LOCUS       DASZMM010000001         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2474524, whole genome shotgun sequence.
ACCESSION   DASZMM010000001 DASZMM010000000
VERSION     DASZMM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474524"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VG754_00005"
     CDS             <1..721
                     /locus_tag="VG754_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWF17613.1"
                     /translation="VVPISVEDGIAKELHVGLGDELDFDVQGVPVNGKVASLRQVDWR
                     RIQPNFFIVFPLGVLEDAPAMHVLVTRVPSAEESARVQSAVVKAFPNISTIDLRLVLQ
                     TIDSILGKISFVVRFMAMFTVLTGLLVLAGALLTGRYQRIQESILLRTLGASRQQILR
                     ILLVEYFSLGVLAALTGILLAVAAAWALCEFVFKVHFDVAIVPLLAALVVVPGLTMFT
                     GMLMSRGVLNEPPLAILRSAA"
     gene            734..1549
                     /locus_tag="VG754_00010"
     CDS             734..1549
                     /locus_tag="VG754_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17614.1"
                     /translation="MNDPRVRLQTMKAESFFSKYAACPISLISLVLVLASAGCGGNHE
                     SKNTLSQEVIAPKPPGFLVGPLSTLLTNAMAFSARLTIDSPASTQKTHELSGQLLGER
                     NHLIFSPTRGDRTFIWDAHERNGFVLSEALQGFAPFASPMQVTNIATMGETAGPTSDR
                     VNGHPGHEVEVAVSMDDGTTTRFSLWRASDLNGFPVRIKTLSADKPFVLDITDIQPAR
                     LSTNLFSPPDGFTKYSTPEIMASELMFRRAKPKKTDSSNFGEPQPIMKQSVPH"
     gene            complement(1556..3025)
                     /locus_tag="VG754_00015"
     CDS             complement(1556..3025)
                     /locus_tag="VG754_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF17615.1"
                     /translation="MAQDMDAIVREHWFEARSANFNIYSCGATQEVHKLAARLEQFHD
                     AFGLLAGAQAVSSPPITVMAFPDQTAMEPFLPLYQGKPANLSGFFKRSSDENLIVLAL
                     TGTNTISMQVIFHEYTHLLFRRNDHVWPLWLQEGMSEIYSTFEVAGRGVRIGEPIQHH
                     LRFLEHSDLMPLKELLAVEHDSPQYNESEHQGVFYAESWLLTHFLMNGDNPALKARFG
                     NFTKLLRQGVPQEKAFTQALGIPLSSVEAELRRYLARQQFQPIAYVVPVDLSAPRQVA
                     TRPIGQTETCFRLGNELMRIGRLDDAEAQFNHAQKLAPQSPLPDEGLGLLAAVREKPD
                     ESIRHLNECLQHGSVSFLANYIYSEERFHQMGDGHGNYTHIANDAAEDIRGKLQRSIV
                     LMPNFAPAHELLGFLELVQGENLVLAEEQLQRAIQLEPENQWHLLSLAQAQLMRKDPA
                     AARQTLEILRQPLVEAKLRERAAVALKEVDEFEHQRSSR"
     gene            complement(3174..>3626)
                     /locus_tag="VG754_00020"
     CDS             complement(3174..>3626)
                     /locus_tag="VG754_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HWF17616.1"
                     /translation="WALVLLGVMIAIVLELAGIPSLAFAVGVYLPISSSAPVFVGGLV
                     RWLVDQRMRRRLREHDLSEAQLSAESDKSPGVLLASGYIAGGAITGIILGIMLTRLSN
                     VDKKLTDWAEAFNPFYAGPNADWLALLPFVALTGFLYLVGREALLAKK"
BASE COUNT          783 a         1034 c          998 g          811 t
ORIGIN      
        1 ggttgtgccc atctcggttg aggatggcat cgcaaaagag ttgcatgtcg gtctggggga
       61 tgagcttgat ttcgacgttc aaggtgtgcc ggtgaatgga aaggtcgcca gccttcgcca
      121 ggtggattgg cggcgcatcc agccgaattt tttcatcgtg tttccgcttg gtgtcttgga
      181 agacgctccc gcgatgcatg tgctcgtcac gcgtgttccg tcggcggaag aatccgcgcg
      241 tgtccaaagc gcggtggtca aggcgttccc aaatatttcc accattgatc tgcgcttggt
      301 gctgcagacc attgattcca tcctcggcaa aatttctttc gtggtccgtt tcatggcgat
      361 gttcacggtg ttgacagggc tgcttgtgct tgcaggcgca ttgttgacag gccgatacca
      421 acgcatccag gaaagcatcc tgttgcggac gctcggcgct tcgcggcaac agattttgcg
      481 gatactgctc gtggaatatt tttcgcttgg tgtactggcg gcattgaccg gtattctttt
      541 ggctgtggct gccgcatggg cgctttgtga atttgtgttc aaggtgcatt tcgatgtggc
      601 aattgtgccg ttgctggcgg ctttggtggt ggtgcccggg ttgaccatgt tcaccggcat
      661 gttgatgagc cgcggcgtgc tgaacgagcc gccgctggcg atattgcgca gtgcggcgtg
      721 aaagttgaac tgaatgaacg atccacgtgt cagattgcag acgatgaaag ctgaatcttt
      781 tttttcaaaa tacgcggcgt gtccaatctc tttgatttcg ctggtgctgg ttctcgcgtc
      841 agccggttgc ggcggaaacc acgagtctaa aaatacgttg tcgcaggaag tgatcgcgcc
      901 caagccgccg ggatttcttg tcgggcctct ttcgacgctg ctcacaaatg ccatggcatt
      961 cagcgcgcgc ctgacgatcg attcgcctgc gagcacccag aagacgcatg aactttccgg
     1021 ccaactcctc ggcgaaagga accatctgat tttctctccg acacgcggcg accgcacgtt
     1081 catctgggat gcgcatgagc ggaacggctt cgttttgagc gaagcgttgc aaggattcgc
     1141 gccatttgct tcgccgatgc aagtcaccaa catagcgacc atgggcgaaa ctgccggacc
     1201 aacttctgac cgcgtcaacg gccatcccgg acatgaggtc gaagtagccg tttcgatgga
     1261 cgacggcacg acgacccgct tcagcttgtg gcgcgcttcg gatttgaacg gctttcccgt
     1321 gcgcatcaaa acgctgagcg ctgacaagcc attcgtgctg gacatcaccg acatccaacc
     1381 cgccaggctc tcaaccaatt tgttctcgcc gcccgacggc ttcaccaaat attccacgcc
     1441 cgaaatcatg gccagcgaat tgatgttccg cagggcgaaa cccaagaaaa cggattcttc
     1501 caattttggc gaaccgcagc caatcatgaa acaatcagtc ccgcactgat gcaaattacc
     1561 gtgacgagcg ctggtgttcg aactcatcaa cttccttgag agccactgcg gcccgctcgc
     1621 ggagttttgc ttccaccagc ggctgacgca agatttccaa tgtttgccgc gcggcggcgg
     1681 ggtcttttcg catcaactgc gcctgcgcca gcgaaagcag atgccattgg ttttccggtt
     1741 ccaattggat cgcccgttgc aactgctcct ccgcgagaac gaggttttcg ccctggacca
     1801 attccaaaaa accgagcagt tcatgcgccg gagcaaaatt cggcatcaac acgattgacc
     1861 tttggagctt gccacgaatg tcttcggccg cgtcgttcgc gatgtgcgtg taatttccgt
     1921 gaccgtcgcc catctgatga aaccgttcct cggagtaaat gtaattggcg agaaaactca
     1981 ccgaaccgtg ttgcagacat tcgttgagat ggcggatgga ttcatccggt ttttcgcgca
     2041 cagcggccag caagccaagc ccttcgtccg gcagcggact ttgcggcgcg agtttttgcg
     2101 catgattgaa ctgcgcttcc gcatcgtcca accgcccgat tcgcatcagt tcgttgccca
     2161 acctgaaaca agtttccgtc tggccgatgg gtcgcgtcgc cacctgccgc ggcgcggaca
     2221 gatccaccgg cacaacgtaa gcgatgggtt gaaattgctg ccgcgccaga tagcggcgca
     2281 gttctgcttc aacggacgac aacgggattc ccaaggcttg cgtgaacgct ttttcctgcg
     2341 gcacaccctg tcgcagcagc ttcgtgaaat ttccaaaacg cgccttcaac gccgggttgt
     2401 cgccattcat caagaaatgc gtcagcagcc atgattccgc gtaaaacacg ccttgatgtt
     2461 cgctttcgtt gtattgcggc gaatcgtgct ccacggcgag caactccttg agcggcatca
     2521 aatccgagtg ttcgaggaac cgaagatggt gctgaatcgg ttcgccgatg cgcacgccgc
     2581 gtcctgcgac ctcgaacgtg gaataaattt cggacatgcc ttcctgcaac cagagcggcc
     2641 agacgtggtc gttgcgccgg aagagcaggt gcgtgtattc gtgaaaaatg acctgcatcg
     2701 aaatggtatt agttccagtc aacgccagca caatcaggtt ttcgtcgctg cttcgcttga
     2761 agaatccgga gaggttcgcc ggcttgcctt gataaagcgg caggaacggt tccatcgccg
     2821 tctggtcggg aaaagccatc acggtgatgg gcggtgaact caccgcctgc gcgccggcca
     2881 gcaacccaaa agcgtcatga aattgttcca gtcgcgcggc gagtttgtgg acctcctgcg
     2941 tcgcgccgca actatagatg ttgaaattcg ccgaacgcgc ctcgaaccaa tgctcgcgca
     3001 cgatggcgtc catgtcctgc gccatggcgg agaccgatat gaagaatgcg acaaaagcaa
     3061 gcaaacacaa taaagaaccg attgttgacg gcgaccgacg gcggggagct gatggaaaaa
     3121 atgtgtgcat tacaatgcag tcgtaatatt cagatgcgtg taaatcggaa atttcatttc
     3181 ttcgccagta atgcctcccg gccgaccaga tacaaaaatc ccgtcaaggc cacaaacggc
     3241 agcaacgcca gccagtccgc attcggcccg gcgtagaatg gattgaacgc ctcagcccag
     3301 tctgtgagtt tcttgtccac gttcgacaaa cgggtcagca tgatgccaag gatgattcca
     3361 gtgatcgctc cgcccgcgat atagccgctc gccagcagca cgccgggact tttatcgctt
     3421 tccgcggaga gttgcgcttc actcaaatca tgttcgcgca ggcgacgacg catcctttgg
     3481 tccaccagcc agcgcaccaa tccgccgaca aaaaccggcg cggacgatga aatcggcagg
     3541 tacactccca ccgcaaatgc gagcgacgga attcccgcaa gttccaatac aatcgcaatc
     3601 atcacgccaa gcaacaccaa tgccca
//
LOCUS       DASZMM010000002         5519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2199078, whole genome shotgun sequence.
ACCESSION   DASZMM010000002 DASZMM010000000
VERSION     DASZMM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199078"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VG754_00025"
     CDS             <1..598
                     /locus_tag="VG754_00025"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWF17617.1"
                     /translation="PLPYPKDALEPYIDAMTMEIHHDKHHNAYVTNVNKAIAGKPDLE
                     KKTVEELISNLDAVPADIRTAVRNNGGGHANHSLFWKWMAPKAGGAPSGKLADDINAT
                     FGSFDAFKEKFEAAGAGRFGSGWAWLVLNGGKLEIVSTANQDNPIMGKAVAGCEGKPI
                     FGCDVWEHAYYLKYQNKRPDYLKAWWNVVNWPEIAKGY"
     gene            complement(669..2213)
                     /locus_tag="VG754_00030"
     CDS             complement(669..2213)
                     /locus_tag="VG754_00030"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWF17618.1"
                     /translation="MERHFSKPFFNLFSVALGGAAVILALSRVPDERSIPNPSPESHP
                     VAINVQGTPASRGATAVSSYAEAVKRAAPSVVNIYSSKTVTDSDNALLNDPLLRHFFG
                     DHLLGSPDTSSKPHKSQSLGSGVICSSDGYILTDFHVVDNADEIKVNLADGRTEYSAK
                     VIGGDPQTDVAIVKINATNLPTITMGNSDNLQVGDVVLALGNPFGVGQTVTMGIASAV
                     GRGGFGVEDIEDFIQTDASINPGNSGGALVDSQGRLIGINAAILSRSGGNQGIGFAVP
                     VNLAREVMEQILQFGRVMRGYLGVTTQALTPDLAKAFNMPQKQTGALIGEVSPNSPAS
                     AAGLHEGDVITGYNGKAVNDSRELRLLIAQSSPHTNANLQILRHGKIQGCTATLSETP
                     HAKTPLPFLTAKASATGKVANSDESSLDAVEVSDLSGDVRQMLEIPSSINGAVVAKVE
                     PGSDSYEAGLRVGDVITDVNGQTVKNADSAIGLCHRTRGGMALLRVWSKDGKHYIAVN
                     NQVATK"
     gene            2262..2405
                     /locus_tag="VG754_00035"
     CDS             2262..2405
                     /locus_tag="VG754_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17619.1"
                     /translation="MKYVEENAKWGIQPTEAGRNACEKDVRKIFVNIIFMRPSIWFPV
                     GHD"
     gene            2398..3855
                     /gene="dnaB"
                     /locus_tag="VG754_00040"
     CDS             2398..3855
                     /gene="dnaB"
                     /locus_tag="VG754_00040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWF17620.1"
                     /translation="MIDSASENPGEPGDLKKSRRKKAGFSDAAPKTDRLPPHSPEAEQ
                     GVLGCVMLSPNDCLGECVEKFKAGAEVFYDLRHQTIYETLIEMYDQRVPVDIITLQER
                     LKNKQRLEEVGGLVYLTSLPDTVPSAANLNYYLEIVLEKFVLRKMIHTCTEVVSRVYE
                     HEGAVDTLLDEVERDILRISETRVEGKTDKIKDLVKKAISTIEDYHQRQGMLTGVATG
                     FTDLDKMTSGLHGGEMIVVAARPSMGKTSLAMNIAEHVAIQEKVPVGVFSLEMTSESL
                     VLRMLCSRSRVNLRNVREGFLAERDFPKLTDAAGKLVSAPLFIDDSSGLSILQLRAKA
                     RRMSQQYGIKLFVIDYLQLLHSTARRAENRQQEIADISNGIKSLAKELNVPVIVLSQL
                     NRELEKDKDRKPRLSDLRESGAIEQDADVVALLYKPSRSDDEDGGGEEQDAVPVNLLI
                     AKQRNGPTGDVHLTFLKSYTRFESAAKISDEDVRQ"
     gene            3883..>5519
                     /gene="bamA"
                     /locus_tag="VG754_00045"
     CDS             3883..>5519
                     /gene="bamA"
                     /locus_tag="VG754_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HWF17621.1"
                     /translation="MRFLIRHGGILALLFFGWMPFASAQSAYDTILSINVTNIGPQVA
                     SDSLIRANIHIKPGDRYIRSSVDQDVLNLYNTGFFSNIRVTDQRTDRGVVLTFMLQGK
                     FRLLSINFEGNTKYSTAKLMKKISSKPGEPFDERKLFNDCQTIEKMYEKAGYQHTTVK
                     YVAQNIDYNTGQESVTFQVQETPKIKITEVDFIGTHAFSQKKLRRVIKTRRHWMWSWL
                     TRKGFFKKDQFLDDEDTLIDYYRSRGYIDFEIKDVKFINPTPKTMKIEIYVNEGNLYK
                     VGSVSFKGNQLFTTDQIIAGLKRQHLESHSKTNIGVHGFQADVGMTFTPGALNHDIHA
                     IEDFYGSKGYINVRQGANLIVKKIPNTEAGTMDLEYDIKEGEKVYIEKILIKGNVKTK
                     DKVIRRELAVSPGDVFDMVRVRLSKERLEGLGFFERVDTKPEPTETDVPNRDDLIVGV
                     DEKSTGNFTFGAGFNTVESLFGYAEVTQANFDLFNPPYFTGGGQKFRLKVQLGTELQD
                     YEVSFVEPWFLNRKLRLQVDLYRTVANYINLDNLYDVS"
BASE COUNT         1334 a         1515 c         1518 g         1152 t
ORIGIN      
        1 ccctcttcct tatcccaaag acgcgctcga accttacatc gacgcgatga caatggagat
       61 acatcacgac aaacatcaca acgcctacgt cacgaacgtc aacaaagcca tcgccggcaa
      121 acccgacctc gaaaagaaaa ctgtcgagga actcatcagc aacctggacg ccgttcccgc
      181 ggacatccgc accgctgttc gcaacaatgg cggcggacat gccaatcatt cccttttctg
      241 gaaatggatg gcgcccaagg ccggtggtgc gccttccggc aaactcgccg atgacatcaa
      301 cgccacgttc ggcagcttcg atgctttcaa agaaaaattt gaagccgcgg gcgcgggccg
      361 tttcggaagc ggttgggcgt ggctggtcct caacggcggc aagctggaaa tcgtttcaac
      421 cgcgaaccag gacaatccca tcatgggcaa ggccgtcgcc ggttgcgaag gcaaacccat
      481 cttcggctgc gacgtctggg aacatgccta ctacctcaag taccaaaaca agcgcccgga
      541 ttatttgaag gcttggtgga acgtggtgaa ctggcccgag atagcgaagg gctactaagc
      601 ggcgaagacg caaatcgcat cgaagtttat ttaggtgggc gagtttgttg atgcagactc
      661 gcccgttttt atttcgtggc gacttgattg ttgacggcaa tataatgttt gccgtccttg
      721 ctccagacgc gcagcaatgc catgccgccg cgcgttcgat ggcacaaccc gatcgcggag
      781 tcggcgttct tcactgtctg gccattgacg tccgtgatga catcaccgac gcgcaacccg
      841 gcttcataag aatccgagcc gggctcgact tttgccacca cagcaccgtt gatggatgac
      901 ggaatctcaa gcatctggcg cacatctccg ctcaaatccg aaacttccac cgcgtcaaga
      961 gacgactcat ccgagtttgc aactttgcca gttgctgatg ccttggcagt caagaatggc
     1021 aacggtgttt ttgcgtgcgg agtttctgat aaagtcgccg tgcaaccttg gattttgcca
     1081 tggcgaagga tttgcaaatt ggcatttgtg tgtggagaac tctgcgcgat cagcaaccgc
     1141 aattcccggc tgtcattcac agccttgcca ttgtagccgg tgatgacatc gccttcgtgc
     1201 aagcccgccg cggaagcggg ggaattcggt gaaacttcac cgatcaacgc gccggtttgt
     1261 ttttgcggca tattgaaggc cttggccaaa tcgggcgtca gcgcctgcgt tgtgactccc
     1321 agataaccgc gcatcacacg gccaaattgc aagatttgct ccatcacctc gcgcgccaga
     1381 ttgacgggca cggcaaaacc aatgccttga ttgccgccgc tgcggctcag gatggcggca
     1441 ttgatgccga tcagccgccc ttgcgaatca acgagcgccc cgccggaatt gcccggattg
     1501 atcgaggcgt ccgtttgaat gaaatcttca atgtcctcca cgccaaaacc gccgcgcccg
     1561 accgcgctgg caatacccat cgtcacagtc tggcccacgc cgaacggatt gcccaacgcc
     1621 agcaccacgt cgccgacttg caaattgtcg ctgttgccca tggtgatggt cggaagattg
     1681 gtcgcgttga ttttcacgat ggcgacatcc gtttgcggat cgcctccgat gaccttggcg
     1741 gaatattcag tccggccatc ggcgaggttc accttgattt cgtccgcgtt atccacgacg
     1801 tggaaatcgg tgaggatata accgtccgaa ctgcaaatca caccggaacc gaggctttgg
     1861 gacttgtgcg gtttggaaga tgtatctggc gagccgagca gatggtcgcc gaaaaaatgg
     1921 cgcaaaagcg ggtcgttcaa aagcgcgttg tcggaatccg tgactgtctt gctcgaataa
     1981 atgttcacca cgcttggggc ggcgcgcttg accgcctccg cgtaactgct caccgccgtc
     2041 gctcctcgcg acgcgggtgt tccctgcacg ttgatcgcga caggatgact ttcggggctt
     2101 ggattgggga ttgagcgttc atccggcact cgggacagcg caaggatgac ggccgcgcct
     2161 ccaagcgcaa ccgagaatag attgaaaaat ggttttgaaa aatggcgttc catatttacc
     2221 tccgtaacca cccaaaccta acactgaaaa tttcttcacg catgaaatac gttgaagaaa
     2281 acgcaaaatg gggcatccag cccacagagg caggtcggaa tgcctgtgaa aaagatgtgc
     2341 gcaagatttt tgtaaatatc attttcatgc gcccatcgat ttggtttcct gttgggcatg
     2401 attgactccg catccgaaaa tccgggcgag cccggcgatt tgaaaaaatc ccggcgcaaa
     2461 aaagccggtt tttccgatgc cgcgcccaag acggaccgtc tcccgccgca ttcgcctgaa
     2521 gcagagcagg gggtgctggg ttgcgtgatg ctttccccca atgactgcct cggagaatgc
     2581 gtggagaaat tcaaggcggg cgcggaagtt ttttacgacc tgcggcatca aacgatttat
     2641 gaaacgctga tcgagatgta cgaccagcgg gtcccggttg acatcatcac gcttcaggaa
     2701 cggttgaaaa acaagcaacg gctcgaagaa gtcggcgggt tggtctatct cacttcattg
     2761 ccggacacag tcccgtcggc ggcaaacctc aattattatc tggagatcgt cctcgaaaaa
     2821 ttcgtgcttc gcaaaatgat ccacacctgc acggaagtgg tgagccgcgt ctatgagcac
     2881 gaaggcgcgg tggacacgct cctggacgaa gtcgagcgcg acatcctgcg catcagcgaa
     2941 acacgcgtcg aagggaagac cgacaaaatc aaagacctgg tcaaaaaagc catcagcaca
     3001 atcgaggatt atcatcaacg gcaagggatg ctcaccggtg tcgccaccgg gttcaccgat
     3061 ttggacaaaa tgaccagcgg tttgcatggc ggagaaatga ttgttgtcgc cgcgcgtccc
     3121 agcatgggca agaccagtct cgcgatgaac atcgccgagc atgtggccat ccaggaaaaa
     3181 gtgccggtgg gcgtgttcag cctggaaatg acctcggaat cgctcgtgtt gcgcatgctt
     3241 tgctcccggt cgcgcgtgaa tctccgcaac gtgcgcgaag gatttctggc cgagcgggat
     3301 tttcccaagc tgaccgatgc ggcgggcaag ctggtgtccg cgccattgtt catcgacgac
     3361 tcctccgggc tgtccatttt gcaattgcgg gccaaggcgc ggcgcatgtc gcagcagtat
     3421 ggcatcaaat tgtttgtcat tgactacctg caattactcc attctactgc gcggcgagca
     3481 gaaaaccggc aacaggagat tgcggacatc tcgaacggca tcaagtcgtt ggcgaaggaa
     3541 ctgaatgttc ccgtcatcgt gttgagccag ttgaaccgcg agttggaaaa ggacaaggac
     3601 cgcaagccgc ggttgtcgga tctgcgtgaa tcgggtgcca tcgaacagga tgccgacgtg
     3661 gtggcgctct tgtacaagcc aagccgcagt gacgatgaag acggcggtgg cgaggagcag
     3721 gatgcagtgc cggtcaacct gttgatcgcc aagcagcgca acgggccgac gggcgacgtg
     3781 cacctgacat tcctcaaatc atatacgcga tttgaaagtg cagcgaagat cagcgacgaa
     3841 gatgtgcgcc aatgacaaaa tgagcccgga gaagactgac agatgagatt tttgatccgc
     3901 catggcggca ttttggcgct gttgtttttt ggttggatgc ccttcgcgtc ggcgcaatcc
     3961 gcgtacgata caattctcag catcaatgtg acgaacatcg gcccgcaagt ggccagtgat
     4021 tcgctcatcc gcgccaacat ccatatcaaa cccggcgacc gctacatccg ctccagcgtg
     4081 gatcaggatg tcctcaatct ctacaacacc ggtttctttt ccaatatccg cgtgacggac
     4141 cagcgcaccg atcgcggcgt ggtcctcacc ttcatgcttc aaggcaagtt ccggcttctc
     4201 tcgatcaact tcgagggaaa cacaaaatat tccaccgcca agttgatgaa aaaaatctcg
     4261 tccaagccgg gtgagccgtt tgacgagcgg aaacttttca acgattgcca gacgatcgag
     4321 aagatgtacg aaaaagccgg ctaccagcac accacggtca aatatgtggc gcagaacatc
     4381 gattacaata ccggacagga aagcgtgacg ttccaggtgc aggaaacacc caaaataaaa
     4441 ataaccgagg tggatttcat cgggacgcac gcattcagcc agaagaaatt gcgccgcgtc
     4501 atcaagaccc gccggcattg gatgtggtcg tggctgacgc gcaagggatt tttcaagaag
     4561 gaccagtttc tcgacgacga ggacacgttg atcgattatt accgcagccg cggttacatt
     4621 gatttcgaga tcaaggacgt gaaattcatc aatcccacgc ccaagacgat gaagattgag
     4681 atctatgtca atgaaggcaa tttatacaag gtcggttctg tctcattcaa agggaaccag
     4741 cttttcacga cggaccagat catcgcggga ctcaagcggc agcatctcga aagccattcc
     4801 aagacaaaca tcggcgtgca tggctttcag gcggatgtcg gcatgacatt cacgccgggc
     4861 gcgctcaacc atgacatcca tgccatcgag gatttctatg gctcgaaggg ttacatcaac
     4921 gtccggcaag gcgcgaacct gattgtaaaa aaaatcccga acaccgaggc cggcacgatg
     4981 gacctcgaat acgacatcaa ggaaggcgag aaggtctata tcgaaaaaat cctcatcaag
     5041 ggaaacgtca agaccaagga caaggtcatc cggcgtgagt tggcggtttc gcccggtgat
     5101 gttttcgaca tggtgcgcgt ccggctcagc aaggaacgcc ttgaaggtct cggctttttt
     5161 gaacgcgttg acacgaaacc cgagcccacg gagaccgatg tgcccaatcg cgacgacctc
     5221 atcgtgggcg tggacgaaaa gtccaccggc aatttcacct tcggcgcggg tttcaacacg
     5281 gtggaaagtc ttttcggcta tgccgaagtg acgcaggcga atttcgacct ttttaatccg
     5341 ccttatttca cgggcggcgg ccagaaattc cgcctgaagg tgcaattggg cacggagttg
     5401 caggattacg aggtttcgtt cgtcgagccg tggttcctga accgcaagct gcgtttacag
     5461 gtggacctgt accgcactgt ggcgaattac atcaacttgg acaacttgta cgacgtgtc
//
LOCUS       DASZMM010000003         5139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2199152, whole genome shotgun sequence.
ACCESSION   DASZMM010000003 DASZMM010000000
VERSION     DASZMM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199152"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VG754_00050"
     CDS             <1..1131
                     /locus_tag="VG754_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWF17622.1"
                     /translation="TKTFADEMYFCLGCLACMTACPAGVNYAELFEHARAEAEQSGVL
                     NSPRRNFIRNFTLRWLFMDQRRLQLAGYALRLYQQLGLQTLVRRSGILKLLPRRLREL
                     EAMTPQIQPEFSAELISPVTQPSGPKKYRVAVLTGCAQDLIFSDVNRDTVEVLARNGC
                     EVVTPADQLCCGSLHAHNGEWELAQQLARKQIDQFPPEQFDAIITNAAGCGSHLKHYA
                     KLLAGDSAYERRAHLWDEKLKDVHEWLAQIGITPPANSAMPQTVTYHEACHLCHGQKI
                     TTQPRQVLRTIPNLKLVELPESTWCCGSAGIYNLIQPEMANELLDRKLKHIQSTGASV
                     VATANPGCLLQILNGARQQQMPLRVVHPITLLAEAYRRPDQV"
     gene            complement(1201..1650)
                     /locus_tag="VG754_00055"
     CDS             complement(1201..1650)
                     /locus_tag="VG754_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF17623.1"
                     /translation="MSDNFFILVAEDDENDAFILQRAFTKAGILDPFHIVSDGQEVMD
                     YLSGSEKYADRNLYPLPNLAILDIKMPKKNGFEALEWTRQQRHLDSLPVMMLSSSGEP
                     TDIEKAYQLGANAYMFKTADIEQMTTALKKACFFWKETVLASGLAVA"
     gene            1862..2482
                     /locus_tag="VG754_00060"
     CDS             1862..2482
                     /locus_tag="VG754_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17624.1"
                     /translation="MSNTAFHVRRAIVDDLDSLKALWNSMHLPAGELERRLTEFQVAE
                     SDEGALLGAVGLEIIERQGRIHSEAFNDFAIADVLRAQLWERMHSVAVNRGLARLWTS
                     ETAPFWKSNGFHPADGGELKRLPPAWNLVPNAWFTMALRDEDALRASLDMDFNRLKQE
                     GALETQRMLNKARKLKVVAIVLTFLVVSAAIAFCVNLLRHLPMFRR"
     gene            2601..4142
                     /locus_tag="VG754_00065"
     CDS             2601..4142
                     /locus_tag="VG754_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17625.1"
                     /translation="MKTFKFVLSLSIFVVLTALLSSASAQAGTIYVPNYSFESPATQT
                     EEPQITSWQETPQPADLDTNTFQWNEMLGVFANLPQGYPGSIANCDGNQLAFMFAYPQ
                     LGIFQDNNSIDGFSSTPTHAFNAKYEAGRSYQLTVGITSSSYEPLNQGSTLQLSLYYR
                     DANSNIVNVAQTTVTYDTNIFTNLDMLVDFHVAITNVQATDPWAGKNIGIEIVSTAAP
                     QLIGGVWDLDNVRLTEAVYVPNFSFESPATQTEEPQITSWQETPQPADLDTNTFQWNE
                     MLGVFANLPQGYPGSIANCDGNQLAFMFAYPQLGIFQDNNSIDGFSSTPTHAFNAKYE
                     VGKSYQLTVGITSSSYEPLNQGSTLQLSLYYRDANSNIVNVAQTTVTYDTNIFTNLDL
                     LVDFQTTVPGVKATDPCAGKNIGIEIVSTAAPQLIGGVWDLDNVRLVDTVATALSQPA
                     VANGQFSFTLLSEPNAAFNILANTNIAGTNWVSLGTITNTTGSVPFTDTQTNLAARFY
                     RARRL"
     gene            4164..4955
                     /locus_tag="VG754_00070"
     CDS             4164..4955
                     /locus_tag="VG754_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF17626.1"
                     /translation="MQIGNQPSYRRAFTLVELLVVIAIIALLAALLLPSLVSAKERAK
                     NVACMSNLRQLGIAIQSYAEDWGGRIPYGPVAPPYTNPSDFYPSTGSPTSLISLQGGA
                     PVGLGLLLSFYISSQPKILFCPSSDQWVDTTSQLSKVGFQQAQSSYYYRHSGNTLLHD
                     TFAATNNPDHLLLDNLGDNRNGLPIRALAIDAQFLCTPDLAAFGVNPSTHHKQQFANI
                     LFADGHALSEKNGDGRFTVNLASQTDLINAFSEILSVLEQADTQK"
     gene            complement(4956..>5139)
                     /locus_tag="VG754_00075"
     CDS             complement(4956..>5139)
                     /locus_tag="VG754_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWF17627.1"
                     /translation="TLGQYLQPTLKHLPVVEFVSPEKFAEYKRIAEAMGFKHVASGPM
                     VRSSYHADEFHPPAHD"
BASE COUNT         1194 a         1493 c         1250 g         1202 t
ORIGIN      
        1 accaagacct ttgccgatga aatgtatttc tgccttggtt gtctcgcttg catgaccgcc
       61 tgtcccgcag gcgtcaatta tgccgagctt tttgaacacg cccgcgctga agctgaacaa
      121 tccggcgtgc tcaattcacc caggcgcaac ttcatccgca atttcaccct tcgctggctt
      181 ttcatggacc agcgccggtt gcaactggcc ggttacgctt tgcggctata ccagcaactc
      241 ggtttgcaaa ccctcgtgcg gcgcagcggc atcctgaaac tgcttccgcg ccggttgcgc
      301 gaactcgaag cgatgacgcc gcaaatccag ccggaatttt ccgccgagct tatcagccct
      361 gtcacccaac cctccgggcc gaaaaaatac cgcgttgctg tccttaccgg ctgcgcccaa
      421 gaccttattt tcagcgacgt caatcgagat acagtggagg tgctcgcacg caacggttgc
      481 gaagtcgtca cccctgccga ccaactttgc tgcggctcgc tccatgccca taacggcgaa
      541 tgggaacttg cccagcaact tgcccgcaaa cagattgacc agtttccgcc ggaacaattc
      601 gatgccatca tcacaaatgc cgccggatgc ggctcgcact tgaaacatta cgcgaaattg
      661 ctagccgggg attcagcgta tgaacgtcgc gctcatttgt gggacgagaa gttgaaggat
      721 gttcacgaat ggcttgctca gattggcatc actcctccgg caaatagcgc catgccacaa
      781 acggtcacgt atcatgaagc ctgtcatctt tgccatggcc aaaaaatcac gacgcaaccg
      841 cgccaggttt tgcggaccat tccgaatctg aagctggtcg aacttcctga aagcacctgg
      901 tgttgcggca gtgcgggcat ttacaatttg atccagcccg aaatggcgaa cgagttgctc
      961 gaccgcaaat tgaaacacat ccaaagcacc ggcgcgagcg tcgtggccac tgccaatcca
     1021 gggtgtctgc tgcaaatact gaatggcgcg cgccagcagc aaatgccgtt gcgcgtggta
     1081 catcctatta cgcttttagc cgaggcatac cgccgtcccg atcaggtatg aagagaagac
     1141 caaccgccag tttttgtcag caagatttgt taaccgaaca atggtgaatc gaattctcgc
     1201 tcaagcaaca gccaatccag atgccaaaac ggtttctttc cagaaaaagc aggccttttt
     1261 cagggctgtc gtcatctgct cgatatcggc ggttttaaac atgtaggcat tggcgccaag
     1321 ttggtatgcc ttttcgatgt cggtcggttc gcctgatgaa ctcaacatca tcaccggcag
     1381 tgaatccaga tgcctttgtt ggcgtgtcca ttccaacgct tcaaacccgt tcttttttgg
     1441 catcttaatg tcgaggatgg ccaggttggg cagaggatac aaattccgat cggcgtattt
     1501 ttccgaacca gagagataat ccatgacttc ctgcccgtcg ctcacaatat gaaacgggtc
     1561 caggatgccg gctttggtga aggcgcgctg caggatgaaa gcatcattct catcatcttc
     1621 agccaccagt ataaaaaaat tatcgctcat cgtatggaca acttggcttg gggctgcgcg
     1681 ccccgaatga atgagtttat atataactcg tgtattggat tctctcaaac gatttttatt
     1741 caaagctcga aaaaagttta tttgcgcctc gcaaattgca acgaagaacc gctcacgaac
     1801 cagttgaagc ccctgaacaa tctctttacc agtttttact tcttattata ttcgtcctcc
     1861 gatgagcaat acggcttttc acgtgcgccg cgccattgtg gacgatctcg acagcctgaa
     1921 ggcgttgtgg aattcaatgc acttgcctgc cggcgaactg gagcgtcgtc tgacggagtt
     1981 ccaagtggcg gaatcggacg aaggcgcatt gcttggtgcc gttggtctgg agatcatcga
     2041 aaggcaaggg cgcatccaca gcgaagcttt caacgatttt gcgattgccg atgttctgcg
     2101 cgcgcaactt tgggagcgga tgcattcggt ggctgtcaac cgcggccttg cccggttgtg
     2161 gacatcggaa acagcgccgt tttggaagag caacggtttc catcccgcag acggcgggga
     2221 attgaaacgt ctgccgcccg catggaattt ggttcccaac gcatggttca cgatggcgct
     2281 tcgcgatgag gacgcgttgc gcgcttcgct ggatatggat ttcaaccggc ttaaacagga
     2341 aggagcactg gaaacccaac gcatgttgaa caaggcgcga aagctgaaag ttgtcgcaat
     2401 tgttcttaca tttctggtcg tcagcgccgc aattgcattt tgcgtcaatc tgctccgtca
     2461 cctgccgatg tttcgacgtt gatttttttg ccgtcgtcaa aagcgcaatg acacttaaat
     2521 tcctctgctc catagaatct gtttgcgcca atccactctt tccatatatt tacgcgaatg
     2581 tctctgcaat tcagaaagca atgaaaacct tcaaattcgt actcagcctt tccatttttg
     2641 tcgtcctaac cgccctgctc tcttcagcca gcgcccaagc aggaacaatt tacgtgccga
     2701 attattcctt tgaatcgccg gccacccaga ctgaggagcc tcaaatcacc tcatggcagg
     2761 agactccaca accggccgat ttggacacga acactttcca gtggaacgag atgcttggcg
     2821 tctttgccaa tctcccgcaa ggctatccgg gcagcatcgc caattgcgac ggcaaccagc
     2881 ttgcgttcat gttcgcttat cctcaactgg gcattttcca ggacaacaat tccattgatg
     2941 gtttcagcag cacgcccacc catgcattca acgcaaaata tgaagccggc agatcgtacc
     3001 aattgacggt gggcatcacc tccagttcct acgaaccact caatcaaggt tcaaccctgc
     3061 aattgagcct ttattatcgg gacgccaaca gcaacatcgt caacgtcgcc cagacaacgg
     3121 tgacctatga caccaatatt ttcaccaact tggatatgtt ggttgatttt cacgtcgcca
     3181 tcacaaacgt gcaggcgacc gatccttggg caggcaaaaa cattgggatt gaaatcgtct
     3241 ccactgccgc gccgcaactg atcggcggcg tttgggactt ggacaacgtg cgtttgactg
     3301 aagctgtcta tgttcccaac ttttcctttg aatcgccggc cacccaaacc gaggagcctc
     3361 aaatcacctc atggcaggag acgccgcaac cggccgattt ggacacgaac actttccagt
     3421 ggaacgagat gcttggcgtc tttgccaatc tcccgcaagg ctacccgggc agcatcgcca
     3481 attgcgacgg caaccagctt gccttcatgt tcgcctatcc tcaactcggc atcttccaag
     3541 acaacaattc gattgatggt ttcagtagca cgcccactca cgcattcaac gcaaaatatg
     3601 aagtcggcaa atcgtatcaa ttgactgtcg gcatcacctc cagttcctac gaaccgctca
     3661 atcaaggttc aaccctgcaa ttgagccttt attatcggga tgccaacagc aacatcgtga
     3721 acgtcgccca gacaaccgtg acgtatgaca ccaatatttt caccaacttg gatctgttgg
     3781 ttgatttcca aaccacggtt cccggcgtga aggcgaccga tccctgcgca ggcaaaaaca
     3841 tcgggattga aatcgtctcc actgccgcgc cgcaactgat cggcggcgtt tgggacctgg
     3901 acaacgtgcg gctggtggac actgttgcga ccgcattgag tcagccggcg gtcgcaaatg
     3961 gccagttcag cttcacgctt ttgagcgagc cgaatgccgc attcaacata cttgcgaaca
     4021 caaatattgc cggaacaaac tgggtgagtc tcggcacgat cacgaacacc actggctcag
     4081 tgccgttcac cgacacccaa acgaacctgg cggcccgatt ttaccgcgcg cgccgtttgt
     4141 gatatagttg gcgcaatcgc gtcatgcaaa ttggcaatca accttcatat cgccgggcgt
     4201 tcacgctcgt tgaattgctg gttgtcatcg ccatcatcgc tctgctggca gcgttgctgt
     4261 tgccctcgct cgtttcggcc aaggaacgcg ccaaaaacgt cgcctgcatg tcgaacctgc
     4321 gccagcttgg catcgccatc caaagttacg cggaggattg gggcggcagg attccttatg
     4381 gtccggtcgc gccgccatac acgaaccctt cggattttta tccgtccacc ggcagcccga
     4441 cgagcttgat ttcattgcag ggcggcgcgc ctgtggggct gggtttgctc ctttcttttt
     4501 acatcagcag ccagccgaaa attttgttct gtccctcaag cgatcaatgg gtagacacaa
     4561 cgtcgcaact ctccaaggtc ggttttcagc aggcccaatc cagctattac taccggcact
     4621 cgggcaacac ccttctgcac gacacgttcg cggcgacaaa caatcccgac catctgctgc
     4681 tcgataatct cggcgacaat cgcaacggcc tgcccatccg cgccctggcg attgacgcgc
     4741 aatttctttg cacgccggac ctcgccgcct ttggcgtcaa tcccagcacg catcacaagc
     4801 agcagttcgc gaacattctt ttcgccgacg gtcacgccct ttcggagaaa aatggcgacg
     4861 ggcgcttcac cgtcaatctc gccagccaga ccgatctcat caatgccttc agcgaaatcc
     4921 tctccgtgct ggaacaggcg gacacgcaga aataattaat catgcgcagg cggatgaaat
     4981 tcatccgcgt gatacgaact gcgaaccatc gggccgctgg caacgtgttt gaatcccatt
     5041 gcctcggcga tgcgcttgta ttcggcgaat ttttcgggtg aaacaaattc cacgaccggc
     5101 aaatgcttca acgtcggttg cagatattga ccgagcgtg
//
LOCUS       DASZMM010000004         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4673128, whole genome shotgun sequence.
ACCESSION   DASZMM010000004 DASZMM010000000
VERSION     DASZMM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4673128"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VG754_00080"
     CDS             complement(<1..1419)
                     /locus_tag="VG754_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF17628.1"
                     /translation="MNRKLSPAWMLTILSGLNLFNYLDRYVLSAVLTPLQKELHLSNG
                     QAGRIMTAFMIGYFVTSPFFGYLGDRLPRKWLIAVGIFVWSLGTLLTGYATTFAVLLS
                     YRVLVGLGEASYATISPSLISDEFPPERRNNALTIFYVAIPVGAALGSILGGVIAARY
                     SWREAFIWAGAPGLLLALILLPFKEPERGGMEGKAAECAKRPTVTDILNLFRVKNYML
                     VVWGYTAYTFALGAFGFWGPTFLTRVHNVAVKDAGTFFGAVLVVAGLLGTLLTGYATT
                     FAVLLSYRVLVGLGEASYATISPSLISDEFPPERRNNALTIFYVAIPVGAALGSILGG
                     VIAARYSWREAFIWAGAPGLLLALILLPFKEPERGGMEGKAAECAKRPTVVDILNLFR
                     IKDYMLVVWGYTAYTFALGAFGFWGPTFLIRVHNVAVKDAGTFFGAVLVVAGLLGTLI
                     GGFAATAWQKRNPAGYAWTLALS"
     gene            complement(1426..2160)
                     /locus_tag="VG754_00085"
     CDS             complement(1426..2160)
                     /locus_tag="VG754_00085"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HWF17629.1"
                     /translation="MFQRFQTNSAYGWMMLAGIAVSIFFWSRLARRDDRLLMIYVTAL
                     VGAFLGAKLVYILAEGWMHFGAPDMWLQLATGKSILGALLGGYAAVELAKRVVGYRGV
                     TGDWFALIAPLGIVLGRVGCLMHGCCLGKECEPAWFTLRDREGIARWPAVPVEIIFNL
                     AAIAGFFFLRRKQRLVGQHFHLYLIAYGAFRFFHEFLRDTPRVAGAISGYQFAALAVM
                     ALGVAGFIRRRNQFASNDAVLNVPAG"
     gene            complement(2196..2444)
                     /locus_tag="VG754_00090"
     CDS             complement(2196..2444)
                     /locus_tag="VG754_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17630.1"
                     /translation="MTIKPARLGGLLLSAWGQDHGTVYENCFVVSGLAFCIVGCIGML
                     EGFKRANLEKIPIGIVIGVCLAFVEVLIMGFVGCCQTI"
     gene            complement(2519..3949)
                     /locus_tag="VG754_00095"
     CDS             complement(2519..3949)
                     /locus_tag="VG754_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF17631.1"
                     /translation="MKFPPLPILQKETLLKKTRSRCPTCHVSCPAEVWRMEETPAKVY
                     LKRTCPVHGEASVCIASDARFYWLAKGSPQNEGPCCAGGNCGPKNSPLNFDFEKGAAI
                     SAADGSVAGTLGRNANGKGDGTFEKLSTCLALIEIVNSCNLACPTCYANSPVGAGSKV
                     DAVPIEQLKERIQGVIDRKGGIEILQLSGGEPTLHPHFFELVDWAQNHPNIDYLLLNT
                     NGVRIARDAEFARQLAIVSARRRIQIYLQYDGTQSAAQVELRGTDLRSLRESAIERCR
                     EIKLPITLAMTVVPENLGHLWEAIEFGLRYPHIRGISFQPMFKSGRRPDATSAPLNTA
                     DIILAAVEQSGGKLRFEDFTPLPCGDPNCATIGYLLKLNGRIQSVSDFIDFTQVQGFL
                     QDKVRYSLEDLMKCGCESEPLGELLKQFELDESNTFRLFIKPFMDAWTWDEDRIDRCC
                     THVIRPDGKLDSFCRYYSGFAESGGQ"
BASE COUNT         1023 a         1219 c          999 g          770 t
ORIGIN      
        1 cgacaatgcc agcgtccacg cgtatcccgc tggattgcgc ttctgccatg ccgtcgccgc
       61 gaaaccgccg atcaacgttc ccaacaagcc cgcaaccacc aacaccgctc caaaaaatgt
      121 tccggcatct tttacagcca cgttatgcac acgtatcagg aatgtcggcc cccaaaatcc
      181 aaatgcgccc aacgcgaacg tgtatgccgt ataaccccaa accaccagca tgtaatcctt
      241 gatgcgaaac agattgagga tgtccacgac ggtcgggcgc ttggcgcatt ccgcggcctt
      301 cccttccatg ccgccgcgtt ccggttcctt gaacgggagc aaaatcagcg cgagcagcaa
      361 tccgggtgcg cccgcccaga tgaatgcctc gcgccaactg taacgcgccg cgatcacccc
      421 gcccaaaatg ctccccaacg ctgcgccgac cgggattgcc acgtaaaaaa tcgtgagcgc
      481 attgtttcgg cgttccggcg gaaattcatc ggaaatcaaa cttgggctga tggtcgcgta
      541 acttgcttca cccaaaccga ctaacacgcg atagctcagc aacaccgcga atgtcgtagc
      601 atatcctgtc aacaacgttc ccaacaagcc cgcaaccacc agcaccgctc caaaaaatgt
      661 tccggcatct tttacagcca cgttatgcac acgtgtcagg aatgtcggcc cccaaaatcc
      721 gaacgcgccc agcgcgaacg tgtacgccgt gtaaccccaa accaccagca tgtagttctt
      781 gacgcgaaaa agattgagga tgtccgtgac cgtcggtcgt ttggcgcatt ccgcggcctt
      841 tccttccatg ccgccgcgtt ccggttcctt gaacgggagc aaaatcagcg cgagcagcaa
      901 gcccggcgcg cctgcccaga tgaaagcctc gcgccaactg taacgcgccg cgatcacccc
      961 gcccaaaatg ctccccaacg ctgcgccgac cgggatcgcc acgtaaaaaa tcgtgagcgc
     1021 attgttccgg cgttccggcg gaaattcatc tgaaatcaag cttgggctga tggtcgcata
     1081 acttgcttcg cccaaaccga ccagcacgcg ataactcagc aacaccgcga acgtcgtggc
     1141 ataccccgtc aacaacgttc ccaggctcca gacaaaaatg ccgaccgcaa tcaaccattt
     1201 gcgcggcaac cggtcgccaa gataaccgaa aaacggcgag gtcacgaaat acccgatcat
     1261 gaatgccgtc atgatgcgcc ccgcctgccc gttgctcaga tgcagttcct tctgcaacgg
     1321 ggtcagcacc gccgacagca cgtaacgatc gagataatta aaaagattca acccggaaag
     1381 gattgtcagc atccaggcgg ggctgagttt tcgattcatg atttgtcaac cggccgggac
     1441 gttcaatacg gcatcattgc ttgcgaattg atttcgacgg cgaataaaac cggccacgcc
     1501 caacgccatc accgccaacg cggcgaattg atagcccgaa attgcccctg ccacacgcgg
     1561 tgtgtcacgc aaaaattcgt gaaaaaaccg gaaagcgcca tacgcgatca ggtacaaatg
     1621 aaaatgttgt ccgaccagcc gttgtttgcg gcgcagaaaa aagaaaccgg cgatggccgc
     1681 aaggttgaaa atgatttcca ccggcaccgc cggccagcgc gcaatccctt cgcggtcccg
     1741 caaggtgaac catgccggtt cgcattcctt gccaaggcaa caaccgtgca tcaaacatcc
     1801 aacgcggccc agaacgattc ccaacggtgc gatcaacgca aaccaatcgc ctgtcacgcc
     1861 gcgataaccg accacgcgct tggcgagttc caccgccgca tagccgccga gcaatgcgcc
     1921 aaggatcgat ttgcccgtgg ccagttgcag ccacatgtca ggcgcgccaa aatgcatcca
     1981 tccctcggca agtatgtaaa caagctttgc gcccaaaaat gcgccaacca gcgccgtgac
     2041 ataaatcatg agcaaccggt cgtcacgccg cgccaggcgc gaccaaaaaa atatgctgac
     2101 ggctatcccg gccagcatca tccagccgta agcggagttg gtttggaatc tctgaaacat
     2161 ctaaagtgca aaaattgaga tgctcataaa aggtgttaaa tggtttggca acagcccacg
     2221 aaacccatga tcaaaacctc cacaaacgcc aggcaaacgc cgatgacaat gccaattgga
     2281 attttttcca aattggcgcg tttaaatcct tccaacatgc cgatacaacc gacgatgcaa
     2341 aaggccaagc ctgatacaac aaaacaattc tcatagactg tcccatggtc ctgtccccaa
     2401 gccgacagca ataatccgcc aagccgggcg ggtttaatcg tcataaaccc gataggtatt
     2461 gggagaaggg cgagaagaaa ccagccgaat cgcgcaaagg agcttgaggg tctttcactc
     2521 attggcctcc actttcggca aacccggagt aataacggca aaaagaatcc agcttgccgt
     2581 cgggtcgtat cacatgcgtg cagcaacgat cgatgcggtc ctcgtcccat gtccacgcgt
     2641 ccatgaacgg cttgatgaac aaccggaacg tgtttgattc gtcgagttcg aattgtttaa
     2701 gcagctcgcc caacggttcc gattcgcaac cgcatttcat caaatcttcc aggctgtaac
     2761 ggaccttgtc ctgtagaaaa ccctgcacct gggtgaaatc aatgaaatcg ctcacggatt
     2821 gaatgcggcc attaagcttc aggagatagc cgatggtcgc gcaattcgga tcaccgcaag
     2881 gcagcggggt gaaatcttcg aaacgtaatt taccacctga ttgctcgact gccgcaagga
     2941 tgatgtccgc ggtgttcaac ggcgccgagg tggcatcagg ccggcgtccg cttttgaaca
     3001 tcggttgaaa actgatgccg cgtatgtgcg ggtagcgcaa accgaactcg atggcttccc
     3061 acaaatggcc gagattctcc ggcacaactg tcatcgccag cgtgatggga agtttgattt
     3121 cgcggcaacg ctcgatggcg ctttcgcgca acgaccgcaa atccgttccg cgcaattcca
     3181 cttgcgccgc ggattgggtc ccatcgtatt gaagataaat ctggatgcgc cggcgcgcgc
     3241 tgacgatcgc aagctgccgc gcaaactccg cgtccctggc gattcgcacg ccattggtgt
     3301 tcaacagcag gtaatctatg ttcggatgat tttgcgccca atccaccagt tcgaaaaaat
     3361 gcggatgaag cgtcggttcg ccgccggaaa gttgcaaaat ttcgatgccc cctttgcggt
     3421 caataacgcc ctggatgcgt tccttgagtt gctcgattgg cacggcatcc accttcgagc
     3481 ctgcgcccac gggcgagttc gcgtaacaag ttggacacgc cagattgcat gaattgacga
     3541 tctcgatcag cgcaagacag gtggacaatt tttcgaacgt gccgtcgccc ttgccattcg
     3601 cattgcggcc caatgtgcct gccacagaac cgtcggcggc ggaaatggcc gcgccctttt
     3661 cgaaatcaaa attgagcggc gaatttttcg gaccgcaatt tccaccggcg caacaaggac
     3721 cctcattttg cgggctgcct ttcgcgagcc aataaaaacg cgcgtctgaa gcgatgcata
     3781 ccgacgcttc accatgaaca ggacaggtgc gtttgagata aaccttggcc ggcgtttctt
     3841 ccatccgcca aacctccgca ggacacgaga catggcacgt cgggcaacgg ctgcgcgtct
     3901 ttttcagcag cgtctctttt tgcaaaattg gcaatggcgg gaatttcatc gaggaacagg
     3961 agaaaaagca acaggaggaa acaaattcgg ggtgcaaccg gcgcaaataa t
//
LOCUS       DASZMM010000005         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3849045, whole genome shotgun sequence.
ACCESSION   DASZMM010000005 DASZMM010000000
VERSION     DASZMM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3849045"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2394)
                     /locus_tag="VG754_00100"
     CDS             complement(<1..2394)
                     /locus_tag="VG754_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWF17632.1"
                     /translation="MNRSLPIWQIHDSIVGVLRQGNRLVLVAPTGSGKTTQVPQMILD
                     AGLAGDKKIVVLQPRRVAARTVAARVAWERKVQLGAEVGYQIRFDDQTSLGTRICFVT
                     EGILLRWLQDDRTLTDVGVILFDEFHERNLLSDVALALVKQLQQTARSDLKLAVMSAT
                     LDAEPVAGYLSSDTTAACPILVSEGQSFPVEVRHLDSHDERPITEQAADTIERIVNSG
                     APGDILVFMPGMGEINATINACRAMNARERLAFIPLHGELQPEEQDFAFAPNALRKIV
                     VATNVAETSVTIDGIRHVVDSGQARIARYDVERGIGTLAIEEISRASAEQRKGRAGRT
                     APGTCYRLWTQSGHLNRPERNTPEIQRSDLAEVVLLLHSLHIRKAAQFDWLDKPDAAA
                     VERAENLLRTLGALDEATDELTDIGRQMMRLPMHPRYSRMLIEASRSGCVRAAALCAA
                     LVSGRDLLQRLRREDKHIAEARELFEASQDSDFFTLMRAYQFAKKNNFNTESCRRYGI
                     HAQSARQVEQTHQQIVQIAGQQKLLQPDEKADDAALLRCIMAGFIDQLCIRRDLGTLD
                     CDLTEGRTGTLMRESVVQNSPLFVAASIREVPSRGSQNLTLLGLASAVKREWIEETFP
                     QQIHTRVEHLFDRTHKRVSAIKLVRFRDLVIHHEHQRDLDPAASGRCLAESFRKEYFE
                     LPLLNHELKQFIARVNLVCAVMPELEFPAFDDTAVTSHLARAFAGMSLVKEAQATHLR
                     DEFFKHLAKEQLAWLDELAPLNIAWPDGRKLKLLYAEEAVKGGEPNPPELQVKLNECF
                     "
     gene            complement(2500..2799)
                     /locus_tag="VG754_00105"
     CDS             complement(2500..2799)
                     /locus_tag="VG754_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM14 family protein"
                     /protein_id="HWF17633.1"
                     /translation="MPTPLVVLWVYIVLLFAGGLMGFLKAGSKMSLLMATAFAVVLIL
                     CATRVITEPRAPDYIMVFLFIFFAVRLTQSKKFMPNGLMSLITLVAIVLRHIHFR"
     gene            complement(2817..2996)
                     /locus_tag="VG754_00110"
     CDS             complement(2817..2996)
                     /locus_tag="VG754_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HWF17634.1"
                     /translation="MEVSEKIQCPFCGQCFVLEVDTSVPSQRFTTDCEICCRPFEVIA
                     ECEPGEILSLDVQGN"
     gene            3119..>3732
                     /locus_tag="VG754_00115"
     CDS             3119..>3732
                     /locus_tag="VG754_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF17635.1"
                     /translation="MKVPLRVLVVEDSEDDTIVLIRELERANFKPSFQQVFSRQALES
                     ALREQAWDLVISDHLLPGFGSFEALRVTKQIQPDIPFIVLSGVIGEEIAVAVMKAGAN
                     DYVMKNNLTRLAPSIERELREAESRRARKRAEEALQRSRQELEDFFDHASVGLRWVGP
                     DGVILRANQAELDMLGYSHEEYVGHNIAEFHADEGTADEVLRRL"
BASE COUNT          824 a          995 c         1111 g          802 t
ORIGIN      
        1 gaaacattca ttgagcttca cctgcaattc gggcggattc ggttcgccgc ctttgaccgc
       61 ttcttcggcg taaagcaatt tcaatttcct gccatcgggc catgcgatat tgagcggcgc
      121 gagttcatcc agccaggcga gttgttcctt cgcaagatgc ttgaaaaact cgtcacgcaa
      181 atgggtcgcc tgcgcttcct tcacgagcga catgccagcg aaagcgcgcg cgagatggct
      241 cgtgacggct gtatcatcga aagccggaaa ttccagctcc ggcatcactg cgcaaaccag
      301 gttcacccgg gcgatgaact gtttcaattc gtgattgagc aatggcagtt caaagtattc
      361 tttccgaaaa gactcggcga ggcagcgacc ggacgcggca ggatcgaggt cgcgctgatg
      421 ttcgtgatgg atgacgaggt cgcggaagcg gacgagcttg atcgcggaaa cgcgtttgtg
      481 ggtgcggtcg aaaaggtgct ccaccctggt gtgaatctgc tgcggaaacg tttcctcaat
      541 ccactcgcgt ttgacggcgc ttgcaaggcc aagcagcgtt agattttgcg aaccgcgcga
      601 tggcacttcg cggatggacg ccgcaacgaa caacggcgaa ttttgcacca cactttcgcg
      661 catcaaagtc cccgtgcggc cctcggtcaa atcgcaatcc aatgtcccca agtcgcggcg
      721 gatgcaaagc tggtcaatga aaccggccat gatgcagcgg agcagggcgg cgtcgtccgc
      781 tttttcgtcc ggctgtaaaa gcttctgctg cccggcgatc tggacgattt gctgatgcgt
      841 ctgttccacc tgacgcgcgg attgagcgtg gatgccgtag cggcggcagg attcggtgtt
      901 gaaattgttt ttcttcgcga attgatatgc gcgcatgagc gtgaaaaaat ctgagtcctg
      961 gctggcttcg aacaattcgc gcgcctcggc gatgtgcttg tcctcgcggc gaaggcgctg
     1021 gagcagatcg cgtccgctga caagtgcggc gcataacgca gccgcacgca cacagccgct
     1081 gcgcgacgcc tcgatgagca tccgggaata acgcggatgc atcggcagac gcatcatttg
     1141 ccggccgatg tcggtgagtt catccgtcgc ttcatccagc gcgccgagcg tgcgcaaaag
     1201 attttcggcg cgttcaacgg cggccgcatc gggtttgtcg agccagtcga attgcgcggc
     1261 cttgcggata tgcaaggagt ggagcagcaa aaccacttcg gcaagatcgc tgcgctggat
     1321 ttcaggcgtg ttgcgctcgg gccgattcaa gtgaccgctc tgggtccaaa gccggtaaca
     1381 agttcctggc gcagtgcgtc cggcgcgtcc tttgcgttgc tcggcggatg ctcggctgat
     1441 ttcttcaatc gccaaagtgc caatcccgcg ctcaacatca taccgggcaa tgcgcgcctg
     1501 gccgctgtcc acaacgtgcc ggatgccgtc aatggtgaca gaagtttcgg cgacgttggt
     1561 ggcgaccaca attttgcgca acgcattcgg cgcgaatgcg aagtcctgtt cttccggttg
     1621 caattcaccg tgcaacggga tgaacgcaag ccgttcgcgg gcattcatcg cgcggcaggc
     1681 attgatggtg gcgttgattt cccccatgcc cggcatgaaa acgaggatgt cgccgggcgc
     1741 gccggaattg acgatgcgtt cgatggtatc ggcggcttgc tcggttatgg gacgttcgtc
     1801 gtggctgtcg agatggcgga cttcgacggg aaaactttgc ccttcggaaa cgagaatcgg
     1861 acaagctgca gtagtgtcag aagacaaata accagcaacc ggctcggcgt cgagggtcgc
     1921 ggacatgacg gcgagcttga ggtcggaacg ggcggtttgc tggagctgtt tcaccaacgc
     1981 gagcgcgacg tcgctcaaca aattgcgttc gtgaaattcg tcaaagagaa ttacgccaac
     2041 atccgtgagt gtgcgatcat cctgcaacca gcgcaacaaa atgccttccg tgacgaagca
     2101 aatccgcgtg ccgagactcg tctggtcgtc aaaacggatt tgataaccga cctccgcgcc
     2161 gagttgaact ttgcgttccc acgcgacgcg cgcggcaacg gttcgcgcgg caacgcgccg
     2221 cggttgcagc actacgattt ttttgtctcc ggccaacccg gcatcgagaa tcatctgggg
     2281 aacctgggtg gttttgcccg aaccggtcgg cgcgacgagc acaagacgat tgccctggcg
     2341 aagaacgccg acaatcgagt catggatttg ccagatggga agggaacggt tcaattcaaa
     2401 ttaaagattc agatttaaaa tggcggcgat gctgtcttgg ggcaacttga aaaatttggg
     2461 actggcaagt gttttgcccg cgttgcaatg gagcttagtt cagcggaaat gaatgtgccg
     2521 aagcacaatc gcaacaagtg tgatgaggga catcaggccg ttgggcatga atttttttga
     2581 ttgggtcagg cgcacggcga aaaagatgaa cagaaagacc atgatgtaat ctggagcgcg
     2641 cggttcggtg atgaccctcg tcgcgcaaag gatcaggacg accgcaaagg ctgtcgccat
     2701 caaaagggac attttgctgc cggccttgag aaaacccatg agcccaccgg cgaacaacag
     2761 cacgatgtaa acccaaagaa cgacgagtgg cgttggcata tcgctgcgaa tgtggactag
     2821 ttgccctgca catccaagct cagaatttcc ccgggctcgc attctgcaat cacctcgaat
     2881 gggcggcagc aaatctcgca atcagtcgtg aagcgttgcg acggcacgct ggtgtccact
     2941 tccaagacaa agcattggcc gcagaacggg cattgaattt tttccgaaac ttccatgtga
     3001 ttccggtttt aaattacctg tattcgtcga aaatacaacg ctcgcaaatt tgacgtttgg
     3061 ggccattgaa actaacatag gtggttcgcg agcgcggtga agctggtcgg ttttttatat
     3121 gaaagttcca ttacgtgtac tggtcgtgga ggattcggaa gatgatacga ttgttctcat
     3181 acgcgaactg gagcgcgcca atttcaaacc ctctttccaa caagtctttt cccgacaggc
     3241 gctggaatcc gccttgcgcg aacaggcttg ggaccttgtc atcagcgacc atttgctgcc
     3301 gggctttggg agttttgaag cgttgcgcgt gacgaaacaa atccagcctg acatcccctt
     3361 catcgtatta tccggcgtca tcggcgagga aatcgccgtc gccgtgatga aagccggcgc
     3421 gaacgattac gtgatgaaaa ataatcttac gcgcctggcg ccgtccatcg agcgcgaatt
     3481 gcgcgaggca gaaagccgcc gcgcccggaa acgcgccgag gaggctttgc aacggagccg
     3541 gcaggaattg gaggattttt ttgatcacgc gtcggtaggt ttgcgctggg tcgggcccga
     3601 tggcgttatc ctgcgcgcca accaggcgga attggacatg cttggttact cgcacgagga
     3661 atatgtcggt cataacatcg ctgaattcca cgccgatgaa ggcacggcag acgaagtttt
     3721 gcgccgtctg ca
//
LOCUS       DASZMM010000006         5391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_276554, whole genome shotgun sequence.
ACCESSION   DASZMM010000006 DASZMM010000000
VERSION     DASZMM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276554"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VG754_00120"
     CDS             <1..771
                     /locus_tag="VG754_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWF17636.1"
                     /translation="DAEGRLIGINTAILSRSGGYQGIGFAVPINMARTVMESLITHGK
                     VVRGFMGVSIQDVNPLLQQEFKLSEDHGALVGDVTPHSPADKAGLKSGDVIVSFNNKP
                     VDDSRHLKLQVAELAPGSTVPVKIIRDGSQKSVNVTLKELPGSEEVADGKSQSDNASD
                     ALNGVTVGDIDSQIRNELKLPENIKGAVVQQVDQNSPAYEQGLRQGDVIEEINRSSVN
                     NAEDAVKLTEHVKDKVILLKLWSHGASHYLVVDESKSE"
     gene            1001..1669
                     /locus_tag="VG754_00125"
     CDS             1001..1669
                     /locus_tag="VG754_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF17637.1"
                     /translation="MRILVVEDDAKIASFVVNGLKQSGFAVDRCADGEEAHVLASTTS
                     YDAAIVDIMLPKMDGLSLVQQLRKEGKRVPVIILSAKASVDDRVKGLQAGGDDYLTKP
                     FAFSELLARVQALIRRSTNASEPTRLAVGDLTLDLLNREVSRGNEKIELQSREFALLE
                     YLMRHAGRVVTKTMIMEHIWDYSFDPQTNVVDVLVCRLRNKIDRDKTLLHTIRGVGYV
                     LKSA"
     gene            1650..3071
                     /locus_tag="VG754_00130"
     CDS             1650..3071
                     /locus_tag="VG754_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF17638.1"
                     /translation="MFSNPLNRIGRAFSFRLNLWYASIFTLSACALYVFLYVMLAVTM
                     DHKDRDVLDAQLKEYAILYQSGGTRALQNVINASKKQQQQQFFVRVINPRLGVVFLNV
                     PDDWIAFDPHAIEIAGMQFHQAYLRIPKNELQDFTLVSTPPLYDGSVLEVGRSANNRE
                     TVLQPFRRGFIAVMTPIVLLGFIGGAVFAHRAMKPVREIVHTARSIIDTGKLDARVPV
                     RHSDDELDELAQLFNRMLDKNQALIVGMRESLDNVAHDLRTPLTRLRGTAEAALHAKP
                     GADNAREALADCVEESDRVLTMLQTLMDVAEAEAGTMKLSKFPTDFCALWNEVIELYQ
                     YVAEEKKITVTTDFDAPCEALVDPTRMRQVFANLLDNAIKYTAPGGKVLVETRRELAN
                     IVIRVRDNGMGIPVEEQGKIWDRLYRGDKSRSQRGLGLGLSLVRAIVQAHQGKVHVSS
                     KPEQGSEFTVIIPSGESNGQMNK"
     gene            3231..3839
                     /locus_tag="VG754_00135"
     CDS             3231..3839
                     /locus_tag="VG754_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWF17639.1"
                     /translation="MSDELQGKKIAILVDQGFEQVEMTEPRKALEEAGAETDLISPEE
                     KKVKSWETSKWGKSFKVDVPLAEANAQEYDALLLPGGVMNPDKLRAKPEAVAFVKSFF
                     DAGKPVAAICHGPWTVIETGAARGRRMTSWPSLKTDLRNAGADWVDEEVVVDNGLVTS
                     RKPDDIPAFNQKIIEEFREGVHPKEGNRTGGQEAELARHEAG"
     gene            3847..4005
                     /locus_tag="VG754_00140"
     CDS             3847..4005
                     /locus_tag="VG754_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17640.1"
                     /translation="MTFVWLTPLVIFVLFTGVVLLQEGVRRKSLADYQRELYEAANRA
                     EPEAYEKK"
     gene            3992..4486
                     /locus_tag="VG754_00145"
     CDS             3992..4486
                     /locus_tag="VG754_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17641.1"
                     /translation="MKKNKHDIGTRLLIGALAGLAGTLVMDALRNTSQKAVPGAEPPM
                     KVHPGKYFVKKGEQVLPGKLRRKIPRKAEEVAAKSLGVGYGMTFGALYSVLRPRGGKL
                     VRDGVALGLLNWAIGFLGWLPKTGIMPPIQQQEPKQIAMPIAQHAVYGTATVAAYDLL
                     VATV"
     gene            complement(4525..>5391)
                     /locus_tag="VG754_00150"
     CDS             complement(4525..>5391)
                     /locus_tag="VG754_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17642.1"
                     /translation="VNFGQPIRAGDFLSGYPERKKECINQLNDAIEHNIQSLILHIPQ
                     LEQVRVVEAVKRLYLERLRVGAHTISEPLLPRAEELTLTQRIGRAVERVYQTEPERTA
                     NFVNKLDLYEKWRARFKLTDESLLDEPQKRKLARQGALWATMAILGAPIAIYGWAHRI
                     APFCVVAWSIKKFANTEKHKAQTSTAAIIAGTVSFSFFYALYAWIFHLFFGWHATFWY
                     VVSLPFTGIIAHYYVRKLRSLGAGFHDTFVLLRAPTAAQRLLRLRKVLVDEIESVAAA
                     GTPTSDPARVVH"
BASE COUNT         1246 a         1421 c         1591 g         1133 t
ORIGIN      
        1 gatgctgaag gccgtttgat cggcatcaac acggcgatct tgagccgcag cggcggttat
       61 caaggcatcg gtttcgcggt gcccatcaac atggcgcgca cggtgatgga aagcctcatc
      121 actcacggca aagtggtgcg cggcttcatg ggtgtgagca tccaggatgt gaacccgttg
      181 ttgcagcagg aattcaaatt gtccgaggac catggcgctc tcgttggcga cgtgaccccg
      241 cacagcccgg cggacaaggc cggtttgaag agcggcgacg tgatcgtcag cttcaataac
      301 aagccggttg atgacagccg tcatttgaaa ttgcaggtgg cggaactggc gcccggctcg
      361 actgtcccgg tgaaaatcat ccgcgacggt agtcaaaaat ccgtgaacgt cacgttgaag
      421 gaattgcccg gctctgaaga agtcgccgat ggaaagagcc aaagcgacaa tgcaagcgac
      481 gccttgaacg gcgtgacggt cggcgacatt gattcgcaga tccggaacga attgaagctc
      541 cccgaaaata tcaagggcgc ggtcgtgcag caggtggacc agaattcacc cgcttacgaa
      601 cagggattgc gccagggcga tgtcatcgag gaaatcaacc gctcgtcggt aaacaatgcc
      661 gaggacgcgg tgaaacttac agagcacgtc aaagataaag tgattctgct caagctttgg
      721 agccatggtg cgagccacta cctcgtagtt gatgaaagca agtccgaata agccaggttg
      781 agttgaatag aatcgaacaa cttggatgaa tgcacccgcc ctcccgcgcc aaccagccgg
      841 gagggcggtt tttgcagacg gaaacggagt attggaggat tggagttatg ggatgccgtg
      901 ccgagaatcc attagtccaa tactccattt ttgttcctca caccccaact ttttgagtat
      961 gacgaaagcc cctgttgatt tatcatcatc agacagcatt atgcgaattc tggtggtgga
     1021 agatgacgcg aagatcgcct cgttcgtggt gaacggcctc aagcaaagcg gttttgcggt
     1081 ggaccgttgc gccgacggcg aggaagcgca tgtgctcgcc tcgaccacct cgtatgacgc
     1141 ggccatcgtg gacatcatgc tgccgaaaat ggacggcttg agcctcgtcc agcagttgcg
     1201 caaggaaggc aagcgcgtcc cggtcatcat cctgagcgcc aaggcgtctg tggatgatcg
     1261 cgtgaaaggt cttcaagccg gcggtgacga ttatctcaca aagccgttcg cgttttcgga
     1321 attgctggcg cgtgtgcagg cgttgatccg ccgttcaacc aatgcttcgg agccgacgcg
     1381 tcttgccgtg ggcgacctga cgcttgattt gctcaatcgc gaagtttcgc gcggcaacga
     1441 gaagattgaa ttgcaatcgc gggaatttgc gttgcttgaa tatttgatgc gtcacgccgg
     1501 tcgggtggtc accaaaacca tgatcatgga acatatatgg gattacagtt ttgatccgca
     1561 gacgaacgtc gttgacgtgc tggtctgccg gttgcgcaac aaaattgacc gcgacaagac
     1621 gctgctccac accatccgcg gcgtaggtta tgttctcaaa tccgcttaac cgcattggcc
     1681 gcgcgttcag tttccggctg aatctgtggt acgcgtccat cttcacgctc agcgcgtgcg
     1741 cgctttatgt gtttctttac gtgatgctcg ccgtaaccat ggatcacaag gaccgcgacg
     1801 tgctcgacgc gcagctcaag gaatatgcca tcctttatca aagcggcgga acgcgcgcgc
     1861 tgcagaatgt gatcaatgcc agcaagaagc agcagcaaca acaattcttc gtgcgcgtga
     1921 tcaacccgcg actcggcgtc gtgttcctaa atgttcctga cgactggatc gctttcgatc
     1981 cgcacgccat tgaaatcgcc gggatgcagt ttcaccaagc ctatctgcgc attccaaaaa
     2041 atgaactgca agatttcacg ctggtttcca cgccgccgtt gtatgacggc tcggttttgg
     2101 aagtgggccg cagtgcgaac aaccgcgaga cagtgttgca accgttccgg cgcgggttca
     2161 tcgcggtgat gacgcccatc gtgctgttgg gattcatcgg cggtgcggtg ttcgcgcatc
     2221 gcgccatgaa gccggtgcgt gagattgttc acacggcgcg ctccatcatt gacaccggaa
     2281 aactcgatgc gcgcgtgcca gtccgccatt ctgatgacga attggatgaa ctcgcccaat
     2341 tgttcaaccg gatgctagac aaaaaccagg cgttgattgt cgggatgcgc gaatcgctcg
     2401 acaatgttgc gcatgatttg cgaacgccct tgacgcgatt gcgcggcacg gctgaggcgg
     2461 cgttacacgc gaagccgggc gcggacaatg cgcgtgaggc gctcgcggat tgcgtcgagg
     2521 aatccgaccg cgtgctgacg atgttgcaga cgttaatgga tgttgccgaa gccgaagcag
     2581 gcactatgaa gttgagcaag tttccgacgg acttttgcgc gctttggaac gaggtgattg
     2641 aactctatca atacgtcgcg gaagaaaaga aaatcaccgt cacgacagat ttcgacgcgc
     2701 cttgcgaggc attggttgac ccgacacgga tgcgtcaggt gttcgccaac ttgctcgata
     2761 acgcgataaa atacaccgcg cccggcggca aggtgctggt ggagacgcgc cgcgaactgg
     2821 cgaacatcgt gatccgcgtg cgcgacaacg gcatgggcat ccccgtcgag gaacagggaa
     2881 agatttggga ccgcctgtat cgcggcgaca aaagccgctc gcaacgcggg cttggtcttg
     2941 gtctaagttt ggtgcgtgcc atagttcaag cgcatcaggg aaaggtccat gtgtcgagca
     3001 aacctgagca agggtcggaa ttcactgtga tcatcccctc gggtgaatca aacgggcaga
     3061 tgaataagtg aacctcgccc tgctccttga tgcaattcaa atataatttc gttcatcacg
     3121 gcagattggc aacggacaaa aataattttt cacgcgcttt ggggcaaagt ccccattcca
     3181 agaggctggt cgcgcgagta ctctgaagtt attggaggag aattgatttt atgagtgatg
     3241 aactacaagg caaaaaaatc gcgattttgg tggaccaagg ttttgaacaa gtggagatga
     3301 ccgaaccgag gaaggccctg gaagaagcgg gcgcggagac ggacctcatc tcgcccgaag
     3361 aaaaaaaggt gaagagctgg gaaacttcca agtggggcaa gagcttcaag gtggatgtcc
     3421 cgctggcgga ggcgaacgcg caggaatatg acgcgctcct gcttcccggc ggcgtgatga
     3481 atcccgacaa gttgcgcgcg aaaccggaag cggtcgcttt cgtcaaaagc ttttttgatg
     3541 cgggcaaacc ggtcgcggca atttgccatg gaccatggac ggtgattgaa accggcgcgg
     3601 cgcggggacg tcgcatgact tcctggcctt cgctcaaaac ggatttgcgc aatgccggcg
     3661 cggattgggt ggatgaggaa gtggtcgtgg acaacgggtt ggtcaccagc cgcaaaccgg
     3721 atgacattcc cgcgttcaac caaaaaatca tcgaggagtt tcgcgaaggc gttcatccaa
     3781 aggaaggcaa tcgcacgggc ggccaggagg cggaactcgc caggcacgaa gccggctgat
     3841 ttcatcatga ctttcgtctg gctcactccg ttggtgattt tcgtgctgtt cacgggcgtt
     3901 gttcttttgc aggagggcgt ccgccgaaaa tctttggcgg attatcagcg cgaattgtac
     3961 gaagctgcga accgcgccga gccggaggct tatgaaaaaa aataaacatg atattggcac
     4021 gcgactgttg atcggcgcgc ttgcgggttt ggcaggaacg ctcgtgatgg atgcacttcg
     4081 caacacgagc caaaaagcgg tgcccggcgc ggaacctccc atgaaagtcc atccgggcaa
     4141 atacttcgtg aaaaagggcg aacaagtttt gccgggaaaa cttcgccgta agattccccg
     4201 gaaggcggag gaagtcgcag ccaaatcatt gggggtcggt tacggcatga ccttcggcgc
     4261 gctctattcg gtgttgcgcc ccagaggcgg caagctggtg cgcgacggcg ttgcattggg
     4321 tttgctcaat tgggccatcg gttttctcgg ctggctcccg aagaccggca tcatgcctcc
     4381 aatccagcaa caggaaccca aacaaatcgc catgcccatc gcgcaacatg cggtttacgg
     4441 caccgccact gtcgcggcct acgacttgtt ggtggccaca gtttgattag gagcgccggt
     4501 ctcagaaccg gcgctcggtt tttttcaatg aacgacgcgc gccggatcgg atgtcggcgt
     4561 tccggcagcc gcaacggatt caatttcgtc aaccaaaacc tttcgcaacc ggagcaaacg
     4621 ttgcgcagca gtcggcgcgc gcaacaaaac gaacgtgtca tggaatcccg cgcccaaact
     4681 tctcaatttg cgcacataat aatgcgcgat gattcctgtg aacggcagcg aaacgacata
     4741 ccagaaggtc gcgtgccagc cgaagaacag gtgaaaaatc cacgcgtata aggcatagaa
     4801 aaaactgaag cttaccgttc ccgcgatgat ggccgcggtc gaggtttgcg ccttgtgttt
     4861 ctccgtgttc gcaaattttt tgatggacca tgcgaccaca caaaatggcg cgatgcgatg
     4921 ggcccagcca taaatcgcaa tcggcgcgcc gaggatggcc atcgtcgccc ataacgcgcc
     4981 ctgccgcgcc agctttcttt tttgcggctc gtccaaaagc gattcgtcgg tgagtttgaa
     5041 acgcgcgcgc cacttctcgt acaaatcgag tttgttgacg aaattggccg tgcgttccgg
     5101 ctctgtttgg taaacgcgtt cgacagcgcg cccgatgcgc tgcgtgagcg tcaattcctc
     5161 cgcgcgtggc agcaacggtt ccgaaattgt gtgtgcgccc acgcgcaacc gttcgagata
     5221 aagtcgtttg acggcttcaa cgacgcgcac ctgctcaagc tgcgggatgt gcagaatcaa
     5281 cgactggatg ttatgctcga tggcgtcgtt caactggttg atgcattctt ttttgcgttc
     5341 cggataaccg gacagaaaat ccccggcacg gattggctgg ccgaaattca c
//
LOCUS       DASZMM010000007         3086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2752588, whole genome shotgun sequence.
ACCESSION   DASZMM010000007 DASZMM010000000
VERSION     DASZMM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2752588"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VG754_00155"
     CDS             <1..598
                     /locus_tag="VG754_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWF17643.1"
                     /translation="LLLPLALAFGMTAFAAPMAFDFKDPKSVNNVIFKTDAPLESING
                     TATGVSGNVTFDPENPAATKGTIIVEAASLHVPNPTMQKHLQSDKWLDVAKFPNITFE
                     ALNVENVKSDGNKSTADITGTMTIHGVSNKVTVPVRMTYLKDKLHDRLPSLQGDLLVL
                     RTTFSVKRSDYGINKGTFEDKVSDEIELTLSLAGQSPR"
     gene            637..1041
                     /locus_tag="VG754_00160"
     CDS             637..1041
                     /locus_tag="VG754_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaG domain"
                     /protein_id="HWF17644.1"
                     /translation="MSDNVKFVEPPGLGVTVDRVVYNAKAETPPDRPHCFVYFITIHN
                     GTDQPVTIKGRKWVVTNERGEVMALEGQGVVGKTPTIQPGDSFSYNSFHLLNTNTAIA
                     EGSYLGLDALGRRVLTRIPKFKMQVPKGSGRS"
     gene            complement(1053..1271)
                     /gene="infA"
                     /locus_tag="VG754_00165"
     CDS             complement(1053..1271)
                     /gene="infA"
                     /locus_tag="VG754_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWF17645.1"
                     /translation="MAKEEPIELTGVVTQVLPGTMFRVALPNRHEVLAHISGKMRKNF
                     IRISVGDRVNVQMSPYDLGKARITFRHS"
     gene            complement(1425..2480)
                     /locus_tag="VG754_00170"
     CDS             complement(1425..2480)
                     /locus_tag="VG754_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF17646.1"
                     /translation="MTLSWELSSGEYVYSTPAIDQNSNVYVTVSGNKRYADVSGGKLV
                     SADSEGAKRWAFPIRCDIKSSPAIGDDGTIYFGARDRKLYAISPEGKLRWSFTTDSWI
                     DASPAIGTNGTIYFGSWDQKFYALNPDGTKHWEFATGGPIDSSAAIADDGTIYFGSHD
                     KKFYTLKPDGSLKWAFSTEGAIISSPALNCDGTIYFTSVDGKLYALNPDGSKKWAVWT
                     GGVHSSSPVLDAEGNIYIGVNDTFESRKPDGTKKWSFGYPMVEGSAVVSADGMVYYGG
                     VNDGAGILYGFSQNGEPKTFTPVGGYSTGSPSIGHDSMLYVGGYSLLAFKGTGSLAKS
                     CWPKFRGGLRQTGRIEK"
     gene            complement(2561..>3086)
                     /locus_tag="VG754_00175"
     CDS             complement(2561..>3086)
                     /locus_tag="VG754_00175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF17647.1"
                     /translation="TVIAPGGLSLRKGTPYLLESFRLVLKKFPTARLVLSRDIFNNVA
                     PVFAQYTDLPIDWDPWLPHPQLAERMRACDILVLPSLEDGFARTVTEGLACGLPVITT
                     PNTGASDLIIPGKNGEIVPIRDPAAIAEAVFKWADIILSRDGKPEISFDANSLSFEAF
                     EKTFIEQLKTRGLA"
BASE COUNT          726 a          826 c          833 g          701 t
ORIGIN      
        1 gttgctgctt ccgctggcgc tcgcctttgg catgaccgct ttcgccgcgc cgatggcttt
       61 cgatttcaaa gatccgaaga gcgtcaataa cgtcatcttc aagacggatg cgccgcttga
      121 atcgatcaac ggcacggcga cgggagtttc cgggaacgtc acgtttgatc cggagaatcc
      181 tgccgcgacg aagggcacaa tcattgttga agccgcatcg ttgcatgttc ccaatccgac
      241 gatgcagaag catctccaga gcgataaatg gctggacgtg gcgaagttcc cgaacatcac
      301 ttttgaagcg ctcaacgttg aaaacgtgaa aagcgatggc aacaaatcga ctgcggacat
      361 caccgggaca atgacgattc atggcgtttc caataaagtc accgtgcccg tgagaatgac
      421 ttacctgaag gacaagctgc acgatcggtt gccgagtttg cagggtgatc tcttggtttt
      481 gcgcaccacg ttttccgtga agcgaagcga ttatggcatc aacaagggaa cattcgagga
      541 caaggtttct gatgagatcg agttgacctt aagtttggcc ggacaatcac cgcgctgaac
      601 attgcaaaat atgaacagtc aaacaaagtt gtgcccgtga gtgataacgt aaagtttgtc
      661 gagccgcccg gtttgggcgt gacagtggac cgcgtggttt ataatgccaa ggcggaaacg
      721 ccaccggacc gcccgcattg ttttgtttat ttcatcacca tccacaatgg caccgaccaa
      781 ccggtcacaa tcaagggccg caaatgggtt gtcacgaacg aacgtggaga agtgatggcg
      841 ttggaagggc agggtgtggt gggtaaaacg ccgaccatcc agccgggcga ttctttcagt
      901 tacaacagct ttcatctgtt gaacaccaac acggcaattg ccgagggaag ttaccttgga
      961 ttggatgcgt tgggtcgccg cgtgctgacg cggataccga agtttaaaat gcaagttccg
     1021 aagggaagcg gacggagtta accaattact gactaactat gcctgaaggt gatgcgagcc
     1081 ttgccgagat catagggaga catctggacg ttgacgcgat cgccgacgct gatgcggatg
     1141 aaattcttgc gcattttgcc cgaaatatgg gccagcactt cgtgcctgtt gggcagcgcg
     1201 acacgaaaca tggttcccgg caacacctgt gtcacaacgc ccgtcagttc gattggttct
     1261 tctttagcca agcgatttta caactcaagg tttcagttgt ttccagtctt gtccacgccc
     1321 aaggcgcgac tcattttatg gcgcgggaca atgcgcttac tccgccccaa aatcaagcaa
     1381 cttttggcgg caaaatttgg ggaaaattct gaccgttccc acgctcattt ttcgatgcgt
     1441 ccggtctgcc tgagtccgcc gcgaaatttc ggccagcaag actttgccaa gctccctgtt
     1501 cccttgaatg ccagcaatga atatccgccg acatagagca tggaatcgtg gccaatcgac
     1561 ggtgagccgg tgctgtagcc gccgaccggt gtgaaggttt tcggttcacc gttttgactg
     1621 aacccgtaca aaattccggc gccgtcgttc acgccaccat aatagaccat tccatccgcc
     1681 gaaaccaccg cgctcccttc aaccatcgga tacccaaacg accacttctt tgtcccgtcc
     1741 ggcttgcggg actcgaaggt gtcattcacc ccgatgtaga tgttgccttc ggcgtccaaa
     1801 actggtgagg acgaatgcac tccgccggtc cagacagccc actttttgct cccgtccgga
     1861 ttgagcgcgt atagttttcc gtcaacggaa gtgaaataaa tcgtcccatc gcaattcagc
     1921 gccggggaag aaatgatggc tccttccgtc gagaacgccc acttcaacga gccatcaggc
     1981 ttcaacgtgt aaaatttttt gtcgtgcgaa ccgaaataaa tcgtgccgtc gtccgcaatc
     2041 gcggcggaag aatcaatcgg cccgcccgtc gcgaattccc agtgtttggt tccgtcaggg
     2101 ttgagcgcat agaatttttg gtcccagctc ccgaaataaa ttgtgccgtt ggtgccgatg
     2161 gccggcgatg catcaatcca tgagtcggtg gtgaacgacc agcgcagctt tccttcaggg
     2221 ctgatggcgt aaagcttccg gtcgcgcgcg ccgaaataga ttgttccatc gtccccgatc
     2281 gccggcgacg atttgatgtc gcaacggatt ggaaaagccc agcgcttcgc gccttcggaa
     2341 tcagcggaaa ccagtttgcc gccggacaca tcggcataac gtttgtttcc cgagactgtc
     2401 acatagacgt tgctgttttg gtcaatggct ggcgtgctgt acacgtattc tcccgatgat
     2461 aattcccacg ataatgtcaa gtgattcgaa ttttcagccc gaagcattgg tacggaagcc
     2521 aatgtcagca tcgcgaaaat caccttgaaa aaaatccgca tcacgcaagc ccccgggttt
     2581 tcaattgttc gataaaagtt ttttcgaatg cctcgaatga aagcgaattc gcgtcaaacg
     2641 agatttccgg tttgccgtcc cgtgacagga tgatgtcggc ccatttgaaa acggcttcgg
     2701 ctattgccgc cggatcgcgg atgggcacaa tctcgccgtt ttttccggga atgatgagat
     2761 cgctcgcgcc ggtgttgggt gtggtgatga caggcaaacc gcaagccaaa ccttccgtga
     2821 ccgtccgcgc gaagccgtcc tccagcgaag gaagcaccag gatgtcgcac gcgcgcattc
     2881 tttcggcgag ttggggatgg ggcaaccatg gatcccagtc gatcggcagg tcagtgtatt
     2941 gggcgaacac cggcgcgaca ttattgaaaa tgtcgcggct caagacgagg cgcgcggtgg
     3001 gaaatttttt gagcaccaga cgaaaacttt ccaggagata gggcgtaccc ttgcgcaaag
     3061 acaatccacc cggcgcaatg acggtc
//
LOCUS       DASZMM010000008         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2477686, whole genome shotgun sequence.
ACCESSION   DASZMM010000008 DASZMM010000000
VERSION     DASZMM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477686"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VG754_00180"
     CDS             complement(<1..1101)
                     /locus_tag="VG754_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HWF17648.1"
                     /translation="MKTIPPTCPVPDTGPKYWRSMDQLAETPEFRQWVEREFPSGASD
                     LSDPVTRRSFVKLMGASFLLAGFGLTGCRKPVEKIYPFSKMPEGYVHGVAKYYATAMP
                     TRGSAVPLLAKSNDGRPTKLEGNAQHPDSNGGTDRYTQASILDLYDPDRATRFAKNGS
                     TATSTDALTALAEISKKAQANGGAGLYFLAGQSSSPSRQRLQKALAEKLPQAGWHEYE
                     PVDFDIHRQAATQAFGKSVSPYFKIDHAKVIVSLDSDFIGAEENAYLNIGRFAKGRKT
                     RTSGDSISRLYTVEGLMTLTGANSDHRLRIASGLVPAVAARLAVEVMKQAGQGDLGAN
                     AQELAGNNAIAANQKWIVECAKDLLANRGECLV"
     gene            complement(1098..1766)
                     /locus_tag="VG754_00185"
     CDS             complement(1098..1766)
                     /locus_tag="VG754_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWF17649.1"
                     /translation="MSDIFPKWTNRLPLLIITAVLLAGTGVTAGVWYYFTNKYTNVGY
                     MPLQPVAFSHAIHADQLGVDCRYCHNAVEKSWYSNIPASSVCMNCHNQVLKDDPKLAL
                     VRESYQTGKPIPWVQIHKLPDYVYFNHSVHVNRGVSCVECHGQINKMDEVAQAKPLTM
                     AFCLECHRDPAAHLRPPDKITDLNWKWSDNPQVNAEKQQANGQKFVHDWKVESLQNCS
                     ACHR"
BASE COUNT          585 a          737 c          725 g          652 t
ORIGIN      
        1 cacaaggcat tcaccgcgat tggcgagcaa atccttcgcg cattccacaa tccatttttg
       61 gtttgcggcg atggcattgt ttccggcgag ttcctgcgca ttcgctccca agtcgccctg
      121 ccctgcttgc ttcatcacct cgacagccag ccgcgccgca accgcaggga ccaacccgct
      181 tgcgatgcgc aaacgatggt ctgagttcgc accggtcaga gtcatcagtc cttcgacggt
      241 gtataaccgg ctgatcgaat cgccgctggt ccgcgtcttg cggcccttgg cgaaccgccc
      301 aatgttcaga tacgcatttt cctccgcgcc gatgaaatcg ctatcgagcg aaacaatcac
      361 cttcgcgtgg tcgatcttga aataaggtga aaccgatttt ccgaacgcct gcgtcgccgc
      421 ctggcgatgg atgtcaaaat ccaccggctc gtattcatgc cacccggctt gcggcaattt
      481 ttcagccaat gctttttgca accgctggcg cgacggcgag ctgctctgcc ccgccaaaaa
      541 ataaagtcct gcgccgccgt tggcctgcgc ttttttggaa atctccgcga gcgcggtcaa
      601 tgcgtcggtg ctcgtcgcgg tcgaaccgtt ttttgcgaat cgcgtcgcgc ggtccgggtc
      661 gtaaaggtca aggatcgaag cctgcgtgta acggtccgtg ccgccgttgc tgtcgggatg
      721 ctgcgcgttg ccttccagtt tcgtgggacg gccatcattg gatttggcga gcagcggaac
      781 ggcgctgccg cgcgtcggca tggcagtcgc ataatatttt gcgacgccgt gcacataacc
      841 ctccggcatt tttgaaaacg gataaatctt ttcaacaggt ttgcggcaac cggtcagccc
      901 aaaaccggcc agcaaaaacg acgcgcccat caatttgacg aatgagcggc gcgtcaccgg
      961 gtcggataaa tcgctggcgc cggaaggaaa ttcgcgctcg acccattgcc ggaattccgg
     1021 cgtctcggcg agttggtcca tgctgcgcca atatttcggg ccggtgtccg gcaccgggca
     1081 ggtgggagga attgttttca tcggtgacag gcggagcaat tctgcaaaga ttcaaccttc
     1141 caatcgtgga cgaatttctg cccgttggcc tgctgcttct cggcattgac ctgcgggtta
     1201 tcgctccatt tccaattgag atcagttatt ttgtccggcg gacggaggtg cgcggcggga
     1261 tcgcggtgac attccagaca gaaggccatc gtcagcggct tggcttgcgc gacttcgtcc
     1321 atcttgttga tctggccgtg gcattccacg caactgacgc cgcggttcac atggacggaa
     1381 tgattgaaat agacgtaatc gggcaatttg tggatttgca cccagggaat cggcttgccg
     1441 gtctggtaac tttcccggac gagcgcaagc ttgggatcat ccttgagcac ctggttgtgg
     1501 cagttcatgc agacggaact ggccgggatg ttcgaatacc aggatttttc caccgcgttg
     1561 tggcagtaac ggcaatcaac acccagttgg tccgcgtgaa tggcgtgcga aaatgccact
     1621 ggctgcagcg gcatataacc cacgttggtg tatttgttcg tgaaataata ccagacaccc
     1681 gccgtcacgc ccgtgcccgc gagcaacacc gcagtgatga tcaataacgg caaccggttt
     1741 gtccatttgg ggaagatgtc agacatctca aattattagt ttgccagccg caaaatccgt
     1801 cctgctgccg gcatcaaaag gtctaatcac gaggagtgtg attcttgcca acggatgcca
     1861 gccgtcaatt gttttttcgc atttttttcc cgacgcctgg acagttccgc gaaccgactg
     1921 gaagccgcga aacaaacttc ccgctgactg tccaacgcga aattaagacc cttacgcccc
     1981 gctccgcaag aaagtttgtg aaatttttca cgaattaggc tgcattgatt tggcgtataa
     2041 gctttgctga tttaaatata ggaggtcaaa aaatgaattc tcaaaacttc ttttggcgca
     2101 agacgtttgg cgtttgcttt tcagccaccg tggaagcaaa tacgattatg ccgacttttt
     2161 gcgcacatat tcgctgatta actgtaacca acaaagtggt catcaagcct tgccataagt
     2221 ggaacccgaa gttctggttc gggaacttgg atgacccgtt gccgccagcc gagtatcgtc
     2281 ccgacgaccc gcatcgagtt cgaaaatggt attggcgcaa cccgacgcat aacttcaact
     2341 tttatgtcat tgggattgct gacaagacgt ttcgccgcgc cgggcgttgt ccgggagaag
     2401 tttgtaatcc gcggggcggc tggaactgga ctgtctgcaa gtataagtgt ctgcggcttc
     2461 cttttgtatc atgccaaacg cgctctttca gtttctatct gggctggcgc gagcggggta
     2521 acttcggggc aaagattgcc ctttataaag caagaaacaa tccaacatga aaacagttaa
     2581 caagttatct tattgactta acgccgcatg tagcctaccc cttacctata tgactaactt
     2641 gacggattta tctgtggaac aattgaagcg ggccgttgcg gtccgggaac aaatcgcgg
//
LOCUS       DASZMM010000009         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4127904, whole genome shotgun sequence.
ACCESSION   DASZMM010000009 DASZMM010000000
VERSION     DASZMM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4127904"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..2759
                     /gene="clpB"
                     /locus_tag="VG754_00190"
     CDS             156..2759
                     /gene="clpB"
                     /locus_tag="VG754_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWF17650.1"
                     /translation="MQPEKLTIKAQEALQSAQRIAQEHSNQEVGGEHLFAALLGQQDG
                     LIQPLLQKLGVPITKLSADVEQAIQQNVKVQGGSETYIGNTLRRVLDAAEAQANKLKD
                     EYVSTEHLLLGLLAEGGPTLRKIFQSYGIKQSEVLKALADLRGNQRVTDSNPEDKFQA
                     LDKYGRDLTALARAGKIDPVIGRDEEIRRVMQVLTRRTKNNPVLIGEPGVGKTAIAEG
                     LARRIISGDVPESLKNRRLVAMDLSAMIAGAKYRGEFEDRLKAFLKEITASEGRIILF
                     IDELHTLIGAGAAEGAADAANMLKPQLARGELRCIGATTLDEYRKHIEKDAALERRFQ
                     PVYVNEPTVEATIAILRGLKERYEVHHGVRIQDAALVAAATLSHRYITDRFLPDKAVD
                     LIDEAAARLRMELDSMPTEVDKLERQIMQLEIEQAALKKEKDEASRDRLKKLERELAN
                     LKEQSSKLKAQWQNEKAAINATSVINSQLEAARQEQEQAQRNGDLNTAAQIQYGKIPD
                     LQRKLADAQKQLHELQKTGRLLNEEVTEEDIAQVVASWTGIPVSRMLEGEREKLVKME
                     ERLQKRIIGQEEAVKAVANAVRRSRSGLQDPNRPIGSFIFLGPTGVGKTELARALAEF
                     LFDDENAMVRIDMSEYMEKHTVARLIGAPPGYVGYEEGGQLSEAVRRRPYCVVLFDEI
                     EKAHHDVFNVLLQVLDDGRLTDGQGRTVDFKNTIIIMTSNIGSPIIQEFYATGAKGGV
                     KAHLEMEKLVRLELKAHFRPEFLNRVDDVIIFHSLSEEQIAKIVDIQLQRLEQRLAQQ
                     NLRLDLDKSAKKLLAAEGYDPQFGARPLKRAIQELLLDPLAVKVLAGEFKPGEKIKAS
                     AHDGELVLEPE"
     gene            2851..3042
                     /locus_tag="VG754_00195"
     CDS             2851..3042
                     /locus_tag="VG754_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17651.1"
                     /translation="MKNLLLTTLLLASSGCVIHEPSYQIIYTPDKPLTNSRVAFSVPL
                     PVIGPQPSTGSGVEQQASR"
     gene            3058..3372
                     /locus_tag="VG754_00200"
     CDS             3058..3372
                     /locus_tag="VG754_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17652.1"
                     /translation="MKPKAKNAEEDFDQGRYMNLEKKLRDKGQRREPAHRDAAEREYE
                     DNEESRLQRELRFHARGKPVAQRKHHGGETRLAGGRPAHGQELSDNETVHPGLAAWRF
                     WR"
     gene            3583..4035
                     /locus_tag="VG754_00205"
     CDS             3583..4035
                     /locus_tag="VG754_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWF17653.1"
                     /translation="MESVEKSIEVEVPIQEVYNQWTRFERFPEFMEGVEQVKKLDERH
                     LHWVAEIDGKKKEWDAEIYEQIPERRIAWRSTAGMRNAGVVELLRKDAQHTRVTLKVD
                     YEPEVSLEHSDGTLETVTQRVEGDLERFKEILQTRGEPSEPWRGEVHE"
     gene            4148..4459
                     /locus_tag="VG754_00210"
     CDS             4148..4459
                     /locus_tag="VG754_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HWF17654.1"
                     /translation="MESKSASDLDESTEQLLQDLKEVVEDGEELLRAGANELSEKQSA
                     ARAKLSAALANARETGRKIQERTVAGARATDRVIRDNPYQTLGIAFGAGVLIAILLNR
                     K"
     gene            4462..4851
                     /locus_tag="VG754_00215"
     CDS             4462..4851
                     /locus_tag="VG754_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWF17655.1"
                     /translation="MAATDVHPPSLMTLLRRAALTGLSALHNRSELFLVELQEEKNRV
                     IEMFIWIAAVCFSGIMFMGVLTATIILIVPEGSRVYAAGGFALFYLVAAILALLNLKA
                     LVKRAALPFSATVEEVKRDRAWLDSLK"
     gene            4830..5207
                     /locus_tag="VG754_00220"
     CDS             4830..5207
                     /locus_tag="VG754_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17656.1"
                     /translation="MVRLLKVKELDDRKRFLLARSEMYRQTLTLEVANIKFATSLLKR
                     RLKAPKTIFMLLGTLALPAAGFFAGRRGLQKKAKAPPPPTGLLPALFAGWKLFSRLVP
                     LVKKLRPARKPSAQPRQNLYHHQ"
     gene            5460..6020
                     /locus_tag="VG754_00225"
     CDS             5460..6020
                     /locus_tag="VG754_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWF17657.1"
                     /translation="MKIKQLMATAAAAILMVTGAKADDWVNTRLCYDTGHDALFNANE
                     ASLDLFGTYQAFERHFLAWPNTSIHHGNYGGGVGGNYFFTRELGIGAEAAFLAGTGAP
                     HFTDHVGGNLFLRLPIDVAHIAPYVFGGGGRAFNPTWNWYWDFGVGLEFRFNPKMGIF
                     ADARYIWKDEGARDQAALRAGLRLVF"
BASE COUNT         1502 a         1578 c         1799 g         1206 t
ORIGIN      
        1 gtacgggagt caaggcttga ccgagaagcc ggttcagccg tcatcggccc accttgcacg
       61 gttttgggag ttgaattatg gataggtgga gatgaacgga tgtgtgcaat tttggcacac
      121 attttgggat ttattgctaa ccaggcaagg gacttatgca accagaaaaa ctaaccatca
      181 aggcccagga agccttgcaa tccgcgcaac ggattgccca ggaacattcc aaccaggaag
      241 ttggcggcga acatcttttc gccgcgttgc tcggccagca agacggattg atccagccgc
      301 ttttgcaaaa attgggtgtg cccatcacca aattgtccgc cgatgtcgag caagccatcc
      361 aacagaacgt gaaggtgcaa ggcggttcgg aaacctacat cggcaacacg ctccggcggg
      421 tgttggacgc cgccgaagcg caggcgaaca agctcaagga cgaatacgtg agcaccgagc
      481 atctgctgct cggtttgctg gccgaaggcg gcccgacgtt gaggaaaatt ttccaaagtt
      541 acggcatcaa acaatccgaa gtgctcaagg cgttggcaga tttgcgtggc aaccagcgcg
      601 tgaccgattc caatccggaa gacaaattcc aagcgctcga taaatatgga cgcgacctga
      661 ccgcgcttgc gcgcgcggga aaaattgacc cggtcattgg ccgcgatgaa gaaatccgcc
      721 gcgtcatgca ggtgttgacg cgccgcacga aaaacaatcc ggtgctcatc ggcgagccgg
      781 gtgtcggcaa gactgcaatc gccgaaggcc tggcgcggcg catcatcagc ggcgatgtgc
      841 cggagtcgct caagaaccgg cgattggtcg cgatggattt gagcgcgatg atcgccggcg
      901 caaaatatcg cggcgaattc gaggaccggt tgaaggcctt tctcaaagaa atcactgcca
      961 gcgaaggacg catcattttg ttcattgatg aattgcacac gttgatcggc gcgggcgctg
     1021 cggaaggggc tgcggacgcg gccaacatgc tcaagccgca actcgcccgc ggcgaattgc
     1081 gctgcatcgg cgcgaccacg ctcgacgaat atcgcaaaca catcgagaag gacgccgcgt
     1141 tggaacgccg cttccaaccg gtctatgtga atgaaccgac cgtcgaggcg accatcgcga
     1201 tcttgcgcgg attgaaggag cgttacgaag tgcaccatgg cgttcgcatc caggacgcgg
     1261 cgctggttgc ggcggcgaca ttatcgcatc gctacatcac cgaccgtttt ctgccggaca
     1321 aggcggtgga tttgattgac gaagccgcag cgcggttgcg gatggaattg gattcaatgc
     1381 cgaccgaagt ggacaaattg gagcggcaga tcatgcaact tgagatcgag caggccgcgt
     1441 tgaaaaaaga aaaggacgaa gcctcgcgcg accggttgaa gaagttggag cgcgaacttg
     1501 cgaacttgaa ggaacaatcg tccaagctca aggcgcagtg gcagaatgaa aaagccgcca
     1561 tcaacgccac cagcgtcatc aattcgcagc tcgaagccgc gcgacaggag caggaacagg
     1621 cgcagcgcaa tggtgacttg aacaccgccg cccaaatcca gtacgggaaa attcccgacc
     1681 tgcaacggaa actcgccgac gcgcaaaagc agcttcatga attgcaaaag accggccgtt
     1741 tgctgaatga agaagtgacc gaggaggaca tcgcgcaggt ggttgcgtca tggaccggca
     1801 ttccggtgag ccggatgctc gaaggcgagc gcgaaaaatt ggtcaagatg gaagagcgct
     1861 tgcagaagcg catcatcggg caggaagaag ctgtcaaggc cgtcgccaat gcggtgcggc
     1921 gttcacgcag cggattgcaa gatccgaacc ggcccatcgg ctcgttcatt tttctcggtc
     1981 cgaccggcgt cggcaaaacc gaactggcgc gcgcgctcgc ggaatttttg tttgatgacg
     2041 aaaacgcgat ggtgcggatc gacatgagcg aatacatgga gaaacacacc gtcgcgcgct
     2101 tgatcggcgc gccgccggga tatgtcggtt acgaggaggg cgggcaactc agcgaggcgg
     2161 tgcggcgccg gccttattgc gtggtgttgt tcgatgagat cgagaaggcg catcatgatg
     2221 ttttcaatgt gctcttgcag gtgcttgatg atgggcgttt gaccgacggg cagggtcgca
     2281 ccgtggattt caagaacacc atcatcatca tgaccagcaa catcggctcg cccatcatcc
     2341 aggaatttta cgccacgggc gcgaagggcg gcgtgaaggc gcatctcgaa atggaaaaac
     2401 tggtgcggct ggaattgaag gcgcatttcc gccctgaatt tttgaatcgc gtggatgacg
     2461 tcatcatctt ccacagcctg agcgaagagc aaatcgcgaa gattgtggac atccagttgc
     2521 aacgattgga acagcgcctc gcgcaacaaa acctgcggct cgacctggac aagtccgcga
     2581 agaaactgct cgccgctgaa ggttacgatc cgcaattcgg cgcaaggccg ttgaagcgcg
     2641 ccatccagga attgctgctt gacccgttgg cggtcaaggt gcttgcgggc gaattcaagc
     2701 ccggcgaaaa aataaaagct tccgctcacg atggcgagtt ggtgctcgaa cccgaataag
     2761 cgtcctcgca atacccctag gccaaaaccc tatggcggcc ttggcccgcc ttgtcgtatt
     2821 ttttcatcat ataagctggg aggacacaga atgaaaaatc tgttgttgac cacattgctg
     2881 ctggcgtcgt ctggctgtgt gattcacgag cccagctacc agatcattta cacgcccgac
     2941 aaacctttga ccaacagccg ggtggcgttc agcgttccgt tgcccgtcat tgggccgcaa
     3001 ccgtcaacgg gcagtggtgt tgaacagcag gcgtcccgtt aagccggcga aagagatatg
     3061 aaaccaaagg ccaaaaacgc ggaggaagat tttgatcaag gaagatacat gaacctggaa
     3121 aagaagcttc gtgataaggg gcaacgccgg gaaccggcgc atcgcgacgc agcagaaagg
     3181 gaatatgaag acaatgagga aagcagattg caaagggaac tgcggtttca tgcccgcggg
     3241 aaaccggtgg cgcagaggaa acaccatggt ggtgaaactc gtcttgccgg cggccggccc
     3301 gcgcacgggc aggagctttc ggataatgaa acggtacatc ccggtcttgc agcatggcgg
     3361 ttttggcgat gaggtttttt tcccggtgaa aattttcaaa gcgcccgccg ccaatggcgc
     3421 ttgaaagtcg cataaatcgt tgaggcaatt tctagtgatg aaaattccag gaatggcgtc
     3481 tgtctatcaa gcgtgcttgg agccgtttta atggatgttt gcaatgctca aacatcggtc
     3541 actcgaagaa caaacaacaa ccaaaataag gagagcaaac ttatggagag tgtcgaaaag
     3601 tccatcgagg tggaagtccc gatccaggag gtttacaacc aatggacgcg gttcgagcgg
     3661 ttccctgaat tcatggaagg agtggagcaa gtcaagaagc tcgatgaacg gcacctgcat
     3721 tgggttgcgg aaattgacgg caagaaaaag gaatgggacg ccgagattta cgaacagatc
     3781 ccggagcggc gcattgcctg gcgcagcacc gccgggatgc ggaatgccgg tgtggtcgag
     3841 cttttgcgca aggacgcgca acacacccgc gtgaccttga aggtggatta tgagcctgaa
     3901 gtcagtttgg aacattccga cggaactttg gaaacagtca cgcaacgcgt ggaaggcgac
     3961 ttggaacggt tcaaggaaat tttgcaaacc cgcggcgaac caagcgaacc gtggcgcggc
     4021 gaagttcatg aatagctgtt tgccaccttg gtttgggtat taaaccccat ggatgttgcg
     4081 tgcctcgaaa cattagcatc catcaggctt tgaacgaatt ttgaaccggg aacgaaaagg
     4141 aaattatatg gagagcaaat cagcaagtga cttggacgaa tcaacagaac aattgcttca
     4201 agatcttaag gaagttgtgg aagacggcga ggaactcctg cgcgctggcg cgaacgaatt
     4261 aagcgaaaag caaagcgccg cgcgcgcgaa actttcggcc gcattggcga atgcccgcga
     4321 aaccggacgc aagatacagg agcgcaccgt tgccggggca agggcgaccg accgcgtgat
     4381 tcgggacaat ccttaccaga cattgggcat cgcgtttgga gccggcgtgc tgatcgcaat
     4441 cctcctcaac cgcaaataac catggctgcc acagatgtcc acccgccgag tctcatgacc
     4501 ctgctgcgca gggcggcgtt gaccggcttg agcgcgctgc acaaccggag cgaacttttt
     4561 cttgtcgaat tgcaggagga aaaaaaccgg gtgattgaaa tgttcatctg gatcgccgcg
     4621 gtctgtttct caggcatcat gttcatgggc gtgctgaccg ccacgatcat tctaatcgtg
     4681 ccggaaggct cacgggttta tgcggcgggc gggttcgcgt tgttctatct cgtggccgcg
     4741 attttggcgt tgctcaacct caaggcgctg gtgaaaaggg ccgcgcttcc attctccgca
     4801 acagtcgagg aagtcaaaag ggaccgcgca tggttagact ccttaaagta aaagaactcg
     4861 atgaccggaa acggtttctg cttgcccgga gcgagatgta ccggcagacg ctcacgctgg
     4921 aagtcgcgaa catcaaattc gcaacttcgc tgctgaagcg caggctcaag gcgccgaaaa
     4981 ccattttcat gttgctggga acacttgcct tgcccgctgc cgggttcttt gccggacggc
     5041 gcggcttgca gaaaaaagcg aaggctcccc cgcccccaac cgggttgttg ccggccctgt
     5101 tcgcaggctg gaaactcttc agccggttgg tgcccttggt caaaaaactt cggcccgcaa
     5161 gaaaaccttc cgcgcagccg cggcagaacc tttaccatca tcaatgacgt cagtaccgca
     5221 tttgaaaaac ttggagggtg ttttgcccgc gtcacatcta ccgcgttccg gtggggcaac
     5281 tccccatggc ctgacttttt tcgcgaccta tagtcgtaca tgaaattgaa aacgggatcg
     5341 ttgctccagg aaacaatgct gcgggttcag aaggtttcgc tggtctgagt gagatgaaca
     5401 aggaagcaat ttttaacggg taactcatgc ataacctcaa actaacggga tcaaataata
     5461 tgaaaataaa acaactgatg gcaacggcag cagccgcaat tctgatggtg acgggcgcaa
     5521 aggcggacga ctgggtgaac acccgcttgt gttatgacac aggccatgat gcgctattca
     5581 atgccaacga ggcgtcgctt gatctgttcg gcacttatca ggcgtttgaa cggcacttcc
     5641 tggcgtggcc aaacacctcc atccatcacg ggaattatgg cggtggtgtt ggcggcaatt
     5701 atttcttcac ccgtgaattg ggcatcggcg cggaggccgc attcctggcg ggaacgggag
     5761 cgccgcattt caccgatcat gttggcggca atctcttctt gcgcctaccg attgatgtcg
     5821 ctcatatcgc gccttatgtt tttggcggcg gcggccgggc attcaatccg acgtggaact
     5881 ggtactggga cttcggtgtg ggcttggaat tccggttcaa tcccaagatg ggcattttcg
     5941 ctgacgcccg ttacatctgg aaagacgaag gcgcaagaga ccaggcagcc cttcgcgcag
     6001 gccttcgcct ggtgttctaa aaatcattct gatagaaatt actcgcggct ggaaacgcaa
     6061 atttccagcc gcgttttttt cggca
//
LOCUS       DASZMM010000010         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3850391, whole genome shotgun sequence.
ACCESSION   DASZMM010000010 DASZMM010000000
VERSION     DASZMM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850391"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1034)
                     /locus_tag="VG754_00230"
     CDS             complement(135..1034)
                     /locus_tag="VG754_00230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF17658.1"
                     /translation="MKIKRRRFLQQSALGVGGVLLGARFAAADSKPRNFDPYEKVPFG
                     KTKLDVSRLCLGTGMNGGMRQSNQTRMGKEKFEELIHGAYDRGVRLFDMADLYGTHPY
                     LIPTLKDIPRDNYKIISKMWWRKGGIPEPERPDANIVVTRFLKELQTDYIDVLLLHCV
                     TSGKWPEELRKQMDILSGLKEKGMVRALGVSCHSLEALDAAVDEPWVQCVHTRINPDG
                     VQMDGPADKVVPILKRLHAAGKAVTGMKICGAGKWRNDPDKRAESIKFVLGLGCVDVL
                     NVGFEKTSEIDDFAGMVGKTARS"
     gene            1194..2078
                     /locus_tag="VG754_00235"
     CDS             1194..2078
                     /locus_tag="VG754_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HWF17659.1"
                     /translation="MATDKASTHAPLANLEEWEDFLKERYPENGLAGPNADKKEEQFR
                     DYENTARPTVREFYRLNHRHQTFDFVQEKRREFLSLKRKQMGMWEALEFLNTLVDDSD
                     PDTDLSQMEHLLQTAEQIRKDGHPRWFILTGFIHDLGKILCLFGEPQWAVVGDTFPVG
                     VSYSDKIVFHKFFEANPDSENPRYQTRLGIYEENCGLDNVFLSWGHDEYLYNVVKDYL
                     PKEALYMVRYHSFYPAHKEGEYGHLMNDEDREMFKWVKTFNPYDLYTKSAKRPNLKEL
                     RPFYDDLLQEFFPPVLKW"
     gene            complement(2106..2618)
                     /locus_tag="VG754_00240"
     CDS             complement(2106..2618)
                     /locus_tag="VG754_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17660.1"
                     /translation="MLLPTYILVLYGMRNLKQPSGEFRSRKGLAVLPLLLWLLRWQFL
                     IGILSLALTWALIVTPHRDFFVIQADDAALTLNYLWPRSNVRLEWGEVSNAAIDRREF
                     GIFRKLRFCLRVNARGTTYTSQWLPDRKEATRALDFIRLHVSNLQKGEIPPRPGSTGK
                     IRDNRTICVQ"
     gene            complement(2756..4030)
                     /locus_tag="VG754_00245"
     CDS             complement(2756..4030)
                     /locus_tag="VG754_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF17661.1"
                     /translation="MKNSRFNRRQFIQHSAAATAGAFLLGARNSFGKISPNEKLNIGV
                     IGTANRAAEDIKGVQRENIVALCDVDSTFLDAALEKFPGAKRYTDFRKMLEQKDIDAV
                     VVATPDHMHAVATMAALQSGRPVYCEKPLTHTISEVRIVAKAARESGLATQMGNQIHA
                     SKHYRRVVELVKSGAIGPVREVHCWVGSVWDTMPKPAGEPVPPTLDYEQWLGPVEQRP
                     YSKEYHPFKWRRWWVFGGGTLSDFCCHLTDLAFWALDLKAPLTIEAEGPPPDAECAPP
                     WLIVRYEYAARGAAPPVKLIWYSAKRPPIELPKWGNGVLFVGDKGMLLTDYERHVLLP
                     EKDFEGFVPPAPFIKDSIGHHAEWIHACKTKAPTTCNFGYGALLTEAGLLGNVAYRCG
                     KKIEWDAVAMRAKNCPEAGQYIQHHYRSGWKI"
     gene            complement(4056..>4362)
                     /locus_tag="VG754_00250"
     CDS             complement(4056..>4362)
                     /locus_tag="VG754_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HWF17662.1"
                     /translation="DGSGGLYNDEKNDRHPLKFADKPVGEWNRFRIVMVGDKVHVFLN
                     GELVVNNNTLENYWERDKPIYPIGQIELQNHFGPLWFKNIYIREIPREQGVTQASVR"
BASE COUNT         1003 a         1255 c         1135 g          969 t
ORIGIN      
        1 cagggtttca gccgaaatgg attttaaaaa taaaacatcg aacatcgaac attgaacgct
       61 gaacatcgta tgcaagatgc cgaaataatt cagcgttcga tgttgaatgt tggacgttca
      121 gcgtttcccg ctcactaact acgcgccgtc ttgccgacca ttccggcaaa atcatcaatc
      181 tccgatgttt tctcaaaacc cacattcaac acatccacgc agccgagacc gagaacgaac
      241 ttgatgcttt cagcgcgctt gtcaggatcg ttgcgccatt tgccagcgcc gcaaattttc
      301 atgccggtca cagctttgcc ggctgcgtgc aggcgcttga ggattgggac cactttgtcc
      361 gcgggaccat ccatctggac gccgtcggga ttgatgcgcg tgtggacgca ttgcacccaa
      421 ggctcatcga cggcggcgtc gagcgcttcc aacgaatggc atgatacccc gagcgcgcgg
      481 accattccct tttccttcaa tcccgaaagg atgtccattt gcttgcgcaa ttcctccggc
      541 cattttcccg aagtgacgca atgcaacaac agcacgtcaa tgtaatcagt ctgcaattcc
      601 ttcaggaagc gcgtcacgac gatgttcgcg tccgggcgtt ccggttcggg gatgccgcct
      661 ttgcgccacc acattttgga aatgattttg taattgtcgc gcgggatgtc cttgagcgtg
      721 ggaatcaggt acggatgcgt gccgtaaagg tccgccatgt cgaacaaacg cacgccgcgg
      781 tcgtatgcgc cgtggatgag ttcctcaaat ttttccttgc ccatgcgcgt ctggttggac
      841 tgccgcatgc cgccgttcat gccggtgccg aggcacaagc gggacacgtc gagcttcgtc
      901 ttgccgaaag gaactttttc gtaaggatca aaattccgcg gcttggaatc tgcggcggca
      961 aagcgcgcgc ccaacagaac gcctcccacg ccaagcgcgg attgctggag gaaccggcga
     1021 cgtttgattt tcataatgga gaatggttgt gtgaataagg atggcgtaca ctttgatttc
     1081 tgcaagtgga aagcggaatg agtttgcgcc cttttagact tgcgatttgc aacgcgcaag
     1141 gcgagagttc aaaagccctg acgacacgga aaaacaccaa cacgcaaccg tttatggcaa
     1201 ccgacaaagc ttccacccac gcgccgctcg ccaatctcga agaatgggaa gatttcctca
     1261 aggaacgcta tcctgaaaat ggcctcgccg gtcccaacgc tgataaaaag gaggagcagt
     1321 tccgcgatta cgaaaacacc gcgcgcccga ccgtccgcga attttaccgg ctcaatcacc
     1381 gccatcaaac gttcgatttc gtccaggaaa agcgccgcga atttctctcg ctcaaacgca
     1441 agcagatggg catgtgggaa gcgctggaat ttttaaacac gctcgtggat gacagcgacc
     1501 cggacacaga cctttcgcag atggaacacc tgctgcaaac cgccgagcaa atccgcaagg
     1561 acggacaccc gcgctggttc atcctcaccg gcttcatcca cgatctcggc aaaattcttt
     1621 gcctgttcgg cgaaccgcaa tgggccgtgg tcggcgatac ttttcctgtc ggcgtcagtt
     1681 attccgacaa gattgttttc cacaaatttt ttgaagcgaa tcccgacagt gagaacccgc
     1741 gctatcaaac gcgccttggc atctatgaag agaattgcgg gctggacaac gtcttccttt
     1801 cctggggcca cgacgaatat ctctataacg tggtgaagga ttatctgccc aaggaagcgc
     1861 tttacatggt ccgctaccac tccttttatc ccgcgcacaa ggaaggcgaa tacggccacc
     1921 tgatgaacga tgaagaccgc gagatgttca agtgggtcaa gacattcaac ccttacgacc
     1981 tctacaccaa aagcgcgaaa cgcccaaacc tcaaggaact ccgcccgttc tacgacgact
     2041 tgctccagga atttttcccg ccggtgttga agtggtagct tgccacgtaa ggcccaatca
     2101 agcagttatt gaacacagat ggtcctgttg tcgcgaatct tgcctgtgct accgggacgt
     2161 ggcggaattt cccccttttg taaattgcta acatgaagcc ggatgaaatc caaggcacgg
     2221 gttgcttctt tacgatccgg gagccattgg ctggtgtaag tggtgccacg tgcgttaacc
     2281 cgcaagcaaa acctcagctt gcgaaagata ccaaattctc gcctatcaat agctgcgttc
     2341 gatacttccc cccactcgag gcgcacgttt gatcttggcc ataggtaatt caaggttaac
     2401 gcggcatcgt cagcctggat tacaaaaaaa tctcggtgtg gggtcacaat caaagcccac
     2461 gtcaacgcca agcttaaaat accaatcaag aactgccagc gaagcagcca aagaagcaaa
     2521 ggcagaacgg caagaccttt gcggctccga aattcaccgg aaggctgttt taagttgcgc
     2581 attccgtaca ggacaaggat gtaggtcgga agcagcatgc ctccaatgag catcggccac
     2641 ggactggaaa gtatgactct catagtttcg tgcaggtttg attcctctcg cactgccctt
     2701 gaggcaacgg aatgctcgcc ctgccgggct cagggacttg cgcccatgca aaagattaaa
     2761 tcttccaccc cgaacggtaa tgatgctgga tgtattgccc ggcttcggga caattcttcg
     2821 cgcgcatggc gacggcgtcc cattcgattt tcttgccgca acgatacgcg acatttccca
     2881 gcagtccggc ttccgtcagc aacgcgccat atccgaaatt gcaggtcgtc ggcgctttcg
     2941 ttttgcaggc gtggatccat tccgcatgat ggccgatgga atctttgatg aacggcgcgg
     3001 gcggcacgaa tccctcaaaa tctttctccg gcaacagcac gtgccgttcg taatcggtca
     3061 gcaacattcc cttgtcgccg acgaacaaca cgccgttgcc ccatttcggc agctcaatgg
     3121 gcggtcgttt cgcggaatac caaatcagct tcaccggcgg cgcggcaccg cgcgcggcat
     3181 attcgtaacg cacgatcaac cacggcggcg cgcactccgc atccggcggc ggaccttcgg
     3241 cttcgatggt caacggcgct ttcaaatcga gcgcccaaaa cgcgaggtcc gtcaggtgac
     3301 aacaaaaatc cgacagcgtg ccgccgccaa agacccacca acgccgccat ttgaacggat
     3361 gatattcctt gctgtaagga cgttgctcca ccggcccaag ccattgctca taatccagcg
     3421 tgggagggac cggttcgccg gcgggtttcg gcatcgtgtc ccaaaccgag cccacccagc
     3481 aatgcacttc gcgcaccggt ccgatcgcgc cgctcttcac caattcaacg acgcggcggt
     3541 aatgtttcga cgcatggatt tgattgccca tctgggttgc aagaccggat tcgcgcgcgg
     3601 cctttgccac gatgcgcact tcagaaatcg tatgcgtcag cggtttctcg caataaaccg
     3661 gacgtccgct ttgcaatgcc gccatcgtgg cgacggcgtg catgtggtcg ggcgtcgcca
     3721 ccaccacggc atcaatgtcc ttttgttcga gcatctttcg gaaatccgtg tagcgcttcg
     3781 caccgggaaa tttttccaat gccgcgtcca aaaacgtcga atccacatca cagagcgcga
     3841 cgatgttttc gcgctggacg cctttgatgt cctccgcggc gcgattggcg gtgccgatga
     3901 cgccgatgtt gagtttttcg tttggcgaaa ttttcccgaa agaatttcgc gcgccgagca
     3961 agaacgcgcc cgcggtcgca gccgccgaat gctggatgaa ttggcggcga ttaaatctgc
     4021 tgtttttcat accatgatta aaaggaaagc atcggtcagc gtacactggc ctgcgtcacg
     4081 ccctgttcgc gcgggatttc gcggatgtag atgttcttga accaaagcgg gccgaaatgg
     4141 ttctgcaatt cgatctggcc gatgggatag atgggcttgt cgcgttccca ataattttcc
     4201 agcgtgttgt tgttcaccac cagttcgccg ttcaggaaaa cgtgcacctt gtcgccgacc
     4261 atgacgatgc gaaaacggtt ccattcaccg actggtttgt cggcgaattt gagcggatgc
     4321 cggtcgttct tttcgtcatt gtaaagaccg cccgaaccgt cc
//
LOCUS       DASZMM010000011         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1927271, whole genome shotgun sequence.
ACCESSION   DASZMM010000011 DASZMM010000000
VERSION     DASZMM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927271"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3369)
                     /locus_tag="VG754_00255"
     CDS             complement(<1..3369)
                     /locus_tag="VG754_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWF17663.1"
                     /translation="MLLFVLAFIPNARAEQVLELFNCTWPQVMQKMPEIAEAGYDALW
                     LPPPAKGASGGYSVGYDLFDPFDLGNLNQAGSVSTHYGTEAQLLQMVQVAHRFGIRVY
                     FDNIMNHRGTTVPGYNAQTPTNFYPALIPQDFHLQTTTNGYYVNWPSVQDYNNQWDVQ
                     NEPLSGLIDLANEPGSINDNFGPTLGSTTNKPFFVRQPFNPAYYMDTNGVSLGGPWHP
                     FDGHGQPVAEDVSAYLIRAAMWTLYTTKCDGFRLDAVKHVPSAFFGDSSPSFNGYVGG
                     IQAMYDYVHGYGSNVVGNGYIEQDDMRNSCFDTEAPRNDAMLFGEHLGPPPSMSEYIL
                     TGMRLLNTPLHNVMNNNLGNPSAGLQGLDSAGYTPPSVNGYACFPVGQGAQFAQNQDA
                     TGAYANHRDLQDAYYFMQQGLGIIYTDNYNQSGPPSYFPSVADANAFGEFGDNQMPEL
                     AWIHNQMARGLTWPRWSDNNIVLFERYDNREGTNTQPQNQDVVLFGMNDDYGYPGDIS
                     FDDGVSRTSDGYYGGMPVSNSRGLGVVVGFPPGSVLVQMAKTSSGANRAYTKLLVHGA
                     TQSLSQAQSTANDSVAQNRLIYVGGQSLAQNGGAVELTIPSGGYVMYGYQWPEASRAA
                     LKSAITLRQGGVDAPRLTIQRQDGANGDSNFNPIYPFQMRGSVDQFGNVIGGSNVASL
                     AYAIDIPVVTNANFDIVVENDASSGNALVKLDGGVDVNSQMGLGPTNGADLRDNKPGY
                     ATDVFLGYEQSAFQFQNGPEKFAARNILSNNIVSLGAETYYYTVGSNNLVVVPGSGYR
                     QTITNQTANWVFHDPTNIVTSQSTNPPSQLFPLAPASGQSAQVWVKVGYQFQINTCYI
                     YYTIDGSNPEGSFGVGQGTTKTVQAFWVNHDNVTNNIDWWKGTIPGQSNGMQVRYKVS
                     LYNHNISQPISDSESSGSKLYGLTQYAITNFSPTNALIWLHNDLNPANTTNGLQEGYH
                     IVRARTFLPRAGKSSVYNTFLQTFYYDAQLPTGVIAYPGADGSNVSSPSYTVVVRTDS
                     STTGVTYNISDSNPNNDDGVTGYPNGNGLSNGAPAFVPAQSVSPSGSLNQQYPNLPQE
                     FHFNYAAVPSNGTATITIHLNKLTTSAYTNRYTAL"
BASE COUNT          878 a         1042 c         1178 g          941 t
ORIGIN      
        1 cagggcggta taacgattgg tataggcgct ggtggtgagc ttgttcaaat ggatggtgat
       61 ggtcgcggtg ccgttgctgg gcacggcggc gtaattgaaa tgaaattcct gcggcaggtt
      121 tggatattgc tggttgagcg aaccgctggg gctgaccgat tgcgcaggaa cgaacgccgg
      181 cgctccgttg ctcaagccgt tgccattcgg ataaccggtc acgccgtcgt cgttgttggg
      241 attgctgtct gaaatgttgt aagtcacgcc tgtcgtgctc gaatcagtcc gaacgaccac
      301 cgtgtaactc gggctggaaa cattggagcc atccgcgccg gggtaagcga tgactccggt
      361 gggcaattgc gcatcgtaat aaaaagtctg gaggaatgta ttgtaaacac tcgatttgcc
      421 cgcgcgcggc aggaaggtgc gcgcccgcac gatgtgatag ccctcttgca agccgttcgt
      481 ggtgttggcc gggttcaagt cgttgtgcaa ccagatgagc gcgttggtcg gactgaaatt
      541 ggtgatcgca tattgcgtga ggccgtagag cttggaaccc gaagattccg aatcggaaat
      601 cggttgtgaa atgttgtgat tataaagcga aaccttgtaa cggacttgca tgccgttgga
      661 ttgtccggga atagtgccct tccaccaatc aatgttgttg gtgacgttgt catggttcac
      721 ccaaaacgcc tggacggttt tcgtcgttcc ctgaccgact ccgaacgagc cttcaggatt
      781 gctgccgtcg atggtgtaat agatgtaaca ggtgttgatc tgaaattgat aacccacctt
      841 gacccagact tgggcggatt ggccgcttgc gggagcgagc ggaaaaagct ggctcggcgg
      901 attggtgctt tggctggtga cgatgttggt gggatcgtga aagacccaat tcgctgtctg
      961 gttggtgatg gtctgccggt aaccggagcc gggaaccacg acaagattat tgctgccaac
     1021 tgtgtaatag taagtctccg cgccgagtga aacgatgttg ttgctgagaa tgtttcgcgc
     1081 ggcaaatttt tccgggccgt tctggaactg gaaagcgctt tgttcgtaac cgaggaacac
     1141 atccgtggca taaccaggct tgttgtcgcg caaatcagcg ccatttgtgg gtcccaagcc
     1201 catctggctg ttgacgtcca cgccgccgtc cagcttgacc aaagcgttcc cggatgaagc
     1261 gtcgttttcc acgacgatgt cgaaattcgc gttggtcacc accggaatgt caatcgcata
     1321 agcaagactc gccacgttcg agccgccgat gacattgccg aactggtcca cactgccgcg
     1381 catctggaac gggtaaatcg gattgaaatt ggaatcgccg ttggcgccgt cctgcctttg
     1441 gatggtcaac cggggagcgt ccactccacc ttggcgcaat gtgatcgcgc ttttcaatgc
     1501 cgcgcggctg gcttccggcc attggtagcc atacatcacg taaccgccgc tggggatggt
     1561 cagctcgacc gcgccgccgt tttgcgcaag gctctgaccg ccgacgtaaa tcagccggtt
     1621 ttgcgcgacg gaatcattgg cggtggactg tgcctggctg agactttgcg tcgcgccatg
     1681 cacaagcaat tttgtgtaag cgcggttcgc gccggaagat gttttggcca tctgcaccaa
     1741 cactgagccc ggtggaaaac caaccaccac gcccagcccg cgcgaattgg agacgggcat
     1801 cccgccgtaa taaccatccg aagtgcgcga aacgccgtcg tcaaaactga tgtcgcccgg
     1861 ataaccataa tcatcattca tgccgaacaa cacgacgtcc tgattttgcg gctgggtgtt
     1921 cgtgccttcg cgattgtcgt agcgttcgaa caggacgatg ttgttgtcgc tccagcgcgg
     1981 ccaggtcaat ccgcgcgcca tttgattgtg tatccaggca agctcgggca tttgattgtc
     2041 gccaaattcg ccgaacgcat tggcgtcagc aaccgagggg aaataactgg gcggacccga
     2101 ttgattgtag ttatcggtgt aaatgatgcc gaggccctgt tgcatgaagt aataggcgtc
     2161 ctgcaaatcg cgatggttgg cgtaggctcc ggtcgcatct tgattttgtg caaactgcgc
     2221 gccttgcccc acaggaaaac aagcataacc attgacgctc ggcggcgtgt aacccgcaga
     2281 gtccaatcct tgaagcccgg cgctgggatt gccgagattg ttgttcatca cattgtgcag
     2341 cggggtgttc aacaatcgca tcccggtgag gatgtattcg ctcatgctcg gcggcggccc
     2401 gagatgttcc ccgaacaaca tggcgtcatt gcgcggcgct tcagtatcga aacaactgtt
     2461 gcgcatgtca tcctgttcga tgtagccgtt gccgaccacg ttgctgccgt agccatgaac
     2521 gtaatcgtac atcgcctgga tgccgccgac gtagccgttg aaactcggcg aggaatcgcc
     2581 aaaaaaagcg gaagggacat gtttgaccgc atcaagacgg aagccatcgc atttggtggt
     2641 gtagagcgtc cacatcgccg cgcggatcaa gtaagcgctc acatcttcgg caacaggttg
     2701 gccatggccg tcgaaaggat gccatgggcc gccgagcgaa acgccgttcg tgtccatgta
     2761 atacgcggga ttgaaaggtt ggcggacgaa gaacggtttg ttggttgtgc tgccaagcgt
     2821 cgggccgaaa ttgtcattga tggagccggg ttcattcgcg aggtcaataa gtccggaaag
     2881 cggctcgttc tggacgtccc attgattgtt gtagtcctgg acggaaggcc agttgacgta
     2941 ataaccattc gtggtggttt ggagatgaaa atcctgcgga atcagcgcag gataaaaatt
     3001 tgtcggcgtt tgcgcattgt agccgggaac ggttgttccg cggtggttca tgatattgtc
     3061 gaaataaacg cggatgccaa aacgatgcgc cacctggacc atctgcaaca gttgcgcttc
     3121 ggtcccgtaa tgcgtcgaga cgctgccggc ctgattgaga ttgcccaaat cgaacgggtc
     3181 aaacaaatcg tagccgaccg agtaaccacc gcttgcgcct ttcgccggcg gcggcagcca
     3241 gagagcgtca taacctgcct cggcgatttc cggcattttt tgcatcactt gcggccaggt
     3301 gcagttgaac aactcaagga cttgttcggc gcgagcattc ggaatgaatg ccaggacgaa
     3361 cagcaacatc aatgcgctgc aatatcgcaa aagtgcatgg aaatatcggt gcattagaag
     3421 tcagtatgag gctacggcga aaataaattc atcaggttac catgcaatgt tgctgccatg
     3481 gtttgcgtgg ctgagatcaa aacataaatt cagcatattg aatgtcggcg cctgagcgac
     3541 acacgtcggg gctattaacc taaaatgctg ccagttcggg taatgacagt ccaccgcata
     3601 tttatgcggt catagcccag gcgcatctca ctattttgcc cgtttctggg cccttgcgga
     3661 ttgtgttgat gggctggcgc acatcgctgt cccctcatcc cggccctctc cactgccgga
     3721 acgattcagt tgaaagggga gcgcacgcgc ctccggcgtg cagtgttcgg cgtcccgccg
     3781 aacacttcgt aaaactgatt tttctgtcca ccaaacagag ctcgaatatc agaagtaaga
     3841 gtcttcgccg ggacagctcg gacagcgcgc gagaagcgtg cgctcccctt tttcatcggc
     3901 atgattccgc gctccaactc acattgactt tggatgaaag ccgggcttta aatcatacag
     3961 cgcatggatc cgatattttt tcataacttg atgctatacc acctgcccgg cgtgttcgga
     4021 tggtttttgg gcaattggg
//
LOCUS       DASZMM010000012         5828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1380886, whole genome shotgun sequence.
ACCESSION   DASZMM010000012 DASZMM010000000
VERSION     DASZMM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380886"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..2818
                     /locus_tag="VG754_00260"
     CDS             248..2818
                     /locus_tag="VG754_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HWF17664.1"
                     /translation="MRMTLYDLLPRDGRAANDVLLGRFLEYTEARGLQLYPAQEAAVL
                     ELFEEKNVILNTPTGSGKSLVAAALHFKAISQGKRSIYTCPIKALVNEKWLALCRDFG
                     ADNVGLSTGDATVNRDAPILCCTAEILANIALREGEHADVCDVIMDEFHYYADRERGV
                     AWQVPLLTLSQARFLLMSATLGDTGFFEEELTRLNGRPTVTVSSGERPVPLEHAYSEL
                     PLAQTLESLAADRKTPVYVVHFTQLEAAQSAQDFTSINISSREDKDAIARAVEGFKFT
                     SPYGPDIKKWLRHGIGLHHAGLLPKYRVLVEQLAQKGLLKIICGTDTLGVGINVPIRT
                     VLFTKLCKFDGQKTGILTARDFHQIGGRAGRKGFDDRGWVVAQAPEHVVENLKLEEKA
                     AKGGKKFVRRQPPDKNFVAWDKNTFTRLIVAPPERLTSRFQVTHAMLLNVLSRRGDGC
                     KAMQQLIARSHESPKARKAHVKRAWQLFRSLVERKIIEFIPETESGSYLRVNVELQND
                     FSMDQTLSLYLLDTIPLLDPQSPDYALDLLTLVESILEDPHIILRKQLDRVKDQKMAE
                     MKMQGIEYDERMEELEKLEHPKPNREFVYSTFNAFADKHPWVGQENIRPKSIAREMFE
                     QFRSFADYIKDYELQRAEGVLLRHLASVHKVLTQTVPDTAKNDALREMEVYLSTMIRQ
                     VDSSLLDEWEKLRDPNYQPRAEAKEVRPPGAEEAAADITRDTRAFTAVIRNRIFTFLR
                     GLVVGDYEQALIAAGWGDSSNEPQDDQFTWTAARLQSAMQSYLAEHEHLCLDPNARNT
                     RHTYVTVSDDKKSWRVQQMLVDPEEANDWVAEFQLDLVASRAKGEPVMRLMKLAPLTA
                     "
     gene            complement(2958..3539)
                     /locus_tag="VG754_00265"
     CDS             complement(2958..3539)
                     /locus_tag="VG754_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17665.1"
                     /translation="MKTNLHNASMFFCCGIMAVLVSGCLGPKVLNETVGHVPATDPKV
                     ELRLKTNSPADVLVIYDDKGAQSERSVRRAFYLYANQKRIGNGRRPQFVNLAQEDHLA
                     TIPIYAEVALKTAAPTNELYAVAETPRRFRLESHGRVVSDFTLPQYDQWITAKKLFLF
                     PAAVGADAVGAAAIAGVAVVYGMCQSGTTVKVH"
     gene            3688..4977
                     /locus_tag="VG754_00270"
     CDS             3688..4977
                     /locus_tag="VG754_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF17666.1"
                     /translation="MNTKQERWLRIIPVALIMYTISYVDRTNITLALEPRISSMMHDL
                     LMDDRLKGQAAGIFFIGYVLLQVPGGWLANHWSARKFISLCLIFWGACAVGCGLTKTF
                     RQFEAMRFMLGVAESGVLPATTVLLANWFPRAELARANGYWNLCQPLAIVASAPVTSI
                     LLQWSGWQKMLMFEGALPFLWLPLWWFLIRDHPREAKWISPDEREFLETTLKKEIAGL
                     QPKGNISLLPTFLKLEIPVMVVIYFLHNCEAYGCMTFFTSGLKARHFTDLQYGILFAI
                     PYAITAIIMVLNSWHSDKTQERRGHVAAVYTLSGVSLIASVLLQNHFWTSYVFMCFAI
                     PGPFAAIAPFWAITAETMPRYAMGLVIGLVNAIGNTGGFAGPYITGWLKKESGGITIP
                     FAALGVGMLVAAGLCFLLPRSRVQNVTAALPVVDASV"
     gene            complement(5138..>5828)
                     /locus_tag="VG754_00275"
     CDS             complement(5138..>5828)
                     /locus_tag="VG754_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17667.1"
                     /translation="IKPYREMKVGKRVIIHADDVIALAQSPDKDVARIKLRCLAAKQN
                     FTRHIPKGATTNSLCADPKLREIDTSDNANSVVTLLSFGPFRFFDGGDLTWNMEERLV
                     CPVNLVGTVDVYQVDHHGTDLSNNPLLVQSLSPTVSVMDNGADKGGNPNSLATMKAIS
                     SIQARYQLHKSFFKGAMENTDDEYIANLERKCAGNCIKLSVASDGKTYTITIPATGHK
                     RTFQTKLDKRD"
BASE COUNT         1294 a         1583 c         1595 g         1356 t
ORIGIN      
        1 caaatcaagc aggaggacat ggagcgggaa aaggcgcgga tccaagacat catgctcaaa
       61 ttatacggca aggacctgct ggacgaaatc atcgaatgaa cctcaacctt tgcaacccct
      121 tgcaatcccg tttcctccca cgtgctcccg ctcaatcccg ccaaaattac ccacccccct
      181 ggcgagccaa gttctgggtt catagaccct taaactttga actttgaact ttgatttctt
      241 acattgcatg cgaatgacgc tttacgatct gctcccgcgt gatgggcgcg cggccaatga
      301 cgtcctgctt gggcgcttcc tcgaatacac cgaggcgcgc ggcttgcagc tttatccggc
      361 gcaggaagcg gccgttctcg aattgttcga ggagaagaac gtcattctca acacgcccac
      421 cggctcggga aaatcgctgg tcgcggcggc gttgcatttc aaggccatct cacaaggcaa
      481 acggtccatt tacacctgtc ccatcaaggc gctcgtgaat gaaaaatggc tcgcgttatg
      541 ccgcgatttc ggcgcggaca acgtcggcct gagcaccggc gatgcgaccg tcaatcgtga
      601 cgcgcccatc ctttgttgca ccgccgaaat tcttgcgaac atcgccttgc gcgaaggcga
      661 acacgcagac gtgtgcgacg tcatcatgga cgaatttcat tattacgccg atcgcgaacg
      721 cggcgtggcg tggcaggtgc cgttgcttac gttgtcgcaa gcgcgttttc tcctcatgtc
      781 cgcgacgctt ggcgacacag ggttttttga ggaggaattg acgcggctta atggccgccc
      841 gaccgtcacg gtttcatccg gagaacgtcc cgtgccgctg gaacatgcct attcagaatt
      901 gccgctcgcc caaacgcttg aaagccttgc cgccgaccgc aagacgccgg tgtatgtcgt
      961 gcatttcacg cagcttgaag ccgcgcagag cgcgcaggat tttacgagca tcaacatcag
     1021 ttcgcgcgag gacaaggacg ccatcgcccg cgccgttgaa ggtttcaaat tcaccagccc
     1081 ttatgggcct gacattaaaa aatggctgcg gcacggcatc ggtttgcatc acgccggctt
     1141 gctgccgaaa tatcgcgtgc tcgtcgagca gctcgcgcaa aagggtttgc tgaaaattat
     1201 ttgcggcacc gacacgctcg gcgtcggcat caacgtgccc atccgcacgg tgttgttcac
     1261 caagctttgc aagttcgacg gccaaaagac cggcattctc accgcgcgcg attttcacca
     1321 gatcggcgga cgcgccggac gcaaaggatt tgatgaccgc ggctgggtcg tcgcgcaagc
     1381 gcctgagcac gttgtcgaaa atctcaagct ggaagaaaag gcggcgaagg gcggcaaaaa
     1441 atttgttcgc cgccagccgc cggacaagaa ttttgtcgcg tgggacaaaa acactttcac
     1501 acgattgatt gtcgcgccgc cggaacggtt gacgtcgcgt ttccaggtca cacatgccat
     1561 gctcttgaat gtgctcagcc ggcgcgggga cggttgcaag gcgatgcagc aattgatcgc
     1621 gcgcagccat gaatcgccca aggcgaggaa agcgcatgtg aaacgggcgt ggcaattgtt
     1681 ccgttccttg gtcgagcgca aaatcatcga gttcattccc gagacggaaa gcggaagtta
     1741 ccttcgcgtc aatgtcgagt tgcaaaacga tttctcgatg gaccagacgt tgtcgcttta
     1801 tttgctcgat acgattccgc tgctcgaccc gcaatcgccg gattacgcgc tcgatttgct
     1861 cacgctggtg gaatccattc tcgaagatcc gcatatcatc cttcgcaagc aacttgaccg
     1921 tgtcaaagac cagaagatgg ccgagatgaa gatgcagggc atcgaatacg acgagcgcat
     1981 ggaggaattg gagaagctcg aacaccccaa gccaaatcgc gaatttgttt attcgacgtt
     2041 caacgccttt gccgacaaac acccgtgggt cgggcaggaa aacatccgcc ccaaatccat
     2101 cgcgcgcgag atgttcgagc agtttcgttc tttcgccgat tacatcaagg actatgagtt
     2161 gcaacgcgcc gaaggcgttc tgctgcgcca tctcgcttcc gttcataaag ttctcaccca
     2221 aaccgttccc gacaccgcga agaacgacgc actccgcgaa atggaagttt atctcagcac
     2281 gatgattcgg caggtggact cgagtttgct tgatgaatgg gaaaaattgc gcgacccgaa
     2341 ttatcagccg cgcgccgaag caaaagaagt ccgtccgccc ggcgcggaag aagctgccgc
     2401 cgacatcacc cgcgacacca gagctttcac cgctgtcatc cgcaatcgca tcttcacgtt
     2461 cttgcgtggt cttgtcgtcg gcgattatga acaggctttg attgctgctg gttggggcga
     2521 ttcatccaat gaacctcaag acgatcaatt cacatggacc gcggcacggc tgcaatccgc
     2581 gatgcaatcc tatctcgccg aacatgaaca cctctgcctt gatcccaacg cacgcaacac
     2641 gcgccacacc tacgtcacgg tttccgacga caaaaaatcc tggcgcgtcc agcagatgct
     2701 cgtcgatccc gaagaagcca acgattgggt cgcggaattc caactggatt tagtcgcttc
     2761 tcgcgcgaaa ggcgagccgg tgatgcgatt aatgaagctg gctccgctga cggcttaatt
     2821 tctctatgaa acagaaggat gcaacgattg gtcttccgcc gctggcagtg agcacattgt
     2881 ccagatcaat attagtgtgg agcagaaggc ggaagcgggc cgtctctgaa cggcaagcta
     2941 agagcaactt cgaatattca atgcacctta acggtcgtgc ccgactggca catgccataa
     3001 accactgcga cacctgcaat cgctgccgct cctaccgcat cagcgccgac agcagcggga
     3061 aaaagaaaca acttttttgc cgttatccat tggtcatatt gcggcagcgt aaaatccgaa
     3121 acgacccggc cgtgcgattc caatctgaat cggcgtggtg tttccgcgac ggcgtacaac
     3181 tcgtttgtgg gagccgcggt tttcaatgca acctcagcat aaatgggtat ggttgcgaga
     3241 tgatcctctt gcgccagatt gacgaattgt ggacgcctgc cattgccaat ccgtttctga
     3301 tttgcataca gataaaacgc gcgccggacg gacctttcgc tctgcgcccc tttgtcatcg
     3361 tagatgacca ggacatcagc gggggaattg gtcttgaggc gcagttcaac tttgggatcc
     3421 gttgcgggaa cgtggcccac ggtctcgttc aacaccttcg gcccaaggca accggagaca
     3481 agcacggcca tgatgccgca acagaagaac attgacgcgt tgtgcaaatt ggttttcatc
     3541 acctcaaggc aggagccgac gtgaggaggc tctaaccctt cgtaattgtt agcctttgca
     3601 aagttaaaaa tcaatctcta aatgcgcggc agaaatagaa taacatggac tctgcgtggt
     3661 gggcttggta cagaaatgcg cactgccttg aatacgaaac aagaacgctg gctgcgcatc
     3721 attccggtcg cgttgatcat gtacaccatt tcgtacgtgg accgcacgaa catcacgctc
     3781 gcgcttgagc cgcgcatttc gtccatgatg cacgacttgc tcatggacga ccgtctcaaa
     3841 ggccaggcgg cgggcatttt tttcatcggt tacgtgctgt tgcaggtccc cggtggatgg
     3901 ctggcaaatc attggagtgc tcgaaaattc atcagcctct gtctgatttt ttggggagcg
     3961 tgcgcggtcg ggtgcggctt gacgaaaaca ttccggcaat ttgaagcgat gcggttcatg
     4021 cttggcgtgg cggaaagcgg cgtgctgccg gcaacgactg tcctgctggc caactggttt
     4081 ccgcgcgcgg aattggctcg cgccaacggt tattggaacc tgtgtcagcc gcttgcgatc
     4141 gtcgcttccg ctcccgtcac cagcatcctg ttgcaatggt ctggctggca aaaaatgctc
     4201 atgtttgaag gcgcattgcc gttcctgtgg ctgccgctct ggtggttctt gatccgggac
     4261 catccgcgcg aagcgaaatg gatttcaccg gacgaacggg aatttttgga aacaacgctg
     4321 aaaaaggaaa tcgccgggct tcagccgaag ggaaatattt cgttgctgcc gactttttta
     4381 aaattggaaa ttcccgtgat ggtcgtgatt tatttcctgc acaattgcga ggcttacggc
     4441 tgcatgactt ttttcacgag cggattgaag gcgcggcatt tcaccgacct gcaatacggc
     4501 attttgttcg ccatccctta cgccatcacg gcgatcatca tggtgcttaa ctcgtggcat
     4561 tcggacaaga cgcaggaacg gcgcggacac gttgcggctg tttatacatt gagcggtgtc
     4621 agcttgatcg cgagcgtgct gctgcaaaat catttttgga cctcctatgt tttcatgtgc
     4681 ttcgcgatac cgggaccgtt cgcggcgatt gcgccgttct gggccatcac tgctgaaacc
     4741 atgccgcgtt acgcgatggg tttggttatc gggttggtga acgccatcgg caacacgggt
     4801 ggattcgcgg ggccatacat caccggctgg ttgaagaaag aatctggcgg catcacgatt
     4861 ccgtttgcgg cgctgggcgt gggaatgttg gttgccgcag gactttgttt cctgctcccc
     4921 cggtcgcgcg tgcagaatgt gactgccgct ctgccagtgg ttgatgcatc ggtttgaaag
     4981 ctctttaagc cacggatgaa acacagattg aacacggatt tgtctgttgc ttaattggcg
     5041 tctcttgccc gtcaatggcc ggttttggaa aacaggggca gcacagtttg atggttttca
     5101 ctttgcttcc gtgtttcatc tgtgtttcgt ccgtggctca atctctcttg tcgagcttcg
     5161 tctggaacgt ccgtttatgg ccggtcgcag gaatggtgat ggtgtaggtt ttcccatcgg
     5221 aagcgacgga tagtttgatg caattcccgg cgcatttgcg ctcaaggtta gcgatgtatt
     5281 catcatcggt attttccatg gcgcctttga agaaactttt gtgaagctgg taacgggctt
     5341 ggatggacga tatcgctttc atggtggcga gcgaattggg attgccgcct ttgtccgcgc
     5401 cgttgtccat cacagaaact gtcggtgaga ggctctgcac gagcaacgga ttattgctca
     5461 agtcggtgcc gtgatggtca acctgataca catccaccgt tccaaccaaa tttaccggac
     5521 aaacaaggcg ctcttccatg ttccaggtca aatcgccgcc gtcgaaaaat cgaaacggcc
     5581 cgaacgaaag cagagtcacg acgctgttgg cattatccga ggtatcgatt tctctcaatt
     5641 ttggatctgc gcaaagtgaa ttggtggttg cgcccttggg aatgtgtcgg gtgaagtttt
     5701 gtttagcagc gagacatcgg agtttgatgc gcgcgacatc cttgtcaggt gattgagcca
     5761 gcgcaatcac atcatcagcg tgaatgataa cgcgtttgcc aaccttcatt tcccgatatg
     5821 gcttgata
//
LOCUS       DASZMM010000013         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4676779, whole genome shotgun sequence.
ACCESSION   DASZMM010000013 DASZMM010000000
VERSION     DASZMM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4676779"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VG754_00280"
     CDS             <1..250
                     /locus_tag="VG754_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17668.1"
                     /translation="PEGGKQLVQLANILLQHGQQEIQRNWFRLAREKFKFKASEEVLK
                     VLPMAEQIKQEDMQREKARIQDIMLKLYGKDLLDEIIE"
     gene            complement(433..606)
                     /locus_tag="VG754_00285"
     CDS             complement(433..606)
                     /locus_tag="VG754_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17669.1"
                     /translation="MTATKLLGRSAVFPTCRIAKIAPDVESGVAPGVAGGILPPAILA
                     TNFVYIFSTFSCI"
     gene            633..2057
                     /gene="gatB"
                     /locus_tag="VG754_00290"
     CDS             633..2057
                     /gene="gatB"
                     /locus_tag="VG754_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWF17670.1"
                     /translation="MEYEAVIGLETHVQLKTNSKMWCGCANEFGAPPNTNVCPVCLGL
                     PGVLPVANDEGLRLTVLTGYLLNCSIPRFAKFDRKNYFYPDAPKNYQITQYDKPSTAN
                     GYVDFEFEGGISRVRITRAHLEEDVGKNFHFERNSGVDFNRAGVPLLEIVSEPDITSP
                     DMAYTYLNALKDILVYGGVSDCDMEKGMVRCDVNISVRPKGQKELGAKIEIKNMNSFS
                     GARKALEYEIPRQIEVLTKGGKLTQETRRWDDVANITETMRTKEQAHDYRYFPEPDLM
                     PFEPTEAWLADVCKRVVELPLARKRRFMDSYQLPAPDAETFVNDVPLGNYFESAIVGA
                     KNPKAVANWIINNLRAKITETQTPLDAVKVKPSDIPELVTLVDSGKISSKIAQDVFAE
                     MFATGDSPGAIVEKKGLAQVSDTGAIERFCDEVIAANPKSAEDFRAGKVAALNFLKGQ
                     VMKLSKGKANPNLVGEILEKKLKS"
     gene            2110..2493
                     /locus_tag="VG754_00295"
     CDS             2110..2493
                     /locus_tag="VG754_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWF17671.1"
                     /translation="MPSKQIIKPAKCASAVGPYNHAVRVGDLLFCAGQIPLDPATCQL
                     VHGDIKAQTERVLENIKAILEDQKLTFANVVKSTVFLTNLADFTAMNEVYAKYFTADF
                     PARSTIQVAALPRAAIIEIEVIAHF"
     gene            2561..>3560
                     /locus_tag="VG754_00300"
     CDS             2561..>3560
                     /locus_tag="VG754_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWF17672.1"
                     /translation="MSSIASLLIGFAVGGALCFAIGWLVGSRRGPGLPPDNRLEAELR
                     QQLTQRETELAKEREQTSKMNAACAAAEAQKQAAENNLSLQKQQHDRAMAEMKVMQEK
                     AIVDLQVAFKALSAEALKQTAPEFLRLAEQSFGKLQEAAKGDLAQRQESIKTLVEPLK
                     QQLETYQTRLQQSETAQSTTLGEVKKQLETLAVQSQSLASETQQFRMVLKSNQARGRW
                     GEETLRRVVEAAGMSAHCDFTEQTQSGDNRPDLVVRLPGERMIIVDAKVPDLDFLNAL
                     DAADTLKRAEALAAHATKLKTTITALAARDYPREFPNALDHVVLFLPAESLFSAALE"
BASE COUNT          876 a          965 c         1013 g          706 t
ORIGIN      
        1 gcccgaggga ggaaagcaat tggtgcagct tgccaacatc ttgctgcaac acggacagca
       61 ggagatccaa cgcaactggt tcaggctggc gcgggagaag ttcaagttca aggcatcgga
      121 ggaggtgctc aaagtcctgc ctatggccga acaaatcaag caggaggaca tgcagcggga
      181 aaaggcgcgg atccaagaca tcatgctcaa attatatggc aaggatttgc tggacgaaat
      241 catcgaatga acctcaacct ttgtaacccc ttgcaatccc gtttactccc acgtgctccc
      301 gctcaatccc gccaaaatta cccacccccc tcccgcccaa gtgcggagtg tggaatacta
      361 gcaagatacg acgctgcacc agtgccggca ggcgtccccg cctgccgtaa agccgggcat
      421 cctgcccggc ggctaaatgc atgaaaaagt tgaaaatatg taaacaaaat ttgtcgcgag
      481 aattgcaggc ggcaagatgc cgcccgccac gccaggcgcg acgcctgact ccacatccgg
      541 agcgatcttg gcgatacggc aggttgggaa cactgcgctg cgtcccagca atttggttgc
      601 ggtcattcgc ctctgggtta atctgttttg gcatggaata cgaagccgtc atcgggctgg
      661 agacacatgt ccagctcaaa acgaactcga agatgtggtg cggttgcgcg aatgaattcg
      721 gcgctccgcc gaacacgaat gtctgcccgg tctgcctcgg tttgcccggc gtgttgccgg
      781 tcgccaatga cgaaggtttg cgcctgacgg tgctgaccgg ctacctactc aattgttcca
      841 tcccgcgctt tgccaagttc gatcgcaaaa attatttcta tccggacgcg ccgaagaatt
      901 accagatcac gcaatacgac aagccctcga cggcgaacgg ttacgtggat tttgaatttg
      961 aaggcggcat ttcgcgtgtg cgcatcaccc gcgcgcatct ggaggaggac gtgggcaaga
     1021 attttcattt cgaacgcaac agcggcgtgg atttcaaccg tgccggcgtg ccgttgcttg
     1081 aaattgtttc tgagccggac atcaccagcc cggacatggc ttacacttac ctcaacgcgc
     1141 tcaaggacat tctcgtttat gggggcgtga gcgattgcga catggaaaag ggaatggtcc
     1201 gttgcgacgt gaacatcagc gttcgtccca agggccagaa ggagcttggc gcaaagatcg
     1261 agatcaagaa catgaacagc ttcagcggcg cacgcaaggc gttggagtat gaaatcccgc
     1321 gccagatcga ggtgttgacg aagggcggca agttgacgca ggagacgcgg cgttgggacg
     1381 atgtcgcgaa catcaccgag acgatgcgca cgaaggagca ggcgcatgat taccgttatt
     1441 ttcctgagcc ggacttgatg cccttcgagc cgaccgaggc gtggctggcg gatgtttgca
     1501 agcgcgtggt ggaattgccg ctggcgcgca agcggcgctt catggacagc tatcagttgc
     1561 ccgcgccgga cgcggagacg ttcgtgaacg atgtgccgct ggggaattat ttcgaatcgg
     1621 ccatcgtcgg cgcgaaaaat cccaaggcgg tcgccaactg gatcatcaac aatttgcgcg
     1681 cgaagatcac cgagacgcaa acgccgttgg acgcggtgaa ggtcaagcca tcggacattc
     1741 ctgagttggt gacgctggtg gacagcggca agatcagcag caagattgcg caggatgttt
     1801 ttgcggagat gttcgccacc ggcgattccc ccggcgcgat cgtcgagaaa aaaggacttg
     1861 cccaggtgag cgacaccggc gcgattgaaa gattctgcga cgaagtcatc gccgccaatc
     1921 ccaagagcgc ggaagatttt cgcgctggca aggtcgccgc gttgaatttt ctcaaaggcc
     1981 aggtgatgaa gctgagcaaa ggcaaggcga acccaaattt ggtcggcgag attttggaaa
     2041 agaaattgaa gagttgaccc ctattcgaga attgttaaac ctcaatccta aactaaaaac
     2101 cattcaaaca tgccatccaa acaaatcatc aaacccgcaa agtgcgcctc agccgtcggt
     2161 ccatataatc acgccgtgcg cgtgggcgac ctgctgttct gcgccggcca gattccgctc
     2221 gaccccgcca cctgccagct tgtccacggc gacatcaagg cccagaccga acgcgtattg
     2281 gaaaacatca aggccattct ggaagaccag aaactcacct tcgctaatgt ggtcaagagc
     2341 acggtgtttc tcacaaacct cgccgacttt accgcgatga atgaagttta cgcgaaatat
     2401 ttcaccgctg atttccccgc gcgctcgacg attcaagtcg ccgccctgcc ccgcgccgcg
     2461 attatcgaga tcgaagtcat cgcgcatttc taaatttacg atttacgatg gacgcgcgat
     2521 acagccgagc atcgcggaaa tcgtaaatcg attatcgtaa atgtcatcaa ttgccagttt
     2581 attgatcggt tttgctgttg gaggagcttt gtgcttcgcg atcggctggc tggttggctc
     2641 gcgccgagga cctggtttgc cacccgataa ccggctggaa gcggagttgc gccaacagtt
     2701 gacgcagcgc gaaacagagt tggccaagga acgggaacaa acttccaaga tgaatgcggc
     2761 ttgcgcggct gcggaagctc aaaagcaagc tgcggaaaat aatctctccc tgcaaaagca
     2821 acagcatgat cgggcgatgg cagagatgaa ggtcatgcag gaaaaagcca ttgttgactt
     2881 gcaagttgcg ttcaaggcgc tcagcgctga ggctctcaaa caaaccgcgc ctgagtttct
     2941 gcggctcgcc gagcaaagtt tcggcaaact tcaagaagca gcaaaggggg atctcgcaca
     3001 acggcaggaa tccatcaaga ctttggtgga accgctcaag caacagttgg agacttatca
     3061 aacgcgattg caacagagcg aaaccgcgca atccaccacc ttgggcgaag taaaaaagca
     3121 gttggaaacg ttggcggttc aaagtcagtc gctcgccagc gaaacgcagc aattccggat
     3181 ggtgctcaaa tcgaaccagg cgcgcggtcg ctggggcgag gagacattac ggcgcgttgt
     3241 cgaggcggcc ggcatgagcg cgcattgcga ttttactgaa caaacccagt cgggcgacaa
     3301 tcgccctgat ttggttgtga gattgcccgg cgagcggatg attatcgtgg acgccaaagt
     3361 tcccgacctc gattttctca atgcgcttga cgctgccgac acgctcaaac gcgcggaagc
     3421 gcttgctgcc cacgccacaa aattaaaaac caccatcacg gcgcttgcgg cgcgcgatta
     3481 tcccagggaa ttcccgaatg cactggatca tgtggttttg tttttgccgg cggaatcgct
     3541 tttcagcgcg gcactggaag
//
LOCUS       DASZMM010000014         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2479335, whole genome shotgun sequence.
ACCESSION   DASZMM010000014 DASZMM010000000
VERSION     DASZMM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479335"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="rlmN"
                     /locus_tag="VG754_00305"
     CDS             complement(<1..1008)
                     /gene="rlmN"
                     /locus_tag="VG754_00305"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWF17673.1"
                     /translation="MLTDIRSQIIEALQARFQEWKQPVYRVPQLLEWLYGRRVTGWDA
                     MTNLPKALRELLRENYSLRALELVRKQGSRDTTQKFLWRLDDHSLIESVLIPANPALY
                     GEPSDRHTLCVSTQVGCAYGCKFCASGLEGWKRNLRVEEIVEQVLATERWNEAELTKG
                     DNPELGTRNPERLVNNLVIMGMGEPLANYDNLLAALKILNAPWGGGIGARKITISTSG
                     LAPQIRKLADEPLQFRLAISLHGATDQTRNKIMPVNRKYPLSELTSACEYYQQKKERM
                     ITLEYILIAGVNDSLSEIKPLAQLARRLHAKVNLIPYNKVEDLSWERPPEAVQEAFLS
                     AL"
     gene            1164..2006
                     /locus_tag="VG754_00310"
     CDS             1164..2006
                     /locus_tag="VG754_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWF17674.1"
                     /translation="MNFLNKKIAPLLAASMLLALAHPGAIDGQTYYFSTPVSGSQEVP
                     FADGNNPNTFGFPGGNGNGNGKYPGFSWNASFGTLNGTIVYNSTADTIDLSGSLAVNP
                     ATYNSSFTDNQMVHGNLVSANVSVSSCVANANNGFFSFDSGAVQPVKGQDAEWEISMQ
                     VPVMGTYSFFTGGQTYNGSFNCTLTLPTLINLQSIDPSSLTFSQYVENGRDNAGGFLA
                     HVDAANGAQMDLSSLDPSDGSTYGTWQLNNVSATAPEPGTLALLGCGLAGLALLRRGK
                     TKSC"
     gene            complement(2030..>3997)
                     /gene="glgP"
                     /locus_tag="VG754_00315"
     CDS             complement(2030..>3997)
                     /gene="glgP"
                     /locus_tag="VG754_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWF17675.1"
                     /translation="RQVLKAFDAYLNDQNTWTATHAPSLLKNPVAYFSAEFGFHETLP
                     IAAGGLGILAGDHAKSASDLGLGFVGISLFYREGYFQQAINQDNWQTEYYTLLNPKNL
                     PIEPVLDAKGEPLICSVEITMNQVFFQVWRVNVGRNPVYLLDTNRPENPQHFRDLTLR
                     VYGGDTTTRIMQEIVLGIGGVRLLRALGVEPSVYHMNEGHAAFLTLELIREKMAAGKS
                     YEEALALTRQQCIFTTHTPVEAGHDRFTPELMDYALHKYAAQLRLPAKELLALGRVDP
                     KNAGEPFCMTVLALKASRAANGVSELHGQVSRHMWKSLYPNLPEDKVPIGHITNGIHL
                     LGWMKGPVRSFWRRRLTDGNQECPAEFTDTARFWKGAADTGWDKAINSPDFWQKMLDP
                     NFISDEELWALRYKLRRELIEFARRRLAAQGQRLAQVDFISFDQLLNPDALTIGFARR
                     FATYKRAPLIFQQLENIVRLARDHERPIQFIFAGKAHPRDDDGKRYIQNIVHLSKYSD
                     MQGRLVFIENYDVHVARQMVSGCDVWLNNPRRPLEASGTSGMKAGCHGCLNLSILDGW
                     WREGYDGTNGFAIGADAHAASVEEQDHQDSANLYKTLTEQVIPTFFERDAQGLPRKWI
                     QMIRRAMATLVPQFSTRRMVKEYTEKYYLTK"
BASE COUNT          837 a         1110 c         1152 g          898 t
ORIGIN      
        1 cagcgcggac aaaaaagctt cctgcacagc ttcaggaggg cgttcccacg aaaggtcttc
       61 aactttgttg taggggatca aattcacttt cgcatgcaga cggcgcgcga gttgcgccag
      121 cggttttatc tcgctcaaac tgtcgttgac cccggcgatc aaaatatatt ccagcgtgat
      181 catccgctct tttttctgct gataatattc acaagcggaa gtgagttcgg agagcggata
      241 tttccggttc acgggcatga ttttgttgcg agtttggtca gtagctccgt ggagcgagat
      301 ggcgagccgg aattgcagcg gctcatcagc gagcttgcgg atttgcggcg caagaccgct
      361 cgttgaaatc gtgattttgc gcgcgccgat gccaccgccc caaggcgcgt tcaaaatttt
      421 caacgcggcg agcaggttgt cgtaattggc aagcggttcg cccatgccca tgatgacgag
      481 gttattgacc aagcgttccg gattccgggt tccgagttcc gggttgtcac ccttggttag
      541 ttccgcttcg ttccaacgct ccgtggcgag gacttgctcg acgatttcct ccacgcgcaa
      601 atttcgtttc caaccttcca ggccgctcgc gcaaaacttg cagccatacg cgcaacccac
      661 ctgcgtcgag acgcagaggg tatggcggtc gctcggctcg ccgtaaagcg ccgggttcgc
      721 gggaatcagc acgctctcga tgagggaatg gtcgtcgagg cgccagagaa atttctgcgt
      781 ggtatcgcgg gaaccttgtt tgcggacaag ctcgagcgcg cgcagggaat aattttcgcg
      841 caacagttcg cgcaaggctt tgggcaggtt tgtcatggcg tcccagcccg ttacgcgccg
      901 gccataaagc cattcgagca attgcgggac gcgatacacc ggctgtttcc attcctggaa
      961 acgcgcctgc aatgcctcga tgatttgcga tctgatgtct gtcagcacga ggcaacttaa
     1021 ccgaaacggg ccatcagaaa agacgaaaag tagggtgaaa tccgtttgga gtatgtataa
     1081 ctacggatag ggagaaacgc gcaatgagct tgacgccacg ggcctgctga aaaactatat
     1141 ttgagaaaat caatcggaga taaatgaact ttctgaacaa gaaaatagcg cctcttttgg
     1201 cggcttccat gctccttgcg ctcgcccatc ccggggcgat tgacggccag acttattatt
     1261 tcagcactcc tgtgagcggc tcccaggaag ttccattcgc cgatggaaac aatccgaaca
     1321 cttttggttt tcccggcggc aacggcaatg gcaacggcaa atatcccggt tttagctgga
     1381 acgcctcttt cggcacgctg aacgggacca tcgtttataa ttccaccgca gacaccattg
     1441 acctcagcgg gagcctggcc gtcaatcctg cgacgtataa ctcttcgttc accgacaacc
     1501 agatggtgca cggcaatttg gtgtcggcca atgtgtccgt cagttcctgc gtggcaaatg
     1561 ccaacaatgg ctttttctca tttgattccg gcgcggttca gcctgtcaaa ggccaagatg
     1621 cggagtggga gataagcatg caggtgcctg tcatgggaac atattcgttc ttcaccggcg
     1681 gccaaactta caacggttcg ttcaattgca cgttgacttt gcccaccctt ataaaccttc
     1741 aaagcatcga tccctccagc ctgactttta gccagtatgt tgagaatggc cgtgataacg
     1801 cgggcggttt tctcgcgcat gtggacgccg ccaacggtgc gcaaatggat ctctcgagct
     1861 tggacccttc ggacgggagc acctacggca cttggcaatt gaacaatgtc agcgccaccg
     1921 cgcccgagcc gggcactctg gcccttctcg gctgcggact tgccgggctt gcgctgctgc
     1981 gccgcggcaa gacaaagagc tgctgaagtt tcgcccaatg cggcgcggct tacttcgtca
     2041 gataatactt ctcagtatat tccttcacca tgcggcgcgt gctgaattgc ggcaccagcg
     2101 tggccatggc gcggcgtatc atctggatcc atttgcgcgg caatccttgg gcatcgcgct
     2161 cgaagaaggt cgggatgacc tgttcagtaa gcgttttgta aaggttcgcg ctgtcctggt
     2221 ggtcttgttc ttcaacggaa gcggcgtggg cgtcagcgcc aatggcaaag ccgttcgtgc
     2281 cgtcgtaacc ttcgcgccac cagccatcca aaatgctcag attaaggcaa ccgtggcagc
     2341 cggctttcat gccgctggtg ccggaagctt ccaatggcct gcgcggattg ttcaaccaga
     2401 catcgcagcc ggaaaccatc tggcgcgcca cgtgaacatc gtaattctcg atgaagacca
     2461 gacgcccttg catgtcgctg tatttgctga ggtgaacgat gttctggatg taacgcttac
     2521 cgtcgtcgtc gcgcggatgc gccttgccgg caaaaatgaa ctggatgggc cgttcatggt
     2581 cgcgagcgag gcgcacgatg ttttcaagtt gctggaaaat cagcggcgcg cgtttgtagg
     2641 tcgcgaaacg ccgcgcaaaa ccgatggtca aggcgtccgg attcaacagt tgatcgaatg
     2701 agatgaaatc cacctgcgcg aggcgttgtc cctgcgccgc aaggcggcgt cgcgcaaatt
     2761 caatcagttc acgccgcaat ttgtatcgca gcgcccatag ctcctcatcc gaaatgaaat
     2821 tgggatcgag catcttctgc cagaaatccg gcgagttgat ggccttgtcc cagccggtgt
     2881 cggcagcgcc cttccaaaaa cgcgcggtat cggtgaattc ggccgggcat tcctgattgc
     2941 cgtccgtgag cctgcgccgc cagaaactgc ggaccggccc tttcatccag ccgagcagat
     3001 ggatgccgtt cgtgatatgc ccgatgggca ctttgtcttc gggaagattc ggataaagcg
     3061 atttccacat gtggcggctg acctgcccgt gcaattcact gacgccgttg gcggcgcgcg
     3121 acgctttgag cgcaagcacc gtcatgcaaa acggttcgcc ggcatttttc gggtccacgc
     3181 ggccgagcgc gagtaattcc ttcgcgggca accgaagttg cgcggcatat ttgtgcagcg
     3241 cgtaatccat caactccggc gtgaaacggt cgtgaccggc ttcgacgggc gtgtgcgtgg
     3301 tgaaaatgca ttgctggcgc gtcagcgcga gggcttcctc gtaagacttg cccgcggcca
     3361 ttttctcgcg tatcaattcc agcgtgagaa aagccgcatg gccttcgttc atgtgataga
     3421 ctgacggttc gactcccagc gcgcgcaaga ggcggacgcc gccgatgccc aggacgattt
     3481 cctgcatgat gcgcgtggtg gtgtcgccgc cgtaaacgcg cagggtcagg tcgcggaagt
     3541 gttgcgggtt ttccgggcgg ttggtgtcga gaagatagac cgggttgcgc ccgacgttca
     3601 cgcgccaaac ctggaagaac acctggttca tggtgatctc caccgagcag atgagcggtt
     3661 cgcccttcgc gtccaatacc ggttcgatgg gcagattttt cggattgagc agcgtgtaat
     3721 attccgtctg ccagttgtcc tgattgatcg cctgctgaaa atatccttca cgatagaaca
     3781 ggctgatgcc cacaaagccc aaacccaaat cactcgcgga tttggcgtga tcaccggcga
     3841 gaatgccaag gccgccagcg gcgatgggca gcgtttcgtg aaaaccgaac tcggcggaaa
     3901 agtatgcgac tggatttttc agcaaggacg gcgcgtgcgt tgcggtccag gtgttctgat
     3961 cgttgagata agcatcgaag gctttcaaca cctgccg
//
LOCUS       DASZMM010000015         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3304810, whole genome shotgun sequence.
ACCESSION   DASZMM010000015 DASZMM010000000
VERSION     DASZMM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304810"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VG754_00320"
     CDS             complement(<1..974)
                     /locus_tag="VG754_00320"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWF17676.1"
                     /translation="MGTKLWKQIIAGVAMFCLAFALGAAPTNETIFTHGVVASIDPIA
                     TDAGVNVLKSGGNAVDAAIVVGLTLGVVDTPDSGIGGGCFMLIHLADGRNIALDGRET
                     APAAATRDMFVRNGKGDTELSQTGPLASGVPGELAAFDFASRNYGKKSLRDLILPAAE
                     IAEKGFTVKSSYARRLEAEAKGMAAFEASRVVFFKDGEPVKAGAILKQHDLAETYRQI
                     GTHGADWFYRGPFAQAVEKWMKENDGLLTAADFANYHAQLRDPIESSYRGYRLVSFPP
                     PSSGGVHVVEMLNILENFEMKKLDEVSRLHLIADAMKLAFADRAYWLG"
     gene            1076..2062
                     /locus_tag="VG754_00325"
     CDS             1076..2062
                     /locus_tag="VG754_00325"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HWF17677.1"
                     /translation="MKVAQFGLGPIGIETLKLAATKPWVEIVGGIDIDPAKVGKSLSE
                     LTGLPELASARVYRSLEELLAVARPDLIFHTSVSKIKTAVEQIEPMAQKGISVISSCE
                     ELLFPKLRDPALAAQLNATCVRHGARVLGTGVNPGFVMDVLPVCLTGVCRSVKAVHVQ
                     RVVNATTRRGPLQKKIGSGLAPAEFERLFQEGKAGHAGLQESAALIAHCLGWKISKLT
                     ETCKAVVADHDIRTPHVEVMAGQTCGLHQHGEATIGGRVCITLDLKMYLDAPNPHDSC
                     QIDGEPPLTMTIAGGVAGDSATVSSLVNAAPRVLKAPPGLLLMTDIAVPSYA"
     gene            complement(2440..>3598)
                     /locus_tag="VG754_00330"
     CDS             complement(2440..>3598)
                     /locus_tag="VG754_00330"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HWF17678.1"
                     /translation="NPRSPWVTFGTSASGTPYQGPFGKSQAGLNVGQIFLGWHPEDWV
                     NITVGKMAMPLYTTPMVWDTDYNPEGLAEHFKYKAGDFELFANLGQFLYEDTNPNKAS
                     QGYFGVGNLYPSPSGGAAGPVFLLAWQLGAKYNINKDMSFQVAPVVYNYTGHGANTSQ
                     SASLIEPGFSGVFVGQGATLGAGGKPAAGWSGFPNGAQDGFTANQTGINDLLVLEFPW
                     EFNFKICQFNARVFGDYAQNLEGAERARAAAAAANSLAGVQKISSAQTQDDKAYQIGL
                     AVGNNLGTAYGVATPKHGWEFRTYWQHVEQYALDPNLIDSDFFEGRGNLEGVYAAFAY
                     GFTDNVMGTFRYGHAHRINGLLGTGGSNQDIPQMNPIEHYDIIQLDLTMRF"
BASE COUNT          771 a         1043 c         1021 g          763 t
ORIGIN      
        1 tcgcccagcc agtaagcgcg gtcggcgaat gccagtttca tggcgtcagc gatgagatgc
       61 aggcggctga cttcatccaa ttttttcatc tcgaaatttt cgaggatgtt gagcatctcg
      121 acgacatgca cgccgccgga actgggcggc ggaaaggaaa ccaaccgata gccgcgataa
      181 ctcgattcaa tcgggtcgcg caattgcgca tgataattgg caaagtccgc ggcggtgagc
      241 agaccgtcgt tttccttcat ccatttttcg accgcttgtg cgaaaggtcc gcgataaaac
      301 caatccgcac cgtgcgtccc aatctgtcga taggtctcgg cgaggtcgtg ctgtttcaaa
      361 atggcaccgg ctttcaccgg ttcgccgtcc ttgaagaaca ccacgcggga agcttcgaat
      421 gcggccatcc cttttgcttc ggcctccaat cgtcgcgcat aacttgattt aacagtgaaa
      481 cctttttccg caatttccgc cgcgggcagg atgaggtcgc gcaaagattt ttttccataa
      541 ttgcgcgagg cgaaatcaaa agcggccagt tcgcccggaa caccgctggc aagagggccg
      601 gtctggctca attccgtgtc gcccttgcca ttgcgaacga acatgtcgcg cgtcgcggcg
      661 gcaggcgctg tttcgcgtcc atcgagcgcg atgttccggc cgtcggcgag atggatgagc
      721 atgaaacagc cgccgccgat gcccgaatcg ggcgtatcga ccacgccgag agtgaggccg
      781 accacgatgg cggcatccac ggcgttgccg ccggacttga ggacattcac gccagcgtcg
      841 gtcgcgatgg gatctatgga ggcaacgacg ccatgggtga aaattgtttc gttcgtcggg
      901 gcagctccaa gcgcgaatgc caagcaaaac attgcgacac ccgcaattat ttgtttccaa
      961 agctttgtgc ccataagagg attttgaact gcgccatccg tgataaacct tcagggacgt
     1021 ttccgccatc caaaaaacct acacatttaa aggcaatctt gttaaaaaaa cggaaatgaa
     1081 agttgcgcaa ttcggactcg gccccatcgg cattgaaact ttgaagctcg ccgccaccaa
     1141 accgtgggtg gaaatcgtcg gcggcattga cattgatccg gcgaaggtcg gcaaaagcct
     1201 cagtgaactc accggtttgc ctgagcttgc cagcgcgcgc gtttaccgtt cgctcgaaga
     1261 attgctcgcg gttgcgcggc ccgacttgat ttttcacacc tccgtttcaa aaatcaaaac
     1321 cgccgtcgaa caaatcgagc ccatggcgca gaaaggcatc agcgtgattt cgtcctgcga
     1381 ggaattgctc tttcccaaat tgcgcgaccc ggcgcttgcc gcgcagctta atgccacttg
     1441 cgtccgacac ggcgcgcgcg tgctcggcac gggcgtcaat cccggatttg tcatggatgt
     1501 gctgccggtt tgcttgacgg gagtttgccg ctcggtaaag gccgtgcatg tgcaacgcgt
     1561 cgtcaatgcc accacgcgac gcgggccgtt gcagaaaaaa atcggcagcg gccttgcgcc
     1621 ggcggaattc gagcgattgt tccaggaagg caaggctggc catgcgggat tgcaggaatc
     1681 cgccgcgctc atcgcccatt gtctcggctg gaaaatttcc aaactcaccg agacttgcaa
     1741 ggcagtggtt gccgatcacg acatccgcac gccgcatgtc gaggtgatgg ccggccagac
     1801 ctgcggcttg catcagcatg gcgaagcgac catcggcggc agggtgtgca tcaccctcga
     1861 tttgaaaatg tatctcgacg cgccgaaccc gcacgacagt tgccagattg atggcgaacc
     1921 gccgttgacc atgaccatcg cgggcggagt ggccggcgat tcggcgacag tctcgtcgtt
     1981 ggtgaatgcc gcgccgcgag ttttgaaagc gccgccggga ctgttgctga tgaccgacat
     2041 cgccgtgccg agctacgcgt agaacttttc tcagtcggaa tcctgccgat gaaaaagggg
     2101 agcgcgcgcg tctcgcgtgc tgtccgagcc gtcccggcga ggacttcaac gaactgactt
     2161 ttcaacatcg gtggtcagaa gaaaatcagt attacgaagt gttcggcggg acgccgaaca
     2221 ctgcacgccg gaggcgtgcg ctcccccttc aactgaatcg tcacggccaa ggagtggttg
     2281 aaacattttc tgcggccaga cccgtcttca cactttgttg gccatgactt tatttcaact
     2341 gctataaacg aagaacccag gaacagaggt ggaatcattc ctccattcct gagttcgttt
     2401 tggttctttc accgcgtgat gcgatgaact tgggccggct cagaatcgca tcgtcaaatc
     2461 caactggatg atgtcgtaat gctcgatggg attcatctgc gggatgtcct ggttgcttcc
     2521 gccggtgccc agcaagccgt tgatgcgatg cgcatggccg taacgaaacg ttcccatcac
     2581 attatcggtg aacccatagg cgaacgcggc gtaaacgcct tccaagttgc cacggccttc
     2641 aaagaagtcc gagtcgatca agttggggtc gagcgcatat tgttcgacat gctgccagta
     2701 agtcctgaat tcccatccgt gtttcggcgt ggcaacgccg taagcggtcc caagattatt
     2761 gccgactgcc aggccgatct gatacgcctt gtcgtcctgc gtttgcgcgg aagaaatttt
     2821 ttgcacgccc gcaagcgaat tggccgccgc ggcagcagcc cgcgcgcgct ccgcgccttc
     2881 caggttttgc gcgtaatcgc cgaagacacg ggcattgaac tggcaaatct tgaaattgaa
     2941 ttcccacggg aactcaagga ccagcaggtc attgatgccg gtttgatttg cggtgaagcc
     3001 gtcctgtgcg ccattgggaa aaccgctcca acctgccgca ggtttgccgc ccgcgccaag
     3061 cgtcgcgccc tggccgacga agaccccgga aaatcccggc tcgatcaacg aggcgctttg
     3121 cgaggtgttc gcgccgtgcc cggtgtaatt ataaacgacc ggggcgacct ggaacgacat
     3181 gtccttgttt atgttatatt tcgcgcccaa ctgccatgcc agcaggaaga ccggtcccgc
     3241 ggcgccgccg ctcggcgagg gatacaaatt gccgacgcca aaataacctt gagacgcctt
     3301 gttcggattc gtgtcctcgt aaaggaactg gcccagattg gcgaaaagct cgaagtcacc
     3361 cgccttgtat ttgaaatgct cggcaagacc ttccggattg taatctgtgt cccaaaccat
     3421 cggcgtggtg taaagcggca tggccatctt gccgacggtg atgttcaccc aatcttccgg
     3481 atgccagccg agaaaaattt gcccgacgtt caggcccgcc tgcgatttgc cgaacggccc
     3541 ttgatacggc gtgccgctgg ccgatgtgcc gaacgtcacc cacggcgaac gcggattg
//
LOCUS       DASZMM010000016        16389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2479483, whole genome shotgun sequence.
ACCESSION   DASZMM010000016 DASZMM010000000
VERSION     DASZMM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479483"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..404)
                     /locus_tag="VG754_00335"
     CDS             complement(105..404)
                     /locus_tag="VG754_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HWF17679.1"
                     /translation="MDKPPSQCWIDFLEEEDLAFIKRFILASGSLKELAAAYNVTYPT
                     LRLRLDRLIAKITVLDNQKIEDDYERLLRAMFAEGKLDAVTFKPLLQTYQKKGKP"
     gene            697..1479
                     /locus_tag="VG754_00340"
     CDS             697..1479
                     /locus_tag="VG754_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF17680.1"
                     /translation="MRKFALKAFGVGDGWPCADRNHASFLYRLGDVSILVDCGDCISR
                     SYKASGLDYNLIDRIFISHLHSDHVAGFFMLMQGFWLEQRKKDLPVSMPADGIKPISE
                     LLNAGLIFKELLQFRLRFEALRVGKPVMTKDVRVTPYPSTHLEQLRRSFQKRYPQDYA
                     AYCFLIERGKLRIGHSADIGCPEDLEPLLKKPLDLLVCELAHFHPEDLFLYLQGRDIK
                     KILFMHVGRSYWEDLKKTQRLAAKMMPDIPFGFLHDGQEVPL"
     gene            2555..4138
                     /gene="murJ"
                     /locus_tag="VG754_00345"
     CDS             2555..4138
                     /gene="murJ"
                     /locus_tag="VG754_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWF17681.1"
                     /translation="MLKSSGALAAATMTSRVLGMVREIVYYAFMGVGMVNGAFTLAFM
                     IPNLFRRLLGEGALTASFIPIFKEKERTSTEAEMWRAANAVISGMMAVASVITVLAIL
                     GISVALATTEFPEKTELMLRLLRIMFPYMLFICLTAIFMGMLNARGHFFIPAMGAVVM
                     NVVMISAVYFLAPRMGATLEQKIFGLAIGVLVAGVAQAIVQLPSLRGEGFRYYWVSPW
                     NDPTVRRVMRQMIPGTIGIAAFQINVLTTQSVAFWVDPSIVASFQGAVRLMELPQGVF
                     GISLATYLLPTLAGLAADKKYDEYRKTLNQGIDHLVFINLLATILLFFLAEPIIRLLF
                     EHGKFIAEDTPKVTWALVCLSPGLLAFSLVNILARAFYALGDTKTPMKISIFCLVINL
                     IFALVLIRKFQQGGLGMANSLSACFNVSLLFYALRKKLGKLEFGALRQMLWPIFVAGI
                     FAALTAWATWHQWDKTFGHFGLARRLGAVFIPMTLASLVYWLVALLFRVPPATELGGL
                     VLQQLPLLSKGKSLKTIEK"
     gene            complement(4483..4558)
                     /locus_tag="VG754_00350"
     tRNA            complement(4483..4558)
                     /locus_tag="VG754_00350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4522..4524),aa:Arg,seq:cct)
     gene            complement(4693..7467)
                     /locus_tag="VG754_00355"
     CDS             complement(4693..7467)
                     /locus_tag="VG754_00355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWF17682.1"
                     /translation="MRKMPKADLRIKLPPVHDWRTTDADEINKRRQRAREESFVITNT
                     DARHPIFSNFRVKSASGLTYSVEIRGLNGPQCACDCVDFRTNGLALCKHTEAVLLYLQ
                     ARHKKHFKSAQTFGSNRIEVAVDAAANILCVPNGCSKLPYAVSKWFDADGRLLNDSPE
                     TAVDALRQLRESDHPQIRISQEVGPWLENHRRVGERRQLRHEYELKVQSGEWPAHETT
                     VPLFPYQREGMLHLAFTERALLADEMGLGKTIQAIAACALLHRLGQARRVLVVTPASL
                     KTEWEEQIRKFTTLDYQMVFGGKIRRLKAYDQTPVTPFFTIVNYEQMVADTLDVNARL
                     APDIVVLDEAQRIKNWSTKTAQAVKRLRSRYAFILTGTPIENRIDELHSLMDFLNPAV
                     LGPLFRFNRNFYELDERGRPIGCRNLDLLHERIKPFMLRRRKVEVETELPERTDHNHF
                     VPLHDLQKQNYAVHEQQVMKLVNLALRRPLTKQEQEKLQRELAMMRMICDTNYILDPE
                     DRTCPKLGELGKLLEECGENDAKVIIFSEWERMLELVRDLCEQRDLGHAWHTGSVPQQ
                     RRRAEINSFKDDPLCRVFLSTDCGATGLNLQNASVVINCDLPWNPAKLEQRIARAWRK
                     HQTRPVTVFNLISENTIEHRMLETLALKQSVAMSVLDKPGEVKEIKLRSGRQAFVERL
                     QQLVTPAKLTSGHPPSRTPSPINQKKLPADPAVAFAQLASERINGSLIRCEERYPQTG
                     SHTMLVVVVEREPAQWRETLNSLHADLFGPGKTDPLAPVKLEVIDRATDEAIQRLIAA
                     GLINKTTRATRPLFPVSDITESASLSTEELARAKLLRERATRKLKMARVLGEGGFGDE
                     ARPALLDAMLGIACALAVERRLPEPAELKDVLQPPFSNCWANALPTLKMYLDETAADW
                     KPVSECLGVL"
     gene            7628..8548
                     /locus_tag="VG754_00360"
     CDS             7628..8548
                     /locus_tag="VG754_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17683.1"
                     /translation="MPFSYAELEQFYEKLVTRARSRGIACAITSGMACVAFGVSEATK
                     DCDLLCTPDSADDLLGLLGETTLGGHLPSYRGNLTPPLDARWFRGGWTSHFMWPPAES
                     GAYLDIFGVAPRGSSPWEAEIQGFYASPHTVAEMKRTNRDKDWPFVTALGARMIEMGD
                     ERGWLHLYDEKLLRAFSETKRPAALIKRRPVLELAANNDPKLRPALRAEIEYWHELDR
                     VRMNIYENAVRRYMVEVRKSHLPPSAGLPVQHDLRVRCAEKYLPENPMRDFGVTRMIS
                     EAREALSQIINPAAMEWLPDVHEHFTLLTA"
     gene            8545..8766
                     /locus_tag="VG754_00365"
     CDS             8545..8766
                     /locus_tag="VG754_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17684.1"
                     /translation="MKNTGRQKKKNTPIMGRRWAVAIYDFHVRAFGEEMARVNRLPLA
                     ARRRYIGGMVDHAIQKGVKSTKPALGVSP"
     gene            complement(8756..11650)
                     /locus_tag="VG754_00370"
     CDS             complement(8756..11650)
                     /locus_tag="VG754_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWF17685.1"
                     /translation="METIDQNSGNRACSLMPNAVSLNPSIPTVTNNSSPAAKIELFRS
                     LFRGREDVYPRRFESRKTGKAGYAPACANEWVRGVCEKPRIKCTNCPNRRFLQITDEV
                     VGWHLSGRDSNGFDFVMGAYAMLQDETCFFLAVDFDDEDWQRDAAAFLETCRQIDVPA
                     ALERSRSGNGGHIWLFFEEAIPAILARKLGSHVLTETMEDRPEIGLNSYDRLFPNQDT
                     LPRGGFGNLIALPLQKQARQRGNTVFLNEKFNPHTDQWSFLASVSRMSRTQVEVLVRN
                     AESKGRIVGVRVAAPEEDDDDPWTAPPSRRQKEPPISGSLPEKLELILGDQIYIGKEG
                     LVPGLRNRLLRLAAFQNPEFYRAQAMRLSTYDKPRIIACAEDHPKHFAVPRGCLDDVQ
                     KTLEALRIQPVLRDERFGGVSLGVSFCGILRPDQQVAVEALLRHDTGVLAATTAFGKT
                     VVAAWLIAQRGVNTLILVHRQQLLEQWIERLSTFLNLPAKTIGRLGGGRKKLTGTLDV
                     ALMQSLIHKGVVDDRMGDYGHLIVDECHHLSARSFEMVARRAKAKFVTGLSATVGRKD
                     GHHPIIFMQCGPVRHRVNAKTQAAARPFHHVLVRPTAFRASAASDPDPRAEFHQLYEA
                     LTNDEKRNQMICADVVSAVNDGRSPLVLTERIEHLQQLAQVLSPDIQHLILLQGGQSR
                     KELNNARTLLAELPATSPRVILATGKYIGEGFDDSRLDTLFLALPVSWRGTIAQYVGR
                     LHRLHESKREVRVYDYADLNVSMLSRMFDKRCRGYESLGYTILLPGSALPGWPAEIPL
                     PVDPEWKKDYAASVRRLIRDGVDVPLAHLFVHVAKSPAVDVQGEDRARSASEAFLFRR
                     LETLAETAGRFRLNEKLPISFDGSGKMEVDFLCAEAGVVIELDGAQHFADAEAYRRDR
                     RKDALLQRNGYFVLRFLAEDTGKHLDHILDTILATLVHRENSKLCRISMD"
     gene            complement(11861..13942)
                     /locus_tag="VG754_00375"
     CDS             complement(11861..13942)
                     /locus_tag="VG754_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17686.1"
                     /translation="MKTRLFHCFLAVCFGMLLAPSIIAQANMRREGALTLVQTVDDNK
                     GFYSAAIDPTNGFAYFAAKYVYKVNITTPLPTQVGAGVSLGRLAFSAVMDSSAGCSYY
                     AVGTSIYQILANGTNAPQLGAIMSSPFGSSTFLTQLLIDTTDPANHYLYAMTETGNAS
                     STLYKLALNQFPNTSAIIGSAVTTAQQPPLGYGVIDLTNRCAYYGTFVPTTLPPYVAK
                     FSLGAGSNAPINYGGVSLDSSTNRSLGALVLDIANGYGYCDSDGTDLLFGHGRVYKWA
                     LNGTNSPTLVSYTDMHTNEGYAHVAVIKPDRGLLYFSSDLSYPAYVYRFRLPPGTNAP
                     VETGSLPLLPSTYTNVPAWGYNPTNSTNWGEVFARSIAYDPVRDFVYIGRDDADGQVQ
                     PYTDQIVKLALDRDEMVLTLTEDIANLNNAISYRESFEFYTNGYSLVGTNGWFGEDSA
                     MGIISTNADTYTNIFPIPGAHQQMLQVDGAITNRFRQSPDTNVWVDMILQAKYWTDPL
                     MLTFSNAPFALCITTNGHLAVWNRTNPPALGNGWTELADTSIASNQFFRATVEANYNR
                     DANGIFYYRVWVNGVPSTNPQTWYAAADTNENYFGDLVARGRFAMDDLVVTLPTPMIS
                     KIVRNPNGSINLQCGGMPGMSHRILATSSLSPAIWQSVSTNLAGADGSWQFVDTNAAN
                     LPERFYRASLP"
     gene            complement(14096..16204)
                     /locus_tag="VG754_00380"
     CDS             complement(14096..16204)
                     /locus_tag="VG754_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWF17687.1"
                     /translation="MKTPPMLVGAALLFWGWQTEFFIVATVLAVVLEGSRYVRLRWEM
                     TTEDFSRIWTFCTLLLVACLAYAFSASNGPANLRGIFSSAGFMAQNTIATVSTRTAVL
                     VIRWLPLVYFFFIAAQAFSNQEGIPMIVVSSILRRRWKKLKKLTQPMPPMRSMNMAYP
                     YFILCLFAACTHPQPSSGENFFFGLCGLLMWGLWPFRSRRFSTIVWAGAMALAMSLGF
                     GGQRGLVQLQRAIVQYNPQWLADLVDQGFDPIESRTEIGQIGRIKTSHKIVLRLKTMN
                     DEMPPAYLREAAYRQYKSGVWYSGSSKDDFTDVNDQPRNSGNYPLFETKNPLRTLNIA
                     CYLAGGKGLLPLPTETARLEQLQAIFVQKNHVGTVHAQGPGLVIFDALYGYGSAMDYP
                     PDNSENSEDLSIPEKERSAVEHVVSELHLDGLDDDKKLLAISHFFGAQFTYSLWQEPT
                     RVMDTNMTPLGRFLLQTHKGHCEYFATATVLLLRQLHIPARYTVGYAVHEKSGSGYVV
                     RQNDAHAWCRVYNERRGRWENFDTTPGIWMQAEREQRSSFQFISDWWSRAVFEFSKFR
                     WGQSRLRQLLPWVLVPVLALMLFQILFRSNARRARKKSKQKSIPMHRPGMDSEFYELE
                     RALVQRGAARRPGEPLSDWLRRTSAAPELSRLNEPLEEILRLHYRYRFDPKGLDMDHR
                     ERLRREVAACMIFMASGRKA"
     gene            complement(16201..>16389)
                     /locus_tag="VG754_00385"
     CDS             complement(16201..>16389)
                     /locus_tag="VG754_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWF17688.1"
                     /translation="LEWDESRRNFVKKLRFSGVPVVVVVIVESLPDKPLEPGPMADDP
                     AHFHTLVLGRIEEGLGQL"
BASE COUNT         3719 a         4345 c         4505 g         3820 t
ORIGIN      
        1 tgtcggaatc gttttcaaat cctgccattt gtattcgccc agtatttcct ggctccatgc
       61 ggcccggcag atgagaatca aagccgcaag gacatatcta attttcatgg ttttcccttc
      121 ttttgatagg tttgaagtaa tggtttgaaa gtgacagcgt ccagcttgcc ttccgcgaac
      181 atcgcccgca gcaaacgctc gtaatcgtcc tcgatttttt gattgtccag gaccgtgatt
      241 ttcgcgatca accggtccag gcgcaatcgc aaggtcggat aagtcacgtt gtaagccgcc
      301 gccaattcct taagcgaccc ggaagcaagg atgaaccgct tgatgaacgc gaggtcttcc
      361 tcttccaaga aatcaatcca acattggctt ggaggtttgt ccatatttaa caaaatcaaa
      421 ctatgaagct ataatattaa gaataatatt aaagtaaact ttaattttat taaaagtgaa
      481 aaaacaaatt ttatggtgct ttctgtccca tgttttagga caggtttagg aaggtgcctc
      541 ttaaacactg ttctcccggg ggggagacgg tttgggtgag ggcgagcgtc atcccaaaag
      601 catcggcatc cgaattgctg acagttctgg gcttgttccg cctgtttgga tttgattacc
      661 atgcggcgga taagcgctgg aaaacgccgt gatggcatga gaaaatttgc attaaaagct
      721 tttggtgtcg gcgatggctg gccttgtgcc gaccgcaatc atgcctcatt tttataccgt
      781 ttgggagacg tctccatcct ggtggattgc ggcgattgca tcagccgcag ctacaaggcg
      841 agcgggctgg attacaacct cattgaccgc atcttcatct cccatctgca ttcggaccat
      901 gtcgccgggt ttttcatgct gatgcagggt ttctggctcg aacagcggaa aaaagacctg
      961 cccgtgagca tgcccgccga cggcatcaag cccatttcgg aattgctgaa tgcgggattg
     1021 attttcaagg aactgttgca attccggctg cggttcgagg cgttgagggt cggcaaaccg
     1081 gtgatgacca aggacgttcg cgtgacgccc tacccttcaa cccacctgga acaattgcgc
     1141 cgctcatttc aaaaacggta tccgcaggat tatgctgcgt attgttttct gatcgaaaga
     1201 ggcaagctgc gcatcggtca tagcgcggac atcgggtgtc ccgaagattt ggagccattg
     1261 ctgaaaaaac cgctcgacct gctcgtttgc gaactggcgc atttccatcc ggaagatttg
     1321 ttcctctatt tacagggccg cgacatcaaa aaaatccttt tcatgcacgt cggacggtcg
     1381 tattgggaag acttgaagaa aacacaacgg ctggcggcga aaatgatgcc ggacatccca
     1441 ttcggctttc ttcacgatgg ccaggaagtg ccgctttgag gcttctggct ggactcctca
     1501 aagcggggct cttcaatcat tttaaaaaaa tatttttgtg ccttgaagcc accaagtttt
     1561 tggaaaatca cgaattagcc cgtaaacatt ggtggaaccg gggcttcgga ctcacttttt
     1621 tgcctgcgaa aaaaaatgcg ctgataccta ccgtaggttt tatttttgaa cggttgccat
     1681 ggtaaagtct cttttatgca gcatgatgct gaacatcaag ctggaggttg gaaacaatga
     1741 ttccgccacc gatgaaaaat gaactgaatg tcggagggga agcgaatgca accattctag
     1801 gtttggcagc ccgcatctgc cttggtcaca gcgaaccgaa acgtggtcat ttcggcattg
     1861 cgtcccacaa tggcatcatg cgtaagtact tgaaatcccg cttagaatca cttagacccg
     1921 gtttaacccg cctaaaatgc caggggggag ggtcccgaac atcgaacgtc caacatccaa
     1981 catcgaacgc tgaatgtggc cgaagcgccg gtttgaacat tcgatgttcg gcgttcagcg
     2041 ttggttgttc gatgttttcc gaattggcca gccgtgtcgc ttcgtgtggc ttggcggcct
     2101 tgggcctttt caccaatact ccgccactcc aacactccat tcagtgcccc cgagtcaagc
     2161 cttggtcaaa ccatgcaaaa aatgaacaac ctgttctcca tcaaagcatt actccgtttt
     2221 tgctcgaaat cgagtcaagc ctggtcaagc cattattatg caatcaaatc aaccccatct
     2281 caaccttctc ccctttcgcg ggcgcatccg catggcgagg ctgggctatg atctgctttg
     2341 ttcacggacg aacggagcgc ggctcgatga gccgcagcaa cgtgcgacta acaggacacg
     2401 ccaaactaat tcatgcgcac ctcgtccttc ccacattgct gcggctcagc gacccgcgct
     2461 ccggggcagt tcgcgaatgc gccctttcgc cttaaaaacc gcctgcgtcc ttgcaccgtg
     2521 aacgttactc cataaacttc ccgcgatgtc gcaaatgcta aaatcatccg gcgcgttggc
     2581 cgccgcgacg atgaccagcc gggtgctcgg catggtacgg gaaatcgtct attacgcctt
     2641 catgggtgtt gggatggtca acggcgcgtt caccctggct ttcatgattc ccaacttgtt
     2701 ccggcggctg ttgggggaag gcgcgttgac ggcctcgttc attcccatct tcaaggagaa
     2761 ggaacgcacg agcaccgaag ccgagatgtg gcgcgccgcc aatgccgtca tctcaggcat
     2821 gatggcggta gccagcgtga tcaccgttct tgcaattctt ggcatcagcg tggcgcttgc
     2881 aaccaccgag tttccggaaa aaaccgaact catgctgcgg ctgcttcgca tcatgttccc
     2941 gtacatgctg ttcatctgcc tgacggcgat cttcatgggg atgctcaatg cgcgcggcca
     3001 tttcttcatt ccggcaatgg gcgcggtggt gatgaatgtg gtcatgatca gcgcggttta
     3061 ctttctggcg ccgaggatgg gcgcaacact ggaacaaaag attttcgggc tggccatcgg
     3121 tgtgctggtg gcgggagttg cgcaagccat cgttcaacta ccttcgttgc gcggagaagg
     3181 cttccgttac tattgggttt caccgtggaa tgacccgact gtccggcgtg tcatgcgcca
     3241 gatgattccc ggcaccatcg gcatcgccgc gttccagatc aatgtgctga caacccagag
     3301 tgtcgctttt tgggtggacc cctcgatcgt cgcttcgttt caaggcgcgg tccggttgat
     3361 ggaattgccg caaggcgtgt tcggcatttc cctcgcgacc tatctcctgc ccaccctcgc
     3421 cggcctggct gcggataaaa aatatgacga ataccgcaag acacttaatc aaggcatcga
     3481 tcacctggtt ttcatcaatc tgcttgcgac gattctgctt ttttttctcg ctgaaccgat
     3541 catccgcctt ttgttcgaac acggcaaatt cattgcggaa gacactccca aagtcacttg
     3601 ggcgcttgtc tgtctctcac cgggactgct cgccttttca ctcgtgaaca ttttggcgcg
     3661 cgcgttctac gcgttgggcg acaccaagac accgatgaaa atcagcattt tctgcctggt
     3721 catcaacctg atttttgcgc tcgtgttgat caggaaattt cagcagggcg gccttggcat
     3781 ggccaacagc ctgagcgcct gtttcaacgt gtcgctgctg ttctatgcgt tgcgaaaaaa
     3841 attgggcaaa cttgaattcg gcgcgttgcg gcaaatgctc tggcccattt tcgtggcggg
     3901 aatttttgcc gcgctgaccg catgggccac atggcatcaa tgggacaaga ctttcggcca
     3961 ctttggattg gcaagacgat tgggcgcggt gttcattccg atgacgctgg catcgcttgt
     4021 ttattggctc gtggcgcttt tgttcagagt gccgcccgcg accgagttgg gcggactcgt
     4081 cctacaacaa ctccctttat tgtcgaaagg caagtcgctg aaaacaattg agaaataatt
     4141 ttaaaagcgg ctgaaaacgc aaattttcag ccttgctgat gctctttgaa aagttggatt
     4201 tgatttatgg cgcagaaccg gtaaatggtt gggaagctgc gcccccgtag ctcaaatgga
     4261 tagagcagcg gtttcctaaa taagttcaag ggtttttcgc gagatttttg cccgctttag
     4321 ctatcgttgg ttttcattga ttttattgac gataattcgc ttttcggttg aagaatcctg
     4381 gcagtcgact tcggctccca tttgctactt tttgccataa aaaaggaaaa aggtatcaga
     4441 tgcagtatca gattcgttcc ggcactcgga gcggaaaagg catggtgccc ccgcccggaa
     4501 ttgaaccggg atctaccgct taggaggcgg tagctctatc catttgagct acgggagcaa
     4561 gagcgcaccg gaagcagcat attgattcct tgatgtcttg caagctaacc acgcccgaaa
     4621 gtttaccggg aaccttccct tcaaaacgag gatttaaatg taacgagcgc tttcgatctt
     4681 gaatctccga tcttacagga cacccaaaca ctctgacacg ggcttccagt cggccgcggt
     4741 ttcgtcgaga tacattttga gagttggcaa tgcatttgcc caacaattgg aaaaaggcgg
     4801 ttgcaggacg tctttcaatt cggcaggctc gggcaggcgg cgttccactg ccagtgcgca
     4861 cgctatcccg agcatggcgt caagcaaagc tggccgcgcc tcgtcaccga atccaccttc
     4921 gccaaggacg cgggccatct tgagtttgcg ggtggcgcgt tcgcgaagca atttcgccct
     4981 ggcaagttct tcggtggaca aggaggcgga ctcggtgatg tctgaaactg gaaacagcgg
     5041 acgggtggcg cgggtggtct tgttgatcag acccgcagca atcaggcgct gaatggcttc
     5101 atctgtggcg cggtcgatta cttccaattt tacaggagcg agcgggtcgg ttttgccagg
     5161 tccgaacaaa tccgcgtgca aggagttcag tgtttcgcgc cattgtgcgg gttcgcgttc
     5221 cacaacgacg accaacattg tatgggagcc ggtttgtgga taacgttctt cacagcggat
     5281 gagggagcca ttgatgcgtt cggaggcgag ttgagcgaac gccacggcgg gatctgccgg
     5341 taatttcttt tggttgatgg gtgaaggcgt cctcgatggt ggatggccgg aagtcaattt
     5401 tgcgggagtg accagttgtt gcaaccgctc gacaaatgcc tggcggccgc ttcgcagctt
     5461 gatttccttc acttcgccag gcttgtcgag cacgctcatg gccacggact gtttgagggc
     5521 gagcgtttcg agcatgcgat gctcgatggt attttccgaa atcagattga atactgtgac
     5581 cggccgggtt tggtgtttgc gccaggcacg ggcgatgcgc tgctccaact tggcgggatt
     5641 ccacggcagg tcacaattga tgacgacgct ggcattctga aggttgaggc cggtcgcccc
     5701 gcaatcggtg ctgaggaaga cgcgacaaag cggatcgtcc ttgaatgaat tgatttccgc
     5761 gcggcggcgt tgttgcggta cactgccagt gtgccaggcg tggccgaggt cgcgctgctc
     5821 gcaaagatca cgtacgagtt cgagcatgcg ctcccattcg gagaaaatga tgaccttggc
     5881 gtcgttttcg ccgcactcct caagcagctt tcccaattca cccagcttcg ggcaggtgcg
     5941 gtcttcggga tcgagaatgt agttggtgtc gcaaatcatg cgcatcatgg ccagttcgcg
     6001 ctgaagtttc tcctgttcct gcttggtgag cggacggcgc agcgcgaggt tgacaagttt
     6061 catcacctgc tgctcgtgga cggcatagtt ttgtttctgc aaatcatgga gcggaacaaa
     6121 gtggttgtgg tcggtgcgct cgggcaattc cgtttccacc tccaccttgc gtcggcgcag
     6181 catgaagggc ttgatgcgct cgtgcagcaa gtccaggttg cggcagccga tgggacgccc
     6241 gcgttcatcg agttcgtaaa aattccgatt gaagcggaag agcgggccga gcacggcagg
     6301 attgagaaaa tccatcagcg aatgcaactc atcaatgcgg ttctcgatgg gtgtgccagt
     6361 aagaatgaat gcgtaacggc tgcggaggcg tttcaccgct tgggcggttt tggtgctcca
     6421 gtttttgatg cgctgggctt cgtcgagcac tacaatgtcc ggcgcgagtc gtgcgttcac
     6481 atccagcgtg tcggccacca tctgttcgta attgacgatg gtgaaaaagg gcgtgaccgg
     6541 ggtctgatcg taagctttga ggcggcgaat cttcccgcca aaaaccatct ggtaatccag
     6601 ggtcgtgaac ttgcgaatct gttcctccca ttccgttttg agtgaagcgg gagtgacgac
     6661 caatacgcgg cgcgcctggc ccagacgatg caagagcgcg caagcggcga ttgcctggat
     6721 ggttttgccg aggcccattt catcagccag caacgcgcgt tccgtgaaag ccagatgcaa
     6781 catcccttcg cgctgatagg gaaacagtgg cacggtggtt tcgtgcgccg gccattcgcc
     6841 gctttgcact ttgagttcat actcgtgccg aagttggcgg cgctcgccga cgcggcggtg
     6901 gttttccaac cacggcccga cttcctgtga aatgcggatt tgcggatgat cactttcccg
     6961 cagttggcgt aacgcgtcaa ctgcggtttc gggtgagtca ttcagcaagc ggccatcggc
     7021 gtcaaaccat ttgctgaccg cgtaaggcag tttgctgcag ccatttggca cgcatagaat
     7081 atttgccgcc gcgtcaactg ccacctcaat gcggttcgat ccaaacgtct gcgcggattt
     7141 aaagtgtttt ttatggcgtg cctgcaaata aagcaggacg gcctctgtat gtttgcagag
     7201 cgcaagtcca ttggtccgga aatccacgca gtcacaggcg cactgcgggc cgttcaagcc
     7261 gcgaatctcc accgagtagg tcagaccgct ggcggacttg acgcggaaat tggagaaaat
     7321 gggatgccgc gcgtctgtat tggtgatgac gaaggattct tcgcgcgcac gctggcggcg
     7381 cttgttgatt tcgtcggcat cggtcgtgcg ccaatcatgg acgggcggca atttaattct
     7441 taaatctgct ttgggcatct tgcgcatgtg gacagcatga caaggaattc aatgcggtca
     7501 atggagacgt ggcgctgctt tacgtgaagg tgggtgaaaa tccggacaaa cgaccggggt
     7561 cacggggcga aactgaaaat actgccagga tgctcgacgc gtcaacggcg ttgtggtaaa
     7621 caaggtgatg cctttcagct atgctgagct tgagcagttt tatgagaagt tagtcacccg
     7681 cgcccgcagt cgcggcatcg cctgcgccat caccagcggc atggcctgtg tggcatttgg
     7741 cgtgtctgag gccacgaaag actgcgactt gctttgcact ccagactcgg ccgatgatct
     7801 cttgggtctg ttaggcgaga cgaccttggg cggacatttg cccagctacc gtggaaatct
     7861 tactccgcca ttggatgccc gctggtttcg cggtggatgg acgagccact tcatgtggcc
     7921 acctgcggaa tccggggcgt atttggacat ctttggtgtg gccccccgtg gcagttctcc
     7981 gtgggaggca gaaattcagg gattttacgc aagtccgcac acggtggccg aaatgaagcg
     8041 caccaaccgc gataaggatt ggccgtttgt gacggcgctc ggcgcccgaa tgatcgaaat
     8101 gggggatgag cgtggttggt tgcatcttta cgatgagaag ctgttacgcg cgttctcaga
     8161 aacaaaacgc cccgcagctt taatcaaaag gcgtcccgtt ttggaactgg cggcaaataa
     8221 tgatccaaag ctgcgacccg cactgcgcgc cgagattgag tattggcacg aactcgaccg
     8281 cgttcgcatg aatatttatg aaaatgcggt gcggcgatat atggtcgaag tgcgaaaatc
     8341 ccatcttccg ccgagcgctg gcttgccagt tcaacatgat ctccgcgtcc gatgcgccga
     8401 aaagtacctt ccggaaaatc caatgcgtga tttcggggtt acacgcatga tttcagaagc
     8461 ccgagaggcg ctttctcaaa tcatcaatcc cgccgcaatg gagtggttgc cagatgtcca
     8521 cgaacacttt acattattga cggcatgaag aataccgggc ggcagaagaa aaagaataca
     8581 ccaattatgg ggcgaagatg ggcggtggcg atctatgatt ttcacgtccg cgcctttgga
     8641 gaggagatgg ctcgtgtgaa tcgtttgccg cttgctgccc gccgccggta cataggcgga
     8701 atggttgacc acgccattca aaagggcgtc aagtctacca aacccgcttt gggcgtcagt
     8761 ccatagatat tcggcacagc ttgctatttt cacgatggac gagcgtcgca agaattgtgt
     8821 caagaatatg gtccaaatgt ttgcccgtgt cttcggccag gaagcgcaat acgaaatatc
     8881 cattccgttg cagcagggca tctttccgcc ggtcccgccg ataagcctcg gcgtcagcaa
     8941 aatgctgagc gccatccaat tcgatgacca cacccgcctc cgcgcaaagg aagtccactt
     9001 ccatcttccc gctgccatca aatgaaatcg gcaacttctc attcaggcga aagcggcctg
     9061 ctgtttccgc caatgtttct agtcgacgaa ataaaaatgc ttcgctggcg cttcgggcgc
     9121 gatcttctcc ctggacatcg accgcaggag acttggccac atgcacgaac agatgtgcca
     9181 gaggaacgtc cacaccgtcg cgaatgagcc ggcgaacact ggccgcgtaa tccttcttcc
     9241 attctggatc gacagggagc gggatttctg caggccagcc aggcagagcg ctcccgggca
     9301 gcaggatggt gtatccgagc gattcatatc cgcgacagcg tttatcgaac atcctggaca
     9361 gcatggaaac attcagatcc gcatagtcat agacacgcac ttcgcgctta ctctcgtgga
     9421 ggcggtgaag tcgccccaca tactgagcaa ttgttccacg ccacgaaaca ggcagagcta
     9481 ggaacaatgt gtccaatcgc gaatcatcga atccttcacc aatgtatttt cctgtcgcga
     9541 gaatcacacg cggcgaagtt gctggaagtt ccgccaagag cgtgcgcgcg ttgttcaatt
     9601 cctttctgct ttggcctccc tgcaaaagaa tcaagtgttg aatatccggc gacaacactt
     9661 gagctaactg ctgtagatgt tcaattcgtt cggtaaggac caaaggtgaa cgtccgtcgt
     9721 tcacggcgct cacgacgtcg gcgcagatca tttggttgcg cttctcgtca ttagtcaaag
     9781 cctcatacaa ttgatgaaat tccgcacgcg gatcgggatc cgacgccgca ctggcgcgaa
     9841 aagccgtcgg acgcaccagg acatggtgaa aaggtcgcgc cgccgcctgg gtcttcgcgt
     9901 tcacccgatg ccgaaccggg ccgcactgca tgaaaatgat cgggtggtgg ccgtctttac
     9961 gaccaaccgt cgcggacaat cctgtgacaa actttgcttt tgcccggcgg gcaaccattt
    10021 caaaactgcg cgccgaaaga tgatggcact catccacgat cagatggccg tagtctccca
    10081 tgcgatcatc cacgactcct ttgtgaatga ggctttgcat gagtgcaacg tccaatgtgc
    10141 cggtcagctt tttgcgtccg cctccgagtc gcccgatggt tttggcgggt aaattcagaa
    10201 atgtggacaa gcgttcgatc cactgttcaa ggagttgctg gcggtgaact aaaatcagcg
    10261 tgttgacgcc gcgttgagca atgagccaag cggccaccac agttttacca aacgccgttg
    10321 ttgcagcgag aacgcctgta tcatgccgca acagtgcctc gacggccact tgctgatcgg
    10381 gccgtaatat accgcaaaaa gaaacgccta gagaaacgcc accgaatcgt tcatcacgaa
    10441 gaaccggctg aatcctgagc gcctcaagcg ttttttgcac gtcgtccaga caaccgcgcg
    10501 gaacggcaaa atgctttggg tgatcctcgg cgcaggcgat gattcttggc ttgtcgtaag
    10561 tcgaaaggcg catagcttgg gcgcgataga attcggggtt ttgaaacgcg gccaaacgga
    10621 gcaaccggtt gcgaagccca ggcacaaggc cttctttgcc gatgtagatt tggtctccaa
    10681 gtattaattc aagcttttcc ggcagcgacc cggaaatggg cggttccttt tgacgacgcg
    10741 atggtggtgc agtccaagga tcgtcatcgt cctcttccgg cgcggcaacg cgcacgccta
    10801 cgattcggcc tttggattct gcgttgcgca caagaacctc aacttgcgtc cggctcattc
    10861 gactcaccga agccaggaag gaccattgat cggtgtgagg gttgaacttt tcattgagga
    10921 aaacagtgtt accacgctgc ctcgcctgtt tctggagggg cagtgcgatt aagtttccaa
    10981 acccgcccct gggcagcgta tcttggttgg gaaataaccg gtcgtacgaa ttgagaccaa
    11041 tttccggacg gtcttccatc gtctcagtga ggacatgcga acccagtttg cgcgccagta
    11101 tggcgggaat ggcttcttca aagaacaacc agatatgacc gccgttgcca gatcgcgacc
    11161 gttccaaggc ggcggggaca tcgatctggc ggcaggtttc gagaaaagct gccgcatccc
    11221 gctgccagtc ttcatcgtcg aaatccacgg ccaaaaagaa acaagtctcg tcctgtaaca
    11281 tggcataagc acccatgaca aaatcgaaac cattgctatc acgccccgaa agatgccagc
    11341 cgaccacctc gtcagtgatc tgcaaaaagc gccgattggg gcaattcgtg catttgattc
    11401 gcggcttttc acaaacacca cgaacccatt cattggcgca ggctggcgca taaccggctt
    11461 tcccagtttt gcggctttca aaccggcgtg gatacacatc ttcccggccc cgaaaaagag
    11521 atcgaaacaa ctcaattttg gcagcgggcg aagaattgtt cgtgacggtg gggattgacg
    11581 gatttaacga aaccgcgttc ggcatcaaag agcacgcccg gttgccgctg ttttggtcaa
    11641 tcgtttccac acggcaacta taatataaat gcgattacgc tggcgaattt gttttcgtga
    11701 taaaagcggt ataaaagccg ccagtctccc aattcagaaa aaacggtatc agatgaagta
    11761 tcacatcagc ttggttttgc ccgcttttgc tattatttgc tttagtttcc taaaccgttg
    11821 atcccagttc aattctgggc gggggtacca agcttctagt ttacggcagg cttgctcgat
    11881 aaaaacgctc aggcaaattg gcggcgttgg tatccacaaa ttgccacgag ccatccgctc
    11941 cggccaaatt ggtggagacg gattgccaga ttgccggact caaagagctg gttgccagga
    12001 tccgatgcga catgccgggc atcccgccgc attgcaggtt gatgctgcca ttcggatttc
    12061 gaacaatttt cgaaatcatg ggcgtgggca atgtgaccac caaatcgtcc atcgcgaaac
    12121 gacctctcgc gaccaggtcg ccaaaataat tctcgttcgt gtccgccgcg gcataccagg
    12181 tttgcggatt tgtggatgga acgccattca cccaaacgcg gtagtagaat attccattcg
    12241 catcacgatt gtaattcgct tcaactgttg cgcggaaaaa ttggtttgaa gcgatgctag
    12301 tgtcagcaag ctccgtccag ccgtttccaa gtgccggagg attggtgcga ttccatacag
    12361 ccaaatgacc gttcgtggtg atgcacaacg caaatggtgc gttgctgaac gtcagcatca
    12421 gcgggtcggt ccagtattta gcttggagaa tcatatcgac ccagacgttc gtgtcaggcg
    12481 attgacggaa gcggtttgtg atcgcgccat ctacttgcaa catctgttgg tgcgctcccg
    12541 gtattggaaa gatgtttgtg taggtgtcgg cgtttgtgga gatgatgccc atcgcgctgt
    12601 cctcgccaaa ccaaccgttc gtgccgacaa ggctgtaccc gttggtgtaa aattcaaaag
    12661 attcgcgata ggaaatcgcg ttgttcaagt tcgcgatgtc ttcggtcaac gtcagcacca
    12721 tttcatcccg gtccaaggca agtttcacga tttggtcggt gtaaggttgc acttgtccgt
    12781 cggcgtcgtc cctgccgatg taaacgaaat cgcgcaccgg atcataagcg atgctgcgcg
    12841 cgaaaacttc gccccaattg gtggagtttg ttggattgta tccccatgca ggaacgttcg
    12901 tataagttga cggcaaaagc ggaaggctgc cggtttcaac gggcgcgttc gtccccggtg
    12961 gcaaacggaa acgatatacg tacgccggat aggaaaggtc gctggaaaaa taaagcagac
    13021 cgcgatcggg cttgatgact gcgacgtggg catagccctc attggtgtgc atgtccgtgt
    13081 aagaaaccag agtgggagag ttcgtgccgt tcaacgccca cttgtacacg cgcccgtggc
    13141 cgaacaggag atcggtgccg tcgctgtcgc aatatccgta accattcgcg atatccagca
    13201 cgagggcgcc aagggaccgg ttggtcgagc tgtccagcga aacgccgccg taattgattg
    13261 gagcattcga acctgcgccg agcgaaaact ttgccacata aggaggcagt gtggtcggaa
    13321 cgaacgtccc gtaataagcg catcggttgg tcaggtcaat cacaccatat cccaaggggg
    13381 gttgttgagc cgttgtgact gccgagccga ttatcgcgct ggtgttcgga aattgattga
    13441 gcgcgagctt ataaagcgtg gacgaggcgt tgccggtttc cgtcattgca taaagataat
    13501 gattggcggg gtccgtggta tcgatgagaa gttgcgtcaa aaaagttgaa gagccaaacg
    13561 gcgaactcat gattgctccc aattgaggcg cattggtccc gttcgccaaa atctggtaaa
    13621 tgctggttcc gacggcataa taggagcagc cggctgaaga atccatgacc gctgaaaaag
    13681 ccagccttcc aagactcacc ccggctccga cctgcgtcgg caacggcgtg gtgatgttca
    13741 ccttgtaaac atattttgcc gcaaaataag caaagccatt cgtaggatca attgcggctg
    13801 aataaaaacc tttgttgtcg tcaaccgtct ggaccaatgt caacgcgcct tcccgccgca
    13861 tgttggcttg cgcaatgatc gacggcgcaa gcagcatgcc aaaacaaacc gccaaaaaac
    13921 aatgaaacaa tcgggttttc ataatgaagt cgcaaatctc caaattgcga caaaatcacc
    13981 tgcgaggcaa aaagcagcta acatgccaaa aagaatgaga atggcggcaa gataagcgcg
    14041 cgctcaacgc ggtggccaag tgttcaacac ctgcgcggca tcgacccgct gttgctcacg
    14101 cttttcttcc ggacgccatg aatatcatgc aggcggccac ttcccggcgc agacgttccc
    14161 gatggtccat gtccaggccc tttggatcga aacgatatcg gtaatgcaag cgcaggattt
    14221 cctccaacgg ctcattcaag cgggacaact ccggggctgc ggacgtgcgt cgcagccagt
    14281 cggatagcgg ttcgccggga cgccgcgccg cgccgcgctg gaccagcgcg cgttccaatt
    14341 catagaattc ggaatccatc ccaggccggt gcatgggtat ggatttttgt tttgattttt
    14401 tgcgcgcgcg ccgggcattg ctgcgaaaaa ggatttgaaa gagcatcagg gccagcacgg
    14461 gcacgaggac ccatggcaaa agctgccgga gacgactttg gccccagcgg aatttggaaa
    14521 actcgaacac tgcccgggac caccaatcgg agatgaactg gaatgaagag cgctgttcgc
    14581 gttctgcttg catccagatg ccaggagtgg tgtcgaagtt ctcccacctg ccacgccttt
    14641 cattatatac gcggcaccag gcatgggcgt cattttgccg gacgacataa ccgctccccg
    14701 acttctcatg caccgcgtag cccacagtat aacgcgcggg gatgtgaagt tgccgcaata
    14761 gaagcaccgt ggccgtggcg aaatattcgc agtgtccctt gtgcgtttgc aacaggaaac
    14821 gtccaagcgg tgtcatgttg gtgtccatca cccttgttgg ctcctgccag aggctgtaag
    14881 tgaattgcgc gccgaaaaaa tggctgatgg ccagcaactt tttgtcgtca tccagcccat
    14941 ccaaatgcaa ttcggaaacg acatgctcca cggcggaccg ttctttttca gggatggaca
    15001 aatcttccga gttttcggag ttatccggcg ggtaatccat tgcactgccg tatccgtaaa
    15061 gcgcgtcgaa aatgaccaat cccggacctt gggcatggac ggttccaaca tggtttttct
    15121 gcacaaaaat cgcctgcaat tgttcgagtc gcgcggtttc ggtcggcaat ggcagcaatc
    15181 cctttccgcc cgcaagataa caagcgatgt tcagagtgcg caggggattt ttggtttcaa
    15241 acaaaggata attgccgctg ttgcgcggct gatcattcac atcggtgaaa tcatcttttg
    15301 aactgccgga gtaccacacg ccggatttat attgccggta cgccgcttct cgcaaataag
    15361 ccggcggcat ctcgtcattc atggttttca gtcggagaac aatcttatgg gaggttttga
    15421 tgcggccaat ctgcccgatt tcagtccggc tttcgatagg gtcgaagccc tggtccacga
    15481 ggtcggcaag ccattgcgga ttgtattgga caatggcgcg ttgcagttgc accaaaccgc
    15541 gttgtccgcc aaatcccagc gacatcgcca gcgccatcgc gcccgcccac acaatcgtgc
    15601 tgaatcgccg cgaccgaaac ggccacaatc cccacatcaa taatccgcac aaaccgaaga
    15661 aaaaattttc gccgctggaa ggttgaggat gggtgcatgc cgcgaacagg cacaagataa
    15721 agtatggata tgccatgttc atggagcgca tcggcggcat cggctgcgtc aatttcttca
    15781 attttttcca gcgacggcgc aggattgagg atacgacaat catcggaatg ccttcctggt
    15841 tgctgaacgc ctgcgccgcg atgaagaaga aataaaccag cggcagccag cggatgacca
    15901 agaccgcagt cctggtgctg acggtggcga tcgtgttttg cgccatgaag ccggcgctgg
    15961 aaaatatgcc gcgaagattc gccggcccgt tgctcgcgct gaacgcgtaa gcgaggcagg
    16021 ccaccagaag cagcgtgcag aatgtccaga tgcggctgaa atcttcggtg gtcatctccc
    16081 aacgcagcct gacataacgg gaaccttcca gcactaccgc aaggaccgtt gcgacgataa
    16141 aaaattccgt ctgccagccc caaaaaagca gcgctgctcc aaccagcatt ggcggagttt
    16201 tcataattgc cccagtcctt cctcgatcct gccaagaacc agcgtgtgaa agtgcgcagg
    16261 gtcgtcagcc atcggtcccg gctcgagcgg cttgtcgggc aatgattcga cgatgacaac
    16321 caccaccacc ggcacacccg aaaacctgag ctttttgacg aagttacggc gcgattcgtc
    16381 ccattctag
//
LOCUS       DASZMM010000017         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2480570, whole genome shotgun sequence.
ACCESSION   DASZMM010000017 DASZMM010000000
VERSION     DASZMM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480570"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VG754_00390"
     CDS             complement(<1..1214)
                     /locus_tag="VG754_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWF17689.1"
                     /translation="MAGKRKRGGASLEMSLDQDWLFGGKFKEGSDQPRFDDAAFSHVT
                     LPHAVAKLSWENWKPESWQDVWIYRRHFQLPENFRNRRVFVDFERVMTTATPVINGYA
                     LDEHRGGYLPFSNELTTLLKDGDNVLALKVDARWQNVPPDGNPKGTAHVDYLQPGGIP
                     GSVTLRAVPQIFISDVFAKPVNVLELYRSVEVTCLIDAALMPEKPLSVRVDMLDGHKR
                     IATVSEPLATNKAGVSEIKLTLSNLGNVKLWDVDAPKLYRVRTTLLMDGHPLHEHHTR
                     IGLREAKFTADGFFLNGKRLRLFGLDRHEIYPYAGLAMPGRVMRHDAEILRREFNCNI
                     VRCSHYPQTEAFLDACDELGLMVWEETPGWGYLGDDAWKEQVVQNVHDMILRDRNHPS
                     IIIWGVRVNESP"
     gene            1623..2837
                     /locus_tag="VG754_00395"
     CDS             1623..2837
                     /locus_tag="VG754_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HWF17690.1"
                     /translation="MSQSKNDYSWAEFLDYMEGNDLDVLSLPRSKPAKVVAREQPIWG
                     VLVAVVVTLAAIWLGDLNFWPFTVHTATGKTSHPVESVMIAIILGMVIGNLWSLPKLL
                     QPGIKFSVKKLLPLAIVLLGVRLNFADMRKLGLAGLALSFLETVVALGLLFVLSRWMK
                     LPRKLGTLLGVGTAICGGTAIVAAAPVIEAEEKDVAISVATVTLLGLLGMFFLPLIGH
                     ALDLTSKQFGMWAGLAIHQTPQVIAAGYSYSPSNAEYSPVAGDTATIAKLARVCLLAP
                     VVFILGFLHARSKTQGQSRTGRKINYLKLFPMFVLGFIGMALLKTLGLIPDITVHDSA
                     FLGAGAHSYYLGNAAEQVSKFCICVSMAGVGLETKFSAMRQTGLKPFVASLIAVLVVA
                     ILVLALIKVLGI"
     gene            2980..4194
                     /locus_tag="VG754_00400"
     CDS             2980..4194
                     /locus_tag="VG754_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWF17691.1"
                     /translation="MKLTLEQMASLESEILKRVRARPGFSRVALARQLQIAPSTVGNY
                     VGRLISEGFLSESPAVPENGEVEAGRPATALRLNPDGGHFVGVDFEARNIMAMSVDFS
                     DTPIKHVHDQIEETDTIAQILRKIETAIASVLPDHQSKLLAIGIGVPGLVDPINGVAI
                     EYKYIHNWRNVQLTEPMAEKFGVPVYLENTIRSMALAEMWFGQGRGERDFLCVGIRSG
                     IGAGIIAGGQLQCGSAHRAGEIGRWRFPWPKTSMTRFFTNNGTANAEVELQEVGSVRA
                     ILVALERAREAMMKTMLSAHPLPLKFADVVRAAQQRDALTTQILAVAAETLGQAVSQL
                     VLTLNPSKVILAGPLTLLGDTLLHPLRHRAEELLQASGADLPAIVNSTMGEYSGALGA
                     AALGVHEWKPTR"
     gene            4304..>4528
                     /locus_tag="VG754_00405"
     CDS             4304..>4528
                     /locus_tag="VG754_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HWF17692.1"
                     /translation="MKIFLDTADVKQIKEAYGWGIIDGVTTNPTHVSKTGRPPAEVYK
                     EICSIVNGPISLETIGLTADEILKEGHQLAS"
BASE COUNT         1015 a         1237 c         1216 g         1060 t
ORIGIN      
        1 tttggcgatt cattgacgcg cacgccccaa atgattatcg agggatggtt ccgatctcgc
       61 aaaatcatgt cgtgaacatt ctgcacgacc tgttccttcc acgcatcgtc gccgagatac
      121 ccccaaccgg gcgtttcctc ccaaaccatc aggcccaact cgtcgcatgc gtccaaaaaa
      181 gcctccgttt gcgggtaatg cgagcagcgg acgatgttgc aattgaattc acggcgaagg
      241 atttccgcgt cgtggcgcat caccctcccc ggcatcgcca agccggcata cggataaatc
      301 tcgtgccggt ccaaaccgaa caaacgcagg cgttttccat tcagaaaaaa accgtccgcg
      361 gtgaacttcg cctcgcgcag accgatgcgc gtgtgatgct cgtgcaaagg atgaccgtcc
      421 atcagcagcg tggtgcgaac tcgataaagc tttggcgcgt ccacatccca aagcttcacg
      481 ttgcccaagt ttgacaaggt gagcttgatt tcgctgacgc cagccttgtt agttgcaagc
      541 ggttcggaaa ctgtggcgat gcgtttgtgc ccgtccagca tgtccacacg cacgctcaac
      601 ggtttttcag gcatcaatgc cgcgtcgatc aagcaggtga cttccacgct gcgataaagt
      661 tccaggacgt tcaccggctt ggcaaaaaca tcgctgataa aaatctgcgg aaccgcgcgc
      721 aaggtgacgc ttccgggaat gcctccgggt tgcaaataat ccacgtgggc cgttcccttg
      781 ggattgccgt cgggcggcac attttgccag cgcgcatcaa ctttgagcgc aagcacatta
      841 tcaccatcct tgagcaacgt ggtgagttcg tttgaaaaag ggagatagcc gccgcgatgc
      901 tcatccagag catagccatt gatgaccggg gttgcggtgg tcatcacccg ttcaaagtcc
      961 acaaacacgc gccgattgcg gaaattttcc ggcaattgaa aatgcctccg gtaaatccat
     1021 acgtcctgcc agctttccgg tttccaattt tcccatgaaa gctttgcgac ggcgtgcggc
     1081 aaggtgacat gcgaaaaagc tgcatcatca aagcgcggtt ggtccgagcc ttccttgaat
     1141 tttcccccga aaagccagtc ctgatccaac gacatttcca aactggctcc tccgcgcttt
     1201 cgcttgcccg ccatgctggc gcatcctccc acagtgcccg atgccagaac aaccgcgcta
     1261 agagcgcaag cgcgaacgaa atctctgcgc gtataaactt gcatttattg gtcagtgaac
     1321 tttagcgaat aatttcgctt tgtgaagtaa tttcttgaat tccgattcag aactgtctgg
     1381 aaaataagaa taagtccttt gtttgtcatc gggttgcttc agaacaaaat caaatttccg
     1441 tggaatggac taaacatcca cagccgttga tttcaggagg ttaagggaca aacaccctta
     1501 ggttctcgtt ccatctacct cactttttca tgattccgtc aaaacgatgt gcgattttat
     1561 ttatttttaa tctggaataa tattgactgg ccacccgaaa ccagttacat ccgttcacac
     1621 gcatgagcca aagcaaaaac gattattcct gggccgaatt cttggattac atggagggca
     1681 atgacttgga cgtgctgagt ttgccgcgtt caaaacctgc gaaggtggtg gcccgggaac
     1741 agcccatttg gggcgtcctg gttgcggtag tcgttacgct ggcggcaatc tggctcggcg
     1801 atctcaattt ctggcctttc acggtgcata cagcgacggg caagacttca caccctgtcg
     1861 aatcagtcat gatcgccatc attttgggaa tggtcatcgg caatttgtgg tcgctgccca
     1921 aattgcttca accgggcatc aagttctcgg tgaaaaaact gctgccgctg gcgattgtgt
     1981 tgctgggagt gaggctgaat tttgccgaca tgcgcaaact ggggttggcc ggactcgcat
     2041 taagttttct ggagacggtg gtggcgctcg gtttgctttt cgttttaagc cggtggatga
     2101 aactgccgcg caaacttggg acgctgctcg gagtgggaac ggcaatctgc ggaggcacgg
     2161 cgatcgtcgc tgcggcgccc gtgatcgaag cggaagagaa ggatgtggcc atcagcgtgg
     2221 cgacggtgac tttgctgggt ttgctgggga tgttttttct gccattgatc ggccatgcgc
     2281 tggatctgac ttcaaaacaa ttcggcatgt gggccggcct ggcgatccat caaacgccgc
     2341 aagtcatcgc ggcaggttat tcctattcgc caagcaatgc ggaatacagt cctgtggcgg
     2401 gtgatacggc gaccatcgcg aaactcgccc gcgtctgcct gctcgcgccg gtggtgttca
     2461 tccttggttt tctccacgcc cggagcaaga cgcagggaca gtctaggacc ggcaggaaaa
     2521 tcaattatct gaaacttttt cccatgttcg tgcttggctt catcggcatg gcgctgctga
     2581 aaacgcttgg ccttattcca gacatcacgg tccatgacag cgcgttcctc ggcgcgggcg
     2641 cgcattcgta ttatcttggc aacgccgccg agcaggtatc caagttttgc atctgcgtca
     2701 gcatggcggg cgtcggattg gaaaccaaat tttccgcgat gcggcagact ggtttgaaac
     2761 cctttgtggc cagcctgata gcagtccttg tggtggcgat tttggtgctc gccttgataa
     2821 aggtccttgg catataatca ggactgtcaa ccacgcgggc atcattttga ctacacattt
     2881 gcaaacattg cgtcgcaatt tgacgccgtt tttgggttta tccgtgattg acagaaagaa
     2941 gcgctcgaca ttattctttt taacaaataa tcagatgcaa tgaaactgac tctcgagcag
     3001 atggcgagtc tcgaatcgga gattttgaag cgcgttcgcg cccgcccggg gttttccagg
     3061 gtcgcgctcg cccgccagtt gcaaatcgct ccctcgacag tcggcaatta tgtcggacgg
     3121 ctcatttccg aagggttcct gagcgaatct cccgccgtgc cggagaatgg cgaagttgaa
     3181 gccggccggc ccgcgaccgc tctgcggttg aacccggacg gcgggcattt tgttggcgtt
     3241 gattttgaag cgcgaaatat catggcgatg tccgtggatt tttccgacac gccaatcaag
     3301 cacgttcatg accagattga ggaaacggac accattgcgc aaattctcag gaagattgaa
     3361 actgccatcg cctccgttct gccggaccat caaagcaagc tgctggccat cggcatcggc
     3421 gtgcccggct tggtggaccc gataaacggc gtggcgattg aatacaaata catccacaat
     3481 tggcgcaacg tgcagctcac cgagccgatg gcggaaaagt tcggtgttcc ggtctatctg
     3541 gaaaacacca tccgttccat ggcgctggcc gaaatgtggt ttggccaggg acgcggtgag
     3601 agagatttcc tttgtgtcgg catccgcagc ggcatcggcg ccgggatcat cgcaggcgga
     3661 cagttgcagt gcgggtcggc gcatcgcgcg ggtgaaattg gacgctggcg cttcccgtgg
     3721 ccaaagacct ccatgacacg tttctttaca aacaatggca ccgcgaatgc cgaagtcgaa
     3781 ctccaggaag tgggctcggt gcgcgcgatt ctcgttgcat tggaacgggc ccgcgaagcc
     3841 atgatgaaaa ccatgttgtc cgcccacccg ttgccgctca aattcgcgga tgttgttcgc
     3901 gcagcgcaac agcgcgatgc cttgaccacg caaattttag ccgtcgcggc ggagacgctt
     3961 ggacaggccg taagtcaatt ggtgctcacg ttgaatccat caaaggtcat ccttgcgggc
     4021 ccgctcacgt tgctagggga cactctgctg catccgttgc gtcatcgcgc cgaagaactg
     4081 ctgcaagctt ccggcgcgga cttgccggca atcgtcaatt ccacaatggg cgaatacagc
     4141 ggcgcgcttg gcgctgcggc cctgggcgtg catgaatgga aaccgacccg atgacgtctt
     4201 tccctccatt ttacatcgcg aaggaagcct gaattccatc attcatttta gaatttttca
     4261 acgatcaacc ctcattcaaa acccgattcc gcagaaataa attatgaaaa tattcctcga
     4321 tacagccgac gttaaacaga tcaaggaggc ctatggctgg ggcatcattg atggcgtgac
     4381 caccaatccc acgcacgtct ccaagacagg ccgtcctccg gctgaagttt acaaggaaat
     4441 ttgttcgatc gtgaacggtc ccatcagcct tgaaaccatc ggtctcaccg ccgatgaaat
     4501 cctcaaggaa ggccatcaac ttgcgagc
//
LOCUS       DASZMM010000018         8215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2757758, whole genome shotgun sequence.
ACCESSION   DASZMM010000018 DASZMM010000000
VERSION     DASZMM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757758"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1159)
                     /locus_tag="VG754_00410"
     CDS             complement(287..1159)
                     /locus_tag="VG754_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17693.1"
                     /translation="MNLRLKAGHGGASSATLDASMDTAEPTWREAWEPLTPRGVAAFA
                     GSSLGRLLLVQLLVALLAAGSVGWFLKTGWFPVVRSAIQAMPPEGDLANRHLAWKGQN
                     PAQLAQNRFLGIGVDMYHSMQLGREAHLQIEFGEQDFRIYSLPGYEVFDYSPDWNYAF
                     NRTALEPWWGAWQPWILVGALVLVVASLMISWTLLATIYCVPVRLFSFFENRDLNLRQ
                     SWLLSGAAMMPGALFLTAGIVGYTFGLMDLIRLGGVFGLHFVVGWIYLIISPFFCPRH
                     PATKKQNGNPFATK"
     gene            complement(1300..1737)
                     /locus_tag="VG754_00415"
     CDS             complement(1300..1737)
                     /locus_tag="VG754_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17694.1"
                     /translation="MQVSRILPDLQCSLLCEEIRQEANGNFFLIGVINYLRVPQVPVT
                     AFKLSLFNRWTAGMGQFTESVRLIAPDQTTVMRKSELKFELKDTALHSTNVTVFGQVE
                     FKTAGTYFIEVLVDDVMKLRYPIPLIVAPQNQNPQNPPAPPPA"
     gene            complement(1779..2702)
                     /locus_tag="VG754_00420"
     CDS             complement(1779..2702)
                     /locus_tag="VG754_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HWF17695.1"
                     /translation="MSADLIDGRAIAGQIQGETARRIEVLKSRGVQPGLAFVRVGEDP
                     ASRVYVGMKEKMCAQLGIFSETRVLPESTTENELVEAVRQLNKDFRIHGILIQAPLPK
                     HIRETEIYSKVLPWKDVDGFSPFNVGKLMLGDTTGFVPCTPAGILELLVRSQTKIEGA
                     EAVILGRGNIVGKPMAAILCQKAKHANATVTICHSQTRDIKAHCRRADILIAAMGVAK
                     FVTADMIKPGAVVIDVGVNRVSDPSAKGGSRLVGDVDFEAVRQVAGKITPNPGGVGPM
                     TIAMLMQNTVRAAEMALEQREKNPPQNKANN"
     gene            complement(2706..3830)
                     /locus_tag="VG754_00425"
     CDS             complement(2706..3830)
                     /locus_tag="VG754_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HWF17696.1"
                     /translation="MKKKFGLILATMVSAALLAQTPPPGGNPSPSTPPPLPAAPDTST
                     QPATPPPSVAPLTPPDAGTTTNAPAKKKTKKKTAKKKTTKEAKAAAPEMPAEPLVPGE
                     PAVAKQNNVNIRGQAHINSEVVGHLQKGDTVTVLEEVTLKHPKTDEPSKWAKIALPGT
                     LHVWVNAPFIDATNQTVTARKLNLRTGPGENYSVIGTLQKGDAVKTAGNTKGDWTQIE
                     APTNAFAFVAAHLLTHKAPEPAAPTNPPPTQVAVGNPPPVAPPSTETPGSVAPPPPNV
                     PLPPIAPPPAPAVEEPPPPRIIEREGLVGGSVSIQAPSHYELKSLDDGTIMDYLYTTS
                     TNLSLARYRGLTVLVTGEEELDERWPNTPVLTIQKIQVVK"
     gene            complement(3837..4544)
                     /locus_tag="VG754_00430"
     CDS             complement(3837..4544)
                     /locus_tag="VG754_00430"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWF17697.1"
                     /translation="MVRLQKFLAEAGIASRRASEKIILEGRVSVNGRTVNELGGKVDP
                     VHDQVAVDGRMVRAKRKLYVALNKPSGYICTRLDERERRTIGSLLPKEWGNLYSVGRL
                     DAESEGLIFLTNDGEFSLRLTHPRYGVRKKYIATVTGRVQPEMLGRITRGVSHEGEML
                     KAEKARLLSANNSNSVVELELAEGKNREVRRLFESQGLEVNRLVRTQIGKIKLGELPG
                     GKWRTLTETEIKSLLSG"
     gene            complement(4628..4783)
                     /locus_tag="VG754_00435"
     CDS             complement(4628..4783)
                     /locus_tag="VG754_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HWF17698.1"
                     /translation="MSQHRSLRAVATMGGKRNVLKRFERVGLLKKRGQWKDGERITGL
                     RKTKPEA"
     gene            5073..5552
                     /locus_tag="VG754_00440"
     CDS             5073..5552
                     /locus_tag="VG754_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF17699.1"
                     /translation="MSKQAKDNFLVLVADDSESDRILLKKAMERATTLRIIGEVANGH
                     DVISYLKGHAHFNDREKYPLPDLLLLDLKMPLKDGFEVLTWLRTQSFQNLTVVVLTDS
                     MHAEHIKRALDLGADLFQVKPKMPHDLNAMILALEEHLQLFARTPTRRTHLPAAMRL"
     gene            complement(5582..6235)
                     /locus_tag="VG754_00445"
     CDS             complement(5582..6235)
                     /locus_tag="VG754_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWF17700.1"
                     /translation="MKNLTLRLFAVAIGCSMLAATNSNAEDFVRYRARPRGAKVHLDG
                     SANIHDWTMDGTIIGGWFEVPANVTIDSSQAAIGGMSGDKLNAKAEANIPVVTLQSGT
                     SGMDQVMQQAMNATDHPQIAFKLSEMTLKQPHSAGTPFQFDTKGDLTVNGVTKPITMP
                     VSIETIDKTKLKISGGPVEINMTDYKVPPPTKFGIFETRPNVKITLQWVVALPKTSK"
     gene            complement(6250..7713)
                     /locus_tag="VG754_00450"
     CDS             complement(6250..7713)
                     /locus_tag="VG754_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF17701.1"
                     /translation="MSENEANQGQNDFNRRDFIKSTASLGSLMLLMGGTPLYADDATP
                     KQAETDFSTEDQPINFAVIGCGTRGKEILQTLALLPGGTRKDGTPNPPNAPVVAICDN
                     YKPAIKRVKDYAPNAQVYDDYKKLLEQKDVECVVVATPSHLHRDIVIAALQAGKHVYC
                     EAPLATTVEDARAIAKAAKAAEKLNFQSGLQMRSDPGKKFVLDFVHTGAVGTPVMARS
                     QWHKRESWRKAGSTSERDKEVNWRLYSETSAGLAGEIGVHQYDLVTWMLGKKPLAVTG
                     FGSILDPKIQDSGDDRDVADTIQTIMEYPRKVNAMYDCTLANSFDGEYDMIYGTGAAI
                     LFRPESLGQNYTPAKAWLFEEVDSQVFGWEVYAAKEQFHKEVGITLSAGASHSTSGKK
                     GIEASPYQESTLHYALKAFAHNSLFTATHAKAFTDAYGADAEGFLDEMAKPENAKGRL
                     PAAGYREGFDATVIAIKANEAIKKREKVMLPKELFDI"
     gene            complement(7795..>8215)
                     /locus_tag="VG754_00455"
     CDS             complement(7795..>8215)
                     /locus_tag="VG754_00455"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWF17702.1"
                     /translation="HSATDTFHGENGVDPYIEMIGGEFVEHTVAKVDCIVVDPKHPST
                     RHFGKVFPVFDEIYMMKNFSPDKVHLLLRLDKVPGSETPGNFPISWCKSYGGGKVFYT
                     ALGHEDAVWENPDFQLHLLGGIKWALGLEPSKIGGAD"
BASE COUNT         1744 a         2277 c         2232 g         1962 t
ORIGIN      
        1 atgaaaggag gatcacgcac aacatgcgcg cgccgcgggt cgtcgcccgc cggtctgcca
       61 ccaagtcgta gaatgaaaag cgcgtgaagc tgccggattt caacgttgcg atcataaagt
      121 ttcttgagca actatcattg acaaaccggc ccgcgggtaa agaaaggact tgcgttttgc
      181 ggtatgattt ttgtgtaagg tttgttccta catacacgcc tgcgcgcaag ttcgaagttt
      241 aaaatttaag gttcaaagtt ggtgacaagg ctggactaac cttaactcat ttagttgcaa
      301 acggattgcc gttttgtttt ttcgtggcgg gatgtcgcgg gcagaagaat ggactgatga
      361 tgaggtaaat ccagccgacg acaaaatgca gcccgaacac tccgcccaaa cggatgaggt
      421 ccatcaggcc aaacgtgtag cccacgatgc cggccgtcag gaacaatgcg ccaggcatca
      481 tcgccgcgcc ggacaagagc cagctctggc gcaagttcaa atcgcggttt tcaaaaaagc
      541 tgaacaaacg cacgggcacg caatagattg tcgccagcaa cgtccacgaa atcatcaacg
      601 acgcaaccac caacaccaac gcgccaacca aaatccacgg ctgccatgcg ccccaccacg
      661 gctcaagcgc ggtgcggttg aaggcgtaat tccaatcggg tgaataatca aacacctcgt
      721 aaccgggcag ggaatagatg cggaaatctt gctcgccgaa ttcgatttgc aggtgcgctt
      781 cgcgtccaag ctgcatgcta tgatacatgt ccaccccgat gccgagaaac cggttttggg
      841 ccagttgcgc cgggttttgg cccttccacg cgagatggcg attggcgagg tctccttcgg
      901 gcggcatcgc ttgaattgcg cttcgcacga ccggaaacca gccggtcttc aaaaaccatc
      961 ccacggaacc tgccgcaagc aatgcaacaa gcagttggac gagcagcaat cgcccgagcg
     1021 aagaacctgc gaatgccgct acgcctcgag gtgtcagcgg ctcccatgct tcgcgccaag
     1081 ttggttcagc ggtgtccatg ctcgcgtcga gagtcgctga cgacgcgcca ccatgtccag
     1141 ccttcagccg gagattcatt aaatctcaga cggattcaga tgaactcaga ttcaagcgga
     1201 atgacaacga agattggagt gttggagcaa tggagtattt ggcagaggga tttccgtcca
     1261 gcacttcaat actccattgt tccactttcg cccggcgtct cacgcgggcg gaggcgcggg
     1321 aggattttgg ggattctggt tttgcggagc gacaatcaat ggaatcgggt aacgcagctt
     1381 catcacgtcg tccaccagca cttcaatgaa ataggtgcct gcagtcttga attccacctg
     1441 gccgaagacg gtgacattcg tggaatgcaa cgcggtgtcc ttaagttcaa atttgagttc
     1501 gctcttgcgc atcactgtgg tctggtccgg cgcgatgagc cgcacgcttt cggtgaactg
     1561 gcccatgccg gcagtccaac gattgaacag gctcagcttg aacgcggtca ccgggacttg
     1621 cggcacgcga agataattga tgacgccgat gagaaaaaaa ttgccgttgg cttcctggcg
     1681 gatttcctcg cacagcagcg aacactggag atcgggtaaa attctggaga cttgcatgtt
     1741 gtgttggatg ggttgtagtg atggagtgat ggggaaaatc agttgttcgc tttgttttga
     1801 ggcggatttt tttcgcgttg ttccaatgcc atttccgccg cgcgaacggt gttttgcatg
     1861 agcatcgcga tggtcattgg cccgacgccg ccggggttgg gcgtgatttt gccggcaacc
     1921 tgccgcacag cctcaaaatc cacatcgccg accagtcgcg aaccgccttt ggcgctcgga
     1981 tcacttacac ggttcacgcc aacatcaatc accaccgcgc ccggttttat catgtcagcc
     2041 gtcacaaatt ttgccacgcc catcgccgcg atcaaaatat ccgcacggcg gcaatgcgcc
     2101 ttgatgtcgc gcgtctgtga atgacaaatc gtcaccgtcg cgttcgcgtg ttttgctttc
     2161 tggcaaagaa tggcggccat cggtttcccg acaatgttgc cacgtccaag aatcactgct
     2221 tccgcgcctt caatttttgt ttgcgagcga accagcaatt ccaaaatgcc ggccggtgtg
     2281 catgggacga accccgttgt gtcaccaagc attaatttgc cgacattgaa gggactgaaa
     2341 ccatccacgt ctttccaggg taaaaccttg gagtaaattt ccgtctcgcg gatatgtttt
     2401 ggcaatggcg cttgaatcaa aattccgtga atacgaaagt ctttgttaag ctggcgcacc
     2461 gcctcaacca gttcgttttc cgttgtgctt tcgggcagca cgcgggtttc ggaaaaaatg
     2521 ccgagttgcg cgcacatttt ttccttcatg ccgacatata cgcgcgaggc tgggtcttcg
     2581 ccgacgcgca caaatgccag tccgggctgg acaccgcggc ttttaaggac ctcaatccga
     2641 cgggcggttt cgccctggat ttgacccgcg atggcgcggc cgtcaatgag gtctgcggac
     2701 atccgtcact tcacaacttg tatcttctgg atggtcagga cgggagtgtt gggccagcgt
     2761 tcgtccaatt cttcctcgcc ggtgacgaga acggtcaagc ccctgtaacg ggccagcgac
     2821 aggtttgtcg aagtggtata gagataatcc attatggtac cgtcatcgag gctcttcaat
     2881 tcataatgcg acggcgcctg gatgctcaca gatccgccca caagaccttc acgctcgatg
     2941 atgcgcggcg ggggcggttc ttcaaccgca ggagctggtg gaggcgcaat cggcggcagt
     3001 ggaacattcg gcggcggtgg cgcaacactg cccggtgttt ccgtcgatgg cggcgcaact
     3061 ggcggcggat tgccaacggc gacttgcgtc ggcggaggat tggtgggtgc ggcaggttcg
     3121 ggggctttat gcgtcagcaa atgcgcggcg acgaatgcaa atgcgttcgt gggcgcttca
     3181 atctgcgtcc aatcaccttt cgtattgccg gcggtcttga cggcatcacc tttttgcaac
     3241 gtgccgatga cgctatagtt ctcgccgggg ccggtgcgca aattcaactt gcgcgcggta
     3301 acggtctggt tggtggcatc aatgaaaggt gcgtttaccc aaacgtgcag tgtgccgggc
     3361 aaggcgatct tcgcccactt ggaaggctcg tcggtctttg gatgcttgag cgtcacttct
     3421 tccaaaacgg tgacggtatc gcccttctgt aaatggccga cgacttcgct gttgatgtgg
     3481 gcttggccgc gaatgttcac gttgttctgc ttcgcaacgg ctggctcgcc cggaacgagc
     3541 ggctcggcag gcatttccgg cgcggcagcc ttggcttctt ttgttgtttt ctttttggcg
     3601 gttttctttt tcgtcttctt cttggcaggc gcgttggtcg tggtgcccgc gtcgggcggc
     3661 gtcaacggtg cgacagatgg cggcggtgtt gccggttgcg tggaagtgtc cggcgcggca
     3721 ggaagcggcg gcggagtgct gggtgatgga ttgccgccgg gcggaggcgt ctgcgccagc
     3781 aaagctgccg aaaccatggt tgccaaaatc aatccaaatt tctttttcat aatgtcttac
     3841 ccggagagta gagatttaat ttcggtttct gtcaatgtcc gccatttgcc gccgggcaac
     3901 tcgcccagct tgattttgcc gatctgcgtc cgcacgaggc ggtttacctc cagcccctgc
     3961 gattcaaaaa gccgccgaac ttcgcggttt tttccctcgg ccagctccaa ttccaccacg
     4021 ctgttggaat tgttcgccga gagcaggcgg gctttttccg ccttgagcat ctcgccttcg
     4081 tgcgaaacgc cgcgcgtaat acggcccaac atttccggct gcacccggcc ggtcacggtg
     4141 gcgatatatt ttttccgaac gccgtaacgc ggatgcgtca ggcgcaggct aaactcgccg
     4201 tcgttcgtca aaaaaatcag cccttcgctt tccgcatcga gccgtccgac ggagtaaagg
     4261 tttccccact ccttcggcaa caggctgccg atcgtgcggc gttcccgttc atcgagccgt
     4321 gtgcagatat acccggaagg cttgttcaat gccacataaa gcttacgctt cgccctcacc
     4381 atccggccat ccaccgccac ttggtcatga acgggatcca ccttgccgcc caattcattc
     4441 actgtgcggc cattcaccga gacgcgccct tcgagaatga ttttctcgct ggcgcggcgc
     4501 gaagcgatgc cggcttccgc caaaaatttt tgcaaacgaa ccatccaggt gcggagtgcg
     4561 gaatggaccg agggtggatt gccaatcgga ttattccgca atccgcactc ccgactccga
     4621 attcaaatca cgcttccggc ttggttttgc gcaggccggt gatgcgctcg ccgtccttcc
     4681 attgaccgcg tttcttgagc aggccgaccc gctcgaagcg tttgagtacg ttgcgtttgc
     4741 cacccatggt ggcgacggcg cgcagactgc gatgttgtga cataaatgat cctgacgtta
     4801 aaattctccg ccttccattg aaagcgaacg gtgtttctaa cacgtctttg gcggcttgcc
     4861 aatggctaat ttccacaggt taaatggtta aatcggtcaa agggcatgga acaaattcaa
     4921 acgatttttg tgattttgta acaaatccaa gaattcaatc gtgtcaccag ttcatgtaat
     4981 actttttcac aaacatttaa tttgagaagt ccctttccgt gaagaacatt atgccacaag
     5041 tcagaatcct gattcagtga acccgtttgg aaatgagcaa gcaagcgaaa gataattttc
     5101 tggtgctggt cgcggacgat tccgagagcg accgcatctt gctgaagaag gcgatggagc
     5161 gcgccacaac gttgcgcatc atcggcgaag ttgccaatgg ccacgatgtc atctcctacc
     5221 tcaagggaca tgcgcatttc aatgatcgtg aaaaatatcc gttgcccgat ttattgctgc
     5281 tcgatttgaa aatgccgctc aaggacggct ttgaagtcct cacatggctt cgcacgcaat
     5341 ccttccaaaa tttgaccgtc gtggtgctga ccgactcgat gcatgccgag cacatcaagc
     5401 gcgcgctgga cttgggcgct gatttgttcc aggtgaaacc aaagatgccg cacgacctca
     5461 acgcgatgat cctcgcattg gaagagcacc tgcaactttt cgcgcgcaca ccgacgcgcc
     5521 gcacgcatct tcccgcggca atgcgattgt aactatttcg cgggttaata aacttccctt
     5581 cttatttcga agtcttgggc aaggcgacga cccattgcaa ggtgatcttc acgttcgggc
     5641 gcgtctcaaa aatgccgaac tttgtcggag gtggcacctt gtaatcggtc atgttgattt
     5701 caactggccc gccactaatt ttcaacttgg tcttgtctat tgtctcgatg ctcacgggca
     5761 tggtgatggg tttggtgacg ccgttgacgg taaggtcgcc tttggtgtcg aattgaaatg
     5821 gcgtgcccgc gctgtgcggc tgcttaaggg tcatttcgct caacttgaaa gcgatttgtg
     5881 gatggtccgt cgcattcatc gcctgttgca tgacctggtc cattcccgac gttccacttt
     5941 gcaatgtaac caccggaatg tttgcttcgg ctttggcatt gagcttgtcg ccggacatgc
     6001 ccccgatggc agcttgcgag gaatcaatcg tcacattggc gggaacttca aaccaaccgc
     6061 caatgatagt gccgtccatg gtccaatcat ggatattggc tgagccgtcc aagtggacct
     6121 ttgcgccgcg gggacgcgcc ctgtaacgga cgaaatcctc ggcgtttgag ttggtggctg
     6181 ccagcatgga gcagccaatc gcgacggcaa ataaacggag tgtcaggttc ttcataattt
     6241 cccttgtgat caaatatcaa acagttcttt cggcaacatc accttctcgc gcttcttgat
     6301 ggcttcgttc gccttgatgg cgatgacagt tgcatcgaag ccctccctgt aaccggcggc
     6361 gggcaggcgt cctttggcat tttcgggctt ggccatctca tcgagaaaac cttccgcatc
     6421 ggctccgtac gcatcagtga acgccttcgc atgggtcgcc gtaaaaagcg aattatgcgc
     6481 gaaggccttc aacgcgtaat gcagcgtcga ttcctggtaa ggcgaagcct cgatcccctt
     6541 cttcccgctg gtcgagtgcg acgcgcccgc ggaaagtgtg atgccgactt ccttgtggaa
     6601 ctgttccttg gcagcataga cttcccagcc gaagacctgc gagtccactt cctcgaacaa
     6661 ccacgccttg gccggcgtgt aattctggcc aagcgattca gggcggaaca aaatcgccgc
     6721 gcccgttcca taaatcatgt cgtattcgcc atcgaaagaa ttcgccagcg tgcagtcgta
     6781 cattgcattc accttgcgcg gatattccat gatcgtctgg atggtatcgg ccacatcgcg
     6841 gtcgtcgcct gaatcttgta tcttgggatc gagaatggag ccgaagccgg tgacagcgag
     6901 cggttttttt ccgagcatcc acgtcacgag atcatattga tgcacgccga tttcgccggc
     6961 cagaccggcg gaagtttccg aataaagccg ccagttgacc tccttgtcgc gttcggacgt
     7021 ggacccggcc ttgcgccagc tttcgcgctt gtgccattgc gagcgcgcca tgacgggcgt
     7081 gccgaccgcg ccggtgtgga cgaaatccag cacgaatttc ttgccagggt ccgagcgcat
     7141 ctgcaggccg gattgaaaat tcaacttctc cgccgccttc gccgccttgg caatcgcccg
     7201 cgcgtcctca acagttgtgg ccaacggcgc ttcacaatag acatgtttgc cggcctgcaa
     7261 tgccgcaatc acgatgtccc gatgcagatg cgaaggcgtg gcgacgacaa cgcattcgac
     7321 gtccttttgc tcaagcagct ttttataatc gtcataaact tgcgcattgg gcgcgtagtc
     7381 tttgaccctt ttgatcgccg gcttgtagtt atcgcatatc gcgacgacgg gagcattcgg
     7441 tggatttgga gttccatcct tccgcgttcc accgggcaac agtgcgagcg tctggagaat
     7501 ttccttgccg cgtgtgccgc aaccgatgac agcaaaattg attggctggt cctctgtgct
     7561 gaaatcagtt tccgcctgtt tcggtgtcgc atcgtccgca taaagcggcg tgccgcccat
     7621 cagcaacatg aggctgccca aggaagcggt gctcttgatg aaatcgcgcc ggttgaaatc
     7681 gttctggcct tgattggctt cgttttcgct cataattagt ccggctattt aatcaattga
     7741 ccctgcaaac tcaaatctaa tttatacggg aacgtatcag cctgtacgcg attttcaatc
     7801 cgctcctcca atttttgaag gctccaagcc gagcgcccat ttgatgccgc cgagcaaatg
     7861 cagttgaaaa tcgggatttt cccagaccgc atcttcatgc cccagggcgg tgtaaaaaac
     7921 tttccctccg ccatacgatt tgcaccagga gatgggaaaa ttgccgggcg tttcagaacc
     7981 gggcaccttg tcgagccgca acaacagatg cactttgtcc ggcgaaaaat ttttcatcat
     8041 gtaaatctcg tcgaacacgg gaaaaacttt tccgaaatgc cgggtcgaag gatgtttcgg
     8101 gtccaccacg atgcaatcca ctttggcgac ggtgtgttca acaaactcac cgccaatcat
     8161 ttcaatgtaa ggatcaacgc cattctcgcc gtgaaaagta tccgtcgccg aatgt
//
LOCUS       DASZMM010000019         5515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3578629, whole genome shotgun sequence.
ACCESSION   DASZMM010000019 DASZMM010000000
VERSION     DASZMM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3578629"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VG754_00460"
     CDS             complement(<1..428)
                     /locus_tag="VG754_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17703.1"
                     /translation="MSAITVAYIFERYPVLTQTFLRRELAGLSRAGLRLEVHSMLDAP
                     IRSGHVPDELPGYIPVFHFRWWHASKLFIALPREFLRDPKLLADGWRLWREHRFKDRE
                     NFWATLWAVIFAICQAKKFRERKPDVLHGVRATGPATAAA"
     gene            complement(425..1492)
                     /locus_tag="VG754_00465"
     CDS             complement(425..1492)
                     /locus_tag="VG754_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HWF17704.1"
                     /translation="MARVLYGVMGNTHGHIARTLAIVTRLPEHEFHFVGGGRVPELLG
                     KKFPVLEVPVKRTIHKNQRVSIPATIGQIARCAIKFPSTQRQILDLIHRWQPDIAICD
                     REIFLPHAARAAGLECVSLDHSHILQACRYPVPRGQRVSWTLARLEDTLFFNLTQRNL
                     IVSFFHPELKPSGKTINELLPPVLRPEIQQVHSSDGEHVLIYQTSPTFTALLDAARQL
                     SRRIIVYGFRNEHAVEGNITFKPFHPVSILEDLATCAYAVVNGGHNLICEALALNKPL
                     LCFPITGQFEQYINAWHVRAKGCGDFSTSRQPPPSLFAQFEKRLDEYRQNIRANFVDG
                     TDAVISRVRELISKQASRKTA"
     gene            1578..2057
                     /locus_tag="VG754_00470"
     CDS             1578..2057
                     /locus_tag="VG754_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17705.1"
                     /translation="MREYRRIRKIISWFLAAYFAGGLLTLMRPEMEVFPVYSWFLFAL
                     VPQSGPQYGLLLHNVNGRSIEPPRLYQEAGDWISTSHSVTAFKLTQELGAAVEKNLPE
                     KLRDRELLEKNWLPPRTSYELIKLNADPITRWKTGKYEITQTLGTFANADGESEKNR"
     gene            2054..3103
                     /locus_tag="VG754_00475"
     CDS             2054..3103
                     /locus_tag="VG754_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17706.1"
                     /translation="MKTHADNALEFPSSFSQKKVELSGHAEKATSKIISPPQSQKNGK
                     QNWASAIRFQPRAFMRARLLVRCYYACGLYFALALMPDWAGLLERNMPDPLWPVAWLA
                     HVPLRAGIAAILVLYLLGSMGAAIWPQKRWARTIGFLGLFQWVAFNNSFGKIGHSMHL
                     WVLTSAALIFLPQAEAWGGSPTRAQRQKFLLIFWGCQAAVMLVYSMSGLGKVLGAIYQ
                     LCTGQTQAFMPGALATVVAERLLETNSRSLLGPWLIEHPSAGWPLMVANIYLQLFAFP
                     AVFRPSLHRVWGIGLILFHIGSFLLLTISFPQNALLLALFFVNSPFRRENENWRQTLA
                     DLPLLGRFFPRRCAS"
     gene            3091..4233
                     /locus_tag="VG754_00480"
     CDS             3091..4233
                     /locus_tag="VG754_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HWF17707.1"
                     /translation="MRVLILSSSTGGGHDMRARAFQAWAQLAPELNLEAQVHRPLDEG
                     PGLYRFGVGLYNWIQKSAPRLHHVYFNFLEVAAPCRSARGLLGAKQFCETLDRMRPDV
                     LLSVHGTLNHGYFDCARNFLGRDKVRCVTYCGELFGGYGFSRNWVNPDADLFIGAVPE
                     TCVEAARFGMPTDKNCVGGFLLRPEFYDLAPDDAKRRDHIRNQLGFNPDEFILLLSAS
                     SRGANNHIIFLEAMRGGNLDVQTVVLCGKSVETEARIANWQKQNPKSRVRILPHDTNV
                     AMLLRSVSAVVARPGTGATSEAILSQCPLLLNCLGGAMPQECITVKFCRKHQLAETIQ
                     RPADLARLVSTWTKNPEICAGVRQRMLAACPPGHPRDILRRVAGLA"
     gene            4243..4992
                     /locus_tag="VG754_00485"
     CDS             4243..4992
                     /locus_tag="VG754_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17708.1"
                     /translation="MNAAETQDEIAARLYLHAVLPAFEELGRVSPRAQALIADWHCAV
                     EFAVCGGDGAAVRFQGGTVSVLQERCADAAISLFFFSTSQLVKQFEGRFSLPLPRRGI
                     FRLASLYRFHRLGIILQEELEQHPRLLLGVALASLQPLIAHDHHARELMRDCPEGMAE
                     LCIPNDKLYGWVEWRDGKIHWGRGHAPRVADVVVTFRDEQTALAALRGELDEWAAIGK
                     GELKLRGLMPLAETLGLVMARADHYLERRTK"
     gene            4998..>5515
                     /locus_tag="VG754_00490"
     CDS             4998..>5515
                     /locus_tag="VG754_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWF17709.1"
                     /translation="MTEPHFPKSMEWFERASRVIPCGIYGHAAPAAAVPLEFPYYAAR
                     AEGCRYWDVDGREYLDFLCSYGPVVLGHHHPEVEEAAERQRKLGDCFNHPTPVMVELC
                     ERLVELVDFADWAVLGKNGSDMTTWAVQLAREFTGRKKILRARQSYHGSHPWCTPGHG
                     GLIEEDRIHVHD"
BASE COUNT         1152 a         1340 c         1763 g         1260 t
ORIGIN      
        1 atcgccgcag ccgtcgcagg cccggtggcg cgaacgccgt gcaggacatc cggcttgcgt
       61 tcacgaaatt tttttgcctg acagatggca aagatgaccg cccaaagtgt tgcccaaaaa
      121 ttctcccgat ctttgaaacg atgctcgcgc cacaaacgcc agccgtcggc gagcaatttt
      181 ggatcgcgca gaaattcgcg aggcaacgcg ataaataatt tcgacgcgtg ccaccatcga
      241 aaatggaaaa caggaatgta accgggcagc tcgtcaggga catgcccgga gcgaatcgga
      301 gcatccagca tggaatggac ttcaaggcgc agaccggcgc gtgaaagccc ggccagttcg
      361 cgccgcaaaa aagtctgcgt caacaccggg taccgctcga aaatataggc gacggtgatg
      421 gcgctcatgc ggtttttcgc gaggcttgct tgctgatgag ttcacggacg cgggagatga
      481 cggcgtccgt gccgtccacg aaatttgcgc ggatgttttg gcggtattca tcgagacgtt
      541 tttcaaattg ggcaaacaac gaaggcggag gctggcggct ggtcgagaaa tcgccgcagc
      601 ccttggcgcg aacgtgccag gcattgatgt attgttcgaa ctggccggtg atgggaaaac
      661 aaagcagcgg tttgttcaac gccagcgctt cacaaatcaa attgtgcccg ccattgacaa
      721 cggcgtaggc gcaggttgca agatcttcga gaatggagac tggatggaac ggtttgaagg
      781 tgatgtttcc ttcaacggca tgctcgtttc gaaaaccata tacaatgatg cggcgcgaaa
      841 gctgccgggc ggcatcgagc aacgcggtga aggtcgggct ggtttgataa atgagcacgt
      901 gctcgccgtc ggaggaatgg acttgttgga tctcgggacg caacaccggc ggcaggagtt
      961 cgttgatggt tttgccgctg ggtttcagtt ccggatggaa aaatgaaacg ataagattgc
     1021 gttgggttag attaaagaac agcgtatctt ccaaccgcgc cagtgtccaa gaaacgcgtt
     1081 gcccgcgcgg aacgggataa cggcacgcct gaaggatgtg cgaatgatcg agcgagacgc
     1141 attccaggcc ggcggcgcgc gcggcgtggg gaagaaaaat ttcgcggtcg cagatggcga
     1201 tgtcgggttg ccagcggtgg atcaaatcga ggatttgtct ttgggttgac ggaaatttga
     1261 tggcgcaacg ggcaatttgg ccgatggtgg cgggaatgga gacgcgctgg tttttgtgga
     1321 tggtgcgttt caccggcact tcgaggacgg gaaatttttt gccgagcagt tcgggaacgc
     1381 gtccgccgcc gacgaagtgg aattcgtgtt cgggaaggcg ggtgacgatc gccagcgtgc
     1441 gcgcgatgtg cccgtgggtg ttgcccatca caccgtataa tactcgtgcc atggtggacc
     1501 gtgccgggcg caacagctca tttaatgcat gaccctgacg cggggcttct ttataatgga
     1561 gccaaataga ttcgtccatg cgagaatacc gccgcatacg caaaatcatc tcgtggttcc
     1621 tggccgccta cttcgcgggc gggctgttga ccttgatgcg cccggagatg gaagtgttcc
     1681 cggtctattc ctggtttttg ttcgcgctgg tgccgcaatc ggggccgcaa tatggacttc
     1741 tcctgcacaa cgtgaatgga cgcagcatcg aaccgccgcg gctttaccag gaagcgggcg
     1801 attggatttc cacgtcgcac agtgtcacgg cgttcaagct cacgcaagaa ctcggcgcgg
     1861 cagtcgaaaa gaatttgccg gaaaaattgc gggatcgtga gttgctggaa aaaaactggc
     1921 tgccgccgcg aacgagttac gagttgatca aactcaatgc cgatccaatc actcgctgga
     1981 aaaccgggaa atatgaaatc acgcagacgt tgggaacatt tgcgaatgcg gacggagaat
     2041 cggagaaaaa ccgatgaaga cgcatgccga taacgcgctt gaatttccgt cttcgttttc
     2101 acaaaagaag gtcgagttaa gcggccatgc agagaaggcg acgagcaaga ttatttcacc
     2161 gcctcaatcg caaaagaacg gaaaacaaaa ttgggcgtcg gcgatccgct ttcaaccgag
     2221 agcgtttatg cgggcgcggc tgctggtgcg gtgttattac gcatgtggtt tgtatttcgc
     2281 gctggcgttg atgccggatt gggcgggatt attggaacgc aacatgcccg atccgctttg
     2341 gcctgtggca tggctggcgc atgttccttt gcgcgcaggc atcgcggcga ttcttgtttt
     2401 atatctgctc ggctcgatgg gagcggcgat ttggccgcag aaaagatggg cgcggaccat
     2461 cggatttctc ggactttttc aatgggtggc attcaataat tcttttggca aaatcggcca
     2521 cagcatgcac ctgtgggttt tgacttccgc agctttgatt tttctgccgc aagcggaagc
     2581 gtggggcgga tcgccgacac gcgcgcaacg gcaaaaattc ctcctgattt tttggggatg
     2641 ccaggcggcg gtgatgctgg tctattcgat gtccggactc ggcaaggtgc tcggcgcgat
     2701 ttatcaactt tgcacgggac aaacgcaggc gttcatgcca ggcgcgctgg cgacggttgt
     2761 cgccgaacgc ttgctggaaa ccaactcgcg aagtttgctg ggcccgtggt tgattgagca
     2821 cccgtccgct ggatggccgt tgatggtggc gaacatttac ctgcaattgt ttgcatttcc
     2881 cgcggtcttt cgtccctcgt tgcatagagt gtggggaatc ggtttgattc tgtttcacat
     2941 cggctcgttt cttttgctga cgatcagctt tccgcaaaac gcattgcttc tggcgctgtt
     3001 ttttgtcaat tcgccgttcc gccgcgaaaa tgaaaactgg cggcaaacgc tcgctgattt
     3061 gccattgctg ggccggttct ttccacgacg atgcgcgtcc tgatcttgag ttcaagcacg
     3121 ggcggcggcc acgacatgcg cgcgcgcgcc tttcaggcat gggcgcagct cgccccggag
     3181 ttgaatttgg aggcgcaggt ccaccggcca ttggatgaag ggcccgggtt gtatcggttc
     3241 ggcgtcggtc tttataattg gatacaaaag agcgcgccac ggttgcatca tgtctatttc
     3301 aattttttgg aagtcgcggc gccgtgccgt tcggcgcgag gtctgcttgg cgcgaaacaa
     3361 ttttgtgaaa cgctcgatag aatgcgcccg gatgtgttgc tgagtgttca tggcacgctg
     3421 aaccacgggt attttgattg cgcgaggaat tttctgggaa gagataaagt gcgttgtgtc
     3481 acttattgcg gcgaactttt cggcggatac gggttcagcc ggaattgggt gaacccggac
     3541 gctgatttgt tcattggggc agtgccggaa acttgcgtgg aagcagcgcg ttttggaatg
     3601 ccgacggata aaaactgcgt cggtggattt ttgttgcgac ctgaatttta tgatctggcg
     3661 cctgatgatg ccaagcgtcg cgatcacatc cgcaaccaac ttggttttaa tccggatgaa
     3721 ttcatcctgc tgctgagcgc atccagccgt ggcgcgaaca atcatataat ttttttggaa
     3781 gcaatgaggg gtggcaatct ggatgttcag acggttgttt tgtgcgggaa atcggttgaa
     3841 accgaagcgc gcattgcgaa ttggcagaag caaaatccca aatcgcgcgt gcgcattttg
     3901 ccgcatgaca cgaatgtcgc gatgttgttg cgcagcgttt ccgccgtggt tgcgcggcct
     3961 ggcaccggcg caacgagcga ggcgattctc agccaatgtc cgttgctgct gaattgtctt
     4021 ggaggcgcga tgccgcagga atgcatcacc gtgaagtttt gccgaaaaca tcaactggcc
     4081 gaaacgattc agcgtccggc cgatttagcg cgcctcgttt cgacatggac gaaaaatccc
     4141 gaaatttgcg cgggggtccg ccaacgaatg cttgcggcgt gtccgcccgg acatccgagg
     4201 gacatcttgc gccgtgtggc cgggctcgcg taggctgatt ggatgaatgc ggcggaaaca
     4261 caggatgaaa tcgcggcgcg gctctatttg cacgccgtgt tgcccgcgtt tgaagagctg
     4321 ggccgcgtct cgccgcgcgc gcaagcgttg atcgcagact ggcattgcgc ggtggaattt
     4381 gcggtctgcg gcggtgacgg cgcggcggtg cgatttcagg gcggaactgt ttcggtgctg
     4441 caggagcggt gcgctgatgc cgcgatctcg ctattctttt tttccacgag ccaattggtg
     4501 aagcagtttg agggccgatt cagcctgccg ttgccgcgac gcggaatttt tcggctggcg
     4561 agtttgtatc ggttccatcg gctcggaatc attttgcagg aagaactgga gcagcatccg
     4621 cgattgctgt tgggcgttgc gctggcgtcg ttgcaacctc tgatcgctca tgaccaccat
     4681 gcgcgggaat tgatgcgcga ttgtcccgag ggaatggcgg agttgtgcat tccgaatgac
     4741 aagctttatg gctgggttga atggcgggac ggaaaaatcc attggggacg cggccatgca
     4801 ccgcgagttg ccgacgtggt ggtgacattc cgtgatgaac aaacggcgct ggcggccttg
     4861 cgcggtgagt tggacgaatg ggcagccatc ggcaaaggtg aattgaaatt gcgcggactg
     4921 atgccgctgg cggaaacgct tgggttggtg atggcgcggg cggaccatta tctcgaacgc
     4981 cgcacgaaat aatcgaaatg accgaaccgc attttccaaa atccatggaa tggttcgagc
     5041 gggcgagccg cgtgattcca tgcgggattt acggtcatgc cgcgccggcg gcagcggtgc
     5101 cgttggaatt tccttactat gcagcgcggg cggaaggctg ccggtattgg gacgtggacg
     5161 ggcgcgagta tctcgatttt ctttgcagtt acgggccggt tgttttggga catcatcatc
     5221 cggaagtgga ggaagcggcc gagcgacaaa ggaaattggg cgattgtttc aatcatccca
     5281 cgccggtcat ggtggaactt tgtgagcggc tggtggagtt ggtggatttt gcagattggg
     5341 cggtgttggg aaaaaacggc tcggacatga cgacgtgggc ggtgcagttg gcgcgggaat
     5401 tcacgggaag aaaaaaaatc ctgcgcgcgc ggcagagtta ccacggttcg catccgtggt
     5461 gcacgcccgg tcacggcggg ttgatcgagg aggatcgaat tcatgtgcat gattt
//
LOCUS       DASZMM010000020         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1386128, whole genome shotgun sequence.
ACCESSION   DASZMM010000020 DASZMM010000000
VERSION     DASZMM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386128"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VG754_00495"
     CDS             complement(<1..338)
                     /locus_tag="VG754_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17710.1"
                     /translation="MTGTRVIAMVLCAAIVGGFSVYIARQRGIDRVHAENAMMKRQLD
                     QARLERQAAVEMAATREAELEKVRQDSLALAKLREQMKSNAAPSPPTGFYIGTDQPVY
                     VTYGTSNAVFR"
     gene            complement(363..1244)
                     /locus_tag="VG754_00500"
     CDS             complement(363..1244)
                     /locus_tag="VG754_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HWF17711.1"
                     /translation="MKPRLASIGFKYSAAIAVILMALLLWQAYGQMRGWGRGRRMQAR
                     NPEEVRENELMEKAINPAFKEDVFTFARLKFTAERGFGYGGGRLWDDDSPEADLNVIF
                     RLYETTSMKVRPGVNVVDITTNDLKNYPFVYVAAAGRLDLSDEDAACLRQYMLSGGFI
                     MADDFWGDDQWQHFYEQFKRIFPKREPVELTLDHPIFRTVFKFKKQPQIPSVGAFMQY
                     GVSYDPGWPYENKNNEPHYYAVYDDKQHMMMLICHNNHYGDGWEHEGDDESYFDTFSE
                     PQAYPMLFNVLVYAMSH"
     gene            1422..>3104
                     /locus_tag="VG754_00505"
     CDS             1422..>3104
                     /locus_tag="VG754_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF17712.1"
                     /translation="MGTSLLVIVPGAVAQTFEETQQDYRHGDYEKVIKTASKKIEDYD
                     YGGDWRLLLIKTLLTVGRYHEAHTNALAALHDYSDGLEKHLLARETYLYENDAESANR
                     QLMEIRLLIERRSGRFQTEDIVPLGRALLMLGVEPRLVLDNCFRRAETMSPPQREAFL
                     ASGQLALDKHDYALAADAFRAGLKQFADDPDLEAGLAQAFEPSDREEMSMAIHAALEV
                     NPRHIPSLLLLADHLIDAEEYEDAEKQLSLVLKVNPHNPEALAYRAVLAHLRNDTAGE
                     ENFRANALQFWKNNPRVDYIIGLKLAQKYRFSEGAAAQKRALAFDPTYLPAQRELAED
                     LLRLGQSDEGWKLVHQVHASDNYDVAIYNLITLHDQMAKYQTLTNSDFIVHMTPQEAR
                     LYGDSVLELLGRAKATLCKKYGIELTQPTTVDIFPEQKDFAVRTFGLPGNPGYLGVCF
                     GSVITANSPASQAPNPANWQDVLWHEFCHVVTLNATKNRMPRWISEGISVYEERQASP
                     AWGANMDLANRAAILKGKLTPLGELSSAFLTPKNGEQLQFAYYESSLVVEFLV"
BASE COUNT          695 a          881 c          844 g          684 t
ORIGIN      
        1 cgaaatacgg cattcgatgt gccgtaggtg acatacaccg gctggtcggt cccgatgtaa
       61 aatcctgtgg gaggagaagg cgcggcattg gatttcatct gctcgcgcaa ttttgccagc
      121 gccaacgaat cctggcggac cttttccaat tcagcttcac gcgtagcggc catttccacc
      181 gccgcttgcc gctccaaacg cgcctgatcc agttgccgtt tcatcattgc attttctgca
      241 tgaacccggt caatgccccg ttgccgcgca atgtaaacgg aaaacccgcc gacaatggcc
      301 gcgcaaagaa ccatggcgat cacccttgtg ccggtcatgc ggttcccggg tctgacagcc
      361 cgttaatgtg acatcgcata gaccaggaca ttgaacaaca tcgggtaggc ttgtggttcg
      421 gagaacgtgt cgaaataact ttcgtcgtcg ccttcatgct cccagccgtc gccataatga
      481 ttattgtggc agatgagcat catcatgtgc tgcttgtcat cgtacacggc gtagtaatgc
      541 ggctcgttgt tcttgttttc atacggccag ccgggatcgt aggagacgcc gtattgcatg
      601 aacgcgccga cgctcgggat ttgcggctgc tttttgaatt tgaacacggt ccggaaaatc
      661 ggatggtcga gggtcaactc gacaggctcg cgttttggaa aaatccgttt gaactgctcg
      721 taaaaatgct gccattggtc gtcgccccaa aaatcatcgg ccatgatgaa cccgccgctc
      781 aacatatatt gacgcagaca agccgcatct tcgtctgaca aatccaatct gccggcagcg
      841 gccacgtaaa caaaaggata gttcttcaga tcgttcgtgg tgatgtccac aacattcacg
      901 ccgggacgga ctttcatgct ggtggtttca tacaggcgaa aaatcacgtt caagtccgct
      961 tccggactgt cgtcgtccca taaccgcccg ccgccgtatc caaatccgcg ttccgcggtg
     1021 aatttcagcc gggcgaacgt gaacacatcc tccttgaacg cgggattgat cgccttctcc
     1081 atcaattcat tttcccggac ttcttccgga ttgcgggcct gcattcgacg gccgcgcccc
     1141 caaccgcgca tttggccgta agcctgccac aacaacaatg ccatgagaat gaccgcgatt
     1201 gcggcggaat acttgaaacc gatgctggcc agtcttggtt tcatgttcga gacaaggcac
     1261 tgtctgtttc acagagttta ctttgagggc gcggaaatgc aagctgcgcg tccgattgtc
     1321 cattgattcg gggcacgagt cggcgtatga atgatggaac gatgccgcgt cacaagctgt
     1381 ttttgtcagt ggctctgcta tggcccattg gcctgtggtt gatggggaca agccttctcg
     1441 tcatcgttcc cggcgcggtg gcgcaaacct tcgaagaaac ccagcaggat tatcgtcacg
     1501 gcgattatga aaaagtcatc aaaaccgcct caaaaaagat cgaggactac gattacggag
     1561 gcgattggcg gttgctgctg ataaaaactt tgctgacggt cgggcgttac cacgaggcgc
     1621 acaccaacgc cctcgccgcg cttcatgatt attccgacgg tttggaaaaa cacctgctcg
     1681 cgcgggaaac ttatctttac gaaaacgacg ccgagagcgc gaaccgtcag ttgatggaaa
     1741 tacgcctgct catcgagcgg cgttccgggc gcttccaaac cgaggacatc gttccgctgg
     1801 gccgcgcgct gttgatgctc ggcgtcgaac cgcgtctggt tttggataat tgcttccggc
     1861 gcgccgaaac gatgtcgccg ccgcagcgcg aagcttttct ggcgagcggc caacttgcat
     1921 tggacaaaca tgattacgca ctcgccgccg acgcgttccg ggccggattg aaacaattcg
     1981 cggacgaccc cgacctggaa gccgggctgg cccaagcctt cgaaccaagc gaccgcgagg
     2041 aaatgtcaat ggccatccat gccgcgctcg aagtaaatcc gcggcacatt ccgagcctgt
     2101 tgctgctggc cgaccatttg attgacgccg aggaatacga ggatgcggaa aagcagcttt
     2161 cgcttgtgct caaggtgaat ccgcacaatc cggaagcgct ggcgtatcgc gctgtcctgg
     2221 cgcatttgcg caatgacacg gcaggcgagg agaactttcg cgccaacgcg ttgcaatttt
     2281 ggaagaacaa tccgcgcgtg gattatatca tcggactcaa gctcgcgcag aagtatcgct
     2341 tttccgaagg cgcagcggcg caaaagcgcg cgttggcgtt cgatccgact tatttacccg
     2401 cgcaacgcga actggccgag gacttgttgc gtttgggcca atcggatgaa ggctggaagt
     2461 tggtccatca agttcacgcg agcgacaatt acgacgtcgc catctataat ctcatcacgc
     2521 tgcacgacca gatggcaaaa tatcagacac tgaccaactc ggatttcatc gttcacatga
     2581 cgccgcagga agcgcggctt tacggcgatt ctgtgctcga attgctcggc cgcgcgaaag
     2641 ccacgttgtg caaaaaatac ggaatcgaac tcacgcaacc gaccacggtg gacatttttc
     2701 cagagcaaaa agatttcgcg gtgcgcacgt ttggtttgcc cggcaatccc ggttatctcg
     2761 gcgtctgctt cggttccgtc atcaccgcga acagtcccgc ctcgcaagcg ccgaatcccg
     2821 ccaactggca ggatgttctc tggcacgagt tctgtcacgt ggtcacgttg aacgcgacga
     2881 aaaatcggat gccgcgctgg attagcgaag gcatttccgt ttacgaagag cggcaggcca
     2941 gtcccgcctg gggcgcgaat atggacctcg ccaatcgcgc ggcgattctg aaaggaaagc
     3001 tgacgccgct gggcgaattg agcagcgcat ttctcacacc caaaaacggc gagcaacttc
     3061 aattcgccta ttatgaatca tccctggtcg tcgaattttt ggtg
//
LOCUS       DASZMM010000021         5638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4953356, whole genome shotgun sequence.
ACCESSION   DASZMM010000021 DASZMM010000000
VERSION     DASZMM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4953356"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VG754_00510"
     CDS             <1..849
                     /locus_tag="VG754_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWF17713.1"
                     /translation="ASPQHPESIAAYATWAAAAVKHFHGRHIIWEIWNEPNGFFWKPK
                     PDAEQYSALAVAAGKAIREADPRATLIGPALSGFQPPYMETFLKSGVLRYLDAVSVHP
                     YRSAKKWPETAEGDYQKLREKIEQYGQTEKQKKMPIISGEWGYSTKIGDVSPETQAAY
                     IVRQQLFNLLNGVPLSIWYDWKNDGTDPREVEHNFGTVTDELEPKPAYEAIKVMTHEL
                     EGYRIKKRLETDKNDFVLLCSKPFAKDKIVAWTTGYVHNTTTLITKGKGTQIELGQMP
                     KYLTLK"
     gene            complement(895..1101)
                     /locus_tag="VG754_00515"
     CDS             complement(895..1101)
                     /locus_tag="VG754_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17714.1"
                     /translation="MNNTALAKDRNNWTLLNKQLAQAKTLLRQMRETVADIEDARTIE
                     RVKRANGNKRTIPWQQVKKELHLD"
     gene            complement(1254..2465)
                     /locus_tag="VG754_00520"
     CDS             complement(1254..2465)
                     /locus_tag="VG754_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWF17715.1"
                     /translation="MTNQQPLITLKAQSVTPVGDMVLVPQFQLQRSTDLKIWSNVGSP
                     VTGTPHQTLSMLDTNSGIGFYRAQSIIDEEYAQLDNAVLSSGQLVGADFFGASLFGAN
                     LSSANLQRAVLAGADVRNADLTGADLTAADLFGIEAAGAIFDQGELTNVDCSFGDFGS
                     ASFADTDLGNADMSSAILSGADMDFAIMKGMKLDTNTVIDPKPMLIWHLVNLGASNVV
                     LTNVDLSNAFLENVNFNGIKFNSANLEFSDLGNADLRGANFTNANMLFVAFLGSLINS
                     NTVLDARSLLVWRILNQPATNRNLAGSNLVNVTLVGANLINANLTSAFCDVSELFAAN
                     FNGANASHANFSQSDLGSATFTNANLTSANFSFANLADANFRNAITNGWIISGATFTN
                     TIMPNGSIRNN"
     gene            complement(2609..5506)
                     /locus_tag="VG754_00525"
     CDS             complement(2609..5506)
                     /locus_tag="VG754_00525"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWF17716.1"
                     /translation="MLLAAATLCLLAIPYADAQFTTSNFFNWETPPVHPVALSPDGTK
                     LAVCNLPDDHVEIFDVTSGAPVSLASIAVGLNPVSVCFRTTNELWVANYISSTLSVID
                     LGTMRVFNTINTTNQPSDIVFAGASNLAYVSCGQPNVVQVFNPISSQLVTNLNIDGNR
                     PRAMSVSPDGSKVYVAIFESGNASTIISTGVSDLNNFPKPSPITFPDAPSHGLDPPPN
                     SGTNFVPAINPALTNPPPRTGLIVKKNSAGRWMDDNNGDWTEFIRGTNAAFTGRVPGW
                     DMPDHDLAVINTTNFSISYASGLMNICMATAVNPASGKISVVGTDALNQIRFQSVLDG
                     VFVRVELAQVDPVTLTNTVVDLNPHLTYQTPTVSDAEREMSVGDPRGIVWSSDGTRGY
                     VTGMGSDNLIVIDGQGNRVGTNPAITVGQGPTGMALDERHNRLYVFNRFDDSISTVDT
                     VGQTVVSTLPLFDPTPQVIKSGRPFLYDTHLTSGLGQLSCGSCHVDARMDRLAWDLGD
                     QTGTMDVIDSTFNFANNSPNPTNNFHPMKGPMTTLTLQDIIGHEPFHWRGDREGIEAF
                     GPTFTNLQGMPGGLTTNQMQQFKNFLATVRFAPNPFRQIDNSLSTNLPIPGHFALGRG
                     ALPAGAQLPNGNAVNGMNTFRLITTPDCIVCHTLPTGLGTDMNFITNHWVSLPVSTNN
                     SHHVALIELLRSSELPFKIPSLRNLFDKMGMDLAHTNSRAGFGFLHDGGVDTLVRFLQ
                     DGLSLTNDQQTADIVAFLLSFTGSDLIPGSLTDINRSPGLPSLDTPAGVGKQITVNSS
                     NSVALINTMISMAGNPTNRVELIVKGFKNGAARGWFFNTNGSIFLSDRPGETYTSTAL
                     RALAAIGSEQTYTLVPSGAGRRMGIDRNLDGHLDGELLLQGMSVATNGPSVSCTSVVG
                     LTYQLQFKNNILDTNWSVVPGAVAGTGNKISLIDNTMGTNQTRFYRVTTTE"
BASE COUNT         1308 a         1499 c         1586 g         1245 t
ORIGIN      
        1 gcctcgccgc agcatccgga aagcatcgcg gcttatgcga cgtgggctgc tgccgccgtg
       61 aaacattttc acggccgtca tatcatctgg gaaatctgga acgaaccgaa cggctttttc
      121 tggaaaccaa aaccagacgc cgaacaatac tccgcgctgg ctgtcgccgc tggtaaggcc
      181 attcgcgaag cagaccctcg cgcgactttg attggcccgg cgctttcagg ttttcaaccg
      241 ccctacatgg aaacattttt gaagtctggc gtgctgcgat atctcgatgc cgtgagcgtg
      301 catccttatc gcagcgccaa gaaatggcct gagacagccg aaggcgatta ccagaagttg
      361 cgcgaaaaaa tcgagcaata tgggcaaact gagaaacaga aaaaaatgcc catcatcagc
      421 ggtgaatggg gttattcgac gaagatcggc gatgtctcgc ccgaaacgca ggcggcgtac
      481 atcgtgcgcc agcagttgtt caatttgctg aacggcgtcc cgctttcaat ctggtacgat
      541 tggaaaaatg atggcaccga cccgcgcgaa gtggagcata atttcggcac cgtgacggac
      601 gaactggaac ccaaaccggc ttacgaagcc atcaaggtga tgacgcatga actggagggt
      661 tatcgcatca aaaaacgatt ggaaaccgac aagaatgatt ttgttctgct ctgctccaaa
      721 ccgttcgcaa aagacaaaat cgtcgcgtgg acaaccggct atgtgcataa tacgaccacc
      781 ctcataacta agggaaaagg cacgcaaatc gaactgggcc aaatgcccaa atatttgact
      841 ctgaagtagc atttagccgt ttgtcgcgcg gcgatcaagg aagaggagag tttcttaatc
      901 caaatgcaat tcctttttga cctgctgcca aggaatcgtt cgtttgttgc cattggcgcg
      961 tttgacgcgc tcaatcgtgc gcgcgtcttc aatgtccgca acggtttcgc gcatctgacg
     1021 caacaaagtt ttggcctggg cgagctgctt gttgagcagc gtccaattat ttcggtcctt
     1081 cgccagggcg gtgttattca tatatgtctt ttctatcacc cacatcatat acaatcaggc
     1141 gggagccttc caattcaaac agcacgataa tcgccggctc gaagcctttt taaatagcaa
     1201 gctgccgatg tgcgaactag aaccatttca ccctcgttgt cttcaccgcc ttttcaatta
     1261 ttcctaatcg aaccgttggg catgatggtg ttggtgaaag tcgcgccgga gatgatccaa
     1321 ccgttggtga tggcattgcg gaaattcgca tccgcaagat ttgcgaagct gaaattggcg
     1381 gaggtaagat tcgcgttggt gaatgtcgcg ctgccaaggt cggattggga aaaatttgca
     1441 tgactggcgt tcgcgccgtt gaagttggcc gcgaaaagct ccgacacatc gcaaaaagcg
     1501 gacgtcaggt tggcgttgat gagatttgcg ccgaccaggg tcacgttgac aaggttggag
     1561 ccggcaaggt tccggttggt tgcgggctga ttgaggatgc gccagacgag gagtgatctg
     1621 gcatcgagaa ctgtgttgga attgatgagc gagccgagga acgcgacaaa cagcatgttc
     1681 gcattggtga aattcgcgcc gcgcaagtcc gcgttaccca gatcgctgaa ctccagattc
     1741 gcgctgttga atttgatgcc gttgaagttc acgttttcca gaaacgcgtt gctgaggtcc
     1801 acgttcgtca acaccacgtt ggacgccccc aaatttacga gatgccagat caacatcggt
     1861 ttcggatcaa tcaccgtgtt cgtgtccagc ttcatccctt tcatgatggc aaagtccatg
     1921 tccgcgccgc tcaagatggc cgaactcatg tcggcgttgc caaggtccgt gtccgcaaaa
     1981 cttgccgagc caaaatcgcc aaagctgcag tcaacattgg tcagttcgcc ctggtcgaag
     2041 atcgcgcctg cggcctcgat gccaaacaga tcggctgccg tcaaatccgc gccggttaaa
     2101 tcggcattgc gcacgtccgc gcccgccaaa accgcgcgct gcagatttgc cgaactcaag
     2161 ttcgcgccaa acaaggaagc gccaaaaaaa tccgcaccga cgagctggcc ggacgaaagc
     2221 accgcgttgt ccaactgcgc gtattcctca tcaatgatgg attgcgcccg gtaaaaaccg
     2281 atgcccgaat tcgtgtccaa catgctcaat gtctggtgcg gcgttcctgt gaccgggctg
     2341 ccgacattcg accaaatttt caggtccgtg ctgcgttgca actggaattg cggcacgagc
     2401 accatgtcgc ccaccggggt caccgattgc gccttcaacg tgatgagggg ctgttgattc
     2461 gtcaaggtga tgctgatgct gaaatttctt tgcgcgaaaa cgctgccagg aaaaacaact
     2521 gccaatatcg ccaaaaaagt gagtggcagg gagaacagag cttccttacg tcgtctccta
     2581 caaaaacaaa ttatgattcg cagcaccctc attctgttgt cgtgacgcga taaaaccgcg
     2641 tctgatttgt gcccatcgtg ttgtcaatga gcgaaatttt gttgcctgta ccagcgactg
     2701 ctccgggaac aacgctccag ttggtatcca agatattatt tttgaattga agttgataag
     2761 tcaacccgac caccgacgtg caactcactg atggcccatt tgtggcgacg ctcattcctt
     2821 gcaacaacaa ctcgccatcc agatgcccgt ccaagttgcg atcaatgccc atcctccggc
     2881 ccgcgccgct cggaaccagc gtgtaagtct gctcgctgcc gatcgcggcc agagcgcgca
     2941 acgcagtgga cgtgtaagtt tctcccggac gatctgaaag aaaaatgctg ccgttcgtgt
     3001 tgaaaaacca accgcgcgcc gcgccgtttt tgaaaccctt gacgatgagt tccacgcgat
     3061 tggtcgggtt gcccgccatg gaaatcatcg tgttgatgag cgcgacggaa tttgaactgt
     3121 tgaccgtgat ttgttttccg acaccggcgg gcgtgtcgag actcggcaat ccaggcgagc
     3181 ggttgatgtc ggtgaggctg cccggaatca ggtccgaacc ggtgaaggag agcaggaatg
     3241 ccacgatgtc ggcagtctgt tgatcgttgg tgagcgacaa tccgtcctgc aaaaaacgga
     3301 cgagcgtgtc cacgccgccg tcatgcaaaa aaccaaaacc agcgcggcta ttggtatggg
     3361 cgaggtccat gcccattttg tcgaagagat tgcgaagtga cggtatcttg aatggcagtt
     3421 cggaggaacg cagcaattcg atgagggcca catgatgcga attgtttgtg ctcaccggca
     3481 atgacaccca atggttcgtg ataaaattca tgtcggtgcc gaggccggtt ggcaatgtat
     3541 ggcagacgat gcaatccggc gtcgtgatga ggcggaaggt gttcatgccg ttcacggcgt
     3601 tgccgttcgg caattgcgcg cccgccggca acgcgccacg gcccagcgcg aaatgcccgg
     3661 gtatcggcaa gttcgttgaa agcgaattat caatctgccg gaacggattg ggcgcaaatc
     3721 gcaccgttgc cagaaaattt ttaaactgct gcatctggtt cgtcgtcaat ccgccgggca
     3781 tcccctgcaa atttgtgaac gtcgggccga acgcctcgat gccttcacgg tcaccgcgcc
     3841 aatggaacgg ctcatgcccg atgatgtcct gcaaggtcaa ggtcgtcatc gggcctttca
     3901 tgggatgaaa attgttggtc gggttcggcg agttgttggc gaagttgaac gtggaatcaa
     3961 tcacatccat cgtcccggtc tggtcgccca agtcccacgc aagccggtcc atccgcgcgt
     4021 ccacatggca ggaaccgcag gacaattgcc cgagtcccga agtcaaatgt gtatcgtaaa
     4081 ggaacggccg tccgctcttg atgacctgcg gcgtggggtc gaacaacggc aacgtcgaaa
     4141 caacggtttg gccaacggta tccaccgtcg aaatgctgtc gtcgaagcga ttgaaaacat
     4201 agagacgatt gtgcctttca tccaatgcca ttcccgtcgg tccctgcccc accgtgatgg
     4261 ccgggttggt tccaacgcga ttgccctgtc catcaatcac gatgagatta tcagagccca
     4321 tgccggtcac gtaaccgcgt gtcccgtcgg aactccaaac gatgccgcgc gggtccccga
     4381 cggacatttc cctttcggcg tctgaaaccg tcggagtttg ataagtgaga tgcggattca
     4441 aatccacaac cgtgttggtc aaagtgacag gatcgacttg cgcgagttcg acccgaacga
     4501 atacgccatc caaaacggat tggaaacgga tttgattgag cgcgtcggta ccgacgacgc
     4561 tgatttttcc ggaagccggg ttcaccgctg tggccatgca aatgttcatc aatccggacg
     4621 cgtaactgat ggaaaaattc gtcgtgttga tcaccgccaa atcatggtcg ggcatgtccc
     4681 atccggggac gcgaccggtg aatgccgcgt ttgtgccgcg gatgaactcc gtccagtcgc
     4741 cattgttgtc gtccatccag cggcccgcgc tgttcttttt cacgatgagg ccggtgcgcg
     4801 gtggcggatt ggtgagcgcg ggattgatgg cgggaacgaa attcgtgccg ctgttcggcg
     4861 gcgggtccag cccatgagat ggcgcgtcgg gaaaggtgat gggacttggc ttcggaaaat
     4921 tgttcaaatc cgaaacgccg gtgctgatta tggtcgaggc gttgccggat tcgaaaatgg
     4981 ccacatacac tttgctgccg tccgggctga cgctcatcgc gcggggacgg tttccatcaa
     5041 tgttaagatt ggtgacgagt tgcgaagaaa tcggattgaa cacttgcacc acgttcggct
     5101 gcccgcagga aacgtaagcg aggttcgacg cgcccgcgaa aacgatgtcc gaaggctggt
     5161 tggtcgtgtt gatggtgttg aagacgcgca tcgtgccgag gtcaatcacg ctgagcgtgc
     5221 tggaaatgta attggcgacc caaagttcgt tggtcgtgcg gaaacagacg ctcaccggat
     5281 tcaggcccac ggcaatgctg gccagcgaaa ccggcgcgcc agaagtcacg tcaaaaattt
     5341 ccacgtggtc gtcaggcaaa ttgcaaacgg cgagtttggt tccatcggga ctgagcgcaa
     5401 ccggatgcac cggtggcgtt tcccaattga agaaattgct tgtggtgaac tgcgcgtccg
     5461 cgtacggaat ggccagcagg caaagcgttg cggcggcaag caacacaagc gctcttttta
     5521 cgcaagcttt ggctccggtc cgcgcaaaga cgcaatgaca cttctgattc atgcgggcag
     5581 tgtcggtgtt atcgggtttc ctattgtgct gccgttttac agcaaacgga cgaatttg
//
LOCUS       DASZMM010000022         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4953640, whole genome shotgun sequence.
ACCESSION   DASZMM010000022 DASZMM010000000
VERSION     DASZMM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4953640"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VG754_00530"
     CDS             <1..563
                     /locus_tag="VG754_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWF17717.1"
                     /translation="FHTLLSKGSDPDSPTMMVFDLDPGPPAGLIECARVACWLHSVLE
                     KLGLQSFAKTSGSKGMQVYVPLNSGASYEETGSFAHALAEGLEQQNPELVVSKMNKSI
                     RGGKVFVDWSQNSRHKTTVCAYSLRAREEPTVSTPLAWDEVERAVVTKKAEGLDFPAD
                     QLLHRVEKLGDLFKLVLKLKQKLPKI"
     gene            579..1415
                     /locus_tag="VG754_00535"
     CDS             579..1415
                     /locus_tag="VG754_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWF17718.1"
                     /translation="MRPIWKGTISFSLINIPIGLYPASRREDISFHMLRQSDLSPINY
                     KRVAENDGREVPWNEIVKGYEYEPGKHVVMKKEDFERVDIEATQTIEIIDFVKLEEID
                     PIFYDTPYYLVPQKGGAKAYALLRDALKKTGRVGIAKVVIKTRQHLAAIKPRNNVLVL
                     ELMHFADELVSPKDLDIPADQNVGAKELDLAIDLIDRMTDKWDPSKYKDDYRNALLDL
                     IHKKVEQGGKPIPGGGGKKKRPTNVVDLASILQESLAQTGKASARKTAKSSAHKSRKK
                     AA"
     gene            1572..3221
                     /gene="ligD"
                     /locus_tag="VG754_00540"
     CDS             1572..3221
                     /gene="ligD"
                     /locus_tag="VG754_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWF17719.1"
                     /translation="MSLKEYKRKRNFSKTSEPAGRGAPRTGKDLHFVIQKHAASHLHY
                     DFRLEMDGVLKSWAVPKGLPMKKGDRRLAVEVEDHPLDYGGFEGTIPPGNYGAGTVML
                     WDRGTYKEASGNPLEALKNGKLHLTLAGKKLKGDWALVRMRPRDNDGGKPQWLIFKSG
                     DDAPPIPVRAEDRSAISRKSMEEIASGNSPQWQSNRQTKPGKSVKKTKTKSATPVFGS
                     DITKLPREKPHFVQPMMCQLTKRLPNGPDWVFEIKFDGVRALGIKTTAKISLISRNEK
                     NLTRKYSEVAEALQSLPCHEAVVDGEVVAVDEQGRSRFQLLQAYEMPGREKAPLLYYA
                     FDLLNLDGHDLKGLPLMRRKELLKSLLASVPDTILFSAGIEADSQRVLKAMQSRGLEG
                     LVAKRRDSKYEPGRRSGAWQKYKWTLEQEFVIGGYTPPKGSRINFGAILVGYYEKNKL
                     MFASKVGTGFDEKLLKHLHEKFQKLIQPDCPFANLPTKHASGSVGLGAAEMSRCTWLE
                     PCLVCEVRFSEWTEEGRLRQPVFLGLREDKKPREVVRESAK"
     gene            3356..3961
                     /gene="lepB"
                     /locus_tag="VG754_00545"
     CDS             3356..3961
                     /gene="lepB"
                     /locus_tag="VG754_00545"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWF17720.1"
                     /translation="MARDEVSIPLVMVSTPEYAAPKVRRSRAGNSFNRYNVIWIVQKL
                     AVLIFMALLSVAVYYFVSQNLVRSVKVVGVSMFPTLHEGSTYLLDRRAFNSRDPEHGD
                     IVVIRDPGDHGLAVKRIIAVSGESIYFKDGNVFVNGKKLDEPYLPSHTPTFTYAKAKA
                     QFITCGQDQYFVLGDNRELSVDSRAYGTVSRQGILGLVKVN"
     gene            4137..>4502
                     /locus_tag="VG754_00550"
     CDS             4137..>4502
                     /locus_tag="VG754_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWF17721.1"
                     /translation="MLSIMSGNLIDVKISTHDRLAELGRRVLSGEQIDYVEATWLFGL
                     ESSSDIFDLMSWANRIREHFKGNKIHLCSIVNAKAGACSENCSFCAQSSFHQTGSPRY
                     GFVDPEPVIEAAEEAHQHGV"
BASE COUNT         1150 a         1128 c         1233 g          991 t
ORIGIN      
        1 aatttcacac tttattgtcg aagggaagcg atccggattc tccgacgatg atggtcttcg
       61 atctagaccc cggcccgccg gcgggactga tcgaatgcgc gcgcgtcgct tgttggcttc
      121 attcagtttt ggaaaaactc ggcctgcaaa gctttgcgaa aacgtcgggc tcgaaaggaa
      181 tgcaggttta tgtgccattg aacagcggcg cgagctatga ggagacgggg tcgttcgcgc
      241 acgcgcttgc ggaaggtttg gaacagcaaa acccggagtt ggtagtttca aagatgaaca
      301 agagtattcg cggcggaaag gtatttgtag attggagtca gaactcgcgg cacaagacga
      361 ctgtttgcgc ctattcgttg cgcgcccgtg aagaacccac tgtttccacg ccgctcgcct
      421 gggacgaagt agaacgcgcg gtcgtcacta agaaagccga aggcctcgat tttccggcgg
      481 accagttgtt gcaccgcgtg gagaagctcg gcgacctttt caagctggtg ctcaagctca
      541 agcaaaagtt gccgaaaatt taaaggaaca gaatcgttat gcgacccatt tggaaaggaa
      601 caatcagctt cagcctcatc aatattccca ttggcttgta tccggcctcg cgccgcgaag
      661 acatcagctt tcacatgctg cgccagtcgg atttaagccc gatcaactac aaacgcgtgg
      721 ctgaaaacga cggccgcgaa gttccttgga atgaaatcgt caagggttac gaatatgagc
      781 cgggcaaaca tgtggtgatg aagaaggagg atttcgaacg cgtggacatc gaagccacgc
      841 agacgataga gataattgat ttcgtgaaac tggaggagat agatcccatt ttttacgaca
      901 ctccgtatta tctcgtgccg cagaagggcg gcgcgaaggc ttatgctttg ttgcgcgatg
      961 cgctgaaaaa aacagggcgt gtgggaatcg ccaaggtggt catcaaaacg cgccagcatc
     1021 ttgccgcaat caagccgcgc aataacgtgc tcgtgttgga attgatgcat tttgccgatg
     1081 aactcgtcag cccaaaggac ttggacatcc ctgccgatca aaatgtcggc gcgaaggaac
     1141 tggatttggc catcgacctc atcgacagga tgacggataa atgggacccg tccaaataca
     1201 aggacgatta tcgcaatgcg ttgctcgatt tgattcacaa gaaggtggag cagggcggca
     1261 agcccattcc gggcggcgga ggcaaaaaga aacggcccac caacgtggtt gatttggctt
     1321 ccattttgca ggagagcctt gcgcaaactg ggaaagcttc cgctcgcaaa accgcaaaat
     1381 cgtccgcgca caaatcgcgc aaaaaagctg cttaaaattt ataaaccgcg gataaacgca
     1441 gataaacgca gatgtggaaa ataagagaga ttttcttggg ccccagttca gaaaaccatg
     1501 agcaacttgc ataacacgaa aatgaatagg tcagaatttc agaatctgca tctatcagcg
     1561 gttaaacctt catgagcctc aaagaataca aacgcaaacg gaattttagt aagaccagcg
     1621 aaccggctgg ccgaggcgca ccgcgcaccg gcaaggattt gcactttgtg atccaaaagc
     1681 acgcggcgag tcatttgcat tacgatttcc ggctggaaat ggatggcgtc ttgaaatcgt
     1741 gggctgttcc caaaggtctt cccatgaaga agggtgaccg gcggctggcc gtcgaagtcg
     1801 aagaccatcc gctggattat ggcggctttg aaggcacgat tccccccggg aattatggcg
     1861 caggcaccgt gatgctttgg gaccgcggaa cctacaagga agcatcaggc aatcctctgg
     1921 aagcgctcaa aaacggcaaa ttgcatctca cactcgccgg gaaaaaattg aaaggcgatt
     1981 gggcgttggt gcggatgcgt ccgcgcgaca acgacggcgg caaaccgcaa tggctgatct
     2041 tcaagagcgg cgacgacgcg ccgcccatcc ccgtgcgcgc tgaagaccga tcggctatca
     2101 gccggaagtc catggaagaa atcgcttccg gcaacagtcc acaatggcaa agcaatcggc
     2161 aaaccaaacc cggcaaatcc gtcaaaaaaa caaaaaccaa gtctgcgacg ccggtcttcg
     2221 gctcagacat caccaaactg ccgcgcgaaa aaccgcattt cgttcagccg atgatgtgcc
     2281 agttgacgaa gcgcttgccg aacgggcctg attgggtttt tgaaatcaaa ttcgacggcg
     2341 ttcgggcatt gggcatcaaa accaccgcca aaatctcttt gatttcacgg aatgagaaaa
     2401 atttgacgcg gaaatattcg gaagtggcgg aagcattgca atcgttgccg tgtcacgagg
     2461 cagtcgttga cggagaagtt gtggcggtgg atgagcaagg ccgttcccgg ttccaacttc
     2521 tccaggcgta tgagatgcct ggccgcgaaa aagcgccgct gctctattac gcatttgatt
     2581 tgttgaattt ggatggccac gatttgaagg gactgccgtt gatgcgcaga aaggaattgc
     2641 tcaagtcgct gctcgcgtca gtgcctgaca cgatcctgtt ttcagcaggc atcgaggcgg
     2701 attcgcagcg ggtcctcaag gcgatgcaat cgcgcggtct ggaaggcttg gtcgccaagc
     2761 ggcgcgattc gaaatatgaa ccgggtcggc gcagtggcgc gtggcagaaa tacaaatgga
     2821 ctttggaaca ggaatttgtg attggcggtt atacgccgcc gaaaggttcg cggatcaatt
     2881 tcggcgcaat ccttgtgggt tattacgaaa agaacaaatt gatgttcgct tccaaagttg
     2941 gcaccggatt tgacgaaaag ttgctcaagc atctgcatga aaaatttcaa aaactcatcc
     3001 agcccgattg cccattcgcc aatctgccga caaaacatgc gagcggtagt gtgggtttgg
     3061 gcgcggctga aatgagccgc tgcacatggt tggaaccatg cctcgtctgc gaggtacgct
     3121 tctccgaatg gactgaagaa ggacgcctgc gacagccagt ttttcttggt ttgcgcgagg
     3181 acaaaaagcc ccgggaagtc gtccgtgaat ccgcgaaata gcctgttttt tccgcacgag
     3241 agggctgact ttaagtgaca cactttccgt agaaaacccg aagctcttcc gtaatcgttc
     3301 aatttgtaac tgcttgaaga tgaggcatgc ttgctttgta acaactgatt gaagtatggc
     3361 acgtgatgaa gtctcaattc cgctggtgat ggtttcaacg cctgaatacg cggccccaaa
     3421 ggttcgccgt tcaagggccg gcaattcgtt caatcgttac aatgtcatct ggatcgttca
     3481 aaagctggcc gttctcattt tcatggcgtt gctgagcgtc gccgtctatt acttcgtcag
     3541 ccaaaatctt gtccgttcgg tcaaagtggt gggcgtgagc atgttcccaa cgctccatga
     3601 aggcagcacg tatctgctcg accgccgggc tttcaacagc cgcgatccgg aacatggcga
     3661 catcgttgtc atccgcgacc ccggcgacca tgggcttgct gtcaaacgca tcattgccgt
     3721 cagtggcgaa tcaatctatt tcaaagacgg gaacgtattt gtgaatggaa agaaactgga
     3781 tgaaccgtac ctgccgtcgc acactcccac attcacttat gcaaaagcca aggcgcaatt
     3841 catcacctgc ggccaggacc aatattttgt gctcggcgat aatcgtgaac tcagtgtgga
     3901 cagccgggcc tacggcaccg tttcgcggca gggcatcttg ggcttggtaa aagtgaattg
     3961 agccggaatt gccattaaag aggcacagaa agcacaaaaa taaaattgtg gatggccacc
     4021 cataaaagcg gagaacacac cgaattcgcc atcctcaata ctcagttgtt gtttttgtgc
     4081 ttcttgggcc ctttcgcggc gattcccagg gcttgcgttg atttccattc cttcctatgc
     4141 tgtcaatcat gagcgggaac ttgattgacg taaaaatttc aacccatgac aggttggctg
     4201 aattgggccg gcgagtgctg tccggcgagc aaatcgatta tgtcgaggcg acatggctgt
     4261 ttggcctcga gagcagttcc gatatttttg atttgatgtc gtgggccaat cgcatccgcg
     4321 aacatttcaa gggcaacaag atccatctct gttccatcgt caatgccaag gcgggcgcgt
     4381 gctcggagaa ttgcagcttt tgcgcgcaat cttccttcca ccaaaccggc tcgccgcgct
     4441 acggctttgt tgatcccgaa ccggtaattg aagccgccga agaagcgcat cagcacggcg
     4501 tc
//
LOCUS       DASZMM010000023         4048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1656121, whole genome shotgun sequence.
ACCESSION   DASZMM010000023 DASZMM010000000
VERSION     DASZMM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656121"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /gene="aroC"
                     /locus_tag="VG754_00555"
     CDS             complement(<1..612)
                     /gene="aroC"
                     /locus_tag="VG754_00555"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWF17722.1"
                     /translation="MANSFGHLFRITTWGESHGGGVGVVVDGCPPKLELTEADIQPDL
                     DRRRPGQSSIVTPRKESDTVQILSGTFRGRTLGTPISLLVKNEDARPEAYSEMATKFR
                     PSHADYTYFAKYGFRNWQGGGRTSARETIGRVAAGAIAKKILRERFGVEVLAYVKQVQ
                     RLVATVSPESVKFRDIEANIVRCPDKAMAEKMIRLIEKMRSAGD"
     gene            705..1166
                     /locus_tag="VG754_00560"
     CDS             705..1166
                     /locus_tag="VG754_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17723.1"
                     /translation="MKSRIRFLAILISVLVLLDSCTFGEKSWKKIENDHFSFSVPESF
                     KKTDRHGIDSFVGVYLGDGIELGFDYGAYSESFRFWPDGTQYETVKVNRKSARIGTMP
                     KGTDREHPYYTQIHIEVTKYTALSMHALCRSEKEVELAKKIFKTIAFKEKK"
     gene            1624..2907
                     /locus_tag="VG754_00565"
     CDS             1624..2907
                     /locus_tag="VG754_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HWF17724.1"
                     /translation="MALTRSNLGRLTEDFFWSAGIEDTFIVDPWPQTGRILDEYELTG
                     HYEKFREDIQLMADLGVRIARYGVPWYRINPKPGKWDWSWADGPLECLLQKGIEPIVD
                     LVHYGVPGWIEGAFANPDFPERMSEYSARLAARFRGRIHAYTPLNEPRITAWYCGKLG
                     WWPPYRRGWRGFVQLMLGICRGIVKTVEALHGVDPEILPVHVDATDLYETNEPDLQEE
                     AKRRQEIVFLALDLISGRIKEEHALLDWLLKMGVAEEELSWFQEHAVDLPMIGINLYP
                     MYSRKVLVSTARGLKIRMPYSSADIVSELADMYWRRYGRPLFISETASMGSIKRRRTW
                     LETSVEAVKKVRERGVQLVGYTWWPMLAIVTWAYRQGTRPPEFYLKQMGLWNVESSSE
                     ARLRRTQTPLVDLFQKLVADGSEAAGKLAYKTKGV"
     gene            2908..>4048
                     /locus_tag="VG754_00570"
     CDS             2908..>4048
                     /locus_tag="VG754_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HWF17725.1"
                     /translation="MFKSFFSAGFECATGYNAGGEWIDQIVATQHDKHVEEDYRRLRE
                     VGIRTVREGIRWPYVDFRGHYDFSSVTPFLKAAWNHGTEVIWDLFHYGYPEDLDIFSD
                     EFRKRFADYCHAVAHYIRSHQEGPYYFTPVNEPSYLSWAGGDKRRFAPHCKGRAGELK
                     IALARAGIEGINAIRAVIPEARMVNVDPICRVALPKEHPEIAAEVERYNEFAIFESWD
                     LLCGKVMPELGGSREHLDIVGMNYYWTNQWELGRDEHPLAPDDERLSPLRDLVRTVWQ
                     RYGGDILITETGHVSDMRASWLNYVTEEALALLEEDVPLRGICLYPILGMPEWHTRHQ
                     WTPMGLWDLENHDSKLHRKIHQPMAEVLRTAQQKLNAAKSIKPVAA"
BASE COUNT          961 a          954 c         1203 g          930 t
ORIGIN      
        1 gtcaccggcg ctgcgcatct tttcgatgag ccggatcatc ttttcggcca tcgccttgtc
       61 gggacaacgg acgatgttcg cttcgatgtc gcggaacttc acgctttcgg gagaaacggt
      121 tgcgaccaac cgctggactt gcttgacgta agcgagcact tcgacgccga aacgttcgcg
      181 caagattttt ttcgctatcg cgcccgcagc aacgcgtccg attgtttcac gcgcgctcgt
      241 gcgtccgccg ccctgccagt tgcgaaaacc gtatttggcg aagtaagtgt aatccgcgtg
      301 cgaagggcga aacttggttg ccatctcaga ataagcttcc ggtcgcgcgt cttcattttt
      361 gaccaacagg ctgatgggcg tgccgagtgt gcgtccgcga aacgtgccgg aaagaatttg
      421 caccgtatcg gattccttgc gcggcgttac gatggaagat tggccgggac gccgccggtc
      481 aaggtcgggc tggatgtcgg cctcggtcag ttcgagcttg ggcggacaac catccacgac
      541 cacaccaact ccgcccccgt gggattcacc ccaggtggtg atgcggaaca gatgaccaaa
      601 cgagttcgcc atggggtaag aatgcgggaa aactggcgtt gggtcaatgt gcttgccagt
      661 tacatcgtta cattgttaaa tggttacgtc gtcacttcaa ggatatgaaa tcacgtattc
      721 gttttcttgc aattttgatc tcagtacttg ttctgctgga ctcgtgcacg tttggcgaaa
      781 aaagttggaa aaaaattgag aatgatcact tctctttctc tgttcctgaa tcttttaaaa
      841 aaactgacag acatgggatc gattcatttg ttggggtata tttgggcgac gggattgagc
      901 ttggctttga ttatggagcg tattcagaaa gctttcgatt ttggccggac ggtacgcaat
      961 atgagaccgt aaaagttaat aggaaatcgg cgagaatagg tactatgccg aagggaacgg
     1021 atagggagca cccgtattac acacaaattc atattgaagt gaccaagtat acggccctga
     1081 gcatgcatgc gctttgtaga tccgaaaagg aagtggagct tgctaaaaaa atctttaaga
     1141 caatcgcttt taaagaaaaa aagtaagaac gctgcagtcc ctcatcggtc atgaagtctt
     1201 tggatgcccc agctttcaaa aaacactcga caactggaag tctggttggc atagtcttgc
     1261 ctgtgtggaa caaaggagca cacaggcacc ccaacggtgt tcgcacatcc gactgttttc
     1321 agccggaagg agattctttt acgaacttgt atgcaatcag cgtcgcaacc ccaattcaat
     1381 accccaccac accaccactc gaattccacg ttcagtcgta agtggttgta aacccgctta
     1441 gaaccgcatg tgttcaccta aatccgccaa aaacggccac cctccccccg ccgtgttatt
     1501 gcggaatgcg gagtgcggga ttgcgaaatg cgccggggat gaaactggat tttctgacgg
     1561 acatggagga gcttatggga agtttgattg gacttaacga aggaggagga tggaaggtga
     1621 accgtggcat tgacgcgaag caacttgggc cgattaaccg aagatttctt ttggtctgcg
     1681 ggcatcgagg acacgttcat cgtggatcct tggccgcaaa ctgggcgcat tcttgatgaa
     1741 tatgaactga ccgggcatta cgaaaaattt cgcgaagaca tccaattgat ggccgacctc
     1801 ggcgtgcgaa tcgcgcgtta cggcgtgcca tggtatcgca tcaatccgaa accgggaaaa
     1861 tgggattgga gctgggcgga cgggccgctc gaatgtctgt tgcagaaagg cattgagccg
     1921 attgtggacc tcgtccatta cggtgtgccg ggatggattg aaggagcgtt cgccaacccg
     1981 gattttcccg agcgtatgag cgaatattcg gcgcgcctgg cggcgcggtt tcgaggcaga
     2041 atccacgctt acactccgtt gaatgaaccg cgcatcacgg cgtggtattg cggcaagctc
     2101 ggttggtggc cgccgtatcg ccggggatgg cgcgggttcg ttcagttgat gctgggaatt
     2161 tgccggggca ttgtgaaaac ggtggaagcc ttgcacggcg ttgacccgga gattttgccg
     2221 gtccatgtgg atgcgaccga tttgtatgaa accaatgagc cggacttgca agaggaggca
     2281 aaacggcggc aggagattgt cttcctggcg ctggacttga tcagcggaag aatcaaggag
     2341 gaacatgcgt tgttggattg gttgctaaag atgggcgtcg cggaagaaga attgagttgg
     2401 tttcaagagc acgcggtgga tttgccgatg attggaatca acctttaccc gatgtattcg
     2461 cgcaaagttt tggtttcgac agcgcgcggg ttgaaaatca ggatgccgta cagttcggcg
     2521 gacattgtca gcgaattggc ggacatgtat tggcggcgtt acggccggcc attgtttatc
     2581 agcgagaccg cttcgatggg ttcgatcaaa cggcgacgga catggctgga aacctccgtg
     2641 gaagctgtaa aaaaagtccg tgaaagaggt gtgcaactgg tcggctacac atggtggccg
     2701 atgctggcga tcgtgacatg ggcataccgg cagggaacgc gtccgccgga attttatttg
     2761 aagcagatgg gcttgtggaa tgttgaatcc agttcggaag cgcgattgcg gcgaacgcaa
     2821 accccgcttg tggatttatt tcaaaagctg gttgcagatg gaagcgaagc agccggaaaa
     2881 ctggcataca aaacaaaggg agtataaatg ttcaagagtt tcttttccgc aggttttgaa
     2941 tgcgccactg gttacaatgc gggtggcgaa tggattgacc agatcgttgc cacgcagcac
     3001 gacaagcacg tcgaggaaga ttaccggcga ttgcgcgagg tcggcatccg caccgtgcgc
     3061 gaaggcattc gctggcctta cgtcgatttc cgcgggcact atgacttcag ttcagtcacg
     3121 ccattcctga aggcggcatg gaaccacggc acggaggtga tctgggattt gttccattac
     3181 ggttacccgg aggacctgga cattttcagc gatgaatttc gaaaacgttt cgccgattat
     3241 tgccatgccg ttgcacacta cattcgaagt catcaggaag gtccatacta tttcacgccg
     3301 gtcaacgagc cgtcgtatct ctcgtgggcg ggcggggaca aaaggcggtt cgcgccgcat
     3361 tgcaagggca gggcgggaga attaaaaatc gccctggccc gcgcgggcat cgagggcatc
     3421 aatgccattc gcgcggtgat tcccgaagcg cgcatggtga acgtggaccc gatttgccgc
     3481 gttgcgttgc cgaaagaaca tcctgaaatt gcggcggagg tcgagcgcta caacgagttc
     3541 gcgatctttg aatcttggga tttattatgc ggaaaggtga tgccggaatt gggcggcagc
     3601 cgtgagcact tggacatcgt tggcatgaat tactactgga cgaaccaatg ggaattggga
     3661 cgcgacgagc atccactcgc gccggatgat gagcggttgt cgccgttgcg tgatttggtg
     3721 cggaccgttt ggcagcggta cggcggcgac atattgatca cggaaaccgg tcacgtcagc
     3781 gacatgcggg catcgtggct gaactatgtg acagaagaag cgctcgcatt gctggaagag
     3841 gacgtgccgt tgcgcgggat ttgtctgtat cccattttgg gaatgcccga atggcatacg
     3901 cggcaccaat ggacgccaat gggtttgtgg gatttggaaa atcatgattc aaaattgcat
     3961 cgcaagatcc atcagccaat ggctgaagtt ttgcgaacgg cgcaacaaaa actcaatgcc
     4021 gcaaagtcaa tcaagcccgt tgcggcgc
//
LOCUS       DASZMM010000024         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4133602, whole genome shotgun sequence.
ACCESSION   DASZMM010000024 DASZMM010000000
VERSION     DASZMM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133602"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VG754_00575"
     CDS             <1..597
                     /locus_tag="VG754_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWF17726.1"
                     /translation="PVREFHLERCKRYVDLAKIWGAKNILLVLGEYIWQREVIPAEAQ
                     WQWAIEACHELGDYADKRKIDIALELEPFRLSLLNNIREMARVVDECAHPRVKANIDI
                     SHLVLSDTGPNELKKLKGRAIHVHLSDCDGKVHGDLPPGRGVVKFMPYLQAIKELNID
                     GVVSIELEYAPDPKKIVEWVEEAYRETAKLMDLAGLRS"
     gene            644..1603
                     /locus_tag="VG754_00580"
     CDS             644..1603
                     /locus_tag="VG754_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWF17727.1"
                     /translation="MRIGFLTDANIARLDWARQNGFGSIQWNRFDTSPVGPRESKWRP
                     FAEQFAAEAKSRDIRISAIGAHYKNPLDPKQMEYARGTFLRAIEVAAHIGVKTVCGFP
                     GAIIELTINPRGDNPVYKPFEDFFPQLLEFWEPLALIAADKGVRIAFEHCPMSVYHLP
                     IMGFNMLSQPAMWERLFNETKCENLGIEWDASHLMCQFIDPVTNIHKFGARIFHVHAK
                     DAFINRQLLEVYGICHPGVSEHRMPGLGQANWAEIIHALLRAGYDSDLNIEGWHDPVY
                     RDHATVPPADIKLAATDRQAGQNLENAGLLIGKRTLEQFVPKE"
     gene            1825..3318
                     /locus_tag="VG754_00585"
     CDS             1825..3318
                     /locus_tag="VG754_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17728.1"
                     /translation="MNIPETQDLHRVSEPAPAVESPFARYVPLLVWVLAITVLLLIPL
                     RIIGYGYLPAGDARRHIAKVFTDKPYAEIIAFNGYYKTDQSPGWEWLLRFLHRDFGWG
                     TEALVTFSVTALILCIFYAPLPWLRRPEAWLAALLAQMVAIPEVMTRLTQARPFLITE
                     GVLIAMLFAWSKPEEKPSWLKIIFTVVGITLSVWMHGTWYLWALPVLAFCLAQQWRSA
                     VWLAACIAAGIIAGALLTGNPIVFLMQQVYQVVAITREHLPQWMLVGEFRPSYGEFDT
                     LALLGGVILWRWLQTGRPPQLFRNPVFCMVAICWALGFKGDRFWADWGVPAALVWLTM
                     QFEVILPSLCDPASLKRLALCGLVAFPLLLHATNDLDRRYTFYQKEIFMDASDPSLKG
                     WLPDSGGLFYHAQMDFFYNTFYKNPQGDWRYILAMEPAMMPGDDLRILRQIQRYNYTP
                     KAYEPWINKMTPADRLVIYSPVEPPLPQLQWHNAIGNIWLGRLPKAH"
BASE COUNT          815 a         1056 c          947 g          833 t
ORIGIN      
        1 ccggtccgcg agtttcattt agaacgttgc aaacgctacg ttgatctcgc caaaatttgg
       61 ggtgcaaaaa atatcctgct cgtgcttggc gaatacatct ggcagcgcga agtcattccc
      121 gccgaggcgc aatggcaatg ggccatcgaa gcctgccacg aactcggcga ttacgccgac
      181 aaacgaaaaa ttgacatcgc gctggaactc gaaccctttc gtctcagcct gctcaacaac
      241 atccgcgaga tggcgcgcgt cgttgatgaa tgcgctcatc cgcgcgtgaa ggcgaacatt
      301 gacatcagcc atctcgtgct ttcggacacc ggcccgaatg aactcaagaa gctcaaaggc
      361 cgcgcaatcc atgtccattt gagcgattgc gatggcaaag tccatggcga tttgccgccc
      421 ggacgcggcg tggtgaaatt catgccttat ctccaagcca tcaaagaatt gaacatcgac
      481 ggcgttgttt ccatcgaact cgaatatgcg cccgatccca aaaaaatcgt cgaatgggtc
      541 gaggaagcct atcgcgaaac cgccaagttg atggacttgg ccggcctgag atcttgattt
      601 caatactcca acaatccaac cctccattgc tttcccctct ctcatgcgaa tcggctttct
      661 aactgatgca aacatcgccc gtctcgattg ggcgcggcag aacggcttcg gctcaatcca
      721 atggaaccgt ttcgatacaa gtcccgtcgg cccgcgcgaa tccaagtggc gtccgttcgc
      781 ggaacaattc gccgccgaag cgaagtcgcg tgacatccgt atctcggcca tcggcgcgca
      841 ttacaaaaat ccgctcgacc cgaaacagat ggaatatgcg cgcgggacat ttctgcgcgc
      901 catcgaagtc gcggcgcaca tcggagtgaa gacggtttgc ggttttcccg gcgcaatcat
      961 cgaactcacc atcaatccgc gcggcgacaa tccggtttac aaaccattcg aagatttttt
     1021 tccgcagttg ctggaatttt gggaaccgct ggcgctcatc gccgcggaca agggcgttcg
     1081 catcgcattc gaacattgtc cgatgagcgt ctatcacctg cccatcatgg gattcaacat
     1141 gctttcgcaa ccggccatgt gggagcggtt gttcaacgaa accaaatgcg aaaatctcgg
     1201 cattgaatgg gacgcaagcc atttaatgtg ccagttcatc gacccggtca cgaacatcca
     1261 caaattcggc gcgcgcattt tccacgttca cgcgaaggac gcattcatca accgccagct
     1321 tctcgaagtt tacggcatct gtcatccggg cgtgtcggaa catcggatgc ccggcttggg
     1381 ccaggccaac tgggcggaaa tcatccacgc cttgttgcgc gccggttatg attccgattt
     1441 gaacatcgaa ggctggcatg acccggttta tcgcgaccac gccacggttc cacccgccga
     1501 catcaaactt gccgcaaccg accgccaagc cggccaaaat cttgagaacg ccggcctgct
     1561 catcggcaag cgcaccttgg aacaattcgt gccgaaagaa tgaagcaaaa ttcatgtaac
     1621 gtagcgcaga gttccactct gctagaacta tagagtacag cgatattgga ttatttcacc
     1681 ccgcgcctcg gattcctcaa ccctgccgac tgcaagtcgg cgatacagca gagtgcaact
     1741 ctgcgctaca agagaagcct tcaaaattat tttcacttcc accattgtgg acaaacccga
     1801 atgctcccga tacgctgacg gcgcgtgaac attccagaaa cacaggactt gcaccgcgtc
     1861 agtgagcccg cgcccgccgt cgaatcgcca ttcgcccgct acgttccatt gctggtctgg
     1921 gtccttgcga tcaccgtgct gttgctgatt cctttgcgca tcatcggtta cgggtacctg
     1981 cctgcaggcg atgctcgtcg gcacatcgca aaagttttca ccgacaaacc gtatgccgaa
     2041 atcatcgcgt tcaatgggta ttacaaaacc gaccaaagcc ccggctggga atggctgctg
     2101 cgatttctgc accgggattt tggttgggga acagaggcgt tggtcacgtt ttctgtcacc
     2161 gcattgatcc tttgtatttt ctacgctccc ctgccctggc tgcgacgtcc cgaagcctgg
     2221 ctcgcagcgt tgctggccca aatggtcgcg attccggaag tgatgacgcg cctcacgcag
     2281 gcgcgccctt ttctgataac agaaggcgtt ctcatcgcga tgctcttcgc gtggtccaaa
     2341 ccggaggaaa aaccttcatg gctgaaaatt attttcactg tcgtcggaat cacgctctcc
     2401 gtctggatgc atgggacatg gtatttgtgg gcgttgccgg tgctggcgtt ttgccttgcg
     2461 caacaatggc gttcagccgt ttggctggca gcctgcatcg cagcgggaat catcgccggc
     2521 gctttgctga ctggaaaccc gatcgtcttc ctgatgcaac aggtgtatca agtcgtggcg
     2581 atcacgcgcg aacacctgcc ccaatggatg ttggtcggcg agttcaggcc gagctacggc
     2641 gaatttgaca cgctggcact tttaggcggc gtgattctct ggcgctggct gcaaacagga
     2701 cgaccgccgc aattatttcg caatccagtt ttctgcatgg ttgccatctg ctgggcgttg
     2761 ggattcaaag gcgaccgttt ctgggcggat tggggcgtgc ccgccgcgct ggtgtggctg
     2821 acgatgcagt tcgaagtaat cctgccttcg ctttgcgatc ctgcctcgct taaacggctg
     2881 gcattatgcg gcctcgtggc atttccgctg ctgctgcacg cgacgaacga cctcgatcgc
     2941 cgttacactt tttatcagaa agaaatcttc atggacgctt ccgatccctc gctcaagggt
     3001 tggttgccgg actccggcgg ccttttttac cacgcgcaga tggatttctt ttacaacacc
     3061 ttttacaaaa atccccaggg cgattggcgc tacattctcg ccatggaacc ggcgatgatg
     3121 cccggggacg atttaaggat tctccgccaa atccagcgtt acaattacac tcccaaggcg
     3181 tacgagccgt ggatcaataa aatgacgcct gccgatcgct tggtgattta cagccccgtt
     3241 gaaccgccgc ttccgcaact ccaatggcac aacgccattg gcaacatctg gcttggtcgc
     3301 ctgccgaaag cacattgatt tttcaagctt gttttgttcg tccccgcagt tgatttgcgt
     3361 ggagactgtt ccgtagcggg gactggaaag tgtgcgggtt caggcgactt tccagtcgcc
     3421 tgaattcttg cattcagaat ccgtaatcaa gaacaagccc aagatgtttc cgcagttgac
     3481 gtatcgactt aaccggaaga tattcaaaaa ataggagaac atattatgag cgcactcgct
     3541 gaaaagaaat gcgcgccttg cgacggcgga actcccgcct tgaagggcgc cgaactggaa
     3601 cggctcgccg cacaattgca cgatgattgg gaagtggtcg atgaacatca c
//
LOCUS       DASZMM010000025         7361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2486184, whole genome shotgun sequence.
ACCESSION   DASZMM010000025 DASZMM010000000
VERSION     DASZMM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486184"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5517)
                     /locus_tag="VG754_00590"
     CDS             complement(<1..5517)
                     /locus_tag="VG754_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWF17729.1"
                     /translation="MSESNLEGIAIIGLTGRFPGAADVDGFWRNLVAGVESICAFSDE
                     ELAASGLDVAALRKTPGYVAARGILEKADWFDAAFFGMNPKEAEVTDPQQRLFLEASW
                     EALENAGYAPSQFQGSIGVYAGMGNNTYYLNNLHSRPDLVQLVGWLTTMMANEKDFLA
                     TRVAYKLNLKGPAININTACSTSLVAVCQACQALMTYQCDIALAGGISVNFPQKRGYY
                     YEEGGITSPDGHCRAFDAQAQGTVSSDGLGIVVLKRLSEALNDGDQIYAVIKGAALNN
                     DGSGKVSFTSPSVDGQTEAIALAQAQSGFASETISYVETHGTGTPLGDPIEIEGLKQA
                     FGLVAKKNFCAVGSVKSNIGHLDTAAGVAGLIKTALALKYKLLPASLHFTKPNPRIDF
                     ANSPFFVNTKLTPWPDGPTPRRAGVSSFGLGGTNAHVVLEEAPAPTPSSPGRDWQLLV
                     FSARTPTALDSATANFVSHLKTNLELNLADAAYTLQVGRNAFNHRRAVVCRDAADAIK
                     TLEASDPKRVFTQYHEVNTRPVVFMFPGQGAQYVDMGAELYRTEPVFAEEVDRCAKLL
                     QPHLGLDLRRAIFPQQGQEKAAEELLIQTRITQPALFVIEYALARLWMSWGVKPGAMI
                     GHSVGEYVAGCLAGVFSLETALELVAQRARLVQMQPGGAMLAVRLPEAEAQSLLQDGL
                     SLAAVNSSNLCVISGPYENIERLERQLDERGIAGRRLQTSHAFHSAMMDPVIVPFTEL
                     LQKAGLNEPTIPYVSNVTAKWITAGDATNPKYWAGHVRQTVRFAEGVGELLKDPNVVL
                     LEVGPGQTLSTLARQHPARRESQTIISSSAPTKDPQQETNAMLNALGKLWLTGVTVDW
                     NGFHQHQKRRRVPLPTYPFERKRFWAEPAARSTQQHVTMPLEKSGSCVENTDADAADS
                     PKSNQPTEIPAASRKEHILATLIKQLEELSGKSAADISLTASFLEMGFDSLFLAQASQ
                     AFFRTFGIKVTFRQLLEQLTTLNDLAEHFDKQLPPEEIPTDLHVSSATPVQGTQAKSA
                     EPLILPLSEAQTEVWLATQWSEDASRAFNQVVSLRLHGPLQMEPLRESLNALVERHDA
                     LRTTFLSDGSGQRIAPGGKLELSAVDLSSQKENERENHLAEIVRTEDAKAFDLVNGPL
                     ARALLIKLAADEHVLLFASHHLVMDGWSISVVCRELGQFYSAFARGEKPSPQPAAQLR
                     DYLQWQTAPENRAASAQAETCWLQQYANPPGPVELPGDRLRPAAPSFRAACESLKLKP
                     ALHQSLKEAAARQGVTLVTYLLASLNALLSRLSGQNDIALGVPAAGQLALGSDEGKLL
                     MGHCVNLLPVRSRCESNASFADYLQSVKRLMLDAYEHQQFTYGNLVQKLKLPRDSSRV
                     PLLTGTFNVMRVPTDIHLEGLQVEFSLAPKGFNLFDISVDVVDSETDLRLDCRFNSDL
                     FDASTFQRWLNHWQTLLEATVNQPECSIGKLPLLDETERRKIIVEWNNTQAAVPPVQC
                     LTQFFEAQVRENPDATALVFKEQRLTYRELNQRANRLAHHLQRHGVGCETLVGVCMER
                     TPEMVIGLLAILKAGGAYVPLDPHYPAERLAFILEDTNAPVLLSQQSLAPTLPPHHTQ
                     IILLDTPIPENEDTNPLNTTQPDHLAYIIYTSGSTGRPKGVAIEHRNALSLIHWAREV
                     FTKDELSGVLASTSICFDLSVFELFVTLSCGGKIVLAENALELPHLAAAQEVTLINTV
                     PSAAAELARQDAIPSSVTTINLAGEPLKTALVQQLYQRPHIRKVYDLYGPSEDTTYST
                     WMLRRADGPETIGRPMTNKQVYLLDALLQPVPVGVTGELYIGGAGVARGYLHRPE"
     gene            complement(5521..>7361)
                     /locus_tag="VG754_00595"
     CDS             complement(5521..>7361)
                     /locus_tag="VG754_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWF17730.1"
                     /translation="RCFHELFEDQTARAPDATAITFHGESLTYDQLNRRANRLAHHLR
                     SLGVGPETLVGLCVERSLEMAIGLLAIFKAGGAYVPLDPNYPSERLAFILEDAKVPVL
                     ITQESFLGNLPKPNGAFAPRVICLDPELSVIGPQSDFDLRPNAEPDNLAYVLYTSGST
                     GKPKGVQIPHRALINFLWSMSRQPGFIASDILLAVTTLSFDIAGLELWLPLMVGGQVV
                     IADNETVRDGKRLAKELEQCGATVLQATPATWRMLLETGWAGNPRLKALCGGEAWGAD
                     LAKALLPKCASLWNMYGPTETTIWSAATQVEQGEGPLIGEPIANTQFYILDEHLQPVP
                     LGVPGELHIGGDGLARGYLNRPELTVEKFVPDPFGKEAGARLYKTGDLVQRHPSGQLE
                     FLGRLDHQVKIRGFRIELGEIEAVLRSHSNVREAVVMARENQGREKRLVAYVVARQPS
                     ASLIRELQSFVAEKLPGYMVPTAFVMLDSLPLTPNGKIDRKALPEPENRPELAANYTA
                     PSTPMEKSIAEIWAEVLHLEKVGVHDNFFDLGGHSLLLARVHARLCETLQINLSIVKL
                     FQHPTINSLAAHLNQTSADNNTLSRVQQRARLQREAMGRREQPVKR"
BASE COUNT         1623 a         2022 c         2113 g         1603 t
ORIGIN      
        1 ttcgggccga tgcaggtatc ctcgtgcgac gcccgcgccg ccgatataaa gttctcctgt
       61 cactcccacc ggcacaggtt gcaacagcgc atccagcaaa taaacctgct tgttcgtcat
      121 cggccggccg atggtttccg gaccgtcggc gcgccggagc atccatgtgg aataggtcgt
      181 gtcctccgac ggcccataca ggtcatagac tttgcgaatg tgcggtcttt gatacaactg
      241 ctggacgagc gcggtcttga gcggttcacc ggcgagattg attgttgtga ccgacgaagg
      301 aatggcatct tgccgcgcca gttccgcagc ggcggaaggg acggtgttga tcaaagtgac
      361 ttcctgcgcg gcagccaggt gagggagttc caacgcattt tccgcaagga cgattttgcc
      421 gccgcaactc aaggtgacaa acaattcgaa caccgacagg tcaaagcaaa tcgaagtcga
      481 ggccagcacg ccggaaagtt cgtccttcgt gaatacttcg cgcgcccaat gaatgagcga
      541 aagcgcattg cgatgttcga tggcgacgcc cttcggacga ccggtcgaac cggaggtgta
      601 aatgatgtac gcgagatggt cgggctgtgt tgtattgaga ggattggtgt cctcattttc
      661 gggaatgggc gtgtccagca aaatgatttg agtatgatgc ggcggcagag tcggggcaag
      721 actctgttga ctgagaagca caggggcgtt cgtgtcctcc aaaataaacg cgagtcgctc
      781 ggcgggataa tgcgggtcaa gcggcacata cgcgccccca gccttcaaaa ttgcgagcag
      841 accgatgacc atttccggcg tgcgttccat gcacacgccg accaatgttt cgcaaccgac
      901 gccgtgccgc tgcaagtgat gcgccagacg gttcgcgcgc tggttaagtt cgcggtaagt
      961 aagccgttgt tccttgaaga ccagcgctgt ggcatcggga ttctcccgga cttgtgcctc
     1021 gaaaaattgc gtcaggcatt gcacaggcgg aacagcggcc tgcgtgttgt tccattcgac
     1081 gataattttg cggcgttcag tttcgtcgag caacggcaat tttccgatgg aacattccgg
     1141 ctggttcact gtggcctcaa gcaatgtttg ccaatgattc agccaacgct gaaacgtgga
     1201 ggcatcgaac aggtcgctgt tgaaacggca gtcgaggcgc aagtccgttt ccgaatccac
     1261 cacatccacg ctgatgtcga aaaggttgaa acctttgggc gcgagcgaga attcgacctg
     1321 aagtccctcc agatggatgt ccgtgggaac ccgcatgaca ttgaacgtgc cggtcaacaa
     1381 cggcacccgg ctggaatcac gcggaagttt caatttctga acgagattgc cgtaagtgaa
     1441 ttgctggtgt tcgtatgcgt cgagcatgag gcgtttgacc gattgcaaat aatccgcaaa
     1501 ggacgcgttg ctttcacagc gactgcgcac cggcaaaagg ttcacgcaat gtcccatcaa
     1561 caattttcct tcgtcgctgc ccaaggcgag ttgaccggca gccggaactc ccaacgcaat
     1621 gtcattctgc ccactcaagc gggagagcaa tgcgttcagg ctcgccagca aataagtgac
     1681 aagcgtgact ccctgtcgcg cggcggcttc tttcagcgat tgatgcagcg cgggcttgag
     1741 cttcagcgat tcgcacgcgg cgcggaagct tggagcggcg ggacgcaaac ggtcgccggg
     1801 cagttcaacc ggcccgggtg gattggcata ttgctggagc cagcaagttt ccgcttgcgc
     1861 agaagcggcg cgattttcag gggcggtttg ccattgaaga taatcgcgca actgcgcggc
     1921 gggttgcggc gatggttttt ctccgcgcgc gaaagcagaa taaaattgac cgagttcgcg
     1981 gcaaacaacg ctgatggacc agccatccat cacgagatga tgcgatgcaa aaagcaggac
     2041 gtgttcatcc gccgcaagtt taatgagcag cgcgcgagca agagggccgt tgaccagatc
     2101 gaatgccttt gcatcttctg tccgcacgat ttcggccaga tgattttccc gctcgttctc
     2161 cttttgggaa gaaagatcaa ctgccgacaa ttccagcttg cctccgggcg cgatgcgttg
     2221 acccgaacca tcggacagaa aggtggtgcg cagggcatca tgacgttcga ccagcgcatt
     2281 caacgattca cgcaacggtt ccatttgcaa tggaccgtgc aagcgcaacg aaacgacttg
     2341 gttgaacgcg cgcgaggcat cttcactcca ctgcgtggcg agccaaactt cagtctgcgc
     2401 ttcgctcaaa ggcaaaatga gcggctcggc ggatttagct tgggttccct gaaccggagt
     2461 cgcagaagac acgtgaaggt cggttggaat ttcttctgga ggcaactgtt tgtcgaaatg
     2521 ctccgccaaa tcgttgagag tagtgagttg ttccagcaat tggcggaagg tcaccttgat
     2581 gccaaatgtg cggaaaaatg cctggcttgc ctgcgcgagg aacaacgaat caaaacccat
     2641 ttcgagaaaa cttgccgtaa gcgagatgtc cgccgcgctt ttgccggaca attcctcaag
     2701 ctgtttgatg agggtcgcaa gaatatgttc cttgcgggac gccgcaggaa tttcagttgg
     2761 ctgattggat ttcggcgaat cagcagcgtc ggcatctgtg ttttctacac aggaaccgct
     2821 cttttccagc ggcatagtga cgtgctgttg ggtggatcga gcggccggct cggcccaaaa
     2881 acgttttcgt tcgaatggat aagtgggcag cggaacgcgc cggcgttttt gatgttgatg
     2941 gaaaccgttc caatctactg tcacgccagt caaccagagc tttccaagcg cgttaagcat
     3001 cgcgttggtt tcctgttgtg ggtcctttgt tggggcagaa gaagaaatga ttgtttgcga
     3061 ttcgcgccgg gccggatgct gccgcgccag cgtgctcaat gtctggccgg ggccgacttc
     3121 gagcaaaact acatttggat ctttcaacaa ttcgccgaca ccttccgcga accgcacggt
     3181 ttgtcgcaca tgccccgccc aatattttgg attggtggcg tccccggctg tgatccattt
     3241 ggccgtcacg ttcgagacat acgggatggt cggttcattc agccctgctt tctgcagcaa
     3301 ttcggtgaaa ggaacgatga cggggtccat cattgccgaa tgaaacgcgt gcgaagtctg
     3361 aagtcttcgt cccgcaatgc cgcgctcgtc caattgccgt tccagacgct cgatgttctc
     3421 gtaggggccg gaaatgacgc acaggttgga cgagttgacg gcggccaggg aaagtccatc
     3481 ctgcaacaac gattgagctt ccgcttccgg caaacgcacc gcgagcattg cgccgccggg
     3541 ctgcatctgg acgagacgcg cgcgttgcgc gaccagttcc aaagctgttt ccaacgaaaa
     3601 gactcccgcg agacaaccag cgacatattc gccgacgctg tggccgatca tcgcgccggg
     3661 cttcacgccc cacgacatcc agagccgcgc cagggcgtat tcgatgacaa acaaagcggg
     3721 ttgcgtaatt cgcgtttgaa tcagcaattc ttccgcggct ttttcctggc cttgttgggg
     3781 gaaaattgcc cgtcgcaaat ccaatcccaa atgcggctgc aacaacttgg cgcaacggtc
     3841 cacttcctcg gcaaaaaccg gttcggtgcg atacaactcc gcgcccatgt cgacatattg
     3901 cgcgccctgg ccgggaaaca tgaagacgac cggccgcgta ttcacttcat gatactgtgt
     3961 aaaaacgcgt ttcggatcag aggcttcaag cgtcttgatg gcgtctgcgg cgtcacggca
     4021 aacgacagcg cgtcggtgat tgaaagcatt gcggccaact tgaagcgtgt aagccgcatc
     4081 tgccagattc aattccagat tggttttcaa atggctgacg aaattggccg tggccgaatc
     4141 caatgcggtt ggcgttcggg cggagaaaac aagcagttgc caatcgcggc ccggactgga
     4201 aggcgtcggc gctggcgctt cctcgagcac gacatgcgcg tttgtgccgc ccaacccaaa
     4261 cgaactcacg ccggcgcgac gtggagttgg gccatccggc cagggcgtca actttgtgtt
     4321 cacaaaaaac ggactgttcg cgaaatcgat gcgcgggttg ggcttggtga aatgaaggct
     4381 ggctggcagc aatttatact tgagcgcaag cgcggtcttg atgagacccg caacacccgc
     4441 ggctgtgtcg agatggccga tgttcgattt gacggacccg acggcgcaaa aatttttctt
     4501 cgccacgagg ccaaacgcct gtttcaagcc ttcgatctca atcggatcgc ccaacggtgt
     4561 tccggtgccg tgcgtttcga cgtaagaaat ggtttccgaa gcaaaaccgg attgcgcctg
     4621 cgcaagcgcg atggcttcgg tctggccgtc cacgctcggc gaagtaaagc tgactttgcc
     4681 agagccgtca ttgttcaagg ccgcgccttt gatgactgcg taaatttggt cgccatcgtt
     4741 caacgcttcg cttaaacgct tgagcaccac aatgccgagg ccgtcactgg aaacggtgcc
     4801 ttgcgcctgc gcgtcgaaag cccggcaatg accgtccggc gaggtgatgc cgccttcctc
     4861 gtaataataa ccgcgctttt ggggaaagtt gaccgagatg ccgccggcga gcgcaatgtc
     4921 gcattgataa gtcatcaacg cctgacaggc ctgacaaacc gcgacaagtg aagtggagca
     4981 ggcggtgttg atgttgatgg ccggcccttt gagattcaat ttgtaggcaa cgcgcgtggc
     5041 gagaaaatct ttctcattgg ccatcatcgt cgtcagccag ccaaccaatt gcaccaaatc
     5101 cggccgcgaa tggagattgt tcagataata ggtgttgttt cccatccccg cataaacgcc
     5161 gatggaacct tgaaattggc tgggagcata acctgcgttt tccagcgctt cccatgatgc
     5221 ttccaaaaaa agacgctgct gcggatcggt cacttcggct tccttgggat tcatgccgaa
     5281 gaacgcggcg tcgaaccaat ccgctttttc caaaatgccc cgcgcagcaa cgtaaccggg
     5341 agtttttcgc agcgcggcaa catccaaacc ggaagcggca agctcttcat cactgaacgc
     5401 gcaaatggat tccacaccgg caaccaagtt gcgccagaat ccatccacat cggccgcgcc
     5461 gggaaacctg ccagtcaggc cgatgatggc gatgccctcc agatttgatt cgctcataag
     5521 tcagcgtttg acgggttgct cgcgccggcc catcgcttcg cgttgcaatc gggcgcgttg
     5581 ctgaacacgg ctcagcgtat tgttgtctgc cgaagtctgg tttagatgcg cggccagcga
     5641 gttgatggtt ggatgctgaa aaagtttaac gatggaaaga ttgatttgga gcgtttcgca
     5701 aagccgcgcg tgaacgcgcg ccaggagcag cgagtgtccg ccgaggtcaa aaaaattgtc
     5761 atgcactccg actttttcca agtgcaagac ctctgcccaa atttcggcga ttgatttttc
     5821 catcggggtg cttggcgcgg tgtaattcgc tgcgagttcg ggccggtttt ccggttctgg
     5881 caaagctttg cggtcaatct tcccattcgg tgtgagcggc aaactgtcga gcatcacgaa
     5941 agctgtcgga accatgtagc cgggaagctt ttcggccacg aacgattgaa gttcgcgtat
     6001 gagcgatgcg gatggttgcc tcgcgacaac gtatgccacg agacgttttt cgcggccttg
     6061 attttcccgc gccatgacaa cggcttcgcg cacgttcgaa tggctgcgca aaaccgcttc
     6121 aatctctccc aactcaatgc gaaagccgcg aatcttcacc tgatgatcca gccgtcccaa
     6181 aaattccaat tgtcctgaag gatgtcgttg gaccaaatcg ccagttttgt atagccgcgc
     6241 gccggcctcc tttccaaacg ggtcgggcac aaacttttca accgttaatt ctggacgatt
     6301 caggtagccc cgcgccaaac cgtcgccgcc gatgtgcaac tcgcccggca cgcccaacgg
     6361 aaccggttgg agatgctcgt ccagaatgta aaactgcgta tttgcgatcg gttcgccaat
     6421 caacggcccc tcaccctgtt caacctgtgt tgccgccgac caaatggtcg tttccgtcgg
     6481 cccatacata ttccacagag aagcgcattt cggcagcaat gcctttgcca aatccgcgcc
     6541 ccaagcttcg ccaccgcaca aggctttcag ccgtggattg cctgcccaac cggtttccaa
     6601 gagcattcgc caagtggcgg gcgtcgcttg caagacggtt gcaccgcatt gctccaattc
     6661 tttcgccagg cgtttgccgt cgcggacggt ttcattatcg gcaatgacga cttgtccgcc
     6721 gaccatcaat ggaagccaca attccaatcc tgcgatgtcg aaggaaagcg tcgtgacagc
     6781 cagaagtatg tccgaggcga tgaagccggg ttgccggctc atgctccaaa gaaaattaat
     6841 cagcgcgcgg tgcgggattt ggacgccttt gggcttgccg gttgagccgg aggtgtagag
     6901 gacataagcg agattgtccg gctcggcatt tggacggagg tcaaaatcgc tttgcggccc
     6961 gatgacgctg agttcagggt cgagacaaat cacgcgcggg gcgaacgctc cattcggttt
     7021 tggcagattt ccgagaaagg attcctgcgt gataagcaca gggactttgg cgtcttcgag
     7081 aatgaatgcc aatcgctcgg acggataatt cgggtccaat ggcacataag cgccgccagc
     7141 tttaaaaatt gcgagcaggc cgatggccat ttccagagag cgttcgacgc aaagcccgac
     7201 gagtgtttca ggacccacgc ccaatgagcg caaatgatgc gcaagccggt tggcgcgcct
     7261 gttcaattga tcgtaggtaa ggctttcgcc gtgaaatgtg attgcggtcg catcgggcgc
     7321 gcgagcggtt tggtcttcaa acaattcgtg aaagcaacgg t
//
LOCUS       DASZMM010000026         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4404382, whole genome shotgun sequence.
ACCESSION   DASZMM010000026 DASZMM010000000
VERSION     DASZMM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4404382"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VG754_00600"
     CDS             <1..515
                     /locus_tag="VG754_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17731.1"
                     /translation="RKSHFMGRWMRHSGWYPDYRQPQFFNRRRMRYRNDVVHEGFTLS
                     GRLGYLREHVLQYPWPTIEAGMAKLQRYSTLMAHRYAGAQKQATMGRLLGHPVGTFLK
                     TYLLQQGFRDGRHGFILAAMYSYYTFLKYAKLWELQHQQAATVPVATAATHERPAVVG
                     SELCLEKGRS"
     gene            687..1433
                     /locus_tag="VG754_00605"
     CDS             687..1433
                     /locus_tag="VG754_00605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWF17732.1"
                     /translation="MSSAPAHYTSLKPFRDFFASGMPCLTYHKIAPRPRGVRIKGLYL
                     NPRLFQKQMGELAKAGYTTPPYGHVPIGPNGGHTLSLSFDDGFVNAFRMALEILAQYK
                     FRAIQFLVADRIGQFNEWEVQLGDVREPLMDTSQIREWLAAGHQIGAHTLTHPFLTRV
                     SIREAREEIFSSKKKLEDLFGLPIRHFCYPYGDRNQAIRDMVLEAGYTTACTTDFGVN
                     TADTHPLELRRIMARHRSISIKAIKAQLAG"
     gene            complement(1481..1696)
                     /locus_tag="VG754_00610"
     CDS             complement(1481..1696)
                     /locus_tag="VG754_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17733.1"
                     /translation="MKTKIVSKLTGDGSALGLERTVLDHHLAGNTRSLQVPHDYDAVE
                     GGTEFHPSALSKGIRQIEPWRRGGINE"
     gene            2086..2277
                     /locus_tag="VG754_00615"
     CDS             2086..2277
                     /locus_tag="VG754_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17734.1"
                     /translation="MNAIRVSGTRTKAGPVLMVLAAGVAIIALTVYAILSLTAPVRPE
                     SSGEVAPPQDNTPFVGKKW"
     gene            complement(2302..>3162)
                     /locus_tag="VG754_00620"
     CDS             complement(2302..>3162)
                     /locus_tag="VG754_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17735.1"
                     /translation="IRYSICTLVTNMAEYSEMVRSFMQAGFDPSFCEFIYLDNSKENR
                     FDGFRGYNLFLDRAHGEFIILCHQDILLFDDRLEDLERCMRELTERDPNWALFGNAGG
                     GLKLGTRSLCITDPDGGHNSQDFPAEVGTLDENFIVVKRAANLSLSRDLSGFHLYGTD
                     LCLVARLRGYKIWVMDFNLFHKSRGNCDGHFKELKKTLIRKYCDAFKGRYVETTCTRF
                     YISGYRWQSVFFQTGIVRSTIKRWNRSKKKWGNTFRKRRATILRVWNTLRGSPAAQAP
                     NTSGPEIRTP"
BASE COUNT          706 a          899 c          852 g          705 t
ORIGIN      
        1 ctcgcaagag ccatttcatg ggccgctgga tgcgccattc cggctggtat cccgactacc
       61 gccaaccgca atttttcaac cgccgccgga tgcgttaccg caacgatgtc gtgcatgaag
      121 gattcactct cagcggacgg ctcggctatc ttcgtgaaca tgtcttgcaa tatccctggc
      181 cgaccatcga ggcgggcatg gcgaaactgc agcgttactc gacgttgatg gcgcatcgct
      241 acgcgggagc gcaaaagcaa gcgacgatgg gccgattgct cggccatccg gtggggacat
      301 ttttaaagac ctatctgctc caacagggat ttcgagacgg acggcatggg ttcatcctcg
      361 cggccatgta cagctattac accttcctga agtacgcaaa gttatgggaa ttgcaacatc
      421 agcaggctgc gacggttccg gttgcgacgg cagcaactca cgaacgtccg gcggttgtgg
      481 gctccgagct ttgcctggag aagggcagaa gttgaccgcc cgttgcgcgt tccggaggcc
      541 tgtcagcgga tgaacagcac gccaaacttt cggcgtagcg ggcgaattcg cgcagcccgg
      601 catttcattc tgccagatat gccgggagga accggggagc tttatttcaa attgtctggc
      661 gcagtcagcc gtgttacaac ttcaccgtgt catccgctcc ggcgcactat acttcactga
      721 agccgttccg cgattttttc gcgagcggca tgccctgtct tacgtaccac aagatcgccc
      781 cgcgcccgcg gggcgtgcgc atcaagggtc tttacctgaa cccgcgcctg ttccaaaagc
      841 agatgggcga acttgccaag gcgggctaca ccacgccgcc ttacggccat gttcccatcg
      901 gcccgaacgg cgggcataca ctttcgttga gcttcgacga cggtttcgtg aatgcgttcc
      961 ggatggcgct tgaaatcctc gctcaatata aatttcgcgc gatccaattt ctcgtcgccg
     1021 accgcatcgg ccagttcaac gaatgggagg tgcagcttgg cgacgtgcgc gaaccgctca
     1081 tggacacttc gcagatccgc gaatggctcg cggcgggcca tcagatcggc gcgcacacat
     1141 tgacgcatcc gtttttgacg cgggtgagca tccgcgaagc gcgcgaggaa attttctcca
     1201 gcaagaaaaa actcgaagac cttttcggac tgcccatccg ccatttttgt tatccttacg
     1261 gcgaccgcaa ccaggccatc cgcgacatgg tgctcgaagc cggttacacg accgcctgca
     1321 cgaccgactt cggtgtgaac accgccgaca cgcatccgct cgaattgcgc cgcatcatgg
     1381 cgcgccatcg cagcatcagc atcaaggcca tcaaggcgca attggcgggg tgagattttg
     1441 cacaaaaatt ttttcaaaaa caaaatttta aaagccgcgc ttattcgttg attccgccgc
     1501 ggcgccacgg ttcgatctgg cgaatgcctt tgctcaaggc gctgggatga aattccgtgc
     1561 cgccttccac agcatcataa tcatgaggca cttggagcga ccgtgtgttt ccggcaagat
     1621 gatgatccaa gacggtccgc tccaggccca atgccgatcc atctccagtc aatttgctca
     1681 ctatttttgt tttcatcccc ttcacgctat gccaaccgcc tcttcgcgaa catcagggca
     1741 aggctgatat tgccatagga gtttgtctta tgtttggagg gcgcatgacc ggctgcggtg
     1801 gctgccccgc atccgcgtgc cgctgatggg cgcggttttg agacggaacg aggacggctt
     1861 tttgttccct ttgttttccg caaagggcat cgccagaatg gcgatgatgg ctatcaatgg
     1921 cattttgcaa gatttcactt ttcggtgtcg gccgggaaca aaagagagat atcaaacatc
     1981 tttgtattga ttgtagcgca atcaatatga taaagaacag caaaccctaa ctagacgtga
     2041 aactgtcctt aatcatccct ctggatgatt ttggccgagg gtgatatgaa cgcaattaga
     2101 gtatctggaa cgaggacgaa ggccgggccc gtgttgatgg tcctggctgc gggtgtcgca
     2161 atcatcgcat tgaccgtgta tgcgattctt tcgctgacag cgccagtcag gcctgaaagc
     2221 agcggagaag tggccccacc gcaggacaac actccctttg tcggtaaaaa gtggtgaact
     2281 gcgcgccggg attttccgcg ctcaaggagt gcggatttcc ggaccgctcg tgtttggcgc
     2341 ctgcgcggcc ggggagcctc tcaaggtgtt ccacacccga aggattgtcg cgcggcgctt
     2401 gcgaaaagtg ttcccccatt ttttcttgga ccggttccag cgcttgatcg tcgagcggac
     2461 gatgcccgtt tgaaagaaaa cgctctgcca tcggtacccc gatatataaa agcgcgtgca
     2521 ggtcgtctcg acgtagcgcc ccttgaaggc gtcgcaatat tttcttatca acgttttttt
     2581 cagttctttg aaatgcccgt cgcaattgcc gcggcttttg tggaacaggt tgaagtccat
     2641 cacccatatt ttataacccc gcaacctcgc cacgaggcaa aggtcggtcc cataaagatg
     2701 aaaacccgag aggtcgcggg aaagtgaaag gttagcggcc cttttcacca ctatgaaatt
     2761 ttcatccaat gtgcccactt cagccgggaa atcctgggag ttatgcccgc catccgggtc
     2821 ggtgatgcaa agcgaacggg tgccaagctt caatccaccc ccggcattcc caaaaagcgc
     2881 ccaattcggg tcgcgctcgg tcaactcgcg catgcaacgc tccaagtcct caaggcggtc
     2941 gtcaaaaagc aagatgtcct gatggcaaag aatgatgaac tcgccgtgcg cgcggtccaa
     3001 aaaaagattg tacccgcgaa aaccatcgaa ccgattttcc ttcgaattat cgagataaat
     3061 gaattcgcaa aacgaagggt cgaacccggc ttgcatgaac gaacgcacca tctccgaata
     3121 ttcggccatg ttcgtcacca acgtgcagat cgaatagcgg at
//
LOCUS       DASZMM010000027         5192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_832341, whole genome shotgun sequence.
ACCESSION   DASZMM010000027 DASZMM010000000
VERSION     DASZMM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832341"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..494)
                     /locus_tag="VG754_00625"
     CDS             complement(63..494)
                     /locus_tag="VG754_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17736.1"
                     /translation="MKRILFSTLAAMFLFATLALPVRAGDLESMTGKWSCIRTNENGL
                     VCTQTLEIKKDKFTFRVTDSDNKLMLHARGDVKLGKSSELTIATFENIEAGRSDDEWN
                     EVNDDRHCVYFLDGDSLTLGLNFDKDREKGPRIEVYTRAQK"
     gene            complement(491..1504)
                     /locus_tag="VG754_00630"
     CDS             complement(491..1504)
                     /locus_tag="VG754_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17737.1"
                     /translation="MTNLLIGLMGVLMATNQLAAATNFVSQTTGINVSVPNTNDPVEM
                     EYEKLLAADNAAQGEVDGWIRENQKFADKGGGVPAGELNARIRNRFDSVTKGYEDFIA
                     KHPDHARVRLAYGSFLNDINREDDAVPQFEKARELDPKNPAPWNQLANYYGHKGPVKK
                     AFEYYAKAIELDPKEPVYYQNMGTTVYLFRKDAKEFYDINEQQVFDKALALYNQAMKL
                     DPQNFELAQDVAQTYYGIRPPRTEEALKAWTNCLNLAQAPLEREGVYLHLARWKLNDG
                     RFAEAHQHLSMVTNPMYNDLKARLLRNLKKRENPDSTNELAPKTDINQSTQSEAGKPA
                     PKP"
     gene            1790..2392
                     /locus_tag="VG754_00635"
     CDS             1790..2392
                     /locus_tag="VG754_00635"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWF17738.1"
                     /translation="MELDLEGKYADRAYPILASLVTPRPIAWVTTLGPDGVVNAAPFS
                     FFNLLGADPPILGFCPGDRDDGTPKDTARNIRLTHEFVVNLVDEGLAEAMNRTAETLA
                     YGVSELESAGLTAAPSSVVKPPRIAEAPASMECKEWGTLQIGGNRLIVGLIKRVWVRD
                     ELFDPKTLRVRSESFHVIGRMASPNWYCRTRDRFEMVRPA"
     gene            2441..3322
                     /locus_tag="VG754_00640"
     CDS             2441..3322
                     /locus_tag="VG754_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWF17739.1"
                     /translation="MSTKQLLSLGLCLMASLAPNFSSRAENKIPDECKTGGFFIGCQA
                     YTFNHYSVFEAIEKTAAAGGKVIEFYPGQKLSPEEPNVKWDHNASPETVQKVKDHLAK
                     YGIKAVNYGVVGIPKEEEGARKIFEFAKTMSLHGVTTESVDAIDTIEKLVKEYDIKVG
                     FHDHPKRHNDPNYRMWDPNYILSVVKDRDPRIGSCADTGHWVRSGLKPVDCLRILKGR
                     IISSHLKDLNEMSHGAHDVPYGEGVSDIPGILEELKAQGFDGNISIEYETDWTSSVPE
                     VAQCIGFVRGYGTVKNW"
     gene            3347..3772
                     /locus_tag="VG754_00645"
     CDS             3347..3772
                     /locus_tag="VG754_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF17740.1"
                     /translation="MKTEIQPYLFFSGRCEEALEFYKTALGATVDFMMRYKESPEPPP
                     PGRVPPGFENKIMHATFRIGTNILMASDGCEEGGKFEGFSLAISVPTEAEAKRAFTAL
                     SAGGKVDLPLTKTFWSPCFGMLTDKFGIGWMVSVMGQQP"
     gene            4007..>5192
                     /locus_tag="VG754_00650"
     CDS             4007..>5192
                     /locus_tag="VG754_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWF17741.1"
                     /translation="MGFASLREFFAQAASVTPDQFEEWNKAWRVAVDNGSQESLLTFI
                     SREKGIAEDVFLQQLAKALNWPFLEPAKRDVVPEARQKIPTKVAFQYSVLPTQFENGE
                     LQVVISNPFDAAMMNAVRFNAGCPVTFALAAKIEIEKALKKYYGVGAETLDEMSKDEP
                     IELLVGEDKEITEGDQEASVIKFVNQIIWEAFKDRATDIHFEPAEDELRIRYRIDGIL
                     HQTPMPPQLKRYQAALISRIKVMSGMNIAEKRLPQDGRINVRIKGEEIDIRVSTVPTV
                     YGESVSLRLLTRGKIFLSLEKLGFSQLEENAIREIILKPHGIFLVTGPTGSGKSTSLY
                     AFLSSINSVHKRIITIEEPVEYELKGINQIAVRSDIGLTFAMGLRHILRQDPNVIMVG
                     EIR"
BASE COUNT         1176 a         1368 c         1456 g         1192 t
ORIGIN      
        1 ggccggggtg agggcgagcg taatcactga cattctcatg atcggcttaa tattgcatct
       61 actcactttt gcgcgcgcgt ataaacctcg atgcgcgggc ctttttcgcg gtccttgtcg
      121 aagttcaaac cgagcgtgag cgaatcgccg tccaaaaagt aaacgcaatg acgatcatca
      181 ttcacttcgt tccactcgtc atcagaacgt ccagcttcga tgttctcaaa agtcgcgatg
      241 gtcagctcgg agcttttgcc gagcttcacg tcgcccctgg catgcagcat cagcttgtta
      301 tcggaatctg tgacgcggaa ggtgaacttg tcctttttga tttccagggt ctgtgtgcag
      361 accagcccgt tttcattggt gcggatgcac gaccatttgc cggtcatgct ttccagatca
      421 ccagcacgga cgggcaatgc cagagttgcg aacaggaaca ttgctgcaag agtggagaag
      481 aggattcttt tcatggtttt ggagcgggtt tgccagcttc ggattgtgtg gattggttga
      541 tgtcggtttt cggcgcgagt tcattggtgc tgtcgggatt ttcgcgcttc ttgagattgc
      601 ggagcaaccg ggccttgaga tcgttgtaca tcggatttgt gaccatggag aggtgttgat
      661 gggcttctgc gaaacggccg tcattgagtt tccagcgcgc gagatgaagg taaacaccct
      721 cgcgttcgag cggcgcttgc gcgagattca ggcaattggt ccacgccttc aacgcttctt
      781 ccgtgcgcgg cggacgaatg ccgtaatagg tctgcgcgac gtcctgcgcg agttcaaaat
      841 tctgcggatc aagcttcatc gcctggttat aaagcgcgag cgccttgtca aagacctgct
      901 gctcgttgat gtcgtaaaat tccttcgcgt ccttgcggaa cagataaacc gtcgtgccca
      961 tgttttggta atagacgggt tccttcgggt ccaactcaat cgccttcgcg taatattcga
     1021 acgctttttt caccgggcct ttgtgtccgt aataattggc cagttggttc cacggcgcgg
     1081 gattttttgg atcgagttcg cgcgctttct cgaattgcgg cacggcatcg tcttcacgat
     1141 tgatgtcgtt gagaaaactt ccgtaagcga gcctcacccg cgcgtgatcg ggatgcttcg
     1201 cgatgaaatc ttcgtagccc ttggtgaccg agtcgaagcg attgcggatg cgcgcgttca
     1261 actcgcctgc gggaacaccg ccgcccttgt ccgcgaactt ctggttttcg cggatccaac
     1321 cgtccacttc gccctgcgcg gcattgtcag ccgcgagcaa tttttcatat tccatctcga
     1381 ccgggtcgtt ggtgttggga acagagacat tgatgccagt ggtttgcgaa acgaaatttg
     1441 tggcggccgc cagttggttg gtggccatca agacgcccat cagcccgatg agaaggttag
     1501 tcacaatggc aacatagcat cgggtaatgc tgagtcgagc gtggaagttg aggcgtgcat
     1561 gttgacactg ttcttgattg aggttcctga acgcgaaagt caggcgactg gaaagtcgcc
     1621 tgaacccgca gacaagaatg tctgcgctac gtggcgcagg ctttccagcc tgcgggttgc
     1681 ggggactttc cagtccccgc tccagaacag tgtcgaagat gcgcgcgaag ttgaatgggg
     1741 ccggaaaatt ttaatgcgat tcggaatggg atgtgttatt ccattggcga tggaactcga
     1801 tctggaagga aaatatgcgg atcgcgccta tcccatcctg gcttcgctgg tcacgccccg
     1861 tccaattgcg tgggtgacga ctcttgggcc cgatggcgtg gtgaacgcgg cgccgttcag
     1921 ttttttcaat ttgttgggcg ccgatccgcc gattcttgga ttttgcccgg gcgaccgcga
     1981 cgacggcacg cccaaggaca cagcgcggaa catccggttg acgcacgaat ttgtcgttaa
     2041 tctcgtggat gaagggcttg cggaggcgat gaatcgcacg gcggagacct tggcttatgg
     2101 cgtgagcgaa ttggagagcg ccgggctgac tgccgcgccg agttccgtgg tgaaaccgcc
     2161 gcgcatcgcg gaagcgccgg cgagcatgga atgcaaggaa tggggaacgc tgcagatcgg
     2221 cggaaaccgg ctcatcgtcg gattgatcaa acgcgtctgg gtccgcgatg aattgtttga
     2281 tcccaaaacg ctgcgggtcc gtagcgagtc attccacgtc atcggccgca tggccagccc
     2341 gaattggtat tgccgaacgc gcgatcgttt tgaaatggtc cggcctgcat gatactctta
     2401 ccgcaacaca aattccattt atcgcaacac aaattccaat atgtcaacca aacaacttct
     2461 atccttggga ctctgcctca tggcatcact cgcaccaaac ttttcaagcc gcgccgaaaa
     2521 taaaatcccg gacgaatgca agaccggcgg ttttttcatc ggctgccagg catacacgtt
     2581 caaccattat tcggtgtttg aggccatcga gaaaaccgct gcagcaggcg gcaaggtgat
     2641 tgaattttat ccgggccaga aactcagtcc tgaagagccg aacgtaaaat gggaccacaa
     2701 tgcttctccc gaaaccgtcc agaaggtgaa ggaccatctc gcgaaatatg ggatcaaggc
     2761 cgtcaattat ggcgtggtcg gcattcccaa ggaagaagag ggcgcgcgca agatttttga
     2821 gtttgccaaa accatgagct tgcacggcgt cacgaccgaa tcggtggacg cgattgatac
     2881 cattgaaaag ctcgtgaagg aatacgacat caaggtcggt ttccatgacc atccgaaacg
     2941 ccacaatgat cccaattacc gcatgtggga cccgaattat atcttgtctg ttgtgaaaga
     3001 ccgcgacccg cgcattggtt cgtgcgcgga cacgggccat tgggtgcggt ctggtttgaa
     3061 gccggtggat tgcctgcgga tcttgaaagg ccggatcatc agcagccatc tcaaggattt
     3121 gaatgagatg agccatggtg cgcatgacgt tccgtacggc gagggcgtct cggacattcc
     3181 cggcatcttg gaggaattga aagcgcaagg attcgacggc aacatttcca tcgaatacga
     3241 aacggattgg accagctccg tgccggaagt tgcgcaatgc atcggatttg tgcgaggcta
     3301 tggaacggtg aagaattggt gaacgcggca aatatctaaa ccaaccatga aaactgaaat
     3361 acaaccttat ctgttcttta gtgggcgctg cgaagaggcg ctcgaattct ataaaaccgc
     3421 tcttggcgca actgtggact ttatgatgcg ttacaaggaa agtcccgaac cgcctccgcc
     3481 gggcagagtt cctcccggat ttgaaaacaa aatcatgcac gccacgttcc gcatcggcac
     3541 aaacatcctc atggcttcgg acggatgcga agaaggcggg aagttcgagg gcttcagtct
     3601 ggcgatttct gtaccgacgg aagccgaggc caaacgcgcc ttcaccgcgc tctccgccgg
     3661 cggcaaagtg gatctgccgt tgaccaagac attttggtcg ccctgcttcg ggatgctcac
     3721 cgacaagttc ggcatcggct ggatggtcag tgtgatgggg caacagccct gagttgacag
     3781 ccaataaccg cgaaaagtct cgcaaaacgg atctgcaaac cgtttttggg agatttaatt
     3841 gttatttgtc ctctttgctc ctacaacacc caaaattcct gaaaaaggcc attgtttctt
     3901 tcccatcagt gtgcctattc tacccgctcg ttgtgaagat tctgcgcaac ttttgccgct
     3961 gacggttgtt aagcagagtt gaagacgaag catctataaa aaaacaatgg gttttgcatc
     4021 gctcagagag ttttttgccc aagcggcctc ggtcactccc gaccagttcg aggagtggaa
     4081 caaggcttgg cgtgtggctg ttgacaacgg ttcccaggaa tcgttgctca ccttcatttc
     4141 acgtgaaaaa ggcatcgccg aggatgtctt cctccagcaa ttggccaagg cgttgaactg
     4201 gcctttcctg gaaccggcca agcgcgatgt cgtgcccgaa gcgcgccaaa aaatcccgac
     4261 caaagttgcc ttccagtatt ccgtcctgcc aacgcagttc gagaatggcg aactccaggt
     4321 cgtcatcagc aatcctttcg acgccgccat gatgaacgcc gtgcggttca acgccggttg
     4381 cccggtcacg tttgcgctcg cggcaaagat tgaaatcgaa aaggcgctga aaaagtatta
     4441 cggcgttggc gccgaaacgc tcgatgaaat gtccaaggac gagccgatcg aactgctcgt
     4501 cggcgaggac aaagaaatca ccgaaggcga ccaggaagcg agcgtcatca agttcgtcaa
     4561 ccagatcatc tgggaagctt tcaaggaccg cgcgacagac atccattttg aaccggcgga
     4621 agatgagttg cgcatccgtt accgcattga cggcatcctc caccagacgc cgatgccgcc
     4681 gcagttgaag cgctaccagg cggcattgat ttcccgcatc aaggtcatgt cgggaatgaa
     4741 catcgccgag aaacgcctgc cacaggacgg acgcatcaac gtccgcatca agggcgagga
     4801 aatcgacatc cgcgtttcca cggtgccgac ggtttacggc gagagcgtgt ctctgcgttt
     4861 gctcacgcgc ggcaaaatct ttttgagcct ggaaaaactc ggcttttcgc agctcgaaga
     4921 aaacgccatc cgcgaaatca tcctcaagcc gcacgggatt ttcctcgtca caggtccgac
     4981 tggttccggc aaatcgactt ccctctatgc ctttttgagc agcatcaatt ccgtccacaa
     5041 gcgcatcatc acgatcgagg agccggtgga atatgaattg aaaggcatca accagatcgc
     5101 ggtgcgctcg gacatcgggc tgacgttcgc gatgggtttg cgccatattt tgcgacagga
     5161 cccgaacgtc atcatggtcg gggaaatccg cg
//
LOCUS       DASZMM010000028         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1389053, whole genome shotgun sequence.
ACCESSION   DASZMM010000028 DASZMM010000000
VERSION     DASZMM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389053"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VG754_00655"
     CDS             <1..616
                     /locus_tag="VG754_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF17742.1"
                     /translation="YGGNQKTCYRLALEEGADIVIMIHPDYQYTPKLIPAMVSIIGNG
                     LHPCVLGSRILGGYSLKGGMPVWKYIANRFLTAAENILLGAKLSEYHTGYRAFSRELL
                     ERVSVAHNSEDFVFDNQMLAQILWHGYTIGEVSCPTKYFKEASSINFRRSTKYGFGCL
                     STGLSFRLAKMGLWKSKLFPIKAKKVETPASKLEEKLNHQSSDQ"
     gene            complement(664..927)
                     /gene="rpsT"
                     /locus_tag="VG754_00660"
     CDS             complement(664..927)
                     /gene="rpsT"
                     /locus_tag="VG754_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWF17743.1"
                     /translation="MPNTKSAERRMRNSVRKQVHNRSINTRLHTLEKRYLQLLGAGKK
                     EDASKALVEVTSALDKAAKTGTVHKSRASRKKSRLTLQLNKLK"
     gene            1149..2072
                     /locus_tag="VG754_00665"
     CDS             1149..2072
                     /locus_tag="VG754_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWF17744.1"
                     /translation="MRLSYLIILIVMNFFWAGSLSIYKALAGYLQPGEIVTLRFGLAA
                     LILAALWPWLPGKAPRGKDLVKVVVMGLIVFMLGHRIQVFANKLQAAGDSSVLMAMEP
                     ILTSVAAAIFLHEHIGPRRWMGFALGLFGVALLNGFLRSNFTWGGLGISLLFISSFLC
                     EAVYSIMGKPLLERAGMMKILALALIFGTIGNLLLDGPQTFAAVRIMPLHYWWLIFYM
                     ATICTSIGYAIWFVVIKETDVNVAAMTIFAQPVAGVALAGLWLHEPLHWGQFWGSVAI
                     VAGLVLGLSRQIKAELLATVKHSPASAPADG"
     gene            complement(2298..2777)
                     /locus_tag="VG754_00670"
     CDS             complement(2298..2777)
                     /locus_tag="VG754_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17745.1"
                     /translation="MKLLPPYRSAVEMECAQGQRLEWWEHEPESGQQWFRAVEPSELC
                     GHCWQSSTCPRHFEFPAGRHETLFGLLPLGSRVAQRLLQQVRPWIEPAQSFEKNQLGL
                     GQMFFNSLRLTWQMSLWADSAVLLRTMAWLDQPADASLLAGLMPCQLELGLPLEKNG"
     gene            complement(2779..3540)
                     /locus_tag="VG754_00675"
     CDS             complement(2779..3540)
                     /locus_tag="VG754_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17746.1"
                     /translation="MSNILPPVGTNLLLESVCGYVPDDFIASLCARDFTGGRRHALSA
                     PQLWRVHLLALLTPTHSLNLVVAQLSEQPGWRRFARLRRVYPTARMLHEFRQQVGIDG
                     LRAINRHLLERLLRRQGLQPQAVALMDATDLPAACSGFKKKFRALHGNTCRTWRTYAQ
                     DRPKPLVCKLQEAHVAAVVADIPSFGDSGAAGQLGYPSQCIRRRPAGAKFALVSPEFG
                     LVAGHCGGGHGLPVSRQQAASAGRLADSGGDQTAF"
     gene            complement(3996..5117)
                     /gene="waaF"
                     /locus_tag="VG754_00680"
     CDS             complement(3996..5117)
                     /gene="waaF"
                     /locus_tag="VG754_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWF17747.1"
                     /translation="MEWWSIGVGFCTPIFQSDICGQLKNVEKLKILILKPSSLGDVVH
                     ALPVLRLLKLHQPHSEIFWWLESSLVPLLADDPDIAGIFSFQRKSWSASSNWAQHFDC
                     VRRMRRERFDLAIDLQGLARSGLFTWLANAETSIGLDNPREGAREGARLYYDVLTPRC
                     AAGTHAVDRYLAVLPQLGVPVHEKFQWLPEHPQIAAQVREKWQSDSARWIALLPGARW
                     NNKRWPVENFAEVVRGLSFAQPGMKFVILGGNADCALGKMVASANPARCLDLTGQTSL
                     LEMLECLRLSELVITNDTGPMHIAAALRKPIVSLFGPTDPTGTGPYGQQEWVLQTRGL
                     SCVPCMKSTCAYHEPLACLRAITPAMVCEKALQRLNHSR"
     gene            complement(5124..>5768)
                     /locus_tag="VG754_00685"
     CDS             complement(5124..>5768)
                     /locus_tag="VG754_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWF17748.1"
                     /translation="CVGDVVGYGANPRECLKIIRDMGMPVVKGNHDEYCSIEEDLDGF
                     NPHAAEAVNWTRAQLTQDERQWLRDLKYFRMVTSFSMVHATLDGPQRWGYVFNKLDAA
                     ASFPYQNTAVCFFGHTHVPLAFIREPNAAGQPIVKGGTYSKFKVDPNKKYFVNVGSVG
                     QPRDNNPKAAYVVYDVDEGTIELRRLDYDIASAQKKILDAGLPPRLAERLAYGK"
BASE COUNT         1224 a         1580 c         1635 g         1329 t
ORIGIN      
        1 ttacggcggt aaccaaaaaa cctgctaccg gctggcattg gaggaagggg cggacatcgt
       61 catcatgatc cacccggatt atcaatacac gcccaagctc atcccggcga tggtttccat
      121 catcggcaac ggcctgcatc cttgtgttct cggcagccgc attttgggcg gatattcctt
      181 gaagggcgga atgccggttt ggaaatatat cgccaaccgt tttcttaccg cggccgaaaa
      241 cattttgctc ggcgcgaagc tttcggaata ccacaccggc taccgggctt tttcgcggga
      301 actgctcgaa cgcgtttctg tggcccacaa ttccgaagat ttcgtctttg acaaccagat
      361 gctcgcccaa atcctctggc acggttacac catcggcgaa gtgagctgcc cgacgaaata
      421 tttcaaggaa gcatcgtcca tcaacttccg ccgaagcaca aaatacggtt tcggatgtct
      481 ctcgacggga ttgagtttcc ggctggccaa gatgggtttg tggaaatcca aactgtttcc
      541 catcaaagca aaaaaagtcg aaactccagc ctcgaagctc gaggaaaaac tcaatcacca
      601 aagctccgac caataaggat tggtctttgg aaaacttctg tggaggccag ctcttggctc
      661 ttcttacttc aacttgttga gctgaagcgt caggcgggat ttcttgcggc tggcgcggga
      721 cttgtggacg gtgccggtct tggcggcctt gtccaacgct gaggtcactt ccacgagggc
      781 cttggaagca tcttcttttt tcccggcgcc gaggagttgc aaatatcttt tctccagcgt
      841 gtgcaaccgc gtgttgatgc tgcggttgtg gacctgtttg cggacgctgt tccgcatccg
      901 gcgttcggca gacttcgtat tcggcataat tggttcgatt taacgattta gagcgtgtaa
      961 ccgtagccga atgcaggtta atgtcaacgg cttgttgcgc ccttttttca atgcacaaat
     1021 ttgctaaggc gttataataa atcacgcgaa aatcgaactt aatccgctgt cgggcatcta
     1081 aacaccggcg tttgcaggga cggcttgaca ggcttcttgt gagtggtttc tgatttgccc
     1141 ttttccgaat gagattgtcg tatctgatca tattgatagt gatgaatttt ttctgggcag
     1201 gctccttgtc catctacaag gcgctggccg gttatctaca gcccggagaa atcgtcacgc
     1261 tgcgcttcgg gctggccgcc ctgatcctgg ccgcgctctg gccgtggctt cccggcaaag
     1321 cgccgcgcgg aaaggacctg gtcaaggtgg ttgtgatggg cctcatcgtt ttcatgcttg
     1381 gccatcgcat ccaggttttt gcgaacaaac tgcaggcagc cggcgattca tccgtcttga
     1441 tggcgatgga acccattttg acttccgttg ccgcggcgat ttttctgcac gaacacatcg
     1501 ggccgcgccg gtggatgggt tttgcgctgg gactgttcgg cgtggcgttg ctgaacgggt
     1561 ttttgcgcag caatttcaca tggggcgggt tgggcataag tctgttgttc atttcttcgt
     1621 ttttatgcga ggcggtctat tccatcatgg gcaagccgtt gctggaacgg gcgggcatga
     1681 tgaagatcct cgcgctcgcg ctgatatttg gcacgattgg caacctgctg ctcgacggcc
     1741 cgcaaacttt cgcggcggtg cgtatcatgc cgttgcatta ttggtggctg atattttaca
     1801 tggcgacgat ctgcacttcc atcggttacg ccatctggtt tgtggtcatc aaggaaaccg
     1861 acgtgaacgt ggccgcgatg accatcttcg cgcaaccggt ggcgggagtg gccttggctg
     1921 gtttgtggct gcatgagccg ttgcattggg gacaattttg gggcagcgtc gcaatcgtgg
     1981 ccggtctggt gcttggattg tcgcggcaaa tcaaggccga actgctggcg acggtgaaac
     2041 attcgcctgc ttccgcaccg gcggatggat gacaacgctg aacatgggtt tcaccgcaga
     2101 gacgccgaga cgcggagatt gcagcagtag ctgaaaacgt aagttttcag atgatggggc
     2161 gagcattgcc gtgcggaaac accccgcgag ccgaaaatat tttttccatt ttagccttaa
     2221 ttatccttaa ttagagggtg ttccgaaagt cactttgagg aagtgcgagt ttattggtga
     2281 agcagcggat ttctcattta tccgtttttt tccagcggca agccaagctc caactggcac
     2341 ggcatcaaac cggcaagtag cgaggcgtcc gccggttgat ccagccaagc cattgtgcgc
     2401 aacagcacgg cactgtcggc ccagaggctc atctgccaag tcagccggag gctgttgaag
     2461 aacatttggc ccaagcccag ttggtttttc tcaaaggatt gcgccggttc gatccacggg
     2521 cgaacctgtt gcaggagccg ttgcgccacg cggcttccca agggaagcaa tccaaagagc
     2581 gtttcgtgtc gtccggcagg aaattcaaaa tggcgcggac aggtggatga ttgccaacaa
     2641 tgaccgcaga gttcggacgg ttctaccgcc cgaaaccatt gttgtccgct ctccggctcg
     2701 tgctcccacc attccagacg ctggccctgt gcgcattcca tttcaacggc actccgatag
     2761 ggtggcagca gcttcatgtc agaacgcagt ttggtcacca ccgctgtctg ccagccttcc
     2821 cgcgcttgct gcttgctgtc tgctgacagg tagcccatgt ccgccaccac aatgcccggc
     2881 caccagccca aactccggcg acaccaatgc aaacttggca ccagcaggcc gccttcggat
     2941 acattggctg gggtaaccca actgaccagc ggcaccagag tcaccgaagg atgggatgtc
     3001 ggcaaccaca gccgcaacgt gtgcttcttg tagcttacaa accagcggct ttggcctgtc
     3061 ttgagcgtac gtccgccaag tgcggcatgt gttgccgtgt aaagcccgga actttttttt
     3121 gaaaccgctg caggcagccg gcagatccgt cgcatccatc agcgcgacgg cttgcggttg
     3181 cagcccctgt cggcgcaaca aacgttccaa cagatgccgg ttgatggcgc gcaacccgtc
     3241 aatacccacc tgctgtcgga actcatgaag cattcgcgct gtgggataaa cacggcgcaa
     3301 ccgggcgaat cgccgccatc ccggctgttc agaaagttgg gccaccacga gattgagcga
     3361 atgggtgggg gtcaaaagag ccagcaggtg aacccgccac agttgcgggg cactcaacgc
     3421 atggcgacga ccgccggtga aatcccgggc gcagaggctg gcgataaaat catccggcac
     3481 ataaccgcac acgctttcaa gcagaagatt ggtgccgacc ggaggcaaaa tattgctcat
     3541 gccggaactc taactcaaac ccgccttcag gacaccccac ttgtatcggc gtagcatccc
     3601 ttagctgagt aacttacaaa cactttcgga acaccctcta taaaggacat ttttttaaaa
     3661 aagcactcta ccgcatcgaa gacggcgatg cggtagagtg aacacatttt ttgtttttgt
     3721 caatagaaca tttacgtatg gggagtgtca ctggggcaca tctcataaat ccctgaattg
     3781 acagagtggg cactccttgc gcgccgcatt ttcgggtgac ggcgggcagc ggagtgcccg
     3841 ccctgccatt tatgagggtt gtgggtattt gtgttattag gtacatgcat ttgtttcggg
     3901 taggactatc atcacatgac ggttcaagga atcccgtggc cgtgagacgg cttggaaaat
     3961 ggattgtgcc tgaatcaaac cgaagtgaag ttgatttacc ggctgtgatt taatctttgc
     4021 agcgcctttt cgcaaaccat ggccggcgtg atcgcacgca aacaggccag cggttcgtgg
     4081 taagcgcagg tgcttttcat gcagggcacg caggaaagac cgcgcgtctg aagcacccac
     4141 tcctgctgtc catatgggcc ggtgccggtt ggatccgtcg gcccaaagag tgagacgatc
     4201 ggtttgcgca atgcggcggc aatgtgcatt gggccggtgt cattcgtgat caccaattcg
     4261 ctcaggcgca agcattcgag catttccagc agcgaagttt ggccggtcaa atcgaggcaa
     4321 cgcgcgggat tggctgaggc aaccattttg cccagggcgc aatccgcgtt tccgcccagg
     4381 atgacgaact tcatcccggg ttgcgcgaac gacaaaccgc gcacaacttc cgcaaaattt
     4441 tccaccggcc agcgtttgtt gttccaacgc gcgcccggca gcaatgcaat ccatcgcgcc
     4501 gaatctgact gccatttctc ccgcacttgc gcggcgattt gcggatgttc cggcagccat
     4561 tggaattttt cgtgaacggg cacgccgagt tgcggcagca cggcaagata ccggtccacc
     4621 gcgtgagtgc cggcggcgca acgcggcgtc aggacatcgt aataaagcct cgcgccctcg
     4681 cgcgcgcctt cgcggggatt gtcgagccca atgctggttt ccgcgtttgc cagccaggtg
     4741 aaaagtccgc tgcgcgcgag gccctgcaaa tcaatcgcca ggtcaaaacg ctcgcgcctc
     4801 atgcgccgga cgcaatcaaa atgctgcgcc caattggaag acgcggacca actcttgcgt
     4861 tgaaaagaaa aaatccctgc gatgtcggga tcgtcggcga gcagcggaac aaggcttgat
     4921 tccagccacc aaaaaatttc gctgtgcggc tgatgaagtt taagcaggcg aaggaccggc
     4981 agcgcgtgaa ccacgtcgcc gagcgaactg ggcttgagga tgaggatctt cagtttttct
     5041 acgtttttta gctgcccgca aatgtcggat tggaatattg gggtgcagaa gccaactcca
     5101 atgctccacc actccaattc ccgtcatttc ccgtaggcga gccgctccgc caaacgcggc
     5161 ggaaggccgg cgtccaggat ttttttctgc gcggaggcga tgtcgtagtc caggcggcgc
     5221 aattcaatcg tcccctcgtc cacgtcgtac accacataag ccgcctttgg attattgtcg
     5281 cgaggctggc cgacgctgcc gacgttgacg aaatattttt tgttcgggtc cactttgaat
     5341 ttggaataag tgccgccctt cacgatgggc tggccggcgg cgttcggttc gcggatgaac
     5401 gccagcggga cgtgcgtgtg gccgaaaaaa cagacggcgg tgttttgata gggaaaactt
     5461 gccgcggcat cgagtttgtt gaagacataa ccccagcgtt gcgggccatc caatgtggca
     5521 tgtaccatgg aaaagctggt gaccatgcgg aaatatttga ggtcgcgaag ccattgacgt
     5581 tcgtcctggg tcaattgcgc gcgcgtccag ttgaccgctt cggccgcatg gggattgaag
     5641 ccatccaagt cctcctcaat cgaacaatat tcgtcgtggt tgcctttgac cacgggcatt
     5701 cccatgtcgc ggatgatctt gaggcattcc cggggattgg cgccgtaacc caccacatcg
     5761 ccgacgca
//
LOCUS       DASZMM010000029         5108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3036754, whole genome shotgun sequence.
ACCESSION   DASZMM010000029 DASZMM010000000
VERSION     DASZMM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036754"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1518)
                     /locus_tag="VG754_00690"
     CDS             complement(73..1518)
                     /locus_tag="VG754_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF17749.1"
                     /translation="MNCQTQLYRNQHPTAVSRRWFLKECGVGLGAIALHTLLGDYASA
                     ASAQPGANANPLAPRQPHFAPRAKNIIYLFMAGAPSHLELFDYKPQLAKFNGTLPPPE
                     LLKDYRTAFINPNSTLLGPKFKFARHGQCGAELSELLPHLAEVVDDIAIVKAMYTDAF
                     NHAPAQILMHTGSQQFGRPSLGAWTLYGLGSESRNLPGFVVFSSGPKGPSGGASNWGS
                     GFLPTVYNGVMFRCQGDPILYLSNPKGVDDRLQRDTLDSVRQLNKQHLAAVGDPEIST
                     RINSFEMAYRMQTSAPDLMDLSKEPKHVLDMYGVEPGKPSFAMNCLLARRLIERGVRF
                     VELFHEAWDQHGNLKADIAKNCQHTDQASAALLKDLKQRGLLEDTLVIWGGEFGRTPM
                     VQGGDDGRDHHPNCFTVWMAGGGVKRGITLGESDDFGFNATTDKVHVHDLNATILHLL
                     GFDHTKLTYRFQGRDFRLTDVHGEVVKKLVA"
     gene            complement(1644..4529)
                     /locus_tag="VG754_00695"
     CDS             complement(1644..4529)
                     /locus_tag="VG754_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HWF17750.1"
                     /translation="MKPRLLLTKFVFAALTLGCALSASFAATTVSPSATNHWAFKAPV
                     RPALPKVRDSKWGRTPVDTFVLAKLEREKLRPSKEAGKATLLRRLSLDLIGLPPTIEE
                     MDAFLADKNPDAWQKQVERLLASPHYGERWGREWLDAARYADSDGFEKDKPRFIWNYR
                     DWVIGAFNRNLPYDQFVIEQIAGDQLPHPTQDQIVATGFLRNSMLNEEGGVDPEQFRM
                     DGMFDRMEAIGKSVLGLTIQCAQCHNHKFDPLTQEEYYKLFAFLNNDHDASTIAYTPH
                     QQELRANLLRQMRDLEEGLRHKTSDWEQRMAAWEDSVRNDQPEWIPLACTNSSGDNGE
                     RFYYFADHSIRAASYAPTKWSATFRATNDLPLIGGFRLEQLTDPDLPCNGPGRSIKGM
                     SALTEFKVEAQDAKQPTNKVNVKLVKATADFENPEKPLEAEFDDKSGAKRIYGPVLFA
                     IDGKDETAWGIDVGPGRRNQSRKAVFIPEKPLTFTNGVILTFHLRQMHGGWNSDDNEN
                     HNLGRFRLSVTSATNAVADPLPAGVREIFKIPREQRTPTQIATVFSYWRTTVPEFKEV
                     NGQIEKLWSQWPEGAPTLTLVARENRNPGDEKRVTRIFTRGDWLKPAHEVNAGVPAFL
                     HQLPPDADDSRLTLAKWLVDSKSPTTARVFVNRLWQSYFGVGLVETPEDFGLRAPEPS
                     HPGLLDWLAVEFMQPSVRAPGEKTIPGKWDIKHIHRLIVNSAVYRQSSRVTPELYERD
                     QYNRLLARGPRFRVDAEIVRDIALSASGLLDEKMGGPSVTPPAPEFIFKPPASYGPKV
                     WNEATGPDRYRRAVYTFRFRSVPYPVLQTFDAPNGDFSCVRRLRSNTPLQALATLNEV
                     VFMECAQSLALHTLEHGGNDRERIAYAFRRCLGREPNESEMQNLLSLLKKDSQYLGEG
                     WVDVPVLATGKNGPIANLPKGCTPTQLAAYTVVSRVLLNLDETITKE"
     gene            4684..>5108
                     /locus_tag="VG754_00700"
     CDS             4684..>5108
                     /locus_tag="VG754_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWF17751.1"
                     /translation="MSKNTFPKLHNAMWPGLVGKGSPGAEPSIDLDTMLNLTAAAEVN
                     GVKFDGVDLFLFDPHVSIDSSADDLKKLAEKIRAKGFVVGSVVAPVWPPTGGGSAMGN
                     DEERGNFVKQVRKACGIASSLRELGVRPYGIVRIDSACGV"
BASE COUNT         1070 a         1422 c         1502 g         1114 t
ORIGIN      
        1 gcggtcttgg attgccgcag aaatcccgag tggataaaaa agaagctaag ttcaatgtga
       61 ttgaacggag ccttacgcca ccaacttctt caccacctcg ccatgcacat ccgtaaggcg
      121 aaaatcgcgg ccttggaaac ggtaggtcaa tttcgtgtgg tcgaagccga gcagatgcag
      181 gatggtcgcg ttgaggtcgt ggacgtggac tttgtcggtg gtcgcgttga aaccgaaatc
      241 gtctgattcg ccgagcgtga tgccgcgttt cacgccgccg ccagccatcc agacggtgaa
      301 acaattcgga tgatgatcgc gtccgtcatc gccgccttgc accatcggcg tgcgcccgaa
      361 ttcgccgccc caaatcacca gcgtatcttc gagcaacccg cgttgtttca aatccttcaa
      421 caacgcggca gatgcttggt cggtgtgctg gcagtttttt gcgatgtcgg ccttgagatt
      481 tccatgctgg tcccaggctt catgaaaaag ttcgacgaac cgcacgccgc gttcgatcaa
      541 acgccgcgcc agcaaacaat tcatcgcgaa cgaaggtttg cccggctcga cgccatacat
      601 gtcgagcaca tgcttcggct ccttggaaag atccatcaag tccggcgcgc tggtttgcat
      661 ccgatacgcc atctcgaacg aattgatgcg cgtcgaaatt tccgggtcgc cgaccgccgc
      721 aagatgttgt ttgttgagtt ggcgcaccga atcgagcgtg tcgcgctgga gccggtcgtc
      781 cacgcccttg ggattcgaga gataaaggat cggatcgccc tggcagcgga acatcacgcc
      841 attatagacc gtcggcaaaa atcccgaacc ccaattcgac gcgccgccgc tcggcccttt
      901 tgggccggaa ctgaaaacca caaaccccgg caggttccgc gattcgctgc caagcccgta
      961 aagcgtccat gcgccgaggc tcgggcggcc aaattgttgc gagccggtgt gcatgaggat
     1021 ttgcgcgggc gcgtggttga aagcgtccgt gtacatcgcc ttcacgatgg cgatgtcgtc
     1081 cacgacctcg gcgaggtgcg gcaacaattc ggaaagttcc gcgccgcatt ggccgtgcct
     1141 tgcgaatttg aacttcggcc ccagcaatgt ggaattcgga ttgatgaacg ccgtgcggta
     1201 atctttcaac aattcaggtg gcggaagcgt gccgttgaat ttcgcgagtt gcggtttgta
     1261 atcgaacaat tcgagatggc tcggcgcgcc agccatgaac agatagatga tgtttttggc
     1321 gcgaggcgcg aagtgcggct ggcgcggcgc gagcgggttg gcatttgcgc cgggttgcgc
     1381 ggacgcggcg gacgcgtaat cgcccaaaag ggtgtgcagc gcgatggcgc cgaggccgac
     1441 gccgcattcc ttcaagaacc agcggcgcga aaccgcggtt ggatgctggt tgcggtaaag
     1501 ttgcgtttgg caattcataa attccttaat ccgcaggagc cgacgtgagg ttccatttgg
     1561 atttcaaatt tgaaatttca gatctaatgc gagcctcctt aagtcggctt ctaccgggaa
     1621 atatttagat gcgcgcatga atttcattcc ttcgttatcg tttcatccag attcaacagc
     1681 acgcgcgaga ccacagtgta tgcggcgagt tgcgtcggcg tgcagccctt cggcaaattc
     1741 gcaatcgggc cgttcttgcc ggtcgcaagc accgggacat ccacccagcc ttcgccgaga
     1801 tattgcgaat cttttttcag caacgaaagc aagttttgca tctccgattc gttcggttcg
     1861 cggcctagac agcggcgaaa agcgtaagcg atgcgttccc ggtcgttgcc gccatgttcc
     1921 aacgtgtgca aggcaagcga ctgcgcgcat tccatgaaaa cgacttcgtt caaagtcgcg
     1981 agcgcttgca acggcgtgtt cgagcgcagg cggcggacgc aggaaaaatc gccgttgggc
     2041 gcgtcaaaag tttgcaggac agggtagggg actgaacgga aacggaacgt gtaaaccgcg
     2101 cgccgatagc ggtcggggcc ggtcgcttcg ttccagactt tcggcccgta actcgcgggc
     2161 ggcttgaaga taaattccgg cgcgggcggc gtcacgcttg ggccgcccat tttttcatcg
     2221 agcaacccgg aagctgacaa cgcgatgtcc cgcacgattt ccgcatcgac gcgaaaacgc
     2281 ggcccgcgcg cgagcaggcg gttatattgg tcgcgctcat aaagctccgg tgtcacgcgc
     2341 gaagattgcc ggtaaaccgc gctgttcacg atgagacggt ggatgtgttt gatgtcccat
     2401 ttgccgggaa ttgttttttc gcccggcgcg cggacggaag gttgcatgaa ttccaccgcg
     2461 agccaatcga gcaaacccgg atgcgaaggt tcaggcgcgc gcaaaccaaa atcttccggc
     2521 gtctccacca acccaacgcc gaaataggat tgccagagcc ggttcacaaa aacgcgggcc
     2581 gtcgtcggcg atttggaatc gacaagccac ttggcgagtg tgagccggct gtcgtctgca
     2641 tcgggcggca attgatgcaa aaatgccggc acgcctgcgt tcacttcatg cgcgggcttg
     2701 agccagtcgc cgcgagtgaa tatgcgcgtg acgcgttttt catcgccagg attgcggttt
     2761 tcacgcgcga ccaaggtaag tgtcggcgcg ccttcgggcc attgactcca aagtttttca
     2821 atctggccgt tgacctcctt gaactcggga accgtcgtgc gccaataact gaacacggtc
     2881 gcgatttgtg tcggcgtgcg ttgctcgcgc ggaattttga aaatttcgcg cacgcctgcc
     2941 ggaagtggat ccgccacggc gttcgtggcg ctggtgacgc tcaggcgaaa gcggccgaga
     3001 ttatgatttt cattgtcatc ggaattccat ccgccatgca tttgccggag atgaaaagtc
     3061 aaaatcacgc cattggtgaa cgtgagcggc ttttcgggga tgaaaaccgc cttgcgcgat
     3121 tgattgcgac ggcccggccc gacatcaatg ccccaagccg tttcgtcttt gccatcaatc
     3181 gcaaacaaca ccggcccgta aatgcgtttc gcgcctgact tgtcatcgaa ctccgcttcg
     3241 agcggctttt ccggattctc gaagtccgcc gttgccttca cgagcttcac attgactttg
     3301 ttggtgggct gctttgcgtc ctgcgcttcg actttgaatt ccgtcagcgc cgacatgccc
     3361 ttgatggaac gtcccggccc gttgcaaggc agatcgggat cggtgagttg ttcgagtcga
     3421 aaaccgccaa tcaacggcag gtcatttgtc gcgcgaaacg tcgcactcca tttcgtcggc
     3481 gcgtagctcg ccgcgcgaat ggaatgatcg gcgaaataat aaaaacgctc tccgttgtca
     3541 ccggaagaat tggtgcaggc cagcggaatc cattccggtt gatcgttgcg cacggaatct
     3601 tcccacgcag ccatgcgctg ctcccaatca gatgtcttat gacgcagccc ttcttccaga
     3661 tcgcgcatct ggcgcagcaa attcgcgcgc aattcctgtt ggtgcggcgt gtaagcgatg
     3721 gtcgaggcgt catggtcgtt gttcaaaaac gcgaacagct tgtaatactc ctcctgtgtg
     3781 agcggatcga atttatggtt gtggcattgc gcgcattgga tggtcaggcc gaggacgctt
     3841 ttgccgatgg cttccatccg gtcaaacatc ccgtccatgc ggaactgttc cgggtcaacg
     3901 ccgccttcct cgttcaacat cgagttgcgc aaaaaaccgg tggcgacgat ttggtcctgc
     3961 gtgggatggg gcaattgatc gcccgctatc tgctcgatga cgaactggtc gtaaggcagg
     4021 ttgcgattga acgcgccgat gacccaatcg cggtaattcc agatgaaacg cggcttgtcc
     4081 ttttcgaaac cgtccgaatc ggcataacgc gcggcatcga gccattcgcg tccccaacgc
     4141 tcgccataat gcggcgacgc cagcaaccgc tcgacctgct tttgccaggc atcgggattt
     4201 ttgtcggcga gaaacgcgtc catttcctca atcgtcggcg gcaagccgat gaggtcgagg
     4261 ctcaaacggc gcaacaaagt ggctttcccg gcttccttgg atggccgaag tttttcgcgc
     4321 tccagcttgg caaggacaaa agtatccacc ggcgtgcggc cccattttga atcgcggact
     4381 tttggcaatg cgggacgaac aggcgctttg aaggcccaat gattggtcgc agacggcgac
     4441 actgttgtgg ctgcgaatga ggcagaaaga gcgcagccga gcgtcagcgc ggcaaacaca
     4501 aatttcgtca gcagcaagcg cggtttcacg tcagggtttc gcaagcgaca acgggtttta
     4561 atcaacaaac tttagccgta attcaatctt tccacaatag aactttgccc taagtccagt
     4621 cgaaaaggtc ttgtcgcggg cggggctttg gggcattcat aattgcaaat cacaagacgc
     4681 accatgagca aaaatacttt cccaaaactg cacaacgcca tgtggcccgg attggtcggc
     4741 aagggcagcc ctggcgctga accttcgatt gacctggaca cgatgctgaa tctcaccgcc
     4801 gcggcggagg tcaacggtgt caagttcgac ggcgtggacc tgtttctatt cgatccgcat
     4861 gtcagcattg attcgagcgc ggatgatttg aagaaactcg cggagaaaat tcgcgcgaag
     4921 ggctttgtgg tcggttcggt ggtcgcgccc gtttggccgc cgacgggcgg cggttcggcg
     4981 atgggcaacg acgaagaacg cggaaatttc gtcaagcaag tgcgcaaggc ttgcggcatc
     5041 gccagcagtt tgcgcgagtt gggcgtgcgg ccttacggca tcgtgcggat tgattcggct
     5101 tgcggcgt
//
LOCUS       DASZMM010000030         6351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3038642, whole genome shotgun sequence.
ACCESSION   DASZMM010000030 DASZMM010000000
VERSION     DASZMM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3038642"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VG754_00705"
     CDS             <1..588
                     /locus_tag="VG754_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF17752.1"
                     /translation="TAASILEEVERLARIVEGLFAISRLDAGEAQKQTAPFDLANLAI
                     STAEQMCLLAEDKNISITSDAPDEVIVEGDRSRLKQVVVNLLDNAIKYTPEGGKIRLS
                     VHTRDNKAVLEVEDNGIGIPAGAQAHIFERFFRVDKARSRELGGAGLGLSIVKSICAA
                     HGGQVDVRSADGQGSRFTVELPLANAVKMEIKHGN"
     gene            626..1798
                     /locus_tag="VG754_00710"
     CDS             626..1798
                     /locus_tag="VG754_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF17753.1"
                     /translation="MSSPEPILDPSSKAPGSQMRNVCLVLGVLLVLVIAAIFWKRSTA
                     STAESTAPAVVGVAKVTRADLFNEVTIPAEFRPYVEVELHAKVAGYVDQMNVDFGDKV
                     KSGQLLATLEVPELRDQLHNAMAMQQKAEADYTNANLLYNRLQTVNEQHPNLVAQQEL
                     DDAQAKQLSANAAIAAAKAEVERYQTLTNYTRIVAPFDGVITRRSADPGALIQAGTSS
                     ETQPLLRVSDNYHLRLDFPVSVQYVKDIHPDEPVQVRVESLGGKNFTGKISRFTQKVN
                     EDTRTMTVEVELPNPDLQIVPGMYAAVQLKVQQHSDVLSVPIQAVAGEKEPAVYVVND
                     KSEIEPRPVTVGVESPDKCEIVSGLKEGELVMIGNRSLVQPGQKVETKIVTEPAMP"
     gene            1795..2499
                     /locus_tag="VG754_00715"
     CDS             1795..2499
                     /locus_tag="VG754_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HWF17754.1"
                     /translation="MKTTHAKLSAEQLEALRGLDACTVANAIETFHERLRNEGFANDS
                     IHCLFPELKPMVGYAATVKIRGSAPPTADRPYPDRTDWWDYINSLPLPRVVVVHDVST
                     RVGLGSLLGAVHVNILRALKCVGAVTNGSARDLPSARDLKFPLFTGGVSVSHAYVHIV
                     EFGRPVEIAGLKINSGDLLHGDLHGIQSVPLDIAARIPETAARIQKNEQELIALCQSP
                     DFSLEKLRAAVANHHF"
     gene            2502..5672
                     /locus_tag="VG754_00720"
     CDS             2502..5672
                     /locus_tag="VG754_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF17755.1"
                     /translation="MSKFALRYPYLIIVISLIVCVVGLTSLLRMPVDLFPPIKIPVVV
                     VATFYSGMPPEQIENDITGQFERMFTLAPGIDHMESRSLTGVSLIKIYFQAGSDADSD
                     ANSIANLAMSSLRYMPPGTLPPIVMKFDASSLPVCLVTLKGQGLSESQLRDLGQFTIR
                     NQMAGVPGASVPPAFGGRYRQIMVYVDPLKLEAHDLSVMDVVRSVNEANLILPAGDVK
                     IGPRDYNVYANSQINDMKDINLLPLKTVGQASVMVADVGKAQDASQIQVNKVRVNGQS
                     SVYLPVLKQGGDANTIAVVNGVKSVVSKLLDVPKQLVTEVVFDQSVFVKSAIENLVHE
                     GLIGLVLTGVMILVFLGSMRGTAAVFLSIPLSALAAFIALALGGSTINTMVLGGLALA
                     FSRLIDNSVVVLENIFRHLEMGEPPAVAAERGGQEVALPVLAATLTTVIVFFPVTFLY
                     GVSRYLFSALALGVVLSLFASYFVAMTVVPLFCAHWIKIHGNHEVDDDTLTYDKNHRE
                     KLTWGARFNRWFNLKFRRMLGRYDGALALSLARPMTTVLGITGLFILSLCLYPAIGKA
                     YFPRTDPGQFVINLKAPSGTRLELTDELVGRVEQTVRDVVPKSELKIIVSNIGITPGF
                     SSILTPNSGPHTAFVQVGLNDERKRSSFDYMNLVRARLRRELPQVSAYFQTGGLVDAI
                     LNQGMPAPLDIQVGGMDTKKDHEIAVEIARKVTALPAVSDVLVPQDVDYPALKVDIDR
                     ERASELGLTTRELVDSLITGLTSDGMIAPSYWIDPKTGNNYLLTVQYPENRVQNIADL
                     GSMPLRAPRLKKPTRLDSVVHFSHVEAPTEVDHYQIRRTIDVYVSPKGEDLGRIYNDV
                     QKIVGETKLPPNTTITVRGAVQAMRGSFQSFSLGLLLSTLLVYLILVAQFRSFTDPFL
                     ILLAVPTGLTGVLMMLFACGTTLNVMSLMGIVMMVGIVVSNSILIVEFTNRLRAEGRS
                     VREAVALACRVRLRPVLMTSMATLIGLIPMAAKLGAGGEAYAPLAQAIVGGLAVSVVL
                     TMFIVPAAYFLVYRRESEASNPEVSA"
     gene            5669..>6351
                     /locus_tag="VG754_00725"
     CDS             5669..>6351
                     /locus_tag="VG754_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF17756.1"
                     /translation="MKRKYLLIAALMGSSLIAALADDVHPLSLKEAQETAVRNHPRIT
                     AAQLIALASKQAVREARSAYFPHIEADVTAASDTASNTRIAAGGLNNPLILERNAEGL
                     VASQLITDFGRTANLTASSKLQASAQEQNAIATRAQILLAVNSTYFATLAAQSVLDVA
                     KQTVATRQLTFDQVSQLATNKLKSGLDVSFAKVDLGQSKLLLANAENDLESQFAVLSN
                     LLGEREQRR"
BASE COUNT         1320 a         1857 c         1815 g         1359 t
ORIGIN      
        1 acggccgcga gcatccttga agaagtcgaa cgactggccc ggattgtgga gggacttttt
       61 gcgatttcac ggcttgatgc cggcgaagcg caaaaacaaa ccgcgccttt tgatctcgcc
      121 aaccttgcca tcagcaccgc cgaacaaatg tgcctgctcg cggaggataa aaacatttcg
      181 ataacctccg atgcgccgga cgaggtcatc gtggaaggcg accgttcgcg attgaaacag
      241 gtggtggtaa acctgctgga caacgccatc aaatatactc ccgaaggcgg caaaatccgc
      301 ctctctgttc acacccgcga caacaaagcg gtgttggaag ttgaggacaa tggcatcggg
      361 attcctgccg gggcgcaggc acatatcttc gagcggttct tccgcgtgga caaggcgcgt
      421 tcccgggaat tgggcggcgc cggcttggga ctttcaatcg tcaaatccat ctgcgccgct
      481 catggcgggc aggtggatgt ccgcagcgcc gacggccaag gcagccgctt cacggtggaa
      541 ctgccgctcg caaacgctgt aaaaatggaa atcaaacatg gaaactgaac acccaacgcg
      601 accccttaag ccgaatggag caccgatgtc atctcctgaa cccatccttg atccaagttc
      661 caaagcgccc ggctcgcaaa tgcgcaatgt atgtctggtc ttgggcgtac tgctcgtgct
      721 tgtgattgcc gcgatctttt ggaaacgctc gacggcctcg accgctgaat caactgcgcc
      781 cgccgttgtc ggcgtggcca aggtcacgcg cgcagacctt ttcaatgaag tgaccatccc
      841 cgccgaattc cgcccttacg tcgaagttga attgcacgcg aaagtcgccg gttacgtgga
      901 ccagatgaat gtggatttcg gtgacaaggt caagtccggc cagcttctcg ccacgctcga
      961 agtgccggaa ttgcgcgacc agcttcataa cgcgatggcc atgcagcaaa aagccgaggc
     1021 ggattacacg aacgcaaacc tgctctacaa tcgtttgcag accgtgaacg aacagcatcc
     1081 caacctcgtc gcgcagcagg aattggacga tgcgcaggca aagcaattgt ccgcgaatgc
     1141 cgccatcgcc gcagcgaagg ctgaagtcga gcgttaccaa acgctcacca attacacgcg
     1201 catcgtcgcg ccattcgacg gtgtcatcac gcgccgctct gcggacccgg gcgcgctcat
     1261 ccaggccggc acttcttcgg agacgcaacc attgctgcgt gtttcggaca attatcatct
     1321 gcggctcgat tttcccgttt ctgtccaata cgtgaaagac atccatcctg atgagccggt
     1381 ccaagtgcga gtggagtcgc ttggcggcaa aaatttcact gggaaaatct cacggttcac
     1441 gcaaaaagtg aatgaagaca cccgcaccat gaccgtggag gtcgaactgc cgaatccgga
     1501 cctgcaaatc gtccccggca tgtacgcggc ggtgcaattg aaagtgcagc agcattccga
     1561 tgttctttcc gtgcccatcc aggcggttgc aggtgaaaaa gagccggccg tttacgttgt
     1621 caacgacaag tctgaaatcg agccgcgccc cgtcacagtt ggcgtggaat cgcccgacaa
     1681 atgcgagatc gtttccggat tgaaggaagg cgagctggtg atgatcggca accgctcgct
     1741 cgtgcagccg ggccagaagg tggaaaccaa aattgtcacc gaacccgcaa tgccgtgaaa
     1801 accacgcacg ccaaactttc cgccgagcaa ttggaagcat tgcggggctt ggatgcctgc
     1861 accgttgcca atgccattga aacttttcac gagcgtctgc gcaatgaagg tttcgccaac
     1921 gattcaatcc attgcctttt tccggagttg aaaccaatgg ttggttacgc cgccacagtc
     1981 aagatacgcg gttctgcgcc gccgacggca gaccgcccgt atccagaccg caccgattgg
     2041 tgggattaca tcaattcatt gccattgccc cgcgtggtag tcgtccacga tgtgtccacg
     2101 cgcgtcggcc tcggttcgct tttgggcgcg gtgcacgtca acatcctgcg cgcgctcaaa
     2161 tgcgtcggcg cggtgacgaa tggttcggcg cgcgatttgc cctcggcgcg ggatttgaaa
     2221 tttcccttgt tcaccggggg cgtttccgtt tctcatgctt atgtccacat cgtcgagttc
     2281 ggcaggccgg tggaaattgc cggtttgaaa atcaactccg gcgatttgct ccatggggac
     2341 ttgcacggga tccaatcggt cccgctcgac atcgccgcgc ggattcctga aacggcggcc
     2401 cgcatccaga aaaatgaaca ggagctgatc gcgctctgcc agtcgcccga tttttcgctg
     2461 gaaaaactcc gcgccgccgt cgcgaaccac catttttagc catgtccaaa tttgcgttgc
     2521 gttatcccta tttgatcatc gtcatctccc tcatcgtctg tgtcgtgggg ttgacaagct
     2581 tgctgcggat gccggttgat ttgtttccgc ccatcaaaat tcccgtggtg gttgtggcaa
     2641 cgttttattc cgggatgccg ccggaacaga ttgagaacga catcaccggg caattcgagc
     2701 gcatgttcac gctcgcgccg ggcattgacc acatggaatc gcgctcgctc acgggtgtca
     2761 gtctcatcaa gatttatttt caggcaggct ccgatgcgga ttcggacgcg aacagcatcg
     2821 ccaatctcgc catgtccagc ctgcgttaca tgccgccggg aacgctgccg cccatcgtga
     2881 tgaaatttga cgcgtccagt ttgccggtct gtttggtgac gctcaagggg caaggattgt
     2941 ccgaatcgca attgcgcgat ttggggcaat tcacgatccg caaccagatg gctggcgtgc
     3001 cgggcgcttc cgtgccgccg gcatttggcg gtcgttatcg gcagattatg gtgtatgttg
     3061 acccgcttaa actggaggcg catgacttga gtgtgatgga tgtggtgcgc agcgtgaatg
     3121 aagcaaacct catcctgccc gcgggcgatg tcaaaatcgg tccgcgcgat tacaatgtct
     3181 atgccaacag ccagatcaac gacatgaagg acatcaacct gctgccgctc aaaacagtcg
     3241 gccaggcatc cgtgatggtg gcggacgttg ggaaggcgca ggacgcttcc caaatccagg
     3301 tgaacaaagt gcgcgtcaac ggccagtcct cggtttacct gccggtgttg aaacaaggcg
     3361 gcgacgccaa caccatcgcg gtagtgaatg gcgtcaaaag cgtcgtttcc aaactgctgg
     3421 atgttccgaa gcaattggtc accgaggtgg tgttcgacca atcggtgttc gtgaaatcgg
     3481 ccatcgaaaa tctcgttcat gaaggattga tcggacttgt gctcaccggg gtgatgattc
     3541 tggtgttcct tggcagcatg cgaggcacgg cggcggtatt tctttccatt ccgctctcgg
     3601 cgctggcggc attcatcgcg ctcgcgttgg gcggcagcac catcaacacg atggttttgg
     3661 gcgggctggc gctggcgttc tcgcgcttga ttgacaactc ggtggtcgtc ttggaaaata
     3721 tttttcgcca tctggaaatg ggcgaaccgc ccgccgtcgc agccgaacgc ggcggacagg
     3781 aagtggcgtt gccggtcctt gccgccaccc tgacgacggt catcgttttt tttcctgtga
     3841 ccttccttta cggggtcagc cgttatctct tttcggccct cgcgctcggc gtggtgttgt
     3901 cgctctttgc gtcctatttc gtcgcgatga ccgtggtgcc gctgttttgc gcgcattgga
     3961 tcaagatcca tggcaatcac gaagtggatg atgacacgct gacctatgat aaaaatcaca
     4021 gggagaaatt aacgtggggc gcgcggttca atcggtggtt caacctcaaa ttccgccgca
     4081 tgctcggccg ctatgatggc gcgctcgcac tttccctggc gcgtcccatg acgacggtgc
     4141 tcggaatcac cggcctcttc atcctgagcc tgtgtcttta cccggccatc ggcaaggctt
     4201 acttcccgcg caccgatccg gggcagttcg tgatcaacct gaaagcgccg tccggcacgc
     4261 gattggaatt gacggatgaa ctcgttggcc gcgtcgaaca gaccgtccgc gatgtcgtcc
     4321 cgaagagcga actgaagatc atcgtgtcga acatcggcat cacacccggt ttttcgtcca
     4381 tccttacgcc caattccggg ccgcacaccg cgttcgtgca ggtcgggctt aacgacgagc
     4441 gcaagcggag cagttttgat tatatgaacc ttgtccgcgc gcggttgcgc cgcgaattgc
     4501 cgcaggtcag cgcgtatttc cagacgggtg gcctcgtcga tgcgatactc aaccagggaa
     4561 tgcccgcacc tctggacatc caagtgggcg gtatggacac caaaaaggac cacgaaatcg
     4621 ccgttgagat cgcccgcaag gtgaccgccc tgccggccgt cagcgacgtg ctggtgccac
     4681 aggacgtgga ttatcccgcg ttgaaggttg atattgatcg cgagcgcgcc agcgaactcg
     4741 gtctcacgac gagggaactg gttgacagcc tcatcactgg gctgacttcc gacggcatga
     4801 tcgcgccgag ctactggatc gatcccaaaa ctggaaacaa ttatttgctg acggtgcagt
     4861 atcccgaaaa ccgggtgcag aacatcgccg acctcggctc catgccactg cgcgcgccgc
     4921 gcctcaagaa gccgacgagg ctcgattcgg tcgttcactt ctcacacgtt gaagcgccga
     4981 cagaagtgga ccattatcaa atccggcgga ccattgacgt gtacgtctcg ccgaagggcg
     5041 aggacttggg gaggatttac aacgacgtgc aaaaaatcgt gggcgaaacc aagttgccgc
     5101 cgaacacgac catcaccgtc cgaggcgccg tccaggcgat gcgcgggtcc ttccaaagtt
     5161 tcagcctcgg gttgctgctt tcgaccctgc tggtgtatct gatcctcgtg gcgcagttcc
     5221 gctccttcac ggatccgttc ctcatcctgc ttgcggtgcc caccggactg acgggcgtgc
     5281 tgatgatgct ctttgcctgc ggcacgacgc tcaacgtgat gtcgttgatg ggcattgtca
     5341 tgatggtcgg catcgtggtt tccaacagca ttctcatcgt ggagttcaca aaccggttgc
     5401 gggcggaggg ccgttccgtg cgggaagcag tggcgctggc ttgccgcgtg cgcctgcgcc
     5461 ccgtgctgat gacttccatg gccacattga tcggattgat tcccatggct gcgaaactgg
     5521 gcgctggcgg cgaagcgtat gcgccgctgg cgcaagccat cgtcggcggg ctggcggttt
     5581 cggtggtgct gaccatgttc atcgtgccgg ccgcctattt cctcgtttac cgccgcgagt
     5641 ccgaggcgtc caatccggag gtttccgcat gaaaagaaaa tatttgctga ttgccgcgct
     5701 aatgggaagc agcttgattg cggccctcgc tgatgacgtt catccgcttt cattgaaaga
     5761 ggcgcaggaa actgccgtcc gcaaccatcc gcggatcacc gccgctcaat tgatcgcgct
     5821 cgcttcaaag caggctgtgc gtgaggcgcg ctcggcatat tttccccaca tcgaagcgga
     5881 cgtgacagcc gcgagcgaca cggcgtccaa cactcgcatc gcagcaggcg gattaaacaa
     5941 tccgctcatt ctggaacgca atgccgaggg tttggttgcg agccaactca tcaccgattt
     6001 cgggcgcacg gccaacctta ccgcgagttc gaaattgcag gccagcgcgc aggaacagaa
     6061 tgccattgcg acacgcgccc aaatcctgct cgccgtcaat tccacttatt tcgcaacgct
     6121 ggcggcacaa tcagttctcg acgtggcaaa acaaaccgtg gcaacgcgcc agttgacctt
     6181 cgatcaggtg agtcagctcg ccacgaacaa attaaaatcg ggcctcgacg tgagctttgc
     6241 caaggttgac ctcgggcaaa gcaaactgct attggccaat gccgaaaatg atctggaatc
     6301 gcagttcgcc gtcttgtcga atcttctggg cgaacgcgaa cagcgccgtt a
//
LOCUS       DASZMM010000031         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3038730, whole genome shotgun sequence.
ACCESSION   DASZMM010000031 DASZMM010000000
VERSION     DASZMM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3038730"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VG754_00730"
     CDS             complement(<1..328)
                     /locus_tag="VG754_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17757.1"
                     /translation="MKSLGITLIISGLGFVLAATAETQSARTQETISTPQKLSLTEAK
                     EIAFQRNWDLLAARSGIDMAQAQLIVVKEFPNPALSWSTSKIGSHESATFLGNSFWNR
                     SYDSIAA"
     gene            complement(588..1247)
                     /locus_tag="VG754_00735"
     CDS             complement(588..1247)
                     /locus_tag="VG754_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF17758.1"
                     /translation="MAEKTKVLLVDDHPLVQEWLANLINQQADLEVCGEASNASTALE
                     LVGTAKPAIAVVDISMEGGSGIELIKNIKALHPGVIVIVLSMHDEMLYAERALRAGAR
                     AYIMKREATRKVLLAIRAVLEGKLYVSDKVNAAMAEKFVEGRSAVTDSPVERLSDREL
                     EVFQLLGRGLSTRQISEDLNVSFKTVQAYCARIKEKLNLTNATELLREAIRWHDAQKA
                     E"
     gene            complement(1240..2304)
                     /locus_tag="VG754_00740"
     CDS             complement(1240..2304)
                     /locus_tag="VG754_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWF17759.1"
                     /translation="MFGWIKQFEQYSKPLLMTLSLLLVAIIGLVDWVTGFELSFFVFY
                     LIPVALAVWFVGDVFGVVISLLSVVVWVAGDVSAGAKYSRPFVPVWNALIALAFLFVV
                     VGILRRLRNLQKELEERVRQRTVALTNEMQERTRLETELLGISEREQRRIGHDLHDSL
                     CQHLTGTALAGQVLGEKLSEKSLPEAADAGHLVALVEQAIDLTRTLARGLHPAELEGE
                     GFREAFGELAANITEHFKIPCRFECPKGLRIHAPAGAIHLYRIAQEAINNAIKHSKAT
                     QIVIRLDQDDEVIKLSVSDDGVGLPENARNRNGMGLRIMAYRASMIGGSFGIERVVPH
                     GTRVTCIVPRNGNSSAQTNG"
     gene            2390..3049
                     /locus_tag="VG754_00745"
     CDS             2390..3049
                     /locus_tag="VG754_00745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWF17760.1"
                     /translation="MSSAQHWRPRDAWLFAGFAIIYAFLSAIVIVAPAMVFPSWEGLL
                     RARIISSAFFVFNLCVFLGISVFLSRVKSLSEFKSAFDLTKPRETDVLMSIGIGILLQ
                     FTGIFLFGNLSHLHPTRSFQYDFVPALLVPFFEEPAMRGFVYKSFRNAYGVVPSMLLV
                     VAISLVFHTQVYSSVYRFVMISALNAILCLLKEKRFSLWNCIACHLAFNAVYVGIDRG
                     G"
     gene            complement(3146..>3551)
                     /locus_tag="VG754_00750"
     CDS             complement(3146..>3551)
                     /locus_tag="VG754_00750"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWF17761.1"
                     /translation="YHFKLWFWALKRSKGRQRAIILFEGCTASILIVASIVFCTKIPA
                     GLIYVLLMIMGAWITPLITSYVPHDPNGATELEQTRVFRGKAFSLIALEHLYHLEHHL
                     YPSVPHQNWPKLAKRLDPYFERQGIVPIRGWF"
BASE COUNT          808 a         1002 c          878 g          863 t
ORIGIN      
        1 ctgcggcgat ggaatcgtaa ctgcggttcc aaaaactgtt gccaaggaac gtggcgcttt
       61 cgtgcgaacc aattttcgat gtggaccatg acaacgcggg gttgggaaat tccttgacca
      121 cgatgagttg cgcctgcgcc atgtcaatcc cgctcctggc ggcaagcagg tcccaattcc
      181 tttgaaacgc gatttccttc gcctcagtca gcgataattt ttgcggcgtg gaaatcgttt
      241 cctgcgtacg cgccgattgc gtttccgctg tcgcggccag aacaaacccc aaccccgata
      301 taatgagcgt gatgccaagg cttttcacta gcttttacct taaccaagtc ggggtgaaaa
      361 atctgtgcag ccatgcctaa ttgttttgta cggcatcgtc gtacaaaaaa tgtcgggcgt
      421 gtaatgaacg ggaacaaaga atggccacga aaaggcacag aaggaacaaa agaggactga
      481 gattgaggat tgaagaaagc ggatggaaaa tccattcagc ctctggtcta tcctccatcc
      541 tccattttcc gtcctcagtt ttttgtgcct tctgtgcctt ttcgtggcta ttccgccttt
      601 tgcgcgtcgt gccagcggat ggcttcgcgc agcagttccg tggcgttggt gagattgagc
      661 ttttccttga tgcgcgcgca ataagcctgg acggttttga aactgacatt caaatcctcg
      721 gaaatctggc gcgtgcttaa tccgcgtcca agcaattgaa aaacttccaa ctcgcggtcg
      781 ctaagccgtt cgacaggcga atcagtgacg gcagaacggc cttccacaaa tttttccgcc
      841 atcgccgcat tcaccttatc gctgacgtaa agctttccct cgagcacggc gcgaattgca
      901 agcaacacct tcctggtcgc ttcgcgtttc atgatgtaag cgcgcgctcc ggcgcgcaac
      961 gcgcgttcgg catacaacat ttcgtcatgc atcgagagca cgatcacaat cacgcccgga
     1021 tgcagcgcct tgatgttctt gatgagttca atgcccgaac cgccctccat cgaaatatcc
     1081 accaccgcaa tggccggctt ggccgtgccg acaagttcca atgcggttga agcgtttgat
     1141 gcttctccgc aaacctcgag gtcagcctgt tgattgatca aattcgccag ccattcctgc
     1201 acgagcggat gatcgtcaac caacagtact ttggtttttt cagccatttg tttgcgcgga
     1261 agagttgccg ttgcggggca cgatgcacgt cacccgcgtg ccgtgcggaa ccacccgctc
     1321 gatgccgaag gagcccccga tcatgctcgc gcgatacgcc atgatgcgca gacccatccc
     1381 atttcggttg cgcgcgtttt ccggcaggcc gacaccgtca tcgctgacgc tcaacttaat
     1441 cacctcatca tcctggtcaa gacgaatgac gatctgggtc gccttgctgt gtttgatggc
     1501 gttgttgatg gcctcctgcg caatccgata caaatggatc gcgcccgccg gggcatgaat
     1561 gcgcaagccc ttcggacatt cgaaccggca gggaatcttg aaatgctcgg tgatattggc
     1621 cgccagttca ccgaacgcct cgcgaaaacc ttcgccctcc agttccgccg gatgcaaccc
     1681 gcgcgccagc gtgcgcgtca gatcaatcgc ctgctcgacc agcgccacca aatgcccggc
     1741 atcggcggct tccggaagcg atttctccga caacttttcg ccgagcacct gtcccgccaa
     1801 tgcagtgcca gtcaggtgct ggcacaggct gtcatgcaaa tcatggccaa tgcgccgttg
     1861 ttcgcgttcg ctgatgccca gcaactccgt ttccaggcgc gtccgttcct gcatctcgtt
     1921 cgtaagcgcg acagtccgtt gacgcacccg ttcctccagt tccttttgca gattgcgcaa
     1981 acgccgcaga attcccacga caacaaacaa aaatgccagc gcgatgagcg cattccaaac
     2041 tggcacgaac ggccgggaat acttcgcgcc cgcgctcacg tcacccgcga cccacaccac
     2101 cacgctcagc agcgaaatga ccactccaaa aacatctcca acaaaccaga ccgccagcgc
     2161 gacaggtatc agataaaaca cgaaaaacga aagctcgaag cctgtcaccc aatcgaccaa
     2221 tccgatgatc gccaccagca gaaggctcaa ggtcatcagc agcggcttcg agtattgctc
     2281 aaattgtttg atccacccga acatggtcat caatcatggc gcggaggcgg gtgccgcgca
     2341 agccgcacgt atgttgctat ttgattaaaa attcaaatat aatgcggcca tgagcagcgc
     2401 ccaacattgg cggccacggg atgcatggtt gttcgctggt ttcgcaatta tttatgcttt
     2461 tttatctgcc attgtaatcg tcgcgccggc aatggtgttt ccatcttggg aaggcttgct
     2521 gcgcgcgcgg ataatcagca gcgcgttctt tgtgttcaac ctctgtgtgt ttctcggaat
     2581 cagtgtcttc ttatccaggg taaaatcgct ttcggaattt aagtctgctt ttgatttgac
     2641 caagcctcgt gagacggacg tgttaatgtc cataggaatc ggtattttgc ttcaattcac
     2701 cggaatattt cttttcggga atctttcaca ccttcacccg acacgttcat ttcagtatga
     2761 cttcgtccct gcccttttag taccattttt cgaagaaccg gcgatgcgag ggtttgttta
     2821 caagtctttc cgtaatgcgt acggagtggt tccaagcatg cttctcgtgg tcgcaatcag
     2881 cttggtattt cacacccaag tttatagctc agtctatcgg tttgtgatga tcagcgccct
     2941 aaacgcgata ctttgtcttc tgaaggaaaa acgtttcagc ctgtggaatt gcatagcgtg
     3001 tcatctggcg ttcaacgcgg tatatgtcgg aattgatcgt gggggttgaa atttcagttc
     3061 gcgattcacg cacggtgttc aatttcacaa tccgggacaa ccggcaagca cgcgctcttg
     3121 ggtcgcgcca gtcatgatca aatgctcaga accatccgcg aatcggcaca attccttgcc
     3181 gttcaaaata tggatcgagt cgtttcgcca gcttcggcca gttttgatga ggaactgatg
     3241 gatacaggtg atgttccaga tgatataaat gttccaatgc gatcagggaa aacgctttcc
     3301 ccctgaacac ccgggtctgc tccagttcgg ttgccccatt tgggtcatgc ggaacatagg
     3361 acgtgatcag tggcgtgatc caggccccca tgatcatcaa cagcacatag atcaaaccgg
     3421 cgggaatttt tgtgcagaaa actattgagg ccacaatcaa aatactggcc gtgcagcctt
     3481 caaacaggat gattgcgcgt tgacgcccct tgctccgttt caaggcccag aaccataatt
     3541 taaaatgata t
//
LOCUS       DASZMM010000032         5442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2489425, whole genome shotgun sequence.
ACCESSION   DASZMM010000032 DASZMM010000000
VERSION     DASZMM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489425"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..273)
                     /locus_tag="VG754_00755"
     tRNA            complement(199..273)
                     /locus_tag="VG754_00755"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Val,seq:gac)
     gene            417..1967
                     /gene="purH"
                     /locus_tag="VG754_00760"
     CDS             417..1967
                     /gene="purH"
                     /locus_tag="VG754_00760"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWF17762.1"
                     /translation="MAKIQRALLSVSDKTGLIPLAQTLVQNGVALISTGGTAKALRDA
                     GLAVTDISAHTGFPEMMDGRVKTLHPKIHGGLLFIRGNEKHEAAAREHQIEPIDLVVV
                     NLYPFEQTVAKPDVKLDEAVENIDIGGPSMLRSAAKNHESVTVVVDPADYAEVGKQIS
                     ESGGTTLELRRHLAAKVFARTAAYDAAIAAHLAKEFERVTTNVPHALFINAPRAQSLR
                     YGENPHQAAALYGRFHEYFQQLHGKELSYNNILDLTAAAALIAEFNEDGPTLAILKHT
                     NPCGVGQGTNLREAWDKAFATDKQAPFGGIIAVNRELDASCAEAISEIFSEVIVAPAF
                     NADALALLQKKKNLRLLKVLKSPLSAAPWDVRSVGADSFLWQERDLKQTGAADLKIVT
                     RRKPTESELQAMLFGWRVVKHLKSNAILYAAADRTLGVGAGQMSRVDSSRIAVWKAGE
                     AGLSLKNSVVCSDAFFPFPDGLIAAAEAGATAAIQPGGSVRDAEVIAAADERNLAMAF
                     TGVRHFRH"
     gene            complement(2012..2521)
                     /locus_tag="VG754_00765"
     CDS             complement(2012..2521)
                     /locus_tag="VG754_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWF17763.1"
                     /translation="MKRLITALVIASALVLSACAEESAKLSNVHLCCDKCVKGVDTAV
                     GSVSGVKATCDKEAGTVELTGSDKESVQKAADALVAADHFGTSSDVKLDSATGAKTDK
                     VQTMKISGMHICCAKCVKAIDKAVKSVPGVAGETAAKGVDSFEVTGDFSPKDVMDALQ
                     KAGLTGKAN"
     gene            2875..3267
                     /locus_tag="VG754_00770"
     CDS             2875..3267
                     /locus_tag="VG754_00770"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWF17764.1"
                     /translation="METPPPLSPPPIGSIPPPAPLPPRGNDKLWSILSHLSALIGVGY
                     LVVPLVVYLVMKNESEYVACNAREALNFHISVLIYALCCIPLVFVVIGIPLMMIVGFG
                     SLILSIVAAVKASEGGCYHYPLTIRLVQ"
     gene            3354..4052
                     /locus_tag="VG754_00775"
     CDS             3354..4052
                     /locus_tag="VG754_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HWF17765.1"
                     /translation="MTKTFAELNFPGRLIAVEGLDGSGKSTQIYLLKRWLELQGLKVF
                     FSEWNSSELVKSATSKGKKRELLTPTTFSLIHATDFADRYERQLVPLLRAGYLVLCDR
                     YIFTAFARDTVRGCPPEWVRGLYNFAAHPDLTFFFKAQLEVSLQRILDGRPQLKYFEA
                     GMDMRLSTDPYESFRIFQGRILEQYLGMSTEFNFSVIDANQPIEAQQSVVRELIAAKI
                     DLASFAANHAGTFK"
     gene            4049..4804
                     /locus_tag="VG754_00780"
     CDS             4049..4804
                     /locus_tag="VG754_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HWF17766.1"
                     /translation="MKRIISKPVMPLSQRRVMVPRPSKRRFYGYGIPNVDLQRLAGKL
                     IVIEGADGSGRSTQIARLRDWLEGCGHATVQVGLKRSTLVSEELEQAQNGNILSHTTL
                     SLFYATDFADQLENIILPALKAGFMVLADRYIYTLMARDLVRGMEEAWLKNLYGIALA
                     PDAVFYLNVSPEQLVERNFAKNYALDYWESGMDLGLSREMFDSFLKYQSLMAQEFKRL
                     QSIYGFSIVDGNRTPDEINVELQKKIEAVVTGK"
     gene            5007..>5442
                     /locus_tag="VG754_00785"
     CDS             5007..>5442
                     /locus_tag="VG754_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWF17767.1"
                     /translation="MAEANNKGEYLVGVDLGGTKILAGVFTTSLKCVGRSKMSTKAER
                     GPEAVIERIAHCVQDAVDECDLSLKQVRGIGIGAPGSVDPDSGKVMFAGNLGWKDMPL
                     KRELESHIGLPVFLQNDCNVCALGVHEVELEGKPKNMIGIFLG"
BASE COUNT         1217 a         1430 c         1577 g         1218 t
ORIGIN      
        1 attcgcggca gtcctggtgc cgttgttgcg caagatagcc acgcctgccg gggcatcggc
       61 gggcggtcat tgaattgaat gataacagcc aaaaccaaag aatcgtagga gtcgaggtaa
      121 cgagaccccg atttccgcgc aaaaacgcat gaaagttaga gactcctcac gtcgtcttaa
      181 acgagctgat aagggagatg gtgcgcgata cagggttcga acctgtgacc cctaccgtgt
      241 caaggtaatg ctctaccact gagctaaccg cgccacccaa aagggacggg cattctcgca
      301 aaacccaaag taaatttgca agcgcgaatt cgccactctt tttttgcgtt aaacgggcca
      361 ttatgatttg ccggttgacg ggaggcatgg acttgccgga ttttaacggg aactttatgg
      421 caaaaataca acgcgccctg ctttcggttt cggacaagac cgggctcatc ccgctcgccc
      481 agacgctggt tcaaaacggc gttgcgctca tctcgaccgg cgggacggcc aaggctttgc
      541 gcgatgccgg gcttgcggtg accgacatca gcgcgcacac cggttttccc gagatgatgg
      601 atggccgtgt caagaccctg catcccaaaa tccacggcgg cctgcttttc attcgcggca
      661 acgaaaaaca cgaagccgcc gcgcgcgaac atcaaatcga accgattgat ctggtcgtgg
      721 tgaacctgta tccgttcgag caaacggtcg ccaagccgga tgtcaaattg gacgaggcgg
      781 tcgagaatat cgacattggc gggccgtcca tgctgcggag cgcggccaag aaccacgaga
      841 gcgtgacggt ggtggttgac ccggcggatt acgcggaggt cggcaaacaa atcagcgagt
      901 cgggcggcac aacgcttgaa ttgcgccggc atcttgcggc aaaagttttc gcgcgcacag
      961 cggcttatga tgctgccatc gctgcgcatc ttgcgaaaga gttcgagcgg gtgacgacga
     1021 atgttcccca cgcgcttttc atcaacgccc cgcgcgcgca gtcattgcgt tacggcgaaa
     1081 acccgcatca agccgccgcg ctttacgggc gtttccatga atattttcaa cagctccatg
     1141 gcaaggaact ttcctataac aacattcttg atctgaccgc tgcggcagcg ttgatcgccg
     1201 aattcaacga ggatggtccc acgctggcaa tcctcaagca cacgaatccc tgcggcgtcg
     1261 ggcagggaac aaatctgcgc gaagcctggg acaaggcgtt cgcgacggac aaacaagcgc
     1321 catttggcgg catcattgcc gtgaaccgcg aactcgatgc atcgtgcgca gaagcgatct
     1381 cagaaatttt cagcgaagtc atcgttgcgc ccgcgttcaa cgccgacgcg cttgctttgt
     1441 tgcaaaagaa aaaaaacctg cgcctgctca aagtgttgaa aagtccgttg agcgccgcgc
     1501 cgtgggacgt gcgaagcgtg ggcgcggatt catttttatg gcaggaacgc gatttgaaac
     1561 agaccggcgc tgctgacttg aaaattgtca cacgtcggaa accgaccgag tcggaattgc
     1621 aggcgatgct cttcggctgg cgcgtggtga agcacctgaa atccaatgcc atcctttacg
     1681 ctgccgcaga ccgcacgctc ggcgtgggcg cgggccagat gagccgcgtg gattcgagcc
     1741 ggatcgcggt gtggaaagcg ggcgaagcag ggctttcgct gaagaacagc gtggtttgca
     1801 gcgacgcttt ttttccgttt cccgacggat tgatcgcggc tgccgaagcg ggcgcgaccg
     1861 ctgcgatcca accgggtggt tcagtgcgcg atgccgaagt catcgccgca gccgacgaac
     1921 ggaacttggc gatggccttc accggcgtaa ggcatttccg gcattaaaag aatttgccat
     1981 tacgccgccg cactcgacgc gcgagtctga atcagttggc cttgccggtg agaccggctt
     2041 tctgaagggc gtccatgacg tctttggggc tgaaatcgcc ggtgacttca aaagaatcaa
     2101 cacccttggc cgcagtctcg ccagccacgc cggggacgga cttgacggct ttgtcaatgg
     2161 ccttgacaca cttggcgcag caaatatgca tgccgctgat tttcatggtc tgcactttgt
     2221 cagtcttggc gccggtcgcg gagtcgagtt tcacgtcgct gctggtgccg aaatgatcgg
     2281 cggcgacaag cgcatcggcg gccttttgca cggattcttt gtccgaaccg gtgagttcaa
     2341 cggtgccggc ttctttgtcg caagtggcct tgacgccgga gacggagccg acggcggtat
     2401 caacaccttt gacacatttg tcgcaacaaa ggtggacgtt gctgagtttg gcggattcct
     2461 cggcgcaggc ggaaaggacc agcgcagacg cgatgacaag ggcggtgatt aatcgtttca
     2521 cggttggagt attggttgaa agttgagggt tgattgcttg taaaagcgca cccttagtca
     2581 acaccctgtt ccacgcggcg tcaaggtgga acgggtgttg aaaattgcta caacaggtct
     2641 cttaaaaagg tcgcggctgt gcgaagcgcc agccgcagca acgcgcgaac taccgggcat
     2701 tccaagctgc tgcggctggt tcttgctgac acagccgcgc tccgttctcc gtttatgaga
     2761 tgacttgcag gttaaaaggt tggaatatct ttttgtgtac cgagcaagcc tttaacaatc
     2821 ttgactttaa cggtcattga ctcacattgg aattaatcag gagaaaggac atttatggaa
     2881 acgccgccgc cattatcacc accgcccatc ggctctattc cgccgccagc gccgttgcca
     2941 ccacgcggaa acgacaagct ctggagcatt ctcagccatc tctcggcatt gatcggggtt
     3001 ggttatcttg tcgtgccact ggttgtttat ctggtgatga aaaacgagtc ggaatatgtc
     3061 gcatgcaacg cgcgcgaagc gttgaacttt catatcagtg ttttgattta tgccctttgt
     3121 tgcatcccgc tggtctttgt ggttattgga attccactga tgatgatcgt tggttttggc
     3181 tccctgatcc tgtccatcgt cgcggccgtc aaagcttctg aaggcggatg ctatcattat
     3241 ccgctgacga ttcgtttggt gcaatgatgc cctgatcgcg gggctgaaaa ctcacgttta
     3301 cagctacaaa aacattgttt tcatccggca tcggtttatt ttggatgctg ccgatgacaa
     3361 aaacatttgc cgaattgaat tttccgggcc gcttgatcgc ggtggaggga ttggacggct
     3421 ctggcaagtc cacccaaatc tacctgctca agcgctggct cgaattgcag gggctcaagg
     3481 tttttttcag cgaatggaat tcttcggaac tcgtcaaaag cgccaccagc aagggcaaaa
     3541 aacgcgaact gctcacgccc acgacattca gcctcatcca tgcgaccgat ttcgccgacc
     3601 gctatgaacg gcaattggtg ccattgttgc gcgcgggtta tctcgtcttg tgcgaccgtt
     3661 atatcttcac cgcgttcgcg cgcgacacgg tgcgcggttg tccgccggaa tgggtgcgcg
     3721 gcctctacaa tttcgcggcg catccggacc tgacattttt tttcaaggcg caactggaag
     3781 tttcgttgca gcgcattttg gacggacgtc cgcaattgaa atatttcgag gcaggcatgg
     3841 acatgcggct ttcgaccgat ccttacgaaa gctttcgcat cttccaggga cgcatcctcg
     3901 aacaatacct gggcatgagc acggagttca atttttcggt catcgatgcc aaccagccga
     3961 ttgaagcgca acaatcggtc gtgcgcgaat tgatcgccgc caagattgac ctggcttctt
     4021 tcgctgccaa ccatgcgggg acattcaaat gaaacgcatc atctccaaac ccgtcatgcc
     4081 cttgagccag cgccgcgtca tggtgccgcg tccatccaaa cggcgcttct acggctatgg
     4141 cattcccaac gtggatttgc aacggctggc gggaaaattg atcgtcatcg aaggcgcgga
     4201 cggttcgggc cgctcgacgc agattgcgcg gttgcgcgac tggctggaag gctgcggcca
     4261 cgcgacggtg caggtcggtt tgaaacgctc gacgctcgtt agtgaggaat tggaacaggc
     4321 gcaaaacggg aacatcttga gccatacgac gttgagcctg ttttatgcga ccgattttgc
     4381 ggaccagttg gaaaacatca ttctcccggc gttgaaggcc gggttcatgg tgctggccga
     4441 ccggtatatt tatacgttga tggcgcgcga cctggtgcgc gggatggaag aggcgtggtt
     4501 gaaaaatctt tacggcatcg cgctggcgcc ggacgcggtg ttttacctga acgtgtcgcc
     4561 cgagcaactg gtggaacgca attttgcgaa gaattacgct ctggattatt gggaaagcgg
     4621 gatggacctg gggctttcgc gcgaaatgtt cgacagtttc ctgaagtatc aatccttgat
     4681 ggcgcaggaa ttcaaacggt tgcagtccat ttacggattc agcatcgtgg atggcaaccg
     4741 gacgccggac gaaatcaacg tggaattgca gaaaaagatt gaagcggtgg tgacgggtaa
     4801 gtaaacagcc aatgacgaaa tacccaaata aatcccaatc accaacatcc aagctccagt
     4861 gaagttccaa attccaagct caaaattgcc tgatgaatct ttgatcactc cggttgcaat
     4921 cgccggaagt ttttggagat ttcgtgcttg ggtttttttg gggtatttag acagtgactt
     4981 gttgaacccg caaatgattt aaaaacatgg ccgaagcaaa caacaaaggc gagtacctgg
     5041 tgggtgtgga cctgggcggc accaagattt tggcgggcgt gttcaccacg tcgttgaaat
     5101 gcgtgggccg ctcgaagatg agcaccaagg ccgaacgcgg cccggaggcc gtcatcgaac
     5161 gcatcgcgca ttgtgttcag gacgccgtgg atgaatgcga tttgagcctc aaacaggtgc
     5221 gcggcattgg cattggcgcg cccggttcgg tggacccaga cagcggcaag gtgatgttcg
     5281 cagggaatct tggctggaag gacatgccgt tgaaacgcga attggaaagc catattgggc
     5341 tgccggtttt tttgcagaat gattgcaacg tctgcgccct cggcgtgcat gaggtcgagt
     5401 tggaaggcaa gccgaagaac atgatcggca tttttctcgg ca
//
LOCUS       DASZMM010000033         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_9912, whole genome shotgun sequence.
ACCESSION   DASZMM010000033 DASZMM010000000
VERSION     DASZMM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9912"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VG754_00790"
     CDS             complement(<1..596)
                     /locus_tag="VG754_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWF17768.1"
                     /translation="MSSSAAIKSPDALSESQKAALVNLLGDDDPAIYQLVRDKILSCG
                     DAATEWLRPHTLSSEPALRRRAQEIVQHFARRAADDRFLAFCLTEGNDLNLEQGAWLL
                     AQTQYPDINPDAYQALFDSYAGELRERVDSRADADTLLGAFNQYVFDVLRFKGNEQNY
                     YDPENSYLNRVVDRRLGNPINLSLVYLLLARRLKLPVTG"
     gene            832..1281
                     /locus_tag="VG754_00795"
     CDS             832..1281
                     /locus_tag="VG754_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWF17769.1"
                     /translation="MHVSGTVDLLLNEKSPTLWSVSPGATVFDAIRLMSEKNIGALLV
                     MEGERLVGMMSERDYTRKVILKGKSSKTTAVREIISTPVISVDPCTTVEECMRLMTEH
                     RIRHLPVLNRGMLVGIVSIGDLVNWTISAQHVAISQMEDYISGKYPG"
     gene            complement(1315..2454)
                     /locus_tag="VG754_00800"
     CDS             complement(1315..2454)
                     /locus_tag="VG754_00800"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWF17770.1"
                     /translation="MKPTIRISAADRTLLTGLLVLNLSLNSNARELTRNSVSQPARSN
                     LLQEEFTPGFLAERLGIAQKSTTLLGASVKEYSGKKIGKLEDVVVDLHSGRVLCALVS
                     AGGAVTIAIPSKVFFETDMKRAQLAINDVKMDAVPRVNPDTAGLVSSLPASYSYFNQH
                     MFWDAGAQIEARKCSELVGMNVKNNAGESLGRIVNLMVDLPTARVVVVVVSFAGKGTD
                     LYAVPPQAFSAAADNSGLVLSMDSAKVSSLAQSDGFFWSNMADANWVGNAYHAFGERP
                     DFDSAANTASATTTAVENVSASAQPLSSAPAKTDSELSQDILAAIMLDNISNLTIYKH
                     AQIKPANGQVTLRGWVKNENQRSKLVSIASGVAGAANVHDEIQIK"
     gene            complement(2652..>4025)
                     /gene="ilvD"
                     /locus_tag="VG754_00805"
     CDS             complement(2652..>4025)
                     /gene="ilvD"
                     /locus_tag="VG754_00805"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWF17771.1"
                     /translation="TVVGCEGMDGFVAIGGCDKNMPGSMIAIARLNRPAVFVYGGTIL
                     PGCLTGDTRKLDIVSVFEAVGAHANKKISDNELQAIESCAIPGPGSCGGMYTANTMAS
                     AIEALGMSLPNSSAQAATSPAKKKDCLQAGAAVVNLMKLGLKPLDIMTRKAFENAITV
                     VIALGGSTNAVLHLLAMANAAKVKLALDDFTRIGKRVPVLADLKPSGKHLMSELVAIG
                     GIRPMMKTLLDAGLLHGDCRTVTGKTVREDLEGVAPYPPSQTIIRPLSEPIKKDSHLV
                     VLYGNLSPEGSVAKISGKEGLRFEGRARVFNSEEQALKKILDGGIQKGDVVVIRYEGP
                     KGGPGMREMLAPTSAIMGKGLGKEVALITDGRFSGGTHGFVVGHITPEAYVGGPLALV
                     KERDRIHIDAEKRELILEISTKEMQARRKNWKAPKPRYTRGVLAKYAHLVTSATLGAV
                     TDQTLKL"
BASE COUNT          862 a         1208 c         1072 g          883 t
ORIGIN      
        1 ccagtcaccg gcagtttcaa ccggcgcgcc agcaaaagat aaacaaggct gagattgatg
       61 gggttgccca ggcggcggtc cacaacgcgg ttcagataac tgttctccgg gtcgtaataa
      121 ttttgttcat tgcctttgaa ccgcagcacg tcaaagacgt attgattgaa tgctcccaaa
      181 agagtgtcgg catcggcgcg cgaatcaacc cgttcgcgca attcacccgc ataactgtcg
      241 aacaacgcct ggtaagcgtc cgggttgatg tcgggatatt gggtttgggc gagcagccat
      301 gcgccttgtt ccaaattgag gtcgttgcct tccgtgaggc agaaggcaag aaagcggtcg
      361 tccgccgcgc gacgcgcaaa atgctggacg atttcctgcg cccgcctccg caatgccggt
      421 tcactgctca acgtatgggg acgcagccat tccgtggcgg catcgccaca tgagaggatt
      481 ttgtcgcgca ccaactggta aatggccggg tcatcatcgc caagaagatt cacgagggcc
      541 gctttctggc tttcggaaag cgcgtcgggc gatttgatgg cggcggagga actcatcgct
      601 gactaaaaaa gcatggcgca attaaacgga aattacaatc ctaaaagaga actgaatttt
      661 gaagaaactt tctttacaat catcatcgaa tgcgcttagc tttacccaat ccgaagcaac
      721 cagctcacat gagaacaatc gagcagcttc aatccgtctt ggcctttgtg gccgacggaa
      781 aagttgtatt cgcaaagggc agttcaatcc tcaaacccca ggaaaattct tatgcacgtc
      841 tccggcacag ttgatctttt gctgaacgaa aaatcaccga cgctctggtc agtctcgccg
      901 ggcgcaacgg ttttcgacgc catccgtctc atgtccgaga agaacatcgg cgcgttgctc
      961 gtgatggagg gagagcggtt ggtcgggatg atgtcggaac gcgattacac gcgcaaggtc
     1021 atcctgaaag ggaaatcttc gaagaccact gccgtgcgcg aaatcatttc cacgccggtc
     1081 atctcggtgg atccgtgcac cacagtcgag gaatgcatga ggctcatgac cgaacatcgg
     1141 atacgccacc tgccggtgct gaatcgcgga atgctggtcg gcatcgtttc catcggtgat
     1201 ttggtgaact ggaccatctc ggcccagcac gtcgcgattt cccagatgga agattatatc
     1261 agcggcaaat acccgggttg atccccagta ggttcggaat ttctcccggc acggctattt
     1321 gatctgaatt tcgtcgtgga cgttcgccgc gccggcaaca cccgacgcga tggacaccag
     1381 tttgctccgt tgattctcgt tcttcaccca gcccctgagc gtgacctggc cattggccgg
     1441 ctttatctgg gcatgtttgt aaatcgtgag attgctgatg ttgtcgagca tgatggccgc
     1501 aagtatgtcc tgcgaaagtt cactatcagt cttggccgga gcggaagaaa ggggttgtgc
     1561 cgaagcgctg acgttttcga ccgcagttgt cgtggcagat gcagtgttcg ccgctgaatc
     1621 aaagtccggg cgctctccga acgcgtgata agcgttgccg acccaattcg catccgccat
     1681 gttcgaccag aaaaatccgt cgctttgcgc gagcgacgaa accttcgccg agtccatgct
     1741 caacacgaga cctgaattgt cggctgcggc cgaaaacgcc tgcggaggca cggcgtataa
     1801 atccgttcct ttgcccgcga aggaaaccac caccaccacg acgcgcgccg tcggcaagtc
     1861 caccatcaag ttcacgatgc ggcccaagga ttcgcccgcg ttgttcttca cattcatccc
     1921 gacaagctcg ctgcattttc tcgcttcgat ttgcgcgccc gcgtcccaga acatgtgctg
     1981 gttgaaataa ctgtacgatg cgggcaatga actcacgagc ccggcggtat ccgggttgac
     2041 acgcggaaca gcgtccatct tcacatcatt gatggcgagc tgcgctcttt tcatatccgt
     2101 ctcgaaaaaa acctttgatg ggattgcgat tgtcaccgcg ccgcccgccg aaacgagcgc
     2161 gcataaaacg cggcccgaat gcaaatcaac caccacgtcc tccagcttgc cgattttttt
     2221 gccggaatat tccttcaccg aagcgcccag caacgtggtg gatttttgcg cgatgccgag
     2281 ccgttccgcg agaaagcccg gcgtaaattc ctcctgcaaa agattgctgc gggcgggttg
     2341 cgaaacgctg ttgcgggtga gttcacgcgc attgctgttc agcgagaggt tcagcacaag
     2401 caaaccggtc aacaacgtcc ggtccgcggc ggagatgcgt atcgtcggtt tcaaggaagc
     2461 ctttctaaaa tctgcgcgaa cgcagtgatt cgactgtgcc gaagaaggcc gcgaagcccg
     2521 gcttcggcgc aggtgtacgg cgtggaaaag tacacatgct ttcatactac aagtatgatt
     2581 tagcatcggc catgccgtta tcaaaattga gataaaggcc tgaaatcgcg ccttttaacg
     2641 cgttgctcct cctataattt caacgtctgg tccgtcaccg cgccgagggt cgcggacgtg
     2701 accaggtgcg cgtatttggc aagcacgccg cgcgtgtaac gcggcttcgg cgctttccaa
     2761 tttttgcgac gcgcctgcat ttcctttgtg gaaatctcca aaatcaattc gcgcttctcc
     2821 gcgtcgatgt ggatacgatc acgttccttc accaacgcga gcggcccgcc cacgtaagcc
     2881 tccggcgtga tgtgcccgac aacgaaaccg tgcgtgccgc ccgaaaaacg cccgtccgtg
     2941 atgagcgcga cttccttgcc caatcctttg cccatgatgg ccgaagtcgg cgcgagcatc
     3001 tcgcgcattc ccgggccgcc cttcggccct tcgtaacgga tcaccaccac gtcgcctttc
     3061 tggatgccgc cgtccaaaat tttcttaagc gcctgttcct cggaattgaa cacccgcgcc
     3121 cgtccttcaa aacgcaaacc ttccttgccg ctgatttttg cgactgaacc ttcgggcgac
     3181 aaattcccgt aaagcaccac cagatgactg tcctttttga tcggctcgct caatggccgg
     3241 atgatggtct ggctgggagg gtagggcgcc acgccttcca aatcttcgcg caccgtcttg
     3301 cccgtgaccg tccggcaatc gccgtgcaac aagcccgcgt ccaacaacgt tttcatcatc
     3361 ggccggatgc cgccgatcgc caccaactcg gacatcaaat gttttccgct cggcttcaaa
     3421 tcagcaagga ccggcacgcg tttgccgatg cgcgtgaaat cgtcgagcgc caatttcact
     3481 ttcgccgcgt tcgccattgc cagcagatgc aacaccgcgt ttgttgaacc gcccaacgcg
     3541 atcaccaccg tgattgcatt ttcaaaagcc ttgcgcgtca tgatgtcgag cggcttgagg
     3601 cccagcttca tcaagttcac caccgccgcg cccgcttgca ggcaatcctt cttcttcgcc
     3661 ggtgaagtcg ccgcctgcgc cgaactgttt ggcaaactca tgccgagcgc ttcgatcgcg
     3721 ctcgccatgg tgttcgccgt gtacatgccg ccgcaggaac cgggccccgg gatcgcgcaa
     3781 gattcaatcg cttgaagttc gttgtcgctg atttttttgt tcgcgtgcgc gccgaccgct
     3841 tcgaacaccg acacgatgtc cagcttgcgc gtgtcgccgg tgagacaacc cggcaaaatc
     3901 gtcccgccat aaacgaacac cgccgggcgg ttcaaccgcg cgatggcgat catcgagccc
     3961 ggcatgtttt tgtcgcagcc gccgatggcc acaaagccgt ccatcccttc gcaacccacc
     4021 accgt
//
LOCUS       DASZMM010000034         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3040148, whole genome shotgun sequence.
ACCESSION   DASZMM010000034 DASZMM010000000
VERSION     DASZMM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040148"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(484..1092)
                     /locus_tag="VG754_00810"
     CDS             complement(484..1092)
                     /locus_tag="VG754_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17772.1"
                     /translation="MKRTITTILATMLIANIAMASAELVKEKAKQQRDINNQQQGIAP
                     APSSPAPGVSAPSPSGPQPGISPAQQALVERLQNDLAIFKTGATVTPEQKSRIQDDLS
                     LLMKGSYKPSKATLAKLAEHLSAALAEKPLSPTYMTRLSRDINIVVNSAFLTPAQSQR
                     FVADAQLALKNGGVSAGTVQLIGNDLGSIINEIQKSKPKLYQ"
     gene            1283..1777
                     /locus_tag="VG754_00815"
     CDS             1283..1777
                     /locus_tag="VG754_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF17773.1"
                     /translation="MAAQTLDMHSVSPASLSDIPQLVGLLNLLFTQETDFAPDPARQE
                     RALRLILAQPEIGRIFCARADGRIIGMVSLLFSISTVKGGRVAWLEDMVVQPEWRSRG
                     VGESLLKHALERARAFNCSRVTLLTDETNTAGKRFYERAGFVRSQMVPFRLHLDFSAR
                     KSGG"
     gene            complement(1752..2732)
                     /locus_tag="VG754_00820"
     CDS             complement(1752..2732)
                     /locus_tag="VG754_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF17774.1"
                     /translation="MIEVRNLSKSFRTYKKQPGFGGALKGLFRRKYELTQAVKDVSFS
                     VAPGELVGFLGPNGAGKTTTLKMLAGLLYPTSGSATVLGYVPWERQDGYRRQFALLLG
                     QKNQLWWDLPARESLDLNAKIYGIPRDLFERTVAEMTELLAVKDKLNVMVRELSLGER
                     MKMELIASLLHQPKVLFLDEPTIGLDVISQKTVREFLREYNARRKTTILLTSHYMADI
                     QELCSRVIIIDHGAIYFDGSLSEIIDRFADFKIITIQCEPTDTAKANPPANLAHYGEV
                     IEQAAGSIKLKVKRDRVIPVCKALLDELPVSDIDIQEVPIEDVIRQIFAR"
     gene            complement(2909..>4495)
                     /locus_tag="VG754_00825"
     CDS             complement(2909..>4495)
                     /locus_tag="VG754_00825"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HWF17775.1"
                     /translation="LFGIGALLEEDDDGYCKIKSLTPGAPAARSKQIKPGDRIVAVAQ
                     GTNAPVDVVQMPLTKVVDQIRGLKGTEVRLTIIPADAPTSTRNVVSIKRDEIKLEDQQ
                     AKAKLVELTDDEGRTNRLGIIDLPSFYASFSVVGQRSRPEIKSATMDVDRLLTKLEKS
                     HVDGVILDLRHNGGGSLPEAIDLTGLFIKQGPVVQVKGSNPDEPVQVDEDTDPRVQYG
                     GPLVVLTSRFSASASEIVAGALQDYGRAVVVGGASTHGKGTVQSMSQLAPYVRGDYFT
                     NDPADFGALKYTTNKFYRVTGSSTQLKGVVPDIVLPSSYDYMETEEAREDNAMPWDEI
                     QSTKYDKLNLVQPYVAELEKRSEKRVAASKDFDYVREDIELVKKTMSDKSASLNERQQ
                     LKESAEAEASRKAREAEMKARKEPEVKTYDLTLKNGDVLMVEEKRASTNAPIAGVKSK
                     PGLGQESSPNPTAGADDTVSPDDIADANAQSATTDLPSPEEKAPMDEAEHILIDYISV
                     LHAHPDLTAEHQSASVLQSN"
BASE COUNT          897 a         1281 c         1244 g         1073 t
ORIGIN      
        1 gcggtcttcg cgagaaacca cgaagccaag ttccgggttc tgccgggaat ggttcaaagt
       61 ttaaggttta aggttgcgga tttcgcggac tttcgtgttt ttcgcgggca actcgcgtct
      121 cggttctctc atgcttggac gtttggcgga cttcgccgaa catggcccgt tctgaaggac
      181 ctgaaaactc acgttttcag ctacgttatt tccgcttgtt tcaccttgct tcggttccgc
      241 ttcaattcag atcaaccggg ataaaacggg ataaaaaaat aaatattttt tcatccggtt
      301 tggttgaaat tgagtgaaat ggcccaagtt gtaagtactt gagattccgc aaaatttcat
      361 aaagtttcac aaaattccgc cattttaacc cccggggggt gggctgacct gaacgttttt
      421 gggcttgatg cctccccgtc tcggacgggc ttttccaaat cagcactcca atagtccatc
      481 acttcattga tagagcttgg gtttgctttt ttggatctcg ttgatgatgc tgcccaaatc
      541 gttcccgatg agctggaccg tcccggcgct cacgccgcca ttcttaagcg caagttgcgc
      601 atctgccaca aatcgctgcg attgggcggg agtaagaaac gcggaattga cgacgatgtt
      661 gatgtctcgt gacaatcgcg tcatgtaagt gggcgaaagc ggtttttcgg cgagcgcagc
      721 cgacagatgc tcggcaagtt ttgcgagggt cgccttggaa ggtttgtagg agcctttcat
      781 gagcaaagag aggtcatcct gtatgcgcga cttttgctcc ggggtgacgg tcgcgccggt
      841 cttgaagatc gcgaggtcgt tctgcaaccg ctcaaccaat gcttgctgcg ccgggctgat
      901 gcccggttgc ggcccggaag gcgatggcgc ggaaacgccc ggcgcaggag aactgggcgc
      961 aggcgcgatg ccttgttgct ggttgttgat gtcgcgctgt tgcttggcct tttccttgac
     1021 caattcagcg ctggccatgg cgatgttcgc gatgagcatc gttgccagga ttgtggtgat
     1081 ggtccttttc ataacctata cagattttga atcgtcccat cttgcgcccg gcaatcctta
     1141 ttgtcaattc cgcaagagcg gcatgaaaag cgagattgca ggttacattt ggatggcata
     1201 ctgtacgaaa catatatgga ttcattgcga aaagggcctg acaagtcgcc ggaaccaagc
     1261 tgatttaaac tccaccacta ccatggcagc ccaaactttg gacatgcatt ctgtttcacc
     1321 ggcttcattg agcgacattc cgcagttggt cggattgttg aatctgctct tcacgcaaga
     1381 gaccgatttc gcgccggatc ccgcgcggca ggagcgcgcg ttgcggctta tccttgcgca
     1441 accggaaatc ggccgcattt tttgcgcgcg agcggacggt cgcatcatcg gcatggtgag
     1501 tctgcttttt agcatcagca ccgtcaaagg cggacgcgtc gcatggctgg aggacatggt
     1561 cgtgcaaccc gaatggcgca gtcgcggcgt gggcgaaagc ctgttgaagc atgcgcttga
     1621 acgcgcgcgc gcgttcaatt gttcgcgcgt cacgttgctc accgacgaaa cgaacaccgc
     1681 aggcaagcgc ttttacgaac gcgccggttt cgtgcgttcg caaatggttc cgttccggct
     1741 gcacttggat ttcagcgcgc gaaaatctgg cggatgacgt cctcgatcgg cacttcctga
     1801 atgtcaatgt cgctcacagg cagttcgtcc agcagcgcct tgcaaaccgg gatgacccgg
     1861 tcgcgcttca ccttgagctt gatgctgccg gccgcctgct cgatgacttc gccgtaatgc
     1921 gccaagttcg cgggaggatt cgcttttgcg gtgtcagttg gttcgcattg gatcgtgatg
     1981 atcttgaaat cggcgaaacg atcgatgatc tcgctcaagc ttccatcgaa ataaatcgcg
     2041 ccatggtcga tgatgatcac gcggctgcac aactcctgga tgtccgccat gtaatggctg
     2101 gtgagcagga tggtggtttt ccggcgggcg ttgtactcgc gcaaaaattc gcgcacggtt
     2161 ttttgcgaaa tgacatccaa gccgatggtc ggctcgtcca aaaaaagcac cttgggctgg
     2221 tgcaacagcg acgcgatgag ttccatcttc atccgttcgc caagcgaaag ctcgcggacc
     2281 atcacgttga gcttgtcctt gaccgcaagc aattccgtca tctcggcaac cgtgcgctcg
     2341 aacaagtcgc gcgggatgcc gtaaattttt gcgttaagat ccagggattc ccgcgccggc
     2401 aagtcccacc aaagctggtt tttttggccg agcaacaaag cgaactgccg ccggtaacca
     2461 tcctgccgct cccacggcac atagccgaga acggtcgcgc tgccgctggt cggataaagc
     2521 agtcccgcga gcatcttgag cgtcgttgtt ttgcccgcgc cattcggccc gagaaacccg
     2581 accagctcgc ccggcgcaac ggaaaaactc acgtccttga ccgcctgcgt cagttcatac
     2641 ttgcggcgaa aaagtccctt gagcgcaccg ccgaaacccg gctgcttctt gtaagtgcga
     2701 aaagatttgc tgaggttgcg gacttcgatt atcggcgcgg tgtcagacac ggatatcatt
     2761 aaaacacgct tcacgcgcga aatcaaagtg cattcgagag ggaaactgcg gagccgcaga
     2821 tttgaaattt atcgtggcag aatttccatg attcttctcc ctcacccccg tcggaggaga
     2881 gggttggggc gagggcgcga agcaacggtc aattcgattg cagcaccgag gcggattgat
     2941 gttccgcagt caaatcagga tgggcgtgca ggaccgaaat gtaatcaatc aaaatatgtt
     3001 cggcctcatc catgggcgct ttttcctcgg gcgaaggaag atccgtggtt gcagactgtg
     3061 cgttggcgtc cgcaatgtcg tccgggctga cggtgtcatc agcgcccgcg gttggattgg
     3121 gcgacgattc ctgaccgagg ccgggtttgg acttcacgcc ggcgatcggc gcattggtcg
     3181 aagctctttt ctcctcaacc atcaagacgt cgccattttt cagcgtgaga tcatacgtct
     3241 tgacctccgg ctccttgcgc gccttcattt cggcttcgcg ggcctttctg ctggcctcgg
     3301 cttcggcgct ttccttcaac tgttgccgct cattcaagga agcgctcttg tcggacatcg
     3361 ttttcttcac caactcgatg tcctcgcgca cataatcaaa atccttggat gccgccacgc
     3421 gtttctcgga acgcttctcc aattccgcga cgtacggttg caccagattc aacttatcgt
     3481 acttggtgct ttggatttca tcccacggca tcgcgttgtc ctcgcgcgct tcctccgtct
     3541 ccatgtaatc gtagctcgaa ggcaggacga tgtccggcac aacgcccttc aactgcgtcg
     3601 aagaaccggt gacgcgataa aatttgttcg tcgtgtattt gagcgcgccg aaatcagccg
     3661 ggtcgttcgt gaaataatcg ccgcgcacat aaggcgccaa ctggctcatg ctttgcaccg
     3721 ttcccttgcc gtgcgttgaa gctccgccca cgaccaccgc gcgcccataa tcctgcaacg
     3781 cgcccgccac gatctccgaa gccgaggcgc tgaaccggct cgtcagcacc accaatggac
     3841 cgccgtattg cacgcgcgga tcggtgtctt catccacctg cacgggttca tcgggattcg
     3901 aacctttcac ctgcacgacc ggaccctgct tgatgaaaag cccggtgagg tcaatcgcct
     3961 cgggcagcga accgccgccg ttgtggcgca aatcgagaat gacgccgtcc acatggcttt
     4021 tctccagctt ggtcagaagc cggtcaacat ccatcgtcgc gctcttgatt tccgggcgac
     4081 tgcgctgccc gaccaccgag aagctcgcgt aaaacgaagg cagatcgatg atgccaaggc
     4141 ggttcgtcct gccttcatcg tcggtgagtt caaccaactt cgccttcgcc tgctggtctt
     4201 ccagtttgat ttcgtcgcgc ttgatgctca cgacatttcg cgtggaagtc ggcgcgtctg
     4261 cgggaatgat ggtcaatcgc acttccgtgc ccttcagccc gcgaatctgg tccacaacct
     4321 tcgtcaacgg catttgcacg acgtcaacag gcgcattggt gccctgcgcg acggccacga
     4381 tgcggtcacc cggcttgatt tgcttgcttc gcgccgcggg agcgcccggc gtgaggctct
     4441 tgatcttgca ataaccatcg tcgtcctctt ccagcaacgc gccgatgccg aacag
//
LOCUS       DASZMM010000035         6272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2765790, whole genome shotgun sequence.
ACCESSION   DASZMM010000035 DASZMM010000000
VERSION     DASZMM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765790"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..802
                     /locus_tag="VG754_00830"
     CDS             152..802
                     /locus_tag="VG754_00830"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HWF17776.1"
                     /translation="MKTFPKIICLVPLLLVVAIAPLAAQTDNADTNSTPVTSPSAPAA
                     PKAPVPDNNAQPRVPRVPTAPQVHINPHGIYVDGSSGGFGFSGPSATEATVAIVAILS
                     PFACIVVVCGLIFESRRRRNELIHETLRTMIEKGVPIPPELISPPDRTRRRREKSDLR
                     SGLVMLALGVGLMILLAPMHVPVWPIGFIFVLMGGAFLLAWKLEKKDAANTEAAPK"
     gene            806..1357
                     /locus_tag="VG754_00835"
     CDS             806..1357
                     /locus_tag="VG754_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF17777.1"
                     /translation="MPFTDADLIARALSLEDQNAFGELVRRHQSPVRAFLTKMTHGDS
                     HLADDLAQETFLKAWKKLKTFRGEARFSTWLFGIAYNEFRGEARRRKELALEDIAEMP
                     EETEPMLSSGAHLRMDLTEALKALTSAERATIVLCCQNGLSHEEAAQVLECPLGTVKT
                     NVLRGREKLRRKLTLEYKNYALA"
     gene            1350..1679
                     /locus_tag="VG754_00840"
     CDS             1350..1679
                     /locus_tag="VG754_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17778.1"
                     /translation="MPESNDPLEALLHENDPYLEDGGFTARVVASLPPRRKSWLRPAI
                     LSGATLIGLALLIWLAPFAKDALSFDASGEISVNLNPQSVITLAVLLMGAASLGWGLF
                     AMLKWED"
     gene            1753..2862
                     /locus_tag="VG754_00845"
     CDS             1753..2862
                     /locus_tag="VG754_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF17779.1"
                     /translation="MKKFLSVLLLSLFFFSLQAQESKPPVRFAIIGLVHDHAGGFIPH
                     LKGREDVQLAGIVEPNAEVAARAVKHFNLDPNLIFPTLEALLARTNIQAVATFTATFD
                     HRRIAEECARLGIHVMMMEKPLAVNMKHARAIARAAKKGDIKIIVNYETTWYPGNQEA
                     YRMVHDQHAIGDLRKIVVHDGHPGPKEIGCSKTFTDWLTDPVLNGGGALPDFGCYGAD
                     LVTWLMDGERPESVLCVTQHIKPDVYPKVEDEATVLLTYPRAQGILQASWNWPYNRKD
                     IEIYGKTGYILIPKSTQMRVLKTGGPETEVTAPEIKGPDADSVSYLAAVVRGEIQPAG
                     LSSLGVNMIVTEILDAARKSAQTGRRVDLPERAGY"
     gene            3324..4517
                     /locus_tag="VG754_00850"
     CDS             3324..4517
                     /locus_tag="VG754_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWF17780.1"
                     /translation="MADGQSHLQNSTMRNRWSDSINRRVSAGLAGIVLLACAMGLGAA
                     QWSSEKANEWYSNRPWMAGCNYSPSTAINQLEMWQPETFDPATIDRELGWAEQLGFNT
                     VRVFLHNLLWTQDAEGFLGRMDKFLAIADKHHIGVIFVPLDAVWDPNPHLGKQHDPVP
                     HVHNSGWVQSPGAEILKSPARQDELKPYIQGVIGRFKNDPRVAAWDIFNEPDNPNVPA
                     YVKQELTNKADMALLLLKKSFVWAREVNPSQPLTAGVWERGRWGATDRLLPMEKFIFD
                     NSDIISFHNYDGLEELKRCVQDLRRFNRPILCTEYMARPHGSRFDPNFGYLKEQKVGA
                     INWGFVNGKTQTIYPWDSWTKTYTAEPPVWFHDIFRQDGTPFDVKEVAYIKAVTGKTA
                     EKASK"
     gene            4725..6227
                     /locus_tag="VG754_00855"
     CDS             4725..6227
                     /locus_tag="VG754_00855"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWF17781.1"
                     /translation="MSEPLLRAQNITKTFHGVKALDQAGLTLRSGEIHALMGENGAGK
                     STLIKVLTGVHVPDSGTIEFGGVAISPRSPREAEATGISTVYQEVNLIPTLSLADNIL
                     LGRQPRRFGFLRKREMKRRAKAALARLGLDLDVGRTAGSCSMAVQQMAAIARALDVQA
                     KVLILDEPTSSLDEREVEFLFKILRQLREQGMAILFVTHFLDQVYAVSDFITVLRNGK
                     FVGEYRASELPRLKLISAMLGREFEEMQHLREEKTEDANRTVFLRARGLGKRGLMQPF
                     DLEVASGEVLGLAGLLGSGRTEMAKLCFGIESPDSGELSIGGQQIQIRSVRQAVRHGL
                     AFCSEDRKSEGLVPHLSVRENLILAMQASRGPLRLLPRKEQEQLTEHYINALKIKTPD
                     AETPIQNLSGGNQQKVLLARWLALQPKLIILDEPTRGIDVGAKAEIEKLVHTLRGRGL
                     AVLFISSDMEELVRVCQRLAVLRDRKKVGELSGANVQVNQIMNTIAQPHE"
BASE COUNT         1415 a         1692 c         1802 g         1363 t
ORIGIN      
        1 tttcgaaggt aaaaaattcc tgaatccaaa atcgcgcttc gtcgcatccc agttgttgaa
       61 tgacggacac agacagctcg caaaatactt gaatccaaaa aagctgctca tcgcctctca
      121 tttgtgagac aataaaccaa catcgctcaa tatgaaaacg ttcccaaaaa taatctgcct
      181 tgttccgttg ctgctggtgg ttgcgattgc gccgctcgcc gcgcaaaccg acaacgccga
      241 taccaactcc actcccgtca catcaccttc tgcgcccgcc gcgcccaaag cgcctgttcc
      301 ggacaacaac gcccaaccac gagttccccg agtgccgacc gcgccgcagg tccatatcaa
      361 tccgcacggc atttacgtgg atggctcatc gggcggattt ggcttcagcg gtccgagcgc
      421 aacggaagcc acagtagcca tagtggccat tctctcccca ttcgcatgca tcgtcgttgt
      481 gtgcggattg atttttgaat cgcgccgccg gcgcaacgaa ctgatccatg aaaccctccg
      541 cacgatgatt gaaaaaggcg tgcccattcc gccggaattg atttcaccgc ccgaccggac
      601 ccgccgccgc agggagaaaa gcgatttgcg aagcggcttg gtgatgctgg cgttgggggt
      661 tggattgatg atcttgctgg cgccaatgca cgtgccggta tggccaatcg ggttcatttt
      721 tgtcctgatg ggcggggcgt tcctgctcgc gtggaagctg gagaaaaagg acgccgccaa
      781 cacagaagcc gcgcccaaat aacgtgtgcc tttcaccgac gccgatctca tcgcgcgcgc
      841 tctctcgctg gaggaccaaa atgccttcgg cgagttggtg cgccgccatc aatcgccggt
      901 gcgcgcgttt ctcacaaaga tgacacacgg cgattcgcac ctcgccgatg acctcgcgca
      961 ggaaacattt ttgaaggcat ggaaaaaact gaaaacattt cggggcgaag cgcggttttc
     1021 gacgtggctt ttcggcattg cctacaacga atttcgcggt gaagcccgac ggcgcaagga
     1081 actggcgctg gaagacatcg cagaaatgcc ggaagaaaca gagccgatgc tctcgtcagg
     1141 cgctcatctg cggatggatt tgaccgaggc gttgaaggca ttgacatccg ccgagcgcgc
     1201 cacaatcgtg ctttgctgcc agaacggact gtcgcatgaa gaagccgcgc aagtcttgga
     1261 atgcccgctg ggtacggtca aaacgaacgt gttgcgtggc cgcgaaaaat taagacgcaa
     1321 acttactttg gagtataaaa attatgccct tgcctgaaag caatgatccg ttggaagcgc
     1381 tgctgcacga gaatgatccc tacctcgagg acggcggttt caccgcgcgc gtggtggcgt
     1441 cgcttccgcc acgacgaaaa tcatggctgc gtcccgcgat cttgtccggc gcaacgctaa
     1501 tcggactggc gttgctcatc tggctggcgc catttgccaa ggacgccctg agtttcgatg
     1561 catccggcga aatttcagtg aatctgaatc cgcaatccgt catcacgcta gcggtgttgc
     1621 tgatgggcgc ggcttcgctt ggctggggat tgttcgcgat gttgaaatgg gaagactgag
     1681 ctgttcacga tttgcgatat acgatttacg cgcattcttg ctcgcgggca caacctctgc
     1741 catgctgcga ccatgaaaaa gtttctgtcc gttttgttgc tctccctttt tttcttttca
     1801 cttcaggccc aggaatccaa accgccggtg cgctttgcca tcatcgggct ggtgcatgat
     1861 catgcgggcg gttttattcc gcacctcaag ggacgcgaag acgtccaact cgcgggcatc
     1921 gtcgaaccaa acgccgaagt cgccgcgcgc gcggtcaaac atttcaatct cgatcccaac
     1981 ctcatttttc ccacgcttga agcgctgctt gcccgcacga acatccaggc ggtcgcgaca
     2041 ttcaccgcca cgtttgacca tcgccgcatc gctgaagaat gcgcccgcct tggcatccat
     2101 gtgatgatga tggaaaaacc tctcgccgtg aacatgaagc acgcgcgcgc catcgccagg
     2161 gccgcaaaaa aaggagacat caagatcatc gtcaattacg aaaccacctg gtatcccggc
     2221 aaccaggaag cttaccggat ggttcacgac caacacgcaa tcggcgattt gcgaaaaatc
     2281 gtggttcatg acggccatcc cggccccaag gaaatcggct gttcaaaaac attcaccgac
     2341 tggcttaccg acccggtgct gaacggcggc ggcgcgttgc cggattttgg ctgctacggc
     2401 gcggacctcg tgacctggct catggacggc gaacgtcccg aatccgtcct ctgcgtcacg
     2461 caacacatca agcccgatgt ttatcccaag gtggaagatg aagccacggt gttgctcacc
     2521 tatcccaggg cgcaaggcat tctccaagcc tcatggaact ggccatacaa ccgcaaggac
     2581 atcgagatct acggcaagac cggttacatc ctcattccca agagcactca gatgcgtgtc
     2641 ctcaaaaccg gcggtccaga aactgaagtc accgccccgg aaatcaaggg gccggacgct
     2701 gattcggtca gttatcttgc cgcggtcgtt cgcggtgaaa tccaaccggc cggtctttcc
     2761 tcgcttggcg taaacatgat tgtgaccgaa atcctcgatg ccgcgcgcaa atccgcgcag
     2821 acaggacggc gggtggattt gcccgaaagg gcggggtatt gaaggaaaga aacattgaac
     2881 gctgaacttc caacgctgaa catcgaatgt ttcccgactt tgtactcatt ggatgttcaa
     2941 cgttcgatgt gctttgtaga aaacatgtct ggttcatgga aaagaatgga accgagatag
     3001 gagtttgggg ggcgcatctc tgcttttgcg caacgggtat ttgaacagtt cctttcccct
     3061 ctccccggcc ctttcccatc gggagaggga gcattgtctt ccatcgtggt tgcaattcaa
     3121 tgcactcgag tgaaccaagc agcggacaaa cggttcaccc ctcttggctg gaatcatgcc
     3181 ggtgaaaaag gggagcgcac gcctccggcg tgcagtgttc ggcgtctcgc cgaacacttc
     3241 gtaatactga ttttctgccc gctcgaaaat gcgattgtgc tttgccgcgc ctgctgctag
     3301 gaaatctgtc tgaattccat gtcatggctg acggtcaaag tcatttgcag aactcgacta
     3361 tgcgaaaccg atggtccgat tcgataaacc ggcgggtgtc cgcgggtttg gccggtattg
     3421 tcttactggc ttgcgcgatg ggtttgggcg cggcgcaatg gtcgtcggag aaggccaacg
     3481 aatggtacag caaccggcca tggatggcgg ggtgcaatta ttcgccgagc accgccatca
     3541 accagttgga aatgtggcag ccggaaactt tcgatccggc cacgattgac cgcgaacttg
     3601 gctgggcgga gcaacttggc tttaacacgg tgcgggtgtt cctgcataac ttgctttgga
     3661 cgcaggatgc ggagggtttt ctcggtcgca tggacaagtt tctggcgatt gcggacaagc
     3721 atcatatcgg cgtgattttt gtgccgctgg acgcggtttg ggacccgaat ccgcatctcg
     3781 gcaagcagca cgatccggtt cctcatgtgc ataattccgg gtgggtgcaa agtccgggcg
     3841 cggaaatcct caagagcccg gcgcgtcagg atgaattgaa gccatacatc cagggcgtga
     3901 tcgggcgttt caagaatgac ccgcgcgtgg cggcgtggga cattttcaac gagccggaca
     3961 atcccaacgt gccagcctat gtgaaacaag agctgaccaa caaggccgac atggctttgc
     4021 tgctgttgaa aaaatctttt gtttgggcgc gagaagtgaa tccgtcgcaa ccgctcacgg
     4081 cgggagtttg ggaacgggga cgttggggcg caacggaccg gttgctgccg atggagaagt
     4141 tcatctttga taattcggac atcatcagct tccacaatta tgacgggctt gaagaattga
     4201 aacggtgcgt gcaagatttg cgccgtttta accggcccat cctctgcacc gaatacatgg
     4261 cgcgtccgca cgggagcagg ttcgatccca acttcggtta tctgaaggaa caaaaggtcg
     4321 gcgcgattaa ttggggtttt gtgaacggga agacgcagac gatttatccg tgggattcat
     4381 ggacgaaaac ttacaccgcg gagccgccgg tttggttcca tgacattttc cggcaggacg
     4441 gcacaccatt cgatgtgaag gaagttgctt atatcaaggc ggtcactgga aagacggcgg
     4501 aaaaagcgag caaatgaagc gactgaattt tgaaaatcgc caactctgca tcgtagcgca
     4561 gagtttcact ctgctgtatc gcagagttct actctgcaag ggccgcaaaa aaaggagcgc
     4621 ttttgaaaat ctcccgcgca attccatttc ctcagtgatg ccgattgcaa gccggcgata
     4681 cagcagatta caaatctgcg ctacattgtt tcaccactga actcatgagc gaaccattgc
     4741 ttcgagcgca gaacatcacg aaaactttcc atggcgtgaa ggcgctcgac caagccgggc
     4801 tcacgttgcg ttcgggagag atccacgcgt tgatgggcga gaacggcgcg ggaaaatcca
     4861 cgctcatcaa ggtgcttaca ggcgttcatg tgccggacag cgggacgatt gaatttggcg
     4921 gagttgccat cagcccgcgt tcgccgcgcg aagcggaagc aacgggcatc agcacggtct
     4981 atcaggaggt gaacctgatt ccgacacttt cgctcgcgga caatattttg ctcgggagac
     5041 aaccgcggcg ttttggtttt ttacgcaaac gggagatgaa acgccgcgcg aaagcggcct
     5101 tggcgcggtt ggggctggac ttggacgtgg gacgcacggc cggttcctgt tccatggcgg
     5161 tgcagcagat ggcggcgatt gcgcgcgcgc tggatgtgca ggcaaaagtt ttgatcttgg
     5221 acgagccgac ttccagcctc gatgaacgcg aggtggaatt tttgttcaaa attttgcggc
     5281 aactacgcga acaaggaatg gcgattcttt tcgtcacaca ttttttggac caggtctatg
     5341 cggtctcgga ttttatcacg gtgttgcgca acggaaaatt tgttggcgaa tatcgcgcca
     5401 gcgaattgcc gcgactgaaa ctgatcagcg cgatgctcgg acgggaattc gaggagatgc
     5461 aacatttgcg tgaagagaag actgaggacg cgaaccggac cgtcttcctg cgggcgcgcg
     5521 ggttgggcaa acgcgggctg atgcagcctt ttgatttgga ggttgcctcg ggcgaagtgc
     5581 tgggcttggc gggtttgctc ggttcgggcc gcacggagat ggctaaatta tgtttcggca
     5641 tcgagtcacc ggactcaggc gagctttcga ttggcggaca acaaatccag attcgttctg
     5701 tgcggcaggc cgttcgtcat ggcttggcgt tttgttcgga agaccggaaa agcgagggtt
     5761 tggtgccgca tttgtcggtg cgggaaaatt tgatccttgc gatgcaggcg agccgcgggc
     5821 cgttgcggtt gctgccgcga aaggagcagg agcaattgac ggagcattac atcaatgcgc
     5881 tgaaaatcaa aacgccggac gccgaaacgc ccatccaaaa tctttccggc ggcaaccagc
     5941 aaaaagtttt gcttgcgcgc tggctggcgt tgcaacccaa gctcatcatc ctcgacgagc
     6001 cgacgcgcgg cattgatgtc ggcgcgaaag ccgagattga aaaactggtc cacacgttgc
     6061 gcggacgcgg ccttgcggtg ttgtttattt catcggatat ggaagagctt gtcagggttt
     6121 gtcagcggtt ggccgtgctg cgcgaccgca agaaagtggg cgaactttcc ggcgcgaacg
     6181 ttcaggtgaa ccagatcatg aacacgattg cccagccgca tgaataattc ccccgccccg
     6241 ttgaaattaa aatcatggtt ccagggcgcg tc
//
LOCUS       DASZMM010000036         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_287298, whole genome shotgun sequence.
ACCESSION   DASZMM010000036 DASZMM010000000
VERSION     DASZMM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287298"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VG754_00860"
     CDS             complement(<1..483)
                     /locus_tag="VG754_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF17782.1"
                     /translation="MKALVTGGAGFIGSHLAEALCLRGAKVIVVDNLSLGKTANLDWK
                     RNGDELEFVRADISDEPLMKQLVRDCDWIFHEAAMPSVPISVAEPVETNTQNLDATLN
                     LLVLARDAGVKRFLFASSSAIYGDSDASAKNECLPPQPLSPYALQKYGAEKYGQLFHQ
                     L"
     gene            complement(586..2775)
                     /locus_tag="VG754_00865"
     CDS             complement(586..2775)
                     /locus_tag="VG754_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF17783.1"
                     /translation="MVSFFAKLLRLCRPYRQRLALGILMGLLGGLMEPLLMVCVKVAV
                     DVAFPGATVMSAGSPMFSRESINDLPALTARLQSHKDEVSKYLYSRFSLLSQEKITAY
                     SPTNSAPDLLPETLSAEFNLALLDPALYDERRFADVKLSKAAQQLLAGNPSDVARTKL
                     NRMLLESAYPTELTSMKLSVLDKYPWLQQRLAGLTKWLPTSRGSTTSLSITLAILSIP
                     MMMLLRGLVSYLNVYFLQWVAVRAIADLRSKLFAHLIQMPLGMLTKMSTGELISRVMG
                     DTGALQNAISGSLVVLIKDPATLFFLIVFLLWQQPMLTLLSMVIFPICLVPIITYSRK
                     VRASSTAIQTQSAQLSRIIHESFTGNRIIKAYNLENNVVLRFRDATGNFITHYMRSTR
                     AGEMPGPLIEFFGAVGVACLFYYILILSHKRIGAGDFLFFVGCVFSMYRPIKSLSRLY
                     TQLEQANAASARVFKLLDTPTTLPEPADPLPLKAAGAEIKFDRVSFQYEADHARLVLQ
                     EFTLTVKAGQMVALVGHTGSGKTTVTNLLLRFYDPQKGSVRIGGIDIRKVMLSDLRHQ
                     IAVVTQDTLLFDESIRRNIELGRPGATEDEIIAAAKHAHAHEFIIEKPQGYATLVGEK
                     GVLISGGQKQRLAIARAILKNAPILILDEATSALDTQVERAVQAALEELMEGRTTICI
                     AHRLSTIQRADVIVVLDQGRIVEMGRHEELLGKNGVYRRLYELQFQV"
     gene            complement(2946..>4076)
                     /locus_tag="VG754_00870"
     CDS             complement(2946..>4076)
                     /locus_tag="VG754_00870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF17784.1"
                     /translation="SGLSGWDFAGLGCGGNLPSSLMKPPSRILMFKGHSAGIGDLLRS
                     SAAWRVLKNAYPNVELHLLLFTREPGYPSEQLIRDHHLLSSLAVIDKRTPNFPERRRF
                     WADLNRIAKETRPDMVIDFESGGLRTSQAALWLRLRWGARTIGINTLPGRNLFYSRAA
                     TSPKKFAQKRGLPWPMEYTDRDFVALSALGLERAGCPIELEETEAGRQARLALRREHG
                     LAESVPLLGLNIGCGTPDAVSKRPDLAMLSELMAGLQNEHGMQLVLTGAPFERAVNEE
                     FCAIHCKKSSTPIIDLAGKTNISGLTGIIKACEMFVSSDSGPYHMGVAMRVPTLAIFS
                     HDNRTHFHSHPWVRCCIASDAKSLPELRSAAEELLMWGTRAG"
BASE COUNT          930 a         1144 c         1138 g          864 t
ORIGIN      
        1 aagttgatga aataattgac cgtatttttc cgcgccatat ttttgaagcg cataaggtga
       61 aagtggctgt ggtggaagac attcattttt tgccgacgcg tcactgtcgc cgtaaatggc
      121 ggaagacgag gcgaagagaa agcgtttcac gccggcgtcg cgcgctagca cgaggaggtt
      181 taacgtggca tccaaatttt gcgtgttggt ctcgaccggt tcggcgaccg agatgggcac
      241 cgaaggcatg gcggcttcat gaaaaatcca atcgcaatcc ctcacgagtt gtttcatcaa
      301 tggctcgtcg gaaatatcag cccgcacaaa ctcgagttcg tcgccgttgc gtttccaatc
      361 caggttcgcg gtcttgccca gcgagagatt gtccacgacg atgactttcg cgccgcgcag
      421 gcaaagcgcc tcggccagat gcgacccgat gaaacctgcc ccgcctgtga ccaatgcctt
      481 catggatttg ttacatggct aaatcgttac atggttacaa agatcacatt aggtcgagca
      541 tgcaacgatg taacttttta accatgcaac cttccgaatt tcttttcata cctgaaattg
      601 cagttcatag agccgccggt aaacaccatt tttgccaagc aattcctcat gccggcccat
      661 ctcgacaata cgtccctggt ccagcaccac gatgacatcc gcgcgttgga tcgtggaaag
      721 ccggtgcgcg atgcaaattg tcgtgcggcc ctccatcaat tcttccaacg ccgcctgcac
      781 cgcgcgttcc acttgcgtgt cgagagcgct ggtcgcctcg tccaagatca agatcggcgc
      841 gttctttaaa atggcgcgcg cgatcgcaag gcgctgcttt tggccgccgg aaatcaagac
      901 gcccttttcc ccaaccagcg tggcgtaacc ctgcggtttt tcgatgatga actcatgcgc
      961 atgcgcgtgc ttggcggcgg caatgatttc atcctccgtc gcgccgggcc ggcccaactc
     1021 gatgttgcgg cggatgcttt catcaaaaag caaagtgtcc tgcgtcacca cggcgatttg
     1081 atgacgcaga tcgctcaaca tgactttgcg gatgtcaatg ccgccaatgc gcaccgagcc
     1141 tttttgggga tcgtaaaacc gaagcaaaag attggtcact gtggttttgc cggagccggt
     1201 gtggccgacc aacgcgacca tctggcccgc cttcaccgtg agcgtgaact cttgcaacac
     1261 gagccgcgcg tggtccgctt catattgaaa actgacgcgg tcgaatttga tttccgcccc
     1321 ggcggccttg agcggaagcg ggtcggccgg ttcgggcagt gtggtgggcg tgtcgagcag
     1381 cttgaaaacg cgcgcgctgg cggcgttggc ctgttccaac tgcgtgtaaa ggcggctgag
     1441 cgatttgatg ggccggtaca tcgaaaatac gcagccgacg aagaacagga aatcacccgc
     1501 gccgatccgt ttgtgggaaa gaatgagaat ataatagaac aggcacgcca cgccgacagc
     1561 gccaaaaaat tcaatcaacg gccctggcat ttccccggcg cgggtcgagc gcatataatg
     1621 agtgatgaag tttccggtgg cgtcgcgaaa acgcagcaca acattgttct ccaaattata
     1681 cgctttgatg atgcggttgc cggtgaacga ttcatggatg atgcgcgata actgggccga
     1741 ttgcgtctga atcgccgtgc ttgatgcgcg caccttgcgg ctgtaggtga tgatcggcac
     1801 gagacaaatc gggaagatca ccatcgaaag cagcgttaac atcggctgtt gccagaggag
     1861 gaaaacgatc aggaaaaaaa gcgtcgcggg atctttgatc agcaccacga gcgagccgct
     1921 gatggcattt tgcaacgctc ccgtgtcgcc catgacgcgc gaaatcagtt cgccggtgct
     1981 catcttggtg agcatcccga gcggcatttg gatgaggtgc gcaaaaagtt ttgagcgcag
     2041 atcggcgatc gcccggactg ccacccattg caggaaatat acgttgagat aagacaccag
     2101 cccgcgcaga agcatcatca tcgggatgga gaggatcgcc agcgtgatgc tcaaagatgt
     2161 ggtcgaaccg cgcgaagtgg gaagccattt cgtcaacccg gccaatcgct gttgcagcca
     2221 gggatatttg tcgaggacag aaagtttcat tgaagtcaac tcggtgggat aggcgctttc
     2281 caacagcata cgattgagct ttgtccgggc gacatcggag gggtttcccg cgagcaattg
     2341 ctgcgcagct ttggacaact tcacatccgc aaaccgtcgt tcgtcgtaaa gcgccgggtc
     2401 caacagcgcc agattgaact ctgccgacaa agtttccggc agcaaatcgg gcgcggaatt
     2461 tgttggcgag taggcggtga ttttttcctg cgacagcagt gaaaaacggc tatagagata
     2521 tttcgaaact tcgtccttgt gcgattgcaa ccttgccgtc agcgcgggca gatcattgat
     2581 gctttcgcgc gaaaacatcg gcgaaccggc agacatgacg gtcgcgccgg gaaaggccac
     2641 gtcaaccgcg actttcacgc aaaccatcag cagcggttcc atgaggccgc ccagcagacc
     2701 catcaaaatg ccaagcgcaa ggcgctgccg gtaaggccgg cagaggcgta gcagctttgc
     2761 aaagaaggaa accatgcgac taaagaattt gcatgagaac cccgccgcat ggctaggatt
     2821 tattctcgcg gaaagttgaa aaacatcgca cgcccaacgc tgaacgcgga acatcgaacg
     2881 cttcgcaggc tgaagccgct ttcaattcgg cgttcaaggt tcgatgtcgg atgttcagtg
     2941 ttcttttatc ccgctcgcgt cccccacatc agcaattctt cggctgcgct gcgcaattcc
     3001 ggcaatgact tcgcatcgga cgcgatgcag caacgcaccc acgggtgcga gtgaaaatgc
     3061 gtccggttgt cgtgactgaa gatcgccagc gtcggcaccc gcattgccac gcccatgtga
     3121 taaggcccgc tgtcgctcga aacgaacatt tcgcacgcct tgatgatgcc ggtcaatcca
     3181 ctgatgtttg ttttgccagc gaggtcgatg atgggcgtgg atgatttttt gcagtgaatc
     3241 gcgcaaaatt cttcgtttac tgcgcgctcg aatggcgctc ctgtcagcac caattgcatc
     3301 ccatgttcgt tttgcaatcc ggccattaat tcggaaagca tcgcaagatc gggacgtttg
     3361 ctcacggcat ccggcgtgcc gcagccgatg ttcaagccga gcaaagggac cgattccgcc
     3421 aatccatgtt cccgccgcaa cgccagtcgc gcctgacggc cagcttcggt ttcctccagt
     3481 tcaatcgggc aacccgcgcg ctccaaaccg agcgcgctca gcgccacaaa atcgcggtcg
     3541 gtatattcca tcggccacgg caaaccgcgt ttctgagcaa actttttcgg cgaagttgcg
     3601 gcgcgactat agaacagatt gcgccccgga agcgtgttga tgccgatggt tcgcgctccc
     3661 caacgcaatc ttaaccacag tgccgcctgc gacgtgcgca ggccgccgga ttcaaaatca
     3721 atcaccatgt ccggacgcgt ttcttttgcg atgcgattca agtcggccca aaatcggcga
     3781 cgttcgggga agttgggcgt ccgcttgtcg atgaccgcaa gactgctcaa caaatgatgg
     3841 tcccggatga gttgttccga cggataaccc ggttcgcggg tgaacagcag cagatgcaat
     3901 tccacatttg gataggcgtt cttcaacacg cgccacgccg ccgagctgcg caacagatcg
     3961 ccgatgcccg cgctgtggcc cttgaacatc aagatgcgcg atggcggttt catgagagac
     4021 gacggcaaat ttccgccgca accaagtccg gcgaaatccc agccagacag gccgct
//
LOCUS       DASZMM010000037        13084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4683267, whole genome shotgun sequence.
ACCESSION   DASZMM010000037 DASZMM010000000
VERSION     DASZMM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4683267"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1991
                     /locus_tag="VG754_00875"
     CDS             165..1991
                     /locus_tag="VG754_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWF17785.1"
                     /translation="MIPVIVIFLYLAVIATIGFVAFKKGRDNTEDFFLASRTIGPMVF
                     FLSLFATNMTAFAILGSSGLAYRRGIGIYGLMASSSGFVIPLTIFFIGTRLWALGKKF
                     GHQTQVQFFRDRWECDGIGTLIFALSAAMLVPYMIISIMGGGTVLQEISMAKGAHDAL
                     IPYWLGCAIVAVVVTVNVFFGGMRGTVWVNIFQTILFLCFGFVAVMVISHALPGGFGE
                     YMSKLGADPKTSSLLTREKMPPAEFWSYTLIPLSSIMFPHMAIMCFSARKMSAFKRTV
                     VIYPIAIMLIWLPCIFLGVLGVATLGKIADPDGVLLRMLAEYAPAWLAGLLGAGIISA
                     VMGSDAHQVLAMSTMFTKDIYVHYGGHQKYGEKAAVVFARGFILVVTIIAYLIALYLK
                     AKQGIFEIAIRFAFSGFAAMAPVMVAALFWKRSTKWGALASTLFVAITLVTFAFLQGS
                     PAPPPAMSSVKPPLAKATNNIEGITNAPALAKSDSPDTNKAVDVLKDTNAPMAEAKPK
                     IASGAPTAVAPKPKVDVIWQAGSHIILSRNPATGDVRFWNYTNTPPGGFMTVVPMVFG
                     SALCMVLFSLLTRPPSRATIEKYFSEPASASSPPVPSPANKV"
     gene            2027..3103
                     /locus_tag="VG754_00880"
     CDS             2027..3103
                     /locus_tag="VG754_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17786.1"
                     /translation="MNPDSATVKAAQEKLDAHVREIVQWHFSPDTGSPFWLDWAKKSG
                     WNPATEVKCFADLLRFPHFQDEWLRDLPNEVWVPKKYQGRPFSIFETGGTTGMPKQRI
                     GWDDYKVDYSEFSEKLSDAHFPRNHYWLMVGPTGPRRLRLAVEHLANVRGSSCYFVDL
                     DPRWVKKVIGNKQFDQARAYMDHVVDQAVTILKHRKVSGMFTTPKLLEALGEKVNLYD
                     AGIRGVFCGGTTMAPQYVRFIVEEVLENRIGFYPTYGNTLMGLAASVPLRPEDNYSIT
                     YYAPQPRAVLRIVDPNDTTQLKNYGEWGRVELTTLTKEFFMPRFLERDEAIRREPRAP
                     YAWDGVAEVRPFGAMEKNIVEGVY"
     gene            3168..3467
                     /locus_tag="VG754_00885"
     CDS             3168..3467
                     /locus_tag="VG754_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17787.1"
                     /translation="MSETIIKDAKFHIVAEGLAPDSKKRLSLSKALADASVTFNVYVN
                     TLGQIVLDPHKKIPAHEAWVFEDKEILASLKRGLKQAAQGKTRSLGSFAKHAREN"
     gene            3467..3787
                     /locus_tag="VG754_00890"
     CDS             3467..3787
                     /locus_tag="VG754_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17788.1"
                     /translation="MTRQLIFTDEAAGQLEQISKNPAKTGLYKQLQKTLGLMETNLRH
                     PSLNTHEYSSLKGPNGEKIFEAYVQNNTPGAYRVFFYYGPDIVLKGKRTPALTVLMIV
                     AHPK"
     gene            3810..4592
                     /locus_tag="VG754_00895"
     CDS             3810..4592
                     /locus_tag="VG754_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17789.1"
                     /translation="MNSIPKSPGSETPLTKFRWPMAVVLVAFLFVGAWVISIKSCARA
                     APDTVHAVGTEVSNLLKNFDQQHITRTFQESLPQLNSEPGGRLEIAALTLNENLSESN
                     ALTTAWGHLDLGSTVTEIKVPAIYRYYVRLHDPWQLEVATNICVVHAPRIHPTLPVAI
                     DTSRMEKKSNVGWGRFNAGDQMDQLEKDLTPTLSLYAADARHIALVREECRKTVAQFI
                     RDWLLKEQQWRDDRFTAVKVVFPDEATTNAFTLPATLELKTQ"
     gene            4602..6032
                     /locus_tag="VG754_00900"
     CDS             4602..6032
                     /locus_tag="VG754_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWF17790.1"
                     /translation="MNLPHIPAMRRGRPYESLDQIKIVDHRTGAPLVSISQVNAGIIR
                     KDLARAAESRAALKKFTTAQLLDISAKAGELFLEGTLPLGDKGHAQSARQYLETLSAT
                     SGLPHVMVRRNMAKIHHALTHMRQILNGLTRGLDLGILDKGCGEQFGTPVSYYPTTHA
                     LGLVMPSNSPAVNSLWLPAIALKIPVIIKPGREEPWTPLRLIQAFIAAGCPAEAFGFY
                     PTDHEGAGEILKLCGRALIFGDKSTTDRYANNPAIQIHGPGFSKILIGADEIERWPEF
                     LDLMVASVSDNGGRSCINASAIVVPKYGAEIADALAQKLGPIAPLPATDENARLSGFA
                     NPKMAEYIDGAIEQDLKTPGATDVTAKYRNGPRKVEFEGGIYLRPTIVLCDSFAHPLA
                     NREFLCPYASIVEVPQSEMLKQIGPSLVVTAISKDPVFTEQLLESPLIERLNLGPIST
                     MKISWDQPHEGNMFEFLYKRRSIERA"
     gene            6049..7293
                     /locus_tag="VG754_00905"
     CDS             6049..7293
                     /locus_tag="VG754_00905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWF17791.1"
                     /translation="MSNTAQKPPEAHPSIPPFDHQPRTRIVFGVNNVERAGELARETG
                     AKNILLVTDPGIAAAGHAERVQKILETAGLKVILFAKAHENPTTRDVDECLAVAKAAA
                     SDTLVGLGGGSSMDTAKGCNFLLTNGGRMQDYWGTGKATKPMLPLIAIPTTAGTGSEC
                     QSFALIADEHTHQKMACGDPKAAARIAILDPALTVSQPTRVAACTGIDAITHAVETAV
                     TRRRNALSLMYSHEAFKLLVTSFPQVLRHPADLEARGRMLLGAALAGTAIENSMLGAV
                     HAAANPLTARYGIVHGQAVGLMLPAVVRFNGHNDAARQAYAELASAPEIACVSDGHGP
                     ALDALVARLETLLNLAQMPRSLADCGVKKSDLKTLATEAARQWTASFNPRPLTEADFV
                     QLYEVAFEPRGDGDGFGSMSVP"
     gene            7383..8063
                     /locus_tag="VG754_00910"
     CDS             7383..8063
                     /locus_tag="VG754_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17792.1"
                     /translation="MSETRTFSVCQGCGAWKETSANILEFFADYDMRFFIEQHATRCP
                     ATGAPAVLIAVSEDDANYRPLSTEKQWKPESYPGYRPPQKPRSIRHTTDIPERLLGIW
                     ATSHFGSHSDESLVFFPDGTGILEYWNVGFAGYQNFCWRLDGQERLLFGGANDEPTEN
                     VDSTDGAVSYFGTMRAYFELLLSTDIYGEQSEMLCLYRGDIQDEAQEFFRSKRSLENY
                     KIPDEFKP"
     gene            complement(8081..8875)
                     /locus_tag="VG754_00915"
     CDS             complement(8081..8875)
                     /locus_tag="VG754_00915"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HWF17793.1"
                     /translation="MIQYHQLLKLVLEEGKFKADRTGTGTYSVFGAQARFPLGDGFPA
                     LTTKKLHLKSIIYELLWFLRGDTNIKYLNEHGVTIWDEWADKDGNLGRVYGAQWCDWR
                     TADGRSINQIDEVIAQIKRNPDSRRHIVSAWSVGEIGQMVLPPCHALFQFFVADGELS
                     CQLYQRSADLFLGVPFNIASYALLTMMVAQVCDLKPGTFVHTFGDLHLYSNHLEQAKL
                     QLTREPRPLPQMKLNPAVKNVHDFKFEDFQLVGYDPHPAIKAPIAV"
     gene            complement(8884..11433)
                     /locus_tag="VG754_00920"
     CDS             complement(8884..11433)
                     /locus_tag="VG754_00920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF17794.1"
                     /translation="MVAPASSSVRVTVDGKFFRLGDKKFHLKGVSYGPFDPGNGEGTF
                     APPNQTAGDFAQIRELGANLIRVYYVPPKWFLELAAEYGLKVLVDIPWNKHLCFLDSE
                     KEKEAAREAVRQAAKNCAGHPAVFAYSIANEIPPDIVRWSGARAVAEFIDELVAEAKA
                     IDPDCLCTFTNYPPTEFLRPQTLDFFCFNVYLHNQQPFKNYLARLQMIADTKPLLLGE
                     FGIDSLREGEPRKCEILTWQIETTFRAGLAGTIVFSYTDDWWRGGLQIEDWMMGLTTR
                     DRQRKDSFRAVQKMFRLAPYFPLARHPRVSVVVASYNAERTLKACLDSLDRLNYPDYE
                     VILVDDGSTDSTSQIVAAHPQIRYFRHAENLGLSVARNTGIAAATGEIIAFTDADCRA
                     DTDWLYYLVGDLLDSEFVGIGGHNFLPPDDSSVAAAVLVSPGGPAHVMLTDRQAEHIP
                     GCNMAFYKWALEDVGAFDPIFHKAGDDVDLCWRLQQRGLKIGFSPGGFVWHYRRSTVR
                     AYLKQQHGYGEAEALLVRKHPEYFNIFGSSMWHGRIYTASKHGIIVRAPIVYHGVFGS
                     SWFQTLYTGQAMGLLSFFSSLEYNVVMTLPLLVLSSTFPWLLPVAITSLALSLGVCVA
                     AGYQAQLPRTKRRWWSRAVVSLLYFLQPIVRGWARYQGQLGLQLAPQTTKETLDSVAL
                     RGSGQSLNEVCFWAGAGVDRIEFVKGVLERLDKEGWPNKSDIGWSDYDIEVLGSRWTR
                     LQLTTVAEDCAGGKKILRCRLRARWSLQAKVAFWSALGFELLVIGVVGKWLPWLRLML
                     LSVAVFAWFLVWEKRNLQSLMVVVLDEVAKKWNMIKIPAKDGPGKTEKRGA"
     gene            complement(11466..11849)
                     /gene="rsfS"
                     /locus_tag="VG754_00925"
     CDS             complement(11466..11849)
                     /gene="rsfS"
                     /locus_tag="VG754_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWF17795.1"
                     /translation="MDSKKLALLCREFADNRKAENIVVMDVRELSSVTDYFVVASGTS
                     EPHLRAITEEITEKLRDEHDLRPRAVDGTLQTAWVVLDYFDVIVHVMRADVRDRYNLE
                     GLWGDAPQVKQRKKAKKAKVSEDLQ"
     gene            complement(11881..12468)
                     /gene="nadD"
                     /locus_tag="VG754_00930"
     CDS             complement(11881..12468)
                     /gene="nadD"
                     /locus_tag="VG754_00930"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWF17796.1"
                     /translation="MLLNLKKIGLYGGSFDPVHLGHLLVAQAACEEAGLERLFFIPAA
                     QSPFKPDAQPAPPAERLRLLRLALAGKSNYEIDEQEIKRGGVSYTIDTARDYAKRFPG
                     AELYYLIGADHVPSLGKWREAAELARLVEFVAIPRPGQEEPTFAPPFRGRTLKGFPLG
                     VSASQIRARVKAGLPIENLVAPAVAEAIRNYRLYL"
     gene            complement(12459..>13084)
                     /locus_tag="VG754_00935"
     CDS             complement(12459..>13084)
                     /locus_tag="VG754_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HWF17797.1"
                     /translation="LLSVTMTAGWEKIFYQETRPGFSKIGFLQIAQGLDKQLASLQVA
                     SIQAAVDAARNSGDSTAFQIAESAVRKNRIDHFNQMVDTAVVAFFLIMVATIFAISVW
                     EWLTLLLRKRAACLRESEAVWLPDYAMAESKPVNVLGVVALSCALARELSGEAHLERA
                     QQLAMQCKCAEGQVDLLGGKKASCAKSPEEIYLEVTQKRFDGINRCC"
BASE COUNT         2951 a         3787 c         3453 g         2893 t
ORIGIN      
        1 cttgtctttg gctttgttcc agtcggattg gcttatcacg cggcctattc aattttggcg
       61 gcgatcatga tggccattct cgtgaaattc gcctggccgg cgcatctcga acaatctgaa
      121 cctcacgatt catcgccgga ccagaaaaaa ggacaggacc actgatgatt ccggtcatcg
      181 tcatctttct ttatctcgcc gtcatcgcca ccatcggctt tgtcgcattc aagaaagggc
      241 gcgacaacac ggaggatttc tttctggcga gccgcaccat tggaccgatg gtttttttcc
      301 tgtcgctgtt tgccacgaac atgacggcgt tcgcgatcct cggcagttcc ggcctcgcct
      361 atcgtcgcgg catcgggatt tacggattaa tggcttcctc atccggcttc gtgattccgc
      421 tgacaatttt tttcatcggc acgcggttgt gggcgttggg aaaaaagttt ggccatcaga
      481 cccaggtgca atttttccgc gatcgctggg aatgtgatgg catcggcacg ctcatttttg
      541 cgctgagcgc ggcgatgctt gtgccctaca tgatcatcag catcatgggc ggcggaacgg
      601 ttttgcagga gatttcgatg gccaagggcg cgcacgatgc gcttattccc tattggctcg
      661 gctgtgcgat cgtggcagtt gtggtgacgg tgaacgtgtt cttcggcggg atgcgcggaa
      721 cggtctgggt gaacattttc cagacgattt tgtttctctg tttcggtttt gtcgctgtga
      781 tggtcatcag ccacgcgctg cccggtggtt tcggcgaata catgagcaag cttggcgcgg
      841 acccgaaaac cagttccttg ctcacgcgcg aaaaaatgcc gcccgcggaa ttttggagtt
      901 acacgctcat tccgctttcc tcgatcatgt tcccgcacat ggccatcatg tgtttttccg
      961 cgcgcaagat gagtgcgttc aaacgcacgg tggttattta tccgattgcc ataatgctga
     1021 tctggctgcc gtgcattttc ctcggcgtgc ttggcgttgc gaccctcggc aaaatcgcgg
     1081 accccgatgg agtgctgttg cggatgcttg cggaatacgc tcccgcgtgg ctcgccggtc
     1141 tattgggcgc aggcatcatt tctgccgtca tgggttcgga tgcccaccaa gtcctcgcga
     1201 tgagcaccat gttcacgaag gacatttatg tccactacgg cggccatcaa aaatatggcg
     1261 aaaaagccgc cgtcgttttc gcgcgcggat ttattttagt ggtgacgatc attgcgtacc
     1321 tcattgcgct ttatctcaaa gccaagcagg gcatttttga gatcgcaatt cggtttgcgt
     1381 tctccggttt tgcggcaatg gcgcctgtga tggtcgcggc gctgttttgg aagcgcagca
     1441 caaaatgggg tgcgctggct tcgacgctgt ttgttgccat cactttggtt accttcgcat
     1501 ttctgcaagg ctcacccgcg ccgccacctg cgatgtcttc tgtaaaacct ccgctcgcaa
     1561 aggccacaaa caatattgaa ggcataacga acgctccggc gttggcaaaa tccgacagtc
     1621 cggacactaa caaagccgtg gatgtcctca aggacacgaa tgcgccaatg gccgaagcga
     1681 aaccaaaaat agcatctggt gcgccgacag ccgttgcgcc caaacccaaa gtggatgtta
     1741 tctggcaggc tggcagtcac atcattcttt cacgcaatcc cgccactggc gatgttcgtt
     1801 tttggaatta cacgaacaca cctcccggcg gtttcatgac ggtcgtgccg atggtgtttg
     1861 gctcggccct gtgcatggtt cttttctcgt tgctgacacg tcctcccagc cgcgcgacga
     1921 ttgaaaaata tttttcagaa cccgccagcg ccagcagccc cccggttcca tcgccagcga
     1981 acaaggtttg aacgtccaat tactccaata ctccattttt gtctgaatga atcccgactc
     2041 cgcaaccgtc aaagccgcgc aggaaaaact cgatgcgcat gtgcgcgaaa tcgtccagtg
     2101 gcatttctcg ccggacaccg gctcgccctt ctggcttgat tgggcgaaaa aatccggctg
     2161 gaatcccgcc acggaagtga aatgtttcgc cgatctgctt cgctttccgc attttcaaga
     2221 cgaatggctg cgcgatttgc cgaacgaggt ctgggtgcca aaaaaatatc agggccgccc
     2281 gttcagcatc tttgagaccg gcggcacaac cgggatgccc aagcaacgca tcgggtggga
     2341 cgactacaag gtggattaca gcgagttttc cgaaaaactt tccgatgccc attttccacg
     2401 caaccattat tggctgatgg tcggtccgac cggcccgcgc cggctgcggc tggcagtcga
     2461 acatcttgcg aatgtgcgcg gcagttcgtg ttattttgtg gaccttgatc cgcgctgggt
     2521 gaagaaagtc atcggcaaca agcaattcga ccaggcgcgc gcttacatgg accacgtggt
     2581 ggaccaggcg gtgacaatct tgaagcatcg caaggtcagc gggatgttca ccacgcccaa
     2641 gctgctcgaa gcgctgggcg aaaaagtcaa tctgtacgat gcgggcattc gcggtgtgtt
     2701 ctgcggtggc acgaccatgg cgccgcaata tgtccggttc atcgtggagg aagttttgga
     2761 aaaccgcatc ggtttttatc ccacgtatgg caacacgctg atgggccttg ccgccagcgt
     2821 gccgctccgc cccgaggaca attattccat cacttattat gcgccgcagc cgcgggccgt
     2881 gttgcgcatt gttgacccca acgacacgac gcagttgaaa aattatggcg aatggggccg
     2941 cgtggaattg accaccttga cgaaggaatt cttcatgccc cggttcctcg aacgcgatga
     3001 agccatccgc cgcgaaccac gcgctcctta tgcctgggac ggcgtggctg aagttcgccc
     3061 gttcggcgcg atggaaaaga acatcgtgga aggcgtttat taaaaagtgg gaaagtgggg
     3121 aaaacccact tgcttgtcag agtgtttcat gttaaatatc gcatgatatg agtgaaacca
     3181 tcatcaaaga tgccaaattc cacattgtgg cggaagggct ggctcctgac agcaagaagc
     3241 gcctgtcttt gagcaaagcg ctcgcggatg cgagtgtcac cttcaacgtg tatgtgaaca
     3301 ccctcgggca gattgttctg gatccgcaca agaaaattcc agcgcatgaa gcatgggtgt
     3361 tcgaggacaa ggaaattctt gcttcgctga aacgcgggtt gaaacaggcg gcgcagggca
     3421 aaacccggag tttgggttca tttgcgaagc acgcgcggga aaattgatga cacgccaact
     3481 gattttcacc gacgaggcgg ccggacagtt ggaacaaata agcaaaaatc cggcaaaaac
     3541 aggcctgtat aaacagcttc aaaaaacttt gggattgatg gaaaccaatt tgcgtcatcc
     3601 ctcgttaaac acgcatgaat attcaagtct gaaagggccg aacggtgaaa aaatatttga
     3661 agcttatgtc caaaataata cgcccggcgc ctatcgcgtc tttttctatt atggtccgga
     3721 cattgtgctc aaaggaaaac gaaccccagc actgactgtc ttgatgattg tcgcccatcc
     3781 gaagtaatga cgggccgtta aagcaacgta tgaacagcat cccgaaaagc cccggttcgg
     3841 aaacaccgct gacgaaattt cgctggccga tggccgtggt gctggtcgca tttctgtttg
     3901 tgggtgcgtg ggtgatttcc atcaaaagct gcgcgcgagc ggcgccggac accgttcacg
     3961 ctgtcggcac ggaagtctca aatcttttga aaaattttga ccagcagcat atcacccgca
     4021 cttttcaaga gtcgctgccc cagctcaatt ccgaaccggg cggtcgcttg gaaatcgccg
     4081 cgctcacctt gaatgaaaat ttgtccgaat cgaacgctct caccaccgct tggggccatc
     4141 tcgatttggg cagcaccgtc accgaaatca aggtccccgc gatttatcgt tattatgtgc
     4201 gccttcatga tccatggcag ctcgaagtcg ctaccaacat ctgcgtcgtc catgcgccac
     4261 ggattcatcc gactcttccg gtggcaatcg acacctcgcg catggaaaag aaatccaacg
     4321 tcggctgggg ccgcttcaat gcaggcgatc aaatggacca gcttgaaaaa gacctcacgc
     4381 cgacactgag tctttacgcg gccgatgcgc gccacattgc attggtgcgt gaagaatgcc
     4441 gcaaaaccgt cgctcaattc atccgcgact ggctgctcaa ggaacaacaa tggcgcgacg
     4501 accgttttac agccgtcaaa gttgtcttcc ccgacgaagc cacgaccaat gccttcactt
     4561 tgcccgcgac gcttgaattg aagacgcaat aattttacgc catgaatctt ccccacattc
     4621 ccgcgatgcg ccgaggccgg ccttacgaaa gcctcgatca aatcaagatc gtggaccatc
     4681 gaaccggcgc gccgttggtt tccatcagcc aggtcaacgc cggcatcatc cgcaaggacc
     4741 ttgccagggc agccgaatcg cgcgcggctt taaaaaagtt caccactgcg caattgctcg
     4801 acatcagcgc gaaggcagga gaactgtttc tggaagggac gttgccgctc ggcgacaaag
     4861 gccatgctca aagcgcgcgc caatatctgg aaacactttc cgcaaccagc ggtctgccgc
     4921 atgtcatggt tcggcgcaac atggcaaaaa tccatcatgc gctcacccac atgcgccaga
     4981 ttttgaacgg gctcacgcgc ggcctcgatt tggggattct cgacaaaggc tgcggcgaac
     5041 aattcgggac accggtgagt tattatccga cgacccacgc gctcgggctt gtgatgccga
     5101 gcaattcgcc tgcggtgaat tcgctgtggc tgccggccat cgcgctgaaa atccccgtca
     5161 tcatcaagcc gggccgcgag gagccatgga caccgttgcg attgatccag gcgttcatcg
     5221 ccgcgggttg tccggcagag gcatttggat tttatcccac cgaccatgag ggcgcaggag
     5281 aaattttgaa attgtgcgga cgcgccttga tcttcggcga caaaagcacc acagaccgtt
     5341 acgccaacaa tccagccatc caaatccatg gcccgggttt tagtaaaatc ttgattggcg
     5401 cagacgaaat cgagcgctgg cctgaatttc tcgatttgat ggtcgcctcg gtttccgaca
     5461 atggcggacg cagttgcatc aacgcctctg ccatcgtcgt gccaaaatat ggagcggaaa
     5521 ttgccgacgc cctcgcgcag aagctcggcc ccattgcccc attgcccgcg actgacgaaa
     5581 acgcgcgtct atccggattt gccaatccaa aaatggccga atacattgat ggcgccatcg
     5641 agcaggattt gaaaacgccc ggcgcaacgg atgttactgc caaatatcgc aacggcccgc
     5701 gcaaggttga attcgaaggc ggcatttatc tgcggccaac cattgtgctc tgcgattcct
     5761 ttgcgcatcc cttggccaac cgcgaatttc tttgtcctta cgcaagcatt gtcgaagtgc
     5821 cgcaatcgga aatgttgaaa caaatcggcc cctcgctggt tgtgaccgcc atcagcaaag
     5881 accctgtgtt caccgaacaa ctgctggaat caccgctcat cgaacggctt aatctcggtc
     5941 ccatttccac gatgaaaatc tcctgggacc agccgcacga agggaacatg ttcgaatttt
     6001 tatataaacg ccgttcgata gagcgcgcgt gattaacaga aagctcccat gtcaaacaca
     6061 gcgcaaaaac cgccggaagc gcacccgtcg atcccgccat tcgaccacca accgcgcacc
     6121 cgtattgttt tcggcgtgaa caacgtcgaa cgcgccggcg aattggcgcg cgaaaccggc
     6181 gcaaaaaaca ttttgctcgt gaccgacccc ggcattgccg cggcgggtca tgccgagcga
     6241 gtgcaaaaaa ttcttgaaac agcaggattg aaagtcatcc tgtttgcaaa ggcacacgaa
     6301 aatcccacca cacgcgatgt ggatgaatgc ctggcagttg ccaaggcagc cgcaagcgat
     6361 accctcgtcg ggcttggcgg cggcagcagc atggacaccg ccaagggttg caattttctt
     6421 ctcacgaatg gcggccgcat gcaggattat tggggaaccg gcaaggcaac aaaaccgatg
     6481 ctgccgctga tcgccattcc aaccaccgcc ggcaccggca gcgaatgcca gtcgttcgcg
     6541 ttgattgcgg acgaacacac ccatcaaaaa atggcctgcg gcgatcccaa agccgccgcc
     6601 cgcatcgcca ttctcgatcc tgcgctcact gtttcacaac cgacgcgtgt cgccgcttgc
     6661 accgggattg acgccatcac ccatgcggtt gaaactgccg tgacgcgcag gcggaacgcg
     6721 ctttcattga tgtattcgca cgaggcgttc aaattgctcg tcaccagttt tccgcaagtc
     6781 ttgcggcatc ccgccgattt ggaagcgcgc gggcggatgc ttttgggcgc ggcattggcc
     6841 ggaactgcaa tcgagaacag catgctcggc gcggtccacg ctgcggcaaa tcctttgacg
     6901 gcgcgttatg gaatcgttca tggccaggca gtcggtctca tgctgcctgc cgtggtgcgg
     6961 tttaacggtc acaacgatgc cgcgcgtcaa gcttacgccg aacttgcctc tgcgccggaa
     7021 atcgcctgtg tcagcgacgg ccatggaccg gcattggatg ccttggttgc ccgtttggaa
     7081 actcttttga atcttgcgca aatgccgcgc tcgctggctg attgcggcgt gaaaaaatcc
     7141 gacctcaaaa cactcgccac cgaagctgcg cgccaatgga ccgccagctt caacccgcga
     7201 ccgctgaccg aagcagattt tgtccaactt tacgaggttg catttgaacc gcgcggagat
     7261 ggggatggtt ttggttcgat gtccgtccct tagagtgtat tcatgctctt gtggctttgc
     7321 atcttgatgc aaagcaagcg tctggtatgc tttatcaaaa ttgagggact tggagatctc
     7381 gcatgtctga aacccgcaca tttagcgttt gccaaggctg tggcgcatgg aaagaaacca
     7441 gtgcgaatat actcgagttt tttgccgact acgatatgcg ctttttcatt gagcagcatg
     7501 cgacccgatg cccggcgacc ggggcacccg cggttctgat tgctgtcagc gaagacgacg
     7561 cgaattatcg gcctctttca actgaaaaac aatggaagcc ggaaagctat cctggttacc
     7621 gaccgccaca aaagccgcgc tccatccgac atacaactga cattccggag cgtttacttg
     7681 gtatttgggc tacatcgcat tttggaagtc acagtgacga aagcttggtc tttttccccg
     7741 acggcactgg aattttagaa tactggaacg ttgggtttgc aggttatcaa aatttttgtt
     7801 ggcgtttgga tggccaagag cgtctgctgt tcggaggcgc aaatgatgaa cctaccgaaa
     7861 acgttgacag cactgacgga gcggtaagtt attttggcac tatgcgtgca tattttgagt
     7921 tgctcttgtc cacagacatt tatggcgaac agagcgagat gctttgtctt tatcgcgggg
     7981 acattcagga tgaagctcag gagtttttca gatccaaacg ctctttggag aattataaaa
     8041 ttcctgatga atttaagccg tgaaatcaat ttggccttga tcataccgct atcggcgcct
     8101 tgatggctgg atgcgggtca tagcccacga gttgaaaatc ttcgaatttg aaatcgtgga
     8161 cgtttttcac ggcgggattg agcttcattt gcggcagcgg acgcggttcg cgagtgagtt
     8221 gaagtttcgc ctgctctaga tggttgctgt agagatgcag gtcgccgaag gtgtgcacga
     8281 acgtgccggg cttcaaatcg caaacttgcg ccaccatcat cgtcagcaat gcgtatgacg
     8341 cgatgttgaa cggcacgcct aagaacaggt ccgcgctgcg ctggtaaagc tggcaactta
     8401 attcgccgtc agcgacaaaa aactgaaaca atgcgtggca cggcggcagc accatctgcc
     8461 cgatttcgcc gacactccat gcgctgacga tatgccggcg gctgtcggga tttcgtttga
     8521 tttgcgcgat gacttcatca atctgattga tggaacggcc atccgccgtt cgccaatcgc
     8581 accattgcgc gccatagacg cggccgaggt tgccgtcctt gtccgcccat tcgtcccaga
     8641 tcgtcacgcc atgctcgttc aaatatttga tgttcgtgtc accgcgcagg aaccagagca
     8701 gttcgtaaat gatggatttg aggtgcagct tcttggtcgt gagcgcggga aaaccatccc
     8761 caagcggaaa ccgtgcctgt gcgccgaaga ctgaatacgt tcccgtgccg gtgcgatcgg
     8821 ccttgaactt gccttcttcc aaaaccagtt tcagcaattg gtgatattga atcatgcgac
     8881 gaatcaagct ccgcgttttt ccgtcttgcc cggcccatct ttagccggaa tcttaatcat
     8941 gttccatttt ttggcaactt catccagcac caccaccatc aggctttgca ggttgcgctt
     9001 ttcccaaacc aggaaccagg caaaaaccgc cacgctcaaa agcatcagcc gcaaccatgg
     9061 caaccattta cccaccacgc cgatgacaag caactcaaaa cccaacgcgc tccaaaacgc
     9121 gaccttcgcc tgcaacgacc agcgcgcgcg cagccggcaa cggaggattt ttttgccgcc
     9181 cgcacaatcc tccgcgacgg tggtcaattg caaccgcgtc cagcggcttc ccaacacctc
     9241 aatgtcatag tcgctccagc cgatgtccga tttgttcggc cagccttcct tgtccagtcg
     9301 ttcgagcacg cccttgacga actcgatgcg gtcaacgccc gcgcctgccc aaaagcacac
     9361 ttcgttcaac gactggccgc tgccgcgcaa tgcaaccgag tcgagcgttt cctttgtggt
     9421 ttgaggcgca agttgaaggc cgagttgacc ttgataacgc gcccaaccgc gaacaatcgg
     9481 ttgcaaaaaa tataacagcg acaccactgc ccgcgaccac caacggcgtt tggtccgggg
     9541 caattgcgcc tgataacccg ccgccacaca cacacccagc gacagcgcaa ggcttgtgat
     9601 tgccacgggc agcaaccacg gaaaggtcga ggaaagcacc agcaacggca gcgtcatcac
     9661 cacattgtat tccaagctgc tgaaaaacga gagcagcccc atcgcctggc cggtgtacaa
     9721 agtttgaaac caactgctcc cgaatactcc gtgatagaca atcggcgcgc gcacgatgat
     9781 gccgtgtttc gatgccgtgt aaatccgtcc gtgccacatg ctcgaaccga aaatgttgaa
     9841 atattcggga tgtttccgca ccagcagtgc ctcggcttcg ccataaccat gctgctgttt
     9901 cagataggcg cgcacagtcg aacggcgata atgccacaca aaaccgcccg gactgaaacc
     9961 gatcttcagc ccgcgttgct gcaaccgcca gcaaaggtcc acgtcatcgc cggccttgtg
    10021 gaaaatggga tcgaatgcgc ccacgtcttc cagcgcccat ttgtaaaacg ccatgttgca
    10081 gcccggtatg tgctcggcct gccggtcggt cagcatcacg tgcgcgggac cgccggggga
    10141 aaccaacacc gcggcagcga cgctcgaatc gtccggcggc aaaaaattat gtccgcctat
    10201 tcccacaaat tcgctgtcga gcagatcgcc cacgagataa taaagccagt cggtgtcggc
    10261 gcggcagtcg gcgtccgtga acgcgatgat ttcacccgtc gctgcagcga tgccggtatt
    10321 gcgcgcgacc gacagcccga gattttcagc atgacgaaaa tagcggattt gcggatgcgc
    10381 cgccacgatc tgcgaggtgc tatcggtcga gccgtcatcc actaaaatca cttcataatc
    10441 gggataattg agccggtcca atgaatcgag gcaggccttg agcgtgcgtt cggcgttgta
    10501 gctcgccacc acaacggaaa cacggggatg ccgcgccagc ggaaagtagg gcgccaaacg
    10561 aaacatcttc tgcaccgcgc gaaacgaatc cttgcgctgc cggtcgcgcg tggtaaggcc
    10621 catcatccaa tcttcaatct gcaggccgcc gcgccaccag tcgtcggtgt aactgaaaac
    10681 aatcgtcccg gccaaacccg cgcgaaaggt cgtctcgatc tgccacgtca agatttcgca
    10741 tttgcgcggc tcgccttcgc gcaacgaatc aatcccaaat tcgccgagca gcagcggctt
    10801 ggtgtccgcg atcatctgca gtcgcgcaag ataatttttg aatggctgct ggttgtgcag
    10861 ataaacgttg aaacagaaaa aatctagcgt ttgcggccgg agaaattccg tcggcggata
    10921 attggtgaac gtgcaaaggc aatcgggatc aatcgccttg gcttcggcga ccagttcgtc
    10981 aatgaattcc gcgaccgccc tcgcgccgct ccagcgcacg atgtccggag gaatttcgtt
    11041 ggcgatgctg taggcgaaca cggctggatg gcccgcgcaa ttcttcgccg cctgtcgcac
    11101 agcctcgcgc gcggcttcct tctctttctc cgaatcgagg aagcaaaggt gcttgttcca
    11161 tgggatgtcc accaacacct tcaggccgta ttccgccgcg agttcgagga accatttcgg
    11221 cggcacgtaa taaacccgga tgaggtttgc gcccagttca cggatttggg caaagtcgcc
    11281 cgcagtttga tttggcggag caaatgtccc ttcgccattg ccggggtcga acggcccgta
    11341 ggagactcct ttcaggtgga acttcttatc gccaaggcga aagaacttcc cgtccacggt
    11401 cacgcgaact gaggagcttg ccggtgcaac catgtgagtt agacggcttc ggtcaatgct
    11461 ttaattcatt gcaaatcttc tgaaactttg gcttttttgg cctttttacg ctgcttgacc
    11521 tgcggagcgt cgccccaaag cccctccaaa ttgtaacggt cccgcacatc ggcccgcatg
    11581 acatggacga tgacatcaaa ataatcgagc acgacccatg ccgtttgcag cgtgccgtcc
    11641 accgcgcgcg ggcgcaaatc atgttcgtcg cgcagctttt cggtgatttc ctccgtgatg
    11701 gcgcgcagat ggggctcgct tgtgcccgat gcaaccacga aatagtcggt caccgacgac
    11761 agctcccgca cgtccatcac cactatgttt tccgccttcc ggttatcggc gaactcccgg
    11821 cacagcaatg caagtttttt tgaatccatt ctctattctg gaacgatggc ccaatccgga
    11881 ctaaagataa agccgataat tgcggatcgc ttccgcaacc gccggcgcaa cgagattttc
    11941 aatcggcaat ccggccttca cgcgggcgcg aatttgcgat gccgacacgc ccagtggaaa
    12001 acccttcaat gtccttccgc gaaatggcgg cgcaaacgtg ggttcctcct gtcccggtcg
    12061 cggtatcgcc acaaactcca ccagacgagc aagttccgca gcttcgcgcc atttgccgag
    12121 cgacggcaca tggtccgcgc caatcaaata atacaattca gcgccgggaa aacgtttcgc
    12181 gtaatcccgc gccgtgtcaa tcgtgtagga aacgccgccc cgcttgattt cctgttcgtc
    12241 aatttcgtaa ttgctcttgc ccgccaacgc caatcgcagg agacgcagcc gttccgcagg
    12301 cggagcgggt tgcgcgtccg gcttgaacgg cgattgcgcc gccgggatga agaacagccg
    12361 ctccaagccg gcttcttcgc aggccgcctg cgcgaccagc agatgcccaa gatgcaccgg
    12421 gtcaaacgaa ccgccatata agccgatttt tttcaagttc agcaacatct gttgattcca
    12481 tcaaaacgtt tctgcgtgac ttccagataa atttcttccg gcgacttcgc gcacgacgct
    12541 ttttttccac cgagcaaatc cacctgaccc tcggcgcatt tgcattgcat cgccagttgt
    12601 tgcgcgcgct ccagatgcgc ttcgccggac aattcccgcg ccaacgcgca gctcagtgcc
    12661 accacgccga gcacattcac cggcttcgat tcggccatgg cataatcggg aagccacacc
    12721 gcctcgctct cgcgcaggca cgccgcgcgt ttgcgcaaca acaacgtcaa ccattcccaa
    12781 acgctgattg caaaaatcgt cgcaaccata atgaggaaaa aggcaaccac cgccgtatcg
    12841 accatttggt taaaatgatc aatccggttc tttcgcaccg cgctttctgc gatttgaaag
    12901 gcggtcgaat cgcccgaatt tcgcgcagcg tcaacggccg cttgtatcga agccacttgc
    12961 agcgaagcca gttgtttgtc cagtccttgc gcgatctgaa gaaatccaat cttcgaaaaa
    13021 ccaggccgtg tctcttgata aaaaattttc tcccatcccg ccgtcatggt gacggaaagg
    13081 agcc
//
LOCUS       DASZMM010000038         8157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_560616, whole genome shotgun sequence.
ACCESSION   DASZMM010000038 DASZMM010000000
VERSION     DASZMM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560616"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VG754_00940"
     CDS             <1..1047
                     /locus_tag="VG754_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF17798.1"
                     /translation="YLLARCDGDVELTNPLKQVCDASRRAAGLTRQLLMFSRKQVLQP
                     KILDLNAVLRNLSNMLTRLLGEHVAVRTKYAESLPSLEADTGMLEQIVMNLSVNARDA
                     MPKGGQITIGTSVQEIDAAYVHQHPDARPCTAVCLEVTDTGSGMDRKTLDRIFEPFFS
                     TKEVGKGTGLGLATVYGIVKQHQGWIEVKSEVGVGTTFKIYLPASDKKAVSSHDTTVQ
                     QKQIPKGRNETILLVEDETILRLWVKELLESFDYKIVEASNGVEALKAYDEQNGKIDL
                     LLTDMVMPEGMTGGDLARQLRTKQPALKVIYTSGYSGEIMGNDKALPDGPFLSKPYPA
                     PQLAKMVRECLDAR"
     gene            1137..1895
                     /locus_tag="VG754_00945"
     CDS             1137..1895
                     /locus_tag="VG754_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HWF17799.1"
                     /translation="MNKFILVLLSSMLAFATANAAEQDTRCFEMRTYHAAPGKLDDLN
                     ARFRNHTMEIFKKHGMENIGYWVPVDNTNNVLIYILAYPSREAREKSWKEFGADPEWQ
                     SVQKASEANGKLVTKVDSVFMTATDFSPAVAPSKASQSRLFELRRYHASPGKLDDLLA
                     RFRNHTCALFEKHGMGQFGYWVPTDQKQGAGETLIYILAHNDKAASDASWKAFRDDPD
                     WTKVKKDSEVNGPLTVQGGVQSVFMVPTDYSPTK"
     gene            complement(1912..3327)
                     /gene="lpdA"
                     /locus_tag="VG754_00950"
     CDS             complement(1912..3327)
                     /gene="lpdA"
                     /locus_tag="VG754_00950"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWF17800.1"
                     /translation="MKEFDYDVAVIGGGSGGYAAARTAADADLSTVVVEGGREVGGLC
                     ILRGCMPTKALLYAAEVKHLAQHPDAWGIHSQKVTFDFAQVMARKNALIQDFANYRQQ
                     QLTEATFKFIRARARFTDAHTLALSTGETLTAKNFVLSTGSVVAPSPLPFLDEVGCIT
                     SDEAVTLTQLPKSLIILGGGSVAVEFAQIFARLDVKVTLIQRSPYVLHEFDIDATDEI
                     EKVFRREGITLYTNTKLTGATRKNNLKQISFLHGGKTVCVEAEEVLFALGRIANTTGL
                     GLENTGVAMDNQRIIADEEMRTSVPHIYAAGDCTGPYEIVHLAVIQGEVAAHNIAQPK
                     KPRRMDYRLVTSVVFTEPQVAQVGLTEKAALAENIPYRAASYPFADHGKSLIMEAKDG
                     FVKLLADPQSGEILGGCCVGPMGGELIHEIIVAMSKRMTVQELAAVPHYHPTLAEIWT
                     YPAEELALEIQGEADIRGSEK"
     gene            3491..3865
                     /locus_tag="VG754_00955"
     CDS             3491..3865
                     /locus_tag="VG754_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17801.1"
                     /translation="MAERATKWQPLSDIDSPFSSISYTFQDDALLVRMIGAKILTLQF
                     SGVVAFRFEQECPGFDPLPRPLPMLNPSHTFPLLVIDESRWLEQFSLIYKDTRHFALI
                     SYDHLVQLIAKSDVETHWENQL"
     gene            4192..4614
                     /locus_tag="VG754_00960"
     CDS             4192..4614
                     /locus_tag="VG754_00960"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HWF17802.1"
                     /translation="MNEIIFPRSPREAMCGWVYLPRFVDKIRLHLAGKLHSDYQENFT
                     KGFDGAWLKAAGVTADQMIEVVKNSVTDGQVADWISKNVKKTDAEKAAFRDFIFKRGT
                     EGGEITERLNMRKKNAGFGHRDDIQCFIDFIDADEKRI"
     gene            complement(4616..5389)
                     /locus_tag="VG754_00965"
     CDS             complement(4616..5389)
                     /locus_tag="VG754_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17803.1"
                     /translation="MTQFIKLPVRVDKPSVSLDCLANESSDSSTSLAAQDNGSFLEAA
                     AEHLRRGAPPNKKHAQDETRETARQIELLRAWASEKGCVISPERLSEFRLVSEGTSEH
                     KVFFDPARNRAIKQTLPGQFGWFPKLDHSRWTLGIATPLDYLERWILFNIIFADDVRL
                     EGVTLQGGTIIIGQKTEPLSLIISQRWHPADDPNHPAPTETEISMFLHKLGFYPLPNS
                     FYGWQRPEDGIVILDARPDNFIKTRDGIAPIDLPLTRKH"
     gene            5423..>8157
                     /locus_tag="VG754_00970"
     CDS             5423..>8157
                     /locus_tag="VG754_00970"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWF17804.1"
                     /translation="MNSLHNLFNTLQKFDLGNGKQGSFYSLPALEKAGVGAISRLPVS
                     IRLVLEAVLRNCDGKKVSEKNIRELAGWQAKAARIEEIPFIVARIVLQDFTGVPLLVD
                     LAAMRSAVARMGKNPKIIEPLVPVDLVVDHSVQVDFAGSADALQRNLEIEFQRNRERY
                     QFLKWGMQAFDTFKVVPPGIGIVHQVNLEYLAKGVLMSNDGVYYPDTLVGTDSHTTMI
                     NGLGIVGWGVGGIEAEAGMLGQPVYFLTPDVVGVHLTGSLREGVTATDLALTVTQMLR
                     KAKVVGKFVEFYGPGAAALPVVDRATIANMAPEYGATMGFFPIDGECMNYLRATGRSE
                     EHCKLYENYYRAQGLFGIPKKGDIEYSQELELDLASVVPSVAGPKRPQDRIELPKMKQ
                     EFASAFSRPLTENGFGKPADAMHQIFHVECHDGPSVGGGSQEPVSGQQSQARNTNPFT
                     ELEMANNRPTPDSMPAPEMKVCGGEIHHGSVLIAAITSCTNTSNPSVMLAAGLLAKKA
                     VERGLTVNPLVKTSLAPGSRVVSDYLNKTGLQPYLDKLGFNLVGYGCTTCIGNSGPLS
                     APIDEAITKNDLVAAAVLSGNRNFEARVHQNIKANFLMSPPLVVAFALAGRADIDMSS
                     EPIGTGKDGKPVLLKEIWPTLKEIAEQMQLALKPEVFQKLYRDFAGQNPKWNEIPSST
                     GNVYEWDTKSTYIQEPPFFSNFGLQPGEIREIKGARALGIFGDSVTTDHISPAGSIKK
                     TSPAGKYLIENGVDYADFNSYGSRRGNDRVMTRGTFANVRIKNLMVPGVEGGVTKIQG
                     ASEPVSIFDAAMNYAKTKTPLIVLAGQEYGTGSSRDWAAKGTNLLGVKVVVAQSFERI
                     HRSNLVGMGVLPLQFKEGTTAQTLKLDGTETYDVVGIGADLKPQQDLTLRITRANG"
BASE COUNT         1863 a         2190 c         2296 g         1808 t
ORIGIN      
        1 tacctgctcg cgcgttgcga cggggatgtg gaattgacca atccgttgaa acaggtttgc
       61 gatgcctcgc gccgcgccgc cgggttgacg cgccagcttc tcatgttcag ccgcaagcag
      121 gtgctgcaac ccaagatcct cgatttgaat gcggtcctgc gcaatctttc gaacatgctc
      181 acgcggttgt tgggagaaca tgtggccgtg cgcacgaagt acgccgaatc gctcccgtca
      241 ttggaggccg acacgggaat gcttgagcag atcgtcatga acctgtcggt caacgcgcgc
      301 gacgccatgc ccaagggcgg tcaaatcacc atcggcacgt ccgtccagga aattgatgcg
      361 gcttatgtcc atcagcatcc ggatgcgcgc ccatgcactg cggtttgcct tgaagtcacc
      421 gacacgggtt ccggcatgga tcgcaagacg cttgatcgta tttttgagcc gttcttttcc
      481 acgaaagaag tcgggaaggg gaccgggctt ggtctcgcca cggtttacgg catcgtcaag
      541 caacaccagg gctggattga agtgaaaagc gaagtgggcg tcggcaccac gttcaagatt
      601 tatcttcccg ccagtgataa aaaagcggtt tcgagccatg acaccacggt tcagcaaaag
      661 caaattccca aaggacgcaa cgaaaccatt ttgctcgtcg aggatgaaac cattctccgg
      721 ctgtgggtga aggagcttct ggagagtttc gattacaaga ttgtcgaagc gtccaatggc
      781 gtggaagcct tgaaggctta cgacgaacaa aacggcaaga tagacctgct gctgaccgac
      841 atggtcatgc ccgaagggat gacgggcggc gacctggccc gccagctcag gaccaagcaa
      901 ccggccctca aggtgattta caccagcggt tacagcggcg aaatcatggg caacgacaaa
      961 gccctgccgg atggcccatt cctgtcaaaa ccgtatcccg cgccgcaact ggcaaagatg
     1021 gttcgcgagt gtttggacgc gcggtgattt gacattcttc tgatctgatt cgcaggcgtt
     1081 ggttgcgctc ctggaaattt ctcgtcctgg gaaaggtgat ttgatacaac cttcccatga
     1141 acaaattcat ccttgtgctg ctttcttcca tgctcgcgtt cgccactgcc aatgccgcgg
     1201 agcaagacac gcgttgtttt gaaatgcgca cttatcacgc cgcgcccggg aagctcgacg
     1261 atctcaacgc ccgatttcgc aatcacacca tggaaatctt caagaagcat ggcatggaaa
     1321 acatcggtta ttgggtgccg gtggacaaca ccaataatgt tctcatttat atcctggcgt
     1381 atcccagccg cgaagcgcgc gaaaaatcat ggaaagaatt tggcgccgac cccgagtggc
     1441 agtcggtgca gaaggcttcc gaagcgaatg gcaaattggt gacaaaggtg gattcagttt
     1501 ttatgaccgc aaccgatttt tctcctgcgg tcgcaccgtc caaagcctcg caatcgcgcc
     1561 tgttcgaatt gcgaagatac catgcttcac cgggcaagct ggacgatttg ctcgcgcggt
     1621 ttcggaacca tacctgcgcg cttttcgaaa agcatggcat gggacaattc ggttactggg
     1681 tcccgaccga ccagaaacaa ggcgcaggag aaacattgat ttacattctt gcccacaacg
     1741 acaaggcagc ctccgatgca tcgtggaagg cgtttcgcga cgatccggat tggaccaaag
     1801 taaaaaagga ctccgaagtc aacggcccgc tcactgtcca aggcggcgtg caatcagtct
     1861 ttatggtgcc gacggattat tcgccgacga agtaagcgac tgatattggc attacttttc
     1921 cgaccctcga atgtccgcct cgccttggat ttccaacgcg agttcctccg ccggataagt
     1981 ccaaatctcc gccaatgtcg gatggtaatg cggcacagcg gcaagttcct gcacggtcat
     2041 gcgcttggac atcgccacga tgatttcgtg gatcaattca ccgcccatcg ggccgacgca
     2101 gcaaccgccg aggatttcgc cgctctgcgg atccgccagc aatttcacga aaccgtcctt
     2161 tgcttccatg atgagcgatt tgccatggtc ggcaaacgga taactcgccg cgcgataagg
     2221 aatgttctcc gcaagcgctg ctttctcggt caaaccaacc tgggcaactt gcggctcggt
     2281 gaaaaccact gaagtcacga gacgataatc catccggcgc ggttttttcg gttgcgcgat
     2341 gttgtgcgcg gcgacctctc cttgaatcac cgcgagatgc acgatttcgt aagggccggt
     2401 gcaatcgccc gcggcataaa tgtgtggaac ggaagtgcgc atctcttcat cggcgatgat
     2461 gcgctgattg tccattgcaa cgccggtatt ttccaagccc agccccgtgg tgtttgcgat
     2521 gcggccaagc gcgaacaaaa cttcctccgc ctccacgcac acagtttttc cgccgtgcag
     2581 gaaggagatt tgcttgagat tgttcttccg cgtcgcgccc gtcaacttgg tgttggtata
     2641 aagagtgatg ccttcgcgcc ggaaaacttt ttcaatttcg tccgtcgcat caatgtcaaa
     2701 ttcatgcagc acataaggac tgcgctggat cagggtcacc ttcacgtcca gccgcgcgaa
     2761 aatttgcgca aattcgaccg cgaccgaacc gccgccaaga atgatgagcg atttcggcaa
     2821 ttgcgtcagc gtcaccgcct cgtcgctggt gatgcagcca acttcatcaa gaaagggaag
     2881 cggcgatggc gcgaccaccg agccggtgct gaggacgaaa ttttttgccg tgagcgtttc
     2941 gccggtgctc aacgcaagcg tatgcgcatc ggtgaatcgc gcgcgggcac ggatgaactt
     3001 gaacgtcgct tcggtcaatt gttgctggcg gtaattggcg aagtcctgga tcagcgcatt
     3061 tttgcgcgcc atgacttggg caaaatcaaa agtgactttt tgcgaatgaa ttccccacgc
     3121 gtcgggatgt tgcgcgagat gtttgacttc ggcagcgtaa agcagggctt ttgtcggcat
     3181 gcagccgcgc aggatgcaca agcccccgac ttcccgtccg ccttcgacga cgactgtgct
     3241 caaatcggca tccgccgcgg tccgcgccgc cgcgtacccg ccgcttccgc caccgatgac
     3301 agcgacatcg taatcgaatt ctttcatcaa cttgaaagat gggattgaag cgcaccgttt
     3361 gcaatgatta tgaagcgctg ctcgatgctg aggataaaag aggatcttgg aaaatcattg
     3421 gtttgttgcc ctgagtttca cggttgacag taaatgccaa gagtggctca gttttgttca
     3481 ttaggcatct atggcagaac gagcaaccaa atggcagcct ttatccgata ttgattcccc
     3541 atttagctct atctcatata cgtttcaaga tgatgcgcta ttggtgcgga tgattggcgc
     3601 caagatttta acccttcagt tttcgggggt tgtcgcgttt cgttttgagc aggagtgtcc
     3661 tgggtttgac ccgcttccac gcccgcttcc aatgctcaac ccgtcacata cattcccatt
     3721 gctggtcata gacgagtctc gatggttgga gcaattcagt ttgatttata aggacacccg
     3781 tcatttcgca ttgataagct acgaccacct cgtccagttg attgccaagt cggatgtcga
     3841 aactcattgg gagaatcaat tataacgact agaagtcggg cgcatctcac tattttgcac
     3901 gtttctgggc tcttgcgaat tgtgtttatg ggccggccac atcgctgtcc ccctcacccc
     3961 agccctctcc ccaaggagag ggggaccatt tggccgctgc tcgataaatc gaacgctctc
     4021 ggtttgcaac cacgacggga tgcgtgctcc ctctccccaa gggagagggc cggggtgagg
     4081 gggaggaaat gttcaaccac tccgttgtgc aagaaactga gatgcgcccc tagaaatcgt
     4141 cagagctatg gcgcggttga caatcctcga ttctccccgg aatattccgg gatgaacgaa
     4201 atcatttttc cccgcagtcc ccgcgaagcg atgtgcggct gggtttatct gccgcgtttc
     4261 gtggataaaa tccgactgca ccttgccggc aagctgcatt ccgattacca ggaaaatttc
     4321 accaagggct tcgacggcgc gtggctcaag gccgccggcg tgactgcgga ccagatgatt
     4381 gaagttgtca aaaattccgt gaccgacggg caggtagccg attggatttc caagaacgtg
     4441 aaaaagaccg atgccgagaa ggcggcgttt cgggatttca ttttcaagcg cggaaccgaa
     4501 ggcggcgaaa tcactgaacg tcttaatatg cgcaaaaaga acgccggttt cggccatcgc
     4561 gacgacattc aatgcttcat tgatttcatt gacgcggacg agaagcggat ttgaatcagt
     4621 gcttgcgcgt caagggaagg tcaattggcg cgatgccgtc cctggtcttg atgaaattgt
     4681 caggacgcgc gtcaaggatg acaatcccat cttccgggcg ttgccagccg taaaatgagt
     4741 ttggcagcgg ataaaaaccg agcttgtgaa gaaacatgga aatttcggtt tcggtcggtg
     4801 ccggatgatt gggatcatct gcaggatgcc atctttgtga aataattagt gaaagcggtt
     4861 cagttttttg gccgatgata atcgttccac cttgcaacgt cacgccttcg agccgaacgt
     4921 catccgcgaa gataatatta aacagaatcc accgctccaa ataatcgagc ggtgtggcaa
     4981 tcccaagtgt ccagcgagaa tggtcgagtt ttggaaacca tccgaattgg ccgggcaagg
     5041 tttgtttgat tgcgcgattt cgagctgggt caaaaaacac tttgtgttcc gaagtccctt
     5101 cagaaacaag acgaaattca gagagccgtt cgggagagat tacgcagccc ttttcagacg
     5161 cccatgcgcg gaggagctcg atttggcgtg ctgtttcgcg tgtctcatct tgagcatgtt
     5221 ttttgtttgg aggagcgcct cggcgtaagt gctcggcggc tgcttcaagg aagcttccat
     5281 tgtcttgcgc agccaggctg gtgctggaat cactgctttc attcgctaaa caatctaatg
     5341 acacagacgg tttgtcaacc cttaccggaa gcttgataaa ctgtgtcaat ccgggttaag
     5401 gtggattcat caagacggaa atatgaactc actacacaat ctatttaaca cacttcaaaa
     5461 atttgatttg ggcaacggca agcagggctc gttttattcc ctgcccgcgt tggaaaaggc
     5521 gggcgtgggc gcgatttcca gattgcccgt ttccatccgc ctcgtgctgg aagccgtgct
     5581 gcgcaattgc gacggcaaaa aggtttctga gaaaaacatc cgcgaactcg ccggctggca
     5641 ggcaaaagcc gcgcgcatcg aagaaattcc attcatcgtc gcgcgcatcg tcttgcagga
     5701 tttcaccggc gtgccgttgc tggtggatct agccgcgatg cgttccgccg tcgcgcgcat
     5761 gggcaagaac ccgaaaatca tcgagccgct cgtgccggtg gatttggtcg tggaccattc
     5821 ggtgcaggtg gattttgccg gcagcgcgga tgccttgcag cgcaaccttg agatcgagtt
     5881 ccagcgcaat cgcgagcgtt accaattttt gaaatggggc atgcaggcat tcgatacctt
     5941 caaggtcgtg ccgcccggca tcggcattgt gcatcaagtg aatctcgaat atctcgcgaa
     6001 gggcgtgttg atgtcgaacg acggcgttta ttatcccgat acgctcgtgg gaacggattc
     6061 gcacacgacg atgatcaacg gcttgggcat tgtcggctgg ggtgtcggcg gcatcgaagc
     6121 ggaagcggga atgctcgggc aaccggttta tttcctgacg ccggatgtcg tgggcgtgca
     6181 tctgaccggc tcgttgcgcg aaggcgtgac tgccaccgat ctcgcgttga ccgtgacgca
     6241 aatgctgcgc aaagcaaagg ttgtcggcaa gttcgtggaa ttttatgggc ccggcgcggc
     6301 tgcattgccc gtcgtggacc gcgcgaccat cgccaacatg gcgcccgaat atggcgcgac
     6361 gatgggcttc ttcccgattg acggcgagtg catgaattat ctgcgcgcga ccggccgcag
     6421 cgaagagcat tgcaagcttt acgagaatta ttatcgcgcg cagggactat tcggtattcc
     6481 caagaaaggc gacatcgaat attcgcagga actcgaattg gatttggcga gcgttgtccc
     6541 gagcgtcgcg gggccgaagc gcccgcagga caggattgaa ttgccgaaga tgaaacagga
     6601 gttcgcctcg gcattttcaa ggccgctcac ggaaaatgga tttgggaaac cggccgacgc
     6661 gatgcatcaa atttttcacg ttgaatgtca cgatggccca agtgtgggcg gtggcagcca
     6721 ggaaccggtc tcaggccaac agtcccaagc gcgcaacacc aatccgttca ctgaattgga
     6781 aatggccaac aatcgcccga cacccgattc catgcctgcg cctgaaatga aagtgtgcgg
     6841 cggggaaatc caccacggct cggtcttgat cgcagccatc accagttgca ccaacacgag
     6901 caatccttcc gtgatgctcg cggcaggttt gctcgcgaaa aaagccgtcg agcgcggcct
     6961 gactgtgaat ccgctcgtaa agacttccct cgcgcccggc tcgcgcgtcg tcagcgacta
     7021 tttgaacaaa accggactgc aaccttatct ggacaagctc gggttcaatc tcgtcggcta
     7081 cggttgcaca acctgcatcg gcaattccgg cccgttgtcc gcaccgattg acgaagccat
     7141 caccaaaaac gatttggtcg ccgccgctgt tttgtccggc aatcgaaatt ttgaagcgcg
     7201 cgtgcatcaa aacatcaagg cgaacttcct catgtcgccg ccgctcgtgg tggcgttcgc
     7261 gctggctggt cgcgccgata ttgacatgag cagtgaaccg atcgggaccg gcaaggacgg
     7321 caaaccggtt ttgctgaaag agatatggcc cacgctcaag gaaatcgccg agcagatgca
     7381 actggcgttg aagcccgagg tgttccagaa attgtatcgc gacttcgccg ggcaaaaccc
     7441 gaagtggaac gagattccgt cgagcacagg caatgtttac gagtgggaca cgaagagcac
     7501 ttacatccag gaaccgccgt tcttctcgaa cttcggtttg caaccgggtg agatccgcga
     7561 gatcaagggc gcgcgcgcgt tgggcatttt tggcgacagc gtgacgaccg accacatctc
     7621 gccggcaggc agcatcaaga aaacttctcc cgccggaaaa tatctcattg agaacggcgt
     7681 ggattacgcg gacttcaaca gctacggttc acgacgcggc aatgaccgcg tgatgacgcg
     7741 aggcacgttc gcgaacgtgc gcatcaaaaa tttgatggtt ccgggcgtcg aaggcggcgt
     7801 gacgaaaatt cagggcgcga gtgagcccgt cagcattttc gatgcggcga tgaattatgc
     7861 gaaaaccaaa acgccgttga ttgtgctggc cggacaggaa tacggcactg gcagttcgcg
     7921 cgattgggcg gcgaaaggaa caaatcttct gggcgtgaaa gtggtcgtgg cacagagctt
     7981 cgagcgcatt catcgttcca atctggtcgg catgggcgtg ttgccgttgc aattcaagga
     8041 aggcacgacc gcgcagacgc tcaagctgga tggcaccgag acgtatgatg tggttgggat
     8101 tggcgcggac ctgaagccgc agcaagacct cacgttgcgc atcacgcgcg cgaacgg
//
LOCUS       DASZMM010000039         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1934870, whole genome shotgun sequence.
ACCESSION   DASZMM010000039 DASZMM010000000
VERSION     DASZMM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934870"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VG754_00975"
     CDS             complement(<1..236)
                     /locus_tag="VG754_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17805.1"
                     /translation="MKRMNKVFKIFLLLALLGLGYWLWTVLFPNPQQVIRNRLNKLAS
                     LASFSSDDGNISRVAKIQRLGALFADNVQITIDVP"
     gene            259..1872
                     /locus_tag="VG754_00980"
     CDS             259..1872
                     /locus_tag="VG754_00980"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWF17806.1"
                     /translation="MKKDFADDAPPFGRAQIMVVERHGTIITYDVIFLRAKCFEFRLP
                     IGARVFEIRFLQRLAIDIKSAALKTDQFARKTDDAFDEHDVVAGVADGHDVTPLRFAV
                     KIGEPVEELDSSILKSGDHAGTCDANRRDDKTKNHKNNQRRNDDRAQPKQGALGAAPD
                     GNDLDPIPHSHAFCFQGHNDVFLRLSGNRQNASRSGQADELIPVEGSKPALRDFLFTG
                     LFLRLGFARMRMIGQVDSEEKARNFADYLYVQGIDNQIEQDKNGLWSVWIHREEDLER
                     GRALLNNYMANPADPRFQNHTQAAEEMREQKRREQAEHEKKLKKGRQLFRPMATYGFG
                     PVTSILIFISVIAFMDKYFHGGKIFSQLSLTQYDLKNIDDLGIWQALLTRISNFHELL
                     PEIREGQVWRLVTPIFLHFTILHIFFNMLWLRDLGSMIEARQGSLLFVIQVLIIAVIS
                     NLTQFLFTGGGFGGMSGVVYGLFGYVWIRGKLDPTSGLYLHPTTVAMMMIWLFVCMIG
                     VMGSIANAAHASGLLVGVIWGYLSSLRRR"
     gene            2412..>2968
                     /locus_tag="VG754_00985"
     CDS             2412..>2968
                     /locus_tag="VG754_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HWF17807.1"
                     /translation="MPATYSIGLDYGTNSVRALVVNVSDGEEVAAAVWNYAHGEQGVM
                     LSSDPNLARQHPADYAKGAEITLKQALILAKRKVRNFKPEHVIGLGVDTTGSTPLPVD
                     ADGQPLALERRFAKNPAAMAWLWKDHTSIAEAGEITQLARKLRPQYLAKCGGTYSSEW
                     FFSKILRCLRSSPEVFDAAYSWVELA"
BASE COUNT          702 a          716 c          846 g          704 t
ORIGIN      
        1 ggaacatcaa tcgtgatctg gacattgtcg gcgaacaatg cgccaagcct ctggattttg
       61 gcaacgcggg aaatgttgcc gtcatcggaa gagaacgagg cgagactggc gagtttgttg
      121 agacgatttc gaatgacctg ctgtggattt gggaaaagaa cggtccaaag ccaatacccg
      181 agccccagca gcgcgagcag aagaaaaatt ttgaagacct tgttcatgcg cttcatcggc
      241 cgacggtcag gagccgcctt gaaaaaggat tttgccgatg atgcgccgcc gttcggccgc
      301 gcccagataa tggttgttga acggcatgga acgattatca cctacgacgt aatattcctt
      361 cgggccaagt gttttgagtt ccgattgcca atcggcgctc gggtatttga gatacggttc
      421 ctccagaggc ttgccattga cataaaatct gccgccctga aaaccgacca attcgcccgg
      481 aagaccgacg atgcgtttga cgagcatgat gtggtcgccg gagtagcgga tggccacgat
      541 gtcaccccgt tgcggttcgc tgtgaagata ggcgagccgg ttgaggaact tgattcctcg
      601 atcttgaaaa gtggggacca tgctgggacc tgtgatgcga accggcgtga cgacaaaacg
      661 aagaaccaca aaaataatca acgccgaaat gatgaccgcg ctcagccgaa acagggtgcg
      721 cttggggcgg cgcccgatgg caatgacctg gacccaatcc cgcactccca tgctttttgt
      781 ttccaaggac ataatgacgt tttcttgagg ctatccggaa atcgccaaaa tgcaagccgc
      841 agcgggcaag ctgatgaatt aattccggtt gaaggatcga agccggcgct ccgggatttc
      901 ttgtttacgg gtttgttttt gaggttaggc tttgcacgta tgagaatgat cggccaagtg
      961 gacagtgaag agaaggcgcg gaattttgcg gattatttat atgtgcaggg gattgacaac
     1021 caaattgagc aggacaaaaa cggtctttgg tcggtatgga tccatcggga ggaagatctg
     1081 gaacgcgggc gcgcgctgct caacaattac atggcaaacc ctgctgaccc aagatttcaa
     1141 aaccacactc aggccgcgga ggaaatgcgc gagcaaaaac gtcgcgagca ggccgagcac
     1201 gaaaagaaat tgaaaaaagg ccggcaactg ttccggccca tggcgacgta tggttttggg
     1261 ccggtgacat ccattttgat cttcatcagc gtgattgcgt tcatggataa atattttcat
     1321 ggaggaaaaa ttttcagcca gttgtcgctc acgcaatatg atttaaaaaa catagatgac
     1381 ttgggcattt ggcaagcttt gctcactcga atttccaatt ttcacgagtt gctgccggaa
     1441 atccgcgagg gccaggtgtg gcggctggta actccaattt ttcttcactt caccattctt
     1501 cacatttttt tcaacatgct ttggctgcgc gatcttggca gcatgatcga agcgcgccag
     1561 ggaagtttgt tatttgttat tcaagtgttg atcatcgccg tgatttcaaa cttgacccaa
     1621 tttttattca caggcggagg ttttggagga atgtcgggag ttgtttacgg gttgttcggg
     1681 tatgtttgga ttcgcggcaa actcgacccc acttccggac tttacctgca cccgacaaca
     1741 gtcgcgatga tgatgatttg gctgtttgtc tgcatgattg gtgtcatggg ctcgattgcg
     1801 aacgcggctc acgcctccgg gctattggta ggggtcattt ggggctatct gtcgagttta
     1861 agacgccgtt agaccgaatg gctaatgact aaaaatgaga caatcgggtc agattttggg
     1921 gcagtggcac agggtggaaa gaggcagaaa atcggtgttt ttccggcccg cgaagaagcg
     1981 cggcgtcatt tgaatcacgc ggttgcgttc gcctattggc gcgcagaatc gcgattcagt
     2041 aaactgctca gccaatggaa gctgggccgt ttggcgcttt tccaaggcgt taccggcaac
     2101 gtggcctgtt cgcgaatgat gttgccatac ggcaacggtt cggcatgcca aagttcgcgc
     2161 caggtacgca gaggatgtag cggttcaggg ctgatcttca tagtttttac agcgatgcgg
     2221 tttgtttgcc atcactttcc tgccgcatca cccctgacac taacattttt tgtgccaccc
     2281 aggagatggg gagaaacgga acggggtaag agcttatgtt gccagcctcg gattcgggtt
     2341 ttgacccatg ggttggacgt tttacaaact ccttttcacg cagcaacttt gggcctgaac
     2401 caaagaaact aatgcctgcc acatattcaa tcggtttgga ttacggcacc aactcggtgc
     2461 gcgcgttggt cgtcaatgtg tccgacggcg aggaagtggc cgcggcagtc tggaattacg
     2521 cgcacggcga gcagggagtg atgttgtctt cggacccgaa tcttgcccga cagcatcccg
     2581 ccgattacgc aaaaggcgcg gaaatcacgc tcaaacaagc gttgattctg gccaagcgca
     2641 aggtgcgcaa cttcaaacca gaacacgtca tcggccttgg tgtggacaca acgggcagca
     2701 cgcctttgcc cgtggatgcc gacggccagc cgctcgcttt ggagcgtcgg ttcgcgaaaa
     2761 atcccgctgc gatggcgtgg ttgtggaagg accacacgag catcgccgaa gccggtgaaa
     2821 tcacccaact cgcccgcaaa ttgcgtccgc aatatctcgc aaaatgcggt ggcacttaca
     2881 gcagcgaatg gtttttcagc aagattttgc gctgcctgcg ttccagcccg gaagtgtttg
     2941 acgccgcata ttcgtgggtt gaattggc
//
LOCUS       DASZMM010000040         6521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4408275, whole genome shotgun sequence.
ACCESSION   DASZMM010000040 DASZMM010000000
VERSION     DASZMM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4408275"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1104
                     /locus_tag="VG754_00990"
     CDS             304..1104
                     /locus_tag="VG754_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF17808.1"
                     /translation="MTPRVGQHKEPVFPKLNRGQIALTWIGHASFLVQFTDLSVLIDP
                     NFANWLFLLKRIKRSGLRIEHLPPIDLVLLTHAHFDHFHKPSLRKLPHPKIGVMPWGM
                     GDLARDLGFERIIELDWWESFTHRDWKVTLTPCKHWGARVLRDNHRGYGGFILEHQGR
                     KIYHAGDSAYFDGFKQIGHYFSPEIALLPIGAYRPESFRNVHMGPDEAMKVFHDVRAQ
                     WFVPMHYGTFRLSFEDMDEPPRWLRQLASDGGISNKIRMLEEGVPEVF"
     gene            complement(1141..5001)
                     /locus_tag="VG754_00995"
     CDS             complement(1141..5001)
                     /locus_tag="VG754_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF17809.1"
                     /translation="MSKKLRILMLEDSAADAELVEHQLRKSSLDFTIERVQTEETFAR
                     ALQTILPDIVLSDYALPSFNGLEALRLLRKEESDIPFISLSGTINESQVMELIRLGAT
                     DCVSKRNMAKLVPAIQRALREVEHKRQKRQVEESLRESESRFRAIFEVAGTGIAVEDL
                     KGRIVETNRTLQQMLGYTAEELRQLSRQDFTHGRDHSEDQARFEKLLSGESEHYQLEK
                     RFVRKDGRIIWGRLTVSMTRDAAGQPLFPLVVIEDITKHQRAEEAYLQYAAIFEFSND
                     AIVSQTFDGIIFNWNPAAERVYGYSASEANGRSISIILPPEREDELSQILERIKRGER
                     VDNIETTRVRKEGTLIHVSVTTSPIKDLAGKIIGASTISRDITERKQAEAALRESEEN
                     YRKLVELAPDGILIQCEGKYVYLNSAALEIFGAHSPKQVLGKSILDVVHPDYHETIRA
                     NVTKLIAGKDVPLHEEKLIRVDGSVVDVEATAIPFVYRGKPAFQVVVRNITERKRAEE
                     ALRHSEAGLARAQRIARLGSWELNLQTKGLTWSDETYRIFGLVRGECECSTDLFFKHV
                     HPEDRELVRRTTHEAIRQRKLYQIDHRIILPNGEIKTVSEQGEFVLDDKGVPMRCLGT
                     VLDITERKRAESLSAAFSKLGQRLSSAATAVEAARIIADEADKLFGWDACNLQLCSAD
                     LQTFQTIFNMDMIDGVRRPISPSNEAMKPSPRMKKVLEKGAQLFSDESEAKAVKADPF
                     GDASKHSASIMYAPVRNGIRMVGVFSIHSYSPGAYTQKDLENFQALGDHCGGALERIR
                     AEAENQKLAAFAQFNPNPVLELSANGEINYFNDTALQMARQLGKEHPSELMPGETLAI
                     VNHCLATNESRLRYETIISNRTISWSFFPIESIGVVHCYAADITDRQNLEAQLRQSQK
                     MESVGQLAGGIAHDFNNILTVIQGHTSLLGMTGDLSEDAKDSAQQIGLAAERAANLTR
                     QLLTFSRRQIIQPKNLDLNEVVNNMTKMLRRLLGEDITLHVSYTPCLPLVHADPGMME
                     QILLNLSVNSRDAMPKGGRLFINTSALKVDETYSLQVPEAIPGEYVCLTVRDTGVGIV
                     PEVLPHIFEPFFTTKDVGKGTGLGLATVYGIVQQHRGWIQASSKVNKETVFQIFLPGV
                     AGKNAEDIEKSETKVRGGKETILVVEDEPPLRILVRSVLERYGYKVLDANSGVAALTI
                     WEQHKDQIKLLLTDMVMPHGISGRELASKLLADNPDMKVIYSSGYSLAVVGKDMILQE
                     GINFLQKPYNPRKLGQAIRDCLDG"
     gene            complement(5183..6166)
                     /locus_tag="VG754_01000"
     CDS             complement(5183..6166)
                     /locus_tag="VG754_01000"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWF17810.1"
                     /translation="MTHTKERGGLTKVGTGIAGFDMVTGGGLPEGRTTLIVGGPGSGK
                     TMFALQTLLAGVSRGEPGIFVTFQESPRHIIENASSCGWNLPDLEKDKLVILDSRIRP
                     NVFKSIGLYFSGMLAGIRVVANELRAKHIVFDSVDALFSLMGERTSERQEVFRLRDWL
                     LESGLTAIITAQVDGNDAPIFHGSLHSAADCVVNFHQLLTGQGPARSLRLVKYRGSGF
                     VERGIPFVIGPSGIEAVASIANTVEASSKNLSILHQEIADNQAVFQDRIRSLTHQLEM
                     KQAELDFLLKEKEKQQSPTRTRQKNPISKQNKDIRKILKIKAPPTIAHKST"
BASE COUNT         1443 a         1746 c         1757 g         1575 t
ORIGIN      
        1 gcctggactc tctgcacttg gctgccaacc aacgctaaaa ttgaagctgc caaaattatt
       61 tttttcatat tttgcctttc attgttaaga tacaccagca actaaaagta tggacggggt
      121 tccattcaat ttattcaatc cgcctaagag ccataattgc aagcgtttat gatgaagcag
      181 cctgtcaaaa caactcgtcc gccacgcacc cgccaaccct tacccaagtc attcaaagac
      241 ctgacacctt ccaaagcgtt caatcctcgc acgttttttc gtgaaatggt ttggaaggcg
      301 tttttgaccc cgcgcgttgg gcagcacaag gagcccgttt ttcccaaatt gaaccggggg
      361 caaatcgcgc tcacatggat cgggcatgct tcgttcctgg tccaattcac ggatttgagc
      421 gtgctcattg accccaattt tgccaactgg cttttccttt tgaaacggat caagcgttcc
      481 ggcctccgca tcgagcatct cccgcccatc gatttggtgc tgctcacgca cgcgcatttt
      541 gatcacttcc ataagccgag cttgcgcaag ctgccgcacc cgaaaatcgg cgtcatgccg
      601 tggggaatgg gtgacctcgc gcgcgacctc ggcttcgaac gcatcatcga actggattgg
      661 tgggagagct tcacgcatcg cgattggaaa gtcacgctca cgccgtgcaa acattggggc
      721 gcgcgtgtcc tgcgcgacaa tcatcgtgga tacggcggtt tcatcctcga acatcagggg
      781 cgaaaaattt atcacgccgg cgacagcgcc tacttcgacg gcttcaaaca aatcggccac
      841 tatttttcgc cggaaatcgc gttgttgccg attggcgcgt atcgcccgga atcgttccgc
      901 aatgtccata tgggtccgga cgaagcgatg aaagtgtttc acgatgtccg cgcgcaatgg
      961 tttgtgccaa tgcattatgg aacgtttcgc ctttcgttcg aagacatgga cgaaccgccg
     1021 cgctggttgc ggcaacttgc gagtgacggc ggcatcagca acaaaatccg gatgctcgaa
     1081 gaaggtgtgc cggaggtttt ttaggattta cgttggcaga gaccggacgc gcaaatctgt
     1141 tcagccgtcc agacaatcgc gaattgcctg gcccagcttg cgaggattat atggtttttg
     1201 cagaaagttg atgccctctt gcaaaatcat gtccttgccg acaacggcga gactgtaacc
     1261 gctggaataa atcaccttca tgtccgggtt atcggccaga agtttggacg ccagttcgcg
     1321 tccgctgatg ccatggggca tgaccatgtc cgtaagcaga agcttgattt ggtccttgtg
     1381 ctgttcccat attgtcagag cggcaacacc tgaattggcg tcgagaactt tgtacccata
     1441 gcgttcaagg acgctgcgta caaggatgcg caatggcggt tcatcctcga caacaaggat
     1501 ggtttctttc ccgccacgga ccttggtttc ggacttttca atgtcttcgg cgtttttgcc
     1561 ggcaacgcca ggaagaaaaa tctgaaaaac ggtttccttg ttcaccttgc tgctggcttg
     1621 gatccaaccc cgatgctgct ggacgatgcc atacacggtc gccaagccaa ggcctgtccc
     1681 cttgcccacg tctttcgtgg tgaagaacgg ttcaaaaata tgcggcaaaa cttccggaac
     1741 gattccgacc ccggtgtctc tcaccgtgag acaaacgtat tcccccggaa tggcttcggg
     1801 aacctggagc gaatacgtct cgtccacctt gagggctgaa gtgttgatga acaagcggcc
     1861 gcctttgggc atcgcgtcgc gggaattcac ggaaagattc aacaggattt gctccatcat
     1921 tcccgggtcc gcatggacga gaggcaaaca gggtgtgtaa ctgacatgaa gggtgatgtc
     1981 ttcgccgagc agccttcgca gcattttggt catgttgttg accacctcat tcaagtcgag
     2041 gttcttgggc tgaatgattt gacggcggct gaaagtgagc aattgacgcg tgaggttcgc
     2101 ggctcgttct gcagcaaggc caatctgttg cgcagaatcc ttcgcatcct ccgagagatc
     2161 gcccgtcatg ccgagcagcg atgtgtgccc ctggatgacg gtcaagatgt tgttgaaatc
     2221 atgggcgata ccgccggcaa gctggccgac ggattccatt ttctgtgatt gccgcaattg
     2281 tgcctcaaga ttctggcggt cggtgatgtc ggccgcatag caatgcacca cgccgatgga
     2341 ttctatcgga aaaaaagacc aggaaatcgt gcgattgctg atgatggttt cgtagcgcaa
     2401 cctgctctca tttgtggcga gacaatggtt cacgatggca agcgtttcgc ccggcatcag
     2461 ttcagatgga tgctctttgc cgagctgcct ggccatttgg agcgcggtgt cattgaagta
     2521 gttgatttcg ccattagcgg acaattccag cacgggattg gggttgaatt gcgcgaacgc
     2581 ggcgagcttc tggttttccg cttcggcgcg gatgcgttcc agcgcgccgc cgcaatgatc
     2641 gcccagcgct tgaaaatttt cgaggtcctt ttgtgtgtaa gcaccgggcg aatagctgtg
     2701 gattgagaaa acgcctacca ttcttatgcc attgcgcacc ggagcgtaca tgatggaagc
     2761 cgagtgcttc gatgcatccc caaaaggatc ggctttgacc gcctttgcct cgctctcgtc
     2821 cgagaacaac tgcgcgcctt tctcgagcac ttttttcatt cgcgggctgg gcttcattgc
     2881 ctcattgctg ggtgaaatcg gccggcgcac tccatcaatc atgtccatgt taaagatggt
     2941 ctggaacgtc tggaggtcgg ccgaacagag ctggagattg caggcgtccc agccgaatag
     3001 cttgtccgct tcatcggcga tgatgcgggc cgcttccacc gcggttgccg cggaactcag
     3061 ccgctggcca agcttggaaa aggcggcgct gagactttcg gcgcgtttgc gctcggtgat
     3121 gtccaacacc gttccaagac aacgcatggg aacacctttg tcgtccagca cgaattcacc
     3181 ctgctcgctc acagttttga tttcaccatt tggcaggatg atgcggtggt caatctgata
     3241 caacttgcgc tggcggatgg cctcatgcgt cgtgcggcgg acgagttcgc ggtcctcggg
     3301 atgaacatgt ttgaaaaaca ggtcggtgct gcattcgcat tcgcctctga ccaagccgaa
     3361 aatccggtag gtttcatccg accatgtcaa acccttcgtc tggagattca attcccaact
     3421 gccgagacgg gcgatgcgtt gcgcgcgcgc caagcctgcc tcgctgtggc ggagggcttc
     3481 ctcggcccgt ttgcgttcag tgatgttgcg cacaaccacc tggaaagcgg gcttgcctcg
     3541 gtaaacaaaa ggaatggccg tggcttccac gtccacgaca gagccatcca cacggatgag
     3601 tttttcctcg tgcaacggca cgtctttacc agcgatcaat ttcgtgacgt tcgcgcgaat
     3661 ggtctcgtgg taatctggat gaacaacatc caaaatggat ttcccgagca cctgcttagg
     3721 agaatgcgct ccgaaaattt ccaaagcggc gctgttgagg taaacgtatt tcccttcgca
     3781 ttgaatgaga atgccgtccg gcgcaagttc gaccagcttg cgatagtttt cctcgctctc
     3841 acgcagggca gcctcggcct gtttgcgctc ggtgatgtcg cgagaaatcg tggacgcgcc
     3901 gatgattttc ccggcgaggt ccttgatggg tgatgtggtc acagaaacgt gaatcagcgt
     3961 cccctccttc cgcacgcgcg tggtttcgat attgtccacg cgttcccctc gtttgattct
     4021 ctcgaggatt tgcgaaagct cgtcttcacg ctccggcggg agaatgattg aaatggaacg
     4081 gccgttcgct tcagaagccg aataaccata gacccgttcc gctgcgggat tccaattgaa
     4141 gatgatgccg tcgaaggtct ggctgacaat ggcgtcattg gaaaattcaa agattgccgc
     4201 gtactgcagg taagcttcct cggcgcgttg gtgcttggtg atgtcctcga tcaccacgag
     4261 cgggaacaac ggctggcctg ccgcatcgcg cgtcatggaa acggtcaatc gtccccagat
     4321 gatgcgccca tctttccgca cgaagcgctt ttcaagctga taatgctcag attcgcccga
     4381 gagaagtttt tcaaatcgcg cctggtcctc ggagtggtcg cgcccatggg tgaaatcctg
     4441 ccgggaaagc tgccgaagtt cctccgcagt gtagccgagc atttgctgta gcgtgcggtt
     4501 ggtctcgaca atcctgcctt tcaaatcctc cacggcgatg ccagttcccg ccacttcaaa
     4561 aatcgcgcgg aatcgcgact cactttcacg gagggactcc tcgacttgcc gtttctggcg
     4621 tttatgctcc acttcccgca atgcccgctg gattgccggg accagtttgg ccatgttccg
     4681 tttcgagaca caatctgtcg cgccaaggcg tatcagttcc atcacttgtg actcgttgat
     4741 agtcccggaa agcgagatga aagggatgtc cgactcttct ttgcggagca atcggagcgc
     4801 ttccaagcca ttgaaggaag gcagcgcata atcggaaagg acaatatccg gcaaaatggt
     4861 ttgcaatgcc cgggcaaacg tttcctccgt ttggacgcgt tcaatggtga aatccaagga
     4921 ggatttgcgc aattgatgct ccaccagttc tgcatcggca gcgctgtctt ccagcatgag
     4981 aatgcgcagc tttttactca tgaaagctgg aatgagtgca tgatgaggca acgaaggcgt
     5041 gccgaaaaga cggcgctgcc cccatcaagg tcaaattgaa ggccaaaatg gacaacttat
     5101 cgcgagaata agcaaaaaac gggccgggag tcaaaatctc tccgtgccaa atgaaaagaa
     5161 ctgaatttga aagggttgcg acttaggtgg acttgtgggc aatcgttgga ggagccttga
     5221 ttttcaatat cttacgaatg tctttatttt gtttggaaat cggattcttt tgacgggtgc
     5281 gggtggggga ctgttgcttt tccttctcct tcaagaggaa atcgagttca gcctgtttca
     5341 tttccagttg gtgggtgagc gagcggatac ggtcttggaa aacggcctgg ttgtcggcaa
     5401 tctcctggtg gagaatggat aagttctttg aagatgcttc caccgtattc gcaatgctgg
     5461 cgaccgcctc aattcctgat ggtccgatga caaatgggat tccgcgttcc acgaaacctg
     5521 agccgcgata tttcacgagt cgcaggcttc gcgcaggtcc ctgtcccgtc aacagttgat
     5581 ggaagttcac cacgcaatca gcggccgagt gcagtgaacc atgaaagatt ggcgcgtcat
     5641 ttccatccac ttgcgcagtg atgatggctg tgagtccgct ttctaaaagc cagtctcgca
     5701 aacgaaaaac ttcctgacgt tcgctcgtcc gctcgcccat caacgaaaac aatgcatcaa
     5761 ccgaatcgaa cacaatatgt ttggcgcgca attcattggc gacaacccgg atgcccgcca
     5821 gcattccgct gaaataaagc ccgattgatt tgaaaacatt tggccggatg cgcgaatcaa
     5881 gtatgaccag tttgtccttt tccaaatccg ggaggttcca gccgcaggaa gacgcatttt
     5941 caatgatgtg gcgagggctt tcctggaagg tgacgaaaat gcccggctcc ccacgggaca
     6001 cacctgcaag gagggtttgc aacgcgaaca tagttttccc gcttcccggc ccgcccacga
     6061 tcaaagtcgt tcttccttca ggcaagccgc cgccagtaac catatcaaat cctgcgatgc
     6121 ccgttcccac cttggtaaga ccgccacgtt cttttgtgtg cgtcatagag gaaaggatgg
     6181 ttaaggggaa aaagaaaaat gacaagcgca aattcgcgcc cgaagttttg aaaaaaaatt
     6241 cccatgctca gggcaaccga aagttttcac tctggaaacc gcgggccgcc aagccggctg
     6301 attgacacat tttggttctc caagcacgct gcttttcatg gatcaacagc ccgcggagtg
     6361 gggatttcat ggcaatttga gacggcacgg atgacaaaaa atcccctcgt tccatttcgc
     6421 ggccttcacc gcactttttt gagcagtgga gcgctttgct cgcctatctc attctgatat
     6481 ttggaattct ggcggtttat tggccgctaa ggggcttcga a
//
LOCUS       DASZMM010000041         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3313723, whole genome shotgun sequence.
ACCESSION   DASZMM010000041 DASZMM010000000
VERSION     DASZMM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313723"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VG754_01005"
     CDS             complement(<1..410)
                     /locus_tag="VG754_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HWF17811.1"
                     /translation="MKILVANLGSTSLKWRLFDFSNGAERMLHKGGFERVTDYGKTIE
                     ECLGLLRDAGHIASEKDLAAVGFKTIAAKNITGCVRLDERVLEAMAMFNGIAPAHNPP
                     YITGVRLFAKVMPGVPLIGLFETAFHQYAPEAVMR"
     gene            complement(433..711)
                     /locus_tag="VG754_01010"
     CDS             complement(433..711)
                     /locus_tag="VG754_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HWF17812.1"
                     /translation="MAQQAIGMIETKGLCASFEAADAALKAANVSFTGWEKIGSGYVT
                     VFFRGDVASVKAATDAGAAAAAQVGQVVSVQVIPRPHEGLAVLGKWLA"
     gene            complement(737..1033)
                     /locus_tag="VG754_01015"
     CDS             complement(737..1033)
                     /locus_tag="VG754_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HWF17813.1"
                     /translation="MNEAIGMIETKGFTGSVEAADAMAKAANITISKTIPIGGGLITV
                     ICKGDVASVKAAVDAGAKAASKIGELVASHVIARPHEDLLKGFLPDLANSGAKK"
     gene            complement(1065..1742)
                     /locus_tag="VG754_01020"
     CDS             complement(1065..1742)
                     /locus_tag="VG754_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HWF17814.1"
                     /translation="MDVNNKNIHRAVVEHIVRQLVYERLGKPLPRQATGTDPLVVNVS
                     ARHCHLTPEAVEALFGAGHKLQPHKWLYQEGQFAAKETVTLIGPRSRVISNLRILGPC
                     RSLNQVELSYTDAIALGFEIPLRPSGNIAGTPGCMLMGPAGFFEMKEGVIRAARHVHM
                     SPANAEFYGVKAADKMRLKIGGPCAISLDEMLVRVDKAFKLEVHIDTDEGNACDLQPD
                     TPCELLK"
     gene            1830..2801
                     /locus_tag="VG754_01025"
     CDS             1830..2801
                     /locus_tag="VG754_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HWF17815.1"
                     /translation="MTAEERQKRIVEYLQKAEFASLEELANQVLASISTVRRDVIEMA
                     VNGTIRRTHGGARLLNTQSDEFAFSRRDTLQLPEKEAIGRACAELIQPGQSVIVDAGS
                     TVYHVARHLKPEALQIITNSLPVANLFASTQQVEVMVSGGIIYPQLGVLVGPLAVAAF
                     SDLHADVAIMSAGGITLEGISNSHALLIDIQRAMLRAAGKVIFCLDHTKFGRRSVAQL
                     CALSEIDIVVSDCVAPADLIAALHRIGLEIFVAQNTEASTSPTPASEAELPLAIPAPK
                     PKAPVAPKPKPAPPLPRRRAPRPIQAKYTSETEPTSVESSDSRMGWD"
BASE COUNT          612 a          877 c          721 g          629 t
ORIGIN      
        1 cgcatcaccg cttccggcgc gtattgatga aacgcggttt caaacaaacc gatcaacggc
       61 acgccaggca tcactttcgc aaacagccgg acgccggtga tgtagggcgg attgtgcgcg
      121 ggcgcgatgc cattgaacat cgccatcgct tccaaaacgc gctcgtccaa tcgcacgcag
      181 ccggtgatat tttttgccgc aatcgtcttg aatccgaccg ccgccaaatc tttttcgctc
      241 gcgatatgtc ccgcgtcgcg caacaaaccg aggcattcct cgattgtttt gccataatcg
      301 gtcacgcgct cgaagccgcc cttgtgcaac atccgctcgg cgccgttcga aaaatcgaac
      361 agccgccact tgagcgatgt ggaaccaaga ttggcgacga gtattttcac aaggcaaagc
      421 cttgacccac cattaagcca accactttcc caaaacggcc agtccttcgt gcggacgggg
      481 aatcacctgc acgctgacga cctggccgac ttgcgcagcg gctgccgcgc ccgcgtccgt
      541 cgcggccttc acgcttgcca catcgccgcg gaaaaatact gtcacataac cgctgccgat
      601 tttttcccag ccggtgaagc tcacgttcgc ggccttcaac gcggcgtccg ccgcttcgaa
      661 tgacgcgcac aggcccttgg tttcgatcat cccgattgct tgttgtgcca taaaataatt
      721 tggggttgag gttggtttat ttcttcgcgc cggaattcgc caggtcgggc aggaagccct
      781 tcaacaaatc ttcgtgcggc cgcgcgatga cgtgcgacgc gaccagttcg ccgatcttgc
      841 tcgcggcttt tgcgccggcg tccacggcgg ccttgacgct cgccacgtcg cccttgcaaa
      901 tcaccgtgat caatccgccg ccgatgggaa tggtttttga gatggtgatg ttcgcggctt
      961 tggccatcgc gtccgctgcc tcgacgctcc cggtgaagcc cttcgtttca atcatgccga
     1021 ttgcttcgtt catttttatt tgaggttgat tgttgtttgt tgattcattt gagtaattcg
     1081 catggagtgt ccggctgcaa atcgcaggcg ttgccctcgt cagtgtcaat atgcacctcg
     1141 agcttgaaag ctttgtccac gcgcacgagc atttcgtcca gcgaaatggc gcaaggcccg
     1201 ccgatcttca aacgcatctt gtcggccgct ttcacgccgt aaaattccgc gttcgccggg
     1261 ctcatgtgca catgccgcgc cgcgcggatg acgccttcct tcatctcaaa aaatcccgcc
     1321 ggccccatca acatgcaacc gggagttccc gcgatgttcc ccgaaggccg caacggaatc
     1381 tcaaaaccga gcgcaatcgc atccgtgtaa gagagttcca cttgattgag cgaacggcac
     1441 ggcccaagaa tgcgcaaatt ggaaatcacc cggctgcgcg ggccgatgag cgtaaccgtt
     1501 tctttcgcgg cgaattgccc ttcctgataa agccacttgt gcggttgaag tttgtgaccc
     1561 gcaccgaaga gggcctcgac cgcctccggc gtgagatgac aatggcgcgc gctgacgttc
     1621 accaccaacg gatccgtgcc cgtcgcctga cggggcaacg gcttgccgag acgctcatac
     1681 accagttgcc gaacgatgtg ttcgacaacc gcgcgatgaa tgttcttgtt attaacatcc
     1741 atattcaact tggcgcattt ctaccaatat tttgaacaga gtcaacaaaa atcttgcaat
     1801 atctttcata atcttccata atgctcacca tgacggctga agaacgccaa aaaaggattg
     1861 tggaatatct gcaaaaagct gagttcgcct ctctcgaaga gcttgcaaat caggtgcttg
     1921 cttccatttc aaccgtccgc cgcgacgtca tcgaaatggc ggtcaatggc acgattcgcc
     1981 gcacgcacgg gggcgcaaga ttattaaaca cacaatcgga tgaattcgcg ttttcgcgcc
     2041 gcgacacact gcaattgcct gaaaaggagg cgattggaag ggcctgcgcc gaattgatcc
     2101 aacccggcca aagcgtcatc gtggatgccg gttcaaccgt ttatcacgtt gcgcggcatt
     2161 tgaaacccga agctttgcag atcatcacca attccctgcc ggtcgcgaat ctgttcgctt
     2221 cgacacagca agtcgaggtg atggtgagcg gcggaattat ttatccgcag ttgggggtgt
     2281 tggtcggacc gctcgcggtt gcggcgtttt ccgatttgca cgcggacgtc gccatcatga
     2341 gcgccggcgg catcacgctc gaaggcatca gcaattccca cgcgctactg atagacattc
     2401 agcgcgcaat gcttcgcgcc gcaggcaaag tcatcttctg cctcgaccac accaaatttg
     2461 gccgccgctc cgtcgcgcaa ctctgcgccc tatctgagat agacatcgtc gtcagcgatt
     2521 gcgtcgcgcc cgccgacctt atcgctgctt tgcaccgtat tggactggaa atcttcgtcg
     2581 ctcaaaatac cgaagcttca acttcaccaa cgccagccag cgaagcagaa cttcctcttg
     2641 cgattcccgc gccaaagccc aaagcgcccg ttgccccaaa accaaaaccc gcgccacccc
     2701 ttccccgccg ccgcgcgccg cgccccattc aggccaaata tacttctgaa acagaaccca
     2761 cttcagtcga atccagcgac agccgcatgg gttgggatta gatggcaatg attagtgcat
     2821 ttccataatg aaatctgag
//
LOCUS       DASZMM010000042         3887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2211365, whole genome shotgun sequence.
ACCESSION   DASZMM010000042 DASZMM010000000
VERSION     DASZMM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211365"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VG754_01030"
     CDS             complement(<1..1163)
                     /locus_tag="VG754_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF17816.1"
                     /translation="MNDLPPAAQGFSSFLKHLFSGWTWRMAWRDSRSSRKKLLLFSSS
                     IVLGIAALTAIGSLGSNLERAVEEQAKNLLGADLALSSRSTFPPEAEDLIRKLGGEQS
                     RETSLSTMIYFVRGEGTRLVQLRALSGGFPFYGKLETEPPEAAEKFRQGGGALLDESL
                     LMQFHAKIGESIRVGDFTTRIVGELKKVPGESVALAAIAPRVYISMNDLPQTGLVRPG
                     SLVRYKVYFKFSPDTDVSKLVEKIQPQLDKFRLGSDTVEQRKRDLGRAMDNLYHFLNL
                     VGFIALLLGGVGVASAIHVHVKEKLGTVALLRCLGGSVSQTFAIYLAQGMALGLFGAI
                     VGAAIGVAIQSALPSVLADFIPFEFQFHTSWIAVGRAVGIGFVICLLFALLPLL"
     gene            complement(1156..1836)
                     /locus_tag="VG754_01035"
     CDS             complement(1156..1836)
                     /locus_tag="VG754_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF17817.1"
                     /translation="MTLNAILEVQRLSKSYPNGSGELTVVKDISFSLQPGATCAILGP
                     SGSGKTTLLGLCAGLDQPTTGEVKIDGVQLGALDEDGRARIRNEMVGFVFQNFQLIPT
                     LTAVENVMVPLELRGRTNVRDEAVELLGRVGLGERLSHYPSQLSGGEQQRVALARAFI
                     NRPKILFADEPTGNLDAETGRLVIERLFALNADLGTTLVLVTHDLELAKRAARIIRLK
                     GGIVASDE"
     gene            2028..2651
                     /locus_tag="VG754_01040"
     CDS             2028..2651
                     /locus_tag="VG754_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWF17818.1"
                     /translation="MIIPGRTCAADAPATASAAERKTIVVLGDSLAAGYGLDPSEAFP
                     ALLQNKIDEAHWNFTVVNAGVSGDTSAGGLRRMDWLLKRKMDVLIVELGGNDGLRGID
                     LASTRTNLQAIIDRARQKYLQVQIVLAGMQMPPNMGEDYTRAFRAIYPELAKANHAAL
                     IPFLLEGVGGRSDLNQPDHIHPTAEGDKIVAENVWKVLRSLLEKMQD"
     gene            complement(2672..3091)
                     /locus_tag="VG754_01045"
     CDS             complement(2672..3091)
                     /locus_tag="VG754_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17819.1"
                     /translation="MSAQNLHQLPGRITGLLFVLLAAGFVAGCTSAKTVAKSEGQGAR
                     RTFHVSYETAWMASRGAIADNDLRVIDLDRRKGSILAKRDIGMTTFGENVGVWVRRVA
                     PELTSIEVVSRHVGPPVLPGQGLEKPILDNIATMLDE"
     gene            complement(3168..>3887)
                     /locus_tag="VG754_01050"
     CDS             complement(3168..>3887)
                     /locus_tag="VG754_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HWF17820.1"
                     /translation="EVIRVYLPPDANTLLSVTDHCMRSRNYVNVVVIGKQPAPQWLSM
                     HDAIEHCTMGLGIWNWASNDQGGEPDVVMACCGDVPTMEVLAAVSILRKEFPKLKVRV
                     INVVDLMRLQPSTEHPHGLTDKDFDVLFTPDKPVIFAFHGYPWLIHRLTYRRANHRNI
                     HVRGYKEEGTTTTPFDMVVLNDLDRFHLVMDVIERVPSLGASAAYVKQMLVKKLIEHR
                     AYIREHGEDMPEVSNWKWPAR"
BASE COUNT          902 a         1125 c         1039 g          821 t
ORIGIN      
        1 agcagtggca ataaagcgaa caacaaacaa atcacaaagc caatgcccac cgctcttccc
       61 acggcaatcc acgacgtatg aaattgaaac tcgaacggaa tgaaatcggc caacacgctt
      121 ggcaacgcgg attgaatcgc cacaccgatg gccgcgccca caatcgctcc gaacaggccg
      181 agcgccatcc cttgtgccag ataaatggca aatgtttgtg aaaccgaacc acccagacag
      241 cgcagcagcg ccacggtgcc gagcttttcc ttcacatgaa catgaatcgc gctcgccact
      301 cccactccgc caagcaaaag cgcaatgaac cccaccagat tgagaaaatg ataaaggttg
      361 tccatcgcgc ggcccagatc gcgcttgcgt tgctcgacgg tgtccgaacc gagccggaat
      421 ttatcgagct gcggctggat tttctcaacg agcttggaga catcagtatc gggactgaat
      481 ttgaaataaa ctttgtagcg cacgagactt ccgggccgga ccaggccggt ttgcggcaaa
      541 tcgttcatcg agatataaac gcgtggcgcg atggccgcca gtgcgacgct ttcgcccggg
      601 accttcttca actcgcccac aatgcgcgtg gtgaagtcgc ccacgcgaat gctctcgccg
      661 atcttcgcat gaaattgcat cagcaacgat tcatcaagca acgcgccgcc gccctggcga
      721 aatttttccg cagcctcggg aggctcggtt tcgagcttgc cgtaaaatgg aaatccgccg
      781 ctcaacgcgc gcaattggac gagacgcgtc ccttcgccgc gcacgaaata aatcatggtg
      841 gaaagcgaag tctcgcgtga ttgttcgccg cccagtttcc gaatcaaatc ctctgcttcc
      901 gggggaaagg tgctgcgcga actcagcgcc aggtctgcgc caagcaaatt ttttgcctgc
      961 tcttcaaccg ctcgttcgag attgctgccg agcgaaccga tggccgtgag cgcggcgatt
     1021 ccaaggacga tggaagagga gaacaacagc aattttttcc ggctcgagcg gctgtctcgc
     1081 catgccatgc gccacgtcca gcctgaaaac aaatgtttca ggaaagaaga aaatccttgg
     1141 gcggctggcg gcaaatcatt catcacttgc aacaatgcct cccttcaagc gaatgattcg
     1201 ggctgcgcgt ttggccaatt ccaagtcgtg cgtcaccaac acaagcgtgg tgccgaggtc
     1261 ggcgttcagc gcgaacaggc gttcaatgac aagacggccg gtttccgcgt ccaagttgcc
     1321 cgttggttcg tcggcaaaaa ggattttggg ccggttgatg aaggcgcggg cgagcgcaac
     1381 ccgctgctgt tcgcctccgg aaagttgcga gggataatgc gacaaacgtt cgccgagtcc
     1441 aacgcgaccc aagagttcca cagcttcatc acgcacattg gtccgtccgc gcaattcgag
     1501 cggcaccatg acattctcga cggcggtgag cgtgggaatg agttgaaaat tctgaaaaac
     1561 aaaaccgacc atttcattgc gaatgcgagc acggccatct tcgtcgagcg cgccgagttg
     1621 cacgccgtca atcttcactt cacccgtcgt tggctggtca agccccgcgc acaatccaag
     1681 cagagtggtt ttgccgctgc ccgatggtcc caagatggcg caagtggccc caggttggag
     1741 cgaaaaactg atgtccttca cgacggtcaa ttcaccgctg ccgttgggat aactcttgga
     1801 caatcgctgg acttccaata tggcgtttaa agtcaccaac ttgttatcac atgattggcg
     1861 ggatacgtca aattgcagcc gtgttgctct ggcgtgcagg agtcgttcat tgtagattga
     1921 gtgccggttg ccgctaagtc gcagtgaccc ggatgaaaag cgccataatg aaacctttgt
     1981 gttcgcgatt tgcaattgtc gcatgccttt cgtgtttaat cggggcgatg attattccgg
     2041 gacgcacttg cgccgcggac gcgcctgcga ccgcatctgc tgctgaacga aaaacaattg
     2101 ttgtccttgg agacagtctt gccgctggct atggactgga tccaagcgag gcttttccgg
     2161 cgttgctgca aaacaaaatt gacgaggcgc attggaattt caccgtcgtc aatgccggcg
     2221 tcagcggcga cacgtccgcc ggcggattgc gccggatgga ctggctgctc aagcggaaaa
     2281 tggatgtgct gattgtggaa ctgggcggca acgacggttt gcgcggcatc gatctcgctt
     2341 cgacgcgcac caatttgcag gccatcattg atcgtgcgcg gcagaaatat ctgcaggtgc
     2401 aaatcgtcct cgccgggatg cagatgccgc ccaacatggg tgaagattac acgcgggctt
     2461 tccgcgcgat ttatccggaa ctggcgaagg caaatcatgc ggcgctcatt ccatttctcc
     2521 ttgaaggcgt cggcggacgt tctgacctga accaacccga ccatatccat ccaaccgccg
     2581 agggcgacaa aatcgtggcc gagaacgtct ggaaagtttt gcgttcactt ttggagaaaa
     2641 tgcaggattg aaccgcggcc attgcccgag ttcattcgtc cagcatcgtt gcgatattat
     2701 ccaagatagg tttttccaaa ccctggccgg gaagaacggg cggaccaacg tgccggctga
     2761 ccacctcaat cgaagtcaat tccggcgcga cccggcgcac ccagacaccg acgttttcgc
     2821 cgaaggtggt catcccgatg tcgcgctttg caaggatgga accttttcgc cggtccagat
     2881 ctatcacacg caaatcgttg tcagcgattg cgccacgcga cgccatccat gcagtttcgt
     2941 aggaaacatg aaacgtgcgg cgcgcgccct gcccttcaga ttttgcaacc gtcttggccg
     3001 aggtgcagcc ggcgacaaaa ccagccgcca acaaaacgaa aagcaatccg gtgattcgac
     3061 cgggcaattg atggagattt tgcgcgctca taacaactcg tgaatttgct cgcataaagt
     3121 catctttgaa acggcatctt tccatggggt atgtacacca atcctttcta acgagcgggc
     3181 catttccaat tgcttacttc cggcatgtct tcgccgtgct ccctgatata ggcgcgatgc
     3241 tcaatcaatt ttttcaccag catctgcttc acataagccg cgctcgcgcc gaggctgggc
     3301 acgcgctcaa tcacatccat taccagatga aaccggtcga gatcgttcaa caccaccatg
     3361 tcaaacggcg tggtcgtcgt cccttcctct ttgtagccgc ggacatggat gttgcgatgg
     3421 ttcgcgcggc gataagtcaa ccgatggatg agccaagggt aaccgtggaa cgcaaaaatc
     3481 accggcttgt caggcgtgaa cagcacgtca aaatccttgt cggtcaatcc gtgcgggtgc
     3541 tcggtggaag gttgcaggcg catcaaatcc acgacgttga tgacgcgcac tttcaattta
     3601 ggaaattcct tgcgcaagat gctcactgcc gcgagcactt ccatcgtcgg cacatcgccg
     3661 caacaagcca tcaccacatc cggttcgccg ccctggtcgt tgcttgccca attccaaatg
     3721 ccaagaccca tcgtgcaatg ctcaatcgca tcgtgcatgg agagccattg cggcgcgggt
     3781 tgcttcccga tcaccaccac gttcacgtaa ttgcggcttc tcatgcaatg gtcggtcacc
     3841 gacaacaacg tgttggcatc cggcggcaga taaacacgga tgacctc
//
LOCUS       DASZMM010000043         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_12012, whole genome shotgun sequence.
ACCESSION   DASZMM010000043 DASZMM010000000
VERSION     DASZMM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12012"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1197
                     /locus_tag="VG754_01055"
     CDS             85..1197
                     /locus_tag="VG754_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF17821.1"
                     /translation="MAANQSAGDLFAIQAENLVRRFGEMEALSRVSLNIRKGEFFSLL
                     GPSGCGKTTLLRIIAGLDVPDEGSLKIGGRDAATIPAHKRPVNTVFQSYALFPHLSVR
                     YNVAFGLRMKKIPETQISERVDRIMALVQISPFADRRPAQLSGGQKQRVALARALVNE
                     PEVLLLDEPLGALDLKLRKELQVELSQLQRRLGITFILVTHDQEEALVMSDRIAVMNA
                     GKIEQLDEVEKLYERPRTRFVARFLGSCNLIDATVAQRPMNNLAVATTPLGDLHFDAG
                     KAGREICIGEKITLAIRPEKIGLVASGAASSNNQFSALIEDVIYSGAQTQYRLRANEQ
                     SLSVSALNASLGNGGYEIGQSATIHLPAEALIVLDD"
     gene            1199..2137
                     /locus_tag="VG754_01060"
     CDS             1199..2137
                     /locus_tag="VG754_01060"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF17822.1"
                     /translation="METGTSNAQNATQTRSIGAFSPAGQFVRSLITSGPGVIWLTVLL
                     LVPLIAVAVISFMSRGDYGETQLPFTLDNYKRLLGFGLLGFDALYPVILARSLILGIG
                     TALACVVAGLPLAFFIARLPERYKNLALTLVIIPFWTNLLIRTYAWQILLAPEGWLTR
                     LVHAVGIGATGDALYPGTFAVTIGMICDYLPFLVLPLYASVEKLDWSLAEAAMDLGAN
                     GAKVFRHAILPQIMPGLIAGMILVFLPATGQFVIPDLLGGAKTVMLGNAIQQQFGQSR
                     DWPFGSAIAVVALALVMIGLWIYARKSASKGEPQLL"
     gene            2134..2925
                     /locus_tag="VG754_01065"
     CDS             2134..2925
                     /locus_tag="VG754_01065"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF17823.1"
                     /translation="MKRTSIPLNIAASLLYLFLYAPLVVVIVYSFNAARFGSGWQGFT
                     TKWYGTLWENSLALSATKNTLLLAISSAAIATVLGTLLGYGLDRFRFPGKKLFQWFLY
                     VPVFIPDIVMAVAMLLFYSLVRQWLGLFELGLATMILAHVTFQIPFVSIVVRSRLIGL
                     DPSLEEAARDLGANEWQTFLRVTLPLIFPGILAGGMLAFTLSLDDFVVSFFTSGPGST
                     TLPILIYSSVKRGITPDINALSTLIVLASVIGTITVTLLQRGRKN"
     gene            3204..>3880
                     /locus_tag="VG754_01070"
     CDS             3204..>3880
                     /locus_tag="VG754_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWF17824.1"
                     /translation="MTLAFTFPLHAASQKLNLFIWSEYIDPKLIAQFEKEQDCKVNID
                     LYEDAESMLAKMQGGGVSLYDVVVPPDHIVPAMVKLNLLAPLRHENIPNLKNLDDKFA
                     SPPYDRGNRYTVAYQWGTVGVFARQPKGGAPITQSWSLFFDPKQQAGAFVLIDSVRDM
                     VGAALKYKGYSLNSTNPKELKEARDLIIEAKARSVGFEGSVGAKNKVLAKTGRVAIVY
                     SGEGVRG"
BASE COUNT          801 a         1105 c         1049 g          925 t
ORIGIN      
        1 atttttttgt agcatttcgc cgcgcgtttg ttacatctgc gcgcagtgca aataaaggca
       61 gagtctgcgg ccgctcaaaa tcggatggcc gcaaaccagt cggccgggga tttattcgcc
      121 atccaggccg aaaatttagt aaggcgattt ggtgagatgg aagcgctttc gcgcgtttcc
      181 ctgaacatcc gcaaaggaga atttttttcc ctgctcggcc cgtccggctg cgggaagacc
      241 accctgctcc gcatcatcgc cgggttggat gttcccgatg agggctccct taaaatcggc
      301 ggacgcgatg ccgcgacgat tcccgcgcac aaacgcccgg taaatactgt attccaatcc
      361 tacgcgcttt ttccccattt gagtgtccgc tacaacgtcg ctttcggttt gcgcatgaaa
      421 aaaattcctg agacgcaaat ctccgaacgg gttgaccgca tcatggcgct tgtgcagatc
      481 tccccgttcg ccgaccggcg accggcgcag ctttccggcg gacagaaaca gcgcgttgcc
      541 cttgcgcgcg cgctcgtcaa cgaacccgaa gtgctcctgc tcgatgaacc gcttggcgcg
      601 ctcgatctga aattacgcaa ggaattgcag gttgaacttt cgcaactcca acgccgcctt
      661 ggcatcacgt tcattctggt cacacacgat caggaagaag cgttggtgat gagtgaccgc
      721 atcgccgtga tgaacgccgg caagatcgag caactcgacg aagtggagaa attgtacgaa
      781 cgcccccgta cgcgtttcgt ggcgcgcttc ctcggctcat gcaatctcat tgatgccacc
      841 gtggcgcaaa ggccgatgaa caatttggct gttgcgacca cgcctttggg cgatttgcat
      901 tttgatgcgg gcaaagccgg tcgagaaatt tgtatcggcg aaaaaatcac tcttgccatt
      961 cgccctgaaa agattggttt ggtcgcatct ggcgctgctt ccagcaacaa tcaattttcc
     1021 gcgctcatcg aggacgtgat ttacagcggc gcgcagaccc aatatcggct gcgcgccaat
     1081 gaacaatcat tgagtgtctc cgccctgaac gccagcctcg gcaatggagg ttatgaaatc
     1141 ggccaatccg cgacgattca tttgcctgca gaagccttga ttgttttgga cgattaaaat
     1201 ggaaaccgga acaagcaacg cgcaaaacgc gacgcaaacc cgttccatcg gcgcattctc
     1261 gcctgcgggc caattcgtgc gctcgctcat caccagcggc ccaggtgtga tttggctcac
     1321 ggtgttgtta cttgtcccgt tgattgccgt tgcggtcatc agtttcatga gccgcggcga
     1381 ttacggtgaa acgcaacttc ctttcacgtt ggacaattac aaacgcctcc tcggttttgg
     1441 cttgcttgga ttcgacgcgc tctatccagt cattcttgcg cgcagcctga ttttgggaat
     1501 cggaacggct ttggcgtgcg tggttgccgg attgccgctc gcatttttca tcgcgcgtct
     1561 ccccgagcgt tacaaaaatt tggcgctgac actcgtgatc attccgttct ggaccaactt
     1621 gctcatccgc acctacgcct ggcagatttt gctcgcgccg gaaggctggt tgacgcggct
     1681 cgtccatgca gtcggcatcg gcgcgaccgg cgacgcgctt tatcccggga ctttcgccgt
     1741 caccatcggc atgatttgcg attacctgcc gtttctggtg ctgccgttgt atgcatcggt
     1801 ggaaaaattg gactggagcc ttgccgaagc cgcgatggac cttggcgcga atggcgcgaa
     1861 agtttttcgc cacgcaatcc tcccgcaaat catgccggga ttgatcgcgg gcatgattct
     1921 ggttttcctg cccgcgacgg gacaatttgt gattcccgac ctgcttggcg gcgcgaaaac
     1981 cgtcatgctc ggcaacgcca tccagcagca attcggccaa agccgcgact ggccgttcgg
     2041 ctcggcaatc gccgtggtcg cgctcgcgct tgtgatgatc ggcctttgga tttatgcgcg
     2101 caaatccgcc agcaaaggag aacctcaact gctgtgaaac gcacttccat cccattaaat
     2161 atcgcggcgt ctctgctcta tttgttcctt tacgcgccgc tcgtggtcgt catcgtctat
     2221 tcctttaacg cggcgcggtt cggctccggt tggcagggat tcacgacaaa atggtacggc
     2281 acgctttggg aaaattcgct cgcgttgagc gcgacgaaga acactttgct gctcgcgatt
     2341 tcaagcgcgg ccatcgcgac cgtgctcggc acgttgcttg gatacgggct cgaccgtttc
     2401 aggtttcccg gcaaaaaact ttttcaatgg tttctttacg tgccggtctt catccccgac
     2461 atcgtgatgg ctgtggcgat gttgcttttt tattcgctgg tcaggcaatg gctgggattg
     2521 ttcgaactcg gccttgccac gatgatcctg gcgcatgtga ccttccagat tccgtttgtc
     2581 agcatcgtcg tgcggtcgcg tctgattgga ctcgacccgt cgctcgaaga agcagcgcgt
     2641 gatttgggcg caaatgaatg gcagacattt ctgcgcgtga cgttgccgtt gatttttccc
     2701 ggcattctcg ctggcggaat gctcgcgttc acgttgagcc tggacgattt cgtcgtcagc
     2761 tttttcacga gcggtcccgg ttcgaccacg ttgcccatcc tgatttattc ctccgtcaag
     2821 cgcggcatca cgccggacat caacgcgctt tcgaccttga tagtgctggc atctgtgatc
     2881 gggaccatca ctgtcacact gctgcaacgc ggcagaaaaa attaactatg aaagtaaccg
     2941 ccaacaaatg ctcacacaat cgccaatctt gtggcgcagg cttgcagcct gctgtatcgc
     3001 agagttgtac tctgcgaggc gcgcgagatg gcgaaccgct cgggaaaatt ccgttgcccc
     3061 gtttgatttt tccgccctgc cgactaaaag tcggcgatac agcagaatgc aattctgcgc
     3121 tactcgggaa agacatttcg accttgagat tcagtggact ttccagtccc ctgctacgaa
     3181 ttcattgcat cctggtcttg ttaatgacgc tggccttcac atttccgctt cacgccgctt
     3241 cgcaaaagct caatctcttc atctggagcg aatacattga tccgaaactc atcgcgcaat
     3301 tcgagaagga gcaggactgc aaggtcaaca ttgatttgta cgaggacgcc gaaagcatgc
     3361 tcgcaaaaat gcagggcggc ggagtttcgc tttatgatgt cgttgttcct cccgatcaca
     3421 tcgtccccgc gatggtgaaa ttgaacctgc tcgccccgtt gcgacacgaa aacattccca
     3481 acctgaaaaa tctcgacgac aaattcgcca gcccgcctta cgaccgaggc aatcgctaca
     3541 ccgtggctta tcaatggggc accgtcggcg ttttcgcgcg acaaccaaaa ggcggagcgc
     3601 cgataacgca aagttggtcg ctgtttttcg acccgaaaca acaggctggc gcgttcgtgc
     3661 tgattgattc ggtgcgtgac atggtcggcg cggcgttgaa atacaaaggt tacagtctca
     3721 attccacgaa cccgaaagaa ttgaaagaag cgcgcgacct catcatcgag gcgaaagcgc
     3781 gctcggtggg ctttgaaggg agcgtgggcg cgaagaacaa ggtgctcgcg aagaccgggc
     3841 gcgtggcaat cgtttacagc ggcgaaggtg tgcgcggcat
//
LOCUS       DASZMM010000044         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_288794, whole genome shotgun sequence.
ACCESSION   DASZMM010000044 DASZMM010000000
VERSION     DASZMM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288794"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..1863
                     /locus_tag="VG754_01075"
     CDS             415..1863
                     /locus_tag="VG754_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF17825.1"
                     /translation="MQKKNLNLPVSPAGLSRRNFIRRTSLAAGAAALYFPYVGRVLGA
                     NDRINVACIGVGGKGSDDTDCVVANGGTLMALCDVDKGHLANKARQHGEQFPGVKLFN
                     DYRDMLDKIGKDIDAVTVSTPDHHHGVAAIRAMKMGKHAFCQKPLVQTVNEARMVRNL
                     AREKKLATQMGNQGSAEDGLRRAVEVIQSGVIGTPRELHVWSNRPIWPQGLDRPMGED
                     PVPPSLDWDLWLGPAKPRPFKQHVYHPFNWRGWEDFGTGALGDMACHTVNMPFRALKL
                     GYPNVIELELCSRIYSEAYPKTSRIRFEFPEREGLPPLKFWWYDGNPSDPLKPLRPDA
                     GVIHEIVNMQDGLPGSGCLVIGDKGKLFSPDDYGAKFFVCMNGKDTFTPGDQDEACKA
                     VAQTLPRVPQGGSITQRHMSEWFKMMKDGTPSYSNFEIAGYLAEVILLGCVALRVGEG
                     KRMEWDGPNMSSPNLPEAAQFVKRENRAGWDA"
     gene            complement(1909..3537)
                     /locus_tag="VG754_01080"
     CDS             complement(1909..3537)
                     /locus_tag="VG754_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016161 - beta-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 14 glycosylhydrolase"
                     /protein_id="HWF17826.1"
                     /translation="MEAPPSVESQERLRHFGLVANEDYVAWGAVERAPGQWDWQQHDA
                     MEHNLHAAGLKYVVYNWVHFPPAWLKEKRTLMRCLEHSKETSYLSIFDPQTIEWYDHF
                     YRNLHEHFGDRIDDVYACILGPYGEGNYPLEVTSWVNMGHCHEGYWCGDDFAIRAFQN
                     AMMKKYSRISRLNRAWNTHHKTFDEVRPPAQISNDNFKPSPEAFPTPQDKRRWLDFIT
                     WYHQAIIDFAEKSLQTVLKYFPASKVRMKPGGNAGGVNPISWGTYCPGYAKMAGRYKV
                     VLQPADCQGAVFGDKWVATAYQFYGVKECTEPAGDLNEHAFVRRMFSDASCGTSQLFS
                     YQFEQHFTNIQKYVHLFTGKPGETSIAVYCPTTLYRLGASLQPSIAAGYPLRDLCEYD
                     VLDELLIGDGALDTHHHKALLIFQGDIVEQSILDKINSFRRAGGKVIVIGDTPIKNVE
                     GKPWSASSKVTRVSAVAKDKKWLKELSAQLAGLPGVDGRLDGLWTCRRGAQLFLFNST
                     AKPVETEVNGESVMVAPYTIWMNEAASDQKLAHK"
BASE COUNT          937 a         1091 c         1096 g          914 t
ORIGIN      
        1 aggatttgga acgcgagaaa gcgcggattg aagacatcat gctgaagctc tatggcaagg
       61 atttgctcga tgagatcatt gaatgaacat caacttttgt aacctgttga aattccgcaa
      121 agtttcataa agtttcataa agttccgtca aaaacacccc cctccccccc atgcgaaccg
      181 aaacaccgaa cgcccaacat cgaacgtcga actctgaatt ggggaaacac caggcggctc
      241 tggaacattc ggtgttcagc gttcagcgtt cgatgttcga tgtttccgga ctgcttctca
      301 gctacgtccc cgcatataca tgtcatggat tgtcccttga ccttgctctt tcaaagcggt
      361 ttactcgcac ctgatcactg aaaatttgaa ttgctcacag aaagaaaaat caacatgcaa
      421 aagaagaatc tcaatctccc tgtttccccg gctggattgt cgcgccgcaa tttcatccgc
      481 cggacttcgc tggcggcggg cgctgccgcc ctttattttc cttacgtcgg ccgcgtgctt
      541 ggcgccaatg accgcatcaa cgtcgcttgc atcggcgttg gcggcaaagg ctccgatgac
      601 acggattgcg tcgttgccaa cggcgggacg ctcatggcgc tgtgcgacgt ggacaaaggc
      661 catctcgcca acaaagcgcg ccagcacggg gaacaattcc ccggcgtgaa gctgttcaat
      721 gattaccgcg acatgctgga caaaatcggc aaggacattg acgcggtgac cgtttccacg
      781 cccgatcatc atcacggcgt cgctgcaatc cgcgccatga aaatgggcaa gcacgcgttt
      841 tgccagaagc cgctcgttca aacggtcaat gaagcccgca tggtccgcaa tcttgcgcgc
      901 gaaaagaagc ttgccaccca aatgggcaat cagggcagcg ccgaggacgg tttgcgccgc
      961 gcagtggaag tcatccagtc cggcgtcatc ggcacaccgc gcgaattgca cgtctggtcc
     1021 aaccggccga tctggccgca aggccttgac cggcccatgg gtgaagatcc ggttcctccc
     1081 tctctcgatt gggatttgtg gctcggccct gcgaaaccgc gccctttcaa gcagcatgtt
     1141 tatcatccgt tcaactggcg cggctgggaa gattttggca ccggcgcatt gggcgacatg
     1201 gcttgccaca cggtcaacat gccgttccgc gcgctcaagc tcggctatcc caatgtcatc
     1261 gaactggaac tttgctcgcg catttattcg gaagcgtatc ccaagacctc gcgcatccgt
     1321 tttgaatttc ctgaacgcga aggtttgcca ccgctcaaat tttggtggta cgacggcaat
     1381 ccgtcggacc cattgaaacc cctgcgtccc gacgcaggcg tcattcacga gatcgtgaac
     1441 atgcaggacg gtctgcccgg cagcggctgc ctggtcatcg gcgacaaggg caagctcttt
     1501 tcacccgacg attatggggc gaagtttttc gtttgcatga acggcaagga cacattcacg
     1561 cccggcgacc aggacgaagc ctgcaaggcg gtggcgcaaa cccttccgcg cgtcccgcag
     1621 ggaggcagca tcacccaacg gcacatgtcg gaatggttca agatgatgaa ggacggcacg
     1681 ccgagctatt cgaattttga gatcgccggt taccttgcgg aagtgattct gctgggttgc
     1741 gtggcgttgc gcgtgggcga aggcaaacgc atggaatggg acggcccgaa catgtcgtcg
     1801 ccgaatttgc ccgaagcagc gcaattcgtg aaacgcgaaa atcgcgccgg ctgggacgcg
     1861 tgatttgaga ttccataccg cggtccggtt tgctccggat cgcggttttt atttatgcgc
     1921 caatttttga tcagacgcag cctcgttcat ccaaatcgta tagggcgcaa ccatgacact
     1981 ttcgccattc acctccgttt ccacaggctt ggcagttgaa ttgaaaagga acaattgcgc
     2041 gccgcgccgg caggtccaaa gaccgtccag tctgccatcc actccgggca agccggcgag
     2101 ttgcgcggac agttccttca gccatttctt gtctttggca acggcggaaa cgcgggtgac
     2161 ttttgaagag gcagaccatg gtttgccttc gacatttttg atgggagtat caccgatgac
     2221 aatcaccttg ccgcctgcgc ggcggaaaga attgattttg tcgagaatgg attgctcaac
     2281 gatgtcgcct tggaagataa ggagcgcttt atgatgatgc gtatcgagcg cgccatcgcc
     2341 aatcaagagt tcatccagca catcatattc gcacaggtcg cgaagcggat aacctgccgc
     2401 gatgctcggt tgaagactcg cgccgagccg gtaaagcgtc gtcggacaat agaccgcgat
     2461 gcttgtttcg ccgggctttc cagtgaacag atggacgtat ttttggatgt tggtgaagtg
     2521 ttgctcgaac tggtagctga aaagctggct cgtgccacag gaggcatcgg aaaacatccg
     2581 gcgcacaaag gcgtgttcgt tgaggtcgcc ggcaggttca gtgcattcct tcacgccata
     2641 gaattggtaa gccgtggcga cccatttgtc tccgaacact gcgccctgac aatccgccgg
     2701 ctgcagcaca accttgtaac gtccggccat cttggcgtag ccgggacaat aagtgcccca
     2761 cgaaatcgga ttgacgccgc ccgcattgcc gccgggtttc atccgcactt tgctcgctgg
     2821 aaaatatttc aggactgtct gaagcgattt ttccgcaaaa tcaatgatgg cctgatgata
     2881 ccaggtgatg aaatccaacc agcggcgttt gtcctgcggt gttggaaatg cctcgggcga
     2941 aggcttgaaa ttatcgttgg aaatttgcgc gggcggacgc acttcatcga atgttttgtg
     3001 atgcgtgttc caagcacgat taagccgtga aatccgcgaa tattttttca tcatggcatt
     3061 ttgaaatgcg cggatggcaa aatcgtcgcc gcaccaatat ccctcatgac aatgccccat
     3121 gttcacccac gaagtcactt cgagcgggta attgccttcg ccataaggtc cgaggatgca
     3181 ggcatagaca tcatcaattc ggtcgccaaa atgttcgtgc aggttgcgat agaaatgatc
     3241 gtaccattcg atggtttgcg ggtcgaagat ggagagatag gaggtttcct tgctgtgttc
     3301 aaggcagcgc atcaacgtgc gcttttcctt caaccaagcc ggtggaaaat gcacccaatt
     3361 gtaaaccaca tatttcaacc cggcggcatg gagattgtgt tccatcgcat catgttgctg
     3421 ccagtcccat tggccgggcg cgcgttccac agcgccccat gcgacgtaat cctcattcgc
     3481 gaccagaccg aaatgtcgaa gccgttcttg ggattcaacc gaaggcggcg cttccatata
     3541 atttgttcgc ggaaaagccc cgattggaga tgcctgaatg aacgagactg acagattacc
     3601 ggttgaaatg ttccaaggcc gcccgtcgaa cgcggcgttg gttagtccag gcaacgaacc
     3661 tgccccgcaa tggaatgcga taggcagcag gatgaacagc agggtccgcc tatggacgca
     3721 ttgagagaca aattttttga ttcgtggagt tatgatgaag cgaggatact cagccaggct
     3781 catttcgtcc cgcgaaatcg tcaactccgt agtggatttc agagaaccgc ctctacagac
     3841 tgtgtccgaa aaattttgga tatgatacgg ggacagtacc tccttgctaa tgttgaaatc
     3901 tgttggaagt tgaggggcaa acgacgatgg aattcggaaa gggcgttcgt cgaacgatcg
     3961 aatgaaataa cagtttcatt ttttaggatt cgtcgagtta aaaaaccggc cacaactgga
     4021 aatcgtgctg cctatccg
//
LOCUS       DASZMM010000045         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1396448, whole genome shotgun sequence.
ACCESSION   DASZMM010000045 DASZMM010000000
VERSION     DASZMM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396448"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..945)
                     /gene="deoC"
                     /locus_tag="VG754_01085"
     CDS             complement(10..945)
                     /gene="deoC"
                     /locus_tag="VG754_01085"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWF17827.1"
                     /translation="MRAIAPSNAQKTSRQTGFTLPTVDQVMVEERAASFTKRSIKTSA
                     KLAGLKMSVSMMDLTTLEGKDTPGKVAHLCQKALQPLDAKYNVPPCGAVCVYPNMVKY
                     ARRFLGDDSPVHVASVATGFPSGQMPLRTRLEETRHAVGDGADEIDMVINRGAFLTGD
                     HALVFDEITAIKEACGPAHLKVILETGELVTYDNVRIASQLAMEAGADFIKTSTGKVS
                     PAATMPVTLVMLEAIRDYFFATGIRIGMKPAGGIRNSKQALHYLVMLKETLGDDWLTP
                     DLFRFGASTLVNDVLLQIVKTVEGNYQSLDYFSLP"
     gene            1175..1531
                     /locus_tag="VG754_01090"
     CDS             1175..1531
                     /locus_tag="VG754_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17828.1"
                     /translation="MNNVAPSRIKAARAIAIAADFLQIALFPLFAEGFVSLLDDLLDG
                     VVCVLLTWLVGWHLAFLPSFVVKVLPVADLAPTWTIAIFFATRQKSVSTQPPTEVYAE
                     PVLPPQLPEGPSTDKR"
     gene            1544..1921
                     /locus_tag="VG754_01095"
     CDS             1544..1921
                     /locus_tag="VG754_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF17829.1"
                     /translation="MLNQAPVIGFIPSRDFKPARAFYEGVLGLRVLSVDAFAMVFDAN
                     GTPIRVTLVGKFEPHPFTILGWRVPRIEETVSQLKSKGVRFERFDGLTQDELGIWAAP
                     SGAKVAWFKDPDGNMLSVSQHPE"
     gene            complement(1922..2326)
                     /locus_tag="VG754_01100"
     CDS             complement(1922..2326)
                     /locus_tag="VG754_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17830.1"
                     /translation="MDRYTQKVHIKAAEHRKILADVNQLAMDIGRCEKTIGSVMAELN
                     SANTKYSGERNTRQEVELLKILLDCAKRKLAWEKQIASLKKRAPALLESMVRVMNDKD
                     FPPSSEMSAEMLNALKLVQGALERLQAVEVSE"
     gene            complement(2550..>3424)
                     /locus_tag="VG754_01105"
     CDS             complement(2550..>3424)
                     /locus_tag="VG754_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HWF17831.1"
                     /translation="SNDFTVVYHLSQSGFPYADSALQYPATSGVAPVLTNGMIGNGCA
                     FNGTSQFLDAGLVNMGKNFTLSAWVNFATNASSEQTIWANKSGGWNVAGFDFYVNSYQ
                     TNDGVIYFDSADGVGGNVSPRTAAHAVSFGQWHLLTGTMDGVNGVAHVYVDGVDQTIN
                     TGVDTAFQVTNYVRCGSLLTGTPGATGNLYFNGLMDEPRIENGVRSPAWVWASWATVA
                     SSNFSTYACVVPAAVTLHCQPGNGQLVLTWTTGTLQSAPAVSGPYTDISNATSPYPLV
                     PSGIQQFFRVRIPR"
BASE COUNT          701 a          937 c          986 g          800 t
ORIGIN      
        1 cacatttgtt cacggcagcg aaaaataatc caaactctgg taattgcctt ccacggtctt
       61 gacgatttga aggaggacgt cgttgaccag cgtgcttgcg ccgaagcgga acaaatcggg
      121 cgtgagccag tcgtcgccga gggtttcctt gagcatcacc agataatgga gcgcttgctt
      181 cgagttgcgg atgccgccgg cgggtttcat gccgatgcgg atgccagttg caaaaaaata
      241 atcgcgaatg gcttccagca tcacgagtgt caccggcatg gtggcggcgg gcgaaacttt
      301 gccggtactc gtcttgatga aatcggcgcc ggcttccatc gcaagctggc tggcgatgcg
      361 cacattgtcg taagtcacca gttcgccggt ttcgagaatg accttcagat gcgccgggcc
      421 gcaggcttcc ttgatggcgg tgatttcgtc aaacaccagt gcgtgatcgc ccgtcaggaa
      481 cgcgccgcga ttgatgacca tgtcaatttc atccgcgccg tcgccgaccg cgtggcgggt
      541 ttcttccaat cgcgtgcgca acggcatctg gccgctggga aaaccggttg cgacagaagc
      601 aacatggacg ggcgaatcgt cgccaagaaa acgccgcgca tatttgacca tgttcgggta
      661 aacgcagact gcgccgcagg gaggaacatt gtatttcgcg tcgagcggtt gcagcgcctt
      721 ctggcaaagg tgcgccactt tgccgggagt gtctttgcct tcgagcgtgg tgaggtccat
      781 cattgagacg gacattttaa gcccggcgag ttttgcgctt gttttgatgc tgcgcttggt
      841 gaaggacgcc gcgcgttctt cgaccatcac ctggtccacc gtcggcaggg tgaatccggt
      901 ttggcgggat gttttttgcg cgttggacgg ggcgatcgct ctcatacgac aagtgttgtc
      961 accggcgcaa cgggagtcaa ttctgattag gggacctttc cccccgaacc tcccataaat
     1021 ccgatgaagg tagcggacat cgcgttgcga tgtccgcttc tgacggacgg cgcagcgcgc
     1081 cgtccctacc aatttactgg attaacgaat tgccattgac gagtgccgcg ctgcggagca
     1141 ccttgaacga gacgccgcaa aaggcatcgc gaccgtgaat aacgttgctc caagccgaat
     1201 caaagccgcc cgcgccattg ccatcgccgc ggattttttg cagatagccc ttttcccgtt
     1261 gtttgccgaa ggatttgttt cgttgctcga tgacttgttg gacggtgtcg tttgcgtcct
     1321 ccttacctgg ctcgtgggct ggcaccttgc ctttctgccg agttttgtgg tgaaggtttt
     1381 gccggtggcg gacttggccc cgacttggac cattgcgatt tttttcgcga cgaggcaaaa
     1441 atcggtttcc acacagccgc cgaccgaggt ttatgccgag ccggttttgc cgccgcaatt
     1501 gcctgaaggg ccgtccacgg acaaacgata gtattctgct gccatgctaa accaagcgcc
     1561 tgtaatcggt ttcattccga gcagggattt caaaccggcc cgagcctttt atgaaggcgt
     1621 ccttggcctc cgggttttga gcgttgatgc ttttgcgatg gtcttcgacg caaacggcac
     1681 tcccattcgc gtgacgctgg tcggaaaatt tgaaccgcat ccgttcacga tcctgggctg
     1741 gagagtcccg cgtatcgagg aaaccgtgtc tcaacttaag tccaagggag ttcgtttcga
     1801 gcggttcgat gggctgacgc aggatgaact gggcatttgg gccgcgccga gcggcgcgaa
     1861 agtggcgtgg ttcaaagacc ccgacgggaa catgctctcg gtgtcgcaac atcctgagta
     1921 atcactcgct gacttccacc gcctgcaatc gttccaaagc gccttggacc agcttgaggg
     1981 cattcaacat ttccgcgctc atttcgctcg aaggcggaaa atctttgtca ttcatgacgc
     2041 gcaccatgct ttcgagcaac gccggggcgc gctttttcaa gctggcgatc tgcttttccc
     2101 aagcgagttt tcgtttggcg cagtccagaa gaattttcag caattcaact tcctggcgcg
     2161 tattgcgttc gccggaatat tttgtgttgg ccgaattcaa ttccgccatg accgagccga
     2221 tggttttttc gcatcggccg atgtccatgg ccaattgatt cacgtcggcc aggattttcc
     2281 ggtgttccgc cgccttgata tgaacttttt gcgtgtagcg atccacgaat agttactaac
     2341 tcgcgtggag catttgcgca attgaaaagc ggccgccccc gccgtcgctt tcgatttccg
     2401 ttcccggcgg caacacagtg caattaaatt ggagctgcgg cacgttgcaa agctcgccga
     2461 atgtgaatgg gccttacacg attttgatca ccaactcgca atgcttttat cgcgtgcatc
     2521 gctgaaaatg ttacaaggtg aaatcgagtt taccttggaa ttctaacgcg aaaaaattgc
     2581 tggatgcccg atggaacaag gggataagga ctggttgcgt tggaaatatc ggtgtaagga
     2641 cccgagacag cgggcgcgga ttgcaacgtg ccggtcgtcc atgtcagcac gagttgtccg
     2701 ttgccgggct ggcaatgcaa agtcaccgcg gcgggaacca cacaggcgta agttgaaaaa
     2761 ttgctgctgg cgacggtcgc ccagcttgcc cagacccacg ccggcgaacg gacgccgttc
     2821 tcgatgcgag gctcatccat caaaccgttg aaatacaaat tgccggtcgc gccgggcgtg
     2881 ccggtcagga gcgaaccgca acggacataa ttggtcactt gaaacgccgt gtccaccccg
     2941 gtgttgatgg tttggtccac gccgtccaca taaacatggg ccacgccgtt gacgccatcc
     3001 atcgttccgg tcagcagatg ccattggccg aagctgaccg catgtgcggc agttcgcggt
     3061 gaaacatttc cgccgacgcc gtccgcgctg tcgaaataaa tgacgccgtc gttggtttga
     3121 tagctgttca cgtaaaaatc aaaaccggcc acgttccagc cgcccgactt gttggcccat
     3181 atggtttgtt cgctgctggc gttggtcgcg aaattgaccc aggccgagag agtaaaattt
     3241 tttcccatgt tgaccaaacc ggcgtcaaga aattgcgagg tgccgttgaa cgcgcagccg
     3301 ttgccgatca tgccgttggt caaaacgggc gcaacgccgg aggtcgcggg atattgcaac
     3361 gcgctgtcgg cgtaaggaaa tccgctctgg ctcaggtgat agacaactgt gaaatcattc
     3421 gagc
//
LOCUS       DASZMM010000046         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3585044, whole genome shotgun sequence.
ACCESSION   DASZMM010000046 DASZMM010000000
VERSION     DASZMM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3585044"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VG754_01110"
     CDS             <1..206
                     /locus_tag="VG754_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17832.1"
                     /translation="TIAPVGTNVLLSWPAYATNYQLEAASTLVPGTNWATLAANLSTN
                     GSTISTQLPATGSKRFFRLARGP"
     gene            264..1325
                     /locus_tag="VG754_01115"
     CDS             264..1325
                     /locus_tag="VG754_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWF17833.1"
                     /translation="MIRNVIFDWSGTLVDDLPAVWKATNYVLEQAEREQLTLEQFRAE
                     FCLPFKKFYDRHVPHISMPQLETWFHSSFREAQDSVIPLPHAREFLEFCRQRGLRTFL
                     LSTVHETHFKVQAETSGFAEYLERCYTGVLDKSKVIHQILEENQLHPDETIFIGDMQH
                     DIDTARHGGIGSCAVLTGYNSLQQLRASEPDVIVEHLGELLSILEQNDLHLKSASQNG
                     DAHRVPIVTVGALILNDAGDVLMVRTHKWSNLWGIPGGKIKWGEESVAALRREIKEET
                     DLDITDIRFVLAQDSIHSKEFYRDAHFVLLNYTARCVGEPRVKLNDEAREFKWVSVAT
                     ALTMPINKPTRVLLEAFKS"
     gene            1359..1709
                     /gene="folB"
                     /locus_tag="VG754_01120"
     CDS             1359..1709
                     /gene="folB"
                     /locus_tag="VG754_01120"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWF17834.1"
                     /translation="MPNIKIIDLEVFYCVGVTDEERAQPQRLLLNVDMNFDFTVAAVS
                     DRITKTIDYFAVSQQLLHFGDGRSWKLIEKVAMNIAEMILSEHRPQGVTVEVKKFAVP
                     QASYVSVVWTQKRP"
     gene            1706..2737
                     /locus_tag="VG754_01125"
     CDS             1706..2737
                     /locus_tag="VG754_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWF17835.1"
                     /translation="MKAVRLVQPGRRLEAQEIPVPQPGARDVLIRVKAAGICHSDAHY
                     RAGKSRVHPLPLTLGHEVAGVVEKTGAEVTRFKMGDRVCVHYLVSCGECEYCNEGSEQ
                     FCSTGAMIGKYRDGGFAEFICVPARSVFALPDEIPFEQGAIMMCSSATSLHALNKSRL
                     KPGESVAIFGMGGLGFSALQLARAFGAGQIFAVDIKRGKLELARAFGAIPVDASQTDA
                     VAEIKRLTGDRGVDVALELIGLPLTMQQAVRSLGIKGRALLVGITDKNFEIAPYSEVL
                     NKEAEIIGVSDHLAQEIPLLIEWVRQGRLDLSQVITQTVPLDAMAINDVLDRLEKFGD
                     DVRVVIKPG"
     gene            complement(2885..>4081)
                     /locus_tag="VG754_01130"
     CDS             complement(2885..>4081)
                     /locus_tag="VG754_01130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HWF17836.1"
                     /translation="KIINPHDAILRVTSTAICGSDVHLYDGVIPSMEEGDILGHEFMG
                     EVVETGPEVKNLKQGDRVVVPFCIACGQCYYCEHNLTSLCDNTNPDAVVNAKLNGFSG
                     GGLFGYSHLYGGYAGGQAEFVRVPFADVGPLKLPEGLSDEQVLFLSDIFPTGYMAAEN
                     CNIQPGQIVAVWGCGPVGQFAIRSAMLLGAHKVIAIDNVPERLRMAESSGALTINSDE
                     DVLDLLKQHTGGRGPDACIDAVGMEAHGSAYDEIKQAVKLETDRPHVLRQIMFACRKG
                     GTLSLPGVYGGIADKIPFGPAFAKGLSIKMGQTHVHRYMPHLLQLIQTGRIDPGFVIT
                     NQVALADAADAYDMFANRRDGCIKVVLKPEELPVPPAAERIRERLEMPADVLEPAAPR
                     SKRLRR"
BASE COUNT          896 a         1122 c         1152 g          911 t
ORIGIN      
        1 tgacgattgc gcccgttgga acaaatgtgc tgctctcgtg gcctgcttac gcgactaatt
       61 atcagcttga agcggcttct actcttgtgc ccggcacaaa ttgggccaca ttggcggcga
      121 atctttccac caacggcagc acgatttcca cccagttgcc ggccacgggc agcaaacggt
      181 tcttccgtct cgcccggggt ccttgaagtc gcaagcgtat ttcggacttt tgccttcgcg
      241 tacggagcgt taggataaga cgaatgatac gcaatgtcat ttttgactgg tcgggaacgc
      301 tggtggacga tttgcccgcc gtttggaagg cgaccaacta cgttctcgaa caggcggaac
      361 gcgagcaact gacgctggaa caatttcgcg ccgagttttg cctgccgttc aaaaagtttt
      421 acgaccgcca cgtgccgcac atctcgatgc cgcaattgga aacgtggttt cacagttcgt
      481 ttcgtgaggc gcaggactcg gtcatcccgc tgccgcacgc gcgcgaattt ctcgaatttt
      541 gccggcagcg cggattgcgg acttttcttt tgagcactgt ccacgagacc catttcaaag
      601 tgcaggccga aacttccggt ttcgcggaat acctggaacg ttgttacacc ggcgtgctcg
      661 acaagagcaa ggtcatccat caaatcctcg aggagaatca actgcatcct gacgaaacga
      721 ttttcatcgg cgacatgcag cacgacattg acacggcgcg gcatggcgga atcggttcct
      781 gcgccgtgct gaccggctac aacagcctgc aacaactgcg cgccagcgaa ccggacgtca
      841 tcgttgaaca cctcggcgag ttgctttcga ttttggagca gaacgatctc catttgaaat
      901 cggcatcgca aaatggtgat gctcatcgtg ttcccattgt caccgtcggc gcgttgatct
      961 tgaatgacgc cggcgacgtt ctcatggttc gcacgcacaa atggtcgaat ctgtggggca
     1021 ttcccggcgg caaaatcaaa tggggcgagg aatctgttgc ggccttgcgc cgtgaaatca
     1081 aggaggaaac agacctcgac atcaccgaca tccgtttcgt gctcgcgcag gacagcatcc
     1141 attccaagga attttaccgg gacgcgcatt ttgtgctgtt gaattacacc gcccgatgcg
     1201 tcggcgaacc gcgcgtcaag ttgaacgacg aggcgcgcga attcaaatgg gtttctgtgg
     1261 caacggcgtt gacgatgccg atcaataaac ccacgcgggt gttgcttgaa gcattcaaat
     1321 cttgagttcc gggattcttc gtccatctta aaaggcaaat gccgaatatc aaaattattg
     1381 atttggaagt tttttattgc gtgggcgtga ccgacgagga gcgcgcccag ccgcaacgtt
     1441 tgctgctcaa cgtggacatg aatttcgatt ttactgtggc cgccgtcagc gatcgcatca
     1501 ccaagaccat tgattatttt gcggtgtcgc agcagttgct tcatttcggt gacgggcgga
     1561 gttggaaact tatcgagaag gtcgcgatga acatcgcgga gatgatctta tcggaacacc
     1621 ggccgcaagg agtgacagtc gaggtgaaaa aatttgccgt gccgcaagcg tcatacgtgt
     1681 ccgtcgtctg gacgcaaaaa aggccatgaa agcggtccgc cttgtccagc cgggacgccg
     1741 tttggaagcg caggaaattc ccgttcccca acccggcgcg cgcgatgttc tcattcgcgt
     1801 gaaggccgcg ggaatctgcc attcggatgc ccattatcgc gcgggcaaat cgcgcgttca
     1861 tccattgccg ctcacgcttg ggcacgaagt cgcgggtgtt gtcgaaaaaa ccggtgcgga
     1921 agtgacgcgg ttcaagatgg gtgatcgcgt ttgcgtgcat tatctggtga gttgcggcga
     1981 atgcgagtat tgcaatgagg ggagcgaaca attctgttcc accggggcga tgattggaaa
     2041 atatcgcgat ggcgggtttg cggaattcat ctgtgttccc gctcgcagcg tgttcgcgtt
     2101 gccggacgaa atcccgtttg aacaaggcgc catcatgatg tgttcctcgg cgacgtcgct
     2161 gcacgcgctg aacaagagcc ggctcaagcc cggcgaatcg gtcgcaattt tcggcatggg
     2221 cggacttggt ttttcggcgt tgcaactggc gcgcgcgttc ggtgccggac aaatttttgc
     2281 ggtcgatatc aagcgtggca aattggaatt ggcgcgcgcg ttcggagcaa ttcccgttga
     2341 cgcttcgcaa accgatgccg tcgctgaaat caagcggctc accggcgatc gcggcgtgga
     2401 cgttgcgctg gaactgatcg gcctcccgtt gacgatgcaa caggccgtcc gttcgctggg
     2461 catcaagggg cgggcattgc tggtgggcat caccgacaag aattttgaaa tcgcgccata
     2521 cagcgaggtg ttgaacaagg aggctgaaat catcggtgtc tcggaccatc tggcgcaaga
     2581 aattcctctg ctcatcgagt gggtgcgaca agggcggctc gatttgtcgc aagtcatcac
     2641 gcagaccgtc ccactggacg caatggcgat caacgatgtg ctcgaccgtt tggaaaagtt
     2701 tggagatgac gtgagggtgg tcatcaagcc gggttaaaat gacgaaccct taaagcacca
     2761 agcgtccaag ttccaaggac cagagaataa tcaaacatca agctgcaaat aacgcacatg
     2821 gattttaagt ttggaatttg gagcttcaat ggagcttgga tgttggtgct tggggtttgt
     2881 tcatttatcg ccgcaatcgt ttgctgcgtg gcgcggcagg ttccagcacg tcggccggca
     2941 tttcgagtcg ttcgcgaatc cgctcggcag cgggtggaac gggcaattcc tccggtttaa
     3001 gcacgacctt gatgcaaccg tcccggcggt tggcgaacat gtcataagca tccgcggcat
     3061 cggccagcgc gacttgattg gtgatgacga aacccggatc aatgcgtccg gtttggatca
     3121 attggagcaa atgcggcatg taacgatgca catgcgtttg tcccattttt atcgagagcc
     3181 cttttgcgaa tgccgggccg aacggaattt tatccgcgat gccgccataa acacccggca
     3241 acgaaagcgt cccacccttg cggcaggcaa acataatctg gcgcaacaca tgcggccggt
     3301 cagtctcaag cttcaccgcc tgcttgatct cgtcgtaagc cgaaccgtgg gcttccattc
     3361 caacggcatc aatgcaggca tccggtccac gcccgccggt gtgttgcttc aacaaatcca
     3421 ggacgtcctc atccgaattg attgtcaacg cgccgcttga ttccgccatg cgcaggcgtt
     3481 cgggaacatt gtcaatcgcg atgaccttat gcgcgccgag caacatcgcg ctgcggatgg
     3541 cgaattgccc aaccgggccg caaccccaca ctgcgacgat ttgccccggc tggatgttgc
     3601 aattctccgc cgccatatag cccgttggaa aaatgtctga caagaacaac acctgctcgt
     3661 ccgataatcc ttccggcagc ttgagcgggc cgacatcggc gaacggcacg cgaacgaatt
     3721 ccgcctggcc gcctgcgtaa cccccataca gatgcgaata cccgaacagc cctccgccgg
     3781 aaaaaccgtt caacttcgcg ttcaccaccg catccgggtt cgtgttgtcg cacagcgaag
     3841 tcaaattgtg ctcgcaataa taacattgtc cgcacgcgat gcagaagggc accacgacgc
     3901 ggtcgccctg ttttaaattt ttcacttccg gaccggtctc caccacttcg cccatgaatt
     3961 catggccgag aatgtcgcct tcctccatgc tcggaatcac gccatcgtag agatgcacat
     4021 ccgagccgca aatggcggtg gaagtgacgc gcaaaattgc gtcgtgcggg ttgatgattt
     4081 t
//
LOCUS       DASZMM010000047        14449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2212789, whole genome shotgun sequence.
ACCESSION   DASZMM010000047 DASZMM010000000
VERSION     DASZMM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212789"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..899)
                     /locus_tag="VG754_01135"
     CDS             complement(198..899)
                     /locus_tag="VG754_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF17837.1"
                     /translation="MKNKDVSEKSVVLVHGAFADASSWRKIIPLLEKEGITVTAVQLP
                     LKSLADDVATTKRVIENQHGEVILVGHSYGGAVITEAGAGNPAVKGLVYVAAFAPDEG
                     EALTGLMERFAPSPLGTAVVPDSAGFLYIDRAKFAAVFANDLPKEESSLLAATQKPLA
                     APIFGEIVKAAAWKNIPSWYVVSTQDNAINPDLERFMAKRIGAKTQELKASHVSFISN
                     PVEIAKVISAAVAGN"
     gene            complement(990..1964)
                     /locus_tag="VG754_01140"
     CDS             complement(990..1964)
                     /locus_tag="VG754_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWF17838.1"
                     /translation="MKAIHLTAYGNPAENVKLVEVSEPNPPSTGEALVRMEYAPIAYS
                     DVLLAEGVYLLRPKLPSVIGGEGVGIVEAIGPGVTSVKVGDRVTIPFGTFTWSEKVVA
                     PAQDLFVVPASIDVKTASMLNINPTTAVLLLSEFVKLKPKDWIVINAANSQIARCIIA
                     IAKSRGLSVVGIVRRSELIPEIEKLGVDFAGVESPELPKQLQTATGGMPIALGLDAVG
                     GPATAMIASVLSPGSHLVCYAWLSGVPIHVSQGDLIVKKLNIHGFWMYYDEFLPKIQA
                     ALAEATKVVASGKLLLPFTAIYKPPQIKEAVEHTLRGGKILLDFNELG"
     gene            complement(1961..2575)
                     /locus_tag="VG754_01145"
     CDS             complement(1961..2575)
                     /locus_tag="VG754_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWF17839.1"
                     /translation="MEIGTIGAGDFAQAFAKRVLKAGHKVKISNSRGPDSLREIVNRI
                     GPGAMAASREEAAACEMVLLAVPWDNVRETLAGLPKWKNQILIDGVNPFHGKAGNFTP
                     ANVGNLSTSQFVGALAPGARVVKAFNHMLVPNLEADPAINGARRVAFISADDDGAKKR
                     VHALLEHCGYSVVDLGNLRDGGLIQQAGGPIAGRDFFETGEYHK"
     gene            complement(2755..3654)
                     /locus_tag="VG754_01150"
     CDS             complement(2755..3654)
                     /locus_tag="VG754_01150"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWF17840.1"
                     /translation="MEKTKSSGRIRALPRSAGSATRSNVGRTGAHELLWHVLFVARCC
                     GSSSRAPFRTMNAVGDGPASPSLTPAKIILRQTRHPREWASPSLSCYATFKSLNFSSV
                     FLRKKQMAKSNVRDKLVATALELFHTRGYNACGVLDITDAAGVPKGSFYNHFKSKELL
                     ALAVLDRYQSHLALDELQDKSKPPIPRLRSLFEHYTTFHVKWKYTRGCLLGNFGAELS
                     SQSPIIRKALKTIFDRWCDAVANVLQEAKERGDIDKHRDPKELARFLYNAWQGAVVNG
                     KVTQSPVPFETFFAVIFNSILKR"
     gene            3704..4672
                     /locus_tag="VG754_01155"
     CDS             3704..4672
                     /locus_tag="VG754_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HWF17841.1"
                     /translation="MPSKKGNNSVRTQPTSVVTGGAGFLGSHLCDLLLARGHKVIAID
                     NFVTGAVDNISHLGGNTNFKFIQQDVTEFIFLDVPVNYVWHFASPASPIDYLEIPIQT
                     LKVGSLGTHKALGLAKNKRARFLLASTSEIYGDPLVHPQKEDYWGNVNTIGPRGCYDE
                     AKRFAEAMAMAYHREHAVETRIVRIFNTYGPRMRLNDGRVVPAFVSQALKNKPITVFG
                     EGKQTRSFCYVSDLIEGIYRLMMSSMVEPVNIGNPHELTMLEFADEIIRAIGSRSRIV
                     HKPLPQDDPKQRRPDITRARTLLKWEPKVSLEEGLKKTIEYFRGKI"
     gene            4922..6769
                     /gene="pilM"
                     /locus_tag="VG754_01160"
     CDS             4922..6769
                     /gene="pilM"
                     /locus_tag="VG754_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HWF17842.1"
                     /translation="MLNSKSFLTVDFGAGTLKLAEFEVNEAGGLRLRQYGLKSLGAEG
                     AKEETRESVLQKALQELLAEKGVRSRDVNVCAPGFHVFSKFVKLPPVDTSKVTQIIQY
                     EAQQNVPFPLSEVVWDYQILGSTATGELEVLLVAIKSDIVEGLFRVSEAAGLRLQLAD
                     VSPAALCNAFRYNYGDLEDCTMLLDIGAKTSNLLFFEKGKVYSRSINLGANSITQDFS
                     NESKLAWGEAEKKKISEGFVSLGGAYEEPENPHQAAISKIARQFMTRLHIQVNQTMQF
                     YRGQQGGSAPVRLFLCGGASLMPYTAQFFAEKLNVPVEYFNPFRNVQIDPSINLEELA
                     RVAHSLGEIVGLGLRNLAHCPVELNLMPESTLNWVQFNQKKPYFIATVFSLVIVVFLS
                     SVVFSKLAGINQEELDKLNEKIQPLKTQEVAFNNALQDRKKAKDVADQYAGWIQDRYY
                     WPDVLTELHQLMIQVEAGTAAKLHTDTGVWIEKFITTSTSDEAANANTPGVPPPTYDS
                     NLPGGRRAPLRRLGEGPPMATPGMPAASSAKSTNEVSVITVLFRGVNLSGVSPTANSD
                     IAYATEAALQASPFFLKTGTHLSGTIDQPGDSGTFTFGMEIQLKTPIKL"
     gene            6771..7775
                     /locus_tag="VG754_01165"
     CDS             6771..7775
                     /locus_tag="VG754_01165"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HWF17843.1"
                     /translation="MTWIKRNLLFVIGGVIAVALMGAGGYYLWTQISLEGSLAGQIAD
                     QYHELDRLIGLNPHPGYKDIDNTKAARAETASIKAYIGKTTPYFQRIAPIPDMGGSNK
                     ISNSDFAAALRVTVNELNHSAEQQSVEVPKDYYFTFEAQRKIMIFDPASLDKLAVQLG
                     EVKAISEILFSARVNSLDYIRREIVSTDDKNQSDYLLTKTVSTPMADLTPYEVRFRCF
                     SAELAIVMANLASSPYGFIVKAINVEPTSGAEEMPGGGLMPNGNPSPQNPNPYRYMQN
                     DGRSRAPVRSLGGPPTAMPVTQSVAAPTSGKPQAFLTEKPFNVTLLIQVVKTKPLPAK
                     "
     gene            7787..8587
                     /locus_tag="VG754_01170"
     CDS             7787..8587
                     /locus_tag="VG754_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17844.1"
                     /translation="MEFLKKNYEKVLLAVVLLGLTIAACLLPFVISSKRAAMENMVSQ
                     RIPHPKPLPPLDMTLEDAALQREQTAFALDFTTKHNLFNPVIWKRIAGGGLLKESTGN
                     EEGAAALEVTNIKALYLELKFGSPSASGYMIDITRQGAPREDLRHKSSYISKESKTDL
                     LTLLEVKGDTNKPSELVLEWNESGEMKGDTISIGPDKPFRKIEAYSADLKYPPEKREW
                     TDRRVGFRFSIANAYYKIVAITPTNVVVLAESNNKKTTITFHPATEPR"
     gene            8615..11338
                     /locus_tag="VG754_01175"
     CDS             8615..11338
                     /locus_tag="VG754_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17845.1"
                     /translation="MIKASKLFCLIFLAAAATSLQAQPVSDPVQAGIDEAIHRQADTI
                     ALRRTLVEAMEARQRGDLLGAHKLYERCYDYMQRIGPGGVEPEAQATIAGLSEVLMTM
                     ARADQKRQDYKEADVKLTRVLVVDPKNQVARDAKRDNQRMLQNQAGTIPSQETIERIP
                     EFRTNQVQIGTLIQDGKLLFEAGHLDEAEAKLKLAYAQDPSSVAAYEYLAAIQQRRIA
                     GASRDTELEANSSLLHVEKAWTKDRSNGDYVPRPNPFNRTNLVYTGKGRQLILSKLDH
                     IHIDSVKYDDLPLTEVINNLSELARQRDPDKLGINFYIDRQTPAAIGAASGAIDPATG
                     LPVAAPLPTDVGDITSVTVKITPSLTDVRLADVLDAITKTASKPIRYSILDYAVSFSL
                     KGQEPVPLETRWFRVDPNTFRMGLESVGGIEFGNITTSSGSGGGGGGGGGGGGGGGGS
                     GSTTTAILPQVNVTGSTTTGGGGGGGGGGQSGGGQSGGGLRYVTSRTNETALLQVMVR
                     QFFNSVGLDFNSNNPANIGKTFVFNDRKGILVVRSTAQDLDMIDAAIQALNQAPPEVN
                     IKAKFIEITQNDNRGLGFQWTLGNTQIGSAAVASGGTQPSLNGTSTTVNPEGTFPGSS
                     IFGTTTPPSGTDGVLTSGLRNTFGSAGTTLPTVGTLTGILTDPQFRVAINALEQRDGV
                     DELNAPEVTTESGRQAQIQAVDVQTIVTGNQVTASTGSSVSGVANGINSPIVQAVPTI
                     NYSTSPIPFGPVLDVVPYVSADEFSVQMTLIPTITEFVGYDSPGNFVPEALVSGPNAQ
                     AASIQGALPLPHFRMRQVTTSVTVWDSQTVVLGGLLTDSVTKIKDKVPMLGDLPLVGR
                     LFQSQSSSKTKKNLMIFVTPTIINPDGTRFHSDEEMPFAQGGVPVQQPVIR"
     gene            11576..13507
                     /locus_tag="VG754_01180"
     CDS             11576..13507
                     /locus_tag="VG754_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17846.1"
                     /translation="MSQLQRAFKTLGGLVLLVAFPFAIFGQTNNSTNFYAAQAGEYVP
                     AGNLPGDQTHSSLALNASGGYLVWQDNITDGDGLGISAVRLDSTFSPTLGNFRVNQQG
                     AADQENPQVALLNGGGAVFVWQSGKQSFQHIMARFLGSNGLWTTGDVQVNTATNYQVN
                     PAVAVLTNGNVIVTWGSYGEDNADGLQGVYGQMLSPTGQKVGTEFRINQFTPYNQRTP
                     AVAALPNGNFLVAWVSELERTVQTSFDHIPSGMAAFSVDIYARQFNSAGTAQGGEFLV
                     NVLTNICANPAIGVGSDGTYFIAWSQKDSVTVNNSWDVWGRQFSKIGVGGAPALINTQ
                     VYGDQYAPKVSSIGTDYLVVWTSMGQDGSREGVYGQFLHGNGSHVGGELRVNTTVLNQ
                     QMFPAVASDGVGRFMVSWSSYVGGIDSLDLNAQRYATTQTPLTAPGAPIVLALDSYSL
                     SVTWPPVAGFSVSYYELFVDGSSTPINVTNNMWSYLNYNPSSTHTFQLAYVLTNGNTS
                     PLSATATGTTWGVDRNFDGLPDDWEAKYYGTNQANWPKGGASTVLAPGVTLLDVFLSG
                     ANPSDPSTWLKETITKTSEGYFLNWNTIPGGIYQVRGSTDLQTWTNLGSQRFEAGNTD
                     SIYLGLSTKGYYQVLRNRY"
     gene            13515..>14449
                     /locus_tag="VG754_01185"
     CDS             13515..>14449
                     /locus_tag="VG754_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17847.1"
                     /translation="MFHFIKRFWVVLALTLAGAQTMFGFAMLGPFNEGFQVPAIGYNL
                     TLPAASFGNDGNNADVGAPKNYGQGYRWNTPTNYYAFDESFSRYFGATGESNIDAAFA
                     MFNNLTNVSNLNLDQWPLNASRINYRAQALQLLDLKSYTMSIIIEQLGLAQPDRWVWC
                     LHDRFTGGATCPGFEYSVIQRNYDPYTYLYSSYVNGVFYDYQIEELCALTPLNPFAPL
                     LADAIEIPVDPTTYQDTTDAVAAYGSEYIYGSFFLSFTRDDIGGLKCLYSATNAYPEM
                     AESNSVQIVTNTAQPVLLVTSNLAIFESQALTNDDA"
BASE COUNT         3322 a         3835 c         4052 g         3240 t
ORIGIN      
        1 cttttactga atgcgaaatg aggctacgtg aacaattgtt tgcatggatc ccgacaacct
       61 ttgctcgttc ctcccatgct tagcggtagc tagggagcgt ctcgcaacac cacgccgcaa
      121 cgggcttcag aggggcaagt aaaaacgcca aagcgacgcg agcgttcgtc gcggcgtaac
      181 gctcttgttg agtgaggtta gtttcccgcc acggcggctg aaatgacttt cgcaatttca
      241 actggattgg atataaaact cacgtggctt gcttttaatt cctgtgtttt agcccctatt
      301 cttttggcca tgaatcgctc caaatcggga ttaatggcat tgtcctgtgt tgatacgaca
      361 taccatgaag gaatgttctt ccatgcggca gccttgacga tctctccaaa aattggggca
      421 gcgagaggtt tctgtgtggc cgcgagcagc gacgattcct ctttgggcaa gtcgtttgca
      481 aatactgctg caaacttagc gcggtcgatg tagagaaatc cggccgaatc cggcacgacc
      541 gctgttccta acggtgatgg cgcaaatcgt tccatcaaac ctgtcagggc ctcgccttca
      601 tcaggcgcga atgcggccac atagaccagc cccttcacag cggggttccc agctccggct
      661 tctgtaatga ccgcgcctcc gtacgagtgc cccaccaaaa tcacctcgcc gtgttggttt
      721 tcaattactc gtttagtggt cgcgacatcg tcagccaggg atttgagcgg gagttggacc
      781 gcagtcacgg ttatcccctc cttctcaagg agaggaataa tttttcgcca actcgacgcg
      841 tctgcaaacg cgccatgaac cagcacaacg gatttttctg agacgtcttt atttttcatt
      901 gtcgttcctt cgtttagtat ttttaaaccg tttcacttct gaatgtcttt cgtagatgtt
      961 gtgttcacaa aatctttgat cgtccgacgt cagcctagct cattgaaatc caacagaatc
     1021 ttgccaccac gcagggtgtg ttcgactgct tctttgattt gaggcggttt gtagattgcc
     1081 gtgaatggca acagtaattt gcccgaagcg actacttttg ttgcttcagc cagcgccgct
     1141 tgaattttgg gaagaaactc atcgtaatac atccagaagc cgtgtatgtt caattttttg
     1201 acgatgaggt cgccttgcga cacgtgaatt ggcacaccac tcagccacgc atagcagacg
     1261 agatgggagc ccggcgaaag cacacttgcg atcattgctg tcgcgggacc gcccacggca
     1321 tcaagaccca gagctattgg cattccgccg gttgcagtct gaagttgctt tgggagttcc
     1381 ggcgattcga caccggcgaa atcaaccccc aatttttcaa tttccgggat aagctcggac
     1441 cgtcgcacaa tacccacaac gctaaggccg cgtgattttg caatcgcgat tatacaccgt
     1501 gcgatttgag agttggctgc attgattaca atccagtctt taggctttag ctttacaaat
     1561 tcgctcaaga gaagaacagc tgtcgtcgga ttgatattta acatcgaagc cgttttgaca
     1621 tcaatcgaag caggaacgac gaaaagatct tgggctggag caacgacttt ttcagaccaa
     1681 gtgaatgttc caaatggaat tgtcacgcga tctccaacct tgacgctggt gactcccggc
     1741 ccaatcgcct cgacgatccc cacgccttcg cccccaatga cagaaggcag cttcgggcgt
     1801 aaaaggtaga ctccttccgc taaaaggacg tcgctatagg caatgggcgc gtattccatc
     1861 cgcaccagag cctcgcccgt agatggcggg tttggctccg aaacttcgac caactttacg
     1921 ttctccgctg gattgccgta ggctgtgaga tggatggctt tcatttatga tattctcctg
     1981 tctcgaaaaa atcccgaccg gcgataggac cgcctgcttg ctgaattaat cctccatcgc
     2041 gaaggtttcc aagatcaacg acagaatagc cacagtgttc aagcaacgca tgaacacgct
     2101 tcttcgcgcc gtcatcgtct gcggaaataa atgcgactcg tctcgctccg ttaatagcag
     2161 ggtccgcctc caggttggga acgagcatgt ggttaaacgc cttgaccact cgtgctccgg
     2221 gggccagcgc cccaacaaac tggctggtgg acaggtttcc aacatttgct ggcgtgaaat
     2281 ttccggcctt gccgtgaaaa ggattaaccc cgtcaatcag aatctggttt ttccatttgg
     2341 gaagacctgc aagtgtctcg cgcacgttat cccaaggcac ggcaagaagc accatttcac
     2401 aagccgccgc ctcctccctg gacgcagcca ttgctccagg accaattcgg tttacaattt
     2461 cccggaggct gtcggggcct cggctattgc tgattttcac cttatggcct gccttcaaca
     2521 ctcgctttgc gaatgcttgc gcgaaatctc ctgcaccgat ggttccaatt tccatacact
     2581 atcctttttc atttggactt ttcagtaaaa ccatgcggct ttgcatcaga atcctcttca
     2641 cttcagcgcc gcgcacaaac tggaactggt tttgcaacct ttaagtatta aatatgaccg
     2701 gtcgtctcaa accgtcaagg gttaaaattc tcaatcgcgt cagcaaaagg aatcttatcg
     2761 tttcagaatg gaattgaaga tgaccgcaaa gaaagtttca aagggcaccg gactttgagt
     2821 gactttgcca ttaacgacag cgccctgcca tgcgttatac agaaaacgag ccaactcttt
     2881 cgggtcgcgg tgcttgtcaa tatctccccg ttctttcgcc tcctgcaaga cgttcgcgac
     2941 cgcatcgcac cagcgatcaa aaattgtttt gagggctttt cggatgatgg gactttgtga
     3001 agagagttcc gctccgaaat ttcccagcaa acatcctcgc gtgtatttcc attttacgtg
     3061 aaatgtcgtg taatgttcga aaagtgagcg caagcgtgga atagggggtt tgcttttgtc
     3121 ctgaagttcg tccagtgcga ggtgactctg gtaacggtcc aaaacagcca gcgccagaag
     3181 ctctttgctt ttaaagtgat tataaaacga gcctttcgga actcccgcgg catcggtgat
     3241 gtccaagaca ccacaagcgt tgtagcctct ggtatggaag agctccaatg cggttgctac
     3301 taatttgtca cgaacatttg atttggccat ttgcttcttt cgaaggaata cagaagagaa
     3361 atttaggctt ttgaaggtgg cgtagcaact taaactgggg ctggcccact ctcgtggatg
     3421 gcgtgtttgc cgcaggatta ttttggcggg cgttaaggag ggactggccg ggccatcgcc
     3481 gaccgcgttc atcgtgcgaa acggagcgcg gctcgatgag ccgcagcaac gcgcgacgaa
     3541 caggacgtgc cagagtaatt catgcgcacc tgtccttccc acattgctgc gggtcgccga
     3601 ccccgcgctc cggggcaggg cgcggatgcg cccggacgat tttgtttttt ccacttttgg
     3661 ctttcatcgt tgcggcttgg ctgtaggatg cacgcctatt tttatgccgt cgaagaaagg
     3721 taacaattct gtacggacgc agcctacttc tgtagtcacg gggggcgcgg gttttttggg
     3781 gtcgcatctg tgcgacctgc tgctggcgcg cgggcacaag gtgatcgcga tcgataattt
     3841 cgtgacggga gcggtggaca acatctcgca cctgggcggc aacacgaatt tcaaattcat
     3901 ccagcaggac gtgacggaat ttattttcct ggatgtgccg gtgaattatg tgtggcactt
     3961 cgcgtcgccg gcgagcccga ttgattattt ggagataccg atccagacgt tgaaggtggg
     4021 ttcgctgggc acgcacaagg cgcttgggct ggcaaaaaac aagcgggcac gttttctgct
     4081 cgcatctact tctgaaatct acggcgatcc attggtccat ccccagaagg aggactactg
     4141 gggcaatgta aacacaatag gaccgcgtgg ttgttacgat gaagcgaaac gttttgccga
     4201 ggcaatggca atggcttatc accgcgagca tgcggtagaa acacgcattg tgcgcatttt
     4261 taacacatac gggccgagga tgcggttgaa cgacgggcgg gtggtcccgg cgtttgtcag
     4321 ccaggcgttg aagaacaaac cgatcacggt gttcggcgag gggaagcaga cgcggagctt
     4381 ttgttacgtg tcggacctga tcgaggggat ttaccggctg atgatgagtt cgatggtcga
     4441 gccggtgaac atcggcaatc cgcatgagtt gacgatgctg gagtttgccg atgagatcat
     4501 ccgggcgatc gggtcgcgca gcaggatcgt ccacaagccg ctgccgcagg acgacccgaa
     4561 gcagcggcgg ccggacatca cgcgggcgag gacattgttg aagtgggaac cgaaagtttc
     4621 acttgaggaa ggtttgaaaa agaccatcga atattttcgc ggcaaaattt gagaaccatg
     4681 acaataaaat ttccagcatc gaatttcaga agcgctcccg acactcaact ggacttggta
     4741 aaatttgagt gattgtgatt cccgcaacct tgaaagctcc gtttcgcaag aaagtcatcg
     4801 aaaaaaatat tttatgtttg accatgagac cgattttcag taaccaccac ggattatctt
     4861 ctgatcgaca ggttgggcgt ggcggggccg tgcctttgtt gatcggggga aagaaaactg
     4921 aatgttaaat tcaaagtcat ttttgacggt tgatttcggg gcgggcaccc tcaagttggc
     4981 cgagttcgaa gtcaatgaag cgggcggcct gcggctgaga caatacggcc tcaagtcgtt
     5041 gggcgcggaa ggcgcgaagg aggaaacccg cgagagcgtt ttgcaaaagg cgctccagga
     5101 attgctggcg gaaaaaggcg tgcgctcgag ggacgtgaat gtgtgcgcgc ccggttttca
     5161 cgttttctcg aagttcgtga aactcccgcc ggttgatact tccaaggtca cgcaaatcat
     5221 ccagtacgaa gcgcagcaaa atgtaccgtt cccgctttcg gaagtggtgt gggattacca
     5281 gattttgggt tcgacggcga ccggcgaatt ggaagtgttg ctggtggcga tcaagtcgga
     5341 catcgtcgag ggattgttcc gcgtgagcga agcggccggg ttgcggttgc aactggcgga
     5401 tgtttcgccc gcggcgttgt gcaacgcgtt ccgctataat tatggcgatc ttgaagattg
     5461 cacgatgctg ctggacatcg gcgcaaagac gagcaacctg ctgttcttcg agaaggggaa
     5521 agtttattcg cgcagcatca atcttggcgc gaattccatc acgcaagatt tttccaacga
     5581 gtcgaaattg gcgtggggcg aggcggaaaa gaaaaagatc agcgaagggt ttgtaagcct
     5641 gggcggcgcg tacgaagagc cggagaaccc gcatcaggcg gcgatctcga agatcgcgcg
     5701 gcagttcatg acgcggttgc acatccaggt gaaccagacg atgcagtttt atcgcgggca
     5761 gcagggcggt tctgcgccgg tgcgtttgtt cctttgcggc ggtgcgtcgc tgatgccgta
     5821 cacggcgcaa ttttttgcgg agaaattgaa tgtgccggtg gaatatttca atccattccg
     5881 caacgtgcag atcgatccgt ccatcaacct tgaagaactg gcgcgcgtgg cgcattcgct
     5941 cggggaaatt gtggggttgg gattgcgcaa cctggcgcat tgcccggtgg aattgaactt
     6001 gatgcccgag agcacgctga actgggtgca gttcaaccaa aagaagccgt atttcatcgc
     6061 gacggtgttc agccttgtga tagtggtctt tttgtcgagc gtggtgttca gcaaacttgc
     6121 cggcatcaac caggaggaac tggacaagct caacgagaag atccagccgc tgaagacgca
     6181 ggaagttgcc ttcaataatg cgttgcaaga ccgaaagaag gcgaaggacg tcgccgacca
     6241 atacgccgga tggatacaag accggtatta ttggcccgat gtgctgactg aactgcacca
     6301 gttgatgatc caggtggaag ccggcacggc agccaagctt cacaccgaca cgggtgtgtg
     6361 gattgaaaag ttcatcacca cttccacgtc agatgaggcc gcaaacgcga acactcccgg
     6421 ggttccgccg ccaacgtatg attcgaatct tcccggagga agacgggcgc cgttgcgccg
     6481 gcttggtgaa ggcccgccga tggcaacgcc gggcatgccc gccgcttctt ctgccaagtc
     6541 aaccaatgaa gtgtcggtga tcacggtgtt gttccggggc gtcaatctga gcggcgtctc
     6601 acccaccgcc aattcggaca tcgcctatgc gacggaagcg gcgctgcaag cttccccatt
     6661 tttcctcaag accgggacac atttgtcggg cacaattgat caacctggcg acagcgggac
     6721 gttcacgttc gggatggaga tccaattgaa aaccccaatc aagctctagt atgacgtgga
     6781 tcaaacgtaa tttgttattt gtgatcgggg gcgtcatagc cgtggccttg atgggcgcgg
     6841 gaggatatta tctttggaca caaatttctt tggaaggcag cctggcggga cagattgccg
     6901 atcagtacca tgaacttgac cgcttgatcg gcctcaaccc gcatccgggt tacaaggaca
     6961 tcgacaacac caaggcggcg agggcggaga cagcgagcat caaggcttac atcggcaaaa
     7021 caacacctta tttccagcgc atcgcgccga ttcccgacat gggcggcagc aacaagatca
     7081 gcaattcgga ttttgcggcg gcattgcgcg tcaccgtgaa cgagttgaac cattccgcgg
     7141 aacagcagag cgtggaagtg cccaaggact attacttcac attcgaggcg cagagaaaga
     7201 tcatgatttt tgacccggcg agcctcgaca aactggcggt gcaattgggc gaggtcaagg
     7261 ccatcagcga aatacttttt tcggcgcgcg tcaattcgct ggattacatc cggcgtgaaa
     7321 tcgtctcgac tgacgacaag aaccaatcgg attatctgct cacgaaaaca gtgtccactc
     7381 ccatggctga cttgacgccc tacgaagtga gatttcgttg tttcagcgcg gaactggcaa
     7441 ttgtcatggc gaaccttgcg agttccccct acgggttcat cgtgaaggcc atcaatgtcg
     7501 agccgacttc cggcgcggag gaaatgcccg gaggcggcct aatgccaaat gggaatccct
     7561 cgccacaaaa cccaaatcca taccgttaca tgcagaacga cggccggtcc cgggcaccgg
     7621 tcaggtcttt gggtggaccg ccaaccgcca tgcctgttac gcagtcggtt gccgctccga
     7681 cgtcaggcaa gccgcaggcg tttctcactg aaaaaccgtt caacgtgacc ctgctcatcc
     7741 aggtggtgaa gaccaagccg ctacccgcaa aataaggtta atcataatgg agtttcttaa
     7801 aaagaattac gagaaggtgc tgctggccgt ggtgttgctg ggcctgacaa ttgccgcgtg
     7861 cctcctgcca ttcgtcatct cttccaagcg cgctgcgatg gagaacatgg tttcacagcg
     7921 gatcccgcat cccaagccgt tgccgcctct ggacatgacg ctggaagatg ccgcattgca
     7981 gcgcgaacaa acggcttttg cgttggactt cacgacgaag cacaacctct tcaacccggt
     8041 gatttggaaa agaatcgcag gcggaggctt gctcaaggaa tcgacgggca atgaagaagg
     8101 cgcggcggcg ttggaagtca ccaacatcaa agcgctttac ctggaactca agtttggttc
     8161 gcccagcgcg agcggttata tgattgatat cacccggcag ggcgcgccgc gcgaggacct
     8221 tcgccacaag tcgagctaca tcagcaagga aagcaagacc gacctgctca cgctgcttga
     8281 ggtgaaaggc gataccaaca aaccatcgga actcgtgctc gaatggaacg aaagcggcga
     8341 gatgaagggc gacaccatct ccatcgggcc ggacaaaccg ttccgcaaaa tcgaggccta
     8401 ctcggcggat ttgaaatatc cgccggaaaa aagagaatgg accgaccgtc gcgtaggctt
     8461 ccgtttcagc atcgccaatg cttactacaa aatcgttgcc atcactccaa caaacgttgt
     8521 agtgttggcg gaatcaaaca ataaaaagac gaccattact tttcatcctg caaccgaacc
     8581 gcgttgaagt tcttcaacaa acgccacaca acccatgatt aaagcctcaa aattgttttg
     8641 tttgattttc cttgccgctg cggcgacaag tctccaagcc caacccgtca gcgacccggt
     8701 gcaggccggc attgatgaag cgatccaccg ccaggccgac accatcgcct tgcgcaggac
     8761 gttggtggaa gccatggaag cgcgccagcg cggcgacctt ctcggcgcgc acaagcttta
     8821 cgaacgctgc tatgattaca tgcagcgcat cgggcccggc ggcgtcgaac ccgaagcgca
     8881 ggcgaccatc gccggcttgt ccgaagtgct gatgaccatg gcgcgcgccg accaaaagcg
     8941 ccaagattac aaagaggcgg atgtgaagct gacgcgcgtc ctggtggttg acccgaaaaa
     9001 ccaggtggcg cgggacgcca agcgcgacaa tcaaaggatg ctacagaacc aggccggcac
     9061 cattcccagc caggagacga tcgagcgcat tcccgaattt cggaccaatc aggtccagat
     9121 cggcacgttg atccaggatg gcaagctgtt gttcgaagcc gggcatcttg atgaagccga
     9181 ggcgaagctc aagctcgctt atgcgcagga cccttccagt gttgcggctt atgaatatct
     9241 ggcggccatc caacagcggc gcatcgccgg cgcatcgcgc gacacggaat tggaagcaaa
     9301 ctcaagcctg cttcacgttg aaaaggcgtg gacgaaagac cgcagcaatg gcgactatgt
     9361 tccgcggccc aatccattta atcgaaccaa tctggtttat acgggcaagg gacggcaact
     9421 catcctctcc aagctggacc acatccatat tgattccgtg aaatatgacg acctgccgtt
     9481 gacggaagtc atcaataact tgagcgaatt ggcgcgccaa cgcgatcccg acaaactcgg
     9541 cattaatttc tacattgacc gccagactcc cgccgcgatc ggcgctgctt ccggcgcgat
     9601 tgatcctgcc acgggattgc ctgtggcggc gccgctcccc acggacgtgg gcgacatcac
     9661 atcggtcacg gtgaagatca ccccgtcgct caccgatgtc cggttggcgg atgtgctgga
     9721 tgcgatcacc aagactgcaa gcaaaccgat ccgttattcc atccttgatt acgcggtgag
     9781 cttctcgctc aagggacaag aaccggtgcc tcttgaaacg cggtggttcc gtgttgaccc
     9841 taatacgttc cgcatgggct tggaaagcgt tggcggcatt gaatttggaa atatcacgac
     9901 gtcgtccggc agtggtggtg gtggcggcgg cggtggcggc ggtggtggcg gtggtggcgg
     9961 cagcggtagc acaaccacgg caatattgcc acaagtcaac gtcactggca gcacgaccac
    10021 cggtggcggc ggtggtggtg gtggcggcgg gcaaagcggt ggcggacaaa gcggcggtgg
    10081 tcttcgctat gtcaccagcc gcaccaatga gaccgcgctc ctgcaggtga tggtgcgcca
    10141 gttcttcaac tcggtcggat tggatttcaa ctcgaacaat cccgccaata tcggcaaaac
    10201 cttcgtattc aatgaccgca aggggattct ggtcgtgcgt tcgaccgcgc aggatttgga
    10261 catgatcgat gcggccatcc aggcgcttaa ccaagcccct ccggaagtga acatcaaggc
    10321 caagttcatt gagatcacgc aaaacgacaa tcgcgggctt ggtttccaat ggacgcttgg
    10381 gaacacccag atcggcagcg cggcggttgc ttccggcgga acgcaaccga gcttgaacgg
    10441 aacgtccacc acagtaaatc cggaaggcac gtttcccggt tcttcgattt tcggcaccac
    10501 cactccgccc agcgggacgg acggtgtgct gacaagcggg ttgcgcaaca cctttggttc
    10561 cgccgggacc actctgccaa ccgttggcac tttgacgggc atcctgaccg atcctcaatt
    10621 tcgagtggcc atcaatgcct tggaacagcg cgatggcgtg gatgaattga atgcgccgga
    10681 agtcacgact gagagcggtc gtcaggcgca gattcaagct gtggacgtcc agaccatcgt
    10741 gaccggaaat caagtgactg ccagcacggg ttcttctgtc tcgggcgttg ccaacggcat
    10801 caacagtccg attgtccaag cggtgccgac gatcaattat tcgacatctc ccattccatt
    10861 cggcccggtg ctggacgtgg ttccgtatgt ttccgccgac gaattttcgg tgcagatgac
    10921 gttgattccg accatcacgg aatttgtggg atacgacagt cccggcaatt tcgtgccgga
    10981 ggcgttagta tcgggcccga acgcgcaagc ggcctcgata caaggggcgc tgccattgcc
    11041 gcacttccgg atgcggcagg tgacgaccag cgtgacggtg tgggattccc agacggtggt
    11101 gctgggcggg ttgttgaccg acagcgtcac caagatcaag gacaaggtcc cgatgctggg
    11161 agatcttccg ctggttggcc ggttgttcca gagccagtca tcgagcaaga ccaagaagaa
    11221 cctgatgatt tttgtcacgc cgaccatcat caacccggat ggcacgcgct ttcactcgga
    11281 cgaggaaatg ccgtttgcgc agggcggcgt gcctgtgcaa caacctgtca tccgctaaac
    11341 ttggaggctt gaattcggaa tccttttaac tgcggccaaa acaagagcag tcttggattt
    11401 gaactcgaac aacaacgcga gccgggagcg gcgaaatcaa tagatggttg tttcacctat
    11461 gaataaaaac ctgagcggac aaaaaaatgc gccttttttt cctcggcaaa cgcattttgt
    11521 cgcaagaaag catggacaac acaagccgcg cttgttacaa aatggtgcaa atcttatgtc
    11581 acaactgcag cgcgctttta aaacgctggg aggattagtt cttcttgtcg cctttccttt
    11641 tgcaattttt ggccagacaa acaacagcac gaatttttac gcggcgcagg ccggtgaata
    11701 cgtccctgca ggcaatctgc cgggcgacca gacgcattcc tcgctcgcct tgaatgcgtc
    11761 gggcggctat ctggtgtggc aggacaacat caccgacggc gatggcttgg gcatcagcgc
    11821 ggtgcggctg gacagcacgt tttccccgac gctcggaaat tttcgcgtta accagcaggg
    11881 cgccgcggac caggaaaatc cgcaggtggc gctgttgaat ggcggcggcg cggtgtttgt
    11941 ctggcaaagc gggaagcaga gtttccaaca tatcatggcg cgtttcctcg gctccaatgg
    12001 tctttggacc acgggcgatg tgcaggtcaa cacggcgaca aattatcaag tcaatcccgc
    12061 ggtggccgtc ctcaccaacg gcaacgtgat cgtgacttgg ggcagttacg gcgaggacaa
    12121 tgccgacggt cttcagggag tttacgggca gatgctttcc ccgaccggcc agaaagtcgg
    12181 cacggaattc aggatcaacc agttcactcc ttataaccag cgcacaccgg ctgtggcggc
    12241 gctgccgaac ggcaattttt tggtggcatg ggtttcggag cttgaacgca cagttcagac
    12301 atcgttcgac cacatcccaa gcggcatggc ggctttctca gtggacattt atgcgcggca
    12361 gttcaattcg gccggcaccg cgcagggcgg agaatttctc gtgaacgtgc tgaccaacat
    12421 ctgcgcgaac ccggccatcg gagttggctc ggacggaacc tattttatcg catggtcgca
    12481 gaaagattcc gtgacggtga acaacagttg ggacgtgtgg ggccggcagt tcagcaagat
    12541 cggggttggc ggcgcgccgg cgctgatcaa cacacaggtt tacggcgatc aatacgctcc
    12601 caaggtcagt tccatcggca ctgattattt ggtggtttgg accagcatgg gccaggacgg
    12661 ctcgcgcgaa ggtgtttacg gacaatttct gcacggcaac ggcagtcacg tcggcggcga
    12721 attgcgggtg aacaccaccg tgctgaacca gcagatgttt cccgctgttg cctcggatgg
    12781 ggtggggcgg tttatggttt cgtggtccag ctatgtgggt ggaatagaca gtctcgattt
    12841 aaatgcgcaa cgatacgcga cgacgcagac gccgttgacg gcccccggcg cgccgatcgt
    12901 gctggccttg gattcgtaca gcttgagtgt cacctggccg ccagtcgccg gtttcagtgt
    12961 gagttactac gagttgttcg tggatggttc gagcacgccg atcaatgtca ccaacaatat
    13021 gtggtcgtac ttgaattata atccgtcgag cacccacaca ttccaactgg cttatgtgtt
    13081 gacgaacgga aatacatcgc cgctttcggc gacggcgacc ggcacgactt ggggagtgga
    13141 caggaatttt gacgggctgc ctgatgattg ggaggcgaag tattacggaa cgaaccaggc
    13201 caactggccg aagggcggag catccaccgt gctcgcgccg ggcgtgacgc tgctggatgt
    13261 ctttttgtcg ggcgcgaatc ccagcgaccc ctcaacttgg ttgaaagaga cgatcaccaa
    13321 gacttcggag ggttatttcc tgaattggaa cacgattcca ggtgggattt accaggtgcg
    13381 gggttccacc gatttgcaga catggacgaa tctcggttcc caacgttttg aggctggcaa
    13441 cacggattcg atctatttgg gactgtcaac caaaggctat taccaagtct tgagaaaccg
    13501 ttactaagtt ttatatgttc catttcatca aaagattctg ggtggtactg gctttgacgc
    13561 tggcaggggc gcagacaatg tttgggtttg cgatgttggg gccgttcaac gaaggcttcc
    13621 aagtccctgc aatcggctat aatttgacgc ttccggcggc ttcatttggg aatgatggaa
    13681 acaacgccga cgtcggcgcg ccgaagaact acgggcaagg ctatcgttgg aacacgccga
    13741 ccaattatta tgcctttgac gaaagcttct cccgttattt cggcgcgact ggcgaatcga
    13801 acatcgatgc ggctttcgcc atgttcaata atctcaccaa cgtttccaat ctgaaccttg
    13861 atcaatggcc cttgaatgcg agccgcatca attaccgcgc ccaggcgctg caattgctgg
    13921 atttaaaatc ttatacgatg tccataatca tcgagcaact cggcctggcc cagccggacc
    13981 ggtgggtttg gtgcttgcat gatcgtttta ctggcggggc cacctgcccg ggtttcgaat
    14041 attcggtcat ccaacgcaac tacgacccgt acacctatct ctacagttcc tatgtcaacg
    14101 gtgtgtttta tgactatcaa atcgaggagc tttgtgcgct gactcccttg aatccatttg
    14161 cgcccttgct ggccgatgcg atagaaatcc cggtggatcc caccacatac caggacacga
    14221 ccgacgccgt ggcggcttat gggtcagagt acatctatgg atcatttttc ctcagcttta
    14281 cgagggacga catcggaggg ttgaaatgcc tttattccgc gacgaatgcg tatccggaga
    14341 tggctgaatc gaacagcgtt caaatcgtga ccaataccgc gcagccggtg ctgctggtca
    14401 cctccaacct ggcgattttt gaatcgcagg cattgaccaa tgatgacgc
//
LOCUS       DASZMM010000048         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3042687, whole genome shotgun sequence.
ACCESSION   DASZMM010000048 DASZMM010000000
VERSION     DASZMM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3042687"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..530
                     /locus_tag="VG754_01190"
     CDS             225..530
                     /locus_tag="VG754_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HWF17848.1"
                     /translation="MKSAHSYQVSGGASVSGKSLAPLVISVYPAGMALTNMAIAEEAL
                     SLSPDEQLALVKLLIQSLEADPRTDAEIKTELKTRLDDLLSGRDKGLTFAEVFGHSS"
     gene            527..829
                     /locus_tag="VG754_01195"
     CDS             527..829
                     /locus_tag="VG754_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17849.1"
                     /translation="MKAVFSSRFKADLGAEEAKYLLISDRLASAFKERVAGQAREIVK
                     WNGGDHVGPHGFPCRRATPFPFYICYSVVADTIYFLGLVHERRHPSFLKNRLAKGK"
     gene            1543..1680
                     /gene="rpmH"
                     /locus_tag="VG754_01200"
     CDS             1543..1680
                     /gene="rpmH"
                     /locus_tag="VG754_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWF17850.1"
                     /translation="MKRQYQPSKIRRKRQHGFLNRNSTKSGRAILASRRRNGRKRLTP
                     V"
     gene            1695..2066
                     /gene="rnpA"
                     /locus_tag="VG754_01205"
     CDS             1695..2066
                     /gene="rnpA"
                     /locus_tag="VG754_01205"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWF17851.1"
                     /translation="MAGEPKPLRFGREMRLKQSRDFARVRQEGRRMVCGCFIANWRVL
                     PPGSGMKLGVITAKKLGGAVVRTRARRLLREVFRLHQHDFLQPVELVLVAQRTIVDMG
                     LRDVEEAFLTMLRKGRLLKTE"
     gene            2072..2392
                     /gene="yidD"
                     /locus_tag="VG754_01210"
     CDS             2072..2392
                     /gene="yidD"
                     /locus_tag="VG754_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWF17852.1"
                     /translation="MQQSLIFALRCYQRVVSPMLMGLFGPLGRCRFEPSCSQYAIEAV
                     RVHGAIKGALLAVWRLCRCHPWGGCGVDQVPKKFKVQSLRFKVGEKVDGCACGEGHVT
                     EGQG"
     gene            2411..4243
                     /gene="yidC"
                     /locus_tag="VG754_01215"
     CDS             2411..4243
                     /gene="yidC"
                     /locus_tag="VG754_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWF17853.1"
                     /translation="MDRKSIIILVLCGVLIVFWPALMRRLYPPAPVSTNQVAAVTGTN
                     TTYTNNATNAIVTSTSITNTSSTFVVNSSAPEQLLVVTNTNARYTFTSRGGGLQLVEL
                     VGYPETVSTVRRKKGQTNQWATLNAPTMPPLLAVLGDESLQGDNIYKLSEITNGVRAE
                     KSLPNGLTIVKDFQLGTNYLVSASVRLENHSRRELTIPPYQWSAGTAAPMSPQDNGMA
                     EMVQWFNGSKAASVALSYFNTNTSSFFGLMSRTPHTEYVEGSNNVVWSSAQNQFFVIA
                     TMPDKPAQAVVVHMVDLPPPTEDELKDSPNAIRNPHGLESALIYPGLKLAPGGVAASQ
                     FIVFAGPKEYQTLAALAARFNNEIDRVMNFGWSGPISKVLLVAMNWIHHSLGLPYGWT
                     IIFITIFLKVVFWPLTQASTRSAKRMQELQPQLKALQEKYKDDPQKLPAKQWEFYKKN
                     NINPMAGCLPMLLQVPVFIGFYGMLRTAIELRGAPFLWIGDLSKPDTVLSLHLPLHYL
                     GFSDLFFPVNPMPLIMGATLLWQARSAPVSPGMDPSQQKMMKYMPLMMLVFMYNLSSG
                     LAVYWTVNNMLTIVQTKLTKTRAQTAPAVPAKAPASLAPQKKKK"
     gene            4311..4760
                     /locus_tag="VG754_01220"
     CDS             4311..4760
                     /locus_tag="VG754_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HWF17854.1"
                     /translation="MAAQPKDTLEKMLQLLGFDGKVEEHPMEEGYLLDVKTEDSGRLI
                     GRQGQTLSDLQYITNRLLFQQDQSAPKVMVDVGGYRAQAREALVKKAKEAAEKVRRWG
                     DVVELEPLSAFDRRIVHNAIKEDPDIETHSVEVEGTDKKVILFRPRR"
BASE COUNT         1102 a         1245 c         1417 g         1087 t
ORIGIN      
        1 ataaggtgtg taatctgcca ccaaagtctg ccacagttca gattggttcg cgacattgat
       61 agaatgcaga ttactaacgg tggcttgacc ccacgcccct aaaggctgca gcagaaaagc
      121 caacaggcag gttactgccg caagtgattg cgtgagtctc attgctatac gacaaaagtt
      181 attgcacgaa tagggaacag agcgcaagcg cgggatgcac gcatttgaag agtgctcatt
      241 catatcaggt ttctggcggt gcctcagtat cgggcaaaag ccttgcgcct ctggtaatct
      301 cagtttaccc tgctggcatg gcgctaacaa acatggccat tgctgaagaa gctttgtcgc
      361 tgtcaccaga tgagcagttg gcattggtca aattgctgat tcaaagcctc gaagcagacc
      421 ccaggacaga tgcggaaatc aaaacagaat tgaaaacccg gctggatgac cttttgtccg
      481 ggcgggacaa aggattgact tttgcggagg tgtttgggca ttcgtcgtga aggctgtttt
      541 cagcagccga ttcaaggccg atctgggggc tgaggaggcg aaatatctgt tgatttcaga
      601 tcggttggcc agcgcattta aagaacgagt cgcggggcaa gcacgagaaa ttgtgaaatg
      661 gaacggcggc gaccatgttg gcccacatgg ctttccttgc cggcgggcga caccgtttcc
      721 attctatatc tgctactcgg ttgttgccga tacgatttac tttttgggat tggtccacga
      781 acgacgtcat cccagtttcc tgaaaaatcg gctcgccaag ggaaaataat cggtccgccg
      841 cctgaattga tttctccatg tttcatccgt ggctgaattg aatggtcagg gtttggaatc
      901 catccagtcc tttcaggatg gtcgcgactg tgccctttgc aaggcatgcc cagccaaaat
      961 tctatggaca acccgtcaac ctgtgagata atcgccgttg atgcgacgag gagcgcaaca
     1021 gaggacaccg tcaggtgttt gtcgtcaaac ggccatctct tcgccgggaa ggaccgcttt
     1081 tgccctaaat ccatcatttg cgtggcggcg ttccgttccg ggtcctgaat tccgcattcg
     1141 gagttggttg aaatccaaca acaagcgtcg aattcaacag tccacgcaag cggttttccc
     1201 ggtttttacc gcctttttac gccttttacc gcctattttt gaggggggag gggggtctcc
     1261 atagttccaa gttttatgtt caaagttcaa agttgcgttg cgcacgccga acacattgtg
     1321 ccaggccgga cgccagcgct ccgcctttta tgcttaacac accatggttc ttaccgagca
     1381 acaaaccaac atcgaatgcc gaacgtcgaa cattccgccg cttgacagtg aatctttcat
     1441 tggtatcctc tcgcgttccg tgtgaagcgg agtttgccga tggcgcagat gcccacgata
     1501 ttgggcgaag agcgtgtcag tcgggattta attttttgaa ttatgaaacg tcaatatcaa
     1561 ccgtcgaaga ttcgccgcaa gcggcaacac ggatttttga accgcaattc caccaagagc
     1621 gggcgggcca tcctggccag ccgccgccgc aacggccgca agcgcctgac tccggtctaa
     1681 cacaaccggg cggcatggcc ggcgaaccca agcccctccg cttcgggcgt gaaatgcgcc
     1741 tgaaacaatc gcgggatttt gcgcgggtgc gccaggaggg acggcggatg gtttgcggct
     1801 gtttcatcgc caattggcgg gtgcttcccc cgggttccgg aatgaaattg ggcgtcatca
     1861 ccgcaaaaaa attgggcggg gccgtcgttc gcacgcgcgc gaggcggttg ttgcgggaag
     1921 tgttccggct gcaccagcat gatttcctcc agccggtgga gcttgtgttg gtggcgcaac
     1981 gaactattgt ggatatgggc ctgcgggacg tggaagaagc cttcctgacg atgctccgaa
     2041 agggccggtt gctgaaaact gagtgaactg ggtgcaacaa agtttgattt tcgcgctgcg
     2101 ctgctatcag cgggtggttt ctccaatgct gatggggctg ttcggcccgt tggggcgctg
     2161 ccgtttcgag ccgagctgct cgcaatacgc gattgaagcc gtgcgcgtcc atggcgcgat
     2221 aaaaggggcg ctgctggcgg tctggcgttt gtgccgttgc catccttggg gcggttgcgg
     2281 ggtggaccaa gttccaaaaa agtttaaagt tcaaagttta aggttcaaag ttggggaaaa
     2341 ggtggatgga tgcgcttgtg gcgaaggcca cgtcacggag gggcaagggt aacagccggt
     2401 caaggttttc atggatcgca aatcaatcat cattctcgtt ttgtgcggcg ttctgatcgt
     2461 cttttggccg gcattgatga ggaggttata tccgcccgcg ccggtttcca cgaaccaggt
     2521 ggctgctgta actggaacga acacgacata tacgaacaat gcaacgaatg cgatcgtaac
     2581 ttccacaagc atcaccaaca cgagttccac gttcgtggtg aattcttccg cgccggaaca
     2641 gttgctggtg gtgaccaaca ccaatgcgcg ttacacgttc acttcgcgcg gcggcggttt
     2701 gcaactggtt gaattggtcg gttatcccga aacggtcagc acggtccgtc gaaagaaagg
     2761 ccagaccaat caatgggcca ccttgaacgc gccgaccatg ccgccgttgt tggcggtgtt
     2821 gggagacgag tcgttgcagg gcgataacat ttacaaattg tccgaaatca cgaatggcgt
     2881 tcgcgccgag aaatcgctcc cgaacggttt gaccatcgtc aaagattttc agcttggcac
     2941 gaattatctc gtcagcgcgt ccgtccggct cgagaaccat tcgagacggg agttgacgat
     3001 tccgccttat caatggtcag ccgggaccgc cgcgccgatg agcccgcagg acaacggcat
     3061 ggccgagatg gtgcaatggt tcaacggctc gaaagcggca tcggtcgcgc tcagttattt
     3121 caacaccaac accagctcgt ttttcggcct gatgtcccgc acaccgcaca cggaatatgt
     3181 agaaggcagc aacaacgtgg tctggtcctc cgcgcaaaac cagtttttcg tcatcgcaac
     3241 gatgccggac aagcccgcgc aagcagtggt tgttcacatg gtcgatttgc ctccgccaac
     3301 ggaagatgaa ctcaaggaca gccccaacgc catccgcaat ccgcatggat tggaatcggc
     3361 cctgatttat cccggcttga aacttgcgcc cggcggcgtg gcggcaagcc agttcattgt
     3421 gtttgccggg ccgaaggaat accagacgtt ggcggcgctg gccgcgcggt tcaacaacga
     3481 gattgatcgc gtgatgaatt tcgggtggtc ggggccgata tccaaggtgc tgctggtggc
     3541 aatgaattgg attcatcaca gcctggggct gccatacggc tggaccatta ttttcatcac
     3601 gatttttctc aaggttgtgt tctggccgtt gacgcaggcg agcacgcgct cggccaagcg
     3661 gatgcaggaa ttgcagccgc aactcaaggc gttgcaggaa aaatacaagg acgacccgca
     3721 aaaacttccg gccaagcaat gggaatttta caagaagaac aacatcaatc cgatggcggg
     3781 atgtctgccg atgttgttgc aagtgccggt gttcatcggg ttttacggca tgttgcggac
     3841 ggcgattgaa ttgcgtggcg cgccgttttt gtggattggc gacctgtcaa agccggacac
     3901 ggttttgtct ctgcatctcc cgctgcatta ccttggtttc agcgatctgt ttttcccggt
     3961 caacccgatg cctctcatca tgggcgcgac cttgttgtgg caggcgcggt cagcgccggt
     4021 atcaccgggc atggacccgt cgcagcagaa gatgatgaaa tacatgccgc tgatgatgct
     4081 ggttttcatg tataatttgt catcgggcct ggcggtttac tggactgtga acaacatgct
     4141 gacgattgtg cagacgaaat tgaccaagac gcgtgcgcaa accgcaccgg cggtcccggc
     4201 aaaagcgccg gcttcgttgg cgccgcagaa aaaaaagaag tgattgaccg gatgcgcggg
     4261 cctgttcaag actgacggaa gcgcgcaagt taactgaacc gaatcgaatt atggctgccc
     4321 aacccaaaga caccctggaa aaaatgctgc aacttctcgg cttcgacggc aaggtcgagg
     4381 aacatccgat ggaagaaggc tatttgctgg atgtgaaaac ggaggattcg gggcggttga
     4441 tcggccgcca ggggcagacg ctttccgatc tgcaatacat cacgaaccgg ttgctctttc
     4501 agcaggacca gagcgctccc aaggtgatgg tcgatgtggg cggttaccgc gcacaggcgc
     4561 gcgaagcgct ggtcaagaag gcgaaggaag ccgccgagaa agtccgtcgt tggggcgacg
     4621 tggtggagtt ggaaccgttg agcgctttcg accgtcgcat tgttcacaac gcaatcaagg
     4681 aagatccgga catcgagacg cacagtgttg aagtggaagg gacggacaaa aaagtcatcc
     4741 tgttccgccc gcgacgttaa aaatcatcaa tgcgcttgcg gtgaggaatt tgcaaaagaa
     4801 gagggcctca cttacccgcg aggcccactg gcagatgtgc attgatgata t
//
LOCUS       DASZMM010000049         5254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4959622, whole genome shotgun sequence.
ACCESSION   DASZMM010000049 DASZMM010000000
VERSION     DASZMM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4959622"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VG754_01225"
     CDS             <1..210
                     /locus_tag="VG754_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWF17855.1"
                     /translation="GFVDTFREFEKGGGHYTWWSPFSGARKRNVGWRIDYFLISAALR
                     PRLKRAFIHCEVEGSDHCPVGIEIE"
     gene            334..573
                     /locus_tag="VG754_01230"
     CDS             334..573
                     /locus_tag="VG754_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17856.1"
                     /translation="MVTEVQQRFPEGKIAKAIESQTAKMPSDLFLWAAGGAILTSLVL
                     KATKRNHDALFIGQWAPTLLLLGVYNKLVKLHGSD"
     gene            931..1701
                     /locus_tag="VG754_01235"
     CDS             931..1701
                     /locus_tag="VG754_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF17857.1"
                     /translation="MNTSESFRSVNNEVRTSAFTLIELLVVIAIIAILAGLLLPALAR
                     AKLKAQRVACFSNLRQLQVAYKNYADDNQDRLALMIDGGGGDGLPGSWVIGDAQYSAD
                     MTNITSGTLYLYVPNTGVFHCPTDHSTLLTSSTPKNRSYSVSEFLNFLDSSLPDPVTK
                     YDQILPDTSRVFVFIDEDARSIDDGVFCGDRAPSQVWANLPADRHEKGCNLSFADGHC
                     EYWKWLNPKVFIYPGQPVANGNDLKDLQRLQLDFPTPP"
     gene            1857..2615
                     /locus_tag="VG754_01240"
     CDS             1857..2615
                     /locus_tag="VG754_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17858.1"
                     /translation="MIAFVHLVRARNGIEPLRRFLDSYTEHPSNVAHKTLIVFKGFER
                     KVHKPLLEDCRALLKSFDPAVGFVRDWMMLDLDAYGYALRRWRFDYYVFVNSFSIIRA
                     PGWMDKLYAAITLPGVGLASCTGSFESRHGNRLLNRLLFPPFPNPHIRTNAFIISRDL
                     MLKVWPRGIFFKPMSYLFESGRWGLTSRVEKLGFRIVLVGNEGIYEKDQWPSANLFRS
                     EDMSNLLVSDNQTHKYAASSGEEKACLRKMAWGI"
     gene            2988..3605
                     /locus_tag="VG754_01245"
     CDS             2988..3605
                     /locus_tag="VG754_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17859.1"
                     /translation="MQNHHGNPDKTTRVCAIIVTYHPSPALLQNAEAVRQQVSELVVV
                     DNGSDEDSAKLLVAMTSGSLIKTRALIENGLYDEAMFIDYVDFDLCLRLRRRGFKLIR
                     AGRAFLLHCLGTLEAHSFFGFPLALTSHNATRCYYIMRNRVAIYRRYGLLFPFWAFND
                     FIWLLIDFTKIILFEQDKRAKLGNALRGIRHGLAGLSGPMASAAV"
     gene            3610..4521
                     /locus_tag="VG754_01250"
     CDS             3610..4521
                     /locus_tag="VG754_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF17860.1"
                     /translation="MDLEKTNKPPVGVVIVNHNLKESLRETLESFKKVNYPSLKIVVS
                     DNASTDGAQEMVRTQFPDVHLLAHKEEQGYARAASLGMAYLADKTKYIFSTTNDVLVD
                     PEMLNVLVDYAEKNPQAGVLGTRIYFFDRPDVLWHAGGSINPLHGHVRHIGWERKDHP
                     RYGHIRECDYVTGCGYLLRSEVLKKIGFFKEDLVFYSEDAELCYRFREAGCKVMYIPD
                     AKMWHKTGTTLAKNRPVQLHYSTRNQLYLLQRHRVGFYPLSLWIHILIVSPLKMFLFI
                     AIGRRKNAVGIYRGIRDWWRGKYGWIR"
BASE COUNT         1198 a         1329 c         1396 g         1331 t
ORIGIN      
        1 ggttttgttg acacgttccg cgaattcgag aaaggcggcg gccattacac ttggtggagt
       61 ccgttcagcg gcgcgagaaa gaggaatgtt ggctggcgca tcgactattt cctgatttcc
      121 gcggcgctcc ggccccgcct caagcgcgcc ttcatccatt gtgaagtcga gggttcggac
      181 cattgtccgg ttggaataga gattgaatga agccattggg gtgtacaccc gaaaacagtc
      241 ctgtgacctt gcgaaatggc cgggttgagg cattggtact ttgttgaaga attaaatcga
      301 acgacgcgcg agcgcccaat caaaggagac tatatggtca cagaagtcca acaaagattt
      361 cccgagggaa aaattgccaa agccatcgaa agccaaactg cgaaaatgcc gtcagatttg
      421 ttcctctggg cggccggcgg agccatcctg acttcgctag tgttaaaagc caccaagcgc
      481 aatcacgacg cgctgtttat cgggcaatgg gcgccgaccc tgctgttgct gggcgtgtac
      541 aacaagctcg tcaagcttca cggctcggat tagcgacaat tccgcgtaaa atctcgcgta
      601 gttaaaacgt gagattttca gccataccca ttccaaaacc ctctcataat tgccggagcg
      661 cggccgtgcg caagcgccag ccgcagcaca ccgaaattgt gggtgcttca aggagctgcg
      721 actggtcctg cggacacagt cgctctctgt tctttttttt gcgcgaaagt tggacagaat
      781 tttcttgacg ttgtcgtcaa aaagatgaca attcgcgatc aattgtagga ggattctggc
      841 actccgaaga cgagcgcaat ttttttgctt atgtaagcaa gggtgcctgt attgcgcatg
      901 gcaccgtata acaacttggg aaacgatcgc atgaacacgt ccgagagttt tcgctctgtt
      961 aacaatgagg tcagaaccag cgcctttacc ctaatcgaat tgctggtggt gattgccatt
     1021 atcgcgattc tggccggttt gctcctccca gcattagcca gggcaaagct aaaggcgcaa
     1081 cgggtcgcct gctttagcaa tctgcgccag ttgcaagtcg cctacaagaa ctatgcagat
     1141 gacaatcagg acaggcttgc attgatgatt gatgggggcg gtggtgacgg cttgcctggt
     1201 tcctgggtca tcggtgatgc acagtacagc gccgacatga ccaatatcac cagcgggacg
     1261 ctttatcttt acgtgcctaa tactggtgta tttcattgtc ccacggacca ttccaccctc
     1321 ctcacctctt ccacgccaaa aaaccggagt tattcggtca gcgaattcct caatttcctt
     1381 gactcgagtc tgcccgaccc cgtgaccaaa tatgatcaaa tcctgccgga cacctcgcgt
     1441 gtttttgttt tcattgatga agatgccagg agcattgatg atggggtctt ttgcggtgac
     1501 cgcgctcctt cccaagtttg ggccaacctg ccggctgatc ggcatgaaaa gggctgcaat
     1561 ctttccttcg cggatgggca ttgcgaatat tggaaatggc ttaatccaaa agtcttcatc
     1621 tatcccggcc aaccggtggc gaatggaaat gacttgaaag acttgcaacg gctgcagtta
     1681 gactttccaa cacctcctta aactcaggat caggtgacat gggaatttat agtggtcacg
     1741 tgctaacccg caaagcatct tatccccctc caattggtaa aaccttcttg ccttcgcccc
     1801 cctattctta aattattctt ggataagaat tgacccggct gtaaagcagg gaatttatga
     1861 ttgcgtttgt tcatttggtg cgcgcccgca atggcatcga gccattgcgg agattccttg
     1921 attcttatac agagcatccg tccaacgtcg ctcataaaac gctgatagtt ttcaaggggt
     1981 tcgagagaaa ggtgcataaa cctttgctgg aggattgccg agctttatta aaatctttcg
     2041 atccagcggt tggttttgtc cgagactgga tgatgttgga tttggatgcc tatgggtatg
     2101 ctttgcgccg ctggaggttt gattattatg tttttgtaaa ttctttcagc attattcgag
     2161 cacccggctg gatggacaaa ttatatgccg cgatcacgct tccgggtgtg ggattggcca
     2221 gttgcaccgg ctcgtttgaa agccggcatg gcaacagact tttgaaccgg ctcctgtttc
     2281 ctccttttcc caatccgcac attcgcacca atgcattcat catctcccgc gacctcatgt
     2341 tgaaagtatg gccccgcggc atctttttca aacctatgag ttacttgttt gaaagcggtc
     2401 gttgggggtt gacatcgcgt gtggaaaaac tcgggtttcg aatagtgctg gtgggcaatg
     2461 aaggcattta tgagaaggac cagtggcctt cagccaacct cttccggagt gaggacatga
     2521 gtaacttgtt ggtttcggac aatcagacgc acaaatacgc tgcctccagc ggggaagaaa
     2581 aagcatgttt gcgaaaaatg gcttggggaa tctgatggct gaaacggcgg tgaacggttt
     2641 ccagttccaa ccctggcgca gacagccttt cccacggttc aacggatcca cccattggcc
     2701 gcgccagcaa ccgcggattc cgcggtaaat gcctgccgaa ttttccagcg tcgaatcgcc
     2761 aggaacagcg ccttcttgaa cgggtttacc acgaggagat tgaccaaaag gcagagcgta
     2821 aaagttcacc cattataagg ggtcagattc ggccccaatt ccactccgtg cttgccacaa
     2881 aagctgttcc atggttcggt gttggcaaaa aatcggatgc gcacccacat tataactagc
     2941 ttaatattcc agggccgatc ggcttgtctt acgccggcat taaggtgatg caaaaccatc
     3001 atggcaaccc ggacaaaacg acgcgtgttt gcgctatcat tgtcacgtat catccttcgc
     3061 ccgcgttgct ccagaacgcc gaggctgttc gacagcaggt ttcggagctg gtcgttgtgg
     3121 acaacggctc ggatgaagat tccgcaaagc tgctggtcgc gatgacctcc ggcagcctca
     3181 tcaaaacgcg tgccttgatc gagaatggat tgtatgatga agcgatgttc atagattacg
     3241 tggatttcga tctttgcctg cgcctgcgcc gtcgcgggtt caagctgatc cgcgcggggc
     3301 gcgctttttt gctgcattgc cttggcaccc tggaggcgca ttcattcttc ggttttccgc
     3361 tggcgttgac ctcccacaat gcaacgcgtt gttattacat catgcgcaac cgggtcgcga
     3421 tataccgccg ctacggcctg ctgtttccgt tttgggcctt caacgatttc atctggctct
     3481 tgattgactt caccaaaatc atcctgttcg aacaggacaa gcgcgccaag ctcggcaatg
     3541 ccttgagagg catccgccat gggctggccg gcctgtcggg accgatggct tccgccgccg
     3601 tttgaaccta tggatttgga gaaaaccaac aagccacccg tcggcgtggt catcgtcaat
     3661 cataacctga aggagtcgct tcgggaaacg cttgaatcgt tcaagaaggt caattatccc
     3721 tcgctgaaaa ttgtcgtctc cgacaatgcc tcgaccgatg gcgcacagga aatggtgcgc
     3781 acgcaatttc ccgacgttca tttgctggcg cacaaggaag agcaaggtta cgcgcgcgcc
     3841 gccagcctgg gaatggctta tctggcggac aagaccaaat atattttcag caccaccaac
     3901 gacgtgcttg tggatccgga gatgttgaat gtattggtgg attacgcgga aaaaaacccg
     3961 caggcaggcg tgttgggaac gcggatttat ttttttgacc ggccggacgt gctctggcac
     4021 gcaggcggaa gcatcaatcc gcttcatggc cacgtgcggc acatcggctg ggaacgcaag
     4081 gaccatcccc gttacgggca cattcgcgag tgcgattatg tgaccggctg cggctatctg
     4141 ctgcgttccg aggtcttgaa aaaaatcggc ttcttcaagg aagaccttgt gttttattcc
     4201 gaggatgccg agctttgtta tcgcttccgt gaagccggct gcaaggtgat gtacattccc
     4261 gatgccaaga tgtggcacaa gaccggcacg acgctcgcaa aaaaccgccc ggtccaactc
     4321 cactacagca cccgcaacca gctttatctt ttgcaacgcc atcgtgtggg gttttacccg
     4381 ctctcccttt ggattcacat tttaatcgtc tcaccgttga agatgttcct gttcatcgcg
     4441 attggccgca ggaaaaatgc tgtcggcatc tatcgcggca tccgcgattg gtggcgcggc
     4501 aaatatggtt ggatcaggta attgaccgcc gggtagtgtc ctaagaaaat cgccgttatt
     4561 atccagaatc cagccggaca ggttctggag attgattggc atgggcggat tagcaggatt
     4621 ttgtacggaa taaaatgtac caaatgaggg aagcgattcc ggcggtaatg ggtcgatttg
     4681 ttcggcggct tgaatcaggg cggacagatc acccggcgag atggcaaatg cggatgtggc
     4741 gaagagcagg aattccaagg caaacagcag cccccatttt ttgattctca cgtttccttt
     4801 cgattaaaca actgttatgg ctgcggaggg tgcgtcgttt actcaactcg tgcaatagat
     4861 attttgtaga tatttgagga agttgagggc cgcgcggcgt ttccctgctg atacctatgg
     4921 gaggcttgac agcgcgtggt aaaattgtcc gcatgatagt tgaacctgaa aaatggtttg
     4981 gatggtgaag gtaaattccc ctcccggttg caactttgct gtttcccgtc tctgccttga
     5041 tgacggggct gttctttgac attctaaaat cgttccgagt ctcgcaagcc aagtttaacg
     5101 tttaaagttg cggatttcgc ggactttcgt gtttttcgcg ggcaagtcgc gtctcggttc
     5161 tctcatgctt ggacgtttgg cggacttcgc cgaacatggc cctgaaaact cacgttttca
     5221 gctacgagtg attgggcgtt caagttgtaa gtgg
//
LOCUS       DASZMM010000050         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3043094, whole genome shotgun sequence.
ACCESSION   DASZMM010000050 DASZMM010000000
VERSION     DASZMM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3043094"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VG754_01255"
     CDS             complement(<1..651)
                     /locus_tag="VG754_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17861.1"
                     /translation="MIKAYRFFVFACLCGLLAGIAHAETLHLTDGQTITGDVVSMDGT
                     GIILKNADGSYGDRIPWSKLSQDNLKELDQNPKAAQYVEPFIEPSQEEKSRKTEIEIK
                     DYPHLPRPSGHSLAAALFTSPMGLFVLLMIYGANFYAAYEVSIFRAQPVGTVCGVAAV
                     APIIGPIIFLSMPPKLKKKEANWKVAEHQMDAGVAAAIAAEQAVPAEIAAAEAAAAV"
     gene            complement(836..2257)
                     /locus_tag="VG754_01260"
     CDS             complement(836..2257)
                     /locus_tag="VG754_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWF17862.1"
                     /translation="MSVPIISVPQMREWEKATWAAGQTESAVIARVGEAVAQRTLQST
                     SPGDAILILAGKGHNGDDARQAESHLRDRRVLICSVNDPAADLAAVTQQLEESPALIV
                     DGLFGIGINRPLDEKWQALIQRVNDSRIPILAVDVPSGLNAETGEPEGAAIRAAITLT
                     VGAPKKGMLLPPAWPYVGRLEVATDVGLAKCPFTGEINWILPRDFRNFPPARPAVSHK
                     GDYGHLGIFAGSLGYHGAAVLTSRGAQHAQPGLITLHTPETVYHVVASQLQAVMVRIW
                     EPTLKMPDHYGAVLIGPGLAAENLPDAVKMFTRLTWRDSPVPVVVDASALDWVPVAPL
                     PKTAIRVITPHPGEAARLLRTAAQHVQADRSGALREISKRHGNCWVVLKGAQTLIGRS
                     EGPIAVNSSGNPRLAQGGSGDVLSGYLSGLLAQRALQADTFTTLCYGVWQHGAAADRL
                     SAVKPNWVVEDLVVEIGNCQKHV"
     gene            complement(2349..>2614)
                     /locus_tag="VG754_01265"
     CDS             complement(2349..>2614)
                     /locus_tag="VG754_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWF17863.1"
                     /translation="PVRIIVPWKYGYKSIKSVVKIEFIEKQPGTLWETLAPNEYPFES
                     NVNPKVPHPRWSQAMERVIDTGDYVPTQPYNGYGSYVAKLYKS"
BASE COUNT          555 a          751 c          733 g          575 t
ORIGIN      
        1 aactgcggcg gcggcttccg cagcggcaat ctccgcaggg acggcttgtt ccgcggcgat
       61 ggccgccgcg acgccggcgt ccatctggtg ttcggccact ttccaattgg cttccttctt
      121 tttcagcttg ggcggcatgg acaggaaaat gatcggcccg atgattggag ccaccgccgc
      181 gacaccgcaa accgtgccga caggttgggc gcggaaaatt gaaacttcat aagccgcata
      241 aaaatttgcg ccatagatca tcaataacac gaacagtccc atcggcgagg tgaacaatgc
      301 agccgccagc gaatggccgg atggacgcgg caggtgggga taatccttga tttcaatttc
      361 ggttttcctg gacttttcct cctggctcgg ctcgatgaac ggctcgacgt attgcgcggc
      421 cttggggttc tggtccagct ccttgagatt gtcctgcgaa agtttgctcc agggaattct
      481 gtcgccgtag gagccgtcgg cgttttttag aatgatgccg gtgccgtcca tggagacaac
      541 gtcgccggtg atggtctggc catcggtcaa atgcaaagtt tccgcgtgag cgatgccggc
      601 cagcaggccg caaaggcacg caaagacaaa aaatcgataa gctttaatca cggttcattt
      661 ttgcataaca aatgggccgg ggcaaaggaa gaaaatttat ggttggccga cccctcggcc
      721 catgcttttt aaggacgcct aaatggcttc ggagtgggag cgagcgttga aggcgctcat
      781 tcgatttgaa atttcaaatt tgaaatccgg gcagagcggg gttgtctcaa ctccttcaaa
      841 catgtttttg gcaattgcca atttccacga ccaaatcttc aacgacccaa ttaggtttca
      901 cggcactcag cctgtccgcc gcggcgccgt gttgccatac cccgtaacag agcgtggtga
      961 acgtgtccgc ctgcagcgcc cgttgcgcga gtaaaccgga caaatagcct gacagcacat
     1021 caccgctgcc gccttgcgca agacgcgggt tgccggaaga attcacggcg ataggccctt
     1081 cgctgcgccc gatgagcgtt tgcgcccctt tcaacaccac ccagcaattg ccgtggcgtt
     1141 tcgagatttc gcgcaatgcg ccggaccggt cggcttgcac atgctgggcc gctgtccgca
     1201 acaatcgcgc cgcttcgcct ggatgcggtg tgatgacgcg gatggctgtc ttgggcaacg
     1261 gcgccacagg aacccaatcc agcgcgctcg cgtccacgac aaccggcaca ggcgagtcgc
     1321 gccatgtcag gcgcgtgaac atcttcaccg cgtccggcaa attttccgcc gcaagacccg
     1381 gcccgatcaa caccgcgcca taatgatcgg gcattttcag cgtcggttcc catattcgca
     1441 ccatgaccgc ttgcagttgc gacgcgacaa catggtaaac ggtttcagga gtgtgcaacg
     1501 tgatcaagcc aggttgggcg tgttgcgcgc cgcgcgaggt caacaccgcc gcgccatgat
     1561 aaccaagact tccggcgaaa attccaaggt gaccgtaatc gcccttgtgc gaaaccgccg
     1621 gtcgcgccgg ggggaaattc cgaaaatccc gcggcaatat ccagttgatt tcgccggtga
     1681 acggacattt tgccaaaccg acatccgtcg cgacttccaa tcgcccgacg tatggccatg
     1741 ccggcgggag cagcattcct tttttcggcg cgccgaccgt caatgtgatt gccgcgcgaa
     1801 ttgccgcgcc ttcaggttct ccggtctcgg cgttcagacc ggagggaaca tccacggcaa
     1861 ggatgggaat gcgcgaatcg ttgacgcgct ggatcaacgc ctgccatttt tcgtcgagcg
     1921 gacggttgat gccaatgccg aacaatccgt ccacaatcaa tgccgggctt tcttccaatt
     1981 gttgcgtgac ggcagccaaa tcggctgcag gatcgttcac gctgcaaata agcacgcgac
     2041 ggtcgcggag atgcgactcg gcttggcgcg cgtcgtcccc attgtggcct ttgccggcga
     2101 gaatcaagat cgcgtcaccg ggactggtgg actgaagcgt gcgttgcgcg accgcttcgc
     2161 ctacgcgagc aatcaccgcg gattcagtct gacctgctgc ccaagtggcc ttttcccatt
     2221 cgcgcatctg gggtacgcta atgatcggca cggacatgat ggtttgtata tacagctaat
     2281 tcgtttcggt aaaagcaaaa acaacttatt gagtcactat atcgagttac aatcaggcgg
     2341 caaactcttt acgatttgta aagtttcgcc acgtaactcc cgtatccatt atacggctgg
     2401 gtgggcacat aatcacctgt gtcaatcacc cgttccatcg cctgcgacca ccggggatgg
     2461 ggcactttgg ggttaacgtt ggattcaaaa gggtattcgt tgggcgcaag cgtttcccaa
     2521 agcgttccag gctgcttctc gataaattca atttttacga ccgatttgat gcttttgtaa
     2581 ccgtatttcc acggcacgat gatgcgtacc ggcg
//
LOCUS       DASZMM010000051         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1663013, whole genome shotgun sequence.
ACCESSION   DASZMM010000051 DASZMM010000000
VERSION     DASZMM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663013"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VG754_01270"
     CDS             complement(<1..1103)
                     /locus_tag="VG754_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWF17864.1"
                     /translation="MPALKTFSALGCALALAFSVAVHAANVETDTSISGKPIDPDLAI
                     KEFKLPEGFKVDLFAAEPQMMNPVAFTFDEQGRVYVAETFRYMTSVFDIREHMPMYYD
                     DLACRNVGDRASMIHRFLGDQTPLMAKESEVVQLLEDRAGTGRADHSTVFANGFNTIL
                     DGIGAGVLARKGKVYYTDIPNLWLLQDTNHEGKATSRKSLSFGYGVHFGYTGHDLHGL
                     RIGPDGKLYFSIGDRGLNVTNMEGKVLSYPDMGSVLRCNLDGSDLEVFAHGLRNPQDL
                     AFDDYGNLFTGDNNCDHGDAARIVYVVEGGDTGWRIANQFSETTPAGVWNSEKLWELQ
                     FPGQAAYILPPIAHMADGPSGIAHYPGTGFSDA"
     gene            1244..2320
                     /gene="recF"
                     /locus_tag="VG754_01275"
     CDS             1244..2320
                     /gene="recF"
                     /locus_tag="VG754_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HWF17865.1"
                     /translation="MHLASMRLRDFRNYARLDVDFAPGFHVLLGDNAQGKTNVLEAIY
                     LMATLRSFRGVGGAQMIRHGQKGYFVGGKVVGQGTHEIKMYWSASERKLGLDNQPIRK
                     LTDYLGVLRVVIFCTEDLQLIKGPSRARRRFVDLLLSQTHISYLPLLQRYARALRSRN
                     AILKQRQPDEAALDSFSTELIKLGNEIIQRRRELVPKLSPLARLAYRRISNDAEDLRL
                     EYQPSVKKDFAVELAQSSRRERTYRSTLIGPHRDEFTMQLNDRAAAQFGSEGQKRTLA
                     IALKMAQAEYLTGIHGSPPILLIDDVMGELDVKRRSGLLPLLERAHHAKGQVFMTCTE
                     ENWPRELGRDLQRWEIKSGTLKKI"
     gene            2568..2810
                     /locus_tag="VG754_01280"
     CDS             2568..2810
                     /locus_tag="VG754_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17866.1"
                     /translation="MTAAEILQEFKALPISERARVVEQALRQLTAEERKPVERLIRRL
                     QHPEVPESFWEGVEDHEDGRSVEMDIALKDTPPGRQ"
     gene            2948..3073
                     /locus_tag="VG754_01285"
     CDS             2948..3073
                     /locus_tag="VG754_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17867.1"
                     /translation="MSAALGKTVYAVEIEADLRAVFYLEGDVAHTVDIGSHGIYR"
     gene            3127..4092
                     /gene="trpS"
                     /locus_tag="VG754_01290"
     CDS             3127..4092
                     /gene="trpS"
                     /locus_tag="VG754_01290"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWF17868.1"
                     /translation="MRILSGIQPSGALHLGNYFGMMRPAIELQDKGETYYFIADYHSM
                     TSLFDANERRKNTLDVALDFLACGLDPKKSVFFKQSDVPEVTELSWLLSTVTPMGMLE
                     RATSYKDKIARGIAPLHGLFAYPVLMAADILIYDSNVVPVGRDQKQHLEMTRDMAAKF
                     NETYGQTFVIPDAQIREETALVPGIDGQKMSKSYNNTIEIFGEEKATRKKIMGLVMDS
                     RSPSEPKPDADKNLAIQLLKLVAPADVAKDFEDRLRAGGLGYGDLKKALFEHYWNYFA
                     AARAKRAELVANLDHVNSVLADGAAQARNLAQKVLKRAKVASGLD"
     gene            complement(4180..>4671)
                     /locus_tag="VG754_01295"
     CDS             complement(4180..>4671)
                     /locus_tag="VG754_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF17869.1"
                     /translation="IEKHPDFPFILITPQCPEGRKWSDEIVLGILDAVTAKYAVDTNR
                     IYLTGLSMGGYGTWSLATTYPERFAAVAPICGGEGNIGVVLSLADKSKAPALKKLPVW
                     AFHGGKDNVVSPEESKRMIEIFKRAGNADAKLTIYPEATHNSWTETYNNPELYEWFLK
                     HHL"
BASE COUNT         1097 a         1300 c         1266 g         1008 t
ORIGIN      
        1 tacgcatcgg aaaaacctgt gccgggataa tgcgcgatgc cggacggccc gtccgccatg
       61 tgcgcgattg gcggcaggat atacgccgcc tgcccgggaa attgcagttc ccacaacttc
      121 tcggaattcc acacgcccgc aggcgtggtt tcgctgaatt gatttgcgat gcgccagccg
      181 gtgtcgccgc cttccacgac ataaacgatg cgcgcggcgt cgccgtggtc gcaattgtta
      241 tcgccggtga aaagatttcc gtaatcgtcg aacgcgaggt cctgcggatt gcgcaagcca
      301 tgagcgaaca cttccaagtc cgagccatcc agattgcaac gcagcaccga gcccatgtca
      361 gggtagctaa gcaccttgcc ttccatgttc gtgacgttca acccgcggtc gccgatgctg
      421 aaataaagtt ttccatccgg cccgattcgc aatccgtgca aatcatgacc cgtgtagcca
      481 aaatgcacac cgtaaccaaa actcagcgac ttgcggctgg ttgcctttcc ttcgtgattg
      541 gtgtcttgca ggagccagag attgggaatg tccgtgtaat aaacttttcc cttgcgcgcc
      601 agcacgcccg cgccgatgcc atccaaaatg gtgttgaagc cattggcaaa aaccgtcgaa
      661 tggtccgccc ggccggttcc cgcgcggtct tcaagcaatt gcacgacctc ggattccttg
      721 gccatcaatg gcgtttggtc gccgagaaaa cggtggatca tcgaggcgcg gtcaccaacg
      781 ttgcggcagg ccaagtcgtc gtaatacatc ggcatgtgct cgcggatgtc gaaaacactc
      841 gtcatgtaac ggaacgtctc cgcgacatat acccggccct gttcatcgaa agtgaacgcg
      901 acgggattca tcatttgcgg ctcggcggcg aacaaatcca ccttgaaacc ttcgggcagc
      961 ttgaactctt tgatggcaag gtccggatca atcggtttgc cggaaatgga cgtgtcggtt
     1021 tccacgttgg ctgcgtggac agcaacggaa aacgcaagcg ccaacgcgca acccaaagcc
     1081 gaaaatgttt taagagcagg cattttgaca gatgataaac ccggacaacc gggaagcaag
     1141 aaactaaact aatgacaagg ggatgcaagt ggcgttcaag tttcgctcca tcgcgaacca
     1201 caaaaaactc gcgtgaacca cgcaacgata ccatgctgtc accatgcatc tggcttccat
     1261 gcgccttcgc gacttccgca attacgcgcg gttggacgtt gattttgcgc ccggtttcca
     1321 tgtgctgctc ggcgataacg cgcagggcaa gaccaacgtt ctcgaagcca tttatctcat
     1381 ggccacgctg cgctcgtttc gcggcgtcgg cggcgcgcaa atgatccgtc acgggcagaa
     1441 aggttatttc gtcggcggca aagtcgtcgg gcaaggcacg cacgaaatca agatgtattg
     1501 gtccgccagt gagcgcaaac tgggtctcga caaccagccc attcgcaagc tgaccgatta
     1561 tcttggcgtg ctgcgcgtcg tgattttttg caccgaggat ttgcaactca tcaaaggccc
     1621 ctcacgcgcc cgcagacgtt ttgtggattt gctcctgagc caaacgcaca tcagttatct
     1681 gcccctgttg cagcgttacg cgcgggcgtt gcgttcgcgc aacgcgatat tgaaacagcg
     1741 ccaacccgac gaagccgcgc tcgacagttt ttcaaccgag ttgatcaagc tcgggaatga
     1801 aatcatccaa cgacgccgcg aacttgttcc caaactttca ccactcgcgc ggcttgcgta
     1861 tcgccgcatc tcgaatgacg ccgaagattt gcgcctggaa tatcaaccca gcgtgaaaaa
     1921 agattttgcc gtcgaactcg cgcaatcgag ccggcgcgaa cggacttacc gctcgacact
     1981 catcgggccg catcgcgatg aattcacgat gcagttgaac gaccgcgccg ccgcgcaatt
     2041 cggcagcgag gggcagaaac gcacactcgc catcgccttg aaaatggcgc aggccgaata
     2101 cctgaccggc attcacggct cgccgccgat tctgctgatt gacgacgtga tgggcgaact
     2161 cgatgtgaag cgccgcagcg gattgctccc gttgctggag cgcgcgcacc acgccaaagg
     2221 ccaggttttc atgacttgca ccgaggaaaa ctggccccgc gaacttggac gcgatctgca
     2281 acgctgggaa atcaagtcgg gcacgttgaa gaagatttga tacattttgt cgtgaactca
     2341 gcactcgata tgtaagctgt tcgagcttgt tttccaatct atgaaaaaat cgactggcaa
     2401 gagcaaaagg ccgaaaatga cacgcaagct ggctgaaacc cgatctgtag gcgcgaccaa
     2461 aggccggagc tttgacggaa cggctttcct cgtatcactt aaatccatgc atccaaggga
     2521 ttcccgactt tgattgactt tgaccgaatt caagcattca tttaggcatg actgcggcag
     2581 aaattttgca ggaattcaaa gccctcccaa tatctgaacg cgcccgcgtg gtcgaacaag
     2641 cgttgcggca attgacggcc gaagaacgca agccagtcga gcggctgatt cgccgccttc
     2701 aacatcccga agtgcccgaa tctttttggg agggggttga ggaccatgaa gatgggcgct
     2761 cggtggaaat ggatattgca ctcaaggata cgccaccggg tcgtcaatga gatttcgagc
     2821 ttcagaaacc ttctggaagc atttttacga tctcggccca acgcagaagg aatctgtccg
     2881 ccgggcgtgg aaaattttcc gagaaaaccc gtttgatcaa agattgcgaa ctcacaaaat
     2941 tcatcgtttg agcgctgcac ttggaaaaac tgtttacgcg gtcgagatcg aagctgattt
     3001 gagggcggtc ttttatttgg agggcgacgt tgcccatacg gtggacatcg gctctcatgg
     3061 tatttatcgc tgacacggcg gcatttcggt ttgaacggta acgtttgtct tgttaccttg
     3121 cttcggatgc gaattttatc tggcatacaa ccgagcggcg cgctgcatct cggcaattat
     3181 ttcgggatga tgcgcccggc aatcgaattg caggacaagg gcgagaccta ttatttcatc
     3241 gccgattatc attcgatgac gtcgctgttc gacgcgaatg aacggcgtaa aaacaccttg
     3301 gacgtggcgc tggattttct cgcgtgcgga ctcgacccga aaaaatccgt tttcttcaag
     3361 caatccgacg tgccggaagt cacggaactt tcgtggctgc tctcgacggt gacgccgatg
     3421 ggcatgctcg aacgggccac gagctataaa gacaaaattg ccagaggcat cgcgcccctg
     3481 cacggcctgt ttgcctatcc ggtgctgatg gcggcggaca ttttgattta cgattccaac
     3541 gtcgtgccgg tcggccgcga ccagaaacag cacctcgaaa tgacgcgcga catggcggca
     3601 aagttcaatg aaacttacgg ccagaccttc gtcatccccg acgcgcaaat ccgcgaggaa
     3661 accgcgctcg tccccggcat tgacggccag aaaatgagca agagctacaa caacaccatc
     3721 gagattttcg gcgaagaaaa ggccacgcgc aaaaaaatca tgggcctggt gatggacagc
     3781 cgctcgcctt ccgaacccaa gccggatgcc gataaaaacc tcgccatcca actgctcaag
     3841 ctggtcgcgc cagcagatgt cgcaaaagat tttgaggacc gtttgcgcgc aggcggattg
     3901 ggctatggcg atttgaagaa ggcgctgttc gaacattatt ggaattattt cgccgccgcc
     3961 cgcgccaaac gcgccgagtt ggtcgcaaat ttggaccacg tgaacagcgt gctcgcggat
     4021 ggcgccgcgc aggcccgtaa cttggcccaa aaagttttga aacgcgctaa agtggcgagc
     4081 gggttggatt gaagctccac gaccagcgtc taaaagctcc aaatttacag catcaatttc
     4141 gctggaggtt ggtgcttgat gtttggatgt tggagctttt caaaggtggt gcttcaaaaa
     4201 ccattcgtac agttccggat tgttgtaagt ctccgtccac gaattatggg tcgcctcggg
     4261 ataaatggtg agctttgcgt ccgcgttgcc ggctctcttg aaaatttcaa tcatccgctt
     4321 cgattcttcc ggtgacacga cgttgtcctt tccaccgtga aaagcccaca caggcagttt
     4381 cttcaacgcg ggcgcttttg atttgtcggc cagcgagagc acgaccccga tgttgccttc
     4441 gccgccgcaa atgggcgcaa ccgccgcgaa acgctcggga taagtcgtag ccaaactcca
     4501 tgtgccgtaa ccgcccatgc tcaagccggt caaataaatc cggttcgtgt ccacggcata
     4561 cttcgctgtc accgcgtcaa gaatgccgag cacgatttcg tccgaccatt tgcggccttc
     4621 gggacattgc ggcgtgatga ggatgaacgg aaaatcggga tgtttttcga t
//
LOCUS       DASZMM010000052         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4410404, whole genome shotgun sequence.
ACCESSION   DASZMM010000052 DASZMM010000000
VERSION     DASZMM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4410404"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VG754_01300"
     CDS             <1..709
                     /locus_tag="VG754_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWF17870.1"
                     /translation="THEGISALKDIMSERIQTSSKKGSVSPKTIGQKKYVDAIRHHDV
                     TFGVGPAGTGKTYLAMAMAVSALKEEKISRIVLTRPAVEAGEALGFLPGDLYEKITPY
                     LRPLHDALQDMLPAEEIQKYMERNVIEIAPLAYMRGRTLNNSFIVLDEAQNATAEQMF
                     MFLTRLGLNSKAVITGDETQVDLPPHKHSGLVEAHRALRGVEGIAIVEFTRRDVVRHP
                     LVQRIIAAYEEHRGKAG"
     gene            820..1287
                     /gene="ybeY"
                     /locus_tag="VG754_01305"
     CDS             820..1287
                     /gene="ybeY"
                     /locus_tag="VG754_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWF17871.1"
                     /translation="MNIQNIQRTKTINPGFLREIICALLNDLPGCEDYDLTVHLVGEK
                     KMSELNETILRHAGSTDVITFDYSVAGPAPMFAGEIFVCMDEALTQARRFRTTWQSEL
                     VRYIVHGVLHLQGYDDLQPIARRRMKRKENRLVGELSREFDLRKLHRKTRVSA"
     gene            1284..1967
                     /locus_tag="VG754_01310"
     CDS             1284..1967
                     /locus_tag="VG754_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HWF17872.1"
                     /translation="MNKDFFIKWRANFFAGLAVVLPGVVTLAVIFWLFFTASKITDTL
                     LIFIPQEITHETSGGVAGAGPMHWYWSLVALAVAVVLTSVVGLLARNYLGQKMIEWVD
                     NSLLRVPFLNKIYGATKQVNDAFTGNKSSFKTVVMIEFPRAGMYSIGFLTSEAESEIQ
                     CHTPEKVVCVFIPTTPNPTSGFMVLVPESQVTKLDMSVADGLKFIISLGAISPDFLPP
                     HRQKITASN"
     gene            1975..2601
                     /gene="recO"
                     /locus_tag="VG754_01315"
     CDS             1975..2601
                     /gene="recO"
                     /locus_tag="VG754_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWF17873.1"
                     /translation="MTENSIGLILRTRPLTDTSLIVHWLTPEFGRLATVAKGARRPKS
                     PFAGKLDLFYLADFSFQPSRRSELHQLREVSLRETHAAIRKELEYLQQASYCCALIEQ
                     TTEMETPLPEIFSLMTGFLTNLPRYAPQPQTLFAFEIKLLSELGLQPDFEKDKIGPDL
                     KQILRALLVSDWDALARLKLSSAQVTGLRRFLHGFMIYHLGKVPMARG"
     gene            complement(2606..>3886)
                     /locus_tag="VG754_01320"
     CDS             complement(2606..>3886)
                     /locus_tag="VG754_01320"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWF17874.1"
                     /translation="RIMNRFGDTPLGMVESALEFARIARANGFHNFKFSMKSSNPKVM
                     IECYRLLVARLDELGPDWNYPIHLGVTEAGEGEDGRIKSAIGIGSLLCDGLGDTIRVS
                     LTEDSPNEIAVCNDLLAQIPELTCSVSRLQPAFGEPPKGGTPSAWPFDPFAFERRHTP
                     EIELADGVKCGGEQTVRVVVTQATWDKVAPKIRPKDDVKPEGIYEDLNVFEIDPTVDF
                     KVNCETQLVTVKDGVQLPAITAFRLLAAKLKRLGLNNPILLKDCVNFTPVPLEPKIAL
                     LRASVVIGSLLADGIGDAILVRGESGGGQSLRLAYNILQAAGCRSFKTDYVACPSCGR
                     TLFNLQTVTASIKARTDHLKGVKIAIMGCIVNGPGEMADADFGYVGGAPGKINLYVGK
                     TPIKFNIPEAEAVDRLVDLIKEHNKWVEPEAVAA"
BASE COUNT          858 a         1058 c         1103 g          867 t
ORIGIN      
        1 cacgcacgag ggcatttccg cgctcaagga catcatgtcc gagcgcatcc agacttcgag
       61 caagaaaggc agcgtctcgc ccaagaccat cggccaaaaa aaatacgtcg atgccatccg
      121 gcatcatgat gtcacttttg gcgtcggccc ggcaggcacg ggcaaaactt acctggcaat
      181 ggcaatggcg gtgtcggctt taaaggaaga aaagatcagc cggattgttt tgacgcggcc
      241 cgcggtcgag gcgggtgaag cgcttggttt tttgccgggc gatctttacg aaaagatcac
      301 tccgtatctg cgtccgttgc acgacgcgtt gcaggacatg ctgccggcgg aggaaatcca
      361 gaagtacatg gaacgaaacg tgatcgagat cgcgccgctc gcctacatgc gcggccgcac
      421 gttgaacaac tcgttcatcg ttttggatga agcgcagaac gcgacggcgg aacagatgtt
      481 catgtttttg acgcggctgg gactgaattc gaaggcggtc atcaccggtg acgaaacgca
      541 ggttgatttg ccgccgcaca aacattccgg tttggtcgag gcgcatcgcg cgttgcgcgg
      601 cgttgagggc atcgccattg tcgagttcac gcgcagggac gtggtgcggc atccgctggt
      661 gcagcgcatc attgcggcat acgaggaaca ccggggaaaa gcgggatgaa caccctcccg
      721 cagaggagac gacgcaagga gtctcccggt tcaatttcca gtctcaaatt tgaaattcca
      781 aatccagccg cctcacaccg gtccctgcga gaacggatat tgaacattca aaacatccaa
      841 cggaccaaga cgatcaaccc gggttttctc cgtgagatca tttgcgcgtt gctaaacgat
      901 ttgccgggct gtgaagatta tgacctgaca gtccatttgg tcggcgagaa aaaaatgagc
      961 gaattgaacg aaacgattct tcggcacgcc ggctcgaccg atgtcatcac gtttgattac
     1021 tccgtcgcgg gtcccgcgcc gatgtttgcg ggagaaattt ttgtttgcat ggacgaggcg
     1081 ttgacgcagg cgcggcggtt tcgcacgacc tggcaatccg agttggtccg ttacattgtt
     1141 catggggtgt tgcatctaca aggatatgat gacctgcagc ccattgcgcg tcgccggatg
     1201 aagcgcaaag aaaaccgcct ggtgggtgaa ttatcgcgtg agtttgactt gaggaaactg
     1261 catcgcaaaa ctagagtaag cgcgtgaata aagacttttt tatcaaatgg cgggccaact
     1321 tttttgctgg ccttgccgtt gtgctgcccg gcgtcgtcac gctggcggtg attttctggc
     1381 tgttcttcac ggcctcgaaa atcacggaca cgttgctgat ctttattccg caggaaatca
     1441 cccacgaaac cagcggcggc gttgcgggcg cgggaccgat gcattggtat tggagccttg
     1501 tcgcgttggc agtcgcggtg gttcttacgt ccgtggtggg cctgcttgct cgcaattatc
     1561 tcgggcaaaa aatgatcgaa tgggtggaca attcattgct gcgcgtgccg tttttaaaca
     1621 agatttacgg cgcgacgaag caggtcaacg atgctttcac cggcaacaaa agctcgttca
     1681 aaacggtggt gatgattgaa tttccgcgcg cgggaatgta ttcgatcggt tttcttacga
     1741 gcgaggcgga gtcagaaatc caatgtcata cgccggaaaa agtggtctgt gttttcattc
     1801 ccaccacacc caatcctacc tcgggtttca tggtgctcgt gcctgaatcg caggtgacca
     1861 aattggacat gtcggtggcc gacggcctga aattcatcat cagccttggc gcaatcagcc
     1921 cggatttttt gccgccgcac cgccaaaaaa tcaccgcgag caattgaaac tggcatgact
     1981 gaaaacagca tcggcctgat tttgcggaca cgcccgctga cggacaccag cttgatcgtg
     2041 cattggctga cgccggagtt tggccgattg gcaaccgttg ccaaaggcgc gcgacggccg
     2101 aagtctccgt ttgcgggcaa gctcgattta ttttatctcg ctgattttag ttttcagccc
     2161 agccgccgtt ccgaattgca tcaacttcgc gaagtcagcc tgcgcgagac gcacgccgcc
     2221 atccgcaagg aactggaata tcttcagcag gcatcgtatt gctgcgcgct catcgagcag
     2281 acaacggaga tggaaacgcc gctgccggaa attttttctt tgatgacggg ttttctcaca
     2341 aatctgccgc gatatgcacc tcaaccgcaa accttgttcg cctttgagat caaactgctc
     2401 agcgaactcg gcctgcagcc ggattttgag aaggataaaa tcggacccga cctgaagcaa
     2461 atcttgcgcg cgctgcttgt gagcgattgg gacgcgctgg cgcgattgaa attaagttca
     2521 gcgcaagtca ccggcctgcg ccggtttttg catgggttta tgatctatca cttgggcaag
     2581 gtgccgatgg cgcgggggta ggtgatcatg cagcaaccgc ctccggctcg acccatttgt
     2641 tatgttcctt gatgaggtcc accaaacggt ccacggcttc ggcttcgggg atgttgaatt
     2701 tgatgggagt tttgccgacg taaagattga tcttgccggg tgcgccgccg acatagccga
     2761 aatcagcgtc ggccatttcg ccggggccgt tcacgatgca acccatgatg gcgatcttca
     2821 cgcccttgag atggtccgtc ctcgccttga tgctcgcggt gacggtctgg agattgaaca
     2881 atgtgcgtcc gcaactcggg cacgccacgt aatcggtctt gaacgaacgg catcccgccg
     2941 cctgcaaaat attatacgca agccgcaacg attgtccgcc gccggattcg ccgcgaacca
     3001 gaatcgcatc gccgatgccg tccgccaaca gcgagccgat cacaacggag gcgcgcaaca
     3061 aagcgatttt tggttccaaa ggcaccggag tgaaattcac acagtccttc agcaaaatag
     3121 gattgttcaa tcccagccgc ttgagcttgg cggccagcag gcgaaaagcg gtgatggccg
     3181 gcaattgcac gccgtctttc accgtgacca attgcgtctc gcaattcacc ttgaaatcca
     3241 cggtgggatc gatttcgaag acgttcaagt cttcgtaaat gccctcgggt tttacgtcgt
     3301 ccttgggccg gattttcggc gcgaccttgt cccatgtcgc ctgcgtcaca acgacgcgca
     3361 cggtttgttc gccgccgcat ttgacgccgt cggcaagttc aatttccggt gtatggcggc
     3421 gttcgaaggc gaacggatca aacggccacg cgcttggtgt cccgccttta ggtggttccc
     3481 caaaagccgg ctgaagccgg gacaccgaac aggtgagttc tggaatctgc gcgagcaaat
     3541 cattacaaac cgcgatttca ttgggcgaat cttcggtcag cgaaacgcgg atggtatcgc
     3601 ccaacccgtc gcagagcagc gacccgatgc cgatggcgct cttgatgcgt ccgtcttcgc
     3661 cttcgccggc ttcagtgaca ccgaggtgga tcggataatt ccaatccggc ccgagttcgt
     3721 cgagtcgcgc gacaagcagg cgataacatt caatcatcac cttgggattg gacgacttca
     3781 tcgagaactt gaaattatga aaaccgttcg ctcgcgcgat gcgggcaaat tccagcgcgc
     3841 tttccaccat gccgagcggc gtgtcgccga aacggttcat gatgcg
//
LOCUS       DASZMM010000053         4839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4411277, whole genome shotgun sequence.
ACCESSION   DASZMM010000053 DASZMM010000000
VERSION     DASZMM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4411277"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VG754_01325"
     CDS             complement(<1..752)
                     /locus_tag="VG754_01325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWF17875.1"
                     /translation="MNMFKDDVAMLESTELPKRTRHRRQHAVRRTDKKRKALMKAAAI
                     DRFGPPSVLKPHLLPIPEAGRGEVLIALHAAGIGVWDAEMRGGWWPEKRPKFPLVLGT
                     DGAGTIEAVGAGVRRFRVGDQVWAFEFINRKGGFYAEHVVVNAEHVGCVPRRLDLVHA
                     GAAAVTGLTALQGIDEHLRVRKGETVLVFGASGAVGTLAVQFAKRKGARVIGTASDQH
                     AKMLVRRLGADKVIDARSKRLIEELRALAPKG"
     gene            1222..1788
                     /locus_tag="VG754_01330"
     CDS             1222..1788
                     /locus_tag="VG754_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HWF17876.1"
                     /translation="MNAYDEVKRHTGWFIALGVALIVLGSIAIITVVLTTLISVMIFG
                     WLLLIGGIFQAVHAFWVRPWSGLMLQLFIGVLNIVVGLLMVLNPGASALALTLLMAAF
                     FVVGGVFRLVSAADAHFPGRGWALFSGVINILLGVLIWLQWPASAFWVIGMFIGIDLI
                     FTGWWFITLATLAHRGTGTEPTATSPAT"
     gene            complement(1867..3315)
                     /locus_tag="VG754_01335"
     CDS             complement(1867..3315)
                     /locus_tag="VG754_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF17877.1"
                     /translation="MKIPREGRKKTGLESPVNPQLGKAALRSAGIPACGLWRLSSSQS
                     VYQVALVCNRLLQGLRFFPLVYARRVQKNISRAEYCELASLFGLQFMALGIWLVPLSA
                     VLDAHGLHAIKPYAFATSATATFVSPLIFGAMADHHASPVKVLRWLSIASALAMLLVG
                     WSVHAGWPAAIVLGCIQLYALCAAPTPSISTTIVFSRLGRSRQQFGPIRAGATLGWMV
                     GCWLVSAYRADSSVKSQGIGAIIWLTVSAFTFLLPSVDPPASAQRLTWKQRLGWDALV
                     LFKNPDHRVVFIATVLFSIPMAAFYAFTPPHLQQLGFQRVAAWMTLGQVMEITGLLAL
                     GGLLMRWRLKWIFLIALGAGVPRFFLCATDSKAGLLAGILLHGLCYALFFVTAQIYLD
                     ERVESAWRGRAQALFSLMNNGVGNLAGYLGTGWWFNACAEPTGMRWPLFWGGLGVVTV
                     GVAIYFLCAYHGIGPGARAAEIKSFAAEETTK"
     gene            3367..3885
                     /locus_tag="VG754_01340"
     CDS             3367..3885
                     /locus_tag="VG754_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWF17878.1"
                     /translation="MDYIKLPIGSEMPTIINAVVEVPLGQTNKYEYDKDLQVFRLDRP
                     LYTSVYYPCEYGFIPSTLSEDGDTLDILILADRPSFPGCFLEVRPIGMLEVVDQGVPD
                     RKILSAAAHNPSYREFKNHTDVFPHLLAEIEHFFVVYKQLENKHTEVTGWRDAAQARK
                     TILQCHERFKKS"
     gene            complement(4328..4546)
                     /locus_tag="VG754_01345"
     CDS             complement(4328..4546)
                     /locus_tag="VG754_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17879.1"
                     /translation="MTNQNQLELELAAAANRCPRVLKREQRANRANWWFNQMRQVVDQ
                     AFEWEPTPRFRPEQILLNEAQQERSLHG"
BASE COUNT         1090 a         1423 c         1286 g         1040 t
ORIGIN      
        1 attcctttgg gcgcaagcgc gcgcaattct tcaatcaaac gtttgctgcg cgcgtcaatg
       61 accttgtccg cgcccaatcg ccgcaccagc attttggcgt gctgatcgga tgcagtgccg
      121 atgacccgcg cgcctttgcg ctttgcgaac tgcaccgcaa gcgtcccgac agcgccggaa
      181 gctccaaaaa ccaatacggt ttcgcccttg cgcacgcgca gatgttcgtc aattccctgc
      241 aaggcggtca atcccgtcac agccgccgct cccgcatgca ccaaatccaa acggcgcggg
      301 acgcatccga catgctccgc gttcaccacg acatgctcgg cgtaaaaccc gcctttgcga
      361 ttgataaatt caaaggccca aacttgatcg ccaacgcgaa accgccgcac gcccgcgccc
      421 actgcttcga tcgtgcctgc gccatcggtt ccaagaacga ggggaaattt cgggcgcttc
      481 tccggccacc aaccaccgcg catctcggcg tcccaaacac caatgccagc ggcgtggagg
      541 gcgatgagca cttcgccacg gcctgcttcg gggattggaa gcaggtgagg cttcaggacc
      601 gatggtgggc cgaaacggtc aatggctgcg gccttcataa gcgctttcct ttttttgtcc
      661 gtgcgccgga ccgcgtgttg ccgccgatgc cgtgttcgtt tgggaagctc cgtcgattcg
      721 agcatcgcaa cgtcatcctt gaacatgttc ataactgaaa ttcctcctcg tcaattcatg
      781 tgctgccgcg gcaagccgct cgtgtgttat tcgtgcaaaa aagtttaccg ttcaaagcca
      841 ccgtaatctt gcgcttccac actgtccagc aacgggatga atttatttta aatggcaaca
      901 gcctcattgg gcgcctgcgg gcgcattcgc aaatgcctcc gaattttggc cagacattgc
      961 ccaccgcgtt caccgggcga aacggagcgc ggctcgatga gccgcagcaa cgcgccacca
     1021 acaggacatg cagactaatt catacactca tatttcccac gttgctgcgg gtcgccgacc
     1081 cgcgctccgg gggcggtgcg cggatgctcc cggcgtctgc gaatgcaccg ttgtacaccc
     1141 catacaaatc gcgttctttt cccaaccggt tctttgacaa gcggaatgcg gtaaggaaag
     1201 ttttttgaaa ggagagaatg catgaatgct tacgacgaag tgaaacgaca cacgggttgg
     1261 ttcatcgcgc tcggggtagc gctgattgtt ctaggttcca tcgccatcat cacagtggtg
     1321 ctgaccacgt tgatctcggt gatgattttc ggctggctgc ttttgattgg cggaattttt
     1381 caggcagtcc atgctttttg ggtgcggccc tggagtggat tgatgctgca attgttcatt
     1441 ggtgtcctga acatcgtggt cggcttgttg atggtgctga atcccggcgc aagcgcgctg
     1501 gctttgacgc ttttgatggc ggcctttttt gtggtgggcg gcgtgttcag gctggtgagt
     1561 gcggcggacg cgcattttcc aggtcgcggc tgggcgttgt tcagcggtgt catcaatatc
     1621 ctgctgggcg ttctgatttg gctccaatgg cccgcatcgg ccttttgggt catcggcatg
     1681 ttcatcggga ttgacctgat tttcaccggc tggtggttca tcaccctggc aacccttgcc
     1741 catcgcggga ctggaacaga accgaccgcg accagtccgg caacgtgaag tgaaatggct
     1801 ttttggagtg cgggaatgtg ttcgcgctca aggcgcgggc atgttccgca ttccataaac
     1861 gactttttat ttcgtcgttt cctccgccgc gaaggatttg atttccgccg cgcgagcgcc
     1921 tggcccaatg ccatgataag cgcacaggaa ataaatggcc acaccgacag tcacgacgcc
     1981 cagtcctccc caaaacaacg gccaacgcat cccggtcggt tcggcgcatg cgttgaacca
     2041 ccagcccgtg cccaaataac cggccaggtt gccaacgccg ttgttcatca aagaaaacaa
     2101 cgcctgcgcc cttccccgcc aggccgattc aacgcgttca tccagataaa tttgtgccgt
     2161 gacgaaaaaa agcgcatagc aaagtccatg cagcagaatt cccgcgagca ggccggcctt
     2221 tgaatccgtc gcgcacaaaa agaagcgcgg cacgcctgcg ccaagggcga tgagaaaaat
     2281 ccatttcaac cgccagcgca tcaaaagtcc gcccaatgca agcaagcccg tgatttccat
     2341 cacctgcccg agcgtcatcc aggccgcgac gcgttgaaag ccgagttgtt gcaggtgcgg
     2401 cggcgtgaac gcgtaaaacg ccgccatcgg gatgctgaag agcacggtgg cgatgaagac
     2461 gacgcgatgg tcgggatttt tgaacaacac cagcgcgtcc cagccaagac gttgtttcca
     2521 agtgagccgc tgagcggacg cgggagggtc aacactcggc agcaaaaaag tgaatgccga
     2581 aacggtcagc caaatgattg ccccgatgcc ttgcgacttg accgatgaat cggcccgata
     2641 cgcgctcacc agccagcagc cgaccatcca cccaagagtc gcacccgccc ggatggggcc
     2701 aaattgctgt cgcgatctgc ccaagcgcga aaaaacaatc gtggtcgaaa tgctcggtgt
     2761 cggcgctgcg cagagcgcat aaagttgtat gcagcccagc acaattgccg caggccatcc
     2821 cgcatggacg ctccaaccga cgagcaacat cgccagcgcg cttgcaatcg aaagccagcg
     2881 caagactttc accggcgaag catggtggtc cgccatcgcg ccaaatatca atggcgaaac
     2941 gaaagttgcc gttgccgaag tggcgaatgc gtacggtttg attgcatgaa ggccatgggc
     3001 atcgagcacg gcgctcaacg gcaccaacca gatgcccagc gccatgaact gtagcccgaa
     3061 caaactggcc aattcgcaat attccgcgcg gctgatattt ttctgcacgc gtctagcata
     3121 cacgagcgga aaaaaacgca agccttggag aagccggttg catacgagag caacttgata
     3181 taccgattgg ctgctggaaa gccgccataa cccgcaggca ggaatgcctg cgctacgtag
     3241 cgcagccttt ccaagctgcg ggttcacggg gctttccagc cccgttttct ttcgtccttc
     3301 gcgcggaatt ttcacccagt tgcaacagcc agacgatgag ctaacttcca aattcaggga
     3361 ggcgtcatgg attacatcaa attacccatc ggttctgaga tgccgacaat catcaatgcc
     3421 gtggtcgaag ttccgctcgg ccagaccaac aaatacgaat acgacaaaga ccttcaggtg
     3481 ttccgcctcg atcgtccgtt atacacctcc gtttattacc cttgcgaata cgggttcatt
     3541 cccagcacct tgagcgagga cggcgacacg ctcgacattt tgatccttgc ggacaggccg
     3601 agttttcccg gttgctttct cgaagtccgg cccatcggga tgctcgaagt ggtggaccaa
     3661 ggcgtgcccg accgcaaaat attgtccgcc gctgcgcata atccttccta tcgcgaattc
     3721 aaaaatcaca ccgacgtctt tccgcacctg cttgccgaga tcgaacattt cttcgtcgtt
     3781 tacaagcagc ttgaaaacaa acacaccgaa gtcaccggct ggcgcgatgc cgcccaagcg
     3841 cgcaagacca ttctgcaatg ccacgagcgg ttcaaaaaat cgtaatgcga cgcggctgcg
     3901 gaccgaaaaa ccgcccggga ccaagttggt gctttgtctc caccagtcaa gccacccctc
     3961 cgtgctcgcg tccttgccga accgcaagca acaaccctcc ctgcttttta ccaatcgccc
     4021 caaataaaac tgtccggttt tgaaaactac tcgacaagag gattagagag aatggacaga
     4081 atctttcaga aaagatttta caatcctata ttctgattat tctgttcatt ctgtcaaaaa
     4141 aactgcccgg tcactgcaag gtgcggggac ttgcaatgcc gggcgcgtgc cggaaggcga
     4201 acacctcctt ggaggttgag gaaccgtcgc catgtccggg aggatgttgt tgggtaaatc
     4261 gttaacaaag ttacatggtt acatggaacg gtgatcgcag attctaacga tgtaacaatg
     4321 gaactattta accgtgtaac gatctttcct gctgcgcctc gttcagcagg atttgctcgg
     4381 ggcgaaagcg cggcgtgggt tcccattcga acgcctggtc caccacttgc cgcatctggt
     4441 tgaaccacca gttggcacgg ttcgcccgct gttcgcgttt caagacccgc gggcaacgat
     4501 tcgctgccgc cgccagttcc agttccagtt gattttgatt cgtcatattt ggccttttgt
     4561 tatttaactg gaaccaacct agcagcggag gtaggacaca ggatgtccta gtcgaaaaat
     4621 aatttgtgag aaattgattt taatttcgaa caatcaacat cgaacgccga acatcgaatt
     4681 tacgattccc tatcgggcac atcacgcaca gccccggagc gcggctcgat gagccgcagc
     4741 aatgtgggaa ggataggcgc gcatgaatta gtctgacatg tcctgtttgt cgcgcgttgc
     4801 tgcggcccat cgagccgcgc accgcttcgc tcggtcaac
//
LOCUS       DASZMM010000054         4414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4411577, whole genome shotgun sequence.
ACCESSION   DASZMM010000054 DASZMM010000000
VERSION     DASZMM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4411577"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VG754_01350"
     CDS             <1..258
                     /locus_tag="VG754_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWF17880.1"
                     /translation="GKNYLVENAHVTVTFVEDKAVSIELPSSVVLTVTDAPEGIRGDS
                     ANNVQKSVTMETGITVQAPLFIKTGEKLKIDTRTGKYMERA"
     gene            complement(342..1571)
                     /locus_tag="VG754_01355"
     CDS             complement(342..1571)
                     /locus_tag="VG754_01355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF17881.1"
                     /translation="MIYLDVTDSCKSPLNTGIQRVVRGLFRALCYANLPVTPMLWEPG
                     LGSYCALSARERGFLEAPFAARNRKALAQPARAANPIPVWSKFARQLTHRRNRLDLAA
                     RLEKNDSILVPQIFRDGRGACVASLGRQSGAQTIAFFYDAIPWRRPDVTPPSNVKGVV
                     DYMMALAKFDLIITSSREAAGDLRACWELHDNGMKQPPIHIMAWPPDETFNATDQTTA
                     PSAPRTGARRKIICVGTLEPRKNHLTLLAAAEKLWREHRLDFQLELIGRTTRECGPRV
                     LAEVERLHKARWPVHWRRHVDNESLLAAYDECAFTVFPSLVEGYGLPIVESLMRGRPC
                     VCGGNGALGELAAGGGCLIVDQTDVTALAEGMKRLLADADIYERLSAEARARRFESWP
                     DFVEKLTEILRFQRTEK"
     gene            complement(1681..2304)
                     /locus_tag="VG754_01360"
     CDS             complement(1681..2304)
                     /locus_tag="VG754_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWF17882.1"
                     /translation="MSTIWITVCAVATAQENATLTWNASTDSHTSGYYVYYGTDSHQY
                     LYKVNAGAKTSFSVTNLPPGMTFYFAVSSYSSQGVEGVPSSEVSLSPLYFLAGSGAVS
                     STVEYLQFPDGVVFGYYDSTSYPWVYHFDMGWEYFMDAGDGQGGVYLYDCLSNTFWFT
                     APSLFPYLYDFTLNSWLYYYPNSQVPGTYTSNPRYFYDFGASQIITK"
     gene            complement(2586..3656)
                     /locus_tag="VG754_01365"
     CDS             complement(2586..3656)
                     /locus_tag="VG754_01365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF17883.1"
                     /translation="MDKANTTTAPSDSTGEKRLLLSSLADFWSLIGPLLLAAAPKTMC
                     EIGIGQGEFTAVLLEFCRRNGCHYFGIDPTADAAVIQRLESSQAQFFKNSSLAVLPEL
                     PARDVYFVDGDHNYYTVLNELRAILNGTNRWPLIFLHDVGWPWGRRDQYCSPETIPQE
                     FRHPYSTSLGALPGRAKLGPGGFSGETSNYKYGAAEEEGGHRNGVLTAIEDFLREQPH
                     AEWKFLQVPAVFGLGILCAPKKCAPEVALEFGRLENSVAQLSGFLDLLERNRLDLFWI
                     YLESVVKYRVLEGEYEKLKSAYNDLNNHATSLQQSYESLHQSYDKLAVYCDSLLSSYR
                     SLERHAHAVQKAHDALLHKNHD"
     gene            complement(3673..>4414)
                     /locus_tag="VG754_01370"
     CDS             complement(3673..>4414)
                     /locus_tag="VG754_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17884.1"
                     /translation="SHAQRERETLAKYFKDVGLLEDGAGIVDVGWHASSSRSLQMLLA
                     QSGVHRRIPAFYFGTWIYAQWILEAGCQFESLFMHLSQPQHRSWIVIESVELIESFFS
                     APHPTIIGIEKKDERWQALYGQPEMDKETEEALQIVTNAGFEFIRDALAVWPRSQEIP
                     PPLGYLETTLERLLRHPTREEAIAFSKFSWRNTFGGTGPIRHLANLPSCWSRVADAEA
                     LQEAYDHCYWKKGFLAQISPKAKEYIEL"
BASE COUNT         1073 a         1214 c         1168 g          959 t
ORIGIN      
        1 ggaaaaaatt atctcgtgga aaatgcccac gtcaccgtga ccttcgtcga ggacaaggct
       61 gtttccattg agttgccttc cagcgtggtg ttgaccgtga cggatgcgcc cgaagggatt
      121 cgcggcgatt ccgccaacaa cgtccagaag tcggtgacca tggaaaccgg catcaccgtg
      181 caagcgccgc tgttcatcaa gaccggcgag aagctcaaga ttgacacgcg aacgggcaaa
      241 tacatggaac gcgcctgacg cttcgcgtca tctcaaatct gaaatttcaa atttcaaatt
      301 tgaaatttga aatttgaaat taaaagaccg cgattgagaa atcatttctc agttctttga
      361 aatcttaaaa tctccgttaa tttctcgacg aaatccggcc aggattcgaa tcgccgcgcg
      421 cgcgcttcag cagacaaacg ctcgtaaata tctgcatctg ccagcaagcg tttcatgcct
      481 tcggcaagcg ccgttacatc cgtttgatcc acgatgaggc agccgccgcc ggcagcgagt
      541 tcgcccagcg cgccgtttcc accacaaaca caaggccgtc cgcgcatgag gctttcgaca
      601 atcggcaaac cgtatccttc gaccaatgaa ggaaacacgg tgaacgcaca ttcgtcgtaa
      661 gcggccagca atgattcatt gtccacatgc cggcgccaat ggaccggcca gcgcgccttg
      721 tgcaaacgct ccacttccgc cagcacgcgc gggccgcatt cccgcgtcgt ccggccgatg
      781 agttcaagtt ggaagtccaa gcgatgctca cgccacaact tttccgcagc agcaagcagg
      841 gtcagatgat tcttgcgcgg ttcaagcgta ccgacgcaaa ttattttgcg acgagcgcct
      901 gttcgcggcg cagatggagc ggttgtttga tccgttgcat tgaatgtttc atccggcggc
      961 caggccatga tgtggatcgg cggttgtttc atcccgttgt catgcaattc ccagcatgcg
     1021 cgcaaatctc ccgctgcttc tcgcgaggaa gtgatgatca aatcaaactt cgcgagtgcc
     1081 atcatgtaat ccacaacgcc tttgacattc gaaggcggag tcacgtccgg cctgcgccac
     1141 gggatggcat cgtagaagaa ggcaattgtc tgcgcgccgg attgccggcc gagagacgcg
     1201 acgcacgcgc ctcgtccatc gcgaaaaatc tgcggcacga gaatcgaatc gtttttttcc
     1261 aaacgggcag cgagatccaa acgattgcgg cgatgggtga gctggcgcgc gaacttgctc
     1321 caaacaggaa ttggattcgc agcgcgcgcc ggttgcgcga gcgccttgcg gttgcgcgcc
     1381 gcaaatggcg cttccagaaa accgcgttcg cgggcggaca atgcgcagta acttcccaat
     1441 cccggctccc acaacatcgg tgtcaccgga aggttcgcgt agcacaaagc gcggaaaagc
     1501 ccgcgcacaa cacgctggat gccggtgttc aacggcgact tgcatgaatc ggtgacgtcg
     1561 agataaatca tgcgcgcgga cagatgaagg cccgcgtttg tgtcggcgtg cgaaaagggc
     1621 ggcgcatgat gctattaacc gcccttgcgc cgaagcgctc aagaagaaat taagcgctct
     1681 ttatttggtg atgatctggc tggcgccgaa atcgtagaaa taccgcgggt tggaagtata
     1741 agtgccaggc acctgcgaat tggggtagta atataaccac gagttcaggg tgaagtcgta
     1801 aagataagga aacaacgacg gcgcggtgaa ccagaacgtg ttgctcaaac aatcgtagag
     1861 gtacacgccg ccttgtccat ctccggcatc catgaaatat tcccaaccca tgtcgaaatg
     1921 atatacccaa ggatagcttg tcgaatcgta atagccaaac acgacgccat cgggaaattg
     1981 caaatactcc acggtgctgg aaacagcgcc gctgccggca aggaagtaca atggcgacaa
     2041 cgaaacctcg ctcgaaggaa cgccttcaac accttgagac gagtaggaac tcaccgcaaa
     2101 gtaaaaggtc attccaggcg ggagatttgt taccgaaaag ctggtcttgg cccccgcgtt
     2161 caccttgtac aaatactgat gactgtctgt gccatagtaa acgtaatagc ccgaggtatg
     2221 gctgtcggtt gatgcgttcc atgtgagggt cgcgttttcc tgcgcagtcg ccaccgcgca
     2281 gacagtaatc cagattgtgc tcacgcacag gcgaatcatt cccgataaca aattgggctt
     2341 ggattgagcc cgtccaaaaa cgtatttcat cctgttgttg gtaaaagcag aaacgttgcc
     2401 agcagttgta aacccgtctc aaatcaggcc actgcacaga cttatgagtc agctcgcgta
     2461 aaattgcgcg aattcagtgt ccaaagggca aaccccttgg cgcaaaaccg tacatgtgct
     2521 gtaccggatt cagacaaaag attttattga gatgccctta acgtccaatt actggcggat
     2581 ttggcctaat cgtggttttt gtgcaagagc gcatcgtgag ctttttggac ggcatgcgcg
     2641 tgccgttcaa gggaacgata gctggataac aacgagtcac agtagacggc cagtttgtca
     2701 tagctctggt gcaatgattc ataactttgt tgtaatgagg ttgcatggtt gttcaagtcg
     2761 ttgtaagcgg acttgagctt ttcgtattca ccttcgagaa cgcgatactt taccacactt
     2821 tccaaataaa tccagaaaag atccagccgg ttgcgttcaa gcaaatccag aaacccgctg
     2881 agctgcgcaa cactattttc caagcgccca aactcaagcg caacttccgg cgcgcatttt
     2941 ttcggcgcgc aaagaatgcc cagcccgaac accgccggca cttgcaaaaa tttccattcg
     3001 gcatggggct gctcgcgcag gaaatcttcg atggccgtaa gcacgccgtt gcggtgccct
     3061 ccttcttctt cggctgcccc atatttgtag ttgctcgtct ctccggaaaa accgccgggg
     3121 ccaagtttcg cgcggccggg caatgcgccg aggcttgttg aatacggatg acggaattcc
     3181 tgcggaatcg tttccggcga acaatactgg tcgcgtcgtc cccatggcca gcccacgtca
     3241 tgcaggaaaa tcaacggcca acggttcgtt ccattcagaa tcgccctcaa ctcgttgagc
     3301 acggtgtagt agttgtgatc gccgtcaacg aaatacacgt cccgtgcggg cagttcgggg
     3361 agcactgcca aggatgagtt tttgaaaaat tgcgcctgag aactttccaa ccgttggata
     3421 accgcagcgt ccgccgttgg atcaatgccg aaataatggc aaccattccg ccggcaaaat
     3481 tccagcaaga ccgcggtgaa ttctccctgg ccaatgccga tctcgcacat tgttttcggt
     3541 gcggcggcca aaagcagcgg cccaatcaag ctccaaaaat ccgcaagcga agaaagtagc
     3601 agccgttttt cgccggttga atcggacggc gcagtggttg tgttggcctt gtccatgcaa
     3661 acgaatcaca cattaaagct cgatgtactc cttcgccttc gggctgattt gcgcgaggaa
     3721 gccttttttc caataacaat ggtcgtaggc ttcctggagg gcttcagcgt cggcaacacg
     3781 gctccaacac gaaggaaggt tggccagatg ccggatcggc cctgttcctc caaaggtgtt
     3841 gcgccaggaa aatttgctga aagcaatggc ctcctcccgc gtgggatgcc gcagcagacg
     3901 ttccaacgtg gtttcaagat aacccagcgg cggcgggatt tcctgcgaac gcggccagac
     3961 ggcgagagcg tcgcggatga attcaaatcc ggcgttcgtg acgatttgca gagcctcctc
     4021 cgtttccttg tccatttccg gctggccata caaggcctgc catctttcgt cttttttctc
     4081 gatgccgatg atggtcgggt gaggcgcgga gaaaaatgat tcaatcagtt caacgctttc
     4141 aatcacaatc catgaccggt gttgcggctg gctcagatgc atgaaaagcg attcgaactg
     4201 gcatccggcc tcaaggatcc attgggcata tatccatgtg ccgaaataaa acgccggtat
     4261 gcgtcgatga acaccggatt gcgccagcag catttgcaag gaccttgacg acgaagcatg
     4321 ccagccgaca tccacaatgc ccgcgccatc ttcaagcagc ccgacatcct tgaaatattt
     4381 cgccagagtt tcccgttccc gttgcgcgtg cgac
//
LOCUS       DASZMM010000055         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5235043, whole genome shotgun sequence.
ACCESSION   DASZMM010000055 DASZMM010000000
VERSION     DASZMM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5235043"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..416)
                     /locus_tag="VG754_01375"
     CDS             complement(57..416)
                     /locus_tag="VG754_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF17885.1"
                     /translation="MKKAELKSFGKPEEVREFPKGRLEMIKVGGAMVGRAIFEPGWRW
                     ATSVQPIAKTHSCQAPHFQYHVSGVLRIKMDDGTEFDCKPGDVSLLPSGHDAWVVGNE
                     PAVVVDFQGMIDYAKGA"
     gene            complement(713..1384)
                     /locus_tag="VG754_01380"
     CDS             complement(713..1384)
                     /locus_tag="VG754_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17886.1"
                     /translation="MKTLKLTWTIARGIASVLFAVATSPFAGFTALFFALFMGVALAL
                     VLGLGFHTQLLWKLALPLLILSAVLAFRIGLPRMPAVSGPTVYDKAEYHYDGDDYPKG
                     LARRHAFVHTGMFVGWLIEHDMIVTDFLDETVGFKERRITGAKIYKAWDGTLISDMLT
                     EEGNRFAAHYFDFERGQFLDDYQEALASGLPSFYHVADTWDNFDIIKKKIDQRYETWK
                     RDKVA"
     gene            1794..2831
                     /locus_tag="VG754_01385"
     CDS             1794..2831
                     /locus_tag="VG754_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HWF17887.1"
                     /translation="MNHFRSLLGRMAFVPLLAAIFSTSSLRASPGEEMADAANNWLNA
                     LTPEQQAKATYPFENDERFDWHFIPKERKGLPFKEMTPAQQKLAHALLSTGLSPRGYA
                     KAVTIMSLEDVLKEMEQGKGPERNPERYFFTIFGKPGKDVWGWRVEGHHVSLNFVVRG
                     GEVLAATPAFFGANPAEVKQGPRKGLRVLREEETLGRAFVKSLSPEQRKVAILDIPAP
                     KEMITGNARKAHLLDPPGISADKLTAEQKEALHQLLNSYAQRHRPEVAETDLKKIEQA
                     GFDKVTFAWAGGLEPGDPHYYRIQGPSFLLEYDDTQNNANHIHTVWRDLENDFGDDVL
                     RRHYDEVPHDK"
     gene            2892..>3299
                     /locus_tag="VG754_01390"
     CDS             2892..>3299
                     /locus_tag="VG754_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWF17888.1"
                     /translation="MNHSLSRREFLATTSLMLLAMAAGAVDASTPDPIIDIHQHTNYS
                     YRTDEQLLAHQRAMGVSQTILLPAGALYHLEANATGNQACYDFALNHPGEYVFFANEV
                     AGLPGARAEISKWLKRGALGIGEQKFQVDCDSPA"
BASE COUNT          758 a          912 c          896 g          733 t
ORIGIN      
        1 caaaacatat atgattatat tgcgcacgaa ccaattttga ttcggacagc ccgctttcac
       61 gcccccttcg cgtaatcaat catgccctga aaatccacca cgacggcggg ctcgttgccg
      121 acgacccatg cgtcatggcc ggaaggcagg agggagacgt cgccgggttt gcaatcgaat
      181 tccgtgccgt catccatctt gatgcgcagc acgccggaaa cgtggtattg gaagtggggc
      241 gcctggcaac tgtgggtctt ggcaatcggc tggacggacg ttgcccagcg ccaacccggc
      301 tcaaaaatcg cgcgcccgac catcgcgccg ccaaccttga tcatttccaa tctgcccttg
      361 gggaactcgc gcacttcctc cggctttccg aaacttttta actccgcttt tttcatattt
      421 ttccttttgc ttgttagttg tttcaaagaa acgggtcagt gaggcttacg agtgcagtgt
      481 ggactcttta accggaaagc tcaattcaaa tctcgatcgt ccgaagacac ccgtagcgca
      541 gatttttaat ctgctgtatc gcggaatttc attccgcaag gtccgtgaaa tttgcggcag
      601 tctcctaatt ttcgagcggt tcgtcattcg tcctccctgc cgactgcaag tcggcgatac
      661 ggcagaatgc aattctgcgc tacgccttcg gacagatgcg cccgacgaag ccttaggcga
      721 ctttatctct tttccaagtt tcgtatcgct ggtctatttt cttcttaatg atgtcaaaat
      781 tgtcccaggt atccgcaaca tgatagaagg aaggcaggcc gctcgccaaa gcctcttgat
      841 agtcatccaa aaactggccg cgctcaaaat cgaagtagtg cgcggcaaat cggtttccct
      901 cctcggtcag catgtcactt atcaaagtgc cgtcccaagc tttgtaaatc ttagccccgg
      961 taatcctccg ttccttaaat ccgacagttt catcgaggaa atcagttaca atcatgtcat
     1021 gctcaatgag ccaacccaca aacatgccgg tatgaacaaa cgcatgcctt cgagcgagac
     1081 ccttcgggta atcgtcgcca tcataatgat actcggcctt gtcatatacc gttgggcctg
     1141 atacggccgg catccggggc aatcctatgc gaaacgcaag aactgcgctc aaaatcaaaa
     1201 gtggcaatgc cagtttccaa agcaattgcg tgtgaaatcc aagcccaagg accagcgcca
     1261 atgccacgcc cataaaaaga gcaaagaaca gcgcagtgaa acccgcgaaa gggcttgtgg
     1321 ctaccgcaaa taacacggat gcgatgcctc tggcgatcgt ccatgttagt ttaagcgtct
     1381 tcattacaac tggccagcgt gagtaacgaa acaagaaaca ataagcaggc tgttttcata
     1441 gtgtttgcgc tcccaactcg agtgaaatct gttatgccaa aattgataat caaaacggtc
     1501 caatcctgca agcgcgtcgt tgccgaaccg gccaaacgtt gtaacgcaga ttttcaatct
     1561 gctgtatcgc ggaatttcat tccgcaaggc cgggacattg cagcggggcg gaaattttca
     1621 agcgcgctgc ccgtttcatc gccctgccga ctgcaagtcg gcgatacggc agaatgcaat
     1681 tctgcgctac gccttcgggc aggatgccgg ctttacggca ggctggacgc ccgccgccac
     1741 tgcgcgagcg ttttttaatt gcctttcgcc cgttgtttgc tgaccttcaa gcgatgaatc
     1801 actttcgttc gttgttgggc aggatggcgt tcgttccatt gctcgccgca attttttcca
     1861 catcgtcttt gcgcgcgagt ccgggcgagg agatggcgga cgcggcgaac aattggttga
     1921 atgcgctcac gccggagcag caggccaagg cgacttatcc gttcgagaat gacgagcggt
     1981 tcgactggca tttcattccc aaggagcgaa agggtttgcc gttcaaggaa atgacgccgg
     2041 cgcagcagaa gctcgcgcac gcgttgctca gcaccggcct cagcccgcgc ggttacgcga
     2101 aggcggtcac gatcatgagc ctcgaggatg tgctcaagga aatggaacaa ggcaagggac
     2161 cggaacgcaa tccggagcgt tatttcttca cgattttcgg caagcccggc aaggatgtgt
     2221 ggggttggcg cgtcgagggc catcatgttt cgctgaattt tgtcgtgcgc ggcggcgaag
     2281 tgctcgcggc gacgcccgcg ttttttggcg cgaacccggc ggaagtgaag caagggccgc
     2341 gaaaagggtt gcgcgtgttg cgcgaggaag aaacgcttgg gcgcgcgttc gtgaaatcgc
     2401 tttcgccgga gcagcgaaag gttgcgattt tggacatccc cgcgccgaag gaaatgatca
     2461 cgggcaacgc gcgcaaggcg catctgctgg acccgccggg catctcggcg gacaagttga
     2521 ccgcggagca aaaggaggcg ttgcatcaat tgctcaacag ctatgcgcaa cggcatcggc
     2581 cggaagtcgc ggagacggat ttgaagaaga tcgagcaggc gggttttgac aaggtgacgt
     2641 tcgcatgggc tggcggtctc gaaccgggcg acccgcatta ttatcgcatc caagggccgt
     2701 cgttcctgct cgaatacgac gacacgcaaa acaacgcgaa tcacatccac accgtctggc
     2761 gcgacctgga aaatgatttc ggcgacgatg tccttcggcg gcattacgac gaagtgccgc
     2821 atgataaatg aggttacctg cgagaagtcg tgcgatgaaa ctttcagttt tacgctcaca
     2881 aagtttaagc gatgaaccat tctctttccc gccgcgaatt tttggcaacg acttcgttga
     2941 tgttgctcgc catggccgca ggcgccgttg atgcgtccac gcccgatccc atcatagaca
     3001 tccatcagca cacgaattac agctatcgca ccgatgaaca attactggcg catcaacgcg
     3061 ccatgggcgt ctcgcaaacg attttgctgc cggctggcgc gctttaccac ctggaggcca
     3121 acgcgacggg caaccaggct tgctacgatt tcgcgctgaa tcatcccggc gaatacgttt
     3181 ttttcgcgaa cgaagttgcc ggattgcccg gagcgcgcgc ggagatttcc aaatggttga
     3241 aacgcggagc gctcggcatc ggcgagcaaa aattccaggt ggattgcgat tcgccggcg
//
LOCUS       DASZMM010000056         5380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1664754, whole genome shotgun sequence.
ACCESSION   DASZMM010000056 DASZMM010000000
VERSION     DASZMM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664754"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VG754_01395"
     CDS             <1..302
                     /locus_tag="VG754_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17889.1"
                     /translation="LGDSIEANPVWTPTGKHIVFASDRAQNDLKQKNYDIWISDADGS
                     KQTQLTSNGSFDASPAISSDGKYLYFFSNRGAQRTGQESLQIFRLELPSEYAPAK"
     gene            complement(423..800)
                     /locus_tag="VG754_01400"
     CDS             complement(423..800)
                     /locus_tag="VG754_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17890.1"
                     /translation="MKKRWLADWPWETVVAINAGLCKEKNALHKPTSDGYQPAIKLWN
                     DSRKQELTLREAISVCRQCHKLAPFCFYNGNTFAAIGRTMVQDILRNLSPAKAHSFRS
                     VVGHYIAGTTGEDELAKALDLIG"
     gene            complement(797..985)
                     /locus_tag="VG754_01405"
     CDS             complement(797..985)
                     /locus_tag="VG754_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17891.1"
                     /translation="MATTSQAQKNLRNAMKEALRIQDRFVAEPLFAKTGHGYLKSEGI
                     ATIQKIKANLRRRAAKPK"
     gene            complement(1293..1763)
                     /gene="secG"
                     /locus_tag="VG754_01410"
     CDS             complement(1293..1763)
                     /gene="secG"
                     /locus_tag="VG754_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWF17892.1"
                     /translation="MTILIVLATILLILDCLVLGLLILVQLPKKEAGVGMAFGGGAAD
                     ALFGAGSGTALSQLTKYTATAFFALVFLLSVMNNHNRGGNASELRRQMEKMPLTTTPT
                     TPPAPAPANNPASSSSTPVIPLSISSNTVAAPTPPSKPAPTPAVPAATNATQPK"
     gene            complement(1949..2713)
                     /gene="tpiA"
                     /locus_tag="VG754_01415"
     CDS             complement(1949..2713)
                     /gene="tpiA"
                     /locus_tag="VG754_01415"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWF17893.1"
                     /translation="MKERKLIIAGNWKMNKTAAEGLALVHDLKLELANIKEADIIVCP
                     PFTALSDVSKEILNSNIRLGAQNMNEHNHGAYTGEIAAGMLKEFSVRYVILGHSERRQ
                     YQKETDALIAKKALAAHAAAIKPIVCVGETLAEREANQTEKVLDTQVRGSLAGLTKAQ
                     MEETILAYEPVWAIGTGKTATAAQAQEAHLFIRQLLGSLFDENTARRVRIQYGGSVKP
                     SNSRELMALPDVDGALVGGASLEARGFADIIKNAIN"
     gene            complement(2906..4165)
                     /locus_tag="VG754_01420"
     CDS             complement(2906..4165)
                     /locus_tag="VG754_01420"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWF17894.1"
                     /translation="MVKLTVKDLNVRGKRVLVRVDFNVPMEEKGGQMVINDATRIIET
                     LPTLELLVNQGAKVILMAHLGRPKGQREPSMSLRPVAAKLADLLHRPVAFVDDCIGEK
                     VEKTVGLLQPGDVLLLENVRYYNEEEANDPVFAAKLAKLGDIYINDAFGAAHRAHAST
                     EGVARLISKQGGQSAAGLLMERELKFLGDELENPARPFVVILGGAKVSDKIKVIDRLL
                     EKADTILIGGAMTYTFRLALGAKVGKSLVEPDKTDIAKAALEKAKARNVQFLLPTDNT
                     VATPVKTDKLNKKGKPVIELNNPRTNTDLDIPDAEEGVDIGPATRAAYAEVIHNAKTI
                     LWNGPMGIFEDPRFAEGTFAIARAVVEATQKNGAKSIIGGGDSVKALNQAKLGDRVTF
                     MSTGGGASLEFLEGKVLPGVAALADNG"
     gene            4374..5159
                     /locus_tag="VG754_01425"
     CDS             4374..5159
                     /locus_tag="VG754_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF17895.1"
                     /translation="MLRWFEHAQIFHPKREWVTAPGDLGRPFEDVSFAASDGVRLSAW
                     FFPADDGSPRSRFAMLVCHGNGGNISHRLDLCSSFLRTGVNVMLLDYRGYGHSEGKLS
                     EEGTYLDVQAAHHWLRGRGFEPQNIIVFGESLGGAIACELALREQIGGLILQSTFTNI
                     ADIGAELFPWLPVRWLHTIKYDTWKKLPRIHVPVLVTHSRDDTLIHFHHAEKNFAAAN
                     EPKMFWETSGGHNYSNISDYDRCVEGIEKFLSRIEENAVKTHS"
BASE COUNT         1162 a         1460 c         1498 g         1260 t
ORIGIN      
        1 cccttggcga ttccattgaa gcaaatcctg tctggacgcc gaccggcaag cacatcgtct
       61 tcgcgtcgga cagggcgcaa aacgacctca agcaaaagaa ttacgacatc tggatttcag
      121 acgctgatgg cagcaaacaa acgcagctca cctccaacgg ctcgtttgac gcttcaccgg
      181 ccatcagttc cgacggaaaa tatctctatt tcttttccaa ccgcggcgcg caacgcaccg
      241 gccaggaatc gctgcaaatc ttccgcctcg aactccccag cgaatacgcg ccggcgaagt
      301 gaggagtgcc ggcctgcaaa acaaaaataa tcgcggagta atgttgtagc tcatgttgcg
      361 ggttagcgat tggttacgcc aaggcggtgc tgtgcagcct ccggtacacc tcatattttg
      421 gatcatccaa taaggtcaag cgccttggcc aattcatctt cgccagtagt tccggcgatg
      481 taatgaccaa ccacactccg aaaactgtgg gctttcgcag gcgaaagatt gcgaagaatg
      541 tcctggacca tggtcctgcc aatcgcggca aaagtgttgc cgttataaaa acaaaatggg
      601 gcgagcttat ggcattggcg gcagacagaa atggcttcgc gcaaagttag ttcctgtttt
      661 cgagaatcgt tccagagttt gatggcgggt tggtaaccgt cggaagttgg cttgtgcagg
      721 gcattctttt ctttgcacaa cccggcattg atggcgacca ctgtttccca tggccagtcc
      781 gccagccatc gtttcttcat ttcggctttg ctgcgcggcg gcgaagattg gctttgatct
      841 tttgaatggt cgcaatcccc tcgcttttaa gataaccgtg gccggtcttc gcaaaaagcg
      901 gctcggcaac aaatctgtcc tggatgcgca atgcttcttt catcgcgttg cgaaggttct
      961 tttgggcctg gctcgttgtc gccataagcc aaattaaagc catttccatc aattggtcaa
     1021 cactgcatcg tgcatccaga ggaacacaaa ggtgaactac ctgcgatttg ttggccattc
     1081 gggacggact ggccgggcca ttgctcgccg cgttgaccgg acgaaacgga gcgcggctcg
     1141 atgagccgca gcaacgcgcg acgaacagga catgccagac taattcatac actcatactt
     1201 cccacattgc tgcgggtcgc ccacccgcgc tccgggacaa tgcgcggatg cacctgcgga
     1261 caacaacata ctgcggttgt tccgcgcttt gctcacttcg gttgcgtggc atttgtggct
     1321 gcgggaacag caggcgttgg agcaggtttg ctgggaggtg tcggcgctgc aacggtgttg
     1381 ctcgaaatgg aaaggggaat gacaggagtc gagctgctgg acgcgggatt gttcgcagga
     1441 gcgggcgcag gcggtgtcgt tggcgtggtc gtcaagggca ttttttccat ctggcggcgg
     1501 agttcagaag cgttgccgcc gcggttgtgg ttgttcatga cggagagcaa aaacaccagc
     1561 gcgaaaaaag ccgtggctgt gtatttggta agctggctca acgcggtgcc cgagcccgcg
     1621 ccgaacaggg cgtccgccgc gccgccgcca aaagccatgc caacgcccgc ttccttcttg
     1681 ggcagttgaa caaggatgag cagccccagc acgaggcaat cgagtatcag gaggatggtc
     1741 gccaaaacaa tcaggatggt cataatggta atttcgtaat tgagttaaat tcagttcagt
     1801 cggtttactt tcagcaggcg actggaaagt cgcctgaacc cgcaggctgg aaagcctgcg
     1861 ctacgcagcg cagacattct tgtctgcggg ttgcggggac tttccagtcc ccattccaaa
     1921 tcaaagttga aatacagcaa tagttcattc agttgatggc atttttgatg atgtcagcaa
     1981 agccgcgcgc ttcgagcgaa gctccgccca ccaacgcgcc gtccacgtcg ggcaacgcca
     2041 tcaattcgcg agagttcgat ggtttgacgc tgccgccgta ttggatgcgg acgcgccggg
     2101 cggtgttttc gtcaaacaaa ctcccaagca actggcggat gaacaggtgc gcttcctgcg
     2161 cctgcgccgc cgtggctgtt ttgccggtgc cgatggccca aaccggttcg taagccagta
     2221 tggtttcttc catctgggcc ttggtcaatc ccgccaaact gccgcgcacc tgcgtgtcca
     2281 aaactttttc ggtctggttg gcttcgcgtt cggccagtgt ttcgccgacg cagacaatcg
     2341 gcttgatcgc cgccgcatgc gccgcaagcg ctttcttcgc gatcagagca tcagtttctt
     2401 tttgatactg acgccgctcg gaatggccga gaatgacata tcggacggaa aattccttca
     2461 acatcccggc ggcgatttcg cccgtgtacg cgccgtgatt gtgctcgttc atgttttgcg
     2521 cgccgagacg gatgtttgaa tttaaaattt ccttggagac atccgagagc gcggtgaacg
     2581 gcgggcaaac gatgatgtcg gcttccttga tgttggcaag ttcgagcttg agatcgtgca
     2641 ccaaggcaag cccctcggcg gcggtcttgt tcatcttcca attgccagca atgatgagtt
     2701 tgcgttcttt catggggagg gggaaatgga gtgatggagc gctggagtgg ttgggaaatg
     2761 gagtagtgga gtattggagt gatggctgcg actcttccac ccaatactcc aacactccaa
     2821 cactccaaca ttccaacatt ccaatggttg gaaatgaaaa tttactaatg gctgagagtt
     2881 tcttacgcat gatttcttcg gcccgtcagc cgttgtccgc caatgccgcg acgccgggca
     2941 ggactttgcc ttccaaaaat tcaaggctcg cgccgccgcc ggtgctcatg aaggtgaccc
     3001 gatcgccgag cttggcctgg ttcaacgctt tcacgctgtc gccaccgcca atgatgcttt
     3061 tggcgccatt tttctgcgtc gcttccacaa cggcgcgggc gatggcaaaa gttccttccg
     3121 caaaacgcgg atcttcaaaa atccccatcg ggccgttcca caaaatcgtc ttcgcgttgt
     3181 ggatcacttc cgcataagcg gcgcgtgttg ccgggccgat gtccacgcct tcctcagcat
     3241 cgggaatgtc cagatcggtg ttggtgcgcg gattgttcag ctcgatgacg ggcttgccct
     3301 ttttgttgag cttgtcggtt ttgaccggcg tggccacagt gttgtctgtc ggaaggagaa
     3361 attgcacgtt gcgcgccttg gctttttcca gcgcggcttt ggcgatgtcc gtcttgtccg
     3421 gctcgacgag tgatttgcca actttcgcgc ccaaagccag ccggaacgtg taggtcatcg
     3481 cgccgccaat caaaatggtg tcggcttttt ccagcaaacg gtcgatcacc ttgattttgt
     3541 cggacacttt cgcgccgccc aaaatcacca cgaacggacg cgctggattc tccagttcat
     3601 cgcccaaaaa tttcaattcg cgctccatca acaggccggc tgcggactgg ccgccttgtt
     3661 tggaaatgag gcgagcgacg ccttcggtgg aggcgtgcgc gcgatgggcc gcgccaaaag
     3721 cgtcgttgat gtaaatgtcg ccgagtttgg ccagtttcgc ggcgaacacc gggtcgttgg
     3781 cttcctcttc gttgtaataa cggacgtttt cgagcagcaa aacatcgccg ggctgcaaga
     3841 gaccgacggt tttttcgact ttttccccga tgcaatcatc gacaaacgcc accggacgat
     3901 gaagcagatc ggccaatttg gcggcgacag gccgcaaaga catggaaggt tcgcgctggc
     3961 ccttgggacg gccgagatgc gccatgagaa tgacctttgc accctgattg acgagcaatt
     4021 cgagcgtggg aagggtctcg attatgcgcg tggcgtcatt gatgaccatt tgcccgccct
     4081 tttcttccat gggcacattg aagtccacgc gcacgagaac gcgtttgccg cggacattca
     4141 aatctttgac cgtcaatttt accataaaac aatcaagcca aacaggggcg agaggataac
     4201 ctgccaatag cgtgagtcaa agccaatttc agacccgatt tgaagacaat ttacgcgagg
     4261 aattggggtt gtccagcagc ggaaaggcgg tgtttggccc ggttcgaatc ttgtaactcc
     4321 atcaccatta aacctttatt ggacaactct cggcgaaaaa ccgatattcc aacatgcttc
     4381 gttggtttga gcatgctcaa atatttcatc ccaaaaggga atgggtcacg gctcccggcg
     4441 acttgggacg gccatttgag gatgtttcgt tcgccgcgtc ggatggcgtg cggttgagcg
     4501 catggttttt ccctgcagat gatggttcgc cgcgttcgcg gttcgcgatg ctcgtttgcc
     4561 acggcaacgg cggcaacatc agtcatcggc tcgacttgtg cagttcgttc ttgcggactg
     4621 gcgtgaatgt catgctgctg gattatcgcg gttacggtca cagtgagggc aaattgagcg
     4681 aggaaggcac ttatctcgat gtccaagccg cccatcattg gctgcgcggg cgcggatttg
     4741 aaccgcaaaa catcatcgtc tttggcgaat cgttgggcgg cgcgattgcg tgcgaactgg
     4801 ccctgcgcga acaaatcggc gggcttatcc tgcaaagcac tttcacaaac atcgcggaca
     4861 tcggcgcgga actttttccc tggctgccgg tccgctggct gcacaccatc aagtacgata
     4921 cttggaaaaa attgccccgc atccatgtgc cggtgctggt cacgcatagc cgcgatgaca
     4981 cgcttatcca ttttcatcac gcggagaaaa attttgccgc cgcaaatgag cccaagatgt
     5041 tctgggaaac cagcggcggc cacaactatt ccaacatcag cgattacgac cggtgtgtcg
     5101 aaggcatcga gaaattcctc agtcgcatcg aggaaaacgc ggtgaaaacg cattcgtaaa
     5161 aagcgttttt caattttttt tctgcacact tcgcggcctt cgcgcgaaaa tttccgttct
     5221 catcaatgcg tgcgcacacg ataaaagcga tgttggagat tgcttccttc aaaatcggcg
     5281 caacccatcc aaacattgga tgtggcgacg gatgtctggc caatcgtcgc ccaagttttc
     5341 aaatcggttg aagcctggat ttcataccaa tgcgatggca
//
LOCUS       DASZMM010000057         7257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3861979, whole genome shotgun sequence.
ACCESSION   DASZMM010000057 DASZMM010000000
VERSION     DASZMM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3861979"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VG754_01430"
     CDS             <1..834
                     /locus_tag="VG754_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWF17896.1"
                     /translation="GGTSYTVGANLAGQTDAQGQGWFQAGSTGPQPIISAGNLTISGL
                     ASSGGNSVSYGVPAGSPTISARFNLGSTVASGTLYYSMALQVTSLGSLTTGGSFIAGF
                     NNSAGAQTGTPTTFATRLYTRLSGTGFNVGLDLNGTSSTQYGSTTYNVGDTLFIVGSY
                     TFISGTANDTASMWLNPDSSTFGAVTAPASTLTSASSAESDIASIASFMLRDGSVVEP
                     GLIVDDVRIGNNWADVTPAVPEPATTMLGGMGLGVLALIRLVRSKLNKRQLVSRSLLL
                     S"
     gene            complement(831..1562)
                     /locus_tag="VG754_01435"
     CDS             complement(831..1562)
                     /locus_tag="VG754_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HWF17897.1"
                     /translation="MKPHPNPKRQAFTLIELLVVIAIIAILAALLLPALSKAKEKTKR
                     ISCMNNLHQLGLGCQMYANDDEKHNYTSEPNLSDDDQNFLLPYVPNLNTYICPSTQNF
                     IRPNIFNNEITDLTQTAASKKYVPGTSYEVYGWYHRYNGRSPIKKTEPNVLAYAHEMN
                     AFNLLGMVPGPSQTWLMVDADQPQPLAGGNENYPDPCDNHGAAGDNVNFCDGHAEFIT
                     QQNFIYRYELSEDSGRTQISPIYGP"
     gene            1816..2919
                     /locus_tag="VG754_01440"
     CDS             1816..2919
                     /locus_tag="VG754_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17898.1"
                     /translation="MKIKKATSFGLIACILCVAAPMLEALPFAETFNNDPAGNLGGQL
                     NAEGNPWTPMGTNGTTGTADVTVTNFNLAYPGLECPGTNSVLFGYLGYTERVAINPPV
                     GTNAFGTTGGTIYYSYVFYVTNVAQLTSSGAFVSAFTTVTGTSATQPTVGGARLYMKL
                     SSINPSTAVNVGIGKNPASPSFVTWATNEINVGDTNFIVAAYTFFNSGASNDVASMWV
                     NPNSNTFGAVFSPPPDVTNTNGTDVQVSGANQIESFYLRQGNSSIPLVLASNLRLGYY
                     WGCVTPPSDPGPISEANMSLLASGTNLTLSWGTNAPCFTLQSATSLASPFTNVVPLSA
                     FTLDATSNAYTNIITLQSNSPTSKFYRLKQVAN"
     gene            3185..4771
                     /locus_tag="VG754_01445"
     CDS             3185..4771
                     /locus_tag="VG754_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HWF17899.1"
                     /translation="MQLHGKNLIGGRISGRGTVSFQGVIAGSGEKIAPQFFEATEDEV
                     DEALRLAAEAFEKYRVQPPEKIAAFLDQIGEEIMGAGDELLRRANAETALPESRLTGE
                     RARTVNQCKMFAELVREGSWVEARIDRAIPDRQPLPKPLLQRMLISIGPVIVFGASNF
                     PLAYSVAGGDTISALAAGNPVVVKAHPAHPGTSEIVAGAVQAAAAATGMPTGVFSMLH
                     GQSTEVGIRMVRHPAAKAVGFTGSLQGGRALFDAAAARPEPIPVYAEMGSTNPVFILP
                     GALERNLDALGQGLVQSVTLGLGQFCTKPGLVFGIRGAAWEALEQLGAKLASELPPGT
                     MLHAGICNRFQQSSARAGAVSNVRVLGKSGGAPGSGQAVPIFFATDAATFQRNLLLEE
                     EMFGPATLLVSCGSTEELETIARNLPGQLTATIRGTDEDLARHKNLVRILEQKAGRLI
                     FNGFPTGVEVCPSMHHGGPYPATTAAHFTSVGTAAIQRFARPICFQSFPDAVVPVELR
                     RHNVRKIWRLVDGQFTKDDL"
     gene            4783..5649
                     /locus_tag="VG754_01450"
     CDS             4783..5649
                     /locus_tag="VG754_01450"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWF17900.1"
                     /translation="MDPLISMDAFEIFATGVDHPECIAFDRNGDLWAGGEIGQVYRIA
                     PDGKVRLITTIGGFCAGLAFSPADELFVCNSQHGIVRVNAGGSFEIFASCAGKHKLVC
                     PNYGLFDSAGNYYVTDSGQFRKRNGCLIRFTPDGEGQVLTGPLGYANGLALSADEKTL
                     LMVESDTNSVFRFEIRADGSVGPHSVYAAECGRFPDGLTLDADGNLYICCYASDEIWR
                     IAPSGVKELFAWDPWAVRLGSPTNMAFGGKDFDELYFANLARTTITRVKTGCKGQPLA
                     NQKTATREQASK"
     gene            5630..6379
                     /locus_tag="VG754_01455"
     CDS             5630..6379
                     /locus_tag="VG754_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF17901.1"
                     /translation="MNRLQNKIAIVTGGAHGIGQAIAMAFAQEGATVFMADLDVKAGR
                     ETVAGICKEGGKAFFVRCDVSKISQVRHVVKQAGKNGRIDILCNNAAYIAKVWHAAAD
                     APESEWKKCFEVSLLGSRHFTQAVLPCMLKAGAGSIINISSVQGLVAGRNSAAYTTVK
                     HGLIGFTRSVAYDFGPQNIRCNAICPGAIKTRISPAAGSELHQRQISKTFLGRVGEPR
                     EVASAAVFLASEESSYITGAVLAVDGGWTAM"
     gene            6379..>7257
                     /locus_tag="VG754_01460"
     CDS             6379..>7257
                     /locus_tag="VG754_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17902.1"
                     /translation="MQPTFWKAAAVSVVITPSEPMWLAGWAARREPASGKAGELFCKA
                     LALEDPDGSRVVAVTMDLIAIPRDFAQSVAVEIKKKWNLSRERLFFNSSHTHNGPEIR
                     PDKVPFFEIPAEFAAKIGSYRSQLQENVLSAINIALTNLKPATLNVVQAKADFAQNRR
                     VTGGLSDCDVPILEITGADGKSLAILFGYACHNLTLPFSFCQYHGDYAGVAQQELETH
                     FPGVIAMFFAGAGADQEPAPRGTLELAVQHGRTLADNVKRALQGPKCRVSGNLRIAFE
                     ETPLRFVPLPSPEILAA"
BASE COUNT         1536 a         1926 c         2047 g         1748 t
ORIGIN      
        1 ggcgggactt cttacaccgt gggcgcgaac ctggctggac agaccgatgc ccagggacaa
       61 ggttggtttc aggcaggcag cacaggtcct caaccgatca tcagcgccgg gaatttaacg
      121 atttcaggcc ttgcatcttc cggcggcaac agtgtttctt acggggtgcc tgcgggttcg
      181 cccaccattt ctgccaggtt taatctcggt tcgaccgttg cttcgggaac gctttattat
      241 tccatggctt tgcaagtgac gagccttggc agtttgacga caggcggctc gttcatcgcc
      301 ggtttcaata attccgctgg cgcacaaact gggactccca cgacttttgc cacccgcctt
      361 tacacgcgcc tttcgggaac aggctttaat gtgggtttgg acctcaatgg cacttcatct
      421 acccaatacg gcagcaccac ctataatgtc ggcgacacac tttttatcgt tggcagttat
      481 acttttattt cgggtacggc caatgacacc gcctcgatgt ggctgaatcc ggattcttca
      541 acattcggcg cggtcacggc tccggcgagc actttgactt cggcgagttc tgcggaatct
      601 gacatcgctt caatcgcaag tttcatgctg cgggacggca gcgtagtgga gccgggtctc
      661 attgttgatg atgtccgcat cggaaacaac tgggccgatg tcactcctgc cgttcccgaa
      721 ccggccacga ccatgctggg tggcatgggc ttgggtgtgc tggcgttgat tcgcttggtt
      781 aggtccaagt tgaataaacg ccaactagta agtcggtctt tgctccttag ctaaggcccg
      841 tagattgggc tgatctgggt gcggccggaa tcttcggaca actcataccg gtagatgaag
      901 ttctgttgcg tgatgaattc cgcatgtcca tcgcagaaat tcacgttgtc cccagcggca
      961 ccgtgattgt cgcaggggtc gggatagttt tcgttgccgc cggctaaggg ttgaggttgg
     1021 tccgcgtcca ccatgagcca ggtttgcgat gggccgggaa ccattcccag caggttaaaa
     1081 gcgttcattt cgtgcgcgta ggccaacaca ttcggctctg ttttctttat cggggagcgg
     1141 ccgttgtaac gatggtacca gccatatact tcataacttg ttcccggtac atacttttta
     1201 ctagcggcgg tctgtgtcag atccgtgatt tcattgttaa agatgttggg ccggatgaaa
     1261 ttctgggtgc tggggcagat gtacgtgttc agattcggca cgtaaggcag caggaaattt
     1321 tggtcgtcat cagaaagatt gggctcgctg gtgtagttat gcttttcgtc gtcgttggcg
     1381 tacatctggc agccgagccc gagctgatga aggttgttca tgcaggagat gcgcttggtt
     1441 ttctccttgg ctttcgaaag cgccgggagc aagagggcgg cgagaatcgc gatgatcgct
     1501 atcacaacca gcagttcaat caacgtaaag gcttgtcgtt tcgggttcgg atgcggtttc
     1561 atagtgatga gttactagtt atccgctgat ttgcagcaca aatcctgagt gcgttgaatc
     1621 tatttccgcc aaatgcctaa gtcaatgtat ttcgctactg aaggtatttc gttatatggc
     1681 aatcccggct ggtttgctag acttgcgaca agaagttgag ttactcacag ggtaattgca
     1741 tgttgacagc aatcagcaaa atgggacata ttcgccaaat attattggaa tgtgtttatc
     1801 ttcttaagaa ttaccatgaa aatcaaaaaa gcaacctcgt tcggcctaat cgcttgtatc
     1861 ctctgcgtcg cggctcccat gctagaggcg cttccgttcg ctgagacgtt caacaatgat
     1921 cccgccggca acctaggtgg ccaactcaat gctgaaggga atccttggac gccgatggga
     1981 acgaacggga ccaccggcac tgcggatgtg accgtgacca atttcaacct tgcttatccc
     2041 ggccttgaat gccccggcac caactcggtc ctgtttggct atctggggta cacggagcgc
     2101 gtggccatta atccgcctgt tggcaccaac gccttcggga caactggcgg cacaatttat
     2161 tattcgtatg tgttctatgt gacgaatgtg gcgcagttga cgtcgtccgg ggcgtttgtc
     2221 tctgccttca caaccgtgac gggcacgtcg gccacacagc cgaccgtggg cggcgcgcgg
     2281 ttatacatga aattgagttc catcaaccca agcacggcag tcaacgtggg catcggcaaa
     2341 aatccggctt cgccttcatt tgtcacgtgg gccaccaacg agatcaatgt tggcgatacg
     2401 aatttcattg tggccgccta cacctttttt aacagcgggg catccaatga tgtggcgtcg
     2461 atgtgggtga acccgaattc aaacaccttt ggcgccgtat tttctcctcc gccggacgtg
     2521 accaacacca acggcaccga cgtgcaggtt tccggcgcga accagatcga gagcttctat
     2581 ttacggcagg gcaattccag tattccgctg gtgcttgcga gcaatctacg cctcggctat
     2641 tactggggct gcgtcacgcc tccatccgac ccgggcccga tctcggaggc gaacatgagc
     2701 ctgcttgcgt cgggcacgaa cctgacgctt tcctggggca cgaacgcgcc ttgcttcacc
     2761 ctgcaatcgg cgacatctct ggccagccct ttcacgaatg tggtcccgct ttccgctttt
     2821 actcttgacg cgacgagcaa cgcctatacg aacatcataa cgctccagtc caattcgccg
     2881 accagcaagt tctaccgttt gaagcaagtc gcgaactaat ttgcggtcac ggcatggcgg
     2941 gcgcaaacac ggttttccgc gacgggcatg gaaccttttt cccggccggc aaatggcacg
     3001 acgtttatcg cacttctgag gacgacaacc aaccaacgac ggcaacccga attatccttg
     3061 aatgggaccg cttatcgggt ggttttcaac catggggaca ctccatcgcc gcgaatatct
     3121 tccgttgggc gacttcaaag gagttttggc tttcattttg taccgggctt gaaataaaag
     3181 agccatgcag ttgcacggaa aaaacctcat cggcggcagg atttcaggcc gggggacagt
     3241 ttcattccaa ggagtgatcg ccggttccgg tgaaaaaatc gccccacaat ttttcgaagc
     3301 aacggaagat gaagtggatg aggcgctgcg gctggctgca gaagctttcg aaaagtatcg
     3361 cgttcaacct ccggaaaaaa tagctgcttt cctcgaccag attggtgaag agatcatggg
     3421 tgctggagac gagctgcttc gccgcgcaaa tgctgaaacc gcattacccg aatcgcgtct
     3481 gaccggcgaa cgggcgcgca cggtgaacca atgcaagatg ttcgccgaac tcgtgcgcga
     3541 aggttcctgg gttgaggcgc gcatcgaccg cgctattccc gatcgccagc cgttgccaaa
     3601 gcccttgttg caacggatgc tgatttccat cgggccggtg attgttttcg gcgcgagcaa
     3661 ttttccgctg gcttattcgg ttgcgggcgg cgataccatc tccgctctgg cagccggaaa
     3721 tccggtcgtc gtcaaggcgc atcccgcgca tccgggaact tccgaaattg tggcaggtgc
     3781 agttcaagca gccgccgcag cgacaggaat gccaacagga gttttttcga tgttgcatgg
     3841 acagagcacc gaggttggaa tacgaatggt gcgtcatccg gcggcaaagg cggtcggctt
     3901 caccggttcg ttgcaaggcg ggcgcgcatt gtttgatgcc gccgcggccc gaccggaacc
     3961 aattcccgtt tatgcagaga tgggcagcac caatccggtt ttcatcctgc ccggcgccct
     4021 tgaacgcaat ctggatgcgc tcggccaagg gctcgtgcaa tcggtcacgc tcggcttggg
     4081 gcaattttgc acaaaaccgg gcttggtgtt cggcattcgt ggtgcggctt gggaggcatt
     4141 ggaacaactt ggcgcgaaac tggcttccga gttaccgccg gggacgatgt tgcacgctgg
     4201 catttgcaat cgctttcaac aaagctcagc acgcgcagga gcagtttcga atgttcgcgt
     4261 gcttggcaag tcgggcggcg cgccgggaag cgggcaggcg gttcccattt tttttgcgac
     4321 ggatgccgcc acattccaac gaaacctttt gctggaggaa gaaatgttcg gcccggcgac
     4381 tttgctggtg agttgcggtt cgacagaaga actggaaacc atcgcgcgca atcttccggg
     4441 ccaattgacc gccacgattc gcgggacgga cgaagacctt gcgcggcaca aaaatcttgt
     4501 tcggattttg gaacaaaaag ctgggcggct catcttcaac ggctttccaa ccggtgtgga
     4561 agtctgccct tcaatgcatc acggcgggcc ttatcccgcg acgactgcgg cgcatttcac
     4621 ttctgttggg acggcagcaa tccagcgttt tgcgcgcccg atctgtttcc aaagttttcc
     4681 cgatgccgtt gtcccggtcg aattgcgcag gcacaatgtt cgcaagatct ggcggcttgt
     4741 ggatggccag ttcacgaagg acgatcttta acaatgcgtt tgatggaccc tctcatttcc
     4801 atggatgctt tcgagatatt cgccacgggt gtggatcacc ccgagtgcat cgctttcgat
     4861 aggaatggcg atctttgggc tggcggcgaa atcgggcagg tatatcgcat tgcgcccgat
     4921 ggcaaggtgc ggctcatcac aacaatcggc ggcttttgcg cgggcttggc attttcgccc
     4981 gcggacgaat tgtttgtctg caattcgcag catgggattg tgcgcgtgaa tgccggaggt
     5041 tcgtttgaga tttttgcgag ttgtgccgga aaacataagc tcgtctgtcc gaactatggt
     5101 ttgttcgatt cggcgggcaa ttattatgtc accgattcgg gccagttcag aaaacgcaac
     5161 ggctgtctca tccggtttac tccggatggc gaaggacagg ttctgaccgg gccgctgggt
     5221 tatgcgaacg gactcgcttt atcggcagat gagaaaacgt tgcttatggt ggaaagcgac
     5281 acgaattccg tctttcgttt tgagattcgc gcggatggtt ccgttggtcc acacagcgtt
     5341 tatgcggccg aatgcgggcg ttttccggat ggattgacct tggacgcgga tggcaatctc
     5401 tatatctgtt gttatgcctc cgatgaaatc tggcgcatcg ctccatccgg tgtaaaagaa
     5461 ttgttcgcat gggacccatg ggcggtacgt cttggcagtc cgaccaacat ggccttcggt
     5521 ggaaaagatt ttgatgaact ttattttgcc aacttggccc gcacaaccat aacgcgtgtc
     5581 aaaaccggct gcaaaggcca gccgttggcc aatcagaaaa ctgcaactcg tgaacaggct
     5641 tcaaaataaa atcgccatcg tcactggtgg cgcgcacggc atcgggcagg ccatcgcaat
     5701 ggcattcgcg caggaaggcg cgacggtgtt catggccgat cttgatgtaa aagccggccg
     5761 tgaaactgtc gctggtattt gcaaggaagg cggcaaggcg ttttttgtcc ggtgcgacgt
     5821 ttcaaaaatt tcgcaggtca ggcacgtggt gaagcaggca ggaaaaaatg gacgcattga
     5881 tattttatgc aacaacgccg cttacatcgc caaagtttgg cacgcagccg cggatgcgcc
     5941 cgaatctgaa tggaaaaaat gttttgaagt ttcgctgctc gggagccggc atttcacgca
     6001 agcggttctc ccttgcatgc tcaaggcggg cgcaggttca atcatcaaca tatcttcggt
     6061 gcaggggctt gtcgccgggc gcaattccgc cgcctacacc accgtgaaac acggcttgat
     6121 cggcttcacg cggagcgtcg cttatgattt cgggccgcag aacattcggt gcaacgcgat
     6181 ttgtcccggt gcaatcaaaa cccgcatttc accggcggct ggctctgaac tgcatcagcg
     6241 tcaaatcagc aaaacttttc tcggacgtgt tggcgaacca cgcgaagtgg catctgccgc
     6301 ggtgtttctc gcctcggagg aatcttccta catcaccggc gcagttctcg cagtcgatgg
     6361 cggatggacc gcaatgtgat gcaacccact ttctggaaag ccgccgcagt ttcagtggtc
     6421 atcacgccgt cggaaccgat gtggctggca ggttgggcgg cccgccgcga accagcttcg
     6481 ggcaaagcag gtgaactttt ctgcaaagcg ctcgctctcg aagacccgga cggcagccgc
     6541 gtggtggcgg tgacaatgga cttgattgca attccacgcg attttgcgca gtcggttgcc
     6601 gtagaaatca aaaagaaatg gaatctttcg cgtgagcggc ttttcttcaa ttcatcgcac
     6661 acgcacaatg gccctgaaat ccgtcccgac aaagttccat tcttcgaaat cccggcggaa
     6721 tttgccgcga aaattggatc gtaccgttct cagttgcaag aaaatgtgct cagcgcaatc
     6781 aacatcgcat tgaccaatct caaacctgcg acgttgaacg ttgttcaagc aaaagctgac
     6841 ttcgcgcaaa atcggcgcgt tacaggcgga ctctctgact gtgatgttcc aatccttgaa
     6901 atcactggtg cggatggcaa atcgttggcg attttgttcg gttacgcctg tcacaatctc
     6961 acgctgccat tttcgttttg tcagtatcat ggagattatg cgggcgtcgc gcagcaagag
     7021 cttgaaacgc attttcccgg tgttatcgcg atgttttttg caggcgctgg cgcggaccag
     7081 gaaccggccc cgcgcggcac tttggagctg gctgtacaac atggtcgcac actcgcagac
     7141 aacgtgaagc gagcgttgca aggaccaaaa tgtcgcgtca gtggcaactt gcgtatcgca
     7201 tttgaagaga cgccacttcg atttgttccg ctcccttcgc ccgaaatatt ggctgcg
//
LOCUS       DASZMM010000058         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3317250, whole genome shotgun sequence.
ACCESSION   DASZMM010000058 DASZMM010000000
VERSION     DASZMM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3317250"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VG754_01465"
     CDS             complement(<1..666)
                     /locus_tag="VG754_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF17903.1"
                     /translation="MWIVRLALRRPYTFVVAALVLVLLTPLVLLRTPTDIFPSIDIPV
                     VSVIWQYVGLDAQEIEQRMIYNHERSLSTTVNDIEHMESTSYNGVGIIKVFLQPGSSV
                     DAGVAQITAIAQTILKQMPPGATPPLIIRYNASTVPILQYSVTSDKLSEQELYDMTQN
                     QVRVGLAMVRGTSIPWPYGGRARLVSVDLDLAALKSKSLTPNDVVNAITAQNLILPSG
                     TAKI"
     gene            935..1843
                     /locus_tag="VG754_01470"
     CDS             935..1843
                     /locus_tag="VG754_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWF17904.1"
                     /translation="MELRHLRYFIAVAEELNFRRAAERLHLAQPPLSSQIKDLEEELG
                     TPLFDRSTRSVKLTAAGKIFLEEARAVLLMAQRATQNVRNAHHGLIGPLRIGVLAPSA
                     TPRLARILSAFRKQFPNVHFSLAEFTSTEQLQRLRDDQLDVGLLRPPVTFPELEFLFL
                     ETSPMVLAAPAEHRLAKARKIYWSDFDNEKMVMIHPALQHGYYDKFLELCAKAGSTPV
                     LGQYANDIHSKMWLISAGFGIAPTTKTIAEVKRPGLVFRELPPGLPLVETLLVWKRSN
                     NSPVLRNFLDCFAALAKTCENAPSPR"
     gene            1888..2427
                     /locus_tag="VG754_01475"
     CDS             1888..2427
                     /locus_tag="VG754_01475"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWF17905.1"
                     /translation="MSSKRQIDNIALIGFMGTGKSTVGRLLAEQLRFSFLDTDELIES
                     RAGKTISAIFASEGEAAFRQHEKDVVAELSSRNRVIISTGGGLGASEANIASLKEHAL
                     VVCLWASPEKIWERVRHQSHRPLLQDPDPLAKIRQLLASREKSYKQADALLNTEVRSL
                     KEVAHQVLHQFHLARSGSR"
     gene            2424..>3125
                     /gene="queG"
                     /locus_tag="VG754_01480"
     CDS             2424..>3125
                     /gene="queG"
                     /locus_tag="VG754_01480"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWF17906.1"
                     /translation="MNDAIRQRALELGFDGCRFATAAPPQSADQFQQWLSESKHGEMS
                     WLARNANKRVDPQLVLAGAKSIISLTISYARDEEQKRANPPISQLGTLNPEPGTGIIA
                     RYARFTDYHDVLGEKLKSLANFVDQLGGEKTHSLWYVDTGPVLERDAAQRAGLGFIGK
                     HTNVISRSLGNWIFLAEILTTLQLAPDAPEKNRCGSCASCITACPTNAITAPFQLDAR
                     RCISYLTIEIKGAIPV"
BASE COUNT          672 a          936 c          873 g          644 t
ORIGIN      
        1 gattttcgcg gtgccgctgg gaaggatcaa attttgcgcg gtgatggcgt tgacgacgtc
       61 attgggcgtc aaacttttgg atttcaaagc cgccaaatcg aggtccaccg aaaccaggcg
      121 cgcgcgtccg ccgtaaggcc agggaatgga cgttccgcga accatcgcca acccgacgcg
      181 cacctggttt tgcgtcatgt cgtacagttc ctgctcggaa agcttgtcgc tggtgacgct
      241 gtattgcaaa atcgggacgg tggaggcgtt gtaacggatg atgagcggcg gcgtcgcgcc
      301 cggaggcatt tgcttgagaa tggtctgcgc gatggcggtg atttgcgcca cgccggcatc
      361 cacggaggaa cccggctgga gaaagacctt gatgatgccc acgccgttgt aggacgtgga
      421 ctccatgtgt tcgatgtcat tgacggtggt gctgagcgaa cgctcgtgat tgtagatcat
      481 ccgctgttcg atttcctggg cgtccaagcc gacatattgc cagatgacgc tcaccaccgg
      541 gatgtcaatg gacgggaaga tgtccgtcgg cgtacgcagc aaaaccagcg gggtcagcag
      601 cacaaggacc aatgccgcca ccacgaaggt gtagggacgc cgaagcgcca gacggactat
      661 ccacatatct ttctgtcaaa aaccggcgtt gccgagcgcg gaatttcgtt tccgcctaac
      721 atacccaata ctgttcgtgt gacaaaggca aagcagcaac ttgccattcg ctgcgggggc
      781 agcagtgtgc ggggactgga aagtccccgc aacccgcggc tggaaagctt gcgccacgca
      841 gcgcagccat tcgcccgctc tatttaatcc tcataatgac ccgcgttggc caatatatgt
      901 ttacaacgac tttgatactt tgtatgtatc aatcatggaa ttgcgtcatc tgcgatattt
      961 catagccgtg gccgaggagc ttaacttccg ccgggccgcg gaacggcttc acctcgcgca
     1021 gcccccgctc agctcccaga tcaaggattt ggaggaggaa ctcggcacgc cgctgttcga
     1081 ccggtccacg cgttcggtga aattgaccgc cgccggcaag atatttcttg aagaagcccg
     1141 cgcggtattg ctcatggcgc aacgcgccac gcaaaacgtc cgcaatgcgc atcacgggtt
     1201 gattggtccg ctgcgcatcg gcgtgctcgc cccaagcgcc acgccgcgcc tggcgcggat
     1261 cctcagcgcc ttccgaaaac aatttcccaa tgtgcatttc tcgctggcgg aattcaccag
     1321 caccgagcag cttcagcgcc tgcgcgacga ccagttggac gtcggtttgc tgcgtccgcc
     1381 agtcacgttt cccgaactcg agttcctgtt tttggagaca tcgccgatgg tgcttgccgc
     1441 gccggcggaa catcgcctcg ccaaagcccg caaaatttat tggagcgatt tcgacaatga
     1501 gaagatggtg atgatccatc cggcgttgca gcacgggtat tacgacaaat tcctggaact
     1561 ctgcgcgaag gccggttcca cgccggtgct cggccaatat gccaacgaca tccattccaa
     1621 gatgtggctg atttccgcgg gcttcggcat cgcgcccacc accaaaacca tcgccgaggt
     1681 gaaacggccc gggctcgtgt tccgtgaatt gccgccgggt ttgccgctgg tggagacgct
     1741 cctcgtctgg aaacgatcca acaattcgcc cgtgttgcgc aatttcctcg attgctttgc
     1801 cgcgctggca aagacgtgtg aaaatgcgcc ctcgccgcgt tgaaattact gcatgccaat
     1861 tcgcgatttc tgcggtaagt caaagtcatg agcagcaaac ggcaaatcga caacatcgct
     1921 ctcatcggtt tcatggggac cggcaagtcc accgtcggcc ggctgcttgc cgaacagttg
     1981 cgtttctcgt ttctcgacac ggacgaactc atcgagtcgc gcgcgggcaa gaccatcagc
     2041 gccatttttg cctcggaagg cgaagcggct ttccgccagc acgaaaaaga tgttgtcgcg
     2101 gaattaagtt cgcgcaatcg cgtcatcatt tccaccggcg gcggacttgg cgcgagcgaa
     2161 gccaacatcg ccagcctcaa ggaacatgcg ctggtcgttt gcttgtgggc gtcgcccgaa
     2221 aaaatttggg aacgcgtgcg ccaccaaagc catcgcccgc tgctgcaaga ccccgatcca
     2281 cttgctaaaa tccgccagct tctcgcgtcg cgggaaaaaa gctacaaaca ggccgatgcg
     2341 ctgttgaaca ccgaggtgcg ctcgctgaag gaagtcgcgc atcaggtcct gcatcaattc
     2401 catctcgcaa ggtccggttc acggtgaatg atgccatccg acaacgcgcc ttggaacttg
     2461 gttttgacgg ttgccggttc gccaccgccg cgcctcctca aagcgccgat caattccagc
     2521 agtggctttc tgagagcaag cacggcgaaa tgtcctggct cgcgcgcaac gcgaacaagc
     2581 gcgttgatcc gcagctggtg cttgccggcg caaaaagcat catttcgctg acgatctcct
     2641 atgcgcgcga tgaagaacaa aagagggcca acccgcccat ttcccaactc ggaactctga
     2701 acccggaacc cggaaccgga ataatcgccc gctacgcgcg tttcaccgat taccacgatg
     2761 tcctcggcga aaaattgaaa tcactcgcca attttgtgga ccaacttggc ggcgaaaaaa
     2821 cgcattcact ttggtacgtg gacaccgggc cggtattgga acgcgatgcc gcccaacgcg
     2881 ccgggttggg tttcatcggc aagcacacga atgtgatcag ccgctcgctt ggcaattgga
     2941 tttttctcgc ggagattttg acgacactcc aacttgcgcc cgatgcgccg gaaaaaaatc
     3001 gttgcggttc ctgtgccagt tgcatcacgg cctgcccgac caacgccatc accgcgcctt
     3061 ttcaactcga cgcgcgcaga tgcatttcct atctgaccat cgaaatcaaa ggcgcgattc
     3121 cggtc
//
LOCUS       DASZMM010000059         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3587362, whole genome shotgun sequence.
ACCESSION   DASZMM010000059 DASZMM010000000
VERSION     DASZMM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3587362"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VG754_01485"
     CDS             <1..780
                     /locus_tag="VG754_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWF17907.1"
                     /translation="CRKITFTGSTEVGKSLIQGAANQVKPLSLELGGHAPVIVFDDAD
                     LDAAVEGAMITKFRNTGQSCIASNRIYVQRGIYEKFLGKFVQQTRALKTSDGLEEGVS
                     VGPLINEAGLKQALEHINDATKRGARLLCGGKKMDRKGWFLEPTVLADVPADAVCMSQ
                     ETFAPVAPVCAFDTEAEVIEKANASCYGLSAYAFTRDLKRAFRMMESLEAGTIGINDG
                     VPTTSQCPFGGFKQSGWGRELGVEGLDAFMETKHVSLGLGI"
     gene            803..1834
                     /locus_tag="VG754_01490"
     CDS             803..1834
                     /locus_tag="VG754_01490"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HWF17908.1"
                     /translation="MGSLFNPETSNAEHRTPNAQCHRGVSRFFIRRSVFSVGCSMLSG
                     SSSAFLACLILSFGGAFATAAPLPAEVALNKDAGRMGWWLLPLKMTNGQELLFMVDTG
                     CPITLLDPSLTSKLEKSFDGTLIFNNNLGNRGKSAFYKTPKLLLGGVPLLTGSNCFSF
                     PCDVLSRFSHQKVAGMIGMDCLINYCVQLDFEAGKARFLDAGKLDTNKLGKAFAIEFL
                     QDEGMPADLFTAVVPVVNRPGFLGTKSTRLLLDLGDNVDGGVENGGIQGHYLTRLARF
                     FLPHRALRISQCIWDDQSYTGILLDQGNIRPNRLGIRFFARHLTTFDFPDKTLYLKKI
                     RTGPFPDLK"
     gene            1842..>2867
                     /locus_tag="VG754_01495"
     CDS             1842..>2867
                     /locus_tag="VG754_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17909.1"
                     /translation="MKPDSKIKLPKLGVIFIGRRRPGFDMEWGRQMETRVRNWIAQAG
                     YETAEPAQKPVDDTTLRCAMAELEEENLEAMVVLQTTMGDGRLAPTLAQLWLDPLVLW
                     ATPENQTGDMISSCSLVGAHCWSSVLRQMRHSFELVYGDPDAAMTQKRFHDAIRVAAT
                     VRRLRTARLGLIGGQAPGYFAMGADAFSVHHGLGAQVQTYSLIEFANVVNDLKDEAIA
                     ADVAAVKALKLPHKDTTDEDLPMASRLYLAMRHFFETENLDAMTIRCWPEMPNTFGQW
                     PYLGVARLCEEKRALAVEGDADGALTAWIGEKLGMGRSYLTDWLEHDKETITVWHGGA
                     APMSLCQP"
BASE COUNT          615 a          756 c          848 g          648 t
ORIGIN      
        1 tgccgaaaga tcaccttcac cggctcgacc gaggtcggga aatctttgat tcaaggcgcg
       61 gcgaaccagg tcaaaccgct ttcgctcgaa cttggcggcc atgcgccggt tattgttttt
      121 gacgatgcgg atttggatgc cgcagttgaa ggcgcgatga taacgaaatt ccgcaacacc
      181 ggccagtcgt gcatcgcgtc caatcgcatt tacgtgcagc gcggcattta tgaaaaattt
      241 ttgggaaaat ttgtgcagca aacgcgcgcg ctgaaaacga gcgacggttt ggaagaaggc
      301 gtgtcggtcg gcccgctcat caatgaagcg ggtttgaagc aggcgttgga acacatcaat
      361 gacgcgacga agcgcggcgc ccgtttgtta tgcggcggaa aaaaaatgga ccgcaagggc
      421 tggtttcttg aaccgacggt tttggccgat gtccccgccg acgcggtttg catgtcgcag
      481 gaaacgttcg cgcctgtcgc gccggtttgc gcgttcgata ccgaagccga ggtcatcgaa
      541 aaagccaatg cttcctgtta tggattgagc gcttacgcct tcacgcgcga cctcaagcgg
      601 gcttttcgca tgatggaatc gctcgaagcc gggaccatcg gcatcaacga cggcgtgccg
      661 accacgagtc aatgtccttt cggcggcttc aaacaaagcg gctggggacg cgaactcggt
      721 gtcgagggcc tggacgcgtt catggagacg aagcatgttt cgttgggatt ggggatttaa
      781 gtggcaaagt taaaagacca tcatgggcag tttgttcaat cctgaaacat cgaacgctga
      841 acatcgaaca ccgaacgctc aatgccatcg gggcgtgtcg cgtttcttca ttcgacgttc
      901 ggtgttcagc gttggatgtt cgatgttgtc tggttcttca tcggcattcc tggcgtgtct
      961 cattttatcg tttggcggcg catttgcgac tgcggcacca cttccggccg aagtggctct
     1021 gaacaaggac gccggccgca tgggctggtg gttgctgccg ttaaagatga cgaacgggca
     1081 ggaactgctc ttcatggtgg acaccgggtg ccccatcacg ttgctggatc catctttgac
     1141 atctaaactg gagaaaagct tcgatggcac gctgattttc aataataatc tggggaatcg
     1201 cggcaaatca gcgttctata aaacccccaa gctcctcctg ggcggtgtcc cgctgctgac
     1261 cggatccaat tgttttagtt tcccgtgcga tgttctttcc cgcttttcgc accagaaagt
     1321 ggcggggatg attggcatgg attgcttgat caactactgc gtgcaattgg atttcgaagc
     1381 tggaaaagcg cgcttcctcg atgcgggcaa gcttgatacc aacaagctcg gcaaagcatt
     1441 cgcgatcgaa tttctccaag atgagggcat gccagccgat ttattcacgg ccgtcgtgcc
     1501 ggtcgtgaat cgccccggtt ttttggggac aaaaagcacc cggctattgc tggaccttgg
     1561 tgacaatgta gatggcggcg tggaaaacgg ggggatccaa ggccattatc tgacccgcct
     1621 ggcgcggttc tttcttccgc atcgcgcgtt gcgcatttct caatgtattt gggacgatca
     1681 atcctacact ggcatcctgc tggatcaggg caacattcgt cccaaccggc tcggcatccg
     1741 ttttttcgcg cggcacctca ccacgttcga ttttcccgat aaaactcttt acctgaaaaa
     1801 aatccgcacc ggccccttcc cggatttgaa atgattgatc tatgaagcct gacagcaaaa
     1861 taaaactccc caagctcggc gtgattttca tcggaaggcg acgccccggt ttcgacatgg
     1921 aatggggacg ccaaatggaa acgcgcgtgc gcaattggat cgcgcaagcc ggatacgaga
     1981 ctgccgagcc ggcgcaaaaa ccggtggatg acaccacgct ccgctgcgcg atggctgaat
     2041 tggaggagga aaatcttgaa gccatggtgg tgctgcaaac caccatgggc gacggacggc
     2101 tcgcgccgac gttggcccag ctctggcttg acccgctggt gctatgggcc acgccggaaa
     2161 accagaccgg cgacatgatc agttcctgct cgctcgtcgg cgcgcattgc tggtcatccg
     2221 ttttgcggca gatgcggcat tcgtttgaat tggtttatgg cgatccggat gcggccatga
     2281 cgcagaaacg ttttcacgat gcaattcgcg tggcggcgac agtgcggcgt ttgcgaacag
     2341 cgcgattggg actcatcggc ggacaggcgc cgggttattt cgcgatgggc gcagatgcct
     2401 tttccgttca tcatggtctt ggcgcgcagg tgcagaccta cagtttgatc gagttcgcca
     2461 acgtggtgaa tgatttgaag gacgaggcca ttgccgcaga tgtggccgcg gtcaaggcgc
     2521 tcaaattgcc gcacaaggat accaccgacg aagatttgcc gatggcatcg cgcttgtatc
     2581 tggcgatgcg gcattttttc gagacggaaa atctcgacgc gatgaccatt cgttgctggc
     2641 cggaaatgcc gaatacattc ggacaatggc cttatcttgg cgtcgcgcga ttgtgcgagg
     2701 aaaagcgcgc cctggcagtt gaaggcgatg ccgacggtgc gttaaccgcg tggattggtg
     2761 aaaagctcgg catgggacgc agctacctga ccgactggtt ggaacacgac aaggaaacca
     2821 tcactgtctg gcacggaggc gctgcgccga tgtcgctttg ccaaccg
//
LOCUS       DASZMM010000060         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1938993, whole genome shotgun sequence.
ACCESSION   DASZMM010000060 DASZMM010000000
VERSION     DASZMM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938993"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VG754_01500"
     CDS             complement(<1..1371)
                     /locus_tag="VG754_01500"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF17910.1"
                     /translation="MSMMKQFREPLRLFSPVPALLACMLAMFCGRADAASRQETNTWN
                     EIRNLAADEAVSRTAWAVVETKCEGFIEKFPKSEFVPNVFGIEAQSLFRQGKWDAVIA
                     KFSNAPTNALKMADRLAYWTARSFLEKEDYRAAAENFARLGRDYPASQLRLEAIYREC
                     EAYAKMDEWQRVVEELRESNGAFQQLAKTNSNNQWVVSGSLLLGEAELARKDFSGAKK
                     ILSGIPVQTRPELEWERQFLLCRAKVESGAVKEALLATTNLLTAASGKSEWTAKSIIL
                     KGEIYERLGQLAEAAQTYESNIGGDLPADQRQQALIRIVELTLRQQDKMDEAAQKLSD
                     YLAKYQGEKGSDFEWLMLGELALKKAFLTSQSPTPADQGTNLLQQAEESFLKIKGFTN
                     SAYLGKAELDLGWCYAKQGKTPESIAAFSNAVAQLPFSEDQAVARFKLADTLFLQGDF
                     GGAKTNY"
     gene            complement(1372..>3909)
                     /gene="uvrA"
                     /locus_tag="VG754_01505"
     CDS             complement(1372..>3909)
                     /gene="uvrA"
                     /locus_tag="VG754_01505"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWF17911.1"
                     /translation="QTTSDIVDKILALPPKTRLMLLAPVVRDQKGEFRDVVERLGREG
                     FVRARIDGELVELTNNVRVKLDPKARHTIEAVVDRLVIDDKIRVRLSDSVETALRWGE
                     GRLVMLHQPGGMEQSAAWTETLHSNRNYSPATGQSFEPPTPKHFSFNSPAGACPVCHG
                     LGQKLVFDEGLIVPDTEKSLEQGAILPWRRGGKRMVVYYKSLLRGVAAHYQQNLEAPY
                     KSLPEDFKKVLLWGSGETEIDFNFWRAGKMSTMTRPFEGVVPNLERLYQESESEFTKN
                     RLKGFMSPQFCDACNGQRLKPEILAVTIGGPDVDPKLKIPNSKNAAIPGLSIMEVCSL
                     SVAAADDFFAKLKLTEFQQKIANDVIREIRARLGFLKNVGLGYLTLNRESGTLSGGEA
                     QRIRLATQIGAGLVGVLYILDEPSIGLHQRDNERLLKTLEGLRDLGNSVLVVEHDEDT
                     IRRADYILDLGPGAGVRGGEVVAAGTLDEVLKTPRSLTAKYLTRELAIPVPKKRIKPS
                     PERGWLEVLGARENNLQNIDARIPLGTLTCVTGVSGSGKSTLVDDILRRALFRKFHGS
                     KERPGAHRELRGFENLDKVIVIDQTPIGRTPRSNPATYTGMFNHIRDLFAKLPAARIR
                     GYEAGRFSFNVKGGRCEKCQGDGLIKIEMHFLPPVYVTCEACNGRRYNRETLEISYKG
                     MNIADVLDMTVDEAVNFFRAVPQIYDPCLTLADVGLGYIGLGQAATTLSGGEAQRIKL
                     AAELSRKATGRTLYILDEPTTGLHFHDVAKLLEVLFKLRASGNTLLVIEHNLDVIKTA
                     DWILDLGPEGGAGGGRIVAQGPPEEVARCEGSFTGQYLSRMLAENVR"
BASE COUNT          840 a         1200 c          941 g          928 t
ORIGIN      
        1 ataattcgtc ttcgctccgc caaaatcgcc ttgcaaaaat aaagtgtccg caagcttgaa
       61 tcgagccaca gcctggtcct cggaaaacgg caactgtgcg accgcattgc tgaaagctgc
      121 gatgctctcg ggggtcttgc cttgcttcgc atagcaccag ccaaggtcca gctccgcctt
      181 tcccagataa gcgctgttgg tgaatccctt gatttttagg aagctttcct cggcttgttg
      241 caggagattt gtcccctggt ctgccggcgt tggactctgc gatgtgagaa acgccttttt
      301 caaagccaat tctcccagca tcaaccattc aaaatcagaa cctttctcac cctgatattt
      361 cgcaagataa tctgacagct tttgcgccgc ctcgtccatc ttgtcctgct ggcgaagggt
      421 caattcgacg atgcggatca gcgcttgttg tcgctggtca gccggtaaat caccgccgat
      481 gttcgattca taagtctgcg cggcttccgc gagttgcccg aggcgttcat agatttcgcc
      541 ttttagaatg atgcttttgg ccgtccattc actcttgcct gatgcggctg taagcaagtt
      601 cgtcgtggcc agcaacgcct cttttaccgc tcccgattcc actttggcgc ggcagagcaa
      661 aaactgccgt tcccactcca attcgggccg ggtttgcacg gggattccgg acaagatttt
      721 cttcgcgcca ctgaaatctt tgcgcgccaa ttcggcttca cccagcaaca acgagccgct
      781 tacgacccac tgattattgg aattggtttt tgccagttgt tgaaacgcgc cgttggattc
      841 ccgcagttcc tctactacac gctgccattc atccattttc gcataggctt cgcactcgcg
      901 gtaaatggcc tccaacctca attgtgacgc cggataatcc cgtcccagcc gcgcaaaatt
      961 ttcagcggca gcgcggtaat cctccttttc aagaaacgat cttgccgtcc aataagcgag
     1021 tcgatccgcc attttcaagg cgttggtggg agcgtttgaa aacttggcga tcaccgcgtc
     1081 ccattttccc tgccggaaca acgactgcgc ctcaatgcca aagacatttg gaacaaattc
     1141 tgacttggga aatttttcga tgaacccctc acatttggtt tccacgacgg cccaagcagt
     1201 ccgtgacacg gcttcatcag cggcaagatt gcggatttca ttccaagtgt tggtttcttg
     1261 acggctggcg gcgtcagcgc gcccgcagaa cattgcaagc atgcacgcaa gcaacgccgg
     1321 cacgggcgaa aacaatcgca acggctcccg gaactgtttc atcatcgaca tttatcggac
     1381 attttccgcc aacatacgac tcaaatattg gcccgtgaag ctcccctcgc accgggcgac
     1441 ttcttccggc ggaccttgcg ccacaatgcg tccgccgccc gcgccgcctt ccggccccaa
     1501 atccaggatc caatcggccg tcttgatgac atccagattg tgctcgatta ccagcagtgt
     1561 gttgccggaa gcgcgcaatt taaacaacac ttcgagcagt ttggcaacgt catgaaagtg
     1621 caacccggtg gttggctcat ccaaaatgta gagcgtgcga cccgttgcct tccggctcaa
     1681 ttccgccgcc aatttgatcc gttgcgcttc gccgccgctc aaagtcgtcg ctgcctgtcc
     1741 caaaccgatg tatccgaggc caacatccgc caaggtcaag caaggatcgt aaatctgcgg
     1801 caccgcacgg aaaaaattca ccgcttcatc caccgtcatg tcgagcacgt cagcgatgtt
     1861 cattcctttg taactgattt ccagggtctc gcgattatag cggcgtccgt tgcaagcttc
     1921 gcaggtgaca taaaccggcg gcagaaaatg catctcaatc ttgatcaaac cgtctccctg
     1981 gcatttttcg caacgcccgc ctttcacatt gaaactgaac cggcccgctt cgtaaccgcg
     2041 aatcctcgcc gccggcagtt tcgcgaataa atcgcggatg tggttgaaca tccccgtgta
     2101 tgtggcagga ttgctgcgcg gtgtccggcc aatcggcgtc tggtcaatca cgatcacctt
     2161 gtccaaattc tcaaatcccc tcaattcacg atgtgcgccc ggacgttcct tcgagccgtg
     2221 gaatttgcgg aacaacgccc gccgcaaaat atcatccacc aaagtgctct tgcccgaacc
     2281 gctcacgcct gtgacgcacg tcaacgtccc gagcgggatg cgcgcgtcaa tgttctgcaa
     2341 attattttcc cgcgccccga gcacttcgag ccagcctcgt tccggcgacg gtttgatccg
     2401 ctttttcggg acggggatgg ccaactcgcg cgtaagatat ttcgcggtca acgaacgtgg
     2461 ggtctttaac acttcgtcca aagtgcctgc tgccacgact tctcccccgc gcacgcccgc
     2521 gcccggtccc aaatccaaaa tgtagtccgc gcgccggatg gtatcttcat cgtgttccac
     2581 gacgagtacg gaatttccca aatcgcgcaa gccttcaagc gtcttgagca gccgctcgtt
     2641 atcgcgttga tgcaaaccga tgcttggctc gtccagaatg taaagcacgc ccaccaagcc
     2701 ggcgccaatc tgcgtggcaa gacggatgcg ttgcgcctca ccaccgctca acgtgccgct
     2761 ttcacggttc agtgtgagat aacccagccc cacgttcttg agaaaaccaa gccgcgcccg
     2821 aatttcacgg atgacatcat tcgcaatttt ttgctgaaac tcggtgagct tcaactttgc
     2881 gaaaaaatcg tccgccgcag cgaccgacaa cgaacagact tccatgatgg acaacccggg
     2941 aatcgccgcg ttttttgaat tggggatttt cagtttaggg tcaacatccg ggccgccaat
     3001 ggtgactgcc aggatttccg gcttgagccg ctggccattg caggcatcgc aaaattgcgg
     3061 gctcatgaaa cctttcaagc ggttcttcgt gaactcgctc tcgctttcct gataaagccg
     3121 ttcgagattt ggaaccactc cttcaaatgg ccgcgtcatc gtgctcatct ttcccgcgcg
     3181 ccagaaatta aaatcaatct ccgtttcgcc cgaaccccac aacaatactt ttttgaaatc
     3241 ctccggcaaa cttttgtaag gcgcttccaa attttgctga taatgtgccg cgacgccgcg
     3301 aagaaggctc ttgtaataga ccaccatccg cttgccgccg cgccgccatg gcaagattgc
     3361 gccttgctcc aaagattttt ctgtgtcggg cacaatcagc ccttcgtcaa acacaagctt
     3421 ctggcccaaa ccgtggcaga ccgggcacgc gcccgcgggt gaattgaacg aaaaatgttt
     3481 tggcgtcggc ggctcaaaac tttgtcccgt ggcaggactg tagttgcgat tggaatggag
     3541 cgtttccgtc catgccgccg attgttccat gccgccaggt tgatgcagca tcaccaatct
     3601 gccttcaccc catcgcaatg ccgtctccac cgaatcgctc aagcgcactc gtatcttatc
     3661 gtcaataacc aaccgatcca cgacggcctc gatggtgtgc ctggctttgg gatcgagttt
     3721 aacgcgcacg ttgttggtca gttcaacaag ttctccgtca atccgtgcgc gaacaaaacc
     3781 ttccctgccc aatcgctcga ccacatcgcg gaattcgccc ttttggtcgc gcacaaccgg
     3841 ggcaagcagc atcaaacgcg tcttgggcgg cagcgcaaga attttgtcca cgatgtcgct
     3901 ggtggtctg
//
LOCUS       DASZMM010000061         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4143990, whole genome shotgun sequence.
ACCESSION   DASZMM010000061 DASZMM010000000
VERSION     DASZMM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4143990"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..361)
                     /locus_tag="VG754_01510"
     CDS             complement(233..361)
                     /locus_tag="VG754_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17912.1"
                     /translation="MKRKSDRVLRSEGAPEIGETRSVWSREMDVFVPRRLEDELAV"
     gene            502..774
                     /locus_tag="VG754_01515"
     CDS             502..774
                     /locus_tag="VG754_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17913.1"
                     /translation="MSGGSGTFDVQRSALVVRCFPHWAAVSLGGQTMLFSSILRYSIT
                     PMSPIGVLAYLRLNKKNLRNTSQNDQNDNTAKTLCLLFTFMGFGLN"
     gene            782..2317
                     /locus_tag="VG754_01520"
     CDS             782..2317
                     /locus_tag="VG754_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HWF17914.1"
                     /translation="MPDIVLATLNARYIHAAFGLRYLLANLGELRPRAALLEFDINQR
                     PIEIAETLLAESPRIIGLGVYIWNVAQTTELVSALKRIRPDVTIILGGPEISYETDRQ
                     PVIEMADYVITGEADLKFADVCRGLLAGEKPQQKIIPAELPDLAKLASPYEFYNDRDV
                     AHRIIYVEASRGCPFTCEFCLSSLDIPVRQFPLPSLLQNLEELLQRGVRQFKFVDRTF
                     NLNLNTSRTLLQFFLDRLTPGLFVHFEMIPDRLPEALREIIAKFPPGALQFEVGIQTF
                     NPRVAQLISRRQDYERLADNFQFLRQHTGVHIHADLIVGLPGESEESFGQGFDRLLAL
                     RPQEIQVGILKRLRGTPIVRHDAEWEMVYHEHPPYEVLRTKLINFAEMQRLRRFARFW
                     DLIGNSGNFVTTTPLLWRDAASPFQEFMRLCDCLHAKLGRHHAIPLAQLGEMLFTFLT
                     EQKNLPPQTVALAIWSDYQSGGRSDKPLFLRPYLPATVISRHAHSRGTAPKRQERHLK
                     FQI"
     gene            complement(2334..3668)
                     /locus_tag="VG754_01525"
     CDS             complement(2334..3668)
                     /locus_tag="VG754_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF17915.1"
                     /translation="MLCPVALAFHYWREFSREIDGYPREWFLRWMNRGLLTPLVVWAL
                     MNVGRMPLMPPLYRWIAFLRGNGAWAEAFIAQVCLAGMAMVSIWAVLTFGWFLSSLFK
                     RAKNFEDLIIATVVWSPALLLFWWPFWYVFGWSGSAFGALFWIWPLTHYILNVADLRK
                     PLPTYSQAIAKMKFGKYADAELAIIAQLEKCDTDFDGWMMLADLYATHFHDLAEAEKT
                     ICELCDEPTTTLPQVSMALHRLADWQLNLRADPIAARRVLEEICRRMPGTHLALMARH
                     RINQLPHSVKELEAQHAPKKVIMPAWVEELDAQKPAAPLNTEDAMALVNQFVEELKKD
                     PNDILPREKLARVFAGHMGKVDLAVEQLELLIGMPGQSPAKTAEWLGLMATWLLEQHG
                     ESEAAQKILERLIHEFPQSPQAFAAQRKLIVIRTKPRGRIKERTYEHEDEDE"
     gene            complement(4044..4574)
                     /locus_tag="VG754_01530"
     CDS             complement(4044..4574)
                     /locus_tag="VG754_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17916.1"
                     /translation="MKSKSCFLFLAAIFFAAAPLRADSPTTFNVGEFTFTRPAKWEWV
                     EVTSSMRKAQLKVIDADGKASADVVFFQFGPQAGSVKANVDRWLRQFDPSGKEPKIEE
                     TTIGKTKVTYVEAEGTYHSGMPGGPLTPMPDSALMGAIMESEDGNVFVKMTGPKALVK
                     ASIADFKKMIESGPKK"
     gene            4704..5414
                     /locus_tag="VG754_01535"
     CDS             4704..5414
                     /locus_tag="VG754_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWF17917.1"
                     /translation="METGEAGSGFFIWGVDQSAYGPVELPTLVNWIRDDRVVADTWIF
                     AQQSAHWQPAAKIPELQMFFHGRSSRGASSSLNSFPGHVRPSALRRVKILANLGDDQL
                     ERFAQLVDFEKVAPWTAIVKKGEHGDSMYLILEGEARVRLMEGHRETILATLGPGEFF
                     GDISLFDHGPRSADVIANTDTTVAKISAENFEKLSIEAPDLAVLFLTATVKTLAARIR
                     ADNKRLSHSVASAQAVEA"
     gene            5573..>5879
                     /locus_tag="VG754_01540"
     CDS             5573..>5879
                     /locus_tag="VG754_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF17918.1"
                     /translation="MGMRQNSIARESAGEIPRRALGATGEKVSMLGLGGYHIGKPSEE
                     DSLRIVRTAVDAGVNFMDNCWDYNDGNSEVRMGKALRDGYRSKVFLMTKIDGRDKQTA
                     "
BASE COUNT         1286 a         1744 c         1506 g         1343 t
ORIGIN      
        1 ggcggttgat gctccgcaag ctggaggctt gcaagaaatt agccgggggt aaggccgcag
       61 gccgccaccc ccggttgtct gttgttcaat tttccaccct gaaagggtgg cagaagcgca
      121 attggttcgt tggttctggc atcccttcag ggtgcatcgg tttacgcgac attttccggg
      181 ggtgtcgctt cgctcaaccc ccggctaatt tcttgcaacc ctccgggttg cttcagacag
      241 ccagttcgtc ttcgagacgc cgcgggacga aaacatccat ttcgcgcgac cagacactgc
      301 gtgtctcacc aatctccggt gctccttcgg agcgtaaaac gcggtcgctt ttgcgcttca
      361 ttcgtaagtg gttgaattcc cggataattt cagataattc cagacagttt cagataattc
      421 cgccaaaaat acccccctcc cccccatgcg aaccgaaaca tcgaacgccc aacatcgaac
      481 gtcgaacact gaatcaggga aatgtcgggc ggctctggaa cattcgatgt tcagcgttcg
      541 gcgttggttg ttcgatgttt tccgcattgg gcagccgtgt cgcttggcgg tcaaaccatg
      601 ctcttttcat caatactccg ctactccatt actccaatgt cccccatcgg cgtactcgct
      661 tatttacgct taaataaaaa aaatttacga aatacctccc aaaatgatca aaatgacaac
      721 accgccaaga ccctttgttt gctgttcact tttatgggtt ttggcctaaa ttgaccctgc
      781 catgccggac atcgtgctcg caacgctcaa cgccaggtat atccacgccg ccttcggttt
      841 gcgttatctg cttgctaatc tcggcgaact ccgcccgcgc gcggcgctgc tcgaattcga
      901 catcaaccag cgacccatcg agattgccga aaccctcctt gccgaatcgc cgcgcatcat
      961 cgggctggga gtctatatct ggaacgtcgc gcagaccacg gaacttgttt ccgctctcaa
     1021 acggattcgc cccgatgtga ccatcatctt gggcggtccc gaaatcagct acgaaaccga
     1081 ccggcagcct gtcatcgaaa tggcggatta cgtcatcacc ggcgaagctg atttgaagtt
     1141 tgcggatgtt tgccgcggat tgctggccgg agaaaagccg cagcaaaaaa tcattcccgc
     1201 cgagcttccc gatttggcca aactcgcgtc tccctacgaa ttttataacg accgcgatgt
     1261 cgcgcatcgc atcatttacg tcgaggcctc gcgcggctgt ccgttcacgt gcgaattttg
     1321 cctttcgtcg ctcgacattc ccgtgcgcca gtttccactg ccatctctgc tgcaaaatct
     1381 tgaggaactg ctccagcgcg gcgtccggca attcaaattt gttgatcgca ctttcaacct
     1441 gaacctcaac accagccgga cgctcctgca attctttctg gatcgcctga ctccgggatt
     1501 gttcgtccat ttcgagatga ttcccgaccg cctgccggaa gcgttgcgcg aaatcatcgc
     1561 caaatttccg ccgggcgcgc tgcaattcga ggtgggcatc cagactttca atccgcgcgt
     1621 cgcacagctc atcagccgcc ggcaggatta cgagcggctt gccgacaatt tccagttttt
     1681 gcgccagcat accggcgttc acatccatgc cgacctcatc gtcggccttc ccggagaatc
     1741 cgaggaaagt tttggacagg gattcgaccg tttgctcgcg ctgcgtccgc aagaaattca
     1801 ggttggcatt ctcaaacgac tgcgcggcac gcccatcgtg cgccacgatg ccgaatggga
     1861 aatggtttat catgagcatc cgccttacga ggtgttgcgc accaagctca tcaactttgc
     1921 cgaaatgcag cgtctgcggc ggttcgcaag gttttgggac ttgattggca atagcggcaa
     1981 ttttgtgacc accactcccc tgctctggcg cgacgccgct tctccgttcc aggaattcat
     2041 gcgcctctgc gattgtttgc acgcgaagct cggacgccat cacgccatcc cgctcgcgca
     2101 actcggtgaa atgcttttta cattccttac tgaacaaaaa aatctgccgc cacaaaccgt
     2161 cgcgttagcc atctggtccg attaccaatc cggcggacgc agcgacaaac cgttgtttct
     2221 ccggccctat ttgcccgcga ccgtcatttc gcgccacgct cattcccgcg gcacagcccc
     2281 caaacgccag gaacgccatc tcaaatttca gatttgattt tcattatgcg caactattca
     2341 tcctcatctt cgtgttcata cgttcgttcc tttatccggc ctctcggttt tgttcgtatc
     2401 acgatgagct tccgttgcgc ggcgaaggct tgcggagatt gtggaaattc atgaatcaat
     2461 cgttccaaaa tcttttgcgc cgcctcgctt tcgccatgct gctccagcaa ccatgttgcc
     2521 atcaagccga gccattccgc cgtttttgcc ggggattgtc ccggcatccc gatcaacaat
     2581 tcgagctgtt ccaccgcgag gtcaactttt cccatgtgcc ccgcgaaaac gcgggccagc
     2641 ttttcacgcg ggaggatgtc attgggatct ttcttgagtt cctccacaaa ttggttcacc
     2701 agcgccatcg cgtcctcggt gttcaacggc gcagccggtt tctgcgcgtc gagttcttca
     2761 acccacgcgg gcatgatgac ttttttgggc gcgtgttgcg cctccaattc cttgaccgaa
     2821 tgcggcagtt ggttgatgcg atgccgcgcc atcaaggcga ggtgcgtgcc gggcatccgg
     2881 cggcaaattt cttccagcac ccggcgggcc gcgattggat cagcgcgcaa gttcagttgc
     2941 caatccgcca gccggtgcag cgccatggaa acttgcggca acgtcgtcgt cggttcatcg
     3001 cacaattcgc agatggtttt ttccgcctcg gccaaatcgt gaaagtgggt cgcatacaag
     3061 tctgcgagca tcatccagcc gtcgaaatca gtgtcgcatt tttccagttg cgcgatgatg
     3121 gccaattcgg cgtcggcgta tttgccgaat ttcatttttg cgatggcctg cgaataagtc
     3181 ggcagaggct ttctcaaatc ggcgacgttc aagatataat gcgtcaacgg ccagatccaa
     3241 aacagcgcgc caaacgcgct tccgctccat ccgaacacgt accagaacgg ccaccaaaac
     3301 aacaaaaggg ccgggctcca aaccacggtc gcgatgataa ggtcttcgaa gttcttcgcg
     3361 cgcttgaaca gactcgaaag gaaccagccg aaagtgagca cagcccagat ggagaccatc
     3421 gccattcccg ccaggcacac ttgcgcgatg aatgcctcgg cccatgcacc gttgcctcgc
     3481 aggaaggcga tccatcggta caatggcggc atcaggggca ttcgtccgac gttcatcaac
     3541 gcccagacga caagcggcgt cagaagcccc cggttcatcc agcgaaggaa ccattcgcgc
     3601 gggtatccat ctatttcgcg ggaaaattcc ctccaataat ggaacgccag cgccaccgga
     3661 cacagcatca agcagatgga aacaaaccaa agcataccgg gtgtcggaac ttggctcacc
     3721 ctagcctcgc ggctgaaaaa ttccaagctg cttatggaga tggaagaggc tgtaacgctc
     3781 cttttggaga aaaaagaaaa aggcggcccc actcagggcc gccgaaccta actgaccatc
     3841 attacggaac tattaatcaa aatgtggccc tgcgtttgta tggggtgttc accccaacga
     3901 tggttttaaa aatgcaaaac tcgaatctat ttccgtcaat tttttgccgg ggcattctcc
     3961 ctattggacg ctgccctgtg ggaaggcacg ttaaaacaaa gctaacatta aaaaactccc
     4021 gcttccaagg agggcgggat tctctacttc ttcggaccgc tctcaatcat cttcttgaaa
     4081 tccgcgatgg atgctttgac gagcgctttg gggccggtca ttttcacaaa gacattccca
     4141 tcttcggact ccatgattgc gcccatcaac gcggaatcgg gcatcggcgt gagcggcccg
     4201 ccgggcattc cgctgtgata agtgccttcg gcttccacgt aggtcacctt ggttttgcca
     4261 atcgtcgttt cttcaatctt cggctccttg ccgctcggat cgaactggcg cagccagcgg
     4321 tccacgttgg ccttgacgct tccggcctgc gggccgaact ggaagaacac aacgtccgcg
     4381 ctggcctttc catcggcgtc gatgactttc aactgcgcct tgcgcatgga cgacgtcacc
     4441 tcgacccact cccatttggc cggacgagtg aaggtgaatt cgccgacgtt gaaagtcgtt
     4501 ggcgaatccg cgcgcaaagg cgcggccgcg aagaaaattg ccgccaaaaa aagaaaacag
     4561 gattttgatt tcatataatg aaaaaaggat cgcgaactct atttacgcct caaggcacga
     4621 ccgcgcaaga ggttttgaac accgtcgaaa aaacttgcac ttttcatttg tcattttttc
     4681 gtgaggtgtt atacaggcgg gatatggaaa cgggagaagc tgggagcggc tttttcattt
     4741 ggggagttga tcaatcggct tacgggccgg tcgaactgcc aacgctcgtc aactggatac
     4801 gggacgaccg cgtggtggcg gacacctgga tcttcgccca acaaagcgct cattggcagc
     4861 cggcggcaaa aattcccgaa ctccagatgt ttttccatgg cagatcttcc cgtggcgctt
     4921 cgtcttcgct caatagtttt cccgggcacg tccgtccgtc ggcattgcgc cgcgttaaaa
     4981 tcctggcgaa cctgggcgac gaccagctcg aacgtttcgc gcaattggtg gatttcgaaa
     5041 aagtggcgcc ttggacggcc atcgtgaaaa aaggcgagca cggcgattcg atgtatctca
     5101 tccttgaagg cgaggcgcgc gtgcgtctca tggaaggcca tcgggaaaca atccttgcca
     5161 cattgggccc gggcgaattt ttcggcgaca tctccctttt cgaccatggc ccgcgttcag
     5221 ccgacgtcat cgccaacacg gacacaaccg tcgcaaaaat ttccgcggag aacttcgaga
     5281 aactttcgat tgaagcgccc gaccttgccg ttctctttct caccgcaacg gtcaagacgc
     5341 tcgccgcccg catccgcgcc gacaacaaac gtctcagcca ttctgttgcg agtgcgcaag
     5401 cggtcgaagc gtgaagatcg gcaccctgcc ttgacaaccc gcagtggccg gccgcaaatt
     5461 gaattcaacg gcacgtttgc cgcgtcacat tgaaaagcga ggttttatgc aaacaacaaa
     5521 ttccatcacg cggcgcagat ttctccaggt caccggcacg acggctttgg tgatgggcat
     5581 gcggcaaaat tccattgctc gcgaaagcgc tggtgaaatc ccacgccgcg cgctcggcgc
     5641 gactggcgaa aaagtttcga tgcttggcct cggcggttac cacatcggca agccaagcga
     5701 ggaagacagt ctccgcatcg tccgcacggc cgttgatgcg ggagtcaact tcatggacaa
     5761 ctgctgggat tataatgacg gtaacagcga agtgcggatg ggcaaggcgt tgcgggacgg
     5821 ttatcgctcg aaagtttttc tgatgacgaa gattgacggt cgcgacaagc agaccgccg
//
LOCUS       DASZMM010000062         7478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_839764, whole genome shotgun sequence.
ACCESSION   DASZMM010000062 DASZMM010000000
VERSION     DASZMM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839764"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VG754_01545"
     CDS             complement(<1..738)
                     /locus_tag="VG754_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HWF17919.1"
                     /translation="MKPHPKIIDRILKEINTHRTFCVVGHVRPDGDCIGSQLAVTLAL
                     REQGKKVTCWNEDPMPDKLKFLDADKLVQRPKPGASFDCVIATDSASFERLGKTGACI
                     SHRKLLINIDHHASNTRYGDINWVSPKEASTGEIIFRFMKAVNVPITPAIADCLFTAV
                     STDTGSFQYPSTRPSTYNVAGELVKRGADLAKICQEVYQSYPLSRAKLLKHVYNKFRL
                     THDNQIAYFWLKQADYTRTGAKPDDSEG"
     gene            complement(713..1090)
                     /gene="rbfA"
                     /locus_tag="VG754_01550"
     CDS             complement(713..1090)
                     /gene="rbfA"
                     /locus_tag="VG754_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWF17920.1"
                     /translation="MSSLRLERVRELLKRELGEIIRREIPVGEAGLITVNDVHVASDL
                     HSAMVYVGVLGSAGQQKKALAGLEHHRKRIQGLVGKAVILKYTPQLRFMIDQSIEEGN
                     KVLKIIDELEHTAPPDEATPKDH"
     gene            complement(1113..3614)
                     /gene="infB"
                     /locus_tag="VG754_01555"
     CDS             complement(1113..3614)
                     /gene="infB"
                     /locus_tag="VG754_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWF17921.1"
                     /translation="MPVRIYDISKKLGLENKEVLAKAKELGIAAAKVPSSSLDKITAE
                     FLETELTKGRPNAAPPPPPTPVVAEHKINIVKAPPPPEPEVPVEMPPPPVIAEVPPAA
                     IPEMEQPKENGEAVTAATPEPSTITPPAPAAPPPPSGPQVGDKIGFIQLPSKPAPKAG
                     DKIGSVKLPPSRPGAPAPTTGRQDFGRGRPGDNRNFRGGIPPTQGRPQPPGAFNKPAG
                     LAPRQASPTAPKFVAPDTGLVITIKPPIIVRELAEQLKRKPFQLIADLMELGVFATVN
                     QAIDEEIAKKVCAKHGYRFEVEKRERGSGIVHAPVKKVELDIEDKTEDLRPRAPVVTI
                     MGHVDHGKTTLLDVIRKSNVVAGEAGGITQHIGAYTISFPHPERKNELQQITFLDTPG
                     HAAFSSMRARGANVTDIVVLVVAANDGVMPQTLEALSHAQAAHAEILVAVNKVDHPNA
                     NPMKVRQQLQEKGLVCEEWGGKTLFVDVSALTKQGVDKLLEAVLLQAEIMELKANPNR
                     RAKGNVIESGLEPGGPTATVLVRKGTLRLGDIVICGQYYGKVRALINEEGKREKEAGP
                     SVAVKLLGLNGVPEAGLEFSVVEDEKEARDLAETRAMEARALGHQEARAKVTLENLFA
                     TMTSNAGKVLKLVVKADTQGSVEAIVEALNKIESDKVSLEVIHSAVGTITESDVSLAS
                     ASDAVILGFHTRIDNGVSDVAKREGVQIKLYAIIYELIDQVKEALAGLLDPILKDVVI
                     GSAEVRKIFNLSKGSPVAGCMVTTGRIIRGKVRVMRRKGLIYEGVTQTLRRFQDEVNE
                     VRAGMECGIRIEGFGDFQPGDTIECYSVERVAQKL"
     gene            complement(3717..5003)
                     /gene="nusA"
                     /locus_tag="VG754_01560"
     CDS             complement(3717..5003)
                     /gene="nusA"
                     /locus_tag="VG754_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWF17922.1"
                     /translation="MNADFLAVLEFWEREKGISRDILVAAVEESLLSAAKKAVGPARE
                     LRCVIDQKSGDIKAFAKLVVSDKVLSKHDQISVFDARRIKPDAQLGDELEVEVTPAGF
                     GRIASQYAKQSLMQHIRRAEKQLIFTEFKDRVGDIISGVVRRFDRSDVSVDLGKYEAL
                     LPNRERVPTEEYQIGERIRCYVKAVENGPHGPEIILSRADPRFVIKLFQLEVSEINDG
                     TIEIKGIAREPGFRTKLAVYTRDEKVDPVGACVGLRGQRVKNIVRELNNEKVDIIRWD
                     SNIKVFLTNALAPAQLKSFEVDDASRRVRILVSEDQLSLAIGKRGQNARLSSKLTGWQ
                     VDIEPEVVITMGFEEKVAQAVKTLAAIPGLTQQQADVLVHHGFTRLEDLLQADASDFS
                     GIPEIGEQAGAIFDAVRAEAARRTLKVGDAGEASPA"
     gene            5268..5639
                     /locus_tag="VG754_01565"
     CDS             5268..5639
                     /locus_tag="VG754_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17923.1"
                     /translation="MTQPLALIFYEKLLPGTQLVNRMQDLGYRVVTVTGESLVASAAH
                     EKPMLVLADLTARRTNVVEAISQLRANPETNHLPVIAFAEDKDAALQSAARAAGATLV
                     VNETAIDTHLEQFLEQALRLD"
     gene            5741..6556
                     /gene="hemQ"
                     /locus_tag="VG754_01570"
     CDS             5741..6556
                     /gene="hemQ"
                     /locus_tag="VG754_01570"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF008913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HWF17924.1"
                     /translation="MTLPTVKLNQGLHVLHLFYRVDRARWTQLPAGESAQSRAKLEKL
                     CAANANPSHPRLVTYANISGKADFAFMLQAAELGQAAQLHRDLEACFAPGVLVPVFSY
                     LSVTELSEYMPTEEESRQMLIQQEKLSPDSEAFKTKMAESNKRRADYEHYRLYPELPD
                     WEIMAFYPMSKRRLGNDNWYSLDFDTRKKLMGGHARVGRKFAGRVSQLITGSIGLDGW
                     EWGVTLMAHQLDAVKEIVYEMRFDEVTARYGEFGPFFINMRMDAATLWQHLHL"
     gene            6574..7242
                     /locus_tag="VG754_01575"
     CDS             6574..7242
                     /locus_tag="VG754_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HWF17925.1"
                     /translation="MQKPRHEIICPRLTNFKFDDFDSVSGMFEGIPAIAMQQAWLPAL
                     EADFAPASVRTGWRDEALFVFAELTDADIFTRATRHNERLWELGDVFEIFLRPDAQQA
                     YTEFHIAPNNLRLQLRFADTEALKRLAAAKSLENAMIDAQLFESAVWVRPEEQRWHVS
                     AKIPAASICEAKAPMQGSLWHFSFSRYDYTRGREKPVISSTSPHAIARFHSQAEWGTL
                     RFSP"
BASE COUNT         1535 a         2148 c         2087 g         1708 t
ORIGIN      
        1 tccctcggaa tcatcgggct tcgcgccggt gcgcgtgtaa tcggcctgct tgagccagaa
       61 ataagcgatc tggttgtcat gcgtgaggcg aaatttgtta tagacatgct tgagcaattt
      121 cgcgcgggaa aggggatagg attgatagac ttcctggcag atttttgcga ggtcggcgcc
      181 gcgtttcacg agttcgccgg cgacgttgta agtgctcggg cgcgtgctgg gatattgaaa
      241 agagccggtg tcagttgaaa cggcggtgaa caggcaatcc gcaatcgccg gtgtgatggg
      301 cacgttgaca gccttcatga agcggaaaat gatttcaccg gtggatgctt ctttcgggga
      361 aacccagttg atgtcgccat agcgcgtgtt gctcgcatga tgatcaatgt tgatcagcaa
      421 tttccggtga ctgatgcacg cgccggtctt gccaagccgc tcgaaacttg ccgaatcggt
      481 ggcgatgacg caatcgaatg acgccccggg tttgggcctt tgcaccagtt tgtcggcatc
      541 cagaaacttg agcttgtcgg gcatcgggtc ttcgttccaa cacgtgactt ttttgccctg
      601 ctcccgcaag gcgagcgtca cggccagttg cgagcctatg caatcgccat ccgggcggac
      661 gtgtccgacc acacaaaacg tgcggtgggt attgatttct ttgaggatgc ggtcaatgat
      721 ctttgggtgt ggcttcatca ggaggcgcgg tgtgttccag ttcgtcaatg atcttgagga
      781 ctttattgcc ctcttcgatc gattgatcaa tcatgaaacg caactgcggt gtgtatttga
      841 gaatgacggc ctttccgacg aggccttgaa tgcgtttgcg gtgatgttcc aagccggcga
      901 gcgctttttt ttgctgcccg gcgctgccca gcacgccgac atagaccatg gcggaatgca
      961 gatcgctggc gacgtggaca tcgttgacgg tgatgagacc agcttcgccc acaggaattt
     1021 cgcgccggat gatttcaccg agttcgcgct tgaggagttc gcgcactcgt tcgagtctga
     1081 gcgaggacat gagaaaaaac gctttctaaa aatcaaagtt tctgggccac gcgctcgacg
     1141 gaatagcatt cgatcgtgtc gcccggttga aaatctccga agccttcgat gcggatgccg
     1201 cattccatgc cggcgcgcac ttcgttgacc tcatcctgga accggcgcaa cgtctgtgtg
     1261 acaccttcgt agatgagacc cttgcgccgc atgacgcgga ccttgccgcg aatgatgcgc
     1321 ccggtggtga ccatgcaacc ggccacagga ctgcccttcg agagattgaa aatcttgcgc
     1381 acctcggcag agccgatgac gacatctttg aggatgggat cgagcaatcc ggcgagggcc
     1441 tccttcacct ggtcgatcaa ttcgtaaatg atggcgtaaa gcttgatctg cacgccttcg
     1501 cgcttggcaa catccgagac gccattatcg atgcgcgtgt gaaatccaag gatgactgcg
     1561 tccgaagccg aagccagcga aacgtcagat tcagtgatgg tgccgacggc actgtgaatg
     1621 acttcgaggc tgaccttgtc cgactcgatt ttgttgagcg cttcgacaat cgcctcgacg
     1681 gagccctgcg tatcggcctt cacgacaagc ttcaggactt tcccggcgtt ggacgtcatc
     1741 gtggcgaaaa gattttcgag cgtgaccttg gcgcgggctt cttggtggcc aagcgcacgg
     1801 gcttccatgg cgcgggtttc ggccagatcg cgggcttctt tttcgtcttc aaccacgcta
     1861 aattccagac cggcttccgg cacgccattc aagccgagca atttcaccgc gacggacggc
     1921 ccggcttcct tctcgcgttt gccctcttcg ttgatgagcg cgcggacttt gccataatac
     1981 tggccgcaaa tcacaatgtc gccgagacgc aatgttccct tgcgcacgag cacggtggct
     2041 gtggggccgc ccggttcgag gccggattca atcacgttgc ccttggcgcg gcgattgggg
     2101 ttggctttca actccatgat ttccgcctgg agcaaaaccg cttcgagcag cttgtccacg
     2161 ccttgtttgg tcaatgcaga cacatccacg aacaaggtct tgccgcccca ttcctcgcaa
     2221 acgaggccct tttcctgaag ctgctggcgc accttcatcg gattggcgtt gggatggtcc
     2281 accttgttga ctgcgacgag gatttcggcg tgggccgcct gcgcatggct caacgcctcc
     2341 aaagtctgcg gcatgacgcc gtcattcgcg gccacaacca gcacgacgat gtccgtgaca
     2401 ttcgcgccgc gcgcgcgcat ggagctgaat gcggcatggc cgggcgtgtc gaggaacgtg
     2461 atttgttgca gctcgttttt tcgttcggga tgcgggaatg aaatcgtgta agcgccgatg
     2521 tgctgggtga tgccgccggc ttcgcctgcg acgacattcg atttgcggat gacatccagc
     2581 agtgtggtct tgccatggtc aacgtggccc atgatggtca cgaccggcgc gcgcggccgg
     2641 aggtcttccg tcttgtcctc gatatccagc tcgaccttct tcaccggggc atggacgatg
     2701 ccgctgccgc gttcgcgttt ttcgacttcg aaacgataac cgtgcttggc gcaaactttc
     2761 ttggcaatct cctcgtcaat cgcctggttc accgtggcga aaacgcccag ttccatcaga
     2821 tcagcgatca actggaacgg cttgcgcttg agttgctcgg cgagttcccg cacgataatg
     2881 ggcggcttga tggtgatgac caaacccgta tcgggcgcga cgaatttggg cgctgtcggc
     2941 gacgcctgtc tgggcgcaag accagcaggt ttattgaacg ctccgggcgg ctgcggcctg
     3001 ccttgagtcg gcggaatgcc gccgcgaaaa ttgcgattat caccgggacg tccgcggccg
     3061 aaatcctgac gtcctgtcgt cggcgcggga gcgccgggac gcgacggagg caatttgacc
     3121 gagccgatct tatcgcctgc cttgggcgcg ggcttgcttg gcaattgaat gaaaccgatc
     3181 ttgtcgccga cttgcggccc tgaaggcggc ggcggcgctg ccggggcagg cggcgtgatg
     3241 gttgaaggct cgggagtcgc ggccgtcaca gcttcgccat tttccttggg ctgttccatt
     3301 tctggaatgg cagcaggagg aacttcggcg atgacaggtg gcggaggcat ttcgactgga
     3361 acttcgggtt caggcggcgg cggggccttg acgatgttga ttttgtgttc ggcgacgaca
     3421 ggtgttggcg gcggcggcgg ggcggcgttg ggccggcctt tggtcagttc ggtttcgagg
     3481 aattcagcgg tgattttgtc gagcgagctt gaaggtactt tggcggcggc aatgcccagt
     3541 tctttagcct ttgcaagaac ctccttgttc tcgaggccga gtttctttga aatatcgtaa
     3601 atacgaacgg gcataatcta gttctattct ctctctatct accgacaatt tttgcaaaca
     3661 agccttcata ggctttttaa tttaacacaa gcgcaattca tttgccatgg ccgccgtcac
     3721 gcgggagaag cctcgccggc gtcgccgacc ttgagtgttc tccgcgccgc ttccgcgcgc
     3781 acggcatcga aaattgcgcc tgcttgctcg ccaatttccg gaatccccga aaaatcgctt
     3841 gcatccgcct gcaacagatc ttccaaccgc gtaaaaccgt gatgcaccag gacatccgcc
     3901 tgttgctgcg taaggccggg aatcgccgcc agcgtcttca ccgcctgcgc gactttttcc
     3961 tcgaagccca tcgtgatcac cacttctggc tcgatgtcca cctgccaacc ggtcagcttg
     4021 gaagacaacc gggcgttctg tccgcgcttg ccgatggcca gtgatagctg gtcttcgctc
     4081 accaaaatcc tgactcggcg gctggcatcg tccacttcaa aagacttcaa ttgcgcgggt
     4141 gccaacgcgt tggtcaggaa caccttgatg ttagagtccc aacggatgat gtccaccttc
     4201 tcgttgttca attcgcgcac gatatttttg acccgctggc cgcgcaaacc gacgcatgcg
     4261 ccgacaggat caaccttttc gtcgcgtgta taaaccgcga gcttggtgcg gaaaccgggc
     4321 tcgcgcgcga tgcccttgat ctcaatcgtc ccgtcgttga tttccgacac ttccaactgg
     4381 aacaacttga tgacaaagcg cggatcggcc cgcgacaaaa tgatttccgg cccgtgcggg
     4441 ccgttctcga cggccttcac gtagcagcgg atgcgctcgc cgatctggta ttcttccgtc
     4501 ggcacacgct cgcggttcgg caacagggcc tcatatttgc ccaggtccac gctgacgtcc
     4561 gaacggtcaa accgccgcac gacaccgctg atgatgtcac ccacgcggtc cttgaactcg
     4621 gtgaaaatca actgcttttc cgcgcgacgg atgtgctgca tcaacgattg cttggcgtat
     4681 tgcgatgcga tgcgcccgaa accggccggt gtgacttcca cttccagttc gtcaccgagt
     4741 tgggcatcgg gtttgatgcg acgcgcgtcg aacacggaaa tctggtcatg cttcgacaat
     4801 accttgtccg agacgaccag cttggcaaag gctttgatgt cccctgattt ttggtcaatg
     4861 acgcaacgca gttcacgcgc cggaccgacg gctttctttg cggcagacaa aagcgattcc
     4921 tcgacagcag cgacaaggat atcacggctg atacccttct cccgctccca aaattcaagt
     4981 actgctaaaa agtctgcgtt cataaaatcg tgttcggcac ccctcggcag ccagcaaagg
     5041 gaacaaaaaa gtcggcagat tttatcttcc gactttggcg cgctggcact gaaccagcca
     5101 atctataccg ttgcaataaa cataacgact tgcgggacgg cgtcaaggtt caaatcagag
     5161 gggttaatcc tttataattc aagcttttac cacacaaaaa ccgggcattt ggatgttccc
     5221 atctaaaact ttttgcaatt tgccctcatc tgcctaaaac tctctccatg acgcaaccgc
     5281 tcgctctgat tttttacgaa aaattactgc ccggcaccca attggtaaat cgcatgcagg
     5341 acttgggtta ccgtgtcgtg accgtcacgg gcgaatcgtt ggttgcttcc gctgcccatg
     5401 aaaaaccaat gcttgtcctg gccgacctga ccgcgcgccg gacgaatgtc gttgaagcca
     5461 tttctcaact tcgggcaaac cccgaaacca atcatctccc ggtgatcgcc tttgccgagg
     5521 acaaggacgc cgcccttcaa tccgccgccc gcgccgccgg cgcaaccttg gtggtcaacg
     5581 aaaccgccat cgacactcat ctcgaacaat tcctcgaaca agcgctacgg ctggattgat
     5641 aaatcgctgt gaacgctgaa cttccaacat cgaactccga acatcgaagg attcgacgtt
     5701 cgatgttcag cgttggttgt tcgatgttat ctaaaatttt atgacgcttc cgaccgtaaa
     5761 actcaatcaa ggcctccacg tcctgcatct gttttatcgc gttgaccgcg cgcgatggac
     5821 gcaactgccc gccggcgagt cggcccaaag ccgcgccaaa ctggaaaagc tttgcgccgc
     5881 caacgcgaac ccgagccatc cgcggcttgt cacttacgcg aacatcagcg gcaaggcgga
     5941 ttttgcgttc atgttgcagg cggcggaatt gggccaggcg gcgcaattgc atcgcgacct
     6001 tgaagcctgt ttcgcgcccg gcgttctcgt cccggtgttc agttacttga gcgtcaccga
     6061 gttgagcgaa tacatgccaa ccgaggaaga aagccgccag atgctcatcc agcaggaaaa
     6121 actttcgcct gacagcgaag ctttcaagac caagatggcc gaatccaaca aacgccgcgc
     6181 cgattatgaa cattaccggc tctatcccga attgcccgat tgggaaatca tggcgttcta
     6241 tccgatgagc aagcgacgcc tgggaaatga taattggtat tcgctcgatt ttgatacccg
     6301 caagaaactc atgggtggcc acgcgcgtgt gggacgtaaa ttcgccgggc gcgtctcgca
     6361 actcatcacc ggctcgattg gtctggacgg gtgggaatgg ggcgtcacgc tcatggcgca
     6421 tcaactcgat gccgtaaagg aaatcgttta tgaaatgcgg ttcgatgaag tgaccgcgcg
     6481 ttatggtgaa ttcgggccat tcttcatcaa tatgcggatg gatgccgcca cgctctggca
     6541 gcatttgcat ctttgatgcc cgaaaaccag attgtgcaaa aacccaggca cgaaatcatc
     6601 tgcccacgat tgacgaattt taagttcgac gatttcgatt ccgtcagcgg catgtttgaa
     6661 ggcatcccgg cgattgccat gcagcaagcg tggttgcctg cgctggaagc ggatttcgcc
     6721 ccggcgtccg tccgaaccgg ctggcgcgac gaagcgcttt ttgttttcgc cgaattgacc
     6781 gatgccgaca ttttcacgcg cgccacacgg cacaacgaac ggctatggga attgggcgac
     6841 gtctttgaaa tctttcttcg ccccgatgcg cagcaagcct acaccgaatt tcacatcgca
     6901 cccaataacc ttcggttgca actccgcttt gccgataccg aagccctgaa acgtcttgct
     6961 gcggccaagt ctctggaaaa cgccatgatt gacgcgcagc tttttgaatc cgcggtttgg
     7021 gtccggccgg aagagcaacg atggcatgtc tccgcaaaga ttcccgccgc ttccatctgc
     7081 gaggcgaaag cgccgatgca aggcagcctc tggcattttt ctttcagcag atatgattac
     7141 acccgcggac gtgagaagcc ggtgatttcc tccacgtcac cccacgccat tgcgcgattc
     7201 cacagccagg cagagtgggg gacattacgt ttttcaccct aagccgtaac gattcagatg
     7261 aaaggggagc gcacgcctct ggcgtgcagt gtccggcgtc ccgccaaaca cttcgtaaaa
     7321 ctgattttct gtccaccaaa cagtgctcga aaaccaaaag taaaagtcct cgcccggacg
     7381 gctcgggccg cacgcaagac gcgtgcggtc cctttttcgt cggcatgatt ccggctaaga
     7441 tgatcatcta aatagtagag cgcggctgtc cgcaaacc
//
LOCUS       DASZMM010000063         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1939998, whole genome shotgun sequence.
ACCESSION   DASZMM010000063 DASZMM010000000
VERSION     DASZMM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939998"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /gene="larC"
                     /locus_tag="VG754_01580"
     CDS             <1..508
                     /gene="larC"
                     /locus_tag="VG754_01580"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HWF17926.1"
                     /translation="NVLRAILGETVTSSLTTSANDWETDTIAVLETNLDDINAEILGD
                     FVNRALTEGALDVFHTPVQMKKNRPGVLLTVLCASADADKFTELMLRETSAFGVRRST
                     AERRKLHREFVTVKTEFGEVTVKLGNLNGKIVQATPEYESCKKVAEQAKVPLKAVYEA
                     AQKSLKTG"
     gene            516..761
                     /locus_tag="VG754_01585"
     CDS             516..761
                     /locus_tag="VG754_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17927.1"
                     /translation="MDAELLKILCCPETHQELRLAAPELLDKINAQIAAKRLQNRAGR
                     VVEEKIDGGLVRADGRFLYPVHEQIPVMLVDEALPLN"
     gene            761..1417
                     /locus_tag="VG754_01590"
     CDS             761..1417
                     /locus_tag="VG754_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17928.1"
                     /translation="MKAVCPKCGREIPMEDVNVEKDIALCRSCNQTFSFADLVQESRE
                     PAIDLARPPKGVWFKQDMRGFEIGSTTRSPVAFFLVPLMCLWSGGSLARIYGSQIRSG
                     KFEIGKSLFGLPFLLGTIVLGTITLMTIFGKTTVSVQGDQGKVFVGLGPIGWTRRFKW
                     SGVREIREGYCGTTRNGRQQMGIILEGETRLTFGSGMREERRYFIFSAMRNLLHKREG
                     "
     gene            complement(1437..1772)
                     /locus_tag="VG754_01595"
     CDS             complement(1437..1772)
                     /locus_tag="VG754_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWF17929.1"
                     /translation="MTRLKIARVDEVPEGGTVKFPFMHKGRHAEGFVARFQGKLVAYE
                     NRCQHLPVRLDFPDAKLFNRDGTHFICQNHNAIYEPSTGLCVRGPCEGQTLRALEIQI
                     VQGEVWVLV"
     gene            1908..3107
                     /locus_tag="VG754_01600"
     CDS             1908..3107
                     /locus_tag="VG754_01600"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWF17930.1"
                     /translation="MKNWLNSIESVIADLRKGRMAIVVDDEDRENEGDLIMAGEFATP
                     EAVNFMARFGRGLICVPTTSERLKQLGIEQMVTRNRESFKTDFQVSVDAAKGITTGIS
                     AADRARTIQIMASPTALPNDLVQPGHVFPLRAKPGGVLQRSGHTEAVVDLVQLAGCRP
                     IGVLCEIMNDDGTMARLPQLIKFARKHKLKICTIEALIKYRREREKLVERIEVVKMPT
                     DYGEFDLYLYRSKLDGQHHLALVRGDVSGKKDILVRVHSECLTGDVFGSRRCDCGPQL
                     HQAMRMVSEAGRGVVVYMRQEGRGIGLAPKIQAYKLQEQGYDTVEANKKLGYGMDLRE
                     YGLGAQILADLGIKHIRLLTNNPKKVVGLEGYGLEIVEQIPIKIRPNPHNEHYLKTKR
                     EKLGHLL"
     gene            3162..3434
                     /locus_tag="VG754_01605"
     CDS             3162..3434
                     /locus_tag="VG754_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17931.1"
                     /translation="MKVLSVSGAKAKLGRVIDQVIKTREPVVIPRGSRHVVIAPYDLP
                     TPTEIELARIGQEMDRSGVAVEESEESLQAIRKAVRKYRAERRDRQ"
     gene            3446..3892
                     /locus_tag="VG754_01610"
     CDS             3446..3892
                     /locus_tag="VG754_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HWF17932.1"
                     /translation="MVPATQPMMVVMDTNVFISAVGSRRGNAWRCFILFARRRFRLAV
                     TDQVLREYETIASRMAGKPGIFHGMNWRPLYDWVCNEAEYFEPAQLGKRRSRDADDDV
                     YLACALSASASILVSYDADLLDLEKPFGIQILKPAAFVRLMNRVSP"
     gene            3889..4275
                     /locus_tag="VG754_01615"
     CDS             3889..4275
                     /locus_tag="VG754_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF17933.1"
                     /translation="MKILALDHIAIHVKDLEASCRFYSEVLLAQPLPRPAFDFPGAWF
                     RLGANQELHLIGGRDQSVASHSRGNHFALETDDLIGWEHHFKKIGFQHRARKLRPDGA
                     GQIFLEDPDGHVIELCAKNGIAKQHA"
     gene            4268..4747
                     /gene="ribH"
                     /locus_tag="VG754_01620"
     CDS             4268..4747
                     /gene="ribH"
                     /locus_tag="VG754_01620"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWF17934.1"
                     /translation="MLKSNRSKNFKARGGQFVIIASKYNARYVDAMLKSAQSELKRAG
                     AAKVKIVRVPGAFEIPTIATKLANASHKTSDSRIAAFICLGVILRGQTVHAEHIGRAV
                     TEALMWIQVRYEVPVIHEVLLLENEEQARVRCLGTKYNRGTEAAQTALEMARVMAEL"
     gene            4829..5533
                     /locus_tag="VG754_01625"
     CDS             4829..5533
                     /locus_tag="VG754_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF17935.1"
                     /translation="MKMNPRFLVIPALVVGLQAATAADITGTITLNGKPPEEKIIDMS
                     SIADCSKQHPEPVKTQFYVVGPKGELKDVIISIKNLSGKSTGESAEPLVLDQKGCEYS
                     PYVTAVQTGQKILVKNSDPVFHNVDVIPTKEGNKPANKAQGPGAPDIAMSFSAEESFL
                     RFKCDVHPWMFAYVSIIDNPYFAVSDKDGKFKIANVPAGKYTLEAAHRKAGKVTKEIE
                     VKADGAVVDFTMELPK"
BASE COUNT         1340 a         1436 c         1578 g         1214 t
ORIGIN      
        1 caacgtgctg cgcgcaattc tgggtgaaac tgtgacgagt tccttgacta cctccgcgaa
       61 tgattgggaa accgacacca tcgcggtcct tgaaaccaat ctggatgaca ttaatgcgga
      121 aatactcggc gattttgtca accgggcgct gaccgaaggg gcgttggatg tttttcacac
      181 gccggtccag atgaagaaaa accgcccggg cgtgttgctc accgttttgt gcgcgtcagc
      241 ggatgcggac aaattcaccg aattgatgct tcgcgaaacc agcgcgttcg gtgtgcgccg
      301 ttcgacagcc gagcgccgga aactccaccg cgaattcgtc accgtaaaaa ccgaattcgg
      361 tgaagtgacc gtcaagcttg gcaatctcaa tggaaaaatt gtgcaggcca cgccggaata
      421 cgagtcgtgc aaaaaagtgg ccgaacaggc caaagtgccg ttgaaagcag tttacgaagc
      481 ggcgcagaaa tccttgaaga ctggatagca ctgacatgga cgcggagttg ttgaaaattc
      541 tttgctgccc ggagacgcac caggaacttc gcctggccgc gccggagttg ctggacaaaa
      601 tcaacgcgca aattgccgcc aagcgtttgc aaaatcgcgc tggccgcgtc gtggaggaaa
      661 aaattgatgg cggcttggtc cgtgccgatg gacgttttct ttatcccgtc catgaacaaa
      721 ttcccgtgat gctcgttgac gaagcgcttc cgttgaactg atgaaagctg tctgccccaa
      781 atgtggccgt gaaatcccga tggaagatgt caacgtggaa aaagacatcg cgctttgccg
      841 ctcttgcaac cagacattca gctttgccga cctggtgcag gaaagccggg aacccgcgat
      901 tgatctggct cgaccgccaa aaggcgtctg gttcaaacaa gacatgcgtg gatttgaaat
      961 tggctcgacc acgcggtcac cggtggcttt ttttctggtg cctcttatgt gcctgtggtc
     1021 gggaggctca cttgccagaa tttacgggtc gcaaatccgt tccggcaaat tcgagatcgg
     1081 caagtccctg tttggacttc cattcttgct cggcacgatt gtcctgggaa ccatcacttt
     1141 gatgacgatt tttggcaaga cgaccgtttc cgtgcagggt gatcagggaa aagtgtttgt
     1201 cggtctgggg ccgatcgggt ggactcgccg gttcaaatgg agcggcgtcc gggaaatccg
     1261 cgaaggatat tgcggcacga caagaaatgg ccggcagcag atgggcatca ttttggaggg
     1321 cgaaacgcgg cttacttttg gttctggaat gagggaagaa cggcgatatt ttattttcag
     1381 cgccatgcga aatttgcttc ataaacggga aggttaagcc acttcccgtt gagaaatcaa
     1441 accaacaccc aaacttcacc ttggacaatt tgaatctcca gagcgcgcaa agtttgacct
     1501 tcgcacgggc cgcgaacgca aaggccggtc gaaggttcgt agatcgcgtt gtggttctgg
     1561 caaatgaaat gggtcccgtc gcgattgaag agctttgcgt ccggaaaatc caatcgcacc
     1621 gggagatgct ggcaacgatt ttcataggcg acgagtttgc cttgaaaccg cgccacaaaa
     1681 ccttccgcgt gtcggccttt gtgcatgaaa ggaaacttta ccgtcccgcc ttccggcact
     1741 tcatccacgc gagcaatttt gagcctcgtc acgcgaagag cttattaaga atgccggcac
     1801 gagacaacca ccaagacaca aagtcaccaa gaaggtcttc ctctttatgc ctcggtgaat
     1861 tttttgttgc tggaatttat cgcatggccg gttagcttct catcgctatg aagaactggc
     1921 tgaactcgat tgaatccgtc attgccgatt tgcgcaaggg acggatggcg atcgtggtgg
     1981 acgatgaaga ccgcgaaaat gaaggcgatc tcatcatggc cggtgaattc gccacgcccg
     2041 aggcggtcaa tttcatggcg cgatttggcc ggggattgat atgtgtcccc acgacttccg
     2101 aacgcctcaa gcaactcggc atcgagcaga tggtgacgcg caaccgcgag agtttcaaaa
     2161 ccgattttca agtcagcgtg gatgccgcca agggcatcac cacgggcatc agcgccgccg
     2221 accgcgcgcg caccatccag atcatggctt cgcccaccgc gctgccgaat gatttggtcc
     2281 agccgggtca cgtttttcct ttgcgggcca agccgggtgg cgtgctgcaa cgttcgggcc
     2341 acacagaagc cgtggtggac cttgtccaat tggcgggttg ccgtcccatc ggggtgctgt
     2401 gcgaaatcat gaacgatgac ggcacgatgg cgcggttgcc gcagttgatc aagttcgccc
     2461 gcaaacacaa actgaaaatc tgcaccatcg aggcgctcat caaataccgt cgtgaacggg
     2521 aaaaattggt cgagcgcatc gaagtggtga agatgccgac cgactacggc gaatttgatt
     2581 tgtatcttta ccgctcgaaa ctggacggac agcatcatct tgccttggtg cgcggagacg
     2641 tttcagggaa gaaagacatt ttggtgcgcg tccatagcga atgtttgacg ggcgatgtgt
     2701 tcggttcgcg ccgttgtgat tgcggcccgc aattgcacca agccatgcgg atggtgtcgg
     2761 aagcgggccg cggcgtggtg gtttacatgc ggcaggaagg gcgcggcatc gggctcgcgc
     2821 cgaaaatcca ggcgtataaa ttgcaggagc agggttacga cacggtcgag gcaaacaaaa
     2881 aactcggtta cggcatggac ctacgcgaat acggccttgg cgcgcaaatc cttgccgacc
     2941 tcggcatcaa acacatccgt ctgctcacga ataatccgaa gaaagtggtt ggactggaag
     3001 gatatggttt ggagatcgtc gagcaaatcc cgatcaaaat ccgccccaat ccgcacaacg
     3061 agcattacct gaaaacgaaa cgcgagaagc tggggcatct attgtgaaac gccggactta
     3121 aatacattga cagtggtcca caaatggtcc atttttggac tgtgaaagtt ttgagcgtaa
     3181 gcggggccaa agccaaactt ggccgggtga ttgatcaggt catcaaaacc cgggagccgg
     3241 tggtcatccc gcgtggttcc cgtcatgtgg tgattgcacc gtatgatttg ccgacgccca
     3301 ccgagatcga actggcgcgt atcggccagg aaatggacag gagcggagtg gccgtagaag
     3361 aaagcgagga atctttgcaa gccatccgca aggcggtcag aaaataccgg gctgagcgcc
     3421 gggatcggca ataattccca tttaaatggt tcctgcaacc cagccgatga tggtcgtgat
     3481 ggacacgaac gtcttcattt ccgcggtggg ctccagaaga ggcaatgcat ggcggtgttt
     3541 catcttgttc gccaggcgac gatttcgcct cgcggtcaca gaccaggtgc ttcgcgaata
     3601 tgagacgatt gccagccgca tggccggaaa gccagggatt tttcacggga tgaattggcg
     3661 gccattatat gactgggtgt gcaatgaggc tgagtatttt gagcccgcac aattaggcaa
     3721 acggcgcagt cgcgacgccg acgatgatgt ttatctcgca tgcgcgctct ccgccagcgc
     3781 aagcattctt gtttcatacg atgccgactt gctggacctg gaaaaaccgt ttggaatcca
     3841 gattttgaag ccagctgcgt ttgtgcgttt gatgaaccgg gtttctccat gaaaattctc
     3901 gctttggacc acatcgccat ccatgtgaaa gatttggagg cgagttgcag gttttactcc
     3961 gaggtgctgc tggcgcaacc gttgcctcgc cctgcattcg attttcccgg cgcgtggttt
     4021 cgactcggcg cgaatcagga gctgcatctg atcggcggtc gcgatcaatc cgtggcttca
     4081 cacagccgtg gcaatcattt cgcgctggag acggatgatc tgatcggatg ggagcaccat
     4141 tttaaaaaaa tcggttttca acaccgagcg cgcaagctgc ggcctgacgg cgcgggccag
     4201 atttttcttg aagacccgga cgggcacgtc atcgaattgt gcgcgaaaaa cggcatcgca
     4261 aaacaacatg cttaaatcca accggtcgaa aaatttcaag gcgcgcggcg ggcaatttgt
     4321 catcatcgcc tccaaataca atgcgcgcta tgtggacgcg atgttgaagt cggcgcaatc
     4381 ggagttgaaa cgcgcgggcg cggcaaaggt gaaaattgtt cgtgttccgg gcgcattcga
     4441 gattccaacc atcgcaacga aactggccaa tgccagccac aaaacatcgg attcgcggat
     4501 agcggctttc atctgccttg gcgtcatatt gcgcggacag acagtccatg ccgaacacat
     4561 tggtcgcgcg gtgacggagg cattgatgtg gatacaggtg cgctatgaag ttcctgtcat
     4621 tcacgaagtg ctgctgttgg aaaatgaaga gcaggcgcgt gtgcgctgcc ttggcacaaa
     4681 gtacaatcgc ggcactgaag ccgcgcaaac ggcgttggaa atggcaaggg tgatggccga
     4741 attgtaacct tgtgaaaact ttcacatact gcttgcacgc tggataagtg aatgttattt
     4801 tgctcacgtt tttcggaact aataaaatat gaaaatgaat cctcgctttt tggttattcc
     4861 ggctttggtc gtcggattgc aggcggcaac ggctgcggac atcactggaa ccatcacctt
     4921 gaacggcaaa ccgccggaag aaaagatcat agacatgagt tccattgcgg attgctccaa
     4981 gcagcacccc gaaccggtca agactcagtt ttacgtcgtc gggccgaagg gggaactgaa
     5041 ggatgtcatc atctccatca aaaatctcag cggcaaatcc actggtgaat ccgccgagcc
     5101 gcttgttttg gaccagaaag gctgcgaata ctcgccttac gtcaccgcag tccagaccgg
     5161 ccagaaaatt ttggtcaaaa attccgaccc ggtgtttcac aacgtggacg tcatcccgac
     5221 caaagaaggc aacaagcccg ccaacaaagc ccagggtccc ggcgcgcctg acatcgccat
     5281 gagtttctcc gctgaggaaa gctttctccg cttcaaatgc gacgttcacc cttggatgtt
     5341 cgcttatgtg tccattattg ataatcctta cttcgcggtc agcgacaagg acggcaagtt
     5401 caagatcgcg aatgttccag ccggcaaata cacgctggaa gccgcgcatc gcaaggccgg
     5461 caaggtgacc aaggaaatcg aggtcaaggc cgacggcgca gtcgtggact tcacgatgga
     5521 actgcccaaa taatttttta aactcgagcg ccgctgcggc aaattgac
//
LOCUS       DASZMM010000064         7909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_568050, whole genome shotgun sequence.
ACCESSION   DASZMM010000064 DASZMM010000000
VERSION     DASZMM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568050"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..853)
                     /locus_tag="VG754_01630"
                     /pseudo
     CDS             complement(5..853)
                     /locus_tag="VG754_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(869..1189)
                     /locus_tag="VG754_01635"
     CDS             complement(869..1189)
                     /locus_tag="VG754_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17936.1"
                     /translation="MSDLQDILRGEIDREGAISFARFMELSLYCPKIGYYEQSGVSPG
                     RKGDFYTSVSVGELFGDLLANQFAEWLAPEAQSQISNLKSQINDPTSHVGAYCGDNGK
                     WQIV"
     gene            1254..2348
                     /locus_tag="VG754_01640"
     CDS             1254..2348
                     /locus_tag="VG754_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWF17937.1"
                     /translation="MAKIDAFFNLMFEQKASDLHVSSGNPPMLRINGELHRVDYPPLE
                     SDALKLMLYEIAPEYKIKLFEETGDVDFGYEIPNISRFRANFFNQKNGVSAVFRQIPS
                     RVLSFEDFEKFDAPLPAVLKKLSMLHKGLVVVTGPTGSGKSTTLAAMVDYANKNRRDH
                     IITVEDPIEFVHESKNCLVNHREVGVHTKSFSAALRGALREDPDIILVGELRDLETIE
                     LAITAASTGHLVFGTLHTQSAGKTVDRIIDVFPSEQQNKIRATLSEALKGVVAQNLFK
                     RVDKKGRVAALEILVFTTAIANLVREGKTHQIPGMIQVGKKHGNQPLDDAIMEHLRMK
                     RISPEEAYDKCLDKKKFRTFLANPPDEDET"
     gene            2361..3572
                     /locus_tag="VG754_01645"
     CDS             2361..3572
                     /locus_tag="VG754_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWF17938.1"
                     /translation="MRRPELDSILSTMLDAAPEVSDLLFTVDKPLQVEAAGELTPVES
                     EPPLVRLTPYQTEVIALNLIGENIWQIKDLLRTGSCDAAYTVMDKARFRINIFSQRGN
                     YSIVLRKLNTKIPTLESLKLPEIINQIPREKTGLVLVTGATGSGKSTTLAAVLSEFNR
                     TKAIHVITLEDPIEFVHPHDKATFNQRELGTDYDSFANGLRAALRQAPKVILVGEMRD
                     KETVKIALSAAETGHLVLSTLHTVDAGQTVNRILGMFETEEQEQMRLRLADTLRWVVS
                     QRLVPKIGGGRWALLEIMGSNLRTKETIVQGESEGKTFYEIIESSQSFGWRTFDSSAV
                     EAYDQGVITEESAILYCSKRGPVSRAIDNLKKSRGESTTQLNGLRMKAPVNTGNGSKP
                     SPPIPATLKLK"
     gene            3575..4045
                     /locus_tag="VG754_01650"
     CDS             3575..4045
                     /locus_tag="VG754_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17939.1"
                     /translation="MSDEFDFIGAADKPALLAFSTPDWLEMARGALLELGYKIHTAAT
                     HSDFLIRFTQVRYQVVIIEELFAANTLEENLTLINVQNMPMNQRRHAAVILLGNSFQT
                     FTPMQAFQFSVSAVINASEIFLLKQLVEKSVADHTFFFHSFREAQSSLFRQATA"
     gene            complement(4136..4645)
                     /locus_tag="VG754_01655"
     CDS             complement(4136..4645)
                     /locus_tag="VG754_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17940.1"
                     /translation="MTLKEIIPELEQLDRGLTIYAADPWHASTKALVARPLDDGRVPE
                     EAVTAGCGYFLEVFVALGIVHHLSASAASEPTEDEKCAKLIEYAQWVSSDARSPQQTP
                     FKWNFPVSMMIYCSACGKHFDIVIAGKGAGHYHCSVCGKVHEFDLEAFAQGAIEQSRK
                     MRKRKHGHR"
     gene            complement(4669..5805)
                     /locus_tag="VG754_01660"
     CDS             complement(4669..5805)
                     /locus_tag="VG754_01660"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWF17941.1"
                     /translation="MKNVVLLGSTGSIGTSTVKVADDLPDKIRLVGLGAGNNLELLLE
                     QTRKHQPTAISITDPAKAKEARNALGTSTEVYSGTDGLIKLATMPSADIVLIAIVGTA
                     GLQPALAAIRAGKDIAVASKEILVMAGEIVMSEARKHGVRVLAVDSEHSAIFQCLEEK
                     PGHSVRNLWLTASGGPFRTTPKEEFAAITVERALKHPSWVMGRKITIDSATLFNKGLE
                     MIEARWLFDIEMGRVKVVVHPQSVVHSMVEFVDGSIIAQLSTPDMCLPIQYALTYPER
                     ARSDRVQTNLAKLGTLTFEEPDVDRFPSLNLARQAGEIGGTLPAVLNAANEVAVEAFV
                     NRRITFTQITDTVRRTMERHQVTSHPTLEQILAADSWARAEAAR"
     gene            complement(5827..6186)
                     /locus_tag="VG754_01665"
     CDS             complement(5827..6186)
                     /locus_tag="VG754_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HWF17942.1"
                     /translation="MTREEMMLRTKTYANRVVRLCQALPNNWLARTLGGQLLRSGTSV
                     GANYRAVCRAKSTADFINKLRVVEEECDESLFWMEILVDNSLIRESRLRSLMQEGHEI
                     LAIIVSSAKTARSSRRA"
     gene            complement(6231..7151)
                     /locus_tag="VG754_01670"
     CDS             complement(6231..7151)
                     /locus_tag="VG754_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HWF17943.1"
                     /translation="MPNETIAVAPALSKRQIFFRRLFSFVMLWTIILAGLFSGNKLIS
                     DLVFLVVIVLLACSGLWEFYGLVEKRDLVCFKYSGLMGGSLLMIGTFLNLTGVVGTHN
                     YLPSRVNDFETSFLILFVLGLCVRQFVSKSNTAGILAISTTLFGLMYVPWLLNFIQKI
                     NFFPGINGHFYLLYFLLITKFSDMGAYSVGSLIGKHKMIPRISPGKTWEGFGGAIVVS
                     TVASVLFVHFAHDEMPAMNWKHAVVLGVVLSISAVIGDLIESLFKREAGVKDSGHLFP
                     GIGGILDLLDSLLFNAPLMYIYLRHVIMHP"
     gene            complement(7202..7702)
                     /locus_tag="VG754_01675"
     CDS             complement(7202..7702)
                     /locus_tag="VG754_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3806 domain-containing protein"
                     /protein_id="HWF17944.1"
                     /translation="MKKTLLFVLVTMLFVDLSIAQNPKNMSQTSSPKFAELSKEDAAR
                     LEKQRHLIAVIAMQKYGAPALTKTKSDLPILQRILDDKVFKKSQTYELQCLGITFGDV
                     LASEFPLRWVMVTDEYGTDPTLRFKETTWQINALTMISKRVEKGEIVNLAELVRLTGE
                     YVKKYQ"
     gene            complement(7699..>7909)
                     /locus_tag="VG754_01680"
     CDS             complement(7699..>7909)
                     /locus_tag="VG754_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HWF17945.1"
                     /translation="ADHLYTRKWPDPDVLIRTSGEMRVSNFLLWQISYAELVVTQTLW
                     PDFRKPQFITALEEYAQRHRRFGGI"
BASE COUNT         1879 a         2246 c         1998 g         1786 t
ORIGIN      
        1 acgatcaccg cgcttccact cccctgccct gcaccagcac ctgaaacgat tgccccaaat
       61 gctcaggatg cgtgagcgtt tggaattggc ggacgcgcga tggagtccat tccttgacgg
      121 tgggattgtg gcagcaattc ttttcaatga tgtgagtcag gaattgccgt tgcgtgatga
      181 agctctcggt cttcaatccc tccgcttcgc cggctgattg gatcgcggtg aaattgatat
      241 gcgcggtaat gtcttgttgg ccgggacgcg ctagcaaatc ttgagtttga tggtgctgat
      301 gataggcgca caatgtgcct tgctttcttt cgggcgtaaa aaattgttca gccttcaatc
      361 cgtaatcaag cgtcatcagc ttgccaaact ttaatgctct cgctgcccga cgccaccaat
      421 ccatcgcggc aggacaaact tcggtcgtaa aaccgtccgg caacactgcg agcaattcct
      481 gcggcagatc gccgaagaat tcttccattt ttaattttgc atttttaatt ttaaattccc
      541 tggtccagac aaattgagtt ccgtcccagg tcacgcccca ttcgaaccat tttttcttgg
      601 cggcgtccca accgagccga tgcaccggca tggcgtccaa aagttcattc gagaatatga
      661 tgccatttac gcccgatgcc gggacgtgct cccatgattc aaaccagcgc actgaaccgg
      721 cgaattcggc gagcgttttt tcctgcatct gcctgcgttg cggcgaaggc tccaaaatcc
      781 agtattccaa ccgcgcgaaa agctccctcc gctccgtccg caaccaccgc aaaatatccg
      841 ccgccaatcg cgcgtcatgc gcgcccgcct agacgatttg ccacttaccg ttgtcgccac
      901 agtaagcgcc gacatgagaa gttggatcgt taatttgaga tttgagattt gagatttgag
      961 attgcgcctc cggtgccagc cattccgcga actggttcgc cagcaggtcc ccgaacaatt
     1021 cccccacgct gacgctcgtg tagaagtccc ctttgcgccc cggcgataca cccgattgct
     1081 cgtagtatcc aatttttgga caatacagtg acagttccat gaagcgcgcg aaggaaatgg
     1141 ctccctcgcg gtcaatctct ccccttagaa tgtcctgcaa atcgctcatt gcacaattca
     1201 tgccgcctgt aaccgcctct gggcacgatt actgctaata tatcgtaggt tttatggcca
     1261 aaatagacgc gttcttcaac ctgatgttcg agcagaaagc ctcggacctt catgtgtcat
     1321 ccggcaatcc gccgatgctc cgcatcaatg gcgagctgca tcgcgtcgat tatcccccgc
     1381 tggagagcga cgcgttgaag ttgatgctct acgaaatcgc cccggaatac aaaatcaaac
     1441 tcttcgaaga aaccggcgac gtggacttcg gctatgaaat cccgaacatc tcccgtttcc
     1501 gcgcgaattt cttcaatcag aaaaatggcg tttccgcggt cttccgccaa attcccagcc
     1561 gcgtgctgtc gttcgaggat ttcgaaaaat ttgacgcccc gctgcccgcc gtgctcaaga
     1621 agctttccat gctgcacaag ggtttggtcg ttgtgaccgg cccgaccggt tccggcaagt
     1681 ccacgacgtt ggccgcgatg gttgattacg cgaacaaaaa tcgccgcgac cacatcatca
     1741 ctgtcgaaga cccgattgaa ttcgtgcacg aaagcaagaa ctgcctcgtg aaccaccgcg
     1801 aagtgggcgt gcatacgaag tcgttctccg cggcattgcg cggcgccctg cgcgaagacc
     1861 cggacatcat tctcgtcggc gaattgcgcg acctggaaac catcgaactc gccatcactg
     1921 ccgcgagcac gggtcacctg gtctttggta cgttgcacac gcaaagcgcc ggcaagacgg
     1981 ttgaccgtat cattgacgtt ttcccgtccg agcagcagaa caaaatccgc gccacgcttt
     2041 ccgaagcact caagggagtg gtcgcccaga atcttttcaa gcgcgttgat aaaaaaggcc
     2101 gcgtcgccgc cctcgaaatc ctggtgttca ccaccgccat cgcaaacctt gtgcgtgaag
     2161 gcaagacgca ccaaatcccc ggcatgatcc aagtcggcaa aaaacacggc aaccagccgc
     2221 tcgacgacgc catcatggag catctgcgca tgaagcgcat ttcaccggaa gaggcctacg
     2281 acaagtgcct ggacaagaaa aaatttcgga cctttctggc caatcctcct gacgaggacg
     2341 agacctgagc cgattaaatt atgcgccgtc ctgaactgga cagtatctta tcgacgatgc
     2401 tcgatgccgc gccggaggtc tccgaccttc tgttcacggt ggacaagccg ttgcaagttg
     2461 aagccgcagg cgaactcacc ccggtcgaaa gcgaaccgcc gctggtccgc ctcactccct
     2521 accagaccga ggtcatcgcc ctcaatctca tcggcgaaaa catctggcag atcaaggacc
     2581 tgctccgcac gggctcttgc gacgccgcct acaccgtcat ggacaaggcg cgttttcgta
     2641 tcaacatttt ttcgcaacgc ggcaattact ccatcgtcct gcgcaaactg aacaccaaga
     2701 tcccgacgct tgaaagcctc aagctgccgg aaatcatcaa tcaaattccg cgtgaaaaaa
     2761 ccggccttgt ccttgtcacc ggcgcgaccg gttccggaaa aagcaccacg ctggccgcag
     2821 tgttgagcga attcaaccgc acgaaggcca tccacgtcat cacgctggaa gatccgatcg
     2881 agtttgtgca tccgcatgac aaggccactt tcaatcagcg cgaattgggc actgattacg
     2941 acagttttgc caacggcttg cgcgcggctc ttcgtcaggc gcccaaagtg attctcgtcg
     3001 gcgaaatgcg cgacaaggaa acggtcaaga tcgcgttgag cgccgccgag acgggccatc
     3061 tcgtgctcag cacgctgcac accgtggacg ccggacagac cgtcaatcgt attctgggca
     3121 tgttcgaaac tgaagagcag gagcagatgc gcctgcgcct cgccgacacg ttgcgctggg
     3181 tcgtcagcca gagattggtc cccaaaatcg gcggcggccg ctgggcgctg ttggaaatca
     3241 tgggttccaa tcttcgcacc aaggaaacca tcgtgcaggg cgaaagcgag ggcaaaacct
     3301 tttacgaaat catcgagtcg agccagtcat ttggctggcg cacgttcgat tcctcggcag
     3361 tggaagccta tgatcaaggc gtcatcaccg aggagagcgc cattctttat tgctccaaac
     3421 gcggccctgt gtcgcgcgcc attgacaatt tgaagaaatc tcgcggcgaa agcacgactc
     3481 aactgaatgg cttgcgcatg aaggcgccag tcaataccgg caacggcagc aaaccctcgc
     3541 ctcccatccc tgcaaccctc aaactcaaat aaccatgtcc gacgaatttg attttatcgg
     3601 cgcagcggac aagcccgccc tgctggcctt ctcgacaccc gactggcttg agatggcgcg
     3661 cggcgcgctc cttgagcttg gctacaagat ccacacggcg gcgacgcaca gcgatttcct
     3721 aatccgcttc acccaggtcc gctatcaagt cgtcatcatc gaggaacttt tcgcggccaa
     3781 cacccttgag gaaaacctca cgctgatcaa tgtgcaaaac atgccgatga accagcgccg
     3841 tcatgccgcc gtcatcctgc ttggaaactc ttttcagacc ttcacaccaa tgcaggcgtt
     3901 ccaattcagc gtgagcgccg tcatcaacgc ctccgagatt ttccttctca agcagctcgt
     3961 cgaaaagtct gtcgcggacc ataccttttt cttccacagc ttccgagaag cccaatccag
     4021 tcttttccgc caggcgacgg cgtgatggtt tcggtcaagt cgtcaaacgg ttgcacagtt
     4081 accaaaataa tgccgttttg ttcaagcgtg tgcatctctg ccgatggtca aatggtcatc
     4141 ggtgtccatg cttcctttta cgcattttcc tgctctgctc aattgcgccc tgcgcgaaag
     4201 cttctaaatc aaactcatga acttttccgc atacggaaca gtgataatgc ccggcgcctt
     4261 ttcccgcaat aacgatgtcg aaatgctttc cgcatgcaga acagtaaatc atcattgaga
     4321 caggaaagtt ccatttgaaa ggcgtctgct gcggcgacct ggcgtccgaa ctcacccact
     4381 gtgcatactc aatcaatttg gcgcactttt catcttcagt tggctcagaa gcggccgaag
     4441 cggaaagatg atgcactatt ccaagcgcaa cgaaaacctc gagaaaatat ccgcagcccg
     4501 ctgtgactgc ttcttcagga acccgtccat catccagtgg acgcgcgacg agagctttcg
     4561 tggaagcgtg ccaggggtct gcggcataga tcgtcaaacc gcgatcaagt tgctcaagtt
     4621 ccgggattat ttctttgagt gtcatgatta tgcctggccc aaataacttc agcgggccgc
     4681 ttcggcacgc gcccacgaat ccgccgctaa aatttgttcc aacgtcggat gagatgtcac
     4741 ctggtggcgc tccatggtgc ggcgaacggt atcagtgatc tgtgtgaatg tgatgcgacg
     4801 gttgacgaac gcttcgacgg caacttcgtt ggcggcattg agaactgcgg gaagtgttcc
     4861 accaatttca ccagcctgtc gagccagatt cagagaagga aagcggtcca cgtcgggttc
     4921 ttcaaacgtc agcgtgccca gttttgcgag attggtttgg acgcgatcac tgcgggcgcg
     4981 ttcgggatag gtcaacgcgt attggatggg caggcacatg tcgggcgtcg aaagctgggc
     5041 gatgatggag ccgtccacga attccaccat cgaatgaaca acgctttgcg gatgcacaac
     5101 caccttgacg cggcccatct cgatgtcgaa gagccagcgc gcttcaatca tttcaaggcc
     5161 cttgttgaaa agcgtcgcgg aatcaatcgt gatcttgcgg cccatgaccc atgaaggatg
     5221 tttcaacgcg cgctcgacgg tgatcgcggc aaattcctct ttcggtgtcg tacggaacgg
     5281 tccgccggac gcagtgagcc aaagattgcg aacggaatgg ccgggcttct cctccaagca
     5341 ttgaaaaatc gcggaatgct cgctgtccac ggccaggacg cggacgccat gtttgcgcgc
     5401 ttcgctcatg acaatctcgc cagccatcac gagaatttcc tttgaagcga ccgcaatgtc
     5461 cttgcccgca cggatcgccg ccagcgccgg ttgcaaaccg gctgtgccga caatcgcgat
     5521 caggacaatg tccgccgacg gcatcgtcgc gagtttgatg agtccatccg tgccggaata
     5581 gacttctgtt gaagtgccaa gagcgtttct ggcttccttc gctttggcgg gatcagtgat
     5641 ggaaatggcg gttggttgat gtttgcgggt ctgttcgaga agcagttcca aattgttgcc
     5701 tgcgccaagt cccacgagcc gaatcttgtc gggcaaatca tcggcgacct tcaccgtgct
     5761 ggtgccgatg gaacccgtgc tgcctaggag aacgacattt ttcattttca atttacgata
     5821 tacgatttac gcgcgcctgg aagaacgggc ggttttggct gatgaaacga ttatggccag
     5881 aatttcatgc ccctcttgca tcaggcttcg aagacgggat tctctgataa gactattatc
     5941 aaccaaaatt tccatccaaa acagagactc atcgcattct tcttcgacca cgcggagctt
     6001 gttgataaaa tccgcggttg atttggcgcg gcagacagca cggtaatttg ctccgactga
     6061 cgttccactg cggagaagct ggcctccgag agtccgcgcc agccaattgt tcggcagggc
     6121 ctggcacaaa cgaacgacac ggttggcata agttttcgtc cttagcatca tttcttcacg
     6181 tgtcatagtt ttcctattcg cgcgtcaatc gtatatcgta aatcgtaaat tcaaggatgc
     6241 attatcacgt gtcgcaaata gatatacatc agcggcgcat taaacaagag gctgtcgagc
     6301 aaatccaaaa tgcctccgat gccgggaaaa agatggccgg aatccttgac tcccgcttcg
     6361 cgtttgaaaa gcgattcgat caaatcgccg atgaccgccg agatgctcaa caccacgccg
     6421 agtaccacgg catgtttcca gttcatcgcc ggcatttcat cgtgcgcaaa atggacaaag
     6481 agcacgcttg cgacggtcga aacgacgatt gcgcccccaa aaccttccca ggtcttgccg
     6541 gggctgatgc ggggaatcat cttgtgtttg ccgatgagcg aaccgaccga gtacgcgccc
     6601 atgtcgctga attttgtgat gagcaggaaa taaagcaggt aaaaatggcc gtttataccc
     6661 gggaaaaaat tgattttttg gatgaaattc agcagccacg ggacatacat caggccaaaa
     6721 agcgtcgttg agatggcgag aatccctgcg gtgttgcttt tggaaacgaa ctggcgcacg
     6781 caaaggccga gcacgaagag gatgagaaag ctggtctcga aatcgttgac gcgcgacggt
     6841 aaataattgt gcgttccaac aaccccggtc aggtttaaaa acgtgccaat catcagcaaa
     6901 gacccgccca tcaatcccga atatttgaaa cagacgaggt cgcgcttttc cacaagcccg
     6961 taaaattccc acaatccgga acacgcaagc agcactatca ccaccagaaa aaccaggtca
     7021 gagatcagtt tgttgccgga aaaaagcccg gccaaaatga tcgtccagag catcacgaag
     7081 ctgaacaacc gccggaaaaa gatttgccgc tttgaaagtg ctggcgcaac ggcaattgtt
     7141 tcgttcggca tacgtttgaa cttaccgatt cacagcgcgg tgtcgaggcg attgcttatc
     7201 cctactggta tttcttaacg tattcgccgg taagtcttac aagttctgca agattaacga
     7261 tctcgccttt ttcaacccgt ttggaaatca tggtaagcgc attgatttgc catgtggttt
     7321 ctttgaagcg caacgtcggg tccgtcccgt attcgtcagt gaccatgacc catcgcaaag
     7381 gaaattcgct cgcaagcaca tcgccgaatg taatcccaag acattgcaat tcgtaagttt
     7441 ggcttttttt gaaaactttg tcgtcgagta tgcgttggag gatcggcaaa tccgacttcg
     7501 tctttgtcag ggcaggtgcg ccatatttct gcattgcgat aacggcgatt aaatgtcgct
     7561 gcttttccaa ccgagcggca tcctccttcg aaagttctgc aaatttcgga ctcgaagtct
     7621 gagacatatt ttttggattt tgggcgattg aaagatcaac aaacaacatc gttacaagaa
     7681 cgaacaacag agtttttttc atattccccc aaaccgccta tgtcgctggg cgtattcttc
     7741 caacgcggtg atgaattgcg gcttgcgaaa atcaggccag agcgtctgcg tcacgaccag
     7801 ttcggcatag gagatttgcc agagcaaaaa attgctgacg cgcatttccc cactggtgcg
     7861 gatcaacaca tcgggatcag gccatttgcg cgtgtaaaga tggtcggcg
//
LOCUS       DASZMM010000065         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3320732, whole genome shotgun sequence.
ACCESSION   DASZMM010000065 DASZMM010000000
VERSION     DASZMM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3320732"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="tyrS"
                     /locus_tag="VG754_01685"
     CDS             complement(<1..1149)
                     /gene="tyrS"
                     /locus_tag="VG754_01685"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWF17946.1"
                     /translation="MNIYDELEWRGLVADCTDKEELLKRLNTAPITLYCGFDPTADSL
                     HVGNLVPLLALRRFQLMGHQPIAVAGGATGSIGDPSGKSAERQLLTREVLDANIASVK
                     EQLRRLLDFETKTNPARLVDNATWTAPVSYLDFLRDIGKHFSVNVMVAKESVRARMED
                     RESGISYTEFSYMLLQAFDFYYLRKEYNCELQIGGSDQWGNITAGIDLARKKIGAHVF
                     GLTVPLILNADGSKFGKSVSGAIWVNPAKTSVYRFYQFWIRTDDRDVVRYLKFFTFLT
                     REEIAVLEKQHQENPGARDAHKALARAMTDLIHGAAATAEAIRASEILFGGGLEGISE
                     STFNDIVGEVPSKEIEKSKLDAAGMPLVELLVHSGLSPSKGQARKDVEG"
     gene            complement(1211..2173)
                     /gene="egtD"
                     /locus_tag="VG754_01690"
     CDS             complement(1211..2173)
                     /gene="egtD"
                     /locus_tag="VG754_01690"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWF17947.1"
                     /translation="MIQEPVLAAGELTVEDFRRDVLAGLSQPEKILRCKYLYDERGAR
                     LFEEICELEEYYPTRTELAILKWHIKEIAALPGPNCRLVELGSGSGLKTRLLLEHLDH
                     PAAYVPVDISRSQLFECAERVARDHPRADVLPICADYTNHFELPEPPAHANKTIIFFP
                     GSTIGNFEPAEAADFLGRIAHFCGGDGGILLGVDLKKTTSMLKRAYNDSRGVTAEFNL
                     NLLDRINRELDGDFDRSQFWHHALYNETAGRIEMHLVSARRQTVAVCGHEFHFAAGES
                     VVTEHSYKYTPGEFRRLAAVANLEVARCWTDDRRWFSVQYLVPR"
     gene            complement(2204..>2821)
                     /locus_tag="VG754_01695"
     CDS             complement(2204..>2821)
                     /locus_tag="VG754_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWF17948.1"
                     /translation="RPVTNGEYLAFIEDGGYQRSELWLSLGWNTINERGWTAPLYWEK
                     SDDSVWWQMTLAGMRKVQSYEPVCALSYFEADAFARWAKARLPTESEWEVAVNGLPIE
                     GNFAESGFFHPTPPDESPFSDELQQMFGDVWQWTQSAYSPYPGYASAKGALGEYNGKF
                     MCNQYVLRGASCATPESHARRTYRNFFPPDARWQFSGIRLARDLV"
BASE COUNT          601 a          818 c          808 g          594 t
ORIGIN      
        1 gccttcgacg tccttgcggg cctgcccttt ggaaggcgac aaccccgaat gaaccagcaa
       61 ctccaccaac ggcattcccg ctgcatccag tttgcttttc tcaatctcct tggacggcac
      121 ttcgccgacg atgtcgttga aggtgctctc ggaaattcct tccaggccgc cgccgaacag
      181 aatttcactg gcgcgaatgg cttcggcggt cgcggctgcg ccgtggatca aatcggtcat
      241 ggccctggcg agcgccttgt gggcgtcgcg cgcaccggga ttttcctggt gctgtttttc
      301 cagcacggcg atttcttcgc gggtgagaaa cgtgaaaaat ttcaggtaac ggacgacgtc
      361 gcggtcatcg gtgcgtatcc aaaactggta aaagcgatag acgctggtct tggcaggatt
      421 gacccagatg gcgccggaga cgcttttgcc gaacttggac ccgtcggcat tcagaatgag
      481 cggcacggtg agaccaaaga cgtgcgcgcc gatttttttc cgcgcgaggt cgatgcccgc
      541 ggtgatgttg ccccattgat cgctgccgcc gatttgcagc tcacagttgt attccttgcg
      601 cagataataa aagtcgaatg cctgcaacaa catgtagctg aattcggtgt agctgatgcc
      661 cgattcgcgg tcttccatgc gggcgcggac gctttccttg gcaaccatca cgttcacgga
      721 aaaatgtttg ccgatgtcgc gcaaaaaatc gagataactg accggcgcgg tccatgtggc
      781 gttgtcaacg agccgcgcgg gattggtctt ggtctcgaaa tcgagcaggc ggcggagttg
      841 ttccttgacg ctcgcgatgt tggcatcgag aacttcgcgc gtgagcaact ggcgttcggc
      901 gctcttgccg ctgggatcgc cgatggaacc ggtcgcgccg ccggcgacgg cgatcggctg
      961 atgtcccatc agttgaaagc ggcgcaatgc gagcaacgga accaagttgc cgacgtgcag
     1021 gctgtccgcc gtgggatcga acccgcaata aagcgtgatg ggcgcggtat tcaaccgctt
     1081 gagcaattcc tctttgtccg tgcaatcagc cacgaggccg cgccattcca gttcgtcgta
     1141 aatgttcatc cgcgaacgga ttgattagca cagcgagcga aacgtcgcca tgaaaaagaa
     1201 acatgccaaa ttaacgcggc accaaatatt gcacgctgaa ccagcggcgg tcgtcggtcc
     1261 agcaacgggc gacttcaagg ttggcgacag ccgcgaggcg gcggaattcg ccgggcgtgt
     1321 atttgtagga atgttccgtg acgacggatt ccccggcggc gaaatgaaat tcatgtccgc
     1381 agaccgcaac cgtttgccgg cgcgcgctga cgagatgcat ttcaatcctg ccggcggttt
     1441 cgttgtagag agcgtgatgc cagaattgag aacggtcaaa atcgccgtcg agttcgcggt
     1501 tgatgcggtc gagcaggttc aagttgaatt ccgccgtgac gccgcgcgaa tcgttgtaag
     1561 cgcgtttgag catcgaggtg gttttcttca ggtccacgcc cagcaagatg ccgccgtcac
     1621 cgccgcaaaa atgcgcgatg cgcccgagaa aatccgcggc ttcggccggc tcgaaattgc
     1681 cgatggtcga accgggaaaa aaaatgattg ttttgtttgc gtgcgcgggc ggctccggca
     1741 attcaaaatg atttgtgtaa tcggcgcaaa tcggcagcac gtcggcccgg ggatgatcac
     1801 gagcgacccg ttcggcgcat tcgaagagtt gggaacgcga gatgtccacc ggcacatacg
     1861 ccgccggatg gtcgagatgc tccaggagca aacgtgtctt gagcccgctg ccgctgccca
     1921 attccacaag acggcaattt ggccccggca acgccgcgat ctccttgatg tgccatttca
     1981 gaatcgccag ttccgtgcgt gtcggataat attcctcaag ctcgcaaatc tcctcgaaca
     2041 gccgcgcgcc gcgttcgtcg taaaggtatt tgcatcgcaa tattttttcg ggttgcgaaa
     2101 gtcccgccag cacatcgcgc cgaaaatcct ctacggtcaa ttctcccgcg gccaaaaccg
     2161 gttcctgaat catgactccc tttctagaat tttgaaattg cgatcaaacc aaatcccgcg
     2221 ccaagcggat gccgctgaac tgccagcggg catcgggcgg aaagaaattg cgatacgttc
     2281 gccgcgcatg cgattccggc gtcgcgcacg aagcgccgcg cagcacgtat tgattgcaca
     2341 tgaacttgcc gttgtattcg cccaaagcgc ctttcgcgct cgcataaccg ggataaggcg
     2401 agtaggcact ttgcgtccat tgccagacat cgccgaacat ctgctgcaac tcgtcggaaa
     2461 atggcgattc gtccggcggc gtgggatgaa aaaatccgct ctcggcaaaa ttgccttcaa
     2521 tcggcaaacc attgacggcg acttcccatt ccgactcggt gggcaaccgc gccttcgccc
     2581 aacgcgcgaa tgcgtccgcc tcgaaataac tgagcgcgca aaccggctcg tacgattgca
     2641 ccttgcgcat tcctgccagc gtcatctgcc accagacgct gtcatcggat ttttcccagt
     2701 acaacggcgc ggtccagccg cgttcattga tagtattcca tccgagcgac agccaaagtt
     2761 ccgaacgctg ataaccgccg tcctcgatga aagcgagata ttcgccattg gtaaccggcc
     2821 g
//
LOCUS       DASZMM010000066         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4965468, whole genome shotgun sequence.
ACCESSION   DASZMM010000066 DASZMM010000000
VERSION     DASZMM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4965468"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="VG754_01700"
     CDS             <1..1702
                     /locus_tag="VG754_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWF17949.1"
                     /translation="DYKTAGVVAFFMLMGELIETRTAEGARQSIESLIKLTPTKARRI
                     TAQGEEEVAAKDLAINDVIRVRPGDNVAADGVILNGQGSFNQATITGESLPVDKKPGD
                     EVFAGTQNLTGVLEIRVSRAGQDTTLGKVRELILAAEKTKLPIMRIIDQYMGFYTPLV
                     LVIGALVWAFTRDLNRVISVLVVSCPCAFILATPTAMVAALSAAARLGILIKNVSDIE
                     LAARINAFVFDKTGTLTTGKLAVSRLAPIGEHQPAELLRVAASAEKYSNHPTAKALAQ
                     LADEAGVPLAEPKHFAETAGRGIKAEVEGKAVLVGRAQWLKDNGVKDDFMKAVDLSEA
                     EGFSLLFIAQAGHCIGWIGLQDQTREEARESLAELKACGVRRIAMISGDRQPVASRVA
                     KEIGCEEVVGECLPQNKVEFVRAIKLKGYRVAVVGDGVNDAPALAAGDIGIAMGAAGS
                     EVAIHSATIALMNNDLRRLPFLVKLSRSTRAVINQNFLFGVLFIITGLSLAAFGVVGP
                     IVAAIMHNLGSLMVIFNSARLVRKGEELEHYQPVPVEPPARSSGQKPKPAPEMAPTMA
                     "
     gene            1716..1901
                     /locus_tag="VG754_01705"
     CDS             1716..1901
                     /locus_tag="VG754_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002752119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWF17950.1"
                     /translation="MKIRDILRIIEEDGWRQVRQRGSHRQFKHPAKTGLVTVAGHPRD
                     DVAPGTLNSILKQAGLK"
     gene            1898..2113
                     /locus_tag="VG754_01710"
     CDS             1898..2113
                     /locus_tag="VG754_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF17951.1"
                     /translation="MKKYLMVIEETGTGFSVYSPDLPGCIATGATREEAERQMRDAMQ
                     FHIEGLKLEGLDVPEPHSYPAFCEIAA"
     gene            2215..3441
                     /locus_tag="VG754_01715"
     CDS             2215..3441
                     /locus_tag="VG754_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF17952.1"
                     /translation="MTTAILPTPHSAPRTPHSHEVVIAVRGLTKVFKDFWGRPKARAV
                     DNVDFEVRRGEVFGLLGPNGSGKSTTVKMLLGLLYPTKGHIEVFGHSPRHVATKSRIG
                     YLPEESYLYRYLNSHETLDFFGNLFRLPAGERQKRSEQLLEMVGLSQTRTRAVGEFSK
                     GMQRRIGLAQALINDPDLVILDEPTAGLDPIGCREVKDLILALARRGKTVILSSHLLS
                     DVEDVCDRVVIYYGGKIQAMGTLKELLATPDVVRITTPVLQRETLERALEIIRKDIDG
                     DKIRIDNPTQNLESYFLDVVQKARQAAEATSGATSGHRVAAYLRGDAEAKPTTDRVLE
                     RLTAPQPSAAPAPVAAPQPADTVDRKKLDALTRGHEAETPAPTPKTAAQSAPADPVDL
                     KKANEKLSSLLGKPKD"
BASE COUNT          846 a         1153 c         1137 g          768 t
ORIGIN      
        1 cgattacaag accgccggtg tggtggcgtt tttcatgttg atgggcgagt tgatcgagac
       61 ccgcaccgcg gaaggcgcgc gtcagtccat cgaatcgctc atcaagctca caccgaccaa
      121 ggcgcggcgc atcaccgcgc aaggcgagga agaagtggcg gcgaaagacc ttgccatcaa
      181 cgatgtcatc cgtgtccgtc ccggcgacaa tgtggctgcg gacggcgtca tccttaacgg
      241 ccagggttcc ttcaaccagg cgaccatcac cggcgaatcg ttgccggtgg acaagaagcc
      301 cggggacgaa gttttcgccg gcacgcagaa tttaacgggc gtccttgaga tccgcgtcag
      361 ccgagccggg caggacacca cgttgggcaa ggtgcgcgaa ttgattttgg cagccgagaa
      421 aaccaagctg cccatcatgc gcatcatcga ccaatacatg ggtttttaca cgccgctggt
      481 gctggtcatc ggcgcgttgg tctgggcatt cacgcgcgac ttgaaccgcg tcatctccgt
      541 gctcgtcgtt tcctgtccct gcgcgttcat ccttgcgacg cccacggcga tggtcgccgc
      601 gctttccgcc gccgcgcgtt tgggaatcct tatcaagaac gtcagcgaca tcgaactcgc
      661 cgcgcgcatc aatgcgttcg tattcgacaa gaccggcacg cttaccaccg gcaaactggc
      721 tgtcagccgg ctcgccccga ttggcgaaca tcaaccggcg gaattgctgc gggtcgccgc
      781 ttccgctgaa aaatacagca accatcccac cgccaaggcg cttgcgcagc ttgcggatga
      841 agcgggtgtg ccgctggccg agccaaaaca tttcgccgag accgccggac gcggcatcaa
      901 ggctgaagtt gaaggcaagg ctgtcctcgt gggccgcgcg caatggctca aggacaacgg
      961 agtgaaggat gatttcatga aggcggtgga tttgagcgag gctgaagggt tcagtctgct
     1021 gtttatcgcg caggccgggc attgcatcgg ctggatcggt ttgcaggacc agacccgcga
     1081 ggaagcgcgt gaatcgctgg ctgaactcaa ggcttgcggc gtgcggcgca tcgcgatgat
     1141 ttccggcgac cgccagccgg tggcgtcgcg tgtggcgaag gaaatcggct gcgaagaagt
     1201 cgtgggcgaa tgcctgccgc agaacaaggt tgaatttgtg cgcgccatca agctcaaggg
     1261 ctatcgcgtg gcggtggtcg gcgacggcgt gaacgacgcc ccggcgcttg ctgcgggaga
     1321 catcggcatc gcgatgggcg cggcaggcag cgaagtggcg attcacagcg cgacgattgc
     1381 gctcatgaac aacgacctgc gccggttgcc gtttttggtc aagctttcgc gcagcacccg
     1441 cgcggtcatc aaccagaatt tcctgttcgg cgtgctgttc atcatcaccg gtctttcgct
     1501 cgcggcgttc ggagttgtgg ggccgatcgt ggcggccatc atgcacaatc ttgggtcgtt
     1561 gatggtcatt ttcaacagcg cccggctggt gcgcaagggc gaggaactgg agcattacca
     1621 acccgtgccc gttgaaccgc ccgcgcgttc gagcggtcaa aaacccaagc ccgcgcccga
     1681 aatggcgccg acgatggcgt gaagttttcg ggctgatgaa aatccgcgat attttgcgga
     1741 tcattgaaga ggatggctgg cgtcaggtgc ggcagcgtgg cagccatcgt caattcaagc
     1801 atccggccaa gacaggcttg gtgacggtgg caggacatcc gcgcgacgat gttgcgccgg
     1861 gtacattgaa cagcattttg aaacaggcag gacttaaatg aaaaaatatt taatggtgat
     1921 agaagaaacg gggaccggct tctctgttta ttcacccgat ttgccgggct gcatcgcgac
     1981 cggcgcaacc cgcgaagaag ccgaacgcca aatgcgcgac gcgatgcaat ttcacatcga
     2041 ggggcttaag ctcgaaggtc ttgatgtgcc cgaaccgcac agttatcccg cgttctgtga
     2101 aattgctgct taaatatttg ccggtcagaa actgccgagg atgaatgaca aaaatgtctt
     2161 gcgaatgaac tatctctccg agttggataa gctcaccgcc aacagcacta aagcatgacc
     2221 accgcgatcc tcccaactcc gcactccgca ccccgcactc cgcactccca cgaagtggtg
     2281 atcgccgtgc gcggattgac caaggtgttc aaggacttct ggggccggcc aaaggcgcgc
     2341 gcggtggaca acgtggattt tgaagtgcgc cgcggcgagg tgttcggttt gcttggcccg
     2401 aacggctccg gcaaatccac caccgtcaag atgctccttg gcctgttgta tccgaccaag
     2461 ggccacatcg aagtcttcgg ccattcgccg cgtcacgtcg ccaccaagtc gcgcatcggc
     2521 tatctccccg aggaatctta tctttatcgc tatcttaatt cgcacgagac gcttgatttt
     2581 ttcggcaacc tttttcgcct gcccgccggc gaacggcaga agcgctccga gcaattgctc
     2641 gaaatggtcg gcttgagcca gacacgcacg cgcgccgtcg gcgaattttc caagggaatg
     2701 cagcgccgca tcggccttgc ccaggcgctc atcaacgacc ccgacttggt cattctcgat
     2761 gaaccgaccg ccggcctcga ccccatcggc tgccgcgaag tgaaggacct catccttgcg
     2821 ctcgcgcggc gcggaaagac ggtcatcctg agcagccact tgctttccga tgtcgaggac
     2881 gtttgcgacc gcgtggtgat ttattacggc ggcaaaattc aggccatggg cacgctcaaa
     2941 gaattgctcg cgacacccga tgttgtccgc atcacaaccc cggtgctcca gcgtgaaacg
     3001 ctcgaacgcg cccttgaaat catccgcaag gacattgacg gtgacaaaat ccggatcgat
     3061 aacccgacgc aaaatctgga gagctacttt ctcgacgtgg tccaaaaagc gcggcaagca
     3121 gccgaagcaa cttctggcgc gacctccggg catcgcgttg cggcgtactt gcgcggcgat
     3181 gccgaagcca aaccaaccac cgaccgcgtt ttggaacgcc tcaccgctcc gcaaccttcc
     3241 gccgcgcccg cgcctgtcgc tgcgccgcaa ccagccgata ccgttgatcg caagaaattg
     3301 gacgcgttga cgcgcggtca cgaagccgaa acgcctgctc caacgccaaa aacagccgca
     3361 caatccgctc ccgcagaccc ggtggattta aagaaagcga acgagaaact ttcgtcgctg
     3421 ttgggcaagc caaaggatta atttttaagc cacggatgaa acacggatga aacacggaat
     3481 taaaaaaaca gggcttggtc cacggctgac acgaacattg tttctggaac aatggccatt
     3541 gctgtccccc tcgccatctt cgatggcaca acctcctttc ggcagaacgt gctcgtattg
     3601 gaacagagac aggaaagatt ttctccctct ccccaaggga gagggatggg gtgaggggaa
     3661 aggaaatatc cgatttcaca gatggagcaa accgggtaaa ttatcgtgtt ccattcagag
     3721 aaccccgcaa accggtgttc ttccatccat tttcgcgaaa atagcataat gcaacgactt
     3781 ttcgccatca tcacgctgac ttggaaggcc gcttttcgtt accggctttt ttgggtcatc
     3841 acgtcgctgt tgctggcggc ggtgatattc ctgccgctcc tgatcaaaga tgacgggacc
     3901 gcgg
//
LOCUS       DASZMM010000067         5033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3866767, whole genome shotgun sequence.
ACCESSION   DASZMM010000067 DASZMM010000000
VERSION     DASZMM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3866767"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="VG754_01720"
     CDS             <1..1664
                     /locus_tag="VG754_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HWF17953.1"
                     /translation="WCVYPNFDEIPEYTGYLKPKNFEIFRDFLNANGMGNLSHQYLLA
                     SGKLQTLCYKEDIESALRTPGMGGFELLDLHDFPGQGTALVGVLDPFWHDKGYVTAKE
                     YSRFCNRTVPLARLPKRVFTTDETLTGRLELAHFGPGPIEGAVTHWRFMNDKQVLAEG
                     KLPAKDIPVGNGISLGDVAIDLKNVPAPAHCKLVVSVADAENDWDIWVYPTNTVSPAP
                     TGIMVTSRFDATARARLQVGWKVLLTIPAKQVRNYDTDPVKVGFSSIFWNTAWTARQA
                     PTTLGILCDPKNPALSEFPTDFYSDWQWWYLIHRAGALRLDLLPSGAEPIVRVIDDWV
                     TAHPLALVTEAKVGTGKMVICGFDLTEGADDPVSRQMLRSLLDYMDSPKFAPRTECTL
                     DQIQSLQIQSHVVPAAPKASSGIAHIKADSEQEGYEVKNAIDGNPQTFWHSSWAEGSP
                     GFPHELTIELSDAKMVAGITALPRQDGNQNGWIKDYLLYTSLDGENWGDPVARGTFTK
                     TRRLKTVKFSAPTRAKFIKLVAESGYADGSWASLAEFNVIDANEN"
     gene            2064..3485
                     /locus_tag="VG754_01725"
     CDS             2064..3485
                     /locus_tag="VG754_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWF17954.1"
                     /translation="MITAIKSDTREIKADYKLGYIWLISVVAALGGLLFGWDWVVIGG
                     AKPFFQRYFDLQDPSLIGWANSCALIGCLIGALVAGALSDRFGRKRLLILSALLFAVT
                     SIGNALAPTFSIFIAWRILGGVAIGLASNLSPMYIAEVAPAAMRGKLVSINQLTIVVG
                     ILLAQVINWSLVRNLPAGATDEFIRNSWFGQSGWRWMFGLTAAPSLLFFMGMFFVPES
                     PRWLAKNGKSDAARNILKSIGGESYSQAALGEINSTLEGEEIQHVRFQDLLDPKMMRV
                     LGLGIVLAVFQQWCGINVIFNYAEDIFREAGYDISTVLQNIAWTGSVNLAFTFVALGT
                     VDRGGRRPLMLFGSAALALIYLALGLCYYNGVKGLPTLTLVLAAIACYSMSLAPVTWV
                     VISEIFPNRIRGAAMSVAVSSLWIACFLLTFTFPFLNKKLGAAGTFWLYAVICVLGFF
                     FIRYKLPETKGKTLEEIERALVD"
     gene            3469..>5033
                     /locus_tag="VG754_01730"
     CDS             3469..>5033
                     /locus_tag="VG754_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosylceramidase"
                     /protein_id="HWF17955.1"
                     /translation="MRWWIKRTWLLVFGAMAVHRAFALDITLDPHQPGRTFDGIGAVS
                     AGASSRLLIDYPEPQRSQILDYLFKPNYGAALHHLKVEIGGGVNSTDGTEPTHMRSLT
                     DQNYSRGYEWWLMSNAVSRNTNIILDCLAWGAPGWIGNGNYYSQDMCNYIVNFLKGAQ
                     THYGLNFTYTGTHNEATVNTTWIKLLRTTLNANGLQNVQLVAADEWGGTWNIVTNTSY
                     GLLVDPALSNAVARVGAHYPGQTSPAAAQTCGKPLWSSEDGIGGSTWSAARNLGKIFN
                     LNYVTGKMTATEIWSPITSYYDILAAANSGLMRANTPWSGNYMVAPAIWAAAHTTQFA
                     YPGWTYLEGGASALLPSGGSVVTLESTNGSDYSIIVETFDATAAQTITFHLTNGLSQA
                     QVNVWETTQTSQFIKVSQLTPSNGSYTFTFQPECLYTLTTTTGQTKGSATPPAATPFP
                     LPYKDSFENYAVAQTPKYFSDQAGTFETFTRADGKGQCLRQVLTRIGIRWTSEWQPYT
                     LLGDANWQDYDVAS"
BASE COUNT         1114 a         1362 c         1410 g         1147 t
ORIGIN      
        1 aatggtgcgt ctatcccaat ttcgatgaaa ttccggaata caccggttat ctcaagccca
       61 aaaatttcga gattttccgc gattttctga acgccaacgg aatgggaaat ctgtcgcatc
      121 aatacctgct cgcgtcgggc aaattgcaaa cgctctgcta caaggaagac attgaatcgg
      181 ctttgcgcac gccggggatg ggcgggttcg aattgctcga cctgcacgat tttcccgggc
      241 agggcaccgc ccttgtgggc gtgctcgatc cgttttggca cgacaaaggt tatgtgaccg
      301 caaaggaata cagccgtttc tgcaatcgca ccgtgccgct ggcgcgtttg cccaagcggg
      361 tgttcaccac cgatgaaaca ttgaccggca ggctcgaact ggcccacttc ggccccggac
      421 cgatcgaagg cgcggtgaca cattggagat tcatgaacga caaacaggtt ttggctgaag
      481 gcaaactgcc tgcaaaagat attccggtcg gcaatggaat ttcgctcggc gatgtcgcga
      541 ttgatttgaa gaacgttccc gcgcccgcgc attgcaagct ggtcgtgtcg gtggctgacg
      601 cggaaaatga ttgggacatc tgggtttacc cgacgaacac tgtctcccct gctccaacag
      661 gcatcatggt gacatcacgg ttcgatgcca ccgcgcgcgc gcggttgcag gtcggctgga
      721 aagtgctcct gacaattccc gccaagcaag tgcgcaatta cgataccgac ccggtgaagg
      781 tgggattctc cagcatcttt tggaacacgg cgtggacagc acgacaagcg ccgacgacgc
      841 tgggcattct ttgcgaccca aaaaatccgg ccctgtcgga atttccgacg gatttttaca
      901 gtgactggca atggtggtat ctcatccatc gcgcgggagc gttgcgcctc gacctgttgc
      961 caagtggcgc cgaaccaatt gttcgtgtca ttgacgactg ggtgacggcg catcccctgg
     1021 ctttggtcac cgaggcaaaa gttggaacgg gcaagatggt catctgcgga ttcgatttga
     1081 ctgaaggcgc ggacgatccc gtctcacgcc agatgttgcg aagtttgttg gactacatgg
     1141 attctccgaa atttgcgccg cgaacggaat gcaccctcga tcaaatccaa tcgcttcaaa
     1201 tccaatcgca tgtcgttcca gcggcgccaa aagcctccag cggcatcgct catatcaagg
     1261 ccgattccga acaggaaggt tatgaagtga agaatgccat cgacggcaat ccgcaaactt
     1321 tttggcatag ttcctgggct gaaggctctc cgggatttcc ccatgaactg acgattgaat
     1381 tgagcgatgc aaaaatggtc gccggaatca ccgccttgcc gcgacaggac ggcaatcaaa
     1441 acggatggat caaggattat ctcctttaca ccagcctgga cggtgagaat tggggcgacc
     1501 cggttgcgcg cggcacattt acgaaaacgc ggcgtctcaa gacggtgaaa ttctccgcgc
     1561 caaccagggc aaagttcatc aagctggtcg ccgaaagcgg ttatgcggat ggttcatggg
     1621 cgtcgcttgc agaatttaat gtgattgatg ccaacgaaaa ttaaaggttc ttggccgttt
     1681 tcaatggaat ccgaaatgag aagctggcct gtcatgacga agatgaaaat atcagtggtt
     1741 acgctcgcct tcaccccgac ccctctccca tcgcgccgta acgattcagt tgaaaaggga
     1801 gcgcacgccc ccggcgcgca gtgttcggcg tcccgccgaa cacttcgtaa tactgatttt
     1861 ccatccaacc aaacgggatc caaaaacaga agtgaaagtc ctcgctggga cggctcggac
     1921 agcacgcgag acgcgtgggc tccccttttt catcgacatg atcccgatgg aggtgagggc
     1981 gaacctccaa tacaatcatt gaaaccaacg ttccatcctt tactcattcc accaaaacag
     2041 cgaaggacaa caattaaagc gatatgatta ccgcaatcaa aagcgatacc cgcgaaatta
     2101 aggccgatta caaactcggt tacatctggc tcatttcggt tgtagcggca ctcggcgggt
     2161 tgttgtttgg gtgggattgg gtcgtgatcg gcggggccaa gccgtttttt cagaggtatt
     2221 tcgacctgca agacccgagc ctgattggat gggccaatag ttgcgccttg atcggctgtc
     2281 tgattggcgc gctcgtcgcg ggcgcattga gcgacaggtt tggacgcaag cggttgctca
     2341 tcttgtcggc tttgctattc gcagtcacat cgatcggcaa cgcgctcgcg ccgacatttt
     2401 caatttttat tgcgtggcgc atcttgggcg gcgtggcaat cggtttggca tcgaatcttt
     2461 cgccgatgta catcgctgaa gtcgcgcctg ctgcgatgcg cggcaagctg gtttcaatca
     2521 atcaactgac gattgtcgtc ggcatcctgc tggcgcaggt gattaattgg tcgttggtcc
     2581 ggaatttgcc cgccggtgcg acggatgaat tcatccggaa ctcatggttc gggcaaagtg
     2641 gatggcgctg gatgtttggc ctcaccgccg cgccgtcgtt gttgttcttc atgggaatgt
     2701 ttttcgtgcc ggaaagtccg cgctggcttg caaagaatgg aaaaagcgac gcggcgcgaa
     2761 atattttgaa gtccatcggc ggcgaaagct acagccaggc cgcgctcggc gaaatcaatt
     2821 ccacacttga gggcgaggaa attcagcacg tccgtttcca ggacctgctc gacccaaaaa
     2881 tgatgcgggt gcttggattg ggaattgtgc tggcagtgtt ccagcaatgg tgcggcatca
     2941 acgtgatctt caattatgcg gaggatattt tccgcgaagc cggatatgac atttccacgg
     3001 tgttgcagaa catcgcatgg accggctcag tcaatctcgc attcaccttc gtggcgctcg
     3061 gcacggtgga ccgcgggggg cgacggccct tgatgctttt tggaagcgcg gcgctcgcat
     3121 tgatttattt ggctctggga ctctgctatt acaatggggt caaaggcttg ccgacattga
     3181 cactggtgct ggcggcgatt gcctgctact cgatgtccct tgcgccggtg acgtgggtgg
     3241 tgatttcgga aatttttccc aaccgaattc gcggcgcggc gatgtctgtc gcggtcagtt
     3301 cgctctggat cgcctgcttt ttgttgacgt tcacgtttcc gtttctgaac aagaagctcg
     3361 gggcagccgg aacattctgg ttgtatgcgg tgatttgcgt cctcggtttc ttctttatcc
     3421 gttacaaact gcccgaaacc aagggcaaga ctttggagga aatcgagcgt gcgctggtgg
     3481 attaagcgaa cttggctgtt ggtgtttggc gccatggccg tccatcgggc atttgctctg
     3541 gacatcacgt tggatccgca ccagcccggt cgcacatttg acggcatagg cgcggtgagc
     3601 gcgggcgctt cgtcgcggct gctgattgat tatcccgaac cacaacgcag ccagatcctg
     3661 gattatcttt tcaaaccgaa ttacggagcg gcgttgcatc atctcaaagt cgaaatcggc
     3721 ggaggcgtca attcgaccga cggcaccgaa ccgacgcaca tgcgctcgct caccgatcaa
     3781 aattattcgc gcggatacga atggtggttg atgagcaatg cggtttcgag aaacacgaac
     3841 atcattttgg attgcctcgc ctggggcgcg ccgggctgga ttgggaatgg caattattat
     3901 tcgcaggaca tgtgcaatta catcgtcaat tttttgaaag gcgcgcaaac ccattacggg
     3961 ttgaacttca cctacaccgg gacacacaac gaagcgaccg tcaacacaac atggatcaag
     4021 ttgcttcgca cgaccctcaa cgcgaatgga ttgcaaaatg ttcagctcgt cgccgccgac
     4081 gaatggggcg gcacctggaa cattgtcacg aacacttcct acggcctgct tgtggatccg
     4141 gcgctgtcca atgccgtcgc gcgtgttggc gcgcattatc ccggccagac aagtcccgcc
     4201 gctgcgcaaa cgtgcggaaa gcctctttgg tcgagcgaag acggcatcgg cggcagcacc
     4261 tggtcggcgg cgcgcaattt gggaaagatt ttcaatctca attatgtcac cggcaaaatg
     4321 accgcgacgg aaatttggag tccgatcact tcttactatg acattcttgc cgcggcgaat
     4381 tccggtttga tgcgcgcgaa cacgccatgg tcgggcaatt acatggttgc cccggcgatt
     4441 tgggcggcgg cgcacacgac ccaattcgcc tatccgggct ggacttacct tgagggcggc
     4501 gcatcggctt tgctgccgtc aggcggcagc gttgtcacat tggaatcaac caatggcagc
     4561 gattacagca tcattgtgga aacatttgac gccaccgccg cgcagaccat cacgtttcat
     4621 ctgacaaacg gtttgtccca ggcgcaagtc aacgtttggg aaacgaccca aaccagccag
     4681 ttcataaaag tttcgcagct cactccgagc aatggctctt ataccttcac cttccaaccg
     4741 gaatgtctct acaccctgac caccaccacc ggtcaaacca aggggagcgc aactccgccg
     4801 gcggcaactc cgtttccgct tccttacaag gattcttttg aaaattatgc cgtggcgcag
     4861 acgccaaaat atttctcgga ccaggccggc acgttcgaga cgttcacgcg cgctgacggc
     4921 aaaggccaat gtctgcggca ggtcttgacg cgaattggca tccgctggac ttcggaatgg
     4981 caaccctaca cgttgctcgg cgatgccaac tggcaggatt atgatgtggc gtc
//
LOCUS       DASZMM010000068        13457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_842889, whole genome shotgun sequence.
ACCESSION   DASZMM010000068 DASZMM010000000
VERSION     DASZMM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13457
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842889"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VG754_01735"
     CDS             <1..985
                     /locus_tag="VG754_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWF17956.1"
                     /translation="RSRLWQEWRRNLAKISKPDASLRLAELVLAECETTDPTRKHVKL
                     FSTPAEAKRIVAPAPANAQTSELRMLLCDFHIHTNYSDGKLTVPEVVDFYGRLGFDCI
                     CITDHLADPRRLIGKLARLTNMTVAENQLDEYFNVIERERRRAWRNYGMIVLTGIEFN
                     KDGYTKKTSAHLLGIDLKAPIPSSLDLPETIAQIHAQGGLAVASHPHIMKSEWGKNTL
                     YLWENHEKFAPLIDAWEIANRNNIFDCVGMKRLPFLANSDFHKPKHIYSWKTLLYCEK
                     HADAIKECIRRNEHVSITLYREGNQYAMEMEPSREKIEPLVLHSALPLKSA"
     gene            1036..1109
                     /locus_tag="VG754_01740"
     tRNA            1036..1109
                     /locus_tag="VG754_01740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1068..1070,aa:Gly,seq:tcc)
     gene            1738..3657
                     /locus_tag="VG754_01745"
     CDS             1738..3657
                     /locus_tag="VG754_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWF17957.1"
                     /translation="MKIELISFIKSSHRITGSIVLTLVLTAMFASAAVASTSPNENPP
                     AYFLAAAKTEESQSASTSKPVDANAEHAKLFVENKYPSAATCRTCHPNHYREWSVSAH
                     AYAQISPVFNAFQATVTKLTQGANGDFCIRCHTQVGMNLGEPIYMSNMDRHPTSREGI
                     TCIVCHRLRNDYGKVSGRFALVTGDILTPVYGPTGDTELKRVLSMPDVYRVVTNETEA
                     GRKIHTDINQFAALRTSGFCGSCHDVTLQNGFRLEEAFSSFKNSPAAHRGESCQDCHM
                     GLIPGKKSGYAEEPAAIVGGVPTKPRRRTNHMFAGPDYSVVNRGFFPHNDKAAALATM
                     REWLTFDDKAGWGTDEFENHVPKDYKFPPRWTSVDDRYDARAILNDQYKLLADIQKQR
                     LTILRQGYQLGDLVVEQANSKGLKFKIQVKSGTDGHNVPTGFDAERISYMQVFVTDPA
                     DKSVFKSGDLDPNGDVRDLHSSYVEEGKLPLDPYLFSLQSRFLTSNVRGGEREQVLAI
                     NYSVDPLPFIRPMPFAMNFTGRAAGARIQRRGIEALSSRWANYKVKASELTGPGPYKV
                     RIRFLAGMVPVNLISEIKVAGFDYNMSPREVADAIKNGQSLLYDKEITLQLDGKKPTI
                     NLAAVADAPASYAQK"
     gene            3704..5122
                     /locus_tag="VG754_01750"
     CDS             3704..5122
                     /locus_tag="VG754_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17958.1"
                     /translation="MLFLAALSAAEADDTNSVDANAKDTARFSDQPAPLDLATFPERP
                     APLIEWGDKFLGNGNIQKGITLPTGEVISPNFWVFGTYRTGIQTFDNGGGASSRITEW
                     ANRLDIFANLQLSATERVLVGWRPLDRFRPDGTTDYTGYRFEPQSSQGFREAFSSTPR
                     ALFFEGELGEIFPKLDEHDKKSLDYGFSVGRQPLTLQDGILANDNSVDMVTVTRNSLL
                     IPGGSTLRLSGLFGWGQIERQRYSLASVPNAQDSGALLTGLDGAADFPLSTVEGDLLY
                     ESSSSYGNEFMAGIGSIQRIGKLNTTFRVNTSVAIDAQNKQATSGTLFTAHLSYTLPS
                     SVDLIYIDGFWGIDRFSSAARDPSAGGPLGAIGILTAAPQLGVYGAPLSNQADHSAGG
                     SLGYQIFFDELRRKQLIIELGGRAPTQTPTLLRQQSAGGIGVRYEEAFGRRFVLVLDT
                     FGVVRDQSAASVGGRVEWLVKF"
     gene            5270..6406
                     /locus_tag="VG754_01755"
     CDS             5270..6406
                     /locus_tag="VG754_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWF17959.1"
                     /translation="MDGELFSERLALARLQLARRENSGSWNGTRKFQIERIVRECENV
                     SSFYLVPHDKRPLPTFLPGQYLTFEMNIPGQRNRTVRCYSLSDRPRPEYYRVTIKRIT
                     SLADDPKSKPGLVSNFFHSALKAGDILDVKAPSGGFYLEMHRLTPVVLLAGGVGITPM
                     ICMANEITALTPQREIWLFFCSRNSEEHIMKEQLEQLARTNSNLHLHICYSQPHASDV
                     KGKDYQHEGRLNLVLLQMTLPSNNFDFFMCGPGAMMSDLNEGLRVWGVPDENIHMEAF
                     GPASVKRAVSPASKPPGQQIKVKFSRSNREIAWYGQPDSLLDLALAEGVPIGYGCRAG
                     NCGSCKTAVKSGKVKYLKQPGCDVEAGSCLTCIAVPECDLSLDA"
     gene            6464..7480
                     /locus_tag="VG754_01760"
     CDS             6464..7480
                     /locus_tag="VG754_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWF17960.1"
                     /translation="MVKITRSLLVPMFALCLLHVARAAEDMYHADPAKIMGPEACNEC
                     HAPMVEAWKLTHHYDTFNTMHRRPEAKDIATKLDIRRIKDESLCLKCHYTDKTDDTGK
                     VTPISGISCESCHSAAKDWVKIHSAKDDPDRLAKAEKLGMLRPNNYYHVAANCFSCHT
                     IPEEKLVNVGGHKAGSDFELVSWLSGEVRHNLQKSAGKVNEEIPVERRRMLYIVGRAL
                     DLEYSLRGMAVATEDGAYSKAMLARINSATDRLGEIAKASDLAEVKNMVAAVNPAALK
                     PGNSEVILKMANTVGDISAGLAKNQDGGKLASIDSLMPGPDKYKGTAYTPPAGAGGGT
                     PAHP"
     gene            7568..9313
                     /locus_tag="VG754_01765"
     CDS             7568..9313
                     /locus_tag="VG754_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWF17961.1"
                     /translation="MSEDSQSIKRPQRWDIPFWREMADTLGESAIQPDVDRLLQIPVF
                     RQMDPGRFPSNTSLREILLNDGRIRRFKRGDIIVRQGDYGSSAFIVLSGSAKVILDKL
                     SDAATGRQEPVRKSVFGAFAQWWQNSTLPEVRDLHQHSSNRQPGHTDIINRNEAAVFV
                     QDISAIIKGKRSVDLPAGELFGEIAALSRTARTTTVIADADSEMLEIRWQGLREIRLH
                     APEFKDYVDRLYREHNLENHLRETAMFRHLDDAALKRVAAATEFLTFGNFDWYATYSK
                     IREASASERIQKEPVVAHEGHYPNGVYLIRSGFARLSETYNNGERTVSYLGRGQIYGF
                     EEVALNWMKKTELPFQRTLRALGYVDVLFVPTSILEQQVLPSLPPTDLPRSQTGQKAH
                     RSRTGSHRSKIPDEMMEFIVERRFINGTAAMIIDMDRCTRCDDCVRACASTHDNNPRF
                     LRHGPIQDGFMIANACMHCQDPVCMIGCPTGAIHRDSAHGRVVINDLTCIGCATCANS
                     CPYDNIQMVTARSADGRPYYDHATNQPIQKAAKCDLCASQITGPACFNACPHDALVRM
                     DMREVDTLADWINQR"
     gene            9364..10119
                     /locus_tag="VG754_01770"
     CDS             9364..10119
                     /locus_tag="VG754_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17962.1"
                     /translation="MFAFLLDYALRVHLRRAELVSGLTLFAFIVILTLFNARKKLPFL
                     PLLRASTWLQIHIYAGLLSCVMFGLHVGWKLPRGVFESILAVLFCLVSASGLAGLAFS
                     RWSPGRLTVHGENVIFERIPALRTALRHEVEEMVADSIAKSQSSTISDYYETKLRNYF
                     SARRYLWHHLLGNNKPLFKLLSEAQALDRYFNAEERVIMARIVEKIQAKNNLDFQQVS
                     QGLLKGWLFVHIPLTYAMILAATVHGIMAWKFS"
     gene            10433..12262
                     /locus_tag="VG754_01775"
     CDS             10433..12262
                     /locus_tag="VG754_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17963.1"
                     /translation="MMAMGAMLAFLLMALGSNRGKRTFSPGSLSFAHRSVNDNCSECH
                     AALPAHPAKWLTTVAGAHSVHDNSALCLNCHNVGKAPLQPHSLGLLNLQPLTTASLKK
                     NGPGNTPLSLALASFIPSDSHSADQGIACATCHKEHHGKETDLKKITNNQCQNCHATQ
                     FASLSEGHPQFTRYPFERRTRIIFDHEAHLREHFRDPAVAKFSPTSCLDCHQTDLRGG
                     TMVVKSYETACSSCHDNQIKAKSAAKTGIPFIGIPRMDDRTLTGDNSIGEWPEDADQP
                     LTPFLQLILSSEPQLREALGKLQGVDLSNLPKADSEKLKAAQTLAWGIKSLILDLGSL
                     GQDELIRRINLCSGHTLTDYEQEGIVAFLNADALRSTFRTAFPNLQKEVLDYRKKGKA
                     APTQLIPSPELPAAGQVKNVSPDVWVSQGGWYSPDGTFTLYYHPRGHRDRFLSSWMDL
                     TVDDSHALDPAISRALFKTLSEPKAVGLCSKCHSIDDKPAKRVNWVGSRPDPLEHEFN
                     RFSHSAHLSLLDARGCFTCHTMKLTGSQTKDTYASAFEPGHQDPSQFQSNFRTIDKAV
                     CANCHQPSLVRDDCLLCHNYHIGRFKPVIPHATIAARPQPIGN"
     gene            complement(12564..>13457)
                     /locus_tag="VG754_01780"
     CDS             complement(12564..>13457)
                     /locus_tag="VG754_01780"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HWF17964.1"
                     /translation="WGDGGGFGGSDSLGRVSLGFARIEDGPENFRGVNINGGLNAEHA
                     ASFPKKGKASGLVCVNGILYANINLQDGKWPDVHHLMAWSTNHGATWAKADWMFGKRA
                     GEFQPSRFLNFGRDYSGVPAHLAGYVYLYGFKQPAKGDETKELYLARVAKDKILERAA
                     YDFFAGCDEKNRPKWNSDFAAAMPVLADTRGITPCSAVYDAAIKRYLAASFHVGPGQL
                     GIFDAPQPWGPWTTVAYHENFGRMGNAGEGLGCEFPRKWMSEDGLTLWCVFSVYGGSA
                     KQGINAHDRFNLVKVTLETTR"
BASE COUNT         3143 a         3782 c         3492 g         3040 t
ORIGIN      
        1 tcgctcgcgt ctctggcagg aatggcggcg caacctggcc aagatcagca agcccgacgc
       61 ttcgttgcgc cttgccgaac ttgtgcttgc cgaatgcgaa accaccgacc cgacgcgcaa
      121 gcacgtcaag ttgttttcga caccggcaga agcgaaacgg attgtcgctc ccgcgcccgc
      181 caacgcgcag acctccgagt tgcgcatgtt gttgtgcgat ttccatattc acaccaatta
      241 ttccgatggc aagttgaccg tgccggaagt ggtggatttt tatggacggc ttgggttcga
      301 ttgcatctgc atcaccgatc acttggcgga cccgcgccgg ttgatcggca agctcgcgcg
      361 cctgaccaac atgaccgtcg ccgaaaacca actggacgaa tatttcaacg tgatcgagcg
      421 cgaacgccgc cgcgcctggc gcaattacgg gatgatcgtc ctgaccggta tcgagttcaa
      481 caaggacggt tacacgaaaa aaacttcggc gcatctgctc ggcattgatt tgaaggcgcc
      541 gattccttcg tcgctcgatt tgccggaaac catcgcgcaa atccacgcgc agggcgggct
      601 cgcggtcgcc tcgcatccgc atatcatgaa aagcgaatgg ggcaagaaca cgctttacct
      661 ttgggaaaat cacgaaaaat ttgcgccgtt gattgacgcg tgggaaatcg ccaatcgcaa
      721 caacatcttc gactgcgtcg gaatgaaacg cctgccgttc cttgccaaca gcgatttcca
      781 caagcccaag cacatctatt cttggaagac gcttctctat tgcgagaagc acgccgacgc
      841 catcaaggaa tgcatccgcc gcaacgagca tgtctccatc acgctttacc gcgaaggcaa
      901 ccagtatgcg atggaaatgg aaccgagccg cgaaaaaatt gaaccgcttg ttctgcattc
      961 cgccctgccg ctgaagtcgg cctgagaacg catccgattg acaatccgca gtcaggcggt
     1021 aatatcccga aggctgcggg tgtagctcaa tggtagagtt ccagtcttcc aaactggcca
     1081 cgcgggttcg attcccgtca cccgctccat tttcagaaca ttttgtattt caatgcagtt
     1141 aatcacttca agctacgcgc agcgctcttc tgtcataaca ttctcataac accgccttgc
     1201 gagacaaagg ccaagccagt tgccaaacac gcggacgctc accccatctc agcctggtcg
     1261 tgattgacct tgtcgccagc gattgcgccc ctgcactccc ccgcaacact ggctaaaagt
     1321 tcgacaccac caggatgcct tacgcgcagc gaacggtttc ggcgatgaca tcggcacatt
     1381 cccccgaaat aaaccattcc cggttggtcg acgaatttgg gaagcgactt caagccgttt
     1441 cgtgtgggag agcccttgcg tgcggcaaac tgtcttttaa gagcacgggt gcttgcgtgc
     1501 caattgacac gatgtgatac cctttgacgc ggctggtgtt caataccggg caaagtggag
     1561 gcggcattca tggccaggtt tctttgggat ggaaagcact ttttccttta cttttttcgg
     1621 gtagatgttt taacagcgga actcggatgg aagcaatgcc cacaagtctt tccaaatgcg
     1681 cattgctgaa ggcgcgaaga atatctgacc ctgtttgtgc tgaaagaagt tgcgcgtatg
     1741 aaaatcgaac ttatttcatt tatcaagagt tcacaccgca tcactggcag catcgtgctg
     1801 acccttgtgc taactgcaat gttcgcctca gccgccgtgg cgtcgacttc acccaacgaa
     1861 aatccacccg catatttttt ggcggcagcg aaaactgaag aatcgcaatc ggcatccaca
     1921 agtaaaccag tggacgcgaa cgcggagcat gccaaattat ttgtcgagaa caaatacccg
     1981 tccgcagcca cctgccgcac ctgtcaccca aaccattatc gcgaatggtc tgtgtcggca
     2041 cacgcctacg cgcagataag cccggtgttc aatgcatttc aggcgaccgt caccaaactg
     2101 acccagggag cgaatgggga tttctgcatt cgatgccaca cacaggtggg aatgaatctg
     2161 ggtgaaccca tctacatgag caatatggac cgccacccga cctcccgtga aggcattact
     2221 tgcattgtct gccatcgcct cagaaatgat tacggcaaag tcagcgggcg cttcgcgctg
     2281 gtcacgggag acattcttac gccggtttat gggcctactg gtgatacgga acttaagcga
     2341 gtgttgtcca tgccggatgt ttatcgagtg gtgaccaatg aaacggaagc gggtcgcaaa
     2401 attcacactg acatcaatca atttgccgcg ttgcgcacct cggggttctg cggttcctgc
     2461 catgacgtga cgttgcagaa tggttttcgc ctggaagaag ctttcagctc gttcaaaaac
     2521 tcgcctgcgg cccatcgcgg tgaatcctgc caggattgcc acatggggtt gattcccgga
     2581 aaaaaatccg gttatgcgga ggagcctgcc gccatcgtcg gcggggtgcc gaccaagccg
     2641 cgccggcgca ccaaccacat gtttgccggg ccggattatt ccgttgtgaa ccgtggtttt
     2701 ttcccgcaca acgacaaggc ggcggcgctg gccaccatgc gcgagtggct cacattcgac
     2761 gacaaggcgg gctggggcac ggatgaattt gagaatcatg tgcccaagga ctacaaattc
     2821 cccccgcgct ggacttcggt tgacgatcgt tatgatgcga gagccatcct gaacgaccag
     2881 tacaaactgc tggccgatat acaaaagcaa cgcctgacca ttctacgcca aggctatcag
     2941 ctcggcgatt tggtggtcga gcaagccaat tcgaagggcc tcaaattcaa gatccaggtc
     3001 aagagcggca cggacgggca taatgtgccg accggtttcg atgccgagcg tatctcgtat
     3061 atgcaggtgt tcgtcaccga tccggcggac aagtcggttt tcaaatcggg agaccttgac
     3121 cccaacggcg atgtgcgcga cctccattcg tcttatgtgg aggaagggaa attgccgctt
     3181 gatccgtatc ttttctcgct tcaatcccgc ttcctcacga gcaacgttcg cggtggggaa
     3241 cgggaacaag tgttggccat caactattcg gttgacccgc tgccgttcat ccgtccgatg
     3301 ccatttgcga tgaacttcac cggccgtgcc gctggcgcgc gcatccagcg gcggggcatc
     3361 gaagccctgt ccagccgttg ggccaattac aaggtcaagg cttcggaatt gaccggcccg
     3421 ggcccataca aagtcagaat ccgatttctc gccggaatgg tgccggtgaa tcttatcagc
     3481 gaaatcaagg ttgccgggtt tgattacaac atgtcgccac gcgaggtggc cgacgccatc
     3541 aaaaatggac aatcgctgct atacgacaag gaaatcactc tgcagctcga cggaaaaaaa
     3601 ccaaccatca acttggccgc agtagccgac gcgccggcaa gctatgctca gaagtaaact
     3661 gcccagaaca cactacattc atcgcaggat tggaatcaca gggttgttgt tcctggccgc
     3721 attgagcgcc gccgaggccg acgataccaa ttctgttgat gccaatgcca aagataccgc
     3781 ccgtttctcg gaccagcccg cgccgctgga tttggcgacc tttccggaac gaccggcacc
     3841 actcatcgaa tggggcgaca agtttttggg gaacggcaat attcagaagg ggattacgct
     3901 gccgacaggc gaagtaatat ctcccaattt ttgggttttc ggcacatatc gcacaggcat
     3961 ccaaacgttt gataacggcg gtggagcatc gagccgcatc acagaatggg caaaccgcct
     4021 ggatattttc gccaatctgc aattatccgc caccgagcga gtgttggtcg gctggcgtcc
     4081 gcttgaccgg tttcgtcccg acggaacaac agactatact gggtatcgtt tcgaaccgca
     4141 atcttctcag ggtttcaggg aggccttcag cagcacgccg cgcgccttgt ttttcgaggg
     4201 tgaattgggt gaaatttttc ccaaactcga tgaacacgac aagaagagct tggactatgg
     4261 tttttccgtc ggcaggcagc ccttgacact gcaagacgga attcttgcca atgacaatag
     4321 cgtggacatg gtgacggtca ctcgcaactc ccttcttatc ccgggtggtt cgacattgcg
     4381 gctttccggc ttgttcggct ggggacaaat tgaacgccaa cgctattcct tggccagcgt
     4441 gccaaatgca caggactcgg gtgccctgct aactggtctg gacggcgcgg cggattttcc
     4501 gctcagcaca gtcgagggcg acctgctgta tgaatcatcc agctcctacg gaaacgagtt
     4561 catggcgggt atcggttcca tccagcggat tggcaagctc aacacaacat ttcgtgtcaa
     4621 tacgtccgtg gcgattgacg cgcaaaacaa acaggcaacc agcggcactt tgttcactgc
     4681 ccacctgtcc tatactctgc cgtcatcggt ggacttgatt tatattgatg gattttgggg
     4741 tatcgatcgt ttcagttcag cggctcgcga ccctagcgcc gggggaccgt tgggagcaat
     4801 cggcattctc actgccgcac ctcagcttgg cgtatacggt gcaccgctca gtaatcaggc
     4861 tgaccactct gcaggtggta gcctcggcta tcaaatattt tttgacgaat tgaggcggaa
     4921 acaattgatt attgaactcg gcggtcgcgc gcctacgcag acacccaccc ttctgcgcca
     4981 gcagtcggcc ggaggtattg gtgtccgtta tgaggaggct ttcggacggc gattcgtgct
     5041 ggtgcttgac accttcgggg ttgtccgtga tcaaagtgcg gcatcagtag gcggtcgggt
     5101 tgaatggttg gttaaatttt agaaagcctg tcacatcatt aattgtatga atccagtaga
     5161 aatactcgct cccgccggtt ttattttgtt cctgttcgtg acgattaatc tcgcgttgct
     5221 gtcctataca accatgagac gcgccattgc catcggcgtc caacgtcgca tggacggcga
     5281 attattttcc gagagattgg ccctcgcccg cctccaactc gcgcgccggg aaaacagcgg
     5341 ctcatggaac ggaacccgca aattccaaat tgagcgaatt gttcgtgaat gtgagaatgt
     5401 cagctctttc tatttggtac cgcacgataa gcgaccattg cccacttttc ttccaggcca
     5461 gtacctgact ttcgaaatga acatacccgg ccagcgcaac cgcactgtcc gatgttattc
     5521 gttatccgac cggccccgcc ccgagtatta tcgcgtcacg attaagcgca tcacttcgct
     5581 ggctgatgac ccaaagtcca agccgggact ggtttcaaat ttctttcatt ctgcgctcaa
     5641 ggcgggggac atattggatg tcaaggctcc cagcggcggc ttttacctgg agatgcaccg
     5701 cctaacgccc gtggtactcc tcgctggcgg ggtgggaatt actcccatga tatgcatggc
     5761 caatgaaata actgcgctga ctcctcagcg ggaaatctgg ctctttttct gctcgcgaaa
     5821 cagcgaggaa cacatcatga aagagcaact tgaacaactg gcgcgaacga acagtaattt
     5881 gcatcttcat atctgctact cccagccgca tgcctcggat gtaaaaggca aagattatca
     5941 gcatgagggg cgtcttaacc tcgtcctgct gcaaatgaca ttgccatcca ataattttga
     6001 ttttttcatg tgcggtcccg gagcaatgat gagcgatttg aacgaagggc tgcgggtgtg
     6061 gggcgtgccc gacgaaaata tccacatgga ggcttttggt ccggcgagtg tcaagcgcgc
     6121 ggtttcgcct gcatccaaac ctccgggcca gcagattaaa gtcaaatttt cccggtcaaa
     6181 tcgcgaaata gcgtggtacg gtcagcctga ttccttgttg gatctggcct tggctgaggg
     6241 agtgcctatc ggctacggtt gccgagctgg aaattgcggc agttgcaaga ccgccgtcaa
     6301 atccggaaaa gtgaaatatc tcaaacaacc cggatgcgac gtggaagctg gcagctgcct
     6361 cacctgcatt gctgttccag aatgtgattt atcgttggat gcttgacaac aatgacctgt
     6421 caaacgttga ctagatgaaa ggcccaaaat taaacaaatc cgtatggtaa aaatcacaag
     6481 aagtctgttg gttcccatgt ttgcgctttg cctgttgcat gtggctcgcg ctgctgaaga
     6541 catgtatcac gctgatcccg ccaagatcat ggggcctgag gcttgcaacg aatgccacgc
     6601 cccgatggtt gaagcctgga aactcaccca tcattatgac actttcaata cgatgcatcg
     6661 ccgcccagaa gccaaggaca tcgccaccaa actggacatc cggcgcatca aggacgaaag
     6721 tctgtgcctt aaatgccact acaccgacaa gactgacgac actggcaagg ttacacccat
     6781 ctccggtatt tcctgcgaat cttgtcacag cgcggccaag gattgggtta agatccatag
     6841 cgccaaagat gatcccgatc gtctcgccaa agcggagaag cttggcatgc ttcgtccgaa
     6901 caattattac catgtagctg ccaattgttt ttcctgccac accatacccg aggaaaaact
     6961 cgtcaatgtg ggtggccaca aggctgggag cgatttcgag ctggtctcgt ggctttcggg
     7021 agaagtccgg cataatcttc aaaaatctgc cggtaaggtc aatgaggaga ttcctgtcga
     7081 gcgccgccgc atgctctaca tcgttggccg cgccctggat ctggaatata gcttgcgcgg
     7141 aatggccgtg gcaacggagg acggcgctta ctccaaagcg atgctcgcaa ggattaactc
     7201 agccacggat cggctgggtg aaatcgcaaa agcttcggac ttggccgagg taaaaaacat
     7261 ggtggcggca gtaaatccgg ccgctttgaa gccgggaaat tccgaagtaa tcctcaagat
     7321 ggcaaatact gttggcgata tcagcgcggg cctcgccaaa aatcaggatg gcggcaaact
     7381 agccagcata gattctctca tgcccggccc tgacaaatac aaaggaactg cgtacacgcc
     7441 tcctgccggc gctgggggag gcacgccagc gcatccttga ttttggaagt cgttggttgt
     7501 gcgctgcggc gaagcgcagg cgcgcagtgc actgcaaaac tgaaatagcg agcgacattt
     7561 tttggccatg tccgaagatt cacaaagcat taaacggccc caacgctggg acattccttt
     7621 ttggagagaa atggcggaca ccctcgggga gtcggccatc cagccggatg tcgatcgctt
     7681 gctccaaatt cccgttttcc ggcaaatgga tcccggtcgg tttccgagca atacttcttt
     7741 gcgtgagata ctgctgaatg atggacgcat ccggcgcttc aaacgtgggg acataatagt
     7801 ccgtcagggc gactatggca gctcagcctt cattgtgctt tccggctctg caaaagtcat
     7861 cttggataaa ctcagcgacg cggcgactgg ccggcaggag ccggtccgca aaagcgtgtt
     7921 cggggctttc gctcaatggt ggcaaaactc gacgcttcct gaagtgcgag atttgcatca
     7981 acattcatcc aaccgccagc cgggccatac cgacatcatc aaccggaacg aggcggctgt
     8041 gtttgtccaa gacatcagcg ccataatcaa gggtaagcgc tcggtcgatt tgcccgcagg
     8101 cgaactcttc ggggaaattg ccgccctcag ccgaaccgcc cgaaccacga cggtcattgc
     8161 ggacgccgat tcggagatgc tggagatccg ttggcagggt ttgcgtgaaa tccggcttca
     8221 cgcgcctgaa ttcaaggact atgtggaccg gttgtatcgg gagcacaatt tggaaaacca
     8281 tttgcgcgaa acggcgatgt tccgccatct tgacgacgcc gccttgaaac gggtcgcggc
     8341 tgcgacagaa tttctgactt tcggaaattt tgactggtac gccacttatt ccaaaattcg
     8401 cgaggcaagc gcttcggagc gcattcaaaa ggagcccgtg gtggcgcatg aaggacatta
     8461 tcccaatggg gtctatctca ttcgcagcgg tttcgcgcgc ttaagtgaaa catataacaa
     8521 cggcgaacgc actgtgagtt atctaggccg gggacagatc tacggttttg aggaagtggc
     8581 attgaattgg atgaaaaaaa cagaactgcc atttcaacgg acactcaggg cgctgggtta
     8641 cgtggacgtt ttgtttgtgc ctacatccat tctcgaacag caggtcttgc caagcctgcc
     8701 gccgaccgat cttcccagat cacagactgg gcagaaagcc caccgctcaa gaacgggcag
     8761 tcatcgctcg aagattcccg acgagatgat ggagttcatc gtagaacgcc ggttcatcaa
     8821 cggcacagct gccatgatca tcgacatgga tcgttgcacg cgttgcgacg attgcgtgcg
     8881 cgcttgcgcg tcaacccatg acaacaaccc gcgctttttg cgacacgggc cgattcagga
     8941 tggcttcatg atcgcgaatg cctgcatgca ctgccaggac ccggtctgta tgatcggttg
     9001 ccccaccggc gccattcatc gtgattcagc ccatgggcgc gtcgtgataa acgatctcac
     9061 atgcatcggt tgcgccacgt gcgcgaacag ttgcccctac gataatatcc aaatggtgac
     9121 cgcccgcagc gccgacggtc gcccttatta cgaccacgcg acgaatcaac ccattcagaa
     9181 ggctgccaaa tgcgaccttt gtgcctcgca gatcaccggc ccggcttgct ttaatgcctg
     9241 ccctcatgat gcactcgtgc gaatggacat gcgggaagtg gacacccttg ctgattggat
     9301 caatcaaaga tgacactcac cacccgccga caattaggat ggtctctatt cgcggtcgca
     9361 accttgtttg cctttttgct cgactatgct cttcgcgtcc atcttagacg ggcggaactg
     9421 gtgagcggtc tgacattgtt tgcctttata gtaatactga cgctcttcaa tgcgcgaaaa
     9481 aaattacctt ttctgccgct cctccgcgcc tctacctggc ttcaaatcca tatttacgcc
     9541 ggattgctga gttgcgtcat gttcggtttg catgtgggct ggaagttacc acggggcgtt
     9601 tttgaatcga tactggcggt cttgttttgt ctggtttcgg ccagcggttt ggcgggcttg
     9661 gcgttttcac gctggtcgcc cggccgtctg actgtgcatg gtgagaacgt gatttttgag
     9721 cgcattccag cattgcggac ggcgttgcgg cacgaagtgg aagaaatggt cgccgactca
     9781 atagccaaat cacaatcttc gaccatttca gattattacg aaacaaaact caggaattat
     9841 ttttccgcgc ggcgctatct ctggcaccat ctcttgggca ataacaagcc gcttttcaag
     9901 ctgctttccg aggcccaggc gttggatcgc tattttaacg ccgaggagcg cgttatcatg
     9961 gcgcgcatag tcgaaaagat ccaagccaaa aacaacctgg attttcagca agtgagccag
    10021 ggtttactca agggctggct ctttgtgcat atcccgctga catacgccat gatcctggct
    10081 gcgacagttc atgggatcat ggcatggaaa ttttcataaa attcttccgc taatgcgccg
    10141 gcttcaggaa tttggcttcg agaaaagcca ttacgaaagg cccaaccaaa aatgggtttg
    10201 cgggcacgcg cacgagggcc aatgctgcgt cgccggcccg gacgcccatg gcaactgcac
    10261 cgcaacaacg gagtgccgcc cgttgcgaaa gggagatcgt tggtactgct cccggcccgc
    10321 ctttctcggc ggcccatgcg ccgaaggccc cttgcctgac ggtcaatgct gccgcgtgat
    10381 acccaaatgc agcccggtcc gaagtcttcg ctcgtggcgc ggtattactg ccatgatggc
    10441 aatgggagcg atgttggcat tcctgctgat ggccctaggt tcgaatcgtg gcaaacggac
    10501 gttttcccca ggctcattaa gcttcgctca ccgctccgtg aacgacaatt gctccgaatg
    10561 ccacgcagcg cttccggcgc atcccgcgaa atggctgacg accgtcgccg gagcccattc
    10621 cgtccatgac aacagcgctt tatgtttgaa ttgtcataat gtaggaaagg cgccgcttca
    10681 accgcattct ctaggactcc tcaacttgca accattgacc acggcttccc ttaaaaaaaa
    10741 cggacctggt aacaccccat taagtctggc actcgcctct ttcattccca gtgattcaca
    10801 ttcagccgac caaggaattg cctgtgctac atgtcacaag gaacatcatg gaaaggaaac
    10861 ggacttaaaa aaaataacca acaatcaatg tcaaaattgc cacgccactc aattcgccag
    10921 cctctctgag ggacatcctc aattcacccg ttacccgttc gagcgtcgca cgcgaatcat
    10981 attcgaccac gaggctcatc ttcgtgaaca tttcagagat ccagcggttg ccaaattttc
    11041 gccaacgtcc tgcctggact gccatcagac agatttgcgg ggaggcacga tggttgtcaa
    11101 atcatacgag accgcctgca gttcctgcca cgataaccag attaaagcca aatccgccgc
    11161 caaaaccggt attccgttca ttggcattcc gcgaatggac gaccgcacgc tcaccggcga
    11221 caattcgatt ggtgaatggc cggaagacgc cgatcaaccg ctgactccgt tcctgcaact
    11281 gatcctttca agcgaacccc agttgcgcga ggcgttgggc aaactccagg gcgttgacct
    11341 ctcgaatttg cccaaagcgg actctgaaaa actcaaggct gcccaaacgc ttgcctgggg
    11401 aattaagtcc ctgatcctcg acctcggctc gctgggacag gatgaactga tcagaaggat
    11461 taatctttgt tcggggcata cactcactga ctatgaacag gaaggcatcg tagccttcct
    11521 gaacgcagat gcattgcgct ccacgtttcg aaccgctttt cccaacctgc aaaaagaagt
    11581 gttggactat cgcaaaaaag gcaaggcagc ccccacccag ttgattccat ctccagaatt
    11641 acccgccgca gggcaggtca aaaatgtgtc ccccgacgtc tgggtgagcc aggggggttg
    11701 gtatagtccg gacgggactt tcactctcta ttatcacccg cgcggccatc gtgatcgctt
    11761 cttgagttcc tggatggatc ttacagtgga tgacagccac gctttggacc ccgcaatcag
    11821 tcgcgctctt ttcaaaactc tgtccgagcc caaggcggtc ggactttgct ccaaatgcca
    11881 cagcattgat gataaacctg ccaaacgggt caactgggta ggttcccggc ccgatccgtt
    11941 ggagcatgaa ttcaaccgct tctcccactc ggcacacctg agcctgcttg atgctcgggg
    12001 ttgtttcact tgccacacga tgaaactcac cggtagccaa acgaaggata cttatgcttc
    12061 agccttcgag ccaggccacc aagatccttc acaattccaa agcaatttcc gaacgattga
    12121 caaggcggtt tgcgcaaatt gccatcaacc gagcctggtt cgagatgact gcctcctttg
    12181 ccacaattat cacattgggc gtttcaaacc cgtcattcca cacgccacga tcgctgcgag
    12241 gccacaacca atcgggaatt agcacgtctg gaagtcatct cgtcaaagtc gtaagattca
    12301 tcaaccggca ctatgaattt tgtgccatat gctcgccatg ctgacgtaac ttggaaccaa
    12361 gcattcgttt gaactgttaa gaatctcata acacccagcg atggtcaaag tggttttatg
    12421 gttttgaaga atgttgcaaa taactagtga atatacgctt ttatcacaag cagccaaata
    12481 cgagaatctt gagaaagagc gatatggaaa cacgggttcg attcccgtca cccgctccat
    12541 ttcgcaaggt ttattcaccg caatcatcgg gttgtttcca aagttacctt gaccaagtta
    12601 aatcggtcat gggcgttgat gccttgcttg gcgcttccgc catacacgct gaaaacgcac
    12661 cagagcgtca gtccatcttc gctcatccat ttgcgcggga attcgcagcc aagcccttcg
    12721 cctgcatttc ccatccgccc aaaattttca tgataagcga ccgtcgtcca aggcccccac
    12781 ggttgcggtg cgtcaaagat gcccaactgg cccggcccga catgaaaact cgcggcgaga
    12841 tagcgtttga tggccgcatc atagacggcg gaacaaggcg tgatgccgcg cgtatcggca
    12901 aggaccggca tcgctgctgc gaaatcagag ttccatttcg gtcggttctt ttcatcgcaa
    12961 ccggcgaaaa agtcataagc tgcgcgttcc agaattttgt ccttcgccac gcgcgcgaga
    13021 taaagctctt ttgtctcgtc gcctttggcc ggttgtttga agccgtaaag atagacataa
    13081 cccgcgagat gggccggaac gccggaataa tcgcggccaa agttcaaaaa cctgctcggc
    13141 tgaaattcgc cggcgcgttt cccgaacatc caatccgcct tggcccaagt tgcgccgtgg
    13201 tttgtggacc acgccatcag gtgatgcacg tcaggccatt tgccatcctg caaattgatg
    13261 ttcgcataca aaattccatt cacgcaaacg agtccggacg ccttgccttt tttgggaaac
    13321 gacgcggcgt gttcggcgtt caagccccca ttaatattga cgccgcgaaa attttccggc
    13381 ccgtcctcaa tccgcgcaaa ccccaggctg acgcgtccca gagaatccga tcctccgaat
    13441 ccgccgccat cgcccca
//
LOCUS       DASZMM010000069         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1118972, whole genome shotgun sequence.
ACCESSION   DASZMM010000069 DASZMM010000000
VERSION     DASZMM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118972"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            499..1527
                     /locus_tag="VG754_01785"
     CDS             499..1527
                     /locus_tag="VG754_01785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWF17965.1"
                     /translation="MQIGVDSFAAQSVDNAGMEVSGSQSVRNLIQRIEIADQAGLDVF
                     GVGEHHRKEFLDSAPAVILGAAAARTKRIRLTSAVTVLSAADPVRVFQNFATLDLISQ
                     GRAEMVVGRGSFIESFPLFGLQLEDYDSLFAEKLDLLLKLRENEFIHWSGKHRAPLTG
                     QGVYPRPMQNPLPIWLGVGGTPQSFARAGMLGLPLMVAIIGGETRRFRPLVDLYREYG
                     RRAGHAPEKLKVGVHALGYVAETTEQAIDEFFPGYAEMFTRIGKERGWPPVTRAAFDA
                     VRGKHGALLVGNPDEVVEKILRHSEALGGISRITFQMDVVDMPQAKLLRSIELLGSRV
                     GPALRKKT"
     gene            complement(1588..3000)
                     /locus_tag="VG754_01790"
     CDS             complement(1588..3000)
                     /locus_tag="VG754_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWF17966.1"
                     /translation="MSLYAELFKESPQNGGEYGRAAVHCGDFELASQIWEKIRRLDPN
                     NIACIFWLAGEYAKIGLNGKARILLSRAAKIEPRNLEVRIRLAWLLSRTGSVEEARDV
                     VNQCLALDARNQATLYLNALLDRRENKLAAGEQQLRNLLVGGATAPNVRYSCHAELAH
                     ILDRTERHEEAMAQLETAKSEWRQNFESKPGHKDFFEWRENEIRQARALPKNILQTWA
                     KAFPSEVQVALPTIAFLTGSARSGTTLLEQIMDAHSSVAAVDESFAFHRIQPFVDVNA
                     PEIPVEQPNLWRQRYLKSLTLDLQLTRKETVFLDKNPSHTFWLPGFLRLFPELRVLIA
                     LRDPRDILISLYFQDQPYTNPLTLEQLAQHYSDVMELWLAVREWDGLAWMETRYEDIV
                     TDLQKEGRRVTEFLGLEWQDTQARYHEQNRDKKILSTNYNDVTKPVYNRSVGRWRTYE
                     KYLKPVLPMLEPYCKKFGYA"
     gene            3277..>3768
                     /locus_tag="VG754_01795"
     CDS             3277..>3768
                     /locus_tag="VG754_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17967.1"
                     /translation="MKTDQKICNYCGHENYVEAETCCGCGCTDWKPMIVAVPEKSPDV
                     LVPKAPLPKAFIPSPAITKEGSQTIIHCRTMAEVILVADQFEKADIITMVADDVEQTY
                     TIAVLTSAYEAAQELHDPVDPGFNRTFSEMPLRSDLKILALALPVFPPIAIFEMASLW
                     NRYT"
BASE COUNT          863 a          979 c         1031 g          895 t
ORIGIN      
        1 gtttctcgcc tttcaaaaaa cacgattttt tggcgtgtag gacaaaaagt ttgttcccta
       61 cacgcaaaaa ggggtgtttt tctgcaatca ggggagcgcg gctcgatgag ccgcagcaac
      121 gcgcgaccaa caggatatgc cgggctaatt catgtgcgcc catccttccc acattgctgc
      181 gggtcaccga cccgcgctcc gttccctatt cagagccaca gatggataca cacggattta
      241 aacggagaag gcgagacacg aattggcacg aattaacacg aattcaccca aaatgattcc
      301 catttgtgaa aattagtgta attcgtgtcg ttcatttttc ccgtttactg aggaaaggtg
      361 gtagaactaa taggtatgct caggcaagaa acatggtatt tcggcgggac actatcgccg
      421 tgatagtgtg tatgtatgac agaattactg gaaacgaacg aacgaagcca ggcggagcga
      481 ggaaggccga ctgacacaat gcagattggt gttgatagtt ttgccgcaca atctgtggac
      541 aacgccggca tggaagtcag cgggtcacaa agcgttcgca atttaatcca gcgcatcgaa
      601 atcgccgatc aagcggggtt ggacgtgttc ggagtcgggg aacatcatcg caaggaattt
      661 cttgattccg cgccggcagt catcctcgga gcggcggcgg cgcggacgaa acgcatccgg
      721 ctcaccagcg ccgtcacggt tttgagcgcc gcagacccgg tgcgcgtgtt ccaaaatttc
      781 gccactctgg acctgatttc acaaggccgc gcggaaatgg tggttggccg aggctcgttc
      841 atcgagtcgt ttccgttgtt tggtttgcaa ctggaggatt acgactcgtt atttgcggag
      901 aaactggacc tgcttttgaa gctgagggaa aatgaattca ttcattggtc cgggaaacac
      961 cgcgcgccgc tgacgggcca gggcgtttat ccgcggccga tgcagaaccc gctcccgatc
     1021 tggttgggcg tgggcgggac gccacaatca ttcgcgcgcg ccgggatgct cgggttgcca
     1081 ttgatggtcg ccatcatcgg cggcgagacg cggcggtttc gtcccctggt tgacctgtat
     1141 cgggaatatg gacggcgcgc gggacatgcg cccgaaaagc tcaaagttgg tgttcacgcg
     1201 ctggggtacg tcgccgaaac gacggaacag gcgattgacg aatttttccc cggttatgcc
     1261 gagatgttca cccgcatcgg caaggaacgc ggctggccgc ccgtcacgcg tgcagctttc
     1321 gacgccgtgc ggggcaagca tggggccttg ctggttggca atcccgacga ggtcgtggaa
     1381 aaaatcctac gtcacagcga agcgctcggc ggcatttcac ggatcacttt ccagatggac
     1441 gtggttgaca tgccacaggc gaagctcctg cgctcgatcg aactgctggg cagccgcgtc
     1501 ggtcccgcgt tgcgaaagaa aacgtaaaag gcgcgactat gctgctgcgg ctgcttctgc
     1561 gaacacagcc gcgctcggtt ctctcgctta cgcgtagccg aactttttgc aatacggctc
     1621 cagcatgggc aaaactggtt ttaaatattt ttcgtaagtt cgccagcgcc cgacggacct
     1681 gttataaacc ggcttggtga cgtcgttgta atttgtggac aggatcttct tgtcgcggtt
     1741 ttgttcatga taccgcgcct gtgtgtcttg ccattccaag ccgagaaatt ccgtcacgcg
     1801 ccttccttct ttctgcaaat cagtcacgat gtcttcgtaa cgcgtttcca tccacgccaa
     1861 cccgtcccat tcccgcaccg cgagccagag ttccatcacg tcgctgtaat gttgcgcgag
     1921 ttgttcgagc gtgaggggat ttgtgtaggg ctggtcttga aaataaaggc tgatcaaaat
     1981 gtcgcgcgga tcgcgcaacg cgatgagcac gcgcagttcg gggaaaagac gcaaaaaacc
     2041 gggcaaccaa aatgtgtgcg acgggttctt gtccaaaaaa acagtctcct tccgcgtcaa
     2101 ttgcaaatcg agcgtcaagc tcttcaagta acgctgacgc catagattgg gttgttccac
     2161 cgggatttcc ggcgcgttga catcaacgaa aggctggatc cgatgaaacg cgaaggactc
     2221 gtcaacggca gccacggaag aatgggcatc catgatttgt tccagaagcg tcgtgccgct
     2281 tcgcgccgag ccagtcagga acgcgatggt tggcagagca acttggactt cagacggaaa
     2341 tgcttttgcc caagtttgca aaatattttt tgggagcgcc cttgcctgac ggatttcatt
     2401 ttcgcgccat tcgaaaaaat ctttgtgccc tggcttcgat tcaaaatttt gccgccattc
     2461 gctttttgcc gtttccaatt gcgccatggc ttcttcgtgg cgttcggtgc ggtcaaggat
     2521 gtgggccagc tctgcatggc aggaatagcg cacgttcggc gcggttgcgc cgccaacgag
     2581 caagttgcgc aattgctgct cgcccgcggc aagtttgttt tcccggcggt cgagaagcgc
     2641 attcagataa agcgtggcct ggttgcgcgc atccagcgca aggcattggt tgacgacgtc
     2701 gcgggcttct tcaacgctcc cggtccgcga cagcagccac gccaatcgga ttcgcacctc
     2761 caagttgcgc ggttcaattt ttgccgcgcg cgaaagcagg atgcgggctt ttccgttcaa
     2821 cccaattttt gcgtactcac cggcgagcca aaatatgcac gcaatgttgt tcggatcgag
     2881 gcggcggatt ttctcccaga tttgggaagc cagttcgaaa tcaccgcaat ggaccgccgc
     2941 gcgaccgtat tcgccaccat tttgcgggga ctctttgaac agttccgcgt acaatgacaa
     3001 tccttccgca ggtttattat tttttatgag ctgccgcgct ctttgcaatt tcagcgaggg
     3061 aacaggattc attacgcgcg cgcatcgtaa tgaagtcccg cgaataaatc tgtgcagccg
     3121 cgcctatatc ccgtgtaggg tttcaccgta cgaaagagtt atttgtggca cgtttagagg
     3181 cgtccgtaga atgcggtctg aattgcaaaa tgaaatctat ctgattagtc tcatggctgc
     3241 gtgtttcatc tgcgaaagcc aaaaaactct tgaagattga aaacagacca aaaaatttgc
     3301 aactattgcg gtcatgaaaa ttatgtagag gctgaaactt gctgcggttg tggctgcaca
     3361 gattggaaac caatgatagt agctgttccg gagaaatctc cagatgttct tgtaccaaaa
     3421 gctcccttgc cgaaagcttt catccccagt ccggcaatca cgaaagaagg ttcccaaaca
     3481 atcatacatt gtcgaacgat ggctgaagtg atacttgtcg cagatcaatt tgaaaaggcg
     3541 gacatcatca caatggttgc cgatgacgtt gaacaaacct acacaattgc tgttttgacg
     3601 agtgcgtacg aagccgcaca ggaattgcat gaccccgttg atcccgggtt taaccggacg
     3661 ttcagtgaaa tgccacttcg atcagatctc aaaattttgg ctcttgctct tccagttttc
     3721 ccacccatag ctatttttga aatggcgtct ctctggaatc gatacaca
//
LOCUS       DASZMM010000070         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2224431, whole genome shotgun sequence.
ACCESSION   DASZMM010000070 DASZMM010000000
VERSION     DASZMM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224431"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VG754_01800"
     CDS             <1..538
                     /locus_tag="VG754_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF17968.1"
                     /translation="KLTGPTIYCIWHNRLALSMAAYHGYIKEHSGGVGLAAMASASKD
                     GAMMSAVLEFSRVQPVRGSTSRRGPQALREMTTWARRNYDLAIIPEGPRGPRYMVQMG
                     VIALAQLTGFSIVPVTFNLDWKIAVKSWDRFQIPLPFSRCEMIFEKPFRVPREVSEKE
                     REAFRLQLEETLKKISRD"
     gene            complement(535..1503)
                     /locus_tag="VG754_01805"
     CDS             complement(535..1503)
                     /locus_tag="VG754_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17969.1"
                     /translation="MREFFLQGANRVLKIRVFSCVLIGAILSSSTLRAGDSAADQSAA
                     MEAQRQQAIADYTQKMKDANYPALFTKAAQEFNVPPDVLMGVSFAETRWTQLQWPPGE
                     TASPENGMPHPYGIMSLWDNDYFGHTLSQAAKLIGQDPDVLKTDAFQNMRGGAALLRQ
                     LYDQTSKPADAPNETQIESWRNAIAKYTGIPQPELSQGHALRVYEFMNQGFHEYGIEW
                     NAHPVNLGPMREEVAKIKAAALAAKSAQLVQAKSDATTSPTLAAPSLQTNITPVAAKP
                     SPAPIVADDNSAANRRQRWYLAGLMAALLAIAVWYLRSSKRTNRPA"
     gene            2009..2377
                     /locus_tag="VG754_01810"
     CDS             2009..2377
                     /locus_tag="VG754_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17970.1"
                     /translation="MKPEQKKALLAAIDAGTLGQGSVAGDEYLHNMREARVTKEGVAH
                     WVETCFCATPLAEERPYWEEFFELLNVKDAHARRNCRHENGTEPWACCDCDCTKRLEQ
                     KLQSTGEAFLKNLRDAGSKQ"
     gene            2407..>3506
                     /locus_tag="VG754_01815"
     CDS             2407..>3506
                     /locus_tag="VG754_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17971.1"
                     /translation="MSAATLGSFFPVGPLVAENSAANPSAKLALDPALAADWLKRWKE
                     RILDETKNRFCDHAMGENLGWLLSPMLNDFYYGYMATQDAEWVARFVDWADSWIKRGV
                     PDPGRFIGWPEKGSGIGQGNGLLMDSLLGEAMALRPVVLMAKEIQRIPALREKYADRA
                     NAWLELAGRNFEKWDSFDCWREVKEGGLWVVPEFGVDSATGKWTDSYKQRATTGFSNP
                     DNKQNLIALWLLAMHDVTGKPVYRDRAEKWFRLMKSRMRAQQGGKYVVWNYWDPAGPW
                     DYKPDGSTRHWVGVHPNGGYYAIDVEGIVAAYEHGIVFTKDDIQRLIATNRDFMWNQQ
                     MKGARFQRIDGGEPDPRWKDTPGKLWTALVPH"
BASE COUNT          769 a          946 c         1032 g          759 t
ORIGIN      
        1 gaagttgacc ggcccgacga tttattgcat ctggcacaac cggcttgcgc tcagcatggc
       61 cgcctatcac ggttacatca aagaacacag cggcggcgtt ggcttggcgg cgatggcgag
      121 cgcgagcaag gacggcgcga tgatgagcgc ggtgctggaa ttttcgcgcg tgcaaccggt
      181 gcgcggctcg accagccggc gcggcccgca ggcgttgcgc gagatgacca catgggcgcg
      241 gcgcaattac gatctcgcca tcattcccga agggccgcgc gggccgcgtt acatggtgca
      301 gatgggcgtc atcgcgctgg cgcagctcac tggtttttcc atcgtgccgg tcacgttcaa
      361 tctcgactgg aaaattgcgg tgaagagctg ggaccgtttc cagattccgc tgccgttttc
      421 gcgttgtgaa atgattttcg aaaaaccgtt tcgcgtcccg cgcgaggttt cggaaaaaga
      481 acgcgaagct ttccgcttgc aattggagga gacgttgaaa aaaatttcca gagattaagc
      541 aggacggttt gttcgtttac tcgagcgcaa ataccagact gcaatggcca gcagcgcggc
      601 catcaatcca gcgagatacc aacgttgacg gcgattcgcc gcggagttgt catcggcgac
      661 gatcggtgca ggcgatggtt tggcggcaac aggcgtgata ttggtttgga gcgaaggcgc
      721 ggcgagtgtg ggcgatgttg ttgcgtcaga tttcgcttga acgagttgag cagactttgc
      781 tgcgagtgcc gcggccttga tctttgcaac ctcttcgcgc attgggccga ggttcaccgg
      841 gtgcgcgttc cattcgatgc catattcgtg aaagccctgg ttcatgaatt cataaacgcg
      901 caaggcgtgg ccctggctca gttccggctg cggaatgccg gtatatttcg cgatcgcatt
      961 gcgccagctt tcgatttggg tttcattcgg cgcatcggcg ggcttcgaag tctgatcgta
     1021 aagctgccgc aacaacgccg cgccgccgcg catgttttga aatgcgtcgg tcttcaacac
     1081 atcggggtcc tgtccgatga gtttcgccgc ctgcgacaat gtgtggccga aataatcgtt
     1141 gtcccagagt gacatgatgc cgtagggatg cggcattccg ttttcgggag aagccgtttc
     1201 gcccggcggc cattggagtt gcgtccagcg cgtctccgcg aacgaaacgc ccataagaac
     1261 atccggcggc acgttgaatt cctgcgcggc cttcgtgaac agcgcggggt aattggcgtc
     1321 cttcatcttt tgggtataat cggcgatggc ctgctgacgc tgggcttcca tggcggcgga
     1381 ttggtccgca gcagaatcac ccgcgcgcaa cgtgctcgaa gacaatatcg cgccgatcaa
     1441 aacgcaacta aaaacgcgga ttttcaacac acgattcgca ccctgaagaa agaattcacg
     1501 catcaacatt cgaaatgatt ggacgataaa aattcgagca tgggagagcg gccacagatt
     1561 gcacggattc cacagaaaga attttcatct atgaaatctt tggctcaaat tgttttttca
     1621 tgagttagtt tgttataaac aggccgccgg atttttcaag caaagatgcg ccgcagtggc
     1681 cttacgggcg ttcacatcct tgccggccca gaccaggtgt tcccgagtgt cccgaccgcc
     1741 gcgcgagcgg ggcaaccctt tcagggttgt gaatcttttc ccatgctcac ctgggacaca
     1801 tctcataaat aatggagcgc ggctgtgtcc gcaggaccag ccgcacagca gttttggaca
     1861 ctcgggctat cgttgcttgc tgcggctggc gcttcacgca cagccgcgct ccattattta
     1921 tgaaaaatta aatccacatt cgacacattg aaaattcccg gcgcacaaac gataatccct
     1981 ttgtatgcgt tatctcgtca aagcccgcat gaaacccgaa cagaaaaaag cgctgcttgc
     2041 cgcgattgat gccggcacgc tcggtcaagg ttccgtcgcg ggtgatgaat acctccacaa
     2101 catgcgcgaa gcgcgcgtga cgaaggaggg cgtcgcgcat tgggtcgaga cttgcttttg
     2161 cgcgactccg ctcgccgaag aacgtccgta ttgggaagag ttttttgagc tgctgaacgt
     2221 caaagacgcc cacgcgcgcc gcaattgccg ccacgagaac ggaaccgaac cgtgggcctg
     2281 ctgcgattgc gattgcacga agcgccttga gcaaaagctt caatcaaccg gcgaagcgtt
     2341 cttgaaaaat ttgcgggacg caggctcgaa acaatgaacc ggcgcgcctt ttttaaaaga
     2401 agcttcgtgt ccgccgcaac tctcggttcg tttttccccg tcggtccgct cgtcgcggaa
     2461 aattccgccg caaacccatc agcaaaactg gcgcttgatc ccgcgctcgc cgcggattgg
     2521 ttgaaacgct ggaaggaacg cattctcgac gaaaccaaga atcgtttttg cgaccatgcg
     2581 atgggcgaaa atctcggttg gctgctgagt ccgatgctga acgattttta ttacggttac
     2641 atggccacac aggacgccga atgggtcgcg cgctttgttg attgggctga ttcctggatc
     2701 aagcgcggcg ttccggaccc gggcagattc atcggctggc cggaaaaagg ttccggcatc
     2761 gggcagggca acggtctgct catggacagc ctgctgggtg aagcgatggc cttgcgcccg
     2821 gttgtcctga tggcgaaaga aatccaacga attcctgcgc tccgcgaaaa atatgcggac
     2881 cgcgccaatg catggctgga acttgccgga cggaatttcg aaaaatggga ttcgttcgat
     2941 tgctggcgcg aagtcaagga gggcggtttg tgggtcgtgc cggagttcgg tgttgattcg
     3001 gccactggca aatggactga cagttacaag cagcgcgcca ccaccggatt ttccaatccc
     3061 gacaacaaac aaaatctcat cgcgctttgg ttgcttgcga tgcacgacgt caccggaaag
     3121 ccagtatatc gcgatcgcgc cgagaaatgg tttcggctga tgaaatcgcg aatgcgcgcg
     3181 cagcaaggag gcaaatacgt tgtctggaac tattgggacc cggctggccc gtgggactac
     3241 aagcctgacg gttcaacgcg gcattgggtg ggcgtgcatc ccaacggcgg ttattacgcg
     3301 attgacgtgg aaggcatcgt cgccgcttac gagcatggga ttgttttcac aaaggatgac
     3361 atccaacggc tcatcgccac caaccgcgat ttcatgtgga accagcagat gaaaggcgcg
     3421 cgctttcagc gcattgacgg cggcgaacca gacccgcgct ggaaagatac gcctggaaaa
     3481 ttgtggaccg cacttgtccc gcatga
//
LOCUS       DASZMM010000071         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3867879, whole genome shotgun sequence.
ACCESSION   DASZMM010000071 DASZMM010000000
VERSION     DASZMM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3867879"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VG754_01820"
     CDS             <1..954
                     /locus_tag="VG754_01820"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWF17972.1"
                     /translation="LIEHAEAGARPVRPNKPLNIFLGIVIGLVVGVGLAFFIEYLDTS
                     VKTIDDVERTLQSPVLGVIPQNVGYLIEEGAESPHAEAYRVLRTNLLFSRKDDKLNTI
                     AVVSAGAGEGKSTTVCNLATVFAQSGARVLMVDSDLRRPTLHKLMGVTNNLGLTNYLL
                     KQNALEEVIQTSKLQNLDFMASGKLPSSSLGILSSAQMKDLISELKRRYDFVFFDSPP
                     IMGVSDASILASEVDMTLQVIQYRRYPQPMNIRAKQLIEKVGGNLIGIVLNNINMSQD
                     ESYYYYSGYYHDYYSKNEDETAPLTNGAPAEKSRVDIKPKY"
     gene            1038..1658
                     /locus_tag="VG754_01825"
     CDS             1038..1658
                     /locus_tag="VG754_01825"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HWF17973.1"
                     /translation="MAALFLAGCETDNAKYSYDPLAQNAAPASSGGLASTAPMVPPAP
                     VDASGTTMHIGDSVTFNFSDVPADLKPFDDTVKEDGSVTLMYNEKFQADGKTIGSLQQ
                     EIHDRYVPKYYRYLTVTIKPQERFYYVGGEVRSPNRQVYTGRMTVLQAIDTAGGFTEF
                     ARKGKVHVTRANGQTFDEDCIKALKHPELDHEIFAGDKIQVVKRPW"
     gene            1658..2047
                     /locus_tag="VG754_01830"
     CDS             1658..2047
                     /locus_tag="VG754_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWF17974.1"
                     /translation="MVQLKIVSGKMAGTEQVARHFPFRIGRSASADLRVEEDGVWDEH
                     LELAFHSTDGFILTAHPNALAAINGQPFREAVLRNGDAIEIGALKIRFWLGATRQSRL
                     GLREWLTWTAFALIIAAQLFLIYRMIP"
     gene            complement(2082..2543)
                     /locus_tag="VG754_01835"
     CDS             complement(2082..2543)
                     /locus_tag="VG754_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF17975.1"
                     /translation="MNESTATASKHIMVIEDAGSVADLIREMLSGTVYCVEICMTVED
                     ALAKFAPKKYDLIITDYTMPRMNGIELAHVLRRQAPEQCILLITGSTFSMLEDGQEYP
                     VNAFLQKPFSMAEFQDSMTALLKDSAPSVTEPEAQIPPRDSKTKNSRRGGI"
     gene            2729..>3320
                     /locus_tag="VG754_01840"
     CDS             2729..>3320
                     /locus_tag="VG754_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17976.1"
                     /translation="MLTRGDVFFKILAKLTKIKSYYSRQPRIHFACIANLLIFAVGVT
                     GCIPTTRHEPPQTAGKRTNSQGKVVQRIIREVTRTYHPVLLTPEGPKTRVESRCKFFF
                     QEGETPRRAFLIGNSATNQFFGDCLAVKDSALWVTFWCEPMLIFTTNADLTVDDRKRD
                     DLHVLIFDEKGFMRHRIFTTTSKQGWDEDYTDSAKAQ"
BASE COUNT          806 a          898 c          852 g          764 t
ORIGIN      
        1 ctcattgaac acgcggaagc cggtgcgcga cctgtgcgac cgaacaagcc gctcaacatt
       61 ttcttgggca ttgtcattgg ccttgtggtg ggtgtgggct tggccttttt cattgaatat
      121 ctggatacca gcgtcaagac cattgacgac gtcgagcgca ccctgcaatc gccggtattg
      181 ggtgtcattc ctcaaaacgt cggttatctc atcgaggaag gcgcggaaag tcctcatgcc
      241 gaggcttacc gcgtgctgcg cacgaacctg ctgttctccc gcaaggacga caagctgaat
      301 acgattgccg tggtcagcgc cggcgctggc gaaggcaaat ccacgaccgt ttgcaacttg
      361 gccactgtct tcgcccaaag cggcgcgcgc gtgctcatgg tggattcaga cctgcgacgc
      421 ccgaccctgc acaagctcat gggcgtgacc aacaatcttg gattgaccaa ctacctgctc
      481 aagcagaatg ccttggagga agtcatccag accagcaagc tgcaaaacct cgatttcatg
      541 gcgagcggca agttgcccag cagttcgctc ggcatcctta gttcagcgca gatgaaggat
      601 ttgatcagcg aactcaaacg tcgctatgat ttcgtcttct tcgattctcc gccgatcatg
      661 ggcgtgagcg acgcctccat cttggccagc gaagtggaca tgaccctcca ggtcatccaa
      721 taccgccgtt atccgcagcc gatgaacatc cgcgccaagc aattgatcga aaaagtgggc
      781 ggcaacctca tcggcatcgt tttgaacaac atcaacatgt cccaagacga gagctattat
      841 tattacagcg gttactacca cgattattat tcgaagaacg aggatgaaac cgcgccgctt
      901 acaaacggag cacccgcgga aaaatcgcgc gtggacatca aacccaaata ctaatcaaac
      961 gcaaacaatc aaatctgatt caatacgcaa gcatggaact tatgaaattt aggggattcg
     1021 ccgggttggc agccaccatg gcggccctct ttctggccgg gtgtgaaacg gacaatgcca
     1081 aatacagtta cgatccgctg gcgcaaaatg ccgctccggc aagcagcggc ggccttgctt
     1141 ccactgctcc catggttcca ccggctccgg tggatgcgag cgggaccacg atgcatattg
     1201 gtgacagtgt gacgttcaac ttctcggacg tgccagccga cctcaaacct tttgacgaca
     1261 ctgtgaagga ggatggctcc gtcacgctca tgtacaatga gaagtttcag gccgacggca
     1321 agactatcgg ctcgttgcag caggaaatcc acgaccggta tgttcctaaa tattacaggt
     1381 atttgacggt caccatcaaa ccccaggaga ggttttatta tgtcggcggc gaagtccggt
     1441 cccccaaccg ccaagtttat acaggacgga tgaccgtgct gcaggcaatt gataccgcgg
     1501 gcgggttcac cgagttcgcc agaaaaggca aggtgcatgt gactcgcgcc aatggccaaa
     1561 ccttcgacga ggattgcatc aaggctttga agcacccgga actcgatcac gaaatttttg
     1621 caggcgacaa aattcaggtg gtcaagcgcc cttggtaatg gtccagctca agattgtttc
     1681 cggcaaaatg gcgggcaccg aacaggttgc ccgccatttt ccttttcgca tcggacggtc
     1741 cgcatcggcc gacttgcggg tcgaggagga tggtgtttgg gacgagcact tggaactggc
     1801 atttcattcc accgacggat tcatccttac cgcgcatccc aatgcgctcg cggcgatcaa
     1861 cggtcagcct tttcgtgaag cggttttgcg caacggcgat gccattgaaa tcggcgcgct
     1921 caaaatccgc ttttggcttg gggcaacccg ccagtccagg ctcggcctcc gggaatggct
     1981 gacttggacc gcgtttgcgc tcatcatcgc agcccagttg ttcctcatct accggatgat
     2041 tccctgaaac ccttcttcac cacgccggca agaaaggatt tttaaattcc accacgacgg
     2101 gaatttttcg ttttggaatc gcgcggagga atttgtgctt ctggctccgt gacggatggg
     2161 gccgaatctt tgagcagcgc cgtcatcgaa tcttgaaatt ccgccatgga aaaaggtttt
     2221 tgcaaaaagg cgttcaccgg atattcctgg ccgtcctcca acatggaaaa agtcgagccg
     2281 gtgatgagaa ggatgcattg ctccggcgct tgtctcctga ggacatgcgc cagttcgatt
     2341 ccattcatcc tcggcatggt gtaatccgtg atgatgaggt cgtatttttt cggcgcaaac
     2401 tttgccagcg cgtcctcgac ggtcatgcaa atctccacgc aatacactgt gcccgacagc
     2461 atttcccgga tcaaatccgc cactgaaccg gcgtcttcaa tcaccatgat atgtttggaa
     2521 gcagtcgctg tgctctcatt cataacggct ccatgcgaat tggagtaaca tatcttataa
     2581 gcagagtggc aacaagccgc ccctcctgca acaaaaattc cggcgttgcc tatttcccgg
     2641 gaattttgtg tccttaccaa ggactgataa acattaagga tgtattttca tttttcatca
     2701 atctgaccgc gttgcccatt cacagattgt gttgacccga ggcgatgttt tttttaaaat
     2761 cctcgcaaaa ttgacaaaaa taaagagtta ctattcacga cagcctcgca tccatttcgc
     2821 ttgtattgcg aatcttttaa ttttcgctgt aggggtgact ggctgtattc caacaacacg
     2881 ccatgagccg ccgcaaacag ctggcaagag aacaaactcg cagggcaaag tggttcagcg
     2941 gattatccgg gaagtcaccc gaacgtacca tcctgtgctg ttgacacctg aaggccctaa
     3001 aacaagagtg gaatcacgct gtaagttttt tttccaggaa ggcgaaacac cgaggcgggc
     3061 atttctgatt gggaacagcg ccacaaatca gttctttgga gattgccttg ctgtcaagga
     3121 cagcgccctg tgggtgacct tctggtgcga gccaatgttg atcttcacga caaacgcaga
     3181 tcttacggtt gatgacagga aaagggacga tctgcatgtc ctcatttttg acgaaaaggg
     3241 attcatgcgc catcggatat tcaccacgac atcaaaacag ggctgggacg aagattacac
     3301 agattcggcc aaggcgcaga
//
LOCUS       DASZMM010000072         7005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2225270, whole genome shotgun sequence.
ACCESSION   DASZMM010000072 DASZMM010000000
VERSION     DASZMM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225270"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..229)
                     /locus_tag="VG754_01845"
     tRNA            complement(153..229)
                     /locus_tag="VG754_01845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Gly,seq:gcc)
     gene            complement(286..1428)
                     /locus_tag="VG754_01850"
     CDS             complement(286..1428)
                     /locus_tag="VG754_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF17977.1"
                     /translation="MDLTRTAYGTWSGGRYMHFGEPLNEERFLKTIQLAWQKGIRTFM
                     TADVYGAGAADEMLGRALAGMPRDSYCLVGAVGHDFYKGERSGAKGYPRFTATQTPGE
                     YASYLRMATEKSLARCRAEKFDLLLLHNPDFTGYTSDKVWNAMEKVRDAKLTDRLGVA
                     PGPANGFTLDVILCFERFGPILDWAMIILNPLEPWPGRLVLPAAVKQDVNLITRVVDY
                     GGLFHDDVKPGHQFGAQDHRTFRPAGWVEAGAAKMEKMRDIAGKHQLTMLQFACAWNL
                     SQPGVKSVIPTLIQEVGANAKPIEEKVEELAALPEVSLNEEELEFIAEIGDNAGCMEL
                     KGSNRAHVGEPLPDRWGITRDHELLARKWNIDPDADLACTHKTAPA"
     gene            1726..2436
                     /locus_tag="VG754_01855"
     CDS             1726..2436
                     /locus_tag="VG754_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine diphosphorylase"
                     /protein_id="HWF17978.1"
                     /translation="MRFEPGDLFDLKQTEHAELFDGCAHAWDALKALKGYIESHVRHA
                     PHSHEKARVFIGDDVVIGEGTIIEDGAMIKGPAIIGRNCEIRHNAYIREHVIIGDHCV
                     IGNSCEFKHSFLFNHSLVPHFSYVGDSILGYKSHLGAGVKISNVKLMPGNVKVLVDNA
                     PFDTGLRKFGALVGDQTDIGCNSVLNPGSIIGRGSIIYPCTNWRGILPANSIVKNRGG
                     QEITERWADGQAGLKSKK"
     gene            2626..4254
                     /locus_tag="VG754_01860"
     CDS             2626..4254
                     /locus_tag="VG754_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF17979.1"
                     /translation="MLLMEDMRLLQAYVTEGSEEAFKTILDRHVNLVYSTALRQVGDP
                     SLANEVTQTTFIILARKAHRLGQGTILAGWLYRTAQFAATRALRTEARRREREREAAL
                     MQTETSGSIWEQLSPILDQAMADLGATDRNALVLRYFENKTAKDVGLALGLNEAAAQK
                     RLVRAIEKLRIFCTKKGVVLSGVALAGLLSANAVQSAPVSVTTATAAALHVGALGGST
                     GALTTGTLKLIAWTRLKIAMLTGLGVTTVVLGTVAFFHFTNAEPRYQDRVLTSWLEQL
                     DNRQDNFSAVLPWISWQSQWQPSAKQVEAAEAIRAMGTSAVPSLIAALEKTNLSRFDR
                     LFGRSNDLIEKNHRKAALALDALGPVCKPWVPQLSRILYQAQCPKEAAVALAAIGPEG
                     WDVLTKATTAKGDTVACAIWALGSHHITAPETEDALMASYERNYPSGMEIVSLWALME
                     IGSDHQKLIPLLTEGLHSPRSDMRWGSAVLLGRLGPEAGNALPALWLALQDKDPVVRH
                     DAAQAIEQIDPQVAAQANLPDALANIHVPKTSLF"
     gene            complement(4388..6274)
                     /locus_tag="VG754_01865"
     CDS             complement(4388..6274)
                     /locus_tag="VG754_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HWF17980.1"
                     /translation="MSESILAVDKAVPGTKPTIHLRRYPIWIAIIAAAAILAIGPGRA
                     LAQRPLGIDVSSYQGQPNWSSVHGGGVVFAYAKATEGTGITDGDFVYNQNNGKAAGVY
                     MGAYHFAHPNSASPGSEAGHFWGVAGPYVLNDGRSLMPTLDFEVFSGVVGASSYSDWA
                     NQWCNDIVSDGAAQDVSLKPVIYVSACSACNFNTSVATWHSWIADYNGENLYTGTPWS
                     TCTGCEVWGGGVWNMWQVSSSGAIPGISGNVDLDTFNGNTAGLTATLLVTSSCNLSLV
                     GGAIRAKYDSLGDCSSFLGYPTTPEEGTPDGIGRYNHFSNDGSIYWTPETGAWSIHGN
                     IQDHWASLGWETSPLGYPYTDETGTPDGIGRFNHFTNLNNGDVGSIYWTPQMGAWSIH
                     GNIQTHWASLGWETSFLGYPITDETGTPDGVGRFNHFTNLNNGDVGSIYWTPETGAWS
                     IHGAIRSHWASLGWETSPVGYPVTDETSTPDGIGRYNHFTNMVQGDMGSIYWTANYGA
                     WSIHGVIRTHWENLGWETSVLGYPVTDENGTPDGIGRFNHFENPRLNNQGGSIYWTSS
                     TGTCEVHGPIRTYWANAGWEKSSFGYPTSDQYAVTGTTNLVRNNFQHSTITLNTTTGV
                     VSSP"
     gene            6482..>7005
                     /locus_tag="VG754_01870"
     CDS             6482..>7005
                     /locus_tag="VG754_01870"
                     /inference="COORDINATES: protein motif:HMM:NF020927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HWF17981.1"
                     /translation="MKALLDKIARREIMLGAAVGSGMSAKAVESGGADFLIALSAGFY
                     RMQGRSSMLALLPYANANELTWQIASEQIMPCLERIPVVLGLCAQDPGLNPREQFSRI
                     KRHGMVGVTNFPSVVYFDGEYRAALERAGLGFEREVALLSQARENGLLTIGFCLTEDE
                     AVALCRANVHILCLG"
BASE COUNT         1569 a         1896 c         1954 g         1586 t
ORIGIN      
        1 acaggggtgg ggtgatcgag tgaaccggcc ggtatcatga acaccaaaga ttttatccgt
       61 ctcggcgggt cgttgggcaa ggccacaacc gattttgtca gcgattcatt ctgggaggcg
      121 ttggaagcag tgaagaaaaa ccttgaaaaa attggagcga gcgaagggat tcgaaccctc
      181 gacactcacg ttggcaacgt gatgctctac caggctgagc tacgctcgct tccgttcagt
      241 ctgccaaaaa tacgggaacc cgcccgaatt gcaagccctc tttttttaag ccggggcggt
      301 tttatgcgtg caggccagat cagcgtcagg atcgatgttc catttcctgg cgagcagttc
      361 gtggtcgcgc gtgatgcccc aacggtcggg caacggctcg ccgacatgcg cgcgattgga
      421 gcctttcaat tccatgcagc cggcgttgtc gccaatctcc gcgatgaatt cgagttcctc
      481 ctcgttcagg ctgacttcgg gcagtgcggc aagttcctcg accttttctt caatcggttt
      541 cgcattcgcc ccaacttcct ggatcaacgt ggggatgacg cttttgacac cgggttgcga
      601 gaggttccac gcgcaggcaa attgcagcat ggtgagctga tgcttgccgg caatgtcgcg
      661 cattttttcc atctttgcgg cacccgcctc gacccagcca gccggacgaa aggtgcgatg
      721 gtcttgcgcg ccgaattgat gacccggttt cacgtcatcg tgaaacaaac cgccgtaatc
      781 caccacgcgc gtgatgagat tcacgtcctg cttcaccgct gcgggcagga caagacgtcc
      841 gggccatggt tcgagcgggt tcaagatgat catcgcccaa tccaaaatcg ggccaaaccg
      901 ttcgaagcaa agaatcacgt ccaatgtgaa gccgttggcc ggtcccggcg cgacgccgag
      961 gcggtcggta agttttgcgt cgcggacttt ttccattgcg ttccaaacct tgtcgctggt
     1021 gtagccggtg aaatcgggat tgtgaagcag cagcaaatca aatttttccg cgcggcagcg
     1081 ggcgagcgat ttttctgtcg ccatgcgcag ataagaagcg tattcgccgg gcgtttgcgt
     1141 ggccgtgaag cgcggatagc ctttcgcgcc ggaccgttcg cccttgtaaa aatcgtgccc
     1201 gactgcgccg acgaggcaat aagaatcgcg cggcattcct gcaagagcgc gtccgagcat
     1261 ttcgtccgcc gcgcccgcgc cgtaaacatc cgccgtcatg aaggtgcgga tgcctttttg
     1321 ccaggcgagc tggattgttt tgagaaagcg ctcttcattg agcggttcgc cgaaatgcat
     1381 gtagcgtccg ccgctccagg tcccataagc cgttcgagtc aaatccataa atttaagatc
     1441 tgcaaaattg tttcaaattc attctacagt acatttcata atttaatgga gcgtgggccg
     1501 tgcgcgaagc gcccgccgca gcaatctacg aaaacccgaa tcttccaagc tgctgccact
     1561 ggtcctgcgg acacagtcgc gctcggttct ccacttgtaa ggtgacttgt aaccacagat
     1621 gaatgcagat taacgcaaag tgagcaggat ggaaatagtg tttagcgatt gggtgcttcc
     1681 cgaattcgat tgcgcgcgcc gcgcttttct ttaatttatt cgactatgag gtttgagccg
     1741 ggcgacttgt ttgatttgaa gcagacggaa catgccgaat tgttcgatgg gtgcgcgcat
     1801 gcgtgggatg cattgaaggc gctcaaaggc tacatcgagt cacatgtccg ccatgcaccg
     1861 cacagccatg aaaaggcccg tgtgttcatc ggcgatgatg ttgtcatcgg cgagggaacg
     1921 atcattgaag atggcgccat gatcaaaggc ccggccatca tcgggcgcaa ttgcgaaatc
     1981 cgccacaacg cctacatccg cgaacatgtc atcatcggcg accattgcgt catcggcaat
     2041 tcctgcgagt tcaagcattc gttccttttc aatcacagcc tcgtgccgca cttcagttac
     2101 gtgggcgatt ccatcctcgg ctacaagtca catctcggcg cgggcgtgaa gatttccaac
     2161 gtcaaattga tgcccggcaa tgtgaaagtg ctcgtggaca acgcgccgtt cgacacgggt
     2221 ttgcgcaagt ttggcgcatt ggtgggcgac cagaccgaca tcggttgcaa ctcggttcta
     2281 aaccccggca gcatcatcgg gcgcggttca atcatttatc cctgcacaaa ctggcgcggc
     2341 attttgccgg caaattcgat tgtgaagaac aggggtgggc aggaaatcac cgagcgttgg
     2401 gcagacggtc aagccggctt gaaatcgaag aagtgaaaca gttttacgga ttccgtgcgg
     2461 ctggaaagag ctgtttgggt cacaggcgtt tctggtacct ccttggcgcc tgctgaagtt
     2521 ttgtgtcttt gatgatatat gcccgcccaa atccaaaccg gacattttat tttaattttt
     2581 ttaatgtccg gttcccaatt ccggcgggca tactaatata gggcaatgct gttgatggaa
     2641 gacatgcggt tgttacaggc gtatgtgacg gaaggatcgg aagaagcctt taaaacgatt
     2701 ctggatcgcc atgtaaacct cgtctattca acggctttgc gacaagtggg cgatcccagt
     2761 ctggccaatg aagtgaccca gaccactttc atcattttgg ccaggaaggc gcacagactt
     2821 ggacagggga cgattcttgc cggatggcta tatcgaacgg cgcaattcgc cgcaaccagg
     2881 gcgctgcgga cagaagcgcg ccgacgggaa agggaacgcg aggcagcact catgcaaact
     2941 gaaacatcgg gctcaatttg ggagcaactt tcacccatct tggaccaagc catggcggat
     3001 ctcggcgcaa ccgaccgcaa tgctttggtc ctcagatact tcgaaaacaa aactgccaag
     3061 gatgtcggtc tggctttagg cctcaatgaa gctgccgccc aaaaacgact ggttcgcgcg
     3121 atcgaaaaat tacggatttt ttgcacgaaa aaaggggttg tgttgagcgg ggtggcgttg
     3181 gcgggattgc tttccgccaa cgctgtacaa tccgctcctg taagcgtgac tacagcaacc
     3241 gcagccgcct tgcatgtcgg cgcgctgggt ggttccacgg gagctttgac caccggcacg
     3301 ctcaaactca tcgcatggac caggttgaag attgccatgc tcactggttt gggagtgaca
     3361 actgtcgtcc tcgggacggt ggcatttttt cattttacca atgctgaacc gcgttatcag
     3421 gacagggtcc ttacgtcgtg gcttgagcag ttggacaatc gtcaagacaa cttttccgcc
     3481 gttttgccat ggatttcctg gcaatcgcaa tggcagccgt ctgccaaaca agttgaagcc
     3541 gcggaagcga ttcgcgccat ggggacttct gcagtgcctt ccttgatcgc agcgcttgaa
     3601 aaaacaaacc tgtcgagatt cgaccgtttg tttggacggt cgaacgattt gattgaaaaa
     3661 aatcaccgta aagcggcgct tgctttggac gccctcgggc ccgtctgcaa accatgggta
     3721 ccgcaattga gccgcatttt atatcaagcc caatgtccca aggaagccgc tgttgccctg
     3781 gccgcaatcg ggccggaggg ctgggacgtc ctgacaaaag ccacgaccgc caaaggcgat
     3841 acggttgcat gcgcaatctg ggccttggga agccatcata tcaccgcgcc ggaaactgaa
     3901 gacgcattga tggcaagcta cgagcggaat tatcccagcg gcatggaaat cgtgtccctc
     3961 tgggcattga tggaaatcgg ctcagaccat caaaaattga ttcctctgtt gactgaggga
     4021 ctgcattcac ccagaagcga catgagatgg ggctcggccg tattgctggg gcgtttgggg
     4081 ccggaagccg gaaacgcgct gcccgcatta tggcttgcct tacaggataa agatcccgtt
     4141 gtccggcatg acgccgcgca ggcgattgaa caaattgacc cccaagtcgc agcccaagcc
     4201 aatcttcccg atgcgctcgc gaatattcac gttcccaaga cctcgttgtt ttaaatactt
     4261 ataagaatgg tgacaaaaaa ggccgggaaa agtttcccgg ccttctgcat caaacacggc
     4321 caccacagcc cagcattgca cacacaaaat tagagctgcc gcagcgccgt tctgctggca
     4381 ttacggatta cggagaacta accactccgg ttgtggtatt gagcgtgatc gtgctgtgtt
     4441 ggaagttatt tcgcaccagg ttcgtcgtgc cggtaacagc atactgatca ctggtcggat
     4501 aaccgaacga acttttttcc cagcccgcat tcgcccaata ggtacggatc ggcccgtgaa
     4561 cttcacaagt tccagtcgaa gatgtccagt agatggatcc gccttggttg ttcaatctgg
     4621 ggttctcgaa gtgattaaac cgtccaatcc catcaggcgt gccgttttca tcggtgacag
     4681 gatagccgag aacgcttgtt tcccagccga gattttccca atgcgtgcgg atgacgccgt
     4741 gaatggacca cgcgccataa ttggcagtcc agtagatcga gcccatatcg ccctgcacca
     4801 tgttggtgaa gtgattgtag cgtccgatgc cgtcgggcgt gctggtttca tcggtcacag
     4861 gataacccac cgggctcgtt tcccaaccaa ggctggccca atgcgagcgg attgcgccat
     4921 gaatggacca tgcgccggtt tcaggtgtcc aatagattga gccaacgtcg ccgttgttca
     4981 gattggtgaa atgattgaaa cgtccgactc cgtcgggtgt gccggtttca tcggtgatgg
     5041 gataaccgag gaagcttgtc tcccaaccca ggctcgccca atgtgtctgg atgttgccat
     5101 ggatggacca cgcccccatt tgtggagtcc agtaaatgga accgacatca ccgttgttca
     5161 aattcgtgaa atggttgaaa cgtccgatgc cgtcgggcgt gccggtttca tccgtgtacg
     5221 gataacccag cggactcgtc tcccagccca agctggccca atggtcctgg atattgccat
     5281 ggatggacca ggcgccggtt tcaggcgtcc aataaatcga gccgtcgttt gaaaaatgat
     5341 tataccgccc gatgccatcc ggcgtgcctt cttccggcgt cgtcggataa cccaagaacg
     5401 aactgcaatc gcccagcgaa tcgtacttcg cgcggatagc gccgccgaca agggacaaat
     5461 tgcatgagga tgtcacaagc aacgtcgcag tcaggccggc tgtgttgccg ttgaacgtgt
     5521 ccaaatccac gttgccggaa atgccgggaa tcgcgccgct gctgctgacc tgccacatgt
     5581 tccagacgcc tccgccccaa acttcacaac cggtgcaggt gctccaagga gttccagtgt
     5641 acaaattctc gccgttgtaa tcggcaatcc aggaatgcca tgtcgccact gaggtgttga
     5701 aattgcaggc gctgcaagcg ctgacataaa tgaccggctt gagcgagaca tcctgcgccg
     5761 cgccatcgga cacaatgtcg ttgcaccact ggttggccca atccgaataa ctgctcgctc
     5821 cgaccactcc gctgaaaact tcaaaatcca gcgtcggcat caagctcctg ccgtcattca
     5881 gaacgtacgg gcctgccaca ccccagaagt ggcccgcttc cgaaccggga ctggcgctgt
     5941 tcggatgggc aaaatggtag gcgcccatgt aaacacccgc ggcctttcca ttattttggt
     6001 tatagacgaa atctccgtcc gtgatgccgg tcccttcggt cgcctttgca taggcaaata
     6061 caacgcctcc gccatgaacg ctcgaccaat tgggttggcc ttgatacgac gagacatcaa
     6121 tgcccagcgg acgttgcgcg agcgcgcgtc cggggccgat ggccaggatt gccgcagcgg
     6181 cgatgattgc aatccagatt gggtatcggc gcagatggat ggtcggtttt gttccgggaa
     6241 ctgctttgtc aacggcgagg atcgattcgc tcatatttgt tctttcttta tgggttgtgg
     6301 gttttggtgt gacatttgcg atggaacgga gaaacaaaag cagaacaaat gccacgaacc
     6361 tcacttgacg gaatcagcca taaatggccg aatctttgaa aaaattttgg ggcgctggct
     6421 gaatcttgtt gataagtgag taaaaaaagt gtaaacgctg tgaaccttac acaggttcca
     6481 catgaaagcg ctcctcgaca aaattgcgcg tcgcgaaatc atgctgggcg ccgcagttgg
     6541 ttcggggatg tccgcaaagg cggtcgaatc gggaggcgcg gattttctca tcgcattgag
     6601 cgccggtttc taccggatgc aaggccgcag ttccatgctg gcccttttgc cctacgcgaa
     6661 cgcgaacgaa ttgacctggc aaatcgccag cgaacaaatc atgccgtgcc tcgagcgcat
     6721 ccctgtcgtg ctcggcttgt gcgcccagga ccccgggctc aatccgcggg aacaattcag
     6781 ccggataaaa cgccacggca tggtcggtgt gacgaatttt ccttcggtcg tttatttcga
     6841 tggagaatat cgcgcggcgt tggaacgggc cggtcttggt tttgaaaggg aagtcgcgtt
     6901 gctctcgcag gcgcgcgaga acggactgct caccatcgga ttttgcctga cggaagacga
     6961 ggcggtggcg ctttgtcgcg cgaacgtgca catcctttgc cttgg
//
LOCUS       DASZMM010000073         8652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_844053, whole genome shotgun sequence.
ACCESSION   DASZMM010000073 DASZMM010000000
VERSION     DASZMM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844053"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="VG754_01875"
     CDS             complement(<1..1778)
                     /locus_tag="VG754_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF17982.1"
                     /translation="MVAILLVLLGPFVLLRTPTDIFPVINIPVVSIIWQFSGLSSDQM
                     EQRMVYPQERALTTTVNNIAHIESTSYDGVGVIKVFFQQGIDPVQGVAQITAISQTIL
                     KQLPPGTTPPLILQYSASTVPILEYAISSTSLSEQEIYDLTLNQVRVGLISVPGVGIP
                     LPYGGKVKVVSVDLDLKALEANNIAPSEVTAAINAQNLVVPAGTAKIGATEYDIDLNT
                     TPLVLNRLNELPIKIAEGTVILIRDVAQVHVGYLPQVNLVRFNGVPSTLLSVFKSGNA
                     STLQVASGVKAAMPKVQKTVTSALQVKQFADQSLFVSAAISGVVREMVIAALLTATMI
                     LLFLGSWRSTLIIAISIPLSILASLTMLSVLGETINLMTLGGLALAVGILVDDATVTI
                     ENIERHLQEGGTLEDGILKGAGEIALPALVSTCCICIVFVPMFLLGGVARYLFVPLAE
                     AVVFAVLASYVLSRTLVPTLLMYFERNVKHGNQNDQTSPPKNPEDDPKPHSSIISRLL
                     YPLIALQHHFEHGFERFRRGYGELLQSILSKRLVFATVFLAFCLGSWLLVPLLGQDFF
                     PSVDAGMFNLHVRTRTGTRIEQTSEI"
     gene            complement(1827..2987)
                     /locus_tag="VG754_01880"
     CDS             complement(1827..2987)
                     /locus_tag="VG754_01880"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HWF17983.1"
                     /translation="MAQPRSLQQTGAPVAQTLATIPPDNPQTPQKIALGEKLFFDGRL
                     SADGTVACATCHDPARAFTDGRPVSIGIHGLAGQRNSPTVLNALYNKTQFWDGRVMTL
                     EEQAELPIINPVEMGQTTLDAAVAALQRIKEYQDAFQTVFGRPPNGPDLRRAIASYER
                     TLLSFNSPFDRFMAGNANAIDDSAKRGWKLFNNQGRCNKCHALTDNQPNLTSFTDNDF
                     HNIGVGIIRHKVVPLARKAARLVNSGNSTAIDRAAIQSELSVVGRFLVTKKQSDIAAF
                     KTPDLRNVLITAPYFHDGSEQTLWDVMDHYNKGDGLQNPFLDQDIQPLALGENDINDL
                     VALMASLTSDDYKEAGAKELALQRAIANTKRPQRDTARAFGPKPVQPRPPGT"
     gene            complement(3136..3852)
                     /locus_tag="VG754_01885"
     CDS             complement(3136..3852)
                     /locus_tag="VG754_01885"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HWF17984.1"
                     /translation="MQTKLATYIHDQEAEPRIRDLLLGALAVSSGAIDSISFLALGKV
                     FSAFMTGNIVFLGFRVAGANAPSVATILTSIIAFAVGVYLSALIVKPTAGSGIWPARV
                     TVALAVSLIPHAVFLAVWFASNGQPSMAGVNILIGLWALAMGMQSAAVRTLHVEGVFT
                     TAATATVIVLASDISTWSITGAERRRLAGVLVSLFIGATAGGLLLVHAPIYAPVLPFV
                     ITVATVATAWTALRERNKSH"
     gene            complement(3952..5124)
                     /locus_tag="VG754_01890"
     CDS             complement(3952..5124)
                     /locus_tag="VG754_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate decarboxylase family bicupin"
                     /protein_id="HWF17985.1"
                     /translation="MNSQTKQRQRAQSANGPEPIRGNAGASVLGPRNLSREAENPDTI
                     ASPSTDHGSMGNMKWSFADSHMHLSPGGWGRQTTVHELPSAPEIAGVNMRLTAGGVRE
                     MHWHKAAEWAFMLKGRARITAIDQDGHAFQDDVGEGDLWYFAAGTPHSIQGIEGDGCE
                     FLLVFDDGNFSEEETFLLSDWMAHTPKEILAKNFGVPESAFARIPEKDLWIFQAETPG
                     PLAADRVAGTGPMSNTPVHRMMAQEPIRSSGGTVRITDSSNFPASKTIAAALVEIEPG
                     GMREMHWHPNADEWQYYLSGEARMTVFAAQNNARTFNFQAGDVGSIPFAMGHYIENIG
                     STTLRFLELFRSDHYADISLAQWLGFTPHELVRAHLNIDESVLAKIPQRKPLVVSG"
     gene            complement(5330..5926)
                     /locus_tag="VG754_01895"
     CDS             complement(5330..5926)
                     /locus_tag="VG754_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HWF17986.1"
                     /translation="MATKEKKSMIRKKRLCYALLLIWLATMIGRAQDTNEYVAPSEAV
                     PRGKAPRMQVQLLNPGESTKQYAVIFYQGDEAFSGLLEFAEKYQVTSAHFTAIGAING
                     ATLGWFDPRRKMYKKIPIKGQHEVIGMSGDIALYQGKPVVHTHMIVGAPDGTTRGGHV
                     LAVYASPTLEVMVTVDPVTMQKRFDPETDLTLIDPALK"
     gene            complement(6452..7798)
                     /locus_tag="VG754_01900"
     CDS             complement(6452..7798)
                     /locus_tag="VG754_01900"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWF17987.1"
                     /translation="MNLSALKAKIARKEIDTIVMVFPDAFGRLVGKRLTAHYFLEHAA
                     KDGTHGCNYLLTLNMEMEPLTGFKLANWEQGFGDFEMRPDFSTLRLLPWQPGAAMVIC
                     DLHHHGGKLVEEAPRSVLKRQLETLNAKGLVCKMASELEFFLFNDTYPAAFANKYSGL
                     TPASDYRIDYHTMQPARDEAIFRSLRNFMDAADVPVESSKGEWGKGQHEVNFIYDEPL
                     PMADRHLVFKQGAKEIVHQNGKCITFMSKIDAAEVGSSCHIHQSVWQKGKSLFWDLKK
                     RAGSKFFRQFLVGLMKYSPELTYFYAPTINAYKRYQSASWAPTKMAWAYDNRTVGFRV
                     VGERSSFRLENRMPGADANPYLAFAAMIAAGMAGVKENLDCGELYEGNAYLDPKLPSL
                     PRSLREAADLLDKSRLARNVLGEAVVDYYVHTARCEVQAFDDAVTDWERVRYFERI"
     gene            complement(7802..>8652)
                     /locus_tag="VG754_01905"
     CDS             complement(7802..>8652)
                     /locus_tag="VG754_01905"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWF17988.1"
                     /translation="LPPGALQVLAGSGEQMGDALVKHPLIRKISFTGSTSVGSHIMQL
                     AARDIKRVSLELGGKSPNIIFADCDLEKAAATSPMSVFANTGQDCCARSRIFVERSVY
                     EKFVELFVQATRKIVVGDPSSEDTQIGPLVSKQQRETVETFLPTAKTPHLKILCGGDR
                     PARKGYYLNPAVIVGCETSDRAWREEIFGPVTCIRPFEREEEMLAEVNASPYGLSGSL
                     WTNDLKRALRVSRRVESGVLSVNCHNSLHLEAPFGGFKQSGLGRDLGLAAMEGYTELK
                     NIYIAE"
BASE COUNT         1977 a         2427 c         2367 g         1881 t
ORIGIN      
        1 atgatctcag atgtctgttc gatgcgggtc cccgtgcggg tccgcacgtg caggttgaac
       61 attccggcat caacgctcgg aaaaaaatcc tggcccagca ggggaaccag caaccatgag
      121 ccaaggcaaa aagcaaggaa caccgtcgca aagaccaacc gcttcgacaa aatcgattgc
      181 aacagttcgc cataaccccg gcggaaacgt tcgaagccat gttcgaaatg atgctgcaag
      241 gcgatcagcg gatacaacag tcgagaaatg atcgaggagt gaggcttggg gtcgtcctcg
      301 gggtttttcg gcggtgatgt ttggtcgttt tgatttccgt gtttaacgtt cctttcgaaa
      361 tacatcagca aggttggcac gagagtgcgg gacaacacgt aggaagccag caccgcaaaa
      421 accaccgcct cagccaatgg cacaaaaaga taacgcgcca cgccgccgag caggaacatg
      481 ggcacaaaca caatgcagat gcagcaagtg gacacgaggg caggcaacgc gatttcaccc
      541 gcacccttga ggatgccgtc ttccagcgtc cctccctcct gcaagtggcg ttcaatattt
      601 tcgatcgtga ccgtcgcatc gtccaccagg atgccaaccg ccagcgccag cccgccgagc
      661 gtcatcaaat taatggtttc gcctaacacg ctgagcatgg tcaaggaggc cagaatcgaa
      721 agtggaatgg agattgcaat gatgagagtg ctccgccatg agcccagaaa caacaggatc
      781 atggtggccg tcaagagcgc cgcaatcacc atctcccgca cgacaccgga aatggcggcg
      841 ctgacgaaga gcgactggtc ggcgaattgc ttgacctgca aggcgctggt caccgtcttt
      901 tgaactttag gcatggcggc cttgactccg cttgcgacct gaagcgttga tgcgttgccc
      961 gatttaaaca cgctcaacaa agtgctgggc actccgttaa accggacaag atttacttgg
     1021 ggcaaataac cgacatggac ctgcgccacg tcacggatga gaatcaccgt cccttcagct
     1081 atcttgatcg gcagctcgtt cagccgattc agcacaaggg gagtggtgtt caaatcgatg
     1141 tcgtattccg tggcgccgat tttcgcggtg ccggcgggta caaccagatt ttgcgcattg
     1201 atggcggcgg tgacttccga aggcgcaata ttgttcgctt ccagggcttt caggtcaagg
     1261 tccaccgaaa ccaccttcac cttgccgcca tacggaagcg ggatgccgac ccccggaacg
     1321 ctgatcagcc cgacccgtac ctggttcagc gtaaggtcgt atatctcctg ttcggataga
     1381 gacgtgctgc tgattgcgta ttcaaggatt gggacggtcg aagcgctgta ctgaagaatc
     1441 agcggcggtg tcgttcccgg cggaagttgt ttcaggatgg tttgcgatat ggcagtgatt
     1501 tgcgccacgc cttgcaccgg gtcaatccct tgctgaaaaa aaaccttgat gactcccacg
     1561 ccgtcgtagg aagtggactc gatatgcgca atgttgttga ccgtggtggt cagcgcccgc
     1621 tcttggggat aaaccatgcg ctgttccatc tgatcggaac tcaggccact gaattgccag
     1681 atgatgctca ccaccgggat gttgatcacc ggaaagatgt ccgtcggagt gcgcaacaac
     1741 acgaacgggc ccaatagaac caatagaatc gcaaccacga taaacgtgta cgggcgattc
     1801 agggctaatc gaacaatcca cataactcag gtgccgggtg gtcgtggttg gaccggcttt
     1861 gggccaaagg ccctcgcagt atcgcgttgc ggtctcttgg tgtttgcgat ggcgcgttgc
     1921 agggctaatt ccttggcccc cgcttccttg taatcgtcac tggtcaacga agccatcaaa
     1981 gccaccaaat cattgatatc attctcgcct aatgcgagag gttgtatatc ctgatcgagg
     2041 aagggatttt gcaagccatc gcctttgttg tagtggtcca taacgtccca aagggtttgc
     2101 tctgatccat cgtggaagta aggcgcagtg atcagcacat tgcgcaggtc tggggtcttg
     2161 aatgcggcaa tgtccgattg tttcttggtg actagaaagc ggccaaccac actcagttcg
     2221 ctctggatgg cggcgcgatc aatggcggtc gaatttcctg aattgaccaa tcgggcggcc
     2281 ttgcgcgcaa gcgggacgac cttatgccgg atgatgccga ctccgatatt gtgaaaatca
     2341 ttgtcagtaa agctggtgag gttgggttgg ttgtccgtaa gcgcatgaca tttgttgcaa
     2401 cggccctgat tgttgaacag tttccaaccc cgcttggctg aatcgtcaat ggcattcgcg
     2461 ttgcccgcca tgaaccgatc aaacggggaa ttgaacgaca gcaacgtgcg ttcataagac
     2521 gcaatcgcgc gccgaaggtc tggaccattg ggaggacgtc cgaacaccgt ttggaatgca
     2581 tcctgatatt ccttgatgcg ttgtagcgcg gcaacggcgg catccaaggt ggtttgaccc
     2641 atttcaacgg gattgatgat cggcaattcc gcctgctcct ccaaggtcat cacccgtccg
     2701 tcccaaaatt gagtcttgtt gtacaaggcg ttcaagaccg tcggtgagtt gcgttgcccg
     2761 gccaggccgt gaatcccgat tgaaacaggt ctgccatcag taaatgcccg cgcaggatcg
     2821 tggcacgtgg cgcaagcgac ggttccatca gccgacaacc ggccatcgaa gaaaagcttc
     2881 tcgccgagag caatcttctg cggagtctgt gggttgtccg gtggaatcgt tgcgagtgtt
     2941 tgggcaaccg gcgcgccggt ctgttgcaaa gacctgggtt gcgccaaagg gccggcttct
     3001 ggaatggggg gcggatggcc tgcttgtgaa gccagagtga ccaaggatat ggacaaagca
     3061 aaaaaaagaa acttcttcca ggaccacggc aaaactttcg ttcgatgctg cattttttgg
     3121 cctcgacacc ggaaatcaat gactcttgtt tcgctcccgc aaggcggtcc atgctgtcgc
     3181 gaccgtcgca acagtgatca caaatggcag gacgggtgcg tagatgggcg cgtgaaccaa
     3241 aagcagacct ccagcagttg cgccgatgaa cagcgagacg agcacacctg caagccgtcg
     3301 ccgttccgca cctgttatgg accacgtgga gatgtcgctt gccagcacga tgacggtcgc
     3361 ggtcgcagcg gtcgtaaaca caccctcgac atggagggtc cgcactgcgg cgctttgcat
     3421 tcccatcgca agcgcccaca agccgataag aatgttgaca ccggccattg aaggttggcc
     3481 gttgctcgcg aaccacacgg ccagaaaaac agcatgcgga ataagcgata ctgcgagcgc
     3541 aactgtcacc cttgcaggcc agatgccgga acctgccgtg ggcttgacga tgagtgccga
     3601 caggtaaact cccaccgcga aggcaatgat cgaggtgaga atggtcgcga cactgggtgc
     3661 attggcgcca gcgacgcgaa atccgagaaa cacgatattt ccagtcatga aggccgaaaa
     3721 gactttgccc aaggcaagaa agctgattga gtcgatggct cccgacgaaa cagccagagc
     3781 gccgagcaac aggtcgcgaa tcctcggctc ggcctcctgg tcgtggatat atgtcgcaag
     3841 tttggtttgc atttaactcg gccttttcga caggttttgg ttcaaacgcg acgaagacag
     3901 ctcactatct gttgtggatg ctgcgccaac gatggccgcc ttgtccgcag gtcagccact
     3961 taccacgagc ggcttgcgct gcggtatctt ggccagcacc gattcgtcga tgttcaggtg
     4021 cgcccgcacc aattcgtgag gcgtgaaccc gagccactgt gccagcgaga tgtcggcgta
     4081 atggtcgctt cggaacaact ccagaaaacg cagcgtcgtg ctgccgatgt tttcgatata
     4141 gtggcccatg gcaaagggaa tggaaccaac atcgcccgcc tggaaattga aggtgcgcgc
     4201 gttgttctgc gccgcgaaca ccgtcatccg tgcttccccc gagagatagt actgccattc
     4261 atccgcattc gggtgccagt gcatttcgcg catgccgccg ggctcaattt caaccagcgc
     4321 agccgcgatt gtttttgacg cggggaaatt ggaggaatcg gtgatgcgta cagtgccgcc
     4381 actgctgcgg atcggttctt gtgccatcat ccggtgaacg ggggtgttag acatcggccc
     4441 ggtccccgcc acccgatcag cagcgagagg tcccggtgtt tctgcctgaa agatccagag
     4501 atccttctcc gggatgcgcg caaaagcact ttccggcaca ccaaagttct tggccagaat
     4561 ctctttcggg gtatgggcca tccaatcaga gagcaggaag gtctcctcct ccgagaaatt
     4621 gccgtcatcg aagacaagca ggaactcgca accgtcgccc tcaatgccct gaatcgaatg
     4681 cggcgtgccg gccgcgaaat accagaggtc accctcaccg acatcatcct gaaaggcatg
     4741 accatcctga tcaatggcag tgatgcgcgc ccggcccttg agcataaacg cccactcggc
     4801 cgccttatgc caatgcatct cccggacccc gccggccgtg agccgcatgt taacgccggc
     4861 aatctcagga gcgctgggca gctcgtggac tgtcgtctgg cgcccccagc cacctggcga
     4921 caaatgcatg tggctgtccg caaaagacca tttcatgttt cccatggaac cgtggtccgt
     4981 ggaaggagat gcgatggtgt cgggattctc agcctcgcgt gaaaggtttc gcggtccgag
     5041 gactgatgcc cccgcattac cccgaatggg ctcaggtcca ttggcggatt gggctctttg
     5101 acgttgttta gtctggctgt tcataagaat taaaataaaa cggtacgaat tagcatttac
     5161 gcgctgccgg tttgttgcgc tgcgtgggtg atccccaggt gaaactttct tggagcaaca
     5221 ggcggtcaag tcccttaccg ccaaccatcc cgaaaaacca gatttgttac aatagggtga
     5281 ataccccttt ggcgaacccg cagaaaacat gaaacgaact gcgataccgc tacttcaaag
     5341 ccggatcaat caacgtcagg tctgtctccg gatcaaaacg cttctgcatc gtcactggat
     5401 cgactgtgac cattacctcc agtgttgggg atgcgtacac ggcgagaaca tggccgccgc
     5461 gcgtcgttcc gtctggtgct cccacgatca tgtgcgtatg gaccacaggt tttccttgat
     5521 agagcgcgat gtcgccgctc atcccgatca cttcatgttg acccttgatg ggaatctttt
     5581 tgtacatttt gcgccgggga tcaaaccacc ccagcgttgc tccgttgatg gctccgatgg
     5641 cggtgaaatg cgcactcgta acctgatact tctccgcaaa ctccagcaat ccggaaaagg
     5701 cttcgtctcc ttgatagaag atgactgcat attgtttcgt cgattctcca ggattcagca
     5761 attgaacctg catccttgga gccttgcccc tcggaacggc ttccgacggg gctacatatt
     5821 cgtttgtgtc ctgcgcgcgc ccaatcatgg tggcaagcca aattagtaac aacgcataac
     5881 aaaggcgctt ttttcggatc atggatttct tctcctttgt tgccaactat ccagataaat
     5941 aaatttacaa caatggagtg aacacccctt gggacctgca taacgctttc caagttgacg
     6001 gatccaattc agggcgtgca acgaagcgct cggtttgatg tatgaagtga ccaatgatcc
     6061 cgcgcttttg aatgagatga tttgttacgc cgatcaaatt cggcactgcg aaacgacacc
     6121 gcaaacggac gcgtcatgtg aacaggcagg cgcgaaccgg tgtggcttac gaaaccttcc
     6181 gataaacggg ccggtcaccg cgacggaaat gagttcgttc aaaagtttca tgttaggcga
     6241 gacgccgccg aacggtcaga cttacgacaa ttcgcttacg gacacactca ccggcaataa
     6301 tgcgtgaaaa gagcggcatg ggcattcacc tgagcggaaa gggcctcaaa gcaccccaga
     6361 ttccgtggaa cacttccgct ccgaatcgta aactcctcga catctttttt gatcacctcg
     6421 tcgagcgctg atttgccttt gctcatgcgg gtcaaattct ctcgaaatac cggacacgtt
     6481 cccaatcggt caccgcatcg tcgaacgcct gcacttcgca acgcgcggtg tggacgtaat
     6541 aatcaaccac cgcttcgccc aagacgttgc gggcgagccg gcttttatcg agcaggtcag
     6601 ccgcctcgcg caaggagcgg ggcaatgaag gaagcttggg atccaagtag gcatttcctt
     6661 catacaattc gccgcaatcc aaattttcct tcacgccagc catgcccgcc gcaatcatcg
     6721 ccgcgaacgc gaggtaagga ttggcatccg cgccgggcat tcgattttcc agccggaagg
     6781 aactcctctc gcccaccacg cggaaaccga ccgtccgatt atcataagcc caagccatct
     6841 tggtcggcgc ccaactggcc gattgatagc gtttgtacgc attgatggtc ggggcataga
     6901 aataagtgag ttcgggcgaa tatttcatca gcccgacgag aaattgccgg aagaactttg
     6961 aacccgcgcg ctttttgagg tcccaaaaga gagacttgcc cttctgccag acgctctggt
     7021 gaatatggca actgcttcct acttcggcgg cgtcaatctt tgacatgaaa gtgatgcact
     7081 tgccattttg gtggacgatt tccttcgcgc cttgcttgaa aaccagatgc cggtcggcca
     7141 tgggcaacgg ctcgtcataa ataaaattca cttcgtgctg tcctttgccc cattcaccct
     7201 tggacgattc caccggcaca tccgccgcgt ccatgaaatt acgcaaggag cggaaaatgg
     7261 cctcgtcgcg cgccggttgc atcgtgtgat aatcgattcg ataatcactg gctggagtca
     7321 gcccggaata tttattggca aaggccgccg ggtaagtgtc gttgaacaga aaaaattcca
     7381 gttcgctcgc catcttgcac accagacctt tggcgttgag tgtttccagt tggcgcttca
     7441 acaccgagcg cggagcttcc tcgaccaatt ttccaccgtg atgatgcaaa tcgcatatca
     7501 ccatcgctgc gccgggctgc cagggtagca gccgcaacgt ggaaaaatcg gggcgcattt
     7561 caaaatcgcc gaagccctgc tcccaattcg ccagcttgaa gccggtcaat ggttccattt
     7621 ccatgttcag cgtcaggaga taattgcacc cgtgcgttcc gtcctttgcc gcgtgttcca
     7681 aaaaataatg cgcggtcagg cgcttgccga cgagccgtcc gaacgcgtcg ggaaaaacca
     7741 tgacaatggt gtcaatctcc ttgcgggcaa tcttggcttt gagcgctgac aggttcattg
     7801 gttattccgc gatgtaaata ttcttaagtt ccgtgtaacc ctccatcgcc gccaagccga
     7861 ggtcgcggcc gaggccgctt tgcttgaagc cgccgaacgg cgcttccaag tggaggctgt
     7921 tatggcagtt cacgctcaac acgccgctct caacccgcct tgacacgcgc agcgcgcgct
     7981 tgaggtcatt cgtccaaagc gaaccgctca aaccgtaggg tgaagcgttg acttccgcca
     8041 acatttcctc ttcgcgctca aaaggccgga tgcaggtgac cgggccaaaa atttcctcac
     8101 gccaggcgcg gtctgaagtt tcgcacccca cgatcaccgc cgggttgaga taataacctt
     8161 ttcgcgccgg gcggtcgccg ccgcagagaa tcttgaggtg cggcgttttt gcggttggta
     8221 aaaatgtctc cacggtttcg cgctgttgct tcgaaacgag cggcccgatt tgagtgtcct
     8281 cgctcgaagg atcgccgacc acaattttgc gcgtggcttg aacgaataat tccacaaatt
     8341 tttcatacac ggaccgctcg acaaaaatgc ggctgcgcgc gcagcaatcc tggccggtgt
     8401 tggcaaagac actcatgggc gaagtggctg cggctttttc caaatcgcaa tcggcaaaaa
     8461 tgatgttggg cgacttgccg ccgagttcca acgagacgcg tttgatgtcc cgcgccgcca
     8521 actgcatgat gtgcgagccc actgatgtcg agccggtgaa tgaaattttg cgaatcaacg
     8581 gatgttttac cagcgcatcg cccatctgct ctcccgatcc ggccaatacc tggagcgcgc
     8641 ccggcggcaa ac
//
LOCUS       DASZMM010000074         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4967816, whole genome shotgun sequence.
ACCESSION   DASZMM010000074 DASZMM010000000
VERSION     DASZMM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4967816"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VG754_01910"
     CDS             complement(<1..1233)
                     /locus_tag="VG754_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HWF17989.1"
                     /translation="MGMKSANVRFLMCPPDLYEVDYVINPWMEGNIHKASREQAVSQW
                     QGLYSLLSQHAEVERITPQAGLPDMVFTANAGVVVGDKFVLSRFLHRERQGEEAPFKK
                     WFKKQGFEVFELPPDLPFEGAGDALMDRAGACLWAGYGFRSELDSHPYLARWLNIEVV
                     SLRLIDPRFYHLDTCFCPLEGGWLLYYPQAFDAYSNRLIEQRIPLEKRLPVGEVDAAR
                     FACNTVNLGQKLFVNQAGGELRNRIEAAGFEVIETPLGEFMKAGGAAKCLTLRLTEPA
                     PARAKAASSVQSRELKLEGHLLDSGLLDRALELTVQSGGSFQILHFALGKQRQSTSNA
                     DIKISAPSDEVLDKIMGQMIELGAVTGPKQENDAELKPVPQAGVAPDEFMATTIYPTE
                     VRVGGKWLRVQDQRMDGVI"
     gene            1467..2525
                     /locus_tag="VG754_01915"
     CDS             1467..2525
                     /locus_tag="VG754_01915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWF17990.1"
                     /translation="MRTAKQTKSLISANLKKFAGECLLNCCDRFPAAKPFLRQWGLRT
                     QPSWFADSVVRVQTPEGRSFQLGSIAQNYLSFELFWRGVSYYEPVTAMLACEMVRTAD
                     TFIDVGANIGFFSLVLSTFKPGLRVISFEPNPKNFQLLQKNVRLNHFDRVTCEPLALS
                     DISGTAALFLSASDMSASLESDFEATPGPSLKVSTVTLDGYLAQRPLSGRLLIKVDVE
                     GHEAAFFKGAQRTISSFKPDIISEVTLRRDAVPLALLKELGYSFYQITDHGLLPTEEL
                     AMVIRGQFRFLNCLLSARPASEVAELFNRIQPRVRRIDLTQTSKFVPAEMLQRFQGAT
                     TAPPAQKPGPAKFLEPAS"
     gene            2622..3677
                     /locus_tag="VG754_01920"
     CDS             2622..3677
                     /locus_tag="VG754_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HWF17991.1"
                     /translation="MIDNDAKLEGLLERLRGSPWVALDTEADSLHAYPEKICLVQIST
                     PAGDELIDPLARLNVEPLLQELTRHELIMHGADYDLRLFRKHHQFVPSVIFDTMLASR
                     LLGITQFGLTHLVGQFLGVTLDKGSQKADWAQRPLTPRMEIYARNDTHYLKSLSDSLK
                     VELVAKRRLSWHQESCARLIADCAVHSPVDPDSVWRIKGSHRLGPPALAVLRELWHWR
                     ETEAITANRPPYFILRHETLIDLAIAAVGGRPIEPLVPPKFSDRRRAGLTSATKLGLA
                     MPHEKQPQPVRHAIRRVTDAQKRRVTDLQNRRDAKAKELSIDPTLIASRATLLDLAED
                     WNAHEKELMNWQRELLK"
BASE COUNT          838 a         1151 c          959 g          836 t
ORIGIN      
        1 gatgacgccg tccatgcgct ggtcctggac gcgcagccat ttgccgccga cgcgcacttc
       61 ggtcggataa atcgtcgtcg ccatgaattc gtcgggcgcg acgcccgctt gcgggaccgg
      121 cttgagttcg gcgtcgtttt cctgtttcgg cccggtgacc gccccgagtt caatcatctg
      181 ccccatgatt ttgtccagca cttcatccga cggcgcggag attttgatgt ctgcgtttga
      241 tgtgctttgg cgctgtttgc caagcgcgaa atgtagtatc tgaaaactgc cgccgctttg
      301 caccgtcaat tccaaagcgc ggtcgagcaa tcccgaatca agcagatgac cttccagttt
      361 caactcgcgg ctttgcaccg aactggcggc cttggcgcgc gcgggtgcag gctcggtcaa
      421 gcgcagcgta aggcacttcg ccgcgccgcc cgctttcatg aattcgccca acggagtttc
      481 gatgacttcg aatccggccg cttcaattcg gttgcgcaat tcaccgccgg cctggttcac
      541 gaaaagtttt tggccgagat tcaccgtgtt gcaggcaaac cgcgcggcgt ccacttcgcc
      601 gacgggcaat cgtttttcca gcgggattcg ttgctcgatc agccggttcg aataggcatc
      661 aaatgcctgc ggataataaa gcagccagcc gccttccagc ggacaaaaac aagtatcgag
      721 atggtaaaag cgcggatcaa tcaatcgcaa cgaaaccacc tcgatgttca gccagcgcgc
      781 caagtacgga tgggaatcca attcgctgcg aaatccgtag cctgcccaca aacaagcgcc
      841 cgccctgtcc atcaacgcgt cccctgctcc ttcaaacggc aggtccggcg gcaattcaaa
      901 gacttcgaaa ccttgtttct tgaaccattt cttgaacggc gcttcttcac cctgccgttc
      961 gcgatgcaaa aaacggctca acacaaattt gtcgcccacc accacgccgg cattggcagt
     1021 gaaaaccatg tccggcaaac cggcttgcgg cgtgatgcgt tcgacttccg cgtgctgcga
     1081 caacaacgaa taaagtccct gccattgact caccgcctgc tcgcgcgaag ccttgtggat
     1141 gttgccctcc atccagggat tgatcacata atccacctcg tacaaatcgg gtggacacat
     1201 caaaaagcgc acgttcgcgg atttcatacc cataggttaa atcacccatt tgaatgccgc
     1261 aatgcaccaa tctgtgttca caggttttgt cacaatcaaa cgcaaaattg tccgcaaaat
     1321 tgaacgaatc gcgcagtttt cccgggcaat cgcaaatcga aaatcgcaaa ccgtaaatca
     1381 taagtggggc agtcacccca ttgttgctcg atttactttc gcgtatgcgt ggtgctcgca
     1441 acgctaagga agtggggata atatatatgc gcaccgcaaa acaaacgaaa agtctcatat
     1501 cagcgaacct caaaaagttc gccggtgaat gtctgctcaa ctgctgcgac cgttttccag
     1561 ccgcgaagcc tttcctgcgc caatggggat tgcgcacgca accgtcctgg ttcgccgatt
     1621 cggtcgtccg cgtacagacg ccggaaggcc gaagtttcca actcggcagc atcgcgcaaa
     1681 attatctcag ctttgaactg ttttggcgcg gcgtcagtta ctatgaaccc gtcaccgcca
     1741 tgctcgcctg cgaaatggtc cggaccgcgg acacgttcat tgatgtgggc gcgaacatcg
     1801 gctttttttc gcttgttctt tcgactttca aaccggggct gcgcgtcatc tccttcgagc
     1861 caaatcccaa aaattttcaa ttgctccaaa aaaacgttcg gctcaaccat ttcgaccggg
     1921 tcacctgcga acctttggcg ttgtccgaca tcagcggcac agcggcactt ttcctcagcg
     1981 cgtcggacat gagcgcctcg ctggaaagtg atttcgaagc aacgcccggg ccttcgctca
     2041 aagtttccac cgtgacgctc gacggctacc ttgcgcaacg gccattgtcg ggccgcctgc
     2101 tgatcaaggt ggatgtcgaa ggccacgaag ccgcattttt taaaggcgcg caacgcacca
     2161 tttcatcctt caaacccgac atcatctccg aagtcacgct tcgccgcgac gctgttccgc
     2221 tcgcgttgtt gaaggaactt ggctacagct tttatcagat caccgaccat gggctcttgc
     2281 cgacggaaga attggcgatg gtgatccgcg ggcagttccg ctttttgaat tgcctgttgt
     2341 cggcccgtcc cgcaagcgaa gtggctgaac tgttcaaccg catccagcct cgcgttcgcc
     2401 gcattgacct cacccaaacc agcaaattcg tgccggctga aatgttgcag cgcttccagg
     2461 gcgcaaccac cgctccgcca gcgcaaaaac cggggcctgc caaattcctt gagccggcaa
     2521 gttgaatttc cgcctgcgga ggtcgtacgt ggtgcgtttg atttttgcgt cgtccgcaaa
     2581 tttcgctttg aactttttgc tttgaattta catttagagc agtgattgac aacgatgcga
     2641 aattggaagg cttgctggag cggttgcgcg gcagcccatg ggtcgcgctc gatacggaag
     2701 cggacagcct tcacgcctat cccgaaaaaa tttgcctcgt ccaaatcagc actcccgccg
     2761 gggacgaatt gatcgatccc ctcgcgcggc tgaacgtcga gccgctcttg caggaattga
     2821 cccggcacga actcatcatg catggcgcgg attacgattt gcgccttttc cgcaagcacc
     2881 accagttcgt tcccagcgtc atcttcgaca ccatgctcgc gagccggttg ttgggcatca
     2941 cccaattcgg cctcacgcat ctggttgggc aattccttgg cgtgacgctc gataaaggtt
     3001 cgcaaaaagc cgattgggcc caacgtccgt tgacgccccg catggaaatt tacgcgcgca
     3061 acgacacgca ctatctcaag tcgctctcgg attctttgaa agttgaactt gtggcgaaga
     3121 gacgtttaag ctggcaccag gaatcgtgcg cccggctcat cgccgattgc gcggtgcatt
     3181 ctccggttga tcccgactcg gtttggcgca tcaaaggcag tcatcgcctt ggcccgcctg
     3241 cgctggccgt cctgcgggaa ctttggcatt ggcgcgaaac cgaggccatc accgccaatc
     3301 gcccgcccta tttcattttg cgccacgaaa cgctgattga ccttgccatc gcggcagtcg
     3361 gcggtcggcc gattgaaccg ctcgttccgc caaagttttc cgatcgccgc cgcgctggtt
     3421 taacatcggc gaccaaactc ggtctcgcca tgccgcacga aaaacagccg caaccggtgc
     3481 gccatgccat ccgccgcgtg accgacgccc agaaacgccg cgtcaccgat ttgcaaaatc
     3541 gccgcgacgc caaagccaag gaactcagca ttgacccaac gctaatcgcc agccgcgcca
     3601 ccctgctcga cctcgccgaa gattggaacg cacacgaaaa agaattgatg aactggcaga
     3661 gggagttgtt aaagtaggga accgaatgaa ctttcctttt ctcaacaaga agaaaaaaca
     3721 cttaaaatac aacagcgtag ttggttggct tcatcgcgaa tccgtgccaa aagaattttt
     3781 atca
//
LOCUS       DASZMM010000075         5647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_845283, whole genome shotgun sequence.
ACCESSION   DASZMM010000075 DASZMM010000000
VERSION     DASZMM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845283"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(446..1285)
                     /locus_tag="VG754_01925"
     CDS             complement(446..1285)
                     /locus_tag="VG754_01925"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HWF17992.1"
                     /translation="MKTKIEPDSPGAARTTVLMLLAIGIAAILSGIGLVYSKHLSWQQ
                     KEPSPVAEVHGEPIQAVAVPATNPVSDITTNTPTKRRHPKPPSVQRRMLLAQLAQTGA
                     VSNGDTSLPSGTLGNAADMAKTNLVSASSLPPVQERIPGYAEINFSTLSGFDFVLDKE
                     MADGSTAPAQMLAKAKAEIPANVLALDGAKSVIQGFLLPVKMDEGLTVEFLLMRSQSM
                     CCYGVAPKINEWITVQMTGKGVKPTMDRPIAVVGVLHVAPMEENGFLTGIYRLDGERV
                     ISP"
     gene            complement(1388..2101)
                     /locus_tag="VG754_01930"
     CDS             complement(1388..2101)
                     /locus_tag="VG754_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWF17993.1"
                     /translation="MQNKISRRFFAGIVVGALLLLQVAGSISLLAQEQPYIQPGHPDG
                     IALLPPPPLNGSKEAAADLLEARAVVKGRTPEEEAHAKKFASLSFSLFEPAIGPIFQP
                     GKLPKTEALMKKVRAEIGEAINIPKDHWKRMRPYQVDPELASGMTEKSYSYPSGHSTR
                     GTVYSLILAEIFPEKKDAILQVGRDIGWDRVVIAMHFPTDVYGGRVLGKAIVRELMAN
                     PAFQHDLEAAKAEAQAAKP"
     gene            complement(2204..2761)
                     /locus_tag="VG754_01935"
     CDS             complement(2204..2761)
                     /locus_tag="VG754_01935"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HWF17994.1"
                     /translation="MLAQSSAKPATPSAIRGEAIQPAAAPGTNAAVKAPSPAPNVPAT
                     AGGYESIGFEKLASYEYTAPDTQVTNLPPEGDVANKCIPDNIKKMDGKLVQVRGFMLP
                     LKIDAGYVTEFLIMRNQSACCFGTAPKLTEIVDIKLTGKGVDAIMDQPVTVKGRLHVG
                     TVREDGYITCIYKMDGEKLIENPGN"
     gene            complement(2857..3498)
                     /locus_tag="VG754_01940"
     CDS             complement(2857..3498)
                     /locus_tag="VG754_01940"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HWF17995.1"
                     /translation="MKNRNEFTLRAAKIAARILTGAGLLALLVSPPPHLWGQQAAIHG
                     EPIGSPSANTNAPASTNAPPAKPPEEKAKGSEVGFDKLAGFKMNTTDDMLMGKGDKLT
                     ISQEVISQIPASVKAFNEKLVSVTGFMLPLEQDHGKVTEFLLLRNQSACCYGATPQIN
                     EYVIVRLAGRGIEAQMDRLITATGTLHVGEIRENKFLSGIYRMEADKVAISGQ"
     gene            complement(3482..3955)
                     /locus_tag="VG754_01945"
     CDS             complement(3482..3955)
                     /locus_tag="VG754_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF17996.1"
                     /translation="MLLAFACGCLTGCGDSNPTKPPSTVAKHEHKPPHGGTPVVLGDE
                     EYHVELVLDAPAGKLDAYVFDGELENFVRSTAPEFQVLAKLPSGGQTLVFKPIPNSAT
                     GETVGDTSLFETQADWLKKETNFDAVLKQITVRGKSYENVEFNFPKGNDKDEKPK"
     gene            complement(4016..5227)
                     /locus_tag="VG754_01950"
     CDS             complement(4016..5227)
                     /locus_tag="VG754_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWF17997.1"
                     /translation="MTLPLIVFRSLRQHALSTIVTALSIALGGGLLMSVWVVKDQSRA
                     IFTGVNGGFDAVLGARGAKLQLVLNAIFHLEASPGNLGWSDYQDIKNNPAVQLAIPIA
                     VGDNYLGYRLVGTLTELFDKVEYAPGKHYELEPGGEMFDATRREAVVGSFVAQKLGLK
                     RGDTFHPFHGLIYNEKNQHSETYVVVGVLKPSNTPADRVIWIPLEGIQKMTGHDPNAA
                     TDVSAVLVKLKAGSAASGFRLDMMYNKQGNRLTFAWPIGQVTAELFNKIGWFDRLLEL
                     VSYLVALVATGSILASIYNSMNERRREIAILRALGARRRTIFGAILLEAASISALGMV
                     IGFGIYIVIMGGAAAVIRTQTGVVLEPAQYNPVMFWAPLALILLGALAGIVPAMKAYR
                     TDVAAHLMPTS"
     gene            complement(5224..>5647)
                     /locus_tag="VG754_01955"
     CDS             complement(5224..>5647)
                     /locus_tag="VG754_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF17998.1"
                     /translation="GYIFQTFNLLQGYTCLENVLLGMSFGPGADKAFAQALLERVGLG
                     NRLKHYPRQLSTGQQQRVAVARALANRPKLVLADEPTGNLDHRNAGASLDLIRAACGE
                     NGAALLLVSHDREVLGKFEQVHSLEKINRAAAGAEVTV"
BASE COUNT         1202 a         1559 c         1552 g         1334 t
ORIGIN      
        1 actggttttt gtcagcttgt tagtttgttc ttcaggatgc ttttgcatcc ctccttattt
       61 aaacacatgt acacaaaagt acagtgccaa tttgtcacac cctagctttt gtttactctc
      121 caatttatat gcgtctgccc tgggattcaa cgtaattgct gaaatagtta gtggacacac
      181 ctcatttttc tgcatgctcc ggaccctttt cggtttgtat ttgcgggtta gcacacgtcg
      241 cggtcccctc accccagccc tctccctaaa accggaacga tgcagttgag ccacggattg
      301 aacactgatg aaacacggat gggaggtgag aacagctatt ttcgacattc taaattcatt
      361 atgacgaacc ttgttctaat ttccaattcc actgaaaaaa agcgatgctt aaaaaactga
      421 aaaactcacg ttttcagcta cacggttagg ggctgatgac ccgctcgccg tccagcctgt
      481 aaatgccggt gagaaagccg ttctcctcca tgggcgcgac gtgcagcacg cccaccacgg
      541 caatgggccg gtccattgtg ggcttcacgc ctttgccggt catctgtacg gtgatccatt
      601 cgttgatctt gggcgcgaca ccgtaacagc acatggattg actgcgcatc agcaagaatt
      661 ccacggtcag cccttcatcc attttcactg gcaagagaaa accttgtatc actgatttcg
      721 cgccgtccaa ggccaggaca ttcgcgggaa tttctgcctt cgcttttgcc agcatttgcg
      781 ccggagccgt gctgccgtcg gccatttcct tgtccaaaac aaaatcgaaa ccggacaacg
      841 tgctgaaatt gatttccgca taaccgggaa tccgctcttg aacgggcggg agacttgagg
      901 cggaaacgag attggtcttg gccatgtccg cggcatttcc cagcgtgccg gacggcagac
      961 ttgtgtcacc gttgctcacc gcgccagttt gcgcgagttg ggcgagcaac atgcggcgtt
     1021 gcacggaagg cggcttggga tgacgccgct tcgtcggagt attggtggtg atgtcggaaa
     1081 ctggatttgt ggcagggacc gcaacggctt ggattggttc accgtgaact tccgcgaccg
     1141 gagagggttc tttctgttgc catgaaagat gctttgaata aaccaaacca atgcccgaaa
     1201 ggattgccgc aattccgatc gccagcaaca tcagcaccgt cgttcgcgcc gcgccgggtg
     1261 aatctggttc aattttcgtc ttcaaagcca attgaattca acataacgca agaggctgaa
     1321 aactcacgtt ttcagctaca cacgatgcaa tatctgaaaa ctcacgtttt cagctacgaa
     1381 gagcgcatca aggcttcgcc gcctgcgctt cggcttttgc ggcttcgagg tcatgttgaa
     1441 atgccgggtt ggccatcaac tcgcgcacga tcgccttgcc caaaacccgg cctccataga
     1501 catccgtcgg aaaatgcatg gcgatcacca cccggtccca accgatgtcc cgtccgactt
     1561 gaagaatggc gtcctttttc tcaggaaaaa tttcggccag aatcagcgaa taaaccgtcc
     1621 cgcgcgtcga atggccgctc ggataactat aactcttttc agtcatgccc gatgcaagtt
     1681 ccgggtccac ctgataggga cgcatccgtt tccaatggtc tttggggatg ttgatcgctt
     1741 cgccgatttc cgccctcact tttttcatca acgcctcggt cttgggcagt ttgccgggtt
     1801 gaaaaatcgg gccgatggcc ggctcgaaca acgaaaatga aaggctcgcg aatttcttgg
     1861 cgtgggcttc ttcctcgggt gtgcgtcctt tgaccaccgc gcgggcttcc agcaaatccg
     1921 cagccgcttc tttcgagccg ttcaatggcg gcggcggaag caacgcaatt ccatccggat
     1981 gccccggctg aatataaggt tgctcctgcg ccagcagtga aatggaccct gccacttgca
     2041 acaacaagag cgcgcccacc acgatgccgg caaaaaagcg ccgggatatt ttattttgca
     2101 tggggaaacg gtatttcgag ttcaaagagt tttcgcgatc aattttcatc ggcttgtttt
     2161 accaaagccg gggcgattgc caaaataaat gtggcgcgtg cttttaattg ccggggttct
     2221 caatcaattt ttcgccatcc atcttgtaga tacaggtgat gtagccatcc tcacgcaccg
     2281 tgccgacgtg cagcctgcct ttgacggtga ccggttggtc catgatggca tcaacgccct
     2341 tgccggtcag tttgatgtcc acgatttccg tgagcttggg cgctgtgccg aagcaacaag
     2401 ccgattggtt gcgcatgatg agaaattccg tcacgtaacc ggcgtcaatc ttcaatggca
     2461 gcatgaatcc cctgacctgg acgagcttgc catccatttt tttgatgttg tcgggaatgc
     2521 acttgttcgc cacgtcgccc tccggcggca aattggtgac ctgcgtgtcc ggcgcggtgt
     2581 attcgtagga agcaagcttt tcgaaaccaa tgctttcgta tccaccggca gttgccggaa
     2641 cattgggcgc aggtgaaggg gcttttactg cggcattcgt tcccggcgcg gcggcgggtt
     2701 ggattgcctc accgcgaatt gcggaaggcg tggccggttt tgccgaggat tgggcgagca
     2761 tatttgcgcg cggcgcaaac aaagcaagga aagcaatcgc gaccggaacg cattggcgga
     2821 atttaagcat catgattttt cctttctcat ttgcgatcat tgtcctgaaa ttgcgacctt
     2881 gtcggcctcc atccggtaaa tgccgctgag gaatttgttc tcgcgtattt cgccgacgtg
     2941 cagcgtgccg gtcgcggtga tcaagcggtc catctgcgct tcgatgccgc gccccgcgag
     3001 ccggacgatg acatactcat tgatttgcgg agtggccccg tagcaacagg ccgattgatt
     3061 gcggagcaga agaaattcgg tgacttttcc atggtcctgc tccaagggaa gcatgaagcc
     3121 ggtgacggac accaattttt cattgaaggc tttcactgaa gccggaattt gcgagatgac
     3181 ttcctgcgaa attgtgagct tatcgccttt gcccatcaac atgtcgtccg tcgtgttcat
     3241 cttgaatccg gcaagcttgt cgaaaccgac ttccgaacct tttgcctttt cctcaggcgg
     3301 tttggccggc ggagcgttgg tgctcgcggg cgcgttcgtg ttggctgaag ggctgccgat
     3361 cggttcgcca tgaattgcgg cttgctgtcc ccaaagatga ggcggcggcg agacgagcaa
     3421 cgccagcaag cccgcgcccg tcaggattcg cgcggcgatt tttgcggcgc gcaacgtaaa
     3481 ttcatttcgg tttttcatcc ttgtcatttc ctttcgggaa gttgaattcc acattttcgt
     3541 aagatttgcc tctgaccgtg atctgcttca gcacggcgtc gaagttcgtt tcttttttta
     3601 gccagtccgc ctgggtttca aaaagcgaag tgtcgccgac ggtttcgccg gtggcgctgt
     3661 tgggaatcgg tttgaaaacc agcgtttgcc cgccggacgg caatttcgca agcacttgaa
     3721 attcaggcgc ggtgctgcgc acgaaatttt ccaattcgcc gtcgaaaaca tacgcgtcga
     3781 gttttccggc aggggcgtcc agcaccagtt cgacgtgata ttcttcgtcg cccaaaacga
     3841 ccggcgtgcc gccgtggggc ggtttgtgtt cgtgtttggc gacggtggag ggtggttttg
     3901 tggggttgct gtcgccgcaa ccggtcaggc atccgcaagc aaaagcgagg agcaaaattg
     3961 catttaacgc ggctaatttc atggcgtgca agttattcaa gtaatctcaa aataatcagg
     4021 aggttggcat cagatgcgcg gccacgtctg tgcggtacgc tttcatcgcg ggtactattc
     4081 ccgccagcgc gccgagcaat atcaaggcga gcggcgccca aaacatcacg ggattgtatt
     4141 gcgcgggttc cagcaccacg ccggtttggg tgcgaatcac cgccgccgcg ccgcccatga
     4201 tgacgatgta aatcccaaaa ccgatcacca tccccagcgc cgaaatggat gcggcctcca
     4261 aaaggattgc gccgaaaatt gtcctgcgcc gcgcgcccaa ggcccgcaga atggcaatct
     4321 cgcgtcgccg ctcgttcatc gaattataaa tgctcgcgag gatcgaaccg gtcgccacca
     4381 gcgcgacgag atatgaaacc agttccagca aacggtcgaa ccagccgatt ttgttgaaca
     4441 attccgccgt cacctgaccg atgggccatg cgaatgtcag gcggtttcct tgtttgttgt
     4501 acatcatgtc cagccgaaaa ccggaggccg cgctgccagc cttcaatttc accagcacgg
     4561 cgctgacatc ggtcgcggca ttcggatcgt gaccagtcat tttttggatg ccttccagcg
     4621 ggatccagat gacgcgatca gcgggcgtgt tggatggttt caacacaccg accaccacgt
     4681 acgtttccga gtgctgattt ttctcattgt aaatcaggcc gtggaaggga tggaacgtgt
     4741 cgccgcgttt gaggccaagt ttttgcgcga cgaaactgcc gaccaccgcc tcgcggcgcg
     4801 tggcatcaaa catttcgcca ccgggctcca attcataatg tttgccgggc gcgtattcca
     4861 ccttgtcaaa aagctccgtc aatgtgccga cgagccggta gccaagataa ttgtcgccca
     4921 cggcaatcgg aatggcgagt tgcacggcgg gattgttctt gatgtcctgg taatcggacc
     4981 agccaagatt tcccggcgaa gcttcgagat gaaaaattgc gttgagcacg agttgcaatt
     5041 tcgcgccgcg cgcgccgagc acggcatcga atccgccatt gacgccggtg aaaatcgcgc
     5101 gcgattggtc cttcaccacc cagacggaca tcaacaaacc gccgcccaac gcgatggaaa
     5161 gcgcggtcac aatggtcgaa agcgcgtgtt ggcgcaggct gcgaaataca atgagaggaa
     5221 gcgtcatacg gtcacctccg cgccagcggc ggcgcgattg attttctcca acgaatgcac
     5281 ctgctcaaat ttgccgagca cttcgcggtc gtggctcacc agcagcagcg ccgcgccgtt
     5341 ttcgccgcaa gcggcgcgga tcaaatcgag cgacgcgcct gcattgcgat ggtcgaggtt
     5401 gccggtcggt tcgtcggcaa ggacaagctt ggggcggttc gcaagcgcac gagcgacggc
     5461 gacgcgttgt tgctggccgg ttgaaagctg gcgcggataa tgtttgaggc ggttgccgag
     5521 accgacacgt tccagcaacg cttgggcgaa tgccttgtcc gcgcccggtc caaacgacat
     5581 gccgagcagc acattttcaa ggcaggtata gccttggagc agattgaatg tctgaaaaat
     5641 gtagccg
//
LOCUS       DASZMM010000076         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4421995, whole genome shotgun sequence.
ACCESSION   DASZMM010000076 DASZMM010000000
VERSION     DASZMM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4421995"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..>1530)
                     /gene="glyA"
                     /locus_tag="VG754_01960"
     CDS             complement(275..>1530)
                     /gene="glyA"
                     /locus_tag="VG754_01960"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWF17999.1"
                     /translation="NIASSNELKLKAVDPEIAMAICHERQRQQENIELIASENFTSPA
                     VMEAQGSVLTNKYAEGYPKKRWYGGCENVDTVEQLAIARARKLFNAEHANVQPHSGSS
                     ANMAVYFAFLKPGDKMLTMDLTHGGHLTHGNKANFSGKFFEIVHYGVRKEDELIDYDQ
                     LAKVAREQRPKMITVGASAYPRIIDFARMGEIAKEVGALLLADIAHIAGLVVAGLHPS
                     PIEHADFVTTTTHKTLRGPRGGLVMCKERYAKEIDSQAFPGIQGGPLMHVIAAKAVCF
                     QEALQPSFKTYQQQIIKNAKALAEGMRRNSYRLVSGGTDNHLMLVDVGARGLTGKDCQ
                     IVLDEAGITVNKNTIPFETRSPFQASGIRLGTPAVTTRGMKEQDMAAIADMISEVLMD
                     IKNLDTVAKVRQRVRELTARFPLPY"
BASE COUNT          302 a          411 c          434 g          383 t
ORIGIN      
        1 gccgtcgcca ttttcaacgt cgggtggaag atcgccgggt ttgagcgcct tggctttgcc
       61 ggcgcctttg ggttttaagg ccttctttgt actgactctg ggcatattaa gtaataacta
      121 gactggttaa attgtttcgt cgcgaatgga ggacaatcat gcggtcactt tgcccttcgc
      181 gacagcgggt tggacgaccc gaaacttgtt taatttaata attcaaggga cgcgggtacc
      241 aaccggggca caccgaaaga ttcgattaca aatctcaata cggcaaggga aatcttgcag
      301 ttaattcacg cacacgttgg cgcactttgg cgacggtgtc aaggttcttg atgtccatca
      361 gcacctcgct gatcatgtcc gcgatggcag ccatgtcctg ttccttcatc ccgcgcgttg
      421 taacagcagg cgtgccgagg cggatgccgc tggcttgaaa cggcgagcgc gtctcaaagg
      481 gaatggtgtt cttgttcacg gtgatgcctg cttcatcgag cacgatctgg caatccttgc
      541 cggtcaatcc gcgcgcgccg acatccacca gcatcaggtg attgtccgtc ccgccgctga
      601 ccaaccgata gctgttgcgc cgcattcctt cagccagcgc cttggcgttt ttgatgatct
      661 gctgctgata agttttgaaa gaaggttgca gcgcttcctg aaaacaaacg gccttagccg
      721 cgatgacatg catcaacggc ccgccttgaa tgccaggaaa cgcctgcgag tcaatttcct
      781 tcgcataccg ttccttgcac atgaccaatc ccccgcgcgg cccgcgcagc gtcttgtgcg
      841 tggtggtggt gacaaaatcg gcgtgttcaa tcgggcttgg atgcaatcca gccacaacca
      901 acccggcgat atgcgcgatg tccgcaagca acaaagcgcc gacttccttc gcgatttctc
      961 ccatgcgggc gaaatcgatg atgcgcggat aagcgcttgc gccgacagtg atcatcttgg
     1021 gccgctgttc gcgggcgacc ttggcgagtt gatcgtaatc gatcagttca tcttccttgc
     1081 ggacgccgta atggacgatc tcaaaaaatt tgccggaaaa attggccttg ttgccatggg
     1141 tcaaatggcc gccgtgggta aggtccatcg tcagcatctt gtcgccgggc ttgagaaagg
     1201 cgaaatagac ggccatgttc gcgctgctgc cggagtgggg ctgcacgttg gcgtgttcgg
     1261 cattgaacaa tttgcgcgcg cgggcgatgg cgagttgctc gacggtgtcc acgttttcgc
     1321 aaccgccata ccaacgtttt ttgggataac cttccgcgta tttgttggtc agcaccgagc
     1381 cttgcgcctc catgaccgcc ggactggtga aattttcact ggcgatcagt tcgatgtttt
     1441 cctgttgccg ctggcgttcg tggcagattg ccatggcgat ttcagggtca acggctttga
     1501 gtttgagttc gtttgaggag gcgatgtttt
//
LOCUS       DASZMM010000077         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2503651, whole genome shotgun sequence.
ACCESSION   DASZMM010000077 DASZMM010000000
VERSION     DASZMM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503651"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VG754_01965"
     CDS             <1..1242
                     /locus_tag="VG754_01965"
                     /inference="COORDINATES: protein motif:HMM:NF037003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18000.1"
                     /translation="DSYWDNMSGTQLQYFANLCYANGQNPGIYWSPFVWWGAASNAAT
                     SHVEGSTYSYDQVALKTTNGSYQVIDGALALDPTHPGTKQRVDYYINMFKGYGFDYLK
                     LDFLTHGALEGIHYDTNVTTGIQAYNQGMQYVLNDMGTNMYCSESIAPIFPYQYGHSR
                     RIACDTSTYISETSYEMNSVTYGWWLSGRLYQYNDPDCMKFSGATTNENQSRLISCAV
                     SGTVFLDSDDLTSTTAQALAETCLTNARINAVAQAGRSFTPVEGNTGNNAATLFVRQD
                     GATWCVAVFNYSSNAITTNINLARAGIAGTYVPVDLWSGAISTITNSTLNVNLNARQS
                     KLFRLFTIPMLIFATVKSGNAVSFKLQGDAGFNYAIQKTTNLVQWNTVATLTNTTGQA
                     QFTETNLPVSGNGFYRAMLIP"
     gene            1353..3746
                     /locus_tag="VG754_01970"
     CDS             1353..3746
                     /locus_tag="VG754_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18001.1"
                     /translation="MKKRRKTKSSGPRHPPPTSPKLAGWKLWRLRIFSAFLIPVLVLV
                     LAELVLRLAGFGYPTAFLLPSVNQGKKTFVQNNEFGWRFFGKRKSRTPSAISILQKKP
                     PGAIRIFVFGESAAFGDPQPAFGLPRVLQATLSLRHPEYKFEVVNVAMTAINSHVILP
                     IARDCARANGDIWVIYMGNNEVVGPFGAGTVFGTQSLPVPLVRANLALKTTRLGQLLD
                     AVREHWNKPPPDKSEWGGMLMFLDQQVREDDPRLADVYRNFETNLKDIIRAGQKSGAG
                     IVASTVAVNLKDCSPFASQHRAGLMESDKQKWESLAREGINAQTSGQFLDAANKFDAA
                     AQLDDSDAELEFRMGKCLLASGNISGAQKAFSKARDLDTLRFRCDSRLNELIRKTATG
                     RESEKVLLADPEQSFAAASKDGLPGWNYFYEHVHLTFEGNCLLARTLAEQVEKLLPRE
                     SSAIDHLWPTDAECAGRLGRTDRDLKPAVADVLSRLTDPPFVTQINHQEQIQYLGEQL
                     RKANSTLTNALQATIAALNAAPDDAQLYQQLAALQEEAGDYKQAESAARRATELLPSS
                     SENWSQLGHVLVQQKNYAEAADAYRRAFDLNPQDAAPLQNVAMALVKLGRNDEAKKEF
                     KHVLEITPRFGLAWLGLGQLLEGEGHKEEATNCYQKALQNRIHKAPELTMLARFCMTR
                     GWLEAACTNYDEAAKLDSFNSALASETGQAHFLYGMELGKAGQTAAAEREFRESVRLM
                     PEVVEARLNLGIALYREQKWNESLNEFQAVAARSPTNELARQYLELLHGKTGHSERQ"
BASE COUNT          934 a         1061 c         1031 g          862 t
ORIGIN      
        1 gattcctatt gggacaacat gagcggcacg caacttcaat acttcgcgaa tctctgttat
       61 gcgaacggcc agaacccggg catctattgg tcgccgtttg tctggtgggg agcggcaagc
      121 aacgccgcca caagccacgt cgaaggttcc acttacagtt acgaccaggt cgcgctcaag
      181 accaccaacg gcagctatca ggtgattgat ggcgcgcttg ccttggaccc aacgcatccg
      241 ggcaccaaac aacgggttga ttattacatc aacatgttca agggatatgg tttcgattat
      301 ctcaagctcg atttcctcac tcacggcgca ttggaaggga tccactacga caccaatgtt
      361 accaccggca tccaggctta caatcagggc atgcaatatg tgttgaacga catgggcaca
      421 aacatgtatt gcagcgaatc catcgcgccg atctttcctt atcaatacgg gcattcccgt
      481 cgcatcgcgt gcgacacctc cacttacatc agcgagactt catacgaaat gaattcagtg
      541 acgtacggtt ggtggctgag cggacggttg tatcaataca acgacccgga ttgcatgaaa
      601 ttctccggcg cgaccaccaa tgagaaccag agccgtctca tcagttgcgc cgtttccggc
      661 acggtttttc ttgacagcga cgacttgact tccacgacgg cccaggcgct tgctgaaact
      721 tgcctgacca acgcccgcat caatgcggtc gcgcaagctg gccgaagctt caccccggtg
      781 gaaggcaaca ccggcaacaa tgccgcgact ctgttcgtcc ggcaggatgg cgccacatgg
      841 tgcgtggccg tgttcaatta cagttccaac gccatcacca caaacatcaa cctggcacgc
      901 gcgggcatcg cgggaactta cgtcccggtg gatttgtgga gcggcgcgat atcgaccatc
      961 accaattcaa cattgaacgt gaacctgaat gccagacaat ccaagctgtt ccgattgttc
     1021 acaatcccca tgttgatttt tgccactgtt aaatccggca acgcggtgag cttcaagttg
     1081 caaggagatg ccggcttcaa ctacgccatc caaaaaacaa ccaatctggt ccaatggaac
     1141 acagtggcaa ccttgacgaa caccaccggc caggcgcaat tcaccgaaac caacctgccc
     1201 gtttccggaa acggttttta ccgcgcaatg ttgattccgt aaacagcacc ggcacaggtt
     1261 gagaaggatt tcggcttcat cagcatgttc gatagttcga taatgtcagc ttgacaagcg
     1321 cagccatatt ttgaaatttg tggctgccac caatgaaaaa gcgccgcaag accaaatcaa
     1381 gcggtccacg ccaccctcct ccaacctcgc caaaactcgc cggttggaaa ttgtggcggc
     1441 tgcggatttt ctcggctttt ttgattccgg ttcttgtgct ggttctggcc gaactggttt
     1501 tgcgtcttgc cggatttgga tacccaaccg catttctgct tccttccgtc aatcaaggca
     1561 aaaaaacttt cgtgcagaac aacgaatttg gctggcgatt ttttgggaaa cgcaagtcgc
     1621 gcacgccgtc cgcaatttcc atccttcaaa aaaagccgcc cggcgccatc cgaatttttg
     1681 tgtttggcga atcggctgcg tttggagatc cgcagcctgc ctttggcttg ccgcgtgtgc
     1741 tgcaagccac gttgagtctc cgccatcctg aatacaagtt cgaggtggtc aacgtcgcga
     1801 tgactgccat caattcgcat gtcatcctgc cgatcgcgcg tgattgcgcc cgcgccaatg
     1861 gcgacatctg ggtgatttat atggggaaca atgaagtcgt cggcccgttc ggcgcgggga
     1921 cggttttcgg gacgcaatct ttgcctgttc cgctggtgcg cgccaatctt gctttgaaga
     1981 ccacgcggtt gggccaatta ctggatgcag tccgcgaaca ttggaacaag cctccgccgg
     2041 acaaaagcga atggggcggc atgttgatgt tcctcgatca acaagtgcgg gaggatgatc
     2101 cgcggctggc tgatgtttat cgcaatttcg aaaccaattt aaaggacatc atacgcgccg
     2161 gtcaaaagag cggcgctggc attgtggcaa gcacagtcgc ggtcaacttg aaagattgct
     2221 ccccgttcgc ttctcagcat cgagcggggt tgatggaatc ggacaaacaa aaatgggaaa
     2281 gcctggcacg cgaaggcatc aacgcgcaga cctcgggaca gtttttagac gctgccaaca
     2341 agttcgatgc cgcagcgcag ttggatgatt ccgatgccga attagaattt cgcatgggca
     2401 aatgtttgct tgcatccgga aatatttccg gcgcgcagaa agcgttcagc aaagcccgcg
     2461 atctcgacac gttgcgattt cgttgcgaca gccgtttgaa cgaactcatc aggaagacgg
     2521 cgactggacg tgaaagtgaa aaggttctgt tggcggaccc tgaacaatct ttcgcggccg
     2581 cgtccaaaga tggattgccg ggttggaatt atttttacga acacgtgcat ttgacgtttg
     2641 aaggcaattg cttgttggcg cggactttgg cggaacaggt cgaaaagcta ctgccccggg
     2701 aatcgtctgc tatcgatcat ctgtggccca cggatgcaga atgcgccggg cgattgggcc
     2761 ggacagaccg cgatttgaaa ccggctgtgg ccgacgttct cagccgcttg accgatccgc
     2821 cattcgtcac acaaataaat caccaggaac aaattcaata tctcggcgag caacttcgga
     2881 aagccaattc gactttgacc aacgccttgc aagcgaccat tgccgcgctg aatgcggccc
     2941 ctgatgacgc gcaactttat caacaactcg ccgccttgca ggaagaggcg ggcgattaca
     3001 agcaggccga atcagccgcg cgtcgcgcaa cggaactttt gcccagcagc tccgagaact
     3061 ggtcgcaact cgggcatgtc ctggtgcaac aaaaaaatta tgcggaagcg gcggatgctt
     3121 accggcgggc tttcgacttg aaccctcaag atgccgcgcc gttgcaaaat gttgcgatgg
     3181 ccctggtcaa attgggcagg aatgatgagg caaaaaagga attcaagcac gttctcgaaa
     3241 tcacgccgcg cttcgggctc gcgtggcttg gccttggcca attgctcgaa ggcgaaggcc
     3301 acaaagagga ggcgacgaat tgctatcaga aagcgttgca aaatcgcatc cacaaggcgc
     3361 cggagttgac gatgctggcg cgtttttgca tgacccgcgg atggcttgaa gcagcctgca
     3421 cgaattatga tgaagctgcc aaactcgatt cattcaattc cgctttggcc tcggaaacgg
     3481 ggcaggccca ttttttgtat ggaatggaac tgggaaaagc cggtcaaact gccgcggctg
     3541 aacgtgaatt tcgggaatcc gtgcgcctga tgccggaggt ggtcgaagca cgcttgaatc
     3601 tgggcattgc gctttatcgc gagcagaaat ggaatgaatc gctcaatgaa ttccaggcgg
     3661 tcgccgcgcg aagtcccaca aatgaattgg cccggcagta cctggagcta ctccacggaa
     3721 aaaccggcca ttctgaacgg caataacctt gccgtcggtt catgtggcca ttttatgcca
     3781 cattacgtgc ttctcttttc cgctataatc cttgattcta acatgaaccc cggaatgaag
     3841 ttccagccca tgcgctgtag tctggttgaa cgcagatttg acaaatat
//
LOCUS       DASZMM010000078         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_578066, whole genome shotgun sequence.
ACCESSION   DASZMM010000078 DASZMM010000000
VERSION     DASZMM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578066"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..182
                     /locus_tag="VG754_01975"
                     /pseudo
     CDS             1..182
                     /locus_tag="VG754_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415332.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(224..1114)
                     /locus_tag="VG754_01980"
     CDS             complement(224..1114)
                     /locus_tag="VG754_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWF18002.1"
                     /translation="MELRHLRYFVAVAEEQNVTRAALRLHVSQPPLSRQIRNLEDELG
                     VALFDHDANAVRLTEAGRVFLTEARLILRHAEGAVQMAKEVAGGKRGEIQIGYAPSLT
                     VELLPRALRYFRKSNPGVRVQLHDLSTQEMLRGLRDGKLHAALLVQVPPRTLAGLVFE
                     ELQRLTVCVALHPAHPLARARRVGLEQVAKEHLIAFTLADYPEHQAWIADLFAPLNRS
                     PQIVEEYDSITSLIAAVESGRGLAVIAQLPNGLASSRLKVRPLQPAPPPLGVGIAYRK
                     KFRSTVTNNFIDAAKRAKVD"
     gene            1255..1893
                     /locus_tag="VG754_01985"
     CDS             1255..1893
                     /locus_tag="VG754_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWF18003.1"
                     /translation="MEAKITKNYHERLTPDNATLLLIDHQVGLFTGVRDIPVAELKHN
                     VVALAKAARVLGMPIIVTATSPEMWGPTIPELTEALPGASVIARTTVNAFDDARFAKA
                     VEATGRKKLIIAGVSTEVCLALPAIYATGIGYDTYAVIDASGTFSETKRITGLMRMVQ
                     AGVVVTDYSTVAVEMLKDNASPKAGDLYAALGMPFAGLVGQLAGAFAKSRSA"
     gene            complement(1925..>3915)
                     /gene="feoB"
                     /locus_tag="VG754_01990"
     CDS             complement(1925..>3915)
                     /gene="feoB"
                     /locus_tag="VG754_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HWF18004.1"
                     /translation="LSPQSLDEQISRDVLLHRLKELPAPSLIVVVVDASNLQRNLYYA
                     TQVIELGHPTLLALNMVDVAEANGHEIDAAQLSASLGVPALPIVASSGRGLPQLRQQI
                     PAALSEKPALKTPKQFFELPSDFSREINSLAELLEKHFPEHHRQALAEALLILTNEMA
                     LASSLEHYPAVFQQAVEAARQRLEASGIEWRSAAIEARYACVMAVYQGAVTETRRDEE
                     TFSDKLDRILTHKVWGTLIFVAIMALMFQSIFTFANIPMDALKFVVGRIGGFVGHLIP
                     PGELNSLLVDGVIAGVGAVVVFLPQILLLFLFIGLLEDTGYMARAAFLMDRLMSKVGL
                     HGKSFIPMLSSFACAIPGIMATRTIESPKDRLVTILVAPLMSCSARLPVYTLLIGACI
                     PDKRIFGFFKLAGLTMLTMYLLGIVVALLMAWLFKKTLLKGETPMLIMELPPYKRPLA
                     RVVLRHMWDRSRLFLRRAGTVILGINILLWFLATYPHNPAVKRDFEAKRAAIASTNTA
                     QLAALDNQEQGENLRYSFAGRMGRLIEPAIAPLGFDWKIGIGVISSFAAREVFVSTMS
                     TVYNLGHGEADDKVADLQKVLPDQKRPDGTTLYTPLLAVTLMVFYVFALQCVSTVAIV
                     RRETNSWKWPVFQWLYMGALAWTLAFITYQGGRLLGWH"
BASE COUNT          919 a         1101 c         1096 g          799 t
ORIGIN      
        1 tggcaccgct tggaaatcga aaccgagctg gtacattgtg gccacgagtg accacaccgt
       61 gcagcccgaa cggcaacgcg aatggggtgc gaaaacctac gaggttgaca gcagccacgc
      121 ccccatgctc caacccaaaa ctcgtgatcg acggaatccg caccgctgca aacgcaccat
      181 aatcgcggcc aacaaatgcg cgaaggaact cgtttgagcg ttgtcagtca acctttgctc
      241 gcttggctgc gtcaataaag ttgttcgtta ccgttgagcg aaatttcttg cgatacgcga
      301 tgccgactcc gagtggtggt ggtgctggtt gcaacgggcg aactttcagt cgcgaacttg
      361 cgagaccatt gggtaattgc gcaatcacag cgaggcctct gcccgattct accgccgcga
      421 tgaggctggt gatgctgtcg tattcctcga ctatctgggg cgagcggttc agtggcgcga
      481 acaagtcggc aatccatgcc tgatgctctg gatagtccgc aagcgtgaag gcgatgagat
      541 gctctttggc aacttgttcc agcccgactc tgcgcgcccg cgccagcgga tgcgccggat
      601 gcaaggcaac gcaaaccgtc agacgctgca attcctcgaa aactaatccc gccagcgtcc
      661 ttggaggcac ctgcaccagc agggccgcat ggagctttcc atccctcagt ccgcgcaaca
      721 tttcctgcgt ggataaatcg tgaagctgca cgcgcacacc agggttggac tttcggaaat
      781 atctcagcgc acgcggcagg agttcgaccg tcaatgacgg cgcgtaacca atctgaattt
      841 cgccgcgctt tcctccggct acctcttttg ccatttgcac agctccctcc gcatgccgca
      901 aaatcaggcg agcctcagtg aggaaaacgc gaccggcttc agtaagccgc actgcatttg
      961 catcgtgatc aaaaagtgca acgccaagct catcctccaa attccggatt tgccggctta
     1021 acggcggctg cgacacgtga agccgcaacg ccgcacgcgt tacgttctgc tcttcggcga
     1081 cagccacgaa gtatcgcaga tgacgaagtt ccatttgcgc cagtataccc aatcggtatg
     1141 gtcggccaag caacaagata tttccgccaa atttaccctg caggtacagt gcaagcgatg
     1201 accgttcaag ttggaacgga gtaacacaaa cacaaaaacg aaaagacaga atctatggaa
     1261 gccaagatta ccaaaaatta ccatgagcgg ctcacgccgg acaatgccac tctacttctc
     1321 atcgaccatc aagttgggct gttcaccgga gtcagggaca tccctgttgc ggagctaaag
     1381 cataatgtcg tcgcgttagc gaaggcggcc cgggtattgg gcatgccgat catcgtgacc
     1441 gctacctcgc cggagatgtg gggtccaacc atcccggagc tgactgaagc cttgcccggg
     1501 gcttcagtca ttgcgcgaac aacggtcaat gcgtttgatg atgcccggtt cgctaaagcc
     1561 gtggaagcga ctgggcgcaa gaagctcatt atcgctggcg tttcaaccga agtctgtctg
     1621 gctttgcctg cgatttatgc gactggaatt ggctacgata cttacgccgt gattgacgcc
     1681 tccggcactt tcagcgaaac aaaacgcatc accggtctga tgcgcatggt gcaagcgggg
     1741 gtggtcgtca ccgactattc caccgttgcc gtggaaatgc tcaaagacaa cgcgagtcca
     1801 aaggcaggcg atctatatgc ggccctcggt atgccttttg ccggtttggt agggcaattg
     1861 gcgggagcat tcgccaagag ccggtccgcc tgaaccggct ggatacatcc gtggttgttt
     1921 agttctagtg ccacccaagc agccgcccgc cctggtaggt gataaaggcg agcgtccatg
     1981 ccagcgcacc catgtagagc cattggaaca ccggccattt ccatgaattc gtttcgcggc
     2041 gcacgatggc cacggtgctc acgcattgca gcgcaaaaac ataaaacacc atcaacgtca
     2101 cggcaagcaa cggcgtgtaa agtgtcgttc cgtcgggacg cttttggtcc ggcaggactt
     2161 tttgcaaatc tgccactttg tcgtccgctt cgccgtgtcc caaattgtaa accgtggaca
     2221 tggtgctgac gaaaacctcc cgcgcagcaa atgaagaaat gacgccgatg ccgatcttcc
     2281 aatcaaaacc gagcggagca atcgccggtt cgatcagcct tcccatacgc ccggcgaaac
     2341 tgtaacggag attttcgcct tgctcctggt tgtcgagcgc ggcgagttgt gcggtattgg
     2401 ttgaagcaat ggcggcccgt ttggcttcaa aatcgcgctt gacggcggga ttgtgcggat
     2461 acgtcgcaag gaaccagagc aagatgttga tgcccaaaat caccgtgccg gcgcgccgta
     2521 aaaaaagccg cgaacggtcc cacatgtgcc gcaaaacgac gcgcgcaaga ggacgtttat
     2581 agggcggcaa ttccatgatg agcatcggcg tttcgccttt gagcaaagtt tttttgaaaa
     2641 gccacgccat caggagtgca acaacgatac cgagcagata catcgtgagc atcgtcaatc
     2701 cggcgagttt gaaaaaaccg aatatgcgtt tgtccggtat gcacgcgccg ataagcagcg
     2761 tgtaaaccgg cagccgtgcc gagcaactca tcaacggcgc gacgagaatc gtcacgagcc
     2821 ggtccttggg gctttcgatg gtgcgcgtcg ccatgatgcc gggaatcgcg caggcaaacg
     2881 aactgagcat gggaatgaaa ctttttccgt gcaacccgac tttgctcatc aagcggtcca
     2941 ttaaaaatgc cgcgcgcgcc atgtacccgg tgtcttcgag caaaccgatg aacaggaaca
     3001 acagcaaaat ctgcggcaaa aaaaccacca ccgcgccgac gcctgcgatc accccatcca
     3061 ccagcaagct gttgagttcg cccggcggga tgagatgtcc cacaaagcct ccaatccggc
     3121 caacgacgaa cttcaacgcg tccatgggaa tgttggcgaa ggtgaaaatg ctttggaaca
     3181 tcagcgccat gatggcgacg aaaatcagcg ttccccaaac tttatgggtg aggatgcggt
     3241 caagcttgtc gctgaatgtt tcttcatccc tgcgcgtttc tgtcaccgcg ccttgataga
     3301 ctgccatgac acacgcgtaa cgcgcctcga tggccgcgct ccgccattcg attcccgacg
     3361 cttcgagtcg ttgccgcgcg gcttccaccg cttgctgaaa gaccgccgga taatgttcga
     3421 ggcttgaagc gagggccatc tcgttggtca aaatgagcag cgcttcggcc aaggcttgcc
     3481 ggtgatgttc gggaaaatgt ttttccaata attcggccag ggaattgatc tcgcgggaaa
     3541 agtcggacgg cagttcaaaa aattgtttgg gtgttttgag cgcaggtttc tcagaaagag
     3601 cggcagggat ctgttgacgc aattgcggaa ggccgcgtcc gctgctcgcc actatcggca
     3661 acgcgggcac gccaagagat gcggacaatt gggcggcatc aatttcgtgg ccattggctt
     3721 cggcgacatc caccatgttt aacgcaagca aggtgggatg gccgagttcg atgacctggg
     3781 tggcgtaata aagattgcgc tggagattgg aggcgtccac cacgaccacg atgagcgaag
     3841 gcgcgggaag ttccttgagc cgatgcagca agacgtcgcg cgaaatctgt tcgtcgaggg
     3901 attgcgggct gagac
//
LOCUS       DASZMM010000079         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3873531, whole genome shotgun sequence.
ACCESSION   DASZMM010000079 DASZMM010000000
VERSION     DASZMM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3873531"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /gene="purB"
                     /locus_tag="VG754_01995"
     CDS             complement(<1..1041)
                     /gene="purB"
                     /locus_tag="VG754_01995"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWF18005.1"
                     /translation="MIQRYSREQMRQIWSEQRKLEIWLQIELLASEALSHEGLVPPVD
                     FAQMKAKASFNLERCKELERTLNHDVIAFTTNVAENINSPASRWLHFGLTSSDVVDTA
                     FAVQMTQSADLLIDDVRELRQVIARKARKYQLTPMIGRSHGIHAEPTTFGLKLAVMYD
                     EFGRALQRLEAAKKAVAVGKLSGAVGTSAHLSPKVEAFVCKQLGLRPAPIATQVIQRD
                     LHAEFIGTLALVGASIERWATEFRHLQRTEVLEAEEFFAAGQKGSSAMPHKRNPITGE
                     RLTGLARVLRGNAVAALENVALWHERDISHSSVERIIFPDSCTLLDYMLVTLAKLTDG
                     LIVYPENMKRNME"
     gene            1662..2606
                     /locus_tag="VG754_02000"
     CDS             1662..2606
                     /locus_tag="VG754_02000"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWF18006.1"
                     /translation="MKVFSGTSNQPLAGKICEYMGIELGKCVVSSFPDGETFVKIEEN
                     VRGEDVYIIQSTSPPTNHHLMEMFIMMDAVRRASASRITAVLPFYGYARQDRKDQPRV
                     PITAKLVANLLVAAGASRVLTMDLHAQQIQGFFDIPVDHLYAAPVMYDYLKGLDLKDL
                     VVVSPDVGGLKMAHAYSQVLDTGLAIVAKRRKSAIEVESMTMIGEVRGKNVLMVDDLT
                     ETAGTLTAAAVLLKKKGAKRILACVSHAILNEIGIERLRKSNIDELITTDTVLRPAID
                     GLNITTLSVAGLLGEAINRINSNSSVTSLFEFKGGRTS"
     gene            2636..3406
                     /locus_tag="VG754_02005"
     CDS             2636..3406
                     /locus_tag="VG754_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWF18007.1"
                     /translation="MKSVSLNAYPRTQSRRGGVKKLRDGGRIPAIIYGRQAEPQSLEV
                     LAKELENLVHHSVSENLLVDLAVKDDKRPKRLALVQEVQHHALSGKMLHVDFHEVAED
                     EKVSIMVPVETTGEATGVKNGGGILEHVLFRVKVCALPKDLPEVIVVDVSHLEIGQAI
                     HIGDIKAVSGVEILGDKHISVIAVAAPISEAAEAAAAEAGATGTAEVEMIKEKKEEGA
                     EGAAPAAKAGEKAAAAPAAKAGEKAPAAGAEKKAEKKK"
     gene            3455..>3569
                     /locus_tag="VG754_02010"
     CDS             3455..>3569
                     /locus_tag="VG754_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWF18008.1"
                     /translation="MENLHLIVGLGNPGAKYARTRHNIGFMLVERLAEKWRT"
BASE COUNT          810 a          897 c         1061 g          801 t
ORIGIN      
        1 ctccatgttg cgcttcatgt tctcgggata aacgatgagg ccgtcggtca gcttcgcgag
       61 cgtgacgagc atgtaatcga gcagggtgca ggaatcggga aaaatgatgc gctcgacgga
      121 gctgtggctg atgtcgcgtt cgtgccagag ggcgacgttt tcgagcgctg ccacagcatt
      181 gccgcgcagg acgcgcgcga gtcccgtgag gcgttcgccg gtgatgggat tgcgcttatg
      241 cggcatggcg ctggaacctt tctggcccgc ggcaaaaaat tcttccgctt ccaaaacttc
      301 cgtgcgttgc agatgacgaa attctgtggc ccaacgctcg atgcttgcgc cgaccaatgc
      361 cagcgtgccg atgaattccg cgtgcaagtc gcgttggatg acctgcgtcg cgatgggcgc
      421 ggggcgcagg ccgagttgct tgcagacgaa ggcctcgacc ttgggcgaaa gatgcgcgct
      481 ggtgccgacc gctccggaaa gcttgccgac ggcgacagct ttcttggcgg cttccaaccg
      541 ttgcaaagca cggccgaatt cgtcgtacat caccgcgagt ttgaggccga aggtggtcgg
      601 ctcggcatgg atgccatggc tgcggccaat catcggggtg agttgatatt tgcgggcttt
      661 cctggcgatg acctggcgga gttcgcggac gtcgtcaatg agcaggtcgg cggattgcgt
      721 catctggacg gcgaaggcgg tgtccacgac gtcgctgctg gtgaggccga aatgcagcca
      781 gcggctggca gggctgttga tgttttcagc gacgttggtg gtgaacgcga tgacatcgtg
      841 gttgagggtg cgttcgagtt ccttgcaacg ctccaggttg aaagacgcct tcgctttcat
      901 ctgcgcgaaa tccaccggcg ggacgagccc ctcgtgggag agcgcctcgc tcgcgagcag
      961 ttcgatctgc agccagattt ccagcttgcg ttgctcgctc catatttgcc gcatttgctc
     1021 ccgcgaatag cgttgaatca tgagtcaatt aaacaattaa aaattagaaa tgaaaaatta
     1081 aaaatgtcgg aggtattttt ttacatttag agaaaaatca aatcagcccg ttcaatcgca
     1141 ttcttggaaa catcttcgac cgcgatccag aaatccttgt ctttgaacgt gggatgacaa
     1201 gccggaagat aaatttccta ttgtcggaag aatttatcaa ttgccgcatc gcatcgtcat
     1261 cctcgtcttc tccccattcc ttgcgcttcg attttttttg tgttcaaaaa cttcgcagcc
     1321 gcgcccatca agaatggctt gacttgcctt gactcgaatt cggataaaaa aggagtaatg
     1381 ccttgatgga tagacatttg tttcgatttt tgcatggctt gaccaaggct tgactgcctt
     1441 gaccgccaag cgacacagtt ggcccaatac ggaaaacatc gaacatcgaa cgctgaattg
     1501 gggaaacgtc ggtcggctct ggaacattgg attttcagga ttaagcgttc acaattgctc
     1561 ttgcaggatg gtttttggtt ggtaaagtca tcgaaccgtt ttgaaagtca ccgttgtcgg
     1621 tggctttttg ttttcgtgga cagcatacat tttacgcgat tgtgaaagtt tttagcggca
     1681 cgtcgaatca acctctggcc gggaagattt gcgaatacat gggcatcgaa ctggggaaat
     1741 gcgtcgtcag ctccttcccg gacggcgaaa cctttgtcaa aatcgaggag aatgtccgtg
     1801 gtgaggatgt ttacatcatt cagtccacct cgccgcccac aaaccatcat ttgatggaaa
     1861 tgttcatcat gatggacgca gtgcgtcgcg cgagcgcatc ccgaatcacg gcagtgctcc
     1921 ctttctacgg ctatgcgcgt caagaccgca aagaccagcc ccgtgtgccg atcacggcga
     1981 agcttgtggc caacctgctg gtcgcggcgg gtgccagccg cgttttgacg atggacttgc
     2041 acgcccagca aatccaaggg ttctttgaca ttccggttga tcacctttat gcagcgccgg
     2101 taatgtatga ttatcttaaa gggttggacc ttaaagactt ggttgtcgtc agcccggacg
     2161 ttggcggctt gaagatggcc catgcttact cgcaagtttt ggatacgggt ttggcaatcg
     2221 tcgccaaaag gcgaaaaagc gccatcgaag tggaatccat gacgatgata ggcgaggttc
     2281 gaggaaagaa tgtcttgatg gtggacgatt tgaccgaaac ggccgggacc ctgactgcgg
     2341 cggctgtgtt gctgaaaaag aagggcgcaa agcggatttt ggcctgtgtt tcccacgcga
     2401 ttttaaatga aattggcatc gaaagattgc gaaaatcaaa tattgacgaa ttaataacaa
     2461 ctgatactgt cctacgcccc gcgatagacg ggctgaatat cacgactttg tcggtggcgg
     2521 ggttgttggg cgaagccatc aaccggatca atagcaattc gtcggtgact tcgctgtttg
     2581 aatttaaagg cgggcgcacc agttaggtgt cgccgcgaga cggagcaagt aaaaaatgaa
     2641 atctgtatca ctgaatgctt atcctcgcac gcagtcccgg cgtggaggtg tcaagaaact
     2701 gcgcgatggc gggcgtattc cggccatcat ttacggacga caagccgagc cacaaagctt
     2761 ggaagtgctg gcgaaggaac tcgaaaacct ggtccaccat tccgtttccg aaaatttgct
     2821 ggtggacctt gccgtcaagg acgacaagcg tcccaagcgc cttgcgctgg tgcaggaagt
     2881 ccagcatcac gcactcagcg gcaagatgct gcacgtggat tttcatgaag ttgccgagga
     2941 cgaaaaagtt tccatcatgg tgcccgtcga aacgaccggc gaagccaccg gcgtgaagaa
     3001 cggcggcggc attctcgaac acgttttgtt ccgcgtcaaa gtgtgcgcgc tgcccaagga
     3061 tttgccggag gtcatcgttg tggatgtgag ccatcttgag atcggccagg cgattcatat
     3121 tggcgacatc aaagctgttt ccggcgtgga aatcttgggc gacaagcaca tctcggtcat
     3181 cgccgtggcc gcgccgatca gcgaagccgc ggaagccgcc gctgccgaag ccggtgccac
     3241 cggcacggcg gaagtggaga tgatcaagga gaagaaggaa gaaggcgcgg aaggcgccgc
     3301 tcctgccgcc aaggctggcg aaaaggccgc ggctgctcct gctgcgaaag cgggcgaaaa
     3361 agcgcctgcc gccggcgcgg agaagaaggc agaaaagaag aagtaatttt ctcacgcggc
     3421 ttggagcgat ccagccgccc ggcccgaccc ggccatggag aatttgcatc ttatcgtggg
     3481 gttgggaaat cccggcgcaa aatatgcccg gacgcggcac aacatcggtt ttatgctggt
     3541 ggagcggctc gcggaaaaat ggcggacga
//
LOCUS       DASZMM010000080         7134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5243662, whole genome shotgun sequence.
ACCESSION   DASZMM010000080 DASZMM010000000
VERSION     DASZMM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5243662"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1601
                     /locus_tag="VG754_02015"
     CDS             270..1601
                     /locus_tag="VG754_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015136619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HWF18009.1"
                     /translation="MSALSEFIREVAVPLTGAPYDYDPVLELVGNAQFVLLGEASHGT
                     HEFYRERAEITRRLIREKGFNAVAVEADWPDAYRVNRFVRGESKDAEAVDALDGFKRF
                     PQWMWRNADVLDFVGWLREHNDAQQSPERKTGFYGLDLYSLHASIEAVLAYLERHDPD
                     SAKRARKRYACFDHFGGDTQKYGMAAGLGVAQSCEEEVINELTELRLREAEYLQRDGR
                     VAADEFFSAEQNAFIIRNAEEYYRTMFRGEVSSWNLRDQHMMETLNSLVKHLKQFMRP
                     VKVVVWAHNSHLGNARATEMGERGEFNLGQLARERHGHHAVLIGFTTHHGTVTAASDW
                     DGPAERKHVRPALSESYEALFHEVGYPAFWLEFEKYPQTAMDLRGARLERAIGVIYRP
                     ETERISHYFMAQLPDQFDAVLHFDETRAVEPLERTAEWEAGHVEETFPSGL"
     gene            complement(1626..3698)
                     /locus_tag="VG754_02020"
     CDS             complement(1626..3698)
                     /locus_tag="VG754_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWF18010.1"
                     /translation="MKFHAALLFVGAVLSGTAFAQSSRPGWGSTPYHDASGTGVTFRV
                     WAPNATSVYVPGEFNGWSTTANPLGKEVTNGIWSGVWSGNISTASTGQQYKYFINYSG
                     GSVWKHDPRARLVTFSGTTSGANDIIYDPAAFNWKGDNLNPPALNDLFVYELHIGTFY
                     TNGAGSKFVSATNRLDYLKSLGVNAVEVLPIAEFPGDAGWGYNPAQPFAVENIGYGGA
                     DGFKTFVQACHLRGIAVLLDVVHNHYGPTDLDMWDFDGYTGSGPIGGGIYFWESNTNL
                     EITPYGDTRPNFSSNEVCNFIQDNFTMWLSEYHVDGFRWDTPGLMMNANNNGYITNAG
                     NLINAINSMIHTNYTGKISIAEDVYNSFGFDSAWDTTYPYSFTPVLTNTVDANRDMTA
                     VANAVQYNVRYGGAAGSGRVAFLEDHDVVGDLNNGVRLVTAIDPNTPNSYRARKLSTL
                     GAAVTIAAPGVPMIFQGQEMLENQSFDSSRSVDWTKTNTYSYIVRFYSDLIKARRDLK
                     GYTPGLEGNQCGIIQTDNVKKLIAFHRWKSAATKQDAVVIANFSGVTLSNYGLNFPYG
                     GNWHTHLNSDSTNYGSDYGNIGSFVVTASGPQNQANVTIGPYSLLIMSQTPDSPPQLS
                     VAASNGVVSVSWPSSYFEWILYSRASLTTNSAWAQVSPAQYQTNSSTVSVSITPSSGY
                     SFYRLQKM"
     gene            3874..4422
                     /locus_tag="VG754_02025"
     CDS             3874..4422
                     /locus_tag="VG754_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18011.1"
                     /translation="MLEHTFHRETIHRLARITLFGFLITFILSRISVFLIMADAMPNF
                     YFFMNGTHVHHLNYGIFLMSVVCGYSVFRRPMGRASEITALLYGVSLGLTFDEFGMWL
                     HLGGSYWQRASVDMVIVVAALLGLVGFATTLKSFEAKHFRASLILLIVLIGFGIALYM
                     AGQKIGTMVGPSLRDLEAASSP"
     gene            4431..5843
                     /locus_tag="VG754_02030"
     CDS             4431..5843
                     /locus_tag="VG754_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HWF18012.1"
                     /translation="MSQKTENLRAGVGRRCINPPPNIAHGGWGAQKHEQAEGIDMDLW
                     TTALACSDGNTTAVILDIDIQILTNERADQIRLAVSHATQLPLENIRASATHTHSGPV
                     PYKSWIEKGFEMVGPWFEHVAHRCTEATTEAISNLQPVTIRAGRGSCRINSNRRSVTA
                     TGERFLGVNPDGPCDHEVIIVKLDTIAQQPLATVVNFACHPTIMGPPNRLITPDYPGA
                     MKRVVEQAVGGHCLFLQGAAGDQGPLIGFQADVSVYRKFGAILGHEVAKVALELDHVP
                     ATVRFEKIIPSGAPLGMYAPEFATQASLPLSVLQKEISVPLREDLPKREVAAEKLDLW
                     KTRLKLAREKSDDAAITEAVYMARRADIQLRMAEDFGEATTANARTHFTTFGDVALVG
                     CNIEPFCEIGLNVKQQSPFPFTCMSGYTNGRLAYMATAEEWPKGGYEVENSPFGQKAA
                     EVLQKEIIATLHDLRRNPNH"
     gene            5848..6606
                     /locus_tag="VG754_02035"
     CDS             5848..6606
                     /locus_tag="VG754_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWF18013.1"
                     /translation="MNLADLTWPEVKKLKLTNKIVLFPLGSFEQHGPHLPLTTDTDIV
                     TAVARGLETKRPREILCLPTLWPGHSTHHLHFPGTLSVSQMPYIQLVIEMCRSIVNFG
                     AKKVFLLNGHGGNDVPVRAALRELKTEFPKVRFVFASYWSLAAQTIKQTRESAPGGLG
                     HACEMETSIMLHLCPERVKIRLAKRDGPRHKDIYRKSDMQYGRPVYFVNDFHEVTKTG
                     TIGQPELASAEKGKRFLDSIIAEVDTFVGEFLKW"
     gene            6608..>7134
                     /locus_tag="VG754_02040"
     CDS             6608..>7134
                     /locus_tag="VG754_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing protein"
                     /protein_id="HWF18014.1"
                     /translation="MKITRIETIPIRVPLKAQFAIRSGRGGAHLDSPFLLIKVHTNEG
                     IVGIGESSCTPRWSGEDQVTGAHLIRTYLEPLLVGESPLEVEVLTQKFRLAFAGNHFT
                     KAAVEMALWDIAGKAANKPLHELLGGKVREFVPTKWSVSGVGPDKAAEIARWAVAQGF
                     RSMKVKVGLEPNGDVA"
BASE COUNT         1629 a         1958 c         1946 g         1601 t
ORIGIN      
        1 cggcatctcg caaagagcaa ccgcaaattt ccggtctttt ttcggaaaaa gcgctccaga
       61 agtaatctgg tgccatttgc gggcaagcgt gtcgcggcat ggcaaaggcc accaaggggc
      121 atcaagtgca tagccctaaa aatctttttg ctgactgtgt cgcttcgtgt cgtttggcgc
      181 ggcgaggtcc aacttcgcgt tctgttcgat ggttgcggaa ggttgacatg ggcagcggcg
      241 ggcaggaagt tgacttggag gtttgtgtta tgagtgctct ttccgaattc atccgtgaag
      301 tggcggtgcc gctcaccggc gcgccttatg attatgaccc ggtgcttgaa cttgtcggca
      361 atgcgcagtt cgtcctgctg ggcgaagctt cgcatgggac gcatgagttc tatcgcgaac
      421 gcgccgaaat cacgcgacgc ctcatccggg agaaaggttt caatgccgtc gcagtcgaag
      481 cggactggcc ggatgcctat cgcgtgaacc gttttgtgcg cggcgaaagc aaggatgccg
      541 aggctgtgga cgcgctggac ggattcaagc gtttcccgca atggatgtgg cgcaatgccg
      601 acgtgcttga ttttgttggc tggttgcgcg agcacaacga cgcgcagcaa tcgcctgaac
      661 gcaaaactgg tttctatggc cttgacctct acagccttca cgcttcgatt gaagcggtgc
      721 tcgcctatct cgaacgccat gaccccgatt ccgcaaaacg cgcccgcaaa cgttatgcct
      781 gcttcgacca ttttggcgga gacacgcaaa aatatggcat ggccgccggc cttggcgtcg
      841 cgcagtcttg cgaggaggaa gtcatcaatg agcttaccga actccgcttg cgcgaggcgg
      901 aatatctcca acgcgacggt cgcgtggcgg cggatgaatt cttctccgcc gaacagaatg
      961 cgttcatcat ccgcaacgcc gaggaatatt accgcacgat gttccggggc gaagtttcat
     1021 cctggaacct tcgagaccaa cacatgatgg aaacgctcaa cagtctggtc aaacatctga
     1081 agcagttcat gcggcccgtg aaggtcgttg tgtgggcgca caactcgcat ctcggcaatg
     1141 cgcgcgccac agaaatgggt gagcgaggcg aattcaacct gggccagttg gcgcgcgaac
     1201 gtcatggtca tcatgccgtc ctcatcgggt ttacgactca tcacggcacc gtgacggcag
     1261 cgtcggattg ggacggaccc gccgaacgga aacacgtccg ccctgctctg tcggaaagtt
     1321 atgaagccct gtttcatgag gttggctatc cggccttctg gctggaattt gaaaaatatc
     1381 cgcagacggc aatggacctt cgcggcgcgc ggttggagag ggcaatcggg gtgatttacc
     1441 ggccggaaac cgagcgcatc agccattact tcatggccca attgccggac caatttgatg
     1501 ccgtgttgca cttcgacgaa acccgcgccg tcgaaccgct cgaacgaacc gcggaatggg
     1561 aagccggcca tgtcgaagaa acatttcctt ccggactcta gcgaaatcga aacctgcatt
     1621 ttcaactaca ttttttgaag ccgataaaaa ctatatccgc tcgatggggt gatgctcacc
     1681 gaaacggtcg aggagttggt ctggtattgc gccggagaaa cttgtgccca agccgagttg
     1741 gttgtcaacg aggcgcgaga gtacaatatc cactcaaaat aagaactggg ccaggagaca
     1801 ctgaccacgc cgttgcttgc tgccacgctc aactgcggcg gcgaatcggg cgtttgcgac
     1861 atgatcaaca ggctgtaagg gccgatggtc acgttggctt gattttgagg gccggatgcc
     1921 gtcacgacaa aactgccaat gttcccataa tcggaaccat agttcgtcga gtcgctgttg
     1981 aggtgcgtgt gccaattgcc gccataagga aaattcagcc cataattgct gagagtgact
     2041 ccggaaaaat ttgcgatcac aaccgcgtcc tgttttgttg cggcggattt ccagcgatga
     2101 aaggctatca gttttttgac attgtcagtt tgaatgatgc cgcattggtt tccctccaac
     2161 cccggcgtgt aacctttcaa atcacggcgc gctttgatga ggtcgctgta aaaacggacg
     2221 atgtagctgt aagtgttggt cttcgtccaa tccacggagc ggctcgaatc gaatgattga
     2281 ttttccaaca tttcctggcc ctgaaaaatc atcggcacac cgggcgctgc gattgtcacg
     2341 gccgcgccca aggtcgaaag ttttctcgct cgatagctgt tcggagtgtt cgggtcgatc
     2401 gccgtcacca gccggacgcc gttgttcaaa tcacccacca catcatgatc ttcaagaaac
     2461 gccactcgac cggaacccgc cgcgccgccg taacggacat tatattgcac tgcattggca
     2521 acggcggtca tgtcgcggtt ggcatccacg gtattggtca ggaccggcgt gaacgaatag
     2581 ggataagttg tgtcccaagc gctgtcaaaa ccaaacgaat tgtaaacgtc ctcagcgatg
     2641 ctgattttgc cggtgtaatt cgtatggatc atggaattga ttgcattgat gaggttgccc
     2701 gcgttggtga tgtagccgtt gttgttggca ttcatcatca gtcccggcgt gtcccaacga
     2761 aacccatcca catggtattc gctcagccac atggtgaagt tgtcctggat gaagttgcac
     2821 acctcgttgc tgctgaagtt cggccgcgtg tcgccgtaag gagtgatctc aaggttcgta
     2881 ttgctctccc aaaaataaat gccgccgcca atcggcccgc tgcccgtgta accatcgaaa
     2941 tcccacatgt cgagatcggt gggcccataa tggttgtgga cgacatcgag caagacggca
     3001 atcccgcgaa gatgacaggc ttgcacaaaa gttttgaatc catccgcgcc gccatagccg
     3061 atgttttcga ccgcaaacgg ctgcgcggga ttatatcccc agcccgcatc cccgggaaac
     3121 tctgcgatag gcaagacttc gaccgcattc acgccaaggc ttttcaaata atcgagccgg
     3181 ttcgtcgccg acacaaattt gctgcctgcg ccattggtgt aaaacgtccc gatgtgaagc
     3241 tcgtaaacga acaagtcgtt caacgcgggt ggattgaggt tgtcgccttt ccagttaaaa
     3301 gcggccgggt cataaataat atcgtttgcg ccggacgtcg ttccggaaaa cgtaaccaaa
     3361 cgcgcgcgcg ggtcgtgttt ccagacgctt ccgccgctgt aattgataaa gtatttatat
     3421 tgctgtcccg tcgaagcggt ggagatgttc ccagaccaga caccggacca gatgccgttg
     3481 gtgacttcct ttcccaaagg gttcgcggtg gtggaccagc cgttgaactc gccggggaca
     3541 taaacactgg tcgcattggg cgcccaaacg cgaaaagtaa ctccggttcc cgatgcatca
     3601 tggtatggag tcgaacccca acccggcctg ctcgattggg caaatgcagt gccgctcaac
     3661 accgcgccaa caaacaacag tgcggcatgg aacttcaaga ctttcagata caccatggaa
     3721 tataaaagag tgactcaatc caactggaat gtccaccgca tggacatgcg gaatggaagc
     3781 ttccaaattc acgctgctaa tttggaaaac attctgaaag ccggcctgtc atgcctgttg
     3841 aactctttgc gagccgatta taacctcatc gccatgcttg agcatacctt tcaccgggaa
     3901 acgattcaca ggctcgcccg aatcaccctg ttcggttttt tgattacgtt cattctttcg
     3961 cgcatcagtg tctttttgat catggcggat gcgatgccta atttttattt cttcatgaac
     4021 ggcacacatg tgcatcactt gaactacgga atttttctga tgtcggtggt gtgtggttac
     4081 agtgtctttc ggcgacccat gggaagggcc tcggaaatca ccgcgctttt gtatggagtt
     4141 tcattgggcc tgacattcga cgagtttggc atgtggttgc atctcggagg cagttactgg
     4201 cagagggcca gcgtggacat ggtgatcgtg gtggctgcgt tgcttggcct cgttggtttc
     4261 gcaaccacgt tgaagagttt tgaagcaaaa catttccggg cttccctgat cctcctgatt
     4321 gtgctgatcg gctttggaat tgctctctac atggctggcc aaaagattgg cacaatggtc
     4381 gggcccagct tgcgcgatct ggaagcggca tcatctcctt gaatcccacc atgagccaaa
     4441 aaactgaaaa cctgcgcgcc ggcgtcggca gacgttgcat caatccgccg ccgaacatcg
     4501 cccatggtgg ttggggcgcg cagaagcacg agcaggccga aggcattgac atggaccttt
     4561 ggaccaccgc gctcgcctgt tcggatggca acacgactgc tgtcattctg gacattgaca
     4621 tccaaatcct cacgaacgaa cgcgcggatc aaattcgttt agccgtcagc catgccacac
     4681 aattgccgct ggaaaacatt cgcgcatccg ccacacacac gcattccggc cccgtgccat
     4741 acaaaagttg gattgaaaaa ggcttcgaaa tggtcgggcc atggtttgaa catgttgcgc
     4801 atcgttgcac cgaggcaacc accgaagcca tctccaattt gcaaccggtc actatccgtg
     4861 caggacgcgg ttcatgccgt atcaattcca atcgccggag cgtcaccgca accggggaac
     4921 gtttcctggg cgttaatccc gacggccctt gtgaccatga ggttatcatc gtcaaacttg
     4981 acaccatcgc gcaacaacct ctcgccacag tcgtcaactt cgcttgtcat cccaccatca
     5041 tgggcccgcc gaacaggctt atcacacccg actatcccgg agcgatgaag cgcgtcgtcg
     5101 aacaggcggt tggtggacat tgtttgtttc tgcaaggcgc ggcaggtgat caagggcccc
     5161 tcatcggatt tcaagccgac gtttcagttt atcggaaatt cggcgcgatt ctcggacatg
     5221 aagtcgcaaa ggttgcgctg gaactcgatc atgttcccgc cactgtgcgg tttgaaaaaa
     5281 tcattccttc cggagcgccg ttgggaatgt atgctccgga atttgcgacg caggcatccc
     5341 ttccactcag cgttttacaa aaggagattt cggttccttt gcgcgaagat ttgccaaagc
     5401 gtgaggtcgc ggctgagaaa ctcgacctct ggaaaacccg cctgaaactc gcgcgagaaa
     5461 aatcggacga cgcggccatc accgaagctg tttacatggc gcgccgggct gatatccaat
     5521 tgcgcatggc ggaggatttc ggcgaagcaa cgaccgccaa cgcgcgcaca catttcacga
     5581 catttggcga cgtggccctg gtcggttgca acatcgagcc attttgcgaa atcggcttga
     5641 acgtcaagca gcagtcgcca tttcccttca cgtgcatgtc cggttacacg aacggacgcc
     5701 ttgcttacat ggccaccgct gaggaatggc cgaagggcgg ttacgaagtc gagaactcgc
     5761 cgtttggcca aaaggctgcg gaagttttgc aaaaggaaat cattgccacg ctccacgatt
     5821 tgcgccgcaa cccaaaccac tgaatccatg aacctcgccg acctcacctg gcctgaagtc
     5881 aagaagctca agctcacaaa caaaattgtt ttgttcccgc ttggttcctt tgagcagcac
     5941 ggcccgcatc tgccgctgac cacggacacc gacatcgtga ccgccgttgc acgcggactg
     6001 gaaacaaaac gcccgcgcga aatcctttgt ctgccgacgc tttggcccgg ccattcgacg
     6061 catcaccttc atttccccgg cacgttgagc gtgagtcaga tgccatacat tcagttggtg
     6121 attgaaatgt gccggtccat cgtcaacttc ggcgcgaaaa aagtttttct cctcaatggt
     6181 catggcggaa acgacgtgcc tgtgcgcgcg gcgttgcggg aattgaaaac cgaatttccc
     6241 aaggtgcggt tcgtgttcgc ctcctattgg tcgctggccg cgcagaccat caagcaaacg
     6301 cgtgaatccg ccccgggcgg actcgggcac gcctgcgaaa tggaaacttc catcatgctg
     6361 catctctgcc ctgagcgcgt taaaatacgc ttggcgaaac gcgatggccc gcgtcacaag
     6421 gacatctatc gcaagtccga catgcaatac gggcggcctg tttattttgt gaatgatttt
     6481 cacgaagtca ccaaaaccgg cacaatcggc caacccgaac tcgcatccgc cgaaaaagga
     6541 aaaaggtttc tcgacagcat cattgcggaa gtggacacat tcgtgggcga atttttgaaa
     6601 tggtgaaatg aaaatcaccc gcatcgaaac aattccgatc cgcgtcccgt tgaaagcgca
     6661 attcgcaatc cgcagcggac gaggcggagc gcacctggat tcgccttttt tgttgatcaa
     6721 agtccacacc aacgagggca ttgtaggcat tggcgaatcc tcctgcacgc cccgctggag
     6781 cggcgaagac caggtgaccg gcgcgcattt gattcgcacc tatctggagc cattgctcgt
     6841 cggggaaagt ccgctcgaag tcgaagtatt gacgcaaaaa tttcggctcg cgtttgcggg
     6901 aaatcatttc accaaagccg ctgtcgagat ggcgctttgg gacattgcag gcaaagctgc
     6961 gaacaaaccg cttcatgaat tgctcggcgg caaagtgcgt gagttcgttc cgacaaaatg
     7021 gtcggtctca ggtgttggac cggacaaggc agcggagatt gcccgatggg ctgttgcgca
     7081 aggttttcgc tcgatgaaag tcaaggtcgg ccttgaaccg aacggggacg tggc
//
LOCUS       DASZMM010000081        17873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4423039, whole genome shotgun sequence.
ACCESSION   DASZMM010000081 DASZMM010000000
VERSION     DASZMM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 17873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17873
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4423039"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..2488
                     /locus_tag="VG754_02045"
     CDS             176..2488
                     /locus_tag="VG754_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF18015.1"
                     /translation="MLNAGDALNLPAIAAGTSPVGYYWTDVNAGTNVAAGATNGLPLN
                     ATLTVGSIPGSWNGDQLELTVTNAAGSTNVYVSLTVFTNLTIANDLPASLSLLSGESY
                     SYSIGVVGPGPYFYQWYHAGAPVANQTNSTYTVAAGASGSTTYYVVVSNAFNSVTSSV
                     STLTTIAQLSNTYAAGVLLLNPAGYWPMHEVETAAPGAIETNYGTLGPLGTAYYSDWA
                     ADYGAFTRQVPGAIVGDADTALHFNYNVGNLANGAGTWTNEIYIPHTSPSSTLNPPFS
                     VECWLYNTNTSVGQLNQSVWGQHGWEGFNAGYAGGGTGGSTISGMQLAFSTAGITVYG
                     YFSGVQNIIVSAGASINVWTHVVVTCDANTNFTLYANGTAQATAAGVGKYTPDYWTPL
                     TIGGTRGGTRSALITVDEFAVYTNVISDISTHYNDGVGGGAGAYVHDVTNDNPVIYLR
                     MDAPPYTAPGTGNWPELINYGSVGNAGVYTPGTMPGVVAGPTTTNGGNYAGLSGTNVP
                     ALSGVSSFADAGYASAYNPTGSNANFTVTAMFRGNPCDNRVQSIVSHGINSWQLNVTT
                     NGTIVFNAGNGNTATSGTGNNPGDIETRGVYNDGKWHYVAAINSTNVVAIYVDGVLDT
                     SGTPSGVTPTSVIPGNPGHVMIGSDPSYTNNPVGVGRQFAGQVCEVAFFNQSMTATQV
                     QDLYKLAVTGTAVNPNPTNLTFSVAGSQLTLTWPMDHIGWTLQAQTNNLGVGISTNWL
                     DVPNSTLTNQVVIPIDPASGSAFYRLRYQP"
     gene            2594..3415
                     /locus_tag="VG754_02050"
     CDS             2594..3415
                     /locus_tag="VG754_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18016.1"
                     /translation="MESSRCIIEKRRLQDTAGFTLIELLVVIAIIAILAALLLPALSS
                     AKIKAKEIGCRNNLKQLGLAEQLYLNDSGGNMFQYPGGGTWLQLLRPVYANADQVSMC
                     PMTTVRSPLPGANTAGDYKTAWFYTYGSTNGSYTINGWLYAGNWSFAGVGPVSEAFKK
                     DSAINDTVRTPVFGDGIWPDAWPETNDLCNTHNLQTGTEADVTDGPAGMDRYLIARHG
                     PHRPSVPPTNANLAQPLPGGINMVFFDDHVEDVPLDNLWELYWHIDWSPRSRPSL"
     gene            complement(3509..6088)
                     /locus_tag="VG754_02055"
     CDS             complement(3509..6088)
                     /locus_tag="VG754_02055"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HWF18017.1"
                     /translation="MGLLVNGVSNPLAIDRDTTRFTWRLADTARGETQTAYQILVASS
                     LELLSGEKADRWDSGKVDSDQSASVEYDGTALSAATRFWWKVRIWDQTGKTSPYSAPT
                     YFDTGLAQGDWTSHFIWDGTTNQNNFAYFRKTFSVTHKPRLAKVYVTAHNDYLLYCNG
                     QLLGRGPARCDPYKYGQYNAYDITKLVKTGSNVFAAIGHWQGTWHDSGVNAKPAFLLE
                     ARLAYPDGSLSTIGTDGSWKALAHTAFIETNPTYFIAWFSNRAAIQFDSRLEPTGWHT
                     VGFDDSSWASATEVDRSDYHLFAQMAPLEHEQAELEPVNVTSKNGAWLVDFGRCIDGW
                     PKLTMRANRPGDIVRVEYYELTGERKSAGWDQYICHGGTETWKADFGRHTTFQVLKIT
                     GYAGKLETSDVRGIWAYCDADVAGHFNCSSPLLNSIYEMCERSARQNVQQAIISVDAN
                     REQSPWLADSWNIGNVLLYNDRNTMMIDKVVRDYAAEQMTNGSFHDCSPAGIFQCAEW
                     SMYWPMLLWEQYLFSGDKTLLREMSPRLTHFLAWIKAYQDPKTKLLNPRTARISDYAG
                     GITPNEGYNAVTACEYYQDLRIASRIFSVLGQTNQSDEYLRHAEEVKAGINSNLFNGE
                     FYLIRPDRKEMFPLASAWALRFDIEPPAQKSKILATILAPGDPVLGGYGGDAFYSGLW
                     NAGAGDFVVHDLVRYHLMLDQNKANWEQFGFSGEVNHAWTSYPGYLFLKYIAGIQPTS
                     GGFVTFDVRPETGGLTFAEGTVPTVKGLITIRWEKSAGGKFSLSVKVPANTRAAIYIP
                     KLVKGNFTITESGKVLWPANAKVKDPGVLAVSEEDSSVRCLVGAGNYRFSERPCVSSA
                     AHP"
     gene            complement(6314..7162)
                     /locus_tag="VG754_02060"
     CDS             complement(6314..7162)
                     /locus_tag="VG754_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18018.1"
                     /translation="MELIRGVKARWRAFTLIELLVVIAIIAILAGLLLPALAKAKQKA
                     KGIQCVSNMKQLQLCWHMYCDDNNDAMPPNGTPGILGSGVTTNSWITGNAQSDTTPQY
                     IESGLLYKYNTSPAIYACPSNNRRIPIANANDALYWHASVGTLEPQTRTCAIDLDCGG
                     FSASSPPGGVFTRNGATVRTLAKESDIKTPDPSQKIVFVDENEYSVDDGCFGIYPPGS
                     GVNDWWNLPGSRHNQGCTFSFADGRAEIWKWHGNAVITFTAYDQPADNSDDFPRVQAG
                     TSPYTP"
     gene            complement(7194..10748)
                     /locus_tag="VG754_02065"
     CDS             complement(7194..10748)
                     /locus_tag="VG754_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF18019.1"
                     /translation="MKTNTIATTIDPWRGTLTWSTAAILLVFLAFVAGTARAQTNIIY
                     QDTFARTGLLNGTAPDTVNASNAVWWASTNTLVNSALYTDGSEVIATNYPNPSNGYYI
                     NGFLPFTPQVGHKYTYAISAYGIIGGNQWLALGFALNEITNNYYAAANCGAAWLLVRA
                     SGANYQPFLGPGTTSGSTVTLPGGATTNTFTIVLDTTTGTAISGWTVSWYANGILGRQ
                     AVYGANPAIHYVGCGVDAATGYFQQFSLTDYAAPSTPPTISEQPQNATVSVGQKATFW
                     VNATGIPSPTYQWRTNSISGITNNITGATSVTYTTPALTPAYNALQYSVTVANFAGST
                     NSAPATLTVTNTPATVFSATKTASGAGVVVAFSKPVDPTTGLNAANYSLNNGASVLSA
                     SYGGTASNSVILTTTMLNTNSSYSLAVQSVQDLFGNTMTNTTAPVLPAGLAIYLRGDS
                     GVILDNSGNVAEWLDQTTNGNNMVQYILGGASARPALGSINSEPVLNFNSSSSNFLTA
                     ATSPSLAITNNMSIYAFVNFADLSAPREILSKTTGAQAAPYDYYASTGGNEVFYRGEG
                     QNSGQFTSAAGASAGASHVLAVMMTGVTSGSGSGTVTHYLDGNANGSGTLTVGNPSLI
                     ADTGNPLWLGGRNDEVQWMNGQIGEILIFNSALSDADRISVDNYLGLKYFSSYTIITD
                     LPASTTSSNGFAVTYTFAANWGSAHLALQWQENGTNIPGATGMSYTTPILSPSDNGDT
                     FDVAVMLPNGSTIYSTTNTLTVLNMPPYVTSAGIPIWSTTNIIVLFDEAVTPATATVA
                     ANYSLNNGASVLSASIGDTPNKLVLTTSALTWSANPGFYTLTIQNVKDLYGNTVVTGS
                     TPVGVYPQATALWVRADTGVTTNTDGTVTQWNDMSGNGNNLQDTTFFGFTDPLLTNSA
                     GGDMVVHFAATNGTYGSALYANDAASLRITGDMSVIAVVNFGTIAGGTNGEIISKTGQ
                     IHPNVPAPYDYYVNGTAALTYRGNGNTGTGNLGLFAATTGPSLGTPHIIAFSETGNTV
                     NHFLDGKAAGTGILNNSFSETNCGDQGQPVFVGGRGDFVNNHLSGDISELIVVGSSVS
                     KSDLTSLGYYLIAKHNLPIIIPSSNPTNIFASATGNQITLTWPMDHTGWQLQSNSVGL
                     MATNAWFTVAGSTATNQMIITPNPAKTNVFYRLFYQAQ"
     gene            11069..11965
                     /locus_tag="VG754_02070"
     CDS             11069..11965
                     /locus_tag="VG754_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWF18020.1"
                     /translation="MRSETASFYHYFPISERDKNWGLYATTAGEARIAPHTMYPPIGH
                     PKGYAFDWQHGRTLGGFALVYISSGQGKFESKPDFSVSIGPGHAFLLFPNVWHRYAPD
                     PETGWHEHWIGFDGAMARGWLRYRFISKKNPVVRINAEDTVLATFSRVMQAIRGNRPA
                     LQQILAGATDNLMGLIYSAQQTQPAAETQNSNAIQLAITRLQNEFERDLNMKLLAQEL
                     GVSYSWFRHRFTAHTGLSPHQYLLELRLVHARNLLAETEFSIKEIAMQTGFEDEFYFS
                     RLFRQKLNLTPSQWRSRSRHHK"
     gene            12076..13269
                     /locus_tag="VG754_02075"
     CDS             12076..13269
                     /locus_tag="VG754_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWF18021.1"
                     /translation="MKITDIKLYTVKSGDTGARGAAQGESQYWGGGWQAQTLISNPMS
                     VHPKYDRTRSLWMGGGQDPFVIEIFTDDGAGGFCANYGGGAFACSIIDQHLKRFLIGQ
                     DPFNIELIWDQMNRSTLPYGLGGVTSMAMAGVDLALYDLVAKAQNLPVYKIIGGQTKK
                     TIPCYVTIHPDYVEAWRDRGFLGVKIAAPWGVESGRDGIKKMEKLIAKLRKELGDDLE
                     IMIDCYLSWDIEFATRVAERVRDYDVKWFEDPLQNGWATEGNRILRDRISPILLATGN
                     LEFHYKAFHNILQHHATDIIQPEMQWCGGYTPTRWIAAMAKPHDMPVIPHGSSVYNYH
                     FVMANTNSPCAEYLSVGKGTEIQPIFSVLEGEPLPVNGKISLDPDKPGFGVELKRELL
                     APYKG"
     gene            13273..14391
                     /locus_tag="VG754_02080"
     CDS             13273..14391
                     /locus_tag="VG754_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF18022.1"
                     /translation="MKKVRIGIVGAGWWATSAHIPAVKSHPDAELVAVQSREKAKAER
                     IARDFGAKHACASLEQMLALKDLDAIIISSTPNVHYLQAKAALERGLHVLIEKPMTFT
                     ANEARELVEIAAQKKLQLLISCPWHFTAHSIEARRLIQSGALDEIKMISVLMTNPIDK
                     LLRGINTTPTHGLGNVYVEPRPGSYNDPAVAGGGQIYCQVSHAAAYLTFLTGLRAAEV
                     YARFDRDGSPNDIYNALTITLENGALVNLASTGATPLCERNYEVRIFGSKAILQLELW
                     HGRMTLIDFADRRTEFKPLSENEIYPGQSPALNLVDAILGKRPNGSPGELGLAAMEII
                     EAACESAQTNRCEKIREIKIRAGKKIGAGMRATVRENA"
     gene            14388..15566
                     /locus_tag="VG754_02085"
     CDS             14388..15566
                     /locus_tag="VG754_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWF18023.1"
                     /translation="MSRLVQLTGIAWDHSRAFPPLVATAQRYEETHPGIRIRWEKRTL
                     DEFGHTPIDQLAPKFDLIVIDHPWAGFAFEKNLVCDLKPLLPPAVWDDCAQNCIGMTF
                     ESYCHTGRLLALPIDAATPAPSWRPDLLEHAGVSVPATWAEAVALARRKLAVIPGFNA
                     DLFLHFLMLVKTFGAEPCAEPERIAPREIMRRGVEMLRELTEPMPRAIFDWNPIQVAE
                     RMTATDDFAWNAFAYTYNNYARDGFARARLCFGNLTLLESKGPRLRGVLGGTGIAISS
                     HSNNVEAALDYACFVAAGATQKTIYVHAGGQPSHRAAWNVPAVDALCGGFFSGTRATQ
                     EEAFVRPRYSGYVPLQTQGGSALQEALRDGRGAEAVLEKLDALYRESRRAGSTSFQAN
                     "
     gene            15572..16420
                     /locus_tag="VG754_02090"
     CDS             15572..16420
                     /locus_tag="VG754_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWF18024.1"
                     /translation="MKLKLCCADFSFPLLSHDSALKVIALLGLRGVDIGLFAGRSHLR
                     LEIELRNPAKRGATLRRRLARQGLAAADIFLQLHENFTDSALNHPDAARRKFARQQFL
                     KTLDYAAAAGSKHVTILPGVAFKTESRAASLLRATDELAWRVEQAVGMKLTVAVEPHV
                     GSITDTPKRALELASLVPGLGFTLDYAHFTRAGFPDARIEPLTARATHFHARCARKAR
                     LQSSFKDNAIDFRRALKALDHFHFNGWIALEYVWIDWEHCNETDVLSETIQLRDHLID
                     AAKKIS"
     gene            16426..17172
                     /locus_tag="VG754_02095"
     CDS             16426..17172
                     /locus_tag="VG754_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF18025.1"
                     /translation="MKKNWNGQKAIVTGGASGIGLAIARKLHVLGVKIVLCDLDSKKL
                     EAARKTIGQNCRTLCLDVTDLKAVETAVGNLVKQTGPVDILVNSAGITGITNVKSHQV
                     DPANVDLVFRVNFFGSFNTARAVLPGMLGQNYGRILHIASIAGKEGNAGMMAYSASKA
                     AVIGMTKVQGKELAETGITVNALAPAVIRTPMVGAMPRAQISYMTEKIPMKRCGTLEE
                     IADMAAFIVSPEASFTTGFAFDLSGGRATY"
     gene            17287..>17873
                     /locus_tag="VG754_02100"
     CDS             17287..>17873
                     /locus_tag="VG754_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HWF18026.1"
                     /translation="MNPHPFLGVFFHWLGGLASGSFYVPYRGVKKWSWETSWLVGGIF
                     AWIVCPWTLAFLMTNDLAGVLKQQTAGAIWWTYFFGLLWGFGGLTFGLTMRYLGMSLG
                     MGVALGYCAAFGTLMPPIFKALMPDLPVGTSIVEIAKSIPGQVTLAGVGICLLGIGIA
                     ALAGFTKEREMPDEQKKKAIKEFNFKKGIWVATFSG"
BASE COUNT         4032 a         4882 c         4945 g         4014 t
ORIGIN      
        1 agttatagga tccagaagct cgtcatccac gtccgacaat gacctgcagt ttaaggggta
       61 ccttgatgac gtgggggtct tcaactacgc cttaagcctg ggtcaaatcg tggcggagta
      121 ctccgcagtg gcaaatgtcc cgccatattt catccaaaag cctcctgcga gtataatgct
      181 gaacgccggc gacgccctga accttccggc cattgccgct ggaacatcgc ccgtgggcta
      241 ttactggact gatgtgaacg ccggaaccaa tgtggcggca ggggcaacca atggtcttcc
      301 gctcaacgca accctgacgg ttggcagtat tccgggcagt tggaatggtg atcaattgga
      361 attgaccgtg accaatgccg ccggttcgac gaatgtttat gtttcgctca ccgtctttac
      421 taatttgacg attgcaaacg atctgccagc atcactttca cttttgtccg gcgaatccta
      481 tagttattcg attggagtcg taggcccagg accttatttc tatcagtggt atcacgcagg
      541 agcgccagtt gccaaccaga caaattcgac ttacacggtg gcggcgggag cttcgggcag
      601 caccacttac tacgtggtcg tcagcaacgc cttcaactca gtcaccagtt cagtttccac
      661 tctcacaact attgcccaac tttccaatac ctacgcggcc ggcgttctcc tgttgaatcc
      721 tgcgggttat tggccgatgc acgaagtgga gaccgctgcc ccgggtgcca ttgaaaccaa
      781 ctacggcacg ttgggtcctt tgggcaccgc ctattactcg gattgggcgg ccgattacgg
      841 tgctttcacc cggcaagttc ccggggccat cgtcggagac gccgacacgg cactacactt
      901 caattacaac gtcggcaatc ttgccaacgg cgccggcaca tggacaaatg agatatatat
      961 tccgcatacg tcgccttcat ccactttgaa tccgccgttc tctgtcgagt gttggttgta
     1021 taacactaat accagcgttg gccaactaaa ccaaagtgta tggggccaac acggatggga
     1081 aggcttcaat gccggttacg ccggcggcgg caccggcggc agcaccattt caggcatgca
     1141 actggctttt agcactgccg gtattacggt ctatggctat ttcagcggtg tgcaaaacat
     1201 cattgtcagc gccggagcat caatcaatgt gtggacccat gtagtggtga cttgcgatgc
     1261 gaacacaaac tttacgcttt atgccaatgg cacggcacaa gcgactgcag caggcgtcgg
     1321 aaaatatacg ccggattatt ggacgccgct gaccattggc ggcaccaggg gcggaacacg
     1381 ctcggcgtta ataaccgtgg atgagtttgc ggtctatacc aatgtgatca gcgacatcag
     1441 cacacattac aacgatggcg tcggcggcgg cgcgggcgct tatgtccacg atgtaacgaa
     1501 cgataatccg gtgatttatc tgaggatgga tgcgccgcct tatactgcgc cggggacggg
     1561 gaactggccg gagttgatta actatggaag cgtgggcaat gctggagtct atactccggg
     1621 cacgatgccc ggcgtggtgg ctgggccaac caccaccaat ggcggcaatt acgccggatt
     1681 atcaggcaca aatgtgccag ccctgagcgg cgtgagcagt tttgctgatg cgggctacgc
     1741 ctcggcgtat aacccgaccg gctcgaacgc gaatttcacc gtcacggcca tgttccgcgg
     1801 taatccttgc gacaaccgcg tgcaatccat cgtcagccac ggaatcaatt cgtggcagtt
     1861 gaatgttacc accaacggca ccattgtctt taacgctgga aatggcaaca cggcaaccag
     1921 cggcactgga aataatccgg gcgatattga aacaagagga gtttataatg acgggaagtg
     1981 gcattatgtg gctgcaatca acagcacgaa cgtggttgct atttacgtgg acggcgtgct
     2041 ggacaccagt ggaacacctt cgggcgtaac cccaacgagt gtgatacctg gaaatccggg
     2101 ccatgtgatg attggctcgg atccgagtta cacgaacaat ccggtcggag tgggacgtca
     2161 atttgccggg caggtgtgtg aagtggcgtt ctttaatcag tcaatgaccg cgacacaagt
     2221 gcaggacctt tacaagctcg ccgttactgg cactgccgtc aaccccaatc caacaaacct
     2281 cacgttctcg gtggcgggca gtcagttgac cttgacttgg cccatggatc acattggttg
     2341 gacgctacag gctcaaacca acaatttggg cgtgggcatc agcaccaatt ggttggacgt
     2401 gccgaattcc actttaacca accaagttgt aattcctata gacccagcca gcggcagtgc
     2461 attctaccgc ttgcgttatc agccttagat ctgagcaaat caactttaag caaaatttga
     2521 tgccgtgaaa aaatcgttaa atgtccgcat aaagaaagca tcaaaattaa tagaagggcc
     2581 ggttggcggc acgatggaat cctcacgatg cataatcgaa aagcggcggc tacaggacac
     2641 ggcaggcttc acgctcattg aactgctggt agtcatcgcc atcattgcaa ttctggcggc
     2701 cctgcttttg ccagccttga gcagcgccaa aattaaagcg aaagaaattg ggtgcaggaa
     2761 caatctcaag caattggggt tggcggaaca actatatctc aacgacagcg gtggaaacat
     2821 gtttcagtat ccgggcggcg gaacgtggtt acagttgctt cgccctgtat atgccaatgc
     2881 ggatcaggtg tcgatgtgtc ccatgaccac agtccggtcg ccgctgccag gcgccaacac
     2941 ggcaggtgac tacaaaacgg cctggtttta tacctacggc agcactaatg gcagctatac
     3001 gatcaacgga tggctttatg ccggcaactg gtcgtttgcg ggtgttggac ctgtaagcga
     3061 agcattcaag aaggattccg caatcaacga tacggtccga accccggttt ttggagatgg
     3121 gatatggccg gatgcctggc ctgaaacgaa cgatctgtgc aacactcaca acttgcaaac
     3181 gggaactgaa gcagatgtaa ccgacggtcc cgcaggcatg gacagatacc tgatcgccag
     3241 acatggtccc catcgcccca gtgtcccccc aacaaatgcc aatcttgcac aaccgttgcc
     3301 cggcgggatt aacatggtct ttttcgacga tcatgtggaa gatgtgccgc tggataatct
     3361 ttgggaactt tactggcaca ttgattggtc accccggtca agaccttcgt tgtaaccccc
     3421 catctgcttg tgccacgcta tgcgcgcatc cattggtgtt gaatagcggt cgatagagtc
     3481 ctttgggaga aaatcggggc tgtgcctgtt aagggtgggc ggcagatgaa acacatggtc
     3541 tttcactaaa tctgtaattg ccggcgccca ccagacatct gacagaagag tcctcctccg
     3601 aaaccgcaag cactccgggg tctttgactt ttgcatttgc cggccacagg actttcccgg
     3661 attccgtgat ggtgaaattt ccctttacaa gtttgggaat ataaatggca gcccgtgtgt
     3721 tggcaggaac tttgactgaa agagaaaatt tgccgccggc gcttttctcc caccgaatgg
     3781 taatgagtcc tttcacggtt ggaaccgtgc cttcggcgaa agtcaggccc cccgtttccg
     3841 gccggacatc aaaggtgaca aatccgccgc tcgtgggctg aatgccggcg atgtatttca
     3901 ggaaaagata ccctgggtag gaagtccagg catggttcac ttcaccgctg aaaccgaatt
     3961 gttcccaatt ggccttgttc tgatcgagca tgagatgata gcggacgagg tcatgcacaa
     4021 caaaatcccc ggcaccggcg ttccacaaac cgctgtaaaa cgcatcaccg ccgtagccac
     4081 cgaggaccgg gtctcccggc gccagaatgg tggcgaggat tttggacttt tgcgcaggcg
     4141 gctcaatgtc aaagcgcaag gcccacgcgg aggcgagcgg aaacatttcc ttgcggtcgg
     4201 gccggatgag atagaactcg ccattgaaga gattggaatt gatgccggct ttgacttctt
     4261 ccgcgtgccg cagatattcg tcactttgat tcgtctgccc caatactgaa aatatgcggg
     4321 aggcgatacg caggtcttga taatattcgc aagctgtgac ggcattgtaa ccttcgttcg
     4381 gcgtgatgcc gccggcatag tccgaaatcc gcgcggtgcg gggattgagc agcttggttt
     4441 tcgggtcctg atatgccttg atccacgcca aaaaatgagt cagccgcggc gacatttcgc
     4501 gcaggagcgt tttgtcgcct gagaagagat attgttccca cagcaacatc ggccaataca
     4561 ttgaccactc cgcgcattga aaaattcccg ccgggctgca atcgtggaaa cttccgttgg
     4621 tcatttgttc ggctgcatag tcccggacca ccttgtcgat catcatggtg ttgcggtcgt
     4681 tgtaaagcag gacgttcccg atgttccaac tgtcggcgag ccacggggat tgctcgcggt
     4741 tggcatccac gctgatgatg gcctgttgaa cattttgccg ggctgaacgc tcacacattt
     4801 catagatgga gttgagcaag ggactcgaac aattgaaatg ccccgccacg tcggcatcgc
     4861 agtaagccca aatcccccgc acatcggaag tttccaattt gcctgcgtag ccggtgatct
     4921 ttagcacttg gaatgtggta tggcgtccga agtccgcctt ccaggtttct gttccgccgt
     4981 ggcaaatata ttgatcccaa ccggcggatt ttcgttcgcc agtcagttcg taatattcca
     5041 cgcgcacgat gtcgcccgga cgatttgcgc gcatcgtgag tttaggccag ccgtcaatgc
     5101 accgtccgaa atccacgagc catgcgccgt tctttgatgt gacgttcaca ggttcgagtt
     5161 cggcctgttc atgttccagc ggtgccattt gggcgaacaa atgataatca gaccgatcca
     5221 cctcggtggc ggatgcccaa ctggaatcgt cgaaaccaac cgtgtgccag ccggttggtt
     5281 ccaggcgcga atcgaattga atggctgcgc gattgctgaa ccaggcaatg aaataagtcg
     5341 ggttagtttc gatgaatgcg gtatgcgcga gtgctttcca agatccgtca gttccaatcg
     5401 tggacaacga accgtccgga tatgccagcc gggcttccag caaaaaggcg ggtttggcat
     5461 tgacaccgga atcgtgccag gtgccctgcc aatgaccaat ggcggcgaac acgttcgagc
     5521 cggttttcac cagtttggtg atgtcgtagg cgttgtactg gccgtacttg taaggatcgc
     5581 accgggccgg tcccctgccc agaagttgtc cattgcaata aagaaggtaa tcgttatgcg
     5641 cagtgacata aaccttggca agcctcggct tgtgtgtgac cgaaaatgtc ttccggaaat
     5701 aggcgaagtt gttctggttt gtcgttccat cccaaataaa atgcgacgtc cagtcgcctt
     5761 gagccaaccc cgtgtcaaaa tacgtcggcg cactatacgg actggtcttt ccggtttggt
     5821 cccagatgcg caccttccac caaaatcgcg tggccgcaga cagcgcggtg ccgtcatatt
     5881 caaccgaagc cgattggtct gaatcgactt ttccgctgtc ccatcggtct gccttttccc
     5941 ccgacaggag ttcgaggctt gaagcgacaa gtatttggta cgcggtttgg gtttctccgc
     6001 gagcagtatc cgccaatctc caagtgaatc gcgtcgtgtc ccgatcaatc gccagcgggt
     6061 tgctcacgcc attgacaagt aatcccacgg gagcagccgg tttggctaaa gttccgaaag
     6121 tgaataaact gataatgagt gcgaaaatcg agcgtagcat cccgggaaaa aacctcataa
     6181 aaagatagct aaatgagaac ccatcttata aatacaccac aataggcagg gcgaggctgc
     6241 tgccaagcca attccgtcca atttcttcat ttccagttcg cgagaacatt cgcccgctat
     6301 ttatgatatg gatttaggga gtatagggag acgtaccggc ttggacgcgc ggaaaatcat
     6361 ccgaattatc ggcaggctga tcgtaggccg taaaagtaat gacagcgttt ccatgccact
     6421 tccaaatctc ggcgcggcca tcggcgaagg aaaacgtgca gccttgatta tgacggctgc
     6481 cgggaaggtt ccaccagtca ttgactcctg aacctggcgg ataaatgccg aagcagccat
     6541 cgtccacact gtattcattc tcatccacaa aaacaatttt ttgtgatgga tccggagttt
     6601 taatgtcgct ttctttggcc agtgtccgaa cagtggcgcc gttacgcgta aagacgccac
     6661 cgggaggact gctggcggag aatccgccac aatcaagatc aatcgcgcat gtacgcgtct
     6721 gcggttcaag ggtgccgact gacgcatgcc aatacagagc atcattcgcg ttggctattg
     6781 ggatccgtcg gttgtttgag ggacatgcat aaatcgctgg cgatgtgtta tatttgtaaa
     6841 gcaagcccga ttcaatatac tgcggcgtcg tgtctgactg cgcatttcct gtaatccacg
     6901 agttggtcgt cactccgctg ccgagaattc ctggcgtccc atttggcggc atcgcatcgt
     6961 tattgtcatc acaatacatg tgccagcaca gttgcaactg tttcatgttg ctgacgcatt
     7021 gaataccctt ggccttctgc ttggccttgg ccagcgctgg caggagcagt cccgcgagaa
     7081 tggcgatgat ggcaatgacc acgagcaatt caatcagtgt gaaagcccgc cagcgcgcct
     7141 tcacgccccg tatcaattcc attgttttat gatttttcaa cttcatggct ggctcactgc
     7201 gcctggtaga acaggcgata gaatacgttg gtcttggccg ggttcggagt gatgatcatt
     7261 tgattggtcg ccgttgaacc ggcaacggtg aaccatgcgt tggtggccat cagcccgacg
     7321 gaattgcttt gaagctgcca gccggtatgg tccatcggcc aggtgagcgt gatttgattg
     7381 ccggttgccg aggcgaaaat attcgttgga ttggaactgg gaataatgat cggcaaatta
     7441 tgtttcgcta taaggtagta accgagcgaa gtcaggtcag acttggaaac ggacgagccg
     7501 acgacaatca gttcggatat gtcgccgctc aagtgattgt tgacgaagtc tccgcgtccg
     7561 ccgacaaaca cgggttgtcc ctggtcgccg cagttcgtct cgctgaagct gttgtttaga
     7621 atgcccgttc cggcagcttt gccatcaagg aagtgattaa ccgtgttgcc agtctccgag
     7681 aaggcgatga tgtgcggcgt gccgagggat ggaccggtcg ttgccgcaaa caggcccaaa
     7741 tttcccgtcc cggtattgcc attgccgcga taggtgaggg ctgccgtgcc gttcacgtaa
     7801 taatcatagg gcgcggggac attgggatga atttgtcctg tctttgatat gatttcaccg
     7861 ttcgtgcccc cggcaatcgt cccaaagttc accacggcga taacggacat gtcgccggtg
     7921 attctgagcg acgcggcatc attggcatac aaggccgagc catacgtccc gttggtggcc
     7981 gcaaaatgaa ccaccatatc tcctcccgcg ctgttggtaa gcaacggatc cgtaaagccg
     8041 aaaaatgttg tatcctgtag attgtttccg ttgccggaca tgtcattcca ctgggtcacc
     8101 gtaccgtctg tgttggtggt gacgccggtg tcggccctga cccaaagagc ggttgcctgc
     8161 ggataaacgc cgaccggcgt tgaaccagtg acaacagtgt tgccgtataa gtccttcacg
     8221 ttttgaatgg tcagtgtgta gaagccagga tttgcgctcc acgttaaggc agaggttgtg
     8281 agaacgagtt tgttcggcgt atctcctatc gaggccgaaa ggacgctcgc gccgttgttc
     8341 aacgaataat tcgcggcaac ggtcgctgta gcgggagtta ccgcctcgtc gaatagcaca
     8401 atgatattgg ttgtgctcca aatgggtatg cccgccgacg tgacgtaagg cggcatatta
     8461 agcaccgtca aggtatttgt ggtgctgtag attgtagaac cgttgggcag catcaccgcc
     8521 acgtcaaacg tgtcgccatt atcggacggg gaaagaatag gcgtggtgta agacatacct
     8581 gtggcgccag gaatgttcgt gccgttttcc tgccattgga gggccagatg cgcgctgccc
     8641 cagttcgcag caaacgtata ggtcaccgca aatccattgg acgaagtggt gctcgccggc
     8701 aggtcggtga taatggtgta tgaggagaag tattttagcc ccaaataatt atccacagag
     8761 atgcggtcgg catccgacaa cgctgaatta aaaatcaaga tttcccctat ctggccgttc
     8821 atccactgta cctcgtcatt ccgtccgcca agccagagcg gatttcccgt gtcagcgatg
     8881 agcgaaggat tacccacagt caaagtgcca gaaccattgg cattgccatc caaataatga
     8941 gttactgtgc cactgccact tccgctggtt acgcccgtca tcattactgc aaggacatgg
     9001 gaagcgccgg cggaggcgcc agcggcgctg gtgaattgac cgctgttctg cccttctcca
     9061 cgatagaaca cctcgtttcc gccagtgctt gcgtaataat cgtacggtgc cgcctgggcg
     9121 cctgttgttt tgctgagaat ttccctgggg gccgataagt ccgcaaagtt gacgaaggca
     9181 tagatgctca tgttgttggt gatcgccaag cttggcgaag tggccgctgt cagaaaattg
     9241 ctgctgctgc tgttgaagtt taaaaccggc tcactgttga tgctgccaag cgccggtctg
     9301 gcggaagcgc caccgagaat atactgcacc atattgttac cgttcgtggt ctgatcaagc
     9361 cattcagcca cattgccgct attgtccaag atcactcctg aatcacccct caaataaata
     9421 gccaggccag ccggcaaaac cggcgccgtg gtattcgtca tggtattgcc gaacaaatcc
     9481 tgaacacttt gaacggcaag agagtaactg ctgtttgtgt ttaacatcgt ggtggtgagg
     9541 atgacgctat tggacgcagt gcccccgtaa ctggcggaga gaacagaggc gccgttgttc
     9601 aaactgtagt tggcggcgtt taatcccgtc gtcggatcaa ccggcttgga aaaggcgaca
     9661 acgacgcccg ctccgcttgc tgttttcgtc gcggaaaaaa ccgtcgctgg cgtgttcgtc
     9721 accgtcagcg tcgctggagc gctgttcgtg gaaccggcaa aattggccac cgtcacacta
     9781 tactgtaaag cgttgtatgc cggtgtaagc gcaggagtag tgtaagtgac gctggttgct
     9841 ccggtgatgt tattagttat cccgctgata ctattggtcc tccactggta agtgggcgaa
     9901 ggtatcccgg tagcgttgac ccagaaagtg gccttctgcc ccacggatac cgttgcgttc
     9961 tgaggttgtt cgctaatcgt cggcggtgtg gagggcgcag cataatcggt cagggagaac
    10021 tgttgaaaat agccggtggc ggcatcaacg ccgcaaccca cgtaatgaat ggctggattt
    10081 gccccatata cagcctgcct ccctagaatc ccgttcgcat accaactgac ggtccaccca
    10141 ctaatcgcag ttccggtagt cgtgtccaga acgatggtaa acgtgttggt ggtggctccg
    10201 ccaggcaagg taactgtaga accactggtc gtcccggggc ccaggaatgg ctgataattg
    10261 gcgccgcttg cgcgtacgag cagccaggct gcgccgcagt tggcggcggc ataatagttg
    10321 ttcgttatct cgttcagggc gaagcccagt gccagccatt ggttccctcc aattatccca
    10381 tatgcgctga tggcgtaggt atatttgtgc cccacctggg gtgtgaacgg caggaagccg
    10441 ttgatgtaat aaccgttgga aggatttgga tagttggtgg caataacctc cgagccatcg
    10501 gtatagagtg cgctgttgac caacgtgttc gtggaggccc accaaacagc gttgctagca
    10561 ttcacggtat cgggagcagt accgttcaac aggcccgttc gcgcgaaggt gtcttgatag
    10621 atgatattgg tctgcgcgcg cgccgtgcct gccacaaatg caaggaacac aagaaggatt
    10681 gcagccgttg accaagtaag agttccgcgc catgggtcga tggtcgttgc aatcgtattt
    10741 gttttcatgg tgattgtatt tgggtgttag ttagggttgt ccaaaatgcg tcttctactt
    10801 tggggaaaat tttattgacc gtgatggttc atggatttgc cagcctggag acagacatca
    10861 tttgcgcctg cgggcaatcc agtgccgtag attgctcccg aattccgctt ctcttcggat
    10921 tcataattct ggatgtgggt gacggtgcaa catcccacaa tccgcccgga atcaacaatg
    10981 gagaaaatgg caattcacat ggacaaaatg gcttgcgaag ctggggactt gacttggcga
    11041 ccggagaaat caaaattcca gtcatcgcat gagatccgaa accgcatcat tttatcatta
    11101 ttttcccatc agtgagcggg acaaaaattg gggcctgtac gccaccaccg ccggtgaagc
    11161 ccggatcgcg ccgcatacga tgtatcctcc aatcggccac ccgaaaggct acgcgttcga
    11221 ctggcagcac gggcgtaccc tcggcggatt cgcgctggtt tatatttcca gcggccaagg
    11281 taaatttgaa tcgaagccgg atttctctgt ttccatcggg cccggccacg ctttcctgct
    11341 ctttcccaat gtctggcatc gctatgcgcc ggacccggaa accgggtggc acgaacactg
    11401 gatcggtttt gacggcgcaa tggcccgcgg ctggttacgg tatagattca tctccaaaaa
    11461 aaatcccgtc gtcagaatca acgccgagga caccgtgctg gccaccttca gccgtgtgat
    11521 gcaggcgata cgcgggaatc gtccggcgct ccagcagata ctggccggcg cgacggacaa
    11581 tctgatgggc ctgatttact ccgcgcaaca aacgcagcct gccgctgaaa cgcaaaattc
    11641 gaacgcgatt caactcgcca tcacccgtct tcagaatgaa ttcgaacgcg acttgaacat
    11701 gaaactttta gcgcaggaac tcggtgtaag ttatagttgg ttccgccaca gattcactgc
    11761 tcataccggt ttgagtccgc accaatatct gcttgaactt cggcttgtgc atgcccgcaa
    11821 cctgctcgcc gagaccgaat tctccatcaa ggaaatcgcc atgcagacgg gttttgaaga
    11881 tgaattttat ttctcgcgcc tctttcggca gaaattaaac ctgactccca gccagtggcg
    11941 cagccgcagc cgtcaccata aatagttttc aatcgttttc gcattgtcaa aaaatccatg
    12001 tctattgcca ttttatccat tgcgcgaaag ggcgcagtcg gggatttttt catgcgaaaa
    12061 ccacacatgt tttagatgaa aatcacggac atcaagcttt acactgtcaa atccggtgac
    12121 acgggcgcgc gcggagcagc gcagggcgaa tcccaatact ggggcggcgg ctggcaggcg
    12181 cagacgctca tctccaatcc catgtccgtc cacccaaaat acgaccgcac tcgttcgctc
    12241 tggatgggcg gcgggcagga cccgttcgtc atcgaaattt ttaccgatga cggcgcgggc
    12301 ggattttgcg cgaactacgg cggcggcgca tttgcgtgca gcatcattga ccagcatctc
    12361 aaaagatttc tcatcggcca ggacccattc aacatcgaac tcatttggga ccagatgaac
    12421 cgttcgaccc tgccctacgg tcttggcggc gtgaccagca tggcgatggc gggtgtggat
    12481 ctggccctct acgatctcgt cgccaaggcg cagaatcttc ccgtctataa aatcatcggc
    12541 ggccaaacga aaaaaacgat tccatgttac gtcaccattc atcccgacta cgtggaagcc
    12601 tggcgcgacc gcggatttct cggcgtgaaa attgccgcgc cgtggggcgt cgaaagcgga
    12661 cgcgacggca tcaagaagat ggaaaagctt attgccaaac tgcgcaagga actcggcgac
    12721 gacctggaga tcatgattga ttgttacctg tcatgggaca tcgagttcgc cactcgcgtc
    12781 gccgaacgcg tccgcgacta cgacgtgaaa tggtttgaag acccgctgca aaacggctgg
    12841 gccaccgagg gcaaccgcat cctgcgcgac cgcatttcgc cgatactgct ggcgaccggc
    12901 aatctcgaat ttcattacaa agcctttcac aacattctcc aacatcacgc caccgacatc
    12961 atccagccgg agatgcaatg gtgcggcggc tacacgccca cgcgctggat cgctgcgatg
    13021 gccaagcccc acgacatgcc ggtcatcccg cacggctcgt ccgtttataa ttaccatttc
    13081 gtgatggcca acacgaattc gccttgcgcc gaataccttt ccgtcggcaa gggcaccgag
    13141 atccagccga tcttcagcgt gctcgaaggc gagccgttgc cggtgaacgg gaaaatttcg
    13201 cttgatccgg acaagccggg attcggcgtt gaactgaaac gcgagttgct ggctccctac
    13261 aaaggttgaa ttatgaaaaa agttcgcatc ggcatcgttg gcgcgggttg gtgggcgaca
    13321 agcgctcata tccccgcggt caagtcacat ccagatgcgg aactcgtcgc cgtgcaaagc
    13381 cgcgagaagg caaaagcgga gaggatcgcg cgcgacttcg gcgccaaaca cgcctgcgcc
    13441 agcttggagc agatgctcgc actcaaggac cttgacgcca tcattatttc ctcgactccg
    13501 aatgttcatt acctccaagc caaggccgcg ctggaacgcg gcctgcacgt gctcatcgaa
    13561 aagccgatga cgttcacggc caatgaggcg cgcgaactgg ttgaaatcgc ggcgcaaaaa
    13621 aaattgcagt tgctcatcag ttgtccgtgg catttcaccg cccacagcat cgaggcgcga
    13681 cggctgattc aatccggcgc gctggacgaa atcaaaatga tttccgtgct gatgaccaat
    13741 cccattgaca agctgctgcg cggcatcaac accacgccga cgcacggact gggcaatgtc
    13801 tatgttgagc cgcgtcccgg ttcttataac gatccggcgg tcgctggcgg cgggcaaatc
    13861 tactgccagg tctcgcacgc ggcggcgtat ctgacgtttc tcaccggcct gcgcgcggcg
    13921 gaggtctatg cgcggtttga ccgcgacggc tcgccgaatg acatttacaa cgcgctcacc
    13981 atcacgctcg aaaacggcgc gctggtcaat ctcgccagca ccggcgcgac gccgctttgc
    14041 gagcgcaact atgaagtccg cattttcggc agcaaagcca ttttgcagct tgaactctgg
    14101 cacggcagga tgacgctgat tgacttcgcc gaccgacgca cggaattcaa accgctctcc
    14161 gaaaatgaaa tttatccggg ccagtcgccg gcgttgaatt tagtggacgc gattttgggc
    14221 aaaaggccga acggttcgcc cggcgaattg ggactcgccg ccatggaaat catcgaagcc
    14281 gcctgtgaat ccgcgcagac gaaccgctgc gagaaaatcc gcgaaatcaa aatccgcgcc
    14341 ggcaaaaaga ttggcgctgg catgcgcgcc acggtccgcg aaaacgcatg agccggcttg
    14401 tccagcttac cggcatcgcg tgggaccaca gccgcgcttt cccgccgctg gtagccaccg
    14461 cgcaacgtta cgaggaaacg catcccggca tccgcatccg ctgggaaaaa cgcacgctcg
    14521 acgaattcgg ccacacaccg attgatcagc tcgcgcctaa attcgacctg attgtcattg
    14581 accatccgtg ggctggattt gcctttgaga aaaatctggt ttgcgatctg aagccgctcc
    14641 tgccgccagc cgtgtgggac gactgcgcgc aaaactgcat cggcatgacc tttgagagct
    14701 attgccacac cggacgattg cttgcgttgc cgattgatgc cgccacgccc gcgccaagct
    14761 ggcgacccga tttgctggag cacgccggag tctccgtgcc ggccacatgg gcagaggcag
    14821 tcgcgctcgc ccgccgcaag ctggccgtga ttcccggttt caacgccgat ttgtttttgc
    14881 attttttgat gctggtgaag acattcggcg ccgaaccctg cgccgaaccc gaaagaattg
    14941 cgccgcgcga aatcatgcgg cgcggcgtgg aaatgttgcg cgagctgacc gaaccgatgc
    15001 ctcgcgccat ttttgactgg aatccgatcc aagttgccga acgcatgact gccaccgacg
    15061 attttgcgtg gaatgcgttt gcctacactt ataacaatta tgcccgcgac ggttttgccc
    15121 gcgcgcgact gtgtttcggc aatttgactt tgttggaatc aaaaggtccg cgactgcgcg
    15181 gcgtgctcgg cggaacaggc atcgcgattt caagccactc caataatgtg gaagccgcgc
    15241 ttgactacgc ttgtttcgtt gccgcgggcg caacacaaaa aacaatttac gttcacgccg
    15301 gcggacaacc gtcgcaccgc gcggcgtgga atgttccggc agtggacgcc ttgtgcggcg
    15361 gttttttcag cggaacgcgc gcgacccagg aagaggcatt tgtccgtcca cgttatagtg
    15421 gttatgtccc attgcagacc caaggaggca gcgcattgca ggaagcgctt cgcgacggac
    15481 gcggtgcgga agccgtgctg gaaaaactcg acgcgctcta ccgtgaatca cgacgagccg
    15541 gctcgacctc ctttcaagcc aattgacgcg gatgaaactt aaactttgtt gcgctgattt
    15601 cagttttccc ctgttgtctc acgactcggc gttgaaagtg attgcgctgc tcggactgcg
    15661 cggcgtggac atcggcttgt tcgcagggcg aagccatttg cgccttgaaa ttgaacttcg
    15721 caatcccgca aaacgcggcg caaccctgcg tcgccgcctc gccaggcaag gacttgccgc
    15781 agcggacatt tttcttcagc ttcatgaaaa tttcacagac tcggccctca atcatcccga
    15841 cgcggcacgg cgcaaatttg cccggcaaca gtttttgaag acgctggatt acgccgccgc
    15901 cgccggctca aagcatgtca cgattctgcc cggcgtggct ttcaagaccg aatcgcgcgc
    15961 cgcgtcgctg ctccgcgcga cggacgaatt ggcatggcgc gtcgaacagg ccgtcgggat
    16021 gaaactgaca gtggcggtcg agccgcacgt gggatcaatc accgacacgc cgaaacgcgc
    16081 gctggaactg gcttcgctcg tgcccggcct cggcttcacg ctcgattatg cgcatttcac
    16141 ccgcgccgga tttcccgatg cgcgcatcga accactcacc gcccgcgcca cgcattttca
    16201 cgcccgatgc gcccgcaagg cccgactgca aagcagcttc aaggacaacg cgattgattt
    16261 ccgccgcgca ttgaaggcgt tggatcattt tcatttcaac gggtggattg cgctcgaata
    16321 cgtctggatt gattgggagc attgcaacga aacggacgtg ctttccgaaa cgattcaatt
    16381 gcgcgaccat ttgattgacg ccgcaaaaaa aatttcctga acaccatgaa aaaaaattgg
    16441 aacgggcaaa aagccatcgt caccggcggc gcgtctggaa ttggactggc catcgccaga
    16501 aaacttcatg tcctcggcgt caaaattgtg ctttgcgatt tggattcaaa aaaactggaa
    16561 gcagcgcgca agaccatcgg ccaaaattgc cggacgcttt gccttgacgt gacggacttg
    16621 aaagccgttg aaaccgccgt cggcaacctc gtcaagcaaa ccggcccggt ggacattctg
    16681 gtcaacagcg cgggcatcac cggcatcacc aacgtcaaga gccaccaggt ggatccggcg
    16741 aacgtggatt tggtttttcg cgtgaacttc tttggctcgt tcaacaccgc gcgcgcggtg
    16801 ctgccgggaa tgttgggaca aaattatggg cgcattctcc atatcgcgtc catcgccggc
    16861 aaggaaggca atgccggcat gatggcttat tccgcgtcaa aagcggcggt catcggcatg
    16921 accaaagttc aaggcaagga actggctgaa accggcatca ccgtcaatgc gctcgcgcct
    16981 gccgtcatcc gcacaccgat ggtcggggcg atgccgcggg cgcaaatttc ctatatgacc
    17041 gaaaaaatcc cgatgaagcg ttgcggcact ctggaagaaa ttgccgatat ggccgcgttt
    17101 attgtttcac cggaagcgag tttcacgacg ggctttgctt ttgacctgtc cggcggccgg
    17161 gcaacttatt gaataaaaaa aacgcatgat gagaattatc cgctttcaac atactggcgt
    17221 gccacgcatg cgcgcggcca agaggcggag tgcgatgtcg agcacggatc ggtggtttga
    17281 gaagccatga atccccaccc gttcctcggc gttttcttcc attggctcgg cggactggcg
    17341 tcgggaagtt tctacgtgcc gtatcgcggc gtgaaaaaat ggtcgtggga aacctcctgg
    17401 ctcgtcggcg ggatttttgc ctggattgtc tgcccgtgga cgctggcctt tcttatgacc
    17461 aacgatttgg ccggcgtgct caaacagcaa accgcgggcg cgatttggtg gacctatttt
    17521 ttcggactgc tctggggttt cggcggattg acgtttggtt tgacgatgcg ttatctgggc
    17581 atgtcgctgg gcatgggcgt ggcgctgggc tactgcgcgg cgttcgggac gttgatgccg
    17641 cccattttca aggcgttgat gccggatttg ccggtgggca caagcatcgt tgaaattgca
    17701 aaatccattc ccggccaggt cacgctcgcc ggcgtgggca tttgcctgct cggcatcggc
    17761 attgccgcgc tggcgggttt cacgaaggaa cgcgaaatgc cggacgagca aaaaaagaag
    17821 gccatcaagg aattcaattt caaaaaaggc atttgggtcg cgacgttttc ggg
//
LOCUS       DASZMM010000082        13345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3332737, whole genome shotgun sequence.
ACCESSION   DASZMM010000082 DASZMM010000000
VERSION     DASZMM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13345
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3332737"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VG754_02105"
     CDS             complement(<1..864)
                     /locus_tag="VG754_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWF18027.1"
                     /translation="MQPSLLGIEIGGTKLQIVVGDSSAKILQTHRLAVDRASGGAGIR
                     RQIETTLAKILPDIQSFAAVGVGFGGPVDWRTGKICCSHQIEGWSEFDLAGWLKKLTG
                     APVVVDNDANVATLGEAIHGAGKNANPVFYVTLGSGVGGGLVVDGKIYHGATPGEAEI
                     GHVRLDRAGTIVESRCSGWAVDAKIRALKTTEPSSALCRMIGAETGGEARHLAAALSQ
                     NDAAAQRILRETAEDLAFGLSHVVHLFHPEIIVIGGGLSNVGEPLRAAVEKSLGSFVM
                     EAFAPGPRVCLA"
     gene            complement(919..2016)
                     /locus_tag="VG754_02110"
     CDS             complement(919..2016)
                     /locus_tag="VG754_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF18028.1"
                     /translation="MQSRRILFISMAVIFAAAFAWFVLKPREPSYQGKTLTAWLEEET
                     TNGVEIYPEADPAAINAIRQIGTNAIPTLLKLAAVRDTPFRRTLLKAAGMLNEKCGID
                     LHVNSHYELQALSCRGFFCLGPQSKPAVPGLIALLHDDDEGVCEVAIYDLGYIGPEAL
                     PAVPDLIKEFERETADTNESDFAAAWAIGKIGPSAKAAVPVFTEGLTNNSLPYRMTCQ
                     AALINLHAASITPIVEQLKDTTNMTQWHYAKTIALSCGTNASPAVPLFISALNCTNQR
                     IQSAALSSLGFIRERADISIPAIVPFLASKNDYSRHSALRTLRMFGKAAKPAVPELIT
                     CLSDPNILVRKTATNALREIDPEAAAKAGIK"
     gene            complement(2068..3138)
                     /locus_tag="VG754_02115"
     CDS             complement(2068..3138)
                     /locus_tag="VG754_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF18029.1"
                     /translation="MRQSRRILFISMAVIFAAAFAWFVLKPREPSYQGKTLTGWLEGF
                     YSSMQDRPDAINALHHISSNAIPTLLNYVAAKDSRLEKSLLKWNEKHPRMTLPLYPET
                     EKQELADQGFQILGPDAKSAVPQLIALLKDDDENVRLTAAYCLGCIGDEAKSAVPDLI
                     KEFEREESVPPNYNPAAACALGKIGPSARAAIPVLTAGLTNRSPNLVVQAALINLHAA
                     SITPIVEQLKDTTKQAQWIRAMLIARWCGTNASPAIPLFISALNSTNGMVVDTSLVTF
                     GYIHEQPETCIPVIVPFLASKNVWHRFNALESLRMFGKAAKLAVPELIRCLDDPELLL
                     RKTATNALREIDPEAAAQHGIQ"
     gene            3520..4335
                     /gene="proC"
                     /locus_tag="VG754_02120"
     CDS             3520..4335
                     /gene="proC"
                     /locus_tag="VG754_02120"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWF18030.1"
                     /translation="MSEKLTIGFLGAGKMGTALARGFIHAGLVGPEQIIASDPVEAAR
                     AAFGRDSGAKIAASNLEVVKFASVLVLAVKPDQVAGVLGDIRGQFTEKHLLISIAAGV
                     PLAKLEGGLAPGARVIRVMPNTPALVGASATAYAMGKSTLPEDAQLAQKLFAAVGIAF
                     SVKESLLDAVTGLSGSGPAYIYLMIEGLSDGGVAAGLPRDVATKLAAQTMLGSAKMVL
                     ETGQHPGALKDMVTSPGGTTIEGLHELEKGKVRGSLMNAVRAATEKSKKLGQA"
     gene            4542..5048
                     /locus_tag="VG754_02125"
     CDS             4542..5048
                     /locus_tag="VG754_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HWF18031.1"
                     /translation="MTYQRFEDLPVWQKAIELAEQVYDLTEAPGFKGSFSLRDQIERA
                     AISVSNNIAEGFERGTTKELLAFLYVARGSAGEVRSMLCFMERRKAFQNFKSQISNLK
                     STAEACSRQLRGWAGSLQDSDIKGVRHLNQAALKAKEQKEKAVAMQKRLLANLPANHP
                     LRRNAAEL"
     gene            5088..6410
                     /locus_tag="VG754_02130"
     CDS             5088..6410
                     /locus_tag="VG754_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWF18032.1"
                     /translation="MSFPPPTPKQARVLWSCLTSLAVTLLLLLGGLLIWGLGWVLHKL
                     SAILVPMIFALILAYILDPVVEFFQRKRLPRIWSVSLVFALAALLLVAVLGSVLPGLR
                     RESKKLIDDLPKTSALIRTKIDSFLNDSALGKQLPDSWRDPLKSVVAQSPTNKSATSS
                     NTPEFLPSTNAIPDTTISTNSTDLATGAKTTDSGQISNKPVLPGLTGALVFVTKWFYA
                     QLSKISTWAEFLIGFILVPVYLFYFLLEKKSITSRWPDYLPLKPSRAKDETVFVLRAI
                     NDCMIVFFRGQVLVALCVGVLLAAGYSLMGLNYAVLLGLVAAVLGIVPYLGTITSLLL
                     ALTVAGVQFQDWSHPFAVLAIAALVKLLEDFIIGPCIIGERAGLHPLTIILAVLIGTT
                     LLGGFIGALLAIPLTAALRTLMFRYIWIHEHPRAKPSEVRKELEPLGK"
     gene            6482..6555
                     /locus_tag="VG754_02135"
     tRNA            6482..6555
                     /locus_tag="VG754_02135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6514..6516,aa:Gly,seq:ccc)
     gene            6759..9308
                     /locus_tag="VG754_02140"
     CDS             6759..9308
                     /locus_tag="VG754_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWF18033.1"
                     /translation="MKSKTLKQFVFIGMASLFLPMVTQAATIWNGPPVSESDATEPDQ
                     ITARVWLTRGSSEGLYNAVTETGFTHFFSPADTEWADGTTANYASLSYTDWNTWAKNI
                     HGGPPNTVGVNAVVHLITDDIYIDIMFTSWSTGGSYSYQRATPPAADVPPTIAITSPT
                     NGASFAAPGIVSITASASDSDGSVTNVAFFDGATFLGQTNNTPYTVTASLAAGSHALT
                     AVATDNAGLSTTSAIVNVTVSAANVPPTVTITNPPNNAVFGNTDTITVQTSASDSDGT
                     VTNVQLFNGTNLLRSFTNAPYNFSGTALAGNFALGTNILTAVAKDNLGATTTSAPVHV
                     IVARYTAELTNGAFSVFLIPVATNLSAPLYGVSPPSDTHRLFVLEQNGLMWIIQDGVL
                     LPAPALDLRSLVQPPLIANNANDERGLLGMAFHPGFNNPASLGYRTVYTYNSELIPSN
                     TLPTYPLSGGTSNLYKNALNEWKISITNANVIDPSTRREIISFGKNALNHNGGTCTFG
                     PDGYMYLALGDGGNANDVGPGHISPGGNAQNLSVPLGKMLRLNPLNPALNPSSSDPIS
                     SNGQYRIPASNPFQGPGQVPEIYAYGFRNPYRFDFDPVTGDLIEADVGQNNIEEINRV
                     VMAGNYGWAIKEGDFLFNMTNGTVGTPPGNFSPGIPTGLIDPISGPKGILEYDHNNGI
                     SITGGFVYRGAGMPELYGKYIFGDLALVANPVRENGRLFYADLQQGTINAFTLPQFGS
                     EILPNGDTVHGFGQDGSGELYAMATDTPPNGNGGVVYKLASLRLTLQENGNQLDISWP
                     TIAGHLQAQTNNSTGGLGTNWVNVPGSAATNHVTVSINPSNGSVFYRLVVP"
     gene            complement(9337..12564)
                     /locus_tag="VG754_02145"
     CDS             complement(9337..12564)
                     /locus_tag="VG754_02145"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HWF18034.1"
                     /translation="MAKASDGVVFFGLVLTGLIASCCPGSCADVVIQPAQTFQTITGW
                     GHGGGVLGGTAGAESMLPQAVADPVNYQYLDYLTDDLGLTGTRTWEVGPRIDGTGTDD
                     GDCDFVDWNLFEADTFSAQDAAYLVHYQNRILAEGFQPSFYSSPGYPTHASDSKPWVM
                     NHPGERAQQIWASSLYLKTNYGLNISYAVIYNEPSMAYTILTDDIKALGPRFLAQGLT
                     TRVQYAEAVAPQTDWNYITPALSDPDLWKYVGRISYHNYGTADPYRSYLRDFGNARGL
                     GTAQTEMGNPTFDDLFNDLTLAGVTYWEVAYSGGNTLANNAGLTAFTPSGTFFRLRQL
                     MHYVQPGAVRIGAASSDPSLRVLAFSQNGKVTTVIENTSPSAQTINLSALPPGSYGLS
                     QAVSGASSFQELGVRTIGTNGLLTLTNVAGNSAVTTLYPRAGTNQPPTIEVWSASPGY
                     LVAPTNSATLSIMASDPELATLTYHWSVTNQPAGANAVFANSNAPTTVVNGLTAAGNY
                     IFNADVSDGTNVSSRQLYLEVYGSNPPPVLGQTGFRIAAPYGLVFGDPGGMTHATIEL
                     PTSSAVLQAGISDLANSDFTGRGTWSLVSQPAGANAGVSATTYIFVSLRANVANMTVP
                     GDYLFQINVTNPGHSDLTAQILCTVHTATSPPVISSITASPPGVTLPANSLQLMAVTS
                     GSTNQPLRHWWAIKTVPPGAEPQLDHQGATNTAVGNLLIPGSYTFTLRAFDDLHMTTQ
                     DKTISVSPAPNAPVINSAASASIIVGMPFTYNITASFSPTGFSAMNLPPGLTFTNNVI
                     SGRPAMVGTYNIQLAATNASGAGYGNLALTVKLPLSVITSSAAADGLVNSPFNYTIQA
                     TSVATSFSATGLPPGLTLNSLTGAITGTNTTSGTFNVTITAANSTGTTTNSMTMVVYN
                     GAAPAPGITSVLTAMGNVAASFSYQITASNTPTSFFVIGLPAGLSFDPAGGRIFGVPA
                     VTGSFAVTLRAINRNGTGNAVLALAINPPPPPRIDAVAFQNGLSLSFLTLTNHYYSVE
                     WNTNLSNTNWDTLISGITGNAATQTVIDPVTNAQARFYRLKVIGP"
     gene            12955..>13345
                     /locus_tag="VG754_02150"
     CDS             12955..>13345
                     /locus_tag="VG754_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18035.1"
                     /translation="MKANQQIKQIIPAGIRTAFVGLFFVLWFCADVLAQDESSFNTPG
                     GVHYNADGTLSLQWTGPGTLESAPGLAGPWTAVSSGSSYAGNATVPASGTAGFFRLHS
                     GSRIGAVFPAPVIPALPAVQSATIQRLN"
BASE COUNT         3008 a         3692 c         3617 g         3028 t
ORIGIN      
        1 cgccagacaa acgcgcggtc cgggcgcaaa cgcttccatg acgaatgaac ccaacgattt
       61 ctccacggct gcccgcaacg gttcgcccac attcgacaat cccccgccaa tgacaatgat
      121 ttcgggatga aacaaatgca cgacatgcga aagcccgaac gcgaggtctt ccgccgtttc
      181 gcgcaaaatc cgttgcgcag cggcatcgtt ttgcgagagt gcggctgcga gatgacgcgc
      241 ttcgccgccg gtttctgcgc caatcattcg gcataacgcg ctggaaggct cggtggtttt
      301 gagcgcgcgg attttggcgt ccacggccca gccggagcag cgggattcaa caatggttcc
      361 agcgcggtcc agccgcacat ggccgatctc cgcctcgccg ggcgtcgcgc cgtgataaat
      421 ttttccatcg accacaagcc cgccgccaac gccgctgccc aacgtcacat aaaaaaccgg
      481 gttcgcattt ttgcctgcgc cgtgaatggc ttcgccgagc gtggcgacgt tcgcgtcgtt
      541 gtccacgacg acgggcgcgc cagtgagttt tttcaaccag cctgcaagat caaattccga
      601 ccagccttca atctggtgcg aacaacagat tttgcccgtg cgccaatcca ccggcccgcc
      661 aaacccaaca cccactgcgg cgaaggattg gatgtctggc aaaatttttg ccagcgtggt
      721 ttcaatctgt cggcgaatgc ccgcgccgcc cgacgcgcgg tccaccgcaa ggcgatgcgt
      781 ctggagaatt tttgcgctgc tgtcgcccac gacgatttgc agcttggtgc cgccgatttc
      841 gatgccgagc agggaaggtt gcatggaatt ttcagccaca gattaaacac ggatggaaca
      901 cggattcaaa ccgcaagatc actttattcc cgccttcgcc gccgcttcgg gatcaatttc
      961 acgcaaggcg ttcgtggcag tttttcggac aaggatatta gggtcgctga ggcaggtaat
     1021 taattccggc acggctggct tggcggcttt tccaaacatt cgcaaggttc ttaatgcgct
     1081 gtgcctggaa tagtcgtttt tggaagctag gaacgggaca atggctggaa tggatatgtc
     1141 agctcgttca cgaatgaaac ccaaactaga aagagccgcg ctttggattc tctgattggt
     1201 acagttcaat gcggagatga acaatggaac tgcaggactg gcatttgttc cgcacgaaag
     1261 cgctatcgtt ttagcataat gccactgagt catgttcgtt gtgtctttga gctgctcaac
     1321 aatgggagtg atcgaggcgg cgtgcaggtt gatcaaagcg gcttggcaag tcatccgata
     1381 tggaagcgaa ttgttggtca gtccttctgt aaaaacgggg acggcggctt ttgcggacgg
     1441 gccgattttg ccgatggccc aagcagccgc aaagtcactc tcgttcgtgt ccgccgtctc
     1501 gcgctcaaat tctttgatca aatcgggcac tgcgggcaga gcctccgggc caatataccc
     1561 caaatcgtaa atggcgactt cgcagacccc ttcatcatca tcatgaagca gcgcaatgag
     1621 gcccgggacg gcaggtttgg attgcggccc caagcaaaag aatccacggc aggacaaggc
     1681 ctgcagttcg taatgtgaat taacgtgtaa atcaatcccg cacttctcgt ttagcattcc
     1741 tgcggccttc aataaggttc ttctaaaggg cgtgtccctg acagcagcca gcttcaacag
     1801 cgtcggtatg gcattggttc caatctgccg gattgcattg attgcagcag gatcggcttc
     1861 gggataaatt tcgactccgt tcgtggtttc ttcctccagc catgccgtca acgttttccc
     1921 ctgatacgac ggctcgcgcg gtttgagcac gaaccatgcg aatgcggccg cgaagattac
     1981 cgccatgctg atgaaaagga ttcggcgcga ttgcatgaga tcagccacag attaaacaga
     2041 gacgaaacac gggttcaaag gacaagatca ttgtatcccg tgctgggccg ccgcttcagg
     2101 atcaatttca cgcaaggcgt tcgtggcggt ttttcgcagt aagagttccg gatcgtccag
     2161 gcagcgaatc aattccggca ccgccagctt ggcggctttt ccaaacattc gcaagctctc
     2221 taatgcattg aatctgtgcc agacgttttt agaagcgagg aacggaacga tgactggaat
     2281 gcatgtctcg ggctgttcgt ggatgtaacc gaaggtaaca aggctcgtat cgactaccat
     2341 tccatttgtg gagttcaatg cggaaatgaa caatggaata gcaggacttg catttgttcc
     2401 gcaccagcgt gcgattagca tcgcacgaat ccactgagct tgtttggttg tgtctttaag
     2461 ttgctcaacg atcggggtaa tcgaagcggc atgcaggttg atcaaagcag cttggacgac
     2521 gaggtttggc gaacggttcg tcaagcccgc cgtgagcact ggaatcgcag cgcgggcgga
     2581 tgggccgatt tttcccaaag cacaagccgc ggcagggtta taattggggg ggacggactc
     2641 ttcccgctcg aattccttta ttaaatccgg gacggcagat ttcgcttcgt cgccaatgca
     2701 tccaaggcaa taggcggccg taaggcgaac attctcatca tcatctttca gcaaagcaat
     2761 cagttgaggc acggcagatt ttgcgtccgg cccaaggatt tgaaaacctt gatcagccaa
     2821 ctcctgcttt tcggtttcgg gatacaacgg caaggtcatt cgcggatgtt tctcgttcca
     2881 tttcaacaat gatttttcta accgtgaatc ttttgccgcg acataattca gcagggtcgg
     2941 aatggcgttg ctgcttatgt ggtgaagcgc atttattgcg tccgggcggt cttgcattga
     3001 actgtaaaag ccttccagcc acccggtaag tgtcttcccc tgatacgacg gctcgcgcgg
     3061 tttgagcacg aaccatgcga atgcggccgc gaagattacc gccatgctga tgaaaaggat
     3121 tcggcgcgac tgcctcatgt ggaaattatg atttcagtcc gtgcacgaaa cgagaaaata
     3181 tttcgcaaaa acagcgcctt ggacagtctt tgtccgaggt ccgcatgtaa tatcgtgctt
     3241 ggaaaggtat gaacatgata agaacagatt cagagtggat tggacttgac cagtacccgc
     3301 tggaaggcga ggggctgcgg ccttcacaaa aggcccgtat tgattacttt tttcgcattt
     3361 tttcgcattt tttcgctttt tttggatgag ggggaggagg cttacaatag ttccaagttt
     3421 atggcaagtt acggttcaac caacctggag tgggcggtgc ttggaattac attgcctttt
     3481 ggctttcgct tgagtggctg acctgttaca aaacccgcca tgtctgaaaa actcaccatc
     3541 ggctttctgg gcgcgggcaa aatgggcaca gcgctggcgc ggggattcat ccacgccggg
     3601 ttggttggcc ctgagcaaat catcgccagc gatcccgtcg aggcagcgcg cgcggctttt
     3661 ggtcgcgatt ccggcgcgaa aatcgccgcg tccaatctgg aagtggtcaa gttcgcaagc
     3721 gtgctggtgc ttgccgtcaa gcccgaccag gtcgccggcg tgcttggaga cattcgcgga
     3781 caattcaccg agaaacatct tttgatctcc atcgccgcag gcgtgccgtt ggcgaaattg
     3841 gaaggcggtc ttgcacccgg cgcgcgcgtc atccgtgtga tgcctaacac gcccgcgctg
     3901 gtcggcgcgt ccgcgaccgc gtatgcgatg ggcaaatcca cgctgcccga ggacgcgcaa
     3961 ctcgcgcaaa aactttttgc agccgtgggc atcgcgtttt ccgtgaagga atcgttgctg
     4021 gacgccgtga ccggcttgag cggcagcggg ccggcgtata tttatttgat gatcgaggga
     4081 ttgagcgacg gcggcgtggc ggcgggtttg ccgcgagacg tcgccacgaa acttgccgcg
     4141 cagacgatgt tgggcagcgc gaaaatggtc ctggaaacgg gccagcatcc cggcgcgttg
     4201 aaagacatgg tgaccagccc gggcggaacc acgattgaag gattgcacga gttggaaaag
     4261 ggcaaggtgc gcggctcgct gatgaatgcc gtccgcgccg cgaccgagaa gtcgaagaaa
     4321 ttggggcaag cttgaaagca atttcagatt tgagatttca aatttgaaat cgatgccaga
     4381 tttgaaatcg aacgtctgat ttgaaatctc aaatctcgaa tttgaaatca aatggccgat
     4441 tcgaaatctc aaatttgaaa tctgaaatca aaaatctttt gagaagtgtt tgtaatctga
     4501 aatcacaata agcaaaaatc aaccaacgaa agggaaacat tatgacctat cagcgctttg
     4561 aggaccttcc ggtttggcag aaggccatcg aattggcgga gcaggtttat gatttgaccg
     4621 aagcgcccgg tttcaaaggc tctttcagcc tgcgtgacca gattgaacgc gcggcaattt
     4681 ccgtgtccaa caacatcgcc gagggatttg aacgcggcac cacgaaggaa ttgctggcgt
     4741 ttctttacgt tgcgcgcggt tcggcgggag aagtccgctc gatgctttgt ttcatggagc
     4801 ggcgcaaggc gttccagaat ttcaaatcac agatttcaaa tttaaaatcc accgcggaag
     4861 cttgctcgcg ccaattgcgc ggatgggcgg gttcgttgca agattcggac atcaaaggtg
     4921 tgcggcatct gaatcaagcc gccctcaagg caaaggaaca aaaggaaaag gcggttgcca
     4981 tgcaaaagcg gctgctggca aatcttcccg ccaaccatcc attgcgcagg aacgctgccg
     5041 aactttgaca tgtccaattc aacattcagg ccataatatc caagtccatg agcttcccgc
     5101 cacccactcc caaacaggcg cgtgtgctct ggtcctgcct gacgtccctg gcggtgacgc
     5161 tgcttctgct tttgggaggc cttttgatct ggggacttgg ctgggtgctg cacaaacttt
     5221 cggcaatttt ggtgccgatg attttcgcgc tgattctcgc ctacatcctc gacccggtgg
     5281 tggaattttt ccaacgcaag cgcctgccgc gcatttggtc cgtcagcctc gtgttcgctc
     5341 ttgccgcatt gctcttggtg gccgtgcttg gctcggtgtt gcccggcttg cggcgcgaga
     5401 gcaaaaaact catcgatgat ttgcccaaga ccagcgcgtt gatccgcacc aagatcgact
     5461 ccttcctgaa cgactccgcg ctcggcaaac aattgcctga ttcatggcgc gatcccttga
     5521 aatccgttgt cgcacagtct ccgacgaata aaagcgcgac gtcctccaac actcccgagt
     5581 ttttgccatc caccaacgca attcccgata caaccatcag cacgaattcc acggacttgg
     5641 cgaccggtgc gaaaacaacg gattccgggc aaatttccaa caagcccgtg ctgcccggat
     5701 tgaccggcgc gttggtcttt gtgacaaaat ggttttacgc gcaattgagc aaaatttcga
     5761 cctgggcgga atttctcatc ggcttcattc tcgtgccggt ttaccttttc tatttcctgc
     5821 tcgaaaaaaa atccatcacc agccgctggc cagattattt gccgctcaaa ccttcccgcg
     5881 caaaagacga aaccgttttc gtgctccggg ccatcaacga ttgcatgatc gtcttctttc
     5941 gcggacaagt gctggtcgcg ctttgtgtgg gcgtgctgct ggcggcgggt tactcgctga
     6001 tgggcttgaa ttatgccgtg ctgctgggat tggtcgccgc agtgctgggc atcgtgccgt
     6061 atctcggaac catcacgagt ctcttgctgg ctttgacggt ggcaggcgtg caattccagg
     6121 actggtcgca cccgtttgcc gtgcttgcca tcgcggcgct ggtgaaactg ctagaggatt
     6181 tcatcatcgg tccgtgcatc atcggtgaac gcgccgggct ccatccgctg acaatcattc
     6241 ttgcggtgct gatcggcacc acgctgctgg gcggattcat tggcgcgttg ctggcgattc
     6301 cgctcaccgc cgcgctgcgg acgttgatgt tccgttacat ctggatccat gaacatccac
     6361 gcgcgaaacc gtccgaagtt cgaaaagagt tggagccttt ggggaagtga tttgcgcgaa
     6421 ccagccaaat ccttccttgc gcggacgcgc ggcatttgtt aacaatgaaa gagtcaccaa
     6481 tgcgggtgtg gttcaatggt agaatgtggc cttcccaagg ctgagacgag ggttcgattc
     6541 ccttcacccg ctccattgat aataaaggtt tttctttttt tggttgaaag gtaaacgctg
     6601 gagttcaaca tttgttcgcg aaaaaaaagc gaggttgaat tttttttgga tttcttcggg
     6661 aacaaaactc acgtccgcca ctcataactt ccagatacac tgatccatca tgcgtcagga
     6721 cggcgagcgg aaagcttgaa cggaacagat caaattccat gaaatcaaaa accctaaaac
     6781 aatttgtgtt tatcggcatg gcaagtttat ttctaccgat ggtgacgcaa gccgccacca
     6841 tctggaatgg accgccggtc agtgagagcg atgccaccga gccggaccag atcacggctc
     6901 gtgtctggct gacgcgcgga agcagtgagg gactttacaa cgccgtgact gaaacggggt
     6961 tcacccattt cttcagcccc gccgacacgg aatgggccga tgggaccacc gccaactatg
     7021 cgtcgctttc ctacacggat tggaacacat gggccaaaaa tattcatggc ggcccgccga
     7081 acacggtcgg tgtgaacgct gtcgtgcatc tcatcacgga tgacatttat atagacatca
     7141 tgttcacgtc ctggagcact ggcggctctt attcttatca gcgagccacg ccgcctgcgg
     7201 ccgacgtgcc gcccactatc gccatcactt ccccgacgaa cggcgcatcc ttcgccgcgc
     7261 ccggcattgt ttccatcact gccagcgcga gcgactccga cggcagcgtc acgaatgtgg
     7321 cttttttcga cggagccacg ttcttgggcc aaactaacaa tacgccctat accgtgacgg
     7381 ccagcctagc cgccggttct cacgcgctta ccgcggtcgc cacggacaac gccggccttt
     7441 ccacgacctc cgccatcgta aatgtgactg tcagcgccgc caacgtgccg ccaaccgtga
     7501 ccatcaccaa tccgcctaac aacgccgttt tcggcaacac ggacactata accgttcaaa
     7561 cctccgcgag tgattcagat ggcaccgtca ccaatgtgca actttttaac ggcacgaatt
     7621 tgttgcggag ttttaccaat gcgccataca acttctccgg taccgcactc gcgggcaact
     7681 tcgccttggg cacaaatatc ttgaccgcag tcgctaaaga caacctcggc gctacaacca
     7741 cctcggcacc cgttcatgtg atcgtggccc gttacacagc ggaactcacc aacggggcct
     7801 tttccgtttt cctcataccc gttgccacca acttgtccgc gcccctctat ggcgtcagtc
     7861 cacccagtga cacccaccgc ctgttcgtgt tggagcagaa cggtttgatg tggatcatcc
     7921 aggacggcgt tctgctgccc gcgccggcgt tggatcttcg aagcctcgtc cagccaccgc
     7981 tcattgcaaa caacgccaat gacgaacgcg gcttgctcgg catggccttt catcccggat
     8041 tcaacaatcc agccagcctt ggttatcgaa cggtttatac ctacaacagc gaactgatac
     8101 cttccaacac cctgcccacc tacccgctgt caggcggaac atccaatctt tacaaaaacg
     8161 ctctcaacga gtggaagatc tcgatcacga acgccaacgt gattgatcca tccactcgcc
     8221 gggagatcat ctcctttggc aagaacgccc tgaatcataa cggcggcacc tgcaccttcg
     8281 ggccggatgg ctacatgtac cttgcgttgg gtgacggcgg caacgccaac gacgtcggac
     8341 ccggtcacat ctcacccggt ggcaacgccc agaacttgag cgtcccgctc ggcaaaatgt
     8401 tgcggttaaa tcccctcaac ccggcgctaa acccctcaag ttccgatcca atcagcagca
     8461 acggccaata ccgcatcccg gccagtaatc cgtttcaggg accgggccag gtgccggaaa
     8521 tttatgccta cggatttcgc aacccctatc gctttgactt cgaccccgtc acaggtgatt
     8581 tgatcgaagc tgacgtgggt cagaacaaca tcgaggagat caatcgcgtc gtcatggccg
     8641 gcaactatgg ctgggcgatc aaggaaggcg acttcctgtt caacatgacc aacggcaccg
     8701 tcgggactcc gccgggaaat ttcagtcccg gaattccaac gggactgatt gacccgatct
     8761 ccggcccgaa gggtattctg gaatacgacc acaacaacgg catttccatc accggtggat
     8821 tcgtctatcg cggcgccggg atgccggaac tctatggcaa gtatatcttt ggtgacctgg
     8881 ctctggtggc aaaccctgtg cgggagaatg gacgtctctt ctatgccgac cttcaacaag
     8941 gcacgataaa cgcctttacg ttgccgcagt ttggctcgga gattctgccg aacggcgaca
     9001 cagtgcacgg tttcggtcag gatggcagcg gcgaactcta cgccatggcc acagacaccc
     9061 cacccaatgg gaacggcggc gttgtttaca aactagcctc gctgcgcctg acgctccaag
     9121 agaatggcaa ccagttagac atctcctggc ccaccatcgc cggacacctg caagcgcaga
     9181 ccaataactc gaccggcggg cttggcacaa actgggtcaa cgtgccgggc tcggcagcca
     9241 ccaatcacgt cactgtttcc atcaatccat ccaacggcag tgtgttctac cgcctggtcg
     9301 tgccttgata gatgaatact cgccagcgcc acatcatcaa ggtccgatga cttttaaacg
     9361 gtagaagcgg gcttgagcat tcgtaaccgg gtcaatgaca gtttgtgtgg ctgcattgcc
     9421 ggtaattcca ctgatcaggg tatcccaatt tgtgttcgaa agatttgtgt tccactccac
     9481 gctgtaataa tggtttgtca gtgttaaaaa tgacagtgag agcccatttt ggaacgccac
     9541 cgcgtcaatt ctcggcggag gcggagggtt gatggcaagt gccaaaaccg cattgccggt
     9601 tccattccga ttgatggcgc gaagcgtgac cgcgaaactc cctgtcacgg caggcacgcc
     9661 aaagatgcgt cccccagccg gatcgaacga aagtcccgct ggaagaccaa tcacaaagaa
     9721 gctggtggga gtgttcgatg cggtaatctg gtaactgaag ctcgccgcca catttcccat
     9781 cgcagtcaaa acgctggtaa tccccggcgc gggagcggca ccgttataaa cgaccatggt
     9841 cattgaattg gtggtcgtgc cagtggaatt ggcggcggtt atggtaacgt tgaaagtgcc
     9901 ggaggtcgtg tttgtgccag tgattgcacc ggtgagggaa ttcaacgtga gtcccggagg
     9961 caaaccagtc gcgctgaagc ttgttgccac actggtcgcc tgaatcgtgt aattgaaggg
    10021 tgaatttacc aggccatcgg cagcggcgga actggtgatg acggacaaag gcaatttcac
    10081 ggtcaaggca agattgccat aaccggcgcc gctcgcattg gtggcggcaa gttgaatatt
    10141 ataggtgccc accatggctg gtcttccgga gatcacattg ttggtgaacg tcaatcctgg
    10201 cggcaagttc atcgcgctga atccggtcgg gctgaagctc gccgtaatat tgtaagtgaa
    10261 cggcattcca acgatgatcg acgccgaggc cgcgctgttg atcaccggag cattcggcgc
    10321 agggctgacg gaaatcgttt tgtcttgagt cgtcatgtgc aaatcatcaa acgcgcgcaa
    10381 ggtaaaagta taactcccgg gaatcaggag attgccaacg gcggtattgg tggcgccctg
    10441 atgatctaat tgcggttcag cgccaggggg aaccgttttt atcgcccacc aatggcgtag
    10501 cggttgattg gttgagccgc tggtgacggc catgagttgc aaagaattcg ccggcagggt
    10561 cacgcccggc ggggaggccg tgatggaact gatgacaggc ggtgaagtcg cggtgtgaac
    10621 tgtgcagaga atctgcgccg taagatcgga atgcccggga ttggtcacat tgatttgaaa
    10681 caggtaatct ccgggtaccg tcatgttcgc gacattggcg cggaggctca caaaaatata
    10741 ggtcgtggcg ctcacgcctg cgttcgctcc cgcaggctgg ctcacgagac tccatgtgcc
    10801 gcgtccggtg aagtcgctgt ttgcgagatc ggaaattccg gcctgcaaaa ctgcggacga
    10861 agtcggcagc tcaatggtgg catgagtcat gccgccggga tcgccgaaaa ccaaaccgta
    10921 aggcgcggca attctaaatc cggtctggcc caggaccggc ggcggattgg aaccatagac
    10981 ctccaggtac aactgcctgc tagaaacatt tgttccgtcg ctcacatccg cgttgaaaat
    11041 gtaattgccg gcggcggtca acccgttgac gacagtggtg ggcgcgtttg aatttgcaaa
    11101 tacagcattt gcgcccgccg gctgattggt caccgaccaa tgatacgtca gcgtggccag
    11161 ttcgggatcg ctggccatga tggaaagcgt tgcggaattt gtcggcgcga ccaggtatcc
    11221 cgggcttgcg ctccaaactt caattgtcgg cggctggtta gtgccggcac ggggatacaa
    11281 agttgtgact gccgaattac ccgccacatt ggtcagtgtg aggagaccgt tcgtcccaat
    11341 tgtgcgcaca ccgagttcct gaaaagaact ggcgcccgag accgcctggg acaaaccata
    11401 gcttcctggc ggcagcgcgc tcaaattgat tgtttgagcg gaaggcgacg tgttctcaat
    11461 gacggtggtc actttgccat tttgcgaaaa agcaagaaca cgaagcgacg gatcactcga
    11521 tgccgcgccg atccgaactg cgcccggctg gacgtagtgc atcagttggc ggagacgaaa
    11581 aaaagtgcct gacggagtga aagccgtcag tcccgcgtta ttggcgagtg tattgccgcc
    11641 actataagcc acttcccaat aggtgacacc cgccagggtc aaatcattga acaaatcatc
    11701 gaaggtggga tttcccatct ccgtctgcgc ggtgcccagc cccctggcat ttccaaaatc
    11761 gcgcaggtag gaacgatacg gatcggctgt gccataattg tgatatgaaa ttcgcccgac
    11821 gtatttccag aggtcaggat cactcaatgc cggcgtgatg taattccaat ccgtttgggg
    11881 cgcgactgct tccgcatatt gaacccgagt cgtgagacct tgagcaagga accgtgggcc
    11941 aagcgccttt atgtcgtcgg tcaggattgt ataggccatg cttggttcgt tgtaaatcac
    12001 cgcataactg atgttgagtc cgtaattggt ctttaaataa agcgaactgg cccaaatttg
    12061 ctgtgcgcgt tcgcccggat ggttcatcac ccagggcttg gaatcagaag catgagtcgg
    12121 ataacctggg cttgaataaa aggacggttg aaaaccttcg gcgagaattc gattttgata
    12181 atggaccagg taggcggcat cttgagcgct gaacgtgtct gcctcgaata aattccaatc
    12241 aacaaagtcg caatcaccgt cgtcagtgcc ggttccatca atgcggggac cgacttccca
    12301 cgtcctcgtt ccagtcagcc ccaaatcatc agtcagatag tccagatatt gataattgac
    12361 cgggtcggcg accgcctgtg gcaacatgct ttcggctcca gccgtgccgc caagaacccc
    12421 gcccccatgg ccccatcccg tgatggtttg aaatgtttgg gccggttgaa tgactacatc
    12481 agcgcaagac ccgggacaac aagatgcaat caatccggtg agaacaagcc caaaaaacac
    12541 cacgccatcc gatgcttttg ccataatgca gggcctttgg ctcatgaccg ctctagcctc
    12601 gcccattgct cctctagctt caagtgaatc attacgagcc gacatgtgga tttttaacct
    12661 ttttagccat gcaaccactc ccaatgtgtc acttgatttc gcctcgcata tcaagttccc
    12721 acatccaaaa tcaatccggg tcatcgcgca gaaatcgcat tgattgcttt cttgagccgt
    12781 ccctcacttt atcttgaaaa cttcagctca tgtggcatga ttatgccctg cattatatga
    12841 gctgcttttg cggacaactg ttgctgtacc gcgtttcctt tctcaaggga tgtgttcttg
    12901 tcgcaaggga tggaggacga cgcaagactt caccaatcac aacccaatca acctatgaaa
    12961 gcgaaccaac agatcaaaca aatcatcccg gctggcatca ggacggcctt tgtgggcctt
    13021 ttcttcgttc tctggttttg cgctgacgtt ttggcgcaag acgaatcctc attcaataca
    13081 cccggaggcg tgcattacaa tgctgacggg accctctcgc tgcaatggac cggccccgga
    13141 actcttgagt ccgccccggg gcttgcgggt ccatggactg cggtttcgag cggctcatcc
    13201 tatgcgggaa acgcgaccgt tccggcgagc ggaactgcgg gcttttttcg tcttcactcc
    13261 gggtctcgga ttggagctgt tttccccgcg cctgtcatcc cggcattgcc cgcggttcaa
    13321 tccgccacca ttcaacgatt aaatt
//
LOCUS       DASZMM010000083         6572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1123576, whole genome shotgun sequence.
ACCESSION   DASZMM010000083 DASZMM010000000
VERSION     DASZMM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123576"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VG754_02155"
     CDS             complement(<1..371)
                     /locus_tag="VG754_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18036.1"
                     /translation="MKFSILIASYLSKDTWKRWCEQPGSPLARWFVTGLLVMVATVIL
                     VAFQLLERGLQDRLARFGLDTLLVRESVTPGSLAFFRQGDGPDSLGVLASNGKKLRLR
                     QLFVRGRTEWQQEDLYVFSYPA"
     gene            complement(310..966)
                     /locus_tag="VG754_02160"
     CDS             complement(310..966)
                     /locus_tag="VG754_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF18037.1"
                     /translation="MRCLELSFRYCRERPWVIGDFNYTFAPGITLIKGASGCGKSTLL
                     RLLAGFLKPEQGKIITPLGSAPTDAEFQRRDLGFVFQQLNLLPLATVQRNLALAASIA
                     GLQDESIAENSTRWLGLLGLEPYVKRLPHSLSGGQQQRAAIARALVKEPRVLLLDEPT
                     SGLDDLNTRVITRALEHFVTGERICVISSHDARLEHLANEILDFNRFLPLERHLETLV
                     "
     gene            complement(969..2279)
                     /locus_tag="VG754_02165"
     CDS             complement(969..2279)
                     /locus_tag="VG754_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18038.1"
                     /translation="MSQPLKNTGEYELGWAGRIANSWITVVIYLWCVLLMWLFVTGCS
                     RKPTAPPTPSATNAWSWEPYPAAEKMRLATLPCQVLPRTSLTISAPVSGQLRLYVDRA
                     QTNLPAGFLWAEFEPKALNLEAAELAEARRRIVEREKLFEKIELPKEVIKLNKDISAM
                     KKQVMLLEMLSTNAELARAAINAAGLDRDGALKAGSLEDARKELALMEDNLKFLAGTN
                     SAVIGFDIEGARMELERRQLDFDRRRAQSRFTMPFAGQWISSLQLADGVMEYPVSAGQ
                     ELAVVRDLHSILLRVPLEDVSWSGLPTDKLSAVVPLADGTHLEAAFAFKKLEKFQLRE
                     EVFYYFQFPFEDSAAAALLVGTSPTCDLWLELSQPARIVPKLALVLHQPDAFQNRRWN
                     EGLSQLFPGSQLLVEGQTELAILPPVNEISSNLQRSKLTRADPP"
     gene            complement(2264..3595)
                     /locus_tag="VG754_02170"
     CDS             complement(2264..3595)
                     /locus_tag="VG754_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18039.1"
                     /translation="MSLKPPLPIWLQFSCLGLLILPGCVTPQKRIAREMPALRSQWQA
                     DVAHQSALPERRVSWLEALALLEKNNLKLRGARVDITNSEEIARQVYRDLIPTVNLRS
                     GVTKSVKNLPATSFDDVTFNIDSFFNIPGVVNFNARLFSGRLSVMRAKAACQIAEREQ
                     TIELYKLFLEARENDEQAAELKTERSVAEAVRKADEISGEVLLKDVIAQEALLAKTDE
                     SFQTRIGDLLAERRFHWVLSTNGFPEFDYAAHPLPLEDTNRVAQLQMKMVALELVGDW
                     AQVHGIKLQYWPELSFFVTGPSAFQFANGQGNFASAANVVGSADFFWSLDTRGYVSQQ
                     LRTTRRAQDLEKAQLQMDALALIDQLIAARKTEVSLREQAVQLDRLLAFLDKVPPDVD
                     VNSILQRAETNRSLHFQRARLRRDMAELNTLFWFVDEQKWAEQPKELCLNH"
     gene            complement(3751..4218)
                     /locus_tag="VG754_02175"
     CDS             complement(3751..4218)
                     /locus_tag="VG754_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18040.1"
                     /translation="MKYNKSIALTTCLVSTALLTGCPGGSSNSNNNNNNNNNGTAPGS
                     LQGLTINVTVTGGNKPFSSSGSYTFTPTSGNGNSGTYSLQGQGGVQSNVGSYTYSANG
                     NTGTLTETEQSGTLVNNTLTFQTSNSGTIYSSSPPGTGPQDVGGNQTGNFTIN"
     gene            4466..5971
                     /gene="carB"
                     /locus_tag="VG754_02180"
     CDS             4466..5971
                     /gene="carB"
                     /locus_tag="VG754_02180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWF18041.1"
                     /translation="MPKRTDIHSVLIIGAGPIIIGQACEFDYSGTQACKALREEGYRV
                     VLVNSNPATIMTDPEFADRTYIEPITPDCVEKIIIRELAEMKRLGAKGKLVLLPTLGG
                     QTALNTAMALHRRGTLERHGVEMIGANARAIERGEDRQIFKDLMIKIGLDVPISGTAH
                     NVEEARAVAEKIGRFPLIIRPAYTLGGTGGGIAYNREEFDEIAKRGIELSPVSEILVE
                     ESLLGWKEYEMEVMRDRANNCVIICSIENFDPMGVHTGDSITVAPIQTLTDKEYQIMR
                     DASFAVIREIGVETGGSNIQFGVNPDNGRMVIIEMNPRVSRSSALASKATGFPIAKIA
                     AKLAVGYLLDEIKNDITRETPASFEPTIDYVVTKIPRFAFEKFPQADPTLNTQMKSVG
                     EAMAIGRTFKESLQKCLRSLEIGRSGLGGDGKPWRIGEDVYGDRDILPREIITRKLST
                     PNAERIFFIRHAMRAGFTLDEIFNLTKIDRWFLTQIKEIVDFEEELAMIAR"
     gene            complement(5968..6378)
                     /locus_tag="VG754_02185"
     CDS             complement(5968..6378)
                     /locus_tag="VG754_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWF18042.1"
                     /translation="MKKAKSGNQKSVAKGARAPKDVDEYIAATPEPARSILKKIRAVI
                     RAAVPPEASEIISYNMPAFRHKGVLVWYAAFSDHCSLFPTSAMIEMFKKELKGFSTSK
                     GTIQFPLDKPPPIGLIKKIVKARVAQNETKRAKS"
     gene            6496..>6572
                     /locus_tag="VG754_02190"
     CDS             6496..>6572
                     /locus_tag="VG754_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWF18043.1"
                     /translation="MKGIILAGGAGSRLYPLTLVASKQL"
BASE COUNT         1461 a         1950 c         1718 g         1443 t
ORIGIN      
        1 gccggataac tgaagacgta aagatcttcc tgctgccatt ccgtccggcc gcgaacgaac
       61 aactggcgca accgtaattt ttttccgttc gacgccagca cgccaagcga atccggacca
      121 tcgccttgcc ggaaaaacgc aagcgaaccg ggcgtcacgc tttcgcgcac gagcaatgtg
      181 tcgaggccaa agcgcgccaa acggtcctgc aagccgcgtt ccagcaattg aaatgcgacg
      241 aggatgactg tcgccaccat cacgagcaac ccggtgacga accagcgcgc gagcggactg
      301 cccggctgtt cacaccagcg tttccaggtg tctttcgaga ggtaggaagc gattaaaatc
      361 gagaatttca ttggccagat gttcaagtcg ggcatcgtgc gaactgatga cgcaaatgcg
      421 ttcaccggtc acaaaatgtt ccagcgcgcg ggtgatgacg cgcgtgttca ggtcgtcgag
      481 gccggaagtc ggctcgtcca aaagaagcac gcgcggttcc tttaccagcg cgcgggcaat
      541 tgcagcgcgt tgttgttgcc cgcccgaaag cgagtgcggc aaccgtttca cgtacggctc
      601 caatccaagt aaaccgagcc agcgggtgga attttccgca atgctttcat cttgcaaacc
      661 ggcaatgctc gctgccaagg ccaggttgcg ttgcacagtc gcaagcggca gcaggttcaa
      721 ttgttgaaag acaaatccca aatcgcgccg ctggaattca gcgtccgttg gcgcggagcc
      781 aagcggcgtg atgattttcc cttgctccgg tttcaaaaat cctgccagca atcgcaataa
      841 cgtggacttg ccgcaacccg acgcgccttt gatcaacgtg atgcccggag cgaaagtgta
      901 attgaaatcg ccgatgaccc atggccgctc gcgacaataa cgaaacgaaa gttcgaggca
      961 acgcatggct aaggcgggtc tgcgcgggta agtttggaac gttgcaggtt cgagctgatt
     1021 tcattcaccg gtggcaggat ggcgagttcc gtctggcctt ccacaagcaa ttgcgaaccg
     1081 ggaaacaact gtgagagccc ctcattccat cgccgatttt ggaacgcatc cggctggtgc
     1141 aacaccagcg cgagcttggg cacgatgcgc gcgggctgcg aaagttcaag ccagagatcg
     1201 caggtcggcg atgtccccac caagagcgcc gccgctgcgg aatcttcaaa tggaaattgg
     1261 aaataataaa aaacctcctc gcgcagttgg aacttttcca atttcttaaa tgcgaatgcc
     1321 gcctcaagat gcgtgccgtc tgccagcgga acgactgcgc tcaatttgtc cgttggcagt
     1381 cccgaccacg aaacgtcctc caatggcact cgcagcaaaa tcgaatgcag gtcgcgcacc
     1441 actgcaagtt cctggcccgc gctgacggga tattccatca cgccgtccgc cagttgcagg
     1501 ctggaaatcc attgtcccgc gaatggcatg gtgaaacgcg attgcgcccg acgccggtca
     1561 aaatccaatt gtcgccgctc cagttccatg cgcgcgcctt cgatgtcgaa accgatgacc
     1621 gccgaatttg tgcccgcaag gaacttcaaa ttgtcctcca tcaacgcgag ttccttgcgc
     1681 gcgtcttcga gcgacccggc cttcaacgcg ccgtcgcgat ccagcccggc ggcattgatc
     1741 gcggcgcggg ccaattccgc gtttgtggag agcatctcca gcagcatgac ttgttttttc
     1801 atcgccgaaa tgtccttgtt cagcttaata acttcctttg gcaattcgat tttctcgaac
     1861 aacttttcgc gctcaacgat gcggcggcgc gcctcggcca attcggccgc ttcgagattc
     1921 aacgccttcg gctcgaattc ggcccaaaga aatcccgccg gcaaattcgt ctgcgcccgg
     1981 tcaacataaa gccgtaattg gccggacact ggcgcgctga tcgtcaaact ggttcgcggc
     2041 aaaacctggc aaggcagcgt cgccagccgc atcttttccg ccgcaggata cggttcccag
     2101 gaccatgcat tggtcgccga aggagttgga ggcgcggtgg gtttcctcga acaacccgtg
     2161 acgaacagcc acatcaacaa aacgcaccaa aggtaaatga ccactgtgat ccaggaattg
     2221 gcgatgcgcc cagcccagcc taattcatat tcacctgtgt tttttagtgg ttgagacata
     2281 attcttttgg ctgttcagcc cacttttgtt catccacaaa ccagaaaagc gtgttcaatt
     2341 ccgccatgtc gcggcgcaac cgggcgcgtt gaaaatgcag cgaacgattg gtctcggcgc
     2401 gttgcaaaat ggagttcacg tccacgtccg gcgggacctt gtcgaggaag gccagcaacc
     2461 ggtcgagttg cacggcttgt tcacgcagcg acacttccgt tttccgcgct gcaatcaact
     2521 ggtcaatcaa tgcaagcgcg tccatctgca attgcgcttt ctcaaggtcc tgcgcccgac
     2581 gcgtcgtgcg caattgctgg ctgacatacc cccgcgtatc caatgaccag aagaaatccg
     2641 ccgatccaac cacattcgcg gcggacgcga agttcccctg gccattggca aattgaaacg
     2701 ccgacgggcc cgtgacaaag aaactcagct cgggccaata ttgaagtttg atgccgtgga
     2761 cttgcgccca gtcgcccacc aattccaacg cgaccatttt catctgcaat tgcgcgacac
     2821 ggtttgtatc ttccagcggc aaaggatgcg cggcgtagtc aaattccgga aaaccgttcg
     2881 tgctcaggac ccaatgaaac cgccgttccg caagcaaatc gccgatgcgc gtctggaaac
     2941 tttcatccgt tttcgccagc aatgcttcct gcgcgatgac atctttcagc aacacttcgc
     3001 ccgaaatctc gtcggccttt cgcaccgctt ccgcgacgct tcgttccgtt ttcaactccg
     3061 cggcctgttc gtcgttttcg cgcgcttcca aaaaaagttt gtaaagttca atggtctgtt
     3121 cgcgctcggc aatctggcag gccgccttgg cccgcatcac cgacagccgc ccggagaaaa
     3181 gacgcgcgtt gaaattgacc acgccgggaa tgttgaaaaa actgtcgatg ttgaacgtga
     3241 catcgtcaaa ggaagtggcg ggaaggttct tcacgctttt cgtcacgccg gaacgaagat
     3301 tgaccgtggg tatcaggtcg cgatatacct gccgggcaat ctcctcgctg ttggtgatgt
     3361 ccacccgcgc cccgcgaagt ttgagattgt tcttctccag caacgcgagc gcttccagcc
     3421 agctcacgcg acgctccggc aatgcggatt ggtgcgcgac gtccgcctgc cattgcgagc
     3481 gcaaggcggg catttcccgc gcgatccgtt tttgcggggt gacacaaccc ggcaatatca
     3541 gcagcccgag gcaactgaac tgaagccaga ttggcagtgg cggtttgagt gacacatttt
     3601 aaaaatacag acgccgtctc ccaatcgttt cgcggcatgg gcagaatacc gcgttcctcc
     3661 ccctcgcccc catcgcggga gaagaatgtt ttggaacgac ggcgtcatga tttggtttgc
     3721 gaccggcaag cagccggacg cgaataatca ttaattgatc gtaaaattac ctgtttgatt
     3781 tccaccgaca tcctgcggtc ccgtgccggg cgggctgctg ctgtagatgg tcccagagtt
     3841 gctggtttga aaagtgagcg tgttgttgac caaggtgccg gattgctccg tctcggtcaa
     3901 agtgcccgtg ttcccgtttg cagaataagt gtaggagccc acgttacttt gcactccacc
     3961 ctgcccttgc aacgaatacg tgcctgaatt accgtttcca ctcgtgggcg tgaaggtata
     4021 gcttccagag ctggaaaatg gcttgttacc gcccgtcacc gtcacgttga tcgtcaatcc
     4081 ctgtaacgag cctggcgccg tgccattgtt gttattgtta ttattattgt tgctgttgct
     4141 cgaaccgcct ggacaccctg tgagcaaggc cgtcgaaacc aagcacgtgg tcagcgcgat
     4201 tgatttattg tatttcataa ccgctgatgt ctctttctgt tagttgatta ttcaaaaaca
     4261 gcagcagtat gcaccgcata ccgtaggata gaatagggtc ttgcacctac tgtcccgagg
     4321 aagccacccc taaggcgcta ccctacacgt ttgagacgcc ctccaggaat tttcttgatt
     4381 tggcctgtga aaattggccg gcgtcgcagt ttgcaaattt aaaccatgac aactgcccca
     4441 gaatcgtttt aattctcacg gttttatgcc gaagcgtact gacattcatt ctgttctcat
     4501 catcggcgcc gggcccatca tcatcggcca ggcctgcgaa ttcgattatt cgggcacaca
     4561 agcgtgcaaa gccttgcgcg aagaaggcta tcgcgtcgtc ctcgtgaact ccaatcccgc
     4621 caccatcatg acagacccgg agttcgccga tcgcacttac atcgagccca tcacgcccga
     4681 ttgcgtcgaa aaaattatca tccgcgaact cgcagaaatg aaacgtctcg gcgcgaaagg
     4741 aaaactggtg ctgctcccca cgcttggcgg gcagacggca ttgaacaccg ccatggcgct
     4801 tcatcgccgc ggcacgcttg aacggcacgg cgtcgaaatg atcggcgcga acgcccgcgc
     4861 catagaacgc ggcgaagacc gccagatttt caaggacttg atgatcaaaa tcggcctcga
     4921 tgttcccatc agcggcaccg cgcacaacgt cgaggaagcc cgcgccgttg cagaaaaaat
     4981 cggacgtttc ccgctcatca tccggcccgc ttacaccctt ggcggcaccg gcggcggcat
     5041 cgcttacaat cgcgaggaat tcgacgagat tgccaaacgc ggcatcgaac tttcccctgt
     5101 ttccgaaatc cttgtcgagg aatcgcttct cggctggaag gaatacgaga tggaagtcat
     5161 gcgcgaccgc gccaacaatt gcgtcatcat ctgctccatc gagaacttcg accccatggg
     5221 cgtgcatacc ggcgattcca tcacggtcgc gcccatccag acgctcaccg acaaggaata
     5281 ccaaatcatg cgcgacgctt cgttcgccgt gatccgcgaa attggcgtcg agaccggcgg
     5341 ctcgaacatc cagttcggcg tcaaccccga caacggccgc atggtcatca tcgagatgaa
     5401 cccgcgcgtg tcgcgttcct ccgcgctcgc gtccaaggcc accggctttc ccattgcgaa
     5461 aatcgccgcc aagcttgccg tcggttatct gctcgacgaa ataaaaaacg acatcacgcg
     5521 cgaaacaccc gcgagcttcg agccgacgat tgattacgtc gtcaccaaaa ttccgcggtt
     5581 cgcctttgaa aaatttccgc aagccgatcc cacgctcaac acgcagatga aatccgtcgg
     5641 cgaagccatg gccatcgggc gcaccttcaa ggaatcgttg caaaaatgtt tgcgctcgct
     5701 ggagattggc cgcagcggct tgggcggcga cggcaagcct tggcgcatcg gcgaggacgt
     5761 gtatggcgac cgcgacattt tgccgcgtga aatcatcacc cgcaaactca gcaccccgaa
     5821 cgccgagcgc atcttcttca tccgccacgc tatgcgcgcc ggtttcaccc tcgacgaaat
     5881 ttttaacctt accaaaattg accgctggtt cctgacgcag ataaaagaga ttgtggattt
     5941 tgaggaagag ttggcgatga tcgcgcgtta acttttcgcg cgcttggttt cattctgggc
     6001 gacacgcgct ttcacgattt tcttgatgag tccaattggc ggcggtttgt ccaacggaaa
     6061 ttgaatggtc ccttttgatg tcgagaatcc cttcagctcc ttcttgaaca tttcaatcat
     6121 cgccgaagtc gggaacaagc tgcagtgatc cgaaaacgcg gcgtaccaca ccagcacacc
     6181 tttgtgcctg aacgccggca tgttataact gataatctcg ctggcttccg gcgggacggc
     6241 agcgcggatt accgcgcgaa tcttcttcaa gatgctgcgg gcaggttccg gagttgcggc
     6301 aatatattca tcaacatctt tcggagcgcg tgcgcctttc gccacagact tttgattgcc
     6361 cgattttgcc tttttcatgc gaataatcgt tacaccaggc tcgaatttga atcaatgccg
     6421 cggcatttcc gcgcttggga aaactcatca acttgtcctt ttcttcccgt cggctttctt
     6481 ttacatttcg tttcgatgaa aggcatcata cttgccgggg gtgccggctc gcggttgtat
     6541 ccgctgacgc ttgtcgccag caaacaactc ca
//
LOCUS       DASZMM010000084         7444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1947872, whole genome shotgun sequence.
ACCESSION   DASZMM010000084 DASZMM010000000
VERSION     DASZMM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947872"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>7444
                     /locus_tag="VG754_02195"
     CDS             <1..>7444
                     /locus_tag="VG754_02195"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWF18044.1"
                     /translation="FIQVMFVLQNPPGEGTKLGDLQLTPVDVPLDVAKFDLTLFIEER
                     GKTGVATLEYNTDLFDAATISRMLGHFEQLLKGIVANPARRLSDLPLLTDAERKQLLV
                     EWNQTQTDYPRDRSVHELFEEQARMRPESPVVIFGESCLTYSELDKRANQLAHRLARS
                     GVCAGAVVGICLERSVEMIVALLGILKAGAAYASLDPASPKDRIAMMLEDLKTPVVLI
                     QEKFQSLFPNTASGQTEPNLICLDSDWQSIARENDESPKCNISATDLAYVSFTSGSTG
                     RPKGVCVPHRAVVRLAKNTNFATFSPDEVFLQMAPISFDASTLEIWCCFLNGGRLVVM
                     PPEIPSLSDLSRVIQKHQVTTAWFTSGLFNQLVDEEPEALKSLRQILAGGDVLSSSHV
                     KKVMEWPGARRLINGYGPTENTTFTTCYTVPVDFTGDRSVPIGRPIANTQCYILDAHL
                     QPVPIGVTGELYTGGDGLATGYINQPGLTAEKFIPNPFKPGECLYKTGDLARYLPDGN
                     IEYLGRADGQVKLRGFRIEPGEIETILNAHPEVRESVVIATGESANEKRLAAYFVSRK
                     QSPPTAAELREFLRTKLPEYMLPSRFIPMEQLPLTQNGKVDRRALLAQPETRSAPEKK
                     LSPPKDALEAELKEIWESVLGINPIGVEEKFFELGGHSLLAVRLVARLEKKFGRKLPL
                     SMVFQAQTIAQMAQYLRSEPGSGSVSSVVAIQSGGTKPPLFFVHGVGGGMFWGYTNLA
                     RSLGPEQPVYVLKSRAMDGQEEFSSIEQMAAQYVSDIRSMQPKGPYQLGGFCFGGIVA
                     YEMARQFEAAGEQVAMLTVLNSAPPNSNYWHFQWTPASMFRFARNLGLLIMRSLRWDP
                     QQRKEFLRWKAAQTRRKFSRMLKMPNGGSYQFSAEEVVDLSSFTRDQRKLWEAHIISL
                     IQFFPKRYKGKVTLFRSRGHPLLCSYDGQCGWDELASQVEMHVVPGEHESILEEPHVR
                     VLAAKLKECLRRLQSPETVPASVAGLKTKAADSQTGASANQPSADYPRELCVHQLFEE
                     QVKRTPDAPALLAGGERLTYAEINRRANQLAHHLEALGVGPDVPVGISLERSPELVIG
                     LLAILKAGGAYVPLDPAYPRERLSKMLENSRAPILLTQHQFVSTLPAENLQVILLDEP
                     LPAGLKESNPVPRASANNLAYVIYTSGSTGQPKGVAMEHRPLVNLLWWQLKNSAMGKG
                     DRTLQFASVSFDVSFQEIFSTWCSGGELVLISEEVRHDPQQLARLLAEEKVKRLFLPF
                     IALNHLSESIGAEESRSFSLREVITAGEQLRITNKITAFFEALPECTLHNHYGPSESH
                     VVTAYTMSGPSAKWAALPPIGQPIANTQIHLLDANLKPVLRDEPGELYIGGDCLARGY
                     LHRPDLTAERFVNDPFSAEPGARMYKTGDLARRLPDGNVEFLGRVDHQVKIRGFRVEL
                     SEIEAVLGKHPGVRECAVAAREDVPSQRRLVAYIVRQPAHVLTIDELRAAVLDKLPDY
                     MAPSAFVFLEALPLTPSGKVNRLALPAPDQNRPQLGGEFVAAANETEARLAGIWSEVL
                     NVKEVGTQDNFFDLGGNSLSAAQVISRVREAFKVELPVSCLFEAPTIASLAEGISTRR
                     WSEGRPAVPPLKRAPRSGNPPLSFTQGRLWFIDRLEPGSHAYNVPMAIRLQGGLDLKV
                     LQNSLDKIVSRHESLRTSVSFANGNLAQVISPDAHLKISVVNCETFPPSERAAQAKKA
                     VEKQARQPFNLERGPLVRCTLVRLSEQEHILLVVMHHIISDGWSLAVFFKELNLLYDA
                     YASGRLAPILPVLPVQYADYTLWQQQWMQGTVLDQHVSHWTEKLKGAPLSLQLPMDPP
                     SQTAPAGNSGQETLLLPKASSEALQGLCRREGLTPFMVLLAGLAITLQRWTEQSDMVI
                     GTVVAGRNHRELENLIGCFMNFVPLRITATETATGAEFLARVKSAVIDANAHQECPFE
                     KIVEAVNPERKTSQNPLYNVAFLMQNYPTATVGGRLLESEPVPVDLQAALLDLRFIAE
                     ESAAGISVACEYKADLFTAGTIEQLMASFTGVLAALVEEPGKQINQFQLVPQLAAQCK
                     AAREREDRQTIAVAATFTAEPVEDSLKYWMKELDMVGRVEFAPYNQVFQQLLDPSSLL
                     GTNQRGLNVIFIRFEDWEKTEGGPETPAVGDPSALERSIGEFITSLKSLIGRGAAPCL
                     ICLCPSSKSALLDSDRAALHRRMEELLSISLEPIAGAYVVSTKEINQLYPVAECHDAR
                     AEAFGAIPYTATFFTALGTVVARKFHALKRPAYKVIVLDCDQTLWSGVCGEDGPTGIE
                     LNPSRRVLQEFMRAQSASGMLLCICSKNNQADVAAVFESRREMPLKREHFAAWRVNWQ
                     PKSENLKSLASELRVGLDSFIFVDDNPVECAEVAANCPEVLTLQLPEPPEEIPQFLNH
                     CWAFDHLKLSAEDRQRTASYQQEKEREQLRSQSLNLSDFLAGLDLKVEIARLSSEQLT
                     R"
BASE COUNT         1634 a         2019 c         2140 g         1651 t
ORIGIN      
        1 cttcatccag gtgatgttcg tgctccaaaa tccgccgggc gaagggacga aactgggcga
       61 tcttcaattg acccctgttg atgtgccgct ggatgtggcg aaattcgatt taacattgtt
      121 catcgaagaa cgcggaaaga cgggcgtcgc cacgctggaa tataatactg acttgttcga
      181 tgctgccaca atttcgagga tgctcggcca cttcgaacaa ttattgaaag gaattgtcgc
      241 caaccccgcc cgaagattgt ccgacttgcc gctgttgacc gacgccgaac ggaaacaact
      301 cctggtagag tggaaccaaa cccaaactga ttatccgcgc gaccgctccg tgcacgaact
      361 gttcgaagag caagcgcgga tgcgtcccga atcgccagta gttatttttg gcgagagttg
      421 cttgacttac tcggagctgg acaaacgagc gaatcaactg gcgcatcgtc tggcgcgctc
      481 aggcgtatgt gcgggtgctg tcgtgggtat ttgtttggaa cgatcagtgg aaatgatcgt
      541 tgcgctgctg ggcattttga aagccggtgc tgcttacgcg tcgctggatc cggcctcacc
      601 gaaagaccgg attgcgatga tgctcgaaga tttaaagacg ccagtcgttt taatccagga
      661 aaaattccaa tcgttatttc ccaacactgc atccggccaa acggaaccca atttgatctg
      721 tctcgattcg gattggcaat ccatcgctcg cgaaaacgat gaatcgccta aatgcaacat
      781 ttctgcgact gacctggcgt atgtaagttt cacgtccggt tcaaccggaa ggccaaaagg
      841 cgtttgcgtg ccgcatcggg ccgttgtgcg gctcgcgaaa aatacgaatt tcgccacgtt
      901 ttcgccagat gaagttttct tgcagatggc cccaatttcc ttcgacgctt caacgctgga
      961 aatctggtgc tgctttttga acggggggcg cttggtggtg atgccgcccg aaattccgtc
     1021 gctctccgat ttgagcagag taattcagaa gcatcaggtc acgaccgcgt ggttcacgag
     1081 cgggcttttc aatcaactgg tggatgaaga gccggaggct ttgaagtcgc tgcgtcaaat
     1141 ccttgccgga ggcgatgttc tttcgtcgtc gcacgtcaaa aaagtcatgg agtggccggg
     1201 cgcccgacga ctcatcaatg gttacgggcc caccgagaac acgacattca cgacctgtta
     1261 cacggttccg gtggatttta caggcgacag gtcggtgccg attggacggc ccatcgccaa
     1321 cacgcaatgt tacatcctgg atgcgcattt gcagccggtg cccattggtg tgaccggcga
     1381 gctttacacg ggaggcgacg ggctcgccac cggctacatc aaccaacccg gattgaccgc
     1441 cgaaaaattc attccaaatc ctttcaagcc cggagaatgc ctttacaaga cgggcgattt
     1501 ggcccgttac ctgcccgatg gaaacatcga atatctcgga cgtgccgacg ggcaggtgaa
     1561 gctacgcgga tttcggattg agcccgggga aatcgaaacc attttgaacg cgcatcctga
     1621 agtgcgggaa tccgtcgtga tcgcgacggg cgaaagcgcg aatgaaaagc ggctcgcggc
     1681 atatttcgtc tcacgcaaac aatccccgcc gaccgcggct gagcttcgtg aatttctgcg
     1741 gaccaagctc cctgaataca tgctgccttc cagattcatt ccaatggaac aattaccctt
     1801 gactcaaaac gggaaagtgg atcgccgtgc gttgctggcg caaccggaaa cccggtcagc
     1861 cccggaaaaa aaactttctc cccccaagga cgccttggaa gcggaattaa aggagatttg
     1921 ggaatccgtt ttggggataa atcccatcgg agtcgaagaa aaattcttcg aactgggtgg
     1981 acattctttg cttgctgtgc ggctggtggc gcgattggaa aagaaatttg ggcgcaaact
     2041 tccgctttcc atggtttttc aggcgcaaac tattgcgcag atggcccaat acctgcgctc
     2101 cgagccggga agcggttccg tttcctccgt ggtggcaatc cagtcgggtg gcaccaagcc
     2161 gccgttgttc tttgtgcacg gcgtcggcgg cggcatgttt tggggttaca cgaatcttgc
     2221 gcggagcctc ggaccggagc aaccggtcta tgtattgaaa tcgcgcgcga tggacggaca
     2281 ggaagagttc tcgtctatag aacaaatggc ggcacaatac gtttccgaca ttcgatcaat
     2341 gcaacccaag gggccgtatc aattgggcgg attctgtttc ggcggcatcg tggcctatga
     2401 aatggcgcgg caatttgaag cggcgggcga acaagtcgcg atgctcacag tcctgaattc
     2461 cgccccgccc aattcgaatt actggcactt ccaatggaca cccgcttcga tgttcaggtt
     2521 cgcgcggaat cttgggcttc tcatcatgcg ctcgttgcgt tgggacccgc agcaacggaa
     2581 ggaatttcta cgctggaagg ccgcgcaaac acgccggaaa ttcagccgga tgctcaagat
     2641 gccgaacggc gggtcgtatc aattcagcgc cgaggaggtc gtggacctgt cgtcattcac
     2701 gcgcgaccag cgcaaacttt gggaagccca catcatatcg ttgatccaat tttttcccaa
     2761 gcgctacaaa ggcaaggtga ccttgttccg gagcagggga catccgctgt tgtgttctta
     2821 cgacggtcaa tgcggctggg acgaacttgc cagccaggtg gaaatgcatg tggttcccgg
     2881 tgaacacgaa agcattttgg aagagccgca tgtgcgagtg ctcgccgcaa aattgaagga
     2941 atgtctgcga cggcttcaat cgccggaaac agtcccggca tccgtggcgg gactgaaaac
     3001 aaaggctgcg gattctcaaa cgggcgcaag cgccaaccag ccaagcgccg attatccacg
     3061 cgagctttgc gtgcatcagc tttttgaaga acaagtcaaa cgcacgcccg atgcgcccgc
     3121 gttgctcgcc ggaggcgaac gcttgaccta cgccgaaatc aatcgccgcg ccaaccaact
     3181 tgcgcatcat ctcgaagcgc tgggtgtcgg cccggatgtg ccggtgggca tcagcctcga
     3241 acgctcgccg gaactcgtga taggtttatt ggcaatcttg aaggctggtg gggcttatgt
     3301 gcccttggat cccgcgtatc caagggagcg attgtcaaaa atgctcgaaa actcgcgcgc
     3361 cccgatcttg ctgacacagc accaattcgt ctcgacattg cctgcggaaa atttgcaggt
     3421 cattctgctc gatgaaccgc ttcctgccgg gttgaaagaa agtaatcctg tcccgcgcgc
     3481 gtcggccaac aatctggcat acgtgatcta cacttcaggt tcgacgggcc agcccaaggg
     3541 cgtcgcgatg gagcatcgtc cgcttgtcaa tctgctctgg tggcagctca aaaattccgc
     3601 gatgggcaag ggcgaccgga cgcttcaatt cgcctccgtg agtttcgatg tttcgtttca
     3661 ggaaattttt tcaacgtggt gcagcggcgg agaattggtg ctcatcagcg aagaggtgcg
     3721 gcacgatccg cagcaactgg cgcgcctgct tgcagaagaa aaggtgaagc ggctcttcct
     3781 tccgttcatc gcattgaacc atctctcgga atcgattggc gctgaagaat cgcgttcgtt
     3841 cagtttgcgc gaagtcatca cggcgggtga acagcttcgc atcacgaaca aaatcaccgc
     3901 attttttgaa gcgttgcccg aatgcacgct gcataatcat tatggcccgt cggaaagcca
     3961 tgttgtcacc gcgtacacga tgagcggccc atccgcaaaa tgggccgcgt tgccgcccat
     4021 tggccaaccg attgccaata cacaaattca tctgctggat gcgaatctga aaccggtgct
     4081 ccgcgatgaa ccgggagaac tttacattgg cggcgattgc cttgcccgcg gttacttgca
     4141 tcgtcccgac ctcacggccg agcggttcgt gaacgatccc ttcagcgcag aaccgggcgc
     4201 aaggatgtac aagaccggcg atcttgcccg ccggttgcct gatggcaacg tggagttttt
     4261 aggccgtgtg gatcatcagg tcaagattcg cggtttccgc gttgagttga gcgaaatcga
     4321 agccgttctt ggcaagcatc cgggtgtgcg cgaatgcgcc gtcgcggccc gcgaagatgt
     4381 tcccagccaa agacgccttg ttgcttacat tgtgcgccag cctgcccacg tcctgacaat
     4441 cgacgaactg cgcgccgccg tgctcgacaa attgccggat tacatggccc cttccgcatt
     4501 cgtctttttg gaagcattgc cattgacgcc cagcggcaag gtcaatcgcc ttgcattgcc
     4561 ggccccggac caaaaccgtc cgcaattggg cggtgaattt gtcgctgcgg ccaacgagac
     4621 cgaggcgcga ttggctggaa tctggagcga ggtgttgaat gtcaaggaag tgggaacgca
     4681 ggataatttt ttcgatctcg gcggcaattc attgtctgcg gcacaggtga tttcgcgcgt
     4741 gcgcgaagcg ttcaaagtgg agttgccggt ctcgtgcttg tttgaagcgc caacgattgc
     4801 ttcattggcg gaaggaatct cgacgcgccg ctggtcggaa ggacgtccag cggtgccgcc
     4861 gctaaaacgc gctccgcgct caggcaatcc gccgctttcc ttcacgcaag gacgtttgtg
     4921 gttcattgac cgcctggagc cgggcagcca tgcctacaac gttcccatgg cgattcggtt
     4981 gcaggggggg cttgatttga aggtgttgca aaacagcctg gacaaaattg tctcgcgtca
     5041 cgaatctttg cgcacctctg tttcatttgc caacgggaac ctggcacagg tgatttcacc
     5101 cgatgcccat ctcaagattt ccgtcgtgaa ttgcgaaaca ttcccgccgt cagagcgggc
     5161 agcccaagca aagaaggcgg tggaaaagca agcgaggcag cctttcaatc ttgaacgcgg
     5221 cccgctggtg cgttgcacgc tcgtgcgctt gagcgagcag gaacacattc tgctggtggt
     5281 gatgcatcat atcatttccg acggatggtc attggcggtg tttttcaagg aacttaatct
     5341 tctctatgat gcgtacgcct ccggaaggct cgcgccaatc cttccggttt tgcccgtcca
     5401 atacgccgat tataccttgt ggcagcagca atggatgcag ggaactgttt tagaccagca
     5461 tgtttcccat tggacggaaa aactcaaagg cgcgccgttg tccttgcaat tgcccatgga
     5521 cccgccgtcg cagaccgcgc cagcgggcaa ttccggccag gaaactttgc ttcttcccaa
     5581 ggcttccagc gaagcattgc agggattgtg tcgccgtgaa ggattgacgc cgttcatggt
     5641 gttgctggca ggactcgcaa tcactttgca aaggtggacg gagcaatcgg acatggtcat
     5701 cggcacggtg gtcgcagggc gcaatcatcg tgagctggag aatttgattg gttgcttcat
     5761 gaatttcgtg ccgttgcgga tcaccgcgac agaaaccgca accggtgcgg aatttctggc
     5821 gcgcgtcaag tccgcggtca tcgatgccaa tgcgcatcaa gaatgtccat ttgaaaaaat
     5881 cgtggaagcc gtcaatccgg agcgcaagac atcgcaaaat cctttgtaca acgtggcttt
     5941 cctgatgcag aattatccga cagccacggt tggcggcagg ttgttggaat cggaaccggt
     6001 gccggtggat ttgcaggctg cgttgctgga tttgaggttt atcgccgagg aatccgcggc
     6061 gggcatttcc gttgcgtgcg agtacaaggc agatttgttc acggcgggga cgattgagca
     6121 attgatggcc tcattcaccg gcgtccttgc cgcattagtc gaagagccgg gcaaacaaat
     6181 caatcaattc cagcttgtcc cgcaacttgc ggcgcaatgc aaggcggcga gggaacgcga
     6241 agaccggcag acgattgccg tggcggcgac gttcaccgcc gaacccgtcg aggacagcct
     6301 gaaatattgg atgaaggaac tcgacatggt cgggcgcgtc gagtttgccc cgtacaatca
     6361 agtgttccag cagttgctcg atccttcgag tctcctgggc acaaatcagc gcgggttgaa
     6421 tgtcatcttc atccgctttg aagattggga aaagaccgag ggcggtccgg agacgcccgc
     6481 agttggagac ccatcggcct tggaacgaag catcggcgaa ttcattactt ctctcaagtc
     6541 gcttataggt cggggcgccg caccctgttt gatttgcctt tgtccaagtt caaaatcagc
     6601 gcttctggat tccgaccgcg cagccctcca tcggcggatg gaagaattgt tgtccatatc
     6661 gttggaaccc atcgccggcg cgtatgtcgt ttcgacgaag gaaatcaacc agctttatcc
     6721 ggtcgcggaa tgtcacgatg cgcgcgccga ggcgttcggg gccattccgt acacagcaac
     6781 atttttcacg gcattgggaa ctgtggtggc gcgaaaattc cacgctttga aacggcccgc
     6841 ttacaaggtc atcgtgctgg attgcgacca gacactttgg tcgggcgttt gcggcgaaga
     6901 tggcccaacc ggcatcgaat tgaatccatc gcgccgcgtg ttgcaggaat ttatgcgcgc
     6961 gcagagcgct tccgggatgc tcctgtgcat ttgcagcaag aacaaccagg cggacgtggc
     7021 tgccgtcttc gagagccgcc gcgagatgcc gttgaaacgc gaacatttcg ccgcgtggcg
     7081 cgtcaattgg caacccaagt ccgaaaactt gaaatcgctc gcgtcggaac tgcgcgtcgg
     7141 gctcgacagc ttcatcttcg tggatgacaa cccggtggaa tgtgccgagg tcgccgcgaa
     7201 ttgtcccgag gtgttgacgt tgcagttgcc agaaccgcct gaagagattc cgcagttttt
     7261 gaatcattgc tgggcgtttg accatctgaa attatcggca gaagaccgtc aacgcacggc
     7321 ttcgtatcag caggagaagg agcgggaaca attgcgctcg caatcgctga acctgtcgga
     7381 tttcctcgca ggactggatt tgaaggtcga gatcgcccgg ctttcctcgg agcaattgac
     7441 gcgc
//
LOCUS       DASZMM010000085         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4971502, whole genome shotgun sequence.
ACCESSION   DASZMM010000085 DASZMM010000000
VERSION     DASZMM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4971502"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VG754_02200"
     CDS             complement(<1..270)
                     /locus_tag="VG754_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWF18045.1"
                     /translation="MSQPFKITSKIRLRDFDPSFHDGLDKDVTREETTRLCLKLGELQ
                     HLLYANCSHSVIMLLQGMDTSGKDSVSRRVLEFVTPAGVETANFKT"
     gene            376..1155
                     /locus_tag="VG754_02205"
     CDS             376..1155
                     /locus_tag="VG754_02205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18046.1"
                     /translation="MKLNDVQKAAQEQFAKQSHRYGRGHILENVEDVRDAVEKLRLPA
                     KAQVLDVATGGGHTGLFIASLGHEVTLADLAQPMLERAAQTAAERGLKVETRQHSAEQ
                     LPYPDGQFDLVTCRVAAHHFSSPADFIRETARVLKPGGFFLLIDGSVADDESEAEEWL
                     HNVEKLRDPSHHRFLTPRGWSELCERNGLKVRSAELHPFKQPDLNWYFETAATSPENR
                     KQVLELVAKAPESARKLFKLGEEDGKIVWWWQRLTLVAEKN"
     gene            1536..>2482
                     /locus_tag="VG754_02210"
     CDS             1536..>2482
                     /locus_tag="VG754_02210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HWF18047.1"
                     /translation="METSLPTVLLRPGEADRIVAGHPWIYSGSVLRVTQPPADGALVQ
                     VKDHRQRFLGVGFYNSKSKISVRMLAPERVTVDEAFFEERIKAALAVRQKHLPGASSF
                     RVVNAESDFLSGLIVDKYEDVLVMQTSALGMDQRKELIADVLQKLFSPRAIVERNDTA
                     FRKFEGLPDANGILKGALSGPVNIKLNGLTFEVDILGGHKTGLYLDQQVNYQRVAELA
                     KGAQVLDCFSFLGGFGLHAARAGAAQVHSLDQSADAIAAATRNATVNGLAGKCSFETV
                     NVFDWLKAHTAVKPHERVIPRFDVIVLDPPSFTRSRATVP"
BASE COUNT          558 a          646 c          756 g          522 t
ORIGIN      
        1 cgttttgaag ttggcggttt caacgcccgc gggagtgaca aattccagca cgcgcctgct
       61 tacgctgtct ttgccgctcg tgtccatgcc ttgcaggagc atgatgacgg aatgcgagca
      121 attggcatag agcaggtgtt gcagttcgcc aagcttcaag cacaaacgcg tggtttcctc
      181 gcgcgtcacg tccttgtcga ggccgtcgtg gaagctggga tcaaaatcgc gcaaccgaat
      241 cttggaagtg attttaaagg gttgtgacat gataagatac ttctaacaca acaagccggt
      301 gataaccacc gcattcaacg gagcgcgaaa tggcgcatat tgcgggcgac cgctggtagg
      361 gcttagaata accccatgaa attgaatgac gtgcagaaag cggcgcagga acaattcgca
      421 aaacaaagcc atcgttacgg acgtggtcat attttggaga acgtcgagga tgttcgtgat
      481 gcggtggaaa aacttcgcct gcctgcgaag gcgcaagtgc tggatgtcgc gaccggcggc
      541 gggcatacgg gcttgttcat cgccagcctt ggacatgaag tcacgctggc ggacctcgcg
      601 caaccgatgc ttgaacgcgc cgcccaaacc gccgccgaac gcggtctgaa ggtcgagacg
      661 cgccagcact cggccgagca attgccatat ccggacgggc aattcgatct ggtgacatgc
      721 cgcgtggcgg cgcatcactt cagttcgccc gccgatttca tccgtgaaac cgcgcgtgtg
      781 ttgaaacccg gcggattttt tctcctgatt gacggttcgg tcgccgatga tgaatcggaa
      841 gccgaggagt ggttgcataa tgtggaaaaa ttgcgcgacc cgagccatca tcgtttcctt
      901 acaccgcgcg gctggagcga actttgcgag cgcaatggtt tgaaagtgcg cagcgcggag
      961 cttcatccgt tcaagcaacc ggacctgaac tggtatttcg aaacggctgc gacctcgccg
     1021 gaaaacagaa aacaagtttt ggaactggtc gccaaagcgc cggaatccgc gcgcaagctt
     1081 ttcaagctgg gcgaggaaga tgggaaaatt gtctggtggt ggcagcggtt gacgctcgtc
     1141 gcggagaaaa attgagccat ttgagggcag cgcgcgccgc aaaaaattta aaaaatttat
     1201 caggaaataa gcggaattac ccccgccaag cggaattaag cggaaaattc cctgaacgta
     1261 tgcggaattt tgccggaaat gttcaggatt tttcctcttg gccgtgtgcg cgacggggga
     1321 agcatccgcg gccaaagtga ttccgttacc ggctggaaaa agccggacgc caaacaccgg
     1381 aggtgctttg tgtgctcctt gtctcacacc gcgaagagag tgacatgccg cctgatgttt
     1441 gtcgaggatt ttcggattgg gtttggcttg gaatcagatg tcaggttaga ttgagtgaaa
     1501 tcgccgtgcg aaacggcgca actgtttaca aaaatatgga aacttcattg cccactgttt
     1561 tgcttcgacc gggagaagcc gatcgcatcg tcgcgggaca tccctggatt tacagcggtt
     1621 ctgtcttgcg cgtcacacag ccgccggccg acggcgcgct ggtgcaggtc aaggaccacc
     1681 ggcaacggtt tctcggcgtc ggattctaca attccaaatc aaaaatcagc gtgcggatgc
     1741 tcgcgccgga gcgcgtgacg gtggatgaag cctttttcga ggagcgcatc aaagcggcct
     1801 tggccgtgcg acaaaaacat cttcccggcg cttcttcctt tcgcgtggtc aacgcggaga
     1861 gcgatttttt gagcgggttg attgtcgata aatacgagga cgtgctggtg atgcagacct
     1921 cggcgttggg gatggaccag cgcaaggagt tgattgcgga cgtgctgcaa aagctctttt
     1981 cgccccgcgc catcgtggag cgaaacgaca cggcattccg aaaattcgag ggattgccgg
     2041 acgcgaacgg gatcttgaaa ggcgcgctca gcggcccggt gaacatcaag ctcaacggat
     2101 tgaccttcga ggtggacatc cttggcgggc acaagacagg tttgtatctc gaccagcagg
     2161 tgaattatca gcgtgtggcg gaactggcca aaggcgcgca ggtgctggat tgtttcagtt
     2221 ttcttggcgg gttcggcttg cacgccgcgc gcgccggggc cgcacaagtc catagcctgg
     2281 accaaagcgc cgatgccatt gccgccgcga cgcgcaatgc gacagtaaac gggcttgccg
     2341 gaaaatgctc gttcgagacg gtgaatgttt tcgactggct caaggcgcat acggcggtca
     2401 agccgcacga acgcgtcatt ccgcgcttcg atgtgattgt gctcgatccg ccgtcgttta
     2461 cgcgcagccg cgcgaccgtg cc
//
LOCUS       DASZMM010000086         4265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4159001, whole genome shotgun sequence.
ACCESSION   DASZMM010000086 DASZMM010000000
VERSION     DASZMM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4159001"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VG754_02215"
     CDS             <1..348
                     /locus_tag="VG754_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18048.1"
                     /translation="VWLFDALYARREQFMGWFTQFPDRRFIDIYTLHGGTKEETETLM
                     GWAKDQHPPLAFISKNEPDITDSDLRQNHFIFMYSDLPHDQVVYKRNQFCEYLKTSGL
                     APIKHLTSPKMTE"
     gene            complement(454..4020)
                     /locus_tag="VG754_02220"
     CDS             complement(454..4020)
                     /locus_tag="VG754_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HWF18049.1"
                     /translation="MKCSSGRMGRALMAALCIFGTCSLPSFAQPRNVSGNPFSDIVRK
                     TEPLTPEQELKAFHLPPGFDIELVASEPEIGKPINMQFDSQGRLWITQSREYPFPAPA
                     DKPARDAIKILSNFDEHGHARSINTFASGLNIPIGIYPYKDGAIGYSIPYIWRFYDTN
                     HTGHAESKEKFLGRFGFEKDTHGMTSSFRRGYDGWLYANHGFHNDSVLTASDGSSIKL
                     NSGNTYRVKIDGSHIEQFTWGRVNPFGLIFDPLGNVFSADCETLPIYQLLRGGYYPSF
                     GKPDDGLGFAPPMMDHKHGSTAIAGIVYYAATNFPSAFRSNIFVGNVMTCRIDRDSLE
                     EHGSTWLAQEKPDFLVSDDPWFRPVDMQQGPDGAIYVADFYNRIIGHYEVPLDHPGRD
                     RERGRIWRIVYRGTHSPAKIEPPQHFNLAHDSTRKLIEQLGNENLTIRMLAMNELTDR
                     IGPSCTNAVAKMMSGRKANLWQKIHGMWVLYRFNALDPKIIEAAAHDKDRALRAHAMR
                     VLSETTPLTESQRALVLTALSDPDGLVQRCAADALGRHPQLENIRPLLDAREWAPSED
                     THLVHVIRMALRDQLLAAANFARLPLTQWSKLDDENVTDVALGVPTTESAKFLLQHLR
                     NYPENREISANDLRHIARYLPEAETGDLVDYVRRQFNDDLDFQLTLFRSIEQGTAQRG
                     DKADEHLREWGADIAKRLANSLEKDEQAWHNTPLEGSAATANPWALQMRASADDDKAS
                     PFLSSLPGGEQLTGVLRSTTFEAPAKLTFWLAGHNGFPDVADQKKNSVRLRAADTGEI
                     LAESFPPRHDVAHRYEWNLSAPGERHQKVYIEVTDGDNAGAYAWLAFGRLEPKVVPWP
                     ITDPRSTGQRQTDVADLVREYKVADIEPQLVNWLKDANTEAVAREAAARALMAIDPEP
                     NIPIISAVGADSAEPPALREKMAGALASANSPAARAALVESLRSAPDKLQVKMALALA
                     GSEEGADALLQLAENGKVSPRLLLDRAIKERLAAAKPAHSEERVAKLTKGLSPASADL
                     QKVLDARLAQFDLAKSDAVKGAKVFSQNCMVCHSVDNQGGAVGPHLDGVGLRGAERLI
                     EDILDPSRNVDPAFRYNIVTLKNGDVIAGLQRREEGAVIVFVDATGKEVPVAKTDIEK
                     NVQSSLSLMPDNFSEAIPVPDFNNLLAFLLSKGSAPTTTAKK"
BASE COUNT          868 a         1230 c         1225 g          942 t
ORIGIN      
        1 gtctggttgt ttgacgctct ttacgcgcgg cgcgaacagt tcatgggctg gttcacgcaa
       61 tttcccgacc ggcgtttcat agacatctac acgctccacg gcgggacaaa ggaagaaacg
      121 gaaacgttga tggggtgggc gaaagaccag catccgccgc tggcttttat ttccaaaaac
      181 gagccggaca tcactgacag tgacctgcgc caaaatcatt tcattttcat gtacagcgac
      241 cttccacacg accaggtggt gtacaagcgc aaccaatttt gcgagtatct caagaccagc
      301 gggctcgccc ccatcaagca tctcaccagc cccaagatga ccgaatgaaa agggatttgt
      361 ggataacgct gaacgcccaa catcgaacat tgaacatcga attatcagcg ttcaaagttc
      421 gatgttggat gttcgatgtt ccctattccc acgtcacttc ttcgccgtcg tcgtgggtgc
      481 gctgcctttg gaaagaagga acgccagcag gttgttgaag tctggcacgg ggatggcctc
      541 ggaaaaattg tccggcatca aagacagcga cgactgcacg ttcttctcaa tgtccgtttt
      601 cgccaccggg acttccttgc cggtcgcgtc cacgaacacg atcaccgcgc cttcttcgcg
      661 ccgctgcaag cccgcgatca cgtcgccatt cttgagcgtg acgatattgt agcggaaggc
      721 cgggtccacg ttgcggctgg ggtcgaggat gtcctcaatc aaccgttccg ccccacgcag
      781 gccgacgccg tcgaggtgcg ggccaaccgc gccgccctga ttatccacgg agtggcagac
      841 catgcaattt tgcgagaaca cctttgcgcc ttttaccgca tcgctcttgg caaggtcaaa
      901 ttgcgcgagc cgggcatcaa gaactttctg caagtccgcg ctggcgggcg aaagtccctt
      961 cgtcaatttg gcgacgcgct cctcagaatg cgccggtttg gcggcggcaa ggcgctcctt
     1021 gatggcgcgg tcgagcaaca ggcgcggcga aacttttccg ttctcggcca gttgcaacag
     1081 cgcgtccgca ccttcctcgc tgccagccaa tgcgagggcc atcttgactt gcaacttgtc
     1141 cggcgcgctc cgcaacgact ccaccaacgc cgcacgcgct gccggcgagt tggccgacgc
     1201 caaagctcca gccatttttt cgcgcaaagc tggaggttcg gcagaatcgg caccgactgc
     1261 gctgatgatt ggaatgttcg gttcgggatc gatggccatt aatgcgcgcg ctgccgcttc
     1321 gcgggccacg gcctcggtat tggcgtcctt gagccaattc accaactgcg gttcgatatc
     1381 ggcaactttg tattcgcgca caaggtcggc gacatctgtt tgcctctggc ccgtcgagcg
     1441 cggatcggtg atgggccacg gcacgacctt cggttccaat ctgccgaacg cgagccacgc
     1501 gtaagcgccc gcgttgtcgc cgtcagtgac ttcgatgtaa actttttggt gacgttcgcc
     1561 tggagcgctc aaattccatt catatctgtg agcgacgtcg tgtcgcggtg gaaaactttc
     1621 cgccaaaatt tctccggtgt cagcggcgcg cagacgaacg gaattcttct tttggtctgc
     1681 cacgtcggga aaaccattgt gacccgccag ccaaaaggtc aatttggccg gggcttcgaa
     1741 tgttgtcgag cgcaggactc cggtaagttg ctcgccaccg ggcaggctgc tgaggaatgg
     1801 cgaggctttg tcgtcatccg ccgaggcgcg catctgcaat gcccagggat ttgcggtggc
     1861 agcgcttcct tccagcggcg tgttatgcca cgcctgctcg tctttttcga gcgagttggc
     1921 aaggcgcttc gcgatgtccg cgccccactc gcgcagatgc tcgtccgcct tgtcgccgcg
     1981 ctgcgcggtg ccctgctcaa tcgagcggaa caaggtcagt tgaaaatcca agtcgtcgtt
     2041 gaattgcctg cggacgtaat caacgaggtc gccagtttcc gcctcgggca gataacgcgc
     2101 gatgtgccgc aaatcgttgg ccgaaatttc acggttctca ggataattgc gcagatgctg
     2161 caacagaaac tttgccgatt ccgtggtcgg cactcccaac gcgacatcgg taacattctc
     2221 gtcgtccagc ttgctccatt gcgtcaacgg caagcgcgcg aaattggccg ccgcaagcaa
     2281 ctggtcacgc aatgccatgc ggatgacgtg caccaggtgt gtgtcttccg acggcgccca
     2341 ttcacgcgcg tcgagcagcg gccggatgtt ttccaattgc ggatgacgtc cgagcgcatc
     2401 ggcagcgcaa cgttgcacaa gcccgtcagg atcggacaac gcggtgagca caagcgcgcg
     2461 ctggctttca gtcaacggag tcgtttccga caacacgcgc atcgcatgag cacggagggc
     2521 gcggtccttg tcgtgcgcgg cggcttcaat gattttcgga tcgagcgcgt tgaagcgata
     2581 caacacccac atgccatgaa ttttttgcca gaggttcgcc ttgcggccgc tcatcatctt
     2641 ggcgaccgca ttcgtgcagg aggggccgat gcggtcggtc aattcattca tcgcgagcat
     2701 gcggatggtg aggttctcgt tgccaagctg ttcgatcaac ttgcgggtcg agtcatgcgc
     2761 gagattaaaa tgctgcggag gctcaatttt tgcaggcgag tgtgtgccgc gatagacgat
     2821 gcgccagatg cgcccgcgct cgcggtcgcg cccgggatgg tccagcggca cttcgtaatg
     2881 accgatgatg cggttgtaaa aatcggccac gtaaatcgcg ccgtccgggc cttgttgcat
     2941 atccaccggg cgaaaccacg gatcatcgct cacgagaaaa tcgggttttt cctgcgcgag
     3001 ccaggtcgag ccgtgttctt ccaacgaatc gcggtcgatg cggcaggtca tcacattgcc
     3061 gacaaaaata ttgctgcgaa atgcgctcgg aaaattcgtc gcggcgtaat agacgattcc
     3121 cgcgatggcc gtggaaccgt gcttgtgatc catcatcggc ggtgcgaagc cgagaccgtc
     3181 gtccggcttg ccgaaactcg ggtaataacc gccgcgcaaa agctgataaa tcgggagcgt
     3241 ctcacaatcg gcggaaaaaa cattgccgag cggatcgaaa atcaggccga atgggttgac
     3301 gcggccccat gtgaattgct cgatgtgcga gccgtcaatc ttcacgcgat aagtgttgcc
     3361 ggaattcagc ttgatgctgc tgccgtcgct cgcggtgagc accgagtcgt tgtggaaccc
     3421 gtggttcgcg taaagccagc cgtcgtaacc gcgccggaac gaactggtca tcccgtgcgt
     3481 gtccttttca aaaccaaaac ggccgagaaa cttttccttg gattccgcgt ggccggtgtg
     3541 gttcgtgtcg taaaaccgcc agatgtaagg aatgctgtat ccgatcgcgc cgtccttgta
     3601 tggatagatt ccaatgggaa tgttcaaacc gctggcgaat gtgttgatgc tgcgcgcgtg
     3661 accgtgttca tcgaagttcg aaagaatttt gatggcatcg cgggccggtt tgtcggcggg
     3721 cgcggggaaa ggatattcgc gcgattgcgt gatccagagc cggccctgcg agtcgaactg
     3781 catgttgatt ggcttgccga tttccggctc ggaggcgacc aactcgatgt caaaaccggg
     3841 cggcagatga aatgccttga gttcctgttc gggcgtgagc ggttccgttt tgcggacgat
     3901 gtccgagaac ggattgcccg aaacatttcg cggctgcgcg aaggacggca gcgaacaagt
     3961 accaaagatg cacagcgccg ccatcaacgc gcgccccatt ctgcctgatg aacatttcat
     4021 gcggattccg acattctatg tgacaatcct cagacgcatc caagcaaaac ttcttcaaaa
     4081 caactattca tggactgatt ggctggaatt gagttcgcca aacacaaccg tttccatttt
     4141 aattttgaat ttttaattct cctctgaccc attccagcgc ttcgttcttc gtcttcaatt
     4201 cgtcctgcaa ttgtttttcg cgcacctcgg cgagcagttc gcccatgcgc ggaccgggct
     4261 tgacg
//
LOCUS       DASZMM010000087         8659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_583002, whole genome shotgun sequence.
ACCESSION   DASZMM010000087 DASZMM010000000
VERSION     DASZMM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583002"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VG754_02225"
     CDS             <1..1773
                     /locus_tag="VG754_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HWF18050.1"
                     /translation="AFDLTGNTNFLNMAKTIFTDLTGGWDTTNVSCPGGIWWNKTHTY
                     KNAIPNELFLLAAIRLHQRTPGDTGSGSYFYWATNEWVWFDGSGMINSQNLVNDGLNG
                     CVNNGQTTWTYNQGVIIGGLTDLYKSTGNTSYLTEAENIANAALTHLVDSKGVLVEPC
                     EPNCGGDGPQFKGIFVRYLAYLYDEARLQTYYNFLFTNAHAVWFNDRNVYNQLGLRWD
                     GPWDSADAARQSCAMMPVSALAEPITANLPFAKGVGDPAFSHSTGSATGTMGWVCSSG
                     ANPTYMLNGPHVMYLPVGPHAVHFRAGVNRLTSSPTNLAQFSVVEENGGTTLATAAAP
                     WNAFKQATVAQDFVLIFTNNSPANPLDFQVTWNNVAGAPSLTLTDVTIDGLANWTAAN
                     LTHNLGRLDGLNGWEADPVRDTGPGYLCLGPGVGGISPGDYSVGFELKVDNFNLDQSQ
                     VATISIVNTDTFVMLASANLTRNQFSNIMYQTFYLNFNAVAGAHYDFRTYWNYSANAP
                     RLTERSVMLRPGATPFFTSVSPAVTMNFIGPPGKTMTVQRTTDLITGSWTNVGTVTVP
                     AYLGSAQFTDSPTNVMSFYRLIIQ"
     gene            1887..2465
                     /locus_tag="VG754_02230"
     CDS             1887..2465
                     /locus_tag="VG754_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18051.1"
                     /translation="MLKPKIIITAVIVWLIMAIQSQAQIQSTNLVPLPATILENLEST
                     TGQVIIKATAPVGSVNCNGAVITIVCKEDTMASTGHKEHGVTVGISINGQPDDRTVID
                     FDELDSLISSLDYLNGITWSVTSLSSFDAIFTTKAGLRVAVFSSKRSGQIEFALRSSR
                     MDTGIIMPPDQRAQFRLLLVGAKAKLVALRNG"
     gene            complement(2491..2566)
                     /locus_tag="VG754_02235"
     tRNA            complement(2491..2566)
                     /locus_tag="VG754_02235"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2531..2533),aa:Lys,seq:ttt)
     gene            2728..3990
                     /gene="larA"
                     /locus_tag="VG754_02240"
     CDS             2728..3990
                     /gene="larA"
                     /locus_tag="VG754_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HWF18052.1"
                     /translation="MNVNLAYGQGHLPVELPDKRTTVIEPSHTPGLRDERAAVIAALE
                     KPIGADGLREWIRPTDTICIGFTDITRATPNDRLIPWLLDFLSFHPREKITLLNSLGT
                     HRPNTRAELEKLLTPEVVRDYRVLNHEPENPAALVQFGTTRDGTPALINRHLAEADVR
                     IITGFIEPHFFAGFSGGPKGIMPGLAGLETVMSNHGEKMIGDPKSTFGITEGNPIWEE
                     MRDIALRVGKSFLLNVTLNEQRQITGVFAGDLLAAHKAGFEFVRHSAMQRVKSPFEIV
                     VTTNSGYPLDQNLYQGVKGMSAGARIIQEGGTLILACECREGVPAKSPLDQLLRSASS
                     PEEILTLLATPGFVRPEQWQAQIQALIQRKARVLIYSALPDEIVRAAHLTPCRDISAA
                     VNECLAQLGPEARVAVLPQGPLTIPYLA"
     gene            complement(3993..5666)
                     /locus_tag="VG754_02245"
     CDS             complement(3993..5666)
                     /locus_tag="VG754_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWF18053.1"
                     /translation="MATCIFGLAAGGAAVAFQKGINLLFNATYGRLAHEPTPVFLAGS
                     FCVIMGTALIVGFLLNSFCREASGSGIPQLKIAFWKDFGYVPWRVVWVKFVAGMVSIG
                     GGSSLGREGPSVHFAGGLASDIAGLMGEAKQNRRNAAAAGAAAGLAAAFNTPIAAVTF
                     VLEEIIEDLNSGFLGSILLASVIGALVVHGLIGKQPAFTLANNVEPSSWWVYGLTPLV
                     AVVSSVVGLAFQKSTLELRAQRKKFERVPGWIRPAMGGFITWVLGATVFIYTGSLGVF
                     GLGYDDLSKALSNGFDWKLAGVLVGVKLVATVACYGFGGCGGIFSPMLFLGGMCGVCI
                     AGGLGHFLHLSAADQIVLAVVGMSTCLGAVVRAPVTSILIVFEMTHQFSLVPVLMLGT
                     LVSQAIRHRFARHNFYEALLIQDGHVLEHVIPPRDLQSWQQLPVSAIANFHPIIATEL
                     DGKLFESLLQNHPYRYFPVTQAGNPPLIVSRKEAEMALAEKRPVRAEPAVTCVPSQTI
                     RELQMLIVESNQQVVLVLDGEKGKVLGLVTLHDLLRAQVSLGRESAGAF"
     gene            5852..7318
                     /locus_tag="VG754_02250"
     CDS             5852..7318
                     /locus_tag="VG754_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWF18054.1"
                     /translation="MNNWHLGKIGRRLWAAFLAAMLVGIILAPSLRAQDATNHPSGRR
                     EPNIIFILADDLGYGDLGCYGQNEIKTPNIDKLAEEGMRFTSFYAGSTVCAPSRCALM
                     TGKHNGHSRIRGNGEIPLSPEDVTVAEVLKKAGYQTAAIGKWGLGERNSTGVPGKKGF
                     DQWFGYLNQVDAHNYYPLFLNRSDPRHGDRQVQLPENEGGQKGRYSDDFFTDAALNVI
                     RLDQPKWYTKYQPFFLYLAYTIPHANDELGTATGNGMEVPSDAPYSGESWPQVEKNKA
                     AMITRLDTDVGLILEKIQKLKMDNTVVIFSSDNGTHKEGGVDPKFFKSAGLLRGIKRD
                     LYEGGIRVPFIVWWPGRVKPGTTSDLPAAFWDFLPTAADIADAKAPQDIDGISFLPTL
                     LGQKQTNWHEFLYWEFHEDGFNQAVRMGDWKAVRFGVDGPLQLFNLKDDIGEKKNVAA
                     EHPEIVAKIEDYLKTARTEDSNWPVKTAAESSKKEYGK"
     gene            7337..8173
                     /gene="purU"
                     /locus_tag="VG754_02255"
     CDS             7337..8173
                     /gene="purU"
                     /locus_tag="VG754_02255"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWF18055.1"
                     /translation="MSTQVLLVECDDRRGLVHAITGVLLKHGVNVVGNHEFVDRASAR
                     FFMRTEFDGAVAASQLEAEVREALPPNASVRLSDLKPKRIVVLVSKEHHCLGDILIRH
                     AFDELNAHVLAVISNHDVLGPLAAKFKLPFHHLSHEAFSRPEHEAAMLKIIEPLQPDF
                     LVLAKFMRVLSPDFVDRFPARIVNIHHSFLPAFVGAKPYQQAFERGVKVIGASAHFVT
                     EKLDEGPLIVQQVIPVDHTHTARDLSQAGRDVEQMVLARALRLVLEDRVFLCDNRTVI
                     FD"
     gene            complement(8197..>8659)
                     /locus_tag="VG754_02260"
     CDS             complement(8197..>8659)
                     /locus_tag="VG754_02260"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2950 family protein"
                     /protein_id="HWF18056.1"
                     /translation="YATKLNSTPGQTDGLYWPAGTSGRPSPLSSFVAQACLERYNWSG
                     QGPQPFHGYFFKILTRQGPAAPRGKMNYIHKGEMTAGFAIVAYPVRWGESGIMTFIVN
                     QDGTVYQRSFGDKTASVAERMKEYNPDSSWNVVRDPGIVDLTVTQPVGNVR"
BASE COUNT         1996 a         2462 c         2350 g         1851 t
ORIGIN      
        1 gcgttcgact taactggtaa cacgaatttc ctgaacatgg ccaaaaccat tttcaccgac
       61 ctcaccggcg gttgggacac caccaatgtt tcgtgtcccg gcggcatttg gtggaacaag
      121 acgcacactt acaaaaatgc cattccgaac gaattgttcc tgctggctgc gatccgcctg
      181 catcaacgca cgcccggcga caccggatcg ggcagttact tttattgggc cacgaacgaa
      241 tgggtttggt tcgatggaag cgggatgatc aattcacaga acctcgtcaa cgacggtttg
      301 aacgggtgcg tgaacaatgg ccagaccaca tggacttaca atcaaggcgt gatcatcggc
      361 ggcttgacgg acttgtacaa aagcaccggg aacacgagct atttgacgga agccgaaaac
      421 atcgccaacg ccgccctcac tcatcttgtg gacagcaagg gcgtgttggt cgagccgtgc
      481 gagccgaatt gcggcggcga tggcccgcag ttcaagggaa ttttcgtgcg ctatctcgcg
      541 tatctttacg atgaggcacg gcttcaaacc tattataatt ttctcttcac caacgcccat
      601 gcggtctggt tcaacgaccg caatgtttac aaccaactcg gtttgcgctg ggacggacca
      661 tgggacagcg ccgatgccgc gcgtcaaagt tgcgcgatga tgccggtcag cgcgctcgcc
      721 gagccgatca ctgccaacct gccatttgca aaaggcgtgg gcgacccggc attcagccat
      781 tcgacaggca gcgcgacagg gacgatgggc tgggtttgca gcagcggtgc gaaccccact
      841 tatatgctga acgggccgca tgtgatgtat ttgcccgttg gcccgcacgc ggtgcacttt
      901 cgcgccggcg tcaaccggtt gacttcatca ccaacgaacc ttgcccagtt cagcgttgtg
      961 gaagagaatg gcggcacgac cttggccact gcggccgcgc cttggaatgc cttcaagcaa
     1021 gccactgtcg cccaggattt cgtcctgatc ttcacgaaca attcgcccgc caatcctttg
     1081 gattttcaag tcacctggaa caatgtcgca ggcgcgcctt cgttaacgct caccgatgtc
     1141 acgattgacg ggcttgccaa ttggaccgcg gccaatctca ctcacaacct tggccggctc
     1201 gacggtctca acggctggga agcggatccc gttcgtgaca cgggcccggg ttatttgtgc
     1261 ctcggccccg gagtcggcgg aatttcaccc ggcgattatt cggttggttt cgaattgaag
     1321 gtcgataact tcaatctgga ccaatcgcag gtggcgacca tttcaatcgt gaacacggac
     1381 acgttcgtta tgctcgcgtc tgcgaacctg acgcgaaacc aattttccaa catcatgtat
     1441 caaacgttct acctgaactt caacgcggtc gcgggcgcgc attacgattt ccgcacatac
     1501 tggaattaca gcgcaaacgc tccgcgcctg accgaacgaa gcgtgatgtt gaggccgggt
     1561 gcgacgccgt tcttcacatc tgtttcaccg gcggtcacca tgaacttcat tggtccgccc
     1621 ggcaaaacca tgacagtcca gagaacgacg gatctcatta ctggctcatg gacgaatgtt
     1681 ggcacagtca ccgtgcccgc ttatctggga agcgcgcaat tcaccgattc gccgaccaat
     1741 gtgatgagtt tttaccggct gatcatacag tgagtttcat tcgtagatag cgaaatggcg
     1801 cgtaactttt gatgaaactt tgctgccgtc ttggtgttgc ttacctgata caaagttgcg
     1861 tccgtcaatc tttaatcgga atgattatgt tgaaacccaa aattatcatc acggcggtga
     1921 ttgtctggtt aatcatggca atccaatcac aggcccaaat tcaaagcaca aatctcgttc
     1981 ctctgcccgc gaccattctc gaaaacttgg agagcaccac cgggcaggtg atcatcaagg
     2041 ccactgcgcc ggtcggttcg gtcaattgca atggggccgt catcaccatc gtgtgcaagg
     2101 aagatacgat ggccagcacc ggccacaagg agcatggcgt cacagttgga atttcaatca
     2161 atggacagcc ggacgataga accgtgattg attttgacga actcgattcg ctgatcagct
     2221 cccttgatta cctgaacggc atcacgtggt cagtgacttc attgtccagt tttgatgcca
     2281 tcttcaccac gaaggccgga ttgcgagtgg cggtattcag cagcaaacgc tcgggccaga
     2341 tcgagtttgc attgcgcagc agcaggatgg atacaggcat tatcatgccg cccgatcaac
     2401 gcgctcaatt ccgattacta ttggtcggtg caaaggccaa gcttgtggct ttgcggaatg
     2461 gataaagcaa aaaactttga ggttttgaaa tggtgggttg gcagggactc gaaccctgga
     2521 ccaatgcctt aaaagggcac tgctctacca actgagctac caacccactc tcccgttcgc
     2581 gggattggac cgatgctgaa gattgcggcc aatcaaatgc gccgcgaaag ctaatcaacc
     2641 tgcccgctga gcgcaaggtt ttttcaagcc gatttggcgc tttccccggg ccgcatttta
     2701 cggtatccag taactaacat tgtttgcatg aatgtcaatc ttgcctatgg ccagggccat
     2761 ttgccggtgg aattgccaga caaacgaacc accgtcatcg agccgtcgca cacacccggc
     2821 ctgcgcgatg aacgcgccgc cgtcatcgcc gcattggaaa aacccatcgg cgcggatggc
     2881 cttcgggaat ggatacgccc gacggacacg atttgcatcg gcttcaccga catcacacgc
     2941 gcgacgccca atgaccggct cattccgtgg ttgctggatt ttctttcatt tcatccgcgc
     3001 gagaaaatca cactgctcaa ttcgctcggc acgcatcgcc cgaacacgcg cgccgaactg
     3061 gaaaaacttt tgacgcctga agtcgtccgc gattatcgcg tgctcaatca cgagccggaa
     3121 aatcccgcag cgctcgttca gtttggaact acgcgtgacg gcacgccagc gttgatcaac
     3181 cggcatctcg ctgaggccga cgtgcgcatc atcaccgggt tcatagagcc gcatttcttt
     3241 gccgggttca gcggcgggcc aaagggcatc atgccgggtc tcgctggctt ggagacggtg
     3301 atgagcaatc acggtgagaa aatgatcggc gatcccaaat caacatttgg aattaccgag
     3361 ggcaatccga tttgggagga gatgcgcgac atcgcgttgc gcgtgggaaa aagttttctg
     3421 ctgaacgtca cgctcaacga gcagcggcag atcaccggcg tgttcgcagg cgatttgctg
     3481 gcggcgcaca aggccggttt tgaattcgtg cgccactcag caatgcagcg cgtgaagtcg
     3541 ccgtttgaaa tcgtggtcac gaccaacagc ggctatccgc tcgaccagaa tctttaccaa
     3601 ggcgtcaagg ggatgagcgc gggcgcgcgc atcatccagg aaggcggcac gctgattctc
     3661 gcctgcgaat gccgcgaagg tgtgccggcg aaaagtccgc ttgaccagct tttgcgcagt
     3721 gcgtcgagtc cggaagaaat cttgacgttg ctggcgacgc ccggtttcgt gcgtcctgaa
     3781 caatggcagg cgcagatcca ggcgctcatc cagcgcaagg cgcgcgtgct gatttacagc
     3841 gcattgccgg atgaaatcgt ccgcgcggcg catctcacac cttgtcgtga catcagcgca
     3901 gcggtgaacg aatgtcttgc gcagcttgga cccgaagcaa gggtcgcagt gttgccgcaa
     3961 gggccgttga cgatacctta tctcgcttga agttaaaacg ctcccgcaga ttccctgccc
     4021 aaggaaacct gagcgcgaag caaatcgtgc agcgtgacca atccaagcac ttttcccttt
     4081 tcgccatcca acacaagcac tacttgttgg ttggattcga caatcagcat ttgaagttcg
     4141 cggatggttt gcgaggggac acaggtgact gcgggttcag cgcggacggg acgtttttcg
     4201 gccaacgcca tctcggcttc tttgcgggag acaatgagcg gcgggttgcc ggcttgtgtc
     4261 accggaaaat atcggtaggg atgattttgc agcagggact caaataattt gccgtcaagt
     4321 tcagtcgcga tgatgggatg aaaattggcg atggcggaaa ccggaagctg ttgccagctt
     4381 tgcaaatccc gcggtggaat gacgtgttcc agcacatgac cgtcctggat gagcaacgcc
     4441 tcgtagaaat tatgcctggc aaaacggtgg cgaatggcct ggctcacaag ggtgccgagc
     4501 atcaggacag gcacgagtga aaattggtgc gtcatctcaa atacaatcag gatgctagtg
     4561 accggcgcgc gcaccactgc gccaaggcag gtgctcatgc cgacgaccgc aaggacaatc
     4621 tggtctgccg cgctcaggtg gaggaaatgt cccagcccgc cggcgatgca cacgccgcac
     4681 atgccgccca aaaaaagcat cggcgaaaaa atcccgccgc aaccgccaaa tccgtagcaa
     4741 gcgacggtcg ccacaagttt gacccccacc aacacgccgg ccaatttcca atcgaatccg
     4801 ttgctcaacg cttttgacaa atcatcatag cccaaaccaa acactccaag gctgccggtg
     4861 taaatgaata ccgtcgcacc gagcacccac gtgatgaatc ctcccatcgc cggccttatc
     4921 caaccgggca cgcgttcaaa ttttttgcgt tgggcgcgca attccaatgt ggatttttgg
     4981 aaagccaacc cgacgacgct gctcaccact gcgaccaaag gcgtcaatcc ataaacccac
     5041 catgaggaag gctcgacgtt gttggcaagt gtgaaggcgg gttgcttgcc gatcaaacca
     5101 tgcacgacaa gggcgccgat gaccgatgcg agcaagatgc tgccaagaaa accgctgttc
     5161 aagtcctcga tgatttcttc cagcacgaac gtgaccgccg cgatcggggt gttgaacgcc
     5221 gcggccagac ctgcagccgc gcccgcagcg gcggcgttgc ggcggttttg cttcgcctcg
     5281 cccatcaaac cggcaatgtc cgatgccaat ccgccggcga aatgaacgct cggaccttcg
     5341 cgccccaaac tcgaaccgcc gccgatgctg accatgccgg cgacaaattt cacccaaacc
     5401 acccgccacg gcacgtatcc aaaatctttc caaaacgcga tcttgagttg cgggatgccg
     5461 ctgccggaag cttcgcggca aaaggaattc aggagaaagc cgacgatgag cgcggttccc
     5521 atgatgacgc agaaactgcc ggcgagaaac acgggagttg gttcatgcgc gagtctgccg
     5581 tacgttgcgt tgaaaagcag gttgatgccc ttttggaatg ccactgccgc cccgcccgcg
     5641 gccaaaccga aaatgcaggt ggccatgata gcgcgggtgc gttgcggaag tttgcgtaga
     5701 tattgtctta cagataataa agtcacccgt tcactgttgc tgtttgtttg aaggttttca
     5761 agtgcgggtt gtccaaccag cgacaatcaa ttgcgtctcg ccgaaataat gggttgacgc
     5821 ggcagtggcg aatgcactta cagtctttaa catgaacaat tggcatttgg gaaaaattgg
     5881 gcgtcgtttg tgggcagcat tcttggcggc gatgttggtg ggaatcattt tggcgccatc
     5941 attgcgcgcg caggacgcca caaatcatcc gtccgggcga cgcgagccga atatcatttt
     6001 catccttgcg gacgacctcg gctatggcga tctcggctgt tatgggcaaa acgagatcaa
     6061 gacgcctaac attgacaagc tcgccgagga aggaatgcgt ttcaccagct tttacgccgg
     6121 cagcaccgtt tgcgcgccgt cgcgttgcgc attgatgacc ggcaaacaca atggccattc
     6181 tcgcatacgc ggcaacggcg agattccgtt gtctcccgag gacgtgaccg tggccgaggt
     6241 gttgaagaag gccggttacc aaactgccgc aatcggcaaa tggggtctcg gtgagcgcaa
     6301 ttcaactggc gttcccggca agaaggggtt cgaccaatgg ttcggatatc tcaaccaggt
     6361 ggacgcgcat aattattatc cgctgttcct caaccgcagc gatcctcgcc acggcgaccg
     6421 tcaggtgcaa ttgcccgaga atgagggagg ccagaagggc agatattcgg atgatttttt
     6481 tacggatgcg gcgttgaatg tcattcgact ggaccagccc aagtggtaca caaaatatca
     6541 gcccttcttt ctttacttgg cttacaccat tccgcacgcc aacgacgaac tcggcacagc
     6601 gaccgggaac ggcatggaag tccccagcga cgctccctac tccggtgaat catggccgca
     6661 agtcgaaaaa aacaaggccg ccatgatcac gcggctggac acagacgtcg gtttgattct
     6721 cgaaaaaatt caaaagctca agatggacaa caccgtcgtc attttctcca gcgacaatgg
     6781 gacgcacaag gaaggcggtg ttgatccaaa atttttcaaa agcgccgggc tgttgcgcgg
     6841 catcaaacgc gatttgtacg aaggcggcat tcgtgtgccg ttcatcgttt ggtggcccgg
     6901 acgtgtcaag cccggcacga cgagcgactt gcctgccgcg ttttgggatt tcctgccgac
     6961 cgccgcggac attgcggatg caaaggcgcc gcaagacatt gacggaattt cgtttcttcc
     7021 cacgctgctt ggccagaagc aaaccaattg gcacgaattc ctgtattggg aatttcatga
     7081 agacggtttc aatcaggccg tccgcatggg cgattggaag gcggtccgtt ttggcgtgga
     7141 tggtccactt cagctcttca acttgaaaga tgacatcggc gaaaaaaaga acgtcgccgc
     7201 cgagcatccg gaaatcgtcg cgaagattga agattatctg aaaaccgccc gcaccgagga
     7261 cagcaattgg ccggtgaaga ccgcggcaga aagttccaag aaggaatacg gaaaataggt
     7321 tgcggaagaa attcggatga gcacgcaagt cctcctcgtg gaatgtgacg atcgccgcgg
     7381 gcttgtccac gccatcaccg gcgtgttgct caagcatggc gtcaatgtcg tgggcaacca
     7441 tgagttcgtg gaccgcgctt cggcccgttt cttcatgcgc accgagttcg acggagccgt
     7501 cgccgcaagc caactcgaag ccgaagttcg cgaagcgttg ccgcccaacg caagtgtgcg
     7561 gctttccgat ttgaaaccca aacgcatcgt ggtcctcgtt tccaaggaac accattgtct
     7621 tggtgacatc ctcatccgcc atgcgttcga cgaactcaat gcgcacgttc tcgcagtcat
     7681 cagcaatcac gacgtccttg gaccgctggc cgcaaagttc aaactgccgt ttcatcatct
     7741 cagtcacgaa gcgttttcgc gcccggaaca cgaagccgca atgctgaaaa tcatcgagcc
     7801 gctacaacct gattttctcg tactggcaaa atttatgcgc gtgctgtcgc cggattttgt
     7861 ggaccgtttt ccggcacgca tcgtcaacat ccaccattca tttttgcctg cgtttgttgg
     7921 ggcaaagcca taccaacagg cctttgaacg cggcgtgaaa gtgattggcg cttccgcgca
     7981 tttcgtcacc gagaaactgg acgaaggacc gctcattgtc cagcaggtca ttcccgtgga
     8041 ccacacgcac acggcgcgcg acctctcgca agccggccgc gacgtcgaac agatggtcct
     8101 tgcccgcgcg ttgcgtttgg tcttggaaga ccgcgttttt ctttgtgaca atcgcacggt
     8161 catttttgat tgaagaggat ccagaaactt cacaccttat cgcacattcc caaccggttg
     8221 ggtcactgtc aaatcaacaa tgcccgggtc ccttaccaca ttccacgaac tgtccgggtt
     8281 atactccttc atccgctcgg cgacgctcgc agtcttgtcg ccaaagcttc gctgataaac
     8341 agtcccgtcc tggttcacga taaaggtcat tatccccgat tcgccccaac gcaccggata
     8401 tgcaactatt gcaaaaccgg cggtcatttc tcccttgtgg atgtaattca ttttgccacg
     8461 cggcgcagcg ggaccttgcc gcgtcaggat tttgaaaaaa tatccgtgaa atggttgagg
     8521 accctggccc gaccaattgt atcgctccaa acaagcttgg gccacaaatg aggacaacgg
     8581 gcttggccgc ccgctcgttc ccgcgggcca atacaaacca tccgtctgcc ccggcgtgct
     8641 gttcaatttt gtcgcatag
//
LOCUS       DASZMM010000088         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3598255, whole genome shotgun sequence.
ACCESSION   DASZMM010000088 DASZMM010000000
VERSION     DASZMM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3598255"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VG754_02265"
     CDS             <1..414
                     /locus_tag="VG754_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWF18057.1"
                     /translation="ERDISHSSVERIIFPDSCTLLDYMLVTLAKLTDGLIVYPENMKR
                     NMELTLGMWNSQTVLLELIRKGLTREAAYDLVQRNAMKTWQAKHAGDAQANFKTQLLA
                     DPEVAKHLSAAELDRLCNLDFHFKEIKKRFKSLGL"
     gene            568..912
                     /locus_tag="VG754_02270"
     CDS             568..912
                     /locus_tag="VG754_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18058.1"
                     /translation="MATLFRRYLILLTIAFWMGGFTFYTGVVIPTGNEVMGGERDVGF
                     ITQQVTNWLNTIGVLSLVVLGCNAWFERQGILRLLMLFSWLAMTLLQAGFFMSMPCST
                     AGWTPPRTKFTV"
     gene            complement(1176..>2630)
                     /gene="mnmE"
                     /locus_tag="VG754_02275"
     CDS             complement(1176..>2630)
                     /gene="mnmE"
                     /locus_tag="VG754_02275"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HWF18059.1"
                     /translation="GAAVFGRARRGKRRRKLPRSSVTRNDSGMMEDTIAAIATPLGEG
                     GLAVIRISGPDALALADKCFVALGKSAVPPSAAKTHTLHYGKIMRDGRSVDEVMLAVM
                     RGPRTLTREDVVEITCHGGLLPARLVLETVLKNGARLAEPGEFTRRAFLNGRIDLAQA
                     EAVADLISARTELALAAANEQLAGKLSQRINTLRDDLVKTLAHIEAHIDFPDEDIAPD
                     TRAQLLARLDHGVKFMDELLRTANEGQILRRGIRAAIIGRPNAGKSSLLNQLLGHDRA
                     IVSPIPGTTRDTIEETANIRGLPVVFVDTAGLREAGDEIEAEGVRRSRESLARAEFIL
                     QILDNSEPLTKADENFLAEFAGKKRILVRNKIDLPTRLKLPDGIKSPVIEICCLTGKG
                     MEPLKDAIKELVWAGEIRAEMLQVMINARHQEALSRARAAAKRAGEALQSDQTLELVA
                     MDLRIAMNAVGEIVGKTTTEDLLDSIFSQFCIGK"
BASE COUNT          568 a          787 c          667 g          608 t
ORIGIN      
        1 gaacgcgaca tcagccacag ctccgtcgag cgcatcattt ttcccgattc ctgcaccctg
       61 ctcgattaca tgctcgtcac gctcgcgaag ctgaccgacg gcctcatcgt ttatcccgag
      121 aacatgaagc gcaacatgga gttgacgctc ggcatgtgga attcgcaaac cgttttgctt
      181 gaactcatcc gcaaaggatt gacccgcgaa gccgcctacg accttgtcca acgcaacgcc
      241 atgaaaacct ggcaggccaa acacgccggc gacgcccagg ccaatttcaa aacacaactc
      301 ctcgccgatc ccgaagtcgc caaacacctt tccgccgcgg aacttgaccg tctctgcaac
      361 ctcgatttcc atttcaagga gatcaagaaa cgctttaaga gcttgggatt gtaattctat
      421 aacgtaggag ctgacgtaag gaggctctct ctgataggaa gctttaaaaa gaaatagttt
      481 taaaaccgca gactacctta ttgaaagcct cgaataaaaa cttctcgaca ctcatttttg
      541 cgccttccaa gcttgcatcc gcaaataatg gcaacgctgt ttcgacgtta tctgatcctg
      601 ctgaccatcg ctttctggat gggagggttc acgttttaca ccggcgtggt catcccgacg
      661 ggaaatgaag tcatgggcgg cgagcgtgac gtgggattca tcacccagca ggtcaccaat
      721 tggctgaaca ccatcggcgt cctgtccctt gtcgtcctcg gctgcaacgc gtggtttgaa
      781 cggcagggaa ttctccgctt gctgatgctc ttttcatggc tggcgatgac gctgcttcaa
      841 gcgggctttt ttatgtccat gccgtgctcg acggcaggct ggacaccgcc acgcacaaaa
      901 ttcacggtgt gatggcgagc ttcttcaact ggcatcgcct ttacatactc atcgccagcg
      961 cccaatgggg attcgcgctg atttatctct ggcttgcgat tgttctttgg cgaagggtgg
     1021 acaataaagg agcacaagtg ttcccagcgc ccagcggata attggatgca aatacgttgg
     1081 caggccttgt atccccgacg ggtcttcatg cggatttgga atttgaaatt gagatttcaa
     1141 atcggataag aggctcctca cacagcttaa cgccctcact tcccaatgca aaactggctg
     1201 aaaatggaat cgagcaaatc ttcggtggtc gttttgccga caatttcacc gacggcgttc
     1261 atcgcaatcc gcaaatccat cgcgaccagc tcaagcgttt gatccgattg caacgcttcc
     1321 cctgcccgct tggccgcggc gcgcgcccgt gacaacgcct cctgatgccg cgcattgatc
     1381 atcacttgca acatctccgc gcggatttca cccgcccaaa ccagttcctt gatggcgtct
     1441 ttcagcggct ccattccctt tccggtaagg cagcagattt caatcaccgg cgatttgatt
     1501 ccatcgggca atttcaatcg cgttggcaaa tcaatcttgt tgcgcaccaa aatgcgtttc
     1561 ttgccggcga attctgcgag aaaattttcg tccgccttcg tcaaaggctc ggaattgtcc
     1621 aaaatctgca aaataaattc cgcgcgcgcc agcgattcgc ggctgcggcg cactccctcg
     1681 gcttcaattt catcgcccgc ctctcgcaat cctgcggtgt ccacgaaaac aaccggcagc
     1741 cctcggatgt tcgccgtctc ctcgatcgtg tcgcgcgtcg tgccgggaat tggcgatacg
     1801 atcgcgcgat catgccccaa taattggtta agcagactcg attttccggc gttcggccgg
     1861 ccgataatcg ccgctcgaat gccgcgccgc aaaatttgtc cctcattggc ggtgcgcaac
     1921 aattcgtcca tgaatttcac gccgtggtcc aaacgcgcca aaagctgcgc gcgggtgtcg
     1981 ggcgcgatgt cttcatcagg aaaatcaatg tgcgcctcaa tgtgcgcgag agttttgacg
     2041 aggtcatcgc gcagcgtgtt gatgcgttgc gagagttttc cggccagttg ttcattggcc
     2101 gcggcaaggg cgagttccgt gcgcgccgaa atcaaatccg cgacggcttc ggcttgcgcc
     2161 aaatcaatcc ggccatttaa aaaagcgcgt cgggtgaatt cgcccggctc ggcaagtcgc
     2221 gcgccatttt tcaaaactgt ttccaacacg agtcgcgcgg gaagcaatcc gccgtgacag
     2281 gtgatttcca ccacgtcttc acgggtcaac gtgcgtgggc cgcgcatcac cgcgagcatc
     2341 acttcatcca cactgcgccc gtcgcgcatg attttgccgt aatggagtgt gtgcgttttt
     2401 gccgccgatg gcggaacggc tgacttgcca agcgccacga aacatttgtc ggcgagggcg
     2461 agggcgtccg gccccgagat gcgtatcacc gcaagcccgc cttcgcctag aggcgtggca
     2521 atcgcagcaa tcgtgtcttc catcataccc gaatcattcc gagtcactga tgaccgcggc
     2581 aatttccttc ggcgttttcc gcgtcgcgcc cttccaaaaa cagccgcgcc
//
LOCUS       DASZMM010000089         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4426633, whole genome shotgun sequence.
ACCESSION   DASZMM010000089 DASZMM010000000
VERSION     DASZMM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4426633"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1089
                     /locus_tag="VG754_02280"
     CDS             16..1089
                     /locus_tag="VG754_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18060.1"
                     /translation="MELQNGSQHFEAILKSDPRTWTTYAPGTRLQLTGVYASEDRENH
                     GAATYPFEILLNNAAGIEVLQRPSWWTVRHTIMIAAALGAALGIAFIWITLLHRKVEQ
                     RTTQLQKEIEARQLVEQHRVMEQERARVARDLHDELGTGLTEVSMLGELGTNPAVTLE
                     EKERCFQQITSSARALVTGLDEIVWAINPGYDSVSSAATYYSFFADRFLRLAGITCRL
                     HIPAHLPEWPLASNVRHSVFLACKEALNNIVRHSGASEVEFKVEAPDQELFIGIRDNG
                     KGIQSGGFTPGNDGVLGMQKRLQEFGGSCRITGEPGKGTTVEFRLPLRLFFKADSGAV
                     SADCPQAGNVEADPPRYDKRSDC"
     gene            1067..1693
                     /locus_tag="VG754_02285"
     CDS             1067..1693
                     /locus_tag="VG754_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF18061.1"
                     /translation="MTNVAIVEDSRQTRETLERLINLRPDYHCVCVCETAEEALKVVP
                     RHQPHIVLMDVQLPGMPGIQCASRLRELLPDTRIIMLTVYEDPDRIFSAMRAGASGYL
                     LKRSTPDQLFNAIREVQEGGAPMSAPIAVKVFSYFRTQETDSANTEKLSAREYEILEL
                     IVHGFPNKEIAARLAVSNETVRWHLNHIYRKLQVHSRTEAALKFRPHK"
     gene            1836..2714
                     /locus_tag="VG754_02290"
     CDS             1836..2714
                     /locus_tag="VG754_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18062.1"
                     /translation="MMRLRPAKMPNGFTLIELLVVIAIIAILAAMLLPALNKAKVRAT
                     MATCVSNQKQLTVAWIMYADDNQDNLIGTQTPAFSAGPGYWRYSSYNPLELGSVTGMS
                     AQDIHIRELQLAYKDASLYQYAPNLSVIHCPSDRRYDSPVGANLTTTPSSAPGYFAFG
                     SYAGVGSLNGTLSQITKLTAIKHPTDRFIWVEENDPRGECNGSWDQSYFTAPPGFAGS
                     KLEDSTASWHVRSSTFGWCDGHAESHGWIDGANIAYALNMDPNKYTSSSATPSTGNSP
                     HDLYFLANGWATKANP"
     gene            2739..>3828
                     /locus_tag="VG754_02295"
     CDS             2739..>3828
                     /locus_tag="VG754_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF18063.1"
                     /translation="MKTQKKAFCPGTILLNAVALAFLLTANAWAADYQSTVLADHPIG
                     YWPLNLAVDTGGTAADLSGNTNIGTYDGVFAQVPGPSSFITNAVSFDGATTLVDLSAG
                     SHTDLLNFGGTITMEAWVQPATPGISGDILAKGYDSSQTDDELEMRADSGDYHGGTYN
                     DDAGDLGVSGGTESTNWTHVVCTWDGTNWNLYVNGELTGTGADANGAINFNDAWAIGT
                     GTANGISRLFAGNICQVALYTNALTPAQVLTHYLIGLYGTTTPLPAIITQPVPHTVYT
                     NASATFSVTADPLSGPFGYQWYKGASAISDQTNASLMLTNVQAAAVAGYSVVLTNTYG
                     ASTSSVAALTLVPPATHYDAMVFGDHPLA"
BASE COUNT          904 a         1047 c         1061 g          816 t
ORIGIN      
        1 caagatgaac gcgaattgga actgcaaaac gggtcacaac atttcgaagc catattgaaa
       61 tcggacccgc gcacctggac cacctacgcg ccaggcaccc gcctacaatt gacaggcgtc
      121 tatgccagcg aagaccggga aaaccatgga gcggcaactt atccattcga gatcctgctg
      181 aataacgcgg cgggaattga agtactgcag cgcccatcgt ggtggactgt gcgccacacc
      241 atcatgattg ccgctgcgct cggggctgcg ctgggcattg cgttcatctg gatcacgttg
      301 ctgcatcgaa aagtcgagca gcggacgacg cagttgcaga aggaaatcga agcgcggcaa
      361 ttggtcgaac aacatcgcgt catggagcag gagcgcgccc gagtagcgcg ggatcttcat
      421 gatgaattgg gcaccggatt gacggaggtg agcatgttag gtgagctagg cacgaacccg
      481 gcggtgacgc tggaggaaaa agagcgttgc ttccaacaga tcacctcctc ggctcgtgca
      541 ttggtcacgg gtttggatga aatcgtctgg gcgatcaatc cgggttacga ctcggtgagt
      601 tccgcggcga catactactc attttttgct gatagatttt tgaggctcgc gggcattact
      661 tgccggcttc atattccggc gcatcttccc gaatggccgc ttgcttccaa tgttcggcat
      721 agtgtctttc tggcgtgcaa ggaagccttg aacaacattg tgcggcattc cggggccagc
      781 gaagtcgagt tcaaagtaga ggcgcccgat caggagcttt tcatcggcat tcgggacaac
      841 ggaaaaggca ttcagtcggg cggctttacg cccgggaacg atggggtcct gggaatgcaa
      901 aaacggctgc aggagtttgg cggaagctgc cgcattactg gcgaaccagg aaaaggcacg
      961 accgtcgaat ttcgattgcc cttgcgtcta tttttcaagg cagattcagg agctgtgtcc
     1021 gctgattgcc cgcaggctgg aaacgttgag gctgatccac cacgatatga caaacgtagc
     1081 gattgttgaa gacagccgcc aaacgcggga aacgctggag cggctgatta acctgcgccc
     1141 ggattaccat tgcgtttgcg tgtgcgagac agcggaagaa gctttgaaag tcgtgcctcg
     1201 tcatcagccg catatcgttt tgatggacgt tcaattgccg ggaatgccag gcattcaatg
     1261 cgccagccgt cttcgggaac ttcttcccga cacgcgcatc atcatgttga ccgtatacga
     1321 agatcccgac cggattttca gcgccatgcg cgcgggcgcc agcggatacc ttttgaaacg
     1381 ttcgacacca gaccaattgt tcaacgcgat tcgagaggtg caggaaggcg gagcacccat
     1441 gagcgcgccg atagcggtca aggtcttctc ctattttcga acccaggaaa cggacagtgc
     1501 caacacggaa aagctttccg cgcgcgaata cgaaatcctg gaactcatcg ttcatggttt
     1561 tcccaacaag gaaatcgccg ctcgcttggc agtctccaat gagaccgtcc gctggcattt
     1621 gaaccatatc tatagaaagc tccaggtcca ttcccggact gaggcagctt tgaaattccg
     1681 tcctcacaaa taaccggcca aggcggtggg cgctgcggaa cacttcggcc tcatttccac
     1741 aaattcccca ggtccggaga aaacacacca tttggtaggc taggcaggcg agtgcccgcc
     1801 cgggtaaagt gcgccgtaat caagaccaca aagatatgat gcgcctgcgc ccagccaaaa
     1861 tgccgaacgg ttttacgctt atcgaactat tggtggtgat agccatcatc gccatcctgg
     1921 cagcgatgct tttgcctgcg ctcaacaagg caaaagtcag ggcgacaatg gccacgtgtg
     1981 tcagcaacca gaagcaactc acggtggctt ggatcatgta cgccgatgac aaccaggaca
     2041 atttaatcgg cacccaaacc cccgccttca gcgccgggcc gggttattgg cgttacagca
     2101 gttataatcc tctcgagttg ggctccgtta cggggatgtc ggcgcaggat attcatattc
     2161 gtgaacttca actggcttac aaagatgcct cgctttatca atacgctccg aacttgagcg
     2221 tcattcattg cccgagcgat aggagatatg acagtccggt gggtgcgaac ttgaccacga
     2281 ccccatcgtc cgctccgggc tattttgcat tcggcagtta cgccggcgtc ggttcgctca
     2341 atggcacttt gtcgcaaatc acaaagctga cggcaatcaa acatccaacc gatcgtttta
     2401 tctgggtgga ggaaaacgat ccgcgtggcg aatgcaacgg ctcctgggac cagagttatt
     2461 ttacagcgcc accgggcttt gcggggtcaa agttggaaga ctccaccgcc tcatggcacg
     2521 tccgcagcag cacctttggc tggtgcgatg gtcatgctga aagccatggc tggatcgatg
     2581 gggccaacat cgcgtatgcg ctgaacatgg acccgaacaa gtacacgtcc tcatccgcaa
     2641 ccccatccac gggaaacagt ccccacgacc tttatttttt ggcaaacgga tgggcaacga
     2701 aagcgaaccc ttaattcatg aagaaaaccc tgacaaatat gaaaacacaa aaaaaggcat
     2761 tctgccctgg cacgatactt ttgaatgccg tggcgctggc atttctgctt accgccaatg
     2821 cctgggcggc ggattaccaa agtaccgtgc tggcggatca tccgataggt tattggccgc
     2881 taaatctggc cgtagatacc gggggcaccg cggctgacct ttccggcaat accaatatcg
     2941 ggacctatga tggcgttttt gcccaagtcc ccggcccttc gtcgttcatt acgaatgccg
     3001 tcagcttcga cggcgcaacg acgttggtcg atttgagcgc cggcagccac accgatttgc
     3061 tgaattttgg cgggacaatc acgatggaag cctgggtaca gccggcaacg cccgggatct
     3121 cgggggacat tttggcgaaa ggctacgatt ccagccagac ggatgatgaa ctcgaaatga
     3181 gggccgattc cggagactat catggcggaa cttacaacga tgacgcgggc gacctcggcg
     3241 tttccggtgg gaccgagagt accaattgga ctcacgtggt ctgtacttgg gacggaacca
     3301 attggaacct gtatgtgaac ggagaactga cgggtacagg cgcggatgca aacggggcca
     3361 ttaatttcaa cgatgcctgg gccattggga ccggcaccgc aaacggcatc agccgattgt
     3421 tcgctggtaa catctgccag gtggcgcttt acaccaatgc attgactccg gcacaagtgc
     3481 tgacccacta ccttattgga ctctatggaa caaccacccc gttgccggcc attatcactc
     3541 agccggttcc gcatacggtc tacaccaatg cttctgcaac atttagtgtc acagcggatc
     3601 ccttgagtgg gccttttggt tatcaatggt ataaaggcgc aagcgccatt tccgaccaga
     3661 cgaatgcatc tttgatgctg acgaatgttc aagctgccgc cgtggccggt tatagcgtgg
     3721 ttctcaccaa tacttacgga gcaagcacaa gtagcgtggc ggcattgacg ctcgtgccgc
     3781 cggcgaccca ctacgacgca atggtatttg gtgatcatcc cctcgcct
//
LOCUS       DASZMM010000090         5669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_850056, whole genome shotgun sequence.
ACCESSION   DASZMM010000090 DASZMM010000000
VERSION     DASZMM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850056"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..946
                     /locus_tag="VG754_02300"
     CDS             215..946
                     /locus_tag="VG754_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HWF18064.1"
                     /translation="MKKLLTWTAVVLAVAFTTSTAMAQRAYGIDVYEGNGSINWTSVN
                     NAGAHFAWAKATEGTYYKDANFWGNMNSGKNAGVIMGAYDYVRPDLDSPSAEASYFYN
                     VAGPWITTRLSAAPALDLEVFNGVVGASSYTAWCNAWFNSVKSYVSAKGFKIFNPVIY
                     ISACNACELSGSGGGFPWIASYNGENSETGSPWNVCTSCEHWGSNGWDVWQFTDTAHV
                     SGVSGYCDEDVFHGSNLAGSYLQIQ"
     gene            complement(1118..1192)
                     /locus_tag="VG754_02305"
     tRNA            complement(1118..1192)
                     /locus_tag="VG754_02305"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1157..1159),aa:Glu,seq:ttc)
     gene            1433..1597
                     /locus_tag="VG754_02310"
     CDS             1433..1597
                     /locus_tag="VG754_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18065.1"
                     /translation="MDIKTRILRWEHSIQDNPAVLALGIIVALAVVFIGAIYLDVYLK
                     ARKRKKRKRG"
     gene            1631..2872
                     /locus_tag="VG754_02315"
     CDS             1631..2872
                     /locus_tag="VG754_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWF18066.1"
                     /translation="MQSPEDASSPVGLNRRSFLKAAGLIGLSGMLPGLSRAQDDSITL
                     PFENGERPLVAYPQKRKLIRLTARPPQLETPFSVFNEGVITPNDAFFVRYHLAGIPTS
                     IDGEAFRVKIQGKVSKPLEFSIADLKAQFEPVEIVAVNQCSGNSRGFFKPRVPGGQSG
                     NGAMGNARWKGVRLKDVLAKCGVAEGARQASFNGLDFPVIQQTPDFIKALDIDHALDG
                     EVMLAYEMNGEDLPWLNGYPLRLVVPGYYGTYWVKHVNEITVMDQDLMNFWMATAYRI
                     PDNACACVEPGSKPGKTIPINRFNVRSFITSLADGAKLKSGEQVTVRGIAFDGGYGVK
                     EVLFSEDGGGNWRDADLGKDLGKYSFREWTIPFTPKQAGNFDLKVKATNRIGQSQPME
                     PLWNPSGYMRNVVETVKVSVT"
     gene            2888..3253
                     /locus_tag="VG754_02320"
     CDS             2888..3253
                     /locus_tag="VG754_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18067.1"
                     /translation="MKSNYFETKQICRKIFGVLAVALCLVGFVLVIRAKEFDMPPDTQ
                     TLKPGPGVDSAAMCTSCHSPDYILFQPRFTRTVWKAEVTKMIQKYAAPIPTNNVDQIV
                     NYLTVTYGKEDATNAAAAK"
     gene            3299..3517
                     /locus_tag="VG754_02325"
     CDS             3299..3517
                     /locus_tag="VG754_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18068.1"
                     /translation="MNVENIRKRLHEDFSPFIIRLTDGRKFSIPHHDFIALGKHAVVV
                     IDNEGYAVNIDPLHVVSLDDMRAKNGRK"
     gene            3564..>5669
                     /locus_tag="VG754_02330"
     CDS             3564..>5669
                     /locus_tag="VG754_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HWF18069.1"
                     /translation="MFDDKDVFPAAENAIFIHGAREHNLKNLSLSIPRNQFVVITGVS
                     GSGKSTLAFDLLFAEGQRRFLDSMNVYARQFVEQLSRPDVDLITGIPPTVSIEQRNSR
                     GGGKSTVATVTEIYHFIRLLFARLGTQYCPDCQLPVEAQTRDALHRRLKEETKRRGDL
                     LLLAPVVRNRKGFHTDVAEWAAKHGYAELRADGKIYQTNESFRLDRFREHDVEIIVGV
                     LEKSASVRRKSKKPTGLRTELRTPQALIDETLKLGQGTLFALDNHGKISVHSTERACP
                     KCGRSFQPLDPKIFSYNSSQGWCPKCRGFGELFYLPDVDRGARADAIEESWYEWQEGE
                     RELCPECEGARLNPIARAVRLVISPKLKPNVDSFATMPVEAAQKFFQNIKFRGREAMI
                     ARDILPEISERLKFLSEVGLGYLQMGRGVPTLSGGETQRIRLAAQLGSNLSGVLYVLD
                     EPTIGLHARDNEQLLAALQSLKARGNSVVVVEHDEETMRRADHIIDLGPGAGVNGGNV
                     VASGTLDELMRHPDSITGQCLRAKKSFPARGERRPVSPHKKDKNAWLTLRNASVHNLK
                     KLNVRFPLNRLVVVTGVSGSGKSTLVRECLLPAVQSAVKNRRSKIKNPNLSGDEFIQA
                     VYEVDQSPIGRTPRSIPATYVGFFDVIRQLFAQIPEARLRGYSPSRFSFNSAQGRCPE
                     CEGAGTIKLEMNFLPPAFVR"
BASE COUNT         1309 a         1575 c         1522 g         1263 t
ORIGIN      
        1 ttcaaaccga gttgaaggta tgatggcaaa aaaggatgga ttgttcccaa aaaccgcgcg
       61 ctttttcggt ccggcacttg ccgcatacaa cgaatgtgta ttttgacgcg cgcccttgaa
      121 agttgctagc ctgcgcccga cccagttgca ccaatgcact gagccatcaa aagacccaca
      181 aaaaccacga aaaccacaaa aggaaaaccc acctatgaaa aaactgctca catggacggc
      241 tgtcgttctg gccgtcgcgt tcacaacctc cacggcaatg gcgcaacgcg cttatggcat
      301 cgacgtttac gaaggcaacg gttcgatcaa ctggacgtct gtaaataacg ccggggccca
      361 tttcgcatgg gccaaagcga ccgaagggac atactacaag gatgcaaatt tttggggcaa
      421 catgaattcg ggaaaaaacg ccggtgttat catgggcgcg tatgattatg tgcgtcctga
      481 tctggacagc ccctccgccg aggcgagtta tttctataat gttgccggac cgtggatcac
      541 caccagattg tccgcggctc ccgcgctcga tttggaggtc ttcaatggag tcgtcggcgc
      601 ttccagttac actgcctggt gcaatgcctg gttcaatagc gtcaagtctt atgtctccgc
      661 gaaaggattc aaaatcttca atcctgtgat ctatatcagc gcgtgcaatg cctgcgaatt
      721 aagcggttcc ggcgggggct tcccctggat cgccagctat aatggcgaga actcggaaac
      781 cggcagtcct tggaacgtgt gcaccagttg cgaacactgg ggttccaacg gatgggatgt
      841 gtggcagttc acagacaccg cccatgtctc cggcgtctcc ggatattgcg acgaggatgt
      901 gttccacggg tcgaacttgg ccggctccta tctgcaaatc caatagttcg ctgcgcgcaa
      961 gtaagaaccg gtccgagctt cacgcccggg ccggttttca catttcattg caaacatttg
     1021 tgctcggatt ggggggaatg ataatgattt tttcatcgtg gttggttccg aatttgtgtt
     1081 gaaatcaacc tcctgctgag aaaacccttc tgaacgttgg cgaccctaac gggaatcgaa
     1141 cccgtgccgc cgccgtgaaa gggcggtgtc ctaaccgcta gacgataggg tcgttaacgg
     1201 gaggtgaaat ataagcccca gccacgcatt gtcacgaaaa attattaatc tcagattgtc
     1261 gcgggcgcat ccgcgcatcg gctccaaatg gatgtcaaag gcgtcacgcc tgccgccgtt
     1321 gcgcgaaggc ccatcctccg caccgttttt gcatggttaa ggtgcaacgc tgtcttttct
     1381 tcccattgac gctcgcaccc atcccggcta caactttccc aatggctgcc ccatggatat
     1441 aaaaacgcgc atcttgcgct gggagcattc gatacaggac aacccggctg tcctggcctt
     1501 ggggataatt gtggcgcttg ccgtggtttt tatcggggcc atttatcttg acgtttatct
     1561 gaaagcacga aaaagaaaga aacgcaagcg cggttgacca cccgcccgtt ccattcgaag
     1621 caaaacctct atgcaatcac ctgaagacgc atcctcccct gttggtttaa atcgccgcag
     1681 ttttctgaaa gccgccggtc tgattggttt gtcgggcatg ttgccgggcc tgtcgcgcgc
     1741 gcaagatgac tcgatcaccc tgcccttcga gaatggcgaa cgtccgctgg tggcctatcc
     1801 gcagaagcgc aaactcatcc gactgaccgc gcgtccgccg caactggaaa cccccttctc
     1861 cgttttcaat gaaggtgtca tcactcccaa cgacgccttc ttcgtccgtt accatctcgc
     1921 cggcattccg acttcgattg acggtgaagc gttccgcgtg aaaattcagg gcaaagtttc
     1981 caagcctctc gaattttcca tcgcggattt gaaagcgcag ttcgagccgg ttgaaatcgt
     2041 cgcggtgaac caatgctcgg gcaacagtcg cggttttttc aagccgcgcg tgccgggcgg
     2101 gcagtcgggc aatggcgcga tgggcaatgc gcgttggaaa ggcgtccggc tcaaggacgt
     2161 tctcgccaag tgcggcgtcg ctgaaggagc gcgccaggcg agtttcaacg gattggattt
     2221 tccagtgatt cagcaaactc cggatttcat caaagcgctc gatattgacc atgcgttgga
     2281 tggcgaagtg atgctcgctt acgaaatgaa cggcgaagat ttgccgtggc tcaatggcta
     2341 tccgctccgg ctcgttgtgc cgggatatta cggaacgtat tgggtgaagc atgtgaacga
     2401 aatcaccgtc atggaccagg atttgatgaa tttctggatg gccacagcgt atcgtattcc
     2461 cgacaatgct tgtgcgtgcg tcgagccggg ctccaagccc ggtaaaacca ttccgatcaa
     2521 tcgctttaat gtccgctcat tcatcacgag ccttgccgat ggcgcaaaat tgaagtccgg
     2581 cgagcaagtg accgttcgcg gcatcgcatt cgacggcggc tacggcgtca aggaagtttt
     2641 gttttccgaa gatggtggcg gcaactggcg cgacgctgat ttaggcaagg acctcggcaa
     2701 atattccttc cgcgaatgga cgatcccgtt cacgcccaaa caagccggca atttcgactt
     2761 gaaagtgaag gcgaccaacc gcatcggcca gtcgcaaccg atggaaccgc tttggaaccc
     2821 ttccggctac atgcgcaatg tggtcgaaac cgtcaaagtc agcgtcacgt aaaaaccacc
     2881 ttcaaaaatg aaatccaatt atttcgaaac caaacaaatc tgccggaaaa ttttcggtgt
     2941 tctggccgtt gcgctttgtc tcgttggatt tgtgcttgtc atccgcgcaa aggaattcga
     3001 catgccgccc gacacgcaga ctttgaaacc cggtcccggc gtggattccg cggcgatgtg
     3061 tacttcgtgt cattcccccg attacatcct gttccagcca cgcttcaccc gcaccgtttg
     3121 gaaggccgaa gtcaccaaga tgatccaaaa atacgccgcg ccgatcccga ccaacaatgt
     3181 tgaccagatc gtgaattatc tcacggtgac ttacgggaag gaagatgcca ccaatgcggc
     3241 ggccgccaaa tagactggcg agcttgacta aattgcaatt tgacggcaaa gaaatcgcat
     3301 gaacgtcgaa aatattcgca aacggctgca cgaggatttc agccctttca tcatccggct
     3361 tacagatggc cgaaaatttt cgattccaca tcacgatttc attgccctag gaaaacatgc
     3421 ggtggttgtc atagacaatg aaggctatgc agtaaatatt gatcctttgc atgttgtttc
     3481 cctggatgac atgcgtgcaa agaacggcag aaaataaaaa ctgccgactt tgccattcgc
     3541 tcgtgtgatg ttagtttgca agcgtgtttg acgacaaaga tgtttttcca gcggcagaga
     3601 acgcaatttt catccatggc gcgcgcgagc ataatctaaa aaatctctcg ctcagcatcc
     3661 cgcgcaatca attcgtcgtc atcaccggag tcagcggctc gggcaaaagc acgctggcct
     3721 ttgatttgct tttcgcggag ggacaacgcc gtttcctcga ttcgatgaac gtctatgcgc
     3781 ggcaattcgt cgagcagctt tcccgcccag acgtggactt gatcaccggc attccgccga
     3841 cggtcagcat cgaacagcgc aacagccgcg gcggcggcaa aagcaccgtc gcgacggtga
     3901 cggaaatcta tcattttatc cggctccttt tcgcgcggct cggcacacag tattgtcccg
     3961 attgccagtt gcccgtcgaa gcgcaaacgc gcgacgcact ccaccgccgg ctcaaggagg
     4021 aaacgaaaag gcgcggcgat cttttgctgc tcgcgccggt cgtgcgcaat cgcaagggct
     4081 ttcacacgga tgtcgccgaa tgggctgcga agcatggtta cgccgagttg cgcgcggatg
     4141 gaaaaattta tcaaacgaac gaatctttcc gcctcgaccg tttccgcgag catgacgtgg
     4201 agatcatcgt tggagtgctt gaaaaatcgg cgtctgttcg ccggaaatcg aaaaagccca
     4261 ccggtctaag gacagaactg cggacaccgc aggcattgat tgatgagacg ttgaagcttg
     4321 gccaggggac gctgttcgct ctggacaatc acggcaaaat ttccgtgcat tcaacggagc
     4381 gcgcctgccc caaatgcggt cgttcgtttc agccattgga tcccaaaatt ttcagctaca
     4441 attcctcaca gggctggtgc cccaaatgcc gcgggttcgg cgaattattt tatctgcccg
     4501 atgtggaccg cggcgcgcgc gccgatgcca tcgaggaatc gtggtatgaa tggcaggaag
     4561 gtgagcgcga gctttgcccc gaatgcgaag gggcgcggtt gaatcccatc gcccgcgccg
     4621 tgcgactggt catcagccca aaattgaaac cgaatgttga ttcattcgcg acgatgccgg
     4681 ttgaagccgc gcaaaagttt tttcaaaaca ttaaattcag gggccgcgaa gcgatgattg
     4741 cgcgtgacat ccttccggaa atcagcgagc gactgaaatt tttgtctgag gttggcttgg
     4801 gttatctgca aatggggcgc ggtgtgccga ccttgtccgg cggcgagacg caacgcatcc
     4861 ggctcgcggc gcaactcggc tcgaatctca gtggcgtgct ttatgtgctg gatgagccaa
     4921 ccatcggcct gcacgcgcgc gacaacgagc aactgctcgc ggcattgcaa tcgttgaagg
     4981 cgcgcggcaa ttccgtggtc gtggtcgagc atgatgagga aacaatgcgc cgcgccgatc
     5041 acatcattga ccttggaccg ggcgcgggtg tcaacggcgg caacgtcgtt gccagcggaa
     5101 cgctggacga attgatgcgc catcccgatt ccatcaccgg ccaatgtttg cgcgccaaga
     5161 aaagttttcc ggcgcgaggc gagcgccggc cagtttcacc gcacaagaaa gataaaaacg
     5221 catggctcac tcttcgcaat gcgtcggtcc acaatttgaa aaaattgaac gtccggtttc
     5281 cattgaaccg tctggtcgtc gtcaccggtg tcagcggttc cggcaaaagc acgctcgtcc
     5341 gcgaatgcct tttgcccgcc gtccaatccg ccgtcaaaaa tcgcagatca aaaatcaaaa
     5401 atccgaatct ttccggcgat gaattcatcc aggccgttta tgaagtggac caatcgccca
     5461 ttggccgcac gccgcgctcg attcccgcca cttatgtcgg ctttttcgat gtcatccggc
     5521 aattatttgc gcagattccc gaagcgcggt tgcgcggata ttcgccgagc cggttttcat
     5581 tcaacagcgc gcaaggccga tgccccgaat gcgaaggcgc aggcaccatc aagctcgaaa
     5641 tgaattttct gccgcccgcg ttcgtgcgc
//
LOCUS       DASZMM010000091         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4161595, whole genome shotgun sequence.
ACCESSION   DASZMM010000091 DASZMM010000000
VERSION     DASZMM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4161595"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VG754_02335"
     CDS             complement(<1..735)
                     /locus_tag="VG754_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWF18070.1"
                     /translation="MVFAKKTNGGIVVSLIIAVFLWGGSNAGTKYVVKVWGPGWIGST
                     RLLCAGLCLLAILKWTRWLGTLTPLTAATKRELWLRGGLSLAVYIVVFNWALHFTPAS
                     HVALYLGMSPIWTLLWEERPTMTLRSVQRYGAAGLALTGVVVLFWPSLRTGGTGAMGE
                     VLGFLASWLWANYGRQIRALGGKFSGAEISAHTMWRAGLLLLPLAIVEIAKSGLVWRT
                     DLVLVQGYCIIAGGVIAYVLWNNALRH"
     gene            813..1187
                     /locus_tag="VG754_02340"
     CDS             813..1187
                     /locus_tag="VG754_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWF18071.1"
                     /translation="MDHAPGFLKLVNEARKEVKEISIEQARERLAKNPNAVLLDVRED
                     LEWQKGRAAQAIHLGKGILERDLEQKFPDPDTELIMYCGGGYRSILTAQSAQNMGYRN
                     VSSLVGGYKGLVQAQWPMKSGD"
     gene            complement(1154..2806)
                     /locus_tag="VG754_02345"
     CDS             complement(1154..2806)
                     /locus_tag="VG754_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HWF18072.1"
                     /translation="MKLFKFLFQSCRRMLFLTTVAALLSGACNAGLIALVNRGLNRNG
                     MQLRTLAMWFVVLVLGKVGSQLLSQGLLAKFAQKSISELRYDLIRRILSVPLRRLEEI
                     GSPAILVALTDDVFNIAQALLAIPLTSVNIALVFGGAVYLGWLSWKLLATMMIFMVLG
                     GISYRLLIGSAYKYLHLAREEESRLYRHFRSLTEGIKELKLHRNRRVNFMREHVWESA
                     EAFQNHNVRAEYRFVLAQNWNYILLYVLVGLLIFVLPGVQPISTPTLTGYVLMILFLM
                     GPLAGVMSSFSLFGRAEVALEKVEKLGGTLASYEAERDALPKTETKEFIEELELVNVI
                     HTYRTDSDERDFVLGPVNLQFGPSELVFLVGGNGSGKSTLAKIITGLYFPEGGEIRLN
                     GKVVDHHNRDEYRQIFSAVFADFYLFDTLMGLNGSHLDEQAQEYLEELHLHRKVKIAN
                     GALSTTSLSQGQRKRLALLTAYLEDRPFYLFDEWASDQDPQFKRVFYTQLLPELKARG
                     KTVLVITHDDQYFHLADRIIKLDYGQVVTHAAQSPDFIGHCA"
     gene            complement(2803..>4188)
                     /locus_tag="VG754_02350"
     CDS             complement(2803..>4188)
                     /locus_tag="VG754_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF18073.1"
                     /translation="FRQGTSGGYVYRLPAEFAAGLAPDSGFVEQKRVVDVPEKTTLAT
                     KAPTRRKFNRYGRIARELNNAEGILTALDAKFRLHSHDAHPIAPPVTDTEKLLCKIWQ
                     DLLRRDQIGVRDNFFELGGHSLLAVRLFSEIERLTGRKLPLVTVFQSPTIRELADVLS
                     EQATSSRSCLVAIHAQGTRRPLFLIHGAGGDVLWGYANLAPYLGDDQPVYGVKSRALD
                     GSEEFATIEEMASHYIHQIRSLQPEGPYLLGGYCFGGNVAYEMARQLVEQGEKVELLA
                     LLDSAPSNTGYETMPWWRPVFVFKFAVNLCYWLEDFFRWKPEDRREFVKRKSRAWGRK
                     FTRVLSHNRKKAGDVDLEEVIDLTKFPPHELRLWQIHLNILVRHVSQPYAGKVVLFRT
                     RGQPLFCSLEEDFGWKRLVRGGVDILMTSGEHESIFIEPNVRELAWLLRNCLDTANKT
                     RQPAMTELKPT"
BASE COUNT         1009 a         1208 c         1110 g          861 t
ORIGIN      
        1 gtgccgcagc gcattgttcc acagcacata agcgatcacg ccgcctgcga tgatgcaata
       61 cccctgcacc agcacgagat cagttcgcca aaccaagccc gacttcgcga tttccacgat
      121 tgccagcggc agcaacaaca atcccgcccg ccacatcgta tgcgcgctga tttccgcgcc
      181 ggaaaatttc ccgcccaatg cccggatttg ccgcccgtaa ttggcccata accagctcgc
      241 cagaaaacca agcacttcgc ccatcgcacc ggtgccgccc gttcttaacg agggccaaaa
      301 caaaaccacc acgcccgtca acgccagtcc cgccgctccg taacgttgca ccgagcgcaa
      361 agtcatcgtc ggccgttctt cccacaagag ggtccaaatc ggcgacattc ccaaatacaa
      421 cgcgacgtgc gaagccggcg tgaagtgcag cgcccagttg aacaccacga tgtaaacggc
      481 cagacttaga ccgccgcgca gccataattc gcgtttggtc gcagcggtga gcggggtcag
      541 cgtgcccagc cagcgcgtcc atttcaaaat ggcgagcagg cacaatcctg cgcagagcaa
      601 gcgcgtgctc ccgatccagc cggggcccca gactttcacc acgtatttcg tgccggcatt
      661 gctgccaccc cataaaaaaa ccgcgatgat cagtgagacg acgatgccgc cgttggtttt
      721 ctttgcgaac accattcaag taagccggga gcgcagagtg gaagcaattg aatttcattt
      781 gaccgcacgg attgcatgat taaattcctc ccatggatca cgcacccggt tttcttaaat
      841 tggtcaacga agcgcgcaag gaagtcaaag aaatcagcat cgaacaggcg cgcgaacgtc
      901 ttgcgaaaaa tccaaacgcc gtcctgctgg acgtgcgcga ggatttggaa tggcagaagg
      961 gccgcgccgc gcaggccatt catctcggca aaggaatcct tgaacgggac ctggagcaga
     1021 aatttcctga tcccgatact gaactgatca tgtattgcgg cggcggttat cgttccatct
     1081 tgacggcgca aagcgcgcaa aacatgggtt accggaacgt cagctcgctg gttggcggct
     1141 acaaaggcct ggttcaggcg cagtggccga tgaaatccgg cgattgagcg gcgtgtgtca
     1201 cgacctggcc gtaatccaat ttgatgatgc ggtcggcgag atggaaatat tggtcgtcat
     1261 gggttatcac aaggacggtt ttgccacgcg ccttcaattc cggcaacagt tgtgtgtaga
     1321 acacccgttt gaattgcggg tcctggtcag aagcccattc gtcgaaaaga taaaacggac
     1381 ggtcttccag ataggcggtg agcagagcca aacgcttgcg ttgcccttgg gaaagcgatg
     1441 ttgtcgagag agcgccgttg gcgatcttta ccttgcggtg cagatgaagt tcctcgagat
     1501 attcctgcgc ctgctcatcc aaatggcttc cgttcaggcc catgagcgtg tcaaaaagat
     1561 aaaaatccgc aaacaccgcc gagaaaatct gccgatattc gtcgcgattg tggtggtcca
     1621 ccactttgcc gttcaaacgg atttcgcccc cttccgggaa ataaagcccg gtgatgattt
     1681 ttgcgagtgt ggatttgccg ctgccgttgc cgccgacgag aaacaccagt tcgctcggcc
     1741 caaactgcaa attgacaggg ccgagcacga aatctctttc atcgctgtcg gtgcgataag
     1801 tgtgaatcac gttgaccaat tcgagttcct cgatgaattc ctttgtttcc gtcttcggca
     1861 aggcgtcccg ctcagcttca tacgaagcga gtgtcccgcc gagtttctca actttttcca
     1921 aggcgacttc cgcgcggcca aaaagcgaaa aactgctcat cacccccgcg agcggtccca
     1981 tcaaaaaaag aatcatgagg acatagccgg taagcgtcgg agtgctgatc ggttggacgc
     2041 cgggcagcac aaaaatgagc aatcccacca aaacgtagag caatatgtaa ttccagtttt
     2101 gcgccaaaac aaaacggtat tcggcgcgga cattatggtt ttgaaatgct tcagcgcttt
     2161 cccagacatg ctcgcgcatg aaattgacgc gccggttgcg atgcagcttg agttccttga
     2221 taccttcggt gagcgagcga aaatggcggt aaaggcgcga ttcctcttcg cgggcaagat
     2281 gcaaatattt gtacgcgctg ccgatgagca gcctataact gatgccgccc aaaaccatga
     2341 aaatcatcat ggtcgcgagc agcttccatg aaagccagcc gagatatacc gccccgccaa
     2401 agacaagcgc aatgttcacg ctggtgagtg gaatcgccaa cagcgcctgg gcgatgttga
     2461 acacgtcgtc ggtgagggcg acaagaatcg cgggactgcc gatttcctcc agccggcgaa
     2521 gcggcacgga aagaatcctg cggatgagat cgtatcgcag ttcgctgatg gatttctgcg
     2581 cgaactttgc cagcaaacct tgtgacagca attgcgaacc gaccttgccc aataccagaa
     2641 ccacgaacca catggcaagt gtgcgcaact gcatcccgtt ccggttcagg ccccggttca
     2701 ccaaggcgat cagcccggcg ttgcacgcac cgctgagcag cgccgccacg gtcgtcaaaa
     2761 acaacatcct tcggcaagat tggaacagga atttgaatag tttcatgtag gtttgagttc
     2821 tgtcatcgcc ggctggcgag ttttgtttgc ggtgtcgaga caatttctga gcaaccaggc
     2881 cagctcacgg acatttggct cgataaaaat gctctcgtgt tcgccggagg tcatcaggat
     2941 gtccactccg ccgcgcacga gccgtttcca gccaaaatct tcctccagcg agcagaacaa
     3001 tggctggcca cgcgtgcgga agagcacgac cttgccggca tacggttgcg acacgtgacg
     3061 caccaaaata ttgagatgaa tctgccaaag ccgcagttca tgcggaggga atttggtcaa
     3121 atcaatcacc tcttccagat ccacgtcccc tgctttcttc cgattgtggg aaaggactcg
     3181 ggtgaatttg cggccccaag cccggctttt gcgcttcacg aattcccggc gatcttcggg
     3241 cttccagcgg aaaaagtcct cgagccaata acaaaggttt acggcaaatt tgaaaacgaa
     3301 cacgggacgc caccagggca tggtttcata accggtgttg gacggcgcgc tgtcgagcaa
     3361 cgcaagaagt tcaacctttt cgccctgttc aaccagttgc cgcgccattt cgtaggcaac
     3421 attcccgccg aaacaataac cgccaagaag atacggcccc tcgggctgca acgatcggat
     3481 ttgatggatg taatgcgatg ccatctcctc aatggtcgcg aattcctcgg aaccatcgag
     3541 agcgcgcgat ttcacgccgt acaccggctg gtcgtcgcca agatacggcg ccaagtttgc
     3601 ataaccccaa aggacatccc caccggcgcc atggatgaga aacaaagggc gccgcgtgcc
     3661 ttgagcatga atggccacca agcaagagcg cgaactggtt gcctgctccg aaagcacatc
     3721 ggcgagttcc ctgatggtcg ggctttgaaa tacggtcacc agcggaagtt ttcggcctgt
     3781 gagccgctca atctcggaaa aaagccttac ggccaacaac gaatgccctc ccagttcaaa
     3841 gaagttgtcc ctcactccaa tttgatcgcg acgcaacaag tcctgccaaa ttttgcacag
     3901 gagtttttct gtgtcggtta ctggcggcgc aatcgggtgt gcatcgtgcg agtgcaggcg
     3961 gaattttgcg tccaacgcgg tgagaatgcc ctcggcattg ttcaattcac gcgcaatgcg
     4021 accgtagcga ttgaattttc ttcgcgtcgg cgccttggtt gccaaagtgg tcttctcggg
     4081 gacatccaca acgcgcttct gttccacgaa cccggaatct ggcgcaaggc ctgcggcgaa
     4141 ctccgccggc aaacggtaaa catatcctcc gcttgttccc tgacggaa
//
LOCUS       DASZMM010000092         6846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4161748, whole genome shotgun sequence.
ACCESSION   DASZMM010000092 DASZMM010000000
VERSION     DASZMM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4161748"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VG754_02355"
     CDS             complement(<1..671)
                     /locus_tag="VG754_02355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWF18074.1"
                     /translation="MSSRTEIVTVEASRPSERLDVYLRGIFPVVSRGTIQRLIEQGHI
                     RVNGQTVKATHHPCRGEQIEVHWPEARPAEAQPEKMELDILFEDENLLVLNKPPGLVV
                     HPAAGHEEHTLVNALLHHCAGQLSGIGGVARPGIVHRLDKETSGCMVVAKNDAAHLGL
                     AEQFAGRTLEKIYHAIVCGEVPRESGEIRAAIARHPTHRKRMAVTDGSGREAWTSYRV
                     LERLRG"
     gene            complement(827..1345)
                     /gene="lspA"
                     /locus_tag="VG754_02360"
     CDS             complement(827..1345)
                     /gene="lspA"
                     /locus_tag="VG754_02360"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWF18075.1"
                     /translation="MDSKRMLSTENGRIAIVAVIVLVLDQLTKQLVNRLLPMETDEKI
                     LIPGFFKFVHWGNTGAAWSLFSGNNGFLAIVALIALFILFRSRHHFDAHTLMGQIALG
                     LIFGGIIGNLTDRLRVGHVTDFLYFHLQRRGGPDIGFPAFNIADSAICIGVGLIFILS
                     LRSEKTPIASTS"
     gene            complement(1379..2029)
                     /locus_tag="VG754_02365"
     CDS             complement(1379..2029)
                     /locus_tag="VG754_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HWF18076.1"
                     /translation="METTYTMIGADGLQYGPINLGQIRSWIGEGRITAETKILRSDTN
                     AWLPASQYAELGLAQPQLQTRGPAVAVASPSMPVSGPNPALERRARSGARWFFWIAGL
                     SLINSFMAMSGKAVVFVVGLGVTNLVDLTVQQMGANMTIALVINLVISGVFALFGIFA
                     MQRRSWSFIVGMFLYGLDAVLAYMVAGWLGLGFHLFVLFWIYQGLRASMELKTQGA"
     gene            complement(2149..4248)
                     /locus_tag="VG754_02370"
     CDS             complement(2149..4248)
                     /locus_tag="VG754_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18077.1"
                     /translation="MDNTVFAQGTAFTYQGSLLSNGQQVSGGYDMTFSVFNSASNGVP
                     VAGPVTNLAIVVSNSLFTTTLDFGSGVFSGSSYWLEIAVRTNGNGGFTKLNPRQALTP
                     TPYAIFSANAATATSAASVAATNITGALPTTALPPAVLTNGAGGVTISGTFSGNGNGL
                     NNLNAGSLAGVVQTNNLPGNLASLAQNNGGQLTNLYHRIYNVRDFGAGNGNPNQDSAA
                     ILAAFNAWTNTGGVLYFPPGEYYDTNQYVIGPFAGNDNFEDSALVIRGGGSPCTIWHW
                     FGINETMITCNTSGGLQPVVIEGITFICGHSVANFGPSEGNAGTNNCIIQSGDSGHVM
                     WTDVRFENWMGYGVIEDSSAGGRYYGMQFLWCGTGISLFGYDDGTFIQCIGYSCTNAV
                     VETGRAATWSGSGGNKGVNGLNVNIFGATDCAGVIVGNGGGASVSGYMEGCTNGILQI
                     GHADGSDTSIGPCSIQNCGGQRLCVGIFSPSVLFTQRNCEGWGSGNTNIICFTSAADS
                     TPMILEGRSGSVFFSDGSTLNLTSSVVYVNASLAEYNKIAGSVSNVLGYTTNSFYYHT
                     PKASLDTNGMMTSQSYIGGRNLGTISSGTYNVNLGLKYQDVNVTTNITVALTNWAIFN
                     ATNAADVKVLFYPGSGNPGISFSSIGNLWWSSESGTATAPTSIPSGKILIVDFTVEVN
                     LSSTNIIGHYSFAPYTP"
     gene            4525..4959
                     /gene="rplM"
                     /locus_tag="VG754_02375"
     CDS             4525..4959
                     /gene="rplM"
                     /locus_tag="VG754_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWF18078.1"
                     /translation="MKTHLPKINLDQRKWHVIDANGAILGRLAVQIADTLRGKNKPIY
                     TPHLDAGDFVVVINAEKIAVTGKKETEKLFMTYSGWKGGERYKSVAQVRAKHPEQLIT
                     HAVKGMVPKNRLGRVLMTKLKVYKGSAHPHAAQQPATLAPIK"
     gene            4982..5377
                     /gene="rpsI"
                     /locus_tag="VG754_02380"
     CDS             4982..5377
                     /gene="rpsI"
                     /locus_tag="VG754_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWF18079.1"
                     /translation="MAATSEFLGTGRRKTSIARVRLASGSGKIVVNGRPFETYFPTET
                     LRMVVSQPLVVTSTSGKFDIRVNVQGGGPTGQAGAVRHGIARALLEADIALRPTLKSE
                     GLLTRDSREKERKKYGQPGARKRFQYSKR"
     gene            5498..6562
                     /gene="argC"
                     /locus_tag="VG754_02385"
     CDS             5498..6562
                     /gene="argC"
                     /locus_tag="VG754_02385"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWF18080.1"
                     /translation="MDKKVKVGIVGASGYSGEELVRLLLRHPNVELTALTSRQYAGQT
                     LAQVFPQFGHYPEAKSIRFSEPNASLLAKQAQVIFLALPHGVSAEFAVPLLQHGCQVI
                     DLSADFRLRNAEVYKDYYAHDHPAPELLSTAVYGLPEVYRAAIKNALLIASPGCYPTS
                     ILLPVLPLLRAGLIKPTGIIADSLSGVSGAGRKVEQDYLFVECNESVRPYGIPKHRHL
                     SEIEQELSLAAGTPVTIQFTPHLIPVNRGILTTLYLAPEKHFSTAKEAAELNEKITAC
                     YQAAYGNEPFVRLLDGKSLPDTKHVVGTNVIEIAWRLDHRTGRLLVMSAEDNIVKGAS
                     GQAVQSMNILCGFPETAGLM"
BASE COUNT         1608 a         1965 c         1724 g         1549 t
ORIGIN      
        1 ccgcgcaacc gttcaagcac gcgatagctc gtccatgcct cgcgtccgct gccatcggtc
       61 accgccatcc gcttgcgatg ggttggatgc cgcgcaatgg ccgcgcgtat ttcgccgctt
      121 tcacgcggga cttcgccgca aacaatcgcg tgataaatct tttccaaggt tcggcctgcg
      181 aattgttccg caaggccaag atgcgccgca tcgttctttg ccaccaccat gcaaccgctg
      241 gtttccttgt cgagacgatg gacaatcccc ggtcgcgcca ccccgcctat tccgctcaac
      301 tgacccgcgc aatgatgcaa caacgcattg acgagcgtat gttcctcatg gcccgccgcc
      361 ggatgcacca ccaatccggg cggcttgttg aggaccagca ggttctcgtc ctcgaacaga
      421 atgtccaact ccatcttttc gggttgcgcc tcggcgggac gcgcttccgg ccaatgcacc
      481 tcaatctgtt cgccccgaca cggatgatgc gtcgccttca ccgtctggcc gttcacgcgg
      541 atatgcccct gctctatcaa acgctggatg gttccccgcg aaaccacagg gaatattccg
      601 cgcagataaa catccaaccg ctcgctggga cgcgaagctt caactgtgac gatttcagtg
      661 cgagaagaca tttttttgat ttacgatttg cgatatgcgg ttcacgcgca aaagatttga
      721 ttttcgtgcc gcgtatttcg gcgttgatga ttcttaccaa agcgcccggt ggaagacgga
      781 aaacgcagca cgcaactgga acgcgtcgat cgtatatcgt aaattctcag gacgtggaag
      841 caatcggcgt tttttcgctt ctcagcgaga gaataaaaat caatcccacg ccgatgcaaa
      901 tggcgctgtc ggcaatgttg aatgcgggaa aaccgatgtc cggaccgccg cgccgctgca
      961 aatggaaata tagaaaatcg gttacatggc cgactctgag gcggtcagtg agattgccga
     1021 tgatgccgcc gaaaatcagc ccgagcgcga tctggcccat gagcgtgtgc gcgtcaaaat
     1081 gatgccggct gcggaacagg atgaacaagg cgatcaacgc aacgattgcc aggaacccgt
     1141 tgttacccga aaaaaggctc caagccgcgc cggtgtttcc ccaatgcaca aatttaaaga
     1201 aaccaggaat caggattttc tcgtccgtct ccatcggcaa cagacggttg acgagttgtt
     1261 tggtgagttg atcaagcacc agcacgatga ccgcgacaat ggcgatgcgc ccattttccg
     1321 tgctgagcat ccgtttggaa tccattcgtc aatataccat tttttgccgg cctgcaactc
     1381 aggcgccttg cgtcttcaac tccatgctcg cccgcagtcc ctgataaatc cagaacagca
     1441 cgaacaaatg gaaacccagc ccgagccatc ccgccaccat atacgcaagg accgcgtcca
     1501 ggccatacaa aaacattccg actatgaaac tccaactccg ccgttgcatc gcaaaaatcc
     1561 cgaacaatgc gaacactccg gaaatgacca gattgatcac cagcgcaatg gtcatgtttg
     1621 cgcccatctg ttgaacggtc aaatccacga gattggtaac gcccaatccc acgacaaata
     1681 cgacagcctt gccggacatg gccatgaacg aattgatcag cgaaagcccc gcgatccaga
     1741 aaaaccagcg cgctcccgat cgcgcccggc gttccaatgc cggattcggt ccgctgacag
     1801 gcatcgacgg actggccacg gcaacagcag gcccgcgcgt ttgcagttgc ggttgcgcga
     1861 gtccaagctc ggcgtattgg gaagctggca gccatgcatt ggtgtcgctg cgcaaaattt
     1921 tcgtctcggc ggtgatgcgg ccttcgccaa tccaactccg gatttggccc aggttgatcg
     1981 ggccgtactg caggccgtcc gcgccgatca ttgtgtaggt tgtttccata gttcgtatcg
     2041 gttccatccc ggcaggacgc gattatttag ccacctcatc gccaaatgac aacttcaaac
     2101 tggctgcgat tcaggatgca taggtttttt catgaccata ataaacggtc atggagtata
     2161 aggtgcgaac gagtaatgac caatgatatt ggtggatgac aaattgacct ccaccgtaaa
     2221 gtccacaatc aatattttgc cggacggaat gcttgtcgga gcggtcgccg tgccgctctc
     2281 actgctccac cacagattac cgatgctgga aaacgaaatg ccaggattcc ccgaaccggg
     2341 ataaaacaag accttcacgt ccgcagcgtt ggtggcattg aagatggccc agttggtcaa
     2401 cgcgaccgtg atgttggttg tgacgtttac atcctgatat ttcaatccaa gattaacatt
     2461 gtaagtcccc gaggaaatcg ttcccagatt cctgccccca atataggact gcgaagtcat
     2521 catcccattg gtgtcaagcg aggcttttgg tgtgtgataa taaaatgagt ttgtggtgta
     2581 accgaggaca ttgctgacgc ttcctgcaat cttgttgtat tccgcaagag acgcattcac
     2641 atagaccaca gagcttgtca ggttaagggt gcttccatcg ctaaagaaca cactgccaga
     2701 ccgcccttcc aatatcatcg gcgtggaatc ggcagcggaa gtgaaacaga tgatgttagt
     2761 gtttcctgac ccccatcctt cgcagtttct ttgtgtgaat aaaacagatg gcgaaaaaat
     2821 accaacacat aaacgctgtc ctccgcagtt ctgaatcgag caggggccga tgcttgtgtc
     2881 ggaaccgtca gcatgtccga tttgcaagat gccgttggtg cagccctcca tatagccgct
     2941 gacacttgcg ccaccgccat tgccaacgat tactcccgcg cagtcggtcg caccaaaaat
     3001 atttacgttc agtccgttta cccctttatt gcccccactg ccactccaag tcgccgccct
     3061 tccagtctcg acaacggcat tggtgcagga ataaccgatg cattggataa aagtaccgtc
     3121 atcgtatcca aacagtgaaa ttccagtgcc gcaccacaga aattgcatcc cgtaatatct
     3181 tcctcctgcg ctgctatcct cgattacgcc gtaacccatc caattttcaa atcgcacatc
     3241 cgtccacatg acatgtccac tgtcgccgct ctgaatgatg cagttgttag tgccggcatt
     3301 gccctcagaa gggccgaaat tcgccacgga atggccgcaa ataaaagtga ttccttcaat
     3361 gacaaccggc tgcaatccgc cggatgtatt gcaggtgatc atcgtttcat ttattccaaa
     3421 ccaatgccag atagtgcagg gcgaacctcc tccccggatg accaaggcag agtcctcaaa
     3481 attgtcattc cccgcaaatg ggccaatgac atattggttg gtgtcgtaat actcgccggg
     3541 cgggaaatag agcacgcccc cggtgttcgt ccacgcgttg aatgccgcaa ggattgccgc
     3601 gctgtcctgg tttggatttc cattgccagc gccaaaatcg cgcacgttgt aaatgcggtg
     3661 atacagattg gtcaattggc ccccgttgtt ctgcgccaat gatgcaagat tgcccggcaa
     3721 attgttcgtt tgcaccacgc ccgccaaact tcctgcattt agattgttca acccgttgcc
     3781 attgccgcta aaagttccag aaatggtcac accacccgct ccattggtca gcactgcggg
     3841 aggcaacgcg gtcgttggca acgcgccggt aatgttcgtg gcggcgacgg aagccgcgct
     3901 ggttgccgtc gctgcattcg ctgaaaagat ggcataggga gtgggtgtca gcgcctgccg
     3961 cggatttaat ttggtgaacc ccccgtttcc gtttgtgcga actgcaatct ccaaccagta
     4021 ggaactgccg gagaacacac cggatccaaa atccagcgtg gtggtaaaaa ggctattgct
     4081 gacgacgatg gcgagatttg ttaccggacc ggcgacgggc accccattcg atgcggaatt
     4141 gaacactgaa aaagtcatat catagccgcc acttacctgc tgtccattgc tcaacaagct
     4201 tccttggtac gtgaatgctg tcccttgcgc aaaaacggta ttatccattg caaggcagga
     4261 aaaaagaaac agggatcgaa aaaggaccgt ggaaagtttc attggtttta aatcagcgtg
     4321 gacagctctt atcaaagcaa agaaaaaagt cgagtgcatt ttcatgtggt gccgatttgc
     4381 gtcgggaaag ccggccaaag tttccgacaa agcgcggatt tcgcactatg cgaacggcaa
     4441 atgaggctta aaataatccc caaaacccta ttgacttaac ctgttctttt gataccttct
     4501 cccctccttt gatagggatt tgagatgaaa acgcatttgc cgaaaattaa cttggatcag
     4561 cgtaagtggc atgtgatcga tgccaacggc gccattttgg gccgcttggc cgtgcagatt
     4621 gccgatactt tgcgcggaaa aaacaagccg atctacaccc cgcacctcga cgcgggcgat
     4681 tttgtggtcg tcatcaatgc cgagaagatt gccgtcaccg gcaagaaaga gacggagaag
     4741 ctgttcatga cttattccgg ctggaagggc ggcgagcgtt acaagagcgt cgcgcaagtc
     4801 cgcgccaagc accccgaaca actcatcacc cacgccgtca agggcatggt tcccaagaac
     4861 cgcctcggcc gggtgctcat gaccaagctc aaagtttaca agggcagcgc ccatccgcac
     4921 gccgcgcagc aacccgcgac gcttgccccg atcaagtaac aattttcaat cgaattgaat
     4981 tatggcagca acttctgaat ttcttggcac cggccgtcgc aagacttcca tcgcgcgcgt
     5041 ccgcctcgct tccggctccg gcaaaatcgt cgtcaatggc cggccgtttg aaacttattt
     5101 tccgaccgaa accctccgca tggtcgtcag ccagcctttg gtggtgacct ccacgtcagg
     5161 caagttcgac atccgcgtga atgtccaagg tggcggaccc actggtcagg ccggcgcagt
     5221 ccgtcacggc atcgcccgcg cgttgctcga agcggacatc gcgttgcgtc ccacgctcaa
     5281 gtccgaaggt ttgctgaccc gcgattcgcg cgagaaggaa cgcaagaaat acggccagcc
     5341 cggcgcgcgc aaacgcttcc agtacagcaa gcgttaagcc gatttgctct tttgcacccc
     5401 gcgggcttcg gcctgcgggg ttttttgctt taacacatgg gaaacttttc catttcattt
     5461 tttctgaatt cgtggattgc tagttgttgc catcatcatg gataaaaaag tcaaagttgg
     5521 aatcgtcggc gcgtccggtt actcgggcga ggaactggtg cgccttctgt tgcggcatcc
     5581 caatgtggaa ttgaccgcgc tgacttcgcg tcaatacgcc ggccaaacgc ttgcgcaggt
     5641 ctttccccaa ttcggccatt atcccgaagc caaaagcatt cgcttcagcg agccgaacgc
     5701 ctctctgctg gctaaacagg cgcaggtgat ttttctcgcg ctgccgcatg gcgtctcggc
     5761 ggagttcgcc gttcccctgc tccagcacgg ttgccaagtc attgatctga gcgccgattt
     5821 ccggctgcgc aacgcagaag tttacaaaga ttattacgcg catgaccatc ccgcgccgga
     5881 actgctctca acagcggttt acggactgcc cgaagtttat cgtgcggcca tcaagaacgc
     5941 gctgttgatt gcgtcgccgg gttgttatcc cacgagtatt ttgctgccgg tgttgccatt
     6001 gctgcgcgcc ggtctcatca agccgaccgg catcatcgcc gattcattga gcggcgtcag
     6061 cggcgcagga cggaaggtcg aacaggatta tcttttcgtg gaatgcaatg agagcgtccg
     6121 gccttacggc attcccaagc accggcatct ttccgaaatc gagcaggaac tttcgttggc
     6181 tgcgggcacg ccagtcacta ttcaattcac tccgcatctc ataccggtga atcgcggaat
     6241 cttaacgaca ctctatctcg cgcctgaaaa acatttctca accgcgaaag aagccgccga
     6301 gttgaacgaa aaaatcaccg cttgctacca ggctgcttac ggcaatgaac cgttcgtgcg
     6361 cttgcttgat ggcaagtcgt tgccggatac aaagcatgtc gtgggtacga atgtcatcga
     6421 aatcgcctgg cgactcgatc atcgcaccgg acgcctcctc gtgatgagcg ccgaagacaa
     6481 catcgtcaaa ggcgcaagcg gccaagccgt gcaaagcatg aacatcctct gcggctttcc
     6541 cgaaactgcg gggttgatgt gaaattcaag ttgttccatc gtagcgcaga gttgcactct
     6601 gctgtatcgc aaatttttca atctgcgagg cgcgcgagtt catgccgcat ccaacaattt
     6661 ccacctcatt tttttcttgc cgcccagccg actgcaagtc ggcgatacgg cagattaaaa
     6721 atctgcgcta cttgttcggc aatggcgaaa attttttcga attgattctc ccatgaagca
     6781 cattctcatc tcaagctcca tcaccgcgcc gcaaggcttt ttggcttcgg gcgttttttg
     6841 cgacat
//
LOCUS       DASZMM010000093         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3601262, whole genome shotgun sequence.
ACCESSION   DASZMM010000093 DASZMM010000000
VERSION     DASZMM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601262"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VG754_02390"
     CDS             complement(<1..858)
                     /locus_tag="VG754_02390"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWF18081.1"
                     /translation="MNLREQMTELARQAKAASRELARLTTAEKNACLLAMADTLEHGA
                     ETIKAANAKDMAAGAQSGLSSAMLDRLKLDDKRIAAMAKGLREVAALPDPVGRILDSR
                     TRPNGLKLQKISTPIGVVVIIYESRPNVTADAASLCFKSGNATILRGGKEAIHSNQAI
                     AQVMLSAAKQRLPHFPEHAIQVVQTIDREAIKELLSLTQYVDLCMPRGGEGLIRAVTE
                     NSKVPVIKHYKGVCHVYVDGDADFKMAEEIVLNAKCQRPGVCNAIETLLVDKSIAQEF
                     LPVIAQKLAE"
     gene            complement(1192..2127)
                     /locus_tag="VG754_02395"
     CDS             complement(1192..2127)
                     /locus_tag="VG754_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18082.1"
                     /translation="MAAAAGFAAAIFAPDAARACACGCGVFDVATSSMLPQGPGGMAY
                     LELDYQNQYRNWRGNSPSAAVNNTDKEIRTLFVTSGLQYFFNRDWGFQVEVPYDFRTF
                     KTVSNDPNAPAGSIAAPSWSDIGDIRVKGIYTGFSEDLSTGLEFGLKLPTGSYTHEDA
                     FGDVDRDTQLGTGSTDLLLGGFHRQHFTKKGDWTWFAQAELDLPMLIRDDYRPGIELD
                     TAVGIYHEGWKLGRVKITPVAQVIFSERTSDSGEQSANPVASGYQRILLSPGIELDMH
                     PISIYADVEVPVFQHMIGNQLVAPALFKMVFTYHF"
     gene            complement(2279..>2630)
                     /locus_tag="VG754_02400"
     CDS             complement(2279..>2630)
                     /locus_tag="VG754_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18083.1"
                     /translation="IVALLGATGGHWAALQSVAWARMLADNARVAPLSIALEKTFDGK
                     HPCSLCKKIAKGRQGEKKSDAQLEVNKLEFLRSSTTFVIVSPDQFYLMGDRPASPESL
                     TQSPPVPPPRSFPV"
BASE COUNT          575 a          753 c          716 g          586 t
ORIGIN      
        1 ctccgcgagc ttttgcgcga tgaccggcaa aaattcctgc gcgatgctct tgtccaccag
       61 caacgtttca atcgcattgc aaacgccggg acgctggcac ttcgcgttca gcacaatctc
      121 ctcggccatc ttgaaatcgg cgtcgccatc gacataaaca tggcaaacgc ccttgtaatg
      181 tttgatgacc ggcaccttcg agttttccgt cacggcgcgg atcaatccct cgccgccgcg
      241 cggcatgcac aaatccacat attgagtgag cgaaagcaat tccttgatgg cctcgcggtc
      301 gatggtttgc acgacctgga tggcgtgttc gggaaaatga ggcaggcgct gtttcgcggc
      361 agagagcatc acttgcgcga tggcctggtt ggaatgaatc gcttccttgc cgccgcgcaa
      421 aatagtcgcg ttgcccgact tgaagcaaag gctcgcggcg tccgccgtga cgttcggccg
      481 cgattcataa atgatgacca ccacgccgat gggcgtgctg atcttttgca gcttcaatcc
      541 gttcggacgc gtccggctgt cgagaatgcg accgacgggg tcgggcaaag cggccacttc
      601 acgcaaacct tttgccatgg cggcgatgcg tttgtcgtcc aatttaagcc ggtcgagcat
      661 cgcggaggaa agtccgcttt gcgcgcctgc ggccatgtcc ttggcgtttg ccgccttgat
      721 ggtttccgcg ccgtgttcga gcgtgtcggc catggcgagc aggcaggcgt ttttttccgc
      781 cgtggtcagc ctggcgagtt cacgcgaggc cgctttggcc tgtctcgcca attccgtcat
      841 ctgttcccgc aggttcacgg cgtcaaatta agctaaaacc gcaaagttaa aagctgaaat
      901 acgtaatgcc aattcgccct ttgcgcaagg tggtggctcg tgggggaatc aatccatcag
      961 ggtttgacac taaaataggc attcgcagca cgttgtccaa gccggattca gtttaatggg
     1021 gagcacacgc ctccggcgtg tagtgttcgg cgtctcgccg aacacttcgt aatactgatt
     1081 ttctgtccac caaacagtgc gccagaactt aaagtcctcg ccgggacggc tcggacagca
     1141 cgcgagacgc gcgcgctccc ccttttcatg attcccgcta aggtgattac tttaaaaatg
     1201 gtaggtgaac accattttaa acagcgcggg cgcgaccaat tgattgccga tcatgtgctg
     1261 aaaaaccggc acttccacgt ccgcataaat gctgatcgga tgcatgtcca attcgattcc
     1321 cggcgagagc aagatgcgtt ggtaacctga cgcaacagga ttggctgatt gctcgccgct
     1381 gtcactcgtc cgttccgaaa aaatcacctg cgccaccggc gtgatcttca cccggcccag
     1441 cttccagccc tcgtgataaa ttccgaccgc cgtgtccagt tcgatgcccg gacgataatc
     1501 atcgcggatc aacatcggca gatccaattc cgcctgcgca aaccacgtcc agtctccctt
     1561 ttttgtgaaa tgctggcgat gaaatccgcc caggagcaag tcggtgctgc cggtgccgag
     1621 ttgagtgtcg cggtcaacgt ccccgaacgc atcttcgtgc gtgtaactgc ccgtcggcag
     1681 cttcagccca aattccagcc cggtggacaa gtcctcggaa aatcccgtgt agattccctt
     1741 cacgcgaatg tcgcctatgt cgctccaact cggcgcggca attgagccag caggcgcgtt
     1801 gggatcgttg ctgaccgtct tgaaagtccg gaagtcataa ggcacttcaa cctggaaccc
     1861 ccaatcgcga ttgaaaaaat attgcagccc ggaagtcacg aacaaggtgc ggatttcctt
     1921 gtcagtgttg ttcacggcgg cggatgggga attgcctctc cagttcctgt attgattttg
     1981 gtaatccaat tcgagatagg ccattccgcc aggcccttgc gggagcatcg aactggttgc
     2041 cacatcgaaa acgccgcaac cgcaggcgca cgcccgcgcg gcatccggcg caaagatcgc
     2101 cgccgcaaaa ccggctgcag cggccatttt caaagaagtt ctaaaattca tataacaatc
     2161 gagagaaatt atttttttaa gggattttcc gcacacacgg attcatgcgc atgttccgcg
     2221 atattttgaa aagattgtcg ttgcacggaa gcgcaacgca atttggacag ccgaaatatc
     2281 agaccggaaa acttcttggc ggaggaacgg gcggggattg agtcaatgac tccggcgaag
     2341 ccggacggtc gcccatcaaa taaaactggt cgggggaaac gatgacgaaa gttgtggaac
     2401 tacggagaaa ttccagcttg ttgacttcga gttgcgcgtc cgattttttt tcgccttgcc
     2461 gtcccttggc gatttttttg cagagagaac agggatgttt gccatcgaaa gttttttcca
     2521 acgcgatgga aagcggagcg acccgcgcat tgtccgcaag catgcgcgcc caggcgaccg
     2581 attgcagcgc cgcccaatgc ccgcccgtcg cgccaagcag cgcgacaatc
//
LOCUS       DASZMM010000094         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4162408, whole genome shotgun sequence.
ACCESSION   DASZMM010000094 DASZMM010000000
VERSION     DASZMM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4162408"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..484)
                     /locus_tag="VG754_02405"
     CDS             complement(56..484)
                     /locus_tag="VG754_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HWF18084.1"
                     /translation="MAEARVIIDAGPLVAFLVKQEEHHGWVTEQFQKLPAPFLTCDAV
                     LTEAFFLVRKLPNGAARFFSLLNSGLLVSDFSMIAEISALEKLVQRYSNVPMSLTDAC
                     LVRLSKLHPRAVLFTLDGDFQIYRRDGRQPIPLLMPHSSG"
     gene            complement(477..710)
                     /locus_tag="VG754_02410"
     CDS             complement(477..710)
                     /locus_tag="VG754_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWF18085.1"
                     /translation="MTTLICSIPEKLDARLEVLAKQARVPKDKFVRKALERAVRMPRT
                     NSTAFGLVKELCGSLSGPSDLATNPKYLEDLGG"
     gene            complement(1162..2616)
                     /locus_tag="VG754_02415"
     CDS             complement(1162..2616)
                     /locus_tag="VG754_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF18086.1"
                     /translation="MKAPLDSAAFQSSINRRTFLGRAAFGLGGLALANLLDPNLLRAA
                     DTPDPRIHWRGIINPPHFPIRAKRIIHLYMAGGPSHLESFDYKPKLKELDGKPFPESF
                     TKGQQLAQLQNSQLIARAPFCQFKKWGQSGQEISELFPHIGGIADDICIVRSLHTEQI
                     NHDPAHAFMNSGSQIKGRPSMGSWLLYGLGSETQDLPGFVVFTSAGKSGLQPVSARQW
                     SSGFLPTRFEGILFQSKGDAVHYVGNPDGVCQSTQRQVVEEINRLNGMLAEERIDPEI
                     QTRISQYEMAFRMQTSVPELADFSQETQATLDMYGIKHPGDGTFASNCLMARRLVERG
                     TKFVQLYHRAWDHHGNIEKDMPVAAKDVDQASAALVKDLKQRGMLDDTLVIWGGEFGR
                     TPMGQGTGRDHHILAFSVWMAGGGMKGGVTHGSTDELGYRAVEDSQNMHVHDLHATML
                     YLCGIDHRKLTYRFQGRDFRLTDVSGNVHKAIIA"
     gene            complement(2626..>3603)
                     /locus_tag="VG754_02420"
     CDS             complement(2626..>3603)
                     /locus_tag="VG754_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HWF18087.1"
                     /translation="LPQITDTDGHRLTRLDLAKWLVSRDNPLTARAVMNRTWKQFFGT
                     GISAVVDDLGAQGEWPTHPELLDWLATEFMDSGWNTKHMIKLMVMSSTYRQSSNPRPE
                     IHELDPANRLYASQSPRRLEAEFVRDNALFVSGLLNQDIGGPSAFPYQPAGYYANIQF
                     PNRDYIADTDDRQYRRGLYTHWQRTFLHPMLANFDAPSREECTAIRNVSNSPQQALTL
                     LNDPTFVECSRMLAAKILSNAPRNDDKRLDFAYQTVLVRAVKPAERISLEKFLTVQRA
                     YYADHADDAKKLLHEGLASEPKVAGDPELAAWTQVCRVMLGLHETITRY"
BASE COUNT          769 a          968 c         1050 g          816 t
ORIGIN      
        1 agttgtttaa aatctttgtc gggtgagatg cccggatttg tgacattggc actgttcatc
       61 cagacgaatg tggcatgaga agagggatcg gctggcgtcc gtcccgccgg taaatttgaa
      121 agtcgccatc caaagtgaac agcaccgcgc gcggatgcaa tttggacagt ctgaccaaac
      181 aggcgtccgt caatgacatt ggcacattgg aatatcgctg aaccaatttt tccagcgcgg
      241 agatttcggc aatcattgaa aaatcgctga caagcagtcc gctgttcagc agagaaaaaa
      301 atcttgcggc cccgtttggc aatttacgga cgagaaagaa tgcttccgtc aaaacggcat
      361 cgcaggtaag gaacggcgcg gggagttttt gaaattgttc tgtaacccac ccatggtgtt
      421 cctcctgctt taccaaaaac gcaaccagcg ggccggcatc aatgatgacg cgcgcctcag
      481 ccacccaggt cctccaaata ttttggattg gtggcgagat ccgacggccc gctcaaagaa
      541 ccgcatagtt ctttaacgag gccaaaggcc gtgctgttcg tgcgcggcat gcggacggcg
      601 cgttccaacg ctttgcggac aaatttgtcc ttcggcaccc ttgcttgttt cgccagaacc
      661 tccagccggg cgtccagctt ttcaggtatg ctgcaaatta gcgttgtcat gataaaattt
      721 tatcgttttt cagggacaat gcaataccgt gctgcgttgc agggccaaca ggggtgcatc
      781 ttgacactgt tcttaaatcc gttgtgagaa gcgagagaag gctcgccctc atcccagcct
      841 aatggcttcc caagcccgcc acaagtcagt ttccccaaaa gagacgaacg cttcgcgttg
      901 tgtggtcccc ctgggagaag gagatccggc atccgccctt ttatcattgg ccgcaatcgt
      961 ttgcatcatt cccatcgcgg gaatgttccg aacagcggga aatggtgaaa gccctctcct
     1021 tgggggagag ggtttgggtg agggcgagcg tcatccaaac cgtacctgta cattctcaag
     1081 aacagcagca agatgcaccc aaacaaaccc gttttacatc ttcttatctg cgttcatccg
     1141 cgtttatctg cggtcaaact ttcaggcgat gattgccttg tgcacatttc ccgaaacatc
     1201 cgtaaggcgg aagtcgcgtc cttgaaaacg ataggtaagc ttcctgtgat cgatgccgca
     1261 gaggtaaagc atcgtcgcgt gcaaatcgtg gacgtgcatg ttctggctgt cttcgacggc
     1321 gcgatagccg agttcatcgg tgctgccgtg ggtcactccg cccttcatgc cgccgcctgc
     1381 catccagact gagaacgcga ggatgtgatg atcgcgtccg gtgccctggc ccattggcgt
     1441 gcgcccaaat tctccgcccc aaatcacgag cgtgtcatcg agcatcccgc gttgcttgag
     1501 gtctttgacc agcgcggctg aagcctggtc aacgtccttc gccgcgacgg gcatgtcctt
     1561 ctcgatgttg ccgtgatggt cccacgcgcg atgataaagt tgcacgaact tcgtcccgcg
     1621 ctcgaccagc cgccgcgcca tcaaacaatt cgatgcgaat gtgccgtcgc cgggatgttt
     1681 gatgccgtac atgtcgagcg tcgcctgcgt ttcctggctg aaatcggcga gttccggcac
     1741 ggacgtttgc atccgaaacg ccatttcgta ctggctgatg cgcgtctgga tttccgggtc
     1801 gatgcgttct tcggcgagca ttccattgag ccggttgatt tcctcgacga cctgccgttg
     1861 cgtgctctgg caaacgccat ccgggttgcc gacataatgc accgcgtcgc ctttcgattg
     1921 aaacaggatg ccttcaaaac gggtcggcaa aaatccgctc gaccattgcc gcgccgatac
     1981 cggttgcaaa ccagacttgc cagcagaagt gaaaacgaca aatccgggca agtcctgcgt
     2041 ctcggagccc aatccataca acaaccacga acccatgctg ggacggccct tgatctgcga
     2101 accgctgttc atgaacgcgt gcgccgggtc gtgattgatc tgctcggtgt gcagggagcg
     2161 aacgatgcag atgtcgtccg cgatgccgcc gatgtgcgga aaaagctccg agatttcctg
     2221 gccggattgc ccccattttt taaattggca gaaaggcgcg cgggcgatga gttgcgaatt
     2281 ttgaagttgc gcaagttgct ggcccttggt gaacgattcg ggaaatggtt tgccgtccag
     2341 ttccttcagc ttcggcttgt aatcgaaact ctccagatgc gacgggccgc cagccatgta
     2401 aagatggatg atgcgttttg cgcgaatggg aaaatgcggc ggattgatga tgcctcgcca
     2461 atgaatcctt ggatcggggg tatcggcggc gcgaagcaaa ttggggtcga gcaaattcgc
     2521 gagcgcaaga ccgccaaggc cgaatgcggc gcggccaaga aacgtgcggc gattgatgct
     2581 cgattggaac gccgcggaat caagaggtgc tttcataaaa aattttcagt agcgcgtgat
     2641 ggtttcgtgc aggccgagca tcacgcggca aacttgcgtc catgcggcaa gttctggatc
     2701 gcccgcgact ttcggttccg acgcgagacc ttcgtgcaga agttttttgg cgtcgtccgc
     2761 atgatcggca taataggcgc gttgcacggt caaaaatttt tcgagcgaga tgcgttccgc
     2821 cggcttgacc gcccgcacga ggacggtttg gtatgcgaaa tcgagtcgct tgtcgtcatt
     2881 gcgcggggcg ttagaaagaa tttttgcggc gagcattcgc gaacattcga cgaacgtcgg
     2941 gtcgttcagc aacgtgagcg cttgttgcgg ggagttggaa acattgcgga tggcggtgca
     3001 ttcctcgcgc gagggcgcgt cgaaatttgc cagcatcgga tgcaaaaatg tccgctgcca
     3061 atgcgtgtaa aggccgcggc gatattggcg gtcgtcggta tcggcgatgt aatcgcggtt
     3121 tggaaattgg atgttcgcgt agtatccggc gggttgatac gggaacgcgc tcgggccgcc
     3181 gatgtcctgg ttcaacagcc cggaaacgaa cagcgcgttg tcgcgaacga attcggcttc
     3241 cagccgtcgt ggggattgcg aggcatacaa acgattggcc gggtcaagtt cgtggatttc
     3301 cggtcgcgga ttggaggatt gccggtaggt cgatgacatg accatgagct tgatcatgtg
     3361 cttggtgttc caaccgctgt ccatgaattc ggttgcgagc cagtcgagca attcgggatg
     3421 cgtcggccat tcgccttgcg cgcccagatc atccacgacg gcggaaatgc cggtgccgaa
     3481 aaattgtttc cacgtccggt tcatcacggc gcgcgcggtg agcggattgt cgcgcgaaac
     3541 gagccacttc gccaaatcaa gccgcgtcaa acgatgccca tcggtgtccg taatctgcgg
     3601 cag
//
LOCUS       DASZMM010000095         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1678481, whole genome shotgun sequence.
ACCESSION   DASZMM010000095 DASZMM010000000
VERSION     DASZMM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678481"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VG754_02425"
     CDS             <1..411
                     /locus_tag="VG754_02425"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HWF18088.1"
                     /translation="MTVGFDKLASYNFEVDNAPLTNLTAGADKANDQIPDRVKSLDKK
                     KVSIRGFMLPLKVSNGVVTEFLIMKDQSMCCYGTVPKITEWVSVKTPEHGVKSIMDQP
                     VSIEGTLHVGAMRENGYLIGIYQMDGDKLIAPEN"
     gene            complement(426..932)
                     /locus_tag="VG754_02430"
     CDS             complement(426..932)
                     /locus_tag="VG754_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18089.1"
                     /translation="MLRILIVARCCGSPSRAPGDPGLKLAFTASSPYRRAMDIQIAVL
                     CDAATENGGKLNLLGAFDAIRAPQLPVVHPQCSIALRMTFTRVEEGAHKLRFSFVDED
                     GRSIMPAIDLPFEVRLPDESLFVTTNFIVSIQQLRFEMEGMYSIDVAIDGRREAAIPL
                     VVKLAAQV"
     gene            1072..2478
                     /locus_tag="VG754_02435"
     CDS             1072..2478
                     /locus_tag="VG754_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF18090.1"
                     /translation="MNTEKIDTNQKALQINLDATRYGTFAEIGAGQEVARWFFHVGGA
                     AGTVAKTISAYDMGVSDAIYGSSDRYVSRKRLQSMLDYEFNLILERLNKTRGDRCAFF
                     AFADTVATHSFTRHEEGHGWLGIRFQTECKGEPSEIIIHVRLLDRENVREQEALGIIG
                     VNLIYGAFYHHKEPLAMIGTLMDGLTKDRIEVDMIKLSGPAFTGVDNRLMSLQLVQQG
                     LTDAAMFTADGEVVQPSELLYKKPVLVERGSFRPVTNTTLDMLDRASEQFLTESGMDN
                     DQPVVLMEMTLRNLLSDGGVDHHDFLQRVDVLSALGKIVLVSNYARYYKLVSYLSRYT
                     QKNIGLVLGIPSLKEIFDEKFYTDLEGGLLESLGRLFKNSVKLYVYPWRDPVTGKITT
                     AENLRVAPHLQHLYAHLIENHSVESIQKHNIEYLPIFSRDVLARIQTYDPSWETMVPP
                     TVVEIIKRDQLFGFRELK"
     gene            3075..3308
                     /locus_tag="VG754_02440"
     CDS             3075..3308
                     /locus_tag="VG754_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18091.1"
                     /translation="MAADFKVNEKFYLSPGYQEAEARRDFIDKFLMALGWGVNRDTQK
                     NPYEQKVKVERKERGVSQRRADYDFISRQIFGR"
     gene            3604..>4176
                     /locus_tag="VG754_02445"
     CDS             3604..>4176
                     /locus_tag="VG754_02445"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWF18092.1"
                     /translation="MYFGVDYYPEHWVYPYAGTPEKPEGQWEHDVELMVAAGMNVVRM
                     GEFSWGISEPEEGKYDFEWLRRAMDLMQKAGIQVVLGTPTAAPPIWLAKKHPQILPVD
                     SEGRVVNEGTRHAACLNSNVYWDYSKRIVTAMANALGKHPQLIAWQIDNGLGAHQTEA
                     SFNDETRRDWHSWLQAKYETIQRFNELCGTR"
BASE COUNT          992 a         1145 c         1123 g          916 t
ORIGIN      
        1 atgaccgtcg gcttcgacaa actggcttcc tataatttcg aagtggacaa tgcgccgctg
       61 acaaatttga ccgccggcgc ggacaaggcc aatgaccaga ttcccgacag ggtcaagtcg
      121 ctcgacaaga aaaaagtttc catccgcggt ttcatgctgc cgctcaaggt gtccaacggc
      181 gtcgtgacgg aatttctgat catgaaagac caatccatgt gttgctatgg caccgtgccg
      241 aagatcaccg aatgggtcag cgtcaaaact ccggagcacg gcgtgaagtc catcatggac
      301 cagccggtca gcattgaagg aacgttgcat gtcggcgcga tgcgcgagaa cggctacctc
      361 atcggcatct accagatgga cggcgacaag ttgattgcgc cggagaattg accagaaatt
      421 tcgggttaaa cttgcgctgc caatttcacc acgagcggaa tcgcagcttc acggcgtccg
      481 tcaatcgcaa cgtcgattga atacatgcct tccatctcga agcgcaattg ctggatgctc
      541 acgatgaaat tggtggtgac aaaaagcgat tcgtccggca gccgcacttc gaacggcaga
      601 tcaattgccg gcatgatgga gcggccgtct tcatcaacaa aactgaaacg cagtttgtgc
      661 gcgccttcct cgacccgcgt gaaagtcatt ctcagcgcga tggaacattg cggatggacg
      721 acgggcaatt gcggcgcacg gatggcatca aaagcaccca gcagattcag cttgccgcca
      781 ttttccgtcg cggcgtcgca aagcaccgca atttgaatat ccattgcgcg acgataggga
      841 gatgacgcgg tgaatgcgag ctttaaaccc ggatcacccg gagcgcggct cggtgagccg
      901 cagcaacgcg cgacaatcag aatgcgcaac actacttcat tgcacccacc cttcccatat
      961 tgctgcggct catcgagccg cgctcctgtt ttaaaaacgg ggcttgcggg aagtgacaaa
     1021 tgtctttttg atgtatgtta gtttatcgct gctgggcgat ttgatgaagt catgaatacc
     1081 gaaaaaattg atacgaacca aaaagcgctt cagatcaacc tggatgcgac gcgttacggc
     1141 acgttcgccg agatcggcgc gggccaggaa gtggcgcgat ggttctttca tgtgggcggc
     1201 gcggccggca cggtcgccaa gacgatttct gcatacgaca tgggcgtgag cgatgcgatt
     1261 tacggatcaa gcgaccgtta tgtgagccgg aagcgtcttc aatccatgct cgattacgag
     1321 ttcaacctca tcctcgagcg gttgaacaag acgcgaggag accgttgcgc tttttttgct
     1381 ttcgcggaca ccgttgccac acacagtttc acgcggcatg aggaaggcca cggctggctg
     1441 ggcatccgtt tccaaaccga atgcaaaggg gagccgtcgg aaatcatcat ccacgtgcgg
     1501 ctgcttgacc gcgaaaatgt gcgcgaacag gaggcgcttg gcatcatcgg cgtcaacctg
     1561 atttacggcg cgttttatca tcacaaggag ccgttggcga tgatcgggac gctcatggac
     1621 ggcctcacca aggaccgcat cgaagtggac atgatcaagt tgtcagggcc ggcatttacc
     1681 ggtgtggaca accgcctgat gagcctgcaa ctcgtgcagc aaggcttgac cgacgccgcg
     1741 atgttcaccg cggatggcga ggtggtgcag ccaagcgagc ttctgtataa gaagccggtg
     1801 cttgtcgagc gcggcagctt tcgtcccgtc accaacacca cgctcgacat gctcgaccgc
     1861 gcgtcggaac aatttttgac cgagtcgggc atggacaacg atcagccggt ggtgctcatg
     1921 gaaatgacgc ttcgcaacct gctgtccgat ggcggagtgg accatcacga ttttttgcaa
     1981 cgcgtggatg tgctgagcgc gctgggcaaa atcgtattgg tctcaaacta tgcgcgttat
     2041 tacaaattgg tttcatatct ttcgcgttac acccagaaga acatcgggct ggtgctgggc
     2101 atcccgagtt tgaaggaaat ttttgacgag aaattttaca cagatcttga aggcggtttg
     2161 ctggaatcgc tgggcaggtt gttcaagaat tccgtgaaac tctatgttta tccctggcgc
     2221 gacccggtga ccggcaagat aacgacggcg gagaaccttc gcgttgcgcc gcacttgcaa
     2281 catctgtacg cccatctcat cgagaaccat tccgtggaat ccatccagaa gcacaacatc
     2341 gaatacctgc cgattttttc gcgcgatgtc ctggcgcgca tccagaccta cgatccatcc
     2401 tgggaaacca tggtgccccc tacggtcgtc gagatcatca agcgcgacca attattcggc
     2461 ttccgcgaac taaaatgacc cctgggaggg acggtgcgct gcgccgtccg ctctggcccc
     2521 gcgtccctca ccatcaacca tcaactctca accatttggt ggagcgagcg tacccgcgag
     2581 ccgtttcaat aattcgtagg tgccgacgtg aggaggctct gatttttttg acaaccgcag
     2641 atgaacgccg attgacgcag attcgaggcc atgcaacttt gctcccttcg cggacttcgc
     2701 gcgacccatc cagcgccgta ggcgcggcat ctttgtagaa ccaatcgcat cagaaataat
     2761 cagccccgga gcggccctga cagggccgaa ctggtccgtt ggcattcatc cttgggttcg
     2821 cacccacggc tattcgtgtt tttgccactt cgtggcaaga acagtcaaga tgcgcaccca
     2881 ggaccccgcg agccgttgcc caaactttaa attttaaatc attaaatctt gaacttgtca
     2941 ccccgttgca gccgacgtga ggaggctctg atcaagattg ccggttgcgc ccttgctcag
     3001 tcttccagtt atttgatagt gtttgctccc agatgccttc caatttcgat caagcgtttg
     3061 aacgggtaaa gcagttggcc gcagatttta aggtcaatga gaaattttat ctttcgccgg
     3121 gataccagga agcggaggcg cggcgggatt tcattgataa atttttgatg gcgcttggct
     3181 ggggcgtaaa ccgcgatacg caaaagaatc cgtacgagca gaaagtgaag gtggagcgca
     3241 aggagcgtgg ggtttcgcaa cgccgcgcgg attatgactt tatctcgcgc caaattttcg
     3301 gaaggtaaaa ttttatattg aggcaaaaaa atcgtttggg gacattgccg cgccgacaat
     3361 tattttcatg gcgaccatca aaaaccgcac tttgtcgcag cttgtctcac atcaactcag
     3421 atcaaccggg ataaaccggg ctatggaaaa caaatttttt cgcacgacca tcaggccgcc
     3481 gccctgaaga taatggcgcg tgtgccgagt cgtgcccgca acagcctcat ccccatttta
     3541 agattgctta ttttccctgc tttgttacaa tttcatgaga aacaatccaa cccacccaaa
     3601 attatgtatt ttggcgtaga ttattatccc gaacattggg tctatcctta cgccggaacc
     3661 cccgaaaagc ccgaaggcca atgggaacat gacgtggaat tgatggttgc cgccgggatg
     3721 aacgtggttc ggatgggcga gtttagctgg ggcatcagcg aacccgagga gggcaaatac
     3781 gatttcgaat ggctccggcg cgccatggac ctgatgcaaa aggcaggaat ccaagtcgtt
     3841 ctcggcacgc ccaccgccgc tccacccatc tggctcgcca agaaacatcc ccaaatcctg
     3901 ccggttgatt ctgaaggccg cgttgtcaac gaaggcaccc gccatgcggc ctgtctcaac
     3961 agcaatgttt attgggatta ttccaagcgc atcgtcaccg caatggccaa cgcgcttggc
     4021 aaacatccac aactcatcgc ctggcagatt gacaatggcc ttggcgcgca ccagaccgaa
     4081 gcttctttca acgatgaaac ccgccgcgac tggcattcgt ggctccaggc caaatacgaa
     4141 accatccagc gtttcaacga gctttgcggc acgcgg
//
LOCUS       DASZMM010000096         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3338350, whole genome shotgun sequence.
ACCESSION   DASZMM010000096 DASZMM010000000
VERSION     DASZMM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338350"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..619
                     /locus_tag="VG754_02450"
     CDS             386..619
                     /locus_tag="VG754_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18093.1"
                     /translation="MSYKTLEVELENGRVHPSGAETLPPKAHALLTILSANESAEALN
                     GSLADRVAGLSGIGHGKYSDLSTNKSHLNDLGR"
     gene            690..1112
                     /locus_tag="VG754_02455"
     CDS             690..1112
                     /locus_tag="VG754_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HWF18094.1"
                     /translation="MSGFLTNSPGGLICEAVLAEVCHLVAKDGISRGKVLEFVEQGGL
                     MPVFLGGEFRVLRELLNRYADTPMDFADACVVRLAEIHADSAVCTTDGDFQFFRKNGR
                     EPITFTGSVLNLYCPLNFARSSRPRAVRIVVAQTHPFS"
     gene            1125..>2000
                     /locus_tag="VG754_02460"
     CDS             1125..>2000
                     /locus_tag="VG754_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HWF18095.1"
                     /translation="MKSILSEASRVPFAAQVEEIVMSAPIHDVHTHLYEAAFGGLLLW
                     GIDDLLVYHYLVAEAFRYLTIPYEKFWSLSKTEQADLIWRELFLEHSPISEACRGVLT
                     TLQAFGLDVKKRDLAAVRKWFAAQTVQGHITQCMELAKLKSICTTNSPFDDMERAVWE
                     KGFARDERFVTALRIDPLVLSWTETAPKLAQWGYRVSADPASGQTFSEVRRFLADWSK
                     RIKPRYVMVSLPPDFQFPAKTDAAAIIEHAVLPHCREFGLPFALMPGVIRAVNPQLKL
                     AGDGEGLTDLTMLRNL"
BASE COUNT          420 a          558 c          557 g          465 t
ORIGIN      
        1 aaaaggagcg cgccaaagtt cagggatgaa agcccggcat gtgacagcct gggctcgatc
       61 gagcatcgcg agcgctggcc caggatttgg ttgccagaaa tgtgtaaagc ctgaagggcc
      121 gtaacaaact ggatgttttc ccacgattat tgttccgccc ttttcagggc tgaaaatccg
      181 ttgtaacgca ttacctgggg ctgtgcgcgt cgcgcttcac tccaggctat aacattgcgg
      241 gctttcagcc ccgcatttga tttggcaccg gtttctgggg cgttgccgca tctggcatga
      301 aacgcgccca tggcacttat cagagcaaaa aaaatgtgac aaacaccgac cttgccgcaa
      361 taaaaaaact tggtaagctt tttccatgag ctacaaaacg ctggaagtgg aattggaaaa
      421 cgggcgcgtc cacccgtccg gcgcggaaac gctgccgcca aaagcccacg cgctgttgac
      481 cattctgtcc gcgaacgaaa gtgctgaagc tctcaatggc agtctcgctg accgcgttgc
      541 cggcctttct ggcattggac acggcaaata ttccgacctt tcgaccaaca aatctcacct
      601 gaatgacctt ggccgatgaa gccggtcatt tttgacaccg ggccattggt ggcgtggttt
      661 tgcccgcgag ataaacatca tgcttgggcg tgtcaggctt tctcacaaat tcccccggcg
      721 gattgatttg cgaagcggtt ttggctgaag tctgccacct ggttgcaaag gacggaatct
      781 cgcgcggcaa agttttggaa tttgtggagc aaggcggact gatgccggtt tttctcggcg
      841 gtgaatttcg agtgttgcgc gaactgctca atcgttacgc cgacacgccc atggattttg
      901 ccgatgcgtg cgtcgttcgc ctggcggaaa ttcatgcgga cagcgcggtt tgcacaaccg
      961 atggcgattt ccaatttttc cgaaagaatg gccgtgagcc gattacgttt actggctccg
     1021 ttttgaacct ttactgcccg ttaaacttcg ctcgctcttc gcgtccaagg gccgtcagaa
     1081 ttgtggtggc gcaaacgcac cctttttcct aatctaacac tccgatgaaa tctatcctgt
     1141 ccgaggccag ccgcgtcccc tttgcggcgc aagtcgagga gatcgtgatg tccgcgccga
     1201 tccacgatgt ccacacgcat ctgtacgagg cggctttcgg cggcttgctg ctttggggca
     1261 ttgatgattt gctggtttat cattatctgg tggccgaggc gtttcgttac ctgacgattc
     1321 cctacgaaaa attctggtcg ctctcgaaga ccgaacaggc cgacctcatc tggcgcgaac
     1381 ttttcctcga acattcgccg atctccgagg cgtgccgcgg cgtgttgacg accttgcagg
     1441 cgttcggctt ggatgtgaaa aagcgcgacc tcgccgcagt ccgcaaatgg ttcgccgcgc
     1501 aaactgttca aggccacatc acgcaatgca tggaattggc gaagctcaaa agcatttgca
     1561 cgacgaattc tccgttcgac gacatggaac gcgccgtctg ggaaaaagga tttgcgcgcg
     1621 atgaacgttt cgtgacggca ttgcggattg acccgctcgt gctctcctgg acggaaaccg
     1681 cgcccaagct cgcgcaatgg ggttatcgcg tcagcgcaga cccggcgtca ggccagacct
     1741 tcagcgaagt gcggcgattt ttggccgatt ggtccaaacg catcaagccc cgttacgtga
     1801 tggtgtcatt gccgccggat tttcaatttc ccgcaaagac ggatgccgct gcaatcatcg
     1861 agcacgccgt cctgccgcat tgccgcgagt tcggattgcc attcgcgctc atgccgggcg
     1921 tgattcgcgc ggtaaatccg caactcaaac tggcgggtga cggcgaaggc ttgacggatt
     1981 tgaccatgct gcgaaacctc
//
LOCUS       DASZMM010000097         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1127349, whole genome shotgun sequence.
ACCESSION   DASZMM010000097 DASZMM010000000
VERSION     DASZMM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127349"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VG754_02465"
     CDS             complement(<1..749)
                     /locus_tag="VG754_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF18096.1"
                     /translation="MKLTIPIATGLFALLLNCEILSHTARAEPAHGWLNWRGPQQNGT
                     SLETGLPDSVDPQHPLWVADFPGQSTAVIANGHLYIVGYLGEGENLQEGIICYDAETG
                     KELWRQLFSDYLSDTIYLRYGTSSPAVDPETGNVYLQGTQGILAAFTPDGQMLWQHSL
                     MEEYGRLTFPNSRTASPVIEGDLVITRGITANWGGNGPAADRFYAFDKKTGALVWSST
                     PGAQPKDNSFSHPVIGWLDGKRVLYSATGDGG"
     gene            852..1367
                     /locus_tag="VG754_02470"
     CDS             852..1367
                     /locus_tag="VG754_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWF18097.1"
                     /translation="MATEAHSRKKPAAHSDPELPVDAPGRRRLPALLRRCWFNLNQTF
                     RRRIAHTGVTPDQFTVMRTLLEGDAKGMTQRELTDLMSSDPNTIAALLERMEKSGLVE
                     RKQHELDRRAYRISLKPIGRKKYDEVREIALTLQMEVLSVLPQEKQEEFLKHLELVSD
                     ACRLAAEKIGK"
     gene            1574..2209
                     /locus_tag="VG754_02475"
     CDS             1574..2209
                     /locus_tag="VG754_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HWF18098.1"
                     /translation="MGEPLWLYRLRTVTGSELLAGLMGFGAGWMQARIVPKLNLSKVG
                     AQLLVPFLLVFGIALPYLKPIFRPLHTSNLREIWQDNVCLQSSFSTCGPASAATVLRN
                     LGGNVSERELAEESFSCSSGTENWYLARALRRRGFMTTFVRDERFRSLPAIAGVRLKQ
                     ADNSGHFIALLEWQGDRLVTGDPMVGRSTNTLAELREKYDFTGFAMLVRRK"
     gene            complement(2223..2483)
                     /locus_tag="VG754_02480"
     CDS             complement(2223..2483)
                     /locus_tag="VG754_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HWF18099.1"
                     /translation="MSALTVNLPDNLHEKAREIAASKNLSMDALVSIALAQSLSRLVA
                     DPHLEERAARATAKGLDEFLAQVPNAKPQELDHISKDYKAKE"
     gene            complement(2514..2675)
                     /locus_tag="VG754_02485"
     CDS             complement(2514..2675)
                     /locus_tag="VG754_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWF18100.1"
                     /translation="MDPNQQLYIHCKSGVRSMRALNFLREQGFKYVKSVKGGINAYAD
                     EIDHSVPKY"
BASE COUNT          595 a          687 c          760 g          637 t
ORIGIN      
        1 ccgccatcgc cggtcgcgga ataaagaacg cgcttgccgt ccagccagcc gatgaccgga
       61 tgcgaaaacg aattgtcctt gggctgcgcg ccgggcgtgg acgaccagac caatgcgccg
      121 gtctttttat cgaatgcata aaaacggtcc gcagcggggc cattgccgcc ccagttggcc
      181 gtgatgccgc gcgtgatgac gagatcgcct tcaatcaccg gcgaggcggt gcggctgttc
      241 gggaaggtaa gccgcccgta ttcttccatc aacgaatgtt gccagagcat ttggccgtcg
      301 ggcgtaaagg ccgcaagaat gccctgcgtt ccttgcagat aaacattgcc ggtttccgga
      361 tccaccgccg gactggacgt tccgtaacgc agataaatcg tgtcactcaa ataatcgctg
      421 aacaattgac gccaaagttc cttgccggtc tcggcgtcgt agcaaatgat gccttcctgc
      481 aggttttcgc cttcgccgag ataaccaaca atgtaaagat ggccgttcgc gatgactgcg
      541 gtggattggc cgggaaaatc ggcgacccaa agcggatgct gcgggtcaac ggaatcgggc
      601 aggccggttt cgagcgaggt gccgttttgt tgcgggccgc gccaattgag ccagccgtgc
      661 gccggttcgg cgcgcgcggt gtgtgaaagg atttcgcagt tgaggagaag cgcaaacaaa
      721 ccagtcgcga tcggaatcgt cagtttcata aaacagccag caagtgccta atttgcttcc
      781 aagagtcttc ccattattct acaaattccg gggcgacgca atcaaataat acttacacgt
      841 ataatagaat tatggcgaca gaagcgcatt ccaggaaaaa accggcagcc cattccgacc
      901 cggaactgcc ggtggacgcg ccgggacgcc gccgtttgcc ggccttgttg cgccgttgct
      961 ggttcaattt gaaccagacg ttccgccgcc gcatcgcgca cacgggagtg acgcccgacc
     1021 agttcaccgt gatgcggacc ctcctcgaag gcgacgccaa gggcatgacc cagcgcgaac
     1081 tgaccgattt gatgagcagc gatcccaaca caatcgcggc tttgctggag cggatggaaa
     1141 aaagcggcct cgtcgaacgc aaacaacatg aactggatcg gcgcgcctat cgcatcagcc
     1201 tcaaaccgat tggccgcaag aaatacgacg aagtccgcga gattgcgctg acattgcaga
     1261 tggaggtttt gtccgtgctg ccgcaggaga agcaggagga atttttgaag catctggaat
     1321 tggtgtccga tgcctgccgg ttggctgcgg agaagattgg aaaatagcgc gtaaatagtt
     1381 tgcgagatgg cgttggggtt tactgatgaa ttggtttttg attccaactt tattgctcgg
     1441 cattggaaca ttcgttgttg gaaaacggtt ggcaatgcag atgtcttctc gatggctgaa
     1501 ctggctgtgc tgttgtgtct ttcttgtgtt ggtgcttcct ggattcttat atgcaattta
     1561 ttactcgaaa ttgttgggtg aaccgctatg gctgtaccgg ctgcgaacgg tgaccggctc
     1621 ggaattactc gcagggctga tgggatttgg cgcgggatgg atgcaggcgc gaatcgttcc
     1681 caaactgaat ttgagcaaag tcggcgccca attgttagtt ccattcctgc tggttttcgg
     1741 gattgcgctt ccatacctga aacccatttt ccgtccattg cacacgtcga atctaaggga
     1801 gatttggcag gacaatgttt gcctgcaatc ttcattctcg acctgtggcc cggcatcagc
     1861 ggcaaccgtt cttcggaatt tgggtggaaa tgtctccgag cgggaattgg ctgaagaatc
     1921 cttctcatgc tcttccggga ctgaaaattg gtacttggcg cgtgcattgc gtcggcgcgg
     1981 gttcatgacc actttcgtgc gagatgagcg tttccgttcg cttccggcga ttgcaggcgt
     2041 gcgattaaag caagcggata actcaggtca tttcattgca ttgctggaat ggcagggtga
     2101 tcgcctggtg accggcgatc cgatggtcgg acgatccaca aatactttgg cggagttgcg
     2161 agaaaaatat gacttcacag gattcgcgat gctggtcagg cgcaagtgaa cgacgccatc
     2221 aactattcct tcgctttata atccttggag atatggtcca attcttgtgg tttcgcgttt
     2281 ggcacttggg ctaaaaattc atccaggcct ttggcagttg cgcgggcggc gcgttcttcc
     2341 agatgaggat cggcaaccag acgcgaaagg ctttgggcaa gggcgatgct cacaagcgca
     2401 tccatggaga gattcttcga ggccgcaatc tctcgcgctt tttcgtgcag attatccggc
     2461 aaattcacgg tcaatgcgct catattgaaa gaattttcaa aattcacccg gactcaatac
     2521 ttcggcacac tatgatcaat ctcgtccgcg taagcattga tgccgccttt gacgcttttc
     2581 acatacttaa atccctgttc acgcaagaaa tttagcgccc tcatcgagcg gacaccgctt
     2641 ttgcaatgga tgtaaagctg ctggttcggg tccagctcg
//
LOCUS       DASZMM010000098         5708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_28689, whole genome shotgun sequence.
ACCESSION   DASZMM010000098 DASZMM010000000
VERSION     DASZMM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28689"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VG754_02490"
     CDS             complement(<1..1068)
                     /locus_tag="VG754_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18101.1"
                     /translation="MLRRCAVALILVAVAFAIRLALGPWLGEELPFMLFSAAALLAAA
                     YGGAVPGFLALVLGLFLGDVFQESSKNMLIVSESLNHLRTIRYLFNISVGITLIEVLR
                     RNYLRMKLEVAKRKASEAQLMEAKAELGRHAETLEWMVAERTSKLEASLKSIEGMVYH
                     IAHNLRAPLRAMSGFANLLQEEYGSKLDDQGKEYTRRIAAASRQMDELIADLLAFGRL
                     GYVEMNPEKTDLKRLIQMSLQHMAEKIRAKQAQVELIGAFPEVWVSRSGLGQALCHLL
                     DNSLKFVAPGVKPRIQFQSEERGRFLRLKIQDNGIGVESAYHERIFQPFERLQGDAYE
                     GTGIGLAIVQEAVHRMGGKAGV"
     gene            1643..2317
                     /locus_tag="VG754_02495"
     CDS             1643..2317
                     /locus_tag="VG754_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18102.1"
                     /translation="MFGQSKAIEKQLARANGRLKAAATALAPKHKGGEIEEFHAAHED
                     VLRLERELAASRNEPYAIPCDFPVKWDVGAPLPFVFCSDNRTLLTFFIGDRDSGWDGT
                     RVKVVNPASIEVVSLCLVSFKGCASAKLGHPNDEVQSGHPLSGRGLKGYTAQIVKNSP
                     WLKEVAKTNSIHRCDNPESWNLLNHYVFWFHDSTFECLAKSYEVEVSSEAMPELLKRV
                     QEKLLA"
     gene            complement(2543..2617)
                     /locus_tag="VG754_02500"
     tRNA            complement(2543..2617)
                     /locus_tag="VG754_02500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Val,seq:cac)
     gene            complement(2791..>5708)
                     /locus_tag="VG754_02505"
     CDS             complement(2791..>5708)
                     /locus_tag="VG754_02505"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HWF18103.1"
                     /translation="PSLMGSRNYQVLEIVPGSAPTASIPTNGGFEIGSGTTASNWVVD
                     LAAGGPVYAVRTNNNPHSGSSDFEVHLASTGAGPVVEFNQSGVPVTGGTIYPFTFFAS
                     ALTGSQGYNAQWRILWNAGGDTGYQGFSPGNNTYAFVSNNVTAPASATSAIIFFHFAG
                     AAIPSQSATIDLDDVALGTGSSPGTPGVTNILQVTRQAMGNVSFPTTSGIRYQPESAT
                     NLSGIWTSNYPVVIGDGGTDSVQFALTSSSMFFRVATPPVVILPPTNLQQIASGFTNG
                     IGLTWTASPTPGVTGYRILYSLSGGGPTNSMDVGNVNSAILSGLTAGQTYSIAVITLT
                     SGGQSVGVATLTAQPDTTVGIVPLFDAGTALEPDTFSNTPTALITWIADRPRARHARE
                     SQFMLYDTYLIFYWEQRMTDIQIIDTYGKGGATSNLTFNISTLNALDQPNIRFFFQGQ
                     TTVAQYSDNLFADQVDSSGTNWTSTISQNLTYNRPLQVGDRVEVEFSPFMLTVTNGQL
                     NYYGGAILYIVGQGIVPWQEGVTNDAPSVNAALDSTPIPTNGWLAGANTMPYQYSAEP
                     NHVFNQLSPDASPPTGESFLLGRRLHETDFGDGSHSEAGNPIYTQQVGKLGPKFSNRS
                     CVACHVNNGRALPPSIGAPMLQSVVRVGSDASGTPDPVLGSVLQPQITNGTPEDTVTI
                     SSYTTINGQYGDGTTYTLQKPNYTFGSYTPAFYSVRLAPQLVGMGLLEAVSEITVTGL
                     ANADNSGTNGIAGRIQSVLDPQTGQQRLGRFGYKGGKARISHQIAAALNTDMGVTTSI
                     YPTLDGDTTNGPVELNDSDLTNWTRYISSLGVNARRSLTDAQALQGEQLFASANCVQC
                     HTPTLQTSPYHPIAELRNQTIHPYTDLLLHDMGPGLADNMGEGKATGSQWRTSPLWSI
                     GLTAGISGGEAYLHDGRARSLEEAILWHDGEGAAAKEAFRTMSASDRAALIAFLKSL"
BASE COUNT         1304 a         1543 c         1605 g         1256 t
ORIGIN      
        1 aacccctgct ttcccaccca tccgatgcac cgcctcctgg acaatggcca atccaattcc
       61 cgttccctcg taagcgtctc cttgcagtct ttcaaatggc tggaaaatgc gttcgtgata
      121 cgccgattcc accccgatgc cgttatcctg aattttcaat cgcagaaaac gcccccgttc
      181 ttctgattga aattgaatgc gcggtttcac tcccggggcc acaaatttca aggaattatc
      241 cagcaaatgg caaagcgcct ggcccaatcc gcttcgagaa acccacactt ccggaaaggc
      301 cccgattaat tcgacttggg cttgcttcgc ccggatcttt tcagccatgt gttggagcga
      361 catctgaatc agtcgtttta agtcagtttt ttcgggattc atttcaacat agccaaggcg
      421 tccgaaggcc agcaaatccg cgatcagttc atccatctgg cgggacgctg ccgcaatgcg
      481 gcgggtatat tcttttcctt ggtcgtccag cttgctgcca tattcctcct gcaataggtt
      541 ggcgaacccg ctcatcgccc gcagcggcgc gcgcaggttg tgagcgatat gatagaccat
      601 gccttcgatt gatttaaggc tggcttccag tttcgaagtc cgttccgcga ccatccattc
      661 caaggtttcc gcatgacggc ccaactcggc cttggcctcc atcaactggg cttccgaagc
      721 ttttcgcttc gccacctcca gtttcatccg caaataattt cgacgcagga cttcgataag
      781 cgtgatcccg acggaaatat tgaacagata ccggatggtg cggagatggt tcagtgattc
      841 cgaaacaatc agcatgttct ttgaggattc ctgaaaaaca tctccaagaa aaagccccag
      901 gaccaaggcg agaaatcctg gcaccgcccc tccatacgcg gcagcgagca acgcggctgc
      961 ggaaaaaagc atgaaaggaa gttcctcacc aagccaaggc cctaaagcga gccggattgc
     1021 aaaagcaacc gcaaccaaaa tcaacgccac cgcgcaacgc cgcagcattt cagggggggt
     1081 tttgcgaacg gcacgaacat cattcccatt cggtgcccca ttacctttgc cacggacgtt
     1141 agtcgagttc gagggcaaat cctctctttt ggaccagtgg agtggtaaat tcatttgaca
     1201 taaacaattc gactcttagc cgcacctgat gaacccgatg gccagccatg cagcattttt
     1261 caaagcaata aaataacacc gcaacttact tcatctttag atgttttgac agggtcgaaa
     1321 attcaaaatc cgggacgaat ccggattggc ttcaaagatg cggaatggtg cgcgctgcag
     1381 gtttcgaact gtatgaagaa ggctccgttt tgagaatcaa ggactcccgc gaaaaacgtg
     1441 aaagccaagg aggtgggtat tcttccgcgc ttggggacgg gaacctcaca agcgaagcat
     1501 tgtttatgcc ccggctccgg gcgcgaaaat ctcaatgtct ttggcgagaa tcgccttgtg
     1561 tcaccgttcc cgttaaaggc ttgattaatg ccgttggatc aaagtattta tagggtaccc
     1621 gtatttgaaa aaggaataag tcgtgttcgg ccaatctaag gcaattgaaa agcaacttgc
     1681 gcgggcaaat ggtcggctca aggccgcagc aaccgcgttg gcacccaaac ataaaggtgg
     1741 cgagatcgag gaatttcacg ccgcgcacga ggatgtgcta cgcttggaac gagagttggc
     1801 cgcgtcaagg aacgaacctt atgcaatccc atgcgacttc cctgtaaaat gggatgttgg
     1861 agcaccatta cccttcgttt tctgtagcga caatcgaact ttgttgacgt ttttcattgg
     1921 cgaccgggat tctggttggg atgggactcg cgtcaaagtc gtgaatcccg cttcaataga
     1981 agtggtttcc ctctgcttgg tatcgttcaa gggctgcgct tccgccaaac tcggacatcc
     2041 caatgacgag gtacaatccg gtcatcctct gtcgggacgc ggtcttaaag gttacacggc
     2101 acaaattgta aagaattcgc cgtggctgaa agaagtggcc aagaccaaca gtatccatcg
     2161 gtgcgacaac ccggaaagtt ggaatttact caatcactat gtcttctggt ttcacgacag
     2221 cacctttgaa tgtctcgcaa agtcgtacga ggtggaggta agctccgagg ccatgcccga
     2281 actattgaag cgtgtgcaag agaaactcct tgcatgaaag gcgcaaaaca gtcaaatgcg
     2341 tgaaacatag gtgtggcaat tccacaccaa gccgaaaaga tgattcgggg cgaaaattgt
     2401 aaaccgtgca agggcgcagt gctggttgca aatttgacac gaccaggctg agatcggggt
     2461 aagcgttcgc atgggttagc cacgggcttg gtcttgggtt cgcaagcgtc acgtcaatcg
     2521 agcagggcca gaagtgtcaa aatggtgcgc gctgcaggtt tcgaacctgc gacccctacc
     2581 gtgtgaaggt aatgctctac cactgagcta agcgcgcaat ctagactcta agaccttcgg
     2641 aaactgttga ttccacaggt ctttccgcgt tttaacggaa ttcaagggtc agctatttcc
     2701 agttatttgg caaggccaaa aactcggatt ggaaattcag aagcagcttt tggcccgttc
     2761 ggcggaaaca gttgccgcca gcctttatgg tcaaagcgat ttcaagaacg cgatcagggc
     2821 ggcccggtcg gaggcggaca tcgtgcgaaa ggcctctttt gccgccgcgc cttcgccatc
     2881 atgccagagg atggcctcct caaggctgcg ggcccggcca tcgtggagat acgcttcacc
     2941 gccactgata ccggccgtaa ggccaatgct ccagaggggc gacgtgcgcc attgggagcc
     3001 tgtggccttg ccttcaccca tgttgtcggc aaggcccggc cccatgtcgt gcaaaagcaa
     3061 atcagtataa ggatggatgg tctggttgcg aagctcggcg atcggatggt aagggctggt
     3121 ttgcagggtc ggcgtgtggc attgcacgca attggcgctg gcgaacaact gctcgccttg
     3181 caatgcttgc gcgtccgtca ggctgcgacg ggcgttgaca ccgagtgagg aaatgtagcg
     3241 cgtccaatta gtgaggtcgc tgtcgttgag ttcgaccggt ccgtttgtgg tgtcgccgtc
     3301 aagggtcgga taaatcgagg tcgtcacgcc catgtcggtg ttgagagcgg cggcgatttg
     3361 gtggctgatg cgggcctttc cgcctttgta gccgaatcgc ccgagacgtt gctggccggt
     3421 ctgcggatcc agcacagact ggatgcggcc agcgattccg ttcgtgccgg aattatccgc
     3481 gtttgccaag ccggtgacgg taatctcgct gaccgcctcg agcagaccca ttcccacaag
     3541 ttgcggcgcc aggcgcacgg aataaaaggc cggtgtataa cttccaaacg tatagttcgg
     3601 cttttgcaac gtataggttg tgccatcgcc gtattgcccg ttgatggtgg tgtaactgga
     3661 aatggtgacc gtgtcttccg gtgtcccgtt ggtgatttgc ggttgcaaca cggaaccaag
     3721 gaccggatcc ggtgtcccgc tcgcgtcgct gccaaccctg acaactgact gcagcatcgg
     3781 cgcgccaata gaaggtggca gggcgcggcc attattgacg tgacaggcga cgcaactacg
     3841 attgctgaac ttcggaccga gcttgccgac ctgttgggta tagatcgggt tgcccgcctc
     3901 ggaatggctg ccgtcgccaa aatccgtttc gtgcaaccgc cgacccagaa gaaacgactc
     3961 gccggtgggc ggcgacgcgt ccggggacaa ttgattgaat acatggttcg gctcggcgga
     4021 atattggtac ggcatggtgt tggcaccggc gagccagcca ttcgtgggaa tgggtgtgga
     4081 atcaagcgcc gcgttgaccg acggggcgtc atttgtcacg ccttcctgcc acggcacgat
     4141 gccctgcccg acgatgtaga ggattgcgcc accataataa ttaagttgcc cattggtgac
     4201 cgtaagcatg aacgggctga attcaacttc cacgcgatca cccacctgca acggcctgtt
     4261 ataggtcaaa ttctggctga tggttgatgt ccagttggtg ccgctcgaat cgacctggtc
     4321 cgcaaaaagg ttgtcactgt attgcgccac ggtggtttgg ccctggaaga aaaagcggat
     4381 gtttggctgg tccagcgcgt tcagggtcga aatattgaag gtcaagttcg aggtcgcacc
     4441 gccttttcca tacgtgtcaa tgatttggat gtcggtcatg cgttgctccc aataaaaaat
     4501 caagtaagtg tcatagagca taaactggct ctcccgcgca tggcgggcgc gcggacgatc
     4561 tgcgatccaa gtgatgagcg ccgtgggcgt gttggaaaat gtgtccggct ccagtgccgt
     4621 gcccgcatca aacaacggaa caatgccgac ggttgtgtcc ggctgggccg tgagagtggc
     4681 gacgccaacg ctctgtccgc cggaagtgag ggtgatgacc gcaatcgagt aagtctgccc
     4741 ggccgttaaa ccggaaagga tggcggagtt gacgttgcca acatccatcg aattagtcgg
     4801 acctccgcca gacagactgt aaagaatgcg gtagcccgtg acgccgggtg tggggctcgc
     4861 agtccatgtc aggccaatgc cgttggtgaa gcccgatgca atctgttgga ggttcgtcgg
     4921 cggcaggatc acgactggcg gagtcgccac gcgaaaaaac atcgaactgc tcgtcaacgc
     4981 gaactgcact gaatcggtcc cgccatcgcc gatgaccacg ggatagttgg acgtccagat
     5041 gcccgaaaga ttggtggcgg attccggttg ataacggatg ccggaagttg tgggaaagct
     5101 gacgttgccc atcgcctgcc gggtaacttg cagaatgttc gtaacgcccg gagtgccggg
     5161 gctgctgccc gtgcccagcg caacatcatc aaggtcaatc gtcgccgatt ggctcggaat
     5221 cgccgctccc gcaaagtgaa aaaatatgat ggccgaagtc gccgacgccg gagcggtgac
     5281 gttgttgctc acgaaagcat acgtgttgtt cccgggagaa aagccttgat aacccgtgtc
     5341 acctccagcg ttccataaaa ttcgccactg cgcgttataa ccctggctgc ccgtcagcgc
     5401 gctggcaaaa aaagtgaacg gatagattgt ccctcccgtc accggcacac cggattggtt
     5461 gaactcaacc accggtcccg cgcccgtgct cgccagatgc acctcaaaat cagacgagcc
     5521 actgtgcgga ttgttgttcg tgcgcacagc gtaaaccggg ccgccggcgg caagatccac
     5581 cacccagttg ctggccgttg tgccgcttcc gatttcaaac ccgccattcg ttggaatcga
     5641 agccgtcggc gccgagccgg gaactatttc caaaacttga tagttgcgcg accccattag
     5701 cgacggat
//
LOCUS       DASZMM010000099         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1416796, whole genome shotgun sequence.
ACCESSION   DASZMM010000099 DASZMM010000000
VERSION     DASZMM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416796"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VG754_02510"
     CDS             <1..373
                     /locus_tag="VG754_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18104.1"
                     /translation="ALAAWSIFAIFGWLRRGGFGQKWWRAYVILGLIGLALGIWFAFF
                     LDYKVSNNHLEGFPIPVGISTREKPDAPWKKSDMPVAIRLGGTVTDLLSGIALCLAPI
                     AVAAFFHENRGKGPFASPTAS"
     gene            370..1614
                     /gene="lpxB"
                     /locus_tag="VG754_02515"
     CDS             370..1614
                     /gene="lpxB"
                     /locus_tag="VG754_02515"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWF18105.1"
                     /translation="MTPKRFMIIAGEASGDVLAAELVKELRDQMFRRYQYSSDLQPLY
                     ADLAPRFFGAGGPCMAKADVELAFDLTEHSVTGLWDVLKNYFKFRRLFNQLLAMARER
                     EPHIIICVDFSGFNRRFAAAIKNYVRARRGTFNNWEPKIIQYISPQVWASRADRAYQV
                     ARDVDLLLSIFPFEKDWYAKKVPKLRVEFVGHPLIDRHQGNGARGSQFTGQVMRNPGT
                     NPTIVLLPGSRPGELERHLPPMLDALKILQSSIPNLRARLVLPADSLIAQAKNHSLPT
                     SLEIQCGNLAEALSQADLAIASTGTVTMECAYFGVPTVTIYKALGLSWGFRLGIIKVK
                     WLTMPNILANEELFPEFLQNAATPENIARAALGLLRDPARREKIRSRLSEVIASLGPP
                     GASRRAAHEILKLLAAQMNAGG"
     gene            complement(1808..2125)
                     /locus_tag="VG754_02520"
     CDS             complement(1808..2125)
                     /locus_tag="VG754_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18106.1"
                     /translation="MRILSLLVVMAILGCVTRVLIYISNRTPGPAGFARGMVHGALMP
                     CALPNLLVGNDVTIYAPENTGRTYKLGYTVGVNSCGAIFFGFFFWRLNRWRKKMKASL
                     NSG"
     gene            2200..2928
                     /locus_tag="VG754_02525"
     CDS             2200..2928
                     /locus_tag="VG754_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWF18107.1"
                     /translation="MFLKQRALQAEYFDELDRPPAEIADGYAMLGRVNRFFSFTSPFQ
                     HYLPKMLGEERCRSLSLLDLGAGDGSLGDELSRWATGRGWEWRFINLDLNQQAMRLNP
                     GAKWVAASALRLPFCDQAFDVVVASQMTHHLISEEDACLHFQEAWRVTRQALLLNDLH
                     RNVVLYSALWVLLRLFRFPEHFRNDGLLSVQRSWRVGEWRALAKNAGIPNARVWLYGG
                     ARLMLEARRESAKMVSAKLAMKTA"
BASE COUNT          671 a          852 c          885 g          705 t
ORIGIN      
        1 agcgctggcg gcgtggtcca tctttgcaat ttttgggtgg ttgcgccggg gcggtttcgg
       61 gcagaaatgg tggcgcgcgt atgtcatcct aggtttgatc ggactggcgc tcgggatttg
      121 gttcgccttt tttctcgatt acaaagtcag caacaatcat cttgaaggtt ttcccattcc
      181 ggtcgggatt tccacacgcg aaaagccgga tgcgccctgg aaaaaatccg acatgccggt
      241 tgcgatccgg cttggcggaa cggtgactga tttgttgagc ggcatcgcgc tctgcctcgc
      301 gcctatcgcg gtggcggcat tttttcacga aaatcggggc aagggaccat ttgccagccc
      361 cacagcatca tgactccaaa acgtttcatg atcatcgcgg gcgaagccag cggcgacgtg
      421 ctcgcagccg agctggtcaa ggaattgcgc gaccaaatgt ttcgccgcta tcaatacagc
      481 tccgatctgc aaccgttgta cgcggattta gcgccgcgat tttttggcgc gggcggccca
      541 tgcatggcaa aggcggacgt cgaactggct tttgatctga cggagcattc ggtgaccggt
      601 ctttgggacg tgctgaaaaa ttatttcaag tttcgccgac tttttaatca gttgctcgcg
      661 atggccaggg aacgcgaacc gcacatcatc atttgcgtgg atttctccgg gttcaaccgc
      721 cgcttcgccg ctgcgatcaa aaattacgtc cgcgcgcggc gcggcacgtt caataattgg
      781 gagccgaaaa tcatccaata catctcgccg caagtttggg cttcgcgcgc ggaccgtgcg
      841 tatcaggtcg cacgcgacgt tgatctgttg ttgagcattt ttcccttcga aaaagattgg
      901 tatgcgaaaa aagtgcccaa actgcgcgtg gaattcgtcg ggcatccttt gattgacagg
      961 catcaaggaa acggcgcgcg cggttcgcaa tttactggcc aggtgatgcg caacccggga
     1021 acgaacccga ctattgtttt gctgccgggc agccgaccgg gcgaattgga gaggcatctg
     1081 cctccaatgt tggacgcgct caaaattctg caaagttcca ttcccaatct ccgcgcgcgc
     1141 ctggtgctcc cggccgattc gctcattgcg caggcaaaaa atcattcgct tccaacaagc
     1201 ctcgaaatcc aatgcggcaa tctggcggaa gcattgtcgc aagccgacct cgccattgcc
     1261 tccaccggca ccgtgacgat ggaatgcgcg tactttggtg tgccaacggt cacgatttat
     1321 aaggcacttg gcctgtcatg gggctttcgg ctcggcatca tcaaagtcaa atggctaacc
     1381 atgccaaaca tcctcgccaa cgaagaactt tttcctgaat ttctgcaaaa tgccgccacg
     1441 ccggaaaaca tcgcgcgcgc cgcgttggga ttgctgcgcg accctgcgcg ccgcgaaaaa
     1501 atccggtcca gattatcgga agtcatcgcc tccctcggtc cacccggcgc aagccgtcgc
     1561 gcggcgcatg agattttgaa actcctcgca gcacaaatga acgctggcgg atgaacagca
     1621 catttgaagg attattttga gcgttcgtcg aaattaagca gaggattgat aagatcgcgg
     1681 ttcccctcac acacaatcct gaaattgatg atgatcagcc accagccgaa cagggccgaa
     1741 caaaaccgtc aacactcagg gaaaaatatg gtaagcgtgc atggttcgtg gcctcggagc
     1801 gcgctgctca cccactgttc agcgaagctt tcattttctt gcgccaacgg ttgagccgcc
     1861 agaagaaaaa gccgaagaaa atcgcgccgc aactgttcac gcctaccgtg tagccgagct
     1921 tgtaagtgcg gccggtgttt tcgggcgcgt aaatggtgac gtcgtttccg accagaagat
     1981 tcggcaacgc gcatggcatc aatgcgccat gcaccattcc gcgggcgaat cccgccggcc
     2041 caggagtgcg gttggaaatg taaatcaaca cgcgcgtgac gcagccgagg atggccatga
     2101 ccaccagcag tgaaaggatg cgcaaaagaa ttcttcgcgc ccggccgggc ggcgctgctt
     2161 tgacttgttt ttcgttggtc gtttccgtag ttgataagca tgtttttgaa acagcgcgcg
     2221 ctgcaagcag aatattttga cgagcttgac cggccgccgg cggaaatcgc tgacggctat
     2281 gcgatgctcg ggcgcgtgaa ccgtttcttt tcattcacaa gcccttttca acactattta
     2341 cccaaaatgc tgggcgaaga acgctgccgt tcgctttcgt tgctcgactt gggcgcgggc
     2401 gacggctcgc ttggtgacga actcagccgt tgggcgacgg gacgcggttg ggaatggcgc
     2461 ttcatcaatc tcgatctcaa ccaacaggcg atgcggctga atccgggcgc gaaatgggtc
     2521 gctgcttccg cgttacggct gcctttttgc gaccaggcat tcgacgtcgt tgtcgcgtcc
     2581 cagatgacgc atcatttaat ctcggaagaa gatgcctgcc tgcactttca ggaggcgtgg
     2641 cgggtgacgc gacaggcgct gttattgaat gacctgcata gaaacgtcgt tttgtattcc
     2701 gcgctgtggg tgttgctgcg gttgttccgg tttccggaac atttccgcaa cgacggactg
     2761 ctctccgtgc aacgcagttg gcgggtcggc gaatggcgcg cgcttgcaaa aaacgccgga
     2821 atcccgaacg cgcgcgtctg gctttacggc ggggcgcggt tgatgctcga agcgcggcga
     2881 gaatccgcca aaatggtttc ggcgaagttg gcgatgaaga cggcttaaaa ggtcttcgct
     2941 aattcggtgg aacgcaaact tgggagtttg ttcgaggctc gccctcaccc ctggagaggg
     3001 tgaacatagt tacctcaaag gaacctttga tccgtcctgt tgcaattgcc gctcattgga
     3061 aatcgccgag agagtatctg aaaactgtta gacaaataat tctccctctc cac
//
LOCUS       DASZMM010000100         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1952644, whole genome shotgun sequence.
ACCESSION   DASZMM010000100 DASZMM010000000
VERSION     DASZMM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952644"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VG754_02530"
     CDS             <1..855
                     /locus_tag="VG754_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18108.1"
                     /translation="YLRDEPSADFFPGLAAMASLVQQEAPGKWAYINLFPDYATAGQL
                     GAASYEEYLEKFVAACHPTQLSYDHYALMDDGSLGGGYWNNLEQMRAVAKKHNLPFWN
                     IVLACAHFSFREPTAADLRFEVFSSLAYGARGIAYFTYFAPQVGNYRAAAIDQFGHPT
                     PTWNNLQNVNLQIQKLAPTLLELTSDDTYHFGSVPAGCHGPGTNSLVSAVGGQIAVGD
                     FTHSDGSRYVMLVNKDLSKSVACWPQFRRAPKVLKFISPYTGRSGVYAGEQVWLAPGH
                     GVLLKPEY"
     gene            892..1395
                     /locus_tag="VG754_02535"
     CDS             892..1395
                     /locus_tag="VG754_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18109.1"
                     /translation="MDPIIPPDTHHLLAAIGWLELGNSAEADAELEKIRPALLTHPDI
                     LEVRWQVYAEQKKWALCLEAAKGIVNAAPERSSGYIQRSYALRRIDGRGMEAARDALS
                     EAVPKFPKEPIIPYNLACYECQLGRLQQAMAFLAKAFAMGDPKSLKLMAMNDPDLKPL
                     WTHIREL"
     gene            1424..2296
                     /locus_tag="VG754_02540"
     CDS             1424..2296
                     /locus_tag="VG754_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF18110.1"
                     /translation="MGKLFNTVFLVCALAWHVCSAKADTVSDRITVTVSGKGPDVVLI
                     PGLACSSAVWDGLLKRLDGHYRLHAVQVAGFAGSLSRANAEGPVFQPTADAIDAYIKT
                     NKLKSPKVIGHSMGGLIGMKLALEHPDDVGGLMIVDSFPFFGVLIGVKDAAAAEPRAA
                     AMRDRILNETQEAYAEGEKQFLASMVKSPEGLKIATKWSVESDKSVVARAMYEAMTTD
                     LRPRLHEMKTPVTMLYPWDTSMGASQAMIDGMYQQNFAALPKKTLVRIDGSYHFIMFD
                     QPEKFAEQVSVFLK"
     gene            complement(2365..>2881)
                     /locus_tag="VG754_02545"
     CDS             complement(2365..>2881)
                     /locus_tag="VG754_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWF18111.1"
                     /translation="AVDDLDLQVEIVHRVAKEVMAEKKIKIDYLVGTMIEVPRGALTA
                     NEIAETAQFFSFGTNDLTQTALGISRDDMGSFLQPYVESEIFKKNPFATLDQTGVGQL
                     MKMAAEKGNATRPGIKLGICGEHGGDPDSVKFCHRLGLTYVSCSPFRVPVARLAAAQA
                     ALESEKSAKKK"
BASE COUNT          617 a          770 c          832 g          662 t
ORIGIN      
        1 tatctgcgcg acgaaccgag cgctgatttt tttccgggac ttgccgcgat ggcgtcgctg
       61 gtgcaacagg aggctcctgg aaaatgggct tacatcaatc tgtttcccga ttatgcgacc
      121 gctgggcaac tcggcgcggc gagttacgag gagtaccttg aaaaatttgt cgccgcatgt
      181 catccaacgc aattgagcta tgatcattac gcgctcatgg acgacggttc gctgggaggt
      241 ggctattgga acaatttgga acagatgcgc gccgtcgcaa aaaagcacaa cctgccgttc
      301 tggaacatcg tgcttgcctg cgcccatttt tcattccgcg aaccgaccgc agccgatttg
      361 cgttttgaag tgttcagcag ccttgcttac ggcgcgcggg gcatcgcgta tttcacttac
      421 ttcgcgccgc aagtcggaaa ttatcgcgcg gcggcgattg accaattcgg ccatcccacg
      481 cccacatgga acaatctgca aaacgtcaac ctacagatcc aaaaactcgc gccgaccttg
      541 ctcgaattga cgtccgatga cacttatcat ttcggttcgg tcccggcggg atgccatggg
      601 ccgggcacga acagtcttgt cagcgccgtt ggtggccaga ttgccgttgg cgatttcacg
      661 cacagcgatg gaagccgtta cgtgatgctg gtcaacaaag atctttcgaa gtccgttgcg
      721 tgctggccgc aattccggcg cgcgccgaaa gttttaaaat tcatttcgcc ttacacgggc
      781 cgatccggcg tttacgcagg tgaacaagtc tggctcgcgc ccgggcacgg cgtcttgttg
      841 aagccggagt attgattcgc cagtatctcg ctgtcggtgt ataataccgc cgtggatcca
      901 atcatcccac cggacactca tcaccttttg gcggcaattg gctggcttga actcggcaac
      961 tccgccgagg cggacgcgga gttggaaaag atcaggcccg cgctcctgac gcatccggac
     1021 attttggaag tgcgctggca ggtttatgcg gagcaaaaga aatgggccct ttgcctggag
     1081 gctgcgaaag ggattgtcaa cgctgcgcct gaacgttcga gcggttacat ccaacgctct
     1141 tacgctttgc ggaggattga tggtcgtgga atggaagccg cccgcgacgc gttgtcagag
     1201 gccgttccca aatttccaaa ggaaccgatc atcccttaca acctcgcgtg ctacgagtgc
     1261 cagcttgggc gattgcaaca agcgatggcc tttcttgcaa aagcttttgc gatgggcgat
     1321 cccaaatcgc tcaagttgat ggcgatgaat gacccggacc ttaaacccct ctggacacac
     1381 attcgagaac tctgaatact ggcgcatcac tggttcaaaa attatgggca aactatttaa
     1441 tacggttttt cttgtgtgcg cattggcatg gcatgtctgc tccgcaaaag cggacacggt
     1501 atcggataga atcactgtta ccgtgagcgg gaaaggcccg gatgttgtgc tcattccggg
     1561 actcgcatgt tcgagcgcgg tttgggatgg gttgttgaag cgcctcgacg ggcattatcg
     1621 ccttcatgcc gtgcaagtgg cgggattcgc cggttcgctt tcgcgggcga acgcggaggg
     1681 accagttttc cagccgacgg cagacgccat cgatgcttac atcaagacaa acaagttgaa
     1741 gtcgccgaag gtgatcggcc attcaatggg cggattgatt ggaatgaagc tcgccctgga
     1801 acatccggac gacgtgggcg ggctcatgat cgtggattcg tttccatttt tcggcgtgtt
     1861 gattggcgtg aaggacgccg ccgcggcaga accgagagcc gccgcgatgc gcgaccggat
     1921 tctcaacgaa acgcaggagg cttacgccga gggcgaaaaa caatttctcg cttccatggt
     1981 caaatcaccg gagggcctca agatagcgac caaatggagc gtcgaatcgg acaaatcagt
     2041 cgtagcccgc gccatgtacg aggccatgac gacagacctt cgtcccaggt tgcacgaaat
     2101 gaagacgccg gtgacgatgc tttatccatg ggatacttcc atgggcgcgt cacaggcgat
     2161 gattgacggc atgtaccaac aaaattttgc agcgttgcca aagaagacac ttgtgcgcat
     2221 cgatggttcg taccatttca tcatgttcga tcaaccggaa aaattcgcag aacaggtgag
     2281 cgtgtttctg aaatgattat cgtaaaataa aaaagaccgt ccgattaccg gacggccttt
     2341 gccttgaatc caatctgcga ttggttattt cttcttcgcg gatttttcgc tttccagcgc
     2401 ggcttgcgcc gcagcgaggc gtgcgacggg aacgcggaag ggtgagcagc tcacgtaggt
     2461 caggccgagg cgatgacaga atttcacgct gtcggggtcg ccgccgtgtt cgccgcagat
     2521 gccgagcttg atgccgggac gcgttgcatt gcctttctca gcggccattt tcatgagttg
     2581 gcccacgccg gtctggtcga gtgtggcaaa cgggttcttc ttaaagattt cgctttcgac
     2641 gtacggttgc aggaacgagc ccatgtcgtc gcggctgatg ccgagcgcgg tttgcgtgag
     2701 gtcgttggtg ccgaagctga agaactgcgc ggtctcggcg atttcatttg ccgtgagcgc
     2761 tccacgcggg acttcaatca tcgtgccgac gaggtaatca atcttgattt tcttctcggc
     2821 catgacttcc ttcgccacgc gatggacgat ctcgacttgc aggtcgagat cgtccaccgc
     2881 g
//
LOCUS       DASZMM010000101         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1953119, whole genome shotgun sequence.
ACCESSION   DASZMM010000101 DASZMM010000000
VERSION     DASZMM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953119"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2174
                     /locus_tag="VG754_02550"
     CDS             <1..2174
                     /locus_tag="VG754_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWF18112.1"
                     /translation="VNKATIARQNEPPTPALDLHSDYQVTVNRNDNSALLQTFNLNGT
                     QNQQPLLHADLASPMNFSLGSGSNAKVGDSTLKCVVTNFNLADWKAALGDAASSGTMN
                     MTLNLLSQQGGKQLTVDFNSQLANLTAKVGSNQLQQASMDLNGHLISQQENKALTGNV
                     TLSDFNGTYENYRFQNYGTKVDLDIAMNKPQMEIKNISGTIVEGQNAGGSFKLSGNYN
                     QNDKTGQLMLKLVDWNQNALRPFMGAFLGDKTLESVSLNSTTTANYASSGESSFKTDL
                     QVTNLVVRDPKSQSPPTPLAANLQLDASLNKQVAQVRQLVLKLTPTDQAANNQITATG
                     QLDLSQSNAIQGSLKLASDSLDVTRYYDLYSGKHNAAKPSTSNAAPPAPAQPQTAAAN
                     AEPPAKNLPIKNFTLDASIARLVLHEVNVSNFQTTARVEGSHAVLKPFQFSLNGGPVN
                     ATVDLDLGVPGYKYEMTFSGTNIPLAPLADTFSPEYKNNAKGEVFANLQIKGQGTTGA
                     SMQKTLSGQCDVLLTNANIQVVGAKSKKILTPIAAALKLPELLQSPITWLTADVTIGN
                     GSIKITQFKAAGSAFSASSQGTVAIANVLSNSPIHDLPVNFALAGPLAAKARLMPSNA
                     NTNAAYVDLGKIATVQGTIGDPKTKVDYATIGLITARAAGGIIGGSTGNILKGAGGIG
                     NLLNGQNPPANNQPNQSNTNQSQPNRSSGNPFDLLHKVLPK"
     gene            2307..>3667
                     /locus_tag="VG754_02555"
     CDS             2307..>3667
                     /locus_tag="VG754_02555"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWF18113.1"
                     /translation="MEKVTRYLRDNQPRFVQELSDYVRFASVSAQPQHRKDMLACAEW
                     LKQHCEKIGLEARICETDGHPIVIAKTPRKTNSKRPHYMVYGHYDVQPAEPFELWKSP
                     PFEPRIEGSSLFGRGACDNKGQNLAHFKAVEAYLKTGTELPCDITFVIEGEEEVGSKH
                     LAAFLQGHREELKCDAVVVSDTGIPSPKHPALTYALRGIAALEITLHGPSRDLHSGIF
                     GGSVDNPAMALCQLLAKLRDKNGRITIPGFYDDVAPLSAYEKKQLARLPGNDTAYRKF
                     LGVPQLFGEKGYTSTERRTARPTLEINGLTSGYQGEGSKTIVPAWARAKLTLRLVPNQ
                     NPRRIIQTARKYLTKLCPSTVRIEIASGHGAEPYIVSPTSTQAQAALRALKKAWGYEP
                     VLIREGGSIPIVNDFKKHLHADTLLLGLALPDDNAHSPNEKFDLGCFEKGQLMGAYLW
                     QELT"
BASE COUNT          884 a         1083 c          969 g          731 t
ORIGIN      
        1 atgtgaacaa agccaccatt gcccgccaaa atgaaccacc gactccagcg cttgatcttc
       61 actccgatta tcaagtcacc gtgaaccgga acgacaactc cgccttgctg caaacattca
      121 atttgaacgg cacgcagaac cagcagcccc tgcttcatgc ggacctggcg agcccgatga
      181 atttttcctt gggctcgggc agcaatgcga aggtcggcga ttccacgctc aaatgcgtcg
      241 tgacgaattt caatcttgcg gattggaaag ctgcgttggg tgacgccgct tcatccggca
      301 caatgaacat gacgttgaac ctgctgtctc aacagggcgg caagcaactc accgttgatt
      361 tcaattcgca gcttgcgaac ctgaccgcaa aagtgggcag caaccagctt caacaggcca
      421 gcatggacct taacgggcat ctcatcagcc agcaggaaaa caaggcgctc acgggcaacg
      481 tgaccttgtc ggacttcaac ggaacttacg aaaattatcg tttccagaat tatggaacaa
      541 aggtcgattt ggacatcgcg atgaacaaac cgcagatgga aatcaaaaac atctccggga
      601 cgattgttga agggcagaat gcgggcggct cattcaagct ttcgggcaat tacaatcaaa
      661 atgacaagac gggccagttg atgctcaagc tggtggattg gaaccagaat gcgctgcgtc
      721 cgttcatggg cgcgttcctt ggagacaaaa cgctggaatc cgtttcgctc aattccacca
      781 cgactgccaa ttatgcttcg agcggcgagt catcgttcaa gactgatttg caagtcacca
      841 atcttgtcgt gcgcgatccc aagagccagt cgcctcccac gccgctggcg gcgaacctgc
      901 agcttgacgc ttctctcaac aaacaagtcg cccaggtgcg ccagctcgtc ctcaagctga
      961 ccccgaccga tcaagccgcc aataatcaaa tcaccgcgac cggccagttg gacctttcgc
     1021 aaagcaacgc aatccaaggc tcgctgaagc tcgcttccga ttcgctggac gtgacgcgtt
     1081 attacgatct ttattccggc aaacacaatg ccgccaagcc ttcgacctca aacgccgccc
     1141 ccccggctcc agcgcaacca cagaccgcgg cagcgaacgc ggaaccgccg gcaaaaaatc
     1201 tgccgatcaa gaatttcacg cttgatgctt ccatcgctcg gttggtgctg catgaagtga
     1261 acgtctccaa ttttcaaacc acggctcgcg ttgagggaag ccatgccgtg ctgaaaccgt
     1321 ttcaattcag cctgaacggc gggcccgtca atgcgacggt ggacttggac cttggcgtcc
     1381 cgggttataa atacgaaatg actttttccg gcacgaacat cccgctggcc ccgctcgcgg
     1441 acacgttttc gccggaatat aagaacaatg cgaagggcga agtttttgcc aacttgcaga
     1501 tcaaaggaca gggcacaacc ggcgcgagca tgcagaaaac cctttccggc caatgcgatg
     1561 tgcttttgac caacgcgaac atccaggtgg tcggcgcgaa gtcgaagaaa attttaacgc
     1621 cgattgccgc cgcgttgaag ttgccggaat tgttgcaatc tcccatcacc tggttgaccg
     1681 ccgacgtgac catcggcaac gggtcaatca aaatcacgca gttcaaagcg gccggcagcg
     1741 ccttcagcgc cagcagccag ggcacggtcg cgattgccaa tgtgctttcg aattcgccca
     1801 tccatgatct gccggtgaac ttcgccctcg cgggtccgtt ggcggcaaag gcgcgcctga
     1861 tgcccagcaa cgcgaatacg aacgccgctt atgtggacct cggcaagatt gccacggtcc
     1921 aaggcacgat tggcgatccg aaaacgaaag tggattacgc caccattggc cttatcaccg
     1981 cgcgcgcggc tggcggcatt attggtggtt cgaccggcaa catcttgaaa ggggctggcg
     2041 gcatcgggaa tctgttgaac gggcaaaatc ctccggccaa caatcagccc aatcaatcca
     2101 acacgaatca gtcgcagccc aaccgttcgt ccgggaatcc atttgacttg ctgcacaaag
     2161 ttcttcctaa atagcggcgt gaattttgtt gattcaatgc gcggcgttgg aacgtcgcgc
     2221 gatttttaaa ggcaaggcgg atagcgcgcg ttccctccat tgctccaacg ctccaccaca
     2281 ccaattacca cccattcaac tcacacatgg aaaaagtgac ccgctatctc cgggacaatc
     2341 agccccgttt cgtccaggaa ctttctgatt acgtccggtt cgccagtgtt tcggcccaac
     2401 cgcaacatcg caaggacatg ctcgcctgcg cggaatggct gaaacagcat tgcgaaaaaa
     2461 tcggcctcga agcgcgcatc tgtgaaacgg acggccatcc cattgtcatc gccaagacgc
     2521 cgcgaaaaac aaattccaag cggccgcatt acatggtgta cgggcattac gatgtgcagc
     2581 ctgcggagcc attcgagttg tggaaatcgc cgcccttcga gccgcgcatc gagggcagtt
     2641 cactttttgg ccgcggcgca tgtgacaaca agggccagaa ccttgcgcat ttcaaagcgg
     2701 ttgaagcgta tctgaagacc gggactgaac tgccttgcga catcacgttc gtcatcgaag
     2761 gcgaggagga agttggcagc aaacatctgg ccgcattttt acaagggcat cgcgaggaat
     2821 tgaagtgcga tgccgtggtg gtgtcggaca ccgggattcc cagcccgaaa catcccgcgc
     2881 tcacgtacgc gctgcggggc atcgctgcgt tggaaatcac gctgcacggg ccgtcgcgcg
     2941 atctgcattc gggaattttc ggcggctcgg tggacaatcc cgcgatggcg ctttgccagt
     3001 tgctcgcgaa attgcgcgac aaaaacggac gcatcaccat ccccggcttt tatgacgacg
     3061 ttgcgccgct ttccgcctac gaaaaaaaac agcttgcgcg gttgcccggc aatgacactg
     3121 catatcgcaa atttctcggc gttccgcaat tgttcggcga aaagggctac acttccaccg
     3181 agcgccgcac cgcgcggcca accctggaaa tcaacgggct taccagcggt tatcagggtg
     3241 aaggcagcaa gacgattgtg cccgcatggg cgcgcgcgaa gctgacactt cggttggtgc
     3301 cgaatcaaaa tccgcgcaga atcatccaga cggcccgcaa atatttgacg aagctatgcc
     3361 cgtccacagt gcggatcgaa atcgcctctg gtcacggcgc ggaaccgtat atcgtctcgc
     3421 ccaccagcac acaggcacaa gctgcgttgc gcgcgcttaa aaaagcgtgg ggctacgagc
     3481 cggtcctcat tcgcgaaggc ggctcgattc cgatcgtcaa cgatttcaaa aaacatcttc
     3541 acgccgacac gctgttgctg ggccttgcgc ttccggatga caacgcgcat tcgccgaacg
     3601 aaaagtttga tcttggttgt ttcgagaaag gccagttgat gggcgcgtat ctttggcagg
     3661 aattgac
//
LOCUS       DASZMM010000102         4446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2791403, whole genome shotgun sequence.
ACCESSION   DASZMM010000102 DASZMM010000000
VERSION     DASZMM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791403"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..629)
                     /locus_tag="VG754_02560"
     CDS             complement(54..629)
                     /locus_tag="VG754_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWF18114.1"
                     /translation="MSLTPSTMLPLGTAAPDFRLPDTNGKIVSLADFKNAPALLVMFI
                     CNHCPYVKHIRLGLAQLGRDYQSKGVGIVAINSNDTANYPADCPAKMKEEAKSAGYMF
                     PYLFDETQNVAKVYRAACTPDIFLFDAGQKLVYRGQFDDSRPGNGKPVTGQDLRAALD
                     AVLRGQPAPTDQKPSIGCNIKWKSGNAPDYF"
     gene            complement(641..1612)
                     /locus_tag="VG754_02565"
     CDS             complement(641..1612)
                     /locus_tag="VG754_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18115.1"
                     /translation="MSDFKFSCPKCGLHVVCDTSNAGMQIPCPGCQTLLTVPQPPQAA
                     TPAAPAPGRLTINKSAHQSHAPQPAPGAANAPAKPAWGAKPQPAPRAKKKSPWVPIIV
                     VVAILAVAAIGWFGFGAPYLAKQAKQKQDALEAQQKAEQETKEAAAEAARAKKASWKL
                     DLAGAKFPERHASGKHNGADFTVENTVFQGGALVLLQTTGTPRQFVISIPLKTGETLS
                     GKTFNVASTATNAVQPRVVLNWRDDPAKAPSKETFAKGYAMKLEFGTLADSKLPGKIY
                     LCVPDAEQSFVAGNFQIGPKTASPTSVAPAAPAAVDPNAGRAPLRKL"
     gene            complement(1726..3123)
                     /locus_tag="VG754_02570"
     CDS             complement(1726..3123)
                     /locus_tag="VG754_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HWF18116.1"
                     /translation="MNQPAFAEFETKMRKAGSSDAAIRAFKHSYESLAAGKTGLIPEY
                     SIQPVTRLPRFEDIAKKSSPDSSLLSQAVVLKLNGGLGTSMGLERAKSLLRIKDDLTF
                     LDFITKQILYLRQKHDCPLRFLLMNSFSTSRDTLEFLKKYPALGEPAQLELMQNAVPK
                     VDAQTLRPVAWPQNPELEWCPPGHGDLYPSLLGSGWLDRLLADGVKYLFVSNSDNLGA
                     SLDLNLLAWFAASNQAFLMEVCERTSSDRKGGHLAQSNGRLLLRESAQCPEEDMAAFQ
                     DIQRHRFFNTNNLWIRLDELKKLLDAHGGFIPLPIIKNAKTVDPRDKKSPKVFQLETA
                     MGAAIECFDRSGAIVVPRIRFAPVKTTADLLALRSDAYEVTEDWRLLLSEAGHGVPPA
                     IDLDADHYKLVDQLDASLKEGVPSLMHCRELTVRGPVLFNSRNIFIGKVSLANSSTAP
                     KPVPAGEYKNVAKEL"
     gene            complement(3196..>4446)
                     /locus_tag="VG754_02575"
     CDS             complement(3196..>4446)
                     /locus_tag="VG754_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HWF18117.1"
                     /translation="GTHYGIPFPVFCRAAFGTVGANVPALLRALVACGWFGIQTWIGG
                     NAIYKILGVFIPSLTNAPVLGALGINLPQLICFLFFWAINIWIIYLGIDSIRLLLSIK
                     APLLIALGLSLLGWAYHYAGGFGPILSQPSAFAPGQPRSGEFFKTFVPALTGMVGFWA
                     TLSLNIPDFSRYARSQRDQVIGQALGLPVTMALYAFIGVAVTSATTIIYKQTIWDPVD
                     VLTRFTNPFVLIIAMLALCIATLATNIAANVVSPANDFAHIAPRHISFRTGGLITGII
                     GVCIMPWKLLADPSGYIFTWLIGYSALLGPIGGILIADYFVLRRRQLNLAALYDPVGE
                     YRYTNGFSIIGLVALVLSILPNLPGFLVAIHKWPKDWALPAFLVNLYNYAWFVGFLLA
                     FVIYLALKSLALARHPAAAVKPVS"
BASE COUNT         1005 a         1157 c         1324 g          960 t
ORIGIN      
        1 ctgctgattt tggcctgggt ttgggcgctg ctgattgtga ccaagccgaa ggctcagaaa
       61 taatccggcg cgttgccgga tttccatttg atgttgcagc cgatgcttgg tttctgatct
      121 gtcggtgcag gctggccacg caggacagca tcaagcgcgg cgcggagatc ttggccggtt
      181 accggcttgc cgttgccagg ccggctgtcg tcgaactgcc cgcgataaac gagcttttgc
      241 ccggcatcga aaagaaaaat gtcgggcgtg caggcggcgc gatagacttt ggcgacattt
      301 tgcgtttcat cgaacagata ggggaacatg tagcccgccg atttggcttc ctctttcatc
      361 ttcgcgggac agtctgcagg ataattcgcg gtgtcattgg aattgatggc aacgatgccg
      421 acgcccttcg attggtaatc gcggcccaat tgcgccaagc ccaggcggat gtgctttacg
      481 tagggacaat gattgcaaat gaacatcacg agcaaggcgg gcgcgttttt aaaatctgcc
      541 agcgaaacaa tttttccatt cgtgtccggc aaccggaaat ccggcgcagc ggtgcccagc
      601 ggcagcatgg tggaaggggt gagtgacatg ctttggggtt ttaaagtttt ctcagcggcg
      661 cacgtccggc attcggatcc acggccgccg gtgctgcagg cgccaccgac gtcgggctcg
      721 cagtcttggg gccgatctga aaatttcccg cgacaaaact ttgttccgcg tcgggaacgc
      781 aaagataaat cttcccgggc aacttgctgt ccgcaagggt cccgaattca agtttcatgg
      841 catagccttt tgcgaatgtc tccttcgacg gcgcttttgc cggatcgtcc cgccaattca
      901 gcacaacgcg cggctgaacc gcattcgtgg cggtcgaagc gacattgaat gtcttgcccg
      961 aaagggtttc gccagttttg agcggaatcg agatgacgaa ctgccgcgga gtgccggtgg
     1021 tctgaagcag cacgagagcg ccgccttgga acacggtgtt ttccactgtg aaatcggcgc
     1081 cgttatgttt gccggaagcg tggcgctcgg gaaattttgc accagcgaga tcaagtttcc
     1141 aggaagcctt ttttgccctg gccgcttcgg cagccgcttc ttttgtttcc tgttcggcct
     1201 tctgttgcgc ctccaaggcg tcttgttttt gtttggcttg ttttgccaga taaggcgcgc
     1261 cgaacccaaa ccatccgatt gcggccacgg ccaaaatcgc gacaacgacg atgatgggca
     1321 cccacggaga tttctttttc gcccgcggag ctggttgagg ttttgcgccc caagccggct
     1381 tggcgggggc attggccgca ccgggagctg gctgcggcgc atgagattga tgcgcggact
     1441 tgttgatcgt cagcctgccg ggagcgggag cggccggagt ggcggcttgc ggcggctgcg
     1501 ggactgtgag cagggtttga cagccggggc agggaatctg catcccggcg ttgctggtgt
     1561 cacagacgac gtgcaggccg catttcgggc aggaaaattt aaaatcgctc atgaatgttg
     1621 aggggaattt ccatcaaaga atcaaattca acaagtccga agatgcgttg gcgtattgga
     1681 gtgatggaca gttgggaaga cacaaggggc cattgctgct ttcattcaca attccttcgc
     1741 gacgtttttg tattcacccg ccgggactgg ttttggagcc gtggatgagt tggcaaggga
     1801 gactttgccg ataaaaatat tgcgtgagtt gaagagcaca gggccgcgca ccgtcagttc
     1861 gcggcaatgc atcaatgaag gcacaccctc cttgagcgag gcatcgagtt ggtccaccag
     1921 cttgtaatgg tcggcgtcga ggtcaatcgc aggcggcacg ccatgtccag cctcggacaa
     1981 caacaaacgc caatcttcgg tgacttcgta ggcgtccgag cgaagcgcga gcaggtcggc
     2041 agtggtcttc accggcgcga aacggatgcg cggcacaacg atcgctccag atcggtcaaa
     2101 acattcaatg gccgcgccca tggcggtttc gagttggaaa acctttggtg attttttgtc
     2161 gcgcgggtca acagtcttcg cgtttttgat gatgggcagc ggaatgaacc cgccatgcgc
     2221 gtcgagcaat tttttgagtt catccaggcg tatccaaaga ttgttggtgt tgaaaaaacg
     2281 atggcgctgg atgtcctgaa atgcggccat gtcttcctcg ggacattgcg cggattcgcg
     2341 gagcaacagg cgtccgttgc tctgtgcaag atggccgccc ttgcggtctg aagaggtgcg
     2401 ttcgcaaact tccatgagga acgcctggtt ggaagcggcg aaccatgcga gcagattgag
     2461 gtcaagactt gcgccgagat tgtcggaatt ggaaacgaaa agatatttca cgccgtcggc
     2521 aagcaggcga tcgagccaac cggaaccgag caatgaggga tatagatcac catgaccggg
     2581 cgggcaccat tcgagttcgg gattttgcgg ccatgcgacg ggacgaagcg tttgcgcgtc
     2641 cactttggga actgcgttct gcatcaattc gagttgggca ggttcgccca aagccggata
     2701 ttttttgaga aattccagcg tgtcgcggct ggtgctgaag ctgttcatca acaggaaccg
     2761 cagcggacaa tcgtgtttct gacgcaggta aagaatttgt ttggtgatga aatcgaggaa
     2821 cgtcagatcg tccttgatgc gaaggagcga tttggcgcgt tcgagtccca tgctcgtgcc
     2881 gagtccgcca ttgagcttga gaaccacggc ttgtgaaagc aacgagctgt cagggctgga
     2941 ttttttggcg atgtcttcga accgcggaag cctggtgacg ggctggatgc tgtattctgg
     3001 aatcaagccg gttttgccgg cggcaaggct ttcgtaactg tgtttgaaag cgcggatggc
     3061 ggcatcgctg ctgccggctt tgcgcatctt ggtttcaaat tcagcaaacg caggttgatt
     3121 cataatcttc gcaaattgtt catggcgcga ggcgttttgt ccatggggta tggatgcgca
     3181 cagggtaacg cgtattcagg aaaccggctt gacagcggct gccgggtgcc ttgccaacgc
     3241 caaacttttc aacgccagat aaatcacaaa tgccaaaagg aaacccacga accatgcata
     3301 attgtagaga ttcaccaaaa atgccggcag cgcccaatct ttcggccatt tgtggattgc
     3361 caccaaaaac cccggcaaat tcggcaagat gctcaacacg agcgcgacga gcccgatgat
     3421 gctgaagccg tttgtgtaac gatattctcc tacagggtca tacaacgcgg ccaagttcaa
     3481 ttgccggcgg cgcagaacaa aataatccgc gatcaagatg ccgccaatcg gccccaaaag
     3541 agcgctgtaa ccgatcagcc aggtgaaaat gtatcccgac gggtccgcga gcaatttcca
     3601 cggcatgatg cacacaccga tgatgccggt gatcaaaccg ccggtgcgaa atgaaatatg
     3661 ccgcggcgca atgtgcgcga aatcattcgc gggactcacc acattggccg cgatgttcgt
     3721 cgcgagtgtc gcgatgcaca acgccagcat cgcgatgatc aacacgaacg gattcgtgaa
     3781 tcgcgtgagc acatccaccg ggtcccaaat cgtttgcttg taaatgatgg tcgttgccga
     3841 cgtgaccgcg actccgatga acgcatacaa agccatcgtg actggcaatc caagcgcttg
     3901 cccgatcact tggtcgcgtt gggagcgcgc gtaacgcgaa aaatccggga tgttcaacga
     3961 cagcgttgcc caaaaaccga ccatgccggt caacgccgga acgaaggtct tgaaaaattc
     4021 gccgctcctg ggctgaccgg gcgcgaatgc ggaaggttgc gaaagaatcg gcccgaaacc
     4081 acccgcataa tgatacgccc agccgagcaa cgacaatccg agcgcgatga gcagcggcgc
     4141 cttgatgctg agcagcagcc ggatcgaatc gattccgaga tagatgatcc agatgttgat
     4201 ggcccagaaa aacagaaagc aaataagttg cggtaaattg attcccaatg caccgaggac
     4261 aggcgcattt gtcagcgatg gaataaagac gccgagaatt ttgtaaatcg cgttgccgcc
     4321 aatccatgtc tggatgccga accatccgca cgccaccaat gcgcgcaaaa gtgccgggac
     4381 gtttgcgccc acagtgccga atgccgcgcg gcaaaaaacc ggaaacggaa tcccataatg
     4441 cgtgcc
//
LOCUS       DASZMM010000103         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3059393, whole genome shotgun sequence.
ACCESSION   DASZMM010000103 DASZMM010000000
VERSION     DASZMM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3059393"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VG754_02580"
     CDS             <1..1401
                     /locus_tag="VG754_02580"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HWF18118.1"
                     /translation="PNWEFKFLNRALEEDPQVQMVSLLRMARREPKFEFKGKSGESSN
                     PLFRGFGDTNEETARYDQPVIVRLNTKDEFELRGSFPKTAEELFRYHAVIIGDLEAEF
                     FTHDQMVLLQKFVSERGGGFMMLGGAESFREGNYAGTVIASMLPVYLDQPANAKLPSQ
                     WKFTLTREGWLQPWARLRATEADERTRLDSMPTFPVLNSVRGIKPGASVLATVSDAEN
                     NTYPALAVQRFGLGRTAAMMIGDLWRWGMLDEAAQKDRAKSWRQLVRWLVSDVPARVA
                     VMPETSNGGDSVRLVVTAKDEAFKPVDNANVQLTIRPARVFDSQGNGALNAVSETNYV
                     QLTAEASPNQPGTYEATYIAREAGAYTVAVNATQSDGKEIGRTSAGWTSDPAAEEFRS
                     LKPNRALLESIAQRTGGKVLKMDELADFARHLPERRAPITEPWSDPLWQKPLVYLFVL
                     GCFVTEWGIRRWKGLP"
     gene            1398..>2205
                     /locus_tag="VG754_02585"
     CDS             1398..>2205
                     /locus_tag="VG754_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18119.1"
                     /translation="MRLGSFKKVTFSIVFCIIAFCGSSHAQTNEQRSLILVIGAPGAP
                     EYAEEFSASAALWQAAANKGGLQVSVIGQNDTIEDDRTRLLNVLASEVSKPAGELWLV
                     FLGHGTFDGKTAKFNLRGPDISAEELATALKPCRRPLVVIQCASASGPFLKALSGTNR
                     VVITATRSGYELNVTRFGSFMARAIADSTADLDKDGQTSLLEAFLMASRQVEQFYKDQ
                     GRLLTEHALLDDNGDGLGTPPDWFSGLRAVKKAADGKSVDGVRANQMILVR"
BASE COUNT          472 a          582 c          672 g          479 t
ORIGIN      
        1 ccgaattggg aattcaaatt tctcaatcgc gcgcttgaag aagacccgca ggtgcagatg
       61 gtttcgctcc tccgcatggc gcgtcgcgaa ccgaagttcg agttcaaggg aaaatccggc
      121 gagtcgagca atccactctt ccgcggtttc ggcgacacca acgaagagac cgcccgttac
      181 gaccagccgg tcatcgtgcg cctgaacacg aaggatgaat tcgaattgcg cggcagtttt
      241 ccaaagacgg ccgaggagct ttttcgttat cacgccgtca tcatcggcga cctcgaagct
      301 gaatttttca cgcacgacca gatggtattg ttgcagaaat ttgtctccga acgcggcggc
      361 ggttttatga tgcttggcgg cgcggaatcg tttcgtgaag gaaattatgc cggaacggtg
      421 atagcctcca tgctgcccgt ttatctcgat caaccggcca atgccaaatt gccttcgcaa
      481 tggaaattca cgttgacgcg cgaaggctgg ttgcagccgt gggcgcgatt gcgcgccacc
      541 gaggcggatg aacgaacgcg tctggattcg atgccgacat ttccggtgtt gaattccgtg
      601 cgcggaatca agcccggcgc gagcgtgctg gcgacggttt ccgacgcgga gaacaacact
      661 tatcccgcgc ttgccgtgca acggtttggc ttgggacgca ccgcggcgat gatgatcgga
      721 gatttgtggc gctggggaat gctcgacgaa gcggcgcaaa aggaccgcgc caaatcctgg
      781 cgtcaacttg tccgttggct ggtgtcagat gtgccggcgc gcgtggccgt catgccggag
      841 acttcaaacg gcggagattc cgtccgtctt gttgtcaccg cgaaggacga ggcgttcaag
      901 cccgtggaca acgccaatgt tcaactcacc attcgtcctg cgcgggtctt cgattcgcag
      961 ggcaacggcg cattgaacgc tgtttctgaa accaattatg tgcaactcac ggcggaagcc
     1021 tcgccgaacc agccgggaac ttatgaagcc acttacatcg cgcgcgaagc gggagcgtac
     1081 acggtggctg tcaatgcaac acagtctgac ggaaaagaaa tcgggcgcac atccgcaggc
     1141 tggacatcag acccggcagc cgaggaattt cgttcgctga aaccgaatcg cgcgttgctg
     1201 gaaagcatcg cacaacggac cggaggcaaa gttttgaaga tggacgaact tgcagatttt
     1261 gcgcgccatc tgcctgagcg tcgcgcgccc atcaccgagc cgtggagtga tcccttgtgg
     1321 caaaagccgc tcgtctattt attcgtgctg ggatgttttg tgactgagtg gggaatccgc
     1381 cgctggaaag gattgccgtg aggttgggga gtttcaaaaa agttactttc agcatcgtat
     1441 tttgcattat tgcgttctgc ggatcttccc acgcgcagac caatgaacag cggtcgctca
     1501 ttcttgtcat cggcgcgccg ggcgcacccg aatatgccga ggaattctcc gcatcggcgg
     1561 cgttgtggca agcagcggcg aacaagggcg gtttgcaagt ctcggttatc gggcaaaacg
     1621 acaccatcga agatgatcgc acgcgtttgt tgaatgttct tgcgtcggaa gtttcgaaac
     1681 cggcaggaga actttggctc gtttttcttg ggcacggcac ctttgacggc aagacggcaa
     1741 aattcaattt gcgcgggccg gatatttcgg cggaagaatt ggcaacggca ttgaaaccgt
     1801 gtcgtcgtcc gctcgttgtc attcaatgcg cgtcggcgag cgggccgttt ctcaaggcgc
     1861 tttccggcac gaaccgggtc gtcatcactg cgacccggag cgggtatgaa ctcaatgtga
     1921 cccgattcgg cagcttcatg gcacgcgcga ttgctgactc tacggctgat ttggacaagg
     1981 acggtcagac ttctttgctg gaagcttttt taatggcgtc gcggcaggtg gaacaattct
     2041 ataaggacca gggccggttg ctgaccgaac acgctttgtt ggatgacaac ggcgacgggc
     2101 tcggcacgcc acccgattgg ttcagcggat tgcgggctgt caaaaaggcg gcggacggaa
     2161 aatcagtgga tggagttcgc gcgaaccaaa tgattttggt tcggg
//
LOCUS       DASZMM010000104        10967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3059466, whole genome shotgun sequence.
ACCESSION   DASZMM010000104 DASZMM010000000
VERSION     DASZMM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3059466"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            651..983
                     /locus_tag="VG754_02590"
     CDS             651..983
                     /locus_tag="VG754_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18120.1"
                     /translation="MFARHLTVSSVALLVASDGLFHNAGNLSMFFCTTTEFPMQNSSK
                     PKNKKKPIEVLTVLKGDKLAGKHRNKLLEDAKLENQVLYFDLEQAECGGANNVSNISA
                     LSRSGGLG"
     gene            1003..2727
                     /locus_tag="VG754_02595"
     CDS             1003..2727
                     /locus_tag="VG754_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWF18121.1"
                     /translation="MQLLQDVAKARLSIKSGTVEFEIFHHDFGPAYPGTNHTHVKAVF
                     DGINRRFESSRREFAYVLLGPEGAAATDAKMKELGLDQEGAVRAGLLRGFESHHMTAY
                     DGATLMDYWTTDGKPFQTKVDDPKKGSVEFVFDPRILGIAYGPFSTETVSNCLDTGNA
                     GSVQLVGNESVDGKDAWHVRIARNSRFELDFWIDAHEPRHVLKFVCNTQIVESKYDSS
                     RPDDPLPIEVALTSLDGKRKPRYETRYVRQFTQYNIPVDPASWTLAGLGMQIGTEVVD
                     YRISRRLGYWDGSGLSENLPSRPHREIRNESPPPNPDKLLALIEKDPKSSFALNAAIW
                     IILNTPDGPQVEQAAAVILKEHIRSTNLVQLCQELNRMHPRCAKELLQAALDKNPGPE
                     VKAQACFALAMLLKGEANERGDEQLSKEAQKLFERVIADYGEVKLEGKPLSDAALPKL
                     TELRLLAVGKVAPEIEGENLEGQKMKLSDFRGKVVVLTFWGTWCGSCMEMVPDERKLV
                     SRLTGKPFALIGVNSDDNGTKLKSVLEQKKITWPSFRDGQTPGRIAAAWNVQSWPAIY
                     VLDTKGVI"
     gene            2808..3380
                     /locus_tag="VG754_02600"
     CDS             2808..3380
                     /locus_tag="VG754_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HWF18122.1"
                     /translation="MKSPDIDPKQTALVLIDLQQGIVNRPGAPRSGPEIVKNAARLAG
                     KFRALNATVVLVHVGFHPDLKDRLDVPVDAPANFGALPPNWAEFVPEIGPQPGDLIIT
                     KHQWGAFYGTELDLQLRRRGVRMIALGGIATNFGVESTARDAYERGYHQIFVEDAMTS
                     VSAEAHEFVVKNIFPRIGQVRSTDEVLSAL"
     gene            3402..4439
                     /locus_tag="VG754_02605"
     CDS             3402..4439
                     /locus_tag="VG754_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWF18123.1"
                     /translation="MLTPFDLLQTPQTDPTSIYRHRDGLYAVDMLTCAISHLDFFSWL
                     AEHPVGRSAICKGLNLHERPTDVMLTLFTAMGLIECKDAVFQLTMLAREHLVKTSPWF
                     IGPYYDSMKERPVCKDLLEVLRTGHPANWGSFKHEKDWAKAMEGEAFANQFTAAMDCR
                     GTLLAPAMAAKVDCSRRKHLLDIAGGSGIYACAMAARHLHLRATVMEKPPVDRITKNQ
                     IAARGFAERIDVKVGDMFADAFPPQCDAHLFSNVLHDWDEPRVRQLLKKSFAALPADG
                     LIVIHDAHINADKTGPLPVAAYSTLLMAITEGKCYSEKEMADYLHDAGFKKVAFTPTV
                     ADRSVIVAEKS"
     gene            complement(4445..4780)
                     /locus_tag="VG754_02610"
     CDS             complement(4445..4780)
                     /locus_tag="VG754_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18124.1"
                     /translation="MKVIIQDIGAREFLGADGQWVSDKEDARDFFTLLHAYHFAQANT
                     SIHFRVLLHCPEDDYSASIIEGIGTAGEEASIFRVPIKKASVKPAKQPVNPALLFART
                     FATARVHLN"
     gene            5214..5861
                     /gene="hpt"
                     /locus_tag="VG754_02615"
     CDS             5214..5861
                     /gene="hpt"
                     /locus_tag="VG754_02615"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWF18125.1"
                     /translation="MKNAFAVPSIRSQPVPARWRTEVERVLISERQVASRIRELSVEI
                     QRDFHGREMVVVSLLNGTVMFLADLIRHLSVPLRLDFMGVSSYGLGTESGDLVFTKEL
                     RLDVRGRDVLLVDDILDTGKTLSKVLAKLRVLKPRRIKTCVLLDKAARRVEPVQADYI
                     GFRIPDFFVVGYGLDFAERYRNLPFVGVLHPQIYKQVSKRAIRTPRNKSRKRSQK"
     gene            5899..6144
                     /locus_tag="VG754_02620"
     CDS             5899..6144
                     /locus_tag="VG754_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWF18126.1"
                     /translation="MQVSVHFYSYFKDLAGCDQTVVMVADNSTLDDLLRELSVRFPKW
                     AAMKNCALTAVGVEYQGRDYVLKAGDEVSLFPPVQGG"
     gene            6153..6548
                     /locus_tag="VG754_02625"
     CDS             6153..6548
                     /locus_tag="VG754_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWF18127.1"
                     /translation="MKRELVITAEPINETVLVARRVMSGGMGAAVYFTGVVRDAEDGA
                     AIKAIEYEAFQQMAEHQFHLLFDEIEKRWPIESVRLVHRIGLVKVNEPSLWVEIVAPH
                     RGEAFAACEWLIDEMKRVVPIWKKPISSN"
     gene            6886..7353
                     /locus_tag="VG754_02630"
     CDS             6886..7353
                     /locus_tag="VG754_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18128.1"
                     /translation="MKHSVAIVVLIASLLLGLAMNSRAQTYSINWYKVAGGGGTSTTG
                     SYALSGTIGQPDAGTMSGGAYTLTGGFWGLIATVQIAGAPFLSLWTTNSHNAVLSWPA
                     SATGFHLQQDPSLGNTNWSSVNTNTYPINLSNGTNYVTLPISGANQFFRLSNP"
     gene            complement(7398..7547)
                     /locus_tag="VG754_02635"
     CDS             complement(7398..7547)
                     /locus_tag="VG754_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18129.1"
                     /translation="MATSKKIPARSKQTESIRAVVSLPAPLPFLIAQRNLFVPKAHSL
                     AEGAD"
     gene            complement(7798..9681)
                     /locus_tag="VG754_02640"
     CDS             complement(7798..9681)
                     /locus_tag="VG754_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF18130.1"
                     /translation="MKPKATKYLSVALAGILQILPVVRSALPGLQAIASSPTGALILR
                     WVAGGVAYFGYHAISGATTLNYAISISPPTATQGKFYSGTLTWTGGYSGEVSSMSING
                     NCIVGTPYTISPGLSIIYSGGSTATISGTPMTSNLFTLTSEIYLYGNCTASSIYEATR
                     TSPAFTVISSNGLPVGPIFNNYPQNEVAILGSPVNLFGSATGIPSPAYQWSRNLAPIS
                     GATNAALAITDAEITNAGTYSLMASNSTSHPSAICVLSVCLPPGTNFTTLYWTNYAVA
                     GVPLTMTSFITNVSTATNSTKWSFNLGPPILTNQNFALTAGQVTPNKSGTYSAFFNSV
                     NGTNVYANGIEFDSQWMFGYLPVITNQPTPVTVRAGSNATFTCTVAGGNFANIFLYQN
                     GTNLVAQTNFLDYNPGGYGTNGNLQPGNASTTTNISLTVSNVTLANAGNYTFVITNFW
                     GSTTSSPVALTVNAPPTVSSPLGQTNYAGKTVNVGVTSTGTAPLNFQWQKGGANVSNG
                     GNISGALTNILTFSPAALANSGNYQVIVTNTSGSVTSTVAVVSIVPVPPFALSLGAGN
                     AALSASGGVAGSNYIVQMCTNLASNSWTNLYTNSVPANGNISFTDANAPTNGQRYYRV
                     QFP"
     gene            complement(9701..>10967)
                     /locus_tag="VG754_02645"
     CDS             complement(9701..>10967)
                     /locus_tag="VG754_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWF18131.1"
                     /translation="FPEKRTFVLNTNLALSKMFTEIMPTVTPAILPPADSDWAKQLNG
                     FKVAARQSGYLPTKKFLSFLERAQSGNDSVDDPLARFQHAGLMVTLLLIVVGGILLNL
                     TPCVLPMIPINLAIIGAGSEASSRVVGFKNGGIYGAGMALAYGTLGLAVVLTGAKFGA
                     INSSIWFNVVIAVVFVVLAFAMFDKINIDFSRFSGSLGPQKTNAKRGSVIHHMVIFIM
                     GAMAALLAGACVAPVVISVMLLAANIYSKGHIAGLLLPFLLGAGMALPWPLAGAGLTF
                     LPKPGVWMTRVKHGFGVLILVFGIYYAHLAVAAYQSSIDSTTSLASAPAASASARDDA
                     NLELAKALQTAKQDGRPVLVDFHASWCKNCLAMDETVFRKNEVKQRLQDFILVRYDAE
                     RPGEYPAKEVLDHFGVLGLPSYVVLTAEK"
BASE COUNT         2468 a         2796 c         3185 g         2518 t
ORIGIN      
        1 caatgacggt gccttttacg atttcgccag cggcaaagcg catttcgctg tgcttcatgg
       61 cttcttccat tgtgagcata atgattctat gatgaacgga ttctgaggga gacttggtca
      121 agcgcggagg cgctctcgaa ggctccattg cctaactgac cagacgttgc agggcaacgg
      181 aggttgaggg ttaggttatt cgttaacttc tttttctcag ccctaaacga acagccgcac
      241 atacggcgtt caggcaggcg ataacatacg ctcggcaagg atggttgcaa gaccaaagtg
      301 gcggggattg ggagttggag tgatgaagtc ataggttgat tggtcaaggc ttgaccggcg
      361 tagaataatg gcttgactag ccttgactcc atttaggctg aaaatgatgt taattcattg
      421 acggagaatg ggttgtatcg attttcgcat ggcttgacca gtaacgttaa aggcttgact
      481 cgctgaggaa atagagtggt ggaatgaggg agtgatgaac cttggacttg gggttgcgtc
      541 tttgatgctg gatttacagc cgacccggaa ctcggaaccc gtacctcggg accgtgctgc
      601 acaacgcgca tcttggacaa aagagatgtt ttccggcgga tgattgaccg ttgttcgcca
      661 gacacctgac ggtgtcctct gttgcgctcc ttgtcgcatc ggacggattg tttcataacg
      721 ccggaaatct gtccatgttt ttctgcacaa caaccgagtt cccgatgcag aattcttcaa
      781 agcccaaaaa caaaaagaaa ccgattgaag tgttaacggt cttaaaaggt gataagctgg
      841 ctgggaaaca ccgaaacaag ctattggagg atgcaaaact tgaaaaccag gttttatact
      901 tcgatttgga acaggctgaa tgtggcggcg caaacaacgt tagcaatata tctgctctca
      961 gccggagcgg tggtttgggc tgatgatcaa aacccaaatc cggtgcaatt gttgcaagat
     1021 gtcgccaagg cgcgattgag catcaaatca ggcactgtgg aattcgagat ttttcatcat
     1081 gactttggtc ccgcttatcc cggaacgaat catacccatg tcaaagcggt ctttgatggc
     1141 atcaatcgcc ggtttgaatc ttcccggcgg gaatttgctt acgtgctcct tggaccggaa
     1201 ggggccgcag ccaccgatgc gaagatgaag gaattgggac tggaccagga aggcgctgtg
     1261 cgggcgggat tgttgagagg gttcgaatcg caccacatga ccgcgtacga cggcgcaacc
     1321 ttgatggact attggaccac cgatggcaaa cctttccaaa ccaaagtgga tgacccaaag
     1381 aaaggcagcg tggagttcgt gtttgatccg cgcatcttgg gcattgcgta tggtcctttt
     1441 tcaaccgaaa cagtgtccaa ctgcctcgat accggcaacg ccggctccgt tcaactggtg
     1501 ggcaacgaat cggtggatgg aaaagatgcc tggcatgtgc gcatcgctcg aaattcccgt
     1561 ttcgaattgg atttctggat cgatgcgcat gaaccgcggc atgttctcaa gttcgtctgc
     1621 aatacccaga tagttgagtc caaatatgat tcctcccgcc cggacgaccc gcttccaatc
     1681 gaggtcgccc tgacatcttt ggacgggaaa cgaaaacccc gctacgaaac gcgctacgtt
     1741 cggcagttca cacagtacaa tattcccgtt gacccagctt cgtggacgct ggccggattg
     1801 ggaatgcaaa tcggcacgga agtggtggat taccgcatca gccgacgctt gggatattgg
     1861 gatggaagcg gcctttcgga gaatctccct tcgcggccac atcgcgaaat aagaaatgaa
     1921 agcccgccgc caaacccgga caaattgctc gcgctcatcg aaaaggatcc caaatcgtcc
     1981 tttgccctga acgctgccat ctggatcatt ttgaacacgc ccgatgggcc gcaagtggaa
     2041 caagccgccg ccgtcattct caaagagcat atccgcagca caaacctggt ccagctttgc
     2101 caggaattga accgcatgca ccctcgttgc gccaaggagt tgctccaagc agcgctcgac
     2161 aaaaatccgg gccctgaagt gaaggcgcag gcttgtttcg cgctcgcgat gctgttgaaa
     2221 ggcgaggcca atgaacgcgg cgatgaacaa ctttccaaag aggcgcagaa attatttgag
     2281 cgcgtcatcg ccgattatgg agaagtaaag ttggagggca agccgttgtc ggacgctgcc
     2341 cttcctaaat tgacggaatt gcggctgctt gcggtgggca aagttgcgcc ggaaattgaa
     2401 ggcgagaatc tggaggggca gaaaatgaaa ctgagcgatt ttcgcggcaa ggtcgtggtg
     2461 ctgacgtttt gggggacatg gtgcggttcg tgcatggaaa tggtccccga cgaacgcaaa
     2521 ctggtctcgc gcctgactgg aaaacctttc gcgttgatcg gcgtcaactc ggacgataat
     2581 gggacgaaac ttaagtcggt tttggagcag aaaaaaataa catggccgtc atttcgagac
     2641 ggccagacgc ccgggcgaat tgccgctgcg tggaacgtgc aaagctggcc cgcaatttac
     2701 gttctcgaca cgaaaggagt catctgattc cggaatttaa gagggcagcc tcttgctgac
     2761 gctgtggaca aattgctcgc cgaatagaca gtaaaatata ggattttatg aaatcgcccg
     2821 atattgaccc gaaacagacg gccttggtac tgattgatct ccagcaaggg atcgtaaatc
     2881 gccccggcgc tccgcggtcg ggcccagaaa ttgtcaaaaa cgcggcgcgc ttggctggca
     2941 agtttcgcgc cctgaatgcc acggtcgttt tggtgcatgt cggttttcat ccggatttaa
     3001 aagacaggct ggatgtcccg gtggacgctc cggccaattt tggcgcgctt ccacccaatt
     3061 gggcggaatt cgtgcccgag atcgggccgc agccgggcga tcttatcatc accaagcatc
     3121 aatggggcgc gttttacgga accgaactcg acctgcaatt gcgacggcgc ggagtgcgga
     3181 tgattgcgct tggcggcatc gcaacaaact tcggcgtcga atcaactgct cgcgacgcct
     3241 acgagcgggg ttatcatcaa atttttgtgg aagatgccat gaccagcgtg agcgcggagg
     3301 cgcatgagtt cgtggtaaaa aacatctttc cgcgcatcgg acaagtccgt tccacggatg
     3361 aagtgctttc cgcgttgtga gcatctcgtt acattcgttt catgctcaca ccctttgatt
     3421 tgttgcaaac gccgcagacg gatccaactt ccatttaccg ccatcgcgat gggctttatg
     3481 cggtggacat gctgacgtgc gcgatttcgc atctggattt cttttcgtgg ctcgcggagc
     3541 atccggtcgg ccggagcgcg atttgcaagg gcttgaatct tcatgaacgc ccgacggatg
     3601 tgatgctcac gttgttcacg gcgatggggt tgatcgaatg caaggacgcc gtttttcaac
     3661 tcaccatgct ggcgcgcgaa catttggtga aaacgtcccc atggttcatc gggccgtatt
     3721 acgattcgat gaaggaacgg ccggtctgca aggatttgct ggaggtgttg cgcaccgggc
     3781 atccggcgaa ctggggcagt ttcaagcatg aaaaggattg ggccaaagcg atggaagggg
     3841 aagcgttcgc caatcaattc actgccgcga tggattgccg cggcacattg ttggcgcccg
     3901 cgatggctgc gaaggttgat tgttcgcggc gcaagcatct gctcgacatc gcgggcggct
     3961 cggggattta cgcttgcgcg atggcggcgc ggcacttgca cttgcgcgca actgtgatgg
     4021 aaaagccgcc ggtggaccgc atcacgaaaa atcaaattgc cgcgcgcggt tttgccgaac
     4081 gcattgatgt gaaggtgggc gacatgtttg cggacgcgtt cccgccgcaa tgcgatgcgc
     4141 atcttttttc aaatgtgttg cacgattggg atgaaccgcg cgtgcggcag cttttgaaga
     4201 aatcttttgc cgcgctgccg gccgacggtc tcatcgtcat ccacgacgcg catatcaacg
     4261 ccgataaaac ggggcctttg cccgtggcgg cttattcaac gctgctgatg gccatcaccg
     4321 aaggcaagtg ctactcggaa aaagaaatgg cggattacct gcacgacgca ggctttaaaa
     4381 aagttgcatt cacgccgact gttgccgata gaagcgtaat cgtcgcagag aaaagttgag
     4441 ggactcagtt caagtgaacg cgcgcggtcg caaaagttct ggcgaacagc aacgcgggat
     4501 tgacgggttg cttcgccggc ttgaccgatg ctttttttat tggcacgcgg aagatggatg
     4561 cttcttcgcc cgccgttcca atgccttcaa tgatgctggc ggagtagtcg tcctccggac
     4621 aatgcaacag cacgcgaaag tgaatggaag tgttcgcctg cgcgaaatgg taggcgtgca
     4681 gcagcgtgaa aaaatcccgc gcgtcttcct tgtcggaaac ccattggccg tccgccccga
     4741 gaaattccct ggcgccgatg tcttgtatga tcactttcat cgaaaattct atcggcaaga
     4801 aacatgccaa cccctcgcaa aatgcggtgt ttgatgccaa atcgtttttc atggtgagcg
     4861 aggaggattg agcgggaaat tttgtaagtc aaatcggaaa tgcgcggttc aagatttccg
     4921 acgcgcttgg acactgtgtc tcgttgcgtc taatttttga acagttacgt gcgcatcttg
     4981 gcactgttcc tgattgagga ttttggatgc aagaatccag gcgactggaa agtcgcctga
     5041 acccgcagac aagaatgtct gcgctacgtg gcgcaggctt tccagcctgc gggttgcggg
     5101 gactttccag tccccgctcc agaacggcat aaagatacgc gaacagttac tcaccgctgg
     5161 acaatgccgg ctgtctccta ttattcttgc agaatgtctg atcgccgggc ttgatgaaaa
     5221 acgcatttgc agtccccagc atccgttcgc agccggttcc cgcgcgctgg cgcacggaag
     5281 ttgagcgcgt gttgatcagc gaacggcagg ttgccagccg catccgcgaa ctctcggtgg
     5341 aaatccagcg cgattttcac ggacgcgaaa tggtcgtggt ttcgttgctg aatggcacgg
     5401 tgatgttcct tgccgatttg atccggcatc tttcggtgcc gttgcgattg gatttcatgg
     5461 gtgtttccag ttacggtctt ggcacggaat cgggcgatct tgtcttcacc aaggaattgc
     5521 gccttgatgt gcgcggacgc gatgtgctgc tggtggatga tattttggac accggcaaaa
     5581 cgctgagcaa agtgctggcc aaactgcgcg tgcttaaacc gcgccgcatc aagacttgcg
     5641 tgttgctcga caaggcggcg cgtcgcgtcg agccggtgca ggccgattat attggctttc
     5701 gcatcccgga ttttttcgtg gtgggttatg gattggattt tgcggaacgc taccgcaacc
     5761 tgccgttcgt cggcgtgctg cacccgcaga tttacaaaca agtttccaag cgcgccatcc
     5821 gcactccgcg caataaatcc agaaagcggt cacagaaatg aactccgcac tccgcattcc
     5881 gcactccatt cttgtttgat gcaggtttcc gtccattttt attcttattt caaggatttg
     5941 gccggttgcg accaaaccgt tgtcatggtc gccgacaaca gcacgcttga cgatttgctg
     6001 cgcgaattgt cagtgcgatt tcccaaatgg gcggcgatga aaaattgcgc gctgaccgct
     6061 gtgggtgttg aataccaagg acgcgattac gtgttgaagg ccggggatga agtttcgctt
     6121 ttcccgccgg tccaaggcgg ctgaatcttc acatgaaacg cgaactggtc atcactgccg
     6181 agccgatcaa tgaaactgtc ttggtcgcgc ggcgtgtgat gagcggcgga atgggcgcgg
     6241 cggtgtattt caccggtgtg gtgcgtgatg ctgaagatgg cgcggcgatc aaggcaatcg
     6301 agtatgaagc gtttcaacag atggccgagc atcaatttca cctgctgttc gacgagattg
     6361 aaaagcgctg gccgattgaa tcggtgcggc ttgtgcatcg catcgggttg gtgaaggtca
     6421 acgagccgtc gctttgggtg gagatcgtcg cgccgcatcg cggcgaagca tttgcggcat
     6481 gtgagtggct gattgacgag atgaagcgcg tcgtgccgat ttggaagaag ccgataagct
     6541 caaattgaaa gtgcggctcg cgaggtcttt ccacacaggc aacgctcacc tcacccgctt
     6601 tttggcgcat ccgcgaatgc cccgatttgt tggccatacc cggacggacg gactgaacta
     6661 ttgcgcgcca tgttcactgg acgaaacgga gcgcggctcg gtgagccgca gcgacacgcg
     6721 acaaacagga cagtgccaga ctaatttatg cgcgcctgtc atttccacat tgctgcgggt
     6781 caccacccgc gctccgggcc aatgcgcgga tgtgcttccc gcgttttgat ttggcccaaa
     6841 attgcttgct tttttgcgtt cattttggga aagttaaagc cagccgtgaa acattcagtt
     6901 gccatcgttg ttttgattgc cagcctcttg ctgggtttgg caatgaacag ccgcgcgcag
     6961 acctacagta tcaactggta taaagtcgcg ggcggcggcg gaacgagcac cactggcagt
     7021 tacgctctct ccggcaccat cggccaaccg gacgccggaa cgatgtccgg cggggcatat
     7081 actttgacgg gcggtttttg gggcttgatc gcgacggtgc aaatcgctgg cgcgccgttc
     7141 ttgtcccttt ggactacgaa ttcgcacaac gctgttttgt cctggccggc ttccgcaact
     7201 ggttttcatc tccaacagga cccgagcttg ggcaacacga attggtccag tgtgaacacc
     7261 aacacgtatc ctatcaacct ttccaacggc acaaattacg tgaccctgcc catcagcggc
     7321 gcgaatcaat tcttccggct gtcgaatccg taagacaaaa tcaatcagag gttcgtcgca
     7381 ctacgcttgc tttcaattca gtccgcgcct tcagccaatg aatgtgcctt gggcacgaac
     7441 agattgcgtt gcgcgatcag aaacggcaga ggcgcgggca aagaaacgac tgcgcgaatg
     7501 gattcggttt gtttggagcg ggccggaatt tttttgctcg tggccatttt cgaaaatttg
     7561 gtttcggtct tgcttttgcg cataactaat aaatgtaacc aaccttgagg gactcgtctc
     7621 tggggtaaaa tgcccgttcg gcaatcttcg actggaaaaa aatggtgaac ctttccatac
     7681 accttggagt aatggagcgc tgctgtgcgc gaagcgccag ccgcaagcat gtaactgaaa
     7741 tccggcatgt tccaagttgc ggctggtcct gcggacacag ccgcgctcca ctattattta
     7801 tgggaactgc acgcgataat accgctggcc gttggtcggc gcgttggcat cagtgaacga
     7861 gatgttgccg tttgcgggca cactattcgt gtaaagattg gtccaactgt tggaggcgag
     7921 attggtgcac atttgcacga tataattgct cccggcgaca ccgcccgagg cactcaaggc
     7981 ggcgttgccc gccccaagtg agagcgcgaa tgggggcact ggcacgatgg acacgaccgc
     8041 caccgtgctt gtcacgctac cagaagtgtt ggtgacgatg acctgatagt tgccgctgtt
     8101 cgcaagcgcg gcgggactga acgtgaggat gttcgtgagc gccccggaga tgttgccgcc
     8161 gttggacacg ttggccccgc ctttctgcca ttgaaaattt aatggagcgg tgccggtgga
     8221 agtaacgccc acattgaccg ttttccccgc gtaattggtt tggccaaggg gcgagcttac
     8281 ggtgggcggc gcattgacag tcaacgcgac aggcgaactg gtcgtcgaac cccagaaatt
     8341 ggtgatcacg aaggtataat tgccggcgtt ggccaaggtc acgttggaaa ctgtcaggct
     8401 gatgttagtt gtcgtgctgg cgttgccagg ttggagattg ccgttcgttc cgtaaccgcc
     8461 agggttgtaa tccaagaagt ttgtttgagc aacaagattg gttccgtttt gataaagaaa
     8521 aatattcgca aaattcccgc ctgccacagt gcaggtgaat gtggcgttgg aaccagctct
     8581 gacggtcacg ggcgtcggct ggttggtgat gaccggcaaa tagccaaaca tccactgact
     8641 gtcgaactca atgccgttgg cataaacatt cgtgccgtta acgctgttga aaaacgcgga
     8701 ataggtgccg ctcttgttcg gtgtgacttg accggcagta agggcaaaat tttggtttgt
     8761 caatatgggc ggacccaaat tgaaagacca tttcgtgctg ttggtggccg tcgaaacatt
     8821 tgttatgaag ctggtcatgg tcagcggcac ccccgcaacc gcgtaattcg tccaatataa
     8881 tgtcgtgaaa ttagttcccg gcggcaggca gacgctcagg acgcaaatcg cgctgggatg
     8941 ggaagtggag ttgctcgcca tcagcgaata agtgcccgca ttcgttattt ctgcgtcggt
     9001 tatggccaac gccgcattgg tcgcccctga aatcggggcg aggttccggc tccattggta
     9061 cgctggagat ggaatgccgg tggcgctgcc aaaaaggttc acgggagagc cgaggattgc
     9121 aacttcattt tgcgggtaat tgttgaaaat gggacctacc ggcaagccat tgctagaaat
     9181 gacggtaaat gcaggagatg ttctcgtagc ctcatagata ctgctggctg tgcaattacc
     9241 gtagagataa atttccgaag ttaaagtaaa aaggttgctc gtcattggtg tgccagagat
     9301 tgtcgcggta ctgccaccgc tgtaaatgat gctcagtccc gggctgattg tgtatggagt
     9361 ccccactata caatttccat ttatgctcat ggagctgact tcgccagaat aaccgcccgt
     9421 ccaagtcagt gtgccgctgt aaaacttgcc ctgtgttgcc gtcggtggac tgatggaaat
     9481 tgcgtaattg agggtggtag ctcccgaaat ggcgtgatag ccgaaataag ccacgccgcc
     9541 ggcgacccag cgcaagatga gcgcgccggt cggggacgat gcaatggctt ggagtccggg
     9601 aagggctgag cgcaccacgg gcaggatttg caaaattccc gccagcgcga cggaaagata
     9661 ttttgttgct ttgggtttca tgggttgtgg tttttgggtt ttacttttcc gcggtcagga
     9721 ctacgtagct gggcaggccg agcacaccga agtggtcaag cacttccttg gcgggatatt
     9781 cgccgggccg ctcggcgtcg taacggacga gaatgaaatc ttgcaggcgt tgcttcactt
     9841 cgttcttgcg aaaaacggtt tcgtccatcg cgagacagtt cttgcaccag cttgcgtgaa
     9901 aatccacgag caccggacgg ccatcctgtt tcgcagtctg gagcgccttg gcaagttcca
     9961 aattcgcatc gtcccgcgcc gaggcggatg cggcaggcgc ggatgcgagc gaggtggtgg
    10021 agtcaattga actttgataa gccgcgaccg ccagatgcgc gtaataaatg ccaaagacga
    10081 ggatgaggac cccgaagcca tgtttcaccc gcgtcatcca gacgccgggc ttgggcaaga
    10141 aggtgagtcc cgctccggcc agcggccagg gcaaagccat tcccgcgccg agcaagaacg
    10201 gcaacaacaa tccggcgatg tggcccttgg aatatatgtt cgccgccagc agcatcacgg
    10261 aaatcaccac cggcgcgacg cacgcgcccg cgagcaatgc agccatcgcg cccatgatga
    10321 agatgaccat gtggtggatg acgctgccgc gttttgcatt ggttttttgc ggccccaagc
    10381 tgccgctgaa ccgcgagaaa tcaatgttga tcttgtcgaa catcgcgaac gccagcacca
    10441 cgaataccac ggcgatcacc acgttgaacc agatggagga attgattgcg ccaaacttcg
    10501 cgccggtcag caccacggcc aatccaagcg ttccatacgc gagcgccatg ccagcgccat
    10561 agatcccgcc gttcttgaat ccgactacgc gcgaactggc ctcactgccg gcgccgatga
    10621 tggcgagatt gattggaatc attggcagta cgcatggagt caggttcaac aaaattccgc
    10681 ccaccacgat caataataac gtgaccatca ggcccgcgtg ctggaaacgc gcgagcggat
    10741 cgtccacgct gtcgttgccg gattgcgcgc gttccaggaa ggaaagaaat ttttttgtgg
    10801 gcagataacc ggattggcgc gctgcgactt tgaaaccgtt caattgcttt gcccaatcgg
    10861 agtccgcagg cggcagaatg gcgggcgtca cggtcggcat aatttctgtg aacatttttg
    10921 ataaagccag attggtgttg agcacgaagg tgcgtttctc cgggaaa
//
LOCUS       DASZMM010000105         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5251048, whole genome shotgun sequence.
ACCESSION   DASZMM010000105 DASZMM010000000
VERSION     DASZMM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5251048"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VG754_02650"
     CDS             <1..629
                     /locus_tag="VG754_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18132.1"
                     /translation="SPQVAIRVSNPAAVRFDLALVTFSATAKFDLSLLDAVGKSDLRN
                     ITIEGDILSAISAPELAFFALDPRAKPGVVLPQDNVAGVEVSGTIPVDVIQVGGIEAV
                     AFGTLLGPDGKPVSVQSVVGSHLDPGVLSNLLGNSTAFYSPTDTLRLPSLAGLNSALY
                     VYSSANQLMNYANAFVNQVADEGSLTTFVNIATAKQFGTALLRTTLPP"
     gene            748..1971
                     /locus_tag="VG754_02655"
     CDS             748..1971
                     /locus_tag="VG754_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWF18133.1"
                     /translation="MKMNLWTAGLVAMGAVSVASVASADDSANSVLTSLSSSTLSGYV
                     DTSAQWNLGTGDKNAPAYAFGGASKADGFNLNVVKLTLEHAPDANDGWGAGYKVDLLF
                     GPDADALGTQSTGVAGDFGIKQAYVDLKVPVGNGLDFKVGVWDTIIGYESFESVNDPN
                     FTRSYGYTIEPTTHTGVLGTYQFCEAASGSVGVANTFGPAINSRAFSPTGAPGFQAES
                     YKTYMGSLAITAPTNWGWVGGSTLNAGIINGFNIKSPVTINQGTGHADQTSYYAGITL
                     NTPLSALKFGAAYDYVIVGTEHEFGVKASRANAAGIYAAYQLCEKCSINARGEYFFQT
                     KQNATAGLPSKVLEGTLTLQYDLWKNVLSRVEFRWDHQADGTGLAYGAPSAEPILHGS
                     LRNSYELIANIAYKF"
     gene            2195..>2503
                     /locus_tag="VG754_02660"
     CDS             2195..>2503
                     /locus_tag="VG754_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18134.1"
                     /translation="MGMGNGNKSGFTLVELMIVVAIIGLLAAIAIPNMVHARTQSQTN
                     ACINNLRQIDGAKQEWAMEHPVKGTARPGIAELQPYMGRGSQGSLPTCPLDTQNSWDT
                     S"
BASE COUNT          544 a          699 c          694 g          566 t
ORIGIN      
        1 gcagcccgca agtcgccatt cgtgtgtcga atcccgccgc ggtgcgcttt gaccttgcgt
       61 tggtgacctt cagtgcgacg gcgaaatttg atctctcgtt gcttgatgcg gtgggcaaat
      121 cggatttgcg caacatcacc atcgaaggcg acatcctgag cgccatcagc gctcccgaat
      181 tggcgttctt cgccttggat ccgcgcgcaa agccgggtgt cgtgctgccg caggacaacg
      241 tcgccggcgt ggaagtgagc ggaacaatcc cggtggatgt catccaggtg ggcggcatcg
      301 aagcggtcgc gttcgggacc ttgctcgggc cggatggcaa accggtgtcg gtccaatccg
      361 tggttggctc tcatcttgac ccgggcgtgc tttccaatct gcttggcaac agcactgcat
      421 tctactcgcc gactgacaca ctccggctgc cttcgctggc cggcttgaac agcgcgcttt
      481 atgtttacag cagcgcgaac cagctcatga attacgcgaa tgcgttcgtc aaccaggttg
      541 ccgacgaagg ttcgttgacc acatttgtga acattgccac cgccaaacag tttggaactg
      601 cgctgctgag gaccacgctg ccgccgtgag gaggcccgtc aaatttcttg cctcctggtg
      661 aacggtcgcg catgatgcgc gcaatcaatg acggatgcgt tcacattaat cagaattgaa
      721 caccttaaac ctatcaccaa acatttcatg aaaatgaacc tctggaccgc cgggctcgtc
      781 gcgatgggcg cagtcagtgt tgcttccgtt gcttcggcag atgacagcgc caactccgtc
      841 ctgacctcgc tttccagttc cactttgagc gggtatgtgg acacctccgc gcaatggaac
      901 cttggcaccg gcgacaaaaa tgcacccgcc tatgctttcg gcggcgcgag caaggcggac
      961 gggttcaacc ttaacgtggt gaagcttacc ttggaacatg cgcccgacgc gaacgacggt
     1021 tggggcgccg gttacaaagt ggacctgctt ttcgggcctg atgccgacgc attgggcacg
     1081 cagtccaccg gcgtcgcagg cgatttcggg atcaagcagg cttatgtgga tttgaaagtg
     1141 ccggtgggca acggccttga tttcaaggtc ggcgtttggg acacgatcat cggctacgaa
     1201 tcgtttgagt cggtaaacga tcccaatttt acccgctctt acggttatac catcgagccg
     1261 accacgcaca ccggcgtttt gggaacgtac caattttgtg aagcggccag cggctctgtg
     1321 ggcgtggcga atacttttgg gcccgccatc aattcacgcg cattttcgcc caccggcgcg
     1381 cccggctttc aggcggaatc gtacaagact tacatgggct cgctggcgat cactgcgccg
     1441 acgaactggg gatgggttgg cggttccacg ctcaatgccg gcatcatcaa cggcttcaat
     1501 atcaagtctc cggtgaccat caaccagggg acgggccatg ccgatcaaac cagttattat
     1561 gcgggcatca ctttgaacac gcctctctcg gcgctcaagt ttggagcggc gtatgattat
     1621 gtcatcgtcg gcaccgagca tgaatttgga gtgaaagcct ctcgcgccaa tgctgcgggt
     1681 atttatgccg cataccaact ctgcgaaaag tgcagtatca atgcccgagg cgaatatttc
     1741 ttccagacca aacaaaatgc gaccgccggt ttgccttcca aagtcctcga aggaactctt
     1801 actctgcaat atgatctctg gaaaaatgtg ctcagccggg ttgaattccg ctgggaccat
     1861 caagcggacg gaacgggatt ggcatacggg gctccttccg cagaaccgat tcttcacggc
     1921 agtcttcgca attcctacga actcatcgca aatattgctt acaagttctg agcgggaata
     1981 acgcggcaga attgtgtgtt gagaatcgca gctttcgctt caaagtggga catttttgtc
     2041 ctgatttgag gcaacctatc ccaaaagctg gagtggtatt ccgaatgctt tctcagtcaa
     2101 ggtgggcagc ggtgatttaa ttatcagaaa gcgaaatcat tgttgttaca aaaatcatcc
     2161 gctgcccgcc acgatttttg ggaagggttg agttatggga atgggaaatg gaaacaaatc
     2221 gggattcact cttgtcgagt tgatgattgt ggtggcgatc atcgggctgc ttgccgcaat
     2281 cgccatcccc aacatggtcc atgcccgcac acagtcgcag accaacgcct gcatcaataa
     2341 tctgcgccag attgacggcg ccaagcagga atgggccatg gagcaccctg ttaaaggcac
     2401 ggcaagaccc ggcattgcgg aattgcaacc ttacatggga cgcggctcgc aaggttccct
     2461 tccgacctgt ccgctcgaca cccagaattc ctgggacacc agt
//
LOCUS       DASZMM010000106         6025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4168844, whole genome shotgun sequence.
ACCESSION   DASZMM010000106 DASZMM010000000
VERSION     DASZMM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4168844"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VG754_02665"
     CDS             <1..1294
                     /locus_tag="VG754_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HWF18135.1"
                     /translation="TVIAGSNLVYTITVTNLGPYNSSNVVVSDNLPLNVTFVSASGGG
                     IDTSGVVTWPHITSLTNGAVTNFTVTIKAPASGSFTNSASATASTIDPNPANNNGTGA
                     ASRASTTVIPAISADIAVFDSGLTNVTAGKNLTYTITVTNLGPSTATNVVVSDFLTTN
                     VTFVSASGNGTNKSGTVTWKIGNLTNGAASNVTVTVTVPVSASGSITNIASGTNNTFD
                     PNAANNDGSDTNSQVATTITPPQFGILAGTNILNPQTGLYEEHVVVTNIGATTVLGVR
                     LYVDGLRSGVQLYNASGTNAGRPYAQYNSSLNPNQTVAFTLEFYNPSRLPFTNSLDAE
                     AITNPLTFVTNSAGSTPITRAFMDTRTAPPRFVIEFTTVPGKSYTIIYSDDNMQTWKV
                     ATPSITAGSNSTQWYDDGPPETDVAPAGGRFYAIIANP"
     gene            1303..2358
                     /locus_tag="VG754_02670"
     CDS             1303..2358
                     /locus_tag="VG754_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18136.1"
                     /translation="MNYRSFAWFTLAAAPFALLAPKTAFAQDDGSTTNLNHFRLSARV
                     GFNITGRFKNLGSLTLTPSGRTTPNGDPYNYDNGYVLTDVSGSAGGQTWYWGYDGPGQ
                     ISGNTILMTRSTPSGNTEAPGGIGSGDPDPGFEFVYDRELGSHGKLHYGVEAAINYTR
                     VSFNDHSAFAGNVTHRTDAYPFTPGTTPPATPPPYQGTYDGPGFLLGATPISSSTVTT
                     GGAIITGQREFDSDIIGFRLGPYVEYPLTQKLDLSVSAGLAVGFLMNSASWNEVITIG
                     GNQAATSSGSGNNTAFVLGGYISADLSYQFSKRWSAEGGVQFQDLGTYQQNVGTRAIE
                     LDLSKSLFVTLSVGYHF"
     gene            2577..5237
                     /locus_tag="VG754_02675"
     CDS             2577..5237
                     /locus_tag="VG754_02675"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWF18137.1"
                     /translation="MTDMTRSLKTCAFLSAAILSGIFQSPAAPAKEKNLVQDGGFEQG
                     GAGWAFSVSGANASGQAVTGEAHSGKKSFRISNNSGFAPNVYGRVTQMVRGLEPFTTY
                     RISCFARGTNAGIVWIGGGPGWYLRAPFPKGTFGWTNVSFEYATGEEPPDFELMVLTE
                     SQTEALWVDDISMTPIKSDTAKRDAVLNQITGQWKARQDRLSSLQARAGQVPNSRNDS
                     VMQLGFNVAGRFLKRTERGANALQGWAWTRLQLEEIATVLDATENRLNALAKPVEPPV
                     AQPWPKNGPVQLRDGLFEAKMKNGAEQPFWFYGYGHFGQVIRDLPNFRGLGASLVQDG
                     QVGPSSMNADGSFGGSAIQLFKDLRTARRNGMRIDWLLSPHYFPDWAFAKSPDARGGG
                     LGFIGFDIDHPTTREVLENFTGAITEKMKDDDALFTVCLSNEPVYDKSGRTKYGRSEF
                     DAYLENIHGRIGQLNVLYGTRYTNFSEVAPPANGLKATVNENRAYYDWVRFNQRHFAA
                     WHAWLGSLVKKHLPSTPTHAKIMVFYCLDRDKLHFGVDPELFCDATDLAGCDAYAFPT
                     GDKTYDWMGQEFFYDLLHSFKNQPVFNSENHVIPDGAAPDDVPLATARAQFWQGGLHH
                     QAATTCWVWEEAIDTSLSGSIYFRPANIYGAGRAMLELNRFAAEAAAINRLQARIALL
                     YSPASIFWEDAYKGTIQSCYRQLNFLGEPVDFISEKEFAEGRMPTNEFIIAPAATHVT
                     DATAKNLRRFVETGGHLLLAGAGNFAFDEYHRARPTSEIPAGLQLPSVAQEQAAADAL
                     REILANNGIAVGNVIDNATGKPAWGIEFRQATVNGNPVIAMIALDGQNHSASIPALHG
                     KKVVDVLANELIDPDKINLEPMTPHLLEMR"
     gene            complement(5392..5721)
                     /locus_tag="VG754_02680"
     CDS             complement(5392..5721)
                     /locus_tag="VG754_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF18138.1"
                     /translation="MAEIIWTEPALGDLDGIADYIALDKPDAASRLVRRVFERVELLA
                     KHPELGPRIPELSRNSLYRQLVESPCRIFHRYERRAKKLYILGVVRGEKLFEKRLLKE
                     RDSRTRQ"
     gene            complement(5709..5966)
                     /locus_tag="VG754_02685"
     CDS             complement(5709..5966)
                     /locus_tag="VG754_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWF18139.1"
                     /translation="MKIELVTTLKRKATDIIASVAKSRDPVLITQHGRPAAYVVDVET
                     FEGMNEKLAVLETISRGERAIETGRILSHEAAKKRMARWLK"
BASE COUNT         1362 a         1773 c         1641 g         1249 t
ORIGIN      
        1 gacggtcatt gccggttcca atctggttta cacaatcacc gtgaccaatt tggggcccta
       61 taattcgagc aatgttgtcg tgagcgataa cctcccgctc aatgtgactt ttgtgagcgc
      121 gagcggcggt ggaatcgaca ccagcggcgt ggtcacatgg ccgcacatca cgagcctgac
      181 caacggcgcg gtgaccaatt tcacagtcac aatcaaggct cccgccagcg gttcattcac
      241 caacagcgcg tcggcaacgg cttcgacgat tgatcccaat cccgccaaca acaacggcac
      301 cggcgccgcg tcgcgcgcaa gcacgacggt catcccggcc atctccgcgg acatcgcggt
      361 attcgattcc ggcctgacga atgtcactgc cggcaaaaac ctgacttaca ccatcaccgt
      421 gacaaatctc ggtccttcga cagccaccaa tgttgtggtg agtgatttcc tcacaaccaa
      481 cgtcacgttc gtgagcgcca gcggcaatgg cacgaacaaa tccggcacgg tcacttggaa
      541 gatcggcaat ttgaccaacg gcgcggccag caatgtgacg gtcacggtga ccgttcctgt
      601 gagtgccagc ggttcgatca cgaacattgc gtccggcacc aacaacacgt ttgatcccaa
      661 cgccgccaac aacgacgggt cggacaccaa ttcgcaggtg gcaaccacca tcacgccgcc
      721 gcagtttgga attttggccg gcacgaacat tttgaatccg cagaccggcc tctatgagga
      781 acacgtcgtg gtgacaaaca taggcgcgac gaccgtcctt ggagtgcgtc tctatgtgga
      841 tggattgcgc agcggcgtgc aactttacaa cgcctccggc acgaacgcag gccggcctta
      901 cgcgcaatac aattcgtcat tgaacccgaa ccaaaccgtt gctttcacgc tggagtttta
      961 caatccgagc cgcctgccgt tcacgaacag cctcgatgcg gaagccatca ccaacccgtt
     1021 gaccttcgtg accaactccg ccggttccac gcccatcacg cgcgcgttca tggatacgcg
     1081 cacggcaccg ccgcggttcg tcatcgaatt caccacggtt ccgggcaagt cgtacacgat
     1141 catttacagc gatgacaaca tgcagacatg gaaagtcgcc accccgagca tcaccgcagg
     1201 ttccaacagc acgcaatggt acgatgacgg gccgcccgaa accgatgtcg cgccggctgg
     1261 cggacggttt tacgcaatca tcgcgaaccc ttgatcatga atatgaatta ccgatctttt
     1321 gcctggttca ccttggccgc cgcgccgttt gcgctgctcg ccccgaagac tgcgttcgcg
     1381 caggacgatg gctcgacgac gaacttgaac catttcaggc tttcggcgcg cgtgggtttc
     1441 aacatcaccg gccgcttcaa aaatcttggc agcctcacct tgacgcccag cggccgcacg
     1501 actcccaacg gcgacccgta taattatgac aatggatatg tgctgaccga tgtgagcggc
     1561 agcgccggcg gacaaacttg gtactggggt tacgacggac ccggccagat ctccggcaac
     1621 acaatcttga tgacgcggtc aacgccgtct ggaaacacgg aagcacccgg cggcattggc
     1681 agcggcgatc ctgatcctgg atttgaattt gtctatgacc gcgaactcgg ttcgcacggc
     1741 aagttgcatt atggcgtcga agccgccatc aactacacac gggtcagttt caatgaccac
     1801 agcgcatttg cgggcaacgt cactcacagg acggatgctt atccgttcac tcccggcaca
     1861 acgcctccgg ccacgcctcc gccttatcaa ggcacatacg atggtcccgg ctttttgctc
     1921 ggcgcaacgc caatcagctc gtcaaccgtg acgaccggcg gcgcaatcat cactggccag
     1981 cgcgaatttg attccgacat aatcggtttt cgtttggggc cgtacgtcga atatccgctg
     2041 acacaaaaac tggatctttc cgtgtccgcc ggtttggccg tggggttctt gatgaattcc
     2101 gcgtcatgga atgaagtcat caccatcggc ggaaaccagg cggcgacgag ttccggcagc
     2161 ggcaacaaca ctgctttcgt gttgggcggc tatatcagcg ccgatttgtc gtatcaattt
     2221 tcaaaacgct ggagcgcgga aggcggcgtg caatttcaag atttgggaac gtatcaacag
     2281 aacgtcggca cgcgcgccat cgaattggac ttgagcaaat cattgttcgt gaccctttcg
     2341 gtcggctatc atttttaagc ggctccaagc aatctcatcc cacgtggagc gctgtgcgca
     2401 aagcgccagc cgcagcaagc cacagaaatc ccaagtgttc caagctgctg cgactggtac
     2461 tgcagacaca gtcggtcttc ataaagattt tctttctaga tgggcagcca caagtcaaaa
     2521 gaaaattagt ttcttgatta gatcgggaga ccgggcattt ttgtccgcgt aaaatcatga
     2581 ctgacatgac ccgctctctc aagacgtgcg cttttctttc ggctgcaatt ttgtccggaa
     2641 ttttccaatc acccgccgcg cctgcgaaag aaaaaaacct cgtgcaagat ggcggattcg
     2701 aacaaggcgg cgccggctgg gctttcagcg tcagcggcgc gaatgcgagc ggccaggcag
     2761 tcacgggtga ggcgcacagc ggaaaaaaat cttttcgcat ctccaacaat tccgggtttg
     2821 cgcccaatgt ctatggccgc gtgacgcaaa tggtccgcgg cttggagccg ttcacgactt
     2881 atcgcatcag ttgtttcgcg cggggaacaa atgcgggcat cgtctggatc ggcggcggac
     2941 cgggttggta tcttcgcgcg ccttttccca aaggaacatt cggttggacc aatgtatcgt
     3001 ttgaatatgc cacgggcgaa gaaccgccag atttcgaatt gatggtcctg accgaaagcc
     3061 aaaccgaggc gctctgggtg gatgatattt cgatgacgcc aatcaagtcc gacaccgcca
     3121 agcgcgatgc cgtgctgaat caaatcaccg ggcaatggaa agcgcggcag gaccgccttt
     3181 cctcattgca agcgcgcgcg gggcaagtgc caaattcacg aaacgattcc gtcatgcaac
     3241 tcggcttcaa tgtcgccggg cgttttttga aacggaccga gcgcggcgca aacgcattgc
     3301 aaggctgggc ttggacgcgt ttgcaattgg aagaaatcgc gactgttctc gatgccacgg
     3361 agaatcgttt gaacgcattg gccaaacccg tcgaaccgcc ggtcgcgcag ccatggccaa
     3421 aaaacggccc ggtgcaactt cgcgatggat tgttcgaggc caaaatgaaa aacggcgccg
     3481 agcagccgtt ttggttttat ggttacggac atttcgggca ggtcatccgc gatttgccga
     3541 acttccgagg acttggcgcg tcgctggttc aagacggtca agtcggccca agttccatga
     3601 acgcggacgg ctcgttcggc ggatccgcca tccaactgtt caaggacctc cggaccgcgc
     3661 gacgcaatgg aatgcgaatt gattggctgc tgtcgccgca ttatttcccc gattgggcct
     3721 tcgccaagtc gcccgatgcc cgcggcggtg gattgggatt catcggattt gacattgatc
     3781 atccgacaac gcgcgaagtc ctggaaaatt tcaccggcgc aatcacggaa aagatgaagg
     3841 acgacgacgc gctgttcacc gtttgcctga gcaacgaacc ggtctatgac aaaagcggcc
     3901 gcacaaaata tggccgcagc gaatttgatg cgtatctgga aaacatccac gggcgcatcg
     3961 ggcaactgaa cgtattatac gggacccgct acacaaattt ttccgaagtc gcgccgcccg
     4021 cgaacgggtt gaaagcgacg gtgaatgaaa atcgcgctta ctacgactgg gtccgtttca
     4081 accagcggca tttcgccgcg tggcacgcgt ggcttggctc gcttgtgaag aagcatcttc
     4141 cctcgacacc aacgcacgcg aagatcatgg ttttttattg cctcgaccgc gacaaattgc
     4201 atttcggtgt tgaccccgaa ttgttctgcg acgcgaccga ccttgcgggc tgcgatgcct
     4261 atgcgtttcc aacgggcgac aaaacttacg actggatggg ccaggaattt ttctatgatc
     4321 tgttgcactc gttcaaaaac cagccggtgt tcaacagtga aaaccatgtc attcccgacg
     4381 gcgcagcgcc agacgatgtt cctttggcaa ccgcccgcgc ccaattctgg cagggcggcc
     4441 tgcatcatca ggcggcgacg acctgctggg tttgggaaga ggcgattgac acctcgctga
     4501 gcggcagcat ttatttccgg cccgcgaaca tttacggcgc aggccgcgcg atgctcgaat
     4561 tgaaccggtt cgccgccgaa gccgccgcca tcaatcgcct gcaagcccgc attgcgctgc
     4621 tttactcgcc cgcatccatt ttttgggagg acgcctacaa gggaacgatt caatcctgct
     4681 atcgccaact caattttctc ggcgaaccgg ttgatttcat cagcgaaaaa gaatttgccg
     4741 aaggccggat gccgacgaac gaattcatca ttgccccggc tgcgacccat gtcaccgacg
     4801 caaccgcgaa aaacttgcgt cgctttgttg aaactggcgg acatctgctt ttggcgggtg
     4861 cgggcaattt tgcctttgac gaatatcacc gcgcgcggcc aacctcggaa attcccgctg
     4921 gacttcaatt gccgtccgtt gcgcaagagc aggctgccgc tgacgccctg cgcgaaatcc
     4981 tggccaacaa cggcatcgcc gtgggcaacg tcatcgacaa cgcgacggga aaaccggcat
     5041 ggggcatcga gttccggcaa gcgaccgtga acggaaatcc ggtgatcgcc atgattgcat
     5101 tggatggcca aaatcattcc gcaagcatcc cggcattgca cggcaaaaaa gtcgttgatg
     5161 tcctcgcgaa cgaattgatt gaccccgaca aaatcaacct ggaaccgatg acgccgcact
     5221 tgttggaaat gcggtgacgc aacctcgaac acatctcata aataatggag cgcggctgtg
     5281 cgcgaagcgc cagccgcagc aacctgccaa aacattgagt gttccaagct gctggactgg
     5341 tactgcggac acagtcgctc cgttccctcg aaaagggtgc gtctccaaac ctcattgtcg
     5401 ggtccgactg tcccgttcct tcaacaaccg cttttcaaaa agcttttcgc cgcgcaccac
     5461 gccgaggatg tacaattttt tcgcgcgacg ttcgtaccga tggaaaatgc ggcagggaga
     5521 ctcgacgagc tggcggtaaa gagaatttcg cgagagttcg ggaatgcgag gaccgagttc
     5581 gggatgtttg gccagcaatt caacccgttc gaacacgcga cgcacaaggc gggaggcggc
     5641 gtccggcttg tccagcgcga tgtaatccgc gatgccgtca agatcgccca gcgctggttc
     5701 cgtccagatt atttcagcca tcgcgccatc cgctttttgg cggcttcatg ggaaaggatg
     5761 cggcccgttt caatcgcgcg ctcccctcga ctgatggttt caagaacggc aagcttttcg
     5821 ttcataccct cgaacgtctc cacatccacg acgtaggccg caggccgccc atgctgggtg
     5881 atgagcacgg ggtcgcggct cttcgccacc gaagcgatga tgtcggtcgc cttgcgcttg
     5941 agtgtcgtga cgagttcaat cttcataagt gatactctag tgacacatat ttggaatggc
     6001 aagggctttt aagtttcagg aaacg
//
LOCUS       DASZMM010000107         5018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3604979, whole genome shotgun sequence.
ACCESSION   DASZMM010000107 DASZMM010000000
VERSION     DASZMM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3604979"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VG754_02690"
     CDS             <1..1483
                     /locus_tag="VG754_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18140.1"
                     /translation="DRLFKSFSQADASTTRQYGGTGLGLAISKRLVELMGGRMWVESV
                     PQKGSTFHFILPFQPSQATARANLHGPLPQLADLKLLIVDDNPTNRRILTLQASKWGM
                     IPRSAQSGREALEWLQNGEAFDLAILDMQMPDMDGLMLAREIRQMPGAFMLPLVLLTS
                     MGIRSDNPAFANIAFASCLTKPIKPSQLQEALVRVVSGAKPAARKVAPAKLDPSLANR
                     LPLRVLICDDNLINQKVALRLLQQMGYKADVANNGVECLVALEQQPYDLIFMDVQMPE
                     MDGLEATRQIRQRQKSPGDFVHFKNNIVIVAMTANAMQGDREKCIASGMDDYIAKPVR
                     PEGLRVLLERWGTRATLSEPAETPATSAGHASPAPPISGESAFIDMDRLLDFANGDRE
                     NLRELIELYLQQTAKQLTQLAAAAESRKGDDLRRVAHSCAGASSTCGMVGITPMLREL
                     ERQGLEGIEPNAPQLAADAHREYERIKSFLTDYLKSSEQLAQT"
     gene            1499..2704
                     /locus_tag="VG754_02695"
     CDS             1499..2704
                     /locus_tag="VG754_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18141.1"
                     /translation="MKKILIIEDDQLVANIYRNKFAAENYKVEVALDGESGFENLNSF
                     HPDVIILDLMLPKMSGLDLLTKIRAQDKWKQLPVIVFSNTFLSNMVQQAWAAGATKCL
                     SKASCTPRQVIEVVRSLLPQSADSASDEDEGIGSGKPHSRHAKTAPLSDSDAALQAEL
                     RKVFLADLSPTIAACRANLQAIVKNAEETDRAKNLDQLYRRIHTLTGNAAIAGEPDIA
                     QLADALEALLRELCEKPKTINASILRTLAMTLDFASVLFERASQPQSQAASPAPKFNI
                     LVVDDEAISRRAVTYALEKAKLTCTAVENPEAALKLLAENGYDLVFLDVDMPGMNGFE
                     LCTKLRTFARNKTTPVVFVTSLTDFESRANSTMSGGNDLIAKPFLFMELAVKALIYVL
                     RGKIARASA"
     gene            2784..3233
                     /locus_tag="VG754_02700"
     CDS             2784..3233
                     /locus_tag="VG754_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18142.1"
                     /translation="MDPHSTVLIIEDTPDDVFILKMALQREGIINPVQVVTGGQEAID
                     YLCAKEPYNDRSKFPFPKVLFTDLKMPRIGGFDVLQWLRDHPDCSVIPVIVLSASRLD
                     EDVKRAYEMGANAYLVKPNNLEDLQRMVKTAFEFWQWCEVPQVAGKG"
     gene            complement(3253..3534)
                     /locus_tag="VG754_02705"
     CDS             complement(3253..3534)
                     /locus_tag="VG754_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HWF18143.1"
                     /translation="MCALYPAVRGNKPVTFILITPAVLAVLAITTWLGYGYVADGLHD
                     PDFEFGNDFLMPWILLAGAPMGTSLAAVFVLWFRRKKRSEAQLACDSMQ"
     gene            complement(3632..3910)
                     /locus_tag="VG754_02710"
     CDS             complement(3632..3910)
                     /locus_tag="VG754_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18144.1"
                     /translation="MKKYSKWPDFLFTLTVRFICGFILGGAACILVTFKGIMRAFANN
                     HLLTPVIWVCVFGIAGGLVAVFTIPAWQRPWYKGIREQRRDELLNRRR"
     gene            complement(4194..4661)
                     /locus_tag="VG754_02715"
     CDS             complement(4194..4661)
                     /locus_tag="VG754_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18145.1"
                     /translation="MREALVILVAEDNEHDVLLMKRALQKAAISDNVHFVSDGQDTID
                     YLAGNGKYADRNSHPLPHFAFLDIKMPRKTGLEALEWMRAQTRFKQLPVMVVSAAIEE
                     KDIKRAHELRASSYVVKPASVEQLVREFKRFYVFWWPIASSQKFVMGEYHAHA"
BASE COUNT         1183 a         1498 c         1240 g         1097 t
ORIGIN      
        1 cgaccggctt ttcaaatctt tcagccaggc ggatgcttcc accacgcgtc aatatggcgg
       61 caccggcctc ggtctggcca tcagcaagcg gctcgtcgaa ttgatgggcg gacggatgtg
      121 ggtcgaaagc gttccgcaaa aaggctccac gttccatttc atcctgccgt tccagcccag
      181 ccaggcaacc gctcgcgcga atctccatgg tcccttgccg caattggccg atttgaaatt
      241 gctgatcgtt gacgacaatc caaccaaccg ccgcatcctc actttgcagg cgagcaaatg
      301 ggggatgatt ccgcgcagtg cgcaaagcgg ccgggaagcc ttggaatggc tgcaaaatgg
      361 cgaagcattc gatctcgcca tcctcgacat gcagatgcct gacatggacg gactcatgct
      421 cgccagggaa atccgccaga tgcccggcgc gtttatgctg ccattggtgc tgctgacttc
      481 gatgggcatt cgctctgaca acccggcttt tgccaacatc gcattcgcca gttgcctgac
      541 aaagccgatc aagccatcgc aattgcagga agcgctcgtc cgagttgttt ccggcgcgaa
      601 acccgccgca agaaaagttg cgcccgcgaa actcgatcct tcgctggcca atcggcttcc
      661 gttgcgcgtg ctcatttgcg acgacaatct catcaaccaa aaagtcgccc tgcgtttgct
      721 gcaacagatg ggttacaagg ccgacgtcgc gaacaacggc gtcgaatgcc ttgtcgcgct
      781 tgagcagcaa ccctacgacc tcattttcat ggacgtgcag atgccggaga tggatggtct
      841 tgaagcgaca cgccaaatcc gtcaacgcca aaaaagtccc ggtgatttcg ttcatttcaa
      901 aaacaacatt gtcatcgttg cgatgaccgc caatgccatg cagggcgacc gcgaaaaatg
      961 catcgcctcc gggatggatg attacatcgc caaacctgtc cggcccgaag gccttcgcgt
     1021 tctgctcgaa agatggggca cgcgcgccac attgtcggaa ccggcggaaa cgccggcgac
     1081 gtccgcgggt catgcttctc ccgctccacc gatttcgggt gagtcagctt tcatagatat
     1141 ggatcggctg ctcgattttg ccaatggcga cagggaaaac ttgcgcgaac tgattgagct
     1201 ttacttgcag caaaccgcca agcagctaac ccaactcgcc gccgccgccg aatcgcgcaa
     1261 gggtgacgat ctcagacggg tggcccacag ttgcgccggc gccagctcca cttgcgggat
     1321 ggtcggcatc actcccatgc tccgcgaact tgagcgccag ggtcttgaag gcatcgagcc
     1381 caacgcgcca caacttgccg cggatgccca tcgtgaatac gagcgcatca aaagttttct
     1441 cactgattat ctcaaatcga gcgaacaact cgcccagact taacgaccag acgcgcgcat
     1501 gaagaaaatc ctcatcatcg aagacgatca actggttgcc aacatctacc ggaacaagtt
     1561 tgccgcggag aattacaagg tcgaagtcgc gctcgacggt gaatcgggct ttgaaaacct
     1621 caattcgttt catccggacg tcatcattct ggatctcatg cttcccaaaa tgtccggctt
     1681 ggatttgctg acgaaaatcc gcgcgcaaga caaatggaag cagctcccgg tcatcgtttt
     1741 ttcaaacaca tttctcagca acatggtgca gcaagcctgg gcggcaggcg cgaccaaatg
     1801 cctttccaaa gccagttgca cgccccggca ggtcatagaa gtcgtccgaa gcctcctccc
     1861 tcaatccgcc gactctgcat cagacgagga cgagggcatt ggttccggca agccccattc
     1921 ccgccatgca aaaacggcgc cgctatccga ctccgatgcc gcgcttcaag ccgaactgcg
     1981 caaggtcttt cttgccgact tgtcgccgac gattgccgct tgccgcgcca atttgcaagc
     2041 catcgtcaag aatgcggagg aaaccgaccg cgccaagaat cttgatcaat tataccgccg
     2101 catccatacg ttgaccggca acgcagccat cgcgggcgaa ccggacatcg cgcaattggc
     2161 ggatgcgctg gaagcgttgc ttcgggagct ttgcgaaaaa cccaagacca tcaacgcatc
     2221 catacttcga acattggcga tgacgctgga cttcgccagc gtgctttttg agcgcgcttc
     2281 gcagccgcaa tctcaagccg catcccccgc gcccaagttc aacatcctgg tcgtcgacga
     2341 cgaagccatc tcgcggcgcg cggtgactta cgcgctcgaa aaagcaaagc tcacctgcac
     2401 cgctgttgaa aatcccgaag ccgccttgaa actgcttgcg gaaaacggct acgatctcgt
     2461 ctttctcgac gttgacatgc ccggcatgaa cggctttgaa ctttgcacca agttgcgcac
     2521 gttcgcccgc aacaaaacca ccccggtcgt cttcgtgacg agcctgaccg attttgaaag
     2581 ccgcgccaat tcgaccatga gcggcggcaa tgacctgatc gccaagccat ttctcttcat
     2641 ggaacttgcc gtcaaggccc tgatatacgt cttgcgtgga aaaatcgcca gagcatcggc
     2701 ctgattcctt cgttttagaa aattttttcc tgcttccgct tcaacaggtt gaacgagcct
     2761 tcatcctgtg cgaggtttat tccatggatc ctcattccac ggtccttata attgaagata
     2821 ctcctgacga tgtgttcatc ttaaaaatgg cgttgcagcg cgaaggcatc atcaatcctg
     2881 ttcaagtcgt caccggtggg caggaagcca tagattatct ctgcgcaaag gaaccctaca
     2941 acgatcgctc caaatttcca tttcccaagg tgctgttcac tgacctcaag atgccgcgca
     3001 tcgggggctt cgatgtcttg caatggctgc gcgatcatcc cgattgttcc gtcatacccg
     3061 tcatcgtcct gagcgcttcg aggctggatg aagacgtcaa gcgggcgtat gaaatgggcg
     3121 cgaacgccta tctcgtcaaa ccgaacaatc tcgaggactt gcagcggatg gtgaaaaccg
     3181 cgtttgaatt ctggcaatgg tgcgaagtgc ctcaggtcgc gggaaagggc tgaacgggac
     3241 tcatcccgtt ttctactgca tggaatcaca ggccaactga gcttcactcc tcttttttct
     3301 cctgaaccat aaaacaaaaa cagccgccaa acttgtcccc attggcgctc ctgccaaaag
     3361 aatccaaggc atcagaaagt cattgccgaa ctcaaaatcg ggatcgtgca gtccatctgc
     3421 gacatacccg tatcccagcc aggtcgtgat cgccagaacg gcgagaacag caggcgtgat
     3481 caaaataaaa gtcaccggct tgttgccacg cacagccgga taaagcgcgc acaaagccag
     3541 cagggacgcc gccaatcctc cgcaaagaat cagtatcgcg aaaatgcctc catcaatgct
     3601 gtcggctaac attacgtgca tataattcca gtcatcgtcg cctgttcaaa agctcgtccc
     3661 ggcgttgctc gcggattcct ttataccatg gcctttgcca cgccgggatg gtgaacactg
     3721 ccaccaaacc gcctgcaatt ccaaatacac aaacccatat aacgggagtg agcagatggt
     3781 tgttggcgaa cgcccgcatt atgcccttga aagtcacaag aatgcaagcc gcgccaccca
     3841 aaatgaagcc gcagataaaa cgcactgtca gcgtgaaaag aaaatcaggc cacttgctgt
     3901 actttttcat ggctcgtctg tggaccgcga ctgtgtgcga agcgcccacc gcagcaagct
     3961 aaacaacccc tgaatattca agctcctgcg gacacagccg cgctccgttt ttatttatca
     4021 gatgacttgt aacactccca aaatggtttc tcaagccatt ccgcgccgca cttctctaat
     4081 agcccgttta ataactccag ttgtcgtggg agacgaggtg acgagtctca agtttatccc
     4141 attctgacga taagttagag actcgtcgcc tcgtgcctgc ttttttccgg ccgctacgcg
     4201 tgcgcgtgat attcgcccat gacgaatttc tgcgaactcg cgatcggcca ccagaaaacg
     4261 taaaatcttt tgaactccct caccagttgc tccacggaag cgggtttgac cacataagag
     4321 cttgcccgca attcgtgcgc gcgcttgatg tccttttctt cgatggccgc gctcaccacc
     4381 atcaccggga gctgtttgaa tcgtgtttgc gcccgcatcc attcaagcgc ttccaagccg
     4441 gtctttcgcg gcatcttgat gtcgagaaat gcgaagtgcg gcaaaggatg ggaatttcga
     4501 tctgcgtatt tgccattgcc cgccagataa tcaattgtgt cctgaccatc gctcacaaaa
     4561 tggacattgt cggaaatcgc cgctttttga agcgcccgtt tcatcagcaa aacatcgtgt
     4621 tcgttgtctt ccgccaccaa tatgaccaaa gcctctctca taatcccaat ccattactac
     4681 ccgcgtcgcc ggagatgcac aatccgtgcc aatcaggcac ttcttacttc ggaatatgga
     4741 gaatagataa tgttccaatc gctgccagtc aaattttgga ttgccggagt tactgggcgc
     4801 atccgcgcat tggcccggag cgcgggtcgg tgacccgcag caatgtggga aggataggcg
     4861 cgcatgaatt agtccggcat gtcctgtttg tcgcgcgttg ctgcggctca tcgagccgcg
     4921 ctcccctatt tagattttaa aaaacgcact tccttcattc ctgacgcata agtcgccgat
     4981 ttcttagaag aaaatcggaa ttgctggcat ggcaagcc
//
LOCUS       DASZMM010000108         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2792864, whole genome shotgun sequence.
ACCESSION   DASZMM010000108 DASZMM010000000
VERSION     DASZMM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2792864"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VG754_02720"
     CDS             complement(<1..894)
                     /locus_tag="VG754_02720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF18146.1"
                     /translation="MKRLRILHLNTQLNGGGTDDQCVKLAFALHQSGQDVKIAGPDGR
                     EFSKIIRALELPFHPTPPEGPLKLQFIRDVAKLIRRERTQIVHGHHGRDIWPTILAAK
                     LSGAKPKVVLTRHMAKSPGTWLSRRFMLGQCDALIAVSAFVKRVLCEGVYEPDSPEAE
                     RHARPPISGNFSKIHVIHGGIDTERFRPMDAEAQRREWGLQPDDHAFAVVGGYTLPRG
                     KGQREFLQAAARIHEKFPRAKFLIVGRGDMGDVLRADIERLGLKGKAWLTPYCLDMPR
                     AMNAIDCLVHSQVGTEAFGLVL"
     gene            complement(924..1706)
                     /locus_tag="VG754_02725"
     CDS             complement(924..1706)
                     /locus_tag="VG754_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF18147.1"
                     /translation="MSAPKISLIISTYQSPAALEKVLQGVLVQTQRPHEVLIADDGSG
                     APTRELVEGWQSRLPLRHIWHEDQGFRKTIILNQAVAAATGEYLVLLDGDCVPHAEFI
                     RDHGALAEKNYWVQGRRCFVQEKFVPEFMPGKIRVGSWCLSGRITGVAKAFRLPFPMV
                     RRNRKHRGIIGCNMAFWREDLIAVNGFDEEYTGWGGEDSDLGARLYHLGRTRKFVYGW
                     AVIYHLNHPQLPKAHVSASLEILRRTIASGKTRCQRGLEQYL"
     gene            complement(1682..2479)
                     /locus_tag="VG754_02730"
     CDS             complement(1682..2479)
                     /locus_tag="VG754_02730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF18148.1"
                     /translation="MNKLPITVSMISGAEAARIGRALDSVAGWTSEIVVVLNEEVRDG
                     TEEIAKRHGAIVIREKWHGFREQKNISLRHATEPWVLCLDADEEVSPELRESIFQFFN
                     GEGCKKHSGAYFARKVWFLGRWITHGDWYPDHVLRLIKKGSGKWEGSPEHCNIELDGP
                     VKKLRGDLHHYTNPTINSYVQKINYFSDIFLQRQLEAKKSWSATAVIARSAWRFLRGY
                     VFRLGFLDGFPGFFIAASTAYSTLVRHSRLYEHLHSDAPPCPPRKFP"
     gene            2675..2998
                     /locus_tag="VG754_02735"
     CDS             2675..2998
                     /locus_tag="VG754_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HWF18149.1"
                     /translation="MHHSHSHTVNNHHHNPYSARKMAKPINFICVAPAAQHVHLIGDF
                     NDWDPAAYPMKRQPDGAWLIQIPLTHGHHHYQFLVDGKPTLDPRAQGIARNENNEKVS
                     LLAVS"
     gene            complement(3287..>3790)
                     /gene="tadA"
                     /locus_tag="VG754_02740"
     CDS             complement(3287..>3790)
                     /gene="tadA"
                     /locus_tag="VG754_02740"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HWF18150.1"
                     /translation="IDLQSDQYFMGEALRQAAKAYEAEEVPIGAVVVRGGRIIARAFN
                     QVELLKDATAHAEMLALTQAEETVGDWRLTDCTLYVTKEPCPMCAGAIVHVRLARVVF
                     GAGDPKAGAAGGALNLLQFPSFNHQCEITRGVREADCRALLQTFFAEQRAKGKQAPSN
                     GEGALES"
BASE COUNT          813 a         1144 c          988 g          845 t
ORIGIN      
        1 cagcaccagt ccgaacgcct cggtgccgac ctgcgaatgc acgaggcaat caatcgcgtt
       61 catcgcgcgc ggcatgtcga ggcaatacgg cgtcagccat gccttgccct tcaagccaag
      121 ccgttcgatg tccgcgcgca atacatcgcc catgtcgccg cggccgacga tcaagaattt
      181 cgcgcgcgga aatttttcat ggattcgcgc cgccgcttgc aaaaattcgc gttgtccttt
      241 tccgcggggc aaagtgtaac cgccaacgac tgcgaaagca tgatcgtccg gctgcaaacc
      301 ccactcgcgc cgttgcgctt cggcatccat gggacgaaat cgttccgtgt ctatgccgcc
      361 atggatgacg tgaatcttgg aaaaattacc ggaaatcggc ggacgcgcgt gccgttccgc
      421 ttccggcgaa tcgggttcgt aaacgccttc gcacaaaacc cgtttcacaa atgcggacac
      481 ggcgatgagc gcatcacatt gtccgagcat gaagcgacgg ctcaaccatg tgccgggact
      541 tttggccatg tgccgcgtga gcaccacttt tggttttgca ccggacagtt tcgcggcaag
      601 aatcgtcggc cagatgtcgc gaccgtgatg gccatgcacg atctgggtgc gttcgcggcg
      661 aatcaatttc gccacgtccc ggatgaattg caatttcaac ggcccttccg gcggcgtggg
      721 gtgaaaagga agttcgagcg cgcggatgat tttggaaaac tcgcgtccgt caggcccggc
      781 aatcttgacg tcctgtcccg attgatgcag ggcgaaagcc agtttgacgc attgatcatc
      841 cgtcccgccg ccgttcaact gcgtgttgag atgcaggatg cgcaggcgct tcattggctc
      901 cccgggcggc gctgtttcat ggttcaaaga tattgttcca gcccacgttg gcaacgggtt
      961 ttgccggatg cgattgtccg ccgcaagatt tccaaacttg ccgagacatg cgccttgggc
     1021 agttgtggat ggttcaaatg atagatgacc gcccagccat aaacaaattt gcgcgttcgg
     1081 cccagatgat aaagccgcgc gcccaaatcc gaatcttcgc cgccccagcc ggtgtattct
     1141 tcatcgaagc cattgaccgc gatcaaatcc tcgcgccaga atgccatatt gcagccgatg
     1201 atgccgcgat gtttgcggtt gcgccgcacc atcgggaacg gcagccgaaa ggccttcgcc
     1261 accccggtta tccgtccgct caaacaccag cttccaacgc gtatttttcc cggcataaat
     1321 tccggcacga atttttcctg cacaaaacaa cgccgtccct gcacccaata atttttctcc
     1381 gccaatgcgc cgtggtcgcg gatgaattcc gcatgcggca cacaatcgcc atcgagcagc
     1441 acaagatatt cgccggtcgc cgcggcaacc gcttggttca ggatgatggt tttgcggaag
     1501 ccctggtctt cgtgccagat gtggcgcaat ggaaggcgcg attgccagcc ttctacgagt
     1561 tcgcgcgtcg gcgcgcccga accgtcgtcg gcgatgagca cctcatgcgg acgttgcgtt
     1621 tgcacaagga ctccttgcaa cactttttca agcgccgccg ggctttgata ggtgctgatg
     1681 atcagggaaa ttttcggggc ggacatggcg gagcatccga atggagatgt tcgtaaagcc
     1741 ggctgtgccg gacaagggtc gagtaggccg tcgaggcagc gatgaaaaat ccgggaaatc
     1801 cgtcgagaaa gccgagccgg aaaacataac cgcgcaaaaa tcgccaggcg gaacgcgcaa
     1861 tgaccgcggt ggcggaccag cttttcttcg cctcaagctg gcgttgcagg aaaatgtccg
     1921 aaaaataatt tatcttctgc acgtaactgt tgatcgtggg gttcgtgtaa tgatgcaaat
     1981 cgccgcgaag ctttttcacg ggtccgtcaa gctcgatgtt gcaatgttcc gggctgcctt
     2041 cccacttgcc ggaacctttt ttgatcaatc gcaaaacatg gtcgggatac cagtcgccgt
     2101 gcgtgatcca gcggcccagg aaccaaacct tccgggcaaa atacgctccc gaatgttttt
     2161 tgcaaccctc gccattgaaa aattgaaaga tggattcgcg caattccggc gaaacttcct
     2221 cgtcggcgtc caggcacaac acccacggtt cggtcgcgtg gcgcaatgaa atatttttct
     2281 gctcacgaaa accgtgccat ttttcgcgga tgacaattgc cccgtggcgc ttggcgattt
     2341 cttccgtccc gtcgcgcact tcctcgttca gcaccacgac gatttcgctc gtccagcccg
     2401 ccacgctgtc caaggcgcgc ccgatccggg cggcttccgc cccggaaatc atgctgacgg
     2461 tgatgggcag cttgttcact ggctgattgt ggacgcgctg atgcgccgtg ataatggaag
     2521 caagtcaatc atccccccgc cggactgaaa agccaaaaac tttttgcgtt ttgtcttgcg
     2581 cgccgcattt tccgtagtaa ttttctccgc catacgttcg cgggcgcgtg gagtgccgcg
     2641 ggccgttttg aaatcagcaa cacgttgtaa ccagatgcat cattcacatt cgcacactgt
     2701 caataatcac catcacaatc cctattccgc caggaagatg gccaaaccga tcaacttcat
     2761 ctgcgtcgct ccagccgcgc agcatgtcca tcttatcggc gatttcaatg attgggaccc
     2821 ggccgcttac ccgatgaaac gccaacccga cggcgcgtgg ctcatccaaa tcccgctcac
     2881 ccatggccat catcattacc aattcctcgt ggacggcaag ccgacgctcg atccccgcgc
     2941 ccaaggcatc gcccgcaatg agaataacga gaaggtttcg ctgcttgcgg tgagctgatt
     3001 cgttgggtgg ttgaaagatt aaatcattac agggccattc cgtcggaaaa tagaattagg
     3061 aaagcaggaa atcaggaaaa gacgacgttt cctcaggatt ttgttcctga tttcatgatt
     3121 tcctgataaa aaactcttaa tctcattttc tgccgttcat ctggtttttg ccaataaaat
     3181 cgcaggtccc gactggcttg accggctatg ggatgctacc gaaatcgtta catcgttaca
     3241 aacgcgcaca atcctgcaat tggtgacctt gcaaccattt aatcttttaa ctttccagcg
     3301 caccttcacc gttgctcggc gcttgctttc cctttgcgcg ctgttcggca aagaaagttt
     3361 gcaacaacgc ccggcagtcc gcttccctca cgccgcgcgt gatttcgcat tgatgattga
     3421 atgagggaaa ctgcagcagg ttcaacgctc cgcctgccgc gcccgccttc ggatcgcccg
     3481 ctccaaaaac cacccgtgcc aaacgcacat gcacaatggc gcccgcgcac atcggacacg
     3541 gctccttggt cacgtaaagt gtgcaatccg tcaaccgcca gtcaccgacc gtttcctccg
     3601 cctgcgtcaa cgccagcatc tccgcgtggg cggtcgcgtc cttgagcaat tcgacctggt
     3661 tgaacgcgcg cgcaatgatc cgtccgccgc gcaccaccac cgcgccgatt ggcacttcct
     3721 cggcttcata agcctttgcc gcctgacgca acgcttcgcc catgaaatat tggtcgcttt
     3781 gcaggtctat
//
LOCUS       DASZMM010000109         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_591131, whole genome shotgun sequence.
ACCESSION   DASZMM010000109 DASZMM010000000
VERSION     DASZMM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591131"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VG754_02745"
     CDS             complement(<1..1327)
                     /locus_tag="VG754_02745"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18151.1"
                     /translation="MSQSLSAHSKAAPMPLLLLLRVNALLTWRRLKSVRDQSWLLTAL
                     ILCFIVGYLGLSFWLFFKGLKFIGAFPGLGTVLTERLLYLLFAFLFALLLLSNLIISY
                     TNLFRNREAGFLMSMPIPAQTIFQWKFFESTLLASWAFLFLIAPLLVAFGLTHDVAWH
                     FYPMTLLLIALFIFLPALAGSWLALNLARYLDRQAFQISALIVALGSLVCAVIWWHHK
                     PPNDDMMETRVLAVLDKLLQQTKFSQFPFLPSYWLSTSVLQWSQGALELAGFFALVLL
                     SNVLFFGFLAFTRLGNSYYDAAARVQSRESVWGQWEWFRERARRGVPFTYPIGPAEKL
                     INLLRWIAPDTRALLVKDVRMFWRDTTQWGQTVLLFGLLGVYVVNVRHFTSQLVNPFW
                     IHLVSYLNLGACSLNLATLTTRFVYPQFSLEGRRLWIVGMAPMGLPKVVR"
     gene            1984..3534
                     /locus_tag="VG754_02750"
     CDS             1984..3534
                     /locus_tag="VG754_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18152.1"
                     /translation="MTAKSSLPFAQKLFFAICLLMGVRSASAFALLGPFAPWMTTQLS
                     YQDGVSIGGPMNIGEGYRWNVPVITYGYDQSFLDFFGTNGVAAVESAIQILNNLPPAS
                     QIILTNYPLDSLRVNYHAQAQNLIDLKSSALSSLVEHLGLAQPTRYVYCLHDFTFSNG
                     VPIYTVLNRNFDPMTWAPSTNVNGVVYDDGITVNESWEVYGSNVFQRNTYLPLFPILF
                     VDAQEIAVDPNESSEVDDAVADNASGSYYGSIFLNLTQDDAGGLAYLLSSNNTALESL
                     IPGVQGTGTNTTNFVNTALRPGVEKITFQRTDYISPSQHTFPVVTNQYVDSYYTNGVL
                     QQQTLQRVITQPDILFTANYIKLQNVSRTGTTNWINNGTPGNDGPGVIQPSSVINFNQ
                     LGPSLYIGSPNYDTNAAGAVLPVWGSFDSSTNPPVAYPIVSNANCTRFHFILQGLPSN
                     PFASIPQTDYAWNLTGQPNALFSLQTSTNLQNWLTIGTITNLGGTFTYVDWTWTNTPQ
                     RFFRTVPQ"
BASE COUNT         1074 a         1206 c         1043 g          915 t
ORIGIN      
        1 ctctcacgac cttcggcaaa cccatcggcg ccatgccgac gatccacaat cgccgtcctt
       61 ccaacgaaaa ttgcggataa acaaatcggg tcgtgagtgt cgcaaggttc agcgagcacg
      121 cgcccaaatt gaggtacgaa accaggtgta tccagaacgg attcacaagc tggctggtga
      181 aatgcctcac attcaccaca tacacgccca gcaagccgaa caacaaaacc gtctggcccc
      241 attgcgtcgt gtcgcgccaa aacatgcgca catctttgac gagcaacgcc cgcgtgtccg
      301 gagcgatcca ccgcaacaga ttgatcaatt tttccgccgg gccaatcgga taggtaaaag
      361 gcacgccgcg ccgcgcccgt tcccggaacc attcccattg cccccagaca ctctcgcggc
      421 tctgcacccg cgccgccgcg tcgtaatacg aattgcccaa tcgcgtgaat gcaaggaaac
      481 cgaaaaacaa aacgttgctc aacagcacca gcgcgaaaaa tccggccagt tccagcgcgc
      541 cctgcgacca ttgcaacacg ctcgttgaaa gccagtagct cggcagaaat ggaaactgcg
      601 agaatttcgt ttgttgcagc agcttgtcca gcacggcgag cacgcgcgtc tccatcatgt
      661 catcgtttgg aggcttgtga tgccaccaga tgaccgcgca aacaagcgaa ccgagcgcca
      721 cgatcaacgc gctgatctgg aacgcctggc ggtccaggta tcgcgcgaga ttcaatgcca
      781 gccacgaccc cgccaacgcc ggaagaaaaa tgaaaagcgc gatcaacagc agcgtcatcg
      841 gataaaaatg ccaggcgacg tcatgcgtca atccgaacgc caccagcaac ggcgcgatca
      901 aaaataaaaa cgcccaggac gcgagcaaag tggattcaaa aaatttccat tggaaaatcg
      961 tctgcgccgg gatcggcatc gacatcagga aacccgcctc gcgattccgg aacaggttcg
     1021 tgtaactgat gatgagattg ctcagcagca acagcgcgaa caggaacgcg aacagcagat
     1081 acaacagccg ttctgtaagc accgttccca aacccggaaa tgcgccgata aatttcagcc
     1141 ccttgaaaaa cagccagaac gaaagcccca gataaccgac gatgaagcag agaatcagcg
     1201 ccgtcagcaa ccacgactgg tcgcggaccg atttcaagcg ccgccacgtc agcaacgcgt
     1261 tgacgcgcaa aagcagcaac aacggcatcg gcgccgcctt ggaatgcgca gacaaactct
     1321 gagacattgc cgcactctag cagaatcgtt tgcgtccggc aatgctggag gaagagcctg
     1381 aatggttctc gcgaaggccg cgaagaacgc gaagtttttc gaaagttgca ctcatgcttg
     1441 ctaggtaggg gccctcgtgg gagcgcgcgc ctccggcgtg cagtgttcgg cgtcccgccg
     1501 aacacttcgt aatactgatt ttctgtggtg tcaatgcaaa ggctttctgc caagtacagg
     1561 ctgatttgca gcatttcctg ttgcagcagc gccgtaggcg cggcctcttt gtagaaatcc
     1621 atcaacaaaa caaatccagc cccgttagcg ggcggcatat tccgacgatg tcgctcctga
     1681 cggagctttg gcttttaatt ttattttttc tacaaatagg ccagccctaa cgggctttaa
     1741 atcattccat cgcgggaatg ttccgaacac cgggaaatgg tgaaagccct ctccttgggt
     1801 gagagggttt gggtgagggc gagcgtcctc caaaccgcac ctgtgcattc tcaataacag
     1861 tggcaagatg cacccacacc accattttcc cagcctttcg gcttgtgttt cacagaagat
     1921 ttagcgaaga ttgaagttcc ttgcacagaa aagatgttcc aaggagaaat acaaatattt
     1981 gatatgactg cgaagagttc cctgccgttt gcacaaaagc ttttttttgc gatttgcctt
     2041 ttaatgggtg tccgttccgc atcggcgttt gccttgctcg gcccgttcgc gccgtggatg
     2101 accactcaat tgtcctatca agacggagtt tccatcggcg gcccgatgaa catcggtgaa
     2161 ggataccgtt ggaacgttcc cgtcatcacc tacggctacg accaatcgtt tctcgatttt
     2221 ttcgggacca acggcgtcgc cgcggtcgaa agcgccatcc aaatcctgaa caatctgccg
     2281 cctgcttcgc aaattatttt gacgaattat cctctcgaca gcttgcgggt aaattatcat
     2341 gcccaagcgc agaatctgat tgacctgaaa tcctctgcgt tgagctcgct ggtggagcat
     2401 cttggtcttg cgcaaccgac ccggtatgtc tattgcctgc atgatttcac cttttcgaat
     2461 ggagtgccaa tctacacggt attgaacaga aattttgatc cgatgacttg ggcaccctcg
     2521 accaatgtga atggcgtcgt ttatgatgac gggatcaccg tcaacgaatc atgggaagtc
     2581 tatggctcaa atgtatttca gcgaaatacc tatcttccgc tttttcccat cctctttgtg
     2641 gatgcacagg aaattgcggt ggatcccaat gaaagcagtg aagttgacga tgccgttgca
     2701 gataatgcat caggtagcta ctacggtagc atttttctta atctgaccca agacgacgct
     2761 ggcggactgg cttatctcct gagttcaaat aacaccgcct tggaaagctt gattcctggc
     2821 gtgcagggca cgggcacgaa tacaaccaat ttcgtaaata ctgctttgcg tccgggtgtg
     2881 gagaaaatca catttcaacg aactgactac atttcccctt cacaacacac ttttccggtt
     2941 gtcacaaacc aatatgtgga ttcatattat acaaacgggg tgttgcagca acaaacatta
     3001 caacgggtca tcactcaacc cgatatttta ttcacggcca attatatcaa attgcaaaat
     3061 gtttccagga ccggaacaac caattggatc aacaacggaa ctcctggtaa tgacggcccc
     3121 ggcgtcatcc agccatcgag tgtgatcaat ttcaatcaac ttgggccgag tctgtatata
     3181 ggatcgccga actacgatac gaatgctgca ggagccgtgc tgccggtttg ggggtcattt
     3241 gattcgagca caaatccgcc ggttgcttat ccaatcgttt caaacgccaa ttgcacccgt
     3301 ttccatttta ttctccaagg cttgccctca aatccgtttg cctccattcc acaaactgac
     3361 tatgcttgga acctcacggg ccaaccgaac gcacttttct ccctgcagac ttcaaccaac
     3421 ttgcaaaatt ggctgaccat cggaaccata acgaaccttg gcggcacatt tacctatgtg
     3481 gattggactt ggactaacac cccccaacgt ttcttccgca ccgtaccaca gtaggcataa
     3541 gtcaaagcgg caggggaatg ccgcactccc aggacgcagc gccgtaggcg cggcctcttt
     3601 gtagaaatcc atcaacaaaa ccaatccagc cccgttagct ggcggcatat tccgacgatg
     3661 tcgctcctga cggagctttg gtttttgatt ttcttttttc tacaaatagg ccagccctaa
     3721 cgggctttaa aaataatgga ggaatggttc ccacgatgca ttcgtccgaa caggaacaac
     3781 cagatattag ccagatacgc agtgtctgat ctgcccacac gaccataggc gatctggaca
     3841 ctgtcccgga tttgtcggtc atgccggata gacgggttga gcttgccggg gccaccgggt
     3901 aaaacggagc gcggctcggt gagccgcagc aacgcaccag aaccaaaaca cgcccgacta
     3961 attcggacac agccggtatt cccacattgc tgggggtcat cgacccgcgc tccggggcag
     4021 tgcgccgatg cgcctcttgg cactgtcgag atttgtcggt catggcagat agacgggttg
     4081 agcttgccgg ggcgttcacc gggtaaaacg gagcgcggct cggtgagccg cagcaacgca
     4141 ccagaaccaa aacacgcacg actaattcgg acgcaaccgg tattcccaca ttgctgcggg
     4201 tcatcgaccc gcgctccggg gcagtgcgcc gatgcgcc
//
LOCUS       DASZMM010000110         8308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2793447, whole genome shotgun sequence.
ACCESSION   DASZMM010000110 DASZMM010000000
VERSION     DASZMM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793447"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VG754_02755"
     CDS             complement(<1..250)
                     /locus_tag="VG754_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18153.1"
                     /translation="MERAPGQWDWQQHDAMEHNLHAAGLKYVVYNWVHFPPAWLKEKR
                     TLMRCLEHSKETSYLSIFDPQTIEWYDHFYRNLHEHFGD"
     gene            785..2077
                     /locus_tag="VG754_02760"
     CDS             785..2077
                     /locus_tag="VG754_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18154.1"
                     /translation="MIRRVKKPATTGNRAAYPAPSEQAHTPGEQARQNYDAQSVALAE
                     LQTIYEHAPLGICLFDCDLRYIKINQRLANLSGRPVVEHVGRPLRELLPLLADVLEPI
                     LKSVVETCEPVKNLEMREPEPHPGCSARTARVSFHPVKDSEGRLIGINALVLDITERE
                     DALRDYAEELERRVAERTATLQESVQSLEGVLYHVAHDLRAPLRAMQGLTTLLLEEYA
                     TRFDDEGRDYAARIVTAASRMDALIRDLLAYGRLGHVDLPLQKVDLEKLMKFALDALC
                     DEVAERGAQIEIQHPLPPVHANELVLQQIVLNLLSNALKFVAPGVAPRVRIWPETSND
                     KIRLWMQDNGIGIGTEHQQRIFRVFERLHRSEDYPGTGIGLAIVYKGMERMGGSVGVQ
                     SQPGAGSRFWLEFPGRNEAAGTALPENSGPVKDAPLQK"
     gene            complement(2052..3461)
                     /locus_tag="VG754_02765"
     CDS             complement(2052..3461)
                     /locus_tag="VG754_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HWF18155.1"
                     /translation="MLERFFRLSENGTTARTELLAGMTTFLTMAYVIVVQPAVLSGAM
                     FGMKTGMDFGAVMTATCLSAALATAIMALYARYPIAQAPGMGQNFFFVFSAMPAAAAA
                     GFSNSWQVALGTVFISGMLFLILSLCGLREMILDSVSPSLKNGIAVGIGLFIAFIGLQ
                     NTGLIIKDPGTAVKMNAHFASPDLLVFFFGLLLTAALHARKVSGSILWGILGATATAI
                     VLKIALPRLPSIASSSLFAESMLNKRFEFAKGILAAPPSLSPTFLQMDLRGALSAAMW
                     PFIFVFLFMLVFDAIGTLIGVAELAGFIKDNKLPRARQAMLSDAVGTVAGAALGTSTV
                     TSFIESAAGVEQGGRTGLTGLATAGLFLLALFFSPVIIMLGSYPPITAPALVVVGAMM
                     SRNVIKIDWNDHSESVPAFLTMVGIPLSYSIADGMALGFISYPIIKFLSGRGSDVHWL
                     TYALALVLIAYFIFVRARL"
     gene            complement(3454..4401)
                     /locus_tag="VG754_02770"
     CDS             complement(3454..4401)
                     /locus_tag="VG754_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWF18156.1"
                     /translation="MNHIVFEALAKAARSGEPAALALISGAKGSSPQKVGAKALFYSD
                     GRIQGTLGGGCLEAEIQQRAIQALRTGEPATFDLLLDHDFGWDDGLICGGKVFGVILP
                     NAVKVGAEFWSQLAERKRQIVWGVRKDFDVKHSSATDMNHGNDWLYLETISPPCHLWI
                     AGAGHIAQAVAPLAAQLDFKATVFDDRPALANHHYFPDEIAIQTDEWEKLAALPLPDA
                     PSFALIVTRGHRHDALVLKDWIHKRFLFIGMIGSARKSRIIFDHFRDEQIATPEQLAR
                     VACPVGIKIKSRSVPEIAVSIMAQFIEKRAELVLESKHA"
     gene            complement(4398..8174)
                     /gene="xdhB"
                     /locus_tag="VG754_02775"
     CDS             complement(4398..8174)
                     /gene="xdhB"
                     /locus_tag="VG754_02775"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HWF18157.1"
                     /translation="MRGAIEFVLNGRAVRVRDCSPNLTLLEYLRGHGCTGSKEGCAEG
                     DCGACSVAIIDRDSNGHACYRAINSCLVPLPSLAGREVITVEGVAGGNGELHPVQRTM
                     VECHGSQCGYCTPGFIMSLFEGYYRKDLQNDSQLDAQLCGNLCRCTGYRSIHEAAVEA
                     FKQKNKQNGEDPFVAKLSHAFAEPSATHYEFSGEKFFRPASLDELLRLMQEHPEARLV
                     AGATDLGLEITKKYKKFPTLISVEATPELKELQSSPSEWRIGAAVTMTELEDALGAEY
                     PALADMLRVFGSRQIRNRATLGGNLVTASPIGDSAPVLLALDAKVVLASLQGERVLPL
                     EEFFIAYRKTALQPGEVLKTILMPRGASQSNLTRKCQWYKVSKRREMDISTVAACFTV
                     DLDAAGVVRYARLAYGGVAALPVRARKTEQALIGNKWSAELIAEMLPTLRTEFTPISD
                     VRGEARYRQELVKNLFEKFFYEKPDAAKQALKTHVFSYENSAHPLPHESAHKHVTGEA
                     IYVDDQTAGMLETWPVCSPYARAKILKRDATEARRMPGIKAVLLAEDIPGRNDVGAVI
                     HDEILLADREVFFHGHLVALVIGDSQEACRAAAAKVIVEYEPLTPILSLPQAVREGSF
                     HNEPNFIRRGDVVQGLQSAPFAFEGEFEFGGQEHFYLETNAAWAQRGEDGSMFVCSST
                     QHPSEVQNVISHVLHLPMNRVVVQSPRMGGGFGGKETQAATPAALAALAAHHTGRAVR
                     VRLNRDQDMMLLGHRHPFLARFQVGYDAQGMLLAVKAHLYSNGGWSLDLSQAVTDRAV
                     FHLDNSYYIPQVEFRGQVAKLNVSSNTAFRGFGGPQGMLVIEEIIDRVARRLGLTPEI
                     VRERNLYRGRGETNTTHYGQEIEDNRINVLWNQLKESSGIEKRREEIKSWNAQNPHRK
                     RGLAMTPVKFGISFTVTHLNQAGALVLLYQDGTAQVNHGGTEMGQGIHTNIRAIAAKE
                     LGVKNEDVRVMPTSTDKVPNTSATAASAGTDLNGAAVKNACEILRERLRPVAAGCLKE
                     KSARETSLENVLFVEGFVSDREHPEVRIPLAEIVRKAYFQRISLSATGYYRTPDIHWD
                     RAKGRGKPFHYFAYGAAVTEVEVDGFTGMMQVLRTDILQDVGDSINAGVNRGQVEGGF
                     VQGMGWLTTEELKWDDQGRLLTHSPDTYKIPSIGDRPQIFNTQFLQNAAQKNVIHGSK
                     AVGEPPLMLAISVREAIRDAVAAFGPPANEIPLASPATSEAIFMAIQKQKEKPA"
BASE COUNT         1899 a         2357 c         2256 g         1796 t
ORIGIN      
        1 ggtcgccaaa atgttcgtgc aggttgcgat agaaatgatc gtaccattcg atggtttgcg
       61 ggtcaaagat ggagagatag gaggtttcct tgctgtgttc aaggcagcgc atcaacgtgc
      121 gcttttcctt caaccaggcc ggtggaaaat gcacccaatt gtaaaccacg tatttcaacc
      181 cggcggcatg gagattgtgt tccatcgcat catgttgctg ccagtcccat tggccgggcg
      241 cgcgttccac agcgccccat gcgacgtaat cctcattcgc gaccagaccg aaatgttgaa
      301 gccgttcttg ggattcaacc gaaggcggcg cttccagata atttgttcgc ggaaaagccc
      361 cgattggaga tgcctgaatg aacgagactg acagattacc ggttgaaatg ttccaaggcc
      421 gcccgtcgaa cgcggcgttg gttagtccag gtaaagaacc tgccccgcaa tggaatgcga
      481 taggcagcag gatgaacagg aaggtccgcc tatggacgca ttgagagaca aattttttga
      541 ttcgcggagt tatgatgaag cgaggatact cagccagact catttcgtcc cgcgaaatcg
      601 tcaacgccgt agtggatttc agagaaccgc ctctacagac tgtgtccgaa aaattttgga
      661 tatgatacgg ggacagtacc tccttgctaa tgttgaaatc tgttggaagt tgaggggcaa
      721 acgacgatgg aattcggaaa tggcgttcgt cgaacgatcg aatgaaataa cagtttcatt
      781 ttttatgatt cgtcgagtta aaaaaccggc cacaactgga aatcgtgctg cctatccggc
      841 gccgtcggaa caggcgcata cgcccggtga acaggcccgc caaaattacg atgctcaatc
      901 cgtggcgctg gcggagttac aaaccatcta tgagcatgcg cctttaggca tctgtctctt
      961 tgattgcgac ctccgctaca tcaaaatcaa ccagcgtctg gcaaacctga gcggacgtcc
     1021 cgtcgttgag catgttggac ggcctttgcg ggagttattg cccttgctcg ccgacgtgct
     1081 cgaacccatt ttgaaaagtg ttgtggaaac ctgcgaaccg gtgaaaaact tggagatgcg
     1141 tgaaccggaa ccccaccccg ggtgctccgc gcgcactgcc agagtcagtt ttcatccggt
     1201 gaaagattcg gaaggacggc tcataggcat caatgcgctg gtgcttgaca tcacagagcg
     1261 tgaagatgcc ttgcgcgatt atgccgagga acttgaacgt cgggttgccg agcggacggc
     1321 cacgttgcag gaaagcgtcc aatcccttga aggtgtcctt taccatgtgg cgcatgattt
     1381 gcgcgcgccg cttcgcgcca tgcagggact taccacgctg ctgctcgaag aatatgcgac
     1441 tcgtttcgat gacgaaggac gcgattatgc cgcgcgaatt gtcaccgccg ccagtcgcat
     1501 ggacgcactt atccgtgatc tgctcgctta tggccgcctc gggcatgtgg acctgccgtt
     1561 gcaaaaggtg gatttggaaa aactgatgaa gttcgccctc gatgccttgt gcgatgaggt
     1621 cgcggaaagg ggcgctcaaa tcgaaataca gcatccgttg ccgccggttc atgccaatga
     1681 acttgtgctc cagcaaattg tccttaatct gctttccaac gccctgaaat tcgtcgctcc
     1741 cggtgttgca cctcgtgtcc gtatctggcc ggaaacttcg aacgacaaaa tccgcctttg
     1801 gatgcaagac aatggtattg gcatcggaac cgagcatcag caacggatct tccgcgtgtt
     1861 cgaacggctg catcgcagtg aggattaccc gggaactggc atcggcctgg ccattgttta
     1921 caagggaatg gaaagaatgg gcgggtctgt cggcgtccaa tcccaacctg gggccggcag
     1981 ccggttctgg cttgaatttc caggcaggaa cgaagctgct ggcactgcgc tccccgagaa
     2041 tagcgggccg gtcaaagacg cgcccttaca aaaataaaat aggcgatcaa caccagagcc
     2101 agcgcataag tcagccaatg cacatcactt ccacgtccgc tcaaaaattt tataatagga
     2161 tagctgatga accccaaagc cattccgtcg gcgattgaat acgaaagcgg aatcccaacc
     2221 atcgtcaaaa acgcgggcac cgattcacta tgatcgttcc aatcaatttt gatcacgttc
     2281 cggctcatca tcgctcctac aaccaccaaa gccggcgcgg tgatgggtgg atagctgccg
     2341 agcattatga tcaccgggct gaaaaagagc gccaacaaaa acaatcccgc cgtcgcaagt
     2401 cccgtcaaac ccgtccgtcc gccttgctcg acgcccgcgg cgctctcgat gaaacttgtg
     2461 accgtgctcg ttcccagcgc cgctcctgcg accgtgccca ctgcatcgga aagcatcgcc
     2521 tgccgtgcgc gcggcaattt gttgtccttg atgaatcccg cgagttcagc gacgccgatg
     2581 agcgtcccga tggcatcaaa caccagcatg aataaaaaaa cgaagatgaa cggccacatc
     2641 gccgccgata acgcgccgcg caaatccatc tgcaaaaatg ttggcgacaa cgacggcggg
     2701 gcggcgagaa tccctttcgc gaattcaaac cgcttgttta acatcgattc cgcgaaaagg
     2761 gacgagcttg cgattgaagg caagcgcggc aacgcgattt tcaacacgat tgccgttgct
     2821 gtcgcgccaa gaatgcccca aagaattgag ccgctcactt ttcttgcatg aagcgctgct
     2881 gtcagcagca atccgaagaa aaacaccagc aaatcgggtg atgcgaaatg cgcgttcatt
     2941 ttcactgcgg tccccgggtc tttgatgatc aacccggtgt tctgcaggcc gatgaatgcg
     3001 atgaacaaac cgattcccac cgcgatgcca tttttcaaac tggggcttac cgaatccaaa
     3061 atcatttcgc gcagcccgca cagcgacaag atcaaaaaaa gcatgcccga tatgaaaacc
     3121 gtcccgagcg ccacctgcca cgaatttgaa aaacctgctg cggctgccgc aggcatggcc
     3181 gagaacacaa agaaaaaatt ctgtcccata cccggcgctt gcgcgatggg ataacgcgca
     3241 tacagcgcca tgatggctgt ggccagcgcc gcggaaagac acgttgcagt catcaccgcg
     3301 ccaaaatcca tgccggtctt catcccgaac atcgcgccgg acaacaccgc cggctgcacg
     3361 acgatgacat acgccatcgt cagaaaggtc gtcattcccg caagcaattc cgttcgcgcg
     3421 gtggtaccgt tttccgagag cctgaaaaat cgttcaagca tgtttggatt cgagcacgag
     3481 ttcagcgcgt ttctcgatga attgagccat gatgctgaca gcgatttcgg gaacgcttcg
     3541 ggattttatc ttgatgccga cgggacaggc cacgcgggcg agctgttccg gcgtggctat
     3601 ttgttcgtcg cgaaaatggt cgaagataat ccgtgacttg cgcgcgctgc cgatcatccc
     3661 aataaacaaa aatcgtttgt gaatccaatc cttgagcaca agggcgtcat ggcgatgacc
     3721 gcgcgtaaca ataagcgcga atgacggcgc gtctggcaac ggcagcgccg ctaatttttc
     3781 ccactcgtcg gtttgaatgg caatttcgtc gggaaaataa tgatgattgg cgagcgcggg
     3841 gcggtcgtca aaaacggtgg ctttgaaatc gagttgcgct gcaagtggag cgacagcttg
     3901 ggcaatgtga cccgccccgg cgatccaaag gtggcacggt ggcgatatgg tttcgaggta
     3961 aagccaatcg ttgccatgat tcatgtcggt tgcacttgag tgtttgacgt cgaaatcctt
     4021 cctcacgccc cagacaattt ggcgctttcg ttcggcgagt tgcgaccaaa actctgcgcc
     4081 aactttgact gcatttggca ggatgacgcc aaacaccttt ccgccacaaa tcaatccatc
     4141 gtcccagccg aaatcgtgat caagaaggag atcaaacgtc gcgggttcgc cggtgcgcag
     4201 agcctggatg gcgcgttgct ggatttcggc ttcgagacaa ccgccgccga gtgtgccttg
     4261 aatgcgtcca tcggaataga agagagcttt cgcgccgacc ttttgagggc tggaaccttt
     4321 cgcgccggaa atgagcgcca acgcggcagg ttcgccgctg cgagcggcct tcgccaatgc
     4381 ctcaaagacg atgtggttca tgccggtttt tctttttgct tttgaatcgc catgaaaata
     4441 gcctcgctcg ttgctggaga agcgagggga atttcgttgg ctggcggccc gaacgcggcc
     4501 accgcatcgc ggatggcttc gcgcacggaa atggccagca tcaatggcgg ttcacccacg
     4561 gctttgctgc cgtgaatgac atttttctgc gcggcatttt gcaggaattg cgtgttgaaa
     4621 atttgcgggc ggtcgccgat ggaagggatt ttgtaagtgt cgggagaatg cgtcagcaat
     4681 cttccttggt catcccattt caattcctcg gtcgtcaacc aacccatgcc ctgcacgaaa
     4741 ccgccttcga cctgtcctcg gttcacaccc gcgttgatgg aatcgccgac gtcctgcaaa
     4801 atatcggtgc gcaacacctg catcatcccg gtgaaaccgt ccacttccac ttccgtcacc
     4861 gccgcgccat aggcgaaata atggaacggc ttgccgcgcc ctttcgcgcg gtcccaatga
     4921 atgtccggcg tccgataata gcccgtcgca ctcaggctga tgcgctggaa gtaagctttg
     4981 cgcacaattt cagcgagcgg aatcctcact tccgggtgct cgcgatcaga aacaaaacct
     5041 tccacaaaaa gcacattctc caacgacgtt tcgcgtgctg atttttcttt caaacatccg
     5101 gccgccacag gtctcaaccg ttcgcggaga atttcacacg cattcttcac cgcggcgcca
     5161 ttcaaatccg tgccggcgga tgcggcggta gccgaagtgt tcggcacttt atcagtgctg
     5221 gtcggcatga cgcggacatc ttcgtttttc acgcccaatt cctttgcggc gatggcgcgg
     5281 atgttggtgt ggatgccctg acccatctcg gtcccgccat ggttcacctg cgccgtgcca
     5341 tcctgataaa gcaacaccag cgcgcctgcc tgattgagat gcgtgacggt gaacgaaatg
     5401 ccgaacttca ccggcgtcat ggcgagtccg cgtttgcgat gcgggttttg cgcgttccac
     5461 gatttgattt cctcgcgtcg tttctcaatc ccgctcgatt ccttcaattg gttccagaga
     5521 acattgatgc ggttgtcctc gatttcctgg ccgtaatgcg tcgtgttcgt ctcgcctcga
     5581 ccgcgatata aattccgttc gcggacaatt tcgggcgtca aaccaagtcg gcgcgcgacg
     5641 cggtcaatga tctcctcgat gacgagcatt ccctgcggtc cgccgaaacc gcgaaacgcc
     5701 gtgttcgacg aaacgttgag cttcgcaact tgcccgcgaa attccacctg cgggatgtaa
     5761 taggaattat cgaggtgaaa cacggcgcgg tcagtgacgg cttgcgaaag gtcgagcgac
     5821 catccgccat tggaataaag atgcgccttc actgcgagca acattccttg cgcgtcgtat
     5881 ccgacttgaa accgggcaag gaacggatgc cgatgtccga gcagcatcat gtcctggtcg
     5941 cgattgagcc gcacacgaac ggcgcgtccg gtgtggtgcg ccgccagcgc ggccagcgca
     6001 gcgggcgttg cggcttgggt ttccttgccg ccaaaaccgc cgcccatgcg cgggctttgc
     6061 accacgacgc ggttcattgg caaatgcaag acatgcgaaa taacgttttg gacttcggat
     6121 ggatgttgag ttgaggaaca gacgaacatc gaaccgtctt cgccgcgctg ggcccacgcc
     6181 gcattggttt cgagataaaa atgttcctga ccgccgaatt cgaactcgcc ttcaaacgcg
     6241 aacggcgcgg actgcaatcc ttgcacaacg tcgccgcgcc ggatgaaatt cggttcgttg
     6301 tgaaaactgc cttcgcgcac agcttgcggg agcgacaata tcggtgtcag cggttcatat
     6361 tcaacgatca cttttgcggc ggcagcgcgg caggcttctt gcgaatcgcc gatgacgagc
     6421 gcaaccagat gcccgtgaaa aaacacttcg cggtcagcca gcaaaatttc gtcatgaatc
     6481 accgcgccga catcattgcg ccccggaatg tcctccgcca gcaacaccgc cttgatgcca
     6541 ggcatccggc gcgcttccgt ggcatcgcgt ttcaaaattt ttgcgcgcgc gtaaggcgaa
     6601 caaaccggcc acgtttcaag cattcccgca gtctggtcat ccacgtaaat cgcctcgccg
     6661 gtcacatgtt tgtgcgcgct ttcatgcggc aatggatgcg ccgaattctc gtagctgaaa
     6721 acgtgagttt tcagcgcctg ctttgcagca tcgggttttt cataaaaaaa cttctcgaac
     6781 aagtttttga ccaattcctg cctgtatcgc gcttctccac gaacatcgga aatcggagta
     6841 aattccgttc gcagggtggg cagcatttcc gcaatcaatt ccgcgctcca tttattgccg
     6901 atcaacgcct gctcggtttt acgcgcacgg acaggaaggg cagcgacacc gccgtaggca
     6961 agccgcgcat aacgcacaac accagccgcg tccagatcca ccgtgaaaca ggccgcgacg
     7021 gtgctgatgt ccatttcgcg gcgtttggaa actttgtacc attggcattt gcgtgtgaga
     7081 ttcgattgcg acgcgcctcg cggcataaga attgtcttga gcacttcgcc gggttgcaaa
     7141 gccgttttac gataggcgat aaaaaattct tccagcggca aaacgcgctc gccttgcaac
     7201 gaagccaaaa ccacttttgc atccaaggcg agcagcaccg gcgcgctgtc gccgatgggc
     7261 gaagcagtga caagatttcc gcccagggtg gcgcgattgc gaatctgtcg cgaaccaaag
     7321 acgcgcagca tgtccgccaa cgccggatat tccgcgccga gggcatcttc caattccgtc
     7381 atcgtcaccg cagcgccgat gcgccattct gagggtgatg attggagttc cttcaattcc
     7441 ggcgtggcct cgactgaaat cagcgtggga aattttttgt atttctttgt gatctccagt
     7501 ccaaggtccg ttgcgccggc gaccaaacgc gcttccggat gctcttgcat caaccgcagc
     7561 aattcatcca gtgacgcagg ccggaaaaat ttttcgccgg aaaattcgta atgtgtcgcc
     7621 gagggttcgg caaaagcgtg actcaatttc gcgacgaacg ggtcctcgcc attctgttta
     7681 tttttctgct tgaaagcttc cacggcggct tcgtgaatcg agcgataccc ggtgcagcgg
     7741 caaagattgc cgcaaagctg ggcgtcgagc tgcgaatcat tctgcaaatc cttgcgataa
     7801 tatccctcga agagcgacat gatgaaaccg ggtgtgcagt aaccgcattg cgaaccgtgg
     7861 cattcgacca tcgtgcgctg gaccggatgc aattcgccat tcccgccggc aacgccttca
     7921 accgttatca cttcgcgccc ggcaagcgat gggagcggca cgagacaact attgattgcg
     7981 cgatagcagg cgtggccatt tgaatcgcga tcaatgatgg cgaccgagca cgcgccgcaa
     8041 tcgccttccg cgcagccttc cttggagccg gtgcaaccgt ggccgcgaag atattcgagc
     8101 agggtaagat tgggagagca gtcccggacg cgaactgccc ggccattcaa aacaaactcg
     8161 atggcaccgc gcatttccat cccgcagttt agcggaacag gcggcttagg caatagcggc
     8221 ggaaggacga tgcctgcgaa gccgcacccc gcgggcgtcg attgcacatg gcactttgaa
     8281 caaagaatct ccttccggct gaagacag
//
LOCUS       DASZMM010000111         5235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1131837, whole genome shotgun sequence.
ACCESSION   DASZMM010000111 DASZMM010000000
VERSION     DASZMM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131837"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(471..1781)
                     /locus_tag="VG754_02780"
     CDS             complement(471..1781)
                     /locus_tag="VG754_02780"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWF18158.1"
                     /translation="MKTKSKSGKSSRRNPAHAATRSGAWNLAVAGLALGAPLFLCREA
                     GAVGFRLPNQDPEAIARGNAFAATADNPSAIYYNPAGITQLDGQNLSAGLYLLSAGVK
                     YEGTAGAARANGQFQPVPQLYYTRSLSDLPISFGVGVYAPYGLSMDYGNTGPFHNVAQ
                     NGSLLYATVNPVVAWKITPELSLGAGLTINYSKVDYNQGIAGLEAFGFQNTQFHFVGD
                     DYDFGFNAGALYKPCKYVSLGLNYHSETTMNFKGHSSTITSSPLVPNSSSPTTASITF
                     PQYVVGGISYRPTEKWNIEFDLDWTDWHAVKQATFNGTALGAPIPNLPFHYESTFMYE
                     LGVTRYLEEGYFLSAGYFYSENSSPDANFDPLIPDSDLHLFSVGFGHKGDRWGWAFSY
                     TLAYQPGRNVSGSSFNNPALPGSTVNGTYRILNNALNASINLKF"
     gene            complement(1741..1863)
                     /locus_tag="VG754_02785"
     CDS             complement(1741..1863)
                     /locus_tag="VG754_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18159.1"
                     /translation="MLLASALLNGFDASFLDQANLQRRNPNYEDKIEERQIIPA"
     gene            2283..3692
                     /locus_tag="VG754_02790"
     CDS             2283..3692
                     /locus_tag="VG754_02790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18160.1"
                     /translation="MSGKLDSLVTCKTSQGQSILVPVRRLTRHLVVFEINDPNCVIRF
                     SEALDSFEIKLNDQTVYSGRAVVSSVINTGTGIVCEAALKDGWINSPPASSGGNRSWS
                     AQFDEFLKQWQKNYRIAPEFKLVIADLQTMLLEMRVWLEQAEFGLQSQPAGERAEAER
                     GIINELQQPVLPALGTLCQKFEESCRDIPPEMEPVHRAYIQRQIHPLVLSAPFMNRTF
                     TKPLGYAGDYEMVNMMVRDPQEGPTLFAKLLNTFFLSTPPVVAHRNRIEYLKGILTRE
                     IARAARHGTAAKIFNLGCGPAKEIQDFIAMSALSDLANFTLLDFNEETLRYTGGKLRE
                     LIAQNHRRTGLQMVERSIVQLLKESNRPNSSLFATRYDLVYCAGLFDYINDPVCQKLM
                     DLFYNLLAPGGLLVATNVDSANPSVNWMGYVMDWHLIYRNGDQMGRIMPRQASPEECV
                     IHCEPTGVNTFIEVRKPHG"
     gene            3685..>5235
                     /locus_tag="VG754_02795"
     CDS             3685..>5235
                     /locus_tag="VG754_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18161.1"
                     /translation="MADAARIQAENPEQAFRQYEQGVRIRNYRIVCVLGIIFMPAGVV
                     LDLAVYPERWKHFLGLRLVCSALFAFIWWFVRTPLGLRTLWLMGFVVGALPSFFISLM
                     IYETAGARSPYYAGINLILLGAVLVLRWSLWNSIVVILSAIGMYLAACFLHGDVHGDD
                     SRIFFNNLYFLFVTGVIAVTGNLFYNRIRLREFILRYELDKSRGMLAESNEKLKELDQ
                     IKNRFFANISHELRTPLTLLLSPLETMMHGGKQQFDGATRNLMSIMHGNGMRLLKLIN
                     DLLDLVRLESGRMEVKREPVEMNAFVKNLMSAAQQMADSKGLMLETTVQPDLGPLMVD
                     TNKLEKTILNLVFNALKFTPKGGTVSLHVQKYGEQLVIEVEDTGVGISEKNLPHVFDR
                     FWQADNSSKRKYQGVGIGLSLVKELTEVQGGSVSVQSQEGRGTTFTVNLPFIKAEGTV
                     LPDKAEEKPQAQSAEPAPAAEAAPVPVQSEEWLTNLYRRAELFPAIGTNSDAPQATKV
                     SDNGKGQSR"
BASE COUNT         1221 a         1372 c         1503 g         1139 t
ORIGIN      
        1 tgaacgccgt cagattgcaa ccacgacggg atgcgttgct ccctctcccc aagggaccac
       61 gtaacgcgaa gcgttcgtct cctttgggga aactgacttg tggcgggctt gggaagccat
      121 tgggccggtg agggggacag cgatgtgcgc cggctcatga acacaattcg caagagccca
      181 gaaacgtgca aaatagtgag atgcgccctt tattatcgcg acagaaattt tggtgactaa
      241 aacgcaaagg gttcatgaag aggcaaccca tcgtcggcct ctttgcgtcc ttttgcggct
      301 gaattgagtt tttgttttac cggcttttgc gctttttagc ggacaaattt tttgcctcga
      361 tgctaaagga gtcaggagta gcgataaaaa attgcccgtt tcccccacgc gttgatcgtt
      421 ggagaattga gggggaacag tcgttgcgag tgggaatcga agaatcccgc tcagaacttc
      481 aagtttatcg aggcgttgag ggcgttgttg aggatgcggt aagtgccgtt cacggtgctg
      541 ccgggcaggg cggggttgtt gaaagaactt cctgaaacat tgcggcccgg ttgataagcg
      601 agcgtgtaag aaaacgccca gccccaacgg tcgcctttgt ggccaaatcc aacgctgaac
      661 agatgcagat cgctgtcggg aatgagcggg tcgaaatttg cgtcaggact ggagttctca
      721 ctgtagaaat atcccgcgct caagaaatag ccttcttcca gataacgtgt gacacccaac
      781 tcatacatga aagtggactc gtagtggaac ggaagattgg ggatgggcgc tcccagagcc
      841 gtgccgttaa aggtggcctg tttcacggcg tgccagtctg tccaatcaag gtcgaattcg
      901 atgttccatt tttcggtcgg gcgataggaa atgccgccga cgacgtattg gggaaaggtg
      961 attgaagcag tcgtggggct cgaagaattg ggaacgagcg ggctcgaagt gatcgtgctt
     1021 gaatgtccct tgaaattcat cgtggtttca gaatggtaat tcaaaccgag cgagacgtat
     1081 ttgcatggct tgtaaagcgc gccggcattg aaaccgaaat cgtaatcgtc gccgacaaaa
     1141 tgaaactggg tgttttgaaa tccgaatgcc tccaagcctg caatgccctg gttgtaatcg
     1201 accttggagt aattgatcgt caagccggcg ccgagggaaa gttcaggggt gattttccag
     1261 gccaccacgg gattgaccgt cgcgtacaac aggctgccgt tttgcgcgac attgtggaaa
     1321 ggcccggtgt tgccgtaatc catggagaga ccgtaaggcg catacacgcc aacgccgaaa
     1381 gaaatgggaa gatcactgag cgagcgggtg taatacaatt gagggacggg ttggaattgg
     1441 ccgttggcgc gggcggcgcc cgcagtgcct tcgtacttca cgccggcaga aaggagataa
     1501 agcccggcgc tcagattttg tccgtcaagc tgcgtgatcc cggcaggatt gtaataaatt
     1561 gcggaaggat tgtccgcagt ggcggcgaag gcattgccgc gagcaatcgc ttccgggtct
     1621 tggttgggca gccggaaacc gacagcgccg gcttcccggc agagaaacaa cggagcgccc
     1681 aacgccaagc cagccacggc aagattccac gcgccgcttc gggtcgccgc atgggccgga
     1741 ttacgccggg atgatttgcc gctcttcgat tttgtcttca tagttcggat tgcgccgctg
     1801 caagttggcc tgatccaaaa agcttgcgtc aaacccattc agcaaagctg atgccagcag
     1861 catgttacct ctccaaaccg accggtcatc gttgtgcgga acaaaaacca aaataatcta
     1921 ttaacattta tgaatgagca agttatagct ggttttgatg ctacaatgca agtggatttg
     1981 gccccaaacg ggactgtcgg cactcattgc agtagagtgt cttattgtcg tccaagattg
     2041 cccttaatgg gacaacagcc atcgggcact tccgaaatgt ggaagtttcc agtgcaaaaa
     2101 atgatgacaa aaactggtga accgaagctt gagcttcatc ggaaaagggt gttagaactg
     2161 attgggccga agttgtgcca aaacgaaaag cgcaagcagg accccgactt ttcacgccca
     2221 caggggttta ggcacaatta ttgcgcacga ttaacagaat gcaacagaac ggaaacacaa
     2281 aattgtcagg caaactcgac agtctggtga catgcaagac cagccagggc cagagcatcc
     2341 tagtgccagt ccggcggttg acccggcact tggtagtctt tgagataaac gacccgaact
     2401 gcgtgattcg gttctcggaa gcgcttgaca gtttcgagat caaattaaac gaccaaactg
     2461 tttattccgg tcgcgcagtg gtttccagcg tgatcaacac aggcacgggc attgtttgcg
     2521 aggcggcgct caaggatggc tggatcaatt ccccgccagc ttcgtcggga ggcaatcgct
     2581 cctggtccgc ccaattcgat gaatttttga agcagtggca aaaaaattac cggatcgccc
     2641 ctgagtttaa gctcgtgatc gcagacctgc aaacgatgct cctggaaatg cgtgtctggc
     2701 tcgaacaggc tgagtttggc ctgcaatcgc aacccgcggg cgaaagagcg gaggccgaac
     2761 gcggcatcat caacgaattg cagcagcccg ttttgccggc attggggaca ttgtgccaga
     2821 agttcgagga atcgtgccgc gacattcccc ccgaaatgga gccggtgcat cgcgcctata
     2881 tccagcgcca aattcacccg cttgtcctct ccgcgccgtt catgaaccgc actttcacca
     2941 agccgctcgg ctatgccggt gattatgaga tggtgaacat gatggtgcgc gacccgcagg
     3001 aaggccccac attgtttgcg aaactgctga acactttttt tctcagcacc ccgccggtcg
     3061 tggcgcaccg taaccgcatc gagtatctca aaggcatcct gacccgggag atagcccgcg
     3121 cggcccgcca cggcacggcg gcaaaaatct tcaacctggg ttgcggcccg gcaaaggaaa
     3181 tccaggattt tatcgcgatg tccgcgctga gcgacctcgc gaattttacc ctcctggatt
     3241 tcaacgagga aacattgcgc tacaccggcg ggaaactccg ggaactcatc gcccagaacc
     3301 accgccgcac cggactgcaa atggtcgagc ggagcatcgt gcaattgttg aaagaatcca
     3361 accgccccaa ctcgagcctg ttcgccaccc gttatgacct cgtctattgc gccgggcttt
     3421 ttgattacat caatgacccc gtgtgccaga aactgatgga ccttttttac aacctccttg
     3481 cgccgggcgg tctgctggtg gcaacgaacg tggactcggc caatccgtcg gtcaattgga
     3541 tgggttatgt gatggactgg cacctgattt atcggaacgg agatcaaatg ggccggatca
     3601 tgcccaggca agcatcgccg gaggaatgcg tcattcattg cgaaccgacg ggtgtgaata
     3661 cattcattga agtccgcaaa ccccatggct gacgcagcgc gcatacaggc cgagaacccg
     3721 gagcaagcct ttcgccagta cgaacaaggc gttcgaatcc gtaattaccg gatcgtctgc
     3781 gtgcttggga tcatcttcat gcctgcgggc gtggtgctgg acctggcggt ttatcccgag
     3841 cggtggaaac atttcctcgg gttgcgattg gtctgctcgg cattgttcgc gttcatctgg
     3901 tggtttgtcc gcacaccgct gggattgaga actctctggt tgatgggttt tgtggtgggc
     3961 gccctgccaa gttttttcat ctccctgatg atctacgaaa ccgcgggcgc gcgttcgccg
     4021 tattacgcgg gcatcaacct catcctgctc ggcgccgtgc tcgtcctgcg atggtcgttg
     4081 tggaacagca tcgtggtgat cttgtcggcg attgggatgt atctggccgc gtgcttcctt
     4141 catggcgacg tgcatggaga tgattcgcgc atctttttca acaatcttta ttttcttttt
     4201 gtcaccggcg ttattgcggt gaccggcaac ttgttttaca accgcatccg cctgcgcgaa
     4261 ttcattttgc gctacgaatt ggacaaaagc cgcgggatgc tcgcggagag caacgaaaag
     4321 ctcaaggaac tggaccagat caagaaccgt ttcttcgcga acatcagcca cgaattgcgc
     4381 acgccgctga cgttgctgct ctcgccgctg gagaccatga tgcacggcgg caagcaacaa
     4441 ttcgacggcg cgacgcgcaa cctcatgtcc atcatgcacg gcaacggaat gcgcctgctg
     4501 aaattgatca acgatttgct cgacctcgtg cgtttggaat ccgggcgcat ggaagtcaaa
     4561 cgcgagcctg tcgagatgaa tgcgttcgtg aagaatttga tgagtgcggc ccagcagatg
     4621 gctgactcaa agggtttgat gttggaaacc acggtgcagc cggacctcgg tcccttgatg
     4681 gtggacacca acaaactgga aaaaacgatc ttgaacctgg tgttcaacgc cttgaagttc
     4741 actcccaagg gtgggacggt ttccctgcat gtccagaagt acggtgagca actggtcatc
     4801 gaggtggagg acaccggcgt gggaatttcc gagaagaatt tgccgcacgt cttcgaccgg
     4861 ttctggcagg cggacaattc ctccaagcgc aagtatcagg gcgtgggcat agggctttcg
     4921 ctggtgaaag aattgacgga agtgcagggc ggcagcgtct ccgtgcaaag tcaggagggc
     4981 agaggcacca cgttcacggt gaaccttccc ttcatcaagg ccgaaggtac agtgttgccg
     5041 gacaaggcag aagaaaaacc gcaagcgcaa tccgccgaac ccgcgcctgc ggccgaggcc
     5101 gcgcccgtgc cagttcaatc cgaggagtgg ctcaccaatc tttatcgccg cgcggaatta
     5161 ttccccgcaa tcggcaccaa ctccgatgcg ccgcaggcaa ccaaggtttc agacaatggc
     5221 aagggccaat cacgc
//
LOCUS       DASZMM010000112         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1954548, whole genome shotgun sequence.
ACCESSION   DASZMM010000112 DASZMM010000000
VERSION     DASZMM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954548"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..801)
                     /locus_tag="VG754_02800"
     CDS             complement(223..801)
                     /locus_tag="VG754_02800"
                     /inference="COORDINATES: protein motif:HMM:NF042518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6567 family protein"
                     /protein_id="HWF18162.1"
                     /translation="MPLFTEDKVKALREMRRLPGSATGKANFFRGKLLAILTLAGIVA
                     LAGGCAAVAPVSSAVGAVTPSSTLQIFNTTEVRLQQKNFVVVKSNVMGQSKGFSLFGL
                     VTFVPAKFDKAMKRLNAQADLNPGTPRTLANLVMEKDSSYWILFSIPRTSVRADVVEF
                     TDNPHGQPPSGIARTDGNKQGRIRAQPRSAAR"
     gene            959..1102
                     /locus_tag="VG754_02805"
     CDS             959..1102
                     /locus_tag="VG754_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18163.1"
                     /translation="MKTKLVFGWLILVSAVALLVGCQTSNQQVQNIDLEFPPGPPLSA
                     NSP"
     gene            complement(1128..1937)
                     /locus_tag="VG754_02810"
     CDS             complement(1128..1937)
                     /locus_tag="VG754_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWF18164.1"
                     /translation="MRYIEPHAHMVSRVTDDYQAMVTAGCQAVCEPAFWAGFDRSSAD
                     GFYDYFCQLTEHEPKRAAKFGLPHFSWLCINPKESEDLKLADDVLAIIPQFLDRPNVL
                     GIGEIGLNKNSRNEVKVLEKHVNLAAEHDQLILVHTPHLEDKLKGTRLIVDLLKSDKR
                     IRTERVIIDHVEEHTVKLVLDAGMWAGMTLYPESKCSPARAVDMVEMYGNERVWMNCA
                     CDWGVSDPLAVPKTMLEMRKRGHSASAIDKLVYQNPLKFLGQCKKFKPLAD"
     gene            complement(1976..2368)
                     /locus_tag="VG754_02815"
     CDS             complement(1976..2368)
                     /locus_tag="VG754_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HWF18165.1"
                     /translation="MVDGKGRRQTEIMFPFEKLETWQKAIDFADIVYRLTKRFPDEER
                     FGLTSQMRRAAVSIASNIAEGSARYSQADFARFVEIATGSLFEVITQATIGKHQHFLN
                     ESEYQELYSAAETQGRMLSGLRRSLKSV"
     gene            complement(2368..>2606)
                     /locus_tag="VG754_02820"
     CDS             complement(2368..>2606)
                     /locus_tag="VG754_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18166.1"
                     /translation="RFKLIEIAAFMDRVERSEGAEDFRIEAFRQALTELGKGNKERAK
                     QVLLSFSDPTTEPIPAATTKAACGAYKKVDSNLS"
BASE COUNT          567 a          698 c          706 g          635 t
ORIGIN      
        1 tcttccaccg caatctcggc cacgcattga ttaaagcgcg gccaccagcg ttgtacaagt
       61 tacaatcccg tgacaggcct gttacatcac tgtgtcatcc cccacaaaat taccgagcat
      121 tcattgtgcc ggacactatt ggtcggtgac ccgcagcaat gtgggaaggt taggccgcac
      181 gaattagtct ggcgtgtccg gttcatcgcg cgttgctgcg gctcatcgag ccgcgctccg
      241 gggctgtgcg cggatgcgtc cttgcttgtt gccatccgtt cttgcgatgc cggaaggcgg
      301 ttggccgtgg gggttatcag tgaattcgac gacatcggcg cgaacggatg ttcggggaat
      361 ggaaaagagg atccaatagc tgctgtcctt ttccataacc aagttcgcca aagtccgggg
      421 agtgccggga ttcaaatcgg cttgggcatt cagccgtttc atcgctttgt cgaatttcgc
      481 gggaacaaac gttacaagcc cgaacaatga gaaacccttg ctctgcccca tcacattgga
      541 tttgaccacg acaaagtttt tttgctgcaa acgcacttca gtggtgttga aaatctgcaa
      601 tgtggatgac ggagtcacag cgcccaccgc cgagctgact ggcgcaaccg cggcacagcc
      661 gcccgccagc gcgacaattc ccgcgagggt gagaatcgcc agcagttttc cacggaaaaa
      721 attcgccttg cctgtcgcgc tgccgggcaa gcgccgcatt tctcgcaacg ctttgacctt
      781 atcttctgta aaaaggggca tatagagatt ctctggcgtg aacgttcttt tgccatgggg
      841 caaaatccca ccagcgggtc ttcttccgca tcattccggt aacattatgt gaggcgaggg
      901 gtctttagct tcgagatggc cgtgcgctgt ctgcaaacga attgaagaaa gagccccaat
      961 gaaaacgaaa ctggtcttcg gatggttgat attggtcagc gccgttgcgc tgctggtcgg
     1021 ttgccagacg agcaatcaac aagtccaaaa tattgatctc gaatttccgc ccggaccgcc
     1081 tttaagcgcg aattctccgt gattgtggcg ggacttggag accgcgttca atcggccaat
     1141 ggcttgaact tcttgcattg gcccaggaat ttgagcgggt tctggtaaac gagcttgtcg
     1201 atcgcagacg cgctgtgccc gcgcttgcgc atttcgagca ttgtttttgg cacggccaac
     1261 ggatcgctca caccccaatc gcacgcgcaa ttcatccaga cgcgttcgtt gccgtacatc
     1321 tccaccatat cgacggcgcg ggcaggggag catttcgatt ccggataaag ggtcatgccc
     1381 gcccacatcc cggcgtcgag caccagcttg accgtgtgtt cctcgacgtg gtcaatgatg
     1441 acccgctcag tgcggatgcg tttgtcgctc ttcagcaaat ccacgatgag ccgagtcccc
     1501 ttgagtttgt cttccaaatg cggcgtgtgg acaaggataa gttggtcgtg ttcagcagca
     1561 agattgacat gtttttcgag caccttgact tcgttgcggc tgtttttgtt caggccgatt
     1621 tcgccgatgc cgaggacgtt gggccggtcc agaaattgcg ggatgatggc cagcacgtcg
     1681 tcggcaagtt tcaggtcttc ggattccttg ggattgatgc acagccagga gaaatgcggc
     1741 agaccgaatt tggccgcgcg tttcggctca tgctcggtaa gctggcaaaa gtaatcgtaa
     1801 aaaccgtcgg cggaactgcg gtcaaagccg gcccaaaacg ccggttcaca aactgcctga
     1861 caaccggctg tgaccatcgc ctgataatca tccgtcaccc ggctgaccat gtgagcatgt
     1921 ggttcgatgt agcgcacgtg agatggtgga tagttgatgg ttgatggttg atggttcaga
     1981 cggatttaag ggaacgtcga agtccgctga gcatgcggcc ttgggtctct gcggcagaat
     2041 acaactcctg atattcggac tcgttcaaaa aatgttgatg tttgccaatc gtggcttgag
     2101 tgattacttc aaacagcgaa cctgtggcaa tctcaacgaa acgagcaaaa tcagcttgag
     2161 aatagcgggc tgatccttcg gcaatgttag atgcgattga aacggctgcg cgccgcatct
     2221 ggcttgtcag cccaaatcgt tcctcatctg gaaacctctt tgttaaccgg taaacgatgt
     2281 cagcaaaatc tatcgccttc tgccatgttt caagtttttc gaatgggaac ataatttcag
     2341 tttgccttct gcctttgcca tcaaccatca actcaaatta ctatcaacct tcttatacgc
     2401 cccgcacgcg gctttagttg ttgcagcggg aatcggctcc gtcgttgggt cgctgaacga
     2461 aagcaaaact tgtttcgccc gttccttgtt gcccttgcca agttccgtca atgcttgccg
     2521 aaaggcttcg atacgaaaat cctccgcgcc ttcggagcgc tcgacacggt ccatgaacgc
     2581 ggcgatttcg atgagcttga accgcg
//
LOCUS       DASZMM010000113         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2244738, whole genome shotgun sequence.
ACCESSION   DASZMM010000113 DASZMM010000000
VERSION     DASZMM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244738"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VG754_02825"
     CDS             complement(<1..345)
                     /locus_tag="VG754_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWF18167.1"
                     /translation="MNIEIINTGSELMLGFVLNTHQQWLCRQLADAGYDVARQTAVAD
                     TAPAIQQTVREALGRADFIIVTGGLGPTSDDLTRDLIAQLLGKKLHSDATILKHLENF
                     FAQRNRPMPETTK"
     gene            complement(400..687)
                     /locus_tag="VG754_02830"
     CDS             complement(400..687)
                     /locus_tag="VG754_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWF18168.1"
                     /translation="MAELNSTIHQPVRLRIMASLMVLNGNAHMDFSSLGKLLKLTDGN
                     LGAHLQTLEQAGYIRVEKAFVDRKPRTRVSLTSKGRYEFEEHVAALKQILG"
     gene            complement(691..1290)
                     /locus_tag="VG754_02835"
     CDS             complement(691..1290)
                     /locus_tag="VG754_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18169.1"
                     /translation="MNISKGEAQESLNEIQSVIEHTRKAIAYGASSSLLVLWGVIWVI
                     GYSATQFYPAYAGLAWMGLITIGGVSSWVVGMRNQSSIQSENGRRIGVFWLILFGYAA
                     VWLLLLHPAKLPQGAEWAHYQPINDRQISAFFATVPMFAYVVGGLWFGRFFVWLGAVV
                     TAMTLFGFYCLPQWFQLWMALTGGGSLIAAGLYIRKSWR"
     gene            complement(1406..>1781)
                     /locus_tag="VG754_02840"
     CDS             complement(1406..>1781)
                     /locus_tag="VG754_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18170.1"
                     /translation="LWFLVVVSVMAFTGLLFMHSLNFVLYRLTAMMPDEALREETRQF
                     STLNRVLVLGILLLGIAILLLIHYQPPLMPPLNMIILTLAYNGLWIWLLIFLSLLPLA
                     MTMALLWKIKEVILDSVFGSGR"
BASE COUNT          438 a          470 c          491 g          382 t
ORIGIN      
        1 tttggtcgtt tccggcatgg gacggttgcg ttgggcgaag aaattttcca gatgtttgag
       61 gatggttgcg tccgaatgca attttttgcc gagcaattgg gcgatcaaat cgcgtgtgag
      121 atcatcggag gtcgggccga gtccgccggt gacgatgatg aaatcggcgc ggcccaaggc
      181 ttcacgaacg gtttgctgga tggccggcgc tgtatcggcg acggcggtct ggcgggcgac
      241 atcgtaaccg gcatcggcaa gctggcggca gagccattgc tgatgcgtgt tcaggacaaa
      301 cccgagcatc aattcgctgc cggtgttgat gatctcaatg ttcacccagc gagtttaagc
      361 gcgaagacgc aaagtggaaa gtgaacttgt gcacccggct caaccgagga tttgtttcag
      421 tgcggcgacg tgttcctcga attcgtagcg gcctttggaa gtcaatgaaa cccgggtgcg
      481 cggtttgcgg tccacgaacg ctttttcgac gcggatgtag cccgcctgtt cgagggtttg
      541 caaatgcgcg ccgagattgc cgtccgtgag tttcagcagt tttccaagcg agctgaaatc
      601 catgtgcgca ttgccgttga gcaccatcag cgacgccatg atgcgcaggc ggaccggctg
      661 atgaatcgtt gaattgagtt cggccatggc ttatctccag cttttgcgga tgtacaatcc
      721 ggctgcaatc aacgaaccgc cgccggtcag cgccatccaa agttggaacc actgcggcaa
      781 acaataaaat ccaaacaaag tcatggccgt gaccaccgcg cccaaccaga cgaaaaatcg
      841 tccgaaccat aatccgccga ccacgtaggc gaacatcggc acagtcgcga agaacgcgga
      901 gatttgccgg tcattgatcg gctggtaatg cgcccactcc gctccttgcg gcaattttgc
      961 ggggtgcaac agcaagagcc aaaccgcggc atatccaaat aaaatgagcc aaaacacccc
     1021 gatgcgtcgt ccgttctcgc tctgaatgga actttgattg cgcatgccga caacccagga
     1081 tgaaacgccg ccgatggtga tgagccccat ccaggcgagc ccggcataag cggggtagaa
     1141 ttgcgtggcg gaatagccaa tcacccaaat gacgccccag agaacaagca gactgctgct
     1201 ggcgccgtaa gctatcgcct tgcgtgtgtg ttcgatgaca gattgaattt cgttcaacga
     1261 ttcctgcgct tcaccttttg aaatattcat agatttattc tttagttcca gagaactcta
     1321 tattatagag tcctctggtg tcaacaggtt tttttatttt ttttaaagag ttagccgggg
     1381 cccctcaccc aagctgcatt tcaagtcacc ggcccgagcc gaagacgctg tcgaggatca
     1441 cttccttgat tttccaaaga agggccatgg tcatcgccaa gggaagcagc gacaaaaaaa
     1501 tcaacagcca gatccacaag ccgttatacg ccagcgtgag gatgatcatg ttcagcggcg
     1561 gcatcaatgg tggctgataa tggatcagca gcaatatagc gatcccaagc aacaaaatgc
     1621 caagcacaag gacccgattg agcgtggaga attggcgcgt ttcctcgcgc agggcctcgt
     1681 ccggcatcat cgcggtgagg cgatataaca caaaattcag gctgtgcatg aagagcaatc
     1741 cggtgaaggc catcacgctc accaccacaa gaaaccataa t
//
LOCUS       DASZMM010000114         9298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2244990, whole genome shotgun sequence.
ACCESSION   DASZMM010000114 DASZMM010000000
VERSION     DASZMM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244990"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VG754_02845"
     CDS             <1..1272
                     /locus_tag="VG754_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF18171.1"
                     /translation="DNSGMIGPADANVLVSLNAGHRPTPEYVRSLRLRLNHDFPGDTF
                     YFLPADIVTQTINFGLPAPIDIQIFGRDQNGNRQIAEQLAQKIAGVPGAVDVRVQQPD
                     NEPDLQFAIDRIKAAEVGLTEQNVADGVLLSLSGSGQVQPTYWVDPLSGIEYLVNVMA
                     PQYRMDSLPAIQSMPVNASRPGTGNVQLLANVASFSRTNSPPIFSHYNVMPVIDVYSG
                     VDGRDLGGVLHDIQPLIKQAEKELPAGSTIQVSGQVDTMRSSFIGLSLGLMAAIALIY
                     LLLVVNFQSWLDPFIILTALTGALAGVIWGLHVTNTTLSVPAMMGAIMSIGVATANSV
                     LVVTFARSNLQQGMDPLNAAWHAGVERLRPVLMTALAMIIGMIPMALGLGAGGEQNAP
                     LGRAVIGGLVIATITTLLFVPVVFSLLHRRT"
     gene            1269..2507
                     /locus_tag="VG754_02850"
     CDS             1269..2507
                     /locus_tag="VG754_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF18172.1"
                     /translation="MNSPEDGRPGDKGGPPKPDGQDKAASNTQTAAKPRSHLRLIAVV
                     AAIVLVIGFFVGMIPHWRQSKAADIYTQQLAITTVAVVSPAPDKSGAGLMLPAEVRPW
                     LEASIYAQVSGYLSNWLVDIGAQVKQGQVLAEIDTPEINQQLEQARAQLILAQANLDL
                     AKITDARWQSLVKRAAVSEQEAAEKSAGRAVAAANVQATAANVQRLEDMKSFQRVIAP
                     FNGTVTLRNVDIGDLIISGSGGKQLFHLAQNGKLRVYIRVPEPYAVGISPGQSAELMI
                     SEVPGRTFPVKVVTTSKAVSPVSRTLQVELEADNSDGLIFPGSYAEVRLSDASANPRL
                     VLPSNTILFRAAGLQVGIVGTNNTVELRQVQVGRDFGRTIEILSGVTSTDRVIVNPPG
                     SLANGMVVQVVQPPPLPTAK"
     gene            2504..3994
                     /locus_tag="VG754_02855"
     CDS             2504..3994
                     /locus_tag="VG754_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWF18173.1"
                     /translation="MKPYCTNQPWQNWSPLFGLVGAMMFLCGCISVGPDYKRPEATRI
                     PAAYAEPTNGWKIAEPQAHQPKGNWWEIFGEPELNQLETEAATANQQLKAAFAALEQA
                     RAIAYVTRAGLFPNLSLAPSATRERISANEPSVLTGRPIGQGNTFNDLTMPLDLNYEI
                     DLWGRVRRSLESANAQTQASADDLGTLILTVQAEVASDYITLRSLDAELAIVRSTVEV
                     FRESLELTRERRAGGVVSDLDVAQAETVYRQARAQLPGIALQRAQFQHALAVLIGKSA
                     SSFEIPEHALTTAPPIIPPGVPSELLERRPDIAAAERRMAAANANIGVAKAAFFPTVR
                     LNGMAGFESVNASTLFAWPSRFWSVGPSLTMPVFEGGALRAGLRLTRAAYEQTVANYR
                     QIVLTAFGEVEDNLAAQNFLASEYEEQSNALRAANRQLEIANDRYRDGLVTYLEVATA
                     QNTTLGLEQTTVLLHGQQLVSTVALVKALGGGWQPMMNGHKRWNAY"
     gene            complement(4007..4273)
                     /locus_tag="VG754_02860"
     CDS             complement(4007..4273)
                     /locus_tag="VG754_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18174.1"
                     /translation="MDANSEQRRDAQLTLLSKERGARDGFSLSVGFRQFLRLHDKTLA
                     GRPFGKNTIAPVPSGMGHATQKIAGQFSPENFGTKQNEYAGMLP"
     gene            4345..7329
                     /gene="fdhF"
                     /locus_tag="VG754_02865"
     CDS             4345..7329
                     /gene="fdhF"
                     /locus_tag="VG754_02865"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HWF18175.1"
                     /translation="MNSTSPKISLTIDGTITEAAASERLVDLLNRTGPKISQVCYHPQ
                     LGPIQTCDTCMVEVDGKLVRACATIIAGGMNVVTNSPRATAAQRDAFDVILGNHMLYC
                     TVCDNNNGNCVVHNTTKLLAVEHQKIPFQTKPFKVDSTNPFYRYDPDQCILCGRCVEA
                     CQNVQVNETLSIRWEDAHPRVLWDGGKTIDESSCVSCGHCISVCPCNALMEKSMLGEA
                     GFFTKLPKPALDGMIDLVKGIEPDTGYGAILQLSEGEAAMRESLIRRTKTVCTYCGVG
                     CSFNIWTKDRHILKVEANEGPANGISTCIKGKFAWDFVNSAERLTKPLIREGGKFREA
                     SWDEALNLVARRMTEIKAESGPDSLAFISSSKCTNEESYLMQKLARAVIGTNNMDNCS
                     RYCQSPATMGLWRTVGYGGDSGSIADIENASLVLIIGSNTAESHPVLATRVKRAHKLR
                     GQKLVVADLRENEMAKRADLFFRPKPGTDLVWLSAITRYILDHGLAKTSFLDKWVNGL
                     DKYKQSLEPFTMEFASDNCGLSIDTLTKVARMIAESDGVCILWAMGVTQHSMGSDTST
                     AISNLLLVTGNYMRTGTGAYPLRGHNNVQGASDHGAMPNFLTGYQSVDDPEARARFEE
                     NWKVKLPTSKGLDNHEMIEAIYEGKLKCMYLFGEEISLVDSNANFVNESLAKLDFFVV
                     QDIFFSNTCRFADVVLPASPSLEKEGTFTNTERRIQRLYQVLEPLGGSRPDWQIIQDI
                     ANRLGANWRYQHPSEIYQEIAALTPIFSGVTYERLAGFKSLHWPVAADGTDEPLLYTK
                     RFDFPDGKARLYPLAYSQPTDPTNEEFDLHLNNGRLLEHFHEGNLTYKSEGIREKVPD
                     TFVEVSPELASERGIKSGTWVQLVSRYGQVRVRAVVTDRVEGKELYMPMNSAESPVNR
                     LTSSHADAITHTPAYKETSVRLRVLAESGENPLPRTNPRYGHPTPQRGVEVERKWKRS
                     DYRIPGSTFAQTHATKPMANGQ"
     gene            7375..7863
                     /locus_tag="VG754_02870"
     CDS             7375..7863
                     /locus_tag="VG754_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HWF18176.1"
                     /translation="MAQPIPLELPPRDPRAELQARLQNAPLEHAEALLASYDVLQGLH
                     DRGVFELLRGALGSSDKVIEIVVDASKTPDAIRGMRNILILMKIMGTMDPELLEGFAR
                     SLPEALAFTRAHESKPPGLWGLLKQFRSRNSRRALLLINSMLEAFGKNLPHEEPTNSS
                     QR"
     gene            complement(8032..>9298)
                     /locus_tag="VG754_02875"
     CDS             complement(8032..>9298)
                     /locus_tag="VG754_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWF18177.1"
                     /translation="FDPVKYIQLNEQGDAYLTLGGQLRDRYEYFKNDLFGSGPQDKDG
                     YNLFRAMLNADLHLGPNVRVFVQGISATEQDRTGGPRPSDVDEADLYQAFVDITIPFG
                     ADTSLTLRGGRQVIVFGAQRLIGVSDWTNVRRTLDGVRGTLATAGNTLDLFYAHPVRV
                     LPYSFDDDIPDTSITGIYDTWEVPGVLARAKTKLEMYAFHVTRQGITFNQTSAGESRY
                     TLGTRLTANPKPFDFDLESDYQFGRFNYQETHAFSIAAMGGYTLEAVTFAPRMFLGFD
                     IASGGSSNSPGNTFDQLFPSGHDQFGIIDAIGRQNIIDVHPGLTLKLLENKPAVQQLT
                     LLTEYRQFWRENNQDAVYTSSGSILRTSGGSNAGAIGGEIDLQVNWQINRYLNTYAGY
                     SHFFHGPFITATGPHNDIDFAYSALTFTF"
BASE COUNT         2080 a         2656 c         2563 g         1999 t
ORIGIN      
        1 gataacagcg ggatgattgg gcctgcggat gcgaacgtcc tcgtatctct gaacgcgggc
       61 catcggccca ctcctgaata tgttcgcagc ctgcgtctcc ggcttaacca tgattttccc
      121 ggtgacactt tttatttttt accggcagat attgtcaccc aaacaatcaa tttcggcctg
      181 cccgcgccga ttgacatcca gatcttcggc cgtgatcaaa acggcaatcg acagatcgcc
      241 gaacaacttg cgcaaaaaat agcgggcgtt cccggcgcgg tggatgtgcg tgttcagcag
      301 ccggacaatg aaccggattt acagtttgcc attgaccgga tcaaagcggc tgaggtgggc
      361 cttaccgaac aaaatgtggc cgatggggtc ctgctcagcc tgagcggcag cgggcaggtc
      421 cagccgacct actgggtcga tcctctatcc gggattgaat atctcgtgaa cgtcatggcg
      481 ccgcagtaca ggatggattc cttgcctgcc atccaatcca tgccggtgaa cgccagccgg
      541 cctggcaccg gcaatgtcca attgctcgcc aatgtcgctt ctttctcgcg cacgaacagc
      601 ccgccaattt tttcccatta caatgtgatg ccggtcatcg atgtttacag cggagtggac
      661 ggtcgtgacc tgggcggtgt gctgcatgac attcaaccac tcataaagca ggcggaaaag
      721 gaactgcctg ccggcagcac catacaggtg agcggacagg ttgatacgat gcgctccagt
      781 ttcatcggct taagccttgg actgatggcc gctatcgcac tcatctacct gttgctcgtg
      841 gtcaactttc aaagctggct ggatcccttc atcattctca ccgcgctcac cggggcgctg
      901 gctggcgtga tctggggatt gcacgtcacg aacaccacgt tgagcgtccc ggccatgatg
      961 ggcgccatca tgagcattgg agtcgccacc gcgaattccg tcctcgtggt cacctttgcc
     1021 cggagcaatt tgcagcaagg catggatccc ctgaatgcgg cctggcacgc cggtgttgaa
     1081 cggttgcgtc cggtgctcat gacggcgctg gcaatgatca tcggaatgat ccccatggcc
     1141 ttgggacttg gcgcaggcgg cgaacagaat gctcccctcg gtcgcgcggt catcggcggt
     1201 ttggtgattg ccaccatcac cacgctgctt ttcgtgccgg tggtattcag cctcttacac
     1261 cgtcgaacat gaattcaccc gaagatggca gaccaggaga caaaggaggg ccgcccaaac
     1321 cggacgggca ggacaaggcc gcttccaata cgcaaacggc tgcgaagcct cgttcccatc
     1381 tcaggcttat tgcggttgtg gcggcaatag tgctcgttat tggatttttc gtcggcatga
     1441 ttccccactg gcgccaaagc aaagcggccg acatctatac ccaacagttg gcaatcacca
     1501 ccgtagcggt tgtttcgccc gcccccgaca aatccggcgc gggactcatg ctgccagccg
     1561 aggtcagacc ctggcttgag gcttccatct atgcccaagt cagcggttat ctcagcaact
     1621 ggctggtgga cataggcgca caggtcaaac aaggccaagt gcttgccgaa atcgacacac
     1681 ccgagataaa tcagcaactc gaacaggcgc gcgcgcagct tattttggca caggcgaatc
     1741 tcgatctcgc caagataaca gatgcccgat ggcaatcctt ggtgaagcgg gccgcggtct
     1801 cggaacaaga ggcggccgag aaatcggccg gccgtgcagt cgccgcggcc aatgtccagg
     1861 ccaccgccgc caatgttcaa cggctggaag acatgaaatc ctttcagcgc gtgattgcgc
     1921 ccttcaacgg gacggtaacg ttgcgcaacg tggacatcgg ggatttgatc atctcgggca
     1981 gcggcggcaa gcaactgttt catttggccc aaaacggcaa gctccgcgtt tacatccgcg
     2041 tgccggaacc ctatgccgtc ggcatcagtc ccggccaatc agccgagttg atgatttctg
     2101 aagtgcccgg tcggactttc ccggttaaag ttgtcaccac ttcgaaagca gtgtccccgg
     2161 tttcacgcac gctgcaagtt gaactcgagg ccgataattc tgacggtctg atttttcccg
     2221 gcagttacgc agaggttcgt ttgagcgacg cgagcgctaa tcccagactg gtgctgcctt
     2281 cgaataccat attgtttcgc gccgcgggcc tgcaggttgg aatcgtgggg accaacaata
     2341 ccgtcgaatt gcggcaagtg caggttggac gtgatttcgg gcggacgatt gaaattcttt
     2401 caggcgtaac ttcaacagac cgtgtcatcg tcaatcctcc gggatcgctg gcaaatggga
     2461 tggtcgtgca ggtggtgcaa ccaccacccc ttccgactgc gaaatgaaac cttattgcac
     2521 aaaccagccg tggcaaaatt ggagcccgct ctttggcctg gtcggcgcga tgatgtttct
     2581 ctgcggctgc atctctgttg ggccggacta caaacgtccg gaagcaacgc gcatccctgc
     2641 cgcttatgca gagccaacca atggctggaa aatcgctgaa ccgcaagctc accaacccaa
     2701 agggaactgg tgggagatat ttggagagcc ggagctgaac caattggaaa cagaggccgc
     2761 tacggcgaat caacaattaa aagctgcgtt tgccgcgctc gagcaggccc gtgcgattgc
     2821 ttacgtgaca agagcgggac tgtttcccaa tctttcactt gcaccgtcag ccacgcgcga
     2881 gcgtatttca gccaatgagc cgtcggtgct cacaggccgg cccatcggcc aaggaaacac
     2941 cttcaatgat ttaaccatgc cgctcgatct gaattacgaa attgatttgt ggggacgagt
     3001 gcggcgcagc ctggagtcgg ccaatgctca aacacaggcc agtgcggacg atctcggaac
     3061 cctcatattg actgtccagg ctgaagtggc gtcagactac atcacgttgc gctcgcttga
     3121 cgctgaactg gccatagtgc gctccaccgt cgaggtgttt cgtgaatcgc ttgaattgac
     3181 tcgtgagcgc cgggccggag gggtcgtcag cgatctggat gttgcgcagg cggaaacggt
     3241 gtatcgtcaa gcccgcgccc aacttcccgg gatcgcgttg cagcgcgcgc aattccaaca
     3301 tgccctggct gtgttgatcg gcaaatccgc ttccagcttt gaaattccgg agcatgcgct
     3361 cacaaccgcg ccgccgatca ttccaccggg cgtgccctct gaactgctcg aacgtcgtcc
     3421 cgatattgcc gccgcggagc gacgcatggc agccgccaat gccaatatcg gcgtggcaaa
     3481 ggcggccttc ttccctaccg ttcgtctgaa cggaatggct ggctttgaaa gtgtgaatgc
     3541 aagcactctg tttgcctggc ccagtcggtt ttggtctgtt ggcccttccc tgacaatgcc
     3601 ggtcttcgaa ggaggagcac tgcgcgcggg gttgcggttg accagggcag cttacgaaca
     3661 gaccgtcgcc aattaccgcc agatcgttct caccgcgttt ggcgaagtgg aagataatct
     3721 ggctgcgcag aattttctcg ccagcgaata cgaagagcaa tccaacgcgt tgcgcgccgc
     3781 caatcgccaa ctcgagatcg ccaacgatcg ttatcgtgac gggcttgtca cctatctgga
     3841 agtcgccact gcgcagaaca ccaccctcgg ccttgagcag acaacggtgc tgttgcacgg
     3901 ccagcaactt gtatccacag tcgcgctggt caaggctttg ggcggaggat ggcaaccaat
     3961 gatgaacggc cataagagat ggaatgctta ctgagttcgc aaaaattcat ggaagcattc
     4021 cagcgtattc attctgtttc gttccaaaat tttctggtga aaattgtccg gctatttttt
     4081 gtgttgcatg gcccatgcca gatggtacag gtgcaatcgt gtttttcccg aatggccgcc
     4141 ctgccagggt tttatcgtgc aatcgcagga attgtcgaaa gccaactgac aagctgaatc
     4201 cgtcgcgcgc gcctcgctct ttcgacagca aagtcagttg ggcgtcccgc cgctgctcgg
     4261 aattggcatc catctgtgct aggttgttgg gctgtacggg ttggcactgt ttttgcaaag
     4321 gatgatcccg tggataaagg attcatgaac tcgacctcgc caaaaattag tttaacaatt
     4381 gacggcacga tcaccgaagc agccgcatcg gaacgcttgg tggatctgct gaaccggaca
     4441 ggccccaaaa tctcgcaagt ctgttatcat ccccaactcg gtccgatcca gacttgcgac
     4501 acctgcatgg tcgaggtgga cggaaagctg gtgagggcgt gcgcgacaat cattgcgggc
     4561 ggtatgaatg tcgtgaccaa ttcaccccgc gcaaccgccg cccagcgcga cgccttcgac
     4621 gtgattctgg gcaaccacat gctttattgc acggtgtgcg acaataacaa tggcaactgc
     4681 gtggtgcata acaccaccaa gctgctcgca gtcgagcacc agaaaattcc attccagacg
     4741 aaacctttca aggtggactc gacgaatcca ttttatcggt atgaccccga ccaatgcatt
     4801 ctttgcggcc gctgtgtcga ggcatgccag aatgtccagg taaacgagac gctttcaatc
     4861 cgttgggaag atgcgcaccc gcgcgtgttg tgggacggcg gcaagacgat tgatgagtcg
     4921 agttgcgttt cctgcggtca ttgcatcagt gtttgtccct gcaatgcgct gatggaaaaa
     4981 tccatgctcg gcgaggcagg ttttttcacc aagctgccca aacccgcgct ggacgggatg
     5041 attgacctgg ttaaaggcat cgagccggac accggttacg gcgcaatcct ccagctttcc
     5101 gagggcgagg ctgccatgcg ggaatcactc attcgccgca ccaagacggt ttgcacttat
     5161 tgcggtgtcg gttgcagctt caacatctgg accaaggacc ggcatatttt gaaggtcgag
     5221 gccaatgaag gtccggccaa tggcatttcg acttgtataa aaggaaagtt cgcatgggat
     5281 ttcgtgaaca gcgctgaacg gctcaccaag ccgctcatcc gagaaggcgg caagtttcgc
     5341 gaggccagtt gggacgaagc gttgaacctg gtcgcccgcc ggatgactga gatcaaggcc
     5401 gagagtggcc ccgattcgct tgcgttcatt tcttcatcca aatgcacgaa tgaagagagc
     5461 tatctcatgc agaagctcgc gcgcgcggtg atcggcacca acaacatgga caactgttcg
     5521 cgttactgcc aatcgcctgc gacaatgggt ctctggcgga cggtgggtta cggcggtgat
     5581 tccggttcca ttgcggacat tgagaacgcg agcctcgtgc tcatcatcgg gagcaacacc
     5641 gcggagagcc atcccgtcct ggcgacccgt gtcaagcggg cccataaatt gcgcgggcag
     5701 aaactcgtcg tggccgattt gcgcgagaac gaaatggcca aacgcgccga tctctttttc
     5761 cgcccaaaac ccgggaccga ccttgtctgg ctttcggcca ttacgcgtta cattctggac
     5821 catgggctcg ccaagacttc attcctggac aagtgggtga atggtttgga caaatacaag
     5881 caaagtcttg aaccgttcac gatggagttc gcctcggata attgcggctt gagcatcgat
     5941 accttgacga aagtggcgcg catgattgcc gagtcggacg gagtgtgcat tctctgggcg
     6001 atgggcgtca cgcaacacag catgggctcg gatacttcca ctgccatctc aaacctgctg
     6061 ctcgtcaccg gcaactatat gcggaccggc acgggcgctt atccgctgcg cggccacaac
     6121 aacgtccaag gcgccagcga ccacggcgcc atgcccaact tcctgacggg ctatcaatcg
     6181 gtggacgatc cggaggcgcg ggcgcgcttt gaagaaaatt ggaaagtcaa actcccgact
     6241 tcgaaagggc tcgataacca cgaaatgatc gaggccattt acgaaggcaa actcaaatgc
     6301 atgtatttgt tcggcgagga aatcagtctt gtggattcaa atgccaactt cgtcaatgaa
     6361 agcctcgcca agctcgattt cttcgtcgtg caagacatct tcttcagcaa cacctgccgg
     6421 tttgcggatg tggtattgcc cgccagcccc agcttggaaa aggaaggcac ctttaccaac
     6481 accgagcggc gcatccaaag actttatcaa gttctcgaac cgctcggcgg gagccggccc
     6541 gactggcaaa tcatccagga catcgccaat cgtctcggcg ccaactggcg ttaccagcat
     6601 ccgtccgaaa tctatcagga gattgccgcg ctgacgccaa tcttctccgg cgtgacctac
     6661 gaacggctcg caggcttcaa gtctttgcac tggccggtcg ccgcagatgg aacggatgaa
     6721 ccgctgcttt acacgaaacg tttcgatttc cccgatggca aggcccgact ttatcccctg
     6781 gcttattctc agccgaccga tcccacgaac gaagagtttg acctgcatct caacaatggc
     6841 cggctgcttg aacatttcca tgaaggcaat ctcacctaca agagcgaggg catccgcgaa
     6901 aaagtgccgg acactttcgt tgaagtctcg ccggaactcg cctcagagcg cgggataaaa
     6961 agcggaacct gggtgcaact tgtctcgcgt tatggtcagg tgcgggtgcg cgccgtggtg
     7021 actgaccggg ttgagggcaa ggagctgtac atgcccatga actccgccga aagcccggta
     7081 aaccgcctca ccagcagcca cgcggatgcg atcacacaca caccggctta caaggaaaca
     7141 tccgtccgcc ttcgtgtgct cgcggaatcc ggcgagaacc cattgcccag gaccaatccc
     7201 cgttacggtc atcccacgcc acaacgcgga gttgaagtgg aacgaaaatg gaagcggtcg
     7261 gattatcgga ttcccggaag cacgttcgcc cagactcacg caaccaaacc aatggcaaac
     7321 ggacagtgaa aactgtctct gaccatcaat caaccatcaa ccctcaaaaa ttccatggcc
     7381 caacccattc ctctcgaatt gcccccgcgc gatccgcgtg cggaactgca agcgcgcctg
     7441 cagaacgctc ccttggagca cgccgaagct ttgctggcct cctacgatgt cctacagggg
     7501 ctccatgatc gcggcgtctt cgaactgtta cgcggcgccc ttggttccag tgataaggtc
     7561 atcgaaattg ttgttgacgc ctcgaagact ccggacgcga tccgcgggat gcggaacatt
     7621 ttgattctga tgaaaatcat gggaaccatg gaccccgaat tgctggaagg tttcgcccgt
     7681 tcgttgcccg aagccctggc tttcaccagg gcgcatgaat cgaaaccacc cggcctctgg
     7741 gggcttttga agcaattcag aagcagaaat tctcggcggg ccctgctcct gatcaattcc
     7801 atgctcgaag cctttggcaa aaacctgccg cacgaggaac cgacaaattc atctcaacga
     7861 taatcgggct gataagactc tgatatctca ggtgcctgcg cggagatttc gtcgctcaag
     7921 aacatcgccc gcaatctgcc gcgtcctttc aagaaatcaa acgcgccggg cccaaatgcc
     7981 tgctccatcc aatggcatga atggagttct gcttggattg aagtggatcg cctagaaggt
     8041 gaaggtcagc gccgagtagg cgaaatcaat gtcgttgtgc ggcccggtgg ctgtaatgaa
     8101 aggcccgtga aaaaagtggc tgtaaccggc gtatgtgttg agataacgat tgatctgcca
     8161 gttcacctgc aaatcaattt caccgccgat tgcgccggcg ttgctcccgc cgctggttct
     8221 caagatgctc cccgaagaag tataaacagc gtcctgatta ttctcccgcc aaaattggcg
     8281 atactcggtg agcaacgtca attgttggac agcgggtttg ttttccagca acttcaaggt
     8341 caagccggga tgcacgtcaa tgatgttttg ccggcctatg gcgtcgatga tgccgaattg
     8401 atcgtgcccg gaaggaaaaa gctggtcgaa tgtattgccc ggactattgg agcttccacc
     8461 gctcgcaata tcgaatccaa gaaacatgcg cggagcgaag gtcaccgctt cgagcgtata
     8521 tccgcccatc gcagcaattg aaaatgcatg ggtttcctgg tagttgaacc ggccaaattg
     8581 ataatcgctt tcgagatcga agtcgaacgg ttttggattg gcggtcagcc gtgtgcccaa
     8641 ggtgtaacgg ctttctcctg cgctggtctg gttgaaagtt atgccctgcc gggtgacatg
     8701 gaaggcatac atctccaact tggtctttgc ccgtgccagc acgccgggaa cctcccaggt
     8761 atcatagata cccgtgatgg atgtgtcggg aatgtcatcg tcaaaactgt atggcagcac
     8821 ccgcactgga tgcgcataaa acaaatccag tgtgttcccc gccgtggcca gcgttcctcg
     8881 cacaccgtct aacgtgcggc ggacgttcgt ccaatcggac actccgatga gtcgttgcgc
     8941 gccaaagaca atgacctgcc tgccaccgcg aagtgttaaa ctggtgtcag caccaaatgg
     9001 aatggtgatg tcaacaaaag cctggtacag atcggcttcg tcaacgtcgc ttggccgcgg
     9061 cccgccggtg cggtcttgct cagtggcgct gattccttgg acaaacaccc gcacattcgg
     9121 gcccaaatgc aggtctgcat tcagcattgc gcggaacaga ttgtatccgt ccttgtcctg
     9181 gggaccgctg ccaaacaaat catttttgaa gtattcgtag cgatcgcgca attgtccgcc
     9241 cagcgtgaga tacgcgtcgc cctgctcgtt taattgaata tatttaactg gatcgaag
//
LOCUS       DASZMM010000115         5561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4432751, whole genome shotgun sequence.
ACCESSION   DASZMM010000115 DASZMM010000000
VERSION     DASZMM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4432751"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VG754_02880"
     CDS             complement(<1..315)
                     /locus_tag="VG754_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18178.1"
                     /translation="MKNGTLKVILPFATFALICGILYVFFLKGLDVPAPMVNPKPALT
                     RPVPKPELPDPVFGSFGPADSYMPGPGWAAGTCAHGESFVPARSGSLNAIKIAIEPPE
                     GGL"
     gene            complement(344..940)
                     /locus_tag="VG754_02885"
     CDS             complement(344..940)
                     /locus_tag="VG754_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18179.1"
                     /translation="MKTKSDFLEMMKRLKVKRAVAVGTGVMMTIAASMIMFAQTNKEA
                     ADPFGGHDTADGKTLIVPLESVGPLRREMTSNEVVAILGQPERMQGKIMVYGKTFGLA
                     IACKTPVGVAAVFCGDSSGDGPGVKAFKCRTKEGIGMESTRAEIIKAFGQPTSAKSGD
                     AGLHQEELVYQPLGLTFYLQNGKVYNILVDFRKTHQPQ"
     gene            complement(951..2327)
                     /locus_tag="VG754_02890"
     CDS             complement(951..2327)
                     /locus_tag="VG754_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF18180.1"
                     /translation="MNDEAKQLRRFAEQGDEDAFNEIVASHFALVYSTALRQLNGDAQ
                     LAQDVAQIVFMDLARKARWLPLDIVLAGWLYKAARLSAAKVVRTEQRRRSREQEASQM
                     KYHETETPPDWERLRPVLDAAIGKLNAKDRDAVLLHYFERKSFRAVGSFLGLSDDAAQ
                     KRVSRALDKLRAILIRGGVGVSISSLSTFLSAGTVPLAPAGLALTVAKSSLRQAAAMG
                     PSSWTEILIQNLASAKAKLTAAGLLIFLCGGVTYLVYGLHPVQAGAFTPVDLTAYYNG
                     GLDKSWTPEYGSNYLAALGEGRHDLKRVPFEIRGVVQLEGGEWKRRGYSYPETVEGIH
                     VGMSGRRIHLLHANSAFDDPKGTTVASLVLHYSDGDQTRFDIRQGVEVLDWWEWPHAP
                     VKRPTDANTIVAWTGSNPAAEHQGARIRLFETAFLNPHPEKEIQSIDYDSAMAASAPF
                     MVALTIER"
     gene            complement(2685..3050)
                     /locus_tag="VG754_02895"
     CDS             complement(2685..3050)
                     /locus_tag="VG754_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18181.1"
                     /translation="MSTKTPECWASDPNAEALRIEVSPERSLLLPYDQFMFAELTSGG
                     DQQHLKLVFAAHEVSIRGHHLRRIETVMQRMESSLLVKLPSSQRSLITEGQPVILEII
                     VTEVESGQKRAETTSGASP"
     gene            3043..3243
                     /locus_tag="VG754_02900"
     CDS             3043..3243
                     /locus_tag="VG754_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18182.1"
                     /translation="MLIFVCVLMVMTASQFQTTHVVCGSTTETEKITVQPIPKPKASA
                     RNKARRKFHRQWPMTGADDILA"
     gene            3985..4812
                     /locus_tag="VG754_02905"
     CDS             3985..4812
                     /locus_tag="VG754_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18183.1"
                     /translation="MGFTLIELLVVIAIIAILASLLLPALAKAKLRAKAAACISNQKQ
                     LILAWTMYAGDSQEKVVNSENSVNAKGDIPWRYSPPVPMPNTAGLSGQDVDRLLLQTG
                     YQQGALFQYAANFNVLHCPADARANNPYVPNPSRPPGSYAWGSYSILSTLNGLEGQIF
                     KLTDISHLSDRYVWIEENDPRGENLGSWEINYGTPPKFSNAGFIDSTASWHGRTSTFS
                     FADGHAENHGWLDPETIAYALSMDPNKYFNSSLVPTFAQCPHDLFFLANGCATQQNP"
     gene            5041..>5561
                     /locus_tag="VG754_02910"
     CDS             5041..>5561
                     /locus_tag="VG754_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18184.1"
                     /translation="MALLAATALLPALPHNGLCAENSDPVLDLLREKGLITEGDAARV
                     KAELDARRTNELAQLPASKWMIGKGVKNIELFGDIRLRFEDRTVTDPAGGRIALNRLR
                     YALRLGLRGDAFDDFYYGFRLDTAANPRSPWVTFGTSASGTPYQGPFGKSQAGLNVGQ
                     IFLGWHPEDWVNIT"
BASE COUNT         1290 a         1569 c         1420 g         1282 t
ORIGIN      
        1 gaggcctccc tccggaggct cgattgcaat cttgatggcg ttcagcgatc cagagcgtgc
       61 aggcacaaac gactcgccgt gcgcacaggt tccagccgcc caacccggcc ccggcatgta
      121 gctgtctgct ggtccgaagg agccgaaaac cgggtctggc aattccggtt ttggaaccgg
      181 tctggtaagc gctggttttg gatttaccat cggcgccgga acatccagtc ccttgagaaa
      241 aaagacgtaa agaatgccac agatcagcgc aaacgtcgca aaagggagga tgactttgag
      301 cgttccattc ttcataagtg ggacatcaca ttaattccgg ctttcactgt ggttggtggg
      361 tttttcggaa atcaaccagg atgttgtaca cctttccgtt ctgtaaatag aacgtcagac
      421 cgagtggctg atagaccaat tcctcctggt gaagtccggc atctcctgat ttggccgagg
      481 tgggttggcc gaaagccttg atgatttctg cacgagtgga ttccatcccg atgccctctt
      541 tggtgcggca tttgaaagct tttacgccgg ggccgtcgcc agaggaatcg ccgcaaaaaa
      601 cggcagcgac tcctaccgga gttttgcaag caatcgccaa accaaatgtt ttgccataga
      661 ccattatctt gccttgcatt ctctccggct ggcccagaat cgccaccact tcgttgctgg
      721 tcatctcgcg gcgcaacggg ccaacagatt ccaatggaac aatgagcgtc tttccgtctg
      781 ccgtatcatg gccgccgaaa gggtcagccg cttccttatt ggtttgtgcg aacataatca
      841 ttgaggcggc gatagtcatc atcacccccg tgccaacggc taccgcgcgt ttcaccttca
      901 gccgtttcat catttccaaa aaatcactct tagtcttcat aaaattattc tcatcgctca
      961 atggtaagcg ccaccatgaa cggcgcggac gcggccatgg ccgagtcata atcaatggac
     1021 tggatttcct tctccggatg cgggtttagg aaagccgtct caaaaagccg aatccgcgcg
     1081 ccctgatgtt cggcggccgg attgctgccg gtccacgcaa caatagtatt tgcatctgtc
     1141 ggtcgtttaa ctggcgcgtg cggccattcc caccaatcaa gcacctcaac tccctggcga
     1201 atatcgaatc gggtttggtc accatcggaa taatgcaaga ccaagctggc tacggttgtt
     1261 cctttcgggt cgtcgaaggc cgaattggca tgtaaaagat gaatcctgcg gcccgacatc
     1321 ccaacatgga ttccttcgac ggtctctgga taactgtagc cccgtcgttt ccattcgcct
     1381 ccttcaagct gaacgacacc gcgtatctca aatggcactc gtttcaagtc gtgccgcccc
     1441 tcccccaatg cagccagata attactccca tattccggtg tccagctttt atccaaaccc
     1501 ccgttgtaat aggcggtcaa atccacagga gtgaacgctc cagcctggac cggatggagc
     1561 ccataaacca gatacgtcac gccgccacac aagaagatta gcagacctgc ggcggttagc
     1621 ttggctttgg ctgaggcgag attttgaatt aatatctccg tccaacttga cgggcccatc
     1681 gctgccgctt gtctcaggga ggatttagcc actgtcaaag ccaagccggc aggcgctagg
     1741 ggcacggttc cagcactaag gaaagttgac aaagatgaaa tcgatacccc cactccgcca
     1801 cggatcagga tcgcgcgcag tttgtccaaa gcccggctaa cccgcttttg cgccgcgtcg
     1861 tcgctcaggc cgaggaagct gccaacggcg cgaaaactct tccgctcaaa atagtgcaga
     1921 agaaccgcgt cgcgatcttt ggcgtttaac ttgccgatgg ccgcgtcgag caccgggcga
     1981 agccgctccc aatcgggtgg cgtctcggtt tcatggtatt tcatctgcga agcctcctgt
     2041 tcacgactgc gtcgtcgttg ctcggtccgg accactttgg cggccgacaa ccgcgccgcc
     2101 ttgtaaagcc aacccgccag aacgatgtcc aaaggcagcc agcgcgcctt gcgggcaagg
     2161 tccatgaaca caatctgcgc gacatcctga gcgagttgcg catcaccatt gagctgacgc
     2221 agggcagtgg aatagaccaa ggcaaaatgg ctcgcgacga tttcgttaaa agcatcttcg
     2281 tcgccctgtt cagcaaatct acgcagttgt tttgcttcgt cgttcatctt tgcccgcact
     2341 atagcacccg cagaaagaga attccgacag caaatccgtc cgcaaccgaa acctcacatt
     2401 gtccggctga cggaccggtt catctcttgc gccagccatt ctgcttccgg cttgctggcg
     2461 acgcaccgcc gctggtcatc gcctttccgg cggcccaatc gtcgcgagcc agccagggct
     2521 tttcgagttt cactgccatg ctgattccct catagcaccc gccacatgag aaatccgaca
     2581 agaaatcgtt gttcgttaga aaacccttgg cccgaagctg catttgatac tcaatttgga
     2641 tgaccgatgc gttaggtcgc tcataccctg ccactcgcct cgtattacgg tgatgctccg
     2701 ctcgtcgttt cggcacgctt ctgccctgac tccacttcgg ttacaataat ttcaagaatc
     2761 accggttgac cttccgttat cagagatcgc tggctgcttg gcaacttgac gaggagcgac
     2821 gattccatcc gctgcatgac cgtctcaatt cggcgtaaat gatgcccacg aatcgaaacc
     2881 tcgtgagcgg cgaagaccag cttgagatgt tgctggtctc cgccactggt caattcggcg
     2941 aacatgaatt ggtcgtacgg taacagcagc gaacgctctg gcgaaacttc aatgcgtaat
     3001 gcctcggcgt tcgggtcgct ggcccaacat tcgggtgttt ttgtgctcat atttgtgtgc
     3061 gtgttgatgg tgatgacggc gagccagttt cagacgacac atgttgtctg cggctcgacg
     3121 accgaaacgg agaagataac cgtgcagccg atcccaaagc cgaaggcgtc cgcgcggaac
     3181 aaggctcgtc gcaaattcca tcgccagtgg ccgatgaccg gagcggatga cattctcgcg
     3241 tagttgctgc ttgtctggtg ggcggacgcg ctagattctg gaaaaaatga gtatggataa
     3301 cgaaaccctc gcaagtcttt gagcatcaat aagagatttt tttgatgcct gtgctagttt
     3361 ttgtgctaga aaccagtctt tttcaatttg gcaatttcta tgcaggattg gtgaccaaaa
     3421 ccacgattta aatcaacaat acaaaggcaa aaatgaaaca acttgaaacg tgttgaagac
     3481 ctctgaaaat gatgctttta aaaacctgaa tctagcgcgt ctgccaattc cgccacgtgg
     3541 gcattcgtga acgcagcaac acaccatagc agatgcatcc cctggaaggc aagcttgcaa
     3601 tggcgcgcga aaacttcaat gaaaaccgat gaaatcaagc gcggggctga gcagccttgc
     3661 cattgaattt ttcagcctgc gtccttgtca cctgacgcaa cgggtcttca aaacagccga
     3721 tgttcaatat tcttcgctga acaatcttgc acgacttccg tgtcgaatgg tgtttgtttg
     3781 tcatattgac accaactgcc cttggccatg caaaatatga cacagctcaa caggactgaa
     3841 gcaaaccggg gtcgcggtgg catatccaat cggagccggc tttgcggttt caattgggca
     3901 atgtccgagc acaagccagg acgcgagcaa gcaaaaatga aagtttattc cacgaagtca
     3961 ggttgcactg cccatgaatc acgaatgggg tttacgttga ttgagttgct ggtcgtgatc
     4021 gccatcatcg caatcctggc ttccctgctt ttgcccgcgt tggcaaaagc caaactccgg
     4081 gcaaaggccg cagcgtgcat aagcaatcaa aagcagctca ttttggcgtg gacaatgtat
     4141 gcaggggaca gtcaagagaa agtggtgaat tcagagaatt cagtgaatgc gaaaggcgac
     4201 attccctggc gttattcgcc gcctgtccca atgccgaaca ccgccggcct gtccggacaa
     4261 gacgtggatc gtctgctgct tcaaacaggc tatcaacaag gcgcgctttt ccaatacgct
     4321 gccaatttca acgtgttgca ttgtcctgcc gacgccaggg caaacaatcc ctacgtgcca
     4381 aatccgtccc gcccgccggg aagttacgct tggggaagtt attccatcct gagcaccctt
     4441 aatggcttgg agggacagat attcaaactg acggacattt ctcatctcag tgaccgttac
     4501 gtgtggatcg aagaaaacga cccgcgcggc gaaaacttgg gttcgtggga aattaattac
     4561 ggaacccctc caaagttttc aaacgcgggt ttcatcgatt ccaccgcctc ctggcatggt
     4621 cgcaccagca cctttagctt cgctgatggt cacgcggaaa atcatggttg gctggatcca
     4681 gaaaccattg cttacgcgtt gagcatggat ccaaacaagt atttcaacag ctcgttggtc
     4741 ccaacttttg cccaatgccc ccatgatctt ttctttttgg cgaatgggtg tgccacgcaa
     4801 caaaaccctt gattctgcgc gtcaagagtt cctggccatc atggcttgaa caccaatcgg
     4861 acgcctcgaa aacgcaagac atgtcctcca acaggatatt tgttgccgaa atgtaacaat
     4921 tccaccacat ggctgtcact ttgccttttt accttccgcg caatgttaac acctaatcag
     4981 aaaaccaaat cgataaactg tatctctcgc tcgactcgca gcccgcaccg gagcaaatgg
     5041 atggcgctgc tcgccgcgac ggcgctgctg cccgcattgc cccataatgg gctttgcgct
     5101 gagaattccg accccgtgct cgatttgctt cgggaaaaag gtctcatcac cgaaggcgat
     5161 gccgcccggg tcaaagcgga actcgatgcc cggcgcacga atgaactggc gcaactcccg
     5221 gcttccaaat ggatgatcgg caagggcgtc aagaacatcg aattgttcgg cgacatccgt
     5281 ctccgttttg aagaccgcac ggtgaccgat cccgccggtg gaaggattgc gctgaaccgg
     5341 cttcgttatg cgttgcggtt gggattgcgc ggtgatgcct tcgatgattt ttactacggt
     5401 ttcaggctgg acaccgccgc caatccgcgt tcgccgtggg tgacgttcgg cacatcggcc
     5461 agcggcacgc cgtatcaagg gccgttcggc aaatcgcagg cgggcctgaa cgtcgggcaa
     5521 atttttctcg gctggcatcc ggaagattgg gtgaacatca c
//
LOCUS       DASZMM010000116         8449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_34519, whole genome shotgun sequence.
ACCESSION   DASZMM010000116 DASZMM010000000
VERSION     DASZMM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34519"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1350
                     /locus_tag="VG754_02915"
     CDS             172..1350
                     /locus_tag="VG754_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18185.1"
                     /translation="MSSTSPAPTIAYLAQGKVRLKIGTAAPRTLESAYGNSIREKEIR
                     AQQKHSWKSGGGGSPFGGPVLWGKSSMQQDVPLAITSICGGKEKGGLLYSMESGSLCA
                     LLEAGELGADERRLWNDNRTRIRHLSISRQTGDLAFGVLHENGTANIGLRLHGESGVK
                     ELTEGDSFDTAPHWVPGEGRKLVFQSAGIGRNREGHFLALGAFSIQQLDIEAGDMTTL
                     LEEQKYDHLAPQFLEDGTLLYIRRPYTLNEGMHPLRTMKDFFLFPFRLLFAVFQFLNF
                     FSKLFTGRQLTTDGGAKAREMNMRQMMIWGNLVNAQRPARPEEEGVDLIPKSWELHQR
                     SPDGKTKVLATGVLAYDVAPDGLIVYTNGNALFLLHPDGRKERLFNESMIEQVFFVPE
                     "
     gene            1712..3220
                     /locus_tag="VG754_02920"
     CDS             1712..3220
                     /locus_tag="VG754_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWF18186.1"
                     /translation="MNLSDYELGGFFDEMFGENGQPRAATRPLARNMQSLADDELVNR
                     QLAADRALVQAGITFNVYGESAGVEKTLPFDLIPRIVTATEWERIERGLKQRIQALNL
                     FINDLYHDRKIIKDKAVPEEIISSSKGFREQCIGMNPPRGIWCHITGTDLVRHRDGQV
                     YVLEDNLRCPSGVSYVLENRRLMKSLFPEVFAESHIRPVSNYPIRLRDMLEHLSPNGV
                     PEPRVVLLTPGVYNSAYFEHSFLAQQMGVELVEGGDLVVSEGYVWMRTTHGFERVDVI
                     YRRVDDDFLDPESFRADSMLGVPGLMQVYKSGNVALANAPGTGVADDKVVYAYVPRIV
                     KYYLGEEIILPNVPTYICAEQKDLKYVLEHLDELVVKAANESGGYGMLIGPHASAAER
                     AEFAKKIQEHPRNYIAQPTLALSRVPTLVDGQLAGRHVDLRPYILYGKEIFVMPGGLT
                     RVALKKGSLVVNSSQGGGSKDTWVLADNPSVVEQDQKQDQKQSATSVAGAVV"
     gene            3220..4209
                     /locus_tag="VG754_02925"
     CDS             3220..4209
                     /locus_tag="VG754_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWF18187.1"
                     /translation="MLSRVADSIYWMSRYVERAENVARFIDVNLNLMLDAPSGDDQQW
                     QPLVNITGDHETFTKRYSGGSTQKNVIQFLTFDRENSNSILSCLRAARENARSVREII
                     SSEMWLQLNTFYLMVNSAASSGKGFESPHEFFTEVKLSSHLFNGITDATMTRGEGWHF
                     SRLGRKLERADKTSRILDVKYFILLRSVADVGTPLDEVQWVAVLRSASAFEMYRKRHG
                     RISPKSIVEFLLLDPEFPRAIRFCLNAARDSLHAITGTPVGAYRKPVEKLLGQICSDL
                     AYANVDEIMKQGLHEYLDALQDKMNQASTGIYERFFAKRMPETHHSAPKERMQ"
     gene            4368..7748
                     /locus_tag="VG754_02930"
     CDS             4368..7748
                     /locus_tag="VG754_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWF18188.1"
                     /translation="MSIHVALNHKTHYRYDRPVAHSPHIIRLRPAPHCRTPILSYSQH
                     ILPEKHFLNWQQDPFSNFLARLAIPDKTNELLVEIDLVAEMAVFNPFDFFLEPAAEKF
                     PFQYEPWLKKELGPFLETAPPTPLLKEYLQTLDLTEGRTVDFLVQVNQRLWRDIKYLI
                     RLEPGVQEAETSLKKRCGSCRDSAWLFVQILRQVGLAARFVSGYLIQLTPDVKSLDGP
                     SGADVDFSDLHAWTEVYLPGAGWVGLDPTSGLFAGEGHIPLACSPEPTGAAPVTGSVD
                     KCESEFTHEMKVTRIHESPRVTKPYTEEQWQEIESLGHKIDSELKKNDVRLTMGGEPT
                     FISIDDMDGEEWNFTAVGPEKRRLSGELIRRMKRKFAPGGLLHYGQGKWYPGESLPRW
                     ALACYWRKDGQVIWQDDELFANEAVDYGHTEKEAEQFITQLARILEVKPKHVLPAYED
                     AWYYMWRERQLPTNVDPLQSRLEDKEERARLAKIFDQGLNKVAGYVLPLERQLTDRSR
                     WKSGPWFLRPEHLFLLPGDSPIGLRLPLDSIPWVSRSDYPYINPPDPMTELPPLPNRA
                     QLRKGQRAQFHPPQQFISETPGRTATSAARQLEESLRAEQNERAPARSQSADWIVRTA
                     LCVEPREGRLHIFMPPIRTSEDYLELISAIEETATELKLPVIIEGAAPPHDHRVDHIK
                     VTPDPGVIEVNLHPAHSWDELVDNTTTLYEEARQTRLGAEKFMIDGRHVGTGGGNHVV
                     IGGPTAADSPILRKPELLASLLRYWQNHPSLSFLFSGLFIGPTSQHPRVDEARNDSLY
                     ELELACKQIPEAADVPPWLVDRLFRDLLVDATGNTHRAEFCIDKLYSPDSSSGRLGLV
                     ELRAFEMPPHARMSLTQQLLLRSLIARFWQQPYEQKLVRWGTELHDRFMLPHFVEQDF
                     HDVIEDMQMAGYPLQPEWFAPHMEFRFPVCGTITQRGLVLEVRQALEPWHVLGEEGAS
                     GATVRYVDSSLERLQVKVRGMTDTRHQLACNGHAVPLHPTGTEGEYVAGIRFRAWQPP
                     QCLHPTIGVHAPLTFDLVDSWNQRSIGGCTYHVSHPGGLSYSKLPVNAYEAESRRLTR
                     FFKFGHTPGPMCLAEPERNPEFPFTLDLRCAPLPPRKE"
     gene            8068..>8449
                     /locus_tag="VG754_02935"
     CDS             8068..>8449
                     /locus_tag="VG754_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWF18189.1"
                     /translation="MSGSSDTEMLEQPAKMFAGYSALPGIYDEMALGPGELRPHWKTF
                     AASLDRLGRNEIALRWENARRIIREHGVTYNVYGDPQGMDRPWELDMLPLLVPPSEWA
                     KIEAGLVQRAKLFNFILTDLYGPKK"
BASE COUNT         1847 a         2498 c         2326 g         1778 t
ORIGIN      
        1 tagaattagg aaatcaggaa agcaggaaaa gacgatgttt cctcaagaat tttgttcctg
       61 atttcatgat ttcctaataa aaaactctcg atcttttttt ctaccgttca tctggtgacg
      121 acagccaaag attgacgctg caaccggact ccgggtaaaa ctacggcgca aatgagttct
      181 acatcaccag cgccgaccat cgcttatctc gcgcagggaa aagtccggtt gaaaatcgga
      241 accgcagcgc cgcgcacctt ggaaagcgct tacggcaatt ccatccgcga aaaagaaatc
      301 cgcgcgcaac aaaagcattc ctggaaatcc ggcggcggcg gttcgccgtt tggcggcccg
      361 gtgctttggg gcaagtcctc aatgcaacag gacgtgccgc ttgccatcac aagcatttgc
      421 ggcggcaagg aaaaaggcgg attgctctat tcaatggaaa gcggaagttt gtgcgcgctg
      481 ctggaagcgg gcgaattggg cgcggacgaa cgacgtttgt ggaacgacaa tcgcacgcgc
      541 atccggcacc tgagcatttc gcggcagacg ggcgatctgg cgttcggcgt cttgcacgag
      601 aacggcacgg ccaacatcgg cctgcgattg cacggcgaaa gcggcgtgaa ggaattgacc
      661 gagggcgatt cattcgacac cgcgccgcat tgggtccccg gcgaggggcg caaattggtg
      721 tttcaatccg ccggcatcgg ccgcaatcgc gagggacatt ttcttgcgct gggggcattc
      781 agcatccagc aattggacat tgaagcgggc gacatgacga cgttgctcga agagcagaaa
      841 tacgatcatc tcgcgccgca attcctcgaa gacggcaccc tgctctacat ccgccgtcct
      901 tacactttga atgaagggat gcatccgctg cgcacgatga aggatttttt cctgtttccg
      961 ttccggcttc tttttgccgt tttccaattc ctcaatttct tttccaaact tttcacgggc
     1021 cgccaactca cgaccgacgg cggcgccaag gcgcgcgaga tgaacatgcg ccagatgatg
     1081 atttggggaa acctcgtcaa tgcgcaacgt cccgcgcggc cggaggagga aggcgttgat
     1141 ctcattccca aatcatggga gttgcaccag cgcagtcccg acggcaaaac caaggtgctc
     1201 gccaccggcg ttttggccta cgatgtcgcg ccggatggat tgatcgttta cacgaacggc
     1261 aacgcgcttt ttctcctgca tccggacggg cgcaaggagc gtttgttcaa tgaatccatg
     1321 attgaacagg tcttttttgt gccggaataa aggccgtttt gcatgtccag ttttgaacat
     1381 cccgccaatt tctaaccagt ccgggcgaaa aaatcatgac ggatcgcaaa ggttgagcag
     1441 aacttcacaa agcagagtct taagttttgc ccggcaacga ttgcgcgcct gtcccattaa
     1501 caccgcggct ttggcccggt ttgcttgatg agacagagac ttgcagccgt ttcaacggct
     1561 ttccaatgga ttcgggtaag ccgttaaaac ggctggactg ctgttgatac gattacgcac
     1621 cgggctaaag ccacggtgtt aatgggaaaa actatatggc gctggagaca gccttggtgg
     1681 catttgaatt gctctttgcc tcgggagatt catgaattta agcgattacg agcttggcgg
     1741 cttttttgac gagatgtttg gcgagaacgg ccagccccgc gcggcaaccc gtccgcttgc
     1801 ccggaacatg cagtcgcttg ccgacgacga actcgtcaac cgccaactcg ccgccgaccg
     1861 cgcgctcgtc caggctggca tcactttcaa tgtttatggc gaaagtgccg gcgtcgaaaa
     1921 aacccttccc ttcgacctca tcccgcgcat cgtcacagcc acggaatggg aacgcatcga
     1981 gcgcggtctc aagcagcgca tccaggcgct gaatctgttc atcaacgatc tttatcacga
     2041 ccgcaaaatc atcaaggaca aggccgtgcc cgaggagatc atttcctcct cgaaaggatt
     2101 tcgcgagcaa tgcatcggca tgaatccgcc gcgcggcatc tggtgtcaca tcaccggcac
     2161 cgaccttgtc cgccatcgcg acgggcaggt ttacgttctc gaagacaacc tgcgttgtcc
     2221 ctccggcgtt tcctatgtgc tggaaaaccg gcgcttgatg aaaagtcttt ttcccgaagt
     2281 gttcgccgag tcgcacatca ggccggtttc aaattatccc atccgcttgc gcgacatgct
     2341 cgaacatctc tcgcccaacg gcgtcccgga accgcgcgtc gtgctgctca cgcccggtgt
     2401 ttacaattcc gcctatttcg aacattcgtt cctcgcgcaa caaatgggcg tggaacttgt
     2461 cgaaggcggc gacctcgtgg tgtcggaggg ctacgtctgg atgcggacaa cccacggctt
     2521 cgagcgggtg gacgtgattt accgccgcgt ggatgatgat tttcttgatc cggaaagttt
     2581 ccgcgcggat tcgatgctcg gcgttcccgg cttgatgcag gtttacaaat ccggcaatgt
     2641 cgccctcgcc aatgcgcccg gcaccggtgt cgcggacgat aaagtcgtct atgcctacgt
     2701 gccgcgaatc gtgaaatatt atttgggcga agaaatcatt ttgccgaacg tgccgaccta
     2761 tatttgcgcc gagcaaaagg accttaaata tgtgctggaa catctggatg aactcgtggt
     2821 gaaggccgca aatgaatcgg gcggttacgg catgttgatc gggccgcacg ccagcgccgc
     2881 cgaacgcgcc gagttcgcca aaaaaatcca ggagcatccc cgcaattaca tcgcgcaacc
     2941 gacgctggcg ctttcgcgcg tgccgacgct tgtggacggc cagcttgccg gtcgccacgt
     3001 ggacttgcgg ccttacatac tttatggaaa ggaaattttc gtgatgccgg gaggcttgac
     3061 gcgtgtcgcg ctcaagaagg gttcgctggt cgtgaattct tcgcaaggcg gcggttcgaa
     3121 agacacatgg gtgctcgcgg acaatcccag cgtggttgaa caggaccaaa aacaagatca
     3181 aaaacagagc gccacaagcg tggcgggagc agttgtctga tgctaagccg ggtcgccgat
     3241 tccatttact ggatgagccg ctacgtcgaa cgcgccgaga acgtggcgcg tttcattgac
     3301 gtcaatctca atctcatgct cgacgcgccg agcggcgatg accagcaatg gcagcctttg
     3361 gtgaacatca ccggcgacca tgagacgttc accaagcgtt attccggcgg ctccacgcag
     3421 aaaaacgtga tccaattcct cacgttcgac cgcgagaatt ccaattccat cctctcctgc
     3481 cttcgcgccg cccgcgaaaa cgcccgttcc gtccgcgaga tcatctcctc ggaaatgtgg
     3541 ctgcaactga acacgtttta tctgatggtc aattccgccg catccagcgg caaaggtttt
     3601 gaatcgcccc atgaattttt cacggaggtg aaactctcca gccatttgtt caacggcatc
     3661 accgacgcga cgatgacgcg gggcgaaggc tggcatttct cgcggcttgg ccggaaactg
     3721 gagcgcgccg acaaaacttc gcgcatcctg gatgtgaaat atttcatact gctccggtca
     3781 gtggcggatg tcggcacgcc gctggatgaa gtgcaatggg tcgcggtgtt gcgctcggcg
     3841 agcgcgttcg agatgtaccg caagcgccat ggccgcattt cgccgaagag catcgtcgag
     3901 tttttgctgc tggaccccga gtttccgcgc gccatccgtt tttgcctcaa tgccgcgcgc
     3961 gattcgctcc acgccatcac cggcacgccc gtcggcgcgt atcgcaagcc ggtggaaaaa
     4021 ttgctcggcc agatctgttc cgacctcgcc tacgcgaatg tggacgaaat catgaaacag
     4081 ggactgcacg aatatttgga cgctttgcaa gataaaatga accaggcgag cacgggaatt
     4141 tatgaacgat ttttcgccaa acgaatgcca gagacccatc acagtgcgcc gaaggaacgg
     4201 atgcaatgag cgcggtaaac accaaattcc aagcgccaag ctccagagaa taaccaaacc
     4261 ccaagctcga attcgcgttg tgcgaatttc gcagattggc tggttcaaca ggcgcctggc
     4321 aaattgaagt ttctcaggat gttggagctt tggtgcttta aaaaaacatg tccatacacg
     4381 tcgccctaaa tcataaaacg cactaccggt acgaccgacc ggtcgcgcat tcgccgcaca
     4441 tcatccgcct gcggcccgcg ccgcattgcc gcacgcccat tctcagctat tcgcagcaca
     4501 tcctgcccga gaagcatttt ctcaactggc aacaagaccc gttcagcaat tttctcgcgc
     4561 gcctcgccat ccccgacaaa acgaacgaat tgctcgtaga gattgatctc gtggcggaga
     4621 tggcggtttt caatccgttc gatttctttc tcgaacctgc cgcggaaaaa tttccgttcc
     4681 aatacgaacc gtggctgaag aaagaacttg ggccgttcct cgaaaccgcc cctcccacgc
     4741 cgttgctcaa ggaatatttg caaaccctcg atttgaccga ggggcgcacg gttgattttc
     4801 ttgtgcaggt caatcaacgc ctctggcgcg acatcaaata tctcatccgg ctcgaaccgg
     4861 gcgtgcagga agccgaaacc agtctcaaga aacgttgcgg ttcttgccgc gattccgcgt
     4921 ggttgttcgt ccagatactt cggcaagttg gtctcgccgc gcgatttgtt tccggctatc
     4981 tcatccagtt gacacccgac gtcaaatcgc tcgacggccc ttccggggcg gatgttgatt
     5041 tcagcgatct tcatgcctgg acggaagtct atctgcccgg cgcgggttgg gtcgggttgg
     5101 atccgacgtc cggtttgttc gcgggcgaag gccatattcc cctcgcctgt tcgccggaac
     5161 cgaccggcgc tgcgcctgtc accggttccg tggacaaatg cgaaagcgaa ttcacccacg
     5221 aaatgaaagt gacgcgcatc cacgaatcgc cgcgtgtcac caagccttac accgaggagc
     5281 aatggcagga aatcgaatcg ctcggccaca agattgacag cgaattgaaa aagaacgacg
     5341 tgcgcctcac gatgggcggt gaaccaacgt tcatttcaat agatgacatg gatggcgagg
     5401 aatggaattt caccgcggtt ggccccgaaa aacgccgctt gtccggcgaa ctcatccgcc
     5461 gcatgaaaag gaagttcgcg cccggcggtt tgttgcatta cggccagggc aaatggtatc
     5521 ccggcgaatc attgccgcgc tgggcattgg cttgttattg gcgcaaggac ggccaagtca
     5581 tctggcagga tgatgagttg ttcgcgaatg aagccgtcga ttacggccat accgaaaagg
     5641 aagcggaaca attcatcacg caactggcgc gcattttgga agtcaaaccc aagcacgtcc
     5701 ttccggcata cgaagacgcg tggtattaca tgtggcgcga acgccagctt cccacgaatg
     5761 ttgacccgct ccagtcccgt ctcgaagaca aggaggaacg cgcgcgcctc gcgaagattt
     5821 tcgatcaagg cttgaacaag gtcgccggtt acgttctgcc gctggaacgg caactcaccg
     5881 accgctcgcg ctggaaaagc ggcccgtggt ttttgcgtcc ggaacatctt tttctgctgc
     5941 cgggcgattc ccccatcggc ctgcgcttgc cgctcgattc gattccgtgg gtttcgcgct
     6001 ccgattatcc gtacatcaat ccgcccgacc cgatgaccga attgccgccg ctgcccaatc
     6061 gcgcccagct tcgcaaagga cagcgcgcgc aattccatcc accccaacaa ttcatttcgg
     6121 aaacgccggg acgaaccgca accagcgccg cgcgccagtt ggaagaatcg ttgcgcgccg
     6181 agcaaaacga acgcgcgccc gctcgctcgc aatccgccga ctggattgtc cgcaccgcgc
     6241 tctgtgtcga gccgcgcgag ggacgcctgc acattttcat gccgcccatc cgcacttcgg
     6301 aagattacct ggaactgatc agcgccatcg aggaaaccgc gaccgaactc aagctccctg
     6361 tcatcatcga aggcgccgcg ccgccgcacg atcaccgggt tgatcacatc aaggtcacgc
     6421 ccgaccccgg cgtcatcgag gtgaacctgc atccggcgca cagttgggat gaattggtgg
     6481 acaacaccac cacgctttac gaggaagccc gccaaactcg cctcggcgcg gaaaaattca
     6541 tgattgacgg acgccacgtt ggcactggcg gcggcaacca tgttgtcatc ggcgggccaa
     6601 cggcggcgga cagtcccatc ttgcgcaaac ccgaattgct cgcgagcctc ttgcgctact
     6661 ggcaaaacca tccgtcgctt tcgtttttgt tcagcgggct gttcatcggc ccgacgagcc
     6721 agcatccgcg cgttgacgaa gcgcgcaacg attcgcttta cgaactggaa cttgcctgca
     6781 aacaaatccc cgaagccgcg gacgtcccgc catggctcgt ggaccggctg ttccgcgatc
     6841 tgctcgtgga cgccaccggc aacacgcatc gcgccgagtt ttgcatcgat aaattgtatt
     6901 cgcccgattc gagcagcgga cggctcgggt tggtcgagtt gcgcgcgttc gagatgccgc
     6961 cgcacgcgcg gatgagcctc acgcaacaac tcctcctccg ctcgttgatc gcgcgtttct
     7021 ggcagcaacc ttacgaacaa aagctcgtcc gctggggaac ggagcttcac gatcgcttca
     7081 tgctgccgca ttttgtcgag caggattttc acgatgtcat cgaagacatg caaatggccg
     7141 gttatccgct ccagcccgaa tggttcgcgc cgcacatgga atttcgcttt ccagtttgcg
     7201 gcacgattac gcaacgcggc ctggtgttgg aagttcgtca agcccttgaa ccgtggcacg
     7261 ttctcggcga ggaaggggct tccggcgcaa cggtgcgtta cgtggattca tcgctcgaac
     7321 gattgcaggt caaggtgcgc ggcatgaccg atacgcgcca tcaactcgcc tgcaatggcc
     7381 atgcggtgcc gctgcatccg accggcacgg aaggcgaata tgtcgcgggc attcgttttc
     7441 gcgcctggca accgccgcaa tgtttgcacc cgaccatcgg cgttcatgct ccgctgactt
     7501 tcgacctcgt ggacagttgg aaccaacgtt ccatcggcgg ttgcacttat catgtttcgc
     7561 atccgggcgg gcttagttac agcaagctgc cggtcaatgc ctacgaagcc gaaagtcgcc
     7621 gccttacgcg gttttttaaa ttcggccaca cccccggccc aatgtgcctt gcggaaccgg
     7681 agcgcaatcc cgaatttcca ttcacgctcg atttgcgttg cgccccattg ccgcctcgca
     7741 aagaatgact caatttagtg tcatgacgat gcaagaaaat atttcaaagc atgtgggtgg
     7801 gattggcccg ctcggttcgc gtgcgccgct attcccctca cccctgccct ctctcttggg
     7861 gagagggaga atcgtatcca gccatggttg caaagttgcg gtaacagatt tgccgaaaga
     7921 gagaagaatg gttcaccctc tccttgggga gagggccggg gtgaggggga acgaaacgtc
     7981 aagttctgca atcggcacgg ccatctatat aagtgccggc caatccttcc ccactccgca
     8041 ctccgcactc cgcattcccc actccccatg agcggctctt cagacacaga gatgctcgaa
     8101 cagcctgcga aaatgtttgc aggctattcg gcgttgccgg gcatttacga tgagatggcg
     8161 ctcgggccgg gcgaattgcg gccgcattgg aaaactttcg cggcgtcgct cgaccggttg
     8221 ggccgcaacg aaatcgccct gcgctgggaa aacgcgcggc gaatcatccg cgaacacggc
     8281 gtcacttaca atgtctatgg cgacccgcag gggatggacc ggccctggga attggacatg
     8341 ttgccgctgc tcgtgccgcc ctccgaatgg gcaaagatcg aggccggact tgttcaacgc
     8401 gcaaaattgt tcaacttcat cctcaccgac ctttacggtc cgaaaaaat
//
LOCUS       DASZMM010000117         4513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1420190, whole genome shotgun sequence.
ACCESSION   DASZMM010000117 DASZMM010000000
VERSION     DASZMM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420190"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VG754_02940"
     CDS             <1..402
                     /locus_tag="VG754_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWF18190.1"
                     /translation="GKARIARPGRDLTIVAYSAMVHEALAVAEELVTDGIEVEVVDLR
                     SVKPLDTDTVMASVARTGRLLCVGESWPWGGVTAEVIARVASEGFGLLDAPPQRLNAK
                     DTPVPYHPNLWAVHRPTARSIASAARNLVRL"
     gene            407..1612
                     /locus_tag="VG754_02945"
     CDS             407..1612
                     /locus_tag="VG754_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWF18191.1"
                     /translation="MPQIPIIMPQLGESIAEATIVRLLVKAGDKVEADQDVLEVETNK
                     ATMNVSSPCPGRVERLLVKLDESYPVGAVLGYLEASAEDVARLGLEQPESPNGHSETS
                     NGDAETKPASEIRQRGVQPTVRGLPVPANATGASYMSPRMKARMAELGLHAADLAGIA
                     GSGAAGRLTIQDFEKFLVNLERNQLSQASSMRVAVADAMRRSWTRPLATVALPVCLDP
                     MLAHRKTCDPKPGPALYAMRALALALAENSAPAGRLVGNKIVHPTSIDVGFAVEAEDG
                     VLVPVIRNTDKKQLKEVVGRYNELVELARQRRLPPDTTGGSIATVTNFGTFGLTWATP
                     IPLPEQTLVLGMGAGRLVPHWDPAKGQFVPIMEANLTLSFDHRVLDGGAAGRLLARIA
                     ALLAKPENL"
     gene            2152..3666
                     /locus_tag="VG754_02950"
     CDS             2152..3666
                     /locus_tag="VG754_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18192.1"
                     /translation="MIFGKTRVVWPMIGALLFFGVLWLLDFPKPSYDDLFQAGAAFHM
                     AEGGDFSNPLIERQEFPSHYFFVYPPLNSYLLAGWLKVFGINAASVTAYPICSCMVIC
                     VATILVLRRHQASVWLQWFVPLGVSFGILYMGLRPEPMAVALAMTGFAITDSEAGRKN
                     GVIQWLGFFLMIMGASAAPRVTLFVGALVVYALYRGWRDKATKQERWNVVFRWLGAGA
                     ATGLLFLVMIGFRVAEFLHTFHFHAAGRISNDKVWVIKQYLFGYLGYLQLTILLMPLV
                     LLAWVLRKPKDELSRPALWVAAMAPVAFCTGGIGSGTAWWAFLVMILLAGSLVKTLSR
                     KGAIALQVCIFLILVVINRKVAAECWGIVSGHITRDQGQQMAEAKAIQPTPEHPVLLD
                     GWVARYLYDYRLPKGVIDAEWSVRFPGAGPGSYATSGDAGPQLRKGDIFVVCNYMRHS
                     LQTYTLLEKTTDPLWRAFGLKQLAFDPYPRVVYIIPAEDCKDVRPGAAQRVPGS"
     gene            complement(3737..>4513)
                     /locus_tag="VG754_02955"
     CDS             complement(3737..>4513)
                     /locus_tag="VG754_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18193.1"
                     /translation="IINWLFLGRRGALACVALLAAVFLGQAKGPCRRYLLCWSCVGIF
                     AAGPNTMAYIKIMGLPNNLCLFEIWMFLLVWPVIVMFPQLFPQLISVQTSTPTGWKPR
                     AITAVLYLGVIVYAVALLPLKRIPSASEYDFGREVEAGVRRDLQAGRKVLVSHGAACL
                     VHCGITEPPLDRCNSALEIFTGNKPELLSGLKARIAAHHYDRIYLTLGSYYGPDVLDE
                     IHKNYEVETVIPKIRPPKGFFTLRGAVMKECTEYAVLKPK"
BASE COUNT          927 a         1268 c         1290 g         1028 t
ORIGIN      
        1 ggcaaggcgc gcatcgcacg gcccggacgc gacctgacca tcgtcgctta cagcgcaatg
       61 gtccacgagg cgctcgccgt tgccgaagaa ttggttaccg acggcatcga agttgaagtg
      121 gtggacttgc gttccgtgaa accgctcgac accgataccg tcatggcatc cgttgcgcgc
      181 accggacgat tattgtgcgt cggcgaatca tggccttggg gcggcgtcac tgcggaagtc
      241 atcgcgcgcg ttgccagcga aggttttggt ttgctcgacg cgccgccgca acgcctcaac
      301 gccaaggaca cacctgttcc ttatcatcca aacttgtggg cggtgcatcg gcccacggcg
      361 cgaagcatcg cctccgccgc gcgcaattta gttcgcctct gaccctatgc cacagattcc
      421 catcatcatg ccgcaactcg gcgaatccat cgccgaagcc accatagtgc gcctgctcgt
      481 caaggccggc gataaagtcg aggccgacca ggatgtgctc gaagtcgaga ccaacaaggc
      541 caccatgaat gtttcttcgc cttgtcccgg acgcgttgaa cggctgttgg tcaagctcga
      601 cgaaagctat cccgtgggcg cagtgctcgg ttatctcgaa gccagcgctg aagacgtcgc
      661 ccgtctcggc cttgaacaac ccgaaagtcc aaacggccat tccgaaactt ccaatggcga
      721 tgcggaaacc aaaccagctt ccgagattag acaacgcggc gttcaaccta ccgttcgcgg
      781 tctgcccgtt ccggccaatg ccacaggcgc gagttacatg tccccgcgca tgaaggcccg
      841 catggcggaa ctcggattgc acgcggcgga tttggccggc atcgccggca gcggcgcggc
      901 gggacgtttg accatccagg attttgagaa atttcttgtc aaccttgagc gcaaccagtt
      961 gagccaggcc tccagcatgc gcgtggctgt cgcggacgcc atgcggcgca gttggacgcg
     1021 cccgcttgcg accgtcgcct tgcccgtctg ccttgacccg atgctcgccc atcgcaaaac
     1081 ctgcgatccc aagcccggcc ccgcgcttta cgccatgcgc gcgcttgcgc ttgcgctcgc
     1141 ggagaacagc gcgcccgccg gacgattggt tggcaacaag atcgtgcatc ccacctccat
     1201 tgacgtcggc tttgccgtcg aggccgaaga cggcgtgctc gtgcccgtca tccgcaacac
     1261 tgacaagaaa cagttgaagg aagtcgttgg tcgttacaac gaactcgtcg agctcgcgcg
     1321 tcaacgccgt ttgccacccg ataccactgg cggttccatc gccactgtca ccaatttcgg
     1381 caccttcggc ctcacctggg ccacgccgat cccgttgccc gaacaaacgc tggtgctcgg
     1441 catgggcgca ggccgtctcg ttccgcactg ggacccggcg aaaggccaat tcgtccccat
     1501 catggaagca aacttgaccc tgagcttcga ccaccgcgtc ctcgacggcg gcgcagccgg
     1561 aagattgctg gcgcggatcg cggcattgct tgcaaagccg gaaaatctct aagcaggcaa
     1621 gacatgaatt ttcacaaatt aaaccggcgc tgttcccatt tgtgaaattc gtgtaattcg
     1681 tgtctttctt tggagctttt aagtcttgcc tcctcaaacg atggcctggt gatgaggacc
     1741 gttcggcaac ggaggtctgc tcaaagaaat gcctgacgag gatttcaaat cttttagatc
     1801 gcctccatac aaatagacaa cgtgctccgt ccgtgaaata atcagcctcg cgtgaatcaa
     1861 ggagcgcaac ataggacgcc gtccggtgtc cacgataaat ggccattcgc caaatcccga
     1921 gaattttggt ttccaatctc ctttggagga gccgacgcga gaacgctcgc attcgatttg
     1981 taatttcaaa tctcaagtag gtaattccaa atttacactg gcccgcgacg gaatggctgt
     2041 ttgtttgtca aaagagcttg ccttcccgca gcggtttgaa tacggcttag gtttggtccc
     2101 gtcgccgcga gaatccactt gcgttgaacg acgggcaaca cttggtcgct tatgattttt
     2161 ggcaaaacgc gggtggtctg gccgatgatt ggcgcgctgc tcttctttgg cgtcttgtgg
     2221 ttgctggatt ttcccaagcc gtcctacgac gatttgttcc aagcgggcgc ggcgtttcac
     2281 atggccgagg gcggggattt ctcgaatccg ctgatcgaac ggcaggaatt tccgagtcat
     2341 tacttctttg tctatccgcc gctcaattcg tacctgctcg ccggatggct caaagtgttc
     2401 ggaatcaatg cggcttcggt caccgcttac ccgatatgca gttgcatggt gatttgcgtg
     2461 gcgaccattt tggttctgcg ccgccatcag gcttcggtat ggttgcaatg gtttgtgccg
     2521 ctgggtgtgt cgttcgggat tctgtacatg ggccttcgtc ccgagccgat ggctgtcgcg
     2581 ctggcgatga ccggttttgc catcacggat tccgaagcgg gtcgaaaaaa tggagtgatc
     2641 caatggctcg gtttttttct gatgatcatg ggcgcatccg ccgcgccacg agtgacattg
     2701 tttgtgggag cgctggtggt gtacgcgctt tatcgcggat ggcgggacaa agccacaaag
     2761 caggaacgtt ggaatgtcgt gttccggtgg cttggcgcag gcgcggcaac gggtctgctg
     2821 tttttggtta tgatcggttt ccgcgtcgcc gaatttctcc acactttcca tttccacgcc
     2881 gccggacgca tcagcaacga caaggtttgg gtcatcaaac aatatttgtt cgggtacctc
     2941 ggttatttgc aattgacgat actgttgatg ccgctcgtgc ttttggcttg ggtgctcaga
     3001 aagccaaaag acgaattgag ccgtcctgct ttgtgggtcg ctgcgatggc cccggtcgca
     3061 ttttgcaccg gaggcattgg gtcgggaacg gcttggtggg cttttttggt gatgattctc
     3121 ctggccggtt cgctcgtgaa aactttgtcg aggaagggag caattgctct gcaggtctgc
     3181 atctttttga ttctcgtggt gataaaccgc aaagtcgccg cggaatgctg gggcattgtc
     3241 agcggtcaca tcacgcgcga ccaaggtcaa caaatggccg aagcaaaggc catccagccc
     3301 acgccggaac atccggtttt gttggacggt tgggtggcgc ggtatcttta tgattatcgg
     3361 ctgcccaaag gtgtcattga cgcggaatgg agcgtgcgtt ttcccggcgc ggggccaggc
     3421 tcgtatgcga cttccggtga tgcgggtccg caacttcgga agggagatat tttcgtggtt
     3481 tgcaattaca tgcggcattc gcttcagact tacacgttgc tggaaaaaac gaccgatcca
     3541 ctttggcggg cattcgggtt gaagcagctt gcttttgatc cttatccgcg tgtcgtgtac
     3601 atcattccag ccgaggattg caaagatgtc cgtcctggcg cagcccaacg cgtgcccggc
     3661 agctaaccaa gaacaatgcg cttccttcag gtcgtttttg gcgacatgcc gcgaacgagc
     3721 ttttctggcg aggatgttat ttgggtttga gaacggcata ttccgtgcat tccttcatga
     3781 ccgcgccgcg aagggtgaaa aagcctttcg gagggcgaat ttttgggatt acggtttcga
     3841 cttcataatt cttgtgaatc tcatcaagca catcgggacc gtaatagctg cccagcgtca
     3901 ggtaaatccg gtcataatga tgagcagcaa ttcttgcctt caggccggac agcaactcgg
     3961 gtttgttgcc cgtaaagatt tcgagcgcgg aattgcaccg gtcgagaggt ggttcggtga
     4021 ttccgcaatg gacgaggcag gccgctccat gcgagacaag caccttgcgg ccggcttgaa
     4081 ggtcgcgtcg cacgcccgcc tcaacttcgc gcccaaaatc atattccgac gcggacggaa
     4141 ttcgcttcaa tggaagaagc gcaacggcat acacaatcac gccaaggtag agcacagcag
     4201 tgatggcgcg aggtttccat ccagtgggtg tggatgtttg aacactgatg agttgcggga
     4261 agagttgtgg aaacatcacg atgaccggcc aaacaagcaa gaacatccag atttcgaaga
     4321 ggcaaagatt attcggcagg cccatgatct tgatgtaagc cattgtgttc gggccggccg
     4381 caaaaatccc aacgcaggac caacagagca agtaacgccg gcacgggcct tttgcttgcc
     4441 ccaaaaaaac cgctgccaga agcgccacgc aggcaagcgc gcctcttctt cccagaaaca
     4501 gccagttgat gat
//
LOCUS       DASZMM010000118         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3344432, whole genome shotgun sequence.
ACCESSION   DASZMM010000118 DASZMM010000000
VERSION     DASZMM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3344432"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..954
                     /locus_tag="VG754_02960"
     CDS             85..954
                     /locus_tag="VG754_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18194.1"
                     /translation="MKTLFLTLALLTALAGSVSAQVTVEVLMGQDQFVANESIPVKVR
                     LVNHSGQTLLFGQEDWLSYSVEARDGLVVLKSGEPPQPHDFDVKSAEVATTPRTDLAP
                     YFTIAQPGRYSVTATVRIKDWDKAVVSAPVTFEVVRGMRIWEQDFGVPQSATNHNQPE
                     VRKYILQQATMSRCMKLYFRLTDAAETKTLRVAPIGPLISFSDPQTRVDQDSNLHLLY
                     QEGAHVYNYMVFNPDGDMTVRQTYAYTDSAPHLKVDNTGKVLIFGGARHLAENDIPTS
                     RPSLTNVIPPPVR"
     gene            929..1960
                     /locus_tag="VG754_02965"
     CDS             929..1960
                     /locus_tag="VG754_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HWF18195.1"
                     /translation="MSFRRPFVNLICRILAFAVLFNGALAQGQNVILHLRNGDRVTGI
                     IVSETTNQLVLSNVWVKELTVPLAQIERRESLPIAIASGGTNATTNLTSTNLLTSTNR
                     LLGTNVLAGKLPVNTNTFWRRWSGTAAVGMDLEKGAANHELYYGKANLTYAQPYESDP
                     KEFFRNILTYDAAYGKTSGVLSDNRMGGSSKTDFDLSRRIYVYNLGAAFYDEIQKINL
                     HYEDGPGLGYHWFNHTNLAVNLELGANYQVEDRVGGTNTYSAYYRIGQDLTWKMNKQF
                     SLTEKFEYFPRATAPSQYRMRFESTLTYALLLHLSYNLTVADYYDTQPTLGVPNNDLQ
                     IRTSLGVKF"
     gene            complement(2050..2589)
                     /locus_tag="VG754_02970"
     CDS             complement(2050..2589)
                     /locus_tag="VG754_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HWF18196.1"
                     /translation="MKKAIFLERDGILNLVKVEKQAQAGPLTLEEFQLNPAALPVCEH
                     LKAAGFLLIATTNQPGVSRGTISRRELDRMHAFLRQSLPLDDLLVCPHDVDDGCSCRK
                     PQPGLLMEAAFKWHIDLERSFVISDKWQDAKAARAVGCTSMLLQSPWIGTGHHDFLLS
                     SLPAITGKILQLQARHLAA"
     gene            2917..4287
                     /locus_tag="VG754_02975"
     CDS             2917..4287
                     /locus_tag="VG754_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HWF18197.1"
                     /translation="MLNYIWLGLIMAAVLMAGSNGQIPEMTKKAFDMCNTAVMEIGLP
                     LVGVMALWLGIMRLAEKSGMVQLLARLLRPVMVRLFPDVPAEHPAMGSMLMNMAANML
                     GLANAATPLGIRAMEDLETLNPRPGTATNAMCTFLAINTSSIQLIPVTVVGVLAVKGS
                     TNPSAIIGTSVMATTVAFIAAIVTVKSLEKLPMFHLPPITPGEVHEKKAEVKHEKETE
                     GEPAAIKPLQWWGHLALALFFLFFAGLLTLWMFYPNTQAFANSIGLPNLFKTIPFDPA
                     DGMLTRIVKCVSALSIPLLLSFFPLYAFLRGIKVYEEFVEGAKEGFQIIIRIIPNLVA
                     ILVAIAMFRASGGIDMLTGWLKPMMNALRFPAELLPMAIIRPLSGSGSFGVFTELVQK
                     YGPDHLFSRTAGTIYGSTETTFYVLAVYFGAIGIKRARHAVPAGLVADIVAILASVVI
                     CKMVFA"
     gene            4369..>4656
                     /locus_tag="VG754_02980"
     CDS             4369..>4656
                     /locus_tag="VG754_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HWF18198.1"
                     /translation="MSLRDTATEMVRGLQTSGHSAYWVGGCVRDFLLGREPQDYDIAT
                     SAVPDQIEALFPRTIPVGRKFGVIIVLEDGHEFQVATFRAEADYQDGRHPQQ"
BASE COUNT         1028 a         1292 c         1243 g         1093 t
ORIGIN      
        1 ggcataattt ttgaatcgcg actgcaagca aaaccatggt tttgctgctt gcttgtcgtg
       61 gctgcggctc attaaagtga caggatgaag acattatttt taacgctcgc gctgttgacc
      121 gcgttggcgg gaagtgtttc cgcgcaggtc acggtcgagg tgttgatggg ccaggaccaa
      181 ttcgtggcca atgaatccat tccagtcaag gtccgcctcg tcaatcactc cgggcagacg
      241 cttttgtttg gccaggagga ctggctttcc tattcagttg aagcgcgcga cggattggtg
      301 gtgctcaagt ccggcgagcc gccgcagccg catgattttg atgtgaagtc cgccgaggtg
      361 gccaccaccc cccgcacgga ccttgcgccg tatttcacca tcgcgcagcc cggccgttat
      421 tcagtgacgg ccacggtgcg catcaaggat tgggacaagg cggtggtcag cgcgccggtg
      481 accttcgagg tggtgcgcgg catgcgcatt tgggagcagg atttcggggt tccacaatct
      541 gccaccaatc acaaccagcc cgaggtgcgc aaatatatcc tccagcaagc gaccatgtcg
      601 cgctgcatga aactttattt ccggctcacc gatgccgcgg aaacgaagac gctccgagtt
      661 gcgcccattg gcccgctgat ttctttcagc gatccgcaaa cgcgggtgga ccaggacagc
      721 aatctgcacc tgctttatca agaaggcgcg catgtctata attacatggt ttttaatccc
      781 gacggcgaca tgaccgtgcg ccagacttat gcctacaccg attcggcccc gcacctgaag
      841 gtggacaaca ccggcaaagt cctgattttt ggcggcgcgc gtcatcttgc ggaaaatgat
      901 atcccaacca gccggccttc cctcaccaat gtcattccgc cgcccgttcg ttaacctgat
      961 ttgccgcatt cttgcgtttg ccgtgctttt caacggagcg cttgcgcagg gacaaaatgt
     1021 catccttcat cttcgcaacg gtgaccgcgt caccggcatc atcgtctctg aaaccacgaa
     1081 ccagttggta ttgtccaatg tttgggtcaa ggaattgacg gtgccgttgg cgcagattga
     1141 aaggcgcgaa tcgcttccaa tcgcgattgc atccggcggc acaaatgcca ccaccaatct
     1201 gacgagcaca aatcttttga cgagcacaaa tcgtttgctt ggcacaaatg ttcttgcggg
     1261 caaacttccc gtgaacacaa acaccttttg gaggcgttgg tcgggcactg cggcagtcgg
     1321 catggatttg gagaaaggcg cggctaatca cgagctttat tatgggaagg ccaatctcac
     1381 ctacgcgcag ccttatgaaa gcgaccccaa ggaatttttc cgcaatatcc tgacgtatga
     1441 cgccgcctac ggcaaaactt ccggcgtcct ttccgacaac cgaatgggag gaagttcgaa
     1501 aacggatttt gacctgtccc gcaggattta cgtgtacaat ttgggcgctg ccttttatga
     1561 cgagatccaa aagattaatc tgcattacga agacggcccc ggcctgggtt atcattggtt
     1621 caaccatacg aacctggcgg tgaacctgga attgggcgcg aattatcagg tcgaagatcg
     1681 tgtcggcggc acgaatacat acagcgctta ctaccggatt gggcaggacc tgacatggaa
     1741 aatgaacaag caattcagcc tgacagagaa attcgagtat ttcccgcgcg ccactgctcc
     1801 ttcacagtat cgcatgcgct ttgagtccac cttgacctac gcattgttgc tccatctttc
     1861 ctataatctg acggtcgcgg actactacga cactcagccc acactcggcg ttcccaacaa
     1921 tgatttgcag atccgcactt cgctcggggt gaaattttag gaacatcgcc cggcgttgtt
     1981 gcgcccgccc catggcagtg ggacaaatca aattcgggcg ggcgcaacta atttggctgg
     2041 gcattttgtt caggccgcga gatgacgcgc ttgcaattgc aggatttttc ccgtgatcgc
     2101 cggcaggctt gaaagcagaa aatcatggtg ccccgttcca atccagggcg attgcaacaa
     2161 cattgaagtg caaccgacgg cgcgcgccgc cttggcgtcc tgccatttgt cgctgatgac
     2221 aaatgaccgt tccaaatcga tgtgccattt gaaggccgct tccatcagca ggccgggctg
     2281 tggtttgcgg caggaacagc cgtcgtccac gtcatgagga cagaccagga gatcatccag
     2341 tggaaggctt tggcgaagaa atgcgtgcat ccggtccaat tcgcggcgcg aaatcgtgcc
     2401 gcgcgaaacg cccggctggt ttgtcgtggc gatcagaaga aaaccagcgg ccttgaggtg
     2461 ttcgcacacc ggcagcgcgg cgggattgag ttggaactct tccaaggtca gcggacccgc
     2521 ctgggcctgc ttctcgacct tcaccagatt cagaatccca tctcgttcca aaaaaatcgc
     2581 ttttttcata ctccaaatac gagatagcat aaagctcgcc tgatttgacc gagggtcaaa
     2641 aaaacccgca gaacctcaaa aaccggccat ttttgggcct ttctcattgc aattttgaga
     2701 cgagcggcct gcgaattgag cagcaaggtc aattatctcc attttgaact gtcatttgca
     2761 atacattcca atgattaaga ggacaaaatg catcagaatg ttccggggtt aatgtccata
     2821 aatacaatcg cagggaattg tgtttagagg atgtgacatg tcacggaccc gatttttctc
     2881 gcgtaatttc caggccggac gtatagccat ggccccatgt tgaattacat ctggcttggc
     2941 ttgatcatgg ccgcagttct tatggccggc agcaacggcc aaatcccgga gatgaccaag
     3001 aaggcgttcg acatgtgcaa caccgcggtc atggaaatcg gcctgccgct agtgggtgtc
     3061 atggcgctgt ggctcggcat catgcgattg gcggaaaaat ccggcatggt gcaactgctt
     3121 gcgcgtttgt tgcggcccgt catggtccgg ctttttcccg atgtccccgc ggaacatccc
     3181 gcgatggggt ccatgctgat gaacatggct gccaacatgc tcggccttgc caacgcggca
     3241 acacctctcg gcatccgcgc gatggaggat ttggaaacgc tcaatccacg tcctggcacc
     3301 gcaacgaacg ccatgtgcac tttcctcgcc atcaacacga gttcgatcca attgattcct
     3361 gtgacggttg ttggcgttct cgcggtcaaa ggctccacaa atccttccgc aatcattggc
     3421 acttccgtca tggcaacgac tgtcgcattc attgccgcca tcgtcacagt caagtcgctt
     3481 gaaaaacttc cgatgttcca cctgccgccg atcacgcccg gcgaagttca cgaaaaaaaa
     3541 gccgaggtta aacacgaaaa ggaaaccgaa ggcgaacctg cggcgatcaa accgttgcaa
     3601 tggtggggtc acttggcgtt ggcgctgttt tttctgtttt ttgccggttt gttgacatta
     3661 tggatgttct atccaaacac acaggcgttt gcaaactcga ttggtttgcc gaatttattc
     3721 aaaacaatcc catttgatcc cgccgatgga atgttgacgc gcatcgtcaa atgcgtctcc
     3781 gcgctttcca tcccgttgct gctctccttt tttccgcttt acgcgttttt gcggggcatc
     3841 aaggtgtacg aagaatttgt tgaaggcgcg aaggaaggat ttcaaatcat cattcgcatc
     3901 atcccgaacc tcgtggccat cctggtggcc atcgcgatgt tccgcgcttc cggcggaatt
     3961 gacatgctga cgggttggct caaacccatg atgaatgcgt tgcgtttccc ggcggaattg
     4021 ctgccgatgg cgatcatccg tccattgagc ggcagcggga gcttcggagt gttcaccgaa
     4081 ctagtccaga aatatggacc ggaccacctc ttcagccgaa cggctggaac catttatggc
     4141 agcactgaga cgacgttcta tgtgcttgcg gtttatttcg gcgcaattgg catcaagcgg
     4201 gcgcgacatg ccgttcccgc gggattggtt gcggacatcg tcgccatcct cgcctcggtc
     4261 gtcatttgca aaatggtgtt cgcctagcaa agagatttcg cgcgaaggcc gcgaagcaga
     4321 aaataccttc gccttcttcg cgcgacaatt ttgctatata ttgattttat gtccttgcgc
     4381 gacacagcca cagaaatggt ccgcggcctc cagacgtccg gtcattctgc gtactgggtc
     4441 ggcggttgcg tgcgtgattt cttgctcgga cgcgaaccgc aggactacga catcgccact
     4501 tccgccgtgc cggaccagat cgaggcgctt tttccgcgaa caattcccgt cgggcgcaaa
     4561 ttcggcgtca tcatcgtgct ggaggatggc catgaatttc aggtcgccac gtttcgcgcg
     4621 gaggcggatt accaggatgg ccggcatccg caacag
//
LOCUS       DASZMM010000119         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4174198, whole genome shotgun sequence.
ACCESSION   DASZMM010000119 DASZMM010000000
VERSION     DASZMM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4174198"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VG754_02985"
     CDS             complement(<1..459)
                     /locus_tag="VG754_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF18199.1"
                     /translation="MAERMISDVLIKPDAALEMTLRPSLFSEFTGQPKVKERLEIAVT
                     AARQRSEAIDHILLSGPPGLGKTTLANIIAKAMGANLKSTSGPTIEKAGDLAGLLTNL
                     EEGDVLFIDEIHRLQKTIEEYLYPAMEDFKLDIIIDQGPNARSVRLNLPRF"
     gene            574..1083
                     /locus_tag="VG754_02990"
     CDS             574..1083
                     /locus_tag="VG754_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18200.1"
                     /translation="MSGDPQAGTRATSNKFLFPPWLWPVLVSGLAWAVIFLAIPPDRQ
                     DFPLGDDWAFSHSAIWFAHGLGIHYVKWASMPQLGQWLWSWPFLHIIHWPHVALRLSA
                     IVLSWLGLAAFCDLLRLENVPERVAAFAACVLALDPLFFISQGDLHDGCAGAVVWVDR
                     AGFLFAGSG"
     gene            1016..2227
                     /locus_tag="VG754_02995"
     CDS             1016..2227
                     /locus_tag="VG754_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18201.1"
                     /translation="MTDVPALSFGLIALAFYSRALAEKRLRWLPAATLFAVFAVITRQ
                     TMLMIPLAAAVPMLRNQEMRRSAAWGLSILVPLAVCVYASRWFSQRPDVEPMHAKFNL
                     ESMAFRPFVALHLAGLVVLPLIFLAGRQRSWRVFFAAFTAMLLAAGFYHSLAARLPYG
                     GWFPYCMGMLSLEGTYPDGLVVGQRDILLTPTVQVELTVLGCAGAALVLAALWEMIRA
                     RKFPGALLVFTSLQFCILLIMPVMADRYLEVLFPGAIFLAATCCSMAGIGRLAGIVAV
                     VVSGFIAVALCHDCFAWNSARWELGREAIAAKRIHPADIEGGFEWNGWYERNDPAWAP
                     VSNVSPVADKDPASLTLRFSRIYFPTVTGQYALAFSQPSNTIIIATMPYSTWLPPAKR
                     DFFLVQQKPQR"
     gene            2635..>3693
                     /locus_tag="VG754_03000"
     CDS             2635..>3693
                     /locus_tag="VG754_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWF18202.1"
                     /translation="MYKIKSWFWILLALLGTSTGAFASETDINIPDLSGVSFPGLGGV
                     SGPTLMYIGIAVCVVGALFGLLQYTQTKALPVHSSMANVSNTIWETCKTYLFTQGKFL
                     MILWVLIAACMIYYFGHLQQNTASHVAVILLASILGILGSYGVAWFGIRINTVSNSRT
                     AFSALKGNPLATLGIPLRSGMSVGLLLVAVELFFMICILMFLPRELVGPCFIGFAIGE
                     SLGASVLRICGGIFTKIADIGSDLMKIVFKLPEDDPKNPGVIADCTGDNAGDSVGPTA
                     DGFETYGVTGVALIAFLALALHAAESQAICAKLIIWLFTMRALMIVTSLVSYFANEML
                     SKTMFGGKKDFDFEAPLTH"
BASE COUNT          702 a          955 c         1078 g          958 t
ORIGIN      
        1 gaagcgcggc aggttcaacc gcacgctgcg ggcgttcggt ccctgatcga taatgatgtc
       61 cagcttgaaa tcttccatcg ccggatagag atattcctcg atggtttttt gcaggcggtg
      121 gatttcgtcg atgaacagaa catcgccttc ttcaagattg gtgagcaggc cggcgaggtc
      181 gcccgctttt tcaatggtcg ggccgctggt gcttttgaga tttgcgccca tggccttagc
      241 gatgatgttc gccagcgtgg tcttgccgag gccgggcgga ccgctgagca agatgtgatc
      301 aatcgcctcg ctgcgttgac gcgcggcggt gacggcgatt tcgagccgtt ccttgacctt
      361 gggctggccg gtgaattcgg aaaacaaaga gggccgcaac gtcatttcca acgcggcatc
      421 gggcttgatc aagacatccg aaatcattcg ttcagccatt gcaacaatac attgcttaac
      481 gcggcccgcc taacaaggca aaagacggag agactgagtt tggtttaaaa tgcttgaccc
      541 gaggcaagtt ggtgataaga aaatccgcga ctaatgtcag gagatcctca agcaggcaca
      601 cgtgccacct caaacaaatt tctttttcct ccctggctgt ggcctgtact ggtttccgga
      661 ctggcgtggg cggtgatttt tctggcgatc cctccggaca ggcaggattt tccgcttggc
      721 gatgactggg cattttcgca cagcgcaatc tggtttgcgc atgggctggg cattcattat
      781 gtgaaatggg cgagcatgcc gcaattggga caatggctct ggtcatggcc gtttttgcac
      841 atcatccatt ggccgcatgt cgccctgcgg ttgtcggcca tcgtgttgtc atggctggga
      901 ctggccgcgt tttgcgattt gctgcggctg gaaaatgttc cggagcgggt ggcggcattt
      961 gcggcatgtg tgctggcctt ggaccccctc ttttttatct cgcaggggga cttacatgac
     1021 ggatgtgccg gcgctgtcgt ttgggttgat cgcgctggct ttctattcgc gggctctggc
     1081 tgaaaaaaga cttcgttggc ttccagcggc aactctcttt gcggtgttcg cagtcatcac
     1141 gcggcaaacc atgctgatga ttccattggc cgccgctgtg ccgatgctgc ggaaccagga
     1201 aatgcgacgc agcgcggcgt ggggtctttc gattctcgtg ccgctggcgg tttgtgtcta
     1261 tgcgagccgg tggttttcgc agaggccgga cgtggagccg atgcatgcga agtttaatct
     1321 ggaaagcatg gcattccgtc cgtttgtggc tttgcatctg gcggggctgg tcgtgctgcc
     1381 gctgattttc ctggcaggga ggcagagaag ctggcgtgta ttttttgcgg cattcacggc
     1441 catgctgctg gcagccggtt tctatcattc gctggccgcg cgattgcctt atggcgggtg
     1501 gtttccttat tgcatgggga tgttgagtct tgagggaacg tacccggatg ggttggtcgt
     1561 ggggcagcgc gatattctgc tcacaccaac ggtgcaggtt gaactcacgg ttctcggttg
     1621 cgcaggcgcg gcgcttgttc tcgccgcgct atgggaaatg attcgtgccc ggaaatttcc
     1681 tggcgctctg ttggtgttca cctcgctgca gttttgcatc ctgctgatca tgccggtcat
     1741 ggcggatcgc tacttggagg tcttgtttcc cggcgcgatt tttttggccg ccacgtgttg
     1801 ttccatggct ggcatcggac ggcttgcggg cattgtggct gtggttgtga gcgggttcat
     1861 cgcggtggcg ttgtgtcacg attgctttgc atggaactcg gcgcgatggg aactcggacg
     1921 ggaggccatt gccgcaaagc gcatccatcc cgcggacatc gaaggcggct tcgaatggaa
     1981 cggctggtat gaacgaaacg atcccgcctg ggcgcctgtt tcaaatgttt cacccgtggc
     2041 ggacaaggat cccgcttcgc tgacgcttcg gttctcccgc atctattttc cgacggtgac
     2101 cgggcagtac gcgctggcgt tctctcagcc tagcaacacc attattattg cgacgatgcc
     2161 ttactcgacc tggcttccgc cggcaaagag ggattttttc ctcgtccagc aaaagccgca
     2221 gcgttgaatt caccgcagac tgggttgttc tcgggggcaa aacgagggtt gcattctcga
     2281 gcctttgcgc tatcttccgc tgcatcaccg atggtactgg gattctcaac aggactattc
     2341 aagcggtcat caaaaatgca tcaggctgca ttcgggatga aatgcgcatg gacgaagata
     2401 aaatcgcaca acaattattg caaaacggtc aattttaaag ggaaactgct cgaaacagag
     2461 gctcctttgg acagcgtaaa acattatcta atgcaccgac atttatgtgt gatagtattt
     2521 ctaatgaatc taacatctag ttttggagaa acttgcatcc gtaaaaactt cgaaattcaa
     2581 aaatttctcg cacagtgtcg gcagctttgt tataaatagt ctcacaataa ccctatgtat
     2641 aaaattaaat catggttctg gattcttctg gctcttttgg gcacttcgac gggcgcattt
     2701 gccagcgaaa cggacattaa cattcccgat cttagcgggg tcagttttcc cggcttgggc
     2761 ggcgtgagcg ggccaacctt gatgtatatc ggcattgccg tgtgcgtggt cggggcattg
     2821 tttggattgc tgcaatatac gcagaccaag gcgttgccgg tgcattcgag catggcgaac
     2881 gtttccaaca cgatttggga aacgtgcaaa acctacctgt tcacgcaggg caaattcctg
     2941 atgatcctct gggttttgat cgcggcgtgc atgatctatt actttggtca tctccaacaa
     3001 aataccgcca gtcatgtcgc ggtcattctg ctcgcgtcca ttttgggcat cctcggttct
     3061 tacggcgtgg cgtggttcgg catccgcatc aatacggttt ccaattcgcg cacggcattt
     3121 tccgcgctca agggcaatcc gctcgccacg ctcggcattc cattgcgttc gggaatgagc
     3181 gtcggcctgc tgctggtcgc ggtggaattg tttttcatga tttgcatcct gatgttcctg
     3241 ccccgcgaac tcgtcggtcc gtgtttcatc ggattcgcca tcggcgaatc gctcggcgcg
     3301 agcgtgcttc gcatttgcgg cggcattttc acgaagatcg ccgacatcgg ctcggacttg
     3361 atgaaaatcg ttttcaaact gccggaagat gaccccaaga acccgggcgt catcgccgat
     3421 tgcacgggtg acaacgctgg tgactcggtc gggccaacgg cggacggttt tgaaacttac
     3481 ggcgtgaccg gcgtggcgct gatcgcgttt ctcgcgctgg cgctccacgc agccgaatcg
     3541 caggcgatct gtgccaagct catcatctgg ctgttcacca tgcgcgccct gatgatcgtg
     3601 acgtcgctgg tttcctactt tgcgaacgaa atgctcagca agacgatgtt cggcggcaaa
     3661 aaagacttcg atttcgaagc gccactgaca cat
//
LOCUS       DASZMM010000120         5222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5253766, whole genome shotgun sequence.
ACCESSION   DASZMM010000120 DASZMM010000000
VERSION     DASZMM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5253766"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..2018)
                     /locus_tag="VG754_03005"
     CDS             complement(168..2018)
                     /locus_tag="VG754_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF18203.1"
                     /translation="MPEHPKKKGLSTGTKLRLFAAVVVLFVAAGFATLFYLNREPSYK
                     GTTLTEWVNDLVVINDHPRDTPAAIAIRQIGTNAIPYLIKWLNPHQQPSRFRKLMERL
                     SDASGGSIDLPETPNRTVEATEAFKILGPIAKPAIPVLEQLLHDTNFDVAVAYSLANI
                     GSDSLPVLTNALGSTNESVRRNALLGLGRLGYEAQTAAPLVESLIHDTNSITSRTAIL
                     NLGQLGKGVTPYIPELNEMLQTNHHAIEAAMALFHLGYTSELLRYLTNDSTKLRETCK
                     RVIVAFSPFRFDLKNVQHSTLATSGTVKGDSLNTRTDPRQHVDEETIQLLNAYLKSTN
                     ASERAQAADDLAFFGGCIFSSVAPLSKALSDTNEMVRHSAAEALKKIDLQIQDGGIIR
                     GPRKEKKIALEFTGHEFAEGGETILDELSGHRAKASFFLTGDFLRNPEFTPLVHRIIR
                     NGHYLGTHSDKHLLYCAWDTKKTIVTQNTFNADVPRNLAQFGFNYPSPFWIPPYEHYN
                     EEIAEWSSERGLILVNFTPGTRSNADYTQESDTNFVSSQAIFDSIMKKEREDPDGLNG
                     FLLLMHLGAGPGRKDKMHPRFGELLDALSAKGYQFVRIDELLGESGREKQ"
     gene            2496..3245
                     /locus_tag="VG754_03010"
     CDS             2496..3245
                     /locus_tag="VG754_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18204.1"
                     /translation="MLVLDELKKIGRSPGRLREVFRLRRWRQFFSILGFNSRAGNRWK
                     TAPGAPALKQREYSSYEQYVEHQQSKFKYLDLADYDVNYRRLLKERLQGLPIVKHGAN
                     ALCLGARQGTEVKAFLDLGCFAIGLDLNPGKENPFVLQGDFHHTQFPSQSVDIIFTNS
                     FDHAFDPQKLIAEIKRLLKPDGALIIEAIHGEAESDAPDNYASFWWQRVDDLVALLAS
                     NDFNLVRRIGFNNPWPGEQICFELQTSEAKS"
     gene            3277..3993
                     /locus_tag="VG754_03015"
     CDS             3277..3993
                     /locus_tag="VG754_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18205.1"
                     /translation="MNINLESNSLAERGGRAFTLVELLVVIGIIGILASLLLPVVARG
                     KKAVARTQCSNNLKQLALAINMYADDHGDQLPGPIWQGLYPVYNSTTTLFMPYYIAKY
                     LSLPEPSPAVLGVKVAVCPASAKITHQSLDGTLTTTLRQPISYIETITITNVQDDIVS
                     RPFGYPYEHLPNNNGYTNEPPKHLKEIRNPSSSWALQDTDQQNAVSLAQYYQFIPSSP
                     AHDSVRNNLFFDWHVEAVKP"
     gene            4099..4905
                     /locus_tag="VG754_03020"
     CDS             4099..4905
                     /locus_tag="VG754_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HWF18206.1"
                     /translation="MAEYKVKFEAFEGPLDLLLYLIKKEEVDIYDVNLTQLATQFIEY
                     IEVMRLLDLEIAGEFLVMASTLMYIKSRELLPLDQQVATEEEEDGEDPRWELIRQLVE
                     YKKFKDAAAQLQVLEERQEAIFPRQPVKPEFAEEPRGKPEATLFDLLNAVNSILKRVN
                     LRDDSRDVFEDKWTVSEKIEQIMKAIAEKARLKFSELFAGAMSRTEVVVTFLALLELI
                     RLKQLVAMQSEAFGEIEICRSETPAQVIVPTAESQSTGEQSVVGPHPESH"
BASE COUNT         1306 a         1352 c         1318 g         1246 t
ORIGIN      
        1 ccagagcctg cacccctata ttgaaaggag gacggaatcc acggatgaac acgcatacac
       61 acggatacac acggattaaa acggagaaga cgagacacga attggcacga attgacacaa
      121 attcgccagg aatgatttga atttgtgaaa aattcgtgta attcatgtca ttgcttttct
      181 ctgccgctct cgcccaacaa ttcatcaatc ctcacaaact gatagccttt ggccgaaagc
      241 gcatcgagca attcgccaaa acgcgggtgc atcttgtcct tgcggcccgg tcccgcgcca
      301 agatgcatca gcaacaagaa gccgttcaac ccgtccgggt cttcgcgctc ctttttcatg
      361 atgctgtcga aaatggcctg cgacgaaacg aaattcgtgt cgctttcctg cgtgtaatcc
      421 gcattcgaac gcgtgccggg cgtgaagttg accaggatta acccgcgttc gctgctccat
      481 tctgcaatct cctcgttgta gtgctcataa ggcggaatcc aaaatggact tggataatta
      541 aatccgaatt gtgccaaatt acgcgggaca tccgcattga atgtgttctg ggtcacaatc
      601 gtctttttcg tatcccaggc gcaatagagc aaatgcttgt ctgaatgagt gcccaggtaa
      661 tgaccgtttc ttataatacg atgaaccaac ggagtgaatt cgggattgcg cagaaagtct
      721 cctgtcagaa aaaatgacgc ctttgcgcga tgccccgaca gctcgtctaa aatcgtttcg
      781 ccgccctcgg caaactcatg cccggtaaac tccaatgcga ttttcttttc ctttcgtggg
      841 ccgcgaatga tgccgccatc ctgaatttgc agatcaattt tcttgagcgc ttccgccgca
      901 ctgtgacgta ccatttcatt ggtatctgat agagccttgg agagcggagc tactgatgaa
      961 aagatgcagc ctccgaaaaa cgccaaatca tcagcggctt gggcgcgttc tgaggcattt
     1021 gtggatttca aataagcatt caagagttgg atggtttctt catccacatg ttgtcggggg
     1081 tcggttcgag tgtttagaga atcgccttta acagttccag acgttgctag ggttgaatgc
     1141 tgaacgttct tgaggtcaaa gcgaaaagga gaaaatgcaa caattaccct tttgcaagtc
     1201 tcccgcagct tcgtggagtc gttcgtcaaa taccgaagaa gttcagaggt atatcccaag
     1261 tggaatagcg ccatggccgc ttcgattgca tgatggtttg tttgaagcat ttcgttgagt
     1321 tcaggaatgt atggcgtgac acccttccct aactgtccca agttcagaat ggctgtccga
     1381 gaagtgattg agttcgtatc atgtatcagg gactctacta aaggtgctgc agtttgcgct
     1441 tcatatccca atcgacccaa ccccaacaat gcatttctgc gcacactttc gttcgtcgat
     1501 cccagtgcat tggtgagcac tggcaatgaa tcggagccta tatttgccaa gctgtacgct
     1561 acagccacgt caaaatttgt atcgtgcaaa agttgttcca atacaggtat ggcaggtttt
     1621 gcaatcgggc cgagtatctt gaatgcctcc gttgcctcca cagtacgatt tggtgtttcc
     1681 ggcagatcga ttgagccacc agaagcatct gaaagtcgct ccatcaattt tcggaatctg
     1741 gaaggctgct ggtgtggatt taaccattta atcagataag gaatcgcatt ggtgccaatc
     1801 tgacgtatag cgatcgctgc gggcgtatcg cgcgggtgat cattaataac aaccaaatca
     1861 ttcacccact cggtcaacgt cgtgccttta taacttggtt ctcgatttaa gtagaacaat
     1921 gtcgcgaaac ccgccgcgac aaacagcacc acgacggctg caaaaagtcg cagctttgtt
     1981 ccagtggaca atcctttctt tttcggatgc tctggcatcg aattgataat tgcctcgaat
     2041 gacgttgaac ggcgaggaga atttccggct gaggcgtgaa atgaatttgg aatgtgtaaa
     2101 acattcaaaa atcgtaacac tgatgcatca tgtgaaatgt ttcatttgac caaatagaaa
     2161 aatatcatca ctctaccgca tcgccggatt tcgatgcggt agagtgcgtt tttaaatgca
     2221 ttgtttttac aagtttctca taaaactgga gcgtggctgt gtgcgaagcg ccagccgcag
     2281 caaaccatca aaatcctgag tcttccaagc tgctgcgact tgtcctgcgg acacagtcgc
     2341 gttccgctct ccatttatga gatgacttgt agttttgttg tttccgggat tataaacttt
     2401 tcagttatat ccaaggcgtt cacagcctca ctctttactt ccccctgcaa aaccgctcgt
     2461 gcatagttca tggagaaaaa ctgcaatcca caaagatgct cgtcctcgat gaactgaaaa
     2521 agattggccg gtcgcccgga cgattgcggg aagtttttcg gctgcgacga tggcggcagt
     2581 ttttcagcat cctcgggttc aactcgcgcg cgggcaatcg ctggaaaacc gcgcccggcg
     2641 cgcccgcgct caagcagcgc gaatattcct cctacgaaca atacgtcgag catcagcaat
     2701 cgaaatttaa atatctcgac ctcgcggatt acgatgtcaa ttaccgccgg ttattgaaag
     2761 agcgcttgca aggattgccc attgttaaac acggcgcgaa tgcgctttgt ctcggtgcgc
     2821 gccagggcac ggaagtcaag gcgttcctcg atctggggtg ctttgcaatc ggcttggact
     2881 tgaatccggg caaggaaaat ccgttcgttc tgcaaggcga ttttcatcat acgcaatttc
     2941 cgtcgcaatc ggtggacatc attttcacga actcgtttga tcatgcgttc gatccgcaaa
     3001 agttgatcgc ggaaatcaaa cgcctgctga agccggatgg cgcgctcatc atcgaagcca
     3061 tccacggcga agcggagtct gacgcgccgg acaattacgc gagcttttgg tggcaacggg
     3121 tggatgattt ggttgcgtta ctggcatcca acgatttcaa ccttgtgcgc cgcatcgggt
     3181 tcaacaatcc atggccgggc gaacagattt gttttgaatt gcaaacttcc gaagcaaagt
     3241 cataatctgc gccgggtcac aatggcaaag gagcaaatga atataaatct cgaatcgaac
     3301 tcgctggccg aacggggcgg cagggccttc acgttggttg aacttctggt ggtcatcggc
     3361 atcatcggga tactcgcgtc gttgttgttg ccggtggtcg cgcgcggaaa aaaagccgtt
     3421 gcgcggacgc aatgctcgaa caatctcaaa caactcgcgc tcgccatcaa catgtatgcg
     3481 gacgaccacg gcgaccagct tcccggcccg atctggcagg ggctttaccc ggtgtataac
     3541 agcaccacga cacttttcat gccgtattac atcgcgaaat atctcagcct gcccgaacca
     3601 tcgcccgctg ttttaggcgt gaaggtggcg gtttgccctg cctcagcaaa gatcacccat
     3661 caatcgctcg atggaacgct cacgaccaca ttgcgccagc ccatctccta tatcgaaacc
     3721 ataaccatta cgaacgtgca ggacgacatt gtttcgcgcc cgttcggtta tccttatgag
     3781 catctgccga acaacaacgg ctacacaaac gaaccgccca agcatctcaa ggaaatccgc
     3841 aatccttcct cgtcgtgggc cttgcaggac accgaccagc aaaatgccgt cagcctcgcg
     3901 caatattatc aattcattcc ctccagcccc gcgcatgaca gcgtccgcaa caatcttttc
     3961 ttcgactggc acgtggaggc ggtgaagcct taaccagata aaaaccgacc actacatttt
     4021 cccgtgttcg gaattgccaa gcggggacgg tcggccctaa gatggctttc cggttggaca
     4081 aaacgcgaac cgggcgcgat ggctgaatac aaagttaaat tcgaggcttt tgaagggccg
     4141 ctcgacttgc ttctttacct gatcaagaag gaggaggtgg acatctatga tgtcaatctc
     4201 acccagttgg ccacgcaatt catcgaatac atcgaggtga tgcgattgct ggacctggag
     4261 atcgccggcg aatttttggt gatggcttcc acgctgatgt acatcaagag ccgcgaattg
     4321 ctcccgctcg accagcaggt cgcgaccgag gaggaggaag acggcgaaga tccgcgttgg
     4381 gaattgatcc ggcagcttgt cgaatacaaa aaattcaagg acgccgccgc gcaattgcag
     4441 gttttggagg aacggcagga ggccattttc ccgcgccaac cggtcaaacc ggaattcgcc
     4501 gaggaaccgc ggggcaaacc ggaagcgacg ctgttcgacc tgctcaacgc ggtcaattcc
     4561 atcctcaaac gggtcaatct acgcgacgat tcgcgcgatg tgttcgagga caaatggacc
     4621 gtcagcgaaa aaatcgagca gataatgaag gccatcgccg aaaaagcccg gctgaaattt
     4681 tccgaactgt ttgccggcgc gatgagccgc accgaagtgg tggtgacgtt tctggcgttg
     4741 ctggaattga tccggcttaa acaactggtt gcgatgcagt cggaggcgtt cggggaaatt
     4801 gagatttgcc gcagcgaaac tccggcccaa gtgatcgtgc cgaccgccga atcgcaatct
     4861 accggcgaac aatcggtcgt tggtccgcat cccgaatcgc attgaaccca tgcacaactt
     4921 agccacgatg agtcttggtt taaaacggcg attgcaaccg cgatatgcaa acatgctaaa
     4981 caggtctgca acattctccc tctccccggg ggagagggtt ggggtgaggg tgagcgttag
     5041 gataaatttt ggccgttgtc attttcacag attcaataac tcatggaact gaaattcatt
     5101 ttggaatcat tgctgttctc ggcgcaaaaa ccgatgagcg cgcgcgaatt gcgcgacgtg
     5161 ctcaccgaag ccgctgcaag cgccgaaggc gacgaaaccg tccgcgcatt gaaaaaagtc
     5221 aa
//
LOCUS       DASZMM010000121         6081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3608562, whole genome shotgun sequence.
ACCESSION   DASZMM010000121 DASZMM010000000
VERSION     DASZMM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608562"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VG754_03025"
     CDS             <1..435
                     /locus_tag="VG754_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18207.1"
                     /translation="EVAHVIDDLQARAAEKSVALVNEMPADLQARADTDRLEQVLFNL
                     VENAIKYGRSEGKVTIGGLKNSDSKVELWVQDDGPGIPPDSRDRIFERFYRVDRARSR
                     ETGGTGLGLSIVKHIVQAHGGEVWVKSELGKGTTFFFTLPQA"
     gene            769..2250
                     /locus_tag="VG754_03030"
     CDS             769..2250
                     /locus_tag="VG754_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HWF18208.1"
                     /translation="MKPISAKLSRCLRATALLAAFGLAQVSHASWDTWGTDIRHNGVA
                     TLMTGEMMCPPSVNNDFLAGYLDVATNGYYDVKTYSQALRANGLRVVRTFWTPQTMSS
                     SPLPTSVYNAANVKNSALLTHGASTTYWEFFTNTINAITAGGIYVDLLCYNDGAMVGN
                     LSQFTASWQSIATTFKNNDNVFGYELVNEPQMASFGNSASTYYGDMQSIGNAVQAIDS
                     SKLIIVMPIQHWDDCSGIVGSLSGWYGKLVMSIHVYYGWGDMTSSWITSQHNTASNAG
                     TPVMVTETSFPNDGTEWSSTYPAFAQTVMSYKMPLTTYWPYYLAYSTLQTWNPTYGSF
                     MSQLYPQVLDGTYKIVNNNSGLVLDDVAWYKTNGSPVDTWAYQSQANEKWTVTYLSSV
                     GTTNTYKIVNVNSGLALEDPGWSTANGTRMDQWSYHGNNYYNEQWRISPVSQYSLNGW
                     FTVVNSYSGKTLEVYNSGTANGSIVDQWTATGGANQQWGFQAP"
     gene            2358..3260
                     /locus_tag="VG754_03035"
     CDS             2358..3260
                     /locus_tag="VG754_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18209.1"
                     /translation="MSRKIILAMAFALGVVMWAAHRPQPPVGDDSPPSVSAAAGVEST
                     QPPELRPIPTAQAETGASASPAPVEEGIEQQEAGIRLALQSTNMADRVHALGELLPAL
                     VRRDALEAARFADTLDPAIRDEALSRVARAWAARDPEGALSWVSQFPISEQRTRMLND
                     CFFEIALADPAQAVAAAQRLGLGEDQPAVLANLAQQWAANDLSGSLSWAENQPSGALR
                     DQLLARIAFVQSQSNPADAARLVVEQIPPGPEQVEAAISVVHQWGQADMASARSWVRS
                     FPAGPIQDRAYAELEGIAQGTTQQ"
     gene            complement(3336..4796)
                     /locus_tag="VG754_03040"
     CDS             complement(3336..4796)
                     /locus_tag="VG754_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF18210.1"
                     /translation="MNYKTEIDCEARKLLTRRHFFRDCGVGLGAIALASLLNETKVFG
                     ATSATVIKNGDPLAPRAPHFPGKAKRVIYLFMAGAPSQLDMFDYKPTLKKYDGQPVPA
                     EVVKDQRYAFIRRDAALFATTNKFQKYGQCGAELSEVLPHLSEVVDDIAIVKSMTTDA
                     FNHAPAQIFMNTGATQFGRPSMGSWVTYGLGSESQSLPGFVVLSSASGTSGGASNWGC
                     GFLPTVFQGVPFRRSGDPILSLSNPAGITPQMQRQSLDLLKGLNQHHLDEVGDPEIAT
                     RINAFEMAYRMQTSAPELMDISKESKETLDMYGAETGKSSFANNCLLARRLVERGVRF
                     VQLYHEAWDHHSEVIKGVKDQCGLTDKPAAALIKDLKQRGLLEDTLVVWGGEFGRTPM
                     VETNESAGRKMGRDHHPQAFTMWMAGGGIKPGLTLGETDDFGFHIARDRVHVHDLHAT
                     LLYLLGFDHTKLTYHFQGRDFRLTDVEGELVPKLFA"
     gene            complement(4944..>6081)
                     /locus_tag="VG754_03045"
     CDS             complement(4944..>6081)
                     /locus_tag="VG754_03045"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HWF18211.1"
                     /translation="CAEANKQIAALQSNWPAADTTMILAARPEPRVTHVFQRGDFRNP
                     AEVVQPGVPAFMPPLPKDAPLNRLTLARWLVDEKNPLTARVMMNRIWQAYFGRGIVVT
                     AEDFGTQGDKPSHPELLDWLATEFMRRHWDVKAMQRLIVESATYRQSSKITPEMYVTD
                     QYDRMLERGPRFRVEGEVVRDIALRASGLLSEKIGGPSIYPPIPDGVLKLGYGAPMKW
                     ETSTGEERYRRGLYIFWKRTVPYPSLSIFDAPNADVTCVRRVMSDTPLQALTTLNDTV
                     FTEAAQAMALRVWKEGGHDDRERAIYAFRLCTGRKPDAVELERVLALVKQQENYFEDR
                     TTSAITVASNDPKNPTPNVNLHAVAAWTVASRVLLNLDETITKE"
BASE COUNT         1293 a         1817 c         1679 g         1292 t
ORIGIN      
        1 gaagtggcgc acgtcattga tgacttgcag gcgcgcgccg ctgaaaaatc ggtcgcgctc
       61 gtcaacgaaa tgcccgccga tttgcaggcg cgcgccgata ccgaccgctt ggaacaggtg
      121 cttttcaacc tcgtcgagaa cgccatcaaa tacggacgct ccgaaggaaa ggtcacgatt
      181 ggcgggctga agaattccga cagcaaagtc gagctttggg tgcaggacga cggcccgggc
      241 attccgcctg attcgcgcga ccgcattttc gaacggtttt atcgcgtgga ccgcgcccgc
      301 tcacgcgaaa ccggcggcac cggcctgggc ctttccatcg tcaaacacat tgtccaagcc
      361 catggcggtg aagtttgggt caagagcgaa ctcggcaagg gaaccacttt ctttttcacg
      421 ctgccacaag cgtgaacata gtcccgctcc gttctccatt tatgagatga cttgcagatg
      481 cttcctttat ctcgaacctg ctgaacatcg gcgccgcaat cgttccggcg aacacctggc
      541 cgcttcgtaa atacaaattg gtaatcgatt tgataaatca ggcaatggat tttcaaataa
      601 gcaaggataa gatatgcgct gatcacaggc gtatgagcga agctcttcat cggctttgcg
      661 ctgcatctac aatgcgcggc agatgagcct ttgagccggc ggaaatcgca catccggcaa
      721 ttaagaacag caaccaaaaa ccctgacaag agaaaggact catccataat gaaacccatc
      781 agtgcaaaac tcagccgatg ccttcgcgcc acggctctac tggccgcatt tggccttgcg
      841 caggtctccc acgcttcctg ggacacttgg ggaacggaca tccggcataa cggcgtcgcc
      901 accttgatga cgggagaaat gatgtgcccg ccttcggtaa acaacgattt tctcgctggc
      961 tacctggatg tcgcgaccaa cggttattat gatgtcaaaa cctacagcca ggcgctgagg
     1021 gcaaatgggc tgagggttgt gcgcactttt tggactcccc aaaccatgtc gtcaagcccc
     1081 ctgccaacca gcgtgtacaa cgccgccaac gtcaaaaatt cggccttgtt gacccacggc
     1141 gccagcacca cctattggga atttttcaca aacaccatca atgccatcac cgccggcgga
     1201 atctatgtcg atcttctctg ctacaacgat ggcgcgatgg tgggcaacct gtcccagttc
     1261 accgccagtt ggcaaagcat tgcgacaact ttcaagaaca atgacaatgt ctttggctac
     1321 gaattggtga atgagccgca gatggcgagc tttggcaaca gcgcctccac ttattatggc
     1381 gacatgcagt ccatcggcaa cgcggtccag gcgatagaca gcagcaagct tatcatagtc
     1441 atgccgattc aacattggga tgattgctcc gggatagtcg gaagcctttc cggttggtat
     1501 ggcaaattgg tcatgtcgat ccatgtctat tatggctggg gcgacatgac ctcctcgtgg
     1561 atcaccagcc agcacaacac cgcctccaac gccggcacgc cggtaatggt caccgaaacg
     1621 tctttcccca acgacgggac cgaatggagc agcacttacc cggccttcgc ccagaccgtc
     1681 atgtcctata aaatgccgct gacgacgtat tggccctatt acctggcgta tagcacgctc
     1741 cagacatgga acccgaccta tggctcgttc atgtcgcagc tttatccgca ggtgctcgac
     1801 ggcacttaca aaatcgtcaa caataacagc ggcctggtcc tcgatgacgt cgcctggtat
     1861 aagaccaatg gctcccctgt ggacacttgg gcctatcaaa gccaggcaaa cgaaaagtgg
     1921 acagtcacct acctttcctc cgtcggcacg acgaatacgt ataagatcgt caatgtgaac
     1981 agcggcttgg cgttggaaga ccccggctgg tcaaccgcca atggcacgcg catggaccag
     2041 tggtcgtatc atggcaataa ttattataac gaacaatggc gcatctcccc ggtttcccag
     2101 tactccttaa acgggtggtt tacggtcgtg aacagctaca gcggaaagac cttggaggtg
     2161 tacaactcgg gcaccgcaaa tgggtccatc gtggatcaat ggaccgcgac tggaggcgcc
     2221 aatcagcagt ggggtttcca ggcgccgtag ttcaaatcct gccggttgag aacgaagttt
     2281 gtcacaggca ggcggatgca tggggtatgc ggctgcctgc ctggacactt caactggtgt
     2341 ttgcagcctg attgaatatg agccggaaga ttattttggc aatggcgttt gcgcttgggg
     2401 ttgttatgtg ggcggcgcat cggccacagc ccccggtcgg ggacgattct ccgccttctg
     2461 tttcagccgc ggcaggcgtt gagtcaaccc agccgcccga actgcgtccg attcccactg
     2521 cgcaggccga aaccggcgct tccgcttcgc ctgcgccggt tgaagaaggc atcgaacaac
     2581 aggaggccgg cataagactg gcgttacagt caacgaatat ggcggaccgc gttcatgcct
     2641 tgggcgagct gctgcctgcg ttggtgcgcc gcgatgctct cgaggcggcg cggtttgccg
     2701 acacgcttga cccggccatc cgcgacgaag cattgtcccg cgtggccagg gcgtgggcgg
     2761 cccgggaccc tgaaggcgcg ttaagctggg tttcgcagtt tcccatatcc gagcagcgga
     2821 cccggatgtt gaacgattgc ttttttgaga ttgcccttgc cgatccggcc caagcggtgg
     2881 cagcggcaca acgccttggt ctcggggagg accagcccgc ggtgctggcg aatttggcac
     2941 agcaatgggc ggcgaacgat ctctccggca gtttgtcgtg ggctgaaaac cagccttcgg
     3001 gggccttgcg cgaccagttg cttgcccgca ttgcctttgt ccagtcccag tcaaaccccg
     3061 ctgatgccgc ccgtttggtg gtcgagcaaa tcccgcccgg gcccgagcag gttgaggccg
     3121 ccatttcagt ggttcaccaa tgggggcaag ccgatatggc aagcgcgagg tcgtgggtcc
     3181 ggagctttcc cgcaggcccg atccaggacc gcgcctacgc ggaattggag ggtatcgcgc
     3241 aaggaaccac tcagcagtga acacaccgga gactcaaagc ctgtagaggg tcacttactg
     3301 gcccctgact gttgacacaa atttgatttt ccttgtcacg caaaaagctt cggcaccaat
     3361 tcgccttcca catccgtcag ccgaaaatcg cggccttgga aatgatacgt caacttcgtg
     3421 tggtcgaatc ccaacaaata caagagcgtc gcgtgcagat cgtggacatg gacgcggtcg
     3481 cgggcgatgt gaaaaccaaa gtcatccgtt tcgcccagcg tcaatcctgg cttgatgccg
     3541 ccgccggcca tccacatcgt gaacgcctgc ggatgatggt cgcggcccat tttgcggccg
     3601 gcggattcgt tggtttcaac catcggtgtg cggccgaatt caccgcccca aaccaccaga
     3661 gtgtcctcca gcaatccgcg ttgtttcaag tctttgatga gggcggcggc gggtttgtcg
     3721 gtcaagccgc attggtcttt cacgcccttg atgacttcgc tgtgatggtc ccacgcctcg
     3781 tgataaagtt gcacgaaacg cacgccgcgc tcgaccaatc gccgcgccaa caggcaattg
     3841 ttcgcgaatg aactcttgcc ggtttccgcg ccatacatgt cgagcgtttc cttcgattcc
     3901 ttggaaatgt ccatcaattc cggcgcgctg gtttgcatcc ggtaagccat ctcgaacgcg
     3961 ttgatgcgcg tcgcgatttc cgggtcgccg acttcgtcga ggtgatgttg attgaggccc
     4021 ttgagcaggt cgagcgattg gcgctgcatc tgcggcgtga tgcccgcggg attggacagg
     4081 ctcaagatgg gatcgcccga acgccggaac ggcacgcctt gaaaaaccgt cggcaaaaat
     4141 ccgcagcccc aattcgacgc cccgccgctc gtgccgctcg ccgaactcaa caccacaaat
     4201 cccggcaacg actgcgattc gctgcccaag ccgtacgtca cccacgaacc catgctgggc
     4261 cggccaaatt gcgtcgcgcc ggtgttcatg aaaatttgcg cgggcgcatg attgaatgcg
     4321 tcggtcgtca ttgatttgac gatggcgatg tcatcgacga cctcggagag atgcggcaaa
     4381 acttccgaaa gctccgcgcc acactggccg tatttttgga atttattcgt cgtggcgaac
     4441 aacgcggcgt cgcgccggat gaatgcgtaa cgctggtcct tgaccacctc ggcgggcaca
     4501 ggctggccgt cgtatttttt gagcgtcggc ttgtaatcga acatgtccaa ctggctcggc
     4561 gcgcccgcca tgaaaaggta aatgacgcgc ttggccttgc ccggaaaatg cggcgcgcgc
     4621 ggcgcaagcg ggtcgccatt cttgattacc gtcgcagaag tcgcgccgaa aaccttcgtc
     4681 tcattcaaca acgacgccag cgcgatcgcg ccgagcccga cgccgcagtc acgaaaaaaa
     4741 tgacggcggg tcagaagttt ccgggcttcg caatcaatct cggttttata gttcatattt
     4801 ttaaattttt gacagaattc acagaatgaa ctgaatctaa aaccggtgaa ggttgtcctt
     4861 ccattcccgc atccattctg aaaattctgt tcattctgtc aaaatcgctt ttcgttgggt
     4921 gctaagtcat tgggtattta agttcactct ttggtaattg tttcgtcgag attcagcaac
     4981 acccttgacg cgactgtcca cgccgcgacc gcgtgcaggt tcacattcgg cgtcgggttt
     5041 ttcggatcgt tggaagccac tgtgatggcc gaggtggtgc ggtcttcaaa ataattttcc
     5101 tgctgcttca ccaacgcaag aacacgctcc aattccacgg cgtcaggttt gcggccggtg
     5161 caaagacgaa acgcataaat cgcgcgttcg cggtcatcat ggccgccttc cttccaaacg
     5221 cgcaaggcca tcgcctgcgc cgcttccgtg aacaccgtgt cattcaacgt cgtcagcgcc
     5281 tgcaacggcg tgtccgacat cacgcgccgc acgcaggtca cgtccgcgtt gggcgcgtca
     5341 aaaatcgaga ggctcggata tggcaccgtg cgtttccaaa aaatataaag cccgcgccgg
     5401 taacgctcct cgccggtgct cgtctcccat ttcatcggcg cgccgtagcc gagtttcagc
     5461 acgccatcgg gaatcggcgg ataaatgctc ggcccgccaa ttttttcgct cagcaatccg
     5521 ctcgcgcgca acgcgatgtc ccgcaccact tcgccttcca cgcgaaaacg cggcccgcgt
     5581 tcaagcatcc ggtcgtattg gtccgtcaca tacatttccg gcgtgatctt cgacgattgc
     5641 cggtaagtcg ccgattcgac gatcaaccgt tgcatcgcct tcacgtccca atgccgccgc
     5701 atgaattccg tcgcgagcca atcgagcaat tccggatgcg acggcttgtc tccttgcgtg
     5761 ccgaaatcct ccgccgtgac gacgatgccg cgcccgaaat acgcctgcca gatgcggttc
     5821 atcatcacgc gcgcggtcag cggatttttt tcatccacca gccagcgcgc aagcgtgagc
     5881 cggttcaacg gcgcgtcctt gggcaacggc ggcatgaagg ccggaacccc cggctgcacg
     5941 acttccgcgg gattgcgaaa atcgccgcgc tggaaaacat gcgtcacacg cggttccggg
     6001 cgcgccgcca aaatcatcgt cgtgtccgcc gcgggccaat tgctttgcaa cgcggcgatc
     6061 tgtttgttcg cctcggcgca t
//
LOCUS       DASZMM010000122         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2520007, whole genome shotgun sequence.
ACCESSION   DASZMM010000122 DASZMM010000000
VERSION     DASZMM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520007"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VG754_03050"
     CDS             <1..870
                     /locus_tag="VG754_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWF18212.1"
                     /translation="IGRSRKELDLLDASAVREFYRKEKPEYVFVAAAKVGGIFANDQQ
                     PVPFLFENLLIQNNLIHGAYEHGARKLLFLGSTCIYPKLAPQPLKEEYLLSGPLEPTN
                     QWYAIAKIAGIKLCEALRRQHGCDFISALPTNLYGPNDNYDLQTSHVLPALIRKFHEA
                     KIAGADTVTCWGTGAPLREFLHADDLAAACIFLMENYSDPNIINVGFGSDISIRDLAE
                     LVKNIVGFKGALVWDKSKPDGTPRKLTDSSRIFALGWKPKIDLATGIRMVYEDFAASH
                     QSRTAAVPAAAAV"
     gene            complement(939..2249)
                     /locus_tag="VG754_03055"
     CDS             complement(939..2249)
                     /locus_tag="VG754_03055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWF18213.1"
                     /translation="MGTKSTAALSGLLACLALTAFGQIEEMKPDDPFFAPYHPLKAPP
                     PGKHFLRAGDRLAICGDSITEQKMYSRIMETYLTVCTPELGISVRQYGWSGETAPGFL
                     ARMTNDCLRFKPTIATTCYGMNDHGYRPYQDSIGAAYQSNSMAIDESFKKHAVRLVEG
                     SAGCVGKKPFWVGDPNATVEQLNMNLCQLRNIGLLLAKQEKVAFADVFWPMLVEGHEA
                     QLKYGTNFAIAGKDGVHPNWAGHVVMAYAFLHQFGLDGDIGTFTVDMKSNKAKVSPGH
                     ELLGFKNGELQVRSHRYPFCIGNGDPSKDDNIHAGTMLVPFNSELNRLMLVVKHPQAN
                     SYKVTWGDETKIFKAEDLTRGINLAEVFLQNPFSDPFKKVDEAVAAKQAFETDEIKKR
                     FHGPEGKANMEGTAARAEQQRSVLVAAVKTAFVPVMHTIKIEPE"
     gene            2408..3673
                     /locus_tag="VG754_03060"
     CDS             2408..3673
                     /locus_tag="VG754_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWF18214.1"
                     /translation="MRAYNLKLFYCFDMLIWLLALILFGCLGYAGYAMGAIRAAASLV
                     GIIIAAILAPHLGRIFTPLLSALTVKNPMLLWVLGPFIAFLVVLAVFKIIGHAAHRKV
                     DVYYKYKAGDLRMGLFNRLNARTGIAVGVLNAAVYLVLISWVIYVFSYTTSQIAATDS
                     VAWYVKLLNQAGEGLQSSGMNKVTAAVDGMPDSYYQTADIAGLVYHNDLLEGRLTRYP
                     AFLAIGERPVFQDIGNDKEFSELRQRQPPISDIIDNPKAQAILNDPNQLKDIWAVLKP
                     TLPDLTTYLKTGQSAKYDAEKILGRWDFSLKGALNAFKRNNPKVTVPEMQRVKQALTM
                     CFAKTTFVATPEPDKLAFLKDYGKLIPPTKPKTPPTVDVKSFSGTWSGDGSKYDISLP
                     DKGGSFEGTVEGDTLIVTGDLLPMVFERQ"
     gene            complement(3889..>4330)
                     /locus_tag="VG754_03065"
     CDS             complement(3889..>4330)
                     /locus_tag="VG754_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18215.1"
                     /translation="LKLKRHEQPPPGYFNSFPTHVINRLERNAGNGGVLGDASWLMRF
                     MGLLETNPIAAGLFGMSLCGLLISGIAYSQSRPAYNYSGNSPATIDVADIGNETLQTP
                     QVTRTASVDSFAPTANSVFTTNAAALFDGAQSLSVTPVNWQVNR"
BASE COUNT          940 a         1204 c         1194 g          992 t
ORIGIN      
        1 attggccgct cacgcaaaga actcgatctt ctcgatgctt cggccgtccg tgaattctac
       61 cgcaaggaaa agcccgaata cgtcttcgtc gccgcggcaa aagtcggcgg catttttgcc
      121 aacgaccagc agcctgttcc gtttttgttc gagaacttgc tcattcaaaa taatcttatc
      181 cacggcgcat atgagcacgg cgcgcgcaag ctcctttttc tcggcagcac ctgcatctat
      241 cccaagctcg cgccacaacc gctcaaggaa gaatacctgc tctccggtcc gcttgagccg
      301 accaatcaat ggtatgccat cgccaagatc gccggcatca aactttgcga ggccttgcga
      361 cggcagcatg gttgcgattt catttccgcg ctgccgacca atctttacgg acccaacgac
      421 aactacgatt tgcaaacctc gcacgtgctt ccagcgttga tccgcaagtt tcacgaggcg
      481 aagattgcgg gcgccgatac cgtcacctgc tggggcacgg gcgctccgtt gcgcgaattc
      541 cttcacgccg acgaccttgc cgccgcctgt attttcctca tggaaaacta cagcgacccg
      601 aacatcatca acgtcggttt tggttcggac atttccatcc gcgaccttgc tgaactggta
      661 aagaacatcg tcggcttcaa gggcgcattg gtttgggaca aatccaaacc cgacggcacg
      721 ccgcgcaaac tcaccgacag ctcgcgaatt ttcgcgctcg gctggaaacc gaaaattgac
      781 ctcgccaccg gcatccgcat ggtctatgag gattttgcgg cgagccatca atcccggacc
      841 gcggctgtcc cagccgcagc agcggtttga tgaaagcagg agtggactca ttcggaacct
      901 gccgtgttta ccatctctct gcggctgccg cggtccaatc attccggctc aatcttgatg
      961 gtgtgcatca ccggcacaaa agccgttttt acagcagcga ccaggaccga ccgttgctgc
     1021 tccgcccgcg ccgcagtccc ttccatgttg gctttgcctt cgggaccgtg aaatcgtttt
     1081 ttgatctcgt ctgtctcaaa agcctgcttg gctgcgacag cttcgtccac ttttttgaac
     1141 gggtctgaaa atgggttctg caaaaaaact tccgccaggt tgattccgcg cgtcaaatct
     1201 tccgctttga aaatcttcgt ctcgtcgccc caggtcacct tgtaagaatt cgcctgcgga
     1261 tgtttcacga ccagcatcag ccggttcaac tccgagttga acggcaccag catcgttccg
     1321 gcatggatgt tatcatcttt cgacgggtcg ccgttgccga tgcaaaacgg atagcgatga
     1381 ctccgcacct gcaattcacc atttttgaaa ccgagcagct catgtcccgg cgacaccttc
     1441 gccttgttcg atttcatatc gaccgtgaac gtgccgatgt cgccatcgag tccgaactga
     1501 tgcaaaaatg cgtaggccat caccacatgc cctgcccaat tgggatgcac gccgtctttc
     1561 ccggcgatgg cgaagttcgt accatatttg agttgcgctt catgtccttc aaccagcatc
     1621 ggccagaaca catcggcgaa ggccaccttt tcctgtttgg ccaggagcag cccgatgttc
     1681 cgaagctggc aaagattcat gttcaactgc tccacggtcg cgttcgggtc gccgacccaa
     1741 aacggttttt tgcccacgca accggccgaa ccttcgacca accgcacggc atgtttcttg
     1801 aacgattcgt caatcgccat ggaattggac tgatacgccg cgccaatgct gtcttgataa
     1861 ggccgataac cgtggtcgtt catcccgtag caggtcgtgg cgatggtcgg tttgaaacgg
     1921 agacaatcat tggtcatgcg ggccagaaat ccgggggccg tttcgccgct ccagccgtat
     1981 tgccggacag aaatccccaa ctccggcgtg cacaccgtca ggtaagtttc catgatgcgc
     2041 gaatacatct tttgttcggt gatggaatca ccgcaaatgg ccagccggtc tccggcgcgc
     2101 aaaaagtgtt tgccgggcgg tggcgccttg agcggatgat acggcgcgaa gaaaggatcg
     2161 tccggtttca tctcctcgat ttggccgaag gcggtaagcg ccaggcacgc aagcaaaccc
     2221 gataatgctg ccgtagattt cgttcccata ttgttgtggg gcgaatgttt cccttttccg
     2281 ttatgcaaat caacagtgaa agacggcgct ggagtgatgg agttgtggag tattggacga
     2341 gggcctcaca tttcacggga gcttggatgt cggtgcttgg agcttacggc atggagtgtg
     2401 aaaaaatgtg cgtgcgtaca atttaaaatt gttctattgt ttcgacatgc tcatttggtt
     2461 gcttgctctg attttgttcg gctgcctcgg ttatgccggt tatgccatgg gcgcgattcg
     2521 cgccgccgct tcattggttg gaatcatcat cgcggcaatt ctcgcgccgc atctgggacg
     2581 tattttcacg ccgctattga gcgcattgac ggtgaagaac ccgatgctcc tttgggtgct
     2641 ggggccgttc atcgcgtttc tggtcgtgct ggcggtgttc aagatcatcg gccacgcggc
     2701 gcatcgcaag gtggatgtct attacaaata caaagcgggc gatttgcgca tgggcttgtt
     2761 caaccgcctg aacgcgcgga ccggcattgc cgtcggggtg ttgaacgccg cggtttacct
     2821 ggtgctcatc tcctgggtga tttacgtgtt cagttacaca acgtcgcaga tcgccgccac
     2881 tgacagcgtg gcttggtatg tcaaattgct gaatcaagcc ggcgaaggat tgcaaagctc
     2941 cgggatgaac aaagtcactg cggcggttga cggaatgccg gacagttatt accaaacggc
     3001 ggatatcgcg ggactggttt atcacaatga ccttttggaa gggcggctga cgcgttaccc
     3061 ggcgtttctt gcgatcggcg agcggccggt ttttcaagac atcggcaacg acaaggagtt
     3121 cagtgaattg cgccaaagac aaccgcccat cagtgacatc attgataatc ccaaggcgca
     3181 agccatcctc aatgatccga accagctcaa agatatctgg gccgtgctca agccgaccct
     3241 cccggacctg acaacctatt tgaagaccgg ccaatcagcg aagtacgacg cggaaaagat
     3301 tttgggccga tgggacttca gcctgaaagg cgcgttgaat gccttcaagc gcaacaatcc
     3361 gaaagtgact gttcccgaaa tgcaacgcgt caagcaggcg ttgacgatgt gttttgcaaa
     3421 gacgactttc gtggccactc ccgaaccgga taagctggcg ttcctgaagg attacggcaa
     3481 attgattcct ccgacgaagc ccaaaacgcc gccgacagtt gatgtgaaat ccttcagcgg
     3541 gacatggagc ggcgacggct cgaagtatga catttcgctt cctgacaagg gcggctcatt
     3601 cgagggaacc gttgaaggcg acacgttgat cgtcaccggg gacttgcttc ccatggtgtt
     3661 cgagcggcaa tagcctgcgg caccttcacg gtaaattgcg cctcgaccgg tttgagcaca
     3721 ttcgtgccgg ttgccaccga ggttaatttt agcgagggga gcttcggact tggaatggac
     3781 ggcaagccac aaaagggtga ttgcaaaaac gagaattaaa accgcaatgg cggcgaattg
     3841 aatggttcgc ttgcgtatcg ggttccccat tatacccggg tcgcgcgatc agcggttcac
     3901 ttgccagttg actggcgtca cgctcaacga ttgagcgccg tcaaaaagcg cggctgcgtt
     3961 cgtagtgaac acagagttgg cagtgggagc aaaggaatcg acactggcag tccgggtcac
     4021 ctgcggagtt tgcagagttt cattgccgat gtcggcgaca tcaatcgttg caggggaatt
     4081 gcccgagtaa ttgtaagcgg ggcgcgattg cgaataggcg attccggaaa tgagcaggcc
     4141 gcagagggac atcccgaaaa gtcccgcggc aatcgggttg gtttcgagca agcccatgaa
     4201 gcgcatgagc caggaagcat cgcccaacac gccgccgttc ccggcgtttc tttccaggcg
     4261 gttgatgacg tgggtgggga agctgttgaa ataaccgggc ggcggttgtt catggcgctt
     4321 cagcttcaac
//
LOCUS       DASZMM010000123         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4985488, whole genome shotgun sequence.
ACCESSION   DASZMM010000123 DASZMM010000000
VERSION     DASZMM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4985488"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VG754_03070"
     CDS             <1..687
                     /locus_tag="VG754_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF18216.1"
                     /translation="LVGFVFVDITSDDIQGYVDAASKRISETVKFPSGYYIQWAGQFE
                     YLKSAEQRLKIVVPFTLLIIFMLIYLNTKSVVKTAIVLLAVPFSLVGAFWFLHLLGYN
                     MSVAVWVGLIALAGLDAETGVVMLLYLDQAWEKFRAEGRMKSMDDLQAAVIEGAVHRV
                     RPKVMTICAILFGLLPIMWSPTTQSGADVMKRIAAPMIGGVITSGILELLLYPVIYVL
                     WRKRHVGIGD"
     gene            745..1515
                     /gene="rfbF"
                     /locus_tag="VG754_03075"
     CDS             745..1515
                     /gene="rfbF"
                     /locus_tag="VG754_03075"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HWF18217.1"
                     /translation="MKVVILCGGLGTRLREETEFRPKPMVPVGERPILWHIMKIYAHY
                     GYKEFVLCLGYKGDIIKDYFRNYQWNTSDVTLRLGARPQIKYHNVHDEEDWSVTLVDT
                     GQLTQTGGRLKRVLPYVDDTFLLTYGDGLINSNIAASVDFHHKTGKAVTLTAVQPASR
                     FGDLSINGDIVTEFREKTERQANFINGGFFVMNKRIGEYLTDDQCNLEQGPLNQLASK
                     GQIAAFQHTGFWQCMDTYREQQLLTELWLKGKAPWKIW"
     gene            1517..2617
                     /gene="rfbG"
                     /locus_tag="VG754_03080"
     CDS             1517..2617
                     /gene="rfbG"
                     /locus_tag="VG754_03080"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HWF18218.1"
                     /translation="MFNGFYQGRRVLVTGHTGFKGAWLSLWLKELGATVWGVSLPAPT
                     NPNFHEVIAEHTFKGEIECDIRDSESLSKAVKKIKPDVVFHLAAQPLVRLSYAQPIET
                     LQTNALGTANLLEAVRKTELPCPVVVVTSDKCYENREWEFAYRENDPLGGHDIYSMSK
                     AATELVAQAWNRSFFKPDKKLGPVVTARAGNVIGGGDYAADRILPDCVRSLIAKKPIL
                     VRNPHAVRPWQHVLECLSGYLWLAARLEGEDKMSRLAGAFNFGPEPSSRQPVQKLVEE
                     VLTHWPGKWVDGSDPNSPHEATLLTLSIEKAGALLQWSPVWDFQEAVGRSIEWYHERH
                     ALKNKEMLALSNAQIHAYVEAAKRKKIAWAVS"
BASE COUNT          575 a          669 c          819 g          612 t
ORIGIN      
        1 ctcgtcgggt ttgttttcgt ggacatcacg agcgacgaca tccaaggcta cgttgatgcc
       61 gcgtcaaagc gcatcagcga aaccgtgaaa tttccgtctg gttattacat ccaatgggcg
      121 ggccagtttg aatatctcaa aagcgcggag cagcggctca agattgtcgt gccgttcact
      181 ttgctgatca ttttcatgct catttacctg aacacgaagt cggttgtgaa aacggccatc
      241 gtgctgcttg cggtgccgtt ctcgctggtc ggcgcgttct ggtttctcca tctgctcgga
      301 tacaatatga gcgtcgcagt atgggtcggt ttgattgctc tagcgggtct cgatgccgaa
      361 actggcgtcg tgatgctgct ctaccttgac caggcgtggg agaagttccg ggcggaaggc
      421 cggatgaaat cgatggacga tttgcaggcg gcggtcatcg aaggcgcggt ccaccgcgtc
      481 cgaccaaaag tgatgacgat ttgcgccatt cttttcggtt tgctgcccat catgtggtcg
      541 cccaccacgc agagcggagc ggacgtgatg aagcgcatcg ccgcaccgat gatcggcggg
      601 gtcatcactt ccgggatttt ggaattgctt ttgtatcccg ttatttacgt tctttggcgc
      661 aagcggcacg tcggaattgg ggattgattg tcgggaagcg gtcatttaac gtaaccattc
      721 gccggtcgca tggccggttt cattatgaaa gttgtcattt tatgcggcgg tttgggaacg
      781 cgcttgcgcg aggaaacgga attccggccc aagccgatgg tgcctgtcgg cgagcgcccg
      841 atcctctggc acatcatgaa aatttacgcc cattatggct acaaggagtt cgtcctttgc
      901 ctcggttaca agggagacat catcaaggat tatttccgca attaccaatg gaacaccagc
      961 gacgtgacgt tgcgcctcgg cgcgcgtccc caaatcaaat atcacaacgt ccacgacgag
     1021 gaagattggt cggtgacgct ggtcgatacc gggcaattga cgcagacggg cggacggttg
     1081 aagcgggttt tgccttacgt tgacgacacg tttttactga cttacggcga cggcttgatc
     1141 aacagcaaca ttgccgcatc ggttgatttt catcacaaga cgggcaaggc ggtcacgctc
     1201 acggcggtgc agccagcaag ccggttcggc gacttgagca tcaacggcga catcgtcacg
     1261 gaatttcgcg agaagacgga gcgccaggcg aatttcatca acggcggttt ttttgtgatg
     1321 aacaagcgca tcggcgaata tctcaccgat gaccagtgca atcttgaaca aggcccgctg
     1381 aaccagcttg cgtcgaaggg ccagatcgcg gcgttccagc acaccggttt ctggcagtgc
     1441 atggacacgt atcgcgagca gcaattgctg acggaattgt ggctcaaggg caaggccccg
     1501 tggaagattt ggtgaaatgt tcaacggctt ttaccagggc aggcgcgttc tcgtcaccgg
     1561 ccacaccggt ttcaagggcg catggctttc gctttggctg aaggaactcg gcgcgacggt
     1621 ttggggcgtg agtttgcccg cgccgacgaa tcccaatttc cacgaggtca ttgccgagca
     1681 cactttcaaa ggggaaatcg aatgcgacat ccgcgattcg gaatcgcttt ccaaggcggt
     1741 gaaaaaaatc aagccggacg tcgtgtttca tctcgccgcg caaccgttgg tgcggctttc
     1801 ctacgcgcaa ccgattgaga cgcttcaaac gaacgcattg ggaacggcca atctgctcga
     1861 agcggtgcgc aagacggaat tgccctgtcc ggtggtcgtg gtgacgagcg acaaatgtta
     1921 tgaaaatcgc gagtgggaat ttgcctatcg cgaaaatgat ccgctgggcg ggcacgatat
     1981 ttattcgatg agcaaggcgg cgacggaatt ggtcgcgcag gcgtggaacc gctcgttttt
     2041 caagccggac aaaaagcttg ggccggtcgt gactgcgcgc gcgggtaatg tcatcggtgg
     2101 cggcgattat gcggcggaca ggattttgcc ggattgcgtg cgttcgttga tcgcgaaaaa
     2161 accgattctc gtccgtaatc cacatgcggt gcggccatgg cagcatgtgc tggaatgtct
     2221 gagcggctat ttgtggctcg cggcgcggct ggaaggtgaa gacaaaatgt cacggttggc
     2281 tggcgctttt aatttcgggc cggaaccttc gtcacgtcag ccggtgcaaa agttggtgga
     2341 ggaagttttg acgcattggc cgggcaaatg ggtggatggt tcggacccga acagcccgca
     2401 cgaagcgacc ttgctgacgc tgtcgattga aaaggccggc gcgttgttgc aatggtcgcc
     2461 tgtgtgggat tttcaggaag cagtgggccg ttcgattgaa tggtaccatg agcgtcacgc
     2521 gttgaaaaac aaggagatgc tggcgctcag caacgcacaa attcacgcgt atgtggaagc
     2581 ggcaaagcgg aaaaaaatcg catgggcagt gtcatgagtt tcgatttcaa atgccgttcc
     2641 tgcaacagcc cgcgcggcgc gttgattttg gattt
//
LOCUS       DASZMM010000124         6175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1422036, whole genome shotgun sequence.
ACCESSION   DASZMM010000124 DASZMM010000000
VERSION     DASZMM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422036"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3285
                     /locus_tag="VG754_03085"
     CDS             <1..3285
                     /locus_tag="VG754_03085"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWF18219.1"
                     /translation="GSNLVGATLATLTFSNAQPGQSGNYSVIVSNTAGWTVSSNALLT
                     VNPVVTPPPATNITLIDVQFGATGSPNKTGGAAVGLTASDFWNFYNRDDGNGGFLTYG
                     TLNNLLLVDGSQTPVNLTVANAPGAWGNGSSDLMYANYLYPFNGGDITLTVSNLETGT
                     YDFYLYGHGDADNQNGVYQLSVNGQSYGTLADLNGPGWNNTNWQAGVQYVRFAAVPVT
                     TGQGIVITVQPGASSYAILAGLQIAQVTASSPVAPVVTNQPASQTVSQGDTVSFSVGV
                     SGSYPLTYQWQWGGSNLVGATLATLTFSNAQPGQSGNYSVIVSNTAGWTVSSNALLTV
                     NPVVIVPTANNQTVSVLEDSSVGIILTGTDPASNSLAYTVLSEPAHGALTGTAPNLSY
                     TPTPGYLGTDSFMFDVSNGQASSAAAVVSIDVVGQATSTLIDVDFGAGTSTSKHGYAA
                     IGESTNDMWNFYTRDGAQGGWLTGGSVANLAEVSGADTGAGLTVTNAPGAWGNGSTDP
                     MYATYLYPFDGGDIRLTVTNLSAGVYDFYLYGHGDADNQNSLFSLSVAGQNYGTLANT
                     TSSNWNSAIWQEGVQYVRFSGVSVGAGQTVGISVAPGVGGYAVLAGMQIAADTNFVAA
                     APAVTNQPASQTVGVGANVTLTAGVSGSHPLSFQWFFNSNTLAGATSATLTLGNIQLA
                     QAGTYALTVSNTVGATVSSNAVITVVPAANAQTVTLVENTSLGITLTGSASGADSLTF
                     TIVQPPTNGTLSGTAPALTYTPNADYAGSDSFTFQVSDGQNPSAPATVSINVLPESSG
                     PLIDIAFGAGTQTAKVGFAALGQTTNDFWNLYSRDDGHGGWRTFGLLTNIQFVNQIAS
                     SAVLTVSNAPGCWGNGSSDPMYATYIYPFDGGNITVTLINLDAGVYDFLIYGHAASDD
                     GNSTYHVDVDGQSYGDRTTTGPGWGALAWAENVQYVRFPTVQLNSGSVVTITVRPGGS
                     GYAILSGLQIERDLLNTGNSAPTNAPLVAKAQPMLFATVAKATVVAAPQLRVKAYDKG
                     GFAFTFTGEINRQHRIEYTDNPISGVWKPLAIVTPTQAVTTVTDPTSTTATKRYYRVV
                     ALPNTQ"
     gene            complement(3356..3601)
                     /locus_tag="VG754_03090"
     CDS             complement(3356..3601)
                     /locus_tag="VG754_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18220.1"
                     /translation="MSVTRGDRRRRELKLEDLPPSPARIQRRSHALGIGGARRLISID
                     THFHKPGADYLPSPFEIPVLLVPRQAIGYEVKVHDLN"
     gene            3724..4431
                     /locus_tag="VG754_03095"
     CDS             3724..4431
                     /locus_tag="VG754_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWF18221.1"
                     /translation="MIIYKLISQHLKHRDNEDFYELQASDAVHWMEKWGVPFRPGTTV
                     LDLGCGHGIFGACLAKRGCNVVFSDEANTLMPALAGSRYITCNLDQDDLSKLGTFDVV
                     ICSNVYEHLSKPDRFLENMAKVLKPGGFLFLSWTNWLSPWGGHEFSPFHYLGANHGHL
                     IYDKIFKRPRYHTPYQNLFPTYIGQTLKKIRRTSNLRVLRAFPRYYPEFSLIMKIPLL
                     REFVGWNCAMIIQKRQT"
     gene            complement(4516..>6175)
                     /locus_tag="VG754_03100"
     CDS             complement(4516..>6175)
                     /locus_tag="VG754_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18222.1"
                     /translation="TLEELRIAPAPLALAASGSPLLPRIRRLAGKSTINSDCTSWPVA
                     GLVALMLIVMVAIGLRGNRAVAQENKIAETNGKPAQSANQSSDASASNRLPEEKPAPN
                     DSNKPAFKTTATFGKGRQAILSKLESIQIDSIKYLNEPLTDVINNVAEIAKARDPEKI
                     GINLFIDRAMPINGNSIDPATGLPLKMDITQTMVTIDPALKHLRLMDVLDAITRGAGQ
                     PIQFKVIDYAVLFSFKETNEVALETRWFHVDPNTFRQGLQGVTGAPFGNGSAAGNGTG
                     QADAVTVPQTSVNAGAISLAGLQPLLRQFLSSAGMNMDTNNEGNMGKQFIYNDRKGIL
                     VVRATSNELENIGTALDVLNEIPAEVNIKTRFVEMDQPPDRRLLSSLLQTGTNQPLGA
                     VTGILTAKQFRAALDALERRNDVDELNAPEITTLLGRQVQVSVNDNSPKTNLLNLPQV
                     VDKNLREAGPILDVIPSVGSDEWNVNLKLTVNAPTWSTNSTSPSERQRNQVTSSIMVH
                     DGQTVMLAGMPIANSKPGSNKCLIVFVTPTLINPDGTRFHPKGE"
BASE COUNT         1315 a         1762 c         1729 g         1369 t
ORIGIN      
        1 ggcagcaatc tggtcggggc cactttggcg accttgacct tctccaacgc ccagcccggc
       61 cagtcgggca actactcggt gattgtcagc aacacggcgg gctggaccgt cagttccaac
      121 gccctgctca ccgtcaatcc ggtggtcacg ccgcctccgg ccaccaacat cacgctcatt
      181 gacgtgcagt ttggtgcgac cggttcgcca aacaaaaccg gcggggcggc ggttggcttg
      241 acagcctcgg atttctggaa cttttataat cgggatgacg gcaacggcgg gtttctcact
      301 tatggcacgc tcaacaacct cctgctggtg gacggcagcc agacgcccgt caacctcacg
      361 gtggccaacg cgccgggggc atgggggaat ggatcgagcg atctgatgta cgccaactac
      421 ctgtatccgt tcaatggcgg ggacatcaca ctgacggtga gcaacctgga aacgggcacc
      481 tatgactttt atctctatgg ccacggcgat gccgacaacc agaacggcgt ttatcaactg
      541 tctgtcaatg gccagagcta cgggaccctg gcggatttga acggccccgg ctggaacaac
      601 actaactggc aggcgggtgt gcagtacgtg cgctttgcgg cggtgccggt cacgaccggt
      661 cagggcattg tgatcacggt gcagcccggg gccagcagct acgcaatcct tgccggtctg
      721 cagatcgccc aggtgacggc cagctcgccg gtcgcgccgg tggtcaccaa ccagccggcc
      781 agccagacgg tttcgcaagg ggacacagtc tcgttctcag tgggcgtgag cggcagctat
      841 ccgctcacct accagtggca atggggcggc agcaatctgg tcggggccac tttggcgacc
      901 ttgaccttct ccaacgccca gcccggccag tcgggcaact actcggtgat tgtcagcaac
      961 acggcgggct ggaccgtcag ttccaacgcc ctgctcaccg tcaacccggt ggtcattgtg
     1021 cccacagcca acaaccaaac tgtgtccgtt ttggaagatt ccagtgttgg cattattttg
     1081 acaggtacag atccggccag caacagtctg gcttacaccg tgctttctga gcccgctcat
     1141 ggcgctctga cgggtacggc gcccaacctg tcttacacgc ccacgccggg atatttaggt
     1201 accgacagct tcatgtttga tgtcagcaat ggtcaggcat cttcggctgc tgcggtggtg
     1261 agcattgacg ttgtcgggca agccacctcg actttgattg acgtggactt cggcgcgggc
     1321 accagcacaa gcaagcacgg atacgccgcc attggagaat ccacgaatga catgtggaac
     1381 ttctataccc gcgacggcgc gcaaggcggt tggctgaccg gcggttcggt ggcaaatctg
     1441 gcggaagtct ccggggcaga tactggagcc ggcctgaccg tcacgaacgc gcccggagcc
     1501 tggggcaatg gttcaacgga tcccatgtat gccacatatc tttatccgtt tgatggcggc
     1561 gatattcgac tgacagtgac taatctgtcg gcaggcgttt acgattttta cctctatgga
     1621 catggcgacg ccgacaacca gaacagcctc ttcagcttga gtgtggccgg ccaaaattat
     1681 gggaccctgg ccaacaccac cagttccaat tggaacagcg caatctggca ggaaggcgtt
     1741 caatacgtga gattctcggg tgtaagcgtg ggcgcgggtc aaacagtggg aatcagcgtg
     1801 gctcccggag ttggaggcta cgcggtcctg gctgggatgc aaatcgcggc ggacaccaac
     1861 tttgtcgccg cagctccagc agtgacaaac cagccggcaa gccagacagt gggtgtcgga
     1921 gctaatgtca ccttgacggc cggagtcagc ggaagccatc cattgagttt tcaatggttc
     1981 ttcaacagca acacgctggc gggtgccacg agcgcgacgc tcacactcgg caacatccag
     2041 ctcgcacagg cggggaccta tgcgctgacg gtgagcaaca ccgtgggagc gactgtaagt
     2101 tccaatgctg tgatcacggt tgttcccgca gccaacgcgc agaccgtcac gctggtggaa
     2161 aacacctcgc ttggcatcac cctgactgga tcggcgtccg gtgccgattc attgacgttc
     2221 actatcgttc aacctcccac aaacggcact ttgagcggaa cggctcctgc gctgacctac
     2281 acgcccaacg cagattacgc cggctccgat tcattcacct tccaggtgag cgatggccag
     2341 aatccttccg ctccggcaac ggtaagcatc aacgttctgc cggaaagctc gggcccgttg
     2401 atcgatatcg cctttggagc cgggacgcaa acggcaaaag tgggattcgc ggccctgggc
     2461 cagacaacga atgatttctg gaacctttac agccgcgacg acgggcatgg aggttggcgg
     2521 acattcggac tgctgaccaa tatccaattc gtgaaccaga tcgcctccag cgcggtcctg
     2581 acggtaagca acgcgcccgg ttgctggggt aacggttcgt ccgacccgat gtatgcaacg
     2641 tatatttatc cattcgatgg cggcaatatc accgtgacgt tgattaatct ggatgcaggg
     2701 gtctatgatt tcctgattta cggtcatgca gccagcgatg atgggaacag cacctatcac
     2761 gttgacgtgg atggccagag ctacggcgac cgcactacca cggggcctgg atggggagcg
     2821 ctggcgtggg cggagaatgt gcaatacgta cggttcccga cggtgcagtt gaacagtggc
     2881 tccgtagtga cgattacggt tcgaccgggg ggcagcggct atgccattct ctcgggactg
     2941 cagattgagc gcgacctgct gaataccggc aacagcgcgc ccacgaatgc gcctctcgtt
     3001 gcgaaagcgc agccgatgtt gtttgcgaca gtggccaagg ccacggtcgt ggcggcacca
     3061 caactgcgcg tcaaagcgta cgacaagggc ggttttgcct tcacgtttac aggagaaatc
     3121 aatcgtcagc atcggattga gtacacggat aatccgattt ccggagtttg gaagccgttg
     3181 gccattgtga ctccgaccca ggccgtgaca accgtcacgg atcccacctc gacaactgca
     3241 accaagcgct actaccgcgt ggtagcgctg ccgaacaccc aataggccag cgggtggatt
     3301 tttggatgaa ggctgccggg atggaacaac cgttatttgc tccacctgct gggaattaat
     3361 ttaaatcgtg aaccttgacc tcgtatccaa tcgcctgtcg cgggacgagc aaaacaggaa
     3421 tttcaaacgg cgacggcaga taatccgccc ccggcttgtg aaaatgcgtg tctattgaga
     3481 tcagccgtct tgccccacca atacccagcg cgtgactgcg ccgttggatt cgggcgggtg
     3541 acggcggcaa gtcttcgagc tttaactccc gccgccgtcg gtcgccacgc gtaacggaca
     3601 cgccctgcct gcgcgaatag gcgtgcctat gaatgtttcc ttttttgcta actacgcgac
     3661 actcctaccc aatcggcttg ccattccaat caccctcaca taggctttat agacacgcag
     3721 tttatgatta tatacaagct gatttcccag cacctgaagc accgtgataa cgaagatttc
     3781 tatgaactcc aagcgtctga cgcggtgcac tggatggaaa aatggggtgt ccccttccga
     3841 ccgggaacaa ccgttctgga tctcggctgc ggacacggca tattcggagc ctgcctcgcc
     3901 aagcgcggct gcaacgtggt gttttcggat gaagccaata ccctgatgcc ggcactggcg
     3961 gggtcccgat atataacctg caacctcgac caggacgact tgtccaaatt gggcaccttc
     4021 gacgtggtga tctgctccaa tgtttacgaa catttgtcga agccggaccg tttcctcgaa
     4081 aacatggcca aggttttaaa gccaggcggc ttcttattcc tgagttggac caactggctt
     4141 tccccttggg gcgggcatga gttctcgccc tttcactatt tgggcgcgaa tcacgggcac
     4201 ctgatctacg acaagatttt caagcggccg agataccaca cgccttatca aaatttattt
     4261 ccgacctata tcggccagac actcaaaaaa attcgccgca cctcgaactt gcgagtgttg
     4321 cgggcgtttc ccaggtatta tccggaattc tcgctgatca tgaaaattcc actgctgcgg
     4381 gaatttgttg gctggaattg cgccatgatc atccagaagc ggcagacata gcccgaaatc
     4441 gtgccgttgc ctggggcagc cgtctaatgt ccgtccgcgg aatgcggatt ttacgatgtg
     4501 caatacccaa cagggtcact ctcccttggg atgaaaccgt gtgccatcgg gattgatgag
     4561 tgtgggagtg acaaatacga tgaggcactt gtttgagccg ggtttgctgt tcgcgattgg
     4621 catgcccgcc agcatcactg tttgtccatc atgaaccatg atgcttgaag tcacctggtt
     4681 cctctggcgt tcagaaggcg acgtggaatt tgtgctccaa gtcggtgcgt tcactgtcag
     4741 cttcaaatta acattccatt catcggagcc gaccgaagga atcacatcca aaatgggacc
     4801 ggcttccctc agatttttat ctactacttg cggcaggttg agcaagtttg tctttgggct
     4861 attgtcgttg acgctcactt gaacctggcg tcccaacaac gtggtgattt caggagcatt
     4921 caattcatcc acgtcattgc gtctttccaa agcgtcaagg gcggcgcgaa attgcttcgc
     4981 ggtcagaatg ccagtgacag cgccgagcgg ctgattggtg cctgtttgca aaagcgaaga
     5041 taaaagcctt cgatcgggtg gctgatccat ttccacaaat ctcgtcttga tgttcacctc
     5101 cgccggaatc tcattgagca cgtcgagagc agtgccgatg ttctccaact cattgctcgt
     5161 ggcgcgcaca acaagaattc ccttccgatc attataaatg aactgtttcc ccatgtttcc
     5221 ttcattgttc gtgtccatat tcatgccagc cgaagaaaga aattggcgga ggagcggttg
     5281 caaaccggcc aatgaaattg ccccggcgtt cacactggtt tgaggaactg tgaccgcgtc
     5341 ggcctgaccc gtgccatttc cggcggcaga accgttcccg aaaggcgcgc cagtcacccc
     5401 ttgcaatcct tgccgaaatg tattcgggtc cacatggaac cacctggttt caagcgccac
     5461 ctcgttcgtc tccttgaagc tgaagagcac tgcgtaatca atgactttga attgaatcgg
     5521 ctggccagcg ccgcgcgtga tggcatccag cacgtccatc aatctcaaat gcttcaacgc
     5581 cggatcaatg gtgaccattg tttgtgtgat gtccatcttc aagggaaggc cggtcgcggg
     5641 gtcaatcgag ttgccattaa tcggcatcgc ccggtcgatg aataaattga tgccaatctt
     5701 ctccggatcg cgcgccttgg cgatctcggc gacattattt atgacgtcgg tcagcggctc
     5761 gttgagatat tttatggaat cgatctggat gctttccaat ttggacaaga tggcctggcg
     5821 gccctttcca aaagtggctg tcgttttgaa agccggtttg tttgaatcat tcggcgcggg
     5881 tttttcctcc ggcaaacggt tcgatgccga agcgtcgctt gattggttcg cgctttgtgc
     5941 cggttttcca tttgtctccg cgattttatt ttcctgcgcc acggcgcgat tgccgcgcag
     6001 gccaattgcc accatgacga tcagcatcaa cgcgaccaat ccggcaaccg gccatgaagt
     6061 gcaatcgctg ttgattgtgg attttccggc cagccgtcga atccgcggca gaagcggtga
     6121 accggacgcc gccaatgcga gcggcgcggg cgcgatgcgc aattcttcca atgtc
//
LOCUS       DASZMM010000125         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_314350, whole genome shotgun sequence.
ACCESSION   DASZMM010000125 DASZMM010000000
VERSION     DASZMM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314350"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..846)
                     /locus_tag="VG754_03105"
     CDS             complement(22..846)
                     /locus_tag="VG754_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF18223.1"
                     /translation="MNLEDHLGDIIRKGRKAANVEPDAAAKAAGLSIVELSALEESGK
                     VIGNPNFAALATLLALHPTKLANIAKGWLPSEKDLSQWRELRVFQTEQNGIVVNSYLV
                     WDEVSREAAVFDTGWNAQSAIAAIEENQLQLRHIFLTHTHEDHIAGMAGLREKFPKAH
                     IHTNSKSAPPQQRNRANDFIHLGSLRITNRETPGHAEDGVTYIIGTWPEDAPHVAIVG
                     DSIFAGSIGTGFQSWDLAKQKVREQIFSLPPDTLICSGHGPLTTVGEEKANNPFFS"
     gene            1712..>3195
                     /locus_tag="VG754_03110"
     CDS             1712..>3195
                     /locus_tag="VG754_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18224.1"
                     /translation="MKAPLHIIHVEDNPYDADLARALLNNAGIACEATCVKTPEELQE
                     ALNRPELDLILSDFSMPKFDGLSALRMSRKSRPEVPFIFLSGTIGEEVAIESIKEGAT
                     DYVIKDRMSRLPFCVRRAVREAAEASERRRMEERIREQAALLDRAQDAICLNDMSQQI
                     LFWNRSAERLYGWKAWEAMGRNCNQLLFQDDLTAPSEALRNLIRKGEWQGELHQITKD
                     RRRIIVESRWTLLRNEGGEPKSILIINTDITEKKQIEAQFLRTQRLETIGALAGGIAH
                     DLNNALTPILMAGSLLRKELPTGDGARLLEMMIGSAQRGAEMVKQILSFSRGVGGTPV
                     PLQVQSLVNEMAQLARKTFSRSIKIEANIAEDLPEVTGNATQLHQVLLNLCVNARDAM
                     PQGGTLTIETSSITLKGATLPDGRRSDPGVYVLIKVSDTGHGIPPEIREKIFEPFFTT
                     KEMGNGTGLGLSTVMGIVKTHGGFLDLTSQVGQGTTFKVYLPSK"
BASE COUNT          709 a          846 c          909 g          731 t
ORIGIN      
        1 gtctcctacc ttaacgggcc gttagctgaa aaacggattg ttcgcctttt cttcgccgac
       61 ggttgtcaaa gggccgtggc cggagcagat caatgtatcg gggggcaatg agaaaatttg
      121 ttcgcgcact ttttgtttgg cgaggtccca agactgaaag ccggtgccga ttgagccggc
      181 gaaaatcgaa tcgcccacga ttgcgacgtg cggggcgtct tcgggccagg tgccaatgat
      241 gtaagtgacg ccgtcctcgg catggccggg tgtttcgcga ttggtgattc gcaggctgcc
      301 gagatggatg aaatcgttgg cgcgattgcg ctgttgcggc ggcgcgcttt tggaattggt
      361 gtggatgtgc gccttgggaa atttttcgcg caaacccgcc atgcccgcga tgtggtcctc
      421 gtgcgtgtgc gtgagaaaaa tgtggcgcaa ctgaagctgg ttttcctcga tggccgcaat
      481 ggcggattgc gcgttccatc cggtgtcaaa caccgccgcc tcgcgcgaca cttcgtccca
      541 gacgagatac gaattcacca cgatgccgtt ttgttcggtc tggaagacgc gcaattcgcg
      601 ccattggctt aaatcttttt cgctgggcaa ccatcctttc gcgatgttcg ccagttttgt
      661 tggatgcaga gcaagcagcg ttgccaacgc ggcgaaattg ggattgccaa tgactttgcc
      721 ggactcttcc aacgcgctca attcgacgat gctcaagccg gccgcttttg cagccgcgtc
      781 cggttccacg ttcgcagctt tgcgaccttt gcgaatgatg tcgcccaaat gatcttccaa
      841 attcatgtct ggtcatggaa ccagaaaggc cggacgggga aaaggcagaa atggagcgca
      901 ggggactgga tgtgcattga cattattctg gagcggggac tggaaagtcc ccgcaacccg
      961 caggctggaa agcctgcgct acgtagcgca gacagtcttg tctgcgggtt caggcgactt
     1021 tccagtcgcc tgaagttctg ctctcacaat actcgatcaa gtattgaggg gtaatgctcg
     1081 ttctctttcc agtagcgtcc ccatagtggg cgtcaagcca gtcggaaccg gcgattttat
     1141 tggcaaatcc agatgaacga cagaaaatga gattaaggac ttctttatta ggaaatcatg
     1201 aaatcaggaa caaaattctt gaggaaacat tgttttttcc tgctttccta attctatttt
     1261 cccacgggat ggctctgttt tccatccaac acttcatcgc atcttccact actccaactt
     1321 ttctcctatg tcactgaaag tctgcatgtt agacattttg taggcatttt gtaagttgcc
     1381 attattcagt tttggtcgta cattatttcc gatgttgagt cccagccgag ccatgctcca
     1441 tcctatggcg aaaactcgtc gtattctgct ggttgaagac agcgcacatg tcgcattgac
     1501 attggccacg cgccgcgcca gccgcattgt ccatgaagac gccgtgaggc gcgacgatgt
     1561 cttttccaaa gcagcgcttt tcggtcgcat aattcacatt ggtcaggttt ctgctttcat
     1621 ttattcgcga aattcaccgc aaatcatttt tttacacccg gcgcatctcg ttgactgaat
     1681 ggaacctcga actgagatga gagattttgt tatgaaagct ccattgcaca taatccacgt
     1741 ggaggacaat ccctatgacg ccgacctcgc gcgcgcattg ttgaacaacg ccggcatcgc
     1801 ctgcgaagcg acctgcgtca agacaccgga agagcttcaa gaagccctga accggcccga
     1861 gctggacctg atcctttcgg atttttccat gccgaaattt gacggcctga gcgcattgcg
     1921 gatgtcacgc aaaagcaggc ccgaagtgcc gttcattttt ctgtcgggga ccatcggcga
     1981 ggaagtggcc atcgaatcca tcaaggaggg cgccaccgat tatgtcatca aggaccggat
     2041 gtcgcgattg ccgttttgtg tgcggcgcgc ggtgcgtgaa gccgcggaag cgagcgaacg
     2101 ccgccgcatg gaagagcgca tccgcgagca ggcggcgttg ctggaccgcg cgcaagacgc
     2161 gatttgtttg aacgacatgt cgcaacaaat ccttttttgg aaccggagcg ccgagcgttt
     2221 gtatggctgg aaggcatggg aagcgatggg gcggaattgc aaccagttgc tttttcaaga
     2281 cgatttgacc gccccgtcgg aggcgttgcg caacctgatt cgcaagggcg aatggcaggg
     2341 ggaattgcac cagataacca aggacagacg ccggatcatc gtggaaagcc gctggacgct
     2401 gttgcgcaat gaaggcggcg agccgaaatc catcctcatc atcaacaccg acatcacgga
     2461 gaaaaaacag atcgaggcgc aatttcttcg gacgcaacgg ctcgaaacca tcggcgcgct
     2521 cgccggcgga attgcgcatg acctgaacaa cgcgctcacg ccaatcttga tggcgggcag
     2581 cctgctgcgg aaggaactcc caacgggaga tggcgcgcgt ttgcttgaaa tgatgatcgg
     2641 cagcgcgcaa cgcggcgcgg aaatggtgaa gcagatactt tcgttttcgc gcggcgtggg
     2701 cggcacgcca gtgcctttgc aagtccaatc cctggtcaac gaaatggcgc agcttgcgag
     2761 aaaaactttt tcgcgctcca tcaaaattga agccaacatc gccgaggatc tgcccgaagt
     2821 gaccggcaac gcgacgcaac tgcaccaggt tcttttgaat ttgtgcgtca acgcgcgcga
     2881 cgccatgccg caaggcggca cgttgaccat cgagacgtcg agcatcacgc tcaaaggcgc
     2941 gacgttgccg gacggccgca ggtcggaccc gggagtttac gtcttgatca aggtgtctga
     3001 caccgggcac ggcatcccgc cggagattcg tgagaagatt tttgagcctt ttttcaccac
     3061 aaaggaaatg ggcaatggga ccggactcgg gctttccacc gtcatgggca tcgtcaagac
     3121 gcacggcggt ttcctggatt tgaccagcca agtggggcag gggacgacgt tcaaggttta
     3181 tctgccctcg aaaaa
//
LOCUS       DASZMM010000126         5976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2521636, whole genome shotgun sequence.
ACCESSION   DASZMM010000126 DASZMM010000000
VERSION     DASZMM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521636"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VG754_03115"
     CDS             <1..296
                     /locus_tag="VG754_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18225.1"
                     /translation="PNPAVSASQIFSKTSAKNRPSQDLVLDNPARRSLCMASSLFRRS
                     TRLAPDWHGYKNTLFERRLSSAGLREGPRLAVVIEISQGPIAAQGKFARKTSH"
     gene            complement(711..1958)
                     /locus_tag="VG754_03120"
     CDS             complement(711..1958)
                     /locus_tag="VG754_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HWF18226.1"
                     /translation="MPTIAILGTMDTKGEEHAFVAEQIRRRGHQVLVIDVGTLGEPLL
                     KPDVSRQQVASAAGADLEAIVRKRDRGEAIAVMSKGAPIVLVELAEAKKIDGVISLGG
                     GGGTAIGTAAMRALPIGFPKLMVSTLASGNTAQYVGVKDIVMFPSIVDVAGLNRISRQ
                     ILTRAAGAICGMVEAVAETDTVKGGHRTEDKPIIVASMFGNTTDCVQAARKILEGAGY
                     EVLVFHATGTGGRTMESLIETGLVAGVLDITTTEWADELVGGFLSAGPTRLEAAAKHG
                     TPAIVVPGCLDMVNFHGPESVPSKFKGRNFYQHNPQVTLMRTTPEECAQLGKIIAEKL
                     NASRGPVTVLLPLKGVSVISAPGQKFYDASADAALFDALKKNLRTGIKVVEMDCNIND
                     ARFAKACAKELERNIALGESNKS"
     gene            complement(1996..2676)
                     /locus_tag="VG754_03125"
     CDS             complement(1996..2676)
                     /locus_tag="VG754_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18227.1"
                     /translation="MNFKFPDENRTFREFFPDPKLRHTMHYNFAHRFLPQYIHRNPYA
                     FFSYIFRRDAPGLPMEPNRFLHSRWMAFEENIKGTMWQNVNSFRRVTDMTMSLHEAAG
                     RPVALVQMPNPEQPAEAFFVAATLLASPAPEGWSRDVLARVFTLEALSPLFPESRTHG
                     MFCEWLKNGRHANYGIGYTPEHGAFLQGVTVALQVELPESASFTPSEAGGLGAISANI
                     PVRPPTKS"
     gene            2890..3591
                     /locus_tag="VG754_03130"
     CDS             2890..3591
                     /locus_tag="VG754_03130"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HWF18228.1"
                     /translation="MLTLVIATRNAHKAGEIRAILSDRFRYLTLADFPDAPAVVEDAA
                     TFNGNATKKAVELSQWLASHHGEKIKANSPLFVLADDSGLEADALHGAPGVLSARFAA
                     LDTGQSGNTPDAGNNAKLLRLLKDVPAERRTARFRCVLALTPVSESSSENASPVCSAN
                     EAELQTQLFDGACEGHIAFGPSGKGGFGYDPLFIPEGHEQSFAELGEATKNQLSHRSK
                     ALAKLRNYLDAMTAR"
     gene            complement(3602..4516)
                     /locus_tag="VG754_03135"
     CDS             complement(3602..4516)
                     /locus_tag="VG754_03135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWF18229.1"
                     /translation="MKILRRAFLLFIGLLFTQACDAGTLVQFHIFFGVNNYVGDVDVE
                     LYNQDKPVTVNNFLRLIEAGAYNDSFFHRLQPGFVLQGGGYFAFNPFATNVIAPPYAN
                     LGLVGNFGSITNEFKVGKFYSNTNWTIAMAKTSDPNSANSQFFFNLTNNASSLDNTNN
                     IGGFTVFGHTLRGINLLNQFNGLSYGSGLFNLTNNYGVNGALFNQLPANFAGTNPPPY
                     DDLVYFTITILSAQVNVKTNGARQISWEAISGMTNRVEYATNLPPTWQVLTNIYPTNA
                     VATNVIDSTTNKTRFYRVHVLYPNTPPS"
     gene            complement(4552..5379)
                     /locus_tag="VG754_03140"
     CDS             complement(4552..5379)
                     /locus_tag="VG754_03140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWF18230.1"
                     /translation="MNSSEQRHALVCAVKAARAAGSLMRKNLRAVKRINSKTQHDIKL
                     ELDVRCQKLIERTLAAEFPKIALLGEEGISGNPDAAQRWVVDPIDGTVNFTYGIPHAC
                     VSIALQVQSSKFKVQSQKSNNDQKQEDQETIVGVVYDPFCDELWTAIRGQPARLNGKI
                     IRVSDRKSLRETIVAIGFAKTHASLEATLPYFNQLVRRTRKVRIMGSAALAMVYVASG
                     RFDAYVERGIRLWDIAAGGLIVECAGGEFWRRPIPGDYAYEMVTSNGFLRKKLESIG"
     gene            complement(5429..>5976)
                     /locus_tag="VG754_03145"
     CDS             complement(5429..>5976)
                     /locus_tag="VG754_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWF18231.1"
                     /translation="LKAKKIYDWLSDNLQYSYALEYSTIRNLSDYTRSHCYGDCGQQA
                     LFFITLCRYNGVPARWQAGWNLFPRATSNHDWAEMYLAPYGWMPVDPYMGNYAMRYTS
                     TLTPQQKIEMRDFYFGGLDQYRMAANSDHNQPLNPPKESMRSDDVDFQRGELEHNGTN
                     IYFDKFSWALHYKVVPATSVP"
BASE COUNT         1357 a         1657 c         1538 g         1424 t
ORIGIN      
        1 gcccgaatcc ggctgtttca gcttcgcaaa tattttccaa aacttcggcc aaaaatcgtc
       61 cgtcacagga tttagttttg gataacccgg cacgacgcag tttgtgtatg gccagctccc
      121 tttttcgtcg atcaacccgg ctcgcaccgg attggcacgg atataaaaac acactttttg
      181 aaaggcgcct ctcttccgct ggtctgcgcg aaggacctcg tcttgcggtt gtgattgaaa
      241 tttcacaggg gccaatcgcg gctcaaggta agttcgcaag aaagacatcc cattgaattg
      301 atctgaatca agccgcagtc ccatccagac gaaatgaacg tggtctggca tgagacaata
      361 aatgggacaa gccaaacctt cacgagcggc ggcatggaac atgagttctc gaaagtactg
      421 atgaagtggc aggtttagcc aacctttgct tttgtcatgc aatgtcaaag tccagaagac
      481 aacggcgtcg ccttgataat gctcacggga aagacgcggc agataaaatg tgcgattgca
      541 ggaatctcct gtttggcttg cagacagttt tcgttccatg ctggatgtca gagactcctc
      601 acatcgtctc ctaccatgtt gtggtggaaa cggattgatt tatcaatcac gatttcgccg
      661 aacccgtggg accattacgc gaaaaatatc agagactcgc acgtcgtccc ctacgattta
      721 ttggattcgc ccagcgctat attacgctcc aattccttcg cgcacgcttt ggcaaaacgc
      781 gcgtcattga tgttgcaatc catttcaacc accttgattc ccgttcgcag attcttcttt
      841 aacgcatcga acaacgccgc gtccgcagac gcgtcataaa atttttgtcc cggcgcgctg
      901 atgacgctga cgcctttgag cggcagcaac acggtgaccg ggccgcgcga cgcattcaat
      961 ttttccgcga tgattttgcc aagctgcgcg cattcttccg gcgtggtgcg catcaacgtg
     1021 acctgtggat tgtgctgata aaaatttcgg cctttgaatt ttgaaggcac gctttccggc
     1081 ccatgaaaat tgaccatgtc cagacaaccg ggaaccacga tggccggggt gccgtgtttc
     1141 gccgcggctt caagccgcgt aggaccggcg ctgagaaaac cgcccaccaa ttcatccgcc
     1201 cattcagtcg tggtgatgtc cagcacgccg gcaactaaac ccgtttcaat cagcgattcc
     1261 atcgtgcggc cgcctgtgcc ggtcgcgtgg aacacgagaa cttcataacc cgcgccttcc
     1321 aagattttcc gcgccgcctg cacgcaatcc gtcgtgttcc cgaacatgct cgccacgatg
     1381 atgggtttgt cttcggttcg gtgtccaccc tttacggtgt cggtttccgc aaccgcctcc
     1441 accatcccgc aaatcgctcc cgctgcgcgc gtcaaaatct gccgcgaaat ccggttcaac
     1501 cccgccacat ccacgatgct cgggaacatc acgatgtcct tcaccccgac atattgcgcc
     1561 gtgttgccgc tcgcgagcgt cgagaccatc aatttcggaa aaccgatcgg caacgcgcgc
     1621 atcgcagccg ttccgatggc cgttccgccc ccgccgccca aggaaatgac gccgtcaatt
     1681 ttttttgcct ccgccagctc cactaataca attggcgcgc ctttcgacat caccgcaatc
     1741 gcttccccgc gatcccgttt gcggacgatt gcctccaaat ccgcgcccgc cgcagatgcc
     1801 acttgctgcc gcgaaacatc cggtttcagc aacggttcgc caagcgtgcc gacatcaatg
     1861 accagcactt gatgcccgcg ccttcggatt tgttccgcca caaacgcgtg ttcctcgccc
     1921 ttggtgtcca tggtgccgag aatcgcgatg gttggcatag ctgtttggcg taattgaagg
     1981 atgcaactcc aaagttcaag actttgtcgg tggtcgaacg ggtatgttgg ccgaaatggc
     2041 tcccaaccct ccagcttcgg atggtgtgaa gctggcgctt tctggcaact caacctgcaa
     2101 cgcgaccgtc accccctgaa ggaatgcccc atgctccggg gtatagccga tgccgtagtt
     2161 cgcatgtctc ccatttttca gccattcgca aaacatgccg tgcgtcctcg attccggaaa
     2221 taaaggggac agagcttcca gtgtgaagac ccttgccagg acatcccgag accaaccttc
     2281 aggtgcgggt gaagcgagca atgtcgccgc cacaaagaac gcctcggcgg gttgttcagg
     2341 atttggcatc tgcacaagcg cgaccggcct ccctgccgcc tcatgaagag acatcgtcat
     2401 gtcggtaacc cggcggaacg aatttacgtt ttgccacatc gttcccttga tgttttcctc
     2461 gaatgccatc catcggctgt gaaggaatcg gttcggttcc atcggtaagc cgggtgcatc
     2521 tcgccggaaa atataggaaa agaaagcgta agggtttcga tggatatact ggggcaggaa
     2581 ccggtgcgcg aaattgtaat gcatcgtgtg ccgcagtttg ggatcgggaa agaattccct
     2641 gaaagtgcgg ttttcgtctg gaaacttgaa gttcatagca cgtgttcaaa ccaccccaaa
     2701 tcaacattat ccatcggatt tgtttatcgg catcatgtca agctttatgg ttttgggaat
     2761 cggtagagta agcgaagtgg gaggaggcgg gttgaaccga ccgtaattgt cagtttcgcg
     2821 ctgccacttc gcttactcta tagtttgaat cttatcgtat gagccgatat ttcgtgggat
     2881 tattcgcacg tgctgacact cgtgatcgcc acgcgcaacg cccacaaggc cggggaaatc
     2941 cgcgcaatcc tttccgaccg ttttcgatat ttgacattgg cagattttcc tgatgctccg
     3001 gcggtcgttg aagatgccgc cacgttcaat ggcaatgcga ctaaaaaagc cgtcgaactt
     3061 tcacaatggc tggcttctca ccacggtgaa aaaatcaaag caaattcccc gttgtttgtg
     3121 cttgccgatg attcgggttt ggaagcagat gcgcttcacg gcgcgccggg tgttctttcc
     3181 gcgcgtttcg cagctttgga taccggccaa tccggcaata cgcccgatgc cggcaacaac
     3241 gccaaactgt tgcgccttct gaaggatgtt cccgccgagc gacgcaccgc cagatttcgt
     3301 tgcgtgcttg cactcacacc tgtttcggaa agttcgtcag aaaacgcttc ccctgtttgc
     3361 tctgcgaatg aagcggaact ccaaactcaa ttgttcgacg gcgcgtgtga aggccatatc
     3421 gcattcgggc catctggcaa ggggggattt ggttatgatc cgcttttcat tccagaaggc
     3481 catgaacaga gcttcgccga actcggtgaa gcgactaaaa accaactcag ccatcgttcg
     3541 aaggcgctgg ccaagctgcg caattatttg gatgccatga ccgcgcggtg aactgtttga
     3601 gtcagcttgg cggcgtattt ggatacaaaa cgtgcacgcg atagaaccgc gtcttgttcg
     3661 tggtggaatc aatcacattc gtcgccaccg cgttggttgg ataaatattt gtcaaaactt
     3721 gccaggtggg tggcaggttg gtcgcgtatt ccacgcgatt ggtcattccg ctgatggcct
     3781 cccaactgat ctgccgcgcc ccgttcgtct tcacattcac ctgcgcgctg agaattgtga
     3841 tggtgaaata aaccagatca tcataaggcg gagggtttgt accggcaaaa ttggccggca
     3901 actggttgaa aagcgctcca tttacgccgt aattattggt caggttgaac aaaccgctgc
     3961 cataagaaag tccattaaat tgattcagaa gattgatccc gcgaagtgtg tggccaaaca
     4021 cggtgaaacc gccgatgttg ttcgtgttat caagcgatga tgcattgttg gtgagattga
     4081 aaaagaattg tgaattcgcg gaattgggat cgctcgtctt ggccatggcg atggtccagt
     4141 tggtgttgct gtaaaacttt cccaccttga attcgttggt gatgctccca aaattgccga
     4201 ccagcccgag gttcgcgtag ggcggcgcga tgacgttcgt ggcgaacgga ttgaatgcga
     4261 aataaccgcc accttgcagc acaaatcccg gttgcaaccg gtgaaagaaa gaatcgttgt
     4321 aagcgcctgc ctcgatcagc cggagaaaat tgttgaccgt gaccggcttg tcctggttgt
     4381 aaagttccac gtccacatcg cccacgtaat tatttacccc gaaaaaaatg tgaaactgca
     4441 ccaatgtccc cgcatcgcag gcttgcgtga acaggaggcc gatgaacaaa agaaaagcgc
     4501 gccgcaaaat tttcatgccg ttgaaaaaac cagcatggca tgtaactgga atcacccgat
     4561 ggactcaagc tttttccgca gaaaaccgtt gctggtgacc atttcgtaag cgtaatcgcc
     4621 gggaatgggc ctgcgccaga attcgccgcc cgcacattcc acgatcagcc cgcctgccgc
     4681 gatgtcccaa agccttatgc ctcgctcgac ataagcatcg aaacgcccgc tggccacata
     4741 caccatcgca agcgccgcgg aacccatgat gcggaccttg cgcgtgcgcc gcaccaattg
     4801 gttgaaatag ggcagggtgg cttcgagact cgcatgagtc ttggcaaatc caatcgcgac
     4861 aatcgtctcg cgcaaagact tccggtcgct gacgcgaatg attttgccat tcaaccgcgc
     4921 cggctgcccg cgaatcgcag tccatagttc gtcacaaaac ggatcgtaaa cgacgccaac
     4981 tatcgtttcc tgatcttcct gcttttggtc gttatttgat ttttgacttt gaaccttaaa
     5041 ctttgaactc tgaacttgca gcgcgatgga cacgcacgcg tgcggaattc cgtaagtgaa
     5101 attcaccgtg ccatcaatcg gatcaaccac ccagcgttgt gccgcatcgg gatttcctga
     5161 aataccttcc tcgcccaaca acgcaatctt tggaaattcc gcagccaatg ttctctcgat
     5221 caatttctgg cagcgaacat cgagttccag cttgatgtcg tgctgcgtct tggaattgat
     5281 gcgcttcacc gcccgcagat ttttgcgcat caagctgccg gcggcgcgcg cggcttttac
     5341 ggcacagacc aaagcgtgtc tttgttcaga tgaattcatt tgcgattaga aaacttggtt
     5401 tcggcggcgc ggcagctctg cccaattatc acggcaccga cgtcgcaggc accactttgt
     5461 agtgcaaagc ccaggaaaat ttgtcgaaat agatattggt gccattgtgc tcgagttcgc
     5521 cgcgctgaaa atccacgtcg tcggaacgca tggactcttt cggcgggttc aatggttgat
     5581 tgtggtcgct gttcgcggcc atgcgatatt ggtccaagcc gccgaaataa aaatcgcgca
     5641 tctcaatttt ctgctgcggt gtcagcgtgc tggtgtagcg catcgcatag tttcccatgt
     5701 aagggtccac cggcatccag ccgtagggcg cgagatacat ctccgcccaa tcatgattgc
     5761 tcgttgcgcg cggaaacaaa ttccaaccag cctgccagcg cgcgggcacg ccgttgtagc
     5821 ggcaaagcgt aatgaagaat agcgcttgtt ggccgcaatc gccgtagcaa tgcgaacggg
     5881 tgtaatcact gaggttgcgg atggtggaat attcgagcgc gtaactgtat tgcaggttgt
     5941 cggaaagcca gtcgtagatt ttcttcgctt tcagca
//
LOCUS       DASZMM010000127         4297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1422862, whole genome shotgun sequence.
ACCESSION   DASZMM010000127 DASZMM010000000
VERSION     DASZMM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422862"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VG754_03150"
     CDS             <1..464
                     /locus_tag="VG754_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18232.1"
                     /translation="FLAFAVSTALAVNSPAQDTPSKSTNAPVATRSGKQADLASTTAK
                     FGSVAKTDDAYKSAIDAHALDDALKLAGKESAFKGMVTKIFEPRNVALINFDADYHSA
                     ITAVVQGTNFSKFPALTNLVGKNVLVTGTFTNYQGRPEIVLTNAQQIKLAE"
     gene            495..1181
                     /locus_tag="VG754_03155"
     CDS             495..1181
                     /locus_tag="VG754_03155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWF18233.1"
                     /translation="MRVLNKYSALLLDMNGTFMFGHDRFGEAEDFHRTYRSLGGNTLS
                     KHEVKRCMRKCFDSLDRDSTNPAVYDDFPSLAEAFQRYSNPPESELPLLQRVFALHEA
                     GTVSEAHASLLQRLARTHRLALVSNIWSPKQPWLDEFKRAGLGGVFQHQVFSSDFRSI
                     KPSPVLFREALRGLDKRPQDCLFIGDSLRCDMEGACRVGIATAWLSPKTEQPESVGYL
                     ISNLAQIEEL"
     gene            1259..1453
                     /locus_tag="VG754_03160"
     CDS             1259..1453
                     /locus_tag="VG754_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18234.1"
                     /translation="MSTAQEIEAAIRALPANEREKLVRDLPVILPELNGDFEWARIIS
                     DPRPRASLSALGDEIEARFL"
     gene            complement(1467..2975)
                     /locus_tag="VG754_03165"
     CDS             complement(1467..2975)
                     /locus_tag="VG754_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWF18235.1"
                     /translation="MKSELSSERASEKPSGHPSINAGLLPALGLFTTIMMVVGGVIGS
                     GIFRKPGLMAGQVGSPGLLLMVWLLAGVLTLFGALTNAEVASVIPETGGQYVYFERMF
                     GSFFAYLYGWAVFAVIQTGSIAAVAYVFAEYAGKLVQLPEFSPEVSAFTIHFPFIGDV
                     APLADIGVKGLAAVLIVGLTAVNYIGVKLGGVVQNIFTIAKVAAMALLFMGAFFLPTG
                     GAAANLTTSSTVIHPAGFALLLAIVAAMQGAFWAYDGWNKITYIAGEVTHPQRNIPLG
                     LGIGMLIVMVIYMLMNVAYAYVLPVDVMAHSKLVAADVAEKCFHGGARWIAVAVMIST
                     FGTANATILASARVYFSMSRRNVFPKFLGTAHPKFHTPAASLIVQGIWSVLLLFSGTF
                     DTLTDTLIFVSWVFYAAGAYGVFVLRRQMPGAARPYKVPGYPIVPIVFIVFATIYLGF
                     SIYNDIVDYQAAVAAGKHAIINSAFGMVLVFIGTPIYWFYRKMNHQDTKTQS"
     gene            complement(3074..4153)
                     /locus_tag="VG754_03170"
     CDS             complement(3074..4153)
                     /locus_tag="VG754_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWF18236.1"
                     /translation="MGAKERGFHKAMLSIYEKSRDQIGYRPSRFLAKVRRDGGLSSAK
                     YWLKRPVVSKGFERLRTAGKLNLSVEALVLKPEYKKLFTDAEREIALQRLKDHGWHQE
                     EEPRKEKQVTSRCFWIFQAIPKQYDLKKELRLRKIDHWLVRHFRGLMKPGDKVIFWQA
                     GKESGIYGVGELTDKPYLDENSDWRVETVYTSHLNPPLLKKQLLKDSVLKNLRILKPP
                     FQGTNFKATKEQWNALGVIDDIFHADEIEAAQRFAEGATVQRTVNAYERNPHAKRVCL
                     DHYGYKCSVCLINYAEKYGEIGHACIHVHHLKMLSGIGREYDVDPIIDLRPVCPNCHA
                     MIHSRSPMMSIDELRDLVLKNSLLK"
BASE COUNT         1072 a         1212 c         1003 g         1010 t
ORIGIN      
        1 ttttcctcgc gttcgcagtt tcaaccgcgc tcgccgtcaa ctcgcccgcg caggatactc
       61 cctcaaaatc caccaatgcg cctgtggcaa cgcgttcggg caaacaagcc gaccttgcct
      121 ccacgactgc caaattcggc agcgtggcaa aaaccgacga tgcatacaaa tccgccattg
      181 acgcccatgc gcttgatgac gcgctcaaac tggctggcaa agaaagcgca ttcaaaggaa
      241 tggtcacaaa gattttcgag ccgcgcaacg tggcgctcat caattttgac gcggactatc
      301 acagcgccat cacagcagtc gttcagggga ccaacttctc caaattccct gcgctcacga
      361 atctggtcgg caaaaacgtc ctcgtcaccg gcacattcac caactatcag ggccgtccgg
      421 aaattgtgct gaccaacgcg caacagatca aattggcgga atgagacgct acaatttgcg
      481 tcatcagctt ctggatgaga gttcttaata aatactcagc gctgctgctc gacatgaacg
      541 gcacattcat gttcgggcac gatagatttg gcgaggccga ggattttcat cgcacttatc
      601 gttctctggg cgggaacacg ctttccaagc acgaggttaa gcggtgcatg cgaaaatgtt
      661 tcgacagtct ggatcgggat tccacaaacc cggctgttta tgatgatttt cccagtctcg
      721 ctgaagcttt tcagcgttac tcgaatccgc cggagtccga attgccattg ctgcagcgtg
      781 ttttcgcctt gcacgaagcg ggtacggtgt ccgaagccca cgcttcgctg ttgcaacgac
      841 ttgcccgaac gcatcgcctg gccttggtct caaatatctg gtcgcctaaa caaccctggt
      901 tggatgagtt taaacgcgcc ggtctcggcg gtgtcttcca gcatcaggtt ttttcttcgg
      961 actttcgcag catcaaaccg tcaccggtcc tctttcgtga agctttgcgc ggactggata
     1021 agcgcccgca agactgtcta ttcatcggag acagtctgcg ttgcgacatg gaaggtgcct
     1081 gccgcgtggg aattgcaaca gcctggcttt cgcccaaaac ggagcagcct gaatcggtgg
     1141 gttacttgat ttccaacctt gcgcaaatcg aagaattata aacacacggc aaacgaaatg
     1201 gattgcgtgc aaagcactcc ctgctagttt caaacatagt taaagttaag ttttgaccgt
     1261 gagcaccgca caagaaatcg aagcggcaat tcgcgcgttg ccagccaacg aacgcgaaaa
     1321 gctcgtgcgg gatttgcctg ttattttgcc ggagttgaac ggcgattttg aatgggcgcg
     1381 tatcatctca gatcctcgtc cgagggcttc tttatcagct cttggagatg aaatcgaagc
     1441 acgattcttg taactgctaa tttttcctaa ctttgtgtct tggtgtcttg gtggttcatt
     1501 ttccgataaa accaataaat cggcgtgcca atgaaaacca gcaccatgcc aaaggcggag
     1561 ttgatgatgg catgtttgcc cgcggcgact gcggcttgat aatccacaat atcgttgtag
     1621 atgctgaacc cgagataaat ggtcgcaaaa acaataaaca ctatcggcac gattggataa
     1681 ccgggaactt tgtacgggcg cgccgcaccg ggcatttgcc ggcgcaacac aaacacgcca
     1741 tacgcgcccg ccgcataaaa aacccagctc acgaaaatca acgtgtccgt cagcgtgtcg
     1801 aacgttccgc tgaacagcaa cagcacgctc caaatcccct gcacaatcag cgacgcggcc
     1861 ggcgtgtgaa atttcggatg cgccgttccc agaaatttcg ggaaaacgtt ccggcgcgac
     1921 atcgaaaaat aaacccgcgc gctggcaagg atggttgcat tggccgtgcc gaaggttgag
     1981 atcatcaccg ccaccgctat ccaacgcgcg ccgccgtgaa aacatttttc cgcaacgtcc
     2041 gccgcgacga gcttcgaatg cgccatcaca tccaccggca gcacatacgc gtaagccacg
     2101 ttcatcagca tgtagatgac catcacgatg agcatcccga tgcccaagcc gagcgggatg
     2161 ttgcgttgcg gatgcgtcac ttcgccggcg atgtaagtga ttttgttcca gccgtcatac
     2221 gcccaaaacg cgccttgcat cgccgcgaca atcgccagca gcaacgcaaa accagccgga
     2281 tgaatcaccg tgcttgaggt ggtcaaattc gccgccgcgc cgcctgtcgg cagaaaaaat
     2341 gcgcccatga acagcaacgc catcgccgcc actttagcga tggtgaaaat attctgcacc
     2401 actccgccga gcttgactcc gatgtaattc accgccgtca agccgacgat aagtaccgca
     2461 gccaaacctt tcacgccgat gtccgccagc ggcgcgacat cgccgatgaa cggaaaatga
     2521 atcgtgaacg ccgacacctc cgggctgaat tccggcaatt gcaccagttt tcccgcatat
     2581 tcggcgaaca catacgcgac ggccgcgatg gaccccgttt ggatcaccgc gaaaaccgcc
     2641 catccataca ggtacgcaaa aaacgacccg aacatccgct cgaaataaac atattgcccg
     2701 cccgtttccg ggatgacgct ggcaacctcg gcattcgtga gcgcgccgaa aagcgtgagc
     2761 acacccgcca gcaaccaaac catgagcagc aatcccggtg aacccacctg ccctgccatc
     2821 aaacccggct tgcgaaaaat cccggagccg atgacgccgc cgaccaccat catgatggtg
     2881 gtgaacaaac ccagcgcagg cagcaggccg gcattgatgg aaggatggcc ggaaggtttt
     2941 tcgctcgcgc gttccgatga cagctcggat ttcaacccgc gcagcctaac cgcccgtaaa
     3001 ggcgttgttc aaggtttaag gttgctgaac tcgtgtaggg ctaaggatga atgcacgtaa
     3061 aaccatgaat ttattatttc aacaaagaat ttttcagaac caaatcgcgt aattcgtcaa
     3121 tgctcatcat gggactcctg gaatgaatca ttgcatggca attggggcag accggtcgca
     3181 agtcaataat gggatcaaca tcatattctc ggccaattcc tgacaacatc ttcaaatgat
     3241 gaacatgaat acatgcatgg ccaatttcgc catatttttc cgcataatta atcagacaca
     3301 ctgaacactt ataaccgtaa tggtcgagac acactcgctt ggcatggggg ttgcgttcat
     3361 aggcattgac cgttctttga actgttgcac cttctgcgaa ccgctgtgcg gcctcgattt
     3421 catcagcatg aaaaatatca tcaatgactc ccaaagcatt ccattgttct tttgtcgcct
     3481 tgaaatttgt cccttggaat ggaggcttta aaatcctcag atttttgaga acggaatcct
     3541 tcaacaattg tttcttgaga agaggtggat tcaagtggct tgtgtacaca gtttcaactc
     3601 gccagtccga gttttcatcc aaataaggtt tgtctgtcaa ttctcctact ccatagatgc
     3661 cgctttcctt accagcttgc caaaagataa ccttatctcc gggtttcatt agaccccgga
     3721 aatgacgaac caaccaatgg tcaatttttc ttaatcgtaa ctccttcttg agatcatact
     3781 gtttgggaat tgcttggaaa atccaaaagc acctgcttgt gacctgcttt tctttccgtg
     3841 gttcttcttc ctggtgccag ccgtggtctt ttaatctttg caatgctatc tcgcgttcag
     3901 catccgtaaa tagctttttg tattctggtt tcaaaacaag agcttcgaca gacagattca
     3961 attttccggc tgttctcagt cgctcaaagc ctttcgaaac aaccggcctt ttcaaccagt
     4021 atttagccga tgacaatcct ccatcccgac ggactttagc cagaaatcga gacggcctgt
     4081 aaccaatttg atcgcgactt ttttcgtaaa ttgacaacat cgctttgtga aatcctcgct
     4141 ctttggcgcc catgccgtaa gcatcccaac aaatgttggt cgttgtccag agattataac
     4201 gctacgttca gcacaccgaa ggcacgtgct ttcgcccttt tgtaacgaaa tttctcgaca
     4261 ccccgccctt ccatgtgtca tgctcctccc cgcgtga
//
LOCUS       DASZMM010000128         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3891051, whole genome shotgun sequence.
ACCESSION   DASZMM010000128 DASZMM010000000
VERSION     DASZMM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3891051"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..500)
                     /locus_tag="VG754_03175"
     CDS             complement(57..500)
                     /locus_tag="VG754_03175"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HWF18237.1"
                     /translation="MRNKLVVVADLGHLKAYRVEYDEVSTHPKLDLIAHIATDEADGR
                     LSDKLTDEAGRFMGGQRGHEIRASGERHNITLEFERRAVGQLAKNINHIIKRANNGEP
                     VYFAAHKEINHQILQHLDPSVRARIEKIVPEDLVKVKGMKLLEHF"
     gene            683..3685
                     /gene="ileS"
                     /locus_tag="VG754_03180"
     CDS             683..3685
                     /gene="ileS"
                     /locus_tag="VG754_03180"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWF18238.1"
                     /translation="MDYKNTLNLPQTAFPMKADLVTREPQRLAQWESMGLYQKIQAAR
                     ANGEKFVLHDGPPFANGDVHIGTALNKILKDIIVKYQTLRGKSAPYVPGWDCHGLPIE
                     FKVTQEMRKASADAPSASFDPATIRKACDAYARKYIDLQRAQFKRLGVLGDWDNPYLT
                     LNKEYEADELRLFADIVEKGFVYRGKKPVYWSIPARTALAEAEVEYHDHVSQSVFVKF
                     KIDEKHLEDYEEDPNSPTEPFPAKKNSYILIWTTTPWTLPANLAVAYSNNLTYSWIEN
                     TTDTGVEYLLVGTPLAQSIIEKAGLKDAKLLPTPYTGKNTIGQLAYHHPFCNRTGKLF
                     AGDHFVTADTGTGFVHIAPGHGLDDYNLGRQNNLPIYSPVDDDGRLAYTNDLPREQQM
                     PAEMVGKSILEKHGKSDANEAVLHELRLRKALLHQENYHHSYPYCWRSKTAIIFRAMD
                     QWFIKIDHEHFREQALDAINHVNWIPDWGKNRIESAVKSRPDWCISRQRTWGVPIPAF
                     YDAQGNAILDAQIVRNTAALFEKHGSNIWFEKSAAELWSLVKPANWTGAEPATKSNDT
                     LDVWIDSGSSSRAVLMRRPELLHSAPPTQRSALDWQADIYLEGSDQHRGWFQSSLLLS
                     LAGNNAPPFKTVLTHGFMVDADREKISKSKQAQGGYEKPQTAEAYIKKYGADVVRLWV
                     ASQDYRNDIVVSDERINKVSETYRGIRNCLRYQISNLYDFDPSKHSVPDDKLTGLDRW
                     ILAEFSKLETAVVEAYEKYEFHVVYQKISQFVAVELSAIYHDIVKDRLYTDAANSHRR
                     RSTQTALHRLVTGLCKMLAPILVFTADEAWEFVPGKTTVSVHEESWVESTFAVDAKEQ
                     KKWNSLFIFRKLVLPELEKARQNKLIGKTLEAKLNWFVGAGDIDAVLTVRSDFQEILS
                     VSQVNILPENRKLELFPARLLFNPIDRLPVDATTDPIDGRGTAIERADGQKCERCWHW
                     ETDVGSNPEHPTICARCVAAVKENLAGK"
     gene            3738..4394
                     /locus_tag="VG754_03185"
     CDS             3738..4394
                     /locus_tag="VG754_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18239.1"
                     /translation="MTERAFTLSANLATANNFLVPNYFTDVTCSMPAPKMPWPHAPAH
                     RLSQNGTFFVTASTLHKSHHFRDAKRLGVLHRGLLSVARDFGWQLEAWAVFSNHYHFV
                     AHSPQGSSNAASLSAMLKTLHVKTSAWVNKLDSSPERQVWFNFRETRLTYQRSYFARL
                     NYTHQNAVKHGLVPVANQYPWCSAVWFERTASPAQVKSIYRFQTEKLKLPDDFVPLMD
                     "
     gene            4770..>5728
                     /locus_tag="VG754_03190"
     CDS             4770..>5728
                     /locus_tag="VG754_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF18240.1"
                     /translation="MPAPKATWRNPWAWVSTLYLAEGLPYVVVMTVAVVMYKGLGISN
                     TEIALYTSWLYLPWVIKPLWSPIVDILKTRRAWIWAVQLVLGAGLAGVALTLHTSKPF
                     QYSLALLWLLAFSSATHDIAIDGFYLLATTEKEQAFFVGIRSTFYRISMVFGQGLLVI
                     LAGLIQDHTGLAKLDLRVAAKPGIAFVESVDSESLALTPVDGDLRVVTKPELIEISTA
                     PRAKRDIQALISLIKSNNIQHAFYGEEQKPAAQSTTSESWWTQNISERLAPFLRRHFG
                     PETKAKSDIAGNIGLVSLHLSKPPGREVVVTLGSRVGFGLKSD"
BASE COUNT         1336 a         1578 c         1465 g         1349 t
ORIGIN      
        1 attctgaaaa ttctgttcat tctgtcaaaa ttcagaactg ggatttcttt gggtgtttaa
       61 aaatgttcca gcagtttcat ccctttcacc ttcaccaaat cttcgggcac gattttctcg
      121 atgcgcgcgc ggacggacgg atcgaggtgc tgcaggattt gatggttgat ttccttgtgg
      181 gccgcaaaat aaaccggttc gccgttgttg gcgcgtttga tgatgtgatt gatgttcttg
      241 gccaattggc ccaccgcgcg acgctcgaat tcgagagtga tgttgtgccg ctcgcccgag
      301 gcgcggattt cgtgcccgcg ttgtcccccc atgaaacggc ctgcttcgtc tgtgagtttg
      361 tcggataatc tcccgtcggc ttcgtcagtg gcgatgtgtg ctatcaagtc gagctttgga
      421 tgggtgctaa cttcgtcgta ttccacccgg taagctttca ggtggcccag gtcagccacc
      481 accaccagct tgtttctcat acgattcaat ttaacacagg aatctgaaac gcggaaacaa
      541 aatgaaaagc tccaatgttc aaacactaaa ccgcgtaagg ttcgttttga gcttggtgct
      601 tggaatttga tggcgaccat ttccagccac attgacaccc cattctcgtg ccggtacatt
      661 acgcgttcct gaattgctga cgatggatta caagaataca ttaaatctgc cgcagaccgc
      721 ctttcccatg aaggccgatc tcgtcacccg cgagccgcaa cgcctggccc aatgggaatc
      781 catgggtttg tatcaaaaaa tccaggccgc gcgcgcgaat ggcgaaaaat ttgtgctgca
      841 cgacggcccg cctttcgcca acggcgatgt ccacatcggc accgcgctca acaaaattct
      901 taaggacatc atcgtcaagt atcaaacgct gcgcggcaaa agcgcgcctt acgttcccgg
      961 ttgggattgc cacggtttgc ccatcgaatt caaggtcacg caggaaatgc gcaaggcaag
     1021 cgcggacgcc ccgtccgcga gtttcgatcc cgcgaccatc cgcaaggctt gcgatgctta
     1081 cgcgcgcaaa tacattgatc tccagcgcgc gcaattcaag cgtctcggcg tgctcggcga
     1141 ttgggacaat ccgtatctca cgttgaacaa ggaatatgag gccgatgagt tgcgcctgtt
     1201 cgcggacatc gtcgagaagg ggttcgttta tcgcggcaaa aagccggtct attggagcat
     1261 ccccgcccgc accgcgctcg ccgaagcgga agtcgaatat catgaccacg tcagccaaag
     1321 tgtgtttgtg aagtttaaaa tcgatgaaaa acatttggag gattacgagg aagatccaaa
     1381 ctcgcccacg gaaccatttc cagcaaagaa gaactcctac attttaattt ggacgacaac
     1441 gccatggact ttacctgcaa atctggcggt agcatacagc aataatctga cctacagttg
     1501 gattgaaaat acgactgata ccggggtgga atacctgttg gtagggaccc cactcgccca
     1561 atcaattatt gagaaagccg gcctgaagga tgccaagctt ttaccgactc cctatacagg
     1621 caagaatacg attggtcaat tagcctatca tcatccgttc tgcaatcgca ccgggaaact
     1681 tttcgctggc gatcattttg tcaccgctga taccggcacg ggttttgtcc acatcgcgcc
     1741 cggccacggt ttggacgatt acaatctagg tcgtcaaaat aatctgccca tctactcgcc
     1801 cgtggatgac gacggtcgcc ttgcttacac gaacgatttg ccgcgtgaac agcaaatgcc
     1861 cgcggagatg gtcggcaaat ccattttgga aaagcatggc aagagcgatg ccaacgaagc
     1921 tgtcctgcac gaattgcgct tgcgcaaggc gctgttgcac caggaaaatt atcatcatag
     1981 ttacccttac tgctggcgca gcaagacggc gatcatcttc cgcgcgatgg accaatggtt
     2041 tatcaagatt gatcatgagc attttcgcga acaagcgctc gatgccatca atcacgtcaa
     2101 ttggattccg gattggggca agaaccggat cgaaagtgcc gttaaatcac gtcccgattg
     2161 gtgcatctcg cgccagcgca cttggggcgt gcccataccg gcgttttacg atgcgcaggg
     2221 caatgccatc ctcgacgcgc aaatcgtccg aaacaccgcc gcgctttttg aaaagcatgg
     2281 ctctaatata tggttcgaaa aatccgccgc tgaactttgg tcattggtga aacccgccaa
     2341 ctggactggc gcggaacccg ccaccaaatc caatgacacg ctggatgtct ggattgattc
     2401 cggcagttcg tcacgcgcgg ttttgatgcg ccgtccggag cttcttcatt ccgccccccc
     2461 cactcagcgc tccgcacttg attggcaagc cgatatttat ttggaaggct ccgaccaaca
     2521 tcgcggctgg tttcaatctt cgttgttgct ttcgctcgcc ggcaacaacg cgccgccatt
     2581 caagacggtc ttgacacacg gcttcatggt cgatgccgac cgcgaaaaaa tttccaagag
     2641 caaacaagct cagggcggct acgaaaaacc gcagaccgcc gaagcctaca taaaaaaata
     2701 tggcgcggat gtcgtgcgcc tgtgggtcgc gtcgcaggat tatcgcaacg acatcgtcgt
     2761 gagcgacgag cgcatcaaca aagtcagcga gacctaccgc ggcatccgca actgcctgcg
     2821 ctaccagatc tcgaatcttt acgatttcga tccttcgaaa cattccgtgc cggacgacaa
     2881 actcaccggc ttggaccgct ggatactcgc tgagttttca aaattggaaa ccgccgtcgt
     2941 cgaggcatac gagaaatacg agttccacgt cgtttatcaa aaaatcagcc agttcgttgc
     3001 cgttgaattg tccgccatct atcacgacat cgtcaaagac cgtctttata ccgacgctgc
     3061 aaattcacat cgccgccgct cgacccaaac cgcgttgcat cggttggtga ccggcctatg
     3121 caaaatgctc gcgccgatac tggtgtttac ggctgatgag gcatgggagt ttgtgccggg
     3181 gaaaacaact gtctctgtcc atgaagaaag ctgggtggag tccacttttg cagttgatgc
     3241 taaagaacaa aaaaaatgga attcactttt catttttcga aaactagttt tgccagaatt
     3301 agaaaaggcc cgtcaaaaca agctgatagg caaaactctt gaagcgaagc tgaattggtt
     3361 tgttggggct ggagatattg atgctgtttt gacagttcgg tctgatttcc aagaaatcct
     3421 gtcagtgtct caagtaaata ttttgccaga gaataggaaa cttgaattgt ttccggcgcg
     3481 tctgctcttc aatccaattg atagattgcc cgttgatgca actactgatc ccatcgacgg
     3541 acgcggaaca gctatcgaac gcgccgacgg ccaaaagtgc gaacgttgct ggcattggga
     3601 aacggatgtc ggttccaatc ccgagcatcc caccatctgc gcccgctgcg tcgccgcggt
     3661 gaaggaaaat ttggctggca agtaaaggcg cgctcaaatt tccgccaggt cttggactgc
     3721 ggcagtcccc tgccgctttg accgagcgcg cgttcacgct ttccgccaat cttgcaaccg
     3781 cgaacaattt ccttgtccca aattatttca cggacgtaac ctgctccatg cctgcgccaa
     3841 aaatgccctg gcctcacgct cctgctcaca gactttctca gaacggaact tttttcgtca
     3901 cagcaagcac gctccacaag agtcaccatt ttcgcgacgc caaacggctg ggcgttttgc
     3961 atcgcggatt gctttcagtc gcccgggatt ttggctggca attggaagca tgggcggtgt
     4021 tttcgaatca ttaccatttt gtcgctcatt ctccacaagg ttcgtcgaac gccgccagcc
     4081 tttctgcaat gttaaaaaca ttgcatgtga aaacgagcgc gtgggtgaac aagttggact
     4141 cgtcgcccga acgtcaggtt tggttcaact tccgagaaac gcgtttgaca taccaacgtt
     4201 cctactttgc gcggctcaac tacacacatc aaaatgcggt gaaacacgga cttgtgccgg
     4261 ttgccaacca atatccatgg tgttccgcag tgtggttcga gcgaacggcg tccccggccc
     4321 aagtcaaaag catttaccgc tttcaaacgg agaaattgaa gttacctgat gattttgtgc
     4381 cgctgatgga ttgatgccaa ttggtcttcc gaagaaaagc ggcagaggac tgcaactcca
     4441 agacgctctc gcgccgtcca agcccgctca aattccgtca acttttccac tacagcactc
     4501 ttggtatttt taaccaagga aaagtttcgt ggcaggcata aatttgcatg ccggtcaaag
     4561 cggcagagga ctgccgcact ccaagacgct ctcgcgccgt ccaagcccgc tcgaatttcc
     4621 gccaggtctt ggactgcggc agtcccctgc cgctttgacc cagagcgctt gctttcatca
     4681 aagtgaatct ttgtctgcga cctgtttcct ccttgccgca tcttcatctt tgctgctatg
     4741 aaacgcgcgt cttgagcgac tgaaaaccga tgcccgcacc aaaagcaacc tggcgaaatc
     4801 cgtgggcgtg ggtttccact ttgtatcttg ccgaggggct tccttacgtg gtggtgatga
     4861 cggtggcggt cgtcatgtac aaagggctcg gcatctccaa cactgagatc gccctctaca
     4921 caagctggct ctatttgccg tgggtcatca agccgctttg gagccccatc gtggacattc
     4981 tcaagacgcg gcgcgcgtgg atttgggcgg tgcaactcgt tctcggcgcg ggactggccg
     5041 gcgtggcgtt gacgcttcac acttcgaagc cgttccaata ttcgctggcg cttttgtggc
     5101 tgctggcatt cagctcggcg acgcatgaca tcgcgattga cgggttttat ctgcttgcaa
     5161 cgacggaaaa ggagcaggcg ttttttgtcg gcatccgcag cacgttctat cgcatctcga
     5221 tggtgttcgg ccaggggttg ctggtgatcc ttgcgggact tatacaggac cacaccggat
     5281 tggcgaaact ggatttgcgc gtcgccgcaa aaccgggcat cgcgttcgtt gaatcggtcg
     5341 attctgaatc gcttgcgctc acgccggttg acggcgattt gcgcgtggtg acgaaaccgg
     5401 agttgattga aatcagcacc gcaccacgcg cgaaacggga catccaggcg ctcatctcgc
     5461 tgatcaaaag caacaacatc cagcacgcat tttacggtga agaacaaaaa cctgccgcgc
     5521 agagcacaac ttccgaatcg tggtggacac aaaatatttc cgaacgatta gcgcccttct
     5581 tgcgccggca tttcgggcct gaaaccaagg cgaaaagcga catcgctgga aatatcgggc
     5641 tcgtttcgct gcatctttcc aaaccgcccg ggcgcgaagt ggtggtgacg ctcgggtcgc
     5701 gcgtcggctt cggcctcaaa tcggatga
//
LOCUS       DASZMM010000129         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_598712, whole genome shotgun sequence.
ACCESSION   DASZMM010000129 DASZMM010000000
VERSION     DASZMM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598712"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..866
                     /locus_tag="VG754_03195"
     CDS             111..866
                     /locus_tag="VG754_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HWF18241.1"
                     /translation="MPTITISVQRSAAAPDESPAFESHQVSVGEKASVLDALFALQRG
                     PCPDLAFRFSCRVGMCGSCAMVVNGREQLTCSTLVKTIGDTLRVQPLRNLPVTRDLAV
                     DMKPFFAAYQRVMPQFIPRDDLDKGDFYPIPHDSKEGRALNHQPQCIDCGACYSACTL
                     VTLHPRYAGPMALNRALNLVVDPRDKARAERLELVGGEAGVFRCHTLGNCRDVCPRGI
                     SPTHSIERLKRLAVFGMFRKMFSKWKANSTPTS"
     gene            836..2539
                     /locus_tag="VG754_03200"
     CDS             836..2539
                     /locus_tag="VG754_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018924896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWF18242.1"
                     /translation="MESQLDTDILILGAGGAGLMAALHAFWRDPSLDITLVSKGMLGK
                     SGCTRMVQGGYNVALDPKDSTLAHFEDTVKGGAFLNDQELAWTLVDDAPRIVRELENK
                     IGCLFDRRPDGRIHQKAFAGQSFDRTVHVGDLTGIEIMARLRDQIFATSIRCLEYTRG
                     LELLTAGDSKEIIGAVLLDIQSGEFIVVRAKAVLLCTGAGPLMYQRAACAQEKAMDGV
                     AMAYRAGAHLMDMEMVQFHPTGLVVPGSRLNGALLEEGLRGAGARLFNGQGERFMTRY
                     DPERAERSTRDRVSRGSYMEMTADRGTQNGGVWIDVSHLGAEFVEKNFTGMRDRCLRI
                     GFDLAREKVEVAPTAHFNMGGVRINRDGFTNLEGLFAAGEDSSGVHGANRLGGNGVAE
                     STVFGARVGDAAVDWIKQRKHAEPRQDQIAQARASAEQFLERKNGEDAWVLREELGKV
                     MWEHAGIVRSGAKLRLALEEIATLSQRAAQISVPGGRAFNLTWQQALDMRNLLAASDL
                     IARSALMREDSRGAHYREDFPNTDQANWLKNIYIARNGEGAKLWTEPVKLTRLKPPDL
                     L"
     gene            2575..>3353
                     /locus_tag="VG754_03205"
     CDS             2575..>3353
                     /locus_tag="VG754_03205"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWF18243.1"
                     /translation="MPDKFSNKPIRVAITGAAGAIGYSLLFRIAAGEMFGPEQPVILH
                     LVDIEPALPALQGVMMELDDCAFPLLKGMVATANLDDGFRGVNWALLVGSVSRKPGLE
                     RKDLLNINGKIFVGQAQAIQKTAAPDIRVLVVGNPCNTNCLIGMNNAPEVPRDRWHAM
                     TRLDENRARFQLARKAEADITEVTNMAIWGNHSATQYPDFYNAKIKGRLVPEVITDET
                     WLQNEFVTTVAQRGTSVLKARGAGSAASAANAIIDSVHSIV"
BASE COUNT          710 a          966 c          999 g          678 t
ORIGIN      
        1 ggggggtgag ggtcgtatag gaggtaaagc tggaagtaac tcgattcata actaggagtg
       61 gaaattttca aaacaattgc aacttccttt cggcccatta cgattgcctt gtgccaacca
      121 tcacgatcag cgtgcaacgc agcgccgccg cgccggatga aagtccagcc ttcgaatcgc
      181 accaggtttc cgtcggtgag aaggcaagcg tgctcgatgc cctctttgcg ttgcagcgcg
      241 ggccgtgtcc cgacctggcg tttcgctttt cctgccgggt cggcatgtgc ggctcgtgcg
      301 ccatggtggt gaatggccgc gaacaattga cctgcagcac gctggttaaa accattggcg
      361 acacactgcg cgtccaaccg cttcgcaacc ttcccgtcac gcgcgatctt gccgtggaca
      421 tgaaaccatt ttttgccgcc taccaaaggg tgatgccgca attcatcccc cgcgacgatt
      481 tggacaaggg cgatttttat cccatcccgc acgacagcaa ggaaggccgc gcgctgaatc
      541 atcaaccgca atgcattgat tgcggcgcgt gttactcggc ctgcacgctg gtgacgctgc
      601 atccccgtta tgccggcccg atggcgttga accgcgcgtt gaatctcgtc gtggatcctc
      661 gcgacaaggc gcgcgccgaa aggctcgaac tcgtcggcgg tgaagcgggt gtttttcgat
      721 gtcacacatt gggcaactgc cgcgatgtct gcccgcgcgg catttcgccg acgcattcga
      781 ttgaaagatt gaaacggctc gctgtattcg ggatgtttcg caagatgttc tcaaaatgga
      841 aagccaactc gacaccgaca tcttgattct cggcgcgggc ggggctgggt tgatggcggc
      901 ccttcatgcg ttctggcgcg acccttcgct ggacatcacg cttgtttcga aaggcatgct
      961 cggcaagagc ggctgcaccc gcatggtcca aggcggctat aacgtcgcgc tcgacccgaa
     1021 ggattcaacg ctggcgcatt tcgaggacac cgtcaaaggc ggcgcttttc tcaatgacca
     1081 ggaacttgcg tggacgctgg ttgacgacgc gccgcgcatc gtgcgcgaac tggaaaacaa
     1141 aatcggctgt ctctttgatc gccgtccgga cggacgcatt catcaaaaag cttttgccgg
     1201 tcaaagtttt gatcgcacgg tgcacgtggg tgatttgacc gggatcgaaa tcatggcgcg
     1261 tttgcgcgac cagatttttg cgacgagcat ccgttgcctc gaatacacgc gcggattgga
     1321 actgctgacc gccggtgata gcaaagaaat tatcggcgcg gtgttgctcg atattcaaag
     1381 cggcgaattc atcgtggtgc gcgccaaggc ggtgttgctt tgcaccggcg caggcccgct
     1441 gatgtatcaa cgcgccgcct gcgcccagga aaaagcgatg gacggcgtgg cgatggccta
     1501 tcgcgccggc gcgcatctca tggacatgga gatggtgcaa tttcatccga cgggtttggt
     1561 cgtgccgggc tcgcggttga atggcgcgtt gctggaagaa ggtttgcgcg gcgcgggcgc
     1621 gcggttgttc aacggccagg gcgaacgttt catgacgcgt tacgatcccg aacgcgcgga
     1681 gcgttccacg cgcgaccgtg tttcgcgcgg cagttacatg gaaatgacgg cggatcgcgg
     1741 cacgcagaat ggcggcgtct ggattgatgt ctcgcacctt ggcgcggaat tcgtggaaaa
     1801 aaactttacc gggatgcgcg accgatgcct tcgcattggc ttcgaccttg cgcgcgaaaa
     1861 agtcgaagtc gcacccacag cgcatttcaa catgggcggc gtgcgcatca atcgcgacgg
     1921 attcacaaat ctcgaagggc tcttcgcagc cggtgaggat tccagcggcg tgcatggcgc
     1981 aaaccgcctt ggcggcaatg gcgtcgcgga atcaacggtg ttcggcgcgc gcgtcggcga
     2041 tgccgcagtg gattggatca agcaacgcaa acacgcggag ccgcgtcagg atcaaatcgc
     2101 gcaagccagg gcatcagcgg agcaatttct cgagcgaaaa aatggcgagg atgcctgggt
     2161 gttgcgcgaa gaactcggca aagtgatgtg ggaacacgcg ggcattgtgc gcagcggcgc
     2221 gaaattgcgg cttgcgctgg aagaaatcgc aacgctttcg caacgcgctg cgcaaatttc
     2281 cgttcccggc gggcgcgcat ttaatctcac ctggcagcag gcgctcgaca tgcgcaattt
     2341 gctcgcggct tccgatttga ttgcccgctc cgctctgatg cgcgaagaca gccgcggcgc
     2401 gcattaccgc gaagattttc ccaataccga tcaggccaac tggctcaaaa atatttacat
     2461 cgcccgcaac ggcgagggcg caaagctctg gacggagcct gtgaagctca cgcgcctcaa
     2521 accgcctgat ttattgtgaa agcaccaagc aaaacccgtt aaccacatct catcatgcct
     2581 gacaaatttt ctaacaaacc catccgcgtc gccatcaccg gcgctgccgg agcgattggt
     2641 tattcgcttt tgttccgcat cgccgccggc gaaatgttcg ggccggaaca accggtcatt
     2701 cttcacctgg ttgacatcga gcctgccctg cccgcgttgc aaggcgtgat gatggaactc
     2761 gacgattgcg cgtttccgct cctcaaggga atggtcgcca ccgcaaatct ggatgacggg
     2821 tttcgcggcg tgaattgggc gctgctcgtg ggcagtgttt cgcgcaaacc cggtctcgaa
     2881 cgcaaggatt tgctgaacat caacggtaaa attttcgtcg gtcaggcgca ggccatccaa
     2941 aaaaccgcag caccggacat tcgcgtgctg gtcgtcggca atccctgcaa cacaaactgc
     3001 ctcatcggca tgaataacgc gccggaagtg ccccgcgacc gttggcatgc catgacgcgc
     3061 ctcgatgaaa atcgcgccag gttccagctt gcccgcaagg cggaagcgga catcaccgag
     3121 gtcaccaaca tggcgatttg gggcaatcat tcggcgacgc aatacccgga tttttacaat
     3181 gccaaaatca aaggccgcct cgtcccggaa gtcatcacag atgaaacatg gctgcaaaat
     3241 gaattcgtga ccacagttgc gcaacgcgga acatcggtct tgaaagcgcg tggagcgggc
     3301 agcgccgcca gcgcggccaa tgccatcatt gacagcgtgc attccatcgt gca
//
LOCUS       DASZMM010000130         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_862624, whole genome shotgun sequence.
ACCESSION   DASZMM010000130 DASZMM010000000
VERSION     DASZMM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862624"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VG754_03210"
     CDS             complement(<1..678)
                     /locus_tag="VG754_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF18244.1"
                     /translation="MTVQNEKTTTSRRRLIALGCAGTAAGLWLYYSNQYVPRILRELV
                     ADSGRAILQPRHTPTPMLWDDNAITAAWLGHSTVLLNFYGLTILTDPVLFRRVGADLG
                     IGTVGPKRLVASALTMRQLPHIDLVLLSHAHMDHFDLATIRRLPASTKAVTANKTADL
                     FADTRIKQPRELAWGDSTTVRTRKGDISIRAFEVAHWGARWRHDTFRGYNGYTLEREG
                     KKIIFGGD"
     gene            complement(743..1405)
                     /gene="aat"
                     /locus_tag="VG754_03215"
     CDS             complement(743..1405)
                     /gene="aat"
                     /locus_tag="VG754_03215"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWF18245.1"
                     /translation="MTRNAQSPFPDPRLADDKGLVAIGGDLSEKRLLAAYRSGIFPWT
                     VNPVTWWSPNPRAIFELEQIHVPKSLEKIIRKGMFEITFDRAFRQVIEGCAAPARGRR
                     STWITPEFIEAYTNLFKAGHAHSVECWSGKELAGGIYGVALGGFFAGESMFHRVSNAS
                     KVALIHLLRHLRERGFSLFDIQMLTPATRQLGAIEISRLEYLRHLSKALEKRPNVINR
                     MN"
     gene            complement(1414..1881)
                     /locus_tag="VG754_03220"
     CDS             complement(1414..1881)
                     /locus_tag="VG754_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18246.1"
                     /translation="MKLIKADGEKFSFLIERRERQMLFRVLELYPLVPASHHKISKSA
                     HQSEDQRLLEEALTQERTKNKKQVEEMLKTKSRFLEHEHGYEFCLKTEQMEWLLQVLN
                     DVRVGSWLLLGSPDGPAETLAKLSHETASHFWSMEVAGQFQMVLINAMNRRIP"
     gene            complement(1882..2187)
                     /gene="clpS"
                     /locus_tag="VG754_03225"
     CDS             complement(1882..2187)
                     /gene="clpS"
                     /locus_tag="VG754_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWF18247.1"
                     /translation="MAETATPEILPDIERQEETKETTELEPGYLVICWNDPVNLMDYV
                     THVFQTVFGWKKGKAEHHMLQVHHQGKSVLIRESMEKAEHYVHLLQQYSLHATMERD"
     gene            complement(2368..3342)
                     /locus_tag="VG754_03230"
     CDS             complement(2368..3342)
                     /locus_tag="VG754_03230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWF18248.1"
                     /translation="MKRKALFFLSIGLLVGVASTPAQPGNQPANPSANPAPVPATNQA
                     PKTDFSKIFKSDKEKVSYAIGMSWGSGLKGRLKKEEVDIDMDTFAKGFMDNLGVGPSL
                     ITEAQEKEILTEFGAEMRGKQQAKQKQMAEENRIKGEKNKADGDAFLAKNKTEPGVVT
                     LPDGLQYIVEVEGKGESPKADSEVSVNYRGTLIDGTEFDSSFKRNQPFTTRVQGGIIK
                     GWTEALELMKPGAKWKLFIPPSLAYGERPMGPTIAPNSTLIFEIELLSVKQGSPLTPT
                     PSSTVRPPNAPLTSDIIKVPSAEEMKKGAKIETIKPEDLDKEKAKTNQ"
     gene            complement(3385..>3584)
                     /locus_tag="VG754_03235"
     CDS             complement(3385..>3584)
                     /locus_tag="VG754_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18249.1"
                     /translation="QRWLFDVFECAVWPRLDQKSWITSFLSNNESRLVKPLLRHVNAH
                     GSACFLIRWDNHSTKKDKKIG"
BASE COUNT          780 a         1002 c          922 g          880 t
ORIGIN      
        1 atcgccgccg aagatgattt ttttgccttc gcgttccaag gtgtaaccat tgtaaccgcg
       61 gaatgtgtca tgccgccaac gcgcgcccca atgggcgact tcaaatgcgc ggattgaaat
      121 gtcgcccttg cgcgtgcgca cggtggtgct gtcgccccag gcaagttctc tcggttgctt
      181 gatgcgcgta tctgcgaaca agtcggcggt tttgttggcg gtgacggctt tcgtcgaagc
      241 cggcaaacgc cggatggtcg caaggtcgaa atgatccatg tgcgcgtgtg aaagcagcac
      301 caaatcaatg tgcggcaact ggcgcatcgt caatgccgag gcgacaaggc gtttcgggcc
      361 gacggtgcca atgcccaaat ccgcgcccac gcgccggaac aaaaccgggt cggtcaaaat
      421 cgtgaggccg taaaaattca gcaaaacggt ggaatgtccc agccatgcgg cagtgatcgc
      481 gttatcgtcc caaagcatgg gagtgggcgt gtggcgcggt tggaggatgg cccggccgga
      541 atcggcgacc aattcgcgca gaattcgtgg gacatattga ttggaataat aaagccagag
      601 gcctgccgcg gttccagcgc aacccagcgc aatcaagcgt cgtcttgagg tcgttgtctt
      661 ttcgttctgc acagtcattt cgggaattag accatcaaac actgcaaagg ttcgaaatga
      721 attccaaaga taagctccaa ttctaattca ttctattgat cacatttggc cttttttcaa
      781 gggctttgga caaatgccga agatattcca agcgggaaat ttcaatcgca cccaactgcc
      841 gggttgcagg cgtgagcatc tggatgtcga acaggctgaa tccgcgctcg cgcaaatgcc
      901 ggagcagatg aatcaacgcc actttggaag cgttgctgac gcggtggaac atggattcac
      961 cggcgaaaaa accgccaagc gccacgccat aaatccctcc agccagttct ttgccgctcc
     1021 agcattccac gctgtgcgca tggccggctt tgaagagatt cgtgtaggct tcaatgaact
     1081 ccggcgtgat ccatgtgctg cggcgtcccc gggcaggtgc cgcgcaacct tcaatgacct
     1141 gccggaacgc ccgatcaaat gtgatttcaa acattccctt gcggatgatt ttctccaaac
     1201 ttttcggcac atgaatctgc tccaactcaa aaatcgcgcg cggatttggc gaccaccatg
     1261 tcaccggatt gaccgtccat ggaaaaatcc cgctgcgata agccgccagc aaccgctttt
     1321 ccgacaaatc gccgccgatg gccaccaacc ctttgtcatc cgccagacgc gggtcgggaa
     1381 atggactttg ggcattccga gtcatcacgc aagctatgga atccgccgat tcatcgcatt
     1441 gatgaggacc atctggaact ggcccgccac ctccatggac caaaaatgcg aggcggtttc
     1501 atggctcaat tttgcgagtg tttccgccgg tccatccggc gaaccaagca acagccagct
     1561 tcccacccgg acatcgttga gcacttgcag cagccattcc atctgttcag ttttcaagca
     1621 aaattcataa ccatgctcgt gctcaagaaa tcgagacttt gtcttgagca tctcctcgac
     1681 ctgtttcttg ttctttgtgc gttcctgtgt cagcgcctcc tccaacaaac gctggtcctc
     1741 ggattgatgc gcgcttttgc tgatcttgtg gtgtgaggca ggaaccaacg gatacaactc
     1801 cagaacgcga aacagcatct gacgctcgcg gcgttcgatg aggaagctga acttttcgcc
     1861 gtccgccttg atgagtttca cctagtctcg ctccatggtt gcgtgcaggc tgtattgttg
     1921 cagaagatgg acgtaatgct cggctttttc catgctctcg cgtatcagca cgctctttcc
     1981 ctgatgatga acctgcaaca tgtgatgttc cgcctttcct tttttccagc cgaagacggt
     2041 ctggaaaacg tgcgtcacat aatccatcaa attgaccgga tcattccaac aaatgacaag
     2101 ataacccggc tccagttccg tggtttcttt cgtctcctct tgccgttcga tgtccggcag
     2161 gatttccggt gtagccgtct cagccatggt tgcaaaatag aagaggactg cgcgattgtc
     2221 gagcggtcag aaaaagcgcc aagcgtccaa gcaccaacct ccatggaatc atcaagcacc
     2281 taaccaaaaa acgctaattg cacatcggct tggtgcttgg aatttcgaac ttctctgggg
     2341 cttggatgtt gaagcttgga gcttcaatta ttggttggtc ttcgcctttt ccttgtccaa
     2401 atcttccggc ttgatggttt cgatcttggc gccttttttc atttcctcgg cggaaggcac
     2461 cttgatgatg tcgcttgtca acggcgcatt gggcggacgg accgtcgagc tcggagtcgg
     2521 cgtcaacggc gagccttgct tgactgacaa caattcgatt tcaaagatca acgtcgaatt
     2581 cggcgcgatg gtcggcccca ttgggcgctc gccataggcc aaactcggcg gaatgaacag
     2641 cttccacttc gcgccgggct tcatgagttc caacgcctcc gtccagccct tgatgatgcc
     2701 cccttgaacg cgcgtcgtga acggctggtt gcgcttgaac gaactgtcga actcggtgcc
     2761 gtcaatcaac gtgccgcgat aattcacact cacctcgcta tccgccttgg gactttcgcc
     2821 tttgccctcg acttcaacga tatattgcag accgtcagga agcgtcacga cgcccggctc
     2881 ggtcttgttt ttggcaagga acgcatcgcc gtcggctttg ttcttttcgc ccttgatgcg
     2941 gttttcctcg gccatttgtt tctgcttggc ctgctgcttg ccccgcattt ccgcgccgaa
     3001 ctccgttaaa atttcctttt cctgcgcctc ggtgatgagt gacggcccga cgccgagatt
     3061 gtccatgaaa cccttggcga aagtgtccat gtcaatatcg acctcctcct tcttgaggcg
     3121 gcctttcaaa cccgagcccc agctcatgcc gatggcgtaa ctgacctttt ccttgtcgct
     3181 cttgaatatc ttgctgaaat cggtcttggg agcctggttc gtcgccggga ccggagcagg
     3241 attggcggac gggttggcgg gttggtttcc aggttgggcc ggggtgctcg ccacgccgac
     3301 gagcaagccg atgcttagaa aaaatagcgc tttacgtttc ataaaatgtt agagcggata
     3361 gaatctttca aaaacgggca actttcaacc gatttttttg tcttttttgg ttgaatgatt
     3421 atcccatctg atgagaaagc aagcgcttcc gtgtgcgttg acatgacgca ataatggctt
     3481 gaccagcctt gactcattat tgcttaaaaa cgaagtaatc cacgactttt ggtcaagcct
     3541 tggccaaacc gcgcactcaa aaacatcgaa cagccaacgc tgaa
//
LOCUS       DASZMM010000131         7163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_42483, whole genome shotgun sequence.
ACCESSION   DASZMM010000131 DASZMM010000000
VERSION     DASZMM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42483"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /gene="murF"
                     /locus_tag="VG754_03240"
     CDS             complement(<1..1029)
                     /gene="murF"
                     /locus_tag="VG754_03240"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWF18250.1"
                     /translation="MEKRSLKFIAEACGGELANGSPQAQVTRICTDSRQAQAGDLFVA
                     LAGERFDGHDFLSEVAQNGVTALLVERAKTSGLLHSAIRNPKSPINKCGVITVDNSRQ
                     ALGRLAARYREDFNLPVVAIGGSNGKTTTKELVASVLRQKFKTLWSEASFNNDIGVPL
                     TLLNLESSHEAAVLEVGTNHPGELAPLVRMVQPRFGVLTSIGREHLEFFGDLAGVAME
                     EGWLAELLPAGGTLFLNGDSEWTGEIARRGRAKVWRIGLAIENDWRPGNVQMDEQGMR
                     FWVAGSKPNIAGEYRINLLGRHQVVNAVFAIAIGAELGLTREEIQRGLNECKPAKMRL
                     QRWNWNGVC"
     gene            complement(1080..2996)
                     /locus_tag="VG754_03245"
     CDS             complement(1080..2996)
                     /locus_tag="VG754_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWF18251.1"
                     /translation="MAKKLQFKRLFTLTFLLALAFAGLGYRLVDLQVVRHGELSKEAR
                     SNTEFTRRLEPRRGDILDCKGNLLATSVFVKTVCADPSLIGNYSADVARVLAPLLQMN
                     ESDLARRLVPRARRNEKGQMVPVRYVVLKRKVSTDAWEKIQDAMNKLSFGVDEQKFSK
                     KDQAFFRNLRNGGIMTDPVDGQLRIYPNGSLAAHVIGFVGVTEQTNGPLTEITSGMGG
                     IERWYDSKLKGTRGWLVSEHDRQQREMVSLREQDIAPHDGLTVVLTIDSAIQNFLESA
                     LEEGLKKHTPKSISGIVMRPRTGEILAMASLPSFDANNPGAPGEAVDALNNRVISDVA
                     EPGSTFKIVVVSGALNEGVVRLTDTFDCENGMFWYAGKRLTDHAHYGVLSVSNIITKS
                     SNIGAAKIGLKMGETRLHDYVTAFGFGRKTGIPLPWESRGLVSEVSNWTKLSITRIPM
                     GHEVGATCLQTAMAMSAVANKGVLMQPLLVDRLVDTDGSTAIKYYPQAVRRVISEEAD
                     KDMVEALKSVVTQDGTAVKAAMEHYTVAGKTGTAQKAENGYYGDKFFSSFVGFFPADN
                     PEILIYVALDSPNSGHYGGDVAAPIFREIAEKAANYLNIRPDKDDPGLPGAPTLTPIA
                     TPDQPIKTAEARVP"
     gene            complement(3077..3472)
                     /locus_tag="VG754_03250"
     CDS             complement(3077..3472)
                     /locus_tag="VG754_03250"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWF18252.1"
                     /translation="MARNRKNQSAGLLVGPALKASLICFFILICCVGYVWQKKQIAEL
                     SHHIRNQENRLADLRYTNDQLKKQIAMLLSPPKLEARARELKLGLVPPQPAQIWRLPE
                     PAAEIPAASKPEREYAAGRTGAVAAVMEQ"
     gene            complement(3474..4403)
                     /gene="rsmH"
                     /locus_tag="VG754_03255"
     CDS             complement(3474..4403)
                     /gene="rsmH"
                     /locus_tag="VG754_03255"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWF18253.1"
                     /translation="MPNYSHNPVMVAEVLAALKPRPDGRYADGTAGGGGHAAAILAAS
                     SPTGWLCGCDRDGAAVEAAQARLAEFAGRFEIRRGNFAEMAQMWEPGSFDGVLLDLGV
                     SSPQLDRAERGFSFQQEGPLDMRMDTRQGTTAADLVNRLSADELAKIFWELGDEPQSR
                     RFARAIEMDRQQRPFTTTGQLAGLIERLSPRRGQRTHPATKVFQALRMAVNDELGSLR
                     RGLDGAVKLLKPGGRLAVITFHSLEVRVVKEFGRERSRDYEFEGPVDVPELRRPRKPE
                     LRWIERKAVKPGAAELAENPRSRSAQLRTMEKI"
     gene            complement(4416..5288)
                     /locus_tag="VG754_03260"
     CDS             complement(4416..5288)
                     /locus_tag="VG754_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18254.1"
                     /translation="MSFSRLLAAGKSLVGMTNSQSPYRMRAENLLPKFESTKNPFASI
                     PKTEAPKSEPATPPPIAAPCAVMVAPMETYPLFDVELKPILAVAAVAAKMNETEAVAI
                     PVNAPAKVSPAKASVQPVLEQRRVMQPEPNRGLVDASPLGLKTGTPTRAPLPAPVIAP
                     PKVPANQPAQPMPTEPPTPSPDHESSRIAAKPAKSEQTFGWLAPLFKLFRFGRRSQNI
                     NARKPVQTELSLDRVKVIRNDLNETDLEVVTIKKSEKADAPPVRLAPLAEADAGNFSR
                     IASRIFGTSQSLVR"
     gene            complement(5295..5756)
                     /locus_tag="VG754_03265"
     CDS             complement(5295..5756)
                     /locus_tag="VG754_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18255.1"
                     /translation="MPSTDNETIHYTSLFRHGVDEKRRVQIPAKWRPSEPDVEFTLIL
                     WPNGGQPDANLLVLPPTEMKALAEKIRTMSSADPKASALRRLIGSKSASVALDKAGRI
                     CIPDTMAKAVGIENDAVLVGLVDRFEIWNPERYETVSAVDAALLPEAFKLI"
     gene            6156..>7163
                     /gene="queA"
                     /locus_tag="VG754_03270"
     CDS             6156..>7163
                     /gene="queA"
                     /locus_tag="VG754_03270"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWF18256.1"
                     /translation="MLTNDFDFQLPPELIAQQPAMRRDQSRLLVLQRGSGKLTHRTFP
                     DLLEYLNPGDVLVLNNSRVIPARLRGVNTKSRGQFEILLLEENATNDWWVMLRPGKRA
                     RVGTEIIFRDLAGNKSAVHARVIETNAEGHRRLQFSGVADIRDVLDALGEIPLPPYIA
                     RANADELAADKIRYQTVYAASAGSVAAPTAGLHFTEELLSAIRAKGVHVSFVTLHVGL
                     GTFAPVKAETLSEHVMHEERYEVSDETARLVNDAKNSDKRVIAVGTTSVRVLESVAQK
                     NQGRIVAGNGRTRIFIFPPAEFQIVDALLTNFHLPRSTLLMLVSAFAAPRELRGREMI
                     LQ"
BASE COUNT         1488 a         2217 c         1831 g         1627 t
ORIGIN      
        1 gcatactccg ttccaattcc agcgttggag gcgcattttt gcaggtttgc attcatttaa
       61 tccccgttgg atctcctccc gggtcaaacc tagctcggcc ccaatcgcaa tggcgaagac
      121 cgcatttaca acctgatgac gcccaaggag atttatacgg tattcccccg caatattcgg
      181 ttttgaaccg gccacccaga accgcatccc ctgctcgtcc atctggacat tgcccggacg
      241 ccagtcgttt tcgatggcga ggccgattcg ccacaccttt gcacgaccgc gtcgggctat
      301 ttccccggtc cattcgctgt ccccgttcag gaaaagcgtc ccgccagccg gcaaaagctc
      361 cgccagccag ccttcctcca tggcgactcc tgccaaatct ccaaaaaatt ccaaatgttc
      421 gcgcccaatg ctggtcagaa cgccaaatcg cggttgaacc atgcgaacca agggggccag
      481 ttcgcccgga tggttcgtcc ccacctccaa tacggcggct tcgtgggacg attccagatt
      541 caacaaggtc aagggcacgc cgatgtcatt attgaagctc gcctcgctcc agagagtctt
      601 gaatttttga cgaagcaccg atgccaccag ctccttggtg gtcgttttgc cattggaacc
      661 gcctatcgca acgaccggca aattgaaatc ttcccgatac cgcgcggcca atctgcccaa
      721 agcttgacgc gaattgtcca cagtaatgac accgcattta ttgattggag acttgggatt
      781 gcggattgca gaatgcaaga gaccggatgt tttcgcccgt tccaccaaca atgccgtgac
      841 gccattttgc gcgacttcag acaggaaatc gtgcccgtca aatcgctcgc ccgccagcgc
      901 cacgaacaga tcgcccgcct gcgcctggcg cgaatcggtg cagatacggg tcacctgcgc
      961 ctgcggcgaa ccgtttgcca gttctccgcc gcaagcctcg gcaataaatt ttaaactgcg
     1021 cttttccatc aaatattatt caggcaattc tttcgcgtga gtgcaagagc aatcgaactt
     1081 catggaaccc tcgcctccgc ggttttgatc ggttggtcgg gcgtggcgat cggtgtcagt
     1141 gttggcgcgc caggcaaacc cgggtcatcc ttgtccggcc ggatgttcag ataattggcg
     1201 gccttctcgg cgatttccct gaaaatcggc gctgcaacgt ctcccccata atgaccgctg
     1261 ttgggagaat ccagcgccac gtaaatcaaa atctcaggat tgtccgcagg gaaaaatccg
     1321 acgaacgacg aaaagaattt gtcgccgtaa taaccgttct cggctttttg ggccgtgcct
     1381 gttttgccgg caacggtata atgttccatc gccgctttca cagcggttcc atcttgcgtc
     1441 accacgcttt tcaacgcctc gaccatgtct ttgtccgcct cctcgctgat gacccggcga
     1501 acagcctgcg gataatattt gatcgcagtt gaaccatccg tatccacgag gcggtccacg
     1561 agcaatggct gcatcaacac gcccttgttc gccaccgcgc tcatcgccat ggccgtctgc
     1621 aaacaagtcg cgcccacttc atggcccatt gggatgcgag tgatggaaag cttcgtccag
     1681 ttgctcactt cagacacaag cccgcgcgat tcccatggca gcggaatgcc cgtctttcga
     1741 ccgaaaccaa atgccgtcac ataatcatgc aaacgcgttt cgcccatctt gaggccgatt
     1801 ttcgccgcgc cgatgttgga tgatttcgtg atgatgttgc tgaccgaaag cactccgtaa
     1861 tgagcgtggt ccgtcaatcg ctttcccgca taccaaaaca ttccgttctc gcaatcaaac
     1921 gtgtcagtca gcctcacaac gccttcattc agcgcgccgg agacgaccac gatcttgaaa
     1981 gtcgaaccag gctcggcgac atcgctgatg acgcggttgt tcagggcatc aacagcctcg
     2041 ccgggcgcgc cgggattgtt cgcgtcaaaa ctcgggagcg aagccatcgc caggatttct
     2101 cccgtgcgcg gccgcatgac gattccggag atgctctttg gcgtgtgttt tttcagccct
     2161 tcctcgagcg cgctttcaag aaaattttgg atggccgaat caatcgtcag caccacggtc
     2221 aagccgtcgt gcggcgcgat gtcctgttca cgcagcgaaa ccatttcgcg ttgttgacgg
     2281 tcgtgttccg aaacgagcca gccgcgcgtg cctttcaact tggaatcgta ccaccgctcg
     2341 atgcctccca tgccactggt gatttccgtc aacggcccgt tcgtctgttc ggtcacgccc
     2401 acaaatccga tgacatgcgc cgccaaactg ccgttgggat aaatccgcaa ctggccatcc
     2461 accgggtcag tcatgattcc gccattgcgg aggttccgga aaaacgcctg gtcttttttt
     2521 gaaaattttt gttcatccac gccgaagctc agcttgttca tggcgtcctg gattttctcc
     2581 catgcgtcgg tcgaaacttt gcgtttgagc acgacgtacc gcaccgggac catctgccct
     2641 ttttcattcc gacgcgcgcg cgggacaaga cgccgcgcga gatcgctttc attcatctgc
     2701 aacaacggcg caaggacgcg cgcgacgtca gccgagtaat tgccgatgag ggaaggatcg
     2761 gcgcaaactg ttttcacgaa aacgctcgtc gcgagaagat tgcccttgca atccagtatg
     2821 tcgccgcggc gcggttcaag gcggcgggtg aattccgtgt tgctgcgcgc ttccttcgag
     2881 agttcgccat gccggacgac ctgcaaatcc accaggcgat agccaagccc ggcgaaggcc
     2941 agcgccaata aaaacgtgag cgtaaacagc ctcttgaatt gcagtttctt tgccattctg
     3001 attcccaaaa aattttacca accgcccgcg ccccttcggt tcctcttccg acggtgtcac
     3061 gggcggcggt aaaaaatcat tgctccatca cggcggccac agcgccggtc ctgccggctg
     3121 cgtattcccg ttccggtttc gacgccgccg gaatctccgc cgcgggttcc ggcaaacgcc
     3181 agatttgcgc cggctgcggc ggaacaaggc caagcttcaa ttccctcgcg cgcgcctcga
     3241 gcttcggcgg cgagagcagc atcgcaatct gtttcttgag ctggtcattc gtgtagcgca
     3301 aatcggccag gcgattttcc tggttgcgga tatggtgcga aagctcggct atctgtttct
     3361 tctgccagac atagccgacg cagcagatca aaatgaaaaa acagatcaac gatgccttca
     3421 gcgcgggccc gaccagaagc cccgccgatt gattttttcg gttccgtgcc atttcaaatc
     3481 ttttccattg tcctcaattg cgcgctgcgg ctgcgcggat tttccgccag ttcggccgcg
     3541 cccggtttca ccgctttgcg ctcgatccat ctcaactccg gcttccgtgg ccgccgcaac
     3601 tccggcacat ccaccggccc ttcaaattcg taatcccgcg accgctcgcg tccgaattct
     3661 ttaaccacgc gaacttcaag gctgtggaat gtgatgaccg caagccgtcc gcccggcttc
     3721 aacaacttca ccgccccatc caacccccgc cgcaacgaac ccaactcatc gttcaccgcc
     3781 attcgcaacg cctgaaaaac tttcgtcgcg ggatgcgtcc gctgtccgcg tctcggcgac
     3841 agccgctcga tcaagccggc caattgtccc gtcgttgtga acggccgctg ctgccggtcc
     3901 atttcaattg cccgcgcaaa ccggcgcgat tgcggctcgt cccccagttc ccaaaaaatc
     3961 ttcgccagtt catcggcgct caggcggttc accaggtccg ccgctgtcgt gccctgccgc
     4021 gtgtccatcc gcatgtccag cggaccttcc tgctggaaac tgaaaccgcg ttcggccctg
     4081 tccagttgcg gagaactcac gcccaaatcc agcaacacgc cgtcaaaact tcccggctcc
     4141 cacatctgcg ccatctccgc aaaattcccg cgccgtatct caaaacgccc ggcgaattcc
     4201 gccagccgcg cctgcgccgc ttcaaccgcg gcgccatctc gatcgcagcc acacaaccat
     4261 ccagtcggcg agctcgccgc caatatggct gccgcgtgtc cacccccacc ggccgttccg
     4321 tcggcgtagc gcccgtccgg cctcggcttt agcgcggcca acacctccgc caccatcact
     4381 gggttgtgac tgtaatttgg cacttcgcat ggcccttagc gcaccaacga ttgactcgtt
     4441 ccaaaaatcc gcgacgcaat gcggctgaaa ttgcctgcat ccgcttctgc caacggcgcc
     4501 aaccgcaccg gcggcgcgtc ggccttctcc gactttttga ttgtcacaac ttccaggtcg
     4561 gtctcgttca aatcgtttcg gatcactttg acgcggtcca acgaaagctc agtctgcacc
     4621 ggcttgcgcg cattgatatt ttgcgaacgc ctgccaaatc gaaacaattt aaagagcggc
     4681 gcaagccagc cgaaggtctg ctcactcttc gctggcttgg ccgcgatgcg ggatgattca
     4741 tggtccggcg acggcgtggg cggttcggtc ggcatcggtt gcgccggctg gttggccggg
     4801 acttttggcg gagcaatgac cggcgccggc aacggcgcgc gcgtcggcgt gccggtcttc
     4861 aaacccagcg ggctggcatc caccaaaccc cggttgggtt ccggctgcat cacacggcgc
     4921 tgttcaagca ccggttgaac acttgctttc gcgggcgaca ccttcgctgg cgcgttcaca
     4981 ggaatcgcga cggcttccgt ctcgttcatt ttcgccgcga ctgcagcgac ggctaagatt
     5041 ggtttcagtt caacgtcaaa aagcggataa gtttccatcg gcgcgaccat cacggcgcat
     5101 ggggctgcaa tcggtggcgg agtcgcaggc tcggattttg gcgcttccgt tttgggaatg
     5161 gaagcgaatg gatttttcgt ggattcgaat tttggaagca ggttctccgc gcgcatccga
     5221 taaggactct gcgagttcgt cattccaacc agactctttc ccgccgcaag caatcgtgaa
     5281 aagctcatag tttgttaaat gagtttaaag gcttcaggca aaagggccgc gtccaccgca
     5341 ctcacggttt catatcgttc gggattccag atctcgaacc ggtccaccaa tccaaccagc
     5401 accgcatcat tctcgatgcc gacagctttc gccatcgtgt cgggaatgca gatccttccg
     5461 gccttgtcca gggccacgct cgccgatttg ctgccgatca accgacgcaa cgccgatgct
     5521 ttcggatcgg cgctcgacat cgttcgaatt ttttcagcaa gcgctttcat ctcggtcggc
     5581 ggcagcacca gaagatttgc gtccggttgc ccgccattgg gccagaggat cagcgtgaac
     5641 tccacgtcgg gttctgacgg acgccacttt gccgggattt ggacgcgacg cttctcatcg
     5701 accccatgtc gaaacaggga agtataatga atcgtctcgt tgtcagtgct cggcattttg
     5761 atgtcattcc gtcagaacct caacccacag ttatacacaa cgcgacactt ttaaccattt
     5821 tcccccacag catgtcaata tctatcgtga aaatttttta ttcagaagtt attcacaatt
     5881 cgaaatggta ggtaaatacc gcagtccgca accgccaaag ttgcggtttt taaagggttt
     5941 ttttatggag ggtcaagaaa gctattggat aaagtgactt cctaaaaaaa ttattcccaa
     6001 tgaaatatcg ttaaagaatt cgtgaaaaac tctgcttttg ggaactgttg gcgcaattcg
     6061 gaggcaaaag tctcgttttc gacacgagaa atatctgccg aagtgtggaa tctgtttaca
     6121 ctttaaactt tgaactcgaa actttaaacc aatttgtgct gacaaacgat tttgattttc
     6181 aattgccgcc ggaattaatt gcgcaacaac ccgcgatgcg gcgcgatcaa tcccgcttgc
     6241 tcgttttgca gcgcggttct gggaaattga cccaccgcac ttttcctgac cttctcgaat
     6301 atctgaatcc tggcgatgtc ctcgtgctga acaattcgcg ggtcatcccg gcacggttgc
     6361 gcggcgtgaa cactaagagc cgcgggcaat tcgaaatttt gctgctcgaa gagaacgcca
     6421 ccaacgattg gtgggtgatg ctgcgccccg gcaaacgcgc gcgcgtggga acagaaatta
     6481 ttttccgcga tctcgcagga aataaatccg ccgttcacgc gagagtgatt gagacgaacg
     6541 ccgagggaca tcgccgtctg caattttccg gcgtcgcaga cattcgcgac gtgctcgacg
     6601 cactcggaga aattcctttg cctccgtaca ttgcgcgcgc aaatgccgat gaattggccg
     6661 ccgacaaaat ccgttatcaa acagtttatg ccgcgtcggc cggttccgtt gccgcgccga
     6721 cagcaggatt gcatttcact gaggaattat tgtccgcgat tcgcgcgaag ggtgtgcatg
     6781 tctcctttgt gaccctgcac gtcggcctcg gcacgttcgc tccggtgaaa gccgaaaccc
     6841 tttctgaaca cgtgatgcac gaggagcgtt acgaagtaag cgacgaaacc gcgcggcttg
     6901 tgaacgatgc gaaaaattcc gacaagcgcg tcatcgcggt cggcacaaca tccgtgcgtg
     6961 tcttggaaag tgtggcgcaa aaaaatcagg gccgcatcgt tgccgggaac ggccgcacgc
     7021 gtatttttat ttttccgccc gcagaatttc aaatcgtgga cgcattgctc accaatttcc
     7081 atctcccccg ctccaccttg ctgatgctcg tgagcgcctt cgccgcgccg cgcgaacttc
     7141 gtggacgtga aatgattttg cag
//
LOCUS       DASZMM010000132         7462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3066316, whole genome shotgun sequence.
ACCESSION   DASZMM010000132 DASZMM010000000
VERSION     DASZMM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066316"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..722)
                     /locus_tag="VG754_03275"
     CDS             complement(12..722)
                     /locus_tag="VG754_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18257.1"
                     /translation="MRLRCLGGFFILLMFTASSPCSFARQDSTDLTNFPPGATKEEKL
                     ATVKLEMTNAINRVCEIVNRPVKAYVRQGDIDVSIYSPGWFHPGASKPDFNTVDVRQT
                     QELPYAAHKFVSSDLNPGIVFLGQDLEFNSMTKYFYTNRTVPKRKLTDAEMQEINRLY
                     RVIGKCEQQILEIKAPPENQAAQSGKTGDTEEVIPGQSFESIRSIPRRTRAIYAGIGI
                     GVLLLLVIVMRVVRKRPG"
     gene            1081..5211
                     /locus_tag="VG754_03280"
     CDS             1081..5211
                     /locus_tag="VG754_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF18258.1"
                     /translation="MKPSRFPSPLITIISVIILLFHLGIARADFNPVALTPGSYTFGI
                     VVPSNTVQAVPYCVNVFAGSGTCLSCDTTFYEQGMNNPFPFSGWNSGIPRHGTIFTNI
                     NNANMTFVMPPDYTTNNDLMIVNSGSYGTINSGTLNFVTPTTATKLAILNTGGNGGAT
                     VTYTVTHSDNSTDTGSIVVHNWFDSSTTIAWGANGRMDQGGGFDNFNSSSVNNNPPYL
                     YANVINVSSNTPIVSITFNYSSGAGVDNFYAVSGVNVGTVYTPIPVSGFNVVSTIPVL
                     APLPVNATMDNGTNLIPANGGLGNTWFEQGYYSNNASYGVPPSGSTFDSFSQPTHHYQ
                     MGNYSSDNAILIDTNHQLANITPVTPAAYTGLAFLTAGGDIGTGNKMTNICILQHQDG
                     VNETNIFFGYDWFESTVPGAIAYEANGRVYLNNRTLNNLGNQGFPYMFETYFTLTDLS
                     SPITNIVVKFKASPSVQATTFIMAISGTTNAIAPVITQEPLPTTQTWYPGQTANISVL
                     VAGSQPITNIWLVESNGIYVPLSDGLDANGSYISGSSTTTLTISNLQAGDGTSYEYVA
                     TNAVGGMTSTPATITVNSGTPSGPIIDSQTPTASFSVLTNHPETTTFSVTIDASSAPP
                     LYYQWYTVSPDNVTNPIPGATATTYVNVDTNNQTIFCVVSNFVSTASSTPITINIFLP
                     PAPSQYESALLAYHPVGYWPLSETNGTVAFDYAGTNDGTYTGSYTLGQPGLPPTPGIG
                     ANTSVGFDGSTAYVAIPGAGSLNITGPMSIIQFVQPPSGGDTGFTTSVGHSDQSYRLD
                     VVGGQPHFADAGPDIVDSATINDGNWHLLVGVYDGTNEYLYVDGQLVATPRASTPSGS
                     TDNVTIGAAPDYLGARNFNGNIAQVAILTNALTAAQVSALYGNLDTAPTVSVTPANPS
                     IYSGGSVTFTAILGGATPASSLQWYVIDTSNNSNNIPGANGNTYTIASAPITDNGNTY
                     GIIAANAYGTNTASAVLTVQNGPAYLPSGGDISPLTAEAYVGAPVTYTVHAGGSLPIS
                     YQWTVNGTNVSGATSSSFTFPAPCGASTIQASFTNAQNGGTPVLSSQAALQGDALPSI
                     ITFNTNGTGWQLNLQPGNSTVPTLTSNVLELTDNGGGEASSAFYQTAEYVGNFSAAFT
                     YTAGGDAAADGVAFILQNSSAAATALGGGGGDLGYYQITNSVALELNIYFNNGVGIAF
                     GTNGNTLGSGLGAPYAPTGGVVLNSGDPIDFKLNYANGNLSVQLTDSTIPATFTTNYA
                     VGPLTSLLGGTDLAYVGFSGGDGGAVSIQTISNFAFNSVIPPVSLSVSSVSANSFVLS
                     WPATGTDYYQLQTATSLTGPWSAGPTPTTVGGVNKVTILTNSGSHQFYRLVRIVCQ"
     gene            complement(5321..6088)
                     /locus_tag="VG754_03285"
     CDS             complement(5321..6088)
                     /locus_tag="VG754_03285"
                     /inference="COORDINATES: protein motif:HMM:NF020927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HWF18259.1"
                     /translation="MQSTSLPVIDRTTLLAGLREKISARQPIIGRHLIPQSEEGAVPP
                     DLFALYHTAKFPQHGLMGGLLAFGDANKIVLELAGDALAEANGAPVLAGVSAADPFRL
                     IPIFLKEIKEAGFAGVQNFPSMGIMDGAFRSHLESSGGGYNKEIELIRLAREFDLLTA
                     PLVFNTDEAARMTEAGADILVINAPLNASSYLKRKNAGEQKDVLEGLKRTVEAAKKIR
                     PDVILLFGSYWPDDLKNARSHYDSCPGLDGYWTTGRE"
     gene            complement(6101..>7462)
                     /locus_tag="VG754_03290"
     CDS             complement(6101..>7462)
                     /locus_tag="VG754_03290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWF18260.1"
                     /translation="AGLGFEREVALLSQARENGLLTIGFCLTEDEAVALCRANVHILC
                     LGLGFAEWQRDDQARHQAALDESIAFINRVIAATKKVSSKPYLTVLGGPVLLPQDTAQ
                     VYNRTEALGYIGGSTVERFPAAPMITQTVSEFKQATHAGRAPERLGSLVGRSPAMQKV
                     FDTVRRVAKFHAPILIIGESGTGKELVAREIHRLSARHSRPLVSWNCGAMTESLATSE
                     LFGHEKGSFTGATRTHLGRFEVAHGGTLFLDEITDLPLAVQASLLRVLQERELVRVGG
                     EKSIPTDVRLIAASNKNFRELIPTGRFRLDLYYRLNTVVLELPPLRERREDIPILISE
                     ILQELSLQYGCSLPLIPEKMMEMLVAHSWPGNIRELRNVLERCFILGEGRVLSPTWLA
                     DAFSSNNFSNEPAGEIAISKLPRRARRDHLIEVLARHDGNKVAAARDLGVARKTIYNW
                     LRG"
BASE COUNT         1652 a         2154 c         1965 g         1691 t
ORIGIN      
        1 gcgaattgag gtcagccagg tcttttcctc acaacgcgca tcacaatcac gagcagcagt
       61 aatacgccga tgcctatgcc ggcgtaaatc gcccgggttc gtcgcgggat gctgcggatg
      121 ctttcgaagg actgtccggg aatgacttct tcggtgtcgc cggtcttgcc cgattgggcg
      181 gcttggtttt caggcggggc tttgatctcc aagatttgct gttcgcactt gccgatgacg
      241 cggtagagcc ggttgatttc ctgcatctcg gcgtcggtga gcttccgctt gggcactgtg
      301 cggttggtgt aaaaatattt tgtcatcgaa ttgaattcca agtcctggcc gaggaacacg
      361 atgcccgggt tcaaatccga gctgacaaat ttgtgcgccg cgtaaggaag ctcctgcgtt
      421 tggcgcacgt ccaccgtgtt gaaatccggc ttgctcgcgc cgggatgaaa ccatccggga
      481 ctatagatgc ttacgtcaat gtctccctgc cgcacgtaag ccttcaccgg acggttcacg
      541 atttcgcaaa cgcgattgat ggcattggtc atctccaact tgacggtggc cagcttttcc
      601 tccttcgttg cgccgggagg aaaattcgtc aaatcagttg aatcctgccg ggcgaacgag
      661 cacgggctgc tcgccgtgaa catcaacaaa atgaaaaagc cgccaaggca tctcaaccgc
      721 atgactgcat gtttccaagc ttctgcatca aggtcaacat tgtccggcaa agagggaaat
      781 cttgttttgt tttaggacaa gctaattcgt cttgttcgtt tctcatatct gtcaaatcct
      841 cgacccaagc ccagagatgc atctcgataa aagttaaacg ttcaggtttt tggcgagccg
      901 agtctcaaaa gattttttgc gatgtcacgg cgcgtcacgg agtcatgacg catttgcgaa
      961 aacagcggaa ttgctgtaca tggaatacgt tcaccctgac ctgaatctga gaaagagccc
     1021 gtttacatta gtgatgtccg gatcacgttc aaccccatca accatatctc tattatcccc
     1081 atgaagcctt ctcgtttccc atcacccctg attacaatca tcagtgttat aatcttgttg
     1141 tttcatctcg gcattgcccg ggccgacttc aacccagtcg ccctgacgcc gggaagttat
     1201 acgtttggaa ttgtcgtgcc atccaatacc gtccaagctg tgccttattg cgtgaatgtg
     1261 tttgcagggt caggaacctg cctttcgtgt gacaccacat tttatgaaca gggaatgaac
     1321 aacccgttcc cattctccgg atggaattcc ggcattcccc gtcacggaac cattttcacg
     1381 aacatcaaca acgcgaacat gacgtttgtg atgccgcccg attataccac gaacaatgat
     1441 ttgatgattg tcaacagcgg aagttacggc accattaaca gtggcacact gaatttcgtc
     1501 accccaacaa ccgccacaaa gcttgccatt ctcaatacag ggggtaacgg gggcgcgacc
     1561 gttacctata ctgtcaccca ttctgacaat tccaccgata cgggatccat cgttgtgcat
     1621 aactggttcg atagcagtac gactatcgct tggggtgcga atggtcgcat ggatcagggc
     1681 ggtgggtttg ataattttaa cagcagctca gtaaacaaca accctcctta tctctacgcc
     1741 aatgtgatca acgtctccag caatacgccc attgtcagca tcacattcaa ttatagttct
     1801 ggcgcgggtg ttgacaattt ttacgccgtc agcggcgtca acgtcggcac agtttacacg
     1861 ccaattccgg tgagcggctt caatgttgtc agcaccattc cagtcctcgc gccgttgccg
     1921 gtcaatgcca ccatggataa cggcaccaat cttattcccg ccaatggagg cctcggcaat
     1981 acatggttcg agcagggtta ttattcgaat aatgcgagct atggagtgcc cccttcaggc
     2041 tcaactttcg acagcttcag ccagccgacc catcattatc agatgggcaa ttactccagc
     2101 gacaacgcga tcctcatcga tacaaatcat caattggcca acatcacgcc ggtcactccc
     2161 gccgcttata ccgggctggc atttttgacg gcgggcggcg acatcggcac cggaaacaaa
     2221 atgaccaata tttgcatcct gcaacaccag gacggagtca atgaaacaaa tattttcttc
     2281 gggtacgact ggttcgaatc gaccgttccc ggcgccatcg cttatgaagc caacggccgg
     2341 gtgtacttga ataaccgcac cttgaataat ttgggcaatc agggtttccc ttacatgttt
     2401 gaaacgtatt tcacactcac ggacctctcc agtcccatca ccaatattgt ggtgaaattc
     2461 aaggcatctc cttctgtcca agccacaacc ttcatcatgg cgatcagcgg cacgacaaat
     2521 gccatcgcgc ctgtgatcac tcaggaacca ctacccacca ctcagacatg gtatccaggc
     2581 cagactgcga acatctcggt cctggtcgcc ggcagccagc ccatcaccaa tatctggctg
     2641 gtggaaagca acggcatata cgttccgttg tcggatggac ttgacgccaa tggctcttac
     2701 atctccggct cgtccacgac cacccttacc atcagtaacc tgcaggcggg cgatggcacc
     2761 agttacgaat atgtcgcgac gaatgctgtt ggcggcatga ccagcacccc ggcgacaatc
     2821 accgtcaatt ccggcacacc gtccgggccg atcattgatt cccaaactcc gacagcctcg
     2881 tttagcgtcc tgacgaacca ccccgagacc acgacgtttt ccgttactat tgatgcctcc
     2941 agcgcgccgc cgctctatta tcaatggtac actgtttcgc ctgataacgt cacaaatccg
     3001 attccgggcg caaccgctac gacttatgtg aatgtggata cgaacaatca aaccatcttt
     3061 tgcgtcgtct ccaacttcgt gagcacagcc agcagcacac cgataacgat caacatcttc
     3121 ctgccgccag ccccgtctca gtacgagtct gcgctgctgg cttatcatcc tgtcggctat
     3181 tggcctctca gtgaaaccaa cgggaccgtc gccttcgatt atgccggcac aaatgacggc
     3241 acttataccg gcagttacac gttgggccag cctggtcttc cgccgacacc cggcatcgga
     3301 gcaaacactt ccgtgggttt cgacggcagc acggcgtatg ttgccattcc gggagcggga
     3361 tcgcttaaca tcacaggccc gatgtccatc attcaatttg tccagcctcc ttcgggcgga
     3421 gacaccggtt tcacaacgtc cgttgggcat tcggatcaga gttaccgact ggacgtcgtc
     3481 ggtggacaac cgcacttcgc tgatgccgga ccagacattg ttgattccgc cacgatcaac
     3541 gatggcaatt ggcatctatt agtgggcgtg tatgatggca cgaacgaata tttgtatgtg
     3601 gacggccagt tggtggcgac gccaagagcg agcactccgt ccggaagcac ggataatgtg
     3661 accatcggcg ccgctccgga ttatctgggc gcccgcaact tcaacggcaa catcgctcaa
     3721 gtggccattc tcaccaacgc attgacggca gcgcaggttt ccgcgcttta tggaaacttg
     3781 gacaccgctc cgacggtttc agtcacgcct gccaatcctt ccatctattc cgggggcagc
     3841 gtgacgttca ccgcaattct ggggggcgca acaccggcgt ccagcctgca atggtacgtc
     3901 atcgatacgt ccaacaattc caacaatata ccgggtgcga atggcaacac ctacaccatt
     3961 gccagtgcgc ccatcacgga taatgggaac acttacggca tcatcgcggc gaatgcttat
     4021 ggcacgaaca cggcctcggc ggtattgacg gtgcaaaacg ggcctgccta cctgccatcc
     4081 ggcggcgaca tctcgcccct gaccgccgaa gcttacgtcg gagcgccggt gacttacacc
     4141 gttcatgctg gcggaagctt gcccatctca tatcaatgga ctgtgaatgg caccaacgtg
     4201 agcggggcaa ccagttcctc ctttacgttc cctgcgccat gtggagcgag cacaattcaa
     4261 gcaagcttca cgaacgcgca gaacggcggt actcccgttc tcagttcgca agccgcgctt
     4321 caaggcgatg ccttgccgtc gataatcacc ttcaacacca acggcacagg ctggcaactt
     4381 aaccttcaac ctggcaacag caccgtaccc accctcacat ccaatgtgtt ggaattgacg
     4441 gataacggcg gcggcgaagc atccagtgcg ttttatcaga ccgcggaata tgtgggcaac
     4501 ttcagcgcgg cattcaccta cacagccggc ggtgatgcgg cagccgacgg ggttgccttc
     4561 atcctccaga acagttccgc tgcggcgacc gccttgggcg ggggtggcgg cgacttgggt
     4621 tattatcaaa tcaccaacag cgtcgcattg gaactcaata tttatttcaa taacggtgta
     4681 ggcattgctt ttgggaccaa cggcaatacc ctcggttcgg gactcggcgc tccttacgcg
     4741 ccaaccggtg gggtggtgct gaacagtggc gatcccatcg actttaagtt gaattatgcc
     4801 aacggcaatc tgtcggtgca gttgacggat tccaccatcc cggcaacttt caccacgaac
     4861 tatgcggtcg gcccgttgac ctcactgctt ggcggaacag acctggctta cgttggattc
     4921 agcggcggtg atggcggggc cgtttccatc cagaccatca gcaatttcgc cttcaactcg
     4981 gtgataccgc ctgtgtctct gtcggtctca tctgtgagcg ccaactcctt cgtgctttcg
     5041 tggcctgcga ctgggactga ttactaccag ttgcaaacag ccacaagttt gacaggccct
     5101 tggagcgctg gcccgacccc gaccacggtt ggcggagtca acaaggtcac catcctgacc
     5161 aacagcggct cacaccagtt ctaccgcctc gtgcgaattg tctgccaata gcctggaact
     5221 gttaagctga cttaaaacgc gagatgtgcc gccacttggc ggcgcatctt tttttccggc
     5281 gatgcgaagg cagaagtgaa atcaattgcg aggttctaaa tcactcgcga ccggtagtcc
     5341 aataaccatc caagccggga catgagtcgt aatgcgaacg ggcgtttttg aggtcgtcgg
     5401 gccagtagct gccgaagagc agaatcacgt cgggtcgaat ttttttagcg gcttcaacgg
     5461 ttcttttgag cccttcgaga acgtcttttt gttcgccggc atttttgcgt ttgagataac
     5521 tggacgcgtt cagcggagca ttgatcacga gaatgtcggc accggcctcg gtcatgcgcg
     5581 cggcttcatc ggtattgaag acgagcggcg cggtgagcag gtcgaattca cgggccagcc
     5641 ggatcaattc gatttctttg ttgtatccgc cgccgctgct ttccaagtgg gaccggaacg
     5701 cgccgtccat gatgcccatg ctcggaaaat tctggacccc ggcaaacccg gcctccttga
     5761 tttccttcaa aaaaatcggg atcagccgga acggatcggc agccgagacg ccggctagaa
     5821 cgggcgctcc gttcgcctcg gccaaagcat ctccggcgag ttccagcaca attttgttcg
     5881 cgtcgccaaa tgccagcaat cctcccatca atccgtgctg cgggaatttg gccgtatgat
     5941 aaagagcgaa caaatcggga ggcacggcgc cctcttcgga ttgcggaatc agatgcctgc
     6001 cgatgatggg ctgacgcgcg gagatttttt cacgcaatcc agcaagcaac gtcgtccgat
     6061 ctataactgg cagagaagtg ctttgcatga ttgaaaaatt tcagccccgc agccagttgt
     6121 agattgtttt gcgggcgact ccgaggtcgc gggcggcggc gactttgttg ccgtcgtgcc
     6181 gtgcaaggac ttcgatcaag tggtcgcgcc tggcgcggcg cggcagtttg gaaattgcga
     6241 tttcccccgc gggttcgttc gagaagttgt tggaggaaaa tgcgtccgca agccaggtgg
     6301 gagaaagcac gcgtccttcg cccaggatga aacagcgttc aaggacattg cgcagttcgc
     6361 ggatgtttcc cggccatgaa tgggcaacga gcatctccat cattttttcg ggaatgagcg
     6421 gcagggaaca gccgtattgc aggctcaact cctgcaggat ttcggagatc aagatgggaa
     6481 tgtcctcgcg gcgctcgcgc aacggtggca actccagcac aacggtgttc aagcgataat
     6541 aaagatcgag ccggaaacgt ccggtgggaa tcaactcgcg aaaatttttg ttcgatgccg
     6601 cgatgaggcg gacatcggtg gggatgcttt tctcgccgcc cacgcggacc aattcccgtt
     6661 cctgcaaaac ccggagcaaa ctggcttgga cggcgagcgg caggtcggtg atttcgtcca
     6721 gaaaaagtgt gcctccgtgc gccacttcga atctcccaag gtgcgtgcgc gtcgcgccag
     6781 tgaatgaacc tttctcgtgg ccgaacaatt ccgaggtggc aaggctttcg gtcatcgcac
     6841 cgcaattcca actgacgagc ggacgcgaat gccgcgcgct caaccggtgg atttcgcggg
     6901 cgacaagttc ctttccagtc ccgctttcgc cgatgatgag gatgggcgcg tgaaattttg
     6961 cgacgcggcg gacggtgtca aacacctttt gcatcgcggg cgaacgaccg accagcgagc
     7021 cgagccgttc gggcgcccgc ccggcgtggg tggcttgttt gaattcgctg acggtctgtg
     7081 tgatcatggg cgcagcggga aatctttcga ccgtgctgcc gccgatgtaa cccaacgctt
     7141 ccgtgcgatt gtaaacttgc gcggtgtcct gcggcaggag caccgggccg cccaacacag
     7201 tcaagtaagg ctttgaagat actttttttg tggcggcgat gacgcggttg atgaaggcga
     7261 tggattcatc aagggcagcc tgatgcctcg cttgatcgtc gcgttgccat tcggcaaatc
     7321 cgaggccaag gcaaaggatg tgcacgttcg cgcgacaaag cgccaccgct tcgtcttccg
     7381 tcaggcaaaa tccgatggtg agcagtccgt tctcgcgcgc ctgcgagagc aacgcgactt
     7441 ccctttcaaa accaagaccg gc
//
LOCUS       DASZMM010000133         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4991350, whole genome shotgun sequence.
ACCESSION   DASZMM010000133 DASZMM010000000
VERSION     DASZMM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4991350"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VG754_03295"
     CDS             complement(<1..282)
                     /locus_tag="VG754_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18261.1"
                     /translation="MKRTFSLIALGLLAIGGALFTGCETSHDTGAYVPVNTTINDVEN
                     HQLVVLLDPAVQYSVTCSGIQQRYTPDGRLDVVANIRNREGRRIQVQINC"
     gene            complement(279..1460)
                     /locus_tag="VG754_03300"
     CDS             complement(279..1460)
                     /locus_tag="VG754_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18262.1"
                     /translation="MKRFFPAFSAGLLLLVSGCVTEKVHQINLTGDIMVDGPRAIEEG
                     PARDRSLWEYRVAAAAMRRGDYATAKKMLDDALLTLGGIYGKDANAKKARSYFTEENK
                     KTFIGEPYERVMAYYYRGILYWMDGEPDNARACFRTGELEDSSSVGEQYNADYVLLDY
                     LDGLATTKLGDDGSDAFQRAEKLSTHFAKPPSYDKKANVLLFVEFGPGPRKYATGPYR
                     EELRFSVPDSPVRTAIVKAEGQTITLGPYDDLGFQATTRGGRVMDHVLANKAVFKGAT
                     DAAGNAAIIGGAIAASSRNQTSDEVGLGLIAAGIISKVVSATTTPAADIRTWDNLPRY
                     LTFAALELPPGPHTVTVEFQDSFGGPMPSLTKTLNINVPADNHDKVVFVSDASTTPQT
                     L"
     gene            complement(1488..2123)
                     /locus_tag="VG754_03305"
     CDS             complement(1488..2123)
                     /locus_tag="VG754_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HWF18263.1"
                     /translation="MNLTLNKFCSSALMLATAGIIAGCASSGVKNPSGVPTTEMRPDE
                     RGFVAGTGIESQDMVSVTDKMARSILGTPQIANAQGTPCVVLDPVKNETRFPINKDIF
                     LTRIRAELSKKAAGKVWFLARDRMETLERERELKQSGQVTASANPNQVEFKGADFFLT
                     GTLQSMTSRTGAGTSDYVLYDFQLIDARSGVSVWEDSAEIKKQGLEDAAYR"
     gene            2469..3242
                     /gene="hisF"
                     /locus_tag="VG754_03310"
     CDS             2469..3242
                     /gene="hisF"
                     /locus_tag="VG754_03310"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWF18264.1"
                     /translation="MLTRRVIPCLDVHGGQVTRGVQFGKAEAGGLRNVGDPVELALRY
                     NEQGADEMVFFDITATAHGRASIINVIERTADQCFMPLTVGGGIRSVDDMYAMLRAGA
                     DKISINSSALANPELIRAGAEKFGSQCIVVSIDAKKVSRERWEVFSHGGRKATGLQAV
                     DWAKRAVALGAGEIVLNSIDADGTKAGFDLVITRRITESVGVPVVASGGAGTLEHMAA
                     VLLEGKADAVLAASIFHYGTYTVGDVKQFLAKQNLPVRL"
     gene            3292..>4359
                     /locus_tag="VG754_03315"
     CDS             3292..>4359
                     /locus_tag="VG754_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWF18265.1"
                     /translation="MKSLLALGFAFFASLSVFANPPNILYIMSDDHAAHAISAYGSKI
                     NQTPNIDRIAHEGMRFDDCFCVNSICSPSRATILTGKYSHMNGVPVFNRFDGSQPTVA
                     KYLQAAGYYTGMIGKWHLGSDPTGFDKWTILPGQGVYFDPVFIEPTGRRTIPGYVTDI
                     ITDLSINFLKNRPKDKPFFLMCHHKAPHRNWQPDEKHKAMFAKLTIPEPGTLHDDYAT
                     RTDAIRECTQKVFQDLTRRDLKLEPPADLTEKERNEWLSVKPTEVEIQQDGKSVKLTS
                     DALNAWKYQRFLQDYLAVVQSIDDNVGRLLDWLDQNGLSSNTIVIYTSDQGFFLGDHG
                     LFDKRFMYEASVRMPFLVRWPG"
BASE COUNT          923 a         1219 c         1249 g          968 t
ORIGIN      
        1 gcaattgatc tgcacctgga tgcggcgtcc ctctcggttg cggatgttgg cgacgacatc
       61 cagccggccg tcgggcgtgt agcgttgctg gatgccggaa caggtgacgg agtattgcac
      121 cgccgggtcg agcagcacga caagctgatg gttttccacg tcgttgatgg tggtgttcac
      181 cggcacgtaa gcgccggtgt catgcgaggt ttcgcagccg gtgaacagcg cgccgccaat
      241 cgcaagcagg ccaagggcga tgagactgaa ggttcttttc ataacgtttg gggagtggtc
      301 gaagcgtcgc tgacaaacac aaccttgtca tgattatcag caggcacatt gatgttgagc
      361 gttttggtca gagagggcat cggcccgccg aagctgtcct ggaattccac cgtgacggtg
      421 tgcggaccgg gcggcaattc gagcgcggcg aaggtcaggt aacgcggcag attgtcccag
      481 gtgcggatgt ccgctgcggg cgtggtggtc gcggaaacaa ccttgctgat gatgccggca
      541 gcgatcaagc caagcccgac ttcgtcgctg gtctggttgc ggctcgacgc ggcaatcgct
      601 ccgccgatga tggccgcatt accagcggca tccgtggcgc ccttgaagac ggccttgttc
      661 gccagcacgt ggtccatcac gcgcccgccg cgggtcgtcg cctgaaaacc aagatcgtca
      721 tacggcccga gcgtgatggt ctggccttcc gccttgacga tcgccgtgcg aaccggcgag
      781 tcgggaacgc tgaaacgaag ttcctcgcgg tacgggccgg tggcgtattt gcgcggcccg
      841 ggaccgaatt cgacaaacag caggacgttc gcctttttgt cataagacgg cggcttggca
      901 aaatgcgtgg aaagcttttc ggcgcgttga aaagcgtccg agccgtcgtc acccagcttg
      961 gtagtggcga ggccatcgag ataatcgagc aacacgtaat cggcgttgta ttgttcgcct
     1021 acagaagagc tgtcctccag ttcgccggtg cggaagcagg cgcgcgcgtt gtccggctcg
     1081 ccgtccatcc agtaaaggat gccgcgataa taataggcca tcacgcgttc gtaaggttcg
     1141 ccgatgaacg tcttcttgtt ttcctcggtg aaataactgc gggctttttt ggcgttggcg
     1201 tccttgccgt aaatgccgcc gagggtgagc aaggcgtcat cgagcatttt cttggcggtc
     1261 gcataatcgc cgcggcgcat tgcggctgcg gcaacgcgat attcccagag cgatctgtcg
     1321 cgggcgggcc cttcctcgat ggcacgcgga ccgtccacca tgatgtcgcc ggtcagattg
     1381 atctgatgca ctttctcggt cacgcaaccc gacacgagca gcagcaaccc ggcgctgaaa
     1441 gcgggaaaaa atcttttcat tgcttgaagt cggcaaacac gatgggctta gcgataagcc
     1501 gcgtcttcca acccctgttt cttgatctcg gcggaatctt cccaaaccga gacgccgctg
     1561 cgcgcatcga tcagttggaa atcgtaaagc acataatcgc tcgtgcctgc gcccgtccgg
     1621 gaagtcatgc tttgcaatgt gcctgtcagg aaaaagtccg cgcccttgaa ttcgacttga
     1681 ttgggattgg cgctcgcggt gacctggccc gattgcttca actcgcgctc gcgttccaag
     1741 gtttccatgc ggtcacgggc gaggaaccag actttgccgg cggctttctt gctcaattcc
     1801 gcgcggatgc gggtcaggaa aatgtccttg ttgataggga atcgcgtctc gttcttgacg
     1861 ggatcgagga cgacgcaggg cgtgccttgc gcgttggcga tctgcggcgt gcccaagatg
     1921 ctgcgcgcca ttttgtcggt gacagaaacc atgtcctgcg attcgatgcc ggtcccggcg
     1981 acgaaaccgc gttcatccgg gcgcatttca gttgtgggca cgccggaagg atttttcacg
     2041 cccgaagagg cgcaaccggc gatgattccc gccgtcgcaa gcatcagggc cgaactgcag
     2101 aatttattca aagtcagatt cataagtttt taagtttaaa gcagattggt tcacggttcg
     2161 caaccgatat gtttatgcgc tcaatgacca gacaacggac cccggcgttc aaaaacgccg
     2221 atgacctttg catgagcggt tctcattctg ttccgtacgg ttggacgcaa tcgtgtcatc
     2281 tttattcgtt gaccgcgaga gggatggttt ggttacaacg caagctgttg ataaataccc
     2341 aattgcccaa gcatccaaat gcccaaagaa ttccgaagca tgaaatccca aacttttcat
     2401 tggcttgtca ccaatggttt tgcgcattaa atataccgcg atgaatttgt tccgtcccgt
     2461 ttaacgccat gctcacccga cgtgtcatac cgtgtctcga tgtccatggc ggccaggtca
     2521 cgcgcggcgt gcagtttggc aaggccgaag cgggtggttt gcgcaacgtg ggtgatcccg
     2581 tcgaacttgc gctccgttac aacgagcaag gcgcggacga aatggttttc ttcgacatca
     2641 ccgcgaccgc tcacggacgc gccagcatta tcaatgtcat cgaacgcacc gcggaccaat
     2701 gtttcatgcc gctcaccgtc ggcggcggca tccgttcggt ggacgacatg tatgcgatgc
     2761 tgcgcgcggg cgctgacaag atcagcatca attcgtccgc gcttgcgaac cccgaattga
     2821 tccgcgccgg cgctgaaaaa ttcggcagcc aatgcatcgt cgtttcgatt gacgccaaga
     2881 aagtttcgcg ggaacgctgg gaggttttca gccacggcgg acgcaaagcg accggattgc
     2941 aagccgttga ttgggccaaa cgcgctgttg cgttgggcgc gggcgagatc gtgttgaaca
     3001 gcattgatgc cgatggcaca aaagccggtt tcgatcttgt catcacacgg cgcatcaccg
     3061 aatcggtcgg cgtgccggtc gtggcgagcg gcggcgcggg gactttggaa cacatggcgg
     3121 cggtgttgct tgaaggaaaa gctgacgccg ttcttgctgc gagcattttt cattatggca
     3181 cctacacggt cggcgatgtg aaacaatttt tggcaaaaca aaatttgccg gtgaggttgt
     3241 aattgccttg aaaattggcg cgctgaaaga caatcattga attcatcatc catgaaatct
     3301 ctcctcgctc ttggtttcgc tttcttcgcc agcctttccg tttttgcaaa cccaccgaac
     3361 atcctttaca tcatgagcga tgatcatgcg gcgcacgcca tcagcgctta cggcagcaag
     3421 atcaaccaga ccccgaacat tgaccgcatc gcgcatgaag ggatgcgttt cgacgattgt
     3481 ttctgcgtca attccatctg ctcgccaagc cgcgccacga ttctcaccgg caaatacagc
     3541 cacatgaacg gcgttcccgt gttcaaccgc ttcgacggct cgcaaccgac cgtcgccaaa
     3601 tatctgcaag ctgccggtta ttacaccggc atgatcggca aatggcatct cggcagtgac
     3661 ccgaccggtt ttgacaagtg gaccattctc ccgggacaag gtgtttattt tgatccggtt
     3721 ttcatcgagc cgaccggacg acgaacgatt cctggttacg tcaccgacat catcaccgat
     3781 ctttcaatca attttttgaa gaaccggcca aaggacaaac cgttcttcct catgtgccat
     3841 cacaaggccc cgcatcgcaa ctggcagccg gacgagaaac ataaggcaat gtttgccaag
     3901 ctcaccattc ccgagccggg gacgttgcat gatgattacg ccacgcgcac cgatgccatc
     3961 cgcgaatgca ctcaaaaagt ttttcaagac ttgacgcgcc gcgatctcaa gctcgaacct
     4021 cccgccgatt tgaccgagaa agagcgcaac gaatggctca gtgtcaaacc gaccgaagtg
     4081 gaaatccagc aggacggcaa gagcgtcaag ctcaccagcg atgcgttaaa cgcatggaaa
     4141 tatcagcggt ttttgcagga ttaccttgca gtggtgcagt ccattgatga caacgtgggg
     4201 cgtttgctcg attggctcga ccaaaacggt ttgagcagca acacgattgt catctacacc
     4261 agtgaccagg gatttttcct tggcgaccac gggcttttcg acaagcggtt catgtatgaa
     4321 gcgtccgttc ggatgccgtt ccttgtccgc tggccgggc
//
LOCUS       DASZMM010000134         5416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4992273, whole genome shotgun sequence.
ACCESSION   DASZMM010000134 DASZMM010000000
VERSION     DASZMM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4992273"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VG754_03320"
     CDS             complement(<1..180)
                     /locus_tag="VG754_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18266.1"
                     /translation="MTDIFLRNRNSLVSPRDILYQYSMTGEILELPLNPTAPRVRHVR
                     MADFASELAGFREFGT"
     gene            complement(373..855)
                     /locus_tag="VG754_03325"
     CDS             complement(373..855)
                     /locus_tag="VG754_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18267.1"
                     /translation="MKQSSDQEPPNRQNPSPGVHIHLGESNIVLLTVTTKKREPWLAN
                     ETAHQLLRQTWSQASAWLVGDYLLMPDHLHVFCAARDLHFDIETWISFWKREFRLKHG
                     RSDWRFQSRGWHHRLREGQHYSEKWIYVQENPLRKQLVQRIEDWPFKGRVFDLVWSGK
                     "
     gene            1580..1852
                     /gene="rpsP"
                     /locus_tag="VG754_03330"
     CDS             1580..1852
                     /gene="rpsP"
                     /locus_tag="VG754_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWF18268.1"
                     /translation="MAVKIRMKRVGTKNTPVYRIVVADGRSPRDGKFIEEIGTYTPLK
                     KDANFTLDLERANYWVSKGAQPSDTVASFIKKAAKAAAAAPATAAA"
     gene            1873..2118
                     /locus_tag="VG754_03335"
     CDS             1873..2118
                     /locus_tag="VG754_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWF18269.1"
                     /translation="MQAFLEYVVKGLVGHPEDVTVTPVEREGMTIYELRLNPDDVGKV
                     IGRQGMTINAIRSLLLAGSAKKGMRCSLEIVEEQPAR"
     gene            2181..2861
                     /gene="trmD"
                     /locus_tag="VG754_03340"
     CDS             2181..2861
                     /gene="trmD"
                     /locus_tag="VG754_03340"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWF18270.1"
                     /translation="MKIDVLTLFPAMFAGPLDESIVQRARKNGALDLAVHNLRDYTHD
                     RHKTVDDRPFGGGPGMLLKPEPLFEAVENLGREKSRVILLSPAGRKFDQAIARELAQA
                     EHLILVCGSYEGFDERIRESLADDELSIGDFVLTNGALPAMMIIDAVTRLLPGVLGDD
                     ESSLDESFSKDLLEYPHYTRPAEFRGMKVPDVLLSGNHAEIAKWRAEQARLRTAERRP
                     DLLKRKTE"
     gene            2930..3349
                     /gene="rplS"
                     /locus_tag="VG754_03345"
     CDS             2930..3349
                     /gene="rplS"
                     /locus_tag="VG754_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWF18271.1"
                     /translation="MNQALLDKIESEQYRKNPVTFAVGDSVKVHTKVVEGEKERIQVF
                     AGVVIGRRGHGLNETFTVRRISYGEGVERVFPIHSPRVDKVEVERKGSVRRAKLTYLR
                     KRLGKGATLVKEKETRGEAASKAPSKAKAAAAEPVTA"
     gene            complement(3401..4180)
                     /locus_tag="VG754_03350"
     CDS             complement(3401..4180)
                     /locus_tag="VG754_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18272.1"
                     /translation="MKAEGKNKDPTQRFSSRVENYIRYRPDYPPAIIPFLERECGFLG
                     SNLVADIGSGTGKLTELFLKHGNRVIGIEPNREMREAAERLLVSYPLFQSINAAAEAT
                     GLEMTSVDMITAGQAFHWFDREKARREFLRILKPGGWLALIWNDRNISARPFFQAYEN
                     LLLTYGTDYEKVGHKHADAQMIGSFFGDSGFKLASFPNEQVFNFEGLQGRLMSSSYAP
                     EAGHLKHAPMVEALAQIFKEHQKDGKVTFEYDTTLYYGRLR"
     gene            complement(4323..4457)
                     /locus_tag="VG754_03355"
     CDS             complement(4323..4457)
                     /locus_tag="VG754_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18273.1"
                     /translation="MFKQLAELVTQLLFLARDTRENKEAIKQLRGEVDELATVVERDL
                     "
     gene            complement(4438..4644)
                     /locus_tag="VG754_03360"
     CDS             complement(4438..4644)
                     /locus_tag="VG754_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18274.1"
                     /translation="MKTKAATASKFNGDFGEYAVIIRDGLKELAAIHRANKTDDAEIR
                     RLQSSTRKKLDRIWKNLRHVQTIG"
     gene            complement(4699..>5416)
                     /locus_tag="VG754_03365"
     CDS             complement(4699..>5416)
                     /locus_tag="VG754_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWF18275.1"
                     /translation="QRCPDDSKAYEFYQIIDKLEREAPDESKKKLAALGFSFDEITAF
                     IRTGKPTAELEAILNNLAARGLSDFVKVDYQVIRGLAYYTGVVFEAFDQKGEFRAIAG
                     GGRYDNLVKLISGGKVNLPALGFGLGDVVLLELLKARDLLPKFDAGLDAFVLIEDEAL
                     RPQSLKLIQDLRSAGYVVEYSLTPAKPDKQFKRAQELRAAFTIKIENASDARIKNLET
                     REERLLLIDEATRHFSEKKT"
BASE COUNT         1237 a         1384 c         1537 g         1258 t
ORIGIN      
        1 agttccaaat tcgcggaagc ccgcaagttc cgaggcaaag tcggccatgc gtacgtggcg
       61 tacgcgaggc gcggttggat tgaggggcaa ttccaaaatt tcaccggtca tgctgtactg
      121 atacaaaata tcccttggag aaaccagcga attgcggttg cggagaaaaa tgtcagtcaa
      181 cgaggctcgc gggtacgctc gccccaccag cggtggaaaa attgcgcatc gcgggagtgc
      241 gcccaccgtg gggtgtgttg atcatcaatg agtttgtatg tatctccctt cgcattgaag
      301 agcctgttgc tggtggggcg agcgtacccg cgagccgtct gcgcaacgaa gttgctccgc
      361 tgtttccgtt tcctatttcc cgctccaaac caaatcaaaa actctccctt tgaatggcca
      421 atcctcgatt cgttgaacca attgtttgcg taatggattt tcctgcacat aaatccattt
      481 ttccgagtaa tgctgtcctt cgcgcaaacg atgatgccag ccccgcgatt gaaaacgcca
      541 atcactgcgg ccatgtttca ggcgaaactc acgcttccaa aaggaaatcc atgtctcgat
      601 atcaaagtgt aaatctcgtg cggcgcaaaa aacgtgcaga tgatcgggca tcaataaata
      661 atcgcccacc aaccatgcgg acgcttgcga ccaggtttgg cgcagcaatt gatgcgctgt
      721 ttcgttggcg agccacggtt cgcgtttttt ggtggtgacg gtcaggagga cgatgtttga
      781 ttccccaaga tggatgtgaa ctcccggcga aggattttgc ctgttgggtg gttcttgatc
      841 ggaagattgt ttcacggggg tggaaattag cgaacggatt gaatgtttgg aagcttttaa
      901 gaaagatgtc agtcaacgag gctcgcgggt acgctcgccc caccagcagt ggaaaatttg
      961 cgcatcgcgg gagtgccccc aacgtcgggt gtgttgatca tcaatgagtt tgtacgtatc
     1021 tcccttcgca ttgaagagct tgttgctggt ggggcgagcg tacccgcgag ccatctgcgc
     1081 aacgaagttg cgcaaatgct gtcagcgttc ggccttgaca cgtcaagcca tgctaaaaac
     1141 cgaataaaca tctctccatc aatgaattac cgcattttta gccgaaaact agtcaaggct
     1201 ggtcaagcca ttttaatagt tgagggtcga tggttgaggg ttgagtgtgg gaatggcaaa
     1261 agagtgattc gatggctttt cccgccatcc tctatcctcc attctctatt ctcgtcattc
     1321 catgagtgtg ttgcttatgg agtgggcaat gcgggttgcg gattgcggaa tggcgggcgc
     1381 aaatccgtgt tggacggggc gggatggccg ggttacaatt tgcctgttcc ggctggtttt
     1441 ggcattggcc ggattatcga aaacagggct tgcaaagccg gacgtggacg tctaggctct
     1501 ccactctttt ttgagagacg ccggaaacgg cgggcggcaa tgaaaccatg gttcgccgcc
     1561 accaaagcaa ttgttattta tggcagtcaa aattcggatg aagcgggtcg ggacgaaaaa
     1621 cacgccggtg taccgcatcg tggtggccga cggccgcagc ccgcgcgatg gaaagttcat
     1681 cgaggaaatc ggcacttaca cgccgttgaa gaaggacgcg aatttcacgc tcgatctgga
     1741 gcgggcaaat tattgggtga gcaagggcgc gcagccgagc gacacggtcg caagtttcat
     1801 caagaaggcg gcgaaagctg ccgcagccgc gccggccacc gccgcagcat aaatttcgaa
     1861 ccggggcgcg cgatgcaagc gtttttggaa tatgtggtca agggattggt gggccatccc
     1921 gaagacgtga cggtcacgcc ggtggagcgc gaaggcatga cgatttatga attgcgcctg
     1981 aaccccgacg acgtcggcaa ggtgatcggg cggcagggaa tgacgatcaa cgcgatccgg
     2041 tcgttgttgc tggcgggaag cgcgaagaaa gggatgcgct gcagcctcga aatcgtcgag
     2101 gaacagccgg cgcgttagcc gctgccgagg gcatggcagc gaaaggctga aggcttacgt
     2161 tttcagccac ggctcccaag atgaagattg acgtgctcac gttgtttccc gcgatgttcg
     2221 cggggccgct tgatgagagc atcgtccagc gcgcgcgaaa aaatggcgcg ttggatttgg
     2281 cggtgcataa tttgcgtgat tacacgcatg accggcacaa aacggtggat gaccgtccgt
     2341 tcggcggcgg tccgggaatg ttgttgaagc cggaaccgct ctttgaagca gttgaaaatt
     2401 tgggccgcga aaagtcgcga gtgattttgc tttcgccagc agggcggaag ttcgatcagg
     2461 ccatcgcccg cgaactggcg caagcggagc atttgatttt ggtttgcggc agttacgaag
     2521 ggttcgacga gcggatccgc gaaagtttgg cggacgatga attgtcgatc ggcgatttcg
     2581 tgctgacgaa cggcgcgttg cctgccatga tgatcatcga cgcggtgacg cggttgttgc
     2641 ccggagtcct gggcgacgac gaaagttcgc tggacgaatc gttcagcaaa gatttgctgg
     2701 aatatccgca ttacacgcgg ccggcggaat ttcgcgggat gaaagtgccg gacgtgctgc
     2761 tctcgggcaa ccacgcggag atcgcgaaat ggcgggccga acaggcgcgg ttgcgcaccg
     2821 cggaacgcag gccggatttg ttgaaacgga aaacggagtg atggagtggt ggagtaatgg
     2881 gatttcccca gcgctccttc actgcaacac tccagcgcct ttagaattta tgaaccaagc
     2941 attacttgat aaaattgaat cggaacagta tcgcaagaac cccgtcacgt tcgccgtggg
     3001 cgattctgtc aaagtccaca cgaaggtcgt tgaaggcgaa aaggaacgca tccaggtctt
     3061 tgcgggagtg gtcatcggcc gtcgcggtca cgggttgaac gagactttca ccgtccgccg
     3121 catcagctac ggcgaaggcg ttgagcgcgt gttccccatc cattcgccgc gcgtggacaa
     3181 ggtggaagtc gagcgcaaag gctccgtccg ccgcgccaag ctcacttacc tccgcaaacg
     3241 tctcggcaag ggggcgacgt tggtgaagga aaaagaaacc cgtggcgaag ccgcttccaa
     3301 agctccgtcc aaggccaagg ctgctgctgc ggaacccgtc acggcataat caagctggac
     3361 atgatttcaa aggcgaggct tcggcctcgc ttttttgctc tcaccgaagg cggccgtagt
     3421 aaagagtggt gtcgtactca aaagtgactt tgccgtcctt ctggtgttct ttgaaaattt
     3481 gagccaaagc ttcaaccatg ggagcatgtt tgagatggcc tgcttccggc gcatatgatg
     3541 acgacatcaa ccggccttgc aaaccttcaa aattgaacac ttgttcattg ggaaagctgg
     3601 cgagcttgaa accgctgtcg ccaaaaaacg acccaatcat ttgcgcatcg gcatgtttgt
     3661 gccctacctt ctcgtaatca gtcccgtacg tgagcagcag attttcgtag gcctgaaaga
     3721 atgggcgcgc cgagatgttg cgatcgttcc aaatgagcgc caaccaaccg cccggtttga
     3781 ggatgcgcaa aaattcccgg cgagctttct cacgatcaaa ccaatgaaac gcctgcccgg
     3841 ctgtgatcat gtccacgctc gtcatttcaa ggccagtcgc ttcggcagcg gcattgatgc
     3901 tttggaagag cgggtacgaa acaagcaacc gttcggcggc ttcgcgcatc tcacgattcg
     3961 gctcgatgcc gatcacgcga ttgccgtgtt tcaaaaaaag ctcggtcaac tttcccgtgc
     4021 ctgagccgat gtcagcgacg agattcgacc caagaaaccc acattctcgt tcgagaaagg
     4081 ggatgatggc tggcggatag tctggtcgat atctgatata attctcgacg cggctggaga
     4141 agcgctgggt cgggtcttta tttttgcctt cagccttcaa agcaaccttg ggatcaaagc
     4201 atcctggtta gttcttcgat tttccacctg gcagacgccg ctcgatccgc agcaaagtgt
     4261 tttcaacctg cagaatcaac ttttcatgct ggtgttggtt acgctcgctc atttgctgaa
     4321 gatcaaaggt cacgctcgac aacggttgcc aactcatcca cttcgccgcg caattgtttg
     4381 atggcttcct tgttctcccg cgtgtcccgg gcaagaaaca acaattgggt caccagttca
     4441 gccaattgtt tgaacatggc gcaagttttt ccaaatacga tccagcttct ttcgagtgga
     4501 agattgcagc ctccggatct cagcatcatc agtcttattg gcccggtgta tcgctgccaa
     4561 ctccttcaag ccatcgcgga taatgacggc atattcgcca aaatcgccat taaactttga
     4621 agcagttgcg gcctttgttt tcatagcctt attttaatct gaaaaaagat gaaggcaaac
     4681 cttttaaact cagataattc aggtcttttt ttctgaaaaa tgccgcgtcg cttcatcaat
     4741 taaaagcaat ctttcttcgc gtgtctccag gtttttgatg cgcgcgtcgg atgcattctc
     4801 gatttttatt gtaaacgccg cccttaattc ctgcgcgcgc ttgaattgct tgtccggctt
     4861 ggctggcgtg agcgaatatt ccacaacgta tccggcagag cgcaaatctt ggatgagttt
     4921 cagcgattgc ggacgcagcg cttcatcttc aatcaaaacg aacgcgtcca aaccggcatc
     4981 gaactttggc agcaggtcgc gcgctttgag aagttcgagc aacacgacgt cgcccaagcc
     5041 gaagccaagc gcaggcaggt tcactttgcc gccgctgatg agcttgacga gattgtcgta
     5101 acgtccgccg cccgcgatgg cgcggaattc acctttctga tcaaacgctt caaataccac
     5161 tccggtgtaa taagcgagac cgcggatgac ttggtaatcc accttcacga aatcactgag
     5221 gccgcgagcg gcgaggttat tcaaaatggc ttccagttcc gcggtcggtt ttccggtcct
     5281 gatgaacgcg gtgatttcat cgaatgaaaa gccgagcgcg gcgagcttct ttttgctttc
     5341 gtcgggcgct tcgcgttcga gcttgtcaat gatttgatag aactcatacg ccttcgaatc
     5401 atcgggacaa cgctgg
//
LOCUS       DASZMM010000135         6853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3353061, whole genome shotgun sequence.
ACCESSION   DASZMM010000135 DASZMM010000000
VERSION     DASZMM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3353061"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VG754_03370"
     CDS             complement(<1..276)
                     /locus_tag="VG754_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWF18276.1"
                     /translation="MPGSGAVTRQKLGRLTIFFGMTPGVGKTYAMLEAATEQTLAGRD
                     VVIGFVQTHSSKATASLTKKLSPMPGCHATSEQPAVPLDLDALLARKP"
     gene            complement(412..1824)
                     /locus_tag="VG754_03375"
     CDS             complement(412..1824)
                     /locus_tag="VG754_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HWF18277.1"
                     /translation="MISVNPLLMLPFGALLALLALAPLFFPDWWGKHYPKVTLGLALL
                     VVAYYILGLRAGGHVAGVAHEYFSFITLVGSLFVVSGGIHINVKGEATPFSNVIFLLI
                     GALTANVLGTTGASMLLIRPWLRSNKYRVTGHHVVFFIFIVSNVGGCLTPVGDPPLFL
                     GYLMGVPFSWVTAHCLPMWATGLGILLLLFYVVDRRNYIRAPREVRVEQTGHEHWKFD
                     GLGNVGFLGIVLGAVFIDQPPFLRESLMIAAALGSYFTTRKHVHESNHFSLNPIKEVA
                     ILFIGIFATMLPALDWLQENASRLQEATPGMFYWGSGVLSSVLDNAPTYLCFLKAGIG
                     RFVQPEIVTEVARLVQNHGATLASVAGTHAEEIKSTFAALQQFHASDIANGHVSTDQI
                     QVAYLLANLKLNIYIVAVSVGAVFFGANTYIGNGPNFMVKAIADHQKVHTPSFPAYVY
                     RYTLPFMIPMLFIVWLIFFH"
     gene            complement(1925..2254)
                     /locus_tag="VG754_03380"
     CDS             complement(1925..2254)
                     /locus_tag="VG754_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18278.1"
                     /translation="MHPEFNKILTAALGLLAGGVIGLTFGIIQTAAQRRNQRLQESGK
                     LTSGWAVMPGSGKRVAYLLVALALVQFFCPLLFNNGSQWWVSGGVVAGYGWTLYKQLR
                     ERLAQNR"
     gene            complement(2341..4155)
                     /locus_tag="VG754_03385"
     CDS             complement(2341..4155)
                     /locus_tag="VG754_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWF18279.1"
                     /translation="MIKKLINSITVMFLLVLIASPVLADDTNAPASTNAMAAMTNMAP
                     AAPAPGTTPDPGGTATGMAGDAQAAAGVSTFVVTAPTDLTGDDLKDTNKVAAFKDAKK
                     AFDDYTAQSAKEPLALKLADSVGHNRVAINFMWTLVTGFLVMFMQAGFALVETGLCRA
                     KNAGHTMAMNFMIYPMGMLGFYLCGFALMFGGMADPNSPSGGGISTMGGYAGLNHEFG
                     IHIAGKFFGLFGTKGFLLQGAGYDSAAFALFLFQMVFMDTTATIPTGGAAERWKFSAF
                     MIYGCCIGTIMYPFFGNWVWGNGWLAELGVNFPGLGHGHVDFAGSSVVHMQGGVICLI
                     FCWLIGPRYGKYNKEGKIVHPITPHSIPLVMLGTFILAFGWFGFNPGSSLAGTDLRIA
                     VVAVNTMLASATGALGATLWMWWIRTKKPDPSMMCNGMLAGLVAITCPCAFVNAGGAS
                     LLGLIAGILVVESVFFFDKIGIDDCVGAISVHGVNGAWGCLSLGLFADGTYGDGWNGV
                     PGKVTGLFYGGGMGQFWAECIGVITCFVTLSVLSLIVYFIAEKLVGNRVPLDVEIEGL
                     DIPEMGVPGYAGVVMDKQSETPMIPESSSSSKPAMAKT"
     gene            complement(4201..4539)
                     /locus_tag="VG754_03390"
     CDS             complement(4201..4539)
                     /locus_tag="VG754_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWF18280.1"
                     /translation="MKKIEAIIKPFKLEDVKGALAEIGVNGMTVTEVKGFGRQKGHTE
                     TYRGSEYTVDFLPKIKLETVVDNEIADSAVEAIIKSARSGKIGDGKIFVTSLDEVVRI
                     RTGEKGKTAI"
     gene            5744..>6853
                     /locus_tag="VG754_03395"
     CDS             5744..>6853
                     /locus_tag="VG754_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF18281.1"
                     /translation="MISFAFSGVLSAQTLLHRYSFASDASDSVGTANGHIVAPNGGSP
                     ATISNGLNLPGSGGPFGASGYVSLPNGILTNTASLTLECWFTQNSGNTWATPWDFGNN
                     GNQNFALIPYPGNNGNNMEVAFTPNGNERDLQTATAFPNGIEEYVTVTFNSATLVADI
                     YTNATLDAAVTLPNSTYTPGLIGGSLGTTENMLGNDVYGDPQFNGTIYEFRIWNGVVS
                     QRYLAASAIAGPGVLITNLHPTGMQLTAGSGVVLTGTEQAVATVELSQTGTNQFLATS
                     DATNWVSSNPGVLTVNGNGFISGVGLGTATVSATINGVTATSGNIIVTPQILQHRYSF
                     VSDATDSVGGANGTLVPPTTGNPATISNGLVLPGNT"
BASE COUNT         1732 a         1864 c         1801 g         1456 t
ORIGIN      
        1 cggcttccgc gcaagcaggg catccaaatc cagaggcacc gcaggttgct ccgaggttgc
       61 gtgacaaccg ggcattgggg aaagtttttt ggtgagcgaa gcggttgcct tggagctgtg
      121 cgtttgcaca aaaccaatca ctacatcccg cccggccagt gtttgctcag tggccgcctc
      181 cagcatggcg tatgtctttc caaccccggg tgtcatgccg aaaaaaatcg taagtctgcc
      241 cagcttttgt cgggtgactg ctccactgcc aggcattaac agacgtccga gtttcggttt
      301 gcgggcagca atcacacgcc aaatataact aaacgtagcc ccagccgcag cttaattttg
      361 agctgcgcca cagccttcaa cccaacgagg acgcaaccct gtcattttga cctaatgaaa
      421 aaagatcagc cacacgatga acaacatcgg aatcatgaac ggcagcgtgt agcgatagac
      481 atacgccgga aaactgggtg tgtggacttt ttgatgatcg gcgatcgcct tgaccatgaa
      541 gttcgggccg ttgccgatgt aggtgttcgc gccgaagaaa accgcaccca cgctcactgc
      601 gacgatgtag atgttcagct tcaggttggc caaaagataa gcgacctgga tttggtccgt
      661 gctcacatgg ccattggcga tgtcggaggc atgaaattgc tgaagcgcgg caaaagtgga
      721 tttgatttct tcggcatgag ttcctgccac gcttgccagg gtcgcgccat ggttttgaac
      781 gaggcgcgcg acttcagtga caatctccgg ctgcacaaac cggccgatgc cggccttcaa
      841 gaaacagaga taggtcgggg cattgtcgag cacgctggaa agcacgccag agccccaata
      901 gaacattccc ggcgtggctt cctgcaagcg gctggcgttt tcctgcaacc aatccaacgc
      961 gggcagcatc gtcgcaaaaa tgccgatgaa aagaatggcg acttccttga tgggattgag
     1021 cgagaaatga ttggattcgt gcacatgttt gcgcgtggtg aaatacgaac cgagcgcagc
     1081 cgcgatcatc aagctttcgc ggaggaatgg cggctgatcg atgaaaaccg cgccgagaac
     1141 aatgccgaga aaaccgacat tgccaaggcc gtcaaatttc caatgttcat ggcccgtctg
     1201 ttcaacgcgc acttccctcg gcgcgcgaat ataattccgc cggtccacga cgtagaacag
     1261 cagcagcagg atccccaagc ccgttgccca cataggcaga caatgcgcag tgacccatga
     1321 aaacggcact cccatcaggt atccaagaaa cagcggcgga tcgccgactg gagtgaggca
     1381 tccgccgacg tttgagacga tgaagatgaa gaaaacaacg tgatggccgg tgacgcggta
     1441 tttgttgctc cgcagccatg gacggatcag aagcatggac gcgccggtgg tgccgaggac
     1501 attggctgtc aacgcgccga tcagcaggaa aatgacattt gaaaatggcg tggcttcgcc
     1561 tttgacattg atgtgaatgc cgccggagac cacgaacagc gagccgacca gcgtgatgaa
     1621 actgaaatat tcatgcgcga caccagcgac atgccctccg gcacgaaggc caagaatgta
     1681 ataagccacc acgagcaacg ccagtcccaa ggtgactttc ggataatgtt ttccccacca
     1741 atcaggaaaa aacagcggcg caagcgccag cagcgcaagc aacgcgccga acgggagcat
     1801 cagcaatgga ttgacagaaa tcatgtcacg atgctaacag aatgaaaaaa gcggcgtggc
     1861 cggaaagacc acgccgtgac cagtcgtgcg ggaatatatc tgtaaaaccg cacgccaaga
     1921 tttttcaacg attttgcgcc agacgctcgc ggagttgttt gtaaagcgtc cagccgtatc
     1981 cggcgaccac gccgccggaa acccaccatt gggaaccatt attgaaaagc agaggacaga
     2041 agaattgaac gagggcaaga gccaccaaca agtaggccac acgcttgccg gagccgggca
     2101 tgaccgccca gccactggtc aatttgccgc tctcttggag ccgttgattg cggcgttgcg
     2161 cggcggtttg gataatgccg aaagtcaatc caatgactcc acccgcgaga aggcccaagg
     2221 ccgctgtcag gattttatta aattcaggat gcatatattt tttgggatta ctggtcttat
     2281 attttcttgg tttataagct gcgcggggag gtttgctccc cgcgcagtgc aatttgatta
     2341 tcaggtcttg gccattgcgg gcttgctgga agaagacgac tccggaatca tcggggtttc
     2401 agactgcttg tccattacca cgccagcgta acccggcaca cccatttccg gaatatccag
     2461 accttcgatc tcgacatcca gcgggacacg gttgccaacg agtttctccg cgatgaagta
     2521 aacgatgagc gacaagactg acaatgtaac gaagcaggtt atcactccga tgcactccgc
     2581 ccagaattgg cccatgccac cgccgtagaa gaggccggtc accttgcccg ggacaccgtt
     2641 ccagccgtca ccataagtgc cgtccgcgaa taggccgagt gacaaacagc cccaagcgcc
     2701 attgacgcca tggaccgaaa tggcacccac gcaatcgtca atgccgattt tgtcgaagaa
     2761 gaacacggac tcgactacca agatgcccgc tatcaagccg aggagactgg ctccgcctgc
     2821 gttaacgaac gcgcagggac aggtgatggc gaccaagccg gccaacatgc cgttgcacat
     2881 catcgaggga tccggcttct tggtgcgaat ccaccacatc caaagtgtcg caccgagagc
     2941 gccagtggca gaggcaagca tcgtattgac cgcaacaacc gcgatgcgga ggtcagtgcc
     3001 cgcgaggctg gagccagggt taaacccgaa ccaaccgaag gcaaggatga acgtgccaag
     3061 catcaccaat ggaatactat gcggcgtgat cggatgaaca attttgcctt ccttgttgta
     3121 tttgccgtaa cgcggtccaa tcagccagca gaagatgagg caaatgacac caccttgcat
     3181 gtgcacaacc gaggaaccgg cgaagtccac gtgaccatgg ccaagaccgg ggaagttcac
     3241 gccgagttcc gcgagccagc cgttgcccca cacccagttg ccgaagaacg gatacatgat
     3301 ggtgccgatg cagcagccgt aaatcatgaa cgccgagaat ttccaacgtt cagccgcgcc
     3361 gccggtcgga atcgtcgccg tcgtgtccat gaacaccatc tgaaacagga acaacgcgaa
     3421 ggccgccgaa tcatatccag cgccttgcag caggaagccc ttcgttccga aaagaccgaa
     3481 gaattttccg gcgatgtgga tgccgaattc gtgattcaat cctgcgtatc cacccatcgt
     3541 gctgatgccg ccgccgctgg gggaattggg atccgccatg ccgccgaaca tcaacgcaaa
     3601 tccgcacaga taaaatccga gcatgcccat cggataaatc atgaagttca tcgccatggt
     3661 atggcctgcg tttttcgctc ggcaaagacc ggtttcaacc aatgcgaaac ctgcttgcat
     3721 gaacatgacc aggaagcccg tcaccaacgt ccacatgaag ttgatcgcga cgcggttgtg
     3781 accaacggag tcggccaatt tcaacgcgag cggctccttg gcagattgcg ccgtataatc
     3841 atcaaacgcc ttcttcgcgt ccttgaacgc agccactttg ttggtgtcct tcaaatcatc
     3901 accggtcaaa tccgttggcg cggtcacgac gaaggtgctg actccagccg ccgcttgagc
     3961 atcgccagcc atgccagtgg cagtgccgcc cgggtcgggg gttgtaccgg gtgctggtgc
     4021 ggcgggcgcc atgtttgtca tcgcggccat ggcattggtt gaagcgggcg cgttcgtgtc
     4081 gtccgccagc acgggcgaag cgatcagaac gagcaggaac attactgtga tcgaattgat
     4141 tagttttttt atcatatatg gtttggctga ggcgttgtta gattccgggg ctggggtaac
     4201 tcaaatggcg gtttttcctt tctcgcctgt gcggatgcgg acgacttcat ccaggctggt
     4261 gacgaatatc ttgccgtccc cgatttttcc gctacgggcc gacttgatga tggcctcgac
     4321 cgctgaatca gcaatctcat tatccacgac ggtctcgagc ttgattttcg gcagaaaatc
     4381 cacggtgtat tcactgccgc gataagtttc agtgtggcct ttttggcggc cgaatccctt
     4441 gacttcggtg acggtcattc cattgacgcc gatttccgcg agggctccct ttacatcttc
     4501 gagtttgaac ggctttatga tggcttcgat tttcttcata gttatttggt tgacggttcg
     4561 gaaactgatg atggtttaag gacccggtcg atacatttct gcgtgcccat gcatggacga
     4621 cggaataccg attcggcctg atgatgattt gaacacaaaa gtgcctcaag cacgcccaat
     4681 gccagcaacg aaataatttt ttacgattat ttaatacact gatttacacc gaattagatg
     4741 ccggcctccc tgggaattgt ctttagagcg ctgaaaaaga atgtattgat ttagtcatca
     4801 tggtggaatc gtttaaaaaa gccctgactg cgcttcgctt ttttgcgtca aatattaatt
     4861 aaatattaaa aaatcgcctt tcatatgaaa atgaaaagag tttctgatgg tgtgcaaagg
     4921 gagtaaaatg ccttttaatt accggcagag tttttaaaac taaaaaacga actgaaaggt
     4981 ttttccgctg ttaaatactt attaatgatt tattagaatt tacggtgggg tagagtcatt
     5041 ttagttggga aacatacaaa aagccggatt ctccaaagaa atcaggccat actatctggc
     5101 gttttttgcc cacccatata tctggaaaaa ctggttgctg aatggggatt gtatttgctc
     5161 catggtccga ctttgatgcg ctcgctgctg cgacccaagc caaaaagcaa atcacgatac
     5221 aatggagaca accaacctaa agagcgacgc attaactcgg caaccaaata aatgagtcat
     5281 ctgcgtaatt tgctttttta caggttcagc cagcgaagct gcagagcatc tcacctgaca
     5341 actgggagat tttcgcttca cttttacgat gtgggctgtg ccagtaaaat cagccgtcga
     5401 tgaacaatcc aaacccgctt gaacaggctc aaattgcggg agaacaacgt ggctgaagcc
     5461 gaaaaaagtg atgtggccac tttatgccac atgacggaaa gcacagtttt gttagccttt
     5521 tcgaaagccc gaacaatgcg cgtcttggaa ccaatgtcgt ctattggcgt ctctacgaga
     5581 cattgtgcgg gttctgggcg gcactcggaa taagcgccga cacgatgcaa tcatgaccaa
     5641 caatactgaa caactaatcg caaaagatat gaaagaatcc acgagaacaa cggactggcg
     5701 ggatggatca acccgcaccg tggtccagtg catgtttgtg gcaatgattt cctttgcctt
     5761 ctccggcgtg ctctcagcac aaacacttct gcatcgctac agtttcgcga gcgacgcatc
     5821 ggactccgtt ggcacggcca atggccatat tgtcgcgccc aacggcggca gccctgccac
     5881 catcagcaac ggtcttaatc tgccgggcag cggcggccca ttcggcgcat caggctatgt
     5941 atctctgccc aacgggatct tgaccaacac cgcctccctg acgctcgaat gctggttcac
     6001 gcaaaactca ggaaatactt gggcgacccc ttgggatttt ggcaacaacg gcaaccagaa
     6061 ttttgcgctg attccctacc caggcaacaa cggcaacaac atggaagtgg ctttcacgcc
     6121 caatggaaac gagcgcgatt tgcaaacggc gactgcattc cccaatggca ttgaagaata
     6181 cgtcaccgtg accttcaaca gcgccacact ggtggctgat atttacacca atgcaaccct
     6241 tgatgcagcg gtgactttac ccaactcgac ttacacgccg ggcctcatcg gaggcagcct
     6301 tggcacgacg gaaaacatgt tgggaaatga cgtctatggc gacccgcagt tcaacggcac
     6361 catctatgaa tttcgcatct ggaatggcgt cgtttcacaa cggtatcttg ccgcgagcgc
     6421 aattgcaggt ccaggcgttc tgatcaccaa cctgcatccc acggggatgc aacttacggc
     6481 aggttcaggc gtcgtcctta ccgggactga acaggcagtc gccacagtgg agctgtcgca
     6541 aacaggcaca aaccaattct tggcgacgag cgatgcgacc aattgggtca gcagcaatcc
     6601 gggtgtgctc acggtcaatg ggaatggctt tatcagcggc gttggcttgg gcactgccac
     6661 agtgagcgcc acaatcaacg gcgttaccgc aaccagcggc aacatcatag tcacgcctca
     6721 aatccttcag catcgctaca gtttcgtcag cgatgccacc gattcagtcg gcggcgcgaa
     6781 cggaactctc gttccgccca caaccggcaa cccggcgacc ataagcaacg gcctcgtcct
     6841 gcccggaaat acg
//
LOCUS       DASZMM010000136         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_602633, whole genome shotgun sequence.
ACCESSION   DASZMM010000136 DASZMM010000000
VERSION     DASZMM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602633"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /locus_tag="VG754_03400"
     CDS             <1..1656
                     /locus_tag="VG754_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18282.1"
                     /translation="MAPSNPVQERRSESAPLEADEAKRRARFLKAAFCVWVILLAIYQ
                     FSENTADPDLWGHVTFGQEILHSHAIPKTEIYSWTSAGQPWINHELLAEIFLGGAHAM
                     LGGSGLLLLKMAVGLLTFALCLRMALADMKEGEQWIAWGFAALAVVEISFGFSARPQI
                     FTALCLTLELMLLRRIHAGAPLWALALPPLFIFWINTHGGALAGVGLLAVTAAATTGQ
                     FLFSKKFKRDANEQTPTLKIVFALWLALVGVIGALFCNPWGGGLVRFLIESVSWLRPE
                     IQEWNPTPVNWDHAAMFMLMALGVFAWAFTRRRRAWWEAAVCAAFAVLALRSVRNVPL
                     FALVALALVPRQLADALARFRRFFARIEMLWQRADIQNFAAIMLVLTGVGIGAATFTL
                     HKEHPFTMEAPRAEYPVSAISFIQEHGLRGKLFVFFDWGEMTIFHLPDCPPSIDPRLD
                     TCYSRALIAAHWKFYNGEPCDEKVLDPFAADLALLPVKLAGALTLAHHPGWKAVYYDE
                     TAVLLVRDVTRFPGLQGLALPVEGAKTASLGRAAFADRSPRWK"
     gene            1972..>2429
                     /locus_tag="VG754_03405"
     CDS             1972..>2429
                     /locus_tag="VG754_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HWF18283.1"
                     /translation="MKLVTIFLALLMAQIVSAQTKTSLYDIPLKDIDGKDTSLKAYKG
                     KVLLIVNVASKCGFTPQYEGLEALQKKFEKQGFTVLGFPCNDFMGQEPGSPDEIKQFC
                     SSKYSVTFPLFSKDHVNGKEAQPLYQQLTGNDSPFPGRITWNFNKFLISR"
BASE COUNT          517 a          632 c          692 g          588 t
ORIGIN      
        1 atggctccaa gcaatccggt ccaggaacga cgcagtgagt ccgcgcccct cgaagcggac
       61 gaagcaaaac gccgcgcgcg atttttgaag gccgcatttt gtgtttgggt catcctgctc
      121 gcaatctacc aattttccga aaacaccgcc gatccggatt tgtggggcca tgtgactttc
      181 ggccaggaaa ttctgcacag ccatgcaatt ccaaagactg aaatctattc atggacctct
      241 gccggtcaac catggatcaa tcacgaattg ttggcggaaa tttttctcgg cggcgcccat
      301 gcaatgctcg gcggaagcgg acttctgctc ttgaaaatgg ccgtcgggtt attgaccttc
      361 gcgctttgct tgaggatggc gctggcggat atgaaggaag gcgaacaatg gattgcatgg
      421 ggattcgccg ccttggccgt ggtggaaatc tcgtttggct tttcggcgcg cccgcaaatt
      481 ttcacggcgc tttgcctgac attggaatta atgctcttgc ggcgaatcca cgcaggagcg
      541 ccgctctggg cgctggcatt gccgccgctg tttatttttt ggatcaatac acatggaggc
      601 gcgttggccg gtgtgggatt gctcgctgtg accgctgccg cgacgacggg acagtttttg
      661 ttctcgaaaa aatttaagcg cgacgcaaac gagcagacac cgacgcttaa aatcgttttc
      721 gcattatggc tggccttagt cggtgtcatc ggcgcgttgt tttgcaatcc atggggcggg
      781 ggattggtcc gttttttgat cgaaagcgta agttggttgc gaccggaaat tcaagaatgg
      841 aacccgacgc ccgtgaattg ggaccacgcg gcgatgttca tgctgatggc attgggagta
      901 tttgcgtggg cgttcacgcg gcgacggcgc gcatggtggg aagcagcggt gtgcgcggca
      961 ttcgcggtgc tggcgttgcg gtcggtgcga aatgtgccgt tgtttgcgct ggtggcgctg
     1021 gccttggtgc cgcgacaact ggcggatgcg ctggcaagat ttcggcgctt ttttgcgcgc
     1081 atcgaaatgc tttggcaacg ggcggacata caaaattttg ccgcaatcat gctggtcttg
     1141 actggcgtcg gcatcggcgc ggcaacgttt acgttgcaca aggagcatcc gttcaccatg
     1201 gaagcgcccc gcgccgaata tccagtttcg gcgatttcat tcatccaaga gcatggcttg
     1261 cgcggaaagc tgtttgtttt tttcgattgg ggtgagatga caatttttca cctgccggat
     1321 tgtccgccgt ccatcgaccc acggctcgac acctgttact cgcgcgcgtt gatcgccgcg
     1381 cattggaaat tttacaacgg cgaaccgtgt gacgaaaaag ttttggatcc gtttgccgcg
     1441 gacttggcat tgctgccggt gaaactggcg ggcgcattga cccttgcgca tcatccgggc
     1501 tggaaggcag tctattatga cgaaaccgcc gtattgctgg tgcgcgacgt aacgcgtttt
     1561 ccgggattgc aggggcttgc gttgccagtt gaaggcgcta aaaccgccag cctcggccgc
     1621 gccgcttttg ccgaccggag tccgcgctgg aaataagagg aacttcattt aaccacagat
     1681 aaacacagag gaacgcggat aagaatgaat tgcggttctc gcaaacttcg cgatgcatcc
     1741 atttcctggc aacgatggta ttgtttttct cccatctgcg ttaatctgtg ttcatctgtg
     1801 gttaaactct tttcaaaaat aaaaaaaccc ccggtgtcct tggtcccggg gaaaaattta
     1861 gggtgcggaa aattgatgcc aaaaaatgcg gctttgcgga atagggtgaa ctccccatca
     1921 tggcgatggc gaggcggttg actttttggc agtctgttct attgttcgcg catgaaacta
     1981 gtcaccattt tcctcgcatt gcttatggcc caaatcgttt cggcccagac caagacttcg
     2041 ctctacgaca tcccgctcaa ggacatcgac ggcaaggaca cctcgctcaa ggcatacaag
     2101 ggcaaggtgt tgctcatcgt caatgtggct tccaaatgcg gtttcacgcc gcagtatgaa
     2161 ggcctcgagg cgctgcaaaa gaaattcgaa aaacagggct tcacggtgct gggatttccc
     2221 tgcaacgatt tcatgggtca ggagcccggc tcgccggacg aaatcaaaca attctgctcc
     2281 agcaagtaca gcgtgacttt ccccctgttt tccaaggacc acgtcaacgg caaggaagcg
     2341 caaccgctct accagcaatt gaccggcaac gactcgccgt ttcccggcag gatcacctgg
     2401 aacttcaaca aatttctcat cagccgcga
//
LOCUS       DASZMM010000137         6898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3353701, whole genome shotgun sequence.
ACCESSION   DASZMM010000137 DASZMM010000000
VERSION     DASZMM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3353701"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VG754_03410"
     CDS             complement(<1..297)
                     /locus_tag="VG754_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWF18284.1"
                     /translation="MSRLKRGLRATLTGMVVNAFLAAGKLAAGLLGHSHALVADAVES
                     FADIFSALVVWRGLVVAAEPPDQEHPYGHGKAEAIAAAMVSTMLLLAAIGITITS"
     gene            complement(535..1749)
                     /locus_tag="VG754_03415"
     CDS             complement(535..1749)
                     /locus_tag="VG754_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HWF18285.1"
                     /translation="MATIKPFAALRPRPELAAKICELPYDVMSSAEARAMAAGNPLSF
                     LHVSKPEIDLPPDTDVYSPAVYAKGKENFAKLIADGSLRQDAAPCFYLYRQIMGKHSQ
                     VGLVAAASCEDYLRNIIKKHEFTRPDKEDDRVRHIETLNSQTGPVFLTYRANAALDEL
                     FKKKSAETPDIDFTAKDGVRHTAWVIGDAQTIQRIESEFARIPFLYIADGHHRSAAAA
                     RVYQSRKGAGQSAYFLSVIFPHDQMQILPYNRVLKDLNGLTSAQLLEKLDAIFIIKPN
                     GTPQPSRKHELGLYLDGQWHTLHFRPQFAATSDPIEKLDVTLLQKYVLAPIFGIDDPR
                     TSKRINFVGGIRGTGELEKLVNDGEYACAFSMFPTSIEDLMSIADAGGIMPPKSTWFE
                     PKLRDAMFCHMI"
     gene            complement(1822..3027)
                     /locus_tag="VG754_03420"
     CDS             complement(1822..3027)
                     /locus_tag="VG754_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF18286.1"
                     /translation="MKAVLPLFVFVDACGWEIIKNGPFARSFAPHRRRLDSVFGYSSA
                     CVPSILSGRWPDEHRNWCYFVYDPAHSPFKILRPLRWLPKSVTSRRIFRRWLTKLVKA
                     HLGFRGYFDLYNIPFRQISLFNFTEKKSPLKPGGMNRGPNIFDFLTERNIRHHVSAPE
                     ASETENLAALISDVESETIDFAFLYWAGLDGLLHRVGNQSPQIGDKLRLYEEWIEQLL
                     AAAHEHYAEVRLYVFSDHGMANCDELLDLRAKIDKLPLRVGSDYSVVYDSTMARFWFF
                     NDHARQLIAQALQKIPQGRIIPDAELAEMRTLFTDRYFGELIFLVKEGVLIVPSHMGE
                     RPIRGMHGYHPTDKHSYASLMTNHTDVPDEITAIPHIFQLMTRDAEMAKLRNATPDFE
                     PHLLRNAAH"
     gene            complement(3363..3809)
                     /locus_tag="VG754_03425"
     CDS             complement(3363..3809)
                     /locus_tag="VG754_03425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18287.1"
                     /translation="MHGHIIARTHPAMLKISFPCDLNEVREAVAGVHDFLAKEGWSAD
                     DLMSFDLALVEACNNAIQHAPGQKRSQPIVLETMSDPKQVEFRVHDHTPGFDWPQKIE
                     LPPPESENGRGLYLIRTLMDDTGYLRGQDENILVMHRARAKNSLNC"
     gene            complement(3813..4115)
                     /locus_tag="VG754_03430"
     CDS             complement(3813..4115)
                     /locus_tag="VG754_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWF18288.1"
                     /translation="MKMQVNGETLSISGVKELGVANSDEFRNQARAAMTPEQKNIDID
                     LSEMSHLDSCGLGTLISLRKTTSTRKGTVRLLNPKPGVQQILELTRMHHVFEIVKP"
     gene            complement(4227..4865)
                     /locus_tag="VG754_03435"
     CDS             complement(4227..4865)
                     /locus_tag="VG754_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HWF18289.1"
                     /translation="MNRARKKSPRIPYLVAIVGGSASGKSWLTDQLQNLLGDKAARLS
                     LDDFYRDRSHLPPGRRARINYDHPRAIDWQSVERVLQDCLAGRVARVPSYDFATHSRG
                     STSQSLRPKPVILMEGLWLLRRRSLRRIFNLSIYIDCPAKTRVARRMARDVALRGRTR
                     ESVRRQFMENVEPMHAVHVVPQARWADICLRTPIQKKDLLQLVEKLSKAGCI"
     gene            complement(5030..6178)
                     /locus_tag="VG754_03440"
     CDS             complement(5030..6178)
                     /locus_tag="VG754_03440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF18290.1"
                     /translation="MTASELKQRGHQIAILHGKSTGKSESTWRETFSDCFDLTNGNAS
                     NTAASAIFQFQPDAVYVHKMADLAVIKALVQSGCPLVRMVHDHDLYCMRSYKYNPLTR
                     NICTRATSPYCIFPCGAVLARKRDSGMPFKFVSYRAKQRELAFNRKFHRMIVATEYMR
                     NELVRNEFDPAKIEIHAPAPPAIENNQHSSFSPRNLILYVGQIIRGKGVDVLLESLAE
                     VRAPFECIILGQGSARAQCEALSRDLGLNRRVHFKGYVPPEELQKHLCEASVAVMSSV
                     WPEPFGAAGLEAMRQGLPVVAFDAGGIREWLVDGQTGFLVPWMDRAAFAARIEELLRD
                     KKLARRLGEQARQLAREKYDFANYISGLETMFGRVMTESGKTVVTTPT"
     gene            6392..>6898
                     /locus_tag="VG754_03445"
     CDS             6392..>6898
                     /locus_tag="VG754_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18291.1"
                     /translation="MKRLLILIGSVALVAGCHTNKNNQNTGGMGNTGGMGGGTEYQNT
                     TNYNSGGNTNSSQLHHNASSALVQAGGAGGAGGAGAGGSGGAGTGSGTAGAGPGTGSG
                     TGTTPGAGAGNTGTGGTGTGGTGTSGTGGTGTGTGGTGNGIGTGGGGPNGPNNNNNLS
                     TNKNVFPGP"
BASE COUNT         1595 a         1852 c         1908 g         1543 t
ORIGIN      
        1 ggaagtgatg gtgatgccga tcgccgcaag caggagcatg gttgaaacca tcgcggcggc
       61 gatggcctca gccttgccat ggccgtaggg atgttcctgg tcgggtggct cggcggcgac
      121 gaccaatccg cgccagacaa cgagcgcgct gaaaatgtcg gcgaaggatt ccaccgcgtc
      181 cgcgacaagc gcgtgcgaat ggccgagcaa acccgcggcc agcttgcccg ccgcgagaaa
      241 ggcgttcacg accatgcccg tgagagtcgc ccgcaacccg cgttttaacc ggctcatcgc
      301 cataaccaat cagtgtaatc tagaaacaga agcacggaaa agcctcgttg caaatgctaa
      361 atttagatgg ttcccaggtc tttttcgccg attccaaaga ggacagagaa acaccaaaca
      421 tccaacatcg aacgccgaac atcgaattgt tccaagcatt tagcgttcga tgttcgttgt
      481 tggatgttca gcgttgtctt cgcatttccg tgtccatccg tggccaaaat tattctaaat
      541 catgtggcaa aacattgcgt cgcgcagctt gggctcgaac catgtgctct tgggcggcat
      601 gatgccgccc gcgtcggcga tgctcatcaa gtcttcgatg ctcgtcggga acatcgagaa
      661 agcgcacgcg tattcgccgt cattcaccaa tttttccagc tcgcctgtgc cgcgaatgcc
      721 gccgacgaaa ttgatgcgct tgctcgtgcg cggatcgtca atgccgaaaa tcggcgcgag
      781 cacatatttt tgcagcaggg tgacgtcaag cttctcaatc gggtcgctgg tcgcggcgaa
      841 ttgcggacga aaatggagcg tgtgccattg gccgtccagg taaagcccaa gttcatgttt
      901 gcgcgaaggt tggggcgtgc cattgggctt aatgatgaat atggcatcaa gtttttcgag
      961 caactgcgcc gaggtgagac cgttgaggtc cttcaacacg cggttgtaag gcagaatctg
     1021 catctggtcg tgtggaaaaa tgacgctgag aaaatacgcg ctctgtcccg cgcccttgcg
     1081 actctgataa acccgcgccg ccgccgcgct gcgatgatgc ccgtccgcga tgtataagaa
     1141 tggaatacgg gcgaactcgc tttcgatccg ttgaatcgtt tgcgcatcgc caatcaccca
     1201 tgcggtatgg cgcacgccgt ccttggcggt gaaatcaata tccggcgttt cggcggattt
     1261 ctttttgaaa agttcgtcga gcgccgcgtt tgcgcgatag gtcagaaaaa ccggccccgt
     1321 ctgcgaattg agcgtctcga tgtggcgcac gcggtcatct tccttgtcgg gccgcgtgaa
     1381 ctcatgcttc ttgatgatgt tgcgcagata atcttcgcaa ctcgctgctg cgacgagccc
     1441 cacctggctg tgcttgccca tgatttgacg gtagagataa aaacaaggcg cggcatcctg
     1501 tcgcaacgag ccgtccgcga tgagcttcgc aaaattttcc tttcccttgg cataaaccgc
     1561 cggcgaataa acatcggtgt cgggaggcaa atcaatttcg ggtttgctga catggagaaa
     1621 actcagcgga ttgcctgccg ccatcgcgcg tgcctcggct gaggacatga catcgtaagg
     1681 cagctcgcaa attttcgcag ccagttccgg acgcggccgc aacgccgcaa aaggttttat
     1741 cgtcgccata aaaaaggtga gcgaagataa cgcaatgcga cgggaacgca cgaaaaaaaa
     1801 ctttccgatt cgtttggatt tttaatgcgc cgcgtttcgg aggagatgcg gctcgaaatc
     1861 aggtgtggcg tttcgcagct tcgccatctc agcatcgcgg gtcatcaatt gaaagatgtg
     1921 cgggatggcg gtgatttcgt cggggacatc cgtgtgattg gtcatcaatg acgcgtagct
     1981 gtgtttgtcc gtcggatgat acccgtgcat gccgcgaatc gggcgttcac ccatgtggct
     2041 gggcacaatt aacacgcctt ctttgacgag aaaaatcaat tcgccgaaat aacggtcggt
     2101 gaagagtgtg cgcatctccg ccaattctgc gtcgggaatg attcgtcctt gcggaatttt
     2161 ttgcagcgct tgagcaatga gttgtcgcgc atgatcgttg aaaaaccaga agcgcgccat
     2221 ggtcgaatca tacaccactg aatagtcact acccacgcgc aatggaagct tgtcaatttt
     2281 cgcgcgcaaa tcgagcaact catcgcaatt ggccatcccg tgatcgctga aaacataaag
     2341 ccgtacctcg gcgtaatgtt catgcgccgc cgcaagcaat tgttcaatcc attcctcgta
     2401 caaacgcagc ttgtccccga tttgcggcga ttgattgccc acgcgatgca gcaaaccgtc
     2461 cagcccggcc caatatagaa acgcgaaatc aatcgtctcg ctttcgacgt cgctgatgag
     2521 cgcggccaga ttttcggttt cgctcgcttc cggcgcgctg acgtgatggc ggatgtttcg
     2581 ttccgtgaga aaatcaaaaa tgttcggccc gcgattcatg ccgccgggtt tgagtggact
     2641 ttttttctcg gtgaaattga acaaagaaat ttgccggaag gggatgttgt aaaggtcgaa
     2701 atagccgcga aagccgagat gcgccttgac gagtttcgtc aaccaacggc ggaaaatgcg
     2761 gcggctggtg actgattttg gcagccagcg caatggcctc aagattttga aaggcgaatg
     2821 cgccgggtca taaacgaaat agcaccagtt gcgatgctcg tcgggccagc gtccggaaag
     2881 gatggagggc acgcacgcgg aactatagcc aaaaaccgaa tcaagccggc ggcgatgggg
     2941 cgcgaacgag cgcgcgaaag gaccgttctt gatgatttcc cacccgcagg catctacaaa
     3001 cacgaacaac ggcaatacag ctttcacgca aacattgtga ccgattttgg cggcttgtca
     3061 ggtaggtggt gactccatgc cgactccaat gagactcgtt acaaggttac aaagttacat
     3121 ggcgccagcg catcgggagg cttgtgacca tgtaacgatg taaccatttc agagcctgcc
     3181 cgtgggaaaa tagaattagg aaagcaggaa aagacgatgt ttcctcaaga attttgttcc
     3241 tgatttcatg atttcccagt taaaaactct taatctcatt ttctgccgtt catctggatt
     3301 tgccaataaa atagcaggtc ccgactggct tgacgcggac tatgggatgc tactgtaacc
     3361 atttaacaat ttaacgaatt ctttgcccgc gcacggtgca tgactaaaat attttcgtcc
     3421 tgaccgcgca aataccccgt gtcatccatc agtgttcgga tgagataaag gccgcggccg
     3481 ttttcgcttt cgggcggcgg caattcaatt ttttgcggcc aatcaaaacc cggcgtgtga
     3541 tcgtgaacgc ggaattcgac ctgcttcggg tcagacatcg tttccaagac gatgggctgg
     3601 ctgcgctttt ggcccggggc gtgttggatg gcgttgttgc aagcctccac caaggccaga
     3661 tcgaacgaca tcaaatcatc cgcgctccag ccttccttcg ccaaaaaatc gtggacgccg
     3721 gccaccgcct cgcgcacttc attcaggtcg catggaaagg aaattttcag cattgccgga
     3781 tgggtgcgcg cgatgatgtg gccgtgcatg tatcaaggtt tcacaatttc aaaaacgtgg
     3841 tgcatccggg tcaattcgag gatctgttga acacccggct tgggattgag caggcggacg
     3901 gtgcctttgc gggtggatgt ggtcttgcgc agagaaatga gcgttcccaa gccgcaactg
     3961 tccagatggc tcatttctga aaggtcgatg tctatatttt tttgttcggg cgtcatcgcg
     4021 gcgcgggcct ggttgcgaaa ttcatccgag ttggcgacgc ccagttcctt gacgcctgaa
     4081 atgctcaatg tttccccgtt cacttgcatc ttcataaatc aatttttata aatgcctgcc
     4141 tttattccac tggcgctttg cgcagcggtt ggtggtcggt tcgttgctcg aggacagcat
     4201 aaaagaagca caggttgcca catttttcaa atgcaaccgg ctttgcttaa cttttcaacg
     4261 agttgcagca aatccttttt ttggattggt gtgcgcaaac aaatgtccgc ccagcgtgcc
     4321 tgtggaacga catgcaccgc gtgcattggc tcgacattct ccatgaattg ccgccgcacc
     4381 gattcgcgtg tccgcccgcg caaggccaca tcacgcgcca tgcgccgggc cacgcgagtc
     4441 tttgccgggc aatcaatgta gatgctcaaa ttaaaaattc gccgcaacga gcgccgccgc
     4501 agcagccaca agccttccat caaaatcacc ggtttggggc gcaatgattg agaagtggaa
     4561 ccccgcgaat gcgtggcgaa atcgtagctt gggacccgcg ccacacgtcc ggcgaggcaa
     4621 tcttgcagaa cacgttccac actttgccag tcaattgcgc ggggatgatc gtaattgatg
     4681 cgcgcgcggc gtccgggcgg cagatgggag cggtcgcggt aaaaatcatc cagcgacaac
     4741 cgtgcggcct tgtcgcccaa aagattttgc aactggtcgg tgagccagct tttgcccgac
     4801 gcgctgccgc cgacaatcgc caccaagtat ggaattctcg gggatttttt acgggcacgg
     4861 ttcattcagt tttgcacggt tgcggaccga ttgcttcagg cgactggaaa gtcgcctgaa
     4921 cccgcaggct ggaaagcctg cgctacgtag cgcaaacatt cttgtttgcg ggtattgggg
     4981 actttccagt ccccaacctg aaatgatctt ttgaatttca gaaccatggc tacgtgggcg
     5041 tcgttacaac agttttaccg gattccgtca ttacgcgacc gaacatggtt tccagcccgg
     5101 aaatataatt ggcgaaatca tatttttcgc gggccaattg acgggcttgt tcgcccagcc
     5161 gccgcgccaa ttttttgtcg cgcaataatt cttcaatccg ggcggcgaac gcggcgcggt
     5221 ccatccacgg taccaaaaaa ccggtctgtc cgtccaccag ccactcgcgg atgccgcccg
     5281 cgtcgaaggc gaccaccggc aagccctggc gcatcgcttc caatcccgcc gctccaaacg
     5341 gttccggcca gaccgaactc atcaccgcaa ccgaggcctc gcacaaatgc ttttgcaact
     5401 cctccggcgg gacgtaaccc ttgaaatgaa cgcgtcggtt gaggcccaaa tcacggctca
     5461 atgcctcaca ttgcgcgcgg gccgaacctt ggccgagaat gatgcattca aaaggggcgc
     5521 gcacctctgc caacgattcc aacagcacat ccacaccttt gccgcggatg atttggccga
     5581 cgtacaggat gaggttgcgc gggctgaagg aactgtgttg attattttca atcgctggtg
     5641 gcgccggggc atggatttcg attttggccg gatcaaattc atttctgacc agttcgttgc
     5701 gcatgtattc cgtcgcgaca atcatgcgat gaaatttccg attgaacgcg agttcccttt
     5761 gcttggccct gtaactgacg aatttgaatg gcatcccgct gtcgcgcttt cgcgcgagca
     5821 ctgcgccgca cggaaaaatg caataaggcg aagtggcgcg cgtgcaaata tttcgcgtca
     5881 gcggattata tttatagctt ctcatgcaat aaaggtcatg gtcgtggacc atccgaacca
     5941 gcggacaccc ggactgaacc agcgctttta tcaccgccaa atccgccatt ttatggacgt
     6001 aaacagcgtc cggttgaaat tgaaaaatgg ccgaggccgc ggtattggac gcattgccgt
     6061 tagtcaggtc gaagcaatcc gagaaagttt cgcgccaggt cgattcgctt ttgccggtgg
     6121 atttgccatg aagaatcgca atttgatgcc cgcgctgttt gagttcgctt gcagtcaaaa
     6181 ataaattccc ctccgcgccg gcaaaagccc caaagcattc atggacgaag agcagcttca
     6241 ttctcctttg tgattgccaa ataatacagc catttgcccc aattctccac ttttaaatca
     6301 tcggggtgaa tcgggtttta ccctaattga ggagcgcaat gttcaaaagg agtttgtgac
     6361 gaggaacggc gaaataaaac caaacatcat tatgaaaaga cttcttatat tgatcggctc
     6421 ggtggcgttg gtggcgggtt gccacaccaa taaaaacaac caaaacaccg gcggcatggg
     6481 caataccggc ggcatgggcg gcggcacgga ataccagaac actacgaatt acaattcggg
     6541 cggaaacacc aattcaagcc agcttcatca taacgcgagt tccgccctgg tccaagcggg
     6601 tggtgcgggc ggagccggtg gagcaggggc tggtggcagc ggcggcgcgg gaacgggatc
     6661 gggcactgcc ggcgcgggac ccggcacagg gagtggaacc ggtacgacgc ctggtgccgg
     6721 cgcgggtaat actggaactg gcgggacagg cacaggtggg actggaacaa gcgggacagg
     6781 cggaacgggc actggcacgg gcggtaccgg taacggaatc ggaaccggcg gcggcggacc
     6841 gaatggcccg aacaacaaca ataatctctc caccaataag aatgtgttcc ccggccct
//
LOCUS       DASZMM010000138         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1148009, whole genome shotgun sequence.
ACCESSION   DASZMM010000138 DASZMM010000000
VERSION     DASZMM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148009"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VG754_03450"
     CDS             complement(<1..402)
                     /locus_tag="VG754_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWF18292.1"
                     /translation="MPTVTLPKTSKNRLTAKNGNGAAKHDYAVRDLSLAEWGRKTIEV
                     SEHEMPGLMAIRKKYGPLKPLKGVRVTGSLHMTIETAVLIETLATLGASVRWASCNIF
                     STQDHAAAAIAKAGIPVFAHKGESLEEYWDFT"
     gene            complement(426..1604)
                     /gene="metK"
                     /locus_tag="VG754_03455"
     CDS             complement(426..1604)
                     /gene="metK"
                     /locus_tag="VG754_03455"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWF18293.1"
                     /translation="MSKNFIFSSESVGEGHPDKVCDTISDAVLDACLAQDPRSRVACE
                     TYAKCNLVVVGGEITTKAKLDFNAIARAAIREIGYTHDEDVFHADKVLIMNAITSQSP
                     DIAQGVDAKAADGKETAEQGAGDQGLMFGYACNETPELMPAPIMYAHQLGRELTRIRK
                     SGQVHWLRPDAKSQVSVQYENDQPVRITNVVVSTQHAYDVKHKEIKDFIIKEVIKKVL
                     PAHLLDKKTQFLINPTGRFVVGGPQGDTGLTGRKIIVDSYGGMGRHGGGAFSGKDPSK
                     VDRSAAYMGRYVAKNIVAAELATSAEIQFAYAIGYPDPVSVCVNTFGTGIVSDEAIEE
                     AVKEVFSFKPAAIIKQLNLLRPIYSKTTNYGHFGKVDDLNSITWEKTDKAAALKKAVQ
                     "
     gene            1741..3165
                     /locus_tag="VG754_03460"
     CDS             1741..3165
                     /locus_tag="VG754_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18294.1"
                     /translation="MTNPPQISGSPPRPKKNSAWKKWFTRLFILCAIATIAVIVRFSV
                     WRLRLHREINARVASIRAKGLPINWEDLSHWPSDVPDSENAALIYTNAIAHIHFEHLT
                     SKLVNLPMPGEKISENDFSLVKQTVDTNQEGLQLIYSAEKFTKARFPIDYNLGPDAKL
                     PHLSGLRGAARLLAHDAFLKAERKDAEGAAKAAEASLHLSSALENEPLLISQLTSLSI
                     LNESCKSLERVLGKVTFSEPSLLRISTTLSLADATNQILTGLIGDRALNGEYIRLAQD
                     DVRKLVILGNQGADEEDKADVPTRNPGFVWRYLGFFERDRNFYLRAMETNILAVTTPP
                     PASLALTNEVDELSREAQNGLYISSSLFLPSYSRLTGRVATKDATIRTALAAIAIERW
                     RNQHAGVIPDSLDSLVPVYLPSVPVDPYNGEPLKFRKTSKGYVIYSVGPDRQDDGGKP
                     QPLRSPKKQRHPNEHYDITFTVDR"
     gene            3170..4099
                     /locus_tag="VG754_03465"
     CDS             3170..4099
                     /locus_tag="VG754_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWF18295.1"
                     /translation="MSSTLKSLRALSDPTRLRIIALLEREELSVNELQEITRMGQSRI
                     STHLGLLQDAGLLQSRREGKRTFYKLNAEADGTALEFIQLAIRGARELPEYKSDQTNS
                     KRILGRRNEQAQVYFNQVAGRFDRSYGPGRSWQAFGHLLLRILPPVVVADLGSGEGLL
                     SELLARRAKKVIAVDNSEKMVTFGANKAKKNGLKNLEFRLGDLENPPIEPGTVDLVIL
                     SQALHHAVDPAQAIQSAHKILKPGGQLWLLDLMKHKFERAHELYGDRWLGFAESDLHH
                     WLEAAGFKKIEISVVAREEESPHFETILAGGEK"
     gene            4184..>4642
                     /locus_tag="VG754_03470"
     CDS             4184..>4642
                     /locus_tag="VG754_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18296.1"
                     /translation="MKTRIPLGRAFTLIELLVVIAIIAILASLLLPALSSAKEKSHAI
                     ACRSNQRQLAIASYAYSLDFNDWMNPLQDVRTGPDGSQDETTFRVILWDYITHNPSVF
                     DCPSEHVAVYADGISPNDASMGGFSMSSGSDWSSLYGYPSQYEVWNASGIG"
BASE COUNT         1046 a         1195 c         1285 g         1116 t
ORIGIN      
        1 cgtgaagtcc caatattctt cgagcgattc gcccttgtgc gcgaaaacgg gaatgcccgc
       61 tttcgcgatg gccgcggcgg cgtggtcctg cgtcgaaaaa atattgcagc tcgcccaacg
      121 taccgaagcg ccaagtgtcg ccagcgtctc gatgagcacg gcggtctcaa tcgtcatgtg
      181 caacgaaccg gtcacgcgga cgcccttgag cggcttgagc gggccgtatt ttttgcggat
      241 ggccatgagg ccgggcattt cgtgctcgga aacctcgatg gttttccggc cccattcggc
      301 gagcgaaagg tcgcgcacgg cgtaatcgtg tttggcagcg ccgttgccgt tcttggcagt
      361 gagccggttc ttggaagttt ttggcaatgt tacagtgggc atgattttgt tcgttaaatt
      421 gtttattact ggaccgcttt cttgagcgcg gccgctttat cggttttttc ccaggtgatg
      481 ctgttgaggt cgtccacttt tccgaaatga ccataattag tcgtcttgga atagatgggc
      541 cgcagcagat tcagttgctt gatgatggcg gcgggcttga agctgaacac ttccttcacg
      601 gcttcctcaa tcgcttcgtc gctgacgatg ccagtgccaa aagtgttcac gcaaacggaa
      661 accgggtcgg gatagccgat ggcgtaggcg aactggattt ccgcgctcgt cgccaattca
      721 gccgcgacaa tattcttcgc aacgtaccgc cccatgtaag ccgcgctgcg atcgaccttc
      781 gacgggtcct tgccgctgaa cgcgccgccg ccgtgacggc ccatgccgcc gtagctgtcc
      841 acgatgattt tgcggccagt caagccggtg tcaccctgcg gcccgccaac gacaaagcgc
      901 ccggtcgggt taatgagaaa ttgcgttttc ttgtcgagca aatgcgcggg caaaactttc
      961 ttgatgactt ccttgatgat gaaatccttg atctccttgt gcttcacgtc ataggcgtgt
     1021 tgcgttgaaa caacgacgtt ggtgatgcgg acgggttgat cattttcgta ttggacggaa
     1081 acctggctct tcgcgtcggg acgaagccag tggacttggc cggacttgcg gatgcgcgtc
     1141 aattcgcgtc caagctggtg cgcatacatg atgggcgcgg gcatcaattc cggcgtttcg
     1201 ttgcaggcat aaccgaacat caaaccctgg tcgcctgcgc cttgctcggc ggtttccttg
     1261 ccgtcggcgg ccttcgcgtc aacgccctgc gcaatgtccg ggctttgcga agtgatggcg
     1321 ttcatgatga gcaccttgtc cgcgtggaaa acgtcctcat catgagtgta accgatttcg
     1381 cggatcgcgg cgcgggcgat ggcgttgaaa tcgagcttgg ccttcgtcgt gatctcaccg
     1441 ccgacaacca cgaggttgca cttggcgtac gtttcgcaag ccacgcggct gcgcgggtct
     1501 tgcgcgaggc aggcgtccag aacggcatcg gaaatcgtgt cgcacacttt gtccggatgg
     1561 ccctcgccga cggactcgga agagaaaata aaatttttgc tcatttaagt gatgttaacg
     1621 gacggttaaa atgtcatatt gacgtatcag gataagatga tatatccttt tgattgtacg
     1681 tcaactgcta ttttggaaaa tgtggaagta ttattcaaaa ctacggatga ataggaacct
     1741 atgacaaatc cgccgcagat ttcgggttct ccacctcgtc ccaaaaaaaa ttctgcatgg
     1801 aagaagtggt ttactcggtt gttcatctta tgcgcaattg caacaattgc ggtgattgtc
     1861 aggttcagtg tatggcgttt gcgattgcac cgggaaatta atgctcgtgt tgcgtccatt
     1921 cgggccaaag gtttgcccat caattgggag gatttgtcac attggccttc agatgtgccc
     1981 gattctgaaa atgccgcgct catttatacg aatgccatcg cgcatatcca ttttgagcat
     2041 ttaacgagca aattggttaa tctgccgatg cctggcgaaa aaatttccga aaatgatttt
     2101 tcgttggtta aacaaacggt ggatacaaat caggaggggc ttcaacttat atattcggca
     2161 gaaaaattta ccaaggctcg atttcccatc gactataatc tcgggccaga tgcaaaactg
     2221 ccgcatttgt ccgggttgag aggtgcggca cgattgttgg cgcatgatgc ttttttaaag
     2281 gcggaacgga aggatgccga aggtgccgca aaagcggcgg aagcgtccct gcatttgtcc
     2341 agcgcattgg aaaacgaacc actcctgatc tcgcaactta ctagtttgtc cattttgaat
     2401 gaatcatgca aaagtttgga gagagtattg ggcaaagtta ccttttcgga accgtctctc
     2461 ttaaggattt ccacaacact gtccttggcg gatgcgacaa atcagatttt aaccgggctt
     2521 attggagatc gcgccttgaa tggtgaatac atacgcctcg cacaggatga tgtcagaaaa
     2581 ttggtcatct tgggcaacca aggcgctgat gaagaggaca aagccgatgt gcccacgcgc
     2641 aatcctggat ttgtgtggcg ttatttgggc ttctttgaac gcgaccgaaa tttttatttg
     2701 cgcgcgatgg aaacgaatat tttagccgtg accacgccac cgccagcttc acttgctttg
     2761 accaacgaag tggacgagtt gtcacgagaa gcgcagaacg gcctttacat ttcttcctct
     2821 ctgtttctgc cgtcatactc caggctgacg gggcgtgtgg caacaaagga tgcaaccatc
     2881 cgaactgcgc ttgcggctat tgcaatcgaa agatggcgca accagcacgc tggagttatt
     2941 ccagattcgc tggattcact tgtgcccgtt tacctgccgt ctgtgcctgt agatccgtac
     3001 aacggcgaac cgctcaaatt cagaaaaact tccaaaggtt acgtcattta cagtgttgga
     3061 cctgatcgcc aagatgatgg cggcaaaccg caaccgctgc gctcgccgaa aaagcagcgc
     3121 catccaaacg aacattacga cattacattt accgttgacc gataaactta tgtcttcgac
     3181 cctcaaatcc cttcgcgcgc tgtccgaccc gacgcggttg cgcatcatcg cgctgcttga
     3241 acgcgaggag ctttcggtca atgaactgca ggaaatcacg cgcatgggac agtcgcgcat
     3301 ctccacgcat ctcggcctgt tgcaggacgc gggtttgttg caatcgcggc gcgaagggaa
     3361 gcggacgttc tacaaattga acgcggaagc ggatgggacc gcgctcgaat tcatccagct
     3421 tgccattcgc ggcgcgcggg aattgcccga atacaaaagc gatcagacca actccaaacg
     3481 catcctcggg cggcgcaacg aacaggcgca ggtttatttc aaccaggtcg cgggacgttt
     3541 cgaccgcagt tacggacccg gccgttcgtg gcaggcattc gggcatctgc tgttgcggat
     3601 tttgccgccg gtggtggttg ctgatttggg ttccggcgaa ggacttttga gcgaactgct
     3661 cgcgcggcgc gcgaaaaaag tcattgccgt ggacaactcg gaaaaaatgg tcaccttcgg
     3721 cgcgaacaag gcgaagaaaa acggcctcaa gaacctggaa ttccgactgg gcgacttgga
     3781 gaacccgcca attgaaccgg ggacggtgga tttggtcatc ttgagccagg cgctgcatca
     3841 cgcggttgac ccggcgcagg ccattcaatc cgcgcacaag attttgaagc cgggcggaca
     3901 attgtggctg ctcgatttga tgaagcacaa attcgagcgc gcgcatgagc tgtatggcga
     3961 ccgctggctc ggctttgccg agagcgattt gcatcactgg ctcgaagccg ccgggttcaa
     4021 gaagatagag atcagcgtcg tcgcccgcga agaagaatcg ccgcattttg aaaccatcct
     4081 tgctggtgga gagaaataag aatccgagac aaaagtcagg tgccggaatg ttacgttgac
     4141 aatgcgcgcg ggccttctca tattggccgt ggagtgcaat gaaatgaaaa caaggattcc
     4201 acttgggaga gcattcacac tgattgaatt gctggtggtc atcgcaatca ttgcgattct
     4261 cgcctcgctg cttttgcctg ccctgtcgag cgcaaaggaa aaatcacatg ccattgcatg
     4321 ccgctccaat caacgtcaat tggccatcgc ttcatacgct tacagcctgg atttcaacga
     4381 ctggatgaat ccgttgcaag acgtccgcac cggaccggac ggctcgcagg acgaaaccac
     4441 ctttcgcgtc atcttgtggg attatataac gcataaccca agcgttttcg attgtccttc
     4501 cgagcatgtg gccgtttacg cggatggaat ttcccccaat gacgcctcga tgggcggttt
     4561 ttcgatgagt tcggggagtg attggagcag cctttatgga tacccgagtc aatacgaggt
     4621 ttggaatgcc agcggcatcg gc
//
LOCUS       DASZMM010000139        17084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4442450, whole genome shotgun sequence.
ACCESSION   DASZMM010000139 DASZMM010000000
VERSION     DASZMM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 17084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17084
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4442450"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VG754_03475"
     CDS             <1..726
                     /locus_tag="VG754_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18297.1"
                     /translation="GHAEWRLRRDVQEQTCDLRDNALELRGQTGESMADDLVTVFTGR
                     YAALLTRWDGEVSEAFSDKVRVFSRIRRDLLRLQECAREAIRWDREQEKLSMEEKERD
                     KREARQFALAPYRATRRRQHLAKLTGKSGEILADQLDALDYADHPLYPDHFAHSDDKA
                     QKNPDPTPKPKEEKSSLAGKSQATAKPSQPVAAETNQPKTADVESSPVKPISETPPAR
                     LDASFPDPSGPPNPAADGELGRS"
     gene            940..1698
                     /gene="hisA"
                     /locus_tag="VG754_03480"
     CDS             940..1698
                     /gene="hisA"
                     /locus_tag="VG754_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HWF18298.1"
                     /translation="MFRPCIDLHEGKVKQLVGGTLDDAASGLRTNFVSDRSSVYYAEL
                     YKRDGLTGGHVIMLGPGNEEAGRAALKAYPGGLQIGGGINGGNAASYLNDGASHVIVT
                     SWVFQGGNVDWDRLSNLVKTIGKEHLVLDLSCRRRGNEYYVVTDRWQKFTQMTVSKAT
                     LEKLADCCAEFLIHAVDVEGLCRGIDHELVANLSKWSPLPTTYAGGANSLADLEEVTR
                     VGKGKIDLTIGSALDIFGGKGVRYEEAVAFNRKH"
     gene            complement(1909..3834)
                     /locus_tag="VG754_03485"
     CDS             complement(1909..3834)
                     /locus_tag="VG754_03485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HWF18299.1"
                     /translation="MKRPLSSALALALYFLALPLAKSSSILPVSIDRHVAISDAIFQG
                     EVLSLQCWKSEVDGHVYTTAAIRVDQVFKGTLPAVVKLVHFGGTFGNLGEADDFAPVL
                     KVGENRLFFVSRRADGTLYATWGGASALKLPPNGNTPSNPGFIAGQAILARLQKMVSH
                     GVLAGSNVTDQAAASDTTGTSPAPLTYTPSASPMSVATNLLADTNNIAARFVLPDRGE
                     PIPYLIDADYLPTGITLTNAVTAVQTALSAWTAVTSVKYKFMGIQSFGTAAPNILNRD
                     GYLRIQLHDHYNYIGGASGSDVLGKGGHAWDPTVVSGAGWCGGGNVNGSDYYLTVNGF
                     VVLANTNVVMQNLSNFCEVLCHEIGHTLGMAHSSENPSEPNPYLQQAIMYYEIHGNNR
                     GATLGAYDPPVIRQVHPITNTPPYCYNRMMDITTFPSPVTLSNVNVIQLRGYDLQTTN
                     LPFSIANATSNAGSFSFAGSNLTFTPNGYYGASRLDPASGSYYDEVYARCSDGTNASP
                     YIFIRTLSLNPDSYLDGIPDAWRLTYFGSSNPSVGLNHHAINDADGDGYSNLQEYRLG
                     SDPTDKTSNLRVTNFSTTNIQWQAKGYEVYEIYGTTNFSNWVRVMNPVIPTNSFGTAT
                     AFTNGGPKQFFRVEKVQ"
     gene            4100..4348
                     /locus_tag="VG754_03490"
     CDS             4100..4348
                     /locus_tag="VG754_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18300.1"
                     /translation="MARKLYIFKPTGRVKMDGAEPAKWVGGFSTTYAYLGDYQARMLG
                     IDSYFSARRLPAWLHTQPAGAKNAKGASRADRGFRGWG"
     gene            complement(4678..5325)
                     /locus_tag="VG754_03495"
     CDS             complement(4678..5325)
                     /locus_tag="VG754_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18301.1"
                     /translation="MEAAKILIVEDDDLQYEIYEEALSKYKLIRVSNGSQALAVIAKQ
                     TPNVLILDHVLSEGELGLSFLPEFKELLPFVPIIVVSGALEVHQQFRALQGPRRAHYC
                     LPKPVDIHELKKAVATALQECGETEVVRQFEALERSRRVDAHELFSRATDRLSRQNRI
                     REAIGKSTERPNISAFAREFGVARRTIIRDLQELIRRGQLKPEIYPEWENPGEED"
     gene            complement(5338..8532)
                     /locus_tag="VG754_03500"
     CDS             complement(5338..8532)
                     /locus_tag="VG754_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18302.1"
                     /translation="MPTGLHHGHSRLQCDWTCNVPVSSMKFKRWMVSVVGLFLGLTSP
                     CLGQHGSHWRVYRAADGLPETFTASISVGQHGHLWVRHPYVEWVSLLDGYDVKAIPAP
                     ELGTSRIYESAVGQLWTCTIEGLQRYQDTDTGWVHYNLPEVNAFLHTNSPNIYRQTPL
                     YPLRQNHVLLLLPTELLECNVESSEHPQATVMRAANQTGLQKFLGMTPARDGGLWISG
                     AKGLAKIPAPARNLKADTTWEEHIAPVELEMRNFREPVEDEAGGITAVAESLDGNRQV
                     VAHFDGQKWSAQAASEKLLAAWRDLDGTCWASSLPSLLQMRAKEKKFVVDETILARRF
                     WDVVVETNNVFWLATSDGLYRYAPLCWRIPAEFPGQNAFIDAIAEDREERLWVASAAG
                     LNMLHNEHWSSYTFPKDLADSVQEIRVLFPLTNGTIAMEAGNRLLQFNPHNESFHFID
                     APHAGQIKLLGLLKDGALCVQLISTAADGVQSSELQVFDGAGFVAFPYPQPELKQGGE
                     PQMLCVSQNGDLWLSSRKGISRYHEKKWQTFTQSIMQTSGGVTCISEVADGKIWCGIE
                     DRIWEFDGNTWNLIRAGFERVNGITKAADGSVWVASDNGLHRYFHGTWTANGMEEGLP
                     SGAIRSIYQDHRGRYWAATFQGLCEYHPEADPDPPRTHVQNLAERNNTVREGAIVNIS
                     FSAQDKWKYTAEDRLLYSYRLDGQDWSPYQEQRTVSFLDLPAGKHTFSVRSMDRNWNV
                     DPKPARLEFAVALPWFRETRLLLISAAGLGGALFFAAVAFNRHRQLMRSYAEVEAQVA
                     LRTKQLELANHELFHSQKMNALGTLAAGIAHDFNNILSIIKGSAQIIETNLEDQDKIR
                     VRTDRIKTVVEQGAGIVKAMLGFSRTSDHQLTPCDINAVMSDTIKLLGDRFLREIEVQ
                     FTPAPSLPEVPASKDFIQQILLNFIFNAAEAMTEKRRQVILGITQSKQLPATMALKPD
                     AAPSYVMISVKDFGTGISPEIMPRIFEPFFTTKAFSTRRGTGLGLSMVYELTRQMDGG
                     LSVESTLGQGSVFTLILPVRELPMDAPAKSDKVN"
     gene            8799..9683
                     /locus_tag="VG754_03505"
     CDS             8799..9683
                     /locus_tag="VG754_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18303.1"
                     /translation="MKKSSTTWDIRNRTSNGAPCNFHRGFTLIELLVVIAIIATLAGL
                     LLPALHQAKIKAQSAGCLSNLKQVQIAWQIYANDFNDILLPNAPSRTAGVQMATNLPD
                     SMTWCGAAVEGWGALDANTNTAYYLNSILAQFLSQQIRVYKCPGDGLASANGPRLRSY
                     SMNSQMGQYILSQLGPNYAVNFNPGYKLLNTMSDLDCPGPSLAWVFADEHPGSIDDGF
                     LRVNVTSGEWLDVPGSLHGEASIFSFADGHAELHKWLSGIINIPIVQNVSVHNIKGYA
                     NNPDYLWFTQRTACLIGQ"
     gene            complement(9691..11997)
                     /locus_tag="VG754_03510"
     CDS             complement(9691..11997)
                     /locus_tag="VG754_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWF18304.1"
                     /translation="MVHQSAIGPVRRGNLGVCSPGERHGANKSPQITLNWPQDTVLMP
                     YSYTVYRKLLSDTDWGEGTLLDGSVTNFVDTDVADGSAYEYQIVKETSDYTGYGYIYA
                     GMDVPLTEFRGTVLLIVDNTYSAALSGPLAQLQQDLAGDGWTVIRHDVSTNDSVQYVK
                     SLIQTDYNADPADVNTVFLFGHVPVPYSGDIVPDEHVPNHQGAWPADVYYADMTGTWT
                     DYSINDTNADDPRNWNVPGDGKFDQSSPPNPVQLMVGRVDLSNLPGETTWDGPATLPD
                     EQSLLANYLNKDHNYRQKVFYLSRRGIVGNYFGDFGGEAFAASGWRNFAPFFGADNVT
                     DLPNEGTWLPTLSSQAFLWSYGCGAGGYNSIAGLGNDSEYYTAITPDFYNADIQSVFV
                     MLFGSWFGDWDVQDDLMRGVLATPTYGLACMWSGSPHWFCQHMALGQTLGFSTRLTQN
                     NGSNGLYQTEVTTFAGLIHVALMGDPTLRMHVVAPVANLAGDVNGNTVQLSWDSAPDN
                     VVGYNVYRSPGANGPFTRLNDQLVTGNSFTDFNPLTSQVYMVRAVKLETSASGTYYNA
                     GQGIFLPMNVSVGASAVLNVSATTASASRVGLVPGVFTFTRSGDTSEDLTANYSLGGT
                     ANWTVDYKLQPANANSITIPAGESSATLTVVPLPSTNLVGNQTIQLTLLPSLDSEYSM
                     GTQTNALIGLGGNGITGVSLSIFNSTVTLAWPGTDGSAYHVNYENKLDGQWTDFGVDV
                     QGMTGTVFWNDPNTNLARQRFYRVYETQ"
     gene            12884..13327
                     /locus_tag="VG754_03515"
     CDS             12884..13327
                     /locus_tag="VG754_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF18305.1"
                     /translation="MTKSTATLHPYIHFNGRCEEAIKHYQNALGAELVMQMRFNESPE
                     APPPGCLPPGFENKVMHAQMRIGEALLMMTDGPDGQDPTKRDIQGISLSLAVAAEAEA
                     TRYFNGLAKQGKVLMPLAKTFFSPNFGIIADPFGVKWMIYTKPAA"
     gene            complement(13406..14617)
                     /locus_tag="VG754_03520"
     CDS             complement(13406..14617)
                     /locus_tag="VG754_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HWF18306.1"
                     /translation="MNKVKFTWILFLVAFSFAARAGESASFIDAAVIDHSRILKMADA
                     ALTIAPISITKFRAAHSPGSSNDFYSNGDYWWPDPAKPDGLPYIERDGESNPDNFNAH
                     RMAMRDLRDAVASLGAAYRLTGDERYARKAAELLRVFFLDDATRMNPNLSYAQAVPGR
                     TNGRSWGIIDALHLIEIPQAIAAMEKSPAFTPELVAGLKRWFGEMSEWMLTSKNGKQE
                     AAAKNNHAVAFWLQIACYARFTGDEARLAECRRYFKEVFVPEQMATDGSFPLELKRTK
                     PYGYSIFQLDNMAALCQVLSTTNDDLWRFELPDGRGIRKAMAYLYPYLADKSKWPLKP
                     DVQAWEDWPMRQPSLLFAGMAFDESKYLELWKKLPPDSTNAEVRRNVAITQPLLWIQP
                     VGLVEKIKISR"
     gene            15057..>17084
                     /locus_tag="VG754_03525"
     CDS             15057..>17084
                     /locus_tag="VG754_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HWF18307.1"
                     /translation="MKTLFAVLALFCCFAAHAEPEKPLRVFIRAGVKTHGPDQHDYPR
                     FLKEWTPLLNERGAKASGSLQFPTPEQLADADVLVLYCQNAGDITSEQRVSLDAFLKR
                     GGGIVVIHDGVGGHDPQWFKTVVGGAWEYGRSKYFEGDLSFYYLDHSHPITRGVSNFD
                     FDDEMYWDLDLMPEAHVLAGTYQPDDRNKQNGRAMPSIYDIAPQMWTYEKDNYRAFVC
                     IPGHNYKTFNLPHFRAVLLRGIAWAGKRDADLLVGKEDLASVRYPVGGPTAPEEAAKK
                     IFLAPGFNLRLVASEPLIEKPISMDWDAKGRLWIAETPEYPFRKDRSREPYDRISILE
                     STRNDGRMDKKTVFYDKLDLVTSLVFYKDGVIVSQAPEILWLRDTKHKDKADERIVLY
                     KGFGTFDTHAVLSNLRWGMDGWIYGTVGYTRGDIYSGDGKTHFGKISDGVFRFKPDGS
                     AIEQVSSKGGNTWGVDIAPDGEIFYSQANGNHVDHLVMPESALARGRVGNATSYLNIE
                     DHNRSFPMMSWTKQAYQQIDWVGNFTAASGACIYDGGAWPQKYDYTYYVSEPTINIVH
                     QDFLRPKGVSYVASKDPQRLEQEFIASTDLWFRPIHQRVGPDGALYILDFYNQAVVHN
                     DTRGPRHDPQGNAAIRPDRDHYFGRIWRVQHDDAKKLQIPKLDPPKTSELVD"
BASE COUNT         3950 a         4640 c         4697 g         3797 t
ORIGIN      
        1 ggtcatgccg aatggcgctt gcgccgggat gtccaggaac agacctgcga ccttcgcgac
       61 aatgccctcg aactccgcgg ccaaaccggc gagtcgatgg ccgatgacct ggtcaccgtc
      121 ttcaccggcc gttacgccgc cctgctcacc cgttgggacg gcgaagtctc cgaagccttc
      181 tccgacaagg tgcgcgtgtt cagccgcatc cggcgagatt tgttgcgctt gcaggaatgc
      241 gcgcgagagg ccatccgctg ggaccgcgag caggagaaat tgagcatgga agaaaaggag
      301 cgcgacaaac gcgaagcgag gcagtttgcg ctggcgccct accgcgccac ccggcgacgc
      361 caacacttgg cgaaactcac gggcaagtcg ggcgaaatat tggccgacca gctcgacgcc
      421 ctggattacg ccgaccatcc cctttatccc gatcacttcg cccactccga tgacaaggcg
      481 cagaaaaatc ctgacccaac acccaaaccc aaggaagaga agtcaagcct tgctgggaaa
      541 agtcaagcca cggcaaaacc gtcccaaccc gttgctgctg aaaccaatca acccaaaacc
      601 gccgatgtcg agtcaagccc ggtcaagcca atttctgaaa cgccgccagc acgacttgac
      661 gcttcgtttc cagacccttc cggtccgcca aatccggcag cggatggcga gcttgggcga
      721 tcttgaagga tgaaatggtg ggacgagcgt accgcgagcc tcaaaagctg acttgatcaa
      781 ggcgcaaaca ggacgccaat ggatgagaac tgccataaga cgtacaagcc ccggcttgcg
      841 attacgctcg cctacccgcc gcaaaatata cctgcgctac gggaggacaa ggcttttcaa
      901 ctttaaacct taaactttaa accttaaact catattgaaa tgtttcgtcc ctgtattgac
      961 ttgcacgaag gcaaggtgaa gcaactcgtc ggcggcacgc tggatgatgc ggcgtcgggc
     1021 ttgcgcacca attttgtctc cgaccgttcc agcgtctatt acgccgagct ttacaaacgc
     1081 gatggattga ccggcggcca tgtcatcatg ctcggcccgg gaaacgagga agcgggtcgc
     1141 gcggcgctca aggcgtatcc gggcggattg cagatcggcg gcggcatcaa cgggggcaac
     1201 gccgcgagtt atctgaatga cggcgcgtcg catgtcatcg tgacgtcgtg ggtgttccaa
     1261 ggcggaaacg tggattggga ccggttgtcg aacctagtga aaacaatcgg caaggagcat
     1321 ctcgtcttgg acttgagctg ccggcgtcgc gggaatgagt attacgtggt gaccgatcgc
     1381 tggcaaaagt tcacacagat gaccgtgagc aaggcgacgc tggaaaagct ggcggattgt
     1441 tgcgcggaat ttttgatcca tgcggtcgat gtggaaggct tgtgccgcgg cattgaccac
     1501 gagttggtgg cgaacctcag caaatggtcg ccgttgccga cgacttatgc gggcggagca
     1561 aattcgctcg ccgaccttga ggaagtgacg cgcgtcggca aagggaagat tgacctgacc
     1621 atcggctcgg cgctggatat ttttggcggc aaaggagtgc gttacgagga ggcggtggcg
     1681 tttaatagga aacattgaac gtcgaatgtt gaacatcgga cgcttagatg aaaatgagaa
     1741 taatagtgat tacgctcgcc ctcacccctg ggagagggag aaccatttgc cgccgctcga
     1801 aataaaaccg ctccccggtt ttcaatcttt tcaaatgaca acctgaacat cgaacgctga
     1861 aagtcaaatg tttacttcat cgaagtaacc aagcaggtct cctttatgtc actgcacctt
     1921 ttcaacgcgg aagaattgtt tcggcccgcc attggtgaaa gcggtggctg tgccgaagga
     1981 gttggtcggg atcaccgggt tcatgacacg gacccagttg gaaaagttgg tggtgccata
     2041 aatttcatag acctcatagc ccttcgcctg ccattggatg tttgtcgtgg agaaattcgt
     2101 gacgcgcagg ttggaagttt tgtccgttgg gtccgagccg agccgatatt cctggagatt
     2161 actgtaacca tccccatcgg cgtcgttgat ggcatggtga ttcaagccaa cggatggatt
     2221 ggaactgcca aaataggtca accgccacgc gtcgggaatg ccgtccaaat aagaatccgg
     2281 attcagggac aatgtccgga tgaagatgta tggggaagca ttggtgccat ccgaacagcg
     2341 ggcgtaaacc tcgtcgtaat aactgccgga ggcgggatca agtctcgacg cgccgtaata
     2401 accgttcggc gtgaaggtca ggttgcttcc tgcaaacgaa aaagaaccgg cgttggatgt
     2461 ggcgttcgcg atggagaacg gaagattggt tgtttgcaga tcataaccgc gcagttggat
     2521 gacgttcacg ttggaaagag tcaccggact tgggaatgtg gtgatgtcca tcatccgatt
     2581 gtaacaatag ggcggagtgt tggtgatcgg atgcacctgc cggatgaccg gcgggtcata
     2641 cgcgccaagg gtcgcgcccc ggttattgcc atggatctca taatacatga tggcctgctg
     2701 aaggtaaggg ttgggctcac tgggattttc gctcgaatgc gccataccaa gcgtgtggcc
     2761 gatttcgtgg cagagaactt cgcagaaatt cgagaggttt tgcatgacta cattggtgtt
     2821 cgccaaaacg acaaagccat tgacggtcag gtaataatcg ctgccgttca cgttgccgcc
     2881 gccgcaccaa cccgcgccgg aaacgacagt cgggtcccac gcgtgaccac ctttgccaag
     2941 gacatcggaa ccgctcgcgc cgccgatgta attgtaatga tcgtggagct gaatgcggag
     3001 gtaaccgtcg cgattcaaga tattcggagc ggccgtgcca aaactttgga tgcccatgaa
     3061 tttgtatttg acgctcgtta cggccgtcca cgcggaaagg gcggtttgca ccgcagtcac
     3121 cgcattggtc agtgtgatgc ccgttggcag gtaatcggca tcgatgagat aaggaatcgg
     3181 ttcaccgcgg tcgggaagga caaatcgggc cgcgatgttg ttggtgtccg ccagcaaatt
     3241 cgtcgcaacg gacatcggcg aagccgacgg agtatatgtc aagggcgccg gactggttcc
     3301 ggtggtatcc gaagcggcag cttgatcagt cacgttgctg ccggcaagga ctccgtggga
     3361 aaccattttt tgaagccgcg ccagaatggc ttggccagca atgaacccag gattggaggg
     3421 agtattaccg ttcggcggca atttgagagc gcttgctccg ccccaggttg cgtacagcgt
     3481 gccgtccgcc cggcgagaaa caaagaaaag acggttctcg cccaccttga gcacaggcgc
     3541 gaaatcgtcc gcttcgccca agttgccgaa tgttccgccg aaatgaacga gtttcacgac
     3601 cgcaggcaag gtgcctttga agacctgatc cacccgaatg gctgccgtgg tatagacatg
     3661 gccgtcaact tcgcttttcc aacattgcaa actcaacact tcaccttgga aaatggcatc
     3721 ggaaatcgcc acgtgccgat caatgctgac tggcaggatg gaggaggatt ttgccaatgg
     3781 caaagccaag aaatacaaag cgagcgcgag agccgaagac aatggtcgtt tcataggtgg
     3841 atgaaatggc ttcaattcgt tgaaaaacga aggcgattta aaacagcttc agcgaacatt
     3901 gcaatgaccg agttccgtcc agaaaaagta cgcggtgtca tcggttttgt acgaggggtg
     3961 cgtgaaaatc acgcgcgttt gcgtggggca gaaacagttt gttatctcct gcaagtcaat
     4021 aacctgcgaa ttattggggt ttttgagttg gcatcgtgcc tgcttaaaaa caggcaacgc
     4081 agcaaagatt catcagctta tggcgcgcaa actatacata ttcaaaccga ccggaagggt
     4141 gaaaatggat ggagcggagc cggcgaaatg ggtgggtgga ttttccacga cctacgctta
     4201 tttgggcgat taccaggcga ggatgcttgg gatagacagt tacttttcag ccagacgatt
     4261 gcccgcatgg ttgcacactc aaccggcggg tgccaagaac gccaaaggcg cgagccgggc
     4321 agatcgtggg tttcgggggt ggggctgagc catctgggcg cgaatctgtg ggggtgcgcc
     4381 cagatggtct cgatttcccg cggcagtgag cgggggaaca ggttggtcgc aagtcagttc
     4441 actgcatcgt tgttagtccg cagtattagt agcagtagtc agtggtttga gttgataggt
     4501 ggagtgggac ggaagggtgc ttcggtgttc aacgaagcgg atctggtgcg tgccagacgc
     4561 ttcggggctc accgaagccc tttctctttt tggaggaaca tcgaacctcc aacgctgaac
     4621 gctgaacatc gaaagctcaa tcgtcccaaa gcattcggtt ggaaacagga ctggcgctta
     4681 atcctcttcg ccgggatttt cccactcagg atatatctcc ggtttcaatt gaccgcgccg
     4741 gatgagttcc tgcaaatcgc ggatgatggt gcggcgggcg acgccgaatt cgcgggcaaa
     4801 agcggaaatg ttcggccgct cggtggactt gccaatggct tcgcggatgc ggttttgccg
     4861 gctcagacga tcggtggcgc ggctgaagag ttcgtgggcg tcaacgcgac gcgagcgttc
     4921 gagcgcttcg aattggcgca caacttcagt ctcgccgcat tcctgcaagg ccgtggcgac
     4981 agcttttttg agttcgtgga tgtccaccgg tttgggaagg cagtagtgag cgcgacgcgg
     5041 gccttgcaat gcgcggaatt gttgatgcac ttcaagcgcg ccggaaacca cgatgatggg
     5101 aacgaacggc agcaattcct tgaactccgg caggaagctc agtccgagct cgccttcgga
     5161 caaaacgtga tcgagaatca ggacgttggg agtctgtttc gcgatcacgg ccagcgcctg
     5221 gctgccgttg gacacgcgga tcaacttgta tttggacagc gcttcttcgt aaatctcgta
     5281 ttgaaggtcg tcgtcctcaa cgatcaggat tttagccgct tccataggtt tttggattca
     5341 gttcacttta tcgctttttg ccggggcgtc catcggcaat tcccgcaccg ggaggatgag
     5401 agtgaaaacg ctgccttggc cgagtgtgga ttccacgctc aagccgccgt ccatctggcg
     5461 cgtcaattca taaaccatgg acagaccgag tccagtgccg cggcgggtgg agaatgcctt
     5521 cgtggtgaaa aaaggttcga aaattcgcgg catgatttcc ggcgaaatgc cagtgccaaa
     5581 atctttcacc gaaatcatga catacgaggg cgcggcatct ggtttgagcg ccatggttgc
     5641 cggcaattgt ttggattgcg tgatgcccaa aatgacctga cggcgttttt ctgtcatcgc
     5701 ttcggcggcg ttgaagataa agttgagcaa tatctgttgg atgaaatcct tggacgcggg
     5761 gacttccggc aacgacggcg caggagtgaa ctgcacttca atttcacgca aaaaacggtc
     5821 gccgagcaat ttgatggtgt cggacatcac tgcgttgatg tcacaaggtg tgagttgatg
     5881 atcagacgta cggctgaaac cgagcatggc tttgacgatg cccgcgcctt gttcgacaac
     5941 agttttgatg cggtccgtgc ggacgcggat tttgtcctgg tcttcaaggt tcgtctcgat
     6001 gatttgcgcc gaacctttga tgatggaaag gatgttgttg aaatcatgcg caatcccggc
     6061 ggcgagcgtg ccgagcgcgt tcattttctg gctgtggaac aattcatggt tggccagttc
     6121 cagttgtttc gtgcgcaacg cgacttgggc ttcgacttca gcgtagcttc gcatcagttg
     6181 acgatgtcgg ttgaacgcca ctgctgcaaa gaaaagcgcg ccgcccaatc ccgccgcaga
     6241 aatcagcagc agccttgttt ctctgaacca cggcagggca acggcaaact ccagtctcgc
     6301 aggtttggga tccacgttcc agttgcggtc catgctgcgc acagaaaatg tgtgcttgcc
     6361 tgccggcaaa tcgaggaacg aaactgttcg ttgctcctga tacggagacc aatcctgtcc
     6421 atccaatcga taggagtaca acaggcgatc ttccgccgtg tatttccatt tatcttgcgc
     6481 gctgaacgaa atgttcacga tggcgccttc acggacagtg ttgtttctct cagccaggtt
     6541 ttgaacgtgg gtgcggggtg gatcgggatc cgcttcggga tgatactcgc aaaggccttg
     6601 aaaagttgcg gcccaatagc gtccacgatg gtcctggtaa atcgaacgaa tcgcgccgct
     6661 gggcagtcct tcctccatcc cgttggccgt ccaagtgccg tgaaaatacc tgtgcaagcc
     6721 attgtcggaa gccacccaaa cgcttccgtc agcagccttg gttatcccat tcacccgctc
     6781 gaatccggcg cggatgaggt tccatgtgtt cccgtcaaac tcccagattc tgtcttcgat
     6841 tccgcaccaa atctttccat cggccacttc cgaaatacac gtcaccccgc cggatgtttg
     6901 cattatggac tgcgtgaatg tctgccattt tttttcatga tatctggaaa tccctttgcg
     6961 gctgctgagc caaaggtcgc cgttttgcga aacacagagc atttgaggct cgcctccctg
     7021 cttcaactcc ggttgcggat acggaaacgc gacgaaacca gcgccatcga atacctgcaa
     7081 ttcagaagat tgaacgccat cagcggcggt gctgatcaat tggacgcaca acgcgccgtc
     7141 cttgagcagg ccgagcaatt tgatctgccc tgcgtgaggt gcatcgataa aatgaaaact
     7201 ttcgttgtgc ggattgaact ggagcaggcg atttcctgct tccatggcga tggttccgtt
     7261 cgtcagcggg aaaagaacac ggatttcctg aacactgtcc gcgaggtcct ttggaaaagt
     7321 gtaactactc caatgctcgt tgtgcaacat gttcaatccg gctgcgcttg cgacccagag
     7381 tcgctcttcg cgatcttccg cgatggcatc aataaaggca ttctgaccag ggaattccgc
     7441 gggaatgcgc caacacaatg gcgcgtaacg atacaatcca tcggaagtcg ccagccaaaa
     7501 cacgttgttt gtctcgacaa caacatccca aaaccgcctc gcaagaatcg tctcatccac
     7561 cacgaacttt ttctcttttg cccgcatttg cagcagggaa ggcagactgc tggcccagca
     7621 ggtcccatcc aaatctcgcc aagctgcaag cagcttttcg ctggccgcct gtgccgacca
     7681 tttttgcccg tcgaaatgcg ccaccacttg ccggttgcca tccaaagatt ccgcaaccgc
     7741 agtgatgcct ccagcttcgt cttcaaccgg ctcacgaaaa ttccgcatct ccagttccac
     7801 cggtgcaatg tgctcctccc acgtcgtgtc cgctttcaaa ttgcgcgccg gagcggggat
     7861 tttggcgagg ccctttgcac cggaaatcca aagccctccg tcgcgcgccg gagtcatgcc
     7921 gagaaacttt tgcaggccag tttgattggc agcgcgcatg actgtggctt ggggatgttc
     7981 cgatgattcg acattgcatt ccaatagttc ggtgggtagc agcaaaagca catggttctg
     8041 gcgcagggga taaagcggcg tctgcctgta tatgttaggg ctgttcgtgt gcaagaatgc
     8101 gttaacttcc ggcaggttat agtgaaccca cccggtgtcc gtatcttgat agcgttgcaa
     8161 tccttcgatt gtgcaggtcc ataactggcc cacagcgctc tcgtaaatcc ggcttgtccc
     8221 caattccgga gcaggaatgg ctttgacatc gtaaccatca agcaagctga cccattcgac
     8281 ataaggatgt ctcacccaca aatgcccgtg ctggccaacg ctgatggaag ctgtgaatgt
     8341 ctccggcaat ccatccgcag cccgataaac gcgccaatgc gagccatgct gccccaagca
     8401 gggcgaagtc agcccgagga agagtcccac aactgacacc atccagcgtt tgaacttcat
     8461 gctcgatact ggaacattgc acgtccagtc acattgcaac cggctgtgtc cgtgatggag
     8521 tccagtcggc atgattattc aacgattggc gcgagtgtgc aatgaaaagc aggggtgtgc
     8581 tgccagacgc acagttccca agctgcgcgg cctgtgcgaa tcattggcat gatttgccgg
     8641 gcacggtcgt ctccacgcgc agcaaagcca gggggcggaa cggcggactg gttcaaaagc
     8701 cggaccgaaa tcagaatgcc gcgcttgcgg gcggccgcag tccggcaagt cagcgcaacg
     8761 ctaaccctca ctccaccaat tcgctgtcca gtcgaagcgt gaaaaaaagc tccacaacgt
     8821 gggacatacg aaatcgaacg tcgaatggcg cgccgtgcaa ttttcatcgc ggcttcacct
     8881 tgattgaact gctcgtggtt atcgctataa ttgcgacttt ggccggattg cttctccctg
     8941 cattgcatca ggcaaaaatc aaagcccaaa gcgccggttg tctcagcaac ctcaaacaag
     9001 ttcaaatcgc ctggcagatt tacgccaacg atttcaatga catcctgctt cccaatgcgc
     9061 catcccgaac ggccggcgtt cagatggcca caaaccttcc cgattccatg acttggtgcg
     9121 gtgccgctgt ggaaggctgg ggcgctctcg acgccaatac gaataccgcc tactatctca
     9181 actccatcct cgctcaattc ctttcccaac aaattcgcgt ttacaaatgc ccgggtgatg
     9241 gccttgcttc cgccaatgga ccccgtcttc gcagttattc catgaacagc cagatgggtc
     9301 agtacatcct ctcgcaattg ggccccaact atgccgtcaa tttcaatccc ggctataaac
     9361 tcctcaacac catgagcgat ctcgactgtc cgggaccgtc tttggcgtgg gttttcgcgg
     9421 acgaacatcc cggcagcatt gatgacggtt tcctgcgcgt gaacgtaacc agcggagaat
     9481 ggctggatgt tcccggctct ttgcacggtg aggcctcgat tttttcgttc gctgacggtc
     9541 atgccgagct tcacaaatgg ctcagtggaa tcatcaatat tcccattgtc cagaacgtgt
     9601 cggtgcacaa catcaaaggg tatgcgaaca atcccgacta cctctggttc acccagcgga
     9661 cggcctgcct cataggccag tgacgattgc ctattgagtt tcataaacgc gatagaaacg
     9721 ttgccgcgcc agatttgtat tcggatcgtt ccagaaaact gttcctgtca tgccttgcac
     9781 atccacacca aaatccgtcc attgaccatc aagcttgttt tcataattca catgatatgc
     9841 gctgccatca gtgccgggcc aggcaagtgt caccgttgaa ttgaaaatcg aaagcgaaac
     9901 gcccgtgatt ccatttccgc cgaggccaat gagagcatta gtttgcgttc ccatcgaata
     9961 ttcggaatcc agggatggaa gcaaggtgag ttgaatagtt tgatttccaa ccaaattcgt
    10021 gctgggcagg ggaaccacag tgagcgtcgc tgaactttct cctgcgggta tcgtgatcga
    10081 gttcgcattg gccggttgca gtttataatc caccgtccag ttggctgtcc cgccaagcga
    10141 ataattcgcg gtcaaatctt cgctggtgtc gcccgaacgc gtgaaggtga acacgcccgg
    10201 cacaagacca acgcgcgaag ctgatgccgt cgttgccgaa acattgagca ctgcggatgc
    10261 gccgacgctc acattcatcg gcagaaaaat tccctggccc gcgttataat aagtgccgct
    10321 ggcgctcgtt tcaagtttaa cggcgcgcac catataaact tgtgacgtca aaggattgaa
    10381 atctgtgaaa ctattgcccg tcaccaattg atcattcagc cgggtgaaag gcccgttggc
    10441 accgggcgaa cgataaacat tataaccaac cacattgtcg ggcgccgaat cccaactcaa
    10501 ttggactgtg tttccattca cgtcgcccgc gagatttgca accggggcaa cgacatgcat
    10561 ccgcaaagtc ggatctccca tgagcgccac gtgaatcaaa ccggcaaaag tcgtgacctc
    10621 ggtttgatag aggccgttcg agccattgtt ttgggtcagc ctggtgctga agccaagcgt
    10681 ttgtcccaac gccatgtgct ggcaaaacca atggggactt ccgctccaca tgcacgccag
    10741 cccgtaagtt ggcgtggcaa gcactccgcg catcaagtcg tcctgcacgt cccaatcacc
    10801 gaaccagctc ccgaacaaca tgacaaacac cgattgaatg tccgcgttgt aaaagtccgg
    10861 ggtgatggcg gtgtaatatt cactgtcatt gcccaagccg gcgatggaat tgtatccgcc
    10921 cgctccgcag ccgtaagacc agagaaacgc ctgcgaactg agcgttggaa gccatgtgcc
    10981 ttcgtttggc aaatccgtga cgttgtcggc tccgaagaac ggagcgaagt tccgccagcc
    11041 gctggcagca aaagcctcgc cgccaaaatc gccaaaataa tttcccacga tcccccggcg
    11101 cgacaggtaa aaaactttct gacgatagtt gtggtctttg ttcaaataat tggcgagcag
    11161 gctttgttca tcgggcaatg tcgccggacc gtcccaagtt gtttcgccgg gaagattgga
    11221 aagatcgacg cgaccaacca tgagctgaac tgggttgggc ggagaggatt gatcaaattt
    11281 cccgtctccc gggacattcc aattgcgtgg atcatcggca ttggtgtcat tgatggagta
    11341 atccgtccaa gtgccggtca tgtcggcgta atagacgtca gccggccagg cgccttgatg
    11401 attcggcaca tgttcatccg gcacgatatc gccggaataa gggaccggaa cgtggccgaa
    11461 gagaaacacc gtgttcacat cggcggggtc tgcgttgtaa tcggtttgga tgagcgattt
    11521 cacatattga acgctgtcat ttgtcgagac atcatgccgg atcaccgtcc aaccatcgcc
    11581 ggcaaggtcc tgctgcaatt gcgcaagcgg cccgctcaat gccgctgaat acgtgttatc
    11641 cacgatgagc agcaccgtcc cgcgaaactc tgtcaacggc acatccatgc cggcatagat
    11701 gtaaccgtaa cctgtgtaat ctgaggtctc tttcacgatt tgatattcgt atgccgaacc
    11761 gtctgcgaca tctgtgtcca caaagttggt gacggaacca tccagaagcg tgccttcgcc
    11821 ccaatcagtg tcggaaagca actttcgata aacggtatag ctgtaaggca tgagaaccgt
    11881 gtcctgcggc cagttcaatg tgatctgtgg agacttgttt gcaccgtggc gctcacccgg
    11941 actgcatact cccaagttgc ctcttctgac cgggccgatg gcggactgat gaaccacaaa
    12001 aatggcaaaa tcaaaccggc ccaaacccac ctcgcgcgca cgtctgtttt caaattgacc
    12061 ttcctgtatt gggcgtcaat ttccgcgacc aagccgggtt ttcaaatggg gtgataaacc
    12121 tgccataaat ggccaagcga cactaagcga aacgctgcgg aacgcgggtt ttcaagtact
    12181 tacaacttgg acgcttgatg ctcaatgccg aattctcgag gcttgaacgc tttcccggca
    12241 ccactccgca atcggcaggg gaggggtccc ctttttatgc gaaccgatgc gggtctaagc
    12301 ggttttaagc gggtttacaa attcttacaa cttctcgctc gatgctggac gcttgatgtc
    12361 caatcaccgc aactcggaac cgattacatc aactatcaac cacccacaat caacgatttt
    12421 ttaggatgtc aaagaacttg tccgcctcct tccaacgccg ccactttagc acgcctgtca
    12481 agcggctttt tcttgagttc ctaaatcggt tctttttatt acaacacgcc tcataaataa
    12541 agaacggagc gcgactgtgt caccaggacc agtcgcagga gcttgggacc attcaatgtt
    12601 ttggcaggtt gctgcggctg gcgcttcgcg cacagccgcg ctccacagat ttacgagatg
    12661 tgtgttctag gaacacagga actcaggaaa aaatggaagg gcaaaagcat cctgttcctg
    12721 agttcctgat ttcctaatct tttcggccca taaagaattc aaattcgttg tcctcaatgc
    12781 ccttgctcat tcgtcatatc attgaaagca cggttgattt tgtgaccgat gcgaaaaaat
    12841 gaagtcatta ttaatagcaa acaacgaaga aagaccacac gttatgacca aatcaactgc
    12901 aacactccac ccttacattc atttcaacgg acgctgcgag gaagccatca agcattatca
    12961 aaacgccctc ggggccgaac tcgtgatgca aatgcgcttc aatgagagtc ctgaagcgcc
    13021 gccgccgggc tgtttgccgc ccgggtttga gaacaaagtc atgcacgcgc agatgcgcat
    13081 cggcgaagcc ctcctgatga tgaccgatgg ccccgacgga caagacccaa ccaaaaggga
    13141 catccagggc atctcgttga gcctggcggt tgccgccgaa gccgaggcaa cccgctattt
    13201 caacgggctg gcaaaacagg gaaaagtcct gatgccgctg gcaaaaacct tcttttcccc
    13261 gaatttcggc atcatagccg accccttcgg cgtcaaatgg atgatttaca ccaaaccggc
    13321 agcgtaaaac gcgctcccaa tccaacggga ttgcatcaat cagcccaggg ttgccgtgca
    13381 gcggcgacct tgggccagcc gtttttcatc gtgaaatttt aattttctcc acaagcccga
    13441 ccggttgaat ccacagcaac ggttgcgtga tggcgacgtt gcggcggact tcggcattgg
    13501 tcgaatcagg cggaagtttt ttccacagct ccaaatattt cgattcgtcg aacgccatgc
    13561 ccgcgaacag caggctcggt tgccgcatgg gccagtcttc ccacgcctgc acgtcgggct
    13621 tcaacggcca tttcgatttg tccgcgagat atggatacag ataggccatc gccttgcgga
    13681 tgccgcgtcc atccggcaat tcgaaacgcc atagatcatc attggtcgtc gaaagcacct
    13741 ggcaaagcgc cgccatgttg tccaattgga aaatggaata gccgtagggt ttggtgcgct
    13801 tgagttccag cggaaaactg ccgtccgtgg ccatctgctc cggcacgaac acctccttga
    13861 aataccggcg gcattcggca agccgcgctt cgtcgccggt gaaccgcgcg taacatgcaa
    13921 tttgcagcca gaatgccacg gcatgattgt tcttcgccgc ggcttcctgt tttccgttct
    13981 tgctggtcag catccattcg ctcatttcgc cgaaccatcg tttcaagccc gcgacgagtt
    14041 cgggcgtgaa tgcaggcgat ttttccatcg cggcgattgc ctgcgggatt tcaatcaaat
    14101 gcagcgcgtc aatgatgccc caggatcgtc cgttggtgcg gcccggaacg gcttgcgcgt
    14161 acgacaggtt cggattcatt cgcgtcgcgt cgtcgagaaa gaaaacgcgg agcaattccg
    14221 ccgccttgcg cgcgtaacgt tcatcgccgg tgagccggta agccgcgccg agcgatgcca
    14281 ccgcgtcccg caaatcacgc atcgccatcc ggtgcgcgtt gaaattgtcc ggatttgatt
    14341 cgccgtcgcg ctcgatgtaa ggcagaccgt ccggctttgc cgggtccggc caccaataat
    14401 cgccgttgga ataaaaatcg ttcgagcttc ccggactgtg cgccgcgcga aatttcgtga
    14461 tgctgatggg cgcgatggtc aacgccgcgt ccgccatttt caatatgcga gagtggtcaa
    14521 tcacagccgc gtcaatgaaa ctggcggatt ctccggcacg agcggcgaag gagaacgcaa
    14581 ccaggaataa aatccaggtg aatttcactt tgttcatgag catcatggaa acgtgaaacc
    14641 ggcgcttttc atttggctgt taagaggttc tttcgccgtt tttaggatga tagtcaagtg
    14701 agtgtgaggt gaggaatgca tctctgtact gaaactgcag gagggcgcat cttgatgctg
    14761 ccgccgaagt ttattgcttc gcggccaaag agtagcgcag atttttaatc tgctgtatcg
    14821 ccgacttgca gtcggcggca cggtcaaaaa cacggatagc tttggaattt ctcaaatcgt
    14881 gattccatcg cgcgggcttg cggagtaaaa ctccgcgata cagcagatta aaaatctgcg
    14941 ctacgggact ggcgtcagcc tgcgatgagt ttctgatata gcggggctga gaccagccaa
    15001 ataattcttc cgggaagacg ttgtttggct taacctcagt tcacagcttt caaacgatga
    15061 aaactttgtt tgccgtcctc gcgctttttt gctgttttgc tgcccacgca gaaccggaaa
    15121 aaccgttgcg cgtgttcatc cgcgccgggg tgaagacgca cggcccggat caacatgatt
    15181 atccgcgttt tctcaaggaa tggacgccgt tgctcaatga acgcggggca aaggcttccg
    15241 gttccctgca atttcccacg ccggaacaat tggccgatgc ggacgtgctc gttctctatt
    15301 gccaaaacgc gggcgacatc acatcggagc agcgcgtttc gctcgacgca ttccttaaac
    15361 gcggcggcgg cattgtggtc atccatgacg gcgttggcgg gcatgatccg cagtggttca
    15421 agaccgtcgt cggcggcgca tgggaatatg gccgctccaa atatttcgag ggcgaccttt
    15481 cgttttatta cctggaccat tcgcatccca tcacgcgcgg cgtttccaat ttcgatttcg
    15541 acgatgaaat gtattgggac ctggacttga tgccggaagc gcatgtgctc gcgggcactt
    15601 accaacccga cgaccgcaac aaacaaaacg gcagggcgat gccgtccatc tacgacatcg
    15661 cgccgcaaat gtggacttac gagaaggaca attaccgagc gtttgtctgc attcccggcc
    15721 ataattacaa gacgttcaac cttccgcatt ttcgcgcggt gctgctgcgc ggcatcgcct
    15781 gggcgggcaa acgtgacgcg gatttgctgg tgggcaagga agatttggcg tcggtgcgtt
    15841 atcccgtcgg cggcccgact gcgccggaag aagcagcaaa aaaaatcttt ctcgcgcccg
    15901 gattcaattt gcgactggtc gcgtcagagc cgttgattga aaaaccgatc tcgatggatt
    15961 gggacgcgaa agggcggctt tggatcgcgg aaacgccgga gtatccgttc cgcaaagacc
    16021 gttcgcggga gccgtatgat cgcatttcca tccttgaatc cacgcgcaac gacgggcgca
    16081 tggataagaa aacggttttc tacgacaagc ttgatctggt cacgagcctg gttttttaca
    16141 aggacggcgt catcgtctcg caagcgccgg aaatcctttg gttgcgcgac acgaaacaca
    16201 aagacaaggc ggatgaacgc atcgttttgt acaagggttt cggcacgttc gacacgcacg
    16261 cggtcttgag caatttgcgc tggggaatgg atggttggat ttacggaacg gtcggttaca
    16321 ctcgcggcga tatttattcc ggcgacggca aaacgcattt tggaaaaatc agcgacggcg
    16381 tctttcgttt caagccggac ggcagcgcca tcgagcaggt cagttccaag ggcggcaaca
    16441 cctggggcgt ggacatcgcg ccggacgggg aaatatttta ttcgcaggca aacggcaatc
    16501 acgttgacca tcttgtgatg ccggaatcgg cgctcgcgcg cggccgcgtc ggaaatgcca
    16561 ccagttacct gaacatcgag gaccacaatc gcagtttccc gatgatgtcg tggacgaaac
    16621 aggcgtatca gcagattgat tgggtcggga atttcaccgc agcgtcgggc gcgtgcattt
    16681 acgacggcgg cgcatggccg caaaaatatg attacactta ttacgtttcc gaaccaacca
    16741 tcaacatcgt ccatcaagat tttttgcggc cgaaaggcgt gagttacgtc gccagcaagg
    16801 acccgcaacg gcttgaacag gaattcattg cgagcaccga tctttggttt cggcccattc
    16861 accagcgcgt ggggcctgac ggcgcgcttt acattttgga tttttacaat caggcagttg
    16921 tccacaacga cacgcgcggc ccacgtcacg atccgcaagg caacgcggca atccgccccg
    16981 accgcgacca ttatttcggc cgcatctggc gcgtgcaaca tgatgatgcg aaaaaactac
    17041 aaattccgaa acttgatcct cccaaaacgt ccgaactggt ggat
//
LOCUS       DASZMM010000140         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1148452, whole genome shotgun sequence.
ACCESSION   DASZMM010000140 DASZMM010000000
VERSION     DASZMM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148452"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..608
                     /locus_tag="VG754_03530"
     CDS             57..608
                     /locus_tag="VG754_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18308.1"
                     /translation="MLALLLAGLSWPAFAREMTAYQLVKEADRYVGEDVKDQVVELRS
                     EKSIGGLVPNIWFVTFYDPDATFKATEIKFGAGKKLEVKRPARVLEWNTKDSQKLDPK
                     KLNVDSDKAISIATKEPVLKGLNLKATQLWLEHSSDGPVWKVRIWAAKLRNPNDDANL
                     GEVYVKADDGTVTKDDLHIDRVD"
     gene            702..2273
                     /locus_tag="VG754_03535"
     CDS             702..2273
                     /locus_tag="VG754_03535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF18309.1"
                     /translation="MNKDNPAETTFDVVIVGGALSGAATAVLLLKEKPDLRVLIVEKS
                     TAFTRRVGEATIEVSTYFLTHSLGLTGHLTESHLSKQGLRFWFANEKTQTLGDCSEIG
                     GRYLARVPAFLVDRAVMDQEVLRRACAMGAQLWRPATVQKIELVLGGAQKITVRHQER
                     TEEIEARWIVDASGVAAILARQEGWLRPNLEHPTSAAWSRWKGVKDWEGFDLAKKYPC
                     WSKACYGLRGTATNHLMGDGWWAWMITLKGGDVSIGVVFDQRRVQWPEGGTMAQRLKD
                     FLMRHPIGRELMADAQWTDGDVHWRKNLPYYSTTYAGDGFSLVGDAAAFLDPFYSPGM
                     DWITFTATCTAEMILMQQRGELKPERIERHNRDFIRSYERWFQAIYKDKYEYMGDFQL
                     LKLAFLMDLGLYYVGVASQPFKRGVVGLTEPLYSTPPSVPFFYWMRAYNRRFAKIAQS
                     RRARNCLGVKNYKQRYFLKGYTFSPSSLNPVWSSLLQWAWIELTEGWRTWFTKREIQK
                     EVPAMQPKVAMKPMP"
     gene            complement(2322..>2936)
                     /locus_tag="VG754_03540"
     CDS             complement(2322..>2936)
                     /locus_tag="VG754_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HWF18310.1"
                     /translation="EPFVHAKFTTNNSPIYYDNDLEFFIAGPDAYYEFEMNAFNTTYE
                     VFFIWDEAYERDSFSQSQEFLRTNLVPFDGVGFTNHPRGGRLGNFSWHFPGKQTAVFV
                     DGTVNNDEDHDRGWSVELAFPWKGMKWLAKGDNRSLPPKDGDVWRMDFSRFNTYKEAP
                     PAKDSGGWVWSRHGVWDSHIPECFPYIKFSTNDVSHAASERHSD"
BASE COUNT          698 a          753 c          831 g          654 t
ORIGIN      
        1 atggccttgt gttaccattt cgcatgatta aacctacaat acatcataaa actatcatgc
       61 ttgcgctgct gttggccggg ttgagctggc cggcttttgc gcgtgaaatg acggcttacc
      121 agttggtcaa ggaagccgac cgttatgtcg gcgaggacgt gaaagaccag gtggtcgagc
      181 tacgctccga gaaatcaatc ggaggattgg tccccaatat ctggttcgtt accttttatg
      241 atcccgacgc gacattcaaa gccaccgaaa tcaagttcgg cgccggcaag aaactcgaag
      301 tcaaacgtcc tgcccgcgtg ttggaatgga acaccaagga ttcgcaaaag ctggatccca
      361 aaaaattgaa tgtggattca gacaaggcga tttccatcgc gaccaaggag ccggtcttga
      421 aaggtttgaa tcttaaggcc acgcaacttt ggctggagca cagcagtgac ggaccggtct
      481 ggaaggtgcg catttgggcc gcgaagttgc gaaatccgaa cgatgacgcc aacctcggcg
      541 aagtctatgt caaggcggac gatggcacgg tcacgaagga tgatctgcac attgaccgcg
      601 tcgattgatc aggacggcgg ttttagggaa acacgaccga ttggccttca ttacacggct
      661 gaagacctga ttctcagctt gaatacaacg ggacattatt catgaacaag gacaatccgg
      721 cggaaacaac atttgacgtg gtcatcgtgg gcggcgcgct gtccggcgcg gcaacggcgg
      781 ttttgctgtt gaaagaaaaa ccggatttgc gcgtccttat agtcgagaaa tccaccgctt
      841 tcacgcgcag ggtcggcgaa gcgacgattg aagtcagcac ttatttcctg acgcattcat
      901 tgggattgac gggtcatctc accgaatcac acctgtcaaa acaaggattg cgcttctggt
      961 tcgcaaacga aaaaacccaa acgctcggcg attgcagcga gatcggcgga cgttacctcg
     1021 cgcgcgtgcc ggcttttctc gttgaccgcg ccgtgatgga ccaggaggtc ttgcgccgcg
     1081 cgtgcgcgat gggcgcgcaa ttgtggcgtc cggcaaccgt ccaaaaaatc gaactcgttc
     1141 tcggcggcgc tcaaaaaatc accgttcgtc atcaggaacg aacggaagaa atcgaagcgc
     1201 gctggattgt ggatgcatcg ggcgtggcgg ccatccttgc gcgtcaggag ggttggttgc
     1261 gtccgaatct tgaacacccg acgtccgcgg cctggtcgcg ttggaagggc gtgaaggatt
     1321 gggaaggttt tgacctcgca aaaaaatatc cgtgctggtc aaaagcttgt tacggcttgc
     1381 gcggaacggc gacgaaccat ttgatgggcg acggttggtg ggcatggatg attacgctca
     1441 aaggcggcga cgtgagtatc ggcgtggtgt tcgaccagcg gcgcgtgcag tggccggaag
     1501 gcggcacgat ggcgcagcgg ttgaaggatt ttttgatgcg acatcccatc gggcgcgaat
     1561 tgatggccga cgctcaatgg accgacggcg atgtgcattg gcgcaagaac ctgccttatt
     1621 attcgacgac ttatgcgggt gatggatttt cgctggtcgg cgatgcggct gcttttcttg
     1681 atccatttta cagtcccggc atggattgga tcacattcac ggcgacctgc acggctgaaa
     1741 tgatccttat gcaacaacgc ggagaattga aaccggagcg catcgagcgc cacaaccgcg
     1801 atttcatccg cagttacgag cgctggttcc aggcaattta taaggacaag tacgaataca
     1861 tgggagactt ccaattgctg aagctggcgt tcctgatgga cctggggctt tactacgtcg
     1921 gcgtggcgtc gcagcctttc aagcgcggcg tggtgggact gaccgaaccg ctctattcaa
     1981 ccccgccatc ggtgccgttt ttttattgga tgcgcgcgta caaccggcgg ttcgcaaaaa
     2041 tcgcgcaatc acggcgcgcg cggaattgcc ttggcgtcaa aaattacaag cagcggtatt
     2101 ttcttaaagg ctacactttt tcgcccagca gcctgaaccc cgtctggtca tctttgctgc
     2161 aatgggcttg gattgaactc accgaaggct ggcgcacatg gttcacgaag cgcgagattc
     2221 agaaggaagt ccccgccatg caacccaaag ttgcgatgaa gccaatgcca taggaaagca
     2281 tcaaattcca agctcgaatc agggaaaagc ttgtctttaa attaatcaga atgccgctcg
     2341 cttgccgcgt ggctcacatc attcgttgaa aatttgatgt acggaaaaca ttccggaatg
     2401 tgtgaatccc aaacaccatg gcggctccag acccatccgc cggaatcttt agcaggcggc
     2461 gcttccttgt aggtattgaa ccgcgaaaaa tccatgcgcc agacatcgcc gtctttcggc
     2521 ggcagcgaac gattgtcccc tttcgcaagc catttcattc ctttccacgg aaacgccagt
     2581 tcaacgctcc agccacggtc atggtcctca tcgttgttga ccgtgccgtc aacgaacacc
     2641 gcagtttgct tgccgggaaa atgccagcta aagttgccga gccgtccgcc acgcggatgg
     2701 ttcgtgaagc cgacgccatc aaatggaacc aagtttgttc gtaaaaattc ctgtgattgc
     2761 gagaagctgt cacgttcgta agcctcgtcc caaatgaaaa aaacctcgta agtcgtgttg
     2821 aacgcgttca tctcgaactc ataatacgca tctggtccgg cgatgaagaa ttcgagatcg
     2881 ttgtcgtaat agatgggcga gttgttggtg gtgaacttgg cgtgaacgaa cggttc
//
LOCUS       DASZMM010000141         8705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1963135, whole genome shotgun sequence.
ACCESSION   DASZMM010000141 DASZMM010000000
VERSION     DASZMM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963135"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VG754_03545"
     CDS             complement(<1..489)
                     /locus_tag="VG754_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18311.1"
                     /translation="MNTSGINHFRTPKKQAFTLIELLVVIAIIAILAGLLLPALAKAK
                     IKAQGIQCMNNNKQLMLAALVYPDDFNGVWIPNEPQGTGGQTDWVTLGMDWNSGNADN
                     TNFQKIIDKTYCVLAPYIGGNYKIFHCPGDKSHVVNEGDRVRSVSASQAVGSVWVTIG
                     CCL"
     gene            861..7292
                     /locus_tag="VG754_03550"
     CDS             861..7292
                     /locus_tag="VG754_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HWF18312.1"
                     /translation="MHKFRPREQFAGMMSANCSRSLVWGLFVVLFASASPAFAQTAFL
                     SFNTVGQYTNNFSPWNDNGAGVNNGNYSFAENASDGVGGSGGVSVFTNNDMTATYKNQ
                     SWNFATNGATLIVSTLVVANGQTSGNKVQLGVINSTTNGFNNNAGVQFESFRFTPASA
                     TSWTLYEQYRSSNVLTTSGALGTVSVTSGHWYKFAVGLTNTSGASGNINAGCALFDYG
                     TNGLVPGTNVIGFTTTQTHAGLNIATNTAVWPGLRAYQNAGISAWDNFLVFTSNSAPV
                     FTLTFSNMIVPLGNPITFSVLADGPGAITYSWYTNGALVSGLTGSNYTTTPTSSALTN
                     VTVVATNSNGSITNRATINAAAIMTPIALTGFNRDVVIENSASGPPYTAYALEFNLGE
                     GTCYYQNGLTGTTYGLPASGSFNSVIDQTVFQFQPYTASNALVMSADTSITNGTLALV
                     NPATYSTISLIANSGNATPTSTGVLTLNFADGSTYVTNYDAADWFFNSGFALQGVDRI
                     NISSGAPDNSSTDPRFYQTTLDLAAILGSANQTLVSLTFGQAPNSGSTGVYAVSGSAT
                     NNSFTLASITNSVATGIEASSATLAGTVLSSGSHAPQVTIYFGTNDAGTNALAWQAST
                     FLGFQSGNFSQTVGNLQPGATYYFTARAVNSAGISWATPSQVFTTLAAAQAEIANVPA
                     ANITANTATLAANVLNTGGNTPVVTIFYGPADGGSVASNWAGSFNLGLESGYCAVTAP
                     GLSPSTKYYFAAQATNSGGIVWASPSQSFTTLATNPVSTLVSVLTFHNNNTRNGQNTN
                     ETQLTLSNVNTNTFGKLFSHSVDGYVFAQPLIATNVVIPGKGLHNVVYVVTENDSIYA
                     FDADSNQGPNANPLWQVSFINPSAGVTTLSSSDAGTSDITPEVGSTATPVIDPSTGTL
                     YLEVKTKEVSGNVTSYVHRLHALDITTGAERASGVVSNSPVVIAATNYPGNGDGNADD
                     TDGSGHVLWNGLREHSRPALTLLNGVLYIAYASHGDNNPYHGWLFTYDAHSLAQRNVY
                     NTTPNGLRGGIWQGGGGATVDPAGNLYYITGNGNSSTDFNATGATFSTSNNFAMSVLK
                     FVPSNGIPVLTDFFTPYNQASLTGGDVDFGSGAALVLPDSVGNTNHQHLLAAAGKGGK
                     IYLIDRDKMGHFNAPGDTQIVQVLTNALGTAPQNGSYMTPAFFNNTLYYIGMNSTLMA
                     FSISNASINSTPVQTTTVYGDKGSSSPSVSANGTSNGIVWATESDAYASSGPGILHAY
                     NAANVAQELYNSSQLLSRDNPGGAVKFSVPTIANGKVYMGTEYNLSVFGLGAFLPAPV
                     IQPNGGMFTNSVTISFSDSKAGASFYYTLDGSIPTINSIPYSGAFVLTNTAIVQVIAA
                     AAGGVSPVTSADFINSAAAGNGIGLTGSYYSNQLATFNPPASLVRTDAAVNFNWQSAP
                     PAPSVGATNFSVRWTGCVQPQYGETYTFYTYTDAGVRLWINGQLLIDHWSNQPPTQWS
                     ASIPMVAQQLYNIQMDYYYQNQGTPLAQLSWSSPSTPTEIVPQTQLYPFTNPPPAIVM
                     TSPTNGATFTAAASVTLGADAAAQSNAITQVVFVINGQPFGAVTTPPYAVTATGLAAG
                     NYTLTAVASDTTGLSSTSAPVNVTVVAGSGAAYGMTNYPVSPAFYNMPTVFTGSLPSR
                     LSLTGVFTNTPNMTPVASLIPYSPNVPLWSDGALKVRYLSVSNSGTPLVPGEQIAFAP
                     TGTWSFPSGTVFVKTFELLTNQSDPNSIHRLETRLLVRDTNGAVYGVTYKWRADNSDA
                     DLLASNLTENISITTPNGVITQSWYYPSPGDCLQCHTPVANYVLGVSARQLNGNLTYS
                     NGVTDNQLRALNRAGLFYPAIDESSISNIETLFSLTNQSASFEQRSRSYLDANCAQCH
                     QPGGTGPTFDARFDTPLTNQNIIYGVLAKGNLGYDNAYVVTPKDTWRSILYDRIDSTD
                     SAVKMPPLARNLVDTNAVAIIAEWINSLPGTPALPPPTITPASGLYSGPVNVTLQSQT
                     NNTALYYTLDGSLPTTNSFLYTGPFTVSSNETVSASAWESGYVNSVATTASFNIIGIF
                     FAPQAGFTNGAFSMSFWGPTGYHYVLQTSTNLTQWIPISTNYPSTIPFVLTDPSAINS
                     PARFYRVLQQP"
     gene            7289..7840
                     /locus_tag="VG754_03555"
     CDS             7289..7840
                     /locus_tag="VG754_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18313.1"
                     /translation="MNHRPLFQKGLAGLLMLMLLIVQASALAQTNSAQSDQSVASNAP
                     TVTNLVRAQQIRAACIQGRRYICGRVLEVTRNGLVVDSGYTSLLQPPFNHSWVVAGNV
                     NATRPSNILEGTAPDSPCVGLVFLTDIPKRPAVHQYDYIVIHGFPAGSYNYKPLAGIS
                     KTVRRFSAGLETAVELNLNVKEH"
     gene            7824..>8705
                     /locus_tag="VG754_03560"
     CDS             7824..>8705
                     /locus_tag="VG754_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18314.1"
                     /translation="MSKNIESQKRFFGKIFRALFCASMAMAIIGGVISCRSGSAKTTN
                     RNAVDWKPSSGTALNMPDSSKGEIPKLLSQTGAFKNIRDLIPNDRLVPYDLNVPFWSD
                     GAEKLRWMAPPDWHESAPQKIQFTPTGEWTFPGGTIFVKHFELATNELHPELKRRLET
                     RLIVRTSSGGVYGVTYKWRPDNSDADLLATNLSEPLLITTATGTRTQYWYYPSREDCL
                     VCHTANAGLVLGVKTRQLNRNFTYPSGVTDNELRVWNQLGLFDTNLDKADFANLPKLA
                     RADDMSRTLEDRARSYLD"
BASE COUNT         1985 a         2564 c         2252 g         1904 t
ORIGIN      
        1 aagacagcat ccgatagtca cccaaaccga accaaccgcc tggcttgcgg aaacgctgcg
       61 cacccggtct ccctcgttta ccacgtggct cttatctccg ggacaatgaa atatcttgta
      121 gtttccaccg atgtagggag cgaggacaca ataggtcttg tctatgatct tttgaaaatt
      181 agtgttatct gcgttgccgg aattccaatc catcccgagc gtgacccaat cggtttggcc
      241 acccgttcct tgtggctcat tggggatcca aacaccattg aaatcatcag ggtaaaccag
      301 cgcggccagc atgagctgtt tgttgttgtt catgcattgg atgccttggg cctttatttt
      361 cgctttggcg agcgcgggca gcaacaagcc ggcgaggatt gcaataatgg cgattacgac
      421 caacagttca atcagcgtaa acgcctgttt ttttggggtg cgaaaatggt tgatgccgga
      481 cgtgttcatg ggatttctgt tcagtacttt atagcttcat tcggacaaac gcaagctttt
      541 tgaaaccggg gacaaacccg gttcgttgga cggacggtgc tgccgcactt aaccacaggg
      601 aacatcttgg tgcaattaag caaggcacta aaaaggtgtc aacgggtatt ttcctctatg
      661 tcgaacgaat ttgcgcctat ttccgttata tcgaacagat ttaggcccaa aatagcgtta
      721 tttgcactga catgtcggat ttcccgacaa gtggatgttc ggccttgtat ttcagtgttc
      781 tcatcaccaa tcgccatgat tgcaatccga attacaaatg cagactcgac ttcaacccaa
      841 agtttgataa cgtcaccgac atgcacaaat tcaggcctcg cgagcaattc gctggcatga
      901 tgtcagccaa ctgctcccgg tcgttggttt ggggcttgtt cgtcgtcctc tttgcaagtg
      961 cgtcgccagc cttcgctcaa acagcttttc tttccttcaa cacggtcgga caatatacaa
     1021 acaacttcag tccatggaac gataacgggg cgggcgtcaa taacggcaat tattcttttg
     1081 ccgaaaatgc aagcgatggc gtcggcggca gcggcggggt gagcgttttc accaataatg
     1141 acatgacggc cacttacaaa aaccagagct ggaattttgc cacaaacggc gcgacgctca
     1201 tagtctcgac tctcgttgtt gcgaatggcc aaaccagcgg caacaaggtt cagttggggg
     1261 tcatcaattc cacgaccaac ggattcaaca acaacgccgg ggtacaattt gagagtttcc
     1321 gtttcactcc cgccagcgcc accagttgga cactttacga gcaatatcgg agcagcaacg
     1381 tcctgaccac cagcggcgcg ttgggcacag tttcagtgac ctcggggcac tggtataaat
     1441 tcgcggtggg actcacaaac acttccgggg cttcgggaaa catcaatgcc ggctgcgccc
     1501 ttttcgacta tggaacaaac ggattggttc ccggcacgaa cgtgatcggc ttcacaacca
     1561 cgcaaaccca tgccgggttg aacatcgcca cgaacaccgc cgtttggcca ggactgcgcg
     1621 cttatcaaaa tgcgggcatc agcgcgtggg acaatttcct ggtgttcaca agcaacagcg
     1681 cgccagtgtt cacgcttaca ttcagcaaca tgatcgtgcc attgggaaac ccgatcacgt
     1741 tttctgtgct ggccgatggc cccggcgcaa tcacttacag ttggtacacg aatggcgcgc
     1801 tcgtttcggg tctcacaggc tcgaattaca cgactacgcc tacaagctcc gcgctcacaa
     1861 atgtgaccgt ggtcgccact aattccaatg gctccatcac aaaccgggcc acaatcaatg
     1921 cggcggccat tatgacaccc atcgcactca ccgggttcaa tcgtgacgtg gtgattgaaa
     1981 acagcgcgtc gggaccgcct tacacggcgt acgcactgga attcaacctg ggcgaaggga
     2041 cttgctatta tcaaaacggg ctgaccggca caacctacgg attgccggca tccggctcct
     2101 tcaacagcgt gattgaccag accgtgttcc aattccagcc ctataccgcg agcaacgctt
     2161 tggtgatgag cgccgacaca agcatcacga atggaacact ggcgctggtg aatcccgcca
     2221 cttacagcac tatttcgctc attgccaatt cgggaaatgc gacccccacg agcaccggtg
     2281 tgttgacgtt gaattttgcc gatggcagca cttacgtgac gaattatgat gcggcggatt
     2341 ggtttttcaa ttctggcttc gcgttgcaag gagttgaccg gatcaacatc agctctggcg
     2401 cgccggacaa cagctcgacc gatccgcgtt tttatcaaac gacccttgat ctcgcagcaa
     2461 ttcttggttc ggccaaccag actttggtga gcctgacgtt cggacaagcg ccgaattccg
     2521 gttccacggg tgtttacgcc gtgagcggtt cggccacgaa caatagtttt acgttggcct
     2581 caatcacaaa ttctgtggcg actggcatcg aagccagttc agccactctg gccggaacag
     2641 tcctttccag cgggagccac gctccacagg taaccattta ctttggaacc aatgacgccg
     2701 gcacgaatgc cttggcatgg caggcgagca cttttttggg ttttcaaagc ggtaacttca
     2761 gccagacagt tggcaatctg caacccggcg cgacatatta ttttacagca cgcgctgtga
     2821 acagcgccgg aatttcctgg gccacgccat cgcaggtatt caccacgctc gcagccgcgc
     2881 aagcagaaat agccaatgtg cctgccgcca acatcactgc caatacggcg acgctggccg
     2941 cgaatgtttt gaacaccggc gggaacacgc ccgtggtcac cattttttat ggtccggctg
     3001 atggtgggtc cgtcgccagc aactgggctg gcagtttcaa tctcggattg gaatcaggtt
     3061 attgcgcggt aacggcgccc ggattgtcgc cgagcacaaa atattatttt gctgcgcaag
     3121 ccacgaactc cggtggaatc gtttgggctt ccccctcgca atcgttcacc acgctcgcga
     3181 cgaatcccgt ttccactttg gtttcggtgc tcacgtttca caacaacaac acgcgcaacg
     3241 gtcagaacac taatgaaact caattgacgc tctccaacgt caatacgaac acattcggaa
     3301 aactattcag ccacagtgtg gatggatacg tttttgcgca gccgctgatc gcaaccaatg
     3361 tggtgattcc cggcaaggga ttgcacaatg tggtctatgt ggtgacggaa aatgacagca
     3421 tttacgcatt cgatgccgac agtaatcaag gtccaaacgc caatccgctt tggcaggtga
     3481 gtttcatcaa tccatcagca ggagtgacca cgctgtcgag ttcggacgcg ggaacgtcgg
     3541 acatcacgcc ggaagtcggc agcacagcca cgccggtaat tgatccctca accggaacgc
     3601 tctatctgga agtcaagacg aaggaggtca gcggcaatgt cacgagttac gtgcaccggc
     3661 ttcatgcgct cgacatcacg acaggtgcgg aacgagccag tggcgtggtt tccaacagcc
     3721 cggtggtgat cgcggcaacg aactatcccg gcaacggcga tggcaatgcg gatgatacgg
     3781 atggaagcgg ccatgtgctc tggaacggct tgcgcgaaca cagccgaccc gcgctgaccc
     3841 tgttgaatgg agttctctat atcgcctatg cgtcccatgg ggacaacaat ccttatcatg
     3901 gatggttgtt cacatacgac gcgcacagcc ttgctcaacg gaatgtttat aatactacgc
     3961 cgaacggact gcgcggcgga atctggcagg gcggcggcgg cgcaacggtg gatccggcag
     4021 gcaatcttta ttacatcacc ggcaacggga acagctcgac ggatttcaat gcgaccggcg
     4081 cgacattcag cacttcaaac aattttgcga tgagcgtttt gaaatttgtg ccgagcaatg
     4141 gcataccagt gctgaccgat ttcttcacgc catacaatca ggcttcattg accggcggcg
     4201 atgttgattt cggctcaggc gccgcgctgg tgttgcccga ttcggtcggc aacacgaacc
     4261 atcagcatct tctcgctgcc gccggcaagg gcggaaagat atatctgatt gatcgcgaca
     4321 agatgggtca cttcaacgcg cccggcgata ctcaaatcgt ccaagtgctg accaacgcgc
     4381 tcggaacggc cccgcaaaat ggcagttaca tgacgcctgc ctttttcaac aacacgcttt
     4441 attacatcgg catgaacagc acattgatgg cattttccat cagcaacgca tccatcaaca
     4501 gcacgcctgt tcaaaccacc acggtttatg gcgataaggg cagcagttca ccttccgttt
     4561 cggccaacgg cacgagcaac ggcatcgtgt gggcgacgga atcggatgcg tacgccagtt
     4621 ccgggccggg cattttgcac gcatataatg ccgcgaatgt cgcgcaggaa ctttacaaca
     4681 gcagtcagtt gttgtcccgc gataatccag gcggcgcggt gaaattcagc gtcccgacta
     4741 tcgccaacgg caaggtttac atgggcacgg aatacaattt gtcggtgttc ggacttggcg
     4801 catttctgcc tgcgccggtc atccagccga acggcggaat gttcaccaat tccgtgacga
     4861 tttcgttttc tgattcgaag gcgggcgctt cgttttatta cacgctggac ggcagcattc
     4921 cgaccatcaa ctccatcccc tactccggcg cgtttgtgtt gaccaacaca gcgatagtcc
     4981 aggtgattgc cgcggcggcg ggaggagtaa gccccgtcac aagcgccgac ttcatcaaca
     5041 gcgccgctgc gggaaatggc atcggtctga ccggctcgta ttactcgaac caactggcca
     5101 ccttcaatcc gccggcttca ttggttcgca ctgacgcggc cgtcaatttc aactggcaaa
     5161 gcgcgccgcc cgctccgtct gtcggcgcca cgaatttcag cgtgcgttgg accggctgcg
     5221 tgcagccgca atacggcgag acttacacgt tctacactta caccgacgcc ggcgtgcgct
     5281 tgtggatcaa cgggcaactg ttgattgacc attggagcaa ccagccgcca acgcaatgga
     5341 gcgcttcgat accgatggtc gcgcagcagc tctacaacat ccaaatggat tattattacc
     5401 aaaaccaagg aacgccgctc gcgcaacttt cctggagcag tccgtcaacg ccgacagaaa
     5461 tcgtcccgca aacgcaactt tatcccttca ccaatccacc accggcgatt gtgatgactt
     5521 cacccactaa cggcgcgacg ttcacggcgg cagccagcgt cacgctggga gcggacgccg
     5581 ctgctcaatc aaatgcgata acgcaagtcg tcttcgtgat caacggacaa ccattcggcg
     5641 cggtgacaac tccgccttac gcggtcactg cgacaggatt ggcggccgga aattacacgc
     5701 tcaccgcagt cgcgtcggac accaccggtt tgtcgagcac atccgcgccg gtcaatgtca
     5761 ccgttgtggc aggaagcgga gcggcttacg gaatgaccaa ttatcccgtg tcgcccgcgt
     5821 tctacaacat gcccacggtg ttcaccggct cgttgccttc gagattgtct ctgacgggcg
     5881 tcttcaccaa cacgcccaac atgacgcccg tcgcgtcact gatcccgtat tcgccgaatg
     5941 tcccgctctg gtcggatggc gcgctgaagg tgcgctatct ttcagtgtcg aattccggaa
     6001 cgcctctcgt tcccggcgaa cagatcgctt ttgcgccgac tggcacatgg tcattcccgt
     6061 cgggcacagt gttcgtcaaa acatttgagc tgctcaccaa ccagagcgat cccaattcaa
     6121 tccaccgttt ggaaacgcga ttgctggtgc gcgacaccaa cggagcagtc tatggcgtca
     6181 cttacaaatg gcgcgccgat aacagcgatg ccgatttgct cgcgagcaac ttgacggaaa
     6241 acatttccat caccactccg aacggcgtca tcacgcaatc gtggtactat ccaagccccg
     6301 gtgattgcct gcaatgccat acgccagttg ccaattacgt gctcggcgtc agcgcgcgcc
     6361 agttgaacgg aaacctgacc tattcgaacg gcgtgacgga caatcaactg cgggcgctca
     6421 atcgcgccgg tttattttat cccgcgattg acgaatcaag catcagcaac atcgaaacgc
     6481 tgttttcatt gacgaaccag tccgcatcgt tcgaacaacg ctcgcgttcg tatctcgatg
     6541 cgaattgcgc gcaatgccat caaccgggtg gaaccgggcc gacctttgac gcccgtttcg
     6601 acacgccgct gacgaaccag aacatcattt acggcgttct ggccaagggc aatctcggct
     6661 acgataacgc ctacgtcgtc acaccgaaag atacctggcg ttcaatcctc tacgaccgga
     6721 tcgactcgac ggattcggcg gtcaagatgc cgccgctggc gcggaatttg gtggatacca
     6781 acgctgtcgc gatcatcgcc gaatggatca acagcctgcc cggcacgccc gctttgccgc
     6841 cgccgaccat cacacccgca agcggccttt attccgggcc tgtcaacgtg accttgcaat
     6901 cgcagaccaa caacacggcg ctgtattaca ccctggacgg ttcattgccg acgaccaact
     6961 cattccttta caccgggccg ttcaccgtct cgtccaatga aacagtcagc gccagcgcgt
     7021 gggaatccgg ttacgtcaac agcgtggcaa cgaccgcctc gttcaacatc atcggaattt
     7081 tcttcgcgcc gcaagcgggc ttcacgaacg gcgcgttctc gatgtcgttc tgggggccga
     7141 ccgggtatca ttacgttttg cagaccagca ccaatctcac ccaatggatt cccatcagca
     7201 cgaattatcc atccaccatt ccctttgtcc tgacggaccc atcggcgatc aacagcccgg
     7261 cgcggttcta ccgagtcctg caacaaccat gaaccaccgg ccgctttttc aaaagggcct
     7321 tgcgggtttg ttgatgttga tgttgctcat cgtgcaagcc tcggcgcttg ctcaaactaa
     7381 ttccgcgcaa tccgaccaat ctgttgcgtc gaacgcgcca accgtcacaa acttggtgcg
     7441 ggcacaacag attcgcgccg cgtgcatcca gggacgccga tacatttgcg gacgcgtgct
     7501 ggaggtgacg cgcaacggac tcgtggttga cagcggttac acgagtttgc tgcaaccgcc
     7561 cttcaaccat tcgtgggtgg ttgcggggaa cgtcaatgcc acccgccctt cgaatattct
     7621 cgaaggcacc gcgccggact ccccttgtgt cggtctggtt tttctcaccg atattccgaa
     7681 gcggcccgcc gtccaccaat atgattatat tgtgattcac ggtttcccgg cgggcagtta
     7741 caattacaaa ccgttggctg gcatctcgaa aaccgtccgg cgattttccg ccggcctcga
     7801 gacagccgtc gaactgaatt taaatgtcaa agaacattga gtcccaaaaa cgatttttcg
     7861 ggaaaatttt tcgcgcgctg ttttgcgctt ccatggcgat ggccatcatt ggcggcgtga
     7921 tttcctgccg ctcgggttcg gcgaaaacta cgaaccgaaa cgcggtggat tggaaacctt
     7981 cgagcggcac cgccttgaac atgccggact cgtcgaaagg tgaaattccc aagctgcttt
     8041 cgcaaaccgg cgctttcaag aacatccgcg acctgatccc gaacgaccgc ctggttccat
     8101 acgatttgaa cgtgccgttc tggtcggatg gcgcggagaa attgcgctgg atggcgccgc
     8161 ccgattggca cgaaagcgcg cctcaaaaaa ttcaattcac gcccaccggc gagtggacat
     8221 ttcccggcgg aacaattttc gtgaaacatt ttgaattggc cacgaacgaa ttgcatcccg
     8281 aactcaagcg gcgattggaa acgcggctga ttgtgcggac ttcctccggc ggcgtttacg
     8341 gagtgactta caaatggcgt ccggacaaca gcgacgccga cttgctggcc acgaatttgt
     8401 ccgaacccct cttgatcacc acggcgacag gcacccgaac gcaatactgg tattatccga
     8461 gccgcgagga ttgcctggtg tgtcacaccg ccaacgctgg tttggtgctc ggcgtcaaga
     8521 cgcgccaact gaaccggaat ttcacttatc cgtccggcgt cactgataac gaattgcgcg
     8581 tgtggaacca gcttggattg ttcgacacca atctcgacaa agcggatttc gcaaatctcc
     8641 cgaaactcgc ccgcgctgac gacatgtcgc gcactctgga agaccgcgcg cgttcctatc
     8701 tcgac
//
LOCUS       DASZMM010000142        10447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4187039, whole genome shotgun sequence.
ACCESSION   DASZMM010000142 DASZMM010000000
VERSION     DASZMM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4187039"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VG754_03565"
     CDS             complement(<1..574)
                     /locus_tag="VG754_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF18315.1"
                     /translation="MEAVAQSDLAEIAAVADAAPEAAANAATQFPGAVAVKSVEEILE
                     LELDGVVIATPSALHAEQAILALERGMAVFCQKPLARNESETRRVIDAARAADRLLAV
                     DLSYRFMSQVQKVRGLVCEGELGNVYAADLVFHNAYGPDKPWFYDRHLSGGGCVIDLG
                     IHLVDLALWMLDFPRVTNVTSRLFSQGKPLR"
     gene            complement(682..1635)
                     /locus_tag="VG754_03570"
     CDS             complement(682..1635)
                     /locus_tag="VG754_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWF18316.1"
                     /translation="MRAAVLENPGRVSLQRVKLPEPAEGWVRVKLEGCGVCGSNLPPW
                     EGRPWFKYPLAPGELGHEGWGRVDALGPKTSRFKIGERVTMLSYHAYAEYDIADENDL
                     VRLPEELDARPFPGEPLACAMNIFQRSEIQPGQTVAVVGIGFLGALLTSLCSQAGAQV
                     IAISRRPFALEMARRCGAAETIVLKDCKYIQDHVRKLTNGRGCERVIEAVGLQEALDL
                     ATELTRERGRLVIAGYHQDGLRQINMQLWNWRGLDVINAHERDRRNYVTGMEAAVRAV
                     ESGQLDPSFLYTHQFSLEQLGEAFDAMRDRPDQFLKGLIVL"
     gene            complement(1703..2821)
                     /locus_tag="VG754_03575"
     CDS             complement(1703..2821)
                     /locus_tag="VG754_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF18317.1"
                     /translation="MEKKVLITGGAGFIGSHLSDELLAHGYEVNVFDNLDPQVHGPRC
                     RRPSYLDPDVELIRGDVRDREKLQRALRGVDMVVHFAALVGVGQSMYQVADYIHVNNG
                     GTANLLQILIENPVERLVVASSMSLYGEGLYRAADGTLVSGIERSLAQLQSHDWELRG
                     VNGEILVPMPTPETKPPAIASAYALSKFDQEQLCLMLGRAYRIPTVALRFFNVYGPRQ
                     SLSNPYTGVLAIFASRLLNNNAPLIFEDGLQKRDFVSVHDIARAARLALETPGAAGQV
                     FNIGSGRHFTIREIADRMCRVMGKDEIEPEITGKYRVGDIRHCFADVRQARAILGYEP
                     LVSFDEGMLELADWLEGQVAEDRMEQARAELLARGLTV"
     gene            3041..4336
                     /locus_tag="VG754_03580"
     CDS             3041..4336
                     /locus_tag="VG754_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWF18318.1"
                     /translation="MEINPNFKHNGDGNGNSRKVRVAIIGVGNCASSLVQGVEFYRDA
                     REDEAVPGLMHVNLGGYHIRDIEFSAAFDINESKVGRDLSEAIFSAPNNTIKFSSVPH
                     KGLTVHRGMTHDGLGKYLSRVITKAPGSTDDIVGILRSTRTDVVVSYLPVGSEMATKW
                     YVEQVLEAGCAFINCIPVFIASQPYWQNRFAERGLPIIGDDIKSQVGATITHRVLTNL
                     FRERGVRLDHTYQLNFGGNTDFMNMLERERLESKKISKTNAVTSQLAVPLSEENAHVG
                     PSDYVPWLTDRKFCYIRMEGTTFGNVPLSCELKLEVWDSPNSAGVVIDAVRCAKLALD
                     RGIGGALIGPASYFMKSPPQQFTDSEARERTEAFISENRRNDDRSHQVVERAEQSVAL
                     GFSDGPGLETVRGNDAHGRHHHTATLPETAVDSRRDFPH"
     gene            4227..5102
                     /locus_tag="VG754_03585"
     CDS             4227..5102
                     /locus_tag="VG754_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HWF18319.1"
                     /translation="MDPGWKQFVEMTRMAAITIRRLCPKLPLILGGISPIDPDFIALL
                     DAQGLLDHLDGVAVHGFPLDWNHWQIDEWPNKLDEIRAVTSLPIWVTEVGVSTFGADE
                     VQVFGLQRTTELLHGRAERIFWYSLLDLPRKWEATTRHKESEGSAYYRHFYMGLIRED
                     GTPKPAVKHFNPELGICQWFHFQDHRLDFAVGWLRKLGVKKLRTGISWADWCRPGAIE
                     WYDRQMDALKDFDTTITLCFTPPSRGKAPNCTSPPVEPAEFAWFAREVVQRYVLSPET
                     GATNDYVLSDSPCVE"
     gene            5065..5661
                     /locus_tag="VG754_03590"
     CDS             5065..5661
                     /locus_tag="VG754_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWF18320.1"
                     /translation="MTTFYLIRHASNDVLGKAITGRKPGVSLNPQGLCEAENLAARLA
                     GKPIQMIFSSPLERVVETAVPLAKRLGLDIQISEALNEIDFGNWTHQTFEHLAELPGW
                     QRWNSFRSGTRAPNGETMLEAQTRIVTEIQRLRAKFPEQTLAFFSHGDLLRAALAYFL
                     GIPLDLFQRMEVSPASVSILQLNEHEPKVVCVNAQGSL"
     gene            complement(5609..6988)
                     /locus_tag="VG754_03595"
     CDS             complement(5609..6988)
                     /locus_tag="VG754_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18321.1"
                     /translation="MNHLGDRVLIVGIRSIGTALSAVVSATLASAGWRPMRITVRPSG
                     PPFARVVELPDSVHGCARRALVVDEGPGISGSSMAAVAKALVEIGFEPEQISFLPGHG
                     GEPGTAASDEVRGWWKRVRRYVTPWEQLRWSGMGLPECLREESREIAAQEQFTTVEDL
                     SGGRWREKIYPKPANRPWAAISFERMKFLCTAKSDASILWKFVGLRKSVADKSSASLK
                     EHSDHVWTPKLLGWFRGFVALPWVEGRPLSVEDAQNPEIIRHLGRYISAVSKAPLSDA
                     QQATSFECLCEMLYWNTREALGGEMAEHALRFANKVRKPSICPAYGDGRLAPHEWRRT
                     GDGQILKLDPSGHDMDHTMIGRQPVYWDTAGTMVEWGFSSSKLLLSALQGAGISLRTD
                     VLDFYQMSYAAFRMGQASVCADACAADAEEFVRTRQAFENYREQLRELLKLTANLVHS
                     HKPPLAHVH"
     gene            7598..7876
                     /locus_tag="VG754_03600"
     CDS             7598..7876
                     /locus_tag="VG754_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18322.1"
                     /translation="MRGNLLVYWFSWVNTICWVVCFWWMHRISARQDAFMKALKDQGI
                     RIERLSKVEHDMLKEVHPQVGEIKQAVTEVVQSTREQQETLPQTKPAR"
     gene            7982..9853
                     /locus_tag="VG754_03605"
     CDS             7982..9853
                     /locus_tag="VG754_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWF18323.1"
                     /translation="MLFGRRLATGEDRQEEVGPLAAIPVLGLDALASAAYGPEAALTV
                     LLPLGLLAPLKIGPITIVIIVVLALVAFSYQQTISAYPNGGGSYTVAKENLGNRWGLL
                     AGAALTVDYILNVAVGISAGVAAIGSAFPPLLRYTLPLCLGLLAILTLINLRGVREAG
                     VAFMLPTYMFVGCLFVALGMGTLHVILHHGHPILVTPPSPLPPPREAITLWLLVRAFA
                     NGCTAMTGVEAVSNAVPIFREPKVKLAKLTLLTIIGILIVLLLGIAFVCRAYGIGATQ
                     PGSREYQSVLSELVGAVAGRGWFYYVSMASVFSVLALSANTSFAGFPRLCRLMALDQY
                     LPEAFATRGRRLVYTYGIALLAIFAFILLIIFRGITNGLIPLFAIGALLSFTLSQSGM
                     VAHWRRRLKDPHARLALCINGAGALATGITVLIVMVSKFVEGAWITILLMPIIMVALQ
                     RLQRHQRRLAAEVESHAPLDFCRVDAPIMVVPLTGWTRVAEKALVFATRISPEVHAVQ
                     VLTKEVPKEDLSEHWDELVEKPAQHAGVNPPILKVVRSKYRQELRPIVNFVRELVRKN
                     PDRTVGVVVPELVERRWYHYFFSYATLLRGMLLLRGNPRIVIMTMPWYPHGSEHGAA"
     gene            complement(9870..>10447)
                     /locus_tag="VG754_03610"
     CDS             complement(9870..>10447)
                     /locus_tag="VG754_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18324.1"
                     /translation="YRARFAYEHTPEFQAETLGRAVASTLATGKVQLVAWYRIHDLPA
                     NTQVIGDENNLRLGVLDESGRPKPALRALDFFHQLFAGGFCCLDSTTPEVRHIQSDSE
                     AHVFEKSDGEEFVTAWLRTVTLSPTFATSEDLRREKLTVVLADRCEQMSVYNELGFRE
                     SIARLRKAGKNRKVLNIPLHGGELKVYGCEP"
BASE COUNT         2394 a         2957 c         2760 g         2336 t
ORIGIN      
        1 tgcgaagcgg tttgccttgc gaaaagagcc ggctcgtgac attcgttacg cgaggaaaat
       61 ccaacatcca aagcgcgagg tccaccagat ggatgcccaa atcaatcaca caaccgccgc
      121 ctgaaagatg gcgatcgtaa aaccatggct tgtccggccc gtacgcgttg tgaaacacca
      181 aatcggcagc gtaaacattc ccaagctcgc cctcacacac gaggccacgc actttctgca
      241 cttgcgacat gaaacggtag gagagatcga cagccagcaa tcggtccgct gcacgtgccg
      301 catcaataac gcgacgtgtt tccgattcat ttcgtgcgag cggtttttga cagaagacgg
      361 ccatgccacg ctcgagcgcc aaaatcgcct gttcggcatg caaagcgctc ggcgtggcaa
      421 tgaccacccc gtccaattcc aattccaata tttcttcgac cgatttcaca gcaaccgctc
      481 cgggaaactg agttgcagca ttcgccgctg cttccggagc agcatcggca accgcagcaa
      541 tttccgccaa gtcactttgc gcgacagctt ccatccgatg ccgtccaatc caaccgacac
      601 ccaaaaaacc gagcagcggc ttcttcgcgg ctttggaaag aattggtgcg tgaaaggttt
      661 cgatggcaga agctgtcgtg ttcataagac gatcaaacct ttgagaaatt gatccggacg
      721 atcgcgcatt gcatcaaacg cctcaccgag ttgctcaagc gaaaactgat gcgtgtacag
      781 aaatgaagga tcgagttggc cggactcgac cgcgcgaacg gcagcctcca tcccggtgac
      841 ataattccgg cgatcgcgct cgtgggcatt gatcacgtcc agtccacgcc aattccatag
      901 ttgcatgttg atttggcgca agccgtcctg atgataacct gcgatgacca atcggccgcg
      961 ttcacgcgta agttccgtcg cgaggtctaa tgcttcttgc aggcccaccg cttcgatgac
     1021 gcgctcgcag ccgcggccat tcgtcaattt tcgaacgtgg tcttggatgt atttgcaatc
     1081 cttgagcaca atcgtttcag ccgcgccaca acgccgggcc atttccagcg cgaaaggccg
     1141 gcgagaaatc gcaatcacct gggcgcccgc ctgagaacat agcgaagtca acaaagcgcc
     1201 caaaaaacca atgcccacta ccgcgacggt ctgtcccggt tgaatctcag agcgttgaaa
     1261 aatattcatc gcgcatgcga gcggttcgcc cggaaacggt cgggcatcca actcttccgg
     1321 caaacggacc aagtcatttt catccgcgat gtcgtattcc gcatacgcgt ggtacgaaag
     1381 catcgtcact cgctccccga ttttgaaacg ggaagttttt ggcccaagcg cgtccacccg
     1441 accccatcct tcgtgtccga gttcgccggg cgcgagcgga tatttgaacc aaggacgacc
     1501 ttcccatggc ggcaaattgg aaccgcacac gccgcaacct tccaacttca cgcgcaccca
     1561 accttccgca ggttccggca gcttcactcg ttggaggctc actcgtcctg gattttccag
     1621 cactgctgcg cgcatggttg aggtttttcg cattttagtt cgcacgaact tgttcgactt
     1681 gccgaaccgc acaagaacat tctcaaacgg taagaccgcg ggccaagagt tcggcgcgtg
     1741 cctgctccat ccggtcctcc gccacctggc cttcgagcca gtcggccagt tccaacattc
     1801 cttcatcaaa ggagacgagc ggttcgtatc caagaatggc acgggcttgc ctgacgtccg
     1861 cgaagcaatg tcgaatgtcc cccacgcggt atttcccggt gatctcaggt tcaatctcgt
     1921 ccttgcccat cacgcggcac atccggtccg cgatttccct gatggtaaaa tgtctcccgc
     1981 tgccgatgtt gaacacctga ccggctgccc caggcgtttc cagagccaaa cgcgcggcac
     2041 gggcgatgtc atgcacactc acaaaatccc gcttttgcaa tccgtcctca aaaatcaaag
     2101 gcgcattgtt attgagcagc cgcgaagcaa agatggcgag cacaccggtg taagggttcg
     2161 atagggactg gcgcgggcca tacacgttga aaaacctcaa tgccacggtt ggaatccgat
     2221 acgcgcgacc cagcatgaga caaagttgtt cctggtcaaa cttcgaaagt gcataggccg
     2281 aagcaatggc gggcggcttg gtctccggtg tcggcatcgg cacaaggatt tctccattga
     2341 cgccgcgcaa ttcccaatcg tgcgattgca attgcgccag gcttcgctcg atgccggaca
     2401 ccagagttcc gtctgcggcg cggtacaggc cctcgccata aagactcatg ctggaagcca
     2461 cgacaagccg ctccaccggg ttctcaatta aaatttgcag aaggttggcc gtgccgccgt
     2521 tattgacatg aatataatcg gcaacttgat acatgctctg gccgacaccc actaaggccg
     2581 cgaaatgcac caccatgtcc accccgcgca atgcgcgctg caacttttct ctgtcgcgaa
     2641 cgtcgcctcg aatcaattcc acgtcaggat ccagataaga aggtcttcgg caacgcgggc
     2701 cgtgcacttg tgggtcgaga ttatcaaata catttacttc gtaaccgtgt gcgagcagtt
     2761 cgtccgagag atgcgatcct atgaatcctg cgccgccggt gatcagaact tttttctcca
     2821 tgttccttga aaatgaatca tctgccgacg tttacaactg gggcctatcc cgcgtttata
     2881 aatgaggaat tcctcatcgc aaatagtgtt caaatttttt ggaccaataa tcacattttt
     2941 cggaaaaaga aatcttgagg agcgaccgcc ggggctttcc gctgaaaagc atgtcaacac
     3001 ccaatggcag cgtacaaatt atgcgggcaa gttcgctctc atggagatca atccaaattt
     3061 taagcacaac ggtgatggca atggtaactc ccgcaaagtc cgcgtggcta tcatcggcgt
     3121 tggcaattgt gcttcatcgc tggtgcaggg agttgaattt tatcgcgacg ccagggagga
     3181 cgaggccgtg cccgggttga tgcatgtcaa cctgggcggc tatcacattc gcgacatcga
     3241 attctcagcg gcgttcgaca tcaacgaatc gaaggttggt cgcgatcttt ctgaagccat
     3301 tttttccgcg ccaaacaata cgatcaagtt ttcctctgtg ccgcataagg gcctcacggt
     3361 gcatcgtgga atgacccatg acggccttgg aaaatatttg agccgtgtca tcaccaaggc
     3421 gccgggatcc acggatgata tcgtgggcat cctccgttcg acccgcacag acgtcgtcgt
     3481 ttcatatctg ccggtcggtt cggaaatggc gacgaaatgg tatgtggaac aggtgcttga
     3541 agcgggttgc gcgttcatca actgcattcc ggtgttcatc gcctcgcaac catactggca
     3601 aaatcgtttt gcggaacgcg ggctgcctat catcggcgat gacatcaagt cgcaggtcgg
     3661 agcaaccatt actcatcgag tgctgacaaa tctctttcgc gagcggggcg tgcggctgga
     3721 ccacacgtat caattgaact tcggcggaaa caccgatttc atgaatatgc tcgaacgcga
     3781 gcgattggaa tcaaaaaaga tttccaagac caacgcagtc accagccaat tggccgtgcc
     3841 attgtccgag gaaaacgcgc atgtcggccc gagcgattac gtgccatggc tgaccgatcg
     3901 gaaattttgc tacatccgca tggaaggaac gacttttgga aatgtgccgc tgagttgcga
     3961 actgaagctg gaagtttggg actcgcccaa ttccgccggc gtggtgatcg atgcagtccg
     4021 ttgcgcgaag cttgcattgg accgcggcat cggcggtgcg ctgatcggtc ccgccagcta
     4081 tttcatgaaa tcaccaccgc agcaattcac agacagcgaa gccagggaac gcacggaagc
     4141 ttttatatca gaaaacagga gaaacgatga tcgaagccat caagttgtgg aacgagccga
     4201 acaatctgtc gcattgggat tttcagatgg acccgggctg gaaacagttc gtggaaatga
     4261 cgcgcatggc cgccatcacc atacggcgac tctgcccgaa actgccgttg attctcggag
     4321 ggatttcccc cattgatccc gatttcatcg cccttctcga tgcccaagga ctgctggacc
     4381 atcttgacgg cgtggccgtt cacggtttcc ctctggattg gaatcactgg caaattgacg
     4441 agtggccgaa caagctggat gaaattcgag ctgtcacttc gttgcccatt tgggtgaccg
     4501 aagtgggagt ttcgaccttt ggcgcggatg aagtgcaagt cttcggtttg caacgcacaa
     4561 cggaattgtt gcatggacga gcggagcgga ttttttggta cagcctgctc gatctcccgc
     4621 gcaaatggga ggccaccacc cgccacaaag agagtgaagg cagtgcatat taccggcact
     4681 tttacatggg attgattcgc gaggacggca cacccaaacc ggcggttaag catttcaatc
     4741 cggaacttgg aatctgccaa tggtttcatt tccaggatca ccggttggac ttcgccgtgg
     4801 gttggttgcg aaaactcggg gtcaaaaaat tgcgcacagg catcagttgg gctgactggt
     4861 gccgaccggg cgcaatcgaa tggtacgacc ggcagatgga tgcgctgaaa gatttcgaca
     4921 ctaccatcac gctttgcttc acgccgcctt cgcggggcaa ggcgcccaat tgcaccagcc
     4981 cgcccgttga acctgcggag ttcgcctggt tcgcgcgcga ggtggtgcaa cgttatgtgc
     5041 tctcgcccga aacaggagcc acgaatgact acgttctatc tgattcgcca tgcgtcgaat
     5101 gacgtgcttg gcaaggcgat tacaggcaga aagccgggtg tgagcctgaa tcctcaaggg
     5161 ctgtgtgaag ctgaaaattt ggcggcgcga ctggcgggaa aaccgattca aatgatcttc
     5221 agcagcccct tggaacgcgt tgttgaaacc gccgtgccgc tggcaaagcg gctggggttg
     5281 gacatccaga tttccgaagc gctcaatgaa attgattttg gcaactggac gcatcaaacc
     5341 ttcgaacact tggcggaact gcccggctgg caacgatgga attctttccg cagcggcacg
     5401 cgcgcgccaa acggggaaac catgctggaa gcccagacgc gaatcgttac ggaaatccag
     5461 cggctgcgcg caaaattccc agagcaaaca ctggcatttt tcagtcacgg ggatcttctc
     5521 cgcgcggcgc tcgcatattt tcttggcatc ccgttggatt tgtttcaacg catggaagtg
     5581 tccccggcat cggtcagcat cttgcaactt aatgaacatg agccaaaggt ggtttgtgtg
     5641 aatgcacaag gttcgctgtg agcttcagca gttcgcgcaa ttgctcgcga taattttcaa
     5701 atgcctgtcg tgtgcgcaca aattcttccg catctgcggc acaagcatcg gcacaaaccg
     5761 aggcttgccc catgcgaaac gccgcataag acatttgata aaaatcgagg acgtctgttc
     5821 gcagagaaat gcctgctcct tgcaatgctg acagcagcag tttggaagaa gagaagcccc
     5881 attcaaccat cgttcccgct gtatcccaat acaccggctg acgaccaatc atcgtatgat
     5941 ccatgtcatg gcccgatggg tcgagcttca ggatttgtcc gtcgccagtg cgcctccatt
     6001 catgcggggc aagccggccg tcgccataag ctggacagat gcttggtttg cgaactttgt
     6061 ttgcaaaccg taacgcgtgt tcggccattt cgccgcccag agcttccctt gtattccaat
     6121 acaacatttc acaaagacat tcgaaggacg tcgcttgttg cgcatcagaa agtggtgctt
     6181 tcgaaacagc ggaaatgtat cgcccgaggt gacgaattat ttccggattc tgagcgtcct
     6241 caacagaaag cggccttcct tcaacccacg gcagcgccac aaagccacgg aaccacccca
     6301 ataactttgg cgtccacacg tgatcgctat gctctttgag tgaagccgac gatttgtcag
     6361 caacagattt tcgcaaccca acgaatttcc aaagaatcga agcatcgctt ttggccgtgc
     6421 aaagaaattt cattcgttca aagctgatcg cagcccatgg ccggttggct ggctttggat
     6481 aaattttttc gcgccatcgg ccgccgctca aatcttcaac ggtggtgaac tgttcttgag
     6541 ctgcaatctc gcgagattct tcccgcaagc attccggaag gcccattcca ctccaacgca
     6601 actgctccca aggggtcaca tatcgacgga cacgtttcca ccatccgcgc acttcatctg
     6661 aagctgccgt gcccggttct ccaccgtgtc ccggcaagaa tgaaatctgt tccggttcaa
     6721 agccaatttc aacgagtgct tttgcaacgg ctgccattga agaaccagag atccccggcc
     6781 cctcatccac cacgagcgca cggcgagcgc atccatgaac ggaatcaggc agttccacca
     6841 ccctggcaaa cggaggaccg ctcggacgca cggtaattct cataggccgc caaccagccg
     6901 atgcaagcgt cgcggaaact accgccgaca gcgcggttcc tatgctgcga atgccaacga
     6961 tcaaaacgcg gtcgccaaga tgattcaaat tacagttgct ccaggacaac gccgaataac
     7021 agtattgttc aggataaagc gcataaaatt caaaaccttc cggaattttg accgtcacgg
     7081 gttcatctgg aaaacgggag gcatcgctca acgttttgcg taagcaatcc aaatccccgc
     7141 tgatattcac cgacggaatt cgccagtccg tctggcatcg cgtccaagcg gcataaaatg
     7201 cagcggcggc gcaatcgctg atttttcgcg cgcacgcggc atgggcccga tacgaagggc
     7261 tgtcttcaat cgcctgctcc aactcccctg cttgaatcaa cagcgagcga agttcgtcca
     7321 catcatcgcc acagaactca agagcttttt gtcgcaactt atccagcagt tcggcgggac
     7381 tcgcttcaaa gtgtggatcg ccataaacaa tcacccgcaa aattaacttt gaggcaatcg
     7441 aacggcaaat ggggcaaaac tgttcttttg tcccgacgag caatttaccc cattgagcgg
     7501 cgagcgccgt gcagtcaaag tgagttggcg ggacgagcat ggacctatgg ctcatcgtac
     7561 acaaaggcgt aacgaccatt ctaattgacg caatctcatg cgcggcaatc tgcttgttta
     7621 ttggttttcc tgggtgaaca cgatttgctg ggtcgtatgc ttttggtgga tgcaccgcat
     7681 ttccgcgcgg caggacgcgt tcatgaaggc actcaaggac caggggattc gcatcgaaag
     7741 gctttccaaa gttgagcacg acatgctcaa ggaggtgcat ccacaggttg gcgaaataaa
     7801 acaagcggtg accgaagtgg tgcaatccac ccgcgaacaa caggaaaccc tgccgcagac
     7861 aaaaccggca agataaaaaa cgcgttttgc cggaaggtga acaacaactt tttacatggc
     7921 aaagaaaaca aaagatgcag cacgtcgaag ccgcagagaa atcgcaaccg gatgtatcaa
     7981 tttgcttttc ggacggcgcc ttgccaccgg cgaagacaga caggaggagg tcggaccgct
     8041 tgccgccatc ccggttcttg gcctggacgc gctggcgtcg gccgcatacg gcccggaggc
     8101 tgcgctcact gtgctcttgc cgctcggatt gctggctcca ttgaaaatcg gaccgatcac
     8161 gattgtcatc atcgttgtgt tggcgctggt cgcgttttcg tatcaacaga ccatcagcgc
     8221 ttatccgaat ggcggtggtt cctatactgt ggcaaaggaa aatctgggca atcgctgggg
     8281 tttgttggca ggcgcggcat tgacggtgga ttacattttg aacgtcgcag tcggcatctc
     8341 cgccggcgtt gcggccatcg gttcggcttt tccgccattg ctgcgatata cacttccact
     8401 ttgccttgga ttgctcgcca ttctcacgct gatcaatttg cgaggtgtgc gcgaagccgg
     8461 cgttgcattc atgcttccaa cttacatgtt tgtcggctgc cttttcgtgg cgctgggaat
     8521 gggaactctc catgtcattt tgcatcacgg tcacccgatt cttgtaacac ccccatcgcc
     8581 tttgccgcca ccacgagaag caatcacgct ttggcttttg gtgcgggcgt tcgccaatgg
     8641 ctgcacggcg atgacgggcg tggaggcagt ttcgaacgca gtcccaatct tccgggagcc
     8701 aaaagtgaaa ttggcgaagc tcaccttgct caccatcatc ggcatcctga ttgtcctgct
     8761 cttgggcatt gcttttgtct gccgggctta cggcatcggc gcaacccagc ccggctcgcg
     8821 cgaatatcag agcgtgttgt cggaattggt cggtgctgtc gccggacgcg gttggtttta
     8881 ttatgtgagc atggcgtcgg ttttttccgt gctggcgctt tcggccaaca ccagctttgc
     8941 cggatttccg cgcctgtgcc gtctcatggc cctggaccag tatctgcctg aagcgttcgc
     9001 cacgcgtgga cgccgcttgg tttacactta tggcatcgca ttgctggcaa tcttcgcttt
     9061 tattcttctc atcatctttc gcggcatcac caacggcttg attccattgt tcgccatcgg
     9121 cgcactgctg tcgttcactt tgtcgcagtc gggcatggtt gcgcattgga ggcgacgttt
     9181 gaaagatccc catgccaggc tggccttgtg catcaatggc gcgggcgcac tggcaacggg
     9241 aatcaccgtc ttgatcgtga tggtctccaa gttcgttgaa ggagcgtgga tcacaatcct
     9301 gctgatgcca atcatcatgg tggccttgca acggttgcaa aggcatcaac ggcgcttggc
     9361 tgcggaggtt gaatcgcatg cgccactgga tttttgccgg gtggacgcgc cgatcatggt
     9421 ggttccactg accggttgga cgcgcgttgc ggaaaaagcc ttggtgttcg caacgcgaat
     9481 ttcccccgag gtgcatgcag tccaagtgtt gacgaaagaa gttccgaagg aagatttgtc
     9541 ggaacattgg gatgagcttg tggaaaaacc ggcacagcac gcgggagtca acccgcccat
     9601 cctgaaggtc gtccgttcca aataccgcca ggaactcagg cccatcgtca attttgtgcg
     9661 cgaactcgtc cggaaaaatc ctgaccgcac cgttggcgtt gtggtgcccg agctggttga
     9721 acgccggtgg taccattatt tcttcagcta tgccactttg ttgcgaggaa tgctgctgct
     9781 gcgaggcaat ccgcgaatcg tgatcatgac catgccctgg tatccgcatg gcagtgagca
     9841 cggagcggcg taaaaaaact tcccacaaat cacggttcgc agccataaac tttgagttcc
     9901 ccgccgtgca acgggatgtt cagcaccttt ctgtttttcc ccgccttgcg caaacgggcg
     9961 attgactccc gaaaaccaag ctcattgtaa acgctcattt gctcacagcg atcggcaaga
    10021 accaccgtca atttctcacg ccgcaaatct tcgctcgtcg caaaagttgg agacaatgtt
    10081 accgtcctta gccaggcagt gacgaattct tctccgtcac ttttttcaaa cacgtgcgct
    10141 tcgctgtcgg attggatgtg tctgacctcc ggcgtcgtgg aatccaggca gcaaaagccg
    10201 cctgcaaaaa gctggtgaaa aaaatccagt gcccgcagag ccggtttggg ccgtcctgat
    10261 tcatccaaca cgcccagccg aaggttgttc tcgtcgccaa tcacttgcgt gtttgcggga
    10321 agatcgtgga tgcgatacca cgcgactaat tggactttgc cggtcgccag cgttgaagcg
    10381 acggcgcgcc ccaatgtttc cgcttgaaat tccggcgtgt gctcataagc aaagcgcgcc
    10441 cggtaag
//
LOCUS       DASZMM010000143         4534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_866354, whole genome shotgun sequence.
ACCESSION   DASZMM010000143 DASZMM010000000
VERSION     DASZMM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866354"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..382
                     /locus_tag="VG754_03615"
     CDS             26..382
                     /locus_tag="VG754_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWF18325.1"
                     /translation="MATKLFVGNLPFSATENDLQDHFAGAGTVIAVNIMQDRATGRSR
                     GFAFVEMGSQAEADAAISQFHGKEFQGRPLTVNEARPREERPPGGGGGGGGYRGGGGG
                     GDRRGGGGGGGYRDRR"
     gene            complement(432..1814)
                     /locus_tag="VG754_03620"
     CDS             complement(432..1814)
                     /locus_tag="VG754_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWF18326.1"
                     /translation="MKPPNPTLLAKLQQLLPRGEISLDKSVREQHAGDKWFASHLPDA
                     VAFPRRTKSVSALLRFANEHRIPVTPRGAGFGYVGGCVPEHGGIALSLMRMDRIKEIH
                     AHDFVAVVEPGVITQTLQEAVEKRGLFYPPDPASRADCSIGGNIATNAGGPRCLKYGV
                     TRDYVLGLEVVLADGSVARLGSRTHKNKTGFDFTRLFVGSEGMLGVVTEATLKLLPLP
                     PYRACLAIGFGAMKDAARTIHAILGAGFLPCALEVADAFTLAAAGERTKSKRLSGCRA
                     HLIVELDGQEFSVRNEIKSLQRIVKRQHPLFVETGFGNRECETIWQLRREFSYALRDT
                     GLTKLNQDIVIPRGRLEDLFQFATRLQKKHGLRLACFGHAGDGNIHVNVMVDMTQPQA
                     MEHSQSALDELFRQVLAWQGVITGEHGIGLAKKPWWPLAVSPEVRELHQRVKDALDPH
                     GILNPGKFLK"
     gene            complement(1811..2038)
                     /locus_tag="VG754_03625"
     CDS             complement(1811..2038)
                     /locus_tag="VG754_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HWF18327.1"
                     /translation="MKLVTIFTAFNPTEAEVIRSRLDVSGFHSVITHGLSALSTDGYS
                     LATGGILVQVPEDEAEDARELLAAPDSDPPP"
     gene            complement(2069..3091)
                     /locus_tag="VG754_03630"
     CDS             complement(2069..3091)
                     /locus_tag="VG754_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWF18328.1"
                     /translation="MSLWYIRIFFWVLCSLAGMAISQVRPETIGHGGAIIGLLFGFGF
                     GGILIAIDEMLKGFSLRAFSATTFGLILGCVIAFLVDHSGMFEQTDSKTRWLIRLALF
                     LGCGYIGMVLAMRSNKEDFSLIIPYVRFSQQHKPDNLLLLDTSVIIDGRIADLIESRL
                     LEGLIVVPRFVLKELQQIGDSNDPIRRARGRRGLDMLNRLQRSTAAEVKIHDGDFPEE
                     KEVDAKLVRLARNLNAKLFTNDYNLGKIAELQSVAYVNIHEVAKGFKTVVIPGEMFHL
                     RLVREGKDKDQAVGYLPDGTMVVVNHGHSMIGQDVDVQVQSLLQTGAGIIVFAEIKSP
                     VAPKAA"
     gene            complement(3111..4070)
                     /locus_tag="VG754_03635"
     CDS             complement(3111..4070)
                     /locus_tag="VG754_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWF18329.1"
                     /translation="MSKSRTELKVGFFVLICLTLVAVLLLQFSKGVTLFRKTYTIILN
                     TSNVGGLKPRAQVLMSGVMVGTVSKTVLSPQGTNVAVYLKMYSQYVVPTNSTFKIEQS
                     GFLGDQYVAIYPVANQGEVLTDGAEVHANEPFNIQEVAAKVNQVIDAVQHVVLNTNTL
                     TNVAVALVHLKSASEKAEIAVDHLNALILSNSAPLTAAFSNLVTFSDQLDAVGTQAGG
                     ILETNRPKINLAMSNLYTASVMMTNLLSEVDHGNGLVAELLKDEELGGKVKEIAGGLS
                     ATSTNLAIATANLNRLGLWHFLWYHPKHQETNEPSRLPPVQQR"
     gene            complement(4067..>4534)
                     /locus_tag="VG754_03640"
     CDS             complement(4067..>4534)
                     /locus_tag="VG754_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF18330.1"
                     /translation="VAENVGFALERECKLGAAEIAKKVAGALDVVELSGTEEKKPSEL
                     SGGMRKRVGLARAIVYEPEIVLYDEPTTGLDPVVSDSIDKLIMRVRDRLKVTTVVVTH
                     DMRSARRVGQRIMMLHDKKIYVTGTPDEIFSSQDPVVHRFVNGISTPKDTLTL"
BASE COUNT         1004 a         1298 c         1249 g          983 t
ORIGIN      
        1 gataaacaac gaaagtatca agataatggc gaccaaatta ttcgtcggga accttccctt
       61 cagtgccacc gagaatgatc tgcaggatca tttcgcaggc gcaggcacag tcatcgcagt
      121 aaacatcatg caggatcgcg ccaccggacg ttcgcgcggt tttgccttcg tcgaaatggg
      181 ctctcaagcc gaagccgatg ccgcaatttc ccagttccat gggaaagaat tccaaggccg
      241 gcctctgacc gtgaacgagg cgcgtcctcg tgaagaacgt ccgccgggtg gcggcggcgg
      301 cggtggtggt tatcgtggcg gcggcggcgg cggagatcgt cgtggcggcg gcggtggcgg
      361 cggctatcgc gaccggcgtt aagcttcagc caaattttca caaaccaccg ttcgcaaagg
      421 acggtggttt tttatttgag aaattttccg gggttcaaaa tgccgtgcgg atccagcgca
      481 tccttgacgc gttgatgcaa ctcgcgcact tccggcgaaa ccgccagcgg ccaccaaggc
      541 tttttcgcga gaccgatgcc gtgttcgccc gtgatgacgc cttgccacgc aagcacctgc
      601 cggaagagtt catccagcgc ggattgcgaa tgctccatcg cttgtggttg cgtcatgtcc
      661 accatcacat tcacatggat gttgccgtcg ccggcgtgac cgaaacaagc gagtcgcagt
      721 ccatgttttt tctgaagcct tgtggcgaat tgaaacaaat cctccaaccg cccgcgcggg
      781 atgacgatgt cctggttcaa cttggtcaag ccggtgtcgc gcagggcgta agaaaattcc
      841 cggcgcaact gccagatggt ttcgcattcg cgattgccaa aaccggtctc gacgaacaag
      901 ggatgttgac gttttacaat ccgctgcaac gatttgattt cattgcgcac agagaattcc
      961 tgtccatcca gctcgacgat aagatgcgcg cggcaaccac tgagccgttt gcttttcgtg
     1021 cgttcgccgg ctgcggcaag cgtgaatgca tccgccactt ccaaagcgca gggcaaaaaa
     1081 cccgcgccaa gaatcgcgtg gattgtccgc gcggcgtcct tcatcgcgcc aaagccgatt
     1141 gcgaggcagg cgcgatacgg cggcaatggc aacaacttca aagtcgcttc ggtgacgacg
     1201 ccgagcattc cttccgagcc gacgaacagg cgcgtgaaat caaatccggt tttgttcttg
     1261 tgcgttcgac tgccaagccg tgcgacggag ccatcggcca gcacgacttc aagtccgagg
     1321 acgtaatcac gcgtcacgcc gtatttcaaa caacgcggtc cgccggcatt cgtggcgatg
     1381 ttgccgccaa tggaacaatc ggcccgactc gccgggtcgg gcggataaaa cagcccgcgc
     1441 ttttcgactg cttcctgcaa agtttgcgtg atgacaccgg gttcgacaac agcaacgaaa
     1501 tcgtgcgcgt gaatttcctt gatgcgatcc atccgcatca gcgaaagcgc gatgccgccg
     1561 tgttcgggaa cgcatccgcc gacataccca aatcccgcgc cgcgcggcgt gacaggaatc
     1621 cgatgctcat tggcgaaacg cagcaacgcc gaaaccgatt tcgtcctgcg cgggaacgcg
     1681 acagcgtccg gcaaatgaga cgcgaaccat ttgtccccgg cgtgctgttc gcggaccgat
     1741 ttgtcgaggc tgatttcgcc gcgcggcaga agttgttgga gtttggcgag taatgttgga
     1801 ttgggcggct tcatggcggt gggtctgagt caggcgcggc caacaattca cgcgcgtcct
     1861 ctgcttcatc ctccggcacc tgcacgagaa tgccgccggt cgcgagcgag taaccatccg
     1921 tgctcagcgc ggatagtcca tgggtgatga ccgaatgaaa acctgagaca tccagccgcg
     1981 accggatcac ttctgcttcc gtcgggttga acgcggtgaa aatggtgacc aatttcatgg
     2041 ttaacattgc ttccaaagga agttttgttc aagccgcctt cggggcaacg ggagatttga
     2101 tttccgcgaa tacgatgatt cccgcgcctg tttgcagcag gctttgcacc tgcacgtcca
     2161 cgtcctggcc gatcatgctg tggccgtggt tcacgacaac catggtgccg tccggcagat
     2221 aaccaaccgc ctggtccttg tccttgcctt cgcgcacgag ccgaagatga aacatctcgc
     2281 cgggaatgac gacggtctta aaacctttcg cgacctcgtg aatattcacg taggcgacgg
     2341 attgaagctc ggcgattttt ccaagattgt aatcgttcgt gaaaagcttc gcgttgagat
     2401 tccgcgcaag ccggacaagc ttggcgtcca cttccttttc ctcgggaaaa tccccgtcat
     2461 ggatttttac ttcggcggcg gtgctgcgtt gaaggcggtt gagcatgtcg agtccgcggc
     2521 gtccgcgcgc gcgccggatt gggtcattcg aatccccgat ttgctgcaat tccttcagca
     2581 caaaacgagg caccacaatg agaccttcga gcagcctgga ctcgataagg tccgcgatgc
     2641 gtccgtcaat gatgacgctc gtgtcgagca gcagcagatt gtcgggcttg tgttgttgcg
     2701 aaaagcggac gtagggaatg atgagcgaaa aatcttcctt gttgctgcgc atcgcgagca
     2761 ccatgccgat gtaaccgcag ccgagaaaaa gcgcgaggcg gatgagccag cgcgtctttg
     2821 aatccgtttg ttcgaacatg cccgaatggt ccaccaaaaa tgcgatcaca cagccaagga
     2881 tcagaccaaa cgttgtcgcc gaaaatgcgc gcaacgaaaa tccttttagc atttcgtcaa
     2941 tggcgatcaa gattccgccg aaaccaaacc cgaacaacag gccgatgatg gcgccgccat
     3001 ggccgatggt ttcaggccgg acctggctga tggccatgcc ggccagcgag cagaggaccc
     3061 aaaagaaaat gcgtatatac cagagtgaca tggaggttaa ttattgtgag tcatcgctgt
     3121 tgaaccggcg gcaatcgaga tggctcgttc gtttcctgat gcttcggatg ataccagaga
     3181 aaatgccaaa gacccagccg gttcagattg gccgtggcga tggcgagatt ggtggaagtt
     3241 gcggacaacc cgcccgcgat ttccttcact ttgccgccaa gctcttcatc tttcaacaac
     3301 tcggcgacca acccgttgcc atgatccact tcgctcaaca gattcgtcat catcacggac
     3361 gcggtgtaaa gattgctcat ggccaggttg attttgggac ggtttgtttc aaggatgccg
     3421 ccggcctgcg tcccaactgc gtccaactga tcggaaaatg tcaccaggtt gctgaacgcg
     3481 gcggtaagcg gcgcgctgtt ggacagaatc aaggcattga ggtgatcaac ggcaatctcc
     3541 gccttttccg aggcgctttt taaatggacc aacgccaccg caacatttgt caacgtgttg
     3601 gtgttgagca ccacatgctg gacggcatca atgacttgat tcacctttgc ggccacttcc
     3661 tggatgttga aaggctcatt cgcatggacc tccgcgccgt ctgtgaggac ttcaccctga
     3721 tttgcaacgg gataaatcgc cacatattga tcgcccaaaa aacccgactg ctcgatcttg
     3781 aatgtggaat ttgtcgggac aacatattgg ctgtacatct tgaggtaaac tgcgacgttt
     3841 gtcccttgtg gtgaaaggac cgtcttcgag acagtcccca ccatcacgcc ggacatcagc
     3901 acctgcgcgc ggggtttcaa gccgccgaca ttggacgtgt tcaggatgat ggtgtaggtc
     3961 tttcgaaaaa gggtgacgcc cttgctgaat tgcagcaaca gcaccgcaac caatgtcagg
     4021 catatcagga caaaaaagcc gactttcaac tcagtgcgag atttgctcat aatgtcaaag
     4081 tgtctttggg tgtggaaatg ccattcacaa aacgatgcac gaccgggtcc tgcgaactga
     4141 aaatttcgtc cggtgtgccg gtcacataga tttttttgtc atgcaacatc atgatgcgtt
     4201 gcccaacccg ccgggcgctt cgcatgtcgt gcgtcaccac caccgtcgtc accttcaagc
     4261 ggtcgcgcac acgcatgatc agcttgtcaa tgctgtcgga aaccaccggg tccaagccgg
     4321 tggtcggttc gtcataaagc acaatctccg gttcataaac aatcgcccgc gccaggccaa
     4381 cacgcttgcg catcccgccg gacaattccg aaggcttttt ttcttccgtc ccggaaagct
     4441 ccaccacatc cagcgcgccc gcgactttct tcgcgatctc cgctgctccc agcttgcatt
     4501 cccgttcaag cgcgaacccg acattctccg ccac
//
LOCUS       DASZMM010000144         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3616639, whole genome shotgun sequence.
ACCESSION   DASZMM010000144 DASZMM010000000
VERSION     DASZMM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3616639"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VG754_03645"
     CDS             complement(<1..1295)
                     /locus_tag="VG754_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18331.1"
                     /translation="MNGDLVARIHFAGTTRLTSDPAAVKLNQLAALPETAQLREMVLQ
                     KLSTAPFRLFQNKLANGNTNDFSWLLRPLVDDLMREESYVEMRGPSNAIPELMLAVHL
                     NSTRAKAWHDSLSKVLSTWTGIPVKEIQGDGFSGWELRKHHDPNLIRCLRAGDWVLFG
                     WGQDELRLQPAFLQRIKTQKRPVEDFKEGFLDASVDLQTLMRYHPFSLLALFPSPLPK
                     VHLTEMMRKDYVRSKLVMQFPEPLNLKLDPWKIPTNTIHNPIVNFTAVRGVASWLGRL
                     EVVKKTQPPSVPNQLCFWSMPKLTFDIGMTAPVSDAGKYMAQIGPRLAPALDSYFNEH
                     ALLGKAEWTNHQVRLSKLPFIAPYLAPTRDAKSDFLLGSLMPVRHTNSLVFPPELMRE
                     LLSKPNLVYYGWEINEESVVQWQALQQIYLMDAVKPLPS"
     gene            1483..1941
                     /locus_tag="VG754_03650"
     CDS             1483..1941
                     /locus_tag="VG754_03650"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HWF18332.1"
                     /translation="MPKVRSFLLYWVPVIVWMSLIFTASGDTGSFQHSSRIIGPLLRW
                     LFPSISHETEDAIVFGVRKCAHLTEYAVVAFLVWRGIRKPRWKDPRPWKWSEAGVALW
                     VAMFYATTDEFHQTFVPSREGCVRDVLIDSTGAVVGMFALWLLGRLFKKW"
     gene            1945..>4203
                     /locus_tag="VG754_03655"
     CDS             1945..>4203
                     /locus_tag="VG754_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWF18333.1"
                     /translation="MKKRPLIAVALFYVAGILLAQLPILLLPLFLIAFTLIVLFAFWT
                     AARPALLCALIILAGWLNLAERTAVLSPYDLRKIVASREELASVRGKLIETPYHRVHE
                     IKDNEIWSSMARMEVSAIRIGESGWQPAHGSIVTSTKEFLPDQFFAGQIVEATGVLRK
                     ARGPVAEGLFDYGKFLEHQGIYHELDVKLVSDWKIISTPPRPPLADRFCAWARKTLAL
                     GLPEEDESVRLEWALSLGWKAALTDKVSEPFIKAATYHIFAVDGLRIAIISGIFLALF
                     RVMHVPRAWCGLLTVPFLFFYAAMTGWPASAIRAIVMISVIFGGWALKRPSDLINSLF
                     MAALVILVWEPRQLFQAGFQLSFFVVLCIILILPFFNSVGKLLLRTDPLLPEQLLTGW
                     QKMWRIPARWIVDLFLASVAAWLGSIPLVVWYFHLVTPISGPANVFAVPLCALVLICN
                     LASLFFAPWLTYLSVLFNNAGWFLMECIRATSGWSADWPGAYFYLPMPGFFTIALYYA
                     LLLTMLTGWLFTKKFRVRKIGSAILLIIIWCGLWLWERPAARLTVLPLNGGHAVYLQM
                     PKGREDWLIDCGNESAATTIVKPFLQAHGVNRLSNFLLTHGEISYSGGAPLITEQFRP
                     RNLYTSPVHFRSPSYREFESTASEQPDWRNPLQAGDQIGLFTVLYPAPACKTASPKAS
                     DNALILRGEIYGTRFLLLSDLGHLGQNALLNSAETNSTDRNFLQADIVIAGLPGFEDD
                     EPLSDALLHAIQP"
BASE COUNT          877 a         1231 c         1080 g         1015 t
ORIGIN      
        1 tttgaaggca aaggcttgac cgcatccatc aagtaaatct gttgaagtgc ttgccattgc
       61 acaacgcttt cctcgttgat ttcccaaccg tagtaaacaa ggtttggctt ggacagaagt
      121 tcgcgcatca attccggtgg aaacacaagg gaattcgtgt gtcgaacagg catcaagctt
      181 cccaggagaa aatcgctttt cgcatcccga gtcggggcta aatagggggc aataaaaggc
      241 aacttcgaca acctcacttg atgattcgtc cattccgcct tgcccagcaa ggcgtgctcg
      301 ttgaaatatg aatcgagtgc gggtgccagg cgcggcccga tttgcgccat atacttccca
      361 gcatcggaaa caggcgcggt catgccgata tcgaacgtca gcttgggcat cgaccaaaag
      421 caaagctggt tcggaacaga aggcggttgc gtttttttca caacttccag ccggcccaac
      481 cacgatgcaa cgccccggac agccgtgaaa ttcacaatcg gattatggat ggtgttcgtc
      541 gggattttcc acggatccag ctttaaattc aacggctcgg gaaactgcat cacgagcttg
      601 gatcgcacgt aatcttttcg catcatctcg gtgagatgca cttttggcaa tggggacggg
      661 aacagcgcga gcagactgaa tggatgatac cgcatcaacg tctgcaaatc cacggacgcg
      721 tccagaaagc cttccttgaa gtcctcgacc ggacgttttt gggtcttgat gcgttgcaag
      781 aatgccggtt gcaaccgcaa ctcatcctgg ccccaaccga acaacaccca atcacccgcg
      841 cgcaagcatc ggatcagatt tggatcatga tgtttccgca attcccaacc ggaaaaacca
      901 tcgccttgga tttccttcac aggaatgcct gtccacgtgg acagaacttt tgacaaactg
      961 tcgtgccacg ctttggcgcg ggtgctgttc agatgcaccg ccagcatcaa ctccgggatc
     1021 gcgttgctcg gcccgcgcat ctccacgtac gactcttcgc gcatcagatc gtccaccaat
     1081 ggccgcagaa gccaagaaaa atcgttcgta ttgccgttgg cgagcttgtt ttgaaacagg
     1141 cgaaatgggg ccgtcgaaag cttttgtaaa accatttccc gcaactgcgc cgtctcgggc
     1201 aatgcagcca gttggttcaa tttcacggcg gcaggatcgg acgtgaggcg cgtcgtgccc
     1261 gcaaaatgga tacgcgccac cagatcgccg ttcatgctcg ataacgcttc atcggtcgcc
     1321 gcactggcaa ctcgatggcc aatgctcaag acgacacata cacccattaa aatacaacga
     1381 ttacgcatag gaaccggttt ttgtgccaga aacgagcgct tttggcaaat cgaatccatt
     1441 aaaaaagttg cctctcgccg ccgaaccttg ctttcatgct gcgtgcctaa agtccgttca
     1501 tttctgcttt attgggtgcc ggtgatcgta tggatgtcgc tcatctttac cgcatcggga
     1561 gataccggtt cgtttcaaca ttcctcgcgc atcatcggcc ccctgctccg ctggcttttt
     1621 ccaagcatct cgcatgaaac cgaagacgcc atcgttttcg gcgtacgcaa atgcgcgcac
     1681 ctgaccgaat acgccgttgt cgcgtttctt gtctggcgcg gcattcgcaa accgcgttgg
     1741 aaagatcccc gcccgtggaa atggtccgaa gcgggcgtgg ctttgtgggt ggcgatgttt
     1801 tatgcgacga cggatgaatt tcaccagacc ttcgtgcctt cgcgcgaagg ttgcgtgcgc
     1861 gatgttctga ttgattcgac cggcgcggtg gtcggaatgt tcgcgctctg gctgctcggc
     1921 aggcttttca agaagtggta accaatgaaa aaacgcccgc tcatcgctgt ggcgttgttt
     1981 tatgttgcgg gaattctact ggcgcaacta cccattctgt tactgccgct cttcctcatc
     2041 gcgttcacgc tgatcgtttt attcgccttc tggacagcgg cgcgtcctgc gctcctttgc
     2101 gcgctgatca ttctcgcagg ctggctcaat ttggccgaac gcaccgccgt tctttcgccg
     2161 tatgatttgc gaaaaatcgt cgccagccgc gaagaactgg catctgtgcg tggcaaactg
     2221 atagagactc cctaccatcg cgttcacgaa atcaaggaca acgaaatctg gagttccatg
     2281 gcgcgaatgg aagtgagcgc aatccgcatc ggcgagtctg gatggcaacc cgcgcatgga
     2341 agtatcgtca ccagcacgaa agaatttctg ccggatcaat tttttgccgg ccaaatcgtc
     2401 gaggcgaccg gcgtgttgcg caaggcgcgc ggcccggtcg cggaaggatt gtttgattat
     2461 ggaaaatttt tggaacatca gggcatttat catgaactgg atgtcaaact cgtctcggac
     2521 tggaagatca tttcaacgcc gccgcgcccg ccgcttgccg accgattttg cgcatgggcc
     2581 agaaaaacgc tcgcgctcgg gctgcctgaa gaagacgaat cggtgcggct ggaatgggcg
     2641 ctttcgcttg gctggaaagc cgcattgacc gataaagtct ccgagccatt tatcaaggct
     2701 gcgacctacc atatttttgc agtcgatggg ctgcgcatcg cgatcatctc cggaattttc
     2761 ctggcgctgt tccgcgtcat gcacgttccg cgcgcctggt gcggcctgct gacggtcccg
     2821 ttcctgtttt tttacgcggc gatgaccggc tggcccgcat cggccatccg cgccatcgtg
     2881 atgatcagcg tgattttcgg cggatgggcg ttgaaacggc caagcgacct catcaattcc
     2941 cttttcatgg ctgcccttgt cattctcgtt tgggagccgc gccaactgtt tcaagcggga
     3001 ttccagctct cattcttcgt tgtgctctgc atcatcctga ttcttccctt cttcaatagt
     3061 gtcggcaaat tattgctgcg caccgacccg ttgctgcctg aacaattgct gaccggctgg
     3121 caaaaaatgt ggcgaatacc ggcgcgttgg atcgttgatt tgtttctcgc ttccgtcgct
     3181 gcgtggctcg gttcaattcc gctggttgta tggtatttcc atctcgtcac acccatcagc
     3241 ggcccggcga acgtcttcgc tgtgccgctg tgcgcgctgg tattgatttg taatttggcc
     3301 agcttgttct ttgcgccatg gctgacgtat ctcagcgtgc tgttcaacaa cgccggctgg
     3361 ttcctaatgg aatgcatccg cgcgaccagc ggttggtccg cggactggcc cggcgcgtat
     3421 ttctacttgc cgatgcccgg cttcttcaca atcgcgcttt actatgccct gctgctcaca
     3481 atgctgactg gctggctgtt cacgaaaaaa ttccgcgtca ggaaaatcgg cagcgcaatt
     3541 cttctgatca tcatctggtg cggcctttgg ctttgggaac gccctgccgc gcggctgacc
     3601 gtgctgccgt tgaacggagg tcacgccgtt tatctccaaa tgccgaaggg ccgagaagat
     3661 tggctcattg attgcggcaa cgaatccgcc gcgacaacca tcgtcaaacc gttccttcaa
     3721 gcgcatggcg tgaatcgtct ctcgaatttt ctcctcaccc acggcgagat ttcctattcc
     3781 ggcggcgcgc cgctcatcac cgaacaattc cgtccgcgca atctctacac aagtcccgtc
     3841 catttccgct cgccaagcta tcgcgaattt gaatccacgg catccgaaca acctgattgg
     3901 cgaaatcctc tgcaagccgg cgaccaaatc ggtctcttca ccgttcttta tcctgcaccc
     3961 gcgtgcaaaa ccgcgtctcc caaagccagc gacaacgccc tcatcctacg tggtgaaatt
     4021 tatggcaccc ggttcttgct cctctccgac ctcggccacc ttggccaaaa cgcgctgctg
     4081 aactctgccg aaaccaacag cacagaccga aatttcctcc aagccgacat cgtcatcgcc
     4141 ggtcttcctg gtttcgaaga cgacgaacca cttagcgatg ccttgctcca tgccatccaa
     4201 ccc
//
LOCUS       DASZMM010000145         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3073129, whole genome shotgun sequence.
ACCESSION   DASZMM010000145 DASZMM010000000
VERSION     DASZMM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3073129"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VG754_03660"
     CDS             complement(<1..356)
                     /locus_tag="VG754_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWF18334.1"
                     /translation="MQIELEHREITEKIIGAAFEVYRVLGYGFLESVYQKAMQVELLR
                     VGLKCELEHPITVQYKGAIVGYFQADLFVNDAVMVELKTAKNYNPEDEPQLLNELKAT
                     GIKVGLLINFGRTKVE"
     gene            complement(382..1830)
                     /gene="sufB"
                     /locus_tag="VG754_03665"
     CDS             complement(382..1830)
                     /gene="sufB"
                     /locus_tag="VG754_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWF18335.1"
                     /translation="MSTATETIEEFVNKEYQYGFVTNVETDTIPPGLSEDVVRLISEK
                     KKEPEFMLNWRLKAYRHWLTMTEPKWQKAHYPPIDYQASIYYAAPKPKKTLNSLDEVD
                     PELLKTFDKLGISLNEQKRLTGVAVDAVVDSVSVATTFQKDLAKLGIIFCSFTEAVQK
                     HPDLVEKYLGSVVPYTDNFFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINTANTG
                     QFERTLIIAEEGAYVSYLEGCTAPMRDENQLHAAVVELIALGRKSQIKYSTVQNWYPG
                     DKNGKGGIYNFVTKRGKCLGESSKISWTQVETGSAITWKYPSVILQGDNSQGEFYSVA
                     LTNNWQQADTGTKMIHVGKNTSSTIISKGISAGHGQNSYRGLVKILKNATSARNFSQC
                     DSLLLGDKCGAHTFPYIEVKNTSSRVEHEASTSKIGEDQIFYCNQRGISTQDAVNMIV
                     NGFCKEVFRELPMEFAVEAQKLLGVSLEGSVG"
     gene            complement(1903..2367)
                     /locus_tag="VG754_03670"
     CDS             complement(1903..2367)
                     /locus_tag="VG754_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HWF18336.1"
                     /translation="MSSKHNDEVDASLNKRLATSGFRFTPQRQHVYNVLLQKRDHPTV
                     EEVFIRSKDEMPDISMATVYNCLDALVKCGLVRHVNLDRSATRYCPNMKEHCHFYCDD
                     CDGIFDVDFSADGERGGLQLPDGFKVKQFDISIRGICRDCATRRSLDPKAKP"
     gene            complement(2466..>3349)
                     /locus_tag="VG754_03675"
     CDS             complement(2466..>3349)
                     /locus_tag="VG754_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HWF18337.1"
                     /translation="EFPLAMQPLSSSAPKTARSAVVGQVARDYADDHFPLLILGDDSY
                     GDFTFKTKFKIVDGLTEQVAGIAFRIQDEKNFYYVRADAVANKLSFCGVLKGANHTLY
                     SKDRPVQKGVWHELVVQCEGPKIHIGVDGEFLDWISDLTFSAGKIAFCTKSDSIVHFG
                     DPSMTYTPREPFVQMIVRDMMKEYPRLQGVKIYMVPPKQKDVKLVASNNEAEIGQPGE
                     KTDADVIDRGVNYYRRDKEMIYITMPLRDRNGDPVAAVRFVIKGFPGQTQDNAMVRVT
                     PVLKKMQLRASAAESLF"
BASE COUNT          737 a          905 c          847 g          860 t
ORIGIN      
        1 aattcaactt tcgtccgtcc gaaattgatc agcagtccaa cttttattcc ggtggccttc
       61 aattcattca ataattgcgg ttcgtcttca gggttataat tcttcgcagt tttaagttct
      121 accatcaccg catcattgac gaacaaatcc gcttgaaaat aaccgacaat cgcgcctttg
      181 tattggacgg ttattggatg ctcaagctcg catttcaaac cgaccctcaa caattccacc
      241 tgcattgctt tctgataaac actttccaag aacccataac ccagcacacg atagacctcg
      301 aaagcggcgc caatgatttt ctcggtgatt tcgcgatgct ccagttctat ttgcatccgt
      361 gttcaatccg tggctaaaaa atcatcccac actgccttcc agactcaccc ccagcagctt
      421 ttgcgcttcg acggcgaatt ccatcggcaa ctcgcggaaa acttccttgc agaaaccgtt
      481 cacgatcatg ttgacggcgt cctgggtcga gatgccgcgc tggttgcaat agaaaatctg
      541 gtcctcgccg atcttcgaag tcgaggcttc gtgctccacg cgcgacgagg tgttttttac
      601 ctcgatgtaa gggaaggtgt gcgcgccgca tttgtccccg agcagcaacg aatcgcattg
      661 tgaaaaattc cgggcgctgg tcgcattctt caagatcttc accagcccgc gatagctgtt
      721 ctggccgtgc ccggcggaaa tgcccttgga aatgatggtg ctgctggtgt tcttgcccac
      781 atggatcatc tttgtccctg tgtcggcctg ctgccagttg ttggtcagcg cgaccgaata
      841 aaattcgccc tgcgaattgt ctccctgcaa aatcacgctg ggatatttcc aggtgatggc
      901 cgaaccggtt tcgacttgcg tccaggaaat cttcgagctt tcgcccaggc acttgccgcg
      961 cttggtgacg aaattgtaaa tgccgccctt gccgttcttg tcacccggat accaattctg
     1021 cacggtcgaa tatttgatct gtgattttct gccgagcgcg atcaattcca ccaccgccgc
     1081 gtgcaactgg ttttcgtcgc gcatcggcgc agtgcagcct tcgaggtaac tcacatacgc
     1141 gccttcctcg gcgatgatga gcgtgcgctc gaattggccg gtgttcgcgg tgttgatgcg
     1201 gaaataggtg gacaattcca tcgggcaacg gacgcctttg gggatgtaac aaaacgagcc
     1261 gtcgctgaat accgctgaat tgagtgcggc gaaaaagttg tccgtgtaag gcaccacgct
     1321 gccgagatat ttctcgacca ggtcgggatg tttctgcacc gcctcggtga aggaacagaa
     1381 aatgatgccg agcttggcga ggtctttttg aaacgtcgtc gccacggaga cgctgtccac
     1441 cacggcatca accgcgacac cggtgagacg cttttgttcg ttgagcgaga tgccgagttt
     1501 gtcgaaggtc ttcaacaatt ccgggtccac ttcgtcgagg ctgttgagcg tcttcttggg
     1561 cttcggcgcg gcgtaataga tgctggcctg gtaatcgatc ggcggataat gggccttctg
     1621 ccatttcggc tcggtcatcg tcagccaatg gcgatacgcc ttgagccgcc agttgagcat
     1681 gaattccggt tcttttttct tttcagaaat aagccggaca acgtcctcgc tgagtccggg
     1741 cggaatggtg tcggtctcga catttgtcac gaacccgtac tggtattcct tatttacaaa
     1801 ttcctcaatt gtctcggttg ctgtgctcat ggttcactaa aatcgttaaa ttgttaaaat
     1861 tgtttgactg gcaactttta cccgtcgcct ctttttaatg ggtcaaggct ttgccttggg
     1921 gtcgaggctc cgcctcgtgg cgcaatcacg gcagatgccg cggatggaaa tgtcgaattg
     1981 tttgaccttg aatccgtccg gcaattgcag accgccgcgt tcgccgtcgg ccgaaaaatc
     2041 cacatcaaaa atcccgtcgc aatcgtcgca ataaaaatgg caatgctcct tcatgttggg
     2101 gcaatagcgg gtcgcggaac gatccagatt gacgtgacgc accaagccgc atttcaccag
     2161 cgcatccaaa caattataaa ccgtcgccat cgaaatgtcc ggcatctcat ccttggaacg
     2221 gatgaaaact tcctcaaccg ttggatgatc gcgtttctgg agcagcacat tgtaaacgtg
     2281 ctgacgttgc ggcgtaaaac ggaacccgct ggtagccaag cgcttattca agctggcatc
     2341 cacttcatca ttgtgctttg aactcattgc agggtcatcc tatcgcccga ccaagggttt
     2401 gtcaatattt ggaataattc taaatatcga atgaatgtgg cgaggctgta ggaggttgta
     2461 ggagattaga acagactctc cgccgccgag gcgcgcagtt gcattttctt gagcacgggc
     2521 gtgacacgca ccatggcatt gtcctgcgtc tggccgggga aaccttttat cacgaaacgc
     2581 accgccgcaa ccgggtcgcc attgcgatcg cgcaacggca tggtgatgta gatcatctcc
     2641 ttgtccctgc gataataatt cacgccgcgg tcaatcacat ccgcatcggt tttttcaccg
     2701 ggctggccga tttcagcttc gttgttgctg gcgacgagtt tgacgtcttt ctgcttggga
     2761 ggaaccatgt aaatcttcac gccttgcaat cgcggatatt ctttcatcat gtcccgcacg
     2821 atcatctgca cgaacggctc gcgcggcgtg taggtcatgg atggatcgcc gaaatgaacg
     2881 attgaatctg acttggtgca gaacgcgatt ttgccggcgg aaaacgtcag atcggaaatc
     2941 caatccaaaa actcgccatc aacgcctata tgaatctttg gcccttcgca ttgaaccacg
     3001 agttcatgcc agacgccctt ttgaacaggc ctgtccttgc tgtaaagcgt gtgatttgct
     3061 cccttgagca cgccgcaaaa agaaagtttg ttggcgaccg catcggcccg cacgtaataa
     3121 aaattttttt cgtcctggat tcggaaagcg atgcctgcca cctgctccgt caatccatca
     3181 acaatcttaa acttcgtttt gaaggtgaag tcgccataag agtcatcgcc caaaatgagc
     3241 aacgggaaat ggtcgtccgc gtaatccctg gcgacttggc ccacgacagc gcttcgggct
     3301 gttttgggag cgctgctgct gagcggttgc atcgccagcg ggaattcgt
//
LOCUS       DASZMM010000146        11214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_325892, whole genome shotgun sequence.
ACCESSION   DASZMM010000146 DASZMM010000000
VERSION     DASZMM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325892"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VG754_03680"
     CDS             complement(<1..345)
                     /locus_tag="VG754_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWF18338.1"
                     /translation="MLRQGTHEFTVSTRRRGFYEITRAVAAWIDESGIKNGLLTLHVR
                     HTSASLLIQENADPEVRRDLERFFSRLVPDGDPLFQHTSEGEDDMPAHVRSALTVVNL
                     SIPIINGQLALGT"
     gene            complement(440..2401)
                     /locus_tag="VG754_03685"
     CDS             complement(440..2401)
                     /locus_tag="VG754_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18339.1"
                     /translation="MKRFLAVLFLSVLVMLPTARAEGPDDDYVTIYGMIQQGDALTEK
                     GDWGAAITKYVEAQGALKRFQKAYPDAYLKVVKFRLNYLANKIEQLSSQAPKPATNAQ
                     PPAATNAPPPAPVPAAPTSPAPATAPENKAPANPTAPPPSAEMENKMKALEDQVRHLE
                     ADRSLLEAKLKEALAARPAEVDPQELAKAREQITSLQKENELLKANLESAKTNSALAA
                     AAPADLEKTKQALAEANRKVSALTEANATLAQEKEALQTRMKTLNVADPAAAALREEN
                     ELLKKQVAQLKKGAPATPSEALDKKLQEAQAQIAAMQSDKEILRLEKIALENRVRQLS
                     SITPLVPAYSSTNGAPAVSIAETNSTSPEMSDSVMASKIAQLEAQRDELQKSLEAATR
                     DAAGRRKGKEMAARLEEMSRQLAALRARIQTFDATRVPYTSEELALFSTPGTTLMASA
                     HSGSRKTIKEPSGNAEVLLAEGRRYFVAHEFDKAEAKYAEALKLDPKNVGTLSDLASI
                     QLEEGRAEDAEKNLKAALAIEPEDDYSLFVLGQLKFRQKSYDEAFDALSRAAQLNPQN
                     ARIQNSLGLTLSEKGYRGPAETAFRKAIQLDPGFADAHINLAVVYATQQPPLLELARW
                     HYQKALATGHAANTVLEKLLEQGGQSVAK"
     gene            2574..3188
                     /locus_tag="VG754_03690"
     CDS             2574..3188
                     /locus_tag="VG754_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWF18340.1"
                     /translation="MSSFLNQHVLVLNRLWQAVNICTARRALTLLFEGHAQAVLGGND
                     GSFQTFSFDQWQDFSEQNPHPESIHTISFRIRVPRVILLVMFDRLPKKEVKFTRHNIF
                     ERDNNKCQYCGQTFDRKDLNLDHVIPRDRGGPTTWENIVCSCIPCNTRKANHTPQEAG
                     MHLIRKPKRPKWRPFVQINIGMHHHESWKHFIDLAYWNVELGEE"
     gene            3264..3713
                     /locus_tag="VG754_03695"
     CDS             3264..3713
                     /locus_tag="VG754_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18341.1"
                     /translation="MAKTPLLLFSFGAGRLNQFPELRVLLQGLVLIDLQPGAEVKILE
                     RMPAQNPMNDQPQIVPLEINPVIPHPEPMQNTTRPLEFAELVHFCVKNLLRQAAKFAE
                     DVKLQFLGHARQLSRACRIKDDLKWEHLNENSGIQREFHNYWKDYQG"
     gene            complement(3791..3865)
                     /locus_tag="VG754_03700"
     tRNA            complement(3791..3865)
                     /locus_tag="VG754_03700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3830..3832),aa:Glu,seq:ctc)
     gene            complement(4004..4642)
                     /locus_tag="VG754_03705"
     CDS             complement(4004..4642)
                     /locus_tag="VG754_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18342.1"
                     /translation="MKKALILNLFIALGALSSMAQGVVNANNVGSGVSIYIQDTTGGI
                     DGGAAVMIGIPAATAGFNGAGPGQVSFSLYAAPLGTSLASLEATTPIWTGANPNSTAP
                     SAQGAVVPGSEFILPSPFDGSAPVEFIFQGTATINGTTVYDGWSSEGVVTPYPASKDD
                     PAPTIWGTGIGQISSMVLTPASAPEVSSTLVLSSLSAIGIFLFQSRRKMIRD"
     gene            complement(4663..5280)
                     /locus_tag="VG754_03710"
     CDS             complement(4663..5280)
                     /locus_tag="VG754_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWF18343.1"
                     /translation="MKQPLYLAAIVLAGTLSSMAQGVVNPNNDGSGVLIQIEDASGNL
                     NGGIPVAIGTPATADGFIGAGPGQIAMELYAAPTGTSLQNLEASTPIWTGFNSLTTIP
                     GFQGTVFPGNSFILPTTPGFDGTANVEFIFSGQATVNGTAIQAWSGVGVVEPAQPGSI
                     SPPPQIWGGPDGIPSMIFIVPEPSTIVLSGLGAAALLLFRRRRNF"
     gene            complement(5298..6077)
                     /locus_tag="VG754_03715"
     CDS             complement(5298..6077)
                     /locus_tag="VG754_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18344.1"
                     /translation="MKARSRLRMVAIVAAGVISAVAQLPSFSQTTNLSITVVPGYNLI
                     SLPLKNTNDSINTVLTNTYYNGNPQVTPGGCSIFTWDPVHARFNPAIYAGGDGNWYNE
                     DFTLATLPLPPGLGFFFQLITPSQQQGSGDPPPFVTNLTIVVTGKILTGTNSYPVTSG
                     YNFYGNFEPTTNDICKNGFPVVDGSDLMTWNGYGYGQALYGLGTNDSAPDLSGNPGQY
                     PGFIDQDFTQIVPVTPPVGSAFLYYYVGPNTNWTQIYSVGP"
     gene            complement(6387..7322)
                     /locus_tag="VG754_03720"
     CDS             complement(6387..7322)
                     /locus_tag="VG754_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18345.1"
                     /translation="MNSGISKFFCLCGLIAFASGARANVSDATAGTDMSGVSTDQPYS
                     AIWVRNVFDLKPKDPPPSTAPTETAPPSNIKLMGIYTIFGKRALLGVLEGQMPGQAAK
                     KEECYNMSEGERRGSLEILEINPNARTVKIKNDQIVSTITFETNKPAGATMPNGRPTG
                     FGGGMGQPAFHPTGMPVPGPLPSRMTRPPSTQNNFQPSAAGYQSGYTGANTGTTLNNL
                     FNQPQNHQNAPAPTSDLTAEEQVAAVLMNQKLHENDPVYPPAMPPIPGLNLGGTGTGA
                     ENNSTTTPANNPTLPPWLRNRTIPSFGGAPPPPAP"
     gene            complement(7395..9509)
                     /locus_tag="VG754_03725"
     CDS             complement(7395..9509)
                     /locus_tag="VG754_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18346.1"
                     /translation="MSGKKETIRLALICGALMLLTLIVFWRVLGCDFLYYDDNTYVTG
                     NSHVQEGLTWAGIKWAFQASAASNWHPLTWISHMLDFQLYGLKPGGHHLTNLLFHTAN
                     VLLLFIWLNRLTGAVWKSALVAALFAWHPLHVESVAWVAERKDVLSTFFWLLTLIAYT
                     ERSNAQGRKAHIFYGLSLFFFVLGLLSKPMLVTLPFVLLLVDYWIAESEKTQDISRLP
                     WVRWISEKIPFFALTAASCIATFVAQQEGGAVRSFKVFSFEQRIANAIMSYARYLNKT
                     FWPHHLAALYPHPVNWPFLQVLEAAVVLLALSYWVMREMPKWPWVAVGWFWFLGTLVP
                     VIGLVQVGVQSLADRYMYVPSIGLFIIVAWGLEQCVRSLKYRSLAVGATGVALCSCMF
                     LTWRQIGFWKNSETLFKRDIEITGYNFIACSDLATFLLDHNKGDKALFYYEKALAAAP
                     NFALAEIGLGMAQLKLGRTQAASEHLLKAAKLAPDNTDVHLNLALAYEKLDQQTSAIE
                     QFRIVNTLRPSLTARKQLGNALAKSGDLTGAIAEFTAVAEANPKDLEAQSQLGCFFFQ
                     ENRLSDAIDHYRQAIRLYHEALQERPDSPILLNNFAWLLATNPNAEIRNGPEAVRLAQ
                     KACELTRYETPVLLGTLAAAYAEASQFSNAVATATQAHDLAQAKNQKRIADRNAELLE
                     LYRANQPYHESGRTNNAMPKVR"
     gene            complement(9724..>11214)
                     /locus_tag="VG754_03730"
     CDS             complement(9724..>11214)
                     /locus_tag="VG754_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18347.1"
                     /translation="AMDSVVTALFQRGAMADLEAVSLWDRFANSCVSYFRYISKLFVP
                     VKLAVIYPFTGWPIWLVVLCAAGLIGVSWLAIRARKQKPYLPVGWFWFLGTLVPVIGL
                     VQVGVQSMADRYTYLPLIGLFVVIAWGGADLAGKLSRQKITAGILAVTGLIVCALLTS
                     VQLQFWQDSRTLYERTLAVTEDNYLACSNMGFLLSREGKMEAAIDYCRKALKIKPRFS
                     VTIFNLGFILESQGRLEEARQEFEDGLQLVPDKVEARVHLARILGLEGKEDEAIEQFT
                     EALQTDPKEFEAHLNLGRLLARKKRLGEAVDQFQTVLSIKPGWPPALKSLGDVFAEQG
                     DVAGALAKYSEALKADPDDAETHSQFAALLSSQHKMTEALVHYREAVRLSPDSPVTLN
                     NLAWVCATDPDPQIRNGAEAVRLAEKACQLTRHEVPVLLGTLAAAYAENRQFTNAVAT
                     ATQARDLALAKKDQTVADRNAELLRLYQAGKAYHQEEKLAPNDHRN"
BASE COUNT         2571 a         3111 c         2902 g         2630 t
ORIGIN      
        1 ggtgcccagg gcaagctggc cgttgatgat tggaatgctg agattgacga cggtcaatgc
       61 gctgcgcacg tgcgccggca tgtcgtcttc gccttcgctg gtatgttgaa aaagcgggtc
      121 gccgtcggga accagccgcg aaaaaaatcg ctccaaatcg cgccggactt cgggatctgc
      181 attttcctgg atcaataacg acgcggaagt atgccgcacg tgcaaggtca acaggccatt
      241 cttaatgccg ctctcatcaa tccacgctgc aacagcccgc gtgatttcat agaacccgcg
      301 cctgcgcgtg ctgactgtga attcatgggt gccttgtcga agcattttta aagttactgt
      361 tgaagccact gattaaacac agatcaaaca cggatggaga tcaactccat tttggatcca
      421 tggcccaaca tatcacaatt cacttcgcaa cgctctggcc gccctgttcg agcaattttt
      481 ccagcacggt atttgccgca tggcccgttg ccagggcttt ttggtaatgc cagcgcgcca
      541 gttcgagcaa tggcggttgt tgcgtggcat agaccacagc caagttgatg tgcgcatccg
      601 caaagcctgg gtcgagttgg atggctttgc gaaatgccgt ttccgccggg ccgcgatacc
      661 ctttttcgct caaagtgagg ccgagtgaat tttggatgcg cgcgttctgc ggattgagtt
      721 gcgccgcgcg gctcaatgcg tcgaacgctt cgtcataact tttctgacgg aatttcaatt
      781 gcccgagaac aaacaggctg tagtcgtcct ccggctcgat ggccagcgcc gccttcagat
      841 ttttttcagc atcctccgca cgtccttctt caagttggat ggacgccagg tctgagagcg
      901 tgcccacatt tttgggatcc aatttcaacg cctcggcata tttggcctcg gccttgtcaa
      961 attcatgcgc gacaaaataa cgtctgccct cggcaagcag cacctcggca tttccagaag
     1021 gctccttgat cgtctttcga ctgccgctat gcgcggaggc catcagcgtc gttccaggag
     1081 tgctgaaaag ggccagttct tcggaagtat aaggcacgcg tgtcgcgtcg aatgtctgga
     1141 tgcgcgcgcg caacgctgcg agttgccgcg acatttcttc cagacgcgcc gccatttctt
     1201 ttcccttcct gcgaccggct gcatcgcgcg tcgcggcttc caaacttttc tgcaattcat
     1261 cccgttgcgc ttcaagttga gcaatcttgc ttgccatcac gctgtccgac atctccggtg
     1321 aagttgaatt cgtctccgca atggaaactg cgggagcgcc attcgtgctg gaatacgccg
     1381 gaacaagggg cgtgatagag gacagttgcc tgacccggtt ttccagcgca attttttcca
     1441 ggcgcaaaat ttccttgtcg gattgcatgg cggcaatttg cgcctgcgct tcctgcaatt
     1501 ttttgtccaa cgcttcggac ggcgttgccg gcgcgccctt cttcaattgg gcgacttgtt
     1561 ttttgagcag ttcgttttcc tcgcgcaacg cggctgccgc cggatccgct acgttcaaag
     1621 ttttcatccg cgtttgtaac gcctcttttt cctgcgccag cgttgcattt gcctctgtca
     1681 aggccgatac cttgcggttc gcttccgcca gcgcttgttt ggttttttcc aaatccgccg
     1741 gagcagccgc cgcaagcgca gaattggtct tcgcgctttc aaggttggcc ttcagcaact
     1801 cattttcctt ttgcaaactg gtgatttgct cgcgcgcctt ggccaattcc tgcggatcaa
     1861 cttccgccgg acgggccgca agcgcttcct tcaattttgc ttccagcaaa gagcgatcgg
     1921 cttctaaatg gcgcacctgg tcctccaagg ctttcatctt gttttccatt tcggcagacg
     1981 gaggcggtgc cgttggattg gcaggcgctt tgttctcagg cgcagttgcg ggggcaggac
     2041 tcgtcggcgc ggctggaacc ggcgcgggcg ggggtgcatt ggttgctgct ggaggttgcg
     2101 cgttggtggc cggttttggt gcttgagacg aaagctgctc gattttgttg gcgagataat
     2161 tgagccggaa cttgaccact ttcaaatacg catcgggata ggctttctgg aaacgcttca
     2221 atgccccttg cgcctcgaca tatttggtga tggccgcacc ccaatcgccc ttttcggtga
     2281 gcgcgtcccc ctgctggatc atcccgtaaa tcgtcacata atcgtcatcc ggcccttccg
     2341 ccctggcggt cggcagcatg accaataccg acaaaaacag cactgcgaga aaccttttca
     2401 ttgaagcatt agtaacagaa ttgaaaacgg ctggcaatca taagtcaccc attgaggtgg
     2461 agaacaggca gttgcatcca aaatccaatg tttgaaattc aaatcagcgg gcgccccgca
     2521 ttgacacctc cttgagcctt atgttaaatt ccgcgccaat aacgcatttg gttatgagct
     2581 cgtttttaaa ccaacatgtg ctggtcttga atcgcctttg gcaggctgtg aacatctgca
     2641 ccgcccgtcg cgccctcact ctgctcttcg aaggccatgc gcaagccgtc ctcggcggaa
     2701 acgacggctc gttccagacc ttcagcttcg atcaatggca ggatttttcg gagcaaaatc
     2761 cgcacccgga aagcatccat accatttcat tcaggatacg cgtgccgcgt gtcatcctgc
     2821 tggtcatgtt cgaccgtttg ccgaaaaaag aggtgaaatt cacccggcac aacattttcg
     2881 agcgtgacaa caacaagtgc caatattgcg gccaaacttt cgaccgcaaa gatttgaatt
     2941 tggaccacgt catcccccgc gatcgcggcg gcccgacaac ttgggaaaac atcgtttgct
     3001 cctgcattcc ttgcaacacc cgcaaggcaa accacacgcc ccaggaagcc ggaatgcacc
     3061 tcatccgcaa acccaagcgg cccaagtggc gtcctttcgt ccaaatcaac atcggaatgc
     3121 accatcacga aagctggaag catttcattg atctggcgta ttggaacgtg gaattgggag
     3181 aggaataact ttccattcgg gaagtttcaa caacacctgc cgctcctcat tggatcacag
     3241 tcgttccgga aggaaagccg gcaatggcta aaacgccact actgcttttt agtttcggtg
     3301 cgggccgcct caatcaattc ccagaacttc gggttctgct gcagggcctc gtcctcatcg
     3361 atcttcaacc cggtgcggaa gtaaaaatcc ttgagcgtat gccggctcaa aatccgatga
     3421 acgaccagcc ccaaatcgtg ccgctcgaaa taaacccggt aatcccccac cctgaaccga
     3481 tgcaaaatac gacccgtccg ctcgagtttg ccgaactggt ccatttctgt gttaagaacc
     3541 tgttgcggca ggccgcgaaa ttcgccgagg atgtaaagct gcaattcctt gggcatgcgc
     3601 gccaactcag ccgcgcttgt cggattaaag atgatttgaa atgggagcat ttgaatgaga
     3661 attccggtat ccaacgcgaa tttcacaact actggaagga ttatcagggc tgatttaatt
     3721 ggtgtcaatt cgtgaaattc gtgtcggaat tgccggccct caactctcaa ccttcatccc
     3781 tcaaccattt tggtagcgcg tacgggaatc gcacccgtct ttcagccttg agagggccgc
     3841 gtcctaactg atagacgaac gcgcctgcca atttcctttt catgctaccc aacttcggac
     3901 cgacgggcaa attcaaaatg caaaaggaaa aattcaaaat tctggcgcgg acgtcacaag
     3961 tgggcaaaaa agcaaaaacc gttcttcgcg ttctggggca gaatcaatcc cgaatcattt
     4021 tccttcgaga ttgaaaaaga aatatgccaa tcgcgctcag agagctcaaa accagcgtgg
     4081 aagacacctc gggagcgctg gctggcgtca gcaccatgct cgaaatttgg ccgatgcctg
     4141 tgccccagat tgtaggagct ggatcatctt tcgacgcggg ataaggtgtg acaacgcctt
     4201 cgctggacca tccatcgtaa accgtggtgc cgttgattgt cgctgtgcct tggaatatga
     4261 attcgaccgg cgcggatcca tcaaagggtg aaggaagaat gaactctgag ccgggaacca
     4321 ccgcgccttg ggcactcgga gctgtgctgt taggattggc tcccgtccat atcggtgtgg
     4381 tcgcttccaa cgacgccagc gaagtgccaa gcggcgcggc ataaagagaa aatgatacct
     4441 gtcctggacc ggcgccgttg aaacctgcgg ttgccgcggg aatgccgatc atcactgctg
     4501 cgccgccatc aattccaccg gtggtgtcct ggatgtagat tgacacgccc gacccgacgt
     4561 tgttggcgtt cacgacgcct tgtgccatcg aactcaacgc gcccagcgcg atgaacaaat
     4621 taagaatgag cgcttttttc atatttacgt ctgtgtgtat ctttaaaaat tacgacgacg
     4681 acgaaacagc aacagcgccg ctgcgccaag gccgctgagc acgatggtgg acggctccgg
     4741 aacaatgaat atcatgctcg gaatgccatc cggcccgccc caaatctgcg gcggtggaga
     4801 aatcgaaccc ggttgggccg gctcaacaac gcccaccccg gaccaggctt gtatcgccgt
     4861 gccattcacc gtcgcctgac cggagaaaat gaattcaacg ttagccgttc catcaaatcc
     4921 gggtgtcgtc ggcaaaataa aggagttccc aggaaaaacc gtgccttgaa accccggtat
     4981 cgtcgtcaac gaattgaacc ctgtccatat cggcgtgctc gcctccaagt tttgcaatga
     5041 agtcccggtg ggcgcggcgt aaagttccat ggcgatctgt cccggacccg cgccgataaa
     5101 accgtccgcg gtcgccggag tgccgatggc aactggaatt ccaccgttca gattcccgga
     5161 ggcatcttcg atttgtatca aaactcctga accatcgttg ttgggattga cgactccctg
     5221 ggccatcgag ctcaatgtcc ccgccagaac gattgctgcc agatagagtg gttgtttcat
     5281 gacggcgcag tgttccatca aggcccgacc gaataaattt gagtccagtt cgtgttcggc
     5341 ccgacatagt aatacagaaa tgccgagcca acaggcggag tcacaggaac gatctgtgtg
     5401 aaatcctggt caatgaatcc cgggtattgt ccggggttgc cggacaaatc aggcgcgctg
     5461 tcattcgtgc ccaacccgta gagcgcctgg ccgtaaccat aaccgttcca agtcatcaaa
     5521 tcggaaccgt caaccaccgg aaagccgttc ttgcaaatgt cattcgtcgt cggctcaaaa
     5581 ttgccgtaga aattatagcc ggacgtgacg ggataactgt tcgtccccgt caggatttta
     5641 ccggtcacaa caatcgtcag gttcgtaaca aagggcggag ggtcgccgga accctgctgc
     5701 tgggatggag tgatcaattg aaagaagaaa cccaacccgg gtgggagcgg cagcgttgcc
     5761 agggtgaaat cttcattata ccagttgcca tcgccgccgg cataaatcgc cgggttgaac
     5821 cgggcgtgaa ccgggtccca ggtaaaaatg gagcaaccgc cgggagtcac ttgaggattg
     5881 ccgttgtagt aggtgttggt gaggacggtg ttgatgctgt cgttcgtgtt cttaagcggc
     5941 aaactgatga ggttgtaccc cggcactacg gtgatgctca ggttggtggt ttgcgaaaaa
     6001 gaaggcagtt gcgcaaccgc agagatgacc cctgctgcga cgatggcgac cattcgcagt
     6061 cgtgaacggg ctttcatgat ttccttctga ttattggaat aaaatggcag tcttcgatga
     6121 aaaaatcctc atcggtcatg aatgcacacg gattgattga tgctaggttg ccggttttta
     6181 aagtcaatag aaattgaaag aacttgttca cattttattt cccgtacccg tacggagatc
     6241 tgcgctttgt cactaaataa aaaagccgtc cttgcagacg gctttgtcaa gtcactctgt
     6301 aattgatact tgcgatgggc agaacccgcg ggtcgaaaat cacgcgcccg ttaggagctt
     6361 tttgcaaaga aagccgaagc aactgttcag ggtgccggcg gcggcggtgc accaccaaag
     6421 gacgggattg tcctatttct caaccatggc ggaagtgttg ggttgttggc aggagtcgtt
     6481 gtagaattgt tttccgcgcc tgttccagtt ccacccagat tcaatccggg aatgggtggc
     6541 atggcgggcg gatagaccgg atcattttca tgtaacttct gattcatcaa cactgcggca
     6601 acctgttctt ccgctgttaa atcagacgtt ggtgctggcg cattctggtg gttctgaggt
     6661 tgattgaata gattgtttag tgtggtgcca gtatttgcac ccgtatagcc ggactgataa
     6721 ccagcagcgc tgggttgaaa attattttgt gtggatggtg gccgcgtcat gcgactgggc
     6781 aacggcccgg gcacaggcat gccggtcgga tggaacgcgg gctgccccat tccaccccca
     6841 aatccagtgg gacgcccatt gggcatcgtc gcgccagccg gcttgttcgt ttcgaaagtg
     6901 atcgtggaga caatctggtc attcttgatc ttcaccgtgc gggcattggg attgatctcc
     6961 aaaatttcca gcgaaccacg gcgttcgcct tcgctcatgt tgtaacattc ctctttcttg
     7021 gcggcctgac ccggcatctg cccttccaag acgcccaaca aggcgcgctt gccaaaaatt
     7081 gtataaatgc ccatcaactt tatattcgat ggcggagccg tttcggtcgg agcggtgctc
     7141 ggaggagggt ccttgggctt caaatcaaat acgttgcgca cccaaatggc gctgtaaggc
     7201 tggtctgtcg agacaccgga catgtccgtc ccggcggtgg catcgctgac attcgcccgg
     7261 gcccccgagg cgaaggcaat caacccgcac aggcagaaga acttagatat gccgctgttc
     7321 atggtatagt attataggcc atcaatataa caccacaagt gttttttagt tccggtttgc
     7381 gatgccgaac ttcatcaccg cactttcggc attgcattat tcgtcctccc gctttcgtga
     7441 tacggctggt tggcgcgata tagttccagc agttccgcat ttcggtcagc gattcgcttc
     7501 tggtttttgg cctgggcgag atcatgtgcc tgagtggccg tcgcgaccgc attgctgaac
     7561 tggctggctt cggcgtaagc ggccgccagc gtgcccagca aaacgggtgt ttcataacgt
     7621 gtcaattcgc acgccttttg cgccaaacga acggcctcgg ggccgttgcg gatttccgcg
     7681 ttgggatttg tggcgagcag ccatgcgaaa ttattgagaa gtatcggcga atcgggacgt
     7741 tcctgcaatg cttcgtgata aagccggatg gcctgccgat aatggtcaat cgcatctgat
     7801 agacggttct cctggaagaa gaagcatcca agctgcgatt gtgcttccag atctttggga
     7861 tttgcttcgg caaccgccgt aaactcggca attgcgcctg tcaaatcccc acttttcgcg
     7921 agcgcgtttc ccaactgctt tcgcgccgtc aagctgggac gcaaggtgtt cacaatgcgg
     7981 aattgctcaa tggcgctggt ctgttgatcc aatttttcgt atgccaacgc aagattcaaa
     8041 tggacatccg tattgtcggg cgcgagtttc gcggccttga gcagatgttc cgatgcggct
     8101 tgcgttcttc ccagcttgag ctgcgccatg cccaaaccaa tttccgcaag ggcaaaattc
     8161 ggcgcggcgg cgagcgcctt ttcataataa aacaatgcct tgtcgccttt gttgtggtcc
     8221 agaagaaaag tcgcaagatc tgaacacgcg atgaaattgt acccggtgat ctcgatgtca
     8281 cgcttgaaaa gagtttcgct gttcttccag aaaccgattt gccgccatgt taaaaacatg
     8341 catgagcaaa gagcgactcc tgttgctccc acggccaatg aacggtattt caacgatcgc
     8401 acgcactgtt ccagtcccca agccacgatg atgaacaaac cgatggaagg gacatacatg
     8461 taacggtccg cgagggattg aacaccaacc tgcaccaagc cgatcaccgg caccaaagtc
     8521 cccaaaaacc aaaaccatcc gaccgcaacc caaggccatt tgggcatttc tctcatcacc
     8581 cagtatgaaa gcgcgagcag caccaccgcg gcctccagca cctgcaaaaa aggccagttc
     8641 accggatgcg ggtaaagcgc cgcaagatgg tgcggccaaa acgttttgtt caaataacgg
     8701 gcatacgaca tgatcgcatt tgcgattcgt tgctcgaatg aaaaaacctt gaacgaacgc
     8761 actgcgccac cttcttgttg cgcaacaaaa gttgcgatgc atgatgctgc tgtcagtgca
     8821 aaaaacggga ttttttctga aatccaccgg acccatggca gcctggagat gtcttgagtt
     8881 ttttcagatt ccgcaatcca gtaatccacc agcaacaaca caaacggcag cgtcacaagc
     8941 atcggcttgg acaaaaggcc gagaacaaaa aaaaagagcg agagcccata aaagatgtgc
     9001 gcctttcgac cttgggcgtt ggatctttca gtatatgcta tgagcgtcaa cagccagaaa
     9061 aaggtgctga gcacatcctt tcgttccgcc acccacgcca ccgattccac gtgcagtgga
     9121 tgccacgcaa acaacgccgc cacgagcgca cttttccaaa ccgccccggt caaccggttc
     9181 aaccagatga aaagcagcag cacgttcgcc gtgtgaaaca gcaaatttgt gagatgatgc
     9241 cctcccggtt tgaggccata aagctggaaa tccagcatgt gcgaaatcca ggtgagcgga
     9301 tgccagttgc tcgccgcact ggcttgaaaa gcccatttta ttcccgccca ggtcaagccc
     9361 tcctgcacat gcgagtttcc cgtcacataa gtattgtcat cataataaag aaaatcgcac
     9421 cccaacaccc gccagaaaac aatcaaggtc agcaacatca aagcgccgca aatgagcgcc
     9481 aggcgaatgg tttctttttt accggacatg aaggcccaga atgaacgaaa tcgtttcgcg
     9541 catcaatcgg ttctttggct gttttatgtc gaatatgagc atcggttatt gcagcgactt
     9601 tccagagaaa gctgaccgat cacgcgctca tggcacggcg cgccggtgtt agaccaccaa
     9661 acgacgggat tgtcttgttt ctcaaccatg gaggaagtgt agtttggttg ttaggcatct
     9721 tttttagttc cggtggtcgt ttggagccaa cttctcctct tggtgatatg ctttgccagc
     9781 ttgatacaag cgcagcaatt ctgcattccg gtccgcgacg gtttggtctt ttttggccaa
     9841 ggcaagatcg cgcgcttgag tggctgttgc aacggcattg gtgaactgtc tgttttcggc
     9901 gtaagccgca gccagcgtac ccagcagcac gggcacttca tgccgtgtca attggcatgc
     9961 tttttctgcc aggcgcaccg cctcagcgcc gtttcggatt tgcggatcgg gatcagtcgc
    10021 gcaaacccat gccaggttat tcagcgttac tggtgaatct ggcgacaaac gcaccgcttc
    10081 ccggtagtgg accaacgcct ctgtcatctt atgctggctc gataacaacg cggcgaattg
    10141 cgaatgggtt tccgcatcat ccgggtctgc tttcaatgct tctgaatatt ttgccaacgc
    10201 cccggcaacg tcgccctgct ctgcaaaaac gtcccccaaa ctcttgagcg ccggcggcca
    10261 tccaggtttt atgctcaaga ccgtttggaa ttgatcgacg gcttcgccca ggcgtttttt
    10321 ccgggccagc aaacggccta gattgagatg cgcctcaaat tctttcgggt cggtttgcag
    10381 agcttctgta aactgctcga tggcctcatc ctccttcccc tccaagccta ggatccttgc
    10441 caaatggacc cgtgcctcga ctttgtcggg aaccagttgc aaaccgtcct cgaattcctg
    10501 ccgcgcttct tccagtcgtc cttgcgactc caaaatgaat ccaagattga aaatggttac
    10561 gctgaaccgg ggtttgatct tgagcgcctt cctgcagtaa tcaatggcgg cttccatctt
    10621 cccttcgcga ctcaggagaa atcccatgtt cgagcaggcc aagtaattgt cctccgtgac
    10681 tgcaagggta cgctcgtaca aggtcctgct gtcttgccag aattgcaact gcacgctggt
    10741 caaaagcgcg caaacaatca gtcccgtgac agccagaatt ccagcggtga ttttttgccg
    10801 cgacaatttg cccgccaaat cggccccacc ccaggcaatc accacaaaaa gcccgatcaa
    10861 tggcaaatag gtgtaacgat ccgccatgga ttgcacgccc acctgcacca atccaatcac
    10921 gggcacaagc gtccccaaaa accaaaacca tcccacaggc aaatacggtt tttgttttct
    10981 tgcgcggatc gccagccatg aaacaccgat caaccccgca gcgcacagga cgaccagcca
    11041 aatcggccat ccggtaaaag gataaatgac tgcgagtttc accggcacaa acagtttgct
    11101 tatgtaacgg aaataggaaa cgcagctatt ggcaaaacgg tcccacaagg aaaccgcttc
    11161 cagatcggcc atcgcaccgc gttggaaaag ggcagtcacg acactatcca tcgc
//
LOCUS       DASZMM010000147         8365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4998230, whole genome shotgun sequence.
ACCESSION   DASZMM010000147 DASZMM010000000
VERSION     DASZMM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4998230"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VG754_03735"
     CDS             complement(<1..784)
                     /locus_tag="VG754_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWF18348.1"
                     /translation="MKAEYLALFRSPFRFRRWGYVLLFSGLFLIFLLLVVTARALDHI
                     FFRRFRKQQVNEPVFIIAPPRSGTTLVQNLLSLDEERFLHLKMYQTIFPAVCFQRCID
                     AFCWMHDRLGAPFTKLLDFCERKWFGGWDDMHKMRLNQPEEDDALFLYAFESEAIFML
                     FPFVAELWEAGFPDALPPAERRKLMRYYRSCLQRHLYANGPRKTLLSKATQSSGAVES
                     LREEFPGAKFITIVRHPYRSVASHVSLFVPVWQAHSPDIAKDG"
     gene            832..1716
                     /locus_tag="VG754_03740"
     CDS             832..1716
                     /locus_tag="VG754_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HWF18349.1"
                     /translation="MGLNADTAIRVLTMTSLGGLLFTTGLRLTWLEIVNALRASRFGW
                     ILLVNFALVPALTFCVAHVFQFPSNIAIGMMLLAAAPFAPVVPVFTKMARGDMALAGA
                     LTALFPLLSAFLTPLVCEFALKPWLGTGPLKFNVLTILVVLVSTITLPLVIGVMFSHW
                     VPALGRKLLRPCEIFSEAAGALSLAFVSFVEFPAVRETGWKPLLAMAVVFELCLAIGY
                     FLGGSSAGARRVVALGTSNRNIALALLVGIESFPGTPILAGVVANGLLLIFLGLLHVA
                     YWRFWAEDSHEKAQKARK"
     gene            complement(1806..3233)
                     /locus_tag="VG754_03745"
     CDS             complement(1806..3233)
                     /locus_tag="VG754_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWF18350.1"
                     /translation="MESITEVFSEKCAGLDIGQLLEDHAGENYELHQRHINPAHVRTL
                     HTIGFDRCYVRAEGPYLWDVEGTKYLDMLSGYGVFGLGRNHPDVRRVLIDFLNADYPS
                     LVKMEAPLLAGLLAQELKKRMPNQLDMVFFTNSGTEGVETAIKYARCATGKPAIIHCQ
                     KAFHGLTYGSLSLNGDESFREGFAPFLTDCRMIPFNDLDALECELRRGDVAGFILEPI
                     QGKGVNIPSPGYLSEAAALCRKHGALFIDDEVQSGMGRTGKFLAIEHDGDVDPDIVIL
                     SKSLSGGYVPVGAVLCKKSIHDKVFSSMQRSVVHSGTFSQGSFAMAAGLASLDVLDRH
                     NLIENAAVWGDRIGEGLRAMIPRFEFLKEVRWRGLMIGIEFGPPRSFSLKAAWSVMHT
                     LDKSLFPQAAIIPLMDRHHIITQVAGHHIDVIKLLPPLVIGETDAQWFLNAFEDVLVQ
                     MHHFPGPAWEVLTDIGRLAVTARAR"
     gene            complement(3513..4595)
                     /gene="hypE"
                     /locus_tag="VG754_03750"
     CDS             complement(3513..4595)
                     /gene="hypE"
                     /locus_tag="VG754_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HWF18351.1"
                     /translation="MMGETIEKNGSEEGLVFGSCPMPIFDHKEIVLAHGAGGKLTQQL
                     IQDLFLPRFKNALLNPLHDGAMFSVNGARFAFSTDSFVVNPIFFPGGDIGELAVNGTV
                     NDLAMCGARPLYLSSAFILEEGFRMDELWRVIQSMQRAAEIAGVSLVTGDTKVVDRGK
                     GDKIFINTSGLGLIDPGVNIHPGRAQVGDKIILNGPIAAHGIAIMSVREGLEFESEIK
                     SDTAALNGLVQAMLATAPDIHVLRDATRGGITSALNEIAGQARVGIRIEETQIPIDEQ
                     VKGACEILGLDPLYVANEGKFVAFVPADFADRVLSVMREHPLGKEAVIIGEVVAEHRG
                     SVTMKTRIGGNRMVDMFAGEQLPRIC"
     gene            complement(4600..5217)
                     /locus_tag="VG754_03755"
     CDS             complement(4600..5217)
                     /locus_tag="VG754_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWF18352.1"
                     /translation="MNAISTANPAESHRDRILRKCAESVEMKQKFFHEHAGQIEKLAM
                     DMAARFQKGNKLLVMGNGGSLCDALHFSVEFTHPIIEKRPAFPVIPLMTDIATMTAIG
                     NDLDSSRIFINQLRLHGRPGDMALAVSTSGKSPNLIYALEEARKMEMLTIAWAGKDGG
                     RFPQLADYCFIVPSYSIHRIQEVHATLVHVLWDMIHIALGAEDVI"
     gene            complement(5214..6353)
                     /gene="hypD"
                     /locus_tag="VG754_03760"
     CDS             complement(5214..6353)
                     /gene="hypD"
                     /locus_tag="VG754_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HWF18353.1"
                     /translation="MKYLDEYRDEAVAKKLVDEIHRVVTKPWVLMEVCGGQTHSIVKY
                     GLDHLLPPEVELVHGPGCPVCVTSLEMIDKAHAIAKRPDVIFCSFGDMLRVPGSEADL
                     HILKSRGADIRVVYSPIDCLKIARANPDKKVVFFAIGFETTAPANAMSVWQARKQGIK
                     NFSILVSHVLVPPAMASILQSPLNRVQGFLGPGHVCAVMGYQEYEPICARYHVPIVIT
                     GFEPLDLLEGVLMTVRQLEKGEAKVENQYRRIVNREGNQPAQDVVNQVFEICDRKWRG
                     VGSISKSGYKLRYEFREHDAERIFETREIDTQESPMCISGLVLKGVKKPHDCPAFGRE
                     CTPMHPLGATMVSAEGACAAYHAYGRQRQLHQESESNHALKVGAT"
     gene            complement(6350..6652)
                     /locus_tag="VG754_03765"
     CDS             complement(6350..6652)
                     /locus_tag="VG754_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HWF18354.1"
                     /translation="MCLAIPGKVIAIDDDPQGVKMAKANFGGIVKQICLEYTPEVQLG
                     DYVLVHVGFALGRVDEEEARRTYQALEAIQQLGELDVAEVVSPEEPSLPETMGAAP"
     gene            complement(6654..>8365)
                     /gene="hypF"
                     /locus_tag="VG754_03770"
     CDS             complement(6654..>8365)
                     /gene="hypF"
                     /locus_tag="VG754_03770"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HWF18355.1"
                     /translation="GSIVALKGMGGFQLMVDARNETAVMRLRQRKRREEKPFALMYPS
                     LAALKHDCHVSELEARLLLSSQAPIVLFERNASSSTVSSSVAPGNPTLGAMLPYTPLH
                     HLFMRELGFPVVATSGNLANEPICIDENEALERLLGIADFFLVHDRPIVRHMDDSIVR
                     AMLGREMVLRRARGYAPSPIRLLPQPSTLNPQPFNVLALGAQLKSAVALSVGSDVFVS
                     QHIGDLEAKEACLAFGKVVTDLQQLYEIEPESVARDLHPDYFSSRFAQRIAAPAISVQ
                     HHYAHVLACMAENELEAPVLGVAWDGTGYGTDGTIWGGEFLLVNETGFERVAHLRPFK
                     LPGGDTAVRQPRRSALGLLYEIFGNGLFKEPELITLQSFTASELGLLRQALDKEINVP
                     VTSSAGRLFDAVASLIGLRQRVGFEGQAAMDLEFAVEEGIEESYPFEFQKNEASMIDW
                     EPMVLKILEEVRRKETVGVIAAKFHNTLTEMIIRVSHEIGFPRVVLSGGCFQNKYFTE
                     RTVRQLQQEGFRPYWHQRIPPNDGGIAVGQVVAALRTKNSADATQDRELSPVNSILEP
                     KEA"
BASE COUNT         1912 a         2384 c         2196 g         1873 t
ORIGIN      
        1 ggccgtcttt cgcaatgtcc ggcgagtgcg cttgccacac cggaacaaaa agactgacat
       61 gcgatgcgac ggaacgatac ggatggcgca cgatggtgat gaatttcgcg ccgggaaatt
      121 cttcgcgcaa cgattcgaca gcgccggagg attgcgtggc cttggagaga agcgtcttgc
      181 gcgggccgtt cgcgtaaaga tggcgttgca agcaactgcg atagtaacgc atcaatttgc
      241 gtctttcagc aggcggcaac gcgtccggaa agccggcttc ccaaagttcc gcgacgaacg
      301 gaaagagcat gaagatggct tccgattcga atgcataaag aaaaagcgcg tcgtcttcct
      361 cgggttgatt gagccgcatt ttatgcatgt catcccatcc gccgaaccat tttcgttcgc
      421 agaaatcaag cagcttggtg aaaggcgcgc cgagccggtc atgcatccag caaaacgcgt
      481 caatgcaccg ttgaaaacag accgcgggaa aaatcgtttg atacatcttg agatgcagga
      541 atcgttcttc atcgaggctg agcagatttt ggacaagcgt cgtgccgctg cgcggcggcg
      601 cgatgatgaa caccggctca ttgacttgtt gtttccgaaa tcgtcggaag aaaatatggt
      661 ccaacgcgcg ggccgtcacc accaacaaca ggaagatcag gaaaagtccg ctgaacaaca
      721 gcacgtaacc ccagcgccga aaacgaaacg gcgaacggaa caacgccagg tattccgctt
      781 tcaagaacag tttgtgattg aaatacatcc tgtgctttac tgctttgagt gatgggtttg
      841 aacgccgaca ccgcgatccg ggttttgaca atgacctcgc tgggaggatt gcttttcacc
      901 acgggcttgc gtttgacgtg gctggaaatc gtcaatgcgc tccgtgcaag ccggttcggc
      961 tggattttgt tggtgaattt cgcgctcgtc ccagcgctca cattctgcgt cgcgcatgtc
     1021 tttcaatttc cctcgaacat cgcgatcgga atgatgctcc tggccgccgc gccgttcgct
     1081 cctgtcgtgc cggtgttcac caaaatggcg cgcggtgata tggcgctcgc cggggcattg
     1141 accgcgctgt tcccgctcct ttcggcattc ctcacaccgc tggtttgtga attcgccctg
     1201 aaaccgtggc tcggaacggg gccgttgaaa ttcaatgtgc tcaccattct cgtcgtcctc
     1261 gtttcgacca ttacattgcc gctggtgatt ggggtgatgt tcagtcattg ggtaccggcg
     1321 cttggccgca aattgctgcg tccctgtgaa attttctccg aagcggcggg cgcgttgtcg
     1381 ctggcgtttg tcagcttcgt ggaatttcca gcagtgcgcg aaaccggctg gaaaccattg
     1441 cttgcgatgg cggtggtttt tgaactctgc cttgcgatcg ggtatttcct cggcggctcc
     1501 agtgcaggcg cacgtcgagt cgttgcattg ggaacgagca atcgcaacat cgcgctcgcc
     1561 ctgttggtgg gcatcgagag ttttccggga acgcccatcc tggcaggagt ggtcgcgaac
     1621 ggcttgttgt tgatttttct cggtctgctg catgtggcgt actggcggtt ttgggcggaa
     1681 gacagccacg aaaaagcaca aaaggcccga aaatgaattt ccaatcagcc caagacgaca
     1741 tgtcgctcgt tgacggacgg acggcagcac catttgtgct ttctttgcgt gtttgtggcc
     1801 cttccctaac gcgcccgggc agtcaccgcc agacggccaa tgtcggtcag cacttcccag
     1861 gcggggccag ggaaatgatg catctgcacg agcacatctt cgaacgcgtt caggaaccat
     1921 tgggcatccg tttcaccaat gacgagcggc ggcaaaagtt tgatgacatc aatatgatgc
     1981 ccggcgacct gggtgatgat atgatgccgg tccatcaacg ggatgatcgc cgcctgtggg
     2041 aagaggcttt tgtccaaggt gtgcatcacc gaccacgcgg ccttgaggct gaacgaacgc
     2101 ggcgggccaa actcaatgcc gatcatcaaa ccgcgccaac gcacttcctt gagaaattca
     2161 aatcgcggaa tcatcgcgcg cagtccttcg ccgatgcggt cgccccatac ggcggcgttt
     2221 tcaatcagat tatggcgatc cagcacatcc aacgacgcga gccccgcagc catggcaaaa
     2281 ctgccctggc tgaacgttcc agaatggacc accgaccgtt gcatggagga aaaaactttg
     2341 tcatggatcg atttcttgca gagcaccgcg cccactggca cgtatcctcc ggaaagcgac
     2401 ttggacaaaa tcacgatgtc cggatcaaca tcgccatcgt gttcaattgc caagaatttc
     2461 ccggtgcgac ccatccccga ctgcacttca tcgtcaatga acaatgcgcc gtgcttgcga
     2521 caaagcgccg cagcttcgct gagataaccg ggcgaaggaa tgttcacacc ttttccctga
     2581 ataggttcaa ggataaatcc cgccacatca ccgcgccgca actcgcattc gagcgcatcc
     2641 aaatcgttga acggaatcat ccggcaatcc gtcaggaacg gagcaaatcc ttcgcgaaaa
     2701 ctttcgtcgc cgttcaggga caaagagccg taggtgaggc cgtgaaatgc tttctggcaa
     2761 tgaataatgg ccggcttgcc ggtggcgcaa cgggcatatt tgatcgccgt ctcgacgcct
     2821 tccgtgccgg agttggtgaa gaagaccatg tcgagctggt tgggcatgcg cttcttcaat
     2881 tcctgcgcga gcaatcccgc caggagcggc gcttccattt tcacgaggct cgggtaatcc
     2941 gcattcagga aatcaatcaa cacccgccgc acatcgggat gattgcgccc aagcccaaac
     3001 acgccgtagc cagaaagcat gtcgagatat tttgttccct ccacgtccca aagatacggc
     3061 ccttcagcgc gcacgtaaca gcggtcaaaa ccgatggtat gcagcgtgcg gacatgggcg
     3121 ggattgatgt ggcgctgatg cagttcataa ttttcgccgg cgtggtcttc caaaagctgg
     3181 ccaatgtcca gcccggcgca cttctcgctg aacacttcag tgattgattc catagtggca
     3241 aagcattctt taatagagtc ccctattaga ggggtcatca caatttttta cccatagggt
     3301 gattccctaa ccaagatttt agaccataaa agccgtaaaa agttacacta tttcgattta
     3361 gcacgcatct tgccgtgtcg tcaatctctc gtgagcagca tgaaattagg ccgaaaaaga
     3421 agtaattgtc agaacagata tcaaccgttg aacacctcat gtcaaatttc gaaatcccga
     3481 aactcagagg gtgtgttcaa ccttttcctt tttcaacaaa tccgaggcaa ttgctcgcca
     3541 gcgaacatgt ccaccatgcg gttcccgccg atgcgagttt tcatggtcac gctgccgcga
     3601 tgttcggcga ccacttcacc gataatcacc gcttctttgc ccaagggatg ttcgcgcatg
     3661 accgataaca cacggtcggc gaaatcggcc ggaacgaacg cgacgaactt gccttcattg
     3721 gcgacgtaaa gcggatcgag cccaaggatt tcgcaagcgc ctttgacttg ttcatcaatg
     3781 ggaatttgcg tttcttcgat gcggatgccg acgcgagcct gccccgcgat ttcattgagc
     3841 gcgctggtga tgccgccacg ggtcgcgtcg cgaaggacat gaatgtccgg cgcagtggca
     3901 agcatggctt ggacgaggcc attcaaagcg gcggtgtcgc tcttgatctc gctttcaaat
     3961 tcaagccctt cgcggacgga catgatggca attccatgcg cggcaatcgg cccattgaga
     4021 atgattttgt cgccgacctg cgcgcgtccg ggatggatgt tcacgccggg atcaatcaat
     4081 ccaagtccgg acgtattgat gaaaatcttg tcacccttgc cgcggtccac cacttttgtg
     4141 tcgccggtga ccaaactgac ccccgcaatt tcagccgccc gttgcatcga ttggatgacg
     4201 cgccagagtt catccatgcg aaagccttcc tcaagaatga aagcggaaga aagataaagc
     4261 gggcgcgcgc cgcacatcgc cagatcattc accgttccat ttaccgcaag ttccccaata
     4321 tcgccgccgg gaaagaaaat cggattgacg acaaacgaat cggtgctgaa tgcgaaacgc
     4381 gcgccgttca ctgaaaacat tgcgccatca tgaagcgggt tgagcaaggc atttttgaat
     4441 cgcggcagaa aaaggtcttg gatcaattgt tgcgtaagct tcccacccgc gccatgggcc
     4501 agcacgattt ccttgtggtc gaaaatcggc attggacagg aaccaaatac caatccctct
     4561 tcagagccat ttttctcgat ggtttcgccc atcataaatt caaatgacat cctccgcacc
     4621 gagcgcgatg tgaatcatgt cccataatac gtgaacaagc gtggcgtgca cttcctggat
     4681 gcgatgaatg ctgtatgaag gcacaatgaa acaatagtcg gccagttgcg gaaaacgtcc
     4741 gccgtccttg cctgcccagg cgatggtcag catttccatc ttgcgcgctt cttcgagcgc
     4801 ataaatcagg ttgggcgatt tgccgcttgt gctgaccgcc agcgccatgt cgcccggtcg
     4861 cccatgcaaa cgaagttgat tgataaaaat gcggctggaa tcaaggtcgt tgccaatcgc
     4921 ggtcatcgtg gcgatatcgg tcatcaatgg aatcaccgga aacgccgggc gtttttcgat
     4981 gatgggatga gtgaactcga cgctgaaatg caaggcatca cacaaacttc caccattgcc
     5041 catgacgagc agtttgtttc ccttctgaaa tcgcgccgcc atgtccatgg cgagtttttc
     5101 aatctggccc gcatgttcgt ggaaaaactt ttgcttcatc tccacgctct cggcgcattt
     5161 tcgaaggatg cggtcgcgat ggctctcggc aggattggca gtggagattg cgttcatgtc
     5221 gcgcccacct tcaaggcatg gttcgattct gattcctggt gcaattgccg ctggcgtcca
     5281 taagcgtggt acgcggcgca ggctccttcg gcggaaacca tcgtcgcgcc gagcggatgc
     5341 atcggcgtgc attcacgccc aaaagccggg caatcatgcg gttttttgac gcctttcaac
     5401 accagcccgc tgatgcacat cggcgattcc tgcgtgtcaa tttcgcgcgt ctcaaagatt
     5461 cgctcggcat cgtgctcgcg gaattcataa cgcaacttat aaccgctctt ggaaatcgag
     5521 ccgacgccgc gccatttgcg gtcgcagatt tcgaacactt gattgacgac gtcctgagcc
     5581 ggttgattgc cttcgcggtt gacaatccgc cgatattggt tttccacctt cgcctctcct
     5641 ttttcaagtt gccgcactgt catcaacacg ccttccaaaa gatccagagg ttcaaagccg
     5701 gtgatgacaa tcggcacgtg ataccgggcg caaatcggct cgtattcctg ataccccatg
     5761 accgcgcaga cgtggcccgg accgagaaat ccctgcacgc gattcagtgg cgattgcagg
     5821 atggacgcca tcgctggcgg caccagcaca tgcgaaacaa ggatggaaaa attcttgatg
     5881 ccctgtttgc gcgcctgcca gacggacatc gcatttgccg gcgcagtggt ttcaaagccg
     5941 atggcgaaga aaaccacttt tttatctgga ttggctcgcg cgatcttcag gcaatcaatc
     6001 ggcgaataaa ccacgcgaat gtccgcgccg cgcgatttga ggatgtgcag gtccgcttcg
     6061 gagccgggca cgcgcaacat gtcgccaaag gagcagaaaa tgacgtccgg ccttttcgca
     6121 atggcatggg ccttatcaat catctccagc gaagtgacgc aaacgggaca accggggcca
     6181 tgaacgagtt ccacttctgg tggcaagaga tgatccagac catatttcac gatgctgtgc
     6241 gtctgcccgc cgcaaacttc catcagcacc cacggcttgg tgacaacccg atggatttcg
     6301 tccaccaatt tttttgcgac cgcttcgtca cgatattcgt cgagatattt catggcgcgg
     6361 ctcccatggt ttcgggcaga ctcggctctt ccggcgaaac gacttcggca acatccagtt
     6421 cgcccaattg ttgaatcgct tcgagcgcct ggtaagtgcg ccgtgcctcc tcttcatcca
     6481 cccggccaag cgcaaatccg acatgcacca aaacgtaatc gcccagttgc acctccggcg
     6541 tgtattcgag gcagatctgc ttgacgatgc cgccaaaatt cgctttggcc atttttacgc
     6601 cctgcgggtc gtcatctatc gcgatcactt ttccgggaat tgccaggcac atatcacgct
     6661 tcctttggtt caagaatgga attaacgggc gacagctccc ggtcttgggt tgcatccgct
     6721 gaatttttcg tccgcaatgc cgccacgacc tgcccgaccg cgattccccc gtcattgggc
     6781 ggaatgcgct gatgccagta aggacgaaaa ccttcctgct gcaattgtcg cactgtccgc
     6841 tcggtaaaat atttgttttg aaaacagccg ccgctcaaaa cgacgcgggg aaagccaatt
     6901 tcatggctga cgcgaattat catctcggtg agcgtgttat gaaatttggc cgcgatgact
     6961 ccaacggttt ctttacgccg gacctcctcc agaattttca agaccatcgg ctcccaatca
     7021 atcattgaag cctcgttttt ttgaaattcg aacggataag attcttcaat gccttcttca
     7081 actgcaaact ccaagtccat cgccgcctgt ccttcaaagc ccacacgttg ccgcaagcca
     7141 atcagcgagg cgaccgcgtc aaacaatcgt cccgcgctgg aagtcacggg aacattgatt
     7201 tccttgtcca acgcttggcg caacaaaccc agctccgatg ccgtaaaact ctggagcgtt
     7261 atcaattcgg gctctttgaa taacccattg ccgaaaattt cataaagcaa tcccagcgcg
     7321 ctccggcgcg gttggcggac ggcggtgtct ccgccaggta atttgaaggg gcgcaaatgc
     7381 gcgacccgct caaatccagt ttcgttcacc agcaaaaatt caccgcccca aatcgttccg
     7441 tccgtcccgt agccggttcc atcccaagcc actcccagca ccggcgcttc cagttcgttt
     7501 tccgccatgc atgccagcac atgcgcgtaa tgatgctgga cggaaatggc cggcgcggcg
     7561 atccgttgcg cgaatcgcga agaaaaataa tccggatgca aatcgcgcgc gacgctttcc
     7621 ggctcgatct cataaagctg ctgcaaatct gtcacaacct ttccaaaagc cagacatgcc
     7681 tccttcgcct ccaaatcgcc gatatgctga ctgacgaaaa catcacttcc aacgctgagc
     7741 gcgacagcgc ttttcagttg cgcgccaagg gcgagaacat taaatggttg agggttgagg
     7801 gttgagggct gaggaagcag gcggattggc gatggcgcat aaccgcgggc gcggcgcaat
     7861 accatttcgc gccccagcat cgcgcgcaca atggaatcat ccatgtgacg cacgatgggg
     7921 cgatcatgga ccaggaaaaa atcggcgatc ccaagcaagc gctccaatgc ttcgttttca
     7981 tcaatgcaaa tcggttcatt cgcaaggttg ccgctggtgg caacgaccgg aaatcccaac
     8041 tcgcgcatga agagatgatg caacggcgtg tatggcagca tcgcgcccag agtcgggttt
     8101 cccggagcga cggatgacga gaccgttgat gaacttgcat tgcgctcaaa cagcacaatt
     8161 ggcgcctgtg aagaaagcag caatcgcgcc tccaattccg atacatgaca atcatgttta
     8221 agagcggcca gtgacggata catcaacgca aatggttttt cctcgcgccg cttgcgttga
     8281 cgcaatcgca tcaccgccgt ttcgttccgc gcatcgacca tcaattgaaa tcctcccatc
     8341 cctttcaatg ccacgatgct gccgg
//
LOCUS       DASZMM010000148         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5267293, whole genome shotgun sequence.
ACCESSION   DASZMM010000148 DASZMM010000000
VERSION     DASZMM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5267293"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1839
                     /locus_tag="VG754_03775"
     CDS             355..1839
                     /locus_tag="VG754_03775"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWF18356.1"
                     /translation="MKGFGLFQRFCRSGISLVFLLTGIFISHAQTSGGPVGPPITPFQ
                     VFITSPQNDAAFPVYAKITISATATNSSSFINTMFFYSGTNLLKAWVIDPVGPGQSNY
                     SLTLSFDWTNVPAGAYTLTAVAHNTDGISVTSPPVNITVGPARPVVTIVATDPIASMA
                     PGDTGTFTLYRSGGTSNSLEVFYGIGGTASNGVDYVTINNNVVIPAGASSAPITITPI
                     KDPFPDPNETVALRLVLSPLGSPLPDYKIGAPSNAVVTILGTNSIPPIVSIIATDPIA
                     VEPGTNRIVFQPMTPTFTNYCTGTNTATFLVRRISPTATGIPPSNSNDVTVYYNISGT
                     ASNGVDYATLPGFVTIPAGKNYALITVDPLEDTDPSVSPPFSTVILSLVVPPTDTNVP
                     PPYILGWPNKAGAIILEEHLYPIGPPIGPIAGGPFPCAPFYFHCPATNGMNYCLEVSS
                     DLINWLPVCTNTVVKSSIHFVDPDACGCTNRYYRAVPVVATPLY"
     gene            complement(1844..>4088)
                     /locus_tag="VG754_03780"
     CDS             complement(1844..>4088)
                     /locus_tag="VG754_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF18357.1"
                     /translation="SDAYKDHFFMTDFRGAPVISGVHSFAVTPRGAGFEVVDHNRFWW
                     HILGTDCKFSPDGQLYVCDWVRGWPQSGLGRMYRAYDPERVKSPLVLETKKLIAEGME
                     KRPVAELEKLLGHADQRVRQEAQFELAARASAAMPISDEGRKCLDALTRTALGNSNQL
                     ARLHGIWGLGQIARKSKDALNPILPLLTDNDSEVRAQAAKVLGDDRVAGAFTGLTNSL
                     ADQNLRVRFFAAQGLGKLGNPAAVKPLLAMLRENLDQDVFLRHAGVMGLLGCADKAAL
                     LEAANSDWRSVRMAALLAMRRREMPEISMFLQDPDPLIILEAARAISDLPIKDALPDL
                     AAMIEHPTGTPMLDWRIINANFRVGGEKNATALAAFAASGSANEDLRADAIRFLAGWR
                     KPFPRDRITGLWRPLPARDEKIAADALRPGIAQLISSAPDTVRLMAILAARQMSLTEA
                     AQALFDTAMNTSAATRLRIEALKGLETFHDPRLADVVKMAVADSDENVRNEGNRLEAS
                     MKPENAVAVVATALDTGSVTEKQGALRMLDSIEGNAADKLIADWMGKLIAGTAPKEIQ
                     FELLQDAKSHRTSDLVKARLKEYQASQSTNDEFAGYRETLYGGDAAAGRKIFLERPDA
                     SCVRCHKVNGEGGQVGPDLTGIITRHDREYILESVLYPNKVIAPGFESVLVEMKAGQT
                     YAGIVKSQDDEYLNLLSIEDNAVVKLKKADIKSQVKGQSPMPEGMGTILSKQDLRNLV
                     EFLATLK"
BASE COUNT          843 a         1252 c         1073 g          920 t
ORIGIN      
        1 gtcaaggaag accggctgat gctcacacgc aataacgatt acatcatgct cggcggaaaa
       61 ttcccgcgcc tcaccaagcc cgatgccaca ggccgtttga aagaaatcaa aaagttgctg
      121 ctggcgctct agcgaaatct gtggctgaaa acgtgagttt tcagcagctt ggaaaaggta
      181 aaatccacgc tttcccgtga aatcattggc aatcagcgac tttcaaatct tgcaaaaaag
      241 ccctgtttct gcgataaatg agtggttcaa acgaaataat tcattggcca cattgcatcg
      301 gacgggtttg atgttcggag accgtcatgc atcgaggatg aaagaaaggt ttgtatgaaa
      361 ggtttcggat tgtttcaaag gttttgccgg agtggcatct cactcgtttt cttgctcacc
      421 ggcatcttca tcagccacgc gcaaaccagt ggcggcccgg tcggcccgcc aatcacgccg
      481 tttcaggtgt tcatcacctc tccccaaaac gacgccgctt ttcccgtgta cgcgaagatc
      541 accatcagcg cgaccgcaac aaattcttca tcctttatca acacgatgtt tttttactcc
      601 ggcacgaact tgctcaaagc gtgggtgatt gatcctgtcg gccccggcca gtcgaattat
      661 tcacttacct tgtcgttcga ctggacaaat gttccggcgg gagcttacac gttgacagcg
      721 gttgctcaca ataccgacgg catttctgtg acctcaccgc cggtgaacat caccgtcggg
      781 ccggcgcggc cggtcgtgac catcgttgcc accgatccca tcgcatcaat ggctcccggc
      841 gacaccggga cattcaccct ctatcgcagc ggtggcacga gcaattctct cgaagtcttt
      901 tacggcatcg gcggcactgc ctccaacggt gtggattatg tgacgatcaa taataatgtt
      961 gtaattccgg caggcgcttc gtcggcgcca atcaccatca ctcccatcaa ggacccgttc
     1021 cccgacccca acgaaaccgt cgccctgcga ctcgttctat cgcccttggg ttcacccctg
     1081 cccgattaca aaattggcgc tcccagcaac gcggtcgtca ccatcctcgg cacgaacagc
     1141 atcccgccta ttgtctccat catcgccacc gacccgatcg cagtggaacc gggaacaaat
     1201 cgcatcgttt ttcagccgat gacgccgacg ttcacgaact attgcaccgg cacaaacacc
     1261 gcgacttttt tagtgcggcg catcagcccc accgcaacag gcattccccc gtccaactcg
     1321 aacgatgtga ctgtttacta caatatcagc ggcacggcat ccaacggcgt cgattacgcg
     1381 acgctacccg gatttgtgac catccctgcc ggaaaaaatt acgcgctcat cacggtggac
     1441 ccattggaag acactgaccc gtctgtttcg cctccgttca gcaccgtcat cctttcgctc
     1501 gttgttccgc cgaccgatac aaacgtgccg ccgccatata ttctcggctg gccgaacaaa
     1561 gccggcgcca tcattctcga agaacatttg tatccgattg ggccgccgat tggtccgatt
     1621 gcgggcgggc catttccttg cgctccgttc tacttccatt gcccggcaac aaatgggatg
     1681 aattattgcc tggaggtttc aagtgatttg atcaactggc tccctgtttg caccaacaca
     1741 gtggtgaaaa gctcaatcca ttttgtggac cccgacgcgt gcggttgcac caaccgttat
     1801 taccgcgccg tgcctgtcgt tgcgacgcca ttgtattaag ccgttatttc aacgtcgcca
     1861 ggaattccac gagatttcgc aagtcctgct ttgaaaggat tgtgcccatg ccttcgggca
     1921 tcggtgactg gccctttacc tgcgatttga tgtcggcctt ttttaatttc acgaccgcgt
     1981 tgtcctcgat ggagagcagg ttcaaatatt catcgtcctg gcttttgaca atcccggcgt
     2041 aggtttgccc ggctttcatt tccaccagca cgctttcgaa tccgggcgcg atcaccttgt
     2101 tcgggtaaag gacggattca aggatgtatt cgcggtcatg gcgcgtgatg atgccggtga
     2161 ggtcgggacc cacctggccg ccttcgccgt tgaccttgtg gcaacgcaca caggaagcat
     2221 cgggccgttc gaggaaaatc ttccggcccg cggcggcatc accgccgtaa agcgtctcgc
     2281 gataacccgc aaattcatcg ttcgtggact ggctggcctg atattccttg agccttgctt
     2341 tcacaagatc ggaagtgcga tgacttttag cgtcctgaag caattcgaat tggatttctt
     2401 tcggcgctgt tccggcgatc aatttgccca tccaatccgc gatcaatttg tccgcggcgt
     2461 ttccctcgat tgaatcgagc atccgcaacg cgccctgttt ttccgtcacg ctcccggtgt
     2521 ccagcgcggt cgcaaccacc gccacggcat tctccggctt catgcttgcc tccagccggt
     2581 tgccttcgtt ccgcacgttt tcatcgctgt ccgcgaccgc cattttgacc acatcggcga
     2641 ggcgcggatc gtgaaacgtt tccaaaccct tcaaagcttc gatgcgcaat cgcgtggcgg
     2701 cgctcgtgtt catcgccgtg tcaaaaagtg cctgtgccgc ttccgtcaag gacatttgcc
     2761 gcgccgcgag aatggccatc aatcgaaccg tgtccggcgc tgagcttatc aattgcgcga
     2821 tgcctggccg caatgcgtct gccgcgatct tttcgtcccg ggcaggcagc gggcgccaaa
     2881 gcccggtgat gcggtcgcgc ggaaacggtt ttcgccagcc ggccaaaaag cggattgcat
     2941 cggcccgcaa atcttcgttc gcgctgccgg aagctgcgaa cgccgccagc gctgtcgcat
     3001 ttttttcgcc gccaacccgg aagttcgcgt tgatgatgcg ccagtcgagc atgggcgtgc
     3061 cggtgggatg ttcaatcatc gcagccaaat cgggcagggc gtccttgatg ggcagatcac
     3121 tgatggcgcg cgcggcttcg aggatgatca acgggtcggg gtcttgcagg aacatcgaaa
     3181 tttcaggcat ctcccttcgc cgcatggcca gcagcgccgc catgcgcacc gagcgccaat
     3241 cggaattggc agcttcgagc aatgcagcct tgtccgcgca tccaagcagt cccatcacgc
     3301 ccgcgtgacg caggaaaaca tcctggtcga ggttctcgcg caacatcgca agcaacggtt
     3361 tcaccgcggc aggattgccg agcttgccga gaccctgcgc ggcgaaaaag cggacgcgca
     3421 aattttgatc ggcaagcgag ttggtcaaac cggtgaacgc gcccgcgacg cggtcgtcgc
     3481 ccaaaacttt cgcggcttgc gcgcggactt cggaatcgtt gtcagtgagg agcggcagaa
     3541 tgggattgag tgcgtctttg gacttgcgcg cgatttggcc gagcccccag atgccatgca
     3601 aacgggcgag ctgattgcta ttgccgagcg cggtgcgcgt caaggcgtca aggcatttgc
     3661 ggccttcgtc ggaaatcggc attgcagccg aagcgcgcgc agccagttca aattgcgcct
     3721 cctgccgcac ccgctggtcg gcatggccca aaagtttttc cagctctgcc acaggacgtt
     3781 tttccattcc ttccgcgatc aactttttcg tctccagcac gagcggactt ttgacccgtt
     3841 ccggatcata agcgcgatac atgcggccca agcctgattg cggccagccg cgcacccaat
     3901 cgcagacata caactggccg tccgggctga atttgcaatc ggtgccgagg atgtgccacc
     3961 agaaccggtt gtggtccacg acttcgaaac ccgcgcccct gggagtgacg gcaaacgaat
     4021 gcacgccact gatgaccggc gcgccgcgaa aatcagtcat gaaaaaatgg tccttatacg
     4081 catcggaa
//
LOCUS       DASZMM010000149         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_330004, whole genome shotgun sequence.
ACCESSION   DASZMM010000149 DASZMM010000000
VERSION     DASZMM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330004"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1202
                     /locus_tag="VG754_03785"
     CDS             9..1202
                     /locus_tag="VG754_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HWF18358.1"
                     /translation="MFGIEHIQLAEDVIQEALVRALQTWPYYGIPKNPSAWLMRVARN
                     LALDVVRRQKTFRDKEAEIIGQTEDGSFSSDEPVFSEHEMKDDVLRMMFVCCHPVIPR
                     EAQVALALKTLCGFNTAEISKAFLTTEAATAKRLVRAGQKIREARVPFEIPAGAESSQ
                     RLDAVLQTLYLLFNEGYKASSGSKLVREELCKEAIRLASLLAEHPVGNRPQTHALLAL
                     MFLNTARLRGRMDDEGNLLRLKEQDRGRWDQTLIARGIFHLAQSASGEEISEYHLQAG
                     IAAVHCAAKDYESTDWPQILSLYDRLIEFDDSPVVALNRAVALAKVHGAQAGLDAIAA
                     IPDREKLEEYYLLYAVLGEFATQLNDQQSAAAHFQKSLQLAELKSEKAFLEHRLRICE
                     EQISL"
     gene            complement(1309..>2251)
                     /locus_tag="VG754_03790"
     CDS             complement(1309..>2251)
                     /locus_tag="VG754_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF18359.1"
                     /translation="RFKDRENFWATLWAVIFAICQAKKFRERKPDVLHGVRATGPATA
                     AAILSRLCGIPFTFGAHAYDIYRHSGDAFLKPKLHSARFVHTTTAVAEKYLRERGGGA
                     EIKIVLARRGLESLPDKVSRATCYRAIALLSVGRLVPKKGHVHQIAACALLKSWDVPF
                     VARIIGDGPLRGELQQAIQHAGLQDCVTLCGATPPDAMDEAYYWADIFWHTGVVDAEG
                     DRDGLPNVIPEAFAHSLPVIASNLPGNLEAVADGINGLVVDATNAEQLAQAVKRLAED
                     SMLRKKLGANGLRWVEQNFLISRNAEILAGAFQSCVR"
BASE COUNT          485 a          648 c          641 g          477 t
ORIGIN      
        1 tcacgcgcat tttcggcatc gagcatattc aactcgccga ggacgtcatc caggaggcgc
       61 tggttcgcgc gctccagacc tggccttatt acggcatccc gaaaaatcct tccgcgtggt
      121 tgatgcgcgt cgcgcgcaat ctcgcgctgg atgtcgttcg gcgccaaaaa acttttcgcg
      181 acaaggaagc agaaatcatc gggcagacgg aagatggttc tttttcgagt gatgagccgg
      241 ttttttccga gcacgaaatg aaggacgacg ttttgcggat gatgttcgtc tgttgtcatc
      301 cggtgattcc gcgcgaggcg caggtcgcgc tggccctcaa aactctttgc gggttcaaca
      361 ccgccgagat cagcaaggca tttctcacca ccgaagctgc cactgccaag cgcctcgtcc
      421 gcgccggcca gaaaatccgc gaagcgcgcg ttccatttga aatcccggcg ggcgcggaat
      481 cgtcgcagcg tttggatgcc gttctgcaaa cgctttatct gcttttcaac gaaggttaca
      541 aggcatccag cggcagcaag cttgtgcggg aagaactttg caaggaagcg atacggctgg
      601 cgtcgctgtt ggctgaacat cccgtcggca atcgtccgca gacgcacgcg ctgctggcgt
      661 tgatgttctt gaacaccgcg cgattgcgcg gccgcatgga tgacgaaggc aatttgctgc
      721 gcttgaagga gcaggaccgc ggccgttggg accagacatt gattgcgcgc ggaattttcc
      781 atctggcgca gtcggcttcc ggcgaagaaa tttccgaata tcacctccaa gccggcatcg
      841 cggcagtcca ttgcgcggcg aaagattatg aatccaccga ctggccgcaa atcctttcgc
      901 tttacgaccg cttgattgaa tttgacgact cgccggtggt tgcgttgaac cgggccgtgg
      961 cgctggcgaa agtccacggc gcgcaagccg gccttgatgc cattgcggcg atccctgacc
     1021 gtgaaaaact ggaagaatat tatctgctct acgctgtgct cggcgaattc gcgacgcaac
     1081 tgaacgacca gcaaagcgcc gccgctcatt tccaaaaatc gcttcaactc gccgaactca
     1141 aatccgagaa ggcatttctc gaacacagac tgcgcatttg cgaggaacaa atcagccttt
     1201 aaaactaagg aattcagggt agagtaagcg aagtgggagg aggcggattg aaccgaccgt
     1261 agttgtcagt tccgcacttc cacttcgctt actctacccg agcgggattc atctgacgca
     1321 actttggaag gcgccggcaa gaatttcagc attgcgcgaa atcaaaaaat tctgctcgac
     1381 ccatcgcaga ccgttcgcgc cgagtttttt ccgaagcatg gaatcttctg ccaggcgttt
     1441 gacggcttgg gccaattgtt ccgcgtttgt cgcgtccacg accaggccgt tgatgccgtc
     1501 tgcgacggct tcaagattgc ccggcagatt gctggcgatc accgggagcg aatgcgcgaa
     1561 ggcttcggga atcacgttcg gcaggccgtc gcggtcgcct tcggcgtcca ccacgccggt
     1621 gtgccagaaa atatcggccc aataataagc ttcatccatc gcgtcgggcg gcgtcgcgcc
     1681 gcaaagtgtc acacaatctt gcaggcccgc gtgttgaatc gcctgttgca attctccgcg
     1741 caaggggccg tcgccaatga ttcgcgcgac aaacggcacg tcccacgatt tcaacaatgc
     1801 gcacgcggcg atctggtgga catggccctt tttcggcacc aatcgcccaa ccgacagtag
     1861 cgcgatggcc cggtaacacg tcgcgcgcga caccttgtcg ggcaatgatt caagtccgcg
     1921 cctcgcgagc acgattttga tttccgcgcc gcctcctcgt tcgcgcaaat atttttcggc
     1981 aacggcggtg gtggtgtgga cgaatcgggc ggagtgcaat ttgggcttta aaaaagcgtc
     2041 gccgctgtgc cggtaaatat catacgcgtg agcgccgaaa gtgaatggaa ttccgcaaag
     2101 ccggctgagt atcgccgcag ccgtcgcagg cccggtggcg cgaacgccgt gcaggacatc
     2161 cggcttgcgt tcacgaaatt tttttgcctg acagatggca aagatgaccg cccaaagtgt
     2221 tgcccaaaaa ttctcccgat ctttgaaacg a
//
LOCUS       DASZMM010000150         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2258791, whole genome shotgun sequence.
ACCESSION   DASZMM010000150 DASZMM010000000
VERSION     DASZMM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258791"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VG754_03795"
     CDS             <1..298
                     /locus_tag="VG754_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18360.1"
                     /translation="WTFNSADVYRSEEDFDWRKLAKHWGRVYGATSDSNGIAVVSNLP
                     AFKQHFAVWHTNFDLPIDPRDNDRHAATPLLPGQTNRVTVTLQKKGAKFLQSPP"
     gene            complement(342..693)
                     /gene="ssrA"
                     /locus_tag="VG754_03800"
     tmRNA           complement(342..693)
                     /gene="ssrA"
                     /locus_tag="VG754_03800"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1187..1543)
                     /locus_tag="VG754_03805"
     CDS             complement(1187..1543)
                     /locus_tag="VG754_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18361.1"
                     /translation="MEKDALITAGLAALTFAAFASWFLMARRPQQWAAWVERENDFWR
                     DKGLISARLTEKLKRWEKGKSLKILAGVTGFIGIVGLCLTLSVWVKVIFLRHQKLRGP
                     YNPALHLKLPPPGPKS"
     gene            complement(1606..3201)
                     /locus_tag="VG754_03810"
     CDS             complement(1606..3201)
                     /locus_tag="VG754_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF18362.1"
                     /translation="MSVLPIVDRELRVTARRKGIYWMRFYAALSMLLIWLVVLLNTNL
                     AARGQMGHYLLTSLGVLALAMSMLAGVFLTADSLSEEKREGTLGLLFLTDLKAYDVVL
                     GKMAAHSLHAFFGLLAMLPILGLTILMGGVTGKEFSRVALVFIATLFFSLSLGMAVSA
                     VCREARQAITTTFFLMLLFAGLMPALWWLQRMTINDSRLNFLLWPSPVGAYMAALDNA
                     YKYGKGAQFFWSSLGTIFCLGLIAILLANFVLPRSWHDKVQVKKAKRRWFKRASRETR
                     FTPFKYVLHYEPAFWLAVRDPSPERAGRRMVLLLMPAWFCALLISITTHWAHEGYIMA
                     LFVAYGLHLSVKVLLAVEASRRMNQDRRSGAWELLLVTPLSVKSILAGQRKALWLHFR
                     GVFLALFSVNMLMLMAALIFHKHLGMSSDDQWMFADMFAGGIAVLLMDFYALGWVGMW
                     GGLKAKQHHNAVVRTLGQVMLPPLVLVFLLFAIQPNFRSIAGPMIAMALWFGMGMALD
                     MASGMIARRRLLAEFRAAALLNR"
BASE COUNT          816 a         1000 c          749 g          687 t
ORIGIN      
        1 ctggacattt aattcggcgg atgtgtaccg gtcggaagaa gattttgact ggcgcaagct
       61 tgcgaaacat tggggccggg tctatggagc gacttcggac agcaatggta ttgcggtcgt
      121 atcgaacctg cccgccttca aacaacactt cgccgtctgg cacaccaact ttgaccttcc
      181 catagaccca agagataatg atcgccacgc ggccaccccg ttgctgccgg gccaaaccaa
      241 ccgcgtcacc gtgaccctgc aaaagaaagg cgcaaagttt ttgcaatcgc cgccttgaaa
      301 aaacaaacgc agacctgcaa acctgaaaaa actgttaaat atggtggagg cggcgggagt
      361 tgaacccgcg tcccagatta atcaaccagc cgcgactaca tgcttagtca gaagagagtt
      421 ttctgctgtg cgattacgct ctgacccgat tcgcaccgcc tagtccgcat gtaattttct
      481 cgaccatccg cgcgcggtct ccgcgtctgg tccaacctgc tgtggcgtcc gtccaccgta
      541 gcaggtgtcc agtggcggac gtcgtggcac ttaggccgcg agagctagtt cttgattagc
      601 ttttattttt ggtccgattt tttacgaggc caacgaacca tcctcggcat gccgcctctg
      661 gcgtattctt ctggtcgaaa ccagtacgcc cccgaaaacg acttccttat tatcggctgg
      721 gcgcagcctc gggtcaagac cccagatgct ggctaaactt tgcaaatcgc aattccccga
      781 gaagccccgg tcttgattcg cttgaggctg gaaatcccaa atagctgcaa atcagcgttt
      841 tttgaatttg tattcgaaaa tccaaaaaac catcaacgcc agcatggccg caaagccgag
      901 gccaaccgta atgttcatca aaatattcat agttctgtca cttgttcgat tcatcgaatc
      961 ttcttcagta gaatcgtagg aaggaccgcc ttcgctggga atatggtatt ttgcgaaatg
     1021 gctccttcac cttgtcctac gcgataataa gctggggtga ttaccccatt tggcgagtgg
     1081 ggtatttcag gcagactctc accacgttga ttcgggtggc tgaatgtctt ccgcggggtc
     1141 cctcaccctc acctccttgg cttcggctgc ccgaatcgct tccttttcag gactttgggc
     1201 cgggcggtgg caatttcagg tgcaaggcag gattataagg cccacgaagc ttttgatgcc
     1261 gcaagaaaat cacctttacc cataccgaga gcgtcaggca aagtccaaca atccctataa
     1321 atcccgtaac gccagccaag attttcaacg actttccttt ctcccaacgc tttaatttct
     1381 ccgtcagcct ggccgaaatc agccctttgt cgcgccagaa atcgttttcg cgctccaccc
     1441 acgcggccca ttgttgcgga cgccgcgcca tgaggaacca actggcgaac gccgcaaacg
     1501 tcagcgccgc caatccagcc gtgatcaatg catctttttc cacgcgggct ccatttgata
     1561 tttcccggcc atcattcgca atctgcaatt ccaatcaatt tcaattcaac gattcaacaa
     1621 cgccgccgcg cgaaattccg ccagcaaccg ccttcgcgct atcatgccgg atgccatgtc
     1681 cagcgccatt cccattccga accaaagcgc catcgcaatc atcggtcccg cgatgcttct
     1741 gaagttcggt tgtatcgcaa acaacagaaa aacaaggacc agaggaggca gcatgacctg
     1801 ccccaaagtc ctgaccactg cattgtgatg ctgttttgct ttcaatccgc cccacattcc
     1861 cacccagccc aacgcataaa agtccatcag caacactgcg attccacccg caaacatgtc
     1921 ggcgaacatc cattgatcat cggaactcat gcccaggtgc ttgtgaaaaa tcagcgctgc
     1981 catcagcatc aacatgttca ccgaaaaaag cgccagaaac actccgcgga aatgcagcca
     2041 caaagctttt ctttgtccgg ccaggatgga cttcaccgac aagggcgtca cgagcagcaa
     2101 ttcccacgcg ccactgcgcc ggtcctggtt catccggcgg cttgcttcca ccgccagcaa
     2161 tactttgact gaaagatgca atccgtatgc gacgaacaag gccatgatat aaccttcatg
     2221 cgcccaatgt gtcgtgatgg agatcagcaa agcgcaaaac caggcaggca tcagcaatag
     2281 caccattcgc cggcccgccc tttccggcga agggtcccgc accgccagcc aaaaggccgg
     2341 ttcataatgc aaaacatatt tgaacggcgt gaatctggtc tctcgcgaag ctcgtttaaa
     2401 ccaccgtctc ttcgcttttt tcacctgcac cttgtcgtgc cacgaacgcg gcaatacaaa
     2461 attggcaagg aggatcgcaa tcaatcccaa gcaaaaaatc gtgccgagcg atgaccagaa
     2521 aaactgcgcg cccttgccgt atttgtatgc attatcaagg gctgccatgt aagcgcccac
     2581 cgggctgggc cacagcagaa aattcagcct gctgtcgttg attgtcatcc gttgaagcca
     2641 ccacagggcc ggcatcaaac ccgcaaacaa cagcatcaga aaaaacgtgg tcgtaatcgc
     2701 ctgcctcgcc tcacggcaaa ccgccgacac cgccattccc aaactcaacg aaaaaaagag
     2761 cgtcgcgata aacacgagcg caacgcgcga aaattccttc ccggtcactc cgcccatcaa
     2821 tatcgtcaag ccgaggattg gcagcatcgc cagcaaacca aaaaatgcgt gcaacgaatg
     2881 cgcggccatt ttgcccagca ccacgtcata agccttcaag tccgtcagga aaagcaggcc
     2941 caaggttcct tccctctttt cttccgacaa cgaatccgcc gtgagaaaaa ccccggccaa
     3001 catgctcatc gccagcgcca gcacgccaag gctcgtcaac aggtaatggc ccatctggcc
     3061 ccgcgccgcc aaattggtgt tgagcaacac caccagccag atcagcaaca tgctcagtgc
     3121 cgcgtaaaag cgcatccaat agatgccctt gcggcgcgcc gtcacgcgca gttcccggtc
     3181 aacgatgggc agcacactca tcggcaaaca ctttgcggat tggacattgt tttacaagag
     3241 tcgaatggac gc
//
LOCUS       DASZMM010000151         6115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1706325, whole genome shotgun sequence.
ACCESSION   DASZMM010000151 DASZMM010000000
VERSION     DASZMM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706325"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VG754_03815"
     CDS             <1..1444
                     /locus_tag="VG754_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWF18363.1"
                     /translation="QWGGATAAWGGRCIPFDAIDFEKRSWVPHSGWPITKAQLDPFYA
                     RALALCEAGEYKFFTEETFPEKQREMIAGFDGPDVVSNRMERWGPPTHFGKRYARDLE
                     SASNVTVLFHANCQRIQLHSEGQRVEQITVASFERNEFFVRARFFVLACGGLENTRLL
                     LASNDVMKTGIGNHSDKLGRYYMSHLFGALAMAKLRDNGGSFIFDLERDREQVYCRRR
                     FWITSGAQKKNEILNAIAFFFRPPLGRAVHRNALFSSAYLAKFFLTTFKRNSPSKAAA
                     ILRENRAALLAHLKIVAADAPHLVPEVAAIVKNRWFARRRLPFVLPRKSHNHYCLYYQ
                     SEHAPNPESRVMLHSEKDFFGMPRLLVKIKFSEMDVESVLRAHRLIRDQFAATKTGEL
                     VYDEAALREDVRHEIQEFNSSAHQLGTTRMSASPTDGVVDADCRVHGAQNLFVAGSSV
                     FPTSGHANPTLTIVALAVRLADYLKASLRE"
     gene            complement(1450..3045)
                     /locus_tag="VG754_03820"
     CDS             complement(1450..3045)
                     /locus_tag="VG754_03820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWF18364.1"
                     /translation="MNQEARDASNLVPGRWERQSNWAVHLVQLFFLGVMLFQLDAPFL
                     SAHNERQNQTFDMARHVFRDGWRSVITPKVSYCIPGFEKEPVTAVLQEVPFHGLLGWP
                     LVLATGHERAAVRIVSTVFALLSIQLLYWILRFWLPPVVSALGAAIWATAPLVLHFGQ
                     VPMPDILCATGILAAFWFALRGKLPASSGCFGFAILAKMSVIVFGLPVLVALLVARNC
                     NSLRDFLRLSLLWGWFPLLSLATWEIVLYSFAPSTPMSVVQIWQEGKPWSLFAHLVIY
                     KFLLGCIFPFGIGVLGMAGLLFAVRAEKSPMDARIKWAIIVSNLIFLAFVIRKVAEPQ
                     YVLQMLPWFVIGASFGLDYFLKKLCAGIGWRIGFGMVLILHILVALFFTMDLKTSRVH
                     NLPDIERAGELLPPDARVVVIYRFYGAGPAVWLDRNVVPMGVPDRLEESFTRLQAEGF
                     SHVLILDIESLHNRRSAKDPQALLARIIGAVGRAPSIENANMPAFTDPAGPFRQFCDR
                     NFSQMFAAPHVVLYSIAPWHAKN"
     gene            complement(3052..4017)
                     /locus_tag="VG754_03825"
     CDS             complement(3052..4017)
                     /locus_tag="VG754_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18365.1"
                     /translation="MSIHRTGKMALKSGFAAFTSALLVLFALNASASSGEPDVTSSSP
                     LQLYNDGTKRLREGKLREAEAALQGAVTSQETRVQGPALYNLGETRFKDGAQDLKKAP
                     NPKVMSDVSKQASDNAGGAIESADKALAGEDLQAMVEAYYRGRGARKELKAAIAAVKA
                     AMESYGAVLAKWQRASGDFKSDYELVPKDTDARTNADLVDQHIAKLVDTHQMMMQMMN
                     AMGKQRSELGKKMQELKKKMPGDQGQQFKGGDEDDDEDEDGKKPKEPKEGMEEPGPKN
                     GKEMVLSYEEAARLLDMLKLDANRKLPLGGTNAAAAPKDRKGRDW"
     gene            complement(4014..5048)
                     /locus_tag="VG754_03830"
     CDS             complement(4014..5048)
                     /locus_tag="VG754_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF18366.1"
                     /translation="MDFRHPHFAEPGWLWLALLAPVALVLLQRYAAWARKRQLTLFAA
                     PELLADLLRSHSPARRVVKNIFLVLAIAAMGIALARPQWGETTEISQTLGEDILFVLD
                     CSKSMLATDVAPSRLTRAKYAILDFVQKHGRGRVGLVAFSGQAFLQCPLTFDYDAFRE
                     ALLAVDEKTIPVPGTDIGRALDEGFRAMEKNERHKVMVLVTDGEDLENSGVNTAKTLA
                     EKGIVVHAIGVGTTAGSPIQVVNDRGLPETLRDRDGAVVVSHLDETTLTAIANATHGS
                     YEPLGSLGEGLNRVRKLVEDSSNTAQSSKMRKLGVDRFYIPVGLLTMLLAVESLIGTR
                     RKLREHGTGE"
     gene            complement(5057..>6115)
                     /locus_tag="VG754_03835"
     CDS             complement(5057..>6115)
                     /locus_tag="VG754_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF18367.1"
                     /translation="SSQDLMTSHFQFQFPWLLALLALLPVYAFLRGRIGNMSALRFSS
                     ADIARAAGGAARAAAGRLLLFMRLLVLALCIVALAGPRFANDRTETQASGVDIMLVLD
                     LSWSMESLDMGGTTEETTRFGIASSVLEDFIGKRPNDRIGLIAFSGVPYLASPLTLNH
                     EWLAQNLQRLHVGTIRELGTAIGDATAAAAKRLEMLKGSKSRIIILLTDGDNNQGEID
                     PVPAAQVAAAIGAKIYTIGIDIEEPCRLPAFDVNTGKLRRGEEGQVIYSGMILQPANY
                     SVLGKMANLARGRFFRAKNRSELQSIYNEIDRLEKTEVKLRRYTTFTPLFQWPLLAAF
                     ALLGLELILANTRYRRLP"
BASE COUNT         1362 a         1874 c         1578 g         1301 t
ORIGIN      
        1 tcaatggggc ggcgcgaccg ccgcatgggg cgggcgttgc attccgttcg atgcaattga
       61 tttcgaaaaa cgctcatggg ttccgcacag cggctggcca atcacgaaag cgcagttgga
      121 cccgttttat gcgcgcgcgc tcgcgctttg tgaagcgggt gaatataaat ttttcaccga
      181 ggaaacattc ccagaaaagc aacgcgagat gatcgcgggg tttgatggcc ccgacgtggt
      241 ttccaaccgc atggaacgct ggggtccgcc aacgcacttc ggaaaacgct atgcgcgcga
      301 cctggaatcc gcgtccaacg tgaccgtcct gtttcacgcc aattgtcagc gcatccaact
      361 tcattccgaa ggtcagcgag ttgaacagat caccgtcgcg tcgtttgaac ggaatgaatt
      421 ttttgttcgc gcgcggtttt tcgtcctggc gtgcggcggc ttggaaaaca cccgtctgct
      481 gctggcgtcc aacgacgtga tgaaaaccgg catcggcaat cattccgaca agctgggacg
      541 ttattacatg tcgcatctgt tcggagcatt ggccatggcc aaattgcgcg acaatggcgg
      601 gagtttcatt tttgatctgg aacgcgaccg cgaacaggtc tattgccgtc gccgtttctg
      661 gatcacctca ggcgcgcaga aaaaaaatga aatactcaat gccatcgcct tctttttccg
      721 cccgcctctt ggccgcgccg tgcatcgcaa cgccctgttt tcgagcgcct atcttgcaaa
      781 atttttcctg accactttca agcgcaacag tccctcgaag gccgctgcga tcttgcggga
      841 aaaccgcgcc gcgcttctcg cccatctgaa aattgtcgcc gccgatgcgc cgcatcttgt
      901 gccggaagtc gccgccatcg tcaaaaatcg ctggttcgcc cgccgccgtt tgccgttcgt
      961 ccttccgcgc aaaagccata accattattg cctctattat cagtccgagc acgcgcccaa
     1021 tcccgaaagc cgcgtgatgt tgcactcgga aaaggatttt tttgggatgc cgcgcctgct
     1081 ggtgaaaatc aaattttccg aaatggatgt cgaatcggta ctgcgggcac accggttgat
     1141 tcgcgaccaa ttcgccgcca ccaaaacggg cgagttggtt tatgatgagg cggcattgcg
     1201 tgaagacgtg cggcatgaaa tccaggagtt caattcctcc gcgcatcaac tcggcaccac
     1261 ccgcatgtcc gcttctccaa ccgacggcgt ggtggatgcg gactgccggg tgcacggcgc
     1321 gcaaaatctt ttcgtggcgg gcagttcggt gtttcccacc agcggccatg cgaacccgac
     1381 cctcaccatc gttgcgctgg ctgtccgcct ggccgattac ttgaaagcct ctttgcgaga
     1441 atgaaactgt taatttttcg cgtgccacgg cgcaatggaa tagagaacca catggggcgc
     1501 cgcaaacatt tgtgaaaaat tccgatcgca aaactggcgg aaaggcccgg ccggatcggt
     1561 aaatgccggc atgtttgcat tttcgattga aggcgcgcgg ccaactgctc caataatcct
     1621 tgccaacagg gcttgagggt cctttgcact tctcctgttg tgcaggcttt caatgtccag
     1681 aatgagaacg tggctgaatc cttccgcttg cagcctggta aatgattctt ccagcctgtc
     1741 cggcacgccc atcggcacca cgttccggtc cagccatacg gcaggcccgg cgccataaaa
     1801 acgataaatc acgacgacgc gcgcatccgg cggaagcaat tcgccggcgc gctcaatgtc
     1861 aggcaggttg tggacccgcg aagttttcag gtccatcgtg aaaaagagcg cgacaagaat
     1921 gtgcaatatc aggaccatgc caaagccgat tcgccagccg atgccagcgc aaagtttctt
     1981 taaaaaataa tcaagcccga acgacgctcc gatcacgaac cagggaagca tttgcaggac
     2041 gtattgcggc tccgcgactt tgcggatcac aaacgcgaga aagataagat tcgaaacaat
     2101 gatggcccat ttgatccgtg catccatcgg ggatttttcc gcgcgaacgg caaacaacag
     2161 gcccgccatg cccaacactc cgattccaaa tggaaaaatg cagccgagga gaaatttgta
     2221 aatcaccagg tgcgcaaaaa gactccacgg ctttccttcc tgccagattt ggaccacgct
     2281 catgggagtg gatggcgcga agctgtaaag cacaatctcc caggtcgcga gacttaacaa
     2341 cggaaaccat ccccacaaca gcgacaaacg cagaaaatcc cggagcgagt tgcaatttcg
     2401 cgcaacaagc aacgcaacca aaaccggcaa tccgaaaaca atgacgctca ttttcgcgag
     2461 tatcgcgaat ccaaagcaac cgctggacgc cggtaatttt ccgcgcaacg caaaccaaaa
     2521 cgccgccaga attccggtcg cgcagagaat gtccggcatc ggcacctggc caaaatgaag
     2581 gaccaacggc gctgtcgccc aaatcgccgc gccgagcgcc gaaacgaccg gtggcagcca
     2641 aaagcggaga atccaataca atagttggat tgaaagcagc gcgaagacgg tggaaacaat
     2701 ccgcaccgcc gcgcgttcat gaccggtcgc cagcaccaac ggccatccga gcaatccgtg
     2761 aaagggaact tcctgtagca cggctgtgac tggttccttt tcaaaaccgg ggatgcaata
     2821 ggatactttt ggagttatca cagagcgcca gccatcacga aaaacatggc gcgccatgtc
     2881 gaaggtctgg ttctgccgct cgttatgggc ggaaagaaac ggagcgtcca attggaacaa
     2941 catcacgccc aaaaaaaata gctgaaccag atgcaccgcc caattcgact gcctttccca
     3001 acgtcccgga actaaattgg aggcatcacg tgcttcttgg ttcatcgcat ttcaccaatc
     3061 gcggcctttg cggtccttgg gagcggctgc ggcattggtt ccacccaatg gcagtttgcg
     3121 attggcgtcg agtttcaaca tgtcaagcaa ccgcgcggct tcctcgtaac tcaagaccat
     3181 ttccttgccg ttttttggtc ccggctcttc catgccttcc ttcggctcct tcggtttttt
     3241 gccgtcctcg tcctcatcat catcttcatc gccgcccttg aattgctggc cctggtcgcc
     3301 gggcattttc tttttcaatt cctgcatctt tttgcccagt tcgctgcgct gtttgcccat
     3361 ggcgttcatc atctgcatca tcatctggtg tgtgtccacg agcttggcga tgtgctggtc
     3421 caccaaatcg gcgttcgtcc tggcatcagt gtctttcggg accagttcgt aatcgctctt
     3481 gaaatccccg gaagcgcgct gccatttcgc cagcactgcg ccgtaacttt ccatcgccgc
     3541 cttcaccgcc gctatggcag cttttaattc cttgcgcgcg ccgcggcccc gataatacgc
     3601 ctccaccatc gcctgcaaat cctcgcccgc caacgccttg tccgctgact cgattgcgcc
     3661 gcccgcattg tccgacgcct gtttggaaac atcgctcatc accttgggat ttggcgcttt
     3721 cttcaaatct tgcgctccgt ccttgaaacg cgtctcgccc aaattgtaga gcgccggccc
     3781 ttgcacccgc gtttcctggc tggtgaccgc gccttgcaga gcagcttcgg cttcccgcaa
     3841 tttgccttcg cgcaatcttt tggtgccgtc gttgtaaagc tggagcggtg aagaggacgt
     3901 gacatcaggt tcgcctgacg acgcggaagc attgagcgca aataaaacaa gcagggccga
     3961 agtgaaggcc gcgaacccgc ttttcaacgc cattttgcct gttcgatgaa tgctcattcg
     4021 ccagtaccat gttcgcgcaa ttttcgccgc gtgccaatca aagattcgac cgccagcaac
     4081 atcgtcaaca agccgaccgg aatataaaat cggtccacgc ccaacttgcg catcttggaa
     4141 gactgggctg tgtttgagga gtcttccaca agtttccgca cgcgattgag cccttcgccc
     4201 agcgagccga gcggttcgta actgccgtgc gttgcgttcg cgatggccgt caaagtggtt
     4261 tcatccaaat gactcacgac caccgcgcca tcgcggtcgc gcaatgtctc cggcaacccc
     4321 cgatcattga ccacttggat tggactgccc gccgtcgtgc ccacaccgat ggcgtggacg
     4381 acaattccct tttccgccaa agttttcgcg gtgttcacgc cggaattttc caaatcctcg
     4441 ccgtccgtca ccagcaccat caccttgtgc cgctcatttt tttccatcgc gcggaaacct
     4501 tcatcaagcg cgcggccgat gtccgtgccc ggcaccggaa tcgttttttc atccaccgca
     4561 agcaacgctt cgcgaaatgc atcgtaatca aacgtcagcg ggcattgcaa aaacgcctgt
     4621 cccgaaaacg ccaccaaccc cacgcgaccg cgcccatgct tttgcacaaa atccaggatg
     4681 gcgtatttcg cgcgcgtgag acggctcggc gcgacatccg tcgccaacat gcttttggaa
     4741 caatccagca caaaaaggat gtcctcgccc agcgtttgcg aaatctctgt cgtctcgccc
     4801 cattgcggcc gggccagcgc gatgcccata gcagcgatcg ccagcactaa aaaaatgttt
     4861 ttcaccacgc ggcgcgccgg actgtgcgaa cgcaacagat cggcaagcaa ttccggcgcg
     4921 gcgaacaaag tcaattgccg tttcctcgcc catgcggcgt aacgctgcaa cagcaccagc
     4981 gcaaccggcg ccagcagcgc cagccacaac caacccggtt ccgcaaaatg tggatgtcga
     5041 aagtccattc tttaattcaa ggcaatcgcc gatagcgcgt gttcgccaaa atcaattcca
     5101 gccccagcag cgcaaacgcc gcaagcaacg gccattgaaa caacggcgta aatgttgtgt
     5161 aacggcgcaa cttcacttct gttttttcca gccggtcaat ctcgttgtaa atgctttgca
     5221 attcgctccg gtttttcgct cggaaaaaac ggccccgcgc aagattcgcc atctttccga
     5281 gaaccgaata atttgccggc tgcaaaatca tgccggaata aatcacctgg ccttcctctc
     5341 cgcggcgcaa tttgccggtg ttgacgtcaa atgcgggcag ccggcaaggc tcttcgatgt
     5401 cgatgccgat ggtgtaaatc ttcgcgccga tcgcggcggc gacttgcgcg gcgggaacgg
     5461 ggtcgatctc gccctggttg ttgtcaccgt cggtcagcag gatgatgatg cggctcttgc
     5521 tgcctttgag catttccagg cgcttggccg cggcagcggt cgcatcgcca atcgccgtgc
     5581 ccaattcgcg gatggtgccg acatgcagac gctgcaaatt ttgcgcgagc cattcgtgat
     5641 tgagcgtcaa tggacttgcc agatacggaa cgccggaaaa agcgatcagc ccgatgcggt
     5701 cgttcgggcg cttgcctatg aaatcttcca aaacggaact ggcaatgccg aagcgcgtgg
     5761 tttcctctgt cgtgccgccc atgtcgagcg attccatgga ccacgacaaa tcgagcacca
     5821 gcatgatgtc cacgccgctc gcctgcgttt ccgtgcggtc atttgcgaaa cgcggcccgg
     5881 ccagcgccac gatgcagagc gccagcacaa gcaatcgcat gaacaacaac aaccgtcccg
     5941 ccgctgcccg cgccgcgccg cctgccgcgc gcgcaatgtc cgcgctcgaa aatcgcaatg
     6001 cggacatatt cccgatgcgt ccgcgcaaaa atgcatatac cggcagcaac gccagcagcg
     6061 ccagcagcca gggaaattga aattggaaat gcgacgtcat caggtcttga ctgga
//
LOCUS       DASZMM010000152         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3903663, whole genome shotgun sequence.
ACCESSION   DASZMM010000152 DASZMM010000000
VERSION     DASZMM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3903663"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2349
                     /locus_tag="VG754_03840"
     CDS             <1..2349
                     /locus_tag="VG754_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF18368.1"
                     /translation="QGGQFNEYGIVTHEGVARVFSDGTLDTNFNNTTNFNTPLVRCLA
                     LQSDGKAIVGGNFFTWATSGRTNLVRLYGNNYPAEIITQPKSINVAVGTNVTFFTDVS
                     NPTQVDYQWRYDGQDILGATDNEYSIFNAQLADAGTYSVFVNSGVGGTTSSNAVLQVG
                     IPPAITQQPTPTSLAVTQGQTATFTAAASGTPLNYYWLLNGKSLAGTNATLTVTNVLP
                     NQAGNYTLVASNFLGTATSAPAALSVLYPNNIVNGPSSQSVAVGGTALFTVSASGNPL
                     NYQWLKSGSPIPGATGSSFQITNVMFSDAASYSVTVSNQFNSQTSSVATLSVGYPPSI
                     TLQPTNVTANLGDTVSFTSAASGTGPLSYQWLFNNNPIANQTASNLTLTNVQIGNIGN
                     YALSVSSPFGSAISSNAFLNLNGYPSNLYFGLIAYYPFSGNANDATGNGNNGTVSGAA
                     LTQDRFGNPGMAYQFDGVSNYIYCSIVNIPAGSAPRALSLWAQSDATNTAGANPAWWG
                     SQQSLQGFGIEAYGTPYLWAGQLDGGVDVVYSDAVVDTNWHQLVLTYDGATLAIAVDG
                     VQQNNLGVTANTSISGLYIGSGLAANYFPGAVDEVRIYNRALSTNDVAQLYQLESGLP
                     LIQQQPQSLSLVVGQTANFNVQVASIGSLSYQWYYSNAPLANATNALLTIPSVQMTNA
                     GAYFVVVSNTLGSATSGVANLTVGTPPQMALVHFGPNNLPMIQMSGTSGFNYMVQTTT
                     NLTPPIVWQTIATIPADTNGNWTFIDTNPPSLGSRFYRVAAP"
     gene            complement(2442..>2657)
                     /locus_tag="VG754_03845"
     CDS             complement(2442..>2657)
                     /locus_tag="VG754_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18369.1"
                     /translation="APGVHRSNTTPEDISRTAVPHVTYLLHRDPPPKKLAELRRRMRE
                     RVQERVEKIRDVRKKIRLPIAGKSQAV"
BASE COUNT          603 a          782 c          684 g          588 t
ORIGIN      
        1 caaggcggac aattcaatga gtacggcatt gtgactcacg aaggggtggc gcgggttttt
       61 tccgacggca cgctcgacac aaatttcaac aacacgacaa acttcaacac gccactggtg
      121 cgttgccttg ccctccaaag cgatggaaaa gccatcgttg gaggcaactt ttttacctgg
      181 gcaacgtctg gaaggaccaa tctggttcgg ctttatggca acaattatcc ggctgaaatc
      241 atcacgcagc cgaaaagcat caatgtcgcg gtcggcacaa atgtcacttt tttcacggac
      301 gtgagcaacc ccacacaggt ggattatcaa tggcgctacg acggacagga catcctcggc
      361 gcgaccgaca acgaatattc catcttcaat gctcaactcg ctgacgccgg cacttacagt
      421 gttttcgtca acagcggcgt cggcggaaca accagttcca atgccgtgtt gcaagtcggc
      481 atcccgcccg cgatcacaca acaaccgacg ccgacaagcc ttgccgtcac tcagggacaa
      541 accgcaacat tcacagcggc ggcaagcggc acaccgttga attattactg gttgttgaac
      601 ggaaagagcc tggccggaac aaacgccacc ttgaccgtca caaatgtgct gcccaaccaa
      661 gccggcaatt ataccctggt ggcctccaat tttctcggca ccgcaaccag cgcgcctgcc
      721 gctttatccg tgctttatcc caacaacata gtcaatggcc cttccagcca atcagtcgca
      781 gttggcggca cagcgctctt taccgtctcc gcctccggaa atccgttgaa ttatcaatgg
      841 ctcaaaagtg gcagtccaat cccgggcgcc accggttcga gtttccagat cacgaacgtc
      901 atgttcagcg atgcagccag ctacagcgtg acggtttcaa accagttcaa cagccagacc
      961 agttccgtcg ccacactctc cgtcgggtac ccgccgagca tcaccttgca gccgacgaac
     1021 gtgacggcca atttgggcga cacggtttca ttcaccagcg ccgcttcggg aacaggacct
     1081 ttatcttatc aatggctgtt caataacaac ccgatcgcaa atcagaccgc cagcaatctg
     1141 acactcacca acgtgcagat cgggaacatc ggcaattacg cgctgtccgt atcaagccct
     1201 tttggaagcg ctatcagttc caatgcgttc ctcaatctga atggttatcc ctcgaatctc
     1261 tatttcggtt tgatcgcgta ttatccattc agtggaaatg ccaatgatgc gactggaaat
     1321 gggaacaacg gcacggtcag cggagcggcc ttgacacagg accgttttgg caatccgggg
     1381 atggcatatc aattcgacgg cgtgagcaat tatatctatt gcagcatcgt gaacatcccg
     1441 gccggttctg cgccccgggc actttcgttg tgggcccagt cggacgcgac aaatacagcc
     1501 ggcgcaaacc cggcatggtg gggttcgcaa cagtccctgc agggtttcgg aattgaagcc
     1561 tacggcacgc cttacctttg ggccggccag cttgatggtg gcgtggacgt tgtctattcc
     1621 gatgcggttg tagatacaaa ctggcatcaa cttgtgctga cgtatgatgg cgctacgctc
     1681 gccattgcgg tggatggcgt gcaacaaaac aacttgggcg tgaccgctaa cacctcaatc
     1741 agtggtctgt atatcggttc gggactcgca gccaattatt ttcccggcgc tgtggatgaa
     1801 gtccgcatct acaatcgcgc cttgtccacg aatgatgtcg cgcaacttta tcagttggaa
     1861 agtggcctgc cgctcatcca gcaacagcca caatcgttgt cgctggttgt ggggcaaacg
     1921 gccaatttca atgttcaggt tgcgagcatc gggagcctca gttatcaatg gtattattcc
     1981 aatgcgcccc tggcgaacgc cacaaatgcg ctgctgacca tcccttccgt tcagatgacg
     2041 aatgccggcg catatttcgt ggtggtgtcc aacaccctcg gttctgcaac aagcggcgtg
     2101 gcaaacctca cggtgggcac tcctccgcaa atggccctgg ttcattttgg tccaaacaac
     2161 ctgcctatga tccaaatgtc cggaacatcg ggtttcaact acatggtgca aaccaccacc
     2221 aatctgacgc ctcccatcgt ttggcaaacc atcgccacca tcccggcgga caccaatggg
     2281 aattggactt tcatagacac aaatccgccg tcattggggt cgcggtttta ccgcgtcgcg
     2341 gcgccgtagc cgatgaaaaa ggggagcgca cgcgtctcgc gtgcagtgtt cggcgtcccg
     2401 ccgaacgctt cgtaatactg attatgtcct tgtttaaact gttaaacagc ctggcttttt
     2461 ccagcgatgg gcaaccggat ttttttgcgg acgtcgcgga ttttttcgac acgttcctgg
     2521 acgcgctcgc gcatccggcg gcgcaactcg gcaagctttt tcggcggcgg atcgcgatgc
     2581 agcagatagg tcacgtgcgg cacggcggtg cgcgaaatgt cctccggagt ggtgttgctg
     2641 cgatgcactc ccggcgc
//
LOCUS       DASZMM010000153         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4193142, whole genome shotgun sequence.
ACCESSION   DASZMM010000153 DASZMM010000000
VERSION     DASZMM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4193142"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VG754_03850"
     CDS             complement(<1..1118)
                     /locus_tag="VG754_03850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF18370.1"
                     /translation="MTIYDAIIIGGGPGGSASASYLARAGKRVLVLEKEIFPRFHIGE
                     SLLPYNQQLFRELGLTKTLQEAGFIRKFGAQFHLGNGAKGTKFVFRQGVYTREPEAVQ
                     VERATFDHLLLKHARASGAEVREGWTVSKFTNEPGGIAIEARNGAGPAENFRAKFLID
                     ASGRGNVTGNQEGLRVIHPRLKKLAVFSHFNGVKVDEGDRGGDTVIIRLHNKWFWLIP
                     LTKEKVSVGCVMDQEEFARAKLPPAEIFQNIWQSSAAMRERMQAAKLIGAMHTTSDFS
                     YHNRRLVGERLLRVGDAAGFMDPIFSAGVFLAMFSGKLAAEAVIKSLDASDDGRERLM
                     KYEKRVRAAMQFYWEMVENFYTTPFMEIFLEPREKFNLP"
     gene            complement(1158..1436)
                     /locus_tag="VG754_03855"
     CDS             complement(1158..1436)
                     /locus_tag="VG754_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWF18371.1"
                     /translation="MEDSIELKQRIKEIMVENLMLQVSAAEIKDDQPLFGPGSLGLDS
                     VDALQLVVALDKNFGLKIPSPASAKEILHSVNSIAAAVQAKTAQAGAA"
     gene            complement(1442..2176)
                     /gene="fabG"
                     /locus_tag="VG754_03860"
     CDS             complement(1442..2176)
                     /gene="fabG"
                     /locus_tag="VG754_03860"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWF18372.1"
                     /translation="MGSTRIILITGANGGMGQALARSFLAESPENFVWLGIHNRREQA
                     DALATEFAGRCGCIELDVTKPDAWQATVAKIVSQHQRLDVLVNNAGFHQDGLLANLPA
                     SAWQQVIAANLDSVFHGCQAVLPTMISQRGGRIVNISSLSALLAPAGQTNYAAAKAGV
                     VALTQSLAKEVARIGITVNAICPGYIETEALNGMDAVERKAITARIPMRRMGAPAEVA
                     AAVRFLACPEAGYITGSVLKVDGGIF"
BASE COUNT          466 a          684 c          612 g          496 t
ORIGIN      
        1 gggagattga atttttcgcg cggttccaaa aaaatctcca tgaacggggt tgtgtaaaaa
       61 ttctccacca tctcccaata gaattgcatc gccgcgcgca cgcgtttttc atatttcatc
      121 agccgttcgc ggccatcgtc gctggcatcc aaagatttga tcacggcctc ggcggcgagt
      181 ttgccggaaa acatcgcgag aaaaacaccg gcggaaaaaa tcgggtccat gaaaccggcc
      241 gcgtcgccca ctcgcaacaa acgctcgccc acgagccggc gattgtgata cgaaaaatcg
      301 ctcgtggtat gcatggcgcc gatcaatttt gcagcctgca tccgttcccg catcgcggcg
      361 ctcgattgcc agatgttttg aaaaatctcc gccggcggca atttcgcgcg cgcaaattct
      421 tcctggtcca tcacgcagcc gacgctcact ttttccttcg tgagcggaat cagccagaac
      481 catttgttgt gcaaacggat gatcaccgtg tcgccgcctc ggtcgccttc atccaccttc
      541 accccgttga aatggctgaa cacagccagc tttttcaagc gcggatggat cacgcgcaaa
      601 ccttcctggt tgccggtgac attgccgcgt ccgctcgcat caatgagaaa tttggcgcgg
      661 aaattttcgg caggacctgc gccgttgcgc gcctcgattg cgatgccgcc gggttcattg
      721 gtgaatttgg agactgtcca gccttcgcgc acttccgcgc ccgaagctcg cgcatgtttc
      781 aacaaaagat gatcaaacgt cgcacgctcg acctgcaccg cttcaggttc gcgcgtgtaa
      841 actccttgtc gaaaaacgaa cttcgttccc ttcgcgccgt tgccgagatg gaattgcgcg
      901 ccgaatttgc ggatgaaccc ggcttcttgc aaagtcttgg tcaggcccaa ttcgcggaac
      961 aattgctgat tataggggag cagcgattcg ccgatgtgaa agcgcgggaa aatctccttc
     1021 tccaggacaa ggacgcgctt gcctgcgcgg gcaagatagg aagccgaggc gctgccgccc
     1081 ggtccgccgc cgatgatgat ggcatcgtaa attgtcatgg ttgccagccc tcatgatgcc
     1141 ccttcatcaa cgcggattca agctgcgcct gcttgcgcgg ttttggcctg gacggctgcg
     1201 gcgatgctgt tgacgctgtg caaaatttcc ttcgcgctcg cgggcgaggg gattttaagc
     1261 ccgaaatttt tgtccagcgc gacgacgagt tgcagcgcat cgacggaatc cagccccaaa
     1321 ctgccggggc cgaagagcgg ttggtcgtct ttgatttcgg cggcgctgac ctgcaacatc
     1381 aggttttcca ccatgatttc tttgatccgt tgtttgagtt cgattgagtc ttccatgatg
     1441 attaaaaaat cccgccgtcc actttcaaca ccgagcctgt gatgtaaccc gcctccggac
     1501 aagccagaaa acggaccgcc gccgcgactt cagcgggcgc gcccatgcgg cgcatcggaa
     1561 tgcgcgccgt gatcgccttg cgttcgaccg cgtccatccc gttcaacgct tcggtctcga
     1621 tgtagccggg gcaaatcgcg ttcacggtga tgccgatgcg cgcgacttcc tttgcgagcg
     1681 attgcgtcaa cgccacgacc ccggctttgg ctgcggcgta attggtctgg cctgcgggcg
     1741 cgagcagcgc gctcaacgac gaaatgttca cgatgcgtcc gccgcgctgc gaaatcatcg
     1801 tcggcaacac cgcctggcaa ccgtgaaaaa ccgaatcgag gttcgccgcg atgacttgtt
     1861 gccacgcgct cgcgggcaga ttggcgagca aaccgtcctg atgaaatccg gcattgttca
     1921 ccagcacatc gagccgttga tgctgtgaaa caattttggc gaccgtggcc tgccacgcgt
     1981 ccggtttggt gacatccaat tcaatgcagc cgcaacgccc ggcaaattcc gttgccaaag
     2041 cgtccgcctg ctcgcggcga ttgtgaatgc cgagccagac aaaattttcc ggcgattcgg
     2101 ccagaaaact tcgcgcaagc gcctggccca ttccgccatt cgcgccagtg attaaaatga
     2161 tccgggttga tcccatcgat taaattgcgg caaaatgtgc cccaattgcc tgctgattgc
     2221 aaccaaccac gctcacgctc gcggcagcgc aatgtttt
//
LOCUS       DASZMM010000154         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2537152, whole genome shotgun sequence.
ACCESSION   DASZMM010000154 DASZMM010000000
VERSION     DASZMM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537152"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..465
                     /locus_tag="VG754_03865"
     CDS             118..465
                     /locus_tag="VG754_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18373.1"
                     /translation="MKSGLTLGKCAQHFKLLWLRKLIGKPRVPYNRGLRIRGSSHPEF
                     YDADLSLPAAESNAFRFQSEFVDSADNTVKVGVAYQIRKREINEKQSEIDWRALRWSD
                     PRILRRTSKFSNR"
     gene            complement(585..>3170)
                     /locus_tag="VG754_03870"
     CDS             complement(585..>3170)
                     /locus_tag="VG754_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HWF18374.1"
                     /translation="GRICVLEDNDGSGIFDSRTVYAENIANPTALTCYAGGVFVAASG
                     QIIYLKDTQGNGVADARREVFKSFGDATNGANGQVVITGMEWGLDNRIHVVTTGYGGD
                     VISSSLPSQSVALSEGSFSFDPRTFALAAESGSGASGLSFDDHGQRVICTPTGQIQVI
                     WREARYAARNPFYEMPANLETTNDDRAAFIYPLVHGGIPVNVRTADPNSGIPLPVPFS
                     AAGGLAIYHGNAFPSDYWGDAFIADAAANVIHRAKLRQNGPALLAQRAADEPGVEFLA
                     GRDNSFEPTQVINAPDGAIYITGLSHNQELIAANKANAVLAAAPARGRIYRVVPVNFK
                     QPKFTPLGKLSAEQLAATLRNPNGWQRDTASRLLYEREDHATVVPLIRVLFDPLASPL
                     ARMRALCALDGIELNVQGRPTRALLESHLLKALNDPDDRVRERAVLLSEKFVSKAGTW
                     SEELWGQLSRMGSDPSPQVRWQLAFTLGQGHNSGRAQALANIIRTDPRAFPAALTSLS
                     DDAAEVFGLTVGDGRIRNDADGQNGLNALIQIVGQKNAPPEIAQVLNTLQGLADPGTA
                     FKMALELENGMAQADASLFSSPSAQAALPIFARAASVAVDLGADSSARVEALQLLASG
                     SAVDASIPVTLARQWLNFDSTMRRGAVAAMLLRPEWTSGLLATVQMGLIPRTDLDLEQ
                     IRFVLAYPNAELRGLAQILFANWNGLERQAVVNRYSAVPQMNGNPERGHAIFLKNCAV
                     CHQTADEPTARFITYSLVEASKADKQKILDAILTPNRPTPSTHPEWLFQMRNGELVTG
                     FVGSENAKGYAISDLSGAPHYLARSDITSQKNLGISGMPDDWENLLNAQDMADLLQYL
                     TAGRK"
BASE COUNT          672 a          905 c          908 g          685 t
ORIGIN      
        1 tgccacccaa agccacgaca aattatcggc tgttgaagtc atttatttgc tcgttgtttc
       61 tatcgcgcta ctaggatttt tgtggttttt ggcttgggtt atatggactc aatatatatg
      121 aaatcggggt tgacactcgg aaaatgcgcg caacatttca aactgctttg gttgcgtaag
      181 ttaattggca aaccccgcgt tccttacaat cgcggtttgc gaatccgagg ctcgtcgcat
      241 ccagaatttt atgacgctga tttatcactc ccggcagcag aaagcaacgc tttccgtttc
      301 cagtcggagt ttgtggattc agctgacaac acggtcaagg tcggcgtggc atatcaaata
      361 agaaagcgag aaataaatga aaaacaaagt gaaattgatt ggcgggcctt acgatggtca
      421 gacccgagaa ttttacggcg cacctccaag ttttcaaatc gatgaacgca ttcaggaaag
      481 aaatggccag tggagagttt atcattcgtc ggtatatcgg ctaaaaaccg aatcaccatt
      541 ggaatacacc tttgacgatt caaattaagg caatgccctt tgcttcattt gcgccccgcc
      601 gtcaggtatt gcagcaaatc ggccatgtcc tgcgcgttca acaagttttc ccaatcgtcc
      661 ggcatcccgg aaatgccgag gttcttctgg ctcgtgatgt cggaacgcgc gagataatgc
      721 ggagcgcctg aaaggtccga aatggcgtaa cccttcgcgt tttcactgcc aacaaatccg
      781 gtcaccaatt cgccgttgcg catctggaaa agccactccg gatgcgtcga tggggtggga
      841 cggttcggcg tgaggatggc gtcgagtatc ttttgtttgt ccgccttgga cgcctcgaca
      901 aggctgtaag tgatgaaccg cgcggtgggt tcatcggcgg tttgatggca cacggcgcaa
      961 ttttttagaa agatggcgtg cccgcgttcc gggttcccgt tcatctgcgg gaccgcgctg
     1021 tagcgattca ccaccgcctg gcgttccaag ccgttccaat tcgcaaacag gatctgcgca
     1081 agcccgcgca gttccgcatt cggataagca agcacaaaac ggatttgttc caggtccaga
     1141 tcggtgcggg gaatgaggcc catttgcacc gtggcgagca aaccggaagt ccattccggc
     1201 cgcagcaaca tcgccgccac ggcgcctctg cgcatggtgg aatcgaagtt cagccattgg
     1261 cgggcgaggg tgacgggaat ggaagcatcc accgccgagc cgcttgccaa aagttgcaac
     1321 gcttcaaccc gcgcggagga gtccgcgccc agatcgacgg cgacggaagc ggcgcgcgca
     1381 aaaatcggaa gggccgcctg cgcgcttggg cttgaaaaca acgaagcgtc ggcctgggcc
     1441 atgccgtttt cgagttcgag ggccatcttg aatgcggtcc ctggatccgc caagccttgc
     1501 aaggtgttca acacctgcgc gatttcgggc ggcgcgtttt tttggccgac aatttgaatc
     1561 agcgcgttca aaccattttg gccgtcggca tcgttgcgaa tcctcccgtc gccgaccgtc
     1621 aagccgaaca cttccgccgc atcgtcgctc aaggatgtca gggccgccgg gaaagcgcgc
     1681 ggatctgtgc ggatgatgtt cgccagcgct tgcgcgcggc cggaattgtg cccttgcccg
     1741 agcgtgaacg cgagctgcca gcgcacttgc ggcgacgggt cgcttcccat ccggctcaac
     1801 tggccccaca attcttccga ccacgttccg gctttcgaca cgaatttttc ggagagcaac
     1861 accgcgcgtt cgcgcacgcg gtcgtcgggg tcattcaacg cctttaacaa atgcgattcc
     1921 aaaagcgcgc gcgtgggacg gccctgcacg ttcagttcga ttccgtccaa ggcgcacaag
     1981 gcgcgcatcc gcgccagggg tgacgcgagc ggatcgaaca acacgcggat caatggcacg
     2041 acggtcgcgt ggtcctcgcg ttcgtacaaa agccgcgatg ccgtgtcgcg ctgccagccg
     2101 ttcggattcc gcagcgtggc ggccaattgt tcggcggaca gtttgcccaa cggcgtgaac
     2161 ttgggttgct tgaaattgac gggcaccacg cgataaatgc gccctcgcgc tggagcagcg
     2221 gcgaggactg cattcgcttt gtttgcggca atcagttcct ggttgtgcga aaggccggtg
     2281 atgtaaatcg ctccgtccgg ggcgttgatg acttgcgtcg gttcgaacga attgtcgcgg
     2341 cccgcaagaa attccacgcc cggttcgtct gcggcgcgtt gtgcgagcaa cgcgggccca
     2401 ttttgccgga gcttcgcgcg atggattaca tttgccgccg cgtccgcgat aaaagcatcg
     2461 ccccaataat ctgacggaaa cgcgttgcca tgataaatcg caagaccacc cgccgcggaa
     2521 aatggaacag gcagcggaat gccagaattt gggtccgctg tgcggacgtt gacgggaatt
     2581 ccgccatgca cgagcggata gatgaacgcg gcgcgatcgt cgtttgtggt ttcaaggttc
     2641 gcgggcattt cataaaacgg atttcgcgcc gcataacggg cttcacgcca gatgacctga
     2701 atttgccctg taggagtaca gatgaccctt tgtccgtgat cgtcgaagct cagtcccgat
     2761 gcgcccgagc cgctttcggc tgcgagcgca aatgttcggg gatcaaagga aaaacttcct
     2821 tcgctcagcg ccacggattg actcggcaaa ctgctcgaaa tcacgtcacc gccgtaaccg
     2881 gtcgtgacga catggatgcg attgtccaaa ccccattcca tgccggtgat gaccacctgg
     2941 ccgttcgcgc cgttggtggc atcgccaaaa cttttgaaca cctcccgccg cgcgtccgcg
     3001 accccattgc cctgggtgtc cttcaaatag atgatttgtc ccgacgccgc gacgaagacc
     3061 ccgccggcgt aacaggtcaa cgcggttgga tttgcgatgt tctccgcgta aaccgtccgc
     3121 gaatcgaaaa tcccgctgcc gtcattgtct tccagcacgc aaatgcggcc
//
LOCUS       DASZMM010000155         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2538327, whole genome shotgun sequence.
ACCESSION   DASZMM010000155 DASZMM010000000
VERSION     DASZMM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538327"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1623
                     /locus_tag="VG754_03875"
     CDS             316..1623
                     /locus_tag="VG754_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18375.1"
                     /translation="MGSARAGSLLDWQRAVTGGTHYSLSRQFVIQDIPAAENFSSRLL
                     NETNLMELDPTLLAVSCERIKKNLLLQLGVRDQWRGKIYIALHRAQFSDEPVSVTPER
                     FDREWDYKLDMPDRVERSRLVSAIVEVLLLEMADRGAERSTEIPAWLSQGMSREVTFT
                     SEEELVIEKPHSMENGLGMNRLTRTDLMGNPLLQAHAELLAMPPLTLEDLSWPKAGQF
                     DGDAAEAYRSSAQLFVHELLQLNDGHDCFRAFLSELPQHFNWQMAFLRAFRSHFGTQL
                     DLEKWWALRLVEFTGRDLAQTWRADESWQKLDEIVRPAVEVRTDANEMPLHSQVTLQA
                     IIRDWDVPRQRQMLQEKLQQLLILRLHVSQEFVYLVDDYRRTLDGYVKKREKSRIAFG
                     KTLSDDLARETIRTLDALETRRQQLRPNPQIPPAITANTSATS"
     gene            1705..2943
                     /locus_tag="VG754_03880"
     CDS             1705..2943
                     /locus_tag="VG754_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HWF18376.1"
                     /translation="MSIIFSEAGHVADGETTKLNLLKQFHSSGRGFWSHVSDADWNDW
                     RWQLKNRITSLEQLQKLMPTLSPEEHAGTVLANSKLALAITPYFFNLIDPADENCPIR
                     WQVIPRIEETHTAPWEMTDPCGEDSHSPVPGLVHRYPDRVLFLVTDRCASYCRYCTRS
                     RLVSNASGYDFHPEFEKQIDYVRRNTSVRDVLLSGGDPLLLSDEKLEYLLSRLRAIPH
                     VEFLRIGSRIPIFLPQRITPELCAMLKKYHPLFMSIHSNHPRELTTEVREGLGRLADA
                     GIPLGNQSVLLRNVNDDLTVMRALVQKLLMCRVKPYYLYQCDLIAGSAHLRASVRKGL
                     EVMEGLRGHTTGYAVPQYVIDAPGGGGKVPVNPDYVLSRNADRVIIRNFEGKIFEYPE
                     ATDGTPLVKAPLEIEEPELV"
BASE COUNT          739 a          918 c          978 g          787 t
ORIGIN      
        1 ggagaaatgg agtgcggatt gtagattgcg gagtgtccgt tgcgaactta atcaagcatc
       61 aagagtccat gatcgagcat ccagcatcgg tttgttaagg ctaataaagg ctaattaagg
      121 gtaattaagg ctaagaagga aaaaatcttt ttgctgaccg tgtcgcttaa tgtcgcttgg
      181 cgttggaact gaagcgcggc tgtcgcgctt gagtccggct tttccttgct tgtcagtcgc
      241 gcggaaatgc ggtggaatgg ggttggattg atgtttaaac gccggttcat tttttgcctt
      301 tgcctgacgg caatcatggg gagcgctcgc gccgggtcgc tgctggattg gcaacgcgcg
      361 gtcaccgggg gaacgcatta cagtctcagc cgccagttcg tcatacagga cattcctgct
      421 gccgaaaatt tctcctcgcg gttgcttaat gaaaccaact tgatggaatt ggatcccacg
      481 ttgctggccg tctcttgcga gcgcatcaag aaaaatctgt tgttgcagtt gggcgtccgc
      541 gatcaatggc gcggcaagat ttacatcgcc ttgcatcgcg cgcaattcag cgacgaaccc
      601 gtcagcgtca cgcccgagcg ttttgaccgc gaatgggatt ataagctcga catgccggac
      661 cgggtggaac gttcgcgatt ggtttcggcg attgtcgagg tgttgttgct tgaaatggcg
      721 gaccgcggcg cggagcgttc gactgaaatt ccggcgtggc tcagccaggg aatgtcgcgc
      781 gaagtgacgt tcacgagcga ggaagaattg gtgatcgaaa aaccgcacag catggaaaac
      841 ggccttggca tgaaccgcct cacacgcacg gacctgatgg gcaacccgct ccttcaagcc
      901 catgcggaat tgctggcgat gccgccgttg acgctcgagg atttaagctg gcccaaggcc
      961 gggcaattcg acggcgacgc ggcggaagcg tatcgcagca gcgcgcaatt gttcgttcac
     1021 gagttgctcc agcttaacga cgggcatgat tgtttccgcg cgtttttgtc cgagttgccg
     1081 cagcatttca actggcagat ggcgttcctg cgggcgttcc gttctcattt cggaacgcag
     1141 ctcgacttgg aaaaatggtg ggccttgcgc ctcgtggaat tcaccgggcg cgatcttgcg
     1201 caaacctggc gggcggacga aagctggcaa aaattggacg aaatcgtgcg tcctgccgtt
     1261 gaggttcgca ccgacgccaa cgaaatgccg cttcattcgc aggtgacttt gcaggcgatc
     1321 atccgtgatt gggacgttcc gcgtcaaagg cagatgttgc aggagaaatt gcagcaactg
     1381 cttatcttgc gcctgcacgt ttcgcaagag ttcgtttatc tggtggacga ttatcgccgc
     1441 accttggacg ggtatgtgaa aaagagggag aaatcccgga ttgccttcgg gaaaactctt
     1501 tctgatgact tggcgcgaga aaccatccgc accttggatg cgctcgaaac gcggcgtcag
     1561 caactgcggc caaacccgca aatcccaccg gccatcaccg ccaatacgtc tgcaaccagt
     1621 tgatagcaag cgtcttgcaa caaaaatgga aaattttccc ccgttccttg acaggttaaa
     1681 ggctgaattt atagtgcgtg aacgatgtca atcatctttt cggaagcggg tcacgtggca
     1741 gacggcgaaa ccaccaaatt gaacctcctt aaacaattcc attcctccgg gcgcggcttc
     1801 tggagtcacg tttcggacgc cgattggaac gactggcggt ggcagctcaa aaaccgcatc
     1861 acttccctgg aacaactcca gaaactgatg ccaacgctct cgcccgagga acatgcaggc
     1921 acggtcctgg ccaattccaa gctggcgctg gccatcacgc cttacttttt caacctgatt
     1981 gatcccgcgg atgaaaattg cccgattcgc tggcaggtca tcccgcgcat cgaggagacc
     2041 cacaccgcgc cttgggaaat gaccgaccct tgcggcgaag attcccattc gccggtgccc
     2101 ggtctcgtgc atcgttatcc cgaccgcgtg ctgtttctcg tcacggaccg gtgcgcgtcc
     2161 tattgccgtt attgcacgcg ttcgcggctc gtcagcaacg cgagcggtta cgattttcat
     2221 cctgagttcg agaagcagat cgattacgtc cgccggaaca cttccgtgcg cgatgttttg
     2281 ttgagcggcg gcgatccgtt gctgttgagc gatgaaaaat tggaatactt gctcagccgg
     2341 ttgcgcgcga ttccgcatgt tgaatttttg cgcatcggtt cgcgcatccc gatttttctg
     2401 ccgcaacgca tcacgccaga actttgcgcg atgctcaaga aatatcaccc gctgttcatg
     2461 agcatccaca gcaaccatcc gcgcgaactc acgacggaag tccgcgaagg tcttggacgc
     2521 ctcgccgatg ccgggattcc gctcggcaac caatccgtgc tgttgcgcaa cgtaaatgac
     2581 gatttgaccg tcatgcgcgc gctcgtgcaa aaacttctga tgtgccgtgt gaaaccgtat
     2641 tatttgtatc aatgcgattt gatcgccggt tctgcgcatt tgcgcgcgag cgtgcgcaag
     2701 ggattggaag tgatggaagg attgcgcggc cacacgacgg gttacgccgt gccacaatac
     2761 gtcatcgatg cgcccggcgg cggcggcaag gtgccggtga atccggatta cgttttgagc
     2821 cgcaacgctg accgcgtcat catccgcaat ttcgaaggga aaattttcga atatcccgaa
     2881 gctaccgacg gtacgcccct cgtgaaagcg ccactggaaa tcgaagagcc ggagttggtg
     2941 tgagtgggcg tatctcagtt tcctgcacga cggagtaatt gaacgtttgc tccccctcac
     3001 cggcccaatg gcttcccaag cccgccacaa gtcagtttcc ccaaaggaga cgaacgcttc
     3061 gcgttacgtg gtcccttggg gagagggagc aacgcatccc gtcgtggttg caatctgacg
     3121 gcgttcaatt tgtcgagcag cggccgaatg gttcccccct ctccttgggg agggctgggg
     3181 tggggagaca gcgatgtgcg ccggctcatg aacacaattc gcaagagccc agaaatgtgc
     3241 aaaatagtga gttgcgccct ttattatcgc gacagaaatt ttggtgacta aaacgcaaag
     3301 gggtcatgaa gaggcaaccc atcgtcggcc tctttgcgtc cttttgcggc tgaattgagt
     3361 ttttgtttta ccggcttttg cgctttttag cggacaaatt ttttgcctcg atgctaaagg
     3421 ag
//
LOCUS       DASZMM010000156         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3623177, whole genome shotgun sequence.
ACCESSION   DASZMM010000156 DASZMM010000000
VERSION     DASZMM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623177"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /gene="nadC"
                     /locus_tag="VG754_03885"
     CDS             <1..573
                     /gene="nadC"
                     /locus_tag="VG754_03885"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWF18377.1"
                     /translation="LSAERVALNFVQRLSGVATLTAQFVAAIEGTGAKILDTRKTTPG
                     WRRFEKYAVTCGGGHNHRIGLYDMVLIKDNHLAALRDEKPNAIAVAVQRARAQFPKLK
                     IEVEADTLEQVRQAVSAGADFILLDNMTLAQLREAVKIVQGRAKTEASGGVNLTTVRA
                     IAETGVDFISAGALTHSARAVDIALDFEEP"
     gene            570..1550
                     /locus_tag="VG754_03890"
     CDS             570..1550
                     /locus_tag="VG754_03890"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWF18378.1"
                     /translation="MTIDTQILAALRGARDVGVAGTELSQKLGISRAAIWARIEELRG
                     LGYEIEAGPHLGYRLLSAPDLLHADDLLAALGKTRVIGRDIRVFEETTSTNDVVEKLA
                     RDDVREGAVVFAESQTRGRGRLGRKWISPARKGLWFSVLLRPPMRPATVTQLTIVAAT
                     ALFRAIQSQTGLVPEIKWPNDILLRGKKTAGILTELSAEPDKVKYVILGVGIDVNLSA
                     AEFPADLRKLATSLRIETGQRQDRAELAVKILRQLDRDYGRVCAGHFEVVADEWESHC
                     TTLGRRVAIRIGERTIQGTAESLDTDGALLLRTQHGRLERIIGGDVTVEK"
     gene            1554..2318
                     /locus_tag="VG754_03895"
     CDS             1554..2318
                     /locus_tag="VG754_03895"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWF18379.1"
                     /translation="MILLLDIGNTHTHLGLANAKRVVKQTNISTAHWFDGGAEKLVKK
                     FIGRASLEGAALCSVVPRVTPRARAAIKRTWRLDCLVLTPKTVLGVGIDYPKPNSIGP
                     DRLANAVAVKHHFGAPSVVVDFGTAVTFDVVDRAGNYVGGIIAPGLAVMTDYLHEKTA
                     LLPRIKITEENAVVGKNTAQAMLIGAVHGYRGLIRELIAELKKDLRATRLPVVATGGY
                     AKLMASKLPEITTVDPLLTLEGLRLVWQRKMLDTEC"
     gene            2418..>2904
                     /gene="trpA"
                     /locus_tag="VG754_03900"
     CDS             2418..>2904
                     /gene="trpA"
                     /locus_tag="VG754_03900"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWF18380.1"
                     /translation="MNRINERFARLKRDAQKGFVVYIGAGDPNLEATRQLALAFDKAG
                     VDVLELGVPFSDPLADGLVNQLAAQRGLESGTTPAKVLETVRSIRKDSQVPIVFYIYF
                     NLLHRYGFKKFIEDAAQSGVDGLLVLDLPPEESENYETMMRETGLCVIYLIAPTTPED
                     RM"
BASE COUNT          627 a          804 c          872 g          601 t
ORIGIN      
        1 ctttccgccg aacgagtggc gttgaatttt gtccagcggc tttccggcgt ggccactttg
       61 accgcgcaat tcgttgcggc cattgaggga acaggcgcaa aaattctcga cacgcgcaag
      121 accacgcccg gctggcgtcg cttcgaaaaa tatgccgtca cttgcggcgg cggacacaac
      181 catcgcatcg gcttgtacga catggtgctc atcaaggaca accacctggc ggcattgcgg
      241 gacgaaaagc cgaatgcgat tgccgtagcc gtgcaacgcg cgcgcgcgca atttccaaaa
      301 ttaaaaatcg aagtcgaggc cgacacgttg gaacaagtga gacaggccgt gagtgcaggc
      361 gcggatttca ttctgctgga caacatgacg ctggcacaat tgcgcgaggc ggtgaaaatc
      421 gtgcaaggcc gcgcaaaaac cgaagccagc ggcggggtaa atctaacaac ggttcgcgcc
      481 atcgccgaaa cgggcgtgga ttttatttca gccggcgcat tgacccattc cgcgcgcgcg
      541 gtggatattg cgctcgattt tgaggaacca tgacgattga cacccagata ctcgcggcgt
      601 tgcgcggcgc gcgtgacgtt ggcgtggccg gcacggagct ttcgcaaaag ctcggcatca
      661 gccgcgctgc gatttgggcg cgaattgagg aattgcgcgg gttgggttat gaaatcgaag
      721 ccggcccgca tctcggctac cgactgctga gcgcgcctga tttgttgcac gccgatgatt
      781 tgcttgcggc tttgggcaag acgcgcgtca tcgggcgtga catccgcgtt ttcgaggaaa
      841 ccacttcgac caatgacgtt gtcgaaaaac ttgcgcgtga cgatgtgcgc gaaggagctg
      901 tggttttcgc ggaatcgcag acgcgcggtc gcgggcggct cgggcgcaaa tggatttctc
      961 cggcgcgcaa gggactttgg ttttctgttt tgctgcgtcc gccgatgcgt cccgcgacgg
     1021 tcacacaact gaccattgtt gccgcgacag cgttgttccg cgccatccaa tcgcagaccg
     1081 gccttgtgcc tgaaatcaaa tggccgaatg acattttgct gcgcggcaaa aaaacggcgg
     1141 gcatcctgac tgaattgagc gccgaacctg acaaggtgaa atatgtcatc cttggcgtcg
     1201 gcattgatgt gaatttgagc gctgccgaat ttcccgccga cttgcgcaag ctggccactt
     1261 cgttgcgcat cgaaactggc caacgacagg accgcgccga actcgcagtc aaaatccttc
     1321 gccaacttga ccgcgattac gggcgcgttt gcgccgggca ctttgaggtc gtcgcggatg
     1381 aatgggagtc gcattgcacg acccttggca ggcgggttgc cattcgcatc ggcgagcgca
     1441 ccatccaggg cacggcagaa tcgctcgaca cagacggcgc gttgctgctg cgcacccagc
     1501 atggccgcct ggaacgaatc attggcggcg acgtgaccgt tgaaaaataa aacatgatac
     1561 tgctgctcga tatcggcaac acgcacacgc atctcggttt ggcgaatgcc aaacgcgtgg
     1621 tcaaacaaac caatatttcc actgcgcatt ggttcgatgg aggcgctgag aaattggtga
     1681 aaaaatttat tggacgtgct tcgctggaag gagcggcatt gtgcagcgtt gtgccccgcg
     1741 tcacgcctcg cgcgcgcgcg gcgatcaaac gaacgtggcg tttggattgt ctggtgctga
     1801 ctccaaaaac agttctcggc gtcggcattg attatcccaa gccaaacagc atcgggcctg
     1861 accgtcttgc caacgcggtg gccgtgaaac atcatttcgg cgcgccttcg gtcgtggtgg
     1921 atttcggcac ggctgtgacg tttgacgtcg ttgaccgcgc gggaaactat gtcggcggca
     1981 tcatcgcgcc gggcttggct gtgatgaccg attatctgca cgaaaaaact gcgctgttgc
     2041 cgcgcatcaa gatcactgag gaaaacgccg tcgtcggcaa aaataccgcg caggccatgc
     2101 tcatcggcgc ggtgcatggt tatcgcggct tgatccgcga attgatcgcc gaattgaaaa
     2161 aggacttgcg agcaacacga ttgcccgtgg tcgcgacggg cggttacgcg aagctgatgg
     2221 cgtcgaagct gccggaaatc acgacggtgg acccgttgct cacgttggag ggattgaggt
     2281 tggtttggca aaggaagatg ctggatactg aatgctagat gctggaaaga agcgaaagcg
     2341 ttcagccaga atccagaatc tggcatcaag catcctcttc cttgaacttg cccgcaattc
     2401 ccggcaaact tttccgaatg aaccgcatca acgaacgatt tgcccggctc aagcgcgatg
     2461 cgcagaaagg cttcgtcgtc tatattggcg ccggcgatcc gaaccttgaa gcgacccggc
     2521 aactcgcgct cgctttcgac aaggccggcg tggatgtgct tgaactcggc gtgccattca
     2581 gcgacccgtt ggccgacggc ctcgtcaacc agcttgccgc gcaacgcggt ttggaatcgg
     2641 gcacgactcc ggcgaaagtt ttggaaacgg tgcgctccat ccgcaaagat tcgcaggtcc
     2701 cgattgtctt ctacatttac ttcaacctgc tgcatcgtta cggattcaaa aagttcatcg
     2761 aagacgccgc gcaatccggc gtggatggtt tgctcgtgct cgatttgccg ccggaagaat
     2821 ctgaaaatta cgagacgatg atgcgcgaga ccggtttgtg cgtgatttat ctcattgcgc
     2881 cgacgacgcc cgaagaccgc atgg
//
LOCUS       DASZMM010000157         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1442545, whole genome shotgun sequence.
ACCESSION   DASZMM010000157 DASZMM010000000
VERSION     DASZMM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442545"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2186)
                     /locus_tag="VG754_03905"
     CDS             complement(<1..2186)
                     /locus_tag="VG754_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HWF18381.1"
                     /translation="MTSFSALAARQPYELKSGERVVLIGDTLIEREQSYGYLEERMTV
                     RYPDRDVQIRNLGWSADTPEGISRASFDWDKPGKGFELLTNQIAAVQPTLVIIGYGMA
                     NSFQGEAGLPQFKTNLLKLVDAIQTICTNKPRFVFLSPIRHENLGFPLPDPAEHNKQL
                     ALYTRTVLEAATERQGLFISLYNNLLGDGTESHPPRPFTDDGIHLTGYGYLRMTEAVE
                     KGFAWEPNFWRVRITADGEVTPGSYGTSVTNLTRTKDKIQFTSLDEQLVSSYISDKSG
                     RIPSADAPSLIQIDGLKRGRYQLKVDGKVIAIATDKEWADGVVIERGPQFDQAEKLRN
                     AILKKNELYFDRSRPENETYLFGFRKHEQGQNAKEIPEFDPLIAEQEAKIAKLRKPMP
                     HMYELTRTRKSFVAETSKPTKAQTEVASTAKPLEPLPNFDVAPGFEVSLYAESPMLAK
                     PTQMNFDPSGRLWVASSSVYPQIKPGQVADDKIIVLEDTKGIGKVDKSTVFVDGLMIP
                     TGVEPGDGGVYVGQGTQLLFFKDTHGTGKADEKRIVLSGFGTEDTHHLVHTLRWGYDG
                     QLYFDQSIYIHSHFETPNGVERLNSGGIWHLRPQTMQLGIFLRGFCNPWGHEFDKYGQ
                     SFVTDGAGGQGVSWGIPGATYFTYADMRRELQSISPGSYPKFCSLELIRSKQFPDDWQ
                     GNAITCDFRAHRVVRFAMDEKDSGYATREMPDLLRTTNVTFRPIDV"
     gene            2497..3261
                     /locus_tag="VG754_03910"
     CDS             2497..3261
                     /locus_tag="VG754_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18382.1"
                     /translation="MDHRLIATLLLSVFSFVLVMGGFSVYMVLRWRHLRGSHIPQGII
                     KVPPLQSESPPGFRHFALKRPNCWLAIKNRNLLAVQSALAVHNAKPCSCLEGLSGDGE
                     QKLFISPPISGWILVIGSALPDPCEDVDACFRFLLEVSRKLGHVQFFFANAMLNYHAW
                     AQADGGHINRGYAWAGRTLWTQGAMTSAESDLGMRCFDYSDPADVPTFSAPDASCNNS
                     DKVHLLAARWSVDPEDLDERLIEHEWGIVGEPSRLF"
BASE COUNT          718 a          924 c          966 g          815 t
ORIGIN      
        1 ttcacgtcaa tgggacggaa agtgacgttc gtggtgcgga gcaaatccgg catttcgcgc
       61 gtggcatagc cggaatcttt ctcatccatc gcaaagcgca ccacgcgatg cgcgcgaaaa
      121 tcgcaggtga tggcgttccc ctgccagtca tcaggaaatt gcttgctgcg gataagttca
      181 aggctgcaaa attttgggta agaaccggga ctgatgcttt gcagttcgcg gcgcatgtcg
      241 gcgtaggtga aataagtcgc gccgggaatg ccccaactca cgccctggcc gcctgcaccg
      301 tcagtgacga aggattggcc gtatttgtcg aattcatgcc cccacggatt gcaaaaaccg
      361 cgcagaaaaa tgccaagctg catcgtctgc ggacgcagat gccagatgcc gccgctgttc
      421 aagcgctcga cgccgttggg cgtttcgaaa tggctgtgga tgtaaatgga ttggtcaaaa
      481 taaagctggc cgtcgtaacc ccagcgcaag gtatgcacaa gatgatgcgt gtcctcagtg
      541 ccgaaaccgg aaagcacgat gcgtttttcg tccgccttgc cggtgccgtg cgtgtccttg
      601 aaaaaaagca gttgcgtccc ctgccccaca tagacgccgc cgtcgcccgg ttccacgcca
      661 gtcggaatca tcaacccatc cacgaatacg gtggacttgt ccaccttgcc gatgcccttg
      721 gtgtcttcca gcacgatgat tttgtcatcc gcaacctggc cgggcttgat ttgtggataa
      781 accgaagaac tcgcgaccca aaggcgccca ctcggatcga aattcatctg ggtcggtttt
      841 gccagcatcg ggctttcggc ataaaggctg acttcaaatc cgggcgcaac gtcaaaattt
      901 ggcaaaggct caagcggctt ggcggtcgaa gcgacttcgg tttgcgcctt ggtcggcttg
      961 ctcgtttctg caacgaagga tttgcgggtc cgcgtcaatt cgtacatgtg cggcatgggc
     1021 ttgcgcaatt tcgcgatctt cgcctcctgc tccgcgatca acgggtcgaa ctcaggaatc
     1081 tccttcgcat tctggccttg ttcgtgtttt cggaaaccga aaagatacgt ctcgttttcc
     1141 gggcgtgagc ggtcgaaata aagttcgttc tttttgagaa tggcattgcg caatttctcg
     1201 gcctgatcga attgcggccc gcgctcaatc accactccat ccgcccattc cttgtcagtc
     1261 gcgatcgcaa tcactttgcc gtctactttc aattggtagc ggccgcgttt gagtccatca
     1321 atttggatga gtgacggcgc atcggcagaa gggatgcgtc cgcttttgtc ggagatgtaa
     1381 gagctgacga gttgttcgtc gagcgaagtg aattggattt tgtcctttgt gcgcgtcagg
     1441 ttcgtgacgg atgtgccgta actgccgggc gtgacttcgc cgtccgcagt gattcgcacg
     1501 cgccagaaat tcggttccca cgcaaacccc ttttccaccg cttcggtcat tctcaaatag
     1561 ccataacccg taagatggat gccatcgtcc gtgaacggac ggggcggatg cgactcggtg
     1621 ccgtcgccga gcagattgtt gtaaagcgaa atgaaaagac cctggcgttc ggtggcggct
     1681 tccaaaaccg tgcgcgtata aagcgccaat tgtttgttgt gttcggcggg atcgggcagc
     1741 ggaaacccaa gattttcatg gcgaatcgga ctcaagaaaa cgaaccgcgg tttgttcgtg
     1801 cagatggttt ggatggcatc cacgagcttg agcaagttgg tcttgaattg cggcaacccc
     1861 gcctcgcctt ggaaagaatt tgccatgccg tagccgatga tgacgagcgt cggctgcacg
     1921 gcggcaattt ggttcgtcaa taattcgaaa cctttgcccg gcttgtccca atcgaaactc
     1981 gcgcgcgaaa tgccttccgg cgtgtcggcg ctccagccga ggttccggat ttggacgtcg
     2041 cggtccgggt agcggacggt catgcgctct tccagataac cgtaggattg ctcgcgctcg
     2101 atcaaggtgt cgccgatgag aaccacgcgc tcgccagatt taagttcata cggttgccgc
     2161 gccgccaagg cggaaaatga ggtcaaagca attgctgcga ggaagatcag gcaccgaaaa
     2221 aatttctgtg gtttaaaaca ggcggctttc attcaacgac acgcaaaggt gaattaatga
     2281 atggcaagta aaacggtgtc tgcgaaaaaa ttcaacagaa tatgcggtcg cccttggatt
     2341 tccaatgaag cgggaattgg caggaattga aaatttggcc tccaaaactt cattcattcg
     2401 cgcggagctt gtccatgggg tgaaaaactt ttcgattcaa acttcttttc aattgaattg
     2461 ggaagaacga ggagtaagtt gcatcgtatg ggtgcaatgg atcacagact gattgcgaca
     2521 cttcttttgt cggtcttttc cttcgtgctc gtcatgggag ggttctcggt ctatatggtt
     2581 ctccggtggc gtcatttgcg cggaagtcac atcccgcaag gcatcatcaa ggtgccgcct
     2641 ttgcaatctg aaagccctcc cggtttccgt catttcgcct tgaaacggcc caattgctgg
     2701 cttgcgatca agaatcgcaa ccttttggcg gtgcaatccg cgctggccgt ccacaacgcc
     2761 aagccttgtt cctgccttga aggattgtcc ggtgacggcg agcaaaagct cttcatttcg
     2821 ccgccgattt ccggctggat actcgtcatc ggctccgcgt tgcccgaccc ttgcgaggac
     2881 gtggatgcct gtttccgttt tcttctggaa gtgagccgca agctcgggca tgtccaattc
     2941 ttcttcgcca acgcgatgtt gaattatcat gcctgggcgc aggcggacgg agggcacatc
     3001 aatcgcggct atgcgtgggc cggcaggacg ctttggacac agggcgcaat gacgtcggcg
     3061 gaatccgact tgggaatgcg ctgcttcgat tattccgacc cggcggacgt cccgacattc
     3121 agcgcgccgg atgcaagctg caacaattcg gacaaggtgc atctgctcgc cgcgcgctgg
     3181 agcgtggatc ccgaggacct tgacgagcgt ctcatcgagc acgaatgggg aatcgtcggc
     3241 gaaccgtcgc gcctgtttta acctttgcgt gcggtttgtc gagcagcggc agaatggttc
     3301 accctctcgg gcatttcttg acactgttct tgagaatgta cgggtacggt ttggatgacg
     3361 ctcgccctca cccaaaccct ctcccccaag agagggcttt cactgtttcc cgctgttcgg
     3421 aac
//
LOCUS       DASZMM010000158         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1443010, whole genome shotgun sequence.
ACCESSION   DASZMM010000158 DASZMM010000000
VERSION     DASZMM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443010"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VG754_03915"
     CDS             <1..475
                     /locus_tag="VG754_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWF18383.1"
                     /translation="VWPQLLDVFPGKNVIERSSMNSWDSMEFRKAVEATGRKNILICG
                     LWTEVCVTWPTIEMMGAGYNVYVVEDCCGATSPAAHEAALSRMVQAGAVRLTTIQALL
                     EWQRDWKNREHYDALMVLLKQQAGAYGAGLEYAYTMVHHAPQSAQKPQIVPKKAA"
     gene            501..1229
                     /locus_tag="VG754_03920"
     CDS             501..1229
                     /locus_tag="VG754_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HWF18384.1"
                     /translation="MKTKAFALGLTLACLVATAGAADSPAPKESVFATKPLGIRVSIK
                     MDGPYMEAADLQIICLFKHQASGDTYQGAAKETDAHLGSILSALRNRGEFVGELGETF
                     LFTPPKSSIPAKRFMVIGLGEEKDLSLDSLKVVGHIAAREAVRLKAKHVAWAPVIRDE
                     GDSTIEVGVGDRAFAEAMLSAYDTEKRLQDQGLAPKFSIESFVIEAGPSFFDSAVKEV
                     GEGIESRVTALKQRDTTPYASEQH"
     gene            1264..2631
                     /locus_tag="VG754_03925"
     CDS             1264..2631
                     /locus_tag="VG754_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWF18385.1"
                     /translation="MALASQVMAQDLTNSSENSYSPPAYQVLRFRENYQCLADPSLRQ
                     DIFDPIKYMQLGADTNFFLTLGGEVRERFEGMHDPDFGIHGSHDEYWLQRIALFTDFH
                     LGERVRVFVEGISGLDEGNTLPAPPVQRDPADLQFAFVDIFPYINGDEHVILRAGRFG
                     LSLGSGRLVATRAAPNIPFKFDGGQIIYTGPAWQATAFLTRPVKDSGHIDWDDRTVTF
                     WGLYSTHWFDAQKKNGADLYYLGIHRENGAYASGKGNEYRHSFGAREFGEWRGLDYNA
                     EEVVQTGTFGKESILAWTASLDSGYTWNTLFNPRLGIKADVASGDKNPNDGHQGTFDA
                     LFFKSGYFNDASLIRPENIIDLHPNLSLKLTSKFTVDGGADVFWRYSKNDAVYAVPGF
                     IALSAKSSASSYVGTAADLNLTWNIQRHLTWQASYVHFFSGSYIKGLGGGDVNYVSTT
                     LSFLF"
BASE COUNT          629 a          698 c          720 g          615 t
ORIGIN      
        1 tgtctggcca caattgctgg atgtgttccc gggaaaaaac gtcatagaac gttcttcgat
       61 gaattcctgg gattccatgg aatttcgcaa agcagtcgaa gccaccggtc gcaaaaacat
      121 ccttatctgc ggtttgtgga ccgaagtatg cgttacgtgg ccgaccattg agatgatggg
      181 cgccggctac aatgtttacg ttgttgaaga ctgctgcggc gcaacctcgc ccgcggcgca
      241 tgaagcagcg ctctcgcgca tggttcaagc cggtgcggtg cggctcacaa caatccaggc
      301 cttgctcgaa tggcagcggg attggaaaaa ccgagagcat tacgatgcgc tgatggtgtt
      361 gttaaagcaa caggctgggg cctatggcgc aggtctcgaa tatgcctaca caatggtgca
      421 tcacgcccct cagtcagctc aaaaaccgca aattgtgccc aagaaagccg cctgataccc
      481 cgataaaaac tggacccatt atgaaaacta aagcttttgc gttgggcctc acgttggcgt
      541 gtttggttgc aaccgcaggc gcagccgact cgccagcgcc gaaggaaagt gtgtttgcga
      601 ccaagccgct cggcatccga gtctccatta aaatggacgg cccttatatg gaggcggccg
      661 atctgcaaat catctgcctc ttcaaacacc aagcctccgg cgatacttac caaggcgcgg
      721 caaaggaaac ggacgcgcat ctcgggagca ttctttccgc cttgcggaat cggggcgaat
      781 tcgtcggtga actgggtgag acgtttcttt ttactccgcc caaaagttcc atacctgcca
      841 aacgttttat ggtgattggt ttgggagagg aaaaagactt gtccctggat tcgctcaaag
      901 tagttggcca catcgctgcc cgcgaagccg tgcgtttaaa agcaaagcac gttgcatggg
      961 ctccggtaat tcgcgacgag ggcgactcaa caatcgaagt cggggttggt gaccgcgcat
     1021 ttgcggaggc aatgctatct gcctacgaca cggaaaagcg tctgcaagac caaggactag
     1081 caccaaaatt cagcattgaa tctttcgtga tcgaagctgg tccgtccttc tttgacagcg
     1141 ccgtcaaaga agtgggcgaa ggcattgaat ccagggtgac cgcgctcaag cagcgagaca
     1201 ccacgcccta cgcttctgaa cagcactgag aaggcaatga agccgggctt ccttttgctt
     1261 tttatggcat tggcatcgca agtcatggct caggacctga caaattcgag tgagaattct
     1321 tattcgcctc cggcgtatca agtgcttcgt tttcgcgaga actatcagtg tcttgccgac
     1381 ccgagccttc ggcaagacat cttcgacccg attaaatata tgcaattggg agccgatacg
     1441 aatttctttt tgaccctggg cggcgaggtg cgggagaggt tcgaagggat gcacgatccc
     1501 gattttggaa ttcatggaag ccatgacgag tattggctcc agcgaatcgc gcttttcact
     1561 gattttcatt tgggcgagcg tgtacgggtg ttcgttgaag gaatatccgg cctcgacgag
     1621 ggtaacacgc tgccagcgcc tccggtgcaa cgcgacccgg cggatctgca attcgccttc
     1681 gttgacattt tcccgtacat caatggcgat gagcacgtga tactgcgagc aggcaggttt
     1741 ggattaagtc tcggctccgg tcggttggtc gcaacccgtg ccgctccgaa catccctttc
     1801 aagttcgacg gcggccagat catttacacc ggccccgcat ggcaggccac ggcttttctc
     1861 acacggccag taaaggattc tggccacatt gattgggatg accgcaccgt tactttttgg
     1921 ggcctatatt caactcactg gtttgatgcc caaaagaaaa acggcgccga cctgtattat
     1981 ttgggaatcc atcgcgagaa cggcgcgtat gcctctggca agggcaatga atacaggcat
     2041 tcatttgggg cgagggaatt tggtgaatgg cgtggcctgg attacaacgc cgaagaagtt
     2101 gtccagaccg gaacatttgg gaaggaatcc atcctcgctt ggacggcaag cttggacagc
     2161 ggttacacat ggaacactct ttttaacccg cggcttggaa tcaaagccga tgtggccagc
     2221 ggcgacaaaa acccaaacga tggccaccag ggaacctttg acgcgctctt tttcaaatcg
     2281 ggatatttta acgacgccag tcttatacgg cccgaaaaca taatcgacct tcatccgaat
     2341 ctgagcctca agctcacttc caaattcacg gttgatggtg gagcggacgt attttggcgc
     2401 tactcaaaaa acgatgccgt ctatgccgtg ccgggtttca tcgctctttc ggcaaaaagc
     2461 tctgcgtcga gctacgtggg gacggctgct gatttgaatc tgacctggaa cattcagcgc
     2521 catctcacct ggcaggcgtc ttacgttcac tttttttccg gcagctacat caagggactc
     2581 ggcggaggag acgtcaatta cgtttcaaca accttgagct ttcttttcta gtgccatgag
     2641 ccaaacgaga acacagccga tt
//
LOCUS       DASZMM010000159         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1973535, whole genome shotgun sequence.
ACCESSION   DASZMM010000159 DASZMM010000000
VERSION     DASZMM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973535"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2422)
                     /locus_tag="VG754_03930"
     CDS             complement(<1..2422)
                     /locus_tag="VG754_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF18386.1"
                     /translation="MKRTFLCWLMGIAILALVLPAIRAAEQASANIELPGMRADGSVL
                     LPNQWSLRPVGQQVPLGDFPVNIAVHPDGRFAAVLHSGHGAHEIIVVDINEAKVVSTT
                     KVNEAYYGIEFARSGARLYCSGSSDEVIHVFDFKDGVLTEIQPIQLCNPQERNIPCGI
                     AVSGNARDLFVANVWGQNIAKVDLMARTNVMEIAPIPGEEDKIERKTEAEKEDPDNPN
                     DPDLHAITKRAVLDPIKPDAPFPYACRLDDKRQLLFVSLWARSSVAVIDLKTFKVFAL
                     WPTQEHPNEMALTKSGRYLFVANANRNTVTVLDTTKGKTIETLYASFSSWALPGSTPN
                     SLALSPDENTLYVANACNNNVAVFDVSIIGKSRSLGFIPVGWYPTSVRVTPDGKHLLV
                     ANGKGLISKANPKGPQPGKKKTAETQSISELFPGTLSIIELPAKKQFRKQLAAWTEDA
                     YRCTPRESVTQVSPAHGNPVPAKVGRPSPIKYVIYIIKENRTYDQMLGDMTEGNGDPS
                     LCLFNERVTPNHHKLAREFVLLDNFYVDAEVSADGHEWSMGAYASDFVEKTWPLGYGH
                     AKSKKFPYPSEGRFPIAVPANGYIWDRAREAGVSYRSYGEFTFVGKNLKRPSFTRTAS
                     LEGHFDEWYRGFDTSYPDIKRAARFIEELKRLESEGDMPRLQIVRLPNDHTSGGVPGK
                     PTPIAQVADNDRGLGMVVEAVSHSKFWPETAIFVLEDDAQSGPDHVDAHRSPALVISP
                     YTRRGVTDSTMYSTSSLLRTIELILGLQPMTQYDAEATPMFNTFQTQADTRPYDSLPP
                     NVDLNERNP"
BASE COUNT          596 a          783 c          815 g          660 t
ORIGIN      
        1 cgggattgcg ctcattcaaa tccacattgg gcggcaacga atcgtaaggg cgtgtgtcag
       61 cttgcgtttg aaaggtgttg aacatcggcg ttgcctcggc gtcatattgc gtcatcggtt
      121 gcaatcccaa aatcaattca atcgttcgca acaaactgct cgtcgaatac atcgtcgaat
      181 ccgtcactcc gcgccgcgtg tatgggctga tcaccaatgc cggactgcga tgcgcgtcca
      241 cgtggtcggg gccgctttgc gcgtcatctt cgagcacaaa aattgccgtc tccggccaaa
      301 atttggaatg gctcaccgct tcaacaacca ttccaagtcc gcgatcgttg tccgcgactt
      361 gcgcgatggg cgtcggtttc cccgggacgc cgcccgacgt gtggtcgttc ggcagccgca
      421 cgatttgcag gcgcggcatg tcgccttcgg attcgagccg tttcaattct tcgatgaacc
      481 gcgcggcgcg tttgatgtcg gggtagcttg tgtcaaaccc gcggtaccat tcatcgaagt
      541 ggccttccaa cgaagcggtg cgagtgaagg acggacgctt gagatttttg ccgacgaaag
      601 tgaattcgcc gtagctgcga taactcacgc ccgcttcgcg cgcgcggtcc cagatgtaac
      661 cattggctgg aacagctatt ggaaaacgtc cctcggaagg atagggaaat tttttgctct
      721 tggcgtggcc gtaaccgagc ggccaggtct tctccacaaa atcgctcgcg taagcgccca
      781 tggaccattc atggccgtcg gcgctgactt cggcatcgac ataaaaatta tccagcaaaa
      841 caaattcgcg cgcgagcttg tggtgattgg gcgtgacgcg ctcgttgaac aaacaaaggc
      901 tcgggtcgcc attgccctcc gtcatgtcgc cgagcatctg gtcgtaagtc cggttttcct
      961 tgatgatgta gatgacatat ttgatggggc tgggacggcc aacttttgcc gggacaggat
     1021 ttccatgcgc gggcgaaacc tgcgtcacgc tctcgcgcgg cgtgcagcga taagcatctt
     1081 cagtccatgc ggcgagttgt ttgcgaaatt gttttttcgc cggcagttcg atgatgctca
     1141 acgtgccggg aaacaactcg ctgatggatt gtgtctcggc cgttttcttt ttgccgggct
     1201 gcggcccttt gggattcgcc ttggaaatca aacctttgcc gttcgcgacc agcagatgtt
     1261 ttccatccgg cgtcacgcgc acggaagtgg gataccagcc gacaggaatg aacccgagcg
     1321 aacggctttt gccgatgatg gagacatcga acaccgcgac gttgttgttg caggcattgg
     1381 cgacgtagag cgtgttttca tccggcgaca acgccaggct gttcggcgtc gaaccgggca
     1441 gcgcccagct cgagaaggaa gcgtaaagcg tctcgatggt tttgcccttg gtggtgtcca
     1501 acaccgtgac cgtgttgcga ttggcgttgg cgacgaacag gtaacggccc gatttcgtca
     1561 gcgccatttc gttcggatgc tcctgtgtcg gccacagcgc gaagactttg aatgtcttca
     1621 ggtcaatcac cgcgaccgat gaccgcgccc acaaactcac aaacaaaagc tgccgtttgt
     1681 catccagccg gcaggcatac ggaaacggcg cgtccggttt gatggggtcg agcaccgcgc
     1741 gcttggtgat ggcgtgcaaa tcggggtcgt ttggattatc gggatcttcc ttttccgctt
     1801 ccgttttgcg ctcgatcttg tcttcctcgc cggggatggg agcgatttcc atgacattgg
     1861 tgcgcgccat caaatccacc ttcgcgatat tttgtcccca gacattggcc acgaacaaat
     1921 cccgcgcgtt gcccgagacg gcgatgccgc agggaatgtt gcgttcttgc ggattgcaaa
     1981 gctggatggg ctggatttcg gtcagcacac cgtccttgaa atcaaaaaca tggatcactt
     2041 catcgcttga gccgctgcaa taaagccgcg cgccgctgcg ggcaaattca attccgtaat
     2101 acgcctcgtt taccttcgtc gtcgaaacaa ccttcgcctc gttgatgtcc acgacgatga
     2161 tttcatgcgc gccatgtcca ctgtggagca ctgctgcgaa gcggccatcc ggatgcaccg
     2221 cgatgttcac ggggaaatcc ccgagcggca cctgctggcc cacggggcgc aacgaccatt
     2281 ggttgggcag cagcaccgaa ccgtcggcgc gcatcccggg caattcgatg tttgcgctcg
     2341 cctgttccgc tgcgcgaatc gctggcagaa ctaacgcaag gattgcgatt cccatcagcc
     2401 agcataaaaa agttcgcttc ataaaatgtt tgccaacgat gccagacgct ggtgggacgg
     2461 taaagttaca ttcgggtttc tgactttggc aggcgcggat catgtcacaa aaaataattt
     2521 ttattttatc gcggtttatc ccggtcaagg ccggttggtg cggaaacatg cggaaaattc
     2581 cgactaatga agcggaaaca agcggaacaa atgcgcggat tgttgaggaa aaaaccggaa
     2641 aattgacgca aaaaaatcgt tttgggcagg gacggcgctt tagcccgtgt ggaaacaccg
     2701 ccccgcgctt ggagcatttc ggacaaaagg gaagattctg gcggatgaat ggccgttgct
     2761 cgtggacacc tggcggcgtc ctctgttgcg ctccttgact cacgcgggga agaccatttc
     2821 acatgccgga ttaattgtcc atctttttca caac
//
LOCUS       DASZMM010000160         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5279692, whole genome shotgun sequence.
ACCESSION   DASZMM010000160 DASZMM010000000
VERSION     DASZMM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5279692"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VG754_03935"
     CDS             complement(<1..577)
                     /locus_tag="VG754_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWF18387.1"
                     /translation="MQIESLKVFCDLAETESFTKAAQINNVTQSAVSQQISSLERQFK
                     SLLIERSKKKFRLTREGQVLYDFSKQIIQCYDSLHSKLQEIKDIISGTIRVATIYSIG
                     LHDLPPYIKKFLKSYPTVNVHVEYRRANQVYDDVLSNVVDLGLVAYPMRESKLETVSL
                     RKDPLVLICHPQHPFAKMKSVKLKGLSGQKFI"
     gene            complement(607..2118)
                     /locus_tag="VG754_03940"
     CDS             complement(607..2118)
                     /locus_tag="VG754_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18388.1"
                     /translation="MKSDVVFGLLDASWPVLLVGETGVIQMANKKAVETLGPTVETGS
                     TPLESLWRIENNIAPAEFFVRCEASPSTPASIRLKGAGGVDFWFQAWVSIFVDDGKKS
                     FILQLHPPENKPNGVEAGLAHKQKLDCALQLARTVSLDFNNALTSILGHTSLILSKLE
                     PDNPWRRSLMEVEKSAAKAAEIANDLGAFSRQEKEAKVQMSGNLNTLLQRNVEMFQSA
                     KLDKEVAWSLQLERKLFMAKFDEAKMQQALLKIMENAIESLGSANGRIILQTKNIELT
                     EPTQDRNAQLAAGSYVCAEIADNGCGIEPDVLPRIFEPFFTTKRGSKHRGLGLAWVYG
                     IVTNLGGVVAVSSQSNTGTSVRVYLPADKKIVRDHSTATISLNGSETILMVDDEDLLL
                     TMGQTILSTYGYHVLTANNGQKALEILSKNERPIDLLLTELVMPGMSGRELVEKVRQV
                     SPFTRILCSSGYVRPTSQEVEQTAYLQKPFTSQELLMKVKQALSAGEPAPVDS"
     gene            complement(2179..2574)
                     /locus_tag="VG754_03945"
     CDS             complement(2179..2574)
                     /locus_tag="VG754_03945"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWF18389.1"
                     /translation="MSKLDLMMAAMEARERAVAPYSKFKVGAALLTVEGEIITGANVE
                     SASYGLTCCAERVALFKALTEGHTRFAAIAVAARMKPVPMPCGACRQLLAEYAPKALV
                     WTADSRSPRKIREFTVAELLPAAFLEVPK"
     gene            complement(2567..3382)
                     /locus_tag="VG754_03950"
     CDS             complement(2567..3382)
                     /locus_tag="VG754_03950"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HWF18390.1"
                     /translation="MDPKLTARQIGKLTRLRPTLALVLGSGFQQAMARLEVETEIAYE
                     KLPGFPPVGVSGHAGKLLLGVFGRTPVLALSGRAHFYEGHSMEQVTFAIRVLAEFGVQ
                     DLLLTNAAGGINRKFRPGDFMALTDHINLMGTNPLRGAASSRWPRFVDMTQTYDAGLS
                     ALLHKAARSCKVKLKSGIYLAVSGPSYETPAEIEAFARLGADAVGMSTVPEAIVARQC
                     GLKVAGLSCITNIAAGRGVKTLSHAEVLQTAEKVKDTAGFLLQTFANEYGEGE"
BASE COUNT          796 a         1111 c          904 g          842 t
ORIGIN      
        1 cgatgaattt ttgcccggac aaccccttga gcttcacgct cttcatcttc gcgaacggat
       61 gttgcggatg gcagatgagc acgagcgggt ccttgcgcag agaaaccgtc tccagcttgc
      121 tttcgcgcat cggatacgca accaaaccca aatccacgac gttgctcagc acatcgtcat
      181 aaacttgatt cgcgcggcgg tattcgacgt gcacgttcac cgtcgggtag ctcttgagga
      241 attttttgat gtacggcgga aggtcgtgca gcccgatgct gtaaatcgtc gccacgcgga
      301 tggtcccgga aatgatgtcc ttgatctcct gcaatttgct gtggagcgaa tcgtagcatt
      361 ggatgatttg tttgctgaaa tcgtaaagca cctggccttc acgcgtgagc cggaatttct
      421 ttttgctgcg ttcgatcagg agcgatttga actgccgctc cagcgagctg atctgctggc
      481 tgaccgcgga ttgtgtgaca ttattgattt gcgcggcctt ggtgaaactc tcggtttcag
      541 ccaggtcgca aaagactttg agactttcta tttgcataag ttgtgattat tgaaccgtaa
      601 tccgcttcat gagtcaacag gcgcgggttc gccggcgctt aaagcctgct tgaccttcat
      661 aagtaattct tgacttgtga aaggtttttg caggtaagcc gtctgctcca cttcctgcga
      721 tgtgggccgc acgtacccgc tggaacaaag gatgcgcgtg aagggcgaaa cttgccggac
      781 tttttccacc aactcgcgtc cactcatgcc cggcatgacc aactccgtga gcaaaagatc
      841 aatcggccgt tcgtttttcg aaaggatttc cagcgctttc tggccgttgt tggcagtcaa
      901 cacgtggtaa ccataagttg ataaaatggt ctgccccatc gtgagaagga gatcttcgtc
      961 atccaccatc agaatggttt cgctgccatt gaggctgatc gtggcggtcg aatggtccct
     1021 cacgatcttt ttgtccgccg gcagatacac gcgcaccgaa gtgccggtat tggactggct
     1081 tgacaccgcg accacgccgc cgaggttcgt cacaatcccg taaacccacg ccagcccaag
     1141 cccgcgatgt ttgctcccgc gcttggtggt gaaaaatggc tcgaaaatcc gcggcaacac
     1201 gtccggttcg atgccgcaac cgttgtccgc gatttccgcg caaacgtacg agcccgccgc
     1261 cagttgcgcg ttgcggtcct gggtcggctc ggtcaattca atgtttttcg tctgcaaaat
     1321 gattcgtccg ttcgcgctgc caagagactc gatggcattt tccatgatct tcaacagcgc
     1381 ctgctgcatc ttcgcttcat cgaatttcgc catgaaaagt ttgcgttcca actgcagcga
     1441 ccacgccact tctttgtcca gcttggccga ttgaaacatt tcgacgttcc gctggagcaa
     1501 tgtgttcaag ttgcccgaca tctgcacctt ggcttctttt tcctgccggc tgaacgcgcc
     1561 gagatcattc gcgatttcag cggcttttgc ggccgatttt tccacttcca tcagcgaacg
     1621 acgccacgga ttgtcaggct caagcttgct caagatgagc gatgtgtggc cgaggatgct
     1681 cgtcagcgcg ttgttgaaat cgagcgaaac cgtccgcgcc aattgcaatg cgcaatccaa
     1741 tttttgcttg tgcgcaaggc cggcctccac cccgttgggc ttgttctccg gtggatgcaa
     1801 ttgcaaaata aaacttttct tcccgtcatc cacgaaaatt gaaacccacg cttgaaacca
     1861 aaaatccacg ccaccggcgc ccttcaagcg gatcgatgcc ggagtggaag gtgacgcctc
     1921 gcagcggacg aaaaattcgg cgggagcaat gttgttttct atgcgccaaa gcgattcgag
     1981 cggcgtcgaa ccggtttcca cagtggggcc gagcgtttcg acggcctttt tgttggccat
     2041 ctggatcacg cctgtttctc ccaccaacaa taccggccag ctcgcgtcca aaagcccgaa
     2101 aacaacatct gacttcattt acaaaaataa tttggccgct acatcctacc cgaaccggcc
     2161 caaatctcaa ccggtgattc atttcggcac ttccaggaag gcggctggca gcaattccgc
     2221 cacggtgaac tcgcgtatct tgcgcggcga acggctgtcc gccgtccaaa ccagggcttt
     2281 cggcgcgtac tccgccagca attgccggca cgcgccgcag ggcatcggga ccggtttcat
     2341 gcgcgccgcg accgcaatcg ccgcaaaacg cgtgtgtcct tccgtcaacg ccttgaacaa
     2401 cgccacacgc tccgcgcaac aagtcagccc gtaactggcg ctctcgacat tcgctccggt
     2461 gatgatttcg ccttcaaccg tcaacaacgc cgcgccgact ttaaacttcg aatacggcgc
     2521 gaccgcccgt tcccgtgcct ccatcgccgc catcatcaaa tctaatttac tcaccttccc
     2581 catattcgtt cgcaaaagtt tggagcaaga agcctgccgt atccttcacc ttttcagcgg
     2641 tttgcaaaac ttccgcatga gacaaagttt tgacgccgcg acccgccgca atattcgtaa
     2701 tgcacgacaa accagccact ttcaacccgc attgccgcgc cacaatcgcc tccgggaccg
     2761 tgctcatccc caccgcatcc gcgccaagac gcgcaaatgc ctcaatttca gccggtgtct
     2821 cataactagg cccgcttacc gcgagataaa ttcctgattt aagtttcacc ttgcagcttc
     2881 tcgccgcctt atgcagcaaa gcggacaaac cggcatcata agtctgcgtc atatccacaa
     2941 atcgcggcca ccttgacgac gccgcgccgc gcaacggatt ggttcccatc agattgatat
     3001 ggtccgtcag cgccatgaaa tcgcccggac gaaacttgcg attgatgccg ccggcggcgt
     3061 tcgtcagcag caaatcctgg acgccaaatt ccgccagcac gcggatggca aaggtcactt
     3121 gttccatcga atgaccttca taaaaatgcg cgcgcccgct caacgccagc accggcgtcc
     3181 ggccaaaaac ccccagcaac aattttcccg catggccgct cacacccacc ggcggaaagc
     3241 caggcagttt ctcgtaagca atctccgtct cgacctccag gcgcgccatc gcctgctgaa
     3301 aaccgctgcc caaaaccaag gccaacgtcg gccgcaagcg cgtcaatttg ccgatctgcc
     3361 gcgccgtcaa ttttgggtcc accgcatgct tcataacagt gccgtgaagc aaagcacttt
     3421 tcccgctcgc atcaaagggg attttgtttc tacgcaaagg cgcaagatct ggcccgccaa
     3481 gtccaaaaag acaaggtctt gcttgattgt tttcctgcga tgccgttata gacggggctg
     3541 tgaacgatcg ttacgaaatt gaatacggga cttcggctct ggatgacctt gacggtttgc
     3601 ccgaacgtca acgcgctcaa attcttcgca aaatcgaacg tctggagcat ggc
//
LOCUS       DASZMM010000161         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2818126, whole genome shotgun sequence.
ACCESSION   DASZMM010000161 DASZMM010000000
VERSION     DASZMM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2818126"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="VG754_03955"
     CDS             <1..1711
                     /locus_tag="VG754_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWF18391.1"
                     /translation="DATPGGVTIKASTQAGLFYGVQTLLELFPPQIFSINTLKNVAWN
                     IPYSVHIEDQPRFQWRGFMLDCARHFFSKDDVKRVLDEMALHKLNMFHWHLTDDQAWR
                     IEIKKYPKLTEVGAWRANAALVPYDNAGDQGNAHPAWSAAPASAFGADGRYGGFYTQD
                     DVREVVVYAAERHITVVPEIEMPGHSVAALAAYPELSCVDGHFSTDINAGVNHGVFCV
                     GNPKVIQFLDDVLTEVFQLFPAKYVHIGGDEVDRKAKSETWGKDPACLALMKEHSYTN
                     LDQLQGWFTGQMGKFISAHGKTLVGWSEITEAELPKDAVVMDWIGGAVQAATNGDDVV
                     MSPTRFSYFDYYQSQDHTTEPRAIGGFLPLEQVYSFEPIPAALPPEYDSHILGSQANL
                     WTEYIPSLAHAEYMMFPRLCALAEVAWSPKAARNWDDFSRRLRVQEVRLDELGVNHRR
                     QLSVKIGEWTPTQLTNAASSLLECDVTAQMKSPGQYRVTFQYAHGPGLVLNSVSLLED
                     GKEVATDVHTGFAARNPSKQVYVMDVPAVNPNAKYTLRASVSGQKSSGAILWAVESAD
                     KKP"
     gene            1964..2863
                     /locus_tag="VG754_03960"
     CDS             1964..2863
                     /locus_tag="VG754_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWF18392.1"
                     /translation="MTKPTLLVLAAGMGSRYGGLKQIDPVGPAGETIIDYSIFDAVRA
                     GFGKLVFVIRRDIEDAFREAIGARFEKRLPVEYVFQELDKLPPGFTVLPNRKKPWGTG
                     QAILMAADVIREPFAAINADDFYGATSFRVLAEHLRSGSADYAMVGFVLRNTLSEFGS
                     VARGACQVGPDGYLQAVTELTKIEKDGAGAKYTDATGAAQRLSGDEPVSMNMWGFTPS
                     LFPHLQEQLIQFLKAHGQEEKSELYIPSVVNELVRAGQERCQVLRTTDSWFGVTYRED
                     RPFVVEGIRALIARGDYPQALWG"
     gene            complement(2919..>3359)
                     /locus_tag="VG754_03965"
     CDS             complement(2919..>3359)
                     /locus_tag="VG754_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18393.1"
                     /translation="LLSADEDCMSKLALLCADRLVLMTIITLSVSRPCYRALIFRSVL
                     AAGAHLRAAHRFPAGCFQLPAADPTSAGRPIHRRPVERLEKEGRKNRKINLKKKEEKA
                     KVPIGLAANNAIAGEKFQIPSSKLHGSSKSQAPVQRIRQRFFGT"
BASE COUNT          741 a          931 c          949 g          738 t
ORIGIN      
        1 ggacgcgacg ccgggtggag tgacaatcaa agcctccacc caagccggtt tgttttacgg
       61 cgtgcaaacc ttgctggaat tatttccgcc gcaaattttt tccatcaaca cgctaaaaaa
      121 cgtcgcctgg aatattccct attcggtgca tatcgaggac cagccgcgct tccaatggcg
      181 cggttttatg ctcgattgcg cccgccactt tttctccaaa gacgatgtca aacgcgtact
      241 ggatgaaatg gcgctgcata aattgaacat gttccattgg cacctgaccg acgaccaggc
      301 ctggcggatc gaaatcaaaa aatatcccaa actcaccgag gtcggggcgt ggcgcgcgaa
      361 cgctgctttg gtgccgtacg acaacgcggg tgaccaaggc aatgctcatc ccgcctggtc
      421 cgccgcaccg gcgtctgcct ttggcgcgga tggccgttac ggcggctttt acacgcagga
      481 cgacgtccgc gaagtcgtcg tctacgccgc ggaaaggcac atcaccgtcg ttcccgaaat
      541 cgaaatgccc ggccattcgg tggcggcgct ggcggcgtat ccggaactca gttgtgttga
      601 cggccatttc agcaccgaca ttaacgccgg cgtcaatcac ggcgttttct gcgtcggcaa
      661 cccgaaagtg attcagtttt tggacgatgt gttgacggaa gtttttcaac tgttcccggc
      721 gaaatacgtt cacatcggcg gtgatgaagt ggaccgcaag gccaagagcg aaacctgggg
      781 aaaagatccc gcctgtctcg cgctgatgaa ggagcacagc tatacgaatc tcgatcaatt
      841 gcaggggtgg ttcaccgggc agatgggcaa attcatcagc gctcacggaa aaaccctcgt
      901 gggctggagc gaaattaccg aggccgagtt gcccaaagac gccgtggtca tggactggat
      961 cggcggcgcc gtccaggccg cgaccaacgg cgacgatgtc gtgatgagtc cgacccggtt
     1021 ttcttatttc gattactacc aatcgcagga tcatacgacc gagccgcgcg ccatcggcgg
     1081 ttttctgccg ctcgaacagg tttattcctt tgaaccgatt cccgccgcgc tgccaccgga
     1141 atacgattcc catattcttg gcagtcaggc caatttgtgg accgaataca ttccctcact
     1201 cgcgcacgcc gaatacatga tgttcccgcg cctttgcgcg ctggcggagg tggcctggtc
     1261 gccgaaggcc gcgcgcaact gggacgactt cagccgccgc ctgcgcgtcc aagaggtccg
     1321 gttggatgag cttggcgtca atcaccgccg ccaactctcc gtaaaaatcg gcgaatggac
     1381 acccacgcaa ttgacaaatg cagcgagctc tcttctggaa tgcgacgtga ccgcgcagat
     1441 gaagagcccg ggccaatatc gggtgacgtt tcagtacgcg cacggccccg gattggttct
     1501 taattcggtg tcgttgttgg aggacggcaa ggaagttgcg acggatgtgc ataccggttt
     1561 cgcggcgcgc aacccgagca agcaggttta tgtgatggat gttcctgcgg tgaatccgaa
     1621 cgcgaagtac accttgcgag catccgtttc cggtcagaaa tcatcgggtg caattttatg
     1681 ggcggtggaa tcggcggaca aaaagccgtg aacaggcgga aatttacgcc gcggccagtt
     1741 tatgcgctga atttcaacgc aaaggcgcaa agcagccaag acgcaaagcg ggaacggcaa
     1801 aaaccagaat gttgatctga agattcccct aactaaacag taatccaaaa gatgccatcg
     1861 aaaacatttc aagtttttgc gccttcgtgt ctttgcgcct ttgcgttaaa aattttctga
     1921 atcggtacgg attcggtccg cgtttctata aatagaatta acaatgacca aaccaacttt
     1981 gcttgtcctg gccgccggca tgggcagccg ctacggcggc ctcaaacaaa tcgaccccgt
     2041 cggccccgcc ggcgaaacca tcattgatta ttccatcttt gacgctgtgc gcgccgggtt
     2101 tggaaaactg gtctttgtca tccgccgcga catcgaggac gcctttcgcg aggcgattgg
     2161 ggcgcgtttc gaaaaacggc tgccggtgga atacgttttt caggaattgg acaaattgcc
     2221 gcccggtttt accgtcctgc cgaaccgcaa aaaaccgtgg ggcaccggcc aggcaattct
     2281 gatggccgcc gatgtcatcc gcgagccctt cgccgccatc aatgccgatg atttttacgg
     2341 agcgacgtcg tttcgcgtgc tggcggagca tttgcgctcc ggctcggcgg attacgccat
     2401 ggtcggtttt gtattgcgaa acacgctctc tgagttcggc agcgtggcgc gcggcgcgtg
     2461 ccaggttggg ccggatggtt atttgcaagc tgtcaccgaa ctgaccaaaa tcgaaaaaga
     2521 tggcgccggc gcgaaataca ccgatgccac aggagccgcg cagcgcctca gtggcgatga
     2581 gccggtctcg atgaacatgt ggggtttcac gccaagtctg tttccgcatt tgcaggagca
     2641 attgatccag ttcctgaaag cgcacggcca ggaggaaaaa agcgaattgt atattccgtc
     2701 cgtcgtgaac gaactcgttc gcgccggcca ggagcgctgc caagtattgc ggacaacgga
     2761 ttcgtggttt ggtgtgacct atcgtgaaga ccggcctttt gtggtggaag ggatccgtgc
     2821 gctgattgcg cgcggcgatt atccgcaggc gctttggggc tagcgcgcgg agaaaatccc
     2881 caaggtccag catccaagcg ccagagaagc tccgaatcct aagttccaaa aaatcgctgg
     2941 cggatgcgct ggactggagc ttgggatttg gagcttccat gaagcttgga acttgggatt
     3001 tggaactttt cccctgctat cgcgttattc gctgcaaggc ctatcggcac tttagctttt
     3061 tcttcctttt tctttaaatt tattttccga tttttccttc cttccttctc aagtctttcc
     3121 accggtctcc gatgaatggg gcgtcccgca gaagtgggat cggcagccgg tagctgaaaa
     3181 cagccggcgg gaaaacggtg cgctgccctt aaatgcgcgc ccgcagcaag gacgctgcga
     3241 aaaatcaatg ccctgtaaca ggggcgactc acggaaagag ttattatggt catcaatacc
     3301 aacctgtcgg cgcagagcag cgcaagcttg ctcatgcaat cctcgtctgc actcagcaa
//
LOCUS       DASZMM010000162         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2818892, whole genome shotgun sequence.
ACCESSION   DASZMM010000162 DASZMM010000000
VERSION     DASZMM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2818892"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..301
                     /locus_tag="VG754_03970"
     CDS             59..301
                     /locus_tag="VG754_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWF18394.1"
                     /translation="MQATIQQWGNSLALRIPKAFAQQTKVKKGSRVNLTLKNGRVIME
                     PIKRQKYTLQELVSRITSQNRHSETSWGMPQGKEVW"
     gene            303..650
                     /gene="mazF"
                     /locus_tag="VG754_03975"
     CDS             303..650
                     /gene="mazF"
                     /locus_tag="VG754_03975"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HWF18395.1"
                     /translation="MAGYVPDRGDIVWINFDPQAGHEQSGRRPAVVLSPRSYNRPAGL
                     ALLCPVTNRSKGYPFEVTLPEDSTVTGVVLTDQVRSLDWRSRQVAFIGALNEQILIEI
                     IERLRPLLGFDNE"
     gene            656..1531
                     /gene="argB"
                     /locus_tag="VG754_03980"
     CDS             656..1531
                     /gene="argB"
                     /locus_tag="VG754_03980"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWF18396.1"
                     /translation="MQNLISKADVLLEALPYMQRFRGQTFVIKYGGSFMDSPDAAVRQ
                     GVARDVVFLATAGINPVVVHGGGKAITRAMDAAGLKANFIQGMRVTDDATVKVVDQVL
                     SREINPEVVASIHSLQGTARGFAGTEIFTCRKLWLNGASGEKIDIGFVGEVTGVNTAP
                     LLECLAQGMIPVVSPTARDEHGQIYNCNADVAAAQAAIALKAKRLVFMSDVPGLMRNP
                     TDEATLISRLRIGEVDELKRAGIVDKGMIPKVDSAVAAIKAGVEKVSFVDGRMPHSVL
                     LEIFTDKGVGTEVTL"
     gene            1542..2135
                     /locus_tag="VG754_03985"
     CDS             1542..2135
                     /locus_tag="VG754_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18397.1"
                     /translation="MGQTCEGATVTDPDAYLLRENTGTTGLELTARAAAAALAQHHIP
                     HLIVGGFAVQEHGYPRLTIDLDIIVPDVLETAEFLTADLSGPFARVPGQQDRVKDKRN
                     GVFIDLLPAGHVAKQGCKVPFPLPEKASDKPQIVTLENLISLKLDSWSNSPLRRLRDK
                     SDAIELITRRKLPRDLGVAEAVRSHYLEIWDGLQAER"
     gene            2196..3482
                     /locus_tag="VG754_03990"
     CDS             2196..3482
                     /locus_tag="VG754_03990"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HWF18398.1"
                     /translation="MKEIVPSPPPMIHNEAAEIQSLFQKNVLPSYGRFDVVLSHGAGS
                     QLWDVAGKRYLDLGGGIAVCSLGHAPPAITDALIEQSRKLVHVSNFYYHAPQGRLAQA
                     LVKLIGPGKCFFSNSGVEANEGLFKLARKFGHDEGSFEIITTVNSFHGRTLAAIAATG
                     QEKVKKGFEPAVPGFRHVPYNDLAAMRAAISPATAAIMIEGIQGEGGVTPATAEYLLG
                     LRQLCDEKKLLLFMDSVQCGHFRTGRFQSFQRTLEGVPGGDKFLPDGISMAKSLGGGF
                     PMGAFWVRQPYADLLSAGSHGTTFGGSPLACAVALKILSVIEQEKLADNAREVGEHLK
                     AGLQDLAHKYPGVVKNVRGLGMMIGIELAPNIPAFSGEGKTPTVRFTNLLHAAGLLAI
                     PAGTQIIRLLPALNLRRGEADEGLRIIGSVVEKLAS"
BASE COUNT          823 a          940 c         1031 g          784 t
ORIGIN      
        1 tgggagggta aggcttgcaa ttaactttct ttgtatgtag tctttttgta gatacattat
       61 gcaagcaaca atccaacaat ggggaaacag cctggcactg cgcattccca aggcatttgc
      121 ccaacaaacg aaagtgaaaa agggcagccg agtgaacctt accctgaaaa acggccgtgt
      181 gatcatggag ccaataaaac ggcaaaaata cacattacag gaacttgttt ccagaatcac
      241 atcgcaaaac cgccattcgg aaacaagctg gggcatgccg cagggaaagg aagtctggta
      301 aaatggcagg ttacgttccc gatagaggcg acatcgtgtg gatcaatttc gatccacaag
      361 ccggacatga acaaagcggt cgccgccccg ccgtcgtact gtctccgcgc agctacaatc
      421 ggcctgctgg tcttgccttg ttatgtcctg tcaccaaccg cagcaaaggc tatccatttg
      481 aggtgacgtt gcctgaagat tcaacggtaa caggtgtcgt tttgacggat caggtccgca
      541 gtctggattg gagatcgcga caagtggctt ttataggggc gttgaatgag caaatcttga
      601 ttgaaatcat cgaacgcctg cgtccattgc ttggatttga caacgaatag atccaatgca
      661 aaatctcatt tccaaagccg acgtgttgct cgaagcgctg ccctacatgc agcggtttcg
      721 cgggcagaca ttcgtcatca aatacggcgg gagtttcatg gattcaccgg acgcggcggt
      781 acgtcagggc gtggcgcgcg atgtagtttt tctcgcaaca gcaggcatca atcccgtggt
      841 cgttcacggc ggcggcaaag ccatcactcg cgcaatggat gccgctggtc tcaaggccaa
      901 tttcatccag gggatgcgtg tcactgatga tgccactgtg aaagtcgtgg accaggtgct
      961 ctcgcgcgaa atcaatcctg aagttgttgc cagcattcat tcattgcaag gcaccgcgcg
     1021 tggttttgcg ggcacagaaa ttttcacctg tcgcaagctt tggctgaacg gcgcgtcggg
     1081 cgaaaaaatt gacatcgggt ttgtcggcga agtcacgggc gtaaacacag cgcctttgct
     1141 cgagtgtctt gcgcaaggaa tgattccggt cgtcagcccg actgcgcgcg acgagcatgg
     1201 gcagatttat aattgcaatg ccgatgtcgc agccgcgcag gcagccatcg cgttgaaggc
     1261 gaagcggctc gttttcatga gcgatgtgcc cggcctgatg cgcaatccca cggatgaagc
     1321 gacgttgatt tcacgattgc gcatcggcga agtggacgaa ctgaaacgtg caggcatcgt
     1381 ggacaaggga atgattccca aggtggacag cgcggtcgcc gccatcaaag ccggcgtgga
     1441 aaaagtttcg ttcgtggacg gtcgtatgcc acattccgtg ttactggaaa ttttcaccga
     1501 caagggcgtg ggaactgaag tcacgctctg acattgaatc gatggggcaa acatgcgaag
     1561 gtgcgacggt gaccgaccct gacgcctatc tgcttcgtga gaacaccggg acgaccggat
     1621 tggagttgac tgcccgcgcg gcagccgcgg cgctcgcgca acatcacatt ccgcatctga
     1681 tcgtgggcgg cttcgcggtg caggaacatg gttatccccg cctgacaatc gatttggaca
     1741 tcattgtccc agatgtgctc gaaacagcgg aatttttgac ggcggatttg tccggtccgt
     1801 tcgcgcgcgt tcccggccag caggacaggg tgaaagacaa gcgtaacggc gtcttcattg
     1861 atttgctccc cgcaggccat gtggccaaac aaggctgcaa ggtgcccttt ccgctgcctg
     1921 aaaaagcctc cgacaagccg cagattgtga ctctggaaaa tttaatttca cttaagcttg
     1981 atagctggag taactcgccg ttgcgtcggt tgcgggacaa gtcggatgcc attgaactga
     2041 tcacccgtcg caaactcccg cgcgacttgg gcgtcgccga agccgtccgc agtcattatc
     2101 tcgaaatctg ggacggactg caggcggaac gttgattctt cttttctccc ggaattttcc
     2161 tgctaaagat ttccctttcg gttgatgacc taaacatgaa agaaattgtt ccatcaccgc
     2221 cgccgatgat tcacaacgaa gcggccgaga tacaaagttt gttccagaaa aatgtcttgc
     2281 cttcatacgg aaggttcgat gtcgtgctca gccacggcgc gggcagccag ctttgggacg
     2341 tcgccggcaa acgctatctc gatcttggcg gcggcatcgc ggtctgctcg ctcggccatg
     2401 caccgccggc gataacggat gcattgatcg agcaatcacg caagctggtg catgtttcca
     2461 atttttatta ccacgcgccg cagggacgtt tggcccaggc attggtaaaa ttaatcgggc
     2521 ccggcaaatg ctttttttcc aacagcggcg tggaggccaa cgaaggtttg ttcaagctgg
     2581 cgcgaaaatt cggccacgac gaaggcagtt tcgaaatcat caccaccgtg aattctttcc
     2641 atggccgcac actcgcggcc atagccgcga cagggcagga aaaggtcaag aaaggttttg
     2701 aaccggcagt gccgggtttt cgtcacgtcc cctacaacga tttggcggca atgcgcgcgg
     2761 cgatttcacc cgcaacggcc gccatcatga ttgaaggcat ccagggcgaa ggcggcgtga
     2821 ctccagcgac ggcagaatat ctgctcggtt tgcggcaatt gtgcgatgaa aagaaattac
     2881 tgctcttcat ggacagcgtg caatgcgggc atttccgcac cggacggttt caaagtttcc
     2941 aacgaacgct ggaaggcgtt cccggcggcg ataaattttt gccggacgga atctcgatgg
     3001 caaaatcgct cggcggcggt tttccgatgg gcgcattttg ggtgcggcag ccttatgcgg
     3061 atttgttgag cgcgggttcg catgggacga cgtttggcgg ttcgccattg gcctgcgctg
     3121 tcgctttgaa aatcctttcc gtcatcgagc aggaaaaact cgccgacaac gcgcgcgagg
     3181 ttggcgagca tttgaaggcc ggtttgcagg acttggccca caaatatccg ggcgtcgtca
     3241 agaatgttcg cggcttggga atgatgatcg gcatcgagct ggctccaaac atcccggcat
     3301 tctccggcga aggcaaaact cccaccgtgc ggttcacgaa tcttttgcac gcggcgggtc
     3361 tgctggcgat cccggctggc acccagatca tccggctgct gcccgcgttg aacctgcgcc
     3421 gcggcgaagc agacgaagga ttgcgcatca tcggttcggt ggtggaaaaa ctggcctctt
     3481 gaatccatga aacatttgtt gagcatcgag aaactgacgg cggctgagat ggaacaaatg
     3541 cttgccgcgt cagggccgtt gaaaaaagaa cggggcca
//
LOCUS       DASZMM010000163         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1167204, whole genome shotgun sequence.
ACCESSION   DASZMM010000163 DASZMM010000000
VERSION     DASZMM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167204"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VG754_03995"
     CDS             <1..1252
                     /locus_tag="VG754_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18399.1"
                     /translation="AVRTVTEVPLRLRVLNALTAYAHYAVKTLWPSHLSIYYLLPEKI
                     PVAPAVCAGIAMLAVTYLTFRWRLRWPWLLVGWLWFVVTLVPVIGLIQVGSQAMADRY
                     TYIPLIGLFIAVAWSFERLFVFAPGNRTSFVVMGSIVLLLCIALTKIQLGFWRDNVTL
                     FQQAADVSPDNYFAQYELGFALAQSGDNNAAIAHHETALRLNPLFEPAHYQIGIELTD
                     AGKLDDAAFHFGEALKRDPTSQELHNNLGVVFARQKKLDAAIEQFKETIQINPGYLKP
                     YLNCALAQQRLGQFGAAFANFTKARELKPGSLDVMNRFACFLATCPDAKWRDPAAALK
                     LSETVNDLTHREDPAYLSTLALANAAAGNFTNAVAVAELAMTKGNEQHLTILTKKIES
                     DLASYRLGKNPPVNWDSPSSEASH"
     gene            complement(1249..1662)
                     /locus_tag="VG754_04000"
     CDS             complement(1249..1662)
                     /locus_tag="VG754_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18400.1"
                     /translation="MKKLRPWLVLLLVFCAGTMFGIVATRIVTRRVIHRMITHPDFVR
                     ERIQRELVRKLKLTPGQEIQVEAVLTDSQKELRDLRQEFQPRFVFIIADAQNRISAVL
                     TPDQRERFEKLKEQNRHYFPSGPVSADKPRATGTN"
     gene            complement(1669..1968)
                     /locus_tag="VG754_04005"
     CDS             complement(1669..1968)
                     /locus_tag="VG754_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18401.1"
                     /translation="MNDRKLKKLFELGRDEVPPRAPEGFEERVSRALRSERPNAPVSF
                     WEQIEALFPRVAIAAVLVMGCCLAAELSFSAAHPAGLSADVSELSDQWLFAGNND"
     gene            complement(1965..2588)
                     /locus_tag="VG754_04010"
     CDS             complement(1965..2588)
                     /locus_tag="VG754_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF18402.1"
                     /translation="MAPSWDLADAWRNSKFAACCSMVNDDAELLERIRKGAPDDFAEI
                     IRRHQDQVFAILHRYERDVQKVEDLAQDTFVKAWRHLDRFDGRAPFHHWLAKIASRVA
                     LDHLRRQKRIRSEVALPELGDDVLDWLRDPGEEAQLEAQQAAELLDIAMRELAPKDRQ
                     VLTLLEIEGHSVKKICELTGFSNAVVRVRAFRARARLKKILMRMENL"
     gene            complement(2548..3027)
                     /locus_tag="VG754_04015"
     CDS             complement(2548..3027)
                     /locus_tag="VG754_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18403.1"
                     /translation="MKISKWCAVTMVAALAAGGLSLTMAHARAQDAGGHVVRGRFLAK
                     AKEKLGLSDEQVAQIKAVLGADKDKLTGELTAFHEARIALRETIQKPGVSDDDVRAAS
                     AKVAAAEADLAVERSHLYGKISPVLSADQLAKVNEFQERVDDFVDGAIVGFGRRLAQ"
BASE COUNT          685 a         1005 c          800 g          731 t
ORIGIN      
        1 cgcagtcagg accgtcacag aagtgccgct acgtttgcgc gtcctcaatg cgctcaccgc
       61 gtatgcgcat tatgccgtca aaactctctg gccatcgcat ctttcgattt actatttgct
      121 tccagaaaaa attccggttg ctccggcagt ctgcgcgggg attgcaatgc tggccgtgac
      181 ttatcttacg ttccgatggc gtttgcgatg gccgtggctg ctggtcggat ggctctggtt
      241 cgtagtaacg ctcgtgccgg tgattggact catccaagtc gggagccagg caatggccga
      301 tcgttacacc tacataccgc tcatcgggtt gttcatcgcg gtggcgtgga gctttgagcg
      361 cctgttcgtt ttcgcgccgg ggaaccgcac ttcctttgtc gtgatgggtt ccattgtgtt
      421 gctcctgtgc attgcactga cgaaaatcca gcttggcttc tggcgcgaca acgtcaccct
      481 cttccaacaa gccgccgatg tttctcccga caactacttc gcccaatacg aactcggctt
      541 cgcgctcgcc caatccggcg acaacaatgc cgccatcgcg caccacgaga ccgccctgcg
      601 tctcaatcct ctttttgaac ccgcgcatta tcagattggc attgaactca ccgacgccgg
      661 aaaattggac gatgccgcgt tccattttgg cgaagccttg aagcgcgatc ccaccagcca
      721 ggaattgcac aataatctcg gcgtcgtttt tgcgcggcaa aaaaaattgg acgcggccat
      781 cgagcagttc aaggaaacca tccagatcaa tcccggttac ctcaagcctt atctcaactg
      841 cgcgctcgct caacaacggc ttgggcaatt cggcgctgcg tttgccaatt tcaccaaggc
      901 ccgcgaattg aaacccggtt cactggatgt gatgaaccgt tttgcctgct ttctcgcaac
      961 gtgccccgac gcaaaatggc gcgacccggc ggccgcgcta aaactttccg aaaccgtgaa
     1021 cgacctcacc caccgtgaag acccggccta tctttcgaca cttgccctcg cgaacgcagc
     1081 cgccggaaat ttcaccaacg ccgtcgccgt tgccgaactc gcgatgacca aaggcaacga
     1141 acagcatctc accattttga cgaaaaaaat tgaaagcgac cttgcctcct accgtcttgg
     1201 caaaaatcct cccgtcaact gggattcgcc ttcgtctgaa gcctcacact aattcgtgcc
     1261 cgttgctcgc ggtttgtcag cgctcactgg gccagatgga aaatagtgac ggttttgctc
     1321 tttgagcttt tcaaatcttt cgcgctggtc gggtgtcagc acggcggaaa tccggttttg
     1381 cgcatcggca atgatgaaaa cgaaccgcgg ttgaaattcc tggcgcaggt cgcgcaattc
     1441 cttttgggaa tcggtcagca ctgcttcaac ttggatttcc tggccgggtg tcagtttcaa
     1501 ttttctgacc aattcccgct ggatgcgttc gcgcacaaaa tcgggatggg tgatcatccg
     1561 gtggatgacc cggcgcgtca cgatccgcgt ggcgacgatg ccgaacatcg ttccggcgca
     1621 aaacaccaat agcaacacca gccatggcct gagtttcttc ataggtctct aatcattatt
     1681 cccggcaaac agccattggt ccgaaagctc gctcacatcc gccgaaagcc ctgccggatg
     1741 cgccgcagaa aaactcaatt cggccgccag gcagcagccc atcaccaaaa ctgcggcaat
     1801 ggcgacgcgc ggaaacagcg cctcaatctg ctcccaaaat gaaaccggcg cgttgggacg
     1861 ctccgaacgc aacgcccgcg agacgcgttc ttcaaatccc tccggcgctc gcggcgggac
     1921 ttcgtcccgg cccaattcga acagtttttt aagtttgcga tcgttcataa attctccatc
     1981 cgcatcaaaa ttttcttcaa acgcgcccgc gcccgaaaag cccgcacccg caccaccgca
     2041 ttcgaaaatc cggtcagttc gcagattttc ttcacgctat ggccctcgat ttccagcaac
     2101 gtaagcacct gccggtcttt cggcgcaagt tcccgcatgg cgatgtcgag caattccgct
     2161 gcctgctgtg cttcaagctg cgcttcttcg cccggatcgc gcaaccaatc cagcacgtcg
     2221 tcgccaagtt caggcaatgc gacctcggaa cgaattcgtt tttgccgtcg cagatggtcc
     2281 agcgcgacac gcgaagcaat ttttgcgagc caatgatgaa acggcgcgcg gccgtcgaac
     2341 cggtcgagat gacgccacgc cttgacgaac gtgtcctggg ccaggtcctc gactttttga
     2401 acgtcccgtt cataccggtg gagaatcgca aagacctggt cctggtgccg ccggatgatt
     2461 tccgcgaaat cgtccggcgc tccctttcgg attcgctcca gcaattcggc atcgtcgttc
     2521 accattgagc agcacgcggc gaatttacta ttgcgccagg cgtctgccaa atcccacgat
     2581 ggcgccatcg acaaaatcat ccacccgctc ctgaaactca ttcaccttgg caagttgatc
     2641 agcggacagg acggggctga ttttgccata aagatgagac cgttcaacgg ccaagtcagc
     2701 ctccgctgcc gcgactttgg cggatgccgc gcgaacgtca tcgtcggaaa cacctggttt
     2761 ctggattgtt tcgcgcaacg cgatgcgcgc ttcatgaaac gcagttaatt caccggtaag
     2821 cttgtctttg tccgcgccca ataccgcctt gatctgtgcg acctgttcat cggaaagacc
     2881 gagcttctcc ttcgcctttg ccaggaaacg tccccgaaca acatggccgc cagcatcctg
     2941 cgcgcgggca tgggccatcg ttaaactcaa accgccagcg gccagcgccg cgaccatcgt
     3001 aactgcacac catttcgaga ttttcatacg taatatctct acgcagggcg gcaaaggaat
     3061 gttacagctt acggcggttt aaatgattga aaggaacccc gctcttttct aaggtctttt
     3121 ccatgagccc attttatcgc tgtctccttt cagccctgct cgcgttgggt attgcgcaag
     3181 cgtccatggc cgcggacaaa acctacacca tcgccgtgat t
//
LOCUS       DASZMM010000164         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_340122, whole genome shotgun sequence.
ACCESSION   DASZMM010000164 DASZMM010000000
VERSION     DASZMM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340122"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..982)
                     /locus_tag="VG754_04020"
     CDS             complement(167..982)
                     /locus_tag="VG754_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF18404.1"
                     /translation="MESENLELRIYGDPALPTLVYLPGLHGDWTLVASFRAALKDRVH
                     FVEMTYPRSLTWRINDYAEAIESALLEHGITRGWLLAESFGSQLAWPLLERHLADKDQ
                     PEGARSKAFHVQGLILAGGFVKHPWPWGPGALRWIGRMTPMWLYRLELKIYSWYSRFR
                     HRKAPEVLDTIAEFVSRRTALDRQAMQHRLTLLDGYDPRDIARRACVPIHYLAGFVDP
                     LVPWVLVRWWLRKNCPTYRGGKTFWLADHNVLATSPARAANLVMKWMREGAAE"
     gene            complement(985..>1713)
                     /locus_tag="VG754_04025"
     CDS             complement(985..>1713)
                     /locus_tag="VG754_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF18405.1"
                     /translation="IISFCVGLFIWLISQNVGAETVACSSTGDFVVLLHGMGRTSLSM
                     KRMEHFLTEHGYNVINVTYRSRRFSVDELAEKHLKHLLESRVIDDGRKVHFVTHSLGG
                     IIVRQYLSKHKIKNLGRVVMLAPPNHGSEIIDRLRANSITRNFLGASARELGTGADGL
                     PTKLGPVQFPCGVIAGDRSLNPLLSSLLPCPNDGKVTVASARVDGMQGFLVVHGTHTW
                     LMCKQSVMNQTLSFLQSGYFSEPH"
BASE COUNT          406 a          503 c          446 g          358 t
ORIGIN      
        1 aatgtgggaa taccggttgc gtccgaatta gtcgtgcgtg ttttggttct ggtgcgttgc
       61 tgcggctcac cgagccgcgc tccgttttag ccggtgaacg tcccggcaag ctcaacccat
      121 ctatccggca tgaccgacaa atcccgacag cgccaagacg ggcctgtcac tccgccgccc
      181 cttcccgcat ccacttcatc acgagattcg cggcgcgggc aggggatgtg gcaagcacgt
      241 tatggtcggc gagccagaat gttttaccac cgcgataggt tggacaattt tttctcagcc
      301 accagcgcac caagacccat ggcacaagcg gatccacaaa accagccaga taatgaattg
      361 gcacgcaagc ccgccgcgca atgtcgcgcg gatcgtatcc gtccagcaac gtcagccgat
      421 gttgcatcgc ctgccggtcc agcgctgtcc ggcggctcac gaattctgcg atcgtgtcca
      481 aaacctcggg cgctttccga tggcgaaagc gcgaatacca cgaataaatt ttcaattcca
      541 accggtacag ccacatcggc gtcatccggc cgatccatcg caatgctccc ggtccccacg
      601 gccatggatg tttcacaaag ccgcccgcca gaatcaatcc ttgaacgtga aatgctttcg
      661 agcgcgcacc ttcaggctgg tccttgtccg ccagatgtct ttccaaaagc ggccaggcca
      721 gttgcgaacc gaacgattct gcgagcagcc agccgcgcgt gatgccgtgt tccaacaacg
      781 cgctttcgat ggcctccgcg taatcattga tgcgccacgt gagcgaccgt ggataagtca
      841 tttctacaaa atgcacccgg tccttcaatg ctgcgcgaaa actggcaacc agcgtccaat
      901 caccatgcaa gcccggcagg taaaccagcg ttggcaaagc gggatcgccg tagatgcgaa
      961 gttcaagatt ttcggactcc attcttaatg cggctcgctg aaatatcctg attgaaggaa
     1021 agaaagggtc tggttcatta ccgactgctt gcacatcagc caagtatgcg tgccgtgcac
     1081 cacaaggaag ccttgcattc catctacacg ggcgcttgcc accgttactt ttccatcatt
     1141 tggacagggg agcaacgagg acaagagagg gttcagcgag cggtctcctg caatgacgcc
     1201 acaggggaat tgaacagggc caagtttcgt cggcaaacca tccgcacccg tcccgagttc
     1261 ccgcgcggag gcgccaagaa aattgcgcgt gatggaattg gcccgaaggc ggtctatgat
     1321 ttcgctgccg tgattcggcg gcgcaagcat cacaacgcga ccaagattct tgattttgtg
     1381 tttcgacaaa tattgccgaa caatgatgcc gcccaatgaa tgcgtgacga aatgcacctt
     1441 tcgcccgtcg tcaatgaccc tgctttcgag caaatgtttc agatgttttt ccgcaagttc
     1501 gtccacggaa aagcggcgcg agcggtacgt gacattgatc acgttatagc cgtgttcggt
     1561 gagaaaatgc tccatccgtt tcatggagag tgaagtccgc cccattccat gaagtagaac
     1621 aacaaaatct ccggttgaac tgcaagcgac agtttccgcc ccgacgtttt gcgaaataag
     1681 ccagatgaaa aggcccacgc aaaaactgat gat
//
LOCUS       DASZMM010000165         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1446866, whole genome shotgun sequence.
ACCESSION   DASZMM010000165 DASZMM010000000
VERSION     DASZMM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446866"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VG754_04030"
     CDS             <1..538
                     /locus_tag="VG754_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWF18406.1"
                     /translation="SGYALLGLLANNGQGVSSLIFYMATYGLTVVGAFAVVGVVEDNA
                     GDAGISNFAGLGRREPMVAFCMMVFMLSLAGIPPLAGFFGKFYLFVAVAGGSENLGLL
                     WLVIFAIAMSAVSLYYYLQVLKQIYVAPASPERQEMTVPISTRVVLVALAVGVVALGC
                     APNLLLGRLLAAIPSFAL"
     gene            621..1547
                     /locus_tag="VG754_04035"
     CDS             621..1547
                     /locus_tag="VG754_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18407.1"
                     /translation="MPGKRSIRSSTPLAAFTLLELLVVIAIIGILASLLLPALSRGKQ
                     KAQGIQCLNNGKQLMLCMTMYTGDYNDFFPPNPDDGNTITGHNWCGGQAGIGGADEFN
                     PDILKDPDRSLMAPYLKGNITVFHCPADKRFGAYQGSDPSFVGGSVPAARTFSMSQAV
                     GTICPGFDSGKQTHSGAPTLPVNGPWLNDQHNHIRDTPWITYGKMSTIRAPGPSKLWV
                     LVDEDVQGLNDAAFAFGMEKYQWRDAPGTYHNGGCGFAFADGHSETHKWFSSAEKDAG
                     SAITEESDQQDWLWVRERTSANINGAMPAPVP"
BASE COUNT          327 a          404 c          498 g          376 t
ORIGIN      
        1 ttccggttat gcgctgctcg gtttgctcgc gaacaacgga caaggcgtca gttcattgat
       61 tttttacatg gcgacatacg ggttgacggt ggtgggcgcg tttgcggtgg ttggagtggt
      121 tgaggacaat gccggggacg caggaatatc caattttgcc gggctgggcc ggcgcgaacc
      181 gatggtggcg ttttgcatga tggtcttcat gttgtcgctg gcgggaattc cgccgttggc
      241 tggattcttc ggaaaatttt atttgttcgt cgcggttgcg ggaggttcgg agaacttggg
      301 attgctgtgg ctggtgattt ttgcgatcgc gatgagcgcg gtttcgcttt attattatct
      361 ccaggtgttg aagcagatct atgtcgcgcc agcgtcgccg gagcggcagg aaatgactgt
      421 tccaatttcc acgcgggttg tgctggtcgc attggcggtc ggcgtggtgg cgctgggttg
      481 cgcgcccaat ttgctcttgg gccgattgct ggcggcgatc ccgtcattcg cgttgtgacc
      541 gcggcaagtc gctcggatta aagttgaccg tcggcaaaat ccggacaaga gtcgcgtcaa
      601 gaaaaggaaa aacacgctgt atgccgggaa agcgttcgat tcgttcaagc acacctttag
      661 cagctttcac actgctcgaa ttgcttgtgg tcatcgccat catcggcatc ctagcctcgt
      721 tgttgttgcc ggctctttcg cgcggcaagc aaaaagccca gggaatccaa tgcctcaaca
      781 atggcaaaca actgatgctc tgcatgacga tgtacacagg cgattacaac gatttcttcc
      841 cgcccaatcc ggacgacggc aacacgatca cgggacataa ttggtgcgga ggtcaggcgg
      901 gcattggagg agcggacgaa tttaatccgg acattctcaa ggaccccgac cgcagcctga
      961 tggccccgta cctgaaagga aatatcacgg tgtttcattg cccggcggac aaacggttcg
     1021 gcgcttatca aggttcggat ccaagctttg ttggcggcag tgtgcccgcg gcgaggactt
     1081 tttccatgag ccaggcggtg ggaacgattt gtcccggttt tgacagcggg aaacagacgc
     1141 attccggcgc tcccacgctg ccggtcaatg ggccgtggtt gaacgatcaa cacaaccaca
     1201 tcagggacac gccttggatc acttatggca agatgtccac gattcgcgcg cccgggccga
     1261 gcaaactttg ggtgctggtg gatgaagatg tccaagggct taatgacgcg gcattcgcct
     1321 ttgggatgga aaaataccaa tggcgcgacg cgccgggaac gtatcacaac ggcggttgcg
     1381 gtttcgcgtt tgctgacgga cattccgaaa cgcacaaatg gttcagcagc gcggaaaaag
     1441 atgccggctc ggcgatcact gaggaatccg accagcagga ttggctctgg gtgcgcgagc
     1501 ggacttcggc caacatcaac ggcgcaatgc cggccccggt tccttgatgc catattttca
     1561 attgcgcggg gaattttgat ttccggcaag ggtttacttt ccggc
//
LOCUS       DASZMM010000166        10666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1169186, whole genome shotgun sequence.
ACCESSION   DASZMM010000166 DASZMM010000000
VERSION     DASZMM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169186"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VG754_04040"
     CDS             <1..727
                     /locus_tag="VG754_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWF18408.1"
                     /translation="AGIIVEPVPGNAGLYLPRPGYLEFLREITAANGSLLIFDEVMTG
                     FRLARGGAQERFGITPDLSCFGKIIGGGLPVGAFGGRAEIMDYLAPLGPVYQAGTLSG
                     NPVAMAAGMATLNELSSKFKVQSSKSAATDAVDVYDHLEKIGAQLEAGMMDAAKSAKV
                     PVQFRRIGSMFCGYFTDKPVHNLADAMHSDRTRFAKYFHGMLNEGIYLAPSQFEAGFI
                     SAAHTTEDIDKTVKAAAKAMKTL"
     gene            727..1140
                     /gene="ruvX"
                     /locus_tag="VG754_04045"
     CDS             727..1140
                     /gene="ruvX"
                     /locus_tag="VG754_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWF18409.1"
                     /translation="MRTLAIDHGTVRIGLALSDELGIIAQPLEFVLAEPLDKFLARLK
                     EIIQQKEVGLLLVGMPRNMDGSYGPAAAKVKEFVEMLKAAVAVPVKMWDERLTSAQAN
                     RLLIEADVRRDQRKQKVDKMAAAILLQSYLDSQSA"
     gene            1264..2076
                     /gene="truA"
                     /locus_tag="VG754_04050"
     CDS             1264..2076
                     /gene="truA"
                     /locus_tag="VG754_04050"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWF18410.1"
                     /translation="MTPLKFKLTIAYDGTNYAGWQVQKIGVGVQQRVEEALAKLFPGV
                     GRIHSSSRTDTGVHALGMVAHVEIPKKEFRMPAAKLALAINAHLPEDIRVLSARRCAA
                     DFHARFDAAGKQYRYFIWNHNAMNPLLRHQAWQVPRKLDLPAMRSAARLFLGKHDFKS
                     FAASRNYEMESTVRTLNRCEIRRSGPLLTFIIEGDGFLYKMCRGIVGTLAQVGQGKIA
                     ADEIKGILAGKDRRLGGMTAPACGLVLWKVFYKRKHRMLNAEVRTSKTGGAD"
     gene            2073..2552
                     /gene="trmL"
                     /locus_tag="VG754_04055"
     CDS             2073..2552
                     /gene="trmL"
                     /locus_tag="VG754_04055"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HWF18411.1"
                     /translation="MNIVLVEPEIPPNTGNVARLCAATKTRLHLIEPLGFKLDDRQLK
                     RAGMDYWEQVEWHKWPNWAAFVEQLPATARIWFVESNGPLLYSEAKFAPDDYIVFGRE
                     TAGLPKQLLEQNRERWLRIPMFNADSRSLNLSNCAAIVLFEALRQQGFSGEMLDAGC"
     gene            complement(2657..3148)
                     /locus_tag="VG754_04060"
     CDS             complement(2657..3148)
                     /locus_tag="VG754_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWF18412.1"
                     /translation="MQNVVPRRPALVELLSPATIKLPLAGESRDAVLTELVNQIPEIA
                     DRPEDRQTLLRALQEREQLHSTGIGDGVALPHARNALVGLVDRPIIVFGRHDTGIAYS
                     SIDGAPARLFFLLIAPTVTQHLAILARLGRLLRDTKLRKALLVAENSAKVIALIREAE
                     AKM"
     gene            complement(3213..4199)
                     /locus_tag="VG754_04065"
     CDS             complement(3213..4199)
                     /locus_tag="VG754_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWF18413.1"
                     /translation="MSRTSTELHEAAVKRNVDGVRHLLQTRPELVHVRDEKQRTSLQL
                     AARGGCMEVVEMLLASGADVNTTDEKFQTPLQMAAYYGFKDVVSLLLDKGALTNAANR
                     WQITPLHLASNFGHKDVVELLLERGADVNAQESKGQTPIYQAANYGHSRAVKVLLSKG
                     ADVNARDKIGATPLFAAAAYGQRAVAEILITSQADVNAADKGGWTALHHAAISGHQPI
                     AELLISHRGRVDAFDKHGVTPLHIASRVGHVHIVKLLLDNRADINRRNYPGRSALHMA
                     ATCGQTQVVDLLISRRAEVNAPDYSNKTSLYYASNNEHFEIVELLKKSGAKD"
     gene            complement(4362..6272)
                     /locus_tag="VG754_04070"
     CDS             complement(4362..6272)
                     /locus_tag="VG754_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWF18414.1"
                     /translation="MSKIVGIDLGTTNSLVAMVDSGIPVVLADAEGRRLTPSVVHFPS
                     ASAEPVVGHPANRVRVVKPAETVYSIKRFMGRRGNEIAREEMLVTYPVRGEGAGPVTV
                     NIHGQSRTPEEISAEILKKLRRDAEAQLNEPVTRAVITVPAYFNDAQRNATKRAGEIA
                     GFTVERILNEPTAAALAYGLDRLKDKAKIAVFDLGGGTFDLSILELNEGVFQVLSTNG
                     NTRLGGDDLDKRLVDFLVEKIMAAGGPDATKDLPMLSRIRETAEQTKIKLSTETETEI
                     ALPFLTPDFSFNLRFSRAELENLTRDIIAKTRPHCLRALADAKLEAKDLEQIILVGGQ
                     TRMPLVRQMVTELFGCAEFEESRGGIRLGTDYHRAKGPLLNTSQNPDEAVALGAAIQA
                     EILSGGFKNVLLLDVTPLSLGIETFGGLMNVIIHRNSTIPVKAGEMFTTAVDNQKNML
                     IHVLQGERERAKDNWSLGRFTIDFESAPKGVPRIGVQFEIDANGILHVLARDTKTGKQ
                     TVVEMKSAVDVDDADVQRMVEESVEHAFEDMAARRWIEATLRARETLAATRKGLVECA
                     DELDANNRSKIEAAADAVETALATESPQTKTGDTQKIQAAIASLDEATKPLAELLMDR
                     AMEALLRKRGVI"
     gene            complement(6368..8872)
                     /locus_tag="VG754_04075"
     CDS             complement(6368..8872)
                     /locus_tag="VG754_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWF18415.1"
                     /translation="MSDSITSSPMPEQFRAALGLEEGVTLSDGLAMAEERLRDIISKQ
                     QKLPERHPSHPKLKKDRERIEPVIADLHEMVFGLKVDELCDAAEAVLSEKPPRRAVAR
                     DLLKRARLDGVTLGADSVYIRRIQKLEIKLEAEPAPSAFSSKSNVAPVSVAPAPIIKT
                     LPIENPFLQLESLIDEGTTCLLQAPPDLLRAETCYAAAEKDASGRIVPDKLKSRLLVF
                     RSALDRARKEDLRAQVDRILKEVNAMLAGKSPELPQAKQKLIGASTLLERAPDPRLQH
                     ELESLMREVQQFAQHTERRHAESPRMKNVRVQLGTRVRFRCRHRDQESYREVRATEFL
                     IGRGSQGGTACLELSDDLRVSRRHARVWTEGGFFWIEDLGSKFGTKLDGIEIKGAGRR
                     PLNPLSKILLGDTMLVLMEDDTARTPFQPLVAQDASRTPPVHIDSSVDARGVKGEAAE
                     RLLQRQPWLLELHLEMAAQRNLSDLLSLILHRAIEAIATAERGAVLLRDRDKETLLLA
                     AYVSDGEPPVSDSLARKAMKDAMAFMWRRGFDESAESVRRLHIESGIYAPMAWEGRML
                     GVICVDNPRSESAFNADDLRLMQVLAHYAAMAVANQLMRDELRQNAVMLERLLTTFSP
                     ILRNRIVNQAREGRLRPGGLRSEVTILFADLRGFTAATARRDADEVMELLNDYLPAFA
                     QVVFKHDGIIDKFTGDGLLAVFGSPEPDPEQHRHALLAACEMQSVATRITERRTKAGA
                     VACSLGIGLHSGDVLHGFLGGAEMLEFTVIGEAVNLANRFCNTAPPGAVLLSPQVFQH
                     VFQHVRAEPVVVETKHEGKLNGYRVIELKHAPQAAT"
     gene            9231..9881
                     /locus_tag="VG754_04080"
     CDS             9231..9881
                     /locus_tag="VG754_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HWF18416.1"
                     /translation="MKCIVTAGPTYESLDDVRRLTNFSTGRLGSELAAFLIERGHEVT
                     LLLGEQATYRNEHQPGEIQTFTSTENLREKLQALAVGDAQAVFHAAAVSDYKFGRVWV
                     RSPGGEMVQIKSGKISTREGALLAELVPTPKIIAELREWFPNARLVGWKFEVEGNRAE
                     VIRLATNQIMECKTDRAVANGRAYGDGFGLVAADDSHVHLPDKPALFSALEKFIRC"
     gene            complement(9883..>10666)
                     /locus_tag="VG754_04085"
     CDS             complement(9883..>10666)
                     /locus_tag="VG754_04085"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18417.1"
                     /translation="AFYLSDWPGGKIDQSVAVDRSGHAGVARQRCADVLARHDAMGQT
                     VLLFGLLGVYVVNVRHFTSQLVNPFWIHLVSYLNLGACSLNLATLTTRFVYPQFSLEG
                     RRLWIVGMAPMGLPKVVRAKYWLASFSSLLITLSLVVLSSCLLRMPWDRVAFFGAVIT
                     VMTFTLNGLAAGLGVLYPNFKEGNPSKIVSGFGGTFCLVLSFLYILGSVLILAFGAAE
                     MRGKAPSVFWAGVSVCGFFVLSFLIGWLPFRLGLRQLKQFEF"
BASE COUNT         2313 a         3147 c         2991 g         2215 t
ORIGIN      
        1 cgccggcatc atcgttgaac cagttccggg caacgcggga ctttatctgc cgaggccggg
       61 ttatctggaa ttcttgcgcg aaatcacggc ggcaaacggc tcgctgctga ttttcgacga
      121 ggtgatgacc ggattcaggc tcgctcgcgg cggcgcgcag gaacgtttcg gcatcacgcc
      181 ggacttgagt tgcttcggca aaatcatcgg cggcggtttg cctgtcggcg cgttcggcgg
      241 acgcgcggaa atcatggatt atcttgcgcc gctgggacct gtttatcaag cgggcacatt
      301 aagcggcaac ccggtcgcga tggcggcggg catggccact ctgaatgaat tgagttcaaa
      361 gttcaaagtt caaagttcaa agtcggcggc aacagatgct gtcgatgttt atgaccattt
      421 ggaaaaaatc ggcgcgcaac tggaagcggg catgatggac gcggcgaaat cggcaaaagt
      481 gccggtgcaa ttcaggcgca tcggttcgat gttctgcggt tattttaccg acaagccggt
      541 gcataatctc gccgacgcca tgcacagcga tcgcacgcgg ttcgcaaaat attttcacgg
      601 catgttgaac gagggcatct accttgcccc atcgcaattc gaagccgggt tcatttccgc
      661 cgcgcatacg actgaagata ttgataaaac cgtgaaagcg gcggcgaaag caatgaagac
      721 gttataatgc gcacacttgc catagaccat gggaccgtcc gaatcggact cgcgctcagc
      781 gatgaattgg gcatcattgc gcagcctctc gaatttgtgc tggcggagcc tttggataaa
      841 tttctcgcgc gattaaagga aatcatccaa cagaaggagg tcggattgct gctggtcggg
      901 atgccgcgca acatggatgg cagttacggc ccggcggcgg cgaaggtgaa ggaatttgtg
      961 gaaatgttga aggccgccgt tgccgttccg gtcaagatgt gggacgagcg gctgacttcg
     1021 gcgcaggcga accggctttt gatcgaggcc gatgtgcgcc gcgatcagcg caaacaaaaa
     1081 gtggacaaga tggccgcggc gattttgttg cagagctatc tcgacagcca gagtgcttga
     1141 tttttagcca cggatgaaca cggaataaac acggatggaa gacacgaatt gcacgaatca
     1201 tcacgaatca aggaaacgca aaaattcgtg tcaattcgtg aaattcgtgt ctgtgttcac
     1261 ctcatgaccc cgcttaaatt caagctcacg atcgcttacg acgggacgaa ctatgccggc
     1321 tggcaggtgc agaagatcgg cgtgggcgtg caacagcgcg tcgaggaagc gctggcaaaa
     1381 ttgtttcccg gagtcgggcg catccacagt tccagccgga cggacaccgg cgtccatgcg
     1441 ctcggaatgg tcgcgcatgt ggaaattcca aaaaaagaat tccgcatgcc cgcagccaag
     1501 ctcgcgctcg ccatcaacgc gcatttgccg gaggacatcc gcgtcctttc agcgcggcga
     1561 tgcgcggcgg attttcacgc gcggttcgac gccgcaggca agcagtatcg ctatttcatc
     1621 tggaaccaca acgcgatgaa tccgcttttg cgccatcagg cgtggcaggt cccgcgcaaa
     1681 ctggatttgc cggcgatgcg ctcggcggcg cggctttttt tgggcaagca cgatttcaaa
     1741 tccttcgcgg caagtcgaaa ttacgaaatg gaatccaccg tgcgcacgtt gaaccgatgc
     1801 gaaatccggc gcagcggtcc gttgctgacg ttcatcatcg aaggggatgg ttttctttac
     1861 aaaatgtgcc gtggcatcgt cggcacgctg gcgcaagtgg gccagggaaa aattgcggca
     1921 gacgaaatca agggcatcct tgccggcaaa gaccgccggc tcggcgggat gacagcgccc
     1981 gcgtgcggat tggttttgtg gaaggtgttt tacaaacgaa aacatcgaat gctgaacgcc
     2041 gaagtccgaa catcgaaaac cggaggtgcg gattgaacat tgttctcgtg gaaccggaaa
     2101 tcccgccgaa cacgggcaac gtggcgcgct tgtgcgcggc gacgaaaacg cgtctgcatt
     2161 tgatcgagcc gcttggattc aaactggacg accggcaact caaacgcgcg gggatggatt
     2221 actgggaaca ggttgaatgg cacaaatggc cgaactgggc ggcgttcgtg gaacaacttc
     2281 cggcgactgc gcgaatctgg ttcgtggagt cgaacggtcc cctgctctat tcagaggcga
     2341 agtttgcgcc ggatgattac attgtgtttg gccgcgaaac ggcgggattg ccgaaacaat
     2401 tgctcgaaca aaaccgcgaa cgttggctgc gcatcccgat gttcaacgcc gattcgcgct
     2461 cgctgaatct ttcaaactgc gctgcgattg tgctctttga ggcgttgcgt cagcaggggt
     2521 tttctggtga gatgcttgat gctggatgtt gagctctacg agcgaatcat atcagacggc
     2581 tcgcaaggtc tttccagacg gcctgcgctc gccccacgtg atccagcatc gagcatccag
     2641 aaaccagcat ccagccttac atcttcgctt ccgcctcgcg gatcagcgcg atgaccttgg
     2701 cggagttttc agcgacaagg agcgccttgc gcaattttgt gtcgcgcaat aaccggccta
     2761 gccgcgcgag gatggcaaga tgttgcgtaa cggtcggcgc gatgagcaga aagaacaaac
     2821 gcgccggcgc gccatcaatc gacgaatatg caatgccggt gtcatgcctg ccgaacacga
     2881 tgatgggacg gtccacaagg ccgaccaacg cgttgcgcgc gtgaggcagc gccacgccgt
     2941 cgccgatgcc ggtgctgtgc agttgttcgc gttcctgtaa tgcgcgcaac aacgtctgcc
     3001 ggtcttcggg gcggtcggcg atttcgggga tttgattgac gagttcggtc agcaccgcgt
     3061 cgcggctttc gccggcaagc gggagcttta tcgtggcggg cgacaacaat tccaccagcg
     3121 ccggacgtct tggaacaaca ttttgcatac gaggaaatct tacgaacgac ttgggcggaa
     3181 ggagaaggaa aatgttcggc caacaaaaaa aattaatcct tcgcgcccga tttcttgagc
     3241 agttccacga tctcgaagtg ctcgttgttg ctcgcataat acagcgaggt cttgttgctg
     3301 taatccggcg cgttgacttc ggcacggcgg cttatgagca ggtccaccac ttgggtctgg
     3361 ccgcaggtcg ccgccatgtg aagcgcggaa cgccccggat aattccggcg attgatgtcc
     3421 gcgcggttgt cgaggagcag cttgacgatg tggacatggc cgacgcgcga ggcgatgtgc
     3481 agcggggtga cgccgtgctt gtcaaacgcg tccacgcgcc cgcgatgtga aatcagcaat
     3541 tccgctatcg gctgatggcc ggaaatcgcg gcatgatgca acgcagtcca accgcccttg
     3601 tccgcggcat tcacgtccgc ctggctggtg atgagaattt ccgcgacagc cctttgccca
     3661 taagccgccg cggcgaacaa cggcgtcgcg ccgattttgt cccgcgcgtt gacgtccgct
     3721 cccttggaaa gcaacacctt cacagcacgg ctgtgcccgt aattggcagc ctgataaatc
     3781 ggggtttggc ccttgctttc ctgggcgttc acgtccgcgc cgcgttcgag cagcaattca
     3841 acgacatctt tgtggccgaa attggaagcg agatgaagcg gggtgatttg ccagcggttg
     3901 gccgcgttgg tcagcgcgcc cttgtcgagc agcaacgaca ccacgtcttt gaaaccgtaa
     3961 taagcggcca tctgcaaggg cgtttggaac ttctcgtcag tggtgttgac gtccgcgccg
     4021 gacgcgagaa gcatttcaac gacctccatg caaccgccgc gagcggccag ttgcaaagaa
     4081 gtccgctgct tttcgtcccg aacgtgaacc agctcgggcc gggtttgaag cagatgccgg
     4141 acgccgtcca cgttgcgctt tacggctgct tcatgaagtt ctgttgatgt cctgctcaaa
     4201 gtgctttttt ctagtcacca ttaatattga agattacaag gctctgcaac agcattataa
     4261 tccgaaagcc agtaagaacg ggtttatgat taaaaattaa tctgattatg tcttgtaggg
     4321 caatacccta catcaacgga ttggtattta aaatttcttt cctatatcac tccgcgtttc
     4381 cgcaataacg cttccatagc cctgtccatc aacaactcag ccagaggttt tgtagcttca
     4441 tccaggctgg caatggcggc ttgaattttt tgcgtgtcgc cggttttggt ctggggcgat
     4501 tccgttgcca acgcagtctc gactgcgtca gcagcggctt caatcttcga ccgattattc
     4561 gcatctaatt catccgcgca ttcgacaagg cctttgcgcg tggccgcaag agtttcacgg
     4621 gcgcgaaggg tcgcctcgat ccagcgccgc gccgccatgt cctcaaaggc gtgttcgacc
     4681 gattcctcga ccatgcgctg cacgtcggcg tcatccacgt ccacggcgga tttcatttca
     4741 acgacggttt gttttcccgt cttggtgtcg cgcgcgagca cgtgcaggat gccgttggcg
     4801 tcaatctcga attgcacgcc gatgcgcgga acgcctttgg gcgccgattc aaaatcaatg
     4861 gtgaaacggc cgagactcca attgtccttc gcgcgttcgc gttcgccctg gagcacatgg
     4921 atgagcatgt tcttttggtt gtccaccgcg gtggtgaaca tctcgccggc tttcaccgga
     4981 atggtggaat tgcgatgaat gatcacgttc atcaacccgc cgaatgtctc gatgcccagc
     5041 gacaagggcg tcacatccag cagcagcaca ttcttgaaac cgcccgaaag aatttccgcc
     5101 tggatggccg cgcccaacgc gaccgcttcg tccggatttt gcgaggtgtt gagcaacggt
     5161 cctttggcgc ggtgataatc cgttcccaaa cggatgccgc cgcgcgattc ctcgaattcc
     5221 gcgcacccaa aaagttccgt gaccatctga cgcaccaatg gcatgcgcgt ttgacccccg
     5281 acaaggatga tttgttccaa atctttcgcc tccagcttcg catccgccag cgcgcgcagg
     5341 caatgcggac gcgttttggc gatgatgtcg cgcgtgagat tttcgagttc ggcccggctg
     5401 aagcgcaaat tgaaactgaa atcgggcgtg agaaatggca gcgcgatttc agtttcggtt
     5461 tcggttgaaa gcttgatctt ggtctgctcg gcggtttcgc ggatgcgcga aagcatcggc
     5521 aaatctttcg tcgcatcggg ccctcccgcg gccattattt tttcgaccag aaaatcaacc
     5581 aatcgcttgt cgagatcatc gccgcccagt cgcgtgttgc cattggtgga caacacctgg
     5641 aagacgcctt cgttcaattc aaggatcgaa aggtcaaatg tgccgccgcc caaatcgaag
     5701 acggcgatct tcgccttgtc ctttagccgg tcaagaccgt aggcaagcgc ggcggcagtg
     5761 ggttcgttca ggatgcgttc gacagtgaag ccggcgattt cgcccgcgcg tttggtggcg
     5821 ttgcgctgcg cgtcattgaa ataagcaggc acggtgatca ccgcgcgggt aaccggctca
     5881 ttcaattgcg cctcggcatc acggcgcaac ttcttcaaga tttctgcgga aatctcctcg
     5941 ggcgttcgcg attgcccgtg gatgttgacc gtaaccggcc ccgcgccttc gccgcgcacg
     6001 ggatacgtta ccaacatttc ttcgcgggcg atttcattgc cgcgccggcc catgaaacgc
     6061 ttgatggaat aaaccgtctc ggcgggcttc accacgcgga cgcgattggc cgggtgtccc
     6121 acgaccggtt cggcgctcgc gctgggaaaa tgtaccaccg acggcgtcaa tcgccttcct
     6181 tcggcatcgg caaggaccac aggaatcccg gaatccacca tcgccacgag tgaattcgtg
     6241 gtgccgagat caatgccgac aatcttgctc attccgataa tgatgccacg gtcgggcatg
     6301 ggatgaaagc cgattgaggg gataacaaaa aattcttcct caaaaaatga accggccaac
     6361 gaatgactta agtcgccgct tgcggcgcgt gcttcaattc gatcacccga tagccgttca
     6421 attttccttc gtgcttggtt tccaccacca ccggctcggc gcggacgtgt tggaaaacgt
     6481 gttggaaaac ttgcgggctc aggagcaccg cgccgggcgg cgcagtgttg caaaaacgat
     6541 tggcgagatt gactgcttcc ccgatgaccg tgaactcaag catctccgcg ccgccaagaa
     6601 aaccatggag cacgtccccg ctgtgcaggc cgatgcccaa actgcacgcc accgcgccgg
     6661 cttttgttcg ccgctcggtg atgcgcgtgg cgaccgactg catctcgcag gcggccagca
     6721 acgcgtgccg gtgctgctcg gggtccggtt ccgggctgcc gaacaccgcg agcaatccgt
     6781 ccccggtgaa cttgtcaatg atgccatcat gcttgaagac cacctgggca aacgccggca
     6841 aataatcatt caacaattcc atcacttcat ccgcatcgcg acgcgccgtc gccgcagtga
     6901 agccgcgcaa atcggcaaaa agaatcgtca cttccgaacg caatccgccc ggacgcaacc
     6961 ggccttcacg cgcctggttg acaatccggt tgcggagaat cggtgaaaag gtggtgagaa
     7021 gccgttccag catcacggcg ttctggcgca gttcatcccg catcaactgg ttcgccaccg
     7081 ccatcgccgc gtaatgcgcc agcacttgca tgagccgcag atcgtccgcg ttgaaagcgg
     7141 attcgctcct cggattgtcc acgcagatga cgccgagcat ccgcccttcc cacgccatcg
     7201 gcgcgtaaat gcccgattcg atgtgcaacc gccgcacgct ttccgccgac tcatcgaatc
     7261 cgcgccgcca catgaaggcc atcgcatcct tcatggcttt tctcgcaagg ctgtcgctca
     7321 ccggcggctc gccatccgac acataagccg ccagcaggag cgtttccttg tcgcgatcgc
     7381 gcaacaacac cgcgccgcgt tccgctgtgg caatggcctc gatcgcgcgg tgcaaaatca
     7441 gggacaacaa atcggaaaga ttgcgttgcg ccgccatttc caagtgcaac tcaagcaacc
     7501 acggctgccg ttgcagcaat cgctcggccg cttcgccttt caccccgcgc gcatcgacgc
     7561 tcgaatcaat gtgcaccggg ggcgttcgcg aagcgtcctg cgcaaccagc ggttggaaag
     7621 gcgttcgcgc ggtgtcgtcc tccatcagca ccagcatggt gtcgccgagc aaaatttttg
     7681 agagcggatt gagcggacgc cgccctgccc ctttgatttc gatgccgtcg agttttgttc
     7741 caaatttgct gccgaggtct tcgatccaaa aaaatccgcc ctcggtccag acgcgcgcgt
     7801 ggcggcggga aacgcgcaaa tcatccgaaa gctccaggca ggctgttccg ccttggctgc
     7861 cgcgcccgat gaggaattcc gtggccctga cttcgcgata actttcctgg tcgcgatgac
     7921 ggcaacggaa gcgcacgcgc gtgccgagtt gcacgcggac atttttcatg cgcgggcttt
     7981 ccgcatggcg gcgctcggtg tgctgcgcga actgctggac ttcccgcatc aagctttcga
     8041 gttcatgctg caaacgcggg tcgggcgctc tttcgagcag cgtggaagcc ccgatcaatt
     8101 tctgtttcgc ctgcggaagt tccggcgatt tccccgcgag catggcattc acctccttca
     8161 agatacggtc cacttgcgcg cgcaaatcct ccttcctcgc gcggtccagc gcgctcctga
     8221 aaacgagcaa cctgcttttg agcttgtcgg gaacgattct cccggaagcg tccttctccg
     8281 ctgccgcata acacgtttct gctcgcaaca aatccggcgg cgcttgcaac aaacaggtgg
     8341 tgccttcgtc aatcaacgat tccaactgca agaatggatt ttcaatgggc aatgttttga
     8401 tgatgggcgc aggcgcaacg ctcaccggcg caacattcga cttcgaagaa aatgcggatg
     8461 gcgccggctc ggcttccaac tttatctcca atttttggat gcgccttata taaacactgt
     8521 cagcccccag cgttactcca tcgagacggg cgcgtttgag caagtcgcgc gcgacggccc
     8581 ttcgcggcgg cttttcgctc agcacggctt cggcggcgtc gcaaagctca tcgaccttca
     8641 ggccgaaaac catctcgtgc aaatcagcga tcaccggctc gatgcgctcg cggtctttct
     8701 tcagcttggg atgcgacgga tgtctctcgg gcaatttctg ttgcttcgat ataatgtccc
     8761 gcaaccgctc ctccgccatc gcaagcccat ccgagagagt cacgccttct tcaagcccca
     8821 acgcggcgcg aaactgttcc ggcatcggcg acgatgtgat gctgtcactc atcgcaatcc
     8881 tccgaaaaat taaatcgcgc cttgctgggc cgcatctcaa tttcttgcac gatgaagtga
     8941 tcggacgttt ccttcccctc accccaaccc tctccctcag ggagagggag caacgcattc
     9001 cgtcgtggtt gcaatccgac ggcgttcaac ttttcgggca gcggaaaaat ggttccccct
     9061 ctcctggggg agagggacgg ggtgaggggg acagcgatgt gcgccagccc atcaacacaa
     9121 tccgcaaggg cctggaaaag cgcaaaatag tgagatgcac ccgccctgtc acgaccatgc
     9181 cgtaattttg attttccaat gcgccaaaaa cccgctacgc tccgcctgcc atgaagtgca
     9241 tcgtcacagc cggccccact tacgaatcgc tcgatgacgt ccgccggttg accaattttt
     9301 ccaccggcag gctcggctcg gaactcgccg catttctcat cgaacgcggt catgaagtga
     9361 ctttgctgct cggcgaacag gccacttatc gcaacgaaca tcaacccggc gaaatccaga
     9421 ccttcaccag cacggaaaat ttgcgcgaaa aattgcaggc gctcgcggtg ggtgacgcgc
     9481 aagccgtttt ccacgccgcc gcggtgagcg attacaagtt tggcagggtt tgggtccgtt
     9541 cgcccggcgg tgaaatggtg caaatcaagt cgggcaaaat ttcgacacgc gagggcgcgt
     9601 tgctggccga gttggtgccg acgccgaaaa tcatcgctga attgcgcgaa tggtttccca
     9661 acgcccgcct cgtcggctgg aaattcgagg tcgaaggcaa ccgcgccgaa gtcatccgac
     9721 tcgccacgaa ccaaatcatg gaatgcaaga ccgatcgcgc cgtcgccaac ggccgcgctt
     9781 atggcgacgg tttcggattg gttgcagccg atgacagtca tgtgcatctg ccggacaagc
     9841 cggccttgtt cagcgctctt gaaaagttca tccgttgttg aatcaaaatt caaactgctt
     9901 caactgccgc agcccaagcc tgaacggcag ccagccgatc aggaacgaaa gaacgaaaaa
     9961 tccgcaaaca ctcacccccg cccaaaaaac cgacggcgct ttcccgcgca tctccgccgc
    10021 gccgaatgcc aaaatcaaaa ccgagccaag gatgtagaga aaactcaaca ccaggcagaa
    10081 cgtcccgcca aagccgctga cgatcttgct cggatttcct tccttgaaat tcggatacag
    10141 cacgcccaac cccgccgcaa ggccgttcaa cgtgaacgtc atcaccgtta tcaccgcgcc
    10201 aaaaaaagcc acgcggtccc acggcatccg caacaggcaa ctcgacaaca ccaccagcga
    10261 aagcgttata agcaacgacg aaaaacttgc cagccaatac ttggctctca cgaccttcgg
    10321 caaacccatc ggcgccatgc cgacgatcca caatcgccgt ccttccagcg aaaattgcgg
    10381 ataaacaaac cgggtcgtga gcgtcgcaag gttcagcgag cacgcgccca aattgaggta
    10441 cgaaaccagg tgtatccaga acggattcac aagctggctg gtgaaatgcc gcacattgac
    10501 cacatacacg cccagcaagc cgaacaacaa aaccgtctgc cccattgcgt cgtgtcgcgc
    10561 caaaacatcc gcacatcttt gacgagcaac gcccgcgtgt ccggagcgat ccaccgcaac
    10621 agattgatca atttttccgc cgggccaatc ggataggtaa aaggca
//
LOCUS       DASZMM010000167         3251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1718070, whole genome shotgun sequence.
ACCESSION   DASZMM010000167 DASZMM010000000
VERSION     DASZMM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718070"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VG754_04090"
     CDS             <1..505
                     /locus_tag="VG754_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--alanine ligase"
                     /protein_id="HWF18418.1"
                     /translation="LRMNAGAMGGWMFDVVESIRYMDYTGQAHEEMASKVPVEYRSCP
                     LLKDHIALGAVLKGHPASKDVVEKRMKTFSQKRWESQPAAPSAGCIFKNPGTIPAGKL
                     IDELGLKGMRVGDAMVSQEHGNFIVNQGHANAKDVLELIEVIRQKAREKRGIELQTEV
                     EIIGEAS"
     gene            738..1658
                     /locus_tag="VG754_04095"
     CDS             738..1658
                     /locus_tag="VG754_04095"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWF18419.1"
                     /translation="MSKSLNITVMLGGPSAEREVSLRTGKAVAKALRSLGHEVEELDP
                     KEPAWTLSPKTQVVFLALHGTYGEDGTVQRLLDELDIPYTGCDAEASRIGFDKFLTKQ
                     KCVEAGVPTARFLVLESTNEGWPTGWEPPIVLKPVRQGSSVGLQFVESVENWDAALAE
                     ALRHDSQVLMEEKIVGRETTVAILADKALPVIEVRPKSGNFDYKNKYTPGATEVFCPA
                     QLDEATVKRVQAAAMGAFKAIGGRDYARVDVMLRPNGEPVVLEVNTLPGMTELSLFPK
                     AAAAAGIDYPELCQRMVELALHHNSGAAKK"
     gene            1655..2617
                     /locus_tag="VG754_04100"
     CDS             1655..2617
                     /locus_tag="VG754_04100"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWF18420.1"
                     /translation="MSWFRRKPKNRRLGHAQVLDVKLRSDQVRANRMRVTAVALGLVF
                     ATIVGLYVIWRAGEFTLNRLVYENKSFAVQEIDVRTDGVIAQDQLRRWAGVRIGENLL
                     ALDLARVKRDLEMVSMIRSVAVERVLPHTLRLRVTERDPLAEVRVLQPRAGGGVQMAV
                     LQVDADGFVMTQVDPRQRATPATQTNDVLPLITGVNPNELLPGRRLESQQARSALQLL
                     LAFDRSPMSGLVDLQTIDVSAPQVLQVTTSERSQITFSVQDLDQQLRRWREVHDQAAR
                     LGKVIVTFDLSVHDSVPLTLIEAGAAPPATPKMKNPQRKGRRNV"
     gene            2610..>3251
                     /gene="ftsA"
                     /locus_tag="VG754_04105"
     CDS             2610..>3251
                     /gene="ftsA"
                     /locus_tag="VG754_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWF18421.1"
                     /translation="MFDPASLIVGLEIGTSKVVAVVGEVNAGGSLNLIGLGQARSRGV
                     RKGEITDVSAVEEDVRNAIVEAEQMADVEIRSVYLGVTGAHIRGFNNRGVHPVVSADR
                     EITAEDVQDVIKNAKAINLPTENHVLHAVRQHFLVDGQDGIADPVGMLGARVEVDVHV
                     VHGNFNRLQNPIRVVKGLQLEVEAIVFNGLASSLALLTNEQKELGALVIDIGGG"
BASE COUNT          709 a          815 c         1055 g          672 t
ORIGIN      
        1 attgcgcatg aacgccggcg cgatgggcgg ctggatgttc gacgtggtgg aatccatccg
       61 ttacatggat tacaccggcc aggcgcacga ggaaatggcc tcgaaggtgc cggtggaata
      121 ccggagttgc ccgctgttga aagaccacat cgcgctgggc gcagtgctca aggggcatcc
      181 ggcatcgaaa gatgtcgtcg agaaacggat gaaaaccttc agccaaaaaa gatgggaatc
      241 ccagccggct gcgccgagtg cgggttgcat tttcaagaac cccgggacca taccggcggg
      301 caagctgatt gatgaactcg gtttgaaagg catgcgggtg ggagacgcca tggtatcgca
      361 ggagcacggc aacttcattg tcaaccaggg tcacgcgaat gcgaaggatg ttttggaact
      421 catcgaagtc atccgccaaa aggcgcgcga gaaacgagga attgagttgc agacggaagt
      481 cgagatcatc ggagaagcgt cctaattgcg gattgctgat ttcggattgc ggattttttt
      541 ggatggtcaa gctgtgtggt tttcgttcgg cggttataca actgtttcat gattgcaaaa
      601 gtgtttagta cgaatgcgaa agcatttgca ttgcgagcaa ttcatttggt tgaatcactc
      661 gcgaacacac ggacttcgga tgccttcaac tcggaaaatt atgcgcattc cgcaatccgg
      721 aatcagatat ggcaacaatg tcaaaaagtt tgaacataac ggtgatgttg ggcgggcctt
      781 cagcggagcg cgaagtttcg ttgcgaacgg gcaaggctgt ggcgaaggca ttgcgttcgc
      841 ttgggcatga agtcgaggaa cttgatccca aggagccggc atggacgttg tcgccgaaga
      901 cacaggtggt ttttctggcg ttacacggga cttacggcga agacggcacc gtgcaacgcc
      961 tattggatga actcgatatt ccgtacaccg gctgcgatgc cgaagcgagc cggattgggt
     1021 tcgacaaatt tttgacgaag cagaaatgcg tcgaagccgg cgtgccgaca gcgcgatttc
     1081 ttgtgctgga atccaccaac gaaggctggc cgacaggttg ggaaccgccg atcgttttga
     1141 aaccggtgcg gcagggttcg agcgtgggat tgcaattcgt cgaaagcgtg gagaattggg
     1201 acgccgcgct ggccgaggcg ttgcgccatg attcgcaggt attgatggag gaaaaaattg
     1261 ttgggcggga aacgacggtt gcgattttgg ccgacaaggc tttgccggtc atcgaagtgc
     1321 gtcccaagag cggcaatttt gattacaaaa ataaatacac acccggcgcg acggaggttt
     1381 tctgcccggc gcaactggac gaggcgaccg tgaaacgcgt ccaagccgcg gcgatggggg
     1441 cgttcaaggc catcggcgga cgggattacg cgcgagtgga tgtgatgctc cggcccaacg
     1501 gcgaacccgt cgtgctggaa gtgaacacgc tgccgggaat gaccgagttg agcttgttcc
     1561 cgaaagcggc ggcggcggcg ggaattgatt atcccgaatt gtgccagcgg atggttgaac
     1621 tggcgttgca ccacaattcc ggagcggcaa aaaaatgagc tggttccgac gcaaaccaaa
     1681 aaatcggcgg ttggggcacg cgcaggtgct cgatgtgaaa ctgcgctccg atcaggtgcg
     1741 cgccaatcgc atgcgagtca cggcagtcgc gctcggattg gtctttgcga ccatcgtcgg
     1801 actttacgtc atctggcgcg cgggcgaatt tacattgaac cggctggttt atgaaaacaa
     1861 atcgttcgcg gtacaggaaa ttgatgtgcg gacggatgga gtgattgccc aagatcaact
     1921 gcgccgctgg gcgggcgtga gaatcggtga aaatcttctc gcgcttgatt tagcgcgggt
     1981 gaagcgggat ttggaaatgg tttcgatgat ccgttcggtt gccgtcgagc gtgtgctgcc
     2041 gcacacattg cgattgcgcg tcacggaacg cgacccgctg gcggaagtcc gcgttctgca
     2101 accgcgcgcg ggcggcggtg tgcagatggc agtgctgcaa gtggacgccg atggttttgt
     2161 gatgacgcaa gtggacccgc gccagcgcgc aacgcctgcc acgcaaacga acgatgtttt
     2221 gcctttgatc accggcgtca acccgaatga acttctgccg ggcaggcgct tggaatcgca
     2281 acaggcgcgt tcggcgttgc aattgcttct ggcattcgac cgttcgccga tgtcggggct
     2341 tgtggatttg cagacgattg atgtttccgc gccgcaagtt ttgcaggtga cgaccagcga
     2401 acgcagccag atcacttttt ccgttcagga tttggaccag cagttgcgcc gctggcgcga
     2461 ggtgcatgac caggcggcgc gcctcggcaa ggtcatcgtc acgttcgatt tgtccgtgca
     2521 tgacagcgtg ccgctcacct tgatcgaggc gggcgctgcg ccacccgcca cgccaaaaat
     2581 gaaaaaccct caacggaaag gcaggagaaa tgtttgatcc cgcttctttg atcgtcggac
     2641 tggagattgg aacgtccaag gtcgtggccg tcgtcggcga agtgaacgcc ggcggttcgc
     2701 tgaacttgat cggtcttggc caggcgcgct cgcgcggcgt ccgcaagggt gaaatcaccg
     2761 acgtctcggc agtcgaggaa gacgtgcgca acgccatcgt cgaggccgaa caaatggccg
     2821 acgtggaaat ccgcagcgtt tatctcggcg tgactggcgc gcacatccgc ggattcaaca
     2881 atcgcggggt tcatcccgtc gtttccgccg accgcgaaat caccgctgag gacgtgcagg
     2941 atgtcatcaa gaatgccaag gccatcaacc tgccgaccga aaaccatgtt ttgcacgcgg
     3001 tccggcagca ttttctcgtg gacggtcagg atggcatcgc cgacccggtc gggatgcttg
     3061 gcgcgcgcgt ggaagtggac gttcacgtgg tgcatgggaa tttcaaccgg ctgcaaaatc
     3121 cgatccgcgt ggtgaagggt ttgcaattgg aagttgaggc gattgttttc aacggcttgg
     3181 cgtcgtcgct cgcgttgctc acgaacgagc agaaggaatt gggcgcgctg gtgattgaca
     3241 tcggcggtgg g
//
LOCUS       DASZMM010000168         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1718137, whole genome shotgun sequence.
ACCESSION   DASZMM010000168 DASZMM010000000
VERSION     DASZMM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718137"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="VG754_04110"
     CDS             <1..1671
                     /locus_tag="VG754_04110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HWF18422.1"
                     /translation="VPFMALTATATERVRADIVLHLHLREPLCYVASFNRPNLTYRVM
                     AKAGPYEQTLEFIRSRPRESGIVYCHSRKSAEITAERLNADGVSARPYHAGLGTEERS
                     RHQELFLRDEVRVICATIAFGMGINKPNVRFVIHYDLPKNIEGYYQETGRAGRDGLPG
                     ECLLLFSAGDVVKQTQFITEKPDPKEQQIAREQLQGMVHYAECAVCRRRELLRYFGED
                     FTKESCGACDNCLSPRATYDGTLAAQKFLSCVYRIRERSQFGVGLNHVVQVLTGAGTE
                     KIRRWKHDQLSTYGIGKEFSRPEWGAIGRELVRLGYLRQTAEQFSVLELTAEGRAILS
                     KRQTITLTRPVAAPESKAHRAGEINCDEVLFERLHQLRKKLADERDLPGHIIFSDVAL
                     RQMAREYPANEREFARISGVGERKLREFGAVFLREIAAHLEANPRQIFADDSFTGPQP
                     GPKPRLNDTSRATLQSFRAGQSVEQIAQARGFALSTIYGHLSVAVETGEPLDIHQLLT
                     PEQQRQIAAAFAQTGFANLTGAKELLGPNFDYGQLRLYRALKNAGKAS"
     gene            complement(1747..>2265)
                     /locus_tag="VG754_04115"
     CDS             complement(1747..>2265)
                     /locus_tag="VG754_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWF18423.1"
                     /translation="DIRQVSRALGIEEHGREVLRALKNRVVSIIEKTCVITKRPSVAC
                     IEWIEPLMAAGNWVPELVELAGGADVLGEAGKHSAWMDWEALRRADPEIIVAMPCGFD
                     LERTRSEMAVLMRHKDWSKLNAVKKKRVYLTDGNQYFNRPGPRIVESLEILAEIVHPE
                     IFKFEHKGWERC"
BASE COUNT          494 a          713 c          608 g          450 t
ORIGIN      
        1 gtgcctttca tggcattgac cgcaaccgcc acggaacgcg ttcgcgccga catcgtcctt
       61 catctgcatt tgcgcgagcc gctctgctac gtcgccagct tcaaccggcc caatctcacc
      121 taccgcgtca tggccaaggc aggtccctat gaacagacgc tcgaattcat ccgctcgcgt
      181 cctcgcgaaa gcggcattgt ctattgtcac agccgcaagt ccgctgaaat caccgcggaa
      241 cggctgaatg cggacggcgt ttcggccagg ccgtaccacg ccggcctcgg cacggaggaa
      301 cgctcgcggc atcaggaatt atttttgcgc gacgaggtgc gcgtcatctg cgcgaccatc
      361 gctttcggca tgggcatcaa taaaccgaac gtccggttcg tcattcatta tgatttgccc
      421 aagaacattg agggttatta ccaggaaact gggcgcgccg ggcgcgacgg cctgcccggc
      481 gaatgcctcc tgcttttcag cgccggcgat gtcgtcaagc aaacccaatt catcaccgaa
      541 aaacccgacc caaaggaaca gcaaatcgcg cgcgagcaat tgcagggaat ggtgcattac
      601 gccgaatgcg ccgtctgccg gcgccgggag ttgttgcgct atttcggcga ggatttcacg
      661 aaagaatcct gcggcgcatg cgacaactgt ctttcgcccc gcgcaacata cgatggcaca
      721 ctcgccgcgc aaaaattcct ttcgtgcgtt tatcgcatcc gggagcgaag ccagttcggc
      781 gtcggtctca atcatgtggt ccaagtcctg accggcgcgg gcacggaaaa aatacgccgt
      841 tggaagcacg atcaactttc cacgtacggc atcggcaagg aatttagccg tcccgaatgg
      901 ggggcgattg gccgtgaatt ggtccggctc ggctatctgc gccagaccgc ggaacaattc
      961 agcgtcttgg aattgaccgc tgaaggccgg gcaatcctca gcaaacgaca gacaataacg
     1021 cttacccggc ccgttgccgc gcccgaatcg aaggcgcacc gcgcgggcga aatcaattgc
     1081 gacgaggtct tgttcgagcg cttgcaccag ctccgcaaaa aattagcgga tgaacgggac
     1141 ttgcccgggc atattatttt ctcggacgtt gccttgcgcc agatggcgcg cgaatatccg
     1201 gcgaacgaac gcgagttcgc gcgcatcagc ggcgtcggcg aacggaagct gcgcgaattc
     1261 ggcgcggtgt ttctccgcga gattgccgcc caccttgagg cgaatccacg gcagattttt
     1321 gcggacgatt ccttcaccgg gccgcaaccc ggacccaaac cgcgcctcaa cgacacaagc
     1381 cgagcgacac ttcaaagctt tcgcgccggc cagtcggtcg agcaaatcgc ccaggcccgc
     1441 ggcttcgccc tgagcacgat ttacggccat ctcagcgtcg ccgttgaaac gggcgaacct
     1501 ctggacatcc accaacttct cactccggag caacaacgcc agatcgcggc tgcgtttgcc
     1561 caaaccggct tcgccaacct gacgggcgcg aaggaactcc tcggtccaaa ttttgattac
     1621 ggccaactga ggctttatcg cgcgttgaag aatgcaggaa aggcgagtta aaaatatcca
     1681 taccccaaga agcagcaagc cagtcccaat gcgttgcggg aaatttgcgc cggtcggttt
     1741 aggtgtttaa caacgctccc atcccttgtg ctcgaacttg aaaatttccg gatggacgat
     1801 ttcagcaaga atttccagcg attcaacgat gcgcggaccg ggccggttga aatactggtt
     1861 gccatcagtc aaataaacgc gtttcttttt taccgcgttt aattttgacc aatctttgtg
     1921 gcgcatcaaa acagccatct cggaacgcgt ccgttccagg tcaaaaccgc acggcatcgc
     1981 cactatgatt tccgggtcag cgcgccgcaa cgcctcccaa tccatccacg cggagtgttt
     2041 gcctgcctcg ccgagcacgt ccgcgccgcc agcaagttcg accagttccg gcacccagtt
     2101 gcctgcagcc atcaacggtt caatccattc aatgcacgcc acagacgggc gtttcgtgat
     2161 cacgcacgtc ttctcgatga tgctcacgac gcgatttttc aatgcgcgca aaacttcgcg
     2221 accgtgctcc tcgatgccca gcgcccttga gacctgccgg atgtc
//
LOCUS       DASZMM010000169         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3367130, whole genome shotgun sequence.
ACCESSION   DASZMM010000169 DASZMM010000000
VERSION     DASZMM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3367130"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VG754_04120"
     CDS             <1..561
                     /locus_tag="VG754_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18424.1"
                     /translation="VMKILGSILIGVAILIFVTTLVLVFTSPGYFGSRYLRKLMETEF
                     RKRSKPLVSTADADTRFVQVIPRANWAKLKLIDATDVGFLRVDKQRGELLFEGDKECY
                     RIPAAAITSCEIETFIQGQGSHGATTLYRVVLQANHPSGFWEAPFAQCGNSGKYRRKS
                     REKWALALQGEIRELMFTAKNQATPV"
     gene            complement(571..1107)
                     /locus_tag="VG754_04125"
     CDS             complement(571..1107)
                     /locus_tag="VG754_04125"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWF18425.1"
                     /translation="MSIATKTGDKGTTGLMYNRRVSKCHPRVEAYGSVDELNAALGLA
                     RATAEHDFVRDNLLPIQKDLVILMGELATMVEDLPRYAKDGFSLVMPELTAKLDKLVV
                     EIESQKIAFKGWATPGASVHSAALDVARTVCRRAERRVCALHEAGQLQNPEIIIYLNR
                     LADLLWLFARWVETKAQK"
     gene            1196..1438
                     /locus_tag="VG754_04130"
     CDS             1196..1438
                     /locus_tag="VG754_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18426.1"
                     /translation="MNAKLLLKTVFVIIVLLLLVLMGMHNRKPITFTLPPLVKDTISQ
                     PAAIMYFGFFAIGFLTGTVLTAGGGKRGGGGKSKGD"
     gene            complement(1549..>2131)
                     /locus_tag="VG754_04135"
     CDS             complement(1549..>2131)
                     /locus_tag="VG754_04135"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWF18427.1"
                     /translation="GKDPGNIVGLQQEKKYTLLLAQELCDQLRRVGFNASLTRTTDAF
                     IPLPDRPEIARRRAADLFISVHWNELPRNRDMKGAQTFCLTPAGASSSNSGGRSLIGA
                     WRSPGNRNDEKNMLLAYAVQKSLLGNVGVEDRGVRRARYWILRDATMPAILIEGGFMS
                     NPVEAQRIYDAGYRRQMAHAIVEGIRAYQRQVE"
BASE COUNT          459 a          638 c          537 g          497 t
ORIGIN      
        1 gtgatgaaaa ttctcggctc gattttgatc ggcgtggcca ttttgatttt cgtgaccacc
       61 ctcgttctcg tcttcacgtc gccgggttat ttcggctcgc gttacctgcg caagttgatg
      121 gaaactgagt ttcgcaagcg cagcaagcca ttggtcagca ccgccgatgc cgacacgcgt
      181 tttgtgcagg taattccccg cgccaattgg gccaagctca aattgattga cgcgacggac
      241 gttggctttt tgcgcgtcga taagcagcgc ggcgaactgc ttttcgaagg cgacaaggaa
      301 tgttatcgaa ttcccgcggc ggcgatcact tcctgcgaaa ttgaaacgtt catccagggc
      361 cagggctcgc atggagcgac gactctttat cgcgtggtct tgcaagcgaa ccacccctcc
      421 ggattttggg aggcgccgtt cgcccagtgc ggcaacagcg gaaaatatcg cagaaaatca
      481 cgcgaaaaat gggcgctcgc attgcaggga gaaatccgcg aattgatgtt cacggcaaaa
      541 aatcaagcca cgccggtctg aggcgcgcga ttatttctgc gccttcgttt cgacccatcg
      601 cgcaaagagc cacaacaaat ccgcgagacg attgagataa ataatgattt ccggattttg
      661 cagttgacct gcctcatgca aagcacacac acgccgttcc gcgcggcggc aaaccgtccg
      721 cgcaacatcc agtgccgccg aatgaacact cgcgcccgga gtcgcccaac ctttgaacgc
      781 gattttctgc gactcgattt cgacaaccag cttgtccagc ttcgcggtca attccggcat
      841 cacgagcgaa aaaccgtcct tcgcgtaacg cggcaaatct tcgaccatgg tcgccagttc
      901 gcccatgaga atgacgaggt ctttctggat gggcagcaaa ttgtcccgga caaaatcatg
      961 ttccgccgtg gcgcgcgcaa ggccaagcgc cgcgttcaat tcatccacgg aaccataagc
     1021 ttcgacccgc ggatgacact tcgaaacgcg ccgattatac atcaatcccg tcgtgccttt
     1081 gtctcccgtc ttggtggcta tgctcattcc ttttcatttt caccaagagc gggcaaatcg
     1141 caatctcaat tgacggcgct tgcggcattt tctaacttca ggcattgcat cgcctatgaa
     1201 cgccaagctt cttttgaaaa ccgtttttgt gatcatcgtg ctcctgctgc tcgtgctgat
     1261 gggaatgcac aaccgcaagc cgatcacttt cactctgccg ccattggtga aagacaccat
     1321 cagccagccc gcagccatca tgtacttcgg ctttttcgca atcggtttcc tcaccgggac
     1381 tgtccttacc gctggcggcg gcaaacgcgg cggtggtgga aaatccaagg gcgactgact
     1441 gaagcgaata gaagattgag gatagaggat ggcaaaaact gccctgaaaa aatctatcct
     1501 ctatcttcta tcctcagttt gtcttttgcg cctctttgtg gccattcctc attccacctg
     1561 tctttgatag gcccgaattc cctccacaat cgcatgcgcc atctggcggc gataacccgc
     1621 atcgtaaatc cgttgcgcct ccactggatt tgacatgaaa ccgccttcaa tcaagatcgc
     1681 aggcatcgtt gcgtcccgca aaatccaata acgcgcccgg cgtacgccgc ggtcctcaac
     1741 gccgacattg cccaacaaag atttttgcac cgcgtaagcc agcagcatgt ttttttcgtc
     1801 gttgcgattt cccggactgc gccacgcgcc gataaggctc cgtccgccgc tgttgctgga
     1861 actcgcgccc gccggcgtga gacaaaatgt ttgcgcgcct ttcatgtcgc ggtttcgtgg
     1921 caattcgttc caatgaacgc tgatgaacaa atccgccgcg cgacgccgcg cgatttccgg
     1981 cctgtccggc agcggaatga acgcatccgt cgtgcgggtc agcgaagcat tgaatccgac
     2041 gcgccgcaat tggtcgcaca attcctgcgc cagcagcagc gtgtatttct tttcctgctg
     2101 caggccgacg atgtttcccg ggtcttttcc g
//
LOCUS       DASZMM010000170         4536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2542723, whole genome shotgun sequence.
ACCESSION   DASZMM010000170 DASZMM010000000
VERSION     DASZMM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542723"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="VG754_04140"
     CDS             complement(<1..1715)
                     /locus_tag="VG754_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWF18428.1"
                     /translation="MLTQKDPYRLTASLVFFLLFPSLCFGQISIGEAAKAPKLPPAPQ
                     PAIRARTDVTVTGGFNVNTDSREQTRTFYNGIYPTSANVPMYSTADVPNCTPGTNSTA
                     FVEAVLRRINWFRAMAGMPAAITFNPTYNEEAQQMAVMISANHELNHNPPTNWSCYTA
                     EGANASGGNQAEGFNGADAITGYIWDFGVNNNEVGHRRWILLPQTQVMGTGDVPASSD
                     TSTNYAAANSTYVFDSSINSPRPATRKPYVSWPPEGYVPYQVVYPYWSFALSNANVSA
                     ATVTMKSNNVNVAVNLQPQVNGYGENTLVWVPMGLDATQQGTAFPFNGTDTVYSVTVS
                     NIAVVNGNTTNEVSFAYNVTVFDPAVTGTDYVPPVINGPSQPIVGAGNVYSCAAINNP
                     NVTSYEWIISKLIPGNLADDANNGLNNFILSPAPDYSVITNSPDGTGSNCFHLCHDNA
                     TSQFMQVSEVFCPATNSALTFQSLIGFATGDESARVQASTDGGNTWQDLYVQYGCDNG
                     PTNMQCESTFTPHTISLSAFAAQPTLLRFNWAYTGGGYYPETDNYIGWSIKNIVVTNV
                     NQMINT"
     gene            2094..4148
                     /locus_tag="VG754_04145"
     CDS             2094..4148
                     /locus_tag="VG754_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF18429.1"
                     /translation="MKKSQIKNERLNRRSASWPRGMSLGLAILSIASATFAATDETNV
                     MNHLTEISIDDLLNQAVTSVAKKPEQLSKSPAAISVLTQDDIQRSGARSIPEALRLVP
                     GLDVAQVDSQQWAVSARGFNDVFANKLLVMQDGRSLYTPLFSGVFRDVQNPLLEDIDR
                     IEVIRGPGASLWGANAVNGVISIITKSAADTQGLMVLAGGGNYEQDFAAARYGGKLAE
                     DVYYRVDGQYFDRGDSVAAGGNDAHDAWRLGQGGFRIDWDTRKKGGDLLTLQGDVYDG
                     KMEEIYGVFGFPPPAITPMADAESVAGGNILGRWSHEFEEGNDLSVQAYYDRTARETL
                     IFNENLDTFDIDAQHHFTLGDKFRNDIVWGIGYRLSQDHLGNTATVSFNDSSPETQLF
                     SAFVQDEITLVPDRLQLTLGTKLEHNNYTGFEVQPDGRLLWTPAEHHTIWGAVSRAVR
                     TPSEAEEYLRLNQVYPAGVIAPIPVPVTLYGNQNMHSEDLLAFQLGYRVEVCSNFTMD
                     VATFYNIYDDIRSEEPILPFSPFAPVPFRLVNDLHGDSYGLELAPAWQVLDWWRLRPA
                     YSFLETTLKGPDTFTINNDAGGSPEQQFSLRSSMDLPHNVSLDCTLRYVDRLQTPLFR
                     ISSYVALDVRLAWRPSKNWELALVGQNLGSAHHAEFAPTFIGTQRTEVRPGGYAKVTW
                     HF"
     gene            4225..>4536
                     /locus_tag="VG754_04150"
     CDS             4225..>4536
                     /locus_tag="VG754_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HWF18430.1"
                     /translation="MAKVLMLSIFSGLLLAGFAAYADESTPSEYQVKAAFLYNFAKFV
                     KWPESAFAQTNSAIKIGILGEDPFGSNLELAIKGKTINGHPLSVIELSSPDEMKNFQI
                     IF"
BASE COUNT         1059 a         1183 c         1280 g         1014 t
ORIGIN      
        1 gtatttatca tctgattaac attggtgacg acgatgttct tgatgctcca accgatgtaa
       61 ttatcggttt caggataata gccgccaccg gtataagccc aattgaaccg cagcaacgtc
      121 ggctgcgcgg caaatgcgga caacgagatc gtatgcggcg tgaaggtgga ttcgcattgc
      181 atgttggtcg ggccattgtc gcatccgtat tgcacataaa ggtcctgcca tgtgttgccg
      241 ccatcggtgc tggcctgcac gcgggcggat tcatcgcccg tggcaaatcc gatgaggctc
      301 tggaaagtaa gcgcgctgtt ggttgccgga caaaacactt cgctcacttg catgaattgc
      361 gacgtggcgt tgtcatgaca aagatgaaag caattgctgc cggtgccatc aggcgagttg
      421 gtgatgaccg aataatccgg cgcgggcgaa agaataaaat tgttcaagcc gttatttgcg
      481 tcgtcagcca agttgccggg aatgagtttg gaaataatcc attcgtaact ggtgacattg
      541 ggattgttga tggccgcgca actataaaca ttccccgcgc caacaattgg ctgcgatggg
      601 ccgttgataa cggggggaac gtaatcggtt ccagtaactg cgggatcaaa caccgtcaca
      661 ttgtaggcga agctcacttc gttggtggta tttccgttga cgacggcaat gttgctgacg
      721 gtcacgctgt aaacagtgtc tgtaccgttg aaaggaaagg cggtgccctg ctgcgtcgca
      781 tcgaggccca tcggcaccca tacaagagtg ttttcgccgt agccattcac ttgcggttgg
      841 agatttaccg ccacgttgac gttgttgctt ttcatggtga cagtcgcggc gctcacgttt
      901 gcattcgaga gggcgaacga ccaataggga tatacgacct gatacggcac ataaccttcc
      961 ggcggccagg aaacataagg cttgcgcgtg gccggacgtg gactgttgat gctggaatca
     1021 aaaacgtaag tggaattggc ggcagcgtaa ttggtggaag tgtccgaact cgcaggaacg
     1081 tcgccggtcc ccatgacttg cgtttgcggc agcaaaatcc agcggcgatg acccacttcg
     1141 ttattgttca cgccaaaatc ccagatatag cccgtgatgg cgtccgcccc attgaaaccc
     1201 tccgcctgat tgcctcccga ggcgttcgcg ccttctgctg tgtaacaact ccagttcgtc
     1261 gggggattat gattgagctc gtgattggcg gaaatcataa cggccatctg ctgggcttct
     1321 tcgttgtaag tgggattgaa cgtaatggcg gcgggcatcc cagccatggc gcgaaaccaa
     1381 ttgatgcggc gcagcacagc ttccacaaag gcagtcgaat tcgtgccggg cgtgcagttc
     1441 ggcacatccg ccgtggaata catcggcaca ttcgcggagg tgggatagat gccgttgtaa
     1501 aatgtccgcg tttgttcgcg ggaatccgtg ttcacattga aaccgccggt gaccgtgaca
     1561 tccgtgcgcg cacgaatggc tggttgggga gcaggcggaa gtttaggcgc cttggcagcc
     1621 tcgccgatgg aaatctgccc gaaacaaagc gacgggaaga gcaggaagaa aacaaggctg
     1681 gcagtcaatc ggtacggatc tttctgggta agcatgaaga acgacttgaa gtttggaatt
     1741 actgggcaac acgtgttaaa tctccaaaac ctcccagaga aatcaggcaa agtcaaacga
     1801 ccggagacaa attcagcaca gtcacaacaa aatcccgtgt cgtaatcaat tgtcccccat
     1861 ggatagctgc gtctttgttt gagacactcg atatcaaagc tattttcgca ttgccggttg
     1921 cgcaccgtgg acaaatttgt cccatgccgg gacaatgggc tcaaagtcca acgcggcaaa
     1981 gctgccatcc accttccgta acgagcgggc gaatcactga cgctcacttc tctcaattcc
     2041 tacaagtgcg ggcacataag ctgctgaatt gcaaccgcac ctatgggaaa accatgaaaa
     2101 aatcgcaaat aaaaaacgaa cggttgaacc gaaggtccgc ctcgtggccg cgagggatgt
     2161 cgttgggcct cgcaattctt tccatcgcca gcgcgacctt tgcggcgaca gacgaaacca
     2221 acgtcatgaa ccatttgacg gaaatatcca tcgacgacct gctcaaccag gctgtgacct
     2281 cagtggccaa gaagccggaa caactttcca aatcgcctgc cgccatttcc gtgctgacgc
     2341 aagatgacat ccagcgatcc ggcgccagaa gcatccccga agccttgcgg ttggtgccgg
     2401 gactggatgt ggcccaggtg gattcgcagc aatgggcggt gagcgcgcgc ggcttcaacg
     2461 acgtcttcgc aaacaaattg ctggtcatgc aggatgggcg cagcctttac acaccgcttt
     2521 tttccggcgt gttccgggac gtgcagaatc cgttgctgga agacattgat cgcatcgagg
     2581 tcattcgcgg gccgggcgca agtttgtggg gcgcaaacgc cgtgaatggc gtcatcagca
     2641 tcatcacaaa gagcgcggcg gacacgcagg gtttgatggt gctggcgggt ggcggcaatt
     2701 acgagcagga tttcgcggcg gcgcgttacg gcggaaaact tgcggaagat gtctattacc
     2761 gcgtggacgg gcaatatttc gatcgcggtg attcagtcgc tgcgggtggc aacgacgcgc
     2821 acgacgcgtg gcggcttggt caaggtggat tccggattga ttgggacacc cgaaaaaaag
     2881 gtggtgatct gctgacgctg caaggggacg tttatgacgg aaaaatggag gaaatttatg
     2941 gtgtcttcgg tttcccgcct cctgcaataa cacccatggc cgacgcggag agtgttgccg
     3001 gcggaaacat ccttggaaga tggtcgcacg aatttgaaga gggcaatgac ttgagcgtgc
     3061 aagcctatta cgaccgcaca gcccgggaga cactcatctt caacgaaaat ctcgatacgt
     3121 tcgacattga tgcgcaacat catttcaccc tcggcgacaa gtttcgcaac gacattgttt
     3181 ggggcattgg ttatcgcctg agccaagacc atctgggcaa cactgccaca gtttcattta
     3241 atgacagcag cccggaaacc cagttgttca gcgcttttgt gcaggatgaa atcacgcttg
     3301 tgccggaccg tttgcaactg acgctgggca cgaagctgga acataacaat tacacaggct
     3361 tcgaggtcca gccggatggg cggttgctgt ggactccggc ggaacatcat acgatttggg
     3421 gcgcggtttc gcgagcggtg cggacgccat ctgaagccga agaatatctg cggctcaacc
     3481 aagtttatcc agcgggcgtg attgccccga taccggtgcc cgtgacgctt tatggtaatc
     3541 aaaacatgca ctccgaagac ctgctggcct ttcaactcgg ctatcgcgtc gaggtttgca
     3601 gcaacttcac gatggatgtc gccacctttt acaatattta tgatgacatt cgcagcgaag
     3661 agccgatcct tcctttctcc ccttttgccc ccgtgccgtt tcgtctggtg aacgaccttc
     3721 acggagacag ttatggtttg gaattggcgc ccgcctggca ggtgctcgac tggtggcgtt
     3781 tgcgcccggc atacagtttt ttggaaacaa ctttgaaggg accggacaca ttcaccatca
     3841 acaacgatgc gggagggagc cctgaacagc agttcagttt gcgctcaagc atggacttgc
     3901 cgcacaatgt ctccctggat tgcactctgc gttacgtgga ccggctgcag acaccattgt
     3961 tccgcatcag cagttatgtc gcgttggacg ttcggctggc gtggcgtccc agcaaaaatt
     4021 gggaattggc gctggtcggg caaaatcttg gaagtgcgca tcatgcggaa ttcgctccca
     4081 ccttcatcgg cacccaacgg acagaagtcc gccccggcgg ttacgcgaag gtcacatggc
     4141 atttttaacc ggcgcattga atcacttgct cctcctgcga cgtgcaacac tcgggtgcga
     4201 ggctaacagc agatgaaata taaaatggca aaagtcctga tgctcagcat cttttccgga
     4261 ttgctgctgg ccgggtttgc cgcgtacgcc gacgaatcaa ctccttccga ataccaggtc
     4321 aaggcggcgt ttctctataa tttcgccaag tttgtgaaat ggcctgagtc tgcgtttgcc
     4381 cagaccaatt ctgccatcaa aatcggcatc ctcggtgaag accctttcgg gagcaacctc
     4441 gaactggcaa tcaagggcaa gaccatcaat gggcatccgc tcagcgtcat cgagttgtcg
     4501 tccccggatg aaatgaaaaa tttccagatc attttt
//
LOCUS       DASZMM010000171         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4732963, whole genome shotgun sequence.
ACCESSION   DASZMM010000171 DASZMM010000000
VERSION     DASZMM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4732963"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VG754_04155"
     CDS             complement(<1..1219)
                     /locus_tag="VG754_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18431.1"
                     /translation="MAQENDWRGPSSGSWQSPDWSLGILPGANQNIVFTNAGVNLLTI
                     DAATAQNYPQTLNIESLAVSAPYGSTNELLMDAAGLETPLVIGITNEGILSIASGTSI
                     FEMHSSALEVNGGINVSGTFNESDGSEVSAESLGVNGEFNLTNSTLNVAGIETVGQFN
                     QTGGTNTTGGLVLDAVAYPNSQYNMSDGYFAGAITLDYGSFIQTGGQVFASVNFQQYG
                     GKFQFAGGVISQNGLNVQGNPGGPYMQYATSVVFKQTNGTIYSGDLNVGMSGYGEFTQ
                     SGGLLVSSNLNVGVPEEFEWENTPGAPTFFLQSGGVHSNYTITLTGAPLQLVNTPSAS
                     TSRIIVDPNSGKLYGVSTYTFSGGMLYSSAINLSYANFNQTGGTNEVGTISGDSYSLS
                     GGKLIVQNLMETAG"
     gene            complement(1606..>2751)
                     /locus_tag="VG754_04160"
     CDS             complement(1606..>2751)
                     /locus_tag="VG754_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWF18432.1"
                     /translation="LLKFAEAAQIPVTTTIMGCGCFPETHPLSLRWLGMHGSACANWA
                     VSGEFAPKQNDADTPKKIAEGADLLLAFGVRFDDRVTGKVEKFCETGTIVHIDVDPSE
                     INKNKPANLPIVSDIKYALERLNALLKKRGISKKFTAWHKQVEQWKAKAPFSYRVTEE
                     VVTSQHMKDHLTGKENEVILPQHAIELLYELTGGDAIITTGVGQHQMWAGQYYKYKFP
                     RQLLTSAGLGAMGYGYPAALGAKVACPDKQVVDIDGDGSFLMNVQELATAHIEKIAAK
                     ALILNNQHLGMVVQWEDKFYQGNRGHTYLGDPQNMKQIYPDYVAIAKGFNVPCERVMY
                     KKDLRAALQRMLDSDQPYLLDVIVPYTEHVLPFIPAGKTVADMIWKA"
BASE COUNT          575 a          716 c          795 g          665 t
ORIGIN      
        1 cgcctgccgt ctccatcaaa ttttggacaa tcagtttccc gccgctgaga ctgtaactat
       61 cgccagaaat tgttccgacc tcgttcgtcc cacccgtctg gttgaaattg gcataactga
      121 ggtttatcgc agaggaataa agcatcccac cgctgaacgt gtaagtgctg actccataaa
      181 gtttgccgct gttcgggtcc acgatgatgc gggatgtcga agccgatggc gtattgacca
      241 gttgcaacgg cgcgccggtc aaggtgatgg tgtaattgct gtgaactccg ccggactgca
      301 aaaagaatgt gggagcgccg ggggtgtttt cccattcaaa ttcttcggga acgcccacat
      361 tgagattgga cgaaaccaat aatccacccg actgggtgaa ctctccgtat ccggacatgc
      421 cgacattgag gtcgccagaa taaattgttc cgttggtctg tttgaatacg acgctcgttg
      481 cgtattgcat gtacggcccg cccggattgc cctgcacatt caatccgttt tgcgaaatga
      541 caccgcccgc gaattgaaat ttgccgccat actgctgaaa attgacgctg gcgaacacct
      601 ggccgccggt ctgaataaaa ctgccgtaat ccagcgtgat tgcgccggca aaatatccgt
      661 ccgacatatt gtactgagaa ttaggatagg ccacggcgtc caacacgaga ccgcccgtcg
      721 tgttcgttcc gcctgtttga ttaaactgcc caaccgtctc gatgccggca acgttcagtg
      781 tgctgttggt gagattgaac tcgccgttca cgcccaagct ttcagccgac acttccgaac
      841 catcgctttc gttgaacgtg ccgctgacat tgatgccgcc gttcacctcc agcgcggagg
      901 aatgcatctc aaaaatggaa gtgccgctcg cgatgctcag gatgccttcg tttgtgatgc
      961 caatgaccag cggcgtctcc aacccggcgg catccatcaa cagttcgttc gtggaaccgt
     1021 aaggcgcgga gacggcgagc gattcgatgt tgagcgtttg cggataattt tgcgcggtgg
     1081 ctgcgtcaat cgtcagcaga ttcacgccgg cattggtgaa cacgatgttt tggtttgcgc
     1141 ccggcaaaat gccaagcgac caatcaggac tttgccaact gccgctgctg gggcctctcc
     1201 agtcattttc ctgggccatc gctgaaaaac aacaggccaa caatgcaaaa agtacccctg
     1261 tgaatgcgta atttgtacgc cggataagcg agtaggttga cttcatggcg gtgatgctat
     1321 gggtttcgca tcgcgccgca aaatggggtc agctactcca aaagttgagc ctcaccaccg
     1381 taggttttgt acttacaaaa ggcgttttac cgccggagaa aaacttttga acgcaaatgt
     1441 acgcggataa ccgcggcttc aaaattgatg ccaaacgaga ttgacggctc gcgaggtgtt
     1501 tccacactgg caatgctcgt ctcaccaata aaaaattcca agcgcatcgg acgcttggaa
     1561 tttggtgatt ctctggtgat tggatgttgg aatttggagc ttttctcagg ctttccaaat
     1621 catgtcggcg acggttttgc cggcgggaat gaagggcagc acgtgctcgg tgtagggaac
     1681 gatcacgtcg agcaggtaag gctggtcgga atccaacatg cgttgcaagg cggcgcggag
     1741 gtctttcttg tacatcacgc gttcgcaggg gacattgaag cccttggcaa tggcgacgta
     1801 gtcgggatag atctgcttca tgttctgcgg atcaccgaga taggtgtggc cgcggttgcc
     1861 ctggtaaaat ttgtcttccc attgcacaac catgccgagg tgctggttgt tcaggatgag
     1921 cgctttcgcg gcgatttttt ctatgtgcgc ggtggcgagt tcctgcacgt tcatgaggaa
     1981 cgagccgtcg ccgtcaatgt ccacgacttg tttgtcgggg caggcgactt tcgcgccgag
     2041 tgcggcgggg tagccgtaac ccatcgcgcc caaaccggcg ctggtgagga gctggcgcgg
     2101 gaatttgtat ttgtagtatt gcccggccca catctggtgc tggcccacgc cggtggtgat
     2161 gatggcgtcg ccgccggtca attcgtaaag aagttcgatg gcgtgctgcg gcaggatgac
     2221 ttcgttttcc ttgccggtga ggtggtcttt catgtgctgg gaagtgacca cttcctcagt
     2281 gacgcgatag ctgaacgggg ccttggcctt ccactgctca acctgcttgt gccatgcggt
     2341 gaattttttc gagatgccgc gtttcttgag gagcgcgttg aggcgttcga gcgcgtattt
     2401 gatgtcgctg acgatgggca aattggccgg tttgtttttg ttgatctcgg acgggtcaac
     2461 gtctatgtgg acgatggtcc ctgtctcgca aaacttttcc accttgccag tcacgcggtc
     2521 gtcgaaacgc acgccaaagg caagcaagag gtccgcgcct tcggcgattt tctttggggt
     2581 gtcggcgtca ttttgcttgg gggcgaattc gccgctgacg gcccaattcg cgcaagccga
     2641 gccgtgcatg ccaagccagc ggagcgagag cggatgcgtc tcgggaaaac aaccgcagcc
     2701 catgatggtg gtggtcacgg gaatttgagc ggcctcggcg aatttcaaca a
//
LOCUS       DASZMM010000172         5822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4456500, whole genome shotgun sequence.
ACCESSION   DASZMM010000172 DASZMM010000000
VERSION     DASZMM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4456500"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VG754_04165"
     CDS             <1..487
                     /locus_tag="VG754_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF18433.1"
                     /translation="QFDIPANHWRPAIKVSWHQGGAMPESPHRAIDLTKIDHGVMFEG
                     TRGYLVADFQTHLLVPQKNAPGEEADMTYYKPRDKKELIPSPTVFQQEWIDACKGDLK
                     TSCDFGYASDMIEMMLLGLVAYRAGKKIQYDGKTGRVTDSPEANELLSHKYREGWTLN
                     G"
     gene            complement(516..1343)
                     /locus_tag="VG754_04170"
     CDS             complement(516..1343)
                     /locus_tag="VG754_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18434.1"
                     /translation="MVTNQHRAVKKSGFTLIELLVVIAVIGILAALLLPVLASAKRKA
                     HETTCLNNVRQLALTGFMYANENGSPILYNNPRYPDGTWMGSLVDSIKNRKVFICPTA
                     PLHEPAPDSGNRRGASDAAWVRWTSDAREMFFGSYGYNGWLYSDLPKYYPQSTDFVYA
                     KTDGIENTSQTPVFVDANWVDLSPKETDPPWPDLYAGAPFGTSNDDGMGRCTISRHGV
                     SSPSAGPRALTPGQALPGAVMIGFADGHSRLTKLEDLWMLNWHHGWQTPATRPDVGR"
     gene            complement(1354..2736)
                     /locus_tag="VG754_04175"
     CDS             complement(1354..2736)
                     /locus_tag="VG754_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF18435.1"
                     /translation="MTEDATQLRRFVESGDEEAFRALVCRHFGLVYGAALRLTNRDAA
                     LAQDVAQSVFTDLARKAGLLRSDVVLAGWLYQATRFAAAKAVRSEQRRRAREKEAFAM
                     QDRESEPSSPEWDRIGPVLDAAMGRLNAKDRHAVLLHYIEQKNFRSVGAALGISDDAA
                     QKRVSRALDKLRSLLARGGVAVSGSALSTFLSAGTLPSMPASMPLAVAKSSLAASVAM
                     GPPGWTTVFIHNLALVKVRLAMACLLFLLTGGVLTYLFYGAHPVNNGAFVPVDLSAHY
                     NGGLDKSWTPAYGNNYLADLGEGRKVLKRVPFEIHGVVQLQGAEWKRRGYDYPESIEG
                     IRIGMEGRKIHLLHANSAFEDPMGTVVASLIVHYSDGNQTNFSIRQGIEVMDWWEWPR
                     ALKNHPTDTNTLVAWSGSNPAAEHQGARIRLFDTVFINPHPEKEIQSIDYVSGMAGSA
                     PFMVAMTIEH"
     gene            complement(2881..3822)
                     /locus_tag="VG754_04180"
     CDS             complement(2881..3822)
                     /locus_tag="VG754_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18436.1"
                     /translation="MSKLQRLPQSAGFFVIALAAFLSQTSSAQLLSDDFTTDNSLNSG
                     LWTTSSSLLAGLAVEFNSTLIIPTLSFDVSGMNVSGVNGNNELAGVQSLATFHPPFTA
                     TTTVTADEAHGNAYELFLISADLSQWMALSGNLNPGNGSYFGVWVNYDNSGLPFLSLG
                     DNLYSNPSINAQYTIQIFVENTGIATVSLFSSGGILGVQSNLNLGTGPFHLILGQREG
                     GPIVSGQNIATWKNVSLTSLAPAPVLAPMMWTNGMPLISWTAVAGATYQAQYTSALSP
                     AQWNDLGPPITAASSVVTVTDAPASDAQRFYRVVLMP"
     gene            4382..5140
                     /gene="larB"
                     /locus_tag="VG754_04185"
     CDS             4382..5140
                     /gene="larB"
                     /locus_tag="VG754_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWF18437.1"
                     /translation="MTTTEAIKLLEQFRAGGVSRDKVLHAFQAAPVADLGFAHVDTHR
                     ALRKGFPEVIFGAGKTPEQVLKIAAKLLEHEERILITRVTDAHARAVRKKYKRAVHHE
                     IARCITIEKKPMPKRTGHIAVVCAGTSDLPVASEAAVTADIMGNKVESIADVGVAGLH
                     RLFGRLELVQSANVIIVVAGMEGALPSVVAGLVSKPVIAVPTSVGYGASFGGLAALLG
                     MLNSCGSGVTVVNIDNGFGAGYAASQINALVAAA"
     gene            5244..>5822
                     /gene="larC"
                     /locus_tag="VG754_04190"
     CDS             5244..>5822
                     /gene="larC"
                     /locus_tag="VG754_04190"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HWF18438.1"
                     /translation="MKTLYLDIFSGISGDMLLGALIDLGVKPAELGHELKKLALEGYH
                     LHVAPDEKSHIRGIKFDVHVHEHHEHGKAEHSHDDKHEHEHTHDDGVTHTHSHSHGHE
                     HSNEHEHHHHPEQGHEHSHEHEHEDEHHHVHGRNFADIKQLIQKSALSNWVKEKSINV
                     FHRIAVAEGKIHGKPAEQVHFHEVGAVDSIVDI"
BASE COUNT         1417 a         1606 c         1558 g         1241 t
ORIGIN      
        1 ccaatttgac atcccggcca atcactggcg tcccgcaatc aaagtctcat ggcatcaagg
       61 cggcgcaatg ccggaatcgc cgcatcgcgc gattgacctg accaagattg atcacggcgt
      121 gatgttcgaa gggacgcgcg gctatctcgt cgcggatttt cagacgcacc ttcttgttcc
      181 gcaaaaaaat gctcccggcg aagaggccga catgacctat tacaaaccgc gcgacaagaa
      241 ggagttgata ccgtccccca ccgttttcca gcaggaatgg attgatgcct gcaaaggcga
      301 cctcaagacc tcctgcgact tcggttacgc cagcgacatg atagaaatga tgctcctcgg
      361 ccttgtcgct taccgcgccg gcaaaaaaat ccaatacgac ggcaagaccg gccgcgtcac
      421 cgactccccc gaagccaacg aactcctgag ccacaaatat cgcgaaggct ggacgttgaa
      481 tgggtagaag tgctgcacga caataaagat attcattacc gtccgacatc cggccgcgtg
      541 gctggagtct gccacccatg atgccagtta agcatccata aatcttccaa tttggttaag
      601 cgcgaatggc catcagcaaa gccgatcatg acggcccccg gcaatgcctg gccgggcgtc
      661 aacgcgcgcg gaccggctga agggctggac acaccgtgcc gcgaaatggt gcagcggccc
      721 atcccatcat cattgctggt tccgaaggga gcgccggcat aaagatcagg ccatggcgga
      781 tcggtttcct tgggcgacag gtccacccaa ttcgcgtcaa caaaaaccgg cgtttgcgag
      841 gtgttttcga tgccatcggt tttggcataa acgaaatcgg tggactgcgg ataatatttg
      901 ggcaggtcgg aatatagcca gccgttatat ccgtagcttc cgaagaacat ctcgcgggca
      961 tctgaagtcc atcgcaccca cgctgcatcc gatgcaccac ggcggtttcc agaatcaggc
     1021 gccggttcgt gcaaaggagc ggttggacaa atgaatactt ttcgattttt aatggaatcc
     1081 acgaggctgc ccatccaggt gccgtccggg taacggggat tattgtaaag aatcgggctg
     1141 ccattctcat tcgcgtacat gaagccggtg agagcaagtt gccggacatt attgaggcaa
     1201 gtcgtttcat gggcctttcg cttcgcggaa gccaatactg gaagaagcaa tgccgccaga
     1261 attccaatga ccgcgatgac gaccagtaat tcaatcaatg tgaatcccga ttttttaacg
     1321 gcgcgatgtt ggttggtcac cattggggtt gattcagtgt tcaatcgtca tcgccaccat
     1381 gaacggcgcg gagccggcca tgcccgatac atagtcgatg gattgtattt ccttttcggg
     1441 atgtggattt atgaagaccg tgtcgaaaag gcggatgcgc gcgccctgat gttcagccgc
     1501 cggattgctg ccgctccatg cgacaagcgt gttcgtatca gtcgggtgat ttttcagggc
     1561 gcgcggccat tcccaccaat ccatcacctc tattccctgg cgtatggaaa aattggtttg
     1621 gtttccgtcc gaataatgca cgatcaagct cgccacaacc gttcccatcg ggtcctcaaa
     1681 tgcggaattg gcgtgcaaca ggtgaatctt gcggccttcc attccaatgc ggattccctc
     1741 aatgctctcc gggtaatcgt atccgcgccg tttccattcg gctccctgaa gttgaacaac
     1801 gccgtggatc tcaaacggaa cccgtttcaa cacctttcgc ccttcgccca aatcagccag
     1861 gtaattgttg ccataggcag gagtccagct tttatcaagc ccgccattgt aatgcgccga
     1921 caaatccaca ggaacaaacg ctccattgtt caccggatgc gcaccgtaaa aaagataagt
     1981 gagaacaccg ccagtcaaca aaaacaaaag acatgccatt gccagcctga ctttgaccag
     2041 cgcgagattg tgaatgaaca ccgtggtcca gcctggcggc cccatcgcca ccgacgcggc
     2101 cagagaggat tttgccactg ccaatggcat ggaggcgggc atcgatggca gcgtgccggc
     2161 gctcaagaaa gttgataaag cagatcctga aacggccaca ccgccccgcg cgagcaggct
     2221 gcgcaacttg tccaatgccc ggctcacccg cttttgcgcg gcgtcgtcac tgatgcccaa
     2281 ggcggcgccg acggagcgga agttcttttg ctcgatgtaa tggagcaata ctgcgtggcg
     2341 gtctttggca ttcaacctgc ccatcgcggc gtccaacact ggaccgatcc gatcccattc
     2401 gggtgatgat ggttccgatt cccggtcttg catggcaaaa gcctcctttt cgcgagcacg
     2461 ccgcctctgc tcgcttcgca ccgctttggc ggcggcaaac cgagtggcct gataaagcca
     2521 gcctgccaga acaacatcac tccgcaacaa cccggccttc cgggccaaat cggtgaacac
     2581 cgattgtgcg acatcttgcg caagagcggc atcacggttt gtcaaacgga gcgcggcccc
     2641 atataccaaa ccgaaatgcc ggcataccag ggcgcgaaaa gcttcttcgt ctccgctttc
     2701 aacgaaccgg cgcaactgtg tcgcatcttc tgtcatgctc gctttgtctt atagcaccca
     2761 ctccataaga aatccgacat gtattgttgc ctgatggaaa tcgctgaaca acgaaagtcg
     2821 caccttgcat tggaagtcgc acgcctgaaa ttaaccattt cgcgctggcc cctggctttt
     2881 tcacggcatc aggacaacgc gatagaaacg ttgcgcatcg gaggctggcg cgtcggtgac
     2941 agtcacgact gaactcgcgg ctgtgatggg cggtcccaag tcgttccatt gggctggcga
     3001 gagggcgctg gtgtattgcg cctggtaggt ggccccagcc acggcggtcc aggaaatcag
     3061 cggcattcca tttgtccaca tcattggggc aagcaccggc gcgggcgcca agctggtcaa
     3121 agaaacattt ttccaggtgg cgatattttg gccggacaca atgggtcctc cctcgcgctg
     3181 tccgaggatg agatggaaag ggccggtgcc gaggttcaaa ttggattgaa cgccgaggat
     3241 gccaccggaa gaaaacaatg aaaccgtcgc gattccggtg ttctcgacga atatctgtat
     3301 ggtgtattgg gcgttgatgc tggggttgga ataaagattg tcacccaaac tgaggaaagg
     3361 caaaccactg ttgtcgtaat tcacccaaac accgaagtac gagccgttgc cgggattcag
     3421 attgccggac agagccatcc attgcgagag gtccgcgctg atgagaaaca actcataagc
     3481 attgccatgc gcctcatcgg cagtgaccgt cgtggtcgcc gtgaacggcg gatggaaggt
     3541 ggcgagcgat tgaaccccgg cgagttcatt gttgccgtta acgccggaaa cattcattcc
     3601 tgatacgtca aaactcaatg ttggtatgat caatgtggag ttgaattcca ccgccaaacc
     3661 tgccagcagg ctggacgagg tcgtccacaa cccggagttc aagctgttgt cggtggtaaa
     3721 atcatccgag agcaattgcg cggaactggt ttgggacaaa aaggcggcta gggcaatgac
     3781 gaaaaagccg gctgattgcg gcagtcgttg gagcttggac attttttgga ctgcgtccgg
     3841 aaggaccgct cttattttcg ttgaaataac aatcataccg attcttattc cagcacccgc
     3901 caaataagaa atccgacatg aaatggttgc cgcaccacca tctcacttgt ttacgtcgcc
     3961 acgtgaaaaa ggatgccata aaaatcctcg acaaacagac atcgggtctg cataatcctc
     4021 ggcgtgtgga acaaaggagc acacaggcac cccaaaggtg ttcgccatcc ggcttttcag
     4081 ccggaaggaa atttttttat aaaacgtatg cgatcaaggc cgcaacccaa tactccacca
     4141 ctccaatact ccaattccgc gctcatgcgt aagtggttga attcccggat aatttcagaa
     4201 aattccagat agtttcagat aattccgcca aaaacacccc cctccccccc ttgcaaacca
     4261 aaacatcgaa cgctgaactc aaaacagcga ggaacgaact tttcccgcaa cacggaacac
     4321 gcaatggccg acttccattt tcctgcgtga ccatgcgcct tttccctgac attcttgccg
     4381 tgtgacaaca acggaagcca tcaaactgct cgaacaattt cgcgccggtg gtgtgagccg
     4441 ggacaaggtg cttcatgctt ttcaggcggc gccggtggct gaccttggct ttgcgcacgt
     4501 ggacacgcac cgggcgcttc gcaaaggctt tccagaggtg attttcggcg cgggcaagac
     4561 accggaacag gtgctcaaaa tcgctgccaa actgcttgaa catgaggaac gcatcctcat
     4621 cacccgcgtc acggacgccc atgcgcgcgc ggtgcgcaag aaatacaaac gcgcggtcca
     4681 tcatgaaatt gcccgctgca tcacgattga aaaaaagcca atgcccaaac gcacgggtca
     4741 catcgcagtt gtttgcgccg ggacaagcga cctgcctgtc gcctccgaag ctgctgtcac
     4801 cgcagacatc atgggcaaca aggttgaaag cattgccgat gtgggtgttg ccgggttgca
     4861 tcggttgttc gggcggctgg aattggttca aagcgcaaat gtgatcattg tcgttgccgg
     4921 gatggaaggc gctttaccga gtgtggtggc ggggctggtt tccaaaccgg tcattgcggt
     4981 gcccacaagc gtgggatatg gcgcgagttt cggcggactt gcggcgttgc tgggcatgtt
     5041 gaatagttgc ggcagcggtg tgactgtggt caacatcgac aacggctttg gcgcgggtta
     5101 cgccgccagc cagatcaacg cgctggttgc ggctgcgtaa aaaattatgg gcggcatatg
     5161 gcaattgcgg caggtcgttt ggtgtccatg ctttagcatg tattctctgg agcagcctga
     5221 aggctggacg ccaagcaatt aaaatgaaaa ccttgtatct ggatattttc agcggcatca
     5281 gcggcgacat gctgcttggg gcgcttatag acctcggcgt caagcctgcc gaactcggtc
     5341 acgaactgaa aaagctcgcg ctcgaaggtt atcatctgca tgtcgctccc gacgagaaat
     5401 cgcacattcg cggcatcaaa tttgatgtcc acgttcacga acatcacgaa catgggaaag
     5461 ctgaacactc gcatgatgac aaacatgaac atgagcatac tcatgatgat ggcgtgactc
     5521 atacgcattc acactctcat gggcacgagc attccaatga gcatgaacat catcaccatc
     5581 ctgaacaagg gcatgaacat tctcacgagc atgaacatga agatgagcac caccatgtgc
     5641 atggccgaaa ttttgccgac atcaaacaac tcatccaaaa gagcgcgctt tcaaactggg
     5701 tgaaggaaaa atcgattaat gtctttcatc gcattgccgt tgcggaaggg aaaatccatg
     5761 gcaagccggc ggaacaagtg cattttcacg aagtcggcgc ggtggattcg attgtggaca
     5821 tc
//
LOCUS       DASZMM010000173         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_620198, whole genome shotgun sequence.
ACCESSION   DASZMM010000173 DASZMM010000000
VERSION     DASZMM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620198"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VG754_04195"
     CDS             <1..689
                     /locus_tag="VG754_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWF18439.1"
                     /translation="QTDDPSVAAHFLNLLQGKPADDLHVRAMDCSLILYAEHEFNAST
                     FATRITTSTLADFYSAITSGIGTLRGALHGGANEEAMALVEKFKTPDEAETGLMEMLA
                     KKELIMGFGHRVYKISDPRSASIKAWAKKLALTADQQKLYAIFERIESVMMREKKLFP
                     NLDFYSAAAYHFSRIPTPMFTPLFVISRISGWSAHVMEQRANNRLIRPTAEYIGPAPR
                     PYVPVEKRAP"
     gene            686..1429
                     /locus_tag="VG754_04200"
     CDS             686..1429
                     /locus_tag="VG754_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18440.1"
                     /translation="MSQNISLVVIILLAPVPLLVAWWMSVRKRKREAAGPIEDTARQK
                     AERKDFEKKYWVRILIARIVVSIGSLFFLAPIYFLFDDKANLNWKEAAGMAGFGAVLL
                     AFAISLIKNTDMPTAVHLNRAAFFVYVLFFGIMLWRLRLDNLPMVVLVVLVLLLLATA
                     GLICSFSRALPARWILAALQIGIAAFFAFLIFRSRGPALWGHPVAVGLWAAVTCLLVI
                     SLFGTESNIYYAIQVPQQSGQAEINQNHP"
     gene            1532..2023
                     /locus_tag="VG754_04205"
     CDS             1532..2023
                     /locus_tag="VG754_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF18441.1"
                     /translation="MRPSIRQAIPPDTPTIVGILSEAARWLEQTGNIMWRDNELLPSS
                     IAVDVNAGSFFLAECDGEAAGVIKFQLEDALFWPDVPQGESAFVHRLAVRRKFAGGGI
                     SSIILNWAVEQARSLNKKYLRLDCEASRTKLRAVYERFGFVHHSDRQVGPYFVSRYEY
                     KLS"
     gene            2145..>2462
                     /locus_tag="VG754_04210"
     CDS             2145..>2462
                     /locus_tag="VG754_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18442.1"
                     /translation="MKISWRHLLGACAGSACLILASGCVVDSNGRVAFQPLVVVAPPP
                     PIVVAPAPGPVVVEAPMVPDAYVWDGFEYVGFVGNQCFYLGPGNIWLVCDPFRLDRFH
                     GWER"
BASE COUNT          523 a          678 c          635 g          626 t
ORIGIN      
        1 cgcagaccga cgatcccagc gtcgccgcgc atttcctgaa tctgctgcaa ggtaaacccg
       61 ccgacgacct tcacgttcgc gcgatggatt gctcgctgat tctatacgcc gaacacgaat
      121 tcaacgcctc cacctttgcc acacgcatca cgacatcgac gctcgccgat ttttattccg
      181 cgatcacctc cggcatcggc acgttgcgcg gcgcgctcca cggcggagcg aacgaagaag
      241 ccatggcact cgtcgaaaaa ttcaagacgc ccgacgaagc cgaaaccggc ctcatggaaa
      301 tgctcgccaa aaaagaactc atcatgggct ttggccatcg cgtctataaa atctccgacc
      361 cgcgctccgc ctccatcaaa gcctgggcca aaaaactcgc gctcacggcc gaccagcaga
      421 aactttacgc aatcttcgaa cgcatcgaat ccgtgatgat gcgcgagaag aaacttttcc
      481 caaacctcga tttctacagc gccgccgcgt atcacttctc ccgcatcccg acgcccatgt
      541 tcacgccgct gtttgtcatc tcacgcatca gtggctggtc cgcgcacgtg atggaacaac
      601 gcgccaacaa ccgcctcatc cgccccaccg cggaatatat cggtcccgcg ccgaggccgt
      661 atgtgccagt cgaaaaacga gcaccatgag ccaaaatatc agtcttgttg tcatcattct
      721 tctggcccct gttccgttat tggttgcttg gtggatgagc gtccgcaaac gaaagaggga
      781 agccgcggga ccaattgaag ataccgccag gcagaaagcg gagcgaaagg actttgagaa
      841 aaaatattgg gttcggattt tgatcgcacg gattgttgtg tcgatcggat ctcttttttt
      901 ccttgcgccc atctacttcc tattcgacga caaggcgaat ttaaactgga aggaggcggc
      961 aggaatggcg ggtttcggcg cagtgctgct cgcctttgcg atctcgctta tcaagaatac
     1021 cgacatgccc acggcggtac atttaaacag ggccgccttt tttgtttatg tcctgttctt
     1081 tgggatcatg ttatggcgtt tgcggctgga caatttgccc atggtcgtct tggttgtcct
     1141 tgtattgctt cttttggcca ccgcaggcct gatatgttcg tttagccgcg cattgcctgc
     1201 acgatggatt ctagcagcgc tccaaattgg aatcgcggcg tttttcgcat ttttaatttt
     1261 tcgctcgcgg ggtccggctt tgtgggggca tccagttgcg gtgggattat gggcagccgt
     1321 tacatgcttg ctcgtcattt cgctttttgg aacagagagt aatatctatt atgccatcca
     1381 agtccctcaa caaagcggcc aagccgaaat aaatcagaac catccgtgac acggccattt
     1441 ccaaattctt tgggaaagcg gccgacccga cctcgttgag gttggtatca gcttgagacg
     1501 attaggtttt cctctaaatt gttttcaaat catgcgtccc tctatccgcc aagcaatacc
     1561 gccagacacg ccaaccattg tcggcattct ctctgaggcg gcgcggtggt tggagcaaac
     1621 cggcaacatc atgtggcgcg acaacgaatt gcttccttcc agcatcgccg ttgacgtcaa
     1681 cgccggatcg ttcttcctcg cggaatgcga cggcgaagct gccggggtca tcaaatttca
     1741 acttgaggat gctttgttct ggcctgacgt gccgcaaggt gaatcggcat ttgtgcatcg
     1801 acttgcggtc aggaggaaat ttgcaggcgg cggcatttca tcaataattt tgaattgggc
     1861 agtcgaacag gcgcgttcgt taaacaaaaa atatctgcgt ctggattgcg aggcctcccg
     1921 gacaaaattg cgcgcggttt atgaacgctt tggttttgtc catcacagcg acaggcaagt
     1981 cggcccctac tttgtttcgc gttatgaata taagctttca taacccgtgt ttctgaggtt
     2041 aagccttgct gccaatcctt tcccccggta aactcattgc cagttaattt tgaatgccgg
     2101 ggtgcgattc atctcaagcc gccagcaatt tagcaaaaaa atatatgaaa atatcatggc
     2161 ggcatttgtt gggcgcgtgt gcgggttcag cctgtttgat tttggcatcc ggttgtgtgg
     2221 tcgattccaa cggcagagtc gcgtttcaac ctttggttgt cgtagcgccc ccgccgccga
     2281 tagtcgttgc tcccgcgccc gggcccgttg tggtcgaggc cccgatggtt cctgatgctt
     2341 acgtgtggga tggcttcgaa tacgtgggtt tcgttggcaa tcaatgtttc tatctcggcc
     2401 ccggcaatat ctggctggtt tgcgatccgt tccggttgga ccgttttcac ggatgggaac
     2461 gg
//
LOCUS       DASZMM010000174         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1980065, whole genome shotgun sequence.
ACCESSION   DASZMM010000174 DASZMM010000000
VERSION     DASZMM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980065"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="VG754_04215"
     CDS             complement(<1..1763)
                     /locus_tag="VG754_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18443.1"
                     /translation="MPFLLLVAVEVCLRLAGYGYNPHFFKRISIGGADFFVQNEDFSL
                     TFFPKKMVRNPGPLRFPVHKPPGTFRIFILGESAAMGDPAESFAPDRYLEELLRQKYP
                     ERKFEVINTAVTAINSHVILPIARECAAREGDLWIIYMGNNEMVGPYGAATVFGRQAP
                     PLAFARLATASQKLRIGQWLKAMEQKFAGRNREKAAWGGMEMFLGNQIPPDSPVRETV
                     YQNFQKNLDDIIRAGINSGATVLLNTVGVNLRDCPPFGSLPGRSLAPAERAEFETLYT
                     NSQQAAAQHQWAAAETGFAQAARLDDRPAEFQYRWAECMLAQTNLAEARKHFQLACDC
                     DALPFRADSRINAAIRDETRRIKSNRLIFCDAAKALADGNAAGICGDEIFFEHVHFDF
                     DARYRLARAWAEQIESLFPRTTNGWISQAECEKRLGLSPWNRSQVIHLMVERMQVPPL
                     SSQANNEERRRALESRIKSELAKMNHDQADRTRQNFEQHLAQRPDDFFLRENYAVFLE
                     LSGDVPAASTQWERFRDLLPQDPLGYFEAGRLLIPQQHYAEAETLLRQTLAIRPIRTD
                     GWIELGNALALQKKYTEALDC"
     gene            complement(1973..2848)
                     /locus_tag="VG754_04220"
     CDS             complement(1973..2848)
                     /locus_tag="VG754_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18444.1"
                     /translation="MKIKYRQTNLNGLGFLSLRRAFTLIELLVVIAIIAILASLLLPA
                     LSQAKQKAVAAACMSDKRQISLAWVMFAGDNNDLLPYNNDFSFSGSLTFNGQPSWAGG
                     KIDWTQGLYNTNETLVTSDSYASLGPYVSQSVKIFACPAANFVSTVQRAQGWSHRIRS
                     IAMNAAVGDGVKYNSPSPFGWTQWYVAKKSTDFHFPGPSDCWVFTDEHPDSIDDTILY
                     TASYPVTSFTELPGNQHGGRCGLTFGDGHAEIHSWKGPVMSAHQNVIYQTVQQVSCSK
                     TDEDMNWLAIHTPQN"
     gene            3099..>4623
                     /locus_tag="VG754_04225"
     CDS             3099..>4623
                     /locus_tag="VG754_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWF18445.1"
                     /translation="MKRIAKICGLTAMLTMAFMFAFPACAVVLWSNYDPITVTTNGPG
                     ADLLGGAVKRDDAANDTLYFKLRVEPISDETTEPYFAALELFEDDVERLGVGNALDAW
                     AYSVFFPGAQSSGVPPAGYLDLRSAHPDSTAVARLANYQCPRRGETSTIVFKIQYVPN
                     GEDLVTVWLNPDLGPGANEVNQPEALTTHFNVNASFDELRLRHGGNGGGWKFSDLAIA
                     TAFHDFADSSSTQPGSGEADLAQSKDSLQFQSWLKNQGLPRVTVDAIQQTSDGYLWIA
                     AGGSIARFDGVRFVPLNAHVSETNFSTQTIFGDSQGALWVAVSNGLQRWENGHATMLT
                     LNDGLPNTHITALNEDGQGNIWIGTTSGLAIWNNGRLVPLADAKRIRGRAITAIFRDR
                     KDSMWLVVQGTEVFQFDHDHFSPLTTDSAKDWMTDIHALLVDAMGRVWLATGEDAVLC
                     RDKDGWRHYRIPKRTTGSRVNAIAEEPDGTVWAGGAGGLFLFSNGKFSAISASSRLAG
                     "
BASE COUNT         1036 a         1273 c         1251 g         1063 t
ORIGIN      
        1 aaacaatcca gcgcctcggt gtattttttc tgcaacgcca gagcgttgcc cagttcgatc
       61 caaccatccg tgcggatggg ccggatggcc aaagtttgcc gcaacaatgt ttcggcttcc
      121 gcgtaatgct gctgggggat cagcagccgc ccggcttcaa aataacccag cgggtcctgc
      181 ggcagcaaat cacggaagcg ctcccattgc gtcgaagccg ccgggacatc gccggacagc
      241 tctaaaaata ccgcgtaatt ctctcgcaga aagaaatcat ccgggcgttg cgccagatgt
      301 tgttcaaaat tttgacgcgt ccgatcggcc tggtcgtgat tcatcttcgc aagttcggac
      361 ttgatgcggg attccaaggc gcgccggcgt tcctcgttgt tggcctgact gctcaacggc
      421 ggaacttgca tccgttccac catcaagtgg atgacctgcg agcggttcca cggcgaaagc
      481 cccagtcgtt tctcgcattc cgcctgcgaa atccagccgt tagtcgtgcg cggaaacaac
      541 gattcgattt gctccgccca ggcgcgggcc agccggtagc gggcatcgaa atcaaaatga
      601 acatgttcga aaaatatttc atcgccgcaa atgccggcgg cattgccgtc ggccagcgct
      661 ttggctgcat cgcaaaagat gagacgattg ctttttatcc tgcgcgtttc gtcccgaatg
      721 gcagcgttga tccgcgaatc cgcccggaaa ggcagggcgt cgcagtcgca ggccagttga
      781 aaatgctttc tggcctctgc gagattggtt tgcgcgagca tacactcggc ccatcggtat
      841 tggaattcag cgggccggtc gtcgagccgt gccgcctgag caaagccggt ttcagcggcg
      901 gcccattgat gctgtgcggc ggcttgttgg ctgtttgtgt aaagtgtctc gaactctgcg
      961 cgctcagccg gggccaagct gcgtcccggc aatgagccaa acggcggaca atcccgcaga
     1021 tttacaccca ctgtgttcag caaaaccgtc gcgcccgaat ttattccggc gcgaataatg
     1081 tcatccaagt ttttttgaaa attttgataa actgtttccc gcacgggact gtccggggga
     1141 atctggttgc ccagaaacat ttccatgccg ccccaagcgg ctttttcccg gttgcgcccc
     1201 gcaaatttct gctccattgc cttcaaccat tggccaatgc gcaatttctg cgacgccgtt
     1261 gccaaccgcg cgaaggccaa cggtggcgcc tgccggccaa acactgtcgc cgcgccatac
     1321 gggccgacca tctcattgtt gcccatgtaa atgatccata gatcgccctc gcgggccgcg
     1381 cattccctgg ctatgggcag gatgacatgg gaattgatgg cggtaacagc ggtattgatg
     1441 acttcaaact ttctttccgg atacttttga cgcaacagct cttcaagata gcggtccggc
     1501 gcaaaactct cggcgggatc tcccatggca gcggattcgc ccaaaataaa gatgcggaat
     1561 gttcccggag gcttgtggac tggaaaacgc aacgggccgg gattgcgcac catttttttg
     1621 gggaaaaaag tcaaactgaa gtcctcgttc tgcacaaaga aatccgcccc cccgatcgag
     1681 atgcgtttga aaaaatgcgg gttgtaaccg tagccggcca gtcgcaggca aacttcaacc
     1741 gcgaccaata gcaaaaaagg catcagcacc aacacgaatc gaaacacccg acgccgaccg
     1801 catgaaagcg gcttgtggat tgtgggcgaa ccggctgagg ccggacgttt gttttttttg
     1861 cccgctttca agctggactg ctgttcttat ccgaagcggg gcaagactcc accgctggcc
     1921 cgattaatct atcggtgcca gccaatcctg cagagctcgg tgacaccaac ttttaattct
     1981 gtggcgtatg aattgccagc caattcatgt cctcatccgt tttcgaacaa gaaacttgtt
     2041 ggacagtctg gtaaatcaca ttttgatgag cagacatgac cggtcccttc caactatgga
     2101 tttcagcatg gccatcgcca aaggtcaaac cacaccgccc gccatgctgg tttccgggga
     2161 gttcggtgaa actggtgacc ggatatgagg cggtgtaaag aatcgtatca tcgatgctgt
     2221 caggatgctc gtcggtaaaa acccagcaat cagaggggcc gggaaagtga aaatcagtgg
     2281 attttttcgc cacataccat tgtgtccagc caaacggcga cggagagttg tatttgactc
     2341 cgtcacccac ggcggcattc atggcaatgc ttcgaatccg atgactccag ccttgggcgc
     2401 gttgtactgt acttacgaaa ttagccgccg ggcaggcaaa aattttgacg gactgggaaa
     2461 cataagggcc gagtgatgcg tatgaatcgc tggtgaccaa agtctcgttg gtattatata
     2521 gaccttgggt ccaatcaatc ttgccgcccg cccatgacgg ttgaccattg aaagtaagcg
     2581 aaccgctgaa cgagaagtca ttattgtaag gcagcagatc gttattgtct cccgcgaaca
     2641 ttacccacgc aagtgagatc tgtcttttat cgctcataca agccgcggcc acggcctttt
     2701 gcttggcttg acttaaagcg ggcagcagca gagaggcaag gatggcgata atggctatca
     2761 ccacaagcag ttcaatcagc gtgaaggccc gtctcagcga cagaaatccg aggccgttaa
     2821 ggttggtttg acgatatttt attttcatgt tgggacagaa aactgatcca tcaccttccg
     2881 ggttttacca tcaggttgcc tgtctccaag agacgcggat agatacagca gttcataaac
     2941 tagtcttgcg ggctgttttg cctaaaattt agcgcggcgt tgatttcccg acagtcgcat
     3001 gaagtggcga caggcttgcc tgctctcgcg gcgatcgcag ttaattcatt tttttatcgt
     3061 aactatgaaa gttttttgcc attttcaaag agaagcccat gaaacgcatc gcaaaaatct
     3121 gcggcttaac tgccatgttg acgatggcat tcatgttcgc ttttccggct tgtgcagtcg
     3181 ttttgtggag caattatgat cccataactg tcaccaccaa cggccccggc gccgacctgc
     3241 ttggcggagc tgtaaaaagg gacgatgctg cgaacgacac gctgtacttt aaattgcgcg
     3301 tcgaacccat ttctgacgaa accaccgagc cttacttcgc ggcgttggaa cttttcgaag
     3361 acgatgtcga acgtctcggc gtgggcaatg ccctggacgc ctgggcttac agcgtctttt
     3421 ttccgggagc gcaaagcagc ggtgtgcccc ctgccggata cctcgacctg cgatccgccc
     3481 accccgattc cactgccgtc gcgcgtcttg ccaattacca atgtccccgc cgtggcgaga
     3541 cttccaccat tgtctttaaa atccagtacg ttcccaacgg cgaagacttg gtcaccgtct
     3601 ggctcaatcc cgacctcggc cccggcgcga acgaagtgaa tcaacctgaa gcgttgacca
     3661 ctcactttaa cgtgaatgct tctttcgacg aactccgtct ccgtcacggc ggcaatggtg
     3721 gtggctggaa atttagcgac ctggcgattg ccaccgcgtt ccatgatttt gcggattcga
     3781 gcagcaccca acccggcagc ggcgaagcgg acctggccca aagcaaagac tccctgcaat
     3841 ttcaatcctg gctgaagaac caaggattgc cccgcgtgac agtggacgcc atccagcaaa
     3901 cgagtgacgg ctatctctgg attgccgctg gcggctccat tgcgcgtttt gatggtgtga
     3961 gattcgttcc gttgaacgcg catgtctcgg aaacgaattt ttccacccaa accattttcg
     4021 gcgacagtca gggcgcgctg tgggtggccg tttcaaatgg tttgcaacgc tgggaaaacg
     4081 ggcatgcaac catgttgact ttgaatgatg gtttgcccaa cacccatatc accgccttga
     4141 acgaggacgg gcagggtaat atttggattg gcacgacgag cgggttggca atttggaaca
     4201 atgggcggct tgttccgctg gcggatgcga aaaggattcg cggccgcgct atcactgcca
     4261 ttttcaggga ccgaaaggat tcgatgtggc tggtggtgca aggaacggaa gtttttcagt
     4321 tcgaccatga ccatttctcg cccctcacca ccgattcagc caaagattgg atgacggaca
     4381 tccatgcatt gctcgtggac gccatgggca gagtctggct ggccaccggc gaggacgctg
     4441 tcttatgccg cgacaaggat ggctggcgcc attatcgcat tccgaaacgg accacgggtt
     4501 ctcgagtgaa cgcaatcgca gaggaacctg acggcacggt ttgggccggt ggcgccggcg
     4561 ggttgttttt gttttcaaac ggtaaatttt ccgccatttc tgccagcagc agactcgcgg
     4621 gga
//
LOCUS       DASZMM010000175         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_64574, whole genome shotgun sequence.
ACCESSION   DASZMM010000175 DASZMM010000000
VERSION     DASZMM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64574"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VG754_04230"
     CDS             <1..432
                     /locus_tag="VG754_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HWF18446.1"
                     /translation="ATFTLRLAFGTVKGYEEDKKWIASFTTFSGLYEHAKEMKNRPPF
                     DLPEIWVKRKSRLNLDTPFNFVSTDDIIGGNSGSPVVNRAGEFVGIIFDGNLQSLPWD
                     YAYTDKQSRVTSVDSASIVEALDRVYDAKDIAAELVSGHRK"
     gene            579..655
                     /locus_tag="VG754_04235"
     tRNA            579..655
                     /locus_tag="VG754_04235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Arg,seq:ccg)
     gene            674..1324
                     /locus_tag="VG754_04240"
     CDS             674..1324
                     /locus_tag="VG754_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18447.1"
                     /translation="MKSFLPWLLVVALIAGVYFLYAQVKDRDAQVAKLSQDNLEVAQL
                     RAENEELKKIPLQTQELDRLRKEHDELLRLRNETQRTKEQVKQLTAQLAAAQAQTAAV
                     QAQNEKAQQIEQQATQLANENQDLRKQADRATSEEKVAQQQIATCANNLRIIDAAKQQ
                     WALENKKTATDIPTPEDIAPYLPNPAQIVCPAGGVYSINAINTAPTCSVPSHNLPK"
     gene            1464..3302
                     /locus_tag="VG754_04245"
     CDS             1464..3302
                     /locus_tag="VG754_04245"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWF18448.1"
                     /translation="MNTSIEEALSGIANQLRIDSIMATTAAGSGHPSSCCSAADLVAA
                     LFFGHMRFDPKNPKYPNNDRFILSKGHAAPLLYAAWAELEFIPREHLLTLRRLDSDLE
                     GHPTPRLPFVDVATGSLGQGLGVGAGMALCARLDKLDYRTYVLMGDGECAEGSVWEAA
                     SLAGIYQLNNLVAIVDVNRLGQSEPTALGYDVDAYRKRFEAFNWRTEVINGHDMEEIL
                     EVLGAAGLGKQPLAIIAKTQKGHGVSFMEDKEGWHGKALSKEEAETAIAELQPKARTG
                     LPEPIPAPSELPLPKNEAPSSYPALNYKLGDSIATREAYGNALLRIGSVDERIVAVDG
                     DTKNSTFADKFFKKFPERSTECYIAEQNMVGVATGYATRGKVPFASTFACFFSRAYDQ
                     IRMAGISMANVKLVGSHVGVSIGEDGTSQMALEDIAMMRAVAGSVVLYPSDAVSAEKL
                     VEQMALHKGVSFLRTSRPKTPVIYGNDESFPIGGAKIRKQGAGDKVTVVGAGVTLFEA
                     LKAADELAKDGIGITVIDAYSVKPLGKDVIKDAAQKTGNLVVTVEDHYAEGGLGDAVA
                     GELSEDGIKVHKLAVRELPHSGKPEELLAKYGIDSAAIVKAVKSLV"
     gene            3378..>3600
                     /locus_tag="VG754_04250"
     CDS             3378..>3600
                     /locus_tag="VG754_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18449.1"
                     /translation="MSGHATPKLRLLAVFLIFFVSAGALLAQPLVCAICGKPIYGNYY
                     VNEDQIEHVQKNVCSNCNAVEARCYYCQLP"
BASE COUNT          852 a          946 c         1059 g          743 t
ORIGIN      
        1 gcgacgttca cgctgcgcct ggcgttcggg acggtgaagg gttacgagga agacaaaaaa
       61 tggatcgcgt cgttcacgac tttcagcgga ttgtatgaac acgcgaaaga gatgaaaaat
      121 cgtccgccgt ttgatttgcc ggaaatttgg gtgaaacgaa aatcgcggct gaatttggat
      181 acgccgttca attttgtgag cacggacgat atcattggcg gcaattccgg gagtccggtg
      241 gtgaaccgcg cgggcgaatt tgtgggcatc atctttgacg ggaacttgca aagcctgccg
      301 tgggattacg cgtacacgga caagcaaagc cgtgtgacct cagtggactc ggcgtccatc
      361 gtggaggcgt tggaccgggt ttatgacgcg aaagacatcg cggccgaatt ggtgagcggg
      421 catcgaaaat gaaggctgaa gcaaacatcg aacaatcaac atggaacgct ggttcccgca
      481 gcttgatctg tcctcctttg gttttgcttg ccgcaagcgg cgcgcggacc tattatgatg
      541 gcgttgttgt gctggaaatt tttaacgttc cggcgtccgc gcgtgtagct caattggata
      601 gagtgacggc ctccgaagcc gcaggttgtg ggttcaactc ccgccacgcg caccattttc
      661 tgatgatcga aacatgaaat cgtttttgcc atggctgctg gtcgttgccc tgattgccgg
      721 cgtgtatttt ctctacgccc aagtcaagga cagggacgca caagtcgcaa agctcagcca
      781 ggacaatctg gaagtggcgc aactccgtgc cgaaaatgag gaactgaaaa aaattccgct
      841 tcaaacgcag gaactggacc ggctgcgcaa agagcacgac gaattgctcc ggttgcgcaa
      901 cgaaacccag cgcaccaagg agcaggtcaa acaactcacg gcacaactcg ccgcggccca
      961 ggcccaaact gctgccgtgc aggcccaaaa cgagaaggcg caacagatag aacagcaagc
     1021 cactcaactc gccaacgaaa accaggactt gcgcaagcaa gcagatcgtg ccacctcgga
     1081 ggaaaaagtg gcccagcagc aaattgcaac ctgtgccaac aacctgcgca taattgacgc
     1141 tgccaagcag caatgggcct tggaaaacaa gaaaaccgcc acggacatcc ccacgccaga
     1201 agacatcgcg ccgtaccttc caaaccctgc gcagattgtt tgccctgcgg gtggcgttta
     1261 tagcatcaac gcaatcaaca ccgcgccgac ttgcagcgtg ccgtcgcata atctcccaaa
     1321 atgacggatt atcatgcggc ggtaacaaac accggtatgt tgtcaatgca ttttggttga
     1381 aatcgatgga acgggaacgg aagcggaaat tttcccgctt tccttttgcg aaccatggcc
     1441 taatgtttct gaacatataa cgcatgaata cgagcataga ggaagccttg tcggggatcg
     1501 cgaaccagtt gcgcattgat tccataatgg ccacaacggc ggcagggagc ggtcatccga
     1561 gctcctgctg ttcggcggct gaccttgtgg cagcgctgtt tttcggccac atgcggttcg
     1621 atccgaaaaa tcccaaatat cccaacaacg atcgtttcat tttgtcgaag gggcacgccg
     1681 cgccgttgtt gtatgcggct tgggccgaac tggaatttat tccaagagaa catctgctga
     1741 cgttgcgccg gttggacagt gatctggaag ggcatcccac gccgcggctg ccattcgtgg
     1801 acgtggcgac cggctcgttg ggccaaggct tgggcgtcgg cgcgggcatg gcgttgtgcg
     1861 cgcggctgga caagctggat tatcgcacat acgtgctgat gggcgatggc gaatgcgccg
     1921 aaggctcggt ctgggaagca gcgtccctgg caggtattta tcagttgaac aacctggtcg
     1981 cgattgtgga tgtcaaccgt ctcgggcaaa gcgagccgac agccttgggc tacgatgtgg
     2041 atgcttaccg aaagcgtttc gaagctttca actggcggac cgaggtcatc aacggccatg
     2101 acatggagga aatcttggaa gtgcttggcg ccgccggcct cggcaaacaa ccgctcgcca
     2161 tcatcgcaaa aacgcagaag ggccacggcg tttccttcat ggaagacaag gaaggctggc
     2221 atggcaaggc gctttccaaa gaagaagccg aaacggcgat cgccgaattg caacccaagg
     2281 ccaggaccgg attgccggaa ccgattcccg ctccttcgga attgccgctg ccaaagaatg
     2341 aagcgccttc gagctatccc gcgctgaatt acaagctcgg cgattcgatc gcgacgcgcg
     2401 aggcttacgg caatgcgctc ttgcgcatcg gcagcgttga cgaacgcatc gtggctgtgg
     2461 atggcgacac gaaaaattcc accttcgcgg acaagttttt caaaaagttc cccgaacgct
     2521 ccactgaatg ttatatcgcg gaacaaaaca tggtcggcgt ggcaacgggc tacgcgacac
     2581 gcgggaaggt gccgtttgcc tcgacgttcg cctgtttttt cagccgcgct tacgaccaga
     2641 tccgcatggc gggaatctcg atggcgaatg tgaaactggt cggctcgcac gtcggcgtga
     2701 gcatcggcga ggacggcacg tcgcaaatgg ctttggaaga tatcgccatg atgcgcgcgg
     2761 tggcgggaag tgtggttctt tacccgagcg atgccgtgag cgctgaaaaa ttggtggaac
     2821 aaatggcgtt gcacaagggc gtttcgttcc tgcgcacgtc gcgaccaaag acgccggtga
     2881 tctacggcaa tgacgaatcg tttcccatcg gcggcgcaaa aattcgcaag caaggcgccg
     2941 gagacaaagt gaccgttgtg ggcgcgggcg tgacattgtt tgaagctttg aaagccgccg
     3001 atgagctggc caaggatgga attggaatca cagtgattga cgcctacagc gtcaagccgc
     3061 tgggcaaaga tgttatcaaa gacgccgcgc aaaagacggg caatctcgtg gtcaccgtgg
     3121 aagaccatta cgcggaaggc ggtttgggcg atgctgtcgc cggcgagttg agcgaggatg
     3181 gcatcaaagt gcacaaactg gccgtgcgcg aactgccgca ttccggcaag cccgaagaat
     3241 tgctcgccaa gtatggcatt gattcggcag caatcgtgaa agcggtcaag tctctggttt
     3301 aacgggtcat cgagacctct gcgcggcttc ttcgcctcca tatttggcat gtttccagct
     3361 tcttgcatgg catggaaatg tccggtcatg ccactccaaa actgcggctt ctcgccgtct
     3421 ttctgatctt ttttgtcagt gccggggcac tgctggccca gccgttggtt tgcgccatct
     3481 gcggcaagcc gatttacgga aattattacg ttaatgaaga ccaaattgag catgtgcaga
     3541 agaatgtttg ctcgaattgc aatgccgtcg aagcgcggtg ttattattgc cagttgccgg
//
LOCUS       DASZMM010000176         5709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2825485, whole genome shotgun sequence.
ACCESSION   DASZMM010000176 DASZMM010000000
VERSION     DASZMM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825485"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="trpB"
                     /locus_tag="VG754_04255"
     CDS             <1..990
                     /gene="trpB"
                     /locus_tag="VG754_04255"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWF18450.1"
                     /translation="LGGAKIYLKREDLLHTGAHKINNAMGQILLAKRMGKTRIIAETG
                     AGQHGVATATVSAMFGLKCVIYMGEVDCQRQMLNVYRMKMLGAEVVPVKAGQRTLKEA
                     VNEAMRDWVTNVRSTHYILGTAYGAHPYPIMVRNFQRIIGDEARRQILEQEERLPDLL
                     IACVGGGSNAIGLFYPFLEDANVKMVGVEAGGLGIQPEKHAARFQGGSLGVLQGTRSY
                     ILQDENGQIQLTHSVSAGLDYAAVGPEHAWLRDQNRVEYTYATDEQALGAFQKLARLE
                     GIIPALESSHAIAEVIQCAPKMGKDKLIIVNLSGRGDKDVAQAADKIGLKMPE"
     gene            complement(1010..2323)
                     /gene="xylA"
                     /locus_tag="VG754_04260"
     CDS             complement(1010..2323)
                     /gene="xylA"
                     /locus_tag="VG754_04260"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HWF18451.1"
                     /translation="MTSAFPDISKISYEGPRSKNPLAFKHYNAREIVEGRTMQEWLRF
                     SVVYWHTFRNRLSDPFGLGTAQRPWDDGTESVKNAQNRARVAFEFIEKLGAPFYAFHD
                     RDVAPEGASLKESNKNLDAVVKVLKAEQERTGIKLLWGTACLFAHPRYAHGAATSCNA
                     DVFAYGAAQVKKALEVTHELGGEGYVFWGGREGYSTLWNTDMKRELDHLAQFLHMAVE
                     HKKKIGFKGQFYIEPKPKEPTKHQYDSDATACLNFLREYDLIDHLKLNIETNHATLAG
                     HTMQHELEAAAAAGALGSIDANTGDPLLGWDTDQFPTDIYLTTQCMLSIMKMGGLTTG
                     GVNFDAKVRRESFEPVDLFHAHIGGMDAFARGLKIAAAIRKDGRLAEFVRQRYSSWDS
                     GIGAKIEKGKTNFQELEAYMLKKGETSPNSSGRQEFLENLINEFI"
     gene            2541..3140
                     /locus_tag="VG754_04265"
     CDS             2541..3140
                     /locus_tag="VG754_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWF18452.1"
                     /translation="MIPLLLVLGVLFFIALMAIFWAIGGYNNLVSLRNRFKNAYAQID
                     VQLKRRYDLIPNLVETAKGYLKHERETLEGVIAARNVAANANVKAAQNPGDASAMKDL
                     SGAETALSGSLGRLFAVAEAYPDLKANQEMMQLMEELTSTENKVAFARQAYNDSVMVY
                     NTARETFPTNIIANTFNFAAAEMFVVDKPEQKEAPKVSF"
     gene            3145..5055
                     /locus_tag="VG754_04270"
     CDS             3145..5055
                     /locus_tag="VG754_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWF18453.1"
                     /translation="MPTPDFFTRQELARRHTKLLEVYFAFAIIGTILLTYAAVAFLFA
                     LKGGNPADPELLFGVVMATLAIIIGGSLYKTAQLSSGGKAVAEMLGGQPINANTSDPD
                     ERKFLNVVEEMSIASGTPVPQLYVLPDEPGINAFAAGHTTGDMVICATRGSLKFLKRD
                     ELQGVIGHEFSHILNGDMKLNLRLMGLVFGLLCLAIFGRILLNTRGRKNPLPLLGLAL
                     LAVGSLGVFFGKLIKSAVSRQREFLADASAVQFTRNPLGLANALKKVGGAGSRIEDPN
                     AEDASHLFFANGLNESILNMFSTHPPLDERIHALDPAWDGKFIAVDFSALRSEETPAT
                     TRNAPPTIPSAITRTILPGQLTGIIGAATAAAALGNAALRAPHASLSYAADWRSKLPP
                     VLEAAAREPMSAVALIYALLLSRDNTMRATQLQQLQKQSAPGICDETVKLFANVADLE
                     DRARLPLASLTMTALRRLSHLQFAEFERNIQYIIQSDQQLELFEYALQKIVLRQLEPN
                     FLPPKHTVVQYYVLKPLVPDCVALLSALAYAGQHEPAQIEAAFRQGTIKLNMPELALS
                     APTEYNLQIIDAALNHLNQASPQLKNLVLNACAETVAADGVIEESEAELLRAIADTLD
                     CPIPPFLHLEGA"
     gene            5149..>5709
                     /locus_tag="VG754_04275"
     CDS             5149..>5709
                     /locus_tag="VG754_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF18454.1"
                     /translation="MTQNIVTIRNLSKIYRQGDINVTALHNVSLDIKAGEFLTLMGPS
                     GSGKSTLLHIIAGIDRPTSGECRVQDVDVTRLNESQLADWRNQNVGFVFQTFNLIPVL
                     TAYENVELPLLLTGLSRRQRREHVQTALELVGLSERARHLPRQLSGGQEQRVAIARAL
                     VTSPALIVADEPTGNLDSQSAHDALGL"
BASE COUNT         1206 a         1723 c         1539 g         1241 t
ORIGIN      
        1 ctcggcggcg caaaaattta tttgaagcgc gaagaccttc ttcacaccgg cgcgcacaag
       61 atcaacaacg cgatgggcca aatcctcctg gccaaacgca tgggcaagac ccgcatcatt
      121 gcggaaaccg gcgcgggcca gcacggtgtc gcaaccgcca ccgtcagcgc gatgttcggt
      181 ttgaaatgcg tcatctacat gggcgaagtc gattgccagc ggcagatgct caacgtctat
      241 cgcatgaaaa tgcttggcgc tgaagtcgtg ccggtgaaag ccggccagcg caccttgaag
      301 gaagccgtca atgaagccat gcgcgactgg gtcacgaacg tccgctcgac gcattacatt
      361 ctcggcacgg cttacggcgc gcatccctac ccaatcatgg tgcgcaattt tcagcgcatc
      421 atcggcgacg aagcgcgccg gcaaatcctc gaacaggaag agcggttgcc ggaccttttg
      481 atcgcgtgcg tcggcggcgg ctcgaatgcc atcgggttgt tctatccttt cctggaagac
      541 gcgaacgtca aaatggtcgg cgtggaagcg ggcggtttgg gcatccaacc cgagaaacac
      601 gccgcgcgtt ttcaaggcgg ttcgctcggc gttttgcagg gcacgcgctc atacattttg
      661 caggacgaaa acggccagat ccagcttaca cacagcgttt ccgccggctt ggactacgcc
      721 gctgtggggc cggaacacgc ctggttgcgc gaccaaaatc gcgttgaata cacttacgcc
      781 accgacgaac aggcgcttgg cgcgttccaa aaactcgcgc gcttggaagg catcattccc
      841 gcgcttgaat cttcgcacgc catcgccgaa gtcatccaat gcgcgccgaa aatgggcaag
      901 gacaaattga tcatcgtgaa tctgtccggc cgcggtgaca aagacgtcgc ccaagccgcg
      961 gacaagatcg gattgaagat gcccgaataa cgaaagaggt ttgtttcctc tagatgaatt
     1021 cgttgatcaa attttccaaa aattcctgac gaccgctgct gttcggcgat gtttcgcctt
     1081 tcttgagcat ataggcttcg agttcctgga aattcgtttt gcccttttcg attttcgcgc
     1141 cgatgcctga atcccatgag ctgtagcgtt ggcgcacgaa ttccgccaac cgcccatcct
     1201 tgcgaatcgc cgccgcaatt ttcagtccgc gcgcgaacgc atccatgccg ccgatgtgcg
     1261 cgtggaacag atcaaccggc tcaaaacttt cgcgccgaac ttttgcgtcg aaattcacgc
     1321 cgcccgtcgt cagtccgccc attttcatga tgctcaacat gcattgcgtg gtgaggtaaa
     1381 tgtcggtcgg gaactggtcg gtgtcccagc cgagcagcgg gtcgccggtg ttggcgtcaa
     1441 tcgagccgag cgcgcccgcg gccgcggcgg cttcaagttc atgctgcatc gtgtgtccgg
     1501 cgagcgtggc gtggttggtt tcgatgttca acttgaggtg gtcgatcaaa tcgtattcgc
     1561 gcaaaaagtt caggcacgcg gtggcatctg aatcgtattg atgcttcgtc ggctctttcg
     1621 gtttcggctc gatatagaat tggcccttga acccgatttt ctttttgtgc tccaccgcca
     1681 tgtgcagaaa ttgggcaaga tggtccaact cccgtttcat gtcggtgttc cacaaggtcg
     1741 aataaccttc gcgtccaccc caaaaaacat agccttcacc gcccaattcg tgtgtcactt
     1801 ccagcgcctt cttcacttgc gcggcgccgt aagcgaacac gtccgcgttg caactcgtcg
     1861 cagcgccgtg cgcgtaacgc ggatgcgcga agagacaagc cgtgccccaa agcagcttga
     1921 tgccggtgcg ttcctgttcg gccttgagca ccttgacgac ggcatccaag tttttgttgg
     1981 attccttgag cgatgcgcct tcgggggcga cgtcgcgatc gtgaaatgca taaaatggcg
     2041 cgcccaattt ctcgatgaac tcgaatgcca cgcgcgcgcg gttctgtgcg tttttgaccg
     2101 attccgtgcc atcgtcccaa ggacgttgcg ccgtgccgag cccgaacgga tcgctcaacc
     2161 gattgcgaaa cgtatgccag tacaccacgg agaagcggag ccattcctgc atcgtgcggc
     2221 cctcgacgat ttcacgggca ttgtaatgtt tgaaggcgag cggatttttg gagcgcgggc
     2281 cttcgtagga aatcttggaa atatcgggaa atgcggaagt catagtttga aagggttggt
     2341 ctttgcgtta atttaaattt ggcatcaaag caacaagtat caggtttcgt catagcaaaa
     2401 aacaatcggc gcgcaagagc aagccgattg tggaataaac gacctgcgtt ttgctttaca
     2461 atttcgcctt caaaatataa ttgcctctgg ccttggtttg accaactctg tggtgtgact
     2521 ttgaaaacca aaggaccatt atgataccgt tactgctggt gttgggagtg ctgttcttca
     2581 tcgcactgat ggccattttt tgggccatcg gcggttacaa caacctcgtc tcgctgcgca
     2641 accgcttcaa gaacgcttat gcgcagattg atgtccagct caagcgccgt tacgacctga
     2701 tcccaaatct cgtcgagacc gccaaaggtt atctgaagca cgagcgcgaa acgctcgaag
     2761 gcgtgattgc cgcccgcaac gtcgccgcga atgccaacgt caaggccgcg caaaatcccg
     2821 gcgatgcttc cgccatgaaa gacctttcgg gcgcggagac ggcgttgagc ggaagtcttg
     2881 gccgcctttt tgccgttgcc gaggcgtatc ccgacctcaa ggccaaccag gagatgatgc
     2941 aactcatgga ggaattgacc tcgacggaaa acaaggtcgc gttcgcccgc caggcttaca
     3001 acgattcggt gatggtctat aacaccgcgc gcgaaacatt cccgacaaac atcatcgcca
     3061 ataccttcaa cttcgccgcc gccgaaatgt tcgtggtgga caagccggag caaaaggagg
     3121 cgccgaaggt atcgttctga tgccatgcca acgccggatt ttttcacgcg ccaggaactg
     3181 gcacgccgcc acacgaagct gctcgaggtt tacttcgcgt tcgcgatcat cggcactatt
     3241 ctgctgacct acgcggcggt cgcctttttg tttgcgctca agggcggcaa cccggcggac
     3301 cctgaattgt tgtttggcgt ggtgatggcg acgcttgcga tcatcatcgg cggaagcctt
     3361 tacaagaccg cgcagctttc cagcggcgga aaagctgtcg ccgaaatgct cggcggccag
     3421 cccatcaacg cgaacacttc cgaccccgac gaacgcaaat tcctgaatgt cgttgaggag
     3481 atgtccatcg cttccggcac gcccgtcccg caattatacg tgctgccgga cgagcccggc
     3541 atcaatgcct tcgccgccgg ccataccacg ggcgacatgg tcatctgcgc gacgcgcggc
     3601 tcgttgaagt ttctcaagcg cgatgaactg cagggcgtca tcggccacga gttcagtcac
     3661 atcctcaatg gcgacatgaa attgaacctg cgcctgatgg gtttggtttt cggcctgctc
     3721 tgcctcgcga tttttggccg catcctgctc aatacacgcg gacgaaaaaa tccgttgccg
     3781 ctccttggtc ttgccttgct tgcggtcggc tcgcttggag tttttttcgg caagttgatc
     3841 aagagcgccg tgagccgcca gcgcgaattt ttggccgacg cttccgccgt gcaattcacg
     3901 cgcaacccgc tcggccttgc caacgccttg aaaaaagtcg gtggcgcagg ctcgcgcatt
     3961 gaagatccca acgccgagga cgccagccat ctttttttcg ccaatggcct gaatgaatcc
     4021 atcctcaata tgttttccac gcacccgccg ttggatgaac gcatccacgc gctcgatccc
     4081 gcgtgggacg gcaagttcat tgccgtggat ttctcggcgt tgcgctccga agaaactccg
     4141 gccaccaccc gaaatgcgcc gccgacaatc ccgtccgcga tcacccgcac aatcctcccc
     4201 gggcaactga ccggtatcat cggcgcagcc acggcagcgg cggcacttgg caatgccgcg
     4261 cttcgcgctc cacatgcctc gttgagttat gcggccgatt ggcgttccaa actgccgccc
     4321 gtgcttgagg ccgccgcgcg cgagccgatg agcgccgttg cgttgattta tgcgcttctc
     4381 ctgagccgcg acaacaccat gcgcgcgacg caactccagc aattgcaaaa acagagtgcg
     4441 ccgggcattt gtgatgaaac ggtaaaattg ttcgccaacg ttgccgacct tgaagaccgc
     4501 gcccgcttgc cgcttgcgag ccttacgatg accgcgttgc gccgcctctc gcacctgcaa
     4561 ttcgccgagt tcgaacgcaa catccaatac atcatccaaa gcgatcagca gctcgaactc
     4621 ttcgagtatg cgctgcaaaa aatcgtgttg cgccagcttg aaccgaattt cctgccgccc
     4681 aaacataccg tcgttcaata ctacgtgctg aagccgctcg tgccggattg cgtcgcgctg
     4741 ctttccgcgc tggcttatgc cggccagcac gagcctgcgc agatcgaagc ggcgttccgc
     4801 cagggcacga tcaaattgaa catgcccgaa ctcgccctct cagcgcccac cgaatataac
     4861 cttcaaataa ttgacgccgc gctcaaccac ctgaaccagg cctcgcctca acttaaaaac
     4921 cttgtcttga acgcctgcgc cgaaactgtc gccgccgatg gagtcatcga agaaagcgaa
     4981 gccgaacttc tccgcgccat cgccgacacg ctcgactgtc ccattccgcc attcttgcat
     5041 cttgagggtg cttgaacgag tttgaaactt gcatttacca tgcactcgcg agcgtgctgg
     5101 tttccgattc gtaacgatgt aacattgcaa ccatttaacc gtcctttcat gacccaaaac
     5161 atcgtcacca tccgcaatct ctcgaaaatc taccggcagg gcgacatcaa cgtcaccgcg
     5221 ttgcacaacg tttcactcga catcaaggcc ggcgaatttt tgacgttgat gggaccgtcc
     5281 ggctccggga aatccacgct gctgcacatc attgcgggca ttgatcgccc gaccagcggg
     5341 gaatgccgcg tccaagatgt ggatgtgacg cggttgaatg aatcccagct tgccgattgg
     5401 cgcaaccaga acgtcggctt tgttttccag acattcaatc tgattccggt gctgaccgcg
     5461 tatgaaaatg tcgaactgcc gttgctgttg accggcctga gccgccgaca acgtcgcgag
     5521 catgtgcaga ccgcgttgga actggttgga ttgagcgaac gcgcgcgtca tttgccgcgc
     5581 cagctttccg gtggacaaga acagcgggtt gccatcgccc gcgcgctggt cacttcgccc
     5641 gcgctgattg ttgccgacga accgaccggc aacctggatt ctcaatccgc ccatgatgcc
     5701 ctcggcctg
//
LOCUS       DASZMM010000177         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1174934, whole genome shotgun sequence.
ACCESSION   DASZMM010000177 DASZMM010000000
VERSION     DASZMM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174934"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(493..789)
                     /locus_tag="VG754_04280"
     CDS             complement(493..789)
                     /locus_tag="VG754_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18455.1"
                     /translation="MTRHCHKCGWEWTLAGQPGRSESCHRCGADLRVCLNCVSYDARA
                     AHQCRDRRADPVEEKHVGNFCEYFDFARRNWVAKQENNSREDAARAQLRKLFGD"
     gene            1140..2675
                     /locus_tag="VG754_04285"
     CDS             1140..2675
                     /locus_tag="VG754_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF18456.1"
                     /translation="MSDCAREAVAEKVGMERQKLDDPFRVLFEANPLPMWIFDSNTLV
                     FLAVNDAAIQHYGYSREQFLRMTLRDVRPPEDIDSLQHALEKVSRQQGLIDVGEWRHL
                     RRDGSMIYVEICSQPVLYAGRNARLAMLNDITERKMTEGALRNSEARLKEAQHVAGLG
                     SWECYGSHGGTIYSDEMYRIFGFPRGTKMDTDAIVSTIHPEDRARMRGLLEGIKKDPH
                     NFDTMFRVVRPNGQVRQIHAQGELTYDSAGKPTKMTGTAVDVTDRLEAEKERLQNARL
                     QAANRELEAFSYSVSHDLRSPLRSIDAFGKLLTEEYGAQLDDNARSYLDLILSSTQRM
                     NQLIDDLIEFSRVTSTQIDRTEVDLSALAQTIARDLQRTMPSRKVEWLIAPDLKAHAD
                     RRLVRIALENLVGNSWKFTGKTEAARIEFGATRDGNETVYFVRDNGAGFDMEFASRLF
                     GVFQRLHSETDFTGTGIGLAIVQRIIARHGGRIWAEGVVNQGSTFYFTLPGKHPQLCK
                     AKV"
BASE COUNT          645 a          741 c          779 g          626 t
ORIGIN      
        1 ccgcgttcgg cggagctggt gctcttgtca atggcggccc tggtacagtc ggcacactct
       61 tcaattctta cgaacttctc gcgaacattg tttacaagtt ctagttagtt cagttctgct
      121 caacccctgc cggtcctccc ggcaggggct tttttttgga aaaaaaggga taacatcgaa
      181 cgctcctttt ccttggcaat caacatcctg tcgaatccgt cgttctgatt ggttctgttg
      241 catcgccgcc cagcgccgtc aggcgcgaca tctatgtaga aaccaaagaa gataaaaaat
      301 ccgagctccg tcaggacggc atatcccgca aattattcct cgtcttgaat aaatggatcg
      361 caggtcggat gccacatcga attttttcca ccgcgacgta atcctgttgc ccaggcgcag
      421 aagatgccgc tcctgacgga gcttgaaaat ccaccttgtt ctacaaagat gtcacaccta
      481 cggtgttcgt cttcaatccc cgaacaattt cctcaactgc gcgcgggcgg cgtcttcgcg
      541 ggaattgttt tcctgtttcg cgacccagtt gcggcgcgcg aaatcgaaat attcgcagaa
      601 atttcccaca tgcttctcct ccaccggatc ggcgcgccga tcgcggcatt gatgcgccgc
      661 gcgggcatcg tagctgacgc aattcaggca gacccgcaag tccgcgccgc agcgatggca
      721 actttcgctc cgtcccggtt gtcccgccag cgtccattcc cagccgcatt tgtggcaatg
      781 tcgagtcatg gtttcatgct atgatgaacg acgccgtttt tccagaccca aacgccacga
      841 agaggtttaa agtttaacca cgtaatacgg gtcaaggttc aaagttcaaa gttggcaggt
      901 caattttggc cttcgctcga atagaaaatg aggctcgccc tcacggcacg gtgcaaagat
      961 atgggtaaca gattgatgca tggacatagg caacactttt ttgatggatt ttggttgttt
     1021 ctgcggagat gccgtggaaa aaactttgtt tgatagggta ttcaaagcgg aaatcaccca
     1081 ccataagaag tccccatgac aagagtttcg caatcagccg ttctactggt atattcagtt
     1141 tgagtgattg tgcccgcgaa gcggtcgcgg agaaagtcgg tatggaacgg caaaaacttg
     1201 atgatccatt ccgggtattg ttcgaggcaa atccgttgcc gatgtggatt tttgattcca
     1261 acacgcttgt atttctggcg gtaaacgacg cggcaatcca acattacggt tattcacgcg
     1321 agcaattttt gcggatgacc ctccgggatg tccgtccgcc agaggacatt gactctctcc
     1381 aacacgcgct ggagaaagtc tcgcgccagc agggtttgat tgatgtcggc gaatggcggc
     1441 atcttcgacg cgacggctcg atgatttatg tggagatctg ctcgcaaccg gtcctgtacg
     1501 ccggcaggaa tgcgaggctc gcaatgctca acgacatcac cgaacgcaaa atgaccgaag
     1561 gcgcgttgcg caacagtgag gcgcggttga aggaagcgca gcacgtcgca gggttgggaa
     1621 gttgggaatg ttatggaagc catgggggca ccatctattc agacgaaatg tatcggatat
     1681 ttggctttcc gcgaggcacg aagatggaca ccgatgccat cgtcagcacg atacatcccg
     1741 aagaccgcgc tcgaatgcgc ggattgttgg aaggaattaa aaaagacccg cataattttg
     1801 acaccatgtt tcgcgtggtc aggcccaacg ggcaggtgcg gcaaatccat gcccaaggcg
     1861 agttgacgta tgacagcgcg ggcaaaccaa ccaaaatgac gggaacggcg gtggatgtca
     1921 ccgaccgatt ggaggcggaa aaggaacgtc tccagaacgc gcggttgcag gcggcgaaca
     1981 gggaactgga ggcgttttca tattcggtgt cgcacgattt gcgctcgccc ttgaggagca
     2041 tcgacgcgtt cggaaaattg cttacggaag aatacggcgc gcaactggac gacaacgcca
     2101 ggagttatct cgatttgatc ctgtcgagca cgcagcgcat gaaccagttg atcgacgacc
     2161 tcatcgaatt ctcgcgcgtg accagcacac agatcgaccg caccgaggtg gacttgagcg
     2221 cgctcgcgca gaccatcgcc cgggatttgc agcggacaat gccaagccgg aaggtcgaat
     2281 ggctcatcgc gccggacttg aaagcgcacg ccgaccgtcg gttggtccgg atcgcgttgg
     2341 aaaacctggt tggcaactcc tggaaattca ccggcaagac cgaagccgcg cgcatcgagt
     2401 tcggcgcgac gcgggacggg aatgaaacgg tttattttgt gcgcgacaac ggcgcgggtt
     2461 tcgacatgga atttgcgtcg cgattgttcg gcgtgttcca acggcttcac tcggaaaccg
     2521 actttacagg aacgggcatc ggattggcga ttgtccagcg catcatcgcc cggcatggcg
     2581 ggcgcatctg ggcggaaggc gtggtgaacc aaggctcgac tttttatttc acgctgccgg
     2641 ggaaacaccc ccaactttgc aaggccaagg tttaaaatgg gtgcatcttg ccactgttct
     2701 tgagaatgta caggcatggt ttggatgacg cccgccctcg ggcgcatctc agtttcttgc
     2761 acaccggagt ggttgaacat ttccttcccc c
//
LOCUS       DASZMM010000178         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1176351, whole genome shotgun sequence.
ACCESSION   DASZMM010000178 DASZMM010000000
VERSION     DASZMM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176351"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VG754_04290"
     CDS             <1..436
                     /locus_tag="VG754_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWF18457.1"
                     /translation="RWRDGKIVRDNNGGGGTHGSFFSWPVETREPEHPIMKGLPEKWM
                     HTPDELYATLVGPAENVTVLATAKSDVTQQNEPILMVINYGKGRIFHTTMGHNELSMK
                     DVGFVTTLNRGAEWAATGNVTQKVPDNFPTADHTSVWNPPGK"
     gene            complement(441..842)
                     /locus_tag="VG754_04295"
     CDS             complement(441..842)
                     /locus_tag="VG754_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWF18458.1"
                     /translation="MPAQRESIEKRALWRCEYCHAPQHVTGYNFHLEHIVPRGKGGSD
                     HHSNRALACANCNFSKSNHLSAPDPKNGRLERLFNPRSDKWQEHFAFSRATLKISGKT
                     SIGRATVTLLRMNSERQLEARALWVELQVYL"
     gene            complement(851..1321)
                     /locus_tag="VG754_04300"
     CDS             complement(851..1321)
                     /locus_tag="VG754_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18459.1"
                     /translation="MSGINAYSHLTTVLNVRKIAIPELRRQMLKLGVPVNIKSLYRLA
                     SPEPLQKIDLRILGAICRTCHVGIQEVIDFNKPSMALQRLGSSDQKRLDELMSGNNDG
                     MLDSAERKELEELAGKAHQITIANARLLARQRRTNRSHAAGLRNRHRRKLRHAA"
     gene            complement(1363..2259)
                     /locus_tag="VG754_04305"
     CDS             complement(1363..2259)
                     /locus_tag="VG754_04305"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HWF18460.1"
                     /translation="MKPPRRKPQTLAELRALQRLMAQAVMRPLTASQRMRPEWSDGSA
                     ADKIAGSFIKPNDRLTSFERLEIYNRQYWFRVMECFAEDFCGLRAVLGEERFHDLAVA
                     YLMNHPSRSFTLRELGSRLVEFIQAEPRWTAPQTELALDMVRLEWAHIEAFDNAAKPP
                     ITAADLEGRAAAQIHLQLQPHITLLQLAYPLDDYLIKLRENTRLRGEASNAIADEHSH
                     SNARTPRLPERRVIRVAMHRFENIVYYKRLQARQYTLLLAIQKGATLEKALQTLPMSG
                     PTPPIGEWFKNWSALGWFWQEL"
     gene            complement(2240..>3037)
                     /locus_tag="VG754_04310"
     CDS             complement(2240..>3037)
                     /locus_tag="VG754_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HWF18461.1"
                     /translation="LRIPHYRHILKEKPVVDWFEIISENFMVEGGRPLEILDSILEQY
                     RVVQHGVSMYFGSAEPLNREHLRKLKRLVRRTKTPWLSDHLCWGSVDGRYTHDLLPMP
                     YTFAAAKVAAQKIREVRDFLEVPIAVENVSSYAEFHVSEMTEWEFLGEVVERADCGIL
                     LDVNNIYVSSFNHGFNPYDYLDNIPMERVAQMHIAGHSRYRKYILDTHDHAVIEPVWK
                     LYARAARLAGRTATLLEWDAHIPSFDEVHHEALKANQFVSQLETAAA"
BASE COUNT          672 a          859 c          835 g          671 t
ORIGIN      
        1 tcgctggcgc gatggcaaaa ttgtgcggga caacaacggt ggcggcggca cgcatggctc
       61 atttttttca tggccggtgg aaacccgcga gccggagcat cccatcatga aaggtttgcc
      121 ggaaaaatgg atgcacacgc ctgacgaact ttacgcgacg ctggtcggtc ccgccgaaaa
      181 tgtcaccgtg ctcgccacgg ccaagtccga tgtcacacaa caaaacgagc ccatcctgat
      241 ggtcatcaat tacggcaaag gccgcatctt ccacaccacc atgggccaca atgaactttc
      301 catgaaagac gtcgggttcg tgaccacatt gaatcgcggc gctgaatggg ccgccaccgg
      361 caacgtgacc caaaaagttc ccgacaattt tcccaccgcc gaccacacca gcgtttggaa
      421 cccgcccggt aaataaacac ttacaaataa acctgcaatt caacccacaa agcgcgggct
      481 tccaattgcc gctcgctgtt catccggagc agtgtgaccg ttgccctccc aatcgaagtt
      541 ttgccgctga ttttcagagt ggcccgggaa aaggcaaaat gctcctgcca tttgtcggat
      601 cgagggttga aaagccgttc cagccgtcca ttttttggat caggcgcgga cagatggttt
      661 gatttggaga aattgcaatt ggcgcatgcc aaggcgcgat tggagtgatg gtcgctcccg
      721 ccttttccac gtggcacaat atgctccaga tgaaagttgt agcccgtgac gtgttgcggc
      781 gcatgacagt attcgcatct ccagagcgcc cttttttcga tggattcccg ttgtgcaggc
      841 atcgaaagga tcaggcagcg tggcgaagct tccggcgatg acgattacgc agcccggctg
      901 catgggagcg gttcgtccgg cgctggcgcg caaggagccg tgcgttggcg atggtgattt
      961 gatgggcttt cccggcaagc tcctccagtt ccttgcgttc agcggaatcc agcatcccgt
     1021 cgttgttccc ggacatcaat tcatccaaac gcttttgatc ggaagatccc agccgttgca
     1081 gcgccatgct gggtttgttg aaatctatca cttcctggat gcccacatgg catgtccggc
     1141 aaatggcccc caaaatgcgc aaatcaattt tctgcagcgg ttccggcgag gcaagccggt
     1201 aaagactttt aatgttgacc ggcacgccca gcttcaacat ttgccgccgc aactcaggta
     1261 tcgcaatctt ccgcacgttg agcacggttg tcagatgcga ataagcatta attccactca
     1321 tctggtagtc ataccatctg gatggtaata tgcaaactat tttcaaagct cttgccaaaa
     1381 ccatccgagc gcggaccaat tcttgaacca ttcgccgatg ggtggcgtcg gaccgctcat
     1441 cggcaatgtt tgcaaggcct tttcgagcgt cgcgcccttt tgaatcgcga gtaacagagt
     1501 gtattgccgc gcctgcaaac gtttgtaata gacgatgttt tcaaaacgat gcatcgcaac
     1561 ccggatgacg cggcgttcgg gcaggcgcgg cgttcgcgca tttgaatggg aatgttcgtc
     1621 cgcaatggca ttgctcgctt cgccgcgcag gcgggtattt tcgcgcagtt tgatgaggta
     1681 atcgtcgagc ggataagcga gttgcagcaa agtgatgtga ggctggagtt gtaaatgaat
     1741 ttgcgcggcg gcgcggcctt ccaaatcggc agcggtgatg ggtggcttgg ctgcgttatc
     1801 gaacgcctcg atgtgcgccc attccaatcg caccatgtcg agcgccagct ccgtttgcgg
     1861 cgcggtccaa cgcggttcgg cttggatgaa ttcgacaagt cgcgaaccaa gctcgcgcaa
     1921 cgtaaaactg cgcgacgggt ggttcatcaa gtaagccacc gccagatcgt gaaaccgctc
     1981 ttctcccaac accgcgcgca atccgcaaaa atcctccgca aaacattcca tcacacgaaa
     2041 ccaatattgc cggttgtaaa tctccaaccg ctcgaatgaa gtcaggcgat catttggctt
     2101 gatgaaactt ccggcgattt tatcggctgc ggacccgtcg ctccattccg ggcgcatccg
     2161 ttgggaagcc gtgagcggac gcatcaccgc ctgcgccatc agccgttgca aggctcgcaa
     2221 ttccgcgagc gtttggggct tacgccgcgg cggtttcaag ctggctgacg aattgattgg
     2281 ctttgagcgc ctcatgatgg acttcatcga aggacgggat gtgcgcgtcc cattcgagca
     2341 acgtcgcggt gcgtcccgcg agccgcgcgg cgcgcgcgta taatttccac accggctcga
     2401 tcaccgcatg gtcgtgcgtg tccaaaatat atttgcgata acgcgaatgc ccggcaatgt
     2461 gcatctgtgc gacgcgttcc atcgggatgt tatcgagata gtcgtaagga ttgaacccgt
     2521 ggttgaacga cgacacgtaa atattgttca cgtccagcaa aatgccgcaa tcggcgcgct
     2581 cgacgacctc gccgagaaac tcccattcgg tcatctccga aacatgaaat tccgcgtaac
     2641 tgctcacgtt ctcgaccgcg atgggcactt caagaaaatc ccgcacctcg cggatttttt
     2701 gcgcggcgac cttggccgcg gcaaaggtat aaggcatcgg caacaaatcg tgggtgtaac
     2761 gtccgtccac gctgccccag cagagatggt cggacaacca cggcgttttg gtgcggcgga
     2821 cgaggcgttt gagtttgcgc aagtgttcgc ggttcaacgg ttcggccgag ccgaaataca
     2881 tcgaaacgcc atgctgcacc acgcgatatt gttcgagaat gctgtcgaga atttccagcg
     2941 gacgcccgcc ttcgaccatg aaattttccg agatgatctc gaaccagtcc accaccggtt
     3001 tttccttgag gatatgccgg taatgcggga tgcgcag
//
LOCUS       DASZMM010000179         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1983374, whole genome shotgun sequence.
ACCESSION   DASZMM010000179 DASZMM010000000
VERSION     DASZMM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983374"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VG754_04315"
     CDS             complement(<1..1499)
                     /locus_tag="VG754_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWF18462.1"
                     /translation="MSNAAWVAILAAVFGGAMALAVALQARRSVAHWTFAAGMLVLAV
                     ESIFTWLSLNAAGANEPPEVVVRWETWRLMAGSRLPGLWLCFSLAYARGNSHEFLARW
                     KPVLIIAFLAPLGLAIFFRDDLVQLVAIQGARVARLSIPGLALNLLLLLSVVIVLMNL
                     ERTFRASVGTMRWRIKFMVVGLAVLFGVRAYTCIQMLLFNSIDLSWHCIDSGALLVAC
                     PLILRTLLRTGHFDVNVYPSHSVIHNSIILLLAGVYLLAVGAFANMVKWMGGNTSFAF
                     KAFVLLLGLVLLTVMLVSDRVRLYTKRFVSRHFQRPMYDYRTVWRRFTECTASRMDQM
                     ELCRATAHLVAEVFQVLSVTLWVVNENKDALTFAASTSLSDTKGAELQPTNAETAGVL
                     GALQNHPDPVDIDACKENWAAALRKCHPDDFHKGGSRICVPMMVGGEVLGVMTLGDRV
                     AGVPFLQQDLDLLKCVGDQVAASLRNLHLSGKLLQVKEMEAFQTMSAFFV"
     gene            1825..2520
                     /locus_tag="VG754_04320"
     CDS             1825..2520
                     /locus_tag="VG754_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HWF18463.1"
                     /translation="MAIPQLLPTTAQNETFGGVTYHIEGELVPALHLELSGTGIYFEH
                     HILLWKDPAVEVALRPMKGALKRMLAGMPFLLTGARGPGRIAFSRDGAGHVFGIHLQP
                     GAGVDVREHQWLAATDNVEYTFSRVRGAANILLGGTGFFIDTFSCPSREGILWLHGYG
                     NVFEVTLAPGEAIDVEPGGWIYKERSVKMDTMFQRLSTGLFASAGQLCWNRFTGPGKL
                     AIQSMYYHMEGGE"
     gene            complement(2588..>3174)
                     /locus_tag="VG754_04325"
     CDS             complement(2588..>3174)
                     /locus_tag="VG754_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF18464.1"
                     /translation="GDGSKYYIKLEQNNNGTNSYLVGITQLAYLDSVFSYGTPSNTWA
                     HLAFVASGTNVSLYANGAYQSSITTNNFPLPRGFIGTGSTGTRFLNVLHGALDDIQVY
                     NRMLNPSEINSIYSAGSAGLVRAPQVTQFTETNGTFTVNFEGLTSKTFTIYSSTNLTN
                     WSAVTTLSNPGGTNSYSEAIGTNNPEKFFRIGQP"
BASE COUNT          718 a          907 c          936 g          613 t
ORIGIN      
        1 acgaagaagg cggacatggt ctgaaaggct tccatttcct tgacctgaag caattttccg
       61 gagagatgga gattgcgcag gctcgcggca acttgatcgc cgacgcattt caacaaatcc
      121 aaatcctgct gtagaaacgg cacgccggcc acgcgatcgc ccagcgtcat cacgcccagc
      181 acttcgccgc ccaccatcat cgggacgcaa atccggctgc cgcccttgtg aaaatcgtcc
      241 ggatgacatt tgcgcagcgc ggcggcccaa ttttctttgc acgcatcgat gtccacgggg
      301 tcgggatgat tttgcagcgc gcccaacacc ccggctgttt cggcgttcgt cggctgcaat
      361 tccgcgcctt tcgtgtcgga gagcgaagtc gaggcggcga aggtcagcgc gtctttgttt
      421 tcgttcacga cccagagtgt gacggaaagc acttgaaaca cttcggcgac gagatgggcc
      481 gtcgcgcggc aaagctccat ctggtccatg cgcgaggcgg tgcattcggt gaagcgccgc
      541 cagaccgttc gataatcgta catcggccgt tgaaaatggc ggctgacgaa acgcttcgtg
      601 taaagccgca cgcggtcgga gaccaacatg acggtgagca acaccaaccc gagcaaaaga
      661 acgaaggcct tgaatgcgaa ggaggtgttt ccgcccatcc atttcaccat gttcgcaaat
      721 gcgcccacgg cgagcaaata aacccccgca agcagcagga tgatcgagtt gtggatgacc
      781 gagtgggagg gatagacgtt cacgtcgaaa tgccccgtcc gcagcagcgt gcgcaaaatc
      841 aatgggcacg ccacaagcag cgcgccggaa tcaatgcaat gccatgaaag atcgatgctg
      901 ttgaacagca gcatctggat gcatgtgtag gcccgcacgc cgaacagcac ggccagcccc
      961 acgaccatga acttgattcg ccaacgcatc gttcccaccg acgcgcggaa agttcgttcc
     1021 aggttcatca aaacgatcac cacgctgagg agcagcagca ggttcagcgc aagcccggga
     1081 atcgagagac gggcgacacg cgccccttgg attgcaacca attgaaccag atcgtcgcgg
     1141 aaaaatatcg caagacccag cggcgccaaa aatgcaatta tcagcaccgg cttccagcgc
     1201 gccaggaatt cgtgcgaatt gccgcgcgca tacgcaaggc tgaagcacaa ccacagtccc
     1261 ggcagacgcg agcccgccat caatctccac gtttcccagc gaacgacaac ttccggcggc
     1321 tcgttcgcgc cggcggcatt gaggctcaac cacgtgaata tgctctcgac ggccagcacg
     1381 agcattcccg ccgcgaacgt ccaatgcgcc accgaacgcc gcgcctgcaa ggccacagcc
     1441 agcgccattg cgccgccaaa cacagcagca aggatggcaa cccaggcggc gttgctcata
     1501 gatttttttt gggtgcgatc atttcatcat tccaaaggca ttggcctgga ttgtgcagac
     1561 aaacttcatg gaaatcacgc aaaaagcggg ccaatgtgga cgaatgaatt gggggggtgg
     1621 agtgttggag tgatgcaata acggttccgg acagcgctcc tttcattgac tttcccgtca
     1681 ggcattcgcc ggtgcaggcg agcctgacgg gaaagtaatg aaaccaaagg ggacatttta
     1741 aaagagtttt gacacgcgtt tggaaaaact tgacggctga cggccttggc attcaaatcc
     1801 ccgaaaacat caacccctaa aaccatggcc atcccacaat tactccccac gaccgcgcaa
     1861 aacgaaacat tcggcggtgt cacgtatcac atcgaaggcg aactggtgcc ggcgttgcat
     1921 ctcgaattga gcggcacggg catttatttc gagcatcaca tcctgctctg gaaagacccg
     1981 gcggttgagg tcgcgttgcg tccgatgaag ggcgcgctca agcggatgct cgcgggaatg
     2041 ccgtttctgc tcaccggcgc gcgcggtccg ggccgcatcg cattcagccg cgacggcgcg
     2101 gggcacgtgt tcggcatcca tctgcaaccg ggcgcgggag tggacgttcg cgagcatcaa
     2161 tggctcgcgg cgacggacaa tgtcgaatac acgttctcgc gcgtgcgcgg cgcggcgaac
     2221 atcctgctcg gcggcaccgg ctttttcatt gacacatttt catgcccgtc gcgcgaaggc
     2281 atcctttggc tgcatggtta tggcaacgtg ttcgaagtga cgcttgcgcc gggcgaggcg
     2341 attgatgtcg agccgggcgg ctggatctac aaggaacgct cggtgaagat ggacacgatg
     2401 tttcagcggc tctcgaccgg cctgttcgca agcgccggcc agctttgctg gaaccgcttc
     2461 accggcccgg gcaagctcgc gatccaatcc atgtattatc acatggaagg cggggaatga
     2521 atcaagcgag acaaggagcg tccgctttga tttcaaattt gaaacttggg atttcaaatc
     2581 cgcgccatca cggctgcccg atgcggaaaa acttctcggg attgttcgtg ccgatggctt
     2641 cgctgtagct gttcgttccg ccggggttgg agagcgtcgt caccgcggac cagttggtca
     2701 ggttggtcga ggagtagatg gtgaatgtct tgctggtcag gccttcgaaa ttcaccgtga
     2761 acgtgccgtt ggtttcggta aattgcgtca cttgcggggc gcgcaccaat cccgcgctgc
     2821 cggccgaata aatcgaattg atctccgacg ggttgagcat gcgattgtaa acctggatgt
     2881 catcgagcgc gccatggaga acatttagga accgggtgcc ggtagagccg gtgccgatga
     2941 aaccgcgcgg gagcgggaag ttgttcgtgg tgatggagct ctggtacgcg ccattcgcat
     3001 agagggagac gttcgtgccg ctggcaacaa atgccaggtg cgcccaagtg ttgctcgggg
     3061 taccatagct gaaaacggag tcgagatagg ccaactgggt aatgcccacc aagtacgagt
     3121 tggtgccatt gttgttctgc tccagcttga tgtaatactt gcttccgtcg ccca
//
LOCUS       DASZMM010000180         8070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_66478, whole genome shotgun sequence.
ACCESSION   DASZMM010000180 DASZMM010000000
VERSION     DASZMM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66478"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VG754_04330"
     CDS             <1..335
                     /locus_tag="VG754_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF18465.1"
                     /translation="YHEMCVQCHGAPGKEAGEIAKGLWPTAPNYNMITSDWTPAQLFW
                     ITKNGIKFTAMPGWGGTHSDEKIWDIVAFLQKLPNLSPTDYHQMETNTVGIQSSGDEQ
                     TNAPSAHH"
     gene            complement(470..3373)
                     /locus_tag="VG754_04335"
     CDS             complement(470..3373)
                     /locus_tag="VG754_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF18466.1"
                     /translation="MSAGMTVQAQYSNVVMGLHPAAYYPMNEVATPPAAAGSTITNYG
                     TDTSLNLNYAGDVIFGNASPLASQPADSSDYFNGGTSYATAAYDATLSSGPPFTIEGW
                     FLARNISSTECPTSDFEGNSPRSGWLIYMDISNPGQYTFRAYNQNGSTPSLSFNIGAP
                     GSIARDQWNYLAVVVDVSGGTTNVSGYLNGALVSGPTVLPSYVPSSGGTGGLSVGCRS
                     DISFGFDGNIDELAYYTNALSAATILSHYQAATNSSPSTPYDQLVLQQNPLAYFRFNE
                     ATPPLSSDYPAPLPVAHNYGTYGASANGFFTVGSNPGGAAGPTNIGFGGLPALELNTI
                     FASSGTAGPGIYCAPFNPELFNETNAMTVAFWAEVPANPNYFQTVIGRGDGSWRFDVD
                     QGGLPHWAAAPNSDLTGSTSLADTNWHFWAGVYDPVSGTGSLYIDGAVVVSAPWAALS
                     SSVQYPLLIGGAPDYDSAGRNFQGNIAQVAIFTNALTGAQINSLYAGAGNAPAAVSVL
                     ITSIAINENASTNITATAGGSLPISLQWYYTDTTQTTFPITGATNLTLNLDNVSFSQN
                     GYQYTVVASNAYGMATSAPVTLSVNTGSPVITSDLTPIFSGLPVGVSDQFSVVVTGSE
                     PFSYYWTLNSATIPGATNSSYAFNVAAGTNNYSVIVSNSYSAVTSSVAQVVGLTAAPP
                     VVTFGDSTMWQLNSSAGAGAAFPTLGGGSLLLTDGNGNEASTAFYVIPQYVGGFVATF
                     TYNETNGTADGATFMVQDSSAGTNALGGGGGALAYYGIGNSAAFEMNLYTGSSGGTGI
                     QFGTDGATPSSPIPQGPYTPTTPVSLTSGDPINVRLAYVGGVMHAKLTDATTGATYNT
                     THTFGDLTSILGNASGYVGFSGATGGLAAIQAVSNFQFTYTTPPVLSLTTSNNNALVR
                     WPVSVSSLFTLQQASVVTGPYTNIVTAPTVVNGTNQITVPVIGKSQFFRLILP"
     gene            complement(3764..4903)
                     /locus_tag="VG754_04340"
     CDS             complement(3764..4903)
                     /locus_tag="VG754_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWF18467.1"
                     /translation="MHNFEPLTKMDGLPKRTSLVRETANTLKDWIEAGILRGTLPGEL
                     PLKMRLGVGRDTLRLALRLLEREGWTTPTSRGRQRQVQIGHVPSTTRANSARLPVTFL
                     SPERIVDRQVLLEMEDLQMRLTKQGRDLRFASPNIFHLAHPDRLLERLVRQQPSAAWI
                     LYSTGEMVQRWFDERNIPAFIYGAPFTGINLPFTVNDWEEGTFHAGIQLLRHGHRIIG
                     IMENEHPSPGTVAAERGLRRALATIGTKGKLVILKEDRSPASVARAYELIFSLKQRPT
                     GLVLTNSAQLLTGYSWLASKGIRTPGDISLVAIANDSCFAEFHPPPCFYRCNSQVLAR
                     HIGHRVMELVDNGQVSQKSVRVHLEYVPGATIAPPPANLRQVPAE"
     gene            complement(4931..5635)
                     /locus_tag="VG754_04345"
     CDS             complement(4931..5635)
                     /locus_tag="VG754_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWF18468.1"
                     /translation="MKTMIRRMSAMMALCLIAVTKLQAQYVPVNPYQPYNPYQPYYTM
                     GPFYLGVDVGGVIVQDLKIRNTGSTVSFDPGVRGDFIFGCQVADQIAVEFETGTLWNR
                     MDKVDGVSFSSLGERADLYQIPFLANIVFRAPLNNGVSIYVGAGCGGVASELDLTQTD
                     NVGDRSHSYDTDATFAYQGKVGVSYAFAPNMEVDLSYKFLGTLSHTWFEGNSFLFANS
                     DPIYSHSILASFTWRF"
     gene            5836..6576
                     /locus_tag="VG754_04350"
     CDS             5836..6576
                     /locus_tag="VG754_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18469.1"
                     /translation="MKNVFDLIKGAMACLTLLVVLGMETKAVAQDYSGEATVVRLRGK
                     ARVSSDGKTWQTLKKGAHVPTGAMIQAAENSTLDLALGAAGGRSVSGEPAEGVLRLEP
                     DTLLNIDHLGAGTGAAQDVELDLRAGQIAGNIGRLPSGSRYEIKFSKGVAGVRSGIFH
                     INASGVVAVPAGETVVVILGADGNPITTIVNTGKEFDPSTGMMKDISKSSSATTAAPP
                     DRAEPPPAAGAPNPTPRGPGMGGSLRKF"
     gene            7288..>8070
                     /locus_tag="VG754_04355"
     CDS             7288..>8070
                     /locus_tag="VG754_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF18470.1"
                     /translation="MKLRLLLAAGFAAGWLAAFAPEQSSAENWAHWRGPLFNGSTTET
                     GLPVNWSTNENIAWVAPLPGLSGATPIVWGDRVFISSPDADKNLLLLCFNRKDGKLLW
                     QKTVAVGDRTKGRNNMASPSPVTDGKKVFVIFGTGDLAAYDFDGNELWKRNLGKEYGR
                     FANMWLYGSSPLLFEGKLYVQVLERNPRPDDYTSAIDAKPERESYLLCIEPKTGKNIW
                     RHVRPTDAKNESQEAYSSPIPDTGGKHAEIIIVGGNYTTAHDP"
BASE COUNT         1867 a         2148 c         2335 g         1720 t
ORIGIN      
        1 attatcatga aatgtgcgtt caatgccatg gcgctcccgg caaggaggcc ggggagatcg
       61 caaaaggcct ttggccaacg gctccgaatt acaacatgat cacttccgac tggactcccg
      121 cgcagttgtt ctggatcaca aaaaatggaa tcaaatttac cgccatgccc ggatggggag
      181 ggacgcattc cgacgaaaaa atctgggaca ttgttgcctt tctccaaaaa ctgccgaacc
      241 tttcaccgac cgactaccat cagatggaaa cgaacacggt gggaatccaa tcttccggcg
      301 acgaacaaac gaacgcgccg agtgcgcatc attaaacgtg aacactcacc gtcacttact
      361 ttgtgcgcgt cctacgggtt gatgaatatg tatgcaaaac aggccgggga ttttgtcccc
      421 ggcctgcgac gtttaaagga acgtttcgcc ttgaaccaaa gtcctttgat tatggcaata
      481 tcaagcggaa aaattgcgac ttgccgataa ccggcacggt gatttggttc gtaccgttca
      541 caacggtcgg agcggtgacg atgttggtgt aaggtcccgt cacgactgag gcctgttgca
      601 aggtgaacag cgaggagacc gagaccggcc aacggacgag agcgttattg ttgctggtgg
      661 tcagcgaaag gaccggcggg gttgtgtagg tgaactggaa gttcgagacg gcttgaattg
      721 cagccaaacc gccggtcgct cccgagaatc ccacgtagcc gctggcgttc cccaagatcg
      781 aggtaaggtc gccaaaggta tgggtggtgt tataggtggc gccggttgtt gcatcggtca
      841 atttggcatg catgacgccg cccacgtagg cgaggcgcac attgatcggg tcgccgctgg
      901 tgaggcttac cggggtggtc ggtgtgtagg gaccctgtgg gatgggactg ctgggcgtcg
      961 cgccgtccgt gccgaattgg atgcctgtgc cgccgctgga gccggtgtag agattcattt
     1021 caaacgccgc gctgttgccg atgccgtagt aggcaagagc tccaccgccc ccacccaagg
     1081 cgttggtgcc agcgctggaa tcttgcacca taaaggtggc gccgtccgcc gtgccgttgg
     1141 tttcattgta ggtgaaggtg gccacgaaac cgccgacgta ttgcggtata acatagaagg
     1201 cggtggacgc ttcattgccg ttgccgtccg taagcaacag gctgccgccg ccaagcgtcg
     1261 ggaaggctgc gccagcaccg gcggagctgt tgagctgcca cattgtgctg tcgccaaagg
     1321 tgacgacggg gggagcggcg gtcaagccaa ccacttgcgc gacagaactg gtgacggcac
     1381 tgtaggaatt gctgacgatg acgctgtaat tgttcgtgcc agccgccaca ttgaaggcgt
     1441 agcttgagtt cgtggcgccg gggatggtcg cggaattcag cgtccaataa taggagaacg
     1501 gctcgctgcc ggtcaccaca acggagaatt ggtccgaaac tcccacgggc aacccgctga
     1561 agattggggt taagtcgctg gtgattaccg gcgaacccgt gttgacggac agtgtcaccg
     1621 gcgcgctggt ggccatgccg tatgcattgc tggcgacgac ggtatattgg tagccatttt
     1681 gcgagaacga aacattgtca aggttcaacg tcaggttggt ggcgccggta atcgggaaag
     1741 ttgtctgggt cgtgtccgtg taataccatt gcagggagat gggcaggctg ccgccggccg
     1801 tggccgtgat gttcgtgctg gcgttttcat tgatggcaat cgaagtaatc agcaccgaaa
     1861 ccgcggcggg ggcgttgcct gcacccgcat aaaggctgtt gatctgcgcg ccggtcaaag
     1921 cgttcgtgaa aatggcgacc tgcgcgatat tgccctggaa gttgcgccca gcgctgtcat
     1981 aatcgggagc gccgccgatc aacaaaggat attgcacact gctggagagc gcggcccaag
     2041 gagcgctgac gacaacggca ccatcaatat acaaggaacc cgtgccggac accgggtcgt
     2101 aaactccggc ccaaaaatgc cagttggtat cggccagaga cgttgagccg gtcaaatcgc
     2161 tattcggagc ggcggcccaa tgggggaggc caccctgatc cacgtcaaaa cgccacgagc
     2221 catcaccgcg cccaatgacc gtttgaaaat aatttggatt ggcgggcact tcagcccaaa
     2281 aggcaactgt catcgcattg gtttcattga agagttcggg attgaacggc gcgcaataaa
     2341 tgccgggacc agccgtgccc gagctggcga aaatggtatt cagttccaga gcgggaagcc
     2401 cgccgaaacc aatgttcgtc ggaccggccg ctccaccggg attggagccg acagtgaaaa
     2461 acccgttggc gctcgcgcca taagttccgt aattatgggc caccggcaag ggagccggat
     2521 aatcgctgct caagggcgga gtggcttcgt tgaaccggaa ataagccagc ggattttgct
     2581 gcagcaccaa ttggtcgtaa ggagtagaag gcgacgagtt ggtagccgcc tgatagtgag
     2641 acagaatcgt ggcggcgctc agcgcattgg tgtaataagc cagttcatca atgttgccat
     2701 cgaaaccgaa actgatgtcc gaccggcagc caacgctcaa acctccagtt ccaccgctac
     2761 tcggcacata gctgggcagg acggtgggac cggaaacaag cgcgccgttc aggtaaccag
     2821 acacgttggt ggtgccgccg gacacatcca ccaccacggc caaataattc cactgatcac
     2881 gcgcgataga gccgggtgcg ccgatgttga aggacaggga aggggtggag ccgttctgat
     2941 tatatgcgcg aaacgtgtat tggccggggt tggaaatatc catgtaaatc agccagccgg
     3001 agcggggaga attgccctcg aaatcggagg tcgggcattc ggtggaactg atattccggg
     3061 ccaagaacca gccctcgatg gtgaatggag gtccgctgga caaagtcgcg tcataagcag
     3121 ccgtggcgta actcgtgccg ccattgaaat aatcagatga gtcggcgggt tgactggcaa
     3181 gagggctggc gttgccgaaa ataacatcgc cggcataatt caaattgagc gaggtatcag
     3241 tgccataatt ggtaatggtt gaacccgccg ccgcaggtgg cgtggcgact tcattcatgg
     3301 gatagtaggc agcgggatgc aagcccatca ccacgttgga atattgcgcc tggaccgtca
     3361 ttccggcact cattgccagg aacgccgacg ccatgcaggt taataggtgt tttcgcatag
     3421 gctttctgtc tttgtgttgg ttgttgtggt tttctaaagc aatcggggct ggtcccgttt
     3481 tgaaatcttg ctgtgtccgt ccgacatgct cttaaacgca aagcaaaccg gctttgggta
     3541 atcagcggta ttgccattca atattttcga aaaatgatgg agctgatggg ttatgcaaag
     3601 ttatccagtt taaaggaata actttcacta aattgaacat cgtttgcctc aaaaggcttc
     3661 gcaccttttg ggagcaaacg aatttttata tcaaacaatc tggtgccgtt ccgcctttga
     3721 aggaagtcga atctgcttgt ccgaaaataa aacaagttcg attttactct gcaggaacct
     3781 gtctcaagtt ggcaggtggc ggagcgatgg ttgcgcctgg gacatactcg agatggacgc
     3841 gcaccgattt ctgacttact tgtccgttgt ccactaattc catcacgcgg tgtccgatat
     3901 gccgcgcgag cacctgcgag ttacaacggt aaaaacatgg tggaggatgg aattcggcaa
     3961 agcaactgtc attggcgatg gcgaccagcg aaatgtctcc cggagtgcgg atccccttcg
     4021 aagccagcca cgaataaccg gtaagcagtt gtgccgagtt ggtgagtacc agtccggtcg
     4081 gacgttgttt caagctgaag atcagttcat aagcccgggc cacggaggcc ggcgaacggt
     4141 cctctttcaa aatgacgagc tttcccttcg tgccgatggt cgccagcgcc cgtcgcaaac
     4201 cgcgctccgc cgctacagtc ccgggactcg gatgctcgtt ttccatgatg ccgatgatgc
     4261 gatgcccatg acgcagcaac tggatccccg cgtgaaaagt cccttcttcc caatcattga
     4321 cagtgaatgg caaattgatg ccggtaaatg gcgcgccata aatgaaggca ggaatgttcc
     4381 tctcgtcaaa ccatcgctgc accatttcgc cggtggaata gagtatccag gctgccgatg
     4441 gttgctggcg caccagtcgc tcaagcaggc ggtccggatg cgccagatga aaaatgttcg
     4501 gggaagcaaa tcgcaagtcg cgcccctgtt tcgtgaggcg catctgcaag tcttccatct
     4561 ccagcaacac ctgccgatcc acaatccgtt cgggcgacaa aaaagtcacc ggaagccgcg
     4621 cggagtttgc tctcgtcgtg gaaggaacgt ggccgatttg cacctggcgt tgacgtcccc
     4681 ggctggttgg agttgtccat ccttcgcgct caagcaatct taatgcaagc cggagcgtgt
     4741 cgcgcccgac acccaaccgc attttgagcg gcaactctcc tggcaatgtg ccgcgcagaa
     4801 tccccgcttc aatccaatct ttgagcgtgt tggcggtttc gcgcaccaag ctcgttcgtt
     4861 tgggcaggcc atccattttt gtcaacggct caaaattatg cacgggttgg ccttcgcatt
     4921 tgggtactgt tcaaaaccgc cacgtgaacg aagccaggat cgaatgcgaa tagatcgggt
     4981 ccgaattggc gaacaagaat gagttgcctt caaaccaggt gtggctgagt gtgccgagaa
     5041 atttgtagct taaatccact tccatgttcg gagcgaatgc gtaactcacg ccgactttgc
     5101 cctgataagc gaacgtcgca tcggtatcat acgagtggct tctatcgccg acgttgtccg
     5161 tttgcgtgag atccaattca ctcgccactc cgccgcaccc cgcgccgacg taaatcgaga
     5221 caccattgtt tagcggcgcg cggaaaacaa tgtttgccag aaaaggaatt tgatacaggt
     5281 cggctcgttc gcccaaggag ctgaaggaaa ctccatcaac tttgtccatg cggttccaga
     5341 gcgtgccggt ttcgaattca actgcaattt ggtctgcgac ctggcagccg aaaataaaat
     5401 caccgcggac gcccgggtcg aagctgacgg ttgaaccggt gttcctgatt ttcaagtcct
     5461 gaacaatcac accgccaacg tccacaccga gataaaatgg tcccatggtg taatatggct
     5521 gatacggatt gtaaggctgg taaggattga ccgggacata ttgggcctgc aattttgtga
     5581 cggcaatcag gcacagcgcc atcatggccg acatgcgacg aatcattgtt ttcatagttc
     5641 ttcagagctt tcttttttcc ttgagacgca cacatgaacc gggcaaagga tttccacaat
     5701 ccttgcacca ttcggtgctc aaatattccg tgaatgcgca ttttgtcaat agcggcggct
     5761 ttggctagtg ggagtaacac taccggcatt tttgttgcca atcagtcgct ctttcgcatg
     5821 atatattgat tcacgatgaa aaatgttttt gatcttatca agggtgcaat ggcgtgtctc
     5881 acgttgttgg tggtgttggg aatggaaaca aaagccgtgg cgcaagacta ttcaggcgaa
     5941 gcaacggttg tccgcctgcg tggcaaggcc cgcgtctcaa gcgacggcaa gacctggcaa
     6001 acactcaaga agggcgcgca tgttccaaca ggcgccatga ttcaagcagc ggaaaattcg
     6061 acattggatt tggcgcttgg cgcagcggga ggccgctctg tttccggtga accggcagaa
     6121 ggcgttctgc gtttggaacc cgacacattg ctgaacatcg accatttggg cgcgggaacc
     6181 ggcgctgcgc aggacgtgga actggatttg cgcgccggac aaatcgcggg gaacatcggg
     6241 cgattgccat cgggatctcg gtatgagatc aaattttcca agggtgtcgc cggtgtgcgc
     6301 agcgggattt ttcacataaa cgcttccgga gtcgtcgctg tgccggctgg tgaaaccgtg
     6361 gtggttatcc tgggcgcgga tggcaatccc atcaccacca ttgtgaacac cggaaaagaa
     6421 ttcgaccctt cgacgggaat gatgaaagac atttccaaat cttcgagcgc gacaaccgcc
     6481 gcgcccccgg acagagcaga accgcctccc gctgctggtg caccgaaccc cacgccaaga
     6541 ggccccggca tgggcggttc cttgcgcaag ttttaagttg agctgaaatc tctgccgccc
     6601 gcttctttgg cgaagcgaca caaggcgaca cagcgtgtcg aacaaccgac gccgcgggtt
     6661 tacaaccact tacaactcgc gacaaccgat gctcgatgcc ggtatctgga tgctgattgc
     6721 tcgaccttac cattaaacca ataaaccttg agctttgaac ttcccgcagg ggagggggcg
     6781 gttttgcgcg ggatgatgcg ggacgacccg ggactatacg ggatttcaat cacttacaac
     6841 gtgaactcga tgctggatgc ttgatgctgg attttggagt gcgcggacat gtccacgctt
     6901 tggattggcg acatgtcgcc caaaagaaag cggcgtcgtg ccgccgcagt cccaaaagca
     6961 tcggaactcg gaacgatttt agaatgtcaa agaacagccc gtcgtcatgg agagacggga
     7021 atcagtcaaa atccgcaaag gcaggaacgg tgtattgccc gtgtagaaac accgcccgtc
     7081 cgccaactcg gaacccggaa cccggaactt gtttgccttc catcattcag tctcccatat
     7141 ttcagtttca actatcatgc ggaccatttt accacgcgat gtcaagcctc ccataggtat
     7201 cagcaggcgg cgtcccgccg acgaaacttg ccaccttacg cccgtttcct ttatcatgcg
     7261 tttgaacacg cacaggcact tggaattatg aaacttagat tgcttttggc cgccggcttc
     7321 gcggcgggtt ggctggcagc gtttgcgccg gaacaatcga gcgcggaaaa ttgggcgcat
     7381 tggcgcgggc cgctcttcaa tggctcaacc accgagacag gcctgccggt gaattggagc
     7441 acgaacgaaa acatcgcatg ggtcgcgccg ctgcccggct tgagcggggc cacgccaatc
     7501 gtctggggcg accgcgtgtt catctccagt ccggatgcgg acaaaaatct tttgctgctc
     7561 tgcttcaatc gcaaggacgg aaaacttctg tggcaaaaga ccgtcgccgt cggcgaccgc
     7621 accaaaggcc gcaacaacat ggcttcgcca tcgccggtga cggatggcaa aaaagtcttc
     7681 gtgattttcg gcacgggcga tcttgcggcg tatgatttcg acggcaacga attatggaaa
     7741 agaaatctcg gcaaggaata cggccgcttt gcgaacatgt ggctgtatgg ttcgagcccg
     7801 ctgttgttcg aggggaaact ttacgtgcag gtgctcgagc gcaatccgcg gccggatgat
     7861 tacacctccg cgatcgatgc caaacccgag cgcgaatcgt atctgctctg cattgagccc
     7921 aagaccggca aaaatatctg gcgtcatgtg cgtcccaccg acgcgaaaaa tgaatcgcag
     7981 gaggcgtatt cctcgcccat ccccgacacc ggcggcaaac acgcggaaat catcatcgtg
     8041 ggcggcaatt acaccacggc gcacgacccc
//
LOCUS       DASZMM010000181         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3915881, whole genome shotgun sequence.
ACCESSION   DASZMM010000181 DASZMM010000000
VERSION     DASZMM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3915881"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VG754_04360"
     CDS             <1..891
                     /locus_tag="VG754_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF18471.1"
                     /translation="SGRLSTVWQIVSFIPMIAGTFASGWITNHLPPKQTFLIVAGLSL
                     LIALYGLWKPRSVFEGAYNKPIAKATDFVGDIKRLLKHKPIYPAVIIMFLFQFAPGSN
                     TPLQFFLTDPHGQVRATDDAYANFNGVFQSAFIPVFFLYGWLCKRVSLKKLLWWGMII
                     TVPQYIPFLFIHSANSALFMAVPIGLMGGIAGVAIYDLAIRSCPPGLQGTLMMMVEGV
                     NILSTRGGDVLGTAIYNKFPKNPEIGFLYCVMAITAVYALNVVVLLMIPKEVIATADG
                     QSNPAVERKVLDEVAETEPT"
     gene            978..1700
                     /locus_tag="VG754_04365"
     CDS             978..1700
                     /locus_tag="VG754_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWF18472.1"
                     /translation="MGAQWKQAGRVANATKRGALIGKLVKEILVAAKLGDPHPENNPR
                     LWVAVEAAKKQSVPRDTIERAIKKGAGLLDEKVNYELVTYEGFAPHKVPVIVECLTEN
                     RNRTAPEIRHLFKDGQFGGPGSVAFMFDRWGIVEAHHADKSQDIEAAAIEAGAQEVQP
                     LTGEDVPEGHVGARFLCLPGDLDAVAKFLAAAKWSVTTSEISYLAKNYVELAEPQRKE
                     VADFLTALDEHDDVHRVYAALK"
     gene            complement(1753..2001)
                     /locus_tag="VG754_04370"
     CDS             complement(1753..2001)
                     /locus_tag="VG754_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18473.1"
                     /translation="MKSKKTTPPPQLAKGQLWKTEKGHVEIMEVGKTLTHYRLFQNQK
                     RVPTSLGRIEMVQDYLKNNQAKLVKNERLEKTRAEAVR"
     gene            2173..2562
                     /locus_tag="VG754_04375"
     CDS             2173..2562
                     /locus_tag="VG754_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18474.1"
                     /translation="MKTISTYTHGVLDYIAGIILILAPNLFSFADLNGPPVTVARVFG
                     VIFVVQALFTNYELGIFRILPLRLHLFNDYVLSAILAASPWIFGFYVQPANIWLPHLV
                     IGCVYFVITAMTRTTSRLMHIEPEPEV"
     gene            2658..>3270
                     /locus_tag="VG754_04380"
     CDS             2658..>3270
                     /locus_tag="VG754_04380"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWF18475.1"
                     /translation="MNAEEMESTVRSLIAEGKGILAADESLGTIEKRFQTINLPNTEE
                     NRRAYRELLFTTPKLGQYISGVILFDETIRQKTRDGVPFVEVLREQGIVPGIKVDKGA
                     KALAGFPGEKVTEGLDGLRARFKEYYELGARFSKWRAVITISEGIPTDACIHANAQAL
                     ARFAALSQEAGLVPIVEPEVLMDGNHAIERCEEVTEKTQHLVFN"
BASE COUNT          724 a          908 c          863 g          775 t
ORIGIN      
        1 tccggtcgct tgagcaccgt ctggcagatc gtcagcttta ttccaatgat tgcgggaacg
       61 tttgcgtcgg gttggataac aaaccacctg ccgccaaagc agacgtttct gattgttgcc
      121 gggctgtcct tgctcatcgc gctttacggc ttgtggaaac cgaggtctgt ctttgaaggc
      181 gcctataaca agccgatcgc gaaggccaca gacttcgtcg gcgacatcaa acgcctgctg
      241 aagcacaaac cgatttatcc cgctgtgatc atcatgtttc tttttcaatt tgcgccgggt
      301 tccaacacgc ctcttcaatt ttttctaacc gatccccacg gccaggtccg cgcgaccgac
      361 gatgcgtatg caaatttcaa cggcgttttt cagagcgctt tcatcccggt gtttttcctt
      421 tacggctggc tttgcaaacg cgtctcgctc aaaaaattgc tttggtgggg gatgatcatc
      481 acagtgccgc aatacatccc cttcctcttc atccactctg ccaactccgc gctgttcatg
      541 gccgtgccaa tcggtttgat gggcggcatc gccggggtcg cgatttatga tctcgccatt
      601 cgctcctgtc cgcccggcct gcaaggcacg ctcatgatga tggtcgaagg agtcaacatc
      661 ctgtccacgc gtggcgggga cgttttggga acggctattt acaataaatt tccaaaaaat
      721 cccgagatag gttttctcta ttgtgtcatg gcgatcacgg cggtttatgc attaaatgtg
      781 gtggtgctgc tcatgattcc caaggaagtc atcgcaaccg ccgatggcca gtccaacccg
      841 gcagtcgagc gcaaggtttt ggacgaagtc gctgagacag aaccgacctg ataatggccg
      901 aaatatcgct ccgaatcccc cgcaatccgt ctcgtttgct tgcactcacc ttctgttgcc
      961 gataaacttc tcccctcatg ggcgcacaat ggaaacaagc cgggcgggtg gccaatgcca
     1021 ccaagcgcgg agcgttgatt ggcaagctgg tcaaggaaat cctcgtggcc gcgaagctcg
     1081 gtgatccgca tcccgaaaat aatccgcgtc tttgggtcgc cgtcgaagcc gccaaaaaac
     1141 agtccgtccc gcgcgacacc atcgaacgcg ccatcaagaa aggcgcgggc ttgctggacg
     1201 aaaaggtgaa ttacgaactg gtgacttacg agggttttgc gcctcacaaa gtcccggtga
     1261 ttgtcgaatg cctgaccgag aaccgcaacc gcaccgcgcc ggaaatccgg catttgttca
     1321 aggacggcca gtttggcggg ccgggcagcg tggcatttat gttcgaccgc tggggaatcg
     1381 tcgaggcgca tcacgcggac aaatcgcagg acattgaagc cgccgccatt gaagcgggcg
     1441 cgcaggaggt gcagccgttg accggcgaag atgttccgga aggccatgtc ggcgcgcgat
     1501 tcttatgcct gcctggtgac ttggatgccg tcgcaaaatt tcttgccgcc gccaaatggt
     1561 cagtgaccac ttcggaaatc agttatcttg cgaaaaatta cgttgaactc gccgaaccgc
     1621 aacggaagga agtcgctgat tttctgaccg cgctggacga gcacgatgac gttcaccgcg
     1681 tctatgccgc gttgaaatga tcgcggagca caacatcaat ccaggcaact cagttccagc
     1741 aaacagccag tcttaacgca ccgcctcggc ccttgttttt tccagccgct cgtttttcac
     1801 aagctttgcc tgattattct tcaaataatc ctgcaccatt tcaattctgc ccaggctggt
     1861 tggaacacgc ttttggttct gaaacagccg gtaatgagtc agggtcttgc caacctccat
     1921 gatttcgacg tggccctttt ccgttttcca aagctggccc ttggccagtt gcggaggagg
     1981 cgttgttttt ttggacttca taaaaaatgc acccgtgccg gcggctttcc cgtccgacac
     2041 ggacacaatc taccacaaat cgcaaaagtt attaaactta tttaaatacc ggctttcacc
     2101 caaagaaaca ggggcgaaaa cgtaggtgtt ctccccggtt tgaccccgcg cattccggtt
     2161 tgattttgaa tgatgaaaac gatttcaacg tacactcacg gggtgctcga ttacatcgcg
     2221 ggcatcatcc tcatactggc gccgaacctg ttcagcttcg cggacttgaa tggcccgccg
     2281 gtgacggtcg cgcgggtgtt cggcgtcatt tttgtcgtgc aagcgttgtt cacgaattat
     2341 gagttgggta tcttccgcat cctcccattg cggctgcatt tgttcaacga ttacgtcctg
     2401 agtgcgatac ttgcggcgtc gccgtggatt ttcggtttct acgtccagcc ggccaacata
     2461 tggctgccgc acctcgtcat tggttgcgtc tattttgtca tcactgcgat gacccgcacg
     2521 acgtcgcgac tgatgcacat cgagccggag ccggaggttt gatcgaaatt catgcaagat
     2581 ttcatctgga attctcgcgt tatatttggt taagattacg cgggcacgga ttaacggaat
     2641 ttttgcaagg attgattatg aatgctgaag aaatggaatc cacagtgcgt tcactcatcg
     2701 ccgagggcaa aggcatcctc gcggcggacg aaagtttggg caccatcgaa aaacgttttc
     2761 aaacaatcaa tctgccgaac accgaggaaa atcgccgcgc gtatcgcgag ttgcttttca
     2821 ccacgcccaa gctcggccaa tatatcagtg gcgtaattct tttcgacgag accatccgtc
     2881 agaaaacgcg cgacggcgtg ccattcgtcg aagttctccg ggagcaaggc atcgttcccg
     2941 gcatcaaggt ggacaagggc gcaaaagcgc tggccggttt tcccggcgag aaagtcaccg
     3001 aaggcctgga cggtttgcgt gcccggttca aggaatatta tgaacttggc gcgcgcttca
     3061 gcaaatggcg ggcggtcatc acgatcagcg aaggcatccc aaccgatgcc tgcatccacg
     3121 caaacgcgca agcactcgcg cgtttcgccg ccttgagcca ggaagcgggc ctggtcccga
     3181 ttgtcgaacc ggaagtcttg atggatggaa atcacgcgat tgaacgttgc gaagaagtca
     3241 ccgagaaaac gcagcatctc gttttcaatg
//
LOCUS       DASZMM010000182         6913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1178645, whole genome shotgun sequence.
ACCESSION   DASZMM010000182 DASZMM010000000
VERSION     DASZMM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178645"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VG754_04385"
     CDS             complement(<1..1974)
                     /locus_tag="VG754_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18476.1"
                     /translation="MTIGAVFLRITMKMCARNKIFFGLLMFIGLPALLCGAQPSQKLP
                     GLQEAVLSKQDVYGNAALEQSNGPSYEFFHALIPSLHYVNADFAHYPLVLSAPSATRK
                     SRLVANGSAINARANTRAWNDFSAPALFRVGNDELAFGEYLQRLDGPKFADGWLPIVQ
                     FTYHHGAGEYFQETFASVEPSLASNAVSLTRFTYKAREKDESNGRMVVRFDTQEPLKA
                     EQGRILNGKGEVLAWFDSKWKWMAGRRVLEATLKNGAQAAIAVSTIPSSATIASPLDS
                     GGFDAQRQQCVETWSGILNRGMNIEVPEPYVNNAWRSLLVANFALINGDKMYYSAGNG
                     YDKLYEQEGGEAALALMQYGYEDDMRRLLPPLLDFTRKGLEFHQAGHKLDDICQYYWQ
                     TRDANFVNELRPRWQKEVDRLANHRSDANGLYPREQYCGDIATPVFSMNSNAKGWRAL
                     RDSAALLDALGDKTEAEHLRKICDDFRKDILAAADKSVRKDSDAAFVPNALLGEEQPY
                     DFITATKLGSYWNLMANDILATEVFGQQSELETGMCRYFQEHGGLFMGLTRSRPWPAF
                     WVSSANMNPLYGWYYVRTLLRRDEPDRALVSFYGMLAAGLTTDTFVCGEGCSIEPVDQ
                     WGRQFYLPPNSAGNAFFLQMLRNLLVQDWDLNED"
     gene            2242..3408
                     /locus_tag="VG754_04390"
     CDS             2242..3408
                     /locus_tag="VG754_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HWF18477.1"
                     /translation="MSDKQAPHVALGARCTITIQDIAFGGEGVGRIDDFVVFVPFVLV
                     DEVVEVEITEVKKRFARGRLLNVLTPSPERVQPQCRYFGDCGGCQYQHIDYAAQLRLK
                     HKQICDLFERIGGIKSEVIAPVVPCPQPYGYRNRIMIRSQWDKFKQGLNIGFIRADNR
                     LVVDIEECKIAEPALNEQIQYVRAHPPPKGGIKVVLRVAAENWEVPKDSFFQNNFFLL
                     PKLVETVRERLRESGNRFLVDVYCGVGFFGIEVADLVEAFAGVELDQMAIKAARRNAA
                     ARNLTNGEFLTGRAEEMLPELMRRFPAVKTTVVLDPPRTGCPPESLTLLQEVKPKQVI
                     YVSCHPATMARDLNVLCSKGVFDLLRVIPLDMFPQTQHVECVADLRLKPEALST"
     gene            3449..6208
                     /locus_tag="VG754_04395"
     CDS             3449..6208
                     /locus_tag="VG754_04395"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18478.1"
                     /translation="MNSTLAVQKDEMTTQFETPRVRGFVPSMWPWVLGAVMFGVFLVT
                     LSPWVTPYNLGEVLDLANWNQNSEAFGPVTFIITAPLRWLSPGRIPLALNLFSAFCGA
                     LALVTLARSVALLPHDRTHEQREREDSEFSILTMWASWLPPLFAVLVCGLQLTFWQQA
                     TIGTTEMFNLLIFAYVIRCLLEYRISGEESWLTKFAVVYGLGMANNWAMVGFFPAFLA
                     AMIWIKGLGAFSPRFIIRTFCLGLAGVSVIFLLPVLNKVFHGSPQTYWQMLRALLSAD
                     KKILTVFPKDVVRVLALTSLLPVFVMGIKWASYFGDNSPLGIFLATSMFHIVHALFLL
                     TCLWVALDSPISPRQMGFGIPFLTFYYLGALAIGYLSGYFLLVFGVEKSRHPVHPIVR
                     ALKFSVVAGVFLLLIGGPALMLRKNLPHVEAIKKGTQFYENYINEVARLLPSKGAVVL
                     SDDSFRLGYLQAALCRNEGHTPHLLVDTARLEQEPGYLRYLEKSNPGYQLSGPWTNIP
                     PDAIPVLGKVRVLQHLAATHPIYYMHPSFGYYFEGFYAEPHGLIYQLKPYPEGTWDVP
                     ALSKTLIAENQLYWKKLYDEVLPPLIDYVAKPSLPPTSTPWKKTLDKLHLKPEVETLA
                     FPAAVYYARVLDEWGVQLLTCGLRDEADKCFDEVVKLNPRAISARVNKNLDESLAAGK
                     KLTIRPAKEIEKSLEQYSGGWTELLRTDGPIDDPSFRSELGLTFATGWNSRQAIQEFH
                     RVRTLVPDDARISLQLAQLFIYVQDHTNGLSLLLPYSQGYSLALSNIDNVLKSYPNLP
                     TALFMKSYTLMQMKSYEQAIDPLTHYLANTKETNDYRGHLNRAIAYYKMGNYDAAKSD
                     YEVVGRIDPKVYQVYYGLGEIAYLQKDKDAAIKNYQLYLTNAPPDTEEAKNVNARLKE
                     LKTGP"
     gene            6214..>6913
                     /locus_tag="VG754_04400"
     CDS             6214..>6913
                     /locus_tag="VG754_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF18479.1"
                     /translation="MRVTHVITRLIVGGAQENTIASVLGLQQKPGVEAHLISGNDTGL
                     EGSLANAFAHSPQTLTLIPELVRPVHPWKDLLAYRRLTGIFRMQKPDIVHTHSGKAGV
                     LGRLAAHKARVPVIVHTIHGPSFGTFQGGAANFVFRSAERRAGKVTTHFVVVADAMTL
                     QYLAAGIASPLQYTKILSGFSLQPYLSATNDIAVRARFGIAPDDIVIGKIARLFKLKG
                     HDDLFSIAPELVRRV"
BASE COUNT         1437 a         1906 c         2000 g         1570 t
ORIGIN      
        1 gtcttcgttg aggtcccaat cctgcacgag aaggttgcgg agcatttgca ggaaaaaggc
       61 gttgccggcg ctgttcggcg ggagataaaa ctggcgtccc cattggtcca caggctcgat
      121 ggaacaacct tcgccgcaga caaaagtatc ggtggtgagc cccgccgcga gcattccata
      181 aaagctgacg agcgcgcggt cgggttcgtc gcggcgcaaa agcgtgcgga cataatacca
      241 gccgtaaagc gggttcatgt tcgcgctgct gacccagaac gcgggccatg ggcgcgagcg
      301 cgtcaagccc ataaaaagcc cgccgtgctc ctggaaataa cggcacatgc cggtttccaa
      361 ttcggattgc tgaccgaaaa cttccgtggc gagaatgtcg ttcgccatca ggttccagta
      421 gctgccgagc ttggtcgcgg tgatgaaatc gtaaggctgc tcttcgccga gcagcgcgtt
      481 gggaacgaac gcggcgtcgg aatctttgcg aacgcttttg tcggcggcgg cgaggatgtc
      541 cttgcgaaag tcgtcgcaaa tttttcgaag atgttccgct tcggttttgt cgccaagcgc
      601 gtcgagcaac gccgccgaat cgcgaagggc gcgccagcct ttggcgttgg agttcatcga
      661 gaaaaccggc gtggcgatgt cgccgcaata ttgttcgcgc ggataaaggc cgttggcgtc
      721 ggagcgatgg ttggcgagac ggtccacttc cttttgccag cgcgggcgaa gttcgttcac
      781 gaaatttgcg tcgcgcgtct gccagtaata ttggcaaatg tcgtcgagct tgtggccggc
      841 ctgatgaaat tcgagacctt tgcgggtgaa atcgagcagc ggcggaagca atcggcgcat
      901 gtcgtcctcg tatccatatt gcatcaaggc gagcgcggct tcgccgcctt cctgttcgta
      961 aagtttgtcg taaccgttcc cggcgctgta atacattttg tcgccgttga tcaatgcgaa
     1021 attcgcgacg agcaacgaac gccacgcgtt gttcacgtaa ggctcgggca cttcgatgtt
     1081 catgccgcga ttcaaaatgc ccgaccacgt ttcgacgcat tgctgccgtt gcgcatcgaa
     1141 accgccggag tcgagcggcg aggcgattgt tgcggatgat ggaattgtgg aaacggcaat
     1201 cgcggcttgc gcgccattct ttaaagtggc ttcgagcacg cggcgtccag ccatccattt
     1261 ccacttggag tcgaaccacg cgagaacttc gcccttgccg ttgaggatgc gcccttgttc
     1321 ggctttgagc ggttcttgcg tgtcgaaccg gacgaccatg cggccattcg attcatcctt
     1381 ctcgcgcgcc ttgtaagtga aacgcgtgag cgaaacggcg ttgctcgcca gcgacggttc
     1441 gacgcttgca aacgtttcct gaaaatattc gccggccccg tgatgatacg tgaattgcac
     1501 gatgggcagc cagccgtcgg caaacttcgg gccgtcgagg cgttgcagat attcgccaaa
     1561 ggcaagttcg tcgttgccga cgcggaaaag cgcgggagcg ctgaaatcat tccacgcgcg
     1621 ggtgttggcg cgggcgttga tggcgctgcc gttggcgacg aggcggcttt tgcgcgttgc
     1681 ggacggcgcg ctcaagacca gcggataatg tgcgaaatcg gcattgacgt aatgcagcga
     1741 agggataagc gcgtggaaaa attcgtagct ggggccgttc gattgctcga gcgcggcatt
     1801 gccgtaaacg tcctgcttcg aaagcacagc ttcttgaagg cccggcaatt tttgcgatgg
     1861 ctgcgcgccg caaaggagag cgggcaagcc gatgaacata agcaatccaa aaaagatttt
     1921 atttcgcgcg cacatcttca tcgtaattcg caggaacacc gcgccgattg tcatgcggcc
     1981 acgcgcggag taaagcaaat tagctgagtt actgaatact ttgcgggagt tacaacacat
     2041 ctcgcaaaca gtggagcgcg gctgtgcgcg aagcgccagc cgcagcaagc catccaaatc
     2101 ctgagtttcc tggctgctgc gactggtact gcggacacag tcgggttccg ttgcgattta
     2161 tgagataact tgtaatgttc gtccggtctt gacttcccgc gtttcggcct tagttcttaa
     2221 cgcggtgaat ccatgaatga aatgtccgat aaacaagcgc cgcacgttgc cttgggcgcg
     2281 cgttgcacga tcacgataca ggacatcgcg ttcggcggcg aaggcgtagg ccggattgat
     2341 gattttgtcg tgttcgttcc gttcgtgctg gtggatgaag tcgtcgaagt ggagatcacc
     2401 gaagttaaaa aacgttttgc gcgcgggcgc cttttgaatg tgctcacgcc ttcaccggaa
     2461 cgcgtgcaac cgcaatgccg ttacttcggc gattgcggcg gctgccagta tcaacacata
     2521 gattacgccg cgcaattgcg gttgaagcac aagcagattt gcgacctgtt cgagcgcatc
     2581 ggcggcatca aatcggaagt catcgcgccg gtggtcccgt gcccgcagcc ttacgggtat
     2641 cgcaaccgca tcatgattcg cagccaatgg gacaagttca agcagggttt gaacatcggg
     2701 ttcatccgcg cggacaaccg gctggtggtg gacatcgagg aatgcaagat cgccgagcct
     2761 gcgttgaacg agcagataca atatgtccgc gcgcatccgc cgcccaaggg cggcatcaag
     2821 gtggtcttgc gcgtggcggc ggaaaattgg gaggtgccca aggattcgtt ttttcaaaat
     2881 aattttttcc tgctgccgaa gctggtcgaa accgtccgcg aacgcctgcg cgaaagcggc
     2941 aaccgttttc tggtggatgt atattgcgga gtcggttttt ttgggattga agtcgcggat
     3001 ttggttgagg cttttgccgg agtcgaactg gatcaaatgg caatcaaggc cgcccgacgg
     3061 aatgccgcgg cgcgcaatct caccaacggg gagtttctca ccgggcgcgc ggaggaaatg
     3121 ttgccggagt tgatgaggcg ttttcctgcg gtcaaaacga cggttgttct cgatccgccg
     3181 cgcaccggat gccctcccga gagcctaact ttgctgcagg aagtcaagcc caaacaggtt
     3241 atttacgtct cctgccaccc cgcgacgatg gcgcgggact tgaacgtttt gtgctcaaaa
     3301 ggtgtctttg atttattacg cgtgatacca ttggacatgt ttccgcaaac gcaacacgtc
     3361 gagtgtgttg cggatttgcg gttgaaaccg gaagctcttt caacttagac tccgttcctg
     3421 acacctttag cttgccaaac gcatgggcat gaattcaact ctggccgttc aaaaagacga
     3481 gatgacaact caatttgaga cgccccgcgt ccgggggttc gttccttcga tgtggccatg
     3541 ggtgttgggc gcagtgatgt tcggcgtttt cctggtcacg ttgagcccgt gggtcactcc
     3601 gtacaacctg ggcgaagtgc tggacctggc gaattggaac cagaactccg aagcgttcgg
     3661 gcccgtcact tttatcatca cggccccgct gcgctggctg tcgccggggc ggattccgct
     3721 ggcgttgaat ttgttctcgg cgttttgcgg cgcgctcgcg ttggtgaccc ttgcgcgatc
     3781 agttgccctg ctgccgcatg accgcaccca cgaacaacgg gaacgggaag atagcgaatt
     3841 ctccatcctc acgatgtggg cgtcatggtt gccgccgctt ttcgcggtgc tggtttgcgg
     3901 gcttcaattg actttctggc aacaggccac gatcgggacg accgagatgt tcaacctgct
     3961 catattcgcg tatgtcatcc gctgcctgct cgaatatcgc atcagcggcg aggaatcgtg
     4021 gctcaccaaa tttgccgtcg tgtatggact tggcatggcc aacaattggg cgatggtcgg
     4081 tttcttcccg gcgttccttg cggccatgat ctggataaag ggactcggcg cattcagtcc
     4141 gcgcttcatc atccgcacgt tctgcctggg gctggccggg gtttccgtca tatttctgct
     4201 gccggtgctg aacaaagttt tccacgggtc gcctcaaacc tactggcaga tgttgcgggc
     4261 gttgctttca gcggacaaaa aaatcctcac tgtttttccg aaggacgttg tcagggtgtt
     4321 ggcgctgact tccctgctgc cggtttttgt gatgggcatc aagtgggctt cttatttcgg
     4381 ggacaacagt ccgctgggaa ttttcctggc gacaagcatg tttcacatcg tccatgcctt
     4441 gtttctgctg acctgccttt gggtggcgct ggactcgccg atcagcccgc gtcaaatggg
     4501 cttcggcatt cccttcctga cgttctatta tctgggggca ttggccatcg gttatttgag
     4561 tggatacttt ttgctcgtct tcggagtgga gaaatcgcgg catccggtcc atcccatcgt
     4621 gcgcgcgttg aaattttccg tggtcgcggg cgtctttcta ttgttgatcg gcgggccggc
     4681 gttgatgctt cgcaaaaacc tgccgcacgt ggaagcaatc aaaaagggca cgcaatttta
     4741 cgagaattac atcaatgaag tcgcccggtt gctcccctcc aaaggcgcgg tcgtcctaag
     4801 cgacgactcg tttcggctgg gttatctgca ggcggcgcta tgccgcaatg aagggcacac
     4861 tccgcacctt ctggtggata cggcccggct ggagcaggag ccgggatatc tgaggtatct
     4921 ggaaaaatcc aatcccggtt atcagctttc cgggccatgg acgaacattc cgcccgatgc
     4981 cattcctgtg ttgggcaaag tccgggtgct tcagcacctc gcggcgacgc atcccatcta
     5041 ttacatgcat cccagtttcg gatactattt cgaaggattt tatgcggagc cgcacgggct
     5101 gatttatcaa ctcaagccat acccggaagg aacatgggac gtgccggcgc tttccaagac
     5161 gttgatcgcg gagaaccagt tatactggaa aaaactttat gatgaagtcc tgcctccatt
     5221 gatcgactat gtggccaagc cttctcttcc accgacttca acaccatgga aaaagaccct
     5281 cgacaagttg catctgaaac cggaagtcga aacgctggcc ttcccggcgg ccgtttacta
     5341 cgcgcgcgtc cttgacgaat ggggtgtgca acttctgacg tgcggtctcc gcgacgaagc
     5401 cgacaaatgt ttcgacgaag tggtgaaact taatccacgg gcgatttccg cgcgtgtgaa
     5461 taaaaatctc gatgaaagcc tggctgcggg caaaaaactc accatccggc ccgcgaagga
     5521 aatcgagaaa agccttgaac aatacagcgg cggctggacg gaattgctcc ggacggacgg
     5581 gccgattgat gatcccagtt ttcgcagcga gcttggcttg accttcgcca ccggatggaa
     5641 cagtcgccag gccatccaag agttccaccg cgtccggacg ctcgttcccg acgatgcgcg
     5701 tatatcgctt caattggcgc agcttttcat ttacgtccag gaccacacca acggtctttc
     5761 gctgctgctg ccgtattcgc aaggttattc cctggcactt tcaaacattg acaacgtgtt
     5821 gaaatcgtat ccgaaccttc ccactgccct gttcatgaag agttacacgt tgatgcagat
     5881 gaaatcctat gagcaggcca tcgatccgct gacgcactat ctggcgaaca ccaaggagac
     5941 gaacgattac cgaggccatc tcaaccgcgc catcgcctat tacaaaatgg gaaattacga
     6001 cgcagccaag tcggactacg aagtcgtggg ccggatcgac ccgaaggttt accaagtcta
     6061 ttacggcctg ggtgagattg cctacctgca gaaggacaag gacgcagcca tcaagaatta
     6121 tcagctttat ctcaccaacg cgcccccgga cactgaagag gccaaaaacg tcaacgcgcg
     6181 cctgaaggaa ctcaagaccg gcccttagca cggatgcgcg tcacgcacgt catcacccgg
     6241 ttgattgtcg gcggcgccca ggaaaacacc atcgcgtcgg tccttggtct gcaacaaaag
     6301 ccgggggttg aagcccattt gatttccggc aacgacaccg ggctggaagg ttcgctcgcc
     6361 aatgcctttg ctcattcccc gcaaacgctc actctcattc ccgaattggt gcgtccggtc
     6421 catccgtgga aagatttgct ggcttatcgg cggctgaccg ggattttccg gatgcaaaaa
     6481 ccggacatcg tccatacgca cagcggcaag gccggcgtgt tgggacgact ggccgcgcac
     6541 aaagcgcgag tgccggtgat tgtccatact atccatggcc cgtcgttcgg gacctttcaa
     6601 ggaggcgcgg caaattttgt gtttcgctcc gcggaacgtc gcgcggggaa agtgaccact
     6661 catttcgtcg ttgttgccga tgcgatgacg ctgcaatatc ttgccgccgg catcgcttcg
     6721 ccccttcaat acacgaaaat cctcagcggc ttttccctgc aaccgtatct ctcggccacg
     6781 aacgacatcg cagtgcgcgc aagattcggc atcgcgcctg acgacattgt cattggcaag
     6841 attgcgcggc ttttcaaact gaaagggcac gatgaccttt tcagcatcgc gccggaactg
     6901 gtgcgccgcg ttc
//
LOCUS       DASZMM010000183         5276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_624036, whole genome shotgun sequence.
ACCESSION   DASZMM010000183 DASZMM010000000
VERSION     DASZMM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624036"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VG754_04405"
     CDS             <1..353
                     /locus_tag="VG754_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF18480.1"
                     /translation="ELIARVRSLGRRAGEPKSVILRIGDLSLDTITRQAQRGGKIFEL
                     TTREYRLLEFLMRTPGRICGRMAILEKVWDYDFDPGTNLVDVYIKRLREKIDDGFEPK
                     LLHTVRGVGYVMKE"
     gene            357..1424
                     /locus_tag="VG754_04410"
     CDS             357..1424
                     /locus_tag="VG754_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF18481.1"
                     /translation="MTIRARLTFWYAGILFVSVLLITGLSYHEFARERAKARPSNEES
                     DENDTPEDILQISMVCGIPAAIIALAGGWWLMRKAMAPVTALTDAASRINESNLNRQL
                     LRSGNGDELDRLTEVFNAMTARLDTSFNRIREFTVHASHELKTPLTVMHGELEIALQD
                     SALSANSREGLLSQLDEVQRLTKIVDGLSLLTKADAGQIVLKREPVRLDELARDCIAD
                     AQILAHPRQVQVELDRCEEIAIMGDRHRLRQLLLNLTDNAIKYNQSGGTLKLELCRAE
                     KFAELKISNTGPGIPAESLGRVFERFFRGDASHNNMVDGCGLGLSIAQWIVTAHDGSI
                     DIKSEAGKWTTVTVRLPVAER"
     gene            complement(1468..2820)
                     /locus_tag="VG754_04415"
     CDS             complement(1468..2820)
                     /locus_tag="VG754_04415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWF18482.1"
                     /translation="MKSANRILHVDGDSFFASCEVAKNPDLQGRPVWVGGGRKGDGIV
                     IAANRVAKSFGVKTGMACFEAARLCPRGVLCRPHYDTYRECSEKMFRILEEYAPVLVP
                     ISIDEGFLDFTTMDKHVWRHTTPLEYIKEIGERIHKDVGLPVSGGLANSSRLAKLATD
                     AAKPGFIEIPAGQEKEFLKDRPVRELSGIAKNRERALSALGARTFGDVTKLPSMLLKQ
                     KFGIWGQQLWLFAQGRWNEPLVLEANDRTSISSNTTLPSDEPDYEAALTFTLSETIRL
                     VGQLRREQLQARELGVAIRFKDFTEIGMNHRFKHPQFRNSVINAEVEKLFRELMSSAF
                     QPVRQIRIAFWNLSRLDIHPTLWGKTEAERWGALDEAAHQLNQQFARPEKPALMTGSQ
                     LALRQLDDAHKSPKAKCPFVPQREMIKKLWGTDADPLAHKGEVEKRLAKVSKQSQYHH
                     "
     gene            complement(2890..3960)
                     /locus_tag="VG754_04420"
     CDS             complement(2890..3960)
                     /locus_tag="VG754_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWF18483.1"
                     /translation="MNEKTLSTIASRLLRIPAAPYHETAVRAEVEKICGEHGLNCQRD
                     TYGNVLVRLQTVSRQRPFVLAAHLDHPGFEIIRPLAGKRWLARFLGGVGDTYFKKGIP
                     IRLMPGGVPAKLGKRVGKEKQFEIHALKNPSEPPRFAVWELEDFALRGGKIHARACDD
                     LVGVAAILATMISLQKTRARVNVIGVISRAEEIGFQGALTVADSRLLPKDSLVISLET
                     SRELPPAVMGKGVILRVGDRTSIFDSTAMRFLAEVAGDLNRRDKSFQFQRALMYGGTC
                     EATAYQEFGFQTGAVCVALGNYHNCAPNNKIAAEYVSLADACSMVDLLVAAAKQMKNF
                     RRLTARLPVRLKKLLKQARRKL"
     gene            complement(4041..>5276)
                     /gene="hemW"
                     /locus_tag="VG754_04425"
     CDS             complement(4041..>5276)
                     /gene="hemW"
                     /locus_tag="VG754_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWF18484.1"
                     /translation="SSEPVASLYVHVPFCAQKCNYCAFYSEASNGELINRYVEALLRE
                     MEIAAGDLRPRTIFFGGGTPSLLTLRQWEKILQTMQRLNLTGAAEWTVECNPATVSLD
                     KARLLRSYGVNRISMGVQSLNEALLDRLGRIHSREMVFKSFDILRAAGFENINIDLMF
                     AIPGQTLEVWRETLAEATALGSEHLSSYEVIYEDDTPLYAQLKAGEFDVDEDLACEMY
                     EELVEHTARAGLHQYEIANFARESRLPPATAIRTSSQIAKRPSNFELGTLNPELATAA
                     PSLACQHNINYWRGGSFYGLGPSATGYVRGVRTKNWSNTQLYCEQLEKGRRAIESSEE
                     LSPRARAGEIAAFGLRMNIGWPFEHFEQVTGRDLRREWSGEIEQLVARGWGSSNPRGF
                     RLTPAGLRFADAAAELFLR"
BASE COUNT         1161 a         1442 c         1546 g         1127 t
ORIGIN      
        1 ctgagttgat cgcgcgcgtg cgttcgctcg gacgccgggc cggcgaaccg aaatcggtca
       61 tcttgcgcat cggcgacctc tcgctcgaca ccatcacacg ccaggcgcaa cgcggcggaa
      121 aaatttttga actcaccacg cgcgaatacc ggcttttgga attcctgatg cgcacgccgg
      181 gccggatttg cgggcgcatg gcaattctcg aaaaggtttg ggactacgat ttcgatccgg
      241 gcacgaatct tgttgatgtg tacatcaaaa ggttgcgcga aaaaattgat gatggtttcg
      301 agcccaaatt gctgcacacc gtgcgcggcg tcggttacgt gatgaaggaa tgaaccatga
      361 ccatccgcgc gcgcctcacg ttttggtatg ccggcatttt gttcgtttcg gtgctgctga
      421 tcaccgggtt gtcgtatcat gaatttgcgc gggaacgcgc aaaggcgcgg ccttccaatg
      481 aggaatcgga cgagaatgac acgccggaag acatcctgca aatctcgatg gtctgcggca
      541 tcccagcggc aatcatcgcc ctcgcaggcg gttggtggct gatgcgcaaa gcgatggctc
      601 ctgtcaccgc gcttactgac gccgcatcgc gaatcaacga aagcaatctc aacaggcaac
      661 tgcttcgcag cggcaatggc gatgaactgg accgtcttac cgaggtgttc aacgccatga
      721 ccgcgcggct ggacacttcg ttcaaccgca tccgtgaatt cactgttcac gcttcgcacg
      781 aactcaagac gccgctgacc gtgatgcacg gcgaacttga aatcgcgttg caagacagcg
      841 ccctttccgc aaacagccgc gaaggtttgc tcagccagtt ggatgaagtc cagcggctca
      901 cgaaaattgt ggatggcctt tctttgctca caaaagcgga cgcgggacaa atcgttttga
      961 aacgcgaacc ggtgcggctg gacgaattgg cgcgcgactg cattgccgac gcgcagatcc
     1021 ttgcgcaccc gcggcaagtc caggtggaat tggatcgttg cgaagagatt gccatcatgg
     1081 gcgaccggca tcgcctgcgc caattgctgc tgaacctcac cgataatgcg atcaaataca
     1141 atcaatccgg cggaacgctg aagcttgagc tttgccgcgc ggaaaaattt gcggaactga
     1201 aaatctccaa caccggcccg ggcatcccgg cggaatcgct gggacgggtc ttcgaacgat
     1261 tttttcgcgg tgatgcgagc cacaacaaca tggttgacgg ctgcggcttg ggattgagca
     1321 tcgcgcaatg gattgtcact gcgcatgacg gttccattga catcaaatcg gaagcgggca
     1381 aatggacaac cgttacggtg cgattgccgg tggcggaaag gtaaaggcaa ttttcgcagg
     1441 agcccggcgc aagcaactcc aaaaccttca atggtgatac tggctttgct tgctgacttt
     1501 ggcgaggcgc ttttcgactt cgcctttgtg cgcgagcggg tcggcatcag tgccccaaag
     1561 ttttttgatc atttcgcgct ggggcacgaa cgggcatttg gccttgggac ttttatgggc
     1621 gtcatcgagc tggcgaaggg ccagttgcga gccggtcatc agggccggtt tttcaggacg
     1681 cgcgaactgc tggttcaact ggtgggccgc ttcgtcgagc gcgccccagc gttcggcttc
     1741 ggtcttgccc caaagcgtgg gatgtatgtc gagccgcgac aaattccaga acgcgatgcg
     1801 gatctgccgc acgggctgaa atgcggaact cattaattcg cggaataatt tttcaacctc
     1861 ggcattgatc acggagttgc ggaattgcgg atgtttaaag cggtggttca tgccgatctc
     1921 cgtgaaatcc ttgaagcgga tggcgacgcc cagttcgcgg gcctgcaatt gctcgcggcg
     1981 caactgcccc accaaccgga tggtttcgct caatgtgaag gtaagcgccg cctcgtaatc
     2041 cggttcgtca gaaggcagcg tggtgttgct ggaaatgctg gtgcggtcat tggcctccag
     2101 gaccagcggc tcgttccaac ggccctgtgc gaaaagccag agttgttgtc cccaaatgcc
     2161 aaacttctgc ttgagcaaca tggagggcag cttcgtcacg tcgccgaaag tccgcgcacc
     2221 caatgctgac aaggcgcgtt cgcggttttt ggcgatgccg gaaagttcgc gcaccggacg
     2281 gtccttgaga aattcctttt cctgtccagc gggaatttca atgaaaccgg gcttggcggc
     2341 atcagtggcg agcttggcaa ggcgcgagga attggccaac ccgcccgaaa ccggcaggcc
     2401 gacgtccttg tgaatgcgtt cgccgatttc cttgatgtat tccaagggcg tggtgtgccg
     2461 ccaaacatgc ttgtccatgg tggtgaagtc cagaaagcct tcatcaatgg aaatcgggac
     2521 gagcaccggc gcatattctt caagaatgcg gaacattttt tccgaacatt cgcggtaggt
     2581 gtcataatgc ggacgacaga ggacaccgcg cgggcaaagc cgggctgctt caaagcaagc
     2641 catgccggtc ttgacgccga aactttttgc gactcgattg gccgcgatga cgatgccgtc
     2701 gccttttcgt ccgccgccga cccagacagg acgtccctgc aagtccggat ttttagccac
     2761 ttcgcaactg gcgaagaagc tgtcgccgtc aacgtgcagg atgcggttgg cgcttttcat
     2821 ctccaagaca agtttaccac gggacaaaat gccgccaagc aacaaagaga aaattataaa
     2881 atggcatcct cataattttc gccgggcttg cttgagcaac tttttcaggc ggacgggcaa
     2941 tcgggcggtg aggcggcgaa agtttttcat ctgctttgcc gcggcaacga gcaaatcgac
     3001 catgctgcag gcatcggcga ggcttacgta ttcggcggcg attttgttgt tcggagcgca
     3061 attatgatag tttccaagag cgacgcagac agcgccggtt tgaaaaccaa attcctggta
     3121 ggcagtcgct tcgcaggtgc cgccatacat caaggcgcgt tggaattgaa aggatttgtc
     3181 gcggcgattc aggtcgcccg ccacttcggc aagaaagcgc atcgctgtgg agtcaaagat
     3241 ggaagtgcgg tctccaaccc ggagaatcac tccctttccc atgaccgccg gcggcaattc
     3301 gcggctcgtt tccaatgaga tgacaagaga gtctttcggc aatagccgcg aatcggcaac
     3361 cgtcaacgcg ccttggaaac caatctcctc cgcgcgcgaa atcacgccga tgacattgac
     3421 ccgcgcgcgg gttttttgca gcgaaatcat tgttgccagg atggccgcaa cgccgaccag
     3481 atcgtcacac gcgcgcgcgt ggattttgcc gccacgcaag gcaaaatctt ccagctccca
     3541 aacggcgaat cgaggcggct cacttggatt cttgagcgcg tggatttcga attgcttttc
     3601 tttgccgaca cgtttgccca gttttgccgg cactccaccg ggcatcaagc ggatgggaat
     3661 gcctttcttg aaataagtgt cgccaacgcc gccgaggaat cttgcaagcc agcgtttgcc
     3721 ggctaaagga cggatgattt caaaaccggg atggtccaaa tgcgcggcaa gaacgaacgg
     3781 acgttgccgg gagacggttt gcaggcggac aagcacattg ccataggtgt cgcgttggca
     3841 attcaagcca tgctcgccgc aaattttttc cacttctgcg cgaacggccg tttcgtgata
     3901 cggcgcggct ggaatgcgca aaaggcgcga agcaatggtg gagagagttt tctcgttcat
     3961 aagaaacatc gagcaaccga catcgaacgt tgaacgccca aattagacgc ttggaaattc
     4021 attggacgtt cagcctcgaa tcagcgcaga aataactctg cggcggcatc ggcgaaacga
     4081 agaccggccg gggtgagtcg aaacccgcgt ggattggaac tgccccagcc gcgggcaacc
     4141 agttgctcga tttcgccaga ccattcgcgg cgaaggtccc ggccggtgac ttgctcaaaa
     4201 tgctcgaacg gccagccgat gttcatccgc aagccgaatg cagcaatctc tccggcgcgc
     4261 gcgcgcggcg aaagttcttc gctggactcg attgcccgac ggcccttttc cagttgctcg
     4321 caataaagtt gtgtgttcga ccaattcttg gtgcgaacgc ctcggacgta acccgttgcg
     4381 ctcggtccaa gtccgtagaa agaaccgccg cgccaatagt tgatgttgtg ctggcaggcg
     4441 agcgacggcg cagccgtcgc aagttccggg ttcaaagttc cgagttcaaa gttggaggga
     4501 cgctttgcga tttgcgatga tgtccgaatg gcggttgcgg ggggcaatcg cgattcccgg
     4561 gcgaagttgg cgatttcgta ttggtgcagg ccggcgcgtg cggtgtgttc caccaattct
     4621 tcgtacatct cacaggcaag gtcttcgtca acatcgaatt cgccggcttt caattgcgca
     4681 taaagagggg tgtcgtcctc gtaaatgact tcgtaactgg aaagatgttc gctgccgagc
     4741 gcggtggcct cggcgagagt ttcgcgccag acttcgagcg tctggccggg aatggcgaac
     4801 atcagatcaa tattgatgtt ttcaaaacca gccgcacgca agatgtcgaa cgatttgaaa
     4861 accatctcgc ggctgtgaat gcgtcccaac cggtcgagca aggcctcgtt cagcgactgc
     4921 acgcccatcg agatgcgatt cacgccgtat gagcgcagca agcgggcctt gtccaatgac
     4981 actgtggcgg gattgcattc tacggtccat tccgccgcac cggtgagatt gaggcgctgc
     5041 atggtttgga gaattttttc ccattgccga agtgtcagca gtgaaggcgt gccgccaccg
     5101 aagaaaatcg tgcgcgggcg caaatcgcca gcggcgattt ccatctctcg aagcaatgcc
     5161 tcgacataac gattgatgag ttcgccgttg gaagcctcgg aataaaaggc gcaataattg
     5221 cacttctggg cgcagaaggg cacgtgcaca tacaaactcg cgactggctc tgatga
//
LOCUS       DASZMM010000184         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_69225, whole genome shotgun sequence.
ACCESSION   DASZMM010000184 DASZMM010000000
VERSION     DASZMM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69225"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2776)
                     /locus_tag="VG754_04430"
     CDS             complement(<1..2776)
                     /locus_tag="VG754_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF18485.1"
                     /translation="MKLFKPSWVGCLPYFSGPGYPPHNGREALVKEANEETIIIFSNG
                     HRPAYAVESEGRVLSQKLVASPIFKYAAAAGFVGLAALMGLSLNKVLQVQCLYASFFL
                     AVLAGAAFGGWKPGCLVIILSVLLVNRSFISSGGSNAFDADLPAKLIIFLAVSAVIVG
                     VVEMQRMTQRRSDALVRQLQLEIERQEQTEIQLAASEERYRSLFENNLDAVFTLDTEG
                     FFMTANPSALKLCGYSLEELQKVRFTGLCSPDQWTEAGRHFQLALQGTPEPFFEVPII
                     TKEGRRVDLFITGGSIKLKGKTVGIFAIAADVTERKRLQTRLAAFSQVGQRLSSVTTP
                     IAAGRIIGDVADELFGWDLFSLNLYSVESNQTDYVLNVDTFDGQKTEILPFLSAPGIP
                     ALAHRIIAHGAELVIDPSMLPGDAVPAKGARRSDSMMFVPINDKEKTIGVLSVGKCKP
                     DAYDEQDLKTLLALADYCGGALQRIRAEAGWHRNEQDYRALFDSDGSGKCQVDPRTGK
                     ILRVNPKFCELTGYSSHELMEKTVREITHPDDREVSGQNFERLRRSEIHEFRMEKRYE
                     RKDKSVVWVDTTVTLVHDPEGQPARVITSSVDITARVQAESALHESENRFTSFMQHLS
                     APAWMKDLEGRYLYVNAACANVIRQSPESILGRTDADLFPSEIARAFVRNDRRVLTSG
                     KALQTLETFHEQDGLHYSVINKFPILDKNGGAQIVGGIAMDVTERKAAENALVVSNQR
                     LNLIAQVTNAVVGDEPLAEQMRNVVKTVRDAYAVKACIIRVMEGEELALLAYAGMEPG
                     ALAPRVPVNCAISREILKRAGPLFIPNLRAFVPDPSSPPEPANAAGFVSYAGAPLLAQ
                     DKIIGVVGIYTEQPMADFGDTDLQHLQIIANHIAVAIANNRLYREIKLQKSFLEEQIR
                     EREQAEDEVQK"
BASE COUNT          730 a          939 c          856 g          733 t
ORIGIN      
        1 gtttttggac ctcgtcctcc gcctgctccc gctcgcggat ttgttcctcc aaaaatgatt
       61 tttgcagctt gatttcccgg taaagccggt tgttggcgat cgccaccgcg atgtgattgg
      121 cgatgatttg caggtgctgc aagtctgtat cgccaaaatc ggccatgggc tgctcggtat
      181 aaatgcccac cacgccaatg attttatctt gcgccagcaa cggcgcgccc gcgtaggaaa
      241 caaaaccggc tgcgttcgcc ggttccggag gcgaggacgg gtctggcacg aatgcccgca
      301 aattcggaat gaacagggga ccggcgcgtt tgagaatttc ccggctgatc gcgcagttga
      361 cgggcacccg gggcgcgaga gcgcccggtt ccatccctgc ataagccagc aatgccagtt
      421 cttccccctc catcacacgg atgatgcaag ccttgactgc gtaagcatcg cggacagttt
      481 tgaccacgtt gcgcatctgc tcggcaagcg gctcgtcccc gacgacggcg tttgtgacct
      541 gcgcgatgag gttcaaccgc tggttggaca cgacgagcgc gttttcagcc gccttgcgtt
      601 ccgtgacgtc catcgcgatt ccccccacga tttgcgcccc gccatttttg tccaggatgg
      661 gaaatttgtt tatcactgaa taatgcaggc catcttgctc gtgaaatgtt tccaacgtct
      721 gaagcgcttt cccggaagtg aggacgcgcc tgtcattcct gacgaatgcg cgcgcaattt
      781 ccgacgggaa caggtccgca tccgtccggc ccaagatgct ctcagggctt tgtcttatca
      841 cgttggcaca agccgcattg acgtaaagat agcggccctc aaggtctttc atccatgcgg
      901 gggcggagag atgctgcatg aaggaggtga accggttttc gctttcgtgc aacgcggact
      961 ccgcctgcac ccgggcggtg atgtccacgg agctggtgat cacgcgggca ggctggcctt
     1021 ccggatcatg cacgagtgtc accgtcgtgt ccacccagac aacggacttg tccttgcgtt
     1081 cgtaacgttt ttccatgcga aattcatgga tctcgctccg cctcaaacgc tcgaaatttt
     1141 gtccggaaac ttcccggtca tccgggtggg tgatttcgcg aacggttttt tccatcaatt
     1201 catggcttga atatccggtc aattcgcaga acttggggtt cacccgcaaa attttgccgg
     1261 tgcgggggtc cacctggcat ttgccgctgc cgtccgaatc gaaaagcgcg cggtaatcct
     1321 gctcgttgcg atgccaaccc gcttccgccc ggatgcgttg caatgcgccg ccgcaataat
     1381 cggccagcgc cagcagcgtc ttgaggtcct gctcatcgta agcatccggt ttgcatttgc
     1441 ccacggacag gacgccgatt gttttttcct tgtcgttgat cggcacaaac atcatcgagt
     1501 ccgaacgccg cgcgcctttt gccggaaccg catcaccggg cagcatggac ggatcaatga
     1561 caagttccgc tccgtgggcg atgatgcggt gcgccaacgc cggaatgccg ggagcggaca
     1621 ggaacggcag gatttccgtt ttttgcccgt caaacgtatc gacattcaac acgtaatcgg
     1681 tctggttgct ttcaaccgaa tacagattga ggctgaacaa atcccagccg aacaattcgt
     1741 ccgccacgtc gccgatgatc cgtccggcgg caatcggtgt cgtgacagaa ctgagccttt
     1801 ggcccacttg cgagaacgcg gcaagacgcg tctgcaaacg tttccgttct gtgacatccg
     1861 cggcaatggc aaagatgccg acggtttttc ccttgagctt gatcgagccg cccgtgatga
     1921 acaggtccac gcgcctgcct tccttcgtga tgatggggac ttcaaaaaaa ggttcgggag
     1981 tgccttgcaa agccagttga aaatgccggc cggcttccgt ccattggtct ggcgaacaga
     2041 gcccggtgaa gcgcactttc tgcaattctt cgagtgaata gccgcaaagt ttgagggccg
     2101 aagggtttgc cgtcatgaaa aagccctcgg tatccagcgt gaagactgcg tccaggttgt
     2161 tctcaaacaa cgaacgataa cgctcctcgc tggcggccaa ctggatttcc gtctgttcct
     2221 gccgttcgat ttcaagctgc aactgccgca ccaacgcatc cgaacggcgt tgcgtcatcc
     2281 gttgcatttc gaccaccccg acgatgaccg cgctcaccgc caaaaaaatg atcagtttcg
     2341 cgggaagatc cgcatcaaag gcgttcgacc cgcccgacga aataaaagaa cggttcacca
     2401 gcaaaacact caagatgatc acgagacatc caggtttcca acctccaaaa gcggcgccgg
     2461 caagcacggc gagaaaaaaa gaggcataca ggcattgcac ttgcagcacc ttgttcagcg
     2521 acagacccat caacgctgcc aatcccacaa agcccgcggc ggctgcgtat ttaaaaatag
     2581 gagatgcgac gagcttctga ctcaaaacgc ggccctcgct ttcaaccgca tacgcgggac
     2641 gatgaccgtt actgaaaatg atgattgttt cttcattcgc ttccttcacg agtgcctcac
     2701 ggccattatg gggtggatac cccggcccac tgaaataggg taaacacccc acccatgaag
     2761 gtttaaaaag tttcacgaag ctattaacca cggattcaac acaacacgta tgagagcgag
     2821 acatgctttg tagaaaacat ttctgttcat ggaaaaaaga acggaaccct tggccgtgac
     2881 gattcagttg aatggggagc gcacgcctct ggcgtgcagt gttcggcgtt cagcgttgtt
     2941 cgatgttttt aactcgccct ccacgcatga acatgaaaca tgaaacatcg aacgcccaac
     3001 atcgaacatc gaactctgaa ttgggaaaat accagacggc tctggaacat tggatgttcg
     3061 gcgttcagcg ttggttgttc gatgtttccg gactgcttct ccgctgcgtc cccgcatata
     3121 catgtcacgg attgtccctt gaccttgccc tttcaaagcg gtttactcgc acctgatcac
     3181 tgaaaatttg aattgctcac agaaagaaag aaaaatcaac atgcaaaaga agaatctcaa
     3241 tctccctgtt tccccggc
//
LOCUS       DASZMM010000185         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5014596, whole genome shotgun sequence.
ACCESSION   DASZMM010000185 DASZMM010000000
VERSION     DASZMM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5014596"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VG754_04435"
     CDS             <1..789
                     /locus_tag="VG754_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWF18486.1"
                     /translation="RLEELEERLNLLHSLKRKYGATVAEVIAFGGEAKRKLEQLEQRD
                     AELARLNGELKKLDTELWRVGQELSQQRRKIIPQLAKAAVKQLKDLGFAQCHFDISIT
                     TLDQAAFTQHATRNMPTGLDTIEFQFAPNLGEPPRPLRAIASSGEMARVMLALKTVLA
                     QVDEIPVLVFDEVDANVGGETANAVGEKMRQIARQHQVLCITHLAPVAAQAESHYVVS
                     KQVKEGRTISEITLLNNKERVTELARMLGGQSDAARKHAEELLK"
     gene            984..3083
                     /locus_tag="VG754_04440"
     CDS             984..3083
                     /locus_tag="VG754_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWF18487.1"
                     /translation="MLTALCLAVLTSARAIDETWEYSVQVSATVQSSPAQIHLTWPQD
                     TWLAPSSYTVYRKLLSDTSWGAGTILSGGATDFIDTNVVNGSAYEYQIVKVTSQYTGY
                     GYIYAGINVPLTESRGTMILIVDDNFSAPLTNQLFQLEQDLVGDGWTVLRHDVSTNDS
                     VEYVKSLIQSDYNSDPADVNTVFLFGHIPVPYSGDIAPDEHVPAHQGAWPADVYYGNL
                     FGNWTDYTINDAKATDPRNWNVPGDGKFDQSSIPGQVQLMVGRVDLHNLPGVTYSGNA
                     IFPSEQTLLANYLTKDHNFRQKVFNLPRLGVVGDFFGNYGGEAFAASGWRNFAPFFGA
                     QNVLDLTKDGTWISTLNWDPFLWSYGCGPGDWNTISDLGNDDKYYTAVTPDFAQANIQ
                     TVFTMLFGSYFGDWDVKDDLMRGVLAAPSYTLACLWSGSPHWYCHHMALGQTLGFATR
                     LTQNNGPNGLYKNQVMNYAGLVHIALMGDPTLRMHVVGPPANLSASASAGNVQLIWNG
                     SSDSIAGYNIYRSSSPMGPFTRLNNQLVAGTSYTDTNPSAAAQSYMVRAVKLENTPSG
                     TYYNQSQGIFSSPIWADPFFTGEASLGNDWYYLGSDTNHIFGYYSMQYFPYVFHWDMG
                     WEYYIDAGDAGHGAYFYDFTDDVFFYTAPGVFPYIYDFNVNAWMFYDPLAGQPGRYTS
                     NPRWFYNLSTQAWGNHL"
     gene            3304..>3491
                     /locus_tag="VG754_04445"
     CDS             3304..>3491
                     /locus_tag="VG754_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HWF18488.1"
                     /translation="MNAALPVNESARLEALRLYQILDTKPEQAYDDLTFLASTICETP
                     ISVMALVDEKRQWFKSATG"
BASE COUNT          805 a         1017 c          885 g          784 t
ORIGIN      
        1 cgtttggagg aactcgagga gcggctgaat ttgctccatt cgctcaagcg caagtacggg
       61 gcaaccgtcg cggaagtcat cgcattcggc ggcgaagcga aacgcaagct ggagcaattg
      121 gagcaacgcg acgctgaact cgcgcgtttg aatggcgaat tgaaaaaatt ggacacagaa
      181 ctttggcgag tcggccagga gctttcccaa cagcgtcgca aaatcatccc gcagctcgcc
      241 aaggccgccg tcaagcaact caaagacctc ggcttcgcac aatgccattt tgacatttca
      301 atcacaacgc tcgatcaggc cgccttcacg caacacgcaa cacgcaacat gcccacgggt
      361 ctcgatacca ttgaatttca attcgctccg aaccttggcg aaccaccgcg cccgttgcgc
      421 gccatcgctt cttccggcga aatggcccgc gtgatgctcg ccttgaaaac cgtccttgct
      481 caggtggatg aaattcctgt gctggtcttc gatgaagtgg acgcgaacgt cggcggcgaa
      541 accgcgaatg cagtcggcga aaaaatgcgg caaatcgcgc gtcagcatca ggtcctgtgc
      601 atcacgcacc ttgcgcctgt tgctgcgcaa gccgaatcgc attatgtcgt cagcaaacag
      661 gtcaaggaag gccgcaccat ttccgaaatc acattgttga ataacaagga acgcgtgacc
      721 gaactcgccc ggatgcttgg tggccaatcc gacgccgccc gcaaacacgc cgaggaattg
      781 ctgaaataga ggccaacact ccagtaacta ccttgggcgc accttaaagc aaccccgttt
      841 acaccccatt gaaggttcac tccgcttttg ggcattattg cccgcaacag gaggtaacct
      901 gaaatatgct gagtgctgaa attgcgtgtg aacattcaaa aaagccgtgg caacggcttg
      961 ggaaaatctt tttttggaaa ctattgctga ccgctctctg ccttgcggtt ttaacttccg
     1021 cgcgggcgat agacgagacg tgggaatatt ccgtccaggt cagcgccact gtgcaatcgt
     1081 cccctgcgca aatccacctg acctggcctc aagacacatg gttggcgccc agcagctaca
     1141 cagtttaccg aaaattgctt tccgatacca gttggggcgc gggcacaatc ctgtccggcg
     1201 gcgcgaccga tttcattgac accaatgtgg tcaacggctc cgcttacgaa taccaaatcg
     1261 tcaaggtgac ctcccaatac accggttacg gctacatcta cgccggcatc aatgtcccgc
     1321 tgactgaatc ccgcggcacg atgatcctga tcgtggacga caatttctcc gcgcccttga
     1381 ctaatcagct ttttcaactg gaacaagacc tcgtgggtga cggttggacg gtgttgcgcc
     1441 acgatgtttc aaccaacgat tcggtggagt acgtgaagtc tctcatccaa tctgattaca
     1501 attccgatcc cgccgatgtg aacacggtct ttcttttcgg ccacattcct gttccctatt
     1561 ccggcgacat cgccccggat gagcatgtgc cggcccacca aggcgcatgg ccggcggatg
     1621 tctattacgg aaatttattc ggcaactgga ccgattacac catcaacgac gccaaagcca
     1681 ccgatccccg caactggaac gtccccggcg acggcaagtt cgatcaatcc tccatccccg
     1741 gacaagtcca gctcatggtt ggtcgtgttg atcttcacaa cctgcccggc gtgacttact
     1801 cgggtaacgc catatttccc agcgaacaaa ccctcctcgc caactacctg accaaggacc
     1861 acaacttccg gcagaaagtt ttcaacctgc cgcgcctggg cgtggtaggg gatttctttg
     1921 gcaattatgg cggcgaagct tttgccgcaa gcggctggcg caatttcgcc ccgttcttcg
     1981 gcgctcaaaa tgtcctcgac ctcaccaaag acggcacttg gatatcaacc ctcaattggg
     2041 accctttcct ctggtcctac ggctgcgggc cgggagactg gaatacaatc tccgaccttg
     2101 gcaacgatga taaatattac acagccgtca cgccggactt tgcccaggcg aacatacaaa
     2161 cagtttttac catgttgttc ggcagctatt ttggcgattg ggatgtgaag gacgatttga
     2221 tgcgcggtgt tctggcagcg ccgagctaca cgcttgcctg tctttggagc ggaagtccgc
     2281 actggtattg ccatcacatg gcccttggtc aaacgctcgg cttcgcaacg cgattgaccc
     2341 aaaacaatgg ccccaatggc ctgtacaaaa accaggtcat gaattatgcc gggttggtcc
     2401 atatcgcatt gatgggcgat cccactttgc ggatgcatgt cgtcgggcca cccgccaatc
     2461 tgtcggccag cgcaagcgca ggcaacgttc aactcatttg gaatggctcg tcggattcca
     2521 tcgccggcta taatatttac cggtcatcca gcccaatggg accgttcacc cggctgaaca
     2581 accagttggt ggccggcacg agttataccg atacaaaccc ttccgcagcc gcgcagagct
     2641 acatggtgcg ggccgtcaaa ctggaaaaca cccccagcgg cacttattat aatcagagcc
     2701 agggaatctt ctcctccccg atctgggcgg acccgttttt caccggcgaa gcttcgctgg
     2761 gaaatgattg gtattatctt ggcagcgaca ccaaccacat atttgggtat tacagcatgc
     2821 aatatttccc ttacgtgttt cattgggaca tgggttggga atactacatc gatgccggcg
     2881 atgcaggcca cggcgcgtac ttttatgatt tcaccgatga tgttttcttt tacaccgccc
     2941 ccggcgtgtt tccgtacatc tatgatttca acgtgaacgc atggatgttt tacgatcccc
     3001 tggctgggca acccggccgt tacacctcca atccgcgctg gttctacaat ctttcgacgc
     3061 aggcgtgggg caaccatctt taagcacgaa aagagtgctg aagagttgtt aaagccagcc
     3121 gcagcaagcc agagataccc tgagtgttcc aagctgctgc ggctcgtcct gcggacacag
     3181 ccgcgttccg ttccccattt atgagacttg tagggcgttt tgaagggcgc atttcctgtt
     3241 atcgggtggt tccggtgttt gatggcatcc tacgtgctga tatttgacta agttaagtca
     3301 aatatgaacg cagcattgcc agtcaacgaa tccgcgcgat tggaagcgct caggctgtat
     3361 caaattctgg acaccaagcc ggagcaggcg tacgacgacc tgacgttcct ggcttcgacc
     3421 atttgtgaaa caccgatttc ggtcatggcc cttgtggacg aaaaacgcca atggttcaag
     3481 tccgccacgg g
//
LOCUS       DASZMM010000186         6757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_890739, whole genome shotgun sequence.
ACCESSION   DASZMM010000186 DASZMM010000000
VERSION     DASZMM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890739"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VG754_04450"
     CDS             complement(<1..237)
                     /locus_tag="VG754_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18489.1"
                     /translation="MERKAGELPASAWTQHGEEYHAFFRHQPGRPECAPVICNGAAAG
                     RDGLFDQLGFTACDEGQTNAGRVFDRQCLLLNHIQ"
     gene            complement(298..1935)
                     /locus_tag="VG754_04455"
     CDS             complement(298..1935)
                     /locus_tag="VG754_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWF18490.1"
                     /translation="MILCVSLATGSLLAADIGTPAIIPLPQKMTLHTGTFKLTANTRI
                     SVGTGAHETAKFLLEPLRKSTGYPFKVSDKSASGAAKGGILLMSDDSNTSLGAEGYQL
                     TVAPDSVVIRAPTQAGLFYGVQTLLQLLPPEIFSTNLVTNADWQMPCVQIEDWPRFKW
                     RGLMLDVSRHFYKKSEVEAILDAMALQKLNTFHWHLTDDQGWRIEIKKYPKLTQIGAW
                     RSGVGFDLDPKSTTAYGPDGRYGGFYTQSDIRDVVKYAAARHITIVPEIEMPGHATAA
                     LAAYPQFSCTGGPFTIPLKGGIFNGIYDPANEATFKFLDDVLVELFQLFPGKYVHIGG
                     DEVPKDTWKNSPDCQALMKREGLKNEEELQSWFVRRIEKFVSSHGHQIIGWSEILQGG
                     VAKNAAVMDWIGGAAEAASSGHNVVMSPTDFCYLDQYQSTNSSTEPRAIGGFISLDKT
                     YSFAPISMNLPSQYESQILGAQGNLWTEYIPNLKHAEYMIFPRETALAEICWSSPSGQ
                     SFADFTRRLQVQYQRFDECGINYRRSLMNVSEAASTK"
     gene            complement(1952..3736)
                     /locus_tag="VG754_04460"
     CDS             complement(1952..3736)
                     /locus_tag="VG754_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF18491.1"
                     /translation="MNGNPVATKTGYNRFLLVVAGLGGLLYGVDVGIISGALPYLEAT
                     SGLSANQLSVIVAAVLLGSVISTLFAGVLADWLGRKKLMTLSGILFVASIPVIALAHG
                     YWPLVFGRLLQGISAGLIGVVIPLYLAECLSASNRGKGTGVFQWLLTLGIVAAAVIGF
                     YFSNRVDEVAKLGDADKLFAFKDAAWRGIFWVSLPPGLLFVVGTFMVAESPRWLFRKG
                     KTDAARAALLRSRTDEQAEIELQEMEETTATEKTKSAGSKTRESLLRRKYVIPFLLAC
                     VILACNQLTGINSIIGYNATILIQAGLDDKLAHIGYLILTLVNFLMTIGAVVLVDRKG
                     RKFLLSLGSAGVIISLICAGMIFRQTESQRVDVKSALQTMVTTNQTLSLVVNEITRLA
                     GRNVPSSLVIIYSFGDFQGATTAIRSDDNTAKPIEINRSGCLPQNKVVAFFSNPFASL
                     DEARTAPLKIENALVTPVPSTRNGWLTAGCLFVFMAFFAIGPGVCVWLALSELMPTRI
                     RSNGMSIALLINQAVSTTIAALFLPAVGKFGYSTIFFAFAGCTVIYFITAAFFLPETK
                     GKTLEEIEARFESNEFDRKSLNKIPHES"
     gene            complement(3950..4633)
                     /locus_tag="VG754_04465"
     CDS             complement(3950..4633)
                     /locus_tag="VG754_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF18492.1"
                     /translation="MIKVNDMVGMAETVDATNEMNTTIAIVDDDASVRRSLTGILQRS
                     PGIKCVGNYASAESAIEEVPRLAPQILLMDINLPGMDGVACVRQLARLAPKTQIIMLT
                     VHDDTTAIFNSLAAGASGYLLKPVRATDLIAAVRDVFAGGAPMTSNIARKVVQSFKQT
                     PPAGHVAEELSPRELEVLDCLTKGFVYKEIAEHLEVSYATVRTHIERIYEKLHVRSRS
                     EAVAKYHHL"
     gene            complement(4752..>6757)
                     /locus_tag="VG754_04470"
     CDS             complement(4752..>6757)
                     /locus_tag="VG754_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWF18493.1"
                     /translation="QQIFTTCAAPERKVWVGTETAGVFLFETNKFTPIAGDWGAVIPQ
                     IFSLFQDSRTNLWAGTSGGLFRLEDGRFQRAPGSLRLQTNWISAIFEDREGCLWLGTK
                     FGLFSLRNGEIAAYAPAADIRCIAEDEAGTLWFGTMGSGLLRLPPDRSGTLGRAENFP
                     AIDARALLCDRSGTLWVGGWGSGLFRRDHDTFANYTSVDGLPSDRIAYISSDNSGGLW
                     LLSNNGIIGIAPETLQKYVRGQSLPLLWLHYSSTEGLANRLCSSWGQPVATRASDGCL
                     WFPDMDQICVFDPAKTLSEFIAPNVLVESVLADGKNMPFTPDESLRCASGTRRFEFHY
                     SVIDLLGSQALRFQYKLEGMDRGWVNAGNQRTAYYSQLPPGNYRFRVMVGARDGQWHE
                     AAAALGLQVIPRFWERRWIQILAATLLALGIAGFFVLKDRRELQRRWEWTEMQNAVEK
                     ERMRIARDIHDDLGSSLTHIAQLSELAQADLAQPKQAKPHIDEIFTTARRVARSVDEI
                     VWAIDPKNDSLELSLAYIFKTAQDYLRAGGIKCRLDLPETLPEYSFSSSVRHHLYLAV
                     REALHNLVKHASATEVQLRLNVNVEMGWLMVIIADNGRGFVIPAETSVGEAHPGAGGG
                     HGLGNMIHRLKTVGGRCELESHPGKGTIIRLVLPLKATLEKRA"
BASE COUNT         1539 a         1841 c         1888 g         1489 t
ORIGIN      
        1 ttgaatatgg ttcaataaca ggcactgccg atcaaacacc cgccccgcgt tggtctggcc
       61 ttcatcacac gcagtaaaac ccagctggtc aaacaagccg tcccgcccgg ccgccgcgcc
      121 gttgcatata accggggcgc actccggcct cccgggctga tgccggaaga acgcatggta
      181 ttcctcgccg tgctgcgtcc aggcactggc cggcagttcc ccggctttgc gctccaaggg
      241 ttccgtccac ttgttgtaaa tatcacgaaa ggctctgaat tcattggaag agcgctttta
      301 tttcgtggaa gcagcttcgg acacattcat caaagaacgg cgataattga tgccgcattc
      361 gtcgaatcgc tggtactgga cttgcaagcg gcgcgtgaaa tcagcaaacg attgcccgga
      421 cggagatgac cagcaaatct ccgccagcgc tgtctcgcgt gggaaaatca tatattccgc
      481 atgtttcaag ttggggatat actccgtcca caaattgcct tgggcgccga gaatctgtga
      541 ttcgtattga ctcggcagat tcatggatat cggagcaaag gaataagttt tgtccagcga
      601 aataaatccg ccgatggcgc gcggctcggt ggaagagttg gtggattgat attggtcgag
      661 gtaacaaaaa tccgttggcg acatgacgac gttgtggccg gagcttgcag cttccgccgc
      721 gccgccgatc caatccatga cagcggcgtt tttggcgacg ccgccctgca aaatctcgct
      781 ccagccgatt atctggtgac cgtgtgagct gacgaacttt tcgatgcgcc ggacgaacca
      841 gctttgcaac tcttcctcat ttttcagccc ttcccgtttc atgagcgcct ggcaatccgg
      901 actgtttttc caggtgtcct tcggcacttc atcgccgccg atgtgaacgt atttgcccgg
      961 aaagagttga aatagctcga ccagcacgtc gtccaaaaac ttgaaggtcg cttcattcgc
     1021 cgggtcatag atgccattga aaatgccgcc tttgagcgga atggtgaacg ggccgccggt
     1081 gcaactgaat tgtggatacg ccgcgagcgc cgcggtggcg tgtccgggca tttcgatttc
     1141 cggcacgatg gtgatgtgcc gggctgccgc gtatttcacc acatcgcgga tgtcggactg
     1201 cgtgtaaaat ccgccgtaac gcccatcagg gccgtaagcc gtcgtggatt tgggatccag
     1261 atcaaaaccg acccccgagc gccacgcgcc aatctgtgtg agcttcggat attttttgat
     1321 ttcgattcgc cagccttgat cgtccgtcaa atgccagtgg aacgtgttca atttttgcag
     1381 tgccatcgcg tcgagaatgg cttccacctc ggattttttg taaaaatggc ggctcacgtc
     1441 cagcatcaag ccgcgccatt tgaatcgcgg ccagtcctca atctgaacgc atggcatttg
     1501 ccagtcggcg ttggtcacca ggtttgttga aaaaatctcc gggggcagca attggagcag
     1561 cgtctgcacg ccgtaaaaca atccggcttg tgtcggggct cgaatcacaa ctgaatccgg
     1621 cgcgactgtg agttggtaac cttccgcgcc caaactggtg tttgagtcat cgctcatcaa
     1681 cagaatgccg cctttggctg cgccggaagc ggacttgtcg ctgactttga acggataacc
     1741 ggtggacttg cgaagcggtt cgagcaaaaa ttttgcggtt tcatgcgcac ctgtaccaac
     1801 ggaaatccgg gtgtttgccg tcaatttgaa tgttccggtg tgaagcgtca ttttttgagg
     1861 caatgggatg atggccgggg tgccgatgtc tgcagcaagg agagaaccgg ttgccagcga
     1921 aacacacaga atcattccgg caataaatga tttaactttc atgcgggatt ttgttaaggg
     1981 atttgcggtc aaactcgttg ctctcaaatc gggcctcgat ttcttccagg gttttccctt
     2041 tggtttccgg caaaaagaac gcagccgtga tgaaatagat cactgtgcaa ccggcaaacg
     2101 cgaagaatat cgttgaataa ccgaatttac cgacggcggg gagaaagagc gctgcaatcg
     2161 tggtcgagac cgcctggttg attagcagcg cgatgctcat gccgttggat cggatgcgtg
     2221 tcggcatcaa ttcggacaac gccagccata cgcacacgcc tggcccgatg gcaaaaaagg
     2281 ccatgaacac gaacaggcag cctgccgtga gccagccgtt gcgagtggag gggacaggcg
     2341 tgacgagcgc attttcgatt ttcagcggag ccgtgcgcgc ctcatcaaga ctggcaaaag
     2401 ggttcgagaa aaatgccacg actttgttct gcggcagaca gccgctgcga ttgatttcga
     2461 tcggcttggc ggtgttatcg tcagaacgga tggctgttgt tgctccttga aaatcgccga
     2521 aggaatagat gatgaccaat gaactgggca catttctgcc ggcgagcctg gttatttcat
     2581 tgaccaccaa cgagagcgtc tggttggtgg tgaccattgt ttgcagggcg cttttcacat
     2641 ccacgcgttg cgactccgtc tggcgaaaaa tcatccctgc gcagatgagt gaaatgatga
     2701 cgcccgcgct gcccagcgag agcagaaatt tccggccttt gcgatccaca aggacgaccg
     2761 cgccgatggt catcaggaaa ttcacaaggg tgaggatcag ataaccgatg tgcgccagct
     2821 tgtcgtccag gccggcttgg atgaggatgg tggcgttgta gccgatgatg gagttgatcc
     2881 cggtgagctg gttgcaagca aggatgacgc acgccagcaa aaacggaatg acatatttgc
     2941 gccgcagcag ggattccctg gttttgcttc cagccgactt ggttttctcg gtagcggtgg
     3001 tttcctccat ttcctgcagc tcgatttccg cctgttcatc tgtgcgtgag cgcagcaagg
     3061 cggcgcgggc ggcgtctgtt tttcctttgc gaaacaacca ccgcggcgac tcggcgacca
     3121 tgaaagtgcc gacgacgaat aacagccccg gcggcaatga cacccaaaaa ataccgcgcc
     3181 aggcagcatc cttgaacgca aacagcttgt cggcatcacc caatttcgcc acttcatcaa
     3241 cgcggttgct aaaatagaat ccaatgacgg cggcggcgac aatgcccagg gtcaagagcc
     3301 attggaacac gcccgtaccc ttgccccggt tcgatgcgct gagacattcg gccagataaa
     3361 gtggaatgac cacgccgatc aatccagcgc tgatgccttg caacaggcgt ccaaaaacca
     3421 acggccagta accgtgggcg agcgcaataa cgggaatgct ggcgacaaaa agaatgccgc
     3481 tcaaggtcat caattttttg cgccccaacc agtcggccag cacgccggca aacagggttg
     3541 agatcacgct gccgagcaac accgccgcga cgatgacgga aagctggttg gcgcttaaac
     3601 cggaagtggc ttcgagatat ggcagcgccc cggagatgat gccaacatcc acgccataaa
     3661 gaagtccgcc caatccggct accaccagga gaaaccggtt gtagccagtc ttcgtcgcaa
     3721 caggatttcc attcatctgt tataaaaccg gaggtttttc gccgtcatca gtccaccacg
     3781 tcaaaaacgc gggttggaat ttctttcacg atgattggat gcatagcaca ttgctagttt
     3841 ttagtggaaa gccaccggct tgcaaccccc cgattttagg ggtggctgga tttcggcctg
     3901 ccaaatgatc aggtgatggt aagagatgcg attacaaccc ggcaagattc tacaaatggt
     3961 ggtattttgc gactgcttcg gatcgggaac ggacatggag tttctcataa atgcgctcga
     4021 tgtgagtgcg gaccgtcgca tagctgactt cgagatgttc ggcaatttcc ttgtaaacga
     4081 atcctttggt cagacagtcg agcacctcca attcacgcgg cgaaagttcc tcggccacat
     4141 gcccggcggg aggtgtctgt ttgaatgatt gcacaacctt gcgcgcgata ttgctcgtca
     4201 tgggcgctcc accggcaaaa acgtcacgca cggcggcgat caggtcggtg gcgcgaaccg
     4261 gcttgagcag atagccgctg gcgccggcgg cgagggaatt aaaaatggcc gtagtgtcgt
     4321 cgtgaacggt tagcatgatg atttgcgtct tgggggcgag gcgtgccaac tgtcgaacgc
     4381 aggccactcc atccatgccc ggcaggttga tgtccatcag cagaatttgc ggcgcaaggc
     4441 gggggacttc ctcaatggcg ctttccgcgc tggcataatt accgacgcac ttgattccag
     4501 gcgagcgttg cagaatgccc gtcaggcttc tccgcacgga tgcatcgtca tcaacaatag
     4561 cgattgtcgt gttcatttcg ttcgtggcat ccactgtctc agccatgccg accatatcat
     4621 tgactttgat cattgtcaga cattttaaac cgctcgattt tgccgcatag tcggtaatcc
     4681 ttccgacacg cgatagcagg accgggaatt ctcatgaatg cagaaatatc gaaaagacgg
     4741 ttgattgtta atcaagcacg tttttccagt gtcgctttca aaggcaacac cagccggatg
     4801 atggttccct tgccgggatg gctttccaac tcgcaacggc cgccaacggt tttgagacgg
     4861 tgaatcatat ttcccaatcc gtgacctcca ccggctcctg ggtgcgcctc gccaactgat
     4921 gtttcggctg ggatgacaaa gcctcgcccg ttatcggcga tgatgaccat cagccatccc
     4981 atttcgacgt ttacgtttaa ccgcaattgg acttcggtgg cggaagcatg cttgacgaga
     5041 ttgtggaggg cttcgcgcac cgcgaggtag agatggtgcc ggacggagga ggaaaacgaa
     5101 tattcaggaa gagtttccgg gaggtcgagg cggcatttga tcccgcccgc tcgcaaataa
     5161 tcctgggcag tcttgaagat gtaggcgagg gacaattcca gcgaatcgtt cttgggatca
     5221 atcgcccaaa cgatctcatc cacggagcgg gcaacccggc gcgcggtggt aaagatttcg
     5281 tcgatgtgcg gcttggcttg ctttggctgg gcaaggtccg cttgtgcaag ttcgctcaat
     5341 tgggcaatgt gcgtcaggct ggaaccaaga tcatcatgga tatcacgggc aatgcgcatg
     5401 cgctcctttt cgactgcgtt ttgcatctca gtccactccc accggcgctg gagttcgcgc
     5461 cggtctttga gcacaaagaa tccggcaatg ccaagggcga gcaatgtcgc ggccagtatt
     5521 tggatccatc gtctttccca aaaacgggga atcacctgca atccaagcgc cgccgccgcc
     5581 tcatgccact ggccatcgcg cgcgccgacc atcacgcgga agcgataatt tccgggcggc
     5641 aattggctgt agtatgccgt gcgctggttt cccgcattga cccagccgcg gtccatgcct
     5701 tcgagcttgt attgaaaacg caacgcttgc gacccaagca ggtctatgac cgaataatga
     5761 aactcaaagc gacgcgttcc ggaagcgcaa cgcaaggatt catccggcgt gaatggcatg
     5821 ttttttccgt cagccaggac ggattccacc agaacatttg gcgcgatgaa ctcggacagc
     5881 gtttttgccg ggtcgaacac acaaatctga tccatgtccg ggaaccagag acagccatcg
     5941 gatgcacggg tggcaacagg ttggccccaa ctggaacaaa ggcggttggc caggccttcg
     6001 gttgaagaat aatgcagcca taacagcggc agactttggc cacgcacgta cttttggaga
     6061 gtctccggcg ctatcccaat gattccgtta tttgaaagca gccaaagacc gccgctgtta
     6121 tcgctgctga tgtatgcaat tcggtcggaa ggaagcccgt caacggaagt gtaattcgca
     6181 aaggtgtcat ggtcgcgccg gaacagacca cttccccaac cgccgaccca aagagtccct
     6241 gaccggtcac aaagcagagc gcgcgcgtcg attgccggaa aattctcggc gcggccaagg
     6301 gttccggaac gatccggcgg taaacggagc agaccgctgc ccatggtgcc aaaccagaga
     6361 gtgcccgcct catcctccgc gatgcagcga atatccgccg ccggggcgta agctgcaatc
     6421 tcaccgttgc gtaaagagaa cagtccgaac ttggttccaa gccataggca tccttccctg
     6481 tcttcgaaga tggccgagat ccaattcgtt tgtaatcgca acgatccggg cgcgcgctgg
     6541 aaacgtccgt cctccagcct gaacaacccg cctgacgttc ctgcccagag gtttgttcga
     6601 gagtcttgaa agaggctgaa tatttgggga atcacagcac cccaatcccc ggcgatgggt
     6661 gtgaatttat ttgtctcaaa caaaaagaca ccggcagttt cggtgccgac ccaaaccttc
     6721 cgctctggcg ccgcacaggt ggtaaaaatt tgctggt
//
LOCUS       DASZMM010000187         4698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3090563, whole genome shotgun sequence.
ACCESSION   DASZMM010000187 DASZMM010000000
VERSION     DASZMM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090563"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VG754_04475"
     CDS             complement(<1..1034)
                     /locus_tag="VG754_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18494.1"
                     /translation="MSLEFSPDPKQESEARITALLLGELTGAEAASLLEAIEADPQLA
                     ALYERLKKTVVIVREVSANPVEPVAAQGATAPLKLSEERRQKLLAQFKTVKPKEFVAL
                     LPQSRPRRNFRVLELAAVLALMAILAGLMLPALSKAKSKAVSVSIRSNLKQIQLAKQI
                     WADENNKTANDSPTLKDLEPYMGRGSQVIPPSISGEKYVIGRVGEQASAELDSEKAKK
                     VFGKAPVGLNTLSGGRIFKFFPSTPPEQLARLTADGQVAFVDGNSANPSIKLRAKFAD
                     VTQSAPGKPAEVPPLAVAQNNRQQIYLPPDDSASTATIVPAAPDQKAEDQFGRLRTLS
                     DGLAQQSGISG"
     gene            complement(1031..1576)
                     /locus_tag="VG754_04480"
     CDS             complement(1031..1576)
                     /locus_tag="VG754_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF18495.1"
                     /translation="MTPEKISLPPGPETIEQLFAALESPLLSYACRLAGELTVAEDIV
                     QEAFMKLHEQFEDVREPRRWLYRTVHNAALNHRRRAGKIVSLDLPAHSGATAGDEVPD
                     NQSLPDEEIARWEGIGLVRLSLETLDERSRELVRLKFEENLSYKEISARTGLKVGHVG
                     YLLHHALKGMADELARNGVVP"
     gene            complement(2096..>4698)
                     /locus_tag="VG754_04485"
     CDS             complement(2096..>4698)
                     /locus_tag="VG754_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HWF18496.1"
                     /translation="IFCWNGGVLVGATPDIVYLKDTKGDGKADLRETVFTGFAEGVER
                     TNVQELMNSMVWSLDNRIHGATSGHGGMVKSLRHPELKPINLHGRDFVIEPRTMTMTT
                     EIGGGQHGLSFDDYGRRFTCNNSDHIRYFQYDDHYAARNPYYTMPPALASIAVDGPAA
                     EVYRISPEEAWRVIRTRWRVASLVSGPVEGGGRSSGYFTGATGITIYRGDAFPKEFLD
                     NAFIGECAGNLLSRKLLIPDDVTLKAQRAPDEQNVEFLASTDNWFRPVQFANAPDGTL
                     YLIDMHREIVEHPWSLPDNIKKLIDLNSGSDCGRIFRIVPDGFKQPELPHLDKATTAE
                     LVAALESPNGWTRDTAARLIYERQDKSAAPLLAKLAAESKFPLARIHALHALDGLGEL
                     QKEHVLKAVGDSEPWVRIHAVKLAENFPEAYSSGDALINKLLQMPGDPSNLVRYQLAF
                     TLGELLDGRNIKALAEIARQDAASPLIQAAVLSSLKNGAGDVFAILSADATFAGSKPG
                     QEFLRQLVALVGAKHQPDEVSGVMSFIQKVSDPALSFSLIRGLGDGLKRGDSSLEKVV
                     PASVLESTFAKAVQMTAEATTGEATRTEAIQLLAFSDYKQAGATLTALLKPGTSEDTQ
                     LAAIDALGHFSDAEIPTELTQHWKGFSPRVKSRAVSVLLSRPERALVLLQAVASGIVL
                     PNDLTTPQSRFLRNHENPDVRKLALQVLGTNTAKQRQAVVDEFQPALALKGDAVAGRK
                     IFEQRCVSCHHLDGLGFAVGPDLLSVKSNGKDKILISILDPSREVAPQYIAFQVDTKD
                     GESSVGIIANESTSGITVRQAYGKEDVIPRSNIKGMKSQQQSLMPEGLEQGLSKQDVA
                     NLLEFIIAAK"
BASE COUNT          976 a         1325 c         1298 g         1099 t
ORIGIN      
        1 ccgcttatgc cggattgctg ggccaagccg tcgcttaaag ttctcaacct gccaaattga
       61 tcttcggctt tttgatccgg cgcagcggga acgatagtcg ctgtggacgc actatcgtcg
      121 ggaggcagat aaatttgctg ccgattgttt tgcgcaacgg ctaaaggtgg aacttccgct
      181 ggcttgccgg gagcggactg cgtaacgtcc gcaaacttgg cgcgcaactt tatggaagga
      241 tttgcgctgt tcccgtcaac aaatgccact tggccgtccg ctgtcaaacg cgccagttgt
      301 tctggtggcg tgcttggaaa gaatttgaat atgcgtccgc cgctgagagt attgagtccg
      361 accggagctt ttccaaaaac ttttttggcc ttttcagaat ccaattccgc ggaagcctgt
      421 tcacccaccc ggcctatcac atatttctct cctgaaatcg acggcggtat aacttgcgaa
      481 cctcgtccca tgtaaggctc caaatccttg agcgtaggtg agtcgttggc ggttttgtta
      541 ttttcgtcgg cccatatttg tttcgccaac tgaatctgtt tcaggttgct tcgaatggaa
      601 accgatactg cctttgattt agccttggac aaagcaggca gcatcaggcc ggcgagaatc
      661 gccatgagag ccaaaactgc cgcaagctcc aggacacgga agtttcgacg tggtcgtgac
      721 tgtggcagca gcgcaacaaa ttccttcggc ttcacggtct tgaattgcgc gagcaatttt
      781 tgacggcgct cctcggaaag tttcagcggt gcggttgcgc cttgcgcggc gaccggctca
      841 actgggttgg cggaaacttc gcgcacaatc acgacagtct tcttcaaccg ctcgtaaagt
      901 gcggccaatt gcgggtcggc ttcgatggct tcgagcagcg acgcggcttc cgcgccggtc
      961 aattcgccca gcagcaacgc cgtgatgcgc gcctcagatt cctgtttcgg gtcgggtgaa
     1021 aattccaagc tcatgggaca actccgttcc tggccagttc atcggccatg cctttgagcg
     1081 cgtgatgcaa taaataaccg acatgcccca ccttcaagcc ggtgcgcgcg ctgatttcct
     1141 tgtaagaaag attttcctcg aacttcagcc ggaccagttc gcggctgcgt tcatccaaag
     1201 tctccagact caaccgcacc aggccgatgc cttcccaacg cgcgatctcc tcatcgggca
     1261 gcgattggtt gtcggggact tcatcgccgg cggtcgcgcc ggaatgcgcg ggaaggtcga
     1321 gcgaaacaat cttgccggcg cgtcggcggt gattgagcgc ggcgttatgg accgtgcgat
     1381 aaagccatcg gcgcggttcg cggacatctt caaattgctc gtgcaacttc atgaatgctt
     1441 cctgcacgat gtcttcggcg acggtgagtt cgcctgccag acggcacgcg taactgagca
     1501 gaggcgattc gagcgcggca aaaagctgct caattgtctc cggcccgggc ggcaagctga
     1561 ttttttctgg cgtcatcacg tccatgtgat acgaatctcc aaaatcaggc tcattttcga
     1621 cccgtcccgg cgtatgtttc gcaatgcaac atcgtgtcta tcatccatga cacgcgggcg
     1681 gcgggtttat tagaggaaag tttcgtgaca aaagttaaat ggtgcaaggt tgcatcgtta
     1741 catgggtagt ttgttgcgat gctggtgttt acaaagcatt aactcctggt tggcggcgtt
     1801 tccatccatc cactaaacac cgcaaaagcc tcaacgatgc acctttgtaa ccatgccgtg
     1861 tcaccatatc ccgcttcgat ctcgtaacgc aggcttgtag cctgctgtat cgcagagttg
     1921 cactctgcga aacttattag attaaaatgg tcgccgagat tttatatagc tgcttgcttt
     1981 tcgcgcaact gccgattgca aatcggcgat acagcagatt gaaaatctgc gctacggctt
     2041 cgggctgcaa tcctgcagcg gtttgaaatt taattttgtc ttacaacgaa tccactcact
     2101 tggccgctat gatgaattcc agcaagttgg ccacatcctg cttcgacagg ccctgctcca
     2161 agccttcggg catcaaggat tgttgctggc ttttcatccc tttgatgttc gagcggggaa
     2221 tgacgtcttc ctttccgtaa gcctgccgca cggtgatgcc ggaagtgctc tcgttggcga
     2281 tgatgccgac ggagctttcg ccgtccttgg tatcaacttg aaacgcgatg tattgaggcg
     2341 cgacttcgcg gctcggatcc aaaatgctga tgagaatctt gtccttgccg ttgctcttga
     2401 ccgaaagcaa atccggcccc accgcgaaac caagaccatc gagatgatgg cacgagacgc
     2461 agcgttgctc gaaaatcttt cggcccgcca cggcatcgcc cttcaacgcg agcgcgggtt
     2521 gaaactcatc cacgacggct tggcgttgct ttgcagtgtt tgtgccgagg acttgcaagg
     2581 cgagtttgcg gacatccgga ttttcgtggt ttcgcaaaaa tctgctctgc ggcgtggtca
     2641 aatcgttcgg caaaacaatg cctgatgcca ccgcctgcaa caaaaccaaa gcgcgttcgg
     2701 gccgcgagag caacacggac acagcacgac ttttcacgcg cggcgaaaac cctttccaat
     2761 gctgtgttag ctcggttgga atttccgcgt cggaaaaatg cccgagcgca tcaatcgcgg
     2821 ccaattgcgt atcttcagat gttccgggtt tgagcaaggc tgtgagtgtc gcgcctgctt
     2881 gcttgtaatc ggaaaacgcg aggagttgaa tcgcttcggt gcgcgtggct tcgccggtcg
     2941 ttgcctcggc agtcatttgg acggcctttg caaaagtgct ctccaagacg ctggcgggaa
     3001 ctactttttc gagcgaagaa tcgccgcgtt tcaagccatc acccaaaccg cgaatcaacg
     3061 aaaaagaaag cgcgggatcg ctgaccttct ggatgaatga catgacgccc gagacttcat
     3121 ccggctggtg cttcgctccg acgagcgcga ccagttgacg caaaaattcc tgacccggct
     3181 tggagccggc gaacgttgcg tcagcagaca aaattgcaaa aacatcgccc gctccattct
     3241 tcaacgagct gagcacagcg gcttggatca agggactggc ggcgtcttgt cgcgcgattt
     3301 cggcaagcgc cttgatgttt cgtccgtcga gaagttctcc gagcgtgaag gcgagttgat
     3361 agcgaacgag gttggaagga tcgccgggca tctgcaacag cttgttgata agcgcatcgc
     3421 cggacgagta agcctcggga aaattttcgg cgagtttgac cgcatgaatg cgcacccacg
     3481 gctcggaatc accaaccgct ttcagcacat gttccttctg caattcgcca agtccatcga
     3541 gtgcatggag agcgtgaatt cgtgcgagcg gaaatttcga ttctgcggcg agcttcgcga
     3601 gaagcggtgc tgccgatttg tcctgccgtt cataaatcag ccgcgcggca gtatcgcgcg
     3661 tccaaccgtt gggactttcc aacgcggcga ccagctccgc cgtggttgcc ttgtcgaggt
     3721 gcggcagttc gggttgcttg aaaccgtcgg gaacgatgcg gaaaattcgt ccgcaatcgc
     3781 tgccgctgtt gagatcgatc aatttcttga tgttgtccgg caacgaccat gggtgctcga
     3841 caatttcccg atgcatgtcg atcaggtaaa gcgtgccgtc gggcgcgttg gcgaattgca
     3901 ccgggcgaaa ccaattgtcc gtcgaggcga gaaattccac gttctgttca tcgggcgcgc
     3961 gttgcgcttt caaggtgacg tcgtccggta tcagcaattt tcgcgaaagc agattgcccg
     4021 cgcattcgcc gatgaacgcg ttgtcgagaa actccttcgg aaaagcgtcg ccgcgataga
     4081 tcgtgatgcc ggtcgcgccg gtaaaatatc ccgaagacct gccgccgcct tccaccggtc
     4141 ccgagaccag cgatgccacg cgccagcgcg tccggatgac ccgccacgct tcctccgggc
     4201 ttatgcgata gacttccgcc gccggcccgt ccaccgcgat gctcgccagc gccgggggca
     4261 tcgtgtaata cggatttcgc gccgcgtaat gatcgtcata ttggaaatag cggatgtgat
     4321 cgctgttgtt gcaggtaaaa cgccgcccgt aatcgtcaaa gctcaaccca tgctggccgc
     4381 cgccaatttc ggtggtcatc gtcatcgtgc gcggctcgat gacaaaatcg cgcccgtgaa
     4441 gattgatcgg tttcaattcc ggatggcgca atgatttgac catgccgccg tggccgctcg
     4501 tcgcgccgtg gatgcgattg tccaaactcc aaaccatgct gttcatcaat tcctgcacgt
     4561 tcgttctttc gacgccttcg gcgaacccgg tgaacaccgt ctcgcgcaag tcggccttgc
     4621 cgtcgccctt ggtgtccttc agatagacaa tgtccggcgt cgcgccgacc agaacgccac
     4681 cgttccagca aaagattg
//
LOCUS       DASZMM010000188         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2271460, whole genome shotgun sequence.
ACCESSION   DASZMM010000188 DASZMM010000000
VERSION     DASZMM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271460"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VG754_04490"
     CDS             complement(<1..382)
                     /locus_tag="VG754_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWF18497.1"
                     /translation="MRSADAATSAGARFRAALKAEQPLQVVGVINAYAAILAEKSGFQ
                     ALYLSGSGVAAASYGLPDLGVTTLADVLIDVKRITSATSLPLLVDADSGWDSPETTVR
                     KMIQAGAAGIHIEDQVEAKRCGHRP"
     gene            650..928
                     /gene="xseB"
                     /locus_tag="VG754_04495"
     CDS             650..928
                     /gene="xseB"
                     /locus_tag="VG754_04495"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWF18498.1"
                     /translation="MSKAAKAGEAGKVENLPFEEALKRLESIVETMESQDLPLESLLA
                     RFEEGTQLAKVCQAKLADAEVKIQKLERNSAGEAVLKPLTSDLSEEEQ"
     gene            934..>2019
                     /locus_tag="VG754_04500"
     CDS             934..>2019
                     /locus_tag="VG754_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HWF18499.1"
                     /translation="MSRYLDMVDEPAHVKKLTMPQLNQLADEIRHELITKLSKNGGHL
                     GPNLGVVELTIALHAAFNTPNDKFVWDVSHQSYVHKLLTGRKDRFHTIRTTNGLNGFS
                     LRSESIHDCYGAGHAGTALSAALGMAAARDQLGGNEHVVAIFGDAALTNGISFEALNN
                     ISHTTKRFIGILNDNEWSIAKNVGAIASYLNKLITNPRYNRLQKDFERFIRRMPKGEI
                     AIKLGHKAEEALKGAVNEVTLQQNPAHIESDGRGGFGSSLIFEEMGLRYLGPIDGHDL
                     PLLMNSLEFAKSCDQPVVLHVLTKKGKGYDVAIQQPEKFHGTGPYDVQTGASPSPKPG
                     AAPAWQDVFGQTMVKLCQKDNHVVGITA"
BASE COUNT          495 a          537 c          602 g          385 t
ORIGIN      
        1 tgggccgatg accgcaacgc ttggcttcaa cctggtcttc gatatggatt ccggcagcgc
       61 cggcctgaat cattttgcga acggtggttt cgggagaatc ccagccgctg tcggcatcca
      121 cgagcagggg cagggaagtg gcgctcgtga tgcgtttgac gtcgatgagc acgtccgcaa
      181 gcgttgtgac gcccaaatcg ggcaagccat atgaagctgc cgcaacgccc gagccggaaa
      241 gataaagcgc ttgaaaaccg gatttctccg caaggatagc ggcgtaggca ttgatgacgc
      301 cgacaacttg caacggttgt tcggccttga gcgcggcgcg aaagcgagcg cctgcggaag
      361 tggcggcatc tgctgaacgc actcggaaaa accttttgtc cactaaagac gggtagcgaa
      421 aaacgagtgc gtcagcaagg aaatccggat aagacgacat ggctccaagt tgcgaacaat
      481 tggtttcgtg gtacagttgg gcaattggaa agttgaaggc acgcgaccaa tctgccccgc
      541 acgcgattta ttgatcggaa aactcgcgcg ggataaattg ccggaagctt tcactactgg
      601 ttttactccg tgctagacga aagcgccgtt ctcccttaaa tttcttcgta tgtcgaaggc
      661 agcaaaggcc ggcgaggccg ggaaagtgga gaatttgccg tttgaagaag cattgaaacg
      721 gctggagtcc atcgtggaaa cgatggagtc gcaggacctt cccctggaat cgctgctggc
      781 gcgtttcgag gaagggacgc agttggccaa ggtgtgccag gccaagctgg cggacgcgga
      841 agtaaaaatt cagaagctgg aaaggaacag cgcgggcgaa gcggtcctca agccgttgac
      901 gtccgattta tcagaagaag aacaataaca actatgagtc gctatctgga catggtggac
      961 gaaccggcgc atgtcaaaaa attgacaatg ccccaattga atcaactcgc ggacgaaatc
     1021 cggcacgagt tgatcaccaa actttcaaaa aacggcgggc acctcggccc gaacctcggc
     1081 gtggtggaat tgaccatcgc gctgcacgcc gctttcaaca cgcccaatga caagttcgtc
     1141 tgggacgtga gccaccagag ttatgtccac aagctgctca ccgggcgcaa ggaccgtttc
     1201 cacaccatcc gcaccaccaa cggcctgaac ggtttttcgc tccgctcgga aagcatccat
     1261 gattgttacg gcgcgggaca tgccgggacg gcgttgtccg cggcattggg catggcggcg
     1321 gcgcgcgatc agttgggcgg caacgagcat gtggtcgcga tttttggcga cgcggcgttg
     1381 acgaacggca tttcgttcga ggcgttgaat aatatttcac acacgacgaa gcggttcatc
     1441 ggcatcctga acgacaacga atggagcatc gcaaaaaatg tcggcgcgat cgcgagctat
     1501 ctgaacaagc tcatcacgaa cccgcgatac aatcgtttgc aaaaggattt tgaacggttc
     1561 atacggcgca tgcccaaggg cgagatcgcc atcaagctcg gtcacaaagc cgaggaagcg
     1621 ctcaaaggcg cggtcaacga agtcacgctg caacagaatc ccgcccatat cgaatcggac
     1681 ggccgcggcg gtttcggaag cagtttgatt ttcgaggaga tgggactgcg ttatctcggg
     1741 ccgattgacg ggcatgattt gccgttgctg atgaattccc tggagttcgc caagagttgc
     1801 gatcagccgg ttgtgctgca tgtcctcacc aaaaaaggca agggctacga tgtcgcgatc
     1861 cagcagccgg aaaaattcca cggcacaggg ccgtatgatg ttcaaaccgg cgcttcaccg
     1921 tcgcccaagc ccggtgccgc gcccgcatgg caggacgttt tcggccagac gatggtcaag
     1981 ctttgccaga aagacaacca cgtcgtgggc atcaccgcg
//
LOCUS       DASZMM010000189         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2828989, whole genome shotgun sequence.
ACCESSION   DASZMM010000189 DASZMM010000000
VERSION     DASZMM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828989"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VG754_04505"
     CDS             complement(<1..345)
                     /locus_tag="VG754_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18500.1"
                     /translation="MKTFILRSLAIAAIACLTVFYTSNVTARPSNSATLLVDAYAALD
                     RADHDYKGHRIDAMKQIEEAAKVEGVNLRGDGRGHEKQGVSDEQLRVAEGFLQQAKGE
                     LKGKALKHVNNAL"
     gene            complement(402..614)
                     /locus_tag="VG754_04510"
     CDS             complement(402..614)
                     /locus_tag="VG754_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18501.1"
                     /translation="MTIEAVRDALHANAPFKVFTADGRRIDIPHSDFAMLSRSGRLLH
                     VSLENDQTEAIDILLVTFQSTACTNL"
     gene            complement(681..1334)
                     /locus_tag="VG754_04515"
     CDS             complement(681..1334)
                     /locus_tag="VG754_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016957737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18502.1"
                     /translation="MFAYIGAAIFGGYRDVHTSREVKAAVPLMLSHSLVIGYHGCDQK
                     IVERVVSGKDDLKASQNDWDWLGHGIYFWEDSHGRALRWAKEEARQRNPKIKKPAVLG
                     AVIDSGNCLNLADTDALALVNSAHQAYKELCAKSGVAMLKNHGSELKARFLDCVVVET
                     LHELRRQENKEAFDTVRGFFLEGRELYSGAGFRELYHIQICVRSPKQIIGYFLPRNG"
     gene            complement(1468..2940)
                     /gene="ahcY"
                     /locus_tag="VG754_04520"
     CDS             complement(1468..2940)
                     /gene="ahcY"
                     /locus_tag="VG754_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWF18503.1"
                     /translation="MPTVTLPKTSKNRLTAKNGNGAAKHDYAVRDLSLAEWGRKTIEV
                     SEHEMPGLMAIRKKYGPLKPLKGVRVTGSLHMTIETAVLIETLATLGASVRWASCNIF
                     STQDHAAAAIAKAGIPVFAHKGESLEEYWDFTLAALTHPGNKGPQLIVDDGGDATLLI
                     HKGYELENGNDWVNTPSDNHEVAVIKALLKRVAKERPGFWHEVVKDWKGVSEETTTGV
                     HRLYQMLEQGKLLVPAINVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVAVVC
                     GYGDVGKGSAHSLRGFGARVIVTEIDPINALQAAMEGFEVTTLEDTLGTGDIYVTTTG
                     NCDVITLDHILKMKDQAIVCNIGHFDNEIQVDKLNTLKGAKKINVKPQYDKYELPNGR
                     TIYLLAEGRLVNLGCATGHPSFVMSNSFTNQCLAQLDLWKNRDTNKIGVYRLPKHLDE
                     EVARLHLDRIGVKLTKLTKKQADYLGISPDGPYKPEHYRY"
     gene            complement(2964..>3133)
                     /locus_tag="VG754_04525"
     CDS             complement(2964..>3133)
                     /locus_tag="VG754_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HWF18504.1"
                     /translation="EVFSFKPAAIIKQLNLLRPIYSKTTNYGHFGKVDDLNSITWEKT
                     DKASALKKAVQ"
BASE COUNT          643 a          825 c          867 g          798 t
ORIGIN      
        1 gagggcgttg ttcacgtgct tgagcgcctt gccttttaac tcgcccttgg cctgttgcaa
       61 aaaaccttct gcgacgcgga gttgttcatc agatacaccc tgtttctcat gaccgcgtcc
      121 atcgccacgt agattcacgc cttccacctt tgccgcctcc tcgatctgct tcatcgcgtc
      181 aatgcgatgg cccttgtaat cgtggtccgc ccggtccaat gcggcataag catcaaccaa
      241 taatgtggcc gagttggatg gtcttgccgt cacattggag gtgtaaaaca ccgtcaaaca
      301 ggcaatggct gcgatcgcca acgaacgaag aataaaagtt ttcatggttt tatggttgtc
      361 ccgtgcttac acccaatttg acgaatttgg gcattggatg gttacaagtt cgtgcaagct
      421 gttgattgaa atgtgacgag gagaatatca atcgcttcgg tttgatcgtt ctcgagggaa
      481 acatgcaaca aacgaccgga gcgggacagc atggcgaagt cggaatgcgg aatatcaatc
      541 cggcgtccgt ccgccgtgaa gactttgaaa ggcgcgtttg cgtggagcgc gtcccgaaca
      601 gcttcaattg tcatgaccgc aatttaggct gaagccgctg gaatgccaag ccggaaaagg
      661 gtgcgcttcg gcaaacgcga tcaaccattt cgcggcagaa aatagccgat gatttgtttt
      721 ggagaacgga cgcagatttg aatgtggtac agttcacgga agcccgcgcc ggaatacaat
      781 tcgcgtccct ccagaaaaaa tccgcgcact gtgtcaaatg cctccttgtt ttcctgccgt
      841 cgcaattcgt gaagtgtttc aaccaccaca caatcgagaa aacgggcttt cagttctgaa
      901 ccgtgatttt tgagcatggc gacaccagat ttggcgcaga gttccttgta tgcttgatgt
      961 gcgctattta ccaaagccag cgcatcggtg tcggcaaggt tcaagcagtt tcccgaatca
     1021 atgaccgccc caaggactgc gggttttttg attttcgggt tgcgttgtct ggcttcctcc
     1081 tttgcccaac gcaaagcgcg gccatggctg tcttcccaaa agtaaattcc gtggccaagc
     1141 caatcccagt cgttttgact tgctttgaga tcatccttcc cggaaaccac tcgctccacg
     1201 attttctggt cgcaaccgtg atagccaatg accagcgagt gggaaagcat cagcggaact
     1261 gcggctttaa cttcccttga agtgtgtaca tcccggtagc cgccaaaaat cgcagcgccg
     1321 atatacgcga acacgcaatc ctcctgccag cccggcgaag ctgacgggct tcagatttaa
     1381 tcgttttcga gtcggcgtat ttgttaatgg ccactttagt catctactga cattatgcca
     1441 tggagcttca ttccaaacaa ggtgttttta ataccgataa tgttccggtt tgtaagggcc
     1501 gtcgggcgag atgccgaggt aatcagcttg tttcttggtc agcttggtga gcttgacgcc
     1561 gatgcgatcc aaatgcaggc gggcgacttc ttcgtccaaa tgcttgggca accgataaac
     1621 accgattttg ttggtgtcgc ggtttttcca caggtccaac tgcgcgaggc attggttggt
     1681 gaagctgttg ctcatgacga agctcggatg gccggtggcg caaccgaggt tcaccaaacg
     1741 tccttcggcg agcagataaa tcgtgcggcc attgggcaat tcgtatttgt cgtattgcgg
     1801 cttgacgttg attttcttcg cgcccttgag cgtgttgagc ttgtccacct ggatctcgtt
     1861 gtcgaaatgg ccgatgttgc acacgatggc ctggtccttc atcttcaaga tgtggtccag
     1921 cgtgatgacg tcgcaattgc cggtggtggt cacgtaaatg tcgccggtgc cgagtgtgtc
     1981 ttcgagcgtg gtgacttcga aaccttccat cgccgcttgc aacgcgttga tgggatcaat
     2041 ctcggtcaca atcacgcgcg cgccgaaacc gcgcagtgaa tgcgcgctgc ccttgccgac
     2101 gtcgccgtaa ccgcacacaa ccgcaacctt gccggcgatc atcacgtccg tggcgcgctt
     2161 gatgccgtcc gcgagcgatt cgcggcaacc gtaaagattg tcgaacttcg atttcgtgac
     2221 ggaatcgttc acgttgatgg ccggaacgag caactttccc tgttcgagca tctggtaaag
     2281 gcgatgcacg ccggtggttg tttcttccga aacgcccttc caatctttca cgacttcatg
     2341 ccagaaaccg ggacgttcct tggcgacgcg cttcaagagc gccttgatga cggcaacttc
     2401 gtgattgtcg ctgggcgtgt tgacccaatc attgccgttt tcgagttcgt aacctttatg
     2461 gatgagcaac gtcgcgtcgc cgccgtcatc gacgatgagt tgcgggcctt tgttgccggg
     2521 gtgggtcagc gcggcgaggg tgaaatccca atattcttcg agcgattcgc ccttgtgcgc
     2581 gaaaacagga atgccggctt tcgcgatggc cgcggcggcg tggtcctgcg tcgagaaaat
     2641 attgcagctc gcccagcgca ccgaagcgcc aagtgtcgcc agcgtctcga tgagcacggc
     2701 ggtctcaatc gtcatgtgca acgaaccggt cacacggacg cctttgagcg gcttgagcgg
     2761 gccgtatttt ttgcggatgg ccatgaggcc gggcatttcg tgctcggaaa cctcgatggt
     2821 cttgcgcccc cattcggcga gcgaaaggtc gcgcacggcg taatcgtgtt tggcggcgcc
     2881 gttgccgttc ttggcagtga gccggttctt ggaagttttt ggcaatgtta cagtgggcat
     2941 gattttgttc gttaaattgt ttattactgg accgctttct tgagcgccga cgctttatcg
     3001 gttttttccc aggtgatgct gttgaggtcg tccacttttc cgaaatgacc gtagttcgtc
     3061 gtcttggaat agatgggccg caacagattc agttgcttga tgatggcggc gggcttgaag
     3121 ctgaacactt cct
//
LOCUS       DASZMM010000190         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5015962, whole genome shotgun sequence.
ACCESSION   DASZMM010000190 DASZMM010000000
VERSION     DASZMM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5015962"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VG754_04530"
     CDS             <1..975
                     /locus_tag="VG754_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18505.1"
                     /translation="ERRFHVRLLDAEFVVPNPAPAPPRSTNWQVEKFPITNTNGNVAF
                     TLKGIKIKHRQADMDWGGLPEKIEPEYEVTEEGKPSQNWQVLDMSLFDSSGNFASETS
                     EDERFLCPHEDAWKLRVKVFGSEQTRYASNTTWTLRGLKIPAAGSVTPMSESHELEGI
                     TERTIVFAGPGEYVISNGVPVKAAMLTEKLRESSVKTTYNNNSGGFRRTVMKTFNVKA
                     KTAFLMAEIGDITEDQRLTVRATDEQGRDYYAHDWNLWSDKQQADSKTSNVHYMSCRY
                     NQPEAFLSFDLPPDVKTVDLTFCIHTCAVSEFIFKPPMEISAITNAAK"
     gene            1168..2211
                     /locus_tag="VG754_04535"
     CDS             1168..2211
                     /locus_tag="VG754_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HWF18506.1"
                     /translation="MKLRTFQFELQLVHQWAIAGTVNAKKTRTVTFVELTSPDGVVGI
                     GESAPSDRYGETLATVDEFLKKVDAGKLSFDDVPASMAHLDTISRKDFAGKCPVNIAL
                     MDGAGKKAGKPIYDLLGLGFTENKHVTSYTIGIDTPEIVRLKVREAAQYPVLKLKVGG
                     KQDREIMAALREVAPTKTVRLDGNEGWKTKEEALANIEYFAKDKHIEFVEQPMPAGTP
                     RKDLAWLKERSPLPLYADESFVSAADVQLCAECFHGVNVKLIKTGGISGAFEALKAAK
                     QAGLKRMIGCMIESSVLISAGAQLAELADHLDLDTNLLITNDPYFGPVATNGMMSFAN
                     ATEKSGLRVCPRT"
     gene            2485..>3267
                     /locus_tag="VG754_04540"
     CDS             2485..>3267
                     /locus_tag="VG754_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWF18507.1"
                     /translation="MKRTHHCNELRPPHIGQTVTLSGWVHSRRDLGGLIFIDVRDREG
                     RTQTVFDPSDLSKELFDRAASLRSECVVSVTGKVRQRPAGTNNPKIATGEIEIGATAL
                     EVLNMAEVLPFPVDDPEAAAKVNEELRLQYRYLDLRRPEMARNLRIRSKVATATRVFL
                     DEQGFLEVETPTLFKSTPEGAREFLVPSRREPGKFYALPQSPQQFKQILMVAGVERYF
                     QLARCYRDEDQRADRQLEFTQIDLEMSFIEREDIYALVEGMLK"
BASE COUNT          793 a          876 c          928 g          670 t
ORIGIN      
        1 gaaaggcggt ttcatgtgcg cctgctcgat gcggaattcg ttgtgccaaa tcccgcgcca
       61 gcgccgccac gaagcaccaa ttggcaggtc gaaaaatttc ccatcacaaa caccaatggc
      121 aatgtcgcgt tcacgttgaa agggatcaaa atcaagcatc gccaggccga tatggattgg
      181 ggcgggttgc cggaaaaaat cgagcccgaa tacgaagtga cggaagaggg taagccgtcg
      241 caaaactggc aggtgctcga catgtccttg ttcgacagct ccggcaattt cgcgtccgag
      301 acgtcggaag acgagcgctt tctttgtccg cacgaggacg cgtggaagtt gcgcgtgaaa
      361 gtttttggca gcgaacaaac gcgttacgcg tcgaacacca cttggacgtt gcgcggcctc
      421 aaaattccag ccgccgggag cgtgacgcca atgagcgagt cgcacgaact cgaaggaata
      481 actgaaagaa ccatcgtatt cgccgggccg ggcgaatacg tgatctcgaa tggtgtccca
      541 gtgaaggccg caatgttgac tgaaaagttg agagagagtt cggtgaagac gacttacaat
      601 aataacagcg gcggttttcg gaggactgta atgaagacgt tcaacgtcaa ggcgaagact
      661 gccttcttga tggcggaaat cggcgatatc accgaagacc agcgcctgac cgtccgggca
      721 accgatgagc aaggccgcga ttattacgcg cacgattgga atctatggag cgacaaacag
      781 caggcggaca gcaaaacttc caacgtccat tacatgagct gccggtacaa tcaaccggag
      841 gcgtttcttt cattcgacct gccgcccgat gtcaagacag tggacctgac cttctgcatc
      901 cacacctgcg cagtcagtga attcattttc aagccgccga tggaaatctc ggcaatcacc
      961 aacgcggcga aataagtcaa cgacccacga aagctggaga attcgtggct tggacagaat
     1021 caacagaagg gacagaataa aggtccgaag gcgcgcggac attctgaatt ttttctgtcc
     1081 cttctgtcca ttctgtcaaa aatcttaaag cccatagaaa ataattcctt tcgcaactgc
     1141 aatcggtagg atgtgcgcgt gcttgctatg aaactcagaa cctttcaatt cgaattgcaa
     1201 ctggtccatc aatgggccat cgccgggacc gtcaacgcga aaaaaacccg cacagtaaca
     1261 tttgtcgaac ttacctcgcc cgacggcgtc gtcggcatcg gcgaatcggc gccttcggat
     1321 cgttatggcg aaacattggc gaccgtggat gaatttctca agaaggtgga cgcgggcaaa
     1381 ctttcattcg atgacgtgcc cgccagcatg gcgcatctcg acacgatttc gcgaaaagat
     1441 tttgcgggaa aatgcccggt gaacatcgcg ctcatggacg gtgcgggaaa aaaagcgggc
     1501 aagccaattt acgatttgct cggtttgggt ttcaccgaaa acaaacacgt cacttcctac
     1561 accatcggga ttgacacgcc tgaaatcgtg cgactgaagg tgcgggaagc ggcgcaatat
     1621 ccggtgctca agctcaaggt aggcgggaaa caggaccgcg aaatcatggc tgccttgcgt
     1681 gaggtcgcgc cgacgaagac cgtgcgcctg gatggaaacg agggttggaa gaccaaggaa
     1741 gaggcgctcg cgaacatcga atattttgcg aaggacaagc acatcgaatt tgtggaacaa
     1801 ccgatgcccg ccggaacgcc gcgcaaagac ctcgcatggt tgaaggagcg ttcgccgctg
     1861 ccgttgtatg ccgatgaatc gtttgtttct gccgccgacg tgcagttgtg cgcggaatgt
     1921 tttcatggcg tgaatgtgaa attgatcaag accggcggca tcagcggcgc gttcgaggcg
     1981 ttgaaggcgg cgaagcaagc cggattgaag cgcatgatcg gttgcatgat tgaatcgagc
     2041 gtgttgatca gcgcgggcgc gcaacttgcc gaacttgcgg accatctcga tttggacaca
     2101 aatcttctca tcaccaacga cccgtatttc ggtcccgtgg caacgaacgg gatgatgtcc
     2161 tttgccaatg cgacggaaaa gtccggcttg cgcgtttgtc cgagaacata gcgcggagtt
     2221 gcactctgct gtatcgccga cttgcagtcg gcaagtgctc ggaaaggcaa accgcgcgga
     2281 attttaaaaa cgattgccat ttcgcaggcc ttgcagagta gaactctgcg atacagcaga
     2341 ttaaaaatct gcgctacgcc tcgcggaaga attcgaggtc attccgtgaa atttaccctt
     2401 tgaattatgc ggctttttcg gccaaactat ttcttcgcct cgtcgcgggc ggcatggggt
     2461 cgtggcggcg ggctggattg ataaatgaaa cgcacgcatc attgcaacga acttcgcccg
     2521 ccgcacatcg ggcaaacggt cactttgtcc ggctgggtcc attcccgccg cgatttgggc
     2581 ggtttgattt tcattgatgt gcgcgaccgc gaaggccgca cgcaaaccgt gttcgacccg
     2641 tccgatctgt ccaaggaact tttcgatcgc gccgcctcct tgcgcagcga atgcgtcgtc
     2701 agcgtcaccg gcaaggtgcg tcaacgtcct gccggcacca acaatcccaa gatcgccacc
     2761 ggcgaaatcg aaatcggcgc gaccgcgctt gaggtgttga acatggccga ggtgctgccg
     2821 ttcccggtgg atgatccgga agccgccgcg aaggtgaacg aggaattgcg cctgcaatat
     2881 cgttatctcg atttgcgccg gcccgagatg gcccgcaatc ttcgcatccg cagcaaggtc
     2941 gccacggcca cgcgcgtttt tctggatgaa cagggttttc tcgaagtcga gacacccacg
     3001 ttgttcaagt ccacaccgga gggcgcgcgc gaatttttgg ttccaagccg ccgcgagccg
     3061 ggcaaatttt acgcgctgcc gcaatcgccg cagcagttta agcaaatcct catggtcgcc
     3121 ggcgtggaac gctatttcca gctcgcccgt tgctatcgcg acgaagacca gcgcgccgac
     3181 cgccagcttg agttcacgca gattgatttg gaaatgtcgt tcatcgaacg cgaggacatc
     3241 tacgcgctcg tcgaaggcat gttgaaa
//
LOCUS       DASZMM010000191         4967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3092641, whole genome shotgun sequence.
ACCESSION   DASZMM010000191 DASZMM010000000
VERSION     DASZMM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3092641"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VG754_04545"
     CDS             complement(<1..1524)
                     /locus_tag="VG754_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWF18508.1"
                     /translation="MTVLPVIERELRTQARLGFTYLVRVLGATALICACFWFGIVNGF
                     TPKVGPYLFGCLNTTLVVAIWIFAPLICADCISRERREGTVGLLFLTPLKAREIVLAK
                     GLVHGLRSMTLLLAVLPVMTISFLVGGVTWRQALLSGLVNFSCICWALAAGLLASSIS
                     KSWLRSMLLSFGLAGCFSVALMIFTGWRVIYAVRSDPAFILPFPYYGWRLQNAMNYNY
                     LQAALPVGFFSITDLENFWGIMFGSLTHSTQRAWLREECEVAIISVMVLVVAVLIAAW
                     NLRRRWQEEPPSARRLWMQEKFCTPVVGVGFFHRWLRRKLERNPIGWLEQRKWTGRLV
                     TWGWLAVMISFYSAVLSAPNAWGLLGMVQGLLAWMLVGIMASSASGSFQRERESRVLE
                     LLLVSPMSAGQIISGRLRGLWGQFVPTFALLLFVWWILQEGAWPRRDFNFSMIPYFCG
                     AFLTLPVIGLYYSLLRKHFISSFLWTLWMGVLLPFVLKWVLLQSAGFVQALIANFFPS
                     "
     gene            complement(1521..3224)
                     /locus_tag="VG754_04550"
     CDS             complement(1521..3224)
                     /locus_tag="VG754_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18509.1"
                     /translation="MTFLPIVERELRVAARRHLTYWTRVGSAGTLLIIFGLMFSVAMI
                     SRGMGWSFGQVIFSVLKWVAFALTALSGIFIPSDSLSEEKREGTLGLLFLTDLRGYDV
                     VLGKLISLSLQSFYGLLAAFPILALPLMMGGVTGTEFTRTILVMCNTLFLSLAIGLFV
                     SSMSREVLKAMNAAGALTLLFLIGLPWGDMALAHWDSTKWKCILSMASPGYLFTAASA
                     LRPADYWECLLVQNWIAWSLLAAACVIVPRAWQEKSNEKTGGLAALAKRWRFGGTKAR
                     LAFRRKFIGRAPILWLALRDRWLARFALLVTLLVLCGLGYNMYINWANNPLQSITDFS
                     VLFNFGVALWLASQACRLYVEAVRSGAMELILVSPMPPEQIVRSQWKALVRTFLVPVL
                     LLLALRITSDIETIIQLKKSYASMKMAAKSAGAWTNYDWITRQTIQMIGNLLNFLLWM
                     AALAWFGMWMGLKNRKVPIAILMSILFVCLIPWFFELFVMGWTMALLAHMKLPFWLGM
                     VPSIVFEAAKNIFFILWSRKRLMTRFRETVVRTGQAPRPRLKFLPRPVPVQAATPPLV
                     S"
     gene            complement(3417..4379)
                     /locus_tag="VG754_04555"
     CDS             complement(3417..4379)
                     /locus_tag="VG754_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF18510.1"
                     /translation="MNAAADSAPPVPAVHTIGLTRTYGAMTALSSLDLTVMRGDLFGF
                     IGSNGAGKTTTLRILATFLAPSAGTARVLGHDVVKDADAVRHVIGYMPDFFGVYKDME
                     VTEYLDFFGACYKIPTAQREKTVNDVLELVGLTEKKGALIGALSRGMQQRLGLARVLI
                     HDPQLLLLDEPASGLDQRARIEMMAILQELQRLGKTIIISSHILSELQTLCNRVAIIE
                     KGRLIYSGPVQGVRDQMASGLIVWVKVASESDKAVELLKTRPEVAEVASVDGRIKVLM
                     VDHDTDHSFVADTLVRGGLKLVGLQEDELGLEEVFLRVTRGETQ"
     gene            complement(4389..>4967)
                     /locus_tag="VG754_04560"
     CDS             complement(4389..>4967)
                     /locus_tag="VG754_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18511.1"
                     /translation="NFKADIFVPVWTSQLYASDWLEAAPLPVKLDVTTDGSDWSVSVM
                     NLREHSLTNAHLVLGGRIMDLGELPSRQTKTYKFGKEQGRSLHEFVGSIAAGFQGAAG
                     SRQNAFGASGGGHIGDLPNSTIAASFISNLENERFITPPGLDLSQVMEHGNAILLAWE
                     SDYSPIKPLNQFPTRRGHIDTLWRLAVPVNAQ"
BASE COUNT         1264 a         1517 c         1258 g          928 t
ORIGIN      
        1 agacgggaag aaattggcga tcaatgcctg aacaaagccc gcgctttgca ataacaccca
       61 cttcaacaca aacggcagca gcactcccat ccagagtgtc cagaggaatg acgaaatgaa
      121 atgttttcgc agcaatgaat aataaagccc gatcactggc agcgtcagaa aagccccgca
      181 aaaataggga atcatgctga aattgaagtc ccttctcggc caggcgccct cttgcagaat
      241 ccaccagaca aacaacagaa gcgcgaacgt gggaacgaat tggccccaca atccgcgcaa
      301 tcgtccggaa atgatttgtc ccgcgctcat tggcgacacc agcaacaatt ccagcacacg
      361 gctctcgcgt tcccgttgaa agcttcccga cgcgctgctg gccatgattc ccaccagcat
      421 ccacgcgaga agcccttgca ccatcccgag aagaccccat gcgtttggcg ctgaaagcac
      481 cgcgctgtag aacgatatca tcaccgcaag ccatccccat gtgaccaatc gtcccgtcca
      541 ttttcgttgt tccagccagc cgatgggatt tcgctccagc ttgcgccgca gccaccgatg
      601 aaaaaagccg acacccacga ccggcgtgca aaatttttcc tgcatccaaa gccgccgcgc
      661 ggaaggcggt tcttcctgcc atctgcgccg taagttccac gccgcaatca agacagcgac
      721 aaccagcacc atcacagaaa ttatcgctac ttcacattcc tcccgcaacc aggcccgttg
      781 cgtgctgtgc gtcagggaac caaacatgat tccccaaaaa ttttcgaggt ccgtgatgct
      841 gaaaaatcca acgggtaacg cggcctgaag gtagttgtaa ttcatcgcat tttgcaaccg
      901 ccagccataa taaggaaatg gcaggataaa cgccgggtct gagcggaccg cataaatgac
      961 gcgccagccg gtaaaaatca tcaacgccac agaaaaacat ccagccaatc cgaatgaaag
     1021 caacatcgag cgcaaccatg atttcgaaat tgaagaagcc agcaagcccg ccgccaaggc
     1081 ccagcagatg caactgaaat tcaccagccc ggaaagcagc gcctgcctcc acgtcacgcc
     1141 gcccacaaga aaagaaatcg tcatgaccgg cagcactgcc agcaaaagcg tcatcgagcg
     1201 aagaccatga acgaggccct tcgccaagac gatttcacgc gccttgagcg gcgtgagaaa
     1261 aagcaggccg accgtccctt cgcggcgttc gcggctgatg caatccgcgc agatcaacgg
     1321 cgcaaaaatc cagatggcaa cgacgagcgt ggtgttcagg caaccgaata gatacggccc
     1381 tacctttggc gtgaacccat tcacaatgcc gaaccagaaa caggcgcaaa tcaacgccgt
     1441 cgcgccaagc acacggacaa gataagtgaa tccaagccgc gcctgcgtcc gcaactcacg
     1501 ctcgatgaca ggcaatacgg tcatgaaacc aacggcgggg tcgcagcctg gacgggcact
     1561 gggcgtggaa ggaattttaa ccgaggtcgc ggagcctgac ccgtccggac aactgtctca
     1621 cggaaccggg tcatcaacct tttccgcgac caaagaatga agaaaatgtt cttcgccgcc
     1681 tcgaagacga tggaaggcac catgccaagc caaaaaggca atttcatgtg cgcgagcaaa
     1741 gccatcgtcc agcccatcac gaaaagttcg aaaaaccacg gaatcaggca gacgaaaagg
     1801 atgctcatca aaattgcgat tggcaccttg cggttcttca accccatcca cattccgaac
     1861 catgcgagcg cagccatcca aagcagaaaa tttaataggt tgccaatcat ctggattgtc
     1921 tgccgcgtga tccaatcata attagtccag gcaccggcgc ttttggccgc catcttcatg
     1981 ctcgcatacg acttttttaa ctggatgatc gtttcgatgt cgctggtgat ccggagggcc
     2041 agcagtaaaa gcaccggcac cagaaaagtc cttacgagag ctttccactg gctgcgcaca
     2101 atctgctccg gcggcatcgg tgagaccaaa atcaactcca tcgcgcctga ccgcaccgcc
     2161 tccacgtata aacggcatgc ctgcgaagca agccataaag cgacgccgaa attgaagagc
     2221 accgaaaaat ccgtgatgct ctggagcggg ttattcgccc agttgatata catgttgtat
     2281 ccaagcccgc acaggaccag caacgtcacc aacagggcaa atcgcgccag ccaacgatcc
     2341 cgcaaggcca gccagaggat gggtgcgcgc ccaataaatt ttcgacggaa cgccagccgc
     2401 gccttcgtgc cgccaaaccg ccaacgtttg gcaagggcag ccaacccacc ggttttctcg
     2461 tttgattttt cctgccatgc ccgcggaacg atcacgcacg cggcggccag caagctccac
     2521 gcgatccaat tttgcacaag caggcactcc cagtaatccg ccggacgcaa ggcggaagcg
     2581 gcggtaaaca aataccccgg gctggccata ctaaggatgc atttccattt ggtcgaatcc
     2641 caatgtgcca acgccatatc gccccacggc agtcctatca ggaaaaggag ggtcaacgcc
     2701 ccggcggcgt tcatcgcttt caacacctcc cggctcatgg aactgacgaa caggccgatg
     2761 gcaagcgaca aaaacagggt gttgcacatg acaagaattg tccgtgtgaa ctccgtcccg
     2821 gtgacaccgc ccatcatcag cggcagcgcg aggatcggaa aagcggcgag caatccgtag
     2881 aacgattgca gcgaaagcga aatcaacttg cccaacacga catcataccc gcgtagatcg
     2941 gtcaggaaga gcagcccaag tgtcccttcg cgtttttcct cgctcaacga atcggacggg
     3001 atgaatattc ccgaaagcgc ggtgagcgcg aacgcaaccc atttcagcac ggaaaagatg
     3061 acctgaccga aactccagcc cattccccgg ctgatcatgg caacgctgaa catcaatccg
     3121 aagatgatca gcaacgtgcc ggcagacccc acacgcgtcc agtaggtaag atgccgccgc
     3181 gccgccacgc gcaattcccg ttcgactatc ggcagaaagg tcatgggcgg aggaatatgg
     3241 agtgatggag tattggagtg gtgggcattg aggaactgga tgctggagcg tcgcggtatt
     3301 gcatggtcac ttcaaaatcg tttttttatt tttcctattt ccatcacttc ccttcccaac
     3361 caccccgtcg ttccattact ccatttcctt tcccgccagc actccatcgc agcccctcac
     3421 tgcgtctcac cccgcgtcac gcgcaggaac acttcttcga ggcccagttc atcctcctgc
     3481 aatccgacca gtttcaaacc gccgcgcacc aacgtgtcgg cgacaaagct gtggtcggtg
     3541 tcatggtcca ccatcaatac tttgatgcgg ccatccacgg acgcgacttc ggccacttca
     3601 gggcgcgttt tcaaaagctc gacggccttg tcgctctcac tggccacttt gacccacaca
     3661 atcaatccgc tggccatctg gtcgcgcaca ccctgcaccg gcccgctgta aatcagcctt
     3721 cctttttcaa tgatggcgac ccgattgcaa agcgtctgaa gctcgctcaa aatatgtgaa
     3781 gagatgatga tggtttttcc gagacgttga agctcctgca aaatcgccat catttcgatg
     3841 cgcgcgcgct gatccaagcc actcgccggt tcgtcgagca acagtaattg cgggtcgtgg
     3901 atgagcacgc gggcgagacc aaggcgttgc tgcatgccgc ggctcaacgc gccgatgagc
     3961 gcgccctttt tctccgtcaa gccgaccagt tccagcacgt cattgaccgt tttttcgcgt
     4021 tgcgccgtgg gaatcttgta acaggcgccg aagaaatcga ggtattcggt cacttccatg
     4081 tccttgtaaa cgccgaaaaa atccggcatg taaccgatga cgtgccgcac cgcgtcggcg
     4141 tccttcacga catcatggcc aagcacgcgg gccgtaccgg cagaaggcgc gagaaacgtg
     4201 gcgagaattc gcaacgtggt ggtcttgccc gcgccgttgg aaccgatgaa accaaacaag
     4261 tcgccgcgca tcacggtcaa gtcgagcgaa ctgagcgcgg tcatcgcgcc gtaagtgcgc
     4321 gtaagaccga tggtgtgaac ggccggaacc ggaggggctg aatcagcagc agcattcata
     4381 tttcgaaatc attgtgcgtt gactgggact gcgagccgcc agagtgtgtc tatatggccc
     4441 cggcgtgtgg gaaattggtt cagcggtttg attggcgaat aatccgattc ccacgcgagc
     4501 aggatcgcat tgccgtgctc catcacctgc gataaatcca atcctggcgg cgtgatgaac
     4561 cgttcatttt ccaggttgga aatgaaagat gctgcgatgg tgctgttcgg caaatctccg
     4621 atatggccgc cgccactcgc accgaacgca ttttggcgcg agccagccgc gccttgaaaa
     4681 ccagccgcga tcgaaccaac aaactcatgc aggcttctcc cctgctcttt cccgaacttg
     4741 taagtcttgg tctgtcgcga tggaagttca cccaaatcca ttatccgccc cccgaggacc
     4801 agatgagcgt tggtcaatga atgttcgcgg aggttcatga cggacaccga ccaatccgag
     4861 ccgtcggtgg tcacgtccag cttcacaggc aagggcgcgg cttccagcca atcgctggcg
     4921 taaagctggc tcgtccagac aggaacgaaa atgtccgctt tgaaatt
//
LOCUS       DASZMM010000192         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1991636, whole genome shotgun sequence.
ACCESSION   DASZMM010000192 DASZMM010000000
VERSION     DASZMM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991636"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VG754_04565"
     CDS             complement(<1..411)
                     /locus_tag="VG754_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18512.1"
                     /translation="MALTIHCKCTCASCGTEINYTSDQAGNVVQCPKCREKSRLPEPE
                     RLGLLEHKGPPLPQSRRCEICGSQINFLETACPFCAAERNKRALRNWIILGASAVAVV
                     VIIGAVIHQLNKPKPVVVVPSTPHIMIAAPVARMP"
     gene            complement(541..1227)
                     /locus_tag="VG754_04570"
     CDS             complement(541..1227)
                     /locus_tag="VG754_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWF18513.1"
                     /translation="MYHRRLICFFVVTGVMMAGMAFGQTSTNTFTVNQAIPQGTPNGL
                     ANKQTLDYSGTGLFQITDLQVTLNISGGFNGDYYAYLVHDNGFAVLLNRPGRTSGNSV
                     GYADSGMNITLSGSAATDIHYYQTASNPNGGLLTGLFAEDGRNVDPTTVIGTESRTAL
                     LGSFNGTDPSGEWTLFLADLDYGEQGQLVSWGLVVTAVPEPSSMGLMALGLAGIFGLG
                     IWKRSRKQTV"
     gene            1560..2366
                     /locus_tag="VG754_04575"
     CDS             1560..2366
                     /locus_tag="VG754_04575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HWF18514.1"
                     /translation="MITITDSRVGRTPQANVGQPETAASTASMEVSRANYRVRLAASE
                     QDVRAAQLLRFVVFNLELREGLEHSYATCLDADAFDPICDHLLVEDVRTGEVIGTYRL
                     QTGRKAAQNLGYYSAQEFDFSPYEPLRNELVELGRACVHVNHRSLTVLSLLWKGIANY
                     ITSKGCRYLVGCSSLTSQDPAVGMAAYRELQPCLVREEWRTMPLPPFACDAEPAATAP
                     KIPKLLSAYLALGSKICGPPALDREFKTIDFLTFLDLYSLSPKSVARYLT"
     gene            2622..3425
                     /locus_tag="VG754_04580"
     CDS             2622..3425
                     /locus_tag="VG754_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF18515.1"
                     /translation="MKKPIRIGVRLIRVLALFLFAYLDFNFRLRLFGRASSIRARADW
                     LQTWSRRFLGILRGSVTFRGNPPSRGILVSNHLSYIDVLVLGSLQPLVLVSKSEVRSW
                     PVIGPLTACAGTLYIHRQSRTDVARLNEEMTGVVNSGVIVALFPEGTSSDGSQVLPFR
                     SSLFGPAEEHHWPVTPAWIHYTLKEGSVADEVCYWRDMTFFPHLLNLLSKKSIEAFVS
                     FGTPIEGKMDRKEMARELHSRVCALKEAHLSSYSSVATSRRNPPGIRQA"
     gene            complement(3422..>3713)
                     /locus_tag="VG754_04585"
     CDS             complement(3422..>3713)
                     /locus_tag="VG754_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18516.1"
                     /translation="LHLGSDTSTERVNSAEEFMVSLKKADRLPGWPKNAKGQLVVEKY
                     SNSITFRCWKPGDFSTFYHYEFTRTSKAASWKLQKAWRTNKKNRVIEKYPVP"
BASE COUNT          806 a         1041 c          983 g          883 t
ORIGIN      
        1 gggcatccgc gccacgggcg ccgcgatcat gatgtgcgga gttgaaggca cgacgacaac
       61 cggttttggt ttgttcagtt gatggatgac ggccccaatg atcacgacga ccgcaacggc
      121 tgacgcgccg agaatgatcc agttgcgaag ggcgcgttta ttgcgttccg ctgcgcagaa
      181 ggggcaagcc gtctccaaaa aatttatctg cgacccgcag atttcgcatc tccttgattg
      241 cggcagcggc gggcctttgt gttccaacaa gcccagccgc tccggctctg gcagccggct
      301 tttctcgcgg cacttggggc attggacaac gtttccggcc tggtccgaag tataatttat
      361 ctctgtgccg caactggcgc aggtgcattt gcaatggatg gtcagtgcca tttgtatatt
      421 gtgtttgtct cacgctcctg ttccccattc ccccgtgagt cccgccaaag aaaacacaat
      481 ctgcgcgaaa atggcaactt gaaagattgc agcaagggat ttgaagaaag aagaaacagc
      541 ttaaaccgtc tgcttgcgac tgcgtttcca aatgcccaat ccgaaaatcc cggccaagcc
      601 cagcgccatc aatcccatgc tgcttggttc cggcacagcc gtgacgacca atccccagct
      661 caccaattgc ccttgttcgc cataatccaa atccgccaaa aacaaagtcc actcaccgct
      721 cggatcggtc ccgttaaatg agcccagcaa tgccgtccgg ctttcggtgc ctatcaccgt
      781 cgtcggatcg acattgcgtc catcttcggc gaacaagccc gtcaataaac ctccattggg
      841 attgctcgcg gtctggtaat agtggatgtc tgtcgccgcg gaaccgctca gggtgatgtt
      901 catgccgctg tcggcataac cgacgctgtt gcccgaagtg cgtccgggcc ggttgagcag
      961 cacggcaaat ccgttgtcat gcaccagata agcgtaataa tcgccattga aaccgccgga
     1021 aatgttgagc gtcacctgca agtcggtgat ctggaaaagt ccggtgcccg agtaatccaa
     1081 ggtttgtttg ttcgccaagc catttggagt accctgtggg atggcttgat tgaccgtgaa
     1141 agtgttggtg gaggtttggc caaaagccat gcccgccatc atcaccccgg tcacaacgaa
     1201 gaagcatatc agcctccgat ggtacattgt gtaggttcta tttctgaata ctaagacaca
     1261 gggtacaggt tccatgttcc aaggcaattc atttctcgca aaaagcgaca atttcatcaa
     1321 ggcgaaccct ttttgtaggg tgctgagtaa ctcctttatc cgtaatttta cagaccctta
     1381 accttgctga catttgtttg taactcgatt aaccatcata cgcggtggaa ggctgcgcgc
     1441 ctggagacgg aaacatgcat tttcgagggt tttgcggcgg atttgaaggg aaaatgcgtg
     1501 cggaaacggg ctttacacgc agcccaaaat tgagcaaaaa tcgaaccgag aatctgacca
     1561 tgatcacaat aacagactca cgcgttggca ggacgcctca agcaaatgtc ggccagccgg
     1621 aaacggctgc ttcaacggca tccatggagg tttcgcgcgc caattaccgc gtgcgccttg
     1681 ccgccagcga gcaagacgtt cgcgccgcgc aattgctgcg cttcgtggtg ttcaacctgg
     1741 aattgcgcga aggacttgaa cactcctacg ccacctgcct tgacgccgac gcgttcgatc
     1801 cgatctgtga tcatctgctc gtcgaagacg tgcgcaccgg cgaagtcatc ggcacgtacc
     1861 ggctgcaaac cggtcgcaag gcggcgcaaa acctcggtta ttacagcgcg caggaatttg
     1921 atttttcgcc ttacgaaccg ttgcggaacg aattggtaga gcttggccgc gcctgcgtcc
     1981 acgtcaatca tcgcagcctg accgtcctga gcctgctttg gaaaggcatc gccaattaca
     2041 tcaccagcaa aggttgccgt tatctggtcg gatgcagttc actgacgtcg caagaccctg
     2101 cggtggggat ggcggcttac cgcgagttgc agccgtgtct tgtgcgcgaa gaatggcgga
     2161 cgatgccgtt gccgcctttc gcctgcgacg ccgagcctgc cgccaccgcg cccaagattc
     2221 cgaaactgtt gagcgcgtat ctcgctcttg gctcaaaaat ctgcggcccg ccggcgctcg
     2281 accgcgaatt caagacgatt gatttcctga cgttcctgga tttgtattcg ctgtcgccca
     2341 aatccgtcgc gcggtatctg acttagcaag caaccgcaga tggacacaga tgcacgcaga
     2401 taaaaggtga acttcaaaac atatctcata aatagaaaac ggagcgcggc tgtctccgca
     2461 ggaccagccg cagcagcatg gaacactcaa gatattagcg gcctgctgcg gttggcgctt
     2521 cgcgcacagc cgcgctccgc taatgatgag ctttttttat tgcccatctg cgtttgtctg
     2581 cgttcatctg cggtttaacc taattttcaa ttgctgttgc cgtgaaaaaa ccaatccgga
     2641 ttggcgttcg tctcatccgc gtcctggcgc tttttttatt cgcctacctg gatttcaatt
     2701 ttcgactccg gctctttggc agggcttctt cgattcgcgc gcgcgcggac tggttgcaaa
     2761 cgtggtcgcg acgtttcctg ggaattcttc gcggatcggt cactttccgc ggcaatccgc
     2821 cgagtcgcgg cattctcgtc agcaatcatc tcagctacat tgacgtgctc gtgcttggct
     2881 cgttgcaacc gctcgtgctc gtttccaaga gcgaagtgcg ttcctggccc gtcattggtc
     2941 cgctcacggc ttgcgctgga accctctaca tccaccgcca aagccgcacg gatgtcgcgc
     3001 ggctgaatga agagatgacc ggcgtcgtga attccggggt gatcgttgcg ctgtttcccg
     3061 aagggacaag cagcgacggt tcgcaagtgc tgccgttccg ttcctcgttg ttcggcccag
     3121 cggaagaaca tcattggccg gtgacgcccg catggatcca ttacacgttg aaggaggggt
     3181 cagtcgcgga tgaagtctgt tattggcgcg acatgacatt ttttccgcac ttgttgaact
     3241 tgctttccaa gaaaagtatc gaggcattcg tcagctttgg aactcccatc gaaggcaaaa
     3301 tggatcgaaa ggaaatggcc cgcgagttgc actcgcgggt gtgcgccttg aaggaggctc
     3361 atctttcctc ctacagttcc gtggcgacat cacgccgtaa tccgcccggc atccgacagg
     3421 cttaagggac gggatatttt tcaatcacgc gatttttttt attcgtgcgc cacgcctttt
     3481 gcaacttcca cgaagccgct ttggacgttc tcgtaaattc ataatgataa aaggtggaga
     3541 aatcgcccgg cttccagcaa cggaacgtta ttgaattgga gtatttttca acaacaagtt
     3601 gtcctttggc atttttcggc caaccgggca gccggtccgc tttcttcaag ctcaccatga
     3661 attcctccgc agaattgact cgttcggttg aagtatcgct gccaagatga agc
//
LOCUS       DASZMM010000193         4655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5293782, whole genome shotgun sequence.
ACCESSION   DASZMM010000193 DASZMM010000000
VERSION     DASZMM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5293782"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VG754_04590"
     CDS             <1..704
                     /locus_tag="VG754_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18517.1"
                     /translation="PESMDVKELPPFIFAYFDPQKKSYQTLSHPAVPLVVRPSAASLP
                     PPAFSSANSSDNPPPVMDVSPIKPRIGALAQLSPPLVRQPWFLALQAIPALAWISLLI
                     RRKQAEKLANNPRLRRQRQVEQTIRAGLKDLRQSADAGETEAFFATFFHLLQEQIGER
                     LDLPASAITEAVLDEHLRPLGVPEPRLDELRELFQACNQARYAREATNAELVSLISRL
                     ESTLDELKKMKASSK"
     gene            908..1666
                     /locus_tag="VG754_04595"
     CDS             908..1666
                     /locus_tag="VG754_04595"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18518.1"
                     /translation="MFALIFFLALMTLNLSARDFTADFDSANKFYEEGRYADAVTAYD
                     KLIETGNISEAIYFNRGNAFFKLGQIGKAIASYRQAERLAPRDRELRADLQLARTRAR
                     GGAPYHNDHLRVWLGSLSLNEWTLLTATAFWALFILLALGQWRMELKSRLKNYVQAAA
                     SATVLLGLCFGLALYLDYLTPSAIVVAGEADVRNGPLDESPGVFKVRDGAELEVLDRK
                     DNWLQVQDPAQRIGWLRQDHALIFDSTGIHVPKS"
     gene            1663..2667
                     /locus_tag="VG754_04600"
     CDS             1663..2667
                     /locus_tag="VG754_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HWF18519.1"
                     /translation="MTAISTIPCKQEFFSGKLLMTLALLALASGCATAKSDPKTGVKI
                     IQESNVLRVELNGKLFTEYHYENVPRPYFYPVIGPEGLPMTRKWPLEPSTDEEHDHPH
                     HRSLWFAHGSVNGLDFWTEGKGFCKIVHDGFTEIKSGRESGVIRSRDKWVSTNGAVVC
                     TDDRSFRVYDRSEGVVFDFDVTIHASNGDVTFQDTKEGSMAMRLAETMRLKGKVGHGH
                     IINSEGVRDGQTWGKRADWCDYHGPVDGKIVGVAIFDHPSNPRHPTTWHVRDYGLFAA
                     NPFGLHDFEKKPAHSGDMTIPAGKSVTFRYRFYLHEGDEKEAHVAEMYQEYANAHAAK
                     "
     gene            2722..2940
                     /locus_tag="VG754_04605"
     CDS             2722..2940
                     /locus_tag="VG754_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18520.1"
                     /translation="MRTIEAIIEPGGQVRLIEAVKLSSPRRALVTILEESPSVNDAAL
                     LSEAALAEDWNRPEEDAAWAYLQPAQSS"
     gene            complement(2937..3884)
                     /locus_tag="VG754_04610"
     CDS             complement(2937..3884)
                     /locus_tag="VG754_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF18521.1"
                     /translation="MRKRGRILLSVVILAICGVSLWLTLRPHRDEIVQGKRESERISS
                     FTNGYGSGGSFAQYQREQEEIKEWRALGPQGIEILCRALDKGSSRFTLLYLGYYNGLD
                     DRFQRCLPKPVDTANRRQHAAFLLGSLAPENRDVAVPALIRALKRDPDADVRLNIIGH
                     LDPELLRGKDKERLEILPELIKAAQSYNWGLRHNALTTFQMYPDRLSTVIPILTNALQ
                     DAEGDICWTAVTTLHQLAPDVAKPQIVEAITNMFHEQDEQFFLPGRIEWLGEMRKDAL
                     PAVPALLDALHSTNADVARAASHALVKIAPEMAEKKKTN"
     gene            4255..>4655
                     /locus_tag="VG754_04615"
     CDS             4255..>4655
                     /locus_tag="VG754_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF18522.1"
                     /translation="MANVTELHVNGKDVALDADGERSLLNVLRDDLDLTGSKYGCGEG
                     QCGACTVLLDGVPTRSCITAVGSVGRRKVTTIESLEQDGRLHPLQQAFLDVGAMQCGY
                     CTSGMLMSGFALLQKKPNPSEDEIIRSMNGN"
BASE COUNT         1018 a         1261 c         1298 g         1078 t
ORIGIN      
        1 tgccggaaag catggacgtt aaagaattgc cgccgtttat tttcgcttat ttcgatcccc
       61 aaaagaaatc ataccagacg ctctcgcatc ccgccgtgcc gctcgttgtc cgccccagcg
      121 ccgcatcgct tccgccgccc gcgttttcaa gcgcaaattc cagcgacaat ccgccgccgg
      181 tcatggatgt ctcgcccatc aagccgcgca tcggcgcgct cgcgcaatta tcgccgccgc
      241 tcgtgcggca gccgtggttc ctcgcgttgc aggcaatccc cgcgctggcg tggatttcgt
      301 tgctcatccg gcggaagcag gcggaaaaat tggccaacaa tccccgtctg cggcggcaac
      361 ggcaagtcga gcaaacgatt cgcgccggct tgaaggactt gcgccaatcg gccgatgcgg
      421 gtgaaaccga agcatttttc gccacatttt ttcacctgtt gcaggaacaa atcggcgaac
      481 ggctcgatct gcccgcgtca gcgatcaccg aagcggtgct ggatgaacat ttgcgtccgt
      541 tgggcgtgcc ggaaccgcga ctggatgaat tgcgggagtt gtttcaagcc tgcaatcagg
      601 cccgttatgc gcgcgaagcc accaacgcgg aacttgtctc gctgatttcg aggctggaat
      661 ccacgctcga tgagttgaaa aagatgaaag catcaagcaa atgatttttt caaatgcatt
      721 tttatttggt aattgcccgc cctcatcccg gccttctccc ccggggagaa ggagaagcgg
      781 ttcacacgcg cagtgtttct ggtcgagcgc agtgacaatc cagatgcgag aaaatttaaa
      841 gcagcggaaa acgggaaaac cctctcctgg gggagaggga aggggtgagg gcgaacgttc
      901 cttcaaattg ttcgcattga ttttcttcct cgcgctgatg acgttgaatc tttccgcgcg
      961 cgatttcacg gccgatttcg attcggcgaa taaattttac gaagaagggc gttatgccga
     1021 tgcggtcact gcctacgaca aattgatcga aactggaaat atttcggagg ccatttattt
     1081 caatcgcggc aacgcgtttt ttaaactggg gcaaatcggg aaggccatcg cgtcgtaccg
     1141 ccaggccgaa cgccttgctc cgcgcgaccg cgagttgcgc gcggacctgc aacttgcgcg
     1201 cacacgcgcc cgtggcggcg cgccttatca caacgaccac ttgcgcgtgt ggctcggcag
     1261 cctgagcttg aacgaatgga cgttgctgac ggcgaccgcg ttttgggcgt tgttcatcct
     1321 gctcgctttg ggccaatggc ggatggaatt gaagagcaga ttgaaaaatt atgttcaggc
     1381 cgctgcaagc gccacagtct tgctcgggct ttgcttcggt ctcgcgcttt atctcgatta
     1441 cctcacgccg tcggccatcg tggttgcagg cgaggctgat gtgcgcaacg ggccgctgga
     1501 tgaatcacct ggagttttca aggtgcgcga cggcgccgag ctggaagttt tggaccgcaa
     1561 ggacaactgg ctgcaggtgc aggaccccgc gcaacgcatc ggctggctgc gtcaggacca
     1621 tgcgcttatt tttgattcga cagggatcca cgtgccaaaa tcatgaccgc aatttcaacc
     1681 atcccctgca agcaggaatt cttttccgga aagctgctca tgacgcttgc tttgctcgca
     1741 cttgcgtctg gctgcgccac ggcgaaaagt gatccgaaaa ccggcgtcaa aatcattcag
     1801 gaatccaacg tcctgcgtgt tgagctaaac ggaaaattgt tcaccgaata tcattacgaa
     1861 aatgttccgc gcccgtattt ttatccggtg attggccccg aaggtttgcc gatgacgcgc
     1921 aagtggccat tggaaccttc caccgacgag gagcacgacc atccgcatca tcgttcgctt
     1981 tggttcgcgc atggaagcgt gaatggcctg gatttttgga cggaaggaaa aggattttgc
     2041 aagattgtcc atgacggttt caccgaaatc aaatctggtc gcgagtccgg cgtcatccgc
     2101 tcacgcgata aatgggtttc caccaacggc gcggtggttt gcacggacga ccgctcgttc
     2161 cgggtctatg accgttccga aggcgtcgtg tttgatttcg atgtgacgat ccatgcatct
     2221 aacggcgatg tcaccttcca agacaccaag gaaggcagca tggcgatgcg tttggcggaa
     2281 acgatgcggc tcaagggcaa ggtcggtcac ggccatatca tcaacagcga gggcgtgcgt
     2341 gatggccaga cgtggggcaa gcgcgccgat tggtgcgatt atcacgggcc ggttgatgga
     2401 aagattgtcg gagtcgcgat attcgatcac ccctcaaatc ctcgtcatcc gacaacgtgg
     2461 catgtgcgcg attacggcct gttcgccgcc aatccatttg gcttgcacga tttcgagaaa
     2521 aagcccgccc attccggcga catgactatt cccgcaggca aaagcgtcac cttccgctat
     2581 cgtttctatc tgcacgaagg cgacgagaaa gaagcgcatg ttgcggagat gtatcaggag
     2641 tatgcgaatg cacatgccgc gaaataatta catccatatt gccttgccga tgtcctgcag
     2701 tcggattatc ctcaacatca gatgagaaca atcgaggcga tcattgaacc gggcggtcaa
     2761 gtgcggttga tcgaggctgt aaaattatcg tcgcctcggc gcgctctggt gacaatcctc
     2821 gaggagtctc cttccgtcaa cgatgccgcg ctcttgagcg aagcggcact ggctgaggat
     2881 tggaatcgtc ccgaggagga tgccgcatgg gcttatttgc agccggccca atcctcttaa
     2941 ttggttttct ttttctccgc catctccggc gcaattttca cgagcgcgtg tgaagcagcg
     3001 cgggcaacat cggcattggt gctgtgaagc gcatccagca acgcagggac tgcgggcaaa
     3061 gcatcctttc gcatttcgcc cagccactcg attcttcccg gaagaaaaaa ttgttcgtcc
     3121 tgttcatgga acatgttcgt gatggcttcc acgatttggg gcttggcaac gtcgggagca
     3181 agctgatgca aagtggtcac agccgtccag caaatgtccc cctcggcgtc ctgaagcgca
     3241 ttggtcagaa tgggaatcac cgttgacaaa cgatcgggat acatctgaaa agtggtcaag
     3301 gcattgtgtc gcaatcccca attgtaactt tgtgcggctt tgattaattc cggtaagatt
     3361 tccaatcgtt ccttgtcctt gccgcggagt agctcgggat caagatgccc aataatgtta
     3421 agccgcacat ccgcgtcggg atcccttttc aacgcccgta tcaaggctgg aacggcaacg
     3481 tccctgtttt caggtgccag tgagcctaat agaaatgccg cgtgctgtcg cctgttggca
     3541 gtgtcaaccg gcttgggcaa acaacgttga aaccgatcat ccaacccatt gtaatacccc
     3601 agatacaaaa gcgtgaaccg gctgctcccc ttgtccaaag ccctgcatag gatttcgatg
     3661 ccctgcggtc ccaaagcgcg ccattctttg atttcctctt gttctctttg atattgagca
     3721 aatgatccgc cgctgccata tccattggtg aagctgctga tccgttcact ttcccgtttg
     3781 ccttggacga tttcatcgcg gtgcggacgt aacgtaagcc aaagcgaaac gccgcaaatt
     3841 gcgagaatca caactgaaag cagaatgcgt cctcgtttcc tcatggcgcg atgatgggca
     3901 ggtgaggagg atgacagtca atagtttttt gccgtttccc gagcaggaga gccgacctcc
     3961 ggtccggcgc gttttcaggg agcaatggtc agaatgattt gacaaccatg gtaaactcga
     4021 ctcatggaag ggttgaatat tcaactgcgc caagggcgaa agctacgcct cgaccttcag
     4081 acttccaagt tgaagggtgg acgtggcgca gcctttccag cctgcgggtt caggcgactt
     4141 tccagccgcc tctgtttagt tgcaatttcc gcatttgtgc ggagaaattt ccgcttattt
     4201 ccatcccgtt ccgcttgatt cctgataaaa aaattatttt ttttggtgat ctttatggcc
     4261 aatgttactg aacttcatgt caacggcaag gacgtcgcgc tggacgcgga cggcgagcga
     4321 agcctcctga acgtcttgcg ggacgatttg gatttgaccg gcagtaaata cggttgcggc
     4381 gagggccaat gcggcgcttg cacggtcttg ctggatggcg tgccgacccg ttcctgcatc
     4441 accgcagtcg gttcggtggg gcggcgaaag gtgacgacca ttgaaagcct tgagcaggac
     4501 ggacggttgc atccattgca acaggcgttc ctggatgtcg gcgcgatgca atgcggctat
     4561 tgcacgtcgg gaatgttgat gtcgggcttc gcgcttctgc aaaaaaagcc gaacccgagc
     4621 gaggacgaaa tcattcgctc gatgaacggg aacat
//
LOCUS       DASZMM010000194         5755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_628762, whole genome shotgun sequence.
ACCESSION   DASZMM010000194 DASZMM010000000
VERSION     DASZMM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628762"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VG754_04620"
     CDS             <1..868
                     /locus_tag="VG754_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HWF18523.1"
                     /translation="RLRQTPPYERFFENQNRFLNKRMRRMKANEVFNALVLRRVPDSR
                     ALKKTGGKAFTLIELLVVIGIIGILAGLLLPALARAKEKGRSINCVNNLKQIGVAMQL
                     YVGDFEYYPPGHTAGVTEWDLCVGTYAGGANNALSSAARSKVFVCPSVLVADMGTNLN
                     YSANPNVCKEITASTGQIKADTVPRVSDIIIAADAIQYTVVGNSQAIFWGVQGSSGSF
                     IYWNDGNSANGGSPIQAGSDTDTVLADTDPAGSNFRYRHGNYCAMALFGDGHANRVKK
                     GEVVDQNVYTDY"
     gene            920..3535
                     /locus_tag="VG754_04625"
     CDS             920..3535
                     /locus_tag="VG754_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF18524.1"
                     /translation="MIEKLSESTWDFANSTAPVERSVGVETILKRADDAAGTPKPSSA
                     RQWPKTNGRFLYVGGQRFWIKGVTYGTFRPNADGEPYPSKMQVRDDFAKMREAGINTI
                     RLYTPPPDWLADAAHAEGLFIMADMCWGPRKCELDEPRVSQLHDWIKTHTRRLAEHPA
                     MLLYSIGNEIPPLVVRWYGTKRIEDFLRAQNDIVKEIAPHALTTYVNHPPTEYLTLPF
                     LDVISYNVYLEREPEMRAYLARLQTLTHEKPVMLGEIGLDSLQHGEKTQAQFLDWQIR
                     AAFEKGLCGVMVYSWTDEWGIFNSQIEGWGFGLTNAHRHPKKALDTVRQIYRSDLYRT
                     RKTPWPKVSVVVCCYNAASTLDECLQSLGQLNYPDYEVIVIDDGSKDNTHLIAEKHEM
                     RCIRVPNGGLSKARNLGIEASKGEIVAFIDSDAYADPDWLYYLVSALDEHKASAVGGP
                     NLSPPQDNFIAQCIDQSPGNPTAVLIDNERAEHIPGCNMAYRKDVFDTAGKFDAQHRA
                     AGDDVDLCWRILVTNRKIVYHPSAVVWHHRRPTLRGYLRQQKGYGYAEAHLQRRYPGR
                     FNFFGYPVWEGGVYDSVHSQIRQQGLPIIFRPRIYRGFFCGAQFQTLYQPFLTWWFQI
                     FSTAEWTLLITCTALSGLLAMWSGPKWVSFCLWGLTGIMMLFSIGTCSLAGWRACNVK
                     QWTGWNRWKGLFVVTLLHLIQPLARFAGRVKGRRDLRNDPLDFPATEVLAGDLVKREA
                     WIFHMLNHMKSCGWMVRACSEWDDGDIEILGPGPYKLKLSSVYEEELAHSQFYVRYRV
                     TAKMKIHAPLVVAALMGMLIGITQALYLTPLAVPILFVLVRYVKARTTMIRAVSQMAM
                     ECGWPNGMPKTEAKY"
     gene            3535..5334
                     /locus_tag="VG754_04630"
     CDS             3535..5334
                     /locus_tag="VG754_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF18525.1"
                     /translation="MLHRLFPYWHTAKKETFIGGVILILATFFDLMQPWPIKWLVDYV
                     FGNHAVPTWLTVIWPVFGSKNVAGGITAVCVSILTLALIYRLGNTIGQFFLIRAGAHV
                     VQQLRCHACEHLHGLSLAYHDRKKVGDSLYRVAYDSHAAQTLLNGAIVPTVNGIFVLL
                     GATIIMFRINVLLTLVTMAVTPLFWVIIRGFSRKLDAQSKRYHESESLLVSIIQESLS
                     SIRAVQAFTLEPWTGEKVRGQSKKSLDANQQMTRTQMIYSACAGLAVSLGTVSVVWVA
                     SHQVMNGKLSVGDILVFLAYLGILYQPMNTFSQSAGIIHSASAQLRRVFEIIDTVPDI
                     KDKPDAITLPSVHGRIEFRELSFHYEQESPVLNRVNLTVEPGQVLAVVGRTGAGKTTL
                     ASLLVRFYDPTGGAILLDGHDLRDFKLSWLRQQVSIVLQDPILFSATIAENIAYGKMG
                     ASFEQIQEAARLAQADEFIQALPKGYDTILGERGVNLSGGQRQRLAIARAFLKNAPIL
                     ILDEPTSALDTHTEEALLTCLRKLMRGRTTFVIAHRLSTVRQADLIIVFENGRIIESG
                     RHDELLRRDSVYGGMYRSQWKETDEPETSLAAD"
     gene            5350..>5755
                     /locus_tag="VG754_04635"
     CDS             5350..>5755
                     /locus_tag="VG754_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18526.1"
                     /translation="MRRTIRFQNLIIALILVVMATAFSADGQLIASRMIADLNPGSSG
                     SYPSNLYVYKNSLVFSATTPATGRELWGFDGTNVNLIKDIDPGAGDSTPSDFTTYNGL
                     LYFSAYEPTNGDELYAYDGTNTLRVTDINPGAG"
BASE COUNT         1339 a         1547 c         1508 g         1361 t
ORIGIN      
        1 gcgcttacgc cagacgcccc cttatgaacg gtttttcgaa aaccaaaacc gttttttaaa
       61 caaaagaatg agacgtatga aggctaacga ggtctttaat gctttggttt tacgccgtgt
      121 tccggattcg cgtgctttaa aaaaaacagg gggcaaggct ttcactctta ttgagctgct
      181 tgttgtcatc ggcattattg gaatcctggc tgggctcttg ctgcccgcgc tggccagagc
      241 aaaagaaaag ggacgttcaa tcaactgcgt taataactta aaacagattg gcgtggcaat
      301 gcaactgtat gtgggtgact tcgaatatta tcccccgggg catacggcag gtgtgaccga
      361 atgggattta tgcgttggga cttacgcggg aggcgccaat aatgcgctga gttccgcagc
      421 gcgctcgaaa gtattcgtgt gcccttccgt gcttgtcgcc gacatgggca cgaatctcaa
      481 ctactctgcc aatcccaatg tctgcaagga gataacggct tcaaccggcc agatcaaagc
      541 agacacggtt ccacgtgtga gtgacatcat catcgccgcc gacgccattc agtacactgt
      601 tgtgggaaat tctcaggcga tattttgggg agttcaaggc agcagcgggt catttattta
      661 ttggaacgat ggaaactccg caaacggcgg ctcgccaatc caggcaggct ctgacactga
      721 tacagtgctg gctgacacag atcccgctgg ttccaatttt cgttatcgtc atgggaatta
      781 ttgtgccatg gcgctgtttg gcgatggtca tgcaaatcgt gtcaaaaagg gggaagtagt
      841 ggaccagaat gtttacaccg attattgaga aaactgcccc tcttcgtcct cccgattaaa
      901 atgacaacgg acgcaagtca tgattgaaaa actttccgaa tcaacgtggg attttgcaaa
      961 ttcgaccgcg cccgtcgaaa gaagtgttgg cgtagagacg attcttaaaa gagccgatga
     1021 tgcggccggc acgcccaaac cttcgtccgc ccgccaatgg ccaaagacaa acgggcggtt
     1081 cctttacgta ggtggccaac gattctggat caagggcgtc acttacggaa ctttcaggcc
     1141 aaatgccgac ggcgaaccct acccctcaaa aatgcaggtt cgcgacgact tcgccaaaat
     1201 gcgcgaagcc gggataaaca ccatccgcct ttacacaccg cctcccgact ggctcgccga
     1261 cgccgcccac gcagagggat tgttcatcat ggctgacatg tgctggggac cgcgcaaatg
     1321 tgaattggac gaaccgcgtg tcagccagct tcatgactgg atcaaaaccc acacgcgcag
     1381 gcttgcagag cacccggcga tgctcctgta ttccatcggc aacgaaatcc cgccgttggt
     1441 cgtgcgctgg tatggcacga agcgcatcga ggatttcctt cgcgcgcaaa atgacatcgt
     1501 caaggagatt gcccctcatg cgctcaccac ctacgtcaac catcctccca cagaatatct
     1561 tacattaccg ttcctcgacg ttatttcgta caacgtctat ctggagcgtg agcctgagat
     1621 gcgcgcctat cttgcgcgcc tgcaaacgtt gacgcatgag aaaccagtga tgctcggaga
     1681 gatcggcctt gattccctcc agcacggcga aaaaacgcag gcgcagtttc ttgactggca
     1741 gattcgcgct gcatttgaaa agggattgtg cggtgtcatg gtgtatagct ggacggatga
     1801 gtggggcatt ttcaattcac aaatcgaggg ttgggggttc ggccttacaa acgcgcatcg
     1861 gcacccgaag aaggcactcg atacggtcag gcaaatttac aggagcgatc tctatcggac
     1921 ccgcaaaacg ccatggccaa aagtttccgt ggttgtctgc tgttataatg ccgcttccac
     1981 gctcgacgag tgcctgcaaa gcctgggcca gttgaattat cctgactatg aggtcattgt
     2041 cattgacgat gggtcgaagg acaacacgca cttgattgcc gaaaagcatg aaatgcgttg
     2101 catccgggtt ccaaacggcg gcctcagcaa agcgcgcaat ctcggcatcg aagcctcaaa
     2161 aggtgaaatc gtggcattca ttgacagcga cgcctacgct gaccctgact ggctttatta
     2221 tctggtttcg gcccttgatg aacataaggc ctcagccgtt ggcgggccga atctttcacc
     2281 gccacaagat aacttcattg cgcagtgcat agatcaatcc cccggcaatc ccacggctgt
     2341 gctgattgac aatgagcgcg ccgaacatat tccgggctgc aacatggctt accggaagga
     2401 tgtgtttgat acggcgggca aatttgatgc gcaacatcgc gccgccgggg atgacgttga
     2461 cctatgctgg cgtattctcg tcaccaacag aaaaattgtt tatcatccca gcgccgttgt
     2521 ctggcaccac cgccggccaa cgctccgagg ctatttgcgg cagcaaaaag gttatggcta
     2581 cgccgaagca catctgcaac gccgttatcc cggtcgtttc aacttctttg gatacccggt
     2641 ctgggaaggc ggcgtgtatg acagcgtcca ttcacagatc cggcaacaag ggttgccgat
     2701 cattttccgc ccgcggatct atcgtggctt tttttgcggc gcgcagttcc aaacacttta
     2761 tcaaccattc ctgacgtggt ggtttcagat tttttccact gccgaatgga cactccttat
     2821 cacctgcacg gcactttcgg gcttgcttgc gatgtggtct ggacccaaat gggtttcctt
     2881 ctgcctttgg ggactgaccg ggatcatgat gcttttctcc atcggcacct gctcgcttgc
     2941 gggctggcgc gcgtgcaacg tcaaacaatg gacaggctgg aatcgttgga agggactatt
     3001 cgttgtgacc ctgctgcacc tgatccagcc tctcgcccgt tttgcgggac gcgtcaaggg
     3061 cagacgggat ttgcgaaatg atccgctgga ttttccggcg actgaagtgc tggcgggcga
     3121 tctggtcaaa cgcgaggcct ggatatttca catgctcaat cacatgaaat cctgcggctg
     3181 gatggttcgc gcctgcagcg aatgggatga cggggacatc gaaatcctcg ggcctgggcc
     3241 ttacaaattg aaactatcct cagtttacga ggaagaactg gcacactcac aattttatgt
     3301 ccgataccgc gtgacagcaa agatgaagat ccacgcgcct cttgtggtgg ctgcattgat
     3361 gggtatgctg attggaatca cccaggcgct gtatcttacg ccactggctg tacccattct
     3421 ctttgttctt gttcgctacg taaaagcgcg aaccacgatg atccgcgctg tttcacaaat
     3481 ggccatggaa tgcggctggc ccaacggcat gcccaagacg gaggcaaaat attgatgctg
     3541 caccggcttt ttccatactg gcacactgcc aaaaaagaaa ccttcattgg cggcgttatt
     3601 ctcatcctgg ccaccttttt tgatttaatg cagccctggc cgatcaaatg gctggtggat
     3661 tatgttttcg gcaatcacgc agtgccaacc tggctgactg tcatttggcc ggtattcgga
     3721 agcaaaaatg tggccggggg cattacagcc gtttgtgttt caattttgac cttggccttg
     3781 atttatcggt tagggaatac cattgggcag ttttttctga ttcgcgccgg cgcccatgtc
     3841 gtacagcaac tgcgttgtca cgcgtgcgag cacctgcacg gtctctcgct cgcgtatcat
     3901 gaccgcaaaa aggtgggaga ttcgctctac cgcgtggcct acgattcaca cgccgcgcaa
     3961 acccttctaa acggagccat cgtcccgacg gtcaatggca tttttgtttt gctcggcgca
     4021 acgatcatca tgtttcgcat caatgtgctt ctgacgctcg tcacgatggc agtcacacca
     4081 ttgttctggg tgatcattcg cggattcagc cgcaaactgg acgcgcaatc caaacgctat
     4141 cacgaaagcg agagcctcct ggtttccatc attcaagaat cattgtcctc aatccgggcg
     4201 gtccaggcat ttacgcttga accctggacg ggagaaaaag tccggggcca atctaaaaaa
     4261 agcctcgacg ccaatcagca aatgacccgc acccagatga tttattcggc gtgcgcgggg
     4321 ctggcggtgt ctttgggcac ggtttccgtt gtttgggtgg caagccatca ggtgatgaac
     4381 gggaagcttt ccgtcggcga tattttggtt tttctggctt acctcggaat tctttaccag
     4441 ccgatgaaca cgttcagtca gagcgccgga attatccatt cggcgagcgc gcagctccgg
     4501 cgcgtgtttg aaatcattga tactgtgccg gacatcaagg acaagcccga tgctatcaca
     4561 cttccatctg tccatggccg tattgagttt cgagagttgt cctttcatta cgagcaagaa
     4621 tcccccgtat tgaaccgcgt taatctgaca gttgaacccg gacaggtttt ggcggttgta
     4681 ggccgcaccg gagcaggcaa gacaacgctg gcgagcctgc ttgtccgatt ttatgatcca
     4741 accggaggcg caatcctgct ggatggacac gacttgcgcg atttcaaatt gtcatggctg
     4801 cggcagcagg tcagcatcgt gctgcaagac ccgattttgt tttcagcgac catcgccgaa
     4861 aacattgcct acgggaagat gggtgcttcg tttgaacaaa tccaagaagc ggcaaggctt
     4921 gcgcaggcgg atgaattcat tcaagcattg ccaaaaggct acgacactat tctcggtgaa
     4981 cgcggggtaa atttgtccgg cggacaacgc cagcgtcttg caatcgcgcg tgctttcctg
     5041 aaaaacgccc ccatcctgat tctcgatgag ccgacttccg cgctcgacac acacaccgaa
     5101 gaagcattgc tgacctgcct gcggaagctt atgcgcggtc gaacgacatt tgtcatagct
     5161 catcgccttt ccaccgtgcg gcaggccgat ttaatcatcg ttttcgagaa tggaagaatc
     5221 atcgaatccg gccgtcacga cgaattgctg cgccgggatt cagtgtacgg aggcatgtat
     5281 cgctctcaat ggaaagaaac ggacgaaccc gaaacatcac tcgcggcgga ttagctgcaa
     5341 aatggataaa tgagaagaac gattcgattt caaaacttga tcatcgccct gattttggtg
     5401 gtgatggcca ccgcgttttc ggcagacggt cagcttattg ccagccgaat gatcgccgac
     5461 ctgaatcctg gttccagtgg ctcatacccg tccaacttgt acgtttacaa aaactctctg
     5521 gtcttcagcg ccactacgcc tgccacagga agagaactgt ggggattcga cggcacaaac
     5581 gttaatttga taaaggacat cgatcccggc gccggcgatt ccacccccag cgatttcact
     5641 acatacaacg gattgctcta tttttccgct tatgaaccca ccaacggcga tgagctttac
     5701 gcgtatgacg gcaccaacac cttgcgcgtg acagacataa atcccggtgc gggca
//
LOCUS       DASZMM010000195         4141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3378720, whole genome shotgun sequence.
ACCESSION   DASZMM010000195 DASZMM010000000
VERSION     DASZMM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378720"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="VG754_04640"
     CDS             complement(<1..1575)
                     /locus_tag="VG754_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18527.1"
                     /translation="MRSTATALLATALCAASAARAGFTPIPLSSGSFNQSMIVPASAP
                     APVIAGGYTTASMDNGTANTATSWYEQGYDTSAPTTGLPHPGTTFTSQTVANHSYVMA
                     PSYTSNNAVLLDSTLTSATLVLTSPAAFSKLSLLESGGNHGVMFSYTVHHQSGANDSG
                     SASIQDWFNGSNVSWTANGRVDVQTFTFTSVNGNDPQLYSLDLALINSNSPVTSITFS
                     YVSGGGHGAIMAVSGLNGASCTPLQVTGYNEDIVVEASAGKPGALNVTTATMDAGTAN
                     TANTWYELGYNPTVPTTGLPHPGTTLTNASASDHLYVLPPSYTTNDVALICSNIPTVS
                     ITPATTPPAYQVISFLLASGNGPVTLGYTVHHASGASQVGGLVASDWVDYSPVAYDAN
                     GRVSVNTSTVSYVNSGNPRLYAQDITLGFSNSPVTSVTLTFPTNGANANAVILALSGG
                     SPTFSLVGNDFNANTATAAQVLQQWYNGGGLYNTTGWWNAANCLEAIENDIYANNDVQ
                     YLSVLTNTYEQNGDFLN"
     gene            1751..1993
                     /locus_tag="VG754_04645"
     CDS             1751..1993
                     /locus_tag="VG754_04645"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HWF18528.1"
                     /translation="MPLLKLNRINKGGEIFINSDQIRFIEIETGSSTVNMGPGNVYAV
                     QETPEVIADKIEELHTKRIRDAIVSSGLVDKKTEII"
     gene            complement(2053..2937)
                     /locus_tag="VG754_04650"
     CDS             complement(2053..2937)
                     /locus_tag="VG754_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWF18529.1"
                     /translation="MIFNPTARGEKARRFRRHLDEIGADCALKQTVAAGGARPLAAEA
                     VREGFETVVAAGGDGTLNEVLNGIGDVPDGFERARLGVLPLGTVNVFAKELGMPAGLK
                     QGWQVLREGNETRIDLPMVECAGADGKAYRRYFAQLAGTGLDARAIELADWQLKKKMG
                     PLAYVWAGVEAMRDKPSCVTATNGSHTATGELVLIGNGCFYGGRYRLHPGADLRDGLL
                     EVRVFPKANWLTLLRCAGMFVARMNLPEGVAKSFQSKSLTLTSELPTPIEVDGENIGH
                     LPATFSIQQKKLRVVAPL"
     gene            3156..4046
                     /gene="grpE"
                     /locus_tag="VG754_04655"
     CDS             3156..4046
                     /gene="grpE"
                     /locus_tag="VG754_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWF18530.1"
                     /translation="MSNQTDPKFVKLPFFVCAFFCDAVLIGFAYVYSHHAHSPMGIFE
                     ALLFLLCGAIGAGVFILPFWLEYRAAVRMVETGAVVSTISQIQNIEELAKHIGGATSQ
                     WQGVHEHSVKTVTTAKEIADRITAEAAAFKDFLKKANDAERANLRIEIEKLRRAEGDW
                     LQIIVRMLDHTYALNQAAARSGQPGLIEQLARFQDACRDVVRRVGLVPFVAAANERFD
                     PKVHESHESQALSVPDAKVRETIATGYTYQGQLLRPALVSLENPAPVSAVVKTTEPAS
                     LVEANGDAKEAEISEEPTLL"
BASE COUNT          890 a         1216 c         1121 g          914 t
ORIGIN      
        1 attgaggaaa tccccattct gctcgtacgt gttcgtcagc acggagagat attgcacgtc
       61 gttgttggca taaatatcgt tctcaatcgc ttcgagacaa ttcgccgcgt tccaccaacc
      121 tgtggtgttg tacaaaccgc cgccgttata ccattgctga aggacttggg ccgccgtcgc
      181 ggtgtttgcg ttgaaatcat ttcccacgag actgaacgtg ggcgagccgc cgcttaatgc
      241 caggatgact gcattcgcgt tcgcgccatt cgtcggaaaa gtcagggtga cactagtgac
      301 cgggctgttg ctgaatccga gcgtgatgtc ctgcgcgtaa agacgcggat tgccgctgtt
      361 gacgtaactg acggtgctgg tattcacgct gacgcggcca ttggcgtcat aagcgacggg
      421 cgaataatca acccaatccg atgccacaag cccgccgact tgggatgctc cactggcatg
      481 atgaacggta tatccaagag tcaccgggcc gtttcccgac gcaagcagga acgatatgac
      541 ctgataagcc ggaggcgtgg tcgcaggcgt gatggaaacg gtgggaatgt tggaacaaat
      601 caacgcgacg tcattggtcg tgtaacttgg gggcaaaacg taaagatggt ccgacgcgct
      661 ggcgtttgtc agcgttgtgc cgggatgagg caaaccggtg gtcggcaccg tgggattata
      721 accaagttcg taccaggtat tggcggtgtt ggctgttccg gcgtccatcg tggcggtggt
      781 gacgttgagc gcgcccggct ttccggcgct tgcttcgacg acaatatctt cgttgtagcc
      841 ggtcacctgc aatggcgtgc aactggcgcc attaagtccg ctcaccgcca tgatcgcgcc
      901 gtgtccaccg cccgaaacgt aggaaaacgt gatgcttgtg acaggactgt tggaattgat
      961 caatgcaagg tccaacgaat aaagctgcgg atcgtttcca ttgacggaag taaacgtgaa
     1021 cgtctggacg tccacccgtc cattcgcagt ccaggacacg ttgctgccgt taaaccaatc
     1081 ctgaatgctt gcgctgccgg aatcgttcgc accgctttgg tgatgcacgg tgtaagaaaa
     1141 catcacgccg tggttcccgc cgctttccag gagcgaaagc ttggagaatg ccgcgggcga
     1201 agtcagcacg agtgtcgcgg aggtgagcgt tgaatcgagc agcactgcgt tattggaagt
     1261 gtaactcggc gccatgacgt atgaatgatt cgccaccgtt tggcttgtga acgtcgtgcc
     1321 gggatgcggc agcccggtcg tcggcgcgct ggtatcatag ccttgctcat accaactggt
     1381 cgctgtgttc gcggtgccgt tgtccatcga ggccgtggtg tagccgcccg cgatgaccgg
     1441 ggcaggggcc gaagcgggaa caatcattga ctggttgaaa ctgccgctgc tcagcgggat
     1501 gggtgtgaag cccgcccgcg ctgcgctggc cgcgcacaat gcagtcgcca aaagggccgt
     1561 cgccgtacta cgcatgaagt gtcgtatagt aatatgattt gctgccaaat accagcctaa
     1621 tcagggaggc ttcatcaaag agggcgcaac aagccctcga tatgcgccct ctgccgtcga
     1681 acgcctattt ttcagttgcg cgcgacggca tcggacgtta aaccaaaccg gtcaatcgaa
     1741 cttcacgctt atgccattgc tcaaactcaa ccgcatcaac aagggcgggg aaattttcat
     1801 caacagcgac cagatacgct tcatcgaaat cgaaaccggt tcctccaccg tcaacatggg
     1861 ccccggcaat gtttatgcgg tgcaggagac tccggaagtc atcgccgaca aaatcgagga
     1921 attgcacaca aaacgcatcc gcgacgccat cgtctccagc ggtctcgtgg acaagaaaac
     1981 tgagatcatt tgagcaaggt ctccaacccc ctgtcgcgtc agttctgcaa tttgaaattt
     2041 caaatttgaa attcacagag gcgcgacgac ccgcaatttt ttctgctgaa tggaaaacgt
     2101 cgccggcaga tgcccgatgt tttccccgtc cacttcaatc ggcgtgggaa gttcgctggt
     2161 gagcgtgagg ctttttgatt ggaaactttt cgccactcct tcaggcaaat tcatccgcgc
     2221 gacaaacatc cccgcgcaac gcagcagcgt cagccagttc gccttcggaa acacccgcac
     2281 ctccagcaaa ccgtcgcgca agtccgcgcc gggatgcagc cgataacgcc cgccgtaaaa
     2341 acagccgttg ccgatcagca ccagttcgcc cgtcgccgtg tgcgagccgt tcgtcgccgt
     2401 gacgcatgaa ggcttgtcac gcattgcctc gacgccagcc caaacatacg cgagcggtcc
     2461 catttttttc ttcaactgcc agtcggcaag ttcaatcgcg cgcgcgtcca aacccgtccc
     2521 ggcaagttgc gcaaaatatc ttcgatatgc tttgccatca gcgcccgcgc attcgaccat
     2581 cggcagatca atgcgcgttt cgttgccctc gcgcaacacc tgccagcctt gtttcaaacc
     2641 cgctggcatc cccaattctt tcgcgaacac attgaccgtt cccaacggca gcacgcccaa
     2701 tcgcgcccgc tcaaaaccat ccggcacatc accgatgcca ttgagcacct cgttcagcgt
     2761 gccgtccccg cctgcggcaa ccaccgtctc aaacccttcg cgcaccgctt ctgccgcaag
     2821 cggacgcgcg ccacccgccg cgacggtttg cttcaaggcg cagtccgcgc cgatttcgtc
     2881 cagatgcctg cgaaaccgcc gcgccttctc gccccgcgcg gttggattga aaatcacaca
     2941 aatgcgcaca gattcaatct tgtgggtctg ccgatgagag tcgagtaaaa agctccaaca
     3001 tccaagcgcc aaacttcaac gaataatcaa cttccaatct tcaaaaccgc gcttttgttg
     3061 tgtgaggctt ggtgcttctc tggagcttgg aggttgatgg ttggagcttt catacgcctt
     3121 gaactctctc cgcccttttc ctaaactcgg ccatcatgag taatcagact gacccgaaat
     3181 tcgtgaagct gccgtttttt gtgtgcgctt ttttctgcga tgccgtgctg atcggtttcg
     3241 cttatgtgta ttcgcaccac gcgcattcgc cgatgggcat tttcgaggca ttgctttttt
     3301 tgttgtgcgg cgcgatcggc gcgggagttt ttattttgcc tttctggctc gaatatcgcg
     3361 cggcggtcag gatggtcgaa accggcgcgg tcgtctcgac gatttcacag atccaaaaca
     3421 ttgaggaact tgccaagcat atcggcggcg cgacctcgca atggcagggc gtgcatgaac
     3481 attcggtcaa gactgtcacg accgcgaaag aaatcgccga ccgcatcacc gccgaagccg
     3541 ccgcattcaa agatttttta aaaaaagcca atgacgccga gcgcgccaac ttgcgcatcg
     3601 aaatcgagaa acttcgccgc gccgagggcg attggctaca gatcatcgtg cggatgctgg
     3661 accatactta cgcgttgaac caggctgccg cgcgttccgg gcaacccgga ttgatcgagc
     3721 aactggcccg ctttcaggac gcctgtcgcg atgtcgtgcg gcgcgtcggt ctcgtgccat
     3781 tcgttgctgc cgccaacgaa cggtttgatc cgaaagtcca cgaatcgcac gaatcgcagg
     3841 cgctgtccgt gcccgacgcc aaagtgcgcg agacgattgc caccggttac acctatcagg
     3901 gacaattgct ccgcccggcg ctggtttccc ttgaaaatcc cgctcccgtt tccgccgtcg
     3961 ttaaaactac agagcccgcc tctctggtcg aagcgaacgg cgacgccaag gaagccgaga
     4021 tcagtgaaga gccgacgttg ctttgatcgc tactgcacaa gcaggcgttc caggcttttt
     4081 acgattccgt tcttattttt ttccaatatc atccattttt ggaatgattc gaccattgcg
     4141 c
//
LOCUS       DASZMM010000196         8243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4464879, whole genome shotgun sequence.
ACCESSION   DASZMM010000196 DASZMM010000000
VERSION     DASZMM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4464879"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..1085
                     /locus_tag="VG754_04660"
     CDS             327..1085
                     /locus_tag="VG754_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HWF18531.1"
                     /translation="MNFDISHLLDQWDYQPGTASVRKFTGKDGVEKLQVRLDLGLLQM
                     NCEGRPDGKRPLGHPSLYEYYQARLGKHKTANDGSEEGFLLKAEDCAKLQFEALQYYQ
                     RHNCLWQLEDYAGVARDTERNLLVCDFAAKHAESDELSWSLEQFKPQLLMMHTRARAT
                     ASLKENNHETAVRQIDEGMARIREFYREHERLEPDQSVELNSLKAWLDEINSRRPLSK
                     REKLERELSEAVTNEDYEKAAQMRDALRNLKPTE"
     gene            1088..1534
                     /locus_tag="VG754_04665"
     CDS             1088..1534
                     /locus_tag="VG754_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWF18532.1"
                     /translation="MSLQERISQEIKTAMLAKDADRLSALRMLKSTMGYAQIERKTET
                     LSDADVVSIVQKEVKKRRDSVEQFEKGGRPELAAKEKQEIAVLESFLPEPLSAEELEQ
                     LVRATIQELGATTKKDMGTVIKAVQAKAAGRADGKTVSGLVGKLLP"
     gene            complement(1666..2115)
                     /locus_tag="VG754_04670"
     CDS             complement(1666..2115)
                     /locus_tag="VG754_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HWF18533.1"
                     /translation="MITPLDLPIVNGSLNGLSAVFLGFGYFFIRNKNVKAHRNCMISA
                     FVTSTLFLACYLTYHYYMARVLHHPTTRFTDPAWFRPIYLTILITHTTLAVVIVPLIL
                     ITLSRALRERFDRHKVIARWTWPLWMYVSVTGVVIYFLLYQIYPQHQ"
     gene            complement(2112..2780)
                     /locus_tag="VG754_04675"
     CDS             complement(2112..2780)
                     /locus_tag="VG754_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWF18534.1"
                     /translation="MNKPARQTEWLVWGGLVLIIAVIGGAFAWTKFAAGRPLPVIGQI
                     SDFTLTNQNNEAVTLSSLRGKVWVADVIFTRCPGPCPTMTHHLAEVQAALPANEPVRL
                     VTLTSDPEYDTPEVLKRYAARFNADSNRWYFLTGPKPEIRRLAVNDFKFVVVEKKPGE
                     QTIPNDLFIHSTWFVLVDQRGRVRGWTDAQGQLHAYFDSEDPAARSEILPAIQQLMRE
                     PTPS"
     gene            complement(2998..3783)
                     /locus_tag="VG754_04680"
     CDS             complement(2998..3783)
                     /locus_tag="VG754_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWF18535.1"
                     /translation="METLYNFTKHVLGLPVLASKHGADVDKLIIYVHYLMAALFIGWI
                     LYFFYVLLRFNSKANPKADYVGIKGHASNYIELVVAVIEGVLLLGFAVPLWAGAVDNF
                     PKAEDHPTNIKIIAQQFAWNVFYPGPDGQFGNTDYSFVTQDNPWGMDKSDPKGKDDFS
                     TLNEIHVPVNKPVIITLTSKDVIHSFKVIAMRVTQDAIPGLRIPLHFTPTQVGVYQVN
                     CAQLCGIGHSSMAGGKLYVDTQEKYDEWLAMKAKGGGAATSFE"
     gene            complement(3786..4067)
                     /locus_tag="VG754_04685"
     CDS             complement(3786..4067)
                     /locus_tag="VG754_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18536.1"
                     /translation="MRRLFGSKIIPALLALAAALPAHSAMACAMCYNSHVDSPLASGM
                     NWGIFSLLAVVVCVLGTIASFFIFLARRSAAVNAASTAPDAALPSTNKA"
     gene            complement(4064..4273)
                     /locus_tag="VG754_04690"
     CDS             complement(4064..4273)
                     /locus_tag="VG754_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18537.1"
                     /translation="MSLKAFHLVFIVASILLAVGFAAWEFVGYFSPHGGAWDLVAGIG
                     GLLTAIGLVFYERYFLKKTKNVSYL"
     gene            complement(4277..4648)
                     /locus_tag="VG754_04695"
     CDS             complement(4277..4648)
                     /locus_tag="VG754_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWF18538.1"
                     /translation="MSDPHDTPAPAQAHAPAPAHAHEDMAHFQQHVKKYLFVFYALIV
                     GTIVTVAASYLPISSVALTVAIALFIACVKGFLVAGYFMHLMSEKKMIYGILLATVFF
                     FAGLMFLIVWSMNKESLIHIH"
     gene            complement(4658..5515)
                     /locus_tag="VG754_04700"
     CDS             complement(4658..5515)
                     /locus_tag="VG754_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWF18539.1"
                     /translation="MEIPYIVEPRPDTGLFNAKVGIWLFLASEVMLFGALFSSYILLR
                     VGAEPGAWPHGLLNIPLGMANTIVLIVSSITVVMSWASLKMKDFSKYKKFQAVTLLCA
                     LTFLCIKSYEYHDKFSHYEVQINPGAVVQAGEGKDVKGITLKAGDFVDGHLLEQDKDK
                     VVIHGHILNKAEAAQVTHAWQQREIKNEEITIKTSDIPLRPDGNLDMENYGPWHSTYM
                     AIYFTLTGLHALHVIGGALVIGFLWGPGASMWKTDPERYTNRIEVSGLFWHFVDLVWI
                     FLFPVLYLL"
     gene            complement(5534..7330)
                     /locus_tag="VG754_04705"
     CDS             complement(5534..7330)
                     /locus_tag="VG754_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWF18540.1"
                     /translation="MQASTTHALPHTGAHEEHHEELGFLRKYVFSTDHKVIGIQYGIT
                     SLLFLLFGFGLMTIMRWSIAHPETRVPIVGSLLEKMLGDVAAGGKVSAALYNSFGAMH
                     GTIMVFLGIVPLAFAAFGNFVVPLQIGAPDMAFPRINMASYQVYVVGGIIMLLSFFMP
                     GGAAQAGWTSYSPLATSIPGYGQTFWLIGMVCLITSSLLGAVNFLATIIQLRAPGMTW
                     MRLPFFVWAQFVTGFLLLLAFPPLEAASVMQLMDKVAHTSFFLPTGLQANGIAHISGG
                     GSPLLWQHLFWFLGHPEVYVLILPAMGIVCEIIANNTRKPIWGYKSLVYSVLVIGFVS
                     FIVWAHHMYLTGMGTRISTFFQTTTMIISIPSVIILTCLFLSLWGGSIRFNTPMLFAL
                     AFLPMFGIGGLTGLPLGFNFSDLHLHDTYYVIAHFHYVVAPGTIFGLFAGIYYWFPKM
                     TGRFMSEFWGKVHFITSFIFMNLIFQPMFAQGMSGMLRRMYDGGINYAATAGTIGGLS
                     QQTIHWNVYISIAAWCLGLAQIPFIINVFWSIKHGKPVTSDNPWDATTLEWQTPTPPP
                     HGNFIKPIIVHRGPYEYSVPGKSKDFTPQTEA"
     gene            complement(7457..>8243)
                     /gene="cyoE"
                     /locus_tag="VG754_04710"
     CDS             complement(7457..>8243)
                     /gene="cyoE"
                     /locus_tag="VG754_04710"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWF18541.1"
                     /translation="VGFYIAYSGPLDLLLLLHTMLGTALVASGAAALNQLLERDYDAM
                     MRRTEDRPLPSGRLQPGTVLILGGAFGGIGLIYLAFAVNLLTSVLGAITLASYLFIYT
                     PLKRVTTLNTIVGAIPGALPPLMGWAAARGHISGGGWSLFAILFFWQLPHFLAIAWIY
                     REQYAQAGFMMLPVVDPAGERTGRQAVSHTLGLLPVSLCPFLFHLAGPVYLVGALVLG
                     LVYLWFAIQFSRQLTISRARQLFFVSILYLPLLLGTMVFDKIK"
BASE COUNT         2117 a         2246 c         2236 g         1644 t
ORIGIN      
        1 gatgtcaaac gaccggcact ttccggccat caacaatcaa caacagacca tcaaccctta
       61 tgtcaaagag ccttcctcct tccaacgaaa cgagagtatc acagccattt tcgaaggcaa
      121 gaaattttgc atcatggtgt agttttcaaa aacaggaatt ctttgtagtt ttcaaaaccg
      181 gacagtttta cttgcggcgc ttggacgcat cgtcttggag ggcctggaaa aggagatcca
      241 tgagaagtgt atcgccctgg cgcgggagcg taaaattgct gcggcgattc gttgacttgg
      301 ctcaaggttg caattagttt ttcggcatga atttcgacat ctctcacctc ctggatcaat
      361 gggattatca gcccggcacg gcgtccgtcc ggaaattcac cgggaaggac ggcgttgaga
      421 aattgcaggt gcggctggat ctggggttgt tgcagatgaa ttgcgagggt cgtccggatg
      481 gcaagcgccc gctgggtcac ccgtcgcttt acgaatatta ccaggcccgg ctcggcaagc
      541 ataagaccgc caatgacggc agcgaggaag gttttctgtt gaaggccgag gattgcgcga
      601 aactgcagtt tgaagcgctg caatattatc agcgccacaa ctgcctctgg caactggaag
      661 attacgccgg cgtcgcgcgc gacacggagc gcaacctgct cgtttgcgat ttcgcggcga
      721 agcacgccga gagcgatgag ttgtcctggt cgttggagca gttcaagccg cagttgctga
      781 tgatgcacac gcgcgcgcgc gccaccgcgt ccttgaagga aaataatcat gaaaccgccg
      841 tgcggcaaat tgacgagggg atggcccgca tccgcgaatt ttatcgcgag cacgaacggc
      901 ttgaaccgga ccaaagtgtc gaactgaatt ccctcaaggc atggctcgat gaaatcaact
      961 cgcgccgccc cttgagcaag cgtgaaaaat tggaacgcga actcagtgaa gccgtcacca
     1021 atgaagatta cgaaaaagcg gcgcagatgc gcgacgcctt gcgcaacctt aaacccaccg
     1081 aatgatcatg agcttgcagg aacgcatttc gcaggaaatc aaaaccgcca tgctcgccaa
     1141 ggacgccgac cgcctttccg cattgcggat gctcaagtcc acgatgggtt acgcgcagat
     1201 cgagcgcaaa acggaaactc tttcggatgc ggacgtggtg tccatcgtgc agaaggaagt
     1261 caagaaacgc cgcgattcag tcgaacaatt cgaaaaaggc ggacgcccgg aactcgccgc
     1321 aaaagaaaag caggagatcg cggtgttgga atcatttttg ccggaaccgt tgagcgcgga
     1381 agaattggag caactggtcc gcgccacgat ccaggaattg ggcgcaacca cgaagaaaga
     1441 catgggtacg gtcatcaaag ccgtccaggc caaagccgct ggccgcgccg atggcaaaac
     1501 tgtcagcggg cttgtcggta aattgctgcc gtaacagaat tgccacgaag aagcacaaaa
     1561 ggaacaaaat ctcagaaaca gaacacgacg cgcgcaacac gatcacctga ttgcgcattc
     1621 aaacccgcaa cgattttttt ttgcgcctct tgtgcctttt cgcggttatt gatgctgcgg
     1681 atagatttgg tagaggagaa aataaatcac cacgccggtc accgagacat acatccaaag
     1741 cggccacgtc cagcgcgcaa tgaccttatg ccgatcaaac cgttcccgca acgcccgact
     1801 caaggtgatc aaaatcaacg gaacaatcac cacggcgagc gttgtgtggg tgatcaggat
     1861 ggtcaggtaa atcggacgaa accatgccgg gtccgtgaac cgcgttgtcg ggtgatgcag
     1921 gacacgggcc atgtagtaat gataagtgag ataacacgcc agaaacagcg tcgaggtcac
     1981 aaacgccgaa atcatgcaat tccggtgcgc cttcacattc ttgttgcgga tgaaaaaata
     2041 accgaaaccg agaaatacag cgctcagtcc gttgagcgag ccgttgacta tgggcaaatc
     2101 gagcggggtg atcatgatgg tgtgggttcg cgcattaatt gttggatggc cggcaaaatt
     2161 tccgagcgcg cggcggggtc ttcggaatcg aaatacgcgt gcaattggcc ttgcgcatcc
     2221 gtccaaccgc gaacgcgccc gcgctggtca accagcacaa accaggtgct gtggatgaac
     2281 aaatcgttcg gaatagtttg ctcccccggc tttttttcga cgacgacgaa tttgaaatcg
     2341 ttgacggcga gccgccggat ttcaggcttg gggccggtga gaaaatacca gcgattggaa
     2401 tcggcgttga aacgcgcggc atatcgtttg agcacctccg gcgtgtcgta ttccgggtcg
     2461 ctcgtgagag tcaccaatcg caccggctcg ttggcgggca aagccgcctg cacttcggca
     2521 aggtggtgcg tcatcgtcgg gcatggcccg gggcagcgag tgaaaatgac atcggccacc
     2581 cacacctttc cacgcaggct ggacaacgtg accgcctcgt tgttctggtt ggtgagggtg
     2641 aaatcggaaa tctggccgat gacgggcagc ggcctgccag cggcaaattt ggtccaggca
     2701 aaagctcccc cgatgacggc gatgatcaac accagtccgc cccaaaccag ccattctgtc
     2761 tgtcgcgcgg gtttattcat gggagatttc agtcgaaaaa ataaattaat ttcggtcaac
     2821 gtcagaataa ttccgttgaa aggggaacgc gcgcgtctcg cgtgcggttc gagccgtccc
     2881 ggcgaggact caacaccaat ttcatgcgga ccagcctcga aaaaagattt caatctcctg
     2941 aatgttcggc gagacgccga acattacacg ccagaggcgt gtgctcccaa aatcccatta
     3001 ctcgaaactc gtggccgcgc cgccgccctt ggctttcatg gccagccatt cgtcgtactt
     3061 ctcctgagtg tccacgtaaa gcttgccgcc cgccatgctc gaatggccga tgccgcaaag
     3121 ctgcgcgcaa ttgacctggt aaactcccac ctgcgtcggc gtgaagtgca acggaatgcg
     3181 caaaccggga attgcgtcct gcgtcacgcg catcgcgatg accttgaacg aatgaatgac
     3241 gtccttcgat gtcagcgtga tgatcaccgg cttgttcacc ggcacatgga tttcattcag
     3301 cgtcgagaag tcgtccttcc ccttcggatc gctcttgtcc atgccccagg gattgtcctg
     3361 cgtcacgaac gaataatccg tgttgccgaa ttggccgtcg ggtccgggat aaaaaacgtt
     3421 ccacgcaaat tgctgggcga tgattttgat gttggtggga tgatcctcgg ctttcggaaa
     3481 attgtccact gcgccagccc aaagcggcac cgcaaaaccg agcagcaaaa cgccttcaat
     3541 gaccgccaca accagctcga tgtaattgga cgcatggccc ttgatgccga cgtaatcggc
     3601 cttggggttc gccttgctgt tgaaccgcag cagcacgtaa aagaagtaaa gtatccagcc
     3661 gatgaacaac gccgccatca ggtaatggac gtaaatgatg agcttgtcca cgtccgcgcc
     3721 gtgcttggaa gccagcactg gaaggcccaa aacgtgtttc gtgaaattat aaagtgtttc
     3781 cattgtcagg ctttattagt tgagggcagc gccgcatccg gcgccgtgga agcggcgttg
     3841 acggccgcgg aacgtctggc gagaaaaata aaaaagctgg cgatcgtgcc cagcacgcaa
     3901 accaccaccg cgagcaggct gaaaatgccc cagttcattc cgctcgcgag aggagaatca
     3961 acatgggaat tataacacat cgcgcatgcc atcgcggaat gcgccggcaa cgccgcggcc
     4021 aacgcaagca gcgcgggaat tattttggat ccaaacaggc gtctcataaa taactgacgt
     4081 ttttcgtctt tttcaaaaaa tatcgttcgt aaaaaaccaa accaatcgcc gtgagcaacc
     4141 cgccgatgcc cgccaccagg tcccatgctc cgccatgcgg cgaaaaataa ccaacgaact
     4201 cccacgcggc aaacccaacc gccagcagga tggaggcaac aataaaaacc agatgaaatg
     4261 ctttcagcga cattgcttaa tggatgtgaa tgaggctttc cttgttcatc gaccagacga
     4321 tcaggaacat cagcccggca aaaaagaaca ccgttgccag caagatgcca tagatcattt
     4381 tcttttccga catcaggtgc atgaaataac cggccacaag aaaacctttc acgcaggcga
     4441 tgaacagcgc aatcgcgacc gtcaacgcca cgctgctgat cggcagataa gaagccgcca
     4501 cggtgacgat tgtcccgaca atcagcgcgt aaaaaacgaa cagatatttt ttaacgtgct
     4561 gttgaaaatg cgccatgtcc tcgtgcgcat gagcgggtgc gggtgcatgt gcttgcgccg
     4621 gtgcgggtgt gtcatgtgga tcgctcataa ttttcagtta caggagataa aggaccggga
     4681 acaggaaaat ccagaccaga tcgacgaagt gccagaacaa tcccgaaact tcaatgcggt
     4741 tcgtgtaacg ctcaggatcg gtcttccaca tcgaagcgcc ggggccccac aaaaagccga
     4801 tgaccaatgc gccgccgatg acgtgcaacg cgtgcagacc ggtcagcgtg aaataaatcg
     4861 ccatgtaagt gctgtgccat gggccatagt tttccatgtc cagattgcca tcgggcctga
     4921 gcggaatgtc agatgtcttg atcgtgattt cttcgttctt gatttcccgt tgctgccagg
     4981 cgtgagtgac ttgagcggct tcagccttgt tgaggatgtg gccgtgaatc accactttgt
     5041 ccttgtcctg ctcgagcaag tgcccatcca cgaagtcgcc cgccttgagg gtgatgccct
     5101 tgacatcctt gccttcaccg gcctggacca ctgcgcccgg attgatctgc acttcgtaat
     5161 ggctgaattt gtcgtggtat tcgtaggatt tgatgcacaa gaaggtcaac gcgcacaaaa
     5221 gtgtgacggc ctgaaatttt ttgtatttgc tgaaatcttt catcttcagc gatgcccacg
     5281 acatcacgac cgtgatgctg gagacgatga ggacgatggt gttcgccatg cccagcggga
     5341 tgttcaacaa tccatgcggc catgcgcccg gttccgcgcc gacgcgcaag aggatgtagg
     5401 aggaaaaaag cgcgccgaac aacatcactt cagacgcaag gaagagccag atgccgacct
     5461 tcgcgttgaa caatcctgtg tccggccggg gttctactat gtacggaatt tccatgaatg
     5521 gtggtcttta aaattaagct tcggtttggg gcgtaaagtc cttcgacttg ccgggcacgc
     5581 tgtattcata cggcccgcga tggacgataa ttggtttgat gaaattgccg tgaggcggag
     5641 gcgtcggggt ctgccactcc aacgtggtgg cgtcccaagg attatcggaa gtcaccggtt
     5701 tgccgtgttt gatgctccag aaaacattga tgatgaaagg aatttgcgcc aagcccaggc
     5761 accaggccgc gatggaaatg taaacattcc agtggattgt ttgctggctc aaaccgccaa
     5821 tcgtgccagc agtcgcggcg taattgatgc cgccgtcata catgcggcgc aacatgcccg
     5881 acatgccctg cgcgaacatc ggctggaaaa tgaggttcat gaaaatgaac gacgtgatga
     5941 aatgcacctt gccccagaat tcgctcatga aacgcccggt catcttcggg aaccaataat
     6001 aaatcccggc gaacaatccg aaaatcgtcc cgggcgcgac cacgtaatgg aaatgcgcga
     6061 tgacataata agtgtcgtgc aggtgcaaat cgctgaagtt aaagcccagc ggcaggcccg
     6121 tcagaccgcc gatgccgaac atcggcagga acgccagcgc gaaaagcatc ggcgtgttga
     6181 accggatgga tccgccccaa agcgacagaa agagacaggt gagaatgatg accgacggga
     6241 tcgaaatgat catcgtcgtg gtctggaaga aggtgctgat cctggtaccc atgccggtga
     6301 gatacatgtg atgcgcccag acgatgaacg atacaaaccc gatgaccagc accgaataaa
     6361 cgagcgattt atagccccaa atcggcttgc gagtgttgtt ggcgatgatt tcgcaaacga
     6421 tgcccatcgc cggcaaaatc aaaacgtaaa cttccggatg ccccaggaac cagaacaaat
     6481 gctgccacag caacgggctg cccccgccgc tgatgtgcgc gatgccgttt gcctgcaacc
     6541 cggttggcag gaagaaactc gtgtgcgcga ccttgtccat caactgcatt acgctcgccg
     6601 cttcgagcgg aggaaacgcc agcagcaaca ggaacccggt cacaaattgc gcccagacga
     6661 aaaacggcag ccgcatccat gtcattcccg gcgcgcgcaa ctggatgatt gtcgccagaa
     6721 agttcaccgc gcccaaaagc gacgaagtga tgaggcaaac catgccaatc aaccaaaagg
     6781 tttggccata gcccggaatg gaagttgcaa gtggcgaata cgaagtccat cccgcttgcg
     6841 ctgcgccgcc cggcatgaaa aagctgagca gcatgatgat gccgccaacg acgtaaacct
     6901 gataactcgc catgttgatg cgcggaaacg ccatgtccgg cgcgccgatt tgcagcggca
     6961 cgacaaaatt gccgaaggcc gcaaacgcca gcggcacaat ccccaaaaat accatgatgg
     7021 ttccgtgcat cgcgccgaaa gagttataca aagccgcact gactttgccc ccggcggcga
     7081 catcgcccaa cattttttcc agcaacgaac ccactatcgg gacccgcgtt tcgggatggg
     7141 caatcgacca gcgcattatc gtcatcaggc cgaagccgaa caacaggaac agaagcgagg
     7201 tgatgccgta ttggatgccg atcaccttgt ggtcggtcga aaaaacgtat ttgcgaagga
     7261 aacccagttc ctcgtggtgc tcttcgtgtg cccccgtgtg cgggagcgcg tgagtggtgg
     7321 atgcctgcat acttatgatt tcaattgctt gtttagcttc tcgagcacta ttacttgtcc
     7381 taactcagcc aaattatgct gtcttacaat aacttttctt tgtcatcggt gtcaacgtca
     7441 gaaaaccgaa attagcttac ttaatcttat caaataccat tgttcctaac aacagaggca
     7501 gatataaaat cgaaacaaaa aataattgcc gcgcgcgtga aattgtcagt tgtcgcgaaa
     7561 attggatcgc gaaccacaaa taaaccagtc ccaacaccag cgcgccgacc agataaaccg
     7621 gcccggcaag gtgaaaaaga aatgggcaaa ggctcaccgg cagcaatcca agcgtatgac
     7681 tgaccgcctg acgaccggtt cgttccccgg ctggatcgac cactggcagc atcatgaagc
     7741 cggcttgcgc gtattgctcc cgataaatcc aggcgatcgc cagaaaatgc ggcagttgcc
     7801 agaaaaaaag aatcgcaaac agcgaccatc cgccgccgct gatgtgcccg cgggcggccg
     7861 cccagcccat caatggcggc aatgcgcctg gaattgctcc aacgatggtg ttaagcgtcg
     7921 tcacccgttt gagaggcgtg taaatgaaaa ggtaactggc cagtgtgatt gcaccaagca
     7981 cgctggtgag caggttcact gcgaaagcca gataaatcag accgatgccg ccgaatgctc
     8041 cgcccaaaat caacactgtg cccggttgca atcgcccgga tggcaacggg cgatcttccg
     8101 tgcggcgcat catggcgtcg taatcgcgtt ccaacaattg attcaaggcc gccgcgccgc
     8161 tggccaccag cgctgttccc agcatcgtgt gcagcaacag cagcaaatcc agcgggccgg
     8221 aataggcaat gtaaaagcca acc
//
LOCUS       DASZMM010000197         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1728604, whole genome shotgun sequence.
ACCESSION   DASZMM010000197 DASZMM010000000
VERSION     DASZMM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728604"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            623..>2210
                     /locus_tag="VG754_04715"
     CDS             623..>2210
                     /locus_tag="VG754_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18542.1"
                     /translation="MRNPKIEGRKQYQPSGKINWAKFFPFALLSFFAAVVLAVILAVL
                     FNVGFYLILLVPILAGLGLGGTVLLAVKMGHCRSPMKGALLGAVGGIILYLGYYYIGM
                     EQTLGRLNPGDIFLLPRYIKVRMDYTVIKDVHTPDSNKDDDQKPTGFRTGSNWLFFAL
                     EFGFIVGIAAGSGLRWSRKPYCENCRTWMSRETTTFTPEVGPGFVEALRLGSVQSLAA
                     LFTSPQKPSAPHTAVAVDYCPRLKEGAAKDCDIYLSVKQVVAAAKGVTLNSFDQSKGK
                     FLAQQVLLNRTEVPAVLVRFPSLESVAGTTAAQALQELQVEMPPAPSPHAMAEVMPVD
                     PLHAKKVLTTKTGLIVTAFTLMAFLGVFVAIGLGALGGYLGFPQPAPAGGVPIVMKIL
                     GSILIGVAILIFVTTLVLVFTSPGYFGSRYLRKLTETEFRKRSKPLVSTADADARFVQ
                     VIPRANWAKLKLIDATDIGFLRVDKQRGELLFEGDKENYRVPAAAITSCEIETFIQGQ
                     GSHGATTLYRVVLQANHPSGF"
BASE COUNT          476 a          640 c          560 g          534 t
ORIGIN      
        1 agcgcgtttg atacttcagg ttgttttcgc ggtcgtccat atctaaaatt cctcgacaac
       61 ctgctgttcc cctgtcacaa tccgtcgcgt gtggaaacaa ggagcacaca gggacacctc
      121 caatgtccgg caatccggtt catccttcaa ccggacggga atcaatttgt ttgcccaaat
      181 gctgcatttg ccgtgttcgc gcccaaaaca gaaccacatt tcagcggcac atgccgcata
      241 tgaatccgat gcgtaagtac ttgaaaaccc gcttattccc acatagaccc gcttttcttc
      301 acataaatcc ggcccctccc ccccgggaat atgacgacat gagttcggca atccgaccat
      361 cctcaatccg actcaacacg gaaccaacct atcaccggga gatccgcatc tgcccattcg
      421 taagtcattg caaacccgcc tattcccaca taaacccgct tttcctcaca taaattcgcc
      481 ccctcccccc catcctatat gtcacgcaac cgaaatagtt ttacccttga gccgcagctc
      541 gtgttttcct aacctctggc acacataaca ggaagccttt cagttcaaca tgcgacaacc
      601 atctccatga tttgatatgg ccatgcgaaa tccaaagatt gaaggcagga aacaatatca
      661 acccagcggc aaaatcaatt gggcgaagtt ttttccattc gctctgttga gctttttcgc
      721 ggcggtcgtg ctcgccgtca ttttggccgt tcttttcaat gtcggttttt atctgatact
      781 gctggtgccc atcctggcag ggctcggact tggcggcaca gtattgcttg ccgtaaaaat
      841 gggacattgc cgcagcccga tgaagggagc gttgttgggc gcggttggag gaatcattct
      901 ttatcttggt tattattaca tcggcatgga gcaaacgctt ggaaggctga atcccggaga
      961 catcttcctg cttccgcgat acatcaaggt caggatggat tacacggtca tcaaggacgt
     1021 gcatacgccg gattcaaaca aagatgacga ccaaaaaccc accggctttc gcaccggctc
     1081 caattggttg tttttcgcgc tggaattcgg atttatcgtc ggcatcgccg ccgggagtgg
     1141 actgcgatgg tcgcgcaagc catattgcga gaattgccgg acgtggatga gccgcgaaac
     1201 gacgacgttc acgccggaag tgggtcccgg ttttgtcgag gcgttgcgtc ttggctccgt
     1261 gcaatcgctc gcggcgttgt tcacttcgcc gcaaaaaccc tcggcgccgc acacggcggt
     1321 cgccgtggat tattgcccgc gtctcaagga aggtgcggcc aaagattgcg acatttatct
     1381 gtcagtgaaa caagttgtgg ccgcggcaaa aggcgtgaca ctgaattcgt tcgatcaatc
     1441 caagggaaaa tttcttgcgc aacaagtcct gctgaaccgg actgaagttc ccgccgtgct
     1501 tgtccgtttc ccgtcgctcg aaagtgtcgc gggcacgacc gcggcgcagg cgttgcagga
     1561 acttcaggtg gaaatgccgc ccgctccttc gccccacgcc atggcggaag tcatgcccgt
     1621 tgatccgctc catgcaaaaa aggtgttgac caccaagacc ggtttgatcg tgacggcatt
     1681 cacccttatg gcatttctcg gtgtgtttgt ggccatcggt cttggcgcgt tgggaggcta
     1741 tctcggtttc ccgcaacccg cgccggccgg aggagtcccg attgtgatga aaattctcgg
     1801 ctcgattttg atcggcgtgg ccattttgat tttcgtgacc accctcgttc tcgtcttcac
     1861 ctcgccgggt tatttcggct cgcgttacct gcgcaagttg acggaaaccg agtttcgcaa
     1921 gcgcagcaag ccattggtca gcaccgccga tgccgatgcg cgttttgtcc aggtcattcc
     1981 ccgcgccaat tgggccaagc tcaaattgat tgacgcgacg gacatcggtt ttttgcgcgt
     2041 cgataagcag cgcggcgaac tgctcttcga gggcgacaag gaaaattatc gcgttcccgc
     2101 ggcggcgatc acttcctgcg aaattgaaac gttcatccag ggccaaggct cgcatggagc
     2161 gacgactctt tatcgcgtgg tcttgcaagc gaaccacccc tccggatttt
//
LOCUS       DASZMM010000198         5608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3379901, whole genome shotgun sequence.
ACCESSION   DASZMM010000198 DASZMM010000000
VERSION     DASZMM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379901"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            601..2316
                     /locus_tag="VG754_04720"
     CDS             601..2316
                     /locus_tag="VG754_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWF18543.1"
                     /translation="MRTILITGFFAFALTFAAQAIDDYKPGPDSMPQEGVPKGEVTKY
                     AFDHSKIFPGTTRDYWIYVPKQYDPATPACVMVFQDGIQYKAPVVFDNLIAKKEIPVI
                     IGVFVMHGRVKALSTNANDRFNRSYEYDGLGDNYARFLLEELLPEVAKKYNLSTNAND
                     RAIAGNSSGAIAAFTAAWERPDAFRRVFSGIGTYVGLRGGNNYPTLIRKTEPKPIRVF
                     LQDGSNDLNIYGGNWHLANQEMLSALEFAGYEVNHEWGDGRHSGVHSTAIFPEALRWL
                     WKDWPVPITTGNNSKQPLFDILIPDEGWQIVSEGHHFVDGPTANAAGEVFFNDIGNKR
                     IYKVGLDGKVSLFTEGHPDCAGLMFGPDGKLYGCQIGARRIVSFAADGKEEVIAKDTD
                     CNDLAVTHTGEIYFTDHINKKIWFINAQHEKRLVDEGIAFPNGIRLTPDQGLLQVSDT
                     KGQFVYSFQVEPDGSLVDKQRYFDLHIVDGAMQSGADGMTVDTEGRLYVTTEMGIQIC
                     DQAGRVQCIIPKPARAGLPAVAFGGANRDELYVACGDKLYKRKTKAKGVLSFEAPIKP
                     PAPHL"
     gene            complement(2414..3022)
                     /locus_tag="VG754_04725"
     CDS             complement(2414..3022)
                     /locus_tag="VG754_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18544.1"
                     /translation="MILEHLIHHAILFCVTIRFRLRLVIKKVGMPKAGPAAAERQQLI
                     QKMKSTKLGIIAAIALGGLMACGPIALAQDKPATPPASGDNSTPPPRGPRGRGNPMQG
                     ILNKLDLTADQKEKVKPIVEDQGKQMRALFQDNSLSQEDRQAKRKSIMDATDAKLKDI
                     LTPDQFTKYQDMRKQMMGRRGQRGPGAGGPPAGSTPPKSDSQ"
     gene            complement(3168..4745)
                     /locus_tag="VG754_04730"
     CDS             complement(3168..4745)
                     /locus_tag="VG754_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HWF18545.1"
                     /translation="MQGYLSLILHAHLPFVRHPEHPKFLEENWLFEAITETYIPLIQI
                     MDGWLRDGMDTRLTLTLSPTLCSMLLDPLLQDRYQRHLEGLIELAEKEIHRTHWDDAF
                     RPLAWMYHHRFTNTRNTWFAYGRNLVGAFRKFQEHGKLEIITTAATHALLPLLANHPP
                     SIRAQILVARDHYRSCFGCDPRGIWLPECAYVEGVEHILQEANIRWFIIDTHGILHAR
                     PRPRYGVFAPIFTPNFIAAFGRDLDSARQVWSKHEGYPGDVRYRDFYRDIGFDLDFDY
                     VKPYLPDLSQRGFTGLKYHRISGDAPEKQIYDRDAALKVAAEHAGHFLGERMNQIQRL
                     AGIMDRPPVIVSPYDAELFGHWWYEGPEFLDYFMRKAIYDQQVIRLATPQEYLNRQPT
                     QQIARPSASSWGSEGYWSVWLNETNEWIYPHLTIAQERMTQLARKFPHATGLQLRALK
                     QAGRELLLAQSSDWPFILRTGTSPDYARKRVKDHLLRFIAIYEKLTAASQIDEPWLAR
                     IEAMDNIFPDINYHYWE"
     gene            complement(4902..5132)
                     /locus_tag="VG754_04735"
     CDS             complement(4902..5132)
                     /locus_tag="VG754_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18546.1"
                     /translation="MSEYSRMATTIVRLVAGGFLVVGFLNLGLEGFKSHHDHMPLNVW
                     RCLYLSIPLVVGVVILIKCSALARWVDRYLDE"
     gene            complement(5134..5541)
                     /locus_tag="VG754_04740"
     CDS             complement(5134..5541)
                     /locus_tag="VG754_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18547.1"
                     /translation="MERDAEHLKLLSMFHYIRGGICAVFSCFFAVYIVAGMIIAMTSA
                     FSHARNAPPPALGLVLALAGGCIVLVGWTWGALQIYAGHCLAQRKHRMYCMVIAGLSC
                     LFIPYGTLLGIFTLMVLQRPSVRQTFEQPPAVK"
BASE COUNT         1310 a         1509 c         1523 g         1266 t
ORIGIN      
        1 tgcaagaaat tagccggggg taaggccgca ggccgccacc cccggttgtc tgttgttcaa
       61 ttttccaccc tgaaagggtg gcagaaacgc gattggttcg ttggttctgg caccccttca
      121 gggtgcatcg gtttacgcga cattttccgg gggtgtcgct tcgctcaacc cccggctaat
      181 ttcttgcaac cctccgggtt gcttcagcca gccagctcgt cttcgagatg cctcggaacg
      241 aaaacatcca tttcgcgcga ccagacactt tgtgtctcgc caatctccgg tgctccttcg
      301 gagcacagag catctttgcg cctttgcgtt aaatccacgt attttccgcc atcgtcgcgg
      361 agaaatttcc gcttaattcc gcttgtttcc accccgttcc gcttaattcc tgataaaaaa
      421 attaattttt ttcgcgaaat gaatcccatt tccctccatc acatgctgct tccccgcatg
      481 aaaattccga tgctcaaatc actaattatt cctgatgcat ctgacacatc ctcatttatg
      541 aaacgtgcgc aatattttaa tggaacttct ttgtccccac gccataatcc ggccatcact
      601 atgcgcacga ttcttatcac tggttttttt gcttttgccc ttacttttgc cgcccaggca
      661 attgacgatt acaaacccgg acccgattcc atgccgcaag aaggcgtgcc gaagggcgaa
      721 gtcaccaaat acgccttcga ccacagcaaa atttttcccg gcacgacgcg cgattattgg
      781 atttacgtcc ccaaacaata cgatcctgcc acgcccgctt gcgtgatggt ttttcaggat
      841 ggcatccaat acaaggcgcc ggtggtgttc gacaatctca tcgcgaagaa ggagatcccc
      901 gtcatcatcg gcgtgttcgt catgcatggg cgcgtgaagg cgctctcgac gaatgcgaat
      961 gaccgtttca atcgcagtta tgaatacgat ggcctgggcg acaactacgc gcgctttttg
     1021 ctggaagaat tgctgcccga agtcgccaaa aaatacaacc tcagcaccaa cgcaaacgac
     1081 cgtgccatcg ccgggaacag ttcgggggcg attgcggcgt tcacggcggc gtgggagcgg
     1141 cctgatgcgt tccggcgcgt tttcagcggc atcggcacgt atgtcggttt gcgcggcggg
     1201 aacaattatc ccacgctcat ccgcaagacg gagccgaaac cgatccgcgt ttttttgcag
     1261 gacggctcga atgatctgaa catctacggc ggcaactggc atctggccaa ccaggaaatg
     1321 ctctcggcct tggaatttgc gggttatgaa gtcaaccatg aatggggcga cgggagacat
     1381 tcgggcgttc attccacggc gatttttccc gaagcgttgc gctggttgtg gaaggattgg
     1441 cccgtcccaa tcacgaccgg caacaactca aaacagccgc tgtttgacat cttgattcct
     1501 gacgaaggct ggcaaatcgt gagcgaaggg catcattttg tggacggccc aacggccaat
     1561 gccgccggtg aagttttctt caacgacatc ggcaacaaac gcatttacaa ggtcgggctt
     1621 gacggcaagg tgtcgttgtt caccgaaggc catccggatt gcgcggggtt gatgttcggc
     1681 ccggatggaa aactttacgg ttgccagatt ggcgccagac gcattgtctc cttcgctgcg
     1741 gacggcaaag aagaggtgat cgccaaggac accgattgca acgacctcgc cgtgacgcac
     1801 accggtgaaa tttatttcac cgaccacatc aacaaaaaaa tctggttcat caatgcgcaa
     1861 cacgaaaaac ggctggtgga cgaaggcatc gcgtttccaa acggcatccg gctcacgcct
     1921 gaccaaggcc tcttgcaggt ttccgacacg aaaggccagt tcgtttattc attccaggtt
     1981 gagccggacg gttcgcttgt ggacaagcag cgctattttg atctgcacat cgtggacggt
     2041 gcaatgcaaa gcggcgcgga cggcatgacc gtggacacgg agggacgttt gtatgtgacg
     2101 accgaaatgg gaatccagat ttgcgatcaa gccggacgcg tgcaatgcat catccccaaa
     2161 ccggcgcgtg cgggtttgcc agccgtcgcg ttcggcggcg cgaaccgcga tgaactttac
     2221 gtcgcgtgcg gcgacaaact ttacaagcgc aagacgaagg ccaaaggtgt cctgagtttc
     2281 gaagccccta tcaaaccacc cgcgccgcat ctttaaaaaa gtttaaagct ttcgcgccca
     2341 tgaatatcaa aaacaaaaag ggacggtctc caacggaccg tccctgtcta acacacaacc
     2401 tgcgggggca aaattattgg ctgtccgatt ttggcggtgt tgagccagca ggaggaccac
     2461 cagcaccagg gccacgttga ccgcgccggc ccatcatctg tttgcgcata tcttggtact
     2521 tggtgaactg gtcgggcgtg aggatgtctt tgagtttggc atcggtcgcg tccatgatgg
     2581 atttccgctt cgcctggcga tcttcctggg aaagcgagtt gtcctgaaac aaagccctca
     2641 tctgtttgcc ttgatcttcc acgattggtt tgaccttttc cttttggtcg gccgtcaggt
     2701 cgagtttgtt caagattccc tgcatcgggt tgccgcgacc acgaggaccg cgtggcggag
     2761 gagtcgaatt gtcaccgctt gcgggaggag tggcgggttt gtcctgcgcg agcgcgatcg
     2821 gtccacacgc catcaatccg cccaaagcga ttgctgcaat gatacctaat tttgttgatt
     2881 tcattttttg tataagttgc tgtcgttctg ctgcggccgg acctgccttt ggcatgccga
     2941 ccttctttat taccagacgc aaacggaagc gaatagttac acagaataag atggcatggt
     3001 ggattaaatg ttccaaaatc aaacatccga cttgcaaagc actgatagaa aacaggttga
     3061 accgcgtcta tttagtccga ttcgaagacg acgtccgcga tgtgagaaga gttttaatta
     3121 aggcgtctaa tcgaaacttg gccttgcagc ttcggaacga agaaccgcta ttcccaataa
     3181 tggtaattga tgtcggggaa aatattgtcc atggcttcga tgcgagcgag ccatggttca
     3241 tcaatttgcg aagcggcggt gagtttctca tagatcgcga tgaagcgcag cagatggtcc
     3301 ttgacccgtt tgcgagcgta atcgggactt gtgccggtgc gaaggatgaa cggccagtcg
     3361 ctcgattgcg cgagcagcaa ttcgcgtccg gcttgtttga gcgcgcgcaa ttgcaacccg
     3421 gttgcgtgcg ggaattttct tgccaattgg gtcattcgtt cctgcgcgat agtcaagtgc
     3481 ggataaatcc attcgttggt ttcgttgagc cagacgctcc aataaccttc cgaaccccag
     3541 ctcgacgcgc tgggccgcgc gatttgttgc gtcggctgac ggttcaaata ttcctgcggc
     3601 gtggccaggc ggatgacttg ctggtcgtaa atggctttgc gcataaaata atccaggaac
     3661 tccggccctt cataccacca atggccgaac aactcggcgt catacggcga cacgatgacc
     3721 ggcggacgat ccatgatgcc ggcaagacgc tggatctggt tcatgcgttc gccaagaaaa
     3781 tggcccgcat gttccgccgc aactttgagc gcggcatcgc ggtcatagat ttgtttttcc
     3841 ggcgcatcgc cgctgatgcg atgatacttg aggccggtga aaccgcgctg gctcaaatcc
     3901 ggcagatacg gtttgacgta atcaaaatcc aagtcaaaac cgatgtcgcg gtaaaaatcg
     3961 cggtaacgga catcgccggg ataaccttcg tgtttgctcc agacttgccg cgcggaatcg
     4021 aggtcgcgac cgaatgcggc gatgaagttc ggcgtgaaaa tcggcgcgaa cacgccgtaa
     4081 cgcgggcgcg ggcgcgcatg caaaatcccg tgggtatcaa tgatgaacca gcggatgttc
     4141 gcttcctgca aaatatgttc cacgccttcg acgtaagcac attcaggaag ccaaatgccg
     4201 cgcgggtcgc agccgaagca actgcgataa tggtcgcgcg cgacaagtat ctgcgcgcgg
     4261 atggagggcg gatgatttgc gagcaacggc agaagcgcgt gcgtggcggc ggtggtgatg
     4321 atttcgagct tgccgtgttc ctggaatttg cggaacgcgc cgacaaggtt ccggccataa
     4381 gcgaaccagg tattccgggt gttggtgaac cgatgatgat acatccacgc gagcggacga
     4441 aacgcgtcgt cccaatgggt gcgatggatt tccttttcgg cgagttcgat cagcccttca
     4501 agatggcgtt ggtagcggtc ctgcaacaac gggtcgagaa gcatcgagca gagcgtcggc
     4561 gaaagtgtga gcgtcaggcg cgtgtccatt ccgtcccgca gccagccgtc catgatttgg
     4621 atgagcggga tgtaagtctc ggtgatggcc tcgaagagcc aattttcttc gagaaacttc
     4681 ggatgctccg gatggcggac gaaagggaga tgcgcgtgga gaattaggga aagatagcct
     4741 tgcatgatca gagaacccaa acctccaacg ttgaacgtcg aacgtcaaac atcgaattga
     4801 acgcggagaa gaggctgtct ttcattcgat gttcagtgtt cgacgttgga tgttcgatgt
     4861 tcccattttt tcctcgcata acaatccaaa acccattccc attattcatc caaatatcga
     4921 tccacccaac gggccaacgc cgaacacttt atcaatataa ccactccaac cacaagcgga
     4981 atggaaagat aaaggcagcg ccagacattc aagggcatat gatcatgatg acttttgaac
     5041 ccttccaaac caagattcaa aaaacccaca accagaaaac cgcccgcgac caggcggaca
     5101 atcgtagtgg ccattcggct gtattcgctc atatcatttg actgcgggcg gttgttcaaa
     5161 tgtttggcgc acggacgggc gttgaaggac catcagcgtg aaaatcccaa gaagcgtgcc
     5221 atacggaatg aacaggcaac tgagtcccgc aatcaccatg caatacatgc gatgcttccg
     5281 ttgcgcgagg caatgtccgg cgtaaatctg caacgcgccc caggtccaac ccacaagcac
     5341 aatgcaaccg cctgccaatg cgagaaccaa acccaatgcc ggcggaggcg cattccttgc
     5401 gtgtgaaaat gcggacgtca tcgcgattat catgcccgcc acaatgtaaa cagcgaagaa
     5461 acaggaaaag accgcgcaga ttccgccccg gatgtaatgg aacattgaga gcagctttag
     5521 atgttcggca tcgcgctcca ttgctgtctg cgcttatgcc acgctggccg caagcgggag
     5581 tttgagttgc ggatcttgcg ggtgcgcg
//
LOCUS       DASZMM010000199         8427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4467446, whole genome shotgun sequence.
ACCESSION   DASZMM010000199 DASZMM010000000
VERSION     DASZMM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4467446"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..868
                     /locus_tag="VG754_04745"
     CDS             59..868
                     /locus_tag="VG754_04745"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWF18548.1"
                     /translation="MRLPNPKQERRLTLLVWLGIAFGLVLVAVSCSTDRTVVIPLEIE
                     GATYVGDKACADCHANITRAFPASPHARLHLDRDGMSGQNGCESCHGPGSKHIAQGGR
                     GGLDKFIINPGKNPAGCFQCHPEAQAEFHLPQHHPLTEGKMNCVQCHDPHGLDIMKPA
                     GGLTLSRLNETCAQCHREQTKPVSFDHPAMREECTACHSPHGSVNLKLLTVPDSNLCF
                     RCHAQVQGPGAQGHVFIGGRDHTSSLPFGTCWTSGCHTAVHGSNIHPILLY"
     gene            877..3243
                     /locus_tag="VG754_04750"
     CDS             877..3243
                     /locus_tag="VG754_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HWF18549.1"
                     /translation="MNSIAARRQICLGFFLSLACARAGAAEVDVSKLPPPAGVQVNFS
                     RDIQPIFETSCLRCHGPEKPKSKFRLDNRVAALKGGDDGVDILPGNSAKSPLIHYVAG
                     LVEDMEMPPQGKGKKPTTQQISLLRAWIDQGAVWSTANPTNSADFTLSTIFGGTSVSG
                     DKHKFREHYWQKEGPNGGIEQFELFEQTGPDTRLLMTGHALVDDYKVEFDVDRNELGF
                     VHAGWEDYRKYFDDSGLYDPFLVPTAPRLREDLHLEIGKAWIDFGLALPDWPLLVFGY
                     EYDYKKGNEAMTQYGNVGTRPNTARNIDPASKAMNEHVHIIKFDLNHEIGGVSVEDRF
                     RGEFYVLRTDYTNVAFGQIAQKVNEGTSYFQGANTLRLEKKFNDWFFASAGYLFSKMD
                     ADSSFNLDYPTRFQTAGIPSIALERDSRVGNLNGLFGPFDGLTISTAAQAEWTHQHGF
                     GLGTLDQRSPPPPPFTDTIVPFYVASDYDETSVKENLALRYSKIPFTSVFAEARLQEE
                     KIGQFDQFSSSQDIINKAVFLQRTAFSSLLSDARAGFNTSPWRVVTLSGHVRRYEDDS
                     HYNSDALVQPVQTAYPTFIRSRDLLTDEVEGRIALRPATWFKTSVTYQYQKTDYQMDT
                     LPFVSFGNVISPGGPVSSGEDASQTVSLNATFTPSSRLYLSSTFSWQTFDAVTADQGS
                     PAVAPYRGDTYSALADATFVLDESTDLFVNYAFSQSDYAQNNFVAGVPIGIEYQRHST
                     QVGLAHRFNKRVSAKLQYRYDYYDEPTSGGAANYRANAAFCIFTFRFR"
     gene            4337..4906
                     /locus_tag="VG754_04755"
     CDS             4337..4906
                     /locus_tag="VG754_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RfaH"
                     /protein_id="HWF18550.1"
                     /translation="MTQPAWYCARTKPKHEHIAAANLRKHLGLEVFNPQLRVERSTRR
                     GLVRTVEPLFPCYIFVRCAIQERLDDIRYTNGISSIVHFADRIPAVSDSVIAELKSCF
                     NSGEPMPVEEKLSPGAEVVLGEGAFSGMKASVLRVMPAGRRVQVLLDILGRPTAVEVD
                     RRFVALEKNTMADLVPLLAAPPRPVFVRA"
     gene            5063..6148
                     /locus_tag="VG754_04760"
     CDS             5063..6148
                     /locus_tag="VG754_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWF18551.1"
                     /translation="MRTQAPEDADFLAMEHGFLQLAEAIGSVLKMTDAEDGRTLYISP
                     GFEEIWGRSCEDVRNSTRAWYDTIHPEDRDRMLDAGLGIRRHGGFDEEYRIIRPDGSM
                     RWVRERAFGIRNAAGKVSQAASITEDITERKLLEKEVIENNDRERAQLGRDLHDGICQ
                     QLVSIAFATDLLRRDLAAQLPGEAVRAGKVTSLLDNAITQARNLSHALCPVNLAGDGL
                     AVALRSLAGAVNKGFGVVCGADCSEKVFIHDHAVATHLYRIAQEAVQNAVQHSHPTQM
                     LIRLTQEGETVRLSVTDNGKGADEEGERVFAVGLNIIKFRTQMTGGRLKVERSAAGGT
                     TISCMFQQKPPGQRKTETDFQLLHAKA"
     gene            6240..7058
                     /locus_tag="VG754_04765"
     CDS             6240..7058
                     /locus_tag="VG754_04765"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWF18552.1"
                     /translation="MESLADIKSKDPAPIAPGPREVPRWKRVLDTALILLAMPLLLPL
                     MLFIAVLVRCVSAGPVLFRQERVGHLGSRFRCFKFRTMQVGNNAAVHQGHLKELMDSN
                     APMVKMDVKGDARIIPFGVPLRASGLDELPQIFNVLFGQMSLVGPRPCVPYEYDSYLP
                     WQKERFDVLPGLTGFWQVSGKNNTTFEEMMRLDIYYARNSSVGFDLRIIFKTVPALLI
                     QMFETRRRRKAEQRATLNGGGGGAATSPALLRSGTNLPTTQFKMTARTKNQEAL"
     gene            7055..8233
                     /locus_tag="VG754_04770"
     CDS             7055..8233
                     /locus_tag="VG754_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF18553.1"
                     /translation="MTKQIKIGVVGLGYWGPNLVRNFRALPDCDLRMMCDLSEERLDH
                     LTSLYPEVEAASDFKHMLNGANLDAVVIATAVRSHYPMAKASLLAGKHTFIEKPMASS
                     SEQCEELIDIARRKGLVLMTGHTFLYSPAVRKIKELVDSGDIGEIRYISARRLNLGLF
                     QKDINVAWDLAPHDISIIQHIIGEQPVTVNCRGSAHITPGIEDVTTMCLSFEKQRTAI
                     IHSSWLDPRKVREMTIVGSKRMIVYDDVMQQEKIKIFDARVERPPHYDTFAEFHYAYH
                     YGDVYAPYLKQEEPLKIECQHFLDCIKNGSAPLTSGERGLDLVRILEASSESLKLGGG
                     PVELAPSLAVKVAFNPAAVRNGNGHRNGNGNGNGNGHAVAAKKRGARPTAPKVAVNGR
                     "
     gene            8265..>8427
                     /locus_tag="VG754_04775"
     CDS             8265..>8427
                     /locus_tag="VG754_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWF18554.1"
                     /translation="MSHQVFQRIAPDVKLGKNVRIYDFTNLYGCEIGDDVKIGTFVEI
                     QRGAKIGNAC"
BASE COUNT         1958 a         2131 c         2387 g         1951 t
ORIGIN      
        1 tctccgcttg gaaccataga cccggttgaa accaaccacc cttcagttag cgaaacagat
       61 gcgtttgcca aatccaaagc aggaacgccg gctgacctta ctggtctggc ttgggattgc
      121 atttggattg gtgctcgtcg cggtttcctg ttcaacggac aggacggtgg tcattccgtt
      181 ggagatcgaa ggtgcgactt atgtggggga caaagcatgc gccgattgtc acgccaacat
      241 cacgcgcgct tttcccgcga gcccgcacgc gcgtttgcat ctggatcgcg acgggatgtc
      301 cggtcagaac ggttgcgaat cgtgccacgg tcccggcagc aaacacatcg cgcaaggggg
      361 caggggcgga ttggacaagt tcatcatcaa cccgggaaaa aatcccgcgg gctgttttca
      421 atgtcatccc gaagcgcagg cagaattcca tcttccgcag caccatccct tgacggaagg
      481 aaagatgaac tgcgtgcaat gccacgatcc gcacggcttg gacatcatga aacctgcggg
      541 cggtttgacg ttgtcgcgat tgaatgagac ttgcgcgcaa tgtcatcgcg agcagacgaa
      601 gccggtctcc tttgaccatc ctgcgatgcg cgaagagtgc accgcgtgtc acagtccgca
      661 cggctcggtc aacctcaagc tgctcaccgt gccggattca aacctctgct tccgttgtca
      721 tgcacaggtg cagggaccgg gcgcgcaagg ccatgtcttt attggcgggc gcgaccacac
      781 ttccagtctt ccatttggga cgtgttggac atcgggatgc cacacggcag tccacggctc
      841 gaacatccat ccaatccttc tctattgaaa agagggttga acagcattgc ggcgcggaga
      901 caaatctgtt tgggattttt tttgtcgctt gcgtgcgcac gagccggcgc ggctgaagtg
      961 gacgtttcca aactgccgcc accggcgggt gttcaggtca atttttcccg agacatccag
     1021 ccaatcttcg aaacgagttg tcttcgctgc catggcccgg aaaaaccgaa gtccaagttt
     1081 cgtctcgata accgggtggc cgcgctcaag ggcggtgatg atggtgtgga catcttgccc
     1141 ggcaacagcg ccaaaagccc gctcatccac tatgtggcgg ggctggttga agacatggaa
     1201 atgccgccgc agggaaaagg aaaaaaacct acgacgcagc agatttcgtt gttgcgcgca
     1261 tggattgatc agggagccgt ctggagcacg gccaacccga cgaacagcgc tgatttcacc
     1321 ctttcgacaa tttttggcgg cacaagcgtc agtggcgaca aacataaatt ccgcgagcac
     1381 tactggcaaa aggaaggacc caatggcggc attgaacaat tcgaattgtt cgaacaaaca
     1441 gggcccgaca cgaggctgct gatgaccggc catgcgttgg tcgatgatta taaggttgaa
     1501 tttgacgtgg accgaaacga actcggattt gtccatgcgg gttgggaaga ttatcgaaaa
     1561 tatttcgacg acagcggcct ttatgatcca tttctggtgc caaccgcacc caggcttcgc
     1621 gaagatttgc acttggagat cggcaaggct tggattgatt tcggcctggc attgccggat
     1681 tggccgctgt tggttttcgg ttacgaatat gattacaaga agggcaatga agcgatgacg
     1741 caatatggca atgtgggcac gcgtccgaac accgcgcgca acatcgaccc ggcaagcaag
     1801 gcgatgaatg agcacgttca catcatcaaa ttcgacctga accatgaaat cggcggtgtg
     1861 agcgtcgagg accgttttcg cggagaattt tacgtgttga ggaccgatta cacgaacgtc
     1921 gctttcggcc agatcgccca aaaagtgaac gagggcacgt cctattttca aggcgcgaac
     1981 acgctccggc ttgaaaagaa attcaacgat tggttcttcg cctccgcagg ctatcttttc
     2041 agcaaaatgg atgcggactc gtcgttcaat ctggattatc cgacacgctt tcaaaccgca
     2101 ggcatcccat cgattgcttt ggaacgagat tctcgcgtgg gaaatctcaa cgggcttttc
     2161 ggcccgttcg atggcctcac catttcgacc gccgcgcaag cagaatggac gcaccaacat
     2221 ggcttcggct tgggaacact cgaccagcga tcgccaccgc cgccgccgtt caccgataca
     2281 atcgttcctt tttacgtcgc ttcggattat gacgagactt cggtgaagga gaatttggcg
     2341 ttgcgttatt ccaaaattcc gttcacatcc gtttttgcgg aagccaggtt gcaggaggag
     2401 aaaatcgggc aattcgacca gttctcctcg tcgcaggaca ttatcaacaa ggcggttttt
     2461 ctccagcgca cggctttttc cagcctgttg agcgatgcgc gcgcgggttt caatacttct
     2521 ccgtggcggg tggttacgtt gagcgggcat gtccgccgct acgaagacga ttcgcattat
     2581 aacagcgatg cgctggtgca accggtccag acggcgtatc cgacgttcat tcgttcacgc
     2641 gatctgctga ccgatgaagt cgaggggagg attgcgttac gtcccgcgac atggttcaag
     2701 acctcggtga cttatcagta tcagaaaact gattatcaga tggacacact tccgtttgtg
     2761 agcttcggca acgtcatctc tccgggcggg ccggtttcct cgggcgaaga tgcctcccag
     2821 accgtttcgt taaatgcaac tttcacgccg agttcgcgcc tttatctttc ctcgacattc
     2881 tcctggcaga ccttcgacgc ggtgaccgcg gaccaaggtt cgccggcagt ggctccctac
     2941 cggggcgata cgtattcggc gcttgccgat gcgacgtttg tccttgatga gtccaccgac
     3001 ttgttcgtga actacgcttt ttctcaatcg gactacgcgc agaataactt cgtggcgggc
     3061 gtgccaatcg gcatcgagta tcaaagacac agcacccaag tcgggctggc acatcggttt
     3121 aacaagaggg tttcggctaa attacaatac cggtacgatt attacgacga accgaccagc
     3181 ggcggagctg ccaattaccg ggccaacgcc gctttttgca tttttacgtt ccggtttcga
     3241 tgattcagag aatcaatttc agagcctccc gtgggaaaat agaattaaga aagcaggaaa
     3301 ccaggaaaag acaatctttc ttcaagaatt ttgcctgatt tcatgctttc ctaataaaaa
     3361 ctcttatctc atattctgcc gtacatctgg tcttaccgat aaaatcgcag atcctgactg
     3421 acttgacgtc aattatggga tgctactgga tcaatttaag tgattgaaat ctttacagtt
     3481 ggattcaagt gtgcatagat tcctgtcgga atggtttaca gaattggaag tttttttgtt
     3541 ccgaactgct acggaaaatg gccgcgtatt taccagaaat atccagaaaa cttgttaaag
     3601 accgatcttt ttaaatgtgc gtccagaaat ttagtatcaa ctgacccaat tttggaggaa
     3661 cctgtcggta gattttacaa aaatattact tttcggacct cctgaaatca atcacaacct
     3721 ctttttgaat tggagtaaat ggctggttat aaatgcatta catcatattt tcaaaataat
     3781 tctcaaaagt ggcaatcaat ctgctttagt attttcagcg gaatacatga ttccgagtgg
     3841 gtacggtcgg tgtcagcctt gaaatggctg atacggcgag aaaaatcaat tttatccgat
     3901 ctttgttggt tgagttggtc ggagagtgaa ggggattgct gttgacattg tggcggcagg
     3961 atttgtggct gccgccacaa tgatcaacga agaattccca tgaaatgagc atttttcttt
     4021 aattttcttc aaatttcttt ttttcctagt ggttcctttg taatggaatc cattacaagg
     4081 cgtcgtatct tcctttacaa aaaaatgagg gcgtggcacg acagacaact tcctcttttt
     4141 ctggcatacg attggcagat tttggtcggt gtaatcagaa aggcaatcta tcggactccc
     4201 gtgctgtgta cgcagtcgag gcgggacgta tgttgttggc tttctgggcc gatgcaagca
     4261 gttcctcctc ggaaaagttt tcctttggag gctgcttgga cgagaagttg cttgattcct
     4321 aagtttaaga atgcaaatga ctcagccagc ttggtattgc gcgcggacga agccgaaaca
     4381 cgaacacatt gcggcggcga atctgcgcaa acacttgggt ttggaagtct ttaatcccca
     4441 attacgcgtg gaacggtcca cgcgccgggg cttggtgcga acggttgagc cgctttttcc
     4501 ctgctacatc tttgtacgtt gcgccatcca ggaacggctg gatgacattc gttacaccaa
     4561 tggcatcagc tcgatagtgc attttgcgga cagaattccg gcagtttcag attcggtgat
     4621 cgcggaattg aaatcttgtt tcaattccgg tgaaccgatg ccggttgaag agaagctttc
     4681 acctggagcg gaagtggtgc tgggtgaagg cgctttctcg ggaatgaagg cttcagtgct
     4741 gcgagtcatg ccggcaggac ggcgtgtcca ggtgttgctg gacattctgg gccggccaac
     4801 cgctgtggaa gtggatcggc ggtttgtggc gctggagaag aacacgatgg cggacttggt
     4861 gccccttttg gcggcaccgc cgcgacccgt gttcgtgcgg gcgtagttgg atgttgtgtg
     4921 atcatcagtt cccggacttg ctgtacggag cccgggggag cagcagttgg tctttgggga
     4981 aaaattaccc ggcccaacaa gtgcggagct ttgccgaatt cggcgaggat ttaatccgct
     5041 aacagagagg ttcgatacca ccatgcgtac tcaagcaccc gaagatgccg attttttggc
     5101 gatggaacat ggttttcttc agttggcgga ggccatcggc tctgtcctca agatgactga
     5161 tgcggaagat ggccggacgc tctatatcag cccgggattt gaggagattt ggggacgctc
     5221 ttgcgaagat gtgcgcaact caacgcgcgc ctggtacgac accatccatc ccgaggaccg
     5281 cgaccggatg ctcgacgcgg gtttggggat ccgcaggcac ggcggcttcg acgaggaata
     5341 tcgcatcatc cggcccgacg gctcaatgcg atgggtgcgt gaacgcgcat ttggcatacg
     5401 caatgcggcg gggaaagtat cgcaggctgc gtccatcacg gaggacatca cggagcgcaa
     5461 gcttttggag aaagaagtca tcgagaataa cgaccgcgaa cgcgcccaat tgggccggga
     5521 tttgcacgac ggaatttgtc agcaactggt gagcatcgcg tttgcgacag atctgttgcg
     5581 gcgcgattta gcggcacaat tgcccggtga agcggtgcga gcgggcaagg tcacgtcatt
     5641 gctggacaat gcgatcaccc aagcacggaa tttgtcgcac gcattgtgtc ctgtgaactt
     5701 ggcgggagac gggcttgctg tcgcgttgcg cagcctggct ggcgccgtca acaaaggttt
     5761 tggcgtggtt tgcggcgcgg attgttccga gaaagttttc atccacgatc acgccgtcgc
     5821 aacgcacctt tatcgcatcg cgcaagaagc cgtgcaaaat gcagtccaac attcccatcc
     5881 cacgcagatg cttatccggt tgacgcagga aggcgagaca gtgcgcttga gcgtgactga
     5941 caacggaaaa ggcgctgatg aagaaggcga acgcgttttt gcagttggat tgaacatcat
     6001 caaattccgc acacagatga cgggcgggcg gttgaaggtc gagcgaagcg ccgcgggcgg
     6061 cacgacgatt tcgtgcatgt tccagcaaaa gccgccagga cagcgcaaga cggaaacaga
     6121 ttttcaactg ctccatgcaa aagcgtgaat gggaatcgtt tcacgaacgt cactacctgc
     6181 tcaatttgct gtaacatcat gaccataatt tcagtctgat aacttttacc cgcaagaaaa
     6241 tggaatctct cgctgacatc aaaagcaagg acccggcgcc gatcgcgccc ggcccgcgcg
     6301 aggtgccgcg ctggaaacgg gtgttggaca ccgcgttgat cctgctggcc atgccgttgt
     6361 tgctgccgtt gatgctgttc atcgccgtgc tggtgcgttg cgtgtcggcg ggcccggtat
     6421 tattccggca ggagcgtgtg gggcatctgg gcagccggtt ccgctgtttc aaattccgca
     6481 caatgcaagt cggcaacaac gccgcggtgc accagggcca cttgaaagag ttgatggatt
     6541 caaacgcgcc aatggtgaag atggatgtga aaggcgacgc gcgcatcatt ccgtttggtg
     6601 ttccgttgag ggcctcggga ttggatgaat tgccgcagat tttcaatgtg ctattcgggc
     6661 agatgagcct ggtcgggccg cgtccctgcg tgccctacga atatgacagc tatctgccat
     6721 ggcaaaagga gcgcttcgac gttctgcccg gcctgaccgg tttctggcag gtgagcggca
     6781 aaaacaacac cacgtttgaa gagatgatgc ggctggacat ctattacgcg cgcaatagtt
     6841 cggttggatt cgacttgcgc atcattttca agaccgtccc cgcgctcctc atccaaatgt
     6901 ttgaaacacg ccggcggaga aaggcagaac aacgcgccac tttgaatggc ggtggtggtg
     6961 gcgcggcgac cagcccggct ttgctgaggt caggaaccaa tctgcccacg acacaattta
     7021 aaatgactgc acgaaccaag aatcaggagg cattatgacc aaacaaatca aaatcggagt
     7081 tgtaggattg ggctattggg gcccgaatct ggtgcgaaat ttcagggcgc tgccggattg
     7141 cgatcttcgg atgatgtgcg acttgagcga agagcgcctc gatcatctca catcgctcta
     7201 tcccgaagtg gaggcggcat cggatttcaa gcacatgttg aacggcgcga acttggatgc
     7261 ggtggtgata gcgactgccg tgcgttcgca ttatccaatg gcaaaggcat cattgctggc
     7321 gggcaagcac acgttcatcg agaagccgat ggcttcatct tccgaacaat gcgaagaatt
     7381 gattgacatc gcgcggcgca agggactggt gctgatgacc gggcacacgt ttctctattc
     7441 gcccgcggtg cggaaaatca aggaactcgt ggacagcggg gacatcggcg aaatccggta
     7501 catttctgcg cgccggttga acctgggctt gttccaaaag gacatcaacg tggcgtggga
     7561 ccttgcgcca cacgacattt ccatcatcca gcacatcatc ggcgaacaac cggtgacggt
     7621 gaattgccgc ggcagcgcgc acatcacgcc cggcatcgag gacgtgacca cgatgtgcct
     7681 gagcttcgag aagcagcgca cggccatcat ccacagcagt tggctggatc cgcgcaaggt
     7741 ccgggaaatg acgattgttg gcagcaaacg gatgattgtt tatgacgatg tgatgcagca
     7801 ggagaagatc aagattttcg acgcgcgcgt cgagcgtccg ccgcattacg acacgttcgc
     7861 cgaattccat tacgcttatc attacggcga cgtttatgcg ccgtatctca agcaggagga
     7921 accgctgaag attgaatgcc agcatttcct ggattgcatc aagaacggct ctgccccatt
     7981 gacgagcggt gaacgcggat tggatttggt gcgcatattg gaagcgtcgt ctgagtcgct
     8041 caagcttggc ggtggcccgg ttgaactggc cccgagcctg gccgtgaagg ttgcattcaa
     8101 ccctgccgct gtgcgcaacg gcaatggtca tcgaaatggg aatgggaatg gaaatggcaa
     8161 cggccatgca gtggcagcca aaaaacgcgg cgcgcgtccg actgctccca aagttgcggt
     8221 gaatgggcgg tgagcgagta accttgtaac catttaacga acccatgtcg catcaagttt
     8281 ttcaacgcat cgcccccgat gtgaagctgg gaaaaaacgt tcgcatttac gatttcacga
     8341 acctttacgg atgtgaaatt ggcgatgacg tgaagatagg cacgtttgtg gaaatccaaa
     8401 ggggcgcgaa gataggcaat gcctgca
//
LOCUS       DASZMM010000200         8077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4743096, whole genome shotgun sequence.
ACCESSION   DASZMM010000200 DASZMM010000000
VERSION     DASZMM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4743096"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VG754_04780"
     CDS             <1..789
                     /locus_tag="VG754_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWF18555.1"
                     /translation="LVHNLPLTEANHFLPDLDSIPKEILSKAKVLVLNYPNNPTGASA
                     TPEFFARVVDFARKHRIVVIHDAAYAALVFEGKPLSFLATPGAKEVGIELHSTSKTFN
                     MTGWRCGFVAGNELLVKAYGDIKDNTDSGQFLAIQHAAAYCFVHPEMTEKIAAKYSRR
                     MDGLVKVLRDAGFNARKPRGSFFLYVPAPKAAQPRSGARVEFKNAEEVSQWLISEKLI
                     STVPWDDACANLRFSVTFAAKDPTDEKRVLAEIATRLNDVQFVF"
     gene            971..1522
                     /locus_tag="VG754_04785"
     CDS             971..1522
                     /locus_tag="VG754_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HWF18556.1"
                     /translation="MNQTINREDGVAVRTRPVKSAHAIHHHAPIGLRTVAVFEFAKGL
                     IVLVAGLGLLSLIHRDAQAVAEDIVMRLHMNPAHFYPRIFIQFASNLTDARLWFMSLG
                     ALAYAGVRFIETFGLWHERAWAEWFAVISAGLYLPVELWHLYDKPGPVSFSLPLINIG
                     IIIYLARLLAINARRRQNCGTDS"
     gene            1555..2817
                     /locus_tag="VG754_04790"
     CDS             1555..2817
                     /locus_tag="VG754_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWF18557.1"
                     /translation="MNHSDLVLLAVSILLVGKWAAQLWLAHLNRRNVLANANTIPDAF
                     KDSVDADTYSKSVQYTLAKNRFGNIEMTWDLLVLAAVLFSGLLPWMFRLFSGWLGTSA
                     WAMAAYLFAIGILLALPDIPFEWYSQFHLEEHFGFNTNTPKLWLVDRLKALLLALVLG
                     YPLLVLVLKLVDWTGPFWWLWAWAALLVFQLLMFVLAPVLIMPIFNKFTPLPEGSLRE
                     RLLALGQRTGFKSRSIQVMDGSKRSRHSNAFFTGFGQFRKIVLFDTLIQQLAEPELEA
                     VLAHEIGHYKKKHIPKMLLWSAATSLVAFYLISQLAKQDWFYRSFGFAPGSIAPALLL
                     FGLLAGVVTFWFSPLAHWWSRRHEYQADAYAAETMREPQSLIGALRKLNEKNLSNLTP
                     HPFYSGFYYSHPTLLEREQALRGHFHAA"
     gene            2879..3808
                     /locus_tag="VG754_04795"
     CDS             2879..3808
                     /locus_tag="VG754_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF18558.1"
                     /translation="MMKASPSVSRIRSWALGLLLFGSLGHIAFAQSHATEPDYSFFVA
                     GDMRSFLWHAPEGKMYFDGACDAMKQAGPAAFLLSPGDCDPPGSVREIIDLHLGTNFL
                     WYPLVGNHDVEAASNMDWWRKWAKAGIPHLVRSGPEGNEALTYSFDYGNSHIIMLDEY
                     FNGHSSSPTDDMSESTIVWLEHDLAANHQPIVWVSGHKPIKSLPDMDTGRIRHQDQSI
                     ITDPDRRKRFLSLLRKNHVTGYICGHTHDCSIAKVDGIWQLDSGHCRGAGDNACPSTF
                     LKIQVTGLKATVDVYRSNTNGLNYLIKKTVELN"
     gene            complement(3856..4284)
                     /locus_tag="VG754_04800"
     CDS             complement(3856..4284)
                     /locus_tag="VG754_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWF18559.1"
                     /translation="MRFFIRKRRQPPAVIIVALIDVLIVVLIFLMVTTTFKKPQPSLK
                     LALPESSHSTKTGASEAPPLVVSIEENGVLHFGPDAKPYTPDDLKKELIAASTKNPQL
                     KLTLRADTKAPFGRIVKVMDIAKEANIKMVDAFTKPSAPQ"
     gene            complement(4287..4904)
                     /locus_tag="VG754_04805"
     CDS             complement(4287..4904)
                     /locus_tag="VG754_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWF18560.1"
                     /translation="MELFFKIIMLVTSMVGLAFIIERGLALRWNRVLPRSIEDAAEAC
                     RTPADRPVLRRLCEQSPSALARLLLAAEEHLDLPKEENESAVQTRARQEIVRLERGLV
                     VLEIVVGIAPLLGLVGTIYGMMALFGSLGESGLGDNTVLAKGISLILQFTMMGLLIAI
                     PSLIAWNYYSKKVEMLAVEMENVCAEFLRRQYQAQVQPRKVAATK"
     gene            complement(4966..6000)
                     /locus_tag="VG754_04810"
     CDS             complement(4966..6000)
                     /locus_tag="VG754_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWF18561.1"
                     /translation="MSVSRLNEQTLRPAEHFLAWAFGISLGIHVLLYGGFNLGNHYGW
                     WKKDLMPSWLKSTKQALAEIKKAQQKQPVNQAQQEPPMLFVEVDPAVATQEAPKNATH
                     YSSHNSHAGNPDITIDSNDPNINGKQTHVAKTQDADRSKPVPLRPSPPKTPPSPDESA
                     QEAKAKPNGGPKPGDLAMAKPAQQPGDVQINPDTGEAKAPTHKRPHTLLEALQQQPSA
                     AIPGQKMKQEGGVQHHLDSSAYDAIATPFGEYDRELIDAITTHWWSLLDRKEFSQDHT
                     GRVIVQFTLRSDGRVTDIHVVHSDVGDLLASVCELAITDPAPYAPWPGDMRRYYAKDS
                     LDITFTFWYE"
     gene            complement(6073..7563)
                     /locus_tag="VG754_04815"
     CDS             complement(6073..7563)
                     /locus_tag="VG754_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF18562.1"
                     /translation="MFPEFTPHPLFKEYVRQETRRQFFKRGASFMGAAALGMLAPQLL
                     RGAEKNATSSASSLGAAGPNFAPKAKQVIYLHMVGGPSQMDLYDYKPVMGDWYDKDLP
                     ETIRMGQRLTTMTSGQARFPIAPSKYKFERHGKNGMWVSELLPWTAKMVDDMCFVRSM
                     HTEAINHEPAICFMQTGNQITGRPCLGSWVSYGLGSLNQDLPTFVVLVATPTNQEQVQ
                     AISGRLWSSGYLPGKHGGVSFRSAGDPILYINNPSGVDANVRRKMLDGLKALNEINFR
                     EVGDPETHTRIDQFELAFRMQASVPELTNVANEPASTYELYGDEAKKPGTFANSALMA
                     RRMVERGVRFVQIYHNNWDTHANVSGRLPMQCKDVDQACYGLVQDLKQRGLYDSTLVV
                     WGGEFGRTIYSQGGLSKDNYGRDHHPRCFTMWMAGGGSKPGTIYGETDDFSYNIIKDP
                     VHIHDFHATVLTLLGLDHERFTFRYQGLDQRLTGVEPARIIRELIA"
     gene            complement(7568..>8077)
                     /locus_tag="VG754_04820"
     CDS             complement(7568..>8077)
                     /locus_tag="VG754_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HWF18563.1"
                     /translation="AMTASNTRFYKRDSGDKLYRRSLYTFWKRAAPPASMEIFNAPSR
                     ETCTVRRERGDTPLQALVTMDDPQFVEAARHLAQLALEQGGKSDEERIDFMAECLLAR
                     PLRSDELKITRSVFKDLLAHYQAEPKSAEALLAVGESKADPKLDKSSLAAYAMVANEL
                     LNLDEVLNK"
BASE COUNT         1747 a         2169 c         2208 g         1953 t
ORIGIN      
        1 cttgtccaca atcttccgct caccgaagcc aatcattttc ttccagacct cgacagcatc
       61 ccgaaagaaa ttttgtcgaa ggcgaaagtt ctggtcctga attatccgaa caacccgacc
      121 ggcgcaagcg ccacgccgga atttttcgcg cgcgttgtgg atttcgcccg taagcatcgc
      181 atcgtggtga ttcacgacgc cgcgtatgcg gctctggttt tcgaaggaaa accgttgagc
      241 tttcttgcca cgccgggcgc aaaagaagtc ggcatcgaat tgcattcgac aagcaaaact
      301 ttcaatatga ccggctggcg ctgcggattt gtcgccggga acgaattgct ggtgaaagct
      361 tacggagaca tcaaggacaa cacggattct ggacagttcc ttgccatcca acacgcggcc
      421 gcgtattgct ttgttcaccc tgaaatgaca gaaaaaattg ccgccaaata ttcgcgccgg
      481 atggacgggt tggtgaaagt gttgcgcgac gcgggcttca acgcgcgcaa gccgaggggt
      541 tcattctttc tttacgttcc cgcgccgaag gctgcgcaac cgcgtagcgg cgcgcgcgtc
      601 gagttcaaga atgccgagga ggtttcccaa tggctcatca gcgagaaatt gatttccacg
      661 gtgccgtggg acgacgcttg cgccaacctc cggttcagcg taacgtttgc ggcaaaagac
      721 cccactgatg aaaagcgcgt gctcgcggaa atcgcaacac gcctcaacga tgtgcaattc
      781 gttttttagt ataaagttat gcagaatgtg aggcaaatga cggtccagac aatgctttac
      841 aaaatgcgtt atatggccca tgttacggat gtcgatgaac cgtcattttc agtcgtcagg
      901 tcacaaaact ttaaaaggtg gtcgcagcgc attttctgaa ttgttgcaaa atcgtcgctg
      961 ccactaatcc gtgaaccaga ctataaatcg cgaggacggt gtcgccgtca gaaccagacc
     1021 ggtgaaatcc gcacacgcca ttcatcatca tgcgccgata ggactgcgca cagtggccgt
     1081 cttcgagttc gcaaagggtt tgattgtgtt ggtcgccggg cttggcttgc tttccctcat
     1141 tcaccgcgac gcgcaggccg ttgccgaaga cattgtcatg cgtttgcaca tgaaccccgc
     1201 gcatttctat ccgcgaattt tcatccaatt tgcttcaaat ctcactgacg cgcggctctg
     1261 gttcatgtca ttgggcgcat tggcgtatgc gggggtgcgg ttcatcgaaa cattcggcct
     1321 ttggcacgaa cgcgcctggg cggaatggtt cgcggtgatt tccgccggac tttatctgcc
     1381 ggttgagctg tggcatctgt atgacaaacc gggcccggtg agcttttcac tgcccctcat
     1441 caacatcggc atcatcatct atctcgcacg gctccttgcc atcaacgccc ggcgcagaca
     1501 aaattgtggc acagattctt agcccgccat cagcaggcaa agacttctca gacaatgaac
     1561 cattctgacc tcgttcttct cgcggtgtct attttgctcg ttggcaaatg ggcggcgcag
     1621 ctttggctcg cgcatctcaa tcgccgaaac gtcctcgcga acgcgaatac cattcccgat
     1681 gcattcaagg attccgtcga tgctgacacg tattcaaaat cagtccaata cacgttggca
     1741 aaaaaccgtt tcggcaacat cgagatgaca tgggatttgc tcgtgcttgc agcggtgctt
     1801 tttagcggat tgctgccgtg gatgtttcgg ctgttctccg gctggttggg aacatcggca
     1861 tgggcgatgg cggcgtatct tttcgccatc ggcattttgc tcgcgctgcc ggacattcct
     1921 tttgaatggt actcgcagtt ccatctggag gaacatttcg gtttcaacac caacaccccg
     1981 aagctttggt tggtggacag gctcaaggca ttgttgctcg cgctcgtgct tggatatccg
     2041 ttgctcgtgc tggttttgaa gctggtggat tggacgggac cgttctggtg gttgtgggca
     2101 tgggcggcgc tgctggtatt tcagttgctg atgttcgtgc tcgcgccggt tttgatcatg
     2161 ccgattttca acaaattcac gccgttgccg gaaggcagcc tacgcgaacg actgctcgca
     2221 ctcgggcaac gcaccggttt caaatcgcgc agcatccagg tgatggacgg cagcaaacgc
     2281 tcgcgccatt cgaacgcgtt tttcaccggg tttggacagt tccgcaaaat tgtgctgttc
     2341 gacacactca tccagcagct cgccgaaccg gaattggagg ccgtgcttgc gcatgaaata
     2401 ggccattaca aaaagaagca catccccaag atgttgctct ggtccgccgc aacttctctg
     2461 gttgcatttt atttgatttc gcagcttgcg aagcaggatt ggttctatcg cagctttgga
     2521 tttgcgcccg gcagcattgc gccggcgctg ttgctgttcg gtttgctcgc gggcgtggtg
     2581 actttctggt tttcgccgct ggcgcattgg tggtcgcgac gccacgaata tcaggcggac
     2641 gcttacgccg cggaaacgat gcgcgaaccg caatctctca tcggcgcgtt gcgcaagctc
     2701 aacgaaaaaa atctcagcaa tctcactcca cacccgtttt acagcgggtt ctattattcg
     2761 catccgacgc ttcttgaacg cgaacaagcc ttgcgcggcc attttcatgc cgcatgactg
     2821 atgaaaaaaa tgattttcca tttgtcgcac ctgccatatt agtcaaaact gctctcctat
     2881 gatgaaagcc agtccttcag tttcgcgcat ccgttcttgg gcgcttggtt tgcttctgtt
     2941 tggttcattg ggccatatcg cattcgcgca gagccacgca accgaacccg attattcgtt
     3001 ctttgtcgcc ggtgacatgc ggagttttct gtggcatgcg ccggaaggaa aaatgtattt
     3061 tgacggcgcc tgcgacgcga tgaaacaagc cggcccagcg gcatttctgt tgagcccagg
     3121 agattgcgat cctcccggct cagtgcgcga aatcatcgac cttcatcttg gcacaaattt
     3181 tttgtggtat ccactggttg gaaatcatga cgtcgaggcc gcgagcaaca tggattggtg
     3241 gcgtaaatgg gcgaaggctg gcattcccca tttggtgcga tccggtccgg aaggaaacga
     3301 ggcgctgact tactcgttcg attacggaaa ttcgcacatc atcatgttgg atgaatattt
     3361 caacggccat tccagttcgc cgacagatga catgtccgaa tccacgattg tctggttgga
     3421 gcatgacctt gcagcgaacc atcaaccgat cgtgtgggtg agcggccaca agcccatcaa
     3481 atcgttgcca gacatggaca cgggccggat tcggcatcag gaccagtcta tcatcacaga
     3541 cccggaccgg cgcaaacgtt tcctgtcttt gcttcgaaaa aaccatgtca caggttacat
     3601 ttgcggccac acgcatgact gttccatcgc aaaagttgac ggcatctggc aattggactc
     3661 gggccattgt cgcggggccg gagacaacgc gtgtccaagc acttttttga aaattcaagt
     3721 aactggattg aaagcgacag tcgatgtcta tcgctcgaat acgaatgggc tgaattacct
     3781 gatcaagaag accgtggagt taaactgagt ttccaatgaa tttgagattt caaattttcg
     3841 aatttaaaat tcgattcact gcggggctga aggcttggtg aacgcgtcca ccatttttat
     3901 gttcgcttct ttggcgatat ccatgacctt gacgatgcga ccgaaaggag ctttggtgtc
     3961 cgcgcgaagg gtgagtttaa gttgtggatt tttcgtggat gccgcaatca attccttctt
     4021 gagatcgtcg ggggtgtagg gtttggcatc cggtccaaaa tgcaacacgc cgttttcttc
     4081 gatgctcacg acgagcggcg gagcttcgct tgcgccggtc ttggtcgaat ggctggattc
     4141 aggcaaggca agtttcaacg acggctgcgg tttcttgaat gtcgtcgtga ccatcaaaaa
     4201 aatcaacacg acgatgagca cgtcaatgag cgccacgatg atgaccgcgg gcggctggcg
     4261 tcttttgcga atgaaaaagc gcatggttac tttgtggcgg caactttccg cggttgcacc
     4321 tgcgcctggt attggcgccg gagaaattcc gcgcagacat tttccatttc cacagcaagc
     4381 atctcgacct tcttgctgta atagttccat gcgatcagcg aaggaatggc gatgagcagg
     4441 cccatcatcg tgaattgcag gatgagcgaa atgccctttg cgagcacggt attgtcgccc
     4501 aaaccggatt cgccgaggct gccaaataac gccatcattc cgtatatggt gccgacaagt
     4561 ccgagcagcg gcgcgatgcc gaccacaatt tcgagaacca caaggccgcg ttcgagccgg
     4621 acaatttcct ggcgagcgcg cgtttgcacc gcgctttcgt tctcttcttt tggcaggtcg
     4681 agatgttctt ctgccgcaag caaaagccgc gcaagcgccg acggagattg ttcgcacaag
     4741 cgccgtaaca cgggacggtc cgcaggtgtc cggcaggctt cggcggcgtc ctcgatgctg
     4801 cgcggcagca cgcggttcca tcgcaatgcc agaccgcgtt cgatgatgaa tgccaagccg
     4861 accattgagg tcacgagcat gatgatttta aagaatagtt ccatcttgtt ggtggtaggt
     4921 tgtgaatcag caaaagtttt tcagatgatg aatttgaaat ccgttttatt cgtaccaaaa
     4981 tgtgaatgtg atatcgaggc tgtctttcgc atagtagcgg cgcatgtcgc ccggccacgg
     5041 agcataaggc gcgggatcgg tgatggccag ttcgcaaaca gaagccagaa ggtcgcccac
     5101 gtcactgtgg acgacatgga tgtctgtgac acgaccgtca gaacgcaaag tgaactgtac
     5161 aatgacccgt ccagtgtggt cctgagagaa ttccttcctg tcgagcaaac tccaccaatg
     5221 cgtcgtgatc gcgtcaatca actcccgatc atactcgcca aacggtgtcg caatcgcatc
     5281 atacgctgat gaatccagat gatgttgcac accgccttcc tgtttcatct tctggcccgg
     5341 aatggccgcg gacggctgtt gttgcaaggc ttctagaagc gtgtgtggcc gtttgtgcgt
     5401 cggagccttt gcttcgccgg tatccgggtt gatttgaaca tctcccggtt gttgcgcagg
     5461 tttggccatg gccagatcgc cgggtttggg accgccgttc ggcttcgctt ttgcttcctg
     5521 agcggattcg tcaggagaag gcggcgtctt gggaggagaa ggccgcaacg gaacgggttt
     5581 ggaacggtca gcatcctgcg tcttggcgac atgcgtttgc ttgccgttga tattgggatc
     5641 gttgctatca atcgtgatgt ccggattgcc cgcatgtgaa ttgtgggagg aataatgggt
     5701 cgcattcttc ggcgcctcct gcgttgcaac agctggatcc acttcgacaa acagcatcgg
     5761 cggctcttgt tgcgcctgat ttacgggctg cttctgttgt gctttcttga tttcagcgag
     5821 agcttgtttg gttgatttca gccaggaagg catcaggtcc tttttccacc agccgtagtg
     5881 attccccaga ttgaatcctc cgtagagcag cacatggatg ccgagcgaaa tgccaaaagc
     5941 ccacgccaga aaatgttcgg cggggcgcag cgtttgttca ttcagcctgc ttacagacat
     6001 accgcacaga atgccgtaga aagaatggac gggcaactgt ttagcacggt attaaatgca
     6061 ttcagcctca actcacgcga tcagttcacg aatgatgcgc gcgggttcga cgccggtgag
     6121 gcgttgatca agtccttgat agcggaaggt gaaacgctcg tggtccaagc cgagcagggt
     6181 gagcacggtc gcgtggaaat cgtggatatg gacagggtct ttgatgatat tgtaagaaaa
     6241 atcatccgtt tcaccgtaaa tggtgcccgg tttggagccg ccgcctgcca tccacatcgt
     6301 gaaacagcgc ggatgatggt cgcgtccgta attgtccttc gagaggccgc cttgtgaata
     6361 aatcgtgcgg ccgaattcac cgccccagac gaccagcgtt gaatcgtaca atccccgctg
     6421 tttgaggtcc tggaccaatc catagcaagc ttgatcaacg tccttgcatt gcattggcag
     6481 gcgtccggaa acattcgcgt gagtgtccca attattgtga taaatctgca cgaaccgcac
     6541 gccgcgctca accatccgcc gggccatcaa cgcgctgttt gcgaaagtgc cgggcttctt
     6601 cgcttcatcg ccgtataact cgtacgtgga tgcgggctcg tttgcgacgt tggtcaattc
     6661 cggcacactc gcctgcatcc tgaaagccaa ctcgaattga tcaatgcgcg tgtgcgtttc
     6721 cggatcgccc acttcgcgaa aattgatttc gttgagcgcc ttgaggccgt cgagcatctt
     6781 gcggcggacg ttcgcgtcca cgcccgaagg gttgttgatg taaagaatcg gatcacccgc
     6841 cgatcgaaac gaaacgccgc catgcttccc cggcagataa ccggaagacc aaagccgccc
     6901 ggaaatcgct tggacctgct cctggttcgt tggcgtggcc acaaggacaa cgaaagtcgg
     6961 caaatcctga ttcagcgaac caagcccata cgaaacccac gagccaagac aaggacggcc
     7021 tgtgatctgg ttgccggtct gcatgaaaca aatggccggt tcgtgattaa ttgcctcggt
     7081 gtgcatcgaa cggacgaagc acatatcgtc caccatcttt gccgtccatg gcagcaattc
     7141 gctgacccac atgccatttt ttccatgccg ctcaaacttg tatttcgatg gcgcaatcgg
     7201 aaaacgcgcc tgcccggatg tcatcgtggt gagccgctgc cccatgcgaa tggtttcggg
     7261 caggtctttg tcataccaat cgcccatgac cggtttgtaa tcatacaggt ccatttgcga
     7321 cgggccaccc accatgtgaa gataaatcac ctgtttggcc ttgggcgcaa aattcgggcc
     7381 ggcggcgcca agactcgacg ccgaactggt cgcatttttt tccgcgccgc gcaggagttg
     7441 cggggcgagc attcccaatg cagccgcccc catgaaactc gccccgcgct tgaaaaattg
     7501 gcggcgtgtt tcctggcgga cgtattcctt gaacaacggg tgcggagtaa attcgggaaa
     7561 catagggtta tttgttcaac acttcgtcga gattcaagag ttcgttggcg accatcgcgt
     7621 aagccgccag agacgacttg tcgagcttgg gatctgcttt ggactcgccg accgcgagca
     7681 atgcctcggc agatttgggc tcggcttgat aatgcgccag caaatcctta aataccgacc
     7741 gggtgatctt cagttcatct gaacgcaacg gacgcgccag caaacattcc gccatgaaat
     7801 caatgcgctc ctcatcgctc ttgcctcctt gttcaagcgc gagttgcgcc agatgccgcg
     7861 cagcctcaac aaactgcgga tcgtccatgg tcacgagcgc ctgcaacggc gtgtcgccgc
     7921 gttcgcgccg cacggtgcaa gtctcgcggc ttggtgcgtt aaatatttcc atcgatgccg
     7981 gtggcgcggc tcgcttccaa aaagtgtaga ggcttcggcg atacaattta tcgccggaat
     8041 cgcgtttgta gaaacgcgtg ttgctggcag tcatggc
//
LOCUS       DASZMM010000201         8098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3098455, whole genome shotgun sequence.
ACCESSION   DASZMM010000201 DASZMM010000000
VERSION     DASZMM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3098455"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="VG754_04825"
     CDS             complement(<1..1850)
                     /locus_tag="VG754_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18564.1"
                     /translation="MAAQENLVKNPGFEEIGAANNMPAGGWWPFEGRGSATFTVDSNT
                     AHSGKSSLRLTGDAEAKFTFVSPKFEVAPGDELHFETWLRAQNLPTNQSTISAGLAFR
                     HRDGRVVQRDYFHLEKPGENWSLISGTARAPAEAVSAEVHLGYANGPGTIWYDDVVAT
                     ITNPVSLALVEGAKSWPGAQEITVKISNRQTKEFRGTITCAIAGKVSQMPAVIAPQSS
                     RTFKLPITLNGMGQHHYRLSLLDAAGNSLRLLEGNFPTTAPLSLFPACPCYHVAGEGD
                     GTTRIDARVNMNPAQRDGLKLVLEVLDSAGKPVQKMEANASKGEFVGTILKVPNDQSA
                     VFTIAASLQDHAGKEVAADKTEVHVAATDDSRVILGHDGFLRIGGQPHFPIGIYNCGR
                     FEEMGKAGFSATHEYGITTGEAEDPINPNERRVQQLLDESWSNGMRVMIELPRKAIEK
                     ARWDQVRHRIETFRHHPGLLCWGSEERVARGIAPLANIAELYALVHELDPEHPLVLGD
                     TRDVIQNLQVDRRNFFPDPDMDIGIWWWYPIPLKEPDGNGLEGGSDKANLLEPPSWLT
                     TTLSKKPLWIAVQSYQKPAKDAHFPTPAEYRAQAYLSIINGVKGLFFYVG"
     gene            complement(2005..2643)
                     /locus_tag="VG754_04830"
     CDS             complement(2005..2643)
                     /locus_tag="VG754_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF18565.1"
                     /translation="MTKIAIVEDSKVIRESLAEFVHLDQENRCVCACSTAEEALVSIP
                     KFAPDIVLMDIQLPNLSGIECTARLKQMLPSLRIIVVTVYEDTDRIFNALRAGACGYL
                     LKRYSPDEIVSAIREVRQGGAPMSREIARKVIASFQEPLTRAIEVEDLTRREREILEL
                     LAEGFSNKEIAERCGLSGGTVRWHLQHVYEKLHVRTRTEAALKFRSTKPDDN"
     gene            complement(2640..5651)
                     /locus_tag="VG754_04835"
     CDS             complement(2640..5651)
                     /locus_tag="VG754_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18566.1"
                     /translation="MTNTLQLRRLASQQGIVSCSIRLEGVILWVSSTQNQLAIQDDSG
                     GFILKVDLHSLETPAKPGQRVLIEGTCRAGQGEAGSSIAVVNNDGTHALYEQSGKIYL
                     SAGFHPIRVEWFNNVGKFALKIDCQCPGMKRESIPDAALFLRDNKTGQLTNGLVYQVF
                     EGAWSWLPDFSRLPVSKSGTISNFDIGIRTRDTNVGVVFTGYFEALRDGVYHFWTTSD
                     DGSKLYVGDSPLGVRVLGNTAMPFPEPVIPGQLVQETQEGRWSEMEGMVSVIRGPSIS
                     PNLEINSGTGRAYLKVMECGPESLDFLLHHRVKITGIYQSALSVDGQKVPSLLVPNKD
                     DIVILDPQPIQSSLEASNGLPLLTTALQVMNLGRKEALRGYPVRLQGVVTAHVDVNFF
                     IQDSTWSIYVGDLNTFSGGRLPRIGECWQIEGISSADFAPDIKVKHAVYLGPGILPAP
                     IRPTWDELINGSLSTKYMEIQGVVSQIRSNEFVLLTRGGKITILCVDLENDELKGLQD
                     ALIHISGVGSSDRDEHQMMRQRLRIYNASVKVDEPAPADPFSAPLKKANDLFFFDARA
                     DTLRRVKISGQVMREYHGEFFLMDGTHGFRFRPKAALQLPVGTFVEVVGFPDRNGPSP
                     VLREGVARRTGKADLPIPKRLVEQNMLNPDLDATLVRIKSRLIGSALEGAEQTLELQL
                     GNRSYFARLQRGGGLVTKLEPGCELELTGVYIGQRVDRTVSRDIDSFELLLNSPADIQ
                     VLTRPSWWTIRHAVMSMTAMLSVLAAAFVWITSLRRQVEERSLQLTAEIKSRERAERQ
                     RALEEERARIAQDLHDDLGSGITEMGMLVALMKSTCTPDEKRNRYLEQVHEKSQEMVT
                     ALDEIVWAMNPRHNVLGSLVSYFSLYADRFLGLAKISWRLEKPLGAPDRVVDSRHRYQ
                     LFLAFKEALTNIVRHSHASEVRISIHAEEEHLRLTIVDNGSGFSAEMHTDEMNGVINM
                     RRRVEKLGGKFELISEIGSGTTLRFILPLSS"
     gene            6263..7822
                     /locus_tag="VG754_04840"
     CDS             6263..7822
                     /locus_tag="VG754_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18567.1"
                     /translation="MNSYFNAADWWVIFLYLGGIILLGVWFGKDQKNTRDYFLGSRNI
                     PWWGIGLSIVAAETSALTIIGVPSMAYGTNMAFLQMIIGYVIARVILAIVMVPHYFKG
                     EIYSPYQLFSNAFGPSVRQMAGGFFLLSETLAAGVRVYVACIPIKLMLGDKFLGFLTN
                     DPILGAILLFVLLSLLYTYIGGVKAVIWTDAVQFGLFLAGGLFTLFYIPSFFKGGAKE
                     VFHLASAGGKLHWLNGAFTLGAPINIWMGIIGGTVMVMSSHGAEQLIVQRVLACKNVA
                     DGRKALALSAVIIFPLFLIFLLCGAMLWVFYQAHPFQIPLPEARAGIKSNDFIFPIFM
                     MTEVPHVLKGFLIVAILSAAMSSVSSALTSLSSVSTMDFVKALSRKQRSEEFYLKFSK
                     ISTIAWAIMLILVAYISRRVEFVLNASFSLRGLTSGALLGGLLLAVFWKRGRATPILI
                     GMAVSLLVMIAIQVLPNYFPSIPAIFWPWYALIGTTVMVLVSWSVREMMPAHEEEQKT
                     GTPERKIGAVD"
BASE COUNT         1853 a         2165 c         2112 g         1968 t
ORIGIN      
        1 aaaccgacat agaagaacag ccctttcacc ccgttgatga tgctcaagta agcctgtgcg
       61 cggtattcag caggcgtggg aaaatgggcg tccttggccg gtttttgata cgattgaacg
      121 gcgatccaaa gcggcttttt ggagagggtt gtggtgagcc acgaaggggg ttcaagcagg
      181 tttgccttgt ccgagccgcc ttcgagacca ttgccgtcgg gttccttcag cggaatgggg
      241 taccaccacc aaatgcctat gtccatgtcg ggatcgggaa agaaattgcg gcggtccacc
      301 tgaagatttt gaatcacatc gcgcgtgtcg ccgagcacaa gtggatgttc tgggtccaac
      361 tcatgcacaa gcgcataaag ttcagcgatg ttcgccagcg gcgcgattcc gcgcgcaacg
      421 cgttcctccg aaccccagca gagcagcccc ggatgatgcc gaaacgtttc gatgcgatgc
      481 cgcacttggt cccaacgcgc cttttcaatc gctttgcgcg ggagttcaat catcacgcgc
      541 atcccgttgg accacgactc atccagcaat tgttgcacac gacgctcgtt gggattaatt
      601 gggtcttccg cttcgccggt cgtgatgccg tattcgtgcg ttgcgctgaa accagccttg
      661 cccatctcct cgaaacgacc gcaattgtat atgccaatgg gaaaatgcgg ctggccgccg
      721 atgcgcagaa aaccatcgtg ccccaagatc acacgcgaat cgtccgttgc ggccacatgg
      781 acttcggtct tgtcagcagc cacttctttt ccggcatgat cctgcaggct tgccgcaatg
      841 gtgaaaacgg cggattgatc gtttggcact ttcaaaattg tgccgacgaa ctcgcccttg
      901 gaagcgttgg cctccatttt ttgaaccggc ttgccagcag aatcaaggac ttccaaaacc
      961 aatttcaatc catcgcgctg tgctggattc atgttcaccc gcgcgtcaat tcgtgtcgtg
     1021 ccatcgccct cgcctgcgac atggtagcaa ggacatgcgg ggaacaagct caatggcgca
     1081 gtcgtcggga aatttccttc gagcaggcgc aaagaatttc ccgccgcgtc cagcagactc
     1141 agcctgtaat gatgctgccc cattccattc agcgtgatgg gaagtttaaa agtgcggctg
     1201 ctttgcggcg caattaccgc aggcatttgg gagacctttc cggcgatcgc gcacgtaatc
     1261 gtgccgcgaa attcctttgt ttgccggttg gaaattttca ccgtgatttc ctgcgcgccg
     1321 ggccaggatt tcgcgccctc gaccaaggca agcgaaaccg ggttggtgat tgtcgcgaca
     1381 acatcatcgt accaaattgt tcccggcccg ttggcataac cgagatgcac ctcggcgctc
     1441 acagcctcgg cgggagcgcg cgcagtgcca gaaatcagcg accagttttc gccaggtttt
     1501 tcgagatgaa aatagtcccg ctgaaccacg cgaccgtccc ggtgccgaaa cgccaatcct
     1561 gcggaaatcg tggactggtt ggtcggcagg ttctgcgctc gaagccacgt ttcaaaatgc
     1621 aattcatcgc cgggcgcgac ttcgaatttg gggctgacga aagtgaattt ggcctcggcg
     1681 tctcccgtga gtcgcaggga agatttgcca ctgtgggccg tgttggaatc gaccgtgaat
     1741 gtcgcgctac cacgtccttc gaacggccac caaccgccag cgggcatatt attggccgca
     1801 cctatttcct cgaagccggg attttttacg agattttcct gcgctgccaa gacttgtgcg
     1861 cagcaagcaa tcaggatgaa acaaaaacaa atcttacgca tcgcgaacga atgctggatc
     1921 aaaatctaaa tttattgaat caaaatcgtg taaatttaag ccacgtgaca atacggaacc
     1981 aaaatgagat ttgttttccg caacttaatt atcgtccggt tttgtcgagc ggaacttaag
     2041 cgcggcttcg gtgcgtgtgc gaacatgcaa tttttcgtaa acgtgctgga gatgccaacg
     2101 cactgtgccg ccgctcaagc cgcagcgttc ggcaatttcc ttgttagaaa aaccttcggc
     2161 aagcaactcc aggatttccc gttcgcgccg ggtgagatct tcgacttcaa tcgctctcgt
     2221 gagcggttcc tgaaacgaag cgatgacttt gcgcgcgatt tcccgagaca tgggcgcgcc
     2281 gccctggcgg acttcacgga ttgccgagac aatttcgtca ggcgagtaac gtttcaacag
     2341 gtagccgcag gcccccgcgc gcaacgcgtt aaaaatacga tccgtgtctt cataaaccgt
     2401 cacaacgatg atgcgaagag aaggcagcat ttgcttgagg cgggcggtgc attcgatacc
     2461 ggaaagattg ggcaattgga tgtccatcag cacgatgtca ggcgcaaatt tagggatcga
     2521 taccaaagcc tcttccgctg tggagcaggc gcatacgcaa cgattctcct gatccaaatg
     2581 gacaaattcc gcgaggcttt cacgaatgac cttgctgtct tccactatgg cgattttcgt
     2641 catgagctta aaggcaggat aaaccgcaaa gttgtgccgc tgccgatttc actgatcaat
     2701 tcgaattttc caccgagttt ttcgacgcgc cttcgcatgt taataactcc gttcatctcg
     2761 tcagtatgca tttctgctga aaagccgctc ccgttgtcca caatggtaag acgcagatgt
     2821 tcttcttcgg cgtgtatgct gattcgaact tcggaagcgt gcgaatgccg gacaatattt
     2881 gtcaaggcct ctttgaacgc cagaaacaat tgataccgat gccgggagtc cacaacgcgg
     2941 tcaggagccc ccaatggttt ttcgagccgc cacgatattt ttgccaaacc gagaaagcga
     3001 tccgcataaa gagagaaata actgaccagc gatcccagca cgttatgacg gggattcatt
     3061 gcccagacga tttcgtccag tgccgtgacc atttcctgcg acttttcatg cacctgctcc
     3121 aggtagcgat ttcttttttc atcgggagtg caagtggatt tcatcagcgc caccaacatg
     3181 cccatctctg taattccaga acccaggtca tcgtggaggt cttgcgctat gcgggcgcgc
     3241 tcctcttcca aggcgcgctg gcgttcggcg cgctcgcggc ttttaatttc tgccgtcaat
     3301 tgcaatgaac gttcttcgac ctggcggcgc agcgatgtga tccaaacaaa ggctgcggca
     3361 aggacactca acattgccgt catgctcatc acggcatggc gaattgtcca ccaagaggga
     3421 cgcgtgagca cttggatgtc tgccggggaa ttgagcaaca gttcgaatga atcaatgtca
     3481 cgactcactg tccgatccac ccgttgtcca atatagacac ctgtcagttc cagttcacag
     3541 ccgggttcga gtttggtgac aagcccgccg cctctctgca accgggcaaa ataactccgg
     3601 tttcccaatt gtaattccag ggtctgctcg gccccctcca atgccgatcc gatcaggcgg
     3661 gatttgatcc gcaccaaagt ggcatccaga tcggggttca acatattttg ttcaaccagc
     3721 cgcttcggaa tgggcaaatc agccttgccc gtgcggcgag caactccttc gcgaaggacc
     3781 ggggacgggc cgtttcggtc tggaaaaccc acaacttcca caaacgttcc caccggcaat
     3841 tggagcgctg ctttaggacg gaaacgaaag ccgtgggtcc cgtccatcag aaaaaattct
     3901 ccatgatatt cgcgcatgac ctgcccggaa attttgacgc gtcgaagcgt atcggctcgg
     3961 gcgtcaaaga aaaacaaatc gttggccttt ttgagcggag ctgaaaacgg atctgctggc
     4021 gctggttcat ccactttaac cgacgcattg tatatgcgaa ggcgttgccg catcatttga
     4081 tgttcatctc gatccgagct gccaactccg gaaatatgaa tgagagcgtc ttgcaagcct
     4141 ttgagttcgt cgttctccaa atcgacacac aaaatggtaa tcttgccgcc acgagtcaat
     4201 agtacgaatt cgttggaccg gatttgggag actactcctt ggatctccat gtatttggtg
     4261 cttaatgagc cgttgatcaa ttcatcccaa gtaggtcgga tcggcgcggg gagaattccc
     4321 ggcccgagat aaaccgcgtg tttaaccttt atatcaggtg caaaatccgc gctggaaatg
     4381 ccttcgattt gccagcactc tccaattctc ggcagccttc caccactaaa ggtgttcaag
     4441 tcaccgacgt aaatcgacca cgtcgagtcc tgaatgaaaa aattaacgtc cacatgggcc
     4501 gtcactactc cttgaagccg cactggatag ccgcgcaacg cttccttccg tccaagattc
     4561 atgacttgga gcgccgtagt cagcaacggc aatccgttcg aggcttccaa tgaactctgg
     4621 atgggttgcg ggtcaaggat gacgatgtca tccttgttcg gcaccaggag agaaggaact
     4681 ttttgtccat cgactgaaag ggcactctga taaataccgg tgatcttgac tcggtgatga
     4741 agaagaaagt ccaacgattc aggaccgcac tccatgactt tcaagtaagc ccggccggtg
     4801 ccagaattta tttccaaatt tggtgaaatt gagggaccgc gaataaccga aaccattccc
     4861 tccatctcag accagcgccc ctcctgtgtt tcttgaacga gttgacccgg tataacaggt
     4921 tccgggaacg gcatcgctgt gttacccaaa accctcactc cgagcggtga atctcctaca
     4981 tagagtttgc tgccatcatc tgaagttgtc caaaaatggt aaactccatc ccgcaacgcc
     5041 tcgaagtacc ctgtgaaaac aacccccacg ttcgtgtcgc gagtgcggat tccaatatcg
     5101 aaattggaga tggttccgct tttcgaaacc ggcagccgtg aaaagtctgg caaccacgac
     5161 cacgcaccct caaaaacttg atagaccaag ccgtttgtca attggccggt cttgttgtct
     5221 cgtagaaata aagcggcatc gggaatggat tcacgcttca ttccagggca ttggcagtcg
     5281 attttcaacg cgaacttccc gacgttgttg aaccactcga cccgaattgg atgaaaaccg
     5341 gcagaaagat aaattttgcc tgattgttcg tataacgcgt gggtcccatc gttattcacc
     5401 accgctatcg atgaaccggc ttcgccttga cctgcgcggc aagtgccctc gatgaggact
     5461 cgttgtccag gtttcgccgg agtctccaag gaatgtaaat ccacctttaa aatgaatccg
     5521 ccggaatcgt cctgaatggc caattgattt tgggtggagc taacccataa aatcactcct
     5581 tcgagccgta tggagcaact tacgatccct tgttgtgaag ccagacgacg caactgaagc
     5641 gtattcgtca cagtgtctgg aggcgtatct ctggggctgt ttgctgaccc gattgccgca
     5701 ttggccccgc caaaaataat tatcagccat aaaattgtca gcatggagag aagcccgtgc
     5761 ttgccgtgca cggtagtaca ttcgcttgct cgtggctttt cgttcggacg catgggaaca
     5821 accatcagtc tgaggtggat ccagcgaaaa tcaatccgaa ataaacacta acagtaactc
     5881 ggggtgtgat taaagtgggt gaggggaaaa gaatttgtgc gaataaaatg tgcaaattaa
     5941 gccattaact tacaacacat ctcataaata aaggagcgcg gctgtgcgcg aagcgccagc
     6001 cgcagcagct atgaacgccc cgagcgtttc aggctgctgc ggctggtcct ggcgggcaca
     6061 gccgcgctcc gttccctatt tatgagatga cttcaaatcg ctcacaccgc gtcctcagag
     6121 gcaatcgtca gtggcaatct ccatggaact gaatctcacc cacttttaat cacactcccc
     6181 aatgctgaaa acgccaatcg ggtccgccag ccaaattttt tctggctatt gggccgctct
     6241 gcttctgtaa tgggcgcgat tcatgaacag ctacttcaac gcggcggatt ggtgggtcat
     6301 tttcctctac ctcggaggca tcatcttgct cggcgtctgg ttcggcaagg accagaagaa
     6361 cacccgcgat tattttctcg gcagcagaaa cattccctgg tggggcatcg gactttccat
     6421 tgtcgccgcc gaaaccagcg cgctcaccat catcggtgtc ccctcgatgg cttacggcac
     6481 caacatggca tttctacaga tgattatcgg ttacgtcatc gcgcgtgtca ttcttgccat
     6541 cgtcatggtg ccgcattatt tcaaaggcga aatctattcg ccgtaccaac tcttttccaa
     6601 tgcgttcggc ccttcggtga ggcagatggc aggcggtttt tttctgctca gcgaaacctt
     6661 ggccgcgggc gtgcgcgttt atgtcgcctg catccccatc aagctgatgc tcggtgacaa
     6721 gttcctcggc ttcctgacga acgatcccat ccttggcgca attttgttgt tcgtgctttt
     6781 gtcactgctc tacacctaca ttggcggagt caaggccgtc atctggacgg atgcggtgca
     6841 gttcggtttg tttttggcag gtgggctttt cacgcttttt tatattccaa gttttttcaa
     6901 aggcggtgcg aaggaagttt ttcatctcgc gtcggcaggc ggcaaattgc attggctgaa
     6961 tggcgcgttc accctcggcg cgcccatcaa catttggatg ggcatcatcg gcggaacggt
     7021 catggtcatg tcatcgcacg gcgcggagca attgatcgtg caacgggtgc tcgcctgcaa
     7081 aaacgtggcc gatgggcgca aggcgctcgc gttgagcgcg gtcatcatct ttccgctctt
     7141 cctgattttt cttttgtgcg gcgcgatgct ctgggttttt taccaggcgc atccgttcca
     7201 aatcccgctg cccgaagccc gcgcaggcat caagtccaac gatttcattt ttccgatttt
     7261 catgatgacc gaggtgccgc atgtgctgaa agggtttttg atcgtggcaa tcctttctgc
     7321 cgcgatgtca tcggtcagtt cggcgctgac gtcgctctcg tcggtttcga ccatggattt
     7381 tgtgaaggcg ctttcgcgaa agcagcggag cgaggaattt tatttgaaat tcagcaaaat
     7441 ttcgaccatc gcctgggcca taatgctgat attagttgcg tacattagcc ggagggttga
     7501 attcgtgctg aacgcgtcgt tttcattgcg tggattgacc agcggcgcgt tgttgggcgg
     7561 attgttgctg gcagtttttt ggaaacgagg ccgtgccacc ccgattctaa ttgggatggc
     7621 ggtgtcgttg ctggtgatga ttgccattca agtcttgccg aactatttcc ccagcattcc
     7681 cgcgatattc tggccgtggt atgcgttgat cggcacaact gtcatggtgc tcgtttcgtg
     7741 gtcggtgcgg gaaatgatgc cggcacacga ggaagagcaa aagaccggca cgcccgagcg
     7801 gaagattgga gcagtggatt agcggagcag aaaaacaccc ctttttgcgt gtagggaaca
     7861 aagattttgt cctacacgcc aaaaagtcgt gttttttgaa aagggagaaa cccgcatgga
     7921 cattggtcga aacggctgaa aactcacgtt ttcagctaca tcaaaaaaaa tgctgtcata
     7981 cctaccgtag gtttcatttt ttcggggagg ccatggtaat ctcgaaccat gatagttgaa
     8041 gatggtaaat tggagtgttg gagtgaggga gaatggggcc gatggagtat tggagcaa
//
LOCUS       DASZMM010000202         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1188901, whole genome shotgun sequence.
ACCESSION   DASZMM010000202 DASZMM010000000
VERSION     DASZMM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188901"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="scpB"
                     /locus_tag="VG754_04845"
     CDS             <1..679
                     /gene="scpB"
                     /locus_tag="VG754_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWF18568.1"
                     /translation="MELKFILESLLFSAQKPMSARELRDVLTEAAASAEGDETVRALK
                     KVKEEALVTALEELSREHETAARSYRLVCVAGAWQFVSQPEYAPWLKALVGHKIRPGR
                     LSQPALETLAIIAYRQPLTRAEIEQVRGVAVDGVMQTLVERGLVEQVGRAEVIGRPMT
                     YGTTSSFLEYFGLRSLEDLPAADELRRIPVVKPESLVTADPGLATVPPDQLQLPQAET
                     PPTPKEP"
     gene            752..1813
                     /gene="pheA"
                     /locus_tag="VG754_04850"
     CDS             752..1813
                     /gene="pheA"
                     /locus_tag="VG754_04850"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWF18569.1"
                     /translation="MNIPEHRKAIDKLDAQIVHLLNERTRHVLEIGDIKLKAGEEIYA
                     PHRERAVLQRVCKLNTGPITEESLRAIYREIMSSALSLEKTMTIAYLGPEATFTHQAA
                     IRRFGASLHYASQKTIADVFLEVTKRRADYGVVPVENSTEGVVTHTLDMFVDSNLKIV
                     AQIILPIQHCLVSNCKRDELKKLYAHPQSFAQCRIWVQNHLPHIEIIETSSNARSAEL
                     AAKEAHSAAIAGVLAAERYGLRILESDIQDNSANATRFLVLGRQCSPPTGKDRTSIML
                     SITHEVGALYRALASFRKFKVNLTKIESRPSKRKAWEYYFFVDCEGHKEDRKVAKAIA
                     ELERQCNFVKVLGSFPNGE"
BASE COUNT          445 a          567 c          545 g          387 t
ORIGIN      
        1 catggaactg aaattcattt tggaatcatt gctgttctcg gcgcaaaaac cgatgagcgc
       61 gcgcgaattg cgcgacgtgc tcaccgaagc cgctgcaagc gccgaaggcg acgaaaccgt
      121 ccgcgcattg aaaaaagtca aggaagaggc gctggtgacc gcgctggaag aattgtcgcg
      181 cgaacatgaa accgcggcgc ggagttaccg gctcgtttgc gtggcgggcg catggcaatt
      241 cgtcagccag cctgaatacg cgccatggct caaggcgctt gtcggccaca agattcgtcc
      301 cggacggctt tcgcaaccgg cgttggaaac gctggccatc atcgcctatc gccagcctct
      361 cacccgcgcg gaaatcgagc aggtgcgtgg cgtggctgtg gatggcgtga tgcaaacgtt
      421 ggtcgaacgc ggtttggtcg agcaagtcgg ccgcgcggaa gtcattggtc gtccgatgac
      481 ctacggcacc acatccagtt tcctggaata tttcggcctg cgcagtttgg aagatttgcc
      541 cgccgccgat gaactgcgcc gcattcccgt cgtcaaaccc gaatcgctcg tcaccgccga
      601 ccccggtttg gcaactgttc cgccggatca attgcagttg ccgcaggcgg aaacaccgcc
      661 aactccaaaa gaaccgtagg ccatcgcaag cccgacaggg actcgatttt tccctgccct
      721 tcgactggat gggactatat agtatgcctc gatgaacatt cctgagcatc gcaaggcgat
      781 tgacaagctg gacgcgcaaa tcgtccatct gctgaacgaa cgcactcggc acgtactgga
      841 gatcggcgac atcaagctca aggccggcga ggagatttac gcgccgcacc gcgaacgcgc
      901 ggtgttgcaa cgcgtttgca agctgaacac cgggccgatc accgaggaat cgttgcgggc
      961 aatctatcgc gaaatcatgt cgagcgcgct ttcgctcgaa aaaaccatga ccatcgcgta
     1021 tctcgggccg gaagcgacgt tcacgcacca ggcggccatc cggcggtttg gcgccagcct
     1081 gcattacgcc tcccaaaaaa ccatcgcgga cgttttcctc gaagtgacga agcgccgtgc
     1141 agattacggc gtcgtgccgg tggaaaattc caccgaaggc gtggtcacgc acacgctcga
     1201 tatgttcgtg gacagcaact tgaagattgt cgcgcagatc attttgccca tccagcattg
     1261 cctcgtgagc aattgcaaac gcgacgaact caaaaaacta tacgcgcatc cgcaatcctt
     1321 cgcgcaatgc cgcatctggg tgcagaacca tctgccgcac atcgaaatca ttgagacttc
     1381 ctcgaacgcg cgttccgccg aactcgccgc taaggaagcg cattccgcgg ccatcgctgg
     1441 cgtgcttgcg gcagagcggt atggtttgcg cattttggaa agcgacatcc aggacaattc
     1501 ggcgaacgcc acacggttcc ttgttttggg acgccaatgc agtccgccga cgggcaagga
     1561 ccgcacgagt atcatgttga gcatcacgca tgaagtcggg gcgttgtatc gggcgctcgc
     1621 gtcttttcgc aagttcaagg tcaatttgac caagattgaa tcgcgcccca gcaaacgcaa
     1681 agcttgggaa tattatttct tcgtcgattg cgagggccat aaggaagacc gcaaggtcgc
     1741 caaagccatc gccgaactcg agcgccaatg caatttcgta aaggtcctcg gttcttttcc
     1801 aaacggcgag taaattggtt ccgagtcaat tttgaacttt aaacaacact cttcaaactg
     1861 ctaatctgcc tccatggcta aaaaggctgc gccggattcg gctgggcacc gcgggcggaa
     1921 cgacatcatc ggcattgtgc tgat
//
LOCUS       DASZMM010000203         6730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3098554, whole genome shotgun sequence.
ACCESSION   DASZMM010000203 DASZMM010000000
VERSION     DASZMM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3098554"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1263)
                     /locus_tag="VG754_04855"
     CDS             complement(292..1263)
                     /locus_tag="VG754_04855"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HWF18570.1"
                     /translation="MKLRTLFWMDHRSMAVGIAVVLALPTCLMAADEQRFDSPEAAVA
                     ALTNAATAKDTNAIRDIFGPEGHALVSPDAVQANENFNHFVTRVTEKTRLVPMGEGRD
                     ELEIGSEGWPFPIPLVKEDAKWYFDVAAGREEILNRRIGSDELGAMNVCHAYVDAQRE
                     YASRDHSGNDVLQYAQNLRSTPGKHDGLYWPVTNDNEQLSPLGPLITEAREEGYHRHT
                     GMMTDQNTQTPYHGYYYKILTRQGSHAPGGKYDYIINGNMIAGFALVAWPAEWNNTGV
                     MTFIVNQQGKIYQKNLGPKTDAIAKHMTAYDPDPSWTLVGTLQNASR"
     gene            complement(1289..2398)
                     /locus_tag="VG754_04860"
     CDS             complement(1289..2398)
                     /locus_tag="VG754_04860"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HWF18571.1"
                     /translation="MKGFSIVVSSLVIAGAIQVGAQNYYPTAAPPPAPSGQVFAGAQL
                     DQLLGPIALYPDPLIAQILSAATQPSQIAVAANYVNSGGDPNQIDAQPWDTSVKAVAH
                     VPQVLQLLDGNLQWTAELGQAFANQPTDVMNSVQRLRAEAMQQGNLQNNQYDTVENDN
                     GEIEIVPTNPDTIYVPQYDPGLVYYQPGVSFGWGLGFGIGPWLDFDFDWGHHHIVEWD
                     RDHPRPGDWWHERPEQRRAFIGRAPEWRPEEHRGGGGASRQFFSSVNRGGDRGYEAPR
                     ETRPAFSAPAPREGRPSFTPRESVPAPRETRPTFAAPAPRPAMPARPSAPSMFGGGNP
                     SETRAASSRGMESRGVSHPAVGGGGGGGRGGNQRK"
     gene            2547..3527
                     /locus_tag="VG754_04865"
     CDS             2547..3527
                     /locus_tag="VG754_04865"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWF18572.1"
                     /translation="MKICWILLFGMALAVSALRARAESSAVADSILAVVGDTPITQQQ
                     VEQLIDQDQQALAQRYGNQPDIFYKKVASLQESGSELLIRREVILQDFKQNIKVPDSI
                     IEEYVQDEIKKRFPGVNGNIELTKQLELEGLTPEQFKKRIRDQFIVNAMQDKFVTDPI
                     ISPKKIEDYYMAHRNDFKLEDQVRMRMIVLGKVSADDTNVVEQTRRRASELVSQLKGG
                     ATFADLARTYSEGSAAKDGGDTGWEDASVVNKLLVAELDKLKPGEYSGVIEAPDGFYL
                     VMLEDRHPAHYKPLNDVREQIERTLDSAERERLAIKWINRLKEKTFILKF"
     gene            3693..4688
                     /gene="pdxA"
                     /locus_tag="VG754_04870"
     CDS             3693..4688
                     /gene="pdxA"
                     /locus_tag="VG754_04870"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWF18573.1"
                     /translation="MSSRIGISLGDVTGIGPEVILKALAAEAQADDSHYLLIGDFQLL
                     DRLNRQFGLQLPLQMAEKNSGRFLVQNPLTGALPANLQPGSPVAAQAAVAWLKDGAQR
                     CLRRELDALITAPVNKESIIDSGERSFIGQTEFLSQLAGTTHTGMMLMGQDDRGRWLR
                     VVLATTHVPLRLVAEQLTQAKVELAIELAAKACADLDLPKARVAVCGLNPHAGEGGKM
                     GTEEITTIAPAVEAARRKKLNVSGPFAADALFYSVFKGDYEIVVAMYHDQGLAPLKMI
                     GFESGINWTLGLPFIRTSPDHGTAYNIAGKGIANPSSMRAAIQLARQLAAKRARS"
     gene            complement(4739..5704)
                     /locus_tag="VG754_04875"
     CDS             complement(4739..5704)
                     /locus_tag="VG754_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HWF18574.1"
                     /translation="MPLTHTKELFAKALKGKYALGAFNVNNMELLQAIVEACEEEKAP
                     FMLQISKGARQYASPVYLRKLIEAAVSLSNVPLAVHLDHGDTFELCKECIDEGFTSVM
                     IDGSHEPFEKNVELCRRVVEYAHKHDCVVEGELGMLVGAQHDDGEEGGGYSKGGVYTH
                     PQEAVEFVRQTGVDSLAVAIGNSHGAYKFKGEQHLDLDRLKAIKHALMEAGMGDYPLV
                     LHGASSVPKDLVEEINKYGGKMGEETAGVPESDIEVARRIGCTKVNIDTDLRLAMTAG
                     IRKFFAENPKEFDPRKYLAPARARVKELVRHKVCHVLCSAGHAFD"
     gene            complement(5695..6129)
                     /locus_tag="VG754_04880"
     CDS             complement(5695..6129)
                     /locus_tag="VG754_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18575.1"
                     /translation="MKPIVPSLTRPCLCHPPSSFGVCRLASLQEKAAGDCRTPRRYRD
                     FQASSEFREVFGFAILYPLFRRLCRLAVKPCAFSPILRHSNTPLFRIHACTRLFTLNF
                     EKYFAATRDSHAEISLFFAARLRIIRFQFPMNFTGMIIELCH"
BASE COUNT         1553 a         1835 c         1893 g         1449 t
ORIGIN      
        1 tttgcccatt tggaaacacc gcagtccgcg gacatcgcaa cgcgatgtcc ctaccagccg
       61 atgggaggac gtaagacgcg acaaggatga tccattttgg acaaaagcga tgatttccgg
      121 cgaatgaagg gccgtttggc gacagacacc taacggtgtc ctctgttgcg ctccttgtct
      181 cacgcggttg cagtatttca cataaaagcc ggatgtccag ctttttcgtt taagcgacga
      241 tgtgagggcg caaagaaacg gcgaaatttc aattcaacct tccaaccgac tttaccggga
      301 agcgttctga agagtcccca ccaacgtcca tgaaggatcc ggatcgtaag cggtcatgtg
      361 tttggcgatg gcatcggttt ttggaccgag gttcttttga taaattttgc cttgttgatt
      421 gacgatgaaa gtcatgacgc cggtgttgtt ccactcggct ggccatgcca ccagcgcaaa
      481 cccggcgatc atgttgccat tgatgatgta atcgtatttg ccgccgggcg cgtggctgcc
      541 ttgacgggtg agaattttat aatagtaacc gtgataaggg gtctgggtgt tttggtcggt
      601 catcatgccg gtatggcgat gataaccttc ctcgcgcgcc tcggtgatga ggggacccaa
      661 cgggcttaat tgttcgttgt catttgtgac cggccaataa agtccgtcat gctttccggg
      721 ggtgctgcgc aaattttggg cgtattggag cacgtcgttg ccactgtggt cgcgcgaggc
      781 gtattcgcgt tgggcatcca cataggcgtg acagacattc atggccccaa gttcgtccga
      841 gccgatgcgg cggttgagaa tctcttcccg tccagcggca acatcgaaat accattttgc
      901 atcttccttc accaacggaa tgggaaacgg ccagccttca ctgccaattt ccaattcgtc
      961 ccggccttcg cccatcggca ccaagcgcgt tttttcggtc acgcgcgtca caaaatggtt
     1021 gaagttctcg ttcgcctgga cggcgtccgg tgaaaccagc gcatgtcctt ccgggccgaa
     1081 aatatcgcgg atggcattcg tgtcctttgc agtggcggcg ttggtgaggg ctgcgactgc
     1141 ggcttcaggc gaatcaaaac gctgttcgtc agcggccatc aagcaggtcg gcagcgccaa
     1201 caccacggct atgccaaccg ccatgctgcg gtggtccatc caaaataaag tgcggagttt
     1261 catgtgctga accttgtgga aaaccatatc atttgcgttg attgccacca cggcctccac
     1321 cacctccgcc gccgaccgcg gggtgactga caccacgact ttccatgccg cgactgctgg
     1381 ctgcgcgggt ctccgatggg tttccaccgc cgaacatgct gggagccgaa ggtcttgcgg
     1441 gcatggcggg acgaggagct ggagcagcga aggtcggcct tgtttcacgg ggtgcaggga
     1501 ctgattcgcg cggtgtgaaa cttggccttc cctcacgggg cgctggggcc gaaaaggccg
     1561 gacgtgtttc acgcggtgct tcgtatccac gatcgccacc acgattcacg gaggaaaaaa
     1621 attgacggga cgcgccaccg ccgccacgat gttcttctgg acgccattca ggtgcgcggc
     1681 caatgaaagc acgccgttgc tcaggccgtt catgccacca atctcccggc cgcggatggt
     1741 cccggtccca ttcaacgata tgatggtgac cccaatcgaa gtcaaagtcc aaccaggggc
     1801 caatgccaaa tccaagcccc caaccaaaac tcacacccgg ctgataatag acgaggcccg
     1861 gatcatattg cgggacgtaa atcgtatcgg gattggtggg aacgatctca atctcgccat
     1921 tgtcgttctc gactgtgtca tactgattgt tctgaagatt gccttgctgc atggcttcag
     1981 cacgaagacg ttggacggaa ttcatcacgt ctgtcggttg atttgcgaat gcttggccca
     2041 actcggcagt ccattgcaaa ttgccgtcca acaattgcaa tacttgcgga acatgagcga
     2101 ccgccttgac gctggtgtcc cacggctgcg cgtcaatttg gttcggatcg ccgccggaat
     2161 tcacataatt cgccgcgacg gcgatttgcg atggctgggt ggcagcggat agaatttgtg
     2221 cgatgagcgg atcgggataa agggcgattg gcccgagcaa ttgatcgagt tgggcgcccg
     2281 caaatacttg gccggaagga gccggtggcg gagcagcggt cggataataa ttttgcgcgc
     2341 caacttgaat ggccccggca atcaccagcg atgaaaccac aatgctgaat cccttcatca
     2401 gcgctaactt cgacgagttt tgttctggag tcaatactcc atgggagaat tatattgaaa
     2461 ggagaatgat agacttcagc aagcctaaga aaagcccaac agcgcttgac ccgctctggc
     2521 gcgttcccta tggtttgcct cgtcgcatga aaatctgttg gatcctgttg tttggaatgg
     2581 cactggctgt ttccgcgttg cgcgcgcgcg cggaatcgtc cgcagttgcc gacagcatcc
     2641 ttgcagtggt cggcgacaca cccatcacgc agcagcaggt cgaacaactg atcgatcaag
     2701 atcagcaggc gctcgcccag cgatatggca accagccgga tattttttac aagaaggtgg
     2761 cttcgttgca ggaatcgggg tcggaactat tgattcgccg cgaggtgatt cttcaggatt
     2821 tcaagcagaa catcaaggtg ccggacagca tcatcgagga atatgtccag gacgagatca
     2881 agaaacgctt ccccggcgtg aacggaaaca tcgaactcac caaacaactc gaactggaag
     2941 gcctgactcc cgaacaattc aagaagcgca tccgcgacca attcattgtg aatgccatgc
     3001 aggacaagtt cgtcaccgac ccgatcattt cgccgaagaa aatcgaggac tattacatgg
     3061 cgcaccgcaa tgatttcaaa ctcgaagacc aggtcaggat gcggatgatt gttttgggaa
     3121 aagtctcagc cgacgatacg aatgtcgttg agcaaacacg ccgtcgcgca tcggagctgg
     3181 tatcgcaact caaaggcggt gcgacgttcg cagatttggc ccgcacctat tcagaaggtt
     3241 cggccgccaa ggacggcggc gataccggtt gggaagatgc gtccgtggtg aacaagttgc
     3301 tggtggcgga actcgataaa cttaaacccg gcgaatacag cggtgtcatt gaagcgcctg
     3361 atggttttta ccttgtgatg ctcgaagacc ggcatcccgc gcattacaag cccttgaacg
     3421 atgtccgcga gcagattgaa cgaacgctcg acagcgccga acgggaacgg cttgcaataa
     3481 aatggatcaa tcggctcaag gaaaagacgt tcatcctgaa gttttgagaa cggaacgcgg
     3541 ctgtgtccgc aaaaccagcc gcaacaggtt ggaaccatca ggttttcgat aagttgctgc
     3601 ggctgccgct tcgcgcacag ccgcgctcca caaacaccgt tgcggcatgt gactttgtaa
     3661 cgatttacca tttaactctg taaccattaa caatgtcttc ccgcatcggc atctcattgg
     3721 gcgatgtcac cggcatcggg ccggaagtca tcctgaaggc tctggccgcg gaagcgcagg
     3781 cggacgattc ccattacctg ctcatcgggg atttccaact gctcgacaga ttgaatcgcc
     3841 agttcggatt gcaactccca ttgcagatgg cggaaaaaaa ctccggccgt tttctcgttc
     3901 aaaatccact caccggagca ttgcccgcaa atcttcaacc cggttcgcca gtggcggcac
     3961 aagcagccgt tgcatggtta aaggatggcg cgcaacgttg tttgcgccgc gagcttgacg
     4021 ccttgatcac cgcgccggtc aacaaggaat ccatcattga ttccggcgag cgttcgttca
     4081 tcggccagac agaattcctt tcgcaactcg ccggcaccac gcacaccggg atgatgctca
     4141 tggggcagga tgatcgcgga cgctggttgc gcgtggtgct tgccacaacg cacgtgccgt
     4201 tgcgattggt cgccgagcaa ttgacacagg cgaaggtgga actcgccatc gaacttgccg
     4261 cgaaagcttg cgccgacctc gatttgccca aagcgcgtgt cgccgtgtgc ggattgaatc
     4321 ctcacgcggg cgaaggcggc aagatgggca cggaagaaat cacaaccatt gcgcccgcag
     4381 ttgaagcggc gcgccgaaaa aaactgaatg tctccggccc ttttgccgcc gacgccttgt
     4441 tttattctgt gttcaaaggc gattacgaga ttgtcgttgc gatgtatcac gaccagggac
     4501 tcgcgccgtt gaagatgatc ggcttcgaaa gcggcatcaa ctggacgctc ggcctgccgt
     4561 tcatccgcac gtcgccggac cacgggaccg cttacaacat cgcgggcaaa ggcatcgcaa
     4621 atccttcgag catgagagcg gcaatccaat tggcgcggca actcgccgca aagcgcgctc
     4681 gttcgtagct gaaaacgtga gttttcagcc ggcttaaaat ttcaaagagc aacaaaactc
     4741 agtcaaacgc atgtcccgcg ctgcaaagca cgtggcaaac tttgtgccgc accaattctt
     4801 tcacgcgcgc gcgggctggc gccaaatatt tgcgcggatc gaactctttc gggttttcag
     4861 caaaaaattt gcggatgccc gcggtcatcg cgaggcgaag gtcggtgtcg atgttcacct
     4921 tcgtgcagcc gatccggcgg gcgacctcaa tgtccgactc cggcacgccg gcggtttcct
     4981 cgcccatttt gccgccgtat ttgttgattt cctccaccaa atctttcggc acgctggatg
     5041 cgccgtgcaa caccagcgga taatcgccca tgcccgcttc catcaacgcg tgcttgatcg
     5101 ccttgagccg gtcgaggtca agatgctgct cgcccttgaa cttgtaagcg ccgtgcgaat
     5161 tgccgatcgc caccgcgagt gaatccacgc cggtttgccg gacaaattcc accgcttcct
     5221 gcggatgcgt ataaacgccg cccttggaat aaccgccgcc ttcctcgccg tcatcgtgct
     5281 gcgcgccgac cagcatcccg agttcgccct cgacgacgca gtcatgcttg tgcgcgtatt
     5341 cgacgacgcg ccggcaaagt tccacgtttt tttcgaacgg ctcatggctg ccgtcaatca
     5401 tcacgctggt gaaaccttca tcaatgcatt ccttgcacaa ctcaaacgta tcgccgtggt
     5461 ccagatgcac ggccagcgga acatttgaaa ggctgacggc ggcctcgatc aatttgcgga
     5521 gataaacggg actggcatat tgccgcgcgc ccttggaaat ctggagcatg aacggcgctt
     5581 tctcctcctc gcaagcctcg acgatggcct ggagcaattc catgttgttg acattgaaag
     5641 cgccgagcgc gtatttgccc ttgagtgctt tcgcgaacaa ttctttggta tgggtcagtg
     5701 gcataattcg ataatcattc cagtaaagtt cattgggaac tggaaacgga taatacgaag
     5761 ccgggcggcg aagaaaagtg atatttctgc gtgagagtcc cgggtggccg cgaaatattt
     5821 ttcaaaatta agcgtaaata agcgagtaca agcgtggatg cggaacagtg gagtattgga
     5881 gtggcggagt attggtgaaa aagcgcatgg cttgaccgcc aagcgacaca gtcgccggaa
     5941 cagaggatag aggatagcaa agccaaagac ctcgcggaac tccgacgatg cttggaagtc
     6001 gcgatagcgt cttggagtgc ggcagtcccc tgccgctttt tcctgaagcg acgccaagcg
     6061 acacacccca aacgaggatg gaggatggca aagacatggc cgcgtgagcg atggaacgat
     6121 gggtttcatg caaaataatt tattttttta ccccccttta tctgaaaata actgaaaata
     6181 actgaaaata agtgaaaata actgaaaata gctgaaacga tctgaatgaa taagtgaaac
     6241 aatgcctcga aagcagcgga aaagccgaaa cgctgtgtgc tccggaggag tgcaggagat
     6301 tagccagaca caaagtgtct ggtcgcgcga aatgactgtt ttcgtcccgc gccgtcttga
     6361 tgacgaactg gctgtctcaa gcaacccgga gggttgcaag aaattagccg ggggttgagc
     6421 gaggcgacac ccccggaaaa tgtcgcgtaa accgatgcac cctgaagggg tgccagaacc
     6481 aacgaaccaa ttgcgcttct gccacccttt cagggtggaa aattggacaa cagacaaccg
     6541 ggggtggcgg cctgcggcct tacccccggc taatttcttg caagcctcca gcttgcggag
     6601 cagcaactgt ccctgcggga cgagcgcggt ggcgcttccg cgtggccaaa gcgatgttga
     6661 cgaccttggc gagcgggcgc atctcagttt ttagaggggt cgatgcgccg atgatgggga
     6721 aagagaaggt
//
LOCUS       DASZMM010000204         3711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1730040, whole genome shotgun sequence.
ACCESSION   DASZMM010000204 DASZMM010000000
VERSION     DASZMM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730040"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..303)
                     /locus_tag="VG754_04885"
     CDS             complement(49..303)
                     /locus_tag="VG754_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF18576.1"
                     /translation="MYRIFLEHAAEKDLRRLSSDIHNRVVAAIRALGNTPRPHGCRKL
                     TGSENNWRIRVGDYRVIYEIADVIRVVRVHRIRHRREVYR"
     gene            complement(284..502)
                     /locus_tag="VG754_04890"
     CDS             complement(284..502)
                     /locus_tag="VG754_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWF18577.1"
                     /translation="MKSLSHPEIVTRKGKPVSVILPIKVYEELLERAEDAEDVRWLNK
                     ARRRPLRFRPLEDYLTERKEKKRVQNLP"
     gene            complement(592..3129)
                     /locus_tag="VG754_04895"
     CDS             complement(592..3129)
                     /locus_tag="VG754_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18578.1"
                     /translation="MTKTEKSSPAQAQSASPVLPPGYHVMVLLVDDQAMVCEAIRRSL
                     ANQSDIDFHYCADPEEAIVVANQIKPTVILQDLVMPGVEGLALVGRFRANPATRDTPI
                     IVLSTNETPQVKGQAFAAGANDYLVKLPDGVELIARLRYHSKSYLNQLQRDEAYRALR
                     ESQKQLVETNATLVALNHKLEDATLAKSEFLANMSHEIRTPMNGVLGMTALLLDTELT
                     DEQREYVEATRSSADAMLTIINDILDFSKIESGKLELESHPFELHTCIEEALDLLAPK
                     AAEKSLDLAYLVEDSIPKILVSDVTRLRQILVNLIGNAVKFTHEGEVVIEVKPAARGV
                     RTPEPGKERDTDFIRHPEEWLLHFSVRDTGPGIPAEKQHRLFKSFQQVDASTTRHYGG
                     TGLGLAICSRLSELMGGKIWVESDAGKGATFHFIIKVKSAASVVAPAWQSFQPQLAGK
                     RLLIVEDNATNAQIIAHRSKLWGITAEIVTHARDALTLLAQNTPFDAVIVDLQLPGTD
                     GLALAEEIRTLPRGRSLVLLLLSSVRVRSDDPRPQLAGISVFVHKPIRPAQLLEALYR
                     AMSIQLQREKRAPVSPSIDATFALRFPLRLLLADDNPINQKVGLSVLSKLGYRADVVQ
                     NGLEVIRAVEQKPYDIILLDVQMPEMDGLEATRTICKRWPEAKRPRIIAMTGNALLGD
                     REKCLEAGMDDYISKPVRISELQAALERWASRISKRSDTAFFTQQGITTNLIDQSLLE
                     ELRGVDADGANMLNELIDLFLQHAPQRIAQINQFLADPQKMAFHAHALKSMSVNLGAV
                     RIGELCQKLEEMAHSGNEEGVARLAQELDRTFLRTQAELAPFRTQEK"
     gene            complement(3155..>3711)
                     /locus_tag="VG754_04900"
     CDS             complement(3155..>3711)
                     /locus_tag="VG754_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWF18579.1"
                     /translation="VRSVRGNIRIATQPGKGMRFQLQLPLTLSVVRALLVEIAGEPYA
                     IPLSQTSRALKLPMNQIATLEGRPHFTLANQQIGLLTARQILERGEISADDDEISVVV
                     LGDRANHRHGLVVDRFLGERELVVQALDPRLGKVRNISAGAFMEDGSPLLILDVEDLL
                     RSIDELVAGGHIAAFAARQSQPRS"
BASE COUNT          791 a         1035 c         1035 g          850 t
ORIGIN      
        1 ccgagcaaat ctccggcgtg cagacgttcg ggcagggtgg gcataatttc aacgatatac
       61 ttcgcggcgg tgacgtatcc ggtgcactcg caccacgcgg atcacatccg caatttcata
      121 aattacccgg tagtcgccta cacgaatacg ccaattattt tcgcttccgg tgagttttcg
      181 acaaccatgc gggcgagggg tgtttcccaa agctcgaatg gcggcaacga ctcgattgtg
      241 aatgtcggat gacagccgtc gcaaatcctt ttccgctgcg tgttcaagga agattctgta
      301 cacgcttttt ttctttgcgc tcggtcaaat aatcttccaa tgggcggaaa cgcagagggc
      361 gtcggcgggc tttatttaac cagcgcacat cttctgcatc ttcggcgcgc tccaagagtt
      421 cttcataaac cttgatgggc aaaatgaccg acacaggttt gcccttgcgc gtcacaatct
      481 caggatgtga aaggcttttc atcaggttag gtgagaattc gtgtttctct catactgtaa
      541 tttcactgta tcaggatgcg aaggaaaggc aagctgccct ccaagacccg ttcacttctc
      601 ttgcgttcgg aagggagcca gttccgcctg tgttcgcaga aaggtgcggt cgagttcctg
      661 ggcgaggcgg gcgacacctt cctcattgcc ggagtgggcc atttcctcaa gtttctggca
      721 aagttccccg atgcgaaccg cgccaagatt cacgctcatg cttttgagcg cgtgggcgtg
      781 gaacgccatc ttttgcgggt cggcaagaaa ttggttgatc tgtgcaatgc gttgcggcgc
      841 gtgttggaga aagagatcga tcaattcgtt gagcatgttg gccccatcgg cgtccacgcc
      901 gcgcaattcc tcgagcaacg attgatcaat gagattcgtc gtgatgccct gctgggtgaa
      961 aaaggcggtg tcggaacgtt tgctgatgcg tgaggcccaa cgttccagcg cggcttgcag
     1021 ttcactgatg cgaacgggct tggaaatgta atcgtccatg ccggcttcaa ggcatttttc
     1081 cctgtcgccg agcagcgcat ttccggtcat ggcgatgatg cgggggcgct ttgcttcggg
     1141 ccatcgcttg cagattgtgc gcgtcgcttc aagcccgtcc atttcgggca tctgcacgtc
     1201 cagcaggatt atgtcgtacg gtttttgttc gacggccctg atgacttcca atccattttg
     1261 gacaacatcg gcgcgataac caagcttcga caaaacactc aatcccacct tttggttaat
     1321 tggattatcg tcggccagca acaggcgcaa cggaaaacgc aatgcgaaag tggcgtcaat
     1381 cgacggcgac accggcgcgc gtttttcccg ctgcaattga atgctcatgg cgcgataaag
     1441 cgcttcgagc aattgcgccg ggcggatggg tttatgcaca aagaccgaga tgccggcgag
     1501 ttgcggacgc ggatcatcgc tgcggacccg cactgacgaa agcagcagca agaccaggga
     1561 acggccgcga ggcagggtgc ggatttcttc tgccaacgca aggccgtccg tgcccggcaa
     1621 ttgcaaatca acgatgaccg cgtcgaacgg cgtattttgc gccaggagcg tcaacgcgtc
     1681 acgcgcatga gtcacgattt cggcggtgat gccccagagt ttggaacgat gcgcaatgat
     1741 ctgcgcgttc gtggcgttgt cttcaacgat gagaaggcgt ttgcccgcaa gttgcggttg
     1801 aaaactttgc cacgccggcg ccacgacgga agccgcggat ttcaccttga tgatgaaatg
     1861 gaaagtcgcg ccttttccgg catcgctttc cacccaaatc tttccgccca tcaattcgga
     1921 caagcggctg caaatcgcca atcccaaccc cgtgcctccg taatggcggg tcgtcgaggc
     1981 gtccacctgt tgaaatgatt tgaacaggcg atgttgtttc tctgccggaa tgcccgggcc
     2041 tgtgtcgcgc accgaaaagt gcaggagcca ttcctcggga tgacgtatga aatcggtgtc
     2101 gcgctccttg ccgggttccg gagtgcgcac gccgcgcgcg gcgggcttga cctcgatcac
     2161 cacctcgcct tcatgggtga acttcaccgc atttccaatc agattcacca aaatctgccg
     2221 caaccgcgtg acatcgctca ccaaaatttt tgggatcgaa tcctccacca gatacgcaag
     2281 atccaaactt ttttcggcgg ccttgggcgc aagcaaatcg agcgcttcct caatgcacgt
     2341 gtgcaattcg aacggatggc tctccaactc cagcttgccg gactcgatct ttgaaaaatc
     2401 aaggatgtcg ttgatgatgg tgagcatcgc gtcggcgctg ctgcgggtcg cttcgacata
     2461 ctcgcgctgt tcgtccgtca attccgtgtc caaaagcagc gcggtcatgc caaggacgcc
     2521 attcatcggc gtgcggattt catggctcat gttggccaga aattccgact tggcgagcgt
     2581 tgcgtcctcc agtttatggt tcagcgcaac cagcgttgcg ttggtttcaa caagttgctt
     2641 ctggctttcg cgaagggcgc ggtaggcttc gtcgcgctgc aattgattca gataactttt
     2701 cgagtgataa cgcaaacgcg cgatcaactc gaccccgtcc gggagcttga cgagataatc
     2761 gttcgcaccg gcggcaaaag cctggccttt gacctggggc gtttcattcg tggaaaggac
     2821 gatgatgggc gtgtcgcgcg tggcgggatt tgcgcgaaaa cggcccacga gcgccaagcc
     2881 ctcaactccc ggcatcacca aatcctgcaa aatcaccgtg ggcttgattt ggttggccac
     2941 gacgattgcc tcctcgggat cagcgcaata atggaaatca atgtccgact ggttggccag
     3001 gctgcgccgg atggcttcgc agaccattgc ttgatcatcc accagcagaa ccatgacatg
     3061 ataacccggc ggcaaaaccg gtgacgcgga ttgcgcctgc gctggactgg atttttcggt
     3121 tttggtcatt ttaagaatcc caacatcaaa ttctttaaga acgcggctga ctttgtcgag
     3181 cggcaaacgc ggcgatgtgt ccgccggcga ccaattcatc aatggacctt agaaggtctt
     3241 ccacgtccaa aatcagcaac ggggaaccat cttccatgaa cgcgcccgcg ctgatgttcc
     3301 ggacttttcc aaggcgtggg tcgagcgcct gcacaaccaa ttcacgttcg ccaagaaaac
     3361 ggtccaccac caggccatgg cgatggtttg cgcggtcgcc cagcaccact actgaaattt
     3421 cgtcgtcatc ggcagaaatt tcgccccgct ccaaaatctg ccgcgccgtc aataaaccaa
     3481 tctgctgatt tgccagggtg aaatggggcc ggccttccaa tgtggcaatt tggttcatcg
     3541 gcaatttcaa tgcgcggctg gtttgggaaa gggggattgc ataaggctcg cccgcgattt
     3601 ccacaagcaa tgcgcgcacc acggaaagtg tcagcggcag ttgcaactga aatcgcattc
     3661 ccttgccggg ttgcgtcgcg atgcggatgt tgccgcgaac gctgcgcacc a
//
LOCUS       DASZMM010000205         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3381933, whole genome shotgun sequence.
ACCESSION   DASZMM010000205 DASZMM010000000
VERSION     DASZMM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3381933"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            757..1431
                     /locus_tag="VG754_04905"
     CDS             757..1431
                     /locus_tag="VG754_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18580.1"
                     /translation="MTKPKQSYSGVIPDGIPKSSSCGNMARACRARGFSLLELLIVMS
                     LIIIMFTMYWSSSAKNYQVAKLSQCEKNLQFIYLALRTYSTDNNDHLPSLANAQTSEP
                     VLSQLVPRSTTSTEYFTCPGCKDSPLPDGQPFANGKISYAYYMGHTLTDGADQPLLSD
                     RQVNTDPKVSGQALFSSNGKKPGANHNKYGGNVEFCDGNVQRSGPGAAFNLTNAANIV
                     LLNPKP"
     gene            1433..3916
                     /locus_tag="VG754_04910"
     CDS             1433..3916
                     /locus_tag="VG754_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF18581.1"
                     /translation="MLLTIKVQCPCGTRFAFEVLPVNGRMPVQVNCPTCNADDTEIAN
                     AQIQQQLSAQSASAAVPVAAPASVPVAVPVAIPVAVPATTPRPSPVAIPSAAPAPASS
                     SVAIPVAQTAPAPSLPTGLVIPVAQPAQLAAPTPRVPSASPPAPQPPTASGLRIAGSH
                     HAASAPVASVAVPVAVAANEAGAEAAAQLCPKHKTEPAVEKCKVCGKLICLKCMEMFG
                     YVCSVYCRQQATAKRIYVPVYANQKTVVAGKSHAMAKRITVAVVALVVLLLGFWFWYA
                     WFARDPKIVYSLPIPKVDFINNPKAIARPEEFYELIAPGQLLSIKNKQVSLFDVTEQH
                     SIWSTPLRNDSAKPKDSADAPDESDFYFGSPRVVVTTNDLWVVMSGLLARFDRQTGSP
                     KDTGIKDKILNITVRPDCILVVGGTRGAHEILANISLPDGNVQTEEISAAPKKQPVAP
                     AQPAAKVAAKKTKPNASVGKSNLPVGQLKNLAAESQPTPAPAAQADANAMPDDFEDFS
                     YRMPFTAAGPNAVEMKTKLLEQKSVSHQAMKPKGKSVLDGNVSASQGLELAQEMMNDS
                     RREETGGVEVEDVSRYQVTLHRRLQKDVPDWTGEVNGKPQLFPLKTVDLLVAGQGLMV
                     FDKQNKKLWDAKLTFPVVRHYDFLEEEPAPSLETKDALYFADAGTLTRFDLASGNVRW
                     RYNTVGITRIQADERGGIYIVTSTAGPESIQYSQQVNIHEKIKPVVMRLDAATGKVRW
                     RNEGLGDEIMLTGKFLYATRVSMTYAALRLEEGPDTHYNLNLVDPSNGSLIWNYHQGN
                     HPVMKTEVQKNWILLQMDDEVVVLKFFTF"
     gene            4007..>4195
                     /locus_tag="VG754_04915"
     CDS             4007..>4195
                     /locus_tag="VG754_04915"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HWF18582.1"
                     /translation="MGSEDLSRLALELPPDERLELARRLVESVITPSTINEAVVEGIR
                     RIEDVATGRVKGLSEQEYR"
BASE COUNT          935 a         1190 c         1120 g          950 t
ORIGIN      
        1 tgcaagaaat tagccggggg taaggccgca ggccgccacc cccggttgtc tgttgttcaa
       61 ttttccaccc tgaaagggtg gcagaaacgc gattggttcg ttggttctgg caccccttca
      121 gggtgcatcg gtttacgcga cgttttccgg gggtgtcgct tcgctcaacc cccggctaat
      181 ttcttgcaac cctccgggtt gcttcagaca gccatctcgt catcgggatg gcgccggacg
      241 aaaacctcca tttcgcgcga tcagacactg cgtgtctcgg caatcgccgg tgctccttcg
      301 gagcacaggg cacctttgcg actttgcgtt tcatcaacga attttccgcc atcgtcgcgg
      361 agaaatttcc gcttaattcc gcttgtttcc accccgttcc gcttaattcc tgataaaaaa
      421 attaattttt tcgaacgtaa actcatcgta ttaacgctcc tgcgccacgt ctttgccatc
      481 ctcaaacttt ggcaagtcac ttcgtgtcgc ttcggggaaa aagcggcaga ggactgccgc
      541 agtccaagac gctatcgcga cttccaagca tcgtcggaat tccacgaggt ctttgctttg
      601 ctatcctcta tcctcgcttt ttcaactgtg tcgcttggcg gtcaagccat ttgcctttcc
      661 agcaacactc cattactcca acactccatt actccaatgt tccccgccgg cgcaccgctt
      721 gtaatcgctt atttacgctt taaaaattat ttttgcatga caaagccaaa acagtcatat
      781 tcaggcgtga tcccggacgg cattcccaaa tcttcatcct gcggcaatat ggcccgcgcc
      841 tgccgcgcgc ggggattttc actgctcgaa ttgctcatcg tgatgtctct catcatcatc
      901 atgttcacga tgtattggag cagcagcgcg aaaaattacc aggtggcgaa gctgtctcag
      961 tgcgaaaaga accttcaatt catctatctc gccttgcgca cttattcgac ggacaacaac
     1021 gaccaccttc cttcactggc caatgcccaa acatccgaac ctgttctcag ccaattggtg
     1081 ccgcgttcca cgaccagcac cgagtatttc acctgtcccg gttgcaagga cagcccgttg
     1141 ccggacggcc aaccattcgc gaacggcaag atcagctacg cctattatat gggccacacg
     1201 ctcaccgatg gcgcggacca gccgttgctt tccgaccgtc aggtgaacac ggatcccaag
     1261 gtcagtggac aggctttgtt ttcctccaat ggcaaaaaac cgggtgcgaa ccacaacaaa
     1321 tacggcggca atgtcgagtt ctgcgatgga aacgtccaac gcagcgggcc gggcgcggcg
     1381 tttaacttga cgaatgcggc aaacattgtt ctgctgaatc ccaaacccta ggatgctctt
     1441 aaccatcaaa gttcaatgtc cgtgcggcac gcgcttcgcg tttgaagtcc tgccggtcaa
     1501 tggccggatg cctgtgcagg tcaattgccc aacctgcaac gcggacgaca ccgaaatcgc
     1561 caacgcgcag attcaacaac agctctcggc acaatcggcg tcagcggcgg tgcctgtggc
     1621 tgcgccagcg tccgttccgg ttgcggtgcc tgtggccatt ccggtcgctg ttcccgcaac
     1681 aacgcctagg ccgtcgccgg tcgcgattcc gtcagcagca cctgcgcctg cttcatcctc
     1741 agttgctatt ccagttgcac aaactgcgcc agcgccttcg cttccgactg gtttggtaat
     1801 tcccgttgcg caaccagcac agctggcagc accaactcca agagtgcctt ctgcttctcc
     1861 gcccgcgccg caaccgccta cagcttccgg tttgagaatc gccggctcgc atcatgccgc
     1921 atctgctcct gttgcttcag tcgcggttcc ggttgcggtt gcagcaaacg aggccggtgc
     1981 agaagccgcc gcccagcttt gtcccaagca caagaccgag cctgccgtgg agaagtgcaa
     2041 agtttgcggc aaactgattt gcctcaagtg catggagatg ttcggctacg tttgcagcgt
     2101 ttattgccga cagcaggcga ctgccaagcg catttacgtt ccggtctatg cgaaccaaaa
     2161 aaccgtcgtg gccggaaagt ctcacgcgat ggccaaacgc atcacggtgg cggtggtggc
     2221 gctggtcgta ttgctgctcg ggttttggtt ttggtacgcg tggttcgccc gcgacccgaa
     2281 gattgtctat tctttgccca tcccgaaagt tgatttcatc aataatccaa aggcaatcgc
     2341 aaggccggag gaattttatg agttgatcgc gcccggccag ttgctgtcga tcaaaaacaa
     2401 acaggtttcg ctctttgatg tgacggaaca acattcgatc tggtcaacgc cgttgcgcaa
     2461 tgactcggca aagcccaagg attcggcgga cgctccggac gaaagtgatt tttattttgg
     2521 aagtccgcgt gttgtcgtca ctacgaatga tttgtgggtg gtgatgtccg gcctgttggc
     2581 ccgcttcgac cgccagactg gcagccccaa ggacaccggc atcaaggaca aaatcctgaa
     2641 catcaccgtt cgtccagact gcatcctggt ggtcggaggg acgcgaggcg cgcacgaaat
     2701 tctcgcgaat atttcgctgc cggacggcaa tgtccagact gaagaaattt ccgcggcccc
     2761 aaaaaagcaa cctgttgcac cggcccagcc cgccgccaaa gttgcagcca aaaaaaccaa
     2821 gccgaacgcc tctgtcggaa agtcaaatct tcctgtgggc cagttgaaga atcttgccgc
     2881 ggaatctcag cccacgcccg ctcccgcggc acaagcagac gccaacgcga tgccggatga
     2941 ttttgaagat ttcagttatc gcatgccatt caccgccgcc gggccgaatg cggtggaaat
     3001 gaaaaccaag ttgctggaac agaaatctgt gtctcaccag gcgatgaaac ccaagggcaa
     3061 atcggttttg gacggcaacg tcagcgccag ccagggcctt gaacttgcgc aggaaatgat
     3121 gaacgattcg cgccgcgagg aaaccggcgg tgtggaagtg gaggacgtga gccgttacca
     3181 ggtcacgctt caccggcgtc tgcaaaagga cgtgcccgat tggacgggcg aagtgaacgg
     3241 caaaccgcaa ctttttccgc tgaagacggt ggatttgctt gtcgccgggc aggggttgat
     3301 ggtgttcgac aagcagaaca aaaaattgtg ggacgccaag ctgacctttc cggttgtgcg
     3361 tcattacgat ttcctggagg aggaacccgc gccaagcctg gaaacgaagg atgctttgta
     3421 ttttgcggat gcgggcactt tgacgcgctt cgatttggcg agcggaaatg tccgttggcg
     3481 ttacaacact gtcggcatca cgcggatcca agccgatgaa cgcggaggca tttacatcgt
     3541 gaccagcacc gccgggcccg agtcgataca atattcgcag caggttaaca tacatgaaaa
     3601 gatcaagccg gtcgtgatga ggctggacgc cgccacgggc aaggtgagat ggcgcaacga
     3661 aggattgggt gatgaaatca tgctcaccgg aaaattcctt tatgcgacgc gggtttcgat
     3721 gacatacgcg gctctgcgtc tcgaggaagg accggacaca cattacaact tgaatctggt
     3781 cgatccttcc aacggcagct tgatctggaa ttatcatcaa ggcaaccacc ccgtcatgaa
     3841 gacagaagtg cagaaaaact ggatcctgct gcaaatggat gacgaagtgg tggtgttgaa
     3901 attttttacg ttttgaccgc ggacattagt tccgcggttc gctctttgaa atagatggga
     3961 ttttcgcagg gcttgctttg caaccggcgc ggggtaatgt tttggcatgg gttcagaaga
     4021 tctatcgcgc ttggcactgg aattgcctcc ggacgaacgt ttggaactgg cacgtcggct
     4081 cgtcgaaagt gtcataactc cttctaccat caatgaggct gttgttgaag gcattcgacg
     4141 cattgaagac gtcgccaccg gccgcgtgaa aggtttgagt gagcaggagt atcgc
//
LOCUS       DASZMM010000206         4094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2556067, whole genome shotgun sequence.
ACCESSION   DASZMM010000206 DASZMM010000000
VERSION     DASZMM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556067"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..694)
                     /locus_tag="VG754_04920"
     CDS             complement(194..694)
                     /locus_tag="VG754_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18583.1"
                     /translation="MKHLTTSLARLFAARFQSAIRNPQPAITFANIGEGSHFSGNKSY
                     LPDVAIANRFNLVVIGSSQNNVTPSAANTDIPLGVCTDSPESTNLDVPVNVALLGCAN
                     GTLKIVLGGTVNAGDMLQSNGDGTAIKLLTTTGTFYNIGRALMSGVAGDTLEFAHTFP
                     QKVVNS"
     gene            complement(751..2139)
                     /locus_tag="VG754_04925"
     CDS             complement(751..2139)
                     /locus_tag="VG754_04925"
                     /inference="COORDINATES: protein motif:HMM:NF021627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HWF18584.1"
                     /translation="MNTPTMKPTSNIEHRTLNSQSRKPKRPSFIRRSMFDVQRSMFPQ
                     NFKVRFANSALRTSHSTFLFANDLALGHDGWAQLAPFGDFPGQAMLRNADGSITQFPA
                     IQRLDRAAADMMVAKFKSPWNRVKRYFTGCQIYAGHPDVPALANEHPDRTPKGMIVDL
                     QARANGLFCKPVFTREGSELVETRKLRAFSPYWTASEIGEQPGADGKPVKIFRPEHLN
                     SAGLTNNPNLPVQLLNEKRENIEHPISNAEHRIEDVRAPIPHSAFNVGRSAFDVQPTV
                     PALNPQHSSFNHLMNKQLILDFLATQGMTLANEATDEQIAAALQQLGERTTIAETTFA
                     SQSLELETARAELANERQFHADTLLDSALIAGRITAAQRPEWAARLQTDFANASAALA
                     QLAPTLKTTALTLNFGARKAEIANAPDRRDALDTLVKAEMAVNGGDYDRAFATVQKAN
                     PALFAAMKKPAQ"
     gene            complement(2155..3399)
                     /locus_tag="VG754_04930"
     CDS             complement(2155..3399)
                     /locus_tag="VG754_04930"
                     /inference="COORDINATES: protein motif:HMM:NF017850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HWF18585.1"
                     /translation="MNPLITRERVEQSIRARFNPVPMLEPRLLTSFLDAFRLGFFRNI
                     ALAWDVLERRDYKIQAVASKRKKAVARYGWEILTLDDSPAAQQQKLALEYFYNHVTAT
                     NVLDENETGGFALLVRQMMDAVGKRYAVHEIVWKPVASANSERGTGNPQLTATFRFCP
                     LWWFEGTQGRLRFLTSEFAIYGVEMEPGGWLVTVGDGIMEASSVAWVYKSLPLRDWIA
                     YTEKFGTPGIVGKTDASLNSKEWEDFSEIIKNFSQNWSAIVSKGNEVELIQPKGASET
                     PFATVIEKMDRAITTLWRGADLGTMSSHNAAGASLQADETNLLEVDDAQMISETLTAQ
                     VSRHVLQYHFGDAPQLAYIKIRTPDRKEVAQSLQVDQFLLQSGAPVAVKDALERYDRP
                     IPDAGEVLLKAGAPTQVAHEAK"
     gene            3861..>4094
                     /locus_tag="VG754_04935"
     CDS             3861..>4094
                     /locus_tag="VG754_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18586.1"
                     /translation="MPPAGVVTGYGKGNGNAHGVPFLASNSRPTLHDVSVMFPLSHAG
                     ARKMKPTVGLTAFFLKINLCEKKPVSINASAGFS"
BASE COUNT          833 a         1065 c         1258 g          938 t
ORIGIN      
        1 ttcgctgaaa tacgtttcga ggaaacgcga atcattggca aacagcacct ggccgggttc
       61 ataaccggaa ccgcgatcag gcagggcgat ggggatggga ttcatgattt ttgattttcg
      121 attttagatt tttgattggg gactggaatg gatgggcgga atggagcaat agctgaccat
      181 caaccatcaa ctatcaacta ttcacaactt tctgtggaaa cgtgtgcgcg aactcgaggg
      241 tgtcgccggc gacgccggac atcaacgcgc ggccaatgtt gtaaaacgtg ccggtggtgg
      301 tgagcagctt gatggcggtg ccgtcgccgt tggactggag catgtcgccg gcattgaccg
      361 tgccgccgag cacgattttc aatgtgccgt tcgcgcaacc gagcagcgcg acgttcaccg
      421 gcacatccag gttcgtggat tcaggcgagt cggtgcaaac gccgagcgga atgtccgtgt
      481 tggcggcgct gggcgtgacg ttgttctgcg aactgccgat gacgacgaga ttgaaccggt
      541 tcgcgatggc aacatccggc agatacgatt tgttgccgga aaaatgcgag ccttcgccga
      601 tgttggcgaa agtaatcgcc ggttgcggat tgcggattgc ggattgaaac cttgcggcga
      661 ataagcgcgc gagggaagtg gtgaggtgtt tcattttatt attgcgtgtt tcggatttgg
      721 gatttggatt ttctttgggt gtttgggtgt ttattgcgcc ggttttttca tcgcggcgaa
      781 caacgccgga ttcgcctttt gcaccgtggc gaatgcgcgg tcgtaatcgc cgccgttcac
      841 ggccatctca gccttgacca gcgtgtcgag cgcatcgcgc cggtcgggtg cgttggcgat
      901 ctcggccttg cgcgcgccga agttcagcgt gagcgcggtg gttttcagcg tgggggcgag
      961 ttgcgccaaa gcggccgagg cattggcgaa atcagtttgc aaacgcgcgg cccattcggg
     1021 gcgttgcgcg gcggtgatgc ggcccgcgat gagcgcggaa tcgagcaacg tgtcggcatg
     1081 gaactgccgc tcgttcgcca attccgctcg cgccgtttcc agttcaaggc tctggctggc
     1141 gaatgtggtt tcggcgatgg tcgtgcgctc gccaagttgc tgcaacgcgg cggcgatttg
     1201 ctcgtcggtg gcctcgttgg caagcgtcat tccctgcgtc gcaaggaaat cgaggatgag
     1261 ttgtttgttc attaaatggt tgaaggatga gtgttgaggg ttgagggcag ggacggtggg
     1321 ttgaacatcg aacgctgaac gcccaacatt gaacgctgaa tgggggatgg gcgcgcgaac
     1381 gtcttcgatt cgatgttcag cgttcgatat tggatgttcg atgttttctc ttttttcatt
     1441 cagcagttgc accggcagat tcggattatt ggtaagcccg gcggaattga gatgttcagg
     1501 tcgaaaaatt ttcaccggct tgccatcagc gccaggttgc tcgccgattt cgctcgccgt
     1561 ccaatacgga ctgaacgcgc gcaactttcg cgtctcgacc aactcgctgc cttcgcgggt
     1621 gaaaaccggc ttgcaaaaaa gtccgttcgc gcgcgcctgc aaatccacga tcattccttt
     1681 cggcgttctg tcgggatgct cattcgccaa cgccggcacg tcgggatgac ccgcgtaaat
     1741 ctggcagccg gtgaaataac gcttcacgcg gttccacggc gacttgaatt tggcaaccat
     1801 catatccgcg gcggcgcggt cgagccgttg gatcgcggga aattgtgtga tggaaccatc
     1861 ggcattgcgg agcatcgcct ggccgggaaa atcgccgaac ggcgcaagct gcgcccagcc
     1921 gtcgtggccc agcgcgagat cattcgcgaa aagaaatgtg gagtgcgagg tgcggagtgc
     1981 ggagttggcg aagcgaactt taaaattttg cggaaacatc gaacgctgaa catcgaacat
     2041 cgaacgccga atgaaagaag ggcgtttcgg cttccttgat tgggagttca gcgttcgatg
     2101 ttcgatgttc gatgttggtt tcatggttgg cgtgttcatg actcaaaatt gaacttactt
     2161 tgcttcgtgc gcgacctgcg ttggtgcacc cgccttcaac aacacttcgc cggcatcggg
     2221 aatcggacgg tcgtagcgtt cgagcgcgtc cttcaccgcg acgggcgcgc cggattgcaa
     2281 aaggaactgg tccacttgca gcgattgcgc gacttccttc cggtcgggcg tgcggatctt
     2341 gatgtaggca agctgcggcg cgtcgccgaa atgatattgc aaaacgtgac ggctcacttg
     2401 cgcggtcagc gtttcggaaa tcatctgcgc gtcatcgact tccagcagat tcgtttcatc
     2461 cgcctggaga ctcgcgccgg cggcgttgtg cgaactcatc gtgccaaggt ccgccccgcg
     2521 ccagagcgtg gtgatcgcgc ggtccatctt ttcgatgacc gtcgcaaaag gcgtttcgct
     2581 cgcgcctttc ggctggatca attccacctc gttgcccttg ctgacaatgg cggaccaatt
     2641 ctgcgaaaaa ttcttgatga tctcggagaa atcctcccat tccttcgaat tgagcgacgc
     2701 gtccgtcttg ccgacgatgc ccggcgtgcc gaatttttcc gtgtatgcga tccagtcgcg
     2761 caacggcaaa cttttgtaaa cccacgcgac cgaactcgcc tccatgatgc cgtcgccgac
     2821 cgtcaccagc catccgcccg gttccatctc gacgccgtaa atggcaaatt cgctcgtgag
     2881 aaaacgcaac cgtccctgcg tcccttcgaa ccaccaaagc ggacaaaagc ggaaggtggc
     2941 cgtgagttgc gggttgccag ttccgcgttc cgagttggcg gaggcaaccg gtttccaaac
     3001 gatttcatgg acagcgtaac gtttcccaac cgcgtccatc atctgccgca ccagcaatgc
     3061 gaaaccgccg gtttcatttt cgtcgagcac gttggtcgcg gtgacgtgat tgtagaaata
     3121 ttccagcgcg agcttttgct gttgcgcggc gggcgaatca tcgagcgtga ggatttccca
     3181 gccgtaacgc gcgacggctt tcttgcgctt ggacgcgacc gcctggattt tgtaatcgcg
     3241 gcgctccagc acgtcccacg caagcgcgat gttgcgaaaa aacccgagcc ggaaggcatc
     3301 aagaaaagac gtgagcaacc gcggctctag catgggaaca ggattgaatc gcgcgcggat
     3361 gctttgttcg actcgttcgc gtgtgataag tggattcatg ggattaaaaa tttaaagggc
     3421 aaagtttaag gaacatcgaa catccaacac cgaacgctga acgttgaatt gaacgcgccg
     3481 tagcgccttt cttgcattca gcgttggaag ttcgatgttc agcgttcgat gttttggctc
     3541 gctgagggga gaaggagcgg cgcaaggaca ggattgcgct cagtcgcggc gcccgggagg
     3601 acgggcggca aggcgaccaa ccatctcctt ctccccaaga atggttgaag gttgagggtt
     3661 gagggttgag ggagaaaaat tcccgaacgg cgcgctgcgc cgaaccgttt ggtgtccatg
     3721 ctttagcatg ttctttctgt ttcggacttg gagacacgct aaagcgtgga cacccaacaa
     3781 aattagaaga cgccggacgg tgcgacctga gatgcgtggg agcggtcagg tgggaagacc
     3841 gggaatgggc ccaggtggga atgccgcccg ccggcgtcgt gaccggatac gggaagggga
     3901 atgggaacgc gcatggcgtg ccgtttttgg cgtccaactc ccgaccaact ctgcatgacg
     3961 tatcagtcat gttcccgtta tctcatgcgg gtgcccgaaa aatgaaacct accgtaggtt
     4021 tgacagcatt ttttttaaaa attaatcttt gtgaaaaaaa gccggtttcg atcaatgcct
     4081 ctgcgggttt ctcg
//
LOCUS       DASZMM010000207         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_367361, whole genome shotgun sequence.
ACCESSION   DASZMM010000207 DASZMM010000000
VERSION     DASZMM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367361"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1476)
                     /locus_tag="VG754_04940"
     CDS             complement(292..1476)
                     /locus_tag="VG754_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWF18587.1"
                     /translation="MSTNIWPLNPEIIFLNHGSFGCCPRPVLEFQQSLRERMERQPVQ
                     FFVRDLEPLLDEARNAVARFVGANADNLVFVQNATSGVNTVLRSLRFEPGDELLVTDH
                     EYNACRNALEFVANRWGAKVVIATVPFPLGSADEILYAILSHVTERTRLALLDHVTSQ
                     TALILPISQLVERLNARGIDTLVDGAHAPGMVPLNLESLGAAYYTGNCHKWICAPKTA
                     GILHVRANRQHLIHPLAISHGLTSPRKDRSKFLIEFGWMGTGDPTACLSVGEALRFVG
                     SLVPGGWPEVMAKNHSLALAGRKLLCDALQIPPPCPDELIGSMASVPIPDARAEEKSI
                     SPLYLDPLQDELLRDHNFEMPVIPWPAPPKRLIRISCQLYNSLPQYQTLAGALKMKFG
                     GL"
     gene            complement(1606..2682)
                     /locus_tag="VG754_04945"
     CDS             complement(1606..2682)
                     /locus_tag="VG754_04945"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWF18588.1"
                     /translation="MFPTQDLHVKELVRLSTPSALKAELPTTEASNVTVVRGREAITR
                     ILRQEDPRLLVIVGPCSIHDPAGALEYAKKLNALRQEFAGQMEIVMRVYFEKPRTTIG
                     WKGLINDPFLDNTHDIEAGLKKARQLLLEITGMGLPAATEFLDPIIPQYIADLISWAA
                     IGARTTESQTHREMASGLSMPVGFKNGTDGSLQTAIDAMGAAMRPHSFLGIDQDGFTS
                     IVRTTGNTTGHVVLRGGRLQTNYDAESIREAEMKLKQAGLRPGLMVDCSHANSGKQHA
                     RQEDVWRSVIEQRIAGSPSLTGVMIESYLREGSQPFPKNPAELQYGISITDACLGWEV
                     TERMLRWGNAELQKARVQPATAMA"
     gene            complement(3202..>3789)
                     /locus_tag="VG754_04950"
     CDS             complement(3202..>3789)
                     /locus_tag="VG754_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18589.1"
                     /translation="GLFINGKALLIDRTYRWFGVPHQEYRFYNDLEVVGIYLYQSSHI
                     ETVKIATKIIPDSAEARFNLAYRLAENGKLKEARAALAEGLKLDSQNWHGLMVLAFIE
                     GQQDNWDGTVNHLEMVLQKNPDYNLARYFLGTAWYHHGSMNEARDEYRKYLQGETEPP
                     YAEYAQKAIIQINEWTQGTNTESNAKANPSTPIKQ"
BASE COUNT          861 a         1086 c         1014 g          828 t
ORIGIN      
        1 gggattgacc gtgacgaaca ggacatccgg cggcggggca ggaggagcgg gttttttgca
       61 gccggctgcg gcaagcaaaa ggagaacaat ggcgagcaac cctgcgcgcc cacttttcgc
      121 catcataaaa ttcagcatag cccttattcg ctgcctatta tagctcacct ttgccaaatg
      181 caaaatgcag tgtgaatgat tcgaagatgc gaacaagggc gctgttcgcg aacggagcgg
      241 tttgatggtc tttcgtcatt tcccattatg agtccgtcac cctcgaaaaa gttacaaccc
      301 gccaaatttc atttttaacg ccccggccaa tgtttgatat tgtggaagcg aattataaag
      361 ctggcacgaa atgcgaatga gccgtttcgg cggcgcaggc cacggaatca ccggcatttc
      421 aaaattgtgg tcgcgcaaca gttcgtcctg caacgggtca aggtaaagcg gcgaaattga
      481 tttttcctcc gctcgcgcgt ccgggatggg aaccgaagcc atcgagccaa tgagttcgtc
      541 agggcagggc gggggaatct gcaacgcatc gcaaagcaat tttcttccgg ccagtgcaag
      601 cgaatgattc ttcgccatga cttccggcca gccgcccgga accagtgaac cgacaaatcg
      661 cagcgcttca ccaacgctca ggcaagcggt cggatcgccc gtgcccatcc agccaaattc
      721 aatcaggaat ttcgagcggt ccttgcgcgg cgaagtcagg ccgtggctga tggccagcgg
      781 atggatcaaa tgctgccggt tcgcgcggac gtgcagaatt ccggctgtct tgggcgcgca
      841 aatccattta tgacagttgc ccgtgtaata agccgcgccg agcgactcca gattcagcgg
      901 caccatgccg ggcgcgtgcg cgccatccac cagcgtgtca atcccgcgcg cgttgagtcg
      961 ctccaccaat tgcgaaatcg gcaggatcaa cgccgtctgg ctcgtgacgt ggtccagcaa
     1021 cgcgagacgc gtccgctcag tgacgtggga aagtatcgcg tacaagattt catcagccga
     1081 gcccaaggga aacggaacag tggcgatgac cactttcgcg ccccagcgat tcgcaacgaa
     1141 ttccagcgca ttccggcagg cgttgtattc atggtccgtc accagaagct catcgccggg
     1201 ttcaaaacgc aacgagcgca acaccgtgtt cacgccgctg gtggcgttct ggacgaaaac
     1261 caggttgtcc gcgttcgcgc caacgaaccg cgccaccgcg tttcgcgcct catccagcaa
     1321 cggctccaga tcacgcacga aaaattgcac cggctgccgt tccatccgct cgcgcaacga
     1381 ttgttgaaac tccaacaccg gccgcggaca acatccgaac gagccgtggt tcaggaaaat
     1441 gatctccggg ttgagcggcc agatgttagt gctcatgtga aatgaaaagc tccaagcgcc
     1501 aacatccagg caccagagaa gctccaaact tcaaacacca aatcaagagg ccgcggaatt
     1561 ggagcttgct gcttggacgc ttggggcttt gtttaggcat cttgcttaag ccatcgccgt
     1621 cgcaggttga acacgcgcct tctgcaactc cgcgtttccc cagcgcaaca tccgttccgt
     1681 cacctcccag ccgaggcagg cgtccgtgat ggatatgccg tattgcaatt cggcgggatt
     1741 tttcgggaac ggctggctgc cttctcgcag ataactctcg atcatcacgc ccgtcaacga
     1801 aggcgaaccg gcgatgcgct gctcgatgac actgcgccag acatcctcct gacgcgcgtg
     1861 ttgcttgccc gaattcgcgt ggctgcaatc caccatcaag cccggacgca accccgcctg
     1921 cttcaatttc atctccgcct cgcggatgct ttcggcgtca taattcgtct gcaaccgccc
     1981 gccgcgcaac accacatgcc ccgtcgtgtt gcccgtcgtg cgcacgatgc ttgtgaagcc
     2041 gtcctggtca atgccgagaa aactgtgcgg acgcatcgcc gcgcccatcg cgtcaatcgc
     2101 cgtttgcagg gaaccgtccg tgccgttctt gaaaccgacc ggcatcgaaa gtccgctcgc
     2161 catctcgcga tgcgtttgcg attcggtcgt gcgcgcgccg atcgccgccc aactgataag
     2221 gtcggcgatg tattgtggga tgatcgggtc aagaaattcc gtcgccgctg gcaaacccat
     2281 gccggttatt tccaacaata actggcgcgc ctttttcaat ccggcttcga tgtcgtgcgt
     2341 gttatcgagg aacgggtcgt tgatgagccc tttccagccg atggtcgtgc gcggcttttc
     2401 aaaatacacg cgcatcacga tttccatctg gccggcgaat tcctgccgca acgcattgag
     2461 cttcttggca tattcgagcg cgcccgccgg atcatggatc gagcaggggc ccacaatcac
     2521 gagcaaccgc gggtcttcct gccgaagaat gcgcgtgatg gcctcacgcc cgcgaaccac
     2581 ggtgacattg gacgcctcgg tcgtcggcaa ttcggccttc aacgcgcttg gcgtcgagag
     2641 ccgcaccaat tctttgacat gtaaatcttg agtcggaaac atcccggcca ctatatcaga
     2701 tgaggaagct gaggaaagcc gaaatcacgg tgagtggacg cgcgattggt gtttggtagg
     2761 gacggcccgc tgggccgtcc gcagacgccg caacgtggcg tccctcccat ttatgaggtg
     2821 aattgtaact tgagacgaaa tcctaaaact gttaactctt tccaatgcgg actgcttctc
     2881 aaaatcaaaa gcacaagacc aaaaaagctt tgaatgccaa tccaaatcag cacaaatggg
     2941 atgacgtaat gggggaagtc tggcaggcgc aaaaacggat caaaccatcg gaacgcacac
     3001 ctaacccggt gcttgaagag cgcgcgcgtc ggcggcgatg aaaaccccaa cggggttttg
     3061 gaaattagcc cagggttggt ccgagtcagc cgagggccta ccctgggtat gctgtcaaaa
     3121 cgaaaccaac actttcgagc gaagcgaggc gagcaacgcg agcatcaatt gagcgcgcac
     3181 tgatactttc tttttcaaaa cttactgttt tatcggtgtt gatggatttg ctttggcatt
     3241 gctttccgta ttcgtgcctt gcgtccattc atttatttgg atgatggctt tttgggcata
     3301 ttccgcatag gggggttcag tctcgccttg gaggtacttg cgatattcat cgcgagcttc
     3361 gttcattgat ccatggtggt accaggcagt gcccaaaaaa tagcgggcga ggttatagtc
     3421 gggatttttt tgaagaacca tttccagatg gttcacggtt ccgtcccaat tgtcttgctg
     3481 accttcaatg aaagctagta ccataagccc atgccagttt tgtgaatcca atttcaatcc
     3541 ttcggccagg gctgcccgtg cttctttcag tttgccattc tcggccaaac ggtatgccag
     3601 attaaaacga gcctctgcag aatcgggtat gatcttcgtt gcgattttca cagtttcaat
     3661 atgagacgat tgatacaaat aaatgccgac cacctccaag tcgttgtaaa aacgatattc
     3721 ttgatgcggc acgccaaacc accggtatgt gcgatctatc agaagcgcct ttccattgat
     3781 gaacaaccc
//
LOCUS       DASZMM010000208         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2836404, whole genome shotgun sequence.
ACCESSION   DASZMM010000208 DASZMM010000000
VERSION     DASZMM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836404"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VG754_04955"
     CDS             <1..699
                     /locus_tag="VG754_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18590.1"
                     /translation="LKHGHQTAFEAPSVSANPIVRCPRFSVHRHNRREHAKAWTPNDP
                     YRGVQLSSFLCALVSLRLCVSSTYFPPSSRRNFRLIPLVSTPFRLIPDKKINFFERKL
                     IVLALLRRVFAIRYPLWGVSLQGKAAEDCRTPRRYRDFQASSEFREVFAFAILYLLSS
                     ILTFSTVSLWRSIRSLFHHANQDLGIRSQAINPFAFPASKFLYETVCFHGVVLPAVFQ
                     LRLNKHGLTCVLIP"
     gene            711..2282
                     /locus_tag="VG754_04960"
     CDS             711..2282
                     /locus_tag="VG754_04960"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWF18591.1"
                     /translation="MLQRIFGVAARGRFLTAVTILILQAVVLTPLRAQQVPVIEKTLD
                     NGMRLLMVERHDEPSIAGGWVAHVGSANERPGITGIAHLFEHMMFKGTKTIGTKDYEK
                     DLEILAEQERVRDEMRKEEAKMRVEFRKGEIDDLMKPENKTPRWRELNKQFEKLVNDE
                     RQILVKNEFDQIYSKEGASGMNAFTMEDMTAYFVGVPANKLELWMWMESERINHPVFR
                     EFYAERDVVFEERRMRTDSTPLGKFEEEAEAITWESVPYHWPVIGWPSDISSISKAQA
                     DDFYAAYYTPQNITLILVGDFKPEEAEALAKKYFERIPRGKKDPPDVITTEVPQLAEK
                     RMYAEAEVNPQIDVEWHTVPFGHKDSYALRVLAELLSTRTGRLYKGLVLGSEVATSVD
                     ATQDSRKWAGLFDISGEAKDGKTPEDVEQGIYAQLEKLKKEPAPAEELQKVKNEFAAN
                     EYRKLTANMPILHLLIMNDGEGDWHEINNADVKIQAVTAEDIERVAKKYFTKENRVVS
                     VFTRKGSSPKPEAVQ"
     gene            2411..>3324
                     /locus_tag="VG754_04965"
     CDS             2411..>3324
                     /locus_tag="VG754_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWF18592.1"
                     /translation="MLSILFLVGAGIGTSKASDDAVIVKRPEELKFPPLKYEPPSPAN
                     YRVALKSGPVAYVIPDKTLPLVNIAVYVRAGSYLEPAGKEGLAGLTGYLLARGGTKSL
                     PAEALEERLAFLAAKLESGVGGTQGAVSLNLLSKDLDEGLGILREVLTEPRFQDDKIA
                     LRKEQLIQDMKQRNDQSEDIEAREYGFLAHGENFWENHYPTQASVESITREDLENFHH
                     KWFHPDQFVVAVSGDFDRDEMVRKLEKLFGDWPFQGEKPPPIPTDTMLAKAGVYIVDK
                     DVNQGRVTIQLPGIKREDPDYFPVQVMN"
BASE COUNT          796 a          859 c          931 g          738 t
ORIGIN      
        1 ctaaagcatg gacaccaaac agccttcgaa gcgccgagcg tttcagctaa tccgattgtt
       61 cggtgtccac gctttagcgt gcatcggcac aaccgcaggg aacatgctaa agcatggaca
      121 cccaacgacc cttatcgggg agttcaactg tcttcttttc tttgcgccct cgtgtctctg
      181 cgcctttgcg tttcatcaac gtattttccg ccatcatcgc ggagaaattt ccgcttaatt
      241 ccgcttgttt ccaccccgtt ccgcttaatt cctgataaaa aaattaattt tttcgaacgt
      301 aaactcatcg tattagcgct cctgcgccgc gtctttgcca tccgctatcc tctttggggt
      361 gtgtcgcttc agggaaaagc ggcagaggac tgccgcactc caagacgcta tcgcgacttc
      421 caagcatcgt cggagttccg cgaggttttt gcctttgcca tcctctacct tctatcctct
      481 atcctcactt tttcgactgt gtcgctttgg cgatcaatac ggtcgctgtt ccatcacgcg
      541 aatcaagact tgggcatccg cagtcaagcc atcaatccat ttgcctttcc agcatcaaaa
      601 tttttatacg aaacggtatg cttccatggc gtagtcctgc ccgccgtttt ccagttgcgc
      661 ttgaacaagc atggattaac ctgtgtctta attccgtgat tattctcaat atgttacagc
      721 gaattttcgg ggttgccgca cggggaagat tcctgacggc ggtcaccatt cttattttac
      781 aggcggtcgt tttgacgccg cttcgggcac agcaagtgcc tgtcatcgaa aaaaccctcg
      841 acaacggcat gaggttgctg atggtcgagc ggcacgacga accgtccatc gcgggcggat
      901 gggtcgcgca tgtcggttcg gcgaatgaac gtcccggcat caccggcatc gcgcatcttt
      961 ttgaacacat gatgttcaag ggcaccaaaa ccatcggcac gaaggattat gaaaaggacc
     1021 tcgaaatcct tgccgagcag gaacgcgtgc gcgacgagat gcgcaaggaa gaagcaaaga
     1081 tgcgcgtgga atttcgcaag ggcgagatcg acgatttgat gaagccggag aacaaaacgc
     1141 cgcgctggcg cgagttgaac aagcagttcg aaaaactcgt caacgacgag cgccaaatcc
     1201 tggtgaagaa tgagttcgac cagatttaca gcaaggaagg cgcttcgggc atgaacgctt
     1261 tcaccatgga ggacatgacg gcttatttcg tcggtgtgcc ggcaaacaaa ttggaactct
     1321 ggatgtggat ggaatcggag cgcatcaatc atccggtctt ccgcgaattt tatgccgaac
     1381 gcgacgtggt gttcgaggaa cgccggatgc gcacggattc gacgcccttg ggcaagttcg
     1441 aggaggaagc cgaggcgatc acatgggaat cggtgccgta tcattggccg gtgatcggct
     1501 ggccgtcgga catttcgtcc atctccaagg cgcaggcgga tgatttttat gccgcttatt
     1561 acacgccgca gaacatcacg ttgattttgg tcggcgattt caaaccggaa gaagcagaag
     1621 cgctggcgaa gaaatatttc gagcgcatcc cgcgcggcaa gaaagacccg ccggacgtga
     1681 taacgaccga agtgccgcaa ctggccgaga agcggatgta tgccgaggcg gaggtcaatc
     1741 cgcagatcga cgtggaatgg cacacggttc cgttcggcca caaggattct tacgcgttgc
     1801 gcgtgctggc ggagctgctt tcgacccgca ccgggcggct ttacaagggg cttgtgctcg
     1861 gttcggaagt cgcgacgagc gttgacgcca cgcaggattc gcgcaaatgg gccggattgt
     1921 tcgacatcag cggcgaggcc aaggacggca agacgccgga agacgtggag caggggattt
     1981 acgcacaact tgaaaaatta aaaaaggaac cggcaccggc ggaggaattg caaaaggtga
     2041 agaatgaatt cgccgccaat gaatatcgca agctcaccgc caacatgcct attctgcatc
     2101 tgctcatcat gaacgatggc gagggcgact ggcacgagat caataatgcc gatgtcaaaa
     2161 tacaggcggt cacggccgag gacatcgaac gagtggcaaa aaaatatttt acgaaagaga
     2221 accgggttgt gagtgttttc acgcgcaaag gaagctcgcc caaaccggag gcggtgcaat
     2281 gagcttctat ttcaatattg cttctttgat aaccgagatg aattccaaaa ctaagcggat
     2341 ggttgcttcg aacagaccag tcagtgccca cactcgccat catgttactc gatcaactgt
     2401 ttgggctgcc atgctctcga ttttgttttt ggtcggggca ggcattggga cctccaaagc
     2461 ttctgatgac gcagtcatcg tcaaacggcc ggaagaactc aaatttccgc cgttgaaata
     2521 tgagccgcca tcgcctgcca attatcgagt ggcgctgaaa agcggcccgg tggcttatgt
     2581 cattccggat aaaacgctgc cgttggtgaa catcgcggtt tatgtccgcg caggctcgta
     2641 tctcgaaccg gctggcaagg aaggcttggc gggattgacc ggttatttgc tggcgcgagg
     2701 cgggacgaaa tcacttccgg ctgaagcgct ggaagagcga ttggcatttc tggcggcaaa
     2761 attggagtcg ggtgtcggcg gcacgcaggg cgctgtcagc ctcaatcttc tttcgaaaga
     2821 tttggacgaa ggcttgggaa tcctgcgcga ggtgctgacc gagccgcgtt ttcaggacga
     2881 caagattgcg ttgcgcaaag agcagttgat tcaggacatg aaacagcgca acgaccaatc
     2941 cgaggacatc gaggcgcgcg aatacgggtt tctggcgcac ggcgaaaatt tctgggagaa
     3001 ccattatccc acgcaggcat ccgtcgagag catcacgcgc gaagacctgg agaatttcca
     3061 tcacaaatgg tttcatccgg atcaattcgt cgttgccgta agcggggatt ttgaccgtga
     3121 cgagatggtg cgcaaattgg aaaaactctt cggcgactgg cctttccaag gtgagaagcc
     3181 gccgccgatt cccactgaca ccatgttggc caaagccggc gtttacatcg tcgataagga
     3241 cgtgaaccag ggtcgcgtga ccatccaatt gccgggcatc aagcgcgaag acccggatta
     3301 ttttccggtg caggtgatga atga
//
LOCUS       DASZMM010000209         3650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5022178, whole genome shotgun sequence.
ACCESSION   DASZMM010000209 DASZMM010000000
VERSION     DASZMM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5022178"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VG754_04970"
     CDS             complement(<1..660)
                     /locus_tag="VG754_04970"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWF18593.1"
                     /translation="MNRKQFGILIVLLIVLGGAGWLVQQSRNRAAGAGEQGSGRKLLG
                     DSFPMNDVAHISIKQGSNEVNLVKKDDLWRVGERKNYPANFSEISSFLVKAADLKVVQ
                     SEDVGASQLPRLQLAVGQGTNSGTVVDLKNKDEKSIKTLTLGKKLMGKSQRPQQLPFG
                     EGEFPEGRYVMVSGNEKQALLIADPLSSVEPKPEEWLDKDFFKIERPKAVAVAYPGTQ
                     GT"
     gene            complement(657..2555)
                     /locus_tag="VG754_04975"
     CDS             complement(657..2555)
                     /locus_tag="VG754_04975"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HWF18594.1"
                     /translation="MKKNTYETILYSTAGVVILLIIIIAFNALTSTVKQRVDLTQEKA
                     YTLSAGTKAILAKLDTPVKIRFYYSSSETGSGDTVFFKSYAQHVADLLDEYKQNAHGK
                     IILEQYDPKPDSDAEDSARLDGVEGQMLRTGEKFYMGLAVSMLDSKEAIPFLDPGRER
                     LLEYDLTRAISRVGTPEKPVIGVMTPLPVFGRPGNPMLAQMGQGGQGQQPWMFINELK
                     GDYNVKQIEMTATKIEDDVKLLVVIHPKDISDGAQYAIDQFIMRGGKVIAFLDATSLV
                     DSHNDNPMMGAMPGGGSSLDKLLKAWGIQFENSKVVADMNYKVRMMRNNQPSEVPTFL
                     DITDDGINKDDIATSEIKDLWYPFGGMFTGTPMSGLKETVLVKSTKDSSLVDGTMASF
                     NPDSVLKDFKPSGTQYTLALRLTGKFKTAFPDGKPKDKDETNAAPVETTLKESKGDGA
                     VVLFGDADMLADEFSLRQQQTVFGPMGWEPANGNLLLVQNLVDQLSGDNDLISIRSRA
                     AMDRPFTRIKAMEAKAQESYQTKIDAFEQSLQDTQQKLNELQTKKGDGQQRFILSPEQ
                     QVEVEKLKKQEADVRVQLKQERKKLTHDISALENTLKWTNIIAMPAVVALSGIGLALV
                     KRKRTSAK"
     gene            complement(2571..3320)
                     /locus_tag="VG754_04980"
     CDS             complement(2571..3320)
                     /locus_tag="VG754_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF18595.1"
                     /translation="MNSWANIKTIAKRELIGYFASPVAFVFIVIFLLLTGFFTFMLGG
                     FFEQGQASLRSFFTWHPWLYLFLVPAAGMRLWSEERRQGTMELLLTMPITPSQAIVGK
                     FLASWLFLAIALVLTFPIAVTVNYLGSPDNGVILTSYIGSFFLAGAYLAVSCMTSAMT
                     RNQVVSFIISVVICFLLILVGFSPVSKFLYEVMGFHQWFVDLVASFSVMTHFENFQRG
                     VLDTRDLLFFLSVMVFSLFTTGVIIRGHRAG"
     gene            complement(3317..>3650)
                     /locus_tag="VG754_04985"
     CDS             complement(3317..>3650)
                     /locus_tag="VG754_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF18596.1"
                     /translation="TPQQLRQKSEWAGAVTLRVSGVTASALSQKLSQLSTVKKTMILK
                     EDATSVTARIFPKTGGVNTTPSGALAQSISDAAHGWRIEELHTEEGRLDEVFRNITMP
                     DTLKEEKK"
BASE COUNT          749 a         1070 c          995 g          836 t
ORIGIN      
        1 tgtcccttgc gtgcccggat acgcgacggc cacggccttg ggcctttcaa tcttgaagaa
       61 atccttgtcc aaccattcct ccggcttcgg ctcgacgctg ctcaacgggt ccgcgatcaa
      121 caaagcctgc ttttcgtttc cgctcaccat cacgtaacgg ccttcgggaa attcgccttc
      181 gccgaacggc agttgctgcg gccgctgcga tttgcccatg agcttcttgc cgagcgtgag
      241 cgtcttgatg gacttctcgt ccttgttttt caaatccacg accgtgccgg aattcgtccc
      301 ctgcccgacc gcaagttgca ggcgcggcaa ttgcgacgcg ccgacatcct cgctctgcac
      361 gaccttcaag tccgcggcct tcacgagaaa actgctgatc tcggaaaaat tggccgggta
      421 attcttgcgc tcgcccacgc gccagaggtc atcctttttg accaaattaa cttcattcga
      481 gccctgcttg atgctgatgt gggcgacgtc attcatcggg aacgaatcgc cgagcaattt
      541 cctgcccgag ccttgttcac ccgcgcctgc cgcgcgattg cgcgattgct gcacgagcca
      601 gccggccccg ccgaggacga tcaacagaac gatgaggatt ccaaattgtt tccggttcat
      661 tttgcagacg tgcgtttacg tttgacgagg gccaggccaa ttcccgacaa cgccaccacg
      721 gcgggcattg cgatgatgtt ggtccatttg agcgtgttct ccagcgcgct gatgtcatgc
      781 gtgagcttct tgcgctcctg cttcaactgc acccgcacgt cggcctcttg tttcttgagc
      841 ttttccactt caacctgctg ttcgggcgaa aggatgaacc gctgctggcc gtcgcctttc
      901 ttggtttgca attcgttcag cttttgctgc gtgtcctgaa ggctctgctc gaacgcgtca
      961 atcttggttt gataactttc ctgcgccttc gcttccatcg ccttgatgcg ggtgaaagga
     1021 cggtccatcg cggcgcggct gcggatgctg atgagatcgt tgtcaccgct gagctggtcc
     1081 accaaattct gcacgagcaa caaattgccg ttcgcaggct cccagcccat cgggccgaaa
     1141 acggtctgct gttgccgcag cgaaaattcg tcggcgagca tgtcggcatc gccgaagagc
     1201 accaccgcgc cgtcgccctt ggattccttg agcgttgttt caacgggcgc ggcgttggtt
     1261 tcatccttgt ccttcggctt gccgtcgggg aaagcggtct tgaacttgcc ggtcaaccgc
     1321 agcgccagtg tgtattgagt gcccgacggt ttgaagtcct tcaagacgct gtcaggatta
     1381 aaactcgcca tcgtcccatc caccagcgac gaatctttcg tggatttcac cagcaccgtt
     1441 tccttcaacc cgctcatcgg cgtgcccgtg aacatcccgc caaacggata ccaaaggtcc
     1501 ttgatctcgc tcgtggcgat gtcgtccttg ttgatgccgt catcggtgat gtcgaggaaa
     1561 gtcggcactt ccgacggctg gttgttgcgc atcatgcgca ccttgtaatt catgtcggcg
     1621 acaaccttgc tgttctcgaa ttggatgccc cacgccttga gcagcttgtc caaactcgaa
     1681 ccgccgcccg gcatcgcgcc catcatggga ttgtcattgt ggctgtccac gagcgaagtc
     1741 gcgtccagga atgcgatcac ctttccgccg cgcatgatga actggtcaat cgcgtattgc
     1801 gccccgtccg aaatgtcctt gggatggatg accacgagca gcttgacgtc atcttcaatc
     1861 ttggtcgcgg tcatttcgat ttgcttcaca ttgtaatcgc ccttgagttc gttgatgaac
     1921 atccagggtt gctggccttg gccgccctgt cccatctgcg caagcatcgg attgcccgga
     1981 cgaccgaaca ccggcagcgg agtcatcacg ccgatgaccg gcttttccgg tgtgccaacg
     2041 cgggaaatcg cgcgcgtcaa atcatattcc aacaaccgtt cgcggcccgg atcaaggaac
     2101 ggaatcgctt ccttgctgtc gagcatgctg accgcaaggc ccatgtaaaa tttttcgccg
     2161 gtgcgcaaca tctggccttc cacgccgtcg agccgcgctg aatcttcggc gtcggaatca
     2221 ggcttgggat cgtattgttc caaaataatt ttgccgtgcg cattttgttt atattcgtcc
     2281 agcaaatcag cgacgtgctg ggcgtaagac ttgaagaata cggtatcgcc ggagccggtc
     2341 tcgctgcttg aataatagaa acggattttc accggcgtat cgagcttggc aaggatggcc
     2401 ttcgtcccgg cagagagcgt ataggccttt tcctgcgtca aatcaacccg ctgcttgacc
     2461 gtgctggtga gcgcgttgaa ggcaatgatg atgatcagca ggatgacgac acccgccgtc
     2521 gagtaaagga tggtttcgta agtattcttc ttcatcgcaa aataaaatta tcagccggcg
     2581 cggtgtccgc ggatgatcac cccggtggtg aacagcgaaa acaccatcac cgagaggaaa
     2641 aagagcaggt cgcgggtgtc aagcacgccg cgttggaaat tttcgaagtg ggtcatcacg
     2701 ctgaacgaag ccacaaggtc aacgaaccat tgatgaaaac ccatcacttc atacaaaaat
     2761 ttactcactg gcgagaagcc gacgagaatc agcaggaaac agatcaccac ggagatgatg
     2821 aagctgacga cctggttgcg cgtcatcgcg gaagtcatgc aactgaccgc cagatacgcg
     2881 cccgcgagga aaaagctgcc gatgtaactc gtcaggatga caccgttgtc ggggctgccg
     2941 agataattca ccgtcacggc gatgggaaac gtcagcacca gcgcgatggc caggaacagc
     3001 cacgacgcga ggaattttcc aacaatcgcc tgcgatggag tgatgggcat ggtgagcagc
     3061 agttccattg tgccctggcg ccgttcttcg gaccaaagcc gcatcccggc ggcaggaaca
     3121 agaaacagat agagccacgg atgccatgtg aaaaacgaac ggagcgaagc ctgcccttgc
     3181 tcgaaaaagc cgccgagcat gaatgtgaaa aatccggtca gcagcaggaa aatgacgatg
     3241 aacacaaacg ccaccggcga cgcaaagtaa ccgatcaact cgcgcttggc gatggtcttg
     3301 atgttggccc aggaattcat ttcttttcct ccttcaacgt gtccggcatg gtgatattgc
     3361 ggaacacctc gtccagtcgg ccttcttcag tgtgcaattc ctcgatgcgc cagccgtgcg
     3421 cggcgtcgga aatgctttgc gccagcgcgc cgctcggggt ggtgttgacg ccgccggtct
     3481 tcgggaaaat gcgcgccgtc acgctcgttg cgtcttcttt caagatcatg gttttcttca
     3541 cggtggataa ttgcgaaagt ttctggctca acgcggatgc cgtcacgccg ctgacgcgca
     3601 acgtcactgc gcccgcccat tcggacttct ggcgcaattg ttgcggcgtg
//
LOCUS       DASZMM010000210         6337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3103930, whole genome shotgun sequence.
ACCESSION   DASZMM010000210 DASZMM010000000
VERSION     DASZMM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3103930"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..2803
                     /locus_tag="VG754_04990"
     CDS             278..2803
                     /locus_tag="VG754_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HWF18597.1"
                     /translation="MRKVLYVMVLAGFCGVSAKAAELPAAEHFHANVQPILNQYCSDC
                     HEDGAHKGNVAFDEFKSDDALLTNRTLWFTALKNLRAGLMPPDKKPRPSAEEIKRIEN
                     WIKYDDFGIDPKNPDPGRVTLRRLNRVEYRNTIRDLMGVDYDTTEQFPADDTGYGFDN
                     IGDVLTLSPMLFEKYLDAAKTIISQAVPMVSHTVVERTIPGSAFSKDGGWDSLRLSYY
                     EKGSASKTFQAKNAGHYQLVVDLTATEHFVEDEFDYNKCRLIFKVDGQEVLRKEFTRE
                     GDKPFRFDFTRDWKAGEHQLAFEVEPLTPNEKQPRSLALRIDSVTVRGPMEEKYQVTP
                     KNYARFFPRAVPGSATERRAYARELLTAFAGKAFRRPVDDATLDRLTHLAEDFYNQRG
                     KTFEAGVAHAMTAVLASPQFLFREENIESDHPPGIQPFVDEYALASRLSYFLWSSMPD
                     DELIRLAAAKQLRQNLSAQVKRMLADPRSDGLIKNFTGQWLQARDIETVAIDSRAVLE
                     REDEKDPEVARLRARLRELRKRPREELSQEEHREAHEIYLKLTVNEPRTRLSYELRSS
                     MRREVQMYLGHIVHDDRSVMELIDSDYTFLNERLARHYGLTNLNVSGSDLVRVTLPAN
                     SPRGGVLTMGSVLAVTSNPTRTSPVKRGLFILDNILGTPAPPPPPNVPPLEDSDHEVA
                     GHQPTLREALAMHREKPMCNSCHSRMDPLGLALENFNAMGMWRERERDQAIDPAGKLI
                     TGESFKNVRELKHILATNHRLDFYRTLTQKMLTYALGRGLEYYDVETVDQIVERLDRE
                     DGHFSALLYGIIESSPFQKRREYDTKPVVAPSSGRVEERAERN"
     gene            2806..4209
                     /locus_tag="VG754_04995"
     CDS             2806..4209
                     /locus_tag="VG754_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HWF18598.1"
                     /translation="MKTKHTPTELAAAKQAARIEFSRRHFLRGLGACLALPTFASLGP
                     LKALAASAPKLAATSTGAPIRTAFVFFPNGAIPSSWWPEKDGADFAFSETLKPLEQCR
                     KLMQVMGGLDHRNAIGGPDGAGDHARGTGVFLTGVRLKKSATDIHSGVSIDQIMAREI
                     GHLTRFPSLELTCDTVRKSGACDSDYSCAYQYNLSWNSPTTPMTPETNPRLVFERLFG
                     AGAPNERAEYRKRRQAEQRSILDFVLDDAHRMEKRLDPRDKDKLDQYFTGVREIETRI
                     QKAEKLGPVKDPGNDAPSGVPGDYAEYIQIMFDLMALSFQTDSTRVATLCLAHDGDNR
                     SFSEIGIAEGHHDLSHHQGKAERIKKVAEIDRWYVMQFARFIQKLDAMKDADGKSVLH
                     NSMIVYGGGNADGNAHTHTNLPIVLAGSGGGTLKTGRYVNYKSQPTCNLFMSMADRMG
                     LNSLERFGDSTGRLSDV"
     gene            complement(4251..4691)
                     /locus_tag="VG754_05000"
     CDS             complement(4251..4691)
                     /locus_tag="VG754_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18599.1"
                     /translation="MSAIKQNWPLLMLFAIALLGGCGKKPTLEYVVPNGYTGILKLCG
                     GVADGVTLTPTNGLIVLTFPSSETLNVKGPLPVFEWHKERARYADGTDIPIYGFDSNV
                     SNNIISLRGLGVKNDNRECWYLVGKEGQMPAAMNQFYGFDVPKR"
     gene            complement(4701..6032)
                     /locus_tag="VG754_05005"
     CDS             complement(4701..6032)
                     /locus_tag="VG754_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF18600.1"
                     /translation="MKKLSRRKFLGKSALVAGLAPIAFSGFPFIRSARANEPGASDKI
                     RLGVIGCGDQGLYDFKDFLANPEVDCAVVCDVDDARIARILDTLEKTRGKKADSTKDF
                     RHVLDRKDVDIVLVATPDHWHAMPTVLACQAGKDVYVEKPLAKTIDEGRAMREAARRH
                     DRVVQMGSQWKSCKHIIEATDLIRAGKIGKVHMVRGWAYLDWVPSIGSPADCPPPPGV
                     DYETWLGPAPERPFNPNRFHFKFRWFWDYAGGLMTDWGVHLINMMLMGMGPDSPLNVF
                     STGAKYRFTDNSETPDSQIAVYQFPDYILVWEHKAGLNNGLNGRSWGVEWNGADATII
                     LNDEGYQIIPEPKGGGNIDAAKVPGSGNPHPAHVRNFLDCVKSRQQPVLNLEIGHHVS
                     TVAHLGNIAYRTGRKINWDGKAQKIIGDREADKLVGVKYRKPWHLPYAERA"
     gene            6070..>6337
                     /locus_tag="VG754_05010"
     CDS             6070..>6337
                     /locus_tag="VG754_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18601.1"
                     /translation="MSDSGILSSGIICVETKATPATHLIIVERGYTRSASRDTFSIYR
                     NHSIQPRMKHGLNTEKCRQKPFFKAGKVISFGECFLNRPKWLFTA"
BASE COUNT         1429 a         1815 c         1731 g         1362 t
ORIGIN      
        1 ccaaagccgc caatcgaatg agatggattc ggtgaaccgc gcgacgtccg cgccgggact
       61 tccggcgaac cgtccgctgc gggagacagg agaagttttt ttcatggtca atccgcgcca
      121 ttttgcgagg atatggcggc aaagtcacgc gggaaaggaa accctttgtc atgataaaac
      181 actacatttg tcaggggtca aaactgaata tttcccgtga attcccggtc agaataggtg
      241 atactgacaa tgaaccagaa tcagaacgac ccaattgatg cgcaaagtct tatatgtgat
      301 ggtattggcg ggtttttgcg gcgtttccgc caaggctgcg gaattgccgg ccgccgaaca
      361 ttttcacgcg aacgtccagc ccattctcaa tcaatattgc tccgattgcc acgaagacgg
      421 cgcgcacaag ggaaacgtcg cattcgatga attcaaatcc gatgacgccc ttctcaccaa
      481 tcgcaccctc tggttcaccg cgctcaaaaa tttgcgcgcc ggactcatgc cgcccgacaa
      541 aaaaccgcgc ccttccgccg aagaaatcaa gcgtatcgag aactggatca aatacgacga
      601 ttttggcatt gacccgaaaa atccggatcc cggccgcgtc acgttgcgcc gcctgaaccg
      661 agtcgagtat cgcaacacca tccgcgattt gatgggcgtc gattacgaca cgaccgagca
      721 atttcccgcc gacgacaccg gttacggctt cgacaatatc ggcgatgtcc tgacgctctc
      781 gccgatgttg ttcgaaaaat atttggacgc cgcgaaaaca atcatttccc aggcggttcc
      841 gatggtttcg cacacggtgg tggaacggac gattccgggc agcgcgttca gcaaagatgg
      901 cggttgggat tcgttgcgat tgtcgtatta tgaaaagggt tcagcttcca aaactttcca
      961 ggcgaagaat gcgggccatt atcaattggt ggtggacttg accgcgactg aacatttcgt
     1021 cgaggatgaa tttgattaca acaaatgccg gctcatcttc aaagtggacg ggcaggaagt
     1081 gcttcgcaaa gaattcaccc gcgaagggga caagccgttt cgttttgatt tcacccgcga
     1141 ctggaaggcg ggcgaacatc aactcgcctt tgaagtggaa ccgttgacgc cgaacgaaaa
     1201 gcagccgcgg tcgcttgcgt tgcgaatcga ttcggtgacg gtgcgcggcc cgatggaaga
     1261 aaaataccag gtcacgccga agaattacgc gcgctttttc ccgcgtgcag ttccgggcag
     1321 cgccactgag cgccgggctt acgcgcgtga attgctgact gcttttgccg gcaaggcttt
     1381 ccggcgcccg gtcgatgatg ccactctgga ccgcctcact catttggcgg aggattttta
     1441 caaccagcgc ggcaaaactt tcgaagcggg cgtcgctcat gcgatgacag cggtgctggc
     1501 gtcaccacaa tttttatttc gcgaggagaa catcgagtcc gaccatccgc ccgggatcca
     1561 accattcgtt gacgaatacg cgctcgcatc gcgcctttcg tattttctct ggtcctccat
     1621 gcccgatgac gaattgatcc ggcttgccgc cgcaaaacaa cttcgccaaa acctttccgc
     1681 gcaggtgaaa cggatgctgg cagacccgcg ttcagacggg ttgataaaaa atttcaccgg
     1741 tcaatggctt caggcccgcg acatcgaaac cgtggcgatt gactctcgcg cagttttgga
     1801 gcgcgaagac gaaaaggacc cggaagtggc gcgtttgcgg gcacgattgc gcgagttgag
     1861 gaaacgtccg agggaggaac tcagccagga ggagcatcga gaggcgcacg agatatatct
     1921 gaaacttact gtgaacgagc cgcgcacccg tctgagttac gaattgcgct catccatgcg
     1981 gcgcgaggtg caaatgtatc tcggccacat cgtgcacgac gatcgcagcg tgatggagtt
     2041 gattgacagc gattatacat tcttgaacga acggctcgcg cggcattatg gcctgaccaa
     2101 tttgaacgtc agcggcagcg accttgtccg cgtcacgttg ccggcgaata gtccgcgcgg
     2161 cggcgtgctc accatgggca gcgtgttggc ggtgacttcc aatcccacgc gcacttcgcc
     2221 tgtgaaacga ggcttgttca ttttggacaa tattctcggc acacccgcgc cgccgccgcc
     2281 gcccaatgtc ccgccgctcg aagattccga tcacgaagtc gcgggccatc agccgacgtt
     2341 gcgagaagcc ctggcgatgc accgcgaaaa accgatgtgc aattcctgcc acagccgcat
     2401 ggacccgctc gggttggcgc tggaaaattt taacgccatg ggcatgtggc gcgaacgcga
     2461 gcgcgatcag gcgattgatc ccgcgggcaa gcttatcacc ggcgaatcgt tcaagaacgt
     2521 ccgcgaactc aagcacattc ttgcgaccaa tcatcggctc gatttttacc gcaccttgac
     2581 ccaaaaaatg ctgacgtatg cgcttgggcg cggtttggaa tattacgatg tcgagacggt
     2641 ggaccaaatc gtcgaacgcc ttgatcgcga ggacggacat ttctccgcgt tgctctacgg
     2701 catcattgaa tcttccccct tccaaaaacg ccgggaatat gatacaaagc ccgttgtcgc
     2761 cccatcgtcc gggcgcgtgg aggagcgggc cgaaaggaat tagccatgaa aacgaagcat
     2821 acgccaacgg aactcgccgc cgcaaaacag gcggcgcgca ttgaatttag ccggcgacat
     2881 ttcttgcgcg gcttgggcgc atgtctcgcg ctgccaacct tcgcgtcatt gggcccgctc
     2941 aaggcgctcg ccgcatccgc tccgaaactg gccgcaactt ccaccggcgc gccgattcgc
     3001 acagcatttg ttttctttcc gaacggcgcg attccctctt cttggtggcc ggagaaggat
     3061 ggcgctgatt tcgccttcag cgagacgctc aagccgttgg agcaatgccg caagctcatg
     3121 caggtcatgg gcggattgga ccatcgcaat gccatcggcg ggccggacgg cgcgggtgat
     3181 catgcgcgcg gcacgggcgt tttcctcacc ggcgttcgct tgaaaaaaag cgccaccgac
     3241 attcattccg gtgtttccat cgatcaaatc atggcccgcg aaatcggcca tctcacccgc
     3301 ttcccttcgc tcgaattgac ctgcgacacc gtccgcaaat ccggcgcgtg cgattccgat
     3361 tattcctgcg cgtatcaata caatctatcg tggaattcgc cgaccacgcc gatgacgccg
     3421 gaaacgaatc cgcgcctggt cttcgagcgg ctcttcggcg cgggcgcgcc caatgaacgc
     3481 gctgaatatc gcaaacgccg ccaggccgag cagcggtcca tcctcgattt cgttctcgac
     3541 gacgcgcacc ggatggaaaa gcgtctcgac ccgcgcgaca aggacaagct cgaccaatat
     3601 ttcaccggcg tgcgtgaaat cgagacgcgc atccaaaaag ccgaaaagct tggcccggtg
     3661 aaagaccccg gcaacgacgc gccctccggt gttccgggcg attatgccga atacatccag
     3721 atcatgttcg acttgatggc gctgtcattc cagaccgatt ccacgcgcgt ggcgacgctt
     3781 tgccttgccc atgacggcga caaccgctcc ttcagcgaaa tcggcatcgc tgagggccac
     3841 cacgatcttt cgcaccatca aggcaaggcc gagcgcatca aaaaagtcgc cgagattgac
     3901 cgctggtatg tgatgcaatt cgcgcggttc atccagaagc tggacgcgat gaaagacgcg
     3961 gacggaaaat cggtgttgca caattcgatg atagtctatg gcggcggcaa cgccgatggc
     4021 aacgcccaca cgcacaccaa tctgcccatc gtgcttgcag gctcaggcgg cggcacgctc
     4081 aagaccggcc gttacgtgaa ttataagtcg caaccgacgt gcaatctttt tatgagcatg
     4141 gccgatcgca tgggcctgaa ttccttggaa cgcttcggcg attcgaccgg gcgtttgagt
     4201 gatgtttgag ttttgaatgc cgaagttcag ttcgcaaaac acggagcacc ctaacgttta
     4261 ggaacatcaa agccgtaaaa ttggttcatt gccgccggca tttgcccttc ttttccaaca
     4321 agataccagc attcacgatt gtcgttttta acgccaagtc ccctcaaact gattatgttg
     4381 ttggacacat tgctatcgaa cccataaatg ggaatgtccg tcccgtcggc gtacctcgcg
     4441 cgctctttgt gccattcgaa aacaggtaga gggcctttga catttaaggt ttcagatgaa
     4501 ggaaacgtca gaacgatcaa tccgtttgtt ggcgttaatg tcacgccatc tgccacgccg
     4561 ccgcacagct ttagtatccc tgtgtaacca ttcggtacaa catattccaa agtcggtttt
     4621 ttgccgcatc cgccaagcaa tgcgatcgcg aataacataa gcagaggcca gttctgtttg
     4681 atagcgctca ttgacctgca ttacgccctt tccgcgtaag gcaaatgcca cggtttgcgg
     4741 tatttaactc ctaccaactt gtccgcttcg cggtcgccga taattttttg cgctttgccg
     4801 tcccaattga tttttcgacc agtgcgatac gcgatgttgc ccaaatgcgc gacggtcgag
     4861 acgtggtggc cgatctcaag attgagcacc ggttgctggc gtgatttgac gcaatcgagg
     4921 aaatttcgca cgtgcgccgg atgcggattg ccgctgccgg gcaccttggc cgcgtcaatg
     4981 ttcccgccgc ctttcggttc aggaatgatt tggtagcctt cgtcattgag aatgatggtg
     5041 gcatcggcgc cgttccattc cacgccccag gaacgaccgt tcaggccgtt gttcaagccg
     5101 gccttgtgtt cccaaacgag gatgtaatcg ggaaattgat agactgcgat ctgcgaatcc
     5161 ggcgtttcgg aattgtcggt gaaccggtat ttcgcgcctg tggaaaaaac attcaacggc
     5221 gaatccggcc ccatgcccat cagcatcatg ttgatgagat gcacgcccca atcggtcatc
     5281 aatccgcccg cgtaatccca gaaccagcgg aatttaaaat ggaaacggtt cgggttgaat
     5341 ggccgttccg gcgcgggccc aagccacgtt tcataatcca cgccgggcgg cggcggacaa
     5401 tcagcgggac tgccaatgct cgggacccag tcgagatatg cccagccgcg caccatgtgg
     5461 accttgccga ttttgcccgc gcggatcaaa tcggtggcct cgatgatgtg cttgcaactt
     5521 ttccattgcg aacccatttg cacgacgcgg tcatggcggc gcgcggcttc gcgcatcgcg
     5581 cggccttcgt caatcgtctt ggccagcggt ttctcaacat aaacatcctt gccagcctgg
     5641 caggcgagca cggttggcat cgcgtgccaa tggtccggcg tggcgacgag cacaatgtcc
     5701 acgtctttgc gatcaagcac atggcgaaaa tctttcgtgg aatcggcctt cttgccgcgc
     5761 gttttttcca gcgtgtccag gatgcgggcg atgcgcgcgt cgtccacatc gcaaaccacg
     5821 gcgcaatcca cttccgggtt cgcgagaaaa tccttgaaat cgtaaagtcc ctggtcgccg
     5881 caaccgatga cgccgaggcg tatcttgtca cttgcgcccg gctcattcgc gcgcgctgaa
     5941 cggatgaatg gaaatccact gaaagcgatt ggcgcaagcc cggcgacaag ggcggatttg
     6001 ccgagaaatt tgcggcggga caattttttc ataatgcttt attgacggtg cgaagccaaa
     6061 aaaattccat tgagtgactc gggaattttg tcatcgggaa ttatttgtgt cgagacgaaa
     6121 gccacccctg caacacatct cataatagtg gagcgcgggt atacgcgaag cgccagccgc
     6181 gatacgttct ctatttaccg taaccattca attcagccac ggatgaaaca cggattgaac
     6241 acggagaaat gcaggcagaa gccatttttc aaggcaggaa aggtgatcag ctttggtgag
     6301 tgctttttga accgcccaaa atggctgttc accgctc
//
LOCUS       DASZMM010000211         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2557137, whole genome shotgun sequence.
ACCESSION   DASZMM010000211 DASZMM010000000
VERSION     DASZMM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557137"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..933)
                     /locus_tag="VG754_05015"
     CDS             complement(121..933)
                     /locus_tag="VG754_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF18602.1"
                     /translation="MLHTKQAIVEVSGLSIQREGTRILDDVCWRVEQGQHWAILGANG
                     SGKTSLLSALTGYFMPTAGEIVVLGRRFGRVDWRDLRQHIGLVSSSIRQMMADTEPAL
                     ETVVSGKFAMIDFWGRISRADRERAMQLLRQIECVHLAERPWMVLSQGERQRVLIGRA
                     LMASPRLLILDEPCAGLDPAAREHFLQFLQRLGNRRDAPTLVLVTHHVEEIMPVFSHT
                     LILESGRVLASGKKSTVLSARNLSRAFHAPIALKKKSGRHTLTVKRSSRRII"
     gene            1031..1903
                     /locus_tag="VG754_05020"
     CDS             1031..1903
                     /locus_tag="VG754_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HWF18603.1"
                     /translation="MTKSFQPMQNLARITVLFMALSGFNRALLADVQQSSSPKAGEHP
                     ANLLMHLKPMEHRKPPAQKIAPAKTTRTAYPAPPTQGADGEPRGILAGPPPSDAIVLF
                     DGKDLYEWLGHDNTTPKWTVTNGVLTVLPKSGYLHTKRTFGDCQLHVEWATPQVVVGD
                     SQGRGNSGIFLQGRYEIQVLDSFQNKTYYDGQAGSVYRQYPPLVNACRGPGEWQTYDI
                     IYHAPRFDADGKLTQPASVTVLQNGVLVQDHVAIKGNTMSDHPTYTAHGKAPIELQDH
                     GNPVHYRNIWIREL"
     gene            1936..2718
                     /locus_tag="VG754_05025"
     CDS             1936..2718
                     /locus_tag="VG754_05025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWF18604.1"
                     /translation="MKALVATTLITLGCVLSARAQLHTENVEYKDGDTVLEGYLAYDD
                     SVAHGKRPGVLVVHQWKGLTDYEKKRAEMLAKLGYVAFAADIYGKGVRPKDPKHAGAE
                     AGKYKSNRALLRERVNAGLDVLKKNELTDTSRVAAIGYCFGGTTVIELARSGADIAGV
                     VSFHGALDSPNPEDGKNIKCKVLALHGADDPFVPAADIAAIENEMREAKVDWQLVKFG
                     GAVHSFTIWNADEDHLNGSAYNEKADKRSWEYMKQFFAEIFK"
     gene            complement(2741..>3485)
                     /locus_tag="VG754_05030"
     CDS             complement(2741..>3485)
                     /locus_tag="VG754_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWF18605.1"
                     /translation="EKQEPVLRLPEWPQHELLAAEKELLGFYVTGHPLTPFAPVLQKY
                     ALATTNALAQLPNRTLTRIGGLIAAVQQGVSKKSNKPYAMVTLEDLEGSVQILCMNEN
                     YDKYRPLLVLNKAILVVGEVNNGEDKPKIFPQEIMPLEDAPKKYTRQVHFRLHTAHLK
                     PENIEGIHELASAHPGKCPLFLCFQMPGGESVFVETHEKFSVAPSLNLQQAANERFGD
                     DTYYAKVDSTLPERAPRRWEKRNGDNGDE"
BASE COUNT          802 a          974 c          965 g          744 t
ORIGIN      
        1 aagcggaacg actgggagcg gacgcgttct tccgcaagcc ggagcggttt catgggttgg
       61 tcgccatcgc caaggaaatt gaaaagatgg tcttgttcgc agaagaacgc gacgcgctgc
      121 ttaaattatg cggcgtgagg accgcttgac ggtcaaagtg tggcggcccg attttttctt
      181 cagcgcgatg ggtgcgtgaa aggcgcggga aagattgcgc gcgctcagga ccgtcgattt
      241 tttgccggaa gccaatacgc gcccggattc caaaatcaac gtgtgcgaaa acaccggcat
      301 gatctcctcc acatgatgcg tgaccagcac cagtgtcggc gcatcgcgcc ggttgccgag
      361 gcgttggagg aattgaagaa agtgttcacg cgcggcggga tcaaggccag cgcacggttc
      421 gtccaaaatc agcaaacgcg gagaggccat caacgcgcgg ccgatgagca cacgctggcg
      481 ttcgccttgc gataaaacca tccatggccg ttccgcgaga tgaacacatt cgatttgccg
      541 caacaattgc atggcgcgct cgcggtcggc gcggctgatg cgcccccaga aatcaatcat
      601 cgcgaatttg ccgctgacca cggtttccaa tgccggttcc gtgtcggcca tcatctggcg
      661 gatggatgaa ctgaccaaac cgatgtgctg ccgcaaatca cgccagtcca cgcggccaaa
      721 acgcctcccg agcacgacga tttccccggc ggtcggcatg aaataaccgg tcagcgcgct
      781 cagcaacgaa gttttgccgg aaccgttcgc accgagaatc gcccaatgtt ggccttgctc
      841 gacgcgccag caaacatcat ccagaatacg cgtgccttcg cgttggattg aaaggccgga
      901 cacttccacg atggcttgct tggtgtgcaa catgttttac aagcaagcca tgaatcagca
      961 atgcgggaaa gaaaagtttt ttgacgttgc gattcgacac gatttgtggc tgtctttgcc
     1021 gaatctgatt atgaccaaat catttcaacc gatgcaaaac ttggcgcgga tcaccgttct
     1081 tttcatggcc ttatccggtt tcaaccgtgc gttgctggcg gatgtccaac aaagttcaag
     1141 tccaaaagcc ggcgaacatc ccgcgaacct tttgatgcat ctgaagccaa tggagcatcg
     1201 aaaaccgcct gcacaaaaaa ttgcccctgc caaaacaacc cgaactgcct atccggctcc
     1261 gccaactcag ggagccgatg gcgaaccgcg cggcattctt gccggcccac ctccttcaga
     1321 tgccatcgtt ttgttcgatg gcaaggattt atacgaatgg ctcggtcatg acaacacgac
     1381 gccgaagtgg acggtcacca acggcgttct taccgtcctg ccgaagtccg gttatctcca
     1441 taccaaacgc acgtttggcg attgccagtt gcatgtcgaa tgggccacgc ctcaagtcgt
     1501 tgtcggcgac agccaggggc gagggaacag cggcattttt ctgcaaggcc ggtacgaaat
     1561 ccaggtgctc gattctttcc agaacaagac ttattacgac ggacaggcag gcagtgttta
     1621 tcggcaatat cctccgttgg tcaacgcctg ccgaggtccg ggtgaatggc agacttacga
     1681 catcatttac catgcgccgc gttttgatgc ggatggcaag ctcacccaac ccgcgagcgt
     1741 caccgtcttg caaaacggcg tgctcgtcca ggaccacgtt gccatcaagg gaaacacgat
     1801 gagcgatcat ccaacttaca ccgcgcatgg aaaggcgccg attgaactac aagaccacgg
     1861 caacccggtg cattatcgca atatttggat tcgcgagctt tgaaatataa ctcagaacac
     1921 gctatgttgc ccgccatgaa agcgcttgtt gccacgactt tgatcacatt gggatgcgtc
     1981 ctctccgcgc gggcgcaatt gcacaccgaa aacgtcgaat acaaagacgg cgacactgtc
     2041 ctcgaaggtt atctggccta cgacgattcc gtggcccacg gcaaacgtcc cggcgtgctc
     2101 gtcgtgcatc agtggaaagg actcaccgat tacgaaaaga aacgcgccga gatgctggcg
     2161 aaacttggat atgtcgcgtt cgcggcggac atttacggca aaggcgtccg tcccaaagac
     2221 ccgaaacatg ccggcgcgga agcgggaaaa tacaagtcaa accgcgcgtt gctgcgcgag
     2281 cgcgtgaatg ccggtttgga cgtgctcaag aaaaacgaac ttaccgacac ttcgcgcgtg
     2341 gcggccatcg gttattgttt tggaggcaca actgtcattg aactggcgcg cagcggcgcg
     2401 gacatcgcgg gcgtggtgag ctttcatggc gcgctggatt cgcccaatcc ggaagacggc
     2461 aagaacatca aatgcaaggt acttgcattg catggcgcag acgatccttt tgttccagcg
     2521 gcggacatcg ccgcgattga gaatgagatg cgcgaggcga aggtggactg gcagctcgtg
     2581 aaattcggcg gcgcagtcca cagcttcacc atttggaacg ccgacgaaga ccatttgaac
     2641 ggttccgctt acaatgaaaa agccgacaaa cgctcatggg aatacatgaa gcagtttttt
     2701 gcggaaattt ttaagtgacc agcacgtttt atctggagcg tcactcgtcc ccattgtcgc
     2761 cgtttcgttt ctcccaacgg cgtggagcgc gttcaggcaa ggtgctgtcc accttggcgt
     2821 aataagtgtc atcgccaaac cgttcgttcg ccgcttgttg caggttgagc gacggcgcga
     2881 cggaaaattt ctcgtgcgtt tcgacaaaca cactctcgcc acccggcatc tgaaaacaaa
     2941 ggaacagcgg gcatttgccg ggatgggctg acgccagttc gtggatgcct tcgatgttct
     3001 ccggcttcaa atgcgccgtg tgcagccgga aatgcacctg cctggtgtat ttttttggcg
     3061 cgtcttccaa cggcatgatt tcctgcggaa aaatcttcgg cttgtcctcg ccattattga
     3121 cttcgccgac gaccaggatg gccttattca agaccagcaa gggccggtat ttgtcgtaat
     3181 tctcgttcat gcaaaggatt tgcacggagc cttccaaatc ttcaagtgtc accatcgcat
     3241 aaggtttgtt gctcttcttg gaaacacctt gttgcaccgc cgcgatgagc ccgccgatgc
     3301 gcgtgagcgt gcggttgggc aattgcgcaa gcgcattggt cgtggccagt gcgtattttt
     3361 gcaacaccgg cgcgaacggc gtcaatggat ggccggtgac atagaacccg agcaattcct
     3421 tttccgccgc gagcaattcg tgctggggcc attcgggcaa acgcaaaacc ggttcctgtt
     3481 tctcc
//
LOCUS       DASZMM010000212         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2557229, whole genome shotgun sequence.
ACCESSION   DASZMM010000212 DASZMM010000000
VERSION     DASZMM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557229"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VG754_05035"
     CDS             complement(<1..264)
                     /locus_tag="VG754_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18606.1"
                     /translation="MKLASGVHSVHSLAERETFHPVIGPVAEAEALYVKQLRLPDRLA
                     NHTGEFVIWDVGLGAAANVLTVLRATRESHCPLRILSFDHTLEP"
     gene            386..691
                     /locus_tag="VG754_05040"
     CDS             386..691
                     /locus_tag="VG754_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HWF18607.1"
                     /translation="MYVQTDTQQLLAEYVGNGSETAFRELVARYLNFVYSTALRVVGG
                     NAHLAEDVAQMVFINLARKFTAMTAAISQIGKRSTSSCRRHNDRATQSCFIALSNAA"
     gene            complement(655..1743)
                     /locus_tag="VG754_05045"
     CDS             complement(655..1743)
                     /locus_tag="VG754_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWF18608.1"
                     /translation="MAHVKLHIPGPVEVSEKTFRAFASPMIGHRGQGFKDLYAKIQPQ
                     LQSLLYTKQLVYLSTSSAWGVMEGAIRNLVAKKVLNCMCGAFSDKWLDVSQRCGKQAE
                     GLKVEWGSPIRAEQIDAKLATGQFDALTLIHNETSTGVMSPLAEIAALKKKYPDVMFI
                     VDSVSSLSATKIEFDALGIDVLLAGTQKAFAMPPGLAVFACSPAALAKAATMKDRGYY
                     FDFVEFQKNAEGNMTPSTPSIGHVYALASKLDDFFTEGLEARYARHAKLSQLTRDWAA
                     RHGFTLFPEKGFESVTLTCVNNGAKPGGRAVDVAKLQKLVKDQGFLIDGGYGKIKGTT
                     FRISNMGDETEATMNQLYAALDNAMKQL"
     gene            1850..2473
                     /locus_tag="VG754_05050"
     CDS             1850..2473
                     /locus_tag="VG754_05050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18609.1"
                     /translation="MKFPESKLAHKYLDGLKGLEIGGSAHNSFGLNTKNADVNPGLGT
                     VYKKAELKECGEALPVDIVAPGDELPLPDESYDFVISSHVIEHFFDPIRAIKEWWRVI
                     KPGGYIFIIAPHKERMFDKDRPRTKLAELIDRHEGRIQRTGKADQHFSVWIPEDLLEL
                     CRYLNMEVVEFQDPDDKVGNGFTVVIQKPGGNQLWTFFVRAFRKAVR"
BASE COUNT          617 a          735 c          726 g          629 t
ORIGIN      
        1 cggttccaac gtatggtcga agctgagaat gcgaaggggg cagtgacttt cgcgggtggc
       61 gcgaagaacc gtcagcacat tcgcggctgc gccgaggccc acgtcccaga tgacaaattc
      121 gccggtgtga ttggcaagcc ggtcgggaag gcgcaattgt ttgacgtaga gcgcttcggc
      181 ttccgcgacc gggccgatga ccggatgaaa cgtctcgcgt tccgcaagcg aatggacgct
      241 gtgcacgccg ctggctagtt tcacgatttt atacccggtt gtttcttgca cacttcatgt
      301 taaatggcca caaagaggca cacaaggcgc ataaaccggc tctgaaaatt tttgttcggt
      361 tttgccgacc tgcggggctt tactgatgta cgtgcaaaca gatacgcaac aattgctcgc
      421 ggaatatgtc gggaacggct cggaaacggc ctttcgcgag ctggtcgcgc gttatctcaa
      481 ttttgtgtat tcaacggctt tgcgggtggt cggcggaaat gcgcatttgg ctgaggacgt
      541 ggcgcagatg gtcttcatta atctggcacg gaaattcacc gcaatgacag cggcgatttc
      601 acagattggg aagcgcagca cctcttcgtg ccgccgtcac aatgaccgcg caactcaaag
      661 ctgcttcatc gcattgtcca acgcggcgta aagctggttc atcgtcgctt cggtttcgtc
      721 gcccatgttg gaaatgcgga aggtcgtccc tttgattttt ccatagccgc cgtcaatgag
      781 aaacccttgg tccttcacca gtttttgcag cttggcaacg tcaaccgcgc gtccgcccgg
      841 ttttgcgccg ttgttgacgc aggtgagcgt gaccgattca aaaccttttt ctgggaacaa
      901 cgtgaaacca tgccgcgcgg cccaatcgcg ggtcaactgc gacagcttcg cgtgacgcgc
      961 ataacgcgct tcgagccctt cggtgaagaa atcgtccagc ttcgaagcca gcgcataaac
     1021 atggccaatg ctcggcgtgc tgggcgtcat gttgccctcg gcgttttttt ggaattcgac
     1081 gaaatcgaag taatacccgc ggtccttcat cgtcgcggcc ttggcaagcg ccgcgggcga
     1141 acaggcgaat actgccaagc ccggcggcat ggcaaaagct ttttgcgttc ccgccagcaa
     1201 cacatcaatg cccaacgcgt cgaactcaat cttcgtcgcg ctcaacgaac tcaccgagtc
     1261 cacgatgaac atcacgtccg gatatttttt cttaagcgcg gcaatttctg ccagcgggct
     1321 catcacgcca gtcgaagttt cattgtggat gagcgtgagg gcatcgaact ggccagtggc
     1381 gagcttcgcg tcaatctgtt cggcgcggat gggcgaaccc cattcgacct tcagcccttc
     1441 ggcctgcttg ccgcaacgtt gcgaaacatc cagccacttg tccgagaacg cgccgcacat
     1501 gcaattgagc acttttttgg caaccagatt gcggatggcg ccttccatca cgccccacgc
     1561 ggacgacgtg ctgagataaa cgagctgttt ggtgtaaagc agggattgca actgcggctg
     1621 gatctttgcg taaaggtctt tgaaaccctg gccgcgatgg ccaatcatcg gcgaagcaaa
     1681 cgcgcgaaaa gttttctcgc tgacttcgac cggtcccggg atgtgtagtt tgacatgtgc
     1741 cataaatagt ttgtgaaatc tttcacaagc gcgacgcttt ggcaaggttg aaaagaacaa
     1801 cgaaccagtt tagaatcgtc gtcattcaaa aggcttcatc caacacacaa tgaaatttcc
     1861 tgaatccaaa ctggcccata aatatctgga cggccttaaa ggactggaga tcggaggcag
     1921 cgcgcacaac agtttcggtt tgaacaccaa gaatgcggac gtgaatcccg gcttgggcac
     1981 ggtttacaaa aaagctgagc tgaaggaatg cggcgaagcg ttgccggtgg acatcgtcgc
     2041 gccgggagat gaacttcctt tgccggatga atcatacgat ttcgtcatct catcgcatgt
     2101 catcgagcat tttttcgatc cgattcgcgc gatcaaggaa tggtggcgcg tcatcaagcc
     2161 cggcggatac atcttcatca tcgctccaca taaggaacgg atgttcgaca aggaccgccc
     2221 ccgcaccaaa ctggccgaat tgatcgaccg ccacgaaggc cgcatccaac gcaccggcaa
     2281 agccgaccag catttttctg tttggatacc ggaagatttg ctcgaactat gccgctattt
     2341 gaatatggaa gttgtcgaat tccaggaccc ggatgacaag gtaggaaacg gattcaccgt
     2401 ggtgatccaa aagccgggcg gaaaccagct ctggactttt tttgtccgag cgttccgaaa
     2461 agccgtgcga tgacatattt ctgagttatt ggaatcatca ccccgcgcga attattggcg
     2521 cgtcttgcgc gacaacgcgg ctgattctca ttccagcgcc tcgcggattt ttttggcgtc
     2581 ctcgtcgtcg ggagatttgg agataaaatc gttaatttgc tccttcgctt tcttgtgatc
     2641 atcctcatca gacattaatc cgtacacaat cgcagtatcc aatgtccata cccgccaaag
     2701 ttcttga
//
LOCUS       DASZMM010000213         5736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1465859, whole genome shotgun sequence.
ACCESSION   DASZMM010000213 DASZMM010000000
VERSION     DASZMM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465859"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VG754_05055"
     CDS             <1..306
                     /locus_tag="VG754_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWF18610.1"
                     /translation="QWARAKSFDTFTPLGPYVETKIDPHDLTIQLFQNGQLRQNSNTN
                     QLIFNCYHLVSFISTNMTLLPGDIICTGTPSGIGPIESGDRIEVRIQGLAPLVNTVK"
     gene            385..708
                     /locus_tag="VG754_05060"
     CDS             385..708
                     /locus_tag="VG754_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18611.1"
                     /translation="MNDTTKILCAVGLGIYSSAGIYFYSVLVSRTQNLGLRLFLYLGC
                     VALFIFGGLVGCAALWGDFDHDRQKPTSHESNIAHFVLALWLGIFVIYIVLDGRRILR
                     RIRGY"
     gene            832..2559
                     /locus_tag="VG754_05065"
     CDS             832..2559
                     /locus_tag="VG754_05065"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWF18612.1"
                     /translation="MRWTQTLIPTLKETPAEAEITSHKLLLRAGLVRKLSGGLYTFLP
                     LGLRILKKIEQIIREEMDRSGAIELLLPVLHPKEIWEQSGRAEPWRNTLFKVKDSGNR
                     EWFLSPTAEEAVTVVAGAEINSYRQLPKNLYQIGVKFRDEVRPRFGLMRAKEFIMKDA
                     YSFDSSDELAQVSYLKMYDAYARIFQRCGLKAIAVEADSGAMGGSFSAEFMIPAETGE
                     DEVVFCESCGYAANVEKAISGVPKTAAREIGAAIKKFATPGVVTIEALAKPPYNVPAN
                     RQIKTLVHITDSKPVIILVRGDDQLNETKFAGKIGTTVFRPATAEEIFELMGAHPGSL
                     GAVTSSLKNAGINVLADERLRGANGMVTGANEDGHHFNNVSIERDIQVSQWADLRTVT
                     EGEPCAKCGKPLKIRRSIEVGHVYKLGTKFSVALGAGFLDESGVRKPCVMGCYGIGVT
                     RTLQAIIEQSNDKDGIIWPLNAAPYTVCITPLSVTPGGPVMTLAEKIYEELTARGVDV
                     ILDDRDERPGVKFKDSELVGFPIRLGIGEKSLAKGEVELKLRGGALTPIKSEEAVGKI
                     MEIIGKGAA"
     gene            2735..4303
                     /gene="metG"
                     /locus_tag="VG754_05070"
     CDS             2735..4303
                     /gene="metG"
                     /locus_tag="VG754_05070"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWF18613.1"
                     /translation="MEKKRFYITTAIDYVNGEPHLGHAYEKVIADVIARARRSLGEEV
                     FFLTGLDEHGQKVQQVALAQGKTPQAYCDELSVVWKSLATKLALTNDDFVRTTEPRHK
                     EAVQAILTKLHDGGFFYKGEYRGYYSVREETFLTDKDRLPDGSFPAQYGEVTQLVEEN
                     YYFKLKDQQAWLIDYIEKNPGFIQPDYRRNEVLGFLKNEELEDLCISRPSNRLNWGIP
                     IPFDPNYVTYVWFDALVNYASIPIAHGDPLVCSAIHSALRTPHSAISLWPADIHIIGK
                     DILKFHAVYWPIMLKAMGLPLPKLVLAHGWWQKEGERLSKSTGNIVDPIAVIDEWGLD
                     AFRFYVVRELAIGPDGNWTDAGFKARYNAELANGLGNLVNRSLSMLKRYRNGIVPERS
                     DELSADATKIISETRAQLEQNQLQAALVSIWGLVNRANQYVDQTAPFKLAKDPSQAKR
                     LDEVLYNLAEITRILAVLLWPFLPGTASKIYAQLGLSGEPNQFSAAAWGGLIAGQAIG
                     EPAALFPRRDIVKP"
     gene            4423..>5736
                     /locus_tag="VG754_05075"
     CDS             4423..>5736
                     /locus_tag="VG754_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18614.1"
                     /translation="MSLALLAARFACAQTLSLPPRPADAPSGSAFAQSVAALQPAERE
                     EKILEQITNGNVPDFLRKLSPVHTEILRDGKTNSATYYVTPDYLAVGSDDDYFLTPLS
                     PIAAQKVADALHCSLPTRKMVDEIYSNAIVKLAPSPIPPSPVMGTIPVFIQHNQTIRE
                     QRKLELATHPLGALVTGHKKDVIISNRLNERRGRVAIYGWHKLDGKPIQPLTIVHKDT
                     YADYSHGIRLVQLSLTVDGNPQTIPDVLADSVLSTLLSDEGPILNPKYPPVALSSPYI
                     PSANASQTNEHASLEFIVLPGFQQSAFDEQTISYSMEPEVKVLINAPAKLSSGKKLKL
                     VFFALPNGNTTEQTIGHQLKPRDDWHYDIQHIGAQTRFLRQEFKDCSLVVVYLEAAQK
                     SWPMWRKKHADHPELIPQIIDSITKRFTDFDLRIILSAHSGGGSLI"
BASE COUNT         1372 a         1503 c         1559 g         1302 t
ORIGIN      
        1 caatgggcgc gcgcgaaatc gttcgacact ttcacgccgc tggggccgta tgtggaaaca
       61 aagattgatc cgcatgattt gaccattcaa ctattccaga acgggcaatt gcggcagaac
      121 tcgaacacca atcaactcat cttcaattgc tatcacctgg tcagtttcat ctcgaccaat
      181 atgaccctgt tacccggcga catcatctgc acaggcacgc ccagcggcat cggcccgatc
      241 gaatccggcg accgcattga agtgcgaatc caagggcttg caccgctggt gaacacggtg
      301 aaatagaagc cgacttaaac ctcatctcgt gaaacaggag agccggcaca aacccctgat
      361 cattatctgt taagtgtcat ttcgatgaac gacacaacga agatactttg tgctgtcgga
      421 ttgggaattt actcctctgc cggaatttat ttctattcag ttcttgttag ccgcacacag
      481 aatcttggat tgcgactttt cctttatctg ggctgcgtag ccctatttat tttcggcggg
      541 cttgtgggat gcgctgcgct ttggggtgat tttgaccatg acagacagaa gccaacatca
      601 cacgaaagca atattgcaca ttttgtgcta gcattatggc ttggcatttt cgtcatttac
      661 atcgttctgg atgggcgcag aattctacgg cgtatccgtg gttattgatg gcgcctgaca
      721 gcgcgctgga gccaaccccg atagcattcc ggggctgccc ttccgtcgtc gttcagaaga
      781 tatggcgggc atggctcgct ggtttgaaac tagaattttg aaattgataa tatgcgttgg
      841 acccagacac ttattccgac attgaaggaa acgcccgccg aggcggagat cacgtcgcac
      901 aaattattgc tgcgcgcagg cctcgtgcgc aagctcagcg gtgggctcta tacttttctg
      961 ccgctcggct tgcgcatatt gaaaaaaatc gagcaaatca ttcgcgaaga aatggaccgt
     1021 agcggcgcga ttgaattgct gctccccgtg cttcatccaa aagagatatg ggagcaaagc
     1081 gggcgcgccg agccttggcg gaacacgttg ttcaaagtca aggacagcgg aaatcgcgaa
     1141 tggtttttaa gcccgacagc ggaagaggca gtaacggttg ttgccggcgc ggaaatcaat
     1201 tcctatcgcc agttgccgaa aaatttatat caaatcgggg tgaaattccg cgacgaggtc
     1261 cgcccgcgct ttgggctgat gcgcgcaaag gagttcatca tgaaggacgc gtacagtttt
     1321 gattcgagcg acgaactggc gcaggtgagt tatctgaaaa tgtatgatgc ctatgcgcgc
     1381 atttttcagc gctgcggtct aaaggcgatc gcagttgaag ccgacagcgg cgcgatgggt
     1441 ggaagttttt cagccgaatt tatgattccc gccgaaaccg gcgaggacga agttgtgttt
     1501 tgcgaaagct gcggctacgc ggcgaatgtt gaaaaggcga tcagcggcgt tcccaaaacg
     1561 gcggcgcgtg aaatcggcgc ggcgattaaa aaatttgcca cgcccggcgt ggtgacgatt
     1621 gaagcgcttg cgaaaccgcc ttacaacgtg ccggcaaatc gccaaatcaa aacgctggtc
     1681 catatcacgg acagcaaacc agtcatcatt ttggttcgcg gcgacgatca attgaacgaa
     1741 acgaaattcg ccgggaagat tggcacgact gtttttcgcc cggcgacggc ggaagaaatc
     1801 tttgaattga tgggcgcgca cccgggaagt ttgggcgcgg tgacaagttc gttaaaaaat
     1861 gccgggatca acgttctcgc ggatgaacgt cttcgcggcg caaacggaat ggtgaccggg
     1921 gcaaatgaag acggtcacca tttcaataat gtttccattg agcgcgatat tcaagtttcg
     1981 caatgggcgg acttgcgaac ggtgacggag ggtgagccgt gcgcaaaatg cggcaaaccg
     2041 ctgaaaattc ggcgctccat cgaggttggc cacgtttaca aactcggaac aaaattcagt
     2101 gtcgccctcg gcgcgggatt tttggatgaa agcggtgtgc gcaaaccttg tgtgatgggc
     2161 tgctacggca tcggtgtcac gcggaccttg caggcgatca tcgaacaatc gaacgacaag
     2221 gatggcatca tctggccgtt gaatgccgcg ccgtacaccg tttgcatcac gccgttgagc
     2281 gtcacgcccg gcgggccggt catgacattg gccgaaaaga tttatgagga actgaccgcg
     2341 cgcggagtgg acgtcattct ggatgaccgc gacgaacggc ctggcgtgaa gttcaaggac
     2401 tctgaactgg tcggttttcc catccgtctc ggcatcggcg aaaaatcact cgccaagggc
     2461 gaagtggaat tgaaacttcg cggcggggca ttgacaccta taaaaagcga agaggcggtc
     2521 ggaaaaataa tggagatcat cgggaaaggc gcagcgtaat cctatcgcag aattgcattc
     2581 tgcgtgggcg tcaatttgtc actttactat cttttgccgc ggtgccttgc atttgatcat
     2641 ctgccggctg caagcggcga tacagcagat tgcaaatctc cgctactagc tccgcagttg
     2701 ccaaccggcc ttctgtcagc caaactgatt gcacatggaa aagaagcgat tttacatcac
     2761 aaccgcgatt gattacgtca acggtgagcc gcatctcggc cacgcctacg agaaggtaat
     2821 cgccgacgtg atcgcgcgcg caaggcggag tttgggcgag gaagtttttt tcctgaccgg
     2881 cctcgacgag cacggtcaaa aggttcaaca ggtggcgctg gcgcaaggca aaacgccgca
     2941 agcctattgc gatgaattat ccgtggtttg gaaatcgctt gccacaaagc tcgctctgac
     3001 gaatgacgat tttgtccgca cgaccgagcc gcgtcacaag gaagccgtgc aggcgatcct
     3061 caccaagctt catgacggcg gttttttcta caagggtgaa tatcgcggtt attattcggt
     3121 gcgggaggaa acgtttctca cggacaagga ccgtttgccg gatggctctt ttcccgcgca
     3181 atacggcgag gtgacgcagt tggtcgagga aaattattat ttcaaactca aggaccagca
     3241 ggcgtggctc attgattata tcgaaaagaa tcccggcttc atccagcccg attaccgccg
     3301 caacgaagtg ctcggttttc tcaagaacga ggaattggaa gacctgtgca tcagccgccc
     3361 atcgaaccgt ctgaattggg gcatcccgat tccatttgat ccgaactacg tcacctacgt
     3421 ctggttcgac gcgctggtga attacgccag cattcccatt gcgcacggcg atccgttggt
     3481 gtgttcggcc attcactccg cactccgcac tccgcactcc gcaatttcat tgtggcctgc
     3541 cgacatccat atcatcggca aggacatttt aaaatttcat gctgtctatt ggcccatcat
     3601 gctcaaagcg atggggctgc cgttgccgaa actggtcctc gcgcacggtt ggtggcagaa
     3661 ggaaggcgaa agattgagca agagcacggg aaatattgtg gatcccattg cggtcattga
     3721 cgaatggggt ttggacgctt ttcgttttta tgtcgtgcgc gaacttgcca tcggcccgga
     3781 cggcaactgg actgatgcgg ggttcaaggc gcgttacaac gcggagttgg cgaacggtct
     3841 tggcaatctc gtcaaccgct cgctttcgat gctcaagcgc tatcgaaacg gaatcgtgcc
     3901 ggagcgttcg gacgaattga gcgcggacgc gacaaaaatt atttccgaga ctcgcgcgca
     3961 gttggaacaa aaccaacttc aagccgcgct cgtgagtatc tgggggttgg tcaatcgcgc
     4021 aaatcaatat gtggaccaga ccgcgccgtt caagttggca aaggacccgt cgcaggcgaa
     4081 gcggctcgat gaagtgcttt acaatcttgc tgaaatcaca cgcattctgg ccgtgttgct
     4141 ctggccgttt ttacccggca cagcatcgaa gatttatgct cagctcggac tttccggcga
     4201 gccgaatcaa ttttccgccg cggcgtgggg cggtctcatt gctggccaag ccattggcga
     4261 acccgccgcg ttgtttccgc gccgggacat cgtgaaacca tgaattgccc cgttcggtgt
     4321 ccagccttta ggctgttccg ggccgggaac acgctaaagc gtggacacca aacagcgcgg
     4381 cggcgagggt ggggcatatc actttttaaa tcggttctat ccttgtcact ggcattgttg
     4441 gccgctcgtt tcgcctgcgc gcaaacactt tcgctgcctc cgcgtccagc cgatgcaccg
     4501 tcaggcagcg cgttcgcgca atcggtcgcg gcgctccaac ccgccgaacg cgaggaaaaa
     4561 atcctcgaac agatcaccaa cggcaatgtg ccggattttc tgaggaagct ttcgccggtt
     4621 cacactgaaa tacttcgtga tggtaaaacc aattcggcca cttattatgt cacgcccgat
     4681 tacctggccg tcggctctga cgatgattat tttctgacgc ctctttcacc catagccgcg
     4741 caaaaggtcg cagacgcgtt gcattgcagt ttgccaacgc gaaaaatggt ggatgaaatt
     4801 tattccaatg ccatcgtgaa gcttgcgcct tcgcccattc cgcccagtcc ggtgatggga
     4861 accatcccgg ttttcatcca acataatcaa accattcgcg agcagcgtaa actggaactg
     4921 gcaacgcatc cgctcggcgc gctcgtcact ggacataaga aagatgtgat catttccaat
     4981 cggctgaatg aaagacgggg acgcgttgca atctacggct ggcacaagct ggacggtaaa
     5041 ccaatccagc ctctgactat cgttcacaaa gatacttatg ccgattacag ccacggcatt
     5101 cgtctcgtgc aactttcgct tacagtggat ggcaacccgc aaaccattcc cgatgtgctt
     5161 gcggattcag tcctctcaac gctgttaagc gacgaaggcc cgatactcaa tccgaagtat
     5221 ccgcccgttg ccctatcttc tccgtacatt ccatctgcaa acgcgagcca gacaaacgaa
     5281 catgcgagct tggaatttat tgtattaccc ggtttccagc agagcgcatt cgacgaacaa
     5341 acgatttcct acagcatgga accggaagtg aaggtgctta tcaatgcgcc agcgaaattg
     5401 tcgtccggca agaaactgaa acttgttttc ttcgcgttgc ccaatgggaa tacgacagaa
     5461 caaaccatcg gacatcaatt aaagccgcgt gacgactggc attacgacat ccagcacatc
     5521 ggcgcgcaaa cgcggtttct ccgacaggaa ttcaaggatt gcagtttggt ggtcgtttat
     5581 ctggaggcag cacaaaagag ctggcccatg tggcgaaaga aacatgcgga ccatccggag
     5641 ttgattccgc aaataattga ttccatcacg aagcgattta ccgatttcga tttgcgaatc
     5701 atccttagcg cgcatagcgg gggcggcagt ttgatt
//
LOCUS       DASZMM010000214         3895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_368665, whole genome shotgun sequence.
ACCESSION   DASZMM010000214 DASZMM010000000
VERSION     DASZMM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368665"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VG754_05080"
     CDS             complement(<1..461)
                     /locus_tag="VG754_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWF18615.1"
                     /translation="MPTITISVQRSAAAPDESPVFESHQVSVGEKASVLDALFALQRG
                     PCPDLAFRFSCRVGMCGSCAMVVNGREQLTCSTLVKTIGDTLRVQPLRNLPVTRDLAV
                     DMKPFFAAYQRVMPQFIPRDDLDKGDFYPIPHDSKEGRALNHQPQCIDCGA"
     gene            837..1724
                     /locus_tag="VG754_05085"
     CDS             837..1724
                     /locus_tag="VG754_05085"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HWF18616.1"
                     /translation="MDAPENFYSLVENTCRDLYIKSLKEIPPDVVEAIRQAAQRETKE
                     VGRRIFSHYLQSIELGRKQEMMVCQDTGIPIYWVEIGGNMRLDGARLSEAIVRGTERA
                     TREHPLRSSIVSPLTRENRQTSTGDRIPIIHYDFVRDSDVLEILFMPKGSGSENMSFM
                     KMLVPADGINGIKRFVLEQVVGAGAKPCPPTIVGVGIGGSSDLCMTLAKKATTRPLGS
                     KNPDAKLAALEQELFEAINQTGIGPQGLGGDTTALGVHIESAWTHITCNPVAVNLQCW
                     RAERRRAKIWVDGRVETGF"
     gene            1725..2318
                     /locus_tag="VG754_05090"
     CDS             1725..2318
                     /locus_tag="VG754_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HWF18617.1"
                     /translation="MAEYRFKTPLKEADVRQLRVGDSVLLDGVVFGVRDANMIRVFDQ
                     QKAPPVDWRGAALLHTAPNVKKNASGKYEPISVGTTTSMRMDRFTEGLLRDHGVRAIL
                     GKGGLSSRSSELMAQYGACYLSVTGGAAAMETLQIEEIEAVYWEDLMPECIWQFRFNG
                     LGPLTVGIDAHGGNLQLDVQAQAQQKTAELLRKMGVV"
     gene            2318..2665
                     /locus_tag="VG754_05095"
     CDS             2318..2665
                     /locus_tag="VG754_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18618.1"
                     /translation="MEKRFQNYRGTWAWLAQRFSAIGLFVLIPIKIYSGWAAKDKVPY
                     PDFLGSPKQVHYNAAIDIGLLLFFLIHAFYGLRVILMDVGLIREDSFFWRTMIIALGM
                     FGAVVWFVYIRGE"
     gene            complement(2693..>3895)
                     /gene="dctA"
                     /locus_tag="VG754_05100"
     CDS             complement(2693..>3895)
                     /gene="dctA"
                     /locus_tag="VG754_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HWF18619.1"
                     /translation="FIKLIRMMIAPIIFTNVVVGIAGMDSLKRVGRIGLKSFLYFEVM
                     TTLALAIGWTVAKVFQPGAGMNADISQLNISDLQSTLAAAKAPHGAVEFLLNVIPKSV
                     VGSFADGEILQVLFFSVIFGMAMSGMGEANRPIIRALEQVSKALMRMIAMIIKFAPLG
                     VFGAMSFTIAKFGVGSLVSLAKLMACVYLTCIFFVVICLGSLLRLNGLSIWKMLHYLR
                     DEFFIVFGTASSEAVFPRLLAKLEQLGCSKPLVGIVLPSGYSFNLDGSSVYLTMGALF
                     IAQATNTHLTLGQELAVLFVCLLTSKGAAAVVGSSFITLAATLSSMHTIPVEGMVIIL
                     GVDWFMAQARAMTNMIGNGVATVVIARWENELDREKAVRVLNGELAELAVNINEKAAA
                     SVPVQEQV"
BASE COUNT          935 a         1027 c         1094 g          839 t
ORIGIN      
        1 cacgcgccgc aatcaatgca ttgcggttga tgattcagcg cgcggccttc cttgctgtcg
       61 tgcgggatgg gataaaaatc gcccttgtcc aaatcgtcgc gggggatgaa ttgcggcatc
      121 accctttggt aggcggcgaa aaatggtttc atgtccacgg caagatcgcg cgtgacggga
      181 aggttgcgaa gcggttggac gcgcaacgtg tcgccaatgg ttttgaccag cgtgctgcag
      241 gtcaactgtt cgcggccatt caccaccatc gcgcacgaac cgcacatgcc gacccggcag
      301 gaaaagcgaa acgccaggtc gggacacggc ccgcgctgca acgcaaacag ggcatcgagc
      361 acgcttgcct tctcaccgac ggaaacctgg tgcgattcga agactggact ttcatccggc
      421 gctgcggcgc tgcgttgcac gcttatcgtg atggttggca caaggcaatc gtaatgggcc
      481 gaaaggaagt tgcaattgtt ttgaaaattt ccactcctag ttatgaatcg agttacttcc
      541 agctttacct cctatacgac cctcaccccc caccccccaa aaaaaagcga ataaatgcga
      601 aaaaaggcga gaaaatgcga aaacacgggc gttttcgaca gcaaaacgtt ttccggactg
      661 ggcaagcttc cgaaatgcag gtctcttatc aatagttccg acactcatgg ccgccattct
      721 tgcatatcaa gtaggacact aactgtccaa gtaactcatt ctggttcggt ccgtctggaa
      781 agatttagaa aatgctcgcg gcattgtttt cggccagtta agttccagtc cgacacatgg
      841 acgcgccgga aaatttttac agcctggttg aaaacacctg ccgcgatctt tacatcaaat
      901 cgctcaagga aatcccgccc gatgtggtcg aagccatccg ccaggcggcg caacgggaaa
      961 ccaaggaagt tggccggcgc attttttcgc attacctgca atccatcgaa ctcggccgca
     1021 aacaggagat gatggtttgc caggacaccg gcattccaat ttattgggtg gagattggcg
     1081 gcaatatgcg tctggacggc gcgcgactca gcgaggcgat tgttcgcggg acggaacgcg
     1141 cgacgcgcga gcatccgttg cggtcgagca tcgtttcgcc gctcacgcgc gaaaatcgcc
     1201 agaccagcac tggtgacaga attcccatca ttcactacga tttcgtgcgc gactcggacg
     1261 tgctggaaat tttgttcatg cccaaaggtt ccggctcgga aaacatgtcg ttcatgaaaa
     1321 tgctcgtgcc ggcggacggc atcaacggca tcaaacggtt cgtgctggaa caggttgtcg
     1381 gcgcgggcgc taaaccttgt ccaccgacga ttgtcggtgt cggcattggc gggtcgtcgg
     1441 atttgtgcat gacattggcg aaaaaagcca cgacgcgtcc gctcggaagc aaaaatccgg
     1501 acgcaaaact tgccgcgttg gaacaagaat tgtttgaggc catcaatcaa accggcatcg
     1561 gcccgcaagg tttgggcggc gataccacgg cgcttggtgt gcatatcgag tctgcgtgga
     1621 cgcatatcac ctgcaatccc gtggctgtga atctgcaatg ctggcgggcc gaacgccgcc
     1681 gcgcaaaaat ttgggtggac ggccgcgtgg agactggatt ttaaatggct gaatatcgtt
     1741 tcaaaactcc gttgaaggaa gctgacgtgc gtcagttgcg tgtgggtgat tcggttttgc
     1801 tcgacggcgt cgtgttcggc gtccgcgacg caaatatgat ccgagttttt gaccagcaga
     1861 aagcgccgcc ggtcgattgg cgcggcgcgg cgttgttgca cacagccccg aacgtcaaaa
     1921 aaaacgcctc cggaaaatac gagccgattt ctgtcggcac gacgacgagc atgcgcatgg
     1981 accgtttcac ggaagggttg ctgcgcgacc atggtgtgcg ggcgattctt ggcaagggcg
     2041 gtttgtcatc gcgcagctcg gaactgatgg cgcaatatgg cgcgtgttat ctgagtgtca
     2101 cgggcggcgc ggcggcgatg gaaacgttgc agattgagga gatcgaggcg gtttattggg
     2161 aagacttgat gcccgaatgc atctggcaat ttcgtttcaa cggacttggg ccgttgaccg
     2221 tcggcattga cgcgcacggc ggaaaccttc aactcgacgt gcaggcgcaa gcgcaacaaa
     2281 aaactgcgga attattgcgc aagatgggag tcgtctgatg gaaaaacgtt ttcaaaatta
     2341 tcgcgggaca tgggcctggc tggcgcaacg gttcagcgcc atcggcctgt tcgtgttgat
     2401 cccgatcaag atttactcag gctgggcggc gaaggacaaa gttccctatc ctgattttct
     2461 cggttcgcca aaacaggtcc attacaatgc cgcgatcgac atcgggttgc tgctgttttt
     2521 tctcattcac gcgttctatg gattgcgggt gattctcatg gatgttgggt tgattcgtga
     2581 agactcattt ttctggcgca ccatgatcat tgcgctggga atgtttggcg cggtggtgtg
     2641 gttcgtgtac atccgcggag aataaatttc gttccgccca gattgggtgt tgtcaaacct
     2701 gctcttgaac aggcacagat gctgcagctt tttcgttgat attaaccgca agctccgcaa
     2761 gttcgccatt caacacgcgc acagcttttt cgcggtccag ttcattttcc caacgggcga
     2821 tgacgacggt cgcgacgccg ttgccaatca tatttgtcat cgcgcgcgcc tgggccatga
     2881 accaatccac acccagaatg atgaccatgc cttcgacggg aatggtgtgc atggacgaaa
     2941 gcgtcgcagc cagcgtgatg aaagagcttc ccaccaccgc cgcggcgcct ttggaagtga
     3001 gcagacaaac aaacagcacc gcgagttcct ggcccaaggt cagatgagta ttcgtcgctt
     3061 gagcaatgaa caacgcgccc attgtcaaat aaacagagga accgtcgagg ttgaatgagt
     3121 aacccgatgg cagaacaatt ccaaccaggg gctttgaaca gccgagttgc tcaagcttcg
     3181 ccaggagccg gggaaaaacc gcttcggaag atgccgtgcc gaaaacaatg aaaaattcgt
     3241 cgcgcagata atgcagcatt ttccagatgc tcagtccgtt cagacgcaac aatgaaccga
     3301 ggcagattac cacaaagaaa atgcaggtga gataaacaca cgccatcaac ttggccagcg
     3361 agacgagcga gccgacgcca aattttgcga tggtgaaact catcgctccg aaaactccca
     3421 atggagcaaa tttgatgatc atcgcaatca tccgcatcag cgccttggag acttgttcga
     3481 gggcccggat gatggggcgg ttcgcttcgc ccattcccga cattgccatc ccaaaaatga
     3541 ccgagaagaa gagcacttgc aggatttctc catcagcaaa agagcccaca acacttttgg
     3601 ggatgacgtt cagcaaaaat tcgaccgcgc catgcggcgc cttggctgcg gcgagagtgg
     3661 attgcaaatc ggagatgttc agttggctga tgtccgcgtt catcccggcg ccgggttgaa
     3721 aaacttttgc aacagtccaa ccaatggcaa gcgccagcgt ggtcatcacc tcaaaataaa
     3781 ggaacgattt caaaccgatg cgcccgactc gtttcaggct gtccatcccg gcgatgccga
     3841 ccacaacatt ggtgaagatg attggcgcaa tcatcatccg gatcagcttg atgaa
//
LOCUS       DASZMM010000215         4163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3107130, whole genome shotgun sequence.
ACCESSION   DASZMM010000215 DASZMM010000000
VERSION     DASZMM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3107130"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..2030
                     /gene="thiC"
                     /locus_tag="VG754_05105"
     CDS             108..2030
                     /gene="thiC"
                     /locus_tag="VG754_05105"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HWF18620.1"
                     /translation="MIATKESFEPQSGDPLPNSAKVHVRGKLHPDLRVPMREIKLSDT
                     KTFSGQIETNAPVRVYDCSGPWGEQDFKGDVTQGLPRLREQWIRNRGDVEEYDGREIK
                     PMDNGYLSRNHAEYASQSERNRLLQFPGHKRKPLRAAKGHPVTQLWYARQGMITPEME
                     FIAIRENMGREAAMELVENKNGDRRVLNQQHPGQSWGARVPNHITPELVRDEVARGRA
                     IIPANINHPESEPMIIGRNFLVKINANIGNSAVASSIEEEVEKMRWATKWGADTVMDL
                     STGKNIHATREWILRNSPVPIGTVPIYQALEKVGGKAEELTWESYRDTLIEQCEQGVD
                     YFTIHAGVLLRFIPLTARRATGIVSRGGSIMAKWCLAHHQESFLYTHWDDICEIMAAY
                     DVSFSIGDGLRPGSIADANDEAQFAELKVQGDLTKRAWAHGVQVMNEGPGHVPMHMIQ
                     ENMEKQLEWCSEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHL
                     GLPNKKDVKDGVITYKIAAHAADLAKGHPGAQYRDNALSKARFEFRWEDQFNLSLDPE
                     TAREFHDETLPQEGAKAAHFCSMCGPHFCSMKITEDVRKYAAEQGIAEDEAIKKGMEE
                     KSKEFVEKGAEVYAKA"
     gene            2049..2561
                     /locus_tag="VG754_05110"
     CDS             2049..2561
                     /locus_tag="VG754_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18621.1"
                     /translation="MAINEPSANLPLADVLRAANDLMAAGLIEDWALGGALAAIYYIE
                     PFATYDANIFFIPKDKELTAGIPAIYTHLQSQGWQVEREHLMVRGFPVQFLVGHGLTA
                     EAVREAQIIEFEGVPAKIFRAEHIIAIAASVGRHKDLARIEQMLQQADLDKTMLKDIL
                     ERHNLKLPKL"
     gene            2704..2844
                     /locus_tag="VG754_05115"
     CDS             2704..2844
                     /locus_tag="VG754_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18622.1"
                     /translation="MKITEDVRKYAAEQGIAEDETINEMKERSKEFVEKGAKGLQMLI
                     LA"
     gene            2841..3254
                     /locus_tag="VG754_05120"
     CDS             2841..3254
                     /locus_tag="VG754_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18623.1"
                     /translation="MNSASQDFPHHLGVLRERMLHPTDYEQAVYYFLEEFAGDSAFVS
                     ASDKETMPHLVAVLGHVVSKALEKTVELEGALVSYLREHRFVHGNAQADGRIVLFFYF
                     EQADTGMVMIIPGIRGQGEMARFHLTGGLPDPQKN"
     gene            3280..3780
                     /locus_tag="VG754_05125"
     CDS             3280..3780
                     /locus_tag="VG754_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18624.1"
                     /translation="MHHYWHTFVELLATPFHQITSVWGIVPIYFALFLNELTSDKADF
                     RTALQTGFSFLWAAAQWIYPYFRPRETGIPHVEWNAMLPVNLAVTGLVVAFGAMALYS
                     GILRRYPKYCSFLGHTRFANYFMITIYPMQAHYLAWTWERLAAIVIFAVPVWLLLHFG
                     LKPVRR"
     gene            3879..>4163
                     /locus_tag="VG754_05130"
     CDS             3879..>4163
                     /locus_tag="VG754_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18625.1"
                     /translation="MKSIQGYHLIKPEDLFWRPSNLMQIPNADFLERTGSENPEVCGW
                     SSTQPRSTEASEAGSTASKEAENVPISLDAAPGVWERRERLTILAGGLFGG"
BASE COUNT          975 a         1130 c         1209 g          849 t
ORIGIN      
        1 ccggccggag gccaaagcgc ttcgcaccct tcaatgacat tcctgtcgca atgaattggc
       61 atgcaactga tggccatgaa ccatcaccgc acatcgacta cgatattatg attgcgacca
      121 aagagtcctt cgaaccgcaa agcggcgatc cgctccccaa ttccgccaag gtccacgtgc
      181 gcggcaaatt gcatcccgat ttgcgcgtgc cgatgcgcga aatcaaattg agcgacacga
      241 aaactttcag cggccagatt gaaaccaacg cgccggtgcg cgtttatgat tgcagcgggc
      301 cttggggcga gcaggatttc aagggcgacg tcacgcaagg tttgccgcgc ttgcgcgagc
      361 agtggatacg caaccgcggc gatgtcgagg aatacgatgg gcgcgaaatc aagccgatgg
      421 acaacggcta tctttcgcgc aatcacgccg aatatgccag ccagtccgag cgcaaccggc
      481 tgctgcagtt tcccggccac aaacgcaagc cgttacgcgc cgcaaagggc catcccgtca
      541 cgcaactttg gtacgcgcgc cagggcatga tcacgcccga aatggaattc atcgccatcc
      601 gcgaaaatat ggggcgcgaa gccgcgatgg aactggtcga aaataaaaac ggcgaccgcc
      661 gcgttttgaa ccagcaacat cccggccaat catggggcgc gcgcgttccg aaccatataa
      721 cgcccgaatt ggtccgtgac gaagtcgcgc gcggtcgcgc catcattccc gccaacatca
      781 atcatcccga aagcgagccg atgatcatcg ggcggaattt tttggtgaag atcaacgcga
      841 acatcggcaa ttcagccgtt gcgtcaagca tcgaggaaga agttgaaaaa atgcgttggg
      901 cgaccaaatg gggcgcggac acggtaatgg acctttccac cggcaaaaac atccacgcga
      961 cgcgcgaatg gatcttgcgc aattcgccgg tgcccatcgg cacggtgccg atttatcagg
     1021 cgctcgaaaa agtcggcggc aaggccgagg aattgacctg ggaaagttat cgcgacacgc
     1081 tcatcgagca atgcgagcag ggcgtggatt atttcacgat ccatgcgggc gtgttgctgc
     1141 ggttcatccc gctgaccgcg cgacgcgcga cgggcattgt ttcgcgcggc ggcagcatca
     1201 tggccaaatg gtgccttgcg catcatcagg aaagttttct ctacacgcat tgggacgaca
     1261 tttgcgaaat catggccgcg tacgacgtga gcttcagcat cggcgacggc ttgcgtcccg
     1321 gttcaatcgc cgacgcgaat gacgaagcgc aatttgctga actcaaggta cagggcgatt
     1381 tgacgaaacg cgcttgggcg catggcgtgc aggtgatgaa cgaaggcccc ggccatgtgc
     1441 cgatgcacat gatccaggaa aacatggaga agcaattgga atggtgcagc gaagcgccgt
     1501 tctacacgct cggcccgctc acgacggaca tcgcgcctgg ttacgaccac atcaccagcg
     1561 gcatcggcgc ggcgatgatt ggttggtatg gttgcgcgat gctttgctac gtcacgccca
     1621 aggaacatct cggtttgccg aacaagaagg acgtgaaaga cggcgtcatc acctacaaga
     1681 tcgccgcgca cgccgcggac ctcgccaagg gccatcccgg cgcgcaatac cgcgacaacg
     1741 cgttgagcaa ggcgcgcttt gaattccgct gggaagacca gttcaatttg agcctcgatc
     1801 cggagaccgc ccgcgaattt catgatgaaa ccttgccgca ggaaggagca aaggcggcgc
     1861 atttttgcag catgtgcggc ccgcattttt gctccatgaa aatcaccgaa gacgtccgca
     1921 aatacgcggc ggaacagggg attgccgaag acgaagccat caaaaaaggg atggaagaaa
     1981 agtcgaagga gtttgtggaa aagggcgcgg aggtttacgc gaaggcatga gtctgatact
     2041 tgatgatcat ggcgatcaac gaaccatccg cgaatcttcc attggccgat gtattgcgcg
     2101 ccgcgaatga tttgatggca gcggggttga ttgaggactg ggcgctcggc ggcgcgctgg
     2161 cggcgattta ctatattgag ccgttcgcca cctacgatgc caatattttt tttattccga
     2221 aagacaagga attgaccgcc ggtatcccgg ctatttacac gcacctgcaa tcgcaaggct
     2281 ggcaggtcga gcgcgaacat cttatggtgc gcggatttcc ggtgcaattt ctggttggcc
     2341 acgggttgac cgcagaagca gtccgagaag cgcaaatcat cgaattcgaa ggcgtgcccg
     2401 caaaaatctt tcgcgccgaa cacatcattg ccatcgccgc aagcgtgggc cggcataaag
     2461 acctggcccg gatcgaacag atgctccaac aggctgacct cgacaaaacc atgttgaagg
     2521 atattctgga acggcataat ttgaaactgc cgaagctatg aaagcgcaga tgcgtcaaaa
     2581 actggctcgc gaagattttg cggaaaaaat ccgcaaggtc gggcagttga tccggttggc
     2641 aaaaacattt ccgcggcgat cctccacgat ttcccgccaa acgccggctc gcacttctgc
     2701 tccatgaaaa tcaccgaaga tgtccgtaaa tacgcggcgg agcaggggat tgccgaagac
     2761 gaaaccatta acgagatgaa agaaaggtcg aaggagtttg tggaaaaggg cgcgaagggg
     2821 ttacaaatgt tgattctggc atgaattccg catcacaaga ttttccgcac caccttggcg
     2881 tattgcgcga acggatgttg catccgacgg attacgagca ggcggtttat tattttttgg
     2941 aagagttcgc cggcgactcg gcgtttgtca gcgcgtcgga taaggaaacc atgccgcatc
     3001 tggtcgccgt attgggccat gtggtttcca aggcgctgga aaagactgtg gaattagagg
     3061 gcgcgctcgt gtcgtatctg cgggaacacc ggtttgtcca cggcaacgcc caggcggacg
     3121 ggcgcatcgt gctgttcttc tattttgagc aggcagacac gggcatggtg atgatcattc
     3181 ccggaattcg aggtcaaggc gaaatggcgc gcttccatct caccggcggc ttgcccgatc
     3241 cgcaaaagaa ttgatttcaa cccctcgtta agcaaaagct tgcaccatta ctggcacacg
     3301 tttgtcgagt tgctcgcaac tccgtttcat cagatcacgt ctgtttgggg gattgtgccg
     3361 atctacttcg cgctgtttct caatgaactg acgtcagaca aggcggattt taggacggcg
     3421 ctccagaccg ggttcagttt tctgtgggcc gcggcgcaat ggatttatcc ttacttccgg
     3481 ccgcgcgaaa caggcattcc acacgttgaa tggaacgcca tgctgccggt caacctcgcc
     3541 gtcaccggac tcgtggtggc gtttggggcg atggcattgt acagcggcat cctgcggcgg
     3601 tatccgaagt attgctcatt tctcggccac acccgcttcg ccaattattt catgatcaca
     3661 atttacccga tgcaggcgca ttatctcgcg tggacttggg aacgtctcgc ggcgattgtg
     3721 atattcgccg tgcccgtttg gctgttgctc catttcggcc tcaagccggt gcggaggtga
     3781 atatgcagca gcagcagttt gggcggcgtg gcagcgccgc cctaccatga ttgattcggt
     3841 agggcaaagc tgccgctttg accattcgtc accaaattat gaaatccatt caaggttacc
     3901 atctgatcaa gccggaagac ctgttctgga ggccatccaa cctgatgcaa attccgaatg
     3961 cggattttct ggagcgcacg gggagcgaaa atcctgaagt ctgcggctgg tcttcgacac
     4021 agccgcgctc cacagaagct tccgaggctg gctcgaccgc atccaaagaa gctgagaatg
     4081 tgccgatttc tttggacgcg gctcccggtg tttgggagag gagggaacgg ctaacgattc
     4141 ttgccggcgg cttgtttggc ggc
//
LOCUS       DASZMM010000216         6806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2561314, whole genome shotgun sequence.
ACCESSION   DASZMM010000216 DASZMM010000000
VERSION     DASZMM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561314"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VG754_05135"
     CDS             <1..1054
                     /locus_tag="VG754_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF18626.1"
                     /translation="HFGPETKAKSDIAGNIGLVSLHLSKPPGREVVVTLGSRVGFGLK
                     SDDDKSFSVAEGTRFVFNDENWNQPALAAIQLDPKVKNAVEGLMEIRSGNLPFAWSVT
                     FFLLAGMFLVFCVYHRIILPYPVSDKPGSTQSLAMFWTEFFRTFAAFFKKQKIVRLLL
                     FLLLYRFAEAQLVKMVVPFLLDAREAGGLALTTSQLGFVYGTVGIAALTCGGLLGGMV
                     AAKNGLKFWLWWMVLAIHLPDAVFVYLAYTMPDNFWVINACVAIEQFGYGFGFTAYML
                     YMIYIARGEHATAHYAICTGFMALGMLLPGMFSGWLADIIGYQHFFVWVLLSTIPGFL
                     VVGLIPLDAEFGKKTA"
     gene            complement(1426..2982)
                     /locus_tag="VG754_05140"
     CDS             complement(1426..2982)
                     /locus_tag="VG754_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18627.1"
                     /translation="MKWLRWLACLFLFCVVGTSRAHIGSPDVFYEGAIGPYPAHVTIR
                     MPVVVPGRAQILVRTDTNSPVEVSFRPLFVGSAVTNAPPADIGHLVPGETNLYTGDLW
                     LMTFGGYSIEVKMRGAAGEGAVEIPVNSVALKQMPMPRAMGIILSALGVLLVLGGAAI
                     VKGAASDSTIAPGVAAGKRERRKGWIAGSVTLFIVTGALVLGALWWKLDERQFREQLN
                     STNPPKLSAETRVEGSQRILRLTLGDAALDSDGLLRLLPDHDKLVHLFLIRQDARDAF
                     AHLHPIRKGNAAFEVALPPLPAGRYDIFCDLTLENSGLSTTATNVVELPPPVAENVSF
                     QRIKILDADPDDSWAASPNATLPPMGTATNAVFQFADGGRVVWKAHGPLRARQDAGLQ
                     FEVTDAAGNPARLEPYMGMISHAAVLRADNGVFAHLHPAGNFSMAAQRFYESKLAVEN
                     AACAPPGTSLPANAEHSIAGHGNHISSGENSTISLPYEFPTAGNYRVWVQIKTGGRVL
                     TAVFDAMVSG"
     gene            complement(2979..4136)
                     /locus_tag="VG754_05145"
     CDS             complement(2979..4136)
                     /locus_tag="VG754_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18628.1"
                     /translation="MTVDSVASGLTHWPGYLMALAATSILIGILWDISWHMSIGRDTF
                     WTPAHMAIYFGGALAGCVGGWLAVKHTFFPTREERDASVNLLGARAPLGAWVAIWGAI
                     AMLTSAPFDDWWHNAYGLDVKIISPPHTVLGLGMLAVALGALLMTLARQNRESNGVGS
                     GMFIYMGAVFLTFGTVFITEFTFPNMQHGFQCYKWCSWMLPIRLVTMGRAGRISWPAT
                     RVAAVYMLLQCLMVWILPLFPAQPKLAPIFNPVTHMVPPLFPLLLVVPAFGIDLFLRN
                     AAGDDRGWRRIGRALAIGAIFLSLLLVTQWFFSEFMLSSHAENWFFAGDRHWSYGADP
                     GSWQKEFWSLDKSASDNQIITPARLIKCWMIASLMSWVALLWGGWMRKVKR"
     gene            complement(4276..5088)
                     /locus_tag="VG754_05150"
     CDS             complement(4276..5088)
                     /locus_tag="VG754_05150"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HWF18629.1"
                     /translation="MDFNFNEWLELILRWAHVFAGIMWVGATFYFTWLDGRFTELEKK
                     ASDGTDAEKFVWMVHSGGFYQVEKQKRPKLMPSTLHWFRWEAALTWLTGFLLFAYMYY
                     HGPYLVSSEDSLISKGAAIGLSLGMLIAGWVAYDLVWRFCKQEMLGMVICYALIVGAA
                     WVACHYFTGRGAYLQVGAMMGTIMAANVWMGILPAQRRMVAALKEGREPDLTEGARAK
                     ARSKHNTFIVIPVVFTMISNHYPGTYGSKYNWLILSTLVLVGWVAAKIIRRA"
     gene            complement(5159..5752)
                     /gene="pgsA"
                     /locus_tag="VG754_05155"
     CDS             complement(5159..5752)
                     /gene="pgsA"
                     /locus_tag="VG754_05155"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWF18630.1"
                     /translation="MNLPNKLTVSRLALTAAFLAVIFSPRVQLRETVALVLFSVASLT
                     DYFDGKIARQRKLITNFGILMDPLADKILVCSAFIAFVGLNWMPAWMVVIIVARELAI
                     TGLRLLAASKNLVLAAERYGKHKTISQIVAIIAILVQHSYNDWGRVGWAVFGFPAFGM
                     PWVDRFAPLAIWVSVVLTFVSGALYLWRNRQLYLDDL"
     gene            5982..>6806
                     /gene="hisD"
                     /locus_tag="VG754_05160"
     CDS             5982..>6806
                     /gene="hisD"
                     /locus_tag="VG754_05160"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWF18631.1"
                     /translation="MKVIRHTDADFAERLRQLTAASSLFDPVIEERARGIVEAVKTRG
                     DAALLELTERFDGAKLTAEQLWVSAPEMLAASLRADESLRAAVATADKNIAHFSRKSL
                     RKNWSARNGQGAKVGEKFDAFQRVGIYIPGGTAPLVSTALMTIVLARVAGCKEIVICT
                     PCGADGSINAALLFAARAAGATEIYRVGGAQAIAAMALGTETIRRVQKIFGPGNAYVV
                     AAKRLLFGHVAIDLLPGPSEVLVLADDTANPKFAAADLLAQAEHGSGHERVWLVTTS"
BASE COUNT         1538 a         1983 c         1889 g         1396 t
ORIGIN      
        1 gcatttcggg cctgaaacca aggcgaaaag cgacatcgct ggaaatatcg ggctcgtttc
       61 gctgcatctt tccaaaccgc ccgggcgcga agtggtggtg acgctcgggt cgcgcgtcgg
      121 cttcggcctc aaatcggatg acgacaaaag tttttccgtc gccgaaggaa cgcgattcgt
      181 ttttaacgac gaaaattgga accagcccgc gctcgcagca atccagttgg accctaaggt
      241 gaagaatgct gtcgagggcc tgatggaaat ccgttccggc aacctgccat tcgcatggtc
      301 cgtcacgttt tttttattgg cgggaatgtt tttggttttc tgcgtctatc atcgcatcat
      361 cctgccgtat ccggtgagcg acaagccggg cagcacacag tcgttggcga tgttttggac
      421 agaatttttc cgcacgttcg cggcgttctt caaaaaacaa aaaatcgtcc ggctgctgct
      481 ttttctgttg ctttaccgtt ttgccgaggc gcaactggtg aagatggtcg tgccgtttct
      541 gctcgatgcg cgagaagccg gcggattggc gctcacgacg agccagcttg gttttgtgta
      601 cggcaccgtg ggcattgcgg cgctgacatg cggcgggctt ctgggcggaa tggtggcggc
      661 gaaaaatggg ttgaaattct ggctctggtg gatggtgctg gcgatccatc tgccggacgc
      721 agtgttcgtc tatcttgctt atacgatgcc ggacaatttc tgggtcatca atgcctgcgt
      781 ggcgattgaa caattcggtt acggttttgg tttcaccgct tacatgcttt acatgattta
      841 catcgcgcgc ggcgaacacg ccacagcgca ctacgcgatc tgcacggggt tcatggcgtt
      901 gggaatgctg ctgcccggca tgttcagcgg ctggctggcg gacatcatcg gctaccagca
      961 tttttttgta tgggtgctgc tctcgactat tcccggtttt ctcgttgtgg gtttgatccc
     1021 gctcgacgcc gagttcggca aaaaaacggc ttaaaaagag tttaatcaac gtcttcataa
     1081 tgctgcgccc cgggatgcct tgttattatt tatactgtcc cgaaaggaat agctgctcta
     1141 cttaggaagg aagttgagca tgtccttgat ttgcacggca tgaaatttgg agtgggacgc
     1201 ttcccataat agctactttt taaaaaaatt tctaacgaat ggaaattttc gggtaaggcc
     1261 atcgaataaa gcagcgattc gtccgaatgt ataaaacctc cggccagtaa accctcacgc
     1321 agggaaaaat taaccctcat gcggggaagt cccggccgaa ggaagtctgc ctaaacctaa
     1381 ggcaatcgcg cgcggatgtc acgcgtcaat cgtatatcgt aattctcacc cgctcaccat
     1441 cgcgtcaaaa accgccgtca acacgcgtcc accggttttg atttgcaccc agacgcgata
     1501 attgcctgcg gtcgggaatt cgtatggcaa cgatatcgtc gaattctctc ccgacgaaat
     1561 atgattgccg tgaccggcga tggaatgctc cgcgttggca ggcaggcttg ttcccggcgg
     1621 ggcacaggcg gcgttttcaa ccgccagttt gctttcataa aagcgttgcg ccgccatgga
     1681 aaaattgccc gcaggatgca ggtgcgcgaa cacgccgttg tccgcgcgca acacagccgc
     1741 gtgggaaatc atccccatgt aaggctccag ccgcgcggga ttgccggcgg catccgtgac
     1801 ttcgaattgc aacccggcat cctgccgcgc gcgcaacggg ccgtgcgcct tccaaaccac
     1861 gcgcccgcca tcggcaaatt ggaaaacggc attcgtcgcc gttcccatcg gcggcaaagt
     1921 ggcgttcggc gaagcggccc atgaatcgtc ggggtcggca tcgaggattt taatccgctg
     1981 aaaactcacg ttttcagcta cgggaggggg aagttcgacc acgttggtcg cggttgtgct
     2041 caggccggaa ttttcgagcg tcaggtcgca gaaaatatca taacgcccgg cgggcaatgg
     2101 cggcagcgcg acttcaaatg ccgcgttgcc tttgcgaatc ggatgcagat gcgcgaacgc
     2161 gtcgcgcgcg tcctggcgaa tgagaaaaag atgcacgagc ttgtcgtgat cgggcaacag
     2221 ccgcagcaag ccgtcggaat caagcgcggc gtcgcccaag gtcagtcgga gaatgcgttg
     2281 cgagccttcc acgcgcgtct cggcggacaa tttgggagga ttggtggaat tcaattgctc
     2341 gcggaattgc cgttcgtcca gcttccacca caatgcgcca agcaccagcg ccccggtgac
     2401 gatgaacagt gtgaccgatc cggcaatcca tcctttgcgg cgttcgcgtt ttcccgcggc
     2461 aacacccgga gcgattgtgc tgtcgctcgc ggcgcctttt acgattgctg cgccaccgag
     2521 cacaagcaat acccccagcg ccgataaaat gatgcccatc gcgcgcggca tcggcatttg
     2581 tttgagcgca acagagttca cgggaatttc gaccgcgcct tcacctgccg caccgcgcat
     2641 ctttacctcg atgctgtaac cgccaaaagt catcagccac aagtcaccgg tgtaaaggtt
     2701 cgtttccccg ggaacaagat ggccgatatc cgcaggcggg gcgttcgtca cggcgctgcc
     2761 aacgaaaagc ggacggaacg acacttccac tggcgaattg gtgtcggtgc ggaccaaaat
     2821 ttgcgcgcgt cccggcacga ccaccggcat cctgatcgtc acatgcgcag gatacggccc
     2881 gattgcgcct tcatagaaaa catccggact gccgatgtgg gctcgcgaag ttcccacaac
     2941 gcaaaaaaga aaaaggcacg ccagccaacg gagccatttc atcgcttcac ctttcgcatc
     3001 cagccgcccc aaagcaatgc gacccatgac atcagactgg cgatcatcca gcatttaatg
     3061 agtcttgcgg gcgtgatgat ctgattgtcc gacgccgatt tgtccaggga ccaaaactcc
     3121 ttttgccagc ttcccgggtc tgcgccataa ctccaatgcc ggtctcccgc gaaaaaccag
     3181 ttctccgcgt gcgacgacag catgaattca gagaagaacc attgcgtcac cagaaggagc
     3241 gacagaaaga ttgcgccgat ggccaacgcg cggccaatgc gccgccatcc gcggtcgtca
     3301 ccggccgcgt tgcgcaggaa caggtcgatg ccgaacgccg gcacgaccag caacagcggg
     3361 aagagaggcg ggaccatgtg cgtgacggga ttgaagattg gcgcgagttt tggttgcgcg
     3421 gggaacagcg gcaaaatcca gaccatcagg cattgcaaca gcatatagac cgctgccaca
     3481 cgcgttgccg gccacgagat gcggcccgcg cggcccatgg tcacgagccg gatcggcagc
     3541 atccatgaac accatttgta acactgaaaa ccatgttgca tgttcggaaa ggtaaattcg
     3601 gtgatgaaca ccgtgccgaa cgtgaggaac accgcgccca tgtagatgaa cattccgctg
     3661 ccgacaccgt tcgactcgcg gttttgccgc gccaatgtca tcagcaacgc gcccagcgcg
     3721 acagccagca ttcccaatcc cagcaccgtg tgcggcgggc tgatgatttt tacgtcgagc
     3781 ccgtaagcgt tgtgccacca atcatcaaac ggcgcggaag tcagcatcgc gattgcgccc
     3841 caaatcgcaa cccacgcacc gagcggagcg cgcgcgccga ggagattcac agaggcatcc
     3901 cgttcctcgc gagtggggaa aaaggtgtgt ttcaccgcca gccagccgcc gacgcagccg
     3961 gccagcgcgc cgccgaaata aattgccatg tgcgcgggcg tccagaaagt gtctcgccca
     4021 atgctcatgt gccacgagat gtcccaaaga atgccgatga gaatgctcgt cgcggcgagc
     4081 gccatcagat agccgggcca atgcgtcaat cccgacgcga ccgaatcgac ggtcaactca
     4141 ctttccgacc ggctcgtttt tgaaagcgac aaagacatat gaccttccct gcgcacctat
     4201 gttatgtatg agccataatc ccatcgcact gatacaactc aattacaaat gcaatgggtg
     4261 attataattc gcgtttcacg cgcgtcggat gattttggcc gcgacccaac cgaccaaaac
     4321 cagcgtcgac aggatgagcc aattatattt gctgccgtaa gtgccgggat aatgattgct
     4381 gatcatcgtg aacaccaccg ggatgacgat gaaggtgttg tgcttggaac gcgccttggc
     4441 ccttgcgcct tcggtgagat cgggttcgcg cccttccttc aaggccgcca ccatgcggcg
     4501 ttgcgcggga aggatgccca tccagacatt cgccgccatg attgtcccca tcatcgcacc
     4561 gacctgcaaa tacgctccgc gtccggtgaa ataatggcac gcgacccatg ccgccccaac
     4621 tatcagcgca taacagatca ccatgcccaa catttcctgt ttgcaaaagc gccacaccaa
     4681 atcatacgcg acccagccgg caatgagcat tcccaaactc aatccaatgg ccgcgccttt
     4741 gctgataagc gaatcctccg agctgacgag atacggcccg tgataataca tatatgcgaa
     4801 caacagaaaa ccagtgagcc atgtcaacgc cgcttcccaa cggaaccaat gcagcgtgga
     4861 cggcatgagc ttgggccgtt tctgtttttc gacctggtaa aacccgccgc tgtggaccat
     4921 ccagacaaat ttttcggcgt cagttccgtc ggatgctttt ttttccagct cggtgaaacg
     4981 tccgtcgagc catgtgaaat aaaaagttgc tccgacccac atgatgcccg caaagacatg
     5041 cgcccagcgc aaaatgagtt ccagccactc gttgaaattg aagtccatag atgcaaattt
     5101 tgcatcgcca gagtgtgtac atttcgcggc aggtcaagca ggaagtgctt ttacgccctc
     5161 acaaatcatc cagataaagc tgccggttcc gccacagata cagcgctccg gatacgaatg
     5221 tcagcaccac agaaacccag atggccaacg gagcaaaccg gtcaacccaa ggcatgccaa
     5281 aagcgggaaa tccaaacacg gcccaaccga cccggcccca atcattgtag ctgtgctgaa
     5341 caaggatcgc gatgattgcg acgatttgcg agatggtctt gtgcttgcca tagcgttcgg
     5401 ccgcgagcac caagtttttc gacgccgcca gcagacgcaa cccggtgatg gccagttcgc
     5461 gggcgacgat gatcaccacc atccacgcgg gcatccagtt caacccgaca aatgcgatga
     5521 atgccgagca gaccaaaatt ttgtcggcga gcgggtccat caagatgccg aagttcgtga
     5581 tgagtttgcg ttggcgggca attttgccat cgaaataatc ggtcaggctc gccacgctga
     5641 acagcaccag cgcgaccgtt tcgcgcagtt gcacgcgcgg tgaaaaaatc accgccagaa
     5701 acgccgctgt gagcgcaagc cgggagacgg tcagtttgtt gggcagattc atcgaaacat
     5761 gaattgcaga attaaagacc gaaccggaca tatggtcaaa cctttgtggg cttgatgaga
     5821 ttgaaaacat cgaacgctga acgtccaaca tccaacgccg aaacaacacg gaaccgatgg
     5881 ttatttattg gacgttcagc gttcgatgtt ggttgttcaa tgtttctttc gcagttccgc
     5941 agttttgact tttacccagt cttgccccag tattcgcccg catgaaagtc atccgccata
     6001 cggacgccga ttttgccgag cggctccgcc agctcaccgc ggcgtcgagc ctgttcgacc
     6061 cggtcatcga ggagcgcgcg cgcggcatcg ttgaggcggt aaaaacacgc ggggatgccg
     6121 cgttgctcga attgaccgag cgtttcgacg gcgcaaagtt gacggcggaa caactatggg
     6181 tcagcgcgcc tgaaatgctc gcagcttcgt tgcgcgcgga tgaatcgttg cgcgcagccg
     6241 ttgctacagc ggacaaaaac atcgcccatt tcagccgcaa atcacttcgc aagaattggt
     6301 ctgcgcgcaa tgggcagggc gcgaaggtcg gcgaaaaatt cgacgccttc cagcgggttg
     6361 gcatctacat tcccggcggc acagcgccgc tggtttcaac cgccttgatg accatcgtgc
     6421 tggcgcgcgt ggcgggctgc aaagagattg tcatctgcac cccctgcggc gcggatggca
     6481 gcatcaacgc cgcgctgctt tttgccgccc gcgcggcggg agcgacggag atttaccggg
     6541 tcggcggcgc gcaagccatc gcggcgatgg cgcttggcac cgagacaatc cgccgcgtgc
     6601 aaaagatttt cggccccggc aatgcatacg tcgtcgccgc caagcggttg cttttcggcc
     6661 atgtcgcgat tgaccttttg ccaggcccca gcgaagtgct cgtgcttgcg gatgacacgg
     6721 cgaatcccaa attcgccgcc gccgatttgc tggcgcaagc cgagcatggt tccggtcacg
     6781 aacgcgtctg gctcgtcacc acttcg
//
LOCUS       DASZMM010000217         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3929932, whole genome shotgun sequence.
ACCESSION   DASZMM010000217 DASZMM010000000
VERSION     DASZMM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3929932"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VG754_05165"
     CDS             complement(<1..500)
                     /locus_tag="VG754_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HWF18632.1"
                     /translation="MRLTRDQIQQRVRELGRWFHNLDLDGVQTAPDHFLGDYPRLKWQ
                     TFADAIPEDLEGRSVLDIGCNAGFYSLEMKRRGAGRVVGIDSDPAYLAQARFAAEVSG
                     FEDDVELRQLSVYDVARLGEKFDIVLFMGVLYHLRHPLLALDLIHEHVARDLLVFQTM
                     QRGSNAV"
     gene            complement(530..1438)
                     /locus_tag="VG754_05170"
     CDS             complement(530..1438)
                     /locus_tag="VG754_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWF18633.1"
                     /translation="MWGIIPAAGAGSRIQPLAFSKELLPVGSRLDGAMERPRAVSEYL
                     VERMLRGGVKKICFVISHGKSDILEYYGGKIGPADIAYVVQPRPAGLCDAIFRAVPLI
                     ASDETVAIGLPDTIWFPQDGLCSLPDDALSFLLFPVKHPQFFDAVVMDEKGRVTEIQV
                     KNPHARSCWIWGAFKMPGHVLHELFQLWQTREPRDEYIGTLVNAYLAGGGRAVGVRAG
                     EAYVDVGTLNGYREAIRLLSSPTADAATRSHSRKGGKGAQRPGRIRGKPVLLQHRAHD
                     DSNFSCGSHKRGAEAKIWSSPFIKEP"
     gene            complement(1440..2537)
                     /locus_tag="VG754_05175"
     CDS             complement(1440..2537)
                     /locus_tag="VG754_05175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF18634.1"
                     /translation="MKLVIFGLTISSSWGNGHATIWRGLCRALAKRGHRVVFFERDVP
                     YYAEHRDLLELPGVQLRLYSDWDEVRPLAKQELSDADVGMVTSYCPDGVAASDAVLDS
                     SARLKVFYDLDTPVTLESMTAGKTVSYIGPRGLSDFDLVLSYTGGCALHELKSRLGAR
                     RVAPLYGSVDPESHRPAPSMQAYAADLSYIGTFAADRQRTLESLFIEPARRLPQNRFV
                     IAGAMYPQDFPWTKNIMFVRHLPPSEHPGFYCSSRLTLNVTRAPMAAMGYCPSGRLFE
                     AAACGVPVLSDWWEGLDEFFEPGSEIMAGFRCEDTIAALEMPPEQLARIGRAARERAL
                     SEHTAEHRADDLEAALDAALMREPEHHFMEV"
     gene            complement(2534..3736)
                     /locus_tag="VG754_05180"
     CDS             complement(2534..3736)
                     /locus_tag="VG754_05180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF18635.1"
                     /translation="MRATAENIIWDTPFATPFADAVPKREQGVATPKQRKGIVDDLPS
                     KSLKFVILGLSITSSWGNGHATTYRGLVRELVARGHDVLFLERNLAWYAANRDLPKPP
                     YGRTALYDSLPQLKKRFASAIRNADCVIVGSYVHEGAAVGEWVIQTAQGVTAFYDIDT
                     PVTLSKLVRAEVDYLTCALIPQYQLYLSFTGGPTLERLERDFGSPMARPLYCSVDPSL
                     YFPQSAKLRWDLGYMGTYSEDRQPALERLLLEPAGHWTEGRFVVAGPQFPKQIKWPRN
                     VRRVVHLSPARHRAFYNAQKFTLNITRARMVEAGYSPSVRLFEAAACGTPIITDYWEG
                     LEDFFQPGSEILVARSSKEALKYLRNISEPERRQIGENARARVLAEHTAAHRAVQLEE
                     YVHELIRK"
     gene            complement(3733..>4119)
                     /locus_tag="VG754_05185"
     CDS             complement(3733..>4119)
                     /locus_tag="VG754_05185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF18636.1"
                     /translation="NVRYAGHVYTRDHNAFNCSPLAVLNVNRESMAQYGFSPPTRVFE
                     AAGAGACLITDAWEGIELFLEPDHEVLVAEDGNEVVGHLHSLTADRARAIGQAAMKRL
                     LSEHTYAHRAAQLETVLSGKHEEAFA"
BASE COUNT          931 a         1200 c         1187 g          801 t
ORIGIN      
        1 acggcgttgc tgccccgttg catggtctgg aagacaagca aatcgcgcgc aacgtgctca
       61 tggatgaggt cgagcgccaa caatggatgc cgcaaatgat aaagcacacc catgaacagc
      121 acgatgtcga acttttcgcc cagtcgcgcg acgtcataga ccgaaagctg gcgcagttca
      181 acgtcgtcct cgaagccgga aacttccgcg gcaaaacgcg cctgcgccag gtacgccggg
      241 tcggaatcaa tgcccacgac gcgtcccgcg ccgcgccgct tcatttcgag cgagtaaaat
      301 cctgcgttgc aaccgatgtc caggaccgac ctgccttcga ggtcttcggg aatggcatcg
      361 gcgaacgttt gccatttgag gcgcggataa tcgccgagga aatggtccgg cgcagtttgc
      421 acgccatcga ggtcaaggtt gtgaaaccat cggccaagtt cgcggacgcg ctgctgaatt
      481 tgatcgcggg tcaatcgcat ccggttggaa gaagaaagag tggtcgtgtt cacggttcct
      541 ttatgaatgg agaactccat attttggcct cggcgccgcg tttgtggctg ccacaagaaa
      601 aattcgaatc gtcatgcgcc ctatgctgaa gcaacacagg tttgccgcgg atgcgccccg
      661 gtcgttgcgc gcccttgccg ccctttcgcg aatgagagcg ggtcgcggca tccgccgttg
      721 gcgaactgag cagacggatg gcttcgcggt agccgttcaa tgtgccgacg tccacgtagg
      781 cttcgccggc gcgcacccca accgcgcgac cgccgcccgc cagataggcg ttgaccagcg
      841 tgccgatgta ttcatcgcgc ggctcgcgcg tttgccacaa ttggaaaagt tcatgcagca
      901 catggcccgg catcttgaaa gcgccccaaa tccagcagga acgcgcgtgc ggatttttca
      961 cctggatttc ggtcacgcgg cctttttcgt ccatgacaac ggcgtcgaaa aattgcggat
     1021 gcttcacagg gaacaggagg aacgacaagg cgtcatccgg caacgaacac aatccgtcct
     1081 gtggaaacca gatggtatcg ggcaggccaa tggcgacggt ctcgtccgac gcaatcagcg
     1141 gcaccgctcg aaaaatggca tcgcacaaac ccgccgggcg cggttgcacg acgtaagcga
     1201 tgtccgcagg gccgattttt ccgccgtaat attcaagaat gtccgacttg ccatgtgata
     1261 tgacgaagca aatctttttg acgccgccgc gcagcatccg ctcgacgaga tattcgctga
     1321 cggcgcgcgg gcgttccatc gcgccgtcca ggcggcttcc gacgggcagc aattccttcg
     1381 agaaagccag tggctggatt ctgctgcctg cgcccgcggc gggaatgatg ccccacatat
     1441 caaacctcca taaaatgatg ttccggttca cgcatcagcg cggcatcgag cgcagcctcc
     1501 agatcgtcgg cgcgatgctc ggcagtatgc tcggaaagcg cgcgttcacg ggctgcgcgg
     1561 ccaatgcgcg cgagctgttc gggcggcatc tccaaagcgg cgatggtgtc ttcacaccgg
     1621 aaaccggcca tgatttccga gccgggctcg aaaaattcgt ccaaaccctc ccaccaatcg
     1681 ctgagcaccg gaacgccgca cgccgccgct tcgaacaaac gtcccgacgg gcagtaaccc
     1741 atcgcagcca tcggcgcgcg agtgacgttc aacgtgagtc tcgacgaaca ataaaaaccg
     1801 ggatgctcgg acggcggaag atgccggacg aacatgatgt ttttcgtcca tggaaaatct
     1861 tgaggataca tcgcgcctgc gatgacaaaa cggttttgcg gcagacgccg cgctggttcg
     1921 atgaaaagcg attccaatgt ccgctggcgg tcggcggcaa aagtgccgat gtaagaaaga
     1981 tcggcagcat aggcttgcat ggatggcgct ggccgatggc tctcaggatc cacgcttcca
     2041 taaagcggag cgacacggcg cgcgccaagg cgggatttca attcgtggag ggcgcagcct
     2101 cccgtgtagc tcaacaccag gtcgaagtcg ctcaacccgc gcggaccaat gtaactgaca
     2161 gttttgccgg ccgtcatgct ttccagcgtg accggagtgt ccaaatcata aaataccttg
     2221 agccgcgcgg acgagtcgag caccgcatcg gacgcggcca caccgtcggg acaataggaa
     2281 gtcaccatgc caacatcagc atcggaaagt tcttgtttgg cgagcggacg gacttcatcc
     2341 caatccgagt aaagccgcaa ttgaacgccg ggaagttcaa gaaggtcgcg atgttccgcg
     2401 taatagggaa cgtcgcgttc gaaaaaaacg acgcgatgtc ctcgttttgc aagcgcgcgg
     2461 caaaggccgc gccaaatcgt ggcatggccg ttgccccacg aagaactgat tgtcaggcca
     2521 aaaatcacca gtttcatttt ctaatcaatt catgaacgta ttcttcaagt tgaacagcgc
     2581 gatgggccgc agtgtgttcc gcaaggacgc gcgccctcgc attttccccg atttgccgac
     2641 gttccggctc cgaaatgttc cgcaaatatt tcagcgcctc cttgctggag cgcgcgacga
     2701 ggatttcact tcccggctgg aaaaaatcct ccagtccctc ccaataatcg gtgatgatgg
     2761 gcgtgccgca cgccgcggct tcgaacaatc tcacgctcgg cgaataaccg gcttccacca
     2821 tgcgggcgcg agtgatattg agcgtaaatt tttgcgcgtt gtagaaagcg cgatgccgcg
     2881 cgggagaaag atgcacaacg cgccgaacgt tgcgcggcca tttaatctgc ttgggaaatt
     2941 gcggacccgc cacgacaaaa cggccttcgg tccaatgccc ggccggttca agcagaagcc
     3001 gttcaagcgc cggttgccgg tcttcgctgt aagtgcccat gtaacccagg tcccatcgga
     3061 gcttggcgga ttgcggaaag tacaacgacg gatccacaga acaataaagt ggccgcgcca
     3121 ttggcgaacc aaaatcgcgt tccaaccgtt caagagttgg gccgccagtg aacgaaaggt
     3181 aaagttggta ctgcggaatc aaagcgcagg tgagataatc cacttccgcg cgcaccagtt
     3241 tggaaagtgt gaccggcgtg tcgatgtcat aaaaagccgt aacgccttgg gcggtttgaa
     3301 tcacccattc tcccaccgcc gcgccttcgt ggacgtacga accgacgatg acgcagtccg
     3361 catttcggat cgccgacgcg aatctctttt tgagttgcgg caggctgtca tagagagcgg
     3421 tgcggccata aggcggtttg ggcaaatcgc gattggccgc gtaccaggcg agattgcgtt
     3481 ccaaaaacaa aacatcgtgg ccgcgcgcga cgagttcgcg gacaagcccg cgataggtcg
     3541 tggcatgccc gttgccccat gaggaagtga tggacaatcc aagtatgaca aatttgagcg
     3601 actttgatgg caaatcgtca acgatgccct ttcgctgttt cggggtggca actccttgtt
     3661 cgcgctttgg aacagcgtct gcgaaaggtg tcgcaaaagg tgtgtcccaa atgatatttt
     3721 ccgcagttgc tctcatgcga atgcctcctc gtgtttgccg gaaagcactg tttccaactg
     3781 ggccgcgcga tgggcataag tatgttccga aagcaggcgt ttcatcgccg cctggccgat
     3841 ggctcgcgcg cgatcagcgg tcaaagagtg caaatgtcca accacttcat tgccgtcctc
     3901 ggcgacaaga acctcatgat ccggttccaa aaacaattct atcccttccc atgcgtcggt
     3961 gataaggcac gcgcctgcac ccgccgcttc gaaaacccgc gtgggcggcg aaaagccgta
     4021 ctgcgccatg ctctcgcggt tcacattaag gacggcgagc ggcgaacaat tgaacgcatt
     4081 atggtcgcga gtgtaaacgt ggcccgcata acggacgtt
//
LOCUS       DASZMM010000218         4866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3643997, whole genome shotgun sequence.
ACCESSION   DASZMM010000218 DASZMM010000000
VERSION     DASZMM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643997"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..1224
                     /gene="fabG"
                     /locus_tag="VG754_05190"
     CDS             481..1224
                     /gene="fabG"
                     /locus_tag="VG754_05190"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWF18637.1"
                     /translation="MSQLANQIAVVTGAGRGIGRAIALKFAAEGADVACVSRTAENSE
                     KVAGEVRALGRKAWAYAVDVADAASVNAAAEKILADAGRVDILVNNAGVTRDGLMMRM
                     SDEDWDTVLNTNLKGAFHFTRALSRSFIKQRSGRIINVASVIGLIGNAGQCNYAASKA
                     GLIGLTKSVARELASRGISVNAIAPGFIETDMTAGLKEEWKAELLKKIPLNCLGQSED
                     IAHAALFLASPGSRYVTGQVLTVDGGMVM"
     gene            1527..1772
                     /gene="acpP"
                     /locus_tag="VG754_05195"
     CDS             1527..1772
                     /gene="acpP"
                     /locus_tag="VG754_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWF18638.1"
                     /translation="MAEKSIEQRVKEIIVEQLGVNADQVTPDAKFIEDLGADSLDTVE
                     LVMALEEEFGHEIPDEQAEKLQSVGDVIKYIEDVQQK"
     gene            1838..3097
                     /gene="fabF"
                     /locus_tag="VG754_05200"
     CDS             1838..3097
                     /gene="fabF"
                     /locus_tag="VG754_05200"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWF18639.1"
                     /translation="MPLNWTERRVVVTGLGVISSLGQHIEPFWKHLLEAQCGISKISS
                     FDASAFDCQIAAEIKDFVPGPGFPSPKELKRTDRFAQFGICAGYQALMDSGLDLEKVN
                     RDEIGVFIGSGIGGLKTVEEQHSVLLERGAKRISPFMIPMLILNMASGLFSMYFKLRG
                     PSFATCSACATSTHAIGEAWRTIKMGDATAIFAGGTEATIVPMGIGGFCAMRAMSTRN
                     DDPQHSSRPFDKDRDGFVMGEGAGVLVMEELEHAKARGAKIYCEIVGYGNTSDASHIT
                     APAPEGEGAARCMKMALRTGGLNPTDVSYVNAHGTSTPQGDICETQAIKTVFGEHARK
                     LAVSSTKGATGHMLGAAGAVEMAVCAKAIQTDTVPPTINYTTPDPECDLDYVPNAARQ
                     VRVNAVLNNSFGFGGHNATIAAKKFAE"
     gene            complement(3121..3726)
                     /locus_tag="VG754_05205"
     CDS             complement(3121..3726)
                     /locus_tag="VG754_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18640.1"
                     /translation="MKRKKIIVASCMALIIGLAIAIWWEKFGGQPTLVFKGYDKLNYS
                     SDLLRAKLELRNTTKQPIWFLYCGQRHALESEFMQRCFLSGIIGRTNRFDSMLYIEPS
                     ENSGGCSARLAKLMPNESCMLEWTLSPRSSQKHLGICYYYGDFKDNSDFLSNCWVQPL
                     FTNASFKEKIEFYCKKEFRSQRVVYCPEPFSYEEGASTLRP"
     gene            complement(3809..4624)
                     /gene="larE"
                     /locus_tag="VG754_05210"
     CDS             complement(3809..4624)
                     /gene="larE"
                     /locus_tag="VG754_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWF18641.1"
                     /translation="MNKLDQLRALLRSYGSCLVAYSGGVDSVFLARMASEVLGEKSLA
                     AIADSPSLPRRELEEALTIAEKFGFPVKVVQTQEFSNPQYLANPDNRCYFCKHELFTE
                     LEPLARTGGFAVIAYGENASDVGDHRPGAKAASEFQVRAPLKEAGLTKAEIREFSAQL
                     GLPTADKPQMACLSSRIPYGEAVSPEKLRMIEQAEYVLRDFGFYDVRVRHHELKQGQM
                     ARIEVGPSELSKFVANDTAQRVATALKKIGYLHVTLDLLGYRRGSVNEAVKVA"
BASE COUNT         1154 a         1281 c         1379 g         1052 t
ORIGIN      
        1 agacgagttt ttggcgtgta ggacaaaaac tttgttcctt acaagaaaaa aggggtgttt
       61 ttctgcacag gcaggcaggc ggagcctgca ccctggcaag cagagcctgc acccagcgga
      121 aaagaccatg aaaccaggat ggcggaccgc ggctgtgcgc gaagcgccag ccgcagcaac
      181 gtgccaaaac atcgagtgtt ccaagctgct gcggctggtt ctgcgaacac agccgcgctc
      241 cgttcctatt caaagccacg gatggacacg gattaaaacg gagaagacca gacacgaatt
      301 ttcacgaatt gatacgaatt cgctcaaaat gattcccatt tgtgaaaatt attgtcaatt
      361 cgtgtcgttt cttcggggtt tgtgcagggc gcgtccccaa gcattcgcat cggctcaaaa
      421 aattccaaaa ataatactgt tcatcggggg cgttctcgca aaaaatcgct gttcaactat
      481 atgagccagc ttgccaatca aattgccgtg gtcacgggcg cggggcgcgg catcggacgc
      541 gccatcgcct tgaaattcgc cgccgagggc gcggatgtcg cctgcgtttc gcgcacggcg
      601 gaaaattcgg aaaaagttgc cggtgaagtc cgcgcgctcg gacgcaaagc gtgggcttat
      661 gccgtggatg tggccgatgc cgccagcgtc aatgccgccg ccgaaaaaat ccttgccgac
      721 gcagggcgcg tggacatttt ggtgaacaat gccggtgtga cccgggacgg cctgatgatg
      781 cggatgagcg acgaagattg ggacacggtg ctgaacacca atttgaaggg cgcgttccat
      841 ttcacccgcg cgttatcgcg gagcttcatc aagcaacgtt ccgggcgcat catcaacgtg
      901 gcgtcggtga tcggcttgat tggcaatgcg ggccagtgca attacgccgc gagcaaggcg
      961 gggctcatcg gattgaccaa atccgtggcc cgcgagctgg cttcgcgcgg gattagcgtt
     1021 aacgccatcg cgccggggtt catcgagacg gacatgacgg ccgggttgaa ggaggaatgg
     1081 aaggccgaat tattgaagaa aattccattg aactgccttg ggcaatccga ggatattgct
     1141 catgcggcgt tgtttctggc cagtcccggc tcccgttatg tgacgggaca ggtgttgacc
     1201 gtcgatggcg gcatggtgat gtagaatgat caagtcatct tataaatcaa gaacggagcg
     1261 cggctgtgtc cgcaggacca acggcagcag cttggaactc gataacctgc tgcggctggc
     1321 gcttcgcgca cagccgcagt ccacaataga tgatgtaaaa ttcacgctgg acgcgccgca
     1381 agagagccgt tacagaaggt gagacttatt ggattgcaac ggacaaataa aacgcaaaaa
     1441 tgaaacactt tttcacgatt cagggcttga cgctttctgg aacgtgcctt agacacacac
     1501 tacaaaatac agaaacggag taacccatgg cagaaaaatc aattgagcaa cgagtaaaag
     1561 agatcatcgt ggaacaactt ggggtcaacg cggaccaggt cacgcccgac gccaagttca
     1621 ttgaagatct cggggcggat tctctcgaca ccgtcgaatt ggtgatggca ttggaagagg
     1681 aatttggcca tgagattccg gacgagcagg cggaaaaact ccaaagcgtg ggcgatgtca
     1741 tcaagtacat cgaagacgtc cagcaaaagt agttgccaca cagacggatt cggggcagtt
     1801 tacgctggcg tcagctcaag ctgccccgtc tatttttatg ccattgaact ggactgaacg
     1861 cagggtggtt gtcaccggct tgggagtgat ttcctcgctg gggcagcaca tcgaaccttt
     1921 ttggaaacac ttgctggaag cccaatgcgg catcagcaaa atcagttcgt tcgatgcttc
     1981 cgcgttcgat tgccagattg ccgccgaaat caaagacttt gttcccggcc ccggttttcc
     2041 gtcgcccaag gaactcaaac gcaccgaccg ttttgcacag ttcggcatct gcgccggtta
     2101 ccaggcgctg atggattcgg gtttggactt ggagaaggtc aatcgcgacg aaatcggcgt
     2161 gttcataggc tccggcatcg gtggattgaa gacggttgag gaacagcatt cagtcctgct
     2221 tgaacgcggg gcgaagcgca tttcgccgtt catgatcccg atgctcatcc tgaacatggc
     2281 gtcgggattg ttctcgatgt atttcaaact gcgcggacca agctttgcga cttgttccgc
     2341 ttgcgcgact tccactcacg ccatcggcga ggcgtggcgc accatcaaga tgggagatgc
     2401 gaccgcgatt tttgccggcg gcaccgaggc gacgattgtg ccgatgggca tcggcggttt
     2461 ttgcgccatg cgtgcgatga gcacgcgcaa tgacgacccg caacattcct cgcgtccgtt
     2521 cgacaaggac cgcgacgggt ttgtgatggg cgaaggcgcg ggcgtgctgg tcatggagga
     2581 attggaacac gcgaaagcgc gcggcgcaaa gatttattgc gaaatcgtcg gttacggaaa
     2641 tacttccgac gcgagccaca tcacggcgcc cgcgccggaa ggcgaaggcg cggcccgttg
     2701 catgaagatg gccctgcgca ccggcggcct caatccaacc gatgtctcct acgtgaacgc
     2761 ccacggcact tcgacgccac agggtgatat ttgcgaaacg caggccatca agacggtttt
     2821 tggcgaacac gcccgcaagc tggcggtcag ctcgacgaaa ggcgcgaccg gccacatgtt
     2881 gggcgcggct ggcgcggtgg aaatggctgt ctgcgccaag gccatccaaa ctgatacggt
     2941 ccctcctaca atcaactaca ccacccccga tccggaatgt gatttggatt acgtgccgaa
     3001 cgcggcgcgg caagtgcggg tcaatgccgt cctcaataac tcgttcggat tcggcggcca
     3061 caatgccacc atcgcggcga agaagtttgc ggagtaaaac cgcgaaagaa gactcacgct
     3121 ttaagggcgc agggtcgaag ccccttcttc gtatgagaat ggttccgggc agtacacgac
     3181 tcgttgtgat ctgaattctt ttttacaata aaattcaatc ttctccttga acgatgcgtt
     3241 ggtgaacaag ggctgtaccc agcaattgct taaaaaatcg gagttgtctt taaaatctcc
     3301 ataataatag caaattccga ggtgtttctg agaagaccgg ggacttaatg tccactccag
     3361 catgcaactc tcgttgggca taagttttgc aagcctggca gaacaacctc ccgagttttc
     3421 agacggttcg atatagagca tggaatcgaa gcggtttgtc ctcccgatta tcccactcag
     3481 aaagcacctc tgcataaact cggattccag cgcatgtctt tgtccacaat acaaaaacca
     3541 gattggttgt ttggtcgtat ttcgcaattc cagctttgct cttagcaaat cggatgagta
     3601 gttaagtttg tcataacctt taaaaacgag cgtaggctgg ccaccaaact tttcccacca
     3661 aattgcaata gccagaccaa taatcagcgc catacaactt gcaacgatga tttttttcct
     3721 cttcatccag aaactttaac accctgcttg ggaccaagtt gctccttaaa gtcagagcct
     3781 cctcacgtcg gctcctacga agattgattc aggccacctt caccgcttca ttgacgctgc
     3841 cgcgacggta gccgagcaaa tccagcgtga cgtgcagata accgattttt ttcaaggctg
     3901 tggcgacgcg ttgggcggtg tcgttggcaa caaattttga aagttccgac gggccgactt
     3961 cgatgcgggc catctggcct tgtttcaatt catgatggcg cacgcgcacg tcgtaaaaac
     4021 cgaaatcgcg caggacatat tcggcctgct cgatcatgcg caatttttcc ggtgaaacgg
     4081 cttcgccgta aggaatgcgc gaactgaggc aggccatctg cggcttgtcg gcggtgggca
     4141 atccaagttg cgcggaaaat tcgcggattt cggccttggt caaaccggct tccttcaaag
     4201 gcgcgcgcac ctggaattcc gacgccgcct tcgcgccggg ccgatggtcg ccaacgtcgc
     4261 tggcgttttc gccataggca atcaccgcaa aaccgcctgt gcgcgcgagc ggttcaagct
     4321 cggtgaacag ttcgtgcttg cagaagtagc agcggttgtc gggattggcg aggtattgcg
     4381 gattgctgaa ttcctgcgtc tggacgacct tcacgggaaa accaaatttc tccgcgatgg
     4441 tgagggcttc ttccagttcg cgtcgcggca agcttggcga atcggcgatg gctgcgagcg
     4501 atttttcgcc aaggacttct gatgccatgc gcgcaagaaa aacggaatcc acgccgccgg
     4561 aataggcgac aaggcaggag ccgtaagagc gcagcaacgc gcggagttgg tcaagtttgt
     4621 tcacgagacg atgacaaccc aagttgccat tctcggaggc gaatgtcgag ggcgagaaaa
     4681 ggacggatac gacttatgcg acctataatg gaagttgagg cggaaagtta tttttcgttc
     4741 ttcagcttgt cgacgcgttc tttgaggact tccatcacct tctcgaactc ggtcgcttta
     4801 tgaaaaaaat aatcggcccc ggcatcggaa cagcggcggc gatactccgt ttcatccaag
     4861 ccggtc
//
LOCUS       DASZMM010000219         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3387039, whole genome shotgun sequence.
ACCESSION   DASZMM010000219 DASZMM010000000
VERSION     DASZMM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3387039"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..345
                     /locus_tag="VG754_05215"
     CDS             16..345
                     /locus_tag="VG754_05215"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWF18642.1"
                     /translation="MSALAEKKCAPCDGGTPALKGAELERLAAQLHDDWEVVDEHHLE
                     REYKFKNFRDALTFTNKVGELAEKQNHHPDIYLSWGKVRMTIWTHSIDGLTESDFVLA
                     AKIDELK"
     gene            complement(461..835)
                     /locus_tag="VG754_05220"
     CDS             complement(461..835)
                     /locus_tag="VG754_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWF18643.1"
                     /translation="MPDSLKTAVETLGLESYRRHIFLCADQTEPKCAPKERGLESWEF
                     LKRRLKELNLVGPKPLVYRTKANCLRVCVQGPIAVVYPEGTWYHSCTPEVIERIIQEH
                     LINGKIVEEFAFVKNPMKSEKG"
     gene            complement(845..1882)
                     /locus_tag="VG754_05225"
     CDS             complement(845..1882)
                     /locus_tag="VG754_05225"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWF18644.1"
                     /translation="MAAVPDERLSTIDSLFRDFNRPDAPGASVMVISDGKVLLEKSYG
                     LADLEKKVPVKPDTNFRLASVTKQFTAMAVMQLAEAGKLSLDDPITKFFPEFPEYGKK
                     IIVRNLLNHTSGLLDYEELIPQGTTIPVLDINALHLLMQQDKTYFSAGTQFRYSNTGF
                     AFLALIVERVSGETFAAYLHKHIFAPLHMDQTLAYEAGISTVANRGFGYTQTADGFTR
                     TDQSLTSSVLGDGGVYSSIDDLRKWDEALYGTKLVSRKMLKAAFTPGAATLHDKHVQY
                     GFGWFISDYRGLRNIWHSGNTVGFSTRIERFPDRKFTVIILTNRNDAHLSEIPHQITD
                     LYLFDRETARK"
     gene            complement(2059..2982)
                     /locus_tag="VG754_05230"
     CDS             complement(2059..2982)
                     /locus_tag="VG754_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWF18645.1"
                     /translation="MLAAFLTTIFFSISVICGNRSAQMIGGSEANFWRLTCATGFLAL
                     WAWTFGQGLSGESFPLFLLSGVIGIGIGDVALFQTLPRLGSRLTLMLVQCLTAPFGAA
                     IEWLWLGTQPKPMQMICAAIILVGIGISLAPGKNLHLQKRAILPGIVFSLMAALGNAF
                     GAVLSRKAYEVAHDHGEHNIDGGTAAFQRILGGLLIGGISLLVVKWRTIKGHLMQSGE
                     NGTLPSKEKWRRVLPWVLANSLAGQTLGVSCYQWAFQTTPTYIVLPIVATTPLVAIPI
                     SMAVEKERPSARSLVGGAVAVAGVIGLALLK"
     gene            complement(3032..>3602)
                     /locus_tag="VG754_05235"
     CDS             complement(3032..>3602)
                     /locus_tag="VG754_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18646.1"
                     /translation="LGFEKRERYYAGAEAGWGAQISEQAMVGIVVFADVDLMPDETQI
                     DFSVKRLPTAPKLGTVGLWVGLHGESFLGAGMHHLEARFDYLLLRDQLKEQGVNTMNP
                     FSNFEFLRQAFTEGERWQVSRVRAEKLLSAGLITQEQFEKFMKEGAIGSHLENLQRHG
                     GFKGFNQKSVSVVIAATDPRKSHFQNAPA"
BASE COUNT          852 a         1051 c          936 g          763 t
ORIGIN      
        1 ataggagaac atattatgag cgcactcgct gaaaagaaat gcgcgccttg cgacggcgga
       61 actcccgcct tgaagggcgc cgaactggaa cggctcgccg cacaattgca cgatgattgg
      121 gaagtggtcg atgaacatca cctcgaacgc gaatacaaat ttaaaaattt tcgcgacgct
      181 cttaccttca ccaacaaggt cggcgaactc gccgaaaagc aaaaccatca tccggacatt
      241 tatctgtcct ggggcaaagt gcgcatgacc atttggaccc actcgattga tggactgacg
      301 gaaagcgatt tcgttcttgc cgcaaagatc gacgagttga aatagccgtc cgtattttct
      361 cgaacgcaaa ccaaagcggc agaggattgc cgcagtccaa gacgctgccg cgacattcaa
      421 tccgctctga aaactccgcg aagtcttgcg ttatctgccg ctatcctttt tcgctcttca
      481 tcggattctt cacgaaagcg aattcctcga cgatttttcc gttgatcaaa tgctcctgga
      541 tgatgcgctc gatcacttcc ggcgtgcacg agtgatacca ggttccttcg ggataaacga
      601 cggctatcgg gccctgaacg caaacgcgca ggcaattggc tttcgtccgg taaaccagcg
      661 gcttcggccc gaccagatta agctccttca accggcgttt taaaaattcc catgattcaa
      721 gaccgcgttc cttgggcgcg cactttggct cggtctggtc ggcgcaaaga aaaatgtgcc
      781 ggcgatacga ttccaagccc aaggtttcga ctgcggtttt gagtgaatct ggcatgaagc
      841 aaatttactt acgcgccgtt tccctgtcga aaagatagag gtcggtgatt tgatgcggga
      901 tttccgacag gtgcgcgtcg ttgcggttcg tcaaaatgat gacggtgaac ttccggtccg
      961 gaaagcgttc gatgcgcgtg gaaaaaccga ccgtgttccc gctgtgccag atgttgcgta
     1021 agccgcgata gtcgctgatg aaccagccga aaccatattg aacgtgcttg tcatgcaacg
     1081 tcgccgcacc tggcgtgaag gccgccttga gcatcttgcg cgaaaccaat ttcgtgccgt
     1141 aaagcgcctc gtcccatttg cgcaaatcgt cgatcgaaga ataaacgccg ccatcgccca
     1201 gcaccgaact agtcaggctt tggtccgtgc gggtgaaacc gtccgcagtt tgcgtgtaac
     1261 caaagccacg attcgcaacg gtcgaaatac cagcttcata agccagcgtt tggtccatgt
     1321 gcaacggagc gaaaatatgc ttgtgcagat acgcggcgaa agtttcgccg gaaactcgtt
     1381 ccacaatcag cgccaagaac gcaaaaccgg tgttgctgta ccggaactgc gttccggccg
     1441 aaaaatacgt tttgtcctgc tgcatcaaca aatgcagcgc attgatgtcc agcaccggaa
     1501 tggtcgtccc ttgcggaatc aactcctcat aatccaaaag accggaagta tgattgagca
     1561 gatttcggac aataattttt ttgccgtact ccggaaattc gggaaagaat tttgtgatgg
     1621 gatcgtccaa ggacaatttt ccggcctccg cgagttgcat gacggccatc gcggtgaact
     1681 gcttggtcac ggaagcgagc cgaaaatttg tgtctggctt gacaggaacc ttcttttcca
     1741 agtccgccaa gccatacgat ttttccagca gcacttttcc atcgctgatg accatcacgc
     1801 tcgcgcccgg cgcgtcgggc cgattgaaat cgcggaacaa actatcaata gtgctcaatc
     1861 gttcgtcggg cactgcggcc atggcgcatt ctatggaaaa aatcagcgcg acgacaacaa
     1921 tgaatggaag caggcgactg gaaagtcgcc tgaacccgca gacaagaatg tctgcgctac
     1981 gtggcgcagg ctttccagcc tgcgggttct ggggactttc cagtccccag tcaactgtcg
     2041 atcttaaatg ccggcggttc attttagcaa cgcgagacca atgacccccg caacagcaac
     2101 cgcgccgccc accaacgagc gcgcgctggg ccgttctttc tcaactgcca tcgaaatggg
     2161 aatcgccacc agcggcgtcg tggcgacaat cggcagcaca atgtaagtgg gcgtggtttg
     2221 aaaagcccat tgataacagc tcacgccgag cgtctgcccc gcaaggctgt tggcgagcac
     2281 ccatggcagc acgcgccgcc atttttcctt tgatggcaac gtcccgttct cgcccgattg
     2341 catcaggtgt cccttgatgg tgcgccattt caccaccagc agcgagatgc cgccgatgag
     2401 caatccgccg aggatgcgtt gaaacgccgc tgttccgccg tcaatgttgt gttcgccgtg
     2461 gtcgtgcgcc acttcataag ctttcctgct caataccgcg ccaaatgcat tccccaacgc
     2521 cgccatgaga ctgaaaacaa ttcccggaag aatcgccctt ttctgaagat ggagattttt
     2581 tcccggcgca agcgaaattc cgatgccgac gagaatgatc gccgcgcaaa tcatctgcat
     2641 cggtttgggc tgcgtcccaa gccagagcca ttcgatggcc gcaccgaacg gcgcggtcag
     2701 gcattgcacc agcatcaacg tcagtcgcga accgagccgc ggcaaagtct ggaacaacgc
     2761 cacgtccccg atgccgattc cgatcactcc gctcaaaaga aacagcggga aactctcacc
     2821 cgaaagcccc tgcccgaatg tccacgccca taaagccagg aatccggtcg cgcaggtgag
     2881 ccgccagaaa ttcgcctccg aaccgccgat catctgcgcg gagcggttgc cgcaaatgac
     2941 ggaaatcgaa aaaaagatgg tcgtcaggaa cgcggcaagc atgatttttt ggaaatcgtt
     3001 gccgcaaaca gccggttgtg gcgaagggcg ctcacgccgg agcgttttga aaatggctct
     3061 tgcgcgggtc ggtcgcggcg atgaccacgc tgactgattt ttgattgaag cccttgaacc
     3121 cgccgtggcg ttgtaaattt tccagatggc tgccgatcgc gccttccttc atgaactttt
     3181 cgaattgctc ctgcgtgatg aggccggctg agagcaattt ctccgcgcgc acgcggctca
     3241 cctgccagcg ctcgccttcg gtgaacgcct ggcgcagaaa ttcgaaattg gaaaacgggt
     3301 tcatcgtgtt gacgccttgt tccttcaact ggtcgcgcaa cagaagataa tcaaaccgcg
     3361 cttcgagatg gtgcatcccc gcgccgagaa aactttcgcc atgcaaaccg acccacaaac
     3421 caaccgtccc aagcttcggc gcggtcggga gccgtttcac cgaaaaatca atctgcgtct
     3481 cgtccggcat caggtccacg tccgcgaaca ccacgatacc gaccattgcc tgctcggaaa
     3541 tctgcgcgcc ccaacccgct tccgcaccgg cgtaataacg ctcacgcttt tcaaaaccga
     3601 gc
//
LOCUS       DASZMM010000220         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_370660, whole genome shotgun sequence.
ACCESSION   DASZMM010000220 DASZMM010000000
VERSION     DASZMM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370660"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /gene="mnmA"
                     /locus_tag="VG754_05240"
     CDS             <1..837
                     /gene="mnmA"
                     /locus_tag="VG754_05240"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWF18647.1"
                     /translation="KQVINYFAEEYKAGRTPNPCVMCNEKLKFGTLINRARQLGAEFI
                     ATGHFARVEKSADGTRMLLKRGRDARKDQSYFLFSLRQEQLARTLFPLGELTKTDTRC
                     VARENRLKTADKEESMEICFVPDKDYGRFLQQANLVQKHRGEIVNLHGQVLGHHEGIE
                     FYTIGQRKGLGISSPKPLYVVELDAANNRVVVGDESALERDDFFVERCNWIPFETPPA
                     TMEVSAKIRYNHPGTTATITPEANGRARVKLHVPQRAITPGQACVFYQDDLVVGGGWI
                     GR"
     gene            963..1145
                     /locus_tag="VG754_05245"
     CDS             963..1145
                     /locus_tag="VG754_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18648.1"
                     /translation="MVGLLSAVFTGTNGARLDEPQQHVTIKTRQANSCTLVLPVLLRV
                     TDPRSEAVRGPRPVGF"
     gene            complement(1147..2013)
                     /locus_tag="VG754_05250"
     CDS             complement(1147..2013)
                     /locus_tag="VG754_05250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWF18649.1"
                     /translation="MRPEYRPRSPFGTHMSASILLIIALIVSFGLEYSYRGFTQQYLE
                     LSIDGMRHGYIWQLLTFQFLHDGVVHLASNLLGIWFFGRFIEERLGHAHFWKLYLLSG
                     VAGGLLQTALMFVSPVHFGTSVVGASAGVFGLIAAFAVMEPDATLMAYFFIPLRAKYL
                     LYIELGVALYFIIGPTSHVAHAAHLGGILFAIAYIRWGLDLSRNWSDWNPLQRKMRRE
                     RMIKAATIKPSLASLRRRPRPDVPTDLPSDEFISKEVDPILDKISAHGIQSLTDRERA
                     ILQAARAKMSKR"
     gene            complement(2047..>2677)
                     /locus_tag="VG754_05255"
     CDS             complement(2047..>2677)
                     /locus_tag="VG754_05255"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWF18650.1"
                     /translation="FSIDKYFALSVEGLRHGYIWQLLTYQFMHAVPEPWHLLLNSWAI
                     FVFGRIVEMTLGKKRMLWLYFLSGTLGGLVQMTGMMLLPGLFGTGAAVGASAGAFGLV
                     AAFAMLYPRERLYVLLFFILPLRMRATTFLWVAVGLSLFGIFEPLFANSLPSFLKIDE
                     IFGKVAHAAHLGGIVAGALLARQWARVRVPPRIIEVEAKTSLKTTPAPD"
BASE COUNT          656 a          724 c          782 g          515 t
ORIGIN      
        1 aagcaggtca tcaactattt tgcggaggag tataaggcgg gtcggacgcc gaatccgtgc
       61 gtgatgtgca atgagaagct gaagttcggc acgttgatca accgggcgcg gcagcttggg
      121 gcggaattta tcgcaacggg acattttgcg cgggtggaaa aaagcgcgga tggcacgcgg
      181 atgctgctga agcggggtcg tgatgcgcgc aaggaccaga gttattttct tttctcgctg
      241 cgccaggaac aactggcgcg cacgctcttt ccccttggcg aactgaccaa gaccgacacg
      301 cgctgcgtcg cccgcgaaaa ccggctcaag accgccgaca aggaggagag catggaaatc
      361 tgtttcgtgc cggacaagga ttacggacgt tttttgcaac aggcaaattt ggtgcagaag
      421 catcgcgggg aaatcgtgaa tcttcacggc caggtgctgg gtcatcatga aggcatcgag
      481 ttttatacga ttggccagcg aaaaggcttg ggcatttcat cgccaaaacc gctttacgtg
      541 gttgaactgg atgccgcgaa caatcgtgtc gttgtcgggg acgaatcggc attggaacgg
      601 gacgattttt ttgtcgagcg ttgcaattgg atcccgtttg aaacgccgcc tgcgacgatg
      661 gaagtgagcg caaaaatccg ttataaccat ccgggcacca cggcaacgat cacccccgaa
      721 gccaatggcc gcgcgcgggt caaactgcat gttccgcaac gcgccatcac gccgggccag
      781 gcatgtgtct tttatcagga tgatttggtt gtcggcggcg gctggattgg gcggtgagaa
      841 cgtgcgaaat cggaggagat taggaaatca ggaactcacg aagaggaact gcgggtgcca
      901 gcttaatccc cgttaggcgc accgcgaaat gcccccgatt tgttggccat gccggggcgg
      961 agttggtggg gctattgtcc gccgtgttca ccgggacgaa cggagcgcgg ctcgatgagc
     1021 cgcagcaaca tgtgaccatc aagacacgcc aagccaattc atgcacgctc gttcttccgg
     1081 tgttgctgcg ggtcaccgac ccgcgctccg aggcagtgcg tggaccccgc cccgttggtt
     1141 tttgaatcac cgcttcgaca tctttgcgcg ggcggcctgg aggatggcgc gttcccggtc
     1201 ggtcaggctt tggatgccgt gggcggaaat tttgtcgaga atgggatcga cttccttgct
     1261 gatgaattcg tcggacggca gatccgtcgg cacatcgggg cgcgggcggc ggcggagact
     1321 ggcgagagat ggcttgatgg ttgcggcttt gatcatccgt tcgcggcgca ttttgcgttg
     1381 aagcggattc caatccgacc agtttcgaga gagatccaga ccccagcgga tataggcgat
     1441 ggcgaaaaga atgccgccca aatgcgcggc gtgggcaaca tggctcgtgg gaccgatgat
     1501 aaaatacaat gcgacaccaa gttctatgta gagcaggtat ttcgcgcgta acggaatgaa
     1561 gaaatacgcc attaaggtcg cgtccggttc catcacggcg aaagcggcta tcaatccaaa
     1621 cacccccgcg gaagcgccga cgacgctcgt gccgaaatgc acgggcgaaa cgaacatgag
     1681 cgcggtttgc aacaaacccc ctgccacgcc actcagcaag taaagtttcc aaaaatgagc
     1741 gtgaccaaga cgttcctcga tgaaccggcc aaaaaaccag atgccgagca gatttgaggc
     1801 gagatggaca acgccatcat gcagaaattg aaacgtgagc agttgccaga tgtaaccgtg
     1861 ccgcatccca tcgatgctca attccagata ttgctgggta aagccccgat aggaatattc
     1921 cagcccgaac gagacaatca gcgcgatgat aagcagaatg ctcgccgaca tgtgcgtccc
     1981 gaaaggggag cgggggcggt attcagggcg catataatgt ctgtcatcca gcatacctga
     2041 ttaaacctag tccggcgccg gggtggtttt caatgaagtt ttggcttcaa cctcaataat
     2101 tctcggcgga acgcgcaccc gcgcccattg acgcgccagc aacgctcccg caacgatgcc
     2161 tcccaagtgc gccgcgtggg cgaccttgcc gaaaatttcg tcaattttca gaaatgaggg
     2221 cagagaattt gcaaacagcg gttcaaaaat gccgaacagc gacaatccca cggccaccca
     2281 aagaaatgtc gtggcgcgca tccgcagcgg aaggatgaaa aacaaaagca cgtaaagccg
     2341 ttcgcgcgga taaagcatcg caaatgccgc caccaaccca aacgcgcccg ccgaagcgcc
     2401 gactgccgcg cccgtcccga acaatcccgg cagcagcatc attcccgtca tttgcaccaa
     2461 tccgccgaga gttccgctca ggaaataaag ccagagcatc cgttttttgc ccaaggtcat
     2521 ttcaacaatc cttccgaaaa cgaaaatcgc ccaggaattg agcagcaaat gccacggttc
     2581 cggaaccgcg tgcatgaatt gataggtcag cagttgccag atgtaaccgt gccgcaatcc
     2641 ctcgacgctc aatgcgaagt atttatcgat cgaaaat
//
LOCUS       DASZMM010000221         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2562989, whole genome shotgun sequence.
ACCESSION   DASZMM010000221 DASZMM010000000
VERSION     DASZMM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2562989"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VG754_05260"
     CDS             complement(<1..234)
                     /locus_tag="VG754_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18651.1"
                     /translation="MRNIIVIVFLAAVILVGAIWYYSGKNQHADQARQSLTSAASQTK
                     EFVQDKFGTSNLSADNIKDEMARTGQVIREKAQQ"
     gene            complement(348..1061)
                     /locus_tag="VG754_05265"
     CDS             complement(348..1061)
                     /locus_tag="VG754_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HWF18652.1"
                     /translation="MKYIDLTLPTPAENLACDEALLEACEAGLADEILRFWEPQEPFV
                     VVGYANQIAREVNLEACKGRNIPGLRRCSGGGTVLQGAGCLNYSLLLKIPDAGPLRSI
                     SGTNSFIMERHRAALQSLLKGRVHVQGHTDLTLDNLKFSGNAQRRRKNFLIFHGTFLL
                     RFDLPLVEQFLRMPSKQPEYRQSRPHTEFLTNLNISPEVVKSALREIWNANTPLPALP
                     APPRELLDKYLTDEWNFKF"
     gene            1289..3922
                     /gene="aceE"
                     /locus_tag="VG754_05270"
     CDS             1289..3922
                     /gene="aceE"
                     /locus_tag="VG754_05270"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWF18653.1"
                     /translation="MQEWFELLEPAVEFVLKSQEPTRATRFFDRLVERLRQSGVKVPQ
                     VVSTPYINTIPLEKQPAYPGDWQTEVRIKSFIRWNAMAMVVNANAVKEGLGGHISTFA
                     SSATLYEVAFNHFFRGRHGDFPGDLIYFQGHASPGIYARAFMEGRLTEKHLQNFRQEL
                     SEGGGLSSYPHPYLMPDFWQFPTVSMGLGPIMSIYQARFNRYLRARGIIKGEEPKVWC
                     FIGDGESDEPETLGSLTLGARENLDNLVWVINCNLQRLDGPVRGNGKIIQELEALFNG
                     AGWNVIKVIWGSDWDELLARDRSGLLLKRMGEVVDGDYQKYSVEPGSYTRKHFFGKYP
                     ELLELVNHLTDEQIRKLLRGGHDTRKVYAAYAAAMAHKGSPTVILAKTIKGYGLGEAG
                     EGKNITHQQKKLNEKELREFRTRFGIPLNDEEVVETPFYRPDPESKEIKYLLERRKKL
                     GGFLPARVVEAPALETPKLEAFSELLKGGRANSTTEVFGRMLAMLLRDRNVGKQIVPI
                     IPDEARTFGLDALFKDIGIYAPKGQLYEPVDKESLLYYNETKNGQILEEGITEAGSMS
                     SFIAAGTSYATHGVNMIPFYIYYSMFGFQRIGDLMWLAGDIKAKGFLLGATYGRTTLN
                     GEGLQHQDGHSLLTASSIPTLLTYDPAFGFEVATIIADGLHRMYEKGEDLFYYLTLYN
                     EKYLMPQMPDDCREGILKGLYKFKPSLEKSKLRAQIFGSGPIINSALRAQTILAEKYG
                     VAADVWSATNYKWLRNDALAARRWNMLHPSETPRKSYIETLLANEQGPFIAVSDNMKM
                     VPDQIAPWVPGGLMTLGTDGFGRSDTRPSLRRFFEVDAELTVIATLYALAERGVLEKK
                     IVGQAIKDLNVDPEKAHAVIL"
     gene            4072..>4238
                     /locus_tag="VG754_05275"
     CDS             4072..>4238
                     /locus_tag="VG754_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWF18654.1"
                     /translation="MDVKLPNLGEGADTGTVVNVFVKEGDKVEKDQPLLELENEKAVA
                     TIPSTAAGVVS"
BASE COUNT          981 a         1121 c         1205 g          931 t
ORIGIN      
        1 ttgctgggct ttctcgcgga tgacctggcc ggtgcgggcc atctcatcct ttatattgtc
       61 cgccgagaga ttcgacgtgc cgaacttgtc ctggacaaat tccttggttt gcgaagccgc
      121 gctggtcaaa ctttgccgag cctggtccgc gtgctgattt ttgccgctgt aataccagat
      181 ggccccaaca aggatgaccg ctgccagaaa aacgatgaca atgatatttc tcatgcgttt
      241 agaactggat aaatcttcat ttgtaatggg caaaagtcaa atggggcagg ttcctgaaaa
      301 ctgcggcgaa tcggacgaat gcgacctatg ggataaaatc ccaatgctca aaatttaaaa
      361 ttccactcgt cggtcaaata cttgtcgagc aattcccgcg gcggggccgg caaagcgggc
      421 aggggagtgt tggcgttcca aatttcgcgc aaagcggatt taacaacttc cggcgagatg
      481 ttcagattcg tcaggaattc agtgtgtggg cggctttgac ggtattcggg ttgcttcgat
      541 ggcattcgca ggaattgttc gacaagcggg aggtcgaaac gcaacaaaaa cgtgccgtga
      601 aagatgagga aatttttacg gcggcgttgc gcgttgccag agaatttcag attgtccagg
      661 gtcaagtcgg tgtggccctg gacgtggacg cgccccttta gcaaggactg caacgcggcc
      721 cgatgccgtt ccatgatgaa cgagttcgtt cccgaaatgc tgcgcaaagg cccggcatcc
      781 ggaattttca acaggagcga ataattgagg caacctgcgc cttgcaaaac cgtccctccg
      841 ccgctgcatc ggcgcaaacc cggaatgttc cgtcctttac acgcttcgag attgacctcc
      901 cttgcaatct ggttcgcgta accgactaca acgaacggct cttgcggctc ccagaaccgg
      961 aggatttcgt cggccaaacc cgcttcgcac gcctccaaaa gcgcctcgtc gcaagccagg
     1021 ttctcggcgg gggttggcag ggttaagtca atatatttca ttgtgttcgg actgtgctaa
     1081 tatattgttt ccgggccgtc acggatagcg tcttgtctaa tccgccagaa ttgagttaga
     1141 cttatgcgta tctcggtgta ttcggccacg gtgattcaat caatggcagc aggataagaa
     1201 cgtgaaattg cagacagaac aacctaaaat ctcggcgcgc gacggagggc atgaagagcc
     1261 ttccgtgagg aagaacaacg gcacggaaat gcaggaatgg ttcgagttgc tcgaacccgc
     1321 cgtggaattt gtcctgaaaa gccaggaacc gacccgcgcc acgcggttct ttgaccgatt
     1381 ggtggaacgt ctccgccaat ccggcgtcaa ggtgccgcag gttgtcagca cgccctacat
     1441 caacaccatc ccgctggaaa aacaacccgc ttatccgggc gattggcaga ccgaagtccg
     1501 catcaaaagt tttatccgct ggaacgcgat ggcgatggtc gtcaatgcga acgccgtcaa
     1561 ggaaggcctt ggcgggcaca tctcaacttt cgcttcctcg gcgacgctct atgaagtcgc
     1621 tttcaaccat ttctttcgcg gcaggcacgg cgattttccc ggcgacctca tttattttca
     1681 gggccacgcc tcgccgggga tttatgcgcg cgccttcatg gaaggccggc tgaccgagaa
     1741 gcatttgcaa aatttccggc aggaactctc ggaaggcggc ggactttcct cctatccgca
     1801 tccttatctg atgccggatt tctggcagtt cccgaccgtc tcgatgggcc tcggccccat
     1861 catgtccatc tatcaggcgc ggttcaatcg ttacctgcgc gcgcggggca tcatcaaggg
     1921 cgaggaaccg aaagtctggt gtttcatcgg cgacggtgaa tccgacgaac cggaaacgct
     1981 cggttctctc acgcttggcg cccgcgaaaa tttggacaat ctcgtatggg tcatcaattg
     2041 caacctgcaa cgtctggacg gcccggtgcg cggcaacggc aagatcatcc aggaactcga
     2101 agcgctcttc aacggcgcgg gctggaatgt catcaaggtc atttggggtt ccgattggga
     2161 tgaattgctg gcgcgcgatc gttccgggct tttgctcaag cgcatgggtg aagtggtgga
     2221 cggcgattat cagaaatatt ccgtcgagcc cggcagctac acgcgcaaac atttcttcgg
     2281 caaatatcct gagttgctgg agttggtgaa tcatttgacg gacgaacaga tccgcaagct
     2341 gttgcgcggc gggcatgaca cgcgcaaagt ttatgcggct tatgctgccg cgatggcgca
     2401 caaaggttcg ccaacggtca tcctcgcaaa gaccatcaag ggctatggct tgggcgaagc
     2461 cggcgaaggc aagaacatca cgcatcaaca gaaaaagctg aacgaaaaag aactgcgcga
     2521 gttccgcacc cggtttggca ttccgttgaa cgacgaggaa gtcgtcgaga cgccgtttta
     2581 tcgtcctgac ccggagagca aggaaatcaa atatcttttg gaacgccgga agaaactcgg
     2641 cggttttttg cctgcgcgcg ttgtcgaagc gccggcgttg gaaactccca agctggaggc
     2701 tttctcggaa ttgttgaaag gcggccgggc caattcgacg acggaagttt ttggaagaat
     2761 gctggcgatg cttctgcgcg accggaatgt cggcaagcag attgttccca ttattcccga
     2821 cgaggcgcgc acgttcgggc ttgatgcgct tttcaaggac atcggcatct atgcgcccaa
     2881 gggacagctt tacgagccgg tggacaagga atcgttgctc tactacaacg aaaccaagaa
     2941 cgggcaaatc ctggaggaag gcatcactga ggctggttcg atgtcatcgt tcatcgcggc
     3001 gggcacttcg tatgccacgc atggcgtgaa catgattccg ttctacatct attattcgat
     3061 gttcggtttt cagcgcatcg gcgatttgat gtggctcgct ggagacatca aggcgaaagg
     3121 ctttttgctc ggcgcgactt acggccgcac gacgctgaac ggcgaaggac ttcaacatca
     3181 ggacggccac agtctgctca cggcgagttc gatcccgacg ctgctgacct acgatccggc
     3241 gttcgggttc gaggtcgcga ccatcatcgc ggacgggttg caccgcatgt atgaaaaagg
     3301 cgaggacctt ttttattacc tgacccttta caatgaaaag tatctgatgc cgcagatgcc
     3361 ggatgactgc cgcgaaggca ttttgaaggg cttgtataaa ttcaagccgt cgttggaaaa
     3421 atcgaaattg cgcgcgcaga tttttggcag cgggccgatc atcaattcgg cgttgcgcgc
     3481 gcaaaccatc ctcgcggaaa aatacggcgt ggccgccgat gtctggagcg cgaccaatta
     3541 caaatggctg cgcaacgacg cccttgccgc gcggcgctgg aacatgctgc atccgtccga
     3601 aacgcccagg aagtcttaca tcgaaacttt gctggcgaat gagcaggggc cgttcatcgc
     3661 ggtttccgac aacatgaaga tggtcccgga ccagatcgcg ccatgggtgc ccggcggctt
     3721 gatgactttg gggacggatg gttttggacg cagcgacacg cgcccgagcc tgcgccggtt
     3781 tttcgaagtg gacgcggaat tgaccgtcat cgccaccctc tacgcgctcg ccgaaagggg
     3841 cgtcctcgaa aagaagattg tcggacaggc gatcaaagac ttaaatgtgg acccggaaaa
     3901 ggcgcacgcg gtgattctgt gagggacgat gatggttaca tggttgcaag gttacatcgt
     3961 tacaagccat agctcgcacc atgcaacttt ttcccccatg taaccatgta acgttttaac
     4021 gatgtaacca ttcaacgcct tgaccgattt atcagtttaa tcagaataat tatggacgta
     4081 aaactgccca accttggcga aggggccgat accggcaccg tggtgaatgt atttgtcaaa
     4141 gaaggcgaca aggtcgaaaa agaccagccg ttgctggaac tcgaaaacga aaaggccgtc
     4201 gccacgattc cgtcaactgc cgccggggtc gtttccaa
//
LOCUS       DASZMM010000222         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1472048, whole genome shotgun sequence.
ACCESSION   DASZMM010000222 DASZMM010000000
VERSION     DASZMM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472048"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VG754_05280"
     CDS             <1..376
                     /locus_tag="VG754_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HWF18655.1"
                     /translation="NAHRKPVNGSCVLVVGLAYKPNVDDDRESPSYVLMDLLGERGAD
                     VSYYDPYVSVIPKTREHPHWAGTRSVGWDKETISSFDAVIIATHHQAINYQELSDWTE
                     CIIDTRNAMAGIKTKPGQVWKA"
     gene            451..1746
                     /locus_tag="VG754_05285"
     CDS             451..1746
                     /locus_tag="VG754_05285"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWF18656.1"
                     /translation="MQPSLLQGRLPIVGARKRNLKSTKSGSASAAMLCLFGAAAVAKG
                     QSVVMPPPSYSVTPPAVQSELGGQIGEGTNPALPDFANLLQWGPVTIHPHASYQLLYA
                     DGLQSAPGQQQSSVVQTLSPGVFLNIGTHWTLDYSPTITFYSSSQLHDTVNQTMSLSW
                     GTFYEDWSWGFSQGLNIADNPLVQTATQTSTESYNTSLTASHRFNSVMSLDMSLSQSI
                     NSADQFSSSKSWNTMEYLNYQLFPRMDAALGVGFGYDAVSLGPDMSHETVQARLSWSV
                     ANKTGLVVHGGVEDLQYLGGGVPETITPVFGVSLQYQAFSHTSISLNADRSISPALFA
                     SQAAQTTTLGVSLGQRFLQHFNFSVSGSYGLTDYLSSSAGVAAGRSDSYYSISSQLSW
                     AFLKRATVSLTDQYSVNNSNANGFGFSSTQVGIMVGYGF"
     gene            1863..2801
                     /gene="xrtC"
                     /locus_tag="VG754_05290"
     CDS             1863..2801
                     /gene="xrtC"
                     /locus_tag="VG754_05290"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPDSG-CTERM-specific exosortase XrtC"
                     /protein_id="HWF18657.1"
                     /translation="MESKITGVMMVQPTSAMKFKASSQWKAFLLATCILTICFSVPLF
                     HLARFAWQNELYSYILLVPFISWYLARSKKTGTTADSEPLRGAGVVCMGAGAMMLAWY
                     FEARFSGAMRHYEDGLAMLALAFFFFFIGLGLALLGRETMRSMAFPFGVMFFIVPMPA
                     FVRDGIEKFLQYGSAYAAHGLFTLSTTPFVRDDLTFILPGMPQGISVAPECSGIHSTL
                     ILFITSLLAGYLFLHSPWNRAILTLAVVPLGLLRNGFRVFTLGQLCVHYGPQMLDTPI
                     HHKGGPIFFALSLIPFFALLAFLYKRERRSANPVKN"
     gene            2809..>3080
                     /locus_tag="VG754_05295"
     CDS             2809..>3080
                     /locus_tag="VG754_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18658.1"
                     /translation="MRNLNLLLTSLALLAITGPGCTKHAKMARHMEHADKYFADGDYD
                     KAEIEYINVLQLDGKSARAYGRIGIMSLEQGRMARAYTPLQRACAL"
BASE COUNT          670 a          819 c          832 g          759 t
ORIGIN      
        1 gaacgcgcat cgcaaacccg tgaacggcag ttgcgttctc gtggttggtc tcgcttacaa
       61 accgaacgtg gacgacgatc gtgagtcgcc cagttatgtg ctgatggatt tgcttggcga
      121 acgcggcgcg gatgtttcct attacgatcc ttatgtcagt gtgattccca aaacccgcga
      181 gcatccgcat tgggcgggga cgcgttcggt cgggtgggac aaggaaacga tttcaagttt
      241 tgacgccgtc atcatcgcga cgcaccacca ggcgatcaat tatcaggaat tgtcagactg
      301 gacggagtgc atcattgaca ctcgaaatgc catggcagga atcaagacaa aacccggcca
      361 ggtttggaaa gcctgattcc ctcaaatacg cggcaagacg cccagaactg acaaccaact
      421 cagcgcaggg actgacggaa aagcgccatc atgcaaccat cattacttca agggcgcctg
      481 cccattgtgg gcgcgcgcaa acgaaacctc aaatcaacaa aaagtggcag cgcaagcgcg
      541 gccatgcttt gcctttttgg cgcggcagcg gtcgcaaagg gacaaagtgt cgtgatgccg
      601 ccgccctctt attccgtgac gccgccggct gtgcaatcag aactcggcgg gcaaattggc
      661 gaaggaacaa atccggcatt gccagatttc gcgaacctcc ttcagtgggg tccggtcaca
      721 atccatccgc atgcttccta tcagcttttg tatgctgatg ggctgcaatc cgcgccgggg
      781 cagcagcaat cgtcggtggt ccaaacgctc tccccagggg tgtttctgaa tatcgggacg
      841 cattggacgc tggactacag ccctaccatc actttttact cgagcagcca gcttcatgac
      901 actgtcaatc agacgatgag cttgtcttgg ggaacctttt atgaggattg gagttggggg
      961 ttttcgcagg gcctcaacat cgctgacaat ccgctcgtac aaaccgccac tcagacttcc
     1021 actgagtcat acaacacatc gctcaccgca tcgcaccgct tcaacagcgt gatgtcgttg
     1081 gacatgtccc tgtcgcagtc cattaactcg gcggaccaat tcagcagttc caaatcatgg
     1141 aacacgatgg aatatctgaa ctatcaactt tttccccgaa tggatgcggc ccttggggtc
     1201 ggatttggat acgacgccgt gagcctgggg ccggacatgt cgcatgaaac tgtgcaggcg
     1261 cgtttgagtt ggagcgtggc gaacaaaacg ggcctggtgg tgcatggcgg tgtggaggac
     1321 ctgcaatatc ttggcggcgg cgttcccgag accatcaccc cggtttttgg tgtgtcgctc
     1381 caataccagg ctttcagcca cacctcaatt tccttgaacg cggaccgttc aatcagtccc
     1441 gcgctgttcg ccagccaggc tgcgcaaacc accacgttgg gcgtgagtct tggccagcgc
     1501 tttttgcaac attttaattt cagtgtttcc ggttcgtacg gcctgacgga ctatctatct
     1561 tcgtctgcgg gcgtagcagc gggacgatcc gacagctatt attccatttc ctcccaacta
     1621 agctgggcat tcctgaagcg cgcgacagtt tcgctgactg atcaatacag cgtgaacaat
     1681 tccaatgcca acggcttcgg tttctcaagc acccaggttg ggatcatggt gggatacgga
     1741 ttttaaagtc atatcaaatt ccatacacgt ttggcctctt atcaagagca aaaaaatgcc
     1801 gttcgctgat tgttgcctga attcttttgc ccaatccgat gaccactgaa aaatccatca
     1861 acatggaatc gaaaattacc ggagtgatga tggttcaacc aacttccgcg atgaagttca
     1921 aagcgtcatc ccaatggaag gcgtttttgc tggcgacttg cattctgacg atctgcttca
     1981 gtgtgccttt gtttcatctg gcgcggtttg catggcaaaa tgaattgtac tcctacatcc
     2041 ttctcgtgcc tttcataagc tggtatttgg cacgttcgaa aaagactgga acgacggcgg
     2101 attctgagcc gttgcggggc gcgggcgtgg tgtgcatggg agcaggcgcc atgatgttgg
     2161 cgtggtattt tgaggcgcgc ttttccggcg cgatgcggca ctacgaggat ggtctcgcga
     2221 tgctggcgct ggcatttttc ttttttttca tcggtttggg tctggcgctc cttggaaggg
     2281 aaacaatgcg ctcaatggca tttccatttg gcgtgatgtt tttcatcgtg ccaatgccgg
     2341 cgttcgtaag ggatggaatc gagaaatttc tgcaatacgg ctccgcgtac gccgcgcatg
     2401 ggttgttcac gttgtcaacg accccctttg tccgcgacga tttgactttc atattgcctg
     2461 gcatgccgca aggaatcagc gtcgcgccgg agtgcagcgg aatccattca acgttgatcc
     2521 tgttcatcac cagtttgctt gccggatatt tgtttctgca ttcgccatgg aaccgggcga
     2581 ttttaacatt ggctgtggtt ccattgggcc ttttgcggaa cggtttcagg gtgtttacac
     2641 tcgggcagct ttgtgtgcat tatgggccac aaatgcttga caccccgatc caccacaaag
     2701 gtggcccgat cttttttgct ttgtcgttga ttcccttctt tgcgttgctg gctttccttt
     2761 acaaacgtga acggcggtcc gcaaatccgg tcaaaaattg aatcaattat gcgaaattta
     2821 aatctgcttc tgacgtcgct cgccctgctg gcaatcaccg gtcctggctg caccaaacac
     2881 gcgaagatgg cgcggcacat ggagcacgcc gacaaatatt tcgcggacgg tgattacgac
     2941 aaggcggaga ttgaatacat caacgtgctg caacttgacg ggaaatcggc gcgcgcatac
     3001 gggcgcatcg gcatcatgtc cttggagcag ggaaggatgg cccgtgctta cactccattg
     3061 cagcgggcgt gcgcgctcga
//
LOCUS       DASZMM010000223         6233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1736676, whole genome shotgun sequence.
ACCESSION   DASZMM010000223 DASZMM010000000
VERSION     DASZMM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736676"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..456
                     /locus_tag="VG754_05300"
     CDS             85..456
                     /locus_tag="VG754_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18659.1"
                     /translation="MDRPSEMVAICDVSGSNDPSQINGDADAAWLDTVWAGNSGPSQS
                     VTGDNGRVQTAHARHNNKINIIYVDGHSAPSLPSQIVWGQFWGVFQPGVTLKTSGTSQ
                     QSDAAISSTKLDPQEWSSLQE"
     gene            973..2403
                     /locus_tag="VG754_05305"
     CDS             973..2403
                     /locus_tag="VG754_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18660.1"
                     /translation="MKNNIFAISIVLLALACSPSSRAASVTASGSGNWDSTSPDAPWP
                     GGTVPQSADDVIVNAPNTITVVNPAAANSLEVDGSGVVQNNSALTISGNIFGTGTLAQ
                     GVNSTLNVGGGTATYANGSISNLLTSAAGNTVNYTGNAFFAKRQGYFNLTFSGFGSFY
                     TANVGIPGDLASGPTPIAGNLILSGPSVQLVNDMPISGNLTIGAGSQFDPSCNAFSVG
                     GETIVNGTLTDGCGISGLTDVLNDLTINPGGTLSMGDVTNWSVSGSITNNGSMQGINH
                     ASITLVGSGRLAGSSGVTVPQLNLLGTYEVDAPVTVTTNIVLKGTLIMNVNNSVATPN
                     QLIYRNGTLFYGGNLLVSNVGPALVSGNSFQLFNAASYGGAFSETLPSLSPGLIWQDD
                     SLVNGTFVVTGTSTGGTGSPTLSYSVSGNQMTLSWDAATYPGYVLQSSAFLGATASWS
                     DVAGGNIAPVPITMSGANTFYRLHHP"
     gene            2544..4313
                     /locus_tag="VG754_05310"
     CDS             2544..4313
                     /locus_tag="VG754_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18661.1"
                     /translation="MPAVKAATFTAAATGNWSSGATWGNSGNNVVGSGVPGASDSAVI
                     VSGKTVTYDSSAPAIAGTVEVAGSMVVSKSGGTFGDFTIDSGGTFSFGGQVPVTFSGN
                     FTNNGNMNISSQGQTALTTYSGIGNVIAGNVTNQIANVTGTYTNIGMLVTGVNATGTL
                     NLEGSGTLVNLGTILYNCNKAPTVTTLDCSAPGNLFIWAGVNMSSTTPKATAYYNLII
                     GNTGSAGFPLAGAGLSIANNLTVTNSGNITSWPANGAIGGTLSYFTTSTTAAASFPTA
                     FSVGGFVQTNGVLAMAANDVLTVTGTGAGTWTRTGGTFNQNSSSMVKFTGAAPVIGGT
                     VTNNFANLYIDSTAANAIASTNLNVTNTLTIVAGASLDVTAFNGGAMTMLNAESLLNG
                     GMLVGNVTAPSSAKIFAGLDGSYGTNIISGNLTMSSTNSSINLDVNSSATAPNDLLMV
                     GGALSLNNTPIKLKAPSAGAAIDTSADYTLVTAGSITGVPLLTWITAPANATNYSLVT
                     SSTTMALHYVGAVISNPPTLASSVNGNQMTFSWNTATYPGFFLQTSPTLAPGSWTPVP
                     AGDTSPVLITIDPSTNAFFRLAQ"
     gene            4324..5550
                     /locus_tag="VG754_05315"
     CDS             4324..5550
                     /locus_tag="VG754_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18662.1"
                     /translation="MKPFLALFFAVALSTLGRAENATVVESFENGIDNVSIVTNAGGR
                     GALSPPGVSLSLHVKAGEGDASLTHGNKSLKVELSGKEKFGADFRINFSAEASEQIRK
                     AVASPDIARYILRYDVIFPSMDDFAYFNSILYLGDCRDALIGASGKRSMSVALDLLTG
                     LPESGPITLVMADDFAYRADSKLASSNVTLYIDNIRIVDTYAPGCKPVVYVLQSFEDT
                     NDPIGGITNFSDWDNGTNTTRTRFKSYAASGPDDLRVTEGKNALEVENNAPLPWHADF
                     TLPFNNTKLAEVLKLDAPTEQQPTAEELSHYTLRWDITYPDMTNEWMNSTFHTMETFM
                     PVIQVRHEKPTNQRLTYSLTLDQVRWGKRNDIYPVLTFITEGPQKTHNIKIYYDNFRL
                     IETHKSPKSDVAGREK"
     gene            complement(5613..>6233)
                     /locus_tag="VG754_05320"
     CDS             complement(5613..>6233)
                     /locus_tag="VG754_05320"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HWF18663.1"
                     /translation="KLAAKDGCAAAFTSAFGTPEITPEKIGLALEQYLLSLTSFDSKF
                     DRALRGQEQLTAEEQRGFQLFSTEYDPRRGQFGADCFHCHGGALFQSQTFANNGLDAE
                     FSDTGRYAVTHRDGDQGKFAVPSLRNIALTAPYMHDGRFRTLEEAVEHYCTGMKRSAT
                     LDPNLAKHPDGGVPLKKADKQALVAFLKTLTDERFQQEKVQSHYTL"
BASE COUNT         1431 a         1684 c         1672 g         1446 t
ORIGIN      
        1 ctttaacgag atcgggattt tcgggggacc tgatcccgcc acgggcttca tggtcgggaa
       61 tattcaaaaa ttcaaatcaa cagtgtggac agaccgtcgg aaatggtggc catctgcgac
      121 gtgagcggct cgaatgatcc ctcgcaaatc aacggggatg cggacgcggc gtggctggac
      181 acggtgtggg ccgggaattc cggcccgagc cagtccgtga ctggagataa cgggcgcgtc
      241 cagactgcgc atgccagaca taacaataaa atcaatatca tttatgtgga tggccattca
      301 gcgccaagtc ttccgagcca gattgtatgg ggccagtttt ggggtgtctt tcagcccggg
      361 gtgacgctta aaacatccgg cacgagccag cagtcagacg cggccatcag cagcacgaag
      421 ttggatccgc aggagtggtc ttcgttgcag gaatgagatg cgcaatgggt tggattgggc
      481 agtttgcaaa accggtttgg cgtgcgttcg tcgtgtgcgt caaccgccgg cttgagcgga
      541 tggcgcccgc gggttacgaa gatgagactg ggttccatga gggcagttcg gaacctgatg
      601 ggcatagttc tttttccata atcggcggcc aaagtatcac caaactagac tcgcgaggat
      661 agttaaattt agaagattaa tcacacctag ccatactctc tcgcattgaa tcttgagtgc
      721 tgaggcaaaa acctgttgtt ttttacaacc gttcgaaagt cactttaaag cggtgatgga
      781 tatttggccg ccgttaaatg cttcattgac tttcaatctt gtaactcccc ttgtccccga
      841 ttcatgcgac tacaaaacgt tttccaatcc agctaaggtt gcgatgaagc ccttaaaaaa
      901 ccgaacggac tcgatgcttt tattcaattt gatggaaata gcgccataac tcagcctgaa
      961 ctgactaact acatgaaaaa taatatattt gccatctcaa ttgttctgct cgcgctggct
     1021 tgctccccct caagccgcgc tgccagcgtg acggccagcg gcagcggtaa ttgggattcg
     1081 acttcacccg acgctccgtg gcccggtggc accgtgccgc aaagcgccga tgatgtgatc
     1141 gtcaatgccc ccaataccat tactgttgtc aaccctgccg cggccaacag cctggaagtt
     1201 gacggaagcg gcgtggtcca gaataacagt gcgctgacga tttctggtaa cattttcggc
     1261 accggcacct tggcgcaagg tgtgaattca accttgaacg tagggggtgg aaccgccact
     1321 tatgccaatg gatccatttc caacctcttg acctccgcgg cgggcaacac tgtgaattac
     1381 accgggaatg cctttttcgc caaacggcaa ggttatttca atctcacctt tagcgggttc
     1441 ggcagtttct acaccgccaa tgttgggatt cctggcgacc tggcctccgg cccgactccc
     1501 atcgccggca acttgatcct gagcggtccc agcgtgcaac tcgtcaatga tatgcccatc
     1561 tccggcaacc tgacgatcgg cgcaggttcg cagtttgacc cttcctgcaa tgcgttttct
     1621 gtcggcggcg aaacgatcgt gaacggcacg ttgaccgacg gttgcggcat ctccggcctg
     1681 accgacgttt taaacgatct gaccattaat cccggcggca ctttgagcat gggggacgtc
     1741 accaactggt ccgtaagcgg cagcatcacc aacaatggct cgatgcaagg catcaatcac
     1801 gccagcatca ctttggtcgg ttccgggcgg ctggcaggtt ccagcggtgt gaccgtgccg
     1861 cagttgaatt tgttgggaac gtacgaagtg gacgcgccgg tgacggtaac aacgaatatt
     1921 gtcttgaaag ggacgttgat catgaacgta aacaattcgg tggcaactcc aaaccagttg
     1981 atttaccgga atggcacact tttctacggc ggaaacctgc tcgtttcgaa tgtcggccca
     2041 gcgttggttt ctgggaacag ctttcaattg ttcaacgccg cgagttatgg cggggctttt
     2101 tctgaaactc tcccatccct gtcgcccggc ttgatctggc aggacgactc cctcgttaat
     2161 gggacgtttg tagtgactgg cacgagcaca ggcggtaccg gctctcccac actttcctat
     2221 tccgtgagcg gaaatcagat gactttgtcg tgggacgccg ccacctatcc cggctacgtg
     2281 cttcaatcca gcgccttcct gggtgctacc gcgagttgga gcgatgttgc cgggggcaac
     2341 atcgctcctg tacccattac gatgtcgggt gcgaacacct tctatcgttt gcaccatcca
     2401 taagtgtggc gcgagagcat tgcccgttgc aggtaattgc aaaccaaata aaccataacc
     2461 taacaatcaa ccatatatct aacatgagca ataaattaac agggatttgt cttctaactc
     2521 ttggcacggc tgtctttatg gctatgcctg ctgtcaaagc cgcaaccttc actgctgccg
     2581 caacgggcaa ttggagctcc ggcgccacgt ggggcaattc gggcaataat gtggtagggt
     2641 caggcgtgcc tggtgcttct gacagcgccg tcatcgtttc cggtaaaacg gtcacttatg
     2701 acagtagcgc gcccgcgatt gcgggaacgg ttgaagtggc cggatcgatg gtggtatcca
     2761 agagcggcgg gacatttggg gattttacga ttgattcggg aggaactttc tctttcggag
     2821 gccaagtgcc ggtcacgttc agtggcaact tcaccaacaa cgggaacatg aatatttcaa
     2881 gccagggcca gactgcgctg accacctata gcgggattgg caatgtaatt gccggaaacg
     2941 ttaccaacca gatagcaaac gtaacgggga cttataccaa tatcggcatg ctggtgaccg
     3001 gtgtcaatgc gaccggcaca ctcaacttgg aaggatccgg gacattagtg aaccttggca
     3061 cgattttata caactgcaat aaggcaccca ccgtcaccac cttggactgt tcagcgcctg
     3121 gcaatctctt tatttgggcc ggtgttaaca tgtccagcac cacgcccaaa gccacggctt
     3181 attataattt gatcataggc aatactggca gcgccgggtt tcccttggca ggcgcggggc
     3241 tttccatcgc caacaatctt accgtgacaa attccgggaa catcacttcc tggccagcca
     3301 acggtgccat aggtggcacg ctgagttatt tcaccacgtc cactacagcc gccgcaagtt
     3361 tcccgaccgc attttcggtc ggtggttttg tccagaccaa tggtgtcctg gccatggctg
     3421 caaatgatgt cctcacagtg accgggaccg gagcgggcac atggactcgc acgggcggca
     3481 ctttcaacca aaattcatcg agcatggtca aattcaccgg cgccgctcca gtcatcggcg
     3541 ggaccgtcac caataatttt gccaaccttt atatagactc aacggcggcg aatgcgattg
     3601 cgtccacgaa cctcaacgtg accaacacat tgaccatcgt ggcgggggca tcgctggacg
     3661 tgaccgcttt caacgggggt gccatgacga tgttgaacgc ggaatccctc ttgaacggtg
     3721 gcatgctggt tggaaatgtg actgcgccat caagcgccaa aatctttgcc gggttggatg
     3781 gttcctatgg cacgaatatc atctcgggaa atctcaccat gtcgtccacg aacagcagca
     3841 tcaacttgga cgtcaacagc agcgcaacgg cgccgaatga tctgctgatg gtgggtggcg
     3901 cgttaagctt gaacaatact ccgatcaagt tgaaagcgcc cagcgcgggt gcggcaatag
     3961 atacttccgc ggattatacc ctggtcacgg ctgggagcat tactggagtc cctctgctca
     4021 cgtggatcac ggccccggcg aatgcgacaa attactcctt ggttacgtcc tccactacca
     4081 tggcattgca ttatgttggc gcggtgattt caaatccgcc caccctggcg tcatccgtaa
     4141 acggaaacca aatgacgttt tcttggaata ccgccactta tcccggcttc tttctccaaa
     4201 cgagtccgac tcttgctccc ggcagttgga cgccggtgcc cgcaggcgat acaagtccag
     4261 tcctcatcac gatagacccg agtacgaacg catttttccg gttggctcag taagcgttcc
     4321 ataatgaaac cgttcttggc cttgtttttt gcagttgcct tgtcaacgtt gggcagggca
     4381 gaaaacgcca cagtcgtcga gtcctttgaa aatggaattg acaatgtgag catcgtcacg
     4441 aacgcgggag gccgcggggc gctttcacca ccaggcgtga gcttgagtct tcatgttaag
     4501 gccggtgaag gcgatgccag cctcacacac ggcaacaaat cgttgaaagt ggaattgagc
     4561 ggcaaggaaa agttcggtgc ggacttccga atcaactttt ccgccgaggc ttccgagcag
     4621 atccgcaagg cggtcgcatc gccggacatc gcccggtaca tcctgcggta cgatgtgatt
     4681 ttcccctcca tggacgactt cgcctatttt aacagcattt tgtatttggg cgactgccgg
     4741 gatgctttga tcggggcgag tggcaagagg agcatgtccg tggcgctgga tcttttgacc
     4801 ggcctgcccg aatccggtcc gatcacgctg gtgatggccg acgattttgc ttatcgggcc
     4861 gattcaaaac tcgcctcgtc aaacgtgacg ctttacatcg acaacatacg catcgtggac
     4921 acctacgcgc ccggctgcaa gccggtcgtg tatgtcctgc aatcctttga agacaccaat
     4981 gacccgattg gtggcatcac aaatttttcc gattgggaca acggcacaaa caccacccgc
     5041 accaggttca agtcttacgc ggcttcgggg ccggatgatc tgcgagtgac tgaggggaaa
     5101 aatgcgctgg aggttgagaa caacgcgccg cttccatggc acgccgattt tacgcttccc
     5161 ttcaacaaca cgaagctagc ggaagtgctc aagttggacg ctcccaccga gcaacagccg
     5221 actgcagaag aactttccca ttacacgttg cgctgggaca ttacatatcc cgacatgacg
     5281 aacgaatgga tgaattcgac ctttcacacg atggaaacat tcatgcctgt cattcaagtc
     5341 cgtcatgaaa aaccaaccaa ccaaagatta acgtattcct tgacgttgga tcaggttcgg
     5401 tggggcaagc gaaatgatat ttacccggtg ctgacattca ttaccgaagg gccgcaaaaa
     5461 acccacaata tcaagattta ttacgataac tttcgattga ttgaaacaca caaatcgccc
     5521 aagtccgacg tggcgggaag agaaaaataa gaggccggga ttttgggcgg gcaagcgagc
     5581 ttttgtcatt tgtataaatt atcgggattt ctttagagcg tataatggct ctgaactttt
     5641 tcttgctgga accgctcgtc tgtgagggtt ttcaaaaagg cgacgagcgc ctgtttgtcc
     5701 gcctttttca acggcacgcc gccgtcagga tgcttcgcga ggtttggatc gagcgtcgcg
     5761 ctgcgtttca ttccggtgca ataatgttcg accgcctcct ccaaagtcct gaaccgtccg
     5821 tcgtgcatgt aaggcgcggt gagcgcgatg ttgcgtaatg acggcacggc gaattttccc
     5881 tgatcaccgt cgcgatgcgt cactgcgtag cggcccgtat ctgagaattc cgcgtcgagc
     5941 ccgttgttgg cgaacgtctg gctttgaaac aacgctccgc catggcaatg aaaacaatca
     6001 gcgccgaatt ggccccgacg aggatcgtat tcggttgaaa ataattgaaa tccgcgctgc
     6061 tcttccgcgg tgagctgttc ctgtccgcgc aaggcgcggt cgaatttgga atcaaacgac
     6121 gtgaggctca gcagatactg ttcgagcgcg aggccaattt tttcaggcgt gatttccggc
     6181 gtgccaaagg ccgaagtgaa ggcagcggcg cagccgtcct ttgcagccag ttt
//
LOCUS       DASZMM010000224         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4218104, whole genome shotgun sequence.
ACCESSION   DASZMM010000224 DASZMM010000000
VERSION     DASZMM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4218104"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VG754_05325"
     CDS             <1..1234
                     /locus_tag="VG754_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWF18664.1"
                     /translation="YAWRGANFQNILKFPERHPSAQIYKIETNYRSTPEILQLANAAI
                     AANVGQFTKELAPARKSGAKPVLVTCLTASEQAAFVAQRILELREEGVNMNEMAVLYR
                     SHFHALELQLELTRRNIPFNITSGIRFFEQAHIKDATAYLKFVTNPRDEIAFKRMVQL
                     LPGIGGKGADKLWLNFNAAFSASAAKPGTEAKEPASRSPLAAALQACANSVPKKAAVA
                     WAQLVATISQLETAPVRGDSGKMLRLVVEAGYEDYLQENYANYRNRLDDIEQLAIFAQ
                     QFPTAEDFLTQLALLTNLEGESDQPASNDTEQVRLSSIHQAKGLEFDVVFLIMLCDGL
                     FPASRSLDTPEGEEEERRLLYVAITRARNEIYLSYPLMRMLPGGSGDAMQQPSRFLNE
                     IPRELVEEWNLKPVHPYG"
     gene            complement(1294..1599)
                     /locus_tag="VG754_05330"
     CDS             complement(1294..1599)
                     /locus_tag="VG754_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18665.1"
                     /translation="MKLTIFFIYGFLFSVVFLISALLWHAQMQDVYFVCHQKGVIADF
                     VPPFVQPGESGGFYIKPPHVVYVIWIVYLGCTVAIPAVCSWLILRMHRRALKKAWLL"
     gene            complement(1909..2439)
                     /locus_tag="VG754_05335"
     CDS             complement(1909..2439)
                     /locus_tag="VG754_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWF18666.1"
                     /translation="MNTASARLLVFASGRLACIKIAGRANFTSSLDFKLLIHELSDKA
                     VIRFVLDLSECVLMDSTFLGVLAGFGLEMSKSQNGQSIELMDATPRIVELLENLGVLH
                     LFKVITSPCPDLPNDGATPMAVPGTHATREEITRTCLEAHKTLMEIDAANIPKFKEVA
                     QFLAEDLKKLKGNSGT"
     gene            complement(2730..>3309)
                     /locus_tag="VG754_05340"
     CDS             complement(2730..>3309)
                     /locus_tag="VG754_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWF18667.1"
                     /translation="NARSLANEVEKLSLFIGDRPEIEVADVMTVCTRNKSARAFALGD
                     ALGDRDLPRLLRTLDEELWELKLDRQKSEIGLLYGLIGKVRAMILLKEMLREGWIKPD
                     GDYNRFKAQLERVPAAQLPEDKRYNPLALNAYVLFKALPQCRRYSQAELISAMELLLR
                     CNQRLISSSLDEALILQQTLVEIVEKPAAGKR"
BASE COUNT          787 a          913 c          864 g          745 t
ORIGIN      
        1 ttacgcatgg cgcggcgcga attttcaaaa catcctgaag ttcccggaac gccatcccag
       61 cgcgcagatt tacaagattg aaaccaatta ccgcagcacg ccggaaattt tgcaactggc
      121 caacgccgcc atcgccgcga atgtgggcca gttcacgaag gaacttgcgc cagcgcgcaa
      181 atcgggcgcg aagcccgtgc tcgtcacgtg tttgaccgct tccgagcagg cggcattcgt
      241 cgcgcagcgg atcttggagc tgcgcgaaga aggagtgaac atgaacgaga tggcggtgct
      301 ttatcgctcc cattttcacg cgctcgaatt gcagctcgaa ctcaccaggc gcaacatccc
      361 gttcaatatc accagcggca tccgtttctt cgaacaggcg cacatcaagg acgcaaccgc
      421 ttatttgaaa ttcgtgacca atccgcgcga tgagatcgca ttcaagcgga tggtgcaatt
      481 gctgcccggc atcggcggca agggcgcgga caaattatgg ctcaatttta atgccgcatt
      541 cagcgcgtcc gcggcaaagc ccggaacaga agcaaaagaa cccgcatcgc gttcgccgct
      601 tgccgccgcg ttgcaagcct gcgcgaattc ggtgcccaaa aaagccgctg tcgcatgggc
      661 gcaactggtc gccacgattt cacaactgga aactgcgccc gtccgggggg attcgggaaa
      721 gatgctgcga ttggtcgtcg aggccggtta cgaggattat ttgcaggaga attatgcgaa
      781 ctatcgcaat cgcctggacg acatcgagca gcttgcgatt tttgcgcagc aatttccaac
      841 ggctgaagat tttctcaccc aacttgcgtt attgacaaat cttgagggcg aatcagacca
      901 gcctgcttcc aacgacaccg aacaagtgcg cctttccagc atccatcagg cgaagggatt
      961 ggagtttgat gtcgtgtttc tcatcatgct ctgcgacgga ttgttcccgg cttcgcgctc
     1021 gctggacacg ccagaaggcg aggaggaaga gcggcgtttg ctctatgtcg ccatcacgcg
     1081 cgcccgcaat gagatttatc tcagttaccc attgatgcgg atgctgcccg gaggcagcgg
     1141 cgacgcgatg caacagccat ctcgtttctt gaatgagatc ccgcgagaat tggttgagga
     1201 atggaatctg aagcccgtgc atccgtatgg gtgattctaa tcggccttgc actaaatgct
     1261 tcaatggtca aaattgcttg ttagacatct cggtcagagg agccacgcct tcttcaacgc
     1321 acgccgatgc atccgcaaaa tcagccagga gcaaaccgcc gggattgcca ccgtgcagcc
     1381 gagataaacg atccagatga cgtaaaccac gtgcggcggc ttgatgtaaa acccgccact
     1441 ttcacccggt tgcacgaacg gcggaacgaa atcggcgatc actccctttt gatggcagac
     1501 gaaataaacg tcctgcatct gcgcgtgcca gagcagcgcg gaaatcagga agaccacgga
     1561 aaagaggaat ccgtagatga aaaatatggt cagcttcata aatttcgcct gcaacttcgt
     1621 tttccggtgc gaacacattg cccacccggt tgtgctgaaa caatcaccgt tatatcctca
     1681 aactgttttc gtgacaatag aagttatgtg ggcactcgcc gatgggcaca tcttcatcct
     1741 gttctggagc ggggactgga aagtccccgc gacccgcagg ctggaaagcc tgcgccacgt
     1801 agcgcagaca ttcttgtctg cgggttcagg cgactttcca gtcgcctgat tttaggtcca
     1861 gtttcctcga tcaataatag tcccacacgt gacctcaaca tccgcgagtc atgtcccgga
     1921 attacccttg agcttcttca aatcttccgc gagaaattgg gcgacttcct tgaacttggg
     1981 aatattggcc gcgtcaattt ccatcaaagt cttgtgcgct tcgaggcagg tgcgggtgat
     2041 ttcctcgcgc gtcgcgtgcg ttccgggaac agccatcggc gtcgcgccat cgttcggaag
     2101 gtccggacat gggctggtga tgaccttgaa aagatgaagc acccccaaat tttccagcaa
     2161 ttccacgatg cgcggagtgg catccatcaa ttcaatggat tgcccgtttt ggcttttgct
     2221 catttccaag ccgaaccccg ccagcacgcc aaggaacgtg ctgtccatca acacgcattc
     2281 ggaaagatca agcacgaaac ggatgaccgc cttgtcgctc aattcatgga tgagcaattt
     2341 gaaatcaagg ctggacgtaa aattcgcgcg ccccgcaatt ttgatgcagg ccagtcggcc
     2401 cgatgcgaaa accagcaacc tggctgaggc ggtgttcata aagcgctcat tcggtcgcca
     2461 ctcaatatga atttatacac gatgacagtc aagcacgggt ggggttggtt aaatagttaa
     2521 attgttaaaa tgttgcatgg gaatcggcca tcttgggtta ttgaaggctt ttgtaaccgt
     2581 ggcaaaaacc agatgagcgg cagaaaatga gttaagagtt ttttattagg aaatcatgaa
     2641 atcaggaaca aaattgttga ggaaacatcg tcttttcctg ctttcctaat tctatttccc
     2701 acaggatggc tctgtaacga tttgaccatt caccgcttcc cagccgccgg tttttccaca
     2761 atttccacca gcgtctgctg caaaatcagc gcctcatcca aactgctgga aatgaggcgt
     2821 tggttgcatc tgagcagcag ttccatcgcg ctgatcaatt cggcctggct gtatcggcgg
     2881 cattggggca gcgccttgaa aagcacgtag gcattcaagg cgaggggatt gtaacgcttg
     2941 tcttccggca gttgtgccgc cggcacgcgc tccagttgcg ccttgaaacg gttgtaatcg
     3001 ccatcgggtt tgatccatcc ttcgcgcaac atttccttga ggagaatcat cgcgcgcacc
     3061 ttgccgatca atccgtaaag caaaccgatt tccgattttt gacggtcgag cttcaactcc
     3121 cacaattcct catcgagcgt gcgcagcaga cgcggcaaat cgcggtcgcc caacgcgtcg
     3181 cccaatgcga acgcgcgcgc ggatttgttg cgcgtgcaaa cggtcatcac gtccgccact
     3241 tcgatttcag gacggtcgcc tataaacaac gaaagttttt ccacctcatt ggccagcgaa
     3301 cgcgcattc
//
LOCUS       DASZMM010000225         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4218471, whole genome shotgun sequence.
ACCESSION   DASZMM010000225 DASZMM010000000
VERSION     DASZMM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4218471"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VG754_05345"
     CDS             complement(<1..1078)
                     /locus_tag="VG754_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF18668.1"
                     /translation="MLEKASFTGQPSTMGRYTVALLSVAAALFVHWMLDPALREHLGF
                     VTVYGGVAFAVWYGRWKPALAAAILGWLGASYFFFIPGHVIAGDTKTLARLAGYIFSC
                     VIIIGFGELLHRTRDRAEAERDERKLAQQAEARQRELLTTSLKSIGDAVILTDAQGRV
                     TFLNAEAERLTAWTSAEALGRPLPEVFRIANEHTRQTVENPVEKVVRLGSVVGLANHT
                     ILLTKDGREIPIDDSAAPIREPGGPLFGIVLVFRDFSERKKAQEADARLAAIVAHSGD
                     AIIAKDLKGIVRSWNAGAERLFGYRPEEIVGKPVTILFPQDRLEEEDHILDNLRQGRP
                     VERFETVRVAKDGRHIPVLASISPI"
     gene            complement(1309..1479)
                     /locus_tag="VG754_05350"
     CDS             complement(1309..1479)
                     /locus_tag="VG754_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18669.1"
                     /translation="MIWRKRNKEQERFYLLPGMGGRASRRKHKVFLVWSIVAGLTVSI
                     LFAVALFFLNRR"
     gene            complement(1599..>2694)
                     /locus_tag="VG754_05355"
     CDS             complement(1599..>2694)
                     /locus_tag="VG754_05355"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWF18670.1"
                     /translation="YRGIPIEELADKSTFVETAYLLIYGELPNRQQLRHFSDLLTNNQ
                     YLHEDMTFHFQGFPSAAHPMAMLSAMINAASCFDDGLMHWQFGKQTEFEVYAARLISQ
                     VRTLAAFAHRKSLGLPFIYPKASYKYTANFLHMLFSMPYEDYDLKPEVVHALDLIFLL
                     HADHEQNCSTSTVRMVASSQANLFASAAAGVCALWGPLHGGANQAVIEMLQEIHATGD
                     DGSKFIAAAKNKNSGKRLMGFGHRVYKNFDPRAKIIKHACDELLKVLNVNDPLLEIAK
                     KLEETTLHDSYFIERKLYANVDFYSGIIMRAIGIPLEMFPVIFAIGRMPGWIANYKEI
                     METPNGRIYRPRQIYEGRTESHYVPIEQRK"
BASE COUNT          613 a          809 c          683 g          589 t
ORIGIN      
        1 tgatcggtga aatgctcgcc aaaacgggga tatgccggcc atccttggcc acacgaacag
       61 tttcaaaacg ctcgaccggg cgaccttgcc ggagattatc aaggatgtga tcctcctcct
      121 cgagccggtc ttgcggaaac aaaatggtga ccggtttgcc cacaatttcc tccgggcgat
      181 agccgaacag ccgctcggcg cccgcattcc atgaccgcac aatgcctttg aggtcctttg
      241 cgataatcgc gtcgccggaa tgggctacaa tcgccgcgag acgcgcatca gcttcctgcg
      301 ctttcttgcg ttctgagaag tcacggaaca ccagcacgat accaaacaac gggccacccg
      361 gttcgcgaat gggcgcggcg ctgtcatcaa tcgggatttc gcggccatcc ttcgtcagca
      421 aaatggtatg gttggcaagg ccgaccaccg agccaaggcg aaccaccttt tcaacaggat
      481 tctcgaccgt ctggcgcgtg tgttcgtttg cgatgcggaa gacttcgggc aaaggccggc
      541 ccagcgcctc ggcgcttgtc cacgccgtca aacgttccgc ctcggcattc aggaaggtga
      601 cgcgtccctg cgcgtcggtc aaaatcaccg cgtcaccgat gcttttcaag ctcgtggtca
      661 gcagttccct ttgacgcgct tcggcctgtt gcgccagctt gcgctcgtcg cgctcggctt
      721 ccgccctgtc ccgcgtgcga tgcagcagtt ctccaaatcc gatgatgatg acgcaggaaa
      781 aaatataacc cgcaagccgg gccagtgttt tcgtgtcacc cgcaatgacg tggccgggta
      841 tgaagaaaaa ataacttgcc cccagccagc caaggatggc cgccgcaagc gccggtttcc
      901 atctcccata ccagaccgca aatgccacgc cgccatagac agtcacgaaa cccagatgct
      961 cgcgtaaagc agggtccagc atccaatgca caaacagggc ggcggccaca ctcaaaagtg
     1021 cgaccgtata acggcccatc gtggaaggct gtcctgtaaa actggctttc tctaacattt
     1081 atcgaataat tccggctgct tttcttgcat gatgcaagtc aagccgtgaa tgcggcgctt
     1141 ctttagaaag cttaattact taatccaatt accccgtttt cgcaaccgga taagtcgaaa
     1201 aaaaacgagt tacagcgaag aacctgtttt ggcgattccc ctataaaaag ggttcgtggg
     1261 gaaggctttt tgtcactcag ccatcaatgt tcacctcgca ctgcttcatc aacgccggtt
     1321 tagaaaaaag agggccacgg cgaacagaat ggataccgtc agcccggcga cgatggacca
     1381 taccagaaag accttgtgtt tgcggcgcga agccctgccg cccattcccg gcaacagata
     1441 aaatcgctcc tgctccttgt ttcgtttccg ccaaatcatt ccggttaaaa aatcacgttt
     1501 gggtcgggcg ccattttgtc cggttctctt cgagaatcaa gaatggaatt tggggaaatg
     1561 tctctcaccg ttcaacacca gccttcgctc cgcgccattt atttcctctg ctcgattggc
     1621 acgtaatggc tctccgtccg gccctcgtaa atctggcgcg ggcggtaaat gcgtccgttc
     1681 ggcgtttcca tgatttcttt gtaattcgca atccagccgg gcatccggcc aatcgcaaaa
     1741 atcaccggga acatctccag cggaatcccg atggcgcgca tgatgatgcc gctgtaaaaa
     1801 tccacatttg catacagctt gcgctcgatg aagtaggaat cgtgcagcgt cgtttcctcc
     1861 agcttcttgg caatctccag caacgggtcg ttcacgttca acaccttcag caactcgtcg
     1921 caagcgtgct tgatgatctt ggcgcgcgga tcaaaatttt tgtaaacgcg atggccgaaa
     1981 cccatcaacc gtttgccgct gttcttgttc tttgcggcgg cgatgaattt cgagccgtcg
     2041 tcgcccgtcg cgtggatttc ctgcaacatc tcgatcaccg cctggttcgc gccgccgtgc
     2101 aacgggcccc acaacgcgca cacgcccgcc gccgccgacg cgaacaaatt cgcctggctc
     2161 gacgccacca tgcgcacggt cgaagtcgag cagttttgtt cgtggtccgc gtgcagcaaa
     2221 aagatcaggt ccagcgcgtg aaccacttcc ggtttcaaat cgtaatcctc atacggcatc
     2281 gaaaacagca tgtgcaaaaa gttcgccgtg tatttgtacg acgccttggg ataaatgaac
     2341 ggcaagccga gcgacttgcg atgcgcgaac gccgccaacg tccgtacttg agaaatcaat
     2401 cgcgccgcat aaacttcgaa ttccgtctgt ttcccgaact gccaatgcat caacccgtca
     2461 tcaaaacagc ttgccgcgtt gatcatcgcc gacaacatcg ccatgggatg cgccgcgctg
     2521 ggaaagcctt ggaaatggaa cgtcatgtcc tcatggagat actgattgtt cgtcagcaag
     2581 tccgaaaaat ggcgcaactg ctgccggttc ggcaactccc cgtaaatcag cagataggcc
     2641 gtctcgacga acgtggactt atccgccagt tcttcgatgg gaatgccccg gtaa
//
LOCUS       DASZMM010000226         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3391092, whole genome shotgun sequence.
ACCESSION   DASZMM010000226 DASZMM010000000
VERSION     DASZMM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3391092"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="VG754_05360"
     CDS             <1..1422
                     /locus_tag="VG754_05360"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HWF18671.1"
                     /translation="GFCLTGLVVFCVAFDLISSLNRIQEHQLLLRDVVELYLVKLPGL
                     LVFILPVTLLLSLLCALTNHTRHHELVAMRAAGIGLWRLCVPYIVVGFLLSLVVFALN
                     EMFVPDSDARLAQIMDRRQRQNAAHSDASVQHTLGFPNSRDSRNWVWDSFNFNTGVMT
                     NPKIDWEENGVQWTLFARRAERVNGVWTFYGNLFFSTNDLINPDSLAARLTHPTNALS
                     QFLAGHLSTNTETLLAHCTNAGTNLELKRALTDDLNRIIRKGPVYETARFAAVKLPAD
                     MVESAGLYHRGADLLMFNRTLLIEAWPDQFSKNSLYSVRVTTKAGALAHSTNVLTMPQ
                     FTETPRDFAIEAKFNQRFQALAAESAGIPIADILDYLKLHPNLGQKNKRWLLTQLHGR
                     IASPWSCLVVVFIAIPFGAASGRRNIFAGVAGSIVVCFAYFILLKVGLWLGTSGMLPA
                     WTAAWLPNAVFGISGFWLMLRVR"
     gene            1419..>1903
                     /locus_tag="VG754_05365"
     CDS             1419..>1903
                     /locus_tag="VG754_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWF18672.1"
                     /translation="MSDELTELKARYARLDLLRQVDHIIHSTLEPQEALQLILEQAVR
                     VMRASSGSVVLLNPTSGLLEIHASMGLPPKAADVKLRLGEGLTGWVARKGQAIRSGDV
                     SQDSRYIQLRPNVGSELAVPLVVNGEVRGVLNVDSDRKDAFSASDQELLESLALSASK
                     V"
BASE COUNT          361 a          553 c          541 g          448 t
ORIGIN      
        1 ggcttttgcc tgaccgggtt ggtggtgttc tgcgttgcat tcgatttgat cagcagcctg
       61 aatcgcatcc aggaacatca actgctcttg cgcgacgttg tcgagttgta tctcgtcaaa
      121 cttccgggac tgctggtgtt catcctgccc gtcacgctgt tgctctcgct gctttgcgcc
      181 ctcaccaatc acacgcggca tcatgaactc gtcgcgatgc gcgcggcggg aatcggtttg
      241 tggcggcttt gcgtgcctta cattgtcgtc ggttttttat tgagcctggt ggtgttcgcg
      301 ctcaatgaaa tgttcgtgcc ggacagcgac gccaggctgg cgcaaatcat ggaccgaaga
      361 caacgccaaa atgccgcgca ttccgatgct tccgtccaac acaccttggg atttcccaac
      421 tcgcgcgaca gccgcaactg ggtctgggac agcttcaatt tcaacaccgg cgtgatgacg
      481 aatcccaaaa ttgactggga ggaaaacggt gtccaatgga ccctttttgc ccgtcgcgcc
      541 gagcgcgtca acggcgtctg gactttctat ggcaatctct ttttttcgac caacgacctc
      601 atcaatcccg attcgctggc ggcccgtttg acgcatccaa cgaatgccct gtctcaattt
      661 ctcgccgggc atctttcgac caacacagaa acgcttcttg cgcattgcac caacgcggga
      721 acgaatctcg aattgaaacg cgcgctgacc gacgatttaa accgcatcat ccgcaaaggg
      781 ccggtctatg agactgcccg gttcgcagcc gtgaagctgc cagcggacat ggtggaaagc
      841 gccggactgt atcaccgtgg cgccgacctg ctgatgttca atcgcacatt gttgatcgaa
      901 gcgtggccgg accaattttc gaaaaattcg ctctattccg ttcgcgtgac gacaaaggca
      961 ggcgcgcttg cgcattcaac gaacgtgctg acgatgccgc agttcaccga gacgccgcgc
     1021 gatttcgcca tcgaagccaa attcaatcaa cgtttccagg cgcttgccgc cgaatccgcc
     1081 ggcatcccca tcgccgatat tttggattat ctcaagctgc atcccaatct tggccagaaa
     1141 aacaaacggt ggcttttgac gcaattgcac gggcgcatcg cgagtccatg gagctgtttg
     1201 gtggtggtgt tcatcgcgat tccgtttggc gcggcgtcgg gccggcgcaa tatttttgcg
     1261 ggagttgcgg gcagcattgt ggtctgcttc gcgtacttta ttcttttgaa agtcgggcta
     1321 tggttgggga cgagcgggat gctgcccgcg tggacggcgg catggctgcc caatgccgtg
     1381 ttcggcattt caggattttg gctgatgctt cgcgtgcgat gagcgacgaa ttgacagaac
     1441 tgaaggcgcg ttacgcccgg ctcgatttgc tgcggcaggt ggaccacatc atccattcca
     1501 cgctggaacc gcaggaggcg ctgcaactca tcctcgaaca agccgtgcgc gtgatgcgcg
     1561 cttcgagcgg ttcggtggtg ctcctcaatc ccacttccgg cttgctggaa attcacgcct
     1621 cgatgggctt gccgcccaag gccgccgacg tcaagttgcg tctcggtgaa ggtttgaccg
     1681 gctgggtcgc gcgcaaaggc caggcgatcc gcagcggcga tgtttcccaa gactcgcgtt
     1741 atattcagct tcgcccgaat gtcggttccg aacttgccgt gccgctcgtc gtgaacggcg
     1801 aagtccgtgg cgtgttgaat gttgattccg accgcaaaga tgcgttcagc gcgtctgacc
     1861 aggaattgct cgaatcgctc gcgctctccg cgtccaaggt cat
//
LOCUS       DASZMM010000227        11556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2565331, whole genome shotgun sequence.
ACCESSION   DASZMM010000227 DASZMM010000000
VERSION     DASZMM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565331"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="VG754_05370"
     CDS             complement(<1..1530)
                     /locus_tag="VG754_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18673.1"
                     /translation="MAASTNYWVAPTAAGTGNGSSVANAAYYLNISFWSTVQSQLQSG
                     NVTVNFQDGAYNAGSLSLTNMGNPLYPLTLAAVNPHGPVFSPSANILQLYGCQNFQVN
                     GLVLTGPAPYWGIYCIPNGSNPCRNIEISNCWFLNLTNAYYAAIGLLNGTRDIQVLNC
                     NFTNITAGSHQHMIYAPHDIENVLVSNCVFQDCLADYVRFRDDSEYCVVKNCNFISTT
                     SASAWPFVSAELYNETNADSAGDEFFGTYFQVSSNSFTYQASGGSGPYAALHFSDNGY
                     SPQSYDCDLTSSQASQLSSGTTSFQQSFLQTNMGLIASGIKMFGNTYNSRVAYHMDYA
                     YTWDSIQTNGGWQGTIKLDNVPDASGTPLGPPPVFRNADFNRRGLLLTSISVGLKDYE
                     CLFRDWQCSPKYTSIHLSSNFYGTNNALRFDAASSQYVYQWISSPGPTWTMDCLFAIG
                     SSFTGTGVKFKLDLIHNDLTGSKVSLGVDNLGRFGIYNGGTFTILSGLGTVAFSVDNT
                     GD"
     gene            complement(1632..2291)
                     /locus_tag="VG754_05375"
     CDS             complement(1632..2291)
                     /locus_tag="VG754_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF18674.1"
                     /translation="MAIAVSIVEDDAGLCESICRFIQGAPGFQCLGAYASAEEALADV
                     PNRPPAVVLMDINLPKMSGIECVKHLKEKLPGLQIIMLTVYENSDRIFEALAAGACGY
                     LVKNTPPEKLLEAIEDIAKGGSPMSSHIARKLVQAFQPAVCAVPLIEQLAPREQQVLE
                     LLSKGRAYKQIAGEMNLSMGTIRTYIRRIYEKLHVNCRTDAVVKYLHATGSERRPISR
                     A"
     gene            complement(2367..5363)
                     /locus_tag="VG754_05380"
     CDS             complement(2367..5363)
                     /locus_tag="VG754_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWF18675.1"
                     /translation="MIALPATEFNPTLWQTEEGLPNNNVLSIAQTQDGYLWVGTREGL
                     ARFDGIKFTPLMVTRSSPQPTINALCASDDGSLWIGTEQHGLYCYKGDALAHYETALT
                     NDGVVQIKQSGTNEIWVAFHDGLAQIRQGRLHFEESFGSATNVLLSLYPDRNGGLWIG
                     ATRSVQYLKDEKALTYKVANNLVLRAIRALYEDQDGTIWIGNNRGLTSLRDGTFTHYS
                     KGNGPPGIVTALLRDHGGNLWVGTQGGLSRFIDANFYENQAPEGTSYRVYSLFEDREG
                     DIWVGSETGLTRLTTKPFTAYTKQSGLTQNATTAVCATRDGSVWIGTWGGGLDRLKDG
                     IITAYARSNGLSSDFVVALMPGDFDSGLWVGTAYASGSAGAYSPGLNRFKDDKILNEN
                     AEVSGLGSSLTCLLEDRKGAMWIGSHMGLSRFEREDQLANGGSIELSNCAVNALCEDH
                     VGNLWIGTDTGLRRWNGTVVEKGTIPGADFTKRVLSIYEDVEHVLWIGTAADGLARLT
                     SSNFSVISARRGLAADSIYAIMEDGEHNLWMNSGKGIFRVAKNQFAELADGKINRLNC
                     ISYGKAEGVLGTGQAQETTQPAACKDSDGRFWFRTTQGVVVTDPARIVPNRLPPPVVI
                     ETVLADGIPVYSSSSIHVTNAMTGGLPAKSLPIKLKIPPSHGELEIQFTALSLQAPEK
                     NRFKYKLSGVDSDWVDAEGRRTVAYKRLPPGSYVFQVIACNNEGIWNESGATVALVLR
                     PQFWQTWWFLGLSSLLAIALIGIATRYATRRRMQRRLERLEYQHAIERERTRIARDMH
                     DELGAKLTRISFQGAMALRSLDDRTEAERQVGSMAETARDLVASLDEIVWAVDPGNDS
                     LENLANYISRYASEFCADGPLSCDLIIPAKLPDYKLLTDVRHNIFLAVKESLNNALKH
                     SGGTQVQVSMKTKDHELEVVIADNGHGINQPHPAESNGRTRRTGRGLSNLRERLETIN
                     GRCEVESLPGQGTRVRLIVPLNSVPQ"
     gene            complement(5714..6589)
                     /locus_tag="VG754_05385"
     CDS             complement(5714..6589)
                     /locus_tag="VG754_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18676.1"
                     /translation="MKKRLAITDEAADAFTLIELLVVIAIIAILAALLLPALSAAKQS
                     AKGTGCINNKRQIQLAYNMYVGDNADQLPINEVGNSTGGWVTSYLDWTAGASSPNTNV
                     ANLSAGLLGSYTSQSISCYLCPADIYLSPAQRAAGWSGRIRSVRVNGYLAHKPVEPTW
                     DSTSWGPNFTQNWKMSDMTSPSSLWVFIDAHPDTGDAGGASHPYDGVFSFPPGGVTQN
                     GNCNWNDMPASYHKGKYCGFSFADGHAEIHKWLSHSTIIPVTYNGDLYGTVVYPNDSQ
                     DIIWTFHHAYNSGVN"
     gene            complement(6609..9161)
                     /locus_tag="VG754_05390"
     CDS             complement(6609..9161)
                     /locus_tag="VG754_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18677.1"
                     /translation="MKHILATALSATIFFAVFSLQAASLYWDPNATTGSALGGTGNWD
                     SVGTFWYDGTQDNIWMNANGDDAFFTGTAGTATLQTNITAGDLYFTNTTGSYVLTNAT
                     GAEVLTLLNGTIDTGGGEHTIAAPIANSTILTKNGLGRLHLTTNNASTITGSVVINQG
                     DISVENNYAVGQQGAPVTVSNGAALVLNGDANGSTNGLTSFTSSVTINGSGITNSGAL
                     RNLSGVTTFYGQIILGQNDSMIYVDTGAALVYDGENGPLTDNGNNYNLIISASGTGNF
                     DMGATSIGGTLIVEGPASCYAYLNSIAPTVWKSTYVGPGATFYVENNNSFGANVNPAS
                     LMTTNVLLDGSAIISTGSYSMWANDGITVTTNGGTLTVNSGTWTTCNIYSQSNGSVTL
                     GGSGSMRPGGAAGASAGTINLGTGTLTKSGSGDCNMGYANPSLEIYSNLVVNGGSLTF
                     NYDQSSGQVSSLGTLPSTLNASNIIFNGGSMHVGHSTTIGATRGIYVASGGGTIEDVV
                     SSGGTVTINSPISGPGSMNFPNGHAGVTSAVTLAANNTYTGTTTVGASSLVTVGAGGT
                     TGTLGTGNTTDNGTLTFNRTGSYSYGGVISGSGWVTKTASGTVTLTGANTYTGNTTNS
                     GGTLLINNTGKSGTGTGSVVISSGATLGGSGFISGAVTINSGATLAPGPAASSVGTLT
                     ISNNLTIAGNAAIAINKSLSPSNGAVAVTGTLANSGSGTLTVTNLGPALVVGDTFQIF
                     SEPLTGNAMAVSGGKVIWNNNLAVDGSISVARLPIPVITGFSFSGSNIVFAGTNGYPL
                     DGFKVLTSTNVAAPLTNWTTFSTGSYNASGNFAVTNAVTNSVPAQFFIIKSN"
     gene            complement(9531..11438)
                     /locus_tag="VG754_05395"
     CDS             complement(9531..11438)
                     /locus_tag="VG754_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HWF18678.1"
                     /translation="MNILRHLLRALFAFMVLWAMPVCAATNMVSPQRVQQIAAMLSPL
                     TASFGVPITNRAVWAKMADNPAVRPIIPEAEKLAEEPIPEMTDDLYLDCLRTGNRDRG
                     QKVQLARSSRLCVLTLAECFEDRGRFLKPLQKIIEAMCAERSWTYPAHDLRLEVFDGK
                     LMRPDLRATTLAFDLATADCLLGDKLPQATRQLLRENIRKRVLLPFRGTVEGRLKDAY
                     WLHLTNNWNAVCLAGTLGAALAIEPSKDERAWYAAAAEKYIQLFLSGFGTDGYCYEGI
                     GYWNYGFGHFIILTEEIRKATQGRIDLFKLPEVEKPAQFGSRDEIVKGIYPTISDVNP
                     GAKPDPQWIRYVNERFQISPEPGAKYKFSVGTQNLGEAVFTLFAEEPWPVAKTVASGN
                     VSLLRTWFDKGGVLISRSGTDEAKGFAVVLKGGSNGESHNHNDVGSFSVVRGRAMIIC
                     DPGGENYTRRTFGPHRYDSGVLNSFGHSVPVLAGQLQVDGSAARAIVLRSDFSDAKDI
                     LAFDIRSAYHVPELKKLERTFVYHRGPDAGLRVSDEVAFEKPEQFESALITWGDWQEI
                     STNVFKMSDAGEELRVTVDTGGVPYTIRKMLINEDVHTRKKPWHIGIVLDRPVKEAKV
                     SMDIRPVFGKN"
BASE COUNT         2601 a         3191 c         3070 g         2694 t
ORIGIN      
        1 gtcgccagtg ttgtccaccg agaaggccac cgtgcccagt ccggacagaa tcgtgaaagt
       61 gccgccatta tagatgccaa agcgccccaa gttgtccacg cccagcgaaa ccttgctgcc
      121 ggtcaggtca ttatgaatga ggtccagctt gaattttacg ccggttccag tgaaggagga
      181 accaatggca aacaggcaat ccatcgtcca ggtcggaccg ggactggaaa tccactgata
      241 aacatattga ctgcttgccg cgtcaaagcg caaggcgttg ttggtcccgt aaaaattgga
      301 agatagatgg atgctcgtgt atttgggact gcattgccag tcgcgaaaca ggcattcata
      361 atccttgagg ccgaccgaaa tcgatgtcag cagcaatccg cgccggttga aatcggcgtt
      421 ccgaaagact ggcggcggcc ccaggggcgt tccgctggca tcgggcacat tgtcgagttt
      481 gattgtgccc tgccagccgc cgtttgtctg aatactatcc cacgtgtagg cataatccat
      541 atgatatgcc acccgtgagt tgtaggtatt gccaaacatt ttgatgccgg aggcgatgag
      601 gcccatgttc gtttgcagaa acgattgctg gaagctggtg gtcccgctgc tcaactggct
      661 ggcctgcgag gaggtcaagt cgcaatcgta actctgggga gaatatccgt tgtcgctgaa
      721 gtgcaaggcg gcgtagggcc ctgatccgcc cgaagcctgg taggtgaacg aattgctgct
      781 gacttggaaa taggtcccaa aaaattcatc gccggcggaa tcagcgttcg tctcattata
      841 caattctgcg ctgacgaatg gccacgccga cgcgctcgtc gtgctgatga agttgcaatt
      901 tttcactacg cagtattcgg aatcatcccg aaagcgcaca taatctgcca gacagtcctg
      961 aaaaacgcag tttgacacca acacgttctc gatatcatgc ggtgcataaa tcatatgttg
     1021 gtggctgccg gcggtgatat tcgtgaaatt acaattaagg acctggatat ctctcgttcc
     1081 attgagcagt ccaatggccg cgtaataggc attcgtcagg ttcaagaacc agcaattgga
     1141 gatttcgatg tttcggcacg gattggagcc attcggtatg caatagattc cccaatacgg
     1201 tgcgggaccg gtaaggacca ggccattgac ttgaaaattt tgacatccgt atagctggag
     1261 gatattggca gaggggctga acacggggcc gtgaggattc accgctgcca gtgtcaacgg
     1321 atacaacgga ttgcccatat tcgttaaact caggctgccg gcattgtatg cgccgtcttg
     1381 gaagttgacg gtgacatttc cggattgaag ctggctttga acggtgctcc agaaggagat
     1441 gttgagataa tacgccgcgt tcgcaaccga agagccgtta cccgtcccgg cagccgtggg
     1501 ggccacccag tagttggtgc ttgcggccac ctgtgctacc gcaagcgccc aggagcaaga
     1561 cacacacaac aaaaagacca ggcttctaaa aactgatttg cctatgcgat tgattggtgg
     1621 ccccgatttc atcacgcccg tgagattgga cgccgctcgg aaccggtcgc atggagatat
     1681 tttacaactg catcagtccg gcagttgacg tgaagcttct cgtaaatccg gcggatgtaa
     1741 gtgcggattg ttcccatgct caaattcatt tccccagcga tttgtttgta agcgcgcccc
     1801 ttggaaagca gttcgagcac ttgctgctcg cgaggtgcaa gctgctcgat caatggaacc
     1861 gcacaaacgg ccggttggaa agcttgcacg agttttcttg cgatgtgact ggacatcgga
     1921 gaaccgcctt tcgcgatatc ctcaattgcc tcgagcagtt tttccggtgg cgtatttttt
     1981 accagataac cgcatgcgcc ggcggcaagg gcttcaaaaa tccgatcgct gttctcatag
     2041 actgtaagca ttatgatttg caggcccggg agcttttcct tcaagtgctt tacgcattca
     2101 atgccgctca ttttcggcag atttatatcc atcaaaacca cagccggagg acgattcggg
     2161 acatctgcca gggcttcctc agccgaggca tatgcgccca gacactgaaa tcccggggcg
     2221 ccttgaataa agcggcaaat gctttcgcac aacccggcgt catcttccac tatactgact
     2281 gcaatagcca taaatcgggc ttaaaaatac tccggatcgg catccgcttc caatcggata
     2341 tatggatgac atgccgcagg gcattcctat tgaggcacgg agttgagcgg aacaatgagg
     2401 cgaacgcgcg tgccttgtcc cggcaggctt tccacttcgc atcgcccatt gatcgtttca
     2461 agccgctcgc gaagattgct caggcctcga ccggtccggc gagtcctgcc attggattcg
     2521 gcgggatggg gttgattaat cccatgccca ttatcggcaa tgaccacttc aagttcatgg
     2581 tcttttgtct tcatgctgac ctgcacttgc gttcctccag agtgtttcag cgcattattc
     2641 aacgattctt tgacggcgag gaaaatattg tggcggacat cggtcagtaa tttataatca
     2701 ggcagtttgg ctgggattat aaggtcgcac gacagcggtc catcagcgca aaattcactc
     2761 gcataacgtg agatgtaatt cgcaaggttt tcaagcgagt catttcctgg atcgaccgcc
     2821 catacgattt catcgaggga tgcgaccaaa tcgcgcgcgg tttcagccat gctgcccacc
     2881 tgccgttccg cttcggtccg atcatccagg ctgcgcaacg ccatcgcacc ttgaaacgag
     2941 atcctggtga gcttcgcgcc aagttcgtcg tgcatgtcac gggcgattcg ggtgcgctcc
     3001 ctttcaatcg cgtgctggta ttccaaacgt tccaaccggc gctgcatgcg acgccgcgta
     3061 gcatatcgcg tggcgatgcc tatcaaggcg atggccagta aacttgacaa acccaaaaac
     3121 caccacgttt gccaaaactg cgggcggagg accaatgcga ccgtcgcgcc agattcgttc
     3181 caaatgcctt cgttgttgca ggcgatgacc tggaaaacat agcttcccgg tggaagccgc
     3241 ttatatgcga cagtccgccg accctctgcg tccacccaat cagaatcaac tccactcaat
     3301 ttatatttga atcgattttt ttcaggagct tgcaggctta acgccgtgaa ttggatttcc
     3361 aattctccgt gcgacggcgg aatttttaat ttaatgggca aggattttgc cggcaaccca
     3421 cccgtcattg cattggtgac atggattgat gatgacgaat aaacggggat gccgtcggcc
     3481 agcacggttt caatcaccac gggcgggggc aatcgatttg gaactatcct tgccgggtcg
     3541 gtcacaacaa ctccctgcgt ggtgcgaaac cagaaacggc catccgaatc cttgcacgcc
     3601 gccggctggg tggtctcttg cgcctgcccg gttcccaaaa caccctcagc cttgccgtaa
     3661 ctgatgcagt taagcctgtt tattttgcca tcagcaagtt ccgcaaattg attttttgcc
     3721 acgcggaaaa tacctttccc gctgttcatc cacaaattgt gctcgccatc ttccatgatc
     3781 gcatagatgg aatcggcggc caggccgcga cgagcagaaa taacggaaaa attgctgctg
     3841 gttaatcgcg ccagcccgtc cgcggctgtg ccgatccaca acacgtgttc cacgtcttcg
     3901 tagatcgaaa ggacacgctt tgtaaaatct gcgcctggaa ttgtgccctt ttcaacgact
     3961 gttccgttcc atcggcgcag gccggtgtca gtgcctatcc aaaggttgcc gacgtggtct
     4021 tcgcagaggg cattgactgc gcagttgctt aattcaatcg aacctccatt tgcaagctga
     4081 tcttctcgct cgaaccgtga taaacccatg tgtgaaccaa tccacattgc gcctttacga
     4141 tcttcgagta aacaagtcag cgacgaaccc aatcccgaca cctccgcatt ctcgttaaga
     4201 attttgtcat ctttgaaacg attcagaccg ggagaatagg cccctgcgga gccggatgca
     4261 taggcagtgc ccacccagag accggaatca aaatcacctg gcatgagcgc cacgacaaag
     4321 tccgaagaaa gcccattgct tcgcgcataa gccgtgatga tgccatcttt gaggcgatcc
     4381 aatccgccac cccacgtgcc gatccacacg cttccatctc gagtcgcgca aacggccgtg
     4441 gtggcattct gtgtcaggcc agattgtttt gtgtatgcag taaaaggctt ggtggtcaat
     4501 cgcgtcaggc cagtttctga cccgacccaa atgtcccctt cccgatcttc aaataatgaa
     4561 taaacccggt atgaagttcc ttccggggcc tggttctcat aaaaatttgc gtcgatgaac
     4621 cggcttaaac caccttgtgt gcccacccaa agatttcccc catgatcgcg cagcagcgcc
     4681 gttacgattc ccggcggccc atttcccttc gaatagtggg tgaaagtccc atcgcgaagg
     4741 ctggtcagcc cgcgattatt cccgatccaa atcgttccgt cctgatcttc gtacaaagcc
     4801 cggatcgcgc gcagcaccag attatttgcg accttgtatg tgagagcttt ttcatctttc
     4861 aagtattgca cgcttctggt cgcaccgatc cataaccccc cattacggtc aggatagagt
     4921 gaaagaagca cattggttgc cgatccgaaa ctttcctcga aatgaagtct tccttgtcgt
     4981 atttgtgcga ggccgtcatg gaatgcgacc catatttcgt tcgtgccgga ttgcttgatt
     5041 tgcacaacgc catcgttggt caatgccgtt tcataatgcg cgagcgcgtc tcccttataa
     5101 caatacaaac cgtgctgctc agtgccgatc cacaacgaac catcgtccga ggcacaaagg
     5161 gcgttgatgg ttggttgcgg ggaactgcgg gtaaccatca acggcgtgaa cttgattcca
     5221 tcgaaacggg ccaatccttc ccgagtgccg acccacaaat agccatcttg cgtttgggca
     5281 attgacaaaa cgttgttgtt tggaagccct tcctcggttt gccaaagcgt gggattgaat
     5341 tcggtggcag gcagagcgat caatggctgg catatcgcca aattcaggag aaacagcaat
     5401 aatacccgcg aggcacgctc caaagaaggt gtaacccatc tcgatgctct gttcttcgag
     5461 tgcattcaga caacgtcaac tgggtgggac gtcatccttt tatagcaccc ctctgaatgg
     5521 acgagaagcc tttttagccg taatcaggcg gtaggatcaa cgagagcgcg cctttgccag
     5581 tccggaagga accctggctc gccacccatt tccttttgca ccattccggc cgggcattaa
     5641 attgcattgc gcgttggaaa agccgcaacc tccgttacat cattgagttg gaaaagcgcc
     5701 agttctgcgc tcgctaattc acgccactgt tgtaagcgtg atgaaacgtc cagatgatat
     5761 cttggctgtc gttcggatat acgacggttc catacaaatc tccattataa gtaactggta
     5821 taatggttga gtgactgagc catttgtgaa tctcggcatg accgtcggcg aacgaaaacc
     5881 cgcaatactt tcctttatga taagaggcgg gcatgtcgtt ccagttacaa ttgccgtttt
     5941 gcgtgacacc tcccggagga aaagaaaaga caccgtcgta tggatggctg gcgccgccgg
     6001 catcgcctgt atcaggatgg gcatctataa agacccagag cgaggacggg ctcgtcatgt
     6061 cgctcatttt ccaattctgt gtaaaattgg gcccccagct tgttgaatcc caagtgggtt
     6121 ccaccggttt atgcgccaga tatccgttga ctcgcacgct ccgaatacgg ccagaccatc
     6181 cggccgctcg ttgggcagga gacaaataaa tatcggccgg gcacaggtaa cagctgatgc
     6241 tttgggaagt ataacttccc agcaggcccg cgcttaaatt ggcgacgttg gtattgggac
     6301 tacttgcccc tgccgtccag tccagataac tggtcaccca tccgccggtc gaattgccga
     6361 cctcgtttat tggcaattgg tcggcattgt cccccacata catattataa gccaactgga
     6421 tctggcgctt attattgatg caaccagtcc ccttcgcact ctgtttggcc gccgacaagg
     6481 ccggcaacag cagcgccgcc aaaatggcaa tgatggcgat gacgaccaac agctcgatca
     6541 gggtgaacgc gtcggcggcc tcatcagtga tggccaatcg ttttttcatc aatatgccag
     6601 gaaggagctt aattcgactt gataatgaag aactgcgcag ggacgctgtt cgttacggcg
     6661 ttggtgacgg caaaattgcc gctcgcgttg taggacccag tggagaaggt cgtccaattc
     6721 gtcaggggtg cggccacatt ggtggaggta agcactttga aaccatccaa tggatagcca
     6781 ttggttcccg cgaagacgat gttgctgccg ctgaatgaaa aaccggtgat gaccgggatg
     6841 ggcaatctgg cgacggaaat ggagccatcc accgcaagat tattgttcca aatcactttg
     6901 ccgcccgaaa ccgccatggc attgccggtg agaggttcgc tgaagatttg gaacgtgtcg
     6961 cccacgacga gcgccgggcc aaggttagta accgttagtg ttcccgagcc ggaattggcc
     7021 aaggtgccgg taacagccac cgcgccattg cttggtgaaa gagatttatt gatggcaatg
     7081 gctgcgttgc cggcaatggt gaggttgttg cttatcgtta atgtacccac cgacgatgcc
     7141 gctggtcccg gagcaagcgt cgcacccgaa ttgatcgtta ccgcgccgct gataaagcca
     7201 cttccgccaa gcgtggcgcc cgagctgatg accacagaac cagtgcctgt gccgctcttg
     7261 ccggtgttgt tgatgagcaa tgtgcccccg ctgttggtgg tgttgccggt gtaagtattt
     7321 gcgccggtga gcgtgacggt tccgctcgcg gtcttggtca cccacccgct gccgctgatg
     7381 acgccgccat atgaatagct gcccgtgcga ttaaatgtca gcgtgccgtt gtcagtggta
     7441 ttgcccgtgc caagcgttcc ggtcgtcccg cctgcaccga cggtcaccag gctgctggca
     7501 ccgacagtag tagttccggt ataggtattg ttggccgcca aggtcacagc cgaggtgacg
     7561 ccggcatgcc cattgggaaa attcatgctt cccggcccgc ttatcggcga gttaatagtc
     7621 acagttccgc cagacgacac gacatcttcg atcgttccac cgccggacgc aacgtagatt
     7681 ccgcgcgtgg cgccgatcgt ggtgctgtgg cctacatgca tcgagccgcc attgaaaatg
     7741 atgttggacg cgttaagggt gctgggcaac gttcccaaac ttgaaacttg accggagctc
     7801 tgatcgtaat tgaaagtgag actgcccccg ttcaccacca ggttgctgta gatttccagc
     7861 gaaggattgg cgtatcccat attgcaatcg ccgctgccgc tcttggtgag ggttcctgtg
     7921 ccaagattga ttgtccctgc gcttgcgcca gccgcaccgc ccggtctcat gctccccgag
     7981 ccgccgagcg tgacagagcc atttgattgg ctatatatat tgcaagtcgt ccaagtcccg
     8041 ctattgacgg tcaacgtgcc gccattggtg gtcacggtaa taccgtcatt ggcccacatg
     8101 gaataggagc cagtcgagat gattgcgctg ccgtccaaaa gcacgtttgt cgtcatcagg
     8161 gaggctgggt tcacgttcgc tccaaagctg ttgttgtttt cgacgtaaaa cgtggcgcct
     8221 ggaccaacat acgtgctttt ccaaaccgtc ggcgcaatcg agttcaggta agcgtaacac
     8281 gacgcggggc cttcgacgat cagggtaccg ccaatactgg ttgcacccat atcgaagttg
     8341 cccgtccccg atgcgctgat aatgagatta taattatttc cattatcggt aagcggcccg
     8401 ttttccccgt cgtaaaccaa agcggcgccg gtatccacat aaatcatact gtcgttctgc
     8461 cccaatatga tttgtccata aaaagtggtc acacccgaga ggttcctaag cgccccgctg
     8521 ttggtgatac ctgaaccatt gatggttacg ctagaagtaa atgacgttaa tccgttcgtg
     8581 ctgccgtttg cgtcgccgtt caataccaaa gcagcgccat tcgacaccgt gaccggagca
     8641 ccctgctggc cgaccgcata attgttctca accgaaatat cgccctgatt gataaccaca
     8701 ctgccggtga tggtcgaggc gttgttcgtg gtcaaatgca gccgtccgag accgttcttg
     8761 gtcaaaatgg tcgaattggc gattggcgcg gcgatggtat gttccccgcc accggtatca
     8821 atcgttccat tgagcagagt cagcacttcc gcgccggtgg cgttcgtcag tacatagctc
     8881 cctgtagtgt ttgtaaaata aaggtctccg gcggtgatgt ttgtctgaag cgtggctgtc
     8941 ccggctgtcc cggtaaaaaa ggcatcgtcg ccattggcat tcatccaaat attgtcttga
     9001 gtgccatcat accaaaaagt accaaccgag tcccaatttc ccgtgccacc gagcgcgctt
     9061 ccggtggtcg cattggggtc ccaataaagc gaggctgctt gaagagaaaa aacggcaaag
     9121 aatatcgtgg cgctaagggc tgttgctaaa atgtgtttca tgcacgttag agatttgatt
     9181 tcgtcgaagt ttaaacacaa gtcgccagag aaaatagtca tacgataata tgacatggac
     9241 ggaaggaggg tcctgccgct ttgaagattg cctttaggaa ggtacagcat ggccatcaag
     9301 cactaaacca aaaatgattt tgcaacggac tcgccggggg gaccgacagc ctggagcccc
     9361 gacccttccg ttggcattcg atccgaaccg gaggcggcag cagtctctac ggatttgatc
     9421 gaattacatg ccgcttgtgg acttatcgcc accgcatgcg gaggaaggaa cgaactttcc
     9481 gggctgttaa atctccgttg caccttccct tgcctcgaca attccttcgt ttaatttttt
     9541 ccgaacaccg gcctgatgtc catgctcacc ttcgcctctt tcacggggcg atccaaaacg
     9601 atgccgatat gccacggttt ctttcgggta tgcacgtctt cgttgatgag cattttgcgg
     9661 attgtgtatg gcactccacc cgtatccacg gttacccgca attcctcgcc ggcgtcgctc
     9721 atcttgaata cattcgtcga aatttcctgc cagtcgcccc aggtgatgag ggctgactcg
     9781 aactgttccg gcttttcaaa cgcaacttcg tcgctcacgc gcagaccggc gtccggaccg
     9841 cgatgataaa cgaaggtgcg ctccagtttc ttcaattccg gcacgtgata cgcggagcgg
     9901 atgtcgaacg ccagaatgtc cttggcgtcg ctgaaatcgg agcgcagcac aatcgcccgc
     9961 gccgcgctgc cgtccacctg caattgtccc gccaagaccg ggacggagtg gccgaaggag
    10021 ttcagcacgc cgctgtcgta acgatgcggg ccgaaagtcc gccgcgtgta attttccccg
    10081 cccggatcgc agatgatcat cgcgcggccc cgcacgacgc taaagctgcc aacatcgttg
    10141 tgattatggc tctcgccatt gctgccgccc ttcagaacga ctgcgaaacc cttcgcttca
    10201 tctgtgccgg aacgcgagat gaggacgcct cctttgtcaa accaggtgcg caacaaagac
    10261 acgttcccgc tcgcgacagt ttttgcgaca ggccatggtt cttcggcgaa cagcgtgaag
    10321 acggcttcgc ccagattttg cgtcccgacc gaaaatttgt atttcgcgcc gggctcggga
    10381 gagatttgga agcgctcgtt gacataacgg atccattgcg gatccggttt cgcgcccgga
    10441 ttcacatcgg agatcgtggg atagattccc ttcacgatct cgtcgcgcga accgaattgc
    10501 gccggcttct ccacttccgg caacttgaac aagtcaattc tgccctgcgt ggccttccgg
    10561 atttcctcgg tcaaaatgat gaaatggcca aagccgtagt tccaataacc gatgccttcg
    10621 tagcaataac catccgttcc aaaaccgctc aggaacaact ggatgtattt ctccgccgcg
    10681 gcggcatacc aggcccgttc atcctttgaa ggttcgatgg ccagcgccgc gccaagtgtt
    10741 ccggccaggc agactgcgtt ccaattgttc gtgagatgga gccagtaggc gtccttgagc
    10801 cggccttcca cggttccgcg aaacggaagc aacacgcgtt tgcggatgtt ctcgcgaagc
    10861 agttgccgcg tggcctgggg cagcttgtcg ccaagcaggc aatcagccgt ggccaggtcg
    10921 aaagcgagcg tggtggcccg cagatcggga cgcatcaact tgccatcaaa aacttcgagt
    10981 ctcaggtcgt gcgcgggata ggtccacgag cgctcggcgc acatggcttc gatgattttt
    11041 tgcagcggct tcaggaaacg ccctcggtcc tcgaaacatt ccgcgagggt gagaacgcac
    11101 agccgcgacg agcgggccaa ctggaccttt tgcccgcgat cgcggtttcc ggtgcgcaaa
    11161 cagtcgagat acagatcatc cgtcatctcg ggaatcggct cctcggccaa tttttccgcc
    11221 tcagggatga tgggacgcac tgccggattg tcagccatct tcgcccagac ggctcggttg
    11281 gtgatgggta cgccgaaact tgccgttaac ggagagagca tcgcagcaat ctgctggacg
    11341 cgctgcgggc tcaccatgtt cgttgcggcg caaaccggca tcgcccacaa aaccatgaag
    11401 gcgaagaggg cgcgaagaag atgtcggaga atgttcatgc gagggaacgc ttcagtgaaa
    11461 actggagatt gctccataca acgagggttt tcatgcggca gttacaagtt gtcactttag
    11521 tctcaggcgc atagttcgac tcgaccactc caagtt
//
LOCUS       DASZMM010000228         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_905695, whole genome shotgun sequence.
ACCESSION   DASZMM010000228 DASZMM010000000
VERSION     DASZMM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905695"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VG754_05400"
     CDS             <1..1939
                     /locus_tag="VG754_05400"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWF18679.1"
                     /translation="SGELQDAILNQLYKRADWTMAFLDAIESKKINLAALGPLAINRL
                     RTHSNADVAARANKLIDALRGPETKEKDALIAKFTPIVTQPGDAVRGKELFAKNCAVC
                     HKFKGAGKDVAPDLTGMGAHGAAELIVHVLDPNRSVEPNYFAYSIETKDGEIYDGVIA
                     RENSASVTLRNAAGDMEIPMANIKTRRNTGLSLMPTGFETLGAEILRDILAYLCEGEA
                     NFRIIDLQSALTASSLKGLWLTEDNLYDTLDFKKFGVVKVGDVPFEIVNPLKSATGKN
                     LILLKGGTGLAKTYPQRVEVTGVNAKAARLHFLGGVAGWGYPLRDTQKEMPVAKVTVH
                     YADNNSEEFIFKNGVEIADWNGATDVPGSKSASELVKHGQVRWFTKELKRPGIIDSLT
                     IESFDNSVSPAFVAITADSSASPVTDSTNNSPLLKEFKWMPGTTHVLILGGGSSHDFQ
                     RWFNLADSATLTALGNVSVNYTENPGDLPSALKDADVLFESSNQPIDSATRKAIFDFA
                     DSGHGLLLVHPGLWYNWNDWPDYNRVLVGGGAHGHDKYGEFEVVAKESSHPVMAGVPV
                     TFKISDELYHSEIDPQGTPVEVLAEARNLATGKTYPSVWIVKHPKARIVCIALGHDAK
                     AHDLDAYKSILQNAVKWAAER"
     gene            2067..>3231
                     /gene="serS"
                     /locus_tag="VG754_05405"
     CDS             2067..>3231
                     /gene="serS"
                     /locus_tag="VG754_05405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWF18680.1"
                     /translation="MLDIKLIREQPDFVRQRLATRGAGDEARIDDVLKWDEARRKTVS
                     EVEVLKAHRNKVSKEIGALMGQKKIAEAEEKKKETRDLGDKITQLDQQAAAAEAARDE
                     VMLRLPNLPHESVAIGKSAEDNPVMREWGKKVEFNFKPKSHIELCESLKLVDFARAAK
                     LSGSGFLLYTNWGAKLERALIQFLLELHTSQHGYTEISPPFMVGPKCLEGVGQFPKFK
                     DQYYGVAEGDDPAKLGKLYLIPTAETPVANIHREEILPEKQLPIFYCAYTPCFRGEAG
                     AAGVGTRGMIRVHQFDKVELIKIVKPQQGYDELEKMVANAEKVLQLLGLHYRVIMLCT
                     GDMGFGSAKTYDIEVWAPGQGQYLEVSSCSNCEDFQARRMSLRFKSENGENKFP"
BASE COUNT          783 a          836 c          934 g          678 t
ORIGIN      
        1 gagcggtgaa ttgcaggatg ccatcttaaa ccagctctac aaacgcgccg attggactat
       61 ggccttcctc gacgcgattg aatccaaaaa aatcaacctc gccgcgcttg ggccgctcgc
      121 gatcaatcgt ctccgcacgc atagcaatgc ggatgtcgcc gcgcgcgcga acaagctcat
      181 tgacgcgttg cgcgggccgg agacgaagga gaaggacgcg ctcatcgcga agttcacgcc
      241 cattgtcacg cagccgggcg atgccgttcg cggcaaggaa ttgttcgcaa aaaattgcgc
      301 ggtttgccac aagttcaaag gcgcgggcaa ggacgttgcg ccagacttga ccggcatggg
      361 cgcgcatggc gcggcggaat tgatcgtcca tgtgctcgac ccgaaccggt cggtggaacc
      421 gaattatttc gcctacagca tcgagaccaa ggacggcgag atttatgacg gcgtcatcgc
      481 gcgcgagaac agcgcgagtg tcactttgcg caacgccgcg ggcgacatgg aaattccgat
      541 ggcgaacatc aagacgcgcc gcaacaccgg gctttctttg atgccgaccg gctttgaaac
      601 gctcggcgcg gaaatattgc gcgacatcct tgcgtatctt tgcgaaggcg aggcgaattt
      661 ccgcatcatt gacctgcaat ccgcgctcac ggcgagttca ctgaaaggac tttggctcac
      721 ggaggataat ctttatgaca cgctcgattt caaaaaattc ggcgtggtga aagtcggcga
      781 cgtgccattt gagattgtca atccgctgaa atctgccacc ggcaaaaatc tgattctgct
      841 caagggcggc acggggctgg cgaagactta tccgcaacgc gtcgaagtta ccggcgtgaa
      901 cgcgaaggcc gcgcgtttgc attttctcgg cggcgttgct ggttggggct acccattgag
      961 ggatacgcaa aaggaaatgc cggttgcgaa ggtcacggtg cattatgccg ataacaattc
     1021 ggaggagttc atttttaaaa atggcgtgga aatcgccgat tggaacggcg cgaccgatgt
     1081 gcccggctcc aagtccgcga gcgaactggt caagcacggg caggtgcgct ggttcacgaa
     1141 agaattgaaa cggcccggca tcatcgactc gctcaccatc gaaagctttg ataacagcgt
     1201 ctcgcccgca tttgtggcca tcaccgcgga ttcgagcgca agtccggtca cagattcaac
     1261 aaacaattcg ccgctgttga aggaatttaa atggatgccc ggcacgacgc atgttctcat
     1321 cctgggcggc ggttcgtcgc acgatttcca gcgttggttc aatctggccg attcagcaac
     1381 gctcaccgcg cttggaaatg tttcggtgaa ttacaccgaa aatcctggcg acctgccgtc
     1441 cgcgctcaag gatgcggacg tgctgttcga atcgagcaac cagccgattg attcggcaac
     1501 gcgcaaggca atttttgatt ttgcggactc gggccacggc cttctgctcg tgcatccggg
     1561 gctttggtat aattggaacg attggccgga ttacaatcga gtgctcgtcg gcggcggcgc
     1621 gcacgggcat gacaaatacg gcgaatttga agtcgttgcg aaggaatcca gtcatcccgt
     1681 gatggctggc gttccggtca cattcaagat ttcggatgag ctttatcact ccgagattga
     1741 tccgcaaggc acgccggtgg aagtcctcgc cgaagcgcgt aatcttgcga ccggcaaaac
     1801 atatccctct gtatggattg tgaaacatcc caaggcacgc attgtctgca tcgcccttgg
     1861 ccacgatgcc aaagcccatg atttggatgc gtataaaagc attcttcaaa acgccgtgaa
     1921 atgggcggcg gagaggtaaa ttggcaaaac ccttgaaaat accttttgct gccaatttaa
     1981 actttccgcc ctcaaccatc aactctcaac catccaccat ttaatccccg cttgcccggc
     2041 tgcaaactga ttcttatagt atcgcgatgc ttgacattaa attgatccgc gaacaaccgg
     2101 atttcgtccg ccagcgtctc gccacgcgcg gggcagggga tgaggcgcgg attgacgatg
     2161 tgctaaaatg ggacgaagcg cggcgcaaaa ccgtttccga agtggaggtg ttgaaagcgc
     2221 atcgaaacaa ggtgtccaag gaaatcggcg cgttgatggg gcagaaaaaa attgcggaag
     2281 cggaggaaaa gaaaaaagag acgcgcgatc tcggcgataa aatcacgcaa ttggaccagc
     2341 aagccgccgc agccgaagcc gcgcgcgatg aagtgatgtt gcgcctgccc aacctgccgc
     2401 acgaatcggt cgccatcggc aagagcgccg aagacaatcc cgtgatgcgc gaatggggca
     2461 agaaagtgga attcaatttc aagccgaaat cgcacatcga actttgtgaa agtttgaagc
     2521 tggttgattt tgcgcgtgcc gcgaaacttt ccggaagcgg ttttctgctc tacacgaact
     2581 ggggcgcgaa gcttgagagg gcgttgatcc aatttttgct cgaattgcac acgagtcagc
     2641 atggttacac cgaaatttca ccgccgttca tggtcggacc gaaatgcctg gaaggtgtgg
     2701 gacaattccc aaagttcaaa gatcaatatt atggtgtggc ggaaggcgac gatcccgcca
     2761 agctcggaaa gctttatttg attccaaccg cggagacgcc ggtggcgaac attcatcgcg
     2821 aggaaatttt gcccgaaaag cagttgccaa ttttttattg cgcatacacg ccttgttttc
     2881 gcggtgaagc gggcgcagcg ggcgtcggga cgcgcgggat gatccgcgtg catcaattcg
     2941 acaaggtgga attgatcaag atcgtgaaac cccagcaggg ttatgatgaa ctggaaaaaa
     3001 tggtcgcgaa cgcggagaaa gttttgcaac tgctcggctt gcattatcgc gttataatgc
     3061 tttgcaccgg tgacatgggt tttggcagcg cgaaaacgta tgacatcgaa gtctgggcgc
     3121 cggggcaggg gcagtatctt gaagtgtcga gctgttcgaa ttgcgaggat ttccaggcgc
     3181 gccggatgag cctgcggttc aaatccgaga acggcgaaaa taaatttccg c
//
LOCUS       DASZMM010000229         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3392890, whole genome shotgun sequence.
ACCESSION   DASZMM010000229 DASZMM010000000
VERSION     DASZMM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3392890"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VG754_05410"
     CDS             complement(<1..455)
                     /locus_tag="VG754_05410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18681.1"
                     /translation="MSELRIRAGPLERLVYDQQLNSTVSVKLMDYKVLGEHVRDTYRE
                     SAAQYRQDDEIEIATDVHRRLTGILANLTSSYNHPITVLDVGCGTGRYFHCLKNVRRL
                     KGIDVCDEMLRIAQNPVRQEEITVNEIQLSCENVFLAKFPPGSFDFIYSL"
     gene            complement(464..955)
                     /locus_tag="VG754_05415"
     CDS             complement(464..955)
                     /locus_tag="VG754_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWF18682.1"
                     /translation="MQKLNSLDNLFLLEVQDLYNAEKQITRALPKVAKKVSSPKLKSA
                     IEEHLQQTETHVNRLEEVFKILGQPTKGHACAAMKGILAEADELLGQKAEPAVLDAAI
                     IGAAQKVEHYEIASYGSVIQWAKQMGRDDIKQLLGQTLDEEEKTDQKLSELAKSDINA
                     RAI"
     gene            complement(993..1880)
                     /locus_tag="VG754_05420"
     CDS             complement(993..1880)
                     /locus_tag="VG754_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWF18683.1"
                     /translation="MKYIGSVSSFILACLFAMVATTFLYGQPERLPGLAPKADAPSKK
                     LRHFTTFVGIRTARELIGKPVEERTGDTIGRVNDVVVNPRTGRIVFIVLSSGGFLGVG
                     AHLKPVPPQALSLAAAKRGILALDISPKRWKSAPRMNKEWLGNLENINVAEKVYAFYH
                     EPWPSVDGTTFTNHADDPVESPAGHLKLASELIGESVIAPRKGEIGRVSDLTVDLKNP
                     AMARAIIRPTDSEHVPFTVPINAFEGDDAGNALFLNRDARALQNNRTRDAGLQPGKMA
                     EVFSPIIFYFDVRSMSFSP"
     gene            complement(1969..2955)
                     /locus_tag="VG754_05425"
     CDS             complement(1969..2955)
                     /locus_tag="VG754_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HWF18684.1"
                     /translation="MSHHSFAPTFHHSSVRLYSAIAQKPKEFGPSLPGIDFERNRAQA
                     CPCSVSLTLLNRNDAIKPGQANREHSPFVTVNGRRSIRHLLEPLAALEGIEAEMLQFE
                     SDGEQHGIPRFVFRGPNSSDPIRLGIFAAIHGDEPAGALALVEFLRDLQAQPALAENY
                     NIWAYPVCNPTGFEDNTRASRQGRDLNRQFWKSSKEAEVQLLEHELCTRHLHGIIQLH
                     ADDTSDGIYGFVRGATLTDELLRPALEAAAKVLPLNRSPLIDGFAAQDGIIYDQYEGV
                     LAAPTEVTPIPFEIVFETPQLAPLELQAKAFDIFLHTVLAEYRRLISFAQNI"
     gene            complement(4118..>4315)
                     /locus_tag="VG754_05430"
     CDS             complement(4118..>4315)
                     /locus_tag="VG754_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18685.1"
                     /translation="SKIKSLQGMLPICGFCKGIRDDEGAWIKLEDYIRAHTEATFSHG
                     ICRACSEKHYPQLFTDKDKAA"
BASE COUNT          969 a         1141 c         1175 g         1030 t
ORIGIN      
        1 agcgaataaa tgaaatcgaa agagcccggt ggaaatttcg cgaggaaaac gttttcacag
       61 ctcagttgga tttcattgac ggtgatttcc tcctgtcgca ccgggttctg cgcgatccgc
      121 agcatttcgt cgcagacatc aatccctttc aagcgtcgga catttttaag gcaatgaaaa
      181 taacggccgg tgccgcaacc gacatccaac accgttatcg gatgattgta tgaagaggtc
      241 aaattcgcca atatgccggt caaacgacga tgaacatccg tggcgatttc aatctcatcg
      301 tcctgccggt attgggcggc cgactctcga taggtgtcgc gaacatgctc gccgagaact
      361 ttgtagtcca tcaatttcac gctgacagtt gaatttaatt gctggtcgta aaccaatcgc
      421 tctaatggtc cggcccggat tcggagctcg ctcatggtgt tcattaaatt gctcgggcat
      481 tgatgtccga cttggcgagt tcggaaagct tttggtctgt tttttcctcc tcgtcgaggg
      541 tctgtccgag caattgcttg atgtcgtcgc ggcccatctg cttcgcccat tgaatgaccg
      601 agccgtagct ggcgatttcg taatgctcca ctttttgcgc cgcgccgatg atggccgcat
      661 caagcaccgc cggttcggcc ttctggccga gcaattcatc cgcttcggcg agaatgcctt
      721 tcatcgcggc gcaggcgtgc cccttggtgg gttggccgag aatcttgaaa acttcttcaa
      781 gccggttgac gtgcgtttcg gtctgttgca gatgttcttc gatcgccgac ttcaatttcg
      841 gcgaggaaac tttttttgcc actttgggca gcgcccgcgt gatttgcttt tcagcattat
      901 aaaggtcctg cacttcgagc aggaacagat tgtccagaga attcaatttt tgcataagac
      961 aaacgggttg tggtttaatt tcgaatggat gtttaagggc tgaacgacat ggatcggaca
     1021 tcgaagtaaa aaatgatcgg cgaaaaaacc tccgccatct tgccaggctg aaggcccgca
     1081 tcgcgcgtcc ggttattttg taatgcccgc gcatcccgat tcaggaacaa ggcgttgccg
     1141 gcgtcatcgc cttcgaaggc attgattggg actgtaaacg gaacatgttc cgaatctgtt
     1201 ggcctgataa ttgcgcgcgc cattgcggga ttcttcaaat ccaccgtcaa atccgaaacg
     1261 cgtccgattt ctcccttgcg gggtgctatg acgctttcgc caatcaactc actcgcaagt
     1321 ttcagatgcc cggctggcga ttcaacagga tcatccgcat ggtttgtaaa agtagtccca
     1381 tcaactgatg gccaaggttc gtgataaaag gcataaactt tctcggctac attgatattt
     1441 tccaagttgc caagccattc cttgttcatg cgcggagcgg atttccacct tttggggctg
     1501 atgtccaacg ccagaatgcc gcgcttggcc gcggccaggg acaacgcttg cggaggcacc
     1561 ggcttcaaat gcgctcccac gccgaggaac ccgccgctcg acagcacgat gaaaacgatg
     1621 cgtccagtcc gcggatttac gacaacgtca tttacccggc cgatcgtgtc gccggttcgc
     1681 tcttccacag gcttgccaat caattcgcgg gcggtgcgaa ttccgacgaa tgtcgtgaaa
     1741 tgccgcaact ttttggaagg agcatccgcc tttggggcca gaccgggcaa tcgttccggc
     1801 tgaccataca aaaacgtggt cgccaccatc gcaaagaggc aggcgagaat gaaagacgaa
     1861 acgctcccaa tgtatttcac attcagagca tccttcagtc gcatttgaat ctccatgggg
     1921 tattctcccg aacccaaaca tcattggaag cggttgcggg acttatgttt agatattctg
     1981 cgcgaacgaa atcaagcgcc ggtattccgc caataccgtg tgcaaaaaaa tgtcgaaggc
     2041 tttggcctgc agctccaatg gcgcaagttg cggcgtttca aaaactatct cgaaaggaat
     2101 tggcgtgact tccgtcgggg cggccaagac tccctcatac tggtcataaa tgattccgtc
     2161 ctgtgccgca aaaccgtcaa tcaacggact tcggttgagc gggagaactt ttgcggcggc
     2221 ttccaacgcc ggacggagca attcgtcggt caacgtcgcg ccgcggacaa atccataaat
     2281 cccgtcgctc gtgtcgtcgg catgaagctg gatgattccg tgcaaatgcc gcgtgcacag
     2341 ctcatgttcc aacaattgca cctcggcttc cttcgacgac ttccagaatt gccggttcaa
     2401 atcgcggccc tgacgcgaag cgcgggtgtt gtcctcgaaa ccagtcggat tgcaaaccgg
     2461 ataagcccag atgttgtaat tttccgcaag cgccggttgt gcctggagat cgcgcaaaaa
     2521 ttccaccaac gccagcgctc ccgccggttc gtcgccatgg atcgccgcaa aaattcccaa
     2581 acggatcggg tcgctgctgt tcggcccgcg aaagacgaaa cgcggtatgc cgtgttgttc
     2641 gccgtctgat tcgaactgca acatctccgc ctcgattcct tcgagcgcgg cgagcggttc
     2701 cagcagatga cgtatggaac gccggccatt caccgtcacg aacggagaat gctcgcgatt
     2761 ggcttgtccc ggcttgatgg cgtcgtttcg gttcaacagc gttaaactca cagaacaggg
     2821 acaagcttgc gcgcggttgc gctcaaaatc aattcccggc agagatgggc cgaattcttt
     2881 tggtttctga gcgattgcgg aataaaggcg aaccgaactg tgatggaatg tgggagcgaa
     2941 ggaatgatgg ctcatcaagg attggaatga gggagcaacg gcggataagc agaaggcttg
     3001 attccacaaa ataatggctt gactggcctt gactcggaat ttgccgaaaa cggagtaatg
     3061 cattgatgga gaattagttg gtgtcatttt cgcgtggctt gaccaaggct tgactcgcgc
     3121 agcttgcctt gaccaaaagg cggcgggttt gacaccaagc gacacgaagc ggcatctggc
     3181 ccaattggga aaacatcgaa caacgaacgc tgaacgctga acatcgaatg gtcaaaccgg
     3241 cgcttcgggt cacattcaac gttcgacgtt ggaggttggg cgttcgatgt ttgaggaccc
     3301 tcccccctgt gtttttggcg ggttaaagcg ggtgtaagtg gttctaagcg ggatttcaag
     3361 tacttacgca tgggatggcg acgcggccca attcggaaaa catcgaacaa ccaacgctga
     3421 acgctgaaca tcgaatgttc aaactggcgc ttcgggtcac attcagttgt tcgaggttgg
     3481 aggttggacg ttcgaagacc ctccccccgg tattttcggc gggttaaagc gggtgtaagt
     3541 ggttctaagc gggatttcaa gtacttacaa cttggcggct caatccgcgc tattggatgc
     3601 caaagctcgg accaagctga agcgcccgca ttgttggcgg ccttggacaa aagaaattca
     3661 ttccagcaac ggaattgccg ggattcgccg acaccggatg gtgccgtttc tcgcgctcct
     3721 tgactcacgc gaccggcagt ctgtcatgac ccggaaggat tgtccattta tttcgggaaa
     3781 agagatgtcg cgcgaaggcc gcgaagtggg cgaaggaaag gcggttacca tttggcgagt
     3841 gctttttgaa ttgccgaaat ggctgttctc cctcccatcc gtgtttcatc cgtgttcaat
     3901 ccgtggctca actccatcgt tccggctcag ccggaatcat gccgatgaaa aaggggagcg
     3961 cacgcgtctc gcgtgctgtt cgagccgtcc cggcgaggac ttttagtttc tggtgcactg
     4021 tttggcggac agaaaatcag tgttacgaag tgttcggcga gacgccgaac actgcacgcc
     4081 ggaggcgcgc gctcccattt caactgcatc gttccggtta agccgccttg tctttgtccg
     4141 tgaaaagctg cgggtaatgt ttttcgctgc aagcccggca gatgccgtgg ctgaatgtgg
     4201 cctcggtatg cgcgcggatg taatcttcca gcttgatcca tgccccttcg tcatcgcgga
     4261 tgcccttgca aaaaccgcaa atcggcaaca tgccttgcaa ggactttatc ttcga
//
LOCUS       DASZMM010000230         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4481632, whole genome shotgun sequence.
ACCESSION   DASZMM010000230 DASZMM010000000
VERSION     DASZMM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4481632"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..628)
                     /locus_tag="VG754_05435"
     CDS             complement(227..628)
                     /locus_tag="VG754_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18686.1"
                     /translation="MKTKFFGALLMASLVGTTGFVTGCVETVDGHSQAGVPFMKDKVE
                     GTYDRTVPQVLNAARAVIKLNGQLTVDNSVNNSLEGKVNQVTVWVKVDEVDPAKPLTR
                     VQVETRNRAGVSDIELAHELEKRIALQLVSQ"
     gene            complement(951..1298)
                     /locus_tag="VG754_05440"
     CDS             complement(951..1298)
                     /locus_tag="VG754_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWF18687.1"
                     /translation="MKITRIIPTLVTITALAGLLAACESESDLKSKAKISEAEARQIA
                     MSKVPNGTIKEAELEKEKGHLIWSFDMAIPDSKDIKEVNVDAITGEVIAVETETPKQQ
                     AKEKDEDEKEGKK"
     gene            1831..>3261
                     /locus_tag="VG754_05445"
     CDS             1831..>3261
                     /locus_tag="VG754_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF18688.1"
                     /translation="MSSKLHILIVEDSEDDAIFVVRELQRGGFQPVVKRVDTAPDFEA
                     ALAAGGWDLIIADYNLPQFSAVDALETLQKSGQDLPFLIVSGAIGEDVAVGVMKCGAH
                     DYILKNNLTRLVPAVQRELRDALARRERRQAQQEYLRMAAIVESSGDAIIGKTLDGTI
                     TSWNIGAERLFGHRAEEAIGRSIAVLVPSDRTEEEERILERLRRGGIVDHFETKRVCK
                     DGRVIDVSITSSPIRDKAGNIVGASKIARDISERVRAQNYLAALSTLGQSLATVSSPE
                     DSARVIGNIADELFGWDAFSLDLYFTDQDQIQTVLNVDTIDGEKRDVPCTYIGNAPSG
                     VARRIIDSGAELILRDDPTATLPDAIPLGNVERPSASLMFVPIRNQTRVIGVMSVQSY
                     RPKAYDARALDTLQTLADYCGGALERIKARQELEISREQLRALAAHLQSIREEERKLI
                     AREIHDELGQALTGFKMDLAWMRNRMQ"
BASE COUNT          722 a          899 c          875 g          765 t
ORIGIN      
        1 cggcgcatag acattttgaa gctcgaagcg cgggcaaagg aaaaaactcg cgccgtgcag
       61 ctcggagaat acgaacagga tattgggctt aagaagggga cgaatcgttg aatcatggag
      121 cgcagctgtg cgtggatcgc cagccgcagc aagttcatca aaatcctgag cgttccacgc
      181 tgctgcggct ggtcctgacg gacacagccg cgttccatga ctgcccttac tggctcacta
      241 actggagtgc gattcgtttt tcgagttcgt gggcgagttc aatgtcgctc actccggctc
      301 ggttgcgggt ttccacctgc acgcgggtga gcggcttggc cggatcaacc tcatcaactt
      361 tgacccagac cgtcacctgg ttgaccttgc cttcgaggga attgttcacg gaattatcga
      421 cagtcaactg gccgttgagc ttgatgactg cccgcgcggc gttaagcact tggggcacgg
      481 ttcgatcgta ggtcccttcg accttgtcct tcatgaaagg cacaccggct tgcgagtggc
      541 catccaccgt ttcgacacaa ccggtcacaa atccggtggt tccaaccaag gaagccatca
      601 acagcgcgcc aaaaaatttt gttttcatgc aaggctatgc aatcacaacc gttcctttcg
      661 tcaagcatga aggaatggaa attgttaatt cgttaaaagg ttacagagcc atcccgtggg
      721 aaaataggat taggaaatca ggaaagcagg aaaagacgat gtttcctcaa gaattttgtt
      781 cctgatttca tgatttccta ataaaaaact cttgatctca ttttctgccg actggcttga
      841 cgccaactat gggatgctgc tgaaaatgtc acatggtcac aagggcgccg ttgcgccgat
      901 gtaacaatgt aactatttaa caatgtaact tttttaagga ggccctgcgc ctatttcttg
      961 ccttccttct cgtcctcgtc tttttccttc gcctgctgct ttggcgtttc ggtttccacc
     1021 gcgatcacct cgccggtgat ggcatcgaca ttgacttcct tgatgtcctt ggaatccgga
     1081 atggccatgt cgaaagacca gatcaaatga cctttttcct tttccaattc ggcttccttg
     1141 atggtgccgt tggggacttt gctcatggcg atttgccggg cctcggcttc agagattttg
     1201 gccttcgatt tcaggtccga ttcgctttcg caggcggcaa gcaggcccgc aagcgcggtg
     1261 atggtcacca atgtggggat tattctggtt attttcatgt gttgattggg tttaccgcgg
     1321 actcacatcc gcgaacctgc atcatgccat aaggaaatga aatggccatg aaatgagcat
     1381 gacagaattg tcatttcacc ctcgcaattt gcgattagat gagcctccct gcctccccaa
     1441 tgtccctccg agtctgctat gtggccgttg gacaaacccg taaggggatt gtattgttgt
     1501 ttgccataag ccaaacgtcc cacatgcttg gacaatagct taatgccgct ggaataaggt
     1561 tttttgatgc aattccagat ccaatgtaac gatcagcctg tgcaggctgt agatgctttc
     1621 ggggcgccat cgccgtcatg gcaggcgaat gacgctccat ctggcggaaa aaaaaccaaa
     1681 aacaagcggt ttctgatttc attctggcgg caaactgttt aaatattttc tgtcgggcaa
     1741 aaaccgggaa atttcagggt gcgttatgcc ggatttgtgt ccggtcattc cgccggataa
     1801 aatttatcag aaagcgagtt tcaacggcgc atgagcagca aactgcacat attgatcgtc
     1861 gaggattccg aagatgacgc aatatttgtc gtgcgagaat tgcaacgcgg cgggtttcaa
     1921 cccgtcgtca agcgagtgga cactgcgcct gatttcgaag ctgcgctcgc ggcgggcggt
     1981 tgggacctca tcatagcgga ctacaacctc ccgcaattca gcgccgtcga tgcgctcgaa
     2041 actcttcaaa aaagtggcca ggaccttccc ttcctcatcg tttccggagc catcggtgaa
     2101 gacgtcgccg tgggcgtcat gaaatgcggc gcgcatgatt acatcctgaa aaataatctc
     2161 acgcgcctcg tccccgccgt gcagcgcgaa ctgcgcgatg cccttgcccg ccgcgaacgc
     2221 cggcaggcgc agcaggaata tcttcgcatg gccgccattg ttgaatcttc cggcgacgcc
     2281 atcatcggca aaacgctcga cggcaccatc accagttgga atatcggcgc ggaacgtctt
     2341 ttcggccatc gtgcggaaga agcgatcggc cgctccattg cggtgctcgt gccgtccgat
     2401 cgcacggagg aggaagaacg gattttggaa cggttgcggc gcggaggaat cgttgatcat
     2461 ttcgaaacga agcgcgtttg caaagacggc cgagtcatcg acgtttccat caccagttcg
     2521 cccatccgcg acaaggcggg aaacatcgtc ggcgcttcca agatcgcgcg cgatatcagc
     2581 gaacgcgtcc gcgcgcaaaa ttacctcgcc gcgctttcga cattgggcca aagcctcgcc
     2641 acagtttctt cgccggagga ttctgcccgc gttattggca acatcgccga tgaattgttc
     2701 ggatgggacg ccttctcgct tgacctttac ttcaccgatc aggaccagat ccagacggtg
     2761 ctcaacgtgg acaccattga cggcgagaag cgcgatgtgc cctgcacgta tattggaaac
     2821 gcccccagcg gcgttgcgcg gcgcatcatt gattccggcg cggaattgat ccttcgcgac
     2881 gaccccaccg cgacgctccc cgatgccatt ccgttgggaa atgtggaacg cccttcggct
     2941 tcgttgatgt tcgtccccat ccgcaaccag acgcgggtca tcggcgtgat gtccgtgcaa
     3001 agttaccggc ccaaggctta tgacgcgcgc gcgttggaca cgctgcaaac gcttgccgat
     3061 tattgcggcg gcgcgctcga acgcatcaag gcgcggcagg agttggaaat ttcccgcgaa
     3121 caattgcgcg cgctggcggc ccatttgcaa tccatccgcg aagaagagcg caagctcatc
     3181 gcccgggaaa tccatgatga actcggtcag gcgctcaccg gtttcaagat ggacctcgcc
     3241 tggatgcgca accggatgca g
//
LOCUS       DASZMM010000231         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3647797, whole genome shotgun sequence.
ACCESSION   DASZMM010000231 DASZMM010000000
VERSION     DASZMM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3647797"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VG754_05450"
     CDS             complement(<1..765)
                     /locus_tag="VG754_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF18689.1"
                     /translation="MKLLKALLCALGLAFVARLPAAEIITSDVCVYGGTAGGVVAAVE
                     AARLGKTAVIAEFGYHLGGMTSGGLSQTDIGNKAAIGGISREFYQRIGQHYGKPEQWT
                     FEPGVAENIFFQMVNEAKVPVYFHQQLASVKKERGRIVEITMENGKVYRAKMFIDASY
                     EGDLMAKAGVSYTVGRESNSQYNETLDGIRPQTPKHQFSVPVDPYKKPGEPNSGLLPL
                     IQSGDLGTPGEGDHCVQAYNFRMCLTQNPGNKRSITA"
     gene            complement(762..1286)
                     /locus_tag="VG754_05455"
     CDS             complement(762..1286)
                     /locus_tag="VG754_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HWF18690.1"
                     /translation="MQSHELLKEVFQKCSAKQVAAELGLSLSMIYKWAEPPDPAAGSG
                     TVNPLDRIDALQRCSADPRIVQWICQRTGGFFIKNPKTHNAHPAFLIPATNEIIQEFA
                     DLLAVIATAVKDSQITPAEAEDIRGRWEELKSVTEEFVQCCEKGNFRHLKENPNAVSL
                     SKSAAAGIKPSAQK"
     gene            complement(1472..1807)
                     /locus_tag="VG754_05460"
     CDS             complement(1472..1807)
                     /locus_tag="VG754_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWF18691.1"
                     /translation="MEPRIVKLDPPNGAQDVGTGVSELRVTFNVPMSGGCSWCTAGED
                     DSDFPKGPDGKRAYWTDDKKTCVLPVILKPGMTYRLRLNAPGFNNFQSEAGVPLVPVA
                     YSFKTAEPK"
     gene            2040..>3320
                     /locus_tag="VG754_05465"
     CDS             2040..>3320
                     /locus_tag="VG754_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HWF18692.1"
                     /translation="MVYFGKYESRMASSLFCSSLDWFRPVAKPRAKFVFWPVLLLPFL
                     LTVPNLSAATNDDKPEFNADIRPILSDSCFVCHGPDKNNRKAKLRLDVREVALEKKAI
                     VPGKPDESEMIRHIYATNRDDLMPPPDSRKQLTSEQRALLKRWIASGAEYQPHWAYIP
                     PVRAKIPQTQNQAWVKNPIDAFVLRDLEKHKIDPALEADKGTLLRRLSLDLIGLPPTP
                     GEVTQFIKDTSPKAYEREVDRLLASPHFGERMAVEWLDLARFADTVGYHGDQNVNDFP
                     YRDYVINAYNSNKPFDQFTIEQLAGDLLPNPTTEQLVASGFNRLNMVTREGGAQPTEY
                     IAKYGADRVRTVSTTWLGSTMGCCECHDHKFDPFSTRDFYSMKAFFADIKQWGVYSDY
                     GSSPNPELKGFDNDHPFPPEIIVDSPYLRNRLDPI"
BASE COUNT          718 a          990 c          858 g          754 t
ORIGIN      
        1 cgcggtaatc gaacgtttgt tccccggatt ttgcgtgaga cacatccgga aattatacgc
       61 ttgcacgcaa tggtcgcctt cgccgggtgt gccaaggtcg cccgattgaa tcaaaggcaa
      121 caagccgctg tttggctcgc ccggcttttt gtacggatca acgggaacgc taaattgatg
      181 cttcggtgtc tgcggacgga tgccatcgag cgtttcgtta tattgcgaat tcgattcgcg
      241 cccaacggtg taagacaccc ccgctttcgc catcaagtcg ccttcatagc tggcatcaat
      301 gaacattttg gcgcgataaa cttttccgtt ctccatcgtg atttccacga tgcgtccgcg
      361 ctcctttttc accgaagcca attgctggtg gaaataaacc ggcaccttcg cctcgttgac
      421 catctggaaa aaaatattct ccgcaacgcc cggctcgaag gtccattgct ccggctttcc
      481 ataatgctgg ccgatgcgct ggtaaaactc cctcgatatg ccgccgattg ccgctttatt
      541 gccgatgtcg gtctggctga gtccgccaga agtcattccg cccaaatgat agccgaactc
      601 ggcgatgaca gcagttttgc cgagtcgcgc cgcttccact gccgcgacaa caccgcccgc
      661 agtgccgcca taaacgcaaa catcgctcgt gatgatttca gcggcgggca ggcgcgccac
      721 aaacgccaag cccaaagcac agagcagcgc cttcaacaat ttcatttctg cgcggacggc
      781 ttgatgccgg cggctgctga ctttgaaagc gaaactgcgt tcgggttctc tttcaggtga
      841 cgaaaatttc ctttctcgca acattgcaca aattcttccg tgaccgactt caattcctcc
      901 cagcgtccgc gaatgtcctc agcttcggcg ggcgtgatct gactgtcctt caccgccgtg
      961 gcgatgaccg cgagaagatc ggcgaattcc tggatgattt cgtttgttgc cggaatgagg
     1021 aaagcaggat gcgcattgtg cgtcttgggg ttcttgatga agaacccgcc ggtgcgctgg
     1081 caaatccatt gaacgatgcg cggatcggcg ctgcaacgtt gcaaggcgtc aatgcggtcg
     1141 agcggattga ccgtgccgct gcccgccgcg gggtcgggcg gttcggccca cttgtagatc
     1201 atggaaagcg aaagccccaa ttccgcggcg acctgtttcg cgctgcattt ctggaaaact
     1261 tccttcaaca attcatgaga ttgcatggca tgaaaatgca gagagcggct cgcattggca
     1321 aggctggagt taaaaagtta ccaaggcgga aacatcgaac gtccaacgcc gaaggctgga
     1381 catcgaatga aggccatagc tccgatttca acgcaaagtc gcaatgacgc aagtgatgct
     1441 tttactttgc gcctttgcgt tcgattcccg attacttcgg ctccgcagtc ttgaacgagt
     1501 acgccaccgg cacgagcggc acgccggctt cgctctggaa attgttgaac cccggcgcat
     1561 tcagcctcaa gcgatacgtc atccccggct tgagaatgac cggcaacacg cacgtcttct
     1621 tgtcgtccgt ccaatatgcg cgcttgccgt caggtccttt cggaaaatca gaatcatctt
     1681 cgcccgccgt gcaccacgag caaccgccac tcatcgggac attgaacgtg acgcgcaatt
     1741 cgctcacgcc cgtgccgaca tcctgcgccc cgttcggcgg gtcgagtttt acaatccgcg
     1801 gctccattgt cttcgcattg acctcttcac ccgcgccgcg cgtggcggga aagtgaatgc
     1861 gcagaagcgg tgagcgtttc gtctttggag cgggtgtttg gcggctcttt gaaaccaatg
     1921 gtggcaagcc gaattgcgcc gccattccaa tggctgggca attgtcccca tgaatgcgcg
     1981 cccctttagg cgcgtctgtc ctttgaaacc tcaaaaacgc tggtcgcgtc tttatattta
     2041 tggtttactt tggaaaatac gagtcgcgca tggcatcgtc tctcttctgt tcatctttgg
     2101 attggtttcg ccccgttgcg aagccccgcg cgaaatttgt tttttggccc gtcttgcttc
     2161 tgccattctt gctgacggtt ccaaatcttt cggcggcgac gaacgatgat aaacccgagt
     2221 tcaacgccga catccgtccg atcctttccg acagttgctt cgtctgccat ggcccggaca
     2281 aaaacaatcg caaggctaaa ttgcgcctcg atgtgcgcga agttgcgctc gagaagaaag
     2341 ccatcgttcc cggcaagccc gacgaatcag aaatgatccg gcacatttac gcgaccaatc
     2401 gcgacgacct gatgccgccg ccggattcac gcaagcaact cacttccgaa caacgggcat
     2461 tgctgaaacg gtggatcgct tccggcgccg aatatcaacc gcattgggcc tacatcccgc
     2521 ccgttcgcgc aaaaattccg caaacgcaaa atcaagcctg ggtgaaaaat ccgattgatg
     2581 ccttcgttct gcgcgacctt gaaaaacaca aaatcgatcc cgcgctggaa gcggacaagg
     2641 gcacgttgct gcgccggctc agcctcgatt taatcggcct tcctcccacg ccgggggaag
     2701 tgacgcaatt tatcaaggac accagcccca aggcttacga acgcgaagtg gaccgtttgc
     2761 tcgcgtcgcc gcacttcggc gaacgcatgg ccgttgagtg gcttgacctc gcgcgtttcg
     2821 ccgacacggt cggttatcac ggcgaccaga atgtgaacga ttttccctac cgcgattacg
     2881 tcatcaacgc ttacaactca aacaagccct tcgaccaatt caccatcgaa caactcgccg
     2941 gcgatctgct gcccaatccc acaaccgaac aactcgtcgc cagcggattc aaccgcttga
     3001 acatggtcac gcgcgaaggc ggcgcgcagc caaccgaata catcgccaaa tacggcgctg
     3061 atcgcgtgcg gacggtttca acgacgtggc tcggctcgac gatgggctgc tgcgaatgtc
     3121 atgaccacaa gttcgatccg ttttcgacgc gcgatttcta ttcgatgaag gcgttttttg
     3181 ccgacatcaa gcaatggggc gtttacagtg attacggttc gtcgcccaat ccggaattga
     3241 aaggtttcga caacgatcat ccgttcccgc cggaaatcat tgtggacagc ccttatttgc
     3301 gtaatcgtct cgacccaatc
//
LOCUS       DASZMM010000232         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2008640, whole genome shotgun sequence.
ACCESSION   DASZMM010000232 DASZMM010000000
VERSION     DASZMM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008640"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VG754_05470"
     CDS             complement(<1..887)
                     /locus_tag="VG754_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWF18693.1"
                     /translation="MTHKAPSLFDWKIAGPAIGDAFRKLHPRAMIKNPVMFVTLVGAA
                     LTTVDIFLPTNERGVIIQIAIWLWFTVLFANFAEAVAEGRGKAQAKALRGTRTQTAAN
                     KITGKGKIETVDANSLRKGDMVVVRASEVIPGDGEVTEGAATVDESAITGESAPVVRE
                     SGGDRSAVTGGTRVLSDEITVRITADPGHGFLDRMIALVEGAKRQKTPNEIALTIMLS
                     ALTLIFMFVIMALKPMGIYAAATFSVPVLIALLVCLIPTTIGGLLSAIGIAGMDRLLQ
                     RNVLAMSGRAVEASGDVDVL"
     gene            complement(1022..2821)
                     /gene="kdpA"
                     /locus_tag="VG754_05475"
     CDS             complement(1022..2821)
                     /gene="kdpA"
                     /locus_tag="VG754_05475"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWF18694.1"
                     /translation="MRNFDWLQLTLYVGVLVLITKPLGLYLVHVLDADGKTWLDWLVR
                     PCERITYRVLGVDPKQEHDWKRYTIAMLIFSLVSTLFTYALLRLQHLLPLNPQKFGAL
                     TPDLAFNTSVSFATNTNWQNYGGESTMSYLSQMVALVIHNFASAAVGIALAAALVRGI
                     ARHTAKTIGNFWVDLVRITYYLLLPVCVVFAVVLVSQGMIQNFKANDTAKLTEPFTVQ
                     VQKTDDKGQPVNGPDGKPIMVDQTVDTQIIPQGPVASQVAIKMLGTNGGGFFNANAAH
                     PFENPTPLSNFLQMLSIFAIPSALTYYLGRMTKNQGHGWAVWGAMFSLFLAGVLVCWW
                     AESNGNLVHQKLGVAVADGNMEGKEVRFGIFNSALFATVTTDASCGAVNSMHDSFTGL
                     GGLVPMLNIQLGEVVFGGVGAGLYGMLVFVVIAVFIAGLMVGRTPEYLGKKIGAYEVK
                     VALLSMLVLALSILGFSAWACVSQWGLAGLNNAGPHGFSEILYAFTSCNGNNGSAFAG
                     LSGNTPWYNTTLGLATLIGRFLMIVPIMALAGALASKKIVPQSAGTFPVHGFTFGALL
                     VGTVVLVGALTYLPALALGPVVEHFLTLQGKLF"
     gene            3327..4085
                     /locus_tag="VG754_05480"
     CDS             3327..4085
                     /locus_tag="VG754_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWF18695.1"
                     /translation="MKAIILAAGKGTRMRELTNELPKPMLKVHGKPILEHIIEGIAAA
                     GVREFFIVTGYKAEVIENHFGDGSKWKSRIAYGRQTVQDGTGKAPELAREFIGASPFF
                     LSYGDILVKSETYQQMLRRYNEDYFSGVLTVTGSQDVTLGGLVFFDEKFRLKRLVEKP
                     SPAQLEQLRKDGSLKPGDIAWYNAGIYIFRPSLFEFTAKLQKSPRGEYELTEAINALA
                     ASHHDIAGMEIAGRWVDVRDPEVLAKLESRSGVS"
     gene            4254..>4711
                     /locus_tag="VG754_05485"
     CDS             4254..>4711
                     /locus_tag="VG754_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18696.1"
                     /translation="MKCLLRFILLVGLVVGWNMHALAAGQTNIQPPGIAYLTPQEEAR
                     TFVCEDGYKMELVLSDPIISDPVITVFDGNGRMFVAEMRSFMRDADGSNERARTSRIS
                     VHWSSKHNGVYDKHAVFLDNLLLPRMILPLADGILVNETDTDDIWYYSDS"
BASE COUNT         1108 a         1290 c         1288 g         1025 t
ORIGIN      
        1 agcacgtcca cgtcgccgga agcctcgacc gcgcgtccgc tcatcgccag gacattgcgt
       61 tgcagcaacc ggtccatgcc cgcaatgcca atcgcgctga gcaatccgcc gatggtcgtc
      121 gggatgagac agaccagcag cgcaatcaat actggcacgg agaatgtcgc cgcggcgtaa
      181 attcccatcg gcttcaacgc catgatgacg aacatgaaga tcaacgtcag cgcggagagc
      241 atgatggtga gcgcgatttc attcggtgtc ttctgacgtt tggcgccctc gaccagcgcg
      301 atcatccggt ccaaaaatcc atggccggga tctgcggtga tgcgcaccgt gatttcatcc
      361 gacaacacgc gtgtgccgcc ggtgacagcg ctgcggtcac caccgctttc acgcacaacc
      421 ggggcggatt cgccagtgat ggccgattcg tccacggtcg ccgcgccttc ggtgacttcg
      481 ccgtcgcccg gaatcacttc actcgcgcga acgaccacca tgtcgccttt gcgcagactg
      541 ttggcatcca cggtctcgat cttgcccttg ccggttattt tgttcgcggc ggtctgggtg
      601 cgggtgccgc gcaaggcctt ggcttgcgct ttgccgcgcc cttcagcgac agcttcggca
      661 aaatttgcga acaacacggt gaaccagagc cagatagcga tttgaatgat gacgccccgt
      721 tcgttggttg gcaaaaaaat gtccacagtc gtcaacgccg cgcctacaag cgtcacgaac
      781 atcaccggat ttttgatcat ggcgcgggga tggagcttgc gaaacgcgtc accaatcgcc
      841 gggccggcaa ttttccaatc gaaaagtgat ggagctttat gggtcataaa atgcaaaaca
      901 tccaacgctg aacatcgaac gctgaacgcc cagtgacccg atggatgcgt tttgattgtt
      961 cgatgttcga cgttcgatgt tggatgttca gagttgttgt tttcatgctg agttcgacct
     1021 ttcagaacag cttgccttgc aatgtgagga aatgttcgac gacgggtcca agcgcgagcg
     1081 ccggcagata ggtcaacgcg ccgaccaata ccacagtgcc gaccagcagc gcgccgaaag
     1141 tgaaaccatg caccgggaaa gtgccggcgc tttgcggaac gatttttttc gacgccaatg
     1201 cgccggccaa cgccatgatg ggcacaatca tcaaaaaacg cccaatcaaa gtcgccagtc
     1261 caagcgtggt gttgtaccat ggcgtgttgc cggacaaccc ggcgaacgcg ctgccgttat
     1321 ttccgttgca ggaggtgaac gcatataaaa tttcgctgaa accatgcggg cctgcgttgt
     1381 tcaatcccgc cagtccccat tggctcacgc aagcccatgc gctgaatccc agaatggaca
     1441 acgcaagcac cagcatggag agcagcgcga ctttgacttc ataagctccg attttttttc
     1501 caagatattc cggcgttcgc ccgaccatca atcccgcgat gaacactgcg atcaccacga
     1561 acaccagcat tccataaagg cccgcgccga cgccgccgaa caccacctcg cccagttgaa
     1621 tgttcaacat cggcaccaac ccgcccaaac cggtgaacga atcgtgcatc gaattgaccg
     1681 caccgcagga tgcgtcagtc gtgacagtgg cgaacaaagc cgaattgaaa ataccgaacc
     1741 gcacttcctt gccttccatg ttgccgtccg caactgcgac accgagtttt tgatgaacga
     1801 gatttccgtt tgactcagcc caccagcaga ccagcacgcc cgccagaaac agcgaaaaca
     1861 tcgcgcccca cacggcccag ccgtggcctt ggttttttgt catgcggccc aggtaatagg
     1921 tcagcgcgct cggaatggcg aagatggaaa gcatctggag aaaattggaa agtggcgtcg
     1981 ggttctcgaa cggatgcgcc gcattcgcat tgaaaaatcc gccgccgttc gtgccgagca
     2041 ttttgattgc gacctgcgaa gcgaccgggc cttgcgggat gatttgcgtg tccacggttt
     2101 gatccaccat tatcggcttc ccgtcagggc cgttgacggg ctggcctttg tcatcggttt
     2161 tttggacttg gaccgtgaaa ggttcggtga gcttggcggt gtcgttggcc ttgaaatttt
     2221 ggatcatccc ttgcgatacc aggacgactg caaataccac gcatacaggc agcagcagat
     2281 aataagtgat gcgcacgagg tccacccaaa agttgccgat tgtttttgcc gtgtgccgcg
     2341 cgatgccgcg caccagcgcc gccgccaagg caatgcccac cgcggctgaa gcaaagttat
     2401 ggatcaccag cgcgaccatt tgggacagat acgacatcgt ggattcaccg ccgtaattct
     2461 gccaattggt gttcgtggcg aaactcacgg aggtgttgaa cgccaggtcc ggcgtgagcg
     2521 cgccgaattt ttgcggattc aacggcagca gatgttgaag ccgcaagagg gcgtaagtga
     2581 acagcgtgct caccaggctg aaaatcagca tcgcgatcgt gtagcgtttc cagtcgtgct
     2641 cttgtttcgg atccacgcca agcacgcggt aggtgatgcg ttcgcaggga cgcacaagcc
     2701 agtccagcca ggttttgccg tcggcatcca acacatggac gagatacaga ccgagcggct
     2761 tggtgatcag gaccagaacg cccacgtaga gcgtaagttg cagccagtca aaattgcgca
     2821 taattagatt ttgaaaaggt tttcgttgga gccggttttg cgccggcgat ttttgcgttg
     2881 tgacgcgaac tgcatctcag aatttttccg ggcgtatcat cgcgaccaac aggtagatgc
     2941 acagcgccac ggcaatggtt cccacaatga gattttccat gttgcctccg attcagagct
     3001 tcgcgcacag gacggcgtaa taaccagccg ccgcgaagaa cgccaccgtc accgcaatgt
     3061 aaatgacatc attcatgtgg gtaacatggg gctgcttgcc ccgttggcat aatcaaaagg
     3121 aatgagcaaa ccgttaaaaa aatgctaaaa atccctttta aatcgttgcg attcctttcg
     3181 aaaacttcag ctattgggct ggaaatcaca taaggagcat ttctgccgta ggacagttta
     3241 tgagagtagt ttgcggccgg gcagatgcaa ctcagcagaa ctttaaactt taaattttgg
     3301 actttgaatt tcattatcag aaaactgtga aagcaatcat tctggccgcc ggcaaaggga
     3361 cgcggatgcg tgaactcacg aacgagcttc cgaaaccgat gctcaaagtt catggcaagc
     3421 ccatcctcga acacatcatc gaaggcatcg ccgctgcggg cgttcgtgaa ttcttcatcg
     3481 tcaccggtta caaggcggaa gtgatcgaaa atcatttcgg cgatggttcg aaatggaaat
     3541 cgcgcatcgc ttatggccgt cagaccgtcc aggacggcac cggcaaagcg ccggaactgg
     3601 cgcgtgaatt tatcggcgct tcgccgtttt ttctcagcta cggggacatt ctggtgaaat
     3661 ccgaaacgta tcagcaaatg ctgcggcgct ataatgaaga ttatttttcc ggcgtcctca
     3721 ccgtgaccgg cagccaggac gtgacattgg gcggccttgt ttttttcgat gaaaaattcc
     3781 ggttgaaacg cttggtggaa aaaccaagcc cggcgcagtt ggagcaattg cgcaaagacg
     3841 gttcgctcaa gcccggcgac attgcctggt ataacgcggg gatttatatc ttccgccctt
     3901 cgcttttcga attcaccgcc aagctccaaa aatctccgcg cggcgaatac gaattgaccg
     3961 aagccatcaa cgcgcttgcc gcatcgcatc atgacatcgc cgggatggaa atagccgggc
     4021 gttgggtgga tgtgcgcgat ccggaagttc tcgccaaact tgaaagcaga tcgggcgtca
     4081 gttgaggtcg aatttgagag tcgggcattt gcggaggctt tcaatgcgaa gcagaaaggg
     4141 gtttcgatgc actttgagtg cttttgcaag ttaactgtcc aattctggca tttaaaaggc
     4201 tggactgggc tgccattccc agcaaaaata agccaaatgt cgataacggg tgtatgaagt
     4261 gtcttcttcg atttatattg ctggttggtt tggtggttgg ctggaatatg cacgctttgg
     4321 cggcaggcca aaccaatatc cagcctcccg gcatcgcgta cctcaccccg caagaagaag
     4381 ccaggacttt cgtttgtgag gatggttaca aaatggaact ggtgctgagc gatcccatca
     4441 tcagcgaccc cgtcataact gttttcgatg gcaacgggag aatgttcgtg gcggagatgc
     4501 gcagtttcat gcgggacgcg gatgggagca acgagcgcgc gcgaaccagc cggatttccg
     4561 tgcattggtc gagcaagcac aacggagttt acgataagca cgctgtcttc ctggacaatc
     4621 tgctgctccc gcgcatgatc ctgccgctcg cagacggcat tcttgtcaat gaaaccgaca
     4681 cggacgacat ctggtattat agcgactcca a
//
LOCUS       DASZMM010000233         4313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1740292, whole genome shotgun sequence.
ACCESSION   DASZMM010000233 DASZMM010000000
VERSION     DASZMM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740292"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VG754_05490"
     CDS             <1..982
                     /locus_tag="VG754_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HWF18697.1"
                     /translation="TSKGIDWSGTLSAQHVTAGNNVTGGASGFPYGQTFQSTVVQGLF
                     GSGTNTQTIPVGVINQTTYTPNNLVQNPGIIADTAHGMDPHTMFLNADGVSAVLSFLN
                     TEADAKVLSTPRAVTLDNQEAVLSVTTAEPIFKTTAGTQGSPGGAEVTYTNLGTILKV
                     TPRISANNYINLRVVPEVSDLGGTITKTVSGLTSQADFFDIRKIDTRVLIPSGNTLVM
                     GGLVSDQSSKGNTKVPFLGDIPLLGYAFRSESKTQNKKNLIIFVTPTIVQNEDFQPTE
                     TAFLKTKPPESKGSDFSAWDSGAPQDWSKLMHPKKAANTDDSYSDFPTTK"
     gene            complement(1156..1458)
                     /locus_tag="VG754_05495"
     CDS             complement(1156..1458)
                     /locus_tag="VG754_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWF18698.1"
                     /translation="MRPLDIQPIGEELAIKWEDGSESFVRLEKLRQHCPCAGCKGEVD
                     IMGNVYKGPEQKLDLRAFQLLRLGTVGTYAVQPFWADGHSTGIFSFDYLRKVAEAE"
     gene            complement(1702..2418)
                     /locus_tag="VG754_05500"
     CDS             complement(1702..2418)
                     /locus_tag="VG754_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWF18699.1"
                     /translation="MIPKKRTRRAPDLPFVFLNVATTADGKLAPANRHFVPFSSKRDQ
                     EHLLELRATADAVMSGARTVDLSAVKLGTGGARHRRQRLKRGLAEYNLRVVVSGAGTL
                     DPRAEIFKHRFSPIIILTTGRASRKKLRQLEKLADVVKICGERELDFEFALRWLRREW
                     GVKRLLCEGGGEINAGLFEAGLVDEIHLTLCPVIFGGRIAPTLSDGRGFERLADATLS
                     RVKSKKRIGDEMFLVYEVLR"
     gene            3519..4292
                     /locus_tag="VG754_05505"
     CDS             3519..4292
                     /locus_tag="VG754_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18700.1"
                     /translation="MKKILVRIAIGLAVLIVVALLVVFFSLNSIVKKGVETVGPMITK
                     VDVKLSAAEISPFSGSGRLKNLVVGNPEPFKAPSSMQFGDMKVGVQLGSLASDVIVVN
                     EVNVQDAEITLEGGLKGNNLSKLLDNINSSSAEAGNQPKPKNAPPTAGASKSSKKFIV
                     KDILIQGTKVHAALDIPGLGTQTFTLPLPPLHLQNIGEAEHGVTAEQLTQAIMKPLFE
                     NIMTAVTQQVGNLGGSLKGLGTNGINSVGKSIGNLFKKQ"
BASE COUNT         1019 a         1252 c         1060 g          982 t
ORIGIN      
        1 cacatccaag ggtattgact ggtccggaac gctgtcggcc cagcacgtta cagccggaaa
       61 taacgtcacg ggcggcgcct cgggatttcc gtatgggcaa acatttcaat cgacggttgt
      121 acagggcctc tttggcagcg gcacaaacac tcaaaccatc ccggtgggcg tcatcaatca
      181 gacgacttac acaccgaata atcttgtcca aaaccctgga atcatcgcgg acacagcgca
      241 tggcatggat cctcatacga tgtttcttaa tgccgatggc gtgagcgcgg ttctctcctt
      301 cttgaacacg gaagcggatg ccaaagtgct ctccacgccg cgcgcagtca ccttggacaa
      361 tcaggaagcc gtcctttccg tcaccaccgc cgaacctatc ttcaagacca cagccgggac
      421 acaaggctcg cccggcggtg cggaagtcac ctataccaac ttgggcacca tcctgaaggt
      481 caccccgcgc atctcggcca acaactacat caatttgcgc gtcgttcccg aagtctcgga
      541 tctcggcggc accatcacaa aaactgtcag cgggctgacc agccaggcgg acttcttcga
      601 catccgcaag attgacactc gcgtcctcat ccccagcggc aacacgctcg tgatgggcgg
      661 tttggtgagc gatcaatcca gcaagggcaa caccaaggtc ccattcctgg gtgacatccc
      721 gctgttgggc tacgctttcc gctcggaatc gaaaacccag aacaaaaaga acctgatcat
      781 cttcgtgacg ccgaccattg tgcagaacga ggacttccag cccacggaaa ccgcgttcct
      841 caagaccaaa ccacccgaat cgaaaggctc ggatttcagc gcctgggaca gcggcgctcc
      901 tcaagactgg agcaagctca tgcatcccaa gaaagccgcg aacaccgacg attcctactc
      961 ggattttccg acgaccaagt aattctgttc cagttatccc aaccaccgcc ttataatcaa
     1021 ggcggtggtt tttctttttg aaagaaacat cggacgctga acatcgaaca tgcaacttcg
     1081 aatttaagat tggaacttcg gcgcatttca ttggtcgttc ggcgttggat gttcgacgtt
     1141 tgcccatttc actcttcact ccgcctccgc caccttgcgc aggtaatcaa aagaaaaaat
     1201 tcccgttgag tggccgtcag cccaaaatgg ctggactgcg taagtcccca cggtgccaag
     1261 gcgcaacaac tggaacgcgc gaaggtccag tttctgttcc ggtcccttgt aaacattccc
     1321 catgatgtcc acttctcctt tgcatccagc gcagggacaa tgctggcgca gcttctccaa
     1381 tcgcacgaaa ctctcgcttc catcttccca tttgatggcc agttcctcac cgatcggttg
     1441 tatatccaaa ggtcgcatga ctgaatcact gctaactcaa attatgcata ttactataag
     1501 taaacaggtt gctcagaagc ttcatgatgg aatcatctaa tacgttcaac gtactattgc
     1561 ccggcaaaat aaccattgac ttaaacttca tcatttacac aatcgttttc ggcccgggtg
     1621 ggcggcacgt ttcttcagcg ggattcccca tccccacctc cttggcgtcc gccgcccggg
     1681 tcatctggga ctttgcgcgc ctcatcgcaa cacctcatat accaggaaca tttcatcgcc
     1741 gatccgcttc tttgatttta cccttgatag tgtcgcatcc gccagacgct caaatccccg
     1801 tccgtccgac aatgtcggcg cgatgcgacc gccaaaaatc accgggcaaa gcgtcaggtg
     1861 gatctcatcc accaaccccg cctcgaacaa tcccgcgttg atctcccctc caccttcgca
     1921 caacaaccgc ttcacacccc actcgcgccg aagccagcgc aacgcaaatt caaaatccag
     1981 ttcgcgttcg ccgcagattt tcacgacgtc agccaacttt tccagttgcc gcaatttctt
     2041 tcgcgacgcg cgcccggtcg tcaggatgat gatcggcgag aaacgatgtt tgaaaatttc
     2101 cgcgcgcggg tccaacgtcc cggccccgct caccacgacc cgcagattat attccgcgag
     2161 gccgcgcttg agccgttgcc gacgatgcct ggcaccgccc gttccgagct tgacggcgct
     2221 caggtccacc gtgcgcgcac cggacatcac cgcatcggct gtggcgcgca gctccagcaa
     2281 atgctcctgg tcgcgcttgc tgctgaacgg gacaaaatgc cgattcgcag gcgcgagctt
     2341 gccatccgcc gtcgttgcca cattcaaaaa gacaaacggc aggtctggcg cgcgtctggt
     2401 tcgtttcttc ggtatcattg aatcattacc aatcaatcgt ttccgaaacc aaatgacaag
     2461 ttttcactcg ccgagcgaca ttaagcgaca tggtcaggga aaagaatttt tttcatctta
     2521 gcgttaatta gcgttaatta gccttaattt tccgatgctg gatgctgagt gtcggagttc
     2581 ttcattccgc actccgcaat ctacattccg cacttggcgg ggggtgtcgt ttttcggcgg
     2641 gtttaggtga agctatgcgg gtttaagcgg gtttacaacc acttacaact tgaacgctct
     2701 gtgcaccgta gctgaaaacg tgagttttca gatcgtgggc cgagcctata gcccgtgcgg
     2761 aagcaccccg tctggaaaga cctcgcgagc cgttccagat ttgattccgc cgaaccaagc
     2821 aaaaatattt tattttttta acccggttta tccgagaata cgcgagaata gctgggaata
     2881 cccgtggtaa gctgagaatt ctgatgtaag aatattccag gcaagtcgag ttttcagcca
     2941 tttcaaaccg gatgcttgat acccgattcc ggatttcttc attccgcaat cggcaatccg
     3001 cactctaaat ttcaaagagc ttcctccttc caacgccaac gagagtatca caggcatttt
     3061 tgaaggcaag aaatttcgcc aggattttat ggcatccggg tttaccgggg gtcgcgggaa
     3121 tattccgaac agcgggaaat ggtgaaagcc ctctcctagg ggagagggtt tgggtgaggg
     3181 cgagcgtcat ccaaaccgta ccctgtaaac cattgccgcc aatgataaat gggcggatgc
     3241 cggttctcct tctccaaggg gaccacacaa cgcgaagcgt tcgtctcttt tggggaaact
     3301 gacttgtggc gggcttggga agccattagg ccgggatgag ggcgggcctt ctctcgcttc
     3361 tcacaatgga ttcaagaaca gtgtcaagat accccacgta tcccaaatct cgacattcca
     3421 cttgaccagc ctgtttgcag tggcacattt gattgagcca ctgggtttta tactgcgcaa
     3481 ttgctacagg gtggcccggg tattcaaagt ttaattgtat gaagaaaatt cttgttcgca
     3541 tcgccattgg cctcgctgtg cttatcgtgg tcgcgttgct cgttgttttc ttttcgctca
     3601 actccatcgt gaaaaaggga gttgaaaccg tcgggccgat gatcaccaag gtggatgtca
     3661 agttgagtgc ggcggagatt tctcctttct ccggaagtgg acggctcaag aatctggtgg
     3721 tcggcaatcc cgagcctttt aaagcgcctt cgtcgatgca gttcggcgac atgaaggtgg
     3781 gcgtgcaact cggttcgctt gcctccgatg tcatcgtcgt caatgaagtg aatgttcagg
     3841 acgcggaaat caccttggaa ggcgggttga agggaaacaa tctcagcaaa cttctcgaca
     3901 acatcaatag ttccagcgcc gaggcgggca accagcccaa gccgaaaaat gcgccgccca
     3961 ccgccggggc cagcaaaagc tcaaaaaaat tcatcgtcaa agatattttg atccagggca
     4021 cgaaggtgca tgccgctttg gatataccgg gcttgggcac gcaaacattc accctgccgc
     4081 tgcccccgtt gcatttgcag aacattggcg aggccgagca cggcgtcacc gccgagcaac
     4141 tgacgcaggc catcatgaaa ccgctgtttg aaaacatcat gacagcggtc acgcagcagg
     4201 tcggcaatct tggcggcagt ttgaaaggcc ttggcacgaa cggcatcaac agcgtgggaa
     4261 agagcatcgg caatctgttc aagaaacagt gatcccgaag ctttcccgta gct
//
LOCUS       DASZMM010000234         5448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_375492, whole genome shotgun sequence.
ACCESSION   DASZMM010000234 DASZMM010000000
VERSION     DASZMM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375492"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VG754_05510"
     CDS             complement(<1..881)
                     /locus_tag="VG754_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF18701.1"
                     /translation="MATPRYLSAFNSRNYRLYFTGQAISLMGTWMTQTASLWLIYHLT
                     HRAFLLGLVGFVSQVPMFVLAPFAGVWIDRVNRHRLLVVTQTLSMLQSFALAAFVFSN
                     KMDAAILLSLSLVQGIVNAFDMPTRQALVIEFIEKKEHLGNAIALNSSMFNLARLVGP
                     AVAGYVIEWSGAGGCYLADGISYLAVIISLLMMRLREQPHRAEHQHPWPALKEGFRFA
                     FGFAPVRALIIVVGLVSFMGFGYTVLTPVFARDVFHGDARTLGHLMAASGVGALLGAM
                     YLGTRTTVRGLGNVITLG"
     gene            complement(935..1180)
                     /locus_tag="VG754_05515"
     CDS             complement(935..1180)
                     /locus_tag="VG754_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18702.1"
                     /translation="MEISKRDYFAAQALTGLLARAGGPSSGLSVHANVAAEAFLYADE
                     MLKLSKTLLDPVEIDPDAMAREAEEQPLRDKPNTKSS"
     gene            complement(1368..1535)
                     /locus_tag="VG754_05520"
     CDS             complement(1368..1535)
                     /locus_tag="VG754_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18703.1"
                     /translation="MKPDLLILFLTVAVGLFAAAFRYFARWRKARSVSPVKLRQKNSA
                     VVRRNRDWRWF"
     gene            complement(1839..3080)
                     /locus_tag="VG754_05525"
     CDS             complement(1839..3080)
                     /locus_tag="VG754_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF18704.1"
                     /translation="MILQAMNNGPATLAAKLKAFPRPVWVLFFGTFLNKFGTFVIPFL
                     ALYLKGRGFSIAQAGMAISAYGIGNFLASFVGGHLADSFGRRNTIVFSMFSVAVSMML
                     LSQAEQFPVILAFTALTGLTGEMYRPAGGALLADLVPAGQRLTAYSAYRLAFNAGWAF
                     GPATAGFLAGHSFFWLFVGDAASSVLFGIVAWVALPHGLRAAKSDQGWTPALKVISRD
                     KSFLRLLAAGFVVSMVFLQMSSTFSLHVTGLGFSPATYGALISLNGVLVVFCELPLTT
                     ITQRFPPRRMMALGAALTGVGFAMNAFAHTLPLLVLSVFVFTLGEMINLPVAAAYVAD
                     LAPTEFRGRYSGAAGLTGALALIIGPNIGMTVFGRSPFALWLGCGALALVGALIMIWG
                     MDSSSKKVEKAAREEFKEERL"
     gene            complement(3171..4067)
                     /locus_tag="VG754_05530"
     CDS             complement(3171..4067)
                     /locus_tag="VG754_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18705.1"
                     /translation="MAHGFFEKIFAARKLVFVLFAVSLAAVRVPVGTAAPVNAANRSD
                     ATGPTLHLDYAHGETNGSPAAEFMYFVPLISPEPVTLSKSAVNTQRARILSVTRRDKG
                     ESFTVTCDMEFSGQGRQQNIFDHTKMIQRRESDLKAGKSLVRLLSSISVEGPGQLQVE
                     VEGKMSGSTSTVNEVRLHFNAHGHESPVVIGLHDVKYIDGKFQPCNGLVARVNTLTFH
                     RTDGPPRMEVSVASVKRADAGDSMWQNFVGDLKGMAVNALIKPLVVERTGNETMLDFG
                     LALTSRAGTFTFPKAKNLKEEP"
     gene            4164..4898
                     /locus_tag="VG754_05535"
     CDS             4164..4898
                     /locus_tag="VG754_05535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18706.1"
                     /translation="MKTTPAPARLCLFLLLFLLGPSFCPAQDTNSASAYTYKAVHDPD
                     GIGKFYQGREIAQVMGHQGADWLERPTRELEEQPALLMGALHLKQGDAVADIGAGTGF
                     YSRRLARAIGTNGVVYAVEIQQEMLDILTNKMAEIGIHNVKPVLGTVTNPRLASNSVD
                     LILMVDVYHEFDHPREMTGAMVDALKPGGRLVFVEFRGEDPKVPIKPLHRMTEAQVRK
                     EMSSFSLKWVETNEALPIQHIIVFRK"
     gene            complement(5064..>5448)
                     /locus_tag="VG754_05540"
     CDS             complement(5064..>5448)
                     /locus_tag="VG754_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWF18707.1"
                     /translation="TPEEREKRKTGAQNKLLEIETSKNSIRTFEDNAREQLDSQRKRM
                     RDSILEDIRTAINAKAKNAGYTIVIDIAAESFNQTPIVMYTNGENDMTETILSQLNVG
                     APPPATSTDVPKTPDKSTNSTSGNK"
BASE COUNT         1286 a         1499 c         1534 g         1129 t
ORIGIN      
        1 cccagcgtga tgacatttcc caatccgcgc acggtcgttc gcgtgccgag atacatcgcg
       61 ccaagcagcg cgcccacgcc cgaggcggcc atcagatgac caagcgttcg cgcatcgcca
      121 tggaaaacat cgcgcgcgaa aaccggcgtg agcaccgtgt aaccgaatcc catgaaactc
      181 accaagccca cgacaatgat gagcgcgcgg accggcgcaa agccgaacgc aaaacgaaat
      241 ccttccttga gggcgggcca tggatgctgg tgttccgccc ggtgcggctg ttctctcaac
      301 cgcatcatca aaagcgagat gatgaccgcc agataactga ttccatccgc aagataacac
      361 cctccggcgc ccgaccattc aatgacataa ccggccacag ccggcccaac cagccgcgcg
      421 agattgaaca tcgaagaatt gagcgcgatg gcattgccga ggtgttcctt cttttcgatg
      481 aattcgatga ccaacgcctg gcgcgtcggc atgtcaaagg catttacgat gccttgaacc
      541 agactcaacg aaagcagtat cgcggcatcc attttattac tgaaaacgaa cgcagccagc
      601 gcgaaggatt ggagcatgga aagcgtttgg gtcacgacaa gcagccggtg acggttgacg
      661 cgatcaatcc aaacgccagc aaacggcgcg agcacgaaca tggggacctg gctcacgaaa
      721 ccgacaagtc ccagcagaaa ggccctgtgt gtgagatgat aaatgagcca cagggacgcg
      781 gtttgcgtca tccaagttcc catcaacgaa atcgcctgcc cggtgaagta gaggcggtaa
      841 ttgcgggagt tgaacgcgct gagatagcgg ggtgtcgcca tggtttgttg gcctcagccg
      901 gagcaggctt gcggccgtaa agacgtggcg cggttcaaga ggacttggtg tttggtttgt
      961 cgcgaagggg ttgttcttcg gcttcgcggg ccatcgcatc gggatcaatc tcaaccggat
     1021 ccagaagggt cttggataac ttcaacattt catcggcata aagaaatgcc tcggcggcga
     1081 cgttggcgtg gacgctaagg ccggaactgg gtccgccagc ccgcgcaagc agccccgtca
     1141 aggcttgcgc ggcgaaataa tcacgtttgc tgatttccat aatttgattg atcgtccggt
     1201 ttcgctatac gccgaaacac gaactaaatc aaggatatgg aacgagcggc gaatggagta
     1261 gtgcagaatt ggagtgatgg agtggaaatt ccaatactcc aattactccg ccactccaac
     1321 ccgatgacag agtcggtggg tgctgtaagg gataaaagac ccgccgatca gaaccagcgc
     1381 caatctcggt tgcggcgaac gaccgcagaa tttttctggc gcagtttgac cggggaaaca
     1441 ctgcgggctt tgcgccagcg ggcgaagtaa cgaaaggcag cagcaaaaag accgactgcc
     1501 acggtaagaa aaagaatcag cagatcaggt ttcataggcg tcgagttaca cagactgcat
     1561 cgcaaagcag cttagttcgt gccagagatt tcactcaggc gaccgcattt cgccaaatct
     1621 cgtttgtttt gaactacttg caacgcaatt gtgtgctgat gccgcacaaa tcattagagc
     1681 gtatttcgta agggaatccg acacatttag gaattatttg agttagactt tcggatttaa
     1741 agcgtcagca actcaatatc tattggcaaa aacgtgattc gtaaaaaaat ccgacgaaat
     1801 ctttcggcga gtgtcatcta tatgcagaat cgctgttctc acagtcgctc ctctttgaat
     1861 tcttcgcggg cagccttttc aacttttttg gatgatgagt ccatgcccca aatcatgatc
     1921 aatgcgccaa ccaaggccag cgcgccacaa cccagccata acgcaaacgg gcttcgccca
     1981 aaaaccgtca tcccgatatt cggcccgatg atcagcgcga gcgcgccggt caatcccgcc
     2041 gcgccggagt agcgaccgcg aaattctgtt ggcgcaaggt ccgccacata agcagcggcg
     2101 accgggagat tgatcatttc tcccaaggtg aaaacaaaca ccgacagcac aagcaacgga
     2161 agtgtgtggg cgaatgcgtt catggcaaat cccacccctg tcaatgcggc gccaagcgcc
     2221 atcatccggc gcggtggaaa tcgttgcgtg atggtggtga gtggcaactc gcaaaagaca
     2281 accagcacgc cattaaggga aatcaatgcg ccgtaagtcg cgggagaaaa tcccaaaccg
     2341 gtcacgtgca ggctgaaggt tgaactcatt tgcaggaaaa ccatcgagac cacgaatccg
     2401 gctgccagca accgcagaaa tgatttgtcg cgcgaaatga ctttcaacgc gggcgtccag
     2461 ccctggtcgg atttcgccgc gcgcaatccg tgcggcaaag cgacccatgc gacgatgccg
     2521 aacaacacgg acgaggccgc gtcgccgaca aacagccaga aaaaggaatg cccagccagg
     2581 aagcccgccg ttgcggggcc aaatgcccat cctgcgttga acgcgaggcg gtaggcggaa
     2641 tacgccgtca ggcgttgacc agcgggaacg aggtcggcca ggagcgcgcc cccggccggg
     2701 cgatacattt cgccggtcaa cccggtcagc gcggtgaacg ccagtatcac cgggaattgt
     2761 tcagcttgcg aaagcagcat catggaaacc gccactgaaa acattgaaaa aacgatggtg
     2821 ttgcggcgtc caaaagagtc tgcgagatgg ccgcccacga acgacgcgag aaaatttccg
     2881 atgccatacg cgctgatggc catgcccgct tgcgcgatgg agaagccgcg gcctttcagg
     2941 tacagcgcga gaaatggaat gacgaatgtc ccgaatttat tgaggaacgt gccgaagaaa
     3001 aggacccaga ccgggcgcgg aaaggccttc aatttcgccg ccaaagttgc cgggccattg
     3061 ttcattgctt ggagaatcaa aaaccccgcc gctgatttgc ggcagggtcg gatatttccg
     3121 gcgcaacgat gcgattaaat ggaatcgcaa gtcaatgccg attcggcagc tcacggttcc
     3181 tctttcaaat ttttcgcctt tggaaaagtg aatgtcccgg cgcgcgatgt cagcgcaagg
     3241 ccaaaatcga gcattgtttc atttcccgtg cgctcgacga cgagaggctt gatgagcgcg
     3301 ttcaccgcca tccctttcaa atctcccaca aaattctgcc acatactgtc tcccgcatcg
     3361 gcgcgcttca cggacgcaac cgatacttcc atcctgggcg gaccgtctgt ccggtgaaag
     3421 gtgagcgtgt tgacgcgcgc taccagcccg ttgcacggct ggaatttgcc gtcgatatat
     3481 tttacgtcgt gaaggccgat gactacgggg ctctcatgcc catgggcatt gaaatgcagg
     3541 cgcacttcat tgaccgtcga ggttgagccg gacatcttcc cttccacttc aacttgaagc
     3601 tgcccgggtc cttcgacact gatggaggac agcaatcgca cgagagattt gccggccttg
     3661 aggtcggatt cgcggcgctg gatcatcttt gtgtgatcga aaatattctg ttgcctgcct
     3721 tgaccggaaa attccatgtc gcacgtcaca gtgaaggatt cgcccttgtc acggcgtgtc
     3781 acggaaagga tccgcgcgcg ttgcgtgttc accgcgcttt tgctgagggt cacgggttcc
     3841 ggtgaaatga gcggaacaaa atacatgaac tccgcggcag gcgaaccgtt cgtttcgccg
     3901 tgcgcgtaat cgaggtgcaa cgtcggaccg gtggcgtccg agcggtttgc cgcgttgact
     3961 ggagcggccg ttcccaccgg cacgcggacg gcggccaacg acacagcaaa caacacgaac
     4021 accagtttcc gcgccgcaaa aatcttttca aaaaaaccgt gcgccatgga atcactttgg
     4081 cgttaatcgc gccgaattca acttcaatcc gaacgggaac gcgaattatt gaagtccgcc
     4141 aactttgcgc ttacactcgc gtcatgaaaa ccacgcccgc tcccgcccgg ctgtgtctgt
     4201 tccttctgtt gttcctactc ggcccatcgt tttgtccggc acaagacacg aattccgcgt
     4261 cggcttatac atacaaggcc gttcatgatc cggacggcat cggcaagttt tatcaaggcc
     4321 gcgaaatcgc acaggtgatg gggcatcagg gcgcggactg gctcgaacgc ccgacacgcg
     4381 aattggaaga acagccggca ttgctgatgg gggctttgca cttgaaacaa ggcgacgccg
     4441 tggcggacat cggcgctggt accggttttt attcacgtcg cctcgcgcgc gcaatcggca
     4501 ccaacggcgt tgtctatgcc gtcgaaatcc agcaggaaat gctcgacatc ctcacgaaca
     4561 aaatggccga aatcgggatc cacaatgtga agccggtcct tggcaccgtg accaatcccc
     4621 ggcttgcttc aaattcggtg gatttgatcc tcatggtgga tgtgtatcat gagttcgatc
     4681 atccgcgcga aatgaccggg gcgatggtgg acgctttgaa acccggtggc cgcctggtgt
     4741 ttgtggaatt tcgcggggaa gaccccaaag tgcccatcaa gccgctgcac aggatgaccg
     4801 aggcgcaagt gcgcaaggaa atgtcttcgt tctcgttgaa atgggtggaa acgaatgaag
     4861 cgctgccaat ccagcacatc atcgtgtttc ggaaataggc ggagcgcgac tgtcctcagc
     4921 cgcagcaatg ggtcattcat cgcgttcctt tcggaaaatg agtccgcccc ttcgatttta
     4981 aaactgttga gttttcggat actgctgcgg ctgaggacag ccgcggtccg ctttccagcc
     5041 ttaagcttaa ttcagcttaa tgattattta ttgccgctgg tgctgttcgt ggatttgtcg
     5101 ggggtcttgg gaacatccgt gctggtggcc ggaggcggcg cgccgacatt gagctgggaa
     5161 agaatcgttt cggtcatgtc gttttcacca ttggtgtaca tgacaatcgg cgtctggttg
     5221 aaactttccg cggcgatgtc aatgacgatg gtgtagcccg cgtttttggc cttggcgttg
     5281 atggcggtgc ggatgtcctc aagaatgctg tcgcgcatcc gtttgcgctg cgaatccagt
     5341 tgctcgcggg cgttgtcctc aaaggtgcgg atggaattct tgctcgtttc aatctccagc
     5401 aatttatttt gcgcgccggt cttgcgcttc tcgcgttcct cgggggtg
//
LOCUS       DASZMM010000235         9708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2567949, whole genome shotgun sequence.
ACCESSION   DASZMM010000235 DASZMM010000000
VERSION     DASZMM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567949"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3018)
                     /locus_tag="VG754_05545"
     CDS             complement(<1..3018)
                     /locus_tag="VG754_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF18708.1"
                     /translation="MKKASFLVFFLLSFATAAFAILPCYEPFANSVGLFPTGTAYAAG
                     SPGWHQTNAQGDFWLEINSTGGGPSIYLTNFSLFYPGLPASTGNAIVLTNAAGAGMRI
                     NTASPQIFETQNGVKLYYSMLVSVPNIASLPTTGDYCWGFNNGSGDQGNQPSDFHARI
                     YFKKNGAGYQLGIARTTSVAYDSTVHSTNETVFIVASYEIVTTNSSGSSSTTNDICRL
                     WINPNTNTFGAAAPPGENTNTIVFTDADETTSPNTFILANRSTTQPNILIVDDFRLGT
                     NWAFVTGAVAVKTPPPATKTVAFGATLSLTNRATGSSSVIYQWQFNGTNIAGATASIL
                     TIANMADPNAGTYSSIVSTGIGSSITNATVVTVTGDPQFVLQPVNLNAPTGGGGSFTA
                     NAIGTAVLTYQWFENGFPLTDGTSGTGTVFSGSHTTNLNLSSISTGDSGAAFYCMATN
                     GVGVTATSSSATLTVEDPVIISGPNNATADYQGTTNFSVVVGGTSPFGYQWYYNGSPL
                     TDGPSPSGSGATISGSSTSNLTVSGVTYQDSGSSYYVVVTNANNNTVGSPSASLTVLD
                     PYIATQPAKQTNSAGGTATLTVVAAGTPTLTYQWYQNNNPLSDGAGPGGSAISGSGTA
                     SLTISGLNDGNAGNYYVAITGGSGYSTNSATNALTIIDPLAIVSPPKSLVERAGDHLA
                     FTIGVTGTSPTYQWQFNGTNIAGATKSSLVLTNIQTGSNGTYTVNVVNAISTNSASAT
                     LNVINSSILPLASTNIIVARIGDGAQAFSGAGNTIYFDQYTTNGVYVSTFQVPDENIG
                     QGYGTGSSGSVYGSPALLAQGGTGNDSVNSTFLTVSAGNQQFLNFSGYCEQYPFSGSD
                     VTVSGNIYSRGFASINAFGVYSLLYTNSGIYNATGHTIRSIVTLDDANFWVIGQAGSV
                     GGAKFVNISYGSGIPTIASSLADARLIQIVGGNVIFSDVSGASGAGLYACAGTPEPVI
                     GAGTTPSTLILNEGGVPNDFAFSPDGQTVYIADS"
     gene            complement(3074..3889)
                     /locus_tag="VG754_05550"
     CDS             complement(3074..3889)
                     /locus_tag="VG754_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18709.1"
                     /translation="MRAKAFTLIELLVVIAIIAILASLLLPALTRAKQKAQGIYCVNN
                     EKQLLLAWIMYYDDNNQMLVPNAGDGQAPPTGLYAPNLCWVVGNVSSPTFQYYPPAQD
                     ETNTALLSASLLGPYTKGVGVYKCPADPGNPAGTWRVRSVSMNGYMAGDGGGILSNQF
                     VLNHRVTDIVQPTSSFVFLDERASSINDGYFEVLMTTSYGSIKVQDMPANYHNEAGGF
                     SFADGHAATQKWTTGLFDTAGTVVLTGQSAPNNLDYIWLMRNTTVLISGAPLP"
     gene            complement(4265..4672)
                     /locus_tag="VG754_05555"
     CDS             complement(4265..4672)
                     /locus_tag="VG754_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HWF18710.1"
                     /translation="MPEQETLARARKDKQEGKAPSTQAGEFVREEIHHVRQGKHGPSS
                     AKQAIAIGLSRARRAGVKLPPPRKGTVSEKTRKSAQSAYRKGQSHAGRNVSPKRSRSR
                     LNVLKREGRSAASHKALSRQARSVAQKRQQRRY"
     gene            4776..5444
                     /locus_tag="VG754_05560"
     CDS             4776..5444
                     /locus_tag="VG754_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF18711.1"
                     /translation="MARLQTQLETDIEIPFAGFKLEGELILPLHTGGVVLFAHGSGSS
                     RHSPRNQFVARTLREHGVGTMLFDLLTAYEEREDRYTGHLRFNIELLAERLLGATEWF
                     HRQNPDSPIGYFGSSTGAAAALLAETSSPVPIRAIVSRGGRPDLAGDALPMVKAATLL
                     IVGGDDDVVVELNQAALAQMRCEKELRVIPGATHLFEEPGALEQVAAMAAEWFERHFA
                     NPAG"
     gene            6013..7038
                     /locus_tag="VG754_05565"
     CDS             6013..7038
                     /locus_tag="VG754_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF18712.1"
                     /translation="MMLATVILIAQGCGTFVANRIAQAPNTYPTWFAPKARVSLGISP
                     KMMTIFPSHYVDVGNPQARVYYRVIEPAQYDLRVASSNWVEHGEQQFDFTFHAKLPGK
                     PNQWSSSPRGTVVLLHGYGLAQFSMAPWALRLAEEGWRCVLVDLRGHGKSTGRRIYFG
                     TRETNDLSRLLDKMSADGQLTGPVAAMGESYGAALALRWAGVDPRVNSVVAIAPYGVL
                     SNAVLNISKDYAGWVPLGLIKSGLRKLPSVLNVPPDELNTTTVLRRNPESALFIAGAD
                     DKIAPAAEVEKLSELAKPGSRLIVVPHATHEAVTYFFKDLSAPVLAWLQQEDESGKRA
                     ERKQASR"
     gene            complement(7870..7998)
                     /locus_tag="VG754_05570"
     CDS             complement(7870..7998)
                     /locus_tag="VG754_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18713.1"
                     /translation="MKRKSDGDLCSEGAPEISQTQSVWSREMTVFVLRRLDDELAV"
     gene            8512..8847
                     /locus_tag="VG754_05575"
     CDS             8512..8847
                     /locus_tag="VG754_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18714.1"
                     /translation="MKRYRLELFTKGWFVGNFSPTLLATDAVEVAVKHYRAGESESAH
                     FHKVATELTLIVSGRVRMNGEEAGPGEIIKIDPGEAADFVPLTDATTVVVKVPCVSGD
                     KYLCKDSKP"
     gene            8844..9482
                     /locus_tag="VG754_05580"
     CDS             8844..9482
                     /locus_tag="VG754_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWF18715.1"
                     /translation="MTQRKIKAVLFDLDGVLVDATEWHYEALNRALGLFGHHIARYEH
                     LTTYNGLPTRKKLEMLSVEKKFPRGLHTLVNKIKQKYTREEILRSCTPVFEKEFMVHQ
                     LKREGYKLAVCSNSIRESVELMLRASGLWELFDCILSNEDVKQAKPDPEIYLAACERL
                     GIKPNEALIVEDAPHGLEAARRSGAVLCAVGGFNEVDYARIKRALENGSEPG"
     gene            9514..>9708
                     /locus_tag="VG754_05585"
     CDS             9514..>9708
                     /locus_tag="VG754_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HWF18716.1"
                     /translation="MKDPVMLQVIVPMAGKAARFAERGHTFPKPLFEIGNRSMIELVL
                     ENLSPPAPVQYTFICRKEHVS"
BASE COUNT         2160 a         2553 c         2750 g         2245 t
ORIGIN      
        1 gctgtcggcg atgtaaacag tctggccatc cgggctgaac gcgaaatcat ttggcacacc
       61 gccttcattg agaatcaatg tggatggcgt ggtgccagcg ccgatgaccg gctcgggtgt
      121 gccggcacaa gcataaaggc ccgccccgga tgcgccggac acatccgaaa agatgacatt
      181 gccgccgaca atctggatta accgcgcgtc tgcgaggctg gaggcaatgg tcgggatgcc
      241 ggaaccataa gagatgttca cgaatttcgc gccgccgacc gaacccgcct ggccaatgac
      301 ccagaagttc gcgtcatcca aagtgactat gctgcgaatg gtgtggcccg tggcgttgta
      361 gatgccgctg ttcgtataga gcagcgaata aaccccgaaa gcgttgatgc tggcaaaacc
      421 gcgcgagtaa atgttcccgg agacggtcac gtccgagccg gagaacgggt attgctcgca
      481 gtatcctgaa aaattcaaga attgctggtt gccggcggag accgtcagga acgtggagtt
      541 gacggagtcg ttgccggtgc ctccttgggc aagcagtgcg ggactgccgt aaacactgcc
      601 tgaactgccg gtgccataac cttggccaat gttttcatcc ggcacttgaa aggtgctgac
      661 gtaaacgccg ttggtggtgt actggtcgaa atagatcgtg ttgcccgcgc cgctgaacgc
      721 ctgcgcccca tccccgatgc gcgcaacgat gatgttggtg gatgccaggg ggaggatcga
      781 gctgttgatg acattcaacg tggccgaagc gctgttcgtg ctgatggcat tgaccacgtt
      841 caccgtgtaa gtgccgttgc tgccagtctg gatgtttgtc aacacgagcg aacttttggt
      901 cgcaccggca atattggttc cattgaactg ccactggtaa gttggcgatg tgccggtcac
      961 accaatcgta aacgcgaggt ggtcgcccgc ccgttccacc agcgacttcg gcgggctgac
     1021 aatggccaga ggatcaataa tggtcagggc attcgttgcg ctgttcgtgc tgtatccact
     1081 gcctccggtg atggcgacat aataatttcc cgcgttgccg tcgtttaggc cggaaatggt
     1141 caacgaagca gtgcctgaac ccgatatggc gctgccgccc ggcccggcgc catcactcaa
     1201 aggattgttg ttttgatacc attgataagt gagcgtgggt gtgcccgcgg caaccaccgt
     1261 caatgtcgcc gtgccgccag cgctgttcgt ctgtttggcg ggttgtgtgg cgatgtaagg
     1321 gtccaatact gtcagcgatg ccgagggact accgacggtg ttgttgttgg catttgtcac
     1381 caccacgtaa taactgctgc ccgaatcctg gtaagtcaca ccggaaaccg tcaggttgga
     1441 ggtgcttgat ccagagattg tcgcgccgga acccgaaggc gagggcccat ccgtcagcgg
     1501 actgccattg taataccatt ggtagccgaa tggcgaggtg ccgccgacaa ccacgctgaa
     1561 attggttgtg ccctgataat ctgccgtcgc attgtttggt cccgaaataa tcacggggtc
     1621 ttcaaccgtc aaggtggcgc tggaactggt ggcagtcacc cccacgccat tggtggccat
     1681 gcagtaaaaa gcggccccgc tgtcgcccgt cgaaatgctc gaaaggttca ggttggtggt
     1741 gtgagaaccg gagaaaacgg tgcctgtgcc gctggttccg tccgtcaacg ggaagccgtt
     1801 ttcaaaccat tgataagtca ggaccgctgt gccaatcgcg tttgccgtga agctcccgcc
     1861 gccacccgtc ggcgcgttta agttgaccgg ttgcagaaca aactgcgggt cgccggtgac
     1921 cgtcacgaca gtggcgttgg tgatggaact gccgatgccg gtgctgacga tgctggaata
     1981 agttcccgca ttggggtccg ccatgttcgc gatggtcagg atgctggcgg tcgccccggc
     2041 gatgttggtg ccattgaatt gccactggta gatgacggac gaactgcctg tcgccctgtt
     2101 cgtcaaggac aaggtggcgc cgaatgcgac ggtcttggtg gcgggcggcg gggttttgac
     2161 cgcaacagcg ccggtgacaa acgcccagtt cgtgccgagg cgaaagtcgt caacgatgag
     2221 gatattgggc tgagtggtcg agcggttggc caggatgaaa gtgtttggac tagtcgtctc
     2281 atccgcatcg gtgaagacga tggtgttggt gttttcgccg ggcggagcgg ctgccccaaa
     2341 ggtgttggtg ttgggattga tccagagccg gcaaatgtca ttcgtggtgg agcttgagcc
     2401 cgacgaattg gtggtaacaa tttcatacga tgccacgatg aaaacggttt cattggtgct
     2461 gtggaccgtg gagtcgtaag ccacgctggt ggttctcgca atgccgagct gatagccggc
     2521 gccgtttttc ttgaaataaa tcctcgcgtg aaaatcgctc ggctggtttc cctgatcgcc
     2581 gctgccgttg ttaaaacccc agcagtaatc gcctgtcgtg ggcagactgg cgatgtttgg
     2641 caccgaaacc agcatcgagt aatagagctt gacgccgttt tgcgtctcga agatctgcgg
     2701 actggcggtg tttatgcgca tgccggcgcc agcggcattg gtcaggacga ttgcattccc
     2761 cgtggaagcc ggcaatccgg ggtagaacag ggaaaaattc gtgagataaa ttgacggccc
     2821 tcctcccgtg ctgttgattt ccaaccaaaa atctccctgg gcattggtct ggtgccagcc
     2881 cgggctgcca gcggcgtacg ctgtgccggt gggaaacaga cccactgaat tggcgaatgg
     2941 ctcatagcag ggcaggatgg caaaagcggc tgtggcaaaa ctcaacagga agaaaacaag
     3001 gaacgatgct tttttcatac ggtggtttgg gggtttacaa ttttgtgatt gtttgtctct
     3061 ggctttcgaa agatcacggc agcggcgctc cgctgatcag cacggtggtg tttctcatca
     3121 gccagatata atccaagttg ttgggcgcgc tctgtccagt caggaccacg gtcccggctg
     3181 tgtcgaacag tccagtcgtc catttttgag tggcggcgtg tccgtccgca aaggaaaatc
     3241 ctcccgcttc gttatgataa tttgccggca tgtcctgcac cttgatggac ccataggagg
     3301 tggtcatcag gacttcgaaa tagccgtcgt tgatggaact ggcgcgctca tccagaaaaa
     3361 caaatgaact cgtgggctga acgatgtcgg tcacgcgatg gttcaggaca aattgattgg
     3421 aaagaatgcc gccgccatct cctgccatat aaccgttcat cgaaacgctg cggacacgcc
     3481 aagtgccggc cggattgcct gggtccgccg gacatttata tacgccgacg cctttcgtat
     3541 aaggaccaag gaggctcgct gacaaaagcg cagtgttggt ttcatcctgc gcgggaggat
     3601 aatattgaaa ggtgggcgaa ctgacattgc ccacaaccca gcaaaggttg ggtgcataaa
     3661 gcccggttgg cggcgcctgc ccgtccccgg catttggcac gagcatttgg ttgttgtcat
     3721 cgtagtacat tatccacgcc aacaacaact gcttctcatt gttgacgcag taaatgcctt
     3781 gggccttctg cttggccctg gtcaacgccg gcaacagcag gctggcaaga attgcgatga
     3841 tggcaataac caccagcagc tcaatcaaag tgaaagcttt ggcgcgcaaa taaaagggtt
     3901 ggtttaattc gcttccggct atctgggaaa atttgattct gattcgattg aaaatggctt
     3961 gcaaagcagg gacggcccat atgagcacgg cacgaggctg cgtctttcca agggaatcat
     4021 gtaactcttt tagctatgcg taagtttctg tcaagatatt atttcgctta gataataagt
     4081 attattgcaa gtaactcagt aactgctcca aactggctaa ttcgcggctt ggagcttgtt
     4141 ccaccggaaa agccgttttt ttgtccatat aaaaaatcta tatttaatat tactttatgt
     4201 caatagacat aatagagatt ttaattagcc tttacctgta taaaaatctc tttgcctttg
     4261 gcactcaata acgtcgctgc tggcgttttt gcgcaacact gcgcgcctgc cgggaaagtg
     4321 ccttgtggga agcagccgaa cggccttctc gtttcaagac gttcagccgg gagcgggagc
     4381 gttttggcga aacattccgc cctgcatgac tctgcccttt gcggtaagcg ctctgcgcgc
     4441 tcttgcgcgt tttttcggaa accgttcctt tacgcggcgg cggaagtttg acccccgctc
     4501 ggcgggccct cgacaatccg atggcaatcg cctgtttggc ggaactgggg ccgtgcttgc
     4561 cctggcggac gtggtgtatt tcttcccgga cgaattcacc cgcctgtgtg ctgggggctt
     4621 ttccttcctg tttgtctttg cgtgctcgtg cgagcgtttc ttgttcgggc ataactaact
     4681 cctttggatg ttgatttgtg caataaatat aaaacgattc cgcgttggcg caatggattt
     4741 cgtggggata tgtttctata actaaacgga ccattatggc gaggttgcaa acacaattgg
     4801 aaacggacat agagattccg ttcgcaggct tcaaactcga aggcgagttg atcctgccac
     4861 tgcatacggg cggggtggtc ttgttcgcgc atggcagcgg aagcagcagg cacagcccgc
     4921 gaaaccaatt tgtcgcgcgc accttgcgcg agcatggcgt cggcacaatg ttgttcgacc
     4981 tgctgactgc gtatgaggag cgcgaagacc gctacaccgg ccacttgcgt tttaacatcg
     5041 aattgctcgc tgaacgtctg ttgggcgcga cggaatggtt ccaccgccaa aatcccgatt
     5101 cgcccatcgg ttattttggt tccagcaccg gcgcggcggc ggctttgttg gcggaaacaa
     5161 gttcccccgt tccaatccgc gcaatcgtct cgcgcggcgg acgcccggac ctggctggcg
     5221 acgcgttgcc gatggtgaag gccgcaacgc tgttgatcgt gggaggcgac gacgacgtgg
     5281 tggtggaatt gaaccaggca gccttggcgc agatgcgatg cgaaaaagaa ttgcgggtca
     5341 ttcccggcgc gacgcatctc tttgaggaac ccggcgcgtt ggaacaggtc gccgcaatgg
     5401 cggccgagtg gttcgagcgt catttcgcca accctgccgg gtaatttttg ggcgctgcat
     5461 catgggaaac agggatgtcg gcaatttttt cgcttttatt catgagtccg aacctccttc
     5521 tcgctgtcaa tctccctgaa aatgaagaaa catttggcaa aatgtctcaa aacaggctaa
     5581 gcaaacagat tcatgcattt tgaagaacag tcgaataatt tggtccgacc cttggaaaac
     5641 ccggccagcg gacctgaatc atcaaatccc caatccgaaa tttcaaattt gaattccaaa
     5701 tctcaaccct cggatttaaa tctgaaatct caaaccttga atacagattt gaaatctcaa
     5761 atctcaaatt caggtctcaa atctcagatc tcaaattcag gtcaaatctc aaattcagat
     5821 ctcaaatctc aaatcttgaa ttcagatttg aaatctcaaa attcggaatt aaatctcaaa
     5881 tctcaaattt cgaattcagg tctcaaatct caaatctcaa atttgaaatt caccggctcc
     5941 tcgtccgtct cgttccccca aaagcgccgg aaattctcct tttcacaaaa atggtccaag
     6001 ccagttctct ggatgatgct ggcaaccgtg attttgatcg cgcaaggttg cggaacattc
     6061 gtcgcgaacc ggattgccca ggcgccgaac acctatccga cctggttcgc tcccaaggcc
     6121 cgcgtctcgc tcggtatcag cccgaaaatg atgaccattt ttccgtcgca ttatgttgat
     6181 gtcggcaatc cgcaagcccg ggtttattac cgcgtcatcg agcccgcgca atacgatttg
     6241 cgagtggctt cgagcaattg ggtcgagcac ggcgaacaac aattcgattt cacgtttcat
     6301 gcaaaattgc cgggcaaacc aaatcaatgg agcagttcgc cgcgcggcac ggtggtcttg
     6361 ttgcacggtt acggcctggc gcagttctca atggcgccat gggcgttgcg attggcggag
     6421 gaaggttggc gttgcgtgct ggtggatttg cgcggccatg gaaaatccac aggccggcga
     6481 atttatttcg gcacgcgcga gacaaacgac ctgagccgct tgctcgacaa aatgtccgcg
     6541 gacggccaat tgaccggacc ggtcgcggcc atgggtgaat cttacggcgc ggcattggca
     6601 ttgcgctggg cgggcgttga cccgcgcgtg aacagcgtgg ttgcgattgc cccttacgga
     6661 gtcttgtcca acgcggtgct gaacatttcc aaagattacg cgggctgggt tccgctggga
     6721 ttgatcaagt ccggcctgcg caaattgccg tccgtgctca acgtgccgcc cgacgaactg
     6781 aacaccacga ccgtgctgcg gcgcaatccg gagtccgcgt tgttcatcgc gggcgccgac
     6841 gataaaatcg ccccggcagc cgaggtcgaa aaattgtcgg agttggcaaa acccggcagc
     6901 cggttgattg tagtgccgca cgccacgcac gaagcggtca cttatttttt taaagacctc
     6961 tccgcgccgg tgctcgcatg gcttcagcaa gaagatgaat ccggcaagcg cgcagaacgc
     7021 aagcaggcaa gccggtaagg attgaaataa tagtggagca cggttatgcg ccaagcgcca
     7081 gccgcagcaa actatcgaaa tcctgagtgt tccattgctg cggctggtcc tgcggacact
     7141 ctcgcgcttc ctctccattt tatcagatga cttgcaggag atttcttgct ctcaaaaaag
     7201 gtcgcgtcgt tttcgcggag aaaaaattgg ggttccccgc gtctcggcgg tgaatctcaa
     7261 gtaattgaga gtcagacaaa tgggatgctt ccgcacagac aatgccggcc cgcattggcg
     7321 gatttcgcgc ggaaattcag ttcaaatgca gaaaaacacc cctttttccg tgtagggagc
     7381 aaactttttg tcctacacgc caaaaagtca tgttttttga aaaacgcgaa acccgcataa
     7441 acattggtca aaacggctga aaactcacgt tttcagccac atcaaaaaaa atgctgtcaa
     7501 acctaccgta ggtttcattt tttcgggcag gccatggtaa tctcgtccca tgatagttga
     7561 aaatggtaaa ttggagtgtt gggcagatgg ctcgccaagg tcgtcaacat cgctttggcc
     7621 acgcggaaac atcgatcacg ccaccgcgct ccgcaagctg gaggcttgca agaaattagc
     7681 cgggggtaag gccggaggcc gccacccccg gttgtctgtt gttcaatttt ccaccctgaa
     7741 agggtggcag aaacgcaatt ggttcgttcg ttctggcacc ccgtcagggt gcatcggttt
     7801 gtgcgacatt ttccgggggt gtcgcttcgc tcaacccccg gctaatttct tgcaaccctc
     7861 cgggttgctt cagacagcca gttcgtcatc gagacggcgc aggacgaaaa cagtcatttc
     7921 gcgcgaccag acactttgtg tctggctaat ctccggtgct ccttcggagc acaaatcgcc
     7981 gtcgcttttg cgcttcattc gtaagtggtt gaaattccgg ataatttcag ataatttcag
     8041 ataattccgc caaaaacacc cccctccccc catgcgaacc gcctcgaaag aaacattgaa
     8101 cgcccaacat cgaacgtcga acactgaatc agggaaacgt cggtccgctc tggaacattc
     8161 gatgttcagc gttcagcgtt ggttgttcga tgttttccgc attgggccaa ccgtgtcgct
     8221 tggcgtcgtt tcggggaaaa agcggcagag gactgccgca ctccaagacg ctatcgcgac
     8281 ttcccaaaca tcgtcggagt tccgcgaggt ttttgccttt gctatcctct atcctcaatc
     8341 ctttgttccg gcggcgtgtc gctgtcgctt ggagatccaa tctgtccacc gttactccat
     8401 tactccaaca ctccaatgcc cccatgctcc cattttgacc ttgaaacggt ttgtttggac
     8461 gcggatgatg gaaattcagc cgccatgggc gggtgccatc gtcttatgcc actgaaacgt
     8521 tatcgtctgg aactgttcac caaagggtgg tttgtcggga atttttcacc cacgttgctg
     8581 gccaccgacg ccgtggaggt cgccgtgaag cattatcggg cgggcgaatc tgaaagcgcc
     8641 catttccaca aagtcgccac cgaactgacg ctcatcgttt ccggccgcgt ccggatgaac
     8701 ggcgaagaag ccgggccggg cgaaatcatc aagatagacc ctggcgaggc ggccgatttc
     8761 gttcccctga ccgacgcgac gacggtggtg gtcaaagtcc cgtgcgtcag cggcgacaaa
     8821 tatctttgca aggattccaa accatgacgc aacggaaaat caaagcagtg ttgttcgatt
     8881 tggacggcgt tttggtggac gcgacggaat ggcattacga agcgttgaac cgcgcgctgg
     8941 gacttttcgg tcatcacatc gcacgctacg agcatttgac gacttacaat gggttgccca
     9001 cgcgcaagaa attggagatg ttgtcggtcg agaaaaaatt tccgcgcggc ttgcacacgc
     9061 tggtcaacaa gatcaagcag aaatacacgc gcgaggagat cttgcgcagt tgcacgccgg
     9121 tgttcgaaaa ggaattcatg gtgcatcaac tcaaacgcga aggctacaag ctggcggtct
     9181 gttccaattc catccgcgag agcgtggagt tgatgctgcg tgcgagcgga ttgtgggaat
     9241 tgtttgactg catcttgagc aacgaggacg tgaaacaggc caagccggac ccggagattt
     9301 atctggccgc ttgcgaacga ttgggcatca agccgaacga ggcgttgatc gtggaagatg
     9361 cgccgcacgg attggaagcg gcgcggcgca gcggcgcggt gctctgcgcg gtcggcggtt
     9421 tcaacgaagt cgattacgcg cgcatcaagc gggcgttgga gaatggaagt gaaccgggat
     9481 gaagaacatc gaacatccaa cgctgaacct cgaatgaaag accccgtcat gctgcaagtc
     9541 attgtgccaa tggcaggaaa agcggcgcgt tttgcggaac gcgggcatac gtttcccaag
     9601 cctttgttcg agatcggcaa ccgttcgatg attgaactcg tgttggagaa cctttcgccg
     9661 cccgcgccgg tgcaatacac cttcatctgc cgcaaggagc acgtgtcg
//
LOCUS       DASZMM010000236         6618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4759293, whole genome shotgun sequence.
ACCESSION   DASZMM010000236 DASZMM010000000
VERSION     DASZMM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4759293"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..975
                     /locus_tag="VG754_05590"
     CDS             415..975
                     /locus_tag="VG754_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWF18717.1"
                     /translation="MLTFQRNRMALAACANRLFILAFLLAGTSRCTAGSSGVSLTWNA
                     SPDTNVAGYAIYFGTNSGSYMTRIDAGTNMAGLIPSLTPQTKYYFTVTAYNNAGIESA
                     PASEIAFTVPTLLQMIAGKTAAAGVTLGFTAAAGHWYLLQAAPDLRNWTTIWQSSVAT
                     QTAWTTYQDVPQGAPLPQKFYRLVVH"
     gene            1558..2145
                     /locus_tag="VG754_05595"
     CDS             1558..2145
                     /locus_tag="VG754_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18718.1"
                     /translation="MINFAAYAAGFELFPKTGDAFVHLSPLISHLIRVDFSLTVNDPT
                     TNQGQMSSDFDKKFEESLRMLGAVSSTFMMPSEAAQELDFTFRYQGKNIAVEIEKTNR
                     EKILRDILKCHIYLHAGADFTVIVLPANYPHKLGMWNLFEFGVQRLSECETYGFGTPD
                     KLNRILLLGFKQYDAKTGEALSTMSRKWMRNEAAK"
     gene            complement(2171..3298)
                     /locus_tag="VG754_05600"
     CDS             complement(2171..3298)
                     /locus_tag="VG754_05600"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWF18719.1"
                     /translation="MNRKQLLLLLVVLLVIGGAGVVLMKRNNESWTLPDSRMGDKVLP
                     NFQLNEVAAIHIQGDTSLDLVRKNDVWRVQERADYPANFHQISDALIKVRDLKVVQSE
                     PVSASELAQVNLEAPGKGAGSGMLVEFKDGQGKVLDSLILGKMHLREGGSSPFNNGSP
                     DGRYVLLPGDPKNALLISEPLSSFVPRPEHWVSKDFIKMEKIKTISTTSANIANAWKV
                     SRETESSPWALDAAKPGETLDTNFVSTVARMLAYCTFMDVLTGPAPADSGMDQPKLVT
                     IETFDHFTYTLKIGSKMPEENYYMKVAVASDLPMEKMATNLQDKLKQEQAFAPWVYAV
                     SSGIVVPLLRDRARIMVEKKDGNASATNPDSVKAVGASAIK"
     gene            complement(3295..5169)
                     /locus_tag="VG754_05605"
     CDS             complement(3295..5169)
                     /locus_tag="VG754_05605"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HWF18720.1"
                     /translation="MKQNHFETLLYSAVGVATMFIVMMAFYIVSSAAKVRVDVTADKL
                     HTLSPGTKKILAHLDSPVTVRFYCTQGDNAMPPTLHTYAQHIEDLLAEYQKASQGKLI
                     VKKFDPKPDSEAEDSARLNGVEGQATGPFGSDKIYLGIVISRLDEKFVLPWLAPEKER
                     LLEYDISRAIARVVNGKPPVVGIMSALPVFGRASDQMMMIKGDRGVEPWAFVSELKKD
                     FILKDVPMTAPRIDDDIKVLLVVHPRDISDATQYAIDQFVLRGGKLIAFLDPHAYFDQ
                     RHDDNQPMMIMGDSAGKSSLDKLLKAWGLQLDVDKVIADTSFAGRNTQDGSVMPTLLI
                     VTRAGIDENEAATSQIDNLVIPFAGAFTGKPVEGINESVLIKSTPNSELVDSLIATSA
                     SEAILRDFKPTSVSYALAVHLTGKFKTAFPAGNPGAVDPAQLKESSGNGEVVLVADTD
                     MLNDQSCIQVRNVMGRRVVRPMNGNLNFVQSLVELFSGDDDLISARSRASMNRPFTRL
                     KDMQTKAGRQWQEKIQALETKERETDRKIKELQMHKDGSDEQKLILSSEQQTELQSYE
                     KTRTEANRDLKELRKNLRKDTDSLEFWTKVVNIAAMPALVAFSGLVLAFGKSKRRAAR
                     "
     gene            complement(5185..5934)
                     /locus_tag="VG754_05610"
     CDS             complement(5185..5934)
                     /locus_tag="VG754_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF18721.1"
                     /translation="MNSAFVNIKAIAKRELGGYFSSPVAYVFMVIFLLLTGFFTFTMD
                     GNLFQVGQASLESFFKWHPWLFLFLVPCVGMRLWAEERRVGTLELLLTKPITAWQAIV
                     GKFLASWIFIGLSLALTFPVVITVNFLGSPDNGAIAAAYLGSFLMAGTYLAISCMTSA
                     MTRNQVISFIISVVICLFLVLCGFPPVTDLLRRLDKPWLVDLVSSFSVITHFDSFKTG
                     MLDSRDVIFFLLVIGFALFTNGVIIRSHRAG"
     gene            complement(6034..>6618)
                     /locus_tag="VG754_05615"
     CDS             complement(6034..>6618)
                     /locus_tag="VG754_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF18722.1"
                     /translation="LHQSVETLSKGYRHRTCFAQSIIHDPDVLVLDEPTDGLDPNQKH
                     EIRSLIRRMGERKAIIFSTHILEEVEAVCSRAIIIDRGQVVANGTPQELKRRSEFGGS
                     VLLAARGVTSAQLTQRLSTVPNARNTSIIKEENGKVWVRVYPKAENRDGELARNVLVA
                     ANGWEVEELHTEEGRLDEVFRKITLPDTVKEKQN"
BASE COUNT         1507 a         1807 c         1736 g         1568 t
ORIGIN      
        1 cgcaacaaga tttcaccgta cgcggcagca ttgttaaaat gattttgttt gtcagcaaac
       61 aatgaaacac ctgtaagcaa agcgctgaca aagcttgctt aaaactcgaa gcattcccgg
      121 ggttattcac aaccgccacg attcatctca attgcagtta cgtttgtttc agttaaagta
      181 aaattcaacg gtctaaaaac tctccgcgct caatcccctc caatggaacg actttgatta
      241 tcactttgcg ggccacagat tcacaaggtg acgcctccgt ttgcgtaact aaaatcgttc
      301 cgcaactttt tcttcggatt acattgctgc ttgtgaataa ctttttcatc gagcctgcct
      361 gacacctcga accgccccgc aatttctgga acctcgattg ctttgttccg tggcatgttg
      421 acttttcaaa gaaaccggat ggcgctcgcg gcttgtgcca accggttgtt catcctcgcg
      481 tttctattgg caggaacgtc gcgttgcaca gccgggtcgt cgggggtttc cctcacgtgg
      541 aatgctagtc ccgacaccaa cgtggctggt tacgcgattt atttcggaac caacagcggc
      601 agttacatga cccggattga tgccggcacg aacatggccg ggctgattcc cagcctgact
      661 cctcaaacaa aatattattt taccgtcacc gcctacaaca acgccgggat cgaaagcgct
      721 ccagccagcg agattgcgtt caccgttccg acgcttttgc aaatgatcgc tggaaaaaca
      781 gccgctgccg gcgtaacgct cggtttcacg gctgcggcgg gacattggta tctgttgcaa
      841 gccgcgccgg acctgcgcaa ctggacgacc atctggcaat cgtccgtcgc cacgcaaacc
      901 gcctggacaa cttatcaaga cgttccccaa ggcgctccgt tgccgcaaaa attttaccgg
      961 ctggtggtgc attaaagggc ggtccgaaag gtttcaaatt ctcgtgggac ccgacgtaag
     1021 gaggctctca tccgatttga aatttccaat tagggcccta accactgctg gcgggtgcgc
     1081 gatgccgatt catggcggag tcacgatcca gacactcaat atgaacttcg gcaccaacat
     1141 ggcaacgctg ccgtttgcag gaaccacaac ttacgagcgg gcgcactccc tcagattgca
     1201 accggttgat aatcaacctc ggaacgactt ctccgtcatc gcgtgcgcaa atgctttcta
     1261 ttgagaaagt gtcggggatt gggacataat ttatacacat gcctcgtccc gggcatcaat
     1321 aacgagccaa agtccgtcgc ggagtagctg tttgctgcag ggccaaccgc gagggcgcga
     1381 ttggctgcgc ccaggcgggc gcgctcccct ttgaccttga aatgctttgg gcaacggcca
     1441 aagatgggga tgtatgcaca tcacaaacca acttcacatc tgtaaaaatg atgcttggat
     1501 aaatacttgg gctaatcctt ttgagaattt gtatctctac atcaacagga cgctgacgtg
     1561 ataaattttg cagcatatgc ggcgggtttc gaattattcc ctaaaactgg ggatgcattt
     1621 gtgcatttat ccccattgat aagccatctc attcgtgtgg acttttcact gactgtaaat
     1681 gaccccacca ctaatcaagg gcaaatgagc tctgacttcg acaaaaaatt tgaagaatcg
     1741 ctccgaatgc ttggtgctgt atcgtccacg tttatgatgc cttccgaagc ggcccaagaa
     1801 cttgatttca cctttagata tcaaggaaaa aacattgcag tcgaaatcga aaagacgaat
     1861 cgcgaaaaaa ttctccgcga catattgaag tgtcatatat atctccatgc tggtgccgac
     1921 tttacagtta tagttttacc cgccaattat ccccataagc tcggaatgtg gaatcttttc
     1981 gaattcggcg tgcaacgcct tagcgagtgc gaaacttatg gttttggtac gccagacaaa
     2041 ctcaatcgga ttctgctact cggttttaag caatacgacg caaaaaccgg tgaggcttta
     2101 tcaacaatga gtcgaaaatg gatgagaaat gaagcagcca aatagtatgg gcattcgcaa
     2161 taggattgcc ctatttgatc gcactcgccc caaccgcctt cactgaatcc ggattcgtcg
     2221 ccgatgcatt accatccttt ttctcgacca tgatgcgcgc gcggtcgcgg agcagcggga
     2281 cgacaatgcc ggagctgacg gcgtaaaccc atggcgcaaa tgcctgttcc tgcttgagtt
     2341 tgtcctggag attcgtcgcc atcttttcca tcggcaaatc cgacgcgacg gcgaccttca
     2401 tgtaataatt ttcttccggc atcttggaac cgatcttcag cgtataggtg aaatgatcga
     2461 aggtttcgat ggtgacgagc ttcggctggt ccatgccgct gtcagcgggg gccgggccgg
     2521 tgaggacatc catgaaggtg caataggcca gcattcttgc gactgtggag acaaaattgg
     2581 tgtcgagcgt ctcgcctggc ttggcggcat caagcgccca cggggaggat tcggtttcgc
     2641 gcgagacttt ccacgcattg gcaatgttgg ccgaggtcgt ggaaatggtt ttgatttttt
     2701 ccatcttgat gaagtccttg ctgacccagt gttccggcct cggaacgaag ctgctcaacg
     2761 gctccgagat gagcagcgcg ttttttggat cacccggcaa aaggacgtaa cggccatcgg
     2821 gcgaaccgtt gttgaacggc gatgaaccgc cctcgcgcag atgcattttg ccgagaatga
     2881 gcgagtcgag aaccttcccc tgcccgtcct tgaactcgac cagcatgccg ctgccagcgc
     2941 ccttgccggg tgcttccaaa ttcacttgcg caagctcgga cgcgctgacc ggctcggact
     3001 gcaccacctt gaggtccctg accttgatga gcgcgtcgct gatttggtgg aagttggcgg
     3061 gataatcggc gcgttcctgc acgcgccaga catcgttctt gcgaacaaga tccaaagaag
     3121 tgtcgccttg gatgtggatg gcggcgactt cattgagttg aaaattcggc agcaccttgt
     3181 cgcccatccg cgaatcgggc aacgtccacg attcgttgtt gcgcttcatc agcacaacgc
     3241 ctgcgccgcc gatgaccagc aggacaacca acaacaggag caattgtttt cggttcatct
     3301 cgccgccctg cgtttggatt tgccgaaggc cagcaccagt cccgaaaacg ccacgagcgc
     3361 cggcatggcg gcgatgttca cgactttcgt ccagaactca agcgagtcgg tgtctttgcg
     3421 cagattttta cgcaattctt tcaagtcccg gttcgcttcc gtccgggtct tctcgtaact
     3481 ctgaagttcc gtttgctgtt cggaagaaag gatgagtttt tgctcgtcac tgccgtcctt
     3541 gtgcatctgc aattctttta tcttgcggtc ggtctcgcgt tccttcgttt cgagcgcctg
     3601 gattttttcc tgccattgcc ggccggcttt cgtctgcatg tccttcaagc gtgtgaacgg
     3661 gcggttcatg ctggcgcggc tgcgcgcgct gatcaaatcg tcatcaccgg aaaaaagttc
     3721 gacgaggctc tgcacaaaat tgaggttgcc gttcatggga cggacaacgc gacggcccat
     3781 cacgttccgc acttggatgc aagattgatc gttgagcata tcggtgtcgg ccacgagcac
     3841 cacttcgccg ttgccgctgg attctttcaa ctgcgcagga tccacagcgc caggatttcc
     3901 agcggggaaa gcggtcttga attttccggt caggtgaacg gcaagcgcgt aagacacact
     3961 ggtcggtttg aagtcgcgca ggatcgcttc gctggcgctg gtggcaatga ggctgtccac
     4021 caattccgaa ttgggagtgg atttgatcag gacgctttcg ttgatgcctt caaccggttt
     4081 gccggtgaac gcgccggcaa aaggaattac cagattgtca atctggctgg tcgcggcttc
     4141 gttctcgtca atgcccgcgc gtgtcacaat caaaagtgtc ggcatcacgc tgccgtcctg
     4201 tgtgttccgg cccgcgaacg atgtgtcggc gatgactttg tccacgtcca attgcaatcc
     4261 ccatgctttg agcaatttgt cgagcgacga tttcccggcg ctgtcgccca tgatcatcat
     4321 cggctggttg tcgtcgtgcc gttggtcgaa atacgcatga ggatccagga acgcgatcaa
     4381 cttcccgccg cgcaaaacaa actggtcgat ggcatattgc gtcgcgtcgg aaatgtcgcg
     4441 cggatgcacc acaagcaaaa ccttgatgtc atcatcaatt ctcggggcgg tcatcggcac
     4501 atccttgagg atgaaatctt ttttcagttc gctgacaaat gcccatggct cgacgccgcg
     4561 gtcccctttg atcatcatca tctgatcgga cgcgcgtcca aacaccggca atgcgctcat
     4621 gatgccgacc accggcggtt tgccgttcac cacgcgcgcg atggcgcgcg aaatatcgta
     4681 ttccagcaat cgttccttct caggcgcaag ccatggcagc acaaattttt cgtccaaccg
     4741 gctgatgacg attcccaggt aaattttgtc gctgccgaac gggccggtcg cctgtccttc
     4801 aacgccgttg agccgcgcgg aatcttcagc ttccgagtcc ggcttgggat cgaatttttt
     4861 gacgataagt ttgccttgcg acgccttttg atattcggcg agcaggtctt cgatgtgttg
     4921 cgcgtaagtg tggagggtgg gcggcatggc gttgtcgccc tgcgtgcaat aaaagcggac
     4981 ggtcacgggt gaatccagat gcgcaagaat ttttttcgtg cccggcgaaa gcgtgtgcaa
     5041 cttgtccgcg gtgacgtcca cgcggacttt ggccgcgctg ctcacgatgt aaaacgccat
     5101 catcacgata aacatcgtcg ccacaccgac ggcggaatac agcagcgttt cgaaatggtt
     5161 ctgcttcatg atggctttct ttaattaacc cgcgcgatgg ctgcggatga tgacgccgtt
     5221 cgtgaacagc gcaaaaccga tgaccagcag gaaaaagatg acgtcgcgcg aatcgagcat
     5281 tcccgtcttg aacgaatcaa aatgggtgat gacgctgaac gaggaaacca aatcaacgag
     5341 ccatggcttg tcgaggcgtc tcagcaaatc ggtcactggt ggaaacccgc acagcacaag
     5401 gaacaggcag atgaccacgg agatgatgaa gctgatgact tgattgcggg tcatcgcaga
     5461 agtcatgcaa ctgattgcca gatacgtgcc ggccatcaaa aaacttccca gatacgccgc
     5521 cgcaatcgcg ccgttgtcag gcgagccgag gaaattgacc gtgatgacga caggaaaagt
     5581 aagcgcgagc gaaaggccga tgaaaatcca agaggccaaa aatttcccga caatcgcctg
     5641 ccatgcggtg atgggtttcg tcaataaaag ttccaacgtg ccgacgcgcc gttcctcggc
     5701 ccacaatctc attccgacgc acggcaccaa aaacagaaac aaccatggat gccatttgaa
     5761 gaacgattcg agactcgcct gcccgacttg aaataaattt ccgtccatcg tgaacgtgaa
     5821 aaatccggtc agcagcaaaa aaatgaccat gaagacatag gcgactggcg acgaaaagta
     5881 accgcccagt tcgcgcttgg cgatggcttt gatgttcaca aaggcggaat tcatttgaat
     5941 tatttaggtg gtagggacat cgcgctgcga tgcccattgg aacggacggc gcagcgcgcc
     6001 gtccctaccc aacatcgcag ttcgcgtctc atttcaattc tgtttttcct tcaccgtgtc
     6061 cggcaacgtg atcttgcgga acacttcatc gagccggcct tcctcggtgt gcaattcctc
     6121 gacttcccaa ccgttggcag caaccagcac atttcgggca agttcgccgt cgcggttttc
     6181 ggctttcgga taaacgcgca cccatacttt tccattttcc tctttgataa ttgaggtatt
     6241 gcgggcgttc gggacagtgg aaaggcgttg tgtcaattgc gcgctcgtga cgccccgcgc
     6301 cgcaagcaaa accgagccgc caaattccga gcggcgtttt aattcctgcg gcgtgccgtt
     6361 cgcgacgacc tgcccgcggt caatgatgat ggcgcgcgaa cagaccgctt cgacttcctc
     6421 cagaatgtgc gtcgaaaaaa tgatcgcctt gcgttcgccc atccgccgga tcaagctccg
     6481 gatttcatgc ttttgattcg ggtccaaacc gtcggtgggt tcgtccagca cgagaacatc
     6541 gggatcgtga atgatggatt gcgcgaagca ggtgcgatgg cgataaccct tggaaagcgt
     6601 ctccacgctt tgatgaag
//
LOCUS       DASZMM010000237         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_646623, whole genome shotgun sequence.
ACCESSION   DASZMM010000237 DASZMM010000000
VERSION     DASZMM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646623"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VG754_05620"
     CDS             complement(<1..539)
                     /locus_tag="VG754_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18723.1"
                     /translation="MNKTPEQLLKKLALLSTLAASSAMADPQSSGNATDNSFSVDDNF
                     RHGWHEVTAGSSVYFTSYVRRADHPEMNYAGGFLQAAYNLTSPGSDSIFRGGFQLAPE
                     IFGADVYHGYGSYVAGGTLWLRYNFVQPGWRLVPFIEGGAGLTSIDIPHRYDGKDFNF
                     NLEVGGGVRYFICERCSLNA"
     gene            complement(683..1333)
                     /gene="pdxH"
                     /locus_tag="VG754_05625"
     CDS             complement(683..1333)
                     /gene="pdxH"
                     /locus_tag="VG754_05625"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HWF18724.1"
                     /translation="MIEKQSIADLRREYAYAGLRRADLDVDPIVQFSKWLEQAVAAGL
                     HDPNAMTLSTVDAAGQPSGRIVLLKAVDQNGFSFFTNYGSRKGSELAGNPKVSLVFFW
                     PEFERQVCITGTASKVSREMSEQYFKSRPKGSRLAAWVSSQSEAIANRAVLEKKLEEL
                     IAKHPGENVPLPPYWGGYCVAPSSIEFWQGRPNRLHDRFVYVRQARGGWVLERLSP"
     gene            complement(1367..>2844)
                     /locus_tag="VG754_05630"
     CDS             complement(1367..>2844)
                     /locus_tag="VG754_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWF18725.1"
                     /translation="AGHLADFKINELQLYTEHTFAYRNYEPIWRDWGALTGEEILKLD
                     AHCRRLGIELVPNQNSFGHLRYWLAYPPLKKLAETQEAWPDQGGAFLRHPATLAPNNP
                     GTIPFLRELYDELLPHFTSNRFNVGCDETWDLGRGQSKYLCEKLGKGRVYLDFLKKIH
                     RGTSARKKQMLFWGDIILHYPELVSELPKDCVALNWGYEANHPFDKEAGIFSKSKIPF
                     YVCPGTSTWMSLIGRHDTAFANLREAALVGRKHGAMGYLNTDWGDGGHLQPLAVSYIP
                     YLLGAAVSWCAASFDEKLLVPVVNRDVLADPTGATGKAAMALGFAHRKLNYFEANTTP
                     LGAVIAAPAPRLRELFCRNGLKYYARIPAQNIQATMEELENQHSLLNCARPASASAKI
                     FVYELELAARMAIQSCKIMLWQQSLAKGRTASSRSMAKKGIAELLELNEEFSLYWPKR
                     NKGIAMRHWPCLKWRIEDYRRGVLHFPPEVAVLGKVSVTAG"
BASE COUNT          654 a          777 c          790 g          623 t
ORIGIN      
        1 gcgttcagcg agcaacgttc gcagatgaaa tagcgcacgc cgccgccgac ctccaggttg
       61 aaattgaaat ccttaccgtc atacctgtgc ggaatgtcga tggacgtaag ccccgcgccg
      121 ccttcaatga acgggaccag gcgccagccg ggttgcacga aattatatcg gagccagagc
      181 gtcccgcctg caacgtagct gccgtatcca tgataaacat ctgcgccaaa aatttcgggg
      241 gccagttgaa aaccgccgcg gaaaatgctg tccgaacctg gcgaagtcag gttgtaagcc
      301 gcctgtagaa atccgcccgc gtaattcatc tccggatgat cggcgcggcg gacatagctg
      361 gtgaaataca ccgacgagcc ggctgtgact tcgtgccagc catgacggaa attatcatcc
      421 accgaaaagg aattgtccgt ggcattgccg gatgactgcg ggtccgccat tgcgctcgaa
      481 gccgccagtg tggacaggag cgcaagtttt ttcaaaagtt gttcaggtgt cttgttcatg
      541 cgtgcgcaaa attataatat acggactttc acgcgctaca aaatattcaa ggttgggata
      601 aatgcaacct agagtttcac tccattgttt cgccccttgt gaaaggcagg atggaaaagt
      661 tggaacgcgt ccgcaagccc gtctaggggg acagccgttc caacacccag ccacctcttg
      721 cctgccgcac atacacaaac cggtcatgca agcggttcgg acggccctgc caaaattcga
      781 tgcttgaagg ggcgacgcaa tatccgcccc aatagggcgg cagcggaaca ttttcgcccg
      841 ggtgtttcgc tatcagttcc tcaagttttt tctccaggac ggcgcggttg gcgatggctt
      901 cgctctggct ggaaacccac gcggcgaggc ggctgccctt cgggcggctt ttgaaatatt
      961 gttccgacat ttcacgggaa accttgctgg ccgtcccggt gatgcacacc tgccgttcga
     1021 attcaggcca aaaaaagacg agcgaaacct tcggattgcc ggcaagttcg ctgcctttgc
     1081 ggctgccata gttggtaaag aagctgaaac cgttttgatc cacggctttc aacaggacga
     1141 tgcggccgga aggttgtccg gcggcatcca ccgtggacag cgtcatggcg ttgggatcgt
     1201 gcaatccggc tgcgacggct tgctccagcc atttggaaaa ttgcacgatg ggatcaacat
     1261 ccaaatccgc gcggcgaagg ccggcataag catactcccg gcgaagatcg gcgatggatt
     1321 gtttttcaat cattgtggtt catcatgcgc tcacaacccg cctgtttcaa cccgcggtga
     1381 cggaaacttt tccaagaacg gcgacctcgg gcgggaaatg caaaacgccg cgccgataat
     1441 cttcgatgcg ccatttcaag catggccagt gacgcatggc aatgcctttg ttgcgcttcg
     1501 gccaatacaa gctgaattcc tcgttcaact ccagcaattc ggcaattccc ttttttgcca
     1561 tgctgcggga tgaagcggtg cggcctttgg ccagcgattg ttgccacagc atgatcttgc
     1621 acgattggat ggccattcgc gcggcaagtt ccaattcata aacaaaaatt ttcgcggacg
     1681 ctgatgccgg gcgcgcgcag tttaagagag agtgctgatt ctcgagttct tccatcgtcg
     1741 cttgaatgtt ttgcgcaggg attctcgcgt aatatttcaa tccgttgcgg cagaataatt
     1801 cgcgcaaacg cggtgctggc gcggctatga cagctccgag cggcgtggtg tttgcctcaa
     1861 aataattgag cttccgatgc gcaaagccga gcgccatcgc agccttgcca gttgcgccgg
     1921 ttgggtctgc aagcacatcg cggttgacca ccggaacaag cagcttttcg tcaaacgatg
     1981 cggcgcacca actgaccgcg gcgccgagca gatatggaat gtaactgacg gccaaaggtt
     2041 ggagatgacc gccgtcgccc caatcggtgt tgagataacc catcgcgcca tgcttgcggc
     2101 ccacaagcgc ggcttcgcgc agattcgcga acgccgtgtc atgccggcca atcaggctca
     2161 tccatgtcga agtgccggga cagacgtaaa aaggaatttt ggatttggag aaaatgccgg
     2221 cttccttatc gaacggatgg ttcgcttcat aaccccaatt caatgcaacg caatctttcg
     2281 gcaattcaga aaccagttca gggtagtgaa ggatgatgtc gccccagaaa agcatctgtt
     2341 ttttgcgcgc ggaagttccg cgatgaattt ttttgagaaa atcgagatag acgcggccct
     2401 tcccaagttt ttcgcagaga tatttgctct ggccacggcc aaggtcccac gtctcatcgc
     2461 aaccgacatt gaatcgattg ctcgtgaaat gcggcagcaa ctcgtcgtaa agctcgcgca
     2521 gaaacggaat ggtgccggga ttgttcggcg ccagcgtagc gggatggcgc aagaatgcgc
     2581 cgccttggtc cggccacgct tcttgagtct ccgcgagctt cttgagcggc ggatacgcaa
     2641 gccaataacg gagatgaccg aacgaattct ggttcggcac gagttcgatg ccaagacggc
     2701 ggcaatgggc atcgagcttt agaatttcct cgccggtcaa tgcgccccaa tcgcgccaga
     2761 tcggttcgta attccgatag gcaaaagtgt gctcggtata aagttgaagc tcgttgatct
     2821 tgaaatcagc cagatgcccg gcca
//
LOCUS       DASZMM010000238         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4484046, whole genome shotgun sequence.
ACCESSION   DASZMM010000238 DASZMM010000000
VERSION     DASZMM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4484046"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VG754_05635"
     CDS             <1..202
                     /locus_tag="VG754_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18726.1"
                     /translation="MKVLLKDEETRLYLAHAGLWTDCAQDARDFAFSLHARAVARNLN
                     LKRFQILFYFPDLDYKIVVCDS"
     gene            complement(472..2370)
                     /locus_tag="VG754_05640"
     CDS             complement(472..2370)
                     /locus_tag="VG754_05640"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWF18727.1"
                     /translation="MKRCNLFDWWRVLPLFLLAFLLPASNPLWAQGTAFNYQGRLLVN
                     SNAPTGNYDMQFKLHPTATSTNQVGSTLTLSPIGVTNGLFFVTLDFGNVYNGTLLYLE
                     IGVRPTGTLSNYTILSPTQPLNPTPYSVYSENSAAFTGAISDSQLSTNVPLFDTGGKF
                     SGPVVFSNASGNFNGALSGTFNGAATGTFTGTATGNFNGTNTGTFIGNGAGVTNVNVT
                     NIVGVVQSNPNWQVMQSTSQTAVSGNNYIATNNALTTLTLPGSPGVGSVVRLSGSGAN
                     GWKAAPNTGQAINTAILGLSAGQVWTQEGTSQTWHALTSSANGMRLAAGIGSGTGTIY
                     YSGDGGMTWNPSDAPAKNWAGIACSADGMRMVAVPNGGNAYTSINAGTNWVAQSASSN
                     VFYTCVASSADGINLAAAVGSGTGPVYTSSNGGTNWMTRSVTSQNWTGIACSSNGTKI
                     VAVPSGLAQIYVSSDSGVTWTNRGPANSWSCAASSADGNKLVAAISTGNIYTSGDGGV
                     TWTARATSQAWKGVASSADGVNLAAVYSTGFIYTSSDSGRTWLQRTNGVPSGAQAWSC
                     ITSSASGSRLVAAINGGSFDFLYLSVAQTTPLTGSLSGTQYSDIELQYIGNGQWMPLT
                     FNGSFTGY"
     gene            complement(2524..3480)
                     /gene="miaA"
                     /locus_tag="VG754_05645"
     CDS             complement(2524..3480)
                     /gene="miaA"
                     /locus_tag="VG754_05645"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWF18728.1"
                     /translation="MNERELRHPPSSLFLAGPTATGKSEIALLLAEKLNGEIISVDSM
                     QVYRGLDIGTAKPDAAERKRLPHHLIDVAELSEPFDAARFVRLAQAAVREIQSRGRMP
                     IFCGGTGLYFKAFLEGLGEAPPADDALREKLAATPLPELLQELASRDPVTFDKIDRKN
                     PRRVIRALEVIRLTGKPFSSQRASWSSPILHLQPSIIYCVQREPADLHARISERVDRM
                     FKGGLVEEVKDLLSRGLAENRTAMQAIGYRQIVEHLRGEHSLPETIELVKIRTRQFAK
                     RQMTWFRRQLPVKWIECGTKENSTCIAERMIVEFQGPSQIGR"
     gene            complement(3512..4288)
                     /gene="lgt"
                     /locus_tag="VG754_05650"
     CDS             complement(3512..4288)
                     /gene="lgt"
                     /locus_tag="VG754_05650"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWF18729.1"
                     /translation="MHSIAFHLGGLTIHWYGILVAMGFLAGLWTASRRARLVGISPEQ
                     IYDLGPWLIIGGILGGRTLYVISYWHEQFAEHPFPEIFMVQHGGLVYYGGLIGGALAT
                     MAYLHWKKLPVWKMADVLAPSIALGCVFGRIGCLLNGCCYGRQCNLPWAIHFPLTHET
                     HGVGVHPTEIYDSLLNLGFYVFLAWLFRRRKFDGHVFAVYLIGYAILRSFVEYFRGDY
                     PVYYLGGWATPAQLVSMGIIVGGLLLLFLLPRPRAVAEAK"
     gene            complement(4362..5366)
                     /locus_tag="VG754_05655"
     CDS             complement(4362..5366)
                     /locus_tag="VG754_05655"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HWF18730.1"
                     /translation="MSLTARHTTRSLSLVVALAAAIGVAGLLRAQPAPSASTSPPPFP
                     TLSSPVDQFRALLVLSPAERESHLTNRPPAIRQRILAKLHEYDAMNPNDRELRLRTTQ
                     LRWYMLSLLEVPPAARATQLATVPEADRQFVKDHLAQWDLVPEDEQKEILKYEKVIEN
                     LVAVGFTNATTTPNLATAVPSNQSGLKNLDAFLNLSSEQRQQMYDSFQKFFELSDAEK
                     QKMIGVLPLAERMQMNIALQKFAHLPKVRREQCLNSFGKFSSMSESERQEFLKNAERW
                     RELSPAERQAWRNLVNRLPQRPPLPPGLALPPPLPPPMPPVSHPPLQVNIPAPTNATQ
                     "
     gene            complement(5538..6041)
                     /locus_tag="VG754_05660"
     CDS             complement(5538..6041)
                     /locus_tag="VG754_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18731.1"
                     /translation="MNEQEYQELREQSWRRRLSQTEDAALQQYLSAHPQAQEDWLGEA
                     ELNQLLEHLPEAPVASNFTARVVQAAQFEAARNPQRSWNWPAWMSVGNWLPRTAVATL
                     AVGLVLSLGYHQHQIKTRAAFAQNVVQLTGVVSASDPELMQDFEPIRQMGEAQPKADI
                     ELIALMK"
BASE COUNT         1356 a         1743 c         1757 g         1343 t
ORIGIN      
        1 tatgaaagtt ttgttgaagg acgaagaaac gcggctctac ctggcgcatg ccggactgtg
       61 gacggattgc gcgcaagacg cgagggactt tgccttcagt cttcacgcaa gggcggtcgc
      121 gagaaatctc aatctgaaaa ggttccagat ccttttttat tttcccgacc tcgattacaa
      181 aatcgtggtc tgcgattctt gatgggcgcg cggattgccg cgaccctttg cccactgcca
      241 cagcgcgaca cattccgcac aaattgccgc gcggcatcca gcatatcggg cgggatgctt
      301 tcttcgctca ggttcaaggc ccagcgaaac gccagagccg gctccaatct ttgatgtgtt
      361 tccacgcgtt gcagctcgcg gagaaatctt tcttttcgtg tcatgcaaac tccatcttga
      421 agggaagtct cgcacgacag gtcggacaaa ggctgtcctt gtgaagaagt tttagtagcc
      481 ggtgaacgag ccgttaaagg tcaacggcat ccattggccg ttgccgatgt attgcaactc
      541 gatgtccgaa tattgcgttc cggaaagcga gccggtcaac ggcgtcgttt gcgcgaccga
      601 aagatataga aaatcaaaag aaccgccgtt gatcgccgcg accaatcgcg agccgctcgc
      661 cgacgaagtg atgcacgacc acgcttgcgc cccgctcgga acaccgtttg tccgttgaag
      721 ccacgtccgg ccggaatcgc tcgacgtata aatgaaaccg gtgctgtaaa cggccgcgag
      781 attcaccccg tcagccgatg aagcgacgcc tttccacgcc tggctggtcg cgcgcgcggt
      841 ccacgtcacg ccgccatcgc ccgaggtgta gatatttccc gtgctgattg cggccacgag
      901 tttgttgccg tcggcggaag aagcggcgca actccacgaa ttggccggtc cgcgattcgt
      961 ccatgtgact cctgaatcgc tcgaaacata aatctgcgcc aagccgctcg gcaccgcgac
     1021 gatcttcgtc ccgttcgacg aacaagcaat ccccgtccaa ttttggctgg tgacacttcg
     1081 cgtcatccag ttcgttccac cgttgctcga agtatagacc ggtcccgtcc cgctgcccac
     1141 agcggcggcg agattgatcc cgtccgccga agacgcgacg cacgtgtaaa acacattgct
     1201 gctggcgctc tgggccaccc agttcgttcc cgcgttgatt gaagtataag cattcccgcc
     1261 attaggcacg gcgaccatcc tcatcccgtc cgccgagcag gcaatgcccg cccagttctt
     1321 tgccggcgcg tcgcttgggt tccaagtcat cccgccgtcg cccgaataat aaatcgtgcc
     1381 cgtgccgctt ccaatcccgg cggcaagccg catcccgttc gcggatgaag tcaacgcgtg
     1441 ccaagtttgc gatgtccctt cctgcgtcca aacctggccg gcggacaggc cgagaatcgc
     1501 ggtgttgatc gcctgcccgg tgttcggcgc cgctttccag ccattcgcgc ccgagccgga
     1561 caaccggacg acgctgccga cgcccgggct tccaggcagc gtcaacgtcg tcaacgcatt
     1621 gttcgtagcg atgtaattgt tgcccgagac cgcggtttgc gatgtggatt gcatcacctg
     1681 ccagttcgga ttgctctgca cgacgcccac gatgttcgtc acgttcacat tcgtcacgcc
     1741 cgcgccattg ccgatgaacg tgccggtgtt cgtcccgttg aaattgccgg tggccgttcc
     1801 ggtgaacgtg cctgtggccg cgccgttgaa ggttcccgac aatgcgccgt tgaaatttcc
     1861 cgatgcgttc gaaaaaacca ccgggccgga aaattttcct cccgtgtcga acagcgggac
     1921 attcgtcgaa agttgcgaat ccgaaattgc gcctgtgaac gccgcgctgt tttccgaata
     1981 gaccgagtaa ggagttggat tgagcggctg cgtcgggctg agaattgtgt aattggataa
     2041 agtgccggtc gggcgcacgc cgatttcaag gtaaagcaaa gtgccgttgt aaacatttcc
     2101 aaaatcgagc gtcacgaaga acaaaccgtt cgtcacgccg attggtgaaa gcgtcagcgt
     2161 ggaaccgacc tggttcgtgc tcgtcgccgt gggatgcagt ttgaactgca tgtcgtaatt
     2221 gcccgtgggc gcgtttgaat tcaccagcaa tcgtccttgg taattgaacg ccgttccctg
     2281 tgcccaaagc ggattggaag ccggcaacag aaaagccaaa agaaaaaggg gcaaaaccct
     2341 ccaccagtcg aacagattac aacgcttcat gattgcttac aacagccgct gttgattcaa
     2401 cattttgcat tgccgccatg gctccggccc agcgacgcaa ccgaaaaatg gcgacaatct
     2461 tcatcaaagc aggaatgccc agtcatggcg agcataaaag tgcgcatgtg ctcgttgccg
     2521 atcttatctg ccgatttggg atggaccctg gaattccaca atcattctct ctgcgatgca
     2581 cgttgaattt tctttggtgc cgcactcgat ccacttcacc ggcaactggc gacgaaacca
     2641 ggtcatctgg cgcttggcga attgacgggt gcgaattttg acaagctcaa ttgtttccgg
     2701 caacgaatgc tcgccgcgaa gatgttccac gatttggcgg tagccgatgg cctgcatcgc
     2761 agttcggttt tcagcaaggc cgcgcgataa aaggtccttc acttcttcga ccaaaccgcc
     2821 cttgaacatc cggtccacgc gttcgctgat gcgcgcgtgg agatcggcgg gctcacgttg
     2881 aacgcaataa ataattgagg gttgaaggtg gaggattggc gaagaccatg aagcgcgttg
     2941 tgaagaaaac ggtttcccgg tcaggcggat gacttcgagc gcgcggatga cgcggcgcgg
     3001 gttcttgcga tcaatcttgt cgaacgtgac gggatcgcga ctcgcgagtt cttgcagcaa
     3061 ttcgggcaag ggcgtcgcgg caagtttttc gcgcaacgca tcgtcggcag gcggagcttc
     3121 gcccaaccct tcgaggaatg ccttgaaata aaggcccgtt ccgccgcaga aaatcggcat
     3181 gcgtccgcgt gattgaattt cccggacggc cgcctgcgca agccgcacga atcgcgcggc
     3241 gtcaaacggc tcgctcaatt ccgcgacatc aatcagatga tgtggaaggc gtttgcgttc
     3301 tgccgcgtcc ggtttcgcgg tgccgatgtc caagccgcga tagacctgca tggaatcgac
     3361 ggagatgatt tcgccgttca gcttttccgc gagcaaaagc gcgatttcag attttccagt
     3421 ggctgttggg ccggcgagga agagagagga tggaggatgg cgtagttcgc gttcgttcat
     3481 tccatctcta tcgtctattt tctatcttct atcatttggc ctctgcaaca gcgcgcggtc
     3541 ggggaaggag aaaaagcagc aacaagccgc cgacgatgat gcccatgctg acgagttggg
     3601 ccggcgtcgc ccagccgccc aaataataca caggataatc gccgcggaaa tattccacga
     3661 aggagcggag aatcgcgtat ccgatcaggt aaaccgcgaa gacatggccg tcgaatttcc
     3721 ggcggcgaaa cagccacgcg aggaacacgt agaaaccaag gttcaacagc gaatcataga
     3781 tctcagtcgg atgaacacca accccgtgcg tttcgtgcgt cagcgggaaa tgaatcgccc
     3841 acgggagatt gcactgacgt ccataacagc acccgttcaa caagcagcct atgcggccga
     3901 acacgcaacc gagcgcgatg cttggcgcga ggacatcggc cattttccag accggcagtt
     3961 ttttccaatg cagataggcc atggtcgcca acgctccgcc gatgagtccg ccgtaataga
     4021 ccagtccgcc gtgctgcacc atgaagattt ccgggaatgg atgttcggcg aattgttcgt
     4081 gccaatagga aatcacatac aacgtgcgtc cgcccaggat gccgccgatg atgagccacg
     4141 ggccaagatc gtagatttgt tcgggagaaa ttcccacaag tcgcgcccgg cggctggcag
     4201 tccagagccc ggccaaaaag cccatcgcca ccagtatccc gtaccagtgt atggtcaatc
     4261 cgcccagatg aaacgcaata ctatgcattc ggtttcaaca taatgacggt tgcatggagt
     4321 gacaagtggc tttcaagccc aaaaagttgg gcgaagcccg gctactgggt ggcattcgtc
     4381 ggtgcgggaa tgttgacctg gagcggagga tgcgagactg gcggcatggg cggaggcaat
     4441 ggcggaggca gcgcaaggcc gggcggcaat ggcggacgtt gcggcaggcg gttgacgaga
     4501 ttgcgccaag cctgccgttc cgcgggggac aactcgcgcc agcgctcggc gttcttgaga
     4561 aattcctgcc gctcgctttc gctcatgctt gaaaatttgc cgaaggaatt gagacattgc
     4621 tcgcgtcgaa ccttgggcaa gtgggcgaat ttctgcagcg caatgttcat ctgcatccgt
     4681 tccgcaagcg gcagcacgcc gatcatcttc tgcttctcgg catcgctcaa ttcaaagaat
     4741 ttttggaaac tgtcatacat ttgctggcgt tgttccgacg ataaattcag gaacgcatca
     4801 agatttttca gcccgctttg atttgaggga accgccgtcg cgagattcgg agttgtggtg
     4861 gcgttggtga aacccaccgc aacgaggttt tcgatgacct tctcgtactt caaaatttcc
     4921 ttttgttcat cctcgggaac gaggtcccat tgcgcgagat ggtctttcac gaactggcga
     4981 tcagcttcgg gaacggtcgc caactgcgtg gcgcgtgcgg cgggcggaac ttccagcaag
     5041 gaaagcatat accaacggag ttgcgtggtg cggagacgca attcacggtc gttgggattc
     5101 atcgcgtcgt attcgtggag cttggcgaga atgcgctggc gaatcgcagg cgggcgattg
     5161 gtcaggtgac tttcgcgttc ggcaggcgag aggacgagca acgcgcgaaa ttgatccact
     5221 ggcgatgaca acgtcgggaa aggcggaggt gaagtgctcg ccgacggcgc cggttgcgcg
     5281 cgcaaaagac ccgcaactcc aatggcagcg gccaaagcca caaccaaaga tagactgcgt
     5341 gtcgtatgac gggccgtgag tgacattctt taagtaatgc aaggccgatg gatgcaaatg
     5401 aacgcagatt gcaaggtcac agagatcata ttcaattcat tgccagcccc aaccgctggt
     5461 gatctccatc aagtacccat aaacttgatt tgttccttct gaatctgcgt tcatctgcat
     5521 ccatcggcgg ttaaaattta tttcatcaag gcgatcaact cgatgtcggc cttcggttgc
     5581 gcttcgccca tttggcgaat cggttcaaaa tcctgcatca attccgggtc ggacgcggaa
     5641 acgacgccgg taagttggac gacattttgc gcgaacgccg cgcgggtctt gatttgatgc
     5701 tgatgataac ccagcgacag caccaaccca acggccagcg tcgcaaccgc ggttctcggc
     5761 aaccagttgc caacactcat ccaagcaggc cagttccaac ttcgctgcgg attacgagcc
     5821 gcttcaaatt gcgccgcttg gacgactcgc gcagtaaaat ttgatgcgac aggcgcttcg
     5881 ggcaaatgct ccaagagttg gttcaattcc gcttcgccca gccaatcttc ctgggcttgc
     5941 ggatgggccg acagatattg ttgcaacgcg gcgtcctcag tctgcgacag cctgcgacgc
     6001 cagctttgct cgcggagttc ttggtattcc tgctcgttca taacatcgtc gtatatgaag
     6061 gtttaaaacc ggggttttgg gaaagttccg ctcattttgc ggtttttctc cattccccgc
     6121 ttcggagata aggtttcagc ttctgtttca aagtctcgcg tccccggtgg atgagcgact
     6181 tggtggccga aagcgagca
//
LOCUS       DASZMM010000239         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1478794, whole genome shotgun sequence.
ACCESSION   DASZMM010000239 DASZMM010000000
VERSION     DASZMM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478794"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1111
                     /locus_tag="VG754_05665"
     CDS             104..1111
                     /locus_tag="VG754_05665"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HWF18732.1"
                     /translation="MNARNELLISLLHDTSRSFYRTLRVLPRGIRPQIGLAYLLARAT
                     DTIADTEIVPTEQRLDALDQLRGRILSANRQPLDFGALAKHQGSPAERTLLERIEEAL
                     DALEKFNAADQQRIQDVLKVITSGQELDVRRFAGASADNIIALANASELDDYTYRVAG
                     CVGEFWTKMCRTHLFTAARLDDKKLLADGVRFGKGLQLVNILRDLPADLRQGRCYLPS
                     DELAALGLAPKDLLDPANEPKFRPLYDRHLAVAENHLAAGWAYTNTLPRRCVRVRLAC
                     AWPILIGMSTLARLRVENVLAANRIKISRSEVKTLLLKSVIYYPFPPVWRRLFSARTG
                     A"
     gene            1181..1750
                     /locus_tag="VG754_05670"
     CDS             1181..1750
                     /locus_tag="VG754_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HWF18733.1"
                     /translation="MTKRITKQLSPKQREELLRALKARFEKNMDRHEGLDWAKVQAKL
                     EANAEKLWSLNEMERTGGEPDVVGHDKKTGEYVFYDCSAESPQGRTSVCYDREGLESR
                     KEHKPKNTAVDMAAAMGINLLTEEQYLELQKLGSFDTKTSSWVKTPADIRKLGGALYC
                     DRRYGRVFVGYNGAQSYYSGRGFRGSLRV"
     gene            1916..2626
                     /locus_tag="VG754_05675"
     CDS             1916..2626
                     /locus_tag="VG754_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18734.1"
                     /translation="MQARARELLRQKMAELNASTNSPTPAPAPAAPAKPATPPPVAAT
                     PAPTPAPPVQVAPAPPVATPAPTPVAANPSLTPDQDAKVREALRQATADAQQTTAQPA
                     PVQPVAPPPPAVQPAPAPAPVALETPQPVFRLTPEQAARVLEAERAEKARLDAEAAAN
                     ATKQKPAPVVAKQEKEMPVAPAPINVTAKSETFPAAPPSGLPASKEQRLNELLERYKA
                     DKITPSEYHQERAKILAE"
     gene            2739..>3163
                     /locus_tag="VG754_05680"
     CDS             2739..>3163
                     /locus_tag="VG754_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWF18735.1"
                     /translation="MHYFHYIGSKLYCEDVPVESLVRKHGTPLYIYSQRTLSGHFQKL
                     DHALAPVDHLICFAMKSNSNLAVLRALADLGGGFDIVSEGELRRVLAAGGDPTKCVFA
                     GVGKTEPEIEFALRHGVYSFNAESEPELVRINRVAARLK"
BASE COUNT          748 a          876 c          867 g          672 t
ORIGIN      
        1 tgttgtggtc tgtttactac gccaaaacaa accgcatttc cccggtcatc ctcgcgcatc
       61 tttataatga tgtgagcgcc actttgttct atatggcacg ccaatgaacg cacgaaacga
      121 actgctaatc agtctgctcc acgatacttc gcgctcgttt tatcggacgt tgcgcgtctt
      181 gccgcgcggg attcgaccgc agatcgggtt ggcgtatctg ctggcccgcg ccacggacac
      241 catcgccgac actgaaatcg tcccgacgga acagcgcttg gacgctttgg accaattgcg
      301 cggacggatt ttgagcgcaa accgtcagcc attggatttc ggcgcgctgg cgaaacatca
      361 aggttcgccc gccgaacgga ctttgctgga aaggatcgag gaagctctcg atgcccttga
      421 aaaatttaat gccgccgacc agcaacgcat ccaggacgtg ctcaaagtca tcaccagcgg
      481 gcaggaattg gacgtgcgcc gtttcgcggg cgcatcggcg gacaacatca tcgcgctggc
      541 caacgcatcc gaactggatg attacactta tcgcgtggcc ggttgcgtcg gggaattttg
      601 gacgaagatg tgccggacgc atctgttcac agccgcgaga ttggacgaca aaaaattgct
      661 ggcggacggc gtgcgtttcg gcaaggggct tcaactggtg aacatcctcc gcgacttgcc
      721 ggcggatttg cgacaaggcc gttgttatct gccgtccgac gaactcgcgg cgcttggcct
      781 tgcgccaaaa gatttgctgg atcccgccaa cgaaccgaaa ttccgtccgc tttatgacag
      841 gcatctggct gtcgccgaaa atcatttggc cgcaggatgg gcttatacaa acactttgcc
      901 gcgccgttgt gtgcgggtgc gattggcttg cgcatggccg attttaattg ggatgagcac
      961 gctggcaagg ctgcgcgtgg aaaacgtact ggctgcgaac cggatcaaga tcagccgttc
     1021 cgaggtgaag acgctgttgt tgaaatcggt gatttattat ccatttccac cggtctggcg
     1081 gcggttattt tccgctcgca caggcgcata ggcatggctc aaggcatgaa catggcacat
     1141 taccgattgc aaaatatcgc aagcaaattg aaataacatc atgacgaaga gaattacaaa
     1201 gcagttatca ccaaaacaac gtgaagaact gctcagggca ttgaaagccc gttttgagaa
     1261 aaacatggac cgccatgaag gtcttgattg ggctaaagtg caggcaaagc tggaagccaa
     1321 tgctgaaaaa ctgtggtcgc tcaatgaaat ggaaaggacc ggcggtgaac cggatgttgt
     1381 tggtcatgat aaaaagacgg gcgaatacgt tttttatgat tgttcagcgg aaagtcctca
     1441 aggccgcaca agtgtttgtt acgatcgtga agggcttgaa tccaggaaag aacataaacc
     1501 aaaaaatacc gccgttgata tggcagcggc catgggcatc aaccttttaa cggaagaaca
     1561 atatctggag ctgcaaaaac ttggaagttt cgatacaaag acgtcgagct gggtcaaaac
     1621 gcctgctgat attagaaaac tcggcggcgc gctttattgc gatcgccgct acggccgtgt
     1681 cttcgtgggg tacaacggag cgcagtctta ttatagcggc agggggttcc gtggctcgct
     1741 aagggtctaa attttacaga gaggagtgtt ccaagttgca gccgaaagct gttgcatttt
     1801 gcgaacgcat tcagtacaaa atgaagttat gcacttccag aagttgttgt cgttgatttg
     1861 tgtggcggcc ctcgttggcg ccagtgccca gagcgttcgt gcgacggata ctgagatgca
     1921 agcgagagcg cgcgaattat tgcgccaaaa aatggccgag ttgaacgcct cgaccaattc
     1981 tccaactcca gcaccggctc cggccgcgcc cgcaaagccc gcaactcctc cgccggttgc
     2041 tgcgactccc gccccgacac cagcgccgcc tgtccaagtc gcacctgcgc ctcccgttgc
     2101 aacacctgca ccaacgcccg ttgctgcgaa tcccagcctt acccccgatc aagatgccaa
     2161 agttcgcgaa gcgttgcgtc aggcaaccgc ggatgcccag caaaccaccg cccagcctgc
     2221 tccggtccag cctgtcgcgc ccccgcctcc cgcagtccaa cctgctcctg ctccggcgcc
     2281 cgttgccttg gaaactccgc agccggtttt caggttgacc ccggaacaag cagcaagagt
     2341 tctcgaagcg gaacgcgcgg agaaagcccg tctcgacgcc gaggctgctg ccaatgccac
     2401 taaacaaaaa cccgctccgg tagttgcgaa acaggagaaa gaaatgcccg ttgcgccggc
     2461 cccgattaac gtcaccgcga aatcagagac ctttcccgct gctcctcctt cggggcttcc
     2521 ggcttcgaaa gagcaacggc tgaacgaatt gttggagcgc tacaaagccg acaagatcac
     2581 gccgtccgag tatcatcagg aacgagcgaa gattttggcg gaataaaaag cccccatcaa
     2641 agaattcagc ggcgacgcag gaagttggca ttcggccgac tttgaacctc aaacttttaa
     2701 actttgaacg agcgaacgcg ctttggcata gttttgtgat gcattatttt cattacatag
     2761 gaagcaagct ttactgcgag gatgtgccgg tggaatcgct ggtccgcaaa catggcacac
     2821 cgctttacat ctattcccag cgcacgttga gcgggcattt ccaaaaattg gaccacgcgc
     2881 tcgcgccggt ggaccacctc atctgcttcg cgatgaagtc caattccaac cttgccgtgc
     2941 ttcgcgcgct ggcggatttg ggcggcggct ttgatatcgt gagcgaaggc gaattgcgcc
     3001 gtgtcctcgc cgcgggcggt gatccgacaa aatgcgtttt tgccggcgtc ggcaagaccg
     3061 aaccggaaat tgaatttgcc ttgcgccatg gcgtgtattc cttcaacgcg gaaagcgagc
     3121 ctgaacttgt ccgcatcaac cgcgttgccg cgcgcttgaa aaa
//
LOCUS       DASZMM010000240         9180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2010834, whole genome shotgun sequence.
ACCESSION   DASZMM010000240 DASZMM010000000
VERSION     DASZMM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010834"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1023
                     /locus_tag="VG754_05685"
     CDS             10..1023
                     /locus_tag="VG754_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWF18736.1"
                     /translation="MTSVPPVVLEEREAVERLATSRKRIEAELGKAIVGQKEVIEQIL
                     IALFAGGHCLITGAPGLAKTLLVKSIAQIFHLKFQRIQFTPDLMPADITGTEILQESA
                     DGRKMVFVRGPIFANMILADEINRTPPKTQAALLEAMQEHQVTAAGVRHALEEPFFVL
                     ATQNPIEMEGTYPLPEAQLDRFLFNVVINYLPEEAEVAVVRQTTEKRPMLIEPIFTGE
                     DVLRFHDFVRQVPIAENLVRYAVRLTAASRPQQTGSPAYINDWVSWGAGTRAAQSLVL
                     GAKARVLLHGRTHVSAEDIRTLAAPVLRHRILVNYRAEAEGVNVDTIIRKLLETVAEP
                     GSA"
     gene            1031..1996
                     /locus_tag="VG754_05690"
     CDS             1031..1996
                     /locus_tag="VG754_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWF18737.1"
                     /translation="MPASPNSNTPSSAPKNGFVDPRALMAIRNLELRARVVVEGFWNG
                     LHRSPYHGFSVEFTEYRPYSPGDDVRYLDWRLFARSDRFFIKKFEDETNLRCHLLVDQ
                     SRSMTYASVGWSKSDYARTLAATLAWFLNAQGDAVGLFTFDESVRDYLPPRHRHGHLR
                     QLMVALEHGPQGKGTNLGEPLRRLAELAHKRGLMLLISDLLAPVDELESHLARLAAAG
                     HEAAIFQVLDPNELAFEFPRAMLFQDTESEREFFLDPASVRADYQRKLQRHCEQAESI
                     CRKLGFSFHRLVTDQPLEIALLDFLRSRSRRGKLVRRHTQRSTPS"
     gene            1993..4041
                     /locus_tag="VG754_05695"
     CDS             1993..4041
                     /locus_tag="VG754_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HWF18738.1"
                     /translation="MSFLTPWFLFGALAVAGPIVFHLIRRSAGERMPFSSLMFLMPVP
                     PRATRRRKLEHLWLLLLRCLCLLLLATGFARPFFAHNQPLPDAIVDGRQIILLLDTSA
                     SMRRDGVWEKARAIAQSYLDKAAPADELAILTFDRQLRTIVNMPEWSSWSAEQRSVLA
                     RQRLAAVSPGWMGTQMGLALTGAAEQFDSTGGKSAKSRVLVLISDLQEGARLDGLQGH
                     EWPANTRVVIERVQAGNESNAGLEILEPSANATSVENDIHVRVANSSDSHKEKFHLNW
                     SGSGDAMEIYLPPGQSRTFTAPKISTNMISGKLELSGDDVDFDNAAYFVAPETEVVKI
                     VYFGADSADEPAGLRYYLQRVFQPTPRREIKFVPGESLSQSDFAIVPGKLGPEQCAAL
                     RDWLAGGKSALLVLTNAEAAPTLATLLGLPDVQVTEAAGDFALLGQVDFKHPIFAPFD
                     DPRYSDFSHIHFWKHRRWEIPPTLQASVLAKFDDESPALTQIAIGKGQLLVLASGWDP
                     ADSQLAVSSKFPPLMQTMLQWSGSSAAVHSQFRTGDAIPSPVLSGNTVQWKKPDGKMV
                     TLPAGTPFVETDMPGIYHAIAENRDRQFAVNLPLEESRIAPLAQDELARLGVPLGPIA
                     DQNIKLARVHQQQLAQSELENRQKLWRWLLAGALAITLMEIVLGGRLARRVTTVEAAT
                     "
     gene            4041..5651
                     /locus_tag="VG754_05700"
     CDS             4041..5651
                     /locus_tag="VG754_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18739.1"
                     /translation="METRLQNEIAPLELSLRRAQFWRELTKGWLATLALAISLLLLRI
                     FTGWTSPIQWLLPLFVALIALLISVQRLAARQNNLRAVVSAIEREHPEIRHLLSAAAE
                     QEPDKDSGNFRYLQLRVIDEVLQHPQRALWKRSLEQKLATARNKALTVAGIFLIALLA
                     LGYGSSRAHPMMASWPLQEITVTPGDTEVERGTTLVISARFGRPPAEATLVVVTASGK
                     TKRIPLERHLADPVFGASLSEMSEEGIYRVEYAGGKTRDYKITLFDYPALVRADASLR
                     FPEYTGMTNRTIPNTLRVSAVEGTRLTYTLQLNKPVARARWVGANDSLSLQMVSNSIA
                     LLSEFPLTNSARYALALEDAQGRTNKFPTDFVLQVLTNQRPELKLVFPRGDQRVSKLQ
                     EVQLQAEATDDFGLLKYGVGYGMAGQDPKLVELGQNGPGRTKRAFNYLIPLETLGVDV
                     DQVVAYFIWADDLGPDGQPRRTYSDIFFAEVRPFDEIFRPDQSGASDQSGAQGQQGNE
                     DVKLAELQKEIVIATWKLQREKLNPMKP"
     gene            5840..7993
                     /locus_tag="VG754_05705"
     CDS             5840..7993
                     /locus_tag="VG754_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18740.1"
                     /translation="MPQSTPTSTYTSTLRTVAESQDEALAQAREMLDQTENPVTHQAL
                     ESAIKEMERAKTALATAKDSPEKLPAAIAAEQAAYQALLKMIPRETRVSRSRRASRGG
                     SAGQAGQEQLNQLELTSEENRYETERQASAAPTEQQREQLRIADRLKELAQRQQDLNE
                     RLKELQTALTEARTEQEREEAQRQLKRLTDEEKQMLANMDELRQQLDSSPNANSLSKA
                     RQQLEQTRSDTQRAAQELENKSASQALAAGTRAQQEMEKLRSDLRSQTSSQFAEQMRQ
                     MRNQARDLAREQEEIGKAMESLNNLDHKSLEDSTPRKQLGEKMARQQSALTNLLSNMQ
                     TVSEQAESSEPLLSQQLYDTLRRENQTHTENLLDASAQMVERGLLPQAAEAEGAARTN
                     INELKQKIERAAESVLGNEGDALRYAQKELDDLANRLERELANGETNSNAAAASQGGS
                     NSSSRAHQSQSGASDTNSTADREGQQASGQNSQTGEQQAGTNQSSSGNSANSSTRDNQ
                     RNGNKNNGQQAGDNPQPGEQQSNGGQKGAGNQANNSAQNNQRNGNSNNNGQQATAGNS
                     QSGDQQQPGDASGNGSDTERLSQLARRIGANNGTGGLSYGPITGNDFVDWSSRMRDVE
                     QVLDAPNMRNQLATARERLAEFRAQFRQSRIKPKSELVRRQILDPIAEVRTQLREDLA
                     RMSNASSLVPLDHDPVPDNYSELVRKYYEKLGDGR"
     gene            7990..>9180
                     /locus_tag="VG754_05710"
     CDS             7990..>9180
                     /locus_tag="VG754_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18741.1"
                     /translation="MTGPATIVFTSHVWLWPALALAIAAAAALIWAGHRSAIEQRIRL
                     GCGLLKIAGVLTLAVCLLEPLRLGQRARPGANIFAMLADNSQSLQIKDANQSQSRGEM
                     LREQLTRDRDGWQAALSENFQVRRYAFDTRLQDMPDFGELNFSGRSSALGNALRTAAG
                     QWRGQPVAGVLLFTDGNATDIASDFSGFEDCPPVYPVVIGQDNGLHDLSLDKVAASQT
                     AFEDAPVTVQAEVGVHGFSSSTVVTRLIEVATSTTNSATNAPAATNTSLAGDSRVIAE
                     QQQSANGDDTSLNFRFQIQPEKPGLHFYELETRLRDEAGQPQAASREATLVNNRRLVV
                     VDRGQEPFRVLYVGGRPNWEFKFLNRALEEDPQVQMVSLLRMARREPKFEFKGKSGES
                     SNPLF"
BASE COUNT         2094 a         2638 c         2565 g         1883 t
ORIGIN      
        1 accaatccaa tcacctccgt tccgcccgtc gtcctggaag aacgcgaagc cgtcgaacgc
       61 ctggccacaa gccgcaagcg gattgaagcc gaactcggca aggccatcgt cgggcagaag
      121 gaagtgatcg agcaaatcct catcgcattg ttcgcgggcg gccattgtct catcactggc
      181 gcgccaggat tggccaagac gctcctggtc aaatccatcg cgcaaatttt ccatctcaaa
      241 ttccaacgca tccagttcac gccggacctc atgcctgccg acatcaccgg cacggaaatt
      301 ctccaggaaa gcgccgatgg cagaaagatg gttttcgttc gcggcccgat tttcgccaac
      361 atgatccttg ccgacgaaat caatcgcacg ccgcccaaaa cccaagccgc gctgctcgaa
      421 gcgatgcagg agcatcaggt caccgccgcc ggtgtccggc acgcgctcga agaaccgttc
      481 ttcgtgctcg ccacgcaaaa tccgattgaa atggaaggca cttatccgct gcccgaagct
      541 caactcgacc gctttctctt caacgtcgtc atcaattatc tgcccgagga ggccgaagtc
      601 gccgtcgtgc ggcagaccac ggaaaaacgt cccatgctta tcgagccgat ttttacgggt
      661 gaagatgtgt tgcgttttca cgatttcgtc cggcaagtgc cgattgcgga aaatttggtt
      721 cgctacgcgg tgcgcttgac cgccgcttcg cgtccgcaac agaccggctc gcccgcatac
      781 atcaacgatt gggtcagttg gggcgctggc acgcgcgcgg cgcaatcgct tgtgctcgga
      841 gcgaaggccc gcgttttatt gcatggccgc actcatgttt ccgccgagga catccgcacg
      901 cttgccgcgc cggtgctgcg tcatcgcatc ctcgtcaact atcgcgctga ggccgaaggc
      961 gtgaatgtcg ataccatcat ccgcaagctt ttggaaaccg ttgccgaacc tggttcagca
     1021 tgattcgttt atgcccgcct cgccaaattc caacacgcct tcttccgcgc cgaaaaacgg
     1081 gttcgtggac ccgcgcgctt tgatggcaat tcgcaatctt gagttgcgcg cgcgcgtcgt
     1141 ggtggaaggt ttttggaacg gtttgcatcg cagtccctat cacggtttct ctgtcgaatt
     1201 caccgaatac cgcccttact ccccgggcga cgatgtccgt tatcttgact ggcgtttgtt
     1261 cgcgcggtca gaccgtttct tcatcaagaa attcgaggac gaaaccaatt tgcgctgcca
     1321 tctcttggtg gaccaaagcc gctcgatgac ctacgcctcc gttggctggt cgaaatccga
     1381 ttacgcccgc acgcttgccg cgacactcgc atggtttttg aacgcgcaag gcgatgcggt
     1441 cggattgttc acttttgatg aaagcgtgcg tgattatctt ccaccgcgtc atcgtcacgg
     1501 ccatttgcgc cagctcatgg tggcattgga gcatggtcct caaggcaaag gcacgaacct
     1561 tggcgaaccg cttcggcggc tggcggaact ggcgcacaaa cgcggattga tgctgcttat
     1621 ctcggatttg ctcgcgcctg tggatgaatt ggaaagtcat cttgcccggc tcgccgctgc
     1681 cggacacgaa gccgccattt tccaagtgct cgacccaaat gaattggcgt tcgaatttcc
     1741 acgcgcgatg ctttttcaag acaccgaaag tgagcgggaa ttctttctcg acccggcaag
     1801 tgtgcgggcc gattaccaac gcaaattgca gcgccattgc gagcaggcag aaagcatctg
     1861 ccgcaaactc ggcttttcgt ttcataggct cgtgaccgac caaccgctcg aaattgcctt
     1921 gctggatttt cttcgcagtc gcagccgtcg cggcaagctg gttcgccgtc acacccaacg
     1981 cagcacgccg tcatgagctt tctgacgcca tggtttttgt tcggcgcgct cgcggtcgcg
     2041 ggaccgattg tgtttcatct catccgccgc tcggcggggg aacggatgcc gttcagttcg
     2101 ctgatgttcc tcatgccagt cccaccgcgc gccacgcgcc gccgcaaact ggagcatctc
     2161 tggcttctgt tgctgcgttg tctttgtctg cttttgctgg cgaccggttt tgcgcgtccc
     2221 ttttttgcgc ataatcagcc attgccagac gccatcgtcg atggccgcca aatcatcctc
     2281 ctgctcgaca ccagcgccag catgaggcgc gatggtgttt gggaaaaagc gcgcgccatt
     2341 gcgcaaagtt atttggacaa agccgcgcct gccgacgaat tggccattct gacgtttgac
     2401 cggcagcttc gcacaatcgt caacatgccg gaatggtcgt catggagcgc ggagcaacgg
     2461 tccgtgttgg cccgccagag attggctgcg gtttcgccag gttggatggg aacacaaatg
     2521 ggactcgcgc tgacaggcgc ggcggaacaa ttcgattcca ctggcggcaa gagcgcgaaa
     2581 agccgcgtct tggttttgat cagcgatttg caggaaggcg cgaggctcga cggattgcaa
     2641 ggacacgaat ggcctgccaa cacgcgcgtg gtcatcgaac gagttcaggc cgggaacgaa
     2701 tcgaatgccg gtttggaaat tttggaacct tcggccaatg ccacaagcgt cgaaaatgac
     2761 atccatgttc gcgtcgccaa ttccagcgat tctcacaagg aaaaatttca tttgaattgg
     2821 agtggaagcg gtgacgcgat ggaaatctat ctgccgcccg gccagagccg cacgttcact
     2881 gcgccaaaaa tttccactaa catgataagt ggaaaattgg aattgtccgg tgacgatgtc
     2941 gatttcgaca acgctgcgta tttcgtcgcg ccggaaacgg aagtggtgaa gatcgtctat
     3001 ttcggcgcgg attccgccga tgagccagcc ggattgcgtt attatcttca acgtgttttt
     3061 caacccacgc cgcggcgcga aatcaagttc gttcccggcg aatcgctgtc gcaatcagat
     3121 ttcgccattg tcccgggcaa acttggtccc gaacagtgcg ccgcgctgcg cgattggctg
     3181 gctggcggaa aatctgcgtt gctcgtgctg accaatgccg aagccgcgcc gactttggca
     3241 acgctgcttg gtttgcccga tgtgcaagtc accgaggccg ccggtgattt cgccctgctc
     3301 ggccaggttg atttcaagca tcccattttc gcgccgtttg atgacccgcg ctacagtgat
     3361 ttttcgcaca tccatttttg gaaacatcgc cgttgggaaa ttccgccgac acttcaagca
     3421 agtgtgctgg caaaattcga cgacgagtcc cctgccctca cgcaaatcgc catcggcaaa
     3481 ggccaattgc tcgtgctcgc ttctggctgg gacccggcag acagccagct tgccgtatcg
     3541 agcaaatttc cgccattgat gcaaacgatg ctgcaatgga gcggctcaag cgcggcggtg
     3601 cattctcaat ttcgcaccgg cgatgcaatt ccgtcgcctg tcttatccgg caacacggtt
     3661 caatggaaaa aacccgatgg aaaaatggtg acattgcccg ccggcacgcc attcgtggaa
     3721 accgacatgc cgggaattta tcatgccatt gcggaaaatc gcgatcggca gttcgctgtg
     3781 aacctgccgt tggaagaaag ccgtatcgcg ccgctcgcgc aggatgaact cgcgcgtctc
     3841 ggcgttccgc tgggacccat tgcggaccaa aacatcaagc ttgcgcgtgt tcatcagcag
     3901 caattggcgc aatcagaatt ggaaaaccgc cagaaacttt ggcgttggtt gctggccggt
     3961 gcgctggcga tcacgctgat ggaaattgtc ctcggcggcc ggctcgcgcg gcgcgtcacc
     4021 acagtggagg ctgcaacctg atggaaacgc gtctccaaaa cgaaatcgcg ccgctggagc
     4081 tttcgcttcg gcgagcgcaa ttttggcgtg aattgacaaa aggctggctt gccacgctgg
     4141 ccctggcaat ttccctcttg ctgcttcgca tctttaccgg gtggacgtcg ccgattcaat
     4201 ggctgctgcc gttgttcgtg gctcttatag ctctgctcat cagtgttcag cgtctcgctg
     4261 cgcggcaaaa caacttgcgt gcagtggttt cagcaatcga acgcgaacat cctgaaatcc
     4321 gccatctgct ttccgccgcc gccgagcagg aacccgacaa ggactcggga aatttcaggt
     4381 atctgcaact gcgggtgatt gacgaggtgc tgcaacatcc gcaacgagcg ctttggaaac
     4441 gcagccttga acaaaaactc gccacggcaa gaaataaagc tctcactgtg gcgggcatat
     4501 tcttgatcgc tttgctcgct cttggttacg gctcgtcgcg cgctcatcca atgatggctt
     4561 catggccgct tcaggaaatc accgttacgc ccggtgacac cgaagttgaa cgcggaacaa
     4621 cgctggtgat ttcggcgcga tttggccgcc cgcccgcaga agcgacgttg gtggtcgtca
     4681 ccgcttccgg caaaacaaaa cgcatcccgc tggaacggca tttggcggat cccgttttcg
     4741 gcgcaagcct gagcgaaatg agcgaggaag gaatttatcg ggtcgaatat gccgggggaa
     4801 agacgcgcga ttataaaatc actcttttcg attatcccgc gttggtgcgc gctgatgcgt
     4861 cattgcgttt ccccgaatac accggcatga ccaatcgcac aattccgaac acactccgcg
     4921 tcagcgccgt cgaaggcacg cggctgacct acacactgca actcaacaaa cctgtcgcgc
     4981 gcgcgagatg ggtgggcgcg aatgattcac tgtccttgca gatggtcagc aattcgatcg
     5041 cgctgctgag tgaatttcca ttgaccaact ccgcgcgtta cgcgctcgca ttggaagacg
     5101 cgcaaggccg cacgaacaag ttcccgacgg atttcgttct acaggtgctc acgaaccaaa
     5161 ggccggaatt gaagctggtt ttcccgcgcg gcgaccagcg cgtttcgaaa cttcaagagg
     5221 tgcaattgca ggcggaagcg accgatgatt tcgggctgct gaaatatggc gttggttacg
     5281 gcatggcggg acaggacccc aaactggttg aactcggcca gaacgggcct ggacgaacga
     5341 agcgcgcatt taattatttg attccgctgg aaactctcgg tgtggacgtg gaccaggtgg
     5401 tggcctattt catttgggcg gacgatttgg gaccggacgg acagccgcgc cgcacttata
     5461 gcgatatttt tttcgccgag gtgcggccct ttgacgaaat ttttcgtccc gatcaatctg
     5521 gcgcatctga tcaaagcgga gcccagggac aacaagggaa cgaagatgtg aaactcgcgg
     5581 aactgcaaaa ggaaatcgtc atcgccacgt ggaaattgca aagggaaaaa cttaacccaa
     5641 tgaagccatg aatgcgcatt ttcaaaaaca ccaagcacca ggcaccaagc tccagagaag
     5701 ctccaagcac aaagttccgc aggatgcgca acacaacaag tttggtgctt ggttattaaa
     5761 gattctctgg aggttggatg ttggcgcttg gagctttttc ataattattt ttttcgcgaa
     5821 ctttgcgatt gctcaggaat tgcctcaaag caccccaacc agcacttaca catccactct
     5881 ccgcactgtc gccgagtcgc aggatgaagc gcttgcgcaa gcgcgcgaaa tgctggacca
     5941 aaccgaaaat ccagtcacgc atcaggcgtt ggaatcggcc atcaaggaaa tggagcgtgc
     6001 caaaaccgca ttagccactg caaaagattc gcctgagaaa ttaccggcag ccattgccgc
     6061 tgaacaggcc gcttaccagg cactcctgaa aatgattccg cgcgaaaccc gggtgtcgcg
     6121 ctcgcgccgg gcaagccggg gcggcagtgc gggccaggcc ggacaggaac agttgaatca
     6181 gcttgaattg acgagcgagg agaaccgtta tgaaacggaa cgccaggctt ccgccgcccc
     6241 gacggaacaa caacgcgagc agttgcgcat cgccgatcgt ctcaaggaac tggcgcagcg
     6301 ccagcaggac ttgaacgagc gtttgaaaga attgcaaacc gccctgaccg aagcccgcac
     6361 ggaacaggaa cgcgaggaag cgcaacgcca gctcaagcgt ctcactgatg aggaaaagca
     6421 aatgctcgcg aacatggatg aattgcgcca gcagcttgat tcttcgccga atgcgaattc
     6481 gctctccaag gcgcggcagc agttggaaca gacgcgttcg gacacgcagc gggccgcgca
     6541 ggaattggag aacaaatcag cttcgcaagc gctcgccgcg gggacgcggg cgcagcagga
     6601 gatggaaaaa ttgaggagtg atttgcgcag ccagacttcg agccagttcg ccgagcagat
     6661 gcgccagatg cgcaaccagg cgcgcgacct tgcgcgtgaa caggaagaaa tcggcaaggc
     6721 aatggaatcg ctcaacaacc ttgatcacaa atcactcgaa gattccacgc cacgcaagca
     6781 gttgggcgaa aaaatggcgc ggcagcaaag cgccttgacc aatcttcttt cgaacatgca
     6841 gaccgtcagc gaacaggctg aaagcagcga gccgttattg tctcaacaac tttatgacac
     6901 actcaggcgc gaaaaccaga cgcacaccga aaacctgctc gatgccagcg cgcagatggt
     6961 tgaacgcggt ttgcttcccc aagccgcgga agccgagggt gccgcgcgga cgaacatcaa
     7021 tgaattgaag caaaaaattg agcgcgctgc ggaaagtgtt ctcggcaatg aaggcgatgc
     7081 tttgcgttat gcgcaaaaag aactcgatga tctcgccaat cgtctcgaac gtgaattggc
     7141 gaacggagaa accaattcca atgcagcggc tgcatcccaa ggaggcagta attcctcgtc
     7201 gcgcgcgcat caatcacagt cgggagcatc cgacaccaat tcgacggctg atcgagaggg
     7261 tcaacaggct tccggccaaa actctcaaac cggagagcaa caggcaggaa ccaatcaatc
     7321 aagttcaggg aatagcgcga acagttccac ccgcgacaat cagcgtaatg gcaataaaaa
     7381 taatgggcag caagctggtg ataatcccca acccggagaa caacaatcaa atggcggtca
     7441 aaaaggcgca ggcaatcagg cgaacaattc cgcgcaaaat aaccaacgca acggcaacag
     7501 taataataat ggacagcaag caaccgccgg gaattcccaa tcgggcgacc aacaacaacc
     7561 cggtgatgcc agtggaaatg gcagcgacac cgagcgtttg agtcagcttg cccggcggat
     7621 tggcgcgaac aacggcacag gcgggctgag ttatggaccg atcaccggga atgattttgt
     7681 ggactggtcg agccggatgc gcgatgtgga acaagtgctg gatgcgccga acatgcgcaa
     7741 tcaactggcc accgcgcgcg aacgcctcgc cgaatttcgc gctcaattcc gtcaaagccg
     7801 catcaaaccc aaatctgaac ttgtgcgccg ccagattttg gatcccatcg cggaagtccg
     7861 gacgcaattg cgcgaagacc tcgcgcgtat gtccaatgcc agcagccttg tgccgctgga
     7921 ccacgatccc gttcccgata attattccga actggtccga aaatattacg aaaaattggg
     7981 ggatggacgg tgacggggcc ggcgaccatc gttttcacct cgcacgtctg gctgtggccg
     8041 gccttggcgc tcgcaattgc tgcagcagcg gcattgattt gggcaggaca tcgcagcgcg
     8101 atcgaacagc gcattcgtct tggttgtggt ctgctcaaaa ttgccggtgt gctgactttg
     8161 gcagtttgtc tcctggaacc gttgcgtctt gggcaacgcg cgcgtccggg cgcaaatatt
     8221 tttgcaatgc ttgccgacaa cagccagagc ctccaaatca aggacgccaa ccaatcgcaa
     8281 tcgcgcggcg aaatgttgcg cgagcaattg acccgcgacc gcgatggctg gcaggcggcg
     8341 ttgtcggaaa atttccaggt gcgccgttat gcgttcgaca ctcgtttgca agacatgccc
     8401 gatttcggcg aactgaattt ttccggtcgc tcctccgctt tgggcaatgc cttgcgaact
     8461 gcggccgggc aatggcgagg acagcccgtc gcgggtgttt tgcttttcac ggacggcaac
     8521 gccacggaca ttgcttcgga tttttccggg tttgaggatt gtccgccagt ttatcccgtg
     8581 gtcatcgggc aggacaacgg attgcatgat ctctcgctag acaaagtggc cgccagccag
     8641 acagcatttg aagacgcgcc ggtgacggtg caggcggaag tcggcgtcca cggattttcc
     8701 agctcaacgg tcgtgacacg cttgatcgaa gtcgcaacca gcacaacaaa ctctgcaacc
     8761 aacgcgcctg ccgcgacgaa cacttcgctt gccggcgact ctcgtgtcat tgccgagcag
     8821 caacaaagcg cgaacggcga cgataccagt ttaaattttc gttttcagat ccagcccgaa
     8881 aagcccggcc tgcattttta cgagttggaa acgcggctgc gcgatgaagc aggccagccg
     8941 caggccgcct ctcgcgaagc cacgctcgtc aataatcgca ggctcgtggt cgttgaccgt
     9001 gggcaggagc cttttcgcgt tctctacgtc ggcggacgac cgaattggga attcaaattt
     9061 ctcaatcgcg cgcttgaaga agacccgcag gtgcagatgg tttcgctcct ccgcatggcg
     9121 cgtcgcgaac cgaagttcga gttcaaggga aaatccggcg agtcgagcaa tccactcttc
//
LOCUS       DASZMM010000241         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_909150, whole genome shotgun sequence.
ACCESSION   DASZMM010000241 DASZMM010000000
VERSION     DASZMM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909150"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="VG754_05715"
     CDS             <1..1326
                     /locus_tag="VG754_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18742.1"
                     /translation="TEIVSPFFYSEQRDTQRQWAFPPLMVHTEDSATETSGFQFIYPV
                     LTYHQYGKEHRWQFFQILSLTGGQNQTNMNTRRFTLFPLYFQQRSTDPSKNYTAFVPF
                     YGHLENRLFRDEIDFVLFPMYSKTRKKDVVNYNMPYPFFNKHYGDGMYGWQVWPFIGR
                     EHKDVTTRTNSMHEQEVNPGHESEFIMWPFYTQSTNGIGTTNASWQEGLLPLRTSYRS
                     GLRDSDSWGWPLGLTHTIDREKKYVEWDAPWPLVEFAHGEGKTERRVWPFFSRAHNEN
                     LEDVWYCWPVYKRNRIHSPPLERERTRIMFFLYSDLKMKSTETGREMRRRDFFPFFSR
                     TRDLNGNERLQVLAILEPIYSGNKGMESEYAPVYSFWRAEKNPKTGAASQSLLWDLYR
                     RDTSPQNKKISLLFGLLQYQSTPAGKRWRVCYIPMGKSRAPAGEKTSGH"
     gene            1342..2094
                     /locus_tag="VG754_05720"
     CDS             1342..2094
                     /locus_tag="VG754_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF18743.1"
                     /translation="MFQNIGEIVLLFMRTLGALPCVWRQRQKVFDQFFEIGNASLLMV
                     CILSFFIGGVLALLGGPVLAQNGLVNSVGGLVGIYMCKELAPVMMSILIAGRIGSAMT
                     AEIGSMRVYQEIDALRTMNINPIHYLVLPRMVSIAVALPLLVIFSILVGWSGGALVSA
                     SNHEIGVSFQAFFENLREMVEFSDVINGLFKSFIFALIIGVVSCHQGLQTIGGPRGIG
                     RSVTKSVVNCMVLIVIVDYFLTRMWLYIKPVS"
     gene            2091..2882
                     /locus_tag="VG754_05725"
     CDS             2091..2882
                     /locus_tag="VG754_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF18744.1"
                     /translation="MNTPPKNPHQTGVSVKVCGLHKSFNGHEVLRGLDFEVKPGEIFV
                     IMGPSGSGKTILLKHLIGLETPDVGEILINGESIQNTDLMEKYRLAMVFQSGGLLNSL
                     TVGENVGLYLSEHRLKPPAEIARIVAEKLAIVGLKGQEDKLPSELSGGMKKRVAIARA
                     LVMEPQLILYDEPTSELDPVSAVVIADEIVNLQSQIAVTSVAVSHDRELAFGIADRIA
                     MINEGRILAVGTPEEIRRNPDPVIQQFLTADYKQAKSKHEYEKHH"
     gene            2866..>3590
                     /locus_tag="VG754_05730"
     CDS             2866..>3590
                     /locus_tag="VG754_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWF18745.1"
                     /translation="MKNTIESRLGIFLFLAVVAAILIMEIIGPDLFRPGYRVRARFGN
                     IHELKVGDPVKMAGVPVGRVEKLSLATNESKVEVELRLNKNTPVHTDSKATVKSAGLT
                     GQNYVSLDFGSPTAPLLEPNELLATTEQPDLNDMMAKLDQAAAGVENLTKSFTGDKID
                     NLLGPFTDFMRQNNPRLTAIIGNFQNISSQIAKGEGTVGKLIYDQSLYNSATAAITNL
                     QDTANEIKSTVAQAHGIIDQVNAG"
BASE COUNT          807 a         1018 c          983 g          782 t
ORIGIN      
        1 acggaaatcg tcagcccatt tttttattcc gagcaaaggg acacgcaacg gcaatgggcc
       61 ttcccaccgt taatggtgca cacggaagat tccgcgacgg aaacgagcgg attccagttt
      121 atttatccgg tcctgaccta tcatcagtat ggaaaggaac atcgctggca gtttttccag
      181 atactcagtc ttaccggcgg gcaaaaccag accaacatga acacccggcg gttcacgctc
      241 tttccgcttt actttcagca gcgctcgacc gacccgtcga aaaattacac cgcattcgtt
      301 ccattttacg ggcatctgga gaaccggctg tttcgcgacg aaattgattt tgtcctgttc
      361 cccatgtatt ccaagacgcg caagaaagac gtggtgaact acaacatgcc gtatccgttt
      421 ttcaacaaac attacggcga tggaatgtac ggatggcagg tgtggccttt catcgggcgt
      481 gagcacaagg acgtcaccac gcggaccaat tcgatgcacg aacaggaagt gaatcccggc
      541 cacgaaagcg agttcatcat gtggccgttt tacacgcagt ccaccaatgg catcggcacc
      601 acgaacgcgt cctggcagga aggattgctt ccgctgcgga cttcgtaccg gtccgggttg
      661 cgcgattcgg acagttgggg ctggccgttg ggtctgacgc acacgattga tcgcgaaaaa
      721 aaatacgtgg aatgggacgc gccatggccg ctggtggagt tcgcgcacgg cgaaggcaag
      781 accgagcggc gcgtctggcc ctttttcagc cgtgcccaca atgaaaacct tgaggacgtt
      841 tggtattgct ggccggttta caaacgcaac cgcatccatt ccccgccatt ggaacgcgag
      901 cggacgcgca tcatgttttt tctctactcg gacttgaaga tgaagagcac cgaaaccggt
      961 cgcgagatgc ggcgcaggga ttttttcccg ttcttctcgc gcacccgcga tttgaacggg
     1021 aacgagcgcc tgcaagtcct tgccatcctc gagccgattt actccggcaa caagggcatg
     1081 gaatcggaat acgcgccggt gtattcgttc tggcgcgccg agaaaaatcc gaagaccggc
     1141 gcggcaagcc agtcgctgct gtgggacctt taccggcgcg acacttcacc ccaaaacaaa
     1201 aaaatctcgc tcctgttcgg gcttttgcag tatcaatcca ctccggcagg caagcgctgg
     1261 cgcgtgtgtt atattccgat gggcaaatcg cgggctcctg ccggggaaaa gacttccggg
     1321 cattgatggt gaattgattt tatgtttcag aacattggcg aaattgtgct cctgttcatg
     1381 aggacgttgg gcgccctgcc atgcgtgtgg cggcagcgcc agaaagtctt cgatcaattt
     1441 ttcgagatcg gaaacgccag cctgctcatg gtttgcatcc tgtcgttttt catcggcggc
     1501 gtgctggcct tgctgggcgg acctgtgctg gcgcaaaacg ggctggtcaa ttccgtgggc
     1561 ggccttgtcg gcatctacat gtgcaaggaa ctcgccccgg tgatgatgtc catcctcatc
     1621 gcgggccgca tcgggtcggc gatgaccgcc gagattggtt cgatgcgcgt ctatcaggaa
     1681 attgacgcgt tgcgaacgat gaacatcaac ccgatccatt acctggtgct gccgcgcatg
     1741 gtgtccatcg cggtggcatt gccgttgctg gtgatatttt caatcctggt cggctggagc
     1801 ggcggcgcgc tggtttcggc cagcaatcac gagatcggcg tttccttcca ggcgtttttt
     1861 gagaacttgc gcgagatggt ggaattctcc gatgtcatca acggcctctt caagagcttt
     1921 atttttgctt tgatcatcgg cgtggtttcc tgccatcagg gattgcagac catcggcggg
     1981 ccgcgcggca tcggccgctc ggtgacgaag tctgtcgtca actgcatggt gctcatcgtc
     2041 atcgttgatt atttcctcac ccgcatgtgg ctttacatca agcctgtttc atgaacaccc
     2101 cgcccaaaaa tccccatcag accggcgtca gcgtgaaagt gtgcggcttg cacaaaagtt
     2161 tcaacgggca cgaagtgctt cgcggccttg atttcgaggt gaagcctggc gagattttcg
     2221 tcatcatggg cccaagcggc agcggcaaaa ccattttgct caagcacctt atcgggcttg
     2281 aaacacccga cgtgggcgaa atcctcatca atggcgaatc catccagaac actgatttga
     2341 tggaaaaata ccggctcgcg atggtgtttc aatccggcgg cctgctgaat tcactgacgg
     2401 tcggcgaaaa cgtcggtctt tatctcagcg aacatcggct gaaacctccc gccgagatcg
     2461 cccgcatcgt cgcggaaaaa cttgccatcg tcgggctcaa gggccaagag gacaaattgc
     2521 cgagcgaatt gtccggcggc atgaaaaaac gcgtcgccat cgcccgcgca ttggtgatgg
     2581 agccgcagtt gattttatac gatgaaccga ccagcgaact ggaccctgtt tccgccgttg
     2641 tgatcgccga tgaaattgtg aatttgcaaa gccagatcgc cgtcacatcc gttgctgtct
     2701 cgcatgatcg tgaacttgcc ttcggcatcg ccgaccgcat cgcgatgatc aacgaaggcc
     2761 gcatcctggc cgtgggcacg ccggaagaaa tccgccgcaa tcccgacccg gtgatccagc
     2821 agtttttgac cgccgattat aagcaagcaa aatccaaaca cgaatatgaa aaacaccatt
     2881 gagagccgac ttggcatttt tctttttctc gccgtggttg ccgcaatcct catcatggag
     2941 atcatcgggc cggatctgtt ccgtcccggc taccgggtgc gcgcgcgatt cgggaacatc
     3001 catgaactca aggtcggcga tccggtgaag atggccggtg tgccggtcgg tcgcgtggaa
     3061 aaactgtcgc ttgccacgaa cgaaagcaaa gtggaggtgg aattgcgcct gaacaagaac
     3121 acgccggtcc acaccgacag caaagccacc gtcaaatccg ccgggctgac cggccagaat
     3181 tatgtttcac tcgacttcgg cagtcccacc gcgccgttgt tggagcccaa cgaattgctt
     3241 gccaccactg aacaaccgga tttgaacgac atgatggcaa agctagacca ggccgcagcc
     3301 ggagtggaaa accttaccaa gagtttcacc ggcgacaaga ttgacaacct gctcgggccg
     3361 ttcacggatt tcatgcgcca aaataatccg cgcctcaccg ccatcatcgg caatttccag
     3421 aatatttcct cgcagatcgc gaagggcgaa gggacggtcg gcaagctcat ttacgaccag
     3481 agcctttaca acagcgccac ggcagccatc accaatcttc aggacacggc caacgagatc
     3541 aaatccactg tggcgcaggc ccatggcatc attgaccagg tcaacgccgg
//
LOCUS       DASZMM010000242         5556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5032173, whole genome shotgun sequence.
ACCESSION   DASZMM010000242 DASZMM010000000
VERSION     DASZMM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5032173"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VG754_05735"
     CDS             <1..644
                     /locus_tag="VG754_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HWF18746.1"
                     /translation="ASRLYLAMRHFFETENLDAMTIRCWPEMPNTFGQWPYLGVARLC
                     EEKRALAVEGDADGALTAWIGEKLGMGRSYLTDWLEHDKETITVWHGGAAPMSLCQPQ
                     GAPGAPHIARHFNIKKPAVVEATLRENMPITIARLWRCDGKYLLTAREGSTIRPKRHL
                     MATNGLARMNNQDPHVWFEELCHAGMPHHVALFEGHHETVLRAFARALKIEFI"
     gene            652..2160
                     /locus_tag="VG754_05740"
     CDS             652..2160
                     /locus_tag="VG754_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWF18747.1"
                     /translation="MGIRAAIAIGLVLYVVLTTGVSFFWMARVKKPADYLVAGRGLPV
                     WALVGTIIGTCIGTGVIIGGAGLAYQHGWAGCAYPIGLGLGTLLAGLFFAGMRRWKFM
                     TLSEEIACYYEGNRAVVEFSNVTLFLSQLCWLTVQIKGGAAVLGVVTGLSPEICTVLA
                     GFAKAAISIPGGLKAVVYTDVLQTVILFCGFGFLMYSALIGAGGLSGLQHSVPPDYFS
                     FLGIASYGAWSVIGLILVLTLNPIADPGRRLTIYSAHSESGAKWSMVASGITVMIFSV
                     VIGITGMYTFHLNPSLKAADQALPWLIMNQLPTWLAAVVVVSVVAGISSAANGNAAAA
                     GTFYVRHIFPMVTGRFPKQPVVTVRWALAFAFFASTGLAIYTGNIVEFVKTFLPLTMS
                     GLGVVVLLGRFWKRANWQGALAALVTTPIVALAVKFTPSQLKFWHDPTIPATLVGLVA
                     QIVVSLLTAPQKRSFAEVAQAMKQERQAIEGASPDESTDTPFSQHTKPATLN"
     gene            2167..3030
                     /locus_tag="VG754_05745"
     CDS             2167..3030
                     /locus_tag="VG754_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWF18748.1"
                     /translation="MKLLNAKQICDQAADQGFGVPALNNNGGSYDIGRAIIESCQELN
                     SPLIIQCYEPNLEYCGYDYAGMLIKFLARDTTIPMAIALDHGKTPESIFRAVKAGFTH
                     VMIDYAHESIAKNVRGTQQIIDLVRPLGISVEAEIGNIIKSSDSSKQRAATVSLTEVK
                     EFTSKVDVDLLAVAVGTTHGIFKEQHGIDFRLISKIAAISPAPLVLHGTCGVSMADIS
                     KAVKAGMTKINFGEGLRMNFIRYYNEFSQTLNHEHHNWRIARAAMERLKEDIKEIICA
                     VGSEGKADKIK"
     gene            3067..4290
                     /locus_tag="VG754_05750"
     CDS             3067..4290
                     /locus_tag="VG754_05750"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWF18749.1"
                     /translation="MTNFVTHLECANCGTKYDASLVHNLCTACNRPLWVKYDLEALKK
                     AFPKSKLAGRPSSLWRYLEMLPIKDPSKIVSLNETVSPILETKRLAAEFGMSNLYVKD
                     ESRLPTGSFKSRGMTMAISKANEFGIKTVAVPTAGNAGGAMAAYAARAGMEAYVFMPE
                     DTPAINKKECYLAGAKTFLVNGLITDCGRIVGEGKKIKNWFDVSTLKEPYRIEGKKTM
                     GLEVAEQFDWELPDAILYPTGGGTGLIGMWKAFAELEALGWLASSKKPKMFSCQSEGC
                     APIAVAYAKGERFAQKFENAATIASGIRVPAAVGDFMIIDAVRQSGGVALATPEADIP
                     KWMQLAASKEGIALCPETAVCLGALEVLLKRGTLKTTDKILIFNTGASQKYPEAVNEQ
                     LPSIDITKPLDWSRI"
     gene            4303..5274
                     /locus_tag="VG754_05755"
     CDS             4303..5274
                     /locus_tag="VG754_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HWF18750.1"
                     /translation="MDILISEDLQSPALERLAKQFSVQRDGKLWEDPARLQAEIQNAR
                     VLIVRNQTQVTAKIMEAAPKLIGIGRVGVGLDNIDVNTATKLGIVVVAPLNANAVSVA
                     ELAFGLILALARKVPEGDRSTKAGGWDRRGFTGIELDGKTLGICGFGRIGGMVAARAK
                     AFGMKLMVYDPVLKADSPAVVASGATYCAQLEELLGNSDFVSLHLPLVPATKKLFNAK
                     MFAAMKPGSFFINTSRGGVMDETDLIAALLNKHLAGAALDVREMEPPGTKGVLETLPN
                     VVLMPHVGAFTIEAQKRTFEAVADDMEHLLKGQPAANFVNIAQPNRK"
     gene            complement(5286..>5556)
                     /locus_tag="VG754_05760"
     CDS             complement(5286..>5556)
                     /locus_tag="VG754_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylosidase/arabinosidase"
                     /protein_id="HWF18751.1"
                     /translation="QNQIPRLKGKFLWTQYAQAKKAGATMVYQAMFDEVDEGTAIFKC
                     TDDPPVGASTFVNYEGLPSDFYLKLVGGASRMIRGQAPLSNELPK"
BASE COUNT         1244 a         1486 c         1593 g         1233 t
ORIGIN      
        1 tggcatcgcg cttgtatctg gcgatgcggc attttttcga gacggaaaat ctcgacgcga
       61 tgaccattcg ttgctggccg gaaatgccga atacattcgg acaatggcct tatcttggcg
      121 tcgcgcggtt gtgcgaggaa aagcgcgccc tggcagttga aggcgacgcc gatggcgcat
      181 tgaccgcgtg gattggtgaa aagctcggca tgggacgcag ctatctgact gactggttgg
      241 aacacgacaa ggaaaccatc accgtctggc atggaggcgc tgcgccgatg tcgctttgcc
      301 aaccgcaagg cgcaccgggc gcgccccaca tcgctcgaca ttttaacatc aaaaaacctg
      361 cggtcgtcga agcgaccctg cgcgaaaaca tgccaatcac catcgcgcgt ttgtggcgtt
      421 gcgacggaaa atatcttttg accgctcgcg aaggttcgac catcaggccc aaacgccatc
      481 tcatggcgac taacggtctg gctcgcatga ataatcagga cccgcacgta tggttcgaag
      541 aactttgcca tgccgggatg ccgcatcacg tggcgttgtt cgaagggcat cacgagactg
      601 tgctgcgcgc ctttgcccgc gcattgaaaa tcgaattcat ttgacgccga gatgggaatc
      661 cgcgccgcca tcgcaatcgg actcgttctc tacgtcgtgt tgacgacggg agtttcgttt
      721 ttctggatgg cgcgtgtgaa aaaaccggcg gattacctcg ttgccggacg cggcttgccg
      781 gtttgggcac ttgttgggac catcatcggc acctgcatcg gaacgggcgt catcatcggc
      841 ggcgcgggcc tggcatatca acacggctgg gccggttgcg cgtatccgat tggtttggga
      901 ctgggcacgt tgctggccgg tttgtttttt gccgggatgc gccgatggaa attcatgacc
      961 ttgagcgagg agatcgcttg ttactacgaa ggcaatcgcg cggtggtcga attttcaaac
     1021 gtcaccttgt ttctgtcgca attgtgctgg ctgacggtgc agatcaaagg cggcgcggca
     1081 gtgctgggtg tggtgaccgg attatcgccg gaaatctgca cagtgctggc gggctttgca
     1141 aaggcggcga tttccattcc cggcggtttg aaggcagtcg tgtacacgga tgtgctgcaa
     1201 acggtgattc ttttttgcgg gttcggtttc ctgatgtatt cggcattgat cggcgccgga
     1261 ggattgtccg gtttgcaaca ttcagttccg ccggattatt tttcgtttct cggaatcgcg
     1321 tcttacggcg cgtggagcgt gatcggtttg attttggttt tgacgttgaa tcccatagct
     1381 gatcccggac gacggctcac catctacagc gcgcattccg agagcggcgc aaaatggagc
     1441 atggtggcct cgggaatcac ggtgatgatt ttttcagtcg tgatcggcat caccggcatg
     1501 tacacatttc atctgaatcc gagcctgaaa gctgcggacc aggcgctgcc ttggcttatc
     1561 atgaaccagt tgccgacgtg gctcgcggcg gtggtggttg tctccgttgt tgccggaatc
     1621 tcttcggcag ccaatggaaa cgctgccgca gccggaacat tttatgtgcg gcacattttt
     1681 ccgatggtca cgggacgttt tccgaaacaa ccggttgtca cggtgcgttg ggccttggcg
     1741 tttgcgtttt ttgcctcaac cggcttggcg atttacaccg gcaatattgt cgagttcgta
     1801 aagacatttt tgcctttgac catgagcggc cttggggtgg tagttttgct gggacgtttt
     1861 tggaaacgcg caaactggca gggcgctctg gccgcgctcg tgacgactcc catcgtggcg
     1921 ctggccgtga agttcacgcc ttcacaattg aaattttggc acgatccaac cattcccgcg
     1981 acgctcgttg gtttggttgc gcaaatcgtg gtcagtctat tgaccgcgcc acaaaaacgc
     2041 agctttgcag aggtcgccca ggcgatgaag caggaacggc aggccatcga aggcgcatcg
     2101 ccggatgaat caaccgacac ccctttcagt caacacacaa aacccgcaac cttgaattaa
     2161 gcaaagatga aactgttaaa cgcaaaacaa atctgcgacc aggcagccga ccagggcttc
     2221 ggcgtcccgg cgctcaacaa caacggtggc agttacgaca ttggacgcgc catcatcgag
     2281 tcctgccagg aattgaattc gccgctcatc atccaatgtt acgagccgaa cctggaatat
     2341 tgcggctacg attacgccgg gatgctcatc aaatttctcg cgcgcgacac gaccattccc
     2401 atggccatcg cgctggacca tggcaaaact cccgaatcca tttttcgcgc ggtcaaggcg
     2461 ggcttcacgc acgtcatgat tgattacgcg cacgagagca tcgcaaagaa tgttcgcggc
     2521 acgcagcaga tcattgatct cgttcgtccg cttggcatca gcgtggaagc ggaaatcggc
     2581 aacatcatca agagttccga ttcatccaaa caacgcgccg ccacagtcag ccttacggaa
     2641 gtgaaggaat tcacgtcgaa ggtcgatgtg gatttgctcg cagtcgcggt cggcacgacg
     2701 cacgggattt tcaaggagca gcacggcatt gatttccggt tgatttcgaa aatcgccgcc
     2761 atcagtcccg cgccattggt gttgcacgga acgtgcggcg tttcgatggc ggacatttca
     2821 aaggccgtga aagccggcat gaccaagatc aatttcggcg agggcctgcg catgaatttt
     2881 atccgttact acaacgaatt cagccagacg ctcaatcacg agcatcacaa ctggcgcatc
     2941 gcccgagcgg caatggagcg cctcaaggaa gacatcaagg aaatcatctg cgccgtcggt
     3001 tccgaaggca aggccgacaa aatcaaataa tttgccgaaa cagttttacc cttaatcacc
     3061 attaatatga caaacttcgt aactcatctc gaatgcgcca attgcggcac gaaatacgac
     3121 gcctcgctgg tgcataatct ttgcaccgcc tgcaaccgcc cgctttgggt caaatacgat
     3181 ctcgaagcgc tgaagaaagc atttcccaaa tccaagctcg ccggacgccc gtcgtccctc
     3241 tggcgttatc tcgaaatgct gcccatcaag gacccgtcga aaatcgtttc gcttaacgaa
     3301 acggtttcgc cgattttgga aacgaaacgg cttgcggcgg aattcgggat gtccaatctg
     3361 tatgtgaagg acgagagccg cctgccgacc ggcagcttca aatcgcgcgg catgacgatg
     3421 gcgatttcga aggccaacga attcgggatc aagacggtcg ccgttcccac cgcaggcaac
     3481 gcgggcggcg cgatggcagc ctatgcggcg cgcgcgggaa tggaagctta cgttttcatg
     3541 cccgaggaca cgcccgccat caacaaaaag gaatgctatc ttgccggcgc aaaaactttt
     3601 ctggtgaacg gcctcatcac cgattgcggg cgcatcgtcg gtgaaggcaa gaaaatcaag
     3661 aactggttcg atgtttccac gttgaaggag ccgtatcgca tcgaaggcaa aaaaaccatg
     3721 ggcctcgaag tcgcggaaca attcgactgg gaattgcccg atgccatcct ctatccgacc
     3781 ggcggcggca caggattgat cggcatgtgg aaggcattcg ccgaactgga agcactcggc
     3841 tggctggcgt cgtcgaagaa accgaaaatg ttttcgtgcc agagcgaagg ttgcgcgccg
     3901 attgctgtcg cttatgcgaa aggcgagcgg ttcgcgcaaa aatttgaaaa tgcggcgacg
     3961 attgccagcg gcatccgcgt gccggctgcg gttggcgatt ttatgatcat cgatgctgtt
     4021 cgccaaagcg gcggcgtggc gcttgcgacg ccggaagcgg acattccaaa atggatgcaa
     4081 ttggccgcct cgaaagaagg catcgcgctc tgtccggaaa ctgcggtttg tctcggcgca
     4141 ttggaagtgt tgctcaagcg cggcacgctc aagacgactg acaaaatcct catcttcaac
     4201 accggcgctt cgcagaaata tcccgaggcg gtcaatgagc aattgccgag cattgatatt
     4261 acaaagccgc tcgactggtc gcggatttga ttgaaaggcc gcatggacat tttgatttcg
     4321 gaagatttgc aaagtcccgc gctggaacgg ctcgcaaaac aattttccgt ccaacgcgac
     4381 ggcaaattgt gggaagaccc ggcacggctg caagcagaga ttcaaaacgc gcgtgtgctg
     4441 atcgtgcgca accagacgca ggtgaccgca aaaataatgg aagccgcgcc gaagttgatt
     4501 ggcatcggcc gcgtgggcgt cgggctggac aacatagatg tcaacaccgc aacgaaactt
     4561 ggcatcgtcg ttgtcgcgcc gctgaacgcc aatgcggtca gtgtggcgga actcgcattc
     4621 ggtttgatcc ttgccctggc gcgcaaggtt cccgagggcg accgttccac gaaagcagga
     4681 ggatgggacc gtcgcggatt caccggtatc gaacttgatg gcaaaacact cggcatttgc
     4741 ggctttggcc ggattggcgg aatggtggct gcgcgtgcaa aagctttcgg aatgaaattg
     4801 atggtttacg atccagtttt gaaagccgat tctccagccg ttgtcgcgag cggggcaacc
     4861 tactgcgcgc aattggagga attgcttggc aattcggatt ttgtcagcct gcatctgccg
     4921 ctcgtgcccg cgacaaaaaa attgttcaac gcaaaaatgt ttgccgcgat gaaacccggc
     4981 agctttttca tcaacacttc gcgcggcggc gtgatggatg aaacggactt gatcgcggcg
     5041 ttgctgaaca aacatcttgc tggcgcggcc ttggacgtgc gcgaaatgga gccgccaggc
     5101 acaaaaggcg ttcttgaaac attaccgaat gtggttttga tgccgcacgt tggcgcgttc
     5161 acaatcgagg cgcaaaaacg tacttttgaa gcagtggctg atgacatgga acatttgttg
     5221 aaaggccagc ctgccgcaaa ttttgtgaac atcgcgcagc cgaacaggaa atagtcgggt
     5281 tgccattatt tgggaagctc gttgctcaag ggtgcttgcc cgcgaatcat tcgcgatgcc
     5341 cctccgacga gtttcagata aaaatcactc ggcaatcctt cgtaattcac aaacgttgac
     5401 gcgccaaccg gcggatcatc ggtgcatttg aagatcgcgg ttccctcgtc cacttcatcg
     5461 aacatcgcct ggtaaaccat cgtcgctccg gcttttttcg cctgcgcata ttgcgtccag
     5521 aggaatttgc ccttgagccg ggggatctgg ttttgc
//
LOCUS       DASZMM010000243         6516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5310791, whole genome shotgun sequence.
ACCESSION   DASZMM010000243 DASZMM010000000
VERSION     DASZMM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5310791"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VG754_05765"
     CDS             complement(<1..259)
                     /locus_tag="VG754_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF18752.1"
                     /translation="MQTEPAQQNASPEKPVAAGVSDLLLTTDKLVKEYRGRRVVNGVS
                     IHVGAGEIVGLLGPNGAGKTTTFNMVVGVVKPGEGAVKFLDR"
     gene            complement(259..2430)
                     /locus_tag="VG754_05770"
     CDS             complement(259..2430)
                     /locus_tag="VG754_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HWF18753.1"
                     /translation="MPQKIHQSLFVQWWGLVALCFFVFVISSSAQPVLQHEKHGRQLV
                     PYDPPNDQQVKIELTYAAADLATNGSGNLQWKIAQIKQFPVTGGDPQMVAETPECVSD
                     QRLDEITSTNSLTVHANRGSIFLEGVGFLFHHTNMSLVISNKVHSVLRLRPATNAPAP
                     ATDIFSDRGEFEMKTDATNGMALYIRNVHVLDPQMKLDCDWLRADLPKDQAGQTNRPN
                     HIVARTNVVIDFANTNGEKIHAVGQQAVYDYRVANAATNESLELTGNPRVDLTNGWMT
                     ADTLVMDRATGKLRGVGNFHFHYHPPSQAVETEIVSDLFDYDSTTRLANFWGNVRVKD
                     PRLKLTSEFLTATLPAQQSGGTNHIDHILAETNVVMDFAVGTSTNKQETHASGQKAVY
                     DYKVKGGVTNEVMELTGNPGVQLDKGWMTADFIKVDRAQGRVWGTGSHHSVIKKQPGE
                     TAMMDTEIFSDNFEYATTNGIALYSGNVRAYDPELNLTQAKLLTVELAGGAKGATNIH
                     KIIAGGNVALDFAAKAFATGDITNLPAFAARLKSPQDAPAKFVSAQLTPPTQKLLAVY
                     TGGTNAQLQKALAEDLNRITQNGPVYEPARFTNVFLSVETSRLMKENAMGLDLIQLNR
                     LLLLDAFRGVLARGRLGEKTHATGERAVYTSQGPNASTSALLELYGHPKLFNPSGWME
                     ADDSIIYNRLTGVGQYLGNPKFHPNIVGLVKRSGTAKNGTQ"
     gene            complement(2433..2987)
                     /locus_tag="VG754_05775"
     CDS             complement(2433..2987)
                     /locus_tag="VG754_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWF18754.1"
                     /translation="MRRLIIMPNDISIELNARLAKVKLFLCDVDGILTDASVFIGLPE
                     EIKRFHIRDGLGLIMLRAQGIKVGWVSKRPSSATTRRAEELKIDFLEQGKGSKVSAVE
                     NLLAQTGFQWDEVCYMGDDIVDLGVLKRAGVAATVADATYEARAASHYVTLANGGCGA
                     VREVVELILKAQNRWQKLVAEESA"
     gene            complement(3285..4103)
                     /gene="kdsA"
                     /locus_tag="VG754_05780"
     CDS             complement(3285..4103)
                     /gene="kdsA"
                     /locus_tag="VG754_05780"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HWF18755.1"
                     /translation="MINDSAIWKSLSSSRHLILIGGPCAIESEALCLKVASSLKKTCE
                     KLGITYIFKASYDKANRTSNQSFRGPGLEAGLEILAKVRASLGIPVLTDVHSELQVAA
                     AGAIADALQIPAFLCRQTDLIVAAIKTGRIVNIKKGQFLSPLEMGQVVKKAESAGGKK
                     IMVTERGTTFGYNNLVSDMRSIPIMRRFGYPVIFDATHSVQLPGGGGDKSSGQREFAP
                     VLARSAVAAGASGVFIETHPDPDHALSDGPNMIPLAQMPKLLASLVKVREAIQA"
     gene            complement(4245..4439)
                     /locus_tag="VG754_05785"
     CDS             complement(4245..4439)
                     /locus_tag="VG754_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18756.1"
                     /translation="MSENEKREPAQKSETERKPSANPSRDSKPSAPKPESKPQRKPGP
                     SVDIGPGPGADVVATPPRRI"
     gene            4458..5366
                     /locus_tag="VG754_05790"
     CDS             4458..5366
                     /locus_tag="VG754_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWF18757.1"
                     /translation="MQNAQAGLCCFIEEKGWGMHPVYIGTSGFSYKSWGNTFYPEDVP
                     KYRQLEFYQTQFPTVEINATFYRLATLAMTRRWHDVAPEGFLYAVKGSRFITHIKRLI
                     AVKEALDKFFDRIAPLKEKLGPILWQLPPTMKKDCARLEDFLRLLPKTRRHAVEFRHL
                     SWFEDDEALEILKKHCAAFVSVSSGKMPVKLAVTADFIYIRFHGLAGGAAHDYTRAEL
                     KPWADHIKAHPDKTVFTYFNNDVNVRAPQNARELMSMVGERAFGPFASEMPDLPRKHG
                     RAVKKNKEERTHKLRKKIRKGVLVSG"
     gene            complement(5443..5895)
                     /locus_tag="VG754_05795"
     CDS             complement(5443..5895)
                     /locus_tag="VG754_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HWF18758.1"
                     /translation="MKQTKETADLLERAIEIAVQAHHGQRDKNGAPYILHPLRIMNAV
                     RRPEEKMVAILHDVVEDSDWTLARLRDEGFPVDVLEAVDHLTRRDGESDEAFVNRAVS
                     HPLAKKIKICDLEDNLNPLRNSQLDEKAIAKLVRHHQSWHRVTAREFY"
     gene            6094..>6516
                     /locus_tag="VG754_05800"
     CDS             6094..>6516
                     /locus_tag="VG754_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWF18759.1"
                     /translation="MSKTVHFLLAVAALVLTSCSSMKKPVAAANPKPDIENLSARKYT
                     ATVRADYLLFLPKDYDAHSAKRWPTIMFLHGSGERGSNVWRATIHGPTKFIEKHPDFP
                     FILITPQCPEGRKWSDEIVLGILDAVTAKYAVDTNRIYL"
BASE COUNT         1401 a         1757 c         1815 g         1543 t
ORIGIN      
        1 cgcggtcgag aaatttgacc gcgccttcgc cgggtttgac gacgcccacg accatgttga
       61 atgtggtggt cttgcccgcg ccgttgggac cgagcaaccc gacaatctca cccgcgccga
      121 catgaatgga gacgccgttg actacccgac ggccgcgata ttccttcacc agcttgtccg
      181 tggtgagcag caggtcggag acaccggcgg cgacaggttt ttccggggag gcgttttgtt
      241 gtgccggttc agtttgcatc actgagttcc attctttgcc gtgcccgacc ttttgacaag
      301 acccacaata ttcggatgaa atttcgggtt gccgagatac tggccgacgc cggtgagccg
      361 gttgtagatg atggaatcgt cggcttccat ccaaccgctg ggattgaata attttggatg
      421 tccgtaaagt tcaaggagcg cgctggtgga agcgttggga ccctggctgg tgtaaacggc
      481 gcgttcgccg gtggcgtggg tcttctcgcc gagacgcccg cgcgccaaca cgcctcgaaa
      541 agcgtccagc aacaacagcc ggttcaactg gatcaaatca agccccatcg cattttcttt
      601 catcaaccgg gacgtttcga cggaaagaaa gacatttgtg aaacgcgcgg gttcataaac
      661 cgggccgttt tgagtgatgc ggttgaggtc ttccgcgagc gctttttgga gttgcgcatt
      721 tgttccgccg gtgtaaacgg caagcagttt ttgcgtgggc ggagtgagtt gcgcgctgac
      781 aaatttcgcg ggggcatctt gcggcgattt caggcgcgct gcaaatgcgg gcaaattggt
      841 gatgtcgcca gtcgcgaaag ccttcgctgc gaagtcgaga gcgacatttc ccccggcaat
      901 gatcttgtga atgttggtcg cgcctttcgc gcccccggcc aattccaccg tgagcagttt
      961 cgcctgggtg agattcagtt caggatcata cgcgcgcacg tttccgctat aaagcgcgat
     1021 gccgttcgtc gtcgcgtatt cgaaattgtc ggaaaaaatt tccgtgtcca tcatcgcggt
     1081 ttcgccaggc tgttttttga tgaccgaatg atgactgccc gtgccccaaa cgcgtccttg
     1141 cgcgcggtcc accttgatga agtccgcggt catccagccc ttgtccaatt gcacgcccgg
     1201 attgccggtc aactccatca cctcattggt gacgccgccc ttgaccttgt aatcataaac
     1261 ggccttttgc ccgctggcgt gtgtctcctg cttgttcgtg gaagtgccga cggcaaaatc
     1321 catgacgaca ttcgtttcgg ccaaaatgtg gtcaatgtga ttggtgccgc ccgactgttg
     1381 cgccggaagc gtggcggtca agaattcgga ggtgagtttc aatcgcgggt cttttacgcg
     1441 aacatttccc caaaaatttg ccagccgcgt cgtgctgtca tagtcaaaca ggtcggagac
     1501 aatctcggtt tccacggctt ggctcggcgg atgataatgg aaatgaaaat tgccgacgcc
     1561 gcgcagtttg ccggtggcgc ggtccatgac caatgtgtcg gcggtcatcc aaccattggt
     1621 caaatccacg cgcgggttgc cggtgagttc cagcgattca ttggttgcgg cattggccac
     1681 gcggtaatca taaacggcct gctggccgac ggcgtggatt ttttcgccgt tggtgttcgc
     1741 gaaatcaatg acgacattcg tgcgcgcgac gatgtggttg gggcgattgg tctggccggc
     1801 ctggtccttg ggaagatcgg ccctgagcca gtcgcaatcc agtttcattt gaggatcgag
     1861 gacgtggacg ttgcggatgt aaagcgccat gccgttggtc gcgtcggtct tcatctcgaa
     1921 ttcgccgcga tccgaaaaaa tgtcagtcgc tggcgctggc gcatttgtgg cgggccggag
     1981 tcgaaggacg gaatggacct tgttggaaat gacgaggctc atgttcgtgt gatgaaaaag
     2041 gaaaccaacg ccttccagaa aaatgctgcc gcgattggca tgcaccgtca gtgaatttgt
     2101 cgaggtgatt tcgtcaaggc gctggtctga cacgcattcg ggcgtctcag ccaccatttg
     2161 cgggtcgccg ccggtgacgg gaaattgttt gatctgggcg attttccatt ggagattgcc
     2221 gctgccgttt gttgcgaggt ccgccgccgc ataggtaagt tcaattttca cctgctggtc
     2281 gttcggcggg tcgtaaggga ccaattgacg gccgtgcttc tcgtgctgaa ggaccggttg
     2341 cgcggaagag gagatgacaa acacaaaaaa gcagagagca accaatcccc accattggac
     2401 aaacaaactt tgatgaattt tttgcggcat gatcaggcgg attcttccgc aaccaatttt
     2461 tgccagcggt tttgcgcctt caaaatcaat tccaccactt cgcgcacagc gccgcaaccg
     2521 ccgttggcaa gcgtcacata atgagaggcg gcccttgcct cgtacgtggc atcggcgacc
     2581 gtggccgcaa cgcccgcccg cttcaacacg ccaagatcca cgatgtcatc ccccatgtaa
     2641 cagacttcgt cccactgaaa acctgtttgg gcgagcaggt tttcgacggc ggacactttg
     2701 ctgcccttgc cttgctccaa aaaatcgatt ttcagttctt ccgcgcggcg cgtcgtcgcg
     2761 gacgagggcc gcttcgaaac ccagccgacc ttgatgccct gtgcacgcaa catgatcagc
     2821 cccaaaccgt cgcggatgtg gaaccgcttg atctcctccg gcaacccgat gaaaacagac
     2881 gcatcggtga gaatgccgtc cacatcgcaa agaaataatt tcaccttcgc cagccgcgca
     2941 ttgagttcga ttgaaatgtc gttgggcatg atgatcagcc tgcgcaaagc aaacgtgttg
     3001 gcaatttggt tgaaggttcg ccctcacccc ggccctctcc cccaggagag ggggaacgca
     3061 atggccgttg cggagttatc gagcgattcg gttgcaattt ccacgtatta aacgaccgca
     3121 aagagaagct gcagccagtt ccgccattca acaccgggca gcgcgagacg gtactccctc
     3181 tcccggaaga gagggccggg ttgagggcga gtattcgcac tgactttatc atttgtcctg
     3241 ataacttgtt ttatggagag ttccgaacat tcaatttttc cttctcatgc ttggatcgct
     3301 tcgcgcactt ttacgaggct ggcgagcagc tttggcattt gggccaatgg aatcatgttc
     3361 gggccgtcgc tcagggcgtg atcgggatcg ggatgtgtct cgatgaaaac gccgctcgcg
     3421 cctgcggcga ccgcgctgcg cgccagcacc ggcgcaaatt cccgttgacc gctcgatttg
     3481 tcgccgccgc cgccgggcaa ttgcaccgaa tgcgtcgcgt caaaaatcac cggataacca
     3541 aaacgccgca tgatgggaat ggaacgcatg tcggacacca gattgttgta gccgaacgtc
     3601 gtgccgcgtt cggtgaccat gatttttttg ccgccggcgg attcggcttt tttgacgact
     3661 tgccccattt caagcggcga caaaaactgg cctttcttga tgttcacgat acggcctgtc
     3721 ttgatggcgg cgacgatcaa atcggtttgg cggcaaagaa aggcgggaat ttgcaacgcg
     3781 tcggcgatgg cgccggcagc ggcgacctgc agttcggaat gcacatccgt gagcaccggg
     3841 atgccaagcg aggcgcgcac tttcgcaaga atttccaagc ccgcttcgag gccgggacca
     3901 cgaaaagatt ggttggaggt gcggttggct ttgtcataac tggccttaaa aatgtaggtg
     3961 atgcccagct tctcgcaggt ttttttcagc gaggaagcga ctttaaggca gagcgcttca
     4021 ctttcaatcg cgcacgggcc gccgatcaaa atcaaatgcc gggatgaact cagggatttc
     4081 caaatcgcgg aatcgtttat cacgccatgt tcttaacatc gcctgcacgg gatgtaaaag
     4141 ccccaaattc ccgccaaaag acaaataatt gggaaatcag gaactcagga aggcagattt
     4201 ttttcaatct tgatttcctg gtttcctaat tctatccggt aaaactaaat tcttctcggc
     4261 ggcgttgcca cgacgtccgc gccggggccg gggccgatgt caacgctggg gccgggtttg
     4321 cgctgtggct tggattcagg tttgggcgca gacggttttg agtcgcgaga cggattggcc
     4381 gaaggcttgc gttcggtttc agatttctgc gccggttcgc gcttttcatt ttcgctcatg
     4441 caaaaaaata atttaaaatg caaaacgcac aagccgggtt atgttgtttt attgaagaaa
     4501 aaggatgggg tatgcacccg gtttatatcg gcacgagcgg attttcttat aaaagttggg
     4561 gaaacacgtt ttacccggaa gacgtgccga aatatcgcca actggaattt taccagaccc
     4621 agtttcccac cgtcgaaatc aacgccacat tttatcgtct cgcgaccctt gcgatgacgc
     4681 gccgctggca tgatgtcgcg cccgaaggct tcctgtatgc cgtgaaaggc agccggttca
     4741 tcacgcatat aaaaagattg attgcagtca aagaagcgct ggacaagttt ttcgaccgga
     4801 ttgcgccact gaaggaaaag ctcgggccaa tcctttggca attgccgccc acgatgaaaa
     4861 aagattgcgc gcgattggaa gattttttgc ggctgctgcc caaaacgcgg cgtcacgccg
     4921 tcgagttccg ccatctttca tggttcgagg atgatgaagc ccttgaaatt ctgaaaaaac
     4981 actgcgctgc atttgtatcg gtgagttccg gcaaaatgcc ggtgaagctc gcggtgacgg
     5041 cggatttcat ttacatccgt tttcatggat tggcaggcgg cgccgcgcat gattacacgc
     5101 gcgccgagtt gaagccatgg gccgaccaca tcaaagccca tcctgacaag actgtgttca
     5161 cgtatttcaa caacgatgtg aatgtccgcg cgccccagaa tgcccgtgaa ctcatgagca
     5221 tggttggcga acgcgcgttc gggccgttcg ccagcgagat gcctgattta ccgcggaaac
     5281 acgggcgcgc ggtgaagaag aataaggaag aacgaactca caaactcagg aaaaaaatcc
     5341 gaaaaggtgt gctggtcagc ggatgaggca ggaattttct tgcgcccacc cttcattgag
     5401 ttcctgagtt catgagttcc ttcttaaatc ttagtttgtc acttaataaa actctcgcgc
     5461 ggtcacgcga tgccatgatt gatgatgccg gacgagcttg gcgatggctt tttcgtcaag
     5521 ctgactgttg cgcaacggat tcagattgtc ttccaggtcg caaatcttga ttttcttcgc
     5581 gagtggatgg ctgacggcgc ggttcacgaa tgcttcatcg ctttcgccgt cgcgacgcgt
     5641 caggtgatca acggcttcga gcacatcaac gggaaaacct tcatcgcgca atcgcgccag
     5701 cgtccaatca ctgtcctcca cgacatcgtg caaaattgcc accatctttt cttcgggccg
     5761 acgcaccgcg ttcatgattc gcaacggatg aagaatgtat ggcgcgccat ttttgtcccg
     5821 ctgaccgtga tgcgcctgca ctgcgatttc aatcgcccgt tccagcaagt ccgccgtctc
     5881 ctttgtttgt ttcattgagc aaaataaacc attcgccgct ccgcttccaa aaaaggtttc
     5941 aaattataat gggctgttca ccgacggatg cttaatttgc agcagccggg gaggtgggtc
     6001 ggtttccgcg aagttcaaag tttaaagtcg gacagtcctg ggtctgttgg tgctgacctt
     6061 gcgcataggc cgtcctctgt ttataaaatc gacatgagta aaaccgttca tttcctgctt
     6121 gctgtcgcgg cgttggttct tacatcgtgc tcctccatga aaaagcccgt cgctgctgcc
     6181 aatcccaagc ccgacataga aaatctttcc gcccgcaaat acacggcgac tgtccgcgcc
     6241 gattatctgc tttttctgcc caaggattac gatgcgcaca gcgccaagcg ctggcccacg
     6301 atcatgtttc tgcacggttc gggcgagcga ggcagcaatg tctggcgcgc aaccatccat
     6361 gggccgacca agtttatcga aaaacatccc gattttccgt tcatcctcat cacgccgcaa
     6421 tgtcccgaag gccgcaaatg gtcggacgaa atcgtgctcg gcattcttga cgcggtgaca
     6481 gcgaagtatg ccgtggacac gaaccggatt tatttg
//
LOCUS       DASZMM010000244         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2292854, whole genome shotgun sequence.
ACCESSION   DASZMM010000244 DASZMM010000000
VERSION     DASZMM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292854"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VG754_05805"
     CDS             complement(<1..921)
                     /locus_tag="VG754_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWF18760.1"
                     /translation="MTETAITPALIKQHGLTPEEFERIKGVLGREPNYTELGIFSVMW
                     SEHCSYKNSRPLLKTFPTKSPKILVGAGEENAGIIDIGDGLAIAFKIESHNHPSAVEP
                     FQGAATGVGGIIRDIFTMGARPICSINSLRFGPITNGASGPDAVPEAQKIGATAKVSS
                     DITSAPPIENQKSQIANNKRLFAGVVAGIAHYGNCFGIPTVAGEVYFDPSYEGNPLVN
                     AFCLGVLRHEEIARGAAKGMGNPVFYVGPATGRDGLAGAAFASQDLTDESAEQQRGAV
                     QVGDPFMEKLVMEACLELLATDAVAGIQDMG"
     gene            complement(931..1257)
                     /locus_tag="VG754_05810"
     CDS             complement(931..1257)
                     /locus_tag="VG754_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWF18761.1"
                     /translation="MKNANIFVDVDLTLVDANGKVLSGAAEALSKMKAKGCHLFLWST
                     NGAEYARKVAAVCGLSEMFEGCVAKPDIIIDDMPATVLNPFVFDVSHEESWPALAEKI
                     IRKHID"
     gene            complement(1293..1517)
                     /locus_tag="VG754_05815"
     CDS             complement(1293..1517)
                     /locus_tag="VG754_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18762.1"
                     /translation="MSTLQIQKALTAWPQVRPALFVPHHEREYRQLVKLLDRLIDEVG
                     EDENHPLASLTEVIGVLIKKYEDEKVPTLI"
     gene            complement(1520..1729)
                     /locus_tag="VG754_05820"
     CDS             complement(1520..1729)
                     /locus_tag="VG754_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF18763.1"
                     /translation="MRYTIIIEPGPGNFSAYAPDFPGCVAAGDTEQETLTLMKKALEM
                     HIEDMRQRGEQIPQPSMAREIEVAA"
     gene            complement(1733..1918)
                     /locus_tag="VG754_05825"
     CDS             complement(1733..1918)
                     /locus_tag="VG754_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002752119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWF18764.1"
                     /translation="MKIREIMRRIEDDGWFLVRTRGSHRQYHHATKPGTVTIAGHPGQ
                     DLHPKTLNSILKQAGLK"
     gene            complement(1922..2635)
                     /gene="purQ"
                     /locus_tag="VG754_05830"
     CDS             complement(1922..2635)
                     /gene="purQ"
                     /locus_tag="VG754_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWF18765.1"
                     /translation="MNFAVLQFPASNCDQDAVHVLRNVLGHSARLLWHKEISLGNADA
                     VIVPGGFSYGDYLRTGAIARFSPVMQAVQTFAANGGPVLGICNGFQILCEAGLLPGVL
                     IRNRSLQFRCEHVFIKPLTADSPFTNRIPSGKLLRVPIAHGEGCYFADEETIGQLKSK
                     NQILWQYVNAGGEATDAANPNGSLQNIAGICNERRNVAGLMPHPERASEPLLGSADGR
                     LIFESLIQSLESRDVATAA"
     gene            complement(2640..2876)
                     /gene="purS"
                     /locus_tag="VG754_05835"
     CDS             complement(2640..2876)
                     /gene="purS"
                     /locus_tag="VG754_05835"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWF18766.1"
                     /translation="MKAKIIITPKKAVLDPQGKTVQNALAHMGYEGIGAVHVGKYLEI
                     EVNGDRETVRKQLDEACHKFLSNPVIEDYKLELE"
     gene            3023..3292
                     /locus_tag="VG754_05840"
     CDS             3023..3292
                     /locus_tag="VG754_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18767.1"
                     /translation="MKIDYTVQVWQEGSQYIAHAMPLDVASSGETPELARQAVDEAVR
                     LFLATSAEHGTLKEILEDAGYHLAAKNWRGPVWLGVEQRSCLTEV"
     gene            3301..3534
                     /locus_tag="VG754_05845"
     CDS             3301..3534
                     /locus_tag="VG754_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWF18768.1"
                     /translation="MPKFTPEHWRTVEAIFLAAGFTFARQEGSHRSYVKPGVARPVVI
                     PTYKEVPVFVIRNNLKTAGLTRDDYFALRAKVR"
     gene            complement(3539..>4434)
                     /locus_tag="VG754_05850"
     CDS             complement(3539..>4434)
                     /locus_tag="VG754_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWF18769.1"
                     /translation="TVVDKTGRTLADVLTDIGLNYLRGTPNGIRMEAASGLIWSPSHF
                     TWMDTNHPAGTPREGYPVEIQVLWIRLLRQLQHIGNASERKRWGELAAQAQASLLKYF
                     WFEDGSYLADLLIAKPGQSAREATVDNALRSNCLLAVSLGLLTGEPARCCVAAAQRYL
                     IVPGALRTLAPLPVSPPLPIYGNQDQLLNDPLKPYQGHYEGDEDTRRKPAYHNGTAWT
                     WTFPIFCEALARAWDFAPEAVAAAKAYLGSMDRLLMEGCIGQLPEIVDGDVPHTQRGC
                     DAQAWGATEALRVWKLLQQGR"
BASE COUNT          959 a         1254 c         1181 g         1040 t
ORIGIN      
        1 gcccatgtcc tggattcccg ccacggcgtc cgtcgcgagc aattccaggc acgcttccat
       61 caccaatttt tccatgaacg gatcgcccac ctgcaccgcg ccgcgttgtt gctcggcgga
      121 ttcatccgtc aaatcctgcg acgcgaacgc cgcgcccgcc aatccgtcgc gacccgtcgc
      181 cgggccaaca taaaacaccg gattgcccat gcctttggcc gcgccgcgcg caatttcctc
      241 atgacgcaac acgccgagac aaaacgcgtt caccaacgga ttgccttcgt aagatggatc
      301 gaaataaacc tcgcccgcaa cggtcggaat cccgaaacaa ttcccgtaat gcgcgatgcc
      361 cgcgaccacc cctgcgaaca gacgcttgtt atttgcgatt tgcgattttt gattttcgat
      421 tggtggcgcg ctggtgatgt cgcttgaaac ttttgcggtt gcgccgattt tttgcgcttc
      481 cgggacagca tcaggaccgg acgctccgtt cgtgattggg ccgaaccgca gcgaattgat
      541 ggaacaaatc ggccttgcgc ccatggtgaa aatatcgcgg atgatgccgc ccacgcccgt
      601 cgccgcgcct tggaaaggtt ccaccgcgct gggatgattg tgcgactcaa tcttgaacgc
      661 gattgccagc ccgtcgccga tgtcaatgat gcccgcgttt tcctcgcccg cgccgacgag
      721 aattttcggc gacttggtcg ggaacgtttt gagcaatgga cggctgtttt tgtaggaaca
      781 atgctcgctc cacatcaccg agaaaatacc cagctcggtg taattcggct cgcgcccaag
      841 gacgcccttg atgcgctcga attcctctgg cgtcaagccg tgctgtttga tcaatgcggg
      901 tgtgatggcg gtttcggtca tttcaattat tcaatctatg tgcttgcgaa tgattttctc
      961 ggccaatgca ggccacgact cttcatggct tacatcgaaa acaaagggat tcaaaacggt
     1021 tgcgggcatg tcatcaatta tgatatccgg ctttgcgacg caaccttcga acatttccga
     1081 caagccgcaa acagcggcaa ctttacgggc atattcggcg ccgttcgttg accataaaaa
     1141 taaatggcaa cccttcgctt tcattttgga caacgcctcg gctgcaccag acaaaacctt
     1201 tccattggca tccaccaacg tcaggtcaac gtccacaaaa atattggcgt ttttcattcc
     1261 ggaaccgcaa agctcatgga cataaataat gttcagatta aggttggcac tttttcatcc
     1321 tcatattttt taatgagaac gccgatgact tccgtcagcg acgccagtgg atgattttca
     1381 tcttcaccta cttcatcaat gaggcggtca agtaatttaa ccagttggcg atattcccgc
     1441 tcgtggtgtg gcacgaacaa ggccggccga acttgaggcc aggcggtcag cgctttttga
     1501 atttgcaagg tggacatgtt tacgccgcca cttcaatttc ccgcgccatg cttggttgtg
     1561 gaatttgctc tccgcgctgg cgcatgtctt caatgtgcat ttccaacgct tttttcatca
     1621 aagtgagtgt ttcctgttcc gtgtcgcccg ctgccacgca accgggaaaa tcaggagcat
     1681 aagcgctgaa attgcccgga cctggttcga taataatggt atagcgcata atttatttga
     1741 gtcccgcttg cttcaggata ctgtttaatg ttttggggtg caaatcttgt ccgggatggc
     1801 ccgcaatcgt aacagtgccc ggctttgttg catgatgata ttgccggtga ctcccgcggg
     1861 tgcgaactag aaaccagcca tcatcttcaa ttcgccgcat aatctcacga atcttcatta
     1921 tttacgccgc tgtcgccacg tcccggcttt ccagcgactg gatcaaactt tcaaatatta
     1981 atcgtccgtc cgcgctgccg agcaacggtt cgctggcgcg ttcgggatgg ggcatcaggc
     2041 cggcgacgtt gcggcgttcg ttgcaaatgc cggcgatgtt ttgcagtgaa ccattcgggt
     2101 tcgccgcgtc cgtcgcttcg cctccggcat tcacatattg ccagaggatt tggttcttgg
     2161 atttgagctg gccaatggtt tcctcatcgg cgaaatagca tccttcgcca tgggcgatgg
     2221 gcacgcgcaa caatttgccg gacggaatcc ggttggtgaa cggcgaatcg gccgtgagcg
     2281 gcttgatgaa aacatgttcg caacggaatt gcagcgagcg gttgcggatg agcactccag
     2341 gcaacaaacc ggcctcgcac aagatttgga acccattgca aatccccagc accggcccgc
     2401 cgtttgcggc aaaggtttgc accgcctgca tcaccgggct gaaccgcgcg atggcgccgg
     2461 tgcgcagata atcgccgtag ctgaatccgc ccggcacgat gacggcgtct gcgttgccga
     2521 gcgaaatttc cttgtgccac aacaatcgcg cggaatggcc gagcacattg cgcaagacat
     2581 gcacggcgtc ctggtcacag ttcgaggcgg ggaattggag gacggcaaag ttcatcggtt
     2641 cattccagtt ccagtttgta atcttcaatc accgggttgc tcaaaaattt gtggcaggct
     2701 tcgtccaatt gtttgcggac agtttcgcgg tcgccgttga cctcgatctc gagatatttc
     2761 ccgacgtgga ccgcgccgat gccttcatag cccatgtggg caagggcgtt ctggacggtc
     2821 ttgccctgcg ggtcgaggac ggcttttttt ggcgtgatga tgatttttgc tttcatgtcg
     2881 ttgcggacga aaactcaaga cgaatcattc gttaatatgt tgcggaaagc aagacgcgga
     2941 cgctagaaat ttttttgcgg agcgagttcc caaatggaca taatgaagtt gagccggtgg
     3001 aggcaaaatg ttatcttgcg acatgaagat tgattacacg gtgcaggtgt ggcaggaggg
     3061 ttcccagtac attgcgcacg ccatgcctct cgacgtggca agttcgggcg agacaccgga
     3121 attggcccgg caagcagttg atgaagcggt ccgtttgttc ctcgccactt ccgccgaaca
     3181 cggtacgttg aaggaaattc tggaggatgc cgggtaccat ctcgccgcaa aaaattggcg
     3241 cgggcctgta tggctgggcg ttgagcaacg cagttgcttg actgaggtat aattcgcttc
     3301 atgcccaagt tcactcctga acactggcgc actgttgaag caatattcct tgctgctggt
     3361 tttacttttg cgagacagga aggcagccat cgctcatacg tgaaaccagg agtggcgcgc
     3421 ccagttgtta ttccaaccta caaagaagtt cccgtttttg tcattcgcaa caatctcaag
     3481 acagccggac tcacacgcga cgattacttt gctttgcgcg caaaggtacg ctgaaacttc
     3541 agcgcccctg ctgcaacaac ttccacaccc gcaacgcctc ggttgcgccc catgcctgcg
     3601 cgtcgcaacc gcgttgggta tgcggcacgt cgccatccac gatttcagga agctggccga
     3661 tgcagccttc catcaacaaa cggtccatgc tgcccaggta agcctttgcg gcagcgaccg
     3721 cttccggcgc gaaatcccac gcgcgcgcca gcgcttcgca aaagattggg aatgtccacg
     3781 tccacgccgt gccgttgtga taagccggct tgcggcgtgt gtcttcatct ccttcgtaat
     3841 gcccctgata aggtttgagt gggtcattca gaagctggtc ttgattgcca taaatgggca
     3901 atggcggcga aacgggcaac ggggccaatg tgcgcaacgc tccgggaaca atcaaatacc
     3961 gctgcgccgc cgcgacacaa catcgcgcgg gttcgcccgt caacaaacca aggctcacgg
     4021 ccagcaggca attgctccga agcgcgttgt ccacagtggc ttcgcgcgcc gattgacccg
     4081 gtttggcgat cagcaggtcc gccagataac tgccatcctc gaaccaaaaa tattttagca
     4141 gcgacgcctg cgcttgcgcg gcgagttcac cccagcgttt gcgttcggaa gcattgccga
     4201 tgtgttgcaa ctggcgcaac aagcgtatcc acagcacctg aatctcgacc ggataacctt
     4261 ctcgcggcgt gccggcagga tgattcgtat ccatccaggt aaaatgactg ggactccaaa
     4321 tcaaaccgga agcggcctcc atgcgaatgc cattgggcgt gccgcgcaga taattaaggc
     4381 cgatgtcggt caaaacgtca gccagcgtgc gaccagtttt atctactaca gtcc
//
LOCUS       DASZMM010000245        10293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_379581, whole genome shotgun sequence.
ACCESSION   DASZMM010000245 DASZMM010000000
VERSION     DASZMM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379581"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VG754_05855"
     CDS             <1..421
                     /locus_tag="VG754_05855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18770.1"
                     /translation="LSCENVFLAKFPPGSFDFIYSLGMFGHGCPTTSELCNRFHDWLA
                     PGGQLFFNVVSLHSMSFVQRVRRSIRTKLYPLLPRQIKKALDAREARVPFFGLSGKDL
                     AGIMRASRFGDYSISSHVYHSQLWRNVHLECQAIKSR"
     gene            complement(445..1284)
                     /locus_tag="VG754_05860"
     CDS             complement(445..1284)
                     /locus_tag="VG754_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18771.1"
                     /translation="MNITGNAEDVFSRQNKPFRIPRAAAFTLIELLVVVAIIAILASL
                     LLPALARAKSRAWEAACLNNLNQLQTCWHLYTMDNSEYLVPNNSVEGITTNGGEGSIA
                     TGVSWCLADPTEPNVENGMLFPYNTSLGIYHCPADHSTLTDPSGRDNGTLRSRSYNMS
                     QSVNGYNWFLSGYIPCFVKTTDIRNPNVANCLVFIDENEYTLLDSQFGMPTDFYDRST
                     YWWDMPANRHDQGANLSFADGHVERFKWVVPKTFTSWITSVSPQEMPDWLRVKACIKQ
                     DMN"
     gene            1820..2245
                     /locus_tag="VG754_05865"
     CDS             1820..2245
                     /locus_tag="VG754_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWF18772.1"
                     /translation="MNKKSAKITALIEGCRGQDLDAHYLGFFECFNQTLFYEAHDVLE
                     ELWLANRHSSNYSFHKGLIQLAGAFVHLQKNRLKPAAALFKLARTNLQKYPERHEHLD
                     VSETLLLIERWLQELESNDFITNPLKANNAPKLHLLKRD"
     gene            complement(2242..2724)
                     /locus_tag="VG754_05870"
     CDS             complement(2242..2724)
                     /locus_tag="VG754_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18773.1"
                     /translation="MQPEGLPEISRGFGATIPPGNEIKQTTLKTSNKLHNVCLDKPFQ
                     PQIPSCKKGWPANRMNDFRTFRKSSVRYWERRRIFLQFDAHPPAFAGYSIATTVNFVG
                     NPAQPYFAYVLAQLAISALRANICFSFPYSLEAFSRGLKNGFVSRNFMRMKFSLAVES
                     "
     gene            2942..6742
                     /gene="smc"
                     /locus_tag="VG754_05875"
     CDS             2942..6742
                     /gene="smc"
                     /locus_tag="VG754_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWF18774.1"
                     /translation="MYLKNLTVLGFKSFANKTSLNFQPGVTAIVGPNGCGKSNVSDAV
                     RWVLGEQSAKALRGGEMADVIFNGTDGRKPLGMAEVSLTIGGVDDEHLMAAGVEVAYN
                     EVTITRRVFRDGGSEYFINKTPCRLKDVQQLFMGTGVGRTSYSIMAQGNITQILSSKP
                     EDRRMIFEEAAGITKFKAQKKESLRKLEYTEQNLLRVADLIREVKRQIGSLQRQAGKA
                     RRYKQLMMELQHLDTQLARHQFDVLQSEISERNLSAEKLREEIEVCSSTVLQKEDEIT
                     QLREQLSTLEHQISEMQQRGLELKGQIDRQESRIQFNEERLNELGSQNTKALADITQA
                     EERQHAAHTELGILTERLAASQAALEQHRNALQTKRDAVAAIEETLRQLQEAQRQTQS
                     ETFAQSQQLARARNEINALDLQKQGNVVRLEKLSAEKIQLEEERGQLEVRLNEFAANV
                     EVQKLNAQTQRGTVEERQARLREIHTELAQLTQNLDAQLQQQAEKRSRLNVLEQLQSE
                     HEGFSAGTLAALKSAEQVMGSLADKIRVPDQYITAIETALGHHLQLVITENPEAARNI
                     LADLSANKKGRASIASLSLRNAAPVAENVSFQPSASDAASANVTENSRFQLHPIALSV
                     IESDAAIRPLLESLLGRTLIVPDLDAATALWRESNGALDFVTLSGEVLSRHGVFTGGS
                     SNGSGKAPGSILGRKNQIAELQVQLAQAHEQVNELSRNKGALLSEQTSLQAGLQQAQT
                     ELSQQEVAIATRQGEFNALQNSQRLLHQKIDTVVYEIQSLAAQEQEGIQKRTALAQQV
                     GELEARESTLQQQLAEIAGNIENSRQQRDAATTTLTETKVALASEEQICSSFGQQQQS
                     LEQRLRELSQLVAQRRAEVSTFLSRKTQAESEILDSRGQIDSLQHDREQVNTQVAGLI
                     AQKDEQETDIAAREDELRERRRRLTECQQQRGALEIELAQKNMSVQNLRERIQQKYHV
                     NLDDVRSECITITFADEGPAKVQTLTPEEMAASGAATDWNVVGEQVTALQKRIDEMGP
                     VNLVAIEEYEETEQRHTFLTSQHDDLVSAKAQLLEVINKINLQTRQMFTETFEKIRDN
                     FRAMFTEVFGGGKADLLLVGEGDALESGIDIVARPPGKQLQTISLLSGGEQTMTAVAL
                     LFSIYQVKPSPFCVLDELDAPLDESNINRFIRVLQRFLAHSQFIIITHNKRTIGMADV
                     LYGVTMEEHGISKIVSVKFHKADESDQGATPLVPPATGAGQVEKAEDTPHKREETMDI
                     AMAK"
     gene            6866..8242
                     /locus_tag="VG754_05880"
     CDS             6866..8242
                     /locus_tag="VG754_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18775.1"
                     /translation="MAGQRKFIVALAFVIVLAAFAFFVLSRAADKRSATGQTLPDGST
                     LRLGTVQFTNGNFSYTATRLKGWQTALLRILPVKLADKLVNGRLSGGITFGSGATGTN
                     LCVFTIHEGRANSKFNNMRIQVFDDLGNTFDAGTTRGVMSTSDMINELRVEGWCPPAF
                     PRRGKMIGLRFYEWGKSNAPCLAEFTMPNPAPGNYPVWTPESLPATRNTNELTVTLVS
                     LQSGLSEARQTNAAASNEIAMTQAAFRIVQSGNGSNSWRPKEVEISDATGNHWTPFAS
                     VVSNRHEDGMDYFSFEGALWSGEATWKMRFEFSRVADFDPAEVFTFSDVVVPGATQVI
                     NLENSTNVGGSKLQLVAITGEKAKQPGNLKWSTVEDYMNVSIKAAPFPKGFRLNLLRV
                     TDDSGREAEVLRGPDWNSPERVYPFKTPKGAKQLNFTFALHKSRFVEFLAKPEYVYGG
                     DAFHGETR"
     gene            8623..>10293
                     /locus_tag="VG754_05885"
     CDS             8623..>10293
                     /locus_tag="VG754_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18776.1"
                     /translation="MLFLSVFTSRAAIQFDVFDGFDDWVPEASWFPIVCEIKNDGASF
                     NGVIEITGGQFNEGQTRRVFVELPTGTLKRIVIPVFSTASYQSSWDVRLLDERGRMHA
                     EQLNHAPHVITSATTLIGAMPRTATGVPVLRAPQGQETELKPVAARIPTALLPDNPLV
                     FEGLDCIYMNSEKAADLNEHQVFALMAWLNNGGHLIVGIEQITDVNAVPWLKSLVPCD
                     LADMKTVSSHHELQDWLRGRPSTNENNTARNKIRFQTRNRASNYNPFSDLAEDPDFEK
                     ADLQVITGTLHGGAALVSAGDTPLMIASHQGRGRVTALLFSPERKPFSNWKNLPSFWA
                     KLTEVPPDIYRPDNGNNFNANYNAGWSIDGVFGAMIDSKQVRKLPVEWLLLLLIAYLV
                     VIGPLDQYWLKRIKRPMLTWITFPCYVVMFSLLIYFIGYKLRAGETEWNELHLVDVLQ
                     AGDSAELRGHTYASIYSPINATYRVESQERYSSFRGEFQSRFMGGGQSGGERAEVLQT
                     GDNFKADIFVPVWTSQLYASDWLEAAPLPVKLDVTTDGSDWSVSVMNLR"
BASE COUNT         2482 a         2618 c         2959 g         2234 t
ORIGIN      
        1 actgagctgt gaaaacgttt tcctcgcgaa atttccaccg ggctctttcg atttcattta
       61 ttcgctcggc atgttcggtc atggctgccc gacaacctcg gagctctgca atcgttttca
      121 cgactggctt gcgccgggcg gacagctttt tttcaacgtg gtcagcctgc attcgatgtc
      181 atttgtccag cgggtcagac gaagcattcg caccaagctt tatccgttgc tcccaaggca
      241 aatcaaaaaa gcgctggatg cgcgagaggc gcgcgttccc tttttcggcc tctcagggaa
      301 agaccttgcg ggaatcatgc gcgcaagcag gtttggcgat tactcgattt cctcccatgt
      361 ttatcactcg caactttggc ggaatgttca cctcgaatgt caggccatca aatcgcggtg
      421 agggatttga cgagcctgac agttctaatt catgtcctgc ttgatgcagg ctttcaccct
      481 gagccagtcg ggcatctctt gaggagaaac cgaggtgatc caactcgtaa aagtttttgg
      541 aacgacccat ttgaaacgct caacatggcc gtcagcgaag gaaagattcg cgccttgatc
      601 atgccggttt gccggcatgt cccaccaata agtcgagcgg tcataaaaat ctgtgggcat
      661 gccaaattgc gagtcaagca gtgtgtattc attctcgtca atgaacacca ggcaattcgc
      721 gacgttggga tttctgatgt cagtggtttt gacgaagcaa ggtatgtaac cgctcaggaa
      781 ccagttgtag ccattgactg attgactcat gttgtaactt cgtgatcgca aagtcccgtt
      841 gtcgcggccc gaaggatcag tcaatgtgga atggtccgca ggacaatggt aaatgccaag
      901 cgaggtgttg tacggaaaca acatcccgtt ctcgacattg ggctccgtgg gatccgccaa
      961 gcaccatgac acaccagtcg caatcgagcc ttcccctccg ttggtcgtga tgccttccac
     1021 cgaattattg ggaacgaggt attcggaatt atccattgtg tagaggtgcc aacaagtttg
     1081 aagctggtta aggttgttca aacaggcggc ttcccatgcg cggctcttgg cgcgcgccaa
     1141 cgccggcaat aacaggctgg cgagaatcgc gatgatggcg acgaccacca gcaactcaat
     1201 taacgtgaag gctgctgcgc gaggaattct gaagggcttg ttttgccggc tgaaaacatc
     1261 ttcagcgttg ccggtgatgt tcatggacgt gaaacgaccg tttgctgcaa caggaacatc
     1321 accatgttag caaaaactgt ccgaaacgcc acttaaattt cgcactggca actcgtcctg
     1381 aagcaaaatt gtgaactctt gacactttaa ttcatccgca cgagcatgat acagcttggt
     1441 taaatcaata aaatttttag cctcttggac gatcttttcg attagcttcc tttgtgtaag
     1501 cgcctttgtt tcgggatcgt ttgttggtcc gaacctatat aactgcaccg atgatgggcc
     1561 cgtggggatt atcggatttt ctttttccag gaagggcatg ggctcataac aagcctgttc
     1621 cggttgagca tgtgtatcct gatatgaata tgaatcatcc tgataccatc aaagccggct
     1681 ggacaatcca tgggatatgg attctttggt tcacatttgt gacgatttcg tttcttattg
     1741 ccttgctggt cacaatctgt gcttggcgca ataagactca caggaggatt ttagaaacaa
     1801 ttaccaggac ttggatcgaa tgaacaagaa aagcgcgaaa atcaccgccc tgatcgaagg
     1861 ctgccgcggg caggatttgg acgcgcatta cctcggattt ttcgagtgtt tcaaccagac
     1921 tcttttttat gaagcccacg atgtgctgga agaactttgg ctggccaacc ggcacagctc
     1981 gaattactcc tttcacaaag gattaataca attggcaggg gcgttcgttc atctgcaaaa
     2041 aaatcgtctc aaacccgcag cggcgttgtt caaactcgcc cggacaaatc tccaaaaata
     2101 tcccgaacgg catgagcatc tggatgtttc cgagactctt ttgctgattg aacgctggtt
     2161 gcaggaattg gaatcgaatg atttcatcac gaatccactc aaggcgaaca acgcgccgaa
     2221 gttgcatttg ctcaaacgcg attaagattc gacagcaagg gaaaatttca tccgcataaa
     2281 atttctggaa acaaaaccat ttttcaatcc tcgactaaaa gcctccaacg agtacgggaa
     2341 tgaaaagcat atattggcgc ggagggcgga gattgccaac tgagcaagca cgtatgcaaa
     2401 atatggttga gcaggattac ccacaaaatt cacagttgtg gcaatgctgt agcctgcaaa
     2461 tgcaggggga tgagcgtcaa attgtaaaaa tatgcgacga cgctcccaat aacgaacgct
     2521 ggattttcta aatgttcgaa agtcgttcat acgattcgct ggccagccct ttttgcaaga
     2581 tggaatttgc ggttgaaagg gcttgtcgag acaaacatta tgaagcttgt ttgacgtttt
     2641 cagagtggtt tgctttattt cgttgcccgg gggtatcgtt gcgccaaaac cccggctaat
     2701 ttctggcaac ccttcgggtt gcacggaacc tcatgtagcg aatcccggga agtcacctat
     2761 cggctttgaa aataccccac aaatcggttg tttcaacatg ttaattgcgc caaattgtcc
     2821 ctcaaatggt gaataaagct gtttacaagc tgtagtgggc tgtgaataag tttgccactt
     2881 tatgtagtgg ttttctcttt actcggcagg aggttttggg catttttacc gaaaaatcag
     2941 catgtatctc aagaatttaa ctgttttggg gttcaaatcg ttcgcgaaca agaccagcct
     3001 gaacttccag ccgggcgtca cggccatcgt cggacccaat ggttgcggca agtcgaatgt
     3061 gtcggatgcc gtgcgttggg tcttgggcga gcaatcggcc aaggcgttgc gcggcggcga
     3121 aatggccgat gtgatcttca acggcacgga cggacgcaaa ccgctcggca tggcggaagt
     3181 gtcgcttacc atcggcggcg tggacgacga acatctcatg gcggcggggg tcgaggtcgc
     3241 ttacaacgag gtgacgatca cgcggcgggt tttccgcgac ggcggcagcg aatatttcat
     3301 caacaaaact ccctgccggc tcaaggacgt gcagcagctt ttcatgggca cgggcgtcgg
     3361 gcgcacgagc tacagcatca tggcgcaggg gaacatcacg caaatccttt cgagcaagcc
     3421 ggaagatcgc cggatgattt ttgaggaagc cgcgggcatc acgaagttca aggcgcaaaa
     3481 aaaggaatcg ctgcgcaagc tcgaatacac cgagcagaat cttttgcgcg tcgcggacct
     3541 catccgcgaa gtgaagcggc agatcgggtc gttgcagcgg caggcgggca aggcgcgccg
     3601 ttacaagcaa ctgatgatgg agttgcagca tctggacacg caactcgcgc ggcatcagtt
     3661 cgacgtgctt caatcggaaa tttccgaacg caacctctca gcggaaaaat tgcgcgaaga
     3721 aatcgaagtt tgttcgtcca cggttttgca aaaggaagat gaaatcacgc agcttcgcga
     3781 acaactttcg acgctggaac atcagatcag cgagatgcag caacgcgggc tggagttgaa
     3841 gggacagatt gaccgccagg aaagccgcat ccagttcaac gaggaacgcc tcaacgaact
     3901 cgggtcgcag aatacaaagg cgcttgcgga catcacgcag gcggaagagc gccagcacgc
     3961 ggcgcacaca gaactcggca ttttgacgga gcgccttgcc gcatcgcaag ccgcgttgga
     4021 acagcatcgc aatgcgctgc aaaccaagcg cgacgcagtg gcggccatcg aggagacatt
     4081 gcggcaattg caggaagcgc agcgtcagac gcagtcggag acattcgcgc aatcgcagca
     4141 gcttgcccgc gcgcgcaatg agatcaacgc gctcgatttg cagaagcagg ggaatgtggt
     4201 gcggcttgaa aaactttcgg cggagaaaat tcaacttgag gaagaacgcg gccagttgga
     4261 agtgcgcttg aatgaattcg ccgcgaacgt cgaggtgcag aagctcaatg cgcagacgca
     4321 gcggggcacg gtggaagaac gccaggcgcg tttgcgcgag atccacactg aactcgcgca
     4381 gctcacgcaa aatttggacg cgcaattgca gcaacaggcg gagaagcgtt cgcggttgaa
     4441 tgtgctggag caattgcaat ccgagcacga aggtttcagc gcgggaacgc tggccgcgct
     4501 caagagcgcc gagcaggtga tgggttcgct tgcggacaaa atccgcgtgc cggaccaata
     4561 catcaccgcg attgaaaccg cgcttggaca tcatctgcaa ctggtcatca cggaaaatcc
     4621 cgaagccgcc cggaatattc tcgcggacct gagcgcaaac aaaaagggcc gcgccagcat
     4681 cgcgtcgctt tcattaagga acgcagcccc cgtagctgaa aacgtaagtt ttcagccttc
     4741 cgccagtgat gcagcgtcag caaatgtgac tgaaaactca cgttttcagc tacatcccat
     4801 tgcgttgagc gtgattgaat ccgatgccgc gatccgcccg ctcttggaaa gtttgctggg
     4861 gcgcacattg attgttcctg atttggatgc cgcgacggct ttgtggcgcg agagcaacgg
     4921 cgcgttggat tttgtcacgc tttcaggaga agtgttgagc cgccatggtg tcttcaccgg
     4981 cggttccagc aacggttcgg gcaaggcgcc cggctcgatt ctcggacgca aaaaccaaat
     5041 tgccgagttg caggtgcagc ttgcgcaggc gcacgagcag gtgaacgaac tcagccgcaa
     5101 caaaggcgcg ctgttgagcg aacagacttc gttgcaggct gggttgcaac aagcgcagac
     5161 ggagttgagc cagcaggaag tggccatcgc cacgcggcag ggcgaattca acgcgctgca
     5221 aaattcacag cggctgttgc accaaaaaat tgacacggtc gtttacgaaa tccagagcct
     5281 tgccgcgcag gagcaggaag gcatccagaa acggaccgcg ctggcgcagc aggtcggcga
     5341 acttgaagcg cgcgaaagca cgttgcaaca gcagttggcc gagattgccg gcaatatcga
     5401 gaattcgcgc cagcagcgcg acgccgccac gaccacgctg acggaaacga aagtcgcgct
     5461 cgcgtcggag gaacagattt gttcgtcgtt cggccagcag cagcaatcgt tggaacaacg
     5521 tttgcgcgag ttgtcgcaat tggttgcgca acgtcgcgcg gaagtttcca cttttctcag
     5581 ccgcaagacg caggccgaat cggaaatttt ggattcacgc gggcagattg attcgttgca
     5641 gcacgaccgc gagcaggtga acacgcaggt cgcgggattg atcgcgcaga aggacgagca
     5701 ggaaacggac atcgcggcgc gtgaagatga gttgcgtgag cgtcgtcgtc gtctgaccga
     5761 atgccagcag caacgcggcg cgttggagat cgaactcgcg cagaaaaaca tgtcggtgca
     5821 aaacttgcgc gagcgcatcc agcagaaata tcacgtcaac ctggatgatg tccgcagcga
     5881 atgcatcacc atcacgtttg ccgatgaagg cccggccaaa gtgcagacgt tgacgcctga
     5941 agagatggcc gcgagcggcg cggcgacgga ttggaacgtg gttggcgaac aagtcaccgc
     6001 gttgcaaaaa cggattgatg aaatgggccc ggtgaatctt gtggcgattg aggaatacga
     6061 ggaaaccgag cagcggcaca cgttcctcac cagccagcac gatgatttgg tctcggcgaa
     6121 agcgcagttg ctcgaggtca tcaacaagat caatttgcag acgcggcaga tgttcacgga
     6181 aaccttcgag aagatacgcg acaattttcg cgcgatgttc acggaagtgt tcggcggcgg
     6241 gaaagccgac ttgctgctcg ttggcgaagg cgatgcgttg gaaagcggca ttgacatcgt
     6301 ggcgcgcccg cccggcaagc aattgcagac gatttcgctg ctgtccggcg gcgagcagac
     6361 gatgaccgcc gtggcgttgc tcttttcgat ttaccaggtc aagccgagtc cgttctgcgt
     6421 gctggacgaa ttggacgcgc cgctggacga atcgaacatc aaccggttca tccgcgtgct
     6481 gcaacggttc ctcgcgcatt cgcaattcat catcatcacg cacaacaagc gcaccatcgg
     6541 catggcggac gtgctgtatg gcgtgacgat ggaggaacat ggcatcagca agattgtgag
     6601 cgtgaaattt cacaaggccg atgaatccga tcagggcgcg acgccgctcg tgccgcccgc
     6661 gactggagcg gggcaagtgg aaaaagcgga ggacacaccg cacaaacgcg aagagacgat
     6721 ggacattgcg atggcgaagt gagggaaatg gagagatgga gagatggagt gatacaaagc
     6781 tggagtgatg gagtattggg tggaagggac ggcgcaactt tagcgcaagg ccggggttgt
     6841 atggagggcg cttgcaaggg ataaaatggc gggacaaaga aaattcattg ttgcgttggc
     6901 gtttgtaatt gtgctggcag cgtttgcatt tttcgtgttg agccgcgccg ctgacaaacg
     6961 ctctgcaacg gggcaaacgc ttccggatgg gtcaacgctt cggttgggaa cggtgcaatt
     7021 caccaacggc aatttttcct ataccgcgac aagattgaag ggttggcaga cggcgcttct
     7081 gagaatcctt ccggtcaaat tggcggacaa actggtcaac ggacgattga gcgggggaat
     7141 cacattcggc agcggagcca caggcaccaa tctctgcgta ttcacaatcc atgaaggacg
     7201 cgcaaattcc aagttcaaca acatgcgcat ccaggttttc gatgacttgg ggaacacgtt
     7261 tgatgctggt accacaaggg gtgtcatgag tacttcagac atgatcaatg aattgagagt
     7321 tgagggttgg tgtccgccag cgtttccgcg ccgcggcaaa atgattggac ttcgctttta
     7381 cgaatggggc aaaagcaatg cgccttgtct agcggagttc accatgccga atcccgcgcc
     7441 gggtaattac cccgtctgga cgcctgaatc gctgcccgcg acccgaaaca ccaatgaatt
     7501 gaccgtcact cttgtgagtc tccaatcggg cttgagcgag gcgcgccaaa caaacgccgc
     7561 cgcgagcaac gaaattgcga tgacgcaagc ggcattccgc atcgtgcaat ccgggaacgg
     7621 cagcaattcc tggcggccga aagaggtgga aatttcggac gcgacaggca atcattggac
     7681 gccctttgcc agcgtcgtat ccaaccggca cgaggatggg atggattatt tttcgttcga
     7741 gggcgcgtta tggtctggtg aagcgacttg gaaaatgcgc tttgagtttt cgcgtgtggc
     7801 ggattttgac cctgcggagg ttttcacttt cagcgacgtc gttgtgcccg gcgcgacgca
     7861 ggtcatcaat cttgaaaact caaccaatgt cggcggaagc aagttgcaat tggtggccat
     7921 caccggcgaa aaagccaaac agccgggcaa tttgaaatgg tcaaccgtcg aggactacat
     7981 gaacgtctcg atcaaagccg cgccttttcc aaaaggtttt cggctgaatc ttctcagggt
     8041 gaccgacgat tccgggcgcg aagcagaagt cctgcgcggt cctgattgga actcaccgga
     8101 acgcgtttat cctttcaaga cgcccaaggg cgcgaagcag ttgaatttta cctttgcgct
     8161 gcacaagagc cggtttgtcg agtttttggc gaagccggaa tatgtgtatg gcggggatgc
     8221 gtttcatggc gaaaccagat aaagaggact gtccgcctta tcgtagcgca gagttgcact
     8281 ctgccgtatc gccgacttgc agtcggcagg gcgaacaaaa tcacgaaacg cgcgaaaatt
     8341 acgagaacgt cgtgttttcg ctgtctttgc ggagtgaaac tccgcgatac agcagagtta
     8401 aactctgcgc tacgtctcac ggcaacgctc gcatggcatt ggacaaggtt gacttgcgtc
     8461 ccgatgaatg tcgtctcaca acgaattgaa cattgccaag cccaaagggt ctgcgtataa
     8521 cgagggcgca tcgagcacat gccaacaaac atgatcgaac caaggacacg gatgttccct
     8581 ttgttccaga accggggaag gcgtttattt gcatttcttt tagtgctttt tttaagcgtc
     8641 ttcacgtcac gagcggcgat ccagttcgat gtttttgatg gattcgatga ttgggtgccg
     8701 gaagcgagct ggtttcccat cgtttgcgag ataaaaaatg acggggcttc gtttaatggc
     8761 gtcattgaaa tcaccggcgg gcaattcaac gaaggacaga cccggcgcgt cttcgtggaa
     8821 ttgccgacag gcacgctcaa gcgcatcgtc atcccggttt tttcgaccgc cagttatcaa
     8881 agcagttggg acgtgcggct gctggatgaa cgcggacgga tgcacgcgga acagcttaat
     8941 cacgctcccc acgtaataac ttcggccacc acgttgattg gcgcgatgcc gcgcacggcc
     9001 actggagtgc cggtgctgcg cgcgccgcag ggccaggaga ccgagttgaa gcctgtcgcc
     9061 gcgcgcattc cgaccgcgct gctgccggac aatccgctcg tttttgaagg gttggattgc
     9121 atttatatga actcggaaaa ggccgccgat ttgaacgagc atcaagtctt cgccttgatg
     9181 gcctggttga acaatggcgg acatttgatc gttggcatcg agcagatcac cgacgtgaac
     9241 gcggtgccgt ggttgaaaag tctcgttcca tgtgatttgg ctgacatgaa aactgtttct
     9301 tcgcatcacg aactgcaaga ttggttgcgc ggaaggcctt cgaccaacga gaataatacg
     9361 gcgcgcaata aaattcggtt ccagaccaga aaccgcgctt cgaattacaa cccgttcagc
     9421 gaccttgccg aagacccgga tttcgagaag gccgatctcc aggtgatcac cgggactctg
     9481 catggtggcg cggcgctggt gtcagccggg gacacgccat tgatgatcgc gtcgcaccag
     9541 ggccgcgggc gcgtgacggc gttgcttttc agcccggaac gcaagccgtt ttcgaactgg
     9601 aaaaacctgc cgtcattttg ggcgaagctg accgaagttc cacccgatat ttaccggccc
     9661 gacaacggca acaatttcaa tgcgaattac aacgccggct ggagcattga cggcgtgttc
     9721 ggcgcgatga ttgacagcaa acaggtccgc aagctgccgg tggaatggct gctcctgctg
     9781 ctcatcgctt atctggtcgt catcgggccg cttgatcaat attggttgaa acgcatcaag
     9841 cgtcccatgc tgacgtggat cacctttccg tgctacgtgg tgatgttttc gctgctcatt
     9901 tatttcatcg gctacaaatt gcgcgctggc gagaccgagt ggaacgaatt gcacctggtg
     9961 gatgtgttgc aggcgggcga tagcgcggag ctgcgcggcc acacttacgc atccatttat
    10021 tctccaatca atgcgacgta tcgcgtcgaa tcgcaggagc gttattcatc gttccgcggc
    10081 gaattccaaa gcaggttcat gggcggagga cagagcggcg gcgaacgcgc cgaggttttg
    10141 cagacgggtg acaatttcaa agcggacatt ttcgttcctg tctggacgag ccagctttac
    10201 gccagcgatt ggctggaagc cgcgcccttg cctgtgaagc tggacgtgac caccgacggc
    10261 tcggattggt cggtgtccgt catgaacctc cgc
//
LOCUS       DASZMM010000246         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1480878, whole genome shotgun sequence.
ACCESSION   DASZMM010000246 DASZMM010000000
VERSION     DASZMM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480878"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VG754_05890"
     CDS             complement(<1..901)
                     /locus_tag="VG754_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18777.1"
                     /translation="MLLLLVVIITILLGGAFLPGRTLFSNDGPLGRLMSQCHRLPDAF
                     HGVWEDLNSVGYREQGALPNITDGLLLLLHPILLSKFYAAIAILILGLSAWCFFRQLG
                     LGPAACILGGLAAALNSDFFSEACWGVASHPITVGLAFFAMAALVKTSTWRSWLYALL
                     AGAAVGMGLAEGADVGAIFSIYVAAFAIYQAWIAEGPRVKNLALSAGKLAVVAGFAAL
                     VSAQAIFVLVNTQIEGVTGMSQDEQTRESRWQWATRWSLPKREALGIVVPGIFGYRLD
                     TTNGGYYWGALGRSPAWEKYLANG"
     gene            complement(955..2004)
                     /locus_tag="VG754_05895"
     CDS             complement(955..2004)
                     /locus_tag="VG754_05895"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWF18778.1"
                     /translation="MRKPNFFLVGAPKCGTTAMAQYLAVRPDIFMARKEMHTFGKDLY
                     FGPQFYRRNLNAYLAEFQDWGDQPRAGEASVWYLFSKTAAAEIKAFNPDAQIIIMLRD
                     PVEMLHSMYYTFVWDGNEHLPTFEAALAAENQRRTGKLTTRQTYFPQGLVYHEIVKFT
                     EQVRRYFRVFGREKVHVVIYDDFAVDVAATYRRALDFLGVNSTRVETEFEPVNANKFV
                     KSSALRALMSDPLVRSTLVAIRPMLPKAIFGVMQNVDARLRKINSRDGVRPALSPELR
                     ERLNEEFAPEVAHLSELLGRDLTHWSRSTTLPVKLKLPERTPAPVTIASPSDCAEAVQ
                     NLSLPAGEMRLDAYP"
     gene            2480..3499
                     /locus_tag="VG754_05900"
     CDS             2480..3499
                     /locus_tag="VG754_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF18779.1"
                     /translation="MSSLLKVAVVGTGSLGKEHVRIYAELARASRVELAGVFDSSADT
                     AQKIAAKHSVRAFASVAEAANAADALSVVTPTNTHFEIAKTLLQQGKHLLVEKPMTDN
                     AAQAAELVQLAQKHRCVLQVGHIERFNPVFKYLETIATDPRFIEIHRLSPYPGRSTDI
                     GVVLDLMIHDLDVVMALVKSPVKSVDAVGIPVLSKTEDIANARLRFENGCVANLTASR
                     VSPERMRKIRVFSGGAMPSHVSLDYRAQEGFIYRIARDGEEESSLLKKLLAAKDSTII
                     SKFGGKRIVREPVPIEKAEPLKVELQSFVDCVHARQTPVVSGESAKLALDVAFEITRQ
                     IEQIR"
     gene            complement(3636..3782)
                     /locus_tag="VG754_05905"
     CDS             complement(3636..3782)
                     /locus_tag="VG754_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18780.1"
                     /translation="MDTEPAHLLSLRPRVFAPLRFIREFSAIIAEKFPLNSAYFHPVP
                     LDSA"
BASE COUNT         1160 a         1342 c         1191 g         1003 t
ORIGIN      
        1 ggccattggc gagatatttt tcccatgcgg gactgcgtcc caacgcgccc caataatacc
       61 cgccgttggt cgtatcgagg cgatatccga aaatgccggg aacgacgatc cccagcgcct
      121 cgcgtttggg caagctccag cgcgtcgccc attgccaacg ggactcgcgc gtttgctcgt
      181 cctgggacat gccggtcacg ccttcaatct gcgtgttcac caagacgaat atcgcctggg
      241 ccgacaccaa tgccgcgaat ccagcgacca ccgcaagctt gccagcgcta agcgcgagat
      301 tttttacgcg cggtccttca gctatccacg cctgatatat cgcgaacgcc gccacataaa
      361 tgctgaagat cgcccccacg tccgcgcctt ccgcaagccc cattccaaca gcggctccgg
      421 cgagcaacgc gtatagccag gagcgccatg tggaagtttt caccagcgcg gccatcgcga
      481 agaacgccaa acccacggtt atcggatgcg acgccacgcc ccagcaagct tccgaaaaaa
      541 aatcggaatt cagcgccgcc gccaaacctc ccaaaatgca agctgcgggc ccaagtccta
      601 attgccggaa gaagcaccac gcgctcaagc ccaaaatcaa aatggcgatc gccgcataaa
      661 atttggaaag gagaatcggg tgcaacagca gcagcaatcc gtcggtgatg ttcggcaatg
      721 ccccctgctc gcgataaccg acactgttca aatcttccca gacgccatga aacgcatccg
      781 gcaaacgatg gcattgggac atcaaccgtc ccagcgggcc gtcattggaa aacaaagtgc
      841 gaccgggaag aaatgctcct cccaaaagaa tcgtgatgat cacgaccagc agtaaaagca
      901 tcacggcaaa tccagggccg cgcgattgct cccttgaaac aacagacttg aaattcatgg
      961 ataggcgtcg agccgcatct caccggcagg cagggaaagg ttctgaacgg cttcggcgca
     1021 atcgcttggc gaagcgatgg tcaccggcgc gggcgttcgt tccggcaact tcagtttgac
     1081 gggcaaagtt gtgctgcggc tccaatgtgt caggtccctg cccagtaatt cgctcaaatg
     1141 cgcaacttcc ggggcaaact cctcgttcaa ccgctcgcgc aattccggag acaacgcagg
     1201 ccgcacaccg tcgcggctgt ttattttgcg caaccgcgcg tccacattct gcatcacccc
     1261 gaaaatcgct tttggcaaca ttggccggat cgccaccaat gtcgagcgaa ccagcggatc
     1321 gctcatcaac gcgcgcaacg cggaagactt cacaaacttg ttcgcattga ccggctcgaa
     1381 ttcggtttcg acgcgtgtcg aatttactcc gaggaaatcc agggcgcgac gatatgttgc
     1441 ggcgacatcc accgcaaaat catcgtaaat cacgacatgc accttttcgc ggccaaagac
     1501 ccggaaatag cggcggactt gttccgtgaa cttgacgatt tcgtgataga ccaatccttg
     1561 cgggaaataa gtctggcgcg tggtcaattt tcccgtgcga cgctggttct ccgcggccaa
     1621 cgccgcttcg aacgtgggca gatgctcatt cccgtcccac acgaacgtgt aatacatcga
     1681 gtgcagcatc tccacagggt cgcgcagcat gatgatgatt tgcgcgtcgg gattgaaggc
     1741 cttgatttcg gcggcagcag ttttggaaaa gagataccag accgaagctt caccagcgcg
     1801 cggctggtca ccccaatctt gaaattcggc aaggtaggca ttgagattgc ggcgatagaa
     1861 ttgagggcca aaatacaaat ctttgccgaa cgtgtgcatc tccttgcgcg ccatgaaaat
     1921 atcaggccgc accgccaagt attgcgccat cgcggtggtg ccgcacttgg gcgcgcccac
     1981 cagaaaaaaa ttaggcttcc gcatttgtcg taagtctctg tgccagccag acatcgttgc
     2041 gcgatttgcg ccgccatttt gaaggcttgt gggtgattca aactgttttg aaaaccgcca
     2101 aggcttcaga aaagaccgga tgtccggcag gcttgggatt aaaaatgcgg aatgtccttt
     2161 ggagaccgac ccagctttct cagattcata tttgcctgcg ctttctataa actcaatcgg
     2221 tttactgctc gattgaagtt gccctatcgt gctcccgctc ctccgttcat gcaagtaaaa
     2281 aagtggaaat tccggcggca atgggcgcat ctcagcatct ggcacgacgg ggttcccccg
     2341 atttagtggg caaagcgggg aaccccggcg atgtccgcaa atcaattcgc tcaagctgcc
     2401 aacaacttcg caaccgccaa ttcgcgcaac cataacttta aactttaaac attgaacttt
     2461 aaacgcgtat aaaatgccca tgtcatcatt gctcaaagtc gcggtcgtgg gcacaggctc
     2521 gctcggcaag gagcacgttc gcatttatgc cgaacttgcc cgcgccagcc gcgtcgaact
     2581 cgccggcgtt ttcgattcgt ccgccgacac cgcgcaaaag attgccgcca aacattccgt
     2641 gcgcgcgttc gcgtctgtcg ccgaagccgc caacgccgct gacgccttga gcgtcgtcac
     2701 gccgacgaac acccatttcg aaattgcgaa aactctcttg cagcaaggca aacatctgct
     2761 cgtcgaaaaa ccgatgaccg acaatgccgc gcaagccgcc gaactcgtcc aactcgcgca
     2821 aaaacatcgt tgcgtgttgc aagtcgggca catcgagcgg ttcaatcccg ttttcaaata
     2881 tctcgaaacc atcgccaccg acccgcgctt catcgaaatc catcgccttt cgccttaccc
     2941 cggccgcagc accgacatcg gcgtggtgct cgatttgatg atccacgatt tggacgtcgt
     3001 catggcgctc gtcaaatcgc ccgtgaaaag cgtcgatgcc gtgggcatcc cggtcttgag
     3061 caaaaccgag gacatcgcca acgcgcggtt gcgattcgag aacggctgcg tggcaaacct
     3121 caccgccagc cgcgtcagcc ccgagcggat gcggaagata cgcgtgttca gcggcggcgc
     3181 gatgcccagc cacgtctcgc tcgattaccg cgcgcaggaa ggtttcattt atcgcatcgc
     3241 ccgcgacggc gaagaggaat cttcgcttct caaaaaactg cttgcggcca aggattcgac
     3301 catcatcagc aaattcggcg gcaaacgcat cgtgcgcgag ccggtgccca tcgagaaagc
     3361 cgagccgttg aaagtcgagt tgcaaagttt tgtggattgc gttcatgcgc ggcaaactcc
     3421 cgtcgtcagc ggcgaatccg ccaaactggc cctggatgtc gccttcgaga taacgcggca
     3481 gattgagcaa attcggtgaa gggaatgacc gcgcgttgaa acgcaaagac gcaaagacac
     3541 caggacgcga agaatgggac ggatttgcgg ttgttgggta tcccttgcgt ctttgcgtta
     3601 aaggccgagt ttcatggctg gaaaaattta tttttttatg cggaatcaag cggaacgggg
     3661 tggaaataag cggaattaag cggaaatttc tccgcgatga tggcggaaaa ttcgcggatg
     3721 aaacgcaaag gcgcaaagac gcgagggcgc agagaaagga gatgggccgg ttcggtgtcc
     3781 atgctttagc atgttccccg cggttgtgcc gaagcacgct aaagcgtgga caccgaacat
     3841 ccgctctgat tgcacgtatt atatgcagcg cattttgcat cattttatct ctcccttttg
     3901 cgactctgcg cctttgcgtc gatggctgga tttcacgtta aaaaatttat tttttatccc
     3961 ctttatctga aaataactga aaataagtga aaataactga aaatagctga aaatagctga
     4021 aacgatctga ataaataagt gaaacaatgc ctcgaatgca gcccaaaagc tgaaacgctc
     4081 tgtgctccga aggaaccgtt tggtgtccat gctttagcat gtcctttcca accgctcgca
     4141 ccctaaaggg tggacaccga gcaatcggaa ttagctgaaa acacctgaaa cgatctgaat
     4201 aaataagtga aacaatgcct cgaatgcagc gcaaaagctc aaacgccctg tgcttcgaag
     4261 gagccgttcg gtgtccatgc tttagcatgt ttcttccagt ttgcgcggtc gcagacacgc
     4321 taaagcgtgg acaccgaaca tccgcccgga ttgcgtcctt aggaaccgtt tggtgtccat
     4381 gctttagcat gtcctttcca acggctcgca ccctaaaggg tggacaccga acaatcgcaa
     4441 tccatccctc catttctccc ttacccctcc atcccaatgc tcgattttca tcgttcatgt
     4501 gaccagatta ccatgcccac cttcaaaaaa tcaaacctac ggtaggtttg acacgatttt
     4561 ttttaattat gaaagattgt gaaaaaaagc gggtttggac caatgcctat gcggatttct
     4621 cgtttttgaa aaagacgtgg aattttgggt gatgaaaaaa acttttttcc ctacaaagtt
     4681 tcttggctaa ttttcg
//
LOCUS       DASZMM010000247         8646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_381235, whole genome shotgun sequence.
ACCESSION   DASZMM010000247 DASZMM010000000
VERSION     DASZMM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381235"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..446)
                     /locus_tag="VG754_05910"
     CDS             complement(243..446)
                     /locus_tag="VG754_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18781.1"
                     /translation="MSVESNPAALARGKIVEVPGSEFGVPGWENASSRPAGTAFSNPE
                     GVVSKNCYDRVGLTDYLYRSYGA"
     gene            483..776
                     /gene="groES"
                     /locus_tag="VG754_05915"
     CDS             483..776
                     /gene="groES"
                     /locus_tag="VG754_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWF18782.1"
                     /translation="MALNVKPLGDRILVEPVEEKETKKGGIIIPDTAKEKPMESMVVA
                     LGTGKTDDNGKKVPFEVKKGDRVLVSKYGGTEIKLDGKEYKILNSDDILAVIE"
     gene            820..2463
                     /gene="groL"
                     /locus_tag="VG754_05920"
     CDS             820..2463
                     /gene="groL"
                     /locus_tag="VG754_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWF18783.1"
                     /translation="MAAKQLVFDEAARQSLLRGVEKLSRAVKATLGPKGRNVVLDKKF
                     GSPTVTKDGVTVAKEIELEDAYENMGAQMVREVASKTSDTAGDGTTTATVLAESIYKE
                     GLKNVTAGASPISLQRGIQKAVDAAVEHLAKIAKKVKDKEEIKQVATVSANWDNTIGE
                     IIADAMDKVGKDGTITVEEAKSIETTLEVVEGMQFDKGYLSPYFVTNAESMEAKIEDA
                     YILIYEKKISSLKDLLPVLEKVAKVGRPLLIIAEDVEGEALATLVVNKLRGTINVCAV
                     KAPGFGDRRKAMMEDIAILTGGKCITEDLGIKLENVQLEDLGKTKRVTIDKENTTIVE
                     GYGKPGEIQGRVNQIRRQIEETTSDYDREKLQERLAKLAGGVAVINVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRCLGAIEAIKAEGDEQIGVDIVKRAVESPL
                     RCLAQNAGVEGSIVVQEVKKRKGNDGYNVATGEYEDLVKAGVVDPKKVTRSALQNAAS
                     IAGLLLTTECLITELPEKEKAAPAGGGHGPGGGMGGMDY"
     gene            2951..3991
                     /locus_tag="VG754_05925"
     CDS             2951..3991
                     /locus_tag="VG754_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF18784.1"
                     /translation="MKDIPIPKFLGAILVLCFGVVFCNASPQAGVATPYTITGKVTTT
                     NGTPLADVVVFATTYINGTNYEAVNGVTGSNGMYTVHVTGNSEWFLTPDCYQNSNNLR
                     NQGYLCLDSEYIKIGSTNGSANFATVPYSDVIDELDGTVFDADQNPFAGVTVYAVNPA
                     TGETIQTTTDVNGFYYFFVLDETSWNLHVDCDQLSIKGYSCPGTITNAIIRDRNTCCQ
                     NFTVQPGAPTFFTGEVALDTPGWYWLGPQGSDTNGFGYFSAEYAPYIFHQDLGWEYFI
                     DAANAGHGAYFYDFTDDVWWYTETGLFPYMYDFNLKVWVFCAPQNGKTDSYQSNPRWF
                     LNTSTKQWVNDL"
     gene            complement(4121..4792)
                     /locus_tag="VG754_05930"
     CDS             complement(4121..4792)
                     /locus_tag="VG754_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HWF18785.1"
                     /translation="MLDARDIVLAFGTGLASGLILSIPVGPVNVTIMNEGARRGFRYA
                     MLVGIGASVMEMIYCSIAFTGFADFFNHDPVKAIMEVFSFLFIMFLGFKFLMNKTPHG
                     PGKVSRIEEKFHPHSAFMTGFVRVMGNPGILGGWIVLSASFLAHDLVQPTWSGKTACV
                     LGIGCGTNLWFCGLSYGVSLGRKKFSDTTLMWMERGSGIGLLLFGFGYGCRIVWQLAK
                     ARHGM"
     gene            complement(4912..5775)
                     /gene="pssA"
                     /locus_tag="VG754_05935"
     CDS             complement(4912..5775)
                     /gene="pssA"
                     /locus_tag="VG754_05935"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWF18786.1"
                     /translation="MEPVPLNGPEPEPPKLKIYFLPNLFTAANLFCGFLALTKIVNVD
                     ANDLASGNFKQIYNALILILAACIFDLFDGRIARMGGRESPFGREFDSLADMISFGVA
                     PAFLVQRIVLREVFANHPEIGWFIASIYVICGAFRLARFNCLAAMAGSGGGKEFLGFP
                     IPSAAGLVASLTLLMLWIYEKDFTTGFWKYFLPVLMLFLSWMMVSEVKYPTFKGLDLR
                     ATRTFTKTLIAILFIGSVIILQEKILVIVLPVFFTSYLVYGFIRPHISRKMRREIEEE
                     DEEDNGESTAV"
     gene            complement(5785..6471)
                     /locus_tag="VG754_05940"
     CDS             complement(5785..6471)
                     /locus_tag="VG754_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HWF18787.1"
                     /translation="MKHSGRARKSALKLILWTLVALGILFIAGPVAISLGVLISSLSV
                     FFLVAWVIFAAFTLYFFRDPNPRVPNEPNLIVSPAHGKVDAIDEIAELQFMGGPCKRV
                     STFLSVVDVHVQNAPVSGKVILYKYTVGEFLNALKAESAVHNENAFIGLESAERPGTK
                     IGVRLISGVIARRIVPFVNAGDDIIRGERISLIQFGSRVDLYLPLSAKVKVKIGDHVV
                     GGETIMATFD"
     gene            complement(6515..7135)
                     /locus_tag="VG754_05945"
     CDS             complement(6515..7135)
                     /locus_tag="VG754_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18788.1"
                     /translation="MKSPRGKNFILFSKPARVQKNIFPDSVMKSDEKNSKTIATLFAQ
                     NGNAFLFAQNPFVKIPDEKPPLFLSCSLALFWDSALLRLRRYFARFWALADYVYGPAH
                     IAVPLIPAFSPAGTMQLSHGLNTDETRTGGENSHFDNSKSTRQMVTAFPALHNGSACI
                     SPCSIRVSSVAELNGYDRGEGLGEGERQQTVLVHSQEQCQEAPQSL"
     gene            7289..7855
                     /locus_tag="VG754_05950"
     CDS             7289..7855
                     /locus_tag="VG754_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18789.1"
                     /translation="MKPFIWSYLSLVALSVSLIIPKANAIPMEIPGPGMDIPKNGTGP
                     GGGNGVSPSDDFFRLQNIVLAYDLAHPLTPLPTPIFTGNESLSSPDVGSGGLTGFDYA
                     VLHYGVGNGGVPASGGGIEIFYLNGASSFDFPANGSGPNGLGGFSGLVLFEGTPPSLA
                     PDGGMTLGFLAVGIGCLAVARRFTHAKA"
     gene            complement(8203..>8646)
                     /locus_tag="VG754_05955"
     CDS             complement(8203..>8646)
                     /locus_tag="VG754_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18790.1"
                     /translation="APILVIERLLSASPPLSLLLEQAGIHNHLIVLPDADSATRYLEE
                     VGTNADCQENPLPVLALWDVTLPNHYAPETGPWLTMPQQRHLVIADLRGEGLHRQFGS
                     AVDFGSNGVLVKFSSSETLLAAFRKLSRALHEGNMPDFSIALRAA"
BASE COUNT         2145 a         2310 c         2389 g         1802 t
ORIGIN      
        1 tgggatgttt tctgaaacac cgccatttat gtcgctccta cggagcttgg ctgctttttt
       61 agatgcgcat tctacaaata ggccgctcct aacggagctt gggaagacgc tcgttccatc
      121 cgaaagctcc atcaggcgca attctgtcac tgattttgaa cgggccgggc aagggcagac
      181 gagcattcca tccgaaagct ccgttaggag cgttatattt gtagcatcgc ccgaatcgga
      241 gattaagctc cgtaggagcg atataaataa tccgtcaacc cgacgcgatc gtagcaattt
      301 tttgacacaa ccccttcggg gttggaaaat gccgtgcctg cgggacggga tgacgcgttt
      361 tcccaacccg gaactccgaa ctcggaaccc ggaacttcaa cgattttccc gcgcgcgagt
      421 gcggcgggat ttgattcaac tgacatcaaa caactagaac cgaacataag gaaaaaacaa
      481 atatggcact gaacgttaaa cctctcggcg atcgcatcct cgtggagccc gtcgaggaaa
      541 aggaaaccaa aaaaggcggc atcatcatcc cggacaccgc caaggaaaag ccgatggaaa
      601 gcatggtagt cgccctcggc accggcaaga cggatgacaa cggcaagaaa gtcccctttg
      661 aagtcaagaa gggcgaccgc gtcctcgtca gcaaatacgg cggcacggaa atcaaattgg
      721 acggcaagga atacaagatc ctcaattcgg acgacatcct ggccgtgatc gaataacgaa
      781 aatccctcaa cccgcaacaa ttaacactca agaaataata tggcagcaaa acaattagtg
      841 ttcgatgaag ccgcgcggca gtccctcctt cgcggagtgg aaaaactcag ccgcgccgtc
      901 aaggccactc tcggtcccaa gggccgcaat gtggtgctcg acaaaaaatt cggctcgccc
      961 accgtcacca aggacggcgt gacggtcgcc aaggaaattg aactggaaga cgcctatgaa
     1021 aacatgggcg cgcaaatggt ccgcgaagtc gccagcaaga ccagcgacac cgccggtgac
     1081 ggcacgacga ccgcgaccgt tctcgccgag tccatctaca aggaaggctt gaagaacgtc
     1141 accgcgggcg ccagccccat cagcctccag cgcggcatcc aaaaggccgt tgacgccgcc
     1201 gtcgagcacc tcgccaagat cgccaagaag gtcaaggaca aggaagaaat caagcaggtc
     1261 gcgaccgttt ccgcgaactg ggacaacacc atcggcgaaa tcatcgctga cgccatggac
     1321 aaggtcggca aggacgggac catcaccgtt gaagaagcca agtccatcga aaccacgctt
     1381 gaagtggtcg aaggcatgca gttcgacaag ggttatctct cgccttactt cgtgaccaac
     1441 gccgaatcca tggaagcgaa gatcgaggac gcctacatcc tgatttacga gaagaaaatc
     1501 agcagcttga aggacctgct cccggtgctc gaaaaggtgg ccaaggtcgg ccgtcctttg
     1561 ttgatcatcg ctgaagatgt cgaaggcgaa gcgctcgcga ccctggtcgt gaacaagctg
     1621 cgcggcacca tcaatgtttg cgcggtcaag gcccccggct ttggcgatcg ccgcaaagcg
     1681 atgatggaag acattgccat cctcaccggc ggcaaatgca tcactgaaga tttgggcatc
     1741 aagctcgaga acgtccagct cgaagacctc ggcaagacca agcgcgtgac catcgacaag
     1801 gaaaacacca ccatcgttga aggttacggc aagcccggtg aaatccaggg ccgcgtcaac
     1861 cagatccgcc gtcagatcga ggaaaccacc tcggattatg accgcgaaaa attgcaggaa
     1921 cgcctggcca agctcgccgg cggtgtggcc gtcatcaatg tcggcgcagc gaccgagacc
     1981 gagatgaagg aaaagaaggc gcgcgtcgag gacgcgttgc acgcgacccg cgcggctgtc
     2041 gaggaaggca tcgttcctgg cggcggcgtg gcgttgatcc gctgccttgg cgcgattgaa
     2101 gccatcaagg ccgaaggtga cgaacagatc ggcgtggaca tcgtgaagcg cgcggttgaa
     2161 tcgcccttgc gttgtttggc gcagaatgcc ggcgtggaag gctccatcgt cgtgcaggaa
     2221 gtcaagaagc gcaaaggcaa cgacggttac aacgtcgcca ccggcgaata cgaagacctc
     2281 gtcaaggctg gcgtggttga cccgaagaag gtcacccgtt cggcgttgca aaacgcggct
     2341 tcgatcgcgg gcctgttgct gacgaccgaa tgcctcatca ccgagttgcc ggagaaggaa
     2401 aaagcggctc ctgcaggcgg cgggcacggt cctggcggcg gcatgggcgg catggattac
     2461 tgagctgacc ggcaccggat atccggccag catcagagac agaggaaccg agcagaaaac
     2521 gcaacggggc tgggtaacac cagccccgtt tttttaggaa aaaagggtaa catcgaacgc
     2581 tgaacactga acgccgaaca tcgaatgaaa aaccggttgc ggtcccaatt cggcgttcga
     2641 tgttcgatgt tcagcgttcg atgtttccgt tcgtgggctg ttgtgaccga ttattatacc
     2701 actgaaggga gagtggtggc attctttcaa tcatccccgc cgcacaccag cttgcgaagc
     2761 catcattgca aaaagcaaaa aatattgatc ttcaatcgac aaaatgcgaa gccggattat
     2821 ccgtacgagc ctgtacaggc ttggcacatc tcttgcagac ctgtaaacgc tttttgagat
     2881 ggccttgatc ggtgatcgat gaagccacgc tggactcgga ggcaatttaa cccttaaacg
     2941 taaatatatc atgaaagaca ttcccatccc caaattcctt ggtgcgattc ttgtgttgtg
     3001 tttcggcgtc gtgttttgca acgcttctcc gcaggcaggt gtcgccacgc catatacgat
     3061 taccggcaag gtcacgacaa cgaacggcac tccccttgct gacgtcgttg tattcgccac
     3121 aacgtatatc aacgggacca attatgaagc cgtaaacggc gtgaccggca gcaacggaat
     3181 gtacaccgtt cacgtcaccg gaaattctga gtggtttctc acgccagatt gttaccaaaa
     3241 cagcaataac ctgcggaacc aaggctacct ttgcctggac tccgaatata ttaaaattgg
     3301 ctcaaccaat ggctcggcca actttgcgac ggtgccttac agcgatgtga tagatgaatt
     3361 ggacggcact gtttttgatg ccgatcaaaa tccatttgcg ggcgtgaccg tctatgccgt
     3421 caacccagcc actggagaaa cgatccagac aacgacggat gtcaacggct tctattattt
     3481 ttttgttttg gatgaaacat cctggaatct tcacgtggat tgcgatcagc tctccattaa
     3541 gggctatagt tgtcccggca caatcaccaa tgccataatc cgtgaccgga acacttgctg
     3601 ccaaaacttc accgtccaac ccggcgcgcc tacattcttc accggcgagg tcgcgctaga
     3661 tactcccggt tggtactggc tggggcctca aggttcagac accaatggct tcggatattt
     3721 cagcgcggaa tacgcccctt acatcttcca ccaggacctt ggctgggaat atttcatcga
     3781 tgccgccaat gctggtcatg gcgcttattt ctacgatttc accgatgacg tctggtggta
     3841 caccgagacg ggtctgttcc catatatgta tgatttcaac ctgaaggtct gggtgttttg
     3901 cgctccgcaa aacggcaaga ccgacagtta tcaatccaac ccgcgttggt tcctgaacac
     3961 ttcaaccaag caatgggtga atgaccttta agcacgggta aaacaaatcg ttcacggcct
     4021 gcgtcccatt ttccgaattc aagaccagaa gcgtcgccaa gcctttcgtg ttagagccgg
     4081 cttgcgtcgg ctgctacttc aattctgtag catcacgttt tcacatccca tggcgcgctt
     4141 tggcgagttg ccacacgatg cggcaaccgt agccaaatcc aaatagcaac agcccgatgc
     4201 ccgaaccgcg ttccatccac atcaaggtgg tgtcgctgaa ttttttgcgg ccaagcgaga
     4261 cgccgtagct caatccgcaa aaccaaaggt tcgttccgca tccgatgccg agcacgcagg
     4321 cggttttacc tgaccacgtg ggttgcacga ggtcatgcgc gagaaaactt gcgctcaaaa
     4381 ctatccagcc gccgaggatg ccgggattgc ccatcacgcg cacgaacccg gtcatgaacg
     4441 ccgagtgcgg atgaaatttt tcctcgatgc ggctgacctt gcccgggcca tgcggcgttt
     4501 tgttcatgag aaatttgaac ccgagaaaca tgatgaacaa aaagctgaag acttccatga
     4561 tggcctttac gggatcgtga ttgaagaagt ccgcgaaccc ggtgaacgcg atggagcaat
     4621 aaatcatttc catgaccgac gcgccgatgc cgaccagcat ggcgtatctg aatccgcggc
     4681 gcgcgccttc gttcatgatg gtgacattca ccgggccgac gggaatggaa agaatcaatc
     4741 cgctggccag gccggtgcca aaggcgagga cgatgtcgcg cgcgtcgagc atggggttcg
     4801 ttacatggtg aaaaaagcgt tacatggttg catggttaca tggacggaat acttcgctcc
     4861 cgtaaatttg taacgatgta accatttaac cttgtaacca ttcaaccact ctcacaccgc
     4921 cgtggactcg ccattatctt cctcgtcctc ctcttcgatt tcccggcgca tcttgcgcga
     4981 gatgtgcggg cggatgaacc cataaaccag atacgaggtg aagaacaccg gcaacacgat
     5041 gaccagtatc ttttcctgca aaataatgac gctgccgatg aacaagatcg cgatcagcgt
     5101 tttcgtgaac gtgcgcgtgg cccgcaggtc gagtcccttg aacgtcgggt acttgacttc
     5161 gctcaccatc atccaggaaa gaaaaagcat cagcaccggc aaaaaatatt tccaaaagcc
     5221 ggtggtgaaa tctttttcat agatccagag catcagcagc gtgagcgacg ccaccaaacc
     5281 cgccgcggat gggattggga agccgaggaa ttccttgccg ccgccgcttc cagccatcgc
     5341 cgcgaggcaa ttgaaccgcg ccagccggaa cgcgccgcag ataacgtaaa tggacgcgat
     5401 gaaccagccg atttccggat gatttgcgaa cacttcgcgc agaacgatgc gttgcacgag
     5461 aaacgcgggc gcgacgccga acgaaatcat gtcggcgagc gaatcgaatt cgcggccaaa
     5521 gggactttcg cgtccgccca tgcgagcgat gcgtccgtca aacagatcaa agatgcacgc
     5581 ggcgaggatg aggatgagcg cgttgtagat ttgcttgaaa ttgccggaag ccaggtcgtt
     5641 ggcatcgacg ttcacgattt tggtgagcgc gaggaagccg cagaagaggt tcgccgccgt
     5701 gaaaaggttc ggcaggaaat aaatttttag cttgggcggt tcgggttcag gcccgttgag
     5761 tgggacaggc tccatagcgc attgttaatc gaaagtggcc atgatggttt cgccgccgac
     5821 gacgtggtcg ccgattttga ctttgacctt ggccgagagc ggcaggtaaa ggtccacgcg
     5881 cgaaccgaat tggatgaggc tgatgcgttc gccgcggatg atgtcatcgc ccgcattcac
     5941 gaagggcacg atgcgccgcg cgatgacgcc ggagatgagc cgcacgccga tttttgtgcc
     6001 aggccgttcg gcggattcaa ggccgatgaa ggcattctcg ttgtggacgg cggactcggc
     6061 cttcagcgcg ttgagaaatt cgccgacggt atatttgtaa aggatgacct tgccgctgac
     6121 gggagcgttc tgcacatgga cgtccacgac ggagaggaac gtggaaacgc gcttgcatgg
     6181 gccgcccatg aattgcagct cggcgatttc gtcgatggca tccacctttc cgtgcgcggg
     6241 cgagacgatg aggttaggct cattcgggac gcgcggattg gggtcgcgaa agaaatagag
     6301 tgtgaacgcc gcaaatatca cccatgcgac aaggaagaaa accgacagcg agctgataag
     6361 aacgcccagg gaaattgcga ccggccccgc gataaacaat atgcccagcg cgacaagggt
     6421 ccagaggatt agtttcaggg ccgacttgcg ggcccttccc gagtgtttca tggccataat
     6481 ttagagtgcc ttgcataaag cgcaaaggta aagttcagag tgattggggt gcttcttgac
     6541 actgttcttg agaatgtaca agtacggttt gttgacgctc gccctcaccc aaaccctctc
     6601 ccctatcgta accattcaat tcagccacag atgaaacacg gattgaacac ggagaaatgc
     6661 aggcagagcc attatgcagg gcaggaaagg cggttaccat ttggcgagtg ctttttgaat
     6721 tgtcgaaatg gctgttctcc cctcccgtcc gtgtttcatc agtgttcaat ccgtggctca
     6781 actgcatcgt tccggcaggg gagaaggctg ggatgagggg gacagcgatg tgtgctggcc
     6841 cataaacata atccgcaagg gcccagaaac gtgcaaaata acgccgaagg cgcaggagag
     6901 cggagtccca aaaaagcgca agagagcaac tcaggaataa tggcggcttt tcgtcgggaa
     6961 tttttacaaa aggattttgc gcgaaaagaa acgcgttacc attttgcgca aagagagttg
     7021 cgattgtttt tgaatttttt tcgtcggact tcattacaga atccgggaaa atatttttct
     7081 gaacgcgcgc tggtttggaa aataaaataa aatttttccc acgaggtgat ttcaaccact
     7141 tatggatttc aaacgccgcc gacgcgaggc gatttgattt tcggcacgtg ccatgctatt
     7201 gataattgag ttttacaagg tggtatcaag gcctttgcct ggactctaac tggccgtcag
     7261 cgcgaagcag cctgttcaat atcacatcat gaaaccattc atctggtcgt acctttcgtt
     7321 agttgcattg tcggtcagcc tgatcatccc gaaagcaaat gcgattccca tggaaattcc
     7381 cggcccgggc atggacattc ccaaaaacgg caccgggcct ggaggcggca acggcgtcag
     7441 ccccagcgat gatttcttcc ggctgcaaaa catcgtcctt gcttacgatc tcgcacatcc
     7501 gctcactcca ttgccgacgc cgattttcac cggcaacgaa tctttgagtt cacccgacgt
     7561 gggaagcggc gggttgacag ggtttgatta tgccgtgctg cattacggag tgggaaatgg
     7621 aggcgtgccg gccagtggcg gcggcattga aattttttat ctcaatggcg caagcagttt
     7681 tgattttccc gccaacggca gcggccccaa tggccttggc ggattctcag gcttggtgct
     7741 tttcgaaggc acaccaccca gcttggctcc ggacggagga atgacgttgg gttttttggc
     7801 agtgggaatt ggctgtctgg cggttgcgcg gcgatttacc catgcgaagg cctgaatctg
     7861 ctgacaatca tgagcactga tgggcggatt cgagaaaaat ccgcccgttc aacgacacgc
     7921 cgtgaattgt cgcgcggatt catttcatcg ttttggccca aacttttttg atggaaatca
     7981 ccctgcaagt gtagcgttca agaaaatgcg aatgaagaaa aatttgatgg atcgaattta
     8041 cccggcgcat ccgcgcagcc ccggagcgcg ggtcggtgac ccgcagcaat gtgggaagta
     8101 tgagtgtatg aattagtccg gcatgtcatg tttgtctcgc gttgctgcgg ctcatcgagc
     8161 cgcgctccgt ttgatccggt gaacggcacg ggcgattgga actcaggcgg cgcggagggc
     8221 tattgaaaaa tccggcatgt taccttcgtg caacgcccgg ctcaatttgc gaaaagccgc
     8281 taggagggtt tcagacgaag aaaatttaac tagcacgcca ttcgatccga aatcaacggc
     8341 tgacccgaat tgccgatgca atccttcgcc gcgcaagtcg gcaatgacca gatgcctctg
     8401 ctgcggcatg gtcaaccaag gccccgtttc aggcgcgtaa tgattgggga gcgtgacgtc
     8461 ccacagtgca agtaccggca atgggttttc ttggcaatcg gcatttgtgc cgacttcttc
     8521 gagataacgc gttgcgctgt ccgcatctgg caggacgatg agatgattat ggataccggc
     8581 ttgttcaagc agcaggctca acggtggtga tgccgaaagc aaacgttcaa tcaccaaaat
     8641 cggcgc
//
LOCUS       DASZMM010000248         5943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1482241, whole genome shotgun sequence.
ACCESSION   DASZMM010000248 DASZMM010000000
VERSION     DASZMM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482241"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VG754_05960"
     CDS             complement(<1..311)
                     /locus_tag="VG754_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWF18791.1"
                     /translation="MFTGTYTAIVTPFRNGKIDEPALERLIKAQIKGGVDGIVPVGTT
                     GESPTLNYEEHIHVIALSVKFAAGKIKVLAGTGGNSTTEAIYLTQHAEKIGADGSLQV
                     AP"
     gene            complement(404..1213)
                     /gene="dapF"
                     /locus_tag="VG754_05965"
     CDS             complement(404..1213)
                     /gene="dapF"
                     /locus_tag="VG754_05965"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWF18792.1"
                     /translation="MLLGFTKMNGAGNDFVLLDNRAQKIRLTPTQVVHICHRQRGVGA
                     DGVILLIPCASGKADWAWQFFNSDGSVAEMCGNGARCFARFVKKLTGAKDNLTFETGA
                     GIISAEFHGDRVTVNLTQPKELQLEQKINLSTGTQPVHSLNTGVPHAVLFVPDADQAM
                     VQQLGPEIRRHSHFAPKGTNVNFVQRLGPGSIRVRTYERGVEGETLACGTGVTASALI
                     SARLHQFSSPVKVQVQSGDELEVSFQSSKEEFTDVRLTGPADFVFEGKIEV"
     gene            1364..3418
                     /gene="shc"
                     /locus_tag="VG754_05970"
     CDS             1364..3418
                     /gene="shc"
                     /locus_tag="VG754_05970"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWF18793.1"
                     /translation="MSGTSTQDRQLDNKPLQQDFGFRHRADLETAIHRSQNFLLREQR
                     PEGYWVGELMVDSTIVSDTIAYHHWNGKVDKQWQRKAVNHIFSMQLPDGGWNIYHGGP
                     SEVNATIKAYLALKLAGVPATDHRMLRARAVALSLGGVPRMNTFSKLYLALLGLYSWD
                     YVPTIPCEVILLGKWFHVNFYEMSSWSRSMLVPLAIINHFKPTRPLQSPVTLDELYPE
                     GYHERDLALRPDPKLITWRNFFLWLDKLHKFAELWVRAGIHPFRKRALKKCEEWMVER
                     FEGSNGLAAIFPAMLNSLIALKALGYPDDHPEVVRAEHELKNLEHETADSVRIEPCFS
                     PVWDSAIVAICLHESGLPSDHPALKRACEWLMTKEIRFRGDWYYKNPVDVEPSGWVFE
                     FENKWNPDVDDTAMVLLALRKIPTDNPQKRDECFQRGLKWMLTFQCKDGGWAAFDKDC
                     TKGILEKVPFADHNAMLDPECADITARILELMGYEGFGIDHPQIKKAIQFIRDEQEED
                     GSWYGRWGVNYIYGTWQVLRGMRALGMNMNQAWLLKARDWLESVQREDGGWGERCITY
                     DDPVFKGQGPVSTASQTAWAIMALCAFDDPHRPSIVRGVEFLMQTQNPDGTWAEHETT
                     GTGFPRVFYLKYDMYRNSWPLLALANYRNLLAQFPAKEVIRHVNGFKHPVPDALKVPE
                     LA"
     gene            3498..4619
                     /locus_tag="VG754_05975"
     CDS             3498..4619
                     /locus_tag="VG754_05975"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HWF18794.1"
                     /translation="MSWCYRNLLRPVLFAQDSEKIHDRTLRMLAFASRHELICKTLGA
                     FFHSAELPVRAFGLNFPNPVGLAAGMDKQAAAVPVWAALGFGFSELGGVTWHAQSGNP
                     APRMFRAVPDLALINRMGFNNPGAQAMAGTLAEWRKNGRWPTHPVGINLGKSKVTPLS
                     EAAQDYAESLRALWPLADFFVVNVSSPNTPGLRNLQDRAALDEILAALQQTNFALAQA
                     ASSSSPKPILVKVAPDLAFEALDEILELVAPRQVAGIVATNTTITRPETSRPELQGIY
                     SEAGGLSGKPLAIRSTDVIRHIYKQTRGKLPIIGVGGIFDADDAWEKITAGACLIQVY
                     TGLVYEGPGIARNIVSGLRARLAENGLAELAKAVGCRAD"
     gene            4723..5067
                     /locus_tag="VG754_05980"
     CDS             4723..5067
                     /locus_tag="VG754_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWF18795.1"
                     /translation="MTTMTKRDLVIRISNETGLVQQQVLDVVQKTLDYIADAVSKGDK
                     VELRNFGVFEVKVRKARIGRNPNAPATDVPIPQRAVVKFKPGKEMRAEVLKLTPAQLQ
                     ARPSDGSANAAA"
     gene            5198..>5943
                     /locus_tag="VG754_05985"
     CDS             5198..>5943
                     /locus_tag="VG754_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWF18796.1"
                     /translation="MDRLPGFRDFYPEPLPHPDVWSAEARQYIFEKWRGVARRYGFRE
                     YDGPPLEPLELYTTKSGDEIVAQLYNFTDKGQRQIALRPEMTPTLARMVAAHERNYKK
                     PIKWFAIPQLFRYERQQKGRLREHFQFNADIFGESDVAADAELISLLIDTLRSFGLTE
                     KDFVIRLSSRNAWQDYFNRRCPDASKAYEFYQIIDKLEREAPEESKKKLAALGFSFDE
                     ITAFIKTGKPTAELEAILNNLAARGLSDFV"
BASE COUNT         1322 a         1621 c         1683 g         1317 t
ORIGIN      
        1 ggagcgacct gcaacgaacc atcggcgcca attttttccg cgtgttgcgt caggtagatg
       61 gcctcggtgg tggaattgcc gcccgtgccg gcaagcacct tgatttttcc agcggcgaat
      121 ttgaccgaca gcgcgatgac gtggatgtgt tcctcgtaat tcaacgtggg cgattcgccc
      181 gtcgtgccaa caggcacgat gccgtccacg ccgcccttga tttgcgcctt gatcaagcgt
      241 tcgagcgcag gctcgtcaat tttcccgttc cgaaatggcg tgacaatcgc agtgtaagtt
      301 ccagtaaaca tgttcaataa attaaacgag cgtgtaagat agtggaaacg gcccgtttga
      361 ccagcccgtt ttttgcagga gaaaagcccc gaagaattgc cggctagacc tcgatctttc
      421 cttcaaaaac aaaatccgca ggacccgtca ggcgcacatc ggtgaattct tccttgctgg
      481 attgaaagct cacttccaac tcgtcgccgc tttgcacctg gactttcacc ggcgaactga
      541 attggtgcaa tcgggctgaa atcaaagcgg aagccgtcac gccggtgccg cacgcgagcg
      601 tctcgccttc aacgccgcgt tcataagtcc gcacccggat gctgcccggg cccaggcgct
      661 ggacaaaatt cacgttcgtt cccttcgggg cgaaatgcga atgacgccgt atctccgggc
      721 cgagctgttg caccatcgct tggtcggcgt ccggcacaaa aaggactgcg tgcggcacgc
      781 cggtattcaa cgaatggacc ggttgcgtgc cggttgaaag attaattttt tgttcaagct
      841 gcaattcttt tggttgcgtc agattcacgg tgacacggtc accgtgaaat tccgcgctga
      901 tgatgcccgc gccggtttca aatgtcagat tatcttttgc accggttaat tttttcacaa
      961 atcgcgcaaa gcagcgggcg ccgttgccgc acatttcggc gacgctgcca tcgctgttga
     1021 aaaattgcca agcccagtcc gcctttcccg acgcgcatgg aattaacagg atgacgccgt
     1081 ccgcgcccac gccgcgttga cggtggcaaa tatgcacaac ctgggtgggt gttaacctga
     1141 ttttttgcgc gcggttgtcc agcaggacaa agtcattgcc cgcgccgttc atcttggtga
     1201 agcccaaaag catcctttaa tttaacagtt cgcagttgaa agttcaaagt tcaaagttat
     1261 cttaactgcc taggagtggc aggctggctg tgtgacggcc ggcttcaata aaccggtgat
     1321 aaaaaacttt acagtccgtt ggcctgttcg cagagtatcc agcgtgtcag gcacgtcaac
     1381 tcaggacagg caactggata acaaaccgtt acaacaagac tttgggttcc gccacagggc
     1441 ggacctggaa accgccattc atcggtcgca gaattttctg ctgcgcgaac aaaggcccga
     1501 aggttactgg gtcggagaac tgatggtgga ttccaccatc gtctcggaca ccatcgctta
     1561 tcatcactgg aacgggaagg tggacaagca atggcagcgc aaggccgtca atcacatctt
     1621 ctcgatgcaa ctgcccgacg gcggctggaa catttatcac ggcggcccgt cggaagtgaa
     1681 cgcaaccatc aaggcttatc ttgcgttgaa gctcgcgggc gtgccggcca ccgatcaccg
     1741 catgttgcgc gcgcgcgcgg tcgcgttgag ccttggcggc gtgccgcgca tgaacacgtt
     1801 ttccaaattg tatcttgcgc tgctgggatt gtactcgtgg gattatgtcc caacgattcc
     1861 gtgcgaggtc attctgcttg gcaaatggtt ccacgtaaat ttttacgaga tgagttcgtg
     1921 gagccgttcc atgctcgtgc cgctggccat catcaaccat ttcaagccga cgcgtccgct
     1981 gcaatccccc gtcacactcg acgaattgta tcccgaaggt tatcacgaac gcgacctcgc
     2041 cctgcggccc gatccaaagt tgatcacctg gcgcaatttt ttcctgtggc tcgacaagct
     2101 gcacaagttc gccgaactct gggtccgcgc cggcattcat ccgttccgca aacgcgcgtt
     2161 gaagaaatgc gaggaatgga tggtggagcg ttttgaaggt tcgaatggat tggcggcgat
     2221 ttttccggcc atgctcaatt ccctcatcgc gctcaaggcg ctcggttatc ctgatgacca
     2281 tccggaagtc gtgcgcgctg aacacgaatt aaaaaatctc gaacacgaaa ccgcggacag
     2341 cgtccgcatc gagccgtgct tttcgccggt ttgggactcg gccatcgtgg cgatctgtct
     2401 gcatgaatcc ggtttgccga gcgaccatcc cgcgttgaag cgcgcttgcg aatggctgat
     2461 gacaaaggaa atccgttttc gcggcgattg gtattacaaa aatcccgtgg acgtcgagcc
     2521 gagcggctgg gtgtttgaat ttgaaaataa atggaacccg gacgtggacg acaccgcgat
     2581 ggtactgctc gcgttgcgca aaatcccgac ggacaatccg cagaaacgcg acgaatgttt
     2641 ccaacgcggt ttgaaatgga tgctcacgtt ccaatgcaag gacggtggtt gggcggcgtt
     2701 cgacaaggat tgcaccaagg gcattttgga aaaagttcct ttcgccgacc acaacgcgat
     2761 gcttgacccc gagtgcgctg acatcacggc gcgcatcctt gaattgatgg gttacgaagg
     2821 ttttggcatc gaccatccgc agatcaagaa ggccatccag ttcatccgtg acgagcagga
     2881 ggaagatggc tcgtggtatg gacgctgggg cgtcaattac atttacggca cgtggcaggt
     2941 gttgcgcggc atgagggcgc tggggatgaa catgaaccag gcgtggctgt tgaaagcgcg
     3001 cgattggctg gaaagcgtgc agcgcgaaga cggcggctgg ggcgaacgct gcatcactta
     3061 tgacgatccg gtcttcaaag gccagggacc ggtcagcacc gcgtcacaaa cggcgtgggc
     3121 catcatggcg ttgtgcgcgt tcgatgaccc gcatcgtccc agcatcgtgc gcggcgtcga
     3181 atttctgatg cagacgcaga atccggacgg aacatgggcc gagcatgaga ccactggcac
     3241 cgggttccca cgcgtctttt atttgaaata cgacatgtat cgcaacagtt ggccgctgct
     3301 ggcgctggcg aactatcgca atctgttggc tcaatttcca gcgaaggaag tcatccgcca
     3361 tgtcaacggt ttcaagcatc cggttcccga cgcgctgaaa gtccctgagc tggcgtgagt
     3421 gttcagaggt cgaggtttaa agttgatgga cagtcagtgc tcgccttgcg aatggtccgt
     3481 cggctaattt ctccgcgatg agctggtgct atcgcaattt gcttcgcccg gtgttgttcg
     3541 cccaggattc agagaaaatc catgaccgga cattgcgtat gctggcgttt gcgagccggc
     3601 acgaactcat ttgcaaaacg cttggagcat tctttcattc tgcggaattg ccggtcaggg
     3661 cgtttggttt gaattttcca aatccggtgg gtctcgccgc aggaatggac aaacaagccg
     3721 ccgcagttcc cgtctgggcg gcgcttggtt ttggtttcag cgaattgggt ggtgtgacat
     3781 ggcacgcgca atcgggcaat cccgcgccgc gcatgtttcg cgccgtgccc gacctcgcgc
     3841 tcatcaatcg catgggattc aacaatcccg gcgcgcaagc catggctggc acgcttgctg
     3901 aatggcgcaa aaacggacgc tggccaactc acccggtcgg catcaacctt ggcaaatcga
     3961 aagtcacgcc gctgagtgaa gccgcgcagg attatgctga gtcgttacgg gcattgtggc
     4021 cgctggcgga tttttttgtg gtcaatgtca gttcgcccaa cacgccaggc ttgcgcaatt
     4081 tgcaagaccg cgcggcgttg gacgaaatcc tcgcggcctt gcagcaaact aatttcgcgc
     4141 tcgcgcaagc ggcgagttcg tcttcgccaa aaccaatctt ggtgaaggtt gcgccggacc
     4201 ttgcgtttga agcattggat gaaattttgg aattggtcgc gccgcggcaa gttgccggga
     4261 ttgtggcgac gaacacgacc atcacacggc cggaaacttc gcgccctgaa ttgcaaggga
     4321 tctattccga ggcgggcggc ttgagtggca aacctcttgc aattcgcagt accgacgtga
     4381 ttcgccacat ttacaagcaa acgcgtggca aattgcctat catcggcgtt ggaggcattt
     4441 tcgacgcaga cgatgcgtgg gaaaaaatca ccgcaggcgc ttgtctgata caggtttaca
     4501 caggacttgt ctatgaaggt ccgggaatcg cgcgcaatat cgtaagtgga ttgcgggcgc
     4561 gcttggctga aaatgggttg gcagaattgg cgaaggcggt gggttgccgg gcggattgaa
     4621 ccctaagttt ctgcatatta tatacttgac cagtggattt gcggggctta taccggggca
     4681 cggaaacaat aataaggtaa acgctcaacc gccctaacca acatgacgac tatgacaaag
     4741 cgtgacttgg tcatccgtat cagcaacgaa accggcttgg tgcagcagca ggtcctcgat
     4801 gtcgtgcaga agactttaga ttacatcgct gacgcagttt ccaagggaga caaggtggaa
     4861 ttgcgcaact tcggtgtctt cgaagtcaag gtccgcaagg cgagaatcgg ccgcaatccc
     4921 aacgccccgg cgacggacgt gcccattcct caacgcgcag tggtgaagtt caagcccggc
     4981 aaggaaatgc gtgccgaggt gctcaagctc acaccagcgc aattgcaagc ccggccaagt
     5041 gatggcagcg ccaacgccgc ggcttaaagc caattttctt cttgcagtca gccctcgatg
     5101 ttccgcgttg ggcgtccaga agccgaacat caaagaaggt ttgtcaaatt gagggttgct
     5161 tcccttcccc ctttcaaggt tagcttttcc ggctgatatg gatcgtttac caggatttcg
     5221 tgacttttac cctgaaccgc tcccgcatcc ggatgtctgg agcgcggaag cgcggcaata
     5281 cattttcgaa aaatggcgcg gcgtcgcccg ccgttacggc tttcgcgaat acgatggccc
     5341 gccgttggag ccgttggaac tttacacgac gaaaagcggc gatgaaattg tcgcgcaact
     5401 gtacaatttc accgacaaag gccagcgcca aatcgccttg cgacccgaga tgaccccgac
     5461 gcttgcgcga atggtcgcgg cccacgagcg caattacaaa aagccaatca aatggttcgc
     5521 cattccgcaa ctcttccgtt acgaacgcca gcagaagggc cggttgcgcg aacactttca
     5581 attcaacgcg gacatctttg gcgaaagcga tgttgccgcg gacgcggagt tgatttcgct
     5641 tttgattgac acgctgcgct cgttcggttt gacggagaaa gacttcgtca tccggctcag
     5701 cagccgcaac gcgtggcagg attatttcaa ccggcgttgt cccgatgctt cgaaggcgta
     5761 tgagttctat caaatcattg acaagctcga acgcgaagcg cccgaggaaa gcaaaaagaa
     5821 gctcgccgcg ctcggctttt cattcgatga aatcaccgcg ttcatcaaaa ctggaaaacc
     5881 gaccgcggaa ctggaagcca tcctgaacaa tctcgccgct cgcggcctca gtgatttcgt
     5941 gaa
//
LOCUS       DASZMM010000249         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2851042, whole genome shotgun sequence.
ACCESSION   DASZMM010000249 DASZMM010000000
VERSION     DASZMM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2851042"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..163
                     /locus_tag="VG754_05990"
     CDS             5..163
                     /locus_tag="VG754_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWF18797.1"
                     /translation="MQRRKIENILKTGAQIVVTTNPGCLLQIRAGLAKVGSKIEALHI
                     ADFLDRVK"
     gene            209..1429
                     /locus_tag="VG754_05995"
     CDS             209..1429
                     /locus_tag="VG754_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF18798.1"
                     /translation="MGSTETRKERHSSATWLNRTVLGIGLASLFSDWSHETATTVMPA
                     FLATMGAAALWLGLIEGVSDGLSSFAKMGSGYYTDRLPRRKPIAVIGYIVTTLGTAAI
                     GLATAAWHILIARAFAWLGRGVRTPVRKALLAGSVTKETYGRAFGFERMMDTVGAIVG
                     PATAVILLQVFHHHYRMLFAATLAPGLVAAGLILFLVKERERKPVPHISFGENLKLLP
                     RTYRKFVLAAGLFGVGAFAHTMLILLATQRLSPSMGAARAASVAVGLYILHNIFYASF
                     AFIGGWLADRIQKNYLLAAGYSMAAAMSLCIIFLPANVGTLALIFILGGTNVALEETL
                     EDSFCAELVDEPHHGMAFGVLATVNGVGDFASSIIVGALWNTVGTTAAFGYSAALSLL
                     GAFLVLRIRPGTDH"
     gene            complement(1447..2133)
                     /locus_tag="VG754_06000"
     CDS             complement(1447..2133)
                     /locus_tag="VG754_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18799.1"
                     /translation="MASSMFMFRHDAKIFVKRPRRAFTLIELLVVIAIIAVLAGLLLP
                     ALARAKERGRQTSCINSVRQQSLAVLMYADDHGSILPPTAYEDALRNEIDWPALLDPY
                     LNYSAKIHLCPSDQKSQTNSYGLNELVFVDLTDDNPPPPLNVSAFLSPSASIMQGDIG
                     TEDDFKTPRPDTLKLTAPGSPLNDDEDARPSSRHLNRCDLGFMDGHSEHLMLGQFYTN
                     QTPVNKWFTP"
     gene            complement(2164..3057)
                     /locus_tag="VG754_06005"
     CDS             complement(2164..3057)
                     /locus_tag="VG754_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18800.1"
                     /translation="MSRNLSRACRPVASAFTLIELLIVIAIITILAGLLLPALARSKG
                     RAYGVQCMNNQRQLIFAWQLYYTDFDDRLVYNLGGNVSNNRASVAPSGQPNWVDNVMD
                     WTLSPDNTNTAFATTSLLGPYASHSIPIYKCPADHALSDVQRAAGWNARVRSVSMNAM
                     VGDPGNLLTGGANINNPDYRQFLKQGDIPKSSDIFVFLDEHPDSINDGYFIDKPAYPS
                     TSSYGYTPASFEWLDLPGSYHNGAGSFSFADGHAEIHPWRYDNTKPPSVAEGATLPMW
                     INANQTDDIYWVLKHMSILSN"
     gene            complement(3334..3786)
                     /locus_tag="VG754_06010"
     CDS             complement(3334..3786)
                     /locus_tag="VG754_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18801.1"
                     /translation="MSVIKVSLVLVAEDDENDIFFLERAFKQAQVVNPIFRVRDGEDA
                     IAYLRGDDAYADREKHPLPQLMLLDLKMPRKNGFEVISWVRDQPGLKRLPIVVLTSSK
                     EDPDINRSYELGANTYLVKPVKFEGLVDMMRALNLYWLLLAEKPNCVP"
BASE COUNT          855 a         1065 c         1126 g          929 t
ORIGIN      
        1 acgcctgcaa cgccgcaaga ttgaaaacat tctcaagacc ggcgcgcaaa tcgtggtcac
       61 caccaatccc ggttgccttc tgcaaatccg cgcagggttg gcaaaggttg ggtcaaagat
      121 cgaggcgctc cacattgcgg attttttgga tcgggtgaaa tgagcagcag agcgcagaaa
      181 ctttaaacca gcacaccagt cactcccaat gggctcaacg gaaactcgca aagagcgtca
      241 ttcttctgcc acctggctca accgcaccgt tttgggcatc ggtctggcct cgctgttcag
      301 cgattggtcg catgaaactg ccaccacagt catgcctgca ttcctggcca ccatgggtgc
      361 cgctgcgctc tggctcggct tgattgaagg tgtgtcggat ggtctttcga gttttgccaa
      421 gatgggttcc ggctactaca ccgaccgcct gccccgtcgc aaacccattg cggttatcgg
      481 ctacattgtt accaccctcg gaacggctgc cattggcctg gcaactgcgg cttggcacat
      541 cctgatcgcg cgagcgtttg cctggctcgg gcggggcgtg cggacaccgg ttcgcaaggc
      601 gctgctggcc ggttcagtga caaaagaaac ttatggccgc gccttcggtt ttgagcggat
      661 gatggatacg gtgggtgcca tcgttggccc ggcaaccgcc gtcatcctgc tacaggtgtt
      721 ccatcatcac tatcggatgc tctttgcggc aactctggcg ccgggacttg ttgcagccgg
      781 tctgattctg tttctcgtga aagaaagaga acgcaaaccg gtgccgcaca tttcctttgg
      841 ggaaaatttg aaattactgc ctcggactta ccgcaagttt gtcctggcgg ccgggttgtt
      901 tggcgtcggc gctttcgcgc acacaatgct catcttgctg gccacccaga gattaagtcc
      961 ttccatgggt gcggcaaggg ccgcaagtgt cgctgtcggg ctctacatcc tgcacaatat
     1021 tttttatgct tcgttcgcat tcatcggcgg ctggcttgcg gacaggatcc aaaaaaacta
     1081 tctcctggct gcgggctatt cgatggcggc ggccatgtcc ctgtgcatca tttttctgcc
     1141 tgccaatgtt gggacgctgg cattgatctt tattctcgga ggaacgaacg tcgcgctgga
     1201 ggaaactttg gaagattcct tctgcgcgga gttggtggat gaaccgcatc acggcatggc
     1261 cttcggcgtc ctcgccacag tcaacggagt tggggatttt gcatcgagca tcatcgtcgg
     1321 cgcgctttgg aacacggtgg gcacaaccgc agctttcgga tacagcgcgg cgctctcgct
     1381 gcttggcgct tttctggtgt tgaggattcg cccgggaaca gaccactaac ctctgactcg
     1441 ccgtcatcag ggcgtgaacc atttgttcac gggcgtttgg ttcgtgtaga actggcccaa
     1501 catcaggtgc tcgctgtgcc catccatgaa accaaggtcg caacggttca gatggcgcga
     1561 tgaggggcgg gcatcctcgt catcgttcaa aggcgacccg ggcgcggtga gtttcaaggt
     1621 gtcggggcgc ggcgttttga aatcgtcctc agtcccgatg tcgccttgca tgatgcttgc
     1681 gctgggagaa agaaacgcgg acacattcaa aggcggaggc gggttgtcat cagtcaaatc
     1741 aacaaacacc agttcgttga ggccgtaaga attggtctgc gatttctggt cggacgggca
     1801 aagatgaatt ttcgcggaat aattcaggta aggatccagc aacgccggcc agtcaatctc
     1861 gtttctcaac gcgtcttcat aagcggtcgg cggcaggatg ctgccatggt cgtcggcgta
     1921 catcagcacg gcgaggctct gttgccgcac gctgttgatg caggaagttt ggcgtccccg
     1981 ttcctttgcc cgcgccaatg ccggcaacaa aagcccggca agcaccgcga tgattgcgat
     2041 gacgacgagc aattcaatca acgtgaatgc ccggcgcggg cgcttgacaa aaatttttgc
     2101 atcatggcgg aacatgaaca ttgaggatgc cacgttaaaa gaagtttgcg agcttacttt
     2161 agtttaattg gacaaaatgc tcatgtgctt gagcacccaa tagatgtcgt ccgtctgatt
     2221 ggcgttgatc cacatcggga gcgtcgcgcc ttcggccacg gaaggaggct tggtgttgtc
     2281 ataacgccat ggatgaatct ccgcatggcc gtccgcaaaa gaaaaacttc ccgcgccatt
     2341 gtgatacgag cccggcaaat caagccattc gaaagaagca ggcgtgtaac cataactgct
     2401 tgtgcttggg taagccggtt tgtctatgaa ataaccgtca ttgatgctgt cgggatgttc
     2461 atcaaggaag acgaagatgt ccgacgattt gggaatgtct ccctgcttca agaattggcg
     2521 ataatctgga ttatttatgt tcgcgccgcc ggtcagcagg ttgccggggt cgccgaccat
     2581 cgcattcatt gaaacgctgc ggacgcgcgc gttccagccc gcggcgcgtt gaacatcact
     2641 gagcgcatgg tccgcggggc atttgtaaat cgggatggag tggctggcat acggcccgag
     2701 cagcgaggtg gtggcgaacg cggtgttggt gttgtccggg ctgagcgtcc aatccatcac
     2761 attgtccacc caattcggct ggcccgaagg cgccacgctc gcgcgattgt tgctgacatt
     2821 tccgcccagg ttgtaaacca gccggtcatc gaaatccgtg taataaagct gccacgcaaa
     2881 aatgagctgg cgctgattgt tcatgcattg aacaccgtaa gcgcgtccct tggaacgggc
     2941 cagggccggc agcaacaaac cggcaagaat cgtgatgatg gcaatgacaa tgagcaattc
     3001 gatgagcgtg aatgcagaag caaccggacg gcacgctcgg gaaagattgc gggacacctt
     3061 tgccggaagg caccggctta atattcgttg ctggagttca agtgtcactg gaccagcgca
     3121 aaggctgttc tcaatagtgg catgatacta acgcaggaga gctggtgtgt aaagaggaat
     3181 tggggtgaag ctccaaccgg cattgattgt attgtcagta cggctaaaga atgatttggg
     3241 tgaacgcctt cgcttgcgat ggctttgatt cggaacgact gtggagtgtt tgttaaaaca
     3301 cctgttccct gttgcccgtt cttcaggaat agatcaaggc acgcaattcg gtttttcagc
     3361 cagcaacagc caatataaat tgagcgcgcg catcatgtcc accaagccct cgaacttgac
     3421 cggtttgacg agatacgtat tcgccccaag ttcgtaagag cggttgatgt ccgggtcttc
     3481 tttcgaggag gtcaggacga cgatgggcaa ccgcttcaac ccgggttggt cgcgcaccca
     3541 tgaaatgact tcgaagccgt tttttcgcgg catcttcaaa tccagcagca tcaattgcgg
     3601 caagggatgt ttttcacggt cggcgtaagc gtcatccccg cgcagataag cgatggcgtc
     3661 ctccccgtcg cggacgcgaa agatcgggtt gacgacctgg gcctgcttga atgcacgttc
     3721 gaggaagaag atgtcgttct cgtcgtcttc agcgaccaaa accaagctga ctttgataac
     3781 gctcatacta taagttgtat tgccgcgtgc aaccaacgca atccaattct ttcgaatgat
     3841 gccctttccc atacagcatc atcatgtatc ttgacatgcg ttttcaaatc acccggacaa
     3901 gccctcactt cttccgcgat gccagcttct ccaccctgcg catcgctgat ttcccgtgcg
     3961 cgtccagtcc ttcga
//
LOCUS       DASZMM010000250         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2297022, whole genome shotgun sequence.
ACCESSION   DASZMM010000250 DASZMM010000000
VERSION     DASZMM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297022"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..900
                     /locus_tag="VG754_06015"
     CDS             367..900
                     /locus_tag="VG754_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWF18802.1"
                     /translation="MTPLAPVQQKFILHWGEMGTRWGINRTVAQIHALLYISPKPLHA
                     EEIVESLGVARSNVSTSLKELQGWGIVKMVHVLGDKRDHFESMKDVWELFRVVLDERK
                     RREIDPTLVMLRECITEAEQEKAKDSYTLERLQALRDFFELTTSWYMQVRNWPASTLA
                     KFVKLGDKALKLLSLTR"
     gene            923..1300
                     /locus_tag="VG754_06020"
     CDS             923..1300
                     /locus_tag="VG754_06020"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HWF18803.1"
                     /translation="MNTEITERIKEWLFYDAECAICRGWVQRLRKRLHRQGIGVAPLQ
                     AEWVGERLGLAPGELPEEMKLLTSTGEIFGGADVIIYLARYFWWGWGLRVVAHLPGAR
                     FVLRRAYAWLARHRYCLKGGCKV"
     gene            1297..2232
                     /locus_tag="VG754_06025"
     CDS             1297..2232
                     /locus_tag="VG754_06025"
                     /inference="COORDINATES: protein motif:HMM:NF025185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HWF18804.1"
                     /translation="MSRITKDLVGFFPTDIQQKGLFRTQGWRNLPGWLPLLILPCIAW
                     IFRNSIAPWIFMWAMAFALYFGCKWLTWWRCAVPGVPLWRHLAYLFAWLGMDANAFLD
                     PRKSVPKPQLREWIAAILKTVFGALLVWWIVKLIGQPLLAGWIGLVGCIFLLHFGTFH
                     LLALLWQQLGIDAQPIMRAPILAKSLADFWGTRWNSAFNLLVHKLVFRKIYRHIGATG
                     ATLAVFLVSGIIHDLLISVPARAGYGLPTIYFLLQGVGLCFERRKLNQAPLLHHEIAR
                     RSFTIAVTAIPAFWLFHPPFIRNVILPMLHAIGAT"
     gene            2240..2659
                     /locus_tag="VG754_06030"
     CDS             2240..2659
                     /locus_tag="VG754_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18805.1"
                     /translation="MKTETVTLLLQIAGVLHLGLMAAGLSMPRVVNMRVHLAKLPPFL
                     QRLFWVYYTFIAFCLVSFGLVSFFFAGTLAAGTGLARAACAFMAIFWTIRLIAALFVF
                     DVRPYLTNAYWKLGYAATNLVFACFPIIYAIAAWKRN"
     gene            2660..>3268
                     /locus_tag="VG754_06035"
     CDS             2660..>3268
                     /locus_tag="VG754_06035"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HWF18806.1"
                     /translation="MNAIACQRECLSEAARRRILSAKGEPRFYADWLRAVFIHYEVDA
                     EYLQRAVPFELDLDEGRAYVSLVAFTMRGMRPRIGGKVGALLLKPIAGHGFLNVRAYV
                     RHRGETGIYFLAEWLSNPVSVMLGPRCFGLPYRLGHLEYEHRHETGLLRGNVIDGKNG
                     ADGTNIAPRLEYSAKINPVSAFEPCAAGSCAEFLMERYTAFTE"
BASE COUNT          697 a          822 c          930 g          819 t
ORIGIN      
        1 ctgtatctgg tcccaaatgg cctgccattt gggttcgatc aaatcaaacg gatactgttt
       61 gcggccactg gacataaata agcgtgcgat attggagaaa cggacgattc tgggcaatgg
      121 cagaattcaa tcctgttcaa attgccagag gagcttgccg gggtcaaatg gcagcggagt
      181 taaaagccac ggatgaaaca cagattaaac gcagacgaaa acagcgtaca tacgcgcaag
      241 gattaacgtc caataagctc ccattggctc ctttagcgtt tcatccgtgt tcactcggtg
      301 gccaaaatac ttgttgacat ttaatgttat tatggtaatt tctgaataga cagaaattaa
      361 agaattatga cgccactggc accagttcag cagaagttca tcctccattg gggagagatg
      421 ggcacgcgct ggggcatcaa ccggacggtg gcgcaaatcc acgcgctcct ttacatctcg
      481 cccaagccgc tgcatgcgga agaaatcgtc gaaagcctcg gcgtggcgcg gtcgaacgtc
      541 agcaccagcc tcaaggaatt gcaggggtgg gggatagtca aaatggtcca tgtccttggt
      601 gacaagcggg accactttga atccatgaag gacgtttggg aattgttccg cgtggttttg
      661 gacgagcgca aacgccgcga aatagatccc acgctggtga tgcttcgcga gtgcatcacg
      721 gaagcagagc aagaaaaagc gaaagattct tacacgttgg aacggctgca agcgctcagg
      781 gactttttcg agctgaccac gtcctggtac atgcaggtgc gcaactggcc ggcaagcacg
      841 ctggcaaagt ttgtgaaatt gggagacaaa gccctgaaac tgttgagcct cactcgataa
      901 atttttttgc ggagctattt ctgtgaatac agaaattaca gaaagaatca aagaatggct
      961 gttctacgac gccgaatgcg ccatttgccg tggttgggtc caacgattgc gcaaacggtt
     1021 gcatcgccag ggaatcggtg tggcgccgtt gcaggcggaa tgggtgggag agcggctggg
     1081 acttgcgccg ggagaattgc ccgaagaaat gaaacttctt acgagcactg gagaaatctt
     1141 cggcggcgcg gacgtcatca tttatctggc ccgatatttt tggtggggat ggggactgcg
     1201 cgtcgtggcg catctacccg gcgcgagatt cgtcttgcgc cgggcttacg cctggcttgc
     1261 acggcatcgg tattgcctga aaggaggatg caaagtatga gccgcatcac gaaagacctc
     1321 gtcggctttt tccccaccga catacagcag aagggcctgt ttcggacgca aggctggcga
     1381 aatttgccgg gatggttgcc gcttctcatt ttgccttgca tagcttggat tttcaggaac
     1441 tcaatcgccc cttggatttt catgtgggca atggcattcg cgctttattt tggctgcaaa
     1501 tggctgactt ggtggcgatg tgctgttccc ggagtccctt tgtggcgaca tttagcttat
     1561 ctattcgcat ggctgggaat ggatgcgaat gcttttcttg acccgcgcaa gtctgttccc
     1621 aagccgcaac tgcgcgaatg gattgccgcc attctcaaga ccgtcttcgg agcattgctg
     1681 gtttggtgga tcgtgaaact catcggccag ccgttgttgg caggttggat cggcctcgtt
     1741 ggttgcattt ttcttctcca tttcgggaca tttcatcttc tggcgctcct gtggcaacag
     1801 ctcggtatcg atgcccaacc catcatgcgc gcgcccattt tggcgaaatc gctcgcggac
     1861 ttttggggaa cgcgctggaa ttcggctttt aatctcctgg tgcacaaatt ggtttttcga
     1921 aaaatctacc ggcacatcgg tgcgactggc gcgacgcttg cggttttcct tgtctcggga
     1981 atcatccacg atttgttgat ctcggtcccg gcccgggcgg gatacgggtt gccaaccatt
     2041 tactttctcc tgcaaggcgt cggcctttgc ttcgaacgac gtaagttgaa tcaggcaccg
     2101 ttgctccatc acgaaatcgc gcgacgaagc ttcaccatag cggtgaccgc gattcccgca
     2161 ttttggctct ttcatccgcc gttcatcaga aacgtaatcc tgccgatgct tcatgccatc
     2221 ggcgcaacct gaatcgccta tgaaaactga aaccgtcact cttctgcttc aaatcgccgg
     2281 tgttctccac ctcggattga tggctgccgg tttgtccatg ccgcgggtgg tgaacatgcg
     2341 cgttcatctc gcaaaactgc cgccattcct gcagcggctc ttttgggttt attacacatt
     2401 catcgcgttt tgtttggtca gtttcggttt ggtaagcttc ttttttgccg gcacgcttgc
     2461 cgcaggaacc ggccttgcga gggcggcctg tgcgttcatg gcaatctttt ggaccatccg
     2521 cctgattgcg gcgctgttcg tctttgatgt gcgtccctat ctcacgaatg cctattggaa
     2581 gctcggttat gccgcgacca atctggtgtt cgcctgcttt ccgatcatct acgcaatcgc
     2641 tgcatggaaa aggaactgaa tgaacgccat cgcctgccag cgagaatgcc tcagcgaagc
     2701 agcgcgacgc cgcatcctga gcgcgaaggg cgagccgcga ttttatgcgg attggctgcg
     2761 ggcggtgttc atccattacg aagtggatgc ggaatatttg caacgagccg tcccattcga
     2821 attggatttg gacgagggca gggcgtatgt gagtctggtc gcgttcacga tgcggggaat
     2881 gcgtccgcga atcggaggaa aagttggagc gttgttgctc aagccgattg cggggcacgg
     2941 tttcctgaac gtgcgggcgt atgtccgaca tcgcggtgaa acgggaattt atttcttggc
     3001 cgaatggctt tccaatccag tgagcgtgat gcttgggccg cgttgttttg gcctgccgta
     3061 ccggcttggg catctcgaat atgaacatcg gcatgagacc gggttgctta ggggaaacgt
     3121 gatcgatgga aagaatgggg cggatggtac caatatagcg cctcgacttg aatactcggc
     3181 caaaatcaat cctgtttcag cttttgagcc gtgcgccgcc ggttcgtgcg cggaattcct
     3241 gatggaacgt tacaccgcct ttaccgaa
//
LOCUS       DASZMM010000251         4735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3118158, whole genome shotgun sequence.
ACCESSION   DASZMM010000251 DASZMM010000000
VERSION     DASZMM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118158"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..747)
                     /locus_tag="VG754_06040"
     CDS             complement(1..747)
                     /locus_tag="VG754_06040"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HWF18807.1"
                     /translation="MNTTPAQVKPWLDTLLGFVYPEVCQICGNEQATASEGFVGARCW
                     RKVRFIKPPFCERCGLPFEGDITTAFECANCRELKLHFRTARSAVIADKRFMLEIIHR
                     YKYQRALWFEPFLADLLIREAAPVLKKEKCDMIVPVPLHPAKRREREFNQAARLARHL
                     SRATGIPVNTDLLERVEPTQTQTRLTRQERAENVQNAFALRKGEKLNGQRVVVLDDVF
                     TTGATTSACAKVLLSSGADDVCVWTVARGL"
     gene            complement(744..1637)
                     /locus_tag="VG754_06045"
     CDS             complement(744..1637)
                     /locus_tag="VG754_06045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWF18808.1"
                     /translation="MRPIGALLIYLVVVFLGGALLAPQLYWLVNGIAPDSHLAHNPFH
                     RFVNRSLLALALLGIWPLLRKLGANSLRDVGLVNPVGQWKLFLAGFAFGFGSLACVAF
                     VTLAAHGRQLNSSLTLGELGGGVAGAIGTALVVATLEEILFRGAIFGGLRRYWDWRAA
                     LIFSSMVYAIVHFMQKADPSGPIVWSSGLKLLPHMLRGFANWQEIIPAFFNLTLVGVL
                     LGLAYQRTGNLYCSIGLHAGWIFWLRAYGMLTVSTPENTNTWFWGSQKLIDGWLTFGV
                     LAVPLVALMFLPMRKTRISPA"
     gene            complement(1767..2006)
                     /locus_tag="VG754_06050"
     CDS             complement(1767..2006)
                     /locus_tag="VG754_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HWF18809.1"
                     /translation="MTIELPDAAVAEGFTPEELRLELACALYARGRIGKIAGAEMAGV
                     DFFTFQRALEERQISIYDEQMLADDLQTLDKLFPQ"
     gene            2155..3006
                     /locus_tag="VG754_06055"
     CDS             2155..3006
                     /locus_tag="VG754_06055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWF18810.1"
                     /translation="MFFKSKKANDVSPGPGQFGPYYLQEMINSGGMADIWLATDKDQN
                     TYALRRLHEDLRFDFTSRKRFWRGCEVLSKIHNHDCIVSYYGHGKISGTMYLLMEYVE
                     GSNLKLMYSDHDPVLLENVASIIIDMGTGLEHVHESGFMHLDFKPENVLVTRNASVRL
                     ADFDLSLPIPKSPAKLSKNPGTPAYMAPEQLRGDPVDQRVDIFAFGVSAYELLTNYKP
                     FPGDTVAEILVKQMNRAGFISPRQHNPDIPVNLEKVILKCLQTDPNQRYLFMAVLMHE
                     LKTALYV"
     gene            complement(3016..3474)
                     /locus_tag="VG754_06060"
     CDS             complement(3016..3474)
                     /locus_tag="VG754_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18811.1"
                     /translation="MNENAILLVEDSSQDANLILAAFKKWGVNNPIQVVLDGERAVEY
                     LGGQGEYADRDKHPLPCLALLDLHLPQMSGFEVLQWIRAQPNLTNLPVVVLSGTKDPS
                     HFEEAQRLGANACVPKTLEHTALFELIQHLNYFSVASDYNNADVPWTPEP"
     gene            complement(3617..>4735)
                     /locus_tag="VG754_06065"
     CDS             complement(3617..>4735)
                     /locus_tag="VG754_06065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWF18812.1"
                     /translation="PYLKTMELKVGNLIGQGQISINVAKQAGDERAIAIQQIKPGSGW
                     QLSKDSYDRDAIRGLNLKIAQNKFKAITSVVVIKDGGLLIEEYFNGANRETKHDPRSA
                     TKSFTSALMGIAIGQGHIKDENVTLKSFYDLTPYSNYSAGKDSITLKSLLTMSSPLDG
                     SDMNEDSPGNEEKMYPTSNWVKFALDLPIDATKATNAHWDYFTAGVILLGDILNKSVP
                     RGLEQYSAQKLFGPLGITNFAWQETPQHVVNTAGSLQMNSLDYAKFGQLYKNGGSWNG
                     LQIIPKSWIDSTFTKAFEIPERTNEYYGYLFWEKSYPVRGKSYETWYCSGNGGSSIFV
                     FRDLRLVVVITAAAYNQPYGHSQIDKIMERYILPAVAN"
BASE COUNT         1142 a         1315 c         1176 g         1102 t
ORIGIN      
        1 tcaaagaccc cgggccacgg tccaaacgca aacatcgtcc gcacccgaag acagcaatac
       61 tttggcacaa gcactggtgg ttgcgccagt cgtaaaaaca tcatccaaca ccacgactcg
      121 ctggccattc agcttctcac ccttgcgcaa ggcgaatgca ttttgcacat tttcggcgcg
      181 ttcctggcgc gtaagccgtg tttgcgtttg tgtgggttcg acgcgttcaa gcaagtccgt
      241 gttcaccgga attcccgtcg cgcggctgag atgccgggcc agccgtgcgg cctggttgaa
      301 ttcgcgctcg cgccgttttg cgggatgcaa tggaaccggg acaatcatgt cgcatttttc
      361 tttcttcaac accggcgcgg cttcgcggat gagcaggtcg gccagaaacg gctcgaacca
      421 gagcgcccgt tgatatttgt agcgatggat gatttcgagc atgaaccgct tgtcggcgat
      481 gactgcggaa cgcgcggtgc ggaagtgaag tttgagttcg cggcaattcg cgcattcaaa
      541 cgcggtggtg atgtcgcctt cgaaaggcag tccgcaacgc tcgcagaaag gtggtttgat
      601 gaaacgcacc ttccgccagc agcgcgcacc cacaaagccc tccgatgcag tcgcctgttc
      661 atttccgcat atttggcaaa cttcagggta aacaaaaccg agaagcgtat caagccacgg
      721 tttgacttgg gcgggcgtgg tgttcatgcc ggtgagatgc gtgttttgcg catcggcaaa
      781 aacataagcg caaccaacgg cacggccagc acaccgaacg tcagccagcc atcaatcaac
      841 ttttgcgaac cccagaacca cgtgttcgtg ttctccggcg ttgagacagt cagcattcca
      901 taagcgcgaa gccagaatat ccaaccggcg tgcaggccga tggaacagta aagattcccg
      961 gtgcgttgat acgccagccc gagcaaaacg cccacgagcg tgagattgaa aaaagctgga
     1021 attatttcct gccaattcgc aaatccgcgc agcatgtgcg gcagcagctt caacccggac
     1081 gaccagacaa tcgggccgga cggatcggct ttttgcatga aatgaacgat ggcgtaaacc
     1141 atgctgctga atatcagcgc ggcccgccaa tcccaatatc tccgcaagcc gccgaaaatc
     1201 gcgccgcgaa aaagaatctc ctccaatgtt gcaaccacca atgcagtgcc gatcgctccg
     1261 gcaactccgc cgcccagttc ccccaaggtg agcgacgaat tcaattgccg tccatgcgcg
     1321 gcgagcgtca cgaacgccac gcaagcgagc gagccaaagc caaatgcgaa cccggcgaga
     1381 aacagtttcc attgtccgac aggattcacc agccctacat cgcgcaggga gtttgcgccc
     1441 agcttgcgca gaagcggcca aatcccaagc agcgccagcg cgagcaggga acgattgacg
     1501 aacctgtgga acggattgtg cgccaaatgc gagtccggcg cgatgccgtt gaccagccag
     1561 taaagttgag gcgccagcaa cgcgccgccg agaaaaacaa caaccaaata aatcagcaac
     1621 gcgccgattg ggcgcattcg gttcccgggc tggatggcca tgtcttgcgt aacagtaatg
     1681 cgggcattca atcacgaacc cttgcgaaaa cgccagcaaa cttcgacgag attattcctt
     1741 gagaaaggaa gtgtgggtta cgacgatcac tgcgggaaca gtttatccag cgtttgcaag
     1801 tcgtccgcca gcatttgctc gtcgtaaatt gaaatttgcc gctcttccaa tgcgcgctgg
     1861 aaagtaaaaa aatccactcc ggccatctca gcccccgcaa tcttgccgat gcgtccacgt
     1921 gcatacaatg cgcaggccaa ctccagccga agttcctccg gcgtgaatcc ttccgcaaca
     1981 gccgcatctg gcagttcaat cgtcacatga tttatttact ttgaaatcgg ccactcggca
     2041 agattaaagc cttgggcaca taagccgttg gcattccaat ccaatcattc tgtctcaaaa
     2101 ccaccattta aaacctagtg gcgggaactg ccacggcgtg gtacatagtc gcaggtgttt
     2161 tttaagtcga agaaagcaaa cgatgtttct cccggaccgg ggcagttcgg gccgtattat
     2221 cttcaggaga tgatcaacag cggcggcatg gccgacatct ggcttgccac cgacaaggac
     2281 caaaacactt acgccttgcg ccggctgcat gaagatttgc gcttcgattt cacttcgcga
     2341 aaacggttct ggcgcggttg cgaagtcctt tccaaaatcc acaatcacga ctgcatagtc
     2401 agttattacg ggcacggaaa aatcagcgga acgatgtatt tgttgatgga atacgtggaa
     2461 ggctcgaatc tcaaactgat gtacagcgat cacgatcccg ttctgctcga aaatgtcgca
     2521 agcatcatca tcgatatggg caccgggctg gaacatgtgc acgagagcgg gttcatgcat
     2581 ttggatttca agcccgaaaa tgtcctcgtg acgcgcaacg cgagcgtgcg tctcgccgat
     2641 ttcgacctct cgctgcccat tccaaaatcg cctgcaaaac tttccaaaaa tcccggcaca
     2701 ccggcttaca tggcgcccga acaattgcgc ggtgatccag tggaccaacg cgtggacatc
     2761 tttgcctttg gcgtttcggc ttacgaattg ctgacgaact acaaaccttt ccccggcgac
     2821 accgtcgcgg aaattcttgt gaaacagatg aaccgcgccg gtttcatttc gccgcgccag
     2881 cataatcccg acattcccgt caatctggag aaggtgatct taaagtgtct gcagacagac
     2941 ccgaaccaac gctatctctt catggccgtt ttgatgcacg aactgaaaac ggcgttgtat
     3001 gtataggtga aatgcttacg gttccggcgt ccaaggcacg tcggcgttgt tgtagtccga
     3061 agccaccgag aaataattca aatgctggat gagttcaaag agcgctgtat gctccagcgt
     3121 tttcgggacg caagcattgg ccccgagacg ttgggcttcc tcaaaatggc tggggtcttt
     3181 tgtgccgctc aacacgacca ctgggagatt ggtcaaattc ggttgcgccc gtatccattg
     3241 caacacctcg aagcccgaca tctgcggaag gtgaaggtcc agcaatgcaa ggcagggaag
     3301 gggatgtttg tcgcggtcag cgtactcgcc ctggccaccc agatattcca cggcccgttc
     3361 gccatccaac acgacctgaa tcgggttatt gacaccccat ttcttgaagg ccgccaaaat
     3421 caaattggcg tcctgggagc tgtcctcaac cagcaatatc gcattttcgt tcatgtcagc
     3481 ttcgttccag catcacattg tgacaagact ggagacgtac gaaggccgtg tcaggttaac
     3541 aatttttttt cgtgaagaca cttaattttg cgtcgcgcac ttggccaaga ttgccattgc
     3601 ctcaattcaa cgggttttag tttgccaccg ccggcagaat gtatcgctcc ataatcttgt
     3661 caatttgcga gtgtccgtaa ggttgattgt aggcggccgc ggttatcaca accacgaggc
     3721 ggaggtctct aaatacaaaa attgagctgc ctccgtttcc actgcaatac caagtttcat
     3781 acgactttcc ccttacaggg taagattttt cccaaaacaa atacccataa tattcattcg
     3841 tccgttcggg aatctcaaaa gccttggtaa acgtggaatc gatccaactc tttggaatta
     3901 tttgcaggcc attccaactg ccaccatttt tgtaaagttg cccaaacttt gcgtagtcca
     3961 gcgaattcat ttgcaaactc ccggctgtgt tgactacatg ctggggggtt tcctgccacg
     4021 caaagttcgt tattcctaat gggccaaata gcttttgtgc ggaatattgt tccaagcctc
     4081 ttggaaccga tttatttaag atatcgccta aaaggataac tcccgccgta aaataatccc
     4141 aatgcgcgtt ggttgctttt gtggcatcaa tcggaagatc caacgcgaat ttcacccaat
     4201 tggacgtcgg atacattttt tcctcgttgc ccggggaatc ctcgttcatg tccgagccat
     4261 ccaatggcga actcatcgtc agcaagctct tgagggtaat gctgtccttg cccgctgaat
     4321 agttcgaata cggggtcaag tcatagaagc tttttaaggt cacattttca tcttttatat
     4381 gcccttgtcc tatggcgata cccatcaacg ccgaagtaaa cgacttggtt gcggagcgcg
     4441 gatcgtgctt cgtctccctg ttggctccgt taaaatattc ttcaatcaac aatccgccat
     4501 ccttgatgac aacgacgctg gtgatggcct taaacttatt ctgggcgatt ttcaaattca
     4561 gtcccctgat ggcatccctg tcgtaactgt ctttcgacaa ttgccagccg ctccccggct
     4621 ttatttgctg gatggcgatg gccctctcat cacccgcctg ttttgcgaca ttgatgctga
     4681 tttgtccttg tccgatcaag ttcccgacct ttagttccat cgttttcaag tatgg
//
LOCUS       DASZMM010000252         5076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3946955, whole genome shotgun sequence.
ACCESSION   DASZMM010000252 DASZMM010000000
VERSION     DASZMM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946955"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1639
                     /locus_tag="VG754_06070"
     CDS             197..1639
                     /locus_tag="VG754_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HWF18813.1"
                     /translation="MSGAFAFRLYDEQGFPLDLTELMARERGLTVDKEGFDKLMEEQK
                     ARARAAQKKEIISAGEIDTSSDTNFVGFESLETNATVVAHGLLNGKPYLMLNESPLYA
                     EMGGQVGDTGIFEVDGKRFTILNTIKKGNVWLHFTAETEKPKINTTGVVKVDVKRREA
                     IQRHHTVTHILHWALHEVASREASQKGSFVGPDKLTFDFNSAPLMPAQISDIEKLVNE
                     RIVENSGVSWTEVSYADVKSRKDVMQFFGDKYGDLVRVVQIGGDANKLNGYSMELCGG
                     THTRATGEIGLFRILSESAVAAGVRRIEAVAGLSAYQAARQEAELLRSVAGRVNAPVA
                     ELEKKIEALLAQQKELEKSLRAANQKEAAGRAKELLVKADTSGGVPSIIANIPGADGD
                     TLQTIVDSLKGQFKGVVVLGGAVNGAVGLVASVSPEFTNKLAAGKIIQAIAPIVGGKG
                     GGKPDNARGGGKDVAKLDEALAKAKELSKV"
     gene            1682..1936
                     /locus_tag="VG754_06075"
     CDS             1682..1936
                     /locus_tag="VG754_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18814.1"
                     /translation="MKNLFLCVTLAMFAMASSVLADSNTTAKAAADKSGDCCGSNNCC
                     DSKGHCADKSKSSAKSACSKGMPSKSVLMSPKAAAAAGKS"
     gene            complement(2079..3854)
                     /locus_tag="VG754_06080"
     CDS             complement(2079..3854)
                     /locus_tag="VG754_06080"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HWF18815.1"
                     /translation="MAGKNLGKCNTANPNLLAWVQSQIELCQPDSVFWCDGSEREKDS
                     LTAEAVAQGVLIKLNQKKLPGCYYHRSNPNDVARVEQCTYICTETRDEAGPTNNWVAP
                     GEMYQKLRDLSCGAMKGRTMYVVPYLMGPAGSPMTKVGVELTDSIYVVLSMRIMTRMG
                     NVALEHLGNNDDFNRGLHCMLDVNPERRYICHFPQDNKIISVGSAYGGNVLLGKKCLA
                     LRIGSYLARKQGWMAEHMLILGVQSPKGEKTFVAAAFPSACGKTNFAMLVPPAHFKGW
                     KVWTVGDDIAWMKPGADGMLYAINPEAGYFGVVPGTNSKSNPNAMASMSRDTIYTNVA
                     LTPDLDVWWEGKDGPPPKECLDWRGNKWTPASREKAAHPNSRFAAPMMNNPMLAPEAN
                     DPKGVPISAIIFGGRRSDTMPLVYQAFDWIHGVYIGAMMGSEMTAAASGVIGQVRRDP
                     MAMLPFCGYHMGDYFQHWVTMRKLIKHPPRVFHVNWFRKDERGEFLWPGFGDNMRVLK
                     WIVERCEGRAHADETALGWMPRIKDFDLDEMPGYSREQLERVQSIVPEEWHRELLLQE
                     ELYMKLYSHMPKELVFQRQLLTSRL"
     gene            4130..>5076
                     /locus_tag="VG754_06085"
     CDS             4130..>5076
                     /locus_tag="VG754_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HWF18816.1"
                     /translation="MSLPASREKQADAQSTKCLARSVTEVLAQEPALEAVTFNRQRKT
                     ISVATLGRADVPKITERVTATIQEAQPTDVNRCGLLSGEGDCQTCAVPLTEKERGIIT
                     IKNEGDATTIARVTCPTAPSFWRWRDIPWPKIVPRDVEFMEHADHADEWKAQLAAAIL
                     CGIFALAGHFFKGHPAALYCYLAAYLAGGWFTVEEVWERLRERAIDVHFLMLAVAAGS
                     ASIGAWGEGSTLLFLFSLSGALEHYALGRTQREIRSLFREAPKTAIVLDASGHESEVA
                     VERLRPGMRLLIKPGAQFPVDGEIAKGKTESDESNLTGES"
BASE COUNT         1154 a         1332 c         1499 g         1091 t
ORIGIN      
        1 tttagaagaa aaatttcatt atatttgccg caatccatgg gatggcggag cggcactatc
       61 gacggagtca tacaaatgga tttggactcc cggcggggaa acgggagcag gtgttccaac
      121 cgttccagac aagtcagttg aacgatctgc tcggcgagac gcctcgcaga acacgccgga
      181 ggcgtgtgct ccccaaatct cgggcgcttt cgcgtttcgc ctttatgacg aacagggctt
      241 cccgctcgac ttgactgaat taatggcgcg ggaacgtgga ttgacggtgg acaaagaagg
      301 ttttgacaaa ttgatggaag agcaaaaggc ccgcgcgcgc gccgcgcaga agaaagagat
      361 tatttccgct ggcgaaatag acacgagttc agataccaac tttgtaggct ttgagtcgct
      421 tgaaacaaac gccacagttg ttgcgcacgg cctccttaat ggaaagccgt atctaatgct
      481 caatgaatct ccattgtacg ccgaaatggg ggggcaagtc ggggacactg ggatctttga
      541 agttgacgga aaacggttca cgatactgaa tacaatcaag aagggaaatg tttggttgca
      601 ttttacggct gaaaccgaaa agccaaaaat taacacgaca ggtgtggtaa aggttgacgt
      661 aaaaagacga gaagcaatcc agcgccatca caccgtcacg catatacttc attgggcgtt
      721 gcacgaagtg gcgagccggg aagcttcgca aaagggttcg ttcgtcgggc cggacaaatt
      781 gacattcgac ttcaacagcg cgccgctcat gcccgcgcag atttcggaca tcgaaaagct
      841 tgtcaacgaa cgcatcgtcg aaaattccgg cgtgagctgg acggaagttt cgtatgccga
      901 tgtgaagtcg cgcaaggacg tgatgcagtt tttcggtgac aagtacggtg accttgttcg
      961 cgtggtgcaa atcggcggcg acgcaaacaa attgaacggt tattcgatgg agctttgcgg
     1021 cggtacgcac acgcgcgcga cgggcgagat tggtttgttc cgcatcctta gcgaatcggc
     1081 agttgcggcg ggtgtgcggc gcatcgaggc ggtcgcggga ttgtcggcat atcaggcggc
     1141 gcggcaggaa gcggaattgt tgcgctctgt ggctggacgc gtgaacgcgc cggtcgccga
     1201 attggagaaa aaaatcgagg cgttgctcgc gcagcaaaag gaactggaga aatcattgcg
     1261 cgcggcaaat caaaaggaag cggccggtcg cgcgaaagag cttttggtta aggccgacac
     1321 aagcggcggg gttccatcca tcatcgcaaa tattcccggc gcggatggcg acacgctgca
     1381 aaccatcgtg gactcgttga aaggccagtt caaaggcgtg gtcgttttgg gcggcgcggt
     1441 gaatggcgcg gtggggttgg tggcatcggt ttcaccggag ttcacgaaca aattggcggc
     1501 gggaaaaatc atccaggcga ttgcgccgat tgtcggcggc aagggcggcg gcaagcccga
     1561 taacgcgcgc ggcggcggca aggatgttgc gaagctggac gaagcgcttg caaaagcgaa
     1621 ggaattgtcg aaggtctaaa ttgaacgtga caaaaggcgc gaccaactat tgaattgaat
     1681 tatgaagaat ctctttctat gcgttaccct cgcgatgttc gccatggctt cttccgtgct
     1741 ggcggacagc aacacaactg ccaaagcggc ggctgataaa tcgggcgatt gctgcggctc
     1801 gaataattgt tgcgactcga aagggcattg tgccgacaaa tccaagagtt ccgccaaatc
     1861 ggcttgcagc aaaggaatgc cttccaagag cgtgctgatg agcccgaagg cagcggctgc
     1921 ggcgggaaag tcgtaacgta gtttgacacg ccgttgagta ggaaagggaa gagattgttt
     1981 gccctcatcc ctgcctctcc ctcaggagaa ggtgaacaca tttcaccctg gccagttagc
     2041 cttgattcat cagaggctct tcgcctcgtc tcctttgttt acaaccgcga agtgaggagc
     2101 tggcgttgga agaccagttc cttcggcatg tgcgaatata acttcatgta aagctcctcc
     2161 tgcaaaagca attcgcgatg ccactcttcc gggacgatgc tttgcacgcg ctccaattgc
     2221 tcgcgtgaat agccgggcat ttcgtccaga tcaaaatcct tgatgcgcgg catccagccg
     2281 agcgcggttt cgtccgcgtg agcgcggcct tcgcaccgtt ccacgatcca tttgagcacg
     2341 cgcatgttgt caccgaagcc gggccataga aattcaccgc gctcatcctt gcggaaccaa
     2401 ttcacatgga atactcgcgg cgggtgcttg atcaattttc gcatcgtgac ccaatgctga
     2461 aaataatcgc ccatgtgata gccgcaaaac ggcagcattg ccatcgggtc gcggcgcacc
     2521 tggccgatga cgccgcttgc cgcggcggtc atttccgaac ccatcatcgc gccgatgtaa
     2581 acgccgtgga tccaatcgaa cgcctgataa accagcggca tcgtgtcgct gcgacgtccg
     2641 ccgaaaatga tggcgctgat gggcacgcct ttcggatcgt tcgcttcggg cgccagcatc
     2701 ggattgttca tcatcggcgc ggcgaaacgg ctgttgggat gcgccgcttt ttcgcgtgaa
     2761 gccggcgtcc atttgttgcc gcgccaatcg aggcattcct tgggcggtgg gccgtccttg
     2821 ccctcccacc aaacatccag gtcgggcgtg agcgcaacgt tggtgtaaat cgtgtcgcgc
     2881 gacatggacg ccatcgcatt gggattcgat ttggaatttg ttccgggcac gacaccgaaa
     2941 taacccgctt caggattgat cgcgtaaagc atcccatccg cgccgggctt catccatgcg
     3001 atatcgtccc cgacagtcca aactttccag cccttgaaat gcgcaggcgg cacgagcatg
     3061 gcgaaatttg tcttgccgca ggcgcttggg aacgcggcgg caacgaatgt tttttcgcct
     3121 ttcggagatt gcacgcccaa aatgagcatg tgttcggcca tccagccttg tttgcgcgcg
     3181 agatacgaac cgatgcgcaa tgccagacat tttttgccaa gcagcacgtt gccgccataa
     3241 gcggacccga cggaaatgat tttgttgtcc tgcggaaaat gacagatgta acgccgctcc
     3301 ggattaacat ccaacatgca atgcaagccg cggttgaaat catcgttgtt gccgagatgt
     3361 tcaagcgcga cgtttcccat ccgcgtcatg atgcgcatgc tgaggacgac gtaaatggaa
     3421 tcggtgagtt caacgccaac cttggtcatg ggcgatccgg caggtcccat gagataaggc
     3481 acgacataca tcgtacggcc cttcattgcg ccgcagctaa ggtcgcgaag cttttgatac
     3541 atctcaccgg gcgcgaccca gttgtttgtg ggaccggctt cgtcccgcgt ttcggtgcag
     3601 atgtaagtgc attgctcgac gcgggcgaca tcgttgggat tcgagcgatg ataataacag
     3661 cccggaagct tcttttggtt caacttgatc aagacgccct gcgcgaccgc ttcggcggtg
     3721 agagaatctt tttcgcgctc ggagccgtcg caccagaaca cgctgtcagg ctggcaaagc
     3781 tcgatctggc tttggaccca tgccagcagg ttcggatttg cggtgttgca cttgcccaaa
     3841 tttttaccgg ccataatttc ttcaaatgaa atcacccgtt taattgacct tgttaaagta
     3901 ccgcgttgag cgtggtcggg caaggtctta gaaaataccc aagtacccac gaccaatatc
     3961 gaaataccca aagaaatccg aagcacgaaa tcctcagaac ttgaagcgcg ggaattcgtc
     4021 acattttgga acttgacctt gagatttctt tgggtgcttg tttatttgcg cctttccgtt
     4081 cgtttgcaat gagggtaaaa tgatgcagat ttaggaatat tttgacccga tgagcctgcc
     4141 cgcatccaga gaaaagcaag ccgacgcgca gtcaaccaaa tgcctggcgc gttcggtgac
     4201 ggaggtgttg gcacaggaac cggcgttgga agctgtcacg ttcaaccggc agcgcaagac
     4261 catttctgtc gcgacattgg gccgcgcgga tgtccctaaa atcaccgagc gcgtcacggc
     4321 caccatccag gaagcgcaac cgacggatgt gaatcgttgc ggtttgcttt ccggcgaagg
     4381 cgattgccag acttgcgccg tgccgttgac cgaaaaggag cgcggaatca tcaccatcaa
     4441 aaacgaaggc gacgccacga ccatcgcccg ggtgacttgt ccgaccgcgc cgagcttctg
     4501 gcgctggcgg gacattccgt ggccgaaaat cgtgccgcgg gacgtcgagt tcatggagca
     4561 cgccgaccat gctgatgagt ggaaagcgca actcgcggct gcgattttgt gcggcatctt
     4621 cgcattggcc gggcattttt tcaaaggaca tccagcggcg ctctattgct atctcgcggc
     4681 gtatctcgcg ggcggatggt tcaccgtgga ggaagtttgg gaacggttgc gcgaacgcgc
     4741 gattgacgtg cattttctca tgctggcggt cgcggcgggc agcgcgagca tcggcgcgtg
     4801 gggcgagggt tcgacgctgt tgtttctgtt ttcgttgtcc ggtgcgttgg aacattacgc
     4861 cctcgggcgg acgcagcgcg aaatccgttc attgttccgt gaagcgccga agacggccat
     4921 cgtgcttgat gcttccggtc acgagagcga agtggcggtc gaacggttgc gtccgggaat
     4981 gcgcctgctc atcaagcctg gcgcgcagtt tcctgtggac ggcgaaatcg cgaagggcaa
     5041 aaccgaaagc gacgaatcca atctcaccgg agaatc
//
LOCUS       DASZMM010000253         5294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3405607, whole genome shotgun sequence.
ACCESSION   DASZMM010000253 DASZMM010000000
VERSION     DASZMM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405607"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VG754_06090"
     CDS             complement(<1..966)
                     /locus_tag="VG754_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF18817.1"
                     /translation="MTFAHPYFLLLLLLLPVLAWLKGKRGRQPAFLYSSVQLVKGIIG
                     ITRSNAGGMLLKLRWLALACFIVALAQPRMILSESKVHASGVDIVVALDLSGSMAAED
                     DGFILNGQQATRFMIAKDTLKKFIAKRPNDRLGLVIFATHAFVVVPPTLDHDFLSANL
                     ERLELNMEGFDDHSTAIGSALSTSLNRLRDLQSKSKIVILMTDGENNAGKIPPLTAAE
                     AAQALGVKVYTIGVGTRGEARIQQMDIFGNRHYVKIPVDVDEDTLTKIANMNGGKYYR
                     ADSSATLNKIYNDIDRLEKTDVEVKKYTQYNELFSWVILPGLFFVL"
     gene            complement(963..1538)
                     /locus_tag="VG754_06095"
     CDS             complement(963..1538)
                     /locus_tag="VG754_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18818.1"
                     /translation="MNTNLPTQAGTGALPHAADIRPPKPPLEIPNGWLWFWGVLIALA
                     FVAACYFAWRYWRKKAATPILASIVPPHERARLKLQEALALISQPRPFCILVSDTVRL
                     YLEERFHFHAPERTTEEFLHELQSSDLLLPDQKTSLGEFLSMCDMVKFARYEPGTTEL
                     EALHQSAVRLIDETEPQPDPAENAIAEPAQS"
     gene            complement(1535..2494)
                     /locus_tag="VG754_06100"
     CDS             complement(1535..2494)
                     /locus_tag="VG754_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWF18819.1"
                     /translation="MIPREILKKIRQIEIRTNRLVNETLGGQYHSVFKGQGMNFEEVR
                     EYQPGDEVRSIDWNVTARMNHPFVKKFVEERELTLMLVVDVSGSGLFGSGEQSKRELA
                     AEIASVLAFSAIRNNDKVGLILFTEEVEKFIPPRKGRRHVLRVIREILFFEPKRRGTN
                     LNHALEFLTRVTPHRAIAAVVSDFLGQNITEPHQGGATRRSSGPMLAQSLAQASFTAL
                     RQANRRHDVVAIQIADRYELELPPLGRLILKDAETGEVVEVNTGDARKREAFMQRQMR
                     AQAELLRVFRSAKIDSILLRTDQSYTSALGRFFETREKRRLHG"
     gene            2534..2767
                     /locus_tag="VG754_06105"
     CDS             2534..2767
                     /locus_tag="VG754_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HWF18820.1"
                     /translation="MSATEILKAAKALPVEEQIELAQELWYGIAEKGCDPDLTPEQAA
                     ELDRRLADFERNPGEGIPLEQVKAEMKKHFGWE"
     gene            2764..3066
                     /locus_tag="VG754_06110"
     CDS             2764..3066
                     /locus_tag="VG754_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF18821.1"
                     /translation="MTFVFQAPAKLEFFAAIAWYENQQLGLGQDFAREVIETLRRARL
                     RPELFQKVRGRARKVRLRRFHAYSIYFAIKQDCFSVLAIFHGARNPKDLLRRFDKD"
     gene            complement(3200..3385)
                     /locus_tag="VG754_06115"
     CDS             complement(3200..3385)
                     /locus_tag="VG754_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016514546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWF18822.1"
                     /translation="MKRRELIRHLTSHGCRLVREGANHSWWENAALNRRSAVPRHTEI
                     NRFLARKICRDLGIAEL"
     gene            complement(3382..3594)
                     /locus_tag="VG754_06120"
     CDS             complement(3382..3594)
                     /locus_tag="VG754_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF18823.1"
                     /translation="MKNRYTAVIKQDSGWWIGWIEEIPGVNSQGKTKAELKKNLRSAL
                     SEALEFNREDALQAAEADYEEAVIMA"
     gene            complement(3614..4603)
                     /locus_tag="VG754_06125"
     CDS             complement(3614..4603)
                     /locus_tag="VG754_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWF18824.1"
                     /translation="MNTGISAINNAVQESSAFVRPLFNEIGKVIIGQTYLVERLTIGL
                     LANGHVLLEGVPGLAKTLSVKSLASCLSVKFSRLQFTPDMLPADVIGTQIYNPQSGGF
                     STRRGPIFANLVLADEINRAPAKVQSALLEAMQEKQVTIGDQTFKLEEPFLVLATQNP
                     IEQEGTYPLPEAQVDRFMLKLKIGYPTRAEERQILDLMAHTTNLPNVVPVVTPQQIFE
                     ARRIINDIYIDEKVKDYIVDLVCATREPETYKIQLKDFIQLGASPRATIALTLAAKAF
                     AFLRGRGYVTPQDVKSIGMDVLRHRVTITYEAEAENKTSETVIQKIFDELPVP"
     gene            4628..5032
                     /locus_tag="VG754_06130"
     CDS             4628..5032
                     /locus_tag="VG754_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18825.1"
                     /translation="MDKKLENPFSKSNAAPVDLGLLSSPMTDKCSNAGRNFNQAVNKK
                     QRVIMAILTLVLIGIGRIVALIGGIMFLIVAFQESVLWGIGCLLVPFVSLIFLVLHWA
                     EAKKTFFIQLAGLALVFIGVLIGGHGHMPPPH"
     gene            complement(5057..>5294)
                     /locus_tag="VG754_06135"
     CDS             complement(5057..>5294)
                     /locus_tag="VG754_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HWF18826.1"
                     /translation="AQGRTHRAVVNIGSRPTLQNPSPQLRVEAHLLDFTGDLYGQEME
                     IIFLEKLRDEMKFSSLDALKAQITRDIAEARRRF"
BASE COUNT         1167 a         1372 c         1493 g         1262 t
ORIGIN      
        1 cagcacaaaa aacaaacccg gcaaaatgac ccacgaaaaa agctcgttgt attgcgtgta
       61 ttttttcact tccacgtcgg tcttctcaag gcggtcaatg tcattgtaaa tcttattgag
      121 cgtcgccgag ctgtcggcgc gataatattt tccgccgttc atgttcgcga tcttggtcag
      181 ggtgtcttca tccacgtcaa caggaatttt cacgtaatgc cggttgccga aaatgtccat
      241 ctgctggatg cgcgcctcgc cacgcgtgcc gacgccgatg gtatagacct tgacgcccaa
      301 cgcctgcgca gcctcggcgg cggtgagcgg cggaattttg cctgcgttgt tttcaccatc
      361 ggtcatcagg atgacgatct tgcttttcga ctggagatcg cgcaaccggt ttaacgaagt
      421 ggagagcgcc gagcctatcg cggtggaatg atcgtcaaaa ccttccatgt tgagttcgag
      481 ccgctccagg ttggcggaca aaaaatcgtg gtcaagggtc ggtggcacca cgacaaacgc
      541 atgcgttgcg aaaatgacga ggccgagccg gtcattcggg cgcttggcaa tgaatttttt
      601 caacgtgtcc ttcgcgatca tgaagcgcgt cgcctgttga ccgtttaaaa tgaagccgtc
      661 gtcctcggcg gccatgctgc cggataaatc gagcgccacg acgatgtcca cgccgctcgc
      721 gtggaccttg ctttcgctca aaatcatccg cggttgcgcg agcgcgacga tgaaacaagc
      781 gagcgcgagc cagcgcagtt tcagcaacat gcccccggca ttggagcggg tgatgccgat
      841 gatgcctttc accaattgca ccgaggaata aagaaacgcc ggttgacgcc cccttttgcc
      901 cttgagccaa gcgagcaccg gcaacaggag cagcaacagt aaaaaatatg gatgcgcgaa
      961 ggtcatgatt gcgccggttc ggcaatggca ttttccgcgg gatcaggctg cggttcagtt
     1021 tcgtcaatca gccggacagc ggattgatgg agcgcttcca gttccgttgt tccgggttcg
     1081 tagcgggcga atttgaccat gtcacacatc gaaagaaatt cgccaaggct cgtcttctgg
     1141 tccggcagca ggagatcgga agattgcagt tcgtgcagga attcctcagt cgtgcgctcc
     1201 ggcgcgtgaa aatgaaaacg ctcttcaagg taaagacgga cggtgtcgga caccaagatg
     1261 caaaacggtc tcggttgcga aatcaacgcg agcgcttcct gcagcttcaa gcgggcgcgt
     1321 tcatgcggtg ggacaatgga agcgagaatt ggagtcgcag cttttttccg ccaataacgc
     1381 catgcaaaat agcacgccgc cacgaacgcc agcgcgatga gcacgcccca aaaccaaagc
     1441 cagccgttcg ggatttccag cggcggcttg ggcggacgga tgtccgcggc gtggggaagc
     1501 gcgcctgtgc cagcttgagt tggaagattg gtgttcatcc gtgcaatcgg cgtttttcgc
     1561 gcgtttcaaa aaaacggccc agcgcggaag tataggactg gtcggtgcgc aaaaggatgg
     1621 aatcaatctt cgcggaccga aagacgcgca acaattccgc ctgcgcccgc atttggcgtt
     1681 gcatgaaggc ttcgcgcttg cgggcgtcgc cggtgttgac ttccaccact tcgcccgttt
     1741 ccgcatcttt caaaatcaaa cggcccaacg gcggcaattc aagttcataa cggtcggcga
     1801 tctggatggc cacgacgtcg tgccggcgat tggcctgccg caacgcggtg aacgaagctt
     1861 gcgcgagcga ttgcgccagc atcggcccgg aagagcgacg cgtcgccccg ccttgatgcg
     1921 gctccgtgat gttttgtcca agaaaatcgg aaaccaccgc cgcaatggcg cgatgcggcg
     1981 tgacacgggt gagaaattcg agcgcgtgat tcaaatttgt gccgcggcgc ttcggctcga
     2041 agaaaagaat ttcgcggatg acgcgcagca cgtgacgacg tcctttgcga ggcggaatga
     2101 atttttccac ttcctcggtg aaaagaatga gacccacctt gtcattattg cggatggcgg
     2161 agaaggcgag gaccgaagca atttccgccg cgagttcgcg cttcgattgc tcgccggagc
     2221 cgaacaaacc ggagccgctc acatccacta ccagcattaa ggtcagttca cgttcctcga
     2281 cgaatttttt tacgaacgga tggttcatcc gcgcggtgac gttccagtca atcgagcgca
     2341 cttcgtcgcc cggttgatat tcgcgcacct cctcaaaatt catcccctgg cccttgaaga
     2401 cgctgtgata ctggccgccg agtgtttcgt tcacgagccg gttcgtgcga atctcgatct
     2461 ggcggatttt tttgaggatt tcgcggggga tcatgggctt gcaaaagcga tttgggcgga
     2521 ataatctgct ttcatgagcg cgaccgaaat tttgaaagcg gcaaaagccc ttcctgttga
     2581 agagcagatc gaactggcgc aggagctttg gtatggcatt gccgaaaaag ggtgcgaccc
     2641 cgatttaaca cccgaacagg ccgcggaact tgacagacgg cttgccgact ttgagagaaa
     2701 tcccggagag ggaatcccat tggagcaggt caaggccgaa atgaagaaac atttcggctg
     2761 ggaatgacat tcgtttttca agctcctgcc aagctggaat tttttgcggc cattgcgtgg
     2821 tacgaaaatc aacaacttgg attgggacaa gatttcgccc gcgaggttat tgaaacattg
     2881 agacgagcgc gactgcgacc tgaacttttt cagaaagttc gtggcagggc gcgtaaagtt
     2941 cggcttcgca ggtttcacgc gtacagcatt tactttgcga tcaagcaaga ctgcttttcg
     3001 gtattggcaa tcttccatgg tgctcgcaat ccgaaagatt tgctgcgtag gtttgataaa
     3061 gactagcacc ccattttcgt tcacgagccg gttcgtacga atctcgatct ggcggatttt
     3121 cttgaggatt tcgcggggaa tcatttaggc ttcaaaatat tttccattcc attcgtaaat
     3181 ggctcgagag ctttccgcat catagttcag cgatgcccag gtcccgacag attttgcgcg
     3241 cgagaaaacg attgatttcc gtatggcgag ggactgcgga gcgtcggttg agcgcggcat
     3301 tttcccacca tgaatgattt gcgccttcgc gaaccagccg gcagccatgg gaggtcaaat
     3361 gacgaatcaa ctcgcgtctc ttcacgccat tataaccgct tcttcgtaat cagcttccgc
     3421 ggcttgcaat gcgtcttcac gattaaactc cagcgcttca gacaatgccg aacgcaaatt
     3481 ctttttcagt tcggccttgg tctttccctg gctgttaacc ccggggattt cctcgatcca
     3541 gccgatccac caaccggaat cttgtttgat gacagcagta tatctgtttt tcataatttt
     3601 aatatttcca atgtcacggc accggcaatt cgtcaaaaat cttttggatg accgtctcgc
     3661 tcgttttgtt ctcggcttcg gcttcgtaag tgatggtcac tcgatgacgc agcacgtcca
     3721 tgccgatgct cttgacgtcc tgcggcgtca cgtagccgcg accgcgcaga aaggcaaaag
     3781 ccttggccgc gagtgtcaac gcgatggtcg cgcgcggtga agcgccgagc tggatgaaat
     3841 ctttcaactg tattttgtaa gtctccggct cgcgcgtcgc gcaaacgagg tccacaatgt
     3901 aatccttcac tttttcatca atataaatgt cattgatgat gcggcgcgcc tcgaagattt
     3961 gctgcggcgt aaccaccggg acaacgttcg gcaaattcgt ggtatgggcc atcaggtcga
     4021 gaatttgccg ttcctcggcg cgcgtaggat aaccgatttt caatttgagc atgaaccggt
     4081 caacttgcgc ttcgggcagg ggataggtgc cttcctgttc gatgggattt tgcgtggcaa
     4141 gcacgagaaa cggttcttcg agcttgaagg tttggtcgcc gatggtgacc tgtttttcct
     4201 gcatcgcttc gagcaaggcg ctttggacct tggcgggggc gcggttgatt tcgtcggcga
     4261 gcaccagatt ggcaaaaatg gggccgcgcc gcgtgctaaa accgccggat tgcggattgt
     4321 agatctgcgt gccgatgacg tcggcgggga gcatgtccgg cgtgaattgc aatcgcgaga
     4381 acttgacgct caagcacgat gccagcgact tgacggagag cgtcttggcc aaccccggca
     4441 cgccttcaag caggacgtgg ccgttggcga gcaggccgat ggtcagccgc tcgaccagat
     4501 aggtttggcc gatgatgact ttgccgattt cgttgaataa tggccgcacg aaggcgctgg
     4561 actcctgcac ggcgttgttg attgcgctta tgcctgtatt catcttgggc ttagatatta
     4621 cttatggatg gacaaaaaac tagaaaatcc gttcagcaaa agcaatgcgg ctccggttga
     4681 tttgggcttg ctttccagtc ccatgacgga taaatgctcg aatgcagggc gtaatttcaa
     4741 ccaggcagtc aataaaaaac aaagggtaat tatggcgatt ctaactttgg ttctgatagg
     4801 cattgggagg atcgtggcat taatcggagg aataatgttt ttgatcgtcg catttcaaga
     4861 aagcgtactc tggggcatcg gctgcctttt ggtccctttt gtaagcctca tttttctggt
     4921 gctccattgg gcagaggcaa aaaaaacatt tttcattcaa cttgctggtc tggctttggt
     4981 tttcatcggt gtgttgattg gaggtcatgg gcacatgcct cctccccatt aaacggcggg
     5041 tctcgactcg tagattctaa aaccgtcttc tcgcctccgc aatgtcgcgt gtaatttgcg
     5101 ccttgagagc gtcgagggac gagaatttca tttcatcacg cagtttttcc agaaagatga
     5161 tttccatttc ctggccgtaa aggtcgccag tgaaatccag cagatgcgcc tcgacgcgga
     5221 gctgtggcga gggattttgc agggtggggc gcgagccgat gttcaccacc gcgcgatgcg
     5281 tccggccttg cgcg
//
LOCUS       DASZMM010000254         5109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3406111, whole genome shotgun sequence.
ACCESSION   DASZMM010000254 DASZMM010000000
VERSION     DASZMM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3406111"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VG754_06140"
     CDS             <1..706
                     /locus_tag="VG754_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HWF18827.1"
                     /translation="LATIYLWRAVFGSKPGGETVAGYTLMQMTSYYLIVTLVDVLTAV
                     NEDDWQIASDIKDGNISQFLVKPIDYLRYRLCLFFSGRLIYLLVTAAPLAVFIFCLRK
                     YFVLPEPMMLGWFLLSVFLTALLQFFTSYAMAMLAFWVLEVSTFIFILFAFEYLASGH
                     LFPLDILPPTIAKLMNFTPFPYQMYFPVSIYMGRITGTQLARGLVIQALWVFAAYLIA
                     RFAWNRGIRKYSAVGG"
     gene            800..1642
                     /locus_tag="VG754_06145"
     CDS             800..1642
                     /locus_tag="VG754_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HWF18828.1"
                     /translation="MPNSEIQTDSNPRRPFFLRRYLGIYSALWKNSVAREMMFKSNFL
                     LWIFVEVLWFALQLTFFGVIYLHTDRIGTWTTWQVVLLVGASQFIQQIFQSFFLINCA
                     NLSELVRSGKLDFLLLLPVNTRFIISLRQVDLGGFVNAGLALVVMGFAITKLPAPPSF
                     LQILCFPLLCVAGILIHYSLMFLLAGISFWTVRAQGIVWGYYNLFNIARMPDEAFKGV
                     FKVVFTFIIPMLLVSNVPARVLAGKLESPVSLLLLIGMAAVCMLVSELGWRQSLKRYT
                     SASS"
     gene            1801..2637
                     /locus_tag="VG754_06150"
     CDS             1801..2637
                     /locus_tag="VG754_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWF18829.1"
                     /translation="MPSKILTDAQVAAFQRDGFVLVASLFDGEETDILRKTAKADAVM
                     KKHAYDLKDGEGGATKLVLWNNAGEDVYGTVARCPRIVHAMEQLLGDEVYHYHSKMSI
                     KEPLTGGAWSWHQDYGYWYMNGCLFPDMASAFIAVDPNTRENGCLQVLKGSHLMGRIE
                     HGKFGDQTGADPERVENAMKIMELVHVEMNPGDVLFFHSNTLHRSDQNKSPHPRWSMI
                     CCYNTRHNNPYKASHHPFYEKLVTVPDSALKEMGVKLFAEKTDFWDPNHDKTVAGGAQ
                     RK"
     gene            2770..3099
                     /locus_tag="VG754_06155"
     CDS             2770..3099
                     /locus_tag="VG754_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HWF18830.1"
                     /translation="MKKITTSLLAGVVALMLLALTTPAFAREVETITGEGKCAKCALH
                     QTEKCQNAVEVQKDGKTVTYYLTGKVSKDFHENLCKESKKVTATGDVSEKDGKMTMKV
                     TKIEEVK"
     gene            3227..3490
                     /locus_tag="VG754_06160"
     CDS             3227..3490
                     /locus_tag="VG754_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18831.1"
                     /translation="MLPKLRRRGIMDSGMTTEEFEIVAFLKLYPDSYFARKEIARKAR
                     SRDDYEANPHWAAAPLASLVLQGQIVQNDNGQYKINTEAVDKD"
     gene            3544..>5109
                     /locus_tag="VG754_06165"
     CDS             3544..>5109
                     /locus_tag="VG754_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWF18832.1"
                     /translation="MDLNIHPPLPKRKDFRIGILGSGFIVDECHLVAYRKAGFNPVAI
                     ASRNRANATEVATAHNISKVFDTYDDLLADPTIEVLDIAVPPNALLPLIRKVCDARIV
                     KGVLAQKPLGMNYAEALEMVTYCERAGITLAVNQNMRFDQSVRAAKTLLTNGTIGEPV
                     IATIEMRGIPHWQPWQAELGWATLRIMSIHHLDTFRYWFGSPQRIFCSTRPDPRTKFS
                     HTDGICVYILEYANGLRCIAIDDTWTGPAKEGCPSDNYIKWRIEGLNGLAIGDIGWCR
                     DPYTTPSTIRYAAKGQQNFESPIWPESWFPDAFIGTMADLLIALENNSQPAISGRDNL
                     KTMQLVEGAYRSARFHYAIPIIDNPLEPQTSQSPLRTHTQSKWSDISARIGAAMRDPR
                     RNNFTPRAVQVLALARKEADRLNHNFVGTEHVLLGLIVLGQGCAVTVLAKLGLNLENV
                     RNEVEKQIGVGPDQKVVGNIPYAPRVKKVLALAARQAKNLHHRFVGTEHILLGLLEEG
                     DGVAARVLRTFNVD"
BASE COUNT         1175 a         1396 c         1306 g         1232 t
ORIGIN      
        1 gctggccacg atttatctct ggcgcgcggt ttttggcagc aagccgggcg gagaaactgt
       61 cgccggttac acattgatgc aaatgacttc ctattatctc atcgtcaccc tggtggacgt
      121 gctgaccgcg gtgaacgagg atgattggca gatcgcctcg gacatcaagg acggcaacat
      181 cagccaattt ttggtgaagc cgattgatta tctccgttac cggctctgcc tgtttttttc
      241 aggacgattg atttacctgc tggtcactgc cgcgccactg gcggtgttca tcttttgcct
      301 tcgaaaatat ttcgttctgc ccgaaccgat gatgttgggc tggtttttgc tgtcggtgtt
      361 cctcacggcg ttgctccagt ttttcacatc gtatgcgatg gcgatgctgg cgttttgggt
      421 gctcgaagtt tccacgttta ttttcatcct gttcgcgttc gaatacctcg ccagcggtca
      481 tctttttccg ctcgacattt tgccgcccac gattgccaag ctgatgaatt tcacgccgtt
      541 cccatatcaa atgtattttc cggtgagcat ttacatgggt cggatcaccg gaactcaatt
      601 ggcgcgcggg ctcgtcattc aggcgttgtg ggtttttgcc gcttacttga tcgcccgttt
      661 tgcatggaac cgcggcatcc gcaaatactc ggcggtcgga ggatgactgt gcaatttcaa
      721 agttcaaatt tacaatttca aattaagagg cttgtaaacc gcctcctaca acttgctcca
      781 accctcaatt ttttgacgca tgccaaattc tgaaatccaa accgattcaa atccacgccg
      841 gccctttttt ctccggcgct atctcggcat ttactcggcc ctctggaaaa attccgtggc
      901 ccgtgagatg atgttcaagt ccaatttcct gctctggatt tttgtcgagg tgctctggtt
      961 cgcattgcag ctgacatttt tcggggtcat ttatctgcac actgaccgca tcgggacttg
     1021 gacgacgtgg caggtcgtgc tgctggttgg cgcaagccag ttcatccaac agattttcca
     1081 atcgtttttt ctcatcaatt gcgcgaatct ttccgaactc gtccgctcgg gcaaactcga
     1141 ttttctgctg ttgctgcccg tgaacacccg gttcatcatt tcattgcggc aggtggactt
     1201 gggcgggttt gtgaatgccg gcctggcact ggtcgtgatg gggttcgcga tcacaaaact
     1261 gcccgcaccg ccgtcattcc tgcaaattct ctgttttcct ctgctctgcg tggcggggat
     1321 tctcattcat tattcgctga tgtttctgct ggctggcatc agcttctgga cggtgcgcgc
     1381 gcaagggatt gtttggggtt actacaatct gttcaacatc gcgcggatgc cggatgaagc
     1441 gttcaaaggt gtgttcaaag tggtgttcac cttcatcatt ccgatgttgc tggtctccaa
     1501 tgttccggcg cgtgtgctcg caggcaaact cgaatcacct gtgtcgctct tgctgttgat
     1561 cggcatggcg gcggtctgca tgctggtttc agaattgggc tggcggcaat cgttgaaacg
     1621 ctacaccagc gcgagttctt aaaattgggt ggaagccaac gtgaggaggc tctcatctga
     1681 tttgaatttc aaatttcaga ttgaaataca aacggagtct gccgaaaaat ggcggacttg
     1741 cgccgggacg cgctgcgcat tacaatccgt ttacaagcca gccaaaaacg taacagaatt
     1801 atgccatcga aaattctcac cgatgcccag gttgccgcat tccaacgcga cggtttcgtg
     1861 cttgtcgcct cccttttcga cggcgaagaa acggacatcc tgcgcaaaac cgccaaggcc
     1921 gatgccgtga tgaaaaaaca cgcctacgat ctgaaggatg gcgaaggtgg cgcgaccaag
     1981 ctggtgctct ggaacaacgc gggcgaagac gtttacggaa ctgttgcgcg ttgcccgcgc
     2041 atcgtccatg cgatggaaca attgctcggc gatgaagtct atcattacca ttccaagatg
     2101 agcatcaagg agccattgac cggcggcgcg tggagctggc accaggatta tggttattgg
     2161 tacatgaacg gctgtctttt tcccgatatg gcctcggcat tcatcgcggt tgacccgaac
     2221 acgcgcgaaa acggctgcct tcaagtcctc aaaggctcgc atctgatggg gcgcattgaa
     2281 cacggcaaat tcggcgatca aaccggcgcc gaccctgaac gcgtcgaaaa cgccatgaag
     2341 attatggaac tggtccatgt cgagatgaat cccggcgacg tgcttttttt ccacagcaac
     2401 acgctgcatc gctccgacca gaacaaatcg ccgcatcccc gctggtccat gatttgttgc
     2461 tacaataccc gccacaataa tccttacaag gcgtcgcatc atcctttcta tgagaaactt
     2521 gtgacggtgc cggacagcgc cctgaaggag atgggcgtga aattgttcgc tgagaaaacc
     2581 gacttttggg acccgaacca cgacaaaacc gtcgctggcg gcgcgcagcg aaaatgattg
     2641 caattctatc ccgctgggag cgtgtcattt gcgttcttct ttgccccttg acaaacagga
     2701 tgtataggcg aaaattatcc ccgttcaata gcagaaatta aactaacagt aattccacaa
     2761 attcaatcta tgaaaaaaat cacgacctca ctcctcgctg gcgtcgttgc gttgatgttg
     2821 ctcgcattga ccacgcctgc ttttgcccgc gaagttgaaa ccatcaccgg cgaaggcaaa
     2881 tgcgccaaat gcgcgttgca ccagaccgaa aagtgccaga atgccgtcga agtccagaag
     2941 gacggcaaaa ccgtcaccta ttatctgact ggcaaggtca gcaaggattt ccatgaaaac
     3001 ctttgcaagg aatccaagaa ggtcactgcc accggcgacg tgtcggaaaa agacggcaag
     3061 atgaccatga aggttacgaa gattgaagaa gtcaaataac gctttgattt aaccaacagt
     3121 tttggtttgt ctgcgcccgc ttttcaggtc gaaagaccgg aaagcgggca ttttgttttc
     3181 gtagctgaaa acgtgagttt tcagccttaa aaagaacggc cattttatgc ttcctaagct
     3241 gcggcgaaga ggcatcatgg acagcgggat gactaccgag gaatttgaaa tcgttgcgtt
     3301 tctgaaacta tacccagaca gttattttgc ccgcaaggaa atcgcccgca aggcgcgcag
     3361 tcgtgatgat tatgaagcca atcctcattg ggccgccgcg ccgctcgcct cgctcgtcct
     3421 ccagggtcag atcgtgcaga acgacaacgg ccaatacaaa atcaacaccg aggccgtgga
     3481 caaggattag ccggtgttga gaaaaaagtt tgctcgccac gttgactgca aaacatcctg
     3541 tttatggacc tcaacatcca tcctcccctg cccaagcgga aagattttcg catcggcatt
     3601 cttggcagcg ggttcatcgt ggatgaatgc catctcgtcg cttaccggaa ggccggcttc
     3661 aatccagttg ccatcgcttc gcgcaaccgc gccaacgcca cggaagtcgc caccgcgcac
     3721 aacatctcga aggtgtttga cacctatgac gaccttctcg ccgatccaac catcgaagtg
     3781 ctcgacatcg ccgtaccgcc caacgcgctt cttccgttga tccgcaaagt ctgcgacgcg
     3841 cgcatcgtca aaggcgtcct tgcgcaaaaa ccgttgggca tgaattacgc cgaggcgctg
     3901 gaaatggtga cttattgcga acgcgccggc atcacgctcg ccgtgaacca aaacatgcgc
     3961 ttcgaccaat ccgtgcgcgc cgcaaaaaca ttgctgacca acgggaccat cggcgagccg
     4021 gtgattgcga ccattgagat gcgcggcatc ccgcattggc aaccctggca ggcggaactc
     4081 ggctgggcca cgttgcgcat catgtccatc catcatctcg acacgtttcg atattggttc
     4141 ggctcgccgc aacgaatttt ttgcagcacg cgacccgacc cgcgcacgaa attttcgcac
     4201 accgatggca tctgcgttta cattttggaa tacgccaacg gcctgcgctg catcgccatt
     4261 gatgacacct ggaccggccc ggccaaggaa ggttgcccgt cggacaacta tatcaaatgg
     4321 cgcatcgagg gcctgaatgg ccttgccatc ggcgacatcg gctggtgccg cgatccttac
     4381 accacgccat caacgattcg ttacgccgca aagggccaac aaaatttcga atcacccatc
     4441 tggcccgaaa gctggtttcc ggatgccttc atcgggacta tggccgattt gctgatcgct
     4501 ttggaaaata attcgcaacc agccatcagc ggacgcgata atttgaagac gatgcaatta
     4561 gtagagggag cttatcgcag tgctcgattt cactacgcca tcccgattat tgacaatcca
     4621 ctggaacctc aaacaagcca atcaccttta agaactcata ctcaatccaa atggtcggac
     4681 atttcagcgc gcatcggagc ggcgatgcgg gatccgcgac gaaacaattt cactccacgt
     4741 gcggtgcagg ttcttgcgct cgctcgcaaa gaggcggacc gtctcaacca caactttgtc
     4801 ggcacagaac atgtgctgct gggtttgatt gtgctgggcc agggttgtgc cgtgacggtg
     4861 ctcgcaaaac ttgggttgaa cctggagaac gtcaggaacg aagtcgaaaa acaaattggc
     4921 gtcggtcccg atcaaaaagt cgtcggcaac attccttacg ctccccgcgt taaaaaggtc
     4981 cttgcgctcg ccgctcggca ggcgaaaaat cttcaccata gatttgtcgg cacagaacac
     5041 atcttgcttg ggcttctgga agaaggcgac ggcgttgcag cgcgtgtatt gagaactttc
     5101 aatgtggat
//
LOCUS       DASZMM010000255         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4228130, whole genome shotgun sequence.
ACCESSION   DASZMM010000255 DASZMM010000000
VERSION     DASZMM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4228130"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /locus_tag="VG754_06170"
     CDS             <1..1890
                     /locus_tag="VG754_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWF18833.1"
                     /translation="AVKRMAEHLQANGRLVLFAEGRLSRTSTLMKLFEGTGFLLHKTN
                     AKVITCYLRGAQHLPFSPNPSPKKWFPNISIHFSEVLTPPKIENASTVQARQKLTMWL
                     RDQMVNQQFNVEMELGAKDVLSAIVAVARQRPRQFILEDVSRKPLTYRRLLAGTDALS
                     QPLQRILMPNESRVGVLMPNVSAMPVILLSLWRLGKVPAILNFSTGAATMLTCSQLAG
                     LKQIVTSRAFLERARLKIEPLIEAGIQFVYLEDLGKEISGAQKFLSLLRMTFNPGSLT
                     PKDDSRLSSTPQSAVILFTSGSEGVPKGVELSHTNLLANMRQMLAVTDITDRDRLFNC
                     LPMFHSFGLTVGTLLPLVRGIYVFVYPSPLHYRVVPALFYETDSSILLSTNTFLNGYA
                     RKGNPYDFRSMRYLFAAAEKLQEATASTWAQKFGVRILEGYGATECSPCVSVNTPLSP
                     KYGSVGRLLPGMECRLEKIEGVEEGGELFVRGPNVMIGYLNADANAKFKALNGWYDTG
                     DIVSVDEEGYLSIRGRLKRFAKVSGEMVSLTAVEDALAGAFPQYGLRCQVAVVTRPDE
                     GKGEALIAVTNEPKLQLEEIRAVIKAKGLSNLSAPREIKVVREIPKLGTGKINHRELA
                     TMINK"
BASE COUNT          434 a          521 c          563 g          447 t
ORIGIN      
        1 gcagtgaaac gcatggcaga gcatttgcag gcgaatgggc gtctcgttct ttttgccgaa
       61 ggccggttgt cgcgcaccag cacgttgatg aagctctttg aaggcaccgg ttttttgctg
      121 cacaaaacca atgccaaggt catcacctgc tatttgcgcg gcgcgcaaca tctgccgttc
      181 tcgccaaatc cctcgcccaa aaaatggttt cccaacatct ccatccattt cagtgaagtg
      241 ctcacgccgc cgaaaatcga aaacgccagc acagtgcagg cccggcagaa attgaccatg
      301 tggctgcgcg accagatggt gaatcaacaa ttcaatgtcg agatggaact tggcgcgaag
      361 gatgttttgt cggcgattgt cgccgtcgcg cgacaacgcc cgcggcaatt catcctggag
      421 gatgtttcgc gcaagccgct cacgtatcga cgtctgctgg ccgggactga cgcgctttcg
      481 caaccgttgc aacgcatctt gatgcccaat gaatcgcgtg tcggtgtgct catgccgaac
      541 gtgagcgcca tgccggtcat tttgctcagc ttatggcggc ttggcaaagt ccctgccatt
      601 ttgaattttt cgactggcgc ggcaacgatg ctcacctgtt cgcaactggc cggcttgaaa
      661 caaatcgtca cttcgcgcgc gttcctggaa cgggcgcgtc tgaaaatcga gccgctgatc
      721 gaggctggaa ttcaattcgt ttatctcgaa gatttgggca aagaaatcag cggcgctcaa
      781 aaatttctgt cgctgttgcg catgactttc aatcccggtt cgttgactcc aaaagatgat
      841 tcacgtttgt cctccactcc gcaatcagcc gtgattcttt tcaccagcgg ttcggaaggt
      901 gttcccaaag gcgttgaact ttcacacaca aacttgttgg cgaacatgcg ccagatgctc
      961 gcggtgaccg acatcacgga cagggaccgg ttgttcaatt gtctgccgat gttccacagc
     1021 ttcggcctga cggtgggaac tctgctgcca ttggtgcgag gcatttacgt ctttgtctat
     1081 ccatctccgc tgcattatcg cgtggtgccg gctctttttt acgagaccga ctccagcatt
     1141 cttttgagca cgaacacatt cttgaacggc tacgcgcgca agggaaatcc ctacgatttt
     1201 cgctcgatgc gttatttgtt tgccgcagcg gaaaaactcc aggaagccac ggccagcacc
     1261 tgggcgcaaa agttcggcgt gcgcatcctt gagggctacg gcgcgacgga atgctcccca
     1321 tgcgtgagcg tgaacactcc gctttcgccg aaatacggct cggttggccg gctgttgccg
     1381 gggatggaat gcagattgga aaagatcgaa ggcgtggagg aaggcggtga attgtttgtg
     1441 cgcgggccga atgtgatgat aggttatttg aacgcggatg cgaacgcgaa attcaaggcg
     1501 ttgaacggct ggtacgacac cggcgacatc gtgagcgtgg atgaggaagg gtatctgtcc
     1561 attcgcgggc ggttgaaacg gttcgcaaaa gtcagcggcg agatggtgag tttgaccgcc
     1621 gtggaagacg cgttggccgg ggcatttccg caatatggcc tgcgttgcca ggttgccgtg
     1681 gtgacgcgcc ccgacgaagg caaaggcgaa gcgctcatcg ccgtgaccaa cgagccgaaa
     1741 ctgcaactgg aagaaatccg cgccgtgatc aaggcgaagg gcttgagcaa tttgagcgcg
     1801 ccgcgtgaaa tcaaggtggt gcgtgaaatc ccgaaacttg gcaccggcaa aatcaatcat
     1861 cgcgaacttg cgacgatgat caacaaatga cgacgttggc aaccatcatc agtttttgcg
     1921 tgggcctttt catctggctt atttcgcaaa acgtcggggc ggaaa
//
LOCUS       DASZMM010000256         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5315255, whole genome shotgun sequence.
ACCESSION   DASZMM010000256 DASZMM010000000
VERSION     DASZMM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5315255"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VG754_06175"
     CDS             <1..1067
                     /locus_tag="VG754_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18834.1"
                     /translation="KLFGYSAAEMIGDSIIRLIPQNLRQEEEHILQKISAGERVATFD
                     TERIHKAGHLIPVSVSVSPIKDDQGKIIGASKVARDITARKRSEQALRDSYESLERKV
                     AERTAELQSAKERAEAGARAKSQFMASMSHELRTPLNGIIGFSEFLVDGKPGALNEKQ
                     KEYLEDILNSGRHLLQLINDVLDLSKVEAGRIQFSPEKFQLRKAIDEVCAVAMPMGQK
                     KGIQVGLTMGNGLNGVMLDPVKFKQVVYNLVSNALKFTDAGGHVDIRCDALAGNKFSL
                     AVTDSGIGIKEEDLQRLFREFEQIDSGNQRRYEGTGLGLALTKKLVELQGGGVSVQSE
                     FGKGSTFTVILPVKHEDTKA"
     gene            1064..1459
                     /locus_tag="VG754_06180"
     CDS             1064..1459
                     /locus_tag="VG754_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18835.1"
                     /translation="MSARILIVDDNPVNLKLAANVLAFDGYTVLQAADAEEAMAIIAD
                     NKPDLILMDIAMPGMDGLTLTRKIKSDPATAKIIVVALTASAMKGDEDKAKAAGCDGY
                     ITKPVDTRTLSSKVAEALAPLLEKNERNA"
     gene            1456..1815
                     /locus_tag="VG754_06185"
     CDS             1456..1815
                     /locus_tag="VG754_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18836.1"
                     /translation="MNILVVEDNPSHLKLAHHVLSAAGNQVQSVEAAVEAFAAIKRDK
                     PEIILLDLELPGMDGLSLVRQLKADPETADIPVVAVTSYPERFSRADALAAGCVAYIT
                     KPINTRTLPDQLESLMK"
     gene            1847..2116
                     /locus_tag="VG754_06190"
     CDS             1847..2116
                     /locus_tag="VG754_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18837.1"
                     /translation="MKVLIVDDTPGNLRLLRAVLEGEKIDVVEAADGLQGLAVLEKEP
                     VDAVISDILMPNMDGYRFCREVRSNDRLSRLPFLIYSSTYNSPAD"
     gene            2126..>3319
                     /locus_tag="VG754_06195"
     CDS             2126..>3319
                     /locus_tag="VG754_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWF18838.1"
                     /translation="MSMGVDKFVKKPAPIQEIIRNLDEVIRRGPRPLEPEAHRELDLA
                     KLYNETLVNKLEQKNVELSEQAEALRASEEKFRQLAENINEFCWISNAETMETLYASP
                     AYEKIWGRSCQSLYDNALSFLEGVPEGDRAGIRAGMESMKRGEPMDLEHRVIHPDGSP
                     RWVHARGSGVRNEKGEIYRLVGICEDITRRRSTEDQLRQAQKMEAIGQLAGGVAHDFN
                     NLLTIIGANLDLVIMTEENLSPGAKDYLSHVSHAADRAATLTRQLLAFSRSEAMHVQV
                     VNLNELISTFTKLLGRILGEDVRLVSDFAPNMPPVKVDAGMIEQVLMNLSVNARDAMT
                     AGGRLTIRTELQTIDAAHVEANPRARSGRFACISVEDIGTGIAPEHMSRIFEPFFTTK
                     GVGKGT"
BASE COUNT          794 a          934 c          943 g          648 t
ORIGIN      
        1 aaaaactttt cggctattcg gcggcggaaa tgatcggcga ctccattatc cggctcatcc
       61 cccaaaatct ccggcaggaa gaagaacaca ttcttcaaaa aatcagcgcg ggcgagaggg
      121 ttgccacgtt cgataccgag cggattcata aagcgggtca tctcattccg gtttccgtat
      181 cggtttcccc catcaaagac gatcagggga aaatcattgg cgcgtcgaaa gtggcgcggg
      241 acatcaccgc acggaagcgg tctgaacaag ccttgcgtga ctcctacgaa agccttgagc
      301 gcaaggtggc cgaacgcacg gcagaattgc aatcagccaa ggaacgcgcc gaagccggag
      361 cccgggccaa atcccaattc atggcctcga tgagccacga attgcgcaca ccgctcaacg
      421 gcatcattgg cttttcagaa tttcttgttg acggaaagcc cggcgcgctc aatgaaaaac
      481 aaaaggagta tctggaggat attctcaaca gcggacgcca tctgttgcag ctcatcaacg
      541 atgttttgga tttgtccaag gtggaagccg ggcgcatcca atttagtccg gagaaatttc
      601 agcttcgcaa agccattgac gaggtctgcg ccgtcgccat gcccatgggg cagaaaaagg
      661 gcattcaagt cggcctgacc atgggaaacg gcctcaatgg cgtcatgttg gacccggtca
      721 aattcaagca ggtggtttac aatctcgtct ccaacgccct caaattcacc gacgccggcg
      781 gccatgtgga catccgttgt gatgcactag ccgggaataa attttcactg gcggtcaccg
      841 atagcggcat tggcatcaag gaagaggatt tgcaacggtt gtttcgggag tttgaacaaa
      901 ttgactcggg gaaccagcgg cgttacgagg gcaccggact gggtcttgcg ctgaccaaaa
      961 aactggtgga attgcaaggg ggcggggttt ctgttcaaag cgaatttggc aagggcagca
     1021 cgttcaccgt gattttgcct gtcaaacatg aggacacgaa ggcttgagcg cgcgcatcct
     1081 catcgtcgat gacaatccgg tgaacctcaa actggccgcg aatgtcctgg ctttcgatgg
     1141 ctacaccgtt cttcaggcgg ccgacgccga agaagcgatg gcgatcatcg cggacaacaa
     1201 gccggacttg atcctgatgg atattgccat gccgggcatg gacggattga cgttgacccg
     1261 gaaaatcaaa tccgatccgg ccacggcgaa aattattgtt gtcgcgctga cggcctccgc
     1321 catgaaaggc gacgaggaca aggccaaggc ggccggttgc gatggttaca tcaccaagcc
     1381 ggtggacact cgaacactct cctccaaggt cgccgaagcg ctggcgcctt tgctggaaaa
     1441 aaacgaaagg aacgcttgaa catcttagtc gtcgaagaca acccctccca cttgaaactg
     1501 gctcatcacg tgttgagcgc ggccggcaac caggtgcaat cggtggaagc ggcggtcgag
     1561 gcatttgcgg ccatcaaaag ggacaagccg gaaatcattc tgcttgactt ggaactacca
     1621 ggaatggacg gcctgagcct ggtccgccag ctcaaggcgg atccggaaac ggccgatatt
     1681 cctgttgtcg ccgtgacctc ctacccggag cgcttttcac gggccgacgc gctggcggcg
     1741 ggatgtgtgg cttatattac caaacccatc aatacgagga cactgccaga tcaattggaa
     1801 agcctgatga aatgacccgg cgcctcctgg cgccctcacg caaaatatga aagttcttat
     1861 tgtagatgac acgccgggca atctccggct cctgcgcgcc gtgctggaag gcgaaaaaat
     1921 tgatgtcgtt gaagccgccg atgggctcca gggcctcgct gtcctggaaa aagagccggt
     1981 ggatgccgtc atttccgata ttctcatgcc caacatggac ggctaccgct tctgccgcga
     2041 agtgcggagc aatgaccgcc tgagccgttt gccattcctg atttattcca gcacctacaa
     2101 ctcgcccgcg gactaaaagc tggccatgag catgggagtg gacaaatttg tcaagaagcc
     2161 cgctcccatc caggagatca ttcgcaacct tgatgaggtc attcgccgcg ggccccgccc
     2221 gctggagccg gaagcgcacc gcgaattgga cctggcaaaa ctttacaacg aaacccttgt
     2281 caacaagctg gaacagaaaa acgttgaact atccgagcag gccgaagccc tccgcgccag
     2341 cgaggaaaaa ttccgccaac tggccgagaa catcaacgag ttttgctgga tcagcaacgc
     2401 cgagacgatg gaaacgttat acgccagccc cgcctatgaa aaaatctggg gccggtcctg
     2461 ccagagtctt tacgacaacg cgctttcatt tcttgaagga gttcccgagg gcgaccgggc
     2521 cggcatccgc gccgggatgg agtcgatgaa acgcggcgag ccaatggatt tggaacaccg
     2581 cgtgattcat cccgacggtt cgccgcggtg ggttcatgcg cggggctccg gtgtgcgaaa
     2641 cgaaaagggg gagatttatc gcctggtcgg catctgcgaa gacatcaccc gccggcggag
     2701 caccgaggac cagcttcgcc aggcgcagaa aatggaggcc attgggcaac tcgccggcgg
     2761 tgtcgcccac gatttcaaca acctcctcac cattatcggg gcgaacctcg acctggtcat
     2821 catgaccgag gaaaatctca gccctggagc caaggactat ctcagccatg tttcccatgc
     2881 ggccgaccgc gctgccacgt tgactcgtca attgctggcc ttcagccgca gtgaagccat
     2941 gcacgtgcaa gtggtcaatt tgaacgagtt gatttccacc ttcaccaagt tgctgggccg
     3001 tattcttggc gaagacgtcc ggctggtcag cgattttgct ccgaatatgc ctccggtcaa
     3061 agttgatgcg ggcatgatcg agcaagtgct gatgaacctt tccgtcaatg cgcgcgacgc
     3121 gatgacggca ggcggacgat tgaccattcg cacggagctt cagacgattg atgccgccca
     3181 cgtcgaggcc aatccacgcg cgcgttcggg acggtttgcc tgcatcagtg tggaagacat
     3241 tggaaccggc atcgcacccg agcacatgtc tcgcattttc gaaccgttct ttaccaccaa
     3301 aggcgtgggc aagggaacc
//
LOCUS       DASZMM010000257         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4228211, whole genome shotgun sequence.
ACCESSION   DASZMM010000257 DASZMM010000000
VERSION     DASZMM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4228211"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VG754_06200"
     CDS             complement(<1..239)
                     /locus_tag="VG754_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18839.1"
                     /translation="MSDTGKLVEQVEQSIAQRRLFRRGESILVAVSGGLDSMVLLHLL
                     ARMAEEQGWRLTVAHFNHQLRGRSSDADERLVERTA"
     gene            complement(457..1803)
                     /locus_tag="VG754_06205"
     CDS             complement(457..1803)
                     /locus_tag="VG754_06205"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF18840.1"
                     /translation="MKTDMTLRTRREFLRTTVLGGALASTVPAFLANTFSALQASAAD
                     SAVQTTTGRDGDILVVLQMAGGNDGLNTVVPYSNDYYRRSRPRIGIAASDVHKLNDEL
                     GLHPSLAGFKDLYDNGQLSVVQGIGYPNPNRSHFRSTEIWQTASDSQKFEKYGWLGRY
                     FDNACAGCDPTVGVSIGRQMPQAFAAQHPLGISLENPQQYRFISGDPKHHDGSEESFR
                     KLNEADEAMMSSDSGGAEGNSGGTIGSIHGKTPHTGSVLDFVERTSMDAEMSSDKIIA
                     VSNKVQNKAQYPQSPLANNLKLVARLIGGGLPTRVYYVSQGGYDTHVNQLPAQQRLLK
                     DLGDSVKAFTEDMKAQGNLSRVLVMTFSEFGRRVAENANGGTDHGAAAPMFVIGDKVK
                     AGLLGKYPSLAPGDLFNGDVKFTVDFRTVYAGLLEGWLKTPSAPILGRQFQPLMCV"
     gene            complement(1822..2037)
                     /locus_tag="VG754_06210"
     CDS             complement(1822..2037)
                     /locus_tag="VG754_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18841.1"
                     /translation="MNDSNQPNQSTGEQNANIASDLKAVAVELFKKSQRLPPLSLILN
                     KGKTQRAKTQRLRMPPEGFEFADFYHL"
     gene            complement(2041..3534)
                     /locus_tag="VG754_06215"
     CDS             complement(2041..3534)
                     /locus_tag="VG754_06215"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HWF18842.1"
                     /translation="MLKALAKKNWNFTTAAHLLNRAGFGGTPKEIAALESMGLEQAVA
                     HLVDYEKIPDSTPDPDWAKPDPTRAERLRKARDADPEARRKMVQEEQRNQRQNLIQLK
                     HWWLERMASGPRPLQEKMTLFWHGHFATSAEKVRDAYLMWMQNDLFRKQATGFWLELL
                     IATGKDPAMLVWLDQAKSRKEHPNENFAREVMELFTLGEGHYTEKDITEGARALTGWT
                     YDGLDQEFIERPRFHDTGQKTFLGQTGNLNGDDVLRIIVAQPQAARFITGKIWNFFAG
                     EEPSEELTTALADVFRKAGSNFKPLLKAIFNSEEFYADNIIGNQVKSPVQWLVGSVRV
                     LERDLPGPFICFGLTKNLGQDLFQPPNVKGWDGGLSWITTNNLLARYNEAAILVQGDF
                     NLLRTTMFAAGVNPQVRNTNNMLVSNRLRNLRLETVDVEKILTVEERNDKGKLLAALE
                     KRLLQGKLRGKQEQALREYLDGQSSLDDGVILKAIRLMMSTPEYQLT"
     gene            complement(3568..3876)
                     /locus_tag="VG754_06220"
     CDS             complement(3568..3876)
                     /locus_tag="VG754_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18843.1"
                     /translation="MKKSLTMVLYAGLCVALTGCVCVPDRCHNDYVTYYAPSPPPAVW
                     YAQPTYSSHRAWYRVRHRLWDDGYSFSNDPANHDVLAPVLGLDIYFPPAPQPYVAIDQ
                     "
BASE COUNT          953 a         1195 c         1131 g         1002 t
ORIGIN      
        1 gccgtgcgtt ccaccaggcg ttcatcggca tcgctgctgc gcccgcgcaa ttggtgattg
       61 aaatgcgcca cggtcaagcg ccatccctgc tcttcggcca tccttgccag cagatgcagc
      121 aacaccatgg aatccaaacc gcccgaaacc gcgactaaaa tggattctcc gcgacggaac
      181 agccgccgtt gcgcaatgga ttgttcaact tgctcgacca atttacccgt atcgctcacg
      241 gcgacagggt acggccaaat tacagaattt cacaaggaag attgagacag aattaataga
      301 agaaacagaa tgaaaagcga gacacacaga tattaaacta cgctgtattt ggagtggcgc
      361 atcaaaacta tattgcgcga gggaacgcta aaatgccatc gaatgaacaa ccgatccctt
      421 ttcaaattct gtccattttg ttcattctgt caaaaatcac acgcacatca gcggctggaa
      481 ttgccgcccg agaatcggcg cgcttggtgt cttgagccag ccttcgagca ggcccgcata
      541 caccgtgcgg aaatcgacgg tgaatttcac atcgccattg aacaggtcgc cgggtgcgag
      601 gctcggatat tttccaagca ggccggcctt caccttgtca ccaatgacaa acattggcgc
      661 agccgcgccg tgatcggtgc caccgttggc gttttcagcg acgcgccggc cgaattcgct
      721 gaaggtcatc accagcacgc gcgacaggtt gccctgcgct ttcatatcct cggtaaacgc
      781 ctttaccgaa tcgcccaaat ctttcaacaa acgttgctgc gcgggaagct ggttgacgtg
      841 cgtgtcatat ccgccctgcg aaacataata gacgcgcgtc ggcaaaccgc cgccaatcaa
      901 ccgcgcgacg agcttcaaat tattggcgag tggcgattgc ggatattgcg ccttgttctg
      961 caccttgttg ctgacagcga tgattttgtc cgaactcatc tccgcgtcca tcgaggtccg
     1021 ttcgacaaaa tcgaggaccg aaccggtgtg cggggttttt ccatggatgg aaccgatggt
     1081 gccgcccgaa ttcccttccg cgccgcctga atcggatgac atcatcgctt cgtccgcttc
     1141 gttcaatttg cggaaagatt cttccgagcc gtcgtgatgt tttgggtcgc cagaaatgaa
     1201 acggtattgc tgcggatttt ccaggctgat gccgagcggg tgttgcgcgg cgaaagcctg
     1261 cggcatctgc cggccaatgc tcacgccgac ggtcggatcg caacccgcgc aggcattatc
     1321 gaagtaacgc ccaagccagc cgtatttctc gaacttttgc gaatcgctcg ccgtctgcca
     1381 aatttccgtg gagcgaaaat gcgaacggtt cgggttcgga tacccgatgc cctggacgac
     1441 cgaaagttgg ccgttgtcgt aaaggtcctt gaatccagcc aaactgggat gcaaaccgag
     1501 ttcgtcattg agcttgtgga cgtcagacgc agcgatgccg atgcgcggac ggctccggcg
     1561 ataataatcg ttgctgtacg gcacgactgt gttcaaccca tcattgccgc ccgccatttg
     1621 caggacgacc aggatgtcgc catcgcgtcc ggtagttgtc tggacggcgg aatccgcggc
     1681 gctggcttgc agcgcggaaa aagtgttggc gagaaaagcc gggactgttg aagcgagcgc
     1741 gccgcccaaa actgtggtgc gaagaaactc gcggcgggtg cgaagtgtca tgtcggtttt
     1801 cataaattgt gaataaaaaa tttagagatg gtaaaaatca gcgaactcaa aaccttcggg
     1861 aggcattcgc agccgttgtg tttttgcccg ctgcgtcttt cccttgttca ggataaggga
     1921 caatggcggc aggcgctgac ttttcttgaa cagttcgaca gccacggcct tgaggtcgct
     1981 ggcaatgttc gcgttttgtt cgccggtcga ttgatttggt tgatttgaat cgttcataac
     2041 tcaagtgagt tggtattccg gtgtgctcat catcagacgg atggccttta aaatgacccc
     2101 atcgtccaat gaactctggc cgtccaggta ttcgcgcaat gcctgctcct gcttgccccg
     2161 caatttcccc tgcaacaatc gtttctccag agcagccagc aacttgcctt tgtcgttgcg
     2221 ctcttccacc gtcaaaattt tttccacatc cactgtttca agccggagat ttctaagccg
     2281 gtttgagacc agcatattat ttgtattccg cacttgaggg ttgacccctg cggcaaacat
     2341 ggtcgtgcgc agcaagttga aatcgccctg gacaagaatc gccgcctcat tgtaacgggc
     2401 cagcaaattg ttggtagtga tccaactcaa gccgccgtcc caacctttga cgttgggcgg
     2461 ctggaacaaa tcctgtccca agtttttggt cagcccaaag cagatgaacg gtcccggcaa
     2521 atcgcgttcc agcactcgca cactgccgac gagccattgc accgggcttt tcacctggtt
     2581 gccgatgatg ttgtccgcgt aaaactcttc ggaattgaat atcgccttga gcagcggctt
     2641 gaaattgctg cctgccttgc ggaaaacgtc agccagcgcg gtcgtcagtt cttctgacgg
     2701 ctcttcgccc gcgaaaaagt tccagatttt acccgtgatg aaccgtgccg cctgcggttg
     2761 cgccacaatg atccgcagga catcgtcacc attcaaattg ccggtttgtc caagaaatgt
     2821 tttctgcccg gtgtcgtgga agcgtgggcg ctcgatgaat tcctggtcca gcccgtcgta
     2881 agtccagccg gtcaacgcgc gcgcgccttc cgtgatgtct ttctccgtgt aatggccttc
     2941 gccgagcgtg aacaattcca tcacttcccg cgcaaaattt tcgttcggat gctccttgcg
     3001 gctcttggcc tgatcgagcc agaccagcat ggcggggtcc ttgccagttg cgatgagcaa
     3061 ttcgagccaa aaaccggtcg cctgctttcg aaacaggtcg ttttgcatcc acatcaagta
     3121 ggcatcacgc actttttccg cgctcgtcgc aaaatgcccg tgccagaaca gcgtcatctt
     3181 ttcctgcaat gggcgggggc cgctggccat ccgctccagc caccaatgct tcaattggat
     3241 caggttctgg cgctgattgc gctgctcctc ctgtaccatc ttgcgacgtg cttcggggtc
     3301 cgcgtcgcgg gctttgcgca gccgttcagc gcgggtcggg tctggcttgg cccaatcggg
     3361 gtcgggagtc gaatcgggga ttttttcgta atctacaagg tgggcaaccg cctgctccaa
     3421 ccccatgctt tcaagggccg caatctcttt gggcgtgccg ccaaacccag cccggttcaa
     3481 gagatgggct gcggtggtga aattccaatt tttcttcgcc agcgccttca acatgattga
     3541 ttagacgcag gctccggcga aaaggtttca ctggtcaatg gcaacgtaag gctgtggcgc
     3601 gggcggaaaa tagatgtcca aacccaggac tggcgccagc acatcatggt tcgcagggtc
     3661 gtttgaaaac gagtagccgt cgtcccacaa acgatgccgc acgcgatacc aagcccggtg
     3721 ggacgaatag gtcggctgcg cataccatac cgcaggcggc ggactgggcg catagtatgt
     3781 cacataatca ttatgacaac ggtcagggac gcaaacgcaa cccgtgagag caacacataa
     3841 cccggcgtag aggaccatcg ttagagattt tttcatctga actccttggt caacaaaata
     3901 tccccgccgt cccgcatcgc aatggggtga aaaccgcaaa gaacctgtgc ctccagacgc
     3961 caagcgagtc gaggaccgcg attttattcg ccaaaccaga tgaatggcag aaaatgagat
     4021 tagatttgtt tattaggaaa tcctgaaatc aggaataaaa ttcttgagga aacatcgtct
     4081 tttcctgatt tcctgctttc ctaattctat tttcccacgg gacagctctg aaattttttt
     4141 atttttttgt agcatttcgc cgcgcgtttg ttacatctgc gcgcagtgca aataaaggca
     4201 gagtctgcgg ccgctcaaaa tcggatggcc gcaaaccagt cggccgggga tttattcgcc
     4261 atccaggccg aaaatttagt a
//
LOCUS       DASZMM010000258         4282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3407047, whole genome shotgun sequence.
ACCESSION   DASZMM010000258 DASZMM010000000
VERSION     DASZMM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3407047"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..462)
                     /locus_tag="VG754_06225"
     CDS             complement(94..462)
                     /locus_tag="VG754_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18844.1"
                     /translation="MKALIVALGLSGVLYAGCVSKSELKAKQRQAFLAGQKQGAVVAQ
                     ANANSVWIVGNVRNPSVPWTPELTLRQALIEADVQGAGDPSQIVVLRNGGQPTSVSAQ
                     ELLNGFDMPLQAGDRVEVRR"
     gene            complement(673..1809)
                     /locus_tag="VG754_06230"
     CDS             complement(673..1809)
                     /locus_tag="VG754_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWF18845.1"
                     /translation="MKFKLLNCFMLSAALLAIGCKNLRTAASVPAQSILQARDELRQR
                     DIPFDSDTFVRRAGDGDLESVELFLQAGMNPDTTNKFGYTGLMWASGQGREAVARALL
                     AKGANVAKQARDGSTALTAAASQGQIETTELLLSHGADINGGKDQGMTPLIAAARENR
                     TAMVERLLSKGADIDARDGDGYTALFYAAYNFGNEDANSATIPMVKLFLAHGAKVNAA
                     GKDGSTPLMWAAAKGEETIMEILMTAGADANAADTNGKTALLRAVEGQAGPSAVTVLV
                     SKGAQINAQDADGDTALIKASRRKRAGMSTALIARGARLNLQNKRGETALMAAAEYGN
                     RAIVKMLLQAGADPTLKNKKGQTALNIAEDEHTAAFERLKSGGE"
     gene            2233..3615
                     /locus_tag="VG754_06235"
     CDS             2233..3615
                     /locus_tag="VG754_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18846.1"
                     /translation="MTLQNVNSNAFGLLFERPVSGDLYPQPLIVSGLSIGGGTHNVVY
                     LATASNNVYCYDADVASNTAPFWSVNLGTPVPQADVDCSASDIATSVGIIGTPAIDTN
                     TLTMYVMAKNKNPDATYHQWLHALDITTGAEKFGGPVQIVATGFDPKLNNQRPGLLLQ
                     GGNVYIGWSSHNDCGGYHGIIAAYNASTLVQTAVWNDTPTGTKGGIWMSGGGLIGDGT
                     NIYLSIGNGDFNASSGGKNYGESIVGLNGSLNTVLTYFTPSSWRSLNGHDVDLGGCPV
                     LLIPGTRLLVTGGKDHNLYLVNADNMGGLGGGIQSFSDGSSENKGGLVAFNGPAGLLV
                     YIQPSGTSLEAYKMVNGVFNPTTPYWTSPIPPPTGLPGGQLWLSGTGTNAVLWETIPY
                     SANAEHSSVPGILRAFNPSTGAEIYDSHQNVTRDDFGNFAKNPSPVVANGKVYVPTFS
                     NHLAVYGLLQ"
     gene            3923..>4282
                     /locus_tag="VG754_06240"
     CDS             3923..>4282
                     /locus_tag="VG754_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18847.1"
                     /translation="MSRVRTQNKALQQKLRQFEELGRLRKDHDELFRMRNQIRELQAS
                     AEEKKSDLARTLSDLQAENLKLRDKYGQLEQTPEAISARLFVDANELTQIGQALRLYA
                     NMNSNRLPEDLAELKYYT"
BASE COUNT          990 a         1153 c         1081 g         1058 t
ORIGIN      
        1 aatttgaaat ttcaaatgaa aagcttcatc tcgaagagtt gaaaatcggt attcaagttt
       61 ggagattctc tggagcttgg aggttggggc ttttcaccgt ctcacttcca cgcgatcacc
      121 tgcttgcaac ggcatgtcga atccattcaa caattcttgg gcgctgacgg aagtcggctg
      181 gccgccattg cgcagcacca cgatctggct tggatctccc gcgccctgaa catccgcttc
      241 gatcaatgcc tgccgaagcg tcaattccgg cgtccacgga accgacggat tgcggacatt
      301 cccgacgatc caaaccgaat tcgcgttcgc ttgcgcgaca accgcgccct gtttttgacc
      361 cgccaaaaaa gcctgccgct gcttcgcctt aagctccgat ttggaaacac aacccgcgta
      421 caacacgccc gataaaccta atgcgacaat cagtgctttc atatctgcat attaacatat
      481 catgccacga atacttgcat ggttgcatcg tacaaagcct cattggcgga acaacgaaag
      541 ttgttggccg ttttcggtga attggaaata atggcctgag tattcacttc ggccgaccgt
      601 gtccccaggc agcgaggttt aaattgcaga aaacccgact cttcgctcgt gtatggaaaa
      661 tctgcggcgc aatcactcgc cgccggattt taatcgctcg aacgcagccg tgtgctcatc
      721 ttccgcgatg ttgagcgcag tctggccttt tttatttttg agcgtgggat cggcccccgc
      781 ctggagcaac atcttgacta tcgcccggtt cccatattcc gcggctgcca tgagtgccgt
      841 ctcacctcgc ttgttttgga gattcaatcg cgcgccacgc gcaatcaacg cagttgacat
      901 cccggcacgt ttgcgtcggg aggcttttat caacgctgtg tctccatcgg cgtcttgagc
      961 gttgatttgc gctcctttcg agaccaggac tgttaccgcc gaaggcccag cctgtccctc
     1021 gactgcgcgc aacaaagccg tctttccatt tgtatcagcg gcgttggcgt ccgctcccgc
     1081 ggtcatgaga atctccatga tggtttcttc gccttttgca gccgcccaca tgagtggagt
     1141 cgaaccatct ttgccggcag cattcacttt tgcgccatga gcgagaaaaa gtttcaccat
     1201 gggaatggtg gcgctgtttg cgtcttcatt tccaaaattg taagcggcgt aaaaaagggc
     1261 ggtatatcca tcaccatcgc gcgcgtcaat gtccgcgccc tttgacagga ggcgttcaac
     1321 catcgccgtt cgattttccc tggcagcggc aatcaggggt gtcatgcctt gatcttttcc
     1381 gccatttatg tccgccccat gactcaggag aagttcggtg gtttcaattt gtccttgaga
     1441 agctgctgca gtgagggcgg tcgaaccgtc gcgcgcctgt tttgcgacat tcgcgccttt
     1501 agccagcaac gctcgcgcta ccgcttcgcg gccttgacct gacgcccaca tcaacccggt
     1561 gtagccgaat ttattcgtcg tgtcgggatt catcccggct tggaggaata attcaacgga
     1621 ttcaagatcg ccgtctccag ctcttcgaac gaaggtgtcc gaatcaaatg ggatgtcgcg
     1681 ttggcgcaac tcatcccgag cctgcaaaat cgactgcgcg ggaacagacg cagcagtccg
     1741 caggtttttg cacccgatgg caagcagggc agcgctcaac atgaagcaat ttaataattt
     1801 gaatttcatg gtgatcaata aatgttggaa ctgtgttcgc tctcaaggct gcaaaaatct
     1861 ttcggattcg cggagagtgc tgggagcatt ccgtgcaggc taccaaaaaa gccaattatt
     1921 ttgcacccct ctttcgaggg ggttgtattt tttcggacag gtagtaatat tgcgccattc
     1981 tcatactcat tgcgtgtgca tagcttcatc gtgtgcatgt tttgtcaaaa actgtaaacc
     2041 atgaatgtcg agatctggca ttggactcca actttcgaca tcacgatacc aaaaggaaaa
     2101 aacatgagcc gagtaaaaat ctgttcgatc gggctgctgg caatattggc ctgtaacggt
     2161 cagtcagcgg taaccattcc gacgacaaaa ggtgataatg gacgaagtgg gcttacgccg
     2221 tcggaaacga ccttgactct ccaaaatgtc aattctaacg cattcggatt gctttttgaa
     2281 aggcccgtca gcggagacct gtatccgcaa ccattgattg tcagtggatt gagcattggt
     2341 ggaggaacac acaacgtcgt ttacctggcg acggcgtcca acaacgttta ttgttacgac
     2401 gctgatgttg cgagcaatac ggcccccttt tggtccgtga atctgggaac gccggttcct
     2461 caagctgatg tggattgttc ggcgtcggac attgcaacga gcgttggcat catcggcaca
     2521 ccggcgattg ataccaatac tctgaccatg tatgtcatgg ccaaaaacaa aaaccccgac
     2581 gccacctatc atcaatggtt gcatgctttg gacatcacaa ccggcgccga aaaattcggc
     2641 ggccccgtgc aaatcgttgc caccgggttt gatcccaaac taaacaacca acggccagga
     2701 ctcctgctgc agggtggcaa tgtgtatatc ggctggtcat cacacaatga ctgcggcggc
     2761 tatcacggga tcattgctgc ctacaatgcg agcacgcttg tccagacagc cgtctggaac
     2821 gacacgccaa ccggcacaaa aggcggcatt tggatgtcgg gcggcggact gatcggcgat
     2881 ggcacgaaca tttacttgag cattggcaat ggagatttca atgcctcaag tggtggtaag
     2941 aattacggcg aaagcatcgt tggtttgaat ggctcgctca acaccgtctt gacctatttc
     3001 accccctcaa gctggcgttc tttaaacggg catgacgttg accttggggg ctgtcccgtt
     3061 ttgcttattc cggggacccg gttgctggtg acgggtggca aggaccacaa tctttacctg
     3121 gtcaatgccg ataacatggg cggcttggga ggaggaattc aaagctttag tgacggcagc
     3181 agcgaaaaca aaggcggatt ggttgcgttt aacgggcctg ccgggttgtt ggtttatatc
     3241 cagccctcag gcacatcatt ggaagcctat aaaatggtca atggcgtctt caatcctacc
     3301 actccttatt ggacgagccc cattcctccg cccacgggat tgccaggcgg ccagctctgg
     3361 ttgtccggca ccgggaccaa cgccgtgttg tgggagacca ttccctatag cgcaaatgcc
     3421 gagcattcca gcgttccggg catccttcgc gcattcaacc caagcacggg tgcggaaata
     3481 tacgactctc atcaaaacgt gactcgcgat gatttcggca acttcgcaaa gaacccgtcg
     3541 ccggttgtgg ccaacgggaa ggtatatgta cccacgttct ccaatcacct tgcagtttac
     3601 ggtttgctcc agtgatggcc gtggcgaacg accctggacg gttcttgatt tgaagaaatc
     3661 tcagtggaat cgaggccagc ttttgcgcac agattcccga ttaaaagtca tccatccata
     3721 atgccagttg gcattatgga tggttctgtt ctaaattcac tgactggaga tattgcacgc
     3781 gtgatggaaa atgaatttgc gcttaacatt tatcggtctt ttcgttttgc ttttggtttc
     3841 cagcatttat tttttttccc tccattctca ttttcgtctt aacggaacaa acatcgccgt
     3901 cctgcaacgg caactggccg atttgagccg ggttcgcact caaaacaagg cgctccagca
     3961 aaaactccgg caattcgagg aactgggacg cttgcgcaag gaccacgacg agctttttcg
     4021 catgcgaaat cagatccggg agttgcaggc ttccgcggaa gagaagaagt ctgaccttgc
     4081 ccgcacttta tcagaccttc aggcagaaaa tcttaaactc cgcgacaagt atgggcaact
     4141 cgagcaaaca cctgaagcca tttcagcccg cctgtttgtg gatgcaaatg agctgacgca
     4201 aatcggtcag gctctccgtc tatatgcgaa tatgaacagc aaccggctgc ctgaagatct
     4261 cgccgaactt aaatattaca cc
//
LOCUS       DASZMM010000259        16051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1751960, whole genome shotgun sequence.
ACCESSION   DASZMM010000259 DASZMM010000000
VERSION     DASZMM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16051
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751960"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VG754_06245"
     CDS             <1..894
                     /locus_tag="VG754_06245"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HWF18848.1"
                     /translation="QENVQAAASAGYWPSFTGVSPAQTNGWLIFMDPFHIDSESWLNS
                     WNESYAPLPALGGLAAGPYNEQQTQVYLNGDVFEEGGVALSVGGGVSLAGIVSQGCMP
                     IGEPWTLTRVEGNVIHEIANRPAYEVLAETFNQLAVSDQKKARGNLFIGLVMDEYQED
                     FHQGDFLVRQLLVADPNSGAIAVGALPRTGQTVQFQRRDAAAANDDMLALLKQFAKNF
                     AGVTAYGGCLCCCNGRGRGMFREPDHDARLVQEHLGQMGIAGFFCNGEIGPVGQRNFL
                     HGYTASLALFVKKQQAPAAAH"
     gene            1237..2445
                     /locus_tag="VG754_06250"
     CDS             1237..2445
                     /locus_tag="VG754_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF18849.1"
                     /translation="MPSFVYVARETATGREVRNSVEAATEQAAITALLNRNLLVVSIQ
                     EKVGKKGKTSGGKVALQELVVFTRQLATMIDAGLAMVQSLQALVEQTTNKVMRDVIKD
                     ICSRVEAGDSFSEALVKHPKAFNKLFVCMVSAGEKGGLLAEILSRLATYLENTARLRR
                     KVKSSMMYPITVTVVAIGITIFLLVKVVPVFGDIFSSFGAKLPAPTQFLITLSNFVKK
                     FLILILFVMGGTVYGWLYFIKTKTGRAFWDSRRIKLPIFGSIAHKICLARFTRTFASL
                     VRSGVPILEVMNVVSNTCGNVVMENAIKTATVDIERGESISTALAKHPVFPSMIIRMM
                     NAGEQTGKIDSMLERIADFLDEEIETILSGLTALIEPLLIVFLGVVVGGIVIAMFLPI
                     FKMSEIINKH"
     gene            2792..6076
                     /locus_tag="VG754_06255"
     CDS             2792..6076
                     /locus_tag="VG754_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWF18850.1"
                     /translation="MARIILIAALLCQLAMVRDVTAGFLHTQGVTNVDINGNQIVLKG
                     VNLGSWLWPEFYMMGNPDLSAYANAGTGSGNINNYYDAFAAAIQDLMGGDTNLTAQVL
                     DAYWTNYITAPDIVYLRQQGFNSVRVPFDFEEFFQVTNWANNYPTNGYDINVGFKYLD
                     NLVGWCSSNGIYVIPDFHCPPGGPNNLSVVNYGGTTNTNSASVFTSAPNLALCGHIWA
                     RIAAHYATNPWIGGYDLLNEPVNTSGNNGQVGSPILANTFSNLVKVIRAVDTNHLLLC
                     EGDYYASTLYDVDDTRWSDPNRNLSFSDHDYGSSLPLGTGNKSTAVAANVPDWAGEFG
                     INSTCWYNHIVNLTYQLPTTLTSGGHTATIVEGNCFWAYKSCQYYTVVQNPQTPGWNR
                     LKAYWASGNSASLKPSVNDAYQWLIDYARAANFSNCVTHPEIVDSLMRAYTNAQLTGF
                     SQTALPYKTGARIPGKIFAVDYDMGDTNTAYVDTVSQDVANHGPGGTSWNNGSFGRDE
                     GVDTTKCADPGMLLKIGWNDAGEWQRHSLTCTPGNYNVYIRYAGGATGGQIDLSLLTI
                     SGAVVTSSNDVTGTLTLPATGSYTQYATYVLNNVTVTNSGPASLQVTVVNPGYDLLWV
                     EFAPSSGPPLPPTGETVVGAQAGVPAGLTGGVAAVAGNAVVSLNWVASEGATSYHVKR
                     ATNSSGPYSAIATAISLSYLDAGLTNGTTCYYAVAAVNNIGEGANSSFASATPQASTL
                     PSGWMDQDVGLATLWSGDAADVGWPGSASFNSGAFTVTGAGTDIWNTVDSFHYAYRGI
                     SGDCTNIVRVTSLQNTDPWAKAGIMLRESFNPDSVNALMTMTSQNGALFSSRTTTAAA
                     STSSGQTGVTVPYWLKLVRSGNNFTGYSSSNGVNWTQVGSATVPMATNVFVGLAVTAH
                     NNVLTNTAQFDSIGIIGSPPPPPASVSATLKNAHASLMWTPSPDAVSYNVKRATTNSG
                     PYQTIISNLVATNYVDPASMSGTTYYYVVSSVNENGEGPNSNPASVFVTLPSLAGILN
                     GQTLMLSWPASAPEFHLYSTSNLASPVMWMPVSNTVMSQGANLVVNAPLSGNASQYYR
                     LAMPQP"
     gene            complement(6077..8473)
                     /locus_tag="VG754_06260"
     CDS             complement(6077..8473)
                     /locus_tag="VG754_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18851.1"
                     /translation="MKSKKHSRERPAKIEPKSSRKIWAARCAIAVVIPLLVLGGLELA
                     LRMMGHGFPTSFFVRTQISGRDFFVTNEKFGYRFFPPALARTPLPLRMSAVKPTNEFR
                     IFLFGESAAQGDPDPSFGAWRYLEALLRERYPGTDFRVVCTAMTAINSHAILPIAREC
                     ARHDGDMWIIYMGNNELVGPFGASTAFGPRAPNRALVRASLALRKTKIAQWMDTLLAK
                     TSHGKNAPQSWSGLNMFKENKIHPDDPSWRRTCNNFSGNLEDIVSLGNASHVPVLLST
                     VASNLKDCAPFASLHPANFPGSQKTDWEHLYQDGTNAEYKGDFSGALAAYKNAMKIDP
                     AFAELWFRLGNCDLALTNFKDAKYEFERARDCDALVFRAESRINKIIKDTAARHADKN
                     VHLVDAAEMLAEESPNGITGQEIFYEHVHLNPAGNYLLARAFAGEIALMLPSSITAHA
                     KTNWLPADVCDQRLALSLWDRYRLWQMNFSRVSEPPFTEQLNDVPRAKKYMATMDEIK
                     QQLTQEAAEKAHVMYENAVAASPDDALLRQNYAQFLESMGSFPQAVAEQKRACECLPV
                     YPNLENKLGQLFIRDGKTAEAATCFSNALALNANFVPALNELGIVYANEGKVADAAAC
                     FARAKKINPGYLETYFNWGFLEQSQGHLDQALGYYQNAAQIQPNGPAAPFAQAVAAAV
                     AHNKGEAIRNFQTTLWMYPAFWQARYLLGVELATTENVADAQAQFLDVTRRRPDFARA
                     HLNLGVAFAKQGKLDDALAEFTVTLQLSPTNKAAQQFIETTRALKARKERSASAASPP
                     "
     gene            complement(8536..9165)
                     /locus_tag="VG754_06265"
     CDS             complement(8536..9165)
                     /locus_tag="VG754_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF18852.1"
                     /translation="MKNEDKIRLLVADDHALIRMGLVALVNTETDITVVAEAADGQQA
                     VDLFSKHNPDLVLMDLRMPVKNGIEATAEIRKKAPRARVLMLTTFDGDEDIHQALQAG
                     AQGYVLKGSTGQNLIPALRALAAGQRWVPPEVAKRLDARRGYEELTLREVEVLHELAK
                     GLANKEIADVLSISENTTKGHLKNIIAKLRVADRTEAVTAALQRGIIHL"
     gene            complement(9155..11404)
                     /locus_tag="VG754_06270"
     CDS             complement(9155..11404)
                     /locus_tag="VG754_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWF18853.1"
                     /translation="MTLRHLCAAPLLGMILLYLAATTLSAQEANVMTLTNASQVLSLP
                     ADRAILGENVRVVGVVTVFELNWDGRFFVQDETGGVFVDNPKGESPRTGDLVEVTGIT
                     HPGAFAPDITKPHWRKLGTAPLPVAKVVPIEQLMAGVEDSQRVEVVGIVREAAMEGDS
                     FRVTLANAGYRLRTYSPVLTNVDPLTLVGSQVRVRGTAAASYNAELRHLITVALYVPQ
                     AGDFVVERPALADPFESPILPLNNIAQYRKGAKPEERVHVKGVVTYQRLGEDLFIQDS
                     TGGLQVKSLELQQFAPGDVIEAVGFAGIQDYLPVLEDATFRKTSEPQMPVEANQVSLR
                     KLELGLHHADFVAMEGTLLDRNLRPFRQARTGKTWVQTSLLLQSSNLYFTGVAETPAA
                     DANLEAIPLGSRMEVRGICMTQSGEDGKFKSLQVLLPSPQDFRILARPSWLTPRRLGI
                     ALGILLAVVLVAVSWSIMISRKNSELNVQIREKEEAQAELQDAHDQLEMRVKERTEQL
                     KIQIAGRKESEIQFKGVLAERTRLAQELHDTLEQTLASIALQFDTCAKLFRKDADMAG
                     YHLELGRNILAQSQVDLRRSVWDLRSRALEQFSLRGALATSSKQITDGTGIKLQVHTT
                     GVVRPLPEIIEENLLRIAQEALTNTIKHSRAERAEMNLDYGPQSVSLQIADDGCGFAV
                     GDHAGPREGHFGLLGISERVQRIGGKLAINSNAGSGTVIRVEVPTETVLEIQWSSVGD
                     ANGELIDEK"
     gene            11616..15371
                     /locus_tag="VG754_06275"
     CDS             11616..15371
                     /locus_tag="VG754_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18854.1"
                     /translation="MSAMMVFLMLALCSASAQTLQFRFSFEDSGTSVTSGPDGALGSS
                     ITLNTLNFSGTAADFHGGANSGVQGAGQSLNFSSAVNAGTAANSTINGPIAQTLNNTA
                     FAALGTVSNFTATLWIKQFTSMTNTQSRGPRLMLFGPGVTDNNSTTNDISLYFQVTNT
                     LYFKINNTIISPPIYYNPLPTNVWLFIALAYDGTNGYFYMGSEATPAKLLTVSSNGGL
                     TVNFGASANLLLGNRSSDRARAFSGWFDEVRFYTGSGDANFVENIRQMSTPMVVSSIY
                     PDGLSLMEGTNQFSMAVASSNGIPTNGVTLFVNGTNVSSGLFFGGTSNNLTVSYTGLP
                     VNNVLPSTASLNAANISLQIVDSAGITTTNSITYDTFSTTNFTWEAEDYDFGGGVFID
                     NPVYAFTSQPNSYYQTSGNPGVDYSDNGAGPKSVYRSPFDLVETEYSVGNGLNGGTSV
                     GELMRQKIRDAYALDPTIRDVDMGHFDGPGTASGLPNWVNYTRTFPAGAFNVYTRVAF
                     GGTSGGAILSQVTNNWGTGSPGLLQLGTFSLPNTGGWQSYAWVPLRDSSGNLVRVDLP
                     AGTNTFQFTAGSGGGGNENFLMLSPANTNVPILSAIYPNGTNLMQFSTNLSFVASSPS
                     GVSIATNSIVVQLRGTNIFTSFATNITATNGLLVTGTATNLSVSCPLAPNTTYTAVIS
                     VTDANGNPAISSVSFDTYNPSYTWEAEDYNYSGGQAIYTAETNGYLGFGASDMIDTFA
                     PNFAANTGAQLYRSAGLNTEVNGDGPRLAYIGTGFTDYDIGYNNSGNWGNYSRSYPSG
                     VFNIVMRGANGSAAPGSATLALVSGDTTSTNQTTANIGTFTVPATGNWQGYTWVPLRD
                     NNGNLAQFNGIGSPLTLRVTSGSGYNANFYALLPANTNVPLLSNITPPGTNMFESTNT
                     LSFTASSSAGIAANSISVMLNGVDISSNITIGGTLTARTVTYPHLQPNTSYTVVINVR
                     DGNGNPAATTINFDTFSAANYTWEAEDFDHDGGQFTDNPQTNAYSGLGAVTDVDAHQV
                     NFAGTYLYRTNGCDTEVNGDQPRAQYQGTGFTDYTAGYFSDGAWMNYTRHYPTGTYNV
                     YGRMATGGGADSSCTLAEVTSGWGTTNQTLGSLGTFSVPFTAWESYTFVPLRDDSGNL
                     VSVTFNGSTNTLQVGRPTPITLLQDVNVNFLMLVPVFNIQATPNGANLNLSFATQSGF
                     KYQVQYKTNLTDVGWISLGSAVSGNGTVKSVSDSLAGTHRFYRVAIQ"
     gene            15450..>16051
                     /locus_tag="VG754_06280"
     CDS             15450..>16051
                     /locus_tag="VG754_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18855.1"
                     /translation="MKTSCCEVSGSKIREGFTLIELLVVIAIIAILASLLLPALTKAK
                     QKAQGIGCVSNLKQLQLAWVGYSGDNNDQIVRSGGQAFKVSYLPNAWTDPGNINNQWA
                     YGDITVPLASTNIVLLQVGLLYPYAGGIGIYKCPADKRTALWPSTSGPFTCRSMSMNA
                     WMNPIQSWNTTRAHSTLCVNFLKQSDVTQMGASTLFCFID"
BASE COUNT         3511 a         4605 c         4379 g         3556 t
ORIGIN      
        1 caggaaaatg ttcaagccgc cgcaagcgcc ggatattggc cgtcattcac gggagtcagc
       61 cccgcgcaaa caaatggctg gctgattttc atggacccat ttcatatcga cagcgaatcg
      121 tggctcaatt cgtggaacga atcttacgcg ccattgcccg cgctgggcgg actggcagcc
      181 ggcccgtaca atgaacaaca aacccaggtc tatttgaacg gcgatgtttt tgaagagggc
      241 ggggttgctc tttccgtcgg cggcggcgtc agtctggccg gaatcgtttc gcaaggttgc
      301 atgccgatcg gcgaaccatg gacgctgacg cgcgtggaag gaaacgtgat ccacgaaatc
      361 gcgaaccgtc ccgcttacga agtcctcgcc gagactttca accagcttgc cgtgagcgac
      421 caaaaaaagg cgcgcggcaa cctgttcatc ggtctggtca tggacgaata tcaggaggac
      481 tttcaccagg gcgattttct cgtccggcaa ttgctcgttg cggacccgaa ttcaggcgcg
      541 attgccgtgg gcgcgttgcc gcggaccggc cagacggtgc agtttcaaag gcgcgatgcc
      601 gcggccgcga acgacgacat gctcgccttg ctgaaacagt tcgcgaaaaa ttttgccggc
      661 gtgacggctt atggcggatg cctctgctgc tgcaatggac gcggacgcgg catgttccgc
      721 gaacccgacc acgatgcgcg cttggtgcag gaacatcttg ggcagatggg catcgcggga
      781 tttttctgca acggagaaat cggccctgtc ggccagcgca attttttgca cggctacacc
      841 gcttcgctcg cgctgttcgt gaaaaaacaa caggccccgg cagcggcgca ttgacgaccg
      901 ggcgttttgg aatgcttcga ctggactaac gaacgtctaa accttcccgg acccattgaa
      961 agacaatgct taaataataa ttagaaagtc gaatggaact ctgcactcct ttcttcaagc
     1021 catttgtccc aaaacgggac ggaatctcaa tttcacgcgg gcttttcaag caaaattttg
     1081 aagttttttt gccgttttcc gcgattccga gatgctggtt ggacgttttc ccatccatgg
     1141 aacggggttt gctttaactg cgagatgtct gctggaccgg tttggaattc cgaccgactg
     1201 ccagccattg ccgcaaaaag attagaccat atagatatgc cctcatttgt atatgtcgcc
     1261 agggaaaccg ccaccgggcg ggaggtacgc aactctgtgg aagccgccac ggaacaagcc
     1321 gccatcaccg cgctgttgaa ccggaacctg ctcgtcgttt ccattcagga aaaagtcggc
     1381 aagaagggca agacctccgg cgggaaggtc gcgcttcaag aactggtcgt tttcacccgc
     1441 caattggcaa ccatgattga tgccggcctt gcgatggtgc aatccctgca agcattggtc
     1501 gagcagacca ccaacaaggt gatgcgcgac gtcatcaagg acatctgctc gcgtgttgaa
     1561 gcgggcgaca gcttttccga agcgctcgtc aagcatccca aggcgttcaa caaacttttc
     1621 gtctgcatgg tgagcgccgg tgaaaaaggc ggtcttctcg ctgaaatcct gtcgcgcctt
     1681 gccacctatc tggaaaacac cgcccgtctg cgccgcaagg tcaagtcctc gatgatgtat
     1741 cccatcaccg tcacggttgt ggccatcggc atcaccatct tcctgctggt gaaagtggtg
     1801 cctgttttcg gcgacatctt ctcgagcttc ggcgccaaat tgccggcccc gactcaattc
     1861 ctgatcaccc tgagtaattt cgtcaaaaaa tttctcatcc tcatcctttt cgtgatgggc
     1921 ggaactgttt atgggtggct ctatttcatc aagaccaaaa cgggccgcgc attctgggat
     1981 tcgcgccgca tcaagctgcc catcttcggt tccattgcgc ataagatttg tctcgcgcgg
     2041 ttcacgcgga ctttcgcttc gctggtccgc agcggtgtgc ccatccttga agtcatgaac
     2101 gtcgtctcca acacctgcgg caatgtcgtg atggaaaacg ccatcaagac cgccacggtg
     2161 gacatcgagc gcggcgaaag catctccacc gcgttggcca agcaccccgt tttccccagc
     2221 atgatcatcc gcatgatgaa cgccggtgaa cagaccggca agatcgacag catgctcgaa
     2281 cgcatcgcgg acttcctcga cgaagaaatc gaaaccatcc tctccggcct cacggccctc
     2341 atcgaaccgt tgctcattgt gttcctgggc gtggtggtgg gcggcatcgt catagcgatg
     2401 ttcctgccca tcttcaagat gagcgagatc atcaacaagc actaacaccc cgtcgagccg
     2461 ctgcaaaacg cagggcttct cccatctcag cgttcaacat tcgatgttgg gcgttcgatg
     2521 tttcgtttat tctgggaaag cgcgggattt tctgaaattt ggcccaattc ggaaaacacc
     2581 gaacaaccaa cactgaacgc tgccccggag cgcggctcga tgagccgcag caacgcgcga
     2641 cgaacaggac gtaccagact aattcataca ctcttacttg ccacattgct gcgggtcgcc
     2701 gaccgcgctc cgggcaatgc gctgctttgc cttgcccccc ttttgagggg tgtaaagcaa
     2761 gcccgcaatc tctaaagtgg agggattcaa tatggcgcga attattttga ttgcggcttt
     2821 gctttgccag ttggccatgg tccgggacgt gaccgcaggc tttctgcata cccaaggcgt
     2881 gacgaatgtc gatatcaacg gcaatcagat cgtgcttaaa ggagtgaacc ttggaagctg
     2941 gctctggccg gaattttaca tgatgggaaa cccggatttg tccgcttacg ccaacgccgg
     3001 aaccgggagc ggcaatatca acaattacta cgatgccttc gcagcggcga tccaggactt
     3061 gatgggcgga gacaccaacc tgaccgcgca agtgctggat gcttattgga cgaattatat
     3121 aacggcgcct gacattgttt acctgcgcca acagggattc aacagcgtgc gcgtgccgtt
     3181 tgatttcgag gaattttttc aggtcaccaa ttgggccaat aattatccaa ccaacggtta
     3241 cgacatcaat gttggtttca aatatctcga caaccttgtg ggctggtgct caagcaatgg
     3301 catctacgtc atcccggatt ttcattgtcc gcccggcggg ccgaacaatc tttccgtggt
     3361 caattacggc ggaacgacga acaccaatag cgccagcgtt ttcacgagcg cgccgaatct
     3421 tgcgttgtgc gggcatattt gggcgcgcat cgcggcgcat tacgccacga acccatggat
     3481 cggcggatac gatttgttga acgaaccggt gaacaccagt ggaaacaacg ggcaggtggg
     3541 aagcccgatt ttggccaata ccttttccaa cctggtcaag gtcattcgcg cggtggacac
     3601 aaatcatttg ctcttgtgcg aaggcgatta ttacgcgagc acattgtatg acgttgatga
     3661 taccagatgg agcgacccaa acaggaacct ctcgttctcg gaccatgatt acggcagcag
     3721 cttgccgctc gggacgggca acaaatccac cgcggtcgcc gccaacgttc cggattgggc
     3781 gggagaattt ggcatcaatt cgacctgttg gtacaaccat atcgtcaacc tgacttatca
     3841 actgccaacc acgctgacgt ccggcgggca cacggcaacc atcgttgagg gcaattgttt
     3901 ttgggcatac aagtcctgcc aatattacac cgtggttcaa aatccacaga cgcccggctg
     3961 gaaccggctg aaggcgtatt gggcgtccgg gaacagcgcg tcgctcaaac cttccgtgaa
     4021 cgatgcgtat caatggttga ttgattacgc gcgcgcggcg aatttcagca attgcgtgac
     4081 gcatcctgaa atcgtggaca gcttgatgcg cgcgtacacc aacgcgcaac tcaccggttt
     4141 ttcgcaaacg gcccttccat acaaaaccgg cgcgcgcatt ccgggaaaga tttttgcggt
     4201 ggactatgac atgggagaca ccaacacggc ctatgtcgat accgtttcgc aggatgtggc
     4261 gaaccacggc cccggaggga cttcatggaa caacggctcc tttgggcgcg acgaaggcgt
     4321 tgacacaacc aaatgcgccg acccgggaat gttgttgaag atcggctgga atgacgcggg
     4381 cgaatggcag cgtcattctc tgacgtgtac gccgggaaat tataatgttt acatccgtta
     4441 tgccggtggc gcgacgggag gccaaattga tttgagcttg ctcacgataa gcggcgcggt
     4501 ggtcacctcg agcaatgacg tcaccggcac actcacattg cctgcaaccg ggagctacac
     4561 acaatatgcc acttatgttc taaacaatgt gacggtgacg aattcgggac ccgccagtct
     4621 gcaagtcacc gtagtgaatc ccggctacga tttgttgtgg gtggaatttg cgccgtcaag
     4681 cggacctccg ttgccaccca cgggagaaac agtcgtcggc gcgcaagcag gagttccggc
     4741 aggattgacc ggcggcgttg cggcagtggc gggcaatgcg gtggtttcgt tgaattgggt
     4801 cgcatcagaa ggcgcgacga gttaccacgt caagcgcgca accaacagca gcggtcctta
     4861 cagcgcgatc gccacggcga tctcgttgag ttatctggat gccgggttga cgaacggcac
     4921 gacttgctat tatgccgtcg cggcggtgaa caacattggc gagggggcaa attcaagttt
     4981 cgcaagcgca actccgcagg caagcacttt gccttcggga tggatggacc aagatgtggg
     5041 actggcaacg ctctggagtg gagatgccgc ggacgttggc tggccgggca gcgcaagttt
     5101 caacagcggc gctttcaccg tgacgggcgc gggcacggac atctggaaca ccgtggattc
     5161 atttcattac gcttatcgcg gcatcagcgg ggattgcaca aacatcgttc gagtgaccag
     5221 tttgcaaaat accgatccat gggcgaaggc gggaatcatg cttcgtgaat cgttcaatcc
     5281 ggattcggtg aacgcgttga tgaccatgac ttcgcaaaac ggcgcgttgt tctcgtcgcg
     5341 gacgacaact gccgccgcaa gcacgtcctc aggtcaaacc ggcgtcacag ttccgtactg
     5401 gctcaagctg gtgcgctcgg gaaataattt tacaggatac agttcgtcga acggcgtcaa
     5461 ttggacgcag gtaggctcgg ccactgtgcc aatggcgaca aacgtgttcg tcggcctcgc
     5521 agtgaccgcg cacaacaatg tcctcaccaa caccgcgcag ttcgattcca tcggcatcat
     5581 cggcagcccg ccgccgccgc ccgcttctgt gtccgcaaca ttgaagaacg cgcacgcgag
     5641 tctgatgtgg acgccatctc ctgatgccgt ctcgtataat gtgaaaagag cgacaacgaa
     5701 cagcggcccc tatcaaacca tcatcagcaa tttggtggcg acgaattacg tggatcccgc
     5761 ttcgatgtcc ggcacaacct attattatgt tgtgtcttca gttaatgaaa atggcgaagg
     5821 ccccaattca aaccctgctt cggtgttcgt caccctgccg agtttggcgg ggattttaaa
     5881 tggccagacg ctgatgttgt cgtggccggc gagcgccccg gaatttcatc tctactcgac
     5941 ttccaatctg gcgtcgcccg tgatgtggat gccggtcagc aacacggtca tgtcccaagg
     6001 cgcaaacctg gtcgtgaatg cgccgctctc cgggaacgcc agccagtatt atcggcttgc
     6061 gatgccgcaa ccttgatcag ggcggcgatg cggcgcttgc agaacgttcc tttcgcgctt
     6121 tcaacgcccg cgtggtttcg atgaattgct gcgcggcttt gttggtcggg cttaattgaa
     6181 gtgtgaccgt gaattccgca agcgcgtcat ccaacttccc ttgcttggca aaagccacgc
     6241 cgaggttgag atgcgcccgc gcaaagtcgg gccgtcgccg cgtcacgtcc aaaaactgcg
     6301 cctgcgcgtc ggcgacattt tcagtcgtcg ccaattcaac gccgagaaga tagcgcgcct
     6361 gccagaacgc gggatacatc cagagcgtcg tctgaaaatt cctgatggcc tcgcctttat
     6421 tgtgcgccac ggcggcggcc acggcctgcg cgaacggagc ggcggggccg ttgggctgga
     6481 tttgcgcggc attttggtag tagccgagcg cctggtccaa atgcccttgc gattgttcga
     6541 gaaagcccca attgaaatag gtttccagat aaccgggatt tatttttttc gcccgcgcaa
     6601 agcaggcggc cgcatcggca accttgcctt cgttcgcata aacaataccc agctcgttga
     6661 gcgcggggac gaaattcgcg ttcagcgcga gcgcattgga aaaacaggtt gcggcttcgg
     6721 cggtttttcc atcgcgaatg aataattgcc ccaatttgtt ttcgagattg ggatagaccg
     6781 ggaggcattc acaggcgcgt ttttgttccg caaccgcctg cggaaagctg cccatgcttt
     6841 ccaaaaattg cgcgtaattc tggcgcaaca aggcatcatc cggcgacgcc gcgaccgcgt
     6901 tttcatacat cacatgggct ttttcggcgg cttcctgcgt gagttgctgt ttgatttcat
     6961 ccatcgtcgc catgtatttt tttgcgcgcg ggacatcgtt caattgctcg gtgaacggcg
     7021 gctcggaaac gcggctgaaa ttcatctgcc atagtcggta tcggtcccac aacgagagcg
     7081 ccagccgctg atcgcaaaca tctgccggga gccagttcgt tttcgcatgg gcggtgatgg
     7141 acgatggaag catgagggca atctcaccgg cgaaagcccg cgcgaggaga taattcccgg
     7201 ctggattcaa atgcacgtgc tcatagaaaa tttcctggcc ggtgatgccg ttgggagatt
     7261 cttcggccag catttccgcg gcatcaacaa ggtgaacatt tttgtcggcg tggcgcgcgg
     7321 ctgtgtcttt gatgattttg ttgatgcggg attccgcccg aaagacaagc gcatcgcagt
     7381 ctcgcgcccg ttcgaattcg tatttggcat ccttaaaatt cgtgagggcc agatcgcaat
     7441 tgcccagccg aaaccaaagt tcggcaaagg ccgggtcaat tttcatcgcg ttcttgtatg
     7501 cggcgagcgc gcctgagaaa tcgcctttgt attccgcatt cgttccgtcc tgataaagat
     7561 gttcccaatc cgttttttga gatccgggga aattggcggg atgcaacgag gcgaaggggg
     7621 cgcagtcctt caagttgctg gccaccgtgc tgagaaggac gggcacgtgc gaagcatttc
     7681 cgaggctgac aatgtcctca aggtttccac tgaagttatt gcacgttcgg cgccagcttg
     7741 ggtcgtcggg atgaatttta ttttccttga acatgttcaa tccgctccag gattgaggcg
     7801 cattttttcc gtgggaggtt tttgcgagca acgtgtccat ccactgggcg attttggttt
     7861 tgcgcaaggc aagcgatgcg cgcaccaggg cgcgattcgg cgcgcgcggg ccaaaggcgg
     7921 tgctcgcgcc gaacggcccc accaattcat tattgcccat gtagatgatc cacatgtcgc
     7981 cgtcgtggcg ggcgcattcg cgggcgatgg gaagaatggc gtgggaatta atggcggtca
     8041 tcgcggtgca aaccacccgg aaatccgtgc ccgggtagcg ctcgcgcaac agcgcttcca
     8101 agtatcgcca cgcgccaaaa gaggggtcgg gatctccttg ggctgctgat tcaccaaaca
     8161 ggaaaatgcg aaattcattc gtcggcttca cggcggacat gcgcaacggc aggggagttc
     8221 gcgcaagcgc gggcggaaaa aagcgatagc cgaacttttc attggtgacg aaaaaatcgc
     8281 ggccggagat ttgcgtgcga acaaaaaagc tcgtcggaaa accatggccc atcatgcgaa
     8341 gggcaagttc caatcctccc aagaccaaca gcggaatcac aacggcaatg gcgcatcgcg
     8401 cggcccaaat tttgcgcgag gacttcggtt cgatttttgc gggccgttcc cttgaatgtt
     8461 tttttgactt caagcggtgt tctatcagag ggctaaattt ttttgccgcc attttgcaaa
     8521 tggcgatgcc tcacgctaaa gatggatgat gccgcgttgc aatgcggcgg tgacagcttc
     8581 agtccggtca gcgacgcgca atttcgcgat gatgtttttc agatggccct tcgtggtgtt
     8641 ttcgctgatg ctcaagacat cggcaatttc cttgttcgcg aggcccttgg ccaattcgtg
     8701 caacacttca acctcgcgca gggtgagttc ttcatagcca cggcgggcat cgaggcgttt
     8761 tgcgacttcc ggcggcaccc aacgctggcc ggcggcaagc gcgcgcaaag cgggaataag
     8821 attttggccg gtcgaacctt tcaacacgta accttgggcg ccggcttgaa gcgcctggtg
     8881 aatgtcctcg tcgccatcaa acgtggtgag catcaggacg cgggcgcgcg gagctttttt
     8941 gcggatttcc gcggtggctt cgatgccgtt ttttaccggc atccgcagat ccatcaacac
     9001 caggtcggga ttatgtttgg aaaagaggtc aactgcctgc tggccgtcgg cagcctcggc
     9061 gacaaccgtg atgtccgtct ccgtattgac gagagcaacc agtcccatcc ggatcaacgc
     9121 atggtcgtca gcgactaaaa ggcggatttt atcctcattt ttcatcaatc aattcgccgt
     9181 tcgcatcgcc aaccgaggac cattggattt ccaggacggt ttcggtcggc acttcaacgc
     9241 gaatgaccgt gcccgaaccc gcgttgctgt tgatggccag tttcccgcca atccgctgca
     9301 cgcgctcgga gatgcccagc agtccgaaat ggccttcgcg cggcccggca tgatcaccca
     9361 ccgcaaaacc gcaaccgtca tcggcgatct gcaacgagac ggattgcggc ccgtaatcca
     9421 aattcatttc agcgcgttcc gccctggagt gtttgatggt gttcgtcagc gcttcctgcg
     9481 cgatgcgcag caaattttcc tcgatgattt ccggcagtgg ccgcacaacg ccagtcgtgt
     9541 ggacctgcag tttgattccg gtgccgtcgg tgatttgttt gctgctcgtg gccaacgcgc
     9601 cgcgcaaact gaattgctcg agcgcccgcg agcgaaggtc ccagactgaa cgccgcaaat
     9661 ccacctggct ttgggccaaa atgttgcggc caagttccag atgataaccc gccatgtcag
     9721 cgtctttgcg gaacaatttc gcgcaagtat caaattgcag cgcgatgctt gccagcgttt
     9781 gttcaagagt gtcgtgcaat tcttgtgcaa gccgcgttcg ctctgccagg acgcccttga
     9841 attgaatttc ggattctttg cgtccggcga tctgtatctt aagctgttcg gtcctttcct
     9901 tcacgcgcat ctcgagctga tcatgcgcgt cttgcaattc agcctgcgcc tcctcctttt
     9961 cacggatctg cacgttcaat tcggaattct ttcgcgaaat catgatgctc cagctcacgg
    10021 cgacgagcac aaccgccagc aggattccaa gcgcgatgcc cagccggcgc ggcgtgagcc
    10081 agcttggccg cgcaagaatg cggaagtcct gcgggctcgg cagcaaaacc tgaagcgatt
    10141 tgaattttcc atcttcaccg ctttgcgtca tgcaaatccc gcgcacttcc atgcggctgc
    10201 cgagcgggat ggcttcgagg ttcgcatccg cagccggtgt ttccgcaacc ccggtgaaat
    10261 ataaattcga gctttgcaac aagaggcttg tctggaccca tgttttgccg gtcctggcct
    10321 ggcgaaatgg ccgcaggttg cggtccagca aagttccttc catggcgacg aagtccgcgt
    10381 ggtgcaagcc gagctcaagt ttgcgaagag acacctggtt cgcttcgaca ggcatttgcg
    10441 gttcagaagt tttgcggaac gtggcgtctt caagcactgg caggtaatcc tggattcccg
    10501 caaagccgac cgcttcaatc acatcgcccg gggcgaattg ctgcaactca agactcttga
    10561 cctgcaatcc tcccgtggaa tcctggatga acaaatcttc gcccagccgt tgatacgtga
    10621 cgacgccttt gacatggacc cgttcctccg gcttggcacc cttgcggtat tgcgcgatgt
    10681 tgttcagggg aagaatcggg ctttcaaagg gatcggccaa tgccggacgc tccacaacaa
    10741 agtcccccgc ctgcgggaca taaagcgcca cagtgatcaa atgccttagc tcggcattat
    10801 aggaagcggc cgcagtccct cgcacgcgga cctgagaacc gacaagagtc agcggatcca
    10861 cattggtaag gaccggcgaa taagtcctca gccggtaacc cgcgtttgcc agcgtcacgc
    10921 gaaaactgtc cccctccatg gccgcttcgc gcacaattcc gacaacttca acccgctggc
    10981 tgtcttcaac ccccgccatc aactgctcga tcggcacaac cttcgcgaca ggcaatggcg
    11041 ccgtgcctag cttgcgccaa tgcggtttcg tgatgtccgg cgcgaacgcg cccggatgag
    11101 tgattccagt aacttcgacc agatcgccag tccggggtga ttcgcctttc ggattgtcaa
    11161 cgaagactcc gcccgtctcg tcctgcacga aaaatcgtcc gtcccaattc agctcaaaga
    11221 cagtcacaac cccgacgacg cggacgtttt ctccaaggat tgcgcgatca gccggaagcg
    11281 aaagcacctg gctggcgttg gtcaaagtca ttacattggc ctcctgcgca gaaagggttg
    11341 ttgccgccag atacagaaga atcatcccca gcaacggcgc tgcgcaaagg tggcgcaaag
    11401 tcatgtttct acaggctttg ccgcgcgggg tcataggctg actctaaatc atttcgcttc
    11461 ggacagtcta gaaatttatc tgcgcggtac cacccgaaag aggggtgacg cgcgtttgat
    11521 gaatgattta agattcgagt atgacaccac gtactatgac tagcaagtcc atcccctcat
    11581 atcatttgcg tcattgcaaa atctgcctga aaagcgtgag cgcaatgatg gttttcctga
    11641 tgttggcgtt gtgttccgcg tcagcccaaa cacttcaatt ccggttctcc ttcgaggaca
    11701 gcggcaccag cgtgaccagc gggcctgatg gcgcgttggg cagcagcatc acgctcaaca
    11761 cgttgaattt ttccggcact gcggcggact ttcatggcgg agcgaattcc ggcgtgcaag
    11821 gcgcgggtca atcgttgaac ttttcttccg cagtgaacgc cggcacggcc gccaactcga
    11881 ccatcaacgg gcccattgcc cagaccttga acaacacggc attcgcggca ttggggaccg
    11941 tgagcaattt caccgcgaca ttatggatca agcaattcac ctcgatgacc aatacacaga
    12001 gccgcggacc ccggcttatg ttgttcggcc ccggtgtgac tgacaacaac tccaccacga
    12061 acgacatctc gctttatttc caggtcacca acacattgta tttcaagatt aacaatacca
    12121 tcatctcccc cccgatttac tacaatccgt tgccgaccaa tgtctggtta ttcattgcat
    12181 tggcttatga cggcacgaac ggctactttt acatgggtag cgaagccact ccggccaagt
    12241 tgctgaccgt ttccagcaat ggcgggctga ccgtgaattt cggcgcctcg gctaacctgt
    12301 tgcttggaaa tcgttcctcg gaccgcgcgc gcgccttttc gggctggttc gatgaagtcc
    12361 gcttttatac cggcagtggc gacgccaact tcgtggaaaa catccgccag atgtccacgc
    12421 cgatggtggt atcaagcatc tatcccgatg gcttgtcact gatggaaggg accaatcaat
    12481 tcagcatggc agtagcttca agcaacggga tacccaccaa tggagttaca ttgtttgtca
    12541 atgggacaaa cgtgtcatcc ggtctcttct ttggcggcac ttcaaacaat ctgacggtca
    12601 gttacaccgg ccttccggtc aataatgttc tcccctcgac cgcctccctc aacgcagcca
    12661 acatcagcct gcaaatcgtg gacagcgccg gcatcaccac cacgaacagc atcacctacg
    12721 atacgttcag caccaccaat ttcacatggg aagcggagga ttatgatttc ggcggaggcg
    12781 ttttcattga caaccccgtc tatgctttta ccagccaacc caacagttac tatcagacct
    12841 cgggaaaccc gggagtggat tacagcgata atggcgcagg cccgaagtca gtctatcgca
    12901 gtccatttga cctggttgaa acagaataca gcgtcgggaa tggtctcaac ggcggcactt
    12961 ccgtgggaga attaatgcgg caaaagatcc gggatgccta cgcgctggat cccaccatcc
    13021 gcgatgtgga catgggacat tttgacggtc ccggcacggc aagcggcctg ccgaactggg
    13081 tcaattatac gcgcacattc cccgccggtg cgttcaacgt ctatacgcgc gtggccttcg
    13141 gcgggaccag cggcggcgca atcctttccc aagtgaccaa taactggggc acgggttcgc
    13201 ccgggctgct gcagttgggc acattcagcc tgcccaacac cggcggatgg caatcctacg
    13261 cgtgggtgcc gttgcgcgac agttccggca acctggtgcg agtggacctg cccgcaggca
    13321 cgaacacttt tcaattcaca gccggaagcg gtggtggcgg caacgaaaat ttcctgatgc
    13381 tttcacccgc gaacaccaac gtgcccatac tttccgccat ctatccgaac ggcaccaacc
    13441 tgatgcagtt ttccacgaat ctttcttttg tggcttcgtc gccttccggg gtttccatcg
    13501 ccacgaacag cattgttgtg caactgcgcg ggaccaatat cttcaccagt ttcgcgacca
    13561 acatcacggc caccaacggc cttctcgtca ccgggaccgc cacaaatctt tctgtgagct
    13621 gtccgctcgc tcccaacacg acttacacgg cagtgatcag cgtcacggac gccaacggca
    13681 atccagccat ttcctccgtg agctttgata cttacaaccc atcctatact tgggaagcgg
    13741 aggactataa ttacagtggc ggacaagcga tctacaccgc cgagacgaac gggtatctag
    13801 gctttggagc ctctgacatg atagacacgt ttgcgccaaa ctttgccgca aatacaggcg
    13861 cgcaacttta tcgttccgcc gggttgaaca cggaagtcaa tggcgatggt ccaaggctgg
    13921 cgtatatcgg caccggtttc acggactatg atattggtta caacaattcg ggaaattggg
    13981 gcaactactc gagatcgtat ccttcgggtg tcttcaacat tgtgatgcgc ggggccaatg
    14041 gcagcgccgc gcccggcagc gccacccttg cgcttgtatc cggagacacc acctccacca
    14101 atcaaaccac cgccaacatc ggcaccttca ccgtgcccgc caccggcaac tggcagggat
    14161 atacctgggt tccgctgcgc gacaacaacg gcaacctggc ccaattcaac ggcatcgggt
    14221 ccccgttgac gctgcgcgtg accagcggct ctggctataa tgccaatttc tatgccttgt
    14281 tgccggccaa caccaatgtc ccgctcctct caaacatcac gccgccgggg acgaacatgt
    14341 ttgaatccac caacaccttg agtttcaccg cgtcttccag cgctggaatc gccgcgaaca
    14401 gcatttctgt tatgctcaac ggtgttgaca tctcttccaa catcaccatc ggcggcacct
    14461 tgaccgcccg caccgtcacc tatccgcatt tgcagccgaa caccagttat accgttgtca
    14521 tcaacgtgcg ggacggaaat ggaaatccgg ccgcgaccac gatcaatttc gacacgttca
    14581 gcgcggcaaa ttacacctgg gaagctgagg attttgatca tgatggcggt caattcaccg
    14641 ataatccgca aaccaatgcc tattcaggcc tcggagccgt gacggatgtt gacgcccatc
    14701 aggtgaactt tgcgggcact tatctatatc gcaccaatgg ctgcgacacc gaagtgaacg
    14761 gcgaccagcc tcgcgcgcaa tatcaaggca ccgggttcac ggattatacc gcgggttact
    14821 tcagcgacgg cgcatggatg aattacacgc ggcattatcc cacgggcacc tataatgttt
    14881 atggacgcat ggcgacgggc ggcggagcgg acagcagttg cacgctggct gaagtcactt
    14941 cgggttgggg aacgaccaat caaacactgg gatctcttgg gaccttctcc gttccgttca
    15001 cggcgtggga gagctacacc tttgtgccgt tgcgggatga ttccggcaac ctggtctccg
    15061 tcactttcaa cggttcgacc aacacgcttc aggtgggccg gcccacgcca ataactctct
    15121 tgcaggatgt aaatgtgaac ttcctgatgt tggtgccggt attcaacatc caggcgactc
    15181 ccaacggcgc caacttgaac ctctcgttcg cgacgcaatc cggattcaaa tatcaggtcc
    15241 aatacaagac caacctgact gatgtcggtt ggatttcact tggaagcgcc gtatcgggga
    15301 atggaacggt caagtcggtt tcggattccc tggctggcac tcaccggttt taccgggtgg
    15361 caatccagtg atctttggtg ggaaagactt ccggcagccg tcaggggtgg cggctgccgg
    15421 aagataattt aatcatgatt aaaagacgta tgaaaacgag ctgctgcgaa gtgagtggtt
    15481 ccaaaatccg cgagggcttt acgctcattg aacttttggt ggtgatagcc atcatcgcca
    15541 ttcttgccag cctgctcttg ccggcgctta caaaggcaaa acaaaaggcc cagggcatcg
    15601 gctgcgtcag caacttgaaa cagttgcaac tggcgtgggt tggctattcc ggggacaata
    15661 atgaccaaat tgttcgctcc ggcgggcagg cattcaaggt ctcatatctg cccaatgcct
    15721 ggactgaccc cggcaacatt aataatcagt gggcctatgg cgatataaca gttccactcg
    15781 cctcgaccaa cattgtcctg ctccaggtcg gcctgctgta tccctacgcg ggcggcatcg
    15841 ggatttacaa gtgcccggcg gacaaacgga ctgccttgtg gccctccact tcagggccgt
    15901 tcacctgccg cagcatgtcc atgaacgcct ggatgaatcc catccaaagc tggaacacca
    15961 cgcgggcgca ctccacgcta tgcgtgaatt ttctaaaaca atccgatgtg acccagatgg
    16021 gggcatccac gttgttttgc ttcatcgacg a
//
LOCUS       DASZMM010000260         4755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4774960, whole genome shotgun sequence.
ACCESSION   DASZMM010000260 DASZMM010000000
VERSION     DASZMM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4774960"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VG754_06285"
     CDS             <1..262
                     /locus_tag="VG754_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18856.1"
                     /translation="AVQKEVNYFHEHQNRMDYRAGRRRGEPIGSGAIESTCRQTQCRF
                     KRPGQYWSQQGDEALLCLETFWRNGRWHLLFPHHRRIDPSKN"
     gene            complement(637..2076)
                     /locus_tag="VG754_06290"
     CDS             complement(637..2076)
                     /locus_tag="VG754_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWF18857.1"
                     /translation="MKLTRWWWLSLLPMSWLAGCAVGPDYSRPSAPVPPAYSGETNEW
                     KVAEPRAHLPRGNWWEIFGDAELNRLEVEANAANQNLKASIAIFEQARAFADVSRANF
                     YPNVSANPSITRERDSANRPVNGVSSGIPQTYNTFTVPLDASYELDVWGRVRRTVESA
                     RANAEASRDDVETVRLSIQAEVASDYFTLHALDAEIALLRSSVTVFQKSLDLTRNRRA
                     GGIATDLDVAQAETVLKTTEALLPVTILERVQVEHALATLTGQPASIFKVAQRSLELE
                     PPILPAGIPSELLERRPDIAASERRMASANANIGVAKAAFYPKIELNGAAGFESVSAG
                     SVFSGSSRLWSLGPTVSFPLFNGGQLRAELREANAAYDQVVAQYRQSVLSAFEEVENN
                     LAAQQLLVAEDEAQAAALQAAQRTLNIANNRYQAGLVTYLEVATAENSALDLERTSVR
                     LRGDQLVTTVALIKSLGGGWQTPEKEASR"
     gene            complement(2073..3293)
                     /locus_tag="VG754_06295"
     CDS             complement(2073..3293)
                     /locus_tag="VG754_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF18858.1"
                     /translation="MNTLKNNKTSSLAEAPLPARSEGLAGNGANTSPPKLGRLMLLVV
                     LVLLAALIAGLVPRLMHRSELAKETVELSTPTVQVISAAPGKATPGLILPAEVRPFVD
                     APIYARASGFLKRWYVDIGGRVEAGQSLADIDTPELEQQLSGAKAELAQAEAASSLAK
                     ITADRWAGLLKTASVSEQENAEKQADLKLKLAAVDTANANVRRFEDLQSFTHVTAPFN
                     GTLTTRAVDVGDLISSGKELFRLSDTTKLRVFVRVPQTATPSIATGVDATLMVPELPS
                     RKFAAKVVRTSGAIDSNSRTLLTELEVDNSKGEILSGSYAQVSFADVRQDPALVLPAT
                     TLLFRDGLQVALVGTDGKVEIRNIDVGRDFGKTVEVVSGITSADKIINNPSDSLTTGT
                     TVRVAQAPLAQNNP"
     gene            complement(3280..>4755)
                     /locus_tag="VG754_06300"
     CDS             complement(3280..>4755)
                     /locus_tag="VG754_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF18859.1"
                     /translation="GMRVEETAKLVADVEDFIRREIPADELEGILDNIGIPNSSIALS
                     YSNNGLIGASDSDILISLKEGHHHPTEGYTRKLRRDLNRDFPSTMFYFLPADIVSQTL
                     SFGIPAPLNVQLTGWDRTKNREIAAKLADQMRNIPGAVDIRIQQPGDLPKLRFNIDRT
                     KSAAIGLSERDVANQVLLSVSGSGQVTPAYWLNTQYGIQYLINARAPERVLDSVAALK
                     SLPVSGGGAGQGNGQVLANVSTMTRIAGSPVFSHYNAVPVIDIFGNVSGRDLGGVLGD
                     LQRILKDTQKNLPRGTEIKLRGAAETMHSSFVGLGVGMVGAVLLVYFLLVVNFQSWLD
                     AFIIIMALPGALAGVIWALYLTFTTLSVPALMGAIMSLGVATSNAVLVVTFARLNMQQ
                     GMPPLKAALTAGAGRLRPVLMTALAMVIGMLPLSLGLGEGGEQNAPLGRAVIGGLFVA
                     TIATLFFVPVVFTIMHRNTAPGMAPAADLETNILHSTHEHA"
BASE COUNT          985 a         1407 c         1384 g          979 t
ORIGIN      
        1 tgcggtgcaa aaagaggtca attactttca cgaacaccag aaccggatgg attaccgggc
       61 cggacggcga cgaggcgaac ccatcggcag tggagctata gaatcgacct gtcgacaaac
      121 gcaatgtcgt ttcaagcggc cggggcaata ctggagccaa caaggcgatg aggcgctgct
      181 atgcttggaa accttctggc gcaatggccg ctggcacctt ctctttcccc atcatcggcg
      241 catcgacccc tctaaaaact gagatgcgcc ccccggatgt cttatgcaaa gtttttcatt
      301 gacaaaaaat aaaaatgaaa tcaccctacc gcatcgcgcg ttatttcgat gcggtagggt
      361 gccaatttaa agtggataga gaaaaactgt ggctgaattt gctgaggttg agtcaatgga
      421 ggtagggcgg agcagcggct acgcctttaa ttaaacggcc ttaaattggt ttccaggagt
      481 attctgtggt ctgttcggtt tatatttcca agagttcgct ttcgaagtta gaactttttg
      541 aaaaatcgta ttccgcagca agggtcgaaa gacgtgttca aatcctcctc gtcatcttgg
      601 cgctgtggaa ctctttaaac cgtccacttc gaggaactac cgcgaagctt ctttttcagg
      661 cgtctgccat ccaccgccca acgacttgat caacgcgacc gttgtcacca actggtcgcc
      721 gcgcaatcgg accgatgtgc gttccaaatc caacgctgaa ttctccgccg tcgccacttc
      781 aagataggtc acaagcccgg cttgataacg gttgttggcg atgttgagcg tccgctgcgc
      841 ggcttgcaac gccgcggcct gggcctcgtc ctcggcgacc aaaagttgct gcgccgccag
      901 gttgttctcc acttcctcga aggcgctcaa cacgctctgc cggtattgcg cgacgacctg
      961 gtcgtaagcg gcgttggcct cgcgcaactc cgcgcgcaat tgcccgccat tgaaaagtgg
     1021 aaagctgacg gtcggcccca gcgaccacaa ccggcttgac cctgaaaaaa ccgagcctgc
     1081 gctcacgctt tcaaatcccg ccgcgccgtt caattcgatc ttgggataaa acgccgcttt
     1141 cgcaacgccg atgttcgcat tcgccgatgc catgcgtcgt tccgacgccg cgatgtccgg
     1201 gcggcgttcg agcaattcgg acggtatccc cgccggcaaa atgggaggct ccaattcaag
     1261 ggagcgttgc gccaccttga aaatcgaggc cggttgtcca gtgagcgttg ccaaagcgtg
     1321 ctcgacttgc acacgctcca aaatggtcac gggcaacagc gcttcggtgg tcttcaacac
     1381 cgtttccgcc tgcgcgacgt cgaggtcggt ggcgatgccg cccgcgcggc gattgcgggt
     1441 caaatcaagg gacttctgaa aaaccgtgac gctggagcgc agcaacgcaa tttctgcatc
     1501 gagcgcgtga agcgtgaaat aatccgaagc gacctcggcc tggatcgaga gccgcacggt
     1561 ttcgacatcg tcccggctgg cttcggcatt ggcgcgcgcg gattcaacgg tccgtctcac
     1621 gcgcccccaa acatccagtt catagctcgc gtccagcggc acagtgaagg tgttgtaggt
     1681 ctggggaatg cccgaggaaa cgccgttcac tggccgattg gccgaatccc gttcgcgcgt
     1741 gatggacgga ttggcgctga cattcggata aaaattcgcg cggctcacgt ccgcaaacgc
     1801 gcgggcctgt tcgaagatgg caatggacgc ctttaaattt tggttcgcgg cattcgcttc
     1861 gacctcaagc cggttcaact cggcgtcgcc aaaaatttcc caccaattcc ctcgcggcag
     1921 atgcgcgcgc ggttcggcaa ctttccattc gttggtttcg cccgaatacg cgggcgggac
     1981 gggagcggaa ggacgcgagt aatccggccc cacggcgcac ccggccagcc atgacatcgg
     2041 cagcaatgaa agccaccacc agcgggtgag cttcatgggt tgttctgcgc cagcggcgct
     2101 tgcgcgacac gcaccgtcgt gccggtggtc agcgaatcgg aaggattgtt gatgatcttg
     2161 tccgccgagg tgatgccgga caccacttcg accgttttgc caaaatcgcg gcccacatca
     2221 atgttgcgta tctcgacttt cccatcggtt ccaaccagcg cgacttgcaa tccgtcacga
     2281 aacagcaacg tggtggcagg caacacgagc gcggggtctt gccgcacatc ggcaaaactc
     2341 acctgggcat aactgccgga aaggatttcg cccttcgaat tgtccacttc gagttcggtc
     2401 agcaacgtgc gcgaattgga gtcaatcgcg cccgatgtgc gcacgacttt ggcggcaaat
     2461 ttgcgcgaag ggagttcggg aaccatcagc gtggcgtcca cgcccgtggc gatgctcggc
     2521 gtggccgttt gcgggacgcg cacgaacacg cgtagtttgg tggtgtcgga aaggcggaac
     2581 agttccttgc cggaactgat gaggtcgccg acatcaaccg cccgcgtggt gagcgtgccg
     2641 ttgaacggag cggtgacgtg cgtgaaagat tgcaggtctt cgaaccgccg cacattcgca
     2701 ttggcggtat cgacagcggc gagtttgagt tttaagtcgg cctgcttctc ggcattttct
     2761 tgttcgctga cgctcgcggt cttgagcaag ccagcccacc ggtccgcggt gattttggca
     2821 agggacgagg cggcttcggc ctgggccaat tccgctttcg cgccgctcaa ttgctgttcc
     2881 aattcgggtg tgtcaatgtc cgcaagcgat tggccggctt caacgcgtcc gccgatgtcc
     2941 acgtaccaac gcttcaaaaa tccgctggcg cgcgcataaa tgggcgcatc gacaaacggc
     3001 ctcacttcag cgggcaaaat caggccgggc gtcgccttgc cgggcgcggc ggagatgacc
     3061 tgcactgttg gcgtggacaa ttcgacggtc tctttcgcaa gttcgctgcg gtgcatcaaa
     3121 cgcggcacca aacccgcgat cagcgcggcc aacaaaacca ggacgaccag caacatcaaa
     3181 cggccaagct tgggtggcga tgtatttgcg ccgttgccgg ccagtccttc ggaccgggcc
     3241 ggaagcggcg cttcggcgag cgatgatgtt ttgttgttct taagcgtgtt catgggtgga
     3301 atgtaaaata tttgtttcca aatcagcggc gggagccatg ccaggcgcgg tgttgcgatg
     3361 catgatggtg aaaacgaccg gcacgaaaaa taacgtggcg atggtcgcca caaaaagtcc
     3421 gccgatgacc gcgcgtccca aaggcgcatt ttgttcgccg ccttcgccca atccaaggct
     3481 caacggcaac atgccgatca ccatcgcgag ggccgtcatc aaaaccggcc gcaaccgtcc
     3541 cgcgcccgcc gtcaacgcgg ctttcagcgg cggcattcct tgttgcatgt tgagccgcgc
     3601 gaatgtgacc accagcacgg cgttggacgt cgccacgccg aggctcatga tggcccccat
     3661 caacgccggc acgctcaatg tggtgaacgt gagatacaac gcccaaatca ctccggccag
     3721 cgcgccgggc aaggccatga tgatgatgaa cgcgtcaagc cagctttgga aattcaccac
     3781 gagcaggaaa taaaccagca gtacggcccc gaccatgccg acacccagcc cgacgaaact
     3841 ggagtgcatc gtttcagccg cgccgcgcaa ttttatttcc gtgccgcgcg gcagattttt
     3901 ttgcgtatct ttgagaatcc gttgcaaatc gcccagcaca ccgcccaagt cccgtccgct
     3961 cacgttgccg aaaatgtcaa tcaccggcac ggcgttgtaa tgggaaaaca ccggcgaccc
     4021 ggcgatacgg gtcatggtcg agacattcgc cagcacctgg ccgttcccct gccccgcgcc
     4081 gccgccgctg acgggcaatg atttcagcgc cgcgacggaa tccagcacgc gttccggcgc
     4141 gcgcgcgttg atcaagtatt ggatgccgta ttgagtgttc agccagtacg caggcgtcac
     4201 ctgtccgctg ccgctgacgc tcaacaaaac ctggttcgca acgtcgcgct cggaaaggcc
     4261 gatggccgct gatttcgtcc ggtcaatgtt gaagcgcaac ttgggcaggt cgccgggctg
     4321 ttggattcgg atgtccaccg cgccggggat gttgcgcatt tggtcggcca attttgcggc
     4381 gatctcgcgg ttcttcgtgc ggtcccagcc ggtgagttga acattgagcg gcgcgggaat
     4441 gccgaagctc aacgtctggc tcacgatgtc cgcaggcaga aaataaaaca tcgtgctcgg
     4501 aaaatcgcgg ttcaaatccc gccgcaattt ccgggtgtag ccttcggtcg gatgatgatg
     4561 gccttccttc aacgaaatca ggatgtccga atcactcgcg ccgatgagcc cgttattgct
     4621 gtaactgagc gcgatgctgg agttgggaat gccgatgttg tcgagtatgc cttcaagttc
     4681 gtcggcggga atttcgcggc ggatgaaatc ttcgacgtcc gcgacgagtt tcgcggtttc
     4741 ctcgacgcgc attcc
//
LOCUS       DASZMM010000261         5511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3660675, whole genome shotgun sequence.
ACCESSION   DASZMM010000261 DASZMM010000000
VERSION     DASZMM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3660675"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1462)
                     /locus_tag="VG754_06305"
     CDS             complement(74..1462)
                     /locus_tag="VG754_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWF18860.1"
                     /translation="MVIFCVLAGCAVGPNYKRPKVNAPSNFRGEEQIGTNSFGDLPWW
                     EIFHDENLQGLIRTALSNNYDLRVAVTRVIQAQAVTAETRAQFFPQVNYTGVAGSGKN
                     TIPGSTSAFPTGGSNTTLFQGDLNASWEIDLWGRIRRMTESARAQYFATQEARRDVTT
                     SLIAQVAQDYFQMLALENELAIERDATNAYAGSLKIFSQRLHEGAASKLETSSAEALL
                     DSAAATIPDLERQIAQLENQISVLIGENPRAIPHDSSKLENQFPPDVPAGLPAALLER
                     RPDVREAEQQLHSANAQVGVAKSDFFPQLSLTGLFGRVSPEVATLTSGTALAWGAAGT
                     LTGPLFQGGRLRAQYKQALAARDQYALQYQSTVLNAFQEVSSALIARQKLAESRFLHA
                     RAVESYQEAVKIVNQRYQLGQSSYYEVLQEEQLLYPEENSLVLTELNQLSSIVQLYRA
                     LGGGWRTEKQTE"
     gene            complement(1551..4712)
                     /locus_tag="VG754_06310"
     CDS             complement(1551..4712)
                     /locus_tag="VG754_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF18861.1"
                     /translation="MYRFFINRPIVAMVIAILMVIIGSVTMFTLPIAQFPNIVPPEIR
                     IQTTYVGADAQTLAESVATPIEEQMSGVDNMNYMYSISANNGVMRMGVNFDVKTDPNI
                     DQVLSQLRENQSEPQLPADVRNFGITLLKSPTTPLMLVAIVSPEGTHDATFLANYAYI
                     NLVDRITQLKGISNVQVFGAGQYAMRFWVKPDQLAKLNVTITDIVNAINAQNSVNPAG
                     QVGGPPTPKGQEFTYTITAQGRLVTEQEFGNIVIRENPDGSVVRLKDVARIELGAQTY
                     SLVGKLNGRPSAVLAIYQLPGSNAIDAAKGVRKLMDEMKAAFPGDLDYVVSLDTTLAV
                     SEGIREIMHTLIIALILVIIVVFIFLQGWRASLIPLIAVPVSLIGTFAFFPLFGFSIN
                     TLSMLGLVLAIGLVVDDAIVVVEATERHIEEGENPKDAAIKAMDEVAGPVVALALIMA
                     AVFVPTVFIPGITGRLYQQFAMTIVISVALSAFNALSLSPALAAKLLRPRKHNEYRGP
                     LAKFYCWFNNKFDKVREEYLHISRALVNHSVLALVSLVIIAVVAVLLAKRLPTAFVPE
                     EDQGYAFAMAQLPSAASLERTDAVCKQIEKILANTPGVRYYTTVEGFSLLSQVQATYS
                     AFFFVTLKPWSERTKAAEQSIPIRKRMNDDMSKLPQASAFAFPPPSIPGIGTSGGATF
                     MLEDRSGAQDLSYLKQNLDKFMSAASKRKELAGLTTTFLPSVPQMFAKVDRDKVLKQG
                     VAVTDVYRTLQTFMGGNFVNYFNRFGRQWQVYIEAEAQYRASADDLRLFYVRNSAGQS
                     VPLSAFTEIQRRSGPEFILHYNEYPTAQINVPPSTAYSSSQVNQALEEVFAQTMPKQM
                     GFDYFGMSFQEKKAAEGVSPSLIFTLSIICVFLILAAQYESWSLPFSVLLGTPTAILG
                     AFVALNVRSFQNNVYAQIGLIMLIGLAAKNAILIVAFAQKEYEEGGKSIIDAAEAGAS
                     IRLRPILMTSFAFVLGCLPLWMASGSGAISRRTLGTVVIGGMLGATFVDTLIVPVTFY
                     VVEKLAAGCSRKPHVPKPPDPTG"
     gene            complement(4717..>5511)
                     /locus_tag="VG754_06315"
     CDS             complement(4717..>5511)
                     /locus_tag="VG754_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF18862.1"
                     /translation="RYTPLAKQQAISQQTLDDALQSNLSAEAQVKADEATVESAKLNL
                     SFTRITSPVDGLAGIAIAQVGDLVGPSGNALTTVSTIDPIKDYFQATEQSYLELWRYL
                     AAPSGSNMDVPLELILADGTAYPEKGKFFSADRQVNPTTGTLQVVGTFPNTNFLLRPG
                     QYGRVRAQTTVKTNVFFVPARAVNQLQGTYQVTLVVTEGTTNKAHIQNVKIGPQMGAN
                     WVIEAGLKPGDRVVVEGTQKAKEGAAITPKPLEAAATNSPPAQASN"
BASE COUNT         1182 a         1551 c         1565 g         1213 t
ORIGIN      
        1 aattgggatg agatgctctc gccttgaacc catcactcca atactccact actccagttc
       61 caaaatctct ccattactcc gtttgttttt cggtccgcca tccaccaccg agggcgcgat
      121 agagctggac gattgaggag agctggttga gttcggtcag caccaatgaa ttttcctccg
      181 ggtaaagcag ttgttcctcc tgtaaaactt cataataact cgattggccg agttggtaac
      241 gttggttcac gattttcacc gcttcctgat acgactcaac cgcgcgtgca tgcaagaagc
      301 gcgactcggc gagtttttga cgggcaatca acgcgctgga gacttcctga aaggcgttca
      361 acaccgtgga ctgatattgc agcgcgtatt ggtcccgcgc ggcgagggcc tgcttatatt
      421 gcgcgcgcaa ccgtccgcct tgaaaaagcg gccctgtaag ggtccccgcc gcgccccatg
      481 ccagggcagt tccgctggtg agagtggcaa cctccgggct gacgcgcccg aaaagcccgg
      541 tcagcgacag ttgcggaaag aaatcggact ttgccacgcc gacttgcgca ttggccgaat
      601 gcaactgttg ctccgcctcc cgcacgtccg ggcgtcgttc cagcagtgct gctggcaatc
      661 cagcgggcac gtccggcgga aattgatttt cgagcttgga actatcatgc ggaatagcgc
      721 gtggattttc cccgatcaag acgctgattt ggttttccaa ttgagcgatc tgacgttcga
      781 gatcgggaat cgttgcggcg gcggaatcga gcaatgcttc agccgaggaa gtttcgagtt
      841 tcgaggccgc gccttcatgc agccgctggc tgaaaatttt aaggctgccg gcgtaagcgt
      901 tggtggcgtc ccgctcgatg gcgagttcgt tctccaatgc gagcatttgg aagtagtcct
      961 gcgcgacctg cgcgatgagc gatgtggtca cgtcgcgccg ggcctcctga gtggcgaaat
     1021 attgcgcgcg cgcggattcg gtcatgcggc gaatgcgacc ccacaaatca atttcccagg
     1081 aggcgttgag gtctccttga aaaagcgtcg tgttcgaccc gcccgtcggg aatgcgctgg
     1141 tgctgccggg gatggtattt tttcctgagc ccgccacccc ggtgtagttc acctgcggaa
     1201 aaaactgcgc ccgcgtttcc gcagtcacgg cctgcgcctg gatgacgcgc gtcacggcca
     1261 cacgaagatc gtagttgttc gagagcgcgg tgcggatcaa accttgcagg ttctcgtcat
     1321 gaaaaatttc ccaccacgga agatcgccga aagaattggt gccaatttgt tcttcaccgc
     1381 gaaaatttga cggcgcgttg actttcggac gtttataatt cggacccacg gcgcaaccag
     1441 ccaaaacgca aaaaataacc atcaagacac caagacacca ggacgggaaa cggttttctc
     1501 ctttgtgcct tggtgtcctg gtggttgatg tttcaaacct gaattccata tcagccggtc
     1561 gggtcaggtg gtttcggcac gtgtggtttt ctgctgcacc ccgccgcgag tttttcgacg
     1621 acgtaaaagg tcacaggcac aatcaacgtg tccacaaacg tcgcgcccag cattccgccg
     1681 atgaccaccg tgccaagcgt gcgccgtgaa attgcgcccg aaccggaggc catccagagc
     1741 ggaagacagc cgagcacaaa cgcgaatgag gtcatcaaaa tcgggcgcaa acggatgctc
     1801 gcgcccgctt ccgcggcgtc aatgatggat ttgccgcctt cctcatattc tttttgcgca
     1861 aacgcgacga taaggatggc attctttgcg gcgaggccga tgagcatgat gagcccgatt
     1921 tgcgcataaa cattgttttg aaaactacgc acattcaacg ccacaaatgc gccgagaatg
     1981 gcggtgggcg ttcccaaaag cacgctgaac gggagcgacc agctttcata ttgcgcggca
     2041 aggatcagga agacgcaaat gatcgaaagg gtaaaaatca agctcggcga aacgccctcg
     2101 gcggccttct tttcctggaa ggacatgccg aaataatcaa agcccatctg cttgggcatg
     2161 gtttgcgcaa aaacttcttc aagcgcctgg ttgacctggc tggagctgta ggcggttgag
     2221 gggggcacat tgatttgggc tgtaggatat tcattgtaat gcaagataaa ttccgggccg
     2281 ctgcggcgtt ggatttcggt gaacgcggat agcggcacgc tttggcccgc gctattgcgc
     2341 acataaaaca accggagatc gtcggcgctg gcgcggtatt gggcctcggc ttcgatatag
     2401 acctgccact ggcggccaaa gcggttgaaa taattcacga agttgccgcc catgaaggtt
     2461 tgaagcgtcc ggtaaacatc agtgacggca acgccctgtt tcaacacctt gtcgcggtcc
     2521 accttcgcga acatctgcgg cacgctgggc agaaaagtcg ttgtcaagcc ggcaagttcc
     2581 ttgcgcttcg aagccgccga catgaatttg tcgagatttt gtttgagata ggaaagatct
     2641 tgcgcaccgg aacggtcttc cagcatgaaa gtcgctccgc cggaagtgcc gatgccgggg
     2701 atggaaggcg gcggaaaagc gaaggcagaa gcttgcggca gcttcgacat gtcatcattc
     2761 atgcgcttgc gaatggggat ggattgttcg gcggcttttg tccgttcact ccacggcttg
     2821 agcgttacga aaaagaacgc gctgtatgtc gcctgcacct ggctcaacaa gctgaaaccc
     2881 tccaccgtcg tgtaataccg gacgcccggc gtgttcgcca aaatcttttc gatctgcttg
     2941 caaacggcgt cggtgcgttc cagcgacgcc gcggacggga gttgcgccat tgcgaaagcg
     3001 tagccctggt cttcttccgg cacgaacgca gtcggcagac gttttgcaag cagcactgcc
     3061 accaccgcga tgatgaccag cgacaccagc gccaaaacgc tgtgatttac cagggcgcgg
     3121 ctgatgtgca ggtactcctc cctgaccttg tcgaacttgt tgttgaacca gcaataaaat
     3181 ttcgccaatg gcccccgata ttcgttgtgt tttcttggcc gcaatagttt cgcggcgagc
     3241 gcagggctga gcgaaagcgc gttgaatgcg gaaagcgcca cggaaatgac aatcgtcatc
     3301 gcaaattgct gatacaatcg tcccgtgatc ccggggatga acaccgtcgg cacaaacacc
     3361 gccgccatga tcaacgccag cgccaccacc gggccggcga cttcatccat cgccttgatt
     3421 gcggcgtcct tgggattttc gccttcttca atatggcgtt cagtggcttc gacaacgaca
     3481 atcgcgtcat ccaccaccaa cccgatcgcc agcaccaacc cgagcatcga aagcgtattg
     3541 atggaaaatc cgaacagcgg gaaaaatgcg aatgtgccga tgagcgaaac cgggaccgct
     3601 atcagcggga tcaacgatgc ccgccaacct tgaaggaaaa taaaaaccac gatgatgacg
     3661 agaatcagcg cgatgatcag cgtgtgcatg atttcacgaa tgccttccga aaccgcaagt
     3721 gtcgtgtcga gcgaaaccac gtaatccaga tctccgggaa aagcagcctt catttcgtcc
     3781 atcaatttcc tgacgccttt cgccgcatca atggcattgg aaccgggcaa ctggtagatc
     3841 gccaagacgg ccgaagggcg gccattcaat ttgccgacca aactgtatgt ttgcgctccc
     3901 agttcgatgc gcgcgacgtc tttcagccgg acaaccgacc cgtccggatt ttcgcgaatg
     3961 acaatgttcc cgaattcctg ttccgtgacg agccggcctt gcgcagtgat cgtgtaggtg
     4021 aattcctggc ccttgggcgt gggcggtccg ccgacctgtc cggcgggatt gaccgagttc
     4081 tgcgcgttga tggcgttgac gatgtcagtg atggtgacgt tgagcttggc gagttgatcg
     4141 ggcttgaccc agaaacgcat cgcatactgt cccgcgccga acacctgcac gttggaaatg
     4201 cccttcaact gcgtgatgcg gtccacgaga ttgatgtaag cgtaattagc gagaaacgtc
     4261 gcgtcgtgcg tgccctccgg cgaaacaatc gcaacgagca tcagcggcgt ggtcggcgat
     4321 ttaagcaatg tgatgccgaa attgcgcacg tccgcgggca attgcggttc cgactggttt
     4381 tcccgcaact gcgacaagac ctgatcgatg ttggggtcgg tcttgacatc gaaattcaca
     4441 cccatgcgca tcacgccatt gtttgcgctt atggaataca tgtaattcat gttgtccacg
     4501 ccgctcatct gctcctcgat gggcgtggcg accgattccg caagcgtctg cgcatccgcg
     4561 ccgacgtaag tcgtctggat gcggatttcc ggcggcacaa tgttcgggaa ttgcgcgatg
     4621 ggcagggtga acatcgtcac gctgccgatg atcaccatca aaatggcgat caccatggcg
     4681 acaatggggc ggttgatgaa aaagcggtac atgcgcttaa ttgctcgctt gcgccggtgg
     4741 cgaattggtc gccgcggctt ccaacggttt gggcgtgatc gcagcgcctt ctttagcctt
     4801 ttgcgttccc tcgaccacga cgcggtcgcc gggcttgaga ccggcttcaa tgacccagtt
     4861 agcgcccatt tgcgggccga tcttcacatt ttggatatgc gctttgtttg tggtgccttc
     4921 cgtcactacc agcgtcacct ggtaagttcc ctgtaactga ttcaccgcgc gcgccggcac
     4981 aaagaagaca ttcgttttca ccgttgtttg cgcgcgcacg cggccatatt gcccggggcg
     5041 aagcagaaaa ttcgtattcg gaaacgtccc caccacttgc aacgtcccgg tcgtcgggtt
     5101 cacctgccgg tccgcgctaa aaaattttcc cttctccgga taagccgtgc cgtcggccag
     5161 aatcaattcc agcggcacgt ccatgttact gccgcttggc gcggcgagat aacgccaaag
     5221 ttccagatac gattgttcgg tcgcctggaa ataatccttg atggggtcga tggtcgaaac
     5281 cgtcgtcaat gcgttgcctg aaggcccgac gagatcgccg acttgcgcga tcgcgattcc
     5341 cgccagtccg tccaccggcg aggtgatgcg cgtgaaactg agattcaact tcgcgctttc
     5401 aacggtggct tcgtccgcct tgacctgcgc ctccgcggag aggttcgatt gcagcgcatc
     5461 atcgagtgtt tgctggctga tggcctgttg cttggcaagc ggggtgtagc g
//
LOCUS       DASZMM010000262         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1487215, whole genome shotgun sequence.
ACCESSION   DASZMM010000262 DASZMM010000000
VERSION     DASZMM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487215"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="VG754_06320"
     CDS             <1..1920
                     /locus_tag="VG754_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWF18863.1"
                     /translation="DEGLINKNGQSIFVDSNQMVWAGFAASGGQWTPGLLQMQNDRFE
                     MANGYASTNNQEISAIFQDHAGKVWVGTQGGLACWNGQDWKIYTERNGLSSDAAQAIA
                     EDHNGDLWIGTGIGLNRLHDGHFTAFHKKDGLPDEDVSSLYMDGEGVLWIGTRGGGLG
                     RYFHGKWTRYTKKEGLLSDSIGYLLEDGQGNLWIGSLKGLMRVRKKELNDVAQGGANP
                     LACRAYGEADGMAASECTIGVQPGACRGHNGKLWFPTIKGIVSVDPAQIKINTNPPPV
                     VIESVSVSGESVVNSDEAEATNVLRSSVPSLITVPPGRERVEIQFTGLSFSSPKKARF
                     KYRMEGYEKGWTEVDGNVRTVIYRKLTPGNFTFHVTACNEDQIWNESGASLAFIVQPP
                     FWRTWWFLSLVTIIVIGWISGIVHYFSTQRLQRQLEGMRQQQALEKERARIARDIHDQ
                     LGASMTQVSLLGEMVESDKDDPQEVEAHARQISQTARDTSRVLDEIVWTVNPSNDTLE
                     GLINYICKHAQEYFAVAGLRYRLDVPSQLPVTDISPEVRHNVFLTAKEAITNVVRHAK
                     ATEVWLRLKLEPTRFILELQDNGRGPSGLEGKSTRNGLRNMHKRMEDIGGSFALTAAP
                     EGGALVRLTVPINNR"
     gene            1927..>2531
                     /locus_tag="VG754_06325"
     CDS             1927..>2531
                     /locus_tag="VG754_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF18864.1"
                     /translation="MATTVSIVEDNEQLRGTLAKFINREDGFSCVSQYPTAEAAIEGL
                     PRDKPQVVLMDINLPGMNGVECVRRLKQLVPDTQIVMLTAYEDTENIFNALAAGANGY
                     LLKRAPRAELLDALREVCRGGSPMTTHIARKVVQSFQKSAASAKPTENLSAREQEVLD
                     CLSQGFLYKEIAEKLGISYETVHTYIRRIYEKLQVRTRTEAV"
BASE COUNT          617 a          656 c          749 g          509 t
ORIGIN      
        1 gacgaagggt tgatcaacaa gaacggccaa tccatttttg tggacagtaa tcaaatggtt
       61 tgggcggggt ttgcggcgtc aggaggtcaa tggacacccg gattgttgca aatgcagaat
      121 gaccgcttcg aaatggccaa cggttacgcc agcacaaaca accaggaaat atctgccatc
      181 ttccaagacc atgctggcaa ggtttgggtc ggaactcaag gcggtttggc gtgttggaac
      241 ggtcaagact ggaaaattta tacggagcgc aatgggttgt catcggacgc cgcacaggcg
      301 attgccgaag accacaatgg cgatctttgg ataggaacgg gcatcggatt gaaccgcttg
      361 catgacggcc atttcacggc ttttcacaaa aaggatggat tgccggacga agatgtttcc
      421 tcgctctaca tggatggcga aggcgtgctt tggattggga cgcgcggtgg aggattgggc
      481 cgctattttc atggcaaatg gacgcgctat acaaaaaagg aaggattgct gagcgacagc
      541 atcggttatc tgctggaaga tgggcagggg aacttgtgga tcggttcgct caagggcctg
      601 atgcgcgtca ggaaaaagga attgaacgac gtagctcaag gaggggcgaa cccgcttgca
      661 tgtcgagctt acggggaagc cgacggcatg gccgcgagcg aatgtaccat cggtgttcaa
      721 ccgggtgcct gccgcggtca caatggcaaa ctttggtttc ccaccatcaa gggaattgtg
      781 tccgtggacc cggcccaaat caaaatcaat accaaccctc cgcccgtggt gattgaatca
      841 gtttcagtca gcggcgaatc cgtcgtgaat agcgacgaag cggaggccac caatgttttg
      901 cgctcatcgg ttccgagctt gatcacggtg ccacccggtc gggaacgcgt cgaaatccaa
      961 ttcaccggac tcagtttctc cagcccaaaa aaagcgcgct tcaaatatcg gatggaaggt
     1021 tacgagaaag gctggacgga ggtggacggc aacgtccgca cggtgatcta tcgcaagttg
     1081 actccgggca attttacgtt ccatgtcacc gcctgcaatg aagatcagat ctggaacgaa
     1141 tccggcgcat cgctggcatt tatcgtccag ccgccgtttt ggcggacgtg gtggttcttg
     1201 agtttggtga ccataatcgt gatcggatgg atcagtggca tcgtgcatta tttttcgacg
     1261 caacgattgc aacggcaatt ggaagggatg cggcagcagc aggcgttgga aaaggagcgc
     1321 gcgcgcatcg cccgcgacat ccacgaccaa ctcggcgcga gcatgacgca ggtttcgttg
     1381 ctgggtgaaa tggtcgaaag cgacaaggac gatccgcaag aagtggaagc ccatgcgcgg
     1441 cagatttccc aaaccgcgcg cgacacttcg cgagtgctcg acgagattgt ctggacggtc
     1501 aatccttcca acgacacgct cgaaggcctt atcaattaca tctgcaaaca cgcacaggaa
     1561 tatttcgccg tggcgggact gcgctaccgg ctggatgtcc cgtcacaatt gcccgtaact
     1621 gatatttcgc ctgaagtgcg ccacaacgtt tttctcaccg cgaaggaagc gatcaccaac
     1681 gtggtgcgtc acgcaaaagc aaccgaagtg tggttgcgtt tgaaattgga acctacccgg
     1741 ttcatccttg aacttcagga caacgggcgc gggccttcgg gactcgaagg caaatccacc
     1801 agaaatggat tgcgtaacat gcacaaaaga atggaagata tcggcggcag ctttgcgctc
     1861 accgccgcgc cggaaggcgg cgcgctggtg cggttgactg tcccgatcaa caaccgttga
     1921 acaatcatgg ccacaaccgt ttcaatcgtc gaagacaacg agcaattgcg gggcacactc
     1981 gccaaattta tcaatcgcga ggacggtttc agttgcgtca gccagtatcc caccgccgag
     2041 gccgccatcg aaggattgcc gagggacaag ccgcaggtgg tcttgatgga catcaatctt
     2101 cccggcatga acggcgtcga atgcgtgcgg cgtttgaagc agcttgtgcc cgacacgcag
     2161 atcgtgatgc tcacggctta cgaggacacg gaaaatattt tcaacgcgct cgccgccggt
     2221 gcgaatggct accttctcaa gcgcgcgccg cgcgccgagt tgctggatgc gttgcgcgag
     2281 gtttgccgcg gcggttcacc gatgacaacc cacatcgcgc gcaaggtggt gcaatcattt
     2341 cagaagagcg ctgcctccgc aaagccgact gagaatctgt ccgctcgcga acaggaagtg
     2401 ctggattgcc tgagccaggg ttttctctac aaggaaatcg cggaaaaact gggcatcagc
     2461 tatgaaaccg tgcacacgta catccgccgc atttacgaaa aattgcaggt gcgcacgagg
     2521 acggaagcgg t
//
LOCUS       DASZMM010000263         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_664713, whole genome shotgun sequence.
ACCESSION   DASZMM010000263 DASZMM010000000
VERSION     DASZMM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664713"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VG754_06330"
     CDS             complement(<1..194)
                     /locus_tag="VG754_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWF18865.1"
                     /translation="MNSTTNPRGIIFDCDGTLADTMPLHWKVWQEITRRHKLHFEEDR
                     FYALGGVPSRDILKMLGKEQG"
     gene            complement(211..1290)
                     /gene="prfA"
                     /locus_tag="VG754_06335"
     CDS             complement(211..1290)
                     /gene="prfA"
                     /locus_tag="VG754_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWF18866.1"
                     /translation="MDLRPHIERFARRLAEVEAALSDPKVFDNTLRAQELSKEYSQLK
                     ELVASGQGYLKALAELEENRTLLQTETADSELALMAKEDIGRLETEVKQLGHEVQKGI
                     LPPDPTDSRNTIFEIRAGAGGSESALFAADLYRMYTRYAEERGWKVHSLDASSSDLGG
                     FKEVIFGITGTDVFKRLKYESGVHRVQRVPATEAQGRVHTSTCTVAVLPEAQEVDIEI
                     RLEDLEINVCRASGKGGQGVNTTDSAVQIQHKPSGLIVRCADERSQQKNKASAMSVLR
                     SRLLERKVAEENAKYAAQRKEQVGTGERNERIRTYNFSQNRVTDHRIELTLYNLPAIL
                     EGDLDPIIDPLMAHDLDEKMAALQL"
     gene            complement(1460..1702)
                     /gene="rpmE"
                     /locus_tag="VG754_06340"
     CDS             complement(1460..1702)
                     /gene="rpmE"
                     /locus_tag="VG754_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWF18867.1"
                     /translation="MRVDIHPKYQESEIRCACGNVIKTRSTRPVILLGICNACHPFYT
                     GTQKFVDTAGRVDKFQQRMAKTQAAQAASAAKKKKK"
     gene            complement(1910..2551)
                     /gene="thiE"
                     /locus_tag="VG754_06345"
     CDS             complement(1910..2551)
                     /gene="thiE"
                     /locus_tag="VG754_06345"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWF18868.1"
                     /translation="MKPLSECRLYTFVDAAYLHGRAPEWIAQQLCDGGSDLIQLRAKN
                     SSPDEIRKMAEAILPVTRRAGVGLVINDHPDIAQELGAELCHLGQEDFFDSGRKHVEE
                     LKNKSSKLQIGLSTHTPEQARRALAAEPDYIAIGPVYATGTKPTAKPVTLDYVRWAAA
                     NVTIPWFAIGGINLKNLDEVIEAGARRICVVSAILNATDVAKACEEFKKKLCK"
     gene            complement(2570..>3538)
                     /locus_tag="VG754_06350"
     CDS             complement(2570..>3538)
                     /locus_tag="VG754_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18869.1"
                     /translation="RGIPVTIWEAGTYPRHRVCGEFISGRGHGTLARLGLREKLLQAG
                     AIPARTAIFFSESAASPVRELSEPALSISRFVLDDLLAKEFRQLGGELRESERWRGAE
                     FAEGMMRASGRRVQPAVNGWRWFGLKVHARRVALKADLEMHLSSSGYLGMSRLTGGEV
                     NICGLFRRPARARDGLANWQDILRGAEGSHRRERLREAEFDKSSFCSVAGLSLEPRRA
                     AAFEDCCIGDAITMIPPVTGNGMSMAFESAEMAIAPLAQYSEGKISWNEARQTIAQNC
                     DVTFARRLAWAAILQRLLFIAQLQTASVLALPRWQWLWRLLVEKTR"
BASE COUNT          757 a         1055 c          909 g          817 t
ORIGIN      
        1 ccctgttcct taccgagcat tttgaggatg tcgcgcgaag gcactccgcc aagcgcgtaa
       61 aatcggtcct cttcaaaatg cagtttgtga cgacgcgtga tttcctgcca gactttccaa
      121 tgcaacggca tggtgtctgc cagcgtgccg tcacaatcga agatgatgcc gcgaggattt
      181 gtggttgagt tcatggaaaa tgaaatttga ttatagttgc aatgccgcca ttttctcatc
      241 caagtcatgc gccatcaagg ggtcgatgat cggatccaaa tcgccttcca aaatggccgg
      301 cagattatag agcgtgagtt cgatgcgatg gtcggtcacg cggttctggg aaaaattgta
      361 ggtgcggatg cgttcgttgc gctcgccggt gccgacctgc tccttgcgtt gcgcggcgta
      421 tttggcattt tcctcggcga ctttgcgttc aagcagacgc gagcgcagca cgctcatggc
      481 gctggccttg ttcttctgtt gcgaacgttc atcggcgcaa cgcacgatca gaccgctggg
      541 cttgtgctgg atttgcacgg cggaatcagt ggtgttcacg ccctgcccgc ccttgccgga
      601 agcgcggcag acgttgatct ccaaatcttc cagccggatt tcaatgtcca cttcctgcgc
      661 ttcaggcagc acggcgactg tgcaagtgct ggtatgaaca cggccttgcg cctcagtcgc
      721 cggcacgcgc tggacgcgat gcacgccgct ttcgtatttc aagcgcttga aaacgtccgt
      781 tcccgtgatg ccaaaaatca cttccttgaa cccgcccaaa tcagacgaac tcgcgtccaa
      841 gctgtgcacc ttccagccgc gctcttccgc gtaacgcgtg tacatgcggt aaagatcggc
      901 ggcgaacaat gccgactccg aaccgcccgc gcccgcgcgg atttcgaaaa tggtgttgcg
      961 cgaatcggtg ggatcgggcg gtaaaattcc tttttgcacc tcatgaccaa gttgtttgac
     1021 ttccgtttcc aaacgtccga tgtcctcttt cgccatcaag gccagctcgg aatcagccgt
     1081 ttcggtttgc aatagagtgc ggttctcctc cagctccgca agtgccttca agtaaccctg
     1141 gccgctcgcg accagttcct tgagttgcga atattctttc gacaattcct gcgcgcgcag
     1201 tgtgttgtcg aaaactttcg gatcgctcaa cgcggcctcg acttccgcca aacgtcgtgc
     1261 gaatcgttcg atgtgtggac gcaaatccat ttctttaaag tcagccgaaa ccgaatgcag
     1321 tggtccggcg gttatttttt atcgcttgtt cccgtcgaag atggcgccgc tttcactcac
     1381 ctgatggctc cgcttccccg ccaaaaaaac aatccgcccg catggcgaaa aaccacgcgg
     1441 gcggacgttg aaaacgatgc tacttctttt tcttcttggc ggcgctggcg gcctgggcgg
     1501 cctgcgtctt ggccatgcgc tgctggaatt tatccacgcg gccggcggta tccacaaatt
     1561 tttgcgtgcc ggtgtagaag ggatggcagg cgttgcagat gcccagcaaa atcaccggtc
     1621 tggtcgaacg ggtcttgatc acgttgccgc aggcgcagcg gatttccgat tcctgatatt
     1681 taggatgtat gtcaactctc ataaaaggct ggataaccta ccaagggctt gggatttgac
     1741 aagagcgaaa gtgattaaac tatcaaatcc tcccacagcc tcgactaccc tctacacaat
     1801 cccaagcacg gaactcaaaa atgagcgtac acaaaacaat gtaagttgtt tagcataagc
     1861 aacttttgac gattttaaaa ttgatcgttc tttggttttt tcgtcgtttc tatttgcaca
     1921 gcttcttctt gaactcttcg caggctttcg caacatccgt cgcgtttaaa atcgccgaca
     1981 ccacgcaaat gcgccgggcc ccggcttcga tcacttcatc caagtttttc aaattgatgc
     2041 cgccgatggc gaaccaggga atcgtcacgt tcgccgccgc ccaacgcaca taatccagcg
     2101 tcaccggctt ggcggtcggc ttcgtgcctg tcgcatacac tgggccgatt gcgatataat
     2161 ccggttctgc cgccaatgcg cgccgtgctt gttcaggtgt gtgcgtgctc aatccaattt
     2221 gtaactttga acttttattt tttaattcct caacatgctt gcggccgctg tcaaaaaaat
     2281 cctcctgccc aagatggcaa agttccgcgc ctaattcctg cgcgatgtca ggatgatcat
     2341 tgatgaccaa tccaacgccc gcgcgacgtg tcacgggcag gatggcttcg gccattttgc
     2401 ggatttcatc gggcgaagaa tttttcgcgc gcaattggat caggtccgag ccgccgtcac
     2461 aaagctgctg cgcaatccat tccggcgcgc gaccgtgcag atacgccgcg tcaacaaagg
     2521 tgtaaagccg gcattccgac aacggtttca tgaagcgaag actattcaat taacgtgtct
     2581 tttccaccag caatctccac aaccactgcc agcgcggcag cgcgagaacg gacgcggtct
     2641 gcaattgcgc aatgaacaac agtcgttgaa gaatggccgc ccacgcgagc cggcgcgcga
     2701 acgtcacatc acaattttgt gcaatggtct gccgcgcttc attccatgaa atttttcctt
     2761 cgctgtattg tgcaagcggc gcgatggcca tttccgccga ttcgaatgcc atggacatcc
     2821 cgttgcccgt gacgggcgga atcatcgtga tcgcgtcgcc gatgcagcag tcctcaaacg
     2881 ccgccgcgcg ccgcggttcc agcgacaatc cggcgaccga acagaatgag cttttgtcaa
     2941 attccgcttc gcgcaatcgt tcacgccgat gcgaaccttc tgcgccgcgc aaaatgtcct
     3001 gccagttcgc aagcccatcg cgagccctcg ccggacgccg aaaaagcccg caaatattga
     3061 cttccccgcc ggtcaaccgg ctcatgccaa gatagcccga agaggaaaga tgcatctcca
     3121 aatcagcctt caacgcgacg cgacgggcat ggaccttgag tccgaaccat cgccagccat
     3181 tgaccgccgg ttgaacgcgc cgtccgctcg cgcgcatcat gccctcggca aattccgcgc
     3241 cgcgccagcg ttcgctttcg cgcaattcgc cgccaagctg ccggaattct tttgcaagca
     3301 aatcgtccaa cacaaaacgc gagatggaaa gcgccggttc cgaaagctct cgaaccggtg
     3361 atgccgcgct ttcggaaaaa aaaatcgcgg tccgcgcagg aatcgctcca gcttgcaaaa
     3421 gtttttcgcg caatccaagc cgcgcaagtg ttccatggcc gcgtccgctg atgaattcgc
     3481 cgcagacccg atgccgcgga taagtgcccg cctcccaaat cgtcaccggt atgccgcg
//
LOCUS       DASZMM010000264         3980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_665012, whole genome shotgun sequence.
ACCESSION   DASZMM010000264 DASZMM010000000
VERSION     DASZMM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665012"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VG754_06355"
     CDS             <1..215
                     /locus_tag="VG754_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18870.1"
                     /translation="VLVAQIFNLLYRRLAVGKAVKNTGSFEISEIVIPSRGLIVSVDG
                     RNKTFLKKAPPRTGVVGRLAQRCRAA"
     gene            572..2026
                     /locus_tag="VG754_06360"
     CDS             572..2026
                     /locus_tag="VG754_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF18871.1"
                     /translation="MATLLLIDDEADVQYSFRRIFDSPEMELHTAASGEEGLKLIPRL
                     KPDLVIMDVRMGGISGLETLRRIREIDSKLLVILMTAYGTTQTAIEAMKLGAYDYLLK
                     PFDVPKLKAIIAGALKTALDMKQVVSYEPLLESEDYEVGIVGRSEPMQKVFKLIGQLA
                     GSDATALITGESGTGKELVARAIYHHSERSGKPFLAINCAAIPEQLLESELFGHERGA
                     FTGAATQRIGRFEQCHQGTIFLDEIGDMTPATQTKILRVLQSGTFERVGGNQAIQVDV
                     RIIAATNKPLEQAVAARQFREDLFYRLNVVRVQLPPLRERPEDIRLLVNYFLKKLATH
                     PEHKPKSISPGVIKALEKYHWPGNVRELENVIQRAVVVAKGDVILQGDLPPEITDPTG
                     HASSTAQLTAGPLAAGESGVTEIASVAKLLFRWARQDPKLKVFPAVERELIIQALQES
                     KGNQVHAAKLLGITRATLRKRVEKFNIKRELNIQ"
     gene            2032..2868
                     /locus_tag="VG754_06365"
     CDS             2032..2868
                     /locus_tag="VG754_06365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF18872.1"
                     /translation="MTQPEPRLLLLIPAYNEEDRIEPVLIDFAEYFQKIYTGTFQLVV
                     VLNGCKDNTCGVVQRVAAKYPSVSAMEFPEPIGKGGALIEGLKLAPLADLIGYVDADG
                     ATGPKAFHDLVRLTDKADCVIGSRWLPGAIIHQSQTSRRQFASRGFHYIVEMFFQMKI
                     RDTQCGAKVMHRRAVEKVHSSLRIADMAFDINLLYVLKRAGYKVLEVPTEWTDKIGSK
                     VVLGKTSLTMLLSVIRLRLFYSPFYNMLRPFKPLEAWIYKKLSQPPPRSGPGPDDDGG
                     KS"
     gene            complement(2963..>3980)
                     /locus_tag="VG754_06370"
     CDS             complement(2963..>3980)
                     /locus_tag="VG754_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18873.1"
                     /translation="VMLWTFCVWLATVIAAIIYRHEWVAALKLSNPMALWLTIAVGVV
                     MIWMQIYQGLLQGRQNILWFGWANISNGVGRVVIGGIIVVLLGGQAAGLMLGVFIGMV
                     AAFLACVSQNFDLLKSPSAKFDATGWLKRVVPLTLGFGVSTFLFSADAIVVQNYLGAN
                     GKAADYIFGATLCRAIVLFTVPLAAVMFPKLVHSAARSQKSNVMGMTMIGTVGLSLLA
                     VLGLALVSPTIMRIFSKGSHEDMMTMTRLIPIFAAGMVPLGSGNVLLYNLMAHSRFKI
                     VPALVVLAIGYWIALQHFHDSFKTVIQTFCVFTLIYFGLCALFTWVLDRGHGVVKEPV
                     VEGV"
BASE COUNT          916 a         1136 c         1117 g          811 t
ORIGIN      
        1 cggtccttgt agcgcagatt tttaatctgc tgtatcgccg acttgcagtc ggcaaggctg
       61 tcaaaaacac ggggagtttt gaaatctctg aaatcgtgat cccatcgcgc gggctcatcg
      121 tgagcgtgga tggccgcaac aaaacctttt taaaaaaagc gcccccgcgc acgggggtgg
      181 tcgggcgtct ggcgcaaaga tgccgtgctg catgaactgc cccaagtgca gccagtccgt
      241 ggacgcaaac gcaaatatgg caagctgctg gccactcccg aacaactgct caaagacgac
      301 cagacgcctt ttcaaagcgt gcgggccttt gccgccggcc gggcgcatga ctttcacccc
      361 ttcagggcgg agtcgaattt ctgcatcccg tccccggggc ttgcgttgcg cgctgcaccc
      421 cgggctgtca cattgcgggc cttcagcccg ccgcaacgcc cttttccgtt tcatcctaca
      481 atcttccgct tcgtaaccca ttcacatcct ctgcggataa cgattaaatt cattcgactt
      541 tcttcgcgag tcattcaaaa ttgagccaaa catggctacg ttgttgttaa tagatgatga
      601 agcggatgtg caatattcgt tccgccgaat ttttgattct ccggaaatgg aattgcacac
      661 cgccgcgagc ggcgaggagg ggctgaagct cattccgcgc ctgaaacctg acctcgtcat
      721 catggacgtg cgcatgggcg gcatcagtgg gctcgaaacg ttgcgccgca tccgcgagat
      781 tgattccaag ctgctggtca tcctcatgac ggcctacggc acgacgcaga cggccatcga
      841 ggcgatgaaa ttgggcgctt acgattatct gctcaagccg tttgacgttc ccaagctcaa
      901 ggccatcatc gccggcgcgc tcaagaccgc gctcgacatg aagcaggtcg tttcctacga
      961 gccgttgctc gaatcggaag attacgaggt gggcatcgtg ggccgcagcg agccgatgca
     1021 aaaagttttc aagcttatcg gccagttggc cggttcggat gcgacggcgc tcatcaccgg
     1081 cgaaagcggc accggcaagg aactggtcgc ccgcgcgatt tatcatcata gcgaacgcag
     1141 cgggaaaccg tttctcgcca tcaattgcgc cgcgattccc gagcagttgc tggagagcga
     1201 attgttcggg cacgaacgcg gcgcgttcac cggcgcggcc acgcagcgca tcgggcgctt
     1261 tgagcaatgt catcaaggca cgatttttct ggatgaaatc ggcgacatga cgccggcgac
     1321 ccagacgaaa atcttgcgcg tgttgcaatc cggcacgttc gagcgcgtgg gtggcaatca
     1381 agcaattcaa gtcgatgtgc ggatcatcgc ggcgacgaac aagccgctgg aacaggcggt
     1441 cgcggcgcgg caatttcgcg aggatttgtt ttaccggctg aatgtcgtgc gcgtgcaatt
     1501 gccgccgttg cgcgagcggc ccgaagacat ccgtttgctg gtgaattatt ttttgaaaaa
     1561 gctggcgacc catccggagc acaagcccaa gtccatttcg ccgggcgtca tcaaggcgct
     1621 ggaaaaatat cattggccgg gcaacgtgcg cgagttggag aacgtcatac agcgcgcggt
     1681 ggtggtggca aaaggcgacg tgattttgca gggcgatttg ccgccggaaa tcactgatcc
     1741 aactggtcac gcgagttcga ccgcgcaatt gaccgccggc ccgcttgccg cgggggaaag
     1801 tggcgtgacg gagatcgcgt cggtcgcgaa gttgttgttc cggtgggcgc ggcaagaccc
     1861 gaagttgaaa gtttttcccg ccgttgaacg cgagttgatc atccaggcgt tgcaggaaag
     1921 caagggcaac caggtgcatg cggcgaagct gctcggcatc acgcgcgcga cgttgcgcaa
     1981 acgcgtcgag aagttcaaca tcaagcgcga actgaacatc cagtaacacg cgtgactcag
     2041 cccgaaccca ggctattgct tttgattccg gcctacaacg aggaggaccg gatcgagccg
     2101 gtgttgattg atttcgcgga atattttcaa aaaatttaca ccggcacttt ccaattggtg
     2161 gtcgtcctca acggttgcaa ggacaacacc tgcggcgtgg tgcaacgcgt cgcggcgaaa
     2221 tatccctcgg tgagcgcgat ggaatttccc gagcccatcg gcaagggcgg ggcgttgatt
     2281 gaaggattga agctcgcgcc gctggcggat ttgatcggtt acgtggacgc agacggcgcg
     2341 accggcccga aggcatttca tgatttggtg cgattgactg acaaggccga ttgcgtcatc
     2401 ggttcgcgtt ggttgccggg cgcgatcatc catcagtcgc aaacgagccg tcggcagttt
     2461 gcgagccgtg ggtttcacta catcgtggaa atgttctttc agatgaaaat tcgcgacacg
     2521 caatgcggcg cgaaagtgat gcatcgccgg gcagtggaaa aagtccattc atcgcttcgc
     2581 atcgccgaca tggcgtttga catcaacctg ctttacgtgc tgaaacgtgc cggttacaaa
     2641 gtgctcgagg tgccgaccga atggaccgac aaaattggtt cgaaagtcgt gctggggaaa
     2701 acgtcgctga cgatgctgct ttccgtcatc cggctgcggc tgttttactc gcccttttac
     2761 aacatgttgc ggccgttcaa gccattggaa gcttggattt acaagaagtt gagccagcct
     2821 ccaccgcgtt caggaccggg gccggatgat gatggtggga aaagttaaat ggttaaatcg
     2881 ttacatggtt acaaggccgc gaacataccg acgctcatcc tttgtaacga tgtaaccatg
     2941 taacgattta acttcgcagc catcacactc cttccacaac cggctccttc accacgccat
     3001 gcccgcgatc caaaacccac gtgaacagcg cgcacagtcc gaagtaaatc agcgtgaaaa
     3061 cgcagaaggt ttggatgacc gtcttgaacg aatcatgaaa atgttgcaac gcaatccaat
     3121 aaccgattgc cagcacaacc agcgctggca cgattttgaa tcgcgaatgc gccatcagat
     3181 tgtagagcag cacatttccc gaaccgagcg gcaccatgcc cgccgcgaag atcggaatca
     3241 acctggtcat ggtcatcatg tcttcgtgac tgccctttga aaaaatcctc atgatggtcg
     3301 gcgaaaccag cgccaatccc agcaccgcga gcagacttaa accgaccgtc ccaatcatcg
     3361 tcatgcccat gacattgctt ttttgcgagc gcgccgcgct gtgaacgagc ttgggaaaca
     3421 tcaccgcggc gagcggcaca gtgaatagca caattgcgcg gcatagggtc gcgccgaaaa
     3481 tgtaatccgc cgccttgccg ttcgcgccga gataattctg caccacaatc gcgtccgcgc
     3541 tgaaaaggaa cgtgctgacg ccaaagccga gcgtcaaggg caccacgcgt ttcaaccaac
     3601 ccgtcgcatc aaattttgcg ctgggacttt tcagcagatc gaaattctgg gacacacaag
     3661 cgagaaatgc ggcgaccatc ccgatgaaca cgcccaacat cagcccagcg gcctgcccgc
     3721 ccagcaatac gacaatgatt ccgccgatga ccacccgccc gacgccgttg gagatgttgg
     3781 cccagccgaa ccacaaaata ttctgccggc cttgcagcaa tccctgataa atttgcatcc
     3841 aaatcatcac cacgcccacc gcgatggtca gccaaagggc catcggattg ctgagcttga
     3901 gagcggcgac ccattcgtgc cggtaaatga ttgcggcgat caccgttgcg agccagacgc
     3961 aaaacgtcca aagcatcacc
//
LOCUS       DASZMM010000265        11596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2021633, whole genome shotgun sequence.
ACCESSION   DASZMM010000265 DASZMM010000000
VERSION     DASZMM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021633"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VG754_06375"
     CDS             complement(<1..806)
                     /locus_tag="VG754_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HWF18874.1"
                     /translation="MDSKPNVIPHRGASENLPNRFEKIHLERDEDWNPDEDVLPQTQF
                     LRDHSSTIIATNDSPDVGFEASVNPYRGCEHGCIYCYARPTHEYLGFSAGLDFETRIM
                     VKDRAPELLRAELSSPKWKPKTISISGVTDCYQPVERKLKLTRGCLQVLAEFRNPVGI
                     VTKNHLVTRDIDVLSELARYNCSVVFVSVTTLDTDLRKVMEPRTSPPASRLAAIEALA
                     NAGIPVGVMVAPVVPGLTDHEMPNILAAAVKAGAKFAGYVTLRLPYAVAPL"
     gene            complement(1185..2462)
                     /locus_tag="VG754_06380"
     CDS             complement(1185..2462)
                     /locus_tag="VG754_06380"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HWF18875.1"
                     /translation="MADENPIASNAADDSKVQTWNWHAQNTDILDGHPGFSTKYNGPN
                     SLSPVGEVQDTISLDLMTGVRLWRGAEFHLDGLMWQGFGFSHTVGIEAFPNAEAYRVG
                     TQAPNVVFARVFLRQTIGLGGEQEKIEDDSLHLAGKEDISRITFTLGKFSAKDIFDNN
                     TYANDPRTQFMSWGLVANEAWDYPADSLGYDTGIAIEWNEPKTTLRYGFFQVPRVSNG
                     LAQDKNYTAAWAMVTEFERRFAFNDHPGAVRLLAYLNRAHMGSYEEVVDNPALNNDIR
                     LTREYRYKFGFGLNAEQELTKGIGAFMRLGWSDGRNEAWMYSDVDKAASLGLSVNGAF
                     WHRTNDTFAIAGVFSGASHIHQQFLTIGGTGILAGDGALNYGVEKELETYYDFGIWKT
                     IHGTLDYQFVSDPAFNRDRGPVHVLSARLHWEF"
     gene            complement(2655..4049)
                     /locus_tag="VG754_06385"
     CDS             complement(2655..4049)
                     /locus_tag="VG754_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HWF18876.1"
                     /translation="MKRNRKASLHYWAAILASTSAAASPAIADENPIASTNETQAQNW
                     NIHAQNTDIVQGDPGFPAKYSGPNSLHDGGEVRETVTLDLFAGARLWRGAEAHADFLI
                     WQGFGLSKTFGIEDFPNGDAYKAGTESPNYTFARLFLRQTIGFGGEQEDTPDGPLTLA
                     GKRDISRLIFTIGRFTPLDVIDNNAYAGDPHTQFMNWAFMANNTFDYGQDTVGYTTGM
                     AVELNQPKWAVRYTFFQMPREKNGFTGDDQYLMWPNRGAYGPFWREWAMAGEFERRYS
                     INAHPGAVRFLAWLDQANFASYAGATSILLANGPGADISPARAFRYKYGFGLNWEQEI
                     SKNIGLFSRLGWTDGHNETWTFTDVDYSASFGASIKGGAWDRPDDTWGLAFVASGASR
                     DNQKFLQAGGTDMLDGDGALTYGWEKALETYYDLKIWKTIHCSLDYQFVSNPAFNRDR
                     GPVHVFGARLHWTF"
     gene            4438..5511
                     /locus_tag="VG754_06390"
     CDS             4438..5511
                     /locus_tag="VG754_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HWF18877.1"
                     /translation="MRDESLDFLKTLVNTPSPVGHEARGQRVWLDYASKFADTTYSDA
                     YGNCVAVLNKGGSPRVMLAGHADEIAMSVNYIDPEGFIYVRKMGGVDAAITKAQRVII
                     HTRNGAVNGVVGNVAPHLSKHDDGDKKTPKIHDLFIDIGVSSTKDAEKLVQIGDPITL
                     NDEFQLLRNDLAVARAFDNRIGTFAVAEALRLLKESKGKLEAEVCAVSNIQEEVGLLG
                     ARQIAYSLKPDVAIVVDVTHATDYPTVNKAQHGNIKVGNGPTVTHGGCNHPEVVARIE
                     QLAKTKKIPLQHEAMSSTSGTDTDVIFWTRGGIPSALVSLPNRYMHSPVEVVSLSDLE
                     KIPELLAAFARSLKTGEEFKVKI"
     gene            complement(5515..6207)
                     /locus_tag="VG754_06395"
     CDS             complement(5515..6207)
                     /locus_tag="VG754_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18878.1"
                     /translation="MIKWIFRWAFRLLILLVILVVAGILLMDTFAREYVEYQIHRQTG
                     MEVKIEKVRIGLLNPQVTIENMVLYNTAAFGGSPFIEMPELHVEYDRDALVSRKLHCK
                     LMRFNLSRINLVEDKSGRRNFDMSQAQVVPPAAKSPNKNSASKTSGMQFAGIDTLNLT
                     LGKVTYLRMKQPDKVDEFKMNVDHQIFTGIKSEQDFSAVLMLALLKSGANVFQTGSNG
                     QNLLQLFAPPKK"
     gene            6459..7175
                     /locus_tag="VG754_06400"
     CDS             6459..7175
                     /locus_tag="VG754_06400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF18879.1"
                     /translation="MSNSSPQIPEFDRFATDYDRALAEGISVSGEDKDYFANGRVEWL
                     AGCLHKLGCHPKTVLDYGCGTGSATPFLARSLSFDSLVGVDVSVQSLVVARQQHGSPQ
                     VKFTSVNDYKPAGSVDLVFCNGVFHHIPPKDRHDAVKFIHASLKPGAFFSFWENNPWN
                     PGTRYVMSRIPFDRDAITLSPPEARRLLRAGGFEIVRTDFLFWFPRFLSWFRRLEKLL
                     SPLPMGAQYHVLCRKPHNRG"
     gene            7257..8960
                     /locus_tag="VG754_06405"
     CDS             7257..8960
                     /locus_tag="VG754_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18880.1"
                     /translation="MNSKSERVQSSVTASSKAIIIFLLILAAALRIWNLPALNEVRDW
                     DEIGYTTDGLMAWEGLLPGWESVPAGPQTWESWLFVGARSGWEFLHSQRKAATPGALK
                     PLMAIDQALFKTYEDLGPLRRLLLWISIVISLAGVYGAYRLGAKYGGVAGGLLMGGLA
                     AVLPLYLEYAGVAKSCSDAWMFSILAVSCAATMTGAKRCWWAGTMLGLAIGSRVDMAA
                     TVPLVFWGLWESHEKDSLWKSMAGTIALAMAIALLSAPFAMQGFVGILRQIVLSGSGT
                     YWGAEPQRLRTLKELAWDQGLGLIFIIAVVGLFFLPYGTRLKRGTLAAFAALVVVSIF
                     ARHHQPVRYYGAQIIVILVFIAVGAGALFRRWPKPALAFAMLLLIFPLVQSIRAAIAM
                     KAIHVPETSTEWVDAHVPAGTTVYVHPAFISRAVLPTEEAADAIWDIVTGNQAWRTKV
                     LDGFHRFAIPDRQLPRALSEDNLCKDRGVCRRWFILGGGQSNRPRYNLQLVPMSATFG
                     LQRETLGPEFQRTGGVVIWPTRASGLPGGLGEPFIKWVNYNGDGILVFVSPDVREKLT
                     H"
     gene            complement(8974..10182)
                     /locus_tag="VG754_06410"
     CDS             complement(8974..10182)
                     /locus_tag="VG754_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF18881.1"
                     /translation="MPLKPSERYAYQLIFMSDAYDITIVMPCLNEADTLATCIEKAHL
                     GLKRAGVRGEILIADNGSTDGSVQIAEKIGARVVHVKEKGYGNALKGGIEAAQARWII
                     MGDADDSYDFSEIEGFVKKLREGNDLVMGCRLPRGGGKVLPGAMPFSHRWIGNPMFTV
                     MARHMFSSPINDVYCGLRAFTRNLYDRLDLRCQGMEFATEMIIKSSINGAKIAENPIT
                     LHPDGRKAHAPHLKTFRDGWRTLRFFMIFSPRWLFLYPGILLAALGIAGYFIALPGLE
                     IHGIKFDAHTLLFASLAILLGYQSILFAIFAKTFAINEGLLPADAAMKRFFDIVYLER
                     GLLIGFGSFLAGVALLAAAVAQWWSVRFGVLDYSHTMRWVIPGVTLTALGFQTILSSF
                     FVSILGMKRR"
     gene            complement(10211..10828)
                     /locus_tag="VG754_06415"
     CDS             complement(10211..10828)
                     /locus_tag="VG754_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18882.1"
                     /translation="MPMAFNPESRIDALWQEYSEAFSSFDDLTLGRWMAQTLGQLEGR
                     VWRLSHPLLGSYRLAAQLAHQRQVWLKRLVTPPASYAESPCCRAPMLPLLTRDVRDSG
                     LICQHCNETLVPFDEIPAELREPVSAWAERYAPIHAVAHWDDRQRKNAGNYDHAYDQA
                     ADDAERLLAEAARKLMPKFLDFYPAAVWEDQDECLSVHPEDIEGD"
     gene            11054..>11596
                     /locus_tag="VG754_06420"
     CDS             11054..>11596
                     /locus_tag="VG754_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF18883.1"
                     /translation="MMRAAQALFLFSIYLILAGTQASVYADDWYRWRGPNLNGISTET
                     GWQTQWPDEGPKRLWKASVGMGFSSMTVSHGHLYTMGNDGDATDTVYCLDAATGKPVW
                     KHSYPCPLDPHFYEGGTSSTPIVDGDRVYSISRKGNLFCLDAATGKVIWSKNLPEELG
                     CEIPTWGFAGSALVEGDLLIL"
BASE COUNT         2567 a         3049 c         3239 g         2741 t
ORIGIN      
        1 agcggggcga cggcgtaggg cagtcgcaac gtgacgtatc cggcaaattt tgcgcctgcc
       61 ttaaccgcgg cggcaaggat gttgggcatc tcatgatcgg tcaatcccgg cactacgggc
      121 gcgaccatca ctccgaccgg aattcccgcg ttcgcgagcg cttcaatcgc ggcaagccgg
      181 ctggcgggcg gcgatgtgcg cggttccatg actttgcgca aatcggtgtc gagcgtggtg
      241 acggagacga acacgacact acagttgtat cgggcgagtt cactgagcac gtcaatgtcg
      301 cgcgtcacca ggtgattttt tgtcacgatg ccgacgggat tgcggaattc ggccaatact
      361 tgcaggcagc cgcgcgtcag tttcagtttg cgttcaaccg gctggtaaca atcggtcacg
      421 ccgctgatgg aaatggtctt gggtttccat tttggcgatg aaagttcggc gcggagcaat
      481 tcgggcgcgc ggtccttgac catgatgcgc gtctcgaaat cgagtccggc cgaaaacccg
      541 agatattcat gggtgggccg cgcataacaa tagatgcagc cgtgttcgca accgcgatac
      601 ggattgacgc tggcctcgaa accgacgtcc gggctgtcgt tggtggcgat gatggtgctg
      661 ctgtggtcgc ggagaaattg ggtttgcggc aggacgtcct cgtccgggtt ccaatcctcg
      721 tcgcgttcca gatggatttt ttcaaagcgg ttcggcaggt tttccgacgc gccgcgatga
      781 ggaatcacgt ttggtttgct gtccacgcgg ctagaataat acaaggcggc tgaaatgtca
      841 cggaggccga gttaaaatac ccaaatacca aagaaatccc aagctccgag ctttgacaga
      901 atgaacaaaa tgttcagaat ggaatggttt cctaaaatca tattttcatt ccagagccat
      961 ccccgtggga aaatagaatt aggaaagcag gaaatcaaga aaggacgatg tttcctcacg
     1021 aattttgttc ctgatttcat gatttcctaa taaaaaactc tcaatctcat tttctgccgt
     1081 tcatctggtt ttgccaataa aatcgccggt tccgactggc ctgacgccga ttattggatg
     1141 ctactgtttt cagtcttaat attttgttca ttctgggtat ttattcagaa ctcccaatgc
     1201 aatctcgcgc ttaacacatg caccgggccg cgatcgcggt tgaaggcggg gtcgctgacg
     1261 aattggtaat cgagcgtgcc gtggatggtc ttccagatgc cgaagtcgta ataggtttcc
     1321 aattcctttt cgacgccgta attcagcgcg ccgtcgccag ccaggatgcc cgtcccgcca
     1381 atggtgagaa attgctgatg gatatgcgat gcgccgctga aaaccccggc aatggcgaat
     1441 gtgtcgttcg tgcgatgcca gaacgcgccg ttcacgctca agcccaggga agcggctttg
     1501 tccacgtcgc tgtacatcca ggcttcgttg cgtccatcgc tccagccaag ccgcatgaac
     1561 gcgccgatgc ctttggtcaa ttcctgttcc gcattcaggc cgaagccgaa tttgtaacgg
     1621 tattcgcggg tgagtctgat gtcgttgttc aatgcaggat tgtccacgac ttcctcgtag
     1681 ctgcccatgt gcgcgcgatt caggtaggcg agcaatcgga cggcgcccgg atgatcgttg
     1741 aaggcgaagc ggcgttcgaa ttctgtcacc atcgcccaag ccgcagtgta atttttgtcc
     1801 tgcgcaaggc cgttggaaac tcgcgggacc tggaaaaaac cgtaacgcag agttgttttt
     1861 ggttcgttcc attcaattgc gatgccggtg tcatagccga ggctgtccgc cggataatcc
     1921 catgcttcgt ttgcgaccag accccagctc atgaattgcg tgcgcggatc gttggcgtac
     1981 gtgttgttgt cgaaaatgtc cttggcgctg aatttgccga gagtgaaggt gatccgggaa
     2041 atgtcttctt ttccggcgag atgaagcgag tcatcctcga ttttttcctg ttcgccgccc
     2101 aggccgatgg tctggcggag gaacacgcgc gcgaaaacta cgttgggcgc ttgcgtgccg
     2161 acgcgatagg cttcggcatt ggggaaagcc tcaatgccca cggtatggct gaagccgaag
     2221 ccctgccaca tcaaaccgtc cagatgaaat tccgcgccgc gccaaagacg cacgccggtc
     2281 atcaagtcaa gcgagatggt gtcctggact tcaccaacgg ggctgagact gttcggcccg
     2341 ttatattttg tggaaaagcc gggatgacca tcaaggatgt cggtgttttg cgcgtgccag
     2401 ttccaagttt gtaccttcga atcatcggca gcgttggacg cgatgggatt ttcgtcggcc
     2461 atggccagtg agccagcggc actcgttgcc gcgaggattg ccgcgcaaag ttgggattga
     2521 ttccgtttca aacgattcga gatttgtgtt ccggcctttg aaaccaaccg tgccgagtcc
     2581 gaacacggaa acaagaggcg cgatgataga cgaagttctt cgttgggcaa atatattcgt
     2641 ttaaatttgc cgcgttaaaa ggtccaatgc aaccgcgccc cgaaaacatg cacggggcct
     2701 cgatcacgat tgaacgcggg attggaaacg aactggtaat ccaacgagca gtgaattgtt
     2761 ttccaaattt tgaggtcgta gtaagtttcg agcgcctttt cccagccgta ggtcagcgcg
     2821 ccgtcgccat cgagcatgtc cgtgccgcca gcttggagaa atttttggtt gtcgcgcgaa
     2881 gcgccgcttg ccacgaaagc cagcccccaa gtatcgtccg ggcgatccca cgccccgcct
     2941 tttatgctcg cgccgaacga agccgaataa tcaacgtcag tgaacgtcca cgtttcattg
     3001 tggccgtcgg tccagcccaa acgcgaaaac agtccgatgt ttttggatat ttcctgctcc
     3061 caattcaggc caaagccgta tttgtaacga aaggcgcggg ccggtgagat gtccgcgccc
     3121 ggaccattgg ccagcaagat ggatgttgcg ccggcgtagc tggcaaaatt ggcctgatca
     3181 agccaggcca ggaaccggac tgcgccggga tgagcgttga tgctgtacct gcgttcaaat
     3241 tcccccgcca tggcccactc gcgccaaaac gggccatagg ccccgcgatt gggccacatg
     3301 agatattgat cgtcgccggt gaacccattc ttttcgcgcg gcatctggaa gaaagtgtaa
     3361 cgcacggccc attttggctg gttcaattcg acggccatgc cggtggtgta cccgacggtg
     3421 tcctgcccgt aatcgaacgt gttgtttgcc atgaacgccc agttcatgaa ctgcgtatgc
     3481 ggatcgccgg cgtaggcatt gttgtcaatc acgtcaaggg gcgtgaaccg tccgatcgtg
     3541 aagatgagcc gggagatgtc ccgctttcca gcaagtgtga gcggaccatc gggtgtgtct
     3601 tcctgttcgc cgccaaagcc gatggtctgt cgaaggaaca aacgcgcgaa cgtatagttc
     3661 ggactttcgg tcccggcttt gtatgcgtca ccgtttggga aatcctcgat gccaaatgtc
     3721 ttgctcaggc caaagccctg ccagatcaaa aagtccgcgt gcgcctccgc gccgcgccac
     3781 aaccgcgcgc ctgcgaacaa atcgagtgtc actgtttccc gcacctcgcc gccatcgtgc
     3841 aagctgttgg gaccggaata tttcgcggga aaaccgggat cgccctggac tatatccgtg
     3901 ttttgcgcgt gaatgttcca gttttgcgcc tgtgtttcgt tggtggacgc aatgggattt
     3961 tcatccgcga tggccggaga agccgcggcg ctcgttgatg cgaggattgc cgcccaataa
     4021 tgcaatgatg ctttccgatt ccgtttcaaa tgattcgagg tttggtttcc agcgccaccg
     4081 gacgaagtta gacgaataac atttgcatgt caaataatat tagatatgtc taatattggt
     4141 ctagttcaat tgcggcggga ggtgaaagga aaagacccaa ggcgaaagtt aatattacgt
     4201 tacaccgcac ggagattatc ctgcggagat agccaaaaca ggaggggatt gccgttttgg
     4261 agcatcttgg aaggagccga cgtgaggagg tcctgtttgg atttcaaatt tgaaattgga
     4321 aatagggcta tgcacacgat gcccttgccc aaaatccagc aacagcatcg gtttggtggt
     4381 cccaaaagat tcaacaaagc acttttaaac tttaatgttt aattcatact gccgcgtatg
     4441 cgtgacgaat cactggattt cctcaagacc ctggtcaaca cacccagtcc ggtcggccat
     4501 gaagcgcgcg ggcagcgtgt ctggctcgat tacgcgtcga aatttgccga caccacttat
     4561 tccgatgctt acggcaattg cgttgccgtg ctgaacaagg gtggttcgcc gcgtgtcatg
     4621 cttgccgggc atgccgatga aatcgccatg tcagtgaatt acatcgatcc ggaaggtttc
     4681 atttacgtcc gcaaaatggg cggtgtggac gcggccatca ccaaggcaca gcgggtgatc
     4741 attcacacgc gcaatggtgc tgttaatggc gttgtgggaa atgtcgcgcc gcatttgagc
     4801 aagcacgatg acggcgacaa gaagacgccg aagattcatg atctctttat tgatattggc
     4861 gtcagcagca cgaaggacgc cgagaaatta gtgcaaatcg gcgaccccat cacgctgaac
     4921 gacgagtttc aattgttgcg gaatgatttg gccgtggcgc gtgcgttcga caatcgcatt
     4981 ggcactttcg cggttgccga ggcgttgcgt ttgctcaagg aatccaaggg caagctggag
     5041 gcggaagttt gcgccgtctc aaacatccag gaggaagtgg gattgctggg cgcgcggcaa
     5101 atcgcctatt cgctcaagcc cgatgtcgcg attgttgtcg atgtgaccca cgcgacggat
     5161 tatccgaccg tcaacaaagc ccaacacggc aatatcaaag ttggcaacgg cccgaccgtc
     5221 acgcacggcg gttgcaacca tcctgaagtt gtcgcgcgca tcgagcagct tgcgaaaacc
     5281 aaaaaaattc cgttgcagca cgaggcgatg tcctcgacga gcggcacgga tactgacgtg
     5341 atcttctgga cgcgcggcgg catcccgagc gcgttggtca gcctgccgaa ccgttacatg
     5401 cattcgccgg tggaagtggt gagcttgagc gacctcgaaa agattcctga attattggcg
     5461 gcgttcgcca ggtcattgaa aaccggcgaa gaattcaaag tgaagatttg agtgtcactt
     5521 cttcggcggc gcaaagagtt gcagcaagtt ttgtccgttg ctgccagttt gaaaaacgtt
     5581 cgcgccgctc ttgagcaaag cgagcatcag gacggcggaa aaatcctgtt cggatttgat
     5641 gccggtgaaa atctggtggt ccacgttcat cttgaactcg tccaccttgt ccggctgttt
     5701 catgcgcaaa taagtcacct tgccgagagt gagattgagc gtgtcaatgc cggcaaattg
     5761 catccctgac gttttcgatg cggaattttt gttcggactt tttgcggcag gaggaaccac
     5821 ttgcgcctgc gacatgtcaa aatttcttcg tccacttttg tcctccacaa gattgatgcg
     5881 cgaaagattg aaacgcatca gcttgcaatg caatttgcgc gataccaacg cgtcgcgatc
     5941 atactccacg tgcaactccg gcatttcaat aaaaggcgag ccgccgaatg cggccgtgtt
     6001 gtaaagcacc atgttttcaa tcgtcacctg cgggttcaac aaaccgatgc gcaccttctc
     6061 gattttcact tccatgccgg tctggcgatg gatttgatat tccacgtatt cacgggcgaa
     6121 tgtgtccatc agcaggattc cggcgactac gaggatgacc agcagaatca aaaggcgaaa
     6181 tgcccagcgg aaaatccatt tgatcatggg ttctccttcg ttcttccgtt ggcaagaaca
     6241 gttcggttgg gcattgcaca aagcgcaatt cgcatatcag gccgccatgc ttccacaaat
     6301 gaccagcttc ggaaactttt ttcggtctgc cttgatggaa gcgaagctgc atttagattg
     6361 aagccaaatg acggaacgtg taccgtgtat tgttgatgac tttagggtcg ccgatgcttg
     6421 tttggaaact atgcacttca tgcgttctta attgaccgat gtcaaattcc tctcctcaaa
     6481 ttcccgagtt cgatcgtttt gccaccgatt atgatcgcgc cttggccgag ggcatttctg
     6541 tttcgggaga ggacaaagat tactttgcca acgggcgcgt tgaatggctg gcaggctgtt
     6601 tgcacaaact gggctgtcat ccgaaaactg tcctggatta cggttgtggc accggttccg
     6661 ccactccgtt cctggccaga tcgttaagtt ttgactcgtt ggtgggagtg gacgtttccg
     6721 tgcaatcttt ggttgttgcc cgacagcagc acggttcacc ccaggtcaag ttcacaagtg
     6781 tgaacgacta taaacctgcc ggcagcgttg atttggtttt ttgcaacggc gtgtttcatc
     6841 acatcccgcc gaaggaccgg cacgatgctg tgaaattcat ccatgcttcg ctcaaacccg
     6901 gagcattttt ctccttttgg gaaaacaatc cttggaaccc gggcacgcgt tacgtcatga
     6961 gccggattcc ctttgaccgt gatgcaatca cgctgtcgcc gcccgaggcc aggcgattat
     7021 tgcgcgcggg gggctttgaa attgtccgca ctgacttttt attttggttc ccccgatttc
     7081 taagctggtt tcgacgactg gaaaaactgc tctcgccctt gccgatggga gcacaatatc
     7141 atgtgctttg ccggaaaccg cacaatcgcg gttgattccc gtcgattgtt gacaacaggg
     7201 attgtgaact cgcgctttcg cttgacccag cctcccgcca caggttgaat tttataatga
     7261 acagcaagag cgagcgggtg caatccagcg tcacagcatc ctcgaaagcc atcatcattt
     7321 ttctgctcat tttggctgct gcgcttcgca tctggaatct gcctgctctc aatgaagtgc
     7381 gcgattggga cgaaattggt tacacgaccg acggattgat ggcgtgggaa gggcttctac
     7441 cggggtggga aagtgtgccg gcaggaccgc aaacttggga gagctggctt ttcgtgggcg
     7501 cgcgctcggg ttgggaattt ctgcattcgc aacgcaaagc ggcaaccccg ggcgcgttga
     7561 aacccttgat ggcgattgac caggcgctgt tcaaaactta cgaagacttg ggtccgttga
     7621 ggcgattgtt attgtggatt tcaatcgtca tctcattggc aggcgtttat ggcgcatatc
     7681 ggcttggggc aaaatacggt ggcgtagcgg gaggattgct catgggcggg ctggcagcgg
     7741 tgttgccgtt gtatctggaa tatgcgggtg ttgcgaaatc ttgcagcgat gcgtggatgt
     7801 tctcaatttt ggcggtatct tgcgccgcaa cgatgacggg agcgaaacga tgctggtggg
     7861 ccgggaccat gctgggattg gcgatcggtt cgcgggttga catggcagcg acggtgccgc
     7921 tggtgttttg gggattatgg gaaagtcacg agaaggattc gttgtggaaa agcatggctg
     7981 gcacaattgc tctggcaatg gccattgcac tgttgtcggc gccattcgcc atgcagggat
     8041 tcgtaggaat tttaaggcaa atcgttctca gcggcagcgg gacgtactgg ggcgcagaac
     8101 ctcaaagatt gaggaccttg aaggagttgg cttgggacca ggggttggga ctaattttta
     8161 taatagctgt cgtcggcctg ttctttttgc cttacggaac gagattgaaa cgcggcacgt
     8221 tggctgcatt tgccgcattg gtggtggttt ccatttttgc gaggcaccat cagccagtac
     8281 ggtactacgg cgctcagatt attgtcatcc ttgtgttcat cgccgtggga gcaggcgcgc
     8341 tgttccgacg gtggcccaaa ccggcactcg cgtttgcaat gttgctgctg atcttccccc
     8401 tggtgcaatc gattcgggca gcaatcgcca tgaaggcgat tcatgtgccg gagacttcga
     8461 cggaatgggt ggatgcgcat gtgccggcgg gaacgaccgt gtatgtgcat cccgcgttca
     8521 tttcacgtgc agtattaccg acggaagaag cggctgatgc catctgggac attgtgaccg
     8581 gcaatcaggc gtggcgcacg aaagttctgg acggctttca ccggtttgcc atacctgaca
     8641 ggcaattgcc aagggccctt tcggaggata atctttgcaa agatcggggt gtttgccggc
     8701 gatggttcat tctcggcggt ggacagagca accgccccag gtacaatctg caattggttc
     8761 cgatgtccgc aacttttggc ctgcaacggg aaactttagg gccagaattt cagagaacgg
     8821 gaggtgtggt gatctggccg acacgcgcca gcggtttgcc cggtggattg ggcgaacctt
     8881 ttataaaatg ggtgaactac aacggagatg gaatcttggt gtttgtctcg ccggatgtgc
     8941 gggaaaaact cacccattaa gcgacttttt attctaccgc cgtttcatcc cgagaatgct
     9001 gacaaaaaaa ctggaaagga tggtctgaaa tcccaatgcc gtgagagtga cgccgggaat
     9061 gacccagcgc atggtatggc tgtaatcaag gacgccgaac cgcaccgacc accattgagc
     9121 gacggccgcc gcaagcagcg ccacgcccgc cagaaacgaa ccaaatccga tcaacaatcc
     9181 gcgctccaga tacacgatgt caaaaaaacg tttcatcgcc gcatccgcgg gaagcaagcc
     9241 ttcattgatc gcaaaagttt tcgcaaaaat ggcaaacaag attgattgat agccaagcaa
     9301 aatcgcgagg ctcgcaaaga gcagcgtatg cgcgtcaaat ttaatcccgt gaatttccaa
     9361 tcccggcaac gctatgaaat atcccgcaat tcccagcgcc gccagcagga ttcccggata
     9421 aagaaacaac cagcgcggac tgaagatcat gaaaaatctc agcgtccgcc agccgtcgcg
     9481 gaacgttttc agatgcggcg catgggcttt gcgcccatcc gggtgaagtg tgatcgggtt
     9541 ctcggcaatt tttgcgccat taatgctgga cttgatgatc atctcggtgg cgaactccat
     9601 cccctgacag cgcaggtcga gccggtcgta caaatttctc gtgaacgccc gcaatccgca
     9661 ataaacgtca ttgatcgggg aggaaaacat gtgtcgtgcc atcaccgtga acatcgggtt
     9721 tccaatccaa cgatgcgaaa acggcatcgc gcccggcaac accttgccgc ctccgcgcgg
     9781 caagcggcaa cccatgacca ggtcattgcc ttcgcgcaat tttttgacga acccttcgat
     9841 ttccgagaag tcgtaactgt catcggcgtc gcccatgatg atccaccggg cttgcgcggc
     9901 ttcgatgcct cccttcaagg cgttgccata gcccttttcc ttcacatgaa cgacgcgcgc
     9961 gccgattttt tcggcaatct gcaccgaacc atcggtgctg ccattgtccg caatcaaaat
    10021 ctcgccgcgc acgcccgcgc gtttcaagcc gagatgcgct ttctcgatgc aggttgccag
    10081 cgtgtccgct tcattcaaac acggcatcac aatcgttatg tcataagcat cggacatgaa
    10141 tatgagttga taagcgtatc gctccgacgg tttcagtggc aaattgtttg ttgtcgagcg
    10201 ttgtttcgac tcagtcacct tcgatgtctt ccggatgcac cgagaggcat tcgtcctggt
    10261 cttcccagac tgccgcggga tagaaatcca aaaatttcgg catgagcttt cgcgctgcct
    10321 cagccagcaa ccgttcggcg tcatcggcag cctggtcata agcatgatcg taattgcccg
    10381 cgttcttgcg ctgacggtcg tcccaatgcg ccaccgcatg gatcggcgcg taacgctccg
    10441 cccacgcgct gaccggttcc cgcaattccg ccgggatttc atcgaacggc accagcgtct
    10501 cgttgcaatg ctggcagatc aaacccgagt cccgcacatc gcgcgtcaac agcggcagca
    10561 tcggcgcgcg gcagcaggga gattccgcgt agcttgccgg aggggtcaca aggcgcttca
    10621 accaaacctg cctttgatgc gccagttgcg cggcgagacg gtacgaaccg agcaacggat
    10681 gcgacagccg ccaaacgcgt ccttcaagct ggcccaacgt ctgtgccatc catcgcccca
    10741 gagtaaggtc atcaaaactg ctgaacgcct cgctgtattc ctgccacaac gcgtcaatcc
    10801 gtgattcggg gttaaatgcc atcggcatta gtttgaactt gagcggctga tttgtcgagg
    10861 cgagagcgtg tgtaaatccc aaagcctcaa ataccgaatg aagcaccaga gaacaatcaa
    10921 tattcaaact ccaatccgcg ccgcaagatt tgtggcgcgg ggctttagat ttaatcattc
    10981 cttggggatt tcgacattgg aaatttttac ttccattgac actcaatgtc agttgaggta
    11041 cttctgctcc atcatgatgc gcgccgctca agccctcttt ctcttctcca tttatctgat
    11101 acttgccgga acgcaagcga gcgtttacgc ggatgattgg tatcgctggc gcgggccaaa
    11161 cttgaatggc atttccactg aaacgggctg gcaaacccaa tggccggacg aaggtcccaa
    11221 aaggctttgg aaagcatccg ttggaatggg cttctcctcc atgaccgtca gccatggcca
    11281 tctttatacc atgggcaatg atggcgacgc gacggacacg gtgtattgcc ttgatgccgc
    11341 gacaggcaag ccggtatgga aacattccta tccttgcccg cttgatccgc atttttacga
    11401 aggcggcacc agcagcacgc ccatagtgga cggtgatcgc gtctatagca tcagtcgtaa
    11461 agggaatctc ttctgtctcg acgccgcgac aggcaaagtg atctggtcca aaaatcttcc
    11521 cgaagagttg ggttgcgaaa ttcctacgtg gggtttcgcc ggttcggcat tggtcgaagg
    11581 cgatttgctg attctg
//
LOCUS       DASZMM010000266         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1224313, whole genome shotgun sequence.
ACCESSION   DASZMM010000266 DASZMM010000000
VERSION     DASZMM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224313"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VG754_06425"
     CDS             <1..309
                     /locus_tag="VG754_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18884.1"
                     /translation="GLLAAIAIPNFVHARTTAQQNACINNLRQIDGAKQEWALENKAT
                     ATAVPTSANIQPYMGRGSAGSLPVCPLDSSATFTTSYSIQDLSTAPTCNISSTNHILN
                     "
     gene            409..1209
                     /locus_tag="VG754_06430"
     CDS             409..1209
                     /locus_tag="VG754_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF18885.1"
                     /translation="MSNKLLSRDVSVERMKPIIEIDNLRVEYRTRAIGQTTKVAVDGL
                     SLNVLQGEVFGFLGPNGAGKTTTMNVLLGFVNATSGMARLFGVDVRQPIARQRIGYLP
                     ELTYYYKFLTAEELLRFYAQIFGISRRDADRRIDELLKLVELESARKRPIKSYSKGMQ
                     QRVGLAQALINNPDLLILDEPTSGLDPIGRMKVREIIQRLKNEGKTVLFSSHELGEVE
                     TVCDRVAILHQGKLQVEGRVNDLLNKYQSNLEQIFLRIVGYPSQPAAA"
     gene            1206..1985
                     /locus_tag="VG754_06435"
     CDS             1206..1985
                     /locus_tag="VG754_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWF18886.1"
                     /translation="MNAIFALAKVVIKELYRRKDFYVLFILTVLITLTMASANLFHDD
                     RITGYLKEICLRLIWVSALVVAVGTAARQIPAERENRTIFPLLAKPVTRAHVVAGKFL
                     GCWLACGLALVVFYLFLGVVSASHEHEFRLINYFQALWMHWMFLAVVIAMVLLGSIIF
                     AAPSSNATIVIILVLGILLVGQHLGRLAIHQPEPQRTILYALYFIIPHLEFFDLQDFV
                     VFKIGWVDWKYLALASLYALAYTAFFLCATWVCFRRKPLSQ"
     gene            1991..2869
                     /locus_tag="VG754_06440"
     CDS             1991..2869
                     /locus_tag="VG754_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18887.1"
                     /translation="MPSPALILTFLMVLSFSLATWIQPRAAGLGKRGQSDDVLKIILG
                     DARRMFANHFFTKADVYFHSGYYPSFFEQAYQQQAVDTKHLTEVHDHHDGHEDHDEDE
                     HERAMDFLGKPHDWIDGFGRHFYSSHHSHLDKPGEAREILPWLRLSAELDPQRIETYT
                     VAAYWLREHLNKVDDAEAFLREGLQANPSSFEILFDLGKLYYENRHNPTLARNLWELA
                     LRRWIEQDDAGKEPDVAAYGAIVANLSHLEEEQGNLREALEYKEMELEVSPVPETIQQ
                     QVDELKQKLSASAARK"
     gene            complement(2947..>3322)
                     /locus_tag="VG754_06445"
     CDS             complement(2947..>3322)
                     /locus_tag="VG754_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HWF18888.1"
                     /translation="KAAQMKPITVLNEAELEETFSRASGPGGQNVNKVSTRVTLRHLP
                     TNISVTVQDSRSQAMNRQLARERLLAALQQRERQAAAAARHQREQLRRQTAKRPRVAK
                     ERMLAAKHHRAAVKKERRFRDE"
BASE COUNT          715 a          900 c          889 g          818 t
ORIGIN      
        1 ggtcttttgg ccgccatcgc gatccctaac ttcgtgcatg cccgcacgac agcgcagcaa
       61 aatgcttgca tcaacaatct tcgtcagatt gacggtgcca agcaggaatg ggctttggaa
      121 aacaaagcca ctgcgactgc ggttcccacc agtgcgaaca ttcagcccta catgggccgt
      181 ggttccgctg gttcgctgcc ggtctgcccg ctcgattcga gcgcgacgtt cacgaccagc
      241 tacagcatcc aggacctctc caccgctccg acttgcaaca tttcgagcac gaatcacatc
      301 ctgaactaat cggcaattcg aagtgtagtt tcaaaaggct ttggagtcat ttccaaggcc
      361 ttttttgttt tccgggcgca atggcaaaaa aaggttgttt cgtatccagt gtcaaataaa
      421 ctcttaagtc gagacgtcag tgtcgagcgg atgaagccga taattgaaat tgataatttg
      481 cgcgtggaat atcgcacccg cgccatcggg cagaccacga aggtggctgt ggatggcttg
      541 agccttaatg tgctgcaggg tgaggtgttt ggtttccttg ggccgaacgg cgcgggcaaa
      601 accaccacaa tgaacgtgct gctcggtttt gtgaatgcaa ccagcggcat ggcgcggctc
      661 tttggcgttg acgtgcgcca gcccattgcc cggcaacgca tcggctatct gccggaattg
      721 acttattatt acaagtttct tacggcggaa gaattgttgc ggttttatgc gcagattttc
      781 ggaatctccc ggcgtgatgc cgaccgccgc attgacgaac tgctcaagtt ggtggaactg
      841 gaatcagccc gcaaaaggcc gatcaaaagt tactcaaaag gaatgcagca acgggtcggc
      901 ctggcgcagg cgctcatcaa taatcccgat ttgcttattc tcgacgagcc gaccagcgga
      961 ctggacccca tcggtcgcat gaaagtccgc gaaatcatcc aacgcctgaa aaacgaggga
     1021 aaaaccgtgc tcttttcttc acacgaactg ggcgaggtag aaacggtctg cgatcgtgtg
     1081 gcgatacttc atcaaggaaa acttcaagtg gaaggccgcg tgaacgacct cttgaacaag
     1141 tatcaatcaa acttggagca gattttcctc cgcatcgtcg gttatccttc ccaaccagcc
     1201 gcggcatgaa cgctattttc gccctcgcca aggttgtcat caaggaatta taccgtcgca
     1261 aggattttta cgtcctgttc attctgacgg tcctcatcac gctgacaatg gcatccgcca
     1321 atttgttcca tgacgatcgc atcaccggct atttgaagga aatctgtctg cggcttatat
     1381 gggtttcagc acttgtcgtg gcggtcggga cggccgcgcg ccagatccca gcggaacggg
     1441 aaaaccggac catctttcca ttgcttgcca agcctgtgac ccgcgcccat gtcgtggcgg
     1501 gaaaattcct cggctgctgg ctggcctgtg gtttggcgct ggttgtgttt tatctgtttc
     1561 tcggtgttgt cagcgcgtcg catgagcacg agtttcgcct gatcaattat ttccaggcgc
     1621 tctggatgca ctggatgttc ctggcggtgg tcatcgcgat ggtgctgctc ggctccatca
     1681 ttttcgccgc gccttcctcc aatgcaacga tagtcatcat cttggtcttg ggaatcctgc
     1741 tggtgggaca acacctcgga aggttggcca ttcatcaacc ggaaccgcaa cggaccattt
     1801 tatacgcgct ctatttcatc attcctcacc ttgaattttt cgatcttcag gatttcgtcg
     1861 ttttcaagat tggctgggtg gactggaaat atttggcgct ggcatcatta tacgctttgg
     1921 cctatacggc gttctttctt tgcgcaacct gggtttgttt tcgccgcaaa ccattgagcc
     1981 aataacatcc atgccatcac ccgcgcttat cctgacgttt ttgatggtgc tttccttcag
     2041 cctcgcgaca tggatccagc cgcgcgcggc gggcctggga aagcgcggac aatccgacga
     2101 cgtcttgaaa atcatcctcg gcgatgcgcg gcggatgttc gcgaaccatt ttttcacgaa
     2161 agccgatgtt tattttcaca gcggttatta cccttccttt ttcgaacaag cttaccaaca
     2221 gcaggcggtt gataccaagc acctgaccga ggtgcatgat catcacgacg gtcatgaaga
     2281 ccatgacgag gatgaacacg aaagggcgat ggattttctc gggaagcctc atgattggat
     2341 tgacggtttt ggccggcatt tctattcctc gcatcattcg catctcgaca agcccggcga
     2401 agcgcgcgaa atcctgccat ggcttcgcct ttccgcggaa ctggaccctc aacgcatcga
     2461 aacttacacc gtggcggcgt attggctccg cgaacatttg aacaaagtcg atgatgcgga
     2521 ggcgttcttg cgcgaggggt tgcaggcaaa tccatcgagt tttgaaatcc tgttcgactt
     2581 gggaaaatta tattacgaga accggcacaa cccaactctc gcgcgcaatc tttgggaact
     2641 cgcgttgcgc cgctggattg aacaagacga tgctggaaag gagccggacg tcgcggccta
     2701 cggcgcgatt gtggcaaacc tttcgcattt ggaggaggag caggggaacc ttcgtgaagc
     2761 tttggaatac aaggaaatgg aattggaagt ttcgccggtg cccgaaacaa tccagcagca
     2821 ggttgacgag ttgaagcaga aactctccgc ttcggcagcg cgcaagtaag ttcaaactct
     2881 attgaattac aggcgcgctc aatattgctc atcactcctg tactccatca ctccattccc
     2941 tcgatctcac tcatctctga atcgccgctc cttcttgacc gcagcgcgat gatgtttggc
     3001 ggccagcatc cgttctttgg caacacgcgg acgtttggcc gtctgtcggc gcaattgctc
     3061 gcgttgatgt cgggcagcgg cggcggcctg gcgttcgcgt tgttgcaatg cagccagcaa
     3121 tcgctcgcgg gcaagctgtc ggttcatcgc ctgtgaacgc gaatcctgca cggtgacgct
     3181 gatgttggtc ggcaaatggc ggagcgtgac gcgggtggaa accttgttga cgttctgacc
     3241 gcccgggccg gaggcgcggc tgaaagtttc ctccaactca gcttcattca ggacagtgat
     3301 cggtttcatt tgtgccgctt tc
//
LOCUS       DASZMM010000267         6772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3954805, whole genome shotgun sequence.
ACCESSION   DASZMM010000267 DASZMM010000000
VERSION     DASZMM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3954805"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1267
                     /locus_tag="VG754_06450"
     CDS             167..1267
                     /locus_tag="VG754_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HWF18889.1"
                     /translation="MSLLALHELHAGLNGRFADVNGCEVTADYGDWLAEHAALRESAG
                     VLDLSFRGRICLTGADRARWLNGQVTNNVKDLREGQGCYAALVNAKGKLQSDLNIFCL
                     KDELLLDFEPGFTAAVSQRLEKYIIADDVQVVDVAPHYGLLSVQGPKAETIVKRLELF
                     KEIPAQPLTFVSFKDATLGEIYLMNQPRLATIGFDLFVPTPALGAVLDKLIAAAREIG
                     GRVCGWSAFETARIEAGVPRFGIDMDENNLAPETGIEGRAISYSKGCYIGQEVIARIR
                     TYGQVAKSLRGLRLSDDLKTLPAKGDKLFHDGKEVGFITSAVHSPSLRANIAIGYVRK
                     ESNTLGTQLKLHTAQGESAAEIVPIPFVGSQS"
     gene            1382..4078
                     /locus_tag="VG754_06455"
     CDS             1382..4078
                     /locus_tag="VG754_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18890.1"
                     /translation="MKIEKKPSNSGTAKAADPFGQKSPLFWATLLLLGLLAVIFWRCF
                     IPSYVLFSNDGPYGAMVQEYNRMPSIIHGVWANMTWFGNESVSPPPSVSSLMRILTTP
                     LTPLIYAKILPPVSLLIAGIGACFCFRKLRLAPLACILGGIAAALNSDFFSTACWGVA
                     TQVIGFGFMYIAVGLIVDPSVKRQWVRAILAGLAVGVGVMEAYDIGALFSLFIAAFVV
                     YHAFFLSERPASTAQKGAQGIIKVGLVGGFALFIAAHSLLSLVNTQIQGISGAQQDEA
                     TRQARWDEATQWSLPKAETLQVFIPGVFGFRDSWYMYEYDEPTNDQYWGTIGNGGGLN
                     HLIGTGFYAGVPVVVIALWAVLQALRRQGSPFTIFQRRAIWFWLGTTIVSVLMSYGKH
                     APFYRFFYALPYASTIRNPDKFMHVASWALVILFAYGVHGLVTVYLENYVARVGGFFG
                     QFKTWLAKASSFERKWWYGSFIAIGLSLVGWLMYASMGSKLMAYVQTVGVPATDARGV
                     VNFSFQAVAWFVLFLVVTVVLLTLIFSGQFSGSRARWAGLLLGALMVFDLGRADVPWI
                     VYWDLDYKYAPDPIIQILADKPYEHRIALLPRPPDNPDQETAQQFALLVNAYNSDWKQ
                     HLFPFFNIQCAEVVQEPRMPLDKQMYLSYFPPNNPVRFWQFSNCRYMLGATDLIRQLD
                     PQQRMFRVLKTFNFVPKRDNPSTWPADWRTELNPNGKLALIEFLGALPRAKLYSHWQV
                     NTNDQAILQTIANPAFDLFHDVVVANDIPAPSTTNADPGTVEINPNYKSKRIEMQADV
                     KVPSVLLFSERYNPKWQVEVDGKPGPLLRCDFIERGVYLTPGKHDVVMRFAPPITTLW
                     ISLAAIAVGLGLWGFLIFSPAEPEELAEEKKDTQAKEKAAAK"
     gene            complement(4095..5270)
                     /locus_tag="VG754_06460"
     CDS             complement(4095..5270)
                     /locus_tag="VG754_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF18891.1"
                     /translation="MSTATVSESNRVGSAPSSTPDFSKPVQKPRKSTLKLIGEVLNHG
                     GPGYLQFAITNICNAKCDFCGFAVDRFDPKQRRSVTLKEAKDVIDIAVKNHIGYLLFV
                     GGEPLVHKELRAMVRYCAESGIHPMICTNGSLWSDQNMRDLANDGLSSVIMSIDSHDV
                     TKHEKNRGLPDVCRKIQRANEVFSQLGIQTTASVTASKLIDDYEKLPDFLKTLGFKSC
                     TFSYPLTSLASSYLSFSDSNLVNYKTDELIGVFEKIKQMKARSGFPVVNPAESLTEMQ
                     RHLRKEPEKFGCLGGHKYFYLDWNLNLYRCHAWEMPMCNIYEFDQSKLIRDGCTRCMI
                     DCYRDPSVMQFVAINASDAYQNLKRGKFVAAAKNIFDRRNLTSLKAVWDDRKWIGEV"
     gene            complement(5267..5551)
                     /locus_tag="VG754_06465"
     CDS             complement(5267..5551)
                     /locus_tag="VG754_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF18892.1"
                     /translation="MFPLTAYIEAALELARYDKLEDGSFAGEIPKLKGIAAFGSSLRE
                     CEKELRSTLEDWILVGLRLGHKLPVLAGIDLNKTSHGRMGSTQKACAFIS"
     gene            complement(5595..>6772)
                     /gene="hpnJ"
                     /locus_tag="VG754_06470"
     CDS             complement(5595..>6772)
                     /gene="hpnJ"
                     /locus_tag="VG754_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HWF18893.1"
                     /translation="IKAQKPGTAIGFVGAHAAVLPTETLKASPAIDWVGRKEFDFTCK
                     DVAEGRPLSEINGLSYRKDGKIVHNPERELIQDMDALPWVTDVYKRDLQIEKYSIGYL
                     KDPYVSLYTGRGCPAQCTFCLWPQTIGGHKYRVRSAENVAAEMAHAKKLFPQVQEFFF
                     DDDTFTANLPRAREIATQLKPLGLTWSCNSRANVNYETIKHMKDCGLRLFLVGYESGN
                     QEILNRIKKGIQLEEAKLFTKNCKELGVVIHGTFILGLPVETKETIEETIRYAMELDV
                     FSIQVSLAAPYPGTELYEMARQNGWFAKKDKTDIIHDDGLQQSTLEYPGLSKEEIFEA
                     VDRFYRTYYLRPKPILRIIKTMLEDKDVCVRRLREGYEFFRTLNQRKKDLAAATSAA"
BASE COUNT         1426 a         1803 c         1899 g         1644 t
ORIGIN      
        1 cgttggccac ttgggtatcc taccgaccgg cctgccgcat catgccacca taacgcgggt
       61 tgcagattaa gccaaactcg aaccgccttg tcaacggtgc gcaagctgcg cccgaattac
      121 tcaaatttgc tttgtctccg ggcggaatgt tggcgaaatt atctccatga gtttgctggc
      181 gttgcacgaa ttgcatgcag gattgaacgg ccggttcgcg gatgtgaacg gttgcgaagt
      241 aaccgccgat tatggcgatt ggctggcgga acacgccgcc ttgcgtgaga gcgcgggcgt
      301 gctcgatttg agttttcgcg gccggatttg cctcacaggc gcagatcgcg cgcgctggct
      361 gaatggccag gtgacgaaca atgtgaagga tttgcgcgaa ggccagggct gctacgccgc
      421 actcgtgaat gcaaaaggca aattgcaaag cgacctgaac attttttgcc tcaaagacga
      481 actcctgctc gatttcgaac cgggcttcac cgcagccgtc tcacaacggc tggagaaata
      541 catcatcgcc gatgacgtgc aggtcgtgga tgtcgcgccg cattacggat tgttgagcgt
      601 gcaagggcca aaggcggaaa ccatcgtgaa gcgattggaa ctgttcaagg aaattcctgc
      661 acaaccgctg acttttgtca gcttcaaaga cgcgacgctt ggtgaaattt acctgatgaa
      721 ccagccgcgg ctcgcgacaa tcggattcga tctgtttgtt cccacgcccg cgctgggcgc
      781 ggtgttggac aaattgatcg ctgctgcgcg cgaaattggc ggacgggttt gtggctggtc
      841 tgcatttgaa accgcgcgca tcgaggcagg cgtcccgcga ttcggcatcg acatggacga
      901 aaataatctc gcgccggaaa ccggcatcga ggggcgggcc atcagttatt ccaaagggtg
      961 ctacatcggc caggaagtca tcgcgcggat acgcacttat gggcaggtgg ccaaatcctt
     1021 gcgcggcttg cggctgtcgg atgatttgaa aactttgccc gcaaagggag acaagttgtt
     1081 tcacgacggg aaggaagttg gtttcatcac aagcgcggtg cattcgccgt cacttcgtgc
     1141 gaacatcgcc atcggttatg tccggaagga atccaacacg ttgggaacac aattgaaatt
     1201 gcacacggcc caaggcgaaa gcgcggccga aattgtgccg ataccatttg ttggctctca
     1261 gtcataaaat cgtgggccaa ccgggaccaa tccatttgcg atagcgcctg ggtgctccca
     1321 cacggataat tgtcattgct ccgtcagatg atgcggttat ggtatctttc gataaacact
     1381 gatgaagatt gaaaaaaagc caagtaattc cgggacggcc aaagccgccg atccctttgg
     1441 tcagaaaagt ccgttgttct gggccacact gttgttgttg ggattgctgg cggtgatttt
     1501 ctggcgctgc ttcattccgt cttacgtcct gttttccaac gacggccctt acggcgcgat
     1561 ggtccaggaa tacaaccgga tgccctcgat cattcacggt gtctgggcaa acatgacctg
     1621 gtttggcaat gaatccgtga gtccgccgcc gtcggtttcc tccctgatgc ggattttgac
     1681 gacaccgttg acaccgttga tttacgcaaa aattcttcca cccgtttcgt tgctgatcgc
     1741 gggaattggc gcttgctttt gtttccgcaa acttcgtctt gcgcctttgg cgtgcatctt
     1801 gggtggaatt gccgcggccc tcaactcgga ctttttctca actgcatgct ggggtgtggc
     1861 gacccaggtc atcggctttg gattcatgta tattgcggtc ggattgatcg ttgacccttc
     1921 cgtaaaacgg cagtgggtgc gcgcgatcct tgctggtctg gcagtcggag tcggagtgat
     1981 ggaggcttat gacatcgggg cgctgttcag tttgttcatc gcggcattcg tggtctatca
     2041 cgcgttcttt ttaagtgaac gtcccgcttc cactgcgcaa aagggcgctc aaggcattat
     2101 caaagtagga ttggtcgggg ggttcgcttt gttcatcgcc gcgcattctc ttctcagcct
     2161 ggtcaatacg cagatccagg gaatatccgg agcgcaacag gatgaggcaa cccggcaggc
     2221 gcgatgggat gaagcgactc aatggagtct tcccaaggcg gaaacgttgc aggtgttcat
     2281 cccgggcgtt ttcgggtttc gcgacagttg gtacatgtat gaatacgatg agccgacgaa
     2341 cgaccaatac tggggcacca tcggcaacgg cggcggatta aatcacctga tcggcaccgg
     2401 attttatgcg ggcgtgccgg tggtggtgat cgcactgtgg gcggtgttgc aggcgcttcg
     2461 acggcaaggc tctcctttca cgatcttcca aagacgggcg atttggtttt ggcttgggac
     2521 gacaatcgtg agcgtgctga tgtcttacgg caagcatgcg cctttctacc ggttttttta
     2581 tgcgctgcct tacgcctcga ccattcgtaa tccggacaaa ttcatgcacg tcgcgagctg
     2641 ggcattggtg atcctgtttg cctacggtgt gcatggtctg gtgacggttt atctggaaaa
     2701 ttatgtggcg cgcgtcggtg gattttttgg tcagttcaaa acttggctcg ccaaggcttc
     2761 gtcctttgag cgcaaatggt ggtatggctc cttcatcgcg atcggcctca gcctggtcgg
     2821 ctggttgatg tatgcctcga tgggttccaa actgatggcg tatgtgcaaa ctgtcggtgt
     2881 cccggccacg gatgcgcgcg gtgtggtgaa tttcagtttc caagcggttg cgtggtttgt
     2941 gcttttcctg gtggtgacgg tggtattgtt gacattgatt ttcagcggcc aattttccgg
     3001 ttcacgcgcc aggtgggccg gattgttgct cggcgctttg atggtctttg atttgggacg
     3061 cgccgacgtg ccatggattg tttattggga cttggattac aaatacgccc ccgatcccat
     3121 catccaaatc ctggcggaca aaccgtatga acatcgcatc gcactgctgc caaggcctcc
     3181 cgataacccc gaccaggaaa ccgcccagca attcgcgctg ctcgtcaacg catataacag
     3241 tgattggaag cagcacttgt ttccgttctt taacatccag tgcgcggaag ttgtgcagga
     3301 gcctcgcatg cctttggaca agcaaatgta tctgagctac tttccgccaa acaatcctgt
     3361 ccgtttttgg caattcagca actgccgcta catgctgggc gcgacggatt tgatcaggca
     3421 gctcgatccg caacaaagga tgtttcgcgt gttgaagact ttcaacttcg tgcccaagcg
     3481 cgacaatccc agcacatggc cggcggattg gcgcacggaa ctcaatccga acggtaaact
     3541 cgcccttatc gaatttcttg gcgcattgcc gcgcgccaag ctttattcgc attggcaggt
     3601 caacacaaac gatcaggcca tcttgcaaac catcgccaat ccggcgttcg acctttttca
     3661 tgacgtcgtg gtcgcgaatg acatccctgc accgtccacc accaacgcgg accccggcac
     3721 ggtcgaaatc aatcccaact acaaatccaa gcgcatcgaa atgcaggcgg atgtgaaagt
     3781 cccttcggta ttgctgttca gcgagcggta taatcccaag tggcaggtcg aagtggacgg
     3841 caaacccggt cctctcctgc gctgcgattt catcgagcgc ggtgtttatc tcacgccggg
     3901 caagcacgat gtggtcatgc gttttgctcc gcccatcacc acgctttgga tcagtcttgc
     3961 ggccattgcg gtcggattgg ggttatgggg atttctcatc ttctcccctg cagaaccgga
     4021 ggaattggcg gaggagaaaa aagacacgca ggcgaaggaa aaagccgctg cgaagtgaac
     4081 ggaaggaact ccgttcagac ttcgccaatc cacttgcggt catcccaaac ggcctttaat
     4141 gaagtgagat tgcgccggtc aaaaatattt tttgcggcag cgacaaattt gccgcgcttc
     4201 aaattttgat aggcgtcgct ggcgttgatg gcgacgaatt gcatcacgct cgggtcgcgg
     4261 taacaatcaa tcatgcagcg cgtgcaaccg tcgcggatga gcttggattg gtcgaactcg
     4321 tagatgttgc acatcggcat ttcccacgcg tggcaacggt agaggttcaa gttccaatcg
     4381 aggtaaaaat atttgtggcc gccaaggcag ccgaattttt ccggttcctt gcgcaaatgc
     4441 cgctgcattt ccgtcagtga ttcggcggga ttcacgacgg gaaaaccgct gcgggccttc
     4501 atctgcttga ttttctcgaa cacgccgatc aactcatccg tcttgtaatt gacgagattt
     4561 gaatcgctga agctcagata gcttgatgcg agcgaggtta gcggataact gaatgtgcag
     4621 cttttgaagc cgagcgtttt caggaaatcg ggcagctttt cgtagtcatc aatcaacttg
     4681 ctcgcggtga cgctcgcagt ggtctgaatg ccgagttggg aaaacacttc gttggcgcgc
     4741 tggatctttc ggcaaacatc cggcagcccg cggttcttct cgtgcttggt gacgtcgtgc
     4801 gaatcgatgg acatgatgac gctgctcaag ccgtcattcg caaggtcgcg catgttctgg
     4861 tcgctccaga gcgaaccgtt ggtgcaaatc atcggatgaa tgcccgattc ggcgcagtaa
     4921 cgcaccatcg cgcgcagttc cttgtggacg agcggttcgc cgccgacaaa gagcaggtaa
     4981 ccgatgtggt tcttcaccgc gatgtcgatg acatccttcg cttccttgag cgtgacactg
     5041 cggcgctgct tcggatcaaa acggtccacg gcaaaaccgc aaaagtcgca cttcgcgttg
     5101 cagatgttcg tgattgcgaa ttgcagataa cccggcccgc catgattgag cacttcgccg
     5161 ataagcttca acgtgctctt gcgcggcttc tgcaccggtt tggaaaaatc gggcgtcgag
     5221 gaaggcgcgg agccgacacg gtttgattca gaaacggttg cggtgctcat gaaataaatg
     5281 cgcacgcctt ttgagtggag cccatcctgc catggcttgt cttgttcaaa tcgatccctg
     5341 ccagcacggg cagtttgtgg ccgagcctca acccgacgag tatccagtct tccaaggtgg
     5401 atcgcagttc cttttcgcac tcacgcagcg aggagccgaa agcggcaatg cccttcaact
     5461 taggaatctc gcccgcaaat gacccgtctt ccagcttgtc atagcgggcc agttccagcg
     5521 cggcctcaat ataagcagtc aagggaaaca ttatttttca aaatcctcat ccagatccaa
     5581 atcgccagtt cattctaagc cgccgaagtc gccgccgcga ggtctttctt ccgttgattg
     5641 agcgttcgga aaaattcata gccttcgcgc aaccggcgca cgcagacatc cttgtcctca
     5701 agcatcgtct tgatgatgcg caaaatcggc ttgggccgca gataataggt gcgataaaaa
     5761 cggtccaccg cctcaaaaat ttcttccttc gagaggccgg gatattccaa cgtggattgc
     5821 tgcaagccgt catcgtggat gatgtcggtc ttgtccttct tggcaaacca gccgttctgg
     5881 cgggccatct cgtaaagctc cgttccggga tagggtgcgg cgagggaaac ttggatgcta
     5941 aacacgtcca gctccatcgc gtaacggatt gtttcctcga tggtctcctt cgtctcgacc
     6001 ggcaaaccaa ggatgaatgt gccgtggatg acgacgccga gttccttgca attttttgtg
     6061 aacagctttg cttcttcgag ctgaatgcct ttcttgatgc gattcaggat ttcctggttg
     6121 cccgattcat agccaacgag gaacaggcgc aggccgcaat ccttcatgtg cttgatggtt
     6181 tcgtaattca cgttcgcgcg gctgttgcac gaccaagtga ggccgagcgg cttcaattgc
     6241 gtggcgattt cgcgcgcgcg cggcaggttc gccgtgaagg tgtcgtcatc aaagaaaaat
     6301 tcctgcactt gcggaaacaa ttttttggcg tgggccattt ctgccgcgac attttccgcc
     6361 gaacgaacgc gatacttatg tccgccgatg gtctgcggcc aaaggcagaa cgtgcattgc
     6421 gccggacacc cacgcccggt gtaaagcgat acgtaagggt ctttcagata gccgatggaa
     6481 tatttttcaa tctgcaaatc gcgcttgtaa acgtccgtca cccacggcaa cgcgtccatg
     6541 tcctggatca attcgcgctc gggattgtgg acgattttgc cgtccttgcg atacgaaagc
     6601 ccgttgattt cgctgagcgg ccggccttcg gcaacgtcct tgcaggtgaa atcaaattcc
     6661 ttgcgtccga cccaatcaat cgcaggcgat gctttcaacg tttcggtcgg caaaacggcc
     6721 gcgtgcgcgc caacaaaacc gatcgccgtg ccgggctttt gcgccttgat gg
//
LOCUS       DASZMM010000268         7560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_668049, whole genome shotgun sequence.
ACCESSION   DASZMM010000268 DASZMM010000000
VERSION     DASZMM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668049"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VG754_06475"
     CDS             <1..1138
                     /locus_tag="VG754_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18894.1"
                     /translation="VSQLGSERNCSLATNVGNYQEYDQGAVTFSNLTGTTWTDFKFGV
                     SRAGNGGGYKLALDYIKLVPQTPAQAPQAPTGLSASGGATDQIVLGWNASAWATSYTL
                     RSSTTSGGPYSIVATNITTLSYTNSGLSSNTVYYYVVSAMNSTGQSANSAETNATPGI
                     IIVDDASPVGVTITGSWTSSTSTPGYYGIDYLHDGDTGATGGKSVKFSPNIVVGGSNN
                     VYLRWTAAANRATNAPVDVNYAGGKQTFSINQQNNGGQWVLLGTYNFNAGTNGSVVVR
                     NDGASGYVIADAVEFVSVATDPPAPGVVAQRPQFTNVIMQTNGQFQLHFKGDTNSSYH
                     VWASTNLVNWQDLGPGVLTTNGAFQFIDAGSTNQPQRFYRVVNP"
     gene            1216..3315
                     /locus_tag="VG754_06480"
     CDS             1216..3315
                     /locus_tag="VG754_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWF18895.1"
                     /translation="MNDHALPAIAGKVLIWYGFTVVILFFGGFPALSHAQELPGAQSV
                     LTNIAEIRELMPHAAERHLPVHVRAQVTFGNLGRLCFLQDDTGAIFIRRKPDAVYLRP
                     GQLVEMTGVALPGDWAPIVREDSVKIIGESPLPPPKVLTFDEFNAGKADCQWVEVRGI
                     VRIARAIADHLHLEIAVGGGRMRAYDYNCPYKNVPSLVDSTIRIRGAMGGIFNHNGQF
                     AAPLLFASGTNSLTVERAAPSSPFEAPLRPADSLLRYVPGVDYDHRVRIAGIVTYQEP
                     AQMVFVRDGLQGLRIQTSEKILVKPGDRIEAVGFPVMGQYTPALEDAIFRLTASGTPP
                     APLETTIGDVLSGKMDANLVTLDARLIDFFLRANEYILVLQDSNIIFSAHLPYTESSP
                     VKLEKESRVRIAGICSIRNVHESVSFLTPQSFELLLRSPGDVTILERPSWWTLSRVLW
                     ILCAMAVVILIGVAWVVVLNRRVREQTQILHQRVQREAALEERNRIAREFHDTLEQEL
                     AGIALQLDTVAVHMKGSPGIAVRQLEMARNLSRHTLVEARRSVWDLRSHLLANNNLAT
                     ALAEVARQFESAQTKIEVQTSGAPRKLPARVENNLLRITQEALTNTLKHSRAKSVFVR
                     LDYEAQKTRLTIQDDGIGFNLKQVGGIEGGHFGLLGMRERAERSGASFLLQSAPGSGT
                     RIIVESMGTASLSQNNC"
     gene            complement(3478..5601)
                     /locus_tag="VG754_06485"
     CDS             complement(3478..5601)
                     /locus_tag="VG754_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF18896.1"
                     /translation="MELIRLDKIQKTYYRGELEIPVLKDVSLTIERGELIALVGVSGS
                     GKSTLMNILGCLDRPTSGKYWLDGEDISNVSGEQRAMVRNTKIGFVFQNFNLLPRTSA
                     LQNVLMPLNYTASHLTEKECRERAEKMLQLVGLGERMDHQPSQLSGGQQQRVAIARAL
                     INHPPVLFADEPTGNLDSRTAEDVLQIFQKLNAEEGITIILVTHDENVARHARRVIRI
                     KDGMIIEEGTPSAKPTGMGENSKLETRPAETGWDVVKTALRTLRLALHALSRNVMRSM
                     LTCLGIIIGIAAVIAMMEIGRGSSTSIEQTIASLGANVIQMDPNDTVVAGVSSGGGGR
                     VTLTPADADAVRAECSAVRWVAPSVDCRTQVIYGSLNWNPDRILGTTPDFLTIRKWDI
                     VDGDCFNDDDVRSAHPVCVIGQTVARQLFGKEAPVGKEIRVKNVGMKVVGVLSRKGAN
                     MQGRDQDDIIIAPWSTVKFRITGVRQSSSTSASAAASAVNTLNQVYPNQSVVLYPQQS
                     AVQAADMPQMTRFTDLDDVWISAASPPDIPVAMRQITALMRERHHIQPGAPNDFRIRD
                     LTELSQALASTSRVMTNLLLIVALISLVVGGVGIMNIMLVSVTERTREIGVRMAVGAK
                     AQDILRQFLVEAVVLCLAGGIAGIALGRGASITITVLLHWPTLASLPAIIAAVAVSVT
                     VGIVFGYYPAWKASRLDPIEALRYE"
     gene            complement(5617..6924)
                     /locus_tag="VG754_06490"
     CDS             complement(5617..6924)
                     /locus_tag="VG754_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF18897.1"
                     /translation="MQRFLKIPVLAVAIVILAGAGIWWWRIRSSPKVSFHTAVVKRGD
                     VSATISATGTIEPEEVVDVGAQVAGLITGFGTDITGKTIDYGSIVEKDAVLAKIDDSV
                     YAADLAVAKAQMEQSKAGELSAAANVDQMKARLVQAEAEWKRAQALTEFKVLARVDFD
                     TDQANYEVSKANVAVAEAAVAQAKAATVQAQAGLEKAQRNVDFCIIKSPVSGVIIDRR
                     VNIGQTVVSSLNAPSLFLIAKDLTKMQVWVAVNEADVGRIVPGAPVTFTCDAFPGRQF
                     EAKVGKVRLNATMTQNVVLYTVEVNAENPDGTLLPYLTANVSFLLHKETNTFLVANAA
                     LRWSPSSLAEISPAARSQGSFDPPPADAKPDKKSKEVSGIVWLKDGDYVRPLEVKVGT
                     SDGAFTAVSAQDLQEGLEVVTSDAAEKTQVATLNPFQPRIIRR"
BASE COUNT         1627 a         2155 c         2124 g         1654 t
ORIGIN      
        1 agtgtcgcag ttgggaagcg aacgaaattg ttcccttgcc accaacgtgg gcaattacca
       61 ggaatacgac cagggcgcgg tcacgttcag caatctcacc gggacgacgt ggacggattt
      121 caaattcggc gtcagccggg ctggaaatgg cggtggttac aaattggcgc tcgactacat
      181 aaaactcgtt ccccaaacac ccgcacaggc gccgcaagca ccgacgggac tcagcgcttc
      241 cggcggcgcg actgaccaaa ttgttcttgg ttggaacgcc tcagcctggg cgaccagtta
      301 tacgctgcgc agttccacaa ccagtggcgg accttacagc atcgtggcca caaatatcac
      361 gactttgagt tacacgaatt ccgggttgag cagcaacacg gtatattatt atgtggtttc
      421 ggcgatgaat tcaaccggcc aaagcgccaa ctccgccgaa acaaacgcca cgccgggaat
      481 tatcatcgtg gacgacgcca gtcccgttgg cgtcacgatc accggttcgt ggacttcgag
      541 cacttccacg ccaggttatt atggcattga ttatcttcat gatggcgaca ccggcgcgac
      601 cggcggaaaa agcgtaaagt tttcgcccaa tattgtcgtt ggaggttcca ataatgtcta
      661 tcttcgatgg accgccgccg ccaaccgcgc cacgaacgca ccggtggatg tgaattacgc
      721 gggcggcaaa caaaccttca gcatcaatca acaaaacaac ggcggccaat gggtgctttt
      781 gggcacatac aatttcaatg ccggcacgaa cggcagcgtg gtggtgcgca acgacggcgc
      841 gagcggatat gtcattgccg atgcggtgga gtttgtatcg gtcgcgaccg atcctcccgc
      901 gccgggagtg gttgcgcaaa ggccgcaatt tacgaacgtg atcatgcaga cgaacggcca
      961 attccagttg catttcaaag gcgacaccaa ttccagctac cacgtatggg catccaccaa
     1021 tttggtgaac tggcaggact tgggtccggg cgttttaacg acgaacggcg cattccagtt
     1081 tattgatgct ggttcaacaa atcaaccaca acggttctat cgcgttgtaa atccatgacc
     1141 ttatcttgaa tcggaagtgg cgaactggtc cgttgcccgt tgcggcttct gaggttatgg
     1201 ttcattttca tgcggatgaa tgaccatgcg cttccggcaa ttgccggaaa ggttttgatt
     1261 tggtatggtt ttactgttgt cattttgttt ttcggcggct ttccagccct gtctcatgcg
     1321 caagaattgc cgggtgcgca gtccgtcctc acaaacatcg cggaaatccg ggaattgatg
     1381 ccacatgcag cggagcgcca tcttccggtc cacgtccgcg cccaggtcac cttcggaaat
     1441 ctcggacgct tgtgtttcct gcaagacgat actggagcga tttttatccg gcgaaaaccc
     1501 gatgccgttt atttgcgtcc aggccagttg gtcgaaatga ccggcgtcgc gctgccgggc
     1561 gattgggccc cgatcgtccg cgaggacagc gtgaaaataa tcggtgaaag ccctttaccg
     1621 cctcccaagg ttctgacatt tgacgaattc aacgccggca aggctgactg ccaatgggtt
     1681 gaagtgcggg gaatcgtccg cattgcccgc gcgattgccg accatctgca tctggagata
     1741 gccgtgggcg gcggacggat gcgcgcatac gattacaatt gtccttacaa aaatgtccca
     1801 tcccttgttg attcgaccat ccggatacgc ggcgcaatgg gcggcatttt caatcacaac
     1861 ggccagtttg ccgcgccgct tctttttgcc agcgggacaa acagcctgac tgtcgaaagg
     1921 gctgctccaa gcagtccatt tgaggcgccg cttcggcccg cggacagtct gttgcgttat
     1981 gttcccggcg ttgattacga ccatcgcgtc agaattgccg gcatcgtcac ttatcaagag
     2041 ccggctcaga tggtttttgt tcgcgacgga ctgcaagggc ttcgcatcca aacatcggaa
     2101 aagattcttg ttaaacctgg cgacaggatt gaagccgtcg gctttccggt gatgggccaa
     2161 tacacacccg cgctggagga tgctattttc cggctcaccg cttcagggac gccgcccgcg
     2221 ccgcttgaaa ccaccatcgg ggacgttctc agcggaaaaa tggacgccaa tctggtcacg
     2281 ctcgacgcgc gactgattga tttttttctg cgcgccaatg aatacattct cgttttgcag
     2341 gacagtaaca tcatctttag cgcgcacttg ccttacaccg agtcttcacc cgtgaagctg
     2401 gaaaaagaga gccgcgtccg gatagccggc atctgctcga ttcgcaatgt ccatgaaagc
     2461 gtgtccttcc tcacgccgca gtcctttgaa ctgctgttgc gatcgccggg ggacgtcacc
     2521 atccttgaac ggccttcgtg gtggacgctt tctcgcgtgc tatggatact ttgcgccatg
     2581 gcggttgtca tcctcatcgg cgtggcgtgg gtggtggtgt tgaaccgccg cgtgagggaa
     2641 cagacgcaaa tcctccatca acgcgtccag cgtgaggcgg cgctggaaga acgcaatcgc
     2701 atcgcgcgcg agtttcatga cacgctcgaa caggaactgg ccggtatcgc gttgcaactg
     2761 gacactgtgg cagtgcatat gaaaggctcc cccggcattg cggtgaggca attggagatg
     2821 gcccgcaacc tcagccgcca tacgcttgtc gaggcgcgcc gctccgtgtg ggacctgcgg
     2881 tctcatcttc ttgccaacaa caatctggcg accgcgcttg cggaagttgc gaggcaattc
     2941 gagagcgcac agacgaaaat cgaggttcag acctccggcg cgccgcgcaa acttcccgcc
     3001 cgcgtcgaaa ataatctcct tcgcatcacc caggaagccc tcactaatac cctgaaacat
     3061 tcccgcgcaa aaagcgtttt tgtcaggctt gattatgaag cgcaaaagac gcggctcaca
     3121 attcaagatg atggcatcgg cttcaatctc aagcaagtcg gcggaattga aggcggccat
     3181 tttggtttat tgggaatgcg ggaaagagct gagcggtccg gcgcctcatt tttgttgcaa
     3241 agcgcgcctg gttcaggaac caggatcatc gtggaaagca tgggtaccgc atctttgagc
     3301 cagaacaatt gttgaattta tcggctccag agatcacaga aagtcacaga tggtcccact
     3361 tttttttggc gcgcaagcga agcttttgat ttacgtgatt tgtgaagtcc tgcatttttt
     3421 tgctctgacc gtgttgagca agcgtgcgcg aattcgacgt tcgccttcag ggctgcgcta
     3481 ttcgtaacgc aacgcttcaa tggggtcgag ccgcgaggct ttccacgccg gataataacc
     3541 aaaaacgatg ccaacggtca ccgaaacggc aacggccgcg atgattgcgg gcagggatgc
     3601 aagcgtcggc caatgcaaca agacggtgat ggtgatcgag gcgccgcggc ccagcgcaat
     3661 cccggcaatg ccgcccgcaa ggcaaagcac gacggcttcg acgaggaatt gtcgcaagat
     3721 gtcctgggcc tttgcgccca cagccatgcg aactccgatc tcacgcgtgc gttcagtgac
     3781 cgacacaagc atgatgttca tgatgccgac accgccgaca accagcgaga tgagcgcgac
     3841 gataagcagc aggttggtca tcacgcggct ggtcgaagcg agcgcttgcg aaagttcggt
     3901 caggtcgcgg atgcggaagt cgttgggcgc accgggctgg atatgatgac gttcccgcat
     3961 caacgcggtg atctggcgca tcgcaaccgg gatgtccggc ggcgaggcag cggaaatcca
     4021 gacgtcgtcc agatcggtaa agcgcgtcat ctgcggcatg tcagccgcct gcactgcgga
     4081 ttgctgcggg taaagcacca cggattggtt ggggtaaact tggttgagcg tgttgaccgc
     4141 ggaagcggcg gcgctggcgc tggtcgagga ggattgccgg acgccggtga tgcgaaattt
     4201 gacggtgctc cacggcgcga tgatgatgtc gtcctggtcg cggccttgca tgttcgcgcc
     4261 tttgcgggaa agaacaccaa cgactttcat gccgacattt ttaacgcgga tttccttgcc
     4321 gaccggcgct tccttgccga acaactggcg ggcgacggtt tgtccgatga cacacaccgg
     4381 atgagcgctg cggacatcat catcattgaa gcaatcgcca tcaacgatgt cccacttgcg
     4441 gatggtgaga aaatccgggg tcgtgccgag aatgcggtca gggttccaat tgagacttcc
     4501 gtaaatgacc tgcgtcctgc aatccacgct cggagcgacc caccgcaccg cgctgcattc
     4561 ggcgcgaacc gcgtctgcat ctgccggggt gagggtgacg cgtccgccgc ctccggaact
     4621 cacgccggcg acgacagtat cattggggtc catttggatc acgttcgcgc cgaggctggc
     4681 gatggtttgc tcaatggatg tcgaagaacc gcgtccaatc tccatcatgg caatgaccgc
     4741 ggcgattccg atgatgatgc cgagacaggt cagcatggat cgcatgacgt tccggctcaa
     4801 tgcatgcaac gcgagtcgca acgtgcgaag agcagttttg accacgtccc atccggtctc
     4861 cgctggccgc gtttcgagtt tggaattttc gcccattccg gttggttttg ccgacggcgt
     4921 tccttcctcg atgatcattc catccttgat gcggatgacg cgccgggcgt gtcgcgccac
     4981 attttcgtcg tgcgtcacaa gtatgatcgt gatgccttcc tcggcattga gcttctgaaa
     5041 aatctgcaac acatcctccg cagtgcgcga atcaagattg ccggtcggct cgtcggcaaa
     5101 cagaacgggg ggatgattga tgagcgcgcg cgcaatggcg acacgctgct gttgcccgcc
     5161 ggaaagctgg gacggctgat ggtccatccg ttcgcccaat ccaacgagct gcagcatttt
     5221 ttcagcgcgc tcgcggcatt ccttttccgt caaatggctc gcggtataat tcagcggcat
     5281 caggacgttt tgcagtgcgc tggtccgggg cagcaggttg aaattttgaa agacaaaacc
     5341 gatcttggtg ttcctcacca tggcgcgttg ttcgccagaa acattggaga tgtcctcgcc
     5401 gtccagccaa tattttcccg aagtcggccg gtcaaggcag cccaggatgt tcatcaacgt
     5461 gctttttccc gaaccgctga caccgaccaa agcaatcaat tcgcctcgtt caatcgtgag
     5521 cgacacatcc ttcaggacgg gaatctccaa ttcaccccga tagtaagtct tctggatttt
     5581 gtcgagtcgg atgagttcca tggccggcgc tctttttcaa cgtcggatga tccgcggctg
     5641 gaacggattc aacgtggcga cctgcgtctt ttccgcggca tcgctggtga caacttccag
     5701 tccttcctgc aaatcctggg cagaaactgc cgtaaatgcg ccatccgaag tgccgacctt
     5761 cacctccagc ggacgcacat aatcgccatc cttcaaccag acgatgccgg aaacctcttt
     5821 ggattttttg tctggtttcg cgtcggcggg cggcgggtcg aatgagccct gcgaacgggc
     5881 ggcaggcgaa atctccgcca acgatgacgg cgaccagcgt agggcggcat tggcaaccag
     5941 aaacgtgttg gtttctttat ggaggagaaa actgacattg gcagtaagat acggcagcaa
     6001 cgttccgtcc ggattttccg catttacctc gacagtgtat aacacgacgt tctgcgtcat
     6061 cgtggcattg aggcggacct tgccgacctt ggcttcaaat tgtctgccgg gaaaggcatc
     6121 gcaggtaaat gtcaccggcg cgccgggaac aattctgccc acgtccgcct cgttgaccgc
     6181 gacccagacc tgcatcttgg tcaggtcctt cgcgatgagg aaaagactcg gcgcgttcag
     6241 gctggacacc accgtttggc cgatgttcac acgccgatcg atgatgactc cgctaaccgg
     6301 cgatttgatg atgcagaaat ccacattgcg ttgcgccttt tccaaaccag cctgcgcctg
     6361 cacagtggcg gccttcgctt gggcgacagc cgcttccgcg actgcgacat tcgccttgga
     6421 aacttcatag ttggcctggt ccgtgtcgaa gtccacgcgg gcaagcactt tgaattcggt
     6481 caatgcctgc gcccgtttcc attccgcctc ggcctgcaca agccgggcct tcatttgatc
     6541 gacgttcgcg gcagcgctca actcgcctgc cttgctctgc tccatctgcg ccttggccac
     6601 cgcgagatca gcggcataga ccgaatcatc aatcttcgcc agcacggcat ctttctccac
     6661 gattgaaccg taatcgatcg tcttgcccgt tatgtctgtg ccgaaacccg tgatcagccc
     6721 cgccacctgc gcgcccacgt ccacgacctc ctcgggttca atcgtgccgg tcgcgctaat
     6781 ggtcgccgac acgtccccac gcttcaccac ggccgtatgg aaggaaacct tgggactgct
     6841 tcggatgcgc caccaccaga tgccagcccc cgcaagaatg acgattgcaa ccgcaagaac
     6901 agggattttc agaaatcgtt gcatcaagcc gcagtcaaac gttgttgaag ccaaacgcgg
     6961 cgagatgaca ttccattcga agcgattgca ttattttccg gcatgatcgt atcgctgctt
     7021 aatatcactt gatcgcattc tgtggaatgt tttgtgcgcc cgctgccgtg catgttcctg
     7081 cgcgtggcag cggaaggaaa actaaacctg ttcccttgcg gcaaatcaaa cgttttgcgc
     7141 aatatgacga agaattaatt tcggaacggg ctgccagcct tgttccatga gggacgaaat
     7201 tgggtgaatg agtttcgatg gggcgcatct cagtttcttg cacaacggag tggttgaaca
     7261 tttcctcccc cacaccccag ccctctccct tggggagagg gagcaacgca ttccgtcgtg
     7321 gttgcaaacc aagagcgttc gatttatcga gcagcggcca aatggtcccc ctctccttgg
     7381 ggagagggct ggggtgaggg ggacagcgat gtgcgccgac ccatgaacac aattcgcaag
     7441 agcccagaaa cgtgcaaaat agtgagatgc gcccgttcat gcgccgcaaa ccaaccgttt
     7501 cccgcacgga aggtcaagcg gtattcggaa tggccacaaa gaggcacaaa aggagcaaaa
//
LOCUS       DASZMM010000269         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_97121, whole genome shotgun sequence.
ACCESSION   DASZMM010000269 DASZMM010000000
VERSION     DASZMM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97121"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..528
                     /locus_tag="VG754_06495"
     CDS             67..528
                     /locus_tag="VG754_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWF18898.1"
                     /translation="MKSHRHHHQHSHSRSNLPELTERLRRKARRITGPRQAILEILRR
                     HPHPMAIKEIFAELPKGDCDLATVYRSMHLLEGMKMVKRFDLGDSVARFELLAEGDDG
                     HHHHLVCVRCSAVIEINECFTRELEEKMAARSGFKAITHKLEFFGICPECQ"
     gene            533..910
                     /locus_tag="VG754_06500"
     CDS             533..910
                     /locus_tag="VG754_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18899.1"
                     /translation="MTNLLKKSHWRAGSKIKNLIKKSGGFFSYPFTDMQVLLDFPAFI
                     PVFLGFFGLVVGFAEGMFVTTYGFTEYFQRFYHNWHSFLQGYTSVSHANPADVFLVSR
                     QGCWHQSRFFQLLRRMWLFGVQK"
     gene            complement(985..1884)
                     /locus_tag="VG754_06505"
     CDS             complement(985..1884)
                     /locus_tag="VG754_06505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWF18900.1"
                     /translation="MKREPLWQVSVTISLEAEEAVVELLARVLERAAAVYTDEETKIT
                     IASVYCSKRSEWNSRKQAALADGLKTIAASGLNVGAGKISAQRVAREDWSESWKRHFK
                     PIEIGWRLLIKPSWIKRRPRKNQAVVVLDPGLSFGTGNHPTTAFCLRQLAMGRKAGQR
                     QSFWDIGTGSGILAISAVKLGYAPVRAMDFDPEAVRVAKENARKNKIWKKIQITREDI
                     TKLPATSGSKYDFICANLISNLLIAECRRIVNRLRPGGTLVLAGILREEFASVRIAYE
                     VRGLHFAESQIDGEWESGAFRLV"
     gene            complement(2038..2658)
                     /locus_tag="VG754_06510"
     CDS             complement(2038..2658)
                     /locus_tag="VG754_06510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWF18901.1"
                     /translation="MIAATSLFLLRHAEVEERYHRIFGGRIDMELSPRGHEQAAILAR
                     HLRHKSFDAIYASPMKRVQQTLAPLLEGRNAAPVVMAGLREVDFGDWTGLRWEQVKEK
                     YNISAFDWLSQLEHAAIPNAESSRTFRARVEPCVKQILREHPGQTVAIACHGGTIRMI
                     LSILLEMPLPQMASFEIEYASLSQVHHWPHKAEVQLLNFTPWRDAA"
     gene            complement(2676..3428)
                     /gene="rph"
                     /locus_tag="VG754_06515"
     CDS             complement(2676..3428)
                     /gene="rph"
                     /locus_tag="VG754_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWF18902.1"
                     /translation="MQEPSSTTAASPRVDGRLPNQLRPLRFQNHIAPYATGSTLVEWG
                     ATRVICGVTVEDAVPRWMKEQNVLGGWITAEYSMLPYSTLQRKQRDISKGKIDGRSQE
                     IQRLIGRAMRAAIDLEKLGQRTIWVDCDVLQADGGTRTAAITGAYVALSLAVKKLTGE
                     GKLKENPMLHPVAAVSVGIVSGQPLLDLCYVEDAAAAVDLNLVMNAAGEFIELQGTGE
                     EATFSESQLAALLGLGKAGIRELLAAQQAALA"
     gene            3569..>4186
                     /locus_tag="VG754_06520"
     CDS             3569..>4186
                     /locus_tag="VG754_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HWF18903.1"
                     /translation="MKLPFRTFATCLFPALLLPLCSRGETNPAIDRAMAAVQAAAPRA
                     QSDPDRPIFHVTSPAQWINDPNGPIYYKGFYHLFYQLHPFSDESGPKYWGHVRSRDLA
                     KWETLPIALWPSTELGEAEVWSGCCTINGEGKPMIFYTSIAPGKSAQTHAEQWGAIGD
                     DDLITWRKSPANPLLSETLHGGKKIYEWRDPFIFQDHGRNFLVTGG"
BASE COUNT          880 a         1246 c         1108 g          952 t
ORIGIN      
        1 ctttttcttg ctgacattgg cgctgggacg gttccgcgcc gagaggccgt tgattgcacc
       61 tgttaaatga aatctcaccg gcatcatcat cagcattctc actcccggtc gaatttaccg
      121 gaactcaccg aacggctccg ccgcaaggcg cgccgaatca ccgggccgcg ccaggcaatc
      181 ttagaaattt tgcgccgcca tccgcatcca atggccatca aggaaatttt cgccgagttg
      241 cccaagggcg attgcgatct ggcgacagtg tatcgctcca tgcacctgtt ggagggcatg
      301 aagatggtga agcgttttga tctaggcgac agcgtggcgc gtttcgagct tttggccgag
      361 ggagacgacg gacatcacca tcatctcgtc tgcgtccgtt gttcagcggt gattgaaatc
      421 aacgaatgtt tcacccgcga actggaggag aaaatggccg cgcgcagcgg cttcaaggcg
      481 atcactcaca aattggaatt tttcggaatt tgtccggagt gtcagtgact ccttgaccaa
      541 tctgctcaaa aagagccatt ggcgcgcggg ctcaaaaata aaaaacctga ttaaaaaatc
      601 aggtggcttc ttcagttacc cattcaccga tatgcaggta ctccttgatt ttcctgcgtt
      661 catacctgtg tttctgggct tttttgggct ggtcgtcggc tttgcggaag ggatgtttgt
      721 tactacgtat ggcttcactg aatacttcca acgtttttat cataactggc attcctttct
      781 tcaaggttac actagcgtgt ctcatgccaa cccggctgat gtgttcctcg tcagccggca
      841 aggctgttgg catcaatcaa ggttctttca acttcttcga cgaatgtggc tcttcggtgt
      901 tcaaaagtaa tttgttaaat cgaggtgtca agttggcgcg tgcaggtaaa atgagaatcc
      961 tgcgagaaga cttaaagagt catttcacac caaccgaaac gctcccgatt cccattcgcc
     1021 gtcgatctgg ctttctgcaa aatgcaagcc tcttacttcg taagcgattc gcacagatgc
     1081 gaattcctcc ctcaagatgc ctgcaagcac caacgtgcct cccggccgca accggttcac
     1141 aatgcgtctg cattctgcaa tcaagaggtt ggaaatcaaa ttggcgcaga taaaatcgta
     1201 tttcgacccg ctggttgccg gaagtttggt gatgtcttcg cgcgtgatct gaattttttt
     1261 ccagattttg tttttgcgcg cattctcctt ggcgacgcgc acggcttccg gatcaaaatc
     1321 catggcccgc accggcgcgt aaccgagttt caccgctgaa atcgccagga tgcccgaacc
     1381 ggtgccaatg tcccaaaatg actggcgttg tcccgctttg cggcccatcg cgagttgccg
     1441 cagacaaaac gctgtcgtcg gatgattgcc agtgccgaag ctgagccccg gatcaagcac
     1501 cactacggcc tgatttttgc gcggacgccg cttgatccaa cttggcttga tgagcagccg
     1561 ccaaccgatt tcaatcggct tgaaatgccg cttccacgat tccgaccaat cctcccgcgc
     1621 gacgcgttgc gccgaaattt ttcccgcgcc aacgttcaat ccgctcgcag caatcgtttt
     1681 caacccatcc gccagcgcgg cttgtttacg cgaattccat tccgagcgtt tcgagcaata
     1741 gaccgaagcg atggtgattt tcgtttcttc gtcggtataa accgctgctg cccgttccaa
     1801 gaccctcgcc agcaattcca ccaccgcctc ctccgcttcc agcgaaattg tcaccgaaac
     1861 ctgccacaat ggttcgcgct tcattcaaaa atctcgttcc gttctgagac aattcaggcg
     1921 actggaaagt cgcctgcacc cgcacactgg aaagtgtgcg ctacgtggcg caggctttcc
     1981 agcctgcggg ttccggggac tttccagtcc ccggagacgt ttccaacgtg ccgcgcttca
     2041 tgccgcatcg cgccacgggg tgaaattgag caactgcacc tcggccttgt gcggccaatg
     2101 atgcacctgc gaaaggctcg cgtattcaat ctcgaatgac gccatttgcg gcagcggcat
     2161 ttcgagcagg atggaaagaa tcatgcgaat cgtcccgccg tggcaggcga tggcgacagt
     2221 ttggccggga tgttcgcgca agatttgttt cacgcacggc tcgacgcgcg cgcgaaatgt
     2281 cctggaagat tctgcattcg gaatcgccgc gtgttccaat tggctcaacc agtcgaacgc
     2341 gctgatgtta tatttctcct ttacctgttc ccagcgcaat cccgtccaat cgccaaaatc
     2401 cacctcgcgc aaaccagcca taaccaccgg cgcggcattg cgcccttcga gcaacggcgc
     2461 gagcgtctgc tgcacgcgtt tcatcgggct ggcgtaaatg gcgtcgaaag atttgtgcct
     2521 gagatgccgc gcgagaatgg ccgcctgttc gtgtccgcgc ggggaaagct ccatgtcaat
     2581 gcgtccgccg aagatgcgat ggtaacgctc ctcgacttcc gcatggcgca aaagaaaaag
     2641 actcgtcgca gcaatcatcc cgaaaattcc ttggctcaag ccagcgcggc ctgttgcgct
     2701 gcgagcagtt cgcggatgcc cgccttgccc agccccagca acgcggccag ttggctttcg
     2761 ctgaacgtcg cctcctcgcc ggtgccttgc aactcaatga attccccggc ggcattcatc
     2821 accaggttca aatccaccgc cgccgccgcg tcctccacgt aacacagatc gagcaacggc
     2881 tgcccgctca cgattcccac gctcaccgcc gcgaccgggt gcagcatcgg attttccttg
     2941 agcttgcctt cgcccgtcaa ttttttcacc gcgagcgaca gcgccacgta agcgccggtg
     3001 atggccgcgg tgcgcgtgcc gccgtccgcc tgcaacacgt cgcaatccac ccagatggtg
     3061 cgctggccaa gtttttccag atcaatcgcg gcgcgcatgg cgcggccgat gagccgctgg
     3121 atttcctgcg aacgcccgtc aatttttccc ttcgagatgt cgcgctgttt gcgttgcaga
     3181 gtggagtagg ggagcatgga atattccgcg gtgatccagc cgccgagcac attttgctcc
     3241 ttcatccagc gcggcaccgc atcttcgacc gtcacgccgc aaatcacgcg cgtcgcgccc
     3301 cattcaacca gcgtcgagcc cgtcgcgtag ggcgcgatgt ggttctggaa acgcagcggc
     3361 cgaagctgat tgggcagacg cccatcaacc cgcggcgacg cggcagtggt tgacgatggt
     3421 tcttgcatag ggcggcgcga tgttaaagga gacagcgacg caagggcaac gatgaaagtt
     3481 aagcgaaaga cggcgaattt tccacaacca cgcgagcgcc attgcaaatt attttcacgt
     3541 tgggcaaaat tcttttcata cggaccaaat gaaactgcca tttcgaactt tcgcgacctg
     3601 cctgttcccc gcattgcttc tgccgctttg ctcgcgggga gagacgaacc cggcgattga
     3661 tcgcgcgatg gccgcggtcc aagccgctgc gccgcgcgcg caaagcgatc ctgaccgccc
     3721 gatttttcac gtcacatctc cggcccagtg gatcaacgac ccgaacggcc cgatttatta
     3781 caaagggttt tatcatctct tttatcaact ccatccgttc agcgatgaaa gcgggccgaa
     3841 atattgggga catgttcgca gccgtgattt ggcgaaatgg gagacgttgc caatcgcgct
     3901 ttggccctcc accgaacttg gcgaagcgga agtctggtct ggttgctgca cgatcaacgg
     3961 cgaggggaag ccgatgattt tttacaccag cattgcgcct ggaaaatccg cgcagaccca
     4021 tgccgagcaa tggggcgcga tcggcgatga tgatttgatc acctggcgca agtcgccagc
     4081 caatccgttg ctgagcgaaa cgttgcatgg cgggaaaaaa atctacgaat ggcgcgaccc
     4141 gtttattttt caggaccatg ggcgcaactt tctggtcacg ggcggc
//
LOCUS       DASZMM010000270         7374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_669306, whole genome shotgun sequence.
ACCESSION   DASZMM010000270 DASZMM010000000
VERSION     DASZMM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669306"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VG754_06525"
     CDS             complement(<1..2226)
                     /locus_tag="VG754_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWF18904.1"
                     /translation="MEPKTMPIEPKDGARPADATAAAPDEPTFVRKLDHPYFELRTPD
                     AQTNSYLEGNAQKMLTADQEKLHVFLAEARLVMDQSTTPRKRSVHYKIDAEKYKNIRP
                     MDALPPSEFAAFEAAAKDFYDKAAPDRKDVHPYETFLRLNFRLPDPDKEPDAYLVYGE
                     DFERRLLILWGCEKEKDSALPLAKHAVVRPRRPDDTVLDRLRRKIIGWEQMQKDTLLL
                     LAKTREPLFRFLGQPVMDDKGTLKGVKIGEIALSTKELRPLRYLLPPEMKLFYKACQE
                     FYARAVTAKSPYEKELRTNFQLPDPEKMPEAFRVHGPINHPRLVIVFDGSARREQTIW
                     LTPVPEQNIPPMPPVTPEQELLGHKPVPPLTLTDKLQRRTIPFAKIFSVAGAIVATPL
                     VIAFLVWLALDKSPPRPLKEIVPGSDATSILVRFNEPIDPVSVPKAATLIPKSFVLVE
                     SNGGPVTITSATLNTTGDYKNREIALASTTPFTDGNGYRLSVNGLADRALLHNQMRNA
                     VQVGFIYADNKPPVLGMPSGDGESLSHLVLPFNKNMDATSIAETANYSIDGANYKVSA
                     ATLDEDKKSVILTVSPDMPLKSEHTLIVKNVKDASHLGNNLKPDPTSVDFRFVDTMPP
                     KLRKVTAEEQNKVVLEFNEKLSPATVTNIANYSLASRKDGGKLDVYFAQLVDLKKIAI
                     FTAPLFKDIDYDLGVTKIGDMADPINIMSTEERRSFTLPPDRENRFAPRIESLSHQPD
                     RT"
     gene            complement(2279..3772)
                     /locus_tag="VG754_06530"
     CDS             complement(2279..3772)
                     /locus_tag="VG754_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18905.1"
                     /translation="MVKWLPILCLAIWPGVAGASDIGEIKTSLTNLIRQVEALQIQTA
                     ALRGSLDVTTNSFRNQLASVQNGLVTLKSAVEAKPAASDTSSARQMQFTASLRDLSNS
                     ILQLNGMVAELKSGAGKPDYKSEVDRQLAGLNARIAGLTNALVNLKPAGMDAETKENI
                     AFLKNNLARAGAQGAQPVWMNYTLLGLLAVSLGLSGWLLVSFRSQLESSEKRTRELLV
                     RFGSQMEGRVKSMEGSSVTVQNSAREITDFLGRVELAVHGQLETVAQQLNSAQVTELT
                     GGAVRAAEEMALLRTETAELKKFMGDLRQSTERRVANLEEQTGRTLEQIKENMNTPNE
                     DILRVLPAPFRDDGKLVEARERILTRFIAGDLTSHALLLGLAQLQLSIATRDGDTSLM
                     VEALDHLGRLAYRFWKGEPDASVESTFEFAQTWGSEINKLLTENNIPLNIRIIMPRAG
                     FDMGTMLSEHSATGTTTRVREPLSWAVVGKGPEQQSKVLVTGKVITE"
     gene            complement(3816..6710)
                     /locus_tag="VG754_06535"
     CDS             complement(3816..6710)
                     /locus_tag="VG754_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor SrfB"
                     /protein_id="HWF18906.1"
                     /translation="MPIELKVFPNTGHQYFTLELEKLCPNRDIGALNTLVMKHINDPW
                     QVWDPYPWVRYQYDESTDLGSTSGTTTSVPVIKIAVETNMSIPRNGLLWEIDSKLGQK
                     GNEDFLFPYASDDAAQITENLGDERSVPLFSLPNKEGKELPVDLHIVFAKPAGNDPID
                     VDLVVDLGNTRTAALLLETRGAPDKDQWPFSRRVKVLRVTPPGVQYDKVDKDCEIIPS
                     WLLMHRTTFAALEPNLSLSQLFDMYESYEDPSGKTYYRRKRYLPRSFIEISPAMVGGG
                     KSNKGVAKRLSDLALMSGRYYFSSPKRYVWDDAQTGSQGGVFWMQVKNPSDPDKRGSM
                     LTELGGMIRVYMDPESHDWDIENPPGDEDFLRRYPERPPSYPRRDAVCWFALSLIEAA
                     YRQINSRKYLDSTGYAELPRRLRHIMVTYPAAWNHEVKESFLAQWRRAINLFTITHFD
                     TDRHAPVTQGGARPVLVDANVNEAICSQLPIMYSDISNLMNKPDEWFQLYGDADQKVV
                     VMNLDIGGGTTDLTVIRYQSAPHGSMQPHLLFQDGRSTAGDELVKKIIEKLLLPAWIK
                     MGDQGQYKNFPEAKAWVLNLLKDPSNHEIAQVEPLAHARLARITRLLFIPLVNELLSS
                     MVGMEGNPEGRLEPLDLEQFGAMYETPLKELNDMTAAIIRAKCRNGNTWKGQVFPLKG
                     VRLSIPAADVDACIEDVFGEFCKSLGRVAAHFRCHLVIVTGKPSELPKLRQLITEAFP
                     ILPQRIIHARNFPAGRWYPFGSFGVGDTESGRIMDAKSCTVVGGALYLDSLKNPHLTR
                     FLTIEDTAGEMKGRYYWGVVPLQGNAREFYSSPNLLFSPNEYAKAIQHENRLTLEKDF
                     ELTINDCRIGRQVIRVENIRAEPVYELCFAPERGRNIMIQTPVRAKVRLRWVLEKGRG
                     ESLELVSVTPLEELDKMQDVRVRLRLNTLYESSFWLDDPRFEVDERAAQA"
     gene            complement(6861..>7374)
                     /locus_tag="VG754_06540"
     CDS             complement(6861..>7374)
                     /locus_tag="VG754_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18907.1"
                     /translation="EAWRKNGRRDKPDWSLLGLDSREKLRSWLEALVASLEGHLLPMA
                     VWLKDLVEYNLDRPGTDLRRILAIRMGNVLIYGKSGPPSYDSGIGDSASFDDISVDPK
                     GRTLKGRGCPAYKVFLQPFLEAQLKKLLNEGIGLIQRQAQPGDEQLRKLCMDNDCLPA
                     CAQSPDESKK"
BASE COUNT         1511 a         1958 c         2214 g         1691 t
ORIGIN      
        1 ggtgcggtcg ggctgatggc tgagcgattc gatgcgaggc gcaaaacgat tttcacggtc
       61 gggaggaagc gtgaaactgc ggcgttcctc ggtggacatg atgttgatcg gatcggccat
      121 gtcgccgatt tttgtcacgc caagatcgta atcgatgtcc ttgaaaagcg gcgcggtgaa
      181 gatggcgatt tttttcagat cgacgagttg cgcaaaatac acatcgagtt tcccgccgtc
      241 tttgcggctc gccagcgaat aatttgcgat gttggtgacg gtcgcgggac tgagtttctc
      301 gttgaattcg agcacgacct tgttttgttc ctcagcggtc accttacgca acttgggcgg
      361 catcgtgtcc acaaaacgaa aatccacgga agtcgggtcg ggtttgagat tgttgccgag
      421 atgcgaggcg tccttcacat tttttacgat caacgtgtgc tcgctcttga gcggcatgtc
      481 gggtgaaacg gtgagaatga ccgatttttt gtcttcatcg agcgttgcgg cgctgacttt
      541 gtaattcgcg ccgtcaatgg agtaattggc ggtttcggcg attgaggtcg cgtccatgtt
      601 cttgttgaac ggcagtacca gatggctgag actttcacca tcgcccgaag gcatcccaag
      661 gacgggcggt ttgttgtccg cataaatgaa cccgacctgc accgcgttgc gcatctggtt
      721 gtgcaacagc gcgcggtcag caaggccgtt gaccgagaga cgataaccgt tgccgtcagt
      781 gaatggggtg gtggatgcga gcgcgatttc gcggttttta taatcgcccg tcgtgttcag
      841 cgtggcggaa gtgatggtga cgggtccgcc attggactcg acaaggacga acgatttcgg
      901 aatcagcgta gcggcttttg gcaccgacac gggatcaatc ggctcgttga agcggacgag
      961 aatggaagtg gcatcgctgc cgggcacaat ttccttgagc ggacgcggcg gcgatttgtc
     1021 cagcgcgagc cagacgagaa atgcgatcac caacggcgtg gcgacgattg cgccagcgac
     1081 cgagaaaatt ttggcgaacg gaatcgtgcg acgttgcaat ttatcggtca acgtcagcgg
     1141 cggaacgggt ttgtgaccga gcaattcctg ttcgggcgtg accggcggca tcggcggaat
     1201 gttctgttcc ggaacgggcg tgagccagat tgtttgctcg cgcctggccg agccgtcgaa
     1261 aacaatcaca agccgcggat gattgatggg gccatgaacg cggaacgctt cgggcatctt
     1321 ttcggggtcg ggcaattgaa aattggtccg caattctttt tcgtaaggcg acttggccgt
     1381 gacggcgcgc gcgtaaaatt cctggcacgc cttgtaaaag agcttcatct ccggcggcag
     1441 cagataacga agcggacgaa gttccttggt ggatagcgca atttcgccga tcttcacgcc
     1501 tttgagtgtg cccttgtcat ccatgaccgg ctgaccgaga aaacggaaca gcggctcgcg
     1561 tgtcttggcg agcagcagca gcgtgtcctt ctgcatctgc tcccagccga tgattttgcg
     1621 gcgcaatctg tccagaaccg tgtcgtccgg tctgcgcggg cgcacaacgg cgtgtttggc
     1681 gagcgggagg gcggaatctt tttctttttc gcaaccccaa agaatcagca gccgccgttc
     1741 gaaatcttcg ccgtaaacca gataagcatc cggctccttg tcgggatcgg gcaggcggaa
     1801 attcaggcga agaaatgttt cgtaaggatg aacgtctttg cggtccggcg cggccttgtc
     1861 gtaaaaatcc tttgcggcag cttcgaacgc tgcaaattca ctcggcggca atgcgtccat
     1921 gggccggata tttttatatt tttccgcatc aatcttgtaa tgaacgctgc gcttgcgggg
     1981 cgtggtgctc tggtccatga caagccgtgc ctcggcaaga aagacgtgca gtttttcctg
     2041 gtcagcggtg agcatctttt gggcgttgcc ttcgagatag gaattggtct gtgcgtccgg
     2101 cgtgcgcaac tcgaagtagg gatggtccaa cttgcggaca aatgttggct cgtcgggagc
     2161 cgccgctgtg gcgtccgccg gtctggcccc gtccttgggc tcaataggca tcgttttcgg
     2221 ttccatgtgt gttcaggtgt gaaattattt cggcaaaaca aatcggcaga ttagccagct
     2281 attcggtgat gacctttcct gtgaccagga cttttgattg ttgttcagga cccttcccga
     2341 ccactgccca ggacaacggc tccctgaccc gcgtcgttgt gccggtggcg gagtgttcgg
     2401 acaacatggt gcccatgtca aaaccggcgc gcggcatgat aatcctgatg ttaaggggga
     2461 tgttgttttc ggtcaacagc ttgttaattt ccgagcccca agtttgggca aattcgaaag
     2521 tggattcgac tgacgcgtca ggttcacctt tccaaaatcg gtacgcaagg cggccaaggt
     2581 gatcgagcgc ctcgaccatc aggctggtgt cgccgtcgcg cgtggcgatg gacaattgca
     2641 attgggccag gccgagcagc agcgcgtggg acgtcaggtc tccggcgatg aagcgcgtca
     2701 aaatgcgttc gcgcgcctcg acgagctttc cgtcgtcgcg gaaaggcgca ggcaaaacac
     2761 gcaaaatatc ctcattcggc gtattcatat tttctttgat ttgttcgagc gttcgcccgg
     2821 tttgttcctc aagattcgcg acccggcgct cagtggactg acgcaggtcg cccatgaatt
     2881 ttttcaactc cgccgtctca gtgcgcagca gcgccatttc ctccgccgcc cgcaccgcgc
     2941 cgcccgtcaa ttcggtgact tgcgccgaat tcaactgctg cgcgaccgtt tccaattgcc
     3001 catgcaccgc caattccacg cgcccgagga aatcagtgat ttcccgcgcg gaattctgca
     3061 ccgtgacgct gctgccctcc atgctcttga cgcgcccttc catctgcgag ccgaaacgca
     3121 ccagcaactc gcgcgtcctt ttctcggacg actcaagctg gctgcggaaa ctcaccagca
     3181 accatcccga aagccccaat gacaccgcca acagacccag cagcgtgtaa ttcatccaaa
     3241 ccggttgcgc gccctgtgcg cccgcgcgcg cgaggttatt cttcaggaac gcgatgtttt
     3301 ccttcgtttc cgcgtccatg ccggcaggtt tcagattgac caaggcattt gtcaaacctg
     3361 caatgcgcgc atttaagccg gcaagctggc ggtccacttc gcttttgtaa tcgggcttgc
     3421 ccgcgcccga cttcaattcc gcaaccattc cgttgagctg gagaatgctg ttggacaagt
     3481 cgcgcaagga tgcagtgaac tgcatttgtc gcgcggatga tgtgtccgac gcagcaggtt
     3541 tggcttcgac ggcgcttttt aaagtgacca acccgttttg caccgacgca agctggttac
     3601 ggaatgaatt ggtggtgaca tcgagcgagc cgcgcaatgc ggccgtttgg atctggagag
     3661 cttcgacctg ccggatgaga ttggtgaggc tggtcttgat ttccccgatg tccgaagcgc
     3721 ccgccacacc cggccaaatg gcgaggcaaa gaatcggcaa ccatttcacc atgcgtgcaa
     3781 ccctcttttt aaggaacgtt gccatggcta tatcttcatg cttgagccgc gcgctcgtcc
     3841 acttcgaagc gcgggtcgtc gagccagaag ctgctttcgt aaagtgtgtt caagcgcagg
     3901 cgcacgcgga catcctgcat cttgtccaat tcttccagcg gagtcacaga gacgagttcg
     3961 agtgattctc ccctgccctt ttccaggacc cagcgcaggc ggaccttcgc gcggaccggc
     4021 gtctggatca tgatgttgcg gccgcgttcg ggcgcgaaac aaagttcgta aacgggttcg
     4081 gcgcggatgt tctcaacgcg gatgacctgc cgtccgatgc ggcaatcgtt gatggtgagt
     4141 tcaaaatctt tttcgagcgt cagccggttt tcatgttgaa tcgccttcgc gtattcgttc
     4201 gggctgaaga gaagattcgg cgacgaataa aattcgcgcg cgttgccctg gagcggcacg
     4261 acgccccaat aataacgtcc cttcatttcg ccggcggtgt cttcaatcgt caaaaagcgc
     4321 gtgagatgcg gatttttgag gctgtcgaga tagagcgcgc cgccaacgac ggtgcaggat
     4381 ttggcgtcca tgatgcggcc gctttcggtg tcgccaacgc cgaatgaacc gaatggatac
     4441 cagcgtccgg cggggaaatt gcgcgcatgg atgatgcgtt gcggaaggat gggaaaagct
     4501 tccgtgatca actggcggag ctttggtagt tcggaaggtt tgccggtgac tatgacaaga
     4561 tggcagcgga aatgggcggc aacacggcca agtgacttgc agaattcacc aaaaacatcc
     4621 tcgatgcagg cgtcaacgtc cgcggcagga atgctgagcc ggacgccttt gagcgggaaa
     4681 acctggcctt tccaagtgtt gccgttccgg catttggcgc ggatgatggc cgcggtcatg
     4741 tcgttgagtt ccttgagcgg cgtctcgtac atcgcgccga attgctccaa gtcaagcggc
     4801 tcaaggcgac cttcaggatt gccttccatc ccaaccatgc tggagagaag ttcgttgacg
     4861 agcggtatga aaagcagccg ggtgatgcgc gcgagccgtg cgtgcgcaag cggttcgact
     4921 tgggcaatct cgtgattcga cgggtctttg aggaggttca agacccaggc ttttgcttcc
     4981 ggaaaatttt tatactggcc ctggtcgccc atcttgatcc acgcagggag caaaagtttt
     5041 tcgatgattt ttttgacgag ttcatcgccg gcagtggaac ggccatcttg aaataaaaga
     5101 tgcggctgca tcgaaccatg cggcgccgat tgataacgga tgaccgtgag gtcggtcgtg
     5161 ccgccgccga tgtcgaggtt catcaccacg actttctggt cggcatcacc gtaaagttgg
     5221 aaccattcgt cgggtttgtt catcaggttg ctgatgtccg aatacatgat tggcaactgg
     5281 gaacaaatgg cttcgttgac attggcatca accagcacgg gacgcgcgcc gccttgcgtg
     5341 acgggagcgt ggcggtcggt atcaaaatgg gtgatggtga aaagattgat ggcgcggcgc
     5401 cattgggcga gaaaactttc cttgacctca tggttccacg cggcgggata agtcaccatg
     5461 atgtgccgca ggcggcgcgg cagttccgcg taaccggtcg aatcgagata tttgcggctg
     5521 ttgatctggc gatacgcggc ctcaatgaga gaaagcgcga accagcaaac agcgtcgcgg
     5581 cgcggatagc ttggcgggcg ttcgggataa cgacgcagga aatcttcgtc tccgggcgga
     5641 ttctcgatgt cccaatcgtg cgactcgggg tccatgtaaa cgcgtatcat gccgccaagt
     5701 tcagtcaaca tgctgccgcg tttgtcgggg tcgctcggat ttttgacctg catccaaaaa
     5761 actccgccct ggctgccggt ctgggcatca tcccaaacat atcgtttagg cgagctgaaa
     5821 taataacggc cgctcatcaa ggccaggtcg gaaagacgtt tggcgacgcc tttgttcgat
     5881 ttgccgccgc caaccatcgc cggagaaatc tcaatgaacg aacgcggcag ataacgcttg
     5941 cgccgataat aagttttgcc ggacggatcc tcataggatt cgtacatgtc gaaaagttgg
     6001 gagaggctga ggttcggctc gagcgccgcg aaagtggtgc gatgcatgag cagccacgag
     6061 gggatgattt cgcagtcctt gtccaccttg tcgtattgaa cgccgggcgg cgtgacgcgg
     6121 aggactttca cacggcgcga aaacggccat tggtccttgt cgggcgcacc gcgggtttcg
     6181 agcagcaatg cggcggtgcg ggtgttgccg aggtcaacga ccaggtccac gtcgatggga
     6241 tcgttgccgg cgggcttggc gaaaacgatg tgcaaatcaa ccggcaattc cttgccctcc
     6301 ttgttcggca acgagaagag cggcactgaa cgttcatcgc cgaggttctc cgtgatttgc
     6361 gcggcgtcgt cggaagcgta gggaaagaga aaatcctcgt tgcctttttg gccgagcttg
     6421 gaatcaattt cccacaacaa gccgttgcgg ggaatgctca tgttggtctc gactgcgatc
     6481 ttgattaccg gaacggacgt ggtggtgccg gaagtggaac cgaggtcggt ggattcgtcg
     6541 tattgataac gcacccaggg ataagggtcc cagacctgcc acgggtcgtt gatatgtttc
     6601 atcaccaagg tgttcaacgc cccgatgtcg cgattgggac agagcttctc cagttccagc
     6661 gtgaaatact ggtggccggt gttcgggaaa acttttagtt caatgggcat cggcgttcgg
     6721 gagtgatgat tgcggaatgc ggagtgcgga atgcctgaaa tcgtatcccc gggaatttct
     6781 taaatggcga accccctcac ccgaaccctc tcctccaagg gggcgaggaa gaatatgcct
     6841 ggaatttttt gtgtgggcgc tcattttttg gattcgtcgg gagattgcgc gcaagcgggc
     6901 aggcaatcgt tgtccatgca gagtttgcgg agttgttcgt cgccgggttg ggcctgacgc
     6961 tggatcaagc cgatgccttc attcaaaagt tttttcaact gcgcttcaag gaaaggttgc
     7021 aggaaaactt tgtaagctgg acacccacgg cctttcagcg tgcgaccttt ggggtccacc
     7081 gaaatatcat cgaagctggc gctgtcgccg atcccggaat cgtaagacgg agggccgctt
     7141 tttccataaa tcagcacgtt gcccatgcga atggccagga tacggcgcaa atcggtcccg
     7201 ggacggtcaa gattatattc caccaaatct tttagccaga cggccatcgg caagagatgg
     7261 ccctccaatg aagccacgag cgcttcaagc cagcttcgca atttctcccg cgaatccaat
     7321 ccgagcagcg accagtcggg tttgtcgcgg cggccgtttt tgcgccacgc ctcg
//
LOCUS       DASZMM010000271         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5325309, whole genome shotgun sequence.
ACCESSION   DASZMM010000271 DASZMM010000000
VERSION     DASZMM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5325309"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VG754_06545"
     CDS             complement(<1..601)
                     /locus_tag="VG754_06545"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWF18908.1"
                     /translation="MKIGVIGCGAVGSYYGAKLGHSGQEVHFLLRSDYETVRRKGVLV
                     RSAEGDFRFNPKCATTSEQIGKCDLVLIALKTTANDQLAKLLPPMVGESTAVLTLQNG
                     LGNEETISKLTGAEKVLGGLCFVCLNRIEPGVIQHVAHGAIVMGEHKRWPEPRTHDIA
                     GAIRHSGVPCKVTDSLERAHWEKLVWNVPFNGLGVASAAG"
     gene            complement(692..979)
                     /locus_tag="VG754_06550"
     CDS             complement(692..979)
                     /locus_tag="VG754_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWF18909.1"
                     /translation="MAKALSKSQVAAAIADKNSLSKKQAAEILDSIAELAYKNAKNTF
                     TLPGLGKLVLVNRKARVGRNPATGESINIPAKRVVKFRVAKAAKDAILGAK"
     gene            complement(1209..1772)
                     /gene="msrB"
                     /locus_tag="VG754_06555"
     CDS             complement(1209..1772)
                     /gene="msrB"
                     /locus_tag="VG754_06555"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWF18910.1"
                     /translation="MIFLLILGALGIGVALAAFATDSNSVSKADAGKVPIVEVRLLDD
                     KGQLGPKTAVPRVIKSDAEWKKQLTPEQYKIARAKGTEPAFCGLLLDEKKPGVYYCVC
                     CQLPLFSSNSKFHSGTGWPSFFAPIAPENVVTHVDQSYGMTRTEILCARCDAHLGHVF
                     DDGPRPTGLRYCLNSASLVFKEQKQSP"
     gene            complement(1799..2428)
                     /gene="msrA"
                     /locus_tag="VG754_06560"
     CDS             complement(1799..2428)
                     /gene="msrA"
                     /locus_tag="VG754_06560"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWF18911.1"
                     /translation="MKFVSAIIALFLLLSFTCAAEDAKPANTNMNSTVPSIPAGDESI
                     ILGAGCFWCTEAVFQQIPGVVSVTSGYIGGTVKHPTYEQICTGTTGHAEASRIVYDPK
                     KTDLEKILAVFWEAHDPTSLNRQGADSGTQYRSAIFYNTDEQRKIAEKSKTEAAKEFS
                     KPIVTEITKAGEFYPAEDYHQDYYRLNKNKNPYCRMVISPKLKKLGLKE"
     gene            complement(2550..3053)
                     /locus_tag="VG754_06565"
     CDS             complement(2550..3053)
                     /locus_tag="VG754_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18912.1"
                     /translation="MKKTALLLAACLWAGLSHAALQPGDSLTPYTIHNVSTGKEYCQV
                     CAYGAKPAKVVAFGKLHDAAFWADLEKLQIIASANPKLGVFAQVIDAKDSDSDAIKAE
                     AAKHGISFPVVVAVEKDWNKKYHVNGQSRVIYYAQQKNNITWTSVGLDGKAADELESQ
                     VKKDLAS"
BASE COUNT          832 a          981 c         1001 g          891 t
ORIGIN      
        1 aacctgccgc acttgccacg ccaagtccgt tgaacggcac gttccaaacc agcttttccc
       61 aatgcgctcg ttcgaggctg tcggtcactt tgcaaggcac accggaatgt cgaatcgcgc
      121 ccgcaatgtc atgagtgcgc ggttccggcc agcgtttgtg ctcacccatc acaatcgctc
      181 cgtgcgcgac atgctggatc acgccgggtt cgatgcgatt caggcaaaca aagcaaagac
      241 cgccgaggac tttctccgca ccggtcaact tcgagatggt ttcctcgttg cccaaaccat
      301 tctgcaacgt caacaccgcc gttgattcgc ccaccattgg cggaagcaat tttgccaatt
      361 ggtcgttcgc cgtcgtcttc agcgcaatca aaacgaggtc gcattttcca atctgctccg
      421 acgtcgtggc gcacttggga ttgaagcgaa aatctccttc ggcgctccgg accagcacgc
      481 ctttgcggcg caccgtttcg taatccgagc gcagaaggaa atgaacttcc tggccggaat
      541 ggcccagctt cgcgccgtaa taactgccga ccgcgccgca gccaatgacg ccgattttca
      601 tgggtgaatg tgcgtttccc gatgcatcga tgatgggaat gaaaaaggca ctctgaaatt
      661 tcagagtgcc ttgtcgaaaa ttgacggcct cttactttgc gccgaggatc gcgtccttgg
      721 cggccttggc gacgcggaac ttcacgacgc gcttggcggg aatgttgatg gattcgccgg
      781 tggcgggatt acggccgacg cgggccttgc ggttgacgag cacgagcttg cccaaaccgg
      841 gaagggtgaa agtgttcttg gcattcttgt aggcgagttc ggcaatggaa tcgagaattt
      901 cggcagcttg ctttttgctg aggctgttct tgtcagcgat tgcggctgcg acttgagatt
      961 tagacagtgc ttttgccata taatgttgtt tggttgttgt tagttttatt ttctaaaagt
     1021 cgttcgactt gggcgtatta aagccgttca agaagaccgc gcaagcagaa aattacaata
     1081 tttacagggg tgcgagaggg gttggcacgg acagtgcaag caaaattttc gcaattcttt
     1141 tggcgatgtc cactatagta ttccatgaaa atctgcgttc gtttcgtcat gcaaaaacgc
     1201 aacaggcgtc acggcgactg cttttgctcc ttgaacacca gcgacgccga gttcaagcag
     1261 tagcgcaacc cggtcgggcg cggaccgtca tcgaacacgt ggccaaggtg cgcatcacaa
     1321 cgcgcgcaaa gaatttcagt gcgcgtcatg ccgtaactct ggtcaacatg cgtcacgaca
     1381 ttctccggcg cgatgggcgc aaagaaactc ggccagcccg tgccggaatg aaatttggaa
     1441 ttcgaactga acaacggcaa ttggcagcag acgcagtaat aaacgccggg ctttttctcg
     1501 tccaaaagca atccgcaaaa tgccggctca gtccctttcg cgcgcgcgat tttgtattgc
     1561 tcgggcgtga gctgcttctt ccattccgca tcgctcttga tgacccgcgg caccgcggtc
     1621 tttgggccga gttgaccttt gtcatccaga aggcgcactt caacaatcgg gacttttccc
     1681 gcgtcggctt ttgaaacgga attcgaatcg gtggcgaacg cggccaacgc cacgccaatt
     1741 ccaagcgcac ccaatatcaa aagaaaaatc atatttcctc tccctttcat tcgcgccatc
     1801 actccttcag cccgagtttc ttgagctttg gcgaaatcac catccggcaa tacggattct
     1861 tgtttttgtt caaccgataa taatcctggt gataatcctc ggcgggataa aattcgcccg
     1921 ccttcgttat ctcggtcacg atcggtttcg aaaattcctt cgccgcttcc gtcttcgatt
     1981 tctccgcaat cttccgctgc tcatccgtgt tgtaaaagat ggccgaacga tattgcgtgc
     2041 cgctgtccgc gccctggcgg ttcaatgaag tcgggtcatg cgcttcccag aacacggcga
     2101 gaattttttc gaggtcagtc tttttcgggt cgtaaacaat ccgcgaagcc tccgcatggc
     2161 cggtcgtgcc ggtgcagatt tgttcgtagg tcggatgctt gaccgtcccg ccgatgtaac
     2221 cggacgtcac agaaaccacg cccgggatct gctggaacac cgcctcggtg caccagaaac
     2281 aacccgcgcc aaggatgatg gattcatcgc cggcgggaat ggacggaacg gtggaattca
     2341 tattggtgtt tgcaggtttg gcatcttcag ccgcgcaagt gaatgacagc aggagaaaca
     2401 acgcaatgat ggcagaaaca aatttcatgt aataaatgaa caatcatcgt aggaaccgcc
     2461 gtgaggaggc cctcgttaga tttgaaatat caaatttcaa atttgaaatc caaacagagc
     2521 ctccttacgt cggccctgca caatatgatt taactcgcga ggtccttctt cacctggctc
     2581 tccaattcgt ccgcggcttt gccatcaaga ccaaccgatg tccaggtgat gttgttcttc
     2641 tgctgcgcgt aataaatgac gcggctctgg ccgttcacgt gatatttctt gttccaatcc
     2701 ttttccacgg cgacgacgac ggggaagctg atgccgtgct tggcggcctc ggctttgatg
     2761 gcgtcggaat cggaatcctt ggcgtcaatc acctgcgcga acacgcccaa tttgggattg
     2821 gcgctcgcga tgatctggag cttttccaga tcggcccaga acgccgcatc gtgcaacttg
     2881 ccgaacgcaa ccaccttggc cggctttgcg ccgtaagcgc agacctggca atattccttg
     2941 ccggtcgaaa cattgtggat ggtgtaaggc gtgaggctgt cgcccggctg caacgccgcg
     3001 tgactgagac ccgcccacaa acaagctgcc aggagaagtg ccgttttttt catatcaatg
     3061 aataggatga ttgtatggtt aaactgttcc ctgccattct agtcgaaagg cggttttgcg
     3121 caagggcagt gacgttgaac gcaaaggcgc aaaggcgcga ggacgcaaag aatggaagct
     3181 aattgagcgg gcgtcccgat tgcgtttgtt gggtgtccat gctttagcat gtttgttctc
     3241 tttgcggctt tgcgcctcgg cgcctttgcg ttgaaaatgg cttgacttgc cttgactcgg
     3301 tttggactga aaataggata aagcattgat ggcgagtagg ttgattcgat tttcgcaagg
     3361 cttgaccaag gcttgactcg gggcacggaa tggagtgttg gagtattggt gaaaggcgca
     3421 tggtttgatc gccaagcgac atgaagcgac acagcaggct caattcggaa aacaccgaac
     3481 aaccaacgct gaacatcgaa tccttccggt cacattcagc gttcgatgtt tgagcccctc
     3541 cccccggcat tttcggcggg ttaaagcggg tttaagtgat tctaagcggg atttcaagta
     3601 cttatgcatg gaatggcgac gcgaggatga aggatggcac agatggcgac tttgaacctt
     3661 gaacttggct cgcaaaaacg ggaaacgcgg gaaatgcggg atacg
//
LOCUS       DASZMM010000272         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5325837, whole genome shotgun sequence.
ACCESSION   DASZMM010000272 DASZMM010000000
VERSION     DASZMM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5325837"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="VG754_06570"
     CDS             <1..1411
                     /locus_tag="VG754_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HWF18913.1"
                     /translation="VLKPDKLDYDQYREIRFRRDQALWASEKLPFEIEFFHPGYLYEE
                     PVHVNEFSPNYVQTIRFVQDFFDYGKLHIKSQIPANTGYAGFRVLYPLNVTNRWDELG
                     AFLGASYFRLLGKDQHYGESARGLALDCGESDRPEEFPIFTDWWLGKPQPEDKNLHMF
                     AILDSVSCAGAYEFHIQPGETTMAEIEAIIYLREPDKVHAVDAQKKPLATIGVAPLTS
                     MFWFGKNSERKFDDYRPEVHDSDGLLMRMDNGETLWRPLDNASQMRHQRFNAKDIRGF
                     GLLQRERDFTCYQDLFNPYQSVPSVWVEPKGHWGDGDIHLVELTTHYEGLDNIVAFWE
                     PKTKPAPLQPYHIAYTLYWTRETDRKLSTNKVIATRVGADSRNPQQRLFVIDFAGPKL
                     SALPQGTAPQAIPSCSDNATIVENQIFPMPPGTNGVWRTIIKMEPKAGNVNPVDIRCT
                     LKKGDDVLTETWTYHWSPP"
     gene            1384..1989
                     /locus_tag="VG754_06575"
     CDS             1384..1989
                     /locus_tag="VG754_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18914.1"
                     /translation="MDLSLEPSLISTSEGHDQPGLMAGRPLEEWNAAYGKVESYFHAL
                     RVRNRVLLGRLVIHVLKRAMRRAPHEPERSATALAAEEMDRVVTEWFAEVLQEPPTGT
                     DQMLSTRGRLALLLADMPGKWQDQFLRPGPWPHDFVSAMRRAYLQAGPDFQLSQMTPR
                     PMHFGPFTTLTNFTRLPYRKMILIWLLFVAGLILIFVLTHQ"
     gene            1993..4173
                     /gene="mdoH"
                     /locus_tag="VG754_06580"
     CDS             1993..4173
                     /gene="mdoH"
                     /locus_tag="VG754_06580"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HWF18915.1"
                     /translation="MDTDVIEVAGARNEEKHSQPQVMPVSHSGGGWRVFIFFSSAVLL
                     TGAVSLLFADLLWRTGWSTSRTFLLVLFVLLFFFSAIGCMNGFFGFILRRFGDPKCIK
                     KLADYRSKSIEGTSTAIIFPIYNEEVLRVYEGLRATYESLEKTGQLERFDFFVLSDST
                     NPDKWVEEERRWHDVVRELGALGRIYYRRRVANEGKKSGNVRDFLNNWGRRYRYFIVF
                     DADSVMRGETIVDLVKMMEANQDVGLIQTVPALMNAESLFGRIQQFANRFYTPIFIAG
                     LNFWSQGCGNYWGHNAIIRTEPFMQYCDLPQLPGRKPFGGQILSHDFVEAALLLRANW
                     QVWLASDLEGSYEEAPQGLIENAQRDRRWCQGNLQHGLVLFARGLRGLSRLHLLQGIF
                     GYLAGPLWFLFLITFCWMWGVQRFSGLTPITVNSWIMAHFSLSGSQHALLIFMICMGV
                     LLLPKVLSLLDVALDRERRRGFGGLPRATASTVVETLFSTLHAPLQMLWHLRFVITIL
                     LGIGVHWGPQKRTADGTAWSYALRRHWGHTLTGLVWGGLIWWLAPSMFWWFVPVFAGM
                     ALAIPVSVWTSRSSWGARVRSVGLFLTPEETTPTPELDTLRVRMVTMGGVGESKPLPH
                     DAGLTEVILDPYVNAIHVSMLREKKLNPNYAESLARLGAGTPVVRTLGEKLLLEGPDA
                     LKPQDRILVMSDAETMSWLHRQAWLRPGETLAPWWSSAIRQYAR"
     gene            4403..>4895
                     /locus_tag="VG754_06585"
     CDS             4403..>4895
                     /locus_tag="VG754_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWF18916.1"
                     /translation="MSASKQSTPRGSPAKQRLLDAAATVFARDGLDGATTRAIAQEAR
                     VNEVTLFRLFRSKENLISAVVGRTFDAPKAAPKISLPSETGNLRKDLFNFARLYESIL
                     TENLPLIRTLIGEIHRHGCHERKVADGIFRPLWTELIARFTRAQEQGKLRADVSPVIT
                     ADLL"
BASE COUNT         1043 a         1361 c         1388 g         1103 t
ORIGIN      
        1 cgtgctcaag cccgacaaac tcgattacga tcaatatcgg gaaatccgtt ttcgccgcga
       61 ccaggcgctt tgggcttcgg aaaaactccc gtttgagatt gaatttttcc atcccggtta
      121 cctttacgag gagccggtcc acgtaaacga attctcgccg aattacgtgc agacgatccg
      181 tttcgtgcag gatttcttcg attacggcaa gctgcacatc aaaagccaga ttccggcgaa
      241 caccggttac gccggtttcc gcgtattgta tcccttgaat gtgacgaacc gctgggacga
      301 attgggagct tttcttggcg cgagctattt ccgcctgctt ggcaaggacc agcattacgg
      361 cgaatctgcg cgcggcctgg cattggattg cggcgaatcc gaccggcccg aggaatttcc
      421 gatcttcacc gattggtggc ttggcaagcc gcagccggaa gacaaaaatt tgcacatgtt
      481 cgccatcctc gacagcgtga gctgcgccgg cgcgtatgaa ttccacatcc agccgggcga
      541 aaccaccatg gccgagatcg aagccatcat ttacctgcgc gaaccggaca aggtccacgc
      601 tgtggacgcg caaaaaaaac cgctggcgac cattggcgtc gcgccgctga ccagcatgtt
      661 ttggttcggc aaaaactccg agcggaagtt cgatgattat cgtcccgaag tgcatgacag
      721 cgatggtttg ctcatgcgaa tggacaatgg cgaaacgctt tggcgtccgt tggacaatgc
      781 ctcccagatg cgccatcaac gtttcaacgc gaaggacatc cggggattcg gcctgctgca
      841 acgcgagcgt gatttcacct gctaccagga tttgttcaat ccctatcaat cggtgccgag
      901 cgtctgggtg gaacccaagg gacattgggg cgatggcgac attcacctgg tggagttgac
      961 cacgcattac gaagggttgg acaacatcgt cgcattttgg gaaccaaaaa cgaaacccgc
     1021 gccattgcaa ccctatcaca tcgcttacac gctttattgg acgcgcgaga ccgaccgcaa
     1081 gctttccacg aacaaggtga ttgccacccg cgtcggcgcg gattcccgca atccgcagca
     1141 acggttgttc gtgatcgatt tcgccggtcc aaaattgtcc gccttgccac agggcaccgc
     1201 cccgcaggcc atccccagtt gcagcgacaa cgcgacgatt gttgaaaacc agatctttcc
     1261 gatgccgccg ggcaccaacg gcgtctggcg gaccatcatc aaaatggaac ccaaagccgg
     1321 caatgtgaac ccagttgaca tccgttgcac actcaagaag ggtgatgatg tgctgaccga
     1381 aacatggact tatcactgga gccctcccta atctccacga gcgaaggcca cgaccagccc
     1441 ggtctgatgg ccggaagacc gctcgaggaa tggaatgccg cctacggaaa ggtggaaagc
     1501 tactttcacg ccctgcgcgt ccgcaatcgc gtgctgttgg gacggctggt catccatgtc
     1561 ctcaaacgcg cgatgcgccg ggcgccccat gaaccggaac gctctgccac ggctttggcg
     1621 gcggaggaga tggaccgcgt cgtgaccgaa tggttcgccg aggtgttgca ggaaccgccc
     1681 accgggacgg accagatgct ttcaacgcgt ggccgcctgg cgctgcttct ggcagatatg
     1741 ccgggaaaat ggcaggacca gtttttgcgt cccggcccat ggccgcatga tttcgtcagc
     1801 gccatgcgcc gggcttatct tcaagccgga ccggacttcc agctctcaca gatgacaccg
     1861 cgtccgatgc attttgggcc tttcaccacg ttgacgaatt ttacccggct gccttaccgg
     1921 aaaatgattc tcatctggct tctgtttgtg gcgggattga ttctcatatt cgtgctcacg
     1981 catcaatgag ccatggacac cgacgtcata gaagtggcag gagcccgcaa tgaagaaaaa
     2041 cattcccaac cgcaagtgat gcctgtttca cattccggcg gcggatggcg cgtgttcatc
     2101 tttttttcca gcgcggtgtt gctgaccgga gcggtttcgc tgttgttcgc cgatctgctt
     2161 tggcgcaccg gttggtccac ttcccgcacg tttttgctcg tattgttcgt cctgctattt
     2221 ttcttctcgg ccatcggttg catgaatgga tttttcggtt tcatcctgcg ccggttcggt
     2281 gatccaaaat gcatcaagaa acttgcagat tatcgttcca aaagcatcga agggacaagc
     2341 acggccatca ttttcccgat ttacaatgaa gaagtgctgc gggtttatga aggattgcgc
     2401 gccacgtacg aatcgctcga aaaaaccggg caactggagc gcttcgattt ttttgtgctg
     2461 agcgactcga ccaatcccga caaatgggtg gaggaagaac gccgctggca cgatgtcgtc
     2521 cgcgaacttg gcgcgttggg ccgcatttac tatcgccgcc gcgtcgcaaa cgaaggaaag
     2581 aaaagcggca acgtgcgcga tttcctcaac aattggggaa gacgctaccg ttatttcatc
     2641 gtgttcgatg ccgacagcgt tatgcgcggc gagacgattg ttgatttggt gaaaatgatg
     2701 gaggcgaacc aagatgtcgg cttgatacaa accgttccgg cgctcatgaa cgctgagtca
     2761 cttttcgggc gcatccagca atttgcgaat cgcttctaca cgccgatttt catcgccggg
     2821 ttgaatttct ggtcgcaagg ctgcggaaat tattgggggc acaatgccat catccgcacg
     2881 gagccgttca tgcaatattg cgacctgccg caattgcccg gccgcaaacc gttcggcggg
     2941 cagattctca gccacgattt tgtggaagcc gcgctcctgc tccgcgcaaa ctggcaggtc
     3001 tggcttgcaa gcgaccttga aggcagttac gaggaagcgc cgcaaggact cattgaaaac
     3061 gctcagcggg accgtcgctg gtgccaggga aatctgcaac acggattggt tttgttcgcg
     3121 cgtggattgc gcgggctcag ccggctgcat ctgttgcaag gcatcttcgg gtatctcgcc
     3181 ggaccgcttt ggtttttatt tctgatcaca ttctgctgga tgtggggcgt ccaacggttc
     3241 agcggactca cacctatcac cgtcaacagt tggatcatgg cgcacttcag cctcagcggc
     3301 agccagcacg cgcttctcat tttcatgatc tgcatgggcg tgttgctgct gccaaaagtg
     3361 ctttcgttgc tggacgtcgc gctggaccgc gaacggcggc gtggtttcgg cggattgccg
     3421 cgcgcaaccg caagcacggt tgtcgaaacg cttttttcca cgttgcacgc gccgttgcaa
     3481 atgctctggc atttgcgttt tgtgatcacg atcctgctgg gcattggcgt gcattggggt
     3541 ccgcaaaaac ggactgcgga cggcacggcg tggtcttatg ccttgcggcg tcattggggg
     3601 cataccttga ccgggctggt ctggggcgga ttgatttggt ggcttgcgcc ttcgatgttc
     3661 tggtggttcg tgccagtttt cgcggggatg gcgctggcga ttcccgtgag cgtatggacg
     3721 agccgcagca gttggggtgc gcgtgtgagg tcggtcggtc tttttttgac gccggaagaa
     3781 acgacgccga caccggaatt ggacacattg cgcgtgcgaa tggtcacaat gggcggcgtt
     3841 ggcgaatcaa aaccacttcc gcacgatgcc ggcctgaccg aagtcattct cgacccttac
     3901 gtgaatgcaa ttcacgtctc aatgttgcgc gagaaaaagt tgaacccgaa ttatgccgaa
     3961 tccctggcgc gattgggggc cggcacaccc gtagtgcgca cgctcggaga aaaattgctg
     4021 ctggaaggcc cggacgcttt gaaaccgcag gacaggattt tggtgatgtc cgacgcggaa
     4081 acgatgtcat ggcttcatcg gcaggcgtgg ttgcgtccgg gcgagacgct ggcaccctgg
     4141 tggagttcag cgatccggca gtatgcgaga taaacccaat cactttatgc caaaaaagat
     4201 ttcgaaattg accaaacgcc ggcaaaagcc acgcagaaca ttggttgagt atcttgacaa
     4261 atttgccggc cttccagtgc gtaacaggga aacggttcca aaatcgaagt tttaatcaga
     4321 aattcgccgt cgattcgcga aggcatactt ttacctattg ctttcgactc ctgcgtggca
     4381 tagtatgcaa gcaagtgctt gcatgagtgc atcaaaacaa tcaacaccgc ggggttcgcc
     4441 ggcaaaacaa cggttgctgg atgccgctgc cacagtcttt gcccgtgacg ggctggacgg
     4501 ggccacgacc cgcgcaatcg cgcaagaagc gcgcgtgaat gaagtcacgc tttttcggct
     4561 gttccgttcc aaggaaaacc ttatctcggc agtggttggt cgcacctttg acgcgcctaa
     4621 agccgctccc aagatttcct tgccgtccga aaccggcaat ttgcgcaagg acctttttaa
     4681 ttttgcccgg ctttacgagt ccattctgac ggaaaacctg cccttgatac gcacactcat
     4741 cggggaaatt caccgtcacg gttgccatga acggaaggtt gccgatggca tcttccgtcc
     4801 gctttggact gaattgatcg cccggttcac acgagcgcag gaacaaggca agctccgggc
     4861 cgatgtttcc ccggtgataa cggccgatct gctgg
//
LOCUS       DASZMM010000273         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2580397, whole genome shotgun sequence.
ACCESSION   DASZMM010000273 DASZMM010000000
VERSION     DASZMM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580397"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VG754_06590"
     CDS             complement(<1..285)
                     /locus_tag="VG754_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18917.1"
                     /translation="MKTPRSSILLTLCLVLGCNDKTPKTAGTTNAPGSKPLNAPADYL
                     GGMADGRNRAVKTVDTASLTQAIQMFNVDHGRYPKDLSELVQEKLISKIPD"
     gene            complement(295..465)
                     /locus_tag="VG754_06595"
     CDS             complement(295..465)
                     /locus_tag="VG754_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18918.1"
                     /translation="MLKIVTAMMLARIMGIITIITILRTTRLQCLIQVRNTTPASTVV
                     RIIRIEIGEFCW"
     gene            complement(1272..>2586)
                     /locus_tag="VG754_06600"
     CDS             complement(1272..>2586)
                     /locus_tag="VG754_06600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWF18919.1"
                     /translation="ARRRLALDEFITLQKDIRLRRQKFETKARGLPCAGNNRLIKPFL
                     AQLGFALTTAQTKVLRELRHDMGGVHPMRRLLQGDVGSGKTVVAACCALMALESSFNV
                     ALMAPTEILAEQHYQTFKQWFEPLGIQVQLQTGTHKSEIRNPKSPTFTIGTHALIESN
                     FSLDRLGLVIIDEQHKFGVTQREQLLRKGTYPHLLVMTATPIPRTLGLTLYGELDISV
                     IDELPPGRGQIKTFVRAADKLPKVWDFIRDKIAQGRQAYVVYPRIEDGGAAGLKAVTK
                     EFENLKALLAPHRVGLLHGRLSGAEKERVMTAFRANAIQVLLATPLIEVGVDIPNATV
                     MLIENAEQFGLAQLHQLRGRIGRGAHNSYCVLVAAAKSAESRQRLKVLEETNDGFRVA
                     EADLKLRGPGDLLGQQQSGLPKFRFGSLTEDLDLIQLARELAGQI"
BASE COUNT          571 a          746 c          699 g          570 t
ORIGIN      
        1 atcgggaatt ttggagatca acttttcctg cacgagttcg ctgaggtctt ttggatagcg
       61 gccatggtcc acgttgaaca tttggatggc ctgtgtcagc gacgcggtgt caaccgtttt
      121 gaccgccctg ttcctgccat cggccatccc gccgaggtaa tctgccgggg cattcaacgg
      181 cttgctgccg ggcgcgttgg tcgtcccagc ggtcttgggc gttttatcgt tgcaacccaa
      241 cacgaggcaa agcgtcaaca atatggacga gcggggcgtt ttcatgttgc aaacctacca
      301 gcaaaattcc ccaatctcaa tgcgaatgat gcgaaccacc gtcgaagccg gagtggtgtt
      361 gcgcacctgt atcaggcatt gaaggcgcgt ggtgcggagt atcgtgatta tggtgatgat
      421 gcccatgatg cgcgccagca tcattgccgt gacgatcttg agcatttggt tcggagttcc
      481 gcgatgcgtc aaaagcgtct gtgttcaacc agaaggaatc ggaagcagac ctgcggcgag
      541 aacgcgaacc gctccgcgtg gcaaagatga gcgcaaatat gattatgggg agggccgctg
      601 caatgccaat tggaatcaaa atgtccatgg ttcacctttc aggctgaagg tgttctaacc
      661 atgacaatgc gtcaattcat ttatcacaag ttatggccaa aagggcggat ctgactattt
      721 tgcacgtttc tgggctcttg cgaattgtgt tcatgggccg gcgcacatct ctgtccccct
      781 caccccagcc ctctccccaa ggagaggggg ccatttggcc gccgctcgat aaatcgaacg
      841 ctctcggttt gcaaccacga cgggatgcgt tgctccctct ccccaaggga gagggccggg
      901 gtgaggggga gggaatgttc aaccactccg ttgtgcaaga aactgagatg cgccctggcc
      961 aaaagccgat tctcctccag accaaaaatt ccggatacag aaaaaagaaa tccacgccgc
     1021 gcgagttcgc gcggttccgg aactggattg cagcctatct tacttgttcg tcgagggtgc
     1081 agccggcgca ttggtggcgg gctgcgtgct cgtcgtgtta tccgacggac tgcaacccac
     1141 caacacgaaa ccactcagta cgcacaataa caacacaaga gatgttttcc ttttcatatc
     1201 gccatggtac cacaggtgaa aagaaatacc agtgggaaaa caggaaactt tgcgcgttca
     1261 acacccgatg tttaaatctg tcccgccaat tcgcgcgcga gttggatcag gtccaaatcc
     1321 tcggtcaaac tgccaaaacg aaatttcggc aaaccgcttt gttgctggcc gagcaagtcg
     1381 ccgggtccgc gcaatttcaa gtccgcctcc gccacccgaa aaccgtcgtt cgtctcttcc
     1441 aagaccttga gacgctgccg cgactcggcg ctctttgcgg cggcaacgag gacgcaatac
     1501 gaattgtgcg cgccgcgtcc aatgcgcccg cgcaactgat gcaattgcgc cagcccgaat
     1561 tgctcggcat tttcaatcag catcactgtc gcatttggaa tgtccacgcc cacctcgatc
     1621 aacggcgtgg cgagcaacac ttggatggca ttcgcgcgaa acgccgtcat cacgcgctcc
     1681 ttttccgcgc ccgacaaccg tccatgcaac aatccgacgc gatgcggcgc gagcagcgcc
     1741 ttcaaatttt caaattcttt cgtgaccgcc ttcaatccag ccgcaccgcc gtcttcaatc
     1801 cgcggataaa cgacatacgc ctgccggcct tgcgcaattt tgtcacggat gaaatcccat
     1861 accttcggca atttgtccgc cgctcggacg aatgttttga tttgtccgcg ccccggcggc
     1921 aattcgtcaa tcacggaaat gtccaattcg ccatacaacg tcagccccaa tgtgcgcgga
     1981 atgggcgtcg cggtcatgac cagcagatgc ggataagtgc ccttgcgcaa caactgctcg
     2041 cgttgcgtca cgccgaattt gtgctgctcg tcaatgatga ccaacccgag ccggtcgagc
     2101 gaaaaattag attcaatcag ggcgtgcgtg ccgatggtaa aagtcggcga tttcggattg
     2161 cggatttcgg atttatgtgt cccggtttgc aattgcacct gaatacccag tggctcgaac
     2221 cactgtttga aagtctgata atgttgttcg gcgagaattt ctgtcggtgc catgagcgcg
     2281 acgttgaacg agctttccaa ggccatcaac gcgcagcacg cggccacgac ggttttgccc
     2341 gaaccgacat cgccctggag caatcgccgc atcggatgca cgccgcccat gtcgtgccgc
     2401 aactcgcgca gcacctttgt ctgcgcggtc gtgagcgcga aacctaattg cgcaaggaac
     2461 ggtttgatga gccggttgtt gcctgcgcaa ggcaagccgc gcgctttcgt ctcaaatttt
     2521 tggcgacgca gccggatgtc cttctgcaac gtgatgaact catccagcgc gaggcgacgt
     2581 cgagca
//
LOCUS       DASZMM010000274         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2309380, whole genome shotgun sequence.
ACCESSION   DASZMM010000274 DASZMM010000000
VERSION     DASZMM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309380"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VG754_06605"
     CDS             <1..368
                     /locus_tag="VG754_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HWF18920.1"
                     /translation="REKYRGIRPAAGYPACPDHTEKGLLWQLLDAEKSAGIKLTESYA
                     MWPASSVSGLYFAHPEAKYFGVGKIDRDQVLDYHLRKQMDLQTVERWLAPNLNYEPDD
                     TAKSLNETKIGCACGIPHG"
     gene            561..1649
                     /locus_tag="VG754_06610"
     CDS             561..1649
                     /locus_tag="VG754_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18921.1"
                     /translation="MKHTRIPALLSGIFLLGAFVVNLHAQAPASIAGDAIMAHATGGT
                     GTFSKGGYYLFLPADSGSDYQLIGADGISSSSGTYAYSSSDAVGTIDFSDSNMGAGTN
                     SITFQPDLTGQFTLTAGTHEQTGSLVLATGPALPSLTNHSYFLNILAGSTPFVSQGSV
                     IFTTGASGSNYTVTPTSGGIANSSGTYAYTTVNASTGFFSVNDDDSGSSSEYFAFTNS
                     GGGVYLVTETSGAFQTGTFTVSNLIPTMFFMGQIDLASHVEWVQFSDGAPFGYYDVMD
                     FGFPCFYHFDLGFEWYFDANNSSHGCYLYDFSSDTFFYTAPDVFPYLYDFTLNAWLYY
                     FPDPNAQDHYSHNPRYFYNFGTKQIITK"
     gene            1755..2207
                     /locus_tag="VG754_06615"
     CDS             1755..2207
                     /locus_tag="VG754_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HWF18922.1"
                     /translation="MKNEVVPVQIRGILPANSGCALFVGNDSKVFVINVEPAMGQVIG
                     MFLRETPKERPLTHDLIANIFKGFSISVERVVITELKNSTYYARLILHQQNELARKIV
                     EIDARPSDCLAIATAQKKPIFVSAPLFEQVEDMTEVLDRINENGGELE"
     gene            2213..2752
                     /locus_tag="VG754_06620"
     CDS             2213..2752
                     /locus_tag="VG754_06620"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18923.1"
                     /translation="MPPSPKPLPPLVLVCGDDEFAVKQRAKEIYQKWCDELGGMDHEI
                     IDASASNSGEALKAISRVREALQTLPFFGSGKAVWLRDCNFLGDERAASTKAVTDSLA
                     ELAQELKEFSWSSVRLLISAGKVDKRKVFYKTVDKIGAVENFDGWSIDDRDWADQAEV
                     WARTALRDRKKEISDEALA"
     gene            2765..3352
                     /locus_tag="VG754_06625"
     CDS             2765..3352
                     /locus_tag="VG754_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18924.1"
                     /translation="MGPNARSLANEVEKLSLFIGDRPEIEVADVMTVCTRNKSARAFA
                     LGDALGDRDLPRLLRTLDEELWELKLDRQKSEIGLLYGLIGKVRAMILLKEMLREGWI
                     KPDGDYNRFKAQLERVPAAQLPEDKRYNPLALNAYVLFKALPQCRRYSQAELISAMEL
                     LLRCNQRLISSSLDEALILQQTLVEIVEKPAAAKR"
BASE COUNT          871 a          925 c          996 g          884 t
ORIGIN      
        1 tccgcgagaa atatcgcggc atccgtcccg cagccggtta cccggcgtgc ccggaccaca
       61 cggaaaaagg tttgctgtgg cagttgctgg acgccgaaaa aagcgccggc atcaaattga
      121 ccgagagtta cgctatgtgg ccggcgagtt cggtgagcgg actttatttc gcgcatcccg
      181 aggcgaagta ttttggcgtc ggtaaaattg accgcgacca ggtccttgat tatcaccttc
      241 gcaaacagat ggatttgcaa acggtggaac gctggctagc gcccaatttg aattacgagc
      301 cggatgatac cgccaagtcg ctgaacgaaa cgaaaattgg ttgcgcttgc ggaatcccgc
      361 atggttaagc tgcgaaggca ccgcgaactt ttgcggtctg atttcaaatc tcgataatcc
      421 tttgattcca ccccaggagg agcgcggaag accccccata ttcgcggact gcgagttgtg
      481 caaaaatcgc gtcgatggag ttgaagaaag tctccaaata ataacaatca gaagctgtaa
      541 ttagaaggaa gcatcctctt atgaagcaca cccgaatccc cgccttgctg agcggcattt
      601 ttttgctcgg cgcttttgtc gtcaacttac acgcgcaagc tccggcttcc attgcgggag
      661 acgccatcat ggcacacgca acgggtggaa cggggacttt ttccaaaggc ggttactatc
      721 tttttctgcc ggcagattcg ggatcggatt atcaattgat cggcgctgac ggcatcagca
      781 gcagcagtgg aacgtacgct tactcgtcgt cggatgcggt ggggaccatt gatttctccg
      841 attcgaacat gggcgcggga accaactcga tcacattcca acctgacttg acaggccagt
      901 tcaccttaac ggccggcaca cacgaacaga ccgggagctt ggtgctcgcc actggtccgg
      961 ctctgccctc gctcacaaac cacagctact tcctgaacat tttggctggc agcactccat
     1021 ttgtgagtca aggcagtgtg atttttacca caggggcgtc agggagcaat tacaccgtga
     1081 cgcccacttc gggcggcatt gcgaacagct cgggaacgta cgcttacacg accgtcaatg
     1141 cttccacggg atttttttcg gtgaacgacg atgattccgg cagcagcagc gaatattttg
     1201 cgttcacgaa ttccggcggt ggtgtttatt tggtgacgga gacgtcgggt gcttttcaga
     1261 cgggaacgtt cacggtttca aacctgattc caacgatgtt tttcatgggc cagattgatt
     1321 tggccagcca cgtggaatgg gtgcaattta gcgatggagc gccgttcggt tactatgacg
     1381 tgatggattt cggcttcccc tgcttttatc atttcgacct cggtttcgaa tggtatttcg
     1441 atgcgaacaa cagttcgcat ggttgttatc tgtatgattt ttcaagcgac acgttctttt
     1501 acaccgcgcc tgacgtgttc ccttacctgt atgatttcac cctcaatgcc tggttgtatt
     1561 atttccccga tcctaacgct caagatcatt attcccacaa tcctcgttac ttttataatt
     1621 tcgggacgaa acaaatcatc accaaatagc gggctgccaa acggccaaaa actccggttc
     1681 cacatcattt cccgcgcgca ggatggggat ttcaggagac ttgttttttc aaatttaagc
     1741 cataaactgt ggatatgaag aatgaagtcg tgcctgtgca aatccgtgga attttgcctg
     1801 caaacagcgg gtgcgccctc ttcgtcggga acgattcgaa ggttttcgtc atcaatgtcg
     1861 agccggcgat ggggcaggtc atcggaatgt ttcttcggga aacgcccaag gaacgtcccc
     1921 tgactcatga tttgatcgcc aatattttca agggtttcag catctcggtc gagcgggtcg
     1981 tcatcaccga actaaaaaat tcaacttatt acgcgcgtct gattttgcat caacagaatg
     2041 aactcgcgcg gaagatcgtt gagattgatg cccggccaag cgattgcctc gccatcgcca
     2101 cggcgcaaaa aaagcccatt tttgtttccg ccccgttgtt tgaacaggtg gaggacatga
     2161 ccgaagtcct cgaccgcatc aacgaaaacg gcggcgaact cgagtaacct tcatgcctcc
     2221 gtctcccaag ccgctcccgc ccctggtgct tgtctgcggc gatgatgaat ttgccgttaa
     2281 gcagcgcgcc aaggagattt atcagaaatg gtgcgacgaa ttgggcggca tggaccatga
     2341 aatcatcgac gcaagcgcat cgaacagcgg cgaagcgctg aaagcaatct cgcgcgtgcg
     2401 cgaggcgttg caaacgcttc ccttcttcgg cagcggcaag gcagtctggt tgcgcgattg
     2461 caactttctt ggtgatgaac gcgctgcatc gaccaaagcc gtgacagatt cgcttgcgga
     2521 acttgcgcag gagttgaagg aattttcctg gagcagcgtc cgtctgctta tcagcgccgg
     2581 caaggtggac aagcgcaaag ttttttataa aacggtggac aaaattgggg ccgtggaaaa
     2641 tttcgacggc tggtccatag acgacaggga ctgggcggac caggcggaag tctgggcgcg
     2701 aacagcgttg cgcgaccgca agaaagagat ttcggacgaa gcgctggcgt agctggttaa
     2761 taatgtgggg ccgaatgcgc gttcgctggc caatgaggtg gaaaaacttt cgttgtttat
     2821 aggcgaccgt cctgaaatcg aagtggcgga cgtgatgacc gtttgcacgc gcaacaaatc
     2881 cgcgcgcgct ttcgcattgg gcgacgcatt gggcgaccgc gatttgccgc gtctgctgcg
     2941 cacgcttgat gaggaattgt gggagttaaa gctcgaccgt caaaagtcgg aaatcggttt
     3001 gctttacgga ttgatcggca aggtgcgcgc gatgattctg ttgaaagaaa tgttgcgcga
     3061 aggatggatc aaacccgatg gcgattacaa ccgtttcaag gcgcaactgg agcgcgtacc
     3121 ggcggcgcaa ctgccggaag acaagcgtta caatcccctc gccttgaatg cctacgtgct
     3181 tttcaaggcg ctgccccaat gccgccgtta cagccaggcc gaattgatca gcgcgatgga
     3241 actgctcctc agatgcaatc aacgcctcat ttccagcagt ttggatgagg cgctgatttt
     3301 gcagcagacg ctggtggaaa ttgtggaaaa accggcggct gcgaagcggt gaatggtcaa
     3361 atccgttaca gagccatccc gtgggaaaat agaattagga aagcaggaaa agacgatgtt
     3421 tcctcaagaa ttttgttcct gatttcatga tttcctaatg aaacactaaa tctcattttc
     3481 tgccgttcat ctggttttgc ccataaaacc taaaatcggg atttaggtga catgagcatc
     3541 tcgccaaggt gactgatacc ttgtttttgt gaatgtgaaa aaatcgaata acgaacatcc
     3601 aatggaacgg tggcccgctt tctcattcga tgttccgcgt ccaatgttga gcgttggagg
     3661 ttttcttgag cgtcag
//
LOCUS       DASZMM010000275         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2581219, whole genome shotgun sequence.
ACCESSION   DASZMM010000275 DASZMM010000000
VERSION     DASZMM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581219"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VG754_06630"
     CDS             <1..895
                     /locus_tag="VG754_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWF18925.1"
                     /translation="DSRMNAGIKILEHTPEDLTATIEAGITLVALQNELARHRQWLSV
                     DPPHPKRLTIAELIDRNLSGPRRFGYGTVRDHLIGIKVALADGTIIHSGGKVVKNVAG
                     YDLMKLFIGARGSLGTVLETTFKLRPLPEMERFVQTRCETLEQADKAIEMVMHSDLAP
                     VVLDLVNAECGMRSAESGTMSSGIKVYSLVLGFAGTREEVEWQLERAKELGFDEPASL
                     EYEEKFWNETTPTHRMSVLPSKIVEALRSLNSPFVARAGNGVIYHGGRVTQRKEELPL
                     ELMRRVKAAYDPKNLLPQMPL"
     gene            892..2151
                     /locus_tag="VG754_06635"
     CDS             892..2151
                     /locus_tag="VG754_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWF18926.1"
                     /translation="MNTDAPERFLDYDKSLACIHCGLCLSSCPTYLETGNENDSPRGR
                     IYLMRAVQDGRLPLGGTAVRHIDLCLGCRACEAVCPSGVQYGDLLEHTRDHIEKHHRR
                     SPFQRFLRRVAIEKVFPFPSRMKLALLPAKAIRALRVEKLLPKFAREALSLIPDDAAE
                     VKLPSVSPATAPGGQRAGFISGCVMSVLFGKTNAASVRLLNRAGYEVVTPPEQVCCGA
                     LYAHSGQLELARDCARRNIDIFERQNLPLIVINAAGCGSTLKEYGQLLRDDPQWAERA
                     RQFSAKVKDLTQVLAKTLSSGNSALQTPHSELKKVTYHDACHLAHPQHITKPPRALVR
                     AVAGANYVELPESDVCCGSAGSYNLTEPEMAERLQRRKIENILKTGAQVVVTTNPGCL
                     LQIRAGLAKAGAKVEAMHIADFLDRVK"
BASE COUNT          470 a          616 c          626 g          439 t
ORIGIN      
        1 agatagccgc atgaacgcag gcataaaaat tctggaacac acgccggaag acctgaccgc
       61 gaccatcgag gcgggcatca cgctcgtggc gctgcaaaac gaattggcac gccatcgcca
      121 atggctgtcg gttgacccgc cgcatcccaa acgcctgacc atcgccgaat tgattgatcg
      181 aaatctcagc gggccgcggc gatttggtta tggcaccgtt cgcgatcatc tcatcggcat
      241 caaggtggcg ctggccgacg gcaccatcat ccattccggc ggcaaagtgg tgaagaatgt
      301 ggccggttac gatttgatga aattgttcat cggggcgcga ggaagccttg gaacggttct
      361 cgaaacgaca ttcaaattgc gcccattgcc ggaaatggag cggtttgtcc agacgcgttg
      421 cgaaacattg gaacaggcgg acaaggcgat tgaaatggtg atgcattcgg atttagcgcc
      481 cgtggtgttg gacttggtaa atgcggagtg cggaatgcgg agtgcggaat ctggaacgat
      541 gagttcggga atcaaagtgt attcgctcgt gctgggcttt gccggcacgc gcgaggaagt
      601 ggaatggcaa ctcgaacggg caaaggaact tggttttgac gaacccgcat cgctggagta
      661 tgaggaaaaa ttttggaatg aaaccacgcc gacgcatcgc atgtctgttt tgccgtcaaa
      721 aatcgtcgag gcattgcgta gtttgaattc gcccttcgtc gcccgcgcgg gcaatggcgt
      781 catttaccat ggcggccggg tgacgcagcg caaggaagaa ttgccgctgg aattgatgcg
      841 gcgcgtgaaa gcggcttacg atccaaaaaa tctgttgccg caaatgccct tatgaacacc
      901 gacgcgccgg aacgtttcct ggattacgac aaatcgctcg cctgcattca ctgcgggctt
      961 tgtctttctt cgtgcccgac ttatctcgaa acaggcaatg aaaatgattc gccgcgcggc
     1021 cgcatttatc tcatgcgcgc ggtgcaggat ggcaggcttc cgcttggcgg aacggcagtg
     1081 cggcacattg acctgtgcct cggctgccgc gcctgcgaag ccgtttgccc aagcggcgtc
     1141 caatacggcg acctgctcga acatacgcgc gaccatatcg aaaaacatca tcggcgctcg
     1201 ccgttccaga gatttttgcg ccgcgtggcc atcgaaaaag ttttcccgtt tccatcgcgc
     1261 atgaaattgg cgttgctgcc cgcgaaggcc attcgcgcgt tgcgcgtgga aaaactgctg
     1321 ccgaaattcg cgcgcgaggc gttgtccctc attcccgacg atgccgccga agtgaagttg
     1381 ccgagtgttt cacccgcgac cgcgccgggc gggcaacgcg ccgggttcat cagcggttgc
     1441 gtgatgagcg ttttgttcgg caagaccaac gccgccagcg tgcgtctgct caatcgcgcc
     1501 ggttacgaag tcgtcacgcc gccggagcaa gtttgttgcg gcgcgttgta tgcgcacagc
     1561 ggacagcttg aacttgcccg cgactgcgcg cggcgcaaca tcgacatttt tgagcgccag
     1621 aatctgccgc tcatcgtcat caacgccgcc ggttgcggtt ccacgctcaa ggaatacggc
     1681 cagttgctcc gcgacgatcc gcaatgggcc gaacgcgccc gccaattcag cgcaaaagtg
     1741 aaagacctga cccaagtgct tgccaagact ttatcttccg ggaattccgc actccaaact
     1801 ccgcactccg aacttaaaaa ggtgacttac cacgacgcct gtcatcttgc gcatccgcag
     1861 cacatcacca agccgccgcg cgctttggtc cgtgcggtcg cgggtgcaaa ttatgtcgaa
     1921 ctccccgaat ccgatgtctg ttgcggcagc gccggaagtt ataatttgac cgaacctgag
     1981 atggccgaac gcctgcaacg ccgcaagatt gaaaacattc tcaagaccgg cgcgcaggtc
     2041 gtcgtgacca ccaatcccgg ctgcctgctc caaatccgcg ctggcctcgc gaaagccggt
     2101 gcaaaggtcg aagccatgca catcgcggat tttttggatc gggtgaaatg a
//
LOCUS       DASZMM010000276         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3127024, whole genome shotgun sequence.
ACCESSION   DASZMM010000276 DASZMM010000000
VERSION     DASZMM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3127024"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VG754_06640"
     CDS             <1..439
                     /locus_tag="VG754_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18927.1"
                     /translation="PHDRFTRASLDETAKRFEVISSGWYEKDRLPVAWWPRYLLKKLS
                     RARHWRVGNARLLTHPGCLLSSHRPRATMLDAVKRAVRSAPLTVLVTHWWEYFPDRRP
                     DGEFIWQLHAVGDFLARERDIQVISFDDLTRADVAKRVFGQGC"
     gene            complement(480..800)
                     /locus_tag="VG754_06645"
     CDS             complement(480..800)
                     /locus_tag="VG754_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWF18928.1"
                     /translation="MKIRHQGETVCISDLKELALANAAGFAEEARSAMATGAKSIVVD
                     LSETGFLDCGGIGALIALRNNAQRDHHDISIRVVNPTPPVRRILQLTHLDNLLDSTPA
                     ELAA"
     gene            complement(797..1669)
                     /locus_tag="VG754_06650"
     CDS             complement(797..1669)
                     /locus_tag="VG754_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18929.1"
                     /translation="MKACIICPSPRAEVSALSQTVPLAAVPMLGKTLLEYWLEHLAGL
                     GATQVQVMAADRPEVVRHIVGDGARWGLRAEVLVEPRELSPGLARTKYPALQKSEWLP
                     DPYDMIAMLHLPGLPERPLFNSYADWFTAQMAWMPSTCRVNRIGLHETQPGVWVGARA
                     HIASDAVLCAPSWIGDDVTVGARSIIGPMTILENRAWVEADCEISGSFVGADTLVGRF
                     TELRESLAMGSTLVNWKTSSVATVDDPFILCSLGNAFPKLETLSWRQQLAAIFSRGKE
                     EADLLLDLKKMKLP"
     gene            complement(1670..2407)
                     /locus_tag="VG754_06655"
     CDS             complement(1670..2407)
                     /locus_tag="VG754_06655"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWF18930.1"
                     /translation="MRDTCGAEINDGLLRHYGLARSPWGRAELQIQLAFKRLCWHCRV
                     HSGPVVKRSFDLIVSLLLLLLFSPVLFAIGLLVKLEDGGPIIFAQTRVGRFGREFKMF
                     KFRSMCMDAEARLKDLIAKNRHGNGVTFKIQDDPRITRVGRWLRKFSLDELPQLYNVF
                     IGDMSLVGPRPPLPREVKLYSLADRRRLAAPPGITCFWQISGRSEIDFAGQVKLDVSY
                     IESQDFWMDVKILALTLPAVVRAKGAC"
     gene            complement(2655..>3243)
                     /locus_tag="VG754_06660"
     CDS             complement(2655..>3243)
                     /locus_tag="VG754_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9"
                     /protein_id="HWF18931.1"
                     /translation="TGLGWKRPQEIVHQYAQNQRRALPPDGIHIGNLQAGYSWISQYQ
                     NELTALSFPSDGDETAPYAFYDRWADTFNLTTEFVILNQGRSLAYLAWLMARTPLKNQ
                     PWRSADARIAFSPAENSSSRMLMATLESPGLDLQSARVVWEWQNHEPVWGKTVQLPLK
                     GGSRWIEAEAQLPDGRRVFAVTNLTSVAFSHFAKP"
BASE COUNT          732 a          884 c          948 g          679 t
ORIGIN      
        1 gccgcatgat cggttcacgc gtgccagctt ggacgaaacc gcgaaacgtt ttgaggtcat
       61 ttcatccggt tggtatgaaa aggaccggct gccagtggca tggtggccgc gttatctgtt
      121 gaaaaaactt tcgcgcgcga gacattggcg tgtcggaaat gcgcgtttgc tcacacatcc
      181 cggctgtttg ctttcgagcc accgtccccg cgccacaatg ctcgacgcag tgaagcgcgc
      241 cgttcggagc gcgccattga ccgtgctggt gacgcattgg tgggaatatt ttccggaccg
      301 ccggccagac ggtgaattca tctggcaatt gcacgctgtg ggagattttc tcgcgcgtga
      361 acgagacatt caggtgattt cgttcgacga tttaactcgt gcggatgttg ccaaacgcgt
      421 cttcgggcag ggatgctgat ggccaagcgg gtccgaattt caaaatctgc gccagccgcc
      481 taagccgcca gttcagcagg tgttgaatcc aaaagattgt ccaagtgcgt cagttgaaga
      541 atgcggcgca cgggcggcgt tggattgact acgcggattg aaatgtcgtg atgatcacgt
      601 tgcgcgttgt tgcggagtgc aatcagcgcg ccgatgccgc cgcagtcgag gaaaccggtt
      661 tccgaaaggt caaccacgat gcttttcgcc ccggtcgcca ttgcagagcg tgcctcttct
      721 gcaaaaccgg cggcgttggc aagggccagt tctttaaggt cactgatgca aacagtctct
      781 ccttgatgac gtattttcat ggcagtttca tttttttaag gtcaagcagc aggtcggcct
      841 cttccttgcc gcgcgagaat atcgccgcaa gctgctgtct ccaactgagt gtctccaact
      901 tgggaaatgc gttgcccaac gagcaaagaa tgaacgggtc atccaccgtt gcgactgagg
      961 atgttttcca attcaccagg gtgctgccca tggcgagcga ttccctgagt tcggtgaatc
     1021 ttcccaccaa ggtgtctgct cccacaaacg agccggaaat ctcgcaatcc gcttcgaccc
     1081 atgcccggtt ttccaaaatg gtcatcggcc cgatgattga acgggccccg acagtgacat
     1141 cgtcgccaat ccaagatggt gcgcacagga cggcatccga agcgatatga gcgcgcgcgc
     1201 ccacccagac tccaggctgg gtttcatgca agccaatccg gttgacccga caggtcgaag
     1261 gcatccaggc catttgcgcc gtgaaccaat cggcatagct gttaaacagc ggacgttccg
     1321 gcaggccggg cagatgcagc attgcgatca tgtcgtaagg gtccggcagc cactcggatt
     1381 tttggagggc gggatatttt gtccgagcca gcccggggga caattcacgc ggttcgacaa
     1441 ggacttccgc gcgcaagccc cagcgtgcgc catcaccaac gatgtggcgc accacttcgg
     1501 gccggtcggc agccatgact tggacctgtg tggcgccaag cccggcaaga tgttcaagcc
     1561 aatattcgag cagggttttc ccaagcatcg gcacagcggc cagcgggacg gtttgggaca
     1621 atgcggaaac ttccgcgcgt ggggatgggc agattataca ggctttcact tagcaggctc
     1681 ctttggctcg gacaacagcg ggcaaggtca gcgccaggat cttgacatcc atccagaaat
     1741 cctggctttc gatgtaactg acgtccaact tcacctggcc ggcgaaatca atttcgctgc
     1801 ggccgctgat ctgccaaaag catgtgatgc ccggcggagc cgccaggcgg cgacggtcgg
     1861 cgagcgaata aagcttcact tcgcgcggca atggcgggcg cgggcccacc aatgacatgt
     1921 cgccgatgaa cacgttgtag agctgcggga gttcatcgag ggaaaattta cggagccagc
     1981 ggcccacgcg cgtgatgcgg ggatcgtcct ggatcttgaa cgtgacgccg ttgccatggc
     2041 ggttcttcgc gatgagatct ttcaggcgcg cttcggcgtc catgcacatg gaccgaaact
     2101 tgaacatctt gaattcgcgc ccgaatcgcc ccacgcgtgt ttgggcgaaa atgatcggcc
     2161 cgccatcctc caacttgacc agcaagccaa tcgcgaacag caccgggctg aacaaaagaa
     2221 gcaacagcag gcttacgatg aggtcgaacg aacgtttgac gacaggtccg ctgtgaacgc
     2281 ggcaatgcca gcaaaggcgt ttgaaagcga gttgaatttg aagttcggcg cggccccaag
     2341 ggcttcgtgc caaaccgtaa tgccgcaaca ggccatcgtt gatttcagct ccacaagtat
     2401 cgcgcataat ttgcgaagaa agctagtctc cgccaagcga ttccacaaag ggggtgaaga
     2461 cccgaaatga aattggactc ataccctaac tgaagaaagc ggatgcacta tatggagtgc
     2521 ggaatgcaac aaaagcgccg taaaaaatgg ataattgcgg cagtttttgc catcaaggag
     2581 catcgaatct gcagcttgaa acaggcattc cggaaatcgg ggcccttcta accgtgaaca
     2641 acatggaaat cggtttacgg ctttgcaaag tgggaaaacg cgacgctcgt caaattggtg
     2701 acggcaaaca cccggcgacc atcgggcagt tgggcttcgg cttcgatcca gcgcgagccg
     2761 ccctttagag gcagttggac ggttttgccc caaacaggtt cgtggttctg ccattcccaa
     2821 accactcttg cgctttgcaa atccagaccg ggtgattcga gagtcgccat cagcattcgt
     2881 gaggaggagt tttcagcagg cgaaaatgcg atgcgagcgt cggcggatct ccatggctga
     2941 tttttaagcg gcgtgcgggc cataagccag gcgagatacg caagactgcg gccctggttc
     3001 aagatcacaa attcggtggt gagattgaaa gtgtccgccc agcgatcata aaacgcatac
     3061 ggcgcagttt cgtcgccatc ggatggaaag ctgagcgcgg tcaattcatt ttgatattgt
     3121 gaaatccagc tatagcctgc ctgcaggtta ccgatgtgaa tgccgtccgg cggaagcgcg
     3181 cgccgctggt tttgcgcgta ttgatgaaca atttcctgag gccgtttcca gccgaggccg
     3241 gtc
//
LOCUS       DASZMM010000277         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3127760, whole genome shotgun sequence.
ACCESSION   DASZMM010000277 DASZMM010000000
VERSION     DASZMM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3127760"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VG754_06665"
     CDS             complement(<1..215)
                     /locus_tag="VG754_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWF18932.1"
                     /translation="MTNPLPASPAKGLEGVVAANTRLSDVQGEAGKLIYCGYDIDELA
                     GRVCFEEVVHLLHHNHLPNRKELHEFK"
     gene            439..912
                     /locus_tag="VG754_06670"
     CDS             439..912
                     /locus_tag="VG754_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF18933.1"
                     /translation="MSPVKKMDRVLKILVVEDNPDDVLLLRQAFKRAGGAFILAVVHD
                     GLEAKAYLNGGGKYSDRAAHPFPDLMMLDLNMPSMNGFEVLEWLRNHSDCKRLMVHVM
                     TASPRESDIRRAYDLHANSYMVKPSRVDELVRFVKSLFEWHRYTALPRCSDDRQL"
     gene            1040..2581
                     /locus_tag="VG754_06675"
     CDS             1040..2581
                     /locus_tag="VG754_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWF18934.1"
                     /translation="MNDKSKKQDGPQPAGDSSGRCPSGVEGLDNILNGGLPSNCFYLF
                     QGDPGSGKTTLALQFLLEGMRRGENALYITLSETKEELLKVARSHGWSLDGIPLVELS
                     AIESLLRPEAQTTVFHPSEMELTEVSRMLREEARKAKPKRVVFDSLSEFRLIAETPLR
                     YRRQLLALKQEFAKYQSTVLLLDDKMDSQRFGGDPHVLSLSHGVVEMEQFSPDYGSSR
                     RRLRVMKMRGVKFREGYHDYTIETGGLRIFPRLIAAEHHAPFRREQVSSGIKELDGLF
                     GGGLDRGTTTLIMGPAGTGKSSLALQHATQLAGKGEHCMVFTFDETLGVMLARSKALG
                     MDLEPHIQKGLINVQQVDPAELSPGEFAVRILRAAEKGCKLVVIDSLNGYLNSMPGEN
                     YLTNQLHELCSYLNQQGVVTILVLAQHGLVAAAESPVDLSYLADTVINIRYFEAFGEV
                     RQALAVVKKRSGQHEKTIREFKLEQGKGLRVGEPLKEFQGILSGVPTFHGKAAQMMNE
                     SDAAR"
     gene            2568..>3705
                     /locus_tag="VG754_06680"
     CDS             2568..>3705
                     /locus_tag="VG754_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWF18935.1"
                     /translation="MQPDKPDERVLIVAPVGQDGAVMSSLLKGQNFSTHICESPQECW
                     LRISEGAGALLLTEEVLEFPDFPDLLEHLKNQPPWSELPLILLTRGGESRAARLLEMT
                     ASAAGSVTLLERPMSAATLWRSVQVALNSRRRQYQTRDLIEELRQRQQQLAEQTRLLD
                     LSNDAIIVRDTQERVTYWNKGAEEIYHYSRAEALGQPLKKFLRTEFPEPIEDIHQQLH
                     RKWRWSGELIHTRKDGAQIVVASRWVLDRDAEGKPAFILETNNDITERKVAEAALRRS
                     EERYRLLVSQVKDYAIFSMDVEGRATSWNEGVGRVLGFAQEEFIGAEIASRIFTPEDL
                     QAGVPQRELLTAREQESASNDRWMRKKDGTRFFASGITTALKDDS"
BASE COUNT          892 a          946 c         1086 g          781 t
ORIGIN      
        1 tccttgaact cgtgcaattc ctttcgattc ggcaggtggt tgtgatgcag caggtgcacc
       61 acttcttcaa aacacaccct gcccgcaagt tcatcaatgt cgtagccgca atagatcaat
      121 ttgcctgcct cgccctggac atcgctcaac cgcgtgttgg cggcgacaac gccttcaaga
      181 cctttggcgg gagaagcagg aagagggttt gtcatgagtc agcaaaactt atttttaaca
      241 ttcaaaaatc atcaaattgc cctgaaaatc aaggggacaa tctaggtggc gtgtgcgggt
      301 gcgtcaacgg tttttcaggc aaataagtca gtccggaaat gaaaagacaa acaggcggca
      361 agaaaatcag tttttctgaa aattgacttt aagaaagacc tgaatgaaga tttagaataa
      421 tcggaaccca tggcaggaat gagccctgta aaaaagatgg atcgagttct taaaattttg
      481 gtggtggaag acaatccgga cgatgtgttg ctgttgcggc aagctttcaa gcgagcggga
      541 ggagcgttca ttctggcagt agtccatgat ggcttggagg cgaaggcata tctgaacggc
      601 ggagggaaat atagtgatcg cgcagcccat ccgtttcccg acttgatgat gctggatttg
      661 aacatgccga gcatgaatgg gttcgaagta ttggaatggc tgcgcaatca ttcggattgc
      721 aagcggctga tggttcacgt gatgacggct tcaccgcggg agagcgacat ccgccgcgcc
      781 tatgatctcc acgccaacag ctatatggtg aaaccaagcc gcgtcgacga attggtgaga
      841 tttgtgaaga gcttgtttga gtggcaccgt tataccgcct tgccacggtg ctcagacgac
      901 cgccaactct gaatcaggtt tcacccgaac gattgatccg gaaacgccgc gacatatcag
      961 ggttggcact tgcagtgatt aaaatattcg tcatcgtggc gggaatccgc caaaccaaaa
     1021 tttcagcagc ataatttgca tgaatgataa gtcaaaaaaa caagatgggc cgcaacctgc
     1081 aggtgacagt tctggccgtt gtcccagtgg tgtggaaggg ctggacaaca ttctcaacgg
     1141 cggattgccg agcaattgtt tttacctgtt ccaaggggac cccggttcgg gcaagacaac
     1201 gctggccctg caattcctgc tggaaggcat gcgccgcggc gagaacgccc tctacatcac
     1261 gctctcggag acgaaggaag agttgctgaa agtggcccgc tcgcacggct ggtcgctgga
     1321 tggaattccc ttggtggaat tgtcggccat cgaatcattg ttgcgaccgg aagcgcagac
     1381 taccgtattt catccctcgg aaatggaatt gaccgaagta agccggatgc tgcgggagga
     1441 agcgcgcaag gcgaagccca agcgcgttgt ttttgattcg ctttcagaat tccgcctgat
     1501 cgccgagacg ccgttgcgtt atcgccggca gctcctggca ttgaaacaag agtttgccaa
     1561 gtaccaaagc acggtgttgc tgctggatga caagatggac tcccaacgtt tcggcggtga
     1621 tccgcatgta ttgagccttt cgcatggtgt cgtcgagatg gaacaattct cgcccgatta
     1681 cggttcgtcg cgccgcagac tgcgcgtgat gaagatgcgc ggcgtcaagt ttcgggaggg
     1741 ttatcatgat tatacaatag aaaccggcgg gctgcgtatt ttcccgcgac ttatcgccgc
     1801 cgaacatcac gcgccgttcc gccgcgaaca agtctccagc gggataaagg aactggacgg
     1861 gctgtttgga ggcggattgg accggggaac gaccacgttg atcatgggac cagccggcac
     1921 tggcaaatcg tctcttgcgt tgcaacatgc cacccaactt gcaggcaaag gcgagcattg
     1981 catggtgttc acgttcgatg aaactctcgg cgtgatgctg gcccgctcca aagcattagg
     2041 aatggatttg gagccgcata tccaaaaggg attgatcaat gtgcagcaag tcgatccggc
     2101 cgagctttcc ccaggcgaat tcgcggtccg catccttcgc gcagcggaaa agggctgcaa
     2161 actggttgtg attgacagtc tcaatggtta tctcaattcc atgccgggcg aaaattatct
     2221 caccaatcag cttcacgaac tttgctccta tctcaaccag cagggcgtgg tcacgatttt
     2281 ggtgctggcg cagcatggat tggttgcagc cgctgaatcg ccggtggact tgagttacct
     2341 ggcggacact gtgatcaaca tccgctactt tgaagcgttc ggtgaagtcc ggcaggcctt
     2401 ggccgttgtg aaaaaacgca gcggccagca cgaaaaaacc atccgtgaat tcaagctgga
     2461 acaaggcaag ggcctgcgcg tgggcgagcc attgaaggaa tttcagggca tcctgtccgg
     2521 cgtcccgact tttcacggca aggcggcgca aatgatgaac gaaagcgatg cagcccgata
     2581 aaccagatga gcgggtgctg atcgtcgcgc cggtcgggca ggacggcgcg gtcatgtcat
     2641 ccttgctcaa aggacaaaat ttttcgacgc acatttgtga aagcccgcaa gaatgttggc
     2701 tgcgcatttc agaaggcgcg ggcgccctcc tgttgactga ggaagttttg gagtttccag
     2761 actttcccga tctgctcgaa catctcaaga atcagccgcc ctggtccgag ttgccgctga
     2821 tcctccttac gcgcggtggt gaatcgcgcg cggcaaggtt gctggaaatg acggcatcgg
     2881 cggccgggag cgtcactttg cttgagcggc ccatgagtgc ggcgacattg tggcgctcgg
     2941 tgcaggtcgc ccttaattcc cgacggcgac aataccagac acgtgacctg attgaagagc
     3001 tgcgccagcg gcagcaacaa ctggcggaac agacccggct tctggatttg agcaacgacg
     3061 ccatcatcgt tcgcgacacg caggagcgcg tcacctattg gaacaagggc gcggaagaaa
     3121 tctatcatta ttcgcgagca gaggcattgg gacagcccct gaagaaattt ttgcgcacgg
     3181 aatttcctga accaattgaa gacatccacc aacaactcca ccgcaagtgg cgttggagcg
     3241 gtgaactcat ccacacccgc aaagacggtg cgcaaatcgt ggtcgcgagc cgttgggtgc
     3301 tggatcggga cgccgaggga aagccggcct tcatcctcga aacgaacaac gacatcacgg
     3361 agagaaaagt ggctgaagcg gcgttgcgcc ggagcgagga gcgttaccgg ttgctcgtgt
     3421 cgcaggtgaa ggactacgcc attttcagca tggatgtcga aggccgcgcg accagttgga
     3481 acgaaggcgt gggacgcgtg ctggggttcg cgcaagagga attcatcggc gctgagatcg
     3541 catcccgcat cttcacacca gaagacctgc aggctggcgt gccgcaacgc gaattgctca
     3601 ctgcgcggga gcaggaaagc gccagcaatg accgttggat gaggaaaaag gacggaaccc
     3661 gtttcttcgc ctcgggcatc acgaccgcgc tcaaggatga ttcgg
//
LOCUS       DASZMM010000278         7788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5331383, whole genome shotgun sequence.
ACCESSION   DASZMM010000278 DASZMM010000000
VERSION     DASZMM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5331383"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1561)
                     /locus_tag="VG754_06685"
     CDS             complement(254..1561)
                     /locus_tag="VG754_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF18936.1"
                     /translation="MRKFVSIGLFVLMFSFSASLTLFAQTPPPNDNLTNATLLMGTDV
                     TFIGCLAGSTLEDDADWFFDDYVTFPSTGSIWWSWTAPVSTVLNLEVNAPAPYSSILD
                     GIAIFSETNGTTSPAGLYRPPPGSRLLAAGVDHQYLSIPVTAGSNYLIQLLGTGSNNY
                     TIRMVATNVPLIFKQPLSQTVYSNASAIFYVVSGGLQPRSYQWFFNGTNIPGETAPIL
                     ALTDIDASMTGSYSVLVSNATGSVMSDSATLAISQSNVPPVLAAVGYHSNSLLFSLTG
                     ELGRTYRIQSSTNLIDWSLENSFPVPPGLSSKLLSSNLVYTTTSPLLLMVTNSSSHKY
                     YRAYIYKPDSPESEICINNLRQIVIAKTLWSVDNTDFLGSMGPLVTTPAFSWLAAYFA
                     HPERVFCPEDAATGFQTSYYIDALDRFPSCYVDPAHHRIEGVP"
     gene            complement(1762..2610)
                     /gene="gluQRS"
                     /locus_tag="VG754_06690"
     CDS             complement(1762..2610)
                     /gene="gluQRS"
                     /locus_tag="VG754_06690"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWF18937.1"
                     /translation="MAEKQYRGRIAPSPTGLLHLGHARTFWTAQQRAREHNGVLVLRD
                     EDLDAARCKPEFVSAMYEDLRWFGFEWQEGPDCGGLFAPYSQSERTNFYSAAFEKLLA
                     GNFVYPCACSRKDLQSAPSAPHAADDELIYPGTCRPAGASSKFKVQDLKSGQSHRFFI
                     PNGQTISFTDGHFGPQQFVAGKDFGDFVVWRQDGIPSYQLAAVVDDAAMQITEVVRGA
                     DLLISTARQILIYRALGLTPPAFYHCPLMLDEHGQRLAKRHDALGLRTLRKQGASPED
                     LRQKWS"
     gene            2743..5064
                     /locus_tag="VG754_06695"
     CDS             2743..5064
                     /locus_tag="VG754_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HWF18938.1"
                     /translation="MNARIRNFVAHPLFCFAALCLAFSATAQTNQPIYTDSLQNGWQN
                     WSWATVNIADTSPVHSGTRSISVTCLSNNYQALYLHQTAFDSTPYTNITFWMYVTNSN
                     VPVKVQGTLNGTAQSSYYQVPTLTTNTWTLITVPLSALGVANQPNMDGFWIQNENGSP
                     IPTFFVDDITLNTNVAGPPGTNSTAVITIDAGANRHPISPLIYGVAFATQPQLADLNA
                     PLNRSGGNLETRYNWQLNAHNHDDDYYFESIADSPAVPAADTDSFISGTISGGAKPLI
                     TVPMIGWIPKLGPSRGKLASYSIAKYGPQTSVDPYDTDAGNGISVTNNALITWNDPND
                     ANSPTNVSFWKAYVQHLTNAWGASSNGGVGYYLMDNEHTIWHSTHRDVHPVGTTMQEI
                     LGDIITYAGMVKSVDSNALVLGPEEWGWSGYLYSGYDQQWAGANNNYNPANYPDRSSN
                     GGWDYMPWLLNQLHQYQTTNGARLLDYFTLHCYPQEGSVSGNAIDYNTEILRNQSTRQ
                     FWDSNYVDPSWINSVIMLIPRMSNWVSTYYPGTKIGITEYNWGAEPNMSGATAQADIL
                     GIFGRQSLDLATRWTTPATNTPTYLAIKMYRNYDGNKSTFGDASVLASGPNPDNVSVF
                     AATRTADAALTVMVVNKYLSGTTPVALTLSNFLSTGTAQVWQLNSSNAISRLSDVAVN
                     AGNVNLSVPAQSVTLLVISAKPFNLQSPAARSNGNIGFSLVGEIGQTYIIQSSSNLVS
                     WQSISTNTLTSTQTNFVFSANASRGFYRAMRVP"
     gene            complement(5131..5991)
                     /locus_tag="VG754_06700"
     CDS             complement(5131..5991)
                     /locus_tag="VG754_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF18939.1"
                     /translation="MQKTEQLVLITGAGGLIGNYLLQTAPDFARGQKVIGLTRKHLDL
                     TDFSAVRKLFQGKSPQLVIHCAALSKSPACQADPAVARKLNVEVTALLAELAAEIPFI
                     FFSTDLVFDGKKGDYDESVAVNPLSVYAETKVAAEKIILSNPKHTVIRTSLNGGISPT
                     GDRGFNEEIRRACQEGKMLNLFVDEFRSPIPATVTSRAVWELAASNRPGLYHLAGSER
                     LSRLQIGQLLAARWPQLNPKIQPGSLKDYQGAPRSPDTSLNCAKVQKLLSFPLPSLTS
                     WLAEHRDELF"
     gene            6193..7710
                     /gene="cobA"
                     /locus_tag="VG754_06705"
     CDS             6193..7710
                     /gene="cobA"
                     /locus_tag="VG754_06705"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWF18940.1"
                     /translation="MKSKGTVYLVGAGPGDAGLLTLRGAELLGRAEVVVYDALVNRDL
                     LRMAPKNAEIIYAGKRSADHAIPQEELNNLLVAKAREGKIVVRLKGGDPYIFGRGGEE
                     ASQLAQANIAFEVVPGISSTVAGPNYAGIPITHREHCSSYTVITGHEDPTKDESSIDW
                     AEVAKISGTKVILMGVSRIREIAGKLVEHGLDASTPVAMVRWGTTGRQQSIEGTLATI
                     ADVVEKTQFSAPAVTVIGDVVKLRKTLNWYERRPLFGKRIVVTRTREQASELTRQLAE
                     LSAEVLEIPTIKILPTDRRADLADALLELNSYNWIVFTSPNGVTMFFESFFKAFEDLR
                     DIGGVRIAAVGPASAAKLKELHLKVDVMPAEYLSAKIVKALASFESIENLKILLMRAE
                     VASPDLPRDLEAMGAIVDDVACYKTVPETEDVNGAATTLTEEGADWITFTSSSTVENF
                     HARFDLPALVKKIPKIKIASIGPETTKALTTLGLKPAVEANPHTIEGLVNALVKG"
BASE COUNT         1878 a         2022 c         2177 g         1711 t
ORIGIN      
        1 accgtccttt tgcggagttt cgagccgtga agggcaggat tgtgcaggca gaacagacgc
       61 gcaaggggcg aaaagaaatt catggccagc aaatggcttt ttacgcgggc accggatggt
      121 gacctatgtg cagctcccta gctcacgggc tgattgtctc atcccgattg cgtgttgtcg
      181 agaaacttat ggaaaagcga ccccagttct tcttcaaatc agagacccac gatgaacaat
      241 tctccatgaa gtttcacgga accccttcga ttcgatggtg agccggatca acatagcaag
      301 atgggaaccg gtcgagggca tcgatatagt aggaagtctg gaatcctgtg gcggcgtctt
      361 cggggcaaaa aacacgctca gggtgggcaa agtaggctgc aagccaggaa aaggcggggg
      421 tcgtgacgag gggccccatc gagcccagaa agtcggtgtt atccacgctc cacaatgttt
      481 tagcgatgac gatttggcgc aggttattga tgcagatttc gctctccggg ctgtctggtt
      541 tatagatgta cgcgcgataa tatttgtgtg aggaactgtt ggtcaccatc aacaacaggg
      601 gagaagtggt ggtgtaaacg aggttgctgg ataacagctt ggacgataat ccgggcggaa
      661 cgggaaagct gttttcgagc gaccaatcta tcaaattggt tgaactttga atccggtatg
      721 tccggcccag ttcgcctgtc aggctgaaca aaagagagtt tgaatgatac cccacagccg
      781 caagcaccgg cggaacattc gactggctga tggctaatgt tgcggagtcg ctcatcaccg
      841 agcccgtggc attactcacc agcaccgagt aagagcctgt catgcttgcg tcgatatcgg
      901 tgagtgcaag gatgggcgcg gtttcgccgg gaatgttggt cccgttgaag aaccattgat
      961 agcttcgcgg ttgaaggcca ccggaaacga cataaaaaat ggcgctcgca ttggagtaaa
     1021 cggtctggct taaaggttgt ttgaaaatca aaggtacatt tgtcgccacc attcgaattg
     1081 tataattatt cgagccggtc cccagcagtt gaatgagata attgctgccc gcagtgacgg
     1141 gaatggaaag gtactgatga tcgacgcccg ccgccaaaag ccttgaaccc ggcggtgggc
     1201 gatacagacc cgccggcgaa gtggtgccgt tggtttcgga aaagattgcg atgccgtcaa
     1261 ggatcgagga gtaaggagcg ggagcattca cttccaggtt gaggacagtg gaaacgggag
     1321 cggtccatga ccaccaaatg gagcctgtgc tggggaaggt cacataatcg tcgaaaaacc
     1381 aatcagcatc atcttccagc gtcgaaccgg cgaggcaacc gataaatgtg acatccgttc
     1441 ccatcaggag ggtggcattg gtcagattat cattgggcgg cggtgtttga gcgaaaagtg
     1501 tgagtgatgc ggagaagctg aacatcaaga caaagagccc tatcgaaaca aactttctca
     1561 taattatttt tgaggagatt ttcttcctgc ccgcaaagaa gcagaatatc tgccatagca
     1621 tcgtgatatt gcgtgtgagg gagagagttt gcgcaaaata aacgttgctg atccgaaaca
     1681 ggacagccgt gcccattttt tatcttgtcg aactccgtgc taaatccaaa caatccgttc
     1741 agccaaagac gattccatgt gtcagctcca tttttgacgc aagtcttcag gcgacgcgcc
     1801 ttgctttcgg agggttcgca agcccagcgc gtcgtgccgt ttcgccaggc gttgtccatg
     1861 ttcatccagc atcaacgggc agtgataaaa tgcgggcggg gtaaggccga gggcgcgata
     1921 aatcaaaatt tgccgcgcgg tggaaatcaa taaatccgcg ccgcgcacga cttcggtgat
     1981 ttgcatcgcg gcatcgtcca cgacagccgc gagttgatag gacggaatac catcctgacg
     2041 ccagacgaca aaatcgccga aatcttttcc ggcgacgaac tgctgcgggc caaagtggcc
     2101 gtcagtgaat gaaatggtct ggccgttggg aatgaaaaac cgatgggatt gtcccgactt
     2161 taaatcttga accttaaact ttgaacttgc gccagcaggc cggcatgttc cgggataaat
     2221 gagttcatcg tcggcagcgt gcggcgcgct gggggcggat tgcaggtctt tgcgcgagca
     2281 ggcgcaggga taaacgaaat tgccggcgag aagtttctcg aacgcggcgc tgtaaaaatt
     2341 cgtgcgctcg ctttgggaat atggcgcgaa gagtccaccg caatcgggtc cttcctgcca
     2401 ctcaaaacca aaccagcgca ggtcttcata catcgcggaa acgaactcag gcttgcaacg
     2461 cgccgcgtcc agatcttcat cgcgcagcac aagaacgcca ttgtgctcgc gggcgcgttg
     2521 ttgcgcggtc cagaatgtcc gcgcatggcc gagatgcaaa agtcccgtcg gcgagggagc
     2581 gatgcgtccg cgatattgtt tttcagccat ttgcgccaaa gattgcttgc aaacctacca
     2641 gcgaatcgaa agtgaaaaaa gccccttgtc atcaatcgtg tcatcaattt ttgttgcgca
     2701 agagttttgc ctcccggcgc gggttgtggg aaaattgaat tgatgaacgc ccgaatccgc
     2761 aattttgtcg cgcatccatt gttttgcttc gccgctttgt gcctggcttt ttccgctacc
     2821 gcccagacca atcagcccat ttacaccgat tcattgcaaa atggctggca gaactggagt
     2881 tgggcgacgg tcaatattgc cgacacaagt cccgtccatt ccggcacgag atccatcagt
     2941 gtaacctgtc tgagcaacaa ttatcaggca ttgtatctgc atcaaactgc gttcgattcg
     3001 actccttaca cgaacatcac gttctggatg tatgtgacga acagcaatgt tcctgtgaaa
     3061 gtccagggca cgttgaatgg aactgcccaa agttcctatt atcaggttcc gacactgacg
     3121 accaacactt ggacgctcat cacggtcccg ttgtccgctc tcggagtggc gaaccagccg
     3181 aacatggacg gcttttggat ccaaaacgag aacggcagcc cgatcccaac tttttttgtc
     3241 gatgacatca cgctgaacac aaatgttgcc ggaccaccgg gcaccaacag caccgccgtc
     3301 atcacgattg atgcgggcgc gaatcgtcat cccatcagcc cgttgattta tggggtcgcg
     3361 tttgccacgc aaccgcaatt ggcggacttg aacgcgccgt tgaaccgttc cggcggcaac
     3421 ctggagacgc gttacaactg gcaactcaac gcgcataacc acgacgacga ttattatttc
     3481 gagagcatcg cggattctcc cgcagtcccc gccgccgata ccgacagttt catcagcggc
     3541 acgatttccg gcggcgcaaa gccgttgatc accgttccca tgatcggttg gattccgaag
     3601 ctcggcccaa gccggggcaa gctggccagt tattccatcg cgaagtacgg gccgcaaacc
     3661 agcgttgacc cgtatgatac ggatgcgggc aacggcatca gcgtgacgaa caatgcgctc
     3721 atcacgtgga acgatccgaa cgatgcgaat tctcccacca atgtttcgtt ctggaaagct
     3781 tacgtgcaac acctgactaa cgcgtggggc gcatcgagca acggcggagt tggttattac
     3841 ttgatggata acgaacacac catctggcat tcaacccatc gcgatgttca tccggtcggc
     3901 acgaccatgc aggaaatcct cggcgacatc atcacgtacg ccggcatggt gaaaagcgtg
     3961 gattccaatg cgctcgtgct tggtcccgag gaatggggat ggtcaggtta cctttacagc
     4021 ggctacgacc agcaatgggc tggcgcaaat aataattaca acccggccaa ttatcccgac
     4081 cggagcagca acggcggttg ggattacatg ccatggcttt taaatcagct tcatcaatat
     4141 caaaccacca acggcgcgcg gttgctcgat tatttcacgc tgcattgcta tccgcaggaa
     4201 gggagcgtga gcggcaatgc cattgattac aacacggaaa tccttcgcaa tcaatccacg
     4261 cggcaatttt gggacagcaa ctacgttgac ccaagctgga tcaacagcgt catcatgctc
     4321 atcccgcgca tgagcaactg ggtttcgacg tattatccgg gcacaaaaat cggcatcacc
     4381 gaatacaact ggggcgcgga acccaacatg agcggcgcca cggcgcaggc ggatattctc
     4441 ggtattttcg ggcggcaatc gcttgacctt gccactcgct ggaccactcc ggcgacgaac
     4501 acaccgactt acctcgccat caaaatgtat cgaaattacg acggcaacaa atccaccttc
     4561 ggcgacgcga gtgttttggc cagcggcccc aatccggaca atgtctctgt atttgccgcg
     4621 actcgaactg ctgacgcggc gttgacggtg atggtcgtga acaaatattt gagcggaaca
     4681 acgcccgtag cattgacact gagcaatttt ttgagcacag ggaccgcgca agtgtggcaa
     4741 ctcaattcga gcaatgcgat ttcccggttg agcgacgttg ccgtgaatgc gggaaatgtg
     4801 aatctttccg tgccggcgca aagcgtcact ttgctggtga tttcggccaa gccgttcaat
     4861 ttgcaatcac ctgccgcgcg ctcgaatggg aacatcggtt tttcgctggt tggcgagatt
     4921 gggcagacct acatcatcca atcctccagc aatcttgtga gctggcaatc gatttcgacg
     4981 aacactctga ccagcacgca aaccaatttt gttttttctg cgaatgcttc gcgtggattc
     5041 tatcgagcca tgagagtgcc gtgatctgtt gcgctaatta ttttgcggga gcgacgtgag
     5101 gaggctctga ctttaaattg tgccactggc ttaaaatagt tcatcgcgat gttccgccag
     5161 ccaggaagtg agagagggca ggggaaagct caacaatttt tggactttgg cgcaattgag
     5221 tgaagtgtcg ggcgagcgag gcgcgccttg ataatctttc aacgaacccg gctggatttt
     5281 tggattgagt tgcggccagc gcgcggcgag aagctgtccg atttgcaatc gcgacaatcg
     5341 ttcgcttccc gccagatgat ataatcccgg tcgattgctt gcggccagtt cccaaacagc
     5401 gcgtgacgtc acggtcgccg gaatgggaga acgaaattcg tccacaaaaa ggttcaacat
     5461 tttgccttcc tggcatgcgc gccggatttc ctcgttgaat ccgcgatcgc ctgtgggcga
     5521 aatgccgcca ttgagcgaag tgcggatgac cgtatgtttg ggattcgaca aaatgatttt
     5581 ttcagcagcc actttggttt ccgcataaac actcaacgga ttaaccgcga cggattcatc
     5641 gtaatcgcct tttttgccgt cgaataccaa atcagttgag aaaaaaatga aagggatttc
     5701 agcggcaagt tccgcaagca acgcggtgac ttccacattc aacttgcggg ccaccgcggg
     5761 gtcggcttga caggccgggc ttttgctcaa tgccgcgcaa tgaatcacca gttgcggcga
     5821 tttcccctga aataatttgc gcactgcgga aaaatcagtg agatcgagat gcttgcgcgt
     5881 gagcccgatc accttctggc cgcgcgcaaa atcgggagcg gtttgcagca aataattccc
     5941 gatgagtccg cccgcgccgg tgatcaagac gagctgttcg gttttttgca cattggtgtt
     6001 tacaacgcgc ttggttgtga ggcaatgccg aatggcagtc gtagagaagc agggaactga
     6061 ttgaatgaag gaactcatga actgacccag gaagtcgaaa atcttttcct cagttccttg
     6121 tctctccccg ccttcccggt tcaatttcca cgttattgca cgttttcgtc cgcgcgttta
     6181 gaatgggaaa taatgaaatc caaagggaca gtttaccttg tgggcgcggg gccgggtgat
     6241 gccggcttgc tcactttgcg cggggcggaa ttgctcgggc gcgccgaggt cgttgtttat
     6301 gacgcgttgg tgaatcgcga tttgctgcgc atggcgccga agaacgccga aatcatttac
     6361 gcgggcaaac gctccgccga ccacgccatt ccccaggaag aactgaacaa cctgctcgtc
     6421 gcaaaggcgc gcgaaggaaa aattgtcgtg cgcctcaagg gcggcgatcc ttacatattt
     6481 ggccgcggcg gcgaggaagc cagccaactt gcgcaagcga acatcgcgtt cgaagtggtg
     6541 ccgggaattt cttcgaccgt tgctgggccg aattacgcgg gcattcccat cacgcatcgc
     6601 gagcattgtt cgagttacac ggtcatcacc gggcacgaag atccgacgaa agacgagtcg
     6661 agcattgatt gggcggaggt tgcaaaaatt tccgggacga aagtcatctt gatgggtgtt
     6721 tcgcgcatcc gtgaaatcgc cggaaaactt gtcgagcacg gtcttgacgc cagcacgccg
     6781 gttgcgatgg tgcgttgggg aacaaccggc cggcagcaat ccatcgaagg cacgctcgcg
     6841 accatcgctg atgtcgttga aaaaacccaa ttcagcgctc cggcggtgac ggtgattggc
     6901 gatgtggtga aattgcgcaa gaccttgaac tggtatgagc ggcggccttt gttcggcaaa
     6961 cgcatcgtgg tgacgcgcac acgcgaacag gccagcgaat tgacgcgcca gcttgccgag
     7021 ttgagcgctg aagtgcttga aattcccacc atcaaaattt tgcccacgga ccgacgggcc
     7081 gatctggccg acgcgctgct ggaattgaat tcctacaatt ggatcgtctt caccagcccg
     7141 aacggcgtga cgatgttttt cgaatcgttt ttcaaggcgt ttgaggacct gcgcgacatc
     7201 ggcggcgtgc gcatcgccgc cgtcggcccc gcgagcgccg cgaaactcaa ggaactgcat
     7261 ctcaaagtcg atgtgatgcc cgcggaatat ctcagcgcga aaatcgtcaa ggcgctggcg
     7321 agtttcgaga gcatcgaaaa cctgaaaatt cttttgatgc gcgccgaggt tgccagcccg
     7381 gatttgccgc gcgacctgga agcgatgggc gcgatcgtgg acgatgtggc ctgctacaaa
     7441 actgtgccgg aaaccgagga tgtcaacggc gcggcgacaa cgttgacgga agaaggcgcg
     7501 gattggatca cgttcacgag cagttcaacg gttgaaaatt ttcatgcgcg atttgatctg
     7561 cccgccttgg tcaaaaaaat tccgaagatc aaaatcgctt ccatcggccc tgaaacgacg
     7621 aaagcgctca caacccttgg tttgaaacca gcagtcgaag cgaacccgca tacgattgag
     7681 gggttggtaa acgcgcttgt gaaggggtga ggctcgtcag attggaaatt tcaaatttca
     7741 aatccaaaca gagcctgcgc gtcggcgcgc aaaaaaagtt tatgaacg
//
LOCUS       DASZMM010000279         6733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4781756, whole genome shotgun sequence.
ACCESSION   DASZMM010000279 DASZMM010000000
VERSION     DASZMM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781756"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            651..1649
                     /gene="bamD"
                     /locus_tag="VG754_06710"
     CDS             651..1649
                     /gene="bamD"
                     /locus_tag="VG754_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWF18941.1"
                     /translation="MKQRRLILCLVLLCTLGLPFRSPAPLIYTPGEGWTYEPAGGEGQ
                     WRKVRAKDQLDVAQKAFDAKDYGLAKKAAERVVTGWPFSDYAPQGQYLLARCSEQLGQ
                     DEKAFREYQKLLEKYPKIPNYEDVLQRQFLICNKFLNGERFKLWGYVPTFPSMDKTVT
                     MYEKVIKNGPYSDVAAQSQMNIGTAREKQSRYFNDNEPYEQAAKAYELAADRYHDHPK
                     FAADALYKAGLAYQKQASTAEYDQSTAGQAIATFQEFMTLYPNDPRVLNGEKIIASLK
                     TEQARGNFETAKFYEFNHRWKSALIYYNEVVIKDPNSPYAETAKKRITALKKLTEK"
     gene            1649..2167
                     /locus_tag="VG754_06715"
     CDS             1649..2167
                     /locus_tag="VG754_06715"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HWF18942.1"
                     /translation="MKRSPSLWRLCALALLFTAGCAGYHLGPTAGQVAGARSIQIAPF
                     VNRTLEPRLSQYVMMSLRKNLMQDGTYRLDTHDEGDVILTGEIKTYQRNGLSFQPTDV
                     LTVLDYEITMTAQITARERNTGKVIFDKAVIGKTTLRAGPDLTSAERQAIPLLTDDLA
                     KKTTALLVEGNW"
     gene            2336..2719
                     /locus_tag="VG754_06720"
     CDS             2336..2719
                     /locus_tag="VG754_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18943.1"
                     /translation="MIKSIGKLTLATILAALVVGLPIGASAQDKSTSTNAAPATPPKP
                     RAIPFHGKLGAMDKVSKTITLDDKNKRTFDVTSETKITKGKDKKPATLDDGVVGQEVS
                     GSYVKGADGKMALRSLRFDPKGESK"
     gene            complement(2777..3751)
                     /locus_tag="VG754_06725"
     CDS             complement(2777..3751)
                     /locus_tag="VG754_06725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWF18944.1"
                     /translation="MITCGGCGAKVFIPANLQPLTTTPCPKCNFAIMMPMQMRQFELR
                     AFIASGGMGTVYRAYDTVLEREVALKVMKPELASDPQALESFYREARACASLNHTNII
                     HIYTFDEYERQKYLVMELADQGSLDSRIELYQRLCELDVLDIGIKVASALDLALKHGF
                     LHRDIKPGNILFNVDNEPKLVDFGLARKAEAEHNPEEGAWGTPYYVAPEKIRREPETF
                     LSDMYSLGGTLYHAITGHVPFEAPTVEELVLAHVNVPLTPPNQVVPEISEAGSDSLVR
                     AMAKSPAERFQSYDEFIMAMHAARANLLFQQSQGKGPSPVKGKSWWKR"
     gene            4116..4454
                     /locus_tag="VG754_06730"
     CDS             4116..4454
                     /locus_tag="VG754_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18945.1"
                     /translation="MKNKTYDKIRTRFAPEVRFDVPTAPFRATETTALDELKTRLLKQ
                     LLEQAAEPGQNVLLRRAANDAAAIAWLTQFPLLLFPTLLEEKARAAFAQHKRQAGIRR
                     RSQNLLQEAA"
     gene            4765..6201
                     /locus_tag="VG754_06735"
     CDS             4765..6201
                     /locus_tag="VG754_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HWF18946.1"
                     /translation="MSANPPVANPWMILPFCILMALIALGPLGFPKWWHRHYPKATYA
                     LALITMVYYFFGLHAGQRIGHVAHEYISFIALIGSLFVVAGGIHINVKGEATPFVNMV
                     FLLIGAILANLLGTTGASMLLIRPWLRMNKYRVTAHHVVFFIFIVSNIGGCLTPIGDP
                     PLFLGYLAGVPFWWVAKHCFPMWLVGMAVLLAMFFVVDYRNYLRAPKAVRSELTEHDQ
                     WRFEGLGNVGFLGVILAAVFINQPPYLREALMVGAAIGSYFTTRADVHAANHFDFHPI
                     KEVAVLFIGIFATMLPALDWLEANAKGADHINISWVFWGCGSLSSALDNAPTYLCFLK
                     ALTVHFASPDIVGGVNHLIQTGGVDITQVSGPHAEQIRQTFLILQKYYAVELASGKIG
                     SDQIAIASLLGNAEGNQSLAAIAMGAVFFGANTYIGNGPNFMVKSIAEHQKVRTPGFL
                     GYLWHFTLPFMLPMLLIVWWLFFRHQTL"
     gene            6339..>6733
                     /locus_tag="VG754_06740"
     CDS             6339..>6733
                     /locus_tag="VG754_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HWF18947.1"
                     /translation="MRTFYNILFTIFFVLASPRYILKMRRRGNWRRGFKQRFGHYDNK
                     IKQSLTNRHVLWMHAVSVGEMNVCMQLIRALEPRLPNFKIVVSTTTTTAMGELQKRLP
                     PSIGKIYYPFDHSSSVARALNTIHPNAIIL"
BASE COUNT         1631 a         1743 c         1762 g         1597 t
ORIGIN      
        1 cgcaaactgg aagaaacatg ctaaagcatg gacaccaaac agccttcgaa gcgccgagcg
       61 tttcagctaa tccgattgtt cggtgtccac gctttagcgt gcatcggcac aaccgcaggg
      121 aacatgctaa agcatggaca ctgaacgacc cttatcgggg agttcaaccg ttttcttttc
      181 tttgcgccct cgtgtctttg cgcctttgcg ttaaatccac gaattttccg ccatcgtcgc
      241 ggagaaattt ccgcttaatt ccgcttgttt ccaccccgtt ccgcttgatt ccgcataaaa
      301 aaaataattt tttcggccag gccatcaacg caaaggcgca gaggcgcaag gaggataagc
      361 gaggctctgg gagtcaagcc ttggaaaacc atgcaaaatg gaacatctca ttctccatga
      421 acgcattaca ccatttttgt ccgaaatcga gtcaaggcaa gtcgtgccat ttttctgcgc
      481 ggtcaaggct tacgtttttt gacccatcac tccatgactc caacactcca ttatcccatg
      541 agtccatcag tccaagactc cattccgaac gccacttcaa ttgccatcac ctgaataagt
      601 ttcccggcgg cattcttgac ttgaatgttt gcgctggcta cggttgcccc atgaaacaac
      661 ggcgtttaat tctttgcctg gtgttgctct gcacgctggg tctgccattt cgttcgcccg
      721 cgccgttgat ttacacgccc ggcgaaggct ggacttacga acccgccggc ggcgaagggc
      781 aatggcgcaa ggtgcgcgcc aaggaccaat tggatgtcgc gcagaaagca ttcgacgcga
      841 aggattacgg tcttgcgaag aaggccgctg aacgcgtggt gacaggctgg cccttttcag
      901 attacgcgcc gcaggggcaa tatttgctgg cgcgttgttc tgagcaactc ggacaggatg
      961 aaaaggcatt cagggaatat caaaaactcc tggaaaaata tcccaagatc ccgaattacg
     1021 aggatgtttt gcagcggcaa ttcttgatct gcaacaaatt cctgaatggc gagcgcttca
     1081 aattgtgggg ttacgtcccg acatttcctt cgatggacaa gacggtgacg atgtacgaaa
     1141 aagtgattaa gaatgggcct tacagcgatg tcgcggcgca atcgcaaatg aacatcggca
     1201 cggcgcgtga aaaacaatcg cggtatttca atgacaacga gccctacgag caggcggcca
     1261 aggcttatga actcgcggcg gaccgttatc atgaccatcc caaatttgcc gctgacgcgc
     1321 tttacaaggc aggcttggct tatcagaaac aggcatcaac ggcggaatac gaccaaagca
     1381 ccgccggcca ggcgattgcc acgttccagg aatttatgac gttgtatccc aacgacccgc
     1441 gtgtcttgaa cggggagaaa atcattgcgt cgctaaagac ggaacaggcg cgaggcaatt
     1501 ttgaaacggc gaagttctac gaattcaacc atcgctggaa aagcgcgctc atttattaca
     1561 atgaggttgt gatcaaggat cccaactcgc cttacgccga gacggcaaag aagcgcatca
     1621 cggcgcttaa aaaactcacg gaaaaataat gaagcgcagt ccgagtcttt ggcgcttgtg
     1681 tgcgctggca ttgttattca cggccggttg cgccggttat catctcgggc cgacggcggg
     1741 acaagtcgcc ggggcgcgtt caatccagat cgctcctttc gtcaatcgca cgttggaacc
     1801 gcggctgagt caatatgtga tgatgtcgct tcggaagaat ctcatgcagg acggcactta
     1861 tcgcttggac actcacgatg agggcgacgt cattttgacc ggcgaaatca agacgtatca
     1921 aaggaacggg ctgagtttcc aaccgacgga tgtgctgacc gtcttggatt atgaaatcac
     1981 gatgaccgcg caaatcaccg cgcgggaacg caacaccgga aaggtcattt tcgacaaggc
     2041 cgtgatcggg aaaacgacat tgcgcgccgg gccggatttg acaagcgccg agcgacaggc
     2101 aattccattg ttgacggatg acttggccaa gaaaacgact gcattattgg tggaaggaaa
     2161 ttggtaaatg tcgaaattta aaaaattaaa tcgggttttg atgcgtgaag ttcacgcaaa
     2221 aggagtggat ttgcagcgca atcaggataa atattcgata aaaaatgact tgcggcaggt
     2281 cgtgatttta taacaatagc gcccgtaatc agagattata accctttaca aaactatgat
     2341 taaatccatt ggcaagttga cactcgcaac aatattggcc gctctcgtgg ttggtttgcc
     2401 catcggcgct tcggcgcagg acaaatccac ttccacaaac gcagcgcccg ccactccgcc
     2461 gaagccccgt gcgattcctt tccacggcaa actcggtgcg atggacaagg tgagcaagac
     2521 catcacgctc gatgacaaaa acaagcgcac gttcgatgtc acttcagaaa ccaaaatcac
     2581 gaaaggcaag gacaagaagc cggcaacttt ggacgacggt gttgttggcc aggaagtgtc
     2641 cggttcttat gtgaaaggcg cggatggcaa aatggctttg aggtcgctcc ggtttgaccc
     2701 gaaaggcgaa tcgaagtaag gcaaaaaaat tccttcaatc aaggcaggcc gttggcctgc
     2761 cttttttatt tgcggctcaa cgtttccacc agctctttcc tttgacggga ctcggccctt
     2821 tgccctggct ttgttgaaac agcaagttcg cgcgcgcggc atgcatggcc atgatgaact
     2881 cgtcgtaact ttgaaatcgc tcggctggac ttttggccat ggcgcgcacc aaggaatcgc
     2941 tgcccgcctc gctgatttca ggaacgactt gattcggcgg agtgagtggt acattcacgt
     3001 gcgccaacac caattcttca acagtgggcg cttcgaaagg cacatggccg gtaatggcgt
     3061 ggtaaagcgt tccgccaagg ctgtacatgt cggacaggaa tgtttccggc tcgcgcctga
     3121 tcttttccgg cgcgacgtaa tagggcgttc cccacgcgcc ttcctcggga ttgtgctcgg
     3181 cttcggcttt gcgggccaag ccgaaatcca cgagcttggg ttcgttgtcc acattgaaga
     3241 gaatgttgcc gggcttgatg tcgcgatgga gaaatccatg tttcaaggcg aggtcgagcg
     3301 cggaggccac cttgatgccg atgtcgagca catccaattc gcaaaggcgc tgatacaatt
     3361 cgatgcggct gtcgagactg ccttggtcgg ccaattccat gacgagatat ttctgccgtt
     3421 catactcatc gaaagtatag atgtggatga tgttcgtgtg gttgagagag gcgcaagcgc
     3481 gggcttcgcg gtaaaaactt tccaatgctt gcgggtcgct ggcgagctcc ggcttcatga
     3541 ccttcaaagc cacttcgcgt tcgagcacgg tatcgtatgc gcgataaacg gtgcccatgc
     3601 cgccggatgc aatgaatgca cgcagttcaa attgccgcat ctgcatgggc atcatgatgg
     3661 cgaaattgca cttgggacac ggagtggtgg tgagcggctg caagtttgcc gggataaaga
     3721 ccttggcgcc gcatcccccg catgtgatca tgcgcaattg cttacgatcg tcctcttgtc
     3781 cagttgtcac ggcctatagc atagcatttt gcgttcccac cacaaggcgc attcttccgg
     3841 tttgacaaga atgacgcaat gtcgtataaa caagcgtcaa tagtgacaaa gtgaaatgaa
     3901 caccacgttt aaaaacaatc tgtgcgccat gagttgcggt cgccaaatga ccatttggca
     3961 tcgcgtctcc tggcttgtcg gcaacgtggg aggaactttc taaaagaacc aatatagaat
     4021 tttcaaaaga cccgcgattg ccaagtcaat cgcgggtttt ttatttcgcc gaagacgccg
     4081 aaatcagcgg caaacccaaa ccactaagca gaataatgaa aaacaagaca tacgataaaa
     4141 tccggacgcg gttcgcgccc gaagtgcggt tcgatgtgcc cacggcaccg ttccgcgcga
     4201 cggaaacgac tgcgctcgat gaactcaaaa cgcggctgct caagcagctt ttggaacagg
     4261 cggccgagcc cggccaaaac gtgctcttga gacgcgctgc aaacgacgcg gcggccattg
     4321 cgtggctcac gcaatttccg ttgctgttgt ttccaacatt gcttgaggaa aaagcgcgcg
     4381 cggcattcgc gcagcacaag cgccaggcgg ggatccgtcg gcgcagccag aacctgttgc
     4441 aggaggccgc atgaaaacgg caaccattac aacgggtgcg actcacggag gggaactgtt
     4501 tacaccgacg atgcatcgcc tgtgcaaacg gttcctcacc gtgatgtcct gtcccgaatg
     4561 caccaacggt tccgcaaaga agattggatg acggcaaaca tacgttgcac caatcgcgcg
     4621 cgagcgtgag agcgcaatga gttgccctcg tccaaccttt gtggacaaat ccggtgagga
     4681 gccgttacgc tttcccgaag tcgcgggaat gcaaacagga ttctcattga ccctattttg
     4741 tgccaaacct tagcttctgc gccaatgagc gcaaatccgc ccgtcgccaa tccatggatg
     4801 atactgccgt tttgcatcct catggcattg attgcactcg ggccgttggg ctttccgaaa
     4861 tggtggcacc gacattatcc caaagcgacg tatgcgctcg ccctgatcac gatggtttat
     4921 tatttcttcg gattgcacgc ggggcaaagg atcggccacg tcgcgcatga atacatcagt
     4981 ttcattgcat tgatcggttc attgtttgta gtcgccggcg gcatccacat caacgtaaaa
     5041 ggcgaagcga ctccgttcgt taatatggtt tttctcttga ttggcgcgat tctggccaat
     5101 ctcctaggca ctactggcgc gtccatgctg ctcattcgcc cgtggttgcg aatgaataaa
     5161 taccgtgtca cggcgcatca tgtggtgttc tttattttca tagtttcgaa tataggcggt
     5221 tgcctcacac cgattggcga tccacctttg ttcctgggct atctggcagg tgttcctttt
     5281 tggtgggtgg cgaaacattg ctttccgatg tggcttgttg gaatggcagt tttgctcgca
     5341 atgttttttg tggtggatta ccggaattat ttgcgcgcgc caaaggctgt tcgcagtgag
     5401 ttgacagagc atgatcaatg gcggtttgaa ggcttgggca acgttggttt tttgggagtc
     5461 attttggctg ccgttttcat caaccaacca ccttatttaa gggaagcgct gatggtgggc
     5521 gcggcaatcg gctcctattt cacaacgcgc gctgacgtgc atgccgcgaa tcatttcgac
     5581 ttccatccca tcaaagaggt agccgtattg ttcataggaa tctttgccac gatgttgcct
     5641 gcattggatt ggttggaggc aaatgcgaag ggggctgatc atatcaatat ttcatgggtg
     5701 ttctggggtt gcggctctct ttcaagcgcg ctggacaatg cgcccaccta tctttgcttt
     5761 ctcaaggcgc tcaccgttca ttttgcaagt ccggatattg tcggcggggt caaccacctt
     5821 atccagaccg gcggcgtgga cattacccaa gtctccggtc cgcatgcgga acaaatccgc
     5881 cagacctttc tcatacttca gaaatattat gcagtggaac tggcctccgg aaagattgga
     5941 agcgaccaaa tcgcgattgc gagcctgttg ggaaatgccg aaggaaacca aagcttggcc
     6001 gccattgcca tgggagcggt tttttttggg gcgaacacat acatcggcaa tggtccgaat
     6061 ttcatggtca aatccatcgc cgaacatcag aaagtccgca cgcccggttt tttgggttat
     6121 ctatggcatt ttaccttgcc attcatgctt ccgatgctgc tcatcgtttg gtggttgttc
     6181 tttcggcatc aaactctatg atgtgttacc cgctcggttg aatcttgaat ttgcaaaaca
     6241 ggggggttgg cgtaagaaat gctaaaaaca aattattcca aaactagaaa tctggttatg
     6301 ctccatcaca ggtggaatgg ctgttagaga attcgcgaat gcgcactttc tataacattc
     6361 tgttcacaat cttctttgtc ctcgcttcgc cgcgttacat tctcaaaatg cggcggcgcg
     6421 gaaactggcg gcgcggtttc aaacagcgct tcggccatta tgacaacaaa atcaaacaat
     6481 ccctgaccaa tcggcacgtg ctctggatgc atgcagtcag cgtcggcgag atgaatgtct
     6541 gcatgcaatt aatccgggcg ctcgaaccgc gcctgccaaa cttcaaaatc gtggtatcca
     6601 ccaccaccac gacagcgatg ggcgaattgc aaaaacggct gcctccaagc attggaaaga
     6661 tatactatcc attcgaccac agcagttccg ttgctcgcgc tctcaacaca atccatccca
     6721 acgccatcat tct
//
LOCUS       DASZMM010000280        11948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4781799, whole genome shotgun sequence.
ACCESSION   DASZMM010000280 DASZMM010000000
VERSION     DASZMM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781799"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VG754_06745"
     CDS             <1..547
                     /locus_tag="VG754_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWF18948.1"
                     /translation="MRPDIGSLLILGAGPIGLGVLMMAKIILGNHVKVYLTDIIPYRL
                     KLAEQLGGLPINVAKGSIKDGLKSHGADAADMAVDTSGKQVAREEGHRALAQRGSLIC
                     VGHGEGISLKVSPDIIAPERSVVGSEYFRFDELPANLRRLTQHRAYLNQIITHRFGVD
                     EIQKSFELFFGGETGKVIIEQ"
     gene            544..1584
                     /locus_tag="VG754_06750"
     CDS             544..1584
                     /locus_tag="VG754_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF18949.1"
                     /translation="MSSKIKAVLIGAGGWGLQHARILSQRSDVEFCAIAGRTLEKTKT
                     RSGQFGVRAYTNITEMIKQEKPDLAFLSLPNDGHFQPTMEVIRAGVPLFVEKPLVFDL
                     KEADKLVTEAAKRKLFFGINFNHHYARPVALAHHAIQQGRLGELTFATWRFGGEGGTS
                     HPFSNLLETQCHAFDMLEFLCGPIDSVMAQMTDKTKKGFYRTMVLALHFKNGAVGSLV
                     GSYDSSYAYPDTHRVEINGTKGRILIEDTVRRYTFNAAGSETAEVWQSGYFNDRDRDF
                     YLTFDRHMDHVMDAFKAGKQPPVHARAGRRALQLAFASIKSFESGKKVSVPTDSFEMA
                     AAPAILPPPPNP"
     gene            1608..3158
                     /locus_tag="VG754_06755"
     CDS             1608..3158
                     /locus_tag="VG754_06755"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWF18950.1"
                     /translation="MSAEATNPAVSFRGIGKRFPGTVALQNVSFDVATGSCHAIMGEN
                     GAGKSTLGKILAGIHQPSEGCIMVEGVERRFHSPLDAQRAGVAIVHQELLFCPNLSVA
                     ENICLSHLPSRMGRLDWPKLYQRAQTFLDQVGAECGAHEELGRLSTGQTQLIQIAAAM
                     ATGAKILVMDEPTSSLSAMETQRLEEIIVRLRQNGATIIYVSHRMDEIFRLCDAVTVM
                     RDGQHVATMPLAQTNEEELVKLMIGRSWQKMFPEHINQAAGPERLRLENFSSLGKFQH
                     INFSIRSGEVLGVAGLVGSGRSEVALGIFGLDPKTSGRVLVDGKESCPRSPRDAMKLG
                     IGLVSEDRKGQGLVLGMECGENITLSSLDRISRCGVIRQGNERGIIAQFFEKLRVSAA
                     SPQVLAQTLSGGNQQKLVLAKWLARESRILILDEPTRGVDVGAKAEIHKLIDELAAAG
                     CAILMISSELPEVLNLSTRVIVMRNGRLAGELNRAEATAGRVLKLMTGCGEEKELRPQ
                     ILSDLAVT"
     gene            3161..4294
                     /locus_tag="VG754_06760"
     CDS             3161..4294
                     /locus_tag="VG754_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF18951.1"
                     /translation="MPSEQPIAAGAEPIARTGRVTSFKRSRFKETGLVGVLVALGLFL
                     SIFGGSLQVTNRDTGNVIKVNKFMRADNLDKLAKNTSFFAIMAVGATFVIITGGIDLS
                     VGAVYCLAAVCGAMFLTRFGPHGIGAGTSPFLVVPGAVLVCLGTGALSGFINGIGVVG
                     LRVHPFVITLGTMAIYRGIAFVMTKAQAFTDFPVQFTDGFIRREITFQGASLYPVPLA
                     IMIGVTAFAWWFLKHSVTGRQIFAVGGNEQASLFSGLPVNWVKVKVYTFSGLAAGIAA
                     MIMLGYYGSASSDAGKGYELDVIAAAVVGGASLIGGRGTALGALLGALIIQVINNGII
                     ILNIDQNYSQIIIGAVIVLTVLLDRINNSMQEKRMTRAAQAAK"
     gene            4337..5350
                     /locus_tag="VG754_06765"
     CDS             4337..5350
                     /locus_tag="VG754_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWF18952.1"
                     /translation="MNITQIITRTALTLSAILIVAGCDQKKSDVTPSAASPGSKKTEY
                     TFAMVAKSQSNPVFQAARVGAEDAAADLSKKLGVKINVLWRTPNEDDAQKQVENIEQL
                     VLQGVDGISVSCSEAETLTPAINKAVESGVAVMCFDSDAPASKRFCYHGTDDIECGHI
                     IMRELATVLGPGKHVVAIPGGNQNARNLQNRIKGAVDEATNYPNISVRGIYYSKEVPQ
                     DAAAKIEEVQTANPDIDGWAMIGSWSLFTDALLKWEPGKVKIVAMDALPAELPYLTKG
                     VVQKLYAQQTYRWGVRSVELLADKVILGKNPANVMDYSELPPVTKDDVQQYEQNWKKW
                     LRQ"
     gene            5459..8782
                     /locus_tag="VG754_06770"
     CDS             5459..8782
                     /locus_tag="VG754_06770"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HWF18953.1"
                     /translation="MANIYVNTSQTLHTNSAYLTGACLEDVNHEIYGGLYSQMVFGES
                     FQEPASPRPLTGFTAYGGSWLPTNGVLQAAAGSGPKLIYNGFNQTNGDVSVQLWFSSN
                     AGGNAGLIVQVSQPGVGADVFNGYEISLAPAGYLVLGRHVQNFTSLSEASCSVPIGQW
                     ITLEVQYTNATFNILVNSNSILQYTDTQQPLTSGQAGLRTWQQSVQFQNFSINTNINI
                     PFQLATNGLAEGVSGMWNPVSTGTVTGQFAMETTDPFVGTQSQRITFTSGTGVVGIAN
                     QGLNRWGMPFVGGSSYTGTLDARADAPTTIWVALESADGSSVYAEQSLSVSSNNWQQL
                     NFNLTPSASDGNGRFTIKLKQPGSMVVGYAFLQPGPWGCLQGLPVRKDVVQGLINQGV
                     TVLRYGGSMVNASGYRWKNMIGSRDRRPPYTGTWYPYSSDGWGIPDFLNFCEAAGFLG
                     VPDFNINETPQDMADFMQYVNGSTNTVWGAQRLADGHPQPYNLKYIELGNEEAVNSTY
                     YQKFQALAQAIWAADTNVILVVGDFSYHQVITNPFSFSGADSGITTLAAQQQILQLAQ
                     TNSRAVWFDVHVWTDGPTPDSSLAGMFSYDTALGQIANGANYKVVVFELNANNHSQRR
                     ALANALAINAIERDGRLPITTSANCLQPDGQNNNGWDQGLLFLNQSNVWLQPPGYVTQ
                     MYATNFQPLEIQSSVTDPNNDLDVSAECSQDGSKLVLKVVNLNSSPESATINISGFAP
                     TNPVAAVQVLASLQSSVNTAQAPFGFVPANISWQHNFNGSNANYTFAANSVTTITFQG
                     QVIVPPPTPPVVLTHRWSFNEPANSAQFVDSVLGITNGIIHGGARIDGNGNLVLAGLN
                     QKTNYAELPPYLLTSTNYTAVTFEFWVAFGTNNTWGRLIDCGDTNPNTGNGRYCLDFT
                     PHSGYGPNGINFEVSGTDPGNSSAQNIAVPPVLDNQGKIHLVLIWDSIARYMAVYTNG
                     VLMGINSSVTLPISAIVNAHSYLGKSSYTGDYCGVATIDEFRMYSGAMGMAQIADDYA
                     SGPNSLPVPKLSIATAGQNLAFTWPDYITGYSLQTSAKLGAGASWVPAAVSSYFLTNG
                     TFLIKVPMTSQQAFYRIVK"
     gene            8811..11294
                     /locus_tag="VG754_06775"
     CDS             8811..11294
                     /locus_tag="VG754_06775"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HWF18954.1"
                     /translation="MINQTGLIKAAAILAGILSVLATVGQTKGATVTVNADQVLHTNS
                     PYLTGACIEDVNHEIYGGIDSQMIFGESFAEPAAQLPLKGFKAYEGRWTLADDGSVQG
                     VGSNGSKIVWDGPALSVGEVSVDVKLTEATGGNGGLILKVSDAGIGADAFTGYEVSLE
                     RPGQLVVGRHRQNWEPIRNVPCDVPVNEWIKLTVRATAKSFEVFVNGKSMLQFEDTEH
                     PIETGTVGLRIWQHKVSFRNLSVATGASQQKVAFDYDAGNNPSDSVSGIWRPIRQGAV
                     KGSFSLESQGAFSGNQSQQITFAGGSGAIGMENQGLNRRGMNFVKGKNYEGYLWARAK
                     SPTEIFVTLENTNGSVVYAEKSMKLKAGDWQRLDFELTPNAVDQAGRFAIKLKRAGSV
                     TVGYAFLQPGSWGRFKNLPVRKDVAEGLINQGVTILRYGGSMVNNSEYRWKKMTGPRS
                     QRPPYEGHWYPYSSDGWGIFDFLNLCEAARIVGIPDLDINESPKDMADFMDYLNGTPD
                     SEWGKKRVADGHPAPYRLKYIELGNEERVDDNYFEKFKAVAEVIWAKDPAIVLILGDF
                     SYHKVITTPFNFTGADSGITTLAGQQRILQLAKQHDREVWFDLHVWTDGPRPDSSLES
                     MFSYIDAMDKIADGAKHKVLVFELNADNHSQRRALANALAINAIQRDGRLPITCSANC
                     LQPDGQNDNGWDQGLLFLNPSQVWLQPPGYVTQMISRNYQPLAVKTENPDHDLDVSAT
                     KSEDGKTLVLQVVNVSEKEATLPLKVSGFVPAEPVAQVLTLEGPLDAYNTASAPEAIK
                     PVETQWRHDLKDGETTVTIPAHSFTVIRL"
     gene            complement(11714..>11948)
                     /locus_tag="VG754_06780"
     CDS             complement(11714..>11948)
                     /locus_tag="VG754_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18955.1"
                     /translation="QIAKAYLAGTDSGVATRTWQQALDAIVETKSGSTQDCGRRAAKE
                     TALDSIRNRVILEAQAEHLFVCLIMYGITEICH"
BASE COUNT         2756 a         3051 c         3415 g         2726 t
ORIGIN      
        1 gatgcggccg gacattgggt cactgctgat tcttggtgcc ggtccgattg gtttgggtgt
       61 tctgatgatg gcgaaaatca tcctcggcaa tcatgtgaag gtttatctga cggatattat
      121 tccttatcgc ttgaagctcg cggagcaatt aggcggcctc ccaatcaatg ttgccaaagg
      181 ctccatcaag gacggcctga agtcgcacgg cgcggatgcc gctgacatgg cggtggacac
      241 gagcggtaaa caggtggcgc gcgaggaagg gcatcgcgcc ttggcccagc gcggttcttt
      301 gatttgcgtc ggtcacggcg aaggcatttc actcaaggtt tctccggaca tcatcgcacc
      361 cgagcgcagt gtggtgggta gcgagtattt tcgctttgat gaacttccgg ccaacttgcg
      421 tcggttgacc cagcatcgcg cttatctcaa tcaaatcatc acgcatcgtt tcggtgtgga
      481 cgaaattcaa aaatccttcg aactcttttt tggcggcgaa accggcaaag tcatcatcga
      541 acaatgagct caaaaattaa agccgtattg attggcgcgg gcggatgggg actgcaacac
      601 gcgcgcattt tgtcgcaacg ttccgacgtg gaattctgcg cgatcgcggg acgaaccttg
      661 gaaaaaacca agacccgctc cgggcagttt ggggtgcgcg catacaccaa catcacggag
      721 atgatcaaac aggagaagcc tgatttggcg tttctgtcgc taccgaacga cgggcatttt
      781 cagccaacga tggaagtgat ccgcgcgggt gtgccgttgt ttgtcgagaa accgctcgtg
      841 tttgatttga aagaagcgga caagctcgtc accgaagccg cgaaacgaaa actttttttt
      901 ggcatcaatt ttaatcatca ctatgctcgt ccggtggcgc tggcgcatca tgccattcag
      961 caaggacgtc tcggtgaatt gacttttgcg acgtggcgtt ttggcggcga aggcggcacg
     1021 agccatccgt ttagcaactt gcttgaaact cagtgtcacg ccttcgatat gctggaattt
     1081 ctctgcggcc cgattgattc ggtgatggcg cagatgaccg acaaaaccaa gaaaggattt
     1141 tatcgcacga tggtgctggc gttgcacttc aagaacggcg cggtggggag tttggtaggt
     1201 tcttatgatt cctcttacgc gtatcccgac acgcaccggg ttgaaatcaa cggcacgaaa
     1261 ggccgcatcc ttatcgagga cacggttcgc cgttacacct tcaatgctgc tggttcggag
     1321 acggccgagg tctggcagtc cggatatttc aatgatcggg accgtgattt ttaccttacg
     1381 ttcgaccgtc acatggatca cgtaatggac gcattcaagg ctggcaagca accacccgtt
     1441 cacgcccggg ctggacgtcg cgcgttgcaa ctggcttttg cgtcgatcaa atcatttgag
     1501 tccggcaaaa aagtttccgt gccgacagat tcatttgaga tggcggcagc gccggccatt
     1561 ttgccgccac cgccgaatcc ataaactggc tttcttagcg tattcacttg agtgccgaag
     1621 ctaccaatcc agccgtcagc tttcgtggca tcggcaagcg ctttccggga acggtcgcgc
     1681 tccagaacgt aagttttgac gtggccaccg gtagctgcca cgccatcatg ggcgagaacg
     1741 gcgctggcaa aagcacgctg ggaaaaattc tcgccggcat tcaccagccg agtgaagggt
     1801 gcataatggt tgaaggtgtc gaacggcggt ttcattcccc gcttgatgcc caacgggccg
     1861 gcgtagccat cgttcatcaa gagcttttgt tttgtcccaa tttaagtgtg gcggaaaaca
     1921 tctgtctctc gcatctgcca agccgcatgg gacggttgga ttggcccaag ctgtatcagc
     1981 gggcgcagac tttcttggat caagtcggcg ctgaatgtgg tgcgcatgaa gagttgggcc
     2041 gcttgagcac gggtcagaca cagttgattc aaatcgccgc cgcaatggcc accggcgcga
     2101 aaattctggt gatggatgaa ccgaccagtt ctctttcggc gatggaaacg caacggctgg
     2161 aggagatcat cgtgcgattg cgccaaaacg gggcgaccat catttatgtc tcccaccgca
     2221 tggacgaaat tttccgtctc tgcgacgccg tgaccgtgat gcgcgacggc cagcacgtcg
     2281 ccaccatgcc actggcgcaa accaacgagg aagagctggt caagctgatg atcggcagga
     2341 gctggcagaa aatgtttcct gagcacatta accaggcagc cgggccggaa cgtttgcgat
     2401 tggaaaattt ttcttcgctg ggaaaatttc agcacattaa tttttcgatc cgctccggcg
     2461 aagtgctggg cgtggccggg ttggtgggtt cgggccgcag cgaagttgca ctgggaattt
     2521 ttggcctcga cccgaaaact tcaggtcgcg ttttggtgga tggaaaagaa tcctgcccgc
     2581 gttctccgcg tgacgccatg aagctgggta ttggcttggt cagcgaggat cgcaaaggtc
     2641 aggggttggt tctgggcatg gagtgcggcg aaaacatcac gctgtcctct ttggatcgca
     2701 tcagtcgttg cggagtcatc cggcaaggca atgaacgagg aatcatcgcg caattttttg
     2761 aaaaacttcg cgtcagtgcc gcctcgccgc aagttttggc gcagacgctt tccggcggca
     2821 accagcaaaa gctggtgctg gcaaaatggc tggcgcgcga aagccggatt ttaattttgg
     2881 atgaaccgac gcgcggcgtg gatgtcgggg ccaaggccga aatccacaag ctcattgatg
     2941 aactggcggc agcgggctgc gccattttga tgatttcttc ggagttgccg gaggttctca
     3001 atctcagcac gcgggtcatc gtcatgcgca acggacggct cgccggggag ttgaatcgcg
     3061 cggaggcgac ggccgggcgt gtattgaaat tgatgaccgg ctgcggcgag gaaaaggaat
     3121 tacgtcctca aattttatct gatttggcgg tgacttgatt atgccatctg aacaacccat
     3181 tgccgccggt gcggaaccta tcgcccgaac tggacgtgtg accagtttca agcgttccag
     3241 attcaaggag accggcttgg tcggtgtgtt ggtggcgctg ggattattcc tttcaatttt
     3301 tggcggttcc ttgcaggtca cgaatcggga tacgggaaac gtcatcaagg tgaacaagtt
     3361 catgcgcgcg gacaatctcg acaaactcgc caagaacact tcgttcttcg cgatcatggc
     3421 ggtgggcgca acgtttgtga tcatcaccgg cggcattgat ctgtccgtcg gtgcggtcta
     3481 ttgcctcgcg gcagtttgcg gtgcgatgtt tctgacccgg tttggcccgc atggaatcgg
     3541 ggccgggact tcgccgttct tggtggttcc gggcgcggta ttggtgtgcc tcggaacggg
     3601 tgcgttatct ggttttatca atggcatcgg tgtcgttggc ctgcgggtgc atccatttgt
     3661 gatcactctg ggaacgatgg ccatctatcg cggcattgcg tttgtgatga ccaaggcgca
     3721 agcattcacg gattttccgg ttcaattcac cgacggattt atccgccgtg aaatcacctt
     3781 ccaaggggcc agtctttatc cagtgccgct ggccatcatg attggcgtga cggcatttgc
     3841 ctggtggttt ctcaaacact cagtgacggg acgtcaaatt tttgccgtgg gcggcaatga
     3901 gcaggcttcg ttgttcagcg gcctgccggt caactgggtc aaggtgaagg tctatacttt
     3961 cagcggcctc gcggcgggaa ttgcagcgat gatcatgctc ggctactacg gctccgcttc
     4021 ttcggatgcg ggcaagggtt acgagctgga tgtcatcgcg gcggcggtcg tcggtggcgc
     4081 aagtttgatt ggcggccgtg gcaccgcttt gggcgcactg ctgggagcac tcatcattca
     4141 agtcatcaac aatggtatta tcattctcaa tatagatcaa aactacagcc agatcatcat
     4201 cggcgcggtg atcgttctga ccgtgctgct ggatcggatc aataactcaa tgcaggaaaa
     4261 acgaatgacg cgcgcggctc aagccgcgaa atagcgttcc tgaatttttt aagaaagcac
     4321 aaccagacca aacatcatga acatcactca aatcatcact cggacggcac tgacgctgtc
     4381 ggcaatcttg attgtggccg gctgcgacca gaagaaaagc gacgtgacac cgtcggcggc
     4441 gagccccggt tcgaagaaaa ccgaatatac tttcgccatg gtggccaaga gccagtcgaa
     4501 tcccgttttc caggctgccc gcgtcggcgc cgaagatgca gcagctgatc tgagcaaaaa
     4561 actgggagtg aaaatcaatg tgctttggcg cacgccgaac gaagacgacg cgcaaaagca
     4621 ggttgaaaac atcgaacagc tggtgttgca aggtgtcgat ggcatcagcg tcagttgcag
     4681 cgaagctgaa accttgacgc cagccatcaa caaggcggtc gagagtggcg tggcggtgat
     4741 gtgctttgac agcgatgccc ccgccagcaa gcgtttttgc tatcatggca cggatgacat
     4801 tgagtgtggt catatcatca tgcgggaact ggcgacagtg ttaggaccag gcaaacacgt
     4861 cgtggccatt cccggcggaa atcaaaatgc ccgcaacctc cagaatcgca ttaaaggcgc
     4921 agtcgatgag gccacaaatt atcctaacat ctcggttcgc ggcatttatt acagcaaaga
     4981 agtcccgcag gacgccgccg ccaagattga agaagttcag acggcgaacc ccgacattga
     5041 cggctgggcg atgattggtt cgtggagctt gtttaccgat gccttgctga aatgggagcc
     5101 gggcaaggtc aagatcgtgg cgatggacgc gctgcctgcc gaattaccct atctgaccaa
     5161 aggcgtcgtc cagaaacttt acgcccagca gacctaccgc tggggcgtgc gttcggtgga
     5221 attgctggcc gacaaagtca tcctaggaaa aaatccggcg aatgtgatgg attattcgga
     5281 gctgcctcct gtcacgaagg acgacgtgca acaatacgaa caaaactgga agaaatggct
     5341 tcgccaatga caggtgtagg ttccggccat gaagcaaaga attaacgtga aaacatttct
     5401 ggcattattg gcagtggctt caaactttgc attcgtttca ggtagtgcaa aagcccaaat
     5461 ggcgaacatt tatgtcaata ccagccagac acttcacacc aattccgctt atctgactgg
     5521 cgcgtgtttg gaagatgtga accatgagat atacggcgga ctctatagcc agatggtgtt
     5581 tggtgaaagt tttcaggagc cggcctcgcc ccggcctttg actggattta ccgcctacgg
     5641 cggcagttgg ctgccaacga acggcgtgct acaggcggcc gccggcagcg ggcccaagtt
     5701 gatttataat gggtttaatc agacgaacgg agatgtcagt gtgcagttgt ggttttccag
     5761 caacgccggg ggtaacgccg gactgattgt ccaagtcagc caacctggcg tgggcgcgga
     5821 tgtttttaac ggttacgaaa tttcgctggc accggcgggt tatctggtgc tgggtcgcca
     5881 cgtccaaaat tttacatcat taagcgaggc gtcctgttca gttcccatag gtcaatggat
     5941 tacgctggag gtgcaatata ccaacgcaac cttcaacatc ctggtcaaca gcaacagtat
     6001 ccttcaatat actgacacac agcaaccgct caccagcgga caagcaggtt tgcgcacctg
     6061 gcagcaaagt gtgcaatttc aaaatttctc catcaacacc aacatcaaca tcccatttca
     6121 gcttgccacc aatggtttgg ctgaaggcgt gagcggcatg tggaatcccg tcagcaccgg
     6181 cacggttacg gggcagttcg caatggagac gaccgatccg tttgtcggga cgcaaagtca
     6241 gcgcatcacc tttaccagtg gaactggggt ggttggcatc gccaaccagg ggctaaaccg
     6301 ctgggggatg cccttcgttg gcggcagctc ttacaccgga acacttgatg cgcgggccga
     6361 tgcaccgacg accatatggg tggctttgga gagcgccgat gggtcttcag tgtatgccga
     6421 acaaagtctt tccgtgagca gtaacaactg gcagcagctt aatttcaacc tgactccgtc
     6481 tgccagcgac ggcaacggac gctttacgat caagctgaaa caacccggtt cgatggtggt
     6541 gggctatgcg tttcttcagc ccggaccctg gggctgcctt caagggttgc cggtgcgcaa
     6601 agatgtggtg caggggttga tcaaccaagg cgtcaccgtt ttacgctatg gcggttcgat
     6661 ggtcaatgcc tcggggtatc gctggaaaaa tatgatcgga tcgcgtgatc gtcgcccgcc
     6721 ttataccggc acatggtatc cgtattcctc ggatggctgg ggtattccgg atttccttaa
     6781 tttctgtgaa gcggccggat ttcttggggt tccggatttc aacatcaacg agacgccgca
     6841 ggacatggct gatttcatgc aatacgtcaa tggttcgacc aacacggttt ggggcgcgca
     6901 aagattggcg gacggtcatc cacaacctta caacttgaaa tacattgaac tgggcaatga
     6961 ggaggcggtg aacagcacct attaccaaaa attccaggcg ctggcccaag ccatttgggc
     7021 tgcggatacc aacgtcatcc ttgtggtcgg agatttttcc tatcatcagg tcattaccaa
     7081 tcctttcagt tttagtggtg ctgattcggg catcacaaca ttggcggcgc agcagcagat
     7141 tttgcaactg gcccagacaa atagtcgggc agtgtggttc gatgtgcatg tttggaccga
     7201 cgggccgacg ccggacagca gtctggcggg gatgttttcg tatgacacag cgctggggca
     7261 gattgccaat ggcgcgaatt acaaggtagt ggtgtttgaa ctgaacgcaa acaatcactc
     7321 ccagcggcgt gctttggcga atgcgctggc gatcaatgcc attgaacggg atgggcggct
     7381 gccgatcacc acgtctgcca attgtttgca gcctgacggc cagaacaaca acggctggga
     7441 tcaggggttg ttgttcctga accagtccaa cgtctggttg caaccgccgg gctatgtgac
     7501 ccagatgtat gctacgaatt ttcaaccgct ggaaattcag agcagtgtta ccgacccaaa
     7561 taatgatttg gatgtcagcg ctgaatgtag tcaggacgga agtaaactgg tgttgaaggt
     7621 ggtcaatctc aacagttccc cggaatccgc taccattaat atttccggtt tcgcgccaac
     7681 gaaccctgtc gctgccgtcc aagtgctcgc ttcattacaa agttcagtca acaccgcgca
     7741 ggctcctttt ggattcgtgc ctgcgaacat cagttggcag cacaatttta atggcagcaa
     7801 cgccaattat acatttgctg caaactccgt caccaccatc acttttcaag ggcaagtgat
     7861 agtgcccccg ccgactccac cggtggttct tactcatcgt tggagcttta acgaaccggc
     7921 caactccgct cagtttgttg attctgtgct tgggatcacc aatggcatta ttcacggagg
     7981 tgcccgcatt gatgggaatg gaaatctcgt tttggctggt ctaaaccaga aaacgaatta
     8041 cgcggagttg ccgccttatc tgctcaccag caccaattat actgcggtga catttgaatt
     8101 ttgggttgca tttggaacca ataatacttg gggtcgcctg attgattgtg gcgacacaaa
     8161 ccctaatacc ggtaatggtc gttactgtct ggattttacc ccgcatagtg gttatgggcc
     8221 caacggcatt aattttgaag tgtcgggcac cgaccccggc aattcctcag cccaaaatat
     8281 agccgtgccg cccgtattgg ataatcaggg caagatacac ttggtgttga tttgggattc
     8341 cattgccagg tatatggctg tttacaccaa tggcgttctc atgggaatta acagtagtgt
     8401 gaccctgcct atttcggcca tcgtcaatgc gcatagctat ctcggcaaat cttcatatac
     8461 gggtgactat tgtggcgtgg ccacaatcga tgagttccgg atgtatagcg gcgccatggg
     8521 gatggcgcaa attgccgatg attatgcctc gggccccaat tctttgcccg tgccaaaact
     8581 gtctatcgca accgccgggc aaaatctggc gttcacttgg ccggattata ttaccggtta
     8641 ctcacttcaa acttccgcca aacttggagc gggtgcatcc tgggtgccgg cagcggtgtc
     8701 gtcatatttc ctgacgaatg ggacattttt gataaaagtg cccatgacca gtcagcaggc
     8761 attttatcgt attgtcaaat aggctgcctg gaagaaatca aaacaaaaaa atgataaatc
     8821 aaaccggttt gataaaagcg gccgccatct tggcgggaat tctcagtgtg cttgcgacgg
     8881 ttggtcagac taaaggtgcc accgtcactg ttaatgcgga tcaagtgttg cacaccaatt
     8941 cgccttatct gaccggggcg tgcatcgagg atgtgaacca tgaaatttat ggcggcatag
     9001 acagccagat gatttttggc gaaagctttg ccgaacccgc ggcacaactg ccgttaaaag
     9061 gatttaaagc ttacgagggc cgctggacgc tggcggacga tggcagtgtt cagggtgttg
     9121 gcagtaatgg ttcgaaaatt gtctgggacg gcccggcgtt gtccgtgggc gaggtcagtg
     9181 tggacgtgaa gctgacggaa gcaaccggcg gtaatggagg tttgatactc aaggtcagcg
     9241 atgctggcat aggcgcggat gcgttcaccg gctacgaagt ttcgcttgaa aggccggggc
     9301 agttggtggt gggccggcat cgtcaaaatt gggagccaat ccgcaacgtg ccgtgcgatg
     9361 tgccggttaa tgaatggatc aagttgaccg ttcgcgcgac tgcaaagtcg tttgaagtgt
     9421 tcgtcaacgg caagagcatg ctgcaattcg aagacaccga gcatccgatc gaaaccggca
     9481 cagtgggttt gcggatctgg cagcacaagg tctcgtttcg caatctctcc gttgccaccg
     9541 gtgcatcgca gcagaaggtg gcgtttgact atgacgctgg caataatcca tcggacagcg
     9601 tgagcggcat atggcgcccg atccggcagg gcgcggtcaa gggaagtttt tccctggaat
     9661 cgcagggtgc attttctggc aatcaaagcc agcaaatcac ttttgccggc ggttcaggtg
     9721 ccatcggaat ggagaaccaa ggcctcaatc gccggggcat gaattttgtg aaaggcaaaa
     9781 actatgaagg ctatctgtgg gcgcgggcca aatcgccaac ggaaatattt gtgactttgg
     9841 aaaacacgaa tggctctgtg gtgtatgccg aaaaatccat gaagctcaag gctggcgact
     9901 ggcagcggct cgatttcgag cttacgccga acgcagtgga ccaggccggg cgctttgcca
     9961 tcaagctcaa gcgagccgga tcggttactg tgggttacgc ttttctgcag cccggctctt
    10021 ggggacgttt caagaatttg ccggtgcgca aggatgtggc tgaagggctg atcaatcaag
    10081 gcgtgaccat cttgcgctac ggcggcagca tggtgaacaa ttctgagtat cgctggaaaa
    10141 agatgaccgg gccgcgctct caacgtccgc cgtatgaagg tcattggtat ccgtattctt
    10201 cggacggatg gggcattttt gatttcttga acctatgcga agccgcccga atcgtcggca
    10261 ttcccgacct cgacatcaac gagtcgccga aggacatggc tgatttcatg gattacctca
    10321 acggcacacc ggacagcgag tggggaaaaa aacgtgtcgc cgacggccat ccggcgccat
    10381 atcgtttgaa atacatcgaa ctgggcaatg aggagcgcgt tgatgataac tactttgaaa
    10441 aattcaaggc cgtggccgaa gtgatttggg cgaaagaccc cgccatcgtt ttgatcctgg
    10501 gcgatttttc ctatcacaaa gtaatcacca ccccgttcaa cttcaccggt gcggacagcg
    10561 gcatcacgac cttggccgga caacagagaa ttctacaact cgcaaagcag catgaccgcg
    10621 aagtatggtt tgatctgcac gtctggacgg acggcccacg accagacagc agcttggaat
    10681 ccatgttctc ctacattgat gcgatggaca aaatcgccga cggcgcgaaa cacaaagtgc
    10741 tcgtcttcga gttgaatgcg gacaatcact cgcagcggag ggcgttggcc aacgcgctgg
    10801 ccataaacgc cattcagcgc gacggacgcc tgcccatcac ttgctcggcg aattgtcttc
    10861 agcccgacgg ccagaatgac aacggatggg atcagggttt gctgttcctc aatccctcgc
    10921 aagtgtggtt gcaaccgccg ggctacgtga cgcagatgat ttcacggaat taccagccgc
    10981 tcgcagtgaa aaccgaaaac ccggaccacg accttgacgt cagcgccacg aagagcgagg
    11041 acggaaagac gcttgtcttg caagtagtga atgtgagcga gaaggaggcg acgcttccgt
    11101 tgaaagtttc cggctttgtt ccggcggaac ccgtggcgca agttctgacg ctggaaggtc
    11161 cgctggatgc atataacacg gctagcgcgc ctgaggcgat taaaccggtg gagactcaat
    11221 ggcggcatga tctcaaagac ggcgaaacca ctgtgactat ccccgctcac tcattcacgg
    11281 tgatccggct gtaaaattga aaatgaaaca acaattgaac cgcttgtcgg cccttgagtg
    11341 cgaaacgtcg catcaggaaa aattgcaacc agaaagcacc cggggctagg ttgcaagccg
    11401 agggctggga tgtcagccga gaatctctag ccaagctgga atcacaattt cggcgggtgc
    11461 gggattacgc cttggaaacg tccaccaaat ttccccgccg caatctcagc cggtcgcagg
    11521 cgatgatttt tttggattct gatttgtttc cagatagaca ctttcccgcg cgaaatccta
    11581 aacgaaaaag ccccgagccg ggcggcggat caaggtggaa gccgaggaca taccgaggaa
    11641 cgaccttgac gcgacgaccg gcgacataca atggaaaccg aattctcgct taacaggatg
    11701 gattccccgg cattcaatgg cagatttcag tgatgccata cataatgagg cacacgaaca
    11761 gatgctcggc ctgcgcttcc agaatcacac gattgcggat ggagtccaac gcagtctcct
    11821 ttgccgcccg ccgcccgcaa tcctgggttg agccgctctt ggtctcgaca atggcatcaa
    11881 gggcttgttg ccatgtccgg gtcgcgacgc ctgaatccgt tccggccagg tatgccttcg
    11941 caatctgg
//
LOCUS       DASZMM010000281         6175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2028498, whole genome shotgun sequence.
ACCESSION   DASZMM010000281 DASZMM010000000
VERSION     DASZMM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028498"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VG754_06785"
     CDS             complement(<1..140)
                     /locus_tag="VG754_06785"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HWF18956.1"
                     /translation="MWSVLREFLRFCAQEKKWWLIPLVVIFLVLGLVLIFASSTGISW
                     AL"
     gene            complement(380..2641)
                     /locus_tag="VG754_06790"
     CDS             complement(380..2641)
                     /locus_tag="VG754_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HWF18957.1"
                     /translation="MLKAAPPAGYYEVWGDEFNGTSLDTTKWDYWVPGSWGNAVNVSN
                     AVTVNGSNLVITTYTANNTNYTSIIASDNHFRPRYGYYEASIKWGDTNGEWSAFWLRS
                     PTMGTWLDDAFVSGAEMDACEHRYVGIYGTTNDNIVSDNIHWDGYGAAEQNAGSPNVG
                     NNLQIGYHTYGLLWNGATYSFSIDGSQVWDGTPAPLFGSDVYVILSSQVDDTSTTWAG
                     YIPAGGYDSQAASVIKLSVDYFRYYAPTNVLFWTGGTSAYWTNSANWVSNMIPLANSD
                     LTFSYLTVNPNPVVGSNYSVDGLVFLNMTNSPSIGGTNTLTLGAGGIDMVAANHNATL
                     NAPINIGANQRWTVGRNNPGNLLTVNSDLSGTATLTKAGYGTLVLKGTNSFSGTLNVD
                     TGSSVTNDGALLIGNNAAISSVASISVRNGGTGVSTLQLSNVTVLPVVNIVARSTNVP
                     SIDAVSGTTNTLAGGVNLTGGGSNYYIQCDSGATLKLSGSISATTTSPCTLFLGGNGS
                     ISVPTSIQNGSASVMNVVKTNAGVLTLSGANTFNGSMTSYNGTLYVNGTVPGQLTANS
                     GILAGNGNVVGDATVAGGEICPGTSVGNSIGTLSFGGNLTLGAHILALMDINAGTQTN
                     GQLNVAGTLTYGGTLNVLNLGGTLAPHQSFKLFSASNYAGGFSGLNLPALNSGLQWST
                     NGLANGVLSVVALPQTISVMNQGSGQMQLSWQYGTLQTATNVAGPYTNVAGATSPFTI
                     SPTNLQQFYRLSE"
     gene            2864..3790
                     /locus_tag="VG754_06795"
     CDS             2864..3790
                     /locus_tag="VG754_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18958.1"
                     /translation="MEKKENKDAAMETAGANHSRTREQRGFTFVELLVVIVVLALFAA
                     LSLQRLAKSKTQAFQCLNNHRQLISAALIYADESSGIWIPNEPTGTGNQTDWVNVNMD
                     WTASNTDNTNYNKLIDPHSSLLAPYLGGNPNIFHCPSDQSFVLNEGPRVRSVSANHAV
                     GSVWVTTGCCIANGPVNGQWLTGANIGSACQTVYRCYGKSTDFTLPGPSQTFVFTDEH
                     CDSINDSVLAFQCGQQGIGAAFIDRPANYHNGAAPFSFADGHLEMHKWLGASLGAGPD
                     DWNDFSNYSLNVTTSADNSDLIWIQQRTSAHK"
     gene            complement(4282..5196)
                     /locus_tag="VG754_06800"
     CDS             complement(4282..5196)
                     /locus_tag="VG754_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF18959.1"
                     /translation="MTAYKSCQTRARRQAFTLIELLVVIAIIAILAALLLPALASAKA
                     KAQRIQCNSNLKQLGLGFITFANDNGDMYPPAAMSSQSTEGQLAWDDWIHRYIGGNAS
                     QNTLIRADGLLDPGDCPKIEECPADRLPIIAWAQDYAQRRSYAMNSVGPNYATQWQVD
                     PKNRTYPLPTPVHGIGIYWDDPGSAPVDWDAKGYKTDVVLDASGTFLLVEEPNEQNVV
                     GNVWPSICNGPVSSSSGGNDDLYQTALGGGSGGNGQNYGNSDYGLHSGRFNYLFFDNH
                     VQAHKMAETVGSGTLANPMGMWTLAQGD"
     gene            complement(5227..5592)
                     /locus_tag="VG754_06805"
     CDS             complement(5227..5592)
                     /locus_tag="VG754_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18960.1"
                     /translation="MNPVVFVALLVLLLAGCKRHETASNTGTIAANQSQTTTDAPVPP
                     SGPPTQAPSEPVVVAENSDVNAILDQLSAELRVYVSRTRSAPKDFQDFVNRAQLQAPA
                     PPAGKSYAIARGKVVLVNQ"
     gene            complement(5654..>6175)
                     /locus_tag="VG754_06810"
     CDS             complement(5654..>6175)
                     /locus_tag="VG754_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF18961.1"
                     /translation="ARELTRAMDEIVWAVSPQHDTLDSLVTYLGKFAQDFLSVAGIRC
                     RLNVPIELPAWQLTAEVRHNLFLAFKEVLNNVLKHASATEVRITLTPSPTGFWLVVDD
                     NGKGFDVTRNGAVDSQAATDHGLRFATGNGLTNVKKRLEEISGRFELKSAQGEGTTVR
                     FWIAVRTDVRHQN"
BASE COUNT         1444 a         1671 c         1629 g         1431 t
ORIGIN      
        1 tacagcgccc aggaaattcc ggtgctcgac gcgaagatca ggacaaggcc cagcaccagg
       61 aaaatcacca ccaacggaat cagccaccat tttttttcct gcgcgcagaa ccgtaggaat
      121 tctctaagta ctgaccacat aactctgcga ttctagcgac gacaccacgc caagcaaagt
      181 caattatgaa gggaaactgt aaatataaca tctcataaat gaagaacgga gcgcgactgt
      241 gtccgcagga ccagtcgcag cagcttggga agactcagga ttcagtagct gctgcggttg
      301 gcggttcgcg cacagccgcg ctccactatt tatgagatgt gctgaaatct ttgagcctat
      361 gcccgcgtta attggtgaat cattcgctga ggcgataaaa ctgttggaga ttggttgggg
      421 aaattgtaaa cggcgaggtg gctccggcga cattcgtgta aggccctgcg acgttggtgg
      481 cggtttgcaa cgttccatat tgccagctca attgcatctg gccgctgccc tggttcatca
      541 ctgaaatcgt ttgaggaagc gcgacgacgg acaaaacacc gttcgccaat ccgttcgtgc
      601 tccattgcag cccggaattc agcgcgggga gattcagccc ggaaaatcca ccggcgtaat
      661 tgctcgcgct gaaaagtttg aaactctgat ggggcgcgag cgtgccgccc aggttcagca
      721 cattcagcgt tccaccgtaa gtcaacgtgc ccgccacgtt caactggccg tttgtctgcg
      781 tccccgcgtt gatgtccatc agtgcgagga tgtgcgcgcc gagcgtgaga tttccgccaa
      841 acgaaagtgt gccgatggaa ttgccgaccg aagtgcccgg acaaatttca cctcccgcaa
      901 ccgtggcgtc gccaacaaca tttccgttgc ccgccaaaat tccgctgttc gccgtgagct
      961 gcccggggac tgtgccattg acatacaacg tcccattata ggatgtcatg ctgccgttga
     1021 acgtgttcgc gccggacaaa gtcaacacgc ccgcgtttgt tttcaccacg ttcatcaccg
     1081 acgcactgcc gttctggatc gaggtgggaa cgctgatgct tccattcccg cccaagaaca
     1141 acgtgcaagg actcgtagtg gtggccgaaa tggacccgct caacttgagc gttgccccgg
     1201 agtcgcattg aatgtagtaa ttcgaccctc cgccggtgag attcacgccg ccggccaaag
     1261 tattggttgt gcccgaaaca gcatcaatgg aaggaacgtt ggtgctgcgc gcgacgatgt
     1321 tgacaaccgg caaaacagtg acgttcgaca attgcaacgt ggaaactcct gtgccgccgt
     1381 tgcggacgga aatcgaagca acgctgctga tggcggcgtt gttgccgata agcaaagcgc
     1441 cgtcattcgt gacgctgctg ccggtatcaa cgttgagcgt gccggaaaag ctgttcgttc
     1501 ctttcaaaac caatgtgccg tacccggcct tggtcaaggt ggccgtgccg gaaaggtccg
     1561 aattcaccgt caaaagattt ccgggattat tgcggccaac ggtccatctt tgattcgcgc
     1621 cgatgttgat gggcgcattc aacgtggcgt tatggtttgc cgcgaccatg tcaatgccgc
     1681 ccgcgccgag cgtgagcgta ttggtgccgc cgatggaagg cgagttggtc atgttcagga
     1741 acaccaaacc atccacgctg taattgctgc ctacaacggg gtttgggttg acggtcaaat
     1801 aggagaaggt caaatcgctg ttcgcaagtg gaatcatgtt ggaaacccag ttcgcggagt
     1861 tggtccaata agccgatgtt ccgccggtcc aaaagagaac attcgtcggc gcgtaatatc
     1921 gaaaataatc cacgctcaat ttgatcacgc tcgccgcctg gctgtcgtaa ccgcccgcag
     1981 gaatgtaacc ggcccatgtc gtcgaggtgt catccacctg gctgctcaaa atgacataga
     2041 catcgctgcc gaacagcggc gcgggcgttc cgtcccaaac ttgcgaacca tcaatcgaaa
     2101 acgaatacgt cgcgccgttc cataaaaggc cgtaggtgtg atagccgatc tgaaggttgt
     2161 tgccgacatt cggacttccc gcgttttgtt ctgccgcgcc gtatccgtcc caatggatat
     2221 tgtccgatac gatgttatca ttggttgtgc cgtagatgcc gacgtaacga tgttcgcacg
     2281 catccatttc cgcgcccgaa acgaacgcgt catccagcca cgtgcccatc gtcggcgacc
     2341 gcaaccaaaa cgccgaccat tcgccgttgg tgtcgcccca cttgatgctt gcctcgtaat
     2401 agccgtaacg cgggcggaaa tgattgtccg acgcgatgat cgaggtgtaa ttcgtgttat
     2461 ttgcggtgta agtcgtgatg accaggttgc tgccgttcac ggtgacggca ttggaaacat
     2521 tcaccgcgtt tccccacgac ccgggcaccc aataatccca tttggtcgtg tcgagcgaag
     2581 tcccgttgaa ttcgtccccc cacacttcgt aataaccggc cggtggcgcg gctttgagca
     2641 aatttaaacc cagaaaaaaa atgaaaagaa gaactgtcgt gacaacagcg ccgtaacgtg
     2701 atttctgcct tctgggtttg tgcaacaagg taatttaagc agaataaatc accaatttca
     2761 agaatgaaca agcaccctgc gcgcttgcca gcgtcaggca tcccgcctga cttagcggac
     2821 gcatgttccc gccgagcagt gtgatgcgaa aaaacttgct tttttggaaa aaaaggagaa
     2881 taaagacgct gccatggaaa cggctggagc aaaccattcc cgcacccgtg aacaacgagg
     2941 gttcacattc gttgagttgc tggtcgtgat tgttgtcctt gcgctttttg cagctctttc
     3001 gctccaacgg ctggccaaat caaagaccca ggcctttcaa tgcctgaaca accaccggca
     3061 gctcatctcg gcggcattga tttatgcgga tgaatccagc ggcatttgga ttcccaatga
     3121 acccactggc accggcaatc aaaccgattg ggtgaatgtc aatatggatt ggaccgcaag
     3181 taatactgac aataccaact ataataaatt gattgatcct cattcttccc tcttggcgcc
     3241 atatcttggg ggaaatccga acatattcca ttgtcccagt gatcagagtt ttgtcttaaa
     3301 cgaaggaccg cgcgtccgga gtgtttccgc gaatcacgcg gttggttcgg tctgggtcac
     3361 aaccggatgc tgcatcgcaa atggtccggt gaatggtcag tggcttaccg gagccaacat
     3421 cggcagcgct tgccagactg tttaccgttg ttacggcaaa tccactgatt ttactctgcc
     3481 cggcccatca cagacttttg tgttcacgga tgaacattgc gacagcatca atgattcagt
     3541 tttggccttt caatgtggcc aacaaggtat cggcgcggca tttattgatc gccccgccaa
     3601 ttatcacaac ggtgctgcgc cattttcctt tgctgatggc cacttggaaa tgcataaatg
     3661 gcttggagct tctcttggag ccggccctga tgactggaac gacttctcta attacagcct
     3721 gaatgttacc acttctgcag ataactcgga cttgatctgg attcaacagc gcacttcggc
     3781 ccataaataa ttttccagtg cgctgaagta acattcaccg tccatcgaat ccaaaaattc
     3841 ggcgaaattt cttgccttca aaaatgcctg tgatagtctc attgccgttg ggaggaggcg
     3901 ggcaagttct ttgacattct aaaatagttg acggttgatg gacgaaaatt ctgccgaaac
     3961 tcgcgttttg actcagatcg tcccaagttg cctcagtaac cgggcttgac cgggctaaac
     4021 gtaagtagtt gagattccgc aaagtttcat aaagtttcat aaaatcccgc gcaaaaatac
     4081 cccctccccc cgggggctgg aacatcgaac gtccaacatc gaacgtcgaa cgctgaaatg
     4141 gaaaaaatgg ccctgaagtg tccgccggaa cattggatgt tcagagttca gcgttggttg
     4201 ttcgatgttt tccaactgtg tcgcttaatg tcgcttgcgg tggcacagtt ccagatcgcc
     4261 gaagcgcgcg gcatttccga ttcaatcacc ctgggcgagc gtccacatgc ccatgggatt
     4321 ggccaacgtg ccggagccga cggtttcggc cattttatgt gcttggacgt ggttgtcgaa
     4381 aaacaggtag ttaaaacgac cgctgtgcag gccgtaatcg ctgttgccgt aattttgacc
     4441 attgcctccc gatccgcctc caagggcggt ctggtacagg tcgtcgttgc cgccactgct
     4501 tgagctgacg gggccattgc aaatgctggg ccagacgttg ccaacgacat tttgttcatt
     4561 tggttcttca accaggagaa aggttccaga agcatcgagc acgacatcgg tcttatagcc
     4621 cttggcgtcc caatccaccg gtgcggaacc agggtcgtcc caataaatgc cgatgccgtg
     4681 gacaggagtc ggcaacggat acgtccggtt cttgggatca acctgccatt gcgtggcgta
     4741 attaggcccc acgctgttca tcgcgtaaga gcggcgctgg gcataatctt gcgcccatgc
     4801 aatgattggc aggcggtctg cgggacattc ttcaatcttt ggacaatctc ccggatccag
     4861 aagtccgtcc gcacgaatca gagtgttctg cgaagcgttg ccgccgatgt aacgatgtat
     4921 ccagtcatcc catgccaact ggccttcggt ggattgcgaa gacattgcgg ctggcggata
     4981 catatcaccg ttatcattgg caaaagtgat aaagcccagc cccagttgct tcagatttga
     5041 gttgcactgg attcgttgag ccttggcttt cgcgctagcc aaagctggaa gcaacaaagc
     5101 ggccagaatg gcaatgatgg cgatgacgac aagcagttca atcaatgtga acgcctgtcg
     5161 tcgcgcgcgc gtctggcatg atttatatgc ggtcatacaa actccagcag tttgcgattg
     5221 ggtcggttat tgattcacaa gcaccacttt acccctggca atggcatagc tcttgccggc
     5281 tggcggagcc ggcgcttgca attgggcacg gttcacaaaa tcctgaaaat ctttcggagc
     5341 acttcgtgtc cgggaaacat agacacgcaa ctccgccgaa agctggtcaa ggatcgcatt
     5401 cacgtcggag ttctcagcca ccacgaccgg ttcgctcggt gcttgcgttg gaggaccgga
     5461 cggaggaaca ggagcatccg ttgtggtttg cgactggttc gcggcgattg ttcctgtgtt
     5521 tgaggcagtt tcgtgccgtt tgcaacctgc gagaagcaag acaagcaatg ccacaaaaac
     5581 gacagggttc atggcatcta gatactcccg gacaagcacg aacggcaagc ggaagaagct
     5641 ttaatgcctg tcatcaattt tgatgacgca catcagtccg caccgcgatc caaaatctga
     5701 cggtggtgcc ttcaccctga gcgctcttca actcgaacct gccgctgatt tcttcgaggc
     5761 gctttttcac gttggtcaaa ccgttgccgg tggcaaaacg cagcccatga tcggtcgccg
     5821 cttgggaatc aactgcgccg ttgcgggtga catcaaagcc ttttccgtta tcatcgacca
     5881 ccaaccagaa gccggtcggc gatggcgtga gcgtgatgcg cacttctgtt gcggacgcgt
     5941 gtttgaggac gttgttcaat acttctttga acgcgaggaa aagattatga cgcacctcag
     6001 ccgtcaattg ccacgcggga agttcaatgg gaacattcaa cctgcaacgg atgcccgcga
     6061 cgctcaaaaa atcctgcgcg aatttcccca agtaagttac caggctgtcc aaagtgtcgt
     6121 gctgcggact gaccgcccaa acaatttcat ccatcgcgcg cgtcaattca cgggc
//
LOCUS       DASZMM010000282         9626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2584008, whole genome shotgun sequence.
ACCESSION   DASZMM010000282 DASZMM010000000
VERSION     DASZMM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584008"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..2395)
                     /locus_tag="VG754_06815"
     CDS             complement(209..2395)
                     /locus_tag="VG754_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 71/99-like protein"
                     /protein_id="HWF18962.1"
                     /translation="MRNLRKLCGGFAVWFLLGAGVLARAQLAATTWNPAGNPTQPSDD
                     IWTTHGNWSGGVVPTNGYKAYFNAGAAPCIVNSVVGGCQVTIGDGGPGGVLIVTNGGS
                     LSAGDTTAGNNDNYAWTAIGYSNTGEMDIENGGSITFNYHLWIGLNPGSVGTLIMNGG
                     TASVAGAFGLGFGGGRGIMHINGGTLTLSQWATNSIQGSGSVLDVGGGTVVIAGNQLA
                     SVNGFIASSKITAYGGTGTVACNYNGTANTTTITATEGSSASSNTAYLSIQLSGTNLV
                     LSWPTSSVYYGLQSTTNLIAPVVWKSLTNTVITTNGTNDVILPLSSQTAFYSLNHGVD
                     ATTMKGKLLMGYQGWFACPNDGSAPNQWWHWFHNQTPTAANVNTDFLPDTSEFTSNEL
                     FNTGMTYSNGSPVQFYSSAVQETVLRHFQWMQENQLDGVFLQRFLADLSSPQFYSFRN
                     QVTANVRAGAEAYGRVFAIMYDVSGQPTNTLISNITNDWLYLVNTMHITNSTRSLCHK
                     GKPVVAIWGFGFNDGNHLASPQQAQTVINWFKSQGVTVIGGVPTYWRTLQGDSYTNAA
                     WTPVYLSFDIISPWAVGRFGDNAGADSYTANITIPDLAECKSNGIDYMPVVFPGFSWY
                     NENGGTLNQIPRNGGSFYWRQVYDDVHAGCNMIYGAMFDEVNEGTAMYKLVPTAAQLP
                     AQGKFLALDCDGITLNSDWYLRLANQGGKMLRGEIPLQTTVPILPP"
     gene            2850..4184
                     /locus_tag="VG754_06820"
     CDS             2850..4184
                     /locus_tag="VG754_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF18963.1"
                     /translation="MKQMMNSSRMFKSLLLASLLAGPMLAFNAMAQNREDINWAPSDI
                     FTDGNGNQYAYWDDANNWVGGVVPVVYDTNQANTFYNAAFDSDTVTCVVTNDAQVGQL
                     MAGFGGGGTLEITNGAQFQAGFSFGGIWTGIGFVAGPGTLIVDPGSDFTCASHLWVGQ
                     GTANQGTVIINGGTLHIPNGQLGVSWNGIGGTNYITITNGGALYMSQWSSQTLGAPGS
                     SSTNIGIMDIEANSQVVITNNQLGYMNTLETNGQLIAFGGLGKISAVYNPSANVTILT
                     GLAPSGSDTPVFSLQPSNVIVTLGGTATLTATASPATGYQWLFNSAPLADGNGISGSK
                     TATLTVANFNVAETGIYSVVATNANAASQNDRNYASSQGVSVTGESFTLYPVVTINGV
                     NGNTYVVQYTTSLTQPVTWTSFSTNTVGAGPLYVVDTNSPLSMKRFYRVVQP"
     gene            complement(4620..5504)
                     /locus_tag="VG754_06825"
     CDS             complement(4620..5504)
                     /locus_tag="VG754_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF18964.1"
                     /translation="MFRSAHANPHILNRKSKRGNGFTLIELLVVIAIIAILASLLLPA
                     LTQAKQRAQGISCVNNMRQLQIASIVYASDNNDTIAYNEGHPDSGSVIGIAPSAPNWV
                     AGSFGSTANPVASPLGAETNIYLLGVLGDNVPGVGQLVGSIGSYAKGAGVYHCPADKS
                     VDPASKALRVRSCSANCYVGTSPLAIKYDPGEILPGWAVFSKYADFNSVLGSSDCIQF
                     LDENPVTLNDGFFLLKPTALNDRPAVNHGNATSFSFADGHAQLKKWQDVFLNANSTTS
                     GPTTDNQWLTAHATYKVN"
     gene            complement(5587..8577)
                     /locus_tag="VG754_06830"
     CDS             complement(5587..8577)
                     /locus_tag="VG754_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18965.1"
                     /translation="MNFKIHSLHVSTTKVLTALIMGMGVVCLVLGVPSTQANPAINNV
                     YPDGAHLFEPSSTLAFNAVSSVGVSNISVQLTATKLTGGSPFLRTLSPGNGLAVSGPS
                     TNESASCSLSSNRFYTAVIQVTDNNGGSTSTTVTFDTIVAGFTFEAEDFDYTSNSISG
                     QFIDNPQTNAYAALSATDGIDCHHDGSGNHDYRPNPPGLASESNSDVPRVQYMGTGKT
                     DYDIGWNNGGDYGNYTRHYPPGVYNVYMRGSDGGGPSSDSASLTVYSGSPTLTGSGPF
                     TWAVPPLGSWQTYSYVPLKDSSGNFAQLTIPNDGTASTLRVTIDQGSVNANFYLLMPV
                     DTNVSAASDAIITNIYPDGNYQFQMTNALAFSIVSTLGVNSNNLTVQFSATNLSGTGS
                     SILLSSGNGLTVSGPSTNLSVSIPLTSNMVYTAFIQVIDANGTPASTTVTFDTIIPFY
                     TFESEDFDYGNGQFFDNPQANAYQYMDGVSGVDFTNSNPAQYQYDRIGLSTETASDVA
                     RLSHAGFQDYDLGNSVGGNWGNYTRTYPAGIYNIFLRAADGNGTTSNSCSMSLVTSGW
                     GTSSQTMSKLGIFSVPASGGWQKYGWVPLKDSAGNLAQFAGGSTNTLRFTTDNGNYNA
                     NFFLLMPADNTIKVLPRVDNFTPDGSALFQYTNELSFEVHSLVGIATSNIVLNLDGAN
                     ASGMTFSGGPLNWIVMCPVQVNAYHTAIITLTDANGTVSSTNSFSAFNATNYQWEAED
                     YDYGGGQFFDNPQVDAYANQNGVSGVDFLETDPNIGEQFPYRPSPAMPTTTAGDQARD
                     QFTQASGTDYNIGFFGGGSWCNYTRSYPPGTYNVVGRFAEGAAATEATLSQVTAGFGT
                     ANQTKKFLGTFYVPLGGWSSWAWTSLEDGSGNLVKVTLTGPTNTLQLGGSPVNGQPEV
                     NVNFLMLISTPPYLIATVKVIGTNVTISFPTQTGTTYQVQYKHHLSDANWIPLGSSVS
                     GNNAVQSVNDSVTEASRFYRVQAQ"
     gene            9136..>9626
                     /locus_tag="VG754_06835"
     CDS             9136..>9626
                     /locus_tag="VG754_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWF18966.1"
                     /translation="MTDELAQCFKILDLEPTASPDAVKQAYRELVKIWHPDRFMHDDK
                     LLKRAQAKMKEINVAYETIVEHHACEPPRDFHRASPQPPKPPPSSTASLRTEPAAKAR
                     GYLWGKFFGILLFCQPFFLGSLLSNGDEFFFVTAFFGVLYWPVGYFVIKRKRWAVVLG
                     TLI"
BASE COUNT         2260 a         2510 c         2580 g         2276 t
ORIGIN      
        1 tcatttatct tcatggttgc gtgagcaagt taatccaccg tcactttgcc gaccacatca
       61 ccgggcggac tggctaccaa cgcggatttg ctgatgaaca aagcgaggca gatgtcccct
      121 aaagccctaa agcgaataaa aatttgccaa aacgagcaaa cgagcagtga atttgacttt
      181 tacagctttt gccattgtag gggatccctc aaggtggtaa gatagggact gtagtttgca
      241 agggaatttc gccgcgcaac atcttgccac cctgatttgc cagccgcaaa taccagtcgc
      301 tgttaagagt tattccatcg caatccagcg ccaggaattt gccttgagca ggcagttggg
      361 cggccgttgg cacaagcttg tacatggccg tcccttcgtt gacctcatca aacatcgccc
      421 cgtaaatcat gttgcaaccc gcatgcacat cgtcataaac ctgtctccaa tagaacgagc
      481 cgccattgcg tggaatctgg ttgagtgtgc cgccattttc attgtaccag gaaaacccgg
      541 ggaaaaccac cggcatataa tcaattccgt tcgatttgca ttcggcgaga tccggtatgg
      601 tgatgttggc cgtataactg tctgcacccg cgttgtcgcc aaacctgcca accgcccatg
      661 gacttatgat atcgaacgaa agatagacgg gggtccacgc ggcattggtg tacgaatcac
      721 cctgcagtgt gcgccaatac gtcggcacac cgccaattac ggtgactcct tgggatttga
      781 accaattgat caccgtttgt gcctgctgcg gcgaagccag atggttcccg tcattaaaac
      841 caaaccccca aatagccacc acgggtttgc ctttgtggca aagactccgt gtgctgttcg
      901 taatgtgcat ggtatttacc aagtaaagcc agtcgttggt gatgttgctg atgagggtgt
      961 tggtgggctg tccgctgacg tcatacatga tggcaaacac ccgcccgtag gcttccgctc
     1021 ccgcacggac gttggcagta acctggttac ggaaagaata aaattgcgga ctcgaaagat
     1081 cggccaggaa acgttgtaga aatacgccgt caagttgatt ctcttgcatc cattggaaat
     1141 ggcggaggac ggtttcttgc acggcggacg aatagaattg aaccggactg ccattcgagt
     1201 aggtcatccc cgtgttaaaa agctcgttcg aggtgaactc ggatgtgtct gggagaaaat
     1261 ccgtgttaac gttggcagcc gtcggcgttt ggttgtgaaa ccaatgccac cactgatttg
     1321 gcgccgaacc gtcattcgga catgcgaacc agccttgata gcccatcaac aatttgccct
     1381 tcatggtcgt ggcatcaacc ccatgattca gactgtagaa cgctgtctga ctcgacagag
     1441 gtaaaatgac atcgttagtc ccattggtgg tgatgacagt gttggtcaag gatttccaga
     1501 cgaccggcgc gatgagattg gtggtggact gcaatccata ataaacggaa gaagtcggcc
     1561 aggaaagcac tagattggtt cccgatagtt ggatggaaag ataagctgtg ttgctggagg
     1621 cactactacc ttcagtagcg gtgattgtcg tagtattggc cgttccgttg taattacacg
     1681 caacggtacc agtgccaccg taagcggtaa ttttgctgct tgcgataaaa ccgttcaccg
     1741 aggccagttg attgcccgca ataaccaccg ttccacctcc aacgtccagc acagaaccgc
     1801 tgccttgaat cgagttggta gcccattggg aaagagtcaa tgtgccgccg ttgatgtgca
     1861 tgatgcccct gccgccgcca aagcccagcc cgaaggcacc ggcgaccgaa gcggttccgc
     1921 cattcataat caatgtgccg actgatcccg gattcaagcc gatccacagg tgatagttga
     1981 aggttattga gccgccgttc tcaatgtcca tttcaccggt gttgctatag ccaattgccg
     2041 tccaggcata attgtcatta ttgcctgccg tggtgtcccc ggcagacagg ctgccgccat
     2101 tggtgacaat gagaacgcct cccggtccgc catcaccgat ggttacctgg caaccgccaa
     2161 ccacactgtt cacgatgcac ggcgcggcac cggcattaaa atacgcctta tacccatttg
     2221 tagggacgac gcccccgctc cagttgccat gggtggtcca aatgtcgtcc gaaggctggg
     2281 ttgggttgcc tgccggattc cacgtcgttg cagcaagttg tgccctcgcc aaaacacccg
     2341 cgccaagcag aaaccacacc gcaaatccgc cacaaagttt gcgcaggttt ctcatcacaa
     2401 catgtcaccc acagtgacac accattatac atctgtaatc cacaaaataa aaaaggcttt
     2461 gctctgtatc attgccgtcg tcatgcagat tcgcagtcct gtcgttgtta tcacccgcac
     2521 gcatgatcca gcccagagtc aattgttttg tgctgttcat acatgcgatt gcctggtctc
     2581 gctgctttgc cttgctcaag gctggcaaca acatggccgc aataatggca gcaactcaat
     2641 caacgtaaag cctcgcggga aaggcaataa actgtccgat cgacgagagt gggctgtcat
     2701 tttctcaatt atggaaccgc cccaatcagt ttgccatccc cggaagtggg gataccaatg
     2761 cgccgctcaa ttctgtaact ttcgcacggt ccccaaaact gttaaggcat aacagccaac
     2821 aataatcaaa aaataacacc tccccaatta tgaaacaaat gatgaattcc tctcgaatgt
     2881 tcaaatccct gcttctggcc agcctgctgg ccgggccgat gctggccttc aacgccatgg
     2941 cccagaaccg ggaagacatc aattgggctc cgtcagacat cttcactgac gggaacggca
     3001 accaatacgc ttattgggac gatgctaaca actgggtcgg cggtgtcgtg cccgtcgttt
     3061 atgacacaaa tcaggcgaac actttttaca atgccgcatt tgacagtgac actgttactt
     3121 gtgtggtaac caatgacgct caggtcggcc agctgatggc aggcttcgga ggaggcggaa
     3181 cgctggagat caccaatggt gcccaatttc aggcgggttt ttcgtttgga ggaatttgga
     3241 ctggaatagg ctttgtggct ggacctggga ctttgattgt tgatccgggc agcgacttca
     3301 catgcgcttc acatctctgg gtgggacagg gaaccgctaa ccaaggcacg gtgatcatta
     3361 acggcggcac gcttcatatt ccaaatgggc agttgggcgt gagttggaat ggaatcggcg
     3421 gcaccaacta tattaccatc accaacggcg gtgcattgta catgtcgcaa tggtcttccc
     3481 aaacgcttgg cgcacctggt agtagtagca caaacattgg tataatggac attgaagcca
     3541 actcccaagt ggtgatcaca aacaatcaat taggttatat gaatactttg gagacaaatg
     3601 gtcaactcat cgctttcggc ggtctgggca aaatctctgc ggtctataat ccgtcggcta
     3661 acgtcaccat ccttacaggt cttgcaccct ctggcagcga tacaccagta ttcagccttc
     3721 aaccttcgaa tgtgatcgtt acgttaggcg gcacggccac cttaacagcg acagcctcgc
     3781 ccgccaccgg ctaccaatgg ctgtttaact ccgcgcccct cgcggacggc aatgggattt
     3841 ccggttctaa gactgccact ttaactgttg caaatttcaa tgttgccgag actgggatct
     3901 attccgtggt agctaccaac gcgaatgctg ccagtcagaa cgatcgtaat tatgcctcga
     3961 gccaaggagt ctcagtgact ggcgagtcgt tcaccctata tcccgtcgtc acaattaacg
     4021 gcgttaacgg caacacgtat gtagttcagt ataccacctc gctgacgcag ccagtgactt
     4081 ggacttcgtt ttcgacgaac acagtcggtg ccggcccgct gtatgtcgtt gacaccaatt
     4141 cgccgttgag catgaagcgg ttttatcggg tggtccaacc ataatacgca ggtgttatgg
     4201 ccatgaggga taaggttctt aattccctca tcgcccgtcc caacaagcag gaaactcggc
     4261 ggggccgggt gaattgccgt tggtacgctt ccgaagggtg cttttgccgc ggttgatgca
     4321 aaagtgagga gtcgcagtgt gggaaaatcc gtctcgctcc tcgtctggga gcgggcggat
     4381 gtttcgattg agtggtaagg cgtagtgaac gccacaagag gggaatggaa ctctgtgcat
     4441 cccggccatt ggctccaatt cctgcgaaaa gcgggggtgc ttgcttcatt tgctctggat
     4501 caaaccaaaa ccgaagcagg caaagttatt tcctgcgttg tattcgaagg tcagcagttg
     4561 cagtccggtt tccaaaaaag tgattgtgcc gacttcctaa cgattcgccc gcgttatcct
     4621 caattcactt tatatgtcgc gtgggctgtc agccactgat tatctgtggt tggacctgag
     4681 gtggtgctgt tcgcgttgag aaaaacgtct tgccatttct tgagttgggc atggccgtcg
     4741 gcaaaggaaa aggatgtggc attgccgtga ttcaccgcag gccggtcgtt gagtgcggtc
     4801 ggtttgagca ggaaaaatcc gtcgttgagc gtcacagggt tttcgtccaa aaattgaatg
     4861 caatctgaac tgccaagcac ggagttgaag tcggcgtatt tcgaaaagac cgcccagccg
     4921 ggcaaaatct cgccgggatc atacttgatg gccagcgggg aagttcccac ataacagttc
     4981 gccgaacagg aacgcacccg gagcgctttg gacgcgggat caacgctctt gtctgccggg
     5041 caatgataga caccggcgcc cttggcataa cttccgatcg aaccaaccag ttgcccaact
     5101 ccgggcacat tgtcgccgag cactccaaga aggtagatgt tcgtttcagc gccaagcggc
     5161 gaagcgaccg gattagccgt ggacccaaag ctgccggcga cccaattggg cgcgctcgga
     5221 gcaatgccta tcacactgcc tgaatcagga tgaccctcgt tataggcaat ggtgtcgtta
     5281 ttgtcgcttg catagacgat gctggcgatt tgcaactggc gcatattgtt gacgcacgag
     5341 attccctgcg ccctctgctt ggcttgcgtc agcgcgggca ggagcaaact ggcaaggatg
     5401 gcgatgatcg caatgaccac cagaagttca atcaaggtga atccgtttcc tcgtttcgat
     5461 ttgcgattta aaatatgcgg attcgcgtga gcactcctga acataaaact attcatctct
     5521 attaaattgc ttccggcagc ccccatgtcg gccgccggaa gcattacccg ttttcccaac
     5581 caaaatttat tgcgcttgaa cccggtaaaa gcggctggct tccgtgacag agtcgttcac
     5641 cgattgcacc gcattattcc ctgagacaga actgcccaac ggaatccaat tcgcatccga
     5701 caaatgatgc ttgtattgca cctgataagt cgtgccggtt tgggtcggga acgaaatggt
     5761 gacgtttgtc ccgatcacct tcacagttgc gatcaaatac ggcggcgtgg aaatcaacat
     5821 aaggaagttc acattcactt ccggctggcc gttgactggc gagccgccca attgaagcgt
     5881 gttggttggg ccggtcagcg tcacctttac caaattgccg ctgccgtctt ccaacgatgt
     5941 ccacgcccaa ctggaccatc cgccgagcgg cacatagaat gtccccaaaa acttcttcgt
     6001 ctggttggcg gtgccaaaac cggcagtgac ttgggacagg gtggcttccg tcgccgcggc
     6061 gccttctgca aagcggccga cgacattata agttcccggc ggataagacc gcgtataatt
     6121 acaccacgaa cctccgccga aaaacccgat gttataatcc gttccacttg cttgggtgaa
     6181 ttggtccctg gcctggtcgc cggccgtggt tgtcggcatc gctggggaag gacgataagg
     6241 gaattgttcg ccaatgttgg gatcggtttc cagaaaatcc actccggaaa cgccgttctg
     6301 gttggcgtag gcatccactt gcgggttgtc gaaaaactgc ccgccaccgt aatcgtaatc
     6361 ttccgcttcc cattggtaat tggtggcgtt gaaggcgctg aaactgttcg tggaagaaac
     6421 ggttccattg gcatccgtca acgtgataat tgcggtgtga tacgcattga cctgaaccgg
     6481 gcacatgaca atccagttca gcggtccgcc ggagaaagtc atgccggagg cgttcgcgcc
     6541 atcgaggttt aagacaatgt tgctggtcgc aatcccgacc agtgaatgga cctcaaagct
     6601 caattcattg gtgtactgga acagcgcgga gccgtccggg gtgaagttat ccacgcgcgg
     6661 caggactttg atggtgttat cagcgggcat cagcaggaaa aagttcgcat tgtaattgcc
     6721 gttatccgtg gtgaatctca acgtgttggt gctgccgcca gcgaattgtg ccaggtttcc
     6781 ggcactgtcc ttcaagggaa cccacccata cttttgccat ccgccgcttg ccggaacgga
     6841 gaaaatgcca agcttgctca tggtctggct ggaagtcccc caaccgctgg tcaccaggga
     6901 catgctgcaa ctgttcgagg tggtgccgtt gccatccgcg gcacgcaaga aaatattata
     6961 aatcccggcg ggatatgttc tcgtataatt tccccagttg cctcccacgg aattacctaa
     7021 atcatagtct tgaaaaccgg cgtggctcaa acgcgccacg tcgctggcag tttcggtgct
     7081 caagccgatg cgatcgtatt ggtattgcgc cgggttgctg ttggtgaaat caacgccgct
     7141 gacgccgtcc atgtattggt aggcattcgc ctgcgggtta tcgaagaact ggccgttgcc
     7201 ataatcgaaa tcttccgact cgaatgtgta aaagggaatg atcgtgtcaa aagtcacggt
     7261 ggtgctcgcc ggggtgccgt tggcatctat gacctggatg aacgcggtat agaccatgtt
     7321 gctggtcaac ggaatgctca cgctcaagtt tgtggacgga cctgagacgg tcaatccgtt
     7381 gccgctggag agaagtattg acgagccggt tccggagaga tttgtcgcgc taaactggac
     7441 agtgaggttg ttgctattga cgcccagtgt tgaaacgatg ctgaacgcaa gagcattcgt
     7501 catctggaat tggtaattgc cgtcgggata aatgttggtg atgattgcgt ccgaagcagc
     7561 agaaacattg gtatccacgg gcatcaaaag ataaaaattg gcgttcacgc tgccctgatc
     7621 aatcgtgacg cgaagcgtcg aggcggtgcc gtcgtttggg atcgtgagct gcgcaaagtt
     7681 gccgctcgag tctttcaaag gcacgtaact ataagtctgc cagcttccca atggcggaac
     7741 agcccaggta aaagggccgc tgccggtcag agtgggtgag ccggaataca cggtgagact
     7801 ggcgctgtcc gaactgggac caccgccatc cgagccgcgc atatagacat tatagacgcc
     7861 aggcggatag tgccgcgtgt aattgccata atcaccgcca ttgttccagc caatgtcata
     7921 gtcagtcttg cccgtgccca tgtattgcac acgcggcacg tcgctgttcg actcggatgc
     7981 caggccgggc gggtttggcc gataatcatg attgccgctg ccgtcgtgat gacaatcaat
     8041 cccgtccgtg gcgctcaacg cagcgtaggc gttggtctgg ggattgtcta tgaactggcc
     8101 gctgatgctg ttgctcgtgt aatcgaagtc ttcggcttcg aaagtaaaac cggcgacgat
     8161 ggtgtcaaat gtaacggtgg tgctggtgga accgccgttg ttgtcggtca cttgaatgac
     8221 ggcggtgtaa aagcgattgc tggagagcga gcagcttgcg ctttcattgg tggacggtcc
     8281 cgaaacagcc aaaccgttgc cgggcgacaa agtccttaaa aacggcgagc cgcccgtcaa
     8341 tttggtcgcg gtcagttgca ccgagatgtt tgaaacgccc acggaagaga cggcattaaa
     8401 cgcaagcgtc gaggagggct cgaacaaatg cgcgccgtcg ggataaacat tattgatggc
     8461 cggattggcc tgcgtgcttg ggacgcccag cacgaggcac acaacaccca tgcccatgat
     8521 taacgccgtg agaactttgg tagttgagac gtgaagcgaa tgaattttga agttcatagt
     8581 ttatagagat tgccggggac gatgacaatt tggacgaaag acatttctcg caccatcccc
     8641 gtttgcgggg atgacttggg gaacagagac gacatcaaga cgacacgaat gccgatttgc
     8701 ttccatattt cagggtgacg gggacgttcg cactctgcgt ggaagccgat tttcaaacca
     8761 tccccttttt cggggatgac ctccttatgg ctgcaagaaa gagatcaaaa gcggatgctg
     8821 cgtgggtgta agaatcggca atgttcctca aagcgtccac cacgtcgtaa cacgattgag
     8881 cggccctgcg caaacggccc gatttgtgtc aatagttggg actgatcttt tagatctctt
     8941 tatgataata tttgtatgaa atgcctgtgc gttcggcgaa ctcctcctgt gtcatgcctc
     9001 ggagttttcg caattatttt aatctggcca ccagccgcgc tgaaatgctc gccatggggc
     9061 gagaattccc gttttttctt gagcctcaca cccaactaac ggtagtgttt gtgccgaaaa
     9121 taccgaagcc gacgtatgac cgacgagctt gcccaatgct ttaaaatcct cgacctcgaa
     9181 ccaacggctt cgccggatgc ggtcaaacag gcatatcgcg aactggtgaa gatatggcat
     9241 cctgatcgtt tcatgcacga tgacaagctg ctcaaacgtg cgcaggcgaa gatgaaggag
     9301 atcaatgtgg cgtatgagac catcgttgag caccacgcct gcgagccgcc acgagatttt
     9361 caccgcgcaa gtccacaacc gcccaaacca ccgccttcct caaccgcttc actgcgaaca
     9421 gaaccagcag cgaaagcacg cggttatctt tggggcaagt tttttggaat attgctcttc
     9481 tgtcagcctt tctttcttgg atcgttattg tctaatggcg acgaattctt ttttgtaact
     9541 gcatttttcg gagtgctgta ttggccagtc ggatattttg tgattaagcg gaagcgttgg
     9601 gccgtggtgt tgggaacact gatttt
//
LOCUS       DASZMM010000283         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1763606, whole genome shotgun sequence.
ACCESSION   DASZMM010000283 DASZMM010000000
VERSION     DASZMM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763606"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VG754_06840"
     CDS             <1..195
                     /locus_tag="VG754_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18967.1"
                     /translation="VVFDGHFLRDVKQSFLRESRSATEHAVARACIQAFHQPWKRSAL
                     GSHLESAHFLRIAFPAFTAF"
     gene            361..1803
                     /locus_tag="VG754_06845"
     CDS             361..1803
                     /locus_tag="VG754_06845"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HWF18968.1"
                     /translation="MSNVVSNLRPEPDPLLVDIADYVCAHAIQSKEAYQTARYCLVDT
                     LGCGLLALNFPACMKLLGPVVPGTTVTGGSRVPGTRFELDPVAAAFNIGCAIRWLDFN
                     DTWLAAEWGHPSDNLGAILALTDFLSRRQRAEGKKPFTIRDVLTAMIKAHEIQGMLAL
                     ENSFNRVGLDHVILVRIASTAVATQLLGGTREQIINAVSNAWLDGGALRTYRQAPNTG
                     SRKSWAAGDATSRGVQHALMALKGEMGYPSALSAKTWGFQDVLFRGQPVKLSNRLTSY
                     VMENVLFKISFPAEFHAQTAVEAAVQLHPQVKDRLEDIEQIVLTTHESAIRIIDKSGP
                     LHNPADRDHCLQYMVAIGLLFGRLDASHYEDATAKDLRVDALRAKMAVKEDKRYSREY
                     LEPDKRSIANAVQIFFKDRKSTPKIEVEYPLGHRRRRAEGIPLLMNKFKANLVSHLPA
                     HRVENIWNICNDPPRLEATPVHEFVEHFVI"
     gene            2066..2869
                     /locus_tag="VG754_06850"
     CDS             2066..2869
                     /locus_tag="VG754_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF18969.1"
                     /translation="MKTNLSGKWVIITGASSGFGAAAARAFGAEGAKLLIGARRLDRL
                     ETVADEAKKAGAAEAHFHTLDVSKTPSVNEFVAWCKEVMTRNSEPGTRNQIDVLINNA
                     GGALGMDTVAEGKDEDWETMLQTNVLGVLRMTRAVLPLMLNNPGSSILNIGSLAARVA
                     YEGGAAYCAAKAGELQITRTLRLELSGTGIRVSSIDPGLAKTDFSVVRFKGDADKAEK
                     PYEGTIPLTAEDIAETLVWVASRPPHVNIDEMLIKPTDQASINKVYRRK"
BASE COUNT          666 a          837 c          787 g          635 t
ORIGIN      
        1 gttgttttcg atggacattt ccttcgcgat gtgaaacaat ctttcttgcg tgaatcaagg
       61 agcgcaacag agcacgccgt cgcgcgtgca tgcatccagg cttttcatca gccatggaaa
      121 aggtctgcac tcggatcgca ccttgaatcc gcacattttc tgcggatcgc gttcccggct
      181 tttaccgcct tttaacgcct gttcacgcct atcttgcagg ggggaggggg gacgccggca
      241 ccaaaatttt tctttcatga acattgtgac cctttaacca tgtaacgttt tcccgcgcac
      301 atccatcccc gcttgacctc acggcgcgct tgccctttca tcggcggacg aatgagtcgt
      361 atgagtaatg ttgtcagtaa tttgcgtccc gaaccggacc cgttgttggt ggacatcgcc
      421 gattatgtct gcgcccacgc catccaaagc aaggaagcct accaaaccgc gcgctattgt
      481 ctcgtggaca cgctcggctg cggtctgctg gcgttgaatt ttcccgcgtg catgaaattg
      541 ctcggcccgg tggttccggg aacaaccgtg accggcggca gccgcgtgcc gggaacgcgt
      601 tttgaactgg atcctgtcgc cgctgcgttc aacatcggct gcgccatccg ctggcttgat
      661 tttaacgaca cctggctcgc cgccgaatgg ggccatccat cggacaatct cggcgcaatt
      721 cttgccttga cggatttcct cagccgccgt caacgcgccg aaggcaaaaa accgttcacc
      781 atccgcgacg tgctgactgc gatgatcaag gcgcacgaaa tccagggaat gctcgccttg
      841 gaaaacagtt tcaaccgcgt cggcctcgac cacgtcattc ttgtgcgcat cgcttccacg
      901 gcggttgcga cgcaattgct cggaggcact cgcgagcaga ttatcaacgc ggtttccaac
      961 gcgtggctcg atggcggggc gttgcggact tatcgccagg cgccaaacac cggctcgcga
     1021 aaatcctggg ctgcgggcga cgccaccagt cgcggcgtgc aacacgcgtt gatggcgctt
     1081 aagggcgaga tgggttatcc ctccgcgctt tcagccaaga cctggggttt tcaagatgtg
     1141 ctgttccgcg gccagccggt caagctttcc aaccggctca cttcttacgt gatggaaaat
     1201 gttcttttca aaatttcctt tcccgccgag ttccacgcgc aaaccgccgt cgaggccgcc
     1261 gtccaacttc atccgcaagt caaggaccgg ctcgaagaca tcgaacaaat cgtcctcacc
     1321 acgcacgaat cagccatccg catcattgac aagagcggcc cgcttcataa tcccgccgac
     1381 cgcgatcatt gcctgcaata catggttgca atcggattgc tgttcggacg tcttgacgcg
     1441 agccattacg aagacgccac cgcgaaagat ttgcgcgtgg acgccttgcg tgcgaagatg
     1501 gcggtgaagg aagacaagcg ttacagccgc gaatatctgg aaccggacaa gcgctccatc
     1561 gcgaatgccg tgcagatttt tttcaaggac cgcaagtcca cgcccaaaat cgaagtggaa
     1621 tatccgctcg ggcatcgccg tcgccgcgcc gaaggcattc ctttgttgat gaacaaattc
     1681 aaggcaaacc tcgtatcgca ccttccggcg catcgcgtgg aaaatatttg gaatatttgc
     1741 aacgacccgc cgcgattgga agccacgccg gtccatgaat ttgtggaaca ttttgtgatt
     1801 taaaattgtg aaaaaggaat ttagtcgaac gctcgccctc acccctgccc tatcgccctg
     1861 ggagagggag aacgcagcca acgtcttcga ctgtttgacc gtcgcggttt tattcccaac
     1921 ttctgaagtt tccaggagca gcaaaagcac aaacattttt ttcataccgt aaacgccaaa
     1981 ccgcttcacc ctctcctggg gaagagggcc gggttgaggg caaacgaatc actgatttat
     2041 ttaaattcag aagaatgcga atagtatgaa aacaaacctt tcaggcaaat gggtcatcat
     2101 caccggcgcg tcgagcggat tcggcgctgc ggccgcgcgc gcttttggcg ctgagggcgc
     2161 aaaacttttg atcggggcgc gaagattgga ccgccttgag accgtcgccg acgaagccaa
     2221 gaaagccggc gctgctgaag cgcattttca cacgctcgac gtttccaaaa ctccgagcgt
     2281 gaatgaattc gtcgcctggt gcaaagaagt aatgacccgg aactcggaac ccggaactcg
     2341 gaaccaaata gacgttctca tcaacaatgc cggtggcgcg ctcggtatgg acaccgtggc
     2401 tgaaggcaag gatgaagatt gggaaacgat gctgcaaacg aacgttctcg gcgtgttgcg
     2461 catgacgcgc gccgtgctac cgttgatgtt gaacaatccg ggcagcagca ttctcaatat
     2521 tggttcactc gcagcgcggg tggcttacga aggcggcgcg gcttattgcg cggcgaaagc
     2581 cggcgagttg cagatcacgc gcacgttgcg gcttgaattg agcggcacag gcatccgtgt
     2641 ttcttcgatt gatcccgggc ttgccaagac cgatttttca gttgtgcgtt tcaaaggcga
     2701 tgccgacaag gccgaaaagc cttatgaagg caccattcct ttgaccgccg aagacatcgc
     2761 cgagactctt gtctgggtcg ccagccgacc accgcatgtg aacattgacg aaatgctcat
     2821 caaaccgacc gaccaggcgt cgataaacaa ggtgtatcgg agaaaatgag ttacatcgtt
     2881 gaatggttac aaagttacaa agagcgccac catgtaacca tgcaa
//
LOCUS       DASZMM010000284         7871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_104600, whole genome shotgun sequence.
ACCESSION   DASZMM010000284 DASZMM010000000
VERSION     DASZMM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104600"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VG754_06855"
     CDS             complement(<1..298)
                     /locus_tag="VG754_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18970.1"
                     /translation="MTFDRARARSRQAFTLIEVMVAVCVIIVAVTSIFASMSMGFSMT
                     ATSRENLRATQIMLDKVEGIRLYNWSQVTNSAYLIPAFTNWFYETNNIGDANATG"
     gene            complement(508..1308)
                     /locus_tag="VG754_06860"
     CDS             complement(508..1308)
                     /locus_tag="VG754_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18971.1"
                     /translation="MNYTKTIRIVVGVVLAALAGGHVQAAETLPILKAGPQTFTNVVI
                     VDVSDTQLFFKYAGGMSSVRLKDLEPTLQKTYGYDAAKEAAIESAPPVSVVTPVSDTP
                     VETRKTSVPDGMVSRDFRLEDGRRLRLCFPKVWTAPDDGSSSLLKFQHPYEEGFSVLV
                     SVMPKKNVFANMETQDALAVPGNQELRYSLEKSLTLEPINGTEAKGHYFVLTRKSPGQ
                     KQTPCESQGLVRFGNLLVYFCIECKDWKGKEPQYALEMIQTAQLVAKP"
     gene            1419..2975
                     /gene="xylB"
                     /locus_tag="VG754_06865"
     CDS             1419..2975
                     /gene="xylB"
                     /locus_tag="VG754_06865"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HWF18972.1"
                     /translation="MPARTLLIGIDSGTQSTKVLVVDAQNGKVLASAQQVYGLIPNLP
                     PGAKEQAPETWRAATESAIRQALRQAKALTAEVVAIGVSGQQHGFVPLDAKGEVIRPA
                     KLWCDTSTTAECAEITEKLGGQKPTIRALGNTVLPGFTAPKILWLKKHEPKNFERLAT
                     VLLPHDYLNFWLTGERVMEYGDASGTALLDVRKRKWSQAALEAIDPALADKLPPLIAS
                     DQPAGKLQKSTAKMLDLNTDIVVSAGGGDNMMGAIGTGNTTAGVITASFGTSGTIYAC
                     AEKPVVDPQGEIAAFCDSTNRWLPLLCTMNVTTATEMVRIANAWSHERFSTTVARVPV
                     GSGGLVLLPYLEGERTPNVPAGTGVLFGVNQRTYTPDFLARAAMEGVTLGMNFGLRRL
                     KELGVNPTQIRATGGGAKSKAWRQIMADIFNTEVVTLKVGEGAAYGAALQAFWCWRKQ
                     KGEKLGIKEITDRFIELNSEETTQPISKNVAIYQELQALQDELSSSLRDVFEKHRALV
                     IRMENQKQGG"
     gene            2976..4004
                     /locus_tag="VG754_06870"
     CDS             2976..4004
                     /locus_tag="VG754_06870"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWF18973.1"
                     /translation="MFILLPVGMDYRARRYPVVTFTIMGICTALYLVDLACKLGGDAQ
                     AVWDWDFEHLWLIPSESHWWTYITSMFVHQGFFHLFGNMIYLFLFGACVEDMIGRLHF
                     SIFYLVCGIGSSLAYIMFSPQHFASDIPEGGASGAISACIGGYLLLRAKAHIQFKWIV
                     IFFFRFFQGDFTMPAWIAISFWFLKDFGMMLLSAALDAHGGGVAFGAHVGGTLLGAGF
                     IALEKIRLKKMPQEIEEEEVAIKPVQIVRKAPAAWAAPRPGQTRTISLHPAASEPPTP
                     APAAVPATSEPPTIHLSWDGAQSGPFTLSQVQQMFAAGEIPTTAYYWQEGMSDWRSAE
                     ELREPGVG"
     gene            complement(4042..4680)
                     /locus_tag="VG754_06875"
     CDS             complement(4042..4680)
                     /locus_tag="VG754_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HWF18974.1"
                     /translation="MATEERVLCFKRNLLEELGVFQGISMDVEKYLPVVTATSNVLYL
                     NRSDAEQDRRHKQLIPYVLILCGDKILRYRRGKGGGEARLHGLFSVGIGGHISEEDNG
                     LFANASGYAEGMRRELLEEVAIDEVNETAVGLINDDSTEVGYVHFGVVHVMQVNSEEI
                     AGRCKGIVAPEFIPLSEAMKDPSGYESWSRFCLENLPLLLAKAADFGTAPAS"
     gene            5353..5919
                     /locus_tag="VG754_06880"
     CDS             5353..5919
                     /locus_tag="VG754_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HWF18975.1"
                     /translation="MKKVFTPILLGVLAVALPSLADDKWNISKLDASKLPPSASKTSI
                     TFEQDIKPMFEKSCVRCHGDNQQKGGFRVDSLVAVLKGGKAGKMIVPGDSTKSLLVLA
                     VSQQDNGTAMPPKRKRPPGGPGGGPPDASSPPGGSGRGPGGPDGQGVPGGGPFGGTPG
                     PGRGGPPAKALTTEQVALVRAWIDQGAK"
     gene            complement(5939..7072)
                     /locus_tag="VG754_06885"
     CDS             complement(5939..7072)
                     /locus_tag="VG754_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18976.1"
                     /translation="MAKFTWTAKDASGNQVVRVISAANAEEGKAILLAEGCTDLALRE
                     DEIHHIAQEHFSKPMVFLGEEIKVTADQRIKQMDKPNRSAFGRIVDSVIQDKGLYGLL
                     LLLIAFEFYRQQYIKAGGFALALLCWPVFRIWLNLPSHYYRQLNKAKDWHRWQEVMTL
                     LEKTVKISKTHPVKIPATELARIRAQALVGLGHLEEGVAAMQLLENRPGNPSWLHKAH
                     MASLYDIAKQHDTAIEYTRLGIAENPSPVLYADLANRLLRYKRDTVQAREALAIVDKA
                     TMSETAKPFVIRCHGILAYLEGDHPAAKKDLETAIEMVEKAHKRPFRDGALAVSRAYL
                     CCTLARLGDTDGAKKMFALAKDYLVATDEKELIAECNRAIGEA"
     gene            complement(7354..>7871)
                     /locus_tag="VG754_06890"
     CDS             complement(7354..>7871)
                     /locus_tag="VG754_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18977.1"
                     /translation="IRVHNVAVKDAGTFFGAVLVVAGLMGTLIGGFAATAWQKRNPAG
                     YAWTLALSVLLAVPFVVLAFVIKDTNVSMSCLALSMFLLFLCTGPVNTLIIETVPVNL
                     RASAMALSIFMIHLFGDVWSSEIVGRLADYWNDLSKAVLILPAALFVGGLLWLWLALT
                     ERKHRTLNIEH"
BASE COUNT         1812 a         2303 c         2014 g         1742 t
ORIGIN      
        1 tgcccgtcgc gttggcatcg ccgatgttgt tggtttcgta aaaccagttc gtaaaagccg
       61 ggatgaggta ggccgaattg gtcacttgag accagttgta gaggcggatg ccttccactt
      121 tgtccagcat gatttgtgtc gcgcgaaggt tttcacggga ggttgccgtc atcgaaaagc
      181 ccatcgacat cgaagcgaat atcgacgtca ccgcaacgat aatcacgcat accgcgacca
      241 tcacctcgat cagcgtgaag gcttgccggc tgcgggcgcg ggcacggtca aatgtcatct
      301 caaagttttt cactcaattt cctcaatttg tccggcccat aggaccggag ccaaaaactg
      361 tccctttcgg gaagcaactc ggttgccaag tcaattcttg gggcatttgc tgcctatttt
      421 tccttcaaac ccacgtaaaa ctacggaagt gtcttagata aaagtacgga ttgtccaaaa
      481 ttgaggcagg gctgcccgtt ccctcggtca aggtttggca accaattgcg ccgtctgaat
      541 catctcaagc gcgtattgcg gctctttccc cttccagtct ttgcactcga tgcaaaaata
      601 aaccagcagg ttgccaaatc tcaccaaccc ttgtgattcg cacggggtct gcttttgtcc
      661 cggtgatttg cgggtcaaga cgaagtaatg gcctttcgct tcggtcccgt tgatcggttc
      721 caaagtcagg ctcttttcca acgaatatct caactcctga tttccaggaa cggccagcgc
      781 atcctgggtt tccatgtttg caaaaacatt ctttttgggc atcaccgaaa ccaaaactga
      841 aaagccttcc tcatacggat gctgaaactt taacagagaa ctggatccat cgtcgggggc
      901 cgtccaaacc ttcggaaaac aaagccgcag gcgccttccg tcctcaaggc gaaaatccct
      961 tgaaaccatg ccgtccggca cggaagtttt gcgggtctcc accggcgtat cgctcacggg
     1021 agtcaccacc gatacgggcg gtgcgctttc aatcgcggct tcttttgccg catcgtaacc
     1081 gtaagttttt tgaagggttg gttccagatc cttgagcctt acgctgctca tgccgcccgc
     1141 gtatttgaag aacaactgcg tgtcgctcac gtccacgatc acgacgttgg tgaaagtttg
     1201 tggaccggcc tttaagatcg gcagtgtttc tgccgcctgc acatgtcccc cggcgagtgc
     1261 ggcaaggaca accccgacaa caatgcgaat cgtttttgta tagttcatgg gcatttacac
     1321 gcgggaaacc cgcaattcac cctctaatgg cactttttgt gccaagcggc gggatattat
     1381 tgcttcacac cgtcccggcg gtttggtttg gtataggaat gcctgcaaga acacttttga
     1441 ttgggattga tagcggcacg caatcgacca aggtcctggt tgtggatgcg caaaacggca
     1501 aagtcctcgc ttccgcgcaa caagtctatg gcctcatccc caatctaccg cccggcgcaa
     1561 aggaacaagc gccggaaacc tggcgcgccg ccacggaaag cgccatccgc caggcgttgc
     1621 gtcaggcaaa ggcattaacg gcggaagtgg tggccatcgg cgtcagcgga cagcaacatg
     1681 gttttgtgcc gctcgatgcc aaaggcgaag tcatccgccc ggcgaaactt tggtgcgaca
     1741 cttccacaac ggccgaatgc gcggagatca ctgaaaaact tggcggccaa aaaccaacca
     1801 tccgcgcgtt gggcaacacc gtgcttcccg gtttcaccgc gccgaaaatt ctctggctca
     1861 aaaaacatga accgaaaaat ttcgagcgcc ttgcgaccgt cttgttgccg catgattacc
     1921 tgaatttttg gctcaccggc gagcgcgtca tggaatacgg cgacgccagc ggcaccgccc
     1981 tgctcgacgt gcgcaagcgc aaatggtcgc aagccgcgct ggaagcgatt gatcccgcgc
     2041 ttgcggataa attgccgcca ttgattgcca gcgaccaacc cgccggcaaa ttgcagaaat
     2101 cgaccgccaa gatgctcgac ctgaacacgg acatcgtcgt gagcgcgggc ggcggcgaca
     2161 acatgatggg cgccatcggc accggcaaca ccaccgcggg cgtcatcacc gccagcttcg
     2221 gcacgagcgg aaccatctac gcctgcgcgg aaaaaccggt tgtggacccg caaggcgaaa
     2281 tcgccgcctt ctgcgattcg accaatcgct ggctgccgct cttgtgcacg atgaacgtga
     2341 ccaccgcgac ggaaatggtg cgcattgcga acgcctggtc gcatgaaaga ttttccacca
     2401 ccgtcgcgcg cgttcccgtt ggctccggcg gactcgtttt gctgccgtat ctcgaaggcg
     2461 aacgcacgcc gaacgtccca gccgggaccg gcgttctctt cggcgtgaac cagcgcactt
     2521 acacgcccga ttttctcgcg cgcgccgcga tggaaggcgt gacgctcggg atgaatttcg
     2581 gtttgcggcg cttgaaggag cttggcgtca acccgaccca gattcgcgcg accggcggcg
     2641 gcgcaaaatc aaaggcatgg cggcaaatca tggccgacat tttcaacacg gaagtcgtca
     2701 cgctcaaggt gggcgaaggc gcggcttacg gcgcggcatt gcaggcgttc tggtgctggc
     2761 gcaagcagaa aggcgaaaag cttgggatca aggaaatcac cgaccgcttc atcgaattga
     2821 attccgagga aaccacgcaa ccgatttcga aaaacgtcgc catttaccag gaattgcaag
     2881 ccttgcagga cgaattgtca tccagcctgc gcgacgtgtt tgagaagcat cgcgctttgg
     2941 tcattcggat ggaaaaccaa aaacaaggag gttaaatgtt catcctattg cccgtgggaa
     3001 tggattaccg cgctcggcgg tatccagtgg tgacgttcac gataatggga atttgcaccg
     3061 ccctttatct tgtggacctt gcgtgcaagc tcgggggcga cgcgcaggcg gtttgggatt
     3121 gggacttcga acatctctgg ctcattccaa gcgaatcgca ttggtggact tacatcactt
     3181 ccatgtttgt tcaccaaggt tttttccacc tcttcggcaa catgatttac ctgttcctgt
     3241 tcggcgcgtg cgtcgaggac atgatcggca ggttgcattt cagcattttt tatctggttt
     3301 gcggcatcgg ctcatccctc gcctacatca tgttttcgcc gcaacatttt gcgtcggaca
     3361 ttccggaagg cggcgcgtcc ggcgcgatct ccgcctgcat cggcggctat ctcctgctgc
     3421 gcgcaaaagc gcatatacag ttcaaatgga tcgtcatttt cttcttccgc ttttttcagg
     3481 gcgatttcac catgcccgcc tggatagcga tttcgttttg gttcctcaaa gatttcggca
     3541 tgatgctctt atcggccgca ttggacgcac acggcggcgg tgtggctttt ggcgcgcacg
     3601 tcggcggaac gttgctcggg gcgggcttca ttgccctgga aaaaataagg ctcaagaaga
     3661 tgccgcagga aatcgaagaa gaagaagtcg cgatcaagcc tgtgcaaatc gtcagaaaag
     3721 cgcccgccgc atgggccgct ccgcgtcccg ggcaaaccag aaccatatcg ctgcaccctg
     3781 ccgcaagcga gcctccaact cctgcgcccg ctgcggttcc ggcaacgtcc gaaccgccga
     3841 ccatccatct ttcgtgggac ggcgcgcaat cgggaccctt cacgctttcg caagttcagc
     3901 aaatgtttgc cgctggagaa attcccacaa ccgcgtatta ctggcaggaa ggcatgagcg
     3961 actggcgcag cgcggaagaa ttacgggaac cgggtgtcgg gtgaatctgg aacgcaactc
     4021 aagcgcacat ttatccaacc atcaggaagc tggcgctgtc ccgaaatccg ccgctttcgc
     4081 caacagcagc ggaaggtttt ccaggcaaaa ccgcgaccat gattcgtagc cggacgggtc
     4141 tttcatcgct tcggaaaggg ggatgaattc aggcgcaaca attcctttgc aacgcccggc
     4201 gatctcctcg ctgttcacct gcatcacgtg caccacgccg aaatgcacat aacccacctc
     4261 cgtgctgtcg tcattgatca acccgaccgc agtctcgttc acttcatcga tggcaacttc
     4321 ctccagcaac tcgcgccgca tcccttccgc gtaacccgaa gcattcgcga acaacccgtt
     4381 gtcctcctcc gaaatgtgcc cgccaatccc gacggaaaac aatccatgca accgcgcctc
     4441 accgccgccc ttgccgcggc gataacgcaa aattttgtcg ccgcaaagaa tcagcacgta
     4501 aggaatgagc tgcttgtgac gccggtcctg ctcggcatcg ctgcgattca ggtacagcac
     4561 gtttgacgtc gccgtcacca ccggcagata tttttcaaca tccatgctga tgccctggaa
     4621 cacgcccaat tcttccaaga gattccgttt aaaacaaagc actcgttctt ccgtcgccat
     4681 gcgtcaatta cctttcgtgt tttaatataa ccgcagaaaa gcgcagataa acccagatac
     4741 gaccatcgaa tgcgcaacac aaccaaccca ccacctcgca tctcgatgcc tgtttttatc
     4801 tgcgctcatc tgcggttgaa gcagttgttc tgttcaatcg agattcagga tattgttacg
     4861 cgtaattgca aggcacgacg agaaaaacat attcgcctct gttccctgac tattcgccgg
     4921 ttgttgacct tatattccac acttattcac atgctgaaat gggcgcgtca agccagccgg
     4981 gacctgcgat tttattggca aaaccagata aacggcagaa aatgagatta agagttttta
     5041 ttaggaaatc aagaaatcag gaacaaaact cttgaggaaa gatcgtcttt tcctagtttc
     5101 ctgatttcct aattcaattt tcccaccgga tggctctgaa atccatcaaa cacttaaaaa
     5161 tggcaggccg tatcaactgg atcccaataa aaagttcgtc cggcaggctg aaccaacgaa
     5221 gaaggctttg aatgaaaaaa aatcttgtca aaccacatgc ctgaaaattc gtgtgttcaa
     5281 caaagacttg tgtcagcagg ccctgtcttg ctaccgtttc aacccaagaa gaattggaat
     5341 tcacaatcat ctatgaaaaa agttttcacg cccatcctgc tcggtgtctt ggccgtcgcg
     5401 ttgccgtcgc tcgccgatga caaatggaac atcagcaaac tcgatgcctc caaacttcct
     5461 ccatccgcct caaagacctc catcacgttc gaacaggaca tcaagccgat gttcgaaaag
     5521 tcatgcgtcc gttgccatgg tgacaatcag caaaagggcg gtttccgcgt tgattcgttg
     5581 gtcgcagtgt tgaaaggcgg caaagccggc aagatgattg tccccggcga cagcaccaag
     5641 agcctgctcg ttctggcggt ttcccagcag gacaacggaa ctgccatgcc gcccaagcgc
     5701 aagcgtcctc ccggcggacc tggtggcggc cctcccgatg ccagttcgcc gcccggcggt
     5761 tcaggtcgcg gccccggcgg gccggatgga caaggtgtcc ccggcggcgg tcccttcggc
     5821 ggaacgcccg gtcccggtcg cggcggtcct ccggctaagg cgttgacaac ggagcaagtc
     5881 gcccttgtgc gcgcctggat tgatcaaggc gcgaagtaaa aaatttggtc aacgaaactt
     5941 aagcttcgcc gattgcacga ttgcattcag cgattaattc tttttcatcc gtcgcgacca
     6001 gataatcttt cgcaagcgcg aacattttct tcgcgccatc cgtgtctccc aatcgcgcaa
     6061 gcgtgcagca aagatatgcg cgggacaccg caagcgcacc gtcgcgaaaa ggtcttttat
     6121 gcgccttctc caccatttcg atggcggtct ccaaatcttt tttagcggcg ggatgatcgc
     6181 cctcaaggta agccaagatg ccgtggcaac ggatgacgaa cggcttggca gtctcactca
     6241 tcgtcgcctt gtccacgatg gccagcgctt cgcgggcttg aaccgtgtcg cgcttgtaac
     6301 gcagaaggcg gttcgccaaa tccgcgtaaa gcaccggcga aggattttcc gcgatgccca
     6361 agcgggtgta ctcgatggcc gtgtcgtgct gttttgcgat gtcatacagg cttgccatgt
     6421 gcgccttgtg cagccacgaa ggatttcccg gccggttctc aagtagttgc atcgcggcca
     6481 ctccttcttc caggtgtccc aatccaacga gtgcttgcgc ccggatgcgc gcgagttccg
     6541 tcgcggggat tttgaccggg tgggtcttgg aaatctttac cgtcttttcc aacaacgtca
     6601 tcacttcctg ccagcgatgc cagtctttgg ccttgttgag ctgcctgtaa taatgagacg
     6661 gcaggttcag ccaaatacga aaaaccggcc agcaaagcag cgcgagcgcg aagccgcccg
     6721 ctttgatgta ctgctgccta taaaactcaa aagcaatgag cagcagcagc aatccgtata
     6781 atcccttgtc ctggatcacg ctgtccacga tcctgccgaa tgcggaccgg ttcggtttat
     6841 ccatctgctt gatgcgttgg tcagcagtga cttttatctc ctcgcccaaa aagaccattg
     6901 gcttgctgaa atgctcctgg gcgatgtgat ggatttcatc ctcgcgcaag gcgaggtccg
     6961 tgcagccctc agccaacagg atggctttgc cttcttcggc gttggcggct gaaattaccc
     7021 gcacgacttg atttcccgat gcatccttcg ctgtccacgt aaatttcgcc ataaggcaac
     7081 cctctgaaat gtttttggtt tcccaaaaat gctatgaaaa aacgccctct ccggcaacag
     7141 caatccgacc aacgctgaac atccaacacg aacattgaac tccaaaaacg cgcacgcctc
     7201 acaagtcatc tcataaattg tggagcgcgg ctgtgcgcga agcgccagcc gcagcaaacc
     7261 atcgaaatcc tgagtcttcc aagctgcgac tggtcgtgcg gacacagtcg cgctccgttt
     7321 tttgttttat gagatgactt gtaaattgga tgttcagtgt tcgatgttga gcgttcgatg
     7381 ttttctttca gtcagcgcca gccagagcca cagcaaacct cccacgaaaa gcgccgccgg
     7441 caaaatcaaa accgccttgc tcaaatcatt ccaataatcc gcgagtcgcc caacaatttc
     7501 ggaagaccag acatcgccaa acaggtggat catgaaaatt gaaagcgcca tcgcgctggc
     7561 gcgcaggttg accggcacgg tttcgatgat gagcgtgttc accggccctg tgcagaggaa
     7621 cagcaggaac atcgaaagcg ccaggcacga catcgaaaca ttcgtgtcct tgatgacgaa
     7681 tgccagcacc acaaacggca ccgccagcaa aaccgacaat gccagcgtcc acgcgtatcc
     7741 cgctggattg cgtttttgcc acgccgtcgc agcgaaaccg ccgatcaatg ttcccatcag
     7801 tccggcaacc accaacaccg ctccaaaaaa tgttccggca tcttttacag ccacgttatg
     7861 cacacgtatc a
//
LOCUS       DASZMM010000285         5056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4784078, whole genome shotgun sequence.
ACCESSION   DASZMM010000285 DASZMM010000000
VERSION     DASZMM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4784078"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VG754_06895"
     CDS             <1..241
                     /locus_tag="VG754_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18978.1"
                     /translation="IMLTGKFLYATRVSMTYALLRLEEGPDTHYNLNLVDPSNGSLIW
                     NYHQGNHPVMKTEVQKNWILLQMDDEVVVLKFFTF"
     gene            complement(313..1605)
                     /gene="clpX"
                     /locus_tag="VG754_06900"
     CDS             complement(313..1605)
                     /gene="clpX"
                     /locus_tag="VG754_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWF18979.1"
                     /translation="MARPTNITLCSFCGKSHAEVKKLIAGPGVYICDNCVILCKSVLD
                     KELSAQPKRHKPKLNIPKPAEIKRQLDRQCVGQEHAKKTLAVAVHNHYKRILQEPPPP
                     DGEPDDTLPLDRHAEVEIEKSNILMLGPTGSGKTLLARSLAQILDVPFAIADATTLTE
                     AGYVGEDVENIILRLLQNADYDVKRAQRGIVYIDEIDKIARKTENVSITRDVSGEGVQ
                     QALLKILEGTVCNVPPQGGRKHPQQEYIRVDTSNILFICGGAFVGLERIIQRRLGKHV
                     MGFNAVEQGHRAASVDRVQILQYIEPEDLLSFGFIPEFIGRLPMVTVLSELTEDQLVS
                     ILTDPKNALVKQFAKLLSMEGVELEFSPDALRMLAMLALKKGTGARALRGLLERVMLD
                     LMYEVPNSDDILTVKITSSVVMGESKPIIRRKQDQAAA"
     gene            complement(1612..2259)
                     /gene="clpP"
                     /locus_tag="VG754_06905"
     CDS             complement(1612..2259)
                     /gene="clpP"
                     /locus_tag="VG754_06905"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HWF18980.1"
                     /translation="MTMDNFLVPMVVEQTGRGERGYDIYSRLLVDRIVFLGTPVDDMV
                     ANLIIAQLLFLQMNDPKKDIHLYINSPGGSVTAGLAIYDTMQFLTCDVNTYCIGQAAS
                     MGAVLLCGGTKGKRYALPNANVMIHQVLGGAEGPASDVEIRVKYMLKLKQRLNGIISK
                     HTGKSIEQVEKDCDRDNFMSAEEAKGYGLVDEVVQSRKEISTLAEHATSLADKKE"
     gene            2425..4488
                     /locus_tag="VG754_06910"
     CDS             2425..4488
                     /locus_tag="VG754_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18981.1"
                     /translation="MKAELNSLDEHRQVRLICIALALGTLFLYCPVLGHDFITFDDWA
                     YVLANPHVRDGLSLSGISWAFAGGSYSGNWHPLTWISHMVDCQLYGLDHPGGHHFTNV
                     LFHIANTLLLFVLLKRMTGALWRSAAVAALFAWHPVHVESVAWIAERKDVLSAFFWLL
                     TMLAYVRYAAESQAKGRRTKTYYTMALIGFALGLMSKPMVVTLPFVLLLMDYWPLKRG
                     MDPQTPGDLQWAQWKPLVREKLPFIALSFISCVVTFISQKEGGAVSTLAQVPFWIRLF
                     NTQISYVLYLGKIIWPTKLAILYPLEPLPGWMWIASCAVLLIISSLALIMMRRCPWLF
                     TGWFWFLGTLVPVIGLVQVGRQLIADRYTYLPAVGIFIAVVWGCGEFLARVPRLRPMA
                     IVAGAMGLCICMGITENQLGYWKDSVTLFTHALAVARPNTTALNCLALGLERQHKMEE
                     AMAKYKIALQIEPQSFATFHLMGLNLASRGNYQDAVECYNHALSFKDDPTIRYNLGNA
                     LAAQGKYAEAEAQYTQAIRADPDSADLHNNLGAMLARRGLLDEAMRHFQAALRLQPNY
                     PEAQDQLAALFQKQGRLDLARIHYAEAIRLKPDFAHAQLKLGYVMAQQGDIEAAIPHF
                     QKAITLESTNADAYFDLGAAYAALENWRPAVDAFSNAAQLKPGSAEFKGRLAEARAKA
                     SGAKK"
     gene            complement(4517..>5056)
                     /locus_tag="VG754_06915"
     CDS             complement(4517..>5056)
                     /locus_tag="VG754_06915"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWF18982.1"
                     /translation="CLSVLTDEQFFQGSLEYLKQIRQAVKLPLLRKDFIIDERQILEA
                     IEWGADAILLIVAILTDAQLKNFHALATEAGLTALVEVHDEAELNRALAAGAKVIGVN
                     NRDLKSFKVDLATTERLAAKLRGAPNGSEKLLVAESGIHTRADVERLAKCGAQVILVG
                     ESLMKHRDIGGKIAELLGG"
BASE COUNT         1076 a         1416 c         1359 g         1205 t
ORIGIN      
        1 aatcatgctc accggaaaat tcctgtatgc gacgcgggtt tcaatgacat acgcgctgct
       61 gcgtctcgag gaagggcctg acacacatta caacttgaac ctggtcgatc cttccaacgg
      121 cagcctgatt tggaattatc atcaaggcaa ccaccccgtc atgaaaaccg aagtgcagaa
      181 aaactggata ctgctgcaaa tggatgacga agtggtggtg ttgaaatttt ttacgttttg
      241 accgcggaca ttagttccgc ggttcgctct ttgaaataaa aaaggctgct cgatcttctc
      301 ctgtgaaaga gattaagccg ccgcctgatc ttgcttgcgg cggatgatgg gcttgctttc
      361 gcccatgacc acgctactgg tgatcttgac ggtgagaatg tcatcggagt tcggcacttc
      421 gtacatcaga tcgagcatca cgcgttcgag caagccgcgc aacgcccggg cgccggtgcc
      481 ttttttcaac gccagcatgg cgagcatgcg caatgcatct ggcgaaaatt ccagttccac
      541 gccttccatc gaaaggagct tggcgaactg tttcaccagc gcgttcttcg ggtcagtcaa
      601 gatggaaacc aactggtctt ccgtcaactc ggacaacact gtgaccatcg gcaatcgccc
      661 gatgaattcc gggatgaaac cgaagctaag caaatcctcc ggctcgatgt attgcaaaat
      721 ttgcacgcgg tccaccgaag ctgcgcgatg cccttgttcc acggcattga atcccatcac
      781 atgcttgccg agacggcgct ggatgatgcg ttccaatccg acaaatgctc cgccgcagat
      841 gaacaagatg ttgctcgtat caacgcggat gtattcctgt tgcggatgtt tgcgtccgcc
      901 ctgcggtggc acattgcaaa cggttccttc caaaatcttc agcaaagcct gctgcacgcc
      961 ttcgccggaa acatcgcgtg tgatggaaac gttctcagtc ttgcgcgcga tcttgtctat
     1021 ctcgtcaatg taaacgatgc cgcgttgcgc gcgcttgacg tcgtaatcgg cattttgcaa
     1081 caaacgcaaa atgatgttct cgacatcttc gccaacatag ccggcttcgg tcaatgtcgt
     1141 ggcgtcggca atggcgaacg ggacatccaa aatctgcgcc agagaacgcg ccagcaaggt
     1201 cttgcccgat ccggtcggcc cgagcatgag gatgttgctc ttctcaatct ccacctcggc
     1261 gtggcggtcc aagggcaacg tatcgtcggg ctctccatca ggcggtggcg gttcctggag
     1321 gatgcgcttg taatgattgt gaacggcgac agccaaagtt ttcttcgcgt gctcctggcc
     1381 gacacattgg cgatcaagct gccttttgat ttcagccggc ttgggaatgt tgagcttggg
     1441 cttgtgccgc ttcggttgcg cggagagttc cttgtcgaga acgcttttgc aaaggatgac
     1501 gcagttgtcg cagatgtaaa cgccggggcc ggcaatcaat tttttgactt ccgcgtgcga
     1561 tttgccgcag aagctgcaca gcgtgatatt cgttggccgc gccatggacg attattcctt
     1621 cttgtccgcc aaactcgttg cgtgctcggc cagggtggag atctccttgc gcgattggac
     1681 cacttcatcc accaagccgt aacccttcgc ttcctcggcg gacatgaaat tatcgcggtc
     1741 gcaatccttt tccacctgct cgatggattt cccggtgtgt ttggaaatga tgccattgag
     1801 ccgctgcttg agcttgagca tgtacttgac gcggatttca acatcgctcg ccgggccttc
     1861 cgcgccgcca aggacttggt ggatcatgac gttcgcgttc ggcaatgcgt aacgtttgcc
     1921 cttcgttccg ccgcacaaga gcaccgcgcc catgctggct gcctgcccga tgcaataggt
     1981 gtttacgtcg cacgtgagga attgcatggt gtcataaatg gcgaggccgg cagtgacgct
     2041 gccaccggga gaattgatgt agagatgaat gtcctttttg ggatcattca tttgcaggaa
     2101 caacaattgg gcgatgatga ggttggcgac catgtcatcc acgggcgtgc ccaggaacac
     2161 aattcgatca accaacaggc gcgaatagat gtcatagccg cgttcgccgc gaccggtctg
     2221 ctcgaccacc atcggaacaa gaaaattatc catagtcatc aaaaacactg attattcagt
     2281 ggcgcaacct aacggaggtc gaactccacg tcaagccgcg cgtggcacga accggattgc
     2341 cacttccatg caccctgctg aacacgatca ttcggccaat ctgcaagtgt tttatcttga
     2401 atgaggccat ttttcttgca aggagtgaag gctgaattga actcgctcga cgaacatcgt
     2461 caggtccggc tcatctgcat cgcgctcgcg ttggggactt tgtttcttta ttgccccgtg
     2521 ctgggccatg acttcatcac ctttgatgac tgggcgtatg tgcttgccaa tccccatgtc
     2581 agagacgggt tgagcttgag cggaatctca tgggcgtttg ccggcggcag ctactctgga
     2641 aactggcatc cattgacatg gatttcccac atggtggatt gtcagctcta cgggctggat
     2701 catcccggtg gacatcattt cacgaacgtc ctttttcata tcgcgaacac attgttgctt
     2761 tttgttttgc tgaagcggat gacgggcgcg ttgtggcgaa gcgccgcagt tgccgcatta
     2821 tttgcctggc atccggtcca tgtggaatcg gttgcatgga ttgcggaaag aaaagacgtt
     2881 ttgagcgctt tcttttggct cctgacaatg ctcgcgtatg tgcgctacgc agccgagtcg
     2941 caagcaaagg gacggcggac taaaacttat tacacaatgg cgttgattgg gtttgccctg
     3001 ggcctgatgt cgaagccgat ggtcgtgacg ctcccgttcg tgctgctctt gatggattat
     3061 tggccgctca agcgcggcat ggacccgcag acacccggtg atttgcagtg ggcgcaatgg
     3121 aaaccactgg tgcgggagaa actgccattc atcgcgcttt cgttcatttc gtgcgtggtg
     3181 acattcatct ctcagaagga gggcggagct gtttctactc tcgcgcaagt cccattctgg
     3241 atccgtctct tcaatactca aatctcatac gtcctttatc tcggaaaaat aatctggccg
     3301 acgaagctcg cgattcttta tcccttggag cctctgccgg gctggatgtg gatcgcctcc
     3361 tgtgccgtgc tgctcatcat aagttcactg gcactcatca tgatgcgcag atgcccatgg
     3421 ctttttacag gctggttttg gtttcttgga acgctcgtgc cggtgatcgg cctcgtgcag
     3481 gtgggaaggc aactcatcgc tgaccgctac acttatttgc ccgcggtggg aatttttatt
     3541 gcggtggtct ggggctgcgg agaattcctg gcgcgagtgc cccgattgcg tcccatggca
     3601 attgtcgccg gcgccatggg attgtgcatc tgcatgggca tcacggagaa tcaattgggc
     3661 tattggaagg acagcgtgac gctcttcacc cacgcactcg ctgtggcgcg gcccaatacc
     3721 acggcgctga actgcctcgc gctcggtttg gaaaggcagc acaaaatgga ggaggcgatg
     3781 gccaagtaca agatcgcttt gcaaatcgag ccgcaatcct ttgccacatt tcacttgatg
     3841 ggtctgaacc tcgcctcgcg cggcaattat caggacgccg tcgaatgcta caatcacgcg
     3901 ctcagtttta aagacgaccc caccatccga tacaacctcg gcaacgcgct cgctgcccaa
     3961 ggcaagtatg ccgaagccga ggcgcaatac acacaagcga tacgcgcaga ccctgactcg
     4021 gcggaccttc acaataatct cggcgcgatg ttggcccgcc gcggtttgct ggacgaagcg
     4081 atgcggcatt tccaggcagc cctgcgcttg caacccaact atccggaggc gcaagaccaa
     4141 ttggcggcct tgtttcagaa gcaggggcgg cttgaccttg ccaggatcca ttatgccgag
     4201 gccattcgtt tgaaaccgga ttttgcccac gcgcaactca agctcggcta cgtcatggcc
     4261 caacaaggcg acatcgaggc ggccattcct cattttcaga aggccatcac gctcgaatcc
     4321 accaatgcgg acgcctattt tgatttgggc gccgcttacg ccgccttgga aaattggagg
     4381 ccggctgtgg acgccttctc caacgccgcg caactcaaac cgggcagcgc ggaattcaag
     4441 ggccgccttg ccgaagcccg ggcaaaagct tcgggcgcaa aaaaataaaa tgtcaaagag
     4501 cgggggaagg tgttgttcac cctcccaata attccgcgat tttgccgccg atgtcccgat
     4561 gcttcatcag cgattcgccg accaaaatca cctgcgcgcc gcatttcgcg agacgctcaa
     4621 catcagcgcg ggtatgaatg ccgctttcgg caaccagcaa tttttcgctt ccgttgggcg
     4681 cgccgcgcaa tttggccgcc aaccgttcgg tcgtggcaag gtccaccttg aaacttttca
     4741 ggtcgcggtt gttcacgcct atgacttttg cacccgcggc caacgcccga ttcaattcag
     4801 cttcgtcatg cacttccacc agcgcggtca atcccgcttc tgtggcgagc gcgtgaaaat
     4861 ttttcagttg agcatcggtc aagattgcca cgattaataa aatcgcgtcc gcgccccatt
     4921 caatcgcttc aagaatctgt cgctcatcaa tgatgaaatc cttgcgcagc agcggcaact
     4981 tcactgcctg gcggatttgc ttcaaatatt ccagcgagcc ttggaaaaac tgctcatccg
     5041 tcagcaccga aagaca
//
LOCUS       DASZMM010000286         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5052199, whole genome shotgun sequence.
ACCESSION   DASZMM010000286 DASZMM010000000
VERSION     DASZMM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5052199"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..3612)
                     /locus_tag="VG754_06920"
     CDS             complement(346..3612)
                     /locus_tag="VG754_06920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWF18983.1"
                     /translation="MAKGSLRNGNAARSPGTTKYDAHHTVLSKTYGALADLRRELADS
                     KADVANRTEILTQFSGCGDSQRAWLLLEDYFEKLSLSRKDFTGEEWWPRLMAVEGKTR
                     LEQLAFLFLRAHRPLPTELMPFANLDHFAEVEEAEQEQQLMQQWEQWLFPPTPAHLDS
                     PRASLRVICQTQPDPEQPARNRLGVQFHLFRSRTGEKHKSLSEVIELTTRAAHEQELF
                     QAADWEFIQWLAEAHAGRSDGEATMMLSDLELLQWLARWGHGTRLELAGGLGSLKFEG
                     QVAQLTPHLQNGGTELSFLHQLTLPDGTVQPLSKAQFFSQRPPLALVGNTFYLLRNSP
                     PTTLLEHWSKKPAIPVRKLSHRLLKQLRKARSVPGADWEQLCVAHPALPQFVFELLDE
                     TVRLRLMARSERDQSVWIWNGHEWQPNEPRKSENDKPEILDDPRLEPVTHWLRKLDWF
                     TPEPGLWVGDANENFLSILGAAWAERPDNAEYLGNPAFHRLFLNPRQLRPRVVIKGSG
                     IDWLAVSAEWEQEGMRLTAADLQRLQTASGRFVKLPDSGWVELDSDAVQDAHEAMADL
                     GVDGLIPVPHRIGIEQAAHLGEEELKRFGDTPQAHALRERLKDFKGTPATDLPKTIDA
                     ELRPYQKEGFDFLCHLTQIKLGGILADDMGLGKTLQTLSWLAWLQERHADDPKPALVI
                     CPASVLHNWRREANRFVPHLKVLVLESGAARHNLRKQIPQHDLIVTNYALLRRDLEAL
                     QKFAFRAVILDEAQFIKNPSAQVTQSVKQLRAEHALALTGTPLENRLLDLWSIVDFVQ
                     PGYLGTQEHFTQTYEPRGEDPENAQRIARRRLSAKLRPLMLRRLKQRVAKDLPDRIEE
                     RRDCHLGEDQRKLYLAELRRSREQVMQTVAEKGLNQSKIHVLAALTRLRQICCHPQLV
                     GNDAVSGKTETLFELLDPLLCDGQKVLVFSQFVQVLKLLETECQKRQIRTHLLTGQTK
                     QRQEVVNAFQSDPNAAVFLLSLRAAGTGLNLTNASYVVLYDPWWNPAVEAQAIDRSHR
                     IGQVNTVNAYRLIAPGTVEEKIWELQQSKAQTIADVLGEEGFARSLSKADLEYLFSED
                     "
     gene            3902..>4205
                     /locus_tag="VG754_06925"
     CDS             3902..>4205
                     /locus_tag="VG754_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWF18984.1"
                     /translation="MQFRNLFLALGLLAIPACATTRSAKNIPAGESTAHFNRVITKKA
                     SLDYLVYMPPDAAATPGKPWPAIIFLHGSGERGTNAWDVAKHGPPKIVKDKKDFPFI"
BASE COUNT          898 a         1246 c         1174 g          887 t
ORIGIN      
        1 tgaagcggaa aataatcgtc aggaatgcac aaaagccggg agggattcct tccggctttt
       61 ttggcttaca aatcatctca tcgagaacag agacacatag aacaaattgt ttctcgcaaa
      121 gaccgcgaag atcgcgatgc aggccgggtg ccctcaccgg cgtttcccac catttatcaa
      181 tcgcagatga acgtcgattg acgtagattt gaatgaaaga aaaaagagag aacggagcgc
      241 ggctcgatga gccgcagcaa cacgcgacaa acgggacatg ccagactaat tctgcgcgcg
      301 cctatccttc ccaaattgct aagagtcacc gacccgcgct ccatgctaat cttctgagaa
      361 gagatattcc aaatcggcct tcgaaaggct gcgcgcaaaa ccttcctcac cgagcacatc
      421 cgcgatggtc tgcgctttgc tttgctgcaa ttcccaaatt ttttcctcga ccgtgcccgg
      481 ggcgatgaga cgataggcgt tgacggtgtt gacctgccca atccgatgcg agcggtcgat
      541 ggcctgcgct tcaacggcgg ggttccacca cggatcgtaa aggacaacgt aactggcgtt
      601 ggtgagattc aagccggtgc cggcggcgcg aaggctcagg agaaacaccg ccgcgttcgg
      661 atcgctctga aacgcgttca ctacttcctg ccgctgcttg gtctggccgg tcaaaaggtg
      721 cgtgcgaatc tggcgtttct ggcattccgt ttcgagcagc ttcaacacct gcacaaactg
      781 gctgaacacg agcacttttt ggccgtcgca caaaagcggg tcgagcaatt cgaagagcgt
      841 ctccgtcttg ccggaaacag cgtcgttgcc gaccaattgc ggatgacagc agatttgccg
      901 cagacgtgtg agcgcggcaa gcacatggat cttgctttga ttgaggcctt tttcggcgac
      961 agtctgcatc acctgttcgc ggctgcggcg caattcggcg agataaagtt tgcgctggtc
     1021 ctcgccgaga tggcaatcgc ggcgttcctc gatgcggtcg ggcaaatctt tagcgacgcg
     1081 ctgcttcaag cggcgcaaca tgagcgggcg cagcttggcg gaaagacgcc ggcgcgcgat
     1141 gcgttgcgcg ttttccgggt cttcgccgcg cggttcgtaa gtctgcgtga aatgttcctg
     1201 cgtgccgaga tagccgggct ggacgaaatc cacgatgctc cacaaatcga gcaaacgatt
     1261 ctccaacggc gtgccggtga gcgcgagcgc atgttcggcg cgcaattgtt tgaccgattg
     1321 cgtcacttgc gcggacgggt ttttgatgaa ttgcgcttca tcaagtatca ccgcgcgaaa
     1381 agcaaatttt tgcaacgcct ccagatcgcg ccgcagcaac gcgtaattcg tgacgatcaa
     1441 atcgtgttgc ggaatctgct tgcgaagatt gtgccgcgcc gcgccgcttt ccagcacgag
     1501 cactttcagg tgcggcacga aacggttcgc ctcacgccgc caattgtgga gcacggacgc
     1561 cgggcagatg acgagcgcgg gcttcggatc gtcggcgtgg cgttcctgca accacgcgag
     1621 ccacgaaagc gtttgcagcg ttttgccaag gcccatgtcg tccgcgagaa tgccgccgag
     1681 cttgatttgc gtgagatggc agaggaaatc aaaaccttcc ttctggtatg gccgcaattc
     1741 cgcatcgatt gttttcggca aatcggttgc aggcgttccc ttgaaatctt tcagccgctc
     1801 gcgcaacgcg tgcgcctgtg gagtgtcgcc gaaccgtttc aattcctctt cgcccaggtg
     1861 cgccgcctgc tcgatgccga tgcggtgcgg caccggaatc aacccgtcca cgcccaaatc
     1921 ggccatcgct tcatgcgcgt cctgcaccgc gtccgaatcc agttccaccc agcccgaatc
     1981 gggcagcttc acgaaccggc cgctcgcagt ttgaagacgt tgcaaatccg ccgcagtgag
     2041 ccgcatccct tcctgctccc attccgcgga aaccgcaagc caatcaatgc cgctcccctt
     2101 gatgacgact cgcggacgaa gttgccgcgg gttcaggaac aaacgatgaa acgcgggatt
     2161 gcccagatat tcggcgttgt ccggccgctc ggcccacgca gcgccgagaa tgctgagaaa
     2221 attttcgttc gcatcgccca cccacaaacc cggttcgggc gtgaaccaat caagtttgcg
     2281 caaccaatgc gtcaccggtt cgaggcgcgg atcgtcgaga atttctggtt tgtcgttttc
     2341 gctttttcgc ggctcgttcg gctgccattc gtgaccattc cagatccaga cgctttggtc
     2401 gcgttcgctc cgcgccatca agcgcagccg cacggtctca tcgagcagtt caaaaacgaa
     2461 ttgcggcagc gcgggatgcg cgacgcagag ctgctcccaa tccgcgcccg gaacagaacg
     2521 cgccttgcgc aattgcttga gcaaacggtg gctcaacttg cgcaccggaa tcgccggttt
     2581 ttttgaccaa tgctccagca gcgtcgtcgg cggcgaatta cgcagcagat aaaatgtgtt
     2641 gccgaccagc gcaagcggcg gacgttgcga gaaaaattgc gccttgctca acggttgcac
     2701 cgtgccatcc ggcagggtca attgatgcaa aaaggaaagt tccgtgccgc cgttttgcag
     2761 atgcggcgtc aactgcgcca cctgcccttc aaatttgagc gagccaagcc cgccggcaag
     2821 ctcaagccgc gttccgtggc cccaacgcgc aagccattgc agcaattcga ggtccgacaa
     2881 catcatcgtc gcttcgccgt ccgaacggcc cgcgtgcgct tcggccagcc attggatgaa
     2941 ttcccaatcg gcggcttgaa aaagttcctg ttcgtgcgcg gcgcgggtgg tcagctcgat
     3001 gacttcagac agcgatttgt gtttctcgcc tgtgcgcgag cggaaaaggt ggaattgcac
     3061 gccgaggcga ttacgcgccg gttgctccgg gtcgggctgc gtctggcaaa tcacgcgcaa
     3121 cgaggcgcgc ggcgaatcaa gatgcgcggg cgtgggcgga aaaagccatt gctcccattg
     3181 ctgcatcaac tgctgctcct gctccgcctc ctcgacctcc gcgaaatggt cgagattggc
     3241 gaacggcatc aattccgtgg gcaacggacg gtgcgcgcgc aggaacagga aagccagttg
     3301 ttcaaggcgc gttttgcctt cgaccgccat caggcgcggc caccactcct cgccggtgaa
     3361 atccttgcgc gacagggaaa gtttttcgaa ataatcctcc agcagaagcc atgcgcgctg
     3421 ggaatcgccg cagccggaga attgcgtgag tatttccgtg cgattggcca catccgcctt
     3481 ggaatcagcc aattcccgac gcaagtcagc gagcgcaccg taggtttttg acagaaccgt
     3541 atgatgagcg tcgtacttcg ttgttccggg cgaacgcgcc gcgttcccat ttcttaacga
     3601 accttttgcc attctgattg attaacctgc tgcaagcagc aagcgtttat catctcctca
     3661 caaatcaccc atgctggtca atcatatttt gaaatatttg ggttgttggg gaaaagaccc
     3721 cattggaatt tacgaatcaa gatttgcgat tttatccgga tgcggaggat ttcgcttcaa
     3781 ctacaagtca tctctaaagc gagaacggag cgcggctgtg tccgcaggac cagccgcagc
     3841 atgacggctg gactttccga ttgacactcc caaactcccg cgcaaatgtt gcgttcaact
     3901 gatgcaattc cgtaatttat ttctcgcttt gggccttttg gcgattcccg cctgcgcaac
     3961 cacccgcagc gcaaaaaata ttcccgcggg cgaaagcacc gcgcatttta accgcgtcat
     4021 cacgaaaaaa gcgtcgctcg attatctggt ctatatgcct ccggacgccg ctgcgacgcc
     4081 gggcaaacca tggcccgcaa tcattttcct gcacggctcc ggcgagcgtg gaacgaatgc
     4141 ctgggacgtc gccaagcacg gcccgcccaa aatcgtgaag gacaaaaagg attttccctt
     4201 catcg
//
LOCUS       DASZMM010000287         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4784506, whole genome shotgun sequence.
ACCESSION   DASZMM010000287 DASZMM010000000
VERSION     DASZMM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4784506"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..918
                     /locus_tag="VG754_06930"
     CDS             238..918
                     /locus_tag="VG754_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF18985.1"
                     /translation="MNNKAATDRGEVRILLVDDHPLVRLSLREVIKREPDLLVCGEAE
                     DREQALELVESAKPHLAIVDLTLKSSNGMDLIKDLRSRHPKVQILVLSMHDETLHAER
                     AIRAGARGYITKQEATKKIMVAIRQILSGEIYWSERAAQRVASKIAGGSKASTGSSVD
                     LLADRELQVFELIGAGQSTRQIAAALHIDVSTVETYRARIKEKLNLEDSLALLQFAIR
                     WHIANNQA"
     gene            complement(1072..2502)
                     /locus_tag="VG754_06935"
     CDS             complement(1072..2502)
                     /locus_tag="VG754_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF18986.1"
                     /translation="MKPLQSWNRRDFLKTASAATLGALAAGYPKQILAAEAKEEKIKP
                     TADTVIVLWMAGGMAHTETFDPKRYTAYEKGLGSNKVLSTFPAIDTKVDNIKISQGLE
                     KIAGVMDRATLIRSYTAGDLGFILHSRHQYQWHTGYAPPQTVAAPHIGSVIARTLGPL
                     NPAVPAFIDIGQRLDLGEGEELKAFHTAGFLGSEYGPFFIANPEQAVESVRPPAGMSP
                     SRFENRDKFYRKLAEKSPISQYGSDYQQQSLLRSLDNAHRLLSSPASKAFDLSLEPKE
                     SYNNYNVSQFGLGCLLARRLTEVGARFIEVTTEYIPFLNWDTHENGHTRAVDMKKSID
                     APIAQLVLDLEARGLLDRTLIVLASEFSRDMMMEGKPGNLVKNQVVVPDVINEMKFYG
                     MHRHFTDAGCVLMFGGGVKKGHLHGKTADERPCTTVAQRVVIEDLHASIYRAVGISPK
                     LSYEIEKRPFYVTKDGLGKPVEELFA"
     gene            3070..3528
                     /locus_tag="VG754_06940"
     CDS             3070..3528
                     /locus_tag="VG754_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF18987.1"
                     /translation="MRRGTCETWKVTARAEFSMDGCKRFPASRWPGFADAGWHPLCAP
                     CLTRAEYFTAEKNVQLFMGSVGFLRSLGLVLASDSGDSFAMEIVFSVTQENDGGYVAE
                     CLSYDIFTQGDTWDELRANVREAVSAYFFDQAKPDAIRLHLVRDELLAGK"
     gene            complement(3963..>4237)
                     /locus_tag="VG754_06945"
     CDS             complement(3963..>4237)
                     /locus_tag="VG754_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HWF18988.1"
                     /translation="CGFDYQLVSDKPENFLAIEVKGLRERSGSIMLTEKEHKVAEVLK
                     ERFFLFIVFNFREKPFHRIFNKPLVGPLKFSKKERLVKEITWSAAC"
BASE COUNT          983 a         1110 c         1120 g         1024 t
ORIGIN      
        1 cggccataaa gaaaattgcc gatggcgaac aatccggcgc caaggcaaga caaatgaaaa
       61 ttgaatacac aatcatgtgc ctgttgtcac attgttgttt cttgccatga tgcgccgaat
      121 ctgcgttaat gatttccgag gtgagaggta atcaacacct cagatacttg aaagcccggc
      181 gcaaatgaat aaaattttat tatggcaaag tgtcgggaca aattaattgc gggcgacttg
      241 aataataaag cggcaacgga tcgcggagaa gtccgcatcc tgctcgttga tgaccatccc
      301 ttggtccgtt tgtccctgcg cgaagtcatc aaacgcgaac ccgatctgct cgtttgcggc
      361 gaagccgagg accgcgagca ggcattggaa ctggttgaaa gcgccaagcc gcatctcgcc
      421 atcgtggacc tcactttgaa aagctccaat gggatggacc tcatcaagga ccttcgcagc
      481 cgccatccca aggtgcagat actcgtcctc tcgatgcacg atgaaacctt gcacgccgaa
      541 cgcgccatcc gcgccggggc gcgcggttac atcaccaagc aggaagcgac caaaaaaatc
      601 atggtcgcca tccgccaaat tttgtccggc gaaatttatt ggagcgaacg cgccgcgcaa
      661 cgcgtggctt cgaaaatcgc cggcggctcc aaggcgtcca ccggttcttc ggttgatctt
      721 ctcgccgacc gcgagttgca ggtcttcgaa ctcatcggcg cgggccaaag cacccgccag
      781 atcgccgccg cgctgcacat tgatgtcagc accgtcgaga cctaccgcgc gcgcatcaag
      841 gagaaactca acctcgaaga ttctcttgcg ctgctccagt tcgccatccg ctggcacatc
      901 gccaacaatc aagcgtagat tgattgaatt ttgacttgtt tgattccccg ggcactggag
      961 aaaacatcga acagccaaca ctgaacgctg aacatcgaat ctcggaagct caccggcatt
     1021 cgattggatg ttgtgcattc gatgttcgtt gttcgatgtt ccctgacaat cctacgcaaa
     1081 caactcctcc accggcttgc ccaagccgtc cttggtgaca tagaacggac gtttctcaat
     1141 ctcgtaactc agcttgggcg aaatgccaac tgcgcggtaa atcgacgcgt gcaaatcttc
     1201 aatgacgacg cgttgagcga cagtcgtgca aggacgttca tccgcagtct tgccgtggag
     1261 atggcctttc ttcacccccc cgccaaacat cagcacgcaa ccggcatccg tgaaatgccg
     1321 gtgcattccg taaaatttca tctcgttgat gacatccggc acgacaacct ggtttttgac
     1381 gagattgccc ggtttgcctt ccatcatcat gtcgcggctg aattcgctcg cgagcacaat
     1441 caacgtgcgg tcgagcaaac cgcgcgcttc cagatcgagc accaattgcg cgatcggcgc
     1501 gtcaatgctc ttcttcatat ccaccgcgcg cgtgtggcca ttctcgtgtg tgtcccaatt
     1561 caaaaaagga atgtattcgg tggtgacctc gatgaaccgc gcgccgacct ccgtaagacg
     1621 ccgcgcgagc aaacaaccaa gcccgaactg gctcacatta tagttgttgt aactttcctt
     1681 cggttcgagc gaaaggtcga acgccttgga cgccggcgaa ctgagcaaac gatgagcgtt
     1741 gtcgagcgaa cgcaacagcg attgctgctg ataatcgctg ccgtactggc tgatgggact
     1801 tttttcggca agtttgcgat aaaatttgtc gcgattttcg aagcgggacg ggctcatccc
     1861 ggcaggcggg cggacgcttt caacggcctg ctcgggattt gcgatgaaga acggaccgta
     1921 ttcgctgccg agaaaaccgg cggtgtgaaa cgccttgagt tcctcgcctt cgccaaggtc
     1981 caaccgctgg ccgatgtcaa tgaacgcggg caccgccggg ttcaacggcc cgagcgtgcg
     2041 cgcgatgact gaaccgatgt gcggcgcggc aaccgtctgc ggcggcgcat aaccggtgtg
     2101 ccattgatat tgatgccgtg aatgcaggat gaaaccgagg tcgcccgccg tgtaagaacg
     2161 gatgagcgtg gcgcggtcca tcacgcccgc gattttttcc aagccctgcg aaatcttgat
     2221 gttatcgacc ttcgtatcaa tcgccgggaa agtggaaagg actttgtttg atcccaatcc
     2281 tttttcgtaa gcggtgtaac gcttgggatc gaacgtctcc gtgtgcgcca tgccgccagc
     2341 catccaaagg acgatgacgg tgtcggcggt cggtttgatt ttttcctcct tcgcctcggc
     2401 agcgaggatt tgtttcggat aacccgcggc gagcgcgccc agcgtggcgg cgctggcggt
     2461 tttcaggaag tcgcggcggt tccaagactg aagcggtttc atagttgaac aatacacaaa
     2521 ttcaaagtgt aatgccagtc cccaaaaagg aggcgtaggt caagccttga cccgatttgg
     2581 ctgaaaatgg agtgttggaa tggcggagta ttggtgaaca ggcggttgaa tgctttagca
     2641 tgtttctttc aagttgcctc catacagaca cgctgaagcg tggacaccga accgttgccg
     2701 cgcggaagcg ctccacaatc cgcgttcgca atggggggag ggggtggtat tttggccggc
     2761 tttaagtgat tctaagcggg ttttcaagta cttacgcatg gtgatgaagt gactgggtac
     2821 atccgcgaaa gcagcgagca tggaggatgg tgacggatgg agcgtcgtct tcgatccggc
     2881 aaattgtttg gtgtccatgc tttagcatgt ttctttcaag ttgactccag acagacacgc
     2941 tgaagcgtgg acaccgaacc attgccacgc ggaagcgctc cacaattcgc gttccgcaat
     3001 ggggggtgcc aatttcggcg gattaaagcg ggtctaagtg attctaagcg ggttttcaag
     3061 tacttacgca tgagacgcgg aacttgtgag acttggaaag taacagcgcg cgccgaattc
     3121 agcatggatg gatgtaaacg atttcccgcc agccgatggc cgggtttcgc agatgccgga
     3181 tggcatcctc tgtgtgctcc ttgcctcaca cgcgctgagt atttcacagc agaaaaaaat
     3241 gtccagctat ttatgggttc tgtcggtttt ctcaggagtt tagggcttgt tttagcctca
     3301 gattccggcg atagttttgc catggaaatt gtcttcagcg tgacacagga aaacgacggc
     3361 ggctatgtgg ccgaatgtct ttcttatgac attttcaccc aaggcgacac ttgggatgaa
     3421 ttgcgagcaa acgtgcgcga ggcagtgtcc gcttatttct tcgatcaggc caaacccgat
     3481 gcaatacgcc ttcatctggt gcgcgacgag cttttggccg gcaaatgaaa ctgccgcgtg
     3541 accttagcgg cgcagagctg atcaagttgc tttgcaagca ccatggctat agacgcgtga
     3601 atcaggaagg aaggagtttt gttaacagat cgcgagtgca attcatgtgg acaagcagcc
     3661 gcttatctgg ttcgaatcat gcccagcgat tttgacctga tcagctcttc gactcggttc
     3721 ttcgttgacc cttcgtcaaa tgctgtttct ctctaactcg agtcctttct gataaagttg
     3781 gcgaaattgg acttgttggt tcttcatcgc agagtcgcac gcaatctcca caattgccat
     3841 ggcgttgctg atgtcatcat ttgttccacg caggtccaat tcgcctattg cggataaaat
     3901 gacgtttttt aatctgcgta ttcttttgac agacgcgtgt tcgtattttc ttttcatacc
     3961 tatcaacaag cagcggacca tgtgatttct tttacaagtc tttccttctt ggaaaattta
     4021 aggggcccga caagaggttt gttgaaaatc cggtgaaaag gcttttctcg gaaattgaaa
     4081 acgatgaaaa gaaaaaacct ttcctttaat acttcggcta ctttatgctc cttttctgta
     4141 agcataatgc ttcctgatcg ctcccgtaag cctttaactt caatggccag aaaattttcc
     4201 ggtttatccg aaaccaattg ataatcgaat ccgcatc
//
LOCUS       DASZMM010000288         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2587058, whole genome shotgun sequence.
ACCESSION   DASZMM010000288 DASZMM010000000
VERSION     DASZMM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2587058"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1617)
                     /locus_tag="VG754_06950"
     CDS             complement(67..1617)
                     /locus_tag="VG754_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF18989.1"
                     /translation="MTNNDFELLQEYAQKDSEDSFAELVRRHLNMVYSAALRQVRSQQ
                     LAEEVAQSVFADLARNARRLAPGAIITAWLYQVTRRTAIDVIRRESRRQLREQIAAQM
                     NAIHNTEAEWLQIEPLLDEAMESLDETDRAAILLRYFENKPLREVGVTLGISDDAAQK
                     RVSRAVERLQEFFKQRGLGIGAAVLVVLISANAVQSAPIGLVLAISAAAATGTSLATT
                     AMTAKTIATMTAMQKSIVLASLIVAAGMGIYTTGKTSQLRNQVKSLTGQQDALSTRLA
                     QAEQERDASTNRLASLNQQLNELKSNQNSAELLRLRGEVSVLRKERQEQSRDKMALNT
                     EDPATQSFLAARALAQKISDAFQQMPEKAIPELKLMTDVDWLSATKQASFDSDADTRK
                     TLRNVRALAKKRLPLGGSLFSFTQANNGQLPSSLSQLKSYFLSQLNYSSRETAQLDDG
                     TLDAILSRYELLHSGNVSDYPPGTWFIGEKSSVDKDYDSRAKFGNGMDTVIDTGLNEN
                     GDPDDPSY"
BASE COUNT          411 a          475 c          566 g          450 t
ORIGIN      
        1 ggccgtggaa aaaatccatt tcgatggggc gcattttcgg cgggacatcg ccgacaaagc
       61 aatccatcag taagacggat cgtcgggatc gccattttca ttcagaccgg tgtcaatcac
      121 cgtgtccatg ccattgccaa acttcgcgcg tgaatcgtag tctttatcca ctgatgattt
      181 ttctccgatg aaccaggtgc ccggaggata gtcgctgaca ttcccgctgt gcaacaattc
      241 gtaacgagaa agaatcgcgt ccaaggtgcc gtcatccagt tgggcggtct cccgggagct
      301 atagttcaat tgagacaaga aatatgattt caattgagag aggctggagg gtaattgtcc
      361 gttgttggct tgagtgaaag agaacagcga accacctaga ggcaaacgtt ttttggcgag
      421 agcgcgaaca ttgcgcaaag ttttgcgggt gtcggcatcg gaatcgaagc tggcctgctt
      481 ggttgcgctc aaccaatcaa catcggtcat aagctttagt tccggaatgg ctttttcagg
      541 catctgctga aatgcatcgg aaattttctg cgcaagtgcc ctggcggcca ggaacgactg
      601 tgtggccggg tcttctgtgt tcaaggccat tttgtcccgc gattgttcct ggcgttcctt
      661 cctcaacacg gacacctcgc cacgcaagcg aagcagttcg gccgaatttt ggttcgattt
      721 caattcgttg agttgctggt tgagtgaagc gagccggttc gtggacgcgt cgcgttcctg
      781 ctccgcctgg gcaagtcggg ttgaaagggc gtcttgctgg ccggtcaaac ttttgacctg
      841 gtttcggagt tgggaggttt tgcctgtcgt gtagatgccc attcctgcgg caacaatgag
      901 agaggcgagc acgatggatt tttgcatggc agtcatggtg gcgatggttt tggcggtcat
      961 tgccgttgtg gcaaggctgg tcccggtcgc ggcggcggcc gagatggcaa ggaccagccc
     1021 gatgggggcg gattggacgg cgtttgctga gatgagaaca acaagcacgg cagcgccaat
     1081 cccgaggccg cgttgtttga aaaattcttg aaggcgttcg acggcgcggc tgactcgctt
     1141 ttgcgcggca tcatcggaaa tcccaagcgt gacgccgact tcgcgcaacg gcttgttttc
     1201 gaaatatcgc agcaggatgg cggcgcgatc ggtttcatcc agcgattcca tggcttcgtc
     1261 gagcagtggc tcgatctgca gccattcggc ctcggtgtta tgtatggcat tcatttgagc
     1321 ggcgatttgt tcgcgcaatt ggcggcggct ctcgcggcgg atgacatcaa tggccgtgcg
     1381 gcgagtgacc tgataaagcc atgcggtgat gatggcgcca ggagccaagc gccgggcatt
     1441 ccgggcaaga tcggcaaaaa cggattgggc gacttcctcc gccaactgtt gcgagcgcac
     1501 ctgccgcagc gccgctgaat aaaccatgtt cagatggcgg cgaaccagtt cggcaaatga
     1561 gtcttccgaa tctttttgag catattcctg aagcagctca aaatcgttat ttgtcatttc
     1621 aaggagtata tcggcaccgc caggcgaaat ccgacaaaat accggcctaa aatccgcagg
     1681 ttcgccaaaa agccgccgga cgcgtatcca agacttgacc gcgccgctta gttgggcata
     1741 caaaggcgtc gttcgcaggc ggtttagtcc tgcaagattc aattcgacgg cgcgcaattt
     1801 agacgcgata ttgccgccaa ggcgctgcga taacaggggt ttcccaacgg cagcccggca
     1861 gagcaaccat tatgaaattt tttagaaaga tgttttttgg gg
//
LOCUS       DASZMM010000289         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4249593, whole genome shotgun sequence.
ACCESSION   DASZMM010000289 DASZMM010000000
VERSION     DASZMM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4249593"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VG754_06955"
     CDS             complement(<1..1187)
                     /locus_tag="VG754_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF18990.1"
                     /translation="MNTYSYSAIDAEGRETRGTIQVADQSEALKRIKEMGFFPVKVTE
                     IPHEPLTPAARVLRHGKRLGLTSRAGKPSQPRYAKRARKKTITLFTRQLATLLEAGMP
                     VLRSLRLLHEQETDRPFKPILAGLASAIEGGSSFSEALAQHPKIFDRLYLNMVKAGEL
                     AGMLETSLKRLADFMEKAARIKGKVKAAMFYPAAVMTVATGVMALMLLFIVPRFKEVF
                     EGLGNGRPLPAFTRFVLGISEAVKSHFLFTAALCAALYGIFLIVVQTRPGRRFFDDFK
                     LRAPVVGQVFRKLSIARFTRTLGTLITSGVPILQALAIIKDATGNVVVGQLVARVRKN
                     VEDGESIVGPLRESHIFPAMVVGMVDVGEQTGALPEMLNKIADTYDEEVDNSVTAMTS
                     LLEP"
     gene            complement(1293..>1643)
                     /locus_tag="VG754_06960"
     CDS             complement(1293..>1643)
                     /locus_tag="VG754_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF18991.1"
                     /translation="LAQQGHADAAIAEFQHATELDPANSIARADLASLQIPNGKLDDA
                     IANLRAAAKYDPENADLHNKLGALLAQTGQYEPAITEFQAALKLNPSLPRAAENLALA
                     QKLKDKPSGKPWLK"
BASE COUNT          332 a          442 c          498 g          371 t
ORIGIN      
        1 ggctccagca atgacgtcat ggcggtcacg gaattgtcca cttcctcgtc gtaagtgtcg
       61 gcgattttgt tgagcatttc cggaagcgcg ccggtttgtt cgccgacgtc caccatcccc
      121 accaccatcg cgggaaaaat atgcgattcg cgcaacggcc ccacgatgct ttcgccatcc
      181 tcaacatttt tgcgcacgcg cgcgacgagt tgcccgacga ccacgtttcc ggtggcgtcc
      241 ttgatgatgg cgagcgcctg caagattggc acgccgctgg tgatcaatgt gcccagtgtg
      301 cgggtaaacc gcgcgatcga caatttccgg aacacctgcc cgaccaccgg agcgcgtaat
      361 ttgaaatcat cgaaaaaccg ccgtccggga cgcgtttgga ccacgatcaa aaaaatcccg
      421 tacaaagccg cacaaagggc tgcggtgaaa agaaaatggc ttttcaccgc ctcgctgatg
      481 cccagcacga accgggtgaa agcgggcagc ggacggccat tgccgagtcc ttcaaagact
      541 tccttgaacc gcggcacgat gaacaacagc atcaacgcca tcacgccggt cgcaaccgtc
      601 atgaccgcgg ccggataaaa catcgccgcc ttcaccttgc ccttgatgcg cgcggctttt
      661 tccatgaaat cagcgagccg cttgagcgaa gtttcgagca tcccggccag ttctccggct
      721 ttgaccatgt tcaggtaaag ccggtcgaaa atttttggat gttgcgcgag cgcttcgctg
      781 aaactgctgc cgccttcgat ggccgaggcc aacccggcaa ggatcggttt gaaagggcgg
      841 tcggtttcct gctcgtgcaa aagccgcaaa cttcgcaaga ccggcattcc tgcttccagc
      901 aacgtcgcaa gttgtcgcgt gaacaaagtg atcgtctttt tgcgcgcgcg ctttgcgtaa
      961 cggggttgtg aaggtttgcc ggcgcgagaa gtgagaccga ggcgcttgcc gtggcgcagg
     1021 acgcgcgcgg cgggcgtaag cggctcgtgg ggaatttcgg tcactttgac gggaaaaaat
     1081 cccatctcct tgatgcgctt gagcgcttct gattgatcgg cgacttgaat tgtgccgcga
     1141 gtctcgcgcc cctcggcgtc gatggcggaa tacgaataag tgttcatgcg gtttcctccc
     1201 ttggttataa tcatatggac ttatgagctg tgcagttatg gtaatgagtc tggcaggaat
     1261 cgcaagtgaa aaatgcgcag aacccgggcg ttttatttca gccaaggttt gcccgaaggc
     1321 ttgtctttca gcttttgagc gagggcaagg ttttcggcgg cgcggggaag cgatggattc
     1381 aatttcagag cggcttggaa ttccgtgatg gccggttcgt attggccggt ttgagcgaga
     1441 agcgcgccga gcttgttgtg aaggtcggca ttttccgggt cgtattttgc tgcggcacgt
     1501 aaatttgcga tggcgtcatc aagttttccg tttgggattt gcagcgaggc aaggtccgcg
     1561 cgggcgatgg agttggcggg gtcgagttca gttgcgtgtt ggaattcagc aatcgcggcg
     1621 tcggcatgac cctgctgggc gag
//
LOCUS       DASZMM010000290         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3672318, whole genome shotgun sequence.
ACCESSION   DASZMM010000290 DASZMM010000000
VERSION     DASZMM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3672318"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VG754_06965"
     CDS             complement(<1..1254)
                     /locus_tag="VG754_06965"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWF18992.1"
                     /translation="MLCSRAETSAWASVSTSAPVQYHVRTWQAEEGLPQDAVQALAQT
                     RDGYLWVGTKKGLARFDGVHFKVFDPQNTPELRNASITALLQSADGSLWIGTAGGGLV
                     QMKDDKFFFHDLSGEIRGNTAKTFLQARDGTLWVGTLDGLFKMQDGHWTHLTRPDTRN
                     VIISLEQSDDQLWIGTATGVSVLRNGSFSEMKEIGDRARTIYKDRDGTIWFGLTAGLG
                     CLPRNGVFKFYTRKDGLPDNIVSAIFQDKRGDLWIGTYSGLCRFRDGKFITELDSLGT
                     FYDQVNVITEDQEGDIWVGTRDGLHELKVKRFLTYTRQNGLAHNNILSVLEDHKGNIW
                     LGTWGGGVSRLNEEGIVNFNWQNSLANGLTSDLILSLCEDKDGSLLIGTDYEGGTFRM
                     EGNSFKRVWDQKQAAVDRIIRVIYRD"
     gene            1549..3156
                     /locus_tag="VG754_06970"
     CDS             1549..3156
                     /locus_tag="VG754_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWF18993.1"
                     /translation="MVSKTVLAAGTWTPLVNLPPAFLDTMLLLSDGTVMATDGGNGWY
                     RLTPDIHGSYVYGTWSLLAPMNDTRLYFASDVLRDGRLFVAGGEYGTGSGTAEIYDPL
                     TNGWTELPTSSQNFDDAISTVLPDGDVLIAPLNPSVPGTAVIYNVVSNTWSIAPSYFQ
                     GNGTVVNQQEASWVKLPDDSILTIDPSTINSERFIPSLNEWIGDAAVPVFLFDGRSEL
                     GPAVLLTNGQVFFTGASGHTALYTPSGNTTPGSWTAGPDIPDGLVAADAPCAMLVNGK
                     VLCAFGPSDWSIQSTSFLEYDPASNAFNAIAGPTGTNYPMVAYGMRMLDLPDGTVLLS
                     VSGPNLYVYRPDGSPIAAGKPAITSITTNADGSYFLSGKLLNGISSGAAYGDDAQMDS
                     NYPLVRMTDANGNVYYARTANWSSTGVMTGNAVVTTQFWLPASLPPGTYSLVAVANGI
                     GSDPVLFPYNTATIIVRAAIINGGFALSWNAVVGQSYHIQYETNSTQTDWLDLVGAIT
                     ATNYIISVTDTNVNAASPQKFYRIVSP"
     gene            3256..>3838
                     /locus_tag="VG754_06975"
     CDS             3256..>3838
                     /locus_tag="VG754_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HWF18994.1"
                     /translation="MRAWSSGLVSLWLMLFAVVFSTAADQAFSFATTPGQLPKNVVPH
                     HYAMRIEPDLQKFTTRGSMSVRIEVVKPVKQILLNAVDLKVTKATLESSSLRKSDLKV
                     KVVPEKQLLVLKSSKEIAPGTYQLALEFSGKIIEQAEGFFYAKYSATNGDKVMLATQM
                     EPTDARRMFPCWDEPVFRATFDLTVVLPENFKAF"
BASE COUNT          867 a         1084 c          973 g          914 t
ORIGIN      
        1 gtcgcggtaa atcacccgga tgatgcggtc aacggctgct tgtttttggt cccaaacccg
       61 cttgaaagag ttcccttcca tgcgaaatgt cccgccctcg taatccgtgc cgatcagcag
      121 gctgccgtct ttgtcttcac aaagagaaag gatgaggtcc gaggtcaaac cgttggccag
      181 gctgttctgc caattaaaat tgacgattcc ctcctcattc aaccggctca cacctccgcc
      241 ccacgtgccg agccagatgt tgcctttgtg gtcttcaaga actgacagga tattgttatg
      301 ggcaagacca ttctgccggg tataagtcaa gaatcgtttg actttcagtt cgtgcaaacc
      361 atcgcgtgtg ccgacccata tatcgccttc ctggtcctca gtaatcacgt tcacctggtc
      421 gtagaatgtg cccaggctgt ccaattccgt tatgaacttt ccatcgcgaa accggcacaa
      481 tccactatac gtgccgatcc acaagtcccc gcgtttatct tggaaaattg cgcttacgat
      541 gttgtcgggc aatccatctt tgcgtgtgta aaacttgaag acaccgtttc ggggaaggca
      601 tcccaacccg gcagtcaacc caaaccaaat tgtcccatcc cgatctttgt aaatcgttcg
      661 cgccctgtcg ccgatttctt tcatctccga gaaagagccg tttcgcaaga cggacactcc
      721 tgtggccgtg ccaatccaga gctgatcatc cgattgctcc agcgagatga tcacattgcg
      781 cgtatccgga cgagtcagat gtgtccaatg tccatcctgc atcttgaaaa gcccgtcgag
      841 cgtgcccacc cataaggtgc cgtcgcgggc ctgcaaaaat gtcttcgctg tgtttccccg
      901 aatctcaccg ctcagatcgt ggaaaaaaaa cttgtcgtcc ttcatttgaa caagcccccc
      961 gcctgcggtg ccgatccata agcttccgtc ggcactttgg agcagcgcgg ttatggaggc
     1021 attcctcaat tccggtgtgt tctgcggatc aaaaactttg aagtgaacgc catcgaatcg
     1081 cgccaaaccc ttctttgtgc cgacccacaa atatccgtcg cgcgtttgcg ccagcgcctg
     1141 cacggcatct tgcggcaatc cttcctctgc ctgccatgtc cgaacatgat attggacagg
     1201 cgcgcttgtg gaaacggagg cccaagctga agtttccgcg cgcgagcaca acatccatat
     1261 aaacatggga gcaagccaac gagcgcggac aaaaatatcg tggcgagcga acttcatcgt
     1321 gcgcttagtt atacgtcttt tgacatcaaa tgaaagccgg aattggcgcg cgcggttggt
     1381 ccaaattttt gctcgaaatc ctgctaacct gccctatcct gtcaggtagc ttcagagaaa
     1441 cgggatttag ctaaacagaa ccaaccggga agcatgttgt ttactgacgc acagactcac
     1501 cgctgcattc gcggcagggg catgcttttg tccgttattc tgggcttgtt ggtttcaaaa
     1561 accgtcctgg ctgcaggcac atggacaccg ctcgtcaacc ttcctcccgc ttttttggat
     1621 acaatgctgc tgctctccga cggaactgtg atggccacgg acggcggtaa cggttggtac
     1681 agacttacgc ccgatattca cgggagctac gtgtacggca catggtcttt attggcgccc
     1741 atgaacgaca cgcgccttta ttttgcttcc gatgttttgc gcgatggcag gttgttcgtt
     1801 gccggtggag aatacggcac cggctcgggt accgccgaga tttatgatcc gctcacgaat
     1861 ggctggacag agttgccgac ttccagccaa aacttcgatg atgccatttc aacagttttg
     1921 cccgacggcg acgtgctcat cgctccctta aatccttccg tcccaggcac ggcggtcatt
     1981 tacaacgtcg tctcgaacac ctggagcatc gcgccgtcct attttcaggg caacggtacg
     2041 gtagtcaatc aacaggaagc cagttgggtt aagttgccgg atgacagcat tctcacgatt
     2101 gatccttcga caatcaactc ggagcggttc attccgtctt tgaacgaatg gattggcgat
     2161 gcggctgttc cggtattctt gttcgacggc cgcagtgaac tgggacccgc ggtgctgctc
     2221 accaatggcc aggttttttt cacgggtgcc agcgggcata ctgcgctcta cacgcccagc
     2281 ggcaacacaa ctccaggttc atggacagcc ggaccggaca tccccgatgg cctcgttgca
     2341 gcggacgcgc cttgcgcaat gctggtcaac ggaaaagttc tttgcgcctt cggcccatct
     2401 gactggtcca tccagagcac cagcttcctc gaatacgatc cagcgtcgaa cgcctttaat
     2461 gcgattgccg gcccgacggg aaccaactac ccgatggtgg catacggaat gcggatgctc
     2521 gatttgccgg acggaacggt gctgttgtcc gtttcaggtc caaaccttta cgtgtatcgt
     2581 ccggacggtt ctccgatcgc tgccggaaaa ccggccatca caagcataac aaccaacgct
     2641 gatggctcat actttctctc cggcaaattg ctcaatggaa tttccagcgg cgcggcgtac
     2701 ggagacgacg cccaaatgga cagcaactac ccgctcgtgc gcatgaccga tgcgaacgga
     2761 aatgtttatt acgcgcgcac ggcaaattgg agcagcacag gcgtcatgac cggaaatgcg
     2821 gtggtgacga cacaattctg gctgcccgca tcattgcccc cgggaactta ttcattggtg
     2881 gcagtggcga acggcatcgg ttcggatccg gttttatttc cttacaacac cgctacgatc
     2941 atagtaagag cagcgattat aaatggcgga ttcgccctct cttggaatgc cgtcgtggga
     3001 caaagctatc atatccaata cgaaaccaat tcgacacaga ccgactggct cgatttggtt
     3061 ggcgccatca ccgcgacgaa ctacatcatc tcagtgactg atacgaacgt caacgccgcc
     3121 tctccgcaga aattctatcg catcgtgagt ccgtgaaccg gcatgaaaat ctcgatgcga
     3181 gaacgtccgg catgatttca tgctgcatga gccaacttca ttgacgcgcg aggggctttg
     3241 attgattgtc tcgccatgcg tgcatggagc agcggtttag tttctctttg gttgatgttg
     3301 tttgccgtgg ttttttcgac ggcggcagac caggcgtttt cgttcgcaac cacaccggga
     3361 caattgccca aaaacgtcgt tccacaccac tatgcaatgc gcatcgagcc tgacctgcaa
     3421 aaattcacca cgcgtggctc gatgtccgtg cgcatcgagg tggtgaaacc agtgaagcaa
     3481 atcctgctta acgctgtgga tctgaaagtg accaaggcaa cattggaaag cagttcgctt
     3541 cgcaaatccg atctcaaggt aaaagttgtg cccgagaaac aattgcttgt gttgaaatct
     3601 tcaaaggaaa tcgcgccggg cacttatcag ctcgcattgg aatttagcgg caaaatcatt
     3661 gagcaggccg aaggattttt ttacgccaaa tattcggcga cgaatggcga caaggtgatg
     3721 cttgcgacgc aaatggaacc aaccgatgcg cggcgcatgt ttccctgctg ggacgaaccg
     3781 gtctttcgcg ccacgttcga cctgaccgtg gttcttccgg aaaatttcaa ggcctttt
//
LOCUS       DASZMM010000291         6035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3135086, whole genome shotgun sequence.
ACCESSION   DASZMM010000291 DASZMM010000000
VERSION     DASZMM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135086"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..1638)
                     /locus_tag="VG754_06980"
     CDS             complement(454..1638)
                     /locus_tag="VG754_06980"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWF18995.1"
                     /translation="MRNQSRSGRLSHHVIGLMSGTSADGIDAVVAEISGTGRGLKARL
                     IAHHQQAFAPLLRKHILHICLHGTVAEICELNFLMGEHFARAALAVIRQAGLKPEQIA
                     AIGSHGQTIHHLPRAKTPSTLQIGEPCVIAERTGITTVADFRVRDMAAGGQGAPLVTY
                     VDWALQTDSARPRILQNIGGIGNLTFLPPRAALQEVMAFDTGPGNMVMDAVVNELSSG
                     KVRYDRNGQWAAKGKVSKKLLAEMLAHPFFGSKPPKTTGREEFGEPFVRRMFASARRL
                     RLPDADIVATATALTAASIADAYERFVFPKLNQPGRKNLQVILGGGGAKNATLRRMLA
                     ARIAPAELLTQEDLGCSVAGKEALAFAILAHETLLGQPSNVPSATGARQPVVLGKIVP
                     AK"
     gene            complement(2209..2850)
                     /locus_tag="VG754_06985"
     CDS             complement(2209..2850)
                     /locus_tag="VG754_06985"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWF18996.1"
                     /translation="MVQYKPKTNFFTTLPRSFYGPSAEIVAAKLAGHLLIRNTPQGDC
                     GGIIIETEAYITGDPAAHSFRGQTKRNRAMWGPAGHSYVYFIYGNHWCFNVVCCPPGF
                     GEAVLIRAIEPTVGLETMRARRPVVKLRDLSNGPGKLCAAMDIDRRLDGVDLCDTESP
                     IFVAENPDLKETLKKYGPMVTTTRIGITQAAHMPFRFYLAGSHFVSKRAVGLA"
     gene            2999..3556
                     /locus_tag="VG754_06990"
     CDS             2999..3556
                     /locus_tag="VG754_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWF18997.1"
                     /translation="MNASPTEIWTIGHSTRSIGEFLGLLRENSINALVDVRRFAGSRR
                     HPQFSQVQFSSSLAEAGIKYFHLPELGGRRAPLPDSPNTAWRNAAFRGYADYMMTDEF
                     RGGMERLQEIAGKSRTAIMCAEALWWQCHRSLIADDLKARGWTVWNILGAGKVELHPF
                     TSAARLVNEKLSYRAAEREEELPLG"
     gene            complement(3514..4740)
                     /locus_tag="VG754_06995"
     CDS             complement(3514..4740)
                     /locus_tag="VG754_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWF18998.1"
                     /translation="MKPRSEGLPSQPAERPASVSENRAYPGRGSAAVVTVFLVVAALK
                     LGREVFLPLALATLCSFLLSPLVTRFRRWHLGRIPSVLIAVLIAFAVLGAMGTFMASQ
                     MGDLARKLPGYQENFQKKWQSVAQKGGGFFGRITGSAENLRKQLTPTNTNTQQQTGGQ
                     NKNEKPTPVEVKNPALSPLTVVRTVIGSILSALLMLFIVIVFVIFMLFERENLRKRLI
                     SLINGSTESTTELLDEASDRVSRYLLMQLVVNVTYAIPITVCLYFVGIPNPILWGVLS
                     GLLRYIPYIGPWIAMALPATMALAVDPGWDKPLLVFGLYGAVELIVANFVEPWLYGSS
                     TGITPLAVLFAALFWTWIWGPLGLLLSTPLTVCLVSFGRYLPSLGFLRILFGDEPGDA
                     KKNQPKGNSSSRSAAR"
     gene            complement(4737..5447)
                     /locus_tag="VG754_07000"
     CDS             complement(4737..5447)
                     /locus_tag="VG754_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HWF18999.1"
                     /translation="MTVTAQFQDRYEAGRFLATKLGHHAGNSSLVVLALPRGGVPVAY
                     EVAKALNAPLDIFLVRKLNMPGYEELSVGTVASGDVKILNDEAVHHLGVSQNLIDSLV
                     QERQQDLQRREKIYRGQRPPIEIEGRPVILVDDGLATGSNMRASVHALLERHPKSVTV
                     AVPIGSADTCQQMAREADEVVCAMTPDTFYSVGAWYSDFIQITDEEARQLLDHAAHER
                     RAKQAQRKNSASPEKVMS"
     gene            5617..5979
                     /locus_tag="VG754_07005"
     CDS             5617..5979
                     /locus_tag="VG754_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HWF19000.1"
                     /translation="MKPITALNEAELEETFSRASGPGGQNVNKVSTRVTLRHLPTSIS
                     VTVQDSRSQAMNRQLARERLLAALQQRERQAAAAARHQREQLRRQTAKRPRVAKERIL
                     AAKHHRAAVKKERRFRDE"
BASE COUNT         1394 a         1668 c         1673 g         1300 t
ORIGIN      
        1 ctcgacgttc aacgaggcca gttgttcaag ccacttgcgc aactggggca atagcgcgcg
       61 cgcttcttca cggctgtagt gtttgtgaaa tttttggctc atgcgctggt cgttgaatcc
      121 aaaacgactc ttgatcaacc atagatgaac acggataaag acggatggaa aggtaaaaac
      181 gagcgtttgc cttgcaggag cgcatctcac tattttgcac gtttctgggc tcttgcgaat
      241 tgtgttcatg ggccggcgca catcgctgtc cccttcactc cagccctccc aagggaccac
      301 gcaacgcgaa gcgttcgtct cctttgggaa actgacttgt ggcgggcttg ggaagtcatt
      361 gggctgggtg agggggagga aatgttcaat cactccgttg tgcaggaaac tgagatgcgc
      421 ccccttttct gacgcatccg cgaacttgaa gcgttatttc gcaggcacaa tttttcctaa
      481 aacgacgggc tggcgggccc cggttgcaga aggaacattg gagggctgac caagcaatgt
      541 ttcgtgggcg agaattgcaa atgcaagcgc ctccttgccg gcaacggaac agcccaggtc
      601 ttcctgcgtc agcaactccg cgggagcaat ccgcgcggcc agcatccggc gcagagtggc
      661 gttcttcgcg ccgccgccgc cgagaatcac ttgcaaattt tttcgcccgg gctgattcaa
      721 tttcgggaat acaaatcgtt cgtatgcgtc cgcgatgctg gcagcggtca atgcggtggc
      781 cgttgccaca atgtccgcgt ccggcaatct caatcgccgc gcggaagcaa acatgcgccg
      841 gacaaatggt tcaccaaatt cttcgcggcc agtggtcttc ggcggtttgc tgccaaaaaa
      901 tggatgcgcg agcatttccg ccagaagctt cttggaaact tttcctttcg cggcccattg
      961 tccgttccga tcataacgaa ctttgccgga acttaactcg ttcacgacgg cgtccatcac
     1021 catgttgccc ggcccggtgt cgaacgccat cacctcttgt aacgcggcgc gcggcggcag
     1081 gaaggtcaag ttgccaatgc cgccaatgtt ctggagaatg cgcggcctcg ccgaatcggt
     1141 ttgcaaagcc caatccacat aagtcaccag cggcgcgcct tgaccgcccg cggccatgtc
     1201 gcgcacgcga aaatctgcga cggtcgtgat gccggtgcgt tcggcgatga cgcagggttc
     1261 gccgatctga agcgtggaag gtgttttggc ccgtggcagg tgatgaatcg tctgcccgtg
     1321 cgacccgatt gccgcaatct gttccggctt caacccagcc tgccggatga ccgcgagcgc
     1381 ggcgcgcgca aaatgttctc ccatcaagaa attcagttcg caaatctccg cgactgttcc
     1441 gtgcagacag atatgcaaga tatgcttccg caataatggc gcgaaagctt gttgatggtg
     1501 cgcaatcaac cgcgctttca atcccctgcc cgtcccggag atttcggcga caacagcatc
     1561 gatgccgtcg gctgacgttc ccgacatcag tccgataaca tgatgactga ggcggccgga
     1621 acgagattga tttcgcatgg atgaaggcta atcaggccgg ccaacgcgga caagagcgta
     1681 acaatgattt caagaattca tgggcaaact cagtcggaac aattcagtct gaaaatgggg
     1741 aggaaggtat aatcgaggaa aacacaaaaa ctaaaaagtg atgcctttaa aggcgacact
     1801 ctaccgcatc gaaaatggcg cgatgcggta gagtgattac ttctttatct tttgtcaatg
     1861 aaatacttca catgaaatat ttcacacagg taattgtacg ggcgcatctt gccactgttc
     1921 ttgagaatgt acaggtatgg tttggaggac gctcgccctc accccggccc tcccccaaga
     1981 gggtgagcgc agtcacgttc aagaaccttt gatcagttcg gttgcaatca ccgcctgtca
     2041 aaaattattg aggcggagac tgaaaaccga ggacgatttc atttcaggtg gcggtaaatg
     2101 gttctccctc tccccggggt gcgggcgagc gtaaatccac gatgttcttt gccttgcacc
     2161 tgcaaaaacc cgctcgactg aaaaatagca aagaacgtag ttgctccctc acgccaaacc
     2221 aaccgcccgc ttggaaacga aatggctgcc tgcgagataa aatcggaacg gcatgtgcgc
     2281 ggcttgtgtg atgccgatgc gcgtcgtggt gaccatcggg ccatattttt tcagcgtttc
     2341 cttcaaatcc ggattttcag caacgaatat cggtgactca gtgtcgcaca aatccactcc
     2401 atctaaacgg cggtcaatgt ccattgcagc gcataatttt cccgggccgt tggagaggtc
     2461 gcgaagcttt accacggggc ggcgcgcgcg catcgtttcc aaaccgactg tcggctcgat
     2521 ggcgcggatc aacaccgctt caccaaagcc cggcggacag caaacgacgt tgaagcacca
     2581 atgattgcca tagatgaaat aaacgtaact atgacccgcc ggaccccaca tggcgcggtt
     2641 gcgtttcgtt tggccgcgaa aagaatgcgc agcgggatcg ccggtgatgt aggcttcggt
     2701 ttcgatgatg atgccgccgc aatcaccttg cggagtgttc cgaatcagca gatgaccggc
     2761 taatttggcc gccacgattt cggcggaggg tccgtaaaaa gaacgcggca aagtcgtgaa
     2821 gaaatttgtt tttggcttat attgaaccat gctgattagc actggagcgc ggctgtgcgc
     2881 gaagcgccag ccgcagcaaa cgactgaact cccgagtgtt ccaagctgcc gcggctggtc
     2941 ctgcggacat agccgcgctc cgttctctat ttgagatgat ttgtaccaca atgcgcgcat
     3001 gaacgccagc ccgactgaaa tctggacgat tggacattcg acgcggagca tcggcgaatt
     3061 tctcggtttg ctccgggaaa acagcatcaa cgctctcgtt gacgtgcggc ggttcgccgg
     3121 ttcgcgccgt cacccgcaat tcagccaggt ccagttttcg tcatcactcg ccgaagccgg
     3181 gatcaaatat ttccatctgc cggaactggg tggacgccgt gcgccgcttc ccgattcacc
     3241 caatacggcg tggcggaatg cggcttttcg cggctacgcc gattacatga tgaccgatga
     3301 atttcgtggt gggatggagc gcttgcagga gattgccgga aagtcgcgca cggcgatcat
     3361 gtgcgccgag gcgctctggt ggcaatgtca tcgttccctt atcgcggacg atttgaaagc
     3421 gcgcggatgg acggtgtgga acatcttggg cgcgggaaaa gttgagttgc atccgttcac
     3481 ttcggcggcg cggcttgtga atgaaaagct cagttatcga gcggcagagc gcgaagagga
     3541 attgccttta ggttgatttt tctttgcatc cccgggttca tctccgaaaa gaatgcggag
     3601 aaaacccagg ctcggcagat aacgtccgaa tgaaaccagg cagaccgtca gaggcgtgga
     3661 aagcaaaaga cccaatggcc cccagatcca cgtccagaaa agggccgcga acaaaactgc
     3721 caatggcgtg atgccagtcg aggagccata caaccagggt tcgacaaagt tcgctacgat
     3781 aagttccact gccccataca atccaaaaac cagcaatggc ttgtcccaac ccgggtccac
     3841 agccagcgcc atggttgcag gcaaagccat cgcaatccac ggcccgatgt aaggaatgta
     3901 gcggagcaat cccgacagca cgccccataa aattggattg ggaatgccca cgaaatataa
     3961 gcataccgta atcggaatcg cataggtgac attgacgacc agttgcatga gaaggtagcg
     4021 gctcacccgg tcgctggctt cgtccagcag ttcagtagtt gattcagtcg atccattgat
     4081 gagactgatc agtctctttc gcaaattttc ccgttcaaag agcatgaaaa ttacgaaaac
     4141 aatcacgata aacagcatca ggagcgcact gaggatggac ccaatgacgg tccgaacgac
     4201 tgtcaacggc gacaaggcag gattcttgac ttccaccggg gtcggctttt cgttcttgtt
     4261 ttggccgccg gtttgctgct gcgtgtttgt gtttgttgga gtcaattgtt tgcgcaggtt
     4321 ttcggccgaa cctgtgatgc gaccgaaaaa cccgcctcct ttttgcgcga cggactgcca
     4381 ttttttttgg aagttttcct gatagccggg cagcttgcgc gccaaatcgc ccatctggct
     4441 ggccatgaat gttcccatcg cgcccagaac cgcgaaagca atcaaaacgg cgatcaacac
     4501 ggaagggatg cgtcccagat gccaccgccg aaatcgggtc accaatggcg aaagcaaaaa
     4561 actgcagaga gttgccagcg ccaacggcag aaatacttcg cggcccaatt taagagcggc
     4621 cacgaccaga aatacagtca ccaccgctgc gcttccacga ccgggatacg cccggttctc
     4681 tgaaaccgat gcgggtcttt ccgctggttg cgacggcagt ccctcagagc ggggtttcat
     4741 gacatgactt tttcgggact cgcagagttt ttcctttgcg cctgtttcgc gcggcgctca
     4801 tgggccgcat ggtcgagcaa ttgacgcgct tcttcatcgg tgatctggat gaaatcggaa
     4861 taccaggcgc ccacggaata gaacgtgtcc ggcgtcatcg cgcaaacaac ctcgtccgct
     4921 tccctcgcca tctgctggca ggtatcggct gagccgatgg gcacggcaac ggtcactgac
     4981 ttgggatgcc tttcgagcaa cgcgtgcact gatgcgcgca tgttggaccc ggtggccagt
     5041 ccatcgtcca ccaggatcac cggtcggccc tcgatctcga tgggcggtct ttggccgcgg
     5101 tagatttttt cgcgacgctg caaatcttgt tggcgttcct ggaccagcga gtcaatgagg
     5161 ttttgggaaa cgcccaggtg gtgaacggct tcgtcattca atatcttgac gtctcctgac
     5221 gcaactgttc ccacagaaag ctcctcgtag cccggcatgt tgagttttct caccaaaaat
     5281 atgtcgaggg gcgcgttcaa agccttggcg acttcatagg ccaccggcac gccaccgcgc
     5341 ggcaacgcca gcaccacaag cgatgaattt ccggcgtgat ggccaagttt ggtcgccaga
     5401 aaacgcccgg cttcgtagcg gtcttgaaat tgtgctgtga cagtcatata cgcgcagatt
     5461 gcatcttcta atagacggtg catcccggtg cgatggaatg cacagtaacc ctcctttggt
     5521 gttggaaaaa taagtaggaa actttggaaa ccgccaaatc gttcttgccc ggcaatcttc
     5581 gcgtggcaca taattcgatg cccgaaagcg gcacaaatga aaccgatcac tgccctgaat
     5641 gaagctgagt tggaggaaac tttcagccgc gcctccggcc cgggcggtca gaacgtcaac
     5701 aaggtttcca cccgcgtcac gctccgccat ttgccgacca gcatcagcgt cactgtccag
     5761 gattcgcgtt cacaggcgat gaatcgacag cttgcgcgtg aacgattgct ggctgcattg
     5821 caacaacgcg aacgccaggc cgccgccgcc gcccgacatc aacgcgagca attgcgccgt
     5881 cagacggcaa aacgtccgcg tgttgccaag gaacggatac tggccgccaa acatcatcgc
     5941 gctgcggtca agaaggagcg gcgtttcaga gatgagtgag atcgagggaa tggagtgatg
     6001 agcaatattg agcgcgcctg taattcaata gattt
//
LOCUS       DASZMM010000292         4916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3969848, whole genome shotgun sequence.
ACCESSION   DASZMM010000292 DASZMM010000000
VERSION     DASZMM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3969848"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(846..935)
                     /locus_tag="VG754_07010"
     tRNA            complement(846..935)
                     /locus_tag="VG754_07010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(899..901),aa:Ser,seq:gct)
     gene            complement(1012..2472)
                     /locus_tag="VG754_07015"
     CDS             complement(1012..2472)
                     /locus_tag="VG754_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19001.1"
                     /translation="MKLLLKILGRAGDFSDVQPLLALFLGGALLAVFLAVIFQSRAKP
                     AESSSLLWTLYMKAGTFLWALFLVGFLVSGLSVLRSYLHQTVAKFQRSHGRVTEANYN
                     AVQTIWGAEQSQDELHMDLYYEEEVTERIESEDLTKPAILRKKIVRHDVSANPFLSAR
                     HEVTLRQNPRKKGSALYGGYETACRFSWRLQNPADRQLNADIKFPLPAQGAVYNDLYA
                     TLNGKDILPLMEIKDGALNLPRIVKPGEQLDIKMGFKSRGMSSWYFQVKEPREIRDFM
                     LTLTLPDLSKAHLNNPEGCMTPTDVKPTADGLGSVLSYRLDHAISSKGMGIALPTPPQ
                     PGEITNAVLGETEGSWLLIIALLVLGMTITSSRNAVLLSLLFGAATALGYGLLGDFSD
                     LLFGFWGTAALVLAPAFILLAWFLRKLLPGINGKLMAGQLLLYGILLPCVAGLDSERQ
                     SLYLNICAGLFLAFAAWLLLKPRGTEPSAEMAAASA"
     gene            2750..4093
                     /locus_tag="VG754_07020"
     CDS             2750..4093
                     /locus_tag="VG754_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19002.1"
                     /translation="MKTAILHWSLFPVLSFAIATAMAQSASDAVMSPASGEPQVIHLD
                     VHTKLTLLGTSYGYHHMAPGYEKLSTANWIYTAPNTTVVWIEEEREPNTRSTYELLVS
                     DRANTGCVNIEPRQGSFVKPGVSLQSFVLTAFPRWDKETILRVRPHRGSPSKEQFVLT
                     NPVPETIAEWTPDPLPATKSAGDLEVTLTKLIAGAPMPYREGRRRTATNDPANQCVHF
                     DFDFRQNGQSTTNWQAWPVETTDAAGNWSRGLIYSYPKNGINPSYPDRIHPSFPPEND
                     GHYFQPGLWPDTPWKVKLEFIQRFNFSDDEMVTFTNIPVKPGSQKDHDDQWSAWDVGK
                     TNFPFSIIPGTVNGVHLKLLPPLLLTNRWQSSEMDVSIIIGADPGFNPKGMNLSVVSA
                     TDDQGRDLWHFEQPAWAGHYSMEFARVHDDIKSLNLKLALHKSRFVEFTVKPARQ"
     gene            4251..4664
                     /locus_tag="VG754_07025"
     CDS             4251..4664
                     /locus_tag="VG754_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19003.1"
                     /translation="MKRAIGFLFVITACLFIAWAEKPLNPDSYFAYPRAAIVIESGKT
                     KSEAECSKTYSVPTKQDMEPHFRSGTEFEGGISVDWEFVRQTEFGDVYLIAIDRPGKP
                     QNVVPVLFKGKAQTIISTNDLSVMILPEYHKKKSG"
     gene            complement(4700..>4916)
                     /locus_tag="VG754_07030"
     CDS             complement(4700..>4916)
                     /locus_tag="VG754_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HWF19004.1"
                     /translation="FYVAITRAMESLTVSHCAGRKKYGQVMPCHPSPFLKELPPELVE
                     HADEKAKQPVTVDAGKSRFDALRAALD"
BASE COUNT         1138 a         1363 c         1339 g         1076 t
ORIGIN      
        1 gtccggggtg ggcggcgctt tgtccaatca tctcctttcc gatcgacccg ggcctcccaa
       61 cccctttcct gccatctgct aaagccaccg caaaactccc gccctcatcc aagcagccgc
      121 tataccggac actattggtc accgacccgc gctccggggc agtgcgccga tgcgcccctt
      181 ggttttgggc acatccacac gctctcccga attgttgccg atgctgggtg cgatttgctg
      241 ttgcgttaac cgtgggaagc ggagcgcggc tcgatgagcc gcagcaacgc gcgatgaacc
      301 ggacacgcca gaccaattca tgcggcctaa ccttcccaca ttgctgcggg tcaccgaccc
      361 cgcgctccgg ggcagtgcgc cgatgcgcct cttggttttg ggcacatcca cacgctctct
      421 cgaattgttg ccgatgctgg gtgcgattgc tgttgcgtta accgtgggaa gcggagcgcg
      481 gctcgatgag ccgcagcaac gcgcgatgaa ccggacacgc cagaccaatt cgtgcggcct
      541 aaccttccca cattgctgca ggtcaccgac ccgcgctccg gggcagtgcg ccgatgcgcc
      601 ccttggtttt gggcacatcc acacgctctc ccgaattgtt gccgatgctg ggtgcgattg
      661 ctgttgcgtt aaccgtggga agcggagcgc ggctcgatga gccgcagcaa cgcaccagaa
      721 ccagaacacg cacgactaat tcggacacag ccggtattcc cacatttctg cgggtcatct
      781 tccaaccggt tgttgtactt tgatgctcgc tcgtggataa tggaggcagc gatccttgat
      841 ttaattggcg gagagagagg gattcgaacc ctcggaagga ttactccttc acaggtttag
      901 caaacctgcg ctttaggcca ctcagccatc tctccgctat cggttgcggg attcatactg
      961 ttacccgcgg cacggacatt ggcaagccga tttgtgcatc tcgtgcatgg ttcaggctga
     1021 tgccgccgcc atttctgcgc tcggttccgt gccgcgcggt ttcaatagca accatgcggc
     1081 gaatgccagg aacaatcccg cgcaaatgtt caggtaaagc gattgccgtt cggaatccaa
     1141 gcctgcgaca catggcaaaa ggatgccgta aagcaacaat tggccggcca tcagtttgcc
     1201 gttgatgccg ggaagcagtt tgcgcaaaaa ccacgcgagc aggatgaacg ccggggccag
     1261 caccaatgcc gccgttcccc aaaatccaaa cagcaaatcg ctgaagtcgc ccagcaatcc
     1321 gtaacccaac gccgtcgcgg cgccaaataa aagactcagc aacacggcgt tgcgcgatga
     1381 agtgatggtc attcccagga cgagcaacgc aatgatgaga agccagcttc cctcagtttc
     1441 gccaagcacg gcgttggtga tttcgccggg ttgcggcggc gttggcaaag cgattcccat
     1501 ccccttgctc gaaatcgcat ggtccagccg ataggacagc actgagccta aaccatccgc
     1561 ggttggcttg acgtcggtgg gagtcatgca gccttcggga ttgttgaggt gcgctttgga
     1621 caaatccggc agcgtcaatg taagcataaa atcgcgaatt tctcgcggct ctttgacttg
     1681 gaaataccaa gatgacattc cgcggctttt gaaacccatc ttgatgtcca attgctcgcc
     1741 gggtttcacg atgcgcggca gattcagcgc gccatccttg atttccatga gcgggaggat
     1801 gtccttcccg ttcaacgtgg cgtaaaggtc gttgtaaacc gcgccttggg caggcaacgg
     1861 aaacttgatg tccgcgttca actggcggtc ggcggggttt tgcaatcgcc aggaaaagcg
     1921 gcaggcagtt tcatagccgc cgtaaagcgc cgaacctttt ttgcgcgggt tttggcgcaa
     1981 cgtgacttca tgacgcgcgg aaaggaacgg attggcgctt acgtcgtgcc gcacgatttt
     2041 cttgcgcaga atggcgggtt tggtgaggtc ctccgattcg atgcgttcgg tgacttcctc
     2101 ctcgtaataa aggtccatgt gcaattcatc ctggctttgt tccgcgcccc agatggtctg
     2161 gacggcgttg taattcgcct cggtgacacg cccatggctg cgctggaact tggcgacggt
     2221 ctggtgcagg taggaacgca gcaccgaaag tccgctcacc aaaaacccca ccaggaacaa
     2281 cgcccaaagg aatgttcccg ccttcatgta aagcgtccaa agaaggcttg aagattccgc
     2341 tggtttggcg cgggattgaa aaatgacggc gaggaaaacg gcgagcaatg cgccgccgag
     2401 aaacagcgcg agcaacggtt gaacatcgga aaagtcgcca gcgcgaccca gtattttgag
     2461 caatagtttc atggcatttg gtttgttggt tcattctgtt tggtgccatg gcgcggccga
     2521 aaattggaac aagaggaatc aaggtcacac cacggcgatc agcttgcacc ttgctctgtt
     2581 gaggatggca ggacgggatg caggactctt tacgcgcact cgaacaagtc caaggacgca
     2641 ggcgcattac aaacctgaat caaaaatttg tccacaactt tttgaaaaaa atcgttgttt
     2701 gactctgaaa cgcgacgatg cttaccttgc acaaagtggg caaaacatta tgaagacagc
     2761 catcctgcac tggagccttt ttccagtgtt aagcttcgcc atcgcaaccg cgatggccca
     2821 aagcgcgagc gatgccgtca tgtctcccgc ctccggggaa ccgcaagtca tccatttgga
     2881 tgtccacacg aagctgacat tgctgggcac gagttacggg tatcaccaca tggcgccggg
     2941 ttatgaaaag ctcagcacgg ccaactggat ttacaccgcc ccgaacacca ccgtggtctg
     3001 gattgaagag gagcgcgaac cgaacacgag gtcaacctac gaattgctgg tttctgatcg
     3061 ggcgaacacg ggttgcgtca acatcgagcc gcgacaaggg tcatttgtaa agccgggcgt
     3121 gtcgctgcaa agtttcgtgc tgactgcgtt cccgcgttgg gacaaagaaa caattctgcg
     3181 ggtcaggccg cataggggca gcccctccaa agagcagttt gtcctcacca atcctgtgcc
     3241 tgaaacgatt gcggagtgga cgccggaccc attgccagca acgaaatccg ccggcgatct
     3301 ggaggtgacg ctcaccaagc tgattgccgg tgcgccgatg ccttatcgtg agggccgccg
     3361 ccgtacggcg accaatgatc cggcgaatca atgcgtccat ttcgattttg attttcggca
     3421 aaacggccag tccacgacca actggcaagc ctggccggtg gagacgaccg atgcagccgg
     3481 caattggtct cgcggtttga tctacagcta ccctaaaaac ggcattaacc ccagctaccc
     3541 ggatcgcatc catccctctt ttccgccgga gaacgacggc cattatttcc agccggggtt
     3601 gtggccggat acaccgtgga aggtgaaact ggaattcatc caacggttta atttcagcga
     3661 tgatgaaatg gtgacgttca ccaatattcc cgtgaaaccc ggcagccaga aggaccatga
     3721 tgaccaatgg agcgcgtggg atgttggcaa aaccaatttt ccattttcca tcatcccggg
     3781 cacggtcaat ggagtgcatt tgaaattgct tccgccgttg ctcctcacta atcgctggca
     3841 aagcagcgag atggacgtca gcatcatcat cggggccgac cccggtttca accccaaagg
     3901 catgaacctg tccgtggtgt cggccaccga tgatcaagga cgcgacctct ggcatttcga
     3961 gcaaccagct tgggctggcc attattcaat ggagttcgcg cgtgttcacg atgacatcaa
     4021 atcgctgaac ttgaaattgg ccttgcacaa aagccggttc gtggaattca ccgtcaagcc
     4081 ggccagacag tgaatgtgaa ccacaaaaat caagcgcctc aatccaacat cgcgcgcaac
     4141 gcatcaaagg atctttcttg gaagccatga tttgaaaaaa tgttgaattc gccatgattt
     4201 ttcattacaa ttattggcga atcaatggag ccactaattt ctaaatcact atgaaacgag
     4261 ctatcggatt tctttttgta atcacggctt gtctgttcat tgcatgggca gaaaagccgc
     4321 tgaatccgga cagttacttt gcgtatcctc gcgccgcaat cgtaatagag tccggaaaga
     4381 caaaatcgga ggccgagtgt tccaaaactt atagcgtccc caccaagcaa gatatggaac
     4441 ctcatttccg gtccggaacg gaattcgaag gtgggattag cgtagattgg gagtttgttc
     4501 gccagacaga atttggcgat gtttatctca ttgcgatcga ccggcctggc aaaccccaaa
     4561 atgttgtgcc ggtcctcttt aagggaaagg cccagacaat catctctaca aatgatttaa
     4621 gcgtgatgat attgcctgaa taccataaaa agaaatcagg ttaacaaaga tgtggccttc
     4681 ttaataatgg cttcttcatt caatccaacg ccgcccgcaa ggcatcaaaa cggcttttgc
     4741 cggcatcgac agtgacgggt tgcttcgctt tttcatcggc gtgttcgacc aactccggcg
     4801 ggagttcttt gagaaacggc gagggatggc agggcatgac ttgtccgtat tttttgcgcc
     4861 cggcgcaatg gctgacggtc aggctttcca tggcgcgggt gatggcgacg tagaaa
//
LOCUS       DASZMM010000293         6879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3136771, whole genome shotgun sequence.
ACCESSION   DASZMM010000293 DASZMM010000000
VERSION     DASZMM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3136771"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VG754_07035"
     CDS             complement(<1..189)
                     /locus_tag="VG754_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19005.1"
                     /translation="MKSSRAEARKTLVRRVAAPLVEIFGLSWRLAFATVLFILAVLVF
                     AVYWSIHTSPPRTIYISSG"
     gene            319..1725
                     /gene="pyk"
                     /locus_tag="VG754_07040"
     CDS             319..1725
                     /gene="pyk"
                     /locus_tag="VG754_07040"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWF19006.1"
                     /translation="MRKTKIIATLGPATDSPEMLGKLMDAGLNIFRLNMSHATHDWTR
                     RVVRDIRALAAERSRVVGILMDTQGPAIRTGDLPVPLHLRPGEHFTFTVRGAKSEEIN
                     SVDVNYAEFINDIHVGDVVLVDNGAIQMKVLAKHGNKVECEILTDGKLGSRRHINLPG
                     VKVSLPAMTEKDIADVHLGLELGVDYIALSFVRTARDVRELKKLVTRAKSHRPLIVAK
                     IEDQQAVKNLEAILMDSDAIMVARGDLGIEVPYEELPIIQRRIVKMCLHVGRPVIVAT
                     HMLESMIQSPMPTRAEVTDVANAVFEQADAIMLSGETTVGKYPVKCVEVLDRIAQRTE
                     QSGGAGYAEQANLTDPREKLAKSAIVMANELRAQAIVTLIRHGSMTRYLSWLRPRYSL
                     IYAAGPSEQIACQLALNRGVIPFILPLDPIDLDGNITRALKTLVEKKFLRKGNTAVVV
                     SSINADNEILDAIQMRVV"
     gene            2008..3219
                     /locus_tag="VG754_07045"
     CDS             2008..3219
                     /locus_tag="VG754_07045"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWF19007.1"
                     /translation="MKLRHKTNYMPGRAEGRKPEEKDAAPKEDKKIRRHRRWRRFGIV
                     LLVLAVLLGIFRAFLPLMVRDYVNRTLDQNPLYSGQIGTVGIHLWRGAYSIDDVRLNK
                     TSGNVPVPLFAAKRVDFSVQWKALVHKKVVTQVVMDQPELNFVDSPSESESQSGGGGP
                     WLQMIQQLSPFKVNSAQLHNGSVHFRTYKSQKPVDVYVSQVQATVDNLGNIRNDTTPL
                     VATVQASGLVMDQAQLELKMTLDPFSYKPTFHLGLRLLGLDVTKINDLALTYGKFDFK
                     RGWFDLVIETDCEEGQITGYVKPLFRNLKVFSLRQDIKEDNVLEFFWQALVGAATTLF
                     KNQPHDQFGTLIPFTGDVTESTNTDLLATIGNILRNAFVRAYLPRLQNSEQAIDGMTF
                     SPPDLSSPNSP"
     gene            3228..3953
                     /locus_tag="VG754_07050"
     CDS             3228..3953
                     /locus_tag="VG754_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HWF19008.1"
                     /translation="MNTNPNHTYETRRSLRMAWAVRGLTLSLLAAFVAIGAGCHSKSP
                     EKQNKDFFTSGSREADQRASQTMAKSEQLAGSGEGAGEREKGAVGNNSGTNGAAVAKG
                     KESLFERLGGDAGITALVEDFLPRAMQDPRVNWDRNGVKRGGFGFTSSESVTWKATPQ
                     NVATLKKHLVEFLELATGGPSHYEGRDMKSAHAGMRISNPEFDAVVGDLKVSLDRLKI
                     ANTEQKELLAILESTRPEIVTQR"
     gene            complement(4097..5554)
                     /locus_tag="VG754_07055"
     CDS             complement(4097..5554)
                     /locus_tag="VG754_07055"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWF19009.1"
                     /translation="MNYSLIILEAAVLALGLGILLADLWTPSVEQKRQLGWIAAIGLG
                     LILLFSFKFSGGDPQYAFGKMYVMDSLALFFKRFFLIATILVLVMSVEFADQIETGIS
                     EFYALILFALVGMMFAASANDFSMLFVSIELITVTFYVLTSFQRGRLKSLEAGVKYLI
                     LGALSSAFMVYGIALVFGTTGKLNFQGVAAMAAQLEGNKLFLLGILLTLVGLGFKIAA
                     FPVQMWAPDVYQGSPTPTTAFLAIGSKAAGFALLLRVLFDAVPDITAHWTKLLIVISM
                     VTILYGNLCAIPQRNLKRLMGYSSIANAGYMLMGVAAFGKDGQSAVLYYLAGYLFTVL
                     GAFTVICLVTRRLDSDDISALAGLNQRSPLLAATMTLAMVSLAGIPPLAGFFGKFLLL
                     KAVVEQGAKQPAYYWLVAVALAGVVISIYYYFGVVRAIYWSKDTPDLSPIKISMPAKV
                     AIYVCVIGMLFLGLFPDAAVNWADKAAEALQMVKL"
     gene            complement(5564..>6879)
                     /locus_tag="VG754_07060"
     CDS             complement(5564..>6879)
                     /locus_tag="VG754_07060"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWF19010.1"
                     /translation="HYHVGVDGLNVGLILMGAIVAFAAACCAWEIRDREKEFYILLLV
                     MTGGILGAFASLDLFFFYFFHELALVPTFIMIGVWGRGKNKNYATFQITMYLSIGALV
                     ALIGLIVLYIQAGAHTFDIVKLTEHIQQNPLSLHDQNFIFPLLLFGFGILISLWPFHT
                     WAPLGYGSAPTPTAMLHAGVLKKFGLYGLIRLAVPLLPQAAAHWAHIIAWLCLGNIVY
                     CGLVAMRQKNFNWLIGYSSVAHMGFVFLGIASLNLLGITGAVLVMVAHGFLAALTFGL
                     SGYIYHQTGTMEMAKLGGLLRRMRFVGAVLIMAMFAGCGLPGFANFAGEVTVFFGAWK
                     VPAFHFIIGLAVWGALVIGAVYMMRGIRNVLHGPLPEPLMAVAESTWPLRKVPYLALL
                     ISLLVFGFFPSLLSDKIEPSAKKILAMMNGEKPAAAETTAMATVK"
BASE COUNT         1691 a         2068 c         1746 g         1374 t
ORIGIN      
        1 tccgctggag atatagatgg tcctgggcgg cgaggtatgg atggaccagt acacggcaaa
       61 tacgagcacg gccaggatga aaagcaccgt ggcgaatgcc agccgccagc ttaatccaaa
      121 aatttccacc agcggcgcag ccacccgcct gaccagcgtc tttctcgctt ctgccctgct
      181 cgatttcatt ttttccaatc tgtttccggt tttcaatcaa cttgtccaag tgagaggctt
      241 gttataagcc gaacctcgat tttgcccaat gtcaaacttt gaactttaaa ctttaaactt
      301 tccacccgag actcagccat gcggaaaacg aaaatcatcg ccacgctcgg gcccgcaacc
      361 gattcgccgg aaatgctcgg caagctcatg gacgccgggc tgaacatttt ccggctcaat
      421 atgtcccacg ccacccacga ttggacacgc cgggtggtcc gtgacattcg cgctctcgcc
      481 gccgaacgca gccgggtcgt cggcattctt atggacacgc aagggcccgc aatccgcacc
      541 ggcgatcttc ccgtgccgct ccatcttcgc ccgggagagc atttcacctt caccgtccgc
      601 ggcgcgaaaa gcgaggaaat caattccgtg gatgtcaatt acgcggagtt cattaacgac
      661 attcacgtcg gcgacgtcgt gctcgtggac aacggcgcga tccagatgaa agtcctcgcc
      721 aagcacggca acaaagtcga atgcgaaatc ctcaccgacg gcaagcttgg cagccgacgg
      781 cacatcaatc ttcccggcgt aaaagtcagc ctgcccgcga tgaccgagaa ggacattgcc
      841 gacgttcatc ttgggcttga acttggcgtg gattacatcg cgctctcgtt tgtccgcacg
      901 gcccgcgacg tgcgcgaact caaaaaactt gtcacgcgtg ccaaatcgca tcgcccgctc
      961 atcgtcgcca aaattgaaga ccagcaggcg gtcaaaaatt tggaagccat cctcatggac
     1021 tcggacgcca tcatggttgc gcgcggcgat cttgggattg aagttccgta cgaagagctt
     1081 cccatcatcc aacggcgcat cgtgaagatg tgcctgcatg tcgggcgtcc tgtcatcgtc
     1141 gccacgcaca tgctcgaaag catgatccaa tcgcccatgc ccactcgcgc ggaagtcact
     1201 gatgtcgcca atgcggtttt cgagcaggcc gatgccatca tgctcagcgg cgaaaccacc
     1261 gtgggaaaat atccggtgaa atgcgtcgaa gtcctggacc gcatcgccca acgcactgaa
     1321 caaagcggcg gcgcgggtta tgccgagcag gccaatctga ccgacccgcg tgaaaagctt
     1381 gccaaaagcg ccatcgtcat ggccaatgaa cttcgggcgc aagccatcgt cacgctcatc
     1441 cggcacggca gcatgacgcg gtatctttcg tggttgcgcc cgcgttattc gctcatttac
     1501 gccgccggcc cgagcgaaca aatcgcctgc cagcttgcgc tgaaccgcgg tgtcatcccc
     1561 ttcatcctgc ccctcgatcc cattgacctt gacggcaaca tcacgcgcgc gctgaaaacc
     1621 ttggtcgaga aaaaatttct gcgcaaagga aataccgcgg tggttgtcag ttccatcaac
     1681 gccgacaatg aaatccttga tgccatccag atgcgggtgg tgtgatttgt tttcgatatc
     1741 ttcttggcgg gttaactcgt ttgtcctaga aatcggcact cgtttgcctt aaaatgaaaa
     1801 cggagcgcgg ctgtgtccgc aggaccagcc gcagcagctt ggaaactcag ggtctttgtg
     1861 cttgctgcgg ccggcgcttc gcgcacagcc gcactccact attttgagat aaagtttgta
     1921 gcccgttttt ctacgcaaca tgccccattg ctgaatgccg cggggtgaat tactgattat
     1981 tattcaggaa gggagggctt tgttgtcatg aaactcaggc ataaaaccaa ttacatgcct
     2041 ggacgggccg aggggcgcaa accggaggaa aaggacgcgg ccccgaaaga agacaaaaaa
     2101 atccgcagac acaggcgttg gcggcggttt gggattgttt tgctcgtgct ggccgttttg
     2161 cttggcatct tccgcgcttt tcttccatta atggtccggg attatgtgaa ccgcaccctg
     2221 gaccaaaacc ctctttacag cggacaaatc ggcacggtcg gcattcacct ctggcgcggc
     2281 gcttacagca ttgacgatgt tcgcctcaac aaaacctcgg gcaatgttcc tgtcccgcta
     2341 ttcgcggcca aacgggtgga tttttccgtc cagtggaaag cgttggtcca caaaaaagtc
     2401 gtgacgcaag tcgtgatgga ccagccggaa ttgaactttg tggattctcc aagcgagagc
     2461 gaatcccaaa gcggcggcgg cgggccgtgg ctgcagatga tccagcaact ctctcctttc
     2521 aaggtgaaca gcgcgcaatt gcacaacggc tcggttcatt ttcgcactta caagtctcag
     2581 aagccggtgg atgtctatgt ctcccaagtg caggcgacgg ttgacaacct gggcaacatc
     2641 cgcaacgaca ccacgccgct ggtcgccacg gtgcaggcga gcggtttggt catggaccag
     2701 gcccagctcg aattgaagat gacgctcgac ccgttctcct acaaaccgac gtttcatctt
     2761 ggccttcgtt tgctcgggct ggatgtcacg aagatcaacg acctcgctct cacttacggc
     2821 aaatttgatt tcaaacgcgg ctggttcgac ctcgtcatcg aaacagattg cgaggaaggc
     2881 cagatcaccg gttatgtgaa accgctgttc cgaaatttga aggtgttcag tttgcgccag
     2941 gacatcaagg aagacaatgt tctcgaattt ttctggcagg cattggtcgg cgcggccacc
     3001 acccttttta aaaatcaacc gcatgatcaa ttcggcacgc tcatcccgtt caccggcgat
     3061 gtgaccgaaa gcaccaacac ggacctgctg gcaaccattg gaaacatcct gcgcaacgcg
     3121 ttcgtccgcg cttatctgcc gcgtctgcaa aacagcgagc aggcgatcga cggcatgaca
     3181 ttctcgccgc cggacttgtc ctcgccaaat tcaccatgat tactcttatg aacacaaacc
     3241 caaatcatac ttatgaaacc cgtcgttcgc ttcgcatggc ctgggccgtg cgcggattga
     3301 cactctcatt gcttgccgcg tttgttgcta tcggagccgg ttgccacagc aagtctcccg
     3361 aaaagcaaaa caaggacttc ttcacctccg gcagccgcga agctgaccaa cgggccagcc
     3421 aaacgatggc caagtccgag caactcgccg gatcgggtga aggcgcaggc gaacgggaga
     3481 aaggcgccgt cggaaataat agcggcacaa atggggcagc cgtggccaag ggcaaagaat
     3541 cactgttcga gcggctcggc ggtgacgcgg gcattaccgc gctggtggaa gattttctcc
     3601 cgcgcgccat gcaggacccg cgcgtgaatt gggaccgcaa tggcgtcaaa cgcggcggtt
     3661 ttggttttac cagcagcgaa tcagtcactt ggaaggccac gccgcagaat gttgcgaccc
     3721 tcaaaaaaca tctggtggaa tttcttgaat tggccaccgg cggcccgtcc cattacgagg
     3781 gcagagacat gaagtctgcg cacgctggca tgcgcatttc caatccggaa tttgacgcag
     3841 tcgtcggcga tctcaaagtc tcgcttgacc ggcttaagat cgcgaacacc gaacaaaagg
     3901 agttgctggc catccttgaa agcacccggc cggaaatcgt cacgcaacgg tagaggagca
     3961 ataaggtttg gccactgatt gaacacggaa caaagacgga ttcaagccat tttgattcca
     4021 aagaattgct ctcaatagca gcggtgtgac tgggaaagaa tcggtctttt ccatccgtgt
     4081 ttaatctgtg gcgtaactag agcttaacca tctgcaaagc ctcggcggct ttgtcggccc
     4141 agttcactgc ggcgtccggg aacaatccca aaaatagcat tccgatcacg cagacgtaaa
     4201 tcgcgacttt ggcaggcatt gaaattttta ttggcgacaa atccggtgtg tccttcgacc
     4261 agtaaatcgc ccgcaccacg ccgaaataat aataaatcga aatcaccacg ccagccagcg
     4321 ccaccgccac cagccagtaa tacgccggtt gcttcgcgcc ttgttccacg accgctttga
     4381 gcaaaaggaa tttgccaaaa aatcctgcca gcggcggaat tcccgccaac gacaccatcg
     4441 cgagcgtcat ggtcgccgcc agcaacggcg agcgttgatt cagtcccgcc aatgccgaaa
     4501 tgtcatcgct atccaaccgt cgcgtcacca gacaaatgac cgtgaacgcg cccaggaccg
     4561 tgaacaaata acccgccaga tagtacaaca ccgcggattg cccgtccttc ccaaatgccg
     4621 ccacgcccat cagcatgtag cccgcgtttg cgatgctcga atagcccatc agccgtttca
     4681 aattccgctg cggaatcgcg cacaaatttc cgtaaaggat tgtgaccatc gaaatgacga
     4741 tcaacaactt cgtccaatgc gccgtgatgt ccggcaccgc atcgaacaac acccgcagca
     4801 acagcgcgaa acccgcggcc ttcgaaccaa ttgccaggaa cgccgtcgtc ggcgtgggtg
     4861 aaccttgata cacgtcggga gcccacatct gcaccgggaa cgctgcgatc ttgaatccca
     4921 gtcccaccag cgtcagaaga atgccgagca aaaacagttt gtttccttcc aattgcgccg
     4981 ccatcgccgc cacgccttga aaattcaatt ttcctgtggt tccaaacacc agcgcgatcc
     5041 catacaccat gaacgccgac gacaacgcgc ccaggatgag atatttcacg cccgcttcga
     5101 gcgatttgag acggccgcgt tgaaagctcg tgaggacgta aaatgtcacg gtgatcaatt
     5161 cgatggacac gaacaacatg ctgaaatcgt tcgccgacgc tgcaaacatc atccccacca
     5221 gcgcgaacaa aatcagcgcg taaaactcgg agatgccagt ttcgatctgg tcggcgaact
     5281 ccaccgacat cacgagcact aatatcgtcg caatcaggaa aaatcgtttg aagaacaggg
     5341 ccagcgaatc catcacgtac attttcccaa aagcatattg cgggtcgcct ccagaaaatt
     5401 tgaagctgaa caacagtatc aggcccagtc caatggcggc gatccatcca agctggcgct
     5461 tttgctcgac cgatggcgtc cacaggtctg cgagcaggat tcccagcccc agcgccaaca
     5521 ccgccgcttc caaaatgatc aacgagtaat tcatccgatt ccgttatttc actgtcgcca
     5581 tcgccgttgt ttcggctgcg gcaggttttt caccattcat catcgccaga atttttttcg
     5641 cgctcggttc gattttatcc gacagcaacg acgggaaaaa tccgaacacg agcaggctga
     5701 tgagcagcgc aagatatgga actttccgca atggccatgt tgattccgcc acggccatca
     5761 atggctccgg caatggccca tgcagcacgt tgcgaatgcc ccgcatcatg taaaccgccc
     5821 cgatcaccag cgcgccccaa acggccaacc cgatgatgaa atggaacgcg ggaactttcc
     5881 acgcgccgaa aaacaccgtc acttccccgg caaaattcgc aaaacccggc agcccgcaac
     5941 cggcgaacat cgccatgatc aacaccgcgc ccacaaagcg catccgccgc agcagaccgc
     6001 ccagcttggc catttccatt gtgccggtct gatgataaat atatccgctc agcccgaacg
     6061 tcagcgccgc caaaaatcca tgcgccacca tcaccagcac cgcgcccgtg atgcccagca
     6121 ggttcaaact cgcgatgccc aaaaacacaa atcccatgtg cgccacactg gaatagccga
     6181 tgagccagtt gaaatttttc tgccgcatcg ccaccagtcc gcaataaacg atgttgccga
     6241 ggcaaagcca cgcgatgatg tgcgcccaat gcgccgcggc ttgcggcaga agcggcacag
     6301 cgagacggat caatccatac aaaccgaatt ttttcaggac acccgcgtgc aacatcgcgg
     6361 tgggcgtcgg cgcggaaccg tacccgagcg gcgcccaggt gtgaaacggc cagagtgaaa
     6421 tcaggatgcc gaaaccaaac aatagcagcg ggaaaataaa attctggtcg tgcagcgaca
     6481 gcggattctg ctgtatatgc tcggtgagtt tcacgatatc aaacgtgtgc gcgccggcct
     6541 ggatatagag cacgatcaat ccgatcaacg cgaccaacgc gccgatgctc aaatacatcg
     6601 tgatctggaa tgtcgcgtaa ttcttgtttt tacctcgtcc ccagacgccg atcatgatga
     6661 acgtcggcac cagcgcgagt tcgtggaaaa aataaaagaa aaacaggtcc agcgacgcga
     6721 acgcgccgag gattccacca gtcatcacca gcagcaggat gtaaaattcc ttttcgcgat
     6781 cgcggatttc ccacgcgcaa cacgccgccg cgaacgccac gattgcgccc atcaagatca
     6841 gcccgacatt caatccatcc acgccgacgt gataatgaa
//
LOCUS       DASZMM010000294         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_685762, whole genome shotgun sequence.
ACCESSION   DASZMM010000294 DASZMM010000000
VERSION     DASZMM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685762"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /gene="gcvP"
                     /locus_tag="VG754_07065"
     CDS             <1..1728
                     /gene="gcvP"
                     /locus_tag="VG754_07065"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HWF19011.1"
                     /translation="KTNAFFDTLRVDLGKKSSAEIIKLAESQRINLRTIDANTIGISL
                     DEAATERDLADLFRIFNGGRVPSFTASELVAQVDADYAAPLARKSPFLTNVVFNTHHS
                     ETEMLRYLRRLESRDLSLTTSMIPLGSCTMKLNATVEMFPVSWPEFSQLHPFAPLRQT
                     QGYQILFQQLEDWLAEITGFAGISLQPNAGSAGEYAGLLVIREYHQDRGQGHRNICLI
                     PQSAHGTNPASAVMAGMQVVAVACDNEGNINVADLKAKAEAHRENLSALMVTYPSTHG
                     VFEETIKEICEIIHANGGQVYMDGANMNAQVGLCRPADMGADVCHLNLHKTFCIPHGG
                     GGPGMGPIGVAEHLVPFLPGHSVVNLGGESPIGAVAEAPWGSASILPISWVYIAAMGP
                     DGLTEATKMAILNANYIAKRLESYFPVLYKGHGDLVAHECILDLRQFKSVTVEDVAKR
                     LMDYGFHAPTISWPVPGTMMVEPTESESKAELDRFCDAMIGIHAEIKAIESGQADKQN
                     NLLKNAPHTADTLVADKWDHPYSREKAGFPAKWLHDHKFWPAVGRIDNVYGDRNLVCS
                     CAGMEAYQS"
     gene            complement(1780..2562)
                     /locus_tag="VG754_07070"
     CDS             complement(1780..2562)
                     /locus_tag="VG754_07070"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HWF19012.1"
                     /translation="MQRLGKNLGKYFGEGIDIQRVLAEIEKSAETHGWKSEAFLKTDH
                     FRLLGLIRSTPRAKKSVYISTGIHGDEPAGPLAALQLLQENRWPDNVDVWLCPCLNPT
                     GFPSSTRENAQGIDLNRQYLQPEAAEIRAHIAWLERQPNFDVCICLHEDWEAAGFYVY
                     ELNPDNRPSFAKAIIARVAEVCPIDMSSMIEGRPASGGIINPSVDPRSRPQWPEAFYL
                     LTHKTRQSYTVEAPSDFQLVTRVAATVAAVRAVLDLLSAQDA"
     gene            complement(2683..>3085)
                     /locus_tag="VG754_07075"
     CDS             complement(2683..>3085)
                     /locus_tag="VG754_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWF19013.1"
                     /translation="LAFALKEVGANVVPINILNPIPGTPFEKVQPLPPLEILKTIACF
                     RFILPRQEIMIAGGRTVNLRDMQSMVFAAGASALMVGNYLTTLNQPVEKDLQMLKDLG
                     LDPSWDKHDFSDQQDVGGGCGSEQCQAEPVV"
BASE COUNT          686 a          865 c          863 g          671 t
ORIGIN      
        1 aagacgaatg cttttttcga cacgttgcgc gttgatttgg gcaaaaagag ttccgctgaa
       61 atcatcaagc tggccgagtc gcaacggatc aatttgcgga caattgatgc gaacacaatc
      121 ggcatctcgc tcgatgaagc ggcgacggaa cgcgatttgg ccgatttgtt ccgcatcttc
      181 aacggaggtc gcgtgccgtc attcaccgct tcggaattgg tcgcgcaggt ggatgccgat
      241 tatgccgcgc cgctcgcccg caagagccca tttttgacca acgtggtgtt caacacgcac
      301 cacagcgaaa ccgaaatgct ccgctatctg cgcaggctgg aatcgcggga cctttcgctg
      361 acgacctcca tgattccact tggctcctgc acgatgaagt tgaatgcaac cgtcgagatg
      421 ttcccggtgt cgtggccgga attttcgcaa ttgcatccgt tcgcgccgtt gcggcaaacg
      481 cagggttatc aaatactctt tcaacagttg gaagattggc tcgcggagat caccgggttt
      541 gcaggaattt cgttacaacc aaatgcgggt tctgctggcg aatatgccgg gttgctggtc
      601 atccgcgaat atcatcagga ccgcggacag ggccatcgca atatctgcct catcccgcaa
      661 tccgcgcacg gcacaaatcc cgccagcgcc gtcatggccg gaatgcaagt cgtggccgtt
      721 gcgtgtgata acgaaggaaa catcaacgtg gccgacttga aggcgaaggc cgaagcgcat
      781 cgtgaaaatc tttctgcgtt gatggtcacc tatccttcca cgcacggcgt gttcgaagaa
      841 accatcaagg aaatctgcga aatcatccac gcgaacggcg ggcaggttta catggatggc
      901 gcaaacatga acgcgcaagt cggcctttgc cgtccggcgg acatgggcgc ggatgtctgc
      961 catttaaatt tgcacaaaac cttttgcatc ccgcacggcg gcggcggtcc cggcatgggg
     1021 ccgattggcg tcgcggaaca tctggtgcca tttttgcccg gccattccgt ggtgaacctg
     1081 ggcggtgaaa gcccgatcgg tgcggttgcc gaagcgcctt ggggcagcgc gagcatcctt
     1141 ccgatttcat gggtctacat cgcggcgatg ggcccggacg gccttaccga ggcgacgaag
     1201 atggcgattc tcaacgcgaa ttacatcgcc aagcggctcg aatcctattt cccggttctc
     1261 tacaaaggcc acggcgacct tgtcgcgcac gagtgcatcc ttgatttgcg ccagttcaag
     1321 agcgtgacgg tggaagacgt cgcgaagcgt ttgatggatt acggatttca cgcgccgacg
     1381 atttcatggc ccgtgcccgg cacgatgatg gtcgaaccga ccgagagcga atcgaaggct
     1441 gagttggatc gtttctgcga cgcgatgatc ggcatccatg ctgaaatcaa agccatcgaa
     1501 tccggccagg cagacaagca gaataacttg ctcaaaaacg cgccgcacac cgcggacacg
     1561 ctggtcgccg acaaatggga ccatccttat tcgcgcgaga aagccggctt tccggccaag
     1621 tggctgcacg accataaatt ttggcccgcc gtcggccgca ttgataacgt ctatggcgac
     1681 cgcaatctgg tttgctcctg cgcggggatg gaagcttatc agagttagtc ggtaagccgc
     1741 tgtccgtgag ctaaattctt ccagccatcc tgaacttctt caggcatctt gtgcgctgag
     1801 cagatccagc accgcgcgca ctgcggcgac cgttgccgcg acgcgtgtga caagctgaaa
     1861 atccgaaggc gcctcgacag tataagactg ccgcgtcttg tgcgtcagca aataaaatgc
     1921 ctcgggccat tgcggacgcg agcgcgggtc cacactggga ttgataatgc cgccggacgc
     1981 aggccggcct tcaatcattg aggacatgtc aatgggacaa acttcggcga cgcgcgcgat
     2041 gatggccttg gcgaatgaag gccgattgtc tggattcaat tcataaacgt aaaaaccggc
     2101 tgcttcccaa tcttcatgca ggcaaatgca aacgtcgaaa ttcggctggc gctcaagcca
     2161 tgcgatatgg gcgcggattt cagcggcttc gggttgcaga tattggcggt tcaaatcgat
     2221 gccttgcgca ttctcgcggg tgctggatgg aaagccagtc ggattgaggc atggacaaag
     2281 ccagacatcc acgttatcgg gccagcgatt ttcctggagc aattgcagcg ccgccagcgg
     2341 cccagccggt tcatcgccgt ggatgccggt ggagatgtaa acactctttt tcgcgcgcgg
     2401 cgtgctgcgt atcaggccaa gaagacgaaa atgatccgtc ttcaagaaag cctccgattt
     2461 ccatccgtgc gtctcggccg acttctcgat ttcggcaagg acacgctgga tgtctatgcc
     2521 ttcaccgaaa tattttccaa gattcttgcc gagccgctgc atcacaccag attgcctcaa
     2581 aatgcagttt gcgccaagcg tgtaaagttt tttagggaag aatcgttgaa aattctggaa
     2641 caactctctg ccgccattgg aatgggattg gctattgcat tgttaaacga cgggctctgc
     2701 ctgacactgc tcactcccac acccgccacc aacgtcttgc tggtccgaaa aatcatgttt
     2761 gtcccagctt ggatccaagc caaggtcttt gagcatttgc aaatcttttt cgaccggctg
     2821 gttcagcgtg gtcaggtaat ttccaaccat caacgcgctt gcgccagcgg cgaagaccat
     2881 gctctgcatg tcacgcagat tcacggtgcg cccgccggca atcatgattt cctgccgcgg
     2941 cagaatgaac cggaaacacg caatcgtctt caagatttcc agcggcggca acggctgcac
     3001 tttttcaaac ggcgttccgg gaatcgggtt gagaatgttg atcggcacca cgttcgcgcc
     3061 cacttccttc aatgcgaacg caagg
//
LOCUS       DASZMM010000295         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3137342, whole genome shotgun sequence.
ACCESSION   DASZMM010000295 DASZMM010000000
VERSION     DASZMM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137342"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VG754_07080"
     CDS             <1..964
                     /locus_tag="VG754_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19014.1"
                     /translation="IEARCYYCQLPVLQGYKHLPDGRYICARELNTVVQSEAEAKEIC
                     RRVESDVDRLLSRFLTIPAENIQLSMQDRYHMEALFNAPGNENSCVTTFGATGTHPLP
                     GHKFVHTVDLLSYMTKTHLMAVCAHEYTHCWMNETISQERKVSLDRNTLEGFCELVAY
                     KYMESLHETAEMEYMKRNNYTHGKILVLIEADKKYGFGTVMDWLVDGEDTTVDSANLD
                     RIRFMRNQRVVSHTPTTTGLIYGTASVPTPVPDTLVLKGISGAGQHRFALINDATFET
                     MEKGKVRVGKTALTMTCLEIGKDFVTIQVDGAKEKQHLSLRPAK"
     gene            1179..1739
                     /locus_tag="VG754_07085"
     CDS             1179..1739
                     /locus_tag="VG754_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HWF19015.1"
                     /translation="MAENSKNLKGQLLLDSGQLSGSFFQRTVVLICQHDSEGAFGLVL
                     NRSSGNKLGEMIVADVPELLKEQTLFLGGPVQLSALSFLHSDDFLPEASVMPNLDLGH
                     SLDTLVELGESFSPTRKMKMFAGYAGWSPGQLEGEMKRKAWIIHPASLDLVFDTEPGK
                     LWQTIMRQKGIKYRLIAEMPEDVSLN"
     gene            complement(1751..>3271)
                     /locus_tag="VG754_07090"
     CDS             complement(1751..>3271)
                     /locus_tag="VG754_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWF19016.1"
                     /translation="LYNRNDIEFTRGQFRVRGDTVELCPAGREDALRIEFFGEEIERI
                     TRFDPLTGHKIEGLAAVTVFPGKQFVTPAQKMKPAILSIREELGERIAWFEKQGKLIE
                     AQRIKMRTEYDLEMMEEMGFCSGIENYTRHISGRAPGSRPTTLFDFFPRDYLLVVDES
                     HATIPQVGGMYAGDHSRKTVLVEHGFRLPSALDNRPLNFDEFQSLQNQTIYLSATPGP
                     REVEWSRGRVVEQIARPTGLVDPRITLKPLKGQIDDLIEESRKRVELKERVLVTTLTK
                     RTAEELSDYLRGIGISVRYLHSEIDAIERVEILRALRKGEFDVLVGINLLREGLDLPE
                     VSLVAILDADKEGYLRSTTSLIQTAGRAARHLNGEVILYADVKTESIQKFLAMSEYRR
                     QRQLAYNKEHNITPRSVSRAVEESLSSHYAINEQANSMLHEKGVDFDVTETLRELEEE
                     MLAAANNLEFEKAALLRDQIRELKRATGDASAKPSDKPRRISYGKGGRGRRAGATR"
BASE COUNT          721 a          951 c          857 g          742 t
ORIGIN      
        1 catcgaagcg cggtgttatt attgccaatt gccggtgttg cagggctaca agcacttgcc
       61 cgacggaagg tacatctgcg cgcgcgagtt gaacaccgtg gttcagtcgg aagccgaagc
      121 aaaggagatt tgccgcagag tcgagtccga tgtggacagg ctgctgtccc ggtttttgac
      181 gattccagcc gaaaatatcc agctctccat gcaggacaga tatcacatgg aagctctttt
      241 taacgcgccg gggaacgaga attcttgtgt cacaacattc ggtgcgaccg gcacacatcc
      301 attgcccggc cacaagttcg tgcataccgt ggacttgctg agttacatga caaaaaccca
      361 tttgatggcc gtgtgcgccc atgaatacac ccactgctgg atgaatgaaa cgatttcgca
      421 agagcggaag gtctcgcttg acagaaatac tcttgagggt ttttgcgaac tcgtcgccta
      481 caaatacatg gaaagccttc atgagaccgc tgagatggaa tacatgaagc gcaacaatta
      541 cacccacggc aaaattctcg tgctgattga agcggacaag aagtatggtt ttggcaccgt
      601 gatggactgg ctggtggacg gcgaagacac aacagttgat tcggcaaatt tggatcgcat
      661 ccgcttcatg cgcaatcaac gcgtcgtcag ccataccccc accacgaccg gcctgattta
      721 tggcaccgcc tcggtcccaa caccggtgcc ggacacgctc gttctgaaag gcatttccgg
      781 cgccggccag catcggtttg cgctgatcaa cgacgccaca tttgaaacga tggaaaaagg
      841 aaaggtgcgc gtgggcaaaa cagcgctcac gatgacctgc ctggaaatcg gcaaagattt
      901 tgtgaccatc caggtggacg gtgcaaagga aaaacaacac ctttctttgc gtccggccaa
      961 atagggacgg gacaaattcc gcaactgtta gttgtggttc atcggcaccc agcctttgcc
     1021 gggaatgtat tcgcgactgc cggggtccgg cgagctgcaa ccggcgataa ccaagctcaa
     1081 cgcgagcaac agaagcatgc aggttcttgt cacgcatgga gtttattcca gattgccaaa
     1141 agggcaagcg tgatgcacat tggcttcggg taagtggcat ggcagagaat tcaaaaaatc
     1201 taaaaggcca gttactgttg gacagcggcc aactgagcgg gtcgtttttc cagcgaacgg
     1261 ttgttctcat ctgccagcac gattcggagg gtgctttcgg cttggtgctc aatcgttcca
     1321 gcggcaacaa actcggcgaa atgatcgtcg ccgacgtgcc cgaattgctg aaggaacaaa
     1381 cgctttttct cggcggcccg gtgcagcttt ccgccctgag ttttcttcat tccgatgatt
     1441 ttcttcccga ggccagcgtg atgccgaacc ttgatcttgg gcattcgctc gacactttgg
     1501 tcgagcttgg cgagtccttt tcgcccacgc gcaaaatgaa gatgttcgcc ggctatgcgg
     1561 gctggagtcc cggccagctc gaaggcgaaa tgaaacgaaa ggcatggatc atccatcccg
     1621 cttcgctcga tttggtgttc gacaccgagc ctggcaaact ctggcaaacc atcatgcgcc
     1681 aaaaggggat caaataccgt ttgatcgcgg agatgccgga ggatgtttcg ctgaattagt
     1741 ttcataatag ttaccgtgtc gcccctgctc ttcttccgcg tccgcctttt ccgtagctga
     1801 ttcgacgcgg tttgtccgaa ggcttggcgc tcgcgtcgcc ggtggcccgt ttcaattcgc
     1861 ggatttggtc gcgcaataaa gcggcctttt caaattctaa attgttcgcc gccgccagca
     1921 tttcctcctc cagttcgcgc aaggtttccg tgacatcgaa atccacccct ttctcgtgca
     1981 acatcgagtt cgcctgctcg ttgatcgcgt aatgcgacga aaggctttcc tccactgccc
     2041 gactcacgct gcgcggggtg atattgtgtt ccttgttgta ggccagttgc cgctggcggc
     2101 gatattcgga catggcgaga aatttctgga tgctctcggt cttcacgtcc gcatataaaa
     2161 tcacttcgcc attcaaatga cgcgcggcgc ggcctgcggt ctggatcaag ctcgtcgtgg
     2221 aacgcaggta accctccttg tccgcatcca aaatcgccac cagcgacact tccggcaaat
     2281 ccagcccttc gcgcaaaaga ttgatgccca ccagcacgtc gaattccccc ttgcgcaacg
     2341 cgcgcaaaat ttccacccgc tcgattgcgt caatctcact gtgcagataa cgcacactga
     2401 tgccgatgcc acgcagataa tcgctcaatt cctcagccgt gcgtttggtc aacgtggtga
     2461 ccaacacccg ctccttcagt tcaacccgtt tgcgcgactc ctcgatcaga tcgtcaatct
     2521 gccctttcaa cggcttgagg gtgatgcgcg gatccaccaa cccggtcggg cgcgcaatct
     2581 gttccacgac ccgcccgcgc gaccattcga cttccctcgg acccggcgtg gcgctgaggt
     2641 aaatcgtctg gttttgcagc gattgaaact cgtcgaaatt cagcggccga ttatcaagcg
     2701 cgctgggcag ccgaaaacca tgctcaacca gcaccgtctt gcgcgagtgg tctcccgcat
     2761 acatcccgcc gacctgcgga atcgtcgcgt gcgattcgtc caccaccagc agataatccc
     2821 ttgggaagaa atcgaacaac gtcgttggcc gcgaaccggg cgcgcgtccg ctgatgtgcc
     2881 gcgtgtaatt ctcgattccc gaacaaaagc ccatctcctc catcatctca aggtcgtatt
     2941 ccgtccgcat cttgatgcgt tgcgcctcga tcaatttgcc ctgcttctcg aaccaagcga
     3001 tgcgttcgcc aagctcttcg cgaattgaca agattgccgg tttcattttc tgcgcaggcg
     3061 tcacgaattg tttgccggga aaaaccgtca ccgccgcaag cccttcaatt ttgtgcccgg
     3121 tcaacgggtc aaagcgcgtg atgcgctcaa tctcctcgcc gaaaaattcg atgcgcaacg
     3181 cgtcttcacg tcccgcggga caaagctcga ccgtgtcgcc gcgcacgcgg aattgcccgc
     3241 gcgtgaattc gatgtcattg cggttgtata g
//
LOCUS       DASZMM010000296         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5055598, whole genome shotgun sequence.
ACCESSION   DASZMM010000296 DASZMM010000000
VERSION     DASZMM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5055598"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2833
                     /locus_tag="VG754_07095"
     CDS             <1..2833
                     /locus_tag="VG754_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF19017.1"
                     /translation="MNPDTGTFEPVAGLTQQGRGRDDWGNWFGCDNSNPLWDYPLPDH
                     YVRRNPYITAPSPRVFAATGPEPNLVHPISRMLERFNEPNAANHVTSGCGLGIYRDTL
                     LGEDFYGNTFTCEPVGNLVYRLQLKEDEDGAVVMGTRPDDEKDSDFLASRDNWFRPVE
                     ARTGPDGALYVVDMYRFVIEHPRWIGKERLAQLDVRAGADKGRIYRIYPADKKLRPVP
                     NLKKLSTVKLVAALDTPNGTLRDLIHIELVQRNNESAVRPLSELATKSKSPAVRVQAL
                     CVLDGMKALSPDLLVTALRDPDVHVRRQAIRLSETRVASSPALQKAVLKLADDPGVSY
                     QLALSLGEWNDSRAGQALGKLAQSKMDDGWIRAAVLSSAIPHTVEILKGVLAASSATP
                     GRNEMVSQLIATAVGKGDPETLGQIVATVVPKEGEQIEDWQFSVLKNLLDALRRKKQS
                     ISSFVAGAAAKDRDVPFRIERMFDAARKLAGDSTASESSRVIAIRLLGQDTTREEADL
                     QLLGSLLKSSNPLPVQKAAVGTLQYNSSAKVPGLLLADWKQLSPSLRAPILEILMSRE
                     EFTKALLTAIQNRTVQANEVPAASVQRLLKHSNADIQKQAAALLTTGPTRSRAEAMAR
                     YQICTTLKGDAVIGGKIFAKNCTPCHLVKGQGNAVGPDLSLLRDKTPSDFVLAIIDPN
                     AAVEPRFLAYDIETKDDRSLVGVISAESSTSLTFVQPGGIQEKILRSDIAEIKATGLS
                     LMPEGFEQSINQQELADLIAYLKTAPAQFGSADGERAASALAQFRAEGNSLAKVVSAS
                     EEISYASWLGTLPLEHCRQTDGKSRVIWQSAPLPAELKAGGSQQFHFPIGMGWFSAPP
                     GKFTLSINGQPALEFNVALDDHAWQSADGKVQMSYTVMEKNSEDSNGALLIEVPNSLF
                     EPGKPVTFEVTGSASDSQRWFGIYRIAAN"
BASE COUNT          675 a          856 c          863 g          625 t
ORIGIN      
        1 catgaacccg gacaccggca cgttcgaacc ggtcgccggg ctcacgcagc aaggccgcgg
       61 acgcgatgat tggggcaact ggttcggctg cgacaacagc aacccgcttt gggattatcc
      121 gttgccggac cattacgtgc ggcgcaatcc gtacatcacc gcgccgtcgc cgcgcgtttt
      181 tgccgccacc gggcctgagc caaatcttgt ccatcccatc agccgcatgc tcgaacgctt
      241 caacgaaccg aacgcggcca atcacgtgac ttccggttgc ggccttggca tttatcgcga
      301 cactttgctc ggcgaagatt tttacggaaa tacattcacc tgcgaaccgg tcggcaacct
      361 cgtttaccga ttgcaactca aagaagatga agatggcgcg gtcgtgatgg gcacgcggcc
      421 tgacgacgaa aaggattcgg attttctcgc gtcacgcgat aattggtttc gtccggttga
      481 ggcgcgcacc gggccggacg gcgcgttgta tgtcgtcgat atgtatcgtt tcgtcatcga
      541 gcatccgcgc tggattggga aggaacgcct tgcccagctt gatgtccgcg ccggcgccga
      601 taaagggcgc atttaccgca tctatccggc tgataaaaaa cttcggccag ttccgaatct
      661 caaaaagctt tccacagtga aacttgtcgc ggcgcttgac acgccgaatg ggacattgcg
      721 cgatttgatc catattgaac ttgtccagcg caacaatgaa tctgccgtgc gcccgttgtc
      781 cgagcttgcc acaaagagca aatcgcccgc cgtgcgtgtc caggcgctct gcgtcttgga
      841 cggcatgaag gcattgtctc ctgatttgct cgtgaccgcg ttgcgtgatc cggatgttca
      901 tgtgcgccgc caggcgattc gattgagcga aacacgcgtt gcttcatcgc ctgcgcttca
      961 aaaagcggtg ctgaagctcg ctgatgatcc gggtgtgagt tatcaactcg cgttgagtct
     1021 cggtgaatgg aatgattcgc gcgctggcca agctttgggc aaattggcgc agtcaaagat
     1081 ggatgatgga tggattcgcg cagccgttct cagttccgcc attccgcaca cggtcgaaat
     1141 tctcaaaggt gttcttgccg cgagttccgc aacgcccggt cgcaacgaaa tggtgagcca
     1201 attgattgcc accgccgtgg gaaaaggcga tccggaaacg ctcggccaaa tcgtggcgac
     1261 tgtggttccg aaagagggcg agcaaattga agactggcaa ttttcagtgc tgaaaaatct
     1321 gctcgatgcg ttgcgccgca aaaaacaatc catctcgtcc tttgtggctg gcgctgccgc
     1381 gaaggaccgc gatgtccctt ttcgtatcga gaggatgttc gacgccgctc gcaagctggc
     1441 gggcgattca acggcaagcg aatcatcgcg ggtcatcgcc atccgtctgc ttggacagga
     1501 cacgacacgt gaagaagccg acctgcaatt acttggcagt ttgttgaagt cgtcaaaccc
     1561 tttgccggtg cagaaggccg ccgttgggac gcttcaatac aacagcagcg cgaaggttcc
     1621 cggacttttg ctggcggatt ggaagcagct ttcgccatcg ttgcgcgcgc cgatattgga
     1681 aattctcatg agccgcgaag aattcaccaa ggcgttgctg accgcgatcc aaaatcgcac
     1741 ggtgcaagcc aatgaagttc cggccgcaag cgttcaacga ttgttgaaac attcgaacgc
     1801 ggacatccaa aaacaagccg ccgcgctgtt gaccaccggc ccgacgcgca gccgcgcgga
     1861 agcgatggcg cgttatcaaa tctgcacgac gttgaaaggc gacgcggtca tcggcgggaa
     1921 aatttttgcg aagaattgca cgccctgtca tctggtcaaa ggccagggca atgcggtcgg
     1981 cccggacctt tccttgttgc gcgacaaaac gccaagcgat ttcgtgctgg ccatcattga
     2041 cccgaatgcc gcggtcgagc cgcgttttct cgcgtatgac atcgagacga aggatgatcg
     2101 ttcgctggtg ggcgtcatca gcgcggaaag ttccacatcg ctgacgtttg tgcaaccggg
     2161 cggcatccag gaaaaaatcc tgcgcagcga catcgcggaa atcaaggcaa cgggactttc
     2221 gctcatgccc gaaggttttg agcaaagcat caaccagcag gaacttgccg atctcatcgc
     2281 ttacttgaag accgcgccgg cgcagtttgg cagcgcggac ggcgaaaggg ccgcaagcgc
     2341 cctcgcccaa tttcgggccg aaggcaattc acttgcgaag gtggtgtccg cttcggaaga
     2401 aatttcttac gcaagctggc ttggcacact gccgcttgag cattgccggc agaccgacgg
     2461 caaatcacgg gtgatttggc aatccgcgcc gttgcccgcc gaactcaaag cgggcggttc
     2521 gcaacaattc cattttccca tcggcatggg ctggttctca gcgccccccg gcaaattcac
     2581 cttgagcatc aatggccagc ctgcgctcga attcaacgtg gcgctcgacg accacgcctg
     2641 gcaaagcgcg gacggcaaag tgcagatgag ctataccgtg atggaaaaaa attctgagga
     2701 cagcaacggc gcgttgctca tcgaagttcc caactcgctt tttgaaccgg gcaagcctgt
     2761 cacgttcgaa gtcaccggat cagccagcga cagccagaga tggttcggca tctatcgcat
     2821 cgcggcgaat tgatggaaat gttttgcgcg aaaaagtgag tcaagccaga accaaaccat
     2881 ggttggtaaa cggctgaaaa tccgctgtgt tgatcgttgc gagctttgcg ccacaacgaa
     2941 tggcaactgc cgcaatcatg caatcggcca aacttctgga acgccgccca gtctggttga
     3001 acaatttcgc ggcgagatc
//
LOCUS       DASZMM010000297         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4252227, whole genome shotgun sequence.
ACCESSION   DASZMM010000297 DASZMM010000000
VERSION     DASZMM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252227"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VG754_07100"
     CDS             complement(<1..1502)
                     /locus_tag="VG754_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWF19018.1"
                     /translation="MMKRLGNVGRTCGLGAFLLFQLIALLSFAQDGSNVPLSSVQNRH
                     DGNPHKIQVTDPLLARKIEAQGGHLIADYGSYQLYEIPQLVPGMFANTRAELRDGYDV
                     IELNAGHLNTRRPEVQALTKAVGTFSGKRLHLVQFVGPVQTQWRNELLAAGVEIISYI
                     PENAYLIYGDSKSLAAVQNLAAKKSNYQWQGAFRDEYKIHPKARNVDERGNPRNIGTD
                     LFDIQMVYDPKANQGTLNLLNQLKLEPFERLETNFHRIALVVRLNPDDISKIAVQPEI
                     ISILPYYHRQKLCERQDQIVAGHLTNGVPTGPGYLCWLTNLGFTQAQFTASGFAVDVS
                     DSGVDNGTTSPNHFGLYTGGSVSNASRIVYARYESTVTYDTNSLEGCDGHGNLNAHII
                     GGYDDFSGFPFTDSSGFHYGLGVCPFVNIGSSVIFDSAQSDHFYFPNFNNLQAEAYRD
                     GARVSCNSWGATNSQGVYDSEAQKYDALVRDAQLANSTVPMDGNQGMIIV"
     gene            complement(1726..>4110)
                     /locus_tag="VG754_07105"
     CDS             complement(1726..>4110)
                     /locus_tag="VG754_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF19019.1"
                     /translation="VESAGTKYGLILLGITVAPSNPEKLTDTIAAAQSPFPDLRLRQG
                     EPAQAQGIVKTRDGKAIARAIVRVSAIREFNTDDKTAVQTGPGVGLGTETDENGRFIL
                     PPLPDNKLYRLVVWSPGYESYRYRGMDAKSDPIEIHLKPQKDSSPNPKFAIHGRVIGP
                     DRKPIPFALVEHDGIGYDGGTSWGGNAEGLPGDVISDAKGEFIFGRDKGFNRLQISIK
                     IPDLAPVHRWFEVTNSVQDVTMGVGATLRGRVVKDGQPLTNLAVQACGSDRNSEVFAG
                     VFDAKTGTNGEFLFEHLPPNISWDVYGKLSSFKEYGALPPRIVISAGDGESTDLGDLQ
                     VVPAIHLAGQVKTRNGEPLPKGLKVSAGYNEEWDGESAAVDDKGHFLLEGLSPRQVDI
                     SIQERNWLLTVANRSLDTWNPWSLTGLLEGNKDDLLLVIDKGEPNYNGNYSSANGQLP
                     PADQSRSRPLWGAEDSGPPYIVLAGSVVDDTTGQPIKNFKIIPGHKPPVSAMPMAQAP
                     KSLLKKVLDPFAGSKPVVPWNERIYWQYSSTETMSNGAFSVPFIPLTSTPVLRVEASG
                     YQPLETDPTNQTTTNLVLRLKKGAGPNGVVLLPNGSLATRATVVYAVNQEQFSLEGRS
                     LSNYGQRGSQKVTGVDGKFSFERRAEESMIFVSHPTGWGQQSLDRGRENLTIELKPWA
                     SISGTLVNSNNAPMPGVKLAVTMFHNWNQGGAYLNLQEQTVTDAQGHFLFTNVPPARV
                     EVQRVIPFAGGGWSYQEQSWLFVQPGITNDLGKVTYDHPPPAPAMERFKQQLGL"
BASE COUNT          930 a         1063 c         1103 g         1014 t
ORIGIN      
        1 aaaacgatga tcatcccctg gtttccatcc atgggaacag tggagttcgc gagttgcgcg
       61 tcgcggacga gagcgtcgta tttctgcgct tcagaatcat aaactccttg cgagttcgtt
      121 gctccccaac tgttgcaact cacgcgcgcg ccatcgcgat aggcttccgc ctgaagattg
      181 ttaaaattgg gaaagtaaaa atggtcggat tgcgccgaat cgaatatgac cgatgaaccg
      241 atgttcacga acggacagac gccgaggcca tagtgaaacc ctgaactgtc cgtgaatgga
      301 aaaccggaga agtcatcata accgccgatg atgtgcgcgt tcaggttgcc atggccatcg
      361 cagccttcga gagaatttgt atcataggtg acggtgcttt cgtaacgcgc gtaaacgatg
      421 cggctggcgt tactcacgct cccgccggtg taaagaccaa agtgattcgg agaagtggtg
      481 ccgttgtcaa caccgctgtc cgagacatcc acggcaaagc ccgacgcggt gaattgcgcc
      541 tgcgtaaatc ccaaattggt caaccaacat aagtatcccg gtcctgtcgg aacgccattg
      601 gtcaaatgtc ccgcgacgat ctggtcctgt cgctcgcaca atttttggcg atgatagtat
      661 ggcaggatgg aaatgatttc aggctgcacc gcgattttcg aaatgtcatc gggattcaat
      721 cgaacgacca aagcgatacg atgaaaattg gtctccaatc gctcgaacgg ctccagtttg
      781 agttgattga gcaaatttaa ggtgccttgg ttcgcttttg ggtcatacac catctggatg
      841 tcgaacaaat cagtgccgat gtttcgcgga ttgcctcgtt catccacatt gcgcgccttg
      901 ggatgaattt tgtattcgtc ccggaatgcg ccctgccatt ggtaattcga ttttttggca
      961 gcgagatttt gcacggcggc aagacttttt gaatcgccgt aaatcaagta ggcattctcc
     1021 ggaatgtagg aaatgatttc cactccggct gccagtaatt cattccgcca ttgtgtctgg
     1081 acaggaccaa cgaattgaac cagatgcagc cgcttgcctg aaaaggttcc gactgctttg
     1141 gtgagcgcct gcacttccgg cctgcgcgtg ttgaggtgcc cggcgttcaa ttcgatgacg
     1201 tcataaccgt cgcgtaattc ggcgcgggtg ttcgcaaaca ttccggggac aagctgcgga
     1261 atttcgtaga gctgatacga gccatagtcc gcaatcaagt ggccgccttg cgcttcgatc
     1321 tttcgcgcaa gcaatggatc ggtgacctgg attttgtgag gattgccatc atggcgattt
     1381 tggacagagg ataaaggaac atttgaaccg tcttgcgcaa aggaaagcag cgcaatcagt
     1441 tggaacaaca ggaaagcccc caaaccacag gttcgtccca cgttgccgag gcgtttcatc
     1501 atttatcaag tagctgttaa tgtgccatgc tgtcccattc ttactggtga gtacttgacg
     1561 gacagcggct tgcgagaaaa tccttggctt ataggcacaa ctatagcagc acgtttttcg
     1621 gctttccaac tcatgaaaga cccataatgc gtgaaaattc agcgccgggc aaggcgcaaa
     1681 tgttccgaaa cgccaataaa ccgactgttt attattatct aaagcttata atccgagttg
     1741 ttgcttaaac cgttccatgg ccggagcggg cggaggatga tcataagtca ctttgccgag
     1801 gtcattggtg atgcccggct ggacaaagag ccacgattgt tcctggtaac tccagccacc
     1861 gccagcaaag ggaatcactc gctgcacttc aacgcgcgcg ggcggcacat tggtgaagag
     1921 aaaatgtccc tgcgcatcgg tgaccgtctg ctcttgcaga ttgaggtatg cgccaccttg
     1981 attccaattg tgaaacatcg ttacggccag ttttacgcct ggcattggag cgttgttgct
     2041 gttgacgagt gtgccggaga ttgaagccca tggcttgagt tcaatggtca gattttccct
     2101 gccgcgatcc agagattgct ggccccaccc tgtgggatgt gaaacaaaaa tcatgctttc
     2161 ttctgcgcga cgttcaaagg aaaatttgcc atccacgccg gtgactttct gcgaacccct
     2221 ttggccataa tttgaaagag acctcccttc gagtgaaaat tgttcctggt tgacggcata
     2281 tacgactgtg gcgcgcgtcg cgagacttcc gttgggcaat agaacaacgc cgtttggtcc
     2341 ggcgcctttt ttaaggcgta aaacaaggtt cgtcgttgtt tgatttgtgg gatcggtttc
     2401 cagcggttga taaccggatg cctcaacccg cagcaccggc gtgctggtca ggggaatgaa
     2461 gggaactgag aaagcgccgt tcgacatggt ttcggtgctg gaatattgcc agtaaatcct
     2521 ttcgttccag ggaacgaccg gtttgctgcc cgcaaaggga tcgaggactt ttttgagcaa
     2581 tgatttcgga gcctgcgcca tgggcatggc gctgaccggc ggtttatggc cgggaatgat
     2641 tttgaaattc tttatcggct ggccggttgt gtcatctaca acgctgcccg cgagaacgat
     2701 gtaaggcggc ccggaatctt cagcgcccca caatggccgt gagcgcgatt ggtccgcggg
     2761 cggcaactgg ccgttcgcgc tgctgtaatt cccattgtaa ttcggttcgc ctttatcaat
     2821 gacaaggagc aaatcgtcct tgttgccttc cagcaggcct gtcaaagacc atggattcca
     2881 tgtgtccaaa ctgcggtttg caactgtgag cagccaattc ctttcttgaa tggaaatgtc
     2941 cacctgtcgg ggtgaaagtc cctcaagcag aaaatggccc ttgtcgtcaa ctgccgccga
     3001 ttctccgtcc cattcttcat tataaccggc gctgaccttg agtcctttcg gcagcggttc
     3061 gccattgcga gttttgactt gtccggccag atggattgcg ggcacgacct gcaagtcgcc
     3121 gaggtcggtg ctttcgccat caccggcgga aatcacaatg cgcggcggca acgctccgta
     3181 ttccttgaag gaagagagtt tgccgtaaac atcccacgaa atatttggcg gaagatgttc
     3241 gaagaggaat tctccgttcg ttcccgtttt ggcatcgaat actcccgcaa aaacttccga
     3301 attgcggtcc gatccacaag cttgaacggc aaggttcgtc aagggttgtc cgtccttcac
     3361 cacgcgcccg cgcaaggtcg cgcccacgcc catggtcaca tcttgcacac tgtttgtgac
     3421 ttcaaaccaa cggtgcaccg gagcaaggtc gggaatcttg atgctgatct gcagccggtt
     3481 aaaacccttg tcgcggccaa agatgaactc gcctttggca tcgctgatca catcgccggg
     3541 gagaccttca gcgtttcctc cccagcttgt gccgccatcg taaccaatac cgtcatgctc
     3601 gaccaaggca aacggaatcg gttttcggtc aggaccaatc acacgtccat ggattgcgaa
     3661 ttttggattc ggtgaactgt ccttctgcgg tttcagatgg atttcaattg ggtcggactt
     3721 ggcatccatg ccgcgatagc gataggattc gtaaccgggc gaccatacga cgaggcggta
     3781 gagcttgttg tccggcaacg gtggcaaaat aaaacgaccg ttctcgtcag tttccgtgcc
     3841 caaacctaca ccgggacctg tttgcacggc ggttttgtca tcggtattaa attcacggat
     3901 cgcggagacc cggacaatcg cgcgcgcgat tgctttgccg tccctggttt tgacgatgcc
     3961 ttgggcttgc gccggttcgc cttggcgcag acgcaaatct ggaaatggac tctgcgccgc
     4021 agcaattgta tcggttaatt tttccggatt tgagggcgca acggtgatac ccaataaaat
     4081 taaaccatat tttgtgcctg cggactcgac
//
LOCUS       DASZMM010000298         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4252981, whole genome shotgun sequence.
ACCESSION   DASZMM010000298 DASZMM010000000
VERSION     DASZMM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252981"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VG754_07110"
     CDS             complement(<1..1111)
                     /locus_tag="VG754_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19020.1"
                     /translation="MKKFAERKEAQARALNKQQSYRWPGEATKFFKAAEAGDWQEASN
                     RYTKLLREYIAFHAPTAKTNSWDKFMMKTYALGSRVGILPINYWPNLRGPQWSPIDEV
                     NGAYAVFQKWDPDLLLYFATNIIASIPTNSIYFGGSDEARYVVSALCKNQELGEPFFT
                     LTQNRLADPTYRKYLNQMYGKKIHISTDAESRQAFSDYMTEVEKRADQGKLKPGENFS
                     SSGGRVRISGMGAMMEINGVMVRQIFDANTNHEFFIEESWPLDWMYPYLVPHGLILKI
                     NRLPLAKLDESTVAADRAFWEGVVEKFIGLKISNETPLGELEKFAEQVYLQKDLRDFK
                     GDKRFAANEMAQARFGKLRASIAGVYAWRAQQPGDE"
     gene            1151..1357
                     /locus_tag="VG754_07115"
     CDS             1151..1357
                     /locus_tag="VG754_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19021.1"
                     /translation="MPAASYQNCHEKAQKAQAQTEKNQTGLHTAPMTLFRRMKSHVKN
                     RHRQCPICMTSDSFRQTPNQPEKI"
     gene            complement(1462..2625)
                     /locus_tag="VG754_07120"
     CDS             complement(1462..2625)
                     /locus_tag="VG754_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19022.1"
                     /translation="MSKTLRVGMIGHRFMGKAHSNAWRQAPRFFPLKANAEMVTICGR
                     DAAAVEAARVQFGWQHASADWRDVVNSPDIDIIDVTTPNESHAEISIAAAKAGKHVLC
                     EKPLAMSVKQAEEMVAAVKKSGVVNMICHNYRRIPAIAQAKKMIDEGVLGRIYHFRAR
                     YGQDWASSPDVPLIWRMQKGASGSGALGDLNAHIIDLGRYLVGEFKEVCGMMDTFIKE
                     RPLLSSAKGTGIGGGKTGKAMGKVTVDDSAVFIGHFTNGAVANLEATRFAPGRKNHIE
                     IEINGSKGSFFFDFEDMNRLKYYDNTAPLDRQGFADILVTQGNGIHPYFGAWWPIGHI
                     IGYEHTFIHTVVDFVNACVAGKSVQPTFEDGLKNQRVLEAVEKSCNTRGWVKV"
     gene            2746..3645
                     /gene="metF"
                     /locus_tag="VG754_07125"
     CDS             2746..3645
                     /gene="metF"
                     /locus_tag="VG754_07125"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HWF19023.1"
                     /translation="MQFVRDIYAARRAARKPVISFEFFPPKTDEGDRTLLEKTLPALL
                     RLKPDFCSVTYGAGGGTRDKTLMIVDRIQREHNLTTVLHLTCVNATRDEIGAVVNQAR
                     SLGIKNILALRGDPPGGNGVFQKTEGGFEFSYQLVTYLKELGGFSIGTAGFPEGHIAC
                     TEGKYVDWERLKNKVNSGADFVITQLFYDNADYFEFRDHLTKKLGMNVPIFPGILPVL
                     SAGQTRRITSLCGAKLPSALVAGLEKFADNDEAAMQFGIDYATEQCAELLRSGVAGLH
                     MYTLNKAHSTTQVLKNLGLVLGG"
BASE COUNT          822 a         1067 c          975 g          856 t
ORIGIN      
        1 cttcatctcc cggttgttgc gcgcgccatg catacactcc cgcgatggac gcacgcaact
       61 tcccgaaacg cgcctgcgcc atttcgttcg ccgcgaagcg tttgtctccc ttgaaatcgc
      121 gcaagtcctt ttgcaaatag acctgctccg caaatttctc aagttcaccc agcggcgttt
      181 cattgctgat tttcaatccg atgaatttct cgaccactcc ctcccaaaaa gcgcggtcag
      241 ccgccaccgt gctttcatcc aatttcgcca atggcagccg attgattttc aaaatcagcc
      301 catgcggaac cagataggga tacatccaat ccaacggcca gctttcttcg atgaaaaact
      361 cgtgattggt gttggcgtcg aaaatttgcc gcaccatgac gccgttgatc tccatcattg
      421 cccccatgcc actgattctg acgcgtccgc ccgaggacga aaaattttcc ccgggtttca
      481 atttcccctg gtccgcccgt ttttcaacct cggtcatgta atcagaaaac gcctgccgag
      541 attctgcatc ggtcgaaatg tgaatttttt tcccatacat ctggttcaga tatttccggt
      601 aggtgggatc agcgagcctg ttttgagtga gtgtgaaaaa tggctcgcct agctcctggt
      661 ttttgcagag cgcggacacc acgtaacgcg cttcatcgct gccgccaaaa taaatgctgt
      721 ttgtggggat ggaggcaatg atgttcgtgg caaagtacaa caacaaatcc gggtcccatt
      781 tttgaaaaac tgcgtacgcg ccattcacct cgtcaatcgg tgaccattgc ggccccctga
      841 gattcggcca ataatttatc ggcaaaatcc ccacgcgtga tcccaatgcg taagtcttca
      901 tcatgaactt gtcccatgaa ttcgtttttg ccgttggagc gtgaaatgcg atatattcgc
      961 gcaaaagctt ggtgtagcga ttggatgcct cctgccaatc gcccgcctcc gccgccttga
     1021 aaaacttggt cgcttcgccg ggccagcgat acgattgctg tttgttcaac gcccttgcct
     1081 gcgcctcctt acgttccgca aacttcttca acgccttctt caggtcagac gacgatgacg
     1141 cctcatcctt atgcctgcag ccagctacca gaattgccac gaaaaggcac aaaaagcaca
     1201 agcccaaacg gagaaaaacc aaaccggact gcacaccgcg ccgatgacgc tttttcgccg
     1261 tatgaaaagt catgtaaaaa atagacaccg ccaatgcccg atttgtatga ccagcgactc
     1321 cttccgtcaa acaccaaacc agcctgaaaa aatttaaccg cagatggact cagatgaacg
     1381 cggatgaaga aaactgaatg acacgaattt cacgaatttt tcatcttgag tcaaattccg
     1441 tgttcaatcc gtggctgcaa ttcaaacctt gacccagccg cgggtgttgc aggatttttc
     1501 gaccgcttcg agcacgcgct ggtttttcag gccgtcctca aaagtcggtt ggacggattt
     1561 gccggcgacg caggcgttca cgaaatcaac gacggtgtgg atgaacgtgt gttcgtaacc
     1621 gatgatgtgg ccgatgggcc accacgcgcc gaagtaagga tggatgccgt tgccttgcgt
     1681 cacgaggatg tccgcgaaac cctggcggtc caagggcgcg gtgttgtcgt aatatttgag
     1741 gcggttcatg tcctcgaaat cgaagaagaa ggaacctttc gagccgttga tctcgatctc
     1801 gatgtggttc ttgcggcccg gcgcgaaacg cgtcgcttcg agattggcca ccgcgccatt
     1861 cgtgaaatgg ccgatgaaca ccgccgaatc atccaccgtc actttgccca tcgccttgcc
     1921 ggttttgccg ccgccgatgc cggttccttt ggccgaggag agcaaaggac gttccttgat
     1981 gaaggtgtcc atcatgccgc aaacttcctt gaattcgccg acgagataac ggccaaggtc
     2041 aatgatgtgg gcattaaggt caccgagcgc gccgctgccg cttgcgccct tttgcatgcg
     2101 ccagatgagc ggcacatccg gactcgaagc ccaatcctgt ccgtaacgcg cgcgaaaatg
     2161 ataaatgcgt ccgagcacgc cttcatcaat catctttttc gcctgcgcga tggcggggat
     2221 gcggcggtaa ttgtggcaaa tcatgttgac cacgccggat tttttgacgg cggcaaccat
     2281 ctcttcggcc tgcttgacgc tcatggcgag cggtttttcg cagaggacgt gcttgccggc
     2341 tttcgcggcg gcgatggaaa tttcggcgtg cgattcgttc ggcgtcgtga cgtcaatgat
     2401 gtcaatgtcc gggctgttga ccacgtcccg ccagtcagcc gaggcgtgtt gccagccgaa
     2461 ttggacgcgg gcggcttcga ctgcggcggc atcgcgtccg caaatggtga ccatctcggc
     2521 attggccttg agaggaaaaa aacggggggc ctggcgccag gcattggaat gcgcctttcc
     2581 catgaaccga tgcccgatca tcccgacacg caatgttttg ctcatattcg taaattttta
     2641 aagcgcaaca gggtagccat ggggattgtt tctagtcaag tggccgggag aagccgcgca
     2701 acgcgtgctt tccttttcgc cgaaaccgtg cataattgtg aaccaatgca attcgttcgc
     2761 gacatttatg cggcccgccg ggcggcaagg aaaccggtga tttctttcga attctttccg
     2821 cccaagacgg acgaaggcga ccgcacgctg ctcgaaaaga cgctgcctgc gttgttgcgt
     2881 ctcaagcccg atttctgctc ggtgacttat ggcgcaggcg gcggcacgcg cgacaagacg
     2941 ctcatgattg tggaccgcat ccagcgcgaa cataatctca ccaccgtcct gcacctcact
     3001 tgcgtcaacg ccacgcgtga tgaaatcggc gcggtcgtga accaggcgcg ctcgcttggc
     3061 atcaaaaaca ttctcgcctt gcgaggcgat ccgccgggcg gcaacggcgt gttccaaaaa
     3121 accgagggcg gctttgagtt ttcctatcag ctcgtcacct atttgaagga gcttggcggc
     3181 ttttccatcg gcaccgccgg gtttcccgag ggccacatcg cctgcacgga aggcaaatac
     3241 gtggattggg aacggctcaa gaacaaggtc aatagcggcg cggactttgt catcacgcaa
     3301 cttttttatg acaacgcgga ttactttgaa ttccgcgatc acctgacgaa aaagctcggc
     3361 atgaacgtcc cgattttccc gggcatcctg ccggtcctca gcgcgggcca gacgcggcgc
     3421 atcacatcct tgtgtggcgc gaaattgccg tccgcattgg tcgccggctt ggaaaaattc
     3481 gccgacaacg acgaagccgc gatgcaattt ggcattgatt atgccacgga acaatgcgcc
     3541 gaactcttgc gctcaggcgt cgccggcctg cacatgtaca cgctcaacaa ggcccattct
     3601 acaacgcagg tgttgaagaa tctcggattg gtgctgggcg gataatttca atgatctacg
     3661 acgcgacatt ggatgaattg ctgcactcgg tttgggacgg caagcgcatc aacgccgccg
//
LOCUS       DASZMM010000299         6684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_112032, whole genome shotgun sequence.
ACCESSION   DASZMM010000299 DASZMM010000000
VERSION     DASZMM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112032"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="VG754_07130"
     CDS             complement(<1..1575)
                     /locus_tag="VG754_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HWF19024.1"
                     /translation="MTLPTYLPARPSVHPMFLEKRVYQGSSGRVYPLPFTDRIAETPV
                     DRKWKVIWLENEFLRVMILPEIGGRIHAILDKTNGYDLIYNQRVIKPALVGLAGPWVS
                     GGIEFNWPQHHRPATFLPTDSEIEKHADGSATAWCSDHDPMARMKGMHGVCLHPGKAY
                     VELKVRAYNRTEFAQTFLWWANVATRVHEAYQSFFPPDVQYVADHARRSMSAYPLAQG
                     HYYGVDYKQRARDGVPKGEAPRQFVPPHCREKNDSSGNVPDMPCYQPNDLSFYANIPT
                     PCSYMCVGSKEDFFGGYDYKRQAGIVHIADHHISPGKKQWTWGNHEFGYAWDRNLTEP
                     DENGEYAPYIELMAGVFTDNQPDFSFLQPGETRAWSQYWYPIQKIGPAQMANLEAAIS
                     LKVVKQKARIGVCVTAPGNSLKVQLSARGEMVFARDCELTPEKPLVEEIELPEGVLET
                     ELRLRVLDADDREIIGYQPSTPLRGKAPLPATEPPLPKDIATADELLITGLHLDQYRH
                     ATRCPTPYWLEALRRDP"
     gene            complement(1729..2562)
                     /locus_tag="VG754_07135"
     CDS             complement(1729..2562)
                     /locus_tag="VG754_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF19025.1"
                     /translation="MKKSVLFAGNPGALRKGNAPGFTLIELLVVIAIIAILAALLLPA
                     LRAAKVRAIRIKCVSNERQLGIALTMYADDFGEYYPVYQDWGCWAGQKGSGQPQAEYG
                     FQYPDGLRPLNSYLKNVNTCYCPGDKGDPGASGGTMNWTPSQTCFADWGNSYLMPWRQ
                     PGLISASGGQNGTYGWSYYGIESIGGGTNSSGSTPAMKRTEMVEKISTKIILVDWPGA
                     PDRPLDMVSAWHAVNGVGLFNILYADSHVSGYLFTAAERDSSTNSMWGATVDPALRGY
                     W"
     gene            complement(2565..5633)
                     /locus_tag="VG754_07140"
     CDS             complement(2565..5633)
                     /locus_tag="VG754_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF19026.1"
                     /translation="MNTNRTTFLSLRAIMAAGILFAVAVSSQAQVTQMLYATPTNAAR
                     DDYSGVVGTQFQVGSSNVIVSHLGVFDINNDGLAVSHQAGVFTTSLTVLGQVVVPSGT
                     DAYLTNGFRWVPLDPPVLLSSNTSYIVGGVVSNADGDTWQTTFIPTWNTFFVGSTTTD
                     SRTAVYGAGYAPGVPIAWPPSGFTQNSTNDAYGNVTLGYIEIDQARVGVQTTNISIGT
                     GQTLSVLGFASGQPPITYQWYLAPNTLLTSQTNATLTIPNAATNNSGTYFLTASNVLG
                     GEQSSNVTVLITSTPVSISKQPTNLTVFANYPAAFSVTATGTPVIAYQWSRNSTAIPG
                     ATNSSLSFVAASTNNGDVYSCLVSNNISSTNTANATLTVVANFAFPTEYLHGNVATTP
                     NNNNGFGGLVGGNFKTGTTPSLVTHLGFFAVNFTDQTDTNAVLYGEHHVGIFSGDGKV
                     LYASVDIQSGTYGITNGYIWMPLNPPFVLASNTTYLLLAEGGADPWGNSYHISDMNPF
                     YIGSNITSFSAIYTSGTWPSAGTLFGGYGGQMYTAPNLAILAPTTASAFVAPTNAAQY
                     VGLNTTLTATVDGQAPVTVQWYKEPGTQLTGQTSQTLNLNNLALSDAGNYYVIANNPG
                     GPVQSADAVVNVLGDAPVITQDVQSQTVYVHSTVLFPAGINGRPPLSYQWKFNGNPIS
                     GATASTLTLSDVSSANVGNYQLFVTNAYGATNSSVANLQVTYTVWGGYASGVMNSDLL
                     LYYRFSDISNYINSVSATATNQGSLGTGANGTYQGGWTQISGPTNILDLDESTNPAIA
                     GDGYDTDVLVPSGGSLSVTNCTIAAWVNDFYNPQGPNAAIFYHRQGSVFGFSVNPDGN
                     NIDQLRTTWNGSVHDSLIELPANQWVLVAMVVSPTNIAVYMNNGTTTQSTNLPSSNPP
                     ASLSGVSYVGYDTAGSSRRWLCGLDEVMVFNKALTAAQVNSLYLGVPQTATLTITPST
                     GGHMVVTWPGGTLQESTNVTGPWTPTSGASNGVYQVSPAGTMKFYRVKLQ"
     gene            complement(5835..>6684)
                     /locus_tag="VG754_07145"
     CDS             complement(5835..>6684)
                     /locus_tag="VG754_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWF19027.1"
                     /translation="FCGYWNIPFSDRRAAAFASCLRTQGYKLRVFPPVSGSVETGDGL
                     SRLPSDIQAVERRGLGRERAIAAWLRKQPRPLALFACNDVCGQQVLNACREHDIKVPD
                     EVAVLGVDNDDVLCNLCEPPLSSIEPNTERLGMEAAALLDAMINGKRINSGTVQIRPL
                     RVVERASTDVVAIEDPVTVQAVRYIRDRVGEGISVKDVLASVGRSRTDLEQRFRRWLK
                     SSIRVEIIRRRIDRACALLRQTDLGLDDIARQTGCSTTAHLCRLFQNRLQQTPTEYRH
                     AQKSRK"
BASE COUNT         1439 a         1856 c         1876 g         1513 t
ORIGIN      
        1 cgggtcgcgc cgcaacgctt ccagccagta gggtgttgga caccgtgtgg catgacgata
       61 ttgatcgagg tgaaggccgg tgatcaacag ttcatccgcg gtggcaatgt ccttcggcaa
      121 aggcggctcg gtcgcaggca acggcgcttt cccgcgaagc ggggttgaag gctgataccc
      181 gatgatctcg cggtcatcgg cgtccagcac gcgcaggcga agctcggttt ccaaaactcc
      241 ttcaggcaat tcgatttctt caaccaaagg cttttcaggc gtaagttcac aatcacgcgc
      301 aaacaccatt tcgccgcgcg ctgacaattg gaccttcagc gaatttcctg gagcagtcac
      361 gcagacgccg atgcgggctt tctgcttcac aaccttgaga ctgatcgcgg cctcaagatt
      421 ggccatctgc gccgggccga ttttttggat gggataccaa tactggctcc atgcccgcgt
      481 ctctcccggt tgaagaaaac tgaaatccgg ctggttgtcc gtgaagacgc ccgccatgag
      541 ttcgatgtaa ggcgcgtatt cgccgttttc atcgggctcg gtcaggtttc ggtcccacgc
      601 atagccgaac tcatgattgc cccacgtcca ttgttttttg cccggggaaa tatggtgatc
      661 ggcgatgtga acaatgcccg cctgcctttt gtaatcgtaa ccgccaaaaa aatcctcctt
      721 gctgccgacg cacatgtaac tgcacggcgt gggaatattc gcatagaacg aaaggtcgtt
      781 cggctgataa caaggcatgt ccgggacatt gccggacgaa tcattttttt cccggcaatg
      841 cggcgggaca aattgcctcg gcgcttcgcc ctttggcacc ccgtcgcgcg cgcgttgctt
      901 gtaatcaact ccgtaatagt gcccttgcgc caatggatat gcgctcatcg aacggcgcgc
      961 atggtccgcc acatattgaa cgtcgggtgg aaagaaactt tggtacgctt cgtggacgcg
     1021 cgttgccacg ttcgcccacc agagaaaagt ttgcgcaaat tcagtccgat tgtacgcgcg
     1081 gactttcaac tcgacgtaag cctttcccgg atgcaggcaa accccgtgca ttcccttcat
     1141 gcgcgccatc gggtcgtggt cgctgcacca agccgttgcc gaaccgtccg cgtgtttttc
     1201 aatttcggaa tcggtgggca ggaaagtcgc cggacgatga tgctgcggcc agttgaactc
     1261 gatgccgcct gaaacccatg gccccgccaa tccgacgagg gcgggcttga tgacccgctg
     1321 gttgtaaatc aaatcgtagc cgttggtctt gtcgaggatt gcgtggatgc gcccgccgat
     1381 ctccggcaaa atcatgactc gcaggaactc gttttccagc cagatcacct tccatttgcg
     1441 gtccacggga gtctccgcaa tccggtcggt aaagggcagg ggatagaccc ggccgctgct
     1501 gccttgataa acccgctttt ccaggaacat tggatgcacc gatggccgcg ctggcagata
     1561 agtcggcaga gtcacatctt cctgccgtac ggaaacggga ccgggtccag cagagatttt
     1621 ttccgcgagc tgcctttttt gactgcgtgt ttgagatgca gcttttccgg cccgaaaccc
     1681 gttttttccg ggcatggacg aatgaatact catgctgatt gaaatctttt accaatagcc
     1741 tcgcaatgcg ggatcaacgg tagcccccca catgctgttt gtcgagctat cgcgttcggc
     1801 cgcggtaaag agataaccgg agacgtggct gtccgcgtag aggatgttga aaaggccgac
     1861 accgtttacg gcgtgccagg cggaaaccat gtccaacggg cggtcgggag cgccgggcca
     1921 gtcaacgagg atgatcttgg tcgaaatctt ttcaaccatc tctgtccgtt tcatggccgg
     1981 agttgaaccg gaactgttgg tgcctccgcc gatggattct atcccgtaat aggaccatcc
     2041 gtaagttcca ttctggccgc cgctggcgga aatcaatccg ggttggcgcc acggcatcag
     2101 ataactgttg ccccaatcgg caaaacaagt ctgggacggt gtccaattca tggtgcctcc
     2161 actcgcgccc ggatctccct tgtcacccgg acagtagcaa gtattcacat ttttcaggta
     2221 tgaattaagc gggcggaggc catcgggata ttggaagcca tattcggctt gcggctggcc
     2281 tgaacctttt tgccccgccc aacatcccca atcctgatag accggatagt attcaccgaa
     2341 atcatcggcg tacatcgtca atgcaatccc aagctgccgc tcgttgctga cgcacttgat
     2401 ccggatggcc cgcacttttg cggcgcgcag ggccggcaac agcagggctg caagaatcgc
     2461 gatgattgcg atcaccacca gcaactctat caaagtgaac ccgggcgcgt tccccttcct
     2521 taacgcgccc gggttgcccg caaacaaaac agatttcttc atttttactg caattttacg
     2581 cggtagaact tcattgttcc cgcgggagaa acttgataaa cgccattgga cgctccggat
     2641 gtcggcgtcc acggtcccgt cacattcgtg gattcctgca gcgtccctcc cggccaggtg
     2701 acaaccatgt gccccccggt tgagggcgtg attgtcagcg tcgccgtttg cggcacgccg
     2761 agataaagcg aattgacctg cgctgcggtc aaggctttgt tgaacaccat cacttcatcg
     2821 agaccgcaaa gccagcggcg cgatgagcct gccgtgtcat acccgacata gctcacaccg
     2881 ctgagtgacg ccggaggatt gctggaaggg aggttggtgc tctgcgtggt cgttccattg
     2941 ttcatataga cggcgatgtt ggtcggactt accaccatgg cgacgagcac ccattgattt
     3001 gccggcagct ctatcagact gtcatggaca ctcccgttcc atgtcgtccg cagttgatcg
     3061 atgttgttcc catccgggtt taccgagaaa ccgaacacgc tcccttgccg gtggtagaat
     3121 atcgcggcgt tcggtccttg cggattataa aagtcgttga cccaagccgc gatcgtgcaa
     3181 ttggtgacag agagactgcc gcccgaaggc acgagcacat cagtgtcata cccgtccccg
     3241 gcaatggcgg gattggtgga ctcgtccagg tcgaggatgt tcgttggccc tgaaatctgg
     3301 gtccagcctc cttggtaagt cccattggcg ccggtgccaa ggctgccctg gttggtggcc
     3361 gtcgcgctga cgctgttgat ataattcgag atgtcgctga accggtaata aagcaacagg
     3421 tccgaattca tcacgcccga tgcgtaaccg ccccagactg tataggtgac ttgcagattc
     3481 gccactgagc tgttcgttgc cccgtaagcg ttcgtcacga ataattgata attgccaaca
     3541 ttggcggagg acacgtcgct cagtgtcaac gtgctcgcgg tcgcgccgga aatcggattt
     3601 ccattgaact tccattgata gctcaatggc ggcctgccgt tgatgccggc ggggaacagc
     3661 accgtcgaat gaacataaac cgtctgcgat tgaacgtctt gggtgatgac aggcgcgtcg
     3721 ccaagtacgt tcaccacggc atcggcgctt tggaccggac cgccgggatt gtttgcgatc
     3781 acatagtaat ttcccgcatc gcttaaggcg agattgttca ggttcaaggt ctggctggtt
     3841 tgccccgtta actgggtgcc cggttcctta taccactgaa cagtcacggg agcctgtcca
     3901 tccacggtgg cagtcaatgt cgtatttaat ccgacatatt gcgccgcgtt ggtcggggca
     3961 acaaaggccg atgccgttgt gggcgcgagg attgccagat tgggcgcggt atacatctgc
     4021 ccgccatatc cgccaaaaag ggtcccggca gatggccagg ttccgctcgt atagattgcg
     4081 gaaaagcttg ttatgttgct gccaatgtaa aacggattca tatcggagat gtgatacgag
     4141 ttgccccaag gatctgcacc accctcagcc agcaatagat aggtggtatt gctcgcaagc
     4201 acaaaaggcg ggttcaacgg catccatata tatccattcg tgattccata agtgccggac
     4261 tgaatatcca ccgacgcata caaaaccttg ccgtcgccgc tgaaaatgcc cacgtggtgc
     4321 tcaccgtaaa gaaccgcgtt ggtatcagtt tggtccgtga aattgacggc aaaaaatccc
     4381 agatgggtca ccagcgacgg cgtggtgcct gttttaaaat taccacccac aagtcccccg
     4441 aaaccattgt tattgttggg ggtcgtagcg acgttcccat gcaagtattc tgttggaaac
     4501 gcaaaatttg caaccactgt caatgtcgca ttggctgtat tggtgctcga gatgttattt
     4561 gacaccaagc aggaataaac atctccgttg ttggtcgatg cagccacgaa agagagcgag
     4621 ctgttggtcg cgcccggtat ggcggtgctg ttgcgcgacc attgataagc aatcaccggt
     4681 gtgcctgtgg ctgttaccga aaaggctgcc gggtaattcg caaacactgt gagattggtc
     4741 ggctgtttgg agattgaaac cggggtgctc gtgatcaaaa ccgtcacgtt tgaactctgc
     4801 tcgcctccga gcacgttgct cgcggtcaga aaataagtgc cgctgttgtt cgtggcggca
     4861 ttgggaatgg tcagggtcgc gttcgtctgg ctggtcaaaa gtgtatttgg ggccagatac
     4921 cactgatagg ttattggcgg ctggccgctg gcgaagccca gcaccgaaag cgtctgcccg
     4981 gtgccgattg aaatattggt tgtttgcact cccacccgcg cttgatctat ctcgatatat
     5041 cccagagtga cattgccgta ggcgtcgttg gtgctgttct gagtgaaccc agatggcggc
     5101 caagcgatgg gaacgccggg ggcatagccg gccccataca ccgcagtgcg gctgtctgtc
     5161 gtggtcgagc caacaaaaaa tgtattccaa gttggaataa aggtggtctg ccatgtgtcg
     5221 ccatccgcat tgctaaccac gccacccacg atataagagg tgttactgct caagagcacc
     5281 ggtgggtcca acggcaccca gcggaatcca ttggtcagat atgcatcggt gccagagggc
     5341 acaaccacct ggcccaaaac agttaaactc gtggtaaata cgcccgcttg atgacttacg
     5401 gccagtccgt cattgttgat gtcaaagact cccaaatgcg acacgatgac attgctggag
     5461 cccacttgaa actgggttcc gacgacccca ctgtaatcgt cccgcgccgc attcgtcggg
     5521 gtggcataca gcatttgagt gacttgcgct tgagatgaaa cggcaacggc aaacagaatg
     5581 cctgcggcca taatcgcgcg cagggacagg aaggtggttc ggtttgtgtt catggaatgt
     5641 tcgcttgttt agcgctggcg aagggtcgcc cgcacggggt tggttgctga tggatcatgt
     5701 attgaactct ggcttgggtc ccaaggtcca gtctattgtc aattttgaca aaatggttat
     5761 caattttaac aataagtgta ggagagagga tgagaaatac tttgactcaa gacttcaccc
     5821 cccgaatgac tcacttattt ccggctcttt tgggcgtgcc ggtactcagt gggagtctgc
     5881 tgaagacggt tttgaaacaa acggcaaagg tgcgcagtgg tgctgcaacc agtctgacgg
     5941 gcgatgtcat ccaagcccaa atcggtctga cgcaacaggg cgcaggcgcg atcaatgcgg
     6001 cggcggatga tttcgacccg gatgctgctc ttaagccagc ggcgaaaacg ttgttccaaa
     6061 tcggtccgag aacggccaac gcttgccagc acgtccttca cgctgatgcc ttctccaacg
     6121 cgatcacgga tgtagcggac ggcttgcacg gtgacggggt cttcgattgc cacgacatcg
     6181 gttgacgcgc gctcgacaac gcgcagcggg cggatttgca ccgtaccgct attgatgcgc
     6241 ttgccattaa tcatggcatc gagcaacgct gccgcttcca tgccgaggcg ttcggtgttc
     6301 ggctcgatgc tgctcaaggg tggttcacac aaattgcaaa gcacgtcgtc attgtccact
     6361 cccaggaccg cgacttcatc cggcactttg atgtcatgtt cgcggcaggc attgaggacc
     6421 tgctggccgc acacatcatt gcaggcgaac aacgccagcg ggcgaggttg tttgcgcaac
     6481 catgccgcga ttgcccgctc tcttcccaat ccgcgccgct cgaccgcctg gatgtccgat
     6541 ggcaatcggg acaaaccgtc gcccgtttca acggaacccg aaaccggcgg aaacacgcgc
     6601 agcttgtaac cttgagtgcg caaacaactg gcgaacgcag cggcccgcct gtccgagaac
     6661 gggatgttcc aatagccgca gaaa
//
LOCUS       DASZMM010000300         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2591912, whole genome shotgun sequence.
ACCESSION   DASZMM010000300 DASZMM010000000
VERSION     DASZMM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591912"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1679
                     /locus_tag="VG754_07150"
     CDS             72..1679
                     /locus_tag="VG754_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWF19028.1"
                     /translation="MTTTVSRFEGLLDRHFETLLAEQPVLAAYAGLSEGRGQLGKATH
                     TFEKRWQTMRRRTLTTLDSLSPRELSNEQQLDRLAFRSQLLRECEDFERGRHTLNPDA
                     LDHVLNALLFELQRAEDEPQAVARNLRSLLKETPRYLSEAAALIDRPERVWRKIMEQT
                     AAGAGSLFDAVGVFLTRNGQRDADASLIATAQKAFADYQKKVCARPLAREGSFAIGVP
                     TMQRRVRDQLGLDYSLGEIEALALSEVERIGNRLSAACWKFGKGKSVEEIIAEKRAEW
                     NPGGDLIGLYRAETKRVADSFRAAGAVTFPAGDVLEVRSVPEFMRHLFPTAAYSSPGP
                     FEKRQRGIFWVNDLSVTKKTDAEKRAEQQQHFGLTLTCAHEAYPGHHLQFVTANQHPR
                     KWRRMFAHAVFFEGWTLWCEQMMVDLKIDRSPWLQVQQLHDALWRCHRILVDLRLQTG
                     RYTYEQGVKHMEKNLGFTRARAQADVNWYTASPTVPMSYWLGRLENERLRQRLIVGRG
                     WSLQKFNDWLLSFGTIPQAWIEKYGLD"
     gene            complement(1681..1944)
                     /locus_tag="VG754_07155"
     CDS             complement(1681..1944)
                     /locus_tag="VG754_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19029.1"
                     /translation="MFKQLFDLGRQALFLLRDVEQNKQEIAKVRRELDETNSLVLQLA
                     HELQRISEREVHERERLELRLENALLRFEKAISSAKPVKKRRK"
     gene            complement(1925..2104)
                     /locus_tag="VG754_07160"
     CDS             complement(1925..2104)
                     /locus_tag="VG754_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19030.1"
                     /translation="METKQTPKYLTALDECLKEMSVIRKNMKKQDAEIRRLESSTRRK
                     LDETKAIISRVQAII"
     gene            2223..>3694
                     /locus_tag="VG754_07165"
     CDS             2223..>3694
                     /locus_tag="VG754_07165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HWF19031.1"
                     /translation="MNLLPFENLPIYKPRSFVPAKVDWGDWNQIAPLYDQLEARAPQC
                     KTVEEVERWLLDWSELNAAIDQDHSERYIAMTCHTDNPDAEKAYLHVVEHIQPQLKPR
                     EFKLAQIYMEHPLRSRFSKSIRYEVFDRNTALQVELFRPENVPLETQEAKLSQQYQKL
                     SGSLTVQFRGEEKTLAQMARYLEEPDRALRQESWELVANRRLQEVDKIEAIFDEQVKI
                     REQIARNAGFKNYIDYAFRSRRRFDYTPEDCLQFHDAIEKELMPIVRELQAKRREQLK
                     LPALRPWDLGVDPLNRPPLRPFEKVEGMVSNTQQIFNQLDQTLGAEFQQMQDLRLLDL
                     ANRKGKAPGGYQSTLTEARLPFIFMNAVGVQRDVETILHEAGHAFHALASRGEDLYAY
                     RDAPIEFCEVASMSMELLGNEFIEKFYAPPEAKRARHDHLEGIVKIFPWIATVDAFQH
                     WIYSHPGHSRADRRAAWEKLMDRFGGDVDWSGYENTRGHL"
BASE COUNT          817 a         1002 c         1036 g          839 t
ORIGIN      
        1 agaagtttgg attacaaaca agccttctac gattgccaat gcccttatga tatggctttc
       61 tttctcctcg aatgaccacc acagtgtcgc gatttgaagg cctgcttgac cgtcattttg
      121 aaactctcct tgcagagcag cctgttctcg cggcttatgc aggtctttcc gaggggcgcg
      181 gccaattggg caaggcgact cacacatttg aaaaacgctg gcaaacaatg cgccggcgca
      241 cgctcaccac tttggattcg ctttcgcctc gcgaattgtc caatgaacag cagcttgacc
      301 ggctcgcctt ccggagccaa ctgttgcgcg agtgcgaaga cttcgaacgt ggtcgtcaca
      361 cgctgaatcc cgatgcactg gatcatgtgc tgaacgcgct gctcttcgaa ttgcaacgcg
      421 ccgaagacga gccgcaggca gtcgcgcgca atttgcgttc gctgctcaag gaaacgccgc
      481 gttatctgag cgaggccgcc gcgctgattg accgccccga acgtgtctgg cgcaaaatca
      541 tggaacaaac cgccgccggc gcaggttcgc tttttgatgc ggtcggcgtt tttctcactc
      601 gcaacggaca acgcgatgcg gatgccagct tgattgccac cgcgcaaaag gcatttgcgg
      661 attatcagaa gaaagtttgc gcgcgtccgc tcgcgcgtga agggtcgttt gccatcgggg
      721 tgccgaccat gcaacggcgc gtgcgcgatc aacttggatt ggattactcc ctcggcgaaa
      781 tcgaagcgct tgcgttgtcc gaggtcgaac gcatcggcaa ccgtttgagc gcggcctgct
      841 ggaaattcgg caaggggaaa tcggtcgagg aaatcattgc cgagaagcgc gctgaatgga
      901 atccgggagg ggatttgatc gggctttatc gggctgaaac caaacgcgtc gcggactcgt
      961 ttcgcgcggc gggagcggtg acttttccag caggcgatgt tctcgaagtg cgttccgtgc
     1021 cggagttcat gcggcacctg tttcccactg ccgcttattc gagtcccggc ccctttgaga
     1081 aacggcagcg aggcattttc tgggtcaacg atttgagcgt gaccaaaaag accgacgccg
     1141 aaaaacgcgc tgaacagcag cagcatttcg gactgacgct cacctgcgcg cacgaagcgt
     1201 atcccggtca tcacctgcaa tttgtcaccg ccaaccagca tccgcgcaaa tggcggcgca
     1261 tgtttgccca cgcggttttc ttcgaaggct ggacgttgtg gtgcgagcag atgatggtgg
     1321 atttgaagat tgaccgctcg ccgtggctgc aggtccagca gcttcacgac gcgctctggc
     1381 gttgccatcg catcctggtt gatttgcgtt tgcagaccgg ccgttacacc tacgaacaag
     1441 gggtcaagca catggagaaa aatctcggct tcacccgcgc ccgcgcgcaa gccgacgtga
     1501 attggtacac cgcatcgccc accgtgccga tgagctactg gctggggcga ttggaaaatg
     1561 aacgattgcg tcagcggctg atcgttggcc gtggctggag tctgcaaaag ttcaatgact
     1621 ggctcctgag cttcggcacc attccgcagg cgtggattga aaagtatggg ctggattgaa
     1681 ttatttcctg cgcttcttca caggtttggc cgaagaaatt gccttttcga agcgaagcag
     1741 ggcattttcc agacgaagct ccaatctctc gcgttcatga acttcgcgtt cgctgattcg
     1801 ctgcagttca tgggcaagct gcaggacaag agagttcgtt tcgtcaagtt cacgtcgaac
     1861 cttggcgatt tcctgcttgt tttgctcgac atcgcgaagc aggaataaag cctgccgccc
     1921 caggtcaaat aattgcttga acacgggaaa tgatggcctt tgtctcgtcg agtttgcggc
     1981 gtgtggaact ctcaaggcgc ctaatttccg catcctgttt tttcatattc ttgcgaatga
     2041 cgctcatttc cttgaggcac tcatccaacg ccgtcaaata tttcggcgtc tgcttggttt
     2101 ccaaagcaac tgttcccgtt ttcatgcact cagtttaaag gatgtgcggc ccgatatcaa
     2161 tccattgtta acgatatata aatctggttt ccaatctggg atcttggtct atcttctgga
     2221 ttatgaactt gttgcctttt gaaaatttgc ctatctacaa accgcgcagc tttgtgcccg
     2281 cgaaagttga ttggggggat tggaaccaga tcgcgccgtt atacgaccag ttggaagccc
     2341 gcgctccgca atgcaaaact gtcgaagaag tcgaacgctg gcttttggac tggagcgaat
     2401 tgaatgccgc gattgatcag gaccattccg agcgctatat cgccatgacc tgccacacgg
     2461 acaatcccga tgctgagaag gcttacttgc acgtggtcga gcacatccag ccgcaactca
     2521 agccgcgcga attcaagctc gcgcagattt acatggagca tccgttgcgc agccggtttt
     2581 ccaaaagcat ccgttacgaa gttttcgacc gcaacactgc gctgcaagtt gaattgttcc
     2641 ggcccgagaa cgtcccgctg gaaacgcagg aggccaagtt gagccagcaa taccaaaaat
     2701 tgagcggttc gctgactgtt cagttccgcg gcgaagaaaa aacgctcgcg cagatggcgc
     2761 gttacctgga ggagccggac cgcgcgttgc ggcaggaaag ttgggaactg gtcgcaaacc
     2821 ggcgtttgca ggaagtggac aaaatcgaag ccatcttcga cgagcaggtg aaaattcgcg
     2881 aacagatcgc caggaacgcc ggatttaaaa actacatcga ttacgccttc cgcagccggc
     2941 ggcgctttga ttacacgccg gaagattgcc ttcaatttca cgacgccatc gagaaggaac
     3001 tcatgcccat cgtgcgcgaa ttgcaggcga aacggcgtga gcaattgaaa ttgcccgcgt
     3061 tgcgtccttg ggatctcgga gtggatccgc taaatcgtcc gccgctgcgc ccgtttgaaa
     3121 aagtggaagg aatggtttcc aacacgcagc agattttcaa tcaactcgac cagactttgg
     3181 gcgcggaatt ccagcagatg caggacttgc gtctgcttga cctcgccaat cgcaagggca
     3241 aggcgcccgg cggttatcaa tccacgctca ctgaggcgcg gttgccgttt attttcatga
     3301 acgcggtcgg cgtgcagcgc gatgtggaaa ccattcttca cgaagccggg cacgcgtttc
     3361 acgcgcttgc cagccgcggt gaagatttgt acgcgtatcg cgatgcgccc attgaatttt
     3421 gcgaagttgc ctcgatgagc atggaactgc ttggcaatga attcatcgaa aaattttacg
     3481 cgccgccgga agccaaacgc gcccggcacg atcaccttga aggcatcgtg aaaatttttc
     3541 cgtggattgc aaccgtcgat gcgttccagc actggattta ttcgcatccc ggccacagcc
     3601 gcgccgaccg cagggccgcg tgggaaaaat tgatggatcg tttcggcggc gacgtggatt
     3661 ggagcggata tgaaaatacg cgcgggcatt tgtg
//
LOCUS       DASZMM010000301         9121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2036114, whole genome shotgun sequence.
ACCESSION   DASZMM010000301 DASZMM010000000
VERSION     DASZMM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036114"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VG754_07170"
     CDS             <1..585
                     /locus_tag="VG754_07170"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWF19032.1"
                     /translation="SQLTPDEIRFLQRSAEYNQTDIQLGQTMVQKCDAPSLKTYGQRL
                     ADDHTLANQQLQSIIAQTGATVPTAPNPDQQQMLDRLNGLSGTDIDRAAIDDSMRSQE
                     RQINLDMQAANNSQNPEVKAYAQQNLTINQDELNQCRGLNVPAPTAAPVYSTPPATTV
                     APVIVPPPPPAVEPVPPPPSERPAGPGISPQQPQ"
     gene            complement(667..1233)
                     /locus_tag="VG754_07175"
     CDS             complement(667..1233)
                     /locus_tag="VG754_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19033.1"
                     /translation="MHTPQLTPDFLVEHRLQGYERVIPMKLEPPDTHHVNAAQGWLGL
                     GNANEANAELDKIPDALQSHPDVLELRWQVYANARKWQQCVEVGARLVELAPRKPQGW
                     IHRSYALHELKQTRDALEALLPAVTLFPKEWLIQYNLACYCCRLDEPERALQFLRQAF
                     KLGDAKEVKPMALTDPDLREVWKKIETM"
     gene            1228..1533
                     /locus_tag="VG754_07180"
     CDS             1228..1533
                     /locus_tag="VG754_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19034.1"
                     /translation="MHLIQILLPVYDNHGKAFPREHYERVKAELSARFSGLTAYVHSP
                     AEGIWTKGKATQREDIVVFEVMAPKVDRKWWRHYRADLEQRFRQETVVIRRQSITLL"
     gene            complement(1556..2116)
                     /locus_tag="VG754_07185"
     CDS             complement(1556..2116)
                     /locus_tag="VG754_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWF19035.1"
                     /translation="MKRAFEENFEPATLAVPVSKHDHIQGRYDAPLTLVEYGDYQCPA
                     CGAAYPVVKTVQSTLGDRLRFVFRNFPLTNIHPNAEHAAEAAEAADEQGRFWEMHDTL
                     YENQDALEDENLMQYALDLGLDARRLVREVQNGAHSDRIRKDFQSGIRSGVNGTPTFF
                     INGVRHNGPWDAETLVAELTQSAVAS"
     gene            complement(2113..2643)
                     /locus_tag="VG754_07190"
     CDS             complement(2113..2643)
                     /locus_tag="VG754_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWF19036.1"
                     /translation="MYPGQHRPTALPPGTLAPDFSLRSTPDQSVRLTDFRGQPVVLAF
                     YPADWSPVCGDQMALYNEILPEFQNLGAELMGISVDGSWCHAAFARDRKLHFPLLADF
                     EPKGEVARNYGVYRQGEGVCERALFVIDAEGNIHWSYVSPIGVNPGAAGILSALEDLQ
                     NKEKPKTEEKKEEVAK"
     gene            complement(2811..4979)
                     /locus_tag="VG754_07195"
     CDS             complement(2811..4979)
                     /locus_tag="VG754_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWF19037.1"
                     /translation="MPTKRALIFAMMLFALGMAAGTYAQSQTNSTVTTSNPASPPSHS
                     HTNRLAREKSPYLLQHQHNPVDWYAWGDEAFARARNENKPIFLSIGYSTCHWCHVMER
                     ESFEDEKLAKYLNEHFVSIKVDREERPDVDKIYMTFVQSTTGGGGWPLNCFLTPDLKP
                     FFGGTYFPPESKYGRPSFLELLQQITKLWQTRHGDVTNSAEELHERLAATSDKPSPGE
                     LALTPEVLTNAATLFKRTFDPQNGGFGDAPKFPQPSQPAFLLRYGVRFKDDEAVKMVL
                     QTCERMAAGGIHDQIGGGFARYSVDAQWLVPHFEKMLYDNAQLVNLYLDAWLVSRDAR
                     DADTARDILRYVLRDMTHPGGGFYSAEDADSEGKEGKFYCWTREELSKLLTPEEFNVA
                     TRYFGITEQGNFVDHSDPQPLRGQNVLSIVQPKLTKEDEALLKSAKEKMFAARGKRVR
                     PHLDDKVLASWNGLMLGAMARAYAVLGDETYRVAAEKNLSFLRDKLWDAKTATLHHRW
                     RDGERDSVQLQQAYAYLLAGVIDLYEATLDPKHLDFAIALADSMTAKFEDKQGGGFWQ
                     SAADAPDLIMRVKEDYDGAEPSGNSVAALALLKLAAITGRDDYKKSAEGALRFASERL
                     QKIPQAVPCMLLALDYSLEEPKRVVIAGDPAMPDTKALLHAAHSIYQPGKVVSGIAGA
                     VEPFAKTLPAKEGAVVYLCTGNACQPPTKDAAQLKEMLAK"
     gene            complement(5259..6836)
                     /locus_tag="VG754_07200"
     CDS             complement(5259..6836)
                     /locus_tag="VG754_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWF19038.1"
                     /translation="MGNDVGGDLAKLQSVPRETARWQKAQNQLMSGRYGHALAGYRSL
                     VESHPRVAQLWFELGIAAGGELDFEQAHNAFQRAITLASNDVSLLVLIGQQYQRLRRL
                     DIARDCYRRAVLADPGSSHARLSLAAWYERERRMDEAWNCVEESLAHTPKDPQALYFR
                     AFLLHRKGMEREAETALRELVASNAPQDAAVKHSIRHLLAMVLDELGQYDEAMRWLRE
                     AKTLLRQSVDAPALERIYDQTDRQRRELLKALTPETIRRWRDESTKESEEHKLAFLGG
                     HPRSGTTLLEQVLGAHPEIIAFDEPEAFTQEVLNEVAPPQSARGLTLDGLDGLSAASR
                     AALSKRYFKSLLREVNSPPAAGQVLLDKNPSPTASLHLWLRVFPKLKVIIPLRDPRDV
                     VVSCFFQNLTVTSSSVNFLSLERTARHFGDTMDVWLRMKELGGFDWIESRYEDMVQNL
                     EGEGRRVTEFLGLKWEPSQMHYREAAHHPFVFAPTYHDVTKPVHNRAVRRWEHYADAL
                     APVMEKLAPYCRAFGYE"
     gene            7085..7169
                     /locus_tag="VG754_07205"
     tRNA            7085..7169
                     /locus_tag="VG754_07205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7119..7121,aa:Leu,seq:gag)
     gene            7204..7605
                     /locus_tag="VG754_07210"
     CDS             7204..7605
                     /locus_tag="VG754_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19039.1"
                     /translation="MHRFCTDFAQNPRESVAPKMRFPKTIRHRKGIVKIYGKRKNYPY
                     YRIAYWLEGKRHLASFTRYSDALETPKKKAKEISEGHAGVALSSMQARDALNAFQILD
                     SHRESGVRLTLTNIVSDYVELSKKIGKAYVP"
     gene            7693..8334
                     /locus_tag="VG754_07215"
     CDS             7693..8334
                     /locus_tag="VG754_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19040.1"
                     /translation="MKTNKQYIFFILVVIACAFGCKTRPESYVVRIAETESKKWRLAL
                     NGASVGGDVSNSDFTNQLLQLHLSKNDIVIFSQKPATEARQIWSSWIWAVNYCASNKV
                     ASYLYGDPQSTQELFSIPIYNWTAPFDDPGSLTKSSFFYNGKSLGHGMEGFKKMVDSV
                     ANGKAHKILILGSLYNRDSQFGSDERPYEQLQNLLDAGLKKSGSQLISVSQLP"
     gene            complement(8660..>9121)
                     /locus_tag="VG754_07220"
     CDS             complement(8660..>9121)
                     /locus_tag="VG754_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19041.1"
                     /translation="LAGLLFVALSLNRERITGQENRILLRLAQRSFGDFLYALFIALM
                     FLIPCFRSRDLVIPLVILLAFRGQWLVRAFYRTRKMTREKRAALKVAREYLFQTISWL
                     ALLAATIEIYRGEMAATYLLVPVVALLLYNATRNAWQLLIMEKQIEQKENS"
BASE COUNT         2062 a         2527 c         2464 g         2068 t
ORIGIN      
        1 agccagctaa caccggatga aatccgtttc ctgcaacgct ccgctgaata caatcaaacc
       61 gacatccaac tcggccagac catggtccag aaatgcgatg ctccgagttt gaagacttac
      121 ggtcagcggc tggcggatga ccacacgctg gccaatcagc aattgcaaag catcatcgcg
      181 cagaccggcg ccacagtgcc gaccgcgccg aacccggacc agcaacagat gctggaccgg
      241 ttgaacggcc tcagtggaac ggacattgac cgcgcggcca ttgatgattc gatgcgttcg
      301 caagaacgcc agataaatct cgacatgcaa gctgcgaata attcccaaaa ccccgaggtg
      361 aaagcctacg cgcaacaaaa tctgaccatc aatcaggacg aactgaatca atgccgcgga
      421 ttgaacgtcc cggcccccac agccgcgccg gtatattcga cgccgccagc cacaacggtt
      481 gcgcctgtga tagttcctcc gccgccgccc gcggttgagc cggttcctcc tccgccttca
      541 gaaaggccag caggcccggg aatttctcca cagcaaccgc agtagggtaa gctcagacag
      601 tgcccggatt gccgggcgaa ttcgggccgg acctcgggat ttatgagtgg aaattccggg
      661 tccggctcac atcgtttcga tttttttcca aacctcgcgc aagtcgggat cggtgagcgc
      721 catgggtttg acttccttcg cgtcgcccag tttgaaggcc tgccgcaaaa attgcagcgc
      781 gcgttcaggc tcgtccaacc ggcagcagta acacgcgaga ttatattgga tgagccattc
      841 tttgggaaac aacgtcaccg ccggcaacaa tgcttccaat gcatcgcggg tctgtttcaa
      901 ttcgtgcaat gcatacgaac gatgtatcca gccttgcggt ttcctgggcg caagttccac
      961 gaggcgagcg cccacctcga cgcattgctg ccatttgcgc gcgttggcat aaacctgcca
     1021 gcgcagttcc agcacgtccg gatgcgactg caatgcatcg ggaattttgt ccagctcggc
     1081 gtttgcctca ttggcatttc ccaggccgag ccagccttgc gcggcgttca cgtgatgcgt
     1141 gtcaggcggt tccaatttca tcggtatcac gcgttcatac ccttgcaacc ggtgttctac
     1201 aaggaaatca ggcgtaagtt gaggtgtatg catctgatcc agattttgct tccggtttac
     1261 gacaatcatg ggaaagcctt cccgcgcgaa cattatgaac gcgtcaaggc cgaattgtcc
     1321 gcaagattca gcggcttgac cgcttacgtt cactcgcccg cggaaggcat ctggaccaag
     1381 ggcaaagcga cccaacgcga agacatcgtc gtctttgaag tcatggctcc gaaggtggac
     1441 cgcaaatggt ggcggcatta ccgcgcagat ctggaacaac ggttccgtca ggaaaccgtt
     1501 gtgattcgcc ggcaaagcat cacgcttctt tgatgatgag gcgatttcgg gaacgtcagg
     1561 atgcgaccgc cgattgagta agttccgcca ccagtgtctc ggcgtcccaa gggccgttat
     1621 gacgaactcc attgatgaag aacgtcggcg tgccgttcac accgctgcgg attccacttt
     1681 ggaaatcctt gcggatgcgg tcgctatgag cgccgttttg gacttcgcgg accagccggc
     1741 gcgcgtccaa tcccaagtcc agggcgtatt gcatcagatt ttcatcttcc aaggcgtcct
     1801 ggttttcgta gagcgtgtca tgcatttccc aaaagcggcc ttgctcgtcg gcagcttcgg
     1861 cagcctcggc ggcgtgctcg gcgttgggat ggatattggt caacggaaaa ttgcggaaca
     1921 cgaatcgcaa ccggtctccc aaagtggatt gtaccgtttt cacgaccgga taagccgcgc
     1981 cgcaggcggg gcattgataa tcgccatatt caacaagtgt tagtggagca tcgtatctcc
     2041 cctggatgtg gtcatgtttg ctgacaggca cggcaagtgt ggctggttca aaattttcct
     2101 cgaatgctcg tttcattttg ccacctcttc tttcttctcc tctgttttcg gcttctcttt
     2161 gttttgcaaa tcttccaatg cggacaagat tcccgccgcg ccgggattca ccccgatggg
     2221 cgatacataa ctccagtgga tgttgccctc ggcatcaatc acgaatagcg cgcgttcgca
     2281 gacaccttcg ccctggcgat agacaccgta attccttgcc acttcccctt tcggctcgaa
     2341 atcggccagc aacggaaaat ggagcttgcg gtcgcgggcg aaagccgcgt gacaccacga
     2401 gccgtccacg gagatgccca taagttctgc gcccaaattt tggaactcag gcaggatttc
     2461 gttgtaaagc gccatctggt caccgcagac cggactccag tccgccggat aaaatgccag
     2521 cacgaccggc tggccgcgaa aatccgtcag gcggacagat tggtcggggg tgctgcgcag
     2581 tgaaaaatcc ggggcgagag tgccgggcgg cagcgcggtg gggcgatgct ggccggggta
     2641 catgcggttg taattttgtt gtgtttgttt gtccataaat aacctttcgt taaaaggtcc
     2701 cggaaggaac cgttacggcc actaacatca gcgcgattga agcagtcttc aaataaaaat
     2761 cgctgaagtt ttttatgggt tggatgctcc ccgccttggg ttaaacgtca ttatttcgcg
     2821 agcatttctt tcagttgtgc ggcgtctttt gtgggcggct ggcaggcgtt gccggtgcat
     2881 agatagacga ctgctccttc ctttgcgggc aaagtttttg cgaacggctc aaccgccccc
     2941 gcaatgcccg acactacttt tccaggttga tagattgagt gcgccgcgtg caacaacgcc
     3001 tttgtgtctg gcatggcggg gtccccggcg atgaccacgc gcttaggctc ttccaacgaa
     3061 taatccagcg ccagcagcat gcagggaacc gcctgcggaa ttttttgcaa ccgttccgac
     3121 gcgaatcgca acgcgccttc ggcggatttt ttgtaatcat cgcggcccgt gatggcggcg
     3181 agtttcagca atgcgagcgc ggccactgaa ttgccggaag gttccgcgcc atcgtaatcc
     3241 tccttcacgc gcatgatcaa atcgggcgcg tctgcggcgc tttgccaaaa accgccgcct
     3301 tgcttgtcct caaatttcgc ggtcatgctg tcggcaagcg cgatggcgaa atcgagatgt
     3361 ttcggatcca gcgttgcttc atacaaatca atcacgcccg cgagcagata cgcataagcc
     3421 tgttgcaatt gcacggaatc ccgctcgccg tcgcgccagc gatgatgaag cgtcgcggtc
     3481 ttcgcgtccc aaagtttgtc gcgcaaaaat gaaagatttt tttcggcggc aacgcgatac
     3541 gtttcatcgc cgagcacggc ataagcgcgc gccatcgctc cgagcatcag gccgttccac
     3601 gaagccagca ccttgtcgtc aagatgcgga cgaacccgtt tgccgcgggc ggcgaacatt
     3661 ttttccttcg ccgatttgag cagcgcttcg tcttctttcg tcaacttggg ttggacaatg
     3721 ctgagcacat tttggccgcg caacggttgc gggtcgctgt ggtccacaaa attgccctgc
     3781 tcggtgatcc caaaatagcg cgtggccaca ttgaattctt ccggcgtcag caatttggaa
     3841 agctcctcgc gcgtccagca ataaaatttg ccctccttgc cttcgctgtc ggcatcttcg
     3901 gcggaataaa atccgccgcc gggatgcgtc atgtcgcgca aaacataacg caagatgtcc
     3961 cgcgcggtat cggcgtcgcg cgcatcgcgg ctgacgagcc acgcatcaag atagagattc
     4021 accaattgcg cattgtcgta gagcattttt tcgaagtgcg ggacgagcca ttgggcatcg
     4081 acggaataac gcgcaaaacc gccgccaatc tggtcgtgaa ttccgccagc cgccatccgc
     4141 tcgcacgttt gcaaaaccat ttttaccgcc tcgtcatcct tgaaccgcac gccgtagcgc
     4201 agcagaaaag ccggctggct cggctgcgga aatttcggcg catcaccgaa accgccgttc
     4261 tgcgggtcga aggtgcgttt gaataaagtt gcggcattgg tcaggacttc cggcgtgagc
     4321 gccagttcgc cgggcgatgg cttgtctgaa gttgccgcaa gccgttcgtg caattcctcg
     4381 gcggaattcg tcacgtcgcc gtggcgcgtt tgccaaagtt ttgtgatctg ctgcaaaagt
     4441 tcgagaaaac ttgggcggcc gtatttgctt tcgggtggaa aataagttcc gccaaaaaat
     4501 ggtttcaaat caggcgtgag aaaacaattc aacggccagc cgccgccgcc cgtggtggat
     4561 tgcacgaagg tcatgtaaat cttgtccacg tccgggcgct cctcgcggtc caccttgatg
     4621 ctcacgaaat gttcgttgag atatttcgcc agtttctcgt cctcgaacga ttcgcgttcc
     4681 atcacatgac accaatggca ggtcgaataa ccgatgctca aaaatatcgg cttgttctca
     4741 ttgcgcgcgc gggcgaacgc ttcatctccc cacgcgtacc aatccaccgg gttatgctga
     4801 tgctggagca ggtaaggcga cttctcgcgc gcaagacggt tggtatgcga atgcgacggc
     4861 ggagaagctg gattggaagt ggtcacagtt gaattggttt gagattgagc atatgtcccg
     4921 gcggccattc ccagcgcgaa aagcatcatt gcgaaaatca acgcgcgttt cgttggcact
     4981 gccgcaccat aacccctgcc gccccttccg gcaatggcgc aaaaacgccc ttcacacgcc
     5041 tcgcggacct ccacgaacgg gaaccctgcc ggcgaaaagc gccatttttc aaggcaggaa
     5101 aggtggtcac cttcgagtgc tttttgaacc gctcaaaatg gctgttttcc gctcctgtcc
     5161 gtgtttcatc cgtgttcaat ccgtggctca actgcatcgt tccagtccat ttccatgaac
     5221 aggcatgttt tctacaaagc aagatgactt ctgggaacct actcgtaacc aaacgcccgg
     5281 caatagggcg ccaatttttc catcaccggc gcgagcgcgt cggcgtaatg ttcccagcgg
     5341 cgcacggcgc ggttgtgtac tggtttggtc acgtcgtgat acgtcggcgc gaaaacgaac
     5401 ggatggtgcg cggcttcgcg gtaatgcatc tggctcggtt cccatttcag gccgagaaat
     5461 tctgtcacgc gcctgccttc gccttccaaa ttttgcacca tgtcctcgta gcgcgactcg
     5521 atccaatcga acccgcccag ttctttcatc cgcagccaga cgtccatcgt gtcgccaaaa
     5581 tgccgcgctg tgcgctccaa gctcaagaaa ttcacgcttg atgaagtgac agttagattt
     5641 tgaaaaaagc aactgacgac cacatcgcgc gggtcgcgca acggaatgat gactttgagc
     5701 ttcggaaaga cacgcagcca aaggtgcagc gaggcggtcg gcgatggatt tttatcgaga
     5761 agcacctgac cggcggcagg cgggctgttc acttcgcgta aaagactttt gaaatagcgc
     5821 ttgctcaagg ccgcgcggct cgccgcgctc aatccgtcaa ggccgtccaa ggtcaatccg
     5881 cgggccgatt gcggcggggc gacttcgttc aacacctcct gcgtgaacgc ttccggctcg
     5941 tcgaaggcga tgatttccgg gtgcgcgccc aggacttgtt caagcaatgt tgtcccgctg
     6001 cgtggatgtc cgccgaggaa ggcaagcttg tgttcctcgg attctttcgt gctttcgtcg
     6061 cgccagcggc ggatggtttc cggcgtcaat gccttcagca actcgcgacg ttgccggtcg
     6121 gtttggtcgt aaatgcgttc caaggctggc gcgtccacgc tctgacgcag caatgttttg
     6181 gcttcgcgca accagcgcat cgcttcgtca tattgcccca attcgtcgag gaccatcgcg
     6241 agcagatgcc ggatggaatg tttcacggcg gcatcttgcg gagcgttgct ggcgaccaat
     6301 tcgcgcaacg ccgtttcggc ttcgcgctcc atgcccttgc gatgcagcag gaacgcgcgg
     6361 aaataaagcg cttgcgggtc ctttggcgtg tgcgcgagcg attcctcgac gcaattccac
     6421 gcttcatcca tgcggcgttc gcgttcgtac caggccgcca ggctgagccg cgcatggctt
     6481 gaaccggggt cggccagcac cgcgcgccga taacaatccc gcgcgatgtc cagccggcgc
     6541 aaacgttgat attgctggcc gatgagaacg agaagggaaa cgtcgttgga agcgagagtg
     6601 atggcgcgtt ggaaggcgtt gtgggcctgt tcgaaatcga gttcaccgcc cgcggcgatg
     6661 ccgagttcga accagagttg agcgacgcgg ggatgggatt cgacgagaga gcgatacccg
     6721 gccagggcat ggccgtaacg cccgctcatc aactggtttt gtgccttttg ccaacgggct
     6781 gtttcgcgcg gcacactttg cagcttcgcg agatcgccac ccacatcatt gcccatctaa
     6841 ctgtctttca tataatcaaa atcggacttg ctgttatacg cttgcagacc ggtgtcccgg
     6901 ccaagaggtt acaaaacgat gacaaaaaag tgcgtttggc ggcaattcct ttcgacgagg
     6961 cgaataacgg gaaaaaaggc aggcgatttc acccaaaaat caagggcgtg aaacatgaca
     7021 agcctgcaaa atgcccttgc gaatttggtt caagactgct tcattaggtg agccggtgcc
     7081 gagtgcgctt atggtggaat tggcagacac gctactttga ggtggtagtg gggcaacccg
     7141 tgcaggttca agtcctgcta agcgcaccaa cttttcctcg gaaactggtg agcagcttga
     7201 cttttgcaca gattttgcac agattttgca caaaatcccc gcgaatctgt tgcaccaaaa
     7261 atgcggtttc ccaagacaat tcgtcatcgc aagggcatcg tcaaaatcta cgggaagagg
     7321 aaaaactacc cgtattatcg catcgcatac tggttagagg gcaagcgtca cctcgccagt
     7381 tttaccagat acagcgacgc actggaaacg ccgaaaaaaa aggcaaagga aatttcagaa
     7441 ggccacgccg gcgttgcatt gtcgtctatg caggcacgtg acgcgctaaa cgcctttcaa
     7501 attcttgatt cgcatcggga gtcgggtgtg cggttgacac tgaccaatat tgtttctgat
     7561 tatgtggagc tttccaaaaa aattgggaaa gcatacgttc cgtgaagcag ttgagcgttt
     7621 tttgatatcc ggagtgcatg gaaaacctag gtggcgcaaa agcattaact ttccttcaac
     7681 agctctttag caatgaaaac aaacaaacaa tatatctttt ttattttggt tgtgatcgct
     7741 tgtgcctttg gatgcaaaac taggcctgaa tcgtacgttg tacgcattgc tgaaacagaa
     7801 tcaaaaaagt ggaggctggc gctaaacggg gcgtctgttg ggggggacgt ctccaattca
     7861 gactttacga atcaactgct tcaacttcat ctgagcaaga acgatattgt aatttttagc
     7921 caaaaacctg caactgaagc caggcagatt tggtcatcgt ggatttgggc cgtaaactat
     7981 tgtgcatcca acaaggtggc aagctatttg tatggtgatc ctcaatcaac gcaagagttg
     8041 ttttcaattc caatttacaa ttggaccgct ccttttgacg atccgggaag tttaaccaaa
     8101 tcttcgtttt tttataatgg taagtcgctg gggcatggca tggagggatt caaaaaaatg
     8161 gtagattcgg ttgcaaatgg taaggcgcat aaaattttga tcttggggtc cctttataat
     8221 agagacagcc agttcggaag cgatgaaaga ccttatgagc aattacaaaa tctcttagac
     8281 gctggtctca aaaagagtgg aagccaactt ataagtgttt ctcaattgcc gtaggacaag
     8341 tcctgctaag cgcaccaact ttccccttaa aattccgaaa aggatttgtc cgcaaaatga
     8401 tcatgcaatc tccgggacct cgtcaatgcc gaaatcagtt tcaaagaact cgcgcgacaa
     8461 accggcctgg gtgaaaaatc tctgcaccgg atgctcaacc gcaagggcaa tcccaccgcc
     8521 cgcaacttgg ggcttatcat caagagcata gccgaggatt tggacatcaa gccgcgcatc
     8581 gaaatcgccg cgtgccgttg agcgttgcga tctgcccgcg cgcaagcaaa tcagtccaag
     8641 tccccaagcg cacccatctt cacgaatttt ctttctgctc gatttgtttt tccatgatca
     8701 aaagttgcca cgcattcctg gtcgcgttgt agagaagcaa tgcgaccacc ggcacaagga
     8761 ggtaagtcgc ggccatttcg cctcggtaga tttcgatggt tgcggccaga agcgccagcc
     8821 aggaaattgt ttgaaacaaa tactcgcgcg cgaccttcaa cgccgcccgt ttttccctgg
     8881 tcatttttct cgttcgataa aacgcgcgga ccagccactg gcccctgaag gccagtagga
     8941 tgaccaaggg aatgacaaga tcgcgggacc ggaaacatgg gatcaggaac atcaatgcga
     9001 tgaacagcgc gtacagaaaa tcgccaaaac tgcgctgggc aagccgcaat aaaatacggt
     9061 tttcctgccc cgtgattctt tcccggttga gtgagagcgc cacgaacaac agcccggcca
     9121 a
//
LOCUS       DASZMM010000302         5635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_937330, whole genome shotgun sequence.
ACCESSION   DASZMM010000302 DASZMM010000000
VERSION     DASZMM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937330"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VG754_07225"
     CDS             complement(<1..360)
                     /locus_tag="VG754_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HWF19042.1"
                     /translation="MSVISLAVIFTCTRLVKGDETPNPAPRKPQPRCAVAKGQKAPAF
                     KVRSLDGKIINFPADYKGKVVMVDFWATWCEPCKEELPNVTAVYEKNHTKGFEVISVS
                     LDGPGDGPMLLQFVHDHG"
     gene            522..827
                     /locus_tag="VG754_07230"
     CDS             522..827
                     /locus_tag="VG754_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19043.1"
                     /translation="MPITTRMKKSGRKRVYDQKREGVRGELNGVAREIFTSGHREVTR
                     GPAGMRDPVLNEQTGLAQELLAQRRRRTLASVRRQNMKQFHKGPKRLTRVGLRRPQP"
     gene            1312..2175
                     /gene="mtnP"
                     /locus_tag="VG754_07235"
     CDS             1312..2175
                     /gene="mtnP"
                     /locus_tag="VG754_07235"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWF19044.1"
                     /translation="MAANRIGIIGGSGLYHIEGFTNQKWLTLKTPFGAPSDSFLTGQL
                     SGRDVVFLPRHGRGHRILPTELNHRANIHGMKQLGVQWIISVSAVGSLQAKYKPCDVV
                     LVDQFVDRTKQSQAHTFFGRGIVAHIAFADPICEELRQILLKSARALKARAHDGGAYV
                     NMEGPAFSTRAESVTNHKLGYDVVGMTNLGEAKCAREAEIAYATMAMVTDYDCWKVDE
                     AHVTVEMVIANLNKNAALAKAVVARAISQIPSEADWPCHHALANAIMTDKKFWPPKTK
                     KELKLLLAKYV"
     gene            2540..3238
                     /locus_tag="VG754_07240"
     CDS             2540..3238
                     /locus_tag="VG754_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWF19045.1"
                     /translation="MTYNIQHGAGADEKIDLLRTAQAIKHEKPDIVALEEVDNGVERT
                     ARRDLTAELAAMTEMTGYFSNNFNFQGGLYGNAVLTRFPILSATNTHYQMLRPKEQRG
                     IIQLILDVHGRKILFMATHIDYRKDNAERLMNVAEIKRIIAQYPGLPMILCGDFNDFP
                     NTPVHKAMQETFDDTWELIGHGDGFTFPSPKPNRRIDYIWLGKDKSVVPIKAWVPDTQ
                     ASDHRPFVADFELK"
     gene            complement(3239..3793)
                     /locus_tag="VG754_07245"
     CDS             complement(3239..3793)
                     /locus_tag="VG754_07245"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HWF19046.1"
                     /translation="MKTKLFILVIALQSLWVIGTVAVQEFTLRTGTGIMLETQPVDPR
                     DLIRGDYIHLRYTINSVSMSSFQPALSTNPLPGATVFVQLEKNNEFFAVKSASLKAMK
                     GDSEHPVLRGKIGNQWFAGRPAANASVNVVYGLEQFYVHEGTGNPRGRLTAEIAVRSS
                     GSAVLKQVYVDGKPYAEAMRDKDN"
     gene            complement(3798..4973)
                     /locus_tag="VG754_07250"
     CDS             complement(3798..4973)
                     /locus_tag="VG754_07250"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HWF19047.1"
                     /translation="MKISDIDRIQQAGLISPEQRSAIVAHFKLDKETNKLLAIFSIIG
                     GVLVSAGIILLISSNWQEIPHFIKLAGGLALMLGAHYGGMQLRREDRHPIVGEALHLI
                     GSAMFLANIALVGQIYHLSSRPPNTILLWLAGIGALPWLLRSKAQHILTLCVFGLWLG
                     LELNQEDSLIYFGGDARQFMFYAILGIAFAGLGMLLRRSKFPEFGPATEKFGLLVTHI
                     AAYPLAIGAYYASDKVNSGAWIIAGVVSALAIVLLLLSVRDEKLIPETQWRWVWAVAL
                     MGVLALAWVGLAFKSEFNWNETRYFGMHWIAIPALFGFCLLQAQVGLLRRSPWLVNVA
                     ITFIGVYVITAYFELFGTMQTTGLMFVISGVLLIGLAVFLERKRRALLKRMQPTPAV"
BASE COUNT         1382 a         1652 c         1425 g         1176 t
ORIGIN      
        1 cccgtggtcg tgcacaaatt gcaacagcat cgggccgtcg cccggcccat ccaggctgac
       61 gctgatcact tcaaaaccct tggtatggtt cttttcgtaa acggcggtga cattcggcaa
      121 ttcctctttg caaggttcgc accaggtcgc ccaaaaatcc accatcacga ccttgccttt
      181 gtaatcggcg ggaaagttga tgattttgcc gtccagcgag cggactttga acgccggcgc
      241 tttctgtcct ttcgcgactg cgcaacgagg ctgcggtttt ctcggcgcag gatttggagt
      301 ctcgtcgcct ttgacaaggc gcgtgcaggt aaaaattact gcgagagaaa tgaccgacaa
      361 ggttgagcac cgaatcaatc tcaatgatgt gaaagccttc atgcccgaac cataacccgg
      421 agcgtccctg ttgcaatcca aaattttccc tgcgttaatc aggatgacca actctcgaac
      481 cgcgtttata ttacttatgt taaatctaaa aaggagatgt catgccaatc acaaccagga
      541 tgaaaaagag cggacgcaaa cgcgtttatg atcaaaaacg ggaaggcgtc cgtggagaac
      601 tcaacggagt cgcgcgagaa atattcactt ccggacacag ggaagtcacc cgtggccccg
      661 cgggcatgcg cgacccggtg ttaaatgagc agaccggtct cgcgcaagaa ctgctcgcac
      721 aacggcgtcg gcgcacgctc gcctcagtgc ggcggcaaaa catgaaacaa ttccataaag
      781 gcccaaaacg cctcacgcga gtcggtctca ggcgacctca accgtgagcc gcggaagcgg
      841 cgaaagaaca caaggtatgg agcgcgatgc tgcgcgaagc gccagccgca gcaagccatc
      901 aaattctgaa tgttcaaggc tgctgcggta ctgtgggaca cagccgcgct catttctgaa
      961 tccagatgaa gaccccgggc gcatctcagt ttcttgcaca acggagtgat tgaacatttc
     1021 cttccccctc accccgccct ctcccttggg gagagggagc acgcatcccg tcgtggttgc
     1081 aaaccgagag cgttcggatt atcgagcaag ggccaaatgg tcccccctct ccttggggag
     1141 agggctgggg tgagggggac agcgatgtgt gccagcccat gaaccacaat tcgcaagagc
     1201 ccagaaacgt gcaaaatagt gagctgcgcc cgaagacccc gccttttatt ttggacattg
     1261 gacacacgcc atcgattttc ctataatgcc gccgaacgtt tgaattcaac tatggcggca
     1321 aatcgaatcg gcatcatcgg cggcagcggg ctttatcaca tcgaaggctt caccaaccaa
     1381 aaatggctca cccttaaaac gcctttcggc gcgccttccg atagtttcct gaccggccag
     1441 ttgagcgggc gcgatgtcgt tttcctgccg cggcacggac gcggccatcg catcctgccg
     1501 accgagctta atcatcgcgc gaacatccat gggatgaagc aactcggcgt gcaatggatc
     1561 atcagcgtca gcgccgtcgg ttcattgcag gccaaataca aaccctgtga cgtcgtgctg
     1621 gtggaccaat ttgttgaccg cacgaaacaa tcgcaggcgc atactttttt cggacgcggc
     1681 atcgtcgcgc acatcgcgtt cgcggacccg atctgcgaag agttgcggca aattcttttg
     1741 aaaagcgcgc gggccttgaa agcccgcgcc cacgacggcg gcgcgtatgt gaacatggaa
     1801 ggccccgcgt tcagcacccg cgccgaatcc gtcaccaatc acaaactcgg ttacgatgtc
     1861 gtgggcatga ccaatctcgg cgaggccaaa tgcgcgcgcg aagcggaaat cgcctacgcg
     1921 accatggcca tggtcaccga ttacgattgc tggaaagtgg acgaggcgca cgtgaccgtc
     1981 gagatggtca ttgccaatct caacaaaaat gccgcgctcg ccaaggccgt tgtcgcgcgc
     2041 gcgatttcgc aaattcccag cgaagccgac tggccgtgtc atcatgcatt ggccaacgcc
     2101 atcatgaccg acaagaaatt ctggccgcca aaaaccaaga aggaattgaa gctgttgctc
     2161 gcgaaatacg tctgacagtc cataaacatt tatgcgaatt acgttttttg aagtgtcagc
     2221 ggttcgttcc ccctcacccc ggccctctcc cccaggagag ggtgaaacat ttgccgccga
     2281 attggcgaat tgcgctattt ggattttcgc ctcgttagcg ggaaaacaga aagacggaga
     2341 ctggaaccgc gattctcaaa atcgccaacg tcgtggacac gctctccctc tcctggggga
     2401 gagggccggg gtgagggcga acattcctca aaaaaactcg ttgcttgccg gttcgccggt
     2461 attgttattc attattttat tctccctatc gttttgcgcc aacgcccagc caaccaacgc
     2521 gcctttcacc tttcgcgtga tgacgtacaa catccaacac ggcgcgggcg ccgacgagaa
     2581 gattgacctg ctccgcaccg cgcaggccat caaacacgaa aaacccgaca tcgtcgcgtt
     2641 ggaggaagtg gacaacggcg tggaacgcac cgcgcgacgc gatttgaccg ccgaactcgc
     2701 cgcgatgacc gaaatgaccg gttatttcag caacaacttc aacttccaag gcggcctgta
     2761 tggcaacgcg gtgctcacgc gctttcccat tctttccgcg accaacacgc attaccaaat
     2821 gctccgcccc aaggaacaac gcggcatcat ccagctcatc ctggacgtgc atggacgcaa
     2881 aattcttttc atggccacgc acattgatta tcgcaaggac aacgccgaac gcctgatgaa
     2941 cgtcgccgaa atcaaacgca tcatcgcgca atatcccggc ctgccaatga ttctttgcgg
     3001 cgacttcaac gatttcccca acacacccgt ccacaaagcg atgcaggaaa ctttcgacga
     3061 cacctgggaa cttatcggcc acggcgacgg attcaccttc cccagtccca agcccaatcg
     3121 ccgcatcgat tacatctggc tcggcaaaga caaatccgtc gtacccatca aagcctgggt
     3181 gcctgacacg caagcatccg accatcgccc tttcgtcgcg gattttgagt tgaaataatc
     3241 aattatcctt gtcgcgcatc gcttcggcgt acggcttgcc atccacgtaa acttgcttga
     3301 gtaccgcaga gccggaagag cggacggcaa tctccgcagt caaacggccc cgaggatttc
     3361 ccgtgccttc atggacataa aattgctcca aaccgtaaac cacattcacg cttgcattgg
     3421 ctgcgggcct gcccgcgaac cattggttcc cgattttccc gcgcaaaacg ggatgctcgc
     3481 tgtcgccctt catcgccttc aacgaagcgg acttcaccgc aaaaaattca ttgttctttt
     3541 ccagttgcac aaaaaccgtt gcgcccggca gcggattggt gctgagcgcg ggctggaacg
     3601 atgacatcga caccgaatta atcgtgtagc gcaaatgtat ataatcgccg cgaatcaggt
     3661 cgcgcgggtc cacgggctgc gtttcaagca tgatgcccgt tcccgtgcgc aacgtgaatt
     3721 cctgcacggc gacggtgccg atgacccaca agctttgcag cgcgatgacc aggatgaaaa
     3781 gttttgtttt catggattca cacggcgggc gtcggctgca tccgtttcaa taatgcgcgg
     3841 cgtttccgtt ccagaaaaac cgccagtcca atcagcaaca cccccgaaat cacgaacatc
     3901 aatccggtcg tttgcatcgt gccaaacaat tcaaagtaag ccgtgatgac atacacgccg
     3961 atgaacgtga tggccacgtt caccagccag ggactgcgcc gcaataaacc aacttgcgct
     4021 tgcagcaggc aaaaaccgaa caacgcggga atggcgatcc aatgcatgcc gaaatacctc
     4081 gtctcgttcc aattgaattc ggatttgaat gccaggccga cccatgccaa tgcaagaact
     4141 cccatcaacg ccacggccca aacccagcgc cactgtgttt cagggatcag cttttcgtcc
     4201 ctgacgctga gcagcagtaa aacaatcgcc aaggccgaaa ccactccggc aatgatccac
     4261 gctccggaat tcactttgtc cgaggcgtaa tacgctccaa ttgccagcgg atacgccgcg
     4321 atgtgcgtca ccagcaagcc gaatttttcc gtcgcgggac cgaattccgg aaatttgctc
     4381 cgccgcaaaa gcattcccag tcccgcaaac gcaattccaa gaattgcata aaacatgaat
     4441 tgccgcgcgt cgccgccaaa ataaatcagg ctgtcttcct ggttcaattc caagcccagc
     4501 cagagaccga acacgcagag cgtcaggatg tgttgcgcct tcgagcgaag caaccatggc
     4561 aaggcgccga tgccagccag ccagagcaag atcgtgttgg gcgggcgcga actgagatga
     4621 taaatctgtc ccaccagcgc gatgttcgcc aggaacatag cggagccgat caagtgcaac
     4681 gcctcgccca caattggatg gcggtcttcg cggcgcaatt gcatcccgcc ataatgcgcg
     4741 cccaacataa gcgcgagtcc gccagccagc ttgatgaaat gcggaatctc ctgccagttc
     4801 gacgaaatca agagaatgat tcctgcgctg acgaggacgc cgccgatgat cgagaagatg
     4861 gccagcaatt tgttcgtttc cttgtccaac ttgaaatgcg cgacaatggc cgaccgctgc
     4921 tcaggggaaa tcaacccggc ctgctggatg cgatcaatgt cggaaatctt catggcgcga
     4981 gaaagtgaac tgatcttggg cttgggttca tctcattgaa aatcataggc aaatgaactc
     5041 ttaagtcgag ggcattcgga acgataaaat ttcccaaaat cggctgtcaa tatgatggat
     5101 gagctgtttc attgtcactt cctgtttaac accccttgca tcggaactgt taattgaaaa
     5161 acctcggcca atcatcattt tattcgatga acacgtcggc caataagttc acccattttg
     5221 cagcctggag cgtttggaaa ttatgacacg aattcctctc gtgaacaccg ggcttcagtc
     5281 cgatgagaaa gcctctgcgc ggcgtcaacc ccttttcaag ggttcatctg cgtccatccg
     5341 tccatccgtg gttaaaaggc cttttcccat gcagaaaatc tgaaaagaac gacacgaatt
     5401 acactaattt tcacaaattg ggaatcattt tgggtgaatt cgtgttaatt cgtgccaatt
     5461 cgtgtctctc cttctccgtt taaatccgtg tgtatccatc tgtggctttg aatagggaac
     5521 ggagcgcggg tcggtgaccc gcagcaatgt gggaaggatg ggcgcacatg aattagcccg
     5581 gcatatcctg tttgtcgcgc gttgctgcgg ctcatcgagc cgcgctcccc tgatt
//
LOCUS       DASZMM010000303         9441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3141092, whole genome shotgun sequence.
ACCESSION   DASZMM010000303 DASZMM010000000
VERSION     DASZMM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141092"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1536)
                     /locus_tag="VG754_07255"
     CDS             complement(229..1536)
                     /locus_tag="VG754_07255"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF19048.1"
                     /translation="MKANILVLDDDTEIRELLAQVLQRSNFSVFQVKDGAGLKEAYGR
                     FQPDVVLLDYKLPDATGLDLLPLIKKEWPEAEVIMLTGHATYDVAVEATKRGAFHFQE
                     KPFDPESLLNLIKRALEIRTLREAVSTGNAIFQCEVMKSVVRTVRRVATSDVSILITG
                     ESGTGKEVIADLIHSSSARSSGPLIKINCAALPRELIESELFGSVKGAFTGAHSDREG
                     LFRQAEGGTLLLDELSEMPIDTQSKLLRVLQEKEVRPVGGRTSYKTDCRIIAATNRKP
                     EEAIRDGKLREDLFYRISAVSVHLPPLRERREDILPLANAFLKRFSAQANRDIIGFSD
                     EAAERLRSFDWPGNVRQLQNEVQRAVLLCEGKAVEGVDLSITSANNEPAGDTSFTLLE
                     GVERNTIVQMLKETGGNKLETAKRLGIGRQTLYNKIKAYGIET"
     gene            complement(1533..2336)
                     /locus_tag="VG754_07260"
     CDS             complement(1533..2336)
                     /locus_tag="VG754_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWF19049.1"
                     /translation="MKDSRTKKSDFKKAPLVLISPYTVTKGEELGDLSISLSEAYQRA
                     IMIAGGLPLALPCIPSREMLAEMVRRCDGVLFTGGDDVDPKLYGKKLAPDLQKTLTVE
                     GAERDLREMILVDEVFQQRKPLLAICRGHQMVNVALGGTLVVDIKKQVPGALNHRQLE
                     KKAEVVHEARLTGDSLLAKIAGKQKLGVNSTHHQAVGRVAKPLRVTAAADDGIVEAME
                     LRREDAGLLPYLMTVQFHPERLASRHAEHQELFNSFIRACARNRSKSKL"
     gene            complement(2308..2577)
                     /locus_tag="VG754_07265"
     CDS             complement(2308..2577)
                     /locus_tag="VG754_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19050.1"
                     /translation="MAKAEISWKRRTEEGERVEVYAQHVGDQWKFFMRDRRFEQWQAV
                     AEPPLEDWLELLDAVQRRISRRLLRPEEEARVKKSIHERFPDQKI"
     gene            complement(2692..3174)
                     /gene="ispF"
                     /locus_tag="VG754_07270"
     CDS             complement(2692..3174)
                     /gene="ispF"
                     /locus_tag="VG754_07270"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWF19051.1"
                     /translation="MVHVGIGYDVHQLVTGRKLVLGGVEIPHTKGLDGHSDADVLMHA
                     ICDAVLGALGEADIGHFFPNTDPRWKGAPSRVFLEEAARQANFHQAKIVNIDATIIAQ
                     QPKVYPHVAQMKINIASALGININRVGIKATTNEHMGFIGREEGIAAMAVASVERAEG
                     "
     gene            3366..3956
                     /locus_tag="VG754_07275"
     CDS             3366..3956
                     /locus_tag="VG754_07275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWF19052.1"
                     /translation="MSEPTLKVLAVIGSLHKKSVTRSVIQHVAKKLVTGGCTVDILDF
                     EKEPLALYNPDTAHEGPAYAALRARVDNADVILLGTPDYHGSISSATKNFLDHFWREF
                     AGKLFATIVASHEKGLTATDQLRTIARQCYAWTLPYGVSFTEGADVKEGEIVSDNFKN
                     RLEMMIRDVRTYGALLSRQRASDLKGSEPGFMARLR"
     gene            complement(3965..5560)
                     /locus_tag="VG754_07280"
     CDS             complement(3965..5560)
                     /locus_tag="VG754_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF19053.1"
                     /translation="MKTEDSKDVPRKPRKMRWWMPSVILLIAVATITALNQAPELPII
                     KKIMFGMLTVVVSALLLLVWFMFLSAFRWRTRFIGLAVIVLLVFGFSRVVRIDGAIDG
                     SGQPRLVWKWTPKNTGLVKAQPVAMLSTSTTARKPEVNVAFPGLLGNSRNGVISGILL
                     DRDWTAHPPQQVWRQPIGLGWAGFAIAGSTAVTLEQRAENELVVAYELTTGNTLWMHT
                     NRVRFSEKMGGDGPRSTPTIANGRVYTVGATGILNCLDLQDGRLIWSHDVLGETHQPN
                     LIFGVSCSPLIVDDLVIVTGGLTNGPTLLAYRIDNGSNIWKSGADNASYSSPMLATLC
                     GKRQVLSVNEASVTAHNPADGRILWKYDWKKWGPRCAQPVIVEGDRVFLSAGFGVGCV
                     LLQIKSDAAGQLSAEELWKNLRLKTQFSTAVARGNFVYGLDEGVLACINLATGERAWK
                     DGHYGYGEVLMIDDMLLVQTEPGAVTLVEADPAQYREVARFGALNSKTWNNPALAGEY
                     FITRNDQEAICYRLPLQKPRSIN"
     gene            5653..6162
                     /locus_tag="VG754_07285"
     CDS             5653..6162
                     /locus_tag="VG754_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWF19054.1"
                     /translation="MSRTPLKKNDEHCSPGVAFLLSQIGAHAAGKFAARLAPLKLAPS
                     HAGILRVISADAGISQQKLAKFLGMFPSRLVLVLDEMEKAGLVERKASVADRRTYALQ
                     LAPKGREALQAIGRIAREHQEELCAAISPKERETLAALLSRIARHQHLTPGVHPGYKQ
                     IGRGCGRPC"
     gene            6214..7254
                     /gene="nadA"
                     /locus_tag="VG754_07290"
     CDS             6214..7254
                     /gene="nadA"
                     /locus_tag="VG754_07290"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWF19055.1"
                     /translation="MIDVTDIPLRGVDNLQVRKPLPRAQFEPLAAEILALKKKLNAII
                     LAHNYQVPEIQDVADFVGDSLGLSQQAAKTDADVIVFSGVHFMAETAKILNPEKIVVL
                     PDMKAGCSLEESCPAPKLAKLQATNPNFYTVTYINCSAAVKALSDVICTSGNAVKIVE
                     AAPKDRDLLFVPDENLGAWVMEQTGRPMTLWKGNCYAHVEFRRDAVLQLRKLHPDAKI
                     VVHPESLREVRDLADAVCSTEKMIDYCKKSTAKRFVIVTESGIIHRMQKECPDKEFLC
                     APVFDAMRAPTDNCRCSECRYMKMNTLEKLRDCMKNLAPRIELPAEIIRKARLPIERM
                     LEISARPLENAG"
     gene            complement(7268..7552)
                     /locus_tag="VG754_07295"
     CDS             complement(7268..7552)
                     /locus_tag="VG754_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HWF19056.1"
                     /translation="MRELVLTSRFERSFRRLVRKAPSLQQHIETTLSLLSVDLSNPKL
                     KTHHLSGPLSGYFACSCGYDCRIVFAKEKSKEQGKEVLILIDIGSHDEVY"
     gene            complement(7555..7788)
                     /locus_tag="VG754_07300"
     CDS             complement(7555..7788)
                     /locus_tag="VG754_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19057.1"
                     /translation="MAALTLDQVLTYAESLPAEEQEILADLLKKRRIAAWREETGTYA
                     RETVTAYQVGKLKAQPANSVISRLRSGLKKSSG"
     gene            complement(7855..8322)
                     /locus_tag="VG754_07305"
     CDS             complement(7855..8322)
                     /locus_tag="VG754_07305"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWF19058.1"
                     /translation="MKSAFMREAIRISVQMMRRGKGGPFGAVVVRKGKIVGKGWNRVT
                     SANDPTAHAEVVAIRDACRRLKTFQLADCELYTSCEPCPMCLSAIYWARLKKIYYANT
                     RKDAAGIDFDDDFIYTEVSRPISERKLVMKQLMRKEALAAFEEWKRKTDKVRY"
     gene            complement(8319..9176)
                     /locus_tag="VG754_07310"
     CDS             complement(8319..9176)
                     /locus_tag="VG754_07310"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWF19059.1"
                     /translation="MNPYQHLVSEYARFAKDGKSYPLGKFPRLPRPDLPANAPKVLIF
                     SPHPDDEVIIGGLALRLLREANWNIINVAVTQGSKKERQAERLAELKSCCDCIGFGLV
                     QTAPSGLEKINLKTREQDAAHWAASVKVIADILMKHQPKVILVPHDNDWNSAHIGTHF
                     LVMDALKTLSANFSCHVVETEFWGAMASPNLMVEISPRDLGDLITALTFHVGEVQRNP
                     YHLTLPAWMVDNVRRGGELVGGQGGAAPDFVFATLYRLRKWSGGKLEKIFDGGRSLGA
                     NENPMALFK"
     gene            complement(9212..9322)
                     /locus_tag="VG754_07315"
                     /pseudo
     CDS             complement(9212..9322)
                     /locus_tag="VG754_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT         2130 a         2831 c         2372 g         2108 t
ORIGIN      
        1 aatccaaaca gttttgtggc gtggtcaacc aattcctccg ttcgctacac ctttacttat
       61 gacccacacg ccgcagatgt gcggctgttt gaattcgcgg gaggaacggt ccgcgccaca
      121 aattctgagt ctttgccgaa gccaaaatgg acttggacga attagagaca aaagcgccta
      181 aaactcagcg ctgtctgaaa cgaagtacaa gtttcgtccg ggcaagattt acgtctcgat
      241 cccgtacgcc ttgatcttgt tgtaaagcgt ttgccgtccg atgcctaggc gcttggcggt
      301 ttccagtttg ttgccaccgg tttccttcaa catctggaca atggtattgc gctcgacgcc
      361 ctccagcaac gtaaagctcg tgtcgcccgc cggttcgttg ttcgccgagg tgatggaaag
      421 atcaacgccc tcaaccgcct tgccttcgca caaaagcacc gcgcgctgca cttcgttctg
      481 caattggcgc acattgccgg gccaatcgaa cgaacgcaat cgttccgccg cctcgtccga
      541 aaaaccgatg atgtcccggt tcgcctgcgc ggaaaaacgt ttcaaaaacg cgttcgccag
      601 cggcaaaata tcttcgcgcc tctcgcgcaa gggcggcaga tgcaccgaca ccgcgctgat
      661 acgatagaac aaatcctcgc gcaatttgcc atcgcggatg gcttcctccg gcttgcggtt
      721 cgtcgccgcg atgatgcggc aatcggtctt gtaactcgtt cgcccgccga ccggccgcac
      781 ttctttttcc tgcagcacgc gcagcaattt gctctgcgtg tcaatcggca tctcggacaa
      841 ttcatccagc aacagcgtgc cgccttccgc ttggcggaaa agaccctcgc gatcgctgtg
      901 cgcgccggtg aacgcgccct ttaccgagcc gaacaattcg ctctcgatga gttcgcgggg
      961 cagcgcggcg caattgattt taatgagcgg gccggaagat cgcgcgctcg aagaatggat
     1021 caaatccgcg atgacttcct tcccggtgcc gctttcgccg gtgataagaa tggaaacatc
     1081 gctcgtggcg acgcggcgga cggtgcgcac gaccgacttc atcacctcgc attggaagat
     1141 cgcattgccg gtcgaaaccg cctcccgcaa ggtgcggatt tccagcgcgc gcttgataag
     1201 attcagcagg ctttccgggt cgaaaggctt ttcctggaaa tgaaatgcgc cgcgtttggt
     1261 cgcttccacg gcgacgtcat aagtcgcatg gccggtgagc atgatgactt ccgcctcggg
     1321 ccattccttt ttgatgagcg gcagcaaatc aaggccggtg gcgtccggca gcttgtaatc
     1381 cagcagtacc acgtccggct gaaagcgccc gtaggcttcc ttcaagcccg cgccgtcctt
     1441 cacctggaag accgagaaat tgctgcgctg caaaacctgc gccagcaatt cgcggatctc
     1501 cgtatcatca tccaagacca ggatatttgc cttcataatt tgcttttgct gcgattccgg
     1561 gcacaggccc ggataaaact gttgaacaat tcctgatgct ccgcatgccg ggacgccagg
     1621 cgttcaggat gaaattgcac cgtcatcaaa tagggcaaca atccggcgtc ttcccgccgc
     1681 aattccatcg cttccacaat gccatcatca gcggccgcgg tcacccgcaa cggctttgcc
     1741 acgcggccta cggcctggtg gtgcgtgctg ttgacgccca acttttgctt gccggcaatc
     1801 ttggcaagca atgaatcccc tgtcaaccgt gcttcatgca caacttcggc ctttttctcc
     1861 agttgccggt ggttcaacgc acccgggacc tgcttcttga tatcgaccac caaagtcccg
     1921 cccaaggcca cattcaccat ctgatgtccc cggcaaatcg ccagcaatgg cttgcgctgc
     1981 tggaaaactt cgtccaccag gatcatctcg cgcaaatccc gctctgcgcc ctccaccgtc
     2041 aacgttttct gcaaatcggg cgcaagcttt ttcccataaa gctttgggtc aacgtcatct
     2101 ccaccggtga aaagcacgcc gtcgcaacgc cgcaccatct cggccagcat ttcccgcgac
     2161 ggaatgcacg gcaacgccag cggcaatccg ccggcaatca tgatcgcccg ttgatacgcc
     2221 tccgaaaggc tgatggacag gtcgccgagt tcctcgccct tggtgacggt gtaaggtgaa
     2281 atcaaaacca gcggcgcctt cttgaaatca gattttttgg tccgggaatc tttcatggat
     2341 tgatttcttg acgcgcgctt cttcttccgg ccgcaatagg cggcggctga tgcggcgctg
     2401 cacggcgtcc agcaattcca gccagtcttc caatggcggt tcagccacag cctgccactg
     2461 ttcaaaacgc cggtcacgca taaaaaattt ccattggtcg ccgacgtgtt gcgcgtaaac
     2521 ctcgacacgt tccccttcct ccgtccgccg tttccaacta atttcagcct ttgccataat
     2581 tccaatcaaa acatcgaacg ctgaacatcg aacttccaac ttcgaattga aaagaggggt
     2641 ttgcccacgc gttccattgg atgttcagcg ttcagtgttg gatgttcgat gttatccttc
     2701 cgccctctcc acgctcgcga ccgccattgc cgcaattcct tcttcgcgcc caataaagcc
     2761 catgtgctca ttcgtcgtcg ctttgatgcc aacgcgattg atattgatgc ccaatgccga
     2821 agcgatgttg atcttcatct gggcgacatg cggataaacc ttcggttgct gggcgatgat
     2881 ggtagcgtcg atgttcacaa tttttgcctg atgaaaattc gcctgacgcg ccgcttcctc
     2941 caaaaacacc cggctcggcg cccctttcca gcgcgggtcc gtgttcggaa aaaaatggcc
     3001 gatgtccgcc tcgcccaacg cgcccaaaac ggcgtcgcaa atcgcgtgca tcaaaacgtc
     3061 cgcatccgaa tgaccgtcca aacccttcgt gtgcggaatc tccaccccgc ccaaaaccag
     3121 cttgcggccc gtcaccaatt ggtgaacgtc gtaacctatg ccaacatgaa ccattcggcg
     3181 aatttaacca ccaagacaca aagacaccaa gaaattaaag cgcgcgcgaa gaccgcaaag
     3241 gtgaaaatga tttaatatct ggtggtgcga gcgtactcgc gagccttccc atcgctcaaa
     3301 ccctccgtca cctgcctttc accactccgt tgccttttct tattttgtgc gctaatttgc
     3361 gccccatgtc agaaccaacc cttaaagtcc tggccgtcat cggcagcctg cacaaaaaat
     3421 cagtcacacg cagcgtcatc cagcacgtcg caaaaaaact tgtgaccggc ggctgcacgg
     3481 tggacatcct cgatttcgaa aaagaaccgc tcgcgcttta caatcctgat accgcgcatg
     3541 aaggtcccgc ttacgccgcc ttgcgcgccc gcgtggacaa cgccgacgtc atcctgcttg
     3601 gcacgcccga ttatcatggc agcatcagca gcgcgaccaa aaatttcctc gaccatttct
     3661 ggcgcgaatt tgccggcaaa cttttcgcca ccattgtcgc ttcgcacgaa aaaggactca
     3721 ccgcgaccga ccagctccgc accatcgccc gtcaatgcta cgcatggact ttgccctacg
     3781 gcgtctcctt caccgaaggc gcggacgtca aggaaggcga aatcgtgagc gacaatttca
     3841 agaaccgtct ggaaatgatg atccgcgacg tgcgcactta cggcgctttg ctcagccgcc
     3901 aacgcgcatc cgatttgaaa ggcagcgaac cgggtttcat ggcgaggttg cgataagccg
     3961 gccgttagtt gatggaacgc ggcttttgta aaggcaaccg gtaacaaatc gcttcctggt
     4021 cgttcctggt gatgaaatat tcaccagcca gcgccgggtt attccaggtt tttgaattca
     4081 atgcgccgaa ccgcgccact tcccgatatt gcgccgggtc cgcctccacc aacgtcaccg
     4141 cgccgggttc tgtttgcacc aggagcatgt cgtcaatcat cagaacctca ccgtaaccgt
     4201 aatgcccgtc cttccacgcg cgctctcctg tggcaagatt gatgcaagcc aggacgccct
     4261 catccaatcc gtaaacgaaa ttgccccgcg ccacagccgt tgaaaattgt gttttcaatc
     4321 tcaagttttt ccacagttcc tccgcagaaa gttgcccggc ggcatccgac ttgatttgca
     4381 gcagcacgca accgacacca aatcccgcgg acagaaacac ccggtctccc tccacgatga
     4441 cgggttgcgc gcagcgagga ccccattttt tccaatcgta tttccagaga atccggccat
     4501 cagcgggatt gtgcgccgtg acactggctt cgttcacgga caatacctgc cgtttgccgc
     4561 agagtgtcgc gagcatcggg gaactgtaac tcgcattgtc cgcaccgctt ttccaaatgt
     4621 tcgaaccatt atcaatccga taggccaaaa gtgtgggacc attggtcagt ccgccggtga
     4681 cgatcaccaa gtcatccaca atcagcggcg aacaactgac gccaaaaatg agattcggct
     4741 gatgtgtttc tcccaggaca tcatgcgacc aaatcaaccg gccatcctgc aaatcaaggc
     4801 aattcaaaat cccggtcgcg cccacggtgt acacccgacc attggcgatt gtaggtgtcg
     4861 agcgaggacc atccccgccc attttttcgc tgaatcggac gcggttggta tgcatccata
     4921 acgtgttgcc agtcgtcaat tcataagcga ccaccagttc attttccgcc cgttgctcca
     4981 aggttaccgc tgtcgaaccg gcaatggcaa atcccgccca tcccaaaccg atcggctgac
     5041 gccaaacctg ttgcggcgga tgcgccgtcc aatcccggtc cagcaaaatt ccactgatca
     5101 ccccgttccg gctgttgccc agcagaccgg gaaacgcgac atttacctcc ggtttcctcg
     5161 ccgttgtgct tgtgctcaac attgccaccg gctgcgcctt gaccaaaccg gtgtttttcg
     5221 gggtccattt ccacaccagt ctaggctgac ccgagccgtc aatcgctccg tcaatccgaa
     5281 ccacgcgaga aaaaccaaac accaacaaca caatgaccgc cagcccaata aaacgcgtgc
     5341 gccacctgaa tgcggaaaga aacatgaacc ataccagcag cagcaacgcc gagaccacca
     5401 cggtgagcat gccgaacatg attttcttga tgatcggcaa ttccggcgcc tggtttagcg
     5461 ccgttatcgt tgcgaccgcg atgagcaaaa tcaccgatgg catccaccag cgcatcttgc
     5521 gcggtttgcg gggaacgtcc ttcgaatctt cagttttcat aacgagttgc gttgaataca
     5581 gggattgaaa agctgtgcga atccgtttat actcttaatc gttaatcgag ttaataattc
     5641 atttgattta acatgtcaag aacgcctttg aaaaaaaatg acgaacattg cagccctgga
     5701 gttgccttcc tgctttcgca gattggggcg catgcggcgg ggaagtttgc cgcgcgattg
     5761 gcgccgctca aactcgcgcc ctcccatgcc ggcattctgc gcgtcatcag cgccgatgct
     5821 ggcatcagcc agcaaaagct cgcgaagttt ctaggcatgt ttcccagccg tctggttttg
     5881 gtgctggacg agatggaaaa ggcgggcttg gttgaacgaa aggcgagcgt tgcggaccgt
     5941 cgcacctacg cattgcaact agcgcccaag ggaagagagg cgttgcaggc gattggccgc
     6001 atcgcacggg aacatcagga agaattgtgc gcggccatca gcccaaaaga acgcgaaacg
     6061 cttgcggcgc tcctgtcgcg aatcgcccgg catcagcatc tcacccccgg cgtccatccc
     6121 ggctacaaac aaattggccg cggttgcggg aggccgtgct gaccgttcca ttgcaagctt
     6181 gccgcgtttt accatcaccc ttagatttat ctcgtgattg atgtgactga tattccgttg
     6241 cgcggtgtgg acaatttgca agtccgcaag ccgctgccgc gcgcgcagtt cgagccgctg
     6301 gccgcggaaa tcctcgcgct caagaaaaag ctcaacgcaa tcatcctcgc gcataattat
     6361 caggtgccgg aaatccagga cgtggccgat tttgtcggcg attcgttggg gctttcgcaa
     6421 caggctgcca agacggacgc ggacgtgatt gttttctccg gcgtccattt catggccgag
     6481 acggcgaaga ttttgaaccc ggaaaaaatc gtcgtgctgc cggacatgaa ggctggttgt
     6541 tcgctcgagg aaagttgtcc cgcgccgaaa ctggcgaagt tgcaagcgac caatccgaat
     6601 ttttacacgg tcacttacat caattgcagc gcggcagtga aggcgctcag cgacgtgatt
     6661 tgcaccagcg gcaacgccgt caagatcgtc gaggccgcgc cgaaagaccg cgatttgctt
     6721 tttgtgccgg atgaaaatct cggcgcgtgg gtcatggaac aaaccggccg tccgatgacg
     6781 ttgtggaagg ggaattgtta cgcgcacgtc gagttccgcc gcgacgcggt gctgcaattg
     6841 cgtaagcttc atcccgacgc gaaaatcgtc gtgcatcccg agagcttgcg cgaggtgcgc
     6901 gatttggccg acgcggtttg ctcgacggaa aagatgattg attattgcaa gaagagcacg
     6961 gcgaagcgtt ttgtgatcgt taccgaatcg ggcatcatcc atcggatgca gaaggaatgt
     7021 ccggacaagg aatttttgtg cgcgccggtt ttcgacgcga tgcgcgcgcc gacggacaat
     7081 tgccgttgca gcgaatgccg ctacatgaag atgaacacgc tggaaaaatt gcgcgactgc
     7141 atgaagaatc tcgcgccgcg catcgaactg cccgccgaaa tcatccgcaa agcgcgtttg
     7201 ccgatagagc ggatgctgga aatttcagcc aggccgctgg aaaatgcggg gtgaggcgat
     7261 tcgattttca gtaaacttcg tcgtgagaac caatatcaat cagaattagg acctccttgc
     7321 cctgctcctt gcttttctcc ttcgcaaaca caatccggca atcgtatccg catgaacatg
     7381 cgaaataacc tgacaatggc ccgctcaagt gatgcgtttt cagtttggga ttgctcaaat
     7441 caacgctcaa taacgaaagc gtcgtttcaa tatgctgttg caaagaaggt gcctttcgaa
     7501 ccagacgacg gaatgacctt tcaaagcgcg aggttaatac cagttcgcgc attgttatcc
     7561 agaagacttt ttcaatccag aacgcaaacg ggaaatgact gaattcgcag gttgtgcctt
     7621 gagcttgcca acttggtagg cagttactgt ttcgcgcgca tacgtgccgg tttcttcccg
     7681 ccatgccgct atgcggcgtt tcttgagcaa gtcggccaaa atctcctgct cctccgctgg
     7741 cagactttca gcgtatgtca gaacctgatc gagagttaaa gcggccatat atttggcttt
     7801 cttacgaatc aaaaatgtcg cacacaaacc tgaatgtcaa tccatcatcc gttctcaata
     7861 ccgcaccttg tccgtctttc gcttccattc ctcaaacgct gccaaagctt ctttccgcat
     7921 aagttgtttc atcaccagct tgcgctcgga gatgggacgc gacacttcag tgtagataaa
     7981 gtcgtcatcg aaatcaattc cggcggcgtc cttgcgggtg ttggcgtaat agattttttt
     8041 taaccgcgcc cagtaaatcg ccgagagaca catcggacac ggttcgcaac tggtgtatag
     8101 ttcgcaatcg gcgagctgaa aagttttcaa gcgcctgcaa gcgtcacgga tggcgaccac
     8161 ttccgcgtgt gcagttggat cattcgccga ggtgacccgg ttccagcctt tgccaacgat
     8221 tttgcccttg cgcaccacca ccgcgccgaa cggcccgcct ttgccgcgcc gcatcatctg
     8281 gacggagatg cggatggctt cgcgcatgaa ggcgcttttc atttgaagag cgccatggga
     8341 ttttcgttcg cgcccagcga acgtccaccg tcaaaaattt tttccaactt gccgccgctc
     8401 catttgcgca gccgatacaa cgtggcaaaa acaaaatccg gcgcagcgcc gccctgtccg
     8461 ccgaccaatt cgccgccacg ccgcacattg tccaccatcc aggcggggag cgtaagatga
     8521 tacggattgc gctgcacttc cccgacatga aacgtcaatg cagtgatgag atcgccaagg
     8581 tcgcgcggac tgatttccac catcaagttc ggcgatgcca tcgcgcccca aaattccgtt
     8641 tcgacgacat gacacgaaaa attcgcggac agagtcttca acgcgtccat caccagaaaa
     8701 tgcgtgccga tatgcgcact gttccaatca ttgtcgtgcg gaacgagaat caccttcggc
     8761 tgatgcttca tcaaaatatc cgcgatgact ttcaccgaag ccgcccaatg cgccgcgtcc
     8821 tgttcgcgcg tcttgaggtt gattttctcc aaaccgcttg gcgcggtttg caccagccca
     8881 aaaccgatgc aatcgcaaca cgacttcaac tcagccaacc gctcggcttg acgctccttt
     8941 ttgctgcctt gcgtcacggc cacgttgatg atgttccaat tcgcctcgcg caacaaccgc
     9001 agcgccaatc cgccgatgat gacttcatca tcgggatgcg gcgaaaaaat cagcaccttg
     9061 ggcgcgtttg cgggcaaatc gggtctgggc aatcttggaa atttgcccag cggatacgat
     9121 ttcccgtcct ttgcaaaccg cgcgtattcc gaaacgagat gttggtatgg gttcatgctc
     9181 ctggatgaat gtcactgtaa taataagaat gttagaagga aggattatct ctggcaccat
     9241 gccagacggc aagaatttcg actagttttt gagcttcgct cacccgataa aaaatccgat
     9301 agtttcggaa gacgatttcc cggtttcgac ttctcgcgcc tctcggatag gcagggtcct
     9361 atgtatggaa aagtttctaa tacctccaca tgccggaaca agtcatcgcc aaacttctgt
     9421 gcagcgtcac ggttatcgcg g
//
LOCUS       DASZMM010000304         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_938425, whole genome shotgun sequence.
ACCESSION   DASZMM010000304 DASZMM010000000
VERSION     DASZMM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938425"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VG754_07320"
     CDS             <1..1182
                     /locus_tag="VG754_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HWF19060.1"
                     /translation="GTATRCITTEFSANGGAYKVPTFVNEFWTSKQRAASRLHEISYR
                     ACFKPQLPRFFIERLTHLGEIVYDPFMGRGTTPVEAALLGRIPYGCDINPLSSVLTQP
                     RLHPPTAEQVAERLEQIDLRDADDCPEELLVFYHPETLREIAALKKYLLERRNTRALD
                     AVDDWICLLALNRLTGHSNGFFSVYTLPPNQAVSIKSQQKINANRKQTPPRRAVSHLI
                     LKKTRNLLSDCTPEIRKTLGSVASNSLLVTKLSDSTPEIADNSVALVVTSPPFLDVVD
                     YAGDNWLRCWFLGIDPKSVKLTVPKKLDNWQEEMTAVFRELHRVLKPGGHVAFEVGEV
                     RGGKIKLEEAALPCGVAAGLQPELVLINDQIFTKTANCWGVDNNFKGTNTNRIVLLRK
                     V"
     gene            1424..1768
                     /locus_tag="VG754_07325"
     CDS             1424..1768
                     /locus_tag="VG754_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19061.1"
                     /translation="MKTIKGFQVRTMLIPCLAEFLLLANWGIAVGLRIAAAMGKGHRY
                     LPLFWDLDVVFCPVLCATIAGLIVFRHFRHRSTFVRAASLACTLGFGALFGLYILFGL
                     IALLAFPANFMQ"
     gene            1779..2147
                     /locus_tag="VG754_07330"
     CDS             1779..2147
                     /locus_tag="VG754_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19062.1"
                     /translation="MTKVSILPEPSKKGDMMYRAIAKGHQSVAKTAGAALDALTAQLP
                     ADDTVTMIVIQNQRADRFFTAQQQQRLAGLMEHWRAARDTGKGLSPAEQAELEALVDA
                     ELAASGERAASLQYLSRRDN"
     gene            2351..>2832
                     /locus_tag="VG754_07335"
     CDS             2351..>2832
                     /locus_tag="VG754_07335"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWF19063.1"
                     /translation="MNKKSSKAKSTGNDALRPYSSVVLDGPERAPSRAMLYPVGFKPE
                     DFKKPLVGIASTWSMVTPCNMHIDKLALEAEKGANEAGGKAIIFNTISVSDGISMGSE
                     GMKYSLVSREVIADSIETVVGCEGMDGFVAIGGCDKNMPGSMIAIARLNRPAVFVYGG
                     T"
BASE COUNT          624 a          760 c          778 g          670 t
ORIGIN      
        1 gggactgcga cccgctgcat caccacggaa ttttcggcaa atggcggcgc atacaaagtg
       61 ccgacgttcg tcaacgaatt ctggacttcc aagcaacgcg ccgcgagcag gttgcatgag
      121 atttcctatc gcgcctgttt caagccacag ttgccgcgct ttttcatcga gcgactgacg
      181 caccttggag agattgttta cgatccattc atgggtcgcg gcacgacgcc cgtggaagcc
      241 gcgttgctcg gccgcattcc ttacggctgc gatattaatc cgttgagcag cgttctcacg
      301 caaccgcgac tgcatccgcc cacggccgaa caagtcgccg agcggctcga acagatcgat
      361 ctccgcgacg ccgatgattg cccggaagag ttgctcgttt tttatcatcc cgaaacgctc
      421 cgcgaaatcg ccgcgctcaa aaaatatctg ctcgaacgcc gcaacacccg cgcgctagat
      481 gccgtggatg actggatttg cctgctcgcg ctcaaccggc tcaccggcca ttccaacggc
      541 tttttttctg tctatacctt gccacccaac caagccgtgt ctatcaagtc gcagcaaaaa
      601 atcaatgcga accgaaagca aacgccgcct cgccgcgcgg tgtcgcactt gattctcaag
      661 aaaacccgca atcttctcag tgattgcacg cccgaaattc gaaaaacact gggcagcgtc
      721 gcatcgaatt cattgcttgt cacaaagctg tccgattcca cgcccgagat tgccgataat
      781 tcggtcgcgc tggtggtcac gtcgccgccg tttctcgatg tggtggatta cgcgggcgac
      841 aactggctgc gctgctggtt tctcggcatt gacccgaaaa gcgtcaagct gaccgtcccc
      901 aaaaaattgg acaactggca ggaagagatg accgcagttt tccgcgaatt gcatcgcgtc
      961 ttgaaaccgg gcggacatgt ggcgtttgaa gtcggcgaag tgcgcggcgg aaaaatcaaa
     1021 cttgaagaag ccgccctgcc ctgcggcgtc gccgccggct tgcaaccgga actggtcttg
     1081 atcaacgacc aaatattcac caagacagcc aattgctggg gcgtggataa taattttaag
     1141 gggacgaata cgaatcgaat cgttttgctt cggaaggtgt aataaaagaa tgcttagccg
     1201 gaaccatgca attgagccac ggatgaaaca cggacgaaac acggattcaa aaggattgaa
     1261 taaagtttcg cggactggaa accagtcatc aaacggtgtt tcggtccaaa aggcgaaaga
     1321 ttccatcctg ctgccttttt tcggtgttcc atcggtgttt catccatggc tgaattgaat
     1381 ctttccggct tagggtactt actctgctta cttttaagtg aagatgaaga cgattaaggg
     1441 atttcaggtt aggacaatgc tcattccctg tttggccgag tttttattac tggccaattg
     1501 gggcattgct gttggtctga gaatcgctgc tgccatgggg aaaggccaca ggtatctgcc
     1561 cctgttttgg gacttggatg tggtattttg cccggtttta tgtgcgacga ttgctggatt
     1621 gattgtcttt cgccactttc gtcaccgttc aacattcgtt cgcgcagcga gtttggcatg
     1681 caccttggga tttggcgcgt tatttgggct ctatatttta tttgggctaa tcgctttgct
     1741 tgcatttcca gcgaatttta tgcaatagtt atctagtcat gacaaaagtg tccattctgc
     1801 cggagccttc aaaaaagggc gatatgatgt atcgcgcgat tgcaaagggg catcaatccg
     1861 tcgccaaaac ggccggggcc gctttggatg cgttgaccgc tcaattgcca gcggatgaca
     1921 cggttacgat gattgtcatc caaaaccagc gggcggacag gtttttcacc gcgcagcaac
     1981 agcaacgcct ggccggtttg atggaacatt ggcgcgccgc ccgcgacacc ggtaaaggtc
     2041 tctctcccgc cgaacaggcc gaactcgaag ccttggtgga cgctgaactg gccgcttcgg
     2101 gtgaacgggc cgcctcgctg caatacctgt cccgcaggga caactgagac ccggcgttca
     2161 actccgagtt ttgcggcgtt tctcacacgt gccgaaggca ctctttttcg cctgatctta
     2221 aattgcgcgt gaatgttaaa aacgtctttg gcgcgccggc ggtttcttcc gtgttcaatc
     2281 cgtgtttcat ctgtggctct attccaaaga cttgtcagcc ggggcgtttc atggtgaaac
     2341 ttgttgactc atgaataaaa aatcgtcgaa agcgaagtcc accggcaatg acgcgttgcg
     2401 tccttattcg agcgttgtgc tcgacggccc cgaacgcgcc cccagccgcg cgatgcttta
     2461 tccggtcggg ttcaagccgg aagatttcaa gaaaccgctc gtcggcatcg cttcgacctg
     2521 gagcatggtg acgccttgca acatgcatat tgacaagctg gcgctcgaag ccgagaaagg
     2581 cgcgaatgaa gcgggcggca aggccatcat cttcaatacc atcagcgtgt cggacggcat
     2641 ctcgatgggc tcggagggga tgaaatattc gctggtgtcg cgcgaggtga tcgcggattc
     2701 aatcgagacg gtggtgggtt gcgaagggat ggacggcttt gtggccatcg gcggctgcga
     2761 caaaaacatg ccgggctcga tgatcgccat cgcgcggttg aaccgcccgg cggtgttcgt
     2821 ttatggcggg ac
//
LOCUS       DASZMM010000305         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2323721, whole genome shotgun sequence.
ACCESSION   DASZMM010000305 DASZMM010000000
VERSION     DASZMM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323721"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VG754_07340"
     CDS             complement(<1..645)
                     /locus_tag="VG754_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HWF19064.1"
                     /translation="MPPLVKSFGERIADALVEDGLLTAKQVDELLEQQKKEGTRLLKL
                     VVEKSYVTEQDMTVCMGRVLNTAPVNLARIGIVPEVCELLPRDVAHNYKVVPVSRLDN
                     KLFLAMADPLNVLAIDDVKRITRLDIVPMIAAERAILDKLASIDAAKSGSMEDIINDA
                     QKQAADDDADADKLEVARESHEDVNLDQLAASSEEAPVIKLANLILVQAIKDRAS"
     gene            complement(653..1504)
                     /locus_tag="VG754_07345"
     CDS             complement(653..1504)
                     /locus_tag="VG754_07345"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19065.1"
                     /translation="MSATLTTSEESQLSQTIEMFEVITQSQPQDYQSLEILKEAYSKL
                     GREKEVIGTSKRIAQAYVQLGQLSSAILEYETILQRFPDDLDVQTALKEIETKANNFA
                     APPGISDLSSSTAKPVNGHARGSKADHGPQAEVDDGRATMRKVFVDSKLISAGDFDLC
                     WTTTDLSHPPTGVNTPFIQLLADKGIILPERSLKLLVEKSRFGYLPLEKYDVDLDLAR
                     TFPADACRRWCVMPFDRMSKTIFVATANPFNRQAAKEIADATKSRLLWYLTTPSELVT
                     NIRKAFR"
     gene            1795..>3109
                     /locus_tag="VG754_07350"
     CDS             1795..>3109
                     /locus_tag="VG754_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWF19066.1"
                     /translation="MQKLSPIMTPAPGERLRRFVGDHVRFTLSGGASALASQGWRACL
                     RTNLGRAEMLCNEILQAHTKKLPSAGASWHDLPMREENGEWHLELPCTEPGYFKSKAY
                     LIDPQGWQHWPDGPDFGISVHPDSYRTANTIYCAFTRMFGPTKNALRTADEKLEQQLR
                     QLDEKGYTVIPPSGTFRDLIQQLPHIIDRLGCRILHLLPVNPTPTVSARFGRFGSPYA
                     SLDLTGIDPALVVFDRRTTGVDQFRELTYAMHLKGGRVFLDMVINHTGWGSNLQENHP
                     EWFLRDAKGTFVSPGAWGTTWEDLVELEHKHVELWDYLAEVFLTWCERGVDGFRCDAG
                     YKVPLAAWQYIIARVRREFPETIFLLEGLGGSWEVTELLLTEGGMQWAYSELFQNYSG
                     QQVSSYLDYALKQSQRAGLFVHYSETHDNERLARLGRKWSLLRNRL"
BASE COUNT          619 a          799 c          966 g          725 t
ORIGIN      
        1 actggcgcgg tctttgatgg cctggacgag aatgaggttg gcgagcttga tgacgggcgc
       61 ttcttcgctg gaggcggcaa gctggtcaag gttgacatct tcgtgcgatt ctcttgccac
      121 ttcgagcttg tcggcatcgg catcatcatc cgccgcctgt ttctgggcgt cattgatgat
      181 gtcttccatg ctgccgctct tggcggcgtc gatgctggcg agcttgtcca ggatggcccg
      241 ctcggcggcg atcatcggga cgatatcaag gcgagtgatg cgcttgacgt cgtcgatcgc
      301 caggacgttg agcgggtcgg ccatcgcgag gaacagcttg ttgtcgaggc gcgaaacggg
      361 aacgactttg taattgtggg caacgtcgcg cggcaggagt tcgcaaactt ccggcacgat
      421 gccgatgcgg gcgaggttga caggcgccgt gttgaggacg cggcccatgc aaacggtcat
      481 gtcctgctcg gtgacgtagg atttctcgac cacgagcttg agcaggcgcg tgccttcctt
      541 cttttgctgc tcgagcaatt catccacctg cttggcggtc agcaagccat cttcgacgag
      601 tgcgtcggcg atgcgttctc cgaaagattt tacaaggggt ggcatggttg gatcagcgga
      661 aagctttgcg aatgttggtg acgagttccg agggcgtggt cagataccaa agcaaacggc
      721 tcttggtcgc gtcggctatt tctttcgcgg cctggcggtt gaacgggttc gcggtggcga
      781 cgaaaattgt tttgctcatg cggtcgaacg gcatgacgca ccagcggcgg caagcgtccg
      841 ccggaaaagt gcgcgccaga tcaaggtcca cgtcatattt ttcgagcggc agataaccga
      901 agcgggattt ttccacgagc agcttgaggc ttctttccgg aaggatgatt cccttgtccg
      961 caagcaactg gatgaaggga gtgttcacgc cggtgggcgg atgggaaagg tcggttgtgg
     1021 tccagcaaag gtcgaagtcg ccggcggaaa tgagtttgct gtcaacgaac acttttcgca
     1081 tggtcgcgcg gccatcatcc acttccgctt gggggccatg gtctgccttg cttccgcgcg
     1141 catggccgtt gaccggcttg gcggtggaag aggacaggtc gctgatgccc ggaggcgcgg
     1201 cgaaattgtt ggccttggtt tcgatttcct tgagcgcggt ctggacatca aggtcgtccg
     1261 ggaagcgttg gagaatcgtt tcgtattcga ggatggccga cgaaagctgg cccaactgga
     1321 cgtaagcctg ggcgatgcgt ttggaagtgc cgatgacttc cttctcgcgg ccgagcttgg
     1381 aataagcctc cttgaggatt tcgagcgact gataatcctg cggttgagat tgagtaatga
     1441 cctcgaacat ctcaatcgtc tgagacaact gtgattcttc gctcgtagtt aacgtggcgg
     1501 acatgtttgc aatcggattc ggccttctgg ttatgtcgcc accgtcaagg ctggctttta
     1561 ggcccgaaat tctgcttttg gggagcaaat tcgctgccaa ttgcatacat gcaaaatact
     1621 tggcgaaaat tggcggcttt caggcctgaa aaagcggaat cggctgattt tgagacagta
     1681 aagggtgaat tgtaatccat tgcagtggaa cacgcggcgc aaatagcgtt cctgcgtcct
     1741 atgcgacgaa tacgacaaga ttgctttctc cttcatttaa gttaagacct ctctgtgcaa
     1801 aaactgtcgc ccatcatgac accggcgccg ggggaacgtc tccggcgctt tgtgggcgac
     1861 catgtgcggt tcacgttgag cggcggcgca agcgcgctgg cttcgcaagg ttggcgcgcc
     1921 tgcttgcgga cgaatcttgg ccgcgcggaa atgttgtgca acgaaatcct ccaggcgcac
     1981 accaagaaat tgccgtctgc cggcgcttca tggcacgacc tgccgatgcg cgaggaaaac
     2041 ggcgaatggc atttggaatt gccatgcact gaaccgggct atttcaaatc gaaggcgtat
     2101 ctcatcgatc cgcagggttg gcagcattgg ccggatggtc ctgacttcgg catttccgtt
     2161 cacccggaca gctatcgcac ggcgaacaca atttattgcg ccttcacgcg gatgttcggc
     2221 cccacgaaga atgctctgcg caccgccgac gaaaaattgg agcaacaatt gcggcaattg
     2281 gatgagaaag gttacacggt catcccgccg tcgggaacat tccgcgatct catccaacaa
     2341 ttgccgcaca tcatcgaccg gctcggctgc cgcatcctgc acctgttgcc ggtcaatccc
     2401 acgcccacgg tatcggcgcg gttcggtcgt tttggcagtc cgtatgcgag cttggattta
     2461 acgggaattg atccggccct ggtggttttc gaccgccgca caacgggtgt ggatcaattc
     2521 cgcgaactga cctacgcaat gcatctcaag ggcgggcgcg tttttttgga catggtgatc
     2581 aatcacaccg gctggggctc gaacttgcag gagaatcatc ccgaatggtt tttacgcgat
     2641 gcgaagggaa cattcgtcag tcccggcgca tggggaacga cgtgggagga cttggtcgaa
     2701 cttgagcaca agcatgtcga gttgtgggat tatctggcgg aggttttttt gacgtggtgc
     2761 gaacgcggcg tggatggttt tcgttgcgat gccggttaca aagtgccgtt ggccgcgtgg
     2821 caatacatca tcgcgcgagt ccggcgggaa tttcccgaaa ccattttcct gctcgaaggc
     2881 ctaggcggtt cgtgggaagt cacggagttg ttgctgacgg aaggcgggat gcaatgggcc
     2941 tactcggaac tttttcaaaa ttattcgggc cagcaggtct caagttatct cgattacgcc
     3001 ctcaagcaaa gccagcgcgc gggtctgttc gtccattaca gcgaaacgca tgacaacgag
     3061 cgcctcgcgc gattggggcg taaatggtcg ctgttgcgaa atcgtctct
//
LOCUS       DASZMM010000306         9930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1508590, whole genome shotgun sequence.
ACCESSION   DASZMM010000306 DASZMM010000000
VERSION     DASZMM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508590"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="VG754_07355"
     CDS             complement(<1..1869)
                     /locus_tag="VG754_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF19067.1"
                     /translation="MLNWIVHFSLRFRGVIVALACLTIGYGIYITAHAKLDVFPNFVP
                     PEVTVQTEASGMSSEQVEALVTVPVENTINGLGNMETMRSESIQGLSAITVVFSEGSD
                     IFRSRQMLAEKLNEIASQLPLGVKAPQMSALTSATMDLLKVGLVSDKVSPMELRTFAD
                     WTIKPRLLAVPGVARCIVFGGDVRELQVQVIPERLMAYNLSLSDVLNAARASSAVMGG
                     GYIETENQRIVIQTEGEAVTPEILGEVEVAHHENQSVRLRDIAFVREAAEPKFGDTLI
                     QARSGVLLSMASQYGANTMEVTRAVEAALTELQPIFDREGVKVYPRLHRPATFIEISL
                     RNIKHSLYLGGILVAVVLFLFLGHFRTAFISLTAIPLSLLTAIIIMDKLGVTLNTITL
                     GGLAIAIGEVVDDAIIDVENIFRRLRENKSLPKPRSTFRVVFDASIEVRSAVVYATFI
                     VALVFLPVLTLTGLQGSFFAPLALSYILAIMASLAVALTLTPALSLIFFAKGVKDGDE
                     PRLQTWLKRVYGGILNFFARWPRAVMLVVALICVGALLALPHFGSEFLPEFREGHFVL
                     QIQMTPGTSLGEMRRLGAGISNELLKIKSIATVEEQIGRAESGEDTWGTHRCEFHVE"
     gene            complement(1880..2794)
                     /locus_tag="VG754_07360"
     CDS             complement(1880..2794)
                     /locus_tag="VG754_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19068.1"
                     /translation="MHAADTNAPPAKEEPRITRDTNGNVVVKVDAATQKLIAFKVQPL
                     AAAQLAPELKAYGRVLDPAPLVALMNELASAQASYEVSSNELARLKNLSASGNASARA
                     LQAAEAAELHDRLTTQSARDRLVLAWGKWVVDQPDMPAFIQDLTALSNALIRVDLSAA
                     QHLAAQPLGARVVALSGLSANADVLGRAPGVDPQIQGQGIVLQVKTNESHFLVGEAVT
                     GFLKTPGDTLSGVIVPRSAVVRANGAAWVYAQTADEQFTRKEIALDHPVEGGWFVTEG
                     VAAGDKLVIAGAQTLLSEELKGAFGPPD"
     gene            complement(2842..4218)
                     /locus_tag="VG754_07365"
     CDS             complement(2842..4218)
                     /locus_tag="VG754_07365"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF19069.1"
                     /translation="MLVLLAGCARFTPRPISPAQSAAELDARTLDNPAFRQFLEKNLH
                     RRFDIWPPQSWDFETLNLAALYYHPSLAVARAQWEGAEGGEKTAAQRPNPVVSAVPGY
                     SANPQGPTPWLPGVNFDIPIETMGKRKYRKARAEHLSESARLNIATTAWQVRSNLRTS
                     LIDNASARQRQDLLQKQVSLQQQIVESLLERLEEGEISSSELSLVRIALARAQLDLAD
                     ARRLSADARARVADAIGVPVKALDGVELDYELSASHPTAEQLMSAELRGWALQNRADV
                     LGALADYAASQSALQLEIAKQYPDIHLGPAYQYDEADNKFSLAITAELPILSQNQGPI
                     AEAEAHRTEAAAKFAALQARVISDIDRAVVGYRVSQENLATLGALATAQKKQSDSVQA
                     QVQAGAADRLDLLNSQIELSASALIQLDGQVRAQQAFGALEDAIQRPIEAVSPALTSA
                     QPKKETKP"
     gene            complement(4355..4954)
                     /locus_tag="VG754_07370"
     CDS             complement(4355..4954)
                     /locus_tag="VG754_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWF19070.1"
                     /translation="MKNQTIKNLVKSSSLIALFALAPMLVQAHPGHGLANSFVGGFSH
                     PLFGLDHILAMVAVGLWASQLGGRSVWAVPATFVGLMCVGGALGMMGVHLPLVEAGIL
                     ASVLVLGILIAAAARLPLAASMALVGLFAIFHGHAHGTEIPIAASGLNYGIGFVLATA
                     ALHLCGIGLGFLAQKQLRAPALRYAGAAIAVAGLCLWFT"
     gene            complement(4981..6159)
                     /locus_tag="VG754_07375"
     CDS             complement(4981..6159)
                     /locus_tag="VG754_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HWF19071.1"
                     /translation="MGMWIGRNRKARVTYGFDEIALVPGDATINPNEVDTSFQIPRKD
                     GSHITLKIPIIASAMDGVTDVKFCIEMGKLGGLGVINLEGVQTRYENPSEVLEQVVKA
                     DKEEVTTLIQKLYLEPIKEELIGRRIEELKKAGVQAAVSSIPQKAERFAAIAQEAGAD
                     IFVVQSTVSTVRHISTEYKSLELEAFTKKLNIPVIIGNAVTYNVTLELMECGVAGVLI
                     GVGPGAACTSRGVLGLGVPQVTATVDCAAARDKHFKNTGRYVPIITDGGMSKGGDVCK
                     ALACGSDAVMVGSAFARAKEAPGKGNHWGMATPHANLPRGTRIKVGLSGSLKQILFGP
                     ATVDDGSQNLVGAITTCMGNVGAASIRKFQETEIIIAPSIKTEGKLFQTVQSVGMGTR
                     "
     gene            complement(6270..6494)
                     /locus_tag="VG754_07380"
     CDS             complement(6270..6494)
                     /locus_tag="VG754_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19072.1"
                     /translation="MSTDHLILTYVVSLLAALSGLAIYSELRSRRFGPSSSPDHIFRC
                     SKCGLVYTDDPDVTRSRCSQCGRMNDAIEF"
     gene            complement(6496..7290)
                     /locus_tag="VG754_07385"
     CDS             complement(6496..7290)
                     /locus_tag="VG754_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19073.1"
                     /translation="MTAATIEQGWAGRRWLATIFIVMLGQVALVFWLARHQPEFFPQR
                     DTSPTVLVANDDIMQRLALSNPAQFALANRNGFSGGAWLHTQDLEYDSPEWTEPPRPL
                     ALPVQKLASALSEHVNNDLAGTFEAANAPEPQLEPLAPLPELPAQSVLTIEGELASRP
                     LLSAFKLEPVAAPDILSNSVVQIGVNRDGTVFSAILSDGPKDSKEGKAADAVALSLAH
                     SARFQSLPRVDVGGKSPFDDTLQWGRLVFHWRTVPLPATNSTAANQ"
     gene            complement(7287..7712)
                     /locus_tag="VG754_07390"
     CDS             complement(7287..7712)
                     /locus_tag="VG754_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWF19074.1"
                     /translation="MKFPRNAKIFRGHLDVAPFAGVFFCLLIFVLLGSLVYTPGVHIN
                     LPQSSSTLAGVDGPVLAVAVDPNGQFYFQNQLVQGTNLQQRLQMEAARQSQPLTLVVL
                     ADKSVTQGQLNQLYELARAAGIRQIQEAVLPRAFDSSVP"
     gene            complement(7690..8343)
                     /locus_tag="VG754_07395"
     CDS             complement(7690..8343)
                     /locus_tag="VG754_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWF19075.1"
                     /translation="MLPSFLFNGGPMLWVILFASAAAITVFIERALHCHREQINSTEF
                     LNGVRTVLKRDNVVEALSICDATPGPVARLVKTAILNRDYGRERVRESLEEAGLVEVP
                     RLEEKLNLLATIAQLSPLLGLLGTVLGLIHIFRNMEGAGLTANVGMLSSGVWEALICT
                     AAGLAVSIPAQAGYNYLVSRVNDIVLDMEKAATEIVNIVAEEPGGSIKKTNEIPAQR"
     gene            8571..9239
                     /locus_tag="VG754_07400"
     CDS             8571..9239
                     /locus_tag="VG754_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19076.1"
                     /translation="MRSLTQPKVLGRALVAALITSFACYPRLATWTERGASASFLWAV
                     LLWAMFVLWGFVFAWHFQYSQRPVFGLEKFDPKLWAVASLVAIAAALFVHFNLDPKLR
                     LLTPGTYPTDWNSWVAMGLFALAFEPLFLYFAPFAFFIRLMRRQDAALAMTVVFGLFV
                     LALRINASPAPASVWLLVELVCLRVLGGFAGLYLYIKGGALLVWWVVIILHLRFLLDF
                     AGAG"
     gene            9289..9363
                     /locus_tag="VG754_07405"
     tRNA            9289..9363
                     /locus_tag="VG754_07405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9321..9323,aa:Val,seq:tac)
     gene            9518..>9930
                     /locus_tag="VG754_07410"
     CDS             9518..>9930
                     /locus_tag="VG754_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19077.1"
                     /translation="MTAFIAFIANARAQLPPPPDYTNALWNQAYQGHWYTSGYGHYFC
                     IIHDMEGYYESTISYFQQSGTQASIYYCVNSLHNGSDNKGHAENNPNDAPAGEITQIV
                     REAYWAWHVICWNPWMFGTEHEGFVDSPVWYSEAM"
BASE COUNT         2111 a         2889 c         2811 g         2119 t
ORIGIN      
        1 ctccacatgg aattcgcaac gatgcgttcc ccatgtgtct tcacccgatt cagcgcggcc
       61 gatttgttcc tccaccgttg cgatgctctt gattttcagc agttcattgg atattcccgc
      121 gcccagtcgc cgcatctcac ccaatgacgt gcccggcgtc atctggattt gcagcacaaa
      181 atggccctcc cggaattcgg gtaaaaattc gctgccgaaa tgcggcaacg ccagcagcgc
      241 gcccacacaa atcaacgcca caaccagcat cactgcgcgg ggccagcgcg cgaagaaatt
      301 caagatgccg ccataaacac gtttcaacca tgtttgcaaa cgcggctcgt caccgtcttt
      361 cacgcctttg gcaaaaaata tcagcgacag ggcgggggtc agcgtgagcg ccacggccag
      421 agatgccatg atggcgagga tgtagctgag cgcgagcggc gcaaaaaaac ttccctgcaa
      481 accggtcagc gtcagcaccg gcagaaaaac cagcgccaca atgaatgtcg cataaacgac
      541 tgcgctgcga acttcaatcg aggcatcgaa caccacgcgg aaagtggagc gcggtttggg
      601 cagggatttg ttttcgcgca gacgccggaa aatattttcc acgtcaatga tggcgtcgtc
      661 caccacttcg ccgatggcga tcgccaggcc gcccagcgtg atggtgttca aggtcacacc
      721 gagtttgtcc atgataatga tggcggtgag cagcgagagc ggaatggccg tcaacgaaat
      781 gaatgcggtt cggaaatgac caagaaaaag gaacaacaca acggccacca aaatgccgcc
      841 aagataaagg gaatgtttga tgttcctgag cgaaatttca atgaacgtgg cgggacgatg
      901 cagccgcgga taaactttca cgccttcgcg gtcaaaaatc ggctggagtt cggtcaaagc
      961 cgcttcgacc gcgcgggtga cttccatggt gttcgcgcca tactggcttg ccatggagag
     1021 cagcacgccg gaacgcgcct ggatgagcgt atcgccaaat ttcggctccg ccgcttcgcg
     1081 cacgaaggcg atgtcccgca accgcacgct ctggttttcg tgatgcgcca cttcgacttc
     1141 accaaggatt tccggcgtca ccgcctcgcc ttcggtctgg atgacaatgc gctggttctc
     1201 agtttcgata tagccgccgc ccatgaccgc cgacgaagcc cgcgccgcgt tcagcacatc
     1261 actcagggac aaattatatg ccatcaaccg ttccggaatg acctgcacct gaagttcccg
     1321 cacgtcaccg ccaaatacaa tgcaacgcgc cacacccgga acggccagca aacgcggctt
     1381 gatcgtccag tccgcgaacg tgcgcaattc catcggcgaa actttgtcgg aaaccaggcc
     1441 gaccttcagc aaatccatcg tcgcggaggt gagcgccgac atttgcggcg ccttcacgcc
     1501 aagcggaagc tggctggcga tctcattcaa tttttcggcc agcatttgcc gggagcggaa
     1561 gatgtccgag ccttcgctga acacgacggt gatggccgac aatccttgga tggattcgga
     1621 acgcatcgtt tccatgttgc cgaggccgtt gatggtgttt tcaacgggaa cggtgacgag
     1681 cgcttcgacc tgttcggaag acatgccgga cgcttcggtt tgcaccgtga cctcgggcgg
     1741 gacgaagttg ggaaagacat cgagcttcgc atgagcagtg atgtaaatgc cgtaaccgat
     1801 ggtcaggcaa gcgagcgcca cgatgacacc gcggaagcga agggaaaaat gaacgatcca
     1861 gttcagcatg atgcgcgcct caatccggag gaccgaatgc tcctttcaat tcttctgaaa
     1921 gcagagtttg cgcgccggca ataaccagct tgtcgcccgc cgcgacgccc tcggtcacaa
     1981 accagccgcc ttcaaccgga tggtcgagcg cgatttcttt ccgcgtgaat tgttcatcgg
     2041 cagtctgcgc ataaacccag gccgctccat tcgcgcgcac aacggcggaa cgcggcacaa
     2101 tgacaccgga caatgtgtcg ccgggcgttt tgagaaatcc ggtcacggct tcgccgacca
     2161 ggaaatgcga ttcattcgtc ttgacctgaa gcacaatccc ctgcccttgt atttgcgggt
     2221 caacgccggg cgcgcggcca agcacatcgg cattcgcaga aagtccggaa agcgccacga
     2281 cgcgcgcgcc gaggggttgt gccgcgagat gttgcgcggc ggacaaatca acgcggatga
     2341 gcgcgttgct gagcgcggtc aaatcctgga tgaacgcagg catgtcgggt tggtcaacga
     2401 cccatttgcc ccaggccaaa accagccggt cgcgcgccga ttgcgttgta aggcgatcgt
     2461 gcaattcagc cgcctcggct gcctgcaacg cgcgcgccga agcgttgccg gacgccgaca
     2521 ggtttttcaa acgcgccaat tcattgcttg aaacctcata tgaggcttgt gcggatgcca
     2581 gttcgttcat caacgcaacg agcggcgcag ggtcaagcac gcggccataa gccttgagtt
     2641 ccggggcaag ttgcgctgcc gccagcggct ggaccttgaa cgcgatgagt ttctgggtcg
     2701 ctgcgtccac tttgacaacc acgttgccat tggtgtcgcg cgtgatccga ggctcttcct
     2761 tggcgggcgg ggcgttcgtg tcggctgcgt gcacattgca aaggaaagaa aggattaacg
     2821 ggataaaaaa tattgctcgt ttcacggttt ggtttctttc tttggctggg cggaggtcaa
     2881 ggccggtgag acggcttcaa tcgggcgctg aatggcgtct tccaatgcgc cgaatgcctg
     2941 ttgcgcgcgc acctggccat ccagttgaat cagcgcgctg gcgctgagtt cgatctgaga
     3001 attcaacaag tcaaggcggt ccgccgcgcc tgcctggact tgcgcctgca cggagtcgct
     3061 ctgttttttt tgcgccgtcg ccaatgcgcc gagcgtggca aggttttcct gcgaaacgcg
     3121 ataaccgaca acagcgcggt cgatgtcgga aatcaccctg gcctgcaacg cggcgaactt
     3181 cgccgccgct tccgtgcgat gcgcttcggc ttcggcaatg ggtccctgat tttggctcaa
     3241 gatgggcagt tccgcggtga tcgccagaga aaatttatta tcggcctcat cgtattgata
     3301 agccggcccg agatggatgt ccggatattg cttggcgatt tccagttgca aggccgactg
     3361 gctcgcggcg taatcggcca gcgcgcccaa aacgtccgcg cgattttgca acgcccaacc
     3421 gcgaagttcc gcggacatca actgctccgc cgtcggatgg cttgcggaaa gttcgtaatc
     3481 gagttcaacc ccgtcaagcg ccttcaccgg gacgccgatt gcatccgcaa cgcgcgcgcg
     3541 agcatcggcg ctgagacgcc gcgcatcggc aagatcaagt tgagcgcggg cgagcgcgat
     3601 cctgacgagg ctcaattccg aactggaaat ttcgccttct tcaagacgtt ccagcaaaga
     3661 ttcgacaatc tgctgttgca aggaaacttg tttttgcaac aaatcttgcc gttggcgcgc
     3721 gctggcatta tcaatcaggc tcgtgcgcaa attgctccgc acctgccatg cggtggtggc
     3781 gatgttcagg cgcgccgatt cggaaagatg ttcagcgcgc gctttgcggt atttgcgctt
     3841 gcccatggtt tcaatcggaa tgtcaaagtt cacgcccggc agccagggcg tcgggccttg
     3901 gggattggcg ctgtagcccg gcaccgcgct gacgacggga ttgggccgtt gtgcggcggt
     3961 tttttcaccg ccctccgcgc cttcccattg cgcgcgcgcg accgcgaggc tgggatgata
     4021 ataaagcgcg gcaagattca gcgtttcaaa atcccatgat tgcggaggcc agatgtcaaa
     4081 gcggcggtga agattttttt cgaggaattg ccggaacgcg ggattgtcca gcgtgcgtgc
     4141 gtccagttca gcggcggatt gcgcgggcga aatgggacgc ggggtgaatc gcgcgcaccc
     4201 ggccagcaaa acgagcatta tgaggcacca tttcaggttc aagctcgttt acttcaagtg
     4261 aaagcacgcc ggaatgcaag cattccaaaa ttaaattttt ctcacctaaa aatgtccgct
     4321 gatccacttc gtatccagat gacgaggcgt tctattacgt gaaccagagg cacaggccgg
     4381 cgaccgcaat ggccgcgcct gcgtaacgaa gcgccggtgc gcggagttgc ttttgggcca
     4441 ggaagccaag gccgattccg cagaggtgaa gcgcagccgt ggccagcaca aaaccgatac
     4501 cataattcaa accgctcgcg gcaatgggaa tttcagttcc gtgcgcatga ccgtgaaaaa
     4561 tcgcgaacaa accgaccaac gccatgcttg cagccagcgg cagacgcgcg gcagcagcga
     4621 tcaaaatccc cagtacaagc acagacgcca aaatgcccgc ttcaaccagc ggcagatgca
     4681 cacccatcat tcccaaagcg ccgcccacgc acatcaaacc gacgaacgtc gcgggaacgg
     4741 cccacacgga gcgtccgccc agttgcgatg cccaaagccc gacggccacc atggccagaa
     4801 tatggtccaa gccgaaaagc ggatggctga atccgccgac aaaactgttc gccaagccgt
     4861 gcccgggatg cgcctgaacc agcatcggcg cgagcgcgaa cagcgcgatc aaggacgacg
     4921 acttcaccaa gtttttgatg gtctgatttt tcatagtttt ttgggttgaa tttccgatgc
     4981 tcaacgcgtt cccatgccga cgctttggac ggtctggaaa agcttgccct ccgtcttgat
     5041 gctgggggcg atgatgattt ccgtttcctg aaatttgcgg atgctcgccg cgccgacatt
     5101 gcccatgcaa gtggtgatcg cgccgacgag attttgcgag ccgtcgtcca ccgtcgcggg
     5161 gccgaacaaa atttgcttga gcgaaccact gaggccgacc ttgatgcgcg tgccgcgcgg
     5221 caagttggcg tgaggcgtcg ccatgcccca atgattgccc ttgcccggcg cttccttcgc
     5281 gcgcgcaaaa gcgctgccga ccatgaccgc gtccgaaccg caagcgagcg ccttgcaaac
     5341 atcgccgccc ttgctcattc cgccgtcggt gatgatgggc acgtatctgc cggtgttctt
     5401 gaaatgcttg tcgcgcgcgg cggcgcaatc caccgtggca gtgacttgcg ggacgcccaa
     5461 accaagcacg ccgcgactgg tgcatgccgc gccgggaccg acgccgataa gcacgcccgc
     5521 cacaccgcat tccatcaatt cgagcgtgac attgtatgtg accgcgttgc cgatgatgac
     5581 cggtatgttg agcttcttgg tgaacgcctc gagttcgagc gatttgtatt cagtggaaat
     5641 atggcgaacg gttgagaccg tggattgcac cacgaaaatg tccgcgcccg cttcctgagc
     5701 gatggcggca aagcgctcgg ctttttgcgg aatggaactg accgcagcct gcacgccggc
     5761 ctttttcaat tcctcgattc gcctgccaat caattcctcc ttgatcggtt caaggtaaag
     5821 tttctggatc aatgtggtga cttcttcctt gtccgccttg accacctgtt cgagcacttc
     5881 ggaaggattt tcgtaacgcg tctggacacc ttcgagattg atgacaccca aaccgccgag
     5941 cttgcccatc tcgatgcaaa atttcacatc ggtcacgccg tccattgcgc tggcgatgat
     6001 gggaattttg agcgtgatat ggctcccgtc cttgcgcgga atctgaaagc tggtgtccac
     6061 ttcgttcggg ttgatggtgg catcacccgg aaccagcgca atttcatcga acccgtaggt
     6121 cacacgggct tttctgtttc ggcctatcca cattcccata aatttttctg ctgctaaata
     6181 aagttgttgg cgggatgtgt tgcatcgccg ccggcgttta tgttgacaaa caatctcgca
     6241 gcacttgcgc cggactgcaa cccccggctt cagaattcga tggcgtcgtt catccggccg
     6301 cactgcgaac agcgggaacg ggtcacatcc ggatcgtcgg tatagaccaa accgcatttg
     6361 ctgcaacgga atatatggtc gggactcgac gacggcccga atcgcctgct gcgtaattcc
     6421 gaatatatcg cgaggcccga aagtgccgca agcaaagaaa ccacgtaggt caaaatgaga
     6481 tggtcagtgg acatgttact gattggccgc ggtcgaattg gttgcgggca acggaacggt
     6541 gcgccaatgg aacactaacc gtccccattg cagcgtatca tcgaagggac tttttccgcc
     6601 tacgtcaact cggggcaaag attggaagcg cgcagaatgg gcgaggctga gcgcgacggc
     6661 atccgcagcc ttgccttcct tggaatcctt cggcccgtcc gacaatatgg ccgagaacac
     6721 tgtcccatcc cggttcacgc cgatttgcac cacgctgttg gacaaaatat ccggcgcggc
     6781 gaccggttcc agcttgaatg cggaaagcag cggacgactg gcgagttcgc cttcgattgt
     6841 gagaacggat tgcgcgggca actccggcaa gggggcaagc ggctcgagtt gcggttcggg
     6901 cgcattggcc gcctcgaacg tgccggccaa atcgttgttg acgtgttcgc tcagggcgct
     6961 ggcaagtttt tgcaccggca aggcgagcgg acgcggcggt tcggtccatt ccggcgaatc
     7021 gtattccaag tcttgggtat gcaaccatgc gccgccagaa aaaccatttc ggttcgcgag
     7081 cgcaaattgc gccgggttgc tcaatgccag ccgctgcatg atatcgtcat tggccacaag
     7141 taccgtcggt gaagtgtccc tctgggggaa aaattcgggt tgatgccgcg caagccagaa
     7201 aacgagcgcc acctggccca gcatcacgat aaaaatcgtg gcgagccatc ggcgacctgc
     7261 ccaaccctgt tcaatcgtcg cggcagtcat ggcacgcttg aatcaaacgc ccgcggcagg
     7321 acggcttcct ggatttgccg gatcccggcg gcgcgcgcga gttcgtaaag ttggttcaac
     7381 tggccttgcg tgactgattt gtcggccagc acgaccaatg tgagaggttg gctttgacgg
     7441 gcggcttcca tctgcaatcg ctgctgcaaa ttcgtccctt gcacgagctg gttttgaaaa
     7501 taaaattgcc cgttgggatc cacagcgacc gccagcaccg gcccgtccac accggccaga
     7561 gtggaggaag attgcggcag gttgatgtgc acgcccggcg tgtaaaccag cgaaccgagc
     7621 aaaacgaaaa tcaacaggca gaaaaaaacc ccggcaaatg gcgccacgtc cagatggccg
     7681 cggaatattt tagcgttgcg cgggaatttc attggttttt ttgatggagc cgcccggctc
     7741 ttcggcgacg atgttcacga tttcagtcgc ggccttttcc atgtcgagga cgatgtcatt
     7801 cacgcggctg accagataat tataaccggc ctgcgccgga atcgaaaccg ccagtcccgc
     7861 agccgtgcag atcaacgctt cccaaacacc gctggaaagc atccccacat tcgcggtcaa
     7921 acccgcgccc tccatgttgc gaaaaatgtg gatgagtccc aataccgttc ccagcaatcc
     7981 aagcaacggc gataattgcg cgattgtcgc cagcaggttc aatttttcct ccagccgcgg
     8041 cacttcgacg agtcccgctt cctccagaga ttcgcgcacg cgttcgcgcc cgtaatcgcg
     8101 gttgaggatg gctgtcttca ccaaccgcgc gaccggtccc ggcgttgcgt cgcaaatcga
     8161 aagcgcctcg accacgttgt cgcgcttcag caccgtgcga acgccgttga gaaattcggt
     8221 ggaattgatc tgctcgcgat ggcaatggag cgcccgctca atgaacaccg tgatggcagc
     8281 cgcgctcgca aaaaggatca cccagagcat cgggccgccg ttgaaaagaa aactcggcaa
     8341 catgtgctta tataaaggtt taaagttgaa agttcaaagt ttaaagtttc ccccaatgcg
     8401 cctcttaata ctgatctgta ccggggactg gaaagtccgg aactcgcaga caagaatgtc
     8461 tgcgctacgt ggcgcaggct ttccggcctg cgggttcagg cgactttcca gtcgccagct
     8521 ttccgactgt agaacccgac aatgctgatc attagcattc actcctctca atgcgctcat
     8581 tgactcaacc aaaagtcctt ggccgcgcgc tggtggccgc gctgatcaca agcttcgcct
     8641 gttatccgcg cctggcaacg tggacggaac gcggcgcttc cgcgtctttc ctgtgggcgg
     8701 tcctgctctg ggcgatgttt gtgctttggg gttttgtttt tgcctggcat ttccaatatt
     8761 cacaacgccc ggttttcggc ctggaaaaat tcgatccaaa actttgggcc gttgcgtcgc
     8821 tggtcgcgat tgcggctgcg ttgttcgtgc atttcaacct tgatccgaaa cttcggctgc
     8881 tcacgccggg aacttatccc accgattgga attcgtgggt ggcgatgggc ttgtttgcgc
     8941 tggcgtttga accgttgttt ttatatttcg cgccgttcgc gttttttatc cggcttatgc
     9001 gtcggcagga cgccgcgctg gcgatgaccg ttgtgttcgg actttttgtg ctcgcattaa
     9061 ggatcaacgc gtcccctgcc ccggcatccg tctggctgct ggtcgaattg gtgtgtttgc
     9121 gcgtgctggg cggttttgcg ggattgtatc tttatatcaa aggcggcgcg ctgctggtct
     9181 ggtgggtggt gatcattttg catcttcgtt ttctcctgga ttttgccggg gcgggttgaa
     9241 acaaatccgc ttgcgtcggc tgcttttccg cgcaagatag tatgagtcgg gcgattggcg
     9301 cagtggtagc gcagcagctt tacacgctgt gggtcggggg ttcgaatccc tcatcgccca
     9361 ccatctcttt ccccaatcaa taaaaaaagc tcgcctgaaa acccggtttg tggcaccatc
     9421 cacgccgttc cagttcaatc agaaaaaccc tatgtcatgc cctgaacaca tccgtgtcct
     9481 cattgcgcgc aaatcttttc ttcgccggat ttttttgatg actgctttta ttgcgttcat
     9541 cgcgaatgcc cgcgcgcaac ttcctccgcc gccggattac acgaacgcgc tctggaacca
     9601 agcgtatcaa ggccattggt acacctcggg ttacggccat tatttctgca tcatccacga
     9661 catggaaggt tattacgaat cgacaatctc gtatttccaa caatcgggca cccaagccag
     9721 catttattat tgcgtgaaca gcctccataa cggctctgat aacaaaggcc acgctgagaa
     9781 caatcccaac gacgcgccgg ccggcgaaat cacccaaatt gtccgcgagg cgtattgggc
     9841 gtggcacgtc atttgttgga atccctggat gttcggcacc gagcacgaag gttttgtgga
     9901 cagccccgta tggtattccg aagccatgta
//
LOCUS       DASZMM010000307         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5063379, whole genome shotgun sequence.
ACCESSION   DASZMM010000307 DASZMM010000000
VERSION     DASZMM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5063379"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VG754_07415"
     CDS             <1..1534
                     /locus_tag="VG754_07415"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19078.1"
                     /translation="NPYMIQVGGTTLTTSGPGGAWVSETAWNWGGGSATSGGVSPIYT
                     IPSWQEGVNMTFNAGSATHRNFPDVAMAADNIFIVADNGQQESVGGTSCAAPLWAAFT
                     ALVNQQAAVTGKNPVGFINPAIYNIGLSSAYGSDFHDVTSGNNTNSLCLAEFYAGTGF
                     DLCTGFGTPTGSNLMTALLAPADALQIIPISGFTSCGAVGGPFNITAQTYTLTNTGSA
                     SLNWSNAITATWLNVSPASGTLAAHANATITVSLTAAANSVSAGNYSGNVWFTNLTSH
                     FAQMRQFALRAGQTLVRNQGFETGDFSYWLLGGDNGTYNFVDNGSITGITPHSGSYCA
                     TLGQVGLPLATLSENVPTFSGQPYLLSFWLKNPNIGSGTTPNQFSVAWNGVTLLNKTN
                     MATQSSWTNFQFLVGATNTSSALQFGFRNDNSAFGFDDVTVTSFPQPAFTKVLETNRV
                     IKLTWSAVSNYNYQLQYATNLFQTNWAALGGTIKATNSTMNSTDSTTNNAQRFYRVVL
                     IL"
     gene            complement(1558..>3091)
                     /locus_tag="VG754_07420"
     CDS             complement(1558..>3091)
                     /locus_tag="VG754_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HWF19079.1"
                     /translation="GLFSQPQSIVPKWGRYEQQFRSATNYPNPLEDATLKVYLTSPLG
                     ENFTTYGFWDGGKVWRVRFSPNFPGKWKFRTVCSDAANQGLHNITGEFTCTVEDKRSA
                     FDRHGAVRVARDNRHFEYEDHTPFFWLADTVGNGAIRSTLKDWQSYAQVRSAQKFTAV
                     EWQALEEKADESAVFLKAGHLDVNPEYFRKLDAKADALNRAGLLDAIIPFTKGTMAGT
                     NSVDALPEEQAIALLRYFVARWDANDVAWLLPCDDARPGRWKRIGRAVFGEITHAPVV
                     VNSGEASWMLDELRAEPWVNALGYQCSQDMTDDALQLMIAGPVTKDWPKEPTRPLINL
                     ALPRENQAQPSDMLRRAAYWSFLSAPLAGVTFVNATVSTWDTTVNSNSVDDRQAWQTA
                     LLSPGATNMGILADAFSSIAFWRLRPATDLLVGQNAPDMTYIAAARSEESDLAAVYVP
                     QSKPVELNVKPLPKSTAAIWIDPRTGQRSSVAFSPSGPTEKMLPPSGSHDWLLFIAGS
                     AN"
BASE COUNT          697 a          867 c          826 g          701 t
ORIGIN      
        1 caacccttac atgatccaag tcggcggcac aacgttgaca acttctggac ctggcggggc
       61 gtgggtttcg gagacggcat ggaattgggg cggggggtcc gcgaccagtg gcggcgtcag
      121 tccgatttac acaattccaa gctggcaaga aggcgtcaac atgaccttca acgccggctc
      181 ggcgactcat cggaattttc ccgatgttgc gatggccgca gacaatattt tcatcgtcgc
      241 cgacaacggc cagcaggaat cagtcggcgg cacgagttgc gccgcgccct tatgggcggc
      301 tttcaccgca ttggtaaatc agcaagctgc agtgacgggc aagaaccctg tgggctttat
      361 caatccagct atttataaca ttggcttaag ttcagcttac ggatccgatt ttcatgatgt
      421 tacctcaggc aataatacaa acagcctttg tcttgctgaa ttttatgccg ggactggttt
      481 cgacctttgc accgggtttg gcacgccgac cgggagcaat ttgatgaccg cgctgctcgc
      541 gccagccgat gccttgcaaa tcatcccgat ttccggcttc acatcctgcg gcgccgtggg
      601 aggaccgttc aatatcaccg cgcaaactta caccctgacc aacaccggaa gcgcttcgct
      661 gaattggagc aacgcaatca ccgcgacatg gctcaatgtc tcacccgcca gtggaacatt
      721 ggcggcgcat gcaaacgcga cgatcacggt gagtctgacg gccgctgcca acagtgtttc
      781 cgccgggaat tattctggaa acgtctggtt cacgaatctt accagccatt ttgcccaaat
      841 gcggcagttc gctttgcgcg cgggacagac tttggtccga aatcaaggtt ttgaaactgg
      901 agatttttcg tattggttgc tgggcggcga taatgggact tacaattttg tggacaacgg
      961 gtccatcacg ggaatcacgc cgcactcggg gagttactgc gcgaccttgg ggcaggtggg
     1021 tttgccgctc gcaacgcttt cggaaaatgt ccccactttt tccggacaac cgtacctgct
     1081 ttcgttctgg ttgaaaaatc cgaacattgg cagcggcacc acgccgaacc aattttctgt
     1141 tgcctggaac ggcgtcacac ttctgaataa aaccaatatg gccacgcaaa gcagttggac
     1201 caattttcaa ttccttgtgg gggccaccaa caccagcagc gcgctccagt tcggttttcg
     1261 aaacgacaat tccgcctttg gcttcgacga tgtcacggtg acttcgtttc cgcaacccgc
     1321 gttcacaaaa gtgctggaaa ccaatcgcgt catcaagctc acatggagcg ctgtttcaaa
     1381 ttataattac caattgcaat acgcgaccaa tctttttcag acgaactggg ccgctttggg
     1441 cggcacaatc aaggcaacga actcaacaat gaattcaacc gattccacca cgaacaatgc
     1501 gcaacgattc tatcgggttg tgttgatact ttaagtctgc aatttgctct ttgaaaatta
     1561 gttggcgctg ccagcaatga aaagaagcca gtcatggctt cccgatggcg gaagcatttt
     1621 ctcggttggg ccgctcggag aaaatgcgac gctggaacgt tggccggtgc gcgggtcaat
     1681 ccagatggct gccgtcgatt ttggaagtgg cttcacattc agttccaccg gcttgctttg
     1741 cggaacatat actgcggcca aatcagattc ttcagagcgc gctgcggcaa tgtatgtcat
     1801 gtctggagcg ttttgcccga ccagcaaatc tgttgccgga cggagccgcc aaaaggcgat
     1861 cgaactgaaa gcatccgcga ggattcccat gttggtcgcg ccgggagata acaacgccgt
     1921 ctgccacgcc tgccggtcat caacgctgtt gctgttgaca gtggtgtccc aagtagaaac
     1981 tgtggcattg acaaaggtca caccggccaa gggcgcgctt agaaagctcc agtacgcggc
     2041 gcgccgcaac atatccgatg gttgggcctg attttcgcgc gggagcgcga gattgatcaa
     2101 tggccgcgtc ggttccttgg gccaatcttt tgtcaccggc ccggcaatca tcaattgcaa
     2161 cgcgtcgtct gtcatgtcct ggctgcattg ataaccaagc gcgttcaccc acggttccgc
     2221 gcgcaattca tcaagcatcc aagaggcctc gccggaattc acaaccacag gcgcgtgcgt
     2281 gatctcaccg aacaccgcgc gcccaatccg tttccaacgg ccgggacgcg cgtcgtcgca
     2341 cggcaacagc caggcaacat cgtttgcatc ccaacgcgcc acgaaatagc gcaacagtgc
     2401 aattgcttgt tcctccggca gggcatccac ggaatttgtg ccggccattg ttcccttggt
     2461 aaaaggaatg atggcgtcga gcaacccggc gcggttcaac gcgtccgctt tggcatcgag
     2521 tttgcgaaag tattctggat tgacatccag atgtccggct ttcagaaaca ccgcggattc
     2581 gtccgccttt tcctcgagag cctgccattc aaccgccgtg aatttttgtg cggaacggac
     2641 ttgcgcataa gattgccagt ccttcagcgt ggaacggatc gcgccgtttc ccacagtgtc
     2701 agcaagccag aaaaatggcg tgtgatcttc atattcgaaa tgccggttgt cccgcgccac
     2761 gcgtactgcc ccatgacggt caaacgccga tcgcttgtcc tcaacggtgc aggtgaattc
     2821 gccggtgata ttgtgcaagc cttggttcgc agcatccgag caaaccgtgc gaaacttcca
     2881 cttgccgggg aaatttggcg agaaacggac gcgccagacc ttgccgccgt cccaaaatcc
     2941 atacgtcgta aaattctcgc ccaatggcga agttaaatag accttgagcg tggcgtcctc
     3001 cagcggattg ggataattgg tggcgcttcg aaattgttgc tcgtaacggc cccacttggg
     3061 aacgatggat tgcggctgtg aaaaaagacc t
//
LOCUS       DASZMM010000308         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3977608, whole genome shotgun sequence.
ACCESSION   DASZMM010000308 DASZMM010000000
VERSION     DASZMM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3977608"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VG754_07425"
     CDS             complement(<1..396)
                     /locus_tag="VG754_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWF19080.1"
                     /translation="MNPAEIPELKQSSLYSPDLAPVPPEGRKWKMWNFAALWISMAAC
                     IPTYMLASGLIGEGMNWSQAIFTIFLGNLIVLIPMVLNAHAGTHYGIPFPVFCRAAFG
                     TVGANVPALLRALVACGWFGIQTWIGGNAI"
     gene            521..733
                     /locus_tag="VG754_07430"
     CDS             521..733
                     /locus_tag="VG754_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF19081.1"
                     /translation="MKFRVIIQPDEDGLFVAECPTLPGCISQGETRAEAMANIKDAIE
                     GYLASIKKHGEPIPSPITEEIIEVAT"
     gene            730..954
                     /locus_tag="VG754_07435"
     CDS             730..954
                     /locus_tag="VG754_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWF19082.1"
                     /translation="MSKLPVCSGHEAVRAFRQIGYEVDHQTGSHIILRHANMRRLTIP
                     NHRELAKGTLRAIIREAGITKEEFIDLLKS"
     gene            complement(959..>2152)
                     /gene="hydA"
                     /locus_tag="VG754_07440"
     CDS             complement(959..>2152)
                     /gene="hydA"
                     /locus_tag="VG754_07440"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWF19083.1"
                     /translation="LPFMGTFSKDTYETGSKAALVGGTTTLIEMCCPSRKEEALAGFE
                     TWMAQAVGKSACDFTFHMGVTKFDANSEKQLREIVSRGISSFKIFLAYKGAFGIDDSE
                     LYHTLKLAKELGVIVTAHCENEILVAERQKELLAAGKTDPGQHHESRPPAVEAEGVHH
                     LMTFAELTGAATYIVHLSCKEALDEAIAARQRGVRVNVETLIQYLVLDKTYAEQPGFE
                     GAKYVMSPPLRDVRNQAVLWNGLRSCLVNTVATDHAPFDFQSQKPMGKDDFTKIPNGI
                     PSLEERVNLLYTRGVKTGLIDLHTFVNVASTAVAKLFDLFPRKGTIQPGSDADLVVYD
                     PNYHGTISAKTQMMNVDYSAFEGWPVEGRPSVVTVRGEVAVHDGKFVGQIGRGQFLKR
                     TPSHF"
BASE COUNT          499 a          601 c          577 g          475 t
ORIGIN      
        1 aatcgcgttg ccgccaatcc atgtctggat gccgaaccat ccgcacgcca ccaatgcgcg
       61 caaaagtgcc gggacgtttg cgcccacagt gccgaatgcc gcgcggcaaa aaaccggaaa
      121 cggaatccca taatgcgtgc ccgcgtgcgc gttcaacacc atcgggatca gcacgatgag
      181 gttccccaag aaaatcgtga agatggcctg cgaccagttc atcccctcgc cgattaagcc
      241 ggacgcaagc atgtaagttg gaatgcacgc cgccatggaa atccagagcg cggcgaaatt
      301 ccacatcttc cacttgcgcc cttccggcgg cacaggggca aggtcgggac tatagagcga
      361 gctctgtttc aactccggga tttcggcagg attcatgcgc gtgaatgcca ctgttttgcg
      421 gccatttaat ttataaaacc cgcaatctga ttgaatagca agcgctggat gccgagcctg
      481 aacagttgac cggagtgatt caataattta cgatttaacc atgaaatttc gggtgattat
      541 ccaaccggat gaagatggcc ttttcgtagc cgaatgtccc acattgccag gctgcatctc
      601 ccaaggggaa actcgcgcgg aagccatggc taacatcaag gatgcaattg aaggatacct
      661 ggcaagcatc aaaaagcatg gtgagccaat cccatcacca attaccgagg aaatcataga
      721 ggttgcgacg tgagcaagtt gccggtgtgt tccggccacg aggccgtgcg cgcatttcgg
      781 cagattgggt acgaagtgga tcatcagact ggaagccaca tcattttgcg ccacgccaac
      841 atgcgccggt taacaattcc caatcaccga gaactggcta aaggaacctt gcgagcaatt
      901 attcgggaag caggcatcac caaggaagaa tttatagatt tgctcaaaag ttgatctgct
      961 aaaaatgact cggagtccgt ttgaggaatt ggccgcgtcc aatctgcccc acaaattttc
     1021 cgtcgtgcac tgcaacttct ccacgaaccg tcaccacgct cggacggcct tcaaccggcc
     1081 agccttcgaa cgcgctgtaa tccacgttca tcatttgcgt ttttgccgaa attgtcccat
     1141 gataattcgg atcatagact accaaatccg cgtcgctgcc tggctggatg gtgcctttgc
     1201 gcggaaacaa gtcgaacagc ttcgccactg ccgtgctcgc cacgtttacg aacgtgtgca
     1261 gatcaatcaa gccggtcttc acgccgcgcg tgtaaagcag gttcacgcgc tcttccaatg
     1321 atggaatgcc gttggggatt tttgtgaagt cgtccttgcc catcggtttt tgcgattgaa
     1381 aatcgaacgg cgcgtggtcc gtggcgacgg tgttgacgag acaggaacga aggccattcc
     1441 ataacaccgc ctggttgcgc acatcgcgca atggcggaga catcacgtat ttcgcgccct
     1501 caaaaccggg ctgttcggcg taagtcttgt cgagcacgag atattggata agcgtttcga
     1561 cattcacccg cacgccgcgt tgacgtgctg cgatggcttc atccaaagcc tccttgcaac
     1621 tgagatgcac aatgtaagtg gccgcgccgg tcaactcggc aaaagtcatc aggtgatgaa
     1681 caccttccgc ctcgaccgcc ggcgggcggc tttcgtgatg ctggcccgga tcggtcttgc
     1741 cggcggcgag cagttccttc tggcgctccg caaccaatat ttcgttttca caatgcgcgg
     1801 tgacgatgac gcccagttcc ttcgccaatt tgagcgtgtg atacagttcg gagtcgtcaa
     1861 tgccgaacgc gcctttatat gcgaggaaga ttttgaagga gctgatgccg cggctgacga
     1921 tttcgcgaag ctgtttttcg ctgttcgcgt caaatttcgt cacgcccatg tgaaacgtga
     1981 aatcgcaggc ggatttgccg accgcctggg ccatccaggt ttcgaaaccg gccagcgctt
     2041 cttccttgcg cgacggacag cacatctcga taagcgtggt ggtgccgccg accagcgcgg
     2101 ctttgctgcc ggtttcatag gtgtccttgg aaaacgtgcc catgaacggc ag
//
LOCUS       DASZMM010000309         5988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5065107, whole genome shotgun sequence.
ACCESSION   DASZMM010000309 DASZMM010000000
VERSION     DASZMM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5065107"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /gene="rplQ"
                     /locus_tag="VG754_07445"
     CDS             complement(<1..356)
                     /gene="rplQ"
                     /locus_tag="VG754_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWF19084.1"
                     /translation="MRHLKRTAKLGRTGTHRNAMLANLVCSLIKHKRVTTTLAKAKAA
                     RSVAEKMVTLGKSGTVHDRRLAAARLHQEDAVKILFNDIAPVQKDRHGGYTRIVRLGE
                     RQGDASQLAILEWVDVS"
     gene            complement(366..1397)
                     /locus_tag="VG754_07450"
     CDS             complement(366..1397)
                     /locus_tag="VG754_07450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWF19085.1"
                     /translation="MPVRLGRFEMPKRLTKEESTATETYAKFVAEPFETGYGHTIGNS
                     IRRVLLSSLEGAAITSIKIDGAMHEFTTVDGVVEDVTDIILNLKKILLKAHSREPQTL
                     LLSVNKEGEVLASDIELNQNVELVNPKQHICTLDKKKKFEMELEVKVGRGFCPGDENK
                     KANQAIGVIAIDSLFSPVTRVRYAVESARVGQRTDYDRLIIELWTDGRISPDDALTQA
                     SAILQHHLDVFVGYDKNAVEFEEVVDKQDEEKTKLKKLLGMSVNEIELSVRAANCLNN
                     ANITTVGQLAMKSEAEMLKYRNFGKKSLNEIKEKLSTLGLTLGMTFDAETLESPKEET
                     GAPVTTTVE"
     gene            complement(1507..2115)
                     /gene="rpsD"
                     /locus_tag="VG754_07455"
     CDS             complement(1507..2115)
                     /gene="rpsD"
                     /locus_tag="VG754_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWF19086.1"
                     /translation="MARYTGPRVRISRRFGTPIFGPTKYLERRNYGPGVHGPKSRRKH
                     TDYGLGLIEKQKLRYYYGLMERQFRGVYERALKRRGVTGEQMLQILETRLDNVAFHLG
                     FATTRSAARQLISHGHVKVNGRKVNIPSFALKVNDVVEIKDSNVSRQLATKNLELATS
                     RAVPDWLSLNKEGFKGVIMRIPTRDEIQPIANEQAVVEFYSR"
     gene            complement(2163..2762)
                     /gene="rpsK"
                     /locus_tag="VG754_07460"
     CDS             complement(2163..2762)
                     /gene="rpsK"
                     /locus_tag="VG754_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWF19087.1"
                     /translation="MADETKKKPAPKKEAKPEGAAEAKAAPAEGAKPKKAAAPAAEGA
                     DAAAPKPAEGAPVAAAAPTAAELLADDPNAKKIIKAKGAKNISTGIANILATFNNTQV
                     SITDVHGNLLGWSSAGRVGFKGSRKSTAYAAQQVAQDAARQAMAHGMREVEVRVKGPG
                     SGRESAIRALQAIGLEVTMIRDVTPIPHNGCRPRKKRRV"
     gene            complement(2775..3170)
                     /gene="rpsM"
                     /locus_tag="VG754_07465"
     CDS             complement(2775..3170)
                     /gene="rpsM"
                     /locus_tag="VG754_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWF19088.1"
                     /translation="MARIIGVEIPGEKRIDIALRYIYGIGPVNSKVILEKAKIDPSIR
                     AKDLNEQQMSQIVHAIQDGKYVIEGDLRRELGMNLKRLQGIKCYRGVRHLRGLPVRGQ
                     RTQTNARTRKGPRKTVGVQRNPNAKTGIH"
     gene            complement(3177..3290)
                     /gene="rpmJ"
                     /locus_tag="VG754_07470"
     CDS             complement(3177..3290)
                     /gene="rpmJ"
                     /locus_tag="VG754_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWF19089.1"
                     /translation="MKVRASVKKLCENCKVVKRKGVIRVICTNARHKQRQG"
     gene            complement(3287..3511)
                     /gene="infA"
                     /locus_tag="VG754_07475"
     CDS             complement(3287..3511)
                     /gene="infA"
                     /locus_tag="VG754_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWF19090.1"
                     /translation="MARTDAIEVEGAVIEALPHAMFKVELANGHRVLAHFKGKARANP
                     APLKPGDKVKLEMSLFDLSNGCIIETGKQI"
     gene            complement(3472..4248)
                     /gene="map"
                     /locus_tag="VG754_07480"
     CDS             complement(3472..4248)
                     /gene="map"
                     /locus_tag="VG754_07480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWF19091.1"
                     /translation="MIVLKNERDLEAMRPACMVAGTVLDEIAAFVKPGMTTKDIDLFA
                     ASRIKHYGAKSAFLGYRKYPCNICISVNEQVVHGLANQRRVEFGDIISLDVGVVYNRF
                     IGDTARTVAVGGCSVAAQRLMDVTEKALYEGIAQAIPGNRVTDISRAIQNYVEGNGFS
                     IVREFVGHGVGRSMHEEPQIPNFVDPKNNQKLRPGMTLAIEPMVNAGQPGVKILNDGW
                     TVVTQDGSLSAHFEHTVLVTEAEPEILTWPERMPSKLKAQ"
     gene            complement(4302..5690)
                     /gene="secY"
                     /locus_tag="VG754_07485"
     CDS             complement(4302..5690)
                     /gene="secY"
                     /locus_tag="VG754_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWF19092.1"
                     /translation="MLASIVNTFSNCFKIPELKSRIGFTFMLLAICRLAAFIRIPSID
                     GAALSAYLASHAAGSGGLLNMYSLFTGGALENCAVGALGIMPYISATIVMQLLTAVVP
                     TLSKLAREEGGRVKIIKYSRYLTVLLCIGQGYFMAYSWENPQTIFGGWNQGPLVLSDN
                     IWWYRIETVLILTTGTLLLMWLGEQITERGIGNGISLVITIGILARLPAAFQGLADMY
                     FSPAGKGNFGNGIVLVALLIAVVAGVIAVTQAQRKIPVQYAQRAVGRKVYSGGTSFLP
                     LRLNFAGVMPIIFAQAILMVPQKLFTMIGSNFPSFAFLGKIGNALNYGAMTYLVVYTL
                     MILFFSYFWTATQFNELQIADDLKKNGGYIPGVRPGQATSNYLHSAMTRITLAGAIFL
                     TVIAVIPIVLTTHMNVPPAVGQYFGGTSMLILVGVLLDTMRQMESHLMMRHYDGFLKG
                     GRSGGSRLRGRF"
     gene            complement(5716..>5988)
                     /locus_tag="VG754_07490"
     CDS             complement(5716..>5988)
                     /locus_tag="VG754_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HWF19093.1"
                     /translation="NARHTVEFIPVNLDVLNSFKDGDKVDEAALRSAGLANGRLTRIK
                     ILGTGELTRKLTVSAHAFSASARSKIEAKGGTCEVIVRKPAQPANA"
BASE COUNT         1257 a         1706 c         1607 g         1418 t
ORIGIN      
        1 gaaacgtcca cccattccaa gatcgccagt tgggaagcat cgccctggcg ttcgcccaat
       61 ctcacgatgc gggtgtaacc gccatgacga tctttctgca ccggtgcgat gtcgttgaac
      121 agtatcttga ccgcgtcttc ctgatgcaaa cgcgcggctg cgagacgccg gtcatggacg
      181 gtgccgctct tgccgagggt gaccatcttt tcggcgaccg aacgggccgc cttggccttg
      241 gccaaggtgg tggtcacgcg cttgtgtttg atgaggctgc aaaccaaatt ggccagcatc
      301 gcgttccggt gtgtgccggt gcggccaagt ttggcggttc gcttgaggtg tcgcatatcg
      361 ttcgcttact ccacggtggt ggtcacgggc gcgccggttt cttccttggg cgattcgagc
      421 gtttcggcgt cgaatgtcat gcccaaagta agaccgagcg tggacaactt ttctttgatt
      481 tcgttgagag acttcttgcc gaagttgcgg tacttgagca tttcggcttc gctcttcatg
      541 gccaattgtc cgactgtggt gatgttggca ttgttcaagc agttggcggc gcggacgctc
      601 aactcaatct cattgacgct catgccgagc agtttcttga gcttggtctt ctcttcgtcc
      661 tgcttgtcca ccacttcctc gaattcaacc gcgttcttgt catagccgac gaacacgtcg
      721 agatgatgct gcaagatcgc cgaagcctgc gtgagcgcgt cgtcgggaga aatgcggccg
      781 tccgtccaaa gctcgatgat caaacggtca taatcggtgc gttgtccaac acgggcgctt
      841 tccaccgcgt accgaacgcg cgtgaccggc gaaaacaggg agtcaatggc gatcacgccg
      901 atggcctgat tggccttttt gttttcatcg cccgggcaga aaccgcggcc gaccttgact
      961 tcgagttcca tctcgaattt ctttttcttg tcgagcgtgc agatgtgttg cttgggattc
     1021 accaactcca cgttctggtt caactcgatg tcgctcgcga gcacttcgcc ttccttgttg
     1081 acggaaagca ggagtgtctg cggttcgcgg ctgtgggcct ttaacaatat cttcttcaaa
     1141 ttcaggatga tgtcggtgac atcttccaca acaccgtcaa cggtggtgaa ttcgtgcatc
     1201 gcgccatcaa tcttgatgga ggtgatggcc gccccttcca gcgaggaaag gagcacgcgc
     1261 cgaatggaat tgccgatggt atggccataa ccggtttcaa agggttcggc cacaaacttg
     1321 gcgtaggttt cagtggcggt tgactcttcc ttggtcaacc gtttgggcat ttcgaaacgt
     1381 cctaaacgta ctggcatttt ctttctttct ggcaaatgta atctgaccat cccggttact
     1441 tttcccgctg ccggaacggt ccgtttattt gcctttgtgt tctcccgttt gggagaatgg
     1501 gttcaattag cgggaataaa attcgacaac ggcctgctca ttggcaatgg gctggatttc
     1561 atcgcgggtc ggaatgcgca tgatgacgcc tttgaagcct tccttgttca acgaaagcca
     1621 atccggcacc gcgcggctcg tggccagttc cagattcttg gtggcaagct ggcgcgagac
     1681 gttggaatcc ttgatttcga ccacgtcgtt caccttgagt gcgaacgaag ggatgttcac
     1741 cttgcggcca ttcactttca catgcccatg gctgatcaac tgccgggcgg cggaacgtgt
     1801 ggtcgcaaag ccgagatgaa aagccacgtt gtccaggcgt gtctccaaga tctggagcat
     1861 ctgctcgccg gtgacaccac ggcgcttcaa cgcgcgttca taaacgccgc gaaactggcg
     1921 ctccatcaag ccgtaataat aacgaagctt ttgcttctcg atcaaaccga gaccgtaatc
     1981 ggtgtgcttg cggcgggact tggggccgtg cacgccggga ccgtaattgc ggcgctccag
     2041 atatttggtg gggccaaaga tgggcgtgcc gaaacggcgg ctgatgcgaa cgcggggacc
     2101 tgtataacgt gccataaatt agaatttcaa atttgaaatt tgatatttga gattgaactt
     2161 gattagacgc ggcgcttctt gcgtggacgg caaccattat gcggaatcgg cgtcacgtcg
     2221 cggatcatgg tgacttccaa accgatcgct tgcaacgcgc ggatggcgga ttcgcgtccg
     2281 gaaccggggc ctttgacgcg cacttcaact tcgcgcattc catgggccat ggcctgacga
     2341 gcggcatcct gggcgacttg ttgcgcagca taagcggtgc tcttgcgcga gcctttgaaa
     2401 ccaacgcgtc cggcgctgga ccagccgagc agattgccgt gaacatcggt gatgctcacc
     2461 tgggtgttgt tgaaagtggc aagaatgttg gcaatgcccg tggaaatgtt tttcgcgccc
     2521 ttggccttga tgattttctt tgcgttcgga tcatcggcca gcaactcggc agcagtcggc
     2581 gcagcggcgg caacaggagc gccttcggca ggcttgggtg cggcggcgtc tgcgccttcg
     2641 gcagcgggag cagcggcttt cttcggcttg gcgccttcag caggagcggc ctttgcttca
     2701 gccgcgcctt caggtttggc ttccttcttg ggagcgggtt tcttttttgt ttcgtcagcc
     2761 ataagaaata aagtttagtg gatgccggtc ttggcgttcg gattgcgttg cacaccgacc
     2821 gtcttgcgcg ggcctttgcg ggtgcgggcg ttggtctgcg tgcgttgtcc acgcacaggc
     2881 aggccgcgca ggtgacggac accgcgatag cacttgatgc cctgcagacg cttgaggttc
     2941 atgcccaatt cgcggcggag atcgccttcg atgacatatt tgccatcttg aatcgcgtga
     3001 acgatctgcg acatctgctg ctcgttcaag tccttcgcgc ggatgctcgg atcgatcttc
     3061 gctttttcga ggatgacttt cgagttgacc gggccgatgc cgtaaatgta gcgcaatgcg
     3121 atgtcaatgc gcttctcgcc gggaatttca acgccaatga tacgtgccat aaatttttag
     3181 ccttgccgct gtttgtgacg cgcattcgtg caaatcacac ggatgacgcc cttgcgcttg
     3241 accactttgc aattttcgca aagctttttt actgatgcgc gaactttcat atttgttttc
     3301 ctgtttcaat aatgcaaccg ttcgacaaat cgaacagcga catttccaat tttactttgt
     3361 cgccgggctt taacggcgcg ggattcgccc gggccttgcc cttaaaatga gccagcaccc
     3421 gatgtccgtt ggccagctcg accttgaaca tcgcgtgcgg aagcgcttca atcactgcgc
     3481 cttcaacttc gatggcatcc gttcgggcca cgtcaaaatc tccggttccg cttccgtgac
     3541 caagaccgta tgctcaaaat gggcggacag tgaaccatct tgcgtcacca ctgtccagcc
     3601 atcatttaag attttcacac cgggctgccc ggcattgacc atcggctcta tcgccagggt
     3661 cattccgggc cgcagcttct ggttgttctt cggatccaca aagttcggta tctgcggctc
     3721 ttcatgcatg gaccgcccaa caccgtgacc gacgaactcc cgaactatgc tgaacccatt
     3781 gccttccaca tagttctgaa tggcgcggga aatatccgtc acgcgatttc ccggaattgc
     3841 ctgcgcaatt ccctcataca gggccttttc cgtcacgtcc atcaaccgtt gcgcagcaac
     3901 gctgcaaccg cccacagcca ccgtccgggc cgtgtcaccg ataaaccggt tgtaaaccac
     3961 gcccacgtcg aggcttataa tgtcgccaaa ttccacccgc cgctgatttg ccaatccgtg
     4021 aacgacttgt tcattcaccg aaatgcaaat attgcacgga tacttccgat aacccaaaaa
     4081 cgcgctcttt gcaccgtaat gcttgatgcg agatgcggca aacaaatcaa tgtccttcgt
     4141 cgtcatgccc ggctttacga aggccgcgat ctcatccaac accgtgccag ccaccatgca
     4201 cgcgggccgc atcgcttcca agtcccgttc atttttcaaa acaatcattt tctgcccgct
     4261 ttagcgataa gggacttaat ctaagctaat tcggccaatg ttcaaaatct gccgcgcaac
     4321 cgcgaaccgc cgctccggcc gcctttaagg aagccgtcat aatggcgcat catcaggtgc
     4381 gattccatct ggcgcatcgt gtccagcaac acaccgacca aaatcaacat gctggtgccg
     4441 ccaaagtatt gcccgaccgc gggcggcacg ttcatgtgcg tcgtcaaaac aatcgggatg
     4501 acagcgatga cggtcaaaaa gatcgcgccc gcgagcgtga tgcgtgtcat ggcgctatgc
     4561 agatagttgc tggtcgcctg tcccgggcgc acacccggaa tatagccgcc gttcttcttc
     4621 aaatcatcgg cgatctgcaa ttcattgaac tgcgtcgccg tccagaagta agagaagaaa
     4681 agaatcatca atgtataaac caccaggtaa gtcatcgcgc cataattcag cgcattacca
     4741 atcttcccca agaacgcgaa actcgggaaa ttgcttccga tcatcgtgaa aagcttttgc
     4801 ggcaccatca ggatggcctg ggcgaagatg atcggcatga cacccgcgaa attcagccgc
     4861 agcggaagga acgaagtgcc gccggaataa actttgcggc ccacggcgcg ctgcgcatat
     4921 tgcaccggaa tttttctctg cgcctgcgtc actgcgatca cgcctgcaac caccgctatg
     4981 agcaacgcca ccaaaacaat gccgttgccg aaattgcctt tgcctgccgg tgaaaaatac
     5041 atgtccgcca atccttggaa agccgcgggc aaccgcgcca ggatgccgat ggtgatgacc
     5101 agcgaaatgc cgtttccaat cccccgttcc gtgatctgtt cgcccagcca catcagcagc
     5161 aaagtgccag tagttaaaat caagaccgtt tcaatgcggt accaccaaat gttgtcggaa
     5221 agcaccagag ggccctggtt ccagccgccg aaaatcgttt gcggattttc ccaactgtag
     5281 gccatgaaat atccctgccc gatgcacagc agcacggtca ggtagcggct gtatttgatg
     5341 atcttgaccc ggccgccttc ttcacgcgcc agcttgctca aagtcggcac cacggctgtg
     5401 agcaattgca tcacaatggt cgcactgatg taaggcatga ttcccaatgc cccaacagcg
     5461 caattctcca acgcgccacc ggtgaacagg ctgtacatgt tcagcaaacc accactgcca
     5521 gccgcgtggc tggccaggta agccgacagc gctgctccat caatgcttgg gatgcggatg
     5581 aacgccgcca aacggcaaat cgccaacaac atgaaggtga acccgatgcg ggacttcaac
     5641 tccggtatct tgaagcaatt actgaacgtg ttgacgatgg aagcaagcat ggctcaatga
     5701 tggaacaata tcattctagg cattggcagg ttgcgcgggc ttccggacaa tgacttcaca
     5761 cgttccgccc ttggcttcga ttttcgaacg ggctgaagcg ctgaacgcgt gcgcgctgac
     5821 ggtgagcttg cgggtcaatt caccagtacc aagaatcttg atgcgggtca gcctgccatt
     5881 ggcaaggccg gcactgcgca atgccgcctc gtccactttg tcaccgtcct taaaagagtt
     5941 caagacgtcg agattcaccg ggatgaattc cacagtgtgg cgggcgtt
//
LOCUS       DASZMM010000310         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2875716, whole genome shotgun sequence.
ACCESSION   DASZMM010000310 DASZMM010000000
VERSION     DASZMM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2875716"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VG754_07495"
     CDS             complement(<1..1345)
                     /locus_tag="VG754_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HWF19094.1"
                     /translation="MKPNKSPGGPGKMPPQWIAVLWYLPVMFFLLWMWQQAFSNLAIR
                     SIPYSEFKNHLEQGEVSEVTVKPDTVEGKIEPHAVASTHETGTNAAKAQTPEKPFLFR
                     TVRVDDSDLEEQLESAHVKFSGTRPGFFSEFLFAWLIPIGLMFLLWTFISRRMGGGAG
                     QSILSFGKSRARLVAEKETGVTFNDVAGCDEAKYELQEVVDFLKNPDRYKSLGAKIPK
                     GVLLVGPPGTGKTLLAKAVAGEAQVPFFQLSGSDFVEMFVGVGAARVRDLFVQAKVQA
                     PCIVFIDELDAIGRERGVHVGPVNDEREQTLNQLLVEMDGFAPNLGVILLAATNRPEV
                     LDRALLRPGRFDRQVVVDAPDIDGREAILRVHTRLVPLGSDVDLRRIARSTPGFSGAD
                     LANAVNEAALLAARRHASQIHQIDMEEAVEKVVAGPERKSRRLTDETKRRVAYHEV"
     gene            complement(1509..1694)
                     /locus_tag="VG754_07500"
     CDS             complement(1509..1694)
                     /locus_tag="VG754_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19095.1"
                     /translation="MTTLIILLLLGTCGYVHWLTGQYEAEANWQGQLQYPVTLEGNVA
                     LTNWTHRPTTPRQSPAM"
     gene            complement(1739..>2202)
                     /locus_tag="VG754_07505"
     CDS             complement(1739..>2202)
                     /locus_tag="VG754_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19096.1"
                     /translation="EGADREPVYQGRALSDWIDQYYWHLEASNSRVAPSMKPVWATNR
                     SEAMAAVKQMGTNALPALFDMLRYGNSRRVRWGEWSYGVWYRTAWLDWVPRFITRRYF
                     LPPGVAEIKHEYGQLGLEMLGPIAKPALQKALTEPNIRVRETATNLLAKMP"
BASE COUNT          478 a          690 c          551 g          483 t
ORIGIN      
        1 ccacttcatg ataggcaaca cggcgtttcg tttcgtcggt caaccgccgg ctcttgcgtt
       61 ccggcccggc gacaactttt tccaccgctt cctccatgtc aatctgatgg atttgcgacg
      121 catgacgccg cgccgcgagc aacgccgcct cgttcaccgc gttcgcaagg tccgcgccgg
      181 aaaaaccggg cgtggaccgc gcaatccttc gcaaatccac atccgaaccc aaagggacca
      241 gccgcgtatg cacgcgcaaa atcgcttcgc gcccgtcaat gtccggcgca tccacgacca
      301 cttgccggtc gaagcgtccc ggacgcagca acgcgcggtc gagcacttcg ggccggttcg
      361 tcgccgccag caaaatcacg ccaaggttcg gcgcaaaacc gtccatctcc accagcaatt
      421 gattcagcgt ctgttcccgt tcatcattca ccggcccaac atgcacgccg cgttcgcgcc
      481 cgatggcatc caattcgtca atgaaaacga tgcacggcgc ctgcaccttg gcctgcacaa
      541 acagatcgcg cacacgcgct gcgcccacgc ccacgaacat ttccacgaaa tcacttccgc
      601 tcaattgaaa gaacggcacc tgcgcctcgc ccgcgacggc ttttgccagc agcgtttttc
      661 ctgtcccggg cggcccgacg agcagcaccc cctttggaat ttttgcgccg aggcttttat
      721 aacggtccgg gttttttaga aaatcgacca cttcctgcaa ttcatatttc gcctcgtcgc
      781 aaccggccac gtcattgaac gtgacaccag tttctttctc agccaccagg cgcgcgcggc
      841 tcttgccaaa actgaggatg gactgccccg ctccgccgcc catgcgccgg ctgatgaaag
      901 tccacagcag gaacatcagg ccgatgggga tcagccacgc aaaaagaaat tccgaaaaga
      961 aaccgggacg cgtcccgctg aactttacat gcgccgattc gagttgctcc tccaaatcgg
     1021 aatcatccac gcgcaccgtg cggaacagaa atggtttctc aggcgtctgc gccttggcgg
     1081 cgttggtgcc ggtttcgtgg gttgaagcga cggcatgcgg ttcgattttt ccctcaacgg
     1141 tgtccggctt gaccgtcact tcggaaactt cgccctgttc caggtgattt ttgaattcac
     1201 tgtaaggaat cgagcgaatg gcgagattcg aaaatgcttg ttgccacatc cagagaagga
     1261 aaaacatgac cggcagatac cacaatacgg caatccattg cggcggcatc ttgcccggtc
     1321 cgcctggcga tttgttcggt ttcatgaaaa ttagcgcctt cctcccatcc cggccggtcg
     1381 cttcgaacca gtctcatccc tccttattag agcgattcac cataaattat aaatacatca
     1441 ccccataatg taacaccatg gggagaaacc gcatcccgga tgcggaaggg catggcccaa
     1501 cgatgcaatc acatggccgg ggattggcgg ggagtggttg gcctgtgtgt ccagttggtc
     1561 aatgcaacat tgccttccag agttaccggg tattgcaatt ggccctgcca gttggcttcc
     1621 gcctcatact gtccggtcaa ccaatggaca taaccgcacg ttccaagcag cagcagaatg
     1681 atcagggtcg tcatggccac atgcaacctt cttaatggca tcgttttcat tttacagttc
     1741 atggcatttt agcgagtaga tttgtggctg tttctcgaac gcgaatgttg ggttctgtga
     1801 gtgccttttg taatgctggc tttgcaatcg gccccaacat ttccaatccc aactggccgt
     1861 attcatgttt tatttcagcc acgccgggag ggagaaagta gcgccgtgtg atgaaacgcg
     1921 gcacccaatc aagccatgca gtccgatacc acactccgta ggaccattca ccccacctga
     1981 cgcgccgcga attcccatag cgcagcatgt caaacaaagc gggcagcgcg ttagtgccca
     2041 tttgtttaac tgccgccatt gcctcagaac ggttcgtggc ccatacgggc ttcatcgacg
     2101 gcgcgactcg cgagttgctt gcttccagat gccaatagta ttgatcaatc cagtcactca
     2161 aagcgcggcc ttgataaacc ggctcgcggt ctgccccttc cg
//
LOCUS       DASZMM010000311         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3978308, whole genome shotgun sequence.
ACCESSION   DASZMM010000311 DASZMM010000000
VERSION     DASZMM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3978308"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VG754_07510"
     CDS             <1..1276
                     /locus_tag="VG754_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWF19097.1"
                     /translation="HIESDGRGGFGSSLIFEEMGLRYLGPIDGHDLPLLMNSLEFAKS
                     CDQPVVLHVLTKKGRGYDVAIQQPEKFHGTGPYDIQTGVSPSPKPGTAPAWQDVFGQT
                     MVKLCQKDNYVVGITAAMPSGTGLKALEKAMPQRYYDVGIAEEHAVLFAAGMATMGFH
                     PVVAIYSTFLQRSYDCIIHDVALQNLPVIFCMDRAGLSPQDGPTHHGLFDIAYLRCVP
                     NIVAMAPKDEDELADMMFTATHLKQPAFIRYPRGAAEGVPLKEQPKIIEVGKAEVIQN
                     FAHTKGRKVTLFGLGNMLSVARQAAKELMAEGFDVAIINPRFTKPLDAGTTELFGRAA
                     DVVVTLEDHVLPGGYGSSVLELYSEKQVQTPVVRIGWPDKFIEHATTVEYLREKHGLT
                     VANTVAQVKALLNGEAHANVSTADTTTLIGVA"
     gene            1315..1575
                     /locus_tag="VG754_07515"
     CDS             1315..1575
                     /locus_tag="VG754_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HWF19098.1"
                     /translation="MNVNLTAEQEKFIAEKVRSSHYSSPEGVVSEGLRLVQAKEEYER
                     HLAELRREIDIGLEQIRRGEVVDGREAIARLLEKNRMRPRSE"
     gene            complement(1668..>1980)
                     /locus_tag="VG754_07520"
     CDS             complement(1668..>1980)
                     /locus_tag="VG754_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19099.1"
                     /translation="ILPPAGGVMSLYAISQFEPIVRFYQKGECAGTQGKKCRARLLEE
                     TWHITRPMNHSDQLHAIVQRFIKYQIVSDRKPTQSPAQIRTVIAEFRMLGKQLASFKN
                     A"
BASE COUNT          445 a          489 c          615 g          431 t
ORIGIN      
        1 ccatatcgaa tcggacggcc gcggcggttt cggcagcagt ttgattttcg aggagatggg
       61 gctgcgttat ctcgggccga ttgacgggca tgatttgccg ttgctgatga attcgctgga
      121 gttcgccaag agttgcgacc agccggttgt tttgcatgtg ctcaccaaga agggcagggg
      181 gtacgacgtg gcaatccagc agccggaaaa attccacggc accggcccgt atgacatcca
      241 aaccggcgtt tcgccgtcgc cgaaacccgg caccgcgccc gcgtggcagg atgttttcgg
      301 ccagacgatg gtcaagcttt gccagaagga caattacgtc gtgggcatca ccgcggcgat
      361 gccgagcggc acgggattga aggcgttgga aaaggcgatg ccgcaacgct attacgatgt
      421 gggtatcgcc gaggaacatg cggtgctgtt cgccgccggg atggcgacga tgggttttca
      481 tccggtcgtg gcgatttact cgacgttcct gcaacgctcc tacgactgca tcattcacga
      541 tgtcgcgttg caaaatctgc cggtgatttt ctgcatggac cgcgccggac tttcgccgca
      601 ggacggcccg acgcatcatg ggttgttcga tatcgcgtat ttgcgttgcg tgccaaacat
      661 cgtcgccatg gcgccgaagg atgaagacga acttgcggac atgatgttca ccgcaacgca
      721 tttgaagcag ccggcgttca tccgttatcc gcgcggcgcg gcggaaggcg tgccattgaa
      781 ggagcagccg aaaatcatcg aggtcggcaa ggccgaggtg atccagaatt tcgcgcacac
      841 caaggggcgc aaggtgacgt tgttcggcct gggcaacatg ctcagtgtcg cgcggcaggc
      901 ggcgaaggaa ttgatggccg aaggctttga cgtggccatc atcaatccgc gtttcacaaa
      961 accgctggac gcgggcacga cggaattgtt cgggcgcgcg gcggatgtcg tggtgacgtt
     1021 ggaagaccat gttttgcccg gtggttacgg cagcagcgtg ctggaattat acagcgagaa
     1081 gcaggtgcaa acgccggtgg tgcgcatcgg ctggccggac aaattcatcg agcacgcgac
     1141 cacggtggaa tatctgcgcg agaagcacgg cttgaccgtt gcgaacacgg tggcgcaggt
     1201 gaaggcgttg ctcaacggag aagcgcacgc gaacgtgagc acggcagaca cgacgacact
     1261 gattggggtc gcgtgatgaa cttgtgacat gctcaacgta taaatcgtat taccatgaat
     1321 gttaatttga cagctgagca agagaaattc atcgcggaga aagttcggtc gagccattat
     1381 tcatcccccg agggagtcgt cagtgaaggt ttgaggttgg tacaggcgaa ggaagaatat
     1441 gagcgccatc ttgccgagtt gcgtcgggaa attgatatcg gattggagca aatccggcgc
     1501 ggtgaagtcg ttgatggcag agaggctatt gcccggctgc ttgaaaaaaa ccggatgcgc
     1561 cctcgttccg aatgagggtt gaatattcga aggaagccat ccgagatttg gaacaaatcg
     1621 aggagtacat atcgcaggat aatcctgacg ctgctgccaa gctgatttca agcattcttg
     1681 aaacttgcga gttgcttgcc cagcatccta aactcggcga tgaccgtgcg tatttgcgca
     1741 ggggactgcg tgggtttccg gtcggaaact atttgatatt ttatgaaccg ttggacaatg
     1801 gcatgcagtt ggtccgagtg attcatgggt cgcgtgatgt gccaagtttc ttccaataac
     1861 ctagcacgac atttctttcc ttgcgttccc gcgcattcac ctttttgata gaaacgaacg
     1921 attggttcaa attgacttat ggcatataaa gacatcactc cgcccgccgg cggcaagatt
//
LOCUS       DASZMM010000312         5899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2043332, whole genome shotgun sequence.
ACCESSION   DASZMM010000312 DASZMM010000000
VERSION     DASZMM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043332"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..895
                     /locus_tag="VG754_07525"
     CDS             266..895
                     /locus_tag="VG754_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF19100.1"
                     /translation="MESAKKQLLRFPGEYNSGGAHEQEASEDRRLLAVICDGNQQALA
                     VLYQRRGALLYSMLVRMLGNEMEAQEILQDTFLLIWRRAAEYHAERSSALAWMVMIAR
                     GRGWDRLRARARRSINQAAYESEMASLEVEINIPPQAEKDELVSACASALNELPEEQG
                     RALQLAFFRGWTHEEIAGASGEPLGTIKARIRRGLLALRKALKDYHARP"
     gene            882..2072
                     /locus_tag="VG754_07530"
     CDS             882..2072
                     /locus_tag="VG754_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19101.1"
                     /translation="MPDHDPLGDEAAQYVLDGLSQAERAGFETRMAHSAELRAQVREL
                     EDGMAALALAAPRRKPPAKLWGQIEKELARESGWRKAVCELRHDWWRNGWAAAAACLV
                     GWALYAFYVNEMRPSTRAPMEISEASATQRGTSTNSAPMEKNAAIHPPSRTAPTNLAR
                     SDAALVQVQSLRKEIADLKTQVAQMSLSLAQQQAMLGESNRLKFLKLTPASADDSTTI
                     SQLSPALQRAIFIAMARELGWLPPENPPTSEESGVVRLPSTNQWNVDFVDLRPGANNP
                     SSNPLSPKTESPLVSSAPASAPPTAASPTIPAFISGDNLIMAIDSTTVAKGSSLTFST
                     GATPDTQQIQGTTTLGDNPMVVTMPINDYIANQPNGVTVNIISTSTSGSSVLQFNVPP
                     TINH"
     gene            2079..3479
                     /locus_tag="VG754_07535"
     CDS             2079..3479
                     /locus_tag="VG754_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19102.1"
                     /translation="MKKSLIVQFTILFMSLAILPARAQFCYFTILAGQTNAIGTNDGT
                     STAALFNHPRGLAVDPSGNVYVADTYNSSIRRITPQGTVTTLTFESLSGVIANVVTTN
                     QPWPPIVYEPFSFTAPQTLLFDTNSNLYISDNNVIYETLLSPDTNTSDPGQFLVTAFA
                     GALDPTPPFTGQTGLQFNHPCGMAFDTNGNLYLADQYNQVVREISPAASVTTLAGMLG
                     NPDFENGAGTNAAFDFPYGIAVGQSNIVYVSDSVAGIRQIIQSDFVTNFFWKGGYTIP
                     RAIATDASGNVYAAVASYGTNYVLRIAPDGTNMIVLGSLPTTGPISAIALDANTNIYL
                     ADQKNSVIIKSTRYPQFFNGQASLTNGNFFLAFTNGMPFGYYDLGSFNFPIFYHYDMG
                     FELFMDAGDGQGSAYLYDYASSTYWYTSPQLFPYLYDFSLNTWLYYFPDKNQPLHYSS
                     NPRYFFNFAKGVVITK"
     gene            complement(3543..4874)
                     /locus_tag="VG754_07540"
     CDS             complement(3543..4874)
                     /locus_tag="VG754_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19103.1"
                     /translation="MKRQQQFSRRSFLKTLGTAAFAAPFITRNLMAQSPNGVVRHASF
                     GTGGQAWSDLTQLSNVPRFKVVAVCDVDLDRTTEARKKFPDARVYQDWRELLAKEAKH
                     IDSVNVSVPDHMHAPIAVSAMRLGKNVYGQKPLAHDLYEVRRMTEVARREKVVTQMGI
                     QIHSSKVYRTGVQIIRNGTIGKIKEVHSWCSKSWGDPSPKPERADPVPPNLNWDLWLG
                     VCAERPFIGDKYYHPMNWRKRLDFGTGTFGDMGCHIFDPVFKSLELTAPISVRSEGAA
                     PNEWNWALDSKIHYTFPGTKFTAGTTLPVTWYDGTSRPPVEIIKLMESDELPAIGSMF
                     IGTEGVMVLPHIARAQLYPNAKFENFKYPEVAEVSHWASYIDACLGEGKTSANFDYAG
                     PLTEAVLLGGVATHFPLTTLEWDAHKLKFSLAEANHFVRRSYRKGWHVRGL"
     gene            complement(4871..5290)
                     /locus_tag="VG754_07545"
     CDS             complement(4871..5290)
                     /locus_tag="VG754_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF19104.1"
                     /translation="MTSRAGCRLLHLARFMAMYYASKAFVYSFSCALAEELKESGGSV
                     TTLCPGMTHSEFHARANLKRSAALPMMSAEVMPKNRLSHHDERQANRRRKHRENDADT
                     PGHSDRGKNKSVRGNNEEAFRTFKLDGCMAHAEHFRA"
     gene            5386..>5899
                     /locus_tag="VG754_07550"
     CDS             5386..>5899
                     /locus_tag="VG754_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19105.1"
                     /translation="MNKINLQIIKRKLFTALVSAAIGFVLAGFSGIVQAQNAPVSLPP
                     GVQDVLKLSQAGVGEDVIMAQVKNTGATYNLNADQLIYLTKSGVSQNVIRALITGNTG
                     GAPVAAPAPVPTTEPSSLPPPPTTVPAAPSVETAPQVPGAIPAPQPIVVPSQGANFDS
                     FHDQLAPYGTW"
BASE COUNT         1304 a         1733 c         1539 g         1323 t
ORIGIN      
        1 cgggcaccaa cggcctcttc caatacatcg atctgtctgc gaccaattac ccgatgcgat
       61 attaccgcac ggcagtgccg ccgggattct gagccgttga tgaagattgc cagagccgtt
      121 gcgggtgcaa gcctgcaacg gctcttgtat ttttgcggca cgaccgtgtc gcaaaaggca
      181 tttgtcgttc cttctgcaaa atttttgcgt ccaaactctc ccgggcggcg gatatactcc
      241 cgaagcgaat tgaaaatcgt taacggtgga atcagccaaa aaacaattgc tgcgtttccc
      301 gggtgagtat aattccggcg gcgcgcatga acaggaagcc agtgaggaca ggcgcctgct
      361 cgccgtcatt tgcgatggaa atcagcaggc gcttgccgtg ctctatcaac gccggggcgc
      421 gctgctttat tcaatgctgg tccgcatgct tggcaacgaa atggaagccc aggaaatcct
      481 gcaagacacg ttcctgctca tctggcgccg ggccgccgaa tatcatgctg aacgctcctc
      541 ggcgctggcc tggatggtca tgatcgcccg cggacgggga tgggaccggc ttcgcgcgcg
      601 cgcgcgacgg agcatcaatc aagccgctta cgaaagcgaa atggcgtcct tggaagtgga
      661 aatcaacatc ccgccacagg ccgagaagga tgaactcgta tccgcttgcg cctcggcatt
      721 gaatgagttg cctgaagaac aggggcgcgc tctgcaactc gctttttttc gcggctggac
      781 gcacgaagaa attgccggcg cgagcggcga accgttggga accatcaaag cgcggattcg
      841 acgggggctg ctcgccctgc gcaaagcgct gaaagattac catgccagac catgatccac
      901 taggtgatga agccgcccag tacgtgctgg acggcctgag ccaggccgag cgcgccgggt
      961 ttgaaacgcg catggcgcac tccgccgaac tccgcgcaca ggtgcgcgag ttggaggacg
     1021 gaatggccgc gcttgcgctc gccgcgccgc gccgcaaacc tccagcaaaa ctttggggcc
     1081 agattgaaaa agaactggcc cgcgaatccg gctggcgcaa ggccgtttgc gagctgcgtc
     1141 atgattggtg gcgcaatggt tgggcggctg ccgcagcctg tcttgtcgga tgggcactct
     1201 acgcttttta tgtgaacgag atgcgtccga gcacccgggc accaatggag atttccgaag
     1261 cgtcggccac gcaacgcggc acgtccacaa attctgcgcc gatggaaaaa aatgccgcca
     1321 ttcacccgcc gtcacgaacc gccccgacga atctcgcgcg ttcggacgcc gcccttgtgc
     1381 aggtacaatc gttacgcaaa gagatcgcgg atttgaagac gcaggtcgcg caaatgtcgc
     1441 tctcccttgc acagcaacaa gcgatgctcg gcgaatccaa ccgcttgaaa ttcttgaaat
     1501 tgacaccggc gtcagccgat gactcaacaa ccatcagcca gctttcaccc gccctgcaac
     1561 gcgcgatttt catcgccatg gcgcgtgaat tgggctggct gccacccgag aatccgccca
     1621 cgagcgagga gagcggagtc gttcgcctgc cttccaccaa ccaatggaac gttgatttcg
     1681 ttgacttgcg tcccggcgcg aacaatccat cctcaaatcc gctttcgccg aaaacagaat
     1741 caccacttgt ttcatccgca cccgcttccg ctccgccaac ggctgcatcg cccaccattc
     1801 cggcattcat ttccggtgac aacctgataa tggcgattga ctccaccact gtggccaaag
     1861 gaagctccct gacgttctcc actggagcca cacccgatac gcagcaaatt cagggcacca
     1921 cgactcttgg agacaacccc atggttgtca ccatgcccat aaatgattat atcgcgaacc
     1981 aaccgaatgg agtgacagtg aacatcattt ccacatctac ttccggttcg agtgtccttc
     2041 aatttaatgt cccaccaacc atcaaccatt aaaacattat gaaaaaaagc ttgattgtcc
     2101 aattcaccat cctgttcatg tcactcgcca ttctgccagc ccgcgcgcag ttttgttatt
     2161 ttaccattct cgcgggacag acaaatgcca tcggcacgaa tgacggcacc agcactgcgg
     2221 cgctgttcaa tcatccgcgc ggtcttgctg tggacccttc gggaaatgtt tacgtggctg
     2281 atacgtataa ttcctccatc cgccgcatca cgccacaggg caccgtcacc accctgacgt
     2341 tcgaaagcct gtcgggcgtt atagccaatg tcgtcacaac gaatcagccg tggccaccaa
     2401 tcgtttatga accattcagc ttcacagctc cccaaaccct cctgttcgac accaactcaa
     2461 acctttacat cagcgacaac aacgtcattt acgaaacatt actttctccg gataccaata
     2521 ccagcgatcc tgggcaattt ctggtgactg cttttgccgg ggcgctggat cccacgccgc
     2581 cttttaccgg gcagaccggg cttcaattca accatccttg cggcatggcg tttgacacaa
     2641 atggcaatct ctatctggcc gaccaataca accaagtagt acgtgaaatc tctcctgcag
     2701 catcagtgac aaccctggca ggcatgctgg gaaatcccga tttcgaaaat ggcgcgggca
     2761 cgaacgcggc atttgacttt ccttacggca tcgccgttgg ccaatccaat atcgtttatg
     2821 tcagcgacag cgttgcgggc atacgccaga tcatccagtc cgatttcgtc accaactttt
     2881 tttggaaggg cggctatacg attccccggg ccattgccac agatgcgtcg ggaaatgttt
     2941 acgccgccgt cgcgtcgtat gggaccaact atgtgctgag aatcgccccg gatggcacca
     3001 acatgatcgt gcttgggtca ctgccgacaa caggccccat ctctgccatc gccttggatg
     3061 ccaacacaaa tatctacctc gccgatcaaa agaacagtgt catcatcaag agcacgcggt
     3121 atccgcaatt ctttaacgga caggcttcac tcaccaacgg caattttttt ctcgcgttca
     3181 ccaatggaat gccgttcggc tattacgatc ttggcagctt caattttccc atcttttatc
     3241 attatgacat ggggtttgag ctgttcatgg atgcgggaga cggccagggc agcgcttacc
     3301 tctatgatta tgccagttcc acttattggt acaccagccc gcaacttttc ccctatctct
     3361 acgatttttc actgaacaca tggttgtatt attttccgga taaaaaccag cccttgcatt
     3421 actcctccaa tccgcgttat ttcttcaact tcgccaaagg agtcgtcatc acgaagtagc
     3481 gaaaatgaaa cggcccgccg ttgcgtgctg ctcggattgg aaaattgcag gagcctgcct
     3541 tgctacaggc ccctgacgtg ccagcctttg cgatagctgc ggcgcacgaa atggttcgcc
     3601 tcggccaggc tgaatttgag cttgtgcgcg tcccactcca gcgtggtcag ggggaaatgc
     3661 gtcgccacgc cgccgagcaa aactgcttcg gtaagcggcc cggcatagtc gaaattcgcg
     3721 gaggtttttc cttcgccgag acacgcatca atatatgacg cccaatggct tacttccgca
     3781 acttcgggat atttaaaatt ctcgaatttg gcgttcggat acaactgcgc gcgcgcgatg
     3841 tgcggcagca ccatcacgcc ttccgtccca atgaacatgg atccaatggc tggcaattcg
     3901 tcgctttcca tcaatttgat gatttcgacg ggcggccttg acgtgccgtc ataccatgtc
     3961 accggaaggg tcgtgccggc agtgaacttc gtgccgggaa atgtgtaatg gatcttcgaa
     4021 tcgagcgccc aattccattc gttcggcgcg gcgccctctg aacgcacgct gatcggcgcg
     4081 gtcaattcca atgatttaaa aaccgggtcg aagatgtggc aacccatgtc cccgaaagtg
     4141 ccggtgccga aatcgagccg tttgcgccag ttcattggat gataatattt atccccaatg
     4201 aagggacgtt cggcgcagac gcccagccag aggtcccaat tgaggttcgg cggcacggga
     4261 tcggcgcgtt ccggcttggg cgaaggatcg ccccatgatt tcgagcacca tgaatgcacc
     4321 tccttgattt tgccgatggt gccgttgcgg atgatctgca ctccggtacg gtaaaccttc
     4381 gaggaatgga tctggatgcc catctgcgtg acgacttttt ccctgcgggc cacttcagtc
     4441 atgcgacgga cttcgtaaag atcgtgtgcg agcggtttct ggccgtaaac atttttacca
     4501 agccgcatcg cgctcacggc aatcggggcg tgcatgtggt ccggcacgga cacattcacg
     4561 gagtcaatgt gcttggcctc cttggcgagc aattcacgcc aatcctggta aacccgcgca
     4621 tcgggaaatt tcttgcgcgc ttccgtggtg cggtcgaggt ccacgtcaca gacggcgacc
     4681 accttgaatc gcggaacgtt gctgagctgg gtcagatcgc tccatgcctg tccgccggtg
     4741 ccgaagctgg catggcgcac cacgccgttg ggcgattgcg ccatcagatt gcgtgtgatg
     4801 aacggagcgg cgaaggctgc agtgccaagg gtcttgagga aagaacgacg tgaaaattgc
     4861 tgctggcgtt tcatgctcga aaatgctccg cgtgcgccat gcacccgtca agcttaaatg
     4921 tccggaatgc ctcttcgttg tttcctctca ccgatttatt tttgccgcga tcggagtgac
     4981 caggcgtgtc ggcatcattt tcgcgatgct tgcgacgacg attggcttgc ctttcatcat
     5041 ggtgcgataa ccgatttttc ggcattacct ccgcgctcat catgggcaat gcggcgctgc
     5101 gtttcaaatt cgcgcgcgcg tgaaattccg agtgcgtcat gccgggacaa agcgtggtga
     5161 cgctcccgcc gctttccttc aattcctccg ccagcgcgca ggaaaatgaa taaacgaacg
     5221 ccttgctcgc ataatacatt gccatgaacc gggccagatg caaaagtctg caacctgccc
     5281 ggctggtcac gggtctttga tagtcgttcg agggtgattt ttgaaatatt taccgctcct
     5341 taacgtcgaa tgggtgttaa attggtgaaa gaagctgaaa aaagtatgaa taagataaat
     5401 ttgcaaatca ttaaaagaaa gctgtttacg gcgcttgtat ctgccgcaat cgggtttgtt
     5461 ttggcgggtt ttagtggtat cgtccaagcg caaaacgcgc cggtcagttt gcccccaggc
     5521 gtgcaggacg tgctcaagtt gtcgcaggcc ggtgtgggcg aggatgtcat catggcccaa
     5581 gtcaagaata ccggcgcaac ttacaatttg aacgccgacc agctcattta tctgaccaaa
     5641 agcggcgtct cgcagaatgt gattcgggcg ttgatcaccg gcaataccgg cggagctcct
     5701 gtcgccgcgc ctgctccggt tccaacaacg gagccatcat cgcttccacc accgccaacc
     5761 actgttcctg ccgcgccatc ggttgaaaca gcaccgcaag ttccgggtgc gattcctgct
     5821 ccgcaaccga ttgtcgttcc atcgcaaggc gcgaacttcg acagttttca tgaccaactc
     5881 gctccttacg gcacttgga
//
LOCUS       DASZMM010000313         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_416990, whole genome shotgun sequence.
ACCESSION   DASZMM010000313 DASZMM010000000
VERSION     DASZMM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416990"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VG754_07555"
     CDS             complement(<1..776)
                     /locus_tag="VG754_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19106.1"
                     /translation="MDTKDSMSDGKLGTTRRDFLKKTTTIAAAVATTNLFKTPVYGQN
                     QAPSTGRTIGANDRINVAVIGIGVGIGQNHLKGINDNAAENNVAVVAASDLFSKRRDM
                     CKDLAGLKDNDIHLEWEKVIDRKDVDAVVIATHDPWHAPISIAAIQAGKHVYCEKPMS
                     RYLGEAFQVYDAVKASPKLAFQIGAQGTSAGGWNKCAELVNAGKIGTLVWSQGYYCRN
                     SKTGEWNTAPFNVDPASSAQTIDWDRWQQRVHNKIPFSAD"
     gene            complement(1040..1618)
                     /locus_tag="VG754_07560"
     CDS             complement(1040..1618)
                     /locus_tag="VG754_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWF19107.1"
                     /translation="MSLSEWESRYQSGDMPWEKGEASPGLVDFIAAHPELKRGTVCVP
                     GCGTGHDARVWARASFTVTGCDIAPSAIRLSRERTAAAGLQAQFLQSDFLRDKPPQHF
                     DWLFEHTLFCAIDPSERDLYVKALLDWLAPGGQYLAVNYLIPDKDGPPFGTTREELWQ
                     RFSPHFDLLQEWVPRSYPNRTGLELMLWWKRR"
     gene            complement(1625..>2095)
                     /locus_tag="VG754_07565"
     CDS             complement(1625..>2095)
                     /locus_tag="VG754_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF19108.1"
                     /translation="FSDRKLTDFRNRRVGFVFQAYHLLPELTALENVCLPGRIARLPV
                     GDVVKRARELLARVGLAGRVDHKPSELSGGEQQRVAIARALINAPELVLADEPTGNLD
                     SKTGTEIMDLLCTLRTEHQTTLVIATHDNKIAERAPKVVKLADGLIVENAKVEK"
BASE COUNT          423 a          587 c          614 g          471 t
ORIGIN      
        1 tgatcggcgc tgaacggaat tttattgtgg acgcgttgct gccagcggtc ccagtcaatt
       61 gtctgggcgg aagaagcggg gtcaacattg aacggagcag tgttccattc gccggtcttg
      121 ctgttgcggc aataatagcc ctggctccag acgagcgtgc cgatcttgcc ggcgttgacg
      181 agttcggcgc atttgttcca gccacccgcc gaggtgcctt gcgcgccgat ctggaaggcc
      241 agcttgggag acgccttcac tgcgtcatac acttggaacg cctcgccgag ataacggctc
      301 atcggcttct cgcaataaac atgcttgccg gcctggatgg ccgcgatgga aatgggcgcg
      361 tgccagggat cgtgcgtggc gatgacgacg gcgtccacgt ccttgcggtc gatcactttt
      421 tcccattcga ggtggatgtc attgtccttg aggccggcga ggtctttgca catgtcgcgg
      481 cgtttgctga aaaggtctga ggcggcgacc acggcgacgt tgttctcggc ggcattgtca
      541 ttgatgccct tgagatggtt ctggccgatg cccaccccaa tgccgatgac ggccacattg
      601 atgcggtcgt tcgcgccgat ggtgcggccg gtcgaaggcg cttgattttg gccgtaaacc
      661 ggggtcttga acaggttggt ggtggccacc gcggccgcga tggtcgtggt tttcttgaga
      721 aaatcccggc gtgttgtccc gagcttcccg tccgacattg agtcttttgt atccataaat
      781 cttataatcc agccatgagc agttggacga ttattaacgc aaatatgttc agggtcaacc
      841 cgcatctcca tgcggtttcg ggctaattcg ggaatttaaa ccatggattc aaaaatgaac
      901 acatcgaaca tccaacatcg aacgtcgaac gctgaatgga tgcaactcgc gccattggat
      961 gttcagcgtt cgatgttcag cgttctgcgt ttcatcctgt ccatgtccat cagcgtccac
     1021 ctgtggttga aaagaaattc taccgccgtt tccaccaaag catcaattcc aggccggtgc
     1081 gattgggata agagcgggga acccattcct gcaacaaatc gaaatgcggc gaaaaacgtt
     1141 gccacaattc ttcgcgcgtc gtgccgaacg gcgggccgtc cttgtcggga atcaaataat
     1201 tgaccgcgag atattgaccg cccggcgcaa gccagtcgag cagggccttc acataaagat
     1261 cgcgttcgga aggatcaatc gcgcaaaaaa gcgtgtgctc gaagagccag tcaaaatgtt
     1321 gcggcggctt gtcccgcaaa aaatcgcttt gaaggaactg cgcctgcaaa cccgctgccg
     1381 ctgtccgttc gcgcgacaat cggatggcgc tcggcgcgat gtcgcaacca gtcaccgtaa
     1441 aactggcccg cgcccaaacg cgcgcgtcat gtcccgttcc gcagcccggc acgcaaaccg
     1501 tcccgcgttt tagttcagga tgcgccgcga tgaaatccac caggcccggc gatgcctcgc
     1561 ccttttccca aggcatgtcg ccgctttgat aacgtgattc ccattcggat aatgacattg
     1621 cttgctattt ttctactttc gcgttctcca caatcaatcc atccgcgagc ttcactacct
     1681 tgggcgcgcg ttcggcgatt ttgttgtcgt gcgtggcgat gacgagggtg gtctgatgtt
     1741 cggtgcgcag ggtgcaaagc aaatccatga tttcggttcc ggtcttggaa tcgaggttgc
     1801 cggtcggttc gtcggcgagg accaattcgg gagcattgat gagcgcgcgg gcgatggcga
     1861 cgcgttgctg ttcgccgccg gaaagttcgg acggcttgtg atccacgcgt ccggcaaggc
     1921 cgacgcgtgc cagcaattcc ctggcgcgct tcacgacatc gccgacaggc aaccgtgcga
     1981 tgcgtccggg caggcagacg ttttccagcg cggtcagttc cggcaacaaa tgataggcct
     2041 ggaaaacaaa acccacgcgg cggttgcgga aatccgtcaa cttgcggtcg gagaa
//
LOCUS       DASZMM010000314         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5068197, whole genome shotgun sequence.
ACCESSION   DASZMM010000314 DASZMM010000000
VERSION     DASZMM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5068197"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VG754_07570"
     CDS             <1..922
                     /locus_tag="VG754_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19109.1"
                     /translation="PAAGINPSLEATITLDSIPPTSHVLPLPAQSQLLQVPVSWTGQD
                     NANGPGIAKYNVYVSDNGGSWTLWQSATTATNATFQGKPQHTYGFTSQAIDNAGLLEP
                     QHTTADTATLIVANPQFQFTVTPASANLATNASFNYNITVKNIGTLSLSNVIMSNAMP
                     AGISFDYVQYGRGSCDIEDTYLLWSLGNMNTNVSASMTVTATTGANGTWTNLFGVADS
                     QGAATAKAVQLLYVGITPPVLLNIALTNSQVLLSWPSTIGNFGLQTTTNLVSQTSWTA
                     ATNVPVTNGGTIIVALPVTRTNQFFRLKGQ"
     gene            1076..>1729
                     /locus_tag="VG754_07575"
     CDS             1076..>1729
                     /locus_tag="VG754_07575"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HWF19110.1"
                     /translation="MTFIDLFCGCGGFSLGLNRAGLTGLAAIDFNPEAIAVFKRNLPQ
                     IPHSLGKDLTGFAPAALAKLIGTAEVDVIVGGPPCQGFSTVRQVDGANHGKRLVDDAR
                     RHLYQEFLRYVNFFQPKVFVMENVLGIRSASEGEYFSRVQKEARALGYRVHAQVEDCM
                     ELGVPQKRRRQLFIGTRRDLPDYFRPELKPAPRIDVCRDGTQGLKVPITLWEAIGDLP
                     "
BASE COUNT          395 a          473 c          460 g          401 t
ORIGIN      
        1 ccccgctgcc ggtatcaacc caagcctgga ggcgaccatc acactcgatt ccattccgcc
       61 aacgagtcat gttttaccgc tgcctgcaca aagccaattg ttgcaagtcc cggtttcatg
      121 gaccggccaa gataatgcga acggccccgg cattgccaag tacaacgttt acgtttccga
      181 caacggcggt tcgtggacgc tctggcaatc tgccacaacc gccacgaatg cgacattcca
      241 aggcaagccg cagcacacct atggtttcac cagccaagcg attgacaacg ccggtttgct
      301 tgaaccgcag cacactacgg cggataccgc gacgttgatc gtggccaacc cgcagttcca
      361 atttacggtg acaccagcga gcgcaaattt ggccacgaac gcctcattca actacaacat
      421 cacggttaag aacatcggga cattgagctt gagcaacgtc atcatgagca acgcgatgcc
      481 agccggaatt tcttttgact acgtgcagta tggccgcgga tcatgtgata ttgaggacac
      541 ctatctcctt tggtcgctcg gaaacatgaa caccaatgtc agcgcctcca tgaccgtgac
      601 tgccacaact ggtgccaacg gcacttggac taaccttttc ggcgtcgccg attcgcaagg
      661 agcggccacc gctaaagccg ttcaacttct ttacgtggga attacgccgc cagttctgtt
      721 aaacatcgct ctcacgaaca gccaagtcct gttatcttgg ccttcgacca taggaaattt
      781 cggacttcag acgacaacaa atcttgtctc ccaaacaagc tggaccgctg caacaaacgt
      841 gcccgttacc aacggcggta cgattatcgt ggcgcttccg gtgacgagaa cgaatcagtt
      901 cttccgtttg aaggggcaat gaagtttcgc cgggactcgg tgcgattaat tgattcccgg
      961 ttgaatctgg atgaatctgg atgggttggc atgctttttg cgttggaaaa ggcggcacaa
     1021 cctgcaacaa tcctcctgcg ccctgtttcc atcggggcgg gacgtaacga gcaaattgac
     1081 tttcattgat ttattttgcg gttgtggtgg tttcagcctc ggcttgaacc gggccgggtt
     1141 gacgggtttg gccgctattg atttcaatcc cgaagccatt gcggtcttca aaaggaacct
     1201 gcctcaaatc ccgcattcct tgggcaaaga cttgacgggc ttcgcccccg cagcattggc
     1261 gaagctgata ggcacggctg aagttgatgt cattgtgggc ggaccgccgt gccagggatt
     1321 cagcactgtt cggcaggtgg atggcgcgaa tcatggcaaa cgtctggtgg atgacgcgcg
     1381 tcgccatctt taccaggaat tcttacgcta tgtgaatttt ttccaaccaa aagtatttgt
     1441 gatggaaaat gtgctgggca tccgttccgc atccgagggg gagtatttca gtcgggtcca
     1501 aaaagaggcg cgggcgcttg gttatcgggt tcacgcgcaa gtggaagact gcatggaact
     1561 cggcgtgccg caaaagcggc ggcggcaact tttcattggg acacgtcgcg atttgccaga
     1621 ttatttccgc ccggaactca agcctgctcc taggattgac gtttgtcgcg atggaactca
     1681 aggtttgaaa gttcccatca ccttgtggga agcgattggg gacctgcca
//
LOCUS       DASZMM010000315         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5068664, whole genome shotgun sequence.
ACCESSION   DASZMM010000315 DASZMM010000000
VERSION     DASZMM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5068664"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..724)
                     /locus_tag="VG754_07580"
     CDS             complement(347..724)
                     /locus_tag="VG754_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF19111.1"
                     /translation="MKREGSLSKGFTLIEIMIVVAIIGLLAAIAIPNFVHARTNSQQN
                     ACINNLRQIDGAKQTWALDNKVSPTSTPAASLIQLYMGRGTGGALPFCPADTGSTFSS
                     SYSINDLQSPPTCQIVPTLHIYN"
     gene            complement(1037..1249)
                     /locus_tag="VG754_07585"
     CDS             complement(1037..1249)
                     /locus_tag="VG754_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HWF19112.1"
                     /translation="MNRAIGLACLIGGIVLLILGLQASHSFNSDVSRTFTGNPTNNSM
                     WMIVVGIVVAVFGLVMSLRSGSGNRP"
     gene            1402..2784
                     /locus_tag="VG754_07590"
     CDS             1402..2784
                     /locus_tag="VG754_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWF19113.1"
                     /translation="MPLGEKILSDLRRLVGPENVLTQPEDIIPYSFDGTAALQQTPGC
                     VIFALKTEQIVEILKLANQTKTAVVTRGSGTGLSGGSLPSKDCIVLCTVKMDKILELD
                     RANLTMLVEPGVITLSIADAANAANLFYPPDPGSMKISTIGGNVAENSGGLRGLKYGV
                     TRNYVMGMEVVLPEGEVMWLGNKCVKDVAGYSLKDLFIGSEGTLGVISKVLLRLIPKS
                     AAKKTMVATFAQMDQAAEAVSAIIAAQIIPCTLEFLDRMTIHCVEDYAKIGLPLDCEA
                     LLLMETDGHPAVVADEATRMEKCCRDCGCMELRIAQDDAEAAKLASARRSAFSALARV
                     APTTILEDATVPRSELARMIRFVEAVAKKYQLKIGTFGHMGDGNLHPTFLTDERNKAE
                     MHRVEEAFKEIFDEAVRLGGTISGEHGIGLAKKSFLPAFLGDAQMRVLHEFRKVLDPN
                     GILNPGKMFD"
     gene            2791..>3123
                     /locus_tag="VG754_07595"
     CDS             2791..>3123
                     /locus_tag="VG754_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWF19114.1"
                     /translation="MYTAPSPAAHSHLKDLDYSVVQQCMHCGLCLPTCPTYDATKLER
                     NSPRGRIALMRAVADGHLEPTKTFADEMYFCLGCLACMTACPAGVNYAELFEHARAEA
                     EQSGVLNSP"
BASE COUNT          698 a          841 c          863 g          721 t
ORIGIN      
        1 tggccttcat tttcgtgggt gtgctggctt atgatggcca tcgacggcat cggcaaatgc
       61 ttcggcacat gtcgcgttcc agccatgtcg gaacgagcaa tatgatatca aggtcctaca
      121 ggaatttccg ttcgttttac cgttcgctgc atgaggagct tggacgccgc aaaaggcggg
      181 tggaacgggc ccgtggtcgc cgcagatgag cagcggagag acgcgttgat gctgtttgta
      241 accccgctgg cttgtccaac gtcaatttct ggtgataaga agtcccaact aaaaaaagca
      301 ggccggcatc gagtgatgcc ggccttttaa ttttttgcgc ctgcggtcaa ttataaatgt
      361 gcagggttgg gacaatctgg cacgtgggcg gggattgcag gtcattgatg ctgtaactgc
      421 tgctgaaggt tgaccctgta tccgccgggc agaatggcaa cgcgccaccc gtgccgcggc
      481 ccatgtacaa ttggatgaga ctagccgccg gggttgaagt cggggagacc ttattatcca
      541 gcgcccaagt ttgcttggcg ccatcgatct ggcggaggtt gttgatgcag gcattttgct
      601 gcgagttagt gcgggcgtgc acgaagttgg gaatcgcgat ggcagccagc aaaccaataa
      661 tggcgaccac aatcatgatc tcaatcaacg tgaaaccctt tgacaaggag ccttcgcgtt
      721 tcataactat tgacctttca gagcggtctg aacgcggcac caggcggtta tttattatta
      781 ttactaaacg gttgttcaat tattgccgga tgccaaccgc atggcggatt gcaagcaggg
      841 ccaatgccgt acgaagttgt ccagaaatga agcttttgga cgggttttta cctccggaat
      901 tgctccgaat tttcccgatg gccgcacaaa cccggcgaaa agtttgccag aagtgtcttg
      961 aattgacgaa cactgtccaa gaacgggacg ccccgccaaa tccacgattc ttggataaat
     1021 ctgctttgaa attgcgtcac ggacggtttc cagacccgct gcgcaagctc atgacgagtc
     1081 caaacacggc cacaacgatt ccaacaacga tcatccacat cgaattgttg gtcgggtttc
     1141 ccgtgaaggt gcgcgaaaca tccgagttga atgaatgcga ggcttgcagc cccaaaatca
     1201 acagcacaat gcctccaatc aaacatgcca atccaatagc gcgattcatg tggttccttt
     1261 cagttgggtt atactattcg ccgcctgatg cgtccgccat ggggtgtttt ccgttcgagc
     1321 atgggtgttt ggaagatacc gtttgtgcgc aaggtgcttg accctgccgg aacgctcgaa
     1381 catcctcatg ctgatttgat tatgccgctg ggcgaaaaga ttctttccga tctgcgccgg
     1441 ctggtcgggc ctgaaaatgt gctcacccag ccggaagaca tcatccccta ctctttcgac
     1501 ggcacggctg cgctgcaaca gacgccgggt tgcgtcattt tcgcgctcaa aactgagcag
     1561 atcgtggaaa ttctcaagct tgccaaccaa accaagactg cggttgtcac gcgcggttct
     1621 gggactggtt tgagcggcgg cagcctgccg agcaaggatt gcattgtgct ttgcacggtc
     1681 aaaatggaca aaattctcga actcgatcgc gcaaacctca cgatgctggt tgagccggga
     1741 gtgatcacct taagcatcgc cgatgccgcc aacgctgcga acctgtttta tcctccggat
     1801 cccggctcga tgaaaatttc caccatcggc ggcaatgtgg ccgagaattc cggagggttg
     1861 cgcggcttga aatacggcgt gacgcgcaat tacgtcatgg gcatggaagt tgtgctgccg
     1921 gagggcgaag tgatgtggct tggcaataaa tgcgtgaagg acgttgcggg ctattcgctg
     1981 aaagatttgt tcatcggctc ggaagggaca ttgggcgtca tcagtaaagt gttgttgcgc
     2041 ttgattccca aatcggcggc caaaaaaacc atggtggcca ccttcgcgca aatggaccag
     2101 gcggctgagg ccgtttcggc catcatcgcc gcgcaaataa ttccctgcac gctggaattt
     2161 ttggaccgca tgacgattca ttgcgtggag gattacgcca aaatcggttt gccgctcgat
     2221 tgcgaagcct tgttgctcat ggaaacggac ggtcatccgg cagtggtcgc cgatgaagct
     2281 acacgcatgg aaaaatgctg ccgtgattgt ggctgcatgg aacttcgcat cgcccaagat
     2341 gatgctgaag ccgccaagct cgccagcgcc cgccgttcgg ctttttccgc gcttgcgcgc
     2401 gttgcgccga cgaccatcct cgaagacgcg accgtccccc gcagcgaact ggcgcggatg
     2461 atccgcttcg tggaagcggt tgccaagaag tatcaactca aaattggcac gttcggccac
     2521 atgggcgatg gcaatttgca tcccactttt ttgactgacg agcgaaacaa ggccgagatg
     2581 caccgcgtcg aggaggcatt caaagagatt ttcgacgaag ccgtccgtct cggtggcacc
     2641 ataagcggcg aacatggcat cggcttggca aaaaaatcat ttctgcctgc gtttctgggt
     2701 gacgcccaga tgcgcgtcct ccacgaattt cgcaaggtgc tcgacccgaa cggcatcctg
     2761 aatccgggga agatgtttga ttaggttatt atgtacactg ctccttcccc tgccgcacat
     2821 tctcacctga aagaccttga ttattcggtc gtgcaacaat gcatgcactg tggtttgtgc
     2881 ctgcccacct gccccactta cgacgccacg aaactggaac gcaacagccc gcgcggacgc
     2941 atcgcgctca tgcgcgccgt cgccgatgga catcttgaac caaccaagac ctttgccgat
     3001 gaaatgtatt tctgccttgg ttgtctcgct tgcatgaccg cctgtcccgc aggcgtcaat
     3061 tatgccgagc tttttgaaca cgcccgcgct gaagctgaac aatccggcgt gctcaattca
     3121 ccc
//
LOCUS       DASZMM010000316         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2045594, whole genome shotgun sequence.
ACCESSION   DASZMM010000316 DASZMM010000000
VERSION     DASZMM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045594"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VG754_07600"
     CDS             <1..756
                     /locus_tag="VG754_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWF19115.1"
                     /translation="PTGSGKSTSLYAFLSSINSVHKRIITIEEPVEYELKGINQIAVR
                     SDIGLTFAMGLRHILRQDPNVIMVGEIRDLETAEIAIRAALTGHLVFSTLHTNDAPSA
                     FTRLIDMGIEPFLVASSVEAIMAQRLVRNICQHCKTEQKVERDYLRKIGFPAADLETA
                     KFWRGAGCEECRQLGYQGRQGIYELLILNEAIRPLILNRSPASTIAAKAIDGGMRTLR
                     TDGWNKVKAGRTTIEEVLRVTQIEEHLDSLSDD"
     gene            812..2560
                     /locus_tag="VG754_07605"
     CDS             812..2560
                     /locus_tag="VG754_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19116.1"
                     /translation="MSTTSNTQSKGVKWFCLLIGIMLVLAALGWLGWNGYLAWLVWKA
                     QHVKGKPVPPQALSNLIAAYAGKLMGGIIGLFAGIWLLQFGNKKTGSSLVAEATAVQN
                     LQSHTALPELAVKPQHRAKARRWTSCNVLAVGADVRRLWTFNSGKNGFMVAQQAAIPS
                     AQPLPARTVGRDWRNLPLLQPKLNIAWLPIEQVFLRVAHLPVSNFDETLSMVELQLEK
                     LSPLPVTQIVWSVQILPQIVDNLQTVIVIIVARDLVEKFLGQLEGQGFLADRLELPIV
                     DELLATRITHDGAYIYPDNSTGKYSALVAWWYGGTLRNLGLLHVPAADNRADILKDQL
                     AQMTWAGELEGWLSGTPRWSLIANDATAANWQPMFRPWLGQNVEVLPPLSEADLATRN
                     ANRAARAESSTGILPVEYALRYAQEFHDRLWMRGLGTIVVVYLIGVMVYMAGASWKGM
                     QADNLEMRMKGLAREYTNTLQVKAQVEILQNRQALKFASLDCWKTTAELLPAGITLGS
                     MDFKEGKTLSLSGTAPGDANGTLTDFNEAMRKFTRPDGQLMFERVDLPSVRLNAGGGT
                     LSWGFSATLANAEETQ"
     gene            2557..3333
                     /locus_tag="VG754_07610"
     CDS             2557..3333
                     /locus_tag="VG754_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19117.1"
                     /translation="MKRYWEGLKPLERRWAVGAGCLVFLMLNYFFIWPHRHDWARDDT
                     RTHAAEAKIATYNAEIAKKSAYLTKLRNLQAMGDQVLPEDQAIDFVHFYSSRMLSNNI
                     LFLSGGTLTTRTNSPFFMEQQLGISVQANETNLVNFLYSLAAGNSMVRVRSMSLHPNP
                     DRHELSAGITMVASYQKKVSATASKNATPPLTPVKNVIQSPRPAAPAIQPVNNHPAPP
                     PMMGSKLPPPPAMNMAAMSNRMAHFLKPGMTNRPPQKNAN"
     gene            3336..>4450
                     /locus_tag="VG754_07615"
     CDS             3336..>4450
                     /locus_tag="VG754_07615"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWF19118.1"
                     /translation="MKTTLLILFAACFSVWAQTSSTDLNGIDDAARDAALRRAMRAAA
                     NSNAVGAGPTLAANTVPSNGPAPVGTSNYFPAAPNTPTALPTTGINPSAFAGSTNTNN
                     VRRVFPRNNRAGQAPGFNPAAANPVPGFNPAGNQPPAFNPATGSPVPSLPAAPAATPA
                     VPEEVIGAGKIDFPQAPLDAVLDIYAELVGRTILHGQLPPVQVTLKTETALTRTEAIQ
                     ALDSVLAMNGITMVPIGDKFVKAVQGNQVFQTAPKFLTHDAEQLPEADQYITYITQLK
                     FAKPSELVAVLAPFAQIPNSILAIDSSQMLVIRDYAGNVKRMLELIKQIDVTVPLDYD
                     SEVIPIKYAKASDIASALSSLGGGTGTSIGHSAGTGT"
BASE COUNT          989 a         1322 c         1244 g          895 t
ORIGIN      
        1 ccgactggtt ccggcaaatc gacttccctc tatgcctttt tgagcagcat caattccgtc
       61 cacaagcgca tcatcacgat cgaggagccg gtggaatatg aattgaaagg catcaaccag
      121 atcgcggtgc gttcagacat cgggctgacg ttcgcgatgg gtttgcgcca tattttgcga
      181 caggacccga acgtcatcat ggtcggggaa atccgcgact tggaaacggc ggaaatcgcc
      241 atccgcgccg cgttgaccgg tcacttggtg ttcagcaccc tgcacaccaa cgacgcgccc
      301 agcgcattca cgcgtttgat tgacatgggc atcgagccgt tcctcgtggc gtcgtccgtg
      361 gaagcgatca tggcgcaacg cctcgtgcgc aatatctgcc agcattgcaa gaccgagcaa
      421 aaggttgaac gcgattacct gcgcaaaatc ggtttccccg cggcggacct cgaaacggcg
      481 aaattctggc gcggcgcggg ttgcgaagaa tgccggcagt tgggttatca ggggcggcaa
      541 ggcatttacg aattgttgat tttgaatgaa gccatccgac cgctcatcct caaccgctcg
      601 cctgcttcga ccatcgctgc caaggcgatc gatggcggga tgcgcacgct gcgcacagac
      661 ggttggaaca aggtcaaagc cggccgcacc accatcgagg aagtcctgcg cgtgacgcaa
      721 atcgaagaac atctggactc gctttccgac gactgaaatc catccgcatg aagcaaatcc
      781 gtaattccac gacgaaaggc tttttctccc gatgagcacc acttcgaata cccagtcgaa
      841 aggggtcaag tggttttgcc tgttgattgg catcatgctc gtgctcgccg cgttgggctg
      901 gctgggctgg aatggctatc tggcgtggct cgtgtggaaa gctcagcatg taaagggcaa
      961 gccagttccg ccccaggcat tgtccaatct gatcgccgcg tacgcaggca aactcatggg
     1021 cggaatcatc ggactttttg cgggcatttg gctgctgcaa tttggaaata aaaagaccgg
     1081 ttcttcgctg gttgcggaag cgactgcggt ccagaatttg caatcccaca ctgcccttcc
     1141 cgaactcgcc gtcaaaccac aacatcgcgc caaggccaga cgctggacga gctgcaatgt
     1201 gctcgcagtc ggcgcggacg tccggcgact ctggactttc aactcgggaa agaatgggtt
     1261 catggtcgca cagcaggcgg cgatcccgtc ggcgcagccg ttgccagcca ggacggtggg
     1321 acgcgactgg cgaaatttgc cgctactcca gccgaagctg aatatcgcat ggctgccgat
     1381 cgaacaggtc tttttgcgtg tcgcgcacct tccggtgagc aattttgacg aaacgctctc
     1441 gatggttgag ttgcagttgg aaaagctttc gcccctgccc gtgacgcaga ttgtctggag
     1501 tgtgcagatt ttgccgcaga tcgtggacaa tttgcagacc gtgatcgtca tcatcgttgc
     1561 gcgcgatctc gttgaaaagt ttctgggtca attggaagga caaggttttc tggctgaccg
     1621 tctcgaactg ccgatcgtgg acgaattgct tgccacccgc atcacgcacg acggcgctta
     1681 catttatcct gacaattcca ccggcaaata cagcgcgttg gtggcctggt ggtacggcgg
     1741 cacgttgcgg aacttgggct tgctgcacgt tcccgccgcg gacaaccgcg ccgacatcct
     1801 gaaggaccaa cttgcgcaaa tgacctgggc cggggaattg gaaggctggc tcagcggcac
     1861 accgcgttgg agcctcatcg ccaacgacgc caccgccgca aactggcagc cgatgttccg
     1921 tccctggttg ggtcaaaatg tcgaagtgct gccgccattg tccgaggcgg acctcgccac
     1981 gcgcaacgcg aaccgcgcgg cccgcgcgga atcgtccacg ggcatcttgc cggttgaata
     2041 tgctttgcgt tacgcgcagg aatttcatga ccgcctctgg atgcgcggtc tgggaaccat
     2101 tgtggtggtg tatctgatcg gtgtgatggt gtacatggcg ggcgcttcat ggaaagggat
     2161 gcaggcggac aatttggaga tgcggatgaa gggccttgct cgcgaataca ccaacacgct
     2221 ccaagtcaag gcgcaggttg aaatcctgca aaaccggcag gcgttgaaat tcgcttcgct
     2281 ggattgctgg aagaccactg ctgagttgct gcccgccggc atcacactgg gctccatgga
     2341 ttttaaggaa ggcaaaaccc tcagcctgag cggcaccgcg cccggcgacg ccaatggaac
     2401 gttgacggat ttcaatgaag ccatgcgcaa atttaccagg ccggacggcc aattgatgtt
     2461 cgaaagagtt gatttgccga gcgtgaggct gaatgcaggc ggcgggactt tgtcatgggg
     2521 cttctcagca accttggcca acgcggagga gacgcaatga agcgttactg ggaaggatta
     2581 aagcctttgg aacgccgctg ggccgtgggc gcgggctgcc tagtgttcct catgctcaac
     2641 tactttttca tctggccgca tcgccatgat tgggcgcgcg acgatacaag gacgcatgcc
     2701 gccgaggcaa agatcgcgac ctacaatgcg gaaatcgcga aaaagtcagc atatctcacc
     2761 aagctgcgca acttgcaagc catgggtgat caagttttgc ctgaggacca ggccattgac
     2821 ttcgttcatt tttacagcag tcgcatgttg agcaacaaca tcctgtttct gagcggtggg
     2881 acgcttacta cacgcaccaa cagtccgttc ttcatggagc agcaactcgg catcagcgtg
     2941 caggcgaatg aaacgaacct ggtaaatttt ctctacagcc tcgccgcggg caattcaatg
     3001 gtgcgcgtcc gctcaatgag ccttcatccc aatcctgatc gccacgaact cagcgcggga
     3061 atcacgatgg ttgccagcta ccaaaagaag gtgtcggcaa ccgcttccaa gaatgccacg
     3121 cctccattga cgccggtcaa gaacgtcatc cagtcgccca gacccgctgc accggcaatc
     3181 cagcctgtca acaaccatcc ggcaccgcct cccatgatgg gaagcaaatt gccgccgcct
     3241 ccagccatga acatggccgc gatgtcaaac aggatggccc attttttaaa accgggaatg
     3301 acaaacagac ctccacaaaa aaacgctaac tgaccgtgaa aacaacactg ctcattttat
     3361 tcgcagcctg cttcagcgtg tgggcacaaa ccagttccac cgacctcaac ggcatcgatg
     3421 acgccgcccg cgatgcggca ttgcgccgcg cgatgcgcgc cgctgccaat tcgaacgctg
     3481 tcggcgctgg cccgaccttg gctgccaaca cggtgccctc caacggcccc gcgcctgttg
     3541 gcacgagcaa ttattttccg gctgctccga acactcccac ggctctgccc accaccggca
     3601 tcaatccgtc cgcttttgcc ggaagcacaa atacaaacaa cgtccggcga gttttccctc
     3661 gcaacaatcg cgcgggccaa gcgcctggtt ttaaccctgc tgcggcaaat cctgtgcccg
     3721 gcttcaatcc ggctggcaat cagcctccgg cgttcaatcc cgcaacagga agtccagttc
     3781 ccagtcttcc cgccgcacca gcggcaactc ccgccgttcc ggaagaggtc attggcgcgg
     3841 gtaagattga ttttccgcaa gcgccgcttg atgctgtgct cgacatttac gccgaactgg
     3901 tgggacgcac cattctgcac ggccagttgc cgccggtcca ggtcactttg aaaaccgaga
     3961 cggcgctcac aaggacggaa gcgatccaag ctctggacag cgtgcttgcc atgaacggca
     4021 tcaccatggt ccccatcggt gataaattcg tgaaggcagt gcagggcaac caggttttcc
     4081 agaccgcgcc aaagtttttg acgcatgatg ccgagcaatt gcctgaagcc gaccaataca
     4141 tcacttacat cacgcaactg aaatttgcca agccgagcga attggtcgcc gtccttgcgc
     4201 cgttcgccca gataccgaac agcattctcg cgattgacag cagccagatg ctggtcatcc
     4261 gcgattacgc gggcaatgtg aaacggatgc ttgaattgat caaacagatc gatgtcaccg
     4321 tgccgcttga ttacgactcg gaagtcatcc ccatcaagta tgcgaaagcc tcggacatcg
     4381 cgagcgcgtt gagcagcctc ggcggcggca ctggcacgag catcggccac agcgccggca
     4441 ccggaaccca
//
LOCUS       DASZMM010000317         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2046764, whole genome shotgun sequence.
ACCESSION   DASZMM010000317 DASZMM010000000
VERSION     DASZMM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046764"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VG754_07620"
     CDS             complement(<1..641)
                     /locus_tag="VG754_07620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWF19119.1"
                     /translation="MHRFYLPPAQCKDKTLILTESEAHHALHVLRIRDGERLMVLDGA
                     GQELLCEAHRPEGGQLKLTVVQRNIVPPLPYQITLLQAIPKGKIIEGIIQKATELGAH
                     RIVPLLSDRVTTQLDDESAATKAEKWKQVAIEAIKQCGNAWLPQVEAPITPKAYLARN
                     EKFELPLIASLQSDCRHPREYFKTFYSEKKRLPGTVCVWVGPEGDFTPAEMSAV"
     gene            complement(695..1819)
                     /gene="dnaJ"
                     /locus_tag="VG754_07625"
     CDS             complement(695..1819)
                     /gene="dnaJ"
                     /locus_tag="VG754_07625"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWF19120.1"
                     /translation="MAKRDYYEILGVERGVEIEEIKKAYRKLAVKYHPDKNPGDKAAE
                     EKFKELGEAYEALSDPQKRAAYDQYGHAAFDPRMRSGSRAGGFHDPADIFREVFSGSM
                     FEDLFGGRSDPTQPQRGADLRYDMEITFEEAAHGCEKEITVSKQDRCETCHGSGAEPG
                     SRTKTCPTCNGRGQVISSRGIFSIAQTCPRCEGAGKVIEKPCKICHGAGRHERTSKIK
                     LRIPAGVDTGSRLRSSGNGEAGMRGGPVGDLFVVLHVKPHEIFQRDGDDLICDVPVSF
                     VQAALGSEIDVPTLSGETPIKMPAGTQSGSVFRLKGKGIKNIQGYGIGDLHVRVNVEV
                     PSHLNAAQKAKLQEFAELCDETVNPISKSFFEKAKHLFGG"
     gene            complement(1823..2407)
                     /gene="grpE"
                     /locus_tag="VG754_07630"
     CDS             complement(1823..2407)
                     /gene="grpE"
                     /locus_tag="VG754_07630"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWF19121.1"
                     /translation="MKKRNHTMSKARESTDIATETAENPSTTALVPEPLTPEQIEDLK
                     TRAERADENWQRLLRATADLENYKKRAQREREDAVKFANESLMKKLIPVLDNFEMALA
                     AAQKTDTANAQSLQTGVAMILQQLRSALMESGLEEVDAAGKTFDPNLHEAVSQQESAD
                     VPEGQVLQQLRKGYKLRDRLIRPATVVVAKKPAA"
     gene            2676..>3215
                     /locus_tag="VG754_07635"
     CDS             2676..>3215
                     /locus_tag="VG754_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19122.1"
                     /translation="MDMEQGGTPPIISEVGAETKTVSRGRWWIHLGVLTIFPLVPAVM
                     GLVYRQSHHTILPKTVGGLLGIALFELMFFVLLFGFAWLASRANADQLLLHWRRGVLP
                     VVLGFAYSFALRLAIMVLLVAVVFAWWLLTAIHNGFQNGINPTDIQQFRPQTEQLINA
                     QALVNSPIYLVICLTLISFV"
BASE COUNT          613 a          936 c          882 g          784 t
ORIGIN      
        1 accgcgctca tttctgcggg cgtgaaatcg ccttcgggac cgacccagac gcaaacagtt
       61 ccgggcagcc gctttttctc agaataaaac gttttgaaat attcgcgtgg atgtcggcaa
      121 tcgctttgca atgaggcgat caagggcagt tcaaattttt cgttgcgcgc cagataagcc
      181 ttgggcgtga tcggcgcttc aacttgcggc agccacgcgt tgccgcattg cttgatggct
      241 tcgatggcaa cctgcttcca tttctcggcc ttggttgcgg cgctttcatc gtccaactgc
      301 gtcgtcacgc ggtccgagag caggggcacg atgcgatgcg cgcccaactc ggtcgctttt
      361 tgaatgatgc cctcgatgat tttgcccttg ggaatcgctt gcaaaagggt gatctgatac
      421 ggcaacggcg gaacaatgtt gcgttgcaca acggtgagct tcaactgtcc gccttcgggc
      481 ctgtgcgctt cgcaaagcag ttcctgtccc gcgccatcca agaccatcaa gcgctcacca
      541 tcccgaatgc gcaacacgtg caacgcatga tgcgcctcgc tctcggtgag aatcagcgtc
      601 ttgtccttgc attgggcggg tgggagatag aaacggtgca tgattagaag tttaaagttc
      661 cgagttcaaa gtttaaagtt gaggacgagc gaactcaccc gccaaaaaga tgtttggcct
      721 tctcgaaaaa actcttgctg atggggttca cggtttcgtc gcacaactcc gcgaactcct
      781 gcagcttggc tttttgcgcg gcgttgagat gcgaaggcac ttccacattc acgcgaacat
      841 gcaggtcgcc tatgccgtag ccctggatgt ttttgatgcc tttgcccttg agccggaaca
      901 cgctgccgga ctgggtgcct gccggcattt tgatgggcgt ttcgcccgag agcgtcggca
      961 cgtcgatttc agagccaagc gccgcctgga caaaactgac gggcacatcg caaatcaaat
     1021 catcgccgtc acgttggaaa atttcatgcg gcttgacgtg caacaccacg aaaagatcgc
     1081 ctacgggacc gccgcgcatg cccgcttcgc cgttgccgga agaacgcagt cgcgacccgg
     1141 tgtccacgcc cgcgggaatg cgcaacttga tcttggaagt gcgctcatga cgtcccgcgc
     1201 catggcaaat tttgcacggc ttctcaatga ccttgccggc gccttcgcaa cgggggcaag
     1261 tctgcgcgat gctgaaaatt ccgcgcgaac tgatgacttg tccgcgcccg ttgcaagtgg
     1321 ggcaggtctt tgtccgcgag cccggttccg cgccggagcc gtggcaagtc tcgcagcggt
     1381 cctgtttgct gacagtgatt tccttctcgc aaccgtgcgc ggcctcttcg aaagtgattt
     1441 ccatgtcgta acgcaagtcc gcgccgcgtt gcggttgcgt cgggtcggag cggccgccga
     1501 aaaggtcctc gaacatgctg ccgctgaaaa cctcgcggaa aatatccgcc ggatcatgga
     1561 acccgccggc gcgcgacccg ctgcgcatcc ttggatcaaa agcggcgtgg ccatattgat
     1621 cgtaggcggc gcgtttttgg ggatcgctca aggcttcgta agcttcgccg agttccttga
     1681 atttttcctc cgccgccttg tcgccggggt tcttgtccgg atgatatttg acagccagtt
     1741 tgcggtaggc cttttttatt tcctcgatct caacgccgcg ttcgacgccg agtatttcgt
     1801 aataatctcg tttcgccatg ggttaagccg ccggtttttt ggccaccacc acagtggccg
     1861 gcctgataag ccgatcacgc aacttgtaac ccttcctcaa ctgttggagc acctggcctt
     1921 ccggaacatc cgccgattcc tgctgcgaaa ccgcttcgtg caggttcggg tcgaaggtct
     1981 tgcccgctgc gtcaacctct tccaaccccg actccataag cgcgctgcga agctgctgca
     2041 aaatcatcgc gacgccggtc tggagggact gggcgttcgc cgtgtccgtt ttctgggcgg
     2101 cggcaagcgc catctcgaaa ttgtccagca ccggtatcaa ttttttcatc aacgactcgt
     2161 tggcgaactt caccgcgtcc tcgcgttcgc gttgggcgcg tttcttgtaa ttctccaaat
     2221 cggccgtggc ccgcaacagg cgctgccaat tttcatcggc gcgttccgcg cgcgttttca
     2281 aatcctcgat ctgctctggc gtgagcggtt ctggcaccaa cgccgtggtg gacgggttct
     2341 cggccgtttc cgtggctata tctgtgcttt ctctggcttt tgacattgtg tgattccgtt
     2401 ttttcacgat tttggtgaaa cacctgacac ccccaaatta tacgattgtg tggagatggg
     2461 caaccccgat gtgtatcaac cccagttggc caggattcaa caaatgataa cgctgaacat
     2521 caaacaccga acgcccaacg cagaaagaga ggcactcggg ccatggatgt tcagcgttcg
     2581 atgtttagcg ttcagcgttt tcatcccatc agggaaatcg gacttggctt aagtttggct
     2641 cgacgttcct gcatcgccaa ggacatgctg gagtcatgga tatggaacag ggcgggacac
     2701 ctcccatcat cagcgaagtt ggcgcagaaa ccaaaactgt ttcacgcggt cgctggtgga
     2761 tccatctggg cgtgctcacg attttcccgc tcgtccctgc cgtgatggga ctggtctatc
     2821 gccagtcaca tcataccatc ctgcccaaga cggtcggagg cttgctggga atcgccctgt
     2881 tcgaactgat gttcttcgtc cttctttttg gatttgcatg gctggcgtcg cgggccaatg
     2941 ccgatcaact ccttttgcat tggcgtcgcg gcgtgctgcc ggtggtcctc ggattcgcgt
     3001 attcattcgc gttgcgactc gcaatcatgg tgcttctggt cgcagtggtg tttgcatggt
     3061 ggctgctcac ggccatccac aacggttttc aaaacggaat caacccgacc gacattcaac
     3121 aattccgtcc gcaaacggaa caattgatca acgcccaggc attggtcaat agcccgattt
     3181 atctggtcat ctgcctgacg cttatcagct tcgtc
//
LOCUS       DASZMM010000318         4681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2046770, whole genome shotgun sequence.
ACCESSION   DASZMM010000318 DASZMM010000000
VERSION     DASZMM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046770"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..2501)
                     /locus_tag="VG754_07640"
     CDS             complement(258..2501)
                     /locus_tag="VG754_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWF19123.1"
                     /translation="MKWSRGGFLAIFFICWTCQFAKADWAPGHAPLMTRWAAEVGPTN
                     SHPEYPRPQLVRPDWLNLNGLWDYAIRPTTSVRPANFDGQILVPFPVESALSGVMKTL
                     DENSALWYRRTVKIPPEWRGRRVRLNFGAADWQCRVFVNGREAGGHRGGYGRFAFDIT
                     GSLRWTNGEEIVVAVTDPTEADQPRGKQSRKPEGIFYTPTSGIWQTVWLEPVPDGCID
                     ELRMTPDLDAKGLRLRVTVANVAEDIRVEATALDGTNQVGNVQGQANVELFLPIAQPK
                     SWSPSDPFLYDLHVTLKRGDREVDRVSSYFGMRSVSLQKDDKGIPRIALNGQPFFELG
                     TLDQGMWPDGIYTAPTDEALKYDLEFLKSAGFNLARKHVKIEPDRWYYWCDKLGLLVW
                     QDMPSGNNATVEGQREFESELLHMVKDLYNHPSIIVWVLFNEGWGEYNTQRLTQRLKT
                     LDSSRLVDNASGWADARVGDLADTHSYPGPSPPESESTRAVVLGEFGGLGLVMDGHSW
                     PSSRDWAYRMEPDVPTLAKSYASLVREVRSMNAIQGLSAAVYTQTTDVETECNGLLTY
                     DRTSKLNPNFLAEANLSARFAEAPKVIVEDATSGLPEWKYTFKAPETNWVEPQFNDAD
                     WQRGPAGFGTPRTPGAIPTTLWSTDDIWLRRKFVLGNEAMSGAKLEAHHDEDAEIYLN
                     GVLAAKLPGYITHYDLFDILPDAAATLKPGANTMAVHCHQTTGGQYIDAGLVLPTKGT
                     NEIKAGP"
     gene            complement(2498..3136)
                     /locus_tag="VG754_07645"
     CDS             complement(2498..3136)
                     /locus_tag="VG754_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF19124.1"
                     /translation="MTKPIKISLVDDSPDLRENITGYLEPFPEFKCVSTYASGEEALA
                     GLQKDAPDIVLMDINLGEHAMDGIECVRKLKKLMPEVQVVMLTVFEDSEKIFRTLAAG
                     ASGYLLKRQKPGELVSAIKEVVAGGAPMSASIARIVVKSFQSQPSHGPEADALSHREQ
                     EVLEGLAQGLAYKQIADQLNVSIHTVRNYIRRIYEKLHVRSSAEAVAKYLKP"
     gene            complement(3133..>4681)
                     /locus_tag="VG754_07650"
     CDS             complement(3133..>4681)
                     /locus_tag="VG754_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HWF19125.1"
                     /translation="LSPRLAGKAILGLCESTNGGLWIGTDGGGLGYVNKDVRRAWDVQ
                     DGLPDNVVWGLTEDDEANLWLATPRGLCRISSDSINRALARPEPLKVKLVYETDQSTP
                     KALRFGGPHAIRSAGGRLWFALNSGPVGVDIRGRESDKPAPQVHIENLLVDNQPVKFS
                     AEKPTLATSDAHADVLMLPGNPKTLEFHFVGLSFDAPEKVRFRHKMDGIDADWVQNGT
                     ERQIGYGPLPSGNYVFHVTACNAEGIWNDKGASLAFIIPTPLWRQPWALGMGGLTATF
                     LVVGMVRVVSHRRLRWRLEHLEQQRAMERERIRIAQNMHDEIGSKLTKISYLSERAKT
                     ELRGAGKAEGKIDSIATTSRELLKALDEIVWAVNPKNDSLEHLAAYFCQYAREYFQDT
                     TVECDIRMQSKLPEMEMSAEMRHNLFLAFEESLNNILKHAKASSLNMDISTDADRLRI
                     TIRDNGRGFEQKADSNGKATGGNGLRNIRHRMASVGGTCEVESAPGRGTCISLRVPLD
                     SAKIRNR"
BASE COUNT          956 a         1351 c         1286 g         1088 t
ORIGIN      
        1 agggcaggtt tcattcatgc ccctccttct aactgataac cccactcttt gcccgctcaa
       61 aaactgagat gcgccccatt tcacgttggt gaatatgttg ttttatggtg agtgttttgc
      121 tttggttgga gtaatgttca ggatggttga agtaatccta cagccatcaa agcaagcttt
      181 ggaaaatgtc tgccaacatt tcagggcgga agataatttt ttcgctctga aaatagtttg
      241 tgatcacctg ccggttttta gggtccagct ttgatttcgt tcgtgccctt ggtcggcaac
      301 accaaacccg cgtcaatata ttgtccgccc gttgtctggt ggcagtgaac agccatcgtg
      361 ttggcgccgg gtttgagggt tgcggcggcg tccggcagga tatcaaaaag atcgtagtgg
      421 gtgatgtaac cgggaagttt cgcggcaagc acgccgttca gataaatttc cgcgtcctcg
      481 tcgtgatgcg cttcaagttt cgcgccggac atggcctcat tgccaaggac aaactttctt
      541 ctcaaccaga tgtcgtccgt gctccataag gtggttggaa ttgcgccggg cgtgcgcggc
      601 gtgccaaatc ccgccggacc gcgctgccag tcagcatcgt tgaattgcgg ttcgacccaa
      661 ttggtttccg gcgctttgaa cgtgtatttc cattctggca gtccggatgt ggcgtcctcg
      721 acgatcacct ttggcgcttc ggcaaaacgc gcggaaaggt tggcctcggc gagaaagttc
      781 ggattcagct tggaggttcg gtcgtaagtc aaaagcccat tgcattccgt ctcgacgtcc
      841 gtggtctgcg tatagacggc ggcgctcaaa ccctggatgg cgttcatgct cctgacttcg
      901 cggacgaggc tggcgtaaga ttttgcgagc gtcggcacgt ccggttccat gcggtaggcc
      961 caatcgcggc tgctcggcca tgaatgcccg tccatgacca aaccgaggcc gccaaattca
     1021 ccaaggacca ccgcgcgcgt cgattcggat tccggcgggc ttgggccggg ataggaatga
     1081 gtgtcggcca aatcgcccac acgcgcatcg gcccagccgc tggcattgtc aacgagtctc
     1141 gaagaatcca aagtcttgag acgctgcgtc aatcgttgcg tgttgtattc tccccatccc
     1201 tcgttgaaca aaacccaaac gatgatggac ggatggttgt agaggtcctt gaccatgtgg
     1261 agcagttcgg attcgaattc gcgctggcct tcaacggtgg cattgttgcc gctgggcatg
     1321 tcctgccata caagcaagcc cagcttgtcg caccagtaat accagcggtc aggctcgatc
     1381 ttgacatgct tgcgggcgag attgaatccg gcggacttca agaattccaa atcgtatttg
     1441 agcgcttcgt cggtgggagc ggtgtagatg ccgtcgggcc acatgccctg gtcaagcgtg
     1501 cccagttcga aaaagggctg gccattcaaa gcgatgcgcg gaataccctt gtcgtccttc
     1561 tgcaaggaga cgcttcgcat cccaaaataa ctcgatacgc gatccacttc ccggtccccg
     1621 cgtttgagcg tgacgtgcaa gtcatacaaa aatggatcgg atggcgacca cgatttcggt
     1681 tgtgcgattg gcagaaataa ttcgacgttg gcctggccct gcacgtttcc gacctgattg
     1741 gttccgtcca gtgccgtggc ttcaacacgg atgtcctcgg cgacattggc cactgtcacg
     1801 cgcagacgca ggcctttggc gtctaaatcc ggggtcatcc gcaactcgtc gatgcaacca
     1861 tcaggcacag gctccagcca gacggtttgc cagatgcccg aagttggggt gtaaaatatg
     1921 ccttcgggtt ttcgcgattg tttcccgcgc ggctggtctg cttcagtcgg gtcagtcacg
     1981 gcgaccacaa tttcctcacc gttcgtccag cgaagcgagc cggtgatgtc gaacgcaaac
     2041 cgcccatatc ctccacgatg cccgccagct tcgcggccat tcacaaaaac ccggcattgc
     2101 caatctgccg cgccgaaatt gagccgcacc ctgcgaccgc gccattcagg cgggatcttg
     2161 acggtgcgcc gataccaaag cgcactgttt tcatccaatg ttttcatcac gccggacaag
     2221 gcggattcga caggaaatgg aacgagaatt tgtccgtcga aattggcggg ccgaactgaa
     2281 gtcgtgggcc gaatcgcgta atcccaaaga ccattgagat tcaaccaatc cggccggacc
     2341 aattgcgggc ggggatattc aggatgggaa ttggtcgggc cgacttcagc cgcccaacgc
     2401 gtcatcaacg gcgcatgtcc cggcgcccag tcggcttttg cgaattggca agtccagcaa
     2461 atgaagaaga ttgccagaaa tccaccgcga ctccatttca tggcttgaga tattttgcga
     2521 ccgcctcggc gctggagcga acgtggagtt tttcgtaaat ccggcggatg tagtttcgga
     2581 cggtgtggat gcttacattg agctgatcgg cgatctgttt gtaggcgagc ccctgcgcca
     2641 atccttccaa cacctcctgc tcgcggtgtg ataatgcatc cgcctccggc ccgtgggatg
     2701 gctgcgattg aaaagatttc acgacaatgc gggcgatgga cgcggacatg ggcgcgccgc
     2761 cggcaaccac ttccttgatg gccgaaacca attcacccgg tttctggcgt ttcaacaaat
     2821 agccgctcgc accggcggcc agcgtccgaa aaattttttc cgaatcttcg aacacggtga
     2881 gcatcaccac ctggacttcg ggcatcaatt ttttcagctt gcggacgcat tcgatgccat
     2941 ccatcgcgtg ttcgccaaga ttgatgtcca tgagcacgat gtcgggcgcg tccttttgca
     3001 ggccggccaa cgcctcttcg ccgctggcgt aggtgctcac gcatttgaat tcggggaaag
     3061 gttccagata accggtgatg ttctcgcgca ggtccggcga gtcatccaca agggaaatct
     3121 tgatgggctt tgtcatctat tacggatttt ggctgaatcc agcggcactc tcaagcttat
     3181 acaagtgccc cggccggggg cgctttcgac ctcgcaagtt ccgccaacgg acgccatgcg
     3241 gtgccggatg ttccgcaaac cattgccgcc tgtcgccttg ccgtttgaat ccgctttttg
     3301 ttcgaagccg cggccgttgt cgcggatcgt gatgcgcaga cggtccgcat cagtcgaaat
     3361 atccatgttc aaactcgacg ccttggcgtg cttgagaatg ttgttcaacg actcctcgaa
     3421 agctaaaaac aggttgtggc gcatttccgc cgacatctcc atctcaggca gcttgctttg
     3481 catgcgaatg tcgcattcca ccgtggtgtc ctgaaaatat tccctcgcgt actggcagaa
     3541 gtaggcggcg agatgttcca agctgtcgtt cttcggattc acggcccaaa cgatttcatc
     3601 caatgccttg agcaattccc gcgatgtggt ggcgatcgaa tctatttttc cttccgcctt
     3661 gcccgccccg cgaagctcgg tcttggcccg ttcgctcaag taagaaatct tggtgagctt
     3721 ggaaccaatt tcgtcgtgca tgttttgggc gatgcggatg cgttcgcgct ccatggcgcg
     3781 ttgttgttcc aaatgctcca agcgccatcg cagccgccgg tgcgaaacca cgcgcaccat
     3841 gcccaccacc aggaatgtcg cggtcaatcc gcccatgccc agtgcccacg gttgccgcca
     3901 caaaggcgtg ggaatgatga aagcaaggct ggcgcccttg tcattccaga tgccttccgc
     3961 gttgcacgcc gtcacgtgaa aaacataatt gccgctcggg agcggcccgt aaccgatttg
     4021 tctttcggtt ccgttttgca cccaatcggc gtcaatccca tccatcttgt gacggaagcg
     4081 cactttttcc ggcgcatcaa aactcagtcc gacgaagtgg aattcgagcg tcttcggatt
     4141 gccggggagc atgagaacat ccgcatgggc atcgctggtt gcgagagttg gcttctcagc
     4201 ggaaaattta accggttgat tatccaccaa tagattttcg atatgaacct gcggcgcggg
     4261 tttgtcagat tcgcgtcccc gaatatcgac tccaaccgga ccgctgttca gcgcaaacca
     4321 taaacggccg ccagcagaac ggatcgcgtg cggcccgccg aatctcagcg ccttgggcgt
     4381 ggattggtcc gtctcataga ccagcttgac tttaagcggt tcgggacggg ccagcgcgcg
     4441 gttgatggaa tcgctgctga tgcggcacaa gcccctaggc gtcgcaagcc aaagattggc
     4501 ttcgtcatcc tccgtcaatc cccacacgac attatcgggc aggccgtctt gcacatccca
     4561 ggcgcgccgc acatccttgt tcacataacc caatcctcct ccatccgtgc cgatccacaa
     4621 accgccgttg gtgctttcgc ataatcccaa aatcgccttg cccgcaagcc gtggcgacaa
     4681 t
//
LOCUS       DASZMM010000319         8299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2047500, whole genome shotgun sequence.
ACCESSION   DASZMM010000319 DASZMM010000000
VERSION     DASZMM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047500"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VG754_07655"
     CDS             <1..357
                     /locus_tag="VG754_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19126.1"
                     /translation="IVALGLSGVLYAGCVSKSELKAKQRQAFLAGQKQGAIVAQANAN
                     SVWVVGNVRNPSVPWTPELTLRQALIEADVQGAGDPSQIVVLRNGGQPTSVSAQELLN
                     GFDMPLQAGDRVEVRR"
     gene            554..1642
                     /locus_tag="VG754_07660"
     CDS             554..1642
                     /locus_tag="VG754_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HWF19127.1"
                     /translation="MTLSPPLQQVDRTYVRHQGRKLSYFAGCDYFRLASHPEVLEAAA
                     TGLERFGLNVSASRMTTGNHPLYEQLERSIASFFGAPTATLASNGYAPNLMVAQALAG
                     QFSHALIDERAHGCLTDAAQLLDCPVVKFRHRDATDLARIVQRLGNISPLVLTDGMFS
                     HDGSIAPLKEYLAALPAGGMILLDDAHGAGVLGKTGRGTPELAGISTKRIIQTITLSK
                     GFGVYGGAVLGPRQLRSSILAKSRMFVGNTPLPLPLASAAIKSIAILKSDKKLRARLA
                     QNTAWLKGELKRHGLPVMETPSPIFALVPQSAKEAERVKKRLLARGIYPPFIKYPGGP
                     ESGYFRFAISSEHTAKQLDNLVKALTGR"
     gene            1857..3035
                     /gene="sppA"
                     /locus_tag="VG754_07665"
     CDS             1857..3035
                     /gene="sppA"
                     /locus_tag="VG754_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWF19128.1"
                     /translation="MDSNLPPENTPPPVFNAPPPQPPYTPPPPIIPPAPAPRPPKRGG
                     TGWKVLALILFVLLIASVALNVGKVSRKVVGGRSRTYRANSGAYGPRLEEITLKESPL
                     YTTNKIAVVPVTGIISGDAMQDSDFSLVTVIKEELQLAKKDDDVRAVILKVDSPGGEV
                     LASDEIAKAIIDFRDKSHKPVIVSMGSLAASGGYYVSAPCDWIVANELTITGSIGVIM
                     HGLNYRGLLDKIGVQPEVFKSGKFKDMLSPTKKPEEISPEEKQMVQDLITETYDKFKS
                     VVADGRKSAFRRNKGQKLSDDWTDYADGRILSGKQAYKLGFVDEIGDFDTTVDTAKRL
                     AGIGRADIVEYKPIFDLSNIFRLFGETDAKKTVKVELGVDVPKLHAGYLYFLAPTYLQ
                     "
     gene            3037..3660
                     /gene="upp"
                     /locus_tag="VG754_07670"
     CDS             3037..3660
                     /gene="upp"
                     /locus_tag="VG754_07670"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HWF19129.1"
                     /translation="MGRVTLIKHPLVQHNLTRLRDKRTGPQEFRRIMSEVAALIIYEA
                     TRSFTTQPLTVTTPLARIRGSQLQREVILVPVLRAGLGMLDSILQLIPHARVGFIGLK
                     REEKSLRALFYHKSLPRDLSRFEVILIDPMLATGGSAVSAMDLLTELGAKHVRLVNLV
                     AAPEGIRQIHTHYPRLPIFTAAIDQKLNGNGFIVPGLGDAGDRLFGV"
     gene            3719..4843
                     /locus_tag="VG754_07675"
     CDS             3719..4843
                     /locus_tag="VG754_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWF19130.1"
                     /translation="MKNLLMVADSDHDANMLYAVGMFVPDPFIYMRIRGKDLLVMSDL
                     EIDRARKQAPHCQVYSLSFYQQKLKRAGLKSPGVADVVSAVLEEHRVGKVFVPGNFPY
                     SLALELRDAGIKVKPKPNGFFPEREEKSADEVKKISAALMMAEVGLAEAIQALKAAKI
                     GKNRKLIYRNAPLTSEKLRSIIDTAIIQASGLASHTIVAGGKQACDPHESGYGPLRAN
                     ESIILDVFPRSQKTGYFGDITRTVVKGRASEAVRKLYDTVHRGQKIAFSLMRAGTETS
                     KVHKAVQELFVREGYKTGKVGGRMQGFFHGTGHGLGLEIHEAPRVGATSKGALKAGHV
                     VTVEPGLYYPSIGGVRLEDVALVTRGAPRNLTQFEKVLEV"
     gene            5202..5906
                     /locus_tag="VG754_07680"
     CDS             5202..5906
                     /locus_tag="VG754_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HWF19131.1"
                     /translation="MFFNPFYYPYWWFAIPGLLLGLYAQFRLSADYRRYSEVPIESGL
                     SGAEAAREILDRNGLNNIAIEAISGHLTDHFDPLRKALFLSEENYSGRSIAAVGVSAH
                     EAGHALQQKAAYPLFNFRMWLAPATQFASYAYMGIFFLGWILGSAMFAKFLLIGIGLF
                     AIITLFQIITLPVEYDASRRAKQQLISLGLVQPSESAAVSRMLYSAGLTYVAAMLSAV
                     LQLLRLIMIYNRTERR"
     gene            6051..7082
                     /locus_tag="VG754_07685"
     CDS             6051..7082
                     /locus_tag="VG754_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF19132.1"
                     /translation="MAAKLKRPVKINGKNRLHTSKTKAPRFSPVKAAVAPAPSPPKAE
                     AFAAPLELDLRPEPASPLVERERSSYDGDTAIKLYLREIGQVRLLTPQEEIELAARIK
                     KGDKKAREQMIKANLRLVVKIARDYEGIGLPLLDLISEGNIGLMKAVERFDPAKGGKL
                     STYGSWWIKQSIKRALANQSKTIRLPVHLVDKISKMRRTALKLQEILGREPTDEELAE
                     ELGISATRVGQMRTAAIRPASLDAPIGDEDSNNFAEVVQDENADTPYELLEEKTVVKM
                     LQEMVTTLDPREATILRARFGLDGGSQRTLEQVGEKFGVTRERVRQIQNIALKKLRKM
                     IEKFESKKT"
     gene            7119..7655
                     /locus_tag="VG754_07690"
     CDS             7119..7655
                     /locus_tag="VG754_07690"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWF19133.1"
                     /translation="MNLTQPTPADIERAIRNIPDFPKPGIQFKDITPVLADPRLFAGS
                     IDLLTAGYKPGTIDAIVGIDARGFIFAAAAALRLEAGFVPVRKKGKLPYQTHEQNYDL
                     EYGANAIAIHIDAVKPGSRVLLIDDLLATGGTAAAAAALLKKVGAQILEISFLIELTF
                     LGGRDKLKDYPLRSIVRY"
     gene            7737..>8299
                     /locus_tag="VG754_07695"
     CDS             7737..>8299
                     /locus_tag="VG754_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWF19134.1"
                     /translation="MQLLQTLDVSLFHLVNPALSSGLLDVLMPFSSGNIFFAPALLVG
                     AILLLWKGGARGRVCVAMLLLVVAFGDGVVCNTLKQVIHRPRPFWTLADVHVPHRIGK
                     TDSGSMPSSHAANWFSATMVAFIYYRRSIRFMLPMAMLVSFSRIYNGVHYPSDVVVGA
                     ILGAGYAAALVWTANETWRWAGARWFP"
BASE COUNT         1825 a         2269 c         2350 g         1855 t
ORIGIN      
        1 attgtcgcat taggtttatc gggcgtgttg tacgcgggtt gtgtttccaa atcggagctt
       61 aaggcgaagc agcggcaggc ttttttggcg gggcaaaaac agggcgcaat agtagcgcaa
      121 gcgaacgcga attcggtttg ggtcgtcggg aatgtccgca atccgtcggt tccatggacg
      181 ccggaattga cgcttcggca ggccttgatc gaagcggatg ttcagggcgc gggagatcca
      241 agccagatcg tggtgctgcg caatggcggc cagccgactt ccgtcagcgc ccaagaattg
      301 ctgaatggat tcgacatgcc gttgcaagca ggtgaccgcg tggaagtgag acggtgaaaa
      361 gctccaaacg ccaacctcca agcaccagag aatctccaaa cttcaatacc gattttccac
      421 tgttcgggac gaagcttttc atttgaaatt tcaaattagt catttgaaat ctgattccga
      481 ttcctcacgt cggctcctgc gataattttt ttaacgtgcg cttctttttg cgctggctta
      541 taccaatccg gtcatgacgc tttcaccgcc gttgcaacaa gtggaccgca cgtatgtgcg
      601 tcatcagggg cgcaaacttt cctacttcgc cggttgcgat tatttccggc ttgcgagcca
      661 tccggaagtg cttgaggccg cggcaacggg cctcgaaagg ttcggtctca acgtctcggc
      721 gtcgcggatg acgaccggca atcatccgct ttacgaacaa ttggaacgaa gcatcgcctc
      781 ttttttcggt gcgccgaccg caacgctggc atcgaatggt tacgcgccga acctgatggt
      841 tgcgcaagcg ttagccggac aattttctca cgcgttgatt gatgaacgcg cgcacggctg
      901 cctcaccgac gccgcgcaac tgctcgattg tccggtggta aaattccggc atcgggacgc
      961 gaccgatctc gcgcgcatcg tgcaacgact cggcaatatc agtccgctgg ttctcacgga
     1021 cggaatgttt tcgcacgacg gcagcatcgc cccgctcaaa gaatatctcg ccgcgcttcc
     1081 cgctggggga atgattttgt tggacgacgc gcatggcgcg ggcgtgctgg gaaaaacagg
     1141 tcgcggcaca ccggaactgg ccggcatttc caccaagcgc atcatccaga ccatcacgct
     1201 cagcaaagga ttcggtgttt atggcggagc ggtgctgggc ccgcgccagc ttcggtcatc
     1261 gattctggcc aaaagccgga tgttcgtcgg caacactccc ctgcccttgc cgcttgcaag
     1321 tgccgcgatc aaatcgatcg ccatccttaa aagcgacaaa aaattgcgcg cgcgacttgc
     1381 acaaaatacc gcctggctga aaggcgaatt gaaacgtcac ggtttgccgg tgatggaaac
     1441 acccagcccg attttcgcgc tggttccgca aagcgcgaag gaagcggagc gtgtgaaaaa
     1501 acgtttgctg gcgcggggca tttacccgcc gttcatcaaa tatccaggcg ggccggaaag
     1561 cggctatttt cggttcgcga tttcgagcga gcacaccgcg aagcaattgg acaatttggt
     1621 gaaagcgctc actggaagat aagctccaac atccagagaa attccaaaca tcgatcacgc
     1681 aatccgggga ctacgacacc attcatgttg cggcgatagc ttggacactg cgcgcgtatt
     1741 gtttggagct tgaagattgg cgcttctctg gcgcttggat gttggtgatt ggagctttgc
     1801 gcgaatggag cgttttacct cttgtccgtc cagtttgttt ggagtagctt ccccccatgg
     1861 atagtaatct gccgcctgaa aatacaccac cgccggtgtt caacgcgcct cctccgcagc
     1921 caccttatac tccgccgccg cccatcattc cgcctgcgcc cgcgccgcgc cctcccaaac
     1981 gtggtggaac aggctggaaa gttctggcct tgattctttt cgtgttgctg atcgcgagcg
     2041 ttgctttgaa tgtcggcaaa gtttcgcgga aagttgttgg cggacgatcc cggacttatc
     2101 gcgccaactc aggcgcgtat ggcccgaggc tggaggagat cacgctcaag gaaagccctc
     2161 tttacacgac caacaaaatc gccgtggtcc cggtcaccgg catcatcagc ggcgacgcga
     2221 tgcaggacag cgattttagc ctcgtgactg tgatcaagga agaattgcaa ttggcaaaga
     2281 aggacgatga cgttcgcgcg gtgatcctca aggtggattc gccgggcggc gaagttttgg
     2341 cgtcggatga aatcgcgaaa gcgatcattg attttcggga caagtcgcac aagccggtga
     2401 tcgtgagcat gggaagtttg gcggcatcgg gcggttatta tgtctccgcg ccttgcgatt
     2461 ggatcgtggc aaacgagttg accatcaccg gcagcattgg cgtcatcatg cacgggttga
     2521 attatcgcgg cttgctcgac aagattggcg tgcagccgga agttttcaaa agcggcaaat
     2581 tcaaggacat gttaagcccc accaagaagc cggaagaaat ctcgcccgag gaaaaacaga
     2641 tggtgcagga cttgatcact gagacctacg acaaattcaa aagcgttgtc gccgatggcc
     2701 ggaagtcggc tttccgccgc aacaaaggcc agaaattaag cgacgattgg accgattatg
     2761 ccgacggtcg catcctctca ggcaagcagg cttacaagct tggtttcgtg gatgaaatcg
     2821 gggatttcga cacgacggtg gacacggcca agcgtttggc gggcattggc cgcgcggaca
     2881 tcgtggaata caagccgatt ttcgaccttt ccaacatctt ccggttgttc ggtgagaccg
     2941 atgcgaaaaa aacggtgaaa gtggaactgg gtgtggacgt tccaaaattg cacgccggtt
     3001 atctttattt cctagcgcca acttacctcc aataacgtgg gccgggtcac ccttatcaag
     3061 catccgctgg tgcagcataa tctgacgcgg ttgcgcgaca aacgcacggg cccgcaggaa
     3121 tttcggcgca tcatgagcga ggtggcggcg ctcatcatct acgaagcgac gcgctccttc
     3181 accacccagc cattgactgt gacaacgccg ctggcgcgga ttcgcggttc ccaattgcaa
     3241 cgcgaggtga ttttggtgcc ggtattgcgc gcggggttgg gaatgctcga ttccatattg
     3301 caattgattc cacatgcgcg agtcgggttc atcggcttga agcgcgagga aaaaagtttg
     3361 cgcgcgcttt tttatcataa aagtttgccg cgggacctga gccggttcga agtcattttg
     3421 atcgatccaa tgctggcgac tggcggcagc gcggtttccg cgatggactt gctgacggaa
     3481 cttggggcaa aacatgtgcg gctcgtgaac cttgtggccg cgcccgaggg cattcggcag
     3541 atacacaccc attatccgcg gttgccgatt ttcaccgccg ccattgacca gaaattgaac
     3601 gggaacgggt tcatcgtgcc cggattgggc gatgccgggg acaggctttt tggggtttga
     3661 atttttttac gacttccgct aacgtcaccg ccggatttct ccaaaacgtg atactgcgat
     3721 gaaaaatctt ttgatggttg cggacagcga tcacgacgcg aacatgcttt atgccgtggg
     3781 catgttcgtg cccgatcctt tcatttacat gcggatccgg ggcaaagacc tgctcgtgat
     3841 gagcgatttg gagatcgacc gggcccgcaa gcaagcgccg cattgccagg tctattcatt
     3901 gagcttctat cagcaaaaat tgaagcgcgc agggttgaaa tctccggggg ttgcggatgt
     3961 cgtgagcgcc gtccttgaag aacatcgcgt gggcaaagtt ttcgtgcctg gcaattttcc
     4021 gtattcgctg gcgctggaat tgcgcgacgc gggcatcaag gtgaagccca agcccaatgg
     4081 atttttcccc gaacgcgagg aaaaatcagc cgatgaagtg aaaaaaatca gcgccgcgtt
     4141 gatgatggcg gaagtcggtt tggccgaggc gatacaggcg ttgaaagccg ccaagatcgg
     4201 gaagaaccga aagttgatct atcgcaacgc tcccctgact tccgagaaat tgcgttccat
     4261 cattgacacg gccatcatcc aggcgagcgg gttggcgagc cataccattg tggccggcgg
     4321 caaacaggct tgcgacccgc atgaatcggg atacggtccg ttgcgcgcga atgaatcgat
     4381 cattcttgat gtttttccac gctcgcaaaa gaccggttat tttggcgaca tcacccgcac
     4441 ggtggtcaaa ggccgcgcca gcgaagccgt ccgcaagtta tacgacaccg tccatcgcgg
     4501 gcaaaaaatt gccttctcgc tcatgcgcgc cgggacagag acgtccaagg tccacaaagc
     4561 cgtccaggag ttgttcgtgc gcgaaggtta caagaccgga aaggtcggcg gccggatgca
     4621 gggatttttc cacggcacgg gacacggcct tggcttggaa atccatgaag cgccgcgtgt
     4681 gggcgcgact tcaaagggcg cgttgaaagc cgggcacgtt gtcactgtcg agcctggact
     4741 ctattatccg agcattggcg gagtgcgttt ggaagacgtg gcgcttgtca cccgcggcgc
     4801 gccgcgaaat cttacgcagt tcgaaaaagt gctcgaagtg taacggtcac cctgacttcg
     4861 ccgggacggc tcagacgcca cacacgacgc gtgggctccc gattccctaa atataattac
     4921 ggctcagaca aacctgaaac atacgggcag gtgcgacaca aaggcgcatc aaagccgctc
     4981 aaacagcgtc aatttatcgc gttttaactc cattttttcg cagtcgtttt gtgaaacgtc
     5041 tccccatcaa tgtcttgcga cattggcatc aaatccgcta aagagttgaa catgggtgtg
     5101 caagctgtcg tcaattggtt aagtagatgt tgtgaatttt gagaaaggtt tcgcgcgaat
     5161 ggttttcagg ttaggttgtt ttgtcggaaa aaagtaaagc catgtttttc aatccatttt
     5221 attatccgta ttggtggttc gccatcccgg gtttgctgtt ggggctgtat gcgcaattca
     5281 ggttgtcagc ggattacagg cgttattccg aagtgccgat tgaaagcgga ttgtccggcg
     5341 cagaagccgc gcgcgaaatc ctcgaccgga acggactgaa caatatcgcc atcgaggcaa
     5401 tcagtgggca tctgaccgac cattttgatc ctctccgcaa agcgctgttc ctctccgaag
     5461 aaaattactc gggacgctcg attgcggcgg tgggcgtctc cgcgcacgaa gccggccatg
     5521 ccctgcaaca aaaagccgcc tatcccctct tcaacttccg catgtggctt gcgccggcga
     5581 cgcaatttgc cagttacgcc tatatgggca tctttttcct gggctggatt ttgggcagcg
     5641 cgatgttcgc aaaatttttg ctcatcggca tcgggctttt tgcgatcatc accctcttcc
     5701 agatcatcac gttgccggtg gaatacgacg ccagccgacg ggccaagcaa cagctaatca
     5761 gtttggggct tgtgcagccg tcagaaagcg ccgccgtgag ccggatgctc tattcggccg
     5821 gattgactta tgtggcggcc atgctctcag ccgtgttgca attgctcagg ttaatcatga
     5881 tttataaccg caccgaacgg cgctgactgt tgtttaatca ggtgaacagc atgggcataa
     5941 cgccctattt atggccaact tcacttttgc tatggataag cggcggcatg gagcaggtgc
     6001 ttatttgctt gcgaagaagg tggtaattcc tgatgtatga aatcgttttt atggctgcca
     6061 aactgaaacg cccggttaaa atcaacggca aaaaccggct gcacacctca aaaaccaagg
     6121 ccccgcgatt ttccccggtg aaagcagcgg tggcccctgc cccaagtccg cccaaagccg
     6181 aagccttcgc cgccccattg gaattggatt tgcgcccgga accggcgtcg cccttggtgg
     6241 agcgcgagcg ttcctcttat gacggcgaca ctgccatcaa gctttacctg cgcgaaattg
     6301 ggcaggtgag attgctgacg ccccaggagg aaattgagtt ggccgcccgc atcaagaaag
     6361 gcgacaagaa agcgcgcgaa cagatgatca aggcgaacct tcgcctggtg gtcaaaatcg
     6421 cccgcgatta tgaaggcatc ggtttgccgc tgctggacct catcagcgaa ggcaacatcg
     6481 ggttgatgaa ggcggtggaa cggttcgatc ccgccaaagg cggcaagctt tccacgtatg
     6541 gttcgtggtg gatcaagcaa tccatcaagc gcgcgctcgc caatcaatcc aagaccatcc
     6601 gcctgcccgt tcatctggtg gacaaaattt ccaagatgcg ccgcaccgca ttgaagctgc
     6661 aagaaatcct cgggcgcgaa ccgacggacg aggagttggc ggaggaattg ggcatttccg
     6721 ccacgcgcgt cggacagatg cgcaccgcgg ccatccgccc cgcttcgctg gacgcgccca
     6781 ttggtgatga ggattccaat aattttgccg aggtggttca agacgaaaac gccgacacac
     6841 cttacgagct gcttgaagaa aaaaccgtcg tcaagatgtt gcaggaaatg gtcaccacgc
     6901 tcgacccgcg agaagcgacg atcttgcgcg cgcgattcgg cctggacggc ggcagccagc
     6961 ggacgcttga gcaagtgggc gaaaaattcg gcgtgacgcg cgaacgcgtg cgccagatac
     7021 agaacatcgc cttgaaaaag ctccgcaaga tgatcgagaa gtttgaatcc aaaaaaactt
     7081 gatacccgca tccttcgccg ccacgatatg tctcaagcat gaatttgaca caaccgacgc
     7141 ctgcggacat cgaacgcgcg attcgcaaca tccccgattt tccaaaacca ggcatccagt
     7201 tcaaggacat cactcccgtt cttgccgatc cgcggctttt tgcgggcagc attgatttgc
     7261 tcaccgccgg ttacaagccc ggcacgatcg atgccattgt cggaattgac gcgcgcgggt
     7321 tcatcttcgc cgccgcggcg gcattgcggc tcgaagcggg tttcgttccc gttcgcaaaa
     7381 aagggaaatt gccctatcaa acccacgaac agaattacga cctcgaatac ggcgcgaacg
     7441 caatcgccat ccacatcgac gccgtcaagc ccggaagccg cgtgctcttg attgacgatc
     7501 tgctggccac cggcggcacc gccgctgcgg ccgctgcgtt gctcaaaaaa gtcggcgcgc
     7561 aaatccttga aatctccttc ctcatcgaac tcacctttct tggtggtcgc gacaaactca
     7621 aggattatcc cctccgctcc atcgtccgct attaaatgga tttggtttgc gcccgcgatt
     7681 tcctggccaa actttattgc tttgcaactc gccgcagcaa cgtaaactgt ggttgaatgc
     7741 agttgttgca aacacttgat gtcagcctgt ttcacttggt caaccccgcg ttgagcagcg
     7801 ggttgctgga cgtgctgatg ccgttttcca gcggcaacat tttcttcgcg cccgcgttgc
     7861 tcgttggcgc gattttgctg ctgtggaaag gcggcgcgcg cggacgggtt tgcgtggcga
     7921 tgttgttgct ggttgtcgcc ttcggtgatg gcgtggtctg caataccctc aagcaagtca
     7981 ttcatcggcc gcgtccgttt tggacgttgg cggatgttca cgtccctcat cgcatcggca
     8041 agactgattc cggcagcatg ccgtcctccc acgccgcgaa ctggttttca gccacgatgg
     8101 tcgccttcat ttattatcgg cgaagcatcc gtttcatgct gccgatggcg atgcttgtca
     8161 gcttttcgcg catctataac ggcgtgcatt atcccagcga tgtcgtggtc ggagcgattc
     8221 tcggcgccgg ctatgccgca gctttggttt ggacggcgaa tgaaacctgg cgatgggctg
     8281 gagcgcgctg gtttccgat
//
LOCUS       DASZMM010000320         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3981298, whole genome shotgun sequence.
ACCESSION   DASZMM010000320 DASZMM010000000
VERSION     DASZMM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981298"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VG754_07700"
     CDS             complement(<1..479)
                     /locus_tag="VG754_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19135.1"
                     /translation="MQRVASSSDDQNRDRMVCALLALVTLLLYWPTRHFEFNNYDDAQ
                     YLTTNPHVQSGLTWSAIKWAFTTGYAANWHPLTWLSHALDYQIYGSNAGGHHLTGVLF
                     HIADSVLLFLLLRQLTGAFWRCAIVAALFAWHPLHVESVAWVAERKDVLSMFFGLLT"
     gene            598..1323
                     /gene="pyrH"
                     /locus_tag="VG754_07705"
     CDS             598..1323
                     /gene="pyrH"
                     /locus_tag="VG754_07705"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWF19136.1"
                     /translation="MKKSDKSRYRRILLKLSGEALGGPTGLGISPEAVQDMAQQIMEV
                     RELGVEVVVVIGGGNIFRGLQGSERGIERATGDYMGMLATIINSLALQDALEKLGVAT
                     RVQTAITMAQVAETFIRRRAVRHLEKGRVVIFGGGTGNPYFSTDTAAALRANEIGAEV
                     ILKATKVDGIYDCDPKKNPDAKRYVQITYLQALQQQLKVMDSTAFSLCMDNKMPIVVF
                     DFFRPHNLRRVVMGEKVGTLVTA"
     gene            1442..2074
                     /locus_tag="VG754_07710"
     CDS             1442..2074
                     /locus_tag="VG754_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWF19137.1"
                     /translation="MTFESREEAGWQLGRRLAAQGVEADIVLGLPRGGVVVAAEIARV
                     LGKPLGVLIVRKIGHPRHREYAVGAMAEEGVVLLDRAAIAETHVHQAELNEIIEEESA
                     RLRSYKERFHHEGELSLAGKNVAIVDDGLATGATAEAAVLSAKKQKARKVIVAVPVAS
                     PDGIRRLGVVADDVIALVVDPDFVAVGQYYQYFPQTTDEEVQSLLHPAHA"
     gene            2067..2924
                     /gene="mutM"
                     /locus_tag="VG754_07715"
     CDS             2067..2924
                     /gene="mutM"
                     /locus_tag="VG754_07715"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWF19138.1"
                     /translation="MPELPEVEVLARHLAPLLKGRTIGNISVRRARVIAPTSEPELAD
                     ALKGATFTELNRRGKYLLFTLRQEKKSEPFTLVGHLGMTGRIYLAPKSASLPKHAAVV
                     LDLGSENFIFEDTRYFGRLTLDTCGIEKLGPEPLGVKFTVDYLASALKRSAQSVKVKL
                     LDQTLVAGVGNIYASEALFRAAISPRMASRKLNLGQVKSLHRAIPEVLTRAIRFGGSV
                     PLDFAGNTGRSDGLFYYGSGEESSEDCPERFLVYDRVGKPCLECGKAIRRIVQGARST
                     FYCPHCQHP"
     gene            2986..3060
                     /locus_tag="VG754_07720"
     tRNA            2986..3060
                     /locus_tag="VG754_07720"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3018..3020,aa:Asn,seq:gtt)
     gene            complement(3225..3926)
                     /locus_tag="VG754_07725"
     CDS             complement(3225..3926)
                     /locus_tag="VG754_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF19139.1"
                     /translation="MNNTIKKKVTVLIIDDEVHMRRMLQVYLERNEYEVKVAATGDEG
                     INKVIEFQPDVILLDLGLPDMDGLEVLKRLREWTQTPIFVVSVRAREEEKIAALDKGA
                     NDYLTKPFGTGELLARLRVVRRYGQTQSATEIFKSGHLEVDLASRLVKVKGRAIKLSP
                     TEYSLLALFVQHAGKLLTQDYLLREIWGSSDPARMGALRVYIRYLREKIETDPAMPEI
                     LITESGIGYRLIAQD"
     gene            complement(3923..>4507)
                     /locus_tag="VG754_07730"
     CDS             complement(3923..>4507)
                     /locus_tag="VG754_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19140.1"
                     /translation="RDIVAAIHDATERLNRLVGNVLDITRLESGHIKARFSESSVGDL
                     VHLALTNTKVQLAKHKVSVEIAPDLHTVSMDFVLMEQALTNLLSNAAFHTPAGTEVKV
                     IVRMERETLIIEVSDRGPGIPSQSIGRLFDRFYRGPSAPTGGSGLGLSLVKGFVEAQG
                     GAVHANNGSGGGAIFSIHLPLAKVPVVPVEPMHA"
BASE COUNT         1060 a         1195 c         1246 g         1006 t
ORIGIN      
        1 aacgtcagca gaccgaaaaa catgctcaag acatctttgc gttcggccac ccacgcgacg
       61 gattccacat gcaacggatg ccacgcgaac aatgccgcga caatagcgca tcgccagaac
      121 gctcctgtca attgccgcag cagcaggaaa agcagcacgc tgtccgcgat gtgaaacaaa
      181 acgcccgtca gatgatgacc gccggcattc gagccataaa tttggtaatc caacgcgtgc
      241 gaaagccagg tcagcggatg ccagttcgcg gcgtaacctg tcgtgaaagc ccacttgatg
      301 gcgctccatg taagcccgga ttgcacgtgc ggattggtgg tgagatattg ggcgtcatcg
      361 taattgttga actcaaagtg acgcgtcggc caatagagca acaaggtcac cagcgcgagc
      421 aaagcgcaga ccatccggtc ccggttttgg tcatcgcttg aagaggcaac gcgctgcatg
      481 gcagaagcgt aagcaaagac acctcgttga acgaagaaaa aactgacaaa gcgcatttga
      541 cgcgcgggga tgcgattgtt acaaacactg ggcgttcgca aattgtcaaa cagcggcatg
      601 aagaaatccg acaaatctcg gtatcgcagg atcctgctca agctcagcgg tgaagctttg
      661 ggcgggccga cgggccttgg catcagtccc gaagccgtgc aggacatggc gcagcagatc
      721 atggaagtgc gcgaattggg cgtggaagtg gtggtggtga tcggcggcgg aaatattttt
      781 cgcggtcttc aaggcagcga acgcggcatc gaacgcgcca ccggcgatta catgggaatg
      841 cttgcgacga tcatcaattc gctcgcgttg caggacgcgc tggaaaaatt gggagtggca
      901 acgcgcgtgc aaaccgccat cacgatggcg caagtcgcgg agacattcat ccgccgccgc
      961 gccgtgcggc atctcgaaaa gggcagggtg gtcatctttg gcggcggcac gggcaatcct
     1021 tacttttcaa ctgatacagc cgccgctttg cgcgcgaatg aaatcggagc ggaagtcatt
     1081 ttgaaggcca cgaaagtgga tggcatttac gactgcgatc ccaagaaaaa tccagatgcc
     1141 aaacgctacg tgcagatcac gtatcttcag gccctgcagc aacagctcaa ggtgatggat
     1201 tcaaccgcgt tctcgctttg catggacaac aaaatgccga ttgttgtttt cgattttttc
     1261 cgcccacaca accttcgccg cgtggtgatg ggcgaaaaag tcggcacgct cgtgaccgct
     1321 tgaactgaat tgccgcaaac tgaaaagcca tagccgttct tcactctcca ctgttatccc
     1381 gcgccctttg ccgccgtttg cggctattga ttttcgagca aaatggggcg atttttagga
     1441 gatgactttt gaatcgcgag aggaagcagg atggcaactc gggcggcggt tggcggctca
     1501 gggagttgag gcggatattg ttctcggctt gccgcgcggc ggggtggttg tggcggcgga
     1561 aattgcacgt gttttgggga agccgctggg ggttttgatt gtgcgaaaaa ttgggcatcc
     1621 ccgtcatcgc gaatatgcag ttggcgcgat ggcagaagaa ggtgtggtgt tgcttgaccg
     1681 cgccgcaatc gcagaaaccc atgtgcacca ggcggaattg aacgaaataa tcgaggagga
     1741 gtccgcgcgg ctccgcagtt acaaggagcg cttccaccat gaaggcgaac tatctctggc
     1801 cggcaaaaac gtcgccattg ttgacgacgg gcttgcaacc ggcgcaacag ccgaagcggc
     1861 ggtgctttcg gccaagaagc aaaaagcgcg caaagtgatc gttgcagttc ccgtggcatc
     1921 gccagatggt ataaggcgcc tcggcgtagt cgcggatgat gtcatcgcat tggttgtgga
     1981 tcctgatttc gttgctgtcg gccaatatta tcagtatttt ccccaaacaa ccgacgagga
     2041 agtccagtcg ctgctccatc ccgctcatgc ctgaactgcc cgaagtcgaa gtccttgccc
     2101 gccatcttgc gcccttgctg aaaggcagga cgatcggaaa tatttctgtg cgccgagcga
     2161 gagtcatcgc tccaacttcc gaacccgaat tggccgatgc attgaagggc gcgactttca
     2221 cggaattaaa tcggcgcggc aaatatttgc tgttcacact ccgccaggag aaaaagtccg
     2281 aaccattcac actcgtgggg catctcggaa tgaccggccg tatttacctt gcgcccaagt
     2341 ccgcatcatt gcccaaacac gccgccgtgg tgctcgacct cggcagcgaa aatttcatct
     2401 tcgaggacac gcgttatttt ggacgactga cgctcgacac atgcggcatt gaaaagctcg
     2461 gtccggagcc gttgggcgta aaattcactg tggattacct tgcatcggca ttgaagcgtt
     2521 ccgcgcaaag cgtcaaagtc aaattgctcg accagacgtt agtggcgggc gtcggaaata
     2581 tttatgcaag cgaggcattg tttcgcgctg ccatttcccc gagaatggct tcccgaaaat
     2641 tgaacttggg acaggtcaaa agcctgcacc gggccattcc ggaagtcctg acgcgggcga
     2701 tccgtttcgg cgggagcgtg ccattggatt ttgccggaaa cacgggacgc agcgatgggc
     2761 ttttctatta cggcagcggg gaggaatcgt cggaagattg cccggagcga tttttagttt
     2821 atgatcgggt tggcaagccg tgtttggaat gcggcaaagc gattcggcgc attgtgcaag
     2881 gcgcgcgcag cacattttac tgcccgcatt gccagcatcc gtgagatggg gtaaaatcag
     2941 ttgacgcagg catgtgtggc cggtaaattt ctcgtccctt tggcatccaa agtagctcaa
     3001 tggtagagca atcggctgtt aaccgatcgg ttgtaggttc gagtcctacc tttggagcca
     3061 cttcttgttg atgttcaacg gcagctccac aaacaggtgc gaaaagtgcc atttgccaga
     3121 agaactgtca attcggtata ggattcatct ttaaatcaaa aagagcaatt ttccgcgagg
     3181 taatcatgcc atttctcttt taaggcgatt ggaggccaag gttctcaatc ttgagcaatt
     3241 aaacgatagc caatgcccga ttcggtgatc aaaatctccg gcatggccgg atcggtttcg
     3301 attttctcgc gcagataacg aatatatacc cgcagcgcgc ccattcgcgc gggatcggat
     3361 gaaccccaga tttcccggag aagatagtct tgagtcaaaa gttttccggc atgttgaaca
     3421 aacaaagcca atagtgagta ttccgtaggt gacaatttta tcgccctccc ttttaccttc
     3481 accaagcggc tggccagatc aacctccaga tgaccggact tgaaaatctc cgtggctgat
     3541 tgggtctggc catagcgcct gacgacgcga agccgcgcga gcaactcgcc tgtgccgaaa
     3601 ggtttggtaa gataatcatt tgcgcccttg tccaacgcgg caattttttc ttcttctcgg
     3661 gcccgcaccg aaaccacaaa aatcggtgtt tgagtccatt cgcgcagtcg tttcaagact
     3721 tccaacccgt ccatgtccgg caatccaaga tcaagcagga tgacatccgg ctggaactcg
     3781 atcaccttat tgatgccttc atcgccggtc gcagccactt tgacttcgta ttcgttacgc
     3841 tcgaggtaca cttgaagcat acgtcgcatg tggacttcat catcaatgat caggactgtt
     3901 accttttttt taatcgtgtt gttcatgcgt gcattggttc aactggaact acaggaactt
     3961 ttgcaagcgg caaatgaata ctgaagatgg ccccgcctcc ggaaccattg ttggcgtgaa
     4021 ccgccccccc ctgtgcctcg acaaatcctt tcacgagcga caaacccaat cccgagccgc
     4081 cggtcggggc tgacggcccc cggtaaaatc tgtcaaaaag gcgtccgatg gattgcgatg
     4141 gaatccccgg tccccgatcc gacacttcga tgatgagagt ttcccgttcc atgcgcacga
     4201 ttacttttac ctcggttccg gccggggtat gaaacgccgc gttggacaat aagttggtaa
     4261 gcgcctgctc catcaagaca aaatccatgc tcactgtgtg caagtctggc gcaatttcca
     4321 cactcacttt atgtttggca agctggacct tggtgttggt cagcgcgagg tgaaccagat
     4381 ccccgacaga ggattcgctg aatctagcct tgatgtgccc cgattccagc cgcgtgatgt
     4441 cgagcacatt tcccaccagc cggtttaatc gttcagtcgc gtcatgaatg gcggcaacga
     4501 tgtcccg
//
LOCUS       DASZMM010000321         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3688174, whole genome shotgun sequence.
ACCESSION   DASZMM010000321 DASZMM010000000
VERSION     DASZMM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688174"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..575
                     /locus_tag="VG754_07735"
     CDS             138..575
                     /locus_tag="VG754_07735"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HWF19141.1"
                     /translation="MNPLEEKALAALPHGPEFRFLDRLVSLEPGKMGLGEYLVRGDEP
                     FLRGHFPGQPIFPGVLIIEAAAQLAGTVAQSDPQIAPLARMKLTALRNVKILGTATPG
                     QIIQIQAHITGRLGNLVQARASASVNGHAILQAELTLSGEVGK"
     gene            complement(682..1935)
                     /locus_tag="VG754_07740"
     CDS             complement(682..1935)
                     /locus_tag="VG754_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HWF19142.1"
                     /translation="MLYERWRQIADELRGETALHDVTRGERWTFAQLAAAGEKDIGAG
                     RIVFPQGKEFVFTLLRAWHSGRTVCPLEPGQKPPHFESLPPACAHLKTTSATGGVARV
                     VAFTEEQLAADADNIVATMGLRRDWPNLGVISIAHSYGFSNLALPLLLHGIPLVLLDL
                     PLPEMVRRAAENFEAITLPAVPALWRVWHEANAIPKNVRIAISAGAPLPLMLEQSVFA
                     TNGLKLHNFYGSSECGGIAYDTSPTPRNDAACAGSPMKNVQLSVGADGCLEVRGRAAG
                     QTYWPEAAANLTNGCFRSSDLAEIRDGLVFLRGRAGDQINVAGRKLSPEEIEKALLSH
                     PEVRDCIVFGVPADDAERADIIVACVASNGSLTGDTLRSFLLERLPAWQIPREWVFVP
                     SLEMNQRGKISRQEWRSRYLAQRKD"
     gene            1979..2830
                     /locus_tag="VG754_07745"
     CDS             1979..2830
                     /locus_tag="VG754_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19143.1"
                     /translation="MRVEAPDRPKPGHQTVFWPAYFFRLTFGFLILGFLLCGCATSRP
                     LPQNTKTFNFQTDTFGFPNQLVWEYHFDEHGKWVSHPREPAPDYSHRCFVVARSTRQF
                     FEHARFDPSQPVADTETYRQVIRRVLSTSPQHLLSDSNRIVIPGYADLHSFSLAQDKL
                     LKYECGGAWQSYVQHGHWRMLLPFSRHHQEKMAAQLEAAVKANHPPVAHLVRFPQLTI
                     NHAIVLFDVKETDTAVLFTAYDPNNPKSPVTLTFDRAKRTFQFPANDYFPGGRVDVYE
                     VYRDWRY"
     gene            complement(2982..>3404)
                     /locus_tag="VG754_07750"
     CDS             complement(2982..>3404)
                     /locus_tag="VG754_07750"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HWF19144.1"
                     /translation="WLIKSRFGFNDQRMSQKFHKHYSREEARALLPQLRKWLEQLASL
                     NVEFEKNERRLAGLLASGQDTGGSLVNTSVKLQGEIRWLFREFQEREIFIKDLDRGLI
                     DFPAIVGGKEVFLCWEKDEDDIEYWHDIDSGFASREPL"
BASE COUNT          771 a          982 c          886 g          765 t
ORIGIN      
        1 accgttttgc aatttgcgga tggctcgacc atggagaata ttttcgccaa cgcccagatg
       61 aacccgaaga ttgacgaatc ccttttcaat cccgatgtga ccggctataa gataagcgag
      121 ccattcaaaa aatagcgatg aacccgctcg aagaaaaagc gctggctgcg ttgccgcacg
      181 gaccggagtt ccggtttctg gatcggctcg tgagtcttga gccgggcaag atgggcctgg
      241 gcgaatacct ggttcgcggc gacgaaccgt ttctgcgcgg ccattttccc ggccagccga
      301 tttttcccgg cgtattgatc atcgaagcgg ccgcgcaact cgccgggact gtcgcccaaa
      361 gcgatccaca gattgcgccg ctcgctcgaa tgaaactgac ggcgttgcgc aatgtgaaaa
      421 ttctcggcac ggcaacgccg ggacaaatca tccagatcca ggcgcacatc accgggcgtc
      481 tcggcaatct tgttcaggca cgcgccagcg caagcgtgaa cggccacgcc attttgcagg
      541 cggagttgac gttatcaggc gaggttggga aataaaagca ccaagcacca gagaaattca
      601 atcaccaatt ctgctgaaag tacggtgttt gatgtttgaa gttttggtgt ttccatggag
      661 tttggagctt aattgtgtaa atcaatcctt gcgttgagca aggtacctac tgcgccattc
      721 ctgtcgtgaa atttttccgc gctggttcat ttccaatgat ggcacaaaaa cccattcgcg
      781 cggtatctgc cacgcaggca atcgttccag caaaaaactc ctgagcgtgt cgccggtcaa
      841 cgagccgttc gacgccacgc aagccactat gatgtccgcg cgttcagcat catcggctgg
      901 cacgccgaaa acgatgcagt cacggacttc tggatgcgac agcaacgctt tttcaatctc
      961 ttccggcgaa agtttgcggc ccgcgacatt gatttggtcc cccgcccggc cgcgcagaaa
     1021 aaccagcccg tcgcgaattt ccgccaaatc gctgctgcga aaacagccgt tggtcaaatt
     1081 cgcagccgct tccggccaat aggtttgtcc cgccgcgcga ccgcgaactt caagacaacc
     1141 gtccgcgccg accgacaact ggacattttt cattggcgac ccggcgcaag cagcatcgtt
     1201 gcgcggcgta ggcgaagtgt cgtatgcgat tccgccgcat tcgctggaac cgtaaaaatt
     1261 gtggagcttc aatccgttcg tggcaaaaac ggattgttcc aacatcaatg gcagcggagc
     1321 gcccgcagag atggcgatcc ggacattttt tgggattgca ttcgcctcat gccacacgcg
     1381 ccagagcgcc ggcacggcag gcaatgtgat tgcttcgaag ttttcagccg cgcgccgcac
     1441 catttccggc aagggcaaat cgagcaggac aagcgggatg ccgtgcaaca gcagcggcag
     1501 cgcgagattt gaaaatccgt aggaatgcgc gatggaaatc acgccgagat tgggccaatc
     1561 gcgacgaagc cccatggtcg ccacgatgtt gtcagcgtcc gccgcgagtt gttcttccgt
     1621 gaacgcgacc acccgcgcaa cgccgccggt tgccgaggtg gtcttgagat gcgcgcacgc
     1681 tggaggcaaa gattcgaaat gcggcggctt ttggcccggt tccaatggac aaacagtccg
     1741 acccgaatgc catgctcgca ataaagtgaa aacaaattcc ttaccttgcg gaaaaacgat
     1801 gcggcccgcg ccgatgtcct tttcaccggc agcagcgagt tgggcgaacg tccagcgttc
     1861 gccgcgcgtc acatcgtgca atgcggtttc gccgcgaagt tcatcggcaa tttgccgcca
     1921 gcgttcgtaa agcattgtca gtttggtaac gcacagctac gttgcccgca agcaagttat
     1981 gcgagttgaa gcacctgacc ggccaaagcc gggacaccaa acagtttttt ggccggcata
     2041 ttttttccgg ctcacgttcg gctttttgat acttggtttc ttgttgtgcg gctgcgcgac
     2101 gagtcgccct cttccgcaaa acacaaagac tttcaacttt caaacagata ccttcggttt
     2161 ccccaaccaa ctggtctggg aatatcattt tgacgagcat ggaaaatggg tgagccatcc
     2221 gcgcgaaccg gcgccggatt actcccatcg ttgttttgtc gtggcgcgct ccacccgtca
     2281 atttttcgaa cacgcgcgat tcgatccctc gcaaccggtc gcggatacgg aaacttatcg
     2341 gcaggtgatc cgacgcgtgc tttccacaag cccgcaacat ctcttgagcg attccaacag
     2401 gattgtcatt ccgggttacg cggatttgca ttcgttcagc ctcgcgcaag acaagttgct
     2461 gaaatatgaa tgcggcggcg catggcaaag ctacgtgcag cacggccatt ggcggatgct
     2521 ccttccgttc agccggcatc atcaggagaa aatggcggct caattggaag cggcagtgaa
     2581 ggcaaaccat ccgccggttg cgcacctggt ccgttttccg caactgacca tcaatcacgc
     2641 catcgtgctt ttcgacgtca aggaaaccga cacagccgtt cttttcaccg catacgatcc
     2701 gaacaatccc aaaagtccgg tgacactcac cttcgaccgc gccaagcgca cgtttcaatt
     2761 tcccgccaac gattattttc ccggcggccg agtggatgtt tatgaagttt atcgcgattg
     2821 gcgctactga tttagagcaa aattacttaa ccacggatga acacagatga acacggatta
     2881 aatgccatac attttgcagc ttgttacgat ttcgttctta agctcccttt cgaattcacg
     2941 tttctcatcc gtgtttatcc gtgtccatcc gtggttaaaa ctcacagcgg ctcgcggctg
     3001 gcgaagccgg aatcgatgtc gtgccagtac tcgatgtcgt cctcgtcctt ttcccagcac
     3061 aaaaaaactt ccttgccgcc gacgattgcg ggaaaatcaa tcagcccgcg gtccaaatcc
     3121 ttgatgaaaa tctcccgctc ctgaaattcc ctgaacagcc accggatttc gccttgaagt
     3181 ttcacggatg tgttgaccag gctgccgccg gtgtcctgac ccgatgccag caatcccgca
     3241 agacgccgtt cgtttttttc gaactcgacg ttcaacgagg ccagttgttc aagccacttg
     3301 cgcaactggg gcaatagcgc gcgcgcttct tcacggctgt agtgtttgtg aaatttttgg
     3361 ctcatgcgct ggtcgttgaa tccaaaacga ctcttgatca acca
//
LOCUS       DASZMM010000322         6363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4522848, whole genome shotgun sequence.
ACCESSION   DASZMM010000322 DASZMM010000000
VERSION     DASZMM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4522848"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VG754_07755"
     CDS             complement(<1..228)
                     /locus_tag="VG754_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWF19145.1"
                     /translation="MEQSSLPENTTCCIAGGGPAGVMLGFLLARAGVDVLVLEKHKDF
                     FRDFRGDTIHPSTLELMYELGLLEEFLAQKHQ"
     gene            487..1485
                     /locus_tag="VG754_07760"
     CDS             487..1485
                     /locus_tag="VG754_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF19146.1"
                     /translation="MQKRKLGKSNLEVSAIGLGCMGMSFSYGPPKDKHEMIKLIHAAV
                     ERGITFFDTAEVYGPFTNEELVGEALEPYRSQVVIATKFGFDLSGSDTRPGAAGLNSR
                     PEHIKQAVEGSLKRLRVGTIDLLYQHRVDPNVPIEDVAGTVKALIQQGKVKHFGLSEA
                     GVQTIRRAHKVQPVTALQNEYSLWWRRPDAEVIPTLEELGIGLVAYSPLGKGFLTGKI
                     DENARFDSSDFRSTLPRFTPDALKTNQALIDLLEKIAQRKKATPAQIALAWLLAQKPW
                     IVPIPGTTKPGRLEENIKAVEIKLTPEDLREIESAASKIKVEGARYPEKLEQMTNR"
     gene            1549..1944
                     /locus_tag="VG754_07765"
     CDS             1549..1944
                     /locus_tag="VG754_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003569124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF19147.1"
                     /translation="MEIKRCGTQPSNKGPADWFTGTVRIDPLFQANAPARAVGASVTF
                     EPGARTAWHTHPLGQTLIVTAGCGRAQRWGGPIEEIRPGDVIWFPPGEKHWHGAASTT
                     AMTHTAIQEQLDGKTVDWLEKVTDEQYQS"
     gene            complement(2009..2170)
                     /locus_tag="VG754_07770"
     CDS             complement(2009..2170)
                     /locus_tag="VG754_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF19148.1"
                     /translation="MKRWALITGASQDIGLEFAKLFAANGWNMALIARDKPRLEKIAG
                     ELRASYGTK"
     gene            2319..2489
                     /locus_tag="VG754_07775"
     CDS             2319..2489
                     /locus_tag="VG754_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HWF19149.1"
                     /translation="MLRWAAIFLIIAIIAALFGFGGIAGAAAGIAKTLFFIFIAICVI
                     LFLVGVFAGRNV"
     gene            complement(2591..4300)
                     /locus_tag="VG754_07780"
     CDS             complement(2591..4300)
                     /locus_tag="VG754_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF19150.1"
                     /translation="MKKRSRRFWNLIALTIAIAGLASAAAVYYFTRPVAHDISHAQLE
                     LLMGGKLIHQGHIVPTPYAGIFHVQGTWQSGGKEQPFSITTHLDPEQLKSLTAQPRVV
                     IELPGGGLKEQWVNIFSALVLIGLVVGLVAFQTGLGRGQHSRIRQRPTVRFSDVAGVE
                     EAKAEIQEVVDFLRDPAKYRKLGGKLPKGVLLIGPPGTGKTLLAKAIAGEAQANFFSA
                     HGSDFNEVFVGVGAKRVRSLFKQAAKHKPAIIFIDEIDCLGKNRKLDTNSELQQTNNA
                     LLAAMDGFESSEGIVVVAATNRPEDLDEALMRPGRFDRKVQVPYPDVKGRRAILQAHA
                     WQKPIAEESALEIIAQTTPGMSGADLANLLNEAAILCAQQNREQIFLADLEASRDKVR
                     FGQERKSMVLSQKERELIAYHEAGHALINLKKTLLPPLYKVSIIPRGQALGTTTLLPK
                     EDQNIHSRDMLLQQLTVLMGGRAAEKTFLGATTNGASGDLEMARQLARKMVLEWGMGE
                     KLYYEPQQREAEIEINRILAAADAEALAMIEAQKEDAERVAQALLARETLTREEVLEL
                     VAG"
     gene            complement(4297..4509)
                     /locus_tag="VG754_07785"
     CDS             complement(4297..4509)
                     /locus_tag="VG754_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19151.1"
                     /translation="MAKAVSTLVSITLLVVAHRAAQYWHRTHARVEMAQLNARWAEAY
                     ADSTTPQIIPTNITLETCASLIPLQP"
     gene            complement(4669..5862)
                     /locus_tag="VG754_07790"
     CDS             complement(4669..5862)
                     /locus_tag="VG754_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HWF19152.1"
                     /translation="MSNDAQSLFKKHFGSPAVHLIRAPGRLELLGNHTDYNEGIVLSL
                     AVDKYVHIAAAPRTDGKIELISTAFPKPEIFWADQIKKNPEAHWADYVKGVLDQLRKR
                     GVNISGFNAAIDGTIPMGAGMSSSAALEVATALTVRQLFPYSLGETGIAKPPARDGRG
                     HLPGLTRVERMETAKICRAAESDFVGVRVGLLDQISSLFGKAWHVMEIDFRSLTVEIL
                     PLTGEAIIVCNSGVKHALIEGTYNELRRLCESAAHKLGFETLRSVDPKMLAANRAKLT
                     EREFECAHHVTSEIQRVIFASRALREDDHRQFGQYMFQSHESSRDFLKNSVPELDMLV
                     ELARAHPGCLGARLTGGGFGGATINLVAHHQAEDFMGAMARQYGERTGHKMQPILCQI
                     VDGAA"
     gene            complement(5962..6345)
                     /locus_tag="VG754_07795"
     CDS             complement(5962..6345)
                     /locus_tag="VG754_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19153.1"
                     /translation="MKIFSAGKTVKRSAAMNCLLVNQFATPGLGSIMGRRFLAGAVQL
                     IFAVAGFVLVVAAFADWVWNTYRNMGGSETEAGPFPWFGKTGAILFIGAWLLSWITSL
                     SLLREGRETEKRELQSNLPPKIKRD"
BASE COUNT         1408 a         1780 c         1777 g         1398 t
ORIGIN      
        1 ctggtgcttt tgggcgagaa attcttccag caacccgagt tcgtacatca actccaacgt
       61 cgagggatga atggtatcgc cgcgaaaatc gcggaaaaaa tccttgtgct tttccaacac
      121 gaggacgtcc acacccgcgc gagccaggag aaatccgagc atcacgccag caggcccgcc
      181 cccggcaatg caacaggtcg tgttttcagg caatgagctt tgctccattt tatctccttc
      241 cagaacttag cacacgttcg gttctattcg agcccgcccg gcgaatgcgg aagccgttgt
      301 cataggttgc cgtgcttcgc tccaaattgc ctgggttata cgatgtcgtg tctggcaggt
      361 aaatcggcgt gttctgctgg tgacagatta ccatgttgaa gtatgttggc ttcggtcatt
      421 gacatggctc cgctccaggc aatgacccat catttccgca ttgggcaact caaacaagga
      481 ttcaaaatgc aaaaacgcaa acttggcaaa agtaatctgg aagtctcggc catcggactc
      541 ggctgcatgg gaatgagttt ttcctacggc ccgcccaagg acaaacacga gatgatcaaa
      601 ctaattcacg cagccgtgga gcgtggaatc acattcttcg acacggctga agtttacggc
      661 cccttcacca acgaagaact tgtgggagaa gcgctcgagc cgtaccgcag ccaggtggtg
      721 atcgccacca agttcgggtt cgacctcagc ggcagcgata cgcggccagg tgcggcgggc
      781 ttgaacagca ggccggagca catcaagcag gctgtcgaag gctcgctcaa gcggctcagg
      841 gtcgggacga ttgatttgct ttatcagcat cgggttgacc cgaacgtgcc catcgaagac
      901 gtggcgggaa ccgtcaaggc gctgattcaa cagggcaaag tcaaacactt cggcctgtcc
      961 gaagccggcg tgcaaaccat ccggcgcgcg cacaaagtcc agccggtcac agccctccag
     1021 aacgaatact cgctttggtg gcgacgcccg gatgccgaag tgatcccgac acttgaagaa
     1081 ctcggcatcg gcttggtcgc ctacagtcct ttgggcaagg gttttctcac gggcaagatc
     1141 gatgaaaacg ccaggttcga cagttctgat tttcgcagca cgctgccgcg cttcacgccc
     1201 gatgcgctca agacaaatca ggcattgatt gatttgcttg agaaaatcgc ccaacgcaaa
     1261 aaagcaacac cggctcaaat cgcgctggca tggttgttgg cgcagaagcc gtggattgtt
     1321 cccattcccg gcacgacgaa gccgggtcgt ttggaagaaa acatcaaagc agttgaaatc
     1381 aaactcacac cggaagacct tcgcgaaatc gaaagcgcgg cgtcgaagat taaagtggaa
     1441 ggggcgcggt atccggaaaa gctggagcaa atgaccaacc ggtgacaatc ggcacgacag
     1501 gagggagcca aaatcatgag tgataacatc aaggaaaagg gatcaaatat ggaaatcaaa
     1561 agatgcggca cacagccttc caacaaagga ccggccgatt ggttcaccgg cacggttcgg
     1621 atcgaccctc tgtttcaagc aaacgctccg gcacgcgcgg taggcgccag tgtcacgttc
     1681 gagcctggcg cgcggacggc gtggcacacc catccgctgg gccaaaccct gatcgtgacc
     1741 gctggttgcg gccgcgcgca gcgatgggga ggtcccatcg aggaaattcg gccgggagac
     1801 gtgatttggt ttccgcccgg tgagaagcat tggcacggcg ctgcgtcaac cacggccatg
     1861 acccacaccg ccatccagga acagctcgac ggaaaaaccg tggactggtt ggaaaaagtc
     1921 accgacgaac aatatcaatc ctgattccac ggccgttcct cccacaaaaa ctgaggcaac
     1981 tcagaggatt tcagagtggg aaaacaaatt attttgtgcc atacgaagcg cgcagttcgc
     2041 ctgcgatttt ttctaatcgt ggcttgtccc gcgctatcag cgccatattc cagccattgg
     2101 ccgcaaacaa ttttgcgaac tcaagcccga tgtcctgaga cgcgccggtg atcaaagccc
     2161 atcgtttcat gcatccgatg gcagcacaaa agattggttg tgcgaatgcg agcgtaggct
     2221 caacacccca gcgcaatcgt ttagcttctg cgctaggttt ttttggcatc acccggagaa
     2281 gatcgccgga tggttccgga acttagaaag gtaaaactat gttaaggtgg gcagccattt
     2341 ttctaatcat cgcaatcata gcggcactat ttggatttgg gggcattgct ggcgcagccg
     2401 caggcatcgc caagaccctt ttcttcatct ttatcgcgat ttgcgtgatt ttgtttctgg
     2461 tgggtgtatt tgccggacga aacgtgtagc agcgcgaagt gttgcgccgg ttcgttggaa
     2521 gatttgacca gcgttaggtc aggagaagga ttttcttccc ctgttcacag ggcgttgagt
     2581 tacgcccaaa tcagccggcc actaattcca agacttcttc gcgcgtcaaa gtttcacgcg
     2641 cgagcaatgc ttgggcgact cgttcagcat cttccttttg ggcctcaatc atcgccagcg
     2701 cttcggcgtc cgcggcggca agaatgcgat tgatctcgat ttccgcttcg cgttgttgcg
     2761 gctcgtaata aagtttttcg cccatgcccc attcgaggac catcttgcgc gcaagttgac
     2821 gcgccatttc gaggtcgccg ctcgcgccgt tggttgtcgc gcccaaaaat gtcttttccg
     2881 ccgcgcgtcc gcccatcaac acggtcaatt gctgcagcaa catgtcccgg ctgtggatgt
     2941 tctggtcttc cttcggcaaa agcgttgtcg tgcccagcgc ttgaccgcgc gggatgatgc
     3001 tcaccttgta taacggcggc agcaaagttt ttttcaaatt gatgagcgcg tggcccgctt
     3061 cgtgataggc gatgagttcg cgttctttct gtgacaacac catggatttg cgttcctggc
     3121 cgaagcgcac tttgtcgcga gacgcttcca ggtccgcgag aaaaatttgc tcgcgatttt
     3181 gttgcgcgca caggatggcc gcctcgttga gaaggttcgc caaatcggcg ccggacatgc
     3241 ccggcgtggt ttgggcgatg atttccagcg cggattcttc agcgatcggc ttttgccatg
     3301 cgtgcgcctg caaaatcgcg cggcgtcctt tgacatcggg atatggcacc tgcactttgc
     3361 ggtcaaagcg ccccggccgc atcaatgctt catccaaatc ttccgggcgg ttcgtcgccg
     3421 cgacgaccac gatgccttcg ctgctttcga aaccatccat cgcggcgagc aatgcgttgt
     3481 tggtctgctg caattcgctg tttgtatcga gcttgcggtt tttgccgagg caatcaatct
     3541 cgtcaatgaa gatgatggcc ggtttgtgct tggcggcttg tttgaaaaga ctgcgcacac
     3601 gcttcgcgcc aacgccaacg aacacttcat tgaaatcact tccgtgcgcg ctgaaaaaat
     3661 tcgcctgcgc ttcgccggca atggccttgg caagcagcgt cttgcccgtc ccgggcgggc
     3721 cgatgagcag gacacctttt ggcaatttgc cgccgagctt gcggtacttg gccgggtccc
     3781 gcaggaaatc cacgacttcc tggatctccg ccttggcttc ctccacgccc gcgacgtcgc
     3841 tgaagcgaac agttggacgt tgccggatgc ggctgtgttg tccgcgcccg agccccgttt
     3901 ggaacgcgac cagtccgacg accaaaccga tcaacacgag cgcggaaaag atgttcaccc
     3961 attgttcttt aagcccgccg cccggcaatt cgatcaccac tctcggttgc gcagtcaggc
     4021 tcttgagttg ctccggatca agatgcgtgg tgatgctgaa cggctgctcc ttcccgcccg
     4081 attgccatgt gccttgaacg tgaaaaatac ccgcgtaagg tgtcggcacg atatggcctt
     4141 ggtggatgag tttgccgccc atgagcaatt ccaattgcgc gtggctgatg tcatgcgcga
     4201 caggacgcgt aaaataataa accgccgccg ccgatgcgag gccggcgatg gcaatggtga
     4261 gggcgatcag attccaaaag cgacgtgaac gttttttcat ggttgcaagg gaatgaggga
     4321 tgcgcaggtt tcgagcgtga tattagtggg aatgatttgc ggggtggttg aatccgcgta
     4381 cgcctcagcc caacgcgcgt tgagttgggc catctccacg cgggcgtgtg tccgatgcca
     4441 atattgcgca gcgcggtgcg caacgaccaa caatgtgatg cttaccaaag tgctgactgc
     4501 ctttgccatg catatacgat ggctgaacgg catttttcgg aaatcagaac cgggctgatt
     4561 atcttgtcag agtaacgctg acaaaggagg ttcaaggtcc aaggtttaaa gttcaaagtt
     4621 attacaggcc agcacgcctc cacattccaa ctttaaactt taaacttact aagctgcccc
     4681 atccacgatt tggcaaagga tgggctgcat cttatggccg gtgcgttcgc cgtattggcg
     4741 ggccatcgca cccatgaaat cttccgcctg atgatgcgcg acgaggttga tggtcgcgcc
     4801 gccaaaaccg ccgccggtaa ggcgtgcgcc gaggcagccg ggatgcgcgc gggccagttc
     4861 caccagcatg tccagttcag gcacgctgtt cttcaaaaaa tcgcgcgagc tttcgtggct
     4921 ctggaacatg tattgaccga attgccggtg gtcgtcctcg cgcaaagcgc ggctggcaaa
     4981 gatcactcgt tggatctcgc tcgtcacgtg atgcgcgcat tcaaattcgc gctccgtcaa
     5041 tttggccctg ttcgccgcga gcattttggg gtccacagaa cgcaatgttt cgaagccaag
     5101 cttgtgcgcg gcggattcgc acaggcggcg tagttcattg taggtgcctt caatgagcgc
     5161 atgtttcacg cccgaattgc agacgatgat ggcttcgccc gtgagcggca aaatttcgac
     5221 cgtgagcgaa cgaaaatcaa tttccatcac gtgccatgcc ttgccgaaga gcgaagaaat
     5281 ctggtcgagc aacccgacgc gcacgccgac aaaatcactt tccgcggcgc ggcagatttt
     5341 tgctgtttcc atcctttcaa cacgcgtcag ccccggcaaa tgtccccggc catcgcgcgc
     5401 tggcggcttt gcgatgccgg tctcgccgag cgaatacggg aaaagctggc ggacggtcag
     5461 cgcggtggcg acttcgagcg cggcggaact gctcatgccc gcgcccatcg ggatggtgcc
     5521 gtcaatcgcg gcattgaaac cgctgatatt tacgccccgt ttgcgaagct ggtcgagcac
     5581 gcccttcacg taatcggccc agtgcgcctc gggatttttt ttgatctggt cggcccaaaa
     5641 aatttccggc ttcggaaaag cggtggagat gagttcgatt ttgccgtccg tgcgcggcgc
     5701 ggcggcgatg tggacatatt tgtccactgc aagcgaaagc acgattccct cgttgtaatc
     5761 ggtgtgattg ccgagcagtt ccaaccggcc gggagcgcgg atgagatgga ccgcgggcga
     5821 gccaaaatgt tttttgaaaa gtgattgcgc gtcgtttgac atgaaattag tgctcaataa
     5881 aaaagccaga cagccaaatt aatcaatcca aagttgcatg aggaacttta aaatgccagc
     5941 gcaagacgat cattttccgg ttcagtcacg tttaattttg ggcggcaggt ttgattgcag
     6001 ttcccttttc tcggtttccc gcccttcgcg cagcaggctg aggctcgtga tccacgaaag
     6061 gagccacgcg ccgatgaaca ggattgcgcc cgttttccca aaccatggaa aaggccccgc
     6121 ctcggtttcc gaaccaccca tgttgcggta cgtgttccaa acccaatcgg cgaaagccgc
     6181 caccacgaga acgaaaccgg caaccgcaaa tattaattgc accgcgcccg cgagaaaacg
     6241 ccggcccatg atcgaaccca atcccggcgt ggcaaactgg ttgaccagca ggcaattcat
     6301 cgccgcgctg cgtttaacgg ttttgccggc cgaaaatatc ttcatacgcg tacatcaggg
     6361 cga
//
LOCUS       DASZMM010000323         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4804856, whole genome shotgun sequence.
ACCESSION   DASZMM010000323 DASZMM010000000
VERSION     DASZMM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4804856"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VG754_07800"
     CDS             <1..139
                     /locus_tag="VG754_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HWF19154.1"
                     /translation="NGERLELTHKASSRETFANGALRAAQWVMKQKPGLYDMQDVLGL
                     K"
     gene            165..653
                     /gene="folK"
                     /locus_tag="VG754_07805"
     CDS             165..653
                     /gene="folK"
                     /locus_tag="VG754_07805"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWF19155.1"
                     /translation="MIYIALGSNLGDSREIIQRAMERLREFSTGPLLKSSLWQTTPVD
                     CPPGSPPFVNAVIGLQPLPGETPESLLKKLQSLEKEFGRRPKKVMNEPRPLDLDLIAF
                     GIATRNTQQLVLPHPRAHERRFVLQPLSEIAPDVVIPGQSKTVVQLLAALPGDAAMSK
                     LV"
     gene            complement(650..1987)
                     /locus_tag="VG754_07810"
     CDS             complement(650..1987)
                     /locus_tag="VG754_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF19156.1"
                     /translation="MNSAIRLKLFLMMVLEFFIWGAWLPLIFGYLPSLGFRPLQQALI
                     LNAFPAAAIVGMFFSNQFADRNFAAEKFLAFSHLIGGIAMIGLAFTKAFWPFFGLMLV
                     HCLFYVPTMSITNSIAFANMKDAKDFGIVRMGGTLGWILAAWPFTFILVDWNAVRAAN
                     PQGIADWIGGALAHGLSGPALMAATVWTFIVAGVASLVLAGFSLMLPHTPPKRAGEGG
                     AEKLAWVEALKLLKHPFVLVLWLVTFVDAFVHNSYFAWAGSFLGAPANAGGAGIPGNW
                     IMPVMSVGQIAEMLTMFILGATLKRLGWRMTMILGIIAYPIRFAVWAFLPEHRELIIL
                     VQVLHGVCYAFFFATVYIFAEEYFPKDVRSSAQGLFNFMILGLGALVAYTVCPWLSQT
                     VFVQNGTTNWHQLFLVPLFVALGSAATLALFFHPPKTMVESGASTRKDETVRA"
     gene            2050..2703
                     /locus_tag="VG754_07815"
     CDS             2050..2703
                     /locus_tag="VG754_07815"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HWF19157.1"
                     /translation="MTAVRALDFCQGNVYFISMRAAFKEWAVVVDALGRGEQILILRK
                     GGISEGRGGFKMEHPRFFLFPTLFHQQRESVIPSAQARFDEIAPQFPPPEKVRLEFFA
                     EVIFAERLLSLSDANALRGQHVWRDEVIAERFEWGREKAIFALGVKVFRLPQPIELPM
                     SSSYGGCKSWIELEREIAAEGSRPVLDEQVFQEKLARLRMALSRSAQFSDTPSLHSP"
     gene            2700..>3132
                     /locus_tag="VG754_07820"
     CDS             2700..>3132
                     /locus_tag="VG754_07820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWF19158.1"
                     /translation="MTAPPFILASSSPRRSELLRSLGLDFSVIPSDANEIHNEQLTAI
                     EISQLNAYRKARAVAKQFPDALILGADTLVYLGTTLFGKPRNMEDAYRMLKQLQGKTH
                     EVVTGVCLLRLRGHRQTIFAESTRVTFRPLGVKEIRDYMGRI"
BASE COUNT          759 a          873 c          862 g          638 t
ORIGIN      
        1 gaacggcgag cggttggaat tgacgcacaa agcttcgagc cgcgagactt ttgccaacgg
       61 cgcgttgcgc gcggcgcaat gggttatgaa gcaaaaaccc ggcctttacg acatgcagga
      121 tgtgctggga ttgaaataga ttccgttctc caggagacgc aactttgatc tacatcgccc
      181 tcggctcgaa tctcggtgat tcgcgcgaaa tcatccaacg cgcgatggaa cggttgcgcg
      241 aattttcgac cgggccgttg ctgaaatctt cactctggca aacgacaccc gtcgattgtc
      301 cgcccggttc gccgccgttt gtcaacgccg tcatcggctt gcaaccgctt cccggtgaaa
      361 ctcccgaatc cctgctcaaa aaattgcaat cactggaaaa agaatttggc cgccggccaa
      421 aaaaagtcat gaacgaaccg cgcccgcttg atttggattt gatcgctttt ggcattgcaa
      481 cgcgcaatac gcagcaattg gttcttccgc atccacgcgc gcatgaacgt cgtttcgtgc
      541 ttcaacccct cagtgaaatc gcgccggatg tggtaatccc gggacaatcc aaaacagtcg
      601 tgcaattgct ggctgctttg ccgggtgatg cagcgatgag taaacttgtt taggcccgca
      661 ccgtttcgtc ttttcgggtg gaagcgccgg attccaccat cgtcttgggc ggatggaaaa
      721 aaagcgccag ggttgccgcg gagccgaggg ccacaaaaag cggcacgaga aatagctgat
      781 gccaattggt cgtgccgttt tgcacaaaaa cagtttggga gagccacgga caaacggtat
      841 aggccaccag cgcgccaaga ccgagaatca tgaaattaaa caaaccttgg gcgcttgaac
      901 gcacgtcttt tggaaaatat tcctcggcaa aaatataaac tgtcgcaaag aagaaggcat
      961 agcagacccc gtgcaaaact tgcactagga tgatcaactc cctgtgttcc ggcaaaaagg
     1021 cccacaccgc aaaacgaatc gggtaggcga tgatcccgag gatcattgtc atgcgccagc
     1081 cgagccgttt gagcgttgcg ccgagaatga acatcgtgag catttcggca atttggccaa
     1141 cgctcatcac cggcatgatc cagttaccgg gaatgcctgc gccgccggcg ttggccggcg
     1201 ccccgagaaa cgaacctgcc cacgcaaaat aggaattgtg aacgaaagcg tccacgaagg
     1261 tcaccagcca tagcacgagc acaaatggat gtttgagcag cttcaacgcc tcaacccacg
     1321 cgagcttttc cgcgccacct tcaccggccc tcttgggcgg cgtatgtggc agcatcaagc
     1381 tgaagcccgc gagaacaagc gacgcgacgc ctgcgacgat aaatgtccaa acggtcgccg
     1441 ccatcaaagc ggggccgctt aatccgtgcg cgagcgcgcc tcctatccag tccgcgatgc
     1501 cttgtggatt ggcggcgcgc acagcgttcc aatcaaccaa aatgaacgtg aatggccacg
     1561 cggcgaggat ccagccgagc gttccgccca tgcggacaat tccgaaatcc ttcgcgtcct
     1621 tcatgttcgc gaacgcgatg gaattcgtta tggacatagt gggaacataa aaaagacaat
     1681 ggaccagcat cagcccaaag aacggccaga aagcctttgt gaatgccagg ccaatcattg
     1741 cgattccgcc aatcaggtgg ctgaaggcca gaaatttttc cgcggcaaaa ttacgatcgg
     1801 caaattgatt gctgaaaaac atgcccacga ttgccgcagc cggaaacgcg ttcaggatga
     1861 gcgcctgctg caacgggcgg aatccaaggc tcggcaaata gccgaaaatc aaagggagcc
     1921 acgcgcccca aatgaaaaat tccaaaacca tcatgaggaa caatttgaga cgaatggcgc
     1981 tattcataat tgatgaccgc agcttaggtc attgcggcaa acatggcaag atgaagctag
     2041 aaaatggccg tgaccgctgt gcgtgcgctc gacttttgcc agggcaacgt ctattttatt
     2101 tccatgcgtg cggcgttcaa agaatgggcg gtcgttgtgg atgcgctcgg gcgcggggaa
     2161 caaattctca tcctgcgcaa gggcggcatc agcgagggcc gtggcgggtt caagatggaa
     2221 catccccgtt tttttctatt tccgacactc tttcatcagc agcgagaatc ggtcattcca
     2281 tcggcgcagg cgcgctttga tgagatagca ccacaatttc caccgccaga gaaagtccgc
     2341 ctcgaattct ttgccgaagt catttttgct gagcgattgc tttctttgtc tgacgcgaac
     2401 gccttgcgcg ggcaacatgt ctggcgcgat gaggtgattg cagaacgctt tgaatggggc
     2461 cgcgagaagg cgattttcgc gctcggggtc aaagttttcc ggctgcccca accgatcgaa
     2521 ttgccgatgt cgtcctcgta cggcggatgc aaatcgtgga tcgaactgga gcgcgaaatt
     2581 gcggcggaag ggagcaggcc ggttctcgac gagcaggttt ttcaggaaaa actcgcgcgc
     2641 cttcgcatgg cattaagccg cagcgcccaa ttctccgaca ctccgtcgct gcactcaccg
     2701 tgaccgctcc gccattcatc ctggcttcga gttcgccccg gcgctccgaa ttgctccgca
     2761 gcttgggctt ggattttagc gtcattccga gcgacgcaaa cgaaatccac aacgaacaat
     2821 tgaccgcgat tgaaatttcc cagctcaacg cctatcgcaa ggcgcgcgcg gtggcgaagc
     2881 aatttcccga cgccttgatc ttgggcgcgg atacgctggt gtatctcggc acgactttgt
     2941 tcggcaaacc gcgcaatatg gaggacgcct accggatgct gaaacaattg cagggcaaga
     3001 cgcatgaagt cgtgaccggc gtttgcctgc tgcgattgcg cgggcatcgg cagacaattt
     3061 ttgccgagag cacgcgcgtc acgtttcgtc cgcttggcgt gaaggaaatc cgcgattaca
     3121 tgggccgaat ca
//
LOCUS       DASZMM010000324        15575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5354642, whole genome shotgun sequence.
ACCESSION   DASZMM010000324 DASZMM010000000
VERSION     DASZMM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 15575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15575
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5354642"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..2361
                     /locus_tag="VG754_07825"
     CDS             238..2361
                     /locus_tag="VG754_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HWF19159.1"
                     /translation="MKHQRGWVFAIAFLLTGLRASGQANLPIYTDNLVNGFQNWSWAA
                     NNLSNTSPVHSGSQSISVSSEAWQALSFYHPDFDTTPYTNLTFWINGGAGGQIIQVVG
                     ELSQVSQAAYVLPALAPNIWQQITIPLSTLGVADKTNFDRFSLQLTASGASNTFYVDE
                     MQLTAKPAPALVHINVSVNQILRTVDARFFGVNTAVWDSELNTPTTVSLLREAGLTTL
                     RFPGGSLSDEYHWGSNTSGTNTWTWGTSFTDFARVATNIGAQVFITVNYGTGTPAEAA
                     AWVKNANITNHYGFKYWEIGNELYGTWETDSNTYPHDAYTYATRAQQYIQQMKAADPT
                     IKIGVVVTPGETSDSNGYTNHPAYNPRTGQTNYGWTAVLFSTLKSLGVTPDFAIHHRY
                     PEYTDSESDPLLLQGTSGWASDAADLRQQITDYFGAGGTNIELLCTENNSNSGNQGKQ
                     SVSLVNGLYYADSLAQLMKTEFNSLCWWDLRNGLDNSGNLDPTLYGWRMYGDIGMLNG
                     LGDVLTNRYPHFFTAKLMQYFARAGDTVLGAASDYELLSAYGARHADGSLSLLVINKD
                     SIGTFTGQIVLGGFVPSSSATTHSYGMPQDNAAQTGIGSCDIAQTNFSGAGTNFNYVF
                     KPYSATVLTFAPTAASLTLLPQPSPGKIAFQLHGQPGVRYVIQSSTNLAVWSSASTNT
                     LAASSLNVTNARTPSDEFWRVVWVP"
     gene            complement(2407..4242)
                     /locus_tag="VG754_07830"
     CDS             complement(2407..4242)
                     /locus_tag="VG754_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF19160.1"
                     /translation="MRFSGHSLKKLLPCLILLGVLTALSCLASGPPAVITNQPLDTNV
                     PLGGTAGFQVYASSGTTMTYQWLKNGTSISGATQSSYTISNVKLSDQGSYSVQVINGG
                     GTVTSSSATLTVINVAPKITTQPQDGAYYTNNPVTLSVVASGTAPLLYQWALNGTNVP
                     GATGSSLYYAHITTNQAGNYTVIVSNVVGVVTSSVAVVTVQAAPYITSQPQSQDGLLG
                     LTVTFTVSAMSAGPMTYQWYYQGSPVGGATSSTYSVLNLLGSDAGDYFVIISNAQGST
                     MSSIAHLGVVYTPTIKRQPVSQTVALGQSATFTVSGTNYPINYQWYFKGSALSGATDS
                     SYAVNNAQTNNAGNYWVTLSNSSGSVTSSTATLTVNLPASISTPPQNQTLVVGQNAIF
                     TVALSGTSPFSYQWSYNGNALSGATTSTLSVTNVQTNNAGSYSVAVGNSWGPATNATA
                     TLTVLVPPSITTPPNSQTAVVGQSVTFSVVATGTRPLKYQWYYNGSKIEEAGDSSLTL
                     NSIQGSSTGNYKVVVTNNAGSVTSTVAILTVANPPTLQTTGMAGNGFAVQFSVPSGHT
                     YIVMASTNLSNWAPIYTNSAQTTNITFSDSNSVSYPCRFYRVMVQ"
     gene            complement(4293..6404)
                     /locus_tag="VG754_07835"
     CDS             complement(4293..6404)
                     /locus_tag="VG754_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF19161.1"
                     /translation="MRLENENRPYSSPAELSGGKCYSPGLRVPRGLVHFFIKSIFFAL
                     ILFLLTDAHCADSTNNINSTNIADLSLEDLMKIEVPVVTSASKVAQKTTEAPASVTVI
                     NADEIQRYGQRTLADVLRSVQGFNVSYDRNYAFLDARGLSLGDFNSRVLLLVDGHRVN
                     NNLTDGAFIDTAFILDVDLIDHVEIIRGPNSVLYGNNAFFGVINVVTRQGRQINGLET
                     SAEYGSFDEYKVRATYGKLFTNGLEMLVSGTYYDNPGPPSLFYKEFDTPTQNNGVANA
                     NGLDEDKYGSLFGSLSYEDFSLESAYISREKGNPTAQYSLTTFNDPRLRTTDDRSYVA
                     LKFAHSFPDVVDVTARVYYDRYRHEIGYPQSLVVGTNVLFSGFTLENDTGQWWGSELE
                     LNKQVWEKHTLTVGAEYRDDFHQEQDITGQPSVMLTRQSYGVYGQGDFELLANLHLEA
                     GVRYDKSTDFPSAVTPRLALIYHPFTNSTIKAIYGTAFRTPNFSELSDPRFRDINPER
                     ISTYELIYEQQIGPHVRSSLSGFYNKMDNLIVFNSGSFTNINAETEGVELALEGVFAD
                     GIRGRASYSYQHTRDETLSWSMPDSPSHMVKLNLSVPVWQEKIFADLEFQYVSDRASL
                     HNTTDSGGEPLTVQGQTAGAYEIVNFTIFSRELVKNLEVSASIYNLFNAHYSDPASRF
                     HTQDLIEQDGRTFRVKATYRF"
     gene            6539..7105
                     /locus_tag="VG754_07840"
     CDS             6539..7105
                     /locus_tag="VG754_07840"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HWF19162.1"
                     /translation="MQCQHPLFHAFVLICLALTGWHMPGAARAQGNQISEYQVKAAFL
                     FNFVKFVEWPSDSFPTAETPIIIGVLGTNPFGGDLDAVIHNKTVNNRPIEIRQLHLMS
                     ECTNCHVLFISGMVKKRVGEVLSTVGNASVLTVGESDGFTEAGGIINFVREGNKFRFQ
                     INAEAAKKAKLKISSKLLGLALPSNSNH"
     gene            7349..9337
                     /locus_tag="VG754_07845"
     CDS             7349..9337
                     /locus_tag="VG754_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWF19163.1"
                     /translation="MKLFRDLPIQQKTLVMTLSICGAVLCIAIAVLFTFQVLNFRANY
                     QRDLDTVAVIIANNGTAALAFKDDKAATELVDSLRAKPSILTASLLLPDGSMFVHYGR
                     PENAAKLAQFPPAGQQLFVGGDLLLTQPVELKGERLGTLYIRADYHRTFLELLHVYGL
                     VILGIVAASIGLAAFLSAKLGKSITQPVLILAQAAQIVGEKKDYSVRVPASSQGDELG
                     QLTRAFNEMLSRIQSQDAALSLSQQKMEALIHSIDGIVWERTADAHKFTFVSRQSEEI
                     LGYAPALWCEKEDFWESKLHPQDAAKARQTNREMAARGQPYSYEYRMLAADDRTVWIR
                     ESGTVLMENGKPIAMRGIFQDITQSKLDAQNLDKLNRQLMDTSRQAGMAEVATGVLHN
                     VGNILNSVSVSATLAGDKLRQSKIGNLREATALLNEQNGHLVEFLTNDPGGKVLPTYL
                     SAMADQIAGEQANLLTEISSINQNVEHIKEIVAMQQSYAKVSGVYENLSVAGLVEDAL
                     RMNIAAFDRHSIEVIKEFDENMPHASVDRHKILQILINLLRNAKHAMDHRPASERQMR
                     IRLCMASPDRVKISIKDNGIGIAPENLTKIFNHGFTTKKDGHGFGLHSGANAAKEMGG
                     SLSAHSHGLGHGAEFTLELPTAVPGRKEGEQTTTAVSL"
     gene            9334..11403
                     /locus_tag="VG754_07850"
     CDS             9334..11403
                     /locus_tag="VG754_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19164.1"
                     /translation="MNSKPLEPNRRILIIDDNASIHADFKKILCPVDSNNSEVNEMEA
                     LIFKRAKKNISQHVFHLDSAFQGQEALEMVKQSLKDGRPYATAFVDVRMPPGWDGIET
                     IARIWEVDPQIQIVICTAYADYSWEEMRGKVGQPDSLLVLKKPFDNIEVQQMAHALTK
                     KWLLNFQTVQQMADLTQANKALAELEERFSKAFHESPVPSGIQSLPDKRFVDVNHRLA
                     QIAGLRREDMIGRSPAELFLWEKPAAAEQWYESATGRQLVRAQEGKLREKGGRLHEVL
                     VSLCPLALGEKSHLLLLAEDVSEQALLERQLRQSQKMEAIGQLAAGVAHDFNNILTVI
                     QGHTGMIQLQMEPNSPLRKSLEQISLSTERASALIRQLLMFSRKQVMQFRHVDLNNTL
                     QNVLKMLERLLGETVQVEFHPQPFMPAIYADTSMLEQIAMNLAVNARDAMPNGGFVKI
                     LTSMQTIRRGPNALDAQARDGEFICLTFSDNGTGMDTQLINRIFEPFFTTKPAGKGTG
                     LGLSTVLGIVQQHSGWVEVESKPGKGTTFRLYFPKSERPAEKLEKIEETELLKGRETV
                     LVAEDEGALREMVALVLRTQGYTVLEAASGREAIEKWGRSERKVDLLVTDMVMPGGVL
                     GGELAECLQEKCPSLKVIYTSGYSPGMAGKDTSLLEGRNFLPKPYSIGKLAKFVRECL
                     DSPPVPC"
     gene            12151..13503
                     /locus_tag="VG754_07855"
     CDS             12151..13503
                     /locus_tag="VG754_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF19165.1"
                     /translation="MDFLIVDDDKTFRDATRLLIEDEGHYAQPAANGEAALAMLKEDK
                     FDAVLLDVNLGPENGLDVLNQLIKVQPNLPVVMFTAQGTIKTAVEAMRIGALDFLEKP
                     FTREQMHTVIARLKRFSVMNENIQRLEEEVNETKLQNPELLLDFASPGMRQIMEILQR
                     AARTPASILITGESGTGKSVLARAVHEQSHLADKPFVTVSCPSLSKELLESELFGHVK
                     GAFTGAVKDHWGKVKAAGGGTLFLDEIGDLPMEIQPKLLRLLQEREYERLGENITRQA
                     ELRIIAATNRDLKKRMTEGAFREDLYFRLNVIAVEMPPLRTRHDDMLRFAEHYLKYFS
                     KQCARPMPKISGPAATHMLAYTWPGNLRELRNAIERAVILAKGDELCAEDLPGDLDGD
                     SNHRDADLQPGALISLERLEELHMRKVLERTSTVTEAAHILGIDQATIYRKRKKLGMS
                     "
     gene            complement(13773..14174)
                     /locus_tag="VG754_07860"
     CDS             complement(13773..14174)
                     /locus_tag="VG754_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19166.1"
                     /translation="MRLKLKEDPKEWRKAAWLGALGFAGMTTVLRWRHILPTRGWMAA
                     LIGLALLSVFAWLRPGWFRGYYRFTSRVGFGITQLLGRIVLAALFVLVVTPFGLVLRL
                     LGKDLLQLKPQRDAKTFWHPAKKSSPLDRMF"
     gene            complement(14177..>15575)
                     /locus_tag="VG754_07865"
     CDS             complement(14177..>15575)
                     /locus_tag="VG754_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HWF19167.1"
                     /translation="AEAAILTIDGVGEWATTTIGVGRGQEIQLLEELRFPHSLGLLYS
                     AFTSYCGFRINSGEYKLMGLAPYGEPKYADAIRQNLIESKPDGSFRLNLDFFDFLGGT
                     TMTNGRFHRLFGGGPRGPEEPIERRHMDVARSIQVVTEEIMLGLAHRAREVTGLKDLC
                     MAGGVALNCVANGRILREKIFDRLWIQPAAGDAGGALGAALTAWHLDKTSQPDETRRP
                     AKTDAMRGGFLGPEYSDAEIEAVLQSNKAVYQRLSDDALLDMTVGLLRAGKVVGWFQG
                     RMEFGPRALGNRSILGDARSPQMQSVMNLKVKFRESFRPFAPVVRRERVADYFELDQE
                     SPYMLLVAPIKAELRRPVPPGVTGLELLKAEHSTLPAITHVDYSARIQTVAQTDNPLL
                     YDLLLRFEKATGCGVLVNTSFNVRGEPVVCTPDDAYRCFVNTEMDYLVIGSFVIERTA
                     QPKQTAERRVAPQAD"
BASE COUNT         3724 a         4268 c         4150 g         3433 t
ORIGIN      
        1 ctgaattggg gaaacgtcgg gcggctctgg aacattcgat gttcagcgtt cagcgttggt
       61 tgttcgatgt ttccggactc ttatgtggtc gaaaatccaa caaaaaatta accctaataa
      121 aggctaataa aggctaatta acgctaagat gaaaaaaata ttttcccgaa acgagcacat
      181 caccatgctg caaagcacag agcacactga attgatttgt ttgatatgtt tattctcatg
      241 aagcaccagc gcggatgggt cttcgcgata gcgtttctgc tgaccggctt gcgcgccagt
      301 ggacaggcca atctgcccat ttacacggac aatctcgtca atggctttca gaattggagt
      361 tgggccgcaa acaatctcag caacacttct ccagtccatt ccggcagcca atccatcagt
      421 gtgagttcgg aggcatggca ggcgctttcg ttttatcatc cggattttga cacaaccccg
      481 tacacaaacc tgactttctg gatcaatggc ggcgcaggcg gacagatcat ccaggttgtt
      541 ggcgaattaa gccaggtttc ccaagcagct tatgtgctgc ctgctttggc gcccaacatc
      601 tggcaacaga tcaccatccc gctctccaca ttgggcgtcg cagacaagac caatttcgac
      661 cggttttcgt tgcaactcac cgcctccggc gccagcaaca ccttttatgt ggatgagatg
      721 caactcaccg ccaaacccgc gcccgcgctg gtccacatca atgtcagcgt gaatcaaata
      781 ttgcgcacgg ttgatgcgcg gtttttcggc gtcaacaccg cggtttggga ttcagaactc
      841 aatacgccca cgacggtctc gttgctccgc gaagcaggtc tgacgacttt acgttttccc
      901 ggcggctcac tttccgacga gtatcattgg ggatcaaaca ccagtggcac aaacacgtgg
      961 acttggggga cttcattcac cgacttcgcc cgggtggcga caaatatcgg cgcgcaggtt
     1021 tttatcactg tgaattacgg caccggcact cctgccgaag ccgcggcatg ggtcaaaaat
     1081 gccaacatca cgaatcatta cggttttaaa tattgggaaa tcggcaatga actttacgga
     1141 acatgggaaa ccgactcgaa cacgtacccg catgatgcct acacttatgc cacgcgggcg
     1201 cagcaatata ttcagcagat gaaagccgcc gatcccacga tcaaaatcgg cgtggtggtt
     1261 actcccggtg aaacgagtga ttccaacggt tacaccaatc atccagcgta taatccgcgc
     1321 actggccaga ccaattacgg ttggacagcg gtgttgttca gcacattgaa aagtttgggt
     1381 gtcacgccgg attttgccat ccaccatcgc tatcccgaat atacggacag cgaaagcgat
     1441 ccgctcctgc ttcaaggaac cagcggctgg gcttcagacg ccgctgattt acggcagcaa
     1501 atcactgatt actttggcgc gggcgggaca aacatcgagt tgctctgcac ggaaaacaac
     1561 agcaattccg ggaaccaggg caaacaatca gtcagtctcg tcaacggcct ttattacgcc
     1621 gacagcctcg cgcaactgat gaagaccgag ttcaattctc tctgttggtg ggaccttcgc
     1681 aatggcttgg ataacagcgg caacctggac ccgacgctat acggctggcg gatgtacggc
     1741 gacatcggga tgctcaacgg ccttggcgat gttttgacca accgttatcc acatttcttc
     1801 accgcgaagc tcatgcaata cttcgcccgt gccggtgata ccgtgctggg cgccgcaagt
     1861 gattacgaat tgctttcagc gtacggcgcg cgccatgcgg acggttcgtt gtccctgctc
     1921 gtcatcaaca aagattcgat tggaactttt accggccaaa tcgttttggg cggttttgtc
     1981 ccatcctcat ccgccacgac tcattcctat ggaatgccgc aggacaatgc cgcgcagacc
     2041 ggcatcggct cgtgtgacat cgctcaaacg aatttttcag gcgcgggcac gaatttcaat
     2101 tatgttttca aaccctattc cgccacggtc ctcacattcg ctccaaccgc ggcatcattg
     2161 actcttctgc cgcaaccttc accgggaaaa attgcattcc aactccacgg ccaaccgggt
     2221 gttcgttacg tcatccaaag ctcgacaaat ttggccgttt ggagttcagc gtccacgaat
     2281 acattggccg cttcttcgct gaatgtgacc aatgcgcgca cgccttccga cgaattttgg
     2341 agggtcgtat gggtgccctg attatggagc gtcggccttg aacgcaatca tacctgatcg
     2401 aagggactat tgaaccatca cacgataaaa gcggcaggga tagctcacgg aattggaatc
     2461 ggagaaggtg atgttggtgg tttgcgcgct gttggtgtag atgggggccc aattagaaag
     2521 attcgtggac gccatgacaa tgtaagtgtg tccgctcggc acggagaatt ggaccgcaaa
     2581 tccgttccct gccatgccgg tggtttggag agttggagga ttggcaacgg tgagtattgc
     2641 cacagtgctg gtcaccgagc cagcgttgtt ggtcaccaca accttgtaat tgccggtgga
     2701 actgccctgg atcgagttca aggtaagtga tgaatcaccc gcttcctcga tctttgatcc
     2761 attatagtac cactgatact taagcggtct tgtgcccgtg gccacgacac tgaaagtaac
     2821 gctctggcca acgaccgcgg tctgggagtt cggcggtgtc gtgatggaag gcggcacgag
     2881 gacggtcaat gttgctgtag cgttggtcgc cggaccccag gaattcccaa cggccacact
     2941 gtagctgccg gcgttgtttg tctggacatt cgtcactgaa agagtggaag tcgtcgcgcc
     3001 cgacaatgca tttccattat acgaccattg ataactaaat ggcgaagtcc cgcttaacgc
     3061 cacggtgaaa attgcgttct ggccaacgac aagcgtttgg ttttgcggtg gtgtcgagat
     3121 ggaagcggga agattgaccg tcagggtcgc agtcgaactg gtgacggagc cggaagagtt
     3181 gctcagcgtc acccaataat ttccagcatt gtttgtttga gcattattga ccgcgtatga
     3241 ggaatcggtc gctcctgaca aagccgagcc cttgaaatac cactgataat tgatgggata
     3301 attggttccc gacacggtga atgtcgcgct ctggccaagc gcgacggttt gcgaaaccgg
     3361 ctgccgcttg atggtgggag tgtacacaac ccccaaatgc gcaatgctgc tcatcgtgga
     3421 cccttgagca ttgctgatga tgacaaaata atcgcccgcg tcgcttccca gcagattgag
     3481 cacagaataa gtcgaggagg ttgctccacc gactggactg ccctggtaat accattgata
     3541 agtcatggga cccgcgctca tggccgacac ggtaaaggtg acggttaaac ccaacaaccc
     3601 atcctggctt tgcggctgcg aagtgatata aggcgccgct tgcaccgtga cgacagcgac
     3661 agaacttgtc accacgccca cgacgttcga aacgatgacg gtgtaattac ccgcctgatt
     3721 cgtggtgatg tgggcgtaat aaagagatga acccgtggcg ccgggcacat ttgttccgtt
     3781 caaagcccat tgatataaaa ggggagcggt gccggaggca accacagaca aggtgaccgg
     3841 gttgttagta tagtatgccc catcctgcgg ttgtgtggtg attttgggag cgacatttat
     3901 cacggtcaag gtcgcactgg aactggtgac tgttccgccg ccgttgatca cttgcacgga
     3961 gtagcttccc tggtcggaaa gtttcacatt tgagattgtg taggagcttt gagtggcacc
     4021 gctaatgctt gtaccatttt tcagccactg ataggtcatg gtggttccgc tgctcgcgta
     4081 cacttgaaac cccgcagtgc ctcctaatgg gacattagtg tccaaaggtt ggttggtaat
     4141 gactgcagga gggccactcg ccaggcaaga cagggcggtg agcacaccca atagaatcag
     4201 gcatggcagc aattttttta aactatgacc tgaaaatctc attttcgtcg caacgcgaca
     4261 ccagccaaaa agctattctc atgccatagt gtctaaaaac gatacgttgc cttgacacga
     4321 aatgtacggc cgtcctgctc aatcaagtcc tgcgtatgaa aacgcgaggc ggggtccgag
     4381 taatgcgcgt taaacaggtt gtagatgctt gcggagactt ccaggttttt gacgagttct
     4441 cggctgaaaa tggtgaaatt cacgatctca taagctcccg cagtctggcc ctggacggtc
     4501 aaaggttccc cgcccgaatc agtcgtgttg tgtaatgacg cgcggtcgct gacgtattgg
     4561 aattccaagt cggcaaagat tttttcctgc cagaccggca cactgaggtt aagcttgacc
     4621 atgtggctcg gcgaatcggg catggaccag gaaagggttt cgtcgcgcgt gtgctgatag
     4681 gagtaactgg cgcgcccgcg aatgccgtcg gcgaaaactc cttccaacgc cagttccacg
     4741 ccttccgttt ccgcatttat attcgtgaag ctgccgctgt tgaagacgat caagttgtcc
     4801 atcttgttgt aaaaaccgga aagtgatgaa cggacgtgcg gtccgatctg ctgttcgtaa
     4861 atcagctcgt acgtgctgat cctctccgga tttatgtcgc gaaaacgcgg gtcgctgagt
     4921 tcggaaaaat tgggcgtgcg aaaagcggtt ccatagatgg ccttgatggt ggagttggtg
     4981 aagggatgat agatcaacgc cagacgcggc gtgacagcag aggggaaatc agtggatttg
     5041 tcataccgca ctcccgcctc cagatgcaga ttggcgagca attcgaaatc accctggccg
     5101 taaacgccgt aactttgcct cgtcagcatt acggacggct gaccggtgat gtcctgttcc
     5161 tgatggaagt catcgcgata ttccgcgccc accgtcaagg tgtgcttctc ccaaacctgt
     5221 ttgttcaatt ccaattccga gccccaccat tggccggtgt cattttccag ggtgaacccg
     5281 ctgaacaaca cgttggttcc caccaccaac gattgcggat agccgatttc gtgacggtaa
     5341 cggtcgtaat aaacccgcgc agtcacatcc accacatcag gaaagctgtg cgcgaattta
     5401 agagcgacgt aactgcggtc atcggtggtt cgcaaccggg gatcgttgaa tgtcgtgagg
     5461 gaatattggg ccgtgggatt acctttttcg cgggaaatgt aggcgctttc caagctgaaa
     5521 tcttcgtagc tcagggaacc aaaaaggctt ccgtatttgt cttcatccaa cccgttcgcg
     5581 tttgccacgc cgttgttttg cgtgggagtg tcaaattcct tgtagaacag gcttggaggc
     5641 cccggattgt cgtaataggt tcccgacacg agcatttcca acccgtttgt gaaaagtttt
     5701 ccatacgttg cccgcacttt gtattcatca aaagaaccat attcggccga agtttccaga
     5761 ccgttgattt gcctgccctg acgcgttact acattgatca cgccgaaaaa cgcgttgttg
     5821 ccgtagagca ctgaatttgg gccgcggatg atttcgacgt gatcaatcaa atcaacatca
     5881 aggatgaatg ctgtgtcgat gaacgcgccg tcagtcaggt tgttgttgac gcgatgaccg
     5941 tccaccagga gcaatacgcg gctgttgaag tcaccgagac tcaacccgcg cgcatcgaga
     6001 aatgcataat tgcgatcata cgaaacattg aagccttgca ccgagcgcaa cacatcagcc
     6061 agtgtgcgct gcccgtatct ctgaatttca tccgcgttga tgaccgtgac ggaagcaggc
     6121 gcttcagtag ttttttgggc gacttttgag gcgctggtca ccaccggcac ctcaatcttc
     6181 atcaagtctt cgagtgaaag atcggcaatg ttagtcgagt tgatgttgtt ggtggagtcg
     6241 gcgcagtgag cgtccgtgag caaaaagagg ataagcgcga aaaaaatgga cttgataaaa
     6301 aaatgaacca agccgcgcgg cacacgaagg ccgggagaat agcatttccc cccgctcaac
     6361 tccgccgggc tgctgtatgg ccgattttca ttttcaagtc tcattttgcg cgcggcatca
     6421 acagtgattc agccgtgccg gagcagtttc tgttgcgaca gcaaatcaaa aatgttacca
     6481 ctgcgacata aacttgcggg atagacaaag cacgtccggt gccaatgggg acaggggcat
     6541 gcaatgccaa catccgctgt tccatgcgtt tgtgttgatt tgccttgctt taacaggatg
     6601 gcacatgccc ggagcggcgc gtgcccaagg caaccaaatt tcggaatatc aggtgaaagc
     6661 cgcattcctg ttcaattttg tcaaatttgt ggaatggcct tccgacagtt ttccaacagc
     6721 cgagacaccc attatcatcg gggttttggg cacaaaccct ttcggcggcg acttggacgc
     6781 ggtcatccac aacaagactg tcaacaatcg cccgatagaa atcagacaac tgcatctaat
     6841 gagcgaatgc actaactgcc atgtcctttt catcagtggg atggtaaaaa aacgggtggg
     6901 ggaagtgcta agtacggtgg ggaacgcgag tgtcctgact gtcggcgaat cggacggttt
     6961 taccgaggcc gggggaatca ttaattttgt tcgcgagggg aacaaatttc gcttccaaat
     7021 caatgccgaa gccgccaaaa aggcaaagtt gaaaatcagt tccaaactgc tgggcttggc
     7081 tttgccatcg aattccaatc actgaaccat ttgcttgctt ggatttgcag gtttctcaat
     7141 caaaggggca tacatttatt cttaaagttg accttggcac gtggattgca gtctttctgc
     7201 gccagcatga ctacatgccg aaaggtaaat aaattgaccg gcaatcgtcc gattccgggg
     7261 cagggcggcg ttttgccgtc agtggtcgcc tcatctggcg cagagtcaac gagttagagg
     7321 aagctcttga tcaaaccttt ctgaaccgat gaaattgttc cgcgatctgc cgattcaaca
     7381 gaagacgttg gtgatgacgc tctccatttg tggagcggta ttgtgcatcg ccatcgcggt
     7441 gttgttcact tttcaggtcc tcaactttcg cgcgaattat cagcgcgacc tggatactgt
     7501 ggccgtcatc atcgcgaaca acggcaccgc agccctggcg ttcaaagatg acaaggcggc
     7561 aacggaattg gttgactcgc tccgcgccaa accaagcatt ctgacagcga gtcttctgct
     7621 gccggatggt tccatgttcg tccattacgg gcggcctgag aacgcggcaa aattggcgca
     7681 attcccgccc gccggacaac aactgtttgt gggaggcgat ctgctcctca cccaaccggt
     7741 cgaattgaag ggcgaacgcc ttgggaccct ttacatcagg gcggattatc accggacttt
     7801 tctggaattg ctgcacgttt atggattggt cattcttggg attgttgctg cctcgatcgg
     7861 cttggcggcg tttctctcgg ccaagctggg caaatccatc acacagcccg ttctgatcct
     7921 cgcgcaagcg gcacagatcg tcggcgaaaa gaaggattac tcggttcgag taccggccag
     7981 ttcgcagggc gatgaactcg gtcaactgac gcgcgctttc aatgagatgc tcagccgcat
     8041 ccagtcgcag gacgctgcgc tcagtctgtc gcaacaaaag atggaggcct tgatccattc
     8101 gattgacggc attgtgtggg aaagaacggc tgacgcgcac aaatttactt ttgtcagccg
     8161 tcaaagcgaa gaaattctcg gctacgcgcc cgcgctctgg tgcgaaaagg aggatttttg
     8221 ggaatcgaaa ttgcacccac aggacgccgc caaggcccgg cagacgaatc gagagatggc
     8281 cgcgcgcggg cagccttaca gctacgaata tcggatgctc gcagcagatg accggactgt
     8341 atggatccgc gaaagcggca ccgtgctgat ggaaaatgga aagccaatcg caatgcgggg
     8401 cattttccag gatataaccc agagcaagct ggatgcgcaa aatctcgaca agctcaaccg
     8461 ccagttaatg gatacttcgc gtcaggcggg catggcggaa gtcgccaccg gcgtgttgca
     8521 caacgtcggc aacatcctga acagcgtcag tgtttccgcc acgctggcag gcgacaaatt
     8581 gcgccagtca aagattggaa atttgcgcga agcgactgcc ttgttgaacg agcagaacgg
     8641 ccatttggtt gaatttctga cgaacgatcc cggcggcaag gtgctgccga cttatctgtc
     8701 cgcgatggca gatcaaattg cgggcgaaca agccaatctc ctcaccgaaa tctcctcgat
     8761 caaccaaaac gtcgagcata tcaaggaaat cgtcgcgatg cagcagagtt acgcgaaggt
     8821 ttccggcgtc tatgaaaatc tgtccgtcgc cgggttggtc gaggatgcct tgcgcatgaa
     8881 catcgccgcc ttcgaccggc attcgattga ggtcatcaag gaattcgatg aaaacatgcc
     8941 ccatgcgtcc gtggaccggc ataaaatttt gcaaatcctc atcaaccttc tgcgcaacgc
     9001 gaaacatgcc atggatcatc gccctgctag tgagcggcag atgcgcatcc gcctgtgcat
     9061 ggcttcgcct gaccgcgtca agatcagcat caaggacaat ggcataggca tcgcgccgga
     9121 aaacctcacg aagattttta accacggatt caccaccaaa aaggacggtc atggttttgg
     9181 gcttcacagc ggagccaacg ccgccaaaga aatgggaggc agcctctctg cccatagtca
     9241 tggacttggc catggcgctg aattcactct tgaactaccc accgcagtcc ccgggcgcaa
     9301 ggaaggggaa cagaccacaa ccgccgtttc gttatgaaca gcaagccgct cgaacccaac
     9361 cgccgcatcc tcatcattga tgacaatgcg agcatccatg ccgatttcaa aaaaatcctt
     9421 tgcccggtgg attcgaacaa ctccgaagtg aacgagatgg aggcgttgat tttcaagaga
     9481 gcgaaaaaaa atatttccca gcacgtattt catttggatt ccgcttttca gggacaggaa
     9541 gcgttggaaa tggtcaagca atccctcaag gatggacggc cttatgccac ggctttcgtt
     9601 gatgtgcgca tgccgcccgg ctgggacggc attgaaacca ttgcgcgcat ttgggaggtg
     9661 gatccgcaga tccaaatcgt gatctgcacc gcttatgcgg attattcctg ggaagaaatg
     9721 cgggggaagg tgggccaacc ggacagcctg ctcgtcttga aaaagccatt cgacaacatc
     9781 gaagtccagc aaatggccca tgcgctcaca aaaaaatggc ttttgaattt tcaaacggtg
     9841 cagcaaatgg cagatctcac ccaagccaac aaagcgctgg cggaattgga ggagcggttc
     9901 tccaaggcgt ttcatgaaag cccggtgccc tccggaatcc agagcttgcc cgataaacgg
     9961 tttgtggacg tgaaccatcg tctcgcacag atcgccgggt tgcgacggga agacatgatt
    10021 ggccgctccc cggcggaatt atttctctgg gaaaaacctg ctgccgccga acaatggtat
    10081 gaaagcgcca ccggccggca attggtgcgc gcgcaggaag gcaaactccg cgagaaaggt
    10141 gggcgactgc atgaagtgct cgtgtcgttg tgtccgctcg ccttgggcga aaagtcgcac
    10201 ctgcttctgc tggccgagga tgtttccgag caggcgctcc ttgaacgcca attgcgccag
    10261 tctcaaaaga tggaggccat cgggcaattg gccgcgggcg tcgcccatga tttcaacaat
    10321 attctcacgg tcatacaggg tcatacaggc atgatccaac tccagatgga acccaattcc
    10381 ccgctcagaa aatctttgga gcagatcagt ctttcgaccg agcgcgccag cgcgctcatc
    10441 cggcaactgc tcatgttcag ccgcaagcag gtgatgcagt tccggcacgt ggacctgaac
    10501 aacactttgc aaaacgtgct caagatgctg gagcggttgc tgggcgaaac cgtgcaggtc
    10561 gagtttcatc cgcagccgtt catgcccgca atttacgccg acaccagcat gttggagcag
    10621 atcgcgatga accttgccgt caatgcgcgc gatgccatgc ccaacggcgg gtttgtcaaa
    10681 atccttacct cgatgcaaac catccggcgc ggtccgaacg cgctggacgc gcaggcccgc
    10741 gatggcgaat tcatttgcct cacgttcagt gacaacggca cggggatgga cacgcagctc
    10801 atcaaccgga ttttcgagcc gtttttcacg accaaaccgg ctggcaaagg cacgggtctt
    10861 ggcttgtcaa cggtcctcgg catcgtgcag caacacagcg gctgggtcga agttgaaagc
    10921 aagccgggga aaggcaccac gttccgtctt tattttccaa aaagcgagcg gcccgcggaa
    10981 aagcttgaaa agattgagga aaccgaactc ctgaaaggac gcgagacggt gcttgtcgcc
    11041 gaggacgaag gtgccttgcg cgaaatggtg gcgttggtgc tcaggacgca gggctacacg
    11101 gtcttggaag cggcatcggg ccgcgaggcc attgagaagt ggggtcgttc agagcgcaaa
    11161 gtcgatttgc tggtgaccga catggtcatg ccaggcggcg tccttggcgg cgaactggcc
    11221 gaatgccttc aggagaaatg ccccagcctc aaggtgatat acaccagcgg ttacagtccc
    11281 ggcatggcgg gcaaggacac ctcgctgctg gaaggacgca atttcctgcc gaaaccttat
    11341 tccatcggca aactcgccaa gtttgtgcgc gaatgcctcg acagtccgcc ggtcccgtgc
    11401 tgacctgtga gagctttcga cattctgcac ttaccggggg gaggggggta tttttggcgg
    11461 aattttatga aactttatga aattttgcgg aatctcaagt acttacaact tgagccattt
    11521 cactcgattt caaccaaacc ggatgaaaaa atattttatt tttttatcgc ggtttatccg
    11581 gtcaagccct gttgatctga aaataaccga aacaacctga aacaacgtag ctgaaaacgt
    11641 gagttttcag gtccttccgg gcgggccatg ttcggcgaag tccgccaaac gtccaagcat
    11701 gagagaaccg agacgcgact tgcccgcgaa aaacacgaaa gtccgcgaaa tccgcaactt
    11761 taaacgttaa acctaaacgt taaacttggc ttgccgacgc ggaacccgga actcagaact
    11821 cggaacatta gattgtcaaa gaacagcccg tcgtcatgga gagacgggaa tcagtcaaaa
    11881 tccgcaaagg caggtaaggg gtcacccatc acttagtatt gacacaaaaa tttcatgcca
    11941 attcgtgtcc ttttccttcc gtcttgcata ttgcaaaatg cgacaaattt gtaacaaacc
    12001 ttgcaaactg catcgttttt cgcttcaaac cccacgccag cagcatgttt catctggcac
    12061 gattcaagca gatgaagacc ccattattgg ccgaatgccg ccttgcagca ccggccgttt
    12121 tttgtaaact taacctaact aacgattgac atggatttcc tcatcgttga tgacgacaag
    12181 acttttcgcg acgcaacccg cttgctgatc gaggacgaag gccattacgc ccagcccgca
    12241 gccaacggcg aagccgcgct cgcaatgtta aaagaagaca agttcgacgc tgtccttctt
    12301 gatgtgaacc ttggccccga aaacgggctc gacgtcctca accaactcat caaggtccaa
    12361 cccaaccttc cggtggtcat gttcaccgcg caagggacga tcaagacggc cgtcgaagcc
    12421 atgcgcatcg gcgcgctcga tttcctggaa aagcccttca cccgcgaaca gatgcacacc
    12481 gtcatcgcgc gcctgaaacg cttcagcgtg atgaacgaaa acatccagcg gctggaagaa
    12541 gaggtcaatg aaaccaaact gcaaaacccc gagttgttgc tggacttcgc cagtcccggc
    12601 atgcgccaga taatggaaat ccttcagcgc gcagccagga cgccggcatc gattctcatc
    12661 actggcgaaa gcggcactgg caaaagcgtc cttgcccggg ccgtccacga gcaaagccat
    12721 cttgccgaca agccgttcgt cacggtgagt tgcccgagtt tgtcgaagga attgttggag
    12781 agcgaacttt tcggccatgt caaaggcgca ttcaccggcg cggtcaaaga ccattggggc
    12841 aaggtgaagg ccgctggcgg cggaacgctt ttcctggatg agatcggcga tttgccgatg
    12901 gaaatccaac ccaagctgct gcgccttttg caggagcgcg aatacgaacg cctcggcgaa
    12961 aacatcacgc gccaggctga gttgcgcatc atcgccgcga caaaccgcga tttgaaaaag
    13021 cgcatgaccg aaggcgcatt ccgcgaagac ctttatttcc ggctcaatgt catcgccgtg
    13081 gaaatgccgc ccttgcgcac gcgccacgac gacatgctcc gtttcgccga gcattacctc
    13141 aaatattttt ccaaacaatg cgcgcgtccc atgccgaaga tttccgggcc ggcggccacg
    13201 cacatgctgg cttatacgtg gccgggaaat ttgcgcgaat tgcgcaatgc cattgagcgc
    13261 gccgtcatcc tcgccaaggg cgacgaactc tgcgctgaag atttgcccgg cgacctcgac
    13321 ggcgattcca atcaccgcga tgcggacctt caacccggcg cattgatctc gctcgaacgc
    13381 ttggaggaac tccacatgcg caaagttttg gagcgcacct ccaccgtcac cgaagccgcg
    13441 cacattttgg gcatcgacca agccactatt taccgcaaga ggaaaaaatt ggggatgagt
    13501 taaggtccaa agccaaaggg ccggtcaaat catggcgttg ctgggcaaaa aagggaatga
    13561 cacgaattcc actaattttc acaaatttaa aaccattgct ccgcaaattc gtgtcaattc
    13621 gtgtaattcg tgtctcttct tctccgttta ttccgtgttc atctatgtgc atccgtggct
    13681 tccgttctct tttcgtatag aatgcagctc tgcctgcgca gaaaagcacc tgtttttctt
    13741 gtggctccaa taacggttgc cggctgaccg actcagaaca tgcgatccaa cggagaactc
    13801 tttttagccg ggtgccaaaa agtcttggca tcgcgttgcg gtttgagttg caagaggtct
    13861 ttgcccagca agcgcaacac caggccgaac ggcgtcacca ccaggacgaa taatgcagcc
    13921 aacacaatgc ggccgagcaa ttgggtgatg ccgaatccaa cccggctggt gaaccgatag
    13981 tacccgcgaa accaacccgg acgcagccat gcgaaaacgc tcaacagcgc caaaccaatc
    14041 agcgccgcca tccagcccct cgtcggcagg atatgccgcc agcgcaacac cgttgtcatc
    14101 ccagcgaatc ccagcgcgcc gagccatgcc gccttgcgcc attccttcgg atcttctttc
    14161 agtttcagtc tcatgtttaa tccgcttgcg gcgcaacgcg tcgttcagcc gtttgtttcg
    14221 gctgcgcagt ccgttcgatg acaaaacttc cgatgaccaa gtaatccatt tccgtgttca
    14281 cgaagcagcg atacgcatcg tcgggcgtgc aaacgaccgg ttcgccgcgc acgttgaacg
    14341 acgtgttcac caaaactccg cagccggtcg ccttttcgaa ccgtagcagc aaatcataca
    14401 gcagcggatt gtccgtttgc gcaacggtct ggatgcgcgc ggaataatca acatgcgtga
    14461 ttgcgggaag agtggagtgc tcggctttca agagttccag tcccgtgacc ccgggtggca
    14521 ctggccgtcg aagctccgct ttgatcggcg cgaccaacag catgtaaggc gattcctggt
    14581 cgagttcgaa ataatcggcc acgcgttcgc gccgcaccac cggcgcaaac ggacgaaacg
    14641 attcccgaaa ctttactttc aaattcatca ccgattgcat ctgcggcgaa cgcgcatcgc
    14701 cgaggatcga gcgattgccc aaggcgcgcg gaccaaattc catgcggccc tggaaccagc
    14761 ccaccacttt cccggcgcgc aacagaccca cggtcatgtc gagcaaagca tcgtccgaca
    14821 aacgctgata aaccgccttg ttgctttgca gcacggcttc gatttccgcg tccgaatatt
    14881 ccggaccaag aaacccgccg cgcattgcat ccgttttggc aggacggcgg gtttcgtcag
    14941 gttgcgacgt tttgtccaga tgccatgcgg tcaatgccgc tcccaatgcg ccgccggcat
    15001 cgcccgccgc gggttggatc caaagccggt caaaaatttt ctcgcgcaag atgcgcccgt
    15061 tggcgacaca gttcagcgcg acgccgcctg ccatgcacaa atctttcagc cccgtcactt
    15121 cgcgcgcgcg atgtgccaag cccagcatga tttcttccgt gaccacctgg atggaccgcg
    15181 ccacgtccat gtgccggcgt tcaatcggtt cttccggccc gcgcggtcca ccgccgaaca
    15241 gccggtgaaa acgcccgttc gtcatcgtcg tccctcccag aaaatcgaag aaatcgaggt
    15301 tcagacggaa cgaaccgtcg ggcttggatt cgatcaaatt ctggcggatg gcatccgcgt
    15361 atttgggttc gccatacggc gcaagcccca tcagcttgta ttccccggaa ttgatgcgaa
    15421 acccgcagta ggacgtgaac gccgaataca gcaatcccaa cgaatgcgga aaccgcagtt
    15481 cttccaacaa ctggatttcc tggccgcgcc cgacgccaat cgtcgtcgtc gcccattcgc
    15541 ccacgccatc aatggtcagg atggcggctt cggca
//
LOCUS       DASZMM010000325         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4523944, whole genome shotgun sequence.
ACCESSION   DASZMM010000325 DASZMM010000000
VERSION     DASZMM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4523944"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VG754_07870"
     CDS             complement(<1..733)
                     /locus_tag="VG754_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF19168.1"
                     /translation="MTSHFQFQFPWVLALLALLPVYAFLRGRIGNMSALRFSSADIAR
                     AAGGAARAAAGRLLLFMRLLVLALCIVALAGPRFANDRTETQASGVDIMLVLDLSWSM
                     ESLDMGGTTEETTRFGIASSVLEDFIGKRPNDRIGLIAFSGVPYLASPLTLNHEWLAQ
                     NLQRLHVGTIRELGTAIGDATAAAAKRLEMLKGSKSRIIILLTDGDNNQGEIDPVPAA
                     QVAAAIGAKIYTIGIGIEEPCRLPAF"
     gene            complement(733..1275)
                     /locus_tag="VG754_07875"
     CDS             complement(733..1275)
                     /locus_tag="VG754_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 family protein"
                     /protein_id="HWF19169.1"
                     /translation="MACFIFLVNAARGQGHELPPLQPPHGDLAPTFWEQHGWQVVLAA
                     AFCVLVIGWIIIWLRRPKVFIPEPPAIVARRALESWRGQAQDGALVADVSRVLRRYVM
                     AAFNFPSEELTTTEFRKALQSHSQIAPDIASATGDFLRRCDEWKFAPTPPAPQLGAVA
                     VALELVNKMEASRAPVPVKT"
     gene            complement(1521..2438)
                     /locus_tag="VG754_07880"
     CDS             complement(1521..2438)
                     /locus_tag="VG754_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWF19170.1"
                     /translation="MTINEILETVRRVEIRTNRLVNDMMVGAYLSRFKGRGMDFEELR
                     EYMPGDDVRDIDWNVTNRMGRPFVKRFREERELGVILAVDVSASSAFGSARLSKREAA
                     VEIATTLASSAARSSDKVGLLLFTDEVELFLPPRKGRRHILRTIKEMLFFKPKHRGTN
                     IPAVLSFLNHVIKRRSIVFLFTDFLHSFGADARSVRAGRELLQRIGMTNSRHDLVCVH
                     LHDPRESKLPSAGLLTIEDAETGEMLELDSMRAGVRQTYARANAERLGELERALRHAG
                     VDTLRFSTGEPFAQVLQSFFETRRGRRRG"
     gene            complement(2529..4421)
                     /locus_tag="VG754_07885"
     CDS             complement(2529..4421)
                     /locus_tag="VG754_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19171.1"
                     /translation="MTVQETPEASPRESVTSLAGRNWVRWMAVSVSFIVLLGWVYSAA
                     VYSNYGFSDDYQLLKRVGEGSFDLRHNEMVQGGRPILGLGIKLLFSIPHSIDSLKYAR
                     SVPIAGILLLALVAFVIFKANQFDDAFSVTSAFLICLTPGIAVYAGWLSAGLFVYAAV
                     LAFPAGHLFNLAIDEAKFSQRAIRFCLGGLLLLITYTIYQPAAAFAVIPMLARMWRGF
                     ELNRERLRGFTIGMGMLVLTAAIYFVGFKIFVAVFHPGGQAARGEFTKDILGNLRFVV
                     TQVLWSGVTGWFQFHSTAAQYTAAFVVIIVIVGGCVIRPWKSFAIQMCGLACVVTAVF
                     FTVLNLLLLKEQIFAFRLQTALHGAIALMFMVGLERWLRMFSPTHATKVRAVAFALLI
                     CSAAILTRYHFCNDFVIKNKRELSALRRGVAGWKDFPKILVFITPAQAVMPGDSIGEF
                     RDLTVSWSWVAPSMLELLFIETLERGVPLHEALNRHATFSVNYGPPNPDSLPVLNGFA
                     LVNGEPSVIHDPYWGDIHTNRNGWCYADWFGVFDNRRFPAIYHAQLGWLQCSGKGGDD
                     FWFGNQKNGWFWTTPKSYPYLMFKDSGKWFFLSPGFSFEPNFCEMESKQWTTAPQSFA
                     PATKKQ"
BASE COUNT         1181 a         1419 c         1223 g          929 t
ORIGIN      
        1 caaacgcggg caggcggcaa ggctcttcga tgccgatgcc gatggtgtaa atcttcgcgc
       61 cgatcgcggc agcgacttgc gcggcgggaa cggggtcaat ctcgccctgg ttgttgtcgc
      121 catcggtcag caggatgatg atgcggctct tgctgccttt gagcatttcc aggcgcttgg
      181 ccgcggcagc cgtcgcatcc ccaatcgccg tgcccaattc gcggatggtg ccgacatgca
      241 gacgctgtaa gttttgcgcg agccattcgt gattgagcgt caatggactt gccaggtacg
      301 gaacgccgga aaaagcgatc agcccgatgc ggtcgttcgg gcgcttgcct atgaaatctt
      361 ccaaaacgga actggcaatg ccgaagcgcg tggtttcctc ggtcgtgccg cccatgtcca
      421 gcgattccat ggaccacgac aagtcgagca ccagcatgat gtccacaccg ctcgcctgcg
      481 tttccgtgcg gtcatttgcg aaacgcggcc cggccagcgc cacgatgcag agcgccagca
      541 caagcaatcg catgaacaac aacaaccgtc ccgccgctgc ccgtgccgcg ccgcctgccg
      601 cgcgcgcaat gtccgcgctc gaaaatcgca atgcggacat attcccgatg cgtccgcgca
      661 aaaatgcata caccggcagc aacgccagca gcgccagcac ccagggaaat tgaaattgga
      721 aatgcgacgt catcaggtct tgactggaac cggcgcgcga ctcgcttcca ttttattcac
      781 caactccagc gcgactgcga ctgcgccaag ttgtggcgct ggtggcgtcg gcgcaaattt
      841 ccattcatcg catcggcgaa gaaaatcgcc ggttgcagag gcaatatccg gcgcaatttg
      901 cgaatgcgat tgcaacgctt tcctaaattc cgtggtggtc agttcctccg aaggaaaatt
      961 aaacgccgcc atcacatacc gccgcaacac acgcgaaaca tcggccacca gcgcgccatc
     1021 ttgcgcttga ccgcgccagc tctccaatgc gcgcctcgcc acgattgcgg gcggctcggg
     1081 aatgaaaacc ttgggacgac gcagccaaat gattatccac ccgatcacca gcacgcaaaa
     1141 cgccgccgcc aataccacct gccatccgtg ctgctcccaa aaagttgggg ccaaatcacc
     1201 atgcggcggt tgcagcggcg gcagctcatg cccctgccct cgcgcggcat ttaccagaaa
     1261 aataaagcac gccattactg cgcttgccga tgaacccgcg gatcgaagtg acagcggaac
     1321 gcgagttcct gtagcgcagg cttgcagcct gctgtatcgc cgaattgcac tcggcattgc
     1381 gaaagaaaaa tcggagcggg cacgactttt tatgacccgg cagagtggaa ctctgcgata
     1441 cagcagagta aaactctgcg ctgccaatcc ggcgcatgct tgccgcaacc aagctaaaac
     1501 cttcaacctc ggacctcgcc tcatccacgc ctcctgccgc ggcgcgtctc aaaaaacgat
     1561 tgcaacacct gcgcgaaagg ttcgccggtg ctgaaccgca gcgtgtccac acccgcatgg
     1621 cgcaacgcgc gttccagttc gcccaaccgt tccgcattgg cccgggcgta agtctgccgc
     1681 accccggcgc gcattgaatc cagctccagc atttcgcccg tttcggcgtc ctcaattgtc
     1741 agcagcccgg cgctcggcag cttgctttcc cgcggatcat gcagatgcac acaaaccaga
     1801 tcgtgcctgg aatttgtcat gccgatgcgt tgcaacaatt cgcgtccggc gcgcacactc
     1861 cgcgcatctg cgccgaagct atgcaaaaaa tccgtgaata aaaaaactat cgaacgccgc
     1921 ttgatgacgt gattcagaaa tgacaacacc gccgggatgt tcgttccgcg atgtttcggc
     1981 ttgaagaaca acatctcctt tatcgtgcgc aaaatgtgcc gtcgcccttt gcgcggtggc
     2041 agaaataatt ccacctcgtc cgtgaacaac agcaaaccga ccttgtcgct gctgcgcgcg
     2101 gcggaagagg cgagcgtcgt tgcgatctca accgccgcct cgcgtttgct gagccttgcg
     2161 gagccaaacg cgctcgatgc cgaaacatcc acggctaaaa tgacgcccaa ttcccgctcc
     2221 tcgcggaacc ttttcacgaa cggcctgccc atgcggttgg tgacgttcca gtcaatatcg
     2281 cgcacgtcat cgcccggcat gtattcgcgc aattcctcaa agtccatgcc gcgccccttg
     2341 aagcggctga ggtacgcgcc gaccatcatg tcgttcacca gccggttggt gcggatttcc
     2401 acccggcgga cggtttcaag aatttcgttg atagtcattc agacgcgttt atatttggca
     2461 tccaacgtaa gccaaccatg cccattccga caatggcaaa aggaattttt gcgacgcctg
     2521 aagaacaatc attgcttttt cgtggctggc gcaaaggatt gcggagcggt cgtccattgt
     2581 ttgctttcca tctcgcaaaa attcggctcg aaggaaaaac ccggcgagag aaaaaaccat
     2641 ttgcccgaat ctttgaacat caagtaagga taagatttcg gggtcgtcca aaaccaccca
     2701 tttttttggt tgccaaacca aaaatcatcg ccgcccttgc ccgaacattg cagccagcca
     2761 agctgcgcgt ggtaaatcgc cgggaaacga cgattgtcga acacgccaaa ccagtcggca
     2821 taacaccatc cgttccggtt ggtgtggatg tcgccccaat aggggtcgtg tatcacggaa
     2881 ggttcgccat tgaccagcgc aaacccgttc agcaccggca aagaatcagg gtttggcggc
     2941 ccataattga ccgaaaatgt cgcgtgacga ttcaacgctt cgtgcaaggg aacgcccctt
     3001 tccaacgttt cgatgaagag caattccaac atggatggcg caacccagga ccatgaaacg
     3061 gtcaagtccc tgaattcccc aatactgtcc ccgggcatga ccgcctgcgc aggcgtgatg
     3121 aaaaccaaaa tttttgggaa atccttccaa ccggcgacgc cgcgccgcaa tgccgaaagt
     3181 tcacgtttgt ttttgataac gaaatcattg caaaaatggt agcgggtcaa aatggccgcc
     3241 gagcaaatca acaaggcaaa cgcaaccgcg cggactttcg ttgcgtgggt tggagagaac
     3301 attcgaagcc aacgctccaa tccgaccata aacatcagcg caatcgcgcc atgaagcgct
     3361 gtttgcaggc gaaaagcgaa aatttgctct ttgaggagca ataaattcaa aaccgtgaaa
     3421 aacaccgctg ttacaacaca ggccagtccg cacatttgga tggcaaaact tttccatggc
     3481 ctgatcacgc aaccgcccac gatgacaatg atcacaacaa aagcggctgt atattgcgcg
     3541 gctgttgaat ggaattgaaa ccagcccgtc acgcctgacc agaggacttg cgtcacgaca
     3601 aaccgcagat tgcccaggat gtccttggtg aattcaccac gcgctgcctg tccaccggga
     3661 tgaaaaacgg cgacgaaaat tttgaaacca acgaaataaa ttgccgccgt caaaaccaac
     3721 attcccatgc cgatcgtaaa acctcgcaag cgctcacgat tcagttcaaa accgcgccac
     3781 atgcgcgcaa gcatcggaat gacggcgaaa gccgctgccg gttggtaaat ggtgtatgtg
     3841 atgagcagaa gcaacccacc aaggcagaaa cgaatggcgc gctgggaaaa tttcgcttca
     3901 tcaatcgcaa gattgaacaa atggccggca gggaacgcga ggaccgccgc ataaacaaac
     3961 aagcccgccg acaaccaccc cgcataaact gcaatccctg gcgtcaggca tatcaggaat
     4021 gcgctggtga cggaaaaagc gtcgtcaaat tggttcgctt taaagatcac aaacgcgacc
     4081 agagcgagaa gcaggatgcc ggctattgga acagaccttg cgtacttgag ggaatcaatc
     4141 gagtggggaa tggaaaacaa aagtttgatt ccgagcccca atatcggacg gcctccctgc
     4201 accatttcgt tatgccgcaa gtcgaaagag ccttccccga cccttttcag cagttgataa
     4261 tcgtcgctga aaccgtaatt ggaatacacc gccgcggaat aaacccagcc caagaggaca
     4321 ataaacgaga ctgaaacggc catccaccga acccaatttc gtccggccag actcgtcacg
     4381 gactcacgcg ggcttgcttc cggcgtttcc tgaacggtca taaatcgaat ctgtcttttg
     4441 tcttttgcat tcagcttgaa acatgagtga tggattgcgg tgctgtcaat cccgcaacct
     4501 gcaatggcaa tgagaggggc gtatctggat actgttctta aatctattgt gagaagcgag
     4561 agagggctcg ccctcatccc ggcctaatgg cttcccaagc ccgccacaag tcagtttccc
     4621 caaaagagac gaacgcttcg cgttgtgtgg tccccctggg agaaggagaa ccggcatccg
     4681 cccatttatt attggccgcg atggtttgca ccattccgtc acgagaatgt tccgaacagc
     4741 gggaaatggt ga
//
LOCUS       DASZMM010000326         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_702323, whole genome shotgun sequence.
ACCESSION   DASZMM010000326 DASZMM010000000
VERSION     DASZMM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702323"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VG754_07890"
     CDS             complement(<1..351)
                     /locus_tag="VG754_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19172.1"
                     /translation="MSDSTEKTFEQASAFQKIWMETFTKMAQSGFSFSPESAPPEVLR
                     QMRSGIFHALAKSWEEFMRSPQFLDSMKTMMDNAINFRKMSNDLLTHAHHSIQGTARA
                     DIDDLMQAIHHLETR"
     gene            complement(493..1671)
                     /locus_tag="VG754_07895"
     CDS             complement(493..1671)
                     /locus_tag="VG754_07895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWF19173.1"
                     /translation="MSKNIYLAGAARTAIGSFCGAFESLPAPALGGIAIHAALERSGV
                     PANKVDEVIFGNVISAGLGQNVARQSAIGAGLGPEVGATTVNKVCGSGLKAVMLAAQA
                     IQCGDAGVIIAGGTENMSRAPYLLDRARTGYRLGNGELIDSVIKDGLWDVYNNLHMGV
                     CGDRCAQKYVFTRQQQDDYAVESFKRALAATSQCVFSKEIVPVEVPGGKEPVVVKEDE
                     TLKKFNEEKLRKLRPAFGKEGTITAGNASSINDGAAAIAVMSEERMKELGVKPQAKIL
                     GYATFSREPEWFTIAPVGAISRLLEKLSLKVQDIGLFEINEAFSVVPMAAMKDLNIPH
                     DKLNVYGGAVALGHPIGASGARTLVTLLNAMKHRGAKIGINSLCIGGGEAVAMAVELC
                     "
     gene            complement(1726..2265)
                     /locus_tag="VG754_07900"
     CDS             complement(1726..2265)
                     /locus_tag="VG754_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19174.1"
                     /translation="MKTREKGHSGHSRPHSEPNNASAPGAEMFNQAVKGYEQALRTSA
                     RLQEDAARWWTNVFQQSGYTQDWQRQMNSVVSEAIPTAERNLEESLRLVDQSCKTGLN
                     LLKRAVNAPRSNGTSDVQSQMQDIFQSSLNVFHSNLQAITESQARVMESWTEFMRKGI
                     DSTASTAASAASSATAAAK"
     gene            complement(2515..3147)
                     /locus_tag="VG754_07905"
     CDS             complement(2515..3147)
                     /locus_tag="VG754_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HWF19175.1"
                     /translation="MAEANAPHSKPLDIRKYPNRRYYDVTRSQHLTLEDIRNLVREGH
                     DIRVTDSKTSTDITAKVLAQIILELDAEKIQTFPVAMLSRLIRVNDQMAKDFLERYFD
                     QAWTTLTKYRQQFEEQFGAGAGLGSFYSSLADWNRAMLNPFGMPFMGGKPASAEAEKN
                     APRSDTQKSDLQDKVSTLQQQIARLEQKLAKKQQVGKKRPRSATKRHKSL"
     gene            3436..>3730
                     /locus_tag="VG754_07910"
     CDS             3436..>3730
                     /locus_tag="VG754_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF19176.1"
                     /translation="MNIKGKVAVITGAAGGIGRAVAIELAKRGAGHIALVDKTEAVHA
                     LATEISKETGASTATGYTGDVTDDTFRSWVYEDIREKRGIVQICVPAAGITRDG"
BASE COUNT          765 a         1024 c         1027 g          914 t
ORIGIN      
        1 gcgtgtttcc agatggtgaa tcgcctgcat caggtcgtcg atgtccgcgc gcgccgttcc
       61 ctggatgcta tgatgcgcgt gcgtcagcag gtcgttgctc atcttgcgga agttgatggc
      121 gttgtccatc atcgtcttca ttgagtctag gaattgcggc gagcgcatga attcctccca
      181 tgatttggca agcgcatgaa aaatgccgct ccgcatctgg cgcaacactt ccggcggcgc
      241 agactccggc gaaaatgaaa acccgctttg cgccattttt gtgaaagttt ccatccagat
      301 cttttgaaaa gcgctggcct gctcgaaagt tttctcggtg gagtcgctca ttgttttggc
      361 gttttggccg ggaggatcag acgctcccgg ttgttcgttg ttcacttact ctggcatcat
      421 tttcccgacg gtcaagctgt gttgaccgtt tacaaaggga cggcgcccgc gtcgtccccg
      481 cacggttggc aatcagcaaa gttcaactgc cattgccaca gcttccccac ccccgatgca
      541 gagcgagttg atgccgattt tcgcgccgcg atgtttcatt gcgttgagca aggtcaccag
      601 cgtccgcgcg ccgctcgcgc cgatgggatg acccagcgcc accgcgccgc catacacatt
      661 caacttgtca tgcggaatat tcaggtcctt catggctgcc atgggcacca cggaaaacgc
      721 ctcgttgatc tcgaataatc caatgtcctg cactttgagc gagagctttt ccaggagcct
      781 gctgatggcc ccgacgggcg caatggtgaa ccattccggt tcgcgcgaaa acgtggcgta
      841 accgagaatc ttcgcctgcg gtttcacgcc aagttccttc atgcgttcct cggacatcac
      901 cgcgatcgcc gccgcgccgt cgttgatgct ggaagcattg ccggcggtga tggtgccttc
      961 cttgccgaag gccgggcgca gcttgcgcaa tttttcctcg ttgaatttct tgagggtctc
     1021 gtcctccttc accaccacgg gctccttgcc gccgggaact tcaaccggga cgatttcctt
     1081 tgaaaaaacg cattgcgaag ttgccgcgag cgcgcgcttg aaactttcca cggcgtaatc
     1141 gtcctgctgt tgccgcgtga aaacatattt ctgcgcgcaa cggtcgccgc agacgcccat
     1201 gtgcagattg ttgtaaacgt cccacaatcc gtccttgatg actgaatcaa tgagttcgcc
     1261 gttgccgagc cggtaaccgg tgcgcgcgcg gtcgagcaga taaggggcgc gggacatgtt
     1321 ctccgttccg cctgcgatga tcacgcctgc atcgccgcac tggatggcct gcgcagcaag
     1381 catgacggcc ttgaggccgg aaccgcagac tttgttgacg gtcgtcgccc cgacttcagg
     1441 gccaagtccc gcgccaattg ctgactggcg ggcgacattc tggccaaggc ccgcgctgat
     1501 cacgttgcca aaaatgactt cgtccacctt gttcgcggga acgccagagc gttcgagcgc
     1561 cgcgtggatc gcgatgccgc ccaacgcggg cgcgggcaag gactcaaatg cgccgcaaaa
     1621 actgccgatg gcagtgcgcg ccgcgcctgc tagataaata ttcttcgaca taacaattaa
     1681 ctttctggtg tgctctgttg gaggacaagg gcaccggttg cattgtcatt tcgccgccgc
     1741 agtggctgac gaagccgccg atgccgcggt cgatgcagtt gaatcaatgc ctttgcgcat
     1801 gaattccgtc cacgattcca tcacgcgggc ctgcgattcg gtgatcgcct gcaggttgga
     1861 atgaaacaca ttcagcgagg actggaaaat atcctgcatc tgtgattgca catcggaggt
     1921 tccgttggag cgcggcgcat tcacggcgcg cttgagcaga ttgagcccgg tcttgcagct
     1981 ctggtccacc aaacgcaagg actcctccaa attccgttcc gcggtcggga tggcctcgga
     2041 aaccactgaa ttcatctgcc gctgccagtc ctgcgtgtaa cccgattgtt gaaaaacatt
     2101 tgtccaccac cgggccgcat cttcctggag acgcgcgctg gtgcgcaatg cctgttcgta
     2161 gcctttcacg gcctggttga acatttcagc gcctggtgcg ctggcgttgt tcggctcact
     2221 gtgcggacgg gaatgtcctg aatgtccttt ttctcttgtt ttcatagtcg tagtcctttc
     2281 agttgctggt ttttttcagt gtcgttttgt tttcagttcc ggattccagc ccgccctgat
     2341 tcacaatagt tgtgcaattg cacaattcgt gagaaattaa atccctcatg ggctgaagtg
     2401 caagttttca tcttctttgc cttaaacagg ctttgaaacc aagctgcatt gtaagagtag
     2461 aaccaggcaa ataccttgtc aagaattttg ttgtgcaatt gcacaacgac tattttacag
     2521 gcttttatgg cgtttggtcg cgcttcgcgg ccgttttttg cccacttgtt gctttttggc
     2581 cagtttctgc tccagcctgg cgatttgctg ctggagggtg gaaactttgt cctgaagatc
     2641 ggatttttgc gtatccgaac gcggtgcatt tttttccgct tcagcgcttg cgggcttgcc
     2701 gcccatgaac ggcataccaa aaggattcag catggcccgg ttccaatcgg caagcgacga
     2761 atagaaggaa ccgaggcccg ccccagcgcc aaactgctct tcaaactgct gtcggtactt
     2821 ggttaacgtc gtccaggctt gatcaaaata gcgttccagg aagtccttgg ccatctggtc
     2881 gttgacgcgg atgaggcgcg ataacatggc gaccggaaat gtctgaattt tttcggcatc
     2941 caattcgaga atgatctgcg ccagcacctt cgcggtgatg tctgtcgaag ttttggaatc
     3001 ggtcacgcgg atgtcatgcc cttcgcgcac cagattgcga atgtcctcca gcgttagatg
     3061 ctggctgcgt gtgacatcat aatagcggcg gttgggatat ttccggatgt ccaaaggttt
     3121 ggaatgtggt gcattggctt cagccataag cgtaaaaaaa accctgttgt gcgatggcaa
     3181 taattatccc cgatttgaaa ggaaagtcaa cccggtaata tttcgactgt gcgtggaagc
     3241 gagttctcaa tctatttgta atcagtatca tgcataaatg ccaatttgcg tcacacgtcc
     3301 gcctgtcgaa tgtttttgtc atggtgcgat tgcacaaaat ccattgacta cgcgttcaga
     3361 ccggcttatg gttttgcaat aaccaacaac tcagccgggc cagcccgaaa ccaagaccat
     3421 caaccaacac ctaatatgaa catcaaagga aaagtggcag tgatcacggg agctgccggg
     3481 ggcattggac gcgcggtggc catcgaattg gccaaacgag gcgcgggaca tatcgcgctg
     3541 gtggacaaaa ccgaagcggt ccatgcgctt gccacagaaa tcagcaaaga aacaggcgct
     3601 tcgacggcga cgggttatac cggggacgtg acggatgata ctttccgaag ctgggtttat
     3661 gaggatattc gggaaaagcg cgggattgtg cagatctgcg tgccggcggc tggcatcact
     3721 cgggacggtc
//
LOCUS       DASZMM010000327         4804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1258167, whole genome shotgun sequence.
ACCESSION   DASZMM010000327 DASZMM010000000
VERSION     DASZMM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258167"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VG754_07915"
     CDS             complement(<1..270)
                     /locus_tag="VG754_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HWF19177.1"
                     /translation="MNAGPLLFLATFFTMAISWLGFVLAPQLQLGNQQHVESRTTGDV
                     YPHMRPGMARQGEQVYRANGCNACHTEQVRYKGFGGDYQRGWGGRD"
     gene            complement(267..1733)
                     /locus_tag="VG754_07920"
     CDS             complement(267..1733)
                     /locus_tag="VG754_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWF19178.1"
                     /translation="MSAISSPAQSLPTSDDSFSAPVPAAQIDGSCRAPVLLLLVSAAV
                     WLVISSVFGVIATLKFHAPGLLADCPAFTYGRVHAAALNALVYGFGIQAGLGVLLWML
                     AHLGRTRLAMGPTIIMGALLFNIGMTVGILGILGGDSTGYEWLEMPRYAAVPLFIGYL
                     LIGAGGVVTLHERCERQLSSPQWFALAALLWFPWIYSTAQFLLVTHPVRGALQFALDS
                     WYINNLKTVWFGLTGLAAIFYFISKLTERPLYSHYIGVFIFWTLTLFGSWGAVPPGSP
                     LPAWLPTLSSMGALLTIVPVIAAGINVRQTLKGTCSKMGECRPLKFFWFGSVAYVLSG
                     LAGAVMFLKPVSETLNFTWFIPAQTQLLLYGFVGITMIGAIYHIVPRLLQTEFPSPGQ
                     ICGTFLLAAIGVLFYSVPLAIGGIVQGHALNDAAKPFAEVMNSELLFLRISTLGDFLM
                     AFANLLLLLNLVRILVRAGQTSAAAAWAANSKTSEVRT"
     gene            complement(1753..2133)
                     /locus_tag="VG754_07925"
     CDS             complement(1753..2133)
                     /locus_tag="VG754_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19179.1"
                     /translation="MNPQPECNDCKQGCARCAYFIAVLGAFLIVGGLVWLTYRYTRPE
                     PLGEDRAAVRRKALADLRAANEDILHNPNYVWQDPAKGVVRMPIDRAKELALKMWINP
                     AAARTNLIARVEKATAVAPPPSYE"
     gene            complement(2130..3398)
                     /locus_tag="VG754_07930"
     CDS             complement(2130..3398)
                     /locus_tag="VG754_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19180.1"
                     /translation="MSSQTNSPSSAEPLDLSRWRSLPMWLMIGGAAAVGIGFLVDHDA
                     VTQFAFSWLLAFMFFLTIALGGLGFTILHHLFDAGWSVTIRRIMEQMAYTLRWAWLGW
                     IPLFLFRARLYQWVREIQAGHPDLATKAKLPLFTQPGFVITSLICVGIWWWLSTQLRG
                     WSLKQDETGSVECTRMMRRYSAFGVFLFGLSLTMAAILWVKALEHEWYSTMYGVYIFA
                     GSMWTAVATIYVITLVLDRQGPLKGLVHHKTYYFIGSILFAFTVFYAYVTFAQYFITW
                     NANIPEEGFWYVQRTTGTWWWVCMIIIFGHFFVPFLGLLRIDVKLKYWFMVPLVMWIW
                     LMQFVDMSFNIVPVLHKGGYHLDVMDLGCMALIGGFVARSFLKQLFAHPIAPQRDPRF
                     AESQEIYVPADEYVEAATARGINHKGGGGH"
     gene            complement(3402..4025)
                     /locus_tag="VG754_07935"
     CDS             complement(3402..4025)
                     /locus_tag="VG754_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF19181.1"
                     /translation="MRYILLFFAACTVTVMLIAGKRGTISRRPPIEIFPDMNRQPKLR
                     PQVPNAFFENGRSSQLPVVGTIAREDNYYADTPENTGRIPGTTNFIETIPVTVTAKLL
                     DRGEQRFNINCSPCHGAQGDGKGITVQVGAMPPTPKNLHDKLYVEMKDGEIFNTISNG
                     KGLMGPYGANVTVEDRWAIIAYLRALQLSQLGTVDDVPEQMRSTLKK"
     gene            complement(4025..4564)
                     /locus_tag="VG754_07940"
     CDS             complement(4025..4564)
                     /locus_tag="VG754_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HWF19182.1"
                     /translation="MADTPKTYGIIAEFLNPADTMHAAENVRDKGYRKWDVFTPFPVH
                     GMDKAMGLKNSRVGWFSFLGGVTGYTTGMIMIWWMNAYDYRIVVGGKPMFSPYSAFPP
                     SYELTILFGSFGALLGMLFLNRLPRLHHPLLKHRRFAYASHDRFYLVIETSDPKYSET
                     ETRNLLESLGSLHIELVEE"
     gene            complement(4564..>4804)
                     /locus_tag="VG754_07945"
     CDS             complement(4564..>4804)
                     /locus_tag="VG754_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HWF19183.1"
                     /translation="ERFVIIIMPLHREFIPSNWAYFRPTWVDIMQYLGTFGLFFTMFL
                     LFLRFVPLIAIAEVKGVTPQADPHNPLGGAKGGHH"
BASE COUNT         1187 a         1399 c         1321 g          897 t
ORIGIN      
        1 atcgcgtccg ccccaaccgc gttgataatc tccgccaaat cccttgtagc gcacctgctc
       61 ggtgtggcag gcattgcagc cgttggcgcg ataaacctgt tcgccctggc gcgccattcc
      121 gggccgcatg tgcggataga cgtcgccggt tgtcctcgac tcgacatgtt gttggttgcc
      181 gagctgcaat tggggcgcga gcacgaatcc aagccacgaa atggccattg tgaaaaacgt
      241 cgcgagaaat aacaatggcc cggcgttcat gtccgcacct ccgaagtttt ggagttcgcg
      301 gcccacgccg cggcggcgga agtctgcccg gcgcggacga ggattcgcac aaggttcaac
      361 agcagcagaa gattggcgaa ggccatcaga aaatcgccca gcgtgctgat gcgcaggaaa
      421 agcagttccg aattcatgac ttcggcgaac ggtttcgccg cgtcattcaa cgcatggcct
      481 tgcacaatgc ccccaatcgc aagcggcacg gaataaaaaa gaactccgat ggcggccagc
      541 agaaaagttc cacaaatctg gccgggcgaa ggaaactccg tttgcaacag gcgcggcacg
      601 atatggtaaa tcgcgccaat catggtgatg ccgacaaatc cgtagagcaa cagttgcgtt
      661 tgcgccggaa tgaaccaggt gaaattgagc gtttcactca ccggcttgag gaacatgact
      721 gcgccggcca gacccgacaa cacgtaagcg accgatccaa accagaaaaa tttcaacggc
      781 cggcattcgc ccatcttgga acaagttccc ttcaatgtct ggcgcacgtt gatgcccgcc
      841 gcaatcaccg gcacgatggt caacaacgcc cccatgctgc tcaacgtcgg caaccatgcc
      901 ggcaacggag aacccggcgg caccgcgccc cacgagccaa acagcgtcag cgtccagaaa
      961 ataaatacac caatgtaatg actgtagagc ggacgctcgg tgagcttgga aatgaaataa
     1021 aaaatggccg caagccccgt caatccgaac cagaccgttt tcaggttatt gatgtaccat
     1081 gaatcaagcg cgaactgcag cgcgccgcgc accggatggg tcaccagcaa aaattgcgcg
     1141 gtcgaatata tccacggaaa ccaaagcaac gcggccaaag cgaaccattg cgggcttgaa
     1201 agctgccgtt cgcagcgttc gtgcaaggtc accactccac ccgcaccgat caacagataa
     1261 ccgatgaaaa gcggcaccgc ggcgtaacgc ggcatctcca accattcgta gccggtgctg
     1321 tcgccgccga ggattcccaa aattcccacc gtcatcccga tgttgaacag cagcgcgccc
     1381 atgatgatgg tcggccccat cgccagccgc gtccgcccca agtgggcaag catccacaaa
     1441 agaacaccca aaccggcttg tatcccaaat ccatagacca gcgcattcaa cgcggctgca
     1501 tgcacccggc cataagtgaa tgcggggcaa tccgccagca gtcccggcgc atggaatttg
     1561 agcgttgcga tcactccaaa aacggaactg atgaccagcc agacggcggc gctgacgaga
     1621 agcagcaaca ccggcgcgcg acacgaaccg tcaatttgcg cggcggggac tggcgcggag
     1681 aagctgtcat cgcttgtggg aagcgattgc gctggagaag aaatcgcgct catttaaaaa
     1741 actgcgctgg catcactcgt aagagggcgg cggggccaca gccgtcgctt tttcgacccg
     1801 ggcgatgaga ttcgtgcgtg ctgccgcagg gtttatccac atcttcaaag ccagctcctt
     1861 cgcgcggtcg ataggcatcc ggacgacgcc tttcgcaggg tcctgccaga cataattggg
     1921 gttgtgcaaa atgtcttcgt tcgctgcgcg caaatcggcc aaagctttcc ggcggacggc
     1981 ggcgcggtct tcgccaagcg gctccggacg ggtgtaacga tacgtcagcc agaccagccc
     2041 gccgacgatc agaaacgccc cgagcacagc aatgaaataa gcgcaacgcg cgcagccttg
     2101 cttgcagtcg ttgcattcag gttgcggatt caatggccac ctccgccttt gtgattgatg
     2161 ccccgcgcgg ttgcggcttc gacatattca tcggctggga cataaatttc ctgcgattcg
     2221 gcaaagcgcg ggtcgcgttg cggcgcgatg ggatgggcaa acaattgttt caagaacgag
     2281 cgcgccacga acccgccgat gagggccatg cagcccaggt ccatcacgtc gaggtgatag
     2341 ccgcctttgt gcagcaccgg cacgatgttg aaagacatgt caacgaattg catcagccag
     2401 atccacatca cgagcggaac catgaaccaa tatttcaact tcacgtcaat ccgcagcagg
     2461 ccgaggaacg ggacgaagaa atgcccgaag atgatgatca tgcaaaccca ccaccacgtt
     2521 ccagtcgtgc gctgcacgta ccaaaaacct tcctcgggaa tgttcgcgtt ccatgtgatg
     2581 aaatattgcg cgaaggtcac gtacgcataa aaaacggtga acgcgaacaa tatggaaccg
     2641 atgaaataat aagtcttgtg atgcaccagg cctttgagcg gcccctgccg gtcgagcacc
     2701 agcgtgatga cataaatcgt tgcgacggcc gtccacatgg aaccggcgaa aatatagacg
     2761 ccatacatcg tggaatacca ttcgtgctcc agcgccttga cccaaaggat ggcagccatc
     2821 gtgagcgaaa ggccgaacag gaacaccccg aacgccgaat agcgccgcat catgcgcgtg
     2881 cattcgaccg aaccggtttc gtcctgcttc aaagaccaac cgcgcaattg cgtcgaaagc
     2941 caccaccaga ttcccacgca gataagcgaa gtgatcacga aacccggctg cgtgaacaac
     3001 ggtagttttg ccttggtcgc caaatccgga tggccggcct ggatttcacg cacccattga
     3061 tataatcttg cgcgaaatag aaacaatgga atccatccca gccatgccca gcgcaaagtg
     3121 taagccattt gttccatgat gcgccggatt gtcacagacc aacccgcgtc gaaaagatga
     3181 tggagaatcg taaatccaag gccacccagc gcgatggtca ggaagaacat gaacgcgagc
     3241 agccacgaaa aggcgaattg cgtcaccgcg tcgtgatcca ccaaaaatcc gatgcccaca
     3301 gccgccgcgc cgccgatcat cagccacatc ggcaaggaac gccatctgga caagtccagc
     3361 ggctcggcgg aacttggaga gttggtttgc gaactcataa attatttctt caacgtcgaa
     3421 cgcatctgtt ccggcacgtc gtccacggtc cccaactggc tcaattgcag cgcgcgcaga
     3481 taagcgatga tggcccagcg gtcctcgacc gtcacgttcg ccccgtaagg gcccatcaaa
     3541 ccttttccat tcgagatggt gttgaaaatc tcgccatcct tcatttccac gtaaagtttg
     3601 tcgtgcagat ttttcggcgt ggggggcatc gcgccgactt gcactgttat gcccttgcca
     3661 tcgccttgcg cgccgtgaca gggggaacaa ttgatgttga accgctgttc gccgcgatca
     3721 agcagcttgg cggtgacagt cacaggaatg gtttcgatga aatttgtcgt gcccgggatg
     3781 cggccggtat tttcgggagt gtcggcgtaa taattatctt ccctggcaat ggtgcccacg
     3841 accggcaatt gcgagcttct gccgttctca aaaaatgcgt tcggcacctg cggacgcagc
     3901 ttgggctggc ggttcatgtc cggaaatatt tcaatcggtg gccggcgaga aatggtgccg
     3961 cgcttgccgg cgatcagcat caccgtgacc gtgcacgccg cgaagaagag gagaatatag
     4021 cgcattattc ctccaccagt tcgatgtgca ggctgccgag cgattcaagc agattgcggg
     4081 tttcagtttc ggaatatttc ggatcgcttg tctcaatcac aagataaaaa cggtcgtgac
     4141 tcgcataggc gaagcgccga tgcttgagca acggatgatg caggcgcggg agccggttca
     4201 ggaacaacat gccaagcagc gcgccgaagg agccgaacaa aatcgtcaac tcgtacgaag
     4261 gcggaaacgc gctgtaaggg ctgaacatcg gcttgccgcc gacaacgatg cggtaatcat
     4321 acgcgttcat ccaccaaatc atgatcatcc cggttgtgta accggtcacg ccgcccaaaa
     4381 atgaaaacca gcccacgcgc gaatttttaa ggcccattgc cttgtccatc ccgtgcacag
     4441 gaaatggcgt aaagacgtcc catttgcgat agcccttgtc gcggacattt tccgccgcgt
     4501 gcatcgtgtc agccgggttg aggaattcgg cgatgatgcc gtaggttttt ggagtgtcag
     4561 ccatcagtga tgccctcctt tcgcgccgcc gagcggatta tgcgggtcgg cctgcggtgt
     4621 cacgcccttg acctccgcga tggcaatcag cgggacaaat cggaggaaca gcaggaacat
     4681 cgtgaagaac aaaccgaatg ttccgagata ttgcatgatg tccacccacg ttggccggaa
     4741 ataagcccag tttgatggaa taaattcgcg gtgcagcggc atgatgatga tgacaaaccg
     4801 ctcg
//
LOCUS       DASZMM010000328         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3984890, whole genome shotgun sequence.
ACCESSION   DASZMM010000328 DASZMM010000000
VERSION     DASZMM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3984890"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2069)
                     /locus_tag="VG754_07950"
     CDS             complement(<1..>2069)
                     /locus_tag="VG754_07950"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWF19184.1"
                     /translation="SFDRTVYYIDAPNTGAKTAIDILCDIMQHATLPADEMAKEKQVI
                     LREMDMNQDEPGRRSGRRLFETAYTRSPYRYTIIGYPDIYNELERLDIEEYYRARYAP
                     NNVFYVVVGDIKADDVVAQIRDLYSKTKSRAIPPEVLPEEPRQIAPREAIEEAPIEMG
                     HAHISWHIPELRHPDVPILDVLAVVLGNGRSSRLYQQVREKKGLVNSIDAWTYSPGNP
                     GLFGMSILVDADKFVAARDAALEEVERLRNELVSPEEIAKAVKLFVSATLSARKTMQG
                     QAQDLGGSWIAANDLNFSQRYLAAVKRVTPQDLQRVAREYLPANNRTIYALLPNGTAP
                     KPTISGIESKDSPIQKFELPNGLRLLVKENHRLPFVQFRVAFKGGVLAETPETNGLTQ
                     LTGKLLLKGTTSRSAEEIARTIESVGGSLDYFGGNNSFGVSAEVMSDDFATGLELVSD
                     VLLNPAFPSPALERERQIQLADIKAQRDQLLKSASVAMRRAMFGERGYGLDGLGTERS
                     VQKMTTDALREFHQRLMVPNNCVLAIYGDVKTEAVKAAVEKGFGKWRSGAQALAELPA
                     IAPLAEVKRVKEIRDKKQAVLVIGFPGTTVHSADRYALELLQEACSDLGSRLFMRIRE
                     NLGLAYYVGAQNFPGLAPGYFSFYVGTAPEKVDLVEKELLVEAELLRSEGLSAEELKR
                     AKAKIIG"
BASE COUNT          415 a          605 c          579 g          470 t
ORIGIN      
        1 tggccgatta tttttgcctt ggcgcgcttg agttcttcag cgctcaagcc ttcgcttcgc
       61 agcagttccg cttcaacgag caattctttc tcaaccaaat ccactttttc cggcgcggtg
      121 ccgacgtaga acgaaaaata acccggcgca agtcccggaa aattctgcgc gccgacgtaa
      181 tatgcgaggc ccaagttctc gcggatgcgc atgaagaggc gcgagccgag gtcgctgcag
      241 gcttcctgca acagctccag cgcgtaacgg tcggcgctat gcaccgtcgt gccgggaaaa
      301 ccgatgacca ggacagcttg ttttttgtct cggatttcct tcacgcgctt cacttcggca
      361 agcggcgcaa tcgctggcaa ttccgccagt gcttgcgcgc ccgaacgcca tttgccgaaa
      421 cccttctcaa ccgccgcttt caccgcttcc gtttttacgt ccccatagat tgcgagcacg
      481 caattgttcg gaaccatcag cctttgatga aattcccgca acgcgtcggt ggtcattttt
      541 tggacgctgc gttcggtgcc aagcccgtca agcccgtaac cgcgctcgcc gaacatggcc
      601 cggcgcatcg cgacgctcgc gctcttgagc aactggtcgc gttgcgcctt gatgtcggca
      661 agctggattt gccgttcgcg ttccagagcg ggcgagggaa acgccgggtt cagcaaaaca
      721 tcggacacaa gttccaagcc ggtcgcaaaa tcgtcgctca tcacctcggc gctcacgcca
      781 aaactgttgt tgccgccgaa ataatccaaa ctgccgccga cggattcaat ggtccgcgcg
      841 atttcctcgg ccgaacgact cgtggtgccc ttgagcagca gcttgccggt gagttgcgtc
      901 aagccgttgg tttccggcgt ttcggccagc acgccgcctt tgaacgcgac gcggaattgc
      961 acgaacggca ggcgatgatt ttccttaacc agcaggcgca agccgttggg caactcaaat
     1021 ttttgtatgg gcgaatcttt ggattcgatg ccgctgatgg tgggcttggg cgcggtgcca
     1081 ttgggcagca acgcataaat ggtccgattg ttggcaggga gatattcgcg cgcgacacgt
     1141 tgcaaatcct ggggtgtgac gcgtttgacc gcggcgaggt aacgctggga aaaattcaaa
     1201 tcatttgccg cgatccaact gccacccaaa tcctgcgcct gtccttgcat cgtcttgcgc
     1261 gccgacagcg tggccgaaac gaacaatttc actgccttgg cgatttcttc gggcgaaacc
     1321 agttcgttcc tcaatcgctc gacttcctca agcgccgcat cgcgcgccgc tacgaatttg
     1381 tccgcgtcca cgaggatgct catgccgaac aagcccggat tgccgggact gtaagtccaa
     1441 gcatcaatgg aattgacgag cccttttttc tcgcgcactt gctgataaag tcgcgaactg
     1501 cgaccgttcc caagcaccac ggcgagcacg tccagaatgg gaacgtcggg atggcgcaat
     1561 tccgggatgt gccaactgat atgcgcatgg cccatttcaa tgggagcttc ctcgatggct
     1621 tcgcgcgggg caatctggcg cggttcttcc ggcaaaacct cgggcggaat ggctcgcgat
     1681 ttggtcttgg aatacaagtc gcggatttgc gcaacaacgt cgtccgcctt tatatctccg
     1741 acaacgacat agaaaacatt gttcggcgcg taacgcgcgc ggtaatactc ttcgatgtcc
     1801 aaacgctcca gttcgttgta gatgtccggg taaccgatga tggtatagcg atacgggctg
     1861 cgagtgtagg cggtttcaaa caagcggcgt cccgaacggc gtccgggttc atcctggttc
     1921 atgtccatct cgcggaggat gacctgtttt tccttcgcca tttcatcggc gggcagcgtg
     1981 gcgtgctgca tgatgtcgca caaaatatca atggctgttt tcgcgccggt attgggcgcg
     2041 tcaatgtaat aaaccgtgcg gtcgaagga
//
LOCUS       DASZMM010000329         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4266793, whole genome shotgun sequence.
ACCESSION   DASZMM010000329 DASZMM010000000
VERSION     DASZMM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4266793"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VG754_07955"
     CDS             complement(<1..437)
                     /locus_tag="VG754_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWF19185.1"
                     /translation="METDPFLQAAIDEAKKGLAAGGIPIGSVLVCEGKIIGRGHNQRV
                     QHGSVIHHAEMNCLENAGRLKASVYQKCALYSTLSPCPMCSGAALLYKIPHIIIGENV
                     TFKGPEEYVRSQGVKVEVLQNPECIKLMRDFIAARPELWNEDIG"
     gene            complement(445..>1540)
                     /gene="leuB"
                     /locus_tag="VG754_07960"
     CDS             complement(445..>1540)
                     /gene="leuB"
                     /locus_tag="VG754_07960"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWF19186.1"
                     /translation="AGDGIGPEVMREAIKVLRVAEKKFSFTLKVTEAPVGWTGIDAAG
                     KALPDATLKLCKDSDCILFGSVGLPDRDPAIPKEERPERAALLRLRKEFGLFANLRPV
                     KLPKELSHSCPLSKERQGDGIDILVVRELTGGMYFGQPKKTEEISAGIGKGTIFRAID
                     TMVYTTPEIERIAHVAFRTARSRRKKLTSIDKANVLENGVLWRDVVTTVGKQYLDVAL
                     EHMFVDNGAMQLVLKPTQFDVLLCENMFGDILSDEAAALAGSLGMLPSASLGAQSGDR
                     VFGFYEPAGGTAPDIAGKNLANPIAQILSAALMLRHSFGLNDAANAIEKAVAQAIASG
                     NRTGDIFSATEPNARKVGTTQMGDAIAAAI"
BASE COUNT          304 a          458 c          442 g          336 t
ORIGIN      
        1 ccgatgtctt cgttccaaag ctccggacgg gcggcgataa aatcgcgcat gagcttgatg
       61 cactcgggat tttgcagcac ttccactttc acaccttgcg aacggacgta ttcttccggc
      121 cctttgaaag tgacgttctc gccgatgatg atgtggggaa ttttatagag cagcgccgcg
      181 ccgctacaca tggggcaggg tgaaagcgtc gagtaaagcg cgcatttctg ataaaccgaa
      241 gccttgagac gcccggcgtt ttcgaggcaa ttcatctcgg cgtgatggat cacgctgccg
      301 tgttgcacgc gctggttgtg gccgcgcccg atgatttttc cttcgcacac cagcaccgac
      361 ccgatgggaa tgccgccggc agcaagccct tttttcgctt cgtcaatcgc ggcttgaagg
      421 aatggatcgg tttccataac gcattcaaat cgccgccgcg atcgcatcac ccatttgagt
      481 ggtgccgact ttgcgggcgt tgggttcagt ggcgctgaaa atatcgccgg tgcggttgcc
      541 ggaagcgatg gcttgggcga ccgctttttc aatggcattc gcggcgtcgt tcaggccgaa
      601 actgtggcgc aacatgagcg cggctgagag gatttgcgcg atgggattgg cgaggttttt
      661 cccggcgatg tccggcgcgg tgccgcctgc cggttcgtaa aagccaaaaa cgcggtcgcc
      721 gctctgcgcg ccaaggctgg cgctgggcaa catgccaagc gaaccggcaa gcgcggcggc
      781 ttcgtcgctt aagatgtcgc cgaacatgtt ctcgcaaagc aacacgtcaa actgcgtcgg
      841 tttgagcacg agttgcatcg cgccgttgtc cacgaacatg tgttcaagcg ccacgtccag
      901 atattgtttc ccgacagtgg tgacgacatc gcgccagagc acgccgtttt caagcacgtt
      961 ggccttgtca atgctcgtca attttttgcg gcgcgaccgc gcagtgcgga aggcgacatg
     1021 cgcgatacgc tcgatttccg gggtggtata gaccatcgtg tcaatcgcgc gaaaaatcgt
     1081 ccctttgccg atgcccgcgg aaatctcctc tgttttcttc ggctggccga aatacatccc
     1141 gccagtcagt tcgcgcacga ccaaaatgtc aatgccgtca ccctggcgct ccttgctcaa
     1201 cgggcacgag tgcgacaatt ccttcggcag cttgactggc cgcaaattgg cgaacagtcc
     1261 gaattccttg cgcaaccgca gcaacgccgc gcgttcggga cgttcttcct tgggaatcgc
     1321 cgggtcgcgg tccggcaatc caaccgagcc gaacaaaatg caatccgaat ccttgcacaa
     1381 ttttagcgtc gcatccggca acgccttccc ggcggcgtca atcccggtcc aaccgactgg
     1441 cgcttcggtg accttgagcg tgaacgaaaa cttcttttcg gcaacgcgca agaccttgat
     1501 ggcctcgcgc atgacttcag ggccgatacc atcgccagca
//
LOCUS       DASZMM010000330         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2334365, whole genome shotgun sequence.
ACCESSION   DASZMM010000330 DASZMM010000000
VERSION     DASZMM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334365"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VG754_07965"
     CDS             <1..282
                     /locus_tag="VG754_07965"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HWF19187.1"
                     /translation="ATRPTPAEDWVTDLQTGKPAARGSWNYNGSRVVDGIYMAQQQGS
                     IVAMVDDVDAMVNNPRKGHENDQVWEVRSNAVPKLNTKVMVRFRLEAGR"
     gene            450..1664
                     /locus_tag="VG754_07970"
     CDS             450..1664
                     /locus_tag="VG754_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF19188.1"
                     /translation="MADRSAALVQIDRLGLFPIAVDMPKGAAAAALADKAGAKQANLD
                     FLPPALRNVLKRKRKPKMQELATFTQQLANLLKAGMPLTTALNSMTYLKSKGIPSDVA
                     KQLKHDVMEGKGLSDAMAKQPVIFSNLYINMVKAGEQSGALEDVLRRMADHFTRFAEV
                     QSKFKSALIYPSFVLMVGIGIGIFFMTVMLPNFLKLFDGFKVPLPLATRMLIGVSNFF
                     QHWWWLLLAVVSTIVMMAYRFSTTEHGKRQIDQWKISVPVIGNVMKLNLFGQFARTLA
                     TLLHNGVPVLTALEITEQIIPNRIVKEAIAQTRREVTDGKTIAQPLGRSKVFPQLMVD
                     LVKIGEETGNVPGSLANVADTYENELNIALRVMTNMIEPVIIVFMAMGVGFLLVAVLS
                     AMFSITSSISAR"
     gene            1709..2173
                     /locus_tag="VG754_07975"
     CDS             1709..2173
                     /locus_tag="VG754_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF19189.1"
                     /translation="MQFRTNKFHKGFTLIEIMIVVSIMGLILAMGMPSFVKTLHKEGM
                     NKAVKDIREACLAARADAILHQKTADLIFHPADRTISAPGFKEATLPNNVEIQILGVN
                     FVQYERADEAKIHFFPNGTSDEFTIVVQDDQFQTRQISLDVMTALPEMEVIR"
     gene            2170..2598
                     /locus_tag="VG754_07980"
     CDS             2170..2598
                     /locus_tag="VG754_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19190.1"
                     /translation="MKVQIQKLNAKGTRAFTLVEVVVAVAVFFMAMFAILGVMSQCIR
                     AVSSLQKDSPTAGMVAAMAMANDKWEEGTDSGDFGDFYPDYTWETQTNRYEGITPGKD
                     GNQPPFFELDITVKHKRDIVSALSVLHYSPNSPMKSGMSH"
     gene            2598..3272
                     /locus_tag="VG754_07985"
     CDS             2598..3272
                     /locus_tag="VG754_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19191.1"
                     /translation="MRIFNRKSNQDGGKCSLLAFTLLEILVAISLFTLVLISIFQCWK
                     LIANGSRIGLEAAGSAQRARTGILTIQEALQTAQMSLGSLQYYTFEADTSGKFAALAF
                     TARLPDSFPGSSLFGDSSTRRITFQVERDKDNKNNLVMTQWPLLVAITKDATPYPIVL
                     ARDVSVFNVEFWSDKEKDWVTEFLETNQIPKMMRVALGVGYSAHDFNRPQQLVTRVVA
                     PASSPH"
     gene            3269..>3470
                     /locus_tag="VG754_07990"
     CDS             3269..>3470
                     /locus_tag="VG754_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19192.1"
                     /translation="MRTKLTSSKRAEGGIALIMVMIIILTLSTIVGGFAYAMKVEMRL
                     ARNSDYDNDMVWLGRSGVELARY"
BASE COUNT          778 a          782 c         1106 g          804 t
ORIGIN      
        1 gcgacgcgcc ccactccggc ggaggattgg gtgacggatt tgcagacggg caagccggcg
       61 gcgcgcgggt cgtggaatta taacgggtcg cgcgtggtgg atggtattta tatggcgcag
      121 cagcaggggt cgattgtcgc aatggtggat gatgtggatg cgatggtgaa taatccgcgc
      181 aagggtcatg agaatgatca ggtgtgggag gtcaggagca atgcggtgcc gaaattgaac
      241 acgaaggtga tggtgaggtt cagactagaa gctggaagat agaagataga ggcttgaaac
      301 ttttgggcta aagcgggttt aatatggtag agcaggattt tttctttggg atgacggggt
      361 tttttgagtt tttagaaaca taacaaatgc cgcaattcgc ttataaagca cgaagacgct
      421 ccggtgaggt ggtcactggt gtcctggaag tggccgaccg cagtgcggcg ctggtccaga
      481 tcgaccgcct ggggctgttc ccgatcgctg tggatatgcc caagggcgcg gcggcggctg
      541 cgttggccga taaggcaggc gcgaagcagg cgaatttgga ttttctgccg ccggcgttgc
      601 gcaatgtcct gaaacgcaaa cgcaagccga agatgcagga gttggcgacc ttcacccagc
      661 agctcgccaa cctgctcaag gccggcatgc cgctgacgac ggcactgaac agcatgacgt
      721 atttgaagag caagggcatt ccttcggacg tcgccaagca gctcaagcat gacgtcatgg
      781 agggtaaagg gctttcggac gcgatggcga agcagccggt gattttctcg aatctctaca
      841 tcaacatggt gaaggcgggc gagcaatccg gcgcgctgga agatgtgttg cgccggatgg
      901 cggaccattt cacgcggttt gcggaggtgc agtcgaagtt taaatcggcg ctgatttatc
      961 cgtcgttcgt tttgatggtc ggtatcggca tcggcatttt cttcatgacg gtgatgttgc
     1021 cgaacttttt gaaattgttc gatggattca aggtgccgct gccgctggct acgcgcatgc
     1081 tcattggtgt gagcaacttt ttccaacatt ggtggtggct gctcctggcg gtggtttcga
     1141 ctatcgtgat gatggcttac cggttcagca cgactgaaca tggcaaaagg caaatcgatc
     1201 agtggaagat ttcggtgccg gtgatcggga acgtgatgaa gttgaatttg ttcggccaat
     1261 ttgcgcgaac gctggcgacg ttgttgcaca acggcgtgcc ggttttgacg gcgctggaga
     1321 ttacggagca gattattccc aaccgcatcg tgaaggaagc aatcgcgcaa acgcggcgcg
     1381 aagtcacgga cggtaaaaca atcgcgcaac cgctggggcg cagcaaagtg tttccgcaat
     1441 tgatggtcga tctggtgaag atcggcgagg aaacgggcaa tgtgcccggt tcgctggcca
     1501 acgtggcgga cacttacgaa aacgaattga acatcgcgtt gcgcgtgatg acgaacatga
     1561 ttgagccggt cattattgtg ttcatggcga tgggtgtcgg cttcttgctg gtcgccgtgt
     1621 tgtccgcgat gttctcaatc acgtcgagta tttcggcgag gtagcggcat gtgggctcgc
     1681 cgctcgcatt cgcagctgga aaaaattgat gcagtttcgc acaaacaaat ttcacaaggg
     1741 atttacgctg atcgaaatca tgatcgtggt ttcgatcatg ggattgattt tggcgatggg
     1801 catgccgtcg tttgtgaaaa cgttgcacaa ggagggcatg aataaggccg tcaaggacat
     1861 ccgggaggcg tgcctggcgg cgcgagcgga tgcgattctg caccagaaga cggctgattt
     1921 gatttttcat ccggcggacc ggacaatttc ggcgcccggc ttcaaggaag caactttacc
     1981 caacaatgtg gagatccaga ttctcggtgt gaatttcgtg caatacgaac gggcggacga
     2041 ggcgaagatc cattttttcc cgaacggcac gagtgacgag ttcacgattg tggttcaaga
     2101 cgatcagttc cagacgcgcc agatttcact cgatgtgatg acggcgttgc cggagatgga
     2161 ggtcattcga tgaaggtgca gattcaaaag ttaaatgcta aaggaacgcg ggcgttcacg
     2221 ttggttgaag tggttgtcgc ggtcgcggtg tttttcatgg cgatgtttgc gatcctcggc
     2281 gtgatgagcc aatgcatccg cgcggtcagc agtttgcaga aagattcgcc gacggctggg
     2341 atggtcgcgg cgatggcgat ggccaatgac aaatgggaag aagggacgga ctctggcgat
     2401 ttcggcgatt tttatccaga ttatacgtgg gaaacgcaga cgaatcgcta cgaaggcatc
     2461 acgccgggaa aggacggaaa tcagccgccg ttttttgaat tggatatcac ggtcaaacac
     2521 aagcgagata tcgtctcggc attgagcgtt ttgcattact cgccgaattc gccgatgaaa
     2581 agcgggatga gccattgatg agaattttta atcgaaagtc gaatcaggac ggtggaaagt
     2641 gttcgcttct ggcgtttacg ctgctggaaa tccttgtcgc tatttcgctg ttcacgctcg
     2701 tgttaatcag cattttccaa tgttggaagt tgatcgcgaa cgggtccagg atcggtttgg
     2761 aagccgccgg cagcgcacag cgggcgcgca cgggaatttt aacgatccag gaggcgttgc
     2821 agaccgcgca aatgtcgctg ggcagtttgc agtattacac gtttgaagcg gacacgagcg
     2881 ggaagttcgc agcgctcgcg tttaccgcgc gtttgccgga cagttttccg ggctcgtcgt
     2941 tgttcggcga ttcgagcacg cgacgaatca ctttccaggt ggagcgcgac aaggacaaca
     3001 aaaataatct ggtcatgacg caatggccgt tgctggtggc gatcacgaag gacgcgacgc
     3061 cctaccctat cgtgctggcg cgcgatgtga gcgtgttcaa tgtcgaattt tggtcggaca
     3121 aggagaagga ttgggtcacg gaatttctgg agacaaacca gattccgaaa atgatgcgcg
     3181 tcgctctcgg cgtcggttat tcggcgcatg attttaaccg gccacaacaa ttggtcacgc
     3241 gcgttgttgc gccagcgtca tcaccccatt gagaactaaa cttacatcaa gcaaacgtgc
     3301 ggaaggcggc atcgcgctga tcatggtgat gattatcatc ctgacgctgt cgacgattgt
     3361 cggcggcttt gcttacgcga tgaaggtgga gatgcggctc gcgcgcaatt ctgattacga
     3421 caacgacatg gtttggctgg ggcggtccgg tgtggaactg gctcgttacg
//
LOCUS       DASZMM010000331         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4806910, whole genome shotgun sequence.
ACCESSION   DASZMM010000331 DASZMM010000000
VERSION     DASZMM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4806910"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1006
                     /locus_tag="VG754_07995"
     CDS             275..1006
                     /locus_tag="VG754_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWF19193.1"
                     /translation="MKNLKRWAIWIGVFCAIGAQCSAAEWLTDYDVALGKARAEHKTI
                     LLDFTGSDWCGWCKKLKSEVFDQPEFDAFANANLVLVEVDFPRHWVLSRAQQDANNSL
                     AQKYGIRGYPTIILLDASGNKIAETGYRDGGPKPYIAHLEKITAMKHSSSTPDAAPPR
                     PAPEFVPIPAAKPIQYGELALKAISGSAGRRMALINNETFLVGETAKVKVKESKVEVT
                     CKEIRADSVLLTVDGKIIELKLPHQ"
     gene            complement(1130..1819)
                     /locus_tag="VG754_08000"
     CDS             complement(1130..1819)
                     /locus_tag="VG754_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19194.1"
                     /translation="MNGTNVTHSELPEERTERSLRTVAGGGSLVEAIGGIAAIVLAIF
                     GLAGGMANDMMAIATIVLGASLILEGGAIAASYRRALFALEGETAGSAELGGAATVEF
                     LAGFTGIVMGILALLGVGTLNYFISVSAIVFGASLALSSSAVARLSAFWSSNWYLNES
                     SRSIAQTSCETRAGGQMLVGLAAIVLGILALLGINSVVLNLVALLTLGVSVLLSGTAF
                     GARTMMHTKRA"
     gene            complement(2016..3233)
                     /locus_tag="VG754_08005"
     CDS             complement(2016..3233)
                     /locus_tag="VG754_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWF19195.1"
                     /translation="MDFHSLIAMAKANGASDLHLEPGLPAAVRIRGSLKASGAPLPGK
                     ALLEAAQELIGNEGWNEFLRRRSYDLSRSIQGVRCRINVMQTSRGVGMAVRLLMGFQA
                     TVEKLNLHPDLRKLIGHTTGLIIISGATGSGKSSTLAALLQEINLTETRHIVTIESPI
                     EYVFKPHHAYIRQREVGRDTPSFEQALIDAMREDPDVLMVGEMRDPETMRLTLNAAET
                     GHLVLATAHSSTAAEALQRVVAAFPSEIQNSVCAQLADCLVAVVCQRLQYRTDLKIQV
                     PECEVLMPTHSVKNFIRRGEFFKMMSSMETGASEGMWTYARYRSWLETKKNWHLPGEK
                     IEDEPAPEMSSIPEVQFAPPPISKSPAATPAPKLTPTPLSAGAPKSKDDLLEIEPEEG
                     GLEAIISKLKSKP"
     gene            complement(3215..>3465)
                     /locus_tag="VG754_08010"
     CDS             complement(3215..>3465)
                     /locus_tag="VG754_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19196.1"
                     /translation="SSTYFPPSSRRNFRLIPLVSTPFRLIPDKKINFFEGKLIVLALL
                     RHAFAIRYPRQGKDFFLKTGNDFPPMVIIWKRLWIFIR"
BASE COUNT          801 a          956 c          910 g          798 t
ORIGIN      
        1 actcagcgca ttggcaaatg acggtttgtt gcgcgcccgt ttgcgcgccg ccgggttgaa
       61 acgcgcgcaa caatttaact ggcaccgcac ggctgctcag acgctggaag tttatcgtgc
      121 ggtttcttcg cggtgaaaac attatccagc cgtctgcgtt cttcaaagct ggcataaata
      181 tcgcttgaat ctcccagcct ttgcggcgtg atgcctttgg ttttttcatc tgtcctgttt
      241 tgggacgaaa ccaacgcacc gcaggtctaa aaaaatgaaa aatctcaagc gttgggcgat
      301 ttggatcggt gtgttctgtg ccatcggtgc gcaatgcagc gcggcggaat ggttgaccga
      361 ttatgatgtc gcgctcggaa aagcgcgcgc tgaacacaaa accatcctgc tcgatttcac
      421 gggctccgat tggtgcggct ggtgcaagaa gttgaagagc gaagttttcg accagcccga
      481 gtttgacgct ttcgcgaacg cgaatcttgt cctggtggaa gtggattttc cgcgtcattg
      541 ggtgttaagc cgcgcgcagc aggacgccaa caacagcctc gcgcaaaaat atggcatacg
      601 cggttatccc acgatcattt tgctggatgc atcgggtaac aaaatcgccg agacgggcta
      661 tagagatggc ggaccaaagc catacatcgc gcatcttgaa aaaatcaccg ccatgaaaca
      721 ttcgagttcc accccggacg ctgctccgcc ccgccccgcg ccggaatttg ttccgatccc
      781 cgcagccaag ccgattcaat atggcgaact cgcgttgaag gccatttcgg gttctgccgg
      841 ccgccgcatg gcattgatca acaatgaaac atttctcgtg ggcgaaaccg ccaaggtgaa
      901 ggtgaaggaa agcaaggtcg aagtgacgtg caaggaaatc cgcgcggatt cggtgttgct
      961 caccgtcgat ggaaaaatca tcgaactgaa gcttccgcat cagtgacgaa aacacgtttt
     1021 ctctgtgcga aaaaacgtga gaggcagagg ccgccgaatt tcagcagccc tttcaacctc
     1081 tcacgcagac agtcattcat tcaggttcca gttcctgatt taatcgcatt caagcgcgct
     1141 tggtgtgcat catcgtgcgc gcgccaaaag ccgttccgct caagaggacc gaaacgccca
     1201 gcgtcaacaa cgccacgaga ttgagcacga cgctattgat tcccagcaag gcaaggatgc
     1261 ccaggacaat cgccgccaag ccgaccaaca tctggccgcc agcgcgggtt tcgcacgagg
     1321 tctgcgctat ggaacgcgaa ctttcattca aataccaatt tgaactccag aacgcgctca
     1381 atctcgccac cgccgaactg ctcaacgcca gcgaggcgcc aaacacaatt gccgatacag
     1441 agatgaagta atttagagtg ccaactccca acaacgcgag gatgcccatc actattcccg
     1501 tgaagcccgc caggaattcc accgtcgccg ctccgcccag ttccgctgaa ccggctgttt
     1561 ctccttccaa ggcaaaaagc gccctgcgat agctcgccgc aattgcgccg ccttccaata
     1621 tcaacgaagc gcccagcacg atggttgcga tggccatcat gtcgtttgcc attcctcctg
     1681 ccagtccaaa aattgcgagc acaatcgccg caatccctcc tatagcctcc actagtgagc
     1741 cgccgcctgc gacggttcgt agcgaacgtt cggttcgttc ttcaggtagt tcgctgtggg
     1801 taacattcgt gccattcata aaagtccttc ctttcaaagt tgattgtttc tctgtttggt
     1861 ttcagttcaa agggccactt ctaccctcta ttaatttaga caggatggtg atggcgtttt
     1921 atcaaatatc gcggaggatt tttaggactt taaaaccttt ataattaaag acttatggca
     1981 taaaaaatgt gaccaatccc tcttcatggc ttcctttatg gttttgattt caatttcgaa
     2041 atgattgctt ccaagccgcc ttcttctggc tcgatttcca gcaggtcatc cttcgacttg
     2101 ggagcgcctg ccgataaggg tgttggtgtc agtttcgggg caggtgttgc cgcgggggat
     2161 ttcgaaattg gcggcggagc aaattgcact tctgggatgg atgacatttc cggggcgggt
     2221 tcatcttcga ttttctcgcc aggcaaatgc cagttctttt tcgtttccag ccaggagcga
     2281 tagcgcgcat aggtccacat cccttcgctc gcgccggttt ccatcgagga catcattttg
     2341 aaaaattcgc cgcgccggat gaaattcttg accgagtggg tcggcatcaa cacttcgcac
     2401 tcgggcacct ggattttcaa atcggtgcga tattgcaatc gctggcagac cacggccaca
     2461 aggcaatcgg ccaattgcgc gcaaacgctg ttttggatct cggacggaaa tgccgcaacg
     2521 acgcgctgca atgcttccgc cgcagttgat gaatgcgcag tcgccaagac gagatggccc
     2581 gtttccgccg cgttcaaggt caatcgcatc gtttccggat cgcgcatttc gccgaccatc
     2641 aacacgtccg gatcttcccg catcgcatca atcagcgcct gctcgaatga cggcgtgtcg
     2701 cggcccactt cgcgctgccg gatgtacgcg tgatgcggct tgaacacata ttcaatcggg
     2761 ctttcgatgg tgacgatgtg ccgtgtctcg gtgagattga tttcctggag caacgccgcg
     2821 agggttgaag attttcccga acccgtcgcg ccgctgatga tgatcaaccc ggtcgtgtgg
     2881 ccgatcaact tgcgcaggtc cggatgcagg ttcaattttt caaccgtggc ctggaatccc
     2941 atcaacaaac gcaccgccat gcccacgccg cgcgaagttt gcatcacgtt gatgcggcag
     3001 cggacgcctt ggatgctgcg cgacaaatcg tacgaacgcc gtctcaggaa ttcgttccaa
     3061 ccttcgttgc cgatcaattc ctgggcggct tcgagcagcg ccttgccggg aaggggcgcg
     3121 ccgctggctt tgagcgagcc tcggatgcgg acggcggccg gcaagcccgg ttccagatgc
     3181 aagtcactgg ccccattggc cttggccatg gcaatcaacg aatgaaaatc cataaacgct
     3241 tccatattat aaccattgga gggaaatcgt tgccagtttt aagaaaaaag tccttgcctt
     3301 gacgaggata gcggatggca aaggcgtggc gcaggagcgc taatacgatg agtttacctt
     3361 caaaaaaatt aattttttta tcaggaatta agcggaacgg ggtggaaaca agcggaatta
     3421 agcggaaatt tctccgcgac gatggcggaa aatacgtgga tgaaa
//
LOCUS       DASZMM010000332         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_125547, whole genome shotgun sequence.
ACCESSION   DASZMM010000332 DASZMM010000000
VERSION     DASZMM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125547"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="hflX"
                     /locus_tag="VG754_08015"
     CDS             complement(<1..904)
                     /gene="hflX"
                     /locus_tag="VG754_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWF19197.1"
                     /translation="MKALISTATKRTERVFLVGVELKSRSVLDTRESLDELSELASTA
                     GGDVIGDGTQKMEAPVAGTFIGSGKADEFAQHCRRHDVDTVIFDDELSPAQSRNLERV
                     FDCKVLDRTSLILDIFAQRARTREGKLQIELAQLQHLLPRLTRFWGHLSRQKGGIGMR
                     GDGETQLETDRRRVQDRIAKIARELEVVRRQRSTQRQGRQRNQWPLASIVGYTNAGKS
                     TLLNALTGAEVLAENKLFATLDPTTRRLRLPTNQNVLLTDTVGFIRKLPHGLVEAFKA
                     TLEEVVQADLLLHVVDVSHPQAAEQ"
     gene            complement(1150..1902)
                     /locus_tag="VG754_08020"
     CDS             complement(1150..1902)
                     /locus_tag="VG754_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWF19198.1"
                     /translation="MKVIILAAGYATRLYPLTLTQPKPLLPVAGKPMIDHVLDNLAGI
                     KGIDRVYVVTNAKFAGHFQKWAEAYRVEKTRLDFTVVNDGSTDDKNKLGAIGDLHLVL
                     TREKVDDDVIVVAGDNLFSERLDGFAEAARRRNAPMEAVYDVGSLEEVKKYNSISVDA
                     EGRITFFEEKPKNPTSTLTGIALYYYPKAVLPLIHQYIAEGNNPDQPGRLVQWLYPRV
                     PVYTWKVPGLWFDIGSKETLEEANRIFAKSQK"
     gene            complement(2046..3824)
                     /gene="ptsP"
                     /locus_tag="VG754_08025"
     CDS             complement(2046..3824)
                     /gene="ptsP"
                     /locus_tag="VG754_08025"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWF19199.1"
                     /translation="MPEPAHKGEKVFRGIPVSAGVCRGKVLVLGRAHSTIPKHEVRDE
                     HLHEEINRLERALIQTRQQILEVQRKVCDGMGAEEGGIFDAHLLVLEDRTLLDEVMRN
                     INEQKVNAEYAFHIVAEKYATTLAAIEDDYLRERATDMRDVTSRILNNLLGRQEDIDL
                     KKLKEPCVIISYDLSPSTTAQMDRRVVLGFATDIGGKTSHSAIMARSLRIPAVVGLKN
                     LSQELETGQYILLDGFNGIVILNPTDATLFEYGQIIRNQVSLEEKLKDIQGKPAVTMD
                     GHQIMLSANVEHPSDASEVQGNGAEGVGLFRTEYLFINRTTLPTEEEQYQAYRSIAVA
                     LKPHPVVVRTMDLGGDKFLSHLKLPAEMNPFLGWRAIRYSLQEKPVFSTQLRAILRAG
                     AEGNVKMMYPMISGVEELKQANAVVEECKAELRAEKIPFDENLEIGAMIEIPSAAMVA
                     DALARHLKFLSLGTNDLIQYTLAVDRMNERVAHLYEPTHPAIIRLIKYTVDAAHKNNI
                     WVGVCGEMAGDPTLTALLLGLGVDELSAAPPLVPPVKFMIRRLKMNEARTLADFALNC
                     DSSSEILTRCQALAREIAPSLFESKS"
     gene            4133..5185
                     /locus_tag="VG754_08030"
     CDS             4133..5185
                     /locus_tag="VG754_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWF19200.1"
                     /translation="MKTKSARPRSGLLAGGNWIIDQVKLIDVYPQREQLANILSQSQG
                     TGGAPYNVLIDLARMGVSFPLFAAGLVGDDSLGREILADCQRHKIDTRQLTITSKAPT
                     SYTDVMTELSGGHRTFFHARGANALWQGEDINFNKTSARIFHLGYLLLLDALDGQDAV
                     YGTKAARLLAAAQKAGLKTSVDVVSEDSDRFSKIVQPALKHVDYCILNEIEAGKTTGF
                     KIRLPDGRLDTAALRHAAGALLQNGVRELVVIHFPEGGFARTRKGEDVWQSSLKLPAK
                     HVVGTAGAGDAFCAGTLLGLHEGWELSKCLLTGVCAAAASLSDATCTAGVGSLRSCLA
                     LGKKFGLRPRLATSEE"
BASE COUNT         1080 a         1418 c         1453 g         1246 t
ORIGIN      
        1 tctgttccgc ggcctgcggg tggctcacat cgacgacgtg cagcaacaaa tccgcctgca
       61 caacttcttc gagcgtcgcc ttgaacgcct cgaccaagcc atgcggcagt ttgcggatga
      121 aaccgaccgt gtcggtcagc aacacgtttt ggttcgtggg caaacgcagc cggcgcgtgg
      181 tggggtccag cgtcgcgaac aatttgttct cggccaaaac ttccgcgcca gtgagcgcgt
      241 tgagcaacgt ggatttgccg gcgttggtgt agccgacgat cgaggcaagc ggccattggt
      301 tgcgctggcg tccctggcgt tgcgtggaac gctggcggcg gacgacttcc agttcgcggg
      361 caatctttgc gattcggtcc tgcacacggc ggcggtcggt ttccaactgc gtttcaccgt
      421 caccgcgcat gccgatgccg cccttttggc gcgacaagtg gccccaaaac cgcgtcaagc
      481 gcggcagcaa atgctggagt tgcgcgagtt caatctggag cttgccttcg cgcgtgcggg
      541 cgcgttgggc gaaaatatca aggatcagcg acgtgcgatc gagcaccttg caatcgaaaa
      601 cgcgctccaa gttgcggctt tgcgccgggg aaagttcatc gtcgaaaata accgtgtcaa
      661 cgtcatgacg ccggcaatgt tgcgcaaatt cgtccgcctt gccgctcccg atgaaagtgc
      721 ccgcgacggg agcttccatt ttctgtgtgc cgtcgccaat gacgtccccg ccggctgtgg
      781 aggcaagttc agaaagttcg tcgagagatt cgcgcgtgtc gaggacgctg cgggatttga
      841 gttccacgcc gacgaggaag actcgttcgg tgcgttttgt ggcggtcgaa attagtgctt
      901 tcaattagct tcggatgatt ctctctatca ggagcaatat agcatgaaaa aacagggcgc
      961 aaagggaatt tcaggttaca aaattctgaa ttcaaatgaa aaaaggatac cggggaagag
     1021 tttttgacaa gcgatcaaat gatctgggct gttttttaat taatgatgct ccttgacata
     1081 atctcaaaaa tttttttacg gtttttttaa gcttactttt tgaagttagt tttgggaatc
     1141 gtttggtttt tacttttggc tctttgcaaa aatccggttc gcctcttcca gcgtttcctt
     1201 tgagcctatg tcgaaccaaa gtcccgggac tttccacgta tagacgggta cgcgcggata
     1261 aagccactgc acaaggcggc caggctggtc gggattgttc ccttccgcga tgtattggtg
     1321 aatgagcggc aggacggcct tgggataata atagagcgcg atgccggtga gcgtgctggt
     1381 cgggtttttc ggcttttcct caaagaaggt gatgcggcct tcggcatcca cggaaatgga
     1441 attgtacttt ttcacttcct caaggctgcc gacgtcataa accgcctcca tcggcgcgtt
     1501 gcggcgtcgc gcggcctctg cgaagccgtc caatctttcg ctgaacagat tgtcaccggc
     1561 gaccacgatg acgtcgtcat ccactttttc acgggtgagg acaagatgca gatcgccgat
     1621 tgcgccaagt ttatttttat cgtccgttga gccgtcattg acgacggtga aatcgaggcg
     1681 cgttttttcg acgcgatagg cttcggccca tttttggaaa tggccggcga atttggcgtt
     1741 ggtgacgaca tacacgcggt caatgccctt gatgccggca aggttatcga gaacgtgatc
     1801 aatcatcggc ttgccggcga ccggcagcag cggtttgggc tgggtaagcg tcaatggata
     1861 aagccgcgtg gcgtaacctg cggcgagaat gatgaccttc atagtttaat ttagcagaaa
     1921 aatggatttt tcaagcgaca tcgaacggtg aacattgaac gccgaacatc gacttgcgga
     1981 ctgtgtgata ggccccattt ccattctgtg ttcagtgttg gatgttcgat gtttctcaaa
     2041 ccagcttacg acttgctctc gaacaggctg ggtgcgatct cccgcgccaa agcctggcag
     2101 cgggtgagga tttccgaact cgaatcgcaa ttcaaggcaa agtcggccag tgtgcgcgcc
     2161 tcattcatct tgagccggcg gatcatgaac ttgaccggcg gcacaagcgg cggcgcggcg
     2221 ctcaattcat ctacgcccaa accgagcaac aacgccgtca gcgtcgggtc gcccgccatc
     2281 tcgccgcaga caccgaccca gatattgttt ttgtgcgcgg catccacagt gtatttgatg
     2341 agacggatga tggccggatg cgtcggctcg taaagatgcg ccacacgctc gttcatgcgg
     2401 tccaccgcga gtgtgtattg gatcaaatca ttcgtgccga gactgagaaa tttgagatgc
     2461 ctggccagcg cgtcggcgac cattgccgcg gacgggattt caatcatcgc gccaatttcc
     2521 agattttcat cgaacggaat cttttcggcg cgcaactcag ccttgcattc ctcgacgacg
     2581 gcattggctt gcttgagttc ttccaccccg gaaatcatcg gatacatcat cttcacattg
     2641 ccttccgcgc cagcgcggag gatggcccgc aattgggtgc tgaacaccgg cttttcctgc
     2701 aacgaatatc gaatcgcccg ccagcccaaa aacgggttca tctccgccgg caatttcaga
     2761 tgtgacaaaa atttgtcgcc gcccaaatcc atagtgcgga ccaccacggg atgcggcttc
     2821 aatgccacgg cgatgctgcg atatgcttgg tattgctcct cctcggtcgg cagcgtcgtg
     2881 cggttgatga aaagatattc cgtgcggaac aaaccaacgc cttccgcgcc gttgccctgg
     2941 acttcactgg catcgctcgg atgttccacg ttggcggaga gcatgatctg gtgcccgtcc
     3001 attgtgacgg cgggcttgcc ctggatgtcc ttcagttttt cttcgaggct gacctggttg
     3061 cggatgatct ggccatattc gaacagcgtg gcgtcggtcg gattcaggat gacgatgccg
     3121 ttgaaaccat cgagcaggat gtattggccg gtttcaagtt cctggctgag attcttgagg
     3181 ccaaccaccg ccgggatgcg caaagaacgg gccatgatgg cgctgtgcga agtctttccg
     3241 ccaatatcgg tcgcaaagcc aagcacaacc ctccggtcca tctgggcagt agtggaagga
     3301 ctcaggtcgt agctgatgat gacgcacggt tccttgagtt tcttgaggtc aatgtcctcc
     3361 tgacgtccca gcaaattgtt caggatgcgc gaagtgacgt cccgcatgtc ggtcgcgcgt
     3421 tcgcgcagat aatcatcttc aatcgccgcg agcgtggtgg cgtatttttc agcgacaatg
     3481 tggaatgcgt attcggcgtt gaccttttgc tcgttgatgt tgcgcatgac ttcatcgagc
     3541 aacgtgcggt cttcaagcac cagcaaatgg gcgtcaaaaa taccgccttc ctcggcgccc
     3601 atgccatcgc aaaccttgcg ctgcacttcg aggatttgct ggcgggtctg gatgagggcg
     3661 cgttcgaggc ggttgatttc ctcatgcaaa tgttcatcgc ggacttcgtg cttggggatc
     3721 gtgctatgtg cgcggccgag cacaaggact ttgccgcggc aaacccctgc tgaaacggga
     3781 ataccccgaa acactttctc acccttgtgg gctggctctg gcatggacaa tcattacatg
     3841 gaacccgcgg cattgaaaag attttcttta accgcatgct gaacggttcc cgttagattg
     3901 gtgattcgtt acaaggttaa atggttacat ctgcattgcc aataacgccg gttgttcttg
     3961 cggccattta acaattcaac catcaaacat cagcagacgc ccgttcatca gcgcacgcgg
     4021 ttgtgtaaaa atcgcttgcc atcgggccat gtattttctt tcactagtcg cgacctctga
     4081 tatggatagt atgcaccaac gtccttagga tgtcctccaa tttgacttta tcatgaagac
     4141 taaatctgcc aggccccgtt ccggattgct cgccggaggc aactggatca ttgatcaggt
     4201 caaactcatt gatgtctatc cccaacgcga acaactcgcc aacatcctca gccaatctca
     4261 aggcaccggc ggcgcgcctt ataatgtctt gattgatctc gcccggatgg gcgtttcctt
     4321 tccgcttttc gccgccggcc tcgttggcga cgattccctt ggccgcgaaa tccttgccga
     4381 ttgccagcgt cataagattg acacacgcca actgaccatc acaagcaagg cgccgacttc
     4441 ctataccgat gtgatgaccg agttaagcgg cggtcaccgg acattttttc acgcgcgcgg
     4501 cgcgaatgct ctctggcagg gcgaggacat caatttcaac aaaacgtccg cgcgcatttt
     4561 tcacctcggc tatctgttgc tgctggacgc cctggatggt caggacgccg tttacggcac
     4621 caaggctgcg cgtttgctag cggcggcgca gaaagccggg ctcaaaacca gcgtagatgt
     4681 tgtcagcgaa gacagcgatc gtttttctaa aattgtccag cccgcgctca agcacgtgga
     4741 ctattgcatc ctcaatgaaa tcgaggccgg caaaaccacc ggtttcaaaa tccgcctgcc
     4801 cgatgggcgg ctggacacgg ctgcgttgcg tcacgccgct ggcgcattgc tgcaaaatgg
     4861 cgtccgcgaa ctcgtggtca ttcattttcc cgaaggggga ttcgcccgca cgcgcaaagg
     4921 cgaggacgtc tggcaatcgt cgctgaaatt gcccgcgaaa catgtcgtgg gtaccgctgg
     4981 cgcgggcgac gcgttttgcg caggcacatt gcttggcttg catgaaggct gggaactttc
     5041 caaatgcctg ttgaccggcg tttgcgccgc agcggcttcc ttgtcggacg cgacttgcac
     5101 tgcgggagtc ggttcgctgc ggagctgcct tgcccttggc aaaaaattcg gccttcgccc
     5161 gcgtcttgct acatccgaag aataaaagtt gggtctt
//
LOCUS       DASZMM010000333         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4268831, whole genome shotgun sequence.
ACCESSION   DASZMM010000333 DASZMM010000000
VERSION     DASZMM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4268831"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VG754_08035"
     CDS             complement(<1..193)
                     /locus_tag="VG754_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HWF19201.1"
                     /translation="MYSPTLEQFLALAKEGNVIPVTRRLLADFETPLSAYHKIRGHGE
                     SFLFESVEGGEHLGRYSFVG"
     gene            complement(211..735)
                     /locus_tag="VG754_08040"
     CDS             complement(211..735)
                     /locus_tag="VG754_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19202.1"
                     /translation="MDDFVLFGSGIYCVVFALLAFARGMDFLSTWVATPNLVLEGNPL
                     AKKLGWRWGIPVNVVLCFAFAFWPLAAIMISTTSVLVAARNFQHAWLMRSLGEESYRS
                     WHIARIQETRVSLFLYCLLGQTALTGGVGAALIWFSLPQVVPLAIGLGIVSYAIAVAF
                     YTLLSVWRMRRALG"
     gene            complement(736..1128)
                     /gene="hisI"
                     /locus_tag="VG754_08045"
     CDS             complement(736..1128)
                     /gene="hisI"
                     /locus_tag="VG754_08045"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWF19203.1"
                     /translation="MNTIYDNLKFNADGLIPAIIQEQSTGRVLMMAWMNRASLESTIA
                     TGKTHFWSRSRKKFWMKGESSGHTQQVKDIAFDCDGDTLLIQVEQIGAACHEGYKSCF
                     FRSATDGGNSFKNTEPQLAKPDEIYGKK"
     gene            complement(1202..1558)
                     /locus_tag="VG754_08050"
     CDS             complement(1202..1558)
                     /locus_tag="VG754_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HWF19204.1"
                     /translation="MNALLQSRWLPIVLLTCSNIFMTFAWYGHLKYKNHPIWQVIFAS
                     WGIAFFEYLLQVPANRWGSNVYSAPQLKIIQEVITLIVFTVFAIYYLDVKIRWNHVAA
                     FLCIIAAVAFTFLPKQ"
     gene            complement(1769..3472)
                     /locus_tag="VG754_08055"
     CDS             complement(1769..3472)
                     /locus_tag="VG754_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWF19205.1"
                     /translation="MHQQLLRVLVVEDSEDDTELMLRELRRGGYDPKFLRVETPATFN
                     KALTRKDWDLVICDYVIPGFGGLDALAIFKQKGLDVPFIVLSGLIGEDMAVRLMKSGA
                     HDCIMKDNLARLVPAVARELRDAAVRRERCEAECALRESEERFRQLAENIDSAFFMSE
                     RASDGKCSRLLYASAAYERIWGRSRESLYKDGRSWLKAVHPEDLGAVRKNLHAMERGE
                     FNQEFRILGLDLKIRWVHYRTFPVVNEEGTVYRIAGIAEDVTDRKTAEEQLASNERQI
                     AQMVEELRIIEEQLKESNQSAWESEKEMERRVHERTTGLAVANAELQNQIRARTQLEG
                     ELLEMADRERRRHGMDTHAKLSQKLMGISFMLKALERKVVHKHFLRVNETRRIQNLIN
                     EVINHSDDLAQDVGSLDLQGDDLARELKSVAANVRKTFQIACHFTVKGVLPKLPHHAA
                     SQLYKIAQDAIGNAIKHGNATMISLCLFSEPGQVRLMVKDNGLPFINTRNPGDGTGWR
                     IMHSRAGLIGATLEITPQEGQGMVVTCTLPVSKADGTAWLSPLPENGDPGLRSMVLHS
                     E"
     gene            3856..>4797
                     /locus_tag="VG754_08060"
     CDS             3856..>4797
                     /locus_tag="VG754_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19206.1"
                     /translation="MEQTKTSSFKAVPHKVRQYVGEVVYIYAFDVAYDMTRQPLRELL
                     GQPVAQFAVDASKRNPRHLFFYKPQMVRLPPLERIGPRGPVRIERTVKILPVGAISIS
                     VSVPFAVPRVEDLVDYHDIQFSNGTLQDEVRQLAEEIRRELWPHLIRPVPELVQEEAY
                     TVFCIQSPFATVDDASLSAEDWLQANRREVAALLTQEPDIDHLSRQEADESTGRYLSY
                     YEHDLVVVDWDAAMIIDQRENFHEPLYVMELANVQLAELEAYDRILDEALERSYRDLS
                     VRRVRTRGDILRELRDIRIDLARFNDELSNITKFFCDW"
BASE COUNT         1041 a         1432 c         1264 g         1060 t
ORIGIN      
        1 aaccgacaaa tgaatagcgg ccaagatgtt cgccgccctc gaccgactca aaaaggaacg
       61 attcgccatg cccgcgaatc ttgtggtacg cggaaagcgg cgtttcgaaa tcggcaagca
      121 accggcgcgt caccggaatg acgttgcctt ccttcgccag cgccaaaaat tgttcaagtg
      181 ttggtgaata catgacaatt gtccgaaagc ttaccccaaa gcgcggcgca tccgccagac
      241 agaaagcagc gtgtaaaatg cgaccgcaat ggcatacgaa acgatgccca gcccgatggc
      301 caacggaacc acttgcggca ggctgaacca aatcaacgcc gcgcccacgc cgcccgtcaa
      361 cgccgtctgc ccaagcaggc agtataaaaa gagcgacacg cgtgtttcct ggattcgcgc
      421 gatgtgccac gagcgatagc tttcctcgcc aagcgaacgc atcagccagg cgtgctggaa
      481 attgcgggcc gcaaccagaa cgctcgtcgt gctgatcatg atcgcggcca ggggccagaa
      541 ggcaaatgcg aaacacaaca ccacgttcac cgggatgccc catctccagc cgagtttttt
      601 tgccagcgga ttgccttcga gcaccaggtt gggcgtggcg acccaggtgc tgagaaaatc
      661 catcccgcgg gcaaacgcca ggagtgcgaa gaccacgcaa taaatgccgc tgccgaatag
      721 gacaaaatca tccatctatt tctttccgta aatttcgtcc ggtttcgcga gttgcggctc
      781 cgtgtttttg aaactgttgc cgccgtccgt cgcggagcgg aagaagcacg atttataacc
      841 ctcgtgacac gccgcgccaa tctgttccac ctggatcaac agcgtgtcgc cgtcgcagtc
      901 gaaggcgatg tccttgacct gctgcgtatg gccgctgctt tcgcctttca tccagaactt
      961 cttgcgcgag cggctccaga aatgcgtctt gccggtcgcg atggtggatt cgagcgacgc
     1021 ccggttcatc catgccatca tgagcacgcg cccggtggat tgctcctgga tgatggcggg
     1081 gatgaggccg tcagcattga actttagatt gtcataaatc gtgttcatca cgtcaatttc
     1141 gttggctcca aattatcagc tttcccgccg tttgcgaaca ggaagttttt cccttggccc
     1201 ctcattgttt tggcaaaaac gtaaatgcca ccgccgcgat gatgcaaaga aatgccgcca
     1261 cgtggttcca gcggattttc acgtccaaat aatagatggc aaacacggtg aacacgatca
     1321 acgtgatgac ttcctggatg atttttaatt gcggcgcgct atagacgttg ctgccccagc
     1381 ggttggcggg cacttgcaac aggtactcaa aaaatgcgat gccccaactg gcgaagatca
     1441 cctgccagat gggatggttc ttatatttca aatgaccata ccacgcgaag gtcatgaaaa
     1501 tgttcgagca ggtgagcaac acgatgggaa gccagcggga ttgaagcagc gcattcatta
     1561 tttaaatgcg gagaatgtat ccggatacgg acagaagaca aaactatttg ggaaagcctg
     1621 ccgccatcgc ctggttggag aacggagcgc ggctgtgtcc gcaggaccag ccgcagcagc
     1681 ttggaacatt cgggagttcg ttatcttcct gcggctggcg tttcgcgcac agccgcgctc
     1741 catcaatacg agatggcccg ccggaacatc actccgaatg caacaccatg gatcgcagac
     1801 cagggtcgcc gttttcggga agcggtgaca accatgcggt gccgtcggcc ttggaaacag
     1861 gcagcgtgca ggtgacaacc atgccctggc cttcttgcgg agtgatttcc aatgtggccc
     1921 cgatgaggcc cgcccgtgaa tgcatgatgc gccaacccgt gccgtcgccg ggattgcgcg
     1981 tgttgatgaa aggcagcccg ttatccttca ccatcaaacg gacctgtcca ggctcggaaa
     2041 acaggcacag ggaaatcatc gtcgcgtttc catgcttgat ggcattgccg atggcgtcct
     2101 gggcaatttt atacaattgt gaggcggcat gatgcggcaa cttgggcaat acgcccttga
     2161 cggtgaaatg gcaggcaatc tggaatgttt tccggacatt cgccgcaacg gatttcaact
     2221 cgcgcgcaag atcgtctccc tgcaaatcga gcgagccaac atcctgcgcc agatcatcgg
     2281 agtggttgat gacctcgttg atgaggttct ggatgcggcg ggtttcgttg acgcgtagaa
     2341 aatgtttgtg gaccaccttg cgttcgagcg ccttgagcat gaaggaaatt cccatcagct
     2401 tctggctcaa tttggcgtgg gtgtccatgc cgtgccggcg gcgttcgcga tcggccattt
     2461 cgagcagttc gccttcgagc tgggtgcggg cgcgaatctg gttttgtaat tccgcgttcg
     2521 ccaccgccag gcccgttgtc cgttcatgaa cgcgacgttc catttccttt tccgattccc
     2581 aggcagattg gttgctctct ttcaactgct cttcaattat cctgagttcc tccaccatct
     2641 gcgcaatctg ccgttcgttg ctggccaatt gctcctcggc agttttgcgg tcggtcacgt
     2701 cctcggcgat gcccgcgatg cgatacacag tcccttcctc attgacgacc gggaatgtcc
     2761 ggtagtgcac ccaacggatt ttgaggtcca gcccgaggat gcggaattcc tggttgaact
     2821 cgccgcgttc catcgcgtgc aagtttttgc gcactgcccc caaatcttcc ggatgcactg
     2881 ccttgagcca tgagcggccg tccttgtaaa gactttcgcg cgaacgcccc caaatccgtt
     2941 cataagccgc gctggcatac aagagccggc tgcacttgcc atcgctggcg cgctcggaca
     3001 tgaagaatgc cgagtcaatg ttctcggcca attgccggaa ccgttcctcg ctctcccgca
     3061 aggcgcattc ggcttcgcac cgttcgcgcc gcaccgccgc gtcgcgcaat tcccgcgcca
     3121 ccgccggcac gaggcgcgcg agattgtcct tcatgatgca atcgtgcgcg ccgctcttca
     3181 tcaagcgcac cgccatgtcc tccccgatca atccggacaa aacgatgaac ggcacgtcca
     3241 agcccttttg cttgaaaatc gcgagcgcgt ccaatcctcc gaatccgggg atgacataat
     3301 cgcagatgac gaggtcccaa tctttccggg tcaatgcctt gttgaatgtg gcgggggttt
     3361 cgacgcgcaa aaatttcggg tcgtaccctc ctcgacgcag ttcacgcagc attaattcgg
     3421 tatcatcttc ggaatcctca accaccagta cccttagaag ttgttggtgc atatccaacg
     3481 gagtgtcata tctaagctgt gcggtaaggg tgtcagaact ttgcaaaccc gccatggggt
     3541 aaacccctgc ccgacggggt gggtaaaccc ccaagcacct gaaaccaaat cccatcgata
     3601 tctgacagaa tgaacagagt gttcagaatg gaatggtttc ctaaaatcat atttttttct
     3661 taatattttg ttcattctgt caaaaatggt ctccctcgca ttccggcttg cgtgagattt
     3721 tccatgggta tttgggtgct tgagtatttt tgtatttgtt tggtatgcag tggcttgcgc
     3781 gttaaaaact tcgcgccaag ccgccgtgga cttccttcct ccgttggggt agaatgttac
     3841 gttatgattc cggccatgga gcaaaccaaa acttccagtt tcaaggccgt tccgcacaaa
     3901 gtcaggcaat acgtcggcga ggtcgtctat atttatgcct tcgatgtcgc ctacgacatg
     3961 acccggcaac cgctccgcga actgctcggc caacccgtcg ctcaattcgc cgtggacgcc
     4021 agcaaacgca atccgcgcca tcttttcttt tacaagccgc agatggtccg cttgccgccg
     4081 ctcgaacgca tcggcccgcg cggtcccgtt cgcatcgaac gcaccgtcaa gattcttccc
     4141 gtcggcgcca tcagcatttc ggtcagcgtt ccgttcgccg tcccacgagt cgaggacctg
     4201 gtggattacc acgacatcca attttccaac ggcaccttgc aggatgaagt ccgccaactc
     4261 gcggaagaaa ttcgccgcga actttggccg catctcatcc ggcccgtgcc cgaactcgtc
     4321 caggaagagg cttacaccgt tttttgcatc caatcccctt tcgccacggt ggatgacgcc
     4381 tcgctcagcg ccgaagactg gctccaagcc aatcgccgcg aagtcgccgc gctgctcacg
     4441 caggaaccgg acatcgacca tctttccagg caggaagccg acgaatccac cggccgctat
     4501 ctcagttatt acgagcacga cctcgtcgtc gtggactggg acgccgcgat gatcatcgac
     4561 caacgcgaaa atttccacga accgctttac gtcatggaac tggcgaacgt ccagctcgcc
     4621 gaactggaag cctacgaccg catcctcgac gaagcgctcg aacgctccta tcgcgacttg
     4681 agcgtgcgcc gcgtccgcac ccgtggcgac atcttgcgcg aactccgcga catccgcatc
     4741 gaccttgcgc gattcaacga cgaactttcc aacatcacca agttcttctg cgactgg
//
LOCUS       DASZMM010000334         5356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3986699, whole genome shotgun sequence.
ACCESSION   DASZMM010000334 DASZMM010000000
VERSION     DASZMM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3986699"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VG754_08065"
     CDS             <1..1116
                     /locus_tag="VG754_08065"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HWF19207.1"
                     /translation="SAAIGFVLAGFNGIVQAQNAPVNLPPGVQDVLKLSQAGVGEDVI
                     MAQVKNTGATYNLNADQLIYLTKSGVSQNVIRALITGNTGGVPVAAPAPVPATAPSSL
                     PPPPTTVPAAPSVETAPQVPGAIPAPQPIVVPSQGANFDSFHDQLAPYGTWMQVAGYG
                     WCWRPTMSAFDPNWHPYGNGGHWIYTEDGWNWESDYPWGNIAFHYGRWLHDSRGWLWV
                     PGYDWAPAWVTWRQTDGYVGWAPLPPGAAFRAGVGLEFNGRVGLGADFDFGLGPDAFV
                     FVGYDHFWDHDLRGLFVPRDRREWIFHHSIVMNGYRFDHGRFIVDGFGRDRIGLWTHH
                     DVHFEAPMHDYRHDHFEDRRGFHDDRDRRFERDRDHH"
     gene            1384..2349
                     /locus_tag="VG754_08070"
     CDS             1384..2349
                     /locus_tag="VG754_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HWF19208.1"
                     /translation="MPLKASSRAIASRSEQPSNIINLDEPRPAPELFSGLESNREWTP
                     YDGNTAFLLYIREVGQTKLLTPEEEAKLAARVRKGDKKAREQMIKANLRLVVKIARSY
                     EDYGMPLLDLINEGNIGLIKAVERFNPKKGAKLSTYSAWWIKQGIKRALANQSKTIRL
                     PVHVVDKLFRLRQVAVRLQELLGREPTEQELGDELGMAASKVMRLRAASVRPASLDAP
                     LGDDHNGDRIADVVKDENSFTPYEYLEEKTKTNMLHQLLDGLEPREAEILRHRFGLNG
                     SSERTLDEIGRDFGLTRERIRQIQEIALKKLRRQIEKLETVQVAA"
     gene            2404..3240
                     /gene="fdhD"
                     /locus_tag="VG754_08075"
     CDS             2404..3240
                     /gene="fdhD"
                     /locus_tag="VG754_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWF19209.1"
                     /translation="MASKNKTSRAIASAEILRLASGQSPQRARDQLAKEEPLEIRVRG
                     RSVAVTMRTPGHDKELAAGFLVSEGLIHERSDVVEIAYCQRGEAADFENTLNVFLAPS
                     VEIDFEKLTRHVFASSSCGVCGKASIEAVQQHFAPVESRMKMASSTILKLPDRLRKAQ
                     AAFAKTGGLHAAAIFDARGKLIVLREDVGRHNAVDKVIGHGFLKKLFLFDEHVLLVSG
                     RASFEIMQKALAARVPMVCAVSAPSSLAVEFAQESGQTLVGFLRGETMNVYSHPERVS
                     NG"
     gene            3314..3754
                     /locus_tag="VG754_08080"
     CDS             3314..3754
                     /locus_tag="VG754_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HWF19210.1"
                     /translation="MNTIVPLISSGVAGPLGVLHLPRLWLKTSLEARGKLAAGYPGCG
                     KGYDQMVIDGLGLKREAVLDYIKTNKPNYPQFETWVKKNGTKLDKASIDKLNSAIKGY
                     IHDDATRKGILGANGLPDDDCACKDAVNLNNLDDWKEFHEAALK"
     gene            4039..5139
                     /locus_tag="VG754_08085"
     CDS             4039..5139
                     /locus_tag="VG754_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWF19211.1"
                     /translation="MNTSDYLFPRRFTRRDFLKMAGVASASLALPSMGRTKDEPKREP
                     VRIGSGHHTYEAVEDWGQLPEGMKYGFGCGVVVDSKDNVYVTSRSANPCVAIFDRDGN
                     LIETWSKEFADEVNFTTAQVADTAHCLYLIKEGHQDVIYWTENISTNKQGRKFGARVY
                     KTDMRGKILYEIGNVEKEGSTSQKFDWTNPTDVAVAPNGDIYVVDGYGSQRVSRFDKN
                     FKHIKTIGGPGKEHGKFNTCHGVWVNTLRKAPEVYIADRANGRIEIYSMELEYKRTVL
                     NGVVRNPCCFYQHKGNLFIPDLASRVTIIDRDDKVVAQLGDGKDKKDKDTNPGLFAAP
                     HALTVDSRGDLYVVEWVGFGRPRKFKHTPQPV"
BASE COUNT         1219 a         1465 c         1533 g         1139 t
ORIGIN      
        1 tctgccgcaa tcgggtttgt tttggcgggt tttaatggta tcgtccaagc gcaaaacgcg
       61 ccggtcaatt tgcccccagg cgtgcaggac gtgctcaagt tgtcgcaggc cggtgtgggc
      121 gaggatgtca tcatggcaca agtcaagaat accggcgcaa cttacaattt gaacgccgac
      181 cagctcattt atctgaccaa aagcggtgtc tcgcagaatg tgattcgggc gttaatcacc
      241 ggcaataccg gcggggttcc tgtcgccgcg cctgctccgg tgccggcgac cgccccatca
      301 tcgcttccac caccgccaac cactgttcct gccgcgccgt cggttgaaac agcaccgcaa
      361 gttccgggtg cgattcctgc tccgcaaccg attgtcgttc catcgcaagg cgcgaacttc
      421 gacagttttc atgaccaact cgctccttac ggcacttgga tgcaggtggc cggatatggc
      481 tggtgctggc gtccaacgat gtctgcgttc gacccaaact ggcatcctta cggcaacggc
      541 ggccattgga tttacaccga ggacggctgg aattgggaat cggattatcc ttggggcaac
      601 atcgccttcc attatggccg gtggctgcac gacagccggg gctggttgtg ggtgccgggt
      661 tatgattggg cgccggcatg ggtgacatgg cgacagaccg atggatatgt cggttgggcg
      721 ccgttgccgc cgggcgcggc gttccgggcg ggtgtgggac tggaatttaa cggacgtgtg
      781 ggactgggcg cggattttga ttttgggctt ggcccggacg ctttcgtgtt cgttgggtat
      841 gatcatttct gggaccacga tttgcgcggg ctcttcgtgc cgcgcgaccg tcgagaatgg
      901 attttccatc acagcatcgt gatgaatggt tatcgcttcg accatggaag attcattgtg
      961 gatggttttg gacgcgaccg gatcgggctt tggacgcatc acgacgttca tttcgaagcg
     1021 cccatgcatg attatcgcca cgatcatttc gaagaccgcc gtggtttcca cgatgaccgc
     1081 gatcgtcgtt tcgagcgtga ccgggaccat cactgattta gattcgttcg gaacgcaaag
     1141 ttccagatgc gccagcctcc gccaattatt ggcgggggct gttgtttttg aaatagttat
     1201 tcacaattgc aaagtgcaaa ttgcgcgccc cggcgatttg cagaatgcat gaatccgtga
     1261 tttgcaagta aggtgtcgta cccgtaaatg tccggaaaac cacggaaaaa ggttttagcg
     1321 ggaggtggca cgaatctagc acaggaagcg acctgaatga aaataatcaa ccgttccaga
     1381 accatgcccc tgaaagcaag ttctcgggca atcgcttctc gttccgagca gccttcgaac
     1441 atcatcaatc tggatgagcc acggcccgcg ccggagctgt tttcaggatt ggaatcgaac
     1501 cgggaatgga cgccttacga cggcaacacc gcgttcctgc tctacattcg cgaagtcggc
     1561 caaaccaagt tgctgacgcc cgaagaagaa gccaagctcg ccgcgcgcgt ccggaaaggc
     1621 gacaagaaag cgcgcgaaca aatgatcaag gccaatctgc gattggtcgt caagatcgcg
     1681 cgttcctatg aagattacgg gatgccgttg cttgatttga tcaacgaagg caacatcggt
     1741 ttgatcaagg cagtcgaacg tttcaacccg aagaaaggcg cgaaactttc cacttacagc
     1801 gcgtggtgga tcaaacaggg catcaagcgc gcgctcgcca accaatccaa aaccatccgc
     1861 ctgccggtgc atgtcgtgga caaacttttt cgcttgcgcc aggtggccgt tcgattgcag
     1921 gaattgctcg gtcgcgaacc gacggaacag gaattgggcg acgaacttgg gatggccgca
     1981 agcaaggtga tgcggttgcg ggccgcgtcc gtccgtccgg cgtcattgga cgcgcccttg
     2041 ggcgacgacc acaacggcga tcgcatcgcc gatgtggtga aggacgaaaa cagtttcacg
     2101 ccttacgaat atcttgagga aaaaaccaaa accaacatgc tgcaccagtt gcttgacggt
     2161 ttggaaccgc gcgaagcgga aatcctgcgg catcgttttg gcctcaacgg ttcttcggaa
     2221 agaacgttgg acgaaattgg gcgcgatttt ggcctcacgc gcgagcgcat ccgccaaatc
     2281 caggaaatcg cgctgaaaaa attgcgccgc cagatcgaga aactggagac ggtgcaagtc
     2341 gcggcttaaa caccggtttt cttcctgctt gcgcgccgtg cggctttgcc cgactttcgc
     2401 cgtgtggctt ccaaaaacaa aacttcccgc gcgattgcca gcgcggaaat tttgcggctc
     2461 gcttccggcc agtcgccgca acgagcgcgc gaccagcttg cgaaggaaga gccgctggaa
     2521 atccgcgtgc gcggccgctc cgtggcggtc acgatgcgca cgccgggcca tgacaaggaa
     2581 ttggcggcgg gttttctcgt ctcggaggga ttgatccacg aacgcagcga cgtggtggag
     2641 attgcttatt gccagcgcgg tgaagcggct gattttgaga acacgcttaa tgttttcctc
     2701 gcgccttcgg ttgaaattga ttttgaaaaa ttgacgcgtc atgtgttcgc gtcctcaagt
     2761 tgcggagttt gcggcaaggc atccattgaa gcggtccaac aacacttcgc gccggttgaa
     2821 tcgcggatga agatggcgag ttcgacgatt ctaaaattgc ctgaccgttt gcgaaaagcg
     2881 caggccgcgt ttgcaaaaac cggaggactg cacgcagcgg cgattttcga tgcgcgcgga
     2941 aaacttatcg tgctgcgcga agatgtgggc cgtcataacg cggtggacaa ggtcatcggg
     3001 catggttttt taaagaaact attcctgttc gatgaacatg ttcttctcgt gagcgggcgg
     3061 gcgtcattcg agatcatgca gaaggcgctg gccgcgcgcg tgccgatggt ttgcgcggtg
     3121 tccgccccgt caagtctcgc cgtggaattt gcacaggaga gcgggcaaac gcttgtcggt
     3181 ttcttgcgcg gcgaaacgat gaatgtttat tcgcacccgg agcgtgtctc gaacggttaa
     3241 atttccgttg accattctcc gcgcctgttt ctattctgaa tgcccattac aaaaccacaa
     3301 aaaaccaaac aatatgaaca caatagttcc tctcatcagt tctggagttg cgggtccgct
     3361 cggcgtgctg catctgcccc gcctttggct caagacatcg ctggaagccc gcggcaaact
     3421 cgccgccggt tatcccggct gcggcaaggg ttacgaccag atggtcattg acggtcttgg
     3481 actcaagcgc gaagcggtgc tggattacat caagaccaac aagcccaatt atccgcagtt
     3541 cgaaacctgg gtcaaaaaga acggcaccaa gctcgacaag gcttcaatcg acaaattgaa
     3601 ttccgccatc aagggctaca tccatgacga cgccacgcgc aagggaatcc tcggcgccaa
     3661 cggcctgccc gacgatgact gtgcctgcaa agacgcggtg aatctcaaca atctcgacga
     3721 ctggaaggaa ttccacgaag cggccttgaa ataagcgagc aactcgtttt tcaaccggtg
     3781 ccagcacaag ctagcgccgg tttttttgta attggaccgc ggctgcctca gccgcagcaa
     3841 tagatgaaag tcctgtgcca tattgtcatt ggtgggcagc aacaggccaa gcgcacacga
     3901 aatattgaat ccttccaaaa aatcacacct tgctgcggct gggacagccg cgctccaaaa
     3961 tcgcgctgct tccctctcga catgagcagc atttaatcct atcttttgaa gcgataaacc
     4021 cataaaagtt ggcataacat gaatacgagt gattaccttt ttccccggcg atttacccga
     4081 cgtgacttcc tgaaaatggc cggcgtggca tccgccagcc tcgcattgcc cagcatgggg
     4141 cggacgaagg atgaacccaa acgcgagcca gtccgcatcg gcagcggtca tcacacttac
     4201 gaagcggtcg aagactgggg acagcttccc gaagggatga aatacggttt tggttgcggc
     4261 gtggttgttg attccaagga caacgtgtat gtgacatcgc gttcggcgaa tccttgcgtt
     4321 gccatctttg atcgcgacgg caacttgatc gaaacctgga gcaaggaatt tgcggacgag
     4381 gtgaacttca cgaccgcgca agtcgcggac accgcgcatt gcctttacct catcaaggaa
     4441 ggccatcagg acgtgattta ttggacggag aacatcagca cgaacaagca gggacgcaag
     4501 ttcggcgcgc gcgtttacaa gaccgacatg cgcggcaaaa tcctgtatga aatcggcaac
     4561 gtcgagaagg aaggttccac ttcgcaaaaa ttcgactgga ccaatcccac cgatgtcgcg
     4621 gtcgcgccga acggggacat ttatgtcgtg gacggttacg gaagtcagcg cgtgagccgg
     4681 ttcgacaaaa atttcaaaca catcaaaacc atcggcggac cgggcaagga acacggcaag
     4741 ttcaacacct gccacggagt ttgggtcaat acgttgcgca aggcgccgga agtgtacatc
     4801 gccgaccgcg ccaacggacg catcgagatt tattcgatgg aactggaata caaacgcacg
     4861 gtgctcaatg gcgtcgtgcg caacccctgc tgtttttacc agcacaaggg caatcttttc
     4921 atccccgacc ttgccagccg cgtgaccatc attgaccgcg acgacaaggt ggtggcgcag
     4981 ttgggcgacg gcaaggacaa gaaggacaag gacaccaatc ccggactgtt cgccgcgccg
     5041 cacgcgctga cggtggattc gcgcggagat ttgtatgtcg tcgaatgggt cggattcggc
     5101 cgtccgagaa aattcaagca cacgccgcag cccgtataaa cgagccgccc cggaccgcgg
     5161 gtccggcgac ccgcagcaat gcacgatttg caacccgcgt ccgattgtca attttggcaa
     5221 cgccttacaa ttggacgttg ctgcggctca tcgagccgcg ctccgctcac tctcacgttg
     5281 ccattatcgc acccggaatt tatcgtgata ttatcgctcc gtggatgggc gtgggatacg
     5341 gtgtgttcac tccttt
//
LOCUS       DASZMM010000335         4600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_424971, whole genome shotgun sequence.
ACCESSION   DASZMM010000335 DASZMM010000000
VERSION     DASZMM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424971"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VG754_08090"
     CDS             <1..441
                     /locus_tag="VG754_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19212.1"
                     /translation="KQAQVELIGAFPEVWVSRSGLGQALCHLLDNSLKFMAPGVKPRI
                     QFQSEERGRFLRLKIQDNGIGVEAAYHERIFQPFERLQGDAYEGTGIGLAIVQEAVHR
                     MGGKAGVDSELGKGSCFWMDLPRVSDAAEENIPARELEAQVHSA"
     gene            480..932
                     /locus_tag="VG754_08095"
     CDS             480..932
                     /locus_tag="VG754_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19213.1"
                     /translation="MYLTTSNPSKQLLLLTFTGQVTAEELAAGRENMMELLDGLKPGF
                     RLLSDLTMLESFDEGCAAEIAQNMHLFSEKGVAMVVRVIPDESKDFGLNILSLFHYPQ
                     RPNVVTCSTMIEAVKALALQPVPIINRNLLMFYIAAAETAAFQIFHRF"
     gene            1118..1726
                     /locus_tag="VG754_08100"
     CDS             1118..1726
                     /locus_tag="VG754_08100"
                     /inference="COORDINATES: protein motif:HMM:NF021998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HWF19214.1"
                     /translation="MFTAIIVVLRTLRTVFTAEFFAGVVHWFEDAYVREDTPLIGRFV
                     ARPNIVHHHYPRHMTRNSWWESSWDLCLLSALLVVIAWCCGVLTWQIVLFAVLAANAN
                     QMHKWAHRTRPENGAFISFFQDMRILQTAQHHALHHTDPKNSHYCTMTNALNPVLDGI
                     RFWDGVEWLLARTIGLQRRPDTSVRGHGPGPAWLLEYRRPSK"
     gene            1836..2273
                     /locus_tag="VG754_08105"
     CDS             1836..2273
                     /locus_tag="VG754_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19215.1"
                     /translation="MSQPVEILVVEDYEPDAHLLQLLFRRNHILNQVHVVTDGSAAID
                     YIFARGGHTSRATEPLPVVVIMDIRLPKVDGWEVLRQLRNDPRTQDLPVIIMTGSLYE
                     RELEIAVGLGANACISKPVQIDELCEAFKKCGLNWAITTQPSA"
     gene            2475..2747
                     /locus_tag="VG754_08110"
     CDS             2475..2747
                     /locus_tag="VG754_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HWF19216.1"
                     /translation="MAQAIMDPEEVRRFAQELKKFNADLQNGMAALQARFGALGDSWQ
                     DQEHAKFSEEFKQTMKALKKFIEVSNAHTPYLLRKAQRIEEYLNQH"
     gene            2756..3337
                     /locus_tag="VG754_08115"
     CDS             2756..3337
                     /locus_tag="VG754_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19217.1"
                     /translation="MAIRANITSVEAIESFRANLIVYLSKAKPCLEEANAEIVRTRIW
                     LQTVQRVHWEGVHKKCLRDLEEAKAALFSSKLSKLREISSAEQLAVTKAKRKLDEAEA
                     KLRIIRRWDREFENLIEPMSRKLEKMQVVLMDDLQNAVVSLAQTVEMLDAYARVTTSS
                     VGDGSSPSGSSSESKSAANVPETVSSEKKGDDA"
     gene            3334..3546
                     /locus_tag="VG754_08120"
     CDS             3334..3546
                     /locus_tag="VG754_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19218.1"
                     /translation="MSLRASGNQLAALTKELLLQWDDTKSYWKDSKSGEFEKKYLEEL
                     QANVDSTVVVVEQLDKLMTKIRKDCE"
     gene            3539..>4600
                     /locus_tag="VG754_08125"
     CDS             3539..>4600
                     /locus_tag="VG754_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19219.1"
                     /translation="MSSHPGVKKTLELLENLRAALADCAAAEEKLSHEFRIRLGKERQ
                     RREELTDEEAKRVATETAEAQEAYHSAKEAEDARYAARKACINRARASSKEQTLERIE
                     NRTGNRKYELQRLLMESERKRDADFANAGTEYQEFNANLAGELGALDALEKKARNSFR
                     GYGSFRRLISRAQETALVNPAPDQNQLMGEMRTAINKASGELDRFRRLIIPMLFSFLP
                     IWLLLPLCATPLVLQHFKIGSLTQTEAIGISIGAAVAISVLYFLGKGRGSSSAKIIAD
                     ALGKARKLYTACLEKSEAHYREENQRIQNEFTVNTEIIDAELKAALKNASAQRGSFRE
                     AIDDKTVRITSKNEQRHRAK"
BASE COUNT         1103 a         1188 c         1304 g         1005 t
ORIGIN      
        1 aagcaagccc aagtcgaatt aatcggggcc tttccggaag tgtgggtttc tcgaagcgga
       61 ttgggccagg cgctttgtca tctgctggat aattccttga aatttatggc ccccggagtg
      121 aaaccgcgca ttcaatttca atcagaagaa cggggacgtt ttctgcgatt gaaaatccag
      181 gataacggca tcggagtgga agcggcatat cacgaacgca ttttccagcc atttgaaagg
      241 ctgcaaggag acgcttacga gggaacggga attggattgg ccattgtcca ggaggcggtg
      301 catcggatgg gtgggaaagc aggggttgat tccgagcttg gcaaagggag ctgtttttgg
      361 atggacctgc cccgcgtttc tgatgccgcg gaggaaaata ttccagcacg ggagttggaa
      421 gcacaggtac attcggcttg acgtcggtgt attcgccagc tcggataaaa ccggatgata
      481 tgtatctgac aaccagtaat ccctccaagc aactgttgct tttgactttc actggtcagg
      541 tcacggctga agaattggcg gcaggacgcg aaaacatgat ggagcttttg gacggattga
      601 agccgggatt tcgcctgctt tcggatctca ccatgctgga gtcatttgat gagggttgtg
      661 cagcggagat agcccaaaac atgcatctgt tcagcgagaa aggcgtggcg atggttgttc
      721 gcgtgattcc tgacgagagc aaggactttg gactgaacat cctttcgctt ttccattacc
      781 cgcaacgtcc gaatgtggtc acctgcagca cgatgattga agccgtgaag gcgctggcgc
      841 ttcagcccgt gcccatcatc aatcgaaacc tcctgatgtt ctacatcgct gcggctgaga
      901 cagccgcgtt ccaaatcttc catcgctttt aacgatggat ttcttctttt tatcgattca
      961 cgcctttcct ccagattgag ttacaacgca attgtgcaaa ttgaaaacgc acaaccgacc
     1021 ggagaatgtg gcgctcgcgc cggaaagaca atcaaccaca ccagcaatgc cgacgcgggc
     1081 gctctccggt cgggcaaatc caactcgaag gaaccttatg tttactgcca tcatcgttgt
     1141 cttgcggact ctgcgaaccg tgttcaccgc cgaattcttt gccggcgtgg tgcattggtt
     1201 tgaggacgct tatgtgcgcg aagacacgcc gttgatcggt cgttttgtcg cgcggccgaa
     1261 catcgtgcat catcattatc cgcgccacat gacgcgcaat agctggtggg aatcttcgtg
     1321 ggacctgtgc ctgctgtcgg cgttgctggt ggtcatcgcg tggtgttgcg gcgtgctgac
     1381 atggcagatt gtgctgttcg cggtccttgc ggcgaacgca aaccaaatgc acaaatgggc
     1441 gcatcgcact cgcccggaaa acggcgcgtt catttcattt ttccaggaca tgcgaatcct
     1501 gcaaaccgcg caacatcacg ccctgcatca taccgatccc aagaacagcc attattgcac
     1561 gatgacgaat gcgttgaatc cagtcctcga cggcattcgt ttttgggacg gtgtggaatg
     1621 gttgctggcg cggacgattg gcctgcaacg ccgtcctgat acatcggttc gcggtcacgg
     1681 gccaggtccg gcgtggcttc tggagtatcg caggccgagc aaatagtctg agttacacga
     1741 cattgagggt gacgccttaa gcagaatcac tggaaggatt ggcaagctgg ttgctcttac
     1801 atatttgggg gcaaatgacg tatttgcccc cgtttatgtc acaacctgtc gagatacttg
     1861 tggttgaaga ttatgaacct gatgcgcatt tattgcagct cctgttcagg cgcaaccaca
     1921 ttttgaacca agtgcatgtc gtcacggacg gttccgcggc aatcgactat atttttgcgc
     1981 gtggaggcca cacttctcgc gcgacagagc cgcttccggt ggttgtaatt atggacattc
     2041 gcctgccaaa agtggacggt tgggaggtat tgcgccaatt gcgtaacgac ccgcgaacgc
     2101 aggatttgcc ggtcatcatc atgaccggtt cgctctacga aagggaactc gaaatcgcgg
     2161 ttggattggg cgcaaacgcg tgcatttcca agccggtcca gatcgacgaa ttgtgtgaag
     2221 ccttcaaaaa gtgcggtctg aactgggcca tcacaacgca gccctcggcg tgatcggaat
     2281 cgttttgcct gcttgatcgg agcggcaaca tcttcaagga agatttcgca cccgtttttc
     2341 ccgccttcaa tcctcaacca ttatcgtttg cgtaccgggc caaaaacagg cactataagg
     2401 caagctgaaa cgctgggcgc cgggtcgctt gactttttgg cgagtcataa ccataacgaa
     2461 caagagacat tgatatggcc caggcaatca tggatccgga ggaggtgcgg cgcttcgcac
     2521 aggaattgaa aaagttcaac gccgatttgc agaacggcat ggccgcgttg caggcgaggt
     2581 tcggcgcgtt gggcgattcc tggcaggacc aggagcacgc gaagttctcg gaggaattca
     2641 agcagaccat gaaggcgctc aagaaattca tcgaagtttc caacgcgcac actccttatc
     2701 tcctgcgcaa ggcccagcgc atcgaggaat atctgaacca gcattgagtc gttccatggc
     2761 catccgcgcc aacatcactt cggtcgaagc cattgaatct ttccgggcaa acctcatcgt
     2821 ctatctgagc aaggccaaac cgtgccttga agaagcaaac gcggaaatcg tccgcacccg
     2881 catctggctt cagaccgtgc aacgagtcca ttgggaaggc gtccacaaga aatgtcttcg
     2941 cgaccttgag gaggcgaagg cggctttgtt cagttccaaa ctttccaagt tgcgcgaaat
     3001 ttcatccgcc gaacaactcg ccgtcaccaa ggcgaaacgc aagctggacg aagccgaggc
     3061 caagctgcgc atcatccgcc gctgggaccg cgaatttgaa aacttgatcg aaccgatgtc
     3121 gcggaaattg gagaagatgc aggtcgtgtt gatggacgat ttgcagaatg cggtggtttc
     3181 attggcgcaa acggtggaga tgctggatgc ctacgcgcgc gtcaccacgt ccagcgtggg
     3241 cgacggttcc agtccttccg gttcgtccag cgaatcgaaa tccgccgcca atgttccgga
     3301 aacagtttct tcggaaaaaa aaggagacga cgcatgagct tgcgcgcttc tggaaaccag
     3361 cttgccgcgc tcacgaagga gcttctcctg caatgggacg acaccaaatc gtattggaaa
     3421 gattccaaga gcggcgaatt cgagaaaaaa tatttggaag aactgcaggc gaatgtggat
     3481 tcgaccgttg tcgtcgtcga gcagttggac aaattgatga ccaaaatcag gaaggattgt
     3541 gagtagtcat cccggagtaa aaaagacgct tgagctgctc gaaaatttgc gggcggcgct
     3601 ggccgattgc gccgccgccg aggagaagtt gtcccatgaa tttcgcatcc ggctcggcaa
     3661 ggaacgccag cgccgggaag agttgacgga tgaggaggcg aagcgggtcg ccacggaaac
     3721 cgccgaagcg caggaagctt atcacagcgc gaaggaagcc gaagacgcaa gatacgctgc
     3781 gcgcaaggcg tgcatcaacc gcgcgcgcgc ctcaagcaag gaacaaaccc ttgaacgcat
     3841 cgagaatcgc accggcaacc gcaaatacga gttgcaaaga ttattgatgg aatcggaacg
     3901 caagcgcgac gccgattttg caaacgccgg gacagagtat caggaattca acgccaattt
     3961 ggcgggcgaa ctcggcgctt tggacgcgct cgaaaaaaag gcgcgcaatt cgtttcgcgg
     4021 gtatggaagt tttcgccggc ttatcagtcg cgcgcaggaa accgctctag tcaacccggc
     4081 gcctgatcaa aaccaattga tgggtgaaat gcgcacggcg ataaacaagg cgagcggtga
     4141 actggatcgt tttcgccggc tcatcatccc gatgctgttc agtttcctgc cgatttggtt
     4201 gctcctgccg ctctgcgcaa caccgctcgt gcttcagcat tttaaaatcg gctcgctgac
     4261 gcagacggag gcaattggca tcagcatcgg tgccgcggtt gccatttcgg tcctttattt
     4321 tcttgggaaa ggccggggtt cgtcgtccgc gaaaatcatt gccgatgcgt tgggcaaggc
     4381 gcgcaaactt tacacggcct gtcttgaaaa atccgaggcg cattatcgcg aagaaaacca
     4441 gcgcatccaa aacgagttta ctgtcaacac cgagataatt gatgctgagt tgaaagcggc
     4501 tttgaagaac gcaagcgcgc agcggggttc tttccgtgag gcgattgatg acaagaccgt
     4561 ccgcatcacc tcgaaaaacg agcaacgtca tcgcgccaaa
//
LOCUS       DASZMM010000336         5126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2055563, whole genome shotgun sequence.
ACCESSION   DASZMM010000336 DASZMM010000000
VERSION     DASZMM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055563"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(746..1135)
                     /locus_tag="VG754_08130"
     CDS             complement(746..1135)
                     /locus_tag="VG754_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19220.1"
                     /translation="MKIQAPFKTSPDERIRMIQCSLRCLAFGAASMALTLGLPFAVGL
                     VINLPPPFRDDGLLAICWIPVVSFTFSLLAWMNVFHAQSAAHGEWNPAAAQLIWGRVF
                     SAIGVLMSLVSAGFFFCFLLKLFPWQG"
     gene            complement(1150..1353)
                     /locus_tag="VG754_08135"
     CDS             complement(1150..1353)
                     /locus_tag="VG754_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19221.1"
                     /translation="MKIPNTSFGGRRRLSGSAVAVMLALVGMMLVFVAANTVAIRSLQ
                     RELKLIEKKQVQRLQTASPAASK"
     gene            complement(1358..1861)
                     /locus_tag="VG754_08140"
     CDS             complement(1358..1861)
                     /locus_tag="VG754_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19222.1"
                     /translation="MKTNSLYSSRRECGIMLVDCLVYIAVFFMILGVAFKMFYSCWDS
                     AKTFRRNSDQIAATLKTGERWREDVRRATATPRAEESSEGTVLRIPQKTSEVDYRFFD
                     GELWRRDGNAGEWTPLLLKVKSSRMEQDKREHVAAWRWEVELATRRKGAKVVPLFTFE
                     AVPQRVD"
     gene            complement(1858..2250)
                     /locus_tag="VG754_08145"
     CDS             complement(1858..2250)
                     /locus_tag="VG754_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19223.1"
                     /translation="MVTRTSQKFAGRPKTESGSLVTELVVAMAVLVLAIIPLAFTYPQ
                     EEKLLRSSYQRAVAMEIVDGEMEILLAGEWRSFKEGAQPYTFRAQSATNLPPGRAELT
                     VAGKHLRLDWQPAKKASGGEVVREADVK"
     gene            complement(2244..3404)
                     /locus_tag="VG754_08150"
     CDS             complement(2244..3404)
                     /locus_tag="VG754_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF19224.1"
                     /translation="MNWTFYLLQLPLTLLVWVIFWLGPFAFALWLGYYFMSLRLRRRE
                     RARFFIDMVERGLKEGRRPEDTVVSLARNRDAAMGPHFHLVAAYLEKGLSLHDALVKV
                     PQLLPPQISAMLQTGLRLGDVEKVLPACRRLFKDAVSQTRGAINYLMLLAFVGLPTNI
                     VLFTIMTIYVLPQFQAIFIGMEIPPGHGFAFLMGHRGLIVALQILLLLFVWIAAFIYL
                     DGPRIFPWLERLTKPLLDRIFYALPWRRKRMQRDFSGMLAILLDSGMPEGEALTAAAD
                     CTANQIFREGTKRAVAALQNGMKLPEAVQMVDDSGEFRWRLTQALHAHSGFFRAISGW
                     NESLDAKAFQEEQATAQTVTTGIVVLNGVLVGVIVVSIFSMLISLINSAVLW"
     gene            complement(3420..4397)
                     /locus_tag="VG754_08155"
     CDS             complement(3420..4397)
                     /locus_tag="VG754_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF19225.1"
                     /translation="MKYDEFAFFNQQLAAMLREGIPLEGALRQLSANMRQGGLRAEVE
                     KLETDLRNGVPLNQALASRKLPEFYVQTLQVGVAGNDLPGVLTLLADYYQRVDATWTR
                     LKGLMVYPLLVLIAAFVLSCFLTFACLQILGSGFPEAMGFNMPPTVLLNLWAPPVLIG
                     LLLTLALLFALVPSWHRRLRWRVPAFREAKLAQVASAMALMLKSGGNLNDSLGLVRQM
                     EHDTIASAELSQWQARLANGRGQFDELAEPGRAFPPLFLWMIANAGEDLAGGFRRASE
                     IYSARASQNTEMFLYAALPISVLGLGFMIICQIFPLIHGFTSMLNAIGS"
     gene            complement(4394..>5126)
                     /locus_tag="VG754_08160"
     CDS             complement(4394..>5126)
                     /locus_tag="VG754_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWF19226.1"
                     /translation="ASGTKSLDELGLPEHTTAELERFLRQTSGLLLLTGPAGSGKTTT
                     IYACLRFLSQLGGRHIITVEDPAEQIIPGIMQTEVNEARGLTFGMATRHLLRQDPQVL
                     VIGEIRDEETANIAVRAALTGHLVISTLHAGSCRGVFERLQVLCADHSAVASAIELVF
                     NQRLIRRLCRECHGAGCNACLSTGFQGRAPLIEWLKVDQNLRGAIRRQELDQLAPQQS
                     LETSARELLRQGVTNDAEYERIFGL"
BASE COUNT         1202 a         1475 c         1430 g         1019 t
ORIGIN      
        1 gtcaagggtg cgggtcggct tgccgaacgt ggtgatgacc accacggtgg acttgcgcac
       61 ctgcacacag gaaaggatgg caaggcaaat caccgcgagc aacacgccgg tgcctagtgt
      121 gaaataattt cgtttcataa cagagaattg atcaggaact caaaattgcg acaagacgtc
      181 gagtattcac gctcgccctc accccagccc tctcccccgg ggagaggggg aaccatctgg
      241 cggtgtttgg acgaatagcg cctcactgtt gtcttccgct tcgtgaaatt tctaaagaga
      301 cggcaattgg aaccagaata agggaaattt tcgcgatgga cgaaacgccc tccctctcct
      361 ggccgagagg gggaaccttg ggcgcatctc agtttcctgc acgacggagt aattgaacgt
      421 ttcctccccc tcaccccggc cctctccctt ggggagaggg agcaacgcat cccgtcgtgg
      481 ttgcaatacg atggcgttcg atttgtcgag cagcggccga atggttcccc ctctccttgg
      541 ggaccacaca acgcgaagcg ttcgtctctt ttggggaaac tgacttgtgg cgggcttggg
      601 aagccattgg gccggggtga gggggacagc gatgtgcgcc ggcccatgaa cacaattcgc
      661 aagagcccat aaacgtgcaa aatagtgaga tgcgcccgcg ggcgggaaga gatggcgaat
      721 ttcgagattc agaagatgat ttgcactaac cctgccaggg aaacaatttc aaaaggaaac
      781 aaaagaagaa gcccgccgag acaagtgaca tcaaaacgcc gatcgcgctg aagacacgac
      841 cccagataag ttgagcagcg gcggggttcc attctccgtg ggccgcgctt tgggcgtgga
      901 acacattcat ccaggccagc aacgaaaacg tgaagctcac aaccggaatc caacagatgg
      961 ccaaaagccc gtcgtcacga aacggcggcg gcagattgat gacgagtccg actgcaaaag
     1021 gaaggcccaa tgtcaacgcc atgctcgccg cgccaaatgc caggcagcgc aacgaacatt
     1081 ggatcatgcg gatgcgttca tcggggcttg ttttaaacgg cgcttgaatc ttcatgcgca
     1141 acacgaattt tattttgacg ctgcgggcga tgccgtttgc agccgttgca cctgcttttt
     1201 ctcgatcagc ttcaattctc gttgcagact cctgatggca actgtatttg ccgcgacaaa
     1261 caccagcatc attcccacca atgccagcat cacggcgacc gccgagccgg atagtctgcg
     1321 ccgcccgcca aaagatgtgt ttggaatttt cataagatca atcaacgcgt tgcggcaccg
     1381 cctcgaacgt gaacaagggc acaacctttg cgcctttgcg acgcgtggca agttcgactt
     1441 cccagcgcca cgccgcgaca tgttcgcgtt tgtcctgttc catccgtgaa gattttacct
     1501 tcagcaacag cggagtccat tcgcccgcat tgccgtcgcg acgccaaagc tcaccatcaa
     1561 aaaaccggta atccacctcg ctggtttttt gaggaatgcg caacactgtt ccctcggacg
     1621 attcctcggc gcgcggcgtc gcggtggcgc ggcgcacgtc ttcacgccaa cgctcgcccg
     1681 ttttgagcgt ggcggcgatt tgatcgctgt tccgtcgaaa tgtcttggcg ctgtcccagc
     1741 aactgtaaaa catcttgaac gcgacgccca aaatcatgaa gaaaaccgcg atgtaaacca
     1801 aacaatccac cagcatgatg ccgcattctc ttcgcgaaga ataaagagag tttgttttca
     1861 tttcacatcc gcctcccgca caacttcgcc tcccgaagct ttctttgccg gctgccaatc
     1921 caggcgcagg tgcttgcccg cgaccgtcaa ttccgcgcgg cccggcggca agtttgtggc
     1981 tgattgcgcg cggaaggtgt agggttgcgc gccttccttg aacgaacgcc attcgcccgc
     2041 gagcaaaatt tccatttcgc cgtccacaat ctccatggcc acggcgcgtt gatatgaact
     2101 gcgcaacaat ttttcctcct gcggataagt aaaagccagc ggtatgatgg cgaggacgag
     2161 gaccgccatg gccaccacca attccgtcac aagcgagccc gattccgttt tcggccgccc
     2221 ggcaaatttt tgagaggttc gtgtcaccat aacactgccg agttgatgag cgatatgagc
     2281 atggaaaaaa tgctgaccac gatgacgccg accagcacgc cgttgagcac cacgatgccg
     2341 gtggtgacag tttgcgcagt ggcttgttct tcctggaatg ccttcgcgtc gagcgattcg
     2401 ttccagccgg aaattgcgcg gaaaaagccg ctgtgcgcgt gcagcgcctg tgtcagccgc
     2461 caacggaatt cgccgctgtc atccaccatc tgcaccgctt ccgggagctt catcccgttc
     2521 tgcaacgcgg caacagcgcg ttttgtccct tcgcggaaga tttgatttgc cgtgcaatcg
     2581 gctgctgctg tcaatgcttc cccttccggc attccggagt ccagcaagat ggccagcatt
     2641 ccggagaaat cccgttgcat ccgttttctc cgccatggca gcgcgtaaaa aatgcggtcg
     2701 agcaaaggtt ttgtgaggcg ttccagccat ggaaaaatgc gcgggccatc gaggtaaatg
     2761 aacgcggcga tccagacaaa caaaagcaat aagatttgca atgcaacgat gagcccgcgg
     2821 tggcccatca aaaaagcaaa accgtgaccc ggcgggattt ccattcctat gaagatggct
     2881 tggaattgcg gcaacacata gattgtcata atcgtaaaaa gcacaatgtt ggtcgggaga
     2941 ccgacgaacg cgagcagcat caaatagttg attgcgccgc gcgtttgcga gacggcatcc
     3001 ttgaacagcc ttcgacatgc gggaagaact ttttcgacat cgccgagccg caagccggtt
     3061 tgcagcatgg cggaaatttg cggcggcaaa agttgcggca cctttaccaa agcgtcatgg
     3121 agcgaaagcc ctttttcgag ataagccgcc acgagatgga aatgcgggcc catggcggcg
     3181 tcgcgattgc gcgcaagtga aacgacggtg tcctcgggcc ggcggccttc cttgagtccg
     3241 cgttcgacca tgtcgataaa gaagcgggcg cgttcacgcc ggcgcaagcg caggctcatg
     3301 aaataatatc cgagccagag cgcgaaggcg aacggcccca gccagaagat cacccacacc
     3361 agcagcgtca gcggcagttg cagcagataa aatgtccagt tcatatcggt tcttttgagc
     3421 tatgagccga tggcattcag catggaggta aatccatgta tcaacggaaa aatctggcaa
     3481 atgatcatga atcccaatcc gagcaccgaa atcggcagcg cagcatacaa aaacatttca
     3541 gtgttctggc ttgcccgcgc gctgtaaatt tcagatgcgc ggcgaaatcc tccggccaaa
     3601 tcttcaccgg cgttggcgat catccagaga aacagcggcg gaaacgcgcg gccgggttcg
     3661 gccagttcgt cgaattggcc gcgtccattg gcgagccgcg cctgccattg cgacaattcc
     3721 gcgctggcaa tcgtgtcgtg ttccatctgc cggacaaggc cgagcgaatc attgagattg
     3781 ccgccggatt tgagcatcaa cgccatcgcc gaagcgacct gcgccagctt ggcttccctg
     3841 aacgcgggaa cgcgccatct caatcgccga tgccatgatg gcaccagcgc aaacaacagc
     3901 gccaaggtca gcagcaaacc gatcaacacc ggcggcgccc aaagattcaa taagacagtt
     3961 ggcggcatgt tgaaacccat cgcttccgga aagccggagc cgaggatttg caggcaagcg
     4021 aacgttagaa agcaggagag cacaaacgcg gcgatcaaga cgagcagggg atagaccatc
     4081 aatcctttca accgcgtcca tgtggcatcc acgcgctggt aataatccgc caacaaggtc
     4141 aagacgcccg gcaaatcatt tccggcaacg cccacttgca gcgtttgcac ataaaattcc
     4201 ggcagcttgc gcgaggcgag cgcctggttg agcggcacgc cgttgcgcaa gtcggtttcg
     4261 agtttttcga cttcggcgcg caatccgccc tggcgcatgt tcgcggacaa ctgccgcaac
     4321 gcaccctcaa gcggaattcc ttcgcgcagc atcgccgcaa gctgctggtt gaagaacgca
     4381 aactcgtcgt atttcataag ccgaaaatcc tttcgtattc cgcatcgttg gtgacgccct
     4441 gccgcaacaa ctcgcgagcg gaagtttcga gcgattgttg cggcgcgagt tggtccaatt
     4501 cctgacggcg gattgcgccg cgcaaattct ggtccacctt gagccattca atcaatggag
     4561 cgcgcccttg gaacccggtt gagagacacg cgttgcaacc cgcgccatgg cattcgcggc
     4621 aaagacggcg gatcaatcgc tggttgaaaa cgagttcgat ggctgaagcc acagccgaat
     4681 ggtccgcgca aaggacttgc aaccgttcga acactccgcg acatgaaccg gcgtgaagcg
     4741 ttgaaatgac gagatgcccc gtcaacgctg cgcgcacggc aatgttggcg gtttcttcgt
     4801 cgcggatttc gccgatgacg agcacctgcg ggtcctggcg caaaagatgc cgcgtggcca
     4861 tgccgaaggt cagtccgcgc gcttcgttca cctcggtttg catgatgccg ggaatgattt
     4921 gttccgcggg atcttcaacg gtgatgatgt gccgtccacc cagctgcgac agaaaacgga
     4981 gacaagcata aatggtggtg gttttgccgc tgccggcggg accggtgagc agaagcaggc
     5041 cggaagtttg acgcaaaaag cgttcaagtt cagccgtggt gtgttccggc agaccgagtt
     5101 catcgagcga tttcgtgccg ctggct
//
LOCUS       DASZMM010000337         5173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4809858, whole genome shotgun sequence.
ACCESSION   DASZMM010000337 DASZMM010000000
VERSION     DASZMM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4809858"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VG754_08165"
     CDS             complement(<1..1091)
                     /locus_tag="VG754_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19227.1"
                     /translation="MPLQDLTPQLRTRLNRMERAVGWFIMLATLLLLVGLGYYIYKAA
                     ESRGWFKIKAQYFVYTERGDGLAVGGPVNLMGFNVGRILEIKPMPPMWGQIVTNNVYV
                     KFEVLEPNFGYIWTDSSRVSIKSGFLNARQLDISKGLIGYATYISYGFRNDMTVAEAK
                     ALPHPEEWRLGQEIRDGTNLVLGAWKPLTNNWNKLSEQLGSNTFCAINPNVTKRYITA
                     VWNDDRRSYELFKGTNIYGLPRYEEPALNDELQSMVLQVKAALPGILDLTNKIDRVLA
                     NTSDLTSNLNAIAANARPAVANLNAITERLKDPNGSLGEWVLPTNINQKLDSTLQAAG
                     GTLTNLDTNLMTLNLTLQNLANITSNLNN"
     gene            complement(1289..2074)
                     /locus_tag="VG754_08170"
     CDS             complement(1289..2074)
                     /locus_tag="VG754_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF19228.1"
                     /translation="MEPEASKYSSTAPIIEMQGVSVGSIMDLTQITLEEVNWTVNAGE
                     FWVIAGMHNSGKTDLMWMTAGIMPPQNGTYRLFGHEMPMYSEEQLSERLRLGLVFENG
                     QLLRHLNVHENIALPLHYHREIEWTEAEARVKAMLETAELTPYADVMPGLLGRNWQKR
                     GGLARALMLEPEVLLLDHPLGGLDFRHANWWLNFLRNWHAQQTAERPRTVIVTAEDLR
                     PWKNFDSHFAILKKQRFIPLGHRPKFANHQEPLVNELLAEQLQ"
     gene            complement(2077..2943)
                     /locus_tag="VG754_08175"
     CDS             complement(2077..2943)
                     /locus_tag="VG754_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF19229.1"
                     /translation="MSSGRFSALYASRARALMRAPLPKAYSNYFGRQLIRLLITVHGL
                     GAFALITLGVILTKLTTAESVTWPAIFRELRRSGLRLIPMFLFMSGVLGLLVIGQTLT
                     LLFHYQVGSTNVIGTIMVAVVVRELGPLITAMLVLSRSGTANVIELSTARALGEVEAL
                     EALGIDPVHYFVMPRVIGMALGMFSLTIYFIMGALVSGYLWAFLQNVPLSPAEYFEQL
                     AHALNGMDFVILALKSSLFGVLIAVITCYHGLAQPLRLEEVSQATVGAVTQSIVACVA
                     LDAVFIFVYLVT"
     gene            3098..3631
                     /locus_tag="VG754_08180"
     CDS             3098..3631
                     /locus_tag="VG754_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWF19230.1"
                     /translation="MKNVVYFDLETQKSADEVGGWDKISAMRMSVGVTYSTTRGDYRI
                     YGEKQVDDLLTELQRADLVVGFNHLRFDYEVLHGYTSLDLRQLPTLDMLVELQTTLQH
                     RLSLDSIATATFGVEKTAEGLQAIQWFREGRLMDVAEYCCYDVKLTKLVYEYGANHRQ
                     LHYHNKFGKKLTVPVKW"
     gene            complement(3679..4080)
                     /locus_tag="VG754_08185"
     CDS             complement(3679..4080)
                     /locus_tag="VG754_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWF19231.1"
                     /translation="MTFLDTGIMVGALLKSHPEHEACIAALEDSAQPFTDAHAMAETF
                     ATLTGFYKVPTDAAAELTLSLRGVVVVEALALADYETAIREARSRGVMGGGIYDSLHA
                     TFARRKKAARIVTRNPSNFQHVAPDIEIVIP"
     gene            complement(4077..4325)
                     /locus_tag="VG754_08190"
     CDS             complement(4077..4325)
                     /locus_tag="VG754_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19232.1"
                     /translation="MKTTIELDASNRIVLSRELRRAAGIPRRQKLLAYATPGRIVLEM
                     EPNVSGHVVKRGKLKVWTGAVPATPIEEAVEQSRHYSR"
     gene            4558..>5173
                     /locus_tag="VG754_08195"
     CDS             4558..>5173
                     /locus_tag="VG754_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19233.1"
                     /translation="MPEKTVNDLPRDLRLLYQRGVEALQRDNFDYAIEMFTQVLAREP
                     SVFEIRKTLRTAQMAKSGKSGGFFKRAFSSASSSPQVAKGQMALRRNPLEAMQIAEEI
                     LNSDANSHDGHKLMADAATAAEMPKVAIMSLDILVKAAPKDKELSYQLAEAYALAGEK
                     TKGEEVLADLQREYPTDGEVSQKLKDLSARKTLDEGGYGALAGGG"
BASE COUNT         1163 a         1376 c         1506 g         1128 t
ORIGIN      
        1 tggttgttca ggttgctggt gatgttcgcg aggttttgca aggtgagatt gagcgtcatc
       61 aggttggtgt ccaaattggt gagcgtgccg ccggcggctt gcaaggtgga atcgagcttt
      121 tgattgatgt tggtcggcaa cacccattcg ccgagcgagc cattcgggtc ttttaatcgc
      181 tcggtgatgg cgttgagatt tgcgactgcg ggacgggcat tcgcggcgat ggcattgagg
      241 ttggaagtca aatcagaagt gttggcgaga acgcgatcaa ttttgttggt aaggtccaaa
      301 atgcccggca gcgcggcctt cacctgcaag accatggatt gcaactcgtc gttcaacgcc
      361 ggctcttcat aacgcggcag tccgtagatg ttcgtccctt taaataattc gtagctgcgc
      421 ctgtcatcgt tccaaacagc ggtgatgtat cttttggtga cgttgggatt gatcgcgcaa
      481 aacgtgttgg agccgagctg ttcgctgagt ttattccagt tgttcgtgag cggtttccat
      541 gctccaagaa cgaggttggt gccgtcgcgg atttcctgac cgagccgcca ttcctccgga
      601 tgcggcaacg ctttcgcctc ggcgaccgtc atgtcattgc gaaaaccgta ggagatgtaa
      661 gtcgcataac cgatcaggcc cttgctgatg tccagttggc gagcgttcaa aaaacctgac
      721 ttgatggaga cccgcgacga gtcggtccag atataaccga aattgggttc gagcacttcg
      781 aatttgacgt aaacgttgtt ggtgacaatc tggccccaca tcggcggcat cggtttgatt
      841 tccaaaatgc gtcccacatt aaaacccatc agattgaccg gcccgccgac agccagcccg
      901 tcgccgcgct cggtataaac gaaatactgc gccttgatct tgaaccaacc gcggctttcg
      961 gcggctttgt aaatatagta gccgagaccc acgagcagca gcagcgtcgc aagcatgatg
     1021 aaccagccca cggcgcgttc catgcggttg agccgggtgc gaagttgagg agtgaggtct
     1081 tgcaggggca taggttaacg cgatggagtg atggagtatt ggagtgatgg aaaaatggaa
     1141 atagtggagc gcggttgtgc gcgaagcgtc agccgcagca agccacagaa atactgagag
     1201 ttccacgctg ctgcgactgg tcctgcgtac acagtcgcgg tccattcctt aattatgagc
     1261 tggcctggag agcggtagag tcgcggtttc attgtaattg ctcggcgaga agctcattca
     1321 cgagcggttc ctgatggttg gcgaacttcg ggcgatggcc cagcgggatg aatcgttgct
     1381 tcttgaggat ggcgaaatgg ctgtcgaagt ttttccatgg gcgcaaatct tccgccgtga
     1441 cgatcaccgt gcgcgggcgt tcggcggttt gttgcgcgtg ccaattgcga agaaaattga
     1501 gccaccaatt ggcatggcgg aaatcaagtc caccgagcgg atgatccagc agcaacactt
     1561 ccggttcgag catcaaagcg cgggcgagtc cgccgcgttt ctgccaattg cgcccgagca
     1621 agccgggcat gacgtccgcg taaggcgtga gttcggcggt ttccaacatg gccttcacgc
     1681 gcgcctcggc ctccgtccat tcgatttccc gatgataatg caacggaagc gcgatgtttt
     1741 catgcacgtt gagatggcgc aataattggc cgttctcaaa aaccaagccg agacgcaacc
     1801 gttccgagag ttgctcttcc gaatacatcg gcatttcgtg gccgaacagc cggtacgtgc
     1861 cgttttgagg cggcatgatg ccggcggtca tccacatcaa atcggttttg ccggagttgt
     1921 gcatgcccgc gatgacccaa aactcgcccg cgttcaccgt ccaattcacc tcttccaaag
     1981 tgatttgcgt caggtccatt atggaaccga cagacacgcc ctgcatctcg atgatgggcg
     2041 ctgtgctgga atattttgag gcctcgggtt ccatgatcac gtgacaagat aaacaaaaat
     2101 aaagactgca tccaacgcca cgcacgcgac gatgctttgc gtgactgcgc cgacggttgc
     2161 ctgcgacact tcctccagtc gcaacggttg ggccagccca tgatagcagg tgatgaccgc
     2221 gatcaacacg ccgaacagag acgatttcaa cgcgaggatg acaaagtcca tgccgttcaa
     2281 cgcgtgcgcc agttgttcaa aatattcagc gggcgacaat gggacgtttt gcaaaaacgc
     2341 ccaaagataa ccgctcacca gcgcgcccat gatgaaataa atcgtcagcg aaaacattcc
     2401 caacgccatg ccgatgacac gcggcatgac aaaataatgg acgggatcga tgcccaacgc
     2461 ttcgagcgcc tccacttcgc ccaacgcgcg cgcggtgctg agttcgatga cgttggcggt
     2521 gccggaacgc gacagcacga gcatggcggt gatgagcggg ccgagttcgc gcacgaccac
     2581 cgcgaccatg atggtgccga tgacattggt gctgccgact tggtaatgaa acaaaagcgt
     2641 gagcgtctgg ccgatgacca gcaagccgag cacgcccgac atgaacagga acatcgggat
     2701 cagccgcagg ccggaacgcc gcaattcccg gaaaatggcg ggccacgtga cgctttccgc
     2761 cgtggtcaat ttcgtgagga tgactccgag cgtgatgagc gcgaaagcgc cgaggccatg
     2821 gacggtgatg agcaggcgga taagttgccg gccgaaataa ttgctgtacg ccttcggcaa
     2881 cggcgcgcgc atcaaggcgc gcgcccgcga tgcgtaaagc gcactgaatc ttccactgct
     2941 cacgcggcaa acgtatgttc tccgcaacgg acggtcaaag gggtttttcc ttctgcccgg
     3001 acattcaaca tcgaatgccg aacgccgatg taaaaagtaa tcatcctcaa caattgactt
     3061 tcacctcctt tatgaatttt aattctccgt ccgccgaatg aagaacgttg tttatttcga
     3121 tcttgaaacg cagaaatccg ccgatgaggt gggcggctgg gacaagatca gcgccatgcg
     3181 catgagcgtg ggcgtcacct acagcacgac gcgcggcgat tacaggattt acggcgagaa
     3241 acaggtggac gacctgctca ctgaattgca gcgcgcggac ctggtggtcg gattcaacca
     3301 tctgcggttc gattacgaag tgttgcacgg ttatacctcg ctcgatctgc ggcagcttcc
     3361 gacgctcgac atgctggtgg aattgcaaac cacgcttcaa catcgtctct cgctcgattc
     3421 catcgcgacg gccacgttcg gcgtggaaaa aaccgccgag ggtttgcagg ccatccaatg
     3481 gttccgggaa ggccggttga tggacgtcgc agaatattgc tgttatgacg tgaagctcac
     3541 caagctcgtt tacgaatacg gcgcgaacca ccggcaactc cattaccaca ataaattcgg
     3601 gaaaaaattg accgtcccgg tgaagtggtg aatgtttttt taaccacggg tggacacagg
     3661 taaaggaata aagccatctt aaggaatcac gatttcgata tccggcgcca catgctgaaa
     3721 atttgacggg ttgcgtgtca caatccgagc cgcctttttg cggcgggcaa atgtcgcgtg
     3781 cagagaatcg taaattcccc cgcccatgac tccccgactg cgcgcctcgc gaatcgcggt
     3841 ttcgtaatcc gccaatgcca gcgcctccac aacgaccacg ccgcgcaaac ttaaagtcaa
     3901 ctcagccgcg gcgtccgtcg ggaccttgta aaagccggtg agtgtggcga aggtttccgc
     3961 cattgcgtgg gcgtccgtga acggctgcgc ggaatcttca agcgccgcga tgcatgcctc
     4021 gtgttctgga tgcgacttca agagcgcccc gaccattatc ccggtgtcca gaaaggtcat
     4081 cggctgtagt gacgtgattg ttcaacagcc tcctcgatcg gcgtggccgg gaccgcccct
     4141 gtccagacct tgagtttgcc ccttttaacg acgtggccgg aaacattggg ttccatttcc
     4201 aaaacgatgc ggcctggcgt cgcataggcc aacagttttt gtcgtcgggg aatcccggca
     4261 gcccgccgca gttcccgcga aaggacaatc cggttggagg catcgagttc aattgtcgtt
     4321 ttcatgccat aagtatggcg tgttcatggc gtgttgacaa gctcccgata tgatactcaa
     4381 tcctgccata ttttactcgc ttcatcgcga aaagtgatgc gaacttcccg gcattttcca
     4441 aattcatgag aattcgcgcc atttgtccgt ttcatctgcg ttcatctgtg tcgatccgtg
     4501 attgatccgt ggttgattta atctggattt ttttgggagt tctggcacaa tttgtgtatg
     4561 ccagagaaaa cagtcaatga tttgccgcgc gacctgcggc tcctttatca aaggggggtc
     4621 gaggcgctcc aacgggacaa tttcgattac gccatcgaga tgttcacgca ggtccttgcg
     4681 cgcgaaccat cagtgttcga aatccgcaaa actttgcgca ccgcgcagat ggccaagagc
     4741 ggaaaaagcg gcggtttctt caaacgcgcg ttcagcagcg caagttcttc tccgcaagtt
     4801 gccaaggggc agatggcgtt gcgcaggaat ccgttggaag cgatgcagat cgccgaggaa
     4861 atcctcaatt cggacgcgaa cagccatgac gggcataagt tgatggcgga cgcggcgacg
     4921 gctgccgaga tgccgaaggt ggcgatcatg tcgctggata ttcttgtgaa ggccgcgccc
     4981 aaggacaagg agttgtcgta tcagcttgcg gaggcttacg cgttggcggg cgagaagacg
     5041 aagggcgaag aggtgctggc ggatttgcag cgcgaatatc cgaccgacgg ggaggtttcg
     5101 caaaagctca aggacctttc ggcgcgcaaa acgctggatg aaggcggtta cggggcgttg
     5161 gcgggcggcg gag
//
LOCUS       DASZMM010000338         5105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2339413, whole genome shotgun sequence.
ACCESSION   DASZMM010000338 DASZMM010000000
VERSION     DASZMM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339413"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VG754_08200"
     CDS             complement(<1..200)
                     /locus_tag="VG754_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HWF19234.1"
                     /translation="MPHKKNFKAEILSTHTAALFKTAVKRAAELLRAGEVVALPTETV
                     YGLAANALNPEAVARIYEIKGRP"
     gene            complement(260..679)
                     /locus_tag="VG754_08205"
     CDS             complement(260..679)
                     /locus_tag="VG754_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19235.1"
                     /translation="MTEPMRWRMFAYMAALFIAGVISGAAVMSRNPIPQTLKVGRLEE
                     IASMIRQKLIINLELTPEQRDKFEPLIKKTAEEMEASHVDCLKRIDDAVNKMHAQILP
                     DLTPEQREKMKQVEVERRELMRKKYNYPPDTAKAGNP"
     gene            complement(676..969)
                     /locus_tag="VG754_08210"
     CDS             complement(676..969)
                     /locus_tag="VG754_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19236.1"
                     /translation="MNPEDEKLNRLLKSAAAAPRPPSGAAAFVLEARVLAHWRASQAD
                     DDGAFLVAWFRRAAICGCVLAVASLAWTQVQRTDVGGGAMAVADSAMQMGVEP"
     gene            complement(974..1552)
                     /locus_tag="VG754_08215"
     CDS             complement(974..1552)
                     /locus_tag="VG754_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF19237.1"
                     /translation="MSESGFDMSACLERIRNRDEDAARQLFHHLYPLVIKVVRSHLPR
                     RMDEEDLIQTVYMKVFANLNQYSGLAPLEHWVSRIAVNTCIKALRAEKARPELRWADL
                     AEDETDVLDWLASTDEDVRPDRHLASRELVEKLLGQLKPKDRLVITLMTLEGRSIEEV
                     REITGWNQSVIKVRAFRARAKLRKLLKTLTKE"
     gene            complement(1944..3146)
                     /locus_tag="VG754_08220"
     CDS             complement(1944..3146)
                     /locus_tag="VG754_08220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWF19238.1"
                     /translation="MLEGVKAMGYVEPTPIQLRAIPLILAGQDVIGSAQTGTGKTAAF
                     ALPILSKLGEHSRTPRVLILEPTRELAAQVETAIRDFARFTKLRAAVMYGGVGYGRQM
                     DALRDGVDIIIATPGRLLDHLNRGTCRLDHVQYLVLDEADRMLDMGFLPDVRRIVERC
                     PRNRHTSLFSATIPVQIETLIKWAMRNPQTIEIGVRRSPAETVKHVIYPVAEDQKSDL
                     LRELLERVNYESVIVFCRTKHRADRIAHLLKRNNHAVAVLHSNRTQREREDALKGFRN
                     GRFEVLVATDIAARGLDIADVSHVINYDVPQHPEDYIHRIGRTGRAENTGDAFTLMVA
                     EDAQHVHSIERFIGKKVERIKLENFNYKYTALFEEGKPGQQQGYPRKAKGVRLSGGYY
                     FGPAKRRR"
     gene            complement(3296..5065)
                     /locus_tag="VG754_08225"
     CDS             complement(3296..5065)
                     /locus_tag="VG754_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19239.1"
                     /translation="MSTSPEAEFDLEKLFLPAWAQESPAVNRYAKYEGDNRPVDRDRK
                     FDDRRAGGRRPPRRDGPRPGGGPGGPNRDQNRQPRNDRPAGPGGPRREGDRGQRPGGP
                     GGPRRDFRRDDRDRGGPREPAIPLPEISITLMPDDKGVDSLSRQIKMSGRSFPLFDIA
                     QMILQKPERQQVRFDVKKKPDGTIAQPLFLCAVDDTLWLSEQEAIEHLLSKHFGMFYQ
                     AEKTPTEPPKGTYTFVAQCGMSGIILGPPNYHDYQNQLHKLHTERFSRMPFDMFKARV
                     KIVREEAVVKKWIEDQSVKTEYVCLNVPEATRLGSREAVEAHFREVHLANLIKQVEKH
                     TLSGAASRQLRSPGLQRLLRIAWDEQKRFPLQIATILSQQFASHGLQFFKVNKTITHV
                     SVARPHYLDLEATPVSNQVKEIVQFINAHPRCTRRLLLEKLAPTPPIPVHPPTPVAAE
                     AAAPASTDGTPAPAAPVQPAAPAEPTTTPEQAAIIGDLHWLIHQGHVIEFANGILETA
                     KKPVVKPPKAPKPAPAEQAAAVAEQTVTAPEAVPVEAAAPPVEVAAAPDEAAEPSMAT
                     ETTPPVSVESAPVSEPPHEPATT"
BASE COUNT         1015 a         1455 c         1458 g         1177 t
ORIGIN      
        1 gggcgtcctt tgatttcgta aatccgcgcg accgcctccg gattgagcgc gttggccgca
       61 aggccataaa cggtttccgt tggcaatgcc acgacttcgc cggcgcgcaa caattcggcg
      121 gcgcgtttca ccgcggtttt aaaaagcgcg gcagtgtgag tggacagaat ttccgccttg
      181 aaattttttt tgtgcggcat caactcaacc cggctgatca acaacttgct catgcagtct
      241 cagacacgaa cttcccgact caaggattgc cggccttggc cgtgtccggc ggataattat
      301 attttttgcg catcaactca cgccgctcca cttcgacttg tttcatcttt tcccgctgtt
      361 cgggggtcaa gtcgggcagt atctgcgcgt gcatcttgtt taccgcatcg tcaatgcgtt
      421 tgaggcagtc cacatggctg gcttccatct cttcggccgt ttttttgatg agcggctcaa
      481 acttgtcccg ctgttccggc gtgagttcca agttgatgat gagcttttgc cggatcatcg
      541 acgcgatctc ctctaaacgg cccaccttca aagtctgcgg gatgggattg cgcgacatca
      601 ctgccgcgcc gctgatgacg cctgcaatga acaacgccgc catgtaggcg aacatgcgcc
      661 atcgcatcgg ttcggtcatg gctcgacccc catctgcatc gcggaatcag ccacagccat
      721 ggcgccgccg ccgacatccg tccgctgcac ctgggtccaa gccaggctcg ccacagccag
      781 cacgcaaccg cagatggccg cgcgccggaa ccatgccacc aggaatgctc cgtcgtcgtc
      841 cgcctgtgaa gcgcgccaat gggcaagcac acgcgcttcg agcacgaatg ctgccgcgcc
      901 cgacggcggc ctgggtgcgg ctgcggctga tttcaacaac cgattcaatt tttcatcctc
      961 tgggttcata actttactct ttcgtcaatg tcttgagcag cttgcgcaat ttggccctcg
     1021 cccgaaaagc ccggaccttg attactgact ggttccaacc cgtgatttcc cgcacttcct
     1081 cgattgaacg gccttccagc gtcatcaagg tgatgaccag ccggtccttc ggtttgagct
     1141 gcccgagcaa tttctccacc aactcccgcg aggcgagatg gcgatccgga cgcacgtctt
     1201 catccgtgct cgcaagccag tccagaacgt cagtttcgtc ctccgctaga tcggcccatc
     1261 gcaactcggg ccgcgccttt tccgcgcgca acgccttgat gcaggtattg acggcgatgc
     1321 gggaaaccca atgctccagc ggggcaaggc cggaatactg attcagattt gcaaacactt
     1381 tcatatagac cgtctggatg agatcttcct catccatccg ccggggaaga tgggagcgaa
     1441 ccactttgat caccaacgga tacagatggt gaaacaactg gcgcgcggca tcctcatccc
     1501 gatttcggat ccgctccaga caggccgaca tgtcgaaacc cgactcagac ataagtctag
     1561 tttgttccaa tgtcggcctt actgcatcat ttatttttaa gacctgttgg cggaggaaaa
     1621 gttacatatt ccgctgcaat aacgtgcttt ccatccgccg gtaaaccgat ttgtaatagg
     1681 tcagatttaa gggaattaac cttgaaaatc agcataaagg ccgcaatccg ctctgaacca
     1741 atgctttgca cagccagctc ttttggcgca ttggtttatc aaatacgccg gtaagggttt
     1801 atctgacagg aaaatcagaa accggaggac aggcaatatt ttccaatccg cgtaagctga
     1861 ttacggttga tagggtagtt gaagtattgt ggttggaccg gcatcgagtg atgccggtct
     1921 ttttttatgg gctgtcaaat tggctatctc ctccgctttg cagggccgaa ataataaccg
     1981 ccgcttaaac gcacgccttt ggccttgcgc ggatagcctt gctgctggcc gggtttgcct
     2041 tcttcaaaaa gcgcggtgta cttgtaattg aaattctcca gtttgatgcg ttcgactttc
     2101 ttgccgatga atcgttcgat cgagtggaca tgctgggcgt cctcggcaac catcagtgtg
     2161 aaagcgtcgc cggtgttttc agcgcggcca gtgcgcccga tgcgatggat ataatcttcg
     2221 ggatgttgcg gcacgtcgta attgatgacg tggctgacgt ccgcgatgtc caatccgcgc
     2281 gcggcgatgt ccgtggcgac cagcacttcg aaacgtccat tgcgaaaacc tttcaacgca
     2341 tcttcgcgct cgcgttgcgt gcgattggaa tgcaacacgg caacggcgtg attgttgcgc
     2401 ttgagcaggt gcgcgatgcg gtcggcgcga tgcttggtgc ggcaaaaaac aatgaccgat
     2461 tcgtaattca cccgttcgag caattcgcgc aggagatcgc tcttctggtc ttcggccact
     2521 gggtaaatga cgtgtttgac cgtctcggcg ggcgagcggc gcacaccgat ctcgatggtc
     2581 tgcggattgc gcatcgccca tttgattagg gtctcgatct ggacgggaat ggtcgcggag
     2641 aaaagcgatg tgtgccggtt gcgcggacaa cgttccacga tgcgccgcac gtccggcaga
     2701 aaacccatgt cgagcatccg gtccgcttca tcgagcacga gatattggac gtgatcgagc
     2761 cggcaggtgc cgcggttcaa atggtcgagc agccgtcccg gcgtggcaat gatgatgtcc
     2821 acaccgtcgc gcaacgcatc catttgccgg ccatatccta cgccgccata catcaccgcg
     2881 gcgcgcagtt ttgtgaagcg ggcgaaatcg cggattgctg tttcgacctg ggcggcgagt
     2941 tcgcgcgtcg gttcaaggat taaaacacgc ggcgtccggc tgtgttcgcc gagtttgctg
     3001 aggattggca gggcgaacgc agcggttttg ccggtgcccg tctgggcgct cccgatgaca
     3061 tcttgtccgg cgagaatgag cggaatggcg cgaagctgaa tcggcgttgg ctcgacataa
     3121 cccattgcct tgactccctc gagcaaggca tctgaaagac ccaatttact aaatggcata
     3181 actgttaaac gcggaatgcg gagtgcggaa tgcggagttg ccgaaaagtc gctctcccga
     3241 tatggaaatc attattaaaa tattattcat taaacaccct tgtgcaaacc ctgaatcatg
     3301 ttgtcgccgg ctcatgtgga ggttcggaaa cgggcgcgga ttccacggaa acaggcggcg
     3361 tcgtttcagt cgccatgctt ggttctgctg cctcatcagg cgctgctgca acctccactg
     3421 gtggcgcggc ggcttcaacc ggaacagctt caggtgctgt gacagtttgc tcggcaacag
     3481 cggcagcctg ttccgccggg gcgggttttg gcgctttggg cggcttgacg acaggttttt
     3541 tggcggtttc caaaattccg ttcgcaaatt caatgacatg tccctgatgg atcaaccaat
     3601 gcagatcgcc gatgatggcg gcttgctccg gtgtcgtagt tggctcggcg ggcgcagcag
     3661 gttgtactgg cgcagccggg gcaggagtgc cgtccgttga cgcgggcgcg gcagcttccg
     3721 cagcaacagg tgtcggcgga tgcaccggaa tcggcggcgt tggcgcaagt ttttcgagca
     3781 acaagcggcg agtgcatctc ggatgggcgt tgatgaattg cacgatttcc ttgacctggt
     3841 tcgaaaccgg cgtggcttcg agatcgaggt agtgcggccg cgcgacagag acgtgcgtga
     3901 tggtcttgtt gaccttgaaa aattgcaggc cgtgactcgc gaactgctgg ctcaggatgg
     3961 tcgcgatctg caacggaaaa cgtttctgct cgtcccaggc gatgcgcaac aggcgttgca
     4021 atccgggcga acgcaattgc cggcttgccg cgccgctcaa ggtatgtttc tcgacctgct
     4081 tgatcagatt ggcgagatgc acttcacgga aatgcgcctc gacggcttcg cggctgccca
     4141 agcgcgtcgc ttcgggcacg ttcaggcaga cgtattcggt cttgacgctc tggtcttcga
     4201 tccatttttt caccaccgct tcctcgcgga caattttcac tcgcgctttg aacatatcaa
     4261 acggcatccg ggaaaaccgt tccgtatgca gcttgtgcaa ttgattttgg taatcgtgat
     4321 aattcggcgg gccaaggatt atcccgctca tgccgcattg cgccacgaac gtgtaagtgc
     4381 ccttgggcgg ttcggtggga gttttttccg cctgataaaa catgccgaaa tgtttgctca
     4441 acagatgttc aatcgcctcc tgctcggaga gccaaagcgt gtcgtcaacg gcacacagga
     4501 acaacggttg cgcaatcgtg ccgtccggct ttttcttcac gtcgaaacgc acctgctggc
     4561 gttccggctt ctgcaaaatc atctgcgcga tgtcgaacaa cgggaacgaa cgcccgctca
     4621 tcttgatctg gcgcgaaagt gaatccacgc ccttgtcgtc cggcatcagc gtgatgctga
     4681 tttccggcaa tggaattgcc ggttcacggg gaccaccgcg atcgcggtcg tcgcgtcgaa
     4741 aatcccttct tggaccgccg ggaccgcccg gacgttgtcc acggtcgcct tcacggcgcg
     4801 ggccgcccgg ccctgccgga cggtcattgc gcggctgacg gttttggtca cgattgggac
     4861 caccaggtcc gccaccggga cgcgggccat cgcggcgcgg aggacggcgc ccgcctgcac
     4921 ggcggtcgtc aaacttgcgg tcacggtcaa cggggcggtt gtcgccctcg tatttggcgt
     4981 aacgattgac cgcaggcgat tcctgcgccc acgcggggag gaaaagcttt tccaaatcaa
     5041 attctgcttc cggagatgta ctcattcagt cagttgcaaa aagcaaaggg cctttgcgcc
     5101 ccaaa
//
LOCUS       DASZMM010000339         6261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1520045, whole genome shotgun sequence.
ACCESSION   DASZMM010000339 DASZMM010000000
VERSION     DASZMM010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520045"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="VG754_08230"
     CDS             complement(<1..1753)
                     /locus_tag="VG754_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19240.1"
                     /translation="MFGLLSYVLTATAAEPFQVVTGPYAAPIPSAGAGDSWSPVVSQD
                     GRYVLFASSANNLTLTSSNSSFVPVFPAVDNVFLRDRTNGTTMLVSVNLAGTGGGSGG
                     DSFPVDISTNGQYVLFESSATNLVPGVNNHFNNVFIRDVVNGLTSLVTVNTNGMSGNG
                     ASHSSAMTSDGRYVAFVSAASDLVPGDSNKIEDVFVRDMQLGNTVLASPGAMGTAPYG
                     SDWPAITPDGHYVVFYSTATNLVPGITNRSEIYIRDLVAGTTTLASAFSHTVLPSVFN
                     ITNGVSFNHVVSADGQYVAYQAGKSASNGAPGIILRYNAFTGFTDTIATNAAAGRLGA
                     ETNYHSLDMSADGRFVCFVTNLSTSGSNSAINVWDAQSNTFTLVSGNLTSNAVPTNTL
                     CDWPVMDSTGQYIAFLSSATGLTSNSVSAGYHLYFRDIQPGVTQLLDVDTNGVGSISN
                     SLTFPRMSKNAHVAAWEAPDGSLVAGDNNQAYDVFARDIVNQDTELISIRRNELPCLT
                     PGGRCLMLSSSVSTNARYIAYSCDANNLTMGVTNGFFNVYARDLLTDANALVSVDRNG
                     VLPGNGLSVDATISADGR"
     gene            complement(1963..2856)
                     /locus_tag="VG754_08235"
     CDS             complement(1963..2856)
                     /locus_tag="VG754_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF19241.1"
                     /translation="MDTAGANHSRTREKRAFTLTELLVVLVVLAIFAALSIQAMTKSK
                     AKTIQCLNNHRQLMSAALIYADEFSGIWIPNEPEGTGGQTDWVSLDLDWNAGRSDNTN
                     YSKLTNPQYSLLAPYLGGSYSIFHCPSDQSFVTQEGPRVRSVSANQAVGSVWVTTECC
                     ISNGPVNGQWLTGANIGGGCQTVWRCYGKTTDFVAPGPSLTFVFADEHPNSINDSQLA
                     FQCAQQGIGAPFIDRPANYHNAAGTFSFADGHVEIHKWVGPSLGTGPDFWTGPNYSLN
                     VTSQADNQDLMWIQQRTSAHK"
     gene            complement(2925..4073)
                     /locus_tag="VG754_08240"
     CDS             complement(2925..4073)
                     /locus_tag="VG754_08240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF19242.1"
                     /translation="MALMTDEPPATSKIIFVTHEFAPFRGGVATFVEEVGRTSQRKGA
                     SVEIWAPDYGLPQSTQMDCPVVRLRAEGSLKWKHLTQLTRALAERRAELEGATVVMAS
                     VGAHIAFMMLAISGRVKMKSLVSVLYGSEVLRFGRNPIWRLLAWRLYRRVEGIVTISE
                     FSRNLIEHSFLSSFRGTIEIAPCACSSDAMRNVTAKKIEDGKVRIFTLARIHPRKGQL
                     DTALALGRLPAELRSRVVYQIGGTGDSSYLKKISQTCAQAGVAFEYLGEVKPESLADA
                     YAQCDIFAMTSRRLPRSVEGFGITYLDAAFHGNPVVAFKSGGAEEAVRDGETGLLAEE
                     GDLAAVTDIFARLIADAGLRERLGAAGRERARQQTWATAAAVFLRFRG"
     gene            4182..5057
                     /locus_tag="VG754_08245"
     CDS             4182..5057
                     /locus_tag="VG754_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWF19243.1"
                     /translation="MNATLSDSDCADLKEGLRRCSPETIAAAICYRETGDLASIPKVV
                     MGILERYQPASAPVKLSEARDNMRLIEELGLDSLTLLEIVMSIEEVLKIRIENEALRE
                     IRTLGQLNDFLHAKITGVATSAAGRHYNREQIILILPQQPPFLFLDSAELEESSVKGH
                     YQIKGDEFFLEGHFKDDPIFPASIVFEAMGQTACLWVLEKAPARTGKQIASNQVYFAS
                     LDGAHFQRKTRPGERLEFELKLTKLREPLAVFEGVVTVNGESVARIERLVLAFGETLQ
                     SEKPVELAASRNGHH"
     gene            5057..>6261
                     /locus_tag="VG754_08250"
     CDS             5057..>6261
                     /locus_tag="VG754_08250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWF19244.1"
                     /translation="MKRVVITGLGFITSIGNDQNTVLRNLRECRTGVELFPEFAGADI
                     PVKLAGTVKGFQFPTAYFEDWTYPPEYKLTREQLRPMAPNSLYAYCAMKQAVEDARLT
                     PEIVSRPETGMMSASGGSMWLAHENLQTMLTRGVNRCQPMGIINSIPGSLYINLAALF
                     KIKGATLGFSSACSSSAHAIGAAFDLIRLGRQQVVFAVGAEDCNKISILPFASVRALT
                     VQTDPERSPCAFDIKRDGFAATGGAAVLVLEELEHARRREAPIYAEILGWAQTSDGYN
                     VLAPDPQGDGLRRAMEAALTDANIRREDVDYINAHAPSTPFGDASEIQAIKNVFSGSR
                     VPFVSSTKSLTGHGLSLAGAMETAFCCLALKEKFTPVSAHITQLDSMCDGVPVVTKTI
                     AEYPRIALK"
BASE COUNT         1427 a         1803 c         1624 g         1407 t
ORIGIN      
        1 atcggccatc ggcgctgatg gtcgcgtcaa cggaaagccc attgcccgga agcaccccgt
       61 tcctgtccac actgacgaga gcgttggcgt ccgtcaacag atcgcgtgca tacacattaa
      121 aaaatccatt tgtgacaccc attgtcaggt tgttcgcatc acacgaatac gcgatgtaac
      181 gcgcgtttgt gctgactgat gaactcaaca tcaggcagcg tccgccgggt gtcaggcagg
      241 gtagttcgtt gcggcgaatc gaaattagtt ccgtatcctg attgacgata tcgcgcgcga
      301 acacgtcata agcttggttg ttgtcgcctg caaccaggct gccatccggc gcttcccacg
      361 ccgcgacatg cgcgtttttg ctcattctcg gaaacgtcaa tgaattcgag attgatccga
      421 ctccatttgt atccacatcc agcaactgag tgactccggg ctgaatatcg cgaaaataaa
      481 ggtgataacc tgcgcttacc gaattgctgg tcaatcccgt ggcgctgctt aaaaaggcga
      541 tgtattgccc ggtcgaatcc atgacgggcc aatcgcaaag cgtatttgtc ggcacggcat
      601 tactcgtcag atttccgctg accagcgtga aagtattgct ttgcgcatcc caaacgttga
      661 tggcgctgtt gcttcccgaa gtgctgagat tggtgacaaa gcaaacgaag cgtccgtccg
      721 cgctcatgtc cagcgaatga tagtttgttt ccgcgcccaa cctccctgca gccgcattcg
      781 ttgcgatggt atcggtgaat ccggtgaacg cattgtaacg caaaatgatt cctggcgctc
      841 cattgctggc gctcttgccg gcctgataag ccacatattg tccatcagcg ctcacgacgt
      901 gattgaatga gacgccgttg gtgatgttaa acaccgacgg caatactgta tgggaaaatg
      961 cgctggcgag agtggtcgtt cccgcgacca agtcccggat gtaaatttcg gacctgtttg
     1021 ttattcccgg aacgagattg gttgctgtgc tatagaaaac tacgtaatgg ccgtcgggag
     1081 ttatcgcagg ccaatcggaa ccatacgggg cagttcccat ggcacccggg ctggcgagca
     1141 cagtattgcc aagctgcatg tcgcggacaa aaacatcttc tattttgttg gagtcgccgg
     1201 gcaccagatc gctggcagcg ctgacgaatg caacataacg cccgtccgaa gtcatggcag
     1261 agctgtgcga agctccattt ccggacatgc cgttggtatt gacggtcacc aagctcgtta
     1321 agccattgac cacgtcgcgg atgaagacat tattgaaatg attgtttact cctggcacaa
     1381 ggttggtcgc gctgctttcg aacaggacgt attggccgtt cgtggaaata tccactggaa
     1441 aggaatcccc gccgctgccg ccgcccgtac ccgcgagatt aacactgacc aacatggtgg
     1501 ttccattggt gcggtcgcgc agaaatacat tatcgacagc cggaaacacc ggcacaaaag
     1561 agctgttgct gctggtcaag gtgagattgt tcgccgaact cgcaaacaac acataacgac
     1621 cgtcctgact gaccaccggg gaccatgaat ctccggcgcc cgcgcttggg attggtgcgg
     1681 cgtagggacc agtaacgact tgaaaaggct cggctgccgt ggcggtcagc acgtaactta
     1741 gcaatccgaa caaggctcga agcgcgcagt gtagaatacg ctgatttgtt tttgtggtta
     1801 atcgcgtcat taggacctga actatgaacc tcattggtct cgttaaatct ggctttggac
     1861 ggctataact atatgcagtg tgccctttta tgtcaacgcg aatcaaagca gaaatttggg
     1921 gggtgggacg cacttttgtt cttttctagg cgcgctggat aattatttgt gggccgaagt
     1981 gcgctgctgg atccacatca agtcctggtt gtccgcctga ctggtaacgt tcaggctgta
     2041 attgggacca gtccaaaaat caggcccggt cccaagagaa ggcccaaccc atttatgaat
     2101 ttcgacgtgg ccgtcagcga aggaaaaagt cccggcggca ttgtgataat tggccggacg
     2161 atcaataaat ggcgcgccta tcccttgttg cgcacattga aaggccaatt gagaatcatt
     2221 gatgctgttt ggatgctcat cggcgaatac aaatgtcaat gaaggtcccg gcgcaacaaa
     2281 atcagtggtt ttgccgtagc aacgccagac tgtctggcaa ccaccgccaa tgtttgcccc
     2341 ggtcagccat tggccattca ccgggccatt ggatatgcag cattccgttg tgacccaaac
     2401 cgaaccaacc gcctgattcg ccgaaacgct tcgaacgcgc ggtccttcct gagtcacaaa
     2461 actctgatcg ctcggacaat gaaagattga gtagctgcca ccgagatatg gcgccaggag
     2521 ggaatattga ggattggtca gcttgctgta gttggtattg tcagaacgac cagcattcca
     2581 gtctaagtcg agacttaccc aatcggtttg tcctccggtg ccctccggct cattgggaat
     2641 ccatatcccg ctgaattcat ccgcgtaaat cagtgccgcc gacataagct gtcgatgatt
     2701 gttgaggcat tggatggttt tggccttgga tttcgtcatc gcttgaattg aaagggccgc
     2761 aaaaatcgca aggacgacga ggacaaccaa cagttcagtc aatgtgaacg cccgtttttc
     2821 acgggtgcgg gaatgatttg ctccagccgt atccatggta gagtttttat tgccttgttc
     2881 gcgtataaat gcaagttttt tgttcctcgc gaatcaaagc tgactcaccc ccggaatctt
     2941 aaaaataccg ccgctgcggt ggcccaagtc tgttggcgtg ctcgttcacg tccggcagcg
     3001 ccaagacgtt cgcggagacc ggcatcagcg attaaccgcg cgaagatgtc cgtcaccgcg
     3061 gccaaatcgc cttcttccgc aagcaatccc gtttcaccgt cccgcaccgc ctcctccgca
     3121 ccgccgcttt taaatgccac cacggggttt ccgtgaaatg cggcatccag ataagtgatg
     3181 ccaaagccct ccacactgcg cggcaagcgg cgactggtca tcgcaaaaat atcgcattgc
     3241 gcataagcat cggccaacga ttccggcttc acttcgccga gatattcaaa agcaacgcca
     3301 gcctgggcgc acgtttgcga aatttttttc aaataactcg aatcgcccgt tccgccgatt
     3361 tgataaacca cccgtgatct caattccgcc ggcaaccggc ccaatgccag cgccgtgtcg
     3421 agctggcctt ttcgcggatg aatccgggcc aatgtgaaaa ttcgcacttt tccatcttcg
     3481 atttttttcg cggtgacatt tcgcatcgca tcgctgctgc aagcgcaagg cgcaatctcg
     3541 atggtcccgc ggaatgagga caaaaaactg tgctcgatca aatttctcga aaattccgat
     3601 atggtcacaa tcccctcgac gcgccgatag agccgccacg caagcaagcg ccagatggga
     3661 tttcgtccaa agcgcagcac ttccgaaccg tataacactg aaaccaacga cttcatcttc
     3721 acgcgtccgg agattgccag catcatgaac gcgatatgcg cgccgacgct tgccatcacc
     3781 acggttgcgc cttccaattc cgcccgacgc tcggccagcg ctcgcgtcaa ttgcgtcaaa
     3841 tgcttccatt tcaaacttcc ctctgcccgc aaacgaacaa ccggacaatc catttgggtg
     3901 ctctgcggaa gtccgtaatc cggcgcccaa atctccactg acgccccttt ccgttgagat
     3961 gtccgtccca cctcctccac gaaggtcgcc actcctccgc gaaaaggggc gaattcatgg
     4021 gttacgaaga taatcttgga tgttgccgga ggctcgtccg tcatcaaggc catcacaaca
     4081 gagccgattc cccaagccaa accaaaaaag cttttaagtg atggcccggt ctggtaatca
     4141 gacggtttaa ctgctacggt gagccgagtg ctcacaggtg aatgaacgcg actttgtcag
     4201 acagtgattg tgccgatttg aaagagggac tgcgccgttg cagtcccgaa accattgcgg
     4261 cggccatttg ttatcgggaa acaggcgacc tcgcctccat tcccaaagtg gtgatgggca
     4321 ttttggagcg ttaccaaccg gcttccgcgc ccgtgaaact ctccgaagcc cgggacaaca
     4381 tgaggctcat cgaggagctt gggcttgatt ccctcacgct tctggaaatc gtgatgtcca
     4441 tcgaggaagt tttgaagatc cgcattgaga atgaagcctt gcgcgaaatc cgcacccttg
     4501 gccagcttaa cgatttcctt cacgcaaaaa taaccggtgt ggccacgtct gccgccggac
     4561 ggcattacaa ccgcgaacaa atcattctca tcctgccgca gcagccgccg ttcttgttcc
     4621 tcgattcggc cgaattggag gagtcctccg tgaaaggaca ttatcagatc aaaggcgatg
     4681 aattttttct cgaaggccat ttcaaggacg accccatttt cccggcttcg attgtattcg
     4741 aagccatggg acaaacggcc tgcctttggg ttctggaaaa agcgcccgcg cggacgggca
     4801 aacaaatcgc ttccaaccag gtttatttcg cttcgctcga cggcgcgcat tttcaacgaa
     4861 aaacacgtcc cggcgaacga cttgagtttg aactgaagct gaccaagctg cgcgaacctt
     4921 tggctgtttt cgagggagtc gtcaccgtca acggcgaaag tgtcgcgcgc atcgaacgcc
     4981 tcgtcctcgc cttcggcgaa actttgcagt cggaaaaacc tgtcgagctt gccgcctccc
     5041 gcaatggtca tcattgatga aacgcgtcgt catcaccggc ttgggcttca tcaccagcat
     5101 cggcaacgat caaaacactg tcctgcgcaa cctgcgcgaa tgccgcactg gcgttgaact
     5161 ttttcctgaa ttcgccggcg cggacattcc cgtgaaactt gccgggacgg tgaaaggttt
     5221 ccagtttccc accgcgtatt ttgaagattg gacttacccg cccgaataca aattgacccg
     5281 cgaacaattg cggcccatgg cgcctaattc gctctacgcg tactgcgcga tgaagcaggc
     5341 cgtcgaggat gcccggctga ctccggaaat cgtctcacgg ccggaaaccg gcatgatgtc
     5401 cgcttcgggc ggttcgatgt ggctcgcgca cgaaaatctc cagaccatgc tcacgcgcgg
     5461 cgtgaaccgt tgccagccga tgggcatcat caacagcatt cccggctcgc tttacatcaa
     5521 tcttgccgcg cttttcaaga tcaagggcgc aacgcttggt ttttccagcg cttgcagttc
     5581 atcggcccat gccatcggcg cggcattcga cctcatccgg cttggtcgtc agcaagtggt
     5641 cttcgccgtc ggcgcggagg actgcaacaa aatctccatt ctccccttcg caagcgtccg
     5701 cgcgctgacc gtccagacag acccggaacg cagtccgtgc gccttcgaca tcaaacgcga
     5761 cggcttcgcc gcgactggcg gcgcggcagt cctcgtcttg gaagaattgg aacacgcccg
     5821 ccggcgcgag gccccgattt acgccgaaat cctcggctgg gcgcaaactt ccgacggtta
     5881 caacgtcctt gcgccagacc ctcaaggcga tggcctgcgc cgcgcgatgg aagctgcgtt
     5941 gactgatgcg aacattcggc gcgaagatgt ggattatatt aatgcccacg cgccttcgac
     6001 tcccttcggc gatgccagcg aaatccaggc catcaaaaat gtttttagcg gcagccgcgt
     6061 tccgttcgtc agcagcacca aaagcctgac cgggcacggc ttgtcgcttg ccggtgcgat
     6121 ggaaaccgcg ttttgctgcc tcgcgctcaa ggaaaaattc actcctgttt cagcccacat
     6181 cacgcaattg gattcgatgt gcgacggcgt ccccgtcgtg acaaaaacga tcgccgaata
     6241 cccgcgcatc gcgctcaaaa a
//
LOCUS       DASZMM010000340         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_710175, whole genome shotgun sequence.
ACCESSION   DASZMM010000340 DASZMM010000000
VERSION     DASZMM010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710175"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1957)
                     /locus_tag="VG754_08255"
     CDS             complement(404..1957)
                     /locus_tag="VG754_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF19245.1"
                     /translation="MSRPIRVLLVEDSELDAALLLLELRQNGYQPECQRVETAAEMTA
                     ALDRQSWDVVIADYVMPRFSGPAALKLMQRRELDLPFIIVSGHIDEDTAVASMKAGAH
                     DYVMKDRLTRLVPAIEREMQEAANRAERREIEKEAAAELAFRHAIEASIPSGIAATDV
                     QGALTYVNAAFCEMVGWTEEELKNVKSPYPFCPGSEVERLREAFARMAENDASQAGFE
                     LCFQHRNGKRFDVMLLIRSLKDAQGKRTGWLASVTDITQRKQAEEALRRAHDELELRV
                     RERTADLEAANVELEKAIQERQRLEHELLDITEKERRRIGLDLHDDLGQKLAGITLMM
                     KGLEVALRRKKSPEADAAKKIEVIIQQTVNHASNLAHNMVLADLPEKDLPTALAGLAS
                     NVKNLFDISCEFKSEGGIPPLEKNIIMQLYKITQEAVTNAVKHGKAREVGINLIGEPD
                     RLILTVRNNGLPFPSMIDQRKGMGLRIMNYRASTIGGSLEVKADRPKGTLVTCSLPTK
                     LKKSAKDKK"
     gene            complement(2118..2576)
                     /locus_tag="VG754_08260"
     CDS             complement(2118..2576)
                     /locus_tag="VG754_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19246.1"
                     /translation="MEPKQFVLLVEDSYNDELLARRAFKKCQMADNLTVVRDGAEALD
                     FLFGAGAHASRDWRQLPAVVLLDLKLPKLSGLEVLRRARADERTRTLPIIVLSSSDEE
                     NDKSESERLGANGYLRKPVEFKEFVETVRQLSLCWLQADGTLKNKQKNPP"
     gene            complement(2587..>3365)
                     /locus_tag="VG754_08265"
     CDS             complement(2587..>3365)
                     /locus_tag="VG754_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19247.1"
                     /translation="LEEQIREREQAEDEVQKLNAELELRVEERTRQLQLANAELEAFA
                     YSVSHDLRGPLRSVAGFTEALKDENEERLDAEGREYLGRVLQSCREMDKLIDDLLHLS
                     RLSRVDMKRQPVNLSLLAEGIVAQLRETEPRRRVKVAVAPSLVAPADARLMRIALENL
                     LNNAWKFTGRKRAAQIEFGMLSGPEGSAYFVRDNGAGFDMAYAGKLFGAFQRLHSTHE
                     FPGHGIGLATVQRIINRHGGRTWATGEVDRGAAFYFTLPS"
BASE COUNT          666 a         1007 c          915 g          777 t
ORIGIN      
        1 gataggagtt tgggggcgca tctcggcttt ttgcgcagcg ggtatttgga tagttccttt
       61 cccctcaccc cggccctctc ccatcgggag agggagcatt atcttccgtc gtggttgcaa
      121 ttgaatgcgc tcgattgcac aagcagcgga caaacggttc gccctctcct tggggagagg
      181 gttggggtga gggggaccac gacgtccgcc agccgcaatt atcaaccgaa aaggaaccgc
      241 agcatccaga aaagcgagat gcgcccggag acgggtgctc agcctttcat acttctcggt
      301 tcatggtttc tacagcaaag tcgcatcaca aacgatttgt atttcccgat gttcgccacg
      361 aaacgagcgc gcgtaaatcg tatatcgtat atcgtaaatc tgctcacttc ttgtccttcg
      421 ccgatttctt caacttggtg ggcaaagagc acgtgaccaa agtgcctttg ggacgatcgg
      481 ctttgacttc gagcgagccg ccgatggtgc ttgcccgata attcatgatg cgcaggccca
      541 tccctttgcg ttggtcaatc atcgagggaa acggcaggcc gttgttgcgg acggtgagaa
      601 tcaatcggtc aggttcgccg atgagattaa ttcccacctc gcgggccttg ccgtgcttga
      661 cggcattcgt gacggcttcc tgcgtgattt tgtaaagctg catgatgata tttttttcca
      721 acggtggaat gccgccttca gacttgaact cgcaggaaat atcgaaaaga tttttcacgt
      781 tcgaagcgag ccctgccaac gccgtgggca ggtccttttc cggcaggtcc gcaagcacca
      841 tgttgtgcgc cagattgctg gcgtggttga cggtctgttg gatgatgacc tcgattttct
      901 tcgccgcatc cgcttcaggc gattttttgc gacgcaaggc aacctcaagc cccttcatca
      961 tgagcgtgat gccggcaagt ttctggccaa ggtcatcgtg caaatccaat ccgattcgcc
     1021 tgcgttcctt ttcggtgatg tcgagcagtt catgttcgag acgctggcgt tcctggatcg
     1081 ctttttccaa ttcgacgttg gccgcctcaa ggtcggctgt gcgttcgcgg acacgcaatt
     1141 ccaattcatc atgcgcgcgc cgcaatgctt cctccgcctg cttgcgctgg gtgatgtccg
     1201 tcactgacgc gagccagccc gttcgtttgc cctgggcgtc cttgagcgaa cgaatgagca
     1261 gcatcacatc aaaacgtttt ccgttgcgat gctgaaaaca taattcaaag ccggcctgtg
     1321 acgcatcgtt ctccgccatc ctggcgaacg cctcgcgcaa tctttccacc tcagacccgg
     1381 gacaaaaagg ataaggcgat ttgacgtttt tcaactcctc ttccgtccag cccaccatct
     1441 cgcagaatgc cgcgttgacg taagtcagcg cgccctggac gtccgtggcg gcgatgcctg
     1501 aagggatcga ggcttcgatg gcgtggcgga aggccaattc ggcggctgct tccttctcga
     1561 tttcgcgccg ctctgcgcga ttggcggctt cctgcatctc acgttcaatg gccgggacga
     1621 gccgcgtcag gcggtccttc atcacgtaat catgagcgcc ggccttcatc gaagcgaccg
     1681 ccgtgtcttc atcaatatgg ccggacacga tgatgaacgg caggtcaagt tcgcgccgtt
     1741 gcatcaactt caacgcggca ggaccgctga acctcggcat cacataatcg gcaatcacaa
     1801 cgtcccacga ttgccggtcc aatgccgcag tcatttccgc ggctgtctcg acccgttggc
     1861 attcgggctg atagccgttt tgccgcagct ccagcagcaa aagcgcggcg tcgagttccg
     1921 agtcttcaac cagcaatacg cgaatggggc ggctcacaag atttggtaag tggagtggtg
     1981 ggttgatgga gtgatggagc aatggaatga aagagttgtg aaagaagttg cgcggacggc
     2041 tcttctttcc catccgcgcc ggcgggcgga aggcaaagct gcatctgtgc ggaattggcg
     2101 gggattattt agacacatca tggcggattt ttttgtttgt tcttcaacgt gccatcagct
     2161 tgaagccagc acaaactcaa ctggcgcact gtttcgacga attctttaaa ttccactggt
     2221 tttcgcaaat aaccattggc cccgaggcgc tcgctctccg atttgtcgtt ttcctcgtcg
     2281 gaggaactca acacgatgat tggcaacgtc cgggtccgct catccgcgcg cgcccggcgc
     2341 agcacttcaa ggccgctcaa tttgggcagc ttcaaatcca gcaggaccac ggcgggcaat
     2401 tgccgccaat cccggcttgc gtgcgccccg gcgccaaaca ggaaatccag cgcttccgcg
     2461 ccgtcccgca ccacggtcag attgtcagcc atctggcatt ttttgaaggc ccgccgcgcg
     2521 agcagttcgt cgttgtagct gtcttccacc agcaggacga attgttttgg ttccatgtgt
     2581 gtttggtcac gacggtaaag taaaataaaa agcggccccg cgatcaacct cgcccgttgc
     2641 ccaagtcctg ccgccatggc gattgatgat gcgctggaca gtggccaggc cgatgccatg
     2701 gccgggaaat tcatgcgtgc tgtgcaggcg ttggaaagcc ccgaacaatt ttccggcgta
     2761 cgccatgtcg aagcccgcgc cgttgtcccg cacaaaataa gccgatcctt ccggcccgga
     2821 caacattcca aactcgatct gcgccgcccg tttccgtccg gtgaatttcc atgcgttatt
     2881 caaaagattt tccagcgcga tgcgcatcag gcgcgcgtcc gcaggcgcga ccagacttgg
     2941 cgccactgca accttcacgc gccggcgcgg ctccgtttca cgcaactgcg cgacgatgcc
     3001 ttccgccagc aagctcaagt tcaccggctg ccgtttcatg tccacgcgcg agaggcgcga
     3061 aagatgcaaa aggtcgtcta tgagcttgtc catttcgcgg caagactgga gcacgcggcc
     3121 cagatattcc cggccttccg catcaagccg ttcctcgttc tcatccttca acgcctcggt
     3181 gaaacccgcg acgctccgca acggcccgcg caaatcgtgc gacacagaat aagcgaacgc
     3241 ctccagttcc gcattggcaa gctgcaattg ccgcgtccgt tcctccacgc gcaattccag
     3301 ctccgcgttg agtttttgga cctcgtcctc cgcctgctcc cgctcgcgga tttgttcctc
     3361 caaaa
//
LOCUS       DASZMM010000341         8348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2613336, whole genome shotgun sequence.
ACCESSION   DASZMM010000341 DASZMM010000000
VERSION     DASZMM010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613336"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VG754_08270"
     CDS             <1..418
                     /locus_tag="VG754_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWF19248.1"
                     /translation="FKGNEQNYYDPENSYLNRVVDRRLGNPINLSLVYLLLARRLKLP
                     VTGIGLPTHFICRYQSTSEEYYIDVFNRGKLWTKADCVQFLLSRNYPLRDEYFAPVNP
                     RQILMRVCGNLHQIYQHLEMPEESTRLQRYLIALAK"
     gene            complement(438..1586)
                     /locus_tag="VG754_08275"
     CDS             complement(438..1586)
                     /locus_tag="VG754_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase B"
                     /protein_id="HWF19249.1"
                     /translation="MMTLAFKAGSYKWLQGGDEIFPALAASLNGARKTIRFEIYIYAN
                     DELGQHFRDLLVQSSARGVRVQVLIDSLGSLGLSTAFWKPLTETGGEVRWFNPDIPKR
                     FSARDHRKILVCDEEVAFVGGFNMAVPYQGDGVKSGWLDLGLRIVSPLAVQLAAAFDE
                     MFAGASCQPQEYSHPHSKPMAAKRKVAVDEGELLLSGPGRGRNPFNRSLRRDLDGARN
                     VQIIAAYFLPTWRIRRSLIFAARAGGKVQLILPAKSDVLLSQLACRSLYRRLLRAGVE
                     IYEYQPQILHAKLIIIDGVVYVGSSNLDPRSLYINYELMLRFQKEKLVVEARALFAEN
                     LKHCQRIELESWRKSGTWWSKLKQRWAYFLLVRVDPVIARWQTSRVRH"
     gene            1640..3778
                     /locus_tag="VG754_08280"
     CDS             1640..3778
                     /locus_tag="VG754_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HWF19250.1"
                     /translation="MKLHRLLWTTLLVVLFAGTAHGAATDTSLILGSQAARPGDTVMA
                     GIRLRMKPGWHVYWRNPGGSGLPVSITWHLPNGFTAGEIQWPVPAKLPAEELTTYIYE
                     DETVLLVPLKIALDAPFGQVELKANVSWLECKTQCVPGSGDVQAFLTVGDAAKPSPDA
                     TLIAEWQKKLPLKKPDLGARAFWEGPPNGDTRGIIFEWPAKGTVKDADFYPYSSDKSD
                     VQWKNTFVPAEGGMLRLKKIVKKLEGNWPDNISGLLVQKLEDRTLAYEVNLPISETGI
                     PPATSKSAPLSGAAARPPQRFSLVLLYAFLGGIILNVMPCVLPVIALKILGFVNQSKE
                     HPRHVRKLGLLYAAGVLVSFLALAVLVIFVKQLGHAASWGMQFQNPQFLVLMTALVSL
                     IALNLFGVFEINLGGSVMGTAGKLASKEGSAGAFLNGLLATILATPCTAPFLGPALGF
                     AFTQPAGIIILVFLTVGLGLAAPYVILSWEPAWLKFLPKPGAWMEKFKVAMGFPMLAT
                     AIWLFTLTTPFFGEDGDLWLGMFLVLIGLGAWVWGQFVQRGRARRALAVGVSLGIFLI
                     AYGYLLERQLDWRHPRMVAKGSLQNAPDGIDWQPWSPDALKEARAAGRPVLVDFTAKW
                     CLICQLNKREALEIPKVRAKLKQIGAVALEENSYTKDPVVIAELNRYQRAGVPLVLIY
                     PKDPNAAPEVLPELLTPNIVLSALDKAVAN"
     gene            complement(4032..5876)
                     /locus_tag="VG754_08285"
     CDS             complement(4032..5876)
                     /locus_tag="VG754_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWF19251.1"
                     /translation="MKFCNQAGKLGRVIAISICWFSALCVAHTQTSGKFEERYSRLIQ
                     NPSHETDAARLKELFDINWEYLMTEYPEWATDVGYPGQNRRWTDNSLEAIERRKRETE
                     NPLQVIKTIDRSKLDAADQLNYDLFKRSLEHSIEGDRFKGEYLAINQLGGVQQDVPQM
                     LEVIMPHRSVQDYEDILARLKTVPTVISQTLVLLKRGLETGITPPRVTLRDVPQQVES
                     QLVEDPQENPMLQPFNEFPAAILPNEQKRLRSEAASILTNGVVTAFRELHEFLVKTYL
                     PGARESIGMSDLPDGQAWYAFNVRERTTTELTPKQIHELGLSEVKRIRKEMDDVIKES
                     GFKGSFEEFNQFLRTDPQFYFTNANDLLTGYRDICKRADPELIKLFGKLPRLPYGVLP
                     VPKYSEKSQTTAYYQGGSLVAGRPGYFYANTYALNTRPKWEMQPLALHESVPGHHLQL
                     SLALELPDVPEFRKYEGYTAFVEGWGLYSEGLGSEMGFYKDPYSKYGRLTYEMWRAIR
                     LVVDTGIHSMGWSRQQAIDYFKANAGKNEHDIIVEVDRYIVWPGQALAYKIGELRFKE
                     LRAYATKELGPKFNIRAFHDELLSNGALPMDILEKHMREWVGKQKKPI"
     gene            6027..6485
                     /locus_tag="VG754_08290"
     CDS             6027..6485
                     /locus_tag="VG754_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19252.1"
                     /translation="MKPEKNKAVFLLVEDDPNDAFLVEREFAKANQTVRLEVVNDGQE
                     AVHYLKGEGQYGDRDAHPMPNLLLLDLKMPRLGGFEFLKWLRSKSAENIRLIPVVVLS
                     SSHLEEDVERAYNLGANSYMVKPLNWDEFRKLLVDLGIYWTKHALTPQFR"
     gene            complement(6478..6957)
                     /locus_tag="VG754_08295"
     CDS             complement(6478..6957)
                     /locus_tag="VG754_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19253.1"
                     /translation="MKKMILLADDSIDDELFFKHVLKAAGVENPVHVVRDGKEVIAYL
                     KGEGPYADRERFPRPVVLFLDLSMPGMDGWEVLKWLQSQPNRKDLLVIVLAGSSQRRR
                     LREVYEMGANSFLFKPFSKAELEGLIFHWPDAWTISKPEPPMPPPPPSQQPGNRPAI"
     gene            complement(7269..>8348)
                     /gene="icd"
                     /locus_tag="VG754_08300"
     CDS             complement(7269..>8348)
                     /gene="icd"
                     /locus_tag="VG754_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWF19254.1"
                     /translation="VGIKGPLTTPVGGGIRSLNVALRQMLDLYVCLRPVQYFPGVPSP
                     VKHPEKVEMVIFRENTEDIYAGIEYAGGTPEAQKVLDFLAQTFPKDFAKIRFGTKDAI
                     AGYMKMASPDHDASHIPVQVGLGIKPISNVGTIRLIKSAIEYAVREKRKSVTIVHKGN
                     IMKFTEGAFRDWGYSAAERIFGDKVYTWAKWERTKKEKGEMAANEEQKAALKAGAVLV
                     KDSIADITLQQVLTRPEDFDVIATPNLNGDYLSDALAAQVGGIGIAPGGNINYVTGHA
                     IFEATHGTAPKYANLDKVNPGSVVLSGEMMFRYLGWTEVADMIIKGLNGAIGSKRVTY
                     DFARMMEGAKEIKCSEFGDNIITHM"
BASE COUNT         1851 a         2301 c         2211 g         1985 t
ORIGIN      
        1 gttcaaaggc aatgaacaaa attattacga cccggagaac agttatctga accgcgttgt
       61 ggaccgccgc ctgggcaacc ccatcaatct cagccttgtt tatcttttgc tggcgcgccg
      121 gttgaaactg ccggtgactg ggattggatt gcccactcat ttcatctgcc gttatcaatc
      181 cacgtccgag gaatattaca tagacgtgtt caaccgcggc aaactctgga ccaaggccga
      241 ttgcgtccaa tttcttcttt cacgcaacta tccgctgcgg gatgaatact tcgctcctgt
      301 gaatccgcgt cagattttga tgcgggtttg cggcaacctg caccagattt atcagcacct
      361 cgaaatgcct gaggaaagca cccgtctcca acgttatctg atcgcattgg caaaataatt
      421 caattctttg cgtcgcttca atgtctcacg cggcttgttt gccatcgggc aatcaccggg
      481 tcaacgcgca ccaacaaaaa atatgcccaa cgttgcttga gcttgctcca ccaagtgccc
      541 gacttgcgcc agctttccaa ttcaatccgc tggcaatgtt tcaagttctc cgcgaacagc
      601 gcccgcgcct cgacaaccag cttctccttc tgaaaacgca gcatcagttc gtagttgatg
      661 taaaggctgc gcggatcaag gttcgacgag ccgacataaa caacgccgtc aatgatgata
      721 agcttggcgt gaaggatttg cggctggtat tcgtagattt ccacgccggc cctgagcaac
      781 cggcgataaa gactgcggca ggcaagttga gaaaggagca cgtccgattt tgcaggcaga
      841 atcaactgca cctttccgcc cgccctcgca gcgaaaataa gtgaacgacg aatccgccac
      901 gtcggcaaaa aataagccgc gatgatctgc acgttgcgcg cgccgtcaag atcgcgccga
      961 agagagcggt tgaaaggatt gcgaccgcgt cccgggccgc tcagcaacag ttcgccttcg
     1021 tcaacggcca ctttgcgttt tgctgccatc ggtttggaat gcggatggga atactcctgc
     1081 ggttgacaac tggcgcccgc gaacatttcg tcgaaggccg cggcgagttg aactgccagc
     1141 ggactgacaa tccggaggcc gagatcaagc cagccggatt tgacgccatc accctggtat
     1201 ggcacggcca tgttgaatcc accgacaaac gcgacctcct cgtcacaaac cagaatcttg
     1261 cggtggtcgc gcgcgctgaa gcgcttggga atatcaggat tgaaccagcg gacttcacct
     1321 ccggtctcgg tgagcggttt ccaaaaagcc gtggaaaggc ccaaggaacc gagcgagtca
     1381 atcaacacct gcacgcgaac gcctctcgca gaggactgga caagcaggtc gcggaaatgc
     1441 tggcccaatt catcgtttgc gtagatgtaa atctcgaaac ggatggtttt gcgcgcgccg
     1501 ttcaggctgg cggcgagcgc gggaaagatt tcatctccgc cctggagcca cttgtatgag
     1561 cccgccttga acgccaacgt catcatgatg aatgtataat aaagtggagc gtttcgcaat
     1621 tttagtgcag gatatgaccg tgaaacttca caggctcttg tggacgacat tgctcgtggt
     1681 tctcttcgcg ggaacggcgc acggcgcggc cacagacacc agtcttattc tcggctcgca
     1741 agcggcgcgt ccgggcgaca ccgtgatggc gggcatccgc ctgcgcatga aaccgggctg
     1801 gcatgtttac tggcgcaatc cgggcggttc gggattgccg gtgagcatca cctggcatct
     1861 gccgaatgga ttcaccgcgg gagaaatcca atggccggtc ccagccaaac ttccggcgga
     1921 agaattgacg acctacatct acgaagatga aaccgtcttg ctcgtgccgc tgaaaatcgc
     1981 cctcgacgcc ccattcggcc aggttgaatt gaaggcaaac gtaagctggc tcgaatgcaa
     2041 aacccaatgc gttcccggct ccggagatgt ccaggcattt ttgaccgtgg gtgatgctgc
     2101 gaagccttcg ccggatgcca cgttgattgc cgaatggcaa aaaaaacttc cgctgaaaaa
     2161 acctgatttg ggcgcgcgcg cattttggga gggacctccg aacggcgaca cacggggaat
     2221 catctttgaa tggccggcaa aaggcacagt caaggacgcc gatttttatc cttactccag
     2281 cgacaaatcc gacgtgcaat ggaaaaatac ctttgtgccc gcggaaggcg gaatgctgcg
     2341 gcttaaaaaa atcgtcaaga aacttgaagg caattggccc gataatattt ccggattgct
     2401 tgttcaaaaa ttggaagacc gaacattggc gtatgaagtg aatctgccga taagcgaaac
     2461 cggaatcccg ccagcgacat ccaaatctgc gccgctgtcc ggcgcagccg cgcgcccgcc
     2521 gcagcgattc agtcttgtgc tgctttacgc gttcttgggc ggaatcattt tgaacgtcat
     2581 gccatgcgtc ttgccggtga ttgcgttgaa aatcttgggg ttcgtgaatc aatcgaagga
     2641 acatccccgc catgtgcgaa aactcggctt gctctacgcg gccggcgtgc tggtgtcttt
     2701 tttggcactg gccgtattgg tgattttcgt gaaacaactc ggacacgcgg cgagttgggg
     2761 aatgcaattt cagaacccgc aatttctggt gttgatgacc gcgctggttt ctttgattgc
     2821 cttgaatctg ttcggcgtgt ttgaaatcaa tctcggcggt agcgtgatgg gaaccgcagg
     2881 caagctggct tcgaaggaag gttccgcagg cgctttcctg aacggtctgc tcgccaccat
     2941 tttggcgacg ccctgcactg ccccgtttct cggccccgct cttggattcg ctttcacgca
     3001 accagcaggc atcattattt tggttttcct gaccgtcggc ctcggcttgg cggcgccgta
     3061 tgtcatcctt tcctgggaac cggcatggct taaatttctt cctaaacccg gcgcgtggat
     3121 ggagaaattc aaagtggcaa tgggttttcc gatgctcgca acggccatct ggttgttcac
     3181 gctcaccact ccgttcttcg gcgaagatgg ggatttgtgg ctcggaatgt ttttggtgct
     3241 gatcggtctt ggcgcatggg tttggggaca atttgtgcaa cgcggccgcg cacgacgcgc
     3301 tttggctgtg ggggtgagcc tgggaatatt tttgattgcg tatggttatt tgctggagcg
     3361 gcagcttgac tggcgtcatc cgagaatggt cgcaaagggt tcgttgcaaa atgcgccgga
     3421 cggaattgac tggcagccat ggagcccgga cgcgctcaag gaagcgcgcg ccgcaggccg
     3481 gccggtgctg gtggatttca cagcgaagtg gtgtttgatt tgccagttga acaagcgtga
     3541 agctctggaa atcccgaaag tgcgcgccaa gctcaaacaa atcggcgcgg tcgccctgga
     3601 agaaaacagt tacacgaaag acccggtggt catcgcggaa ttgaaccgtt atcaacgcgc
     3661 cggcgtgccg ttggttttga tttatccgaa agatcccaat gcggcccccg aagtccttcc
     3721 cgaattgctc acaccgaaca ttgttctgag cgcgctcgac aaggccgttg caaattaact
     3781 cggagaattc caattgttcc gtgccttgct ctgtagaaac catcatgtga catgcataaa
     3841 aaggcgaagc accatttgtc cgctgcttgt gcagtcggac acattgaatt gcaaccttct
     3901 cgtgtctctc ggctgacatt gactcagcca agggaattga acgggttatt aaaaaatgac
     3961 cttcaattgc cctggtctga ccgctggaaa tcgcacgttg tgttctgttg cggctcgcca
     4021 agagccttga attagatcgg ctttttctgt tttcccaccc attcgcgcat gtgcttttcc
     4081 aggatgtcca tcggcaatgc gccgttgctg agcaactcat cgtgaaacgc gcgaatgttg
     4141 aatttcgggc ccaattcttt tgtcgcgtag gcgcggagtt ccttgaatct cagttcgccg
     4201 attttatacg ccagcgcctg gccgggccag acaatgtaac ggtccacttc gacgatgatg
     4261 tcgtgttcgt tcttgcctgc gttggccttg aaataatcga ttgcctgctg gcggctccag
     4321 cccatcgaat ggattccggt gtccaccacc agccggatgg cgcgccacat ttcgtaagtc
     4381 aaccggccat attttgaata ggggtccttg taaaagccca tttcgctgcc gaggccctcc
     4441 gagtaaagcc cccacccttc cacgaacgcg gtgtaacctt catacttccg aaattccggc
     4501 acgtccggaa gttcaagcgc gagcgacaac tgcagatgat gccccggcac ggattcgtgc
     4561 agcgccaacg gttgcatttc ccatttcgga cgcgtgttca aggcatacgt gttcgcgtaa
     4621 aaatatcctg gtcgtcccgc gaccagcgaa ccgccctgat aataggccgt cgtttgcgat
     4681 ttctccgaat acttggggac gggcaaaacg ccgtagggaa gccgcggcaa tttgccgaac
     4741 aatttgatca actccggatc ggcgcgtttg cagatgtccc ggtaccccgt caacaaatcg
     4801 ttcgcgttgg tgaaatagaa ttgcggatcg gtccgcaaaa attgattgaa ctcctcgaag
     4861 ctgcctttga atccgctttc cttgatgacg tcgtccatct ccttgcggat gcgcttcact
     4921 tcgctcaagc ccaattcgtg gatctgcttt ggcgtcagtt cagtggtcgt ccgctcgcgc
     4981 acgttgaacg cataccacgc ctggccgtcc ggcaaatcgc tcatgccgat gctttcacgc
     5041 gcgcccggca aataagtttt gaccaggaat tcatgcagct cgcgaaacgc cgtgacgacg
     5101 ccattggtca aaatggacgc cgcttcgctc cgcaaccgtt tctgctcgtt tggcagaatc
     5161 gcggccggaa attcgttgaa cggctgcagc atcggatttt cttgcgggtc ttcgaccaac
     5221 tggctttcca cctgttgggg aacgtcgcgc aacgtgacgc gcggcggcgt gatgccggtt
     5281 tccaatccac gtttcaagag cacaagggtc tggctgatga ccgtgggcac tgtcttcaac
     5341 cgggcaagga tgtcctcata atcctgcaca ctccggtgcg gcattatgac ttccaacatc
     5401 tgcggcacat cctgttgcac gccgccaagc tggttgatgg cgagatactc gcccttgaac
     5461 cggtcgccct cgatggagtg ttcaaggctc cgtttgaaaa ggtcgtaatt gagctggtcg
     5521 gccgcgtcca atttcgagcg atcaatcgtc ttgataactt gcaggggatt ttctgtttca
     5581 cgtttgcgcc gctcgatggc ttccagcgaa ttgtccgtcc aacgccggtt ttggcccgga
     5641 tagccgacgt ccgtcgccca ctccggatat tcggtcatca gatactccca attgatgtcg
     5701 aacaattcct tcaaccgcgc ggcatcagtt tcgtgggatg gattttgaat caaccgcgag
     5761 taacgttcct cgaacttgcc gctggtctgt gtgtgagcga cacaaagcgc tgagaaccaa
     5821 caaatactaa tcgctatgac ccggcccaac tttccggctt gattgcaaaa tttcattgtt
     5881 gccaaacatg ttgcgccatt ttcaggagga ttcaaccatt tcctgataaa tcgaaagagt
     5941 gcttgggtag tttaccccgt ttgcggcttc accgttcagt gggtacattt ttacgttgag
     6001 caatcaaatg acggcttgga caggacatga agcccgaaaa aaacaaggca gtgttcttgc
     6061 tggtcgaaga cgatccaaac gacgccttcc tcgtcgaacg tgaatttgca aaggccaatc
     6121 aaaccgttcg gctggaagtc gtcaacgatg gacaggaggc ggtccattat ttgaagggtg
     6181 agggccaata cggtgatcgc gatgcccatc caatgcccaa tcttttgttg ctcgacttga
     6241 aaatgccgag gctgggcgga tttgaattcc taaaatggct tcgctcaaaa tcggcggaga
     6301 acattcggtt gattccagtg gtcgtgcttt cttcctcgca tctggaagag gacgtggagc
     6361 gcgcctacaa cctgggagcc aattcttaca tggtcaaacc gctcaattgg gacgagtttc
     6421 gcaagttgct ggttgacctc ggcatctact ggacaaaaca cgcgctcacg ccgcagttca
     6481 gatagcaggc ctgttgccgg gttgctggga gggaggtggc ggcggcattg gcggctcggg
     6541 tttggaaatc gtccacgcat cgggccaatg gaaaatcaat ccttcgagtt cagccttgct
     6601 gaaaggtttg aacaaaaaag aatttgcgcc catttcgtaa acttcgcgca atcttctgcg
     6661 ctggctggaa cccgccagca cgatgacgag caaatctttc cggttcggtt gcgattgaag
     6721 ccatttcaaa acttcccatc catccatgcc cggcatcgac agatccaaaa aaagcacgac
     6781 gggacggggg aatcgttcgc gatcggcata aggtccttcg cccttgaggt acgcaatgac
     6841 ttccttgcca tcacgcacta catgaacggg attctcgacc ccggcggctt tcaagacatg
     6901 cttaaaaaat aactcgtcgt cgattgaatc atcggccagc aggatcattt tcttcattaa
     6961 tttagcactt catcttttta accaaatgac actcatccat ggagtgctcc aatggggatt
     7021 tcacctttat catcatggcc ccaagaccca ttacttccac ttacttacaa atcggccatg
     7081 aggatgcatg ttaatgcagc tattgcagtg acgcaaatta aaaacgtgta ctattcacaa
     7141 attttcagac cacacgcgcc acttgcaaaa agagaattta cgtgatgcaa attgcattat
     7201 taagaattgc taaaaacaaa aaacccgggc ttttgcggct ccgggtttgc attttgcaaa
     7261 aaacgaattt acatgtgagt aatgatgttg tcgccgaatt cggaacattt gatttccttc
     7321 gcgccctcca tcatgcgcgc aaaatcataa gtgacgcgct tggaaccgat tgccccattc
     7381 aagcctttga tgatcatgtc cgcgacttca gtccagccga gatagcggaa catcatctcg
     7441 ccgctcaaca ccacgctgcc cggattaacc ttgtcgaggt ttgcgtattt tggcgcggtg
     7501 ccgtgcgtcg cttcaaagat ggcatggccg gtcacgtagt tgatatttcc tccgggcgcg
     7561 atgccgatgc cgccgacctg cgccgcgagc gcgtcagaaa gataatcgcc gttcaaattc
     7621 ggcgtggcga tgacatcgaa atcttccggc cgcgtcagca cttgttgcaa cgtgatgtcc
     7681 gcgatggagt ccttgaccag gacggcgccg gctttcaacg ccgctttctg ctcctcgttc
     7741 gcggccatct cgcccttttc ctttttggtg cgttcccatt ttgcccaggt gtaaaccttg
     7801 tcgccgaaaa tccgttccgc agccgagtaa ccccaatcac gaaacgcgcc ttcggtaaat
     7861 ttcatgatgt tccccttgtg aacgatcgtg acgctcttcc gtttttcacg taccgcatat
     7921 tcgatggccg acttgatgag ccgaatcgtc cccacgttcg agatcggctt gatgcccagg
     7981 ccaacctgca cggggatatg cgaagcgtcg tggtcgggcg aagccatctt catgtaaccc
     8041 gcgatggcgt ctttcgtgcc gaaacggatt tttgcgaaat ctttcggaaa agtttgcgcg
     8101 agaaaatcga gcaccttctg cgcttcgggt gttcccccgg cgtattcgat gcccgcgtag
     8161 atgtcctccg tgttctcgcg gaagatgacc atttccactt tttccggatg cttcaccggc
     8221 gacggcacgc ccgggaaata ttgcaccggc cgcaggcaaa cgtagaggtc cagcatctgg
     8281 cgcaacgcca cgttcagcga acggatgccg ccgcccacgg gagtggtcaa cggccccttg
     8341 atgccgac
//
LOCUS       DASZMM010000342         5706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_428407, whole genome shotgun sequence.
ACCESSION   DASZMM010000342 DASZMM010000000
VERSION     DASZMM010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428407"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VG754_08305"
     CDS             <1..1470
                     /locus_tag="VG754_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HWF19255.1"
                     /translation="HPDPSIIRVGKDYWATSTSSEWGPQFPLLHSRDLVNWEQTGEVF
                     EHRPTWAVGNFWAPEISHFNGRYFVYYVGREKDGPLAVAVATADKPSGPYKDHGPIIA
                     QEQGSIDPAPCLDENGHPYLIWKDDNNSRRKQTTIWAAPLDADGLKLTGEPTALIHND
                     EDWEGALVEGPYILRRGNWFYLFYSGSGCCGSGCNYALGVARSHSLLGPWEKNPHNPI
                     LTGNDVWKCPGHGSIVSDEKGRYWLLYHSYSTKSSVYTGREAMLDEVKFGPDDWATIN
                     DGKGPSVQAVSPFGAKQTRRNLSFTDDFKGSHLRHGWQWPQGLEPTYQLRGDHLVLLA
                     SPGHETNALAAILARSTMTGNYIAETEIEAADLQPGSAAGLSAFGDPNNAVGLAEGDG
                     RLILWRREHGNYSRLAEMDAPKAKKLYLRLTASHGYQFHFAASVDDKNWIPVGENLTG
                     KNLPPWDRSLRVALTVEGGEGAEARFDSFRLTPEVASHN"
     gene            1556..1819
                     /locus_tag="VG754_08310"
     CDS             1556..1819
                     /locus_tag="VG754_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19256.1"
                     /translation="MPSEPVNLTVDEIRELNQRLSTMRHDINNNLSLLIAATELMRAK
                     PQMVEKMLDTLCAQPPKISAAMTKFSAEFEKAFHIEREAKKPQ"
     gene            1902..2477
                     /locus_tag="VG754_08315"
     CDS             1902..2477
                     /locus_tag="VG754_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWF19257.1"
                     /translation="MELSDAELIAAVLNGDTASFEPLVQKYSPRVFATARRYARRESE
                     IEDIVQEVWLKSFQKLASFRGEAPFEHWLMRLAVRTCYDFLRGHQRNRETAFSEMTDT
                     EEDWLERFVTHPEEAAESAAAARQLVERVLEQLSPPARLIITLLEIEDRSIKEIAQLT
                     GWSVPLVKVRAFRARAEMKKCLARLAKDKYL"
     gene            2517..2840
                     /locus_tag="VG754_08320"
     CDS             2517..2840
                     /locus_tag="VG754_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19258.1"
                     /translation="MNLAELQRKLIAAARANAPEDRVPYAFEKRIMARLAGQKAPDVW
                     ALWGRAFSRAAIYCVAFMLMLAAGSYFAPPPPASGSGNSVSLSQDVDQTLFAAVDNNS
                     DQETW"
     gene            2844..3332
                     /locus_tag="VG754_08325"
     CDS             2844..3332
                     /locus_tag="VG754_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19259.1"
                     /translation="MNAWKVILSTLVIFIAGIVTGIVLVTFEERLHPKAQPRPVVVKT
                     NAYNNAGGNNPWMMRNVQLLRRMDRELDLTPEQHTHIEAIIKESQERIKGLMRSVQPL
                     MNKEMQETRSEIIKELTPEQQKKFEDFPHHPNAERRHGETNTAPINQSATGTNSVSTN
                     AP"
     gene            complement(3365..5638)
                     /locus_tag="VG754_08330"
     CDS             complement(3365..5638)
                     /locus_tag="VG754_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWF19260.1"
                     /translation="MNHPETTSPHATDLLVTGMTCNNCARHVAEAIQSVSGVASASVQ
                     LNAGRATVRWKDGSNLPEVLRAVSEAGYEAKPLDEQTVAAEAKTKWSPLAGWRFNVVV
                     GLICTLPLIIGEWAFGLGMERWFRWLSFALALPVQIFCGAKFYVGAWRQLKVGASNMD
                     TLVALGSTTAFVYSVWALFTGTPGHLFFMESAAIITIVSLGHWFEARTSTRAESALQA
                     LLHLAPEMARRCNPDGSEAEVPVRELQLTDTVVLKPGDHVPVDGEVTEGAGAVDESML
                     TGESLPVEKTPGEKLYAGTVNLNGRILMRVTGTGEETALAHIIAAVARAQNSRAEIQR
                     LGDRVSSVFVPVVVLIALGTGLWWGLADEEARQTSEWLSKFLWPAVLPASALAAAFIQ
                     SAAVLIIACPCAMGLATPVAIMAGANAAARRGILVRDGVALERAGRITAIVFDKTGTL
                     TQGKPDVAAQEVYASAPDAMKLASALARRSNHPLSQAVAKLHGEEFPLEDFEEVRGAG
                     VQGGMSAERGVRSAEYGILRLGSLQWLQEMGVDLKPAKAFVEKWSGQGATLLGFAADK
                     TLLGMLAVKDTLKPGADEVMRQLQQDGLKTFLVTGDNALTAQAIAKQAGIPGQNVFAE
                     VRPEQKAQFIQDLRNRGERVAFVGDGINDAPALKAADLGIAVSRASDVARAAADMILL
                     KSEIQAVPEALGLARASLRTIKQNLFWAFFYNAAGIPLAALGFLSPLVCALAMGMSDL
                     VVIGNALRLLRWKMRGK"
BASE COUNT         1242 a         1762 c         1546 g         1156 t
ORIGIN      
        1 catcccgatc catccatcat tcgcgtgggc aaagattatt gggcgacctc gacttcgtcc
       61 gaatgggggc cgcaatttcc gctgttgcat tcgcgagact tggtgaattg ggagcagacc
      121 ggcgaggtct ttgagcatcg cccgacatgg gctgtgggca atttttgggc gccggaaatc
      181 tcccatttca atggacgtta tttcgtctat tacgtaggcc gcgaaaagga cgggccgctc
      241 gctgttgctg ttgccactgc ggacaaacct tccggccctt acaaggatca cggccccatc
      301 atcgcgcagg aacaaggctc gattgatccc gcgccgtgcc tggacgaaaa cggtcatcct
      361 tatctcattt ggaaggatga caacaacagc cgccgcaagc agacgaccat ctgggccgcc
      421 cccttggatg cggacgggct caagctcacc ggcgaaccga cggcattgat ccataacgat
      481 gaggattggg aaggcgcgct ggttgaaggc ccatacattt tgcggcgtgg caactggttt
      541 tatttgtttt attccggcag cggttgttgc ggttcgggtt gcaattacgc tttgggtgtc
      601 gcccgttcgc attcgttgtt ggggccgtgg gaaaagaatc cgcacaatcc aattctcacc
      661 ggcaatgatg tttggaaatg tcccgggcac ggaagcatcg tgagcgatga aaagggccgt
      721 tactggttgc tgtatcactc gtactcgaca aagagttcgg tctataccgg acgcgaagcg
      781 atgctggatg aagtgaaatt tggcccggac gattgggcga caatcaatga cgggaaaggc
      841 ccgagtgtgc aggccgtttc tccgtttggc gccaaacaga cgcgcagaaa cttgagcttc
      901 acggatgatt ttaaaggcag ccatttgcgc cacggctggc aatggccgca aggactcgag
      961 cccacatatc aattgcgcgg cgaccatctt gtgctcctgg caagccccgg tcatgagacc
     1021 aacgcgctcg ccgccatcct cgcccgctcg acgatgactg gtaattatat cgcagagacg
     1081 gagattgaag ccgcggattt gcagccggga agcgcagccg gtttgtcggc gtttggcgac
     1141 cccaacaacg ctgtcggcct ggccgagggc gacggccggc tcattttgtg gcggcgcgaa
     1201 catggaaatt attcgcgtct tgcggagatg gacgcgccca aggcgaagaa gctttatctt
     1261 cggctcacgg ccagtcacgg gtatcagttt catttcgcgg cgagcgtgga tgacaagaat
     1321 tggattccgg tcggcgaaaa tttgaccggt aaaaatttgc cgccatggga ccgaagcctg
     1381 cgcgtggcgt tgaccgtcga gggcggcgaa ggcgctgagg ctcgttttga ttcatttcga
     1441 ttgacgccgg aggttgcatc gcacaattga tatgccgggg agataccgac aacattctcc
     1501 gtactataac gacttgattt tgccatattt cgcaacgatg attgagccaa tgcaaatgcc
     1561 gagcgaacca gtcaatctta ccgtggacga aattcgggaa ctgaaccaaa ggctgtccac
     1621 catgcgccat gacatcaaca acaatctttc ccttctcatc gcggcgacgg agttgatgcg
     1681 ggccaaaccg cagatggtcg agaagatgct ggacacgctt tgcgcgcaac cgcccaagat
     1741 ctccgccgcc atgacaaaat tttcggccga gttcgagaag gccttccata tcgaacgcga
     1801 ggcgaaaaaa ccccagtaac gagttcgggt tttttcgcgc tgggttttaa acgccattgg
     1861 gcattggact ttgtcgcttc agttggcata ctttcgggct gatggaactg agcgatgcgg
     1921 aactgattgc cgccgtgttg aacggtgaca ccgccagttt cgagccgctc gtccaaaaat
     1981 attcgccccg cgttttcgcc accgcccgcc gttacgcgcg gcgcgaaagt gaaatcgagg
     2041 acatcgtgca ggaagtctgg ctcaagtcgt tccaaaaact cgccagcttc cgcggtgaag
     2101 cgccgttcga gcattggttg atgcgtctgg cggtgcgcac ctgctacgat tttctgcgag
     2161 gtcaccagcg taatcgcgag acggctttct cggagatgac ggacacggaa gaggattggc
     2221 tggagcggtt tgtcacgcat ccggaagagg ccgccgaaag cgcggcggcg gcgcggcaat
     2281 tggtcgaacg cgttttggaa cagctctcgc ccccggcgcg tctgatcatc acgctcttgg
     2341 aaatcgaaga ccggtccatc aaggaaatcg cccagttgac cggatggtcg gtgccgttgg
     2401 tgaaagtccg cgcgtttcgc gcccgcgcgg agatgaaaaa gtgtctggcg cgactggcca
     2461 aagacaagta tttgtaacca caggtctgtg aaaaacgtct ggttggatga aggcttatga
     2521 accttgcaga attgcaacgt aaattgatcg cggccgcccg ggcaaacgct ccggaagacc
     2581 gtgtccccta cgcctttgaa aagcgcatca tggcacgttt ggcgggccaa aaagcgccgg
     2641 atgtctgggc gctttggggc cgcgcctttt cgcgcgcggc gatctattgc gtcgcattta
     2701 tgctgatgct ggcggcaggt tcgtatttcg cccctcctcc accggcttcc ggttcgggaa
     2761 attccgtaag cctttctcag gacgtggatc agaccctgtt tgccgccgtt gacaacaaca
     2821 gcgatcagga aacctggtga acggtgaatg cctggaaagt catcctctcc acacttgtca
     2881 ttttcatcgc cggtattgtg accggcatcg tcctggtgac ttttgaagag cgccttcatc
     2941 caaaggcgca gccccggccg gtggtcgtca aaaccaacgc ctacaacaac gctggtggca
     3001 acaacccttg gatgatgaga aatgtgcaac tgctccggcg catggaccgc gagttggatt
     3061 tgacgcccga acaacacacg cacatcgaag ccatcatcaa ggaaagccag gagcgcatca
     3121 aaggtttgat gcgttcggtg caaccgctca tgaacaagga gatgcaggaa acgcgctctg
     3181 aaatcattaa ggagctgact ccggaacagc aaaagaaatt cgaagatttc ccccaccatc
     3241 caaatgccga aaggcggcac ggggaaacca acacagcgcc catcaatcag tccgccaccg
     3301 ggaccaattc ggtttccacc aatgctcctt agcggctgtc gattggcaac cattttcgcg
     3361 gcgttcattt tcccctcatt ttccaacgca gcaatcgcag cgcattgccg atcaccacga
     3421 gatccgacat ccccatcgcc aaagcgcaga ccaacgggct caaaaatccc aatgccgcca
     3481 agggaatgcc cgccgcgttg taaaaaaatg cccaaaataa attctgtttg atggtgcgca
     3541 acgacgcgcg cgccaatcca agtgcttccg gcaccgcctg gatttccgac ttcaacaaaa
     3601 tcatgtccgc agcggcgcgt gccacgtcgc tcgcgcggct cacggcgatg cccaggtccg
     3661 ccgctttcaa cgccggcgca tcgttgatgc cgtcgcccac aaacgccacg cgctcgccgc
     3721 gattgcgcaa atcctggatg aactgcgcct tttgttccgg gcgcacctcg gcaaaaacat
     3781 tttgtcccgg tattcccgcc tgcttcgcaa tggcttgcgc ggtcagcgca ttgtcgcccg
     3841 tcaccagaaa agtttttaaa ccgtcctgct gcaactgccg catcacttca tccgcgcccg
     3901 gtttgagcgt atctttcacc gccagcatcc cgagcaatgt tttgtcagcg gcgaacccca
     3961 acaacgtcgc gccttgaccc gaccattttt caacaaatgc cttggctggc ttcaaatcca
     4021 cgcccatttc ctgcaaccat tgcaatgagc ccaaacgcaa aattccatat tccgcactcc
     4081 gcactccgcg ttccgcactc attccgccct ggacccccgc tccgcgcact tcctcaaaat
     4141 cttctaacgg aaattcttcg ccatgcagct tcgccacggc ctggctgagt ggatgattcg
     4201 accgtcgcgc caaggcggac gccaatttca tggcatccgg cgcggatgca taaacttcct
     4261 gggccgccac gtccggcttg ccttgcgtca acgtgccggt cttgtcgaaa acgattgcgg
     4321 tgatccgacc cgcgcgctcc aacgccacac cgtcccgcac cagaattccc cggcgcgccg
     4381 ccgcattcgc tcccgccatg atcgccactg gcgtcgcgag tcccatcgcg caaggacacg
     4441 cgatgatcaa caccgccgcc gactggatga acgccgccgc caatgcgctt gcgggcagga
     4501 ctgcaggcca caaaaatttc gacaaccact cgctcgtctg ccgcgcctct tcgtcggcca
     4561 aaccccacca aagccccgtg cccaacgcga tcaacaccac caccggcaca aagacgctgc
     4621 tcacgcggtc gcctagacgc tggatttccg cgcggctgtt ttgcgcccgc gcgaccgccg
     4681 cgatgatgtg cgccaatgcc gtttcttcac cagtccccgt gacgcgcatg agaatccgcc
     4741 cgttcaagtt caccgtgcca gcgtataatt tttcgcccgg ggttttttca acgggcagcg
     4801 attcgcctgt gagcatcgat tcatccaccg cgcccgcgcc ttcggtgact tcgccgtcca
     4861 ccggcacatg atcgcccggc ttcaagacca ccgtgtcggt caactgcaat tcgcgcaccg
     4921 gcacttccgc ttccgagccg tccgggttgc agcggcgcgc catctcaggg gccagatgca
     4981 gcaacgcctg caacgcgctc tccgcccgcg tgctcgtgcg cgcctcaaac caatggccca
     5041 agctgacgat ggtgatgatg gccgccgatt ccatgaaaaa gagatgtccc ggcgtgccgg
     5101 tgaacaacgc ccaaacactg taaacgaatg ccgtcgtcga accgagcgcc accagcgtgt
     5161 ccatattcga cgcgccgacc ttcaactgac gccatgcgcc gacgtaaaat tttgccccgc
     5221 aaaaaatctg aaccggcaat gccagcgcaa acgacaacca tcgaaaccac cgttccatcc
     5281 ccagcccgaa cgcccattcg ccgataatca acggcaacgt gcagatcagg ccgaccacca
     5341 cgttgaaccg ccaacccgcg agcggcgacc atttcgtttt cgcttcggca gcgaccgttt
     5401 gttcgtcaag cggtttggct tcgtaacccg cctcggaaac ggcccgcaac acctccggca
     5461 ggttcgaacc atccttccag cgcacggttg cgcgcccggc attcaattgc accgaggcgc
     5521 ttgccacacc cgacacgctt tggatggcct cggcgacgtg ccgcgcgcaa ttgttgcagg
     5581 tcatccccgt gaccagcaaa tccgtcgcgt gtggcgaggt tgtttcagga tggttcataa
     5641 aagatgacag tccgctccga caccttaacc gaaagggaaa aaaagtcgaa cagcacaaag
     5701 cggcat
//
LOCUS       DASZMM010000343         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1264228, whole genome shotgun sequence.
ACCESSION   DASZMM010000343 DASZMM010000000
VERSION     DASZMM010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264228"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1118
                     /locus_tag="VG754_08335"
     CDS             258..1118
                     /locus_tag="VG754_08335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWF19261.1"
                     /translation="MRLDRFLTLHLFQPWQWSAGAGELPVLMYHSISDTKEQGPAYYH
                     TATSPKLFSEQMALLRANGFRAVTLRQGRELLKRDGKAGKAVAITFDDGSRDFYADAF
                     PVLQQHGFSATMFLPTAFIREKRRMFKERECMTWTEARELHRAGIEFGSHTINHPKLY
                     ELDFSDIRTELQVSKWVIEDKLGVAISSFAYPYAFPSADRDFVETLTDLLKGSGYECN
                     ATTRIGRVHAEDDPYTLKRLPVNSGDDSALFLAKIRGAYDWMNWPQDRLKKLKVAKNG
                     RTQQRLCRAP"
     gene            1128..2009
                     /locus_tag="VG754_08340"
     CDS             1128..2009
                     /locus_tag="VG754_08340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF19262.1"
                     /translation="MFSRRYIIISPVRNEEAHLRETIESVASQTVRPIRWIIVNDGSN
                     DKTPQIADEAARAHGWITVVHRADRGFRRQGGGVMEAFYDGHKLIGGEPWDFLIKMDG
                     DLSFAPDYFEKCLAKFEEDAKLGIGGGTICVTGDGGLRVESKGDPAFHVRGATKIYRR
                     GCWEAIGGLLRETGWDTLDEVKANMLGWRTFTFPDLQLVHHRETGGADGTWKNWVKNG
                     RANYVVGYHPLFMMCKCARRIFERPYLIVGVGLFYGYLTGYILRAPCNREPDVVRYLR
                     RQQLRRLFLQSSIWADK"
     gene            2033..3922
                     /gene="asnB"
                     /locus_tag="VG754_08345"
     CDS             2033..3922
                     /gene="asnB"
                     /locus_tag="VG754_08345"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWF19263.1"
                     /translation="MCGIAGKLVFDASAKVETADIQAMLGPMSHRGPDGQGIHLDRNV
                     GLGHLRLSIIDLSTGAQPMTNEDKTVWVVFNGEIYNFQELRKRLLAKGHVFQSHSDTE
                     VIIHLYEDLGPDCVRELRGMFAFAIWDAKQQRLFVARDRVGIKPLYYCQTDKALYFAS
                     ELKGIIADRSVSREINLSALRQFFCFNYVPGDETLFKSVRKLLPGHYLTVENGRAFQR
                     EYWDLRFTQNRWHMPWEKAVDELHGLLRTVVKDHMIADVPVGVLLSGGVDSSAILSFA
                     VGATDKKVQTFTVGFDGKEVVDERPYAKLSAQKYGTEHHEISITSEDFWNFLPSYVWH
                     MEEPVCEPPAVALYYVSKLARGHVKVLLSGEGGDEAFAGYPNYPNMLRLDQIRAAAGP
                     LARTAGVAATIAGKVTREKRLMRYGAALGRPLSSQYFSRTSGPTAFFNRHAQKFFSAE
                     FEELSSSVSPAGFISGLTQVVNEEPLLNQMLYVDTKTWLPDDLLVKADKITMANSVEL
                     RVPLLDHQVLEFAASLPPDFKVRGKETKRILKAAFAEELPAEVLNRKKAGFPVPYEMW
                     LRRDLKREVEDVLLSESANKRGYFRKSEIERLLKANARDGSYSKEVFSLLAVELWHRK
                     FVDEN"
     gene            3967..>4350
                     /locus_tag="VG754_08350"
     CDS             3967..>4350
                     /locus_tag="VG754_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19264.1"
                     /translation="MESNSIYTDFRKGSDLAQSDPVRVLYSFPLKIGAARICTTAWHQ
                     AQGLADAGAQVTVFPAAICRDFSPAVKVRPTLAWGKFRLPNRLMGRMGYAAMHDRMVA
                     ARLAKLAGKIDIIHTWPLGARETLKT"
BASE COUNT          932 a         1106 c         1346 g          966 t
ORIGIN      
        1 gcggcgttgg cggacgcgat ggtggaagtg ctgggcaacg cagaattgcg gcgcggcatg
       61 gtcgcgcgcg cgtccgaata cgcgatgcac aacagttggg aggtgcgcaa ggccgattat
      121 ctgcaactgg tggattcgct ctgttcagtg agccgcaagt gatgatgaga ggatagaaga
      181 tagaggatgg gggatgtgaa gaccggctct gcctgatggt ccgtcctcga tcttcgttct
      241 tttatcctca gttttttatg cgattggacc gttttttgac tttgcacctg tttcagccgt
      301 ggcaatggtc ggcaggcgcc ggtgaattgc cggtgctgat gtatcacagc atctcggaca
      361 cgaaagagca gggcccggct tattaccaca ccgcgacaag cccgaaactt ttttccgaac
      421 aaatggcgct gctgcgggcc aatgggtttc gagcggtgac gctacggcaa ggacgcgaat
      481 tgctgaaacg ggacgggaag gcggggaaag ctgtcgcgat cacattcgac gatggttctc
      541 gcgattttta cgcggacgct tttcccgtcc tgcaacaaca tgggttcagc gccacgatgt
      601 tcctgccaac ggcgttcatc cgcgagaagc ggcgaatgtt caaggaacgc gaatgcatga
      661 cgtggaccga agcgcgggag ttgcatcggg ccggaattga gttcggttcg cacaccatca
      721 atcatcccaa gctttatgaa ctggatttca gcgacattcg caccgagctc caggtttcca
      781 aatgggtgat tgaggacaaa ctgggcgtgg cgatttcatc atttgcatat ccttatgctt
      841 ttcccagcgc ggaccgcgac tttgttgaaa cgctgaccga cctgttgaaa ggttcgggtt
      901 acgagtgtaa tgcgaccaca cgcatcggcc gcgtgcatgc cgaagatgat ccgtacacgt
      961 tgaaacggtt gccggtcaat tccggcgatg acagcgcatt gtttctcgca aaaattcgcg
     1021 gcgcatatga ctggatgaac tggccgcagg accgattgaa gaaattgaaa gtcgctaaaa
     1081 acggacggac acaacagcgc ctttgccgcg cgccctgatt tctggccatg ttttcacgcc
     1141 gttacatcat catctcgcct gtgcgcaacg aagaagcgca cctgcgtgaa accatcgagt
     1201 cggttgcgtc acaaactgtc cgccccatcc gttggatcat cgtcaatgat gggtcgaacg
     1261 acaaaacgcc gcaaatcgcc gatgaagcgg cgcgcgcgca tggctggatc accgtggtgc
     1321 atcgtgcgga ccgcggtttc cggcgacagg gcgggggagt gatggaagcg ttttacgacg
     1381 gtcacaaact tatcggcggc gagccgtggg attttttgat caagatggat ggcgaccttt
     1441 cgtttgcccc ggattatttt gaaaagtgcc tcgccaaatt cgaagaggac gccaagctgg
     1501 gcatcggcgg cgggacgatt tgcgtgacag gcgacggtgg cttgcgcgtg gaatcgaagg
     1561 gtgatccggc atttcatgtg cgcggcgcga cgaaaattta ccggcgcggc tgctgggaag
     1621 cgattggcgg actgttgcgc gaaacaggct gggacacctt ggacgaagtg aaggcgaaca
     1681 tgctcggctg gcgcacattt acgttccccg atttgcaatt ggtgcatcat cgcgaaaccg
     1741 gcggcgcgga cggcacctgg aaaaattggg tgaagaacgg gcgcgcgaat tacgtggttg
     1801 gttaccatcc gttgttcatg atgtgcaaat gcgcgcggcg cattttcgag cggccttatt
     1861 tgatcgtggg cgtcgggctt ttctacgggt atctcacggg atatatattg cgcgctccct
     1921 gcaaccggga gccggacgtg gtccgttact tgcgccgcca gcaattgcgc cgcttgttcc
     1981 tccagagcag catctgggct gacaaataga aatcaattcg cgattaattt ccatgtgcgg
     2041 catagctggc aaattggttt ttgatgcttc ggcgaaggtc gaaaccgccg acatccaggc
     2101 gatgcttggc ccgatgtccc atcgcgggcc ggacgggcag ggaatccacc ttgaccgcaa
     2161 cgtcggcctt gggcatctcc gtttgtccat cattgatctg agcaccggcg cgcaaccgat
     2221 gaccaatgaa gacaagaccg tttgggtcgt gttcaacggc gagatataca atttccaaga
     2281 gttgcgcaaa cggctcctgg ccaagggcca tgtttttcaa tcgcattcag acaccgaagt
     2341 gattattcat ctttacgaag atctcgggcc ggattgcgtg cgggaactgc gcggaatgtt
     2401 tgcgttcgcg atttgggacg cgaagcagca acggcttttt gttgcgcggg accgtgttgg
     2461 catcaagccg ctctattatt gccagaccga caaggcgctt tacttcgcgt ccgaacttaa
     2521 aggcatcatc gctgaccgtt cggtcagccg ggagatcaat ctttcagcgt tgcggcaatt
     2581 tttctgtttc aattatgtgc ccggcgacga aacgttgttc aaaagcgtcc gcaaacttct
     2641 gccgggtcat tacctgacag tggagaacgg acgcgctttt caacgcgaat attgggactt
     2701 gcgtttcacg cagaaccgct ggcacatgcc ctgggagaaa gcagtggatg aattgcacgg
     2761 cctgctccgg acggtggtga aggaccacat gattgccgat gtgccggtgg gcgtattgtt
     2821 gagcggcggg gtggattcat ccgcgatcct cagttttgct gtcggggcaa ccgacaaaaa
     2881 agtccagaca ttcacggtcg gtttcgacgg caaggaagtg gtggacgagc ggccttacgc
     2941 gaagctttcg gcgcaaaaat acgggactga acatcacgaa atcagcatca cgtcggaaga
     3001 tttttggaat tttttgccgt cgtatgtctg gcacatggaa gagcccgtgt gcgaaccgcc
     3061 ggccgtcgcg ctttattacg tttcaaagct ggcgcgcggg catgtgaagg tgttgctttc
     3121 gggcgagggc ggtgacgaag cttttgcggg ttatccgaat tacccgaaca tgttgcggct
     3181 tgatcagatt cgcgcggcgg cgggtccgct ggccaggact gccggagtcg cggcgacgat
     3241 tgccggaaag gtgacgcggg aaaagcgctt gatgcgttat ggcgcggctt tgggccgtcc
     3301 gctttcgtcg caatatttca gccgcacatc gggaccgact gcgtttttca accggcatgc
     3361 gcaaaaattc ttcagcgcag aatttgagga acttagctct tcggtttcgc ccgccggatt
     3421 catcagcgga ctgacgcaag tggtgaatga agagccgttg ctcaaccaaa tgttgtacgt
     3481 ggatacgaaa acttggctgc ccgatgattt gctcgtgaaa gccgacaaga tcacgatggc
     3541 caattccgtg gaattgcgcg tgccattgct cgaccatcaa gtgctcgaat tcgcggcatc
     3601 gctgccgccg gatttcaagg tgcgcggcaa ggaaaccaag cgcatcttga aggccgcgtt
     3661 tgccgaggaa ctgcccgcgg aagttttgaa ccgaaagaag gccggctttc ctgtgccgta
     3721 tgagatgtgg ttgcggcgcg atttgaaaag ggaagtcgaa gacgtgctgc tttccgaaag
     3781 cgcaaacaag cgcggatatt ttcgcaagag cgagatcgaa cggctgctga aggcgaatgc
     3841 gcgcgacggc tcctattcga aggaagtttt ttcattgctg gccgtggaat tgtggcatcg
     3901 caagttcgtg gacgaaaatt gagcagcccg cgcttcccgg tttgaaactt tgaaaacgtg
     3961 aacacgatgg aatccaacag catctacaca gatttccgga aaggcagcga cttggcgcaa
     4021 agcgatccgg tccgggtgct gtacagtttt ccgctgaaga tcggcgcggc gaggatctgc
     4081 accactgcat ggcatcaggc gcagggactg gctgacgccg gggcgcaggt gacggttttt
     4141 ccggcggcga tttgccggga cttctctcct gcagtcaaag tgcggccgac actcgcgtgg
     4201 gggaagttcc ggttgccgaa ccgcttgatg gggcgcatgg gatatgcggc gatgcacgac
     4261 cgcatggtgg cggcgcggtt ggcgaagctt gccggcaaga ttgatatcat ccacacttgg
     4321 ccgttaggcg cgcgggaaac gctcaagacc
//
LOCUS       DASZMM010000344         4998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2344228, whole genome shotgun sequence.
ACCESSION   DASZMM010000344 DASZMM010000000
VERSION     DASZMM010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344228"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..537
                     /locus_tag="VG754_08355"
     CDS             196..537
                     /locus_tag="VG754_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19265.1"
                     /translation="MPEEMRNYVGLNGASSSKDKLFACPADAFYPNHVLTNAWPLYYV
                     RKSLHEEAILDFSSYSFNGGATNSTIRFPGITGLKLSAIKHPARTGVGYGSFGDWPMV
                     MASAGTSFAFR"
     gene            548..685
                     /locus_tag="VG754_08360"
     CDS             548..685
                     /locus_tag="VG754_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19266.1"
                     /translation="MISFVDGHVNYIKIYWDSTPLPSGSLSFAMSYNPLTGYDYQWSG
                     D"
     gene            918..1178
                     /locus_tag="VG754_08365"
     CDS             918..1178
                     /locus_tag="VG754_08365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWF19267.1"
                     /translation="MNAELCKKALEKVGNPNVLINLVSRRVRQLNSGGGAASRPLIAD
                     VGNMGAADIALAEIIEEKMGFHVPELAETEKPSNNSKKRKKH"
     gene            1279..1734
                     /gene="smpB"
                     /locus_tag="VG754_08370"
     CDS             1279..1734
                     /gene="smpB"
                     /locus_tag="VG754_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWF19268.1"
                     /translation="MADILNNPKARRDYHILETFEAGIVLHGTEVKALRAGKGQIADA
                     FARVEKDEVFLHNAHIDEYNFGNRQNHQPKAVRKLLLHKPEIRKLFALSAVKGNALFP
                     LSFYWKNGRVKVALAVGKGKVQFDKREDLKKRDSDRELKRATMLRSKGK"
     gene            complement(1854..2126)
                     /gene="rpmB"
                     /locus_tag="VG754_08375"
     CDS             complement(1854..2126)
                     /gene="rpmB"
                     /locus_tag="VG754_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWF19269.1"
                     /translation="MSKFCEITGTGPMKGSHIWRSGKAKKKGGIGTHVTAITKRRFMP
                     NLQRVKALINGEVRYIRVTANALKKGMVVKAPKRNWKKPEGAAAKA"
     gene            2338..3237
                     /locus_tag="VG754_08380"
     CDS             2338..3237
                     /locus_tag="VG754_08380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19270.1"
                     /translation="MGSPNIAKVGEPFVSIILRSFNEAWALRDTLPAVQAQEHRNWEL
                     IVIDSMSTDGSVELIRKARPQHFVQIEKYNPSRVMNLGMQLARSEFGIFLNADATPQG
                     TNWLRPLLQPLLNPKNAAVFGRQIPRPDCEAVYACDYERCFGPRRESDKWEHFFSMVS
                     SGLRKDIWSKRGFLEKMQYSEDDEYTRWCRSQGYEVVYCPESVVTHSHNYSPQQAHKR
                     SFGEGKALAAVWTGRPTEINWLRTVMLGSIRDALRDLKFCAAHRRMREFPHAMRIRCR
                     QRAAKLAGFREGWAYYRQSPIKT"
     gene            3306..4508
                     /locus_tag="VG754_08385"
     CDS             3306..4508
                     /locus_tag="VG754_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19271.1"
                     /translation="MSKSSEMTAPEREHGSTGSKTRFTISGTESLETRLADMCQSVLI
                     GVQQIIPAGRLQLLVLGGGYGRGQGGVLRTDEGDKPYNDLEFYVFVRGNLFLAERRHR
                     VALQELGEHLSPEMGLHVEFKLYSLEKLRRGQISMYSYDLVAGHKIIYGGEKLFRGCE
                     HHLAPEQIPASEATRLLFNRCSGLLLVRNLLRSPNLNPEQSDFIGRNLAKAQLGFGDA
                     ILTLFGLYHWDCRERHERLNTLKPFDPPSWLSQVRHHHAEGVLFKLHPQRATNSRDEL
                     GSQFAELHELGRRIWLWIEGRRLNRVFKSARDYSLNDLKKCPDSTLVRNCLLNLKTYG
                     PRAPFTGGFSRYPREKLFNALTILLWDDNVPHERAVLQRLQNQLRARSTRWEDLISAY
                     MRLWQTYG"
     gene            4501..>4998
                     /locus_tag="VG754_08390"
     CDS             4501..>4998
                     /locus_tag="VG754_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HWF19272.1"
                     /translation="MAETLHIDWPTPPAGFSLGAHDVHVWAATLLLPQERAAIYESSL
                     STDERTRTARFMFERDRRRFVAGRGILRAILSNYIQRQPAQIRFDYSERGKPSLAGLS
                     SNEQIHFNLAHSEDLAVVAVSRIPGIGVDVERLRPMNDAEGIAERFFSARESKLLKSL
                     PSDQKL"
BASE COUNT         1204 a         1298 c         1326 g         1170 t
ORIGIN      
        1 aaaggttctt caaaagtgac ttaagtttcc aggagccaat tggcggctgc gaagcacgca
       61 gtatccgtac gttagagaga ggcggataca aaaattcgat ttgaattcgg gctttatctg
      121 ccataatttg tctgcaccgg aaggatttcc aagatgatca caaaagtcac agtgactggc
      181 gtggatgatt aacagatgcc cgaagaaatg agaaattacg tcgggctgaa cggtgcgtcc
      241 tcatcgaagg ataaattatt tgcctgtcct gcggacgcgt tttatccgaa tcatgtcttg
      301 accaacgctt ggccgttgta ttacgtgcga aagagtttgc atgaggaagc cattttagat
      361 ttctcgagct attcattcaa cggcggcgcc acaaattcta caatcagatt tccaggcatc
      421 accggcctca aattaagcgc aatcaaacat ccggccagaa cgggcgttgg ttatggaagc
      481 ttcggcgatt ggcccatggt catggcatca gccggcacct cgtttgcttt tcgctgacgc
      541 caaaaacatg atcagtttcg tggacggtca cgtcaactac atcaaaattt actgggacag
      601 cacaccgctt ccaagcggaa gcctttcatt tgcgatgagc tacaatccgc tcaccggata
      661 cgattaccaa tggagcggcg attgaaaaac tagtttctaa acccttgcca agcttactat
      721 aacgatatag ttgattcttt tcaactgttt tagccgcttt actccgaaaa aactggggtg
      781 tatttgagtt ccattcgcat aaagctgtgc attatgtatg gttatttaat tcgctttttt
      841 gcggaggtat tattctgttg ctttgatccg ttaaagaagt aaactaattg gctcgtcttt
      901 taattggaca aaaacatttg aacgctgaac tttgcaaaaa agcattggag aaagttggca
      961 accccaacgt tctgataaac ctcgtctccc ggcgcgtgcg ccagctcaat tccggcggcg
     1021 gagcagccag ccgcccactg atcgccgatg tgggaaacat gggcgctgca gacatcgccc
     1081 tggcggaaat catcgaagag aaaatgggct ttcatgtgcc tgagttggct gagacggaaa
     1141 agccgtccaa caacagcaag aagcgcaaga agcattaggg gaattggagt attggagtgc
     1201 tggagtaaag tgagggaata ggtttttgcc attccatcat ttcatctctc cgatactcca
     1261 cgactccaat gtccctctat ggcggacatc ctcaacaatc ccaaagcgcg ccgcgattac
     1321 catattctgg aaacgttcga ggcgggcatc gtcctgcacg gcacggaggt gaaagccttg
     1381 cgcgccggca aaggacaaat cgctgatgct ttcgcccgcg tggaaaagga cgaggttttc
     1441 ctgcacaacg cgcacattga cgaatataat tttggaaacc gccaaaatca tcagccaaaa
     1501 gccgtccgca agctgctgct gcacaaaccg gagatccgca aattatttgc cttgagcgcg
     1561 gtgaaaggaa acgcgctgtt tcctctgtcc ttctattgga aaaacggccg ggtgaaggtg
     1621 gcgttggccg tcggcaaagg gaaggtgcaa ttcgacaagc gcgaagattt gaagaagcgc
     1681 gattcggacc gcgaactcaa acgcgccacg atgctgcgat caaagggaaa gtgaattcag
     1741 cccccggatg cacacaggta agcacggatt ctgccgccat gaaatccgat taattcgtaa
     1801 ataaaaaaag cgaagccttt ggcttcgctt ctacatggaa ctgtgaagac aaatcaggct
     1861 tttgctgccg cgccttcggg cttcttccaa ttgcgcttgg gcgctttcac aaccatgcct
     1921 ttcttgaggg cgttggcggt gactcgaatg tagcgcactt cgccattaat caatgccttg
     1981 acgcgctgga gattgggcat gaaacggcgc ttggtgatcg cggtcacgtg cgtgccgatg
     2041 ccgccttttt tcttggcttt accactgcgc catatatggc tgcccttcat ggggccggtg
     2101 ccggttattt cacaaaactt tgacataaat ttctttgcct ttcaaaagga acggaaaagg
     2161 taccaatctg aggccgtgtt gcaagccttt tttcgggctt tttgcaaacc ggctttcccg
     2221 caactgccat gggcaaaatg ccctatcatc aaacaggggg caacttggat atattgtccc
     2281 cgaaattcgg acgacaacat ccgccacgat ggaggtggcg tcaagaacgt caagaacatg
     2341 ggttcaccta atattgcaaa agtcggcgag ccattcgtca gcatcatcct gcgctcattc
     2401 aacgaggctt gggcgttgcg ggacacgctg ccggcggttc aggcgcagga acatcggaac
     2461 tgggaattga ttgtcattga ttccatgtca accgatggct ccgtggaact catccgcaag
     2521 gcgcgaccgc agcattttgt tcaaatcgag aaatataatc ccagccgcgt gatgaatttg
     2581 gggatgcagc ttgcccgctc ggaatttggc attttcctca acgccgatgc cacgccgcag
     2641 ggaaccaact ggctgcgacc tcttttgcag ccgttgctca atccaaaaaa cgcggcggtt
     2701 tttggacggc aaattccaag accggattgc gaggcggtct atgcatgcga ttacgaacgg
     2761 tgttttgggc cccgccgaga atcggacaag tgggaacatt ttttcagcat ggtcagcagc
     2821 ggcttgcgca aggacatctg gtcgaaacgc ggcttccttg aaaaaatgca atattcggaa
     2881 gacgacgaat acacccgttg gtgccgctcg caaggttacg aggtggtcta ttgcccggag
     2941 tcggtggtga cgcattcgca taattacagt ccgcaacagg cgcacaagcg cagcttcggc
     3001 gaaggcaagg cgctcgctgc ggtttggaca ggacgaccaa ccgaaatcaa ctggctgcgc
     3061 accgtgatgc tcggctcgat tcgcgatgca ttgcgagact tgaaattttg cgcggcgcac
     3121 cggcgcatgc gggaatttcc gcacgccatg cgcatccgct gccgccaacg cgcggcgaaa
     3181 ctggccgggt tccgcgaagg ctgggcttat taccgccaat cccccatcaa aacgtgatcg
     3241 ccacaacaac tcagaaacaa ccggagagcc cgcacggaac gcaaatttca gatttgaaat
     3301 ctgagatgtc gaagtcgagc gaaatgactg cgccggaacg cgagcatggg agcacaggtt
     3361 caaaaacgcg tttcacaatc agcggcacgg aatcattgga aacgcgtttg gccgatatgt
     3421 gccagagtgt gttaattggc gtgcagcaaa ttattcccgc cgggcgattg caactgctgg
     3481 ttttgggcgg tggttatggt cggggccagg gcggcgtgtt gagaactgac gaaggcgaca
     3541 aaccttacaa cgatttggaa ttttacgtgt tcgtgcgtgg aaaccttttc ctagccgaac
     3601 ggcgtcatcg cgtcgctttg caggaattgg gcgagcatct ttcgccggaa atgggattgc
     3661 acgtggagtt caagctttat tccctcgaaa aactgcgtcg cggccaaatc agcatgtaca
     3721 gttatgacct ggtcgcgggt cataaaatca tttacggcgg tgaaaaattg tttcgaggat
     3781 gcgaacatca tcttgccccc gaacaaattc cggccagcga agccacgcgc ctgctcttta
     3841 atcggtgctc cgggttgttg ctcgtccgca atctgctgcg ttcgccgaat ctcaatcccg
     3901 aacagtcaga tttcatcggc cgcaacctcg ccaaggcgca acttggcttc ggcgatgcca
     3961 ttctgacgct gttcggtctt taccattggg actgtcgtga acggcacgaa cgcctgaaca
     4021 cgctcaaacc gttcgacccg ccatcttggc tttcgcaagt gcgccatcat cacgccgaag
     4081 gcgttttatt caagttgcat ccgcagcgcg cgaccaattc tcgtgacgaa ctgggatcgc
     4141 aattcgccga acttcatgaa ctcggccgcc gcatctggct ctggatcgaa ggccgtcggt
     4201 tgaatcgcgt ttttaaatct gcgcgtgatt attccctgaa tgatttgaag aaatgtcccg
     4261 attcgacgct cgtccgcaac tgcttgctga acctgaaaac gtacggcccg cgagcgccat
     4321 tcaccggtgg attttcccga tatccacggg aaaagctttt taacgcgctt accattttgc
     4381 tttgggatga caacgttcca cacgagcgcg cggtgttgca aaggctgcaa aaccaattac
     4441 gcgcaagatc aacgcgttgg gaggatttga tttcggccta catgcgactt tggcaaactt
     4501 atggctgaaa cattgcacat tgattggccc acgccgcccg ccgggttttc gctcggcgcg
     4561 catgatgtac atgtctgggc tgccactctt ctgttgccgc aagaacgcgc ggcgatttat
     4621 gagtcgagcc tttcgaccga tgagcggacg cgcaccgcgc gtttcatgtt cgagcgtgat
     4681 cgccgccgct ttgtcgccgg gcgcggaatc ttgagagcca tcttgagcaa ctacattcaa
     4741 cggcagccgg cgcaaatccg attcgattac agcgaacgcg gcaaaccttc gctggctggt
     4801 ttgtcttcga acgaacagat tcattttaat ctcgcgcact cggaagacct ggcggttgtc
     4861 gctgtgagcc ggattccggg aatcggcgtc gatgtcgaga gactgcgccc gatgaacgac
     4921 gccgaaggca tcgcggaacg ctttttttca gcgcgtgaaa gcaagcttct caaatcattg
     4981 ccttcggacc agaaactg
//
LOCUS       DASZMM010000345         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_955285, whole genome shotgun sequence.
ACCESSION   DASZMM010000345 DASZMM010000000
VERSION     DASZMM010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955285"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VG754_08395"
     CDS             complement(<1..345)
                     /locus_tag="VG754_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19273.1"
                     /translation="MESILPFSGRRAVVLGGLALLACLCLAGNLARAQNTPTASANPT
                     SDAANAEGDKAWKETKKLTQPPMPPAAWQQQQPSREEVAKFYTDALMKAANAAKDFYT
                     KFPNHPKAEEARQ"
     gene            complement(360..1001)
                     /locus_tag="VG754_08400"
     CDS             complement(360..1001)
                     /locus_tag="VG754_08400"
                     /inference="COORDINATES: protein motif:HMM:NF037718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HWF19274.1"
                     /translation="MSQIQNPALKNFAAQIGDELVFAQVLIKRQGNGHELRHIEDRET
                     AAISLRAIKPEDARTLAQYTANGEFRPLKSAPNLQRGWRIVAVNDTELEIALSRLYPG
                     AVADWHAAQSPSPPVTHYREYTERQSGMYRITTMLTDAQVAQVVGEVCDVKHCLKRRF
                     WSVPGLQPDAAENKSLIPCLEPCAVLMESARKAVRADQKEKLAMTLAEENKAG"
     gene            complement(1253..1462)
                     /gene="thiS"
                     /locus_tag="VG754_08405"
     CDS             complement(1253..1462)
                     /gene="thiS"
                     /locus_tag="VG754_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWF19275.1"
                     /translation="MKQNVIIANGTKIEGEFPCSLEDFLKRQNLPPRSVVVEHNGEAV
                     APSEFTRRTLAAGDRLEIVKIVAGG"
     gene            complement(1470..2195)
                     /locus_tag="VG754_08410"
     CDS             complement(1470..2195)
                     /locus_tag="VG754_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HWF19276.1"
                     /translation="MKVSNIGGIIRRRILVNYRVAPDVIQPLLPPKFLPKLHDGSAIA
                     GVCLIRLEQVRPKGIPSFLGMSSENAAHRVAVVWQDEHGKPQEGVYIPRRDTNSRFNQ
                     AVGGRFFPGEHHAAKFIVNDDRDRIDFEMISEDQSVLVRVRGKNSSELSRDSGFKNIA
                     EASAFFEPGSLGYSATRKGDRLEGLRLRTAQWKISPLQVEHAHSSFFDDKTKFPPGSV
                     AFDCALIMRDIEHEWQSESDLYI"
     gene            complement(2526..3053)
                     /locus_tag="VG754_08415"
     CDS             complement(2526..3053)
                     /locus_tag="VG754_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19277.1"
                     /translation="MTLQQKGRFRKRWLLIPLMGIGLLLTILFIESNSPPISDEAFKE
                     QFKKAAPEMKLQVVKETGGWLNWMDIPVMTRLSMSHLTPRGPFIEMINTKDGTTAFFA
                     VDDETKTNFEIDVHFKDHQVRAVAVHYPKDGESRAGQIRNELEKRFNGLPVVLRKEFL
                     FYPQVLSASNTNSNN"
     gene            complement(3060..>3437)
                     /locus_tag="VG754_08420"
     CDS             complement(3060..>3437)
                     /locus_tag="VG754_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HWF19278.1"
                     /translation="RELAQLICAFRLFLPDAGLVLSTREPAKLRDGLIPLGVTLISAG
                     SHTEPGGYTGAGREKIHHTERGRIVELAAGASEWATSNGNATNATGQFDIADERSPEE
                     MAGLIRRLGYEPVWKDWDAALTA"
BASE COUNT          748 a          968 c          883 g          838 t
ORIGIN      
        1 ctggcgcgcc tcctcggcct tgggatggtt gggaaacttc gtgtaaaaat cctttgccgc
       61 attcgccgcc ttcatgagcg cgtccgtgta aaactttgcg acctcctcgc ggcttggctg
      121 ttgctgctgc cacgcggccg gcggcatggg cggttgggtc agctttttcg tctctttcca
      181 cgccttgtca ccttccgcgt tcgcggcgtc cgaagtggga ttggcgctgg cagtgggcgt
      241 gttctgggcg cgcgccagat ttcctgcaag gcacagacag gccagcaacg ccaacccgcc
      301 gagtacaacc gcgcggcgac cggaaaatgg gaggatagat tccatagata aactgttctt
      361 taacccgctt tgttctcctc tgcaagcgtc attgcgagct tttctttttg gtcggcgcgg
      421 acggctttgc gggccgattc catgagcacc gcgcaaggtt ccagacaggg aatgagactc
      481 ttgttttcgg cggcatccgg ctgcaatccc ggcacggacc agaaccttcg tttcaaacaa
      541 tgcttcacgt cgcaaacttc gccaacgacc tgcgcgacct gcgcatcggt caacatcgtc
      601 gtgatgcgat acatccccga ctggcgctcc gtgtattcgc gataatgcgt cactggcgga
      661 gacggacttt gcgccgcgtg ccaatccgcc accgcgccag gataaagacg cgacaaggca
      721 atttccagtt cggtgtcatt caccgcaacg atgcgccagc cgcgttgcaa attgggcgcg
      781 gatttcaacg gacgaaactc gccattcgcg gtgtattggg ccagcgtgcg ggcgtcttcg
      841 ggtttgattg cgcgcaagga gattgccgcc gtttcgcggt cctcgatgtg gcgcagttcg
      901 tgaccgtttc cctgacgctt gatcaacacc tgtgcgaaaa ccagttcgtc accaatttgc
      961 gcggcgaagt ttttcaaagc tggattttgg atttgggaca tcggggtgaa tttacgattt
     1021 acgacatgcg atttacgagg ggttcgtgtt gcaggagccg acgtgaggag gccctccaat
     1081 tgatttcaaa tctgaaattt gaaatttcac attcacccgc gtcattacct cgtctcctgc
     1141 caggaaggac atttctgaat gaattgccaa ggcgcgcacg acgagcaaca cgcatcacgc
     1201 aacacatgcg cgcgtaaatc gtaaatcgca aatcgtaaat cataaaatca tctcatcctc
     1261 ccgccacaat tttcacaatc tccagccggt ctccggccgc caaagtcctc cgcgtaaatt
     1321 cagaaggcgc gactgcttcg ccgttgtgct cgaccaccac gctgcgcggc ggcagatttt
     1381 gccgcttcag aaaatcttcc aaactgcacg ggaattcgcc ttcgatttta gtgccattgg
     1441 cgatgatgac gttttgtttc atgcgccgtt cagatgtata aatccgattc actctgccat
     1501 tcatgttcga tgtcgcgcat gatgagggcg caatcgaatg caaccgaacc gggaggaaat
     1561 ttcgtcttgt cgtcgaagaa actcgaatgg gcatgttcga cttgcagggg tgaaattttc
     1621 cattgcgccg tccgcaagcg caacccttcc aaccgatccc ctttgcgtgt cgccgaataa
     1681 ccgagcgaac ccggttcaaa aaacgccgaa gcttcggcga tatttttgaa tccagagtca
     1741 cggctcaatt cggaagaatt ttttccacgg acccgtacca gaaccgattg gtcttctgaa
     1801 atcatctcaa aatcgatccg gtcgcgatcg tcgttcacga taaacttcgc cgcgtgatgt
     1861 tcgccgggaa agaaccgtcc gccgacagcc tggttgaacc gcgaattggt gtcgcggcgc
     1921 gggatgtaaa cgccttcctg cggtttgccg tgttcatcct gccaaaccac ggcgacacga
     1981 tgcgccgcat tttcgctgct cattcccaaa aagcttggaa tgccctttgg ccgcacttgt
     2041 tcgagacgga tgaggcagac gccggcaatc gcggagccgt catgcaactt gggcaggaat
     2101 tttggcggca acaacggctg gatcacgtcc ggcgcaaccc ggtaattcac caagatgcgc
     2161 cgccggatga tgccgccgat gttggagact ttcataaagt tcttcgctgg acaaggtatg
     2221 aagagtggct tcgataaatg tgttggaggc tcgccctcac cccggccctc tcccccagga
     2281 gagggtgaat gcattgacct tcaaggagcc tttgattaat tcggttgcaa tcgccgccta
     2341 ttggaagttg ctcagatgga agctgacgac aatgcggcga ttttatttca agccgcggca
     2401 aatggttctc cctctcctcg ggggagaggg cgagcgtaac cactgacatt tcaagcttca
     2461 tcatgacgga cctgcttcta atttccaatt ccactgcaaa cagcgaggaa cctttcataa
     2521 gccctttagt tatttgaatt cgtgtttgat gcggacaaaa cttggggata aaataaaaat
     2581 tccttccgga gcaccactgg caatccattg aatcgcttct ccaattcatt cctgatttga
     2641 cccgctctcg attctccatc ttttgggtaa tgaacagcaa cggcacgaac ttgatgatcc
     2701 ttaaaatgta catcaatttc gaagttcgtt tttgtttcgt catccacagc gaaaaatgcg
     2761 gtggttccgt ccttggtgtt gatcatctca atgaatggac ctcgtggcgt taaatgcgac
     2821 atgcttaatc gcgtcatgac aggaatatcc atccagttta gccagccacc cgtttctttt
     2881 acgacctgca acttcatttc tggcgctgct ttcttgaact gttccttgaa tgcctcatca
     2941 ctaattggcg gagaatttga ttcgataaac aaaattgtta agagcaaccc aatacccatc
     3001 aagggaatca gcaaccaacg ttttcgaaac cggccctttt gttggagagt cataacgatt
     3061 cacgcagtca acgccgcgtc ccaatctttc cagacaggct cgtaacccaa tcggcggatc
     3121 aatccggcca tttcttccgg tgaacgttcg tcggcgatgt cgaactggcc tgtagcgttc
     3181 gtcgcgtttc cgttgctcgt tgcccattcg cttgcgcctg cggcaagttc cacgatgcgt
     3241 ccgcgttcgg tatgatgaat cttctcgcgg cccgcgccgg tataaccgcc cggttcagtg
     3301 tggctgcccg cgctgatgag cgtcactcca agcggaatca atccgtcgcg cagcttggcc
     3361 ggttcgcgcg ttgaaagcac caggccagcg tccggcaaaa aaagccggaa agcgcaaatc
     3421 aactgcgcca gttcgcg
//
LOCUS       DASZMM010000346         5689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_955474, whole genome shotgun sequence.
ACCESSION   DASZMM010000346 DASZMM010000000
VERSION     DASZMM010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955474"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1120
                     /locus_tag="VG754_08425"
     CDS             296..1120
                     /locus_tag="VG754_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWF19279.1"
                     /translation="MTLFRWARRCAVGVVLCVGLSGCFPPADNSSDETKEPHFIAGKN
                     LVEQMDWKGAVDEFEKSLEVNPRSASAHFELGWVCEEKINPPDPAAAIFHYERYLDLS
                     GSQDKADLVKQRINNCKVELVKTVSAVGPSPSGVQRELERVAGENRDLQNQVAQLQAQ
                     IAQLKAAAVLVARTSNPPDASGTPPPSHATVSRTSSGEPQRRETSHAATGTTGSTVSA
                     HVSHSGMTKMHTIKPRETMASIARIYGISLAALEEANPQVRPSHLLAGQTLKVPAP"
     gene            1137..1970
                     /locus_tag="VG754_08430"
     CDS             1137..1970
                     /locus_tag="VG754_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19280.1"
                     /translation="MTSRITFLLVALFWMTMTFLLWRSEYVGHTELGSSVPVELVWRK
                     ILTAPDDSGLEIMHNGKKLGYCGWAANVGQDQAAGKVLSDEVPPEGMVPELTSYRLNL
                     KGNVAVNESGNRLKFDLEIRLTTNQVWQAFDLRLNLRPMVWEVHSVANEQKVRLVVIE
                     GKGRRTEQVFKFSDLQDPQGLMQDLGVPAPLGLLGLMNAGILPKNGGTNTPSLDLGLD
                     WKARNDWVSIGHTSVRAYRLEATLLERYRMRVIVSGVGEILRVDLPDGWELINDQFVN
                     L"
     gene            1982..2734
                     /locus_tag="VG754_08435"
     CDS             1982..2734
                     /locus_tag="VG754_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF19281.1"
                     /translation="MIELVHLTKRFGELVAVDDINLLVPRGQFFAVLGPNAAGKTTTI
                     RILAGLIKPTSGTASVGGFNVQTHPLEARRRLAYVPDFPFLYEKLTPWEFFRFTGQMF
                     QMENGRIQSAAEELVVRFHLEAYVNKPIEGLSHGTRQRVAIVSALLHDPEVLVIDEPM
                     VGLDPQHSRVVKDILKERSKKGMTVFLSRHQLSVAEEMADRIGIIHQGRLVALGTRDD
                     LRRLSGSSGPLEEAFLTLIAEQQEIRPDQKGD"
     gene            3007..4287
                     /locus_tag="VG754_08440"
     CDS             3007..4287
                     /locus_tag="VG754_08440"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWF19282.1"
                     /translation="MKKVIASAGVLALGAIGVQSVRADMDLSTERPWSLSGTLRGFYD
                     DNYNTQPNSSSAHLGSFGFEVRPQVSVAYSSGPTTLNASYTYSERYYVSRPGNKSDQS
                     HDFEFTLLHSFNERYSVDLLESFVDAQEPEVIDSSFSFPLRANGDNYRNTAAANLHGQ
                     ITPLLGFVVGYANSFYDYTSVTPGIDGPSYGTLLNRFEHLVTLDSTWRISEETVGIFG
                     YQFQAVDYIAHGSLAPGAFPYVSPSTRDSYNHMVYVGAQHSVRSDLSVSLKAGVQYSD
                     YYNDTAGSNPSSLGPFVELSGEYTYMDGGTLTVGFRHSKNSTDVGFSTATRTVTDDQE
                     SSSVYATVQQVMTPLSPKLIARLSAQYQYSRFNGGGFNNESDVFYLLGLNLTYQFTHY
                     LSAEAGYNYDLLQSDLPGRGYDRNRVYIGVTATY"
     gene            4353..>5689
                     /locus_tag="VG754_08445"
     CDS             4353..>5689
                     /locus_tag="VG754_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWF19283.1"
                     /translation="MDPLKTSSPPEAKLHFLDYWRIIRIRKTVILAVFLLVVITATIV
                     TFILPEAYSSKAVIKVERDVNDVAGVADHPNMSGVYDPYFIQTEFEVIQSEKVLDKVI
                     DKLDLNTAWQRKYGSDSKFKTSESRQALKRMIELRPERNTMLIDITVYSDNKQEAMQI
                     ANTVAEVYSDYRQEERKKLTLGGIEAFNQVLEVQDQKIKDQEVVLDKLRKDLNIVEMG
                     DPNATGAGASIDPINVQHYNSIKIEAETSYVKEEKVLSKLKDLSPEELRQTIPTAAPD
                     SILGTLLQDLNSAEDQLVTMRSDYGDQHPQVLRLMKLRDRLNHDIDERVSGILKGMEA
                     KVAAAKAVVQYTEDKLYEAKTNDIARAEIGRPYYMAKVDLERLRKFRDVLALRTFQET
                     VDIKMPKTMMVDLIEHAEAGARPVRPNKPLNIFLGIVIGLVVGVGLAFFIEYL"
BASE COUNT         1310 a         1425 c         1644 g         1310 t
ORIGIN      
        1 tcccggtttt tcgaattggc caagctggtt ggtgacgttt gtccatgaac ccatcctcag
       61 ccgttgggcg atccatttca aggtcgttgt tgtttcccgt gcggcgcgca attcgcgcaa
      121 tcaccatgcg agcagatgat aagcggctca actagtataa gtaaatctaa tcgatacaaa
      181 gccctttgta ggctttaggg agggttggcg gacgcatgag ggacgaaaac cctcgaaatt
      241 ttttcaaagc caacggatga ttgcgtgttg ggttggattt gtttagtttt gggtcatgac
      301 tttgttcagg tgggcgcgac gatgcgccgt cggtgttgtg ctttgcgtcg ggttgagcgg
      361 ttgttttccg cccgccgaca attcatcgga tgaaaccaag gagccgcatt tcatagctgg
      421 aaaaaatctg gtggagcaga tggattggaa gggggcggtg gatgaatttg aaaaatcatt
      481 ggaggtgaat ccgcgctcgg cgtcggcgca tttcgaactg ggctgggtgt gcgaggagaa
      541 gatcaatccg cccgacccgg cggcggcaat ctttcattac gagcgatatt tggatttgag
      601 cggcagccag gacaaggcgg acctggtcaa gcaacgcatc aataactgca aggtggaatt
      661 ggtgaagacg gtgtccgcgg tggggccatc accatcaggt gttcaacggg agttggagcg
      721 ggtcgcgggg gagaacaggg atttgcaaaa ccaggtcgcg caattgcagg cgcagattgc
      781 gcagttgaag gccgcggcgg ttttggttgc gcgaacgtcc aatcctcccg atgcttcggg
      841 cacgccgccg ccatctcatg caaccgtttc gagaacgtcc tctggtgaac cgcagagaag
      901 agaaacttcc catgcggcga cgggaaccac aggctcgaca gtttcggcgc atgtgtcgca
      961 ttccggcatg acgaagatgc acactatcaa gccgcgcgag acgatggcgt ccatcgcgcg
     1021 aatttatgga atttcgctgg cggcattgga agaggcgaac ccgcaagtgc ggccttcgca
     1081 tttgctggcg gggcagacgt tgaaggtgcc cgcgccgtga ccggccatat cattgcatga
     1141 cttcgcggat aacatttttg ttggtggcgc tgttctggat gacgatgaca tttctattgt
     1201 ggcgttcgga atatgtcggg cacacggaat tgggcagcag cgtgccggtg gagttggtgt
     1261 ggcggaaaat tctgacggcg ccggatgatt cgggactgga aatcatgcac aacggcaaga
     1321 agctcggtta ttgcggttgg gcggcgaatg tcgggcagga ccaggcggcg gggaaggtgc
     1381 tttcagacga agtgccgccg gaagggatgg tgccggaatt gacaagttac cggttgaact
     1441 tgaaagggaa cgtggctgtg aacgaatcgg gcaaccggct gaagtttgat ttggaaatcc
     1501 ggttgacgac gaaccaggtc tggcaggcat ttgatttgcg cctcaatctg cgcccgatgg
     1561 tttgggaggt gcattcggtg gcgaatgaac agaaggtgag attggtggtc attgaaggca
     1621 agggcaggag gaccgagcag gtcttcaagt tttccgacct gcaggacccg caaggattga
     1681 tgcaggactt gggcgtgccc gcgccattgg ggctgctcgg gttgatgaat gccggaatcc
     1741 tccctaaaaa cggcgggacg aacacgccgt cgctggactt ggggcttgat tggaaagcgc
     1801 gcaacgattg ggtttccatc ggccatactt cggtgcgggc ataccggctg gaggccacgc
     1861 tgctggaacg ctaccggatg cgggtgattg tgagcggggt gggagaaatc ctgcgggtgg
     1921 atttgcccga tggctgggaa ctcatcaatg accaattcgt caatttatag gccggccgcg
     1981 catgattgaa cttgttcacc ttaccaagcg cttcggcgaa ctcgtagccg tcgatgacat
     2041 caatctcctg gtgccgcgcg gccaattttt tgcggtcttg ggcccgaacg ccgccggcaa
     2101 gaccacgacc atcaggattt tggcgggatt gatcaaaccg acctccggca cggccagcgt
     2161 aggcggcttc aacgtgcaga cgcacccgtt ggaggcgcgg cgtcggttgg cgtatgttcc
     2221 ggactttccg tttttgtatg aaaaactgac gccttgggaa tttttccgct tcacgggcca
     2281 gatgttccag atggaaaatg ggcgcataca atctgcggcg gaagaattgg tggtgcgttt
     2341 ccatctggaa gcttatgtga ataagccgat tgaggggctt tcccacggca cgaggcaacg
     2401 ggtcgccatc gtctcagcgc tgttgcatga tccggaagtg ctggtcatag acgagccgat
     2461 ggtggggttg gatccgcagc actcacgggt ggtcaaggac atcttgaagg agcgttcgaa
     2521 gaaggggatg acggtgttcc tttcgaggca tcaattgagc gtggcggagg agatggcgga
     2581 ccggattggc atcatccacc aagggcggtt ggtggctttg ggcacgcgag acgatctgcg
     2641 gcggttaagc ggcagttcgg ggccgctgga agaggcattc ctgacgttga ttgccgagca
     2701 gcaagaaatt cgacccgacc aaaagggcga ttagacgtcc aacgggaatt tttttagctg
     2761 gctttgaacg caaaccggat tccaaagtca atacttccat gccacaccct ttgcaggcca
     2821 cggtcgaggt ttgtcaaagg gagtaagagg cgaagcattt ggcccgaaaa aacagggttt
     2881 caagcatgtc gtgtctcgaa aaaaagatga agatttgttt gacagaaaac gagttcctaa
     2941 agtaaaacac tcctgctttt actgttggaa ctccctcgcg ggccaatatt taaacacgaa
     3001 ccaaatatga agaaagtaat cgcctctgcc ggagtgttgg cgcttggcgc aatcggtgtg
     3061 caaagtgtcc gggccgacat ggacctcagc acggaacggc catggagcct ttcgggaacc
     3121 ctgcgcgggt tttacgatga caactacaac acgcaaccca atagcagctc tgctcacctc
     3181 ggcagttttg gctttgaagt aagaccgcag gtttccgtgg catactcttc cggcccgacc
     3241 accctgaacg cttcatacac ttattcggaa cgctattacg tctcccgtcc cggaaacaaa
     3301 agcgatcaaa gccacgattt tgaatttacc ctgctccaca gcttcaatga gcgttactcg
     3361 gttgacctgc tcgaaagttt tgtggacgcc caggaaccgg aagtcattga cagttccttc
     3421 agcttcccgc ttcgagccaa cggcgataat tatcgcaaca ccgccgcggc taatttgcac
     3481 ggccagatca ccccgttgct tggtttcgtg gttggttatg ccaatagttt ttatgattat
     3541 acgtcagtca cccccggcat tgatggcccc agctacggga cgcttctcaa tcgttttgag
     3601 catttggtga cgctggattc cacttggcgt atttcggaag aaacggtcgg gattttcgga
     3661 tatcaatttc aagcggtgga ttacattgcc cacggctcgt tggctccggg cgcgtttccc
     3721 tatgtttcac cgtcgacgcg cgacagctat aatcacatgg tttatgtcgg cgcccaacat
     3781 agcgtgcgtt ccgatttatc ggtttctttg aaagcgggcg tccaatattc ggattattac
     3841 aacgacacgg ccggcagcaa cccgagttct ctgggcccat ttgttgaact gagcggcgaa
     3901 tacacttaca tggatggagg cacgctcacc gttggattcc gccattcaaa gaactcgacc
     3961 gatgtcggct tttccacagc cacccggacc gttaccgacg accaggaatc atccagcgtt
     4021 tatgcgactg ttcagcaggt gatgactccg ctgtcgccga aactgattgc acgactctcg
     4081 gcgcaatacc aatattcgcg gtttaacggc ggtggtttca acaacgagag cgacgttttt
     4141 tatcttcttg gcctgaacct gacttatcaa ttcacccatt atctttccgc ggaagccgga
     4201 tataactacg atctcctgca atcagatctc cctggccgcg gttatgaccg taatcgggtg
     4261 tacataggcg taaccgcgac ttattgagca acaaacagac agtattacca gggggctggg
     4321 ccgttaagat ctagccccta tttttttgac gtatggatcc tttaaaaact tcttctccgc
     4381 cagaggcaaa acttcatttt ctggattatt ggcggatcat ccgcatccgc aagacggtca
     4441 tccttgccgt cttcctgctg gtcgtcatca cggcgacgat tgtcactttc atcctgccgg
     4501 aagcctattc cagcaaggct gtcatcaagg tcgagaggga tgtcaacgac gtggcgggtg
     4561 ttgctgatca tcccaacatg agcggcgttt acgatcccta tttcatccag actgaatttg
     4621 aagtcattca atccgagaag gtcctggaca aggtcattga caagctcgat ttgaacactg
     4681 catggcagcg gaaatacgga tctgattcca agttcaagac atccgagagc cgtcaagccc
     4741 tcaagcggat gattgaattg cggcctgagc gcaataccat gcttattgac atcactgttt
     4801 acagcgataa caagcaggag gcgatgcaga ttgccaacac ggtcgccgag gtctatagcg
     4861 attaccgcca ggaagaacgc aagaagctta ccttgggcgg catcgaggcg tttaaccagg
     4921 tactcgaggt gcaggatcaa aagatcaagg accaggaggt agttctcgac aagctccgca
     4981 aagatttaaa catcgtcgaa atgggcgacc ccaatgccac gggtgctggc gcttcgatcg
     5041 atcccatcaa cgtccagcat tataacagca tcaaaatcga ggctgaaact tcgtatgtga
     5101 aagaagagaa agtgctgtca aagctcaagg acctttctcc tgaagaactt cgccagacca
     5161 ttcccaccgc cgcgccggac tcgattctcg gcaccttgtt gcaagacctg aactcggccg
     5221 aggaccagct cgtcaccatg cgcagcgatt atggcgatca acatccgcaa gttttgcgcc
     5281 tgatgaagct ccgcgaccgg ttgaatcatg atattgacga gcgggtttcc ggcatcctga
     5341 aagggatgga agccaaagtc gccgccgcca aggctgttgt ccaatatact gaagacaagc
     5401 tctacgaagc caaaaccaac gacatcgcgc gcgcggaaat tggccgtcca tactacatgg
     5461 caaaagttga tttggaacgg ttgcgcaaat tccgcgacgt gctggctctg cgcactttcc
     5521 aggaaactgt tgatattaag atgcccaaaa ccatgatggt ggatctcatt gaacacgcgg
     5581 aagccggtgc gcgacctgtg cgaccgaaca agccgctcaa cattttcttg ggcattgtca
     5641 ttggccttgt ggtgggtgtg ggcttggcct tcttcatcga atatctgga
//
LOCUS       DASZMM010000347         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_131242, whole genome shotgun sequence.
ACCESSION   DASZMM010000347 DASZMM010000000
VERSION     DASZMM010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131242"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VG754_08450"
     CDS             complement(<1..1226)
                     /locus_tag="VG754_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF19284.1"
                     /translation="MKTAKPLPSQAQDYALCISRPLLISILSASLLLPNAFAADWPRW
                     GGNDPGRNMYSPATGLPDHFDVGKFKKGTEEVDLSTTKNVKWVAQLGSQAFGNVVVSG
                     GKVFIGTNNENPRDPQHVGDRSILLCFDEKTGEFLWQLVVPKLASGKVNDWEGLGLLS
                     SPCVEGHRLYVVTSRCEVVCLDTEGMANGNDGPFTDEAQYVVGPGKPKAKIGPKDADI
                     LWRYDMMDELGVFPHNASNCSILIIGDTLYVCTSNGQDWTHSNIPSPNSPSFIALDKK
                     TGKLLGEDDAHIGPHIFHGQWSSPSTGVVNGKQLVFFGGGNGFCYAFDARPVKEGDDN
                     FLKTVWKYDCNPPERWGTDTAHKYPGAEGPSEVNATPVFYKNRVYVAVGQDPEHGEGV
                     GNLTCIDATQTGDVTKT"
     gene            complement(1278..>2855)
                     /locus_tag="VG754_08455"
     CDS             complement(1278..>2855)
                     /locus_tag="VG754_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF19285.1"
                     /translation="AICINVLTGEPIWRIPLTKAGINAAVLVHNNDKVICIYGTPYEP
                     GQLVALKIPHVAPTNAASAPVVVERKDSQVWADDISTSTSSPILANDRVYVVSEKGDL
                     CCVDANSGDLLWKLKIGIEERNSCPLFADGKLYVPILDDPAAKVEGSGESGTKGGFYI
                     IKPTDKSGEIICHVSVDGRCFGTPTAYNGKIYLQTTRHLYCFGKSGNNPGLAAEPAPE
                     KTPTPGAPAQLQIIPSEVLMRPGGTQTFRVRVLDAKGFTVEELKDVSKVKWVSFIPPT
                     AKVKSVMKASFKPDGTLVAANDETPSAGAFEASVDNVKGYMRGRVLPYLPIKQDFEWA
                     KLTETNLMDGNAMFSYPPLPWIGARFKFEIRELEGNKVFAKTIDNQFFQRATVFLGDP
                     STKNYTMEADVMSEGNKRVMSEVGLINQHYCILLKNNDKKLEVNSTPELFRHSVDFQT
                     LPNTWYHLKTRVDVAPDGSGVVRAKVWKKSDPEPAQWTIEVPHLHAHQSGSPGLFSFT
                     LSQKRSYIDNIVVTPNQ"
BASE COUNT          630 a          761 c          802 g          662 t
ORIGIN      
        1 gtcttggtga cgtcgccggt ttgcgtggcg tcaatgcaag tcagattgcc tacgccttcg
       61 ccatgctcgg gatcctgacc aacggcgaca tagacacggt tcttgtagaa cacaggcgtc
      121 gcgttgactt cgctcgggcc ttccgcgccg ggatatttgt gcgcggtgtc ggtgccccag
      181 cgttcaggcg ggttgcaatc atatttccac actgtcttca aaaaattgtc gtcgccttcc
      241 ttgaccggcc gggcatcgaa tgcatagcaa aatccattgc cgccgccgaa gaaaacgagt
      301 tgctttccat tcacaacgcc ggtggagggc gaactccatt ggccgtggaa aatatgcggg
      361 ccgatgtgcg cgtcatcttc gccgagcagt ttgccggtct ttttgtcgag cgcgatgaag
      421 ctgggcgaat tcggcgaggg aatattggaa tgcgtccaat cctggccatt cgacgtgcag
      481 acgtaaagag tgtcgccgat gatgaggatg gagcaattgg aagcgttgtg cgggaaaacg
      541 ccgagttcat ccatcatgtc gtaacgccag aggatgtcgg cgtccttggg accgattttg
      601 gccttgggct tgccgggccc gacgacatat tgtgcttcat cggtgaacgg cccgtcattg
      661 ccgttggcca tgccttcggt gtccaaacaa acaacttcgc aacggctggt gacgacgtag
      721 aggcggtggc cctcgacgca tggcgacgaa agcaaaccaa gcccttccca atcgttgacc
      781 ttgccggaag ccagcttggg aacaaccagt tgccagagaa actcgccggt cttttcgtcg
      841 aagcaaagaa ggatgctgcg atcaccgaca tgctgtgggt cgcgcggatt ttcgttgttc
      901 gtgccgatga aaaccttgcc accggaaaca accacgttgc cgaaagcttg cgaaccaagt
      961 tgcgcgaccc atttgacgtt cttggttgtt gaaaggtcca cttcctctgt gcctttctta
     1021 aatttgccga catcaaaatg gtccggcaat ccggtcgcag gcgaatacat gttgcgaccg
     1081 ggatcattgc cgccccagcg cggccaatca gcggcgaacg cattcggtaa caggaggctg
     1141 gcagacaaaa tgctgataag aagcggacgc gaaatgcaga gcgcgtaatc ttgtgcttgg
     1201 gagggcaaag gcttggctgt tttcatgatt tgcaggatag attttgaaag cggatgcgcc
     1261 ggcgcgatat atgatggtca ttgattcgga gtaacgacga tgttgtcaat atacgatcgt
     1321 ttctgggaca aggtgaagct aaacaaaccg ggcgagccgc tttgatgagc gtgcaggtgc
     1381 ggaacttcta tcgtccattg ggccggttcg ggatcgcttt ttttccagac cttcgcgcgg
     1441 accacgcccg aaccgtccgg tgcgacatcc acgcgagtct taagatgata ccaggtattc
     1501 ggcaacgtct gaaaatccac ggaatgacgg aacaattccg gcgtcgagtt cacctccagt
     1561 ttcttatcgt tgttcttgag caggatgcag taatgctggt tgatgagacc gacttcggac
     1621 atgacgcgct tgttgccctc gctcatcacg tccgcttcca tcgtgtaatt tttggtcgaa
     1681 ggatcgccca ggaacaccgt ggcgcgctgg aagaactgat tatcaatggt cttcgcgaaa
     1741 accttgttcc cctccaattc gcggatttca aacttgaacc gcgcgccaat ccacggcagc
     1801 ggcggataag aaaacatcgc attgccatcc atgagatttg tctccgtaag cttggcccat
     1861 tcaaaatcct gcttgatcgg aagataaggg agaacgcggc cgcgcatgta acccttcaca
     1921 ttgtccacac tggcttcaaa tgcgcctgcc gaaggtgtct catcattggc ggcgaccagt
     1981 gtgccgtcag gcttgaacga ggctttcatg accgacttta ccttcgccgt cggtggaatg
     2041 aacgaaaccc atttaacttt ggaaacatcc ttcaattcct cgaccgtgaa acctttcgca
     2101 tccagcaccc gaacgcggaa cgtttgtgtt ccgcccggac gcatcaaaac ttccgaagga
     2161 atgatctgca attgagcggg tgcgccgggt gtcggcgttt tttcaggcgc aggttccgct
     2221 gcaaggccgg gattgttgcc ggatttgccg aagcaataaa gatggcgcgt tgtttgcaga
     2281 taaatcttgc cattgtaagc cgtcggcgtg ccgaaacagc ggccatccac cgaaacgtgg
     2341 cagatgattt ccccgctctt gtcggtaggc ttgatgatgt aaaagccacc ctttgttccg
     2401 gattcacccg agccttcgac ctttgctgcg ggatcatcaa ggatgggaac gtagagcttg
     2461 ccatcggcga agagcggaca cgaattgcgt tcttcaatcc cgattttcaa cttccacagc
     2521 aagtcgccgc tgttggcatc tacgcaacag agatcgccct tttcgctcac gacatagacg
     2581 cggtcattcg caaggatggg cgaactggtc gaggttgaaa tgtcatccgc ccagacctgc
     2641 gaatctttac gttccacaac gactggcgcg gacgcggcgt tcgtcggcgc aacgtgcggg
     2701 attttcagcg cgacaagctg gcccggttca taaggcgtcc cgtaaatgca aatgaccttg
     2761 tcgttattat gaacgagcac ggcggcgttg atgccggctt tggtgagcgg aatgcgccaa
     2821 atcggttcgc cggtgagaac attgatgcaa atcgc
//
LOCUS       DASZMM010000348        10840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2889706, whole genome shotgun sequence.
ACCESSION   DASZMM010000348 DASZMM010000000
VERSION     DASZMM010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2889706"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VG754_08460"
     CDS             <1..282
                     /locus_tag="VG754_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HWF19286.1"
                     /translation="GWMQIRGTRRRRSLDRGFVLSDHADWTGLLETIDATGAETVWAT
                     HGYTAQLARWLREHGRNAEAIETRFQGEVEEEEAERTGEETPEAPLSDS"
     gene            282..2006
                     /locus_tag="VG754_08465"
     CDS             282..2006
                     /locus_tag="VG754_08465"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037231.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWF19287.1"
                     /translation="MKRFTQLFCELDQSTRTGDKVAALSRYFSEVAPADAAWALHFLS
                     GRTLPRVVGNKQLWQWMTGESNLPEWLIAECYDAVGDMAETIALLLPNAGSGGTQLTL
                     SELVEQRLLPLRNLPDNAKRALLLQTWRELNSNERLVWNKIITGNFRIGAARTLVIRA
                     LAEVANIDPAIMAHRVMGKWQPTPEGFRDLLSNDARATAIAMPYPFFLASPLEMKIER
                     GGPLDELGDIHDWQVEWKWDGIRAQLIRRSGETLIWSRGDEMITDSFPEIVEAGDALP
                     DGTVLDGEILAWRGDSPLPFASLQRRLGRKSVAAKTRESFPVAFVAYDLLEMNGRDLR
                     ATPLTERRRELEKIVSTYFQEIQQRPAVTAKDAHTPLLPGWESHPQLQTSVFNQQTLR
                     VSPLVKADSWTSLAEIQNQSRAKGVEGLMLKKVSSTYGVGRQRGDWWKWKIDPFVIDA
                     VLVAAERGHGRRASLYTDYTFGLWENGQLVPVAKAYSGLTDEEILKVDSFVRTNTTGK
                     FGPVRSVEPLQVFELAFEGIQESTRHKSGVAVRFPRINRWRHDKKPQDADTLEQLLAL
                     AKKGGAES"
     gene            2003..4528
                     /locus_tag="VG754_08470"
     CDS             2003..4528
                     /locus_tag="VG754_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HWF19288.1"
                     /translation="MIEAVPISVNGPVESSHAAALRECLRWFKRHRWKPFDFQREAWA
                     DYLAGKSGLIHAPTGTGKTYGVALPPLIEWLAEFPERKEWPDAVPLRLLWITPLRALA
                     NDTAQSIVKPIADLGLPWTVELRTGDTSAALRAKQRMRFPTVLVTTPESLSLLLSYPG
                     TREKMAGLKSIVVDEWHDLMGTKRGVQTELCLARLRNWFPQLRTWGLSATLGNLEQAL
                     DVLMGNDAPDARLISGDLKKRVEVKTLIPKSVETFPWGGHVGLGLLPQVIKHLEQKRT
                     TLLFTNTRSQTEIWFQALLKARPDWANDIAVHHGSIGREAREEVEQRLRAGTIRCVVC
                     TASLDLGVDFSPVSQVIQVGAPKGVARMLQRAGRSGHQPGAVSRIVCVPAHALELVEF
                     AAVREAIGAGELESRPPMERPLDVLVQHMVTVALGSGFIENDFLAEVRRSHAYRNLTD
                     EEWKWCMDFVIRGGNALRAYPQYSRVVQQGEMFSVPDLFIGRMHRMSVGTITSDSAMA
                     VRVVRGAMLGTIEESFIGRLKPGDKFAFAGFTLELVRIRDMTAFVRKVKKLSGIVPQW
                     MGGRMPLSSQLAVVVRRKLAEARRGTFIGVEMQAVAPVLKIQNLWSHLPEPDELLIER
                     LEMRDGVHHFVYPFAGRLVHEGLAALVAHRVAQLFPASLAVTLNDYGFGLLSGSALSL
                     SDGQWRRVFSWENLLEDLLACLNSAELARRQFREIARIAGLVFQGYPGQGKSTRQLQS
                     SSGLFYDVFARYDSENLLLKQARREVLERQLEINRLKNSLATVQTMKIVTREIPRLTP
                     LAFPLWASFTQANVTSEKWGERVRRMALQLENDMRAENLKNNL"
     gene            4525..5172
                     /gene="pdeM"
                     /locus_tag="VG754_08475"
     CDS             4525..5172
                     /gene="pdeM"
                     /locus_tag="VG754_08475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HWF19289.1"
                     /translation="MIEINCAGEQLRLLPEKAVAWPAARSLIIADLHLGKPAAFRAAG
                     IAVPETTTDADLERLDALLRAHDSRRLIVLGDLLHAPEGMQQAMISTVNQWRANRADL
                     EIILVPGNHDRKCGSPPVSWNMRCVDGEWQCGPFLFSHEPVERAGAYVIAGHVHPALV
                     LREKFGPGVRAACFCFGKSRAILPAFGSFTGMSNLQPAKGDRIFAIGSEEVIEIK"
     gene            complement(5204..8359)
                     /locus_tag="VG754_08480"
     CDS             complement(5204..8359)
                     /locus_tag="VG754_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19290.1"
                     /translation="MKPSSTATLRFDSILSRVSLRSLLLCGLILASATIVQAQVFNYT
                     TLAGYAGQQNADGTNAFITQPVGAAVDGSGNIYVSDFNNNTIRKISSNGTVTTFAGSP
                     GVAGSANGSGTGALFNGPEGVAVDVGGNVYVADAGNDMIRKITPAGLVTTLAGSAGNP
                     GSANGTGTNAFFYAPQGIAVDISSNVYVADYGNHAIRKITPAGVVSTLAGSVGTAGSI
                     NGTGTSALFYEPEGVAVDALGNVYVADTANDMIRVINVAGMVSTLAGSTGNYGSVNAN
                     GTNALFNSPEGVAVDSTGNVYVADTGNNLVRKITVLGAVSTLAGSGNAGSQDGMGTNA
                     QFWGPVGISTDSAGNLYVADYFNGTVRKITSAGAVSTLAGSASNGSADGPDANARFSV
                     AQGVAVDTSSNIYVADAANNTIRKISPNGTVSTFAGLAGSAGSANGAGTNAQFYAPQA
                     VAVDSLGNVYVADTLNHTIREINSAGNVITLAGVAGYSNNADGTGANAQFYAPQGIAV
                     DGSGNVYVADTLNYTIRAITPSGAVSTLAGTAGTYGSADGTNGNALFNWPKGLAVDSA
                     TNIYVADSLNHTIRKVTSAGAVTTLAGSAGVWGSADGTNSNARFFEPDGIAVDSSTNI
                     YVSDSGNHTIRKITPVGTNWVVTTVGGQAANSGSIDGLQSSAFFFYPSGLARDTAGNI
                     YVADSGNNTIRLGTLQPVTTLYNLSVQPRPTTAIITWTTGAPASSQVVYGLTTNYGSS
                     STVNSTLATNHGVLLTGLSASNIYYFKAISQFGPVQGTATGSFSTDPTIIMLSSNASY
                     LGVWVLDSSAQDRYSPSYYYASVAPAAPTASATFQPTIITPAQYDVSLWYSEGGNRSM
                     IAPVTVSFQNGSFSTNMNESVNGGRWQLVTRNQTFAAGASGFVRLGNYTGESNKIVIS
                     DAVKWSYTAGQDIPVNGNVPAWWANFYFGTNNIDSSQSAANGYSILDDYLLGIAPNDP
                     NAHLNFTMRPVAGGVQAIFSPYLGGHSYQLQSTTNFSNPNWQTLANLPVTQDTNGNGV
                     ITASVTNSTSSFYRLSVQLLP"
     gene            complement(8372..10498)
                     /locus_tag="VG754_08485"
     CDS             complement(8372..10498)
                     /locus_tag="VG754_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWF19291.1"
                     /translation="MKYKICGFVCWLYAVTSFAGLPSPSIIPLPEQMQVNAGTFTLCP
                     TTSALGVPAHATTQILVDSNSLATGEYLASLLFRSTGYEFQIATNNGSNAVKGAILLT
                     TVGANTSLGAEGYELTVAPDSVVSRAPAEAGVFYGVQSLLQLLPPQILSVRPVTGVAW
                     VAPCVYIKDQPRFSWRGYMMDESRHFFGKQEVKKFMDAMALQKMNTIHWHLVDDEGWR
                     IQILAYPLLTQTSGWRNGWTNSQNNGVDFGQNPRASTATNGIGQYGGFYTQDEIREIV
                     AYAQQRHITIVPEIEMPAHCTSGLYAYPRYGCGNPQSAYIMDVNSTENINYHVSLFSL
                     GTNTASTNAGVPFLENILTEVAGLFPGPYIHCGGDEVIATGDTQWTNFPTDSNQLAAL
                     GITGTAHNEIIAYQHWFSTNIAAFLKSKGKTMMGWSEFEAGGTVNNAVLEDWETGSSS
                     YAVATAEAGQPVVMAPNAYTYLNYYMSSNNLGSGEYPPFEPYFNSHTYLTLSNVYGFE
                     PVPTNLPAQYTSNILGAECAEWAEYIPSTLNEEFKAFPRLCALAEVEWTQPMQKNYTN
                     FTQRLVAHEQRLTAMGMNYDATNGIVIGTWGPSVPTNTSPPTSAMYDITPYVQSAGDI
                     NLDFHYTSGSKAINVYSASLLVNGAQVDVDTNYIGYSGLSAANLPYFILHLKTFQPGA
                     TYSIQTYYAGYGGTNSNGKVYMVNWN"
BASE COUNT         2392 a         2922 c         3098 g         2428 t
ORIGIN      
        1 ggttggatgc aaattcgcgg gacacgtcgt cgccgctcat tggaccgcgg ttttgtgctc
       61 tcggatcatg cggattggac cggtttgctg gaaacgattg atgccacagg ggcagaaacg
      121 gtttgggcaa cgcacggtta caccgctcaa ttggcccgtt ggctgcgcga gcacggccgg
      181 aatgcggaag cgattgagac aaggttccag ggagaagtgg aggaggaaga ggcggaacga
      241 acaggcgaag aaacgccaga agcgcctctg agcgattcct gatgaaacgg ttcacccaat
      301 tattttgcga actggaccaa agcacccgca cgggagacaa ggtcgcggcg ctgtcgcgct
      361 atttttccga agtcgcgccg gcagacgcgg catgggcatt gcactttctt tccggtcgca
      421 cgctgccccg cgtcgttggc aacaaacagc tctggcaatg gatgaccgga gaatccaact
      481 tgccggagtg gttgatagcg gaatgctacg acgcagttgg cgacatggcc gaaaccattg
      541 cccttctgtt gcccaatgca ggcagtggcg gcacacaact gactctttcc gaactggtgg
      601 aacagcgcct tttgccgttg cgcaatcttc ctgacaatgc aaaacgcgcc ctgcttctcc
      661 agacctggcg cgaactcaat tccaacgagc gcctcgtctg gaacaaaatc atcaccggaa
      721 attttcgcat cggcgctgcg cgcacgctgg tcatccgcgc tcttgcggaa gtcgcgaaca
      781 ttgatcccgc catcatggcg catcgcgtga tgggcaaatg gcagccgaca ccggaaggct
      841 ttcgcgattt gttgtccaat gacgcgcgcg ccaccgccat cgcgatgccc tatccatttt
      901 ttctcgcttc gccgttggaa atgaaaatcg aacggggcgg accgctggac gaactcggag
      961 acatccatga ttggcaggtc gaatggaaat gggacggcat ccgcgcgcaa ctgatccggc
     1021 gcagcggaga aacgctgatc tggtcgcgcg gagatgagat gatcaccgac agcttccctg
     1081 aaatcgtgga ggcgggtgac gcgttgccgg acggcactgt attggacggc gaaattctgg
     1141 cttggcgggg cgactcgccg ctgccattcg cgtcactgca acggcgtttg ggccgcaaga
     1201 gtgtcgctgc aaaaactcgg gaatcgttcc cggtcgcgtt tgtggcttat gacctgcttg
     1261 aaatgaatgg ccgggatttg cgagcaaccc cgctgaccga acgccgccgg gaattggaaa
     1321 aaatcgtttc cacatatttc caggaaatcc aacaacgtcc cgccgtaact gcgaaagatg
     1381 cccacacacc attgttgccg ggatgggaaa gccatcccca gttgcaaacc tctgttttca
     1441 atcaacaaac tctgcgtgtt tcaccgttgg tgaaggcaga ctcatggacc agccttgccg
     1501 aaattcaaaa tcaatcgcgc gccaagggag tggaaggatt gatgctaaaa aaagtaagtt
     1561 ccacttacgg agtaggacgc caacggggcg attggtggaa atggaagatt gatccgttcg
     1621 tgatagatgc tgttttggtg gcggctgagc gcggtcacgg gcgcagggcc agcctgtaca
     1681 ccgattatac cttcggcctt tgggaaaatg gccagcttgt tccggttgcg aaggcatatt
     1741 cgggcctgac agatgaggaa atcttgaaag tggattcatt tgtgcggacg aacacgaccg
     1801 ggaaattcgg cccggtccgt tcggttgagc cgctgcaggt ttttgaactc gcattcgaag
     1861 gcatccagga atcgacgcgg cacaaatcag gcgtcgccgt gcgctttcct cgaatcaacc
     1921 gctggcgtca cgataaaaaa ccgcaagatg ccgatacttt ggaacaattg ctggcgctcg
     1981 caaaaaaggg cggagccgaa tcatgattga agcggtccca atcagcgtga atggtccggt
     2041 cgaatcaagc cacgccgcgg cgcttcgaga atgtctgcgt tggttcaagc ggcatcgctg
     2101 gaagccgttt gatttccagc gcgaggcttg ggccgattat ctagctggca aaagcggttt
     2161 gattcacgcg cccacgggca cggggaaaac atacggggtt gcgcttccgc cgctcatcga
     2221 atggctcgca gaatttcccg aacgcaagga gtggcctgac gctgttccgt tgcgactcct
     2281 ctggatcacg ccactgcgcg cgctggcgaa tgacacagcc caatcaatcg ttaaaccaat
     2341 cgccgatttg ggtttgccgt ggacggtgga actgcggaca ggcgacacat cggccgcttt
     2401 gcgggcaaag caacggatgc gttttccaac ggtgttggtc accacgccag aaagcctttc
     2461 gttattgctc tcgtatcccg gcacgcgcga gaaaatggcc ggattgaaaa gcattgtggt
     2521 ggatgaatgg catgacctca tgggcacgaa gcgaggcgtt caaaccgagc tttgcctggc
     2581 ccgattgcga aattggttcc cgcaactccg cacctggggt ctttcggcaa cgctcgggaa
     2641 ccttgagcag gcgttggatg tattgatggg aaacgacgct ccggatgctc gattgatttc
     2701 cggcgatctg aagaagcgcg tcgaagtgaa gactttgatt ccgaagtctg tggaaacatt
     2761 cccctggggt ggacatgtcg gcttgggttt gctcccgcaa gtcatcaaac atcttgaaca
     2821 aaaacgcacc accttgctgt tcaccaacac ccgttcgcaa acggagatct ggtttcaagc
     2881 gctgctgaaa gcgcggcccg attgggcaaa cgacatcgca gtccaccacg gctccattgg
     2941 ccgcgaagcg cgcgaggaag tcgaacaacg gttgcgcgca ggaacgattc gttgtgttgt
     3001 ttgcacagcg agcctggatt tgggcgtgga tttttcaccg gtttcacagg tcattcaagt
     3061 tggcgcgccg aaaggtgtcg cccgcatgtt gcagcgcgcc ggacgcagcg gacatcaacc
     3121 gggggcagtg agccgcatcg tttgcgtccc ggcgcacgca ttggaacttg tcgaattcgc
     3181 ggcggtgcgc gaggcgatcg gtgcgggtga attggaatcg cgccctccga tggaacgtcc
     3241 gctcgatgtg ctcgtgcagc acatggtgac tgtggcgttg ggaagcggat tcatcgagaa
     3301 cgattttctg gcggaggtcc gccgcagtca cgcataccga aatcttaccg acgaggaatg
     3361 gaaatggtgc atggattttg tgatacgggg agggaatgca ttgcgggctt atcctcagta
     3421 ttcgcgcgtc gttcaacaag gcgaaatgtt ttccgttcca gatttgttca tcggaaggat
     3481 gcaccggatg tcggtgggaa ccatcacgag cgacagtgcc atggcagttc gggtcgtgcg
     3541 gggggccatg ttgggaacga tcgaagaatc gttcatcgga cgcctgaagc cgggcgacaa
     3601 atttgccttc gcaggtttca cgctcgaact ggtgcggatc cgcgacatga cggcatttgt
     3661 ccgaaaggta aaaaagctga gcgggattgt gccgcaatgg atgggcggac gaatgccgtt
     3721 gtcatctcaa ttggcagtcg tcgtccggcg caagctcgcc gaggcgcgtc gcgggacctt
     3781 catcggagtc gaaatgcaag cggtggctcc agtgctgaaa atccaaaatc tttggtcgca
     3841 ccttcctgaa ccggatgaac tgctgattga aagacttgag atgcgggacg gagtgcatca
     3901 ttttgtttat ccattcgccg gacgtttggt gcatgaaggc ctggccgcgc tggtggcgca
     3961 tcgagtggcg caactgttcc cggcttcgtt ggccgtcacg cttaacgatt acggtttcgg
     4021 tttgctttca ggttcagcgc tttcgttgtc ggacgggcaa tggcggcggg ttttttcctg
     4081 ggaaaatttg ctggaagatt tgcttgcctg cttgaattca gccgaacttg ccagacgaca
     4141 attccgggag atcgcccgga ttgccgggct ggtttttcaa ggttatccgg gacagggcaa
     4201 atcgacgcgt caattgcaat cctccagcgg cttgttctat gatgtttttg cgcgttatga
     4261 ctctgaaaac ttgctgctga aacaagcccg ccgcgaagtt ttggaacgac aattggaaat
     4321 caaccgcttg aaaaacagtc ttgcaaccgt ccaaacaatg aaaattgtga cgcgcgaaat
     4381 tccgcggttg acgcctttgg catttccgct ttgggcttcc ttcacccagg cgaatgtgac
     4441 gtccgaaaag tggggtgaac gggtgaggcg aatggccttg caactcgaaa atgacatgcg
     4501 cgcagaaaat ctcaaaaata atttatgatt gagatcaact gtgcgggaga acaactccga
     4561 ctcctgcccg aaaaagccgt cgcatggccc gcggcgcgct ctttgatcat cgcagatttg
     4621 caccttggaa aaccggccgc gtttcgcgcc gcggggattg ccgttcccga gaccacgacc
     4681 gatgccgacc tggaacgcct tgacgcgttg cttcgggccc atgacagccg tcgcctcatc
     4741 gttttgggcg atctgttgca cgctccggaa ggaatgcaac aggccatgat ctcgacggtc
     4801 aatcaatggc gcgcgaaccg cgccgatttg gaaatcatcc tcgttcctgg aaaccatgat
     4861 cgcaaatgcg gctccccgcc cgtgtcatgg aacatgcgct gcgttgatgg cgagtggcaa
     4921 tgcggccctt ttttattttc gcacgaaccg gtggaacgcg ccggcgccta tgtcatcgcg
     4981 ggacatgtcc atcctgccct tgttttgcgc gagaaatttg gacccggcgt ccgcgccgct
     5041 tgtttttgtt tcggaaaatc ccgcgccatt ttgcccgcat tcggaagttt caccgggatg
     5101 agcaacttgc aaccagccaa gggagaccga atttttgcca tcggcagcga agaagtgatc
     5161 gagatcaaat aacctgcccg gaaaaatttt caggcatgtg acatcaaggc aaaagctgca
     5221 cggacaaacg ataaaatgaa gaggtggaat tggtgacact ggcagtgatg acaccgttgc
     5281 cattagtgtc ctgtgtcacc ggcagattgg ccaatgtttg ccagttcgga ttcgagaagt
     5341 tggtcgtgct ttggagttga tagctgtgtc cgcccaagta tggcgaaaaa atggcctgaa
     5401 cacctccagc gaccggccgc attgtgaagt tcagatgcgc gttgggatca tttggagcga
     5461 tgccgagcag ataatcgtcg aggatgctgt aaccattggc agcgctttgg ctggaatcaa
     5521 tgttgttcgt gccaaaataa aaatttgccc accacgcggg aacattgccg ttgacgggaa
     5581 tgtcctggcc agcagtgtaa gaccatttga cggcatcgga gatgacgatt ttgttgcttt
     5641 cacccgtgta attgccgagc cggacgaaac cgctcgcgcc ggctgcgaaa gtttggttgc
     5701 gggtgaccaa ttgccacctg ccgccattga cgctctcgtt catgttcgtc gaaaacgaac
     5761 cgttctgaaa actcaccgtg actggcgcga tcattgatcg attgccgcct tcggaatacc
     5821 agagagaaac atcgtactgc gcgggcgtta tgatagtcgg ctggaatgtc gccgaagctg
     5881 tcggcgcggc gggcgcaacg gaagcataat aatacgaagg gctgtaacgg tcttgcgccg
     5941 atgaatccag gacccacacg ccgagatagg aggcgttcga actgagcatg atgatggttg
     6001 ggtcggtcga aaagctgccg gtggcagtgc cctggaccgg cccaaattgc gagatggctt
     6061 taaaataata aatattgctc gctgatagac cggtcaacaa aaccccatgg ttggtggcca
     6121 aggtggaatt aacagtggaa ctgctgccat aattggttgt cagcccgtaa acaacctgcg
     6181 agctggcggg agcgccggtg gtccatgtga tgatggcggt ggttggtcgc ggctgcacag
     6241 ataaattata aagcgtggtg acaggttgca atgtccccag ccggatggtg ttgttgcctg
     6301 aatcagccac gtaaatattt ccagcggtgt ccctcgcaag tcccgacggg tagaaaaaga
     6361 acgcgctcga ttgcaagccg tcaatgcttc cgctgtttgc agcctgtccg ccgactgtgg
     6421 tgaccaccca atttgttccc acaggcgtga tcttacggat ggtatgattg ccggaatcag
     6481 acacataaat attggtcgag ctgtcaacag cgatgccatc aggttcaaaa aatcgcgcgt
     6541 tgctgttcgt gccatctgcg ctgccccaaa cgcctgccga cccagccaac gtggtcaccg
     6601 cgcccgcgct tgtgactttc cggatggtgt gattaagcga atcagcgacg taaatattgg
     6661 tggcgctgtc caccgccaat cctttcggcc agttgaacaa ggcattgccg tttgtgccgt
     6721 cggcgctgcc gtaagtgccc gccgttcctg ccaatgtgct caccgctccc gagggagtga
     6781 tcgcgcgaat cgtgtagttc aacgtgtcgg cgacatacac atttccggag ccgtccaccg
     6841 cgattccttg cggagcgtaa aactgcgcat tggctccggt gccatccgca ttgttcgaat
     6901 aaccggccac tccggccaat gtgatgacat ttcccgctga attgatttcc cggatggtgt
     6961 ggttcaacgt gtccgcgaca taaacattcc ccaaagaatc caccgccacg gcttgaggcg
     7021 cgtaaaattg agcgttcgtg cctgcgccat tcgcgctgcc agcagagcca gccaatccgg
     7081 cgaaagtgct gactgtgccg tttggactga ttttgcgtat ggtattgttt gcagcgtcag
     7141 ccacgtaaat attgctgctg gtgtccaccg cgacgccttg cgcaacactg aaacgcgcgt
     7201 tcgcatccgg accatcggcg ctgccattgg aagccgaacc cgccagcgtg ctcaccgctc
     7261 cagccgacgt gattttgcgc actgtgccat tgaaataatc cgcaacgtaa agattgcccg
     7321 cgctgtcggt tgaaatgcca accggccccc aaaattgcgc gttggttccc ataccgtcct
     7381 gactgccggc attgccggaa ccggcgagtg tgctcactgc gccgagcacg gtgattttgc
     7441 ggaccaaatt attcccggtg tccgcaacat aaacattccc cgtactgtcc acagccacgc
     7501 cttcgggcga attgaacagc gcgtttgtgc cgttcgcatt gacgctgcca taatttcccg
     7561 ttgacccagc cagcgtgcta accattccag cgacattgat gacgcgaatc atgtcattgg
     7621 ctgtgtccgc gacataaaca ttcccgagcg catccaccgc cacaccctcc ggctcgtaga
     7681 aaagcgcgct ggtgcctgta ccattgatgc tgcccgcagt tcccaccgaa ccagccaacg
     7741 tgcttaccac tcccgccggt gtgatcttgc ggatggcatg gttcccataa tccgccacat
     7801 acacgttcga tgagatgtcc accgcgatgc cttggggcgc gtaaaaaaac gcgttcgttc
     7861 ccgtcccatt ggcgctgccg ggatttccgg ctgaacccgc caacgtcgtg accaatcccg
     7921 cgggtgtgat tttacggatc atgtcgttcc cggcatcggc tacataaaca tttccaccga
     7981 cgtccaccgc cacgccttca gggccgttga agagcgcgcc agttccgctt ccatttgcgc
     8041 tgccggcaac tccgggcgaa ccagcgaatg tggtaacggt tccattggac gaaattttcc
     8101 ggatggtgtt gttgttgaaa tccgaaacgt aaatatttcc cgagccgtcc accgccgcgc
     8161 caacaggttg agtgatgaag gcgtttgttc cgtcggcgtt ctgctgacct gcgtatcctg
     8221 caagagtcgt gtagttgaaa acctgtgcct gcacgatcgt tgctgacgca agaatgagtc
     8281 cgcaaagcag cagggagcgc aatgacacac gggacaaaat ggaatcaaaa cggagggttg
     8341 cggttgagct gggtttcatc gggttgcctc gttaattcca attcaccatg taaaccttgc
     8401 cgttcgaatt cgtgccgccg taaccggcat aatacgtttg gatcgagtaa gtcgcgcccg
     8461 gctggaatgt tttcaaatgc aaaatgaagt agggaagatt ggccgcgctc aaaccggagt
     8521 aaccgatgta atttgtgtca acgtccactt gtgcgccgtt caccaggaga cttgccgaat
     8581 agacgttgat cgccttcgag ccgctggtgt aatggaaatc caggttgatg tctccggcgc
     8641 tttgcacata aggagtgatg tcatacatcg cggaagtggg cggcgatgtg ttggttggca
     8701 ctgaaggtcc ccatgtgccg atgacgatgc cgttggtggc gtcgtaattc atgcccatgg
     8761 ccgtgagacg ctgctcgtgc gcgacaagcc gttgagtgaa gttcgtgtag ttcttctgca
     8821 ttggctgtgt ccactcaact tccgcaaggg cgcacagacg tggaaatgct ttaaattctt
     8881 cgttcaatgt ggacgggatg tactccgccc attctgcgca ttccgcgccc aggatgttgg
     8941 aggtatattg cgccggcaga ttggtcggaa caggttcaaa gccgtaaaca ttgctcaagg
     9001 tcaggtaagt gtggctgtta aaataaggct cgaacggcgg atactccccg gaaccaaggt
     9061 tattgctgga catgtagtaa ttgagatagg tgtaagcgtt gggagccatc accacgggtt
     9121 ggcccgcttc ggcagtcgcc acagcataac tgctgctgcc ggtttcccaa tcttccaaca
     9181 ctgcattgtt caccgtgccg cccgcttcga attcagacca gcccatcatt gtctttccct
     9241 tcgatttgag gaaagccgca atgttcgtcg aaaaccaatg ctgataagcg atgatctcat
     9301 tatgggccgt gccagtgatt cccagcgccg ccaattggtt gctatcagtg gggaaattcg
     9361 tccattgcgt gtcgcctgtc gcaatcactt catcaccgcc gcaatggatg tacggacctg
     9421 gaaaaagccc ggcgacttct gtcaggatat tttccagaaa gggaactccg gcattggttg
     9481 aggccgtgtt cgttcccaag ctgaagagtg agacgtggta attgatattt tccgtgctgt
     9541 tcacgtccat gatgtaagcg ctctgcggat ttccgcaacc ataccgcgga tacgcataca
     9601 gcccggaagt gcaatgcgcc ggcatctcga tttccgggac gatggtgatg tgccgttgtt
     9661 gtgcataagc cacgatttcc cggatttcgt cctgtgtgta aaacccaccg tattgtccga
     9721 tgccgttcgt cgctgtgctg gcgcgtggat tctgcccaaa atcaaccccg ttgttctggc
     9781 tgttcgtcca gccgttgcgc cacccgctgg tttgcgtcaa caatggatag gcgaggatct
     9841 ggatgcgcca gccttcgtca tccaccagat gccagtgaat ggtgttcatc ttttgcaacg
     9901 ccatcgcgtc cataaatttc tttacttcct gctttccaaa aaaatgccgc gactcgtcca
     9961 tcatgtaacc gcgccacgag aaccgcggtt ggtccttgat gtacacacat ggcgcgaccc
    10021 acgccacgcc ggtcaccgga cgcaccgaca aaatttgcgg cggcagcaat tgcaaaaggg
    10081 attgcactcc atagaaaact cctgcttccg ctggcgcgcg cgacacgact gaatccggcg
    10141 cgaccgtcaa ttcgtatcct tcagcgccaa ggctcgtatt tgctcctaca gtcgtcagca
    10201 aaattgctcc cttgaccgcg ttgctgccgt tattggtcgc gatctggaat tcgtatccgg
    10261 tggacctgaa cagcaatgac gcaagatatt cgccggtcgc cagcgaattg ctgtccacga
    10321 ggatctgggt tgtggcgtgt gccggaacgc caagcgcgga agttgtcggg caaagcgtga
    10381 atgtgccggc attgacctgc atctgctcgg gcaaaggaat tatcgaaggt gatggaagac
    10441 cggcaaaact ggtcaccgcg tacaaccaac agacaaagcc acaaatcttg tacttcatta
    10501 caacccgggt ttcgaacgtg aagcgccaga ctagcactcc aatgcaaaaa tgcaagcact
    10561 acgtaagaag atttaatgtc cgagcttgtt cgaaacggaa gattaggaaa agtatgctac
    10621 atcggttcaa gtggccgatc agcggatttc gcgcgactga atgcctaatt ctgagacgtt
    10681 cgcacatcca gactttggac gccgagtcgt gtgatgacgg cccgcataca gtccatatcg
    10741 aacggtttgc cgatcacgta atcaatcgcc agcgagtgcc cgccggtttc aagccgttcc
    10801 ggatacgcgg tgagcaacat cacaggctgg gctggcgcgc
//
LOCUS       DASZMM010000349         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1788809, whole genome shotgun sequence.
ACCESSION   DASZMM010000349 DASZMM010000000
VERSION     DASZMM010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788809"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2238)
                     /locus_tag="VG754_08490"
     CDS             complement(139..2238)
                     /locus_tag="VG754_08490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HWF19292.1"
                     /translation="MAETKSNELMEKIVSLCKRRGFIFQSSEIYGGINGFWDYGPLGA
                     ELKRNVKELWWQNMIRFRDDVVGLDATIIMAPEIWRASGHVETFADLMRECPLTKKRF
                     RADQVEPQSGIAYHYQSVVEDSYYYYLHSKEFSDAIPGWQEAVAQSTGVSRSPELETL
                     VVKHLVASSETAKRQMGKQFLEASRSSWSQNLPYSVLLAPGKPPESARKLATQFYQQR
                     GYIHPKLLGERIEKVENSTQYNPENGGLLSDPRPFNLMFKTYIGPVSVPPIPHFDYGD
                     EGVAVDELKQWAEAFNECEKNVAYLRPETAQAIFAQFKNVLETSRQSLPFGIAQIGKA
                     FRNEVTPRNFTFRSREFEQMELEFFIKPDEAVEAICGRVANAEEALGVPASAGSSEPP
                     KGGTPSPEWGWQTWHKYWVEERIRFYEGIGLPRQSLVEYWQKPDELAHYARATVDILY
                     KFPFSKRDEKGELAGEELEGVAARSDYDLSQHERFSGKPMSVFDEDLRAAWGKLDDAK
                     KKELSDRYYQARLKYLTKAGVPAEKAEKEAKEDAEGLAKGQYIPHVIEPSAGVDRLVL
                     ALICNAYNEEQAPDEKGKMETRVVMKFHPRIAPIKVGVFPLLKNKPDLVKKAQQVRDL
                     LRPHMSVFYDEAGAIGRRYRRQDEAGTPFGVTIDFDTLGEKPELLDTVTLRERDTMQQ
                     TRVRIDALLPMLMEKIR"
     gene            2639..>2928
                     /locus_tag="VG754_08495"
     CDS             2639..>2928
                     /locus_tag="VG754_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HWF19293.1"
                     /translation="MSNARFELVRVPSLKTVENFRRHLASLNLTLPCDEKVETGGRSP
                     LTRPVENVVINGKTIGNRIAVQPMEGWDGTTDGGITEEVLRRWTRFGQSGAKL"
BASE COUNT          634 a          809 c          771 g          714 t
ORIGIN      
        1 attgttgtcg gaaagcaaat aaagccccag cgcgaggctg gcgaacagaa gacctttcac
       61 aagcttgtac gcgatgatgg cgtaaagcgt cggcgcacgc ttcaacaccg ccttcggttt
      121 gtttgcctcc tcggccatct atcggatttt ctccatgagc atcggcagca aggcgtcgat
      181 cctcacccgt gtctgctgca tcgtgtcgcg ctcgcgcaag gtgacggtgt ccagcaactc
      241 gggtttttcg cccagcgtgt cgaaatcaat cgtcacgccg aacggcgtgc ccgcctcgtc
      301 ctggcggcga taacgccggc caatcgcgcc cgcctcgtca tagaataccg acatgtgcgg
      361 acgcaagagg tcgcgcactt gctgcgcttt tttgaccaga tcgggcttgt tcttgagcaa
      421 cgggaacacg cccaccttga tcggcgcgat gcgcggatgg aatttcatca ccacgcgcgt
      481 ttccatcttt cctttttcgt ctggcgcctg ttcttcgtta taggcattgc aaatcaatgc
      541 gagcaccaga cggtccacgc ctgcggatgg ttcgatgacg tgcgggatgt attggccctt
      601 cgccaaacct tcggcatctt ccttggcttc cttttccgct ttctcggcag gcacaccggc
      661 cttggtgaga tatttcaggc gggcttgata atagcgatcc gataattctt ttttcttcgc
      721 gtcatccagc ttgccccaag cagcgcgcaa atcttcgtca aacacgctca tcggcttgcc
      781 ggaaaaacgc tcgtgctggc tcaagtcgta atcgctgcgg gcggcgacgc cttccagttc
      841 ttcaccggcg agttcgcctt tttcgtcgcg cttgctgaac ggaaatttgt aaaggatgtc
      901 caccgtcgcg cgcgcgtaat gggcgagttc atccggcttc tgccaatatt caaccagcga
      961 ttggcgcggc agaccgatgc cttcatagaa ccgaatgcgt tcctcgaccc aatatttatg
     1021 ccacgtttgc cagccccact ccgggctggg tgtcccgcct ttaggcggct cggatgaacc
     1081 ggctgaagcc gggacaccaa gcgcttcctc ggcgttcgcg acacggccgc aaattgcttc
     1141 gaccgcttca tcgggcttga tgaaaaattc caattccatc tgctcaaatt cgcgcgaccg
     1201 aaaggtgaag ttgcgtggcg ttacctcatt gcgaaacgcc ttgccgatct gcgcaatgcc
     1261 aaacgggagg gattgccgcg aagtttcgag cacatttttg aactgcgcaa agatggcttg
     1321 agcggtttca gggcggaggt aagcgacatt cttttcgcat tcattgaatg cttctgccca
     1381 ctgtttcaat tcgtcgaccg cgactccttc atcgccataa tcaaaatgag gaatgggagg
     1441 aacgctaaca ggtccgatat acgttttgaa cattaggtta aacggacgcg gatcgctcaa
     1501 aagaccacca ttttcaggat tatattgtgt tgaattctca actttttcga tacgctcccc
     1561 aagcaacttt ggatgaatgt acccgcgttg ttgataaaac tgtgttgcaa gcttccgcgc
     1621 ggattcagga ggcttgcctg gagccaaaag aacagagtat ggaagatttt ggctccagga
     1681 cgatcttgac gcctccagaa actgttttcc catttgccgc tttgctgttt cagagctggc
     1741 gacaaggtgc tttaccacta aagtctccag ttctggactg cgagaaacac cagtgctctg
     1801 agccactgct tcttgccagc ctggaattgc atcgctgaat tcttttgaat gaagataata
     1861 ataataagag tcctcaacga cactttgata gtggtatgca attccggatt ggggttcgac
     1921 ttgatccgct cgaaatcttt ttttcgtcaa tggacactcc cgcatcaaat ccgcaaacgt
     1981 ctccacatgc cctgacgcgc gccaaatttc cggggccatg atgatggtcg catccaggcc
     2041 gacgacgtca tcacggaagc ggatcatgtt ttgccaccaa agctccttga cgttgcgctt
     2101 caattccgcg ccaagcgggc cgtaatccca aaagccgttg atgccgccgt agatttccga
     2161 ggattgaaaa atgaagcccc ggcgcttgca cagcgacacg attttttcca tcaattcatt
     2221 gctcttcgtt tcggccatag gaagggtcag ttttctccat gcaacggcgt gtgtcaagat
     2281 ggctgacagg ggaaacaacc gcagatggtg catcttgcca ctgttcttaa aagaatgtac
     2341 gggtacggtt tggatgaagc tcgccctcac ccaaaccctc tcccccaggg agagggcttt
     2401 cacagtttcc cgctgttcgg aacattcccg cgatggaatg gtgcaaacga ttgcggccaa
     2461 tgataaaacg gggcgggcgc cggttctcct tctccaaggg ggagaaggcc ggaatgaggg
     2521 cggaccttct ctcgtttttc acaatgcatt caagaacggt atcaggatgc gccccgagcg
     2581 tccgagcccg gtgataattt gcctgacagt cacggaaatt acggtcagtt tcttgggcat
     2641 gtctaacgcg cgatttgaat tggtacgcgt cccgtcgctc aagacggtcg agaattttcg
     2701 caggcatctc gcttcgttga acctcacttt gccctgcgat gaaaaagtgg agactggtgg
     2761 gcgttcgcct ttgactcggc cggttgagaa tgttgtcatc aatggcaaaa ccattggcaa
     2821 tcgcatcgcg gtccaaccaa tggaaggttg ggacggaacg accgacggtg gcatcaccga
     2881 agaggtgttg cggcgctgga cgcggttcgg ccaaagcggc gcaaaact
//
LOCUS       DASZMM010000350         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4277966, whole genome shotgun sequence.
ACCESSION   DASZMM010000350 DASZMM010000000
VERSION     DASZMM010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4277966"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..1944)
                     /locus_tag="VG754_08500"
     CDS             complement(475..1944)
                     /locus_tag="VG754_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19294.1"
                     /translation="MKPILCIPSSLCLAASCFSAFLLLAIPGCATKSSTVGANVGATE
                     DKQLQAGMAEVDITPPIGFRMAGYFYERFSTATHDPLMAKALVLRQGREEVAMVFCDL
                     VGLSLHVTAKARALASQQTGIPVSHIVIAATHSHTGPLFDDVRGDFFHQTALAKTGDD
                     SHQTIDYPAFLTGQLVKAIVLARSKLGPAELDAGIAPQPGLAFNRRYYMKDGTVRFNP
                     GILNSNIVAPAGPTDPTVSMLIVKSRERQQAIGGLTVFAMHCDTIGGTKFSADYPCFI
                     QQTLRKNFGPSYVSAFGAGACGDINNIDVSKKQNYSGFDVSERLGDAIGNTVVQNFPN
                     LKHIAAPSLAVKSATLMLPLQTVTPFEVATAKIAMGHLDDPKTDFTMRVKTVKILDLT
                     RRGAILPMEVQVFRLDADTAIVCLPAENFVEFGLAIKKASPFKTTFVITICNDRPAYV
                     PTLKAFKEGSYEVLNSRVKPGGGEAMMDAAIGLLNELKQ"
     gene            complement(2833..>3639)
                     /locus_tag="VG754_08505"
     CDS             complement(2833..>3639)
                     /locus_tag="VG754_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HWF19295.1"
                     /translation="EAGAKITLFHDELNPLFGGHHPEPNAENMSDVSKFVRSGKAQLG
                     LGTDGDADRFGIVDKDGTWLTPNQVLALALYHLKKNRHWTGAVVRTVPTSHQVDAVAK
                     LFGVKVYETPVGFKYIGALMESEPIIVGGEESGGLSVKGHVPEKDGILACLLMAELVA
                     TEKKSLGQILKALEKQTGEFHTDRINVAIPPEKKEALLAKLAAGLSSVGPFKVEKFIT
                     TDGYKFLLPEGEWVAFRASGTEPLIRCYIEAKSAAHLKKLRQACQDLLKV"
BASE COUNT          838 a          963 c          950 g          888 t
ORIGIN      
        1 aatcatgatc cttgtcatca cgcttgagat aagccactaa cggtccggca tcggcaatta
       61 ccgtttccat gagccataac cggctaaatg cttctttttc cttgcaagat cacgagggcc
      121 gcccgttgtt gcgccacaaa ggtcccggct gagttcataa agcgatgaat ggcgtgaatc
      181 agtgtgcttg gtcgagttca agcgtttctc caaagtctcc cgtatgaatc gcgcggggga
      241 tttgccggaa cgccgggcaa taatggctag tcggcgcttc aaattgtcgg gcagttggag
      301 cgttagtgtg ctcatgcgag caatctagcc agtctctgtg cgagtggcaa gaatgagatt
      361 ttaactgatg gaacaacgat tctgacagaa ttaacagaat gaaacagaat cgaacaaatc
      421 cgaattttca ttctgatcat tctgtttatt ctgtcaaaat tttattgtgt agttctattg
      481 cttcaactca ttcaacaacc cgatggcagc gtccatcatc gcttcgccgc cgccgggttt
      541 gacgcgtgag ttcaagactt cgtaacttcc ctccttgaac gctttcaacg tcggcacgta
      601 agccggacga tcattgcaga tggtgatgac aaaggtggtt ttgaatggcg acgctttttt
      661 gatcgccagt ccgaactcga caaaattttc agcgggcaga cagacgattg ccgtatcggc
      721 atccaaacga aagacctgga cctccatcgg caagatagcg ccgcgtcgcg taaggtccaa
      781 tatcttcact gttttgaccc gcatggtgaa gtcagttttg gggtcgtcca aatggcccat
      841 ggcgattttg gcggtcgcaa cttcaaacgg agtcacagtt tgcaatggaa gcatcagagt
      901 ggcgcttttc acggcgagtg atggggcggc gatgtgtttg agatttggga aattctggac
      961 gactgtattg ccaatagcat cacccagccg ttccgagaca tcgaacccgg agtaattctg
     1021 ttttttggaa acatcaatat tattaatatc gccgcaagcg cccgcgccaa aggcggagac
     1081 atacgacggc ccgaaattct tccgcaaggt ctgctggatg aagcaaggat aatcggcgct
     1141 gaatttcgtg ccgccaatgg tatcacaatg catcgcaaaa accgtcagcc cgccgatggc
     1201 ctgttgacgc tcgcggcttt tcacgatgag catgctgacg gtggggtcgg ttggaccggc
     1261 gggcgcaacg atgttggaat tcaaaatgcc cgggttgaac ctgacagttc catctttcat
     1321 gtaataacgg cggttgaaag caagccccgg ttgcggcgcg atgccagcgt ccaattcagc
     1381 aggtccaagt ttcgaccggg caagcacgat ggctttgacg agttgtccgg tgaggaacgc
     1441 gggataatca atggtttgat gcgagtcgtc gccggttttg gcgagggcgg tttggtggaa
     1501 aaagtctccc cgcacatcat cgaacaaggg cccggtatga ctgtgcgtcg cggcgatgac
     1561 gatatgcgag acgggaatgc cggtttgctg gctggccaat gcgcgcgcct tggcggtcac
     1621 gtggagcgaa agaccgacca aatcgcaaaa aaccatggca acctcttccc tgccctgccg
     1681 caaaaccaat gccttcgcca tgagcggatc atgagttgcc gtggaaaacc gttcgtaaaa
     1741 atagcccgcc atgcggaacc caatcggcgg cgtgatgtcc acttccgcca ttcctgcctg
     1801 caattgttta tcctccgtcg cgcctacatt ggcaccaact gtgctgcttt ttgtggcaca
     1861 gccgggaatg gcaagcagca ggaacgcaga aaagcaggat gccgcgaggc acagactgga
     1921 tgggatgcag agaatgggtt tcatcaactg ctttcacgat gaataagcct ttgcgctgct
     1981 tcaagcataa ttaaatcttg agcaacactt tgagcatggt gtccaagaat tgaacctaac
     2041 aaatggattt caagctgtta tttagatccg tcgtggacgc ctgccagaat tcccctcttc
     2101 tggtcgggct ggctgtattg gccttcgccg tttcgatttt cctcatcatt gacgcgcatc
     2161 ggaacaagaa aaagcgcgcc cggcaccgtt ggaagtaagg aaaagggcca aaatcgccca
     2221 aaattcgggt ttcaccccat ttcgagccag aataatctgg gtaattttca cttacgtaca
     2281 caacatctcc ccgctgacca aggaggggcg gggttctttt ttttcaaaaa actcccaaga
     2341 atcttcattt gaagggaaat ttgcgttttg gcgttctgaa accttgaccg ttgaaagggg
     2401 acggtccccg gatagggccg ccccccaact aacatccctc aatgaggaac cacaaataac
     2461 atcgcaggaa ttgcacattt tgcaatgggt gcgggctaaa atttgggcct gaagaattgt
     2521 taagaatgcg tgtcaactgc tgttcgagct tgccaacctt gtgagcgttt tgtaatgtct
     2581 cccgcgtagt ggccacttcg gccgtcgtaa gcagtccgca aaaaccagat cgcttttgcc
     2641 atgagcttgt caaaaatgat acgcgaattg tttgtgcccg gatctcgcgc ttttggcgat
     2701 atgcgccgcc gtcagcgccg gattgtgatt ctgtccctca tgttcgcgat gataattgct
     2761 gtcgcgtttg ggctcgcgct ttatcggttg aacgcatcgg gacgattcca gcattaaacg
     2821 ctttgcattc gctcaaacct tcagcaaatc ctggcacgcc tgccgcagct tcttcaggtg
     2881 cgcggctgat ttcgcctcga tgtagcaacg gatgagcggt tccgtgccgc tggcgcggaa
     2941 cgcaacccat tcgccctcgg gcaaaagaaa tttatatccg tctgtagtga tgaatttttc
     3001 caccttaaac ggcccgacag acgaaagccc agccgccaat ttcgccagca gcgcctcttt
     3061 tttctccggc ggaatcgcca cgttgatgcg gtcggtgtgg aattcgcccg tttgtttttc
     3121 cagagctttc aaaatctgac ccaaggattt tttttctgtc gcaacgagtt cagccatcaa
     3181 gagacacgca agaatgccgt ccttttccgg cacatggcct ttgacgctca aaccgccgga
     3241 ttcctcaccg cccacgatga tcggctcgct ttccatcagc gcgccgatgt atttgaaccc
     3301 gaccggcgtt tcataaactt tcaccccaaa caatttcgcc accgcatcca cttgatggct
     3361 cgtgggcacg gtgcgcacca ccgcgccagt ccaatgccgg tttttcttca agtgatacaa
     3421 cgccaatgcg agcacctggt tgggcgtaag ccaggtgccg tccttgtcaa cgatgccgaa
     3481 acggtcggca tcaccatccg tgccgaggcc gagttgcgcc tttccgctgc ggacgaactt
     3541 gctgacgtcg ctcatgtttt cggcgttcgg ttcgggatga tgaccgccga acagcggatt
     3601 caattcatca tgaaacaagg tgattttcgc gccggcttc
//
LOCUS       DASZMM010000351         5159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5362694, whole genome shotgun sequence.
ACCESSION   DASZMM010000351 DASZMM010000000
VERSION     DASZMM010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5362694"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /gene="fusA"
                     /locus_tag="VG754_08510"
     CDS             <1..1446
                     /gene="fusA"
                     /locus_tag="VG754_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWF19296.1"
                     /translation="AVSNKDDAIAELVLEEKPIDPLVLKAAIRRLTCKIELVPVLCGS
                     AFKKKGVQVLVDAVIDYLPSPLDVPVAEGHEPGTTDVVKVDTNDNSKFCSLAFKLWTD
                     PYAGKLVFFRVYSGQLKKGDTIYNPRTRKRERVSRLMIIQGSERKDIDHVYAGDIAAL
                     VGLRNITTGDTLCDEDFDVMLEPPTFPEPVISMAVEPKTKADRDKMSEGLQRLAEEDP
                     TFRCFTNEETSQLIIAGMGELHLEIIIDRLKREFKVDANTGAPQIAYRETITKPADGE
                     GKFIRQSGGRGQYGHALVKVEPNEKGKGVEINNKIVGGAIPREYIPAVVDGIEEAVRG
                     GVYAGYQVIDIKVDIVDGTFHEVDSNELAFKMAGIFALKDAFKKAGPILLEPIMKVEV
                     TTPDEYQGDLLGDINRRRGTIIGIEAKSGQTILNANVPLAEMFGYATAIRSLSKGRAS
                     YSMEPLTFEQVPNSVLTTILDSSKSRPAART"
     gene            1475..1783
                     /gene="rpsJ"
                     /locus_tag="VG754_08515"
     CDS             1475..1783
                     /gene="rpsJ"
                     /locus_tag="VG754_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWF19297.1"
                     /translation="MAGQRIRIRLKAFDHRVIDQSARDIADTVKRTGARVAGPIPLPT
                     RVERFTVLRSPHSDKKSQETFEQRTHKRLLDIIEPTAKTVDELKKLNLPAGVDITIKI
                     "
     gene            1788..2447
                     /gene="rplC"
                     /locus_tag="VG754_08520"
     CDS             1788..2447
                     /gene="rplC"
                     /locus_tag="VG754_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWF19298.1"
                     /translation="MLGLLGKKLGQTRVYDEKGVLIPVTVVLAGPNRVLQCKTQESDG
                     YNAVQLGFDDQKDQRVTKPLLGHIKKHNGAAVKRIREFRNFSVSVKPGDVVGANIFAK
                     GDFIDAIGVTKGRGFEGVMKRHNFRGGDASHGAKGWRRRPGAIGQRLFPGTVMRGMRM
                     PGHMGQVQRTVQNLEIIQVREADNLLLIKGAVPGSKGDYVVIREAKKNPKAKVAEAKK
                     K"
     gene            2457..3080
                     /gene="rplD"
                     /locus_tag="VG754_08525"
     CDS             2457..3080
                     /gene="rplD"
                     /locus_tag="VG754_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWF19299.1"
                     /translation="MKLKIQDIKGNSHGELEVKFALIENGKGTQAVHDTVVAYNAAQR
                     SGTACTKTAGEVAGTNKKPWKQKGTGRARAGSFQSPLWRGGGVVFGPRPRDFSKKVSR
                     STRQLALRKALSERLHAGDVVVVDDIKLDSNKTKEFIGILSTLKLDGSTLIVSHATDK
                     NLSLASRNVPNVKLTTSDELNTYEILRSDKLVFTRGAFEKVEERLSK"
     gene            3089..3370
                     /gene="rplW"
                     /locus_tag="VG754_08530"
     CDS             3089..3370
                     /gene="rplW"
                     /locus_tag="VG754_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWF19300.1"
                     /translation="MNSFEIVKTVRLTEKGTRQGEKLNQYTVVADRRANKVQIRQAVQ
                     ELFKVKVVRVNTMNVHGKDRRQRTTQAGKTSDWKKAIVTLKEGDKITLT"
     gene            3384..4229
                     /gene="rplB"
                     /locus_tag="VG754_08535"
     CDS             3384..4229
                     /gene="rplB"
                     /locus_tag="VG754_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWF19301.1"
                     /translation="MPQKVYRPLTPSTRYITIASYADITKRRPEKSLVFTRKKTGGRN
                     SYGRITSRGIGRGNKQKIRNVDFRRDKHGVEATVSSIEYDPRRTARLALLQYSDNEKR
                     YIICPVGLQVGTKLMSGPTAAPETGNNLPLRSIPVGVPIHNLELIPGKGGQMVRSAGG
                     AATLMSRDEGYAQVRLPSGEIRKIHVDCYATIGQVGNTDHENVVLGKAGRSRHRGIRP
                     LSRAVAKNPVDHPMGGGAGKTSGGGHPVTPWGVITKGKKTRNRRKLSNRFILVRRDGR
                     PMKLK"
     gene            4236..4517
                     /gene="rpsS"
                     /locus_tag="VG754_08540"
     CDS             4236..4517
                     /gene="rpsS"
                     /locus_tag="VG754_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWF19302.1"
                     /translation="MGRSIKKGPFVEGHLLEKITKLNAVGSKKPIKTWSRRSMITPEF
                     VGHTFNVHNGKVFNPVFVTENMVGHRLGEFSLTRTFKKHGAHTAKAEAK"
     gene            4522..4878
                     /locus_tag="VG754_08545"
     CDS             4522..4878
                     /locus_tag="VG754_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HWF19303.1"
                     /translation="MQVQAITRNVNMSAQKMREVVRQIQGLPALHAQAVLAVVPRKSA
                     RFVAKTLKSAIANAEDQARHQKNNQFKTENLRVHSAVAGTASTANRKRFTPKARGSAG
                     PIIKRTSHIKIVLSDE"
     gene            4884..>5159
                     /locus_tag="VG754_08550"
     CDS             4884..>5159
                     /locus_tag="VG754_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWF19304.1"
                     /translation="MGQKTNPIGLRIAVNKDWRSKWYAGKKEFGKLLSEDRDIRDLLK
                     KKLESASVPKILIERAATRCRITILTARPGVVIGRKGAEIDKLKEELS"
BASE COUNT         1263 a         1463 c         1452 g          981 t
ORIGIN      
        1 gccgtttcca acaaggacga cgcgattgcc gaactcgttt tggaagaaaa gccgattgac
       61 ccgcttgttc tcaaagccgc cattcgccgt ttgacctgca agattgaact ggttccggtc
      121 ctgtgcggtt cggcctttaa gaaaaaaggc gtgcaagttt tggtggatgc cgtgattgat
      181 tatcttccca gcccgctgga tgttccggtt gccgaaggtc atgaaccggg gacgaccgat
      241 gtcgtcaaag ttgataccaa cgataacagc aaattctgct cgctcgcttt caagctgtgg
      301 accgatcctt acgccggcaa gctggtgttc ttccgcgttt attcgggcca gttgaagaag
      361 ggcgatacca tttacaatcc gcgcacgcgc aagcgtgaac gcgtcagccg cctcatgatc
      421 attcaaggca gcgaacgcaa ggacattgat cacgtttacg cgggcgacat cgccgcgttg
      481 gtcggtttgc gcaacatcac gaccggcgac acactttgcg acgaggattt cgacgtgatg
      541 cttgaaccgc cgacgttccc cgaaccggtc atcagcatgg ctgtcgagcc gaagaccaag
      601 gccgaccgcg acaagatgtc cgaaggtttg cagcgtctcg ccgaggaaga cccgacgttc
      661 cgctgtttca cgaacgagga aaccagccag ctcatcatcg cgggcatggg cgagttgcat
      721 ttggaaatca tcatcgaccg cctcaaacgc gagttcaagg tggacgccaa caccggcgcg
      781 ccgcagatcg cgtatcgcga aaccatcacc aaaccggctg atggcgaagg caaattcatc
      841 cgtcaatccg gcggtcgcgg tcaatacggc catgcgctcg tcaaagtcga gccgaatgaa
      901 aaaggcaagg gcgttgagat caacaacaag attgtcggcg gcgcgattcc gagggaatac
      961 atcccggcgg tcgttgacgg catcgaagaa gccgttcgcg gcggtgtcta tgccggttac
     1021 caggtgattg acatcaaggt cgatatcgtg gacggcacgt tccacgaagt tgactcgaac
     1081 gaactcgcgt tcaagatggc cggtattttc gcgctcaagg atgccttcaa aaaggccggc
     1141 ccgattttgc tcgagccgat catgaaggtg gaagtcacca cgcctgatga atatcagggc
     1201 gatctgctcg gcgacatcaa tcgccgccgc ggcaccatca tcggcattga agccaagagt
     1261 gggcagacaa tcctgaacgc caatgtgccg ctggccgaaa tgttcggtta cgccacggca
     1321 atccgttcgc tttccaaagg ccgcgcttct tactcgatgg aaccgctcac gttcgagcag
     1381 gttcccaaca gtgttttaac cacaattctc gacagttcca aatcaagacc ggctgcgcgt
     1441 acataacaac caaaactata caattgctct ttctatggct ggacaacgca tacgcattcg
     1501 actcaaagct tttgaccatc gggtcatcga tcaatccgct cgtgacatcg ctgataccgt
     1561 caaacgcacc ggcgctcgtg tcgccggacc catcccgctt ccaacccgcg tggaacgctt
     1621 caccgtcctg cgctcgccgc actcggacaa gaagtcgcag gaaaccttcg aacaacgcac
     1681 ccacaagcgc ctgcttgaca tcatcgagcc gaccgccaag accgtggatg aattgaagaa
     1741 actgaacctg cctgcgggcg tggacatcac catcaagatt tgattttatg ctcggcttac
     1801 taggaaaaaa attgggacag acgcgcgtct atgacgaaaa aggcgtcttg atccctgtca
     1861 ccgtggtgct cgcgggaccc aaccgggtgt tgcaatgcaa gacccaggaa tccgacggct
     1921 acaacgcggt gcaactcggt ttcgacgacc agaaagacca acgcgtcacc aagccgttgc
     1981 tcggccatat caagaaacac aatggcgcgg cggtgaaacg catccgtgag ttccgtaatt
     2041 tctcagtttc ggtcaagccc ggcgatgtgg tcggcgcgaa catttttgcg aagggcgatt
     2101 tcattgatgc catcggcgtc accaagggcc gtggttttga aggtgtcatg aagcggcaca
     2161 actttcgcgg tggtgatgct tcgcacggag ccaagggctg gcgtcgtcgt ccaggcgcga
     2221 ttggccagcg tttgttcccc ggcacagtca tgcgcgggat gcggatgccc ggtcacatgg
     2281 gccaggtgca acgcaccgtc cagaatttgg aaatcatcca ggtgcgcgag gcggacaacc
     2341 tccttttgat caagggagcg gtgcccggtt ccaagggcga ttacgtcgtc attcgtgagg
     2401 ccaagaaaaa tcccaaggcc aaagttgccg aggccaagaa aaaataacgg actcacatga
     2461 aacttaagat tcaagacatc aaaggcaaca gccatggcga actggaagtg aagttcgcgc
     2521 tcatcgagaa cggcaaaggc acgcaggctg tccacgatac cgttgtcgct tacaacgccg
     2581 cgcagcgcag cggcacggct tgcaccaaga ccgctggcga agtcgccggc acgaacaaaa
     2641 agccctggaa gcaaaagggc acaggccgcg ctcgcgccgg ttctttccaa tcgcctttgt
     2701 ggcgcggtgg tggtgtggtt ttcgggccga ggccccgcga tttcagcaag aaagtttcgc
     2761 gttcgacgcg ccagctcgcg ttgcgcaagg cgctcagcga acgtttgcat gccggcgatg
     2821 tggtcgttgt tgatgacatc aagctggact cgaacaagac gaaggaattt atcggcatcc
     2881 tttccacgtt gaaactggat ggctcgactt tgattgtttc gcacgcgacg gacaagaatt
     2941 tgtctctggc ctcgcggaac gtcccgaacg tcaagctgac gaccagcgac gaattgaaca
     3001 cgtatgaaat tttgcgcagc gacaagctgg tgttcacccg cggggcattt gaaaaagttg
     3061 aagagcgtct cagcaaataa atttcaccat gaactccttt gaaatagtca aaacggtgcg
     3121 tctcacggaa aaagggacgc ggcagggtga aaagctgaac caatacaccg tggtggccga
     3181 tcgccgcgcg aacaaggtac agatccgcca ggcggtgcag gaattgttca aggtgaaggt
     3241 cgtccgcgtg aacaccatga atgtgcatgg caaggaccgc cgccagcgca cgacccaggc
     3301 cggcaaaacc tccgactgga agaaggcgat tgtgaccctc aaagagggcg acaaaattac
     3361 tttgacctaa actgatttaa gttatgccgc aaaaagttta cagaccgctg acgccgtcca
     3421 cgcgatacat caccatcgcg tcgtacgccg acatcaccaa gagacgcccc gaaaagagcc
     3481 ttgtgttcac ccgcaagaaa acgggcggac gcaattccta tgggcgcatc accagccgcg
     3541 gcatcgggcg gggcaacaag caaaaaatcc gcaacgtgga ttttcgccgc gacaagcatg
     3601 gggttgaagc aaccgtctcc tcaatcgaat acgatcctcg ccggacagcg cgtttggcgc
     3661 ttttgcaata ctccgacaac gagaagcgat acatcatttg ccctgttggc ttgcaggttg
     3721 gtacgaaatt gatgagcggg ccgacagccg cgcccgaaac cggcaacaat ttgccgttgc
     3781 gctcgattcc agttggcgtg ccgatccaca atcttgaact gattcccggc aagggcggtc
     3841 agatggttcg ttccgcgggt ggagcagcca cgttgatgtc acgtgacgaa ggttatgcgc
     3901 aagtccgttt gccttccggc gaaattcgca agattcatgt ggattgttat gccaccatcg
     3961 gccaggtggg caacactgac catgaaaatg tcgttttggg caaggctgga cgcagccgtc
     4021 atcgcggcat ccgtccgttg agccgcgcag tcgcgaagaa cccggtggac catccgatgg
     4081 gcggcggcgc aggcaagacc tccggcggcg gacatccggt gacgccttgg ggcgtcatca
     4141 cgaagggcaa gaaaacccgc aaccgccgca aattgtccaa ccgctttatc ctggtgcggc
     4201 gcgatggccg gccgatgaaa ctgaaataaa aatttatggg acgttcgatt aaaaagggcc
     4261 cgtttgtcga gggccatctg ctggaaaaaa tcacgaagct caacgctgtc ggttccaaga
     4321 aaccgatcaa gacgtggtcg cgccggtcca tgatcacgcc ggaattcgtc gggcacacgt
     4381 tcaacgtgca caacggcaaa gtattcaacc cggtgttcgt gaccgaaaac atggtcggac
     4441 atcgcctcgg cgaattttcg ctgacccgca cgttcaagaa acacggcgcg cacacggcca
     4501 aggccgaagc caaatagttt tatgcaagtt caagccatca cccgcaacgt caacatgtcc
     4561 gcgcagaaga tgcgcgaagt ggtccgccag atccagggcc tgccggcgct gcacgcgcag
     4621 gctgtcctcg cggtggtgcc gcgcaagtcg gcccgtttcg tcgcaaagac cttgaaatca
     4681 gccatcgcca acgcagaaga ccaggcgcgc catcaaaaga acaaccagtt caagaccgag
     4741 aatttgcggg tgcattcggc ggtcgccggg acggcctcga ctgcgaaccg gaagcgtttc
     4801 acccccaagg cgcgcggttc ggccgggccg atcatcaagc ggaccagcca catcaaaatt
     4861 gtgttgtcag acgaataatt gttatgggcc aaaagactaa tccaatcggt ttgcgcatcg
     4921 ccgtcaacaa agactggcgc tcgaagtggt atgccggaaa aaaagaattc ggcaagctgc
     4981 tgagcgaaga ccgcgacata cgcgatttgc tcaagaaaaa gctcgaatcg gcgtccgtgc
     5041 cgaagatttt gatcgagcgc gccgcgacgc gttgccgcat caccattctc accgcgcgcc
     5101 ccggcgtggt catcggccgc aaaggcgcgg aaatcgacaa gctcaaggaa gaattgagc
//
LOCUS       DASZMM010000352         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1267758, whole genome shotgun sequence.
ACCESSION   DASZMM010000352 DASZMM010000000
VERSION     DASZMM010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267758"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VG754_08555"
     CDS             <1..1182
                     /locus_tag="VG754_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19305.1"
                     /translation="NDWNGAIKDLNEAILLNTNDAWAYMTRGDAYGNKRDYKQAIKDL
                     TESIRLDPKHASSYDSRGVFYAIQKQWDQAIADYDEAVRLGIKGSKVYADRGNAYMAK
                     EQWEKAIADLDERLFYDPKDAASYVSRGRAYGQTRNYKGAFNDFDKALLLNSKNSEAF
                     RYRGLTFYFQHEYDLAIADYTESLRLRPMTVSTLADRGLAYAGKERWDDALDDLYLAI
                     KLDPTNSGCYVNRAFVYSKTHRVENAIDDLNHAIKLNPTNATAFHDLADDYYQQRDYN
                     KAFKELNEGLRLKHDSALLLNLFAWYRAVCPDAAFRNGKEAVHDATKACEISKWKSWS
                     YIDTLAAAYAEIGDFDQALKYEKQASTMEELTEKNIADLKERLELYQTHKPYRENSRS
                     D"
     gene            1296..>3649
                     /locus_tag="VG754_08560"
     CDS             1296..>3649
                     /locus_tag="VG754_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF19306.1"
                     /translation="MLLLGCAVHAEPTEVLPPGFRPVPPGIHALVGGRVVVKPGETLT
                     NATIIIRDGFIEKVGTDVTVPADARVWDMTGLTIYPGFIDAYLTLGTKNADKSPKKSE
                     PAVDLTAGGVKFYGLSQQGSEAGAVSGPVYEVGMVGPERREAQSYQPDPKALEKLREL
                     GFTAGNVVPEKGIFRGTSVFVALSDTDANRAIIKPDVFQHVAFDLEHRRSDVYPESLM
                     GAIAVVRQTIFDAQHYELDQADFAKHPSGRARPAYNLGMEALAPVIEKKMRVVFDPQD
                     ALMVDRSTRLARELDLDFCIVSSGQEWRRPQFVKDAGVPFIVPLNFPTLPKMPTDDDW
                     EQVSLDQLRAWDWAPENAAVLQNQGAEIALTTYGLDDKKDFRKNLRLTLDRGLTESNA
                     LAALTIVPAKLCGLDAMLGTIEPGKQADLTVVDGKGYFDPEAKVRSVWVDGRFYRIPT
                     SEEKEKKEESAKDGSTNSVVAKEVSPEEGEPHQAKEKTEKTGSSEKPKQTADETKSKD
                     KKQADKETKLAKTKELQRRVARSPLEGRGVLTNAPTILVRNATIWTCGPHGILSNADL
                     LVVNGKIAQVGKVTGDFQASQTITIDGTGLHVTPGLIDCHSHSMILGDVNESTLPSSA
                     MVRIGDVVNSESENIYEQLAGGLTEAHLLHGSANPIGGQNCLIKLRDGEPPEGLKFAD
                     ILGSIKFALGENVKQSNWGEKFVTRFPQTRMGVRTFYANRFTAAKEYLKAWDDYKKNG
                     GVPPRRDLELEAIGEILNGTRLIHCHSYRQDEILMLMRLMEEFHV"
BASE COUNT          910 a          919 c         1045 g          775 t
ORIGIN      
        1 aatgactgga acggggcgat caaggatttg aatgaggcca ttctcctcaa taccaatgat
       61 gcatgggctt acatgacccg tggagatgct tatggcaaca aacgggatta caagcaggca
      121 atcaaggact tgaccgaatc catccggctc gatccaaaac atgcgtcgtc ttatgattct
      181 cgcggcgttt tctacgctat tcaaaaacaa tgggaccagg cgatagcaga ttatgatgaa
      241 gccgtccggc tcggtataaa gggttccaag gtttacgccg accgtggaaa cgcatacatg
      301 gcgaaagagc aatgggaaaa ggccattgcg gatttggatg aacggctttt ttatgatcca
      361 aaggacgcgg ccagttacgt ttcgcgcggg cgcgcctatg gccaaacccg aaattacaaa
      421 ggggcattca atgactttga caaagccctt cttctcaatt ccaaaaactc ggaggccttt
      481 cgctatcggg gcctgacctt ttattttcaa catgaatacg atttggccat cgctgattac
      541 actgaatcgt tgcggctgcg cccgatgact gtctccacgc tggctgatcg cggtctggct
      601 tatgcaggaa aagagcgttg ggacgatgcg cttgatgatc tgtatctggc gatcaagctg
      661 gacccgacga attcaggttg ctatgtgaac cgggcattcg tttattcaaa gacgcacaga
      721 gtggagaacg ccatcgatga tttgaatcat gcgatcaaac tgaacccgac aaatgcgact
      781 gcttttcacg atctcgcaga cgactattac caacagagag attataacaa ggcgttcaag
      841 gaattgaatg agggcctgcg gttgaaacat gacagcgcgc ttcttttgaa tctgttcgcc
      901 tggtatcgcg ccgtttgtcc cgatgctgct tttcgaaacg gcaaagaggc tgtccatgat
      961 gcgaccaaag cctgtgaaat ctccaaatgg aaaagctgga gttacatcga cacgctggca
     1021 gcggcttatg ccgaaattgg cgattttgat caggcgctca aatacgagaa gcaggcttcg
     1081 acgatggaag agttgaccga aaaaaatatc gccgatttaa aagagaggct tgagttatat
     1141 cagactcaca aaccgtatcg ggagaattca aggtcggatt gaagaggccg agaaaccttc
     1201 cttgaacaac gttctttccg tgtcataata ctcactcatt gatgcgtctg gttgttccat
     1261 ttatgaatcg gtgctggact ggtttagttg tcgggatgct tctgctgggc tgcgctgttc
     1321 acgcggagcc gacggaagtt ttgccgccgg gatttcggcc cgtgccgccg ggcattcatg
     1381 cgctggtcgg cggacgcgtg gtggtcaaac cgggagaaac gctcaccaac gccaccatca
     1441 tcatccgcga cggattcatt gagaaggtcg gaacggatgt aactgtcccg gcggatgcgc
     1501 gggtttggga catgacgggg cttacgattt atccaggctt cattgatgcc tatctcacgc
     1561 tcggaaccaa gaacgctgac aaatctccaa aaaaatccga gccagccgtt gacctcactg
     1621 ctggcggcgt caaattttac gggctcagcc aacaagggag cgaagcaggc gcagtttccg
     1681 ggccggtgta tgaagttggc atggtcggtc ccgagcggcg cgaagcgcaa agctatcaac
     1741 ctgacccgaa ggcgctggag aaattgcgcg aactcggttt cacggccggg aatgtcgtcc
     1801 cggaaaaagg cattttccgc ggcacgagcg tcttcgtggc gctgtcggac acggacgcca
     1861 atcgcgcaat catcaagccg gatgtttttc agcatgtggc gttcgatctg gagcatcgcc
     1921 gcagcgacgt ctatcccgag tcgttgatgg gcgccatcgc ggtggtccgg caaacgattt
     1981 ttgacgcgca acattacgag ttggaccagg cggattttgc gaagcatcca tcgggccgcg
     2041 cgcgcccggc ctacaatctc ggcatggaag cgctcgcgcc ggtgatcgag aaaaaaatgc
     2101 gagtggtttt cgacccccaa gacgcattga tggtggaccg ctccacgcgc cttgcgcgcg
     2161 agttggacct tgatttttgc atcgtgtcga gcgggcagga atggcgcagg ccgcaatttg
     2221 tgaaagacgc cggcgtgccg ttcatcgtgc cgctgaattt ccccacgctg ccaaagatgc
     2281 cgacggatga cgattgggag caggtgtcgc tcgaccaatt gcgcgcgtgg gattgggcgc
     2341 cggaaaatgc agccgtgctg caaaatcaag gtgctgaaat cgccctgacc acctacgggc
     2401 tcgatgacaa aaaagatttt cgaaaaaatt tgcggctcac cttggaccgc gggctcaccg
     2461 aatcgaacgc actcgccgca ttgacgattg ttcccgcgaa attgtgcgga ttggacgcga
     2521 tgctcggcac catcgaaccg ggcaagcagg cagacctcac tgttgtggat ggcaaaggtt
     2581 attttgatcc cgaagcgaaa gtgcgttcag tgtgggttga cgggcgattt tatcggattc
     2641 cgacatctga ggaaaaggag aagaaagaag agtcggctaa agatggctca acgaactcgg
     2701 tggttgcaaa agaagtttct cccgaagaag gcgagccgca ccaggccaag gagaaaactg
     2761 aaaaaactgg atcatcggaa aaaccaaagc aaaccgctga cgagactaaa tcgaaggaca
     2821 aaaaacaagc ggacaaggaa acgaagctcg ccaagacgaa ggaattgcaa aggcgcgtgg
     2881 cacgctctcc gttggaagga cgcggtgtgt tgaccaatgc gccgacaatt cttgttcgca
     2941 acgcgaccat ttggacttgc gggccgcacg gcatattgtc gaatgcggat ttgctggtgg
     3001 tgaatggcaa aattgcgcag gtcggcaagg tgaccggaga ttttcaggca agccagacca
     3061 tcaccattga cggcaccggc ctgcacgtca cgcccggttt gattgattgc cacagtcaca
     3121 gcatgatttt gggcgacgtc aatgaaagca cgcttccctc ctctgccatg gtgcgcatcg
     3181 gcgatgtcgt caattccgag tccgagaaca tttatgagca gcttgccggc ggattgaccg
     3241 aggcccattt gttgcacggg tcggcaaatc cgatcggcgg gcaaaattgc ctcatcaaac
     3301 tccgcgatgg cgaaccaccc gaagggctga aatttgcgga cattctcggc agcatcaaat
     3361 tcgcgctggg cgaaaacgtg aagcaatcga actggggaga aaagttcgtc acgcgcttcc
     3421 cgcaaacgcg catgggcgtg cgcacgtttt atgcgaaccg tttcaccgcc gcaaaggaat
     3481 atctcaaggc atgggacgat tacaaaaaga atggcggcgt gcctccgcgc cgcgatctcg
     3541 aattggaagc catcggcgaa atcctgaacg ggacgaggct gattcattgc cattcgtatc
     3601 ggcaggacga aatcctgatg ctcatgcgct tgatggagga gtttcacgt
//
LOCUS       DASZMM010000353         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3160405, whole genome shotgun sequence.
ACCESSION   DASZMM010000353 DASZMM010000000
VERSION     DASZMM010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3160405"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..2271)
                     /locus_tag="VG754_08565"
     CDS             complement(40..2271)
                     /locus_tag="VG754_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19307.1"
                     /translation="MRKIWLYLIVSFCCARTLAAQDALIKSDDFEAWHLFETRGEIKA
                     GPDHSAVRLGRTSARIKGTAKSKTILTSPTCEIAPGDELHFEAWARTAKAATNQTAYA
                     GLAFRNRAGHIVQRDYFKARLSENWTLISGDARAPDDAVSVEAHLGYTNAPATVWFAD
                     VTATVTSMFSLSLASDAKPYTGTQEITVKATNRQTNDFNGEISCTVGKQTWKSPAIIP
                     PGESRGFNLPIELTNAGSHHYKLALLIAEDAAVREIKGNFQIAGPMTLFPACPCYLPI
                     GTDGKIRVDAEVNVNPAQRDGLELKVRVLDSAGKILQTVTNDASQAKYAGCDLNVPVD
                     RAATFIVAAELQYHDGNKLASGKTEVRIADVSDSRVTIGPDGYLRIGGKPHFPIGLYS
                     SGNFEEMSKAGFSVTHNYEITTGEAEDAINPNEPRTLELLNKSWANGMRVMLEIPRKA
                     VEKAEWGQVRRFIDTFKHHPALLCWGSEERVARGTTRLQNIEALHALLNELDPDHPFV
                     LGDSRETTARAEKDWPNFFPDSAMDIGTWWWYPIPPHEPVGNQLKPDNLLEPPAWLTS
                     THSKKPLWIAVQAYQQPWKKSRFPTPSEYRCETYLSIIGGVKGLWYYTGYGERDYLGK
                     PAGMLNNPERAHWDHVQKLARELRDFSLVIMSPAAKGKITLLPKNGMVEFASREADGK
                     IYLIAANKSPLPGKARFTSPLFKGRQAHVLYEDHTAQVEGDSLSDDFSPFGVHLYEFM
                     AAK"
BASE COUNT          537 a          668 c          679 g          593 t
ORIGIN      
        1 agtcatcgcg agttgcttga gagaacgaca tcgaacaact catttagctg ccatgaattc
       61 ataaaggtga acgccaaacg gcgaaaagtc atccgacaac gaatctcctt ccacttgcgc
      121 agtgtgatcc tcatacaaaa catgcgcttg gcggcctttg aacaaggggc tggtgaaccg
      181 cgcttttccg ggcagtggag atttatttgc cgcaatcaga taaatcttgc cgtctgcttc
      241 gcgcgacgca aattccacca tcccgttttt cggcaaaaga gtgattttgc ccttcgcagc
      301 aggagacatg atgacgaggc tgaaatcgcg cagttcgcgc gcgagctttt gcacgtggtc
      361 ccaatgcgcc ctctcggggt tgttgagcat gccggcgggt ttgccgagat aatcacgctc
      421 gccatatccc gtgtaatacc aaaggccttt cacgccgccg atgatgctca agtaagtttc
      481 gcagcgatat tccgagggcg tggggaaacg ggattttttc cacggttgct ggtaggcttg
      541 gactgcgatc cagagcggct ttttggaatg ggtggaagtg agccaagcag gcggctcgag
      601 caaattgtcc ggcttcaatt gattgccaac cggctcatgg ggcggaatcg gataccacca
      661 ccaggttcca atgtccattg ccgaatcggg aaagaaattg ggccaatctt tttcggccct
      721 tgcagttgtc tcccgcgaat cgccgagaac gaacggatgg tcgggatcaa gctcgttcaa
      781 cagggcatga agcgcctcga tgttttgcag ccgcgtggtt ccgcgcgcga cgcgttcttc
      841 cgagccccag cagagcaggg cgggatgatg cttgaaagtg tcgataaagc gccgcacctg
      901 gccccattcg gccttttcaa cggccttgcg cggaatctcg agcatcacac gcattccatt
      961 ggcccaggat ttattgagca attccaaggt gcgcggctca tttggattga tggcgtcttc
     1021 cgcctcgccg gtcgtgatct cgtaattgtg cgtgacactg aaaccggcct tgctcatctc
     1081 ctcaaaattt ccgctgctat acaaaccgat cggaaaatgc ggcttgcctc cgatgcgcag
     1141 gtaaccgtcc ggccctattg tcactcgcga atcactgaca tcggcgatgc gaacttccgt
     1201 cttgccgctg gcgagtttat tcccgtcgtg atattgcagc tccgcggcga ctataaatgt
     1261 ggcggccctg tcaaccggca cattcaaatc gcaaccggca tattttgcct gcgaagcgtc
     1321 gttagtcacg gtctgcaaaa ttttgcctgc ggaatcaagc acacggactt tcagttccaa
     1381 tccgtcgcgc tgcgctggat tcacatttac ctcagcatcc acccggatct ttccgtcagt
     1441 tccgatggga agatagcagg gacaagcggg aaaaagtgtc ataggcccgg cgatttgaaa
     1501 attgcccttt atctcgcgca cggccgcgtc ttccgcgatg agcaatgcca gtttgtagtg
     1561 atgcgaaccg gcgttggtca gttcgatcgg caaattgaat ccccggcttt ctccgggagg
     1621 aatgatggcc ggacttttcc aggtttgctt gccgacggtg caagagattt cgccgttgaa
     1681 gtcgttcgtt tgccggttgg ttgctttgac agtgatctcc tgtgtgccag tgtagggctt
     1741 cgcgtcactg gcaagcgaaa gcgaaaacat gctggtgacg gtcgccgtga catcggcaaa
     1801 ccaaaccgtc gcgggggcgt tggtgtaacc cagatgcgcc tcgaccgaaa cagcatcgtc
     1861 gggagcgcgt gcgtcgccgg aaatcaaagt ccaattttcg gaaagcctgg ctttaaaata
     1921 atcgcgttgc acgatgtgtc ccgcgcgatt cctgaatgca agccctgcat acgccgtctg
     1981 gttggtcgca gctttcgctg ttcgcgccca ggcttcgaaa tgcaattcgt cgcccggcgc
     2041 gatttcacag gttggacttg tcaaaatggt cttcgatttt gcggtgccct tgatccgggc
     2101 ggaagttctg ccgaggcgga cggcggagtg gtccggccct gcctttattt caccacgcgt
     2161 ttcaaacaga tgccaggctt cgaagtcgtc agatttgatg agcgcgtcct gcgccgccaa
     2221 agttcgcgcg caacaaaaac tgacgatgag atacaaccag attttccgca tggcgagtta
     2281 caggatggat taaatccaat tattaatgaa gtaaaattgg acaaacaact aaaactattt
     2341 tagggctatg cacttgatgc cccttggtgg cctttgccat gccgcgacac gcttgcccgc
     2401 aaatggcacc agattacttc tggagcgctt tttccgaaaa aagaccggaa atttgcggtt
     2461 gctctttgcg agatgcc
//
LOCUS       DASZMM010000354        10337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4815406, whole genome shotgun sequence.
ACCESSION   DASZMM010000354 DASZMM010000000
VERSION     DASZMM010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4815406"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2568)
                     /locus_tag="VG754_08570"
     CDS             complement(<1..2568)
                     /locus_tag="VG754_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWF19308.1"
                     /translation="MKHGSLYTVSIALIVAVGGFLLGFDATVISGAVPFIKKFFQLSG
                     TGGDLKLGWAVSCLGWGALGGNALAGLLSDAFGRKKVLMLTAVLFTVSALLSATTNDF
                     AIFVISRILGGVAVGGAILIAPVYIAEIAPSALRGSLVSFNQLMIVIGISASFFSNYY
                     LLRLGENCWRWMLGVEAIPAALYFVLLFFVPESPRWLYGKGREEQAREIFTKVAGAAQ
                     AEEELSRIRQNAAEHVAGVGAGALFSRRMRFVMFIALTIAFFQQITGINAIFYYLPSI
                     FSKAGGGMDSAFKQAVIVGLVNLGMTFVAIKWIDRLGRKPLLVIGTAGISLSLLTCAA
                     AFHNSNYQLTEKSFASLRAGKVPEDIISDLQKGGPQVFATENEFLSAMDSKLGADRLA
                     PFHDALTSASLNIRASLVLFAIIGFVASFAISLGPVMWVLLSEIFPNAYRGTAISIVG
                     FWNSVVSATVTMIFPWELSHLGTAGTFFGYGLLGLAALVFVLMAIPETKGKSLEELEH
                     FFVRGVTAIDTTSPAPQPKYVSTKLPLVLFIALAFAGSLVASENAKPGGPAKVEVRQT
                     DGHWQLLVNQRPFYIKGVGLEFGDQEKLAAAGGNSFRTWRTEKGRQVLDLAQKNGLYV
                     TMGLDLSKERHGFDYSDPAAVAKQLEGIKAQVLQLKDHPALLMWDIGNELNLNSHNPK
                     VWDAVNEISKMIHQIDPNHPTTTSLAGVSKNVVEQIKSRAPDLDLLCFQSYADIVNLQ
                     REIRESGWSGPYAVTEWGATGHWECGKTAWGAPIEDDSSVKADFFRRRYELAIAADTK
                     NCLGSYAFLWGQKQERTPTWYGMFLPAGETTETVDVLQHLWTGQWPELRSPQVKGFWL
                     "
     gene            complement(2623..3384)
                     /locus_tag="VG754_08575"
     CDS             complement(2623..3384)
                     /locus_tag="VG754_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF19309.1"
                     /translation="MHNQKRSAREAFTLIELLVVIAIIAILAGLLLPALARAKAKARA
                     ISCTSNNKQIGLAMMMYVTDNNDYLTPLNDRNFASHTTNWWFRYLDNGNYITTSLITN
                     NVWHDPEVQPQDIQAGTVAYYDAPCEGYGPLEDTVNPANCPVRYNWDLSGKVQGSYKL
                     GDLDRASQLWMIGDVGTPKTGGGINVEPTSYYTDITVIKPVPNFGWTPVPSYKQAACR
                     HSGRAVFSFCDGHVESWPWLNLDTDVNDVFGVKSL"
     gene            complement(3573..5576)
                     /locus_tag="VG754_08580"
     CDS             complement(3573..5576)
                     /locus_tag="VG754_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19310.1"
                     /translation="MKTRISNLMVLFLALCAFVCGTVRVNSQVLVTVDPNQNWIGYMN
                     VFQLPADGGAYEFGSTWGTADLRGVFGTTNLVLSPCTNVWETQDTAWVKADGMTPNDN
                     MDANFYVQNDSLANTNLIFVGKCVSNTITNAPEPLTGVSYTSVAFIKIFDSGYGLLGS
                     ATSNLVGGQSFSISMDTTGAAHVQYGFETIGPDANPATVTNLGNVVIANAAPSKIVTV
                     DPSQNWIGYMNVFALPATGGGYQFGSAWGTGALQAAFSGTNFLTLAPDTNVWETNDTY
                     WVQADGVTPNKNMDASMYVQNDNLVNTNLVLLGTCLTNTLSAAPEPHTGISYTSVAFI
                     KTFDGSFNLLGSAASPLLAAGQPFAISLNTTGATHVQYGFETIGPDASPATASSLGDV
                     VLAAQAPPAAPVVPTNNAPTPSHPANKVLSMYNSSGVYTNHPIERWLAGWSGAGENLY
                     TISPTGRVVLKYSNLQYAGVEFYNNDPTLGAGGDNVGGPTNYAINATGYDTFHVDVWT
                     PDANQLGIQLVSINPTEAAQVDYLPASGVITNDGWISFDIPLSTFQANNPNTDFTDLQ
                     QMLWIDNQAGGGYTGGTFYIDNVYFYSSTSVTRPTLAATVSGGIIQLSFPTQNGFNYT
                     VQYRTNLTDSGWQTLSTVSGNGSTQSIPDSANQKSRFYRLYIH"
     gene            complement(5717..6352)
                     /locus_tag="VG754_08585"
     CDS             complement(5717..6352)
                     /locus_tag="VG754_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF19311.1"
                     /translation="MIKVGIVEDNKTVREGFDTLLNRTPGFQCVCACETVADALKKIP
                     KARPDVVLMDIQLPDATGVECTAKLKEMLPSMQIVIVTVYEDSERIFQALRAGACGYL
                     LKRATPEKVIAAVREAKEGGVPMTPEIARKVIGQFRQQATTESQVEDLSNREREVLEL
                     VMHGLGNKAIADRLGVTVAAVKWHLQHIYEKLHVHSRTEAALKFKQGQGLD"
     gene            complement(6349..8394)
                     /locus_tag="VG754_08590"
     CDS             complement(6349..8394)
                     /locus_tag="VG754_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19312.1"
                     /translation="MLFLGCFQGVEILAEELHTVAAIRDLTFEQTQQKIPVRLKGVAT
                     FFDERLYSHFIQDDTAGIYLQFPTNVGPPALNPGQLVEVTGVCSPGEYAPVVVVDRIE
                     AVGQAPLPAPKIVTYEELASGVEDSQYIQFTGIVRSAKLQDDSQYNLVEIAAGGGRLL
                     VYAKNLPVKKPEELVDSTVRVRGVCSTKFNHKRQLFAIRVMVPRPDDLEIMLIPPTDP
                     FEIPARPIGSLLQFAPQESYGHRVKVQGTVIYYMPGESIFLQDGEQGVEIQTEERNPL
                     QLGDRVEVLGFVSQGEYTPLLQNATYRKISPGNPPMPAVLTADEALKGNHDCRLIQIA
                     AKLLDRTQHGAERSLILQADNVIFYAYLRQAEGEKTFANLQNGSSVLVTGVCQIDPGE
                     WAAGEAWRAKGFKIQMRSAADVLLVKAPPWWTVKKVVWMAGALGFAMLAAFAWVGVLR
                     REVAERTHQLETQIRERQRAERRREIEQERARVAHDLHDDLGAGLTEVNMLSSLVKSS
                     TTSSEEKERYLDDLTETARRMVTSLDEIVWAVNPRNDTIASLASYFGSYAQRLLDLAG
                     IACGLDIAEDLPEHPLDPKFRQELFLAFKEALTNVVRHSHATKVWLRIFVRKRFLVVE
                     VADNGKGFDPSECQAGSDGLNNMKERLKSLGGDREIASSEKTGTTVQFSAPLPERLL"
     gene            8889..>10337
                     /locus_tag="VG754_08595"
     CDS             8889..>10337
                     /locus_tag="VG754_08595"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19313.1"
                     /translation="MKKTISIPAPRNLAQLLAVIFCMASIFGARANNLVQEFYLPMPE
                     AQIAQANNSIISGTGSTISSTFSIIVTGSGTVIYYDQWEDGYETDLANPTQSTTQIWG
                     DGNDAHGIPPGFAHNPLGLPAGTVITLTNNVTQPRNPSQILWDARDRIGANKALIISR
                     AGWPAQTGPVFAGAVSVLSTMDYGTNYISPVGQDLTNINLMKYVGLFIMAAQDNTAVT
                     IDPNGNGVGTTNIVLNQGESYLVNGGVKTGGRVTATKPIQADLIIGHVGAAYASDWFT
                     LYPVTAWSGSYYTPVGSAAKVSEPAYVYIYNPNTNAITINYNTKVGSGSVSVPASNGV
                     HQFQMPVSSGASFASAGGQSFYALCTVAANNASDTPWNWGFTLVPQGALTTAATVGWA
                     PGSADGTVDGSPVWVTALTNTTIYVDYHGDTNGPRIDPNGNHYDTNFSVTALQSLKIF
                     DPTKNQTGMKIYTVDGTLITATWGEDADTAQPG"
BASE COUNT         2339 a         2927 c         2719 g         2352 t
ORIGIN      
        1 cagccaaaaa cctttcactt gcgggctgcg aagttccggc cactgtcccg tccacagatg
       61 ctgcagcaca tccacggtct cggtcgtttc acccgctggc aggaacatgc cgtaccacgt
      121 gggcgtccgt tcctgttttt gtccccacag aaaagcgtag gagccaaggc agttcttcgt
      181 gtccgccgcg atggcgagtt cgtaacgcct gcggaagaaa tcagctttca ccgaactgtc
      241 atcctcgatg ggcgcgcccc acgcggtttt gccgcattcc caatgacctg ttgcgcccca
      301 ttccgtgacc gcatacggtc cgctccagcc gctctcgcgg atttctcgct ggagattcac
      361 gatgtcggca taggattgga aacaaagcag atcaaggtcg ggcgcgcgac tcttgatttg
      421 ttccacaacg ttcttgctca cgcccgcgag cgaagtggtc gtcggatgat tcgggtcaat
      481 ctgatggatc atcttcgaaa tttcattgac cgcgtcccag acttttgggt tgtgcgaatt
      541 caaatttaat tcattgccga tgtcccacat gagcagggca gggtggtctt tcagttgcaa
      601 tacttgcgct ttgattcctt ccaattgctt cgcgacggca gccgggtcgg aataatcgaa
      661 tccatggcgc tccttggaga gatccagccc catcgtcaca tacaaaccat ttttctgcgc
      721 gaggtccaga acctgccgcc ctttttccgt tcgccaggtg cgaaatgaat taccacctgc
      781 cgcggccagt ttttcctgat cgccaaattc cagtcccacg cctttgatgt aaaatggccg
      841 ctgattcacc aagagttgcc agtgaccatc agtctggcgc acttccactt ttgcggggcc
      901 gccgggtttt gcgttttcac ttgcgacaag gctgccggcg aatgcaaggg cgataaaaag
      961 taccaacggt aatttcgtcg aaacgtattt tggctgggga gcagggctgg tcgtatcgat
     1021 tgcggttacg ccccgaacaa aaaaatgctc cagttcttca agcgatttgc cttttgtctc
     1081 tggaatggcc atcaacacaa acaccaacgc cgcaagtccc aacaagccat agccgaagaa
     1141 cgtccccgcc gtgccgagat gggacaattc ccagggaaaa atcatcgtca cagtcgcgct
     1201 cacgaccgaa ttccaaaagc cgacgatgga gatcgccgtt ccgcgatagg cattgggaaa
     1261 tatttccgag agcagcaccc acatcaccgg cccgagcgaa atcgcaaacg acgcgacgaa
     1321 cccgatgatg gcaaagagaa ccagcgaagc gcggatgttc agcgacgcgg acgtcaacgc
     1381 atcgtgaaac ggagcgagcc ggtccgcgcc gagcttgctg tccatggcgc tcagaaattc
     1441 attttccgtg gcgaaaactt gcgggccgcc cttttgcagg tcgctgatga tgtcttcagg
     1501 cactttgccg gcgcgcagag acgcgaatga tttttcggtc aattgataat ttgaattgtg
     1561 gaacgcggcg gcacaggtca ggagtgaaag gctgatgccc gccgtgccga tgaccagcag
     1621 cggtttgcgt cccaagcggt caatccattt gatcgccacg aatgtcatgc ccaaattgac
     1681 caaacccaca atgacggctt gcttgaatgc ggaatccatg ccgccacctg ctttcgagaa
     1741 aatgctcggc agataataaa aaatcgcatt gatgccggtg atctgttgaa aaaatgcgat
     1801 cgtgagggcg atgaacatca caaatcgcat cctccggctg aacaacgcgc cagcgccaac
     1861 tccagctaca tgttccgcag cgttttgacg gattctgctc agctcttcct ctgcttgggc
     1921 agcgccggcc accttcgtaa aaatctcgcg cgcctgttct tcacggccct tcccatagag
     1981 ccagcgcggg ctttcgggca caaaaaacag aagcacgaaa taaagcgccg ccgggatggc
     2041 ctcgaccccg agcatccagc gccagcaatt ttcgccaagt ctcaggaggt agtaatttga
     2101 aaaaaacgaa gccgagatgc cgatgacgat catgagttgg ttgaaggaaa cgaggctgcc
     2161 gcgcaaggcg gacggagcga tttccgcgat gtaaaccggc gcgatgagta tcgcgccacc
     2221 cactgcaacg ccgccaagaa tccgggaaat cacgaaaata gcgaaatcgt tcgttgtcgc
     2281 ggaaagcaag gctgagacgg tgaacaacac cgccgtcaac atcagaactt tcttgcggcc
     2341 gaatgcatcg ctcaacaagc ccgccaacgc gtttccgcca agcgcgcccc aaccgagaca
     2401 gctcaccgcc cagccaagtt tgaggtcgcc gcccgtgccg ctgagttgga aaaacttttt
     2461 gatgaacggc acagcgcctg aaatgaccgt ggcatcaaag cccaaaagaa atccgcccac
     2521 cgccacaatc aacgcaatgg aaacggtata taaggaaccg tgcttcatat cttgaatctt
     2581 ttgtcgtccg cgctgaactt cgatgggacg gcgaatcggg ttttacaagg atttcacgcc
     2641 aaaaacatca ttgacgtcag tgtctaaatt cagccatggc cagctttcga catgcccatc
     2701 gcaaaaagaa aaaacggcgc gtccactgtg cctgcaagcc gcctgtttat aggaaggaac
     2761 aggcgtccag ccaaagttag gtaccggctt gattaccgtg atgtccgtgt aatagctggt
     2821 cggttctacg tttatgccgc cgccggtttt tggagtcccg acgtcaccta tcatccaaag
     2881 ctggctggcc cgatcaaggt ccccgagctt gtaactgccc tgcaccttgc cgctgaggtc
     2941 ccaattgtaa cgaaccgggc aattggcggg attcaccgtg tcttcaagcg gcccataacc
     3001 ctcgcaagga gcgtcataat aagcgaccgt gcctgcctgg atgtcctgcg gttgcacctc
     3061 gggatcatgc cagacgttat tcgtgatcaa actcgtggtg atgtaatttc cgttgtcgag
     3121 atacctgaac caccagttcg tcgtgtgcga cgcgaagttt ctatcattca aaggcgtcaa
     3181 gtaatcgttg ttgtcggtca cgtacatcat catggcgagc ccgatctgct tgttattgct
     3241 tgtgcaggaa atggcccttg cctttgcctt ggcccttgcc aatgccggca acaacaatcc
     3301 cgcgaggatt gcaatgatgg cgatgaccac cagcaattca atcaatgtga acgcctccct
     3361 ggcggaccgc ttttggttat gcatactttt gctaatttga actctcataa acgtcatgag
     3421 ttgtgacagc ccattttgtt ggtcggcagc gcacagccaa ccccggcttg tgaccgaaag
     3481 cgtgacagtc ccgggattcc ctgaaggcgg tgctgtttgt ttgctttcgt attcatcatt
     3541 gccagatttg gctgcgccag ttgtttttcc gattaatgga tatacagccg atagaaccgg
     3601 ctcttctggt tggctgaatc aggaatcgat tgggttgacc catttccgga gacagttgac
     3661 aaggtctgcc aacccgaatc cgtcaggttg gttctgtatt gcacggtgta gttgaaaccg
     3721 ttttgcgtcg ggaaggacaa ctggatgatc ccacccgaaa ccgtggcggc gagggtcggc
     3781 cgtgtcacgc ttgtgctgct gtagaaataa acattatcaa tatagaacgt gccgccggtg
     3841 taaccaccgc cggcttgatt atcaatccac agcatttgtt gaaggtcggt gaaatcggtg
     3901 ttcggattat ttgcttggaa tgtgctcaag ggaatatcaa agctgatcca gccgtcgttt
     3961 gtgataacgc cgctggccgg gagataatcc acttgcgcgg cttccgttgg attaatgctc
     4021 accaattgga tgccgagttg gttggcgtcg ggcgtccaga cgtccacatg gaatgtgtcg
     4081 taaccggtgg cattgatcgc ataattcgtc ggtccgccca cgttgtcgcc gcctgcgccg
     4141 agcgtgggat cgttgttgta gaactccaca ccggcgtatt ggagattgga atatttcagg
     4201 acgacgcggc cggtcggcga aatggtataa agattttctc cagccccgct ccaaccggcg
     4261 agccaccgtt caatcgggtg atttgtataa acgccgctgc tgttgtacat cgacaatacc
     4321 ttgttcgccg ggtgggaagg agtcggcgca ttgttggtcg gcaccacggg cgcagcaggc
     4381 ggagcttgcg ctgccaaaac cacatcgccc aacgaagacg cggtcgcggg actcgcatca
     4441 gggccgatgg tttcaaatcc atattggaca tgcgtcgcac cggtggtgtt gaggctgatg
     4501 gcaaacggtt ggccagcggc caacagcggc gacgccgcgc tgccaagcag gttgaagctg
     4561 ccatcgaatg tcttgatgaa cgccacagag gtgtaactaa tgccggtgtg cggttcaggc
     4621 gcggcgctga gggtattcgt caaacaagta ccgaggagta ccagattagt gtttaccagg
     4681 ttgtcatttt gcacatacat gctggcatcc atgttcttat tgggagtgac tccatcggcc
     4741 tgcacccaat acgtatcgtt ggtctcccaa acattggtgt caggggccaa ggtgagaaag
     4801 ttggttccgc taaaggcagc ttgcagtgcg cctgtccccc aagcgctgcc aaattgatag
     4861 ccacccccgg tggccggcaa tgcaaaaacg ttcatgtaac caatccaatt ctggcttgga
     4921 tcaacggtca caattttcga tggcgcggca ttcgcgatca cgacgttgcc aaggttggtg
     4981 acggttgcgg gattggcatc cggcccgatg gtttcaaatc cgtattgcac atgcgccgct
     5041 ccggtcgtgt ccatgctgat ggaaaacgac tgcccgccca ccagattgct cgtggcggaa
     5101 ccgagcagac catagccgga atcaaatatt ttgatgaaag ccacggaagt ataggaaaca
     5161 ccggtcagcg gttcgggcgc gttggttatc gtgttgctca cgcattttcc gacgaagatc
     5221 aggttcgtgt tcgccaagct gtcgttctgg acgtagaagt tggcatccat gttatcattg
     5281 ggagtcatgc catcggcttt gacccaagct gtatcttgcg tctcccaaac attcgtgcag
     5341 gggctcaaaa ccaggtttgt tgtgccaaaa acccctcgca ggtcggcggt tccccaggta
     5401 ctgccgaatt cataagcgcc gccgtccgcg ggcaattgga aaacgttcat gtatccgatc
     5461 caattctgat tcggatcgac ggtgacgagg acttgcgaat tgacccggac cgtgccgcaa
     5521 acgaaggcgc acaaggccag gaaaagaacc atcaggttgg agattcgtgt tttcatggtc
     5581 gtagaatggt cgaggggtga gcgatacgtg aagagactcg cgtcccggtt tgaattggtt
     5641 ttcatagggt tcgccctaac cttagctggc ttcccttctg taccaagcta gccaagcggc
     5701 tagtaaaacg ggctgtttaa tcaagaccct gtccctgctt gaatttcagc gcggcttctg
     5761 tccgtgaatg gacgtgcaac ttctcgtaaa tatgttgcag atgccatttt acagcagcca
     5821 ccgtcacgcc cagccggtcg gcaatcgcct tgttgcccag gccatgcatc accaattcca
     5881 agacttcacg ttcgcggttg gacaagtctt ccacctgcga ctcggtcgtc gcttgctgac
     5941 gaaattggcc gatcaccttg cgcgcgattt ccggcgtcat cggcacaccg ccttctttcg
     6001 cttcgcgaac cgcggcgatg actttttccg gcgttgcccg cttcaacaaa tatccacaag
     6061 ccccggcacg caacgcctgg aagatgcgct cggaatcttc atacaccgtc acgatcacga
     6121 tttgcatgga aggaagcatc tccttgagtt tggcggtgca ttccactccc gttgcgtcgg
     6181 gcaattgaat gtccatcaag acaacatctg gtcgcgcctt gggaattttt ttcagagcat
     6241 ccgcgacggt ttcacaggcg cagacacatt gaaaacccgg cgtgcggttc aacaacgtgt
     6301 cgaatccttc acgcacggtt ttgttgtcct cgacaattcc aactttgatc atagcaatct
     6361 ttcggggaga ggcgcggaga actgaactgt tgttcccgtt ttttcgctgc tcgcaatttc
     6421 gcgatcgccg ccaagggatt tcaaacgctc tttcatgtta ttcaatccgt cgctcccagc
     6481 ctggcattcg gaagggtcga atcccttgcc gttgtcagcc acttcgacaa caaggaaacg
     6541 tttgcgcacg aagatgcgaa gccagacctt cgtggcgtgc gaatgccgga cgacgtttgt
     6601 gagcgcttct ttgaacgcaa ggaaaagttc ctggcgaaat ttcgggtcca gcggatgctc
     6661 cggcaaatct tcggcgatgt ccaagccgca ggcgatgccc gcgagatcca gcaggcgttg
     6721 cgcatacgat ccgaagtaac tcgcaagcga tgcgatggtg tcgttgcgcg gattcaccgc
     6781 ccagacgatt tcatccagcg aagtgaccat tctgcgggcc gtttcggtca agtcgtcaag
     6841 gtaacgctct ttctcctcgg aagatgtcgt cgaacttttc acgagcgaac tcagcatgtt
     6901 gacttccgtg agtcccgccc cgaggtcgtc gtgcaaatcg tgcgccacgc gcgcgcgttc
     6961 ctgctcgatt tcgcgccgcc gttcggcgcg ttgacgctcg cggatttgtg tctccaactg
     7021 gtgtgtgcgc tccgcgacct cccggcgcaa cactcccacc catgcgaacg ccgccaacat
     7081 cgcgaaacca agagcgcccg ccatccacac gacttttttc accgtccacc aaggcggcgc
     7141 tttcaccaaa aggacgtcgg ctgccgaacg catctggatc ttgaaacctt tcgcgcgcca
     7201 ggcttcgcct gcggcccatt cgcccggatc aatctggcaa acgcccgtca ccagcacgct
     7261 gctgccattt tggaggttcg cgaaagtttt ttctccctcg gcttgtctga gatatgcgta
     7321 aaaaatgacg ttgtcggcct gaaggatcaa agagcgttcc gcgccatgct gcgtgcggtc
     7381 cagcaacttc gcggcgatct ggatcaacct gcaatcatgg tttcctttca gcgcttcatc
     7441 ggcagttaaa acagcgggca tcggcggatt gccgggagaa atttttctat acgtggcatt
     7501 ttgcagcagc ggcgtgtatt caccctggct gacgaaccca agcacttcca cgcggtcgcc
     7561 aagctgcagc ggattgcgtt cttcggtctg gatttcgacc ccttgttcgc catcctgaag
     7621 aaagatggat tcaccgggca tgtaataaat aacggtgcct tggaccttga cccgatggcc
     7681 atagctttcc tggggcgcaa actgcagcaa gctgccaatg ggccgcgccg gaatctcaaa
     7741 aggatctgtt ggaggaatga gcatgatttc caaatcgtct gggcgcggca ccatcacacg
     7801 gatggcgaac aattgccttt tgtgattgaa cttggtcgag caaacaccgc gcacgcgcac
     7861 ggtgctgtcc accagttcct ccggtttctt cacaggcaaa ttcttcgcgt aaacgagcaa
     7921 ccgccctcca cccgcggcga tttcgaccag gttgtattgc gaatcgtcct gcagcttggc
     7981 cgacctcacg atgccggtga attggatgta ctggctgtct tccactccac tggccaattc
     8041 ttcataagtc actatttttg gcgcgggcaa aggagcttga cccacggcct cgatgcggtc
     8101 cacgaccacc accggagcat attcgccggg gctgcaaacg ccggtcacct cgaccaactg
     8161 gccgggattc agggcagggg gaccgacatt cgtcggaaat tgcagataaa ttcccgccgt
     8221 gtcgtcttga atgaaatggg aatacagccg ttcatcgaaa aaagtggcca cacctttcaa
     8281 gcgcaccggg attttctgct gagtctgttc aaacgtcaga tcgcggattg ctgccacggt
     8341 gtgcagttcc tcggcgagaa tttcaactcc ttgaaaacac ccaagaaaca acacaaatag
     8401 aatcagcctg ggcccaaacc gcagaccttg aaacaaaaaa tttagcttcg tcgccatctt
     8461 cgccaaggcg gaaacgccat gatgtccttt tcgcctgggc tcacccggct tatgtcgcat
     8521 acagccccaa ttgtcaatca aactgacaat gcaagggcaa tgggttctcc acaatttttt
     8581 ggcgatgctg tgcctttttt ctcaataaga gttgttgtct atttcttcat taagccgccc
     8641 attaacgtaa ggaaacaaga cacttgggta aaattccctt gtaagtcgtc gtacaccagt
     8701 tgttgtagtc actcgcttta cacacatctg gttgtgtcag accacacttc caatttttct
     8761 tgcattatgc ggcacgtggg ttgctcaggg ggtcttgtat tcggatttaa atgacacctg
     8821 tgacaaataa tagtcaatct gcgggacgaa ataaccaatg ggttatcgcg tcctgatttc
     8881 ctgccgcaat gaaaaaaaca atcagcatcc ccgctccccg aaatctggcc cagttactgg
     8941 ccgtcatctt ttgcatggcc tccatcttcg gcgcgcgcgc gaacaatctc gtgcaggaat
     9001 tttatctgcc catgcccgaa gcgcagattg cccaagccaa caactccatc atcagcggca
     9061 ccggttccac catcagttcc acgttttcaa ttatcgtcac cggcagcggc accgtgattt
     9121 attatgatca atgggaggac ggttacgaaa ccgacctcgc caaccccacg caatcaacga
     9181 cacaaatttg gggcgatggc aacgacgccc acggaattcc gcctgggttc gcgcacaatc
     9241 ctctgggctt gcccgccgga acggtcatca cgctgacgaa caatgtcact cagccgcgca
     9301 atccttcgca aattttatgg gatgcccgcg accgcatcgg cgcgaacaaa gcgctgatca
     9361 tttcccgcgc aggttggcct gcacaaaccg ggccggtgtt cgcgggagcc gtgagcgttt
     9421 tgtcaacgat ggattacgga acgaattaca tcagccccgt cggccaggac ctcaccaaca
     9481 tcaacctgat gaaatacgtc ggcctgttca ttatggccgc gcaggacaac actgcggtga
     9541 cgattgatcc caacggcaac ggcgtgggca cgaccaacat agtgttgaac cagggtgaat
     9601 cttatcttgt gaatggcggc gtcaaaaccg gcggccgcgt cacggcaacc aagccaatcc
     9661 aggctgacct catcatcggc cacgtcggcg cggcttacgc aagcgactgg ttcactttgt
     9721 atccggtgac agcatggagc ggcagttact acacaccggt cggttcggcg gcgaaagttt
     9781 cggaacctgc ctatgtttat atttacaatc ccaacacgaa cgccatcacc atcaattaca
     9841 atacaaaggt gggttccggc agtgtttcag tgccggcctc aaacggcgtt catcaattcc
     9901 agatgcctgt cagttccggc gcaagtttcg cgagtgcggg cggccaaagt ttttacgccc
     9961 tttgcacggt agctgcgaac aacgcatccg acaccccttg gaattggggt ttcacattgg
    10021 tgccgcaagg cgcgttgacg accgccgcga ccgttggttg ggcgccggga agcgcggacg
    10081 gcactgttga cggcagcccg gtttgggtga cagcgctgac gaatacgaca atctatgtgg
    10141 attatcacgg agataccaat ggcccccgaa tcgatccgaa tggaaatcat tacgatacga
    10201 atttctccgt gaccgcctta caatcgttga aaattttcga cccgaccaaa aaccagaccg
    10261 gcatgaaaat ttacacagtg gacggcacct tgatcaccgc cacatggggc gaagatgccg
    10321 acaccgcgca accgggt
//
LOCUS       DASZMM010000355         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1790493, whole genome shotgun sequence.
ACCESSION   DASZMM010000355 DASZMM010000000
VERSION     DASZMM010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790493"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1512)
                     /gene="lpdA"
                     /locus_tag="VG754_08600"
     CDS             complement(94..1512)
                     /gene="lpdA"
                     /locus_tag="VG754_08600"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWF19314.1"
                     /translation="MESIKTEIVVVGGGPGGYTAAFFAADLGKKVVMIEKDKRLGGVC
                     LNRGCIPSKALLNVAHTISDARESAHRGIYFEPPKVDVAKLRAWKESVVQKLCGGIAM
                     LAQKRGVQVIYGRAHFEGSQSLRVETEQGQQFVNYDKAIVATGSKPAMPKTFDLGNPR
                     IMTSTEALETEDVPENLLVVGGGYIGMELGTVYATLGSKVVLVEALDSILMGADPDLA
                     RPVMAYAKKAFKEVRLKAKVVKMSTSGKQIKVEMELDGKKIEEFYDRVLVAVGRVPNG
                     SDLGLENTKATLDEKGFIKVDPQQRTTDENIYAIGDIAGGILLAHKAFREGRIAVEAI
                     TGESTSPEKFIVPAVVFTDPEVAWCGLTEAEAKAQGREVEVAKFPWGASGRAMTFDRP
                     DGLTKLIIDPQTERILGVGIVGHGAGELIAEGVVAVEMGATVRDLALSVHPHPTLSET
                     IMEAAEVFYGQATHTLSKKRAE"
     gene            complement(1531..>1804)
                     /locus_tag="VG754_08605"
     CDS             complement(1531..>1804)
                     /locus_tag="VG754_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWF19315.1"
                     /translation="TFTISNQGGIGGAHFTPIVNKPEVAILGLGKGAMKPVVKKDKIE
                     PRMMLPIGLSYDHRVIDGGGAARFTVDLLQALENFNEQDVKSGVME"
BASE COUNT          375 a          569 c          452 g          408 t
ORIGIN      
        1 gcgctaccaa gctacgctac gtcccgaacc atccggcctg tctgacgaca ggcaggaaca
       61 aaatattatg gcgttgatgc cggaattcaa caactattcc gcgcgtttct tcgataatgt
      121 gtgcgtcgcc tgtccgtaaa agacctcggc cgcctccatg atggtctcag aaagcgtcgg
      181 gtgcggatgc acgctcaacg caaggtccct gacggtcgca cccatctcca cggccacaac
      241 gccttcggca atcaactcgc ccgcaccatg cccgacaatt ccaacgccca aaatacgctc
      301 ggtctgcggg tcaatgataa gcttggtcag cccatcgggg cggtcaaatg tcatcgcgcg
      361 tccagatgcg ccccaaggga atttcgcgac ctcgacttca cggccttgcg ccttcgcctc
      421 ggcttcggtc aatccgcacc aagccacttc gggatcggtg aacaccaccg ccgggacgat
      481 gaatttttcc ggcgaagtgg attccccggt gatggcttca acggcgatgc ggccttcccg
      541 aaacgccttg tgcgcgagaa gaattccgcc ggcaatgtcg ccgatggcat aaatattttc
      601 atccgtggtg cgttgctgcg ggtcaacttt gatgaacccc ttttcgtcga gggtcgcctt
      661 ggtattttcg aggccgaggt cgctgccgtt cggaacacgg ccaacagcca ccaggacgcg
      721 atcgtaaaat tcctcaatct ttttgccatc cagttccatc tcgaccttga tctgctttcc
      781 gctggtggac atcttgacga ccttggcctt gagacgcact tccttgaacg cttttttcgc
      841 gtaggccatc accggccgcg ccaaatcggg gtcagcgccc atcaaaatgg aatccaacgc
      901 ttcgacgaga accactttgc tgcccagcgt ggcatagacc gttccaagtt ccatcccgat
      961 gtaaccgccg ccgacgacca gcagattttc agggacgtct tccgtctcca atgcctcggt
     1021 ggaagtcatg atgcggggat tgcccaaatc gaatgtcttg ggcatcgcag gcttcgagcc
     1081 agtcgcgacg atggccttgt cgtaattgac gaattgctgg ccttgctcgg tttcaacgcg
     1141 caagctttgc gagccttcaa aatgggcgcg cccataaatc acctgcacgc cgcgcttctg
     1201 cgcgagcatc gcgatgccgc cgcaaagttt ttgcacgacg gattccttcc atgcgcgcaa
     1261 cttggccaca tccaccttgg gcggttcgaa ataaatgccg cgatgcgccg attcgcgcgc
     1321 atcggaaatg gtgtgggcaa cattcaacag cgcctttgaa ggaatgcatc cgcgattgag
     1381 gcaaacgccg ccaaggcgtt tgtccttctc gatcatgaca accttcttgc ccaaatcagc
     1441 cgcaaaaaaa gcggccgtgt aaccaccggg cccgccgcca accaccacaa tttcagtttt
     1501 aatagattcc atgtttatac gtcggttccg tcattccatc actccgctct tcacatcctg
     1561 ttcgttgaaa ttctccagtg cttgcaaaag atccaccgtg aatctcgccg ctcctccacc
     1621 gtcaatgacg cggtgatcgt aagagagtcc tatcggcagc atcatgcgcg gctcaatttt
     1681 atctttcttc accaccggct tcatcgcgcc tttgccgagc ccaagaatcg ccacttccgg
     1741 cttgttcaca atgggcgtga agtgcgcgcc gccgatgccg ccctggttcg agatggtgaa
     1801 cgtc
//
LOCUS       DASZMM010000356         7314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5089231, whole genome shotgun sequence.
ACCESSION   DASZMM010000356 DASZMM010000000
VERSION     DASZMM010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5089231"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VG754_08610"
     CDS             complement(<1..554)
                     /locus_tag="VG754_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF19316.1"
                     /translation="MLSYIIKRLLHLIPILLGVSLLTFLLMSLTPGDYFTQLSQNPQI
                     SPETLAQLRAAQHLDKPWFIRYGYWLGNALRGDLGYSLAYKTSATQLIMSRLWNTFLL
                     SLGATILAWSVAVPLGIWAAVNKDSWVDRLCSMIAFVGLSVPDVLLALLALMFAAKSG
                     LFPVGGAQSSLYDLMTPREQIWDRL"
     gene            complement(657..887)
                     /locus_tag="VG754_08615"
     CDS             complement(657..887)
                     /locus_tag="VG754_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19317.1"
                     /translation="MTKGAHPRTTPERGVGDPQQCRKEIGAWISLACPVRRALLRLIE
                     PRSVSSGECGEQLPGQSFPASPAYRGQVRGLR"
     gene            1032..2222
                     /locus_tag="VG754_08620"
     CDS             1032..2222
                     /locus_tag="VG754_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19318.1"
                     /translation="MSPRPLNIGLIGGGKGAFIVQPHQRAIHFDGTRRVVAAALFPDP
                     KIAMEEAANWPYPIKGYPSYDEMINVQKSLPKEERLDYVLIVTPNHVHFDPATKALKA
                     GIPVFCEKPLCLNLKEATEMVKIARECKVPFALAHTYLGHWTARFARYIVHSGLLGEV
                     RWVDGSYTQGWLATKLEATGQMQASWRTNPKLAGGAGCGGDIGTHVLMQIRYITGLEI
                     SEVAAHLETFVEGRANDDHFIVYCNLNNGGKALVRCTQIAAGMKNDLGLMISGTKGTL
                     RWRSEDPECVIINLMNQPDRIYWRGGVTPGDGFIPADIPADLMAEPTVPSGHPEAFHD
                     AFARLHRCFEADVRKWQAGGAFAPDGSKYATVEDGWMGIAFIEACVESGSKKGAWTSM
                     PKKI"
     gene            complement(2235..3215)
                     /locus_tag="VG754_08625"
     CDS             complement(2235..3215)
                     /locus_tag="VG754_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HWF19319.1"
                     /translation="MIGASAGGVEATRKLLSELPDDLAAAVLIVIHTGKGPNQLSSVF
                     QRVTRLKLMSPTRDRHAIKNGSVYLAPPNCHMLVNDGHICLSYGPRENLHRPAIDPLF
                     RSAANARGRNVIGVVLSGNLDDGAAGLLAIKECGGITLVQTPEDALFPGMPRNALERV
                     EPDHVVPLAKMGPLLARLTQKPPPRSLKTIPTRVKIESDIADLQMSRNKIDCLGKPSQ
                     YACPDCHGVLWEMKEGGQLRFRCRVGHAYSLESLHEAMTEGSENALWIALRALEEKVS
                     LLQRLAEDSRKRQGMSSARHFERRSKELLPATHAIRALLVKISEKESELK"
     gene            3579..6629
                     /locus_tag="VG754_08630"
     CDS             3579..6629
                     /locus_tag="VG754_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HWF19320.1"
                     /translation="MSNKQVSKSRAKKSSPAVTKPARRAAKAPPAPLEEAVQDTASER
                     KPADFLIVGIGASAGGYEAFLQFLGSLPKDTGMAFTLIQHLDPNHESKLVELLARSTT
                     LPVIEARNGMRVEPNRIYVMPPKVDMRIRDGNLVFQPRTGRTLHMPVDVFFRSLAEHQ
                     QNRAIGIVLSGTGTDGTLGLQAINGEGGITFAQDEKSSKFFGMPGSAINAGVVDVVLP
                     PAAMGRELERIARHPYLGRDLKSKHAARTAAETAENEKIFQEKDGDLNALFALLRART
                     NVDFSLYKHNTLKRRIMRRMLLHKVDSLPNYLARLRQDVAEVDALFNDLLINVTEFFR
                     DAPVFQALKRRIFPRLLRNRPTDATVRIWICGCSTGEEAYSMAISLVEFFETARVHPN
                     VQIFATDISEGSLDKARSGIYPENISLDVSAERLRRFFVKADGGYQVNKSIRDMCVFA
                     RQNVIVDPPFSNLDLISCRNMLIYLGPALQKKIIPAFHYALKPEGILLLGTSEAISAA
                     ADLFTLNDKKFKIYVKKTAAYRPGFPMVYQKDAVQHETLPEPHPLKPENKAPDLQQYV
                     DRMILAKWCPAAVVINSAMDVLLFRGRTGTYLEHAPGNASLNLLKMARDNLMLDLRTA
                     ISKAIKHDIPVRQDNALLRVDGEIRPVSIEAVPFHLSPSSERFYLVLFFENQPGHGAP
                     TPSPSASTTAKPRRESERRIAQLQAELASTKESLQAIIEDQEAANEEFKSANEEIQSS
                     NEELQSTNEELETAKEELQSTNEELTTLNEELQTRNTELTQLNNDLTNLLSSVNIAII
                     MLGEDLAIRRFTPMAKRIFNLIPSDVGRRLTDLNRTINVPDLDEYIHQVLDNLATVDR
                     EVQDREGRWYSLRIRPYRTGDNKIDGAVLMLVDINEHKRAIELFMSAVKQPMVALQTD
                     LRVKSANNAFYEMFHVAPDETEGRYIYDLGNGQWLQPGLRTQLEEIVAKQQPVDDFVV
                     DTEFPKIGKRKMCLDARIFAEDHRNPRLVLLSIRDVSAKAGEVE"
     gene            7116..>7314
                     /locus_tag="VG754_08635"
     CDS             7116..>7314
                     /locus_tag="VG754_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19321.1"
                     /translation="MPPAAESGDGLKERDRLIGELELQLTRLKLEEQEFLRAKRKAED
                     RAAYYADLFKAAPVGFLVFNFS"
BASE COUNT         1651 a         2112 c         2056 g         1495 t
ORIGIN      
        1 agccggtccc aaatctgttc tctcggcgtc atcaaatcgt aaagcgaact ttgcgcgccg
       61 ccgaccggga acaggccgct tttggcggcg aacatcaacg ccagcaaagc cagcaggaca
      121 tccggcacgg acaatccgac gaatgcgatc atcgaacaaa gcctgtcaac ccacgaatct
      181 ttgttcaccg cggcccaaat gcccaacggc accgcgacgg accacgcaag gattgttgcg
      241 ccgagcgaga gcaggaaggt gttccacaac cggctcatga tgagttgggt ggcggaagtt
      301 ttgtaggcga gcgaatagcc aaggtcgccg cgcaacgcgt ttccgagcca gtagccgtag
      361 cggatgaacc agggcttgtc cagatgctgg gccgcacgga gttgggcgag cgtttccggc
      421 gagatctgcg ggttttggga aagttgcgtg aaataatctc ccggcgtaag ggacatcagg
      481 agaaacgtca gcagagaaac acccagcaaa attgggatca aatgcagcag tcgtttgatg
      541 atgtaagaaa gcattacccg ttaaacaaat ccaaaatgcg aaatggccgc gttgaaaacc
      601 caaagcctta tcacgaggtg tacaatagat tcatttgcca tatgccgcaa cctttgtcag
      661 cgcaagccgc gcacttgccc ccgatacgct ggcgatgccg ggaaggactg gccgggcaat
      721 tgctcgccgc attcaccgga cgaaacggag cgcggctcga tgagccgcag caaggcgcga
      781 cgaacaggac acgccagact aatccatgcg cctatctcct tccgacattg ctgcgggtca
      841 ccgaccccgc gctccggggt agtgcgcgga tgcgccccct ttgtcatccg tctccacaaa
      901 agtcttgtga accggaatgt tgatgcgtta cggtattttc gaccaccggc tccagccaga
      961 tgccacccag gctcgcttta aagccggcta aacagaatct cgaaccacca gatcaacaaa
     1021 aggaatcaga tatgtccccg agaccattga acatcggcct gattggcggc ggcaaaggcg
     1081 ccttcatcgt tcaacctcac caaagagcca ttcattttga cggcacgcgc cgcgtcgtcg
     1141 cagccgcgtt gtttcccgat ccgaagatcg cgatggagga agccgcgaac tggccgtatc
     1201 ccatcaaggg ctatccttcc tacgacgaga tgatcaacgt gcagaaatcg ctgcccaagg
     1261 aagagcgact tgattacgtc ctcatcgtca cgcccaacca cgtgcatttc gatccggcaa
     1321 cgaaagcgct caaggccggc atcccggtgt tttgcgaaaa gccgctctgc ctgaatctca
     1381 aggaagcgac cgagatggtg aagatcgcgc gcgaatgcaa agtgccattc gcgctggcgc
     1441 atacttatct gggacattgg acggcgcggt ttgcgcgtta catcgttcac agcggtttgc
     1501 tgggagaagt tcgctgggtg gatggttctt acacgcaagg ctggcttgcg accaaactcg
     1561 aagcgaccgg gcaaatgcag gcgtcatggc gcacgaaccc gaaactcgcc ggcggtgcgg
     1621 gttgtggcgg ggacatcggc acgcacgtct tgatgcaaat ccgttacatc accggcttgg
     1681 aaatcagcga agtggcggcg catcttgaaa cttttgtgga gggtcgcgcg aacgatgacc
     1741 atttcatcgt ctattgcaat ttaaacaacg gcggcaaggc attggtgcgt tgcacacaaa
     1801 tcgcggcggg catgaaaaat gatctcggcc tgatgatttc cggcacgaaa ggcacgttgc
     1861 gctggcgttc ggaagacccg gagtgcgtca tcatcaacct gatgaaccag ccggaccgga
     1921 tttactggcg cggcggagtc acgcccggcg atggtttcat cccggctgac attccggccg
     1981 acctgatggc cgaaccgacg gttcccagcg gccatcccga ggcgtttcac gatgctttcg
     2041 cgcggctcca tcgctgtttc gaagccgacg ttcgcaaatg gcaagcgggt ggcgcgtttg
     2101 ctcccgacgg ctcgaagtac gccacggttg aagacggctg gatgggcatt gccttcatcg
     2161 aggcgtgcgt tgaaagcggc agcaaaaaag gcgcatggac ttcgatgcca aaaaagattt
     2221 gaagcgccag cgcgttattt caattcgctt tccttttcgg aaattttcac gagaagggcg
     2281 cggatggcgt gggtcgcggg cagcaactct ttggagcgtc gctcgaaatg gcgcgcgctg
     2341 gacatgccct ggcgtttgcg ggaatcctcg gcgagccgtt gcaaaagtga aaccttctcc
     2401 tccaacgcgc gcagcgcaat ccagagcgcg ttttccgaac cttcggtcat tgcttcgtgc
     2461 aggctctcca aggaataggc atggccgacc cggcaacgga aacggagttg tccgccttct
     2521 ttcatctccc acaaaactcc atggcaatcg ggacaggcat actgggatgg ttttccgaga
     2581 cagtctattt tgttgcgtga catctgcaaa tcggcgatgt ccgactcgat ctttacgcgg
     2641 gtgggaatgg ttttcaagga gcgcggaggt ggtttttgcg tgaggcgcgc cagcaacggt
     2701 cccattttgg ccagcggcac gacatggtcc ggctccacgc gttccaaggc attgcgcggc
     2761 atgccgggga acagggcgtc ttccggcgtt tgcacgagag tgatgccgcc gcattctttt
     2821 attgcgagca accccgccgc gccatcgtcg agatttcccg acaggacgac tccgatgacg
     2881 tttcggcccc gggcatttgc cgcggaacgg aacaaaggat caatcgcggg acggtggaga
     2941 ttttcgcggg gtccgtatga aaggcagatg tgcccgtcgt tcaccagcat gtggcaattg
     3001 ggcggcgcaa gataaacgga tccgttttta atggcatggc gatcacgagt gggactcatc
     3061 agcttgagcc gggtgacgcg ctgaaagaca ctggacaact ggtttgggcc tttgccggtg
     3121 tggatcacga tcagcaccgc agccgcaagg tcgtcgggca gttcggataa taatttcctt
     3181 gtggcttcaa cccctcccgc cgaggcgccg atcacaataa ggcgcgaccc atcccgttgt
     3241 tgtgctgatg tttcagagtt gattgccatt ctctcccggc tgccagccgg tgccattata
     3301 agttagtaca aattaagcgc gacagcttgg atgtgccaga gcctttcatt gccattttct
     3361 aacatttcat ttcaccacgt atttgccatg gggtaagcgc ctaatattga gattgagaat
     3421 gcgacggttc aagattagct taatggaaaa tgaagctatg ggtttgactg agttgtggga
     3481 tgcctccttt tccagtgctc catcgcccca tccgcttttt gattcgcatt tcaaacagcc
     3541 gcgaaatcgg attaggttcg aatagagaat ccagcatcat gagcaacaaa caagtttcca
     3601 aatcgcgggc caaaaaatct tcgccagccg tcacaaaacc cgcgcgccgc gccgccaaag
     3661 cgccgcccgc gcccctcgaa gaagccgtgc aggacacggc atcggagcgg aaacccgccg
     3721 attttttgat cgtgggcatc ggcgcatcgg ccggcggtta cgaggctttt ctgcaatttc
     3781 tcggcagcct gcccaaggac acgggaatgg cgttcacact catccagcac ctcgatccaa
     3841 atcatgaaag caagctggtc gaactgctcg cgcgctcaac caccctgccg gtcatcgagg
     3901 cccgcaatgg catgcgcgtg gaacccaatc gcatttatgt catgcctccc aaggtggaca
     3961 tgcgcatcag ggacggcaat ctcgtgttcc aaccccgcac ggggcggact ttgcacatgc
     4021 cggtggatgt cttcttccgc tcgcttgccg agcatcaaca aaaccgcgcc atcggaatcg
     4081 tcctctccgg cacgggcacg gatggcacgc tcggccttca ggccatcaat ggcgaggggg
     4141 gcatcacctt cgcgcaggac gaaaaatcat cgaaattttt cgggatgccc ggcagcgcca
     4201 tcaatgccgg cgtggtggat gtcgtccttc cgcccgccgc gatgggccgc gaactggagc
     4261 gcatcgcccg ccatccctat ctcggccggg atttgaaatc gaaacacgcc gcgagaacgg
     4321 cggcggaaac ggcggagaac gaaaaaatat tccaggaaaa agacggcgac ttgaacgcat
     4381 tgtttgcgct gctgcgcgcg cggaccaatg tggatttttc cctctacaag cacaacacgc
     4441 tcaagcggcg catcatgcgc cggatgctgc tgcacaaggt cgattcgctg cccaattatt
     4501 tggcccgtct gcggcaggac gtggcagagg tggacgcgct gttcaacgat ctgctcatca
     4561 acgtcaccga attcttccgc gacgccccgg tctttcaagc gctcaagcgc cgcattttcc
     4621 cgcgcctgct ccggaaccga ccgaccgacg caacggtccg catctggatc tgcggctgtt
     4681 cgaccggcga ggaggcttat tcgatggcca tcagcctggt ggaatttttt gaaaccgctc
     4741 gcgtccatcc caacgtgcag atcttcgcca cggacatcag cgaaggcagc ctggacaagg
     4801 cgcgttcggg gatttacccg gaaaatattt cgctcgatgt atcagccgag cggttgcggc
     4861 gttttttcgt gaaggccgac ggaggctacc aggtcaacaa gtcgatccgc gacatgtgcg
     4921 tctttgcccg ccagaatgtc atcgtggacc cgcctttctc caacctggac ctgatcagtt
     4981 gccggaacat gctgatttat cttggcccgg cattgcagaa aaaaatcatc cccgcgtttc
     5041 attatgcctt gaagccggag ggcattctgt tgctcggcac ctcggaagcc atcagcgcgg
     5101 cggcggacct gttcacgctg aatgacaaga aattcaagat ttatgtgaag aaaaccgccg
     5161 cctatcgtcc cgggttcccg atggtctatc aaaaggacgc cgtgcaacac gaaacattgc
     5221 ccgaaccaca cccgttgaaa ccggaaaaca aggcgccgga tttgcagcag tacgtggaca
     5281 ggatgatcct tgccaaatgg tgtcctgccg ccgtggtcat caactcggcg atggatgtct
     5341 tgttgttccg cgggcgcacg gggacgtatc ttgagcacgc ccccggcaat gcgagcctga
     5401 acctgctcaa aatggcccgc gataatctca tgctcgacct ccgcaccgcc atttccaagg
     5461 ccatcaagca tgatattccg gtccgccagg acaacgcgct gctccgggtc gacggcgaaa
     5521 tacggcccgt cagcattgaa gccgtcccat tccatctctc cccttcaagc gagcgatttt
     5581 atcttgtctt gttctttgaa aaccagcccg gacatggagc gcccacgccg agcccttccg
     5641 cctccacgac cgccaaaccc cggcgcgaaa gtgagcgccg catcgcgcag ctccaggctg
     5701 aacttgcctc caccaaggaa tcgctccagg ccatcatcga ggaccaagaa gccgccaacg
     5761 aggaattcaa atccgccaac gaggaaatcc agtccagcaa cgaggaattg cagagcacca
     5821 acgaggaact tgaaaccgca aaggaggaac tccagtccac caatgaagag ttgaccacgc
     5881 tcaatgagga attgcagact cgcaacaccg agttgaccca gctcaacaat gacctcacca
     5941 acctcctttc cagcgtcaat atcgccatca tcatgttggg cgaggacctt gccatccggc
     6001 gcttcacccc gatggccaaa cgcattttca acctgattcc ttcggatgtg ggccgccggc
     6061 tcactgattt gaaccgcacc atcaacgtgc ccgatttgga tgaatacatc catcaagtcc
     6121 tcgacaatct cgccaccgtg gaccgcgaag tgcaggaccg cgagggacgc tggtattcgc
     6181 ttcgcatccg cccataccgg accggcgaca acaaaattga cggcgcggtg ctgatgctgg
     6241 tggacatcaa cgagcacaag cgggcgattg aattgttcat gtcggcggtc aaacagccga
     6301 tggtggcgtt gcaaacagac ttgcgcgtga agagcgccaa taacgcgttc tacgagatgt
     6361 ttcacgtcgc gcctgatgag acggagggtc gttacattta tgatctgggc aacggccagt
     6421 ggctgcagcc cgggttgcgg acgcagcttg aagaaattgt ggccaagcag cagcccgtgg
     6481 atgattttgt ggtcgatact gagttcccca aaattggaaa gcgcaaaatg tgccttgatg
     6541 cgcgcatctt cgccgaggac caccgcaatc cgcgcctcgt cctgctctcc atccgcgacg
     6601 tgtcggcgaa ggccggagaa gtggagtgat ggatcataga gaactggagt aatggagcaa
     6661 tggggggatg tcataaatag agagcggagc gcgactgtgt ccgcaggacc agtcgcagca
     6721 gcttggaata caggtatttc gcgcgttgct gcggctggcg cttcgcgcac agccgcgctc
     6781 cactattatg agatgtgttc cagggtcacg tggaacgcca gagtctttcc accactccat
     6841 taccccattc cctttctctc ccttgccgca ttctgcaaca atgggttgtt acttggttcg
     6901 aacaggtgct tattccccac gatgacgtgt tatgaaattt gctcgcatta aaagcattga
     6961 cggttcatac ttatcaagga agtcgtgaag ggcgttccaa gagggttaga tgtcgtgcct
     7021 cctgttgaag ctggacgcct ttgtcccgac cgcccgagat catgaaaatg gttcgcaaac
     7081 gacatgtgct attaggcaaa cgctcctttc ccaatatgcc gcccgcagca gagtcagggg
     7141 acggactcaa agagcgcgac aggctgattg gggaactgga gttgcaattg acccggctca
     7201 aattggagga gcaggagttt ttgcgcgcga agcgcaaggc ggaggaccgt gccgcatatt
     7261 atgccgacct tttcaaggcc gcgccagtcg gttttctcgt gttcaatttt tcag
//
LOCUS       DASZMM010000357        10858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2617896, whole genome shotgun sequence.
ACCESSION   DASZMM010000357 DASZMM010000000
VERSION     DASZMM010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2617896"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1472)
                     /locus_tag="VG754_08640"
     CDS             complement(87..1472)
                     /locus_tag="VG754_08640"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HWF19322.1"
                     /translation="MKSDKSDKSTLKKAVKAVTKTIRESSAAVAAQIKKTKSAATRKT
                     VVAKAEPAVPEEPPIVAKRIPKTTASKIKRKVPAKPAAPEVPAILLEGDRPTSPVPGG
                     PGQRYALGPTPPPEHLSGTETAELPESYGTKRLFLTARDPHWLYARWDLTRQQQNQYN
                     RLSTDRHLVLRIFLNQAAGDPLAQVHVHPESTHWFCHVPHAGVKYVAQLGYYETGGNW
                     VGISTSGATLTPPDAMSEDFSAEFATIPVEVPFAQLMELARVAVRENVPLAEVLQQLR
                     ASGYNIPDARPDGRWTPEREQALASIITMDRVRRVWMGSVEITELIRRQLAQEISSMS
                     VAQFSQPGSEFGISSISSISSPFGGLERKKGFWFNVNAELIIYGATEPDAQVTIGGRT
                     IKLRSDGSFSYRFALPDGDYELPAVAVSSDQTDARAAELSFKRATEYHGDVGAHPQDP
                     QLKLPLAASVA"
     gene            2448..2855
                     /locus_tag="VG754_08645"
     CDS             2448..2855
                     /locus_tag="VG754_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19323.1"
                     /translation="MNSKTPANSAAQNAGALIYVVDDEPMLLELAAVILEPQGYNVQT
                     FRDPENAFKAFTTAQPRPSLLITDYAMHSMNGMQLIEKFRALEPRQKIILVSGTVGED
                     IFQEAPARPDRFLAKPYQAGEFTALVKELLTTQ"
     gene            3080..3658
                     /locus_tag="VG754_08650"
     CDS             3080..3658
                     /locus_tag="VG754_08650"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWF19324.1"
                     /translation="MQILAKLNATTLFIQELVNRPREIGAILPSSRNLAHGMARWLPS
                     HSNAYALELGPGTGSVSEALLESGLREDRLIAIEQSPKMADLLRSRFPRATIITGDAF
                     QLDELLKQHPRLAANIGMVFSSLPLLNFEAHIADMLAKKIRSLLPIGGRLVQYSYHLG
                     SRQPKAAAHFRCVASNLIWLNLPPARVSVYQK"
     gene            complement(3683..6025)
                     /locus_tag="VG754_08655"
     CDS             complement(3683..6025)
                     /locus_tag="VG754_08655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWF19325.1"
                     /translation="MEIAGENCKLCEQPVLSGADAAACDDCHSVFHQTCFENVQHICP
                     KCGRINNPAEISDAPVEHPFLPLPEGEFQRLKPSGLYRFSLVVVSLAMILLPVIYVGM
                     IGLLIYSVIAYLVRYAGLMNGLRYGLIGWLMVLLFYIGPMLIGTIMTFFLVKPFFAPR
                     PPQEKPFSLNHADAPQLFALVGWICRSLEAPIPSRIDVDCKVNAGAGFRSGWRSLFGN
                     DIIMVIGLPLVAGMDLAQFAGIIAHEYGHFSQGTAMRASYLINRINAWFFRLVYERDQ
                     WDLALEMASEQEHQSLFTALLLYMARFAVWLTRRLLWVLMATGAALSCFMSRQMEFDA
                     DRYETRLSGSEGFPATTRRLRQLNLGSDAAIKQIRDKWSKERKLFDQIPDFMVSRADE
                     ITAEAQEKLHASLSRRRTRLFDMHPSDDERVQHALAAREPGIFHSTMPAACLFADFSE
                     LSRRVTLNYYQSVFGRQIPAERLISTQQLKNSAEHDYEADRAQIHRYFLGIATELRPV
                     LITENKSLLVRRPDDVLAELKDCRRAMEESFASAEPAFAEFIDSEAKLLQAEQAAQLL
                     QAGFQFEPADFGLTDNETENAQAEACAALEAADARLTSFESAAKTRLTNSIQLLRMPQ
                     FASQIPDATQLQDEAKAMIWVLSRFEEIFVPVLELRKLCAMLDLLLIYRKNQPAADNV
                     APVLESLCMEIQERVNLLQQKTAQIRYPFHHATEQVMVSEYARNKNYHSDPVGLALRE
                     GQSHVEKLLDLYVRLLANLVLIGEEVERCVAPCCGAEENG"
     gene            6230..7408
                     /locus_tag="VG754_08660"
     CDS             6230..7408
                     /locus_tag="VG754_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HWF19326.1"
                     /translation="MQKHPEEQTNKLTKTSLVAHLARVPDPRINRNKDHDLVDILVIA
                     VCALLCAAEAFNDMEDFGKAKRDWFKTFLKLRGGIPSHDTFNRVFAALKPEHFLECFL
                     GWTQSVREAVAQEVVALDGKALRRALNGDQSVKYVVSAWAEGNGLVLGQLKVADKSNE
                     ITAVPELLRVLELSGCIVTMDAMGCQKKIAKEIVEADAHYVLALKGNQETVHEEVKTF
                     LDQTLVEQQTARAPGVQPSAAAAGLVFLETVDKDHGRFEIRRYYQSTELDWFADLRKW
                     EGLKSVGMVESIRELKGKTTIERRYFLSSLPLGVETFARAVRSHWGVENKLHWVLDVC
                     LREDQSRARAGYAAENLATLRRLALNMLKREKTKKRGIRGKQLNASWDHPYLLRLLGI
                     "
     gene            7493..8686
                     /locus_tag="VG754_08665"
     CDS             7493..8686
                     /locus_tag="VG754_08665"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWF19327.1"
                     /translation="MSKIQRSIQVGFELRVFFTRNVFGPANPLLRDVLAGAETQNDRT
                     RRVLVVMDEALAQTQPTLAGQIEAYFANTPRLDLVCPPMIVEGGERTKNSYFHVSEIH
                     SHIDRFHIDRHSYLLAVGGGALLDMAGLAAATAHRGVRHVRLPTTTLSQADSGVGVKN
                     GINAFGKKNFIGTFAPPFAVVNDFEMLASLSERDKRAGYVEAVKVALIRNQEFFDAIE
                     ADADKLASFELASMQSLIQRCAELHLNHIATSGDPFEFGSARPLDFGHWAAHKLEQLS
                     EYKIRHGEAVAVGIALDVIYSRKMGFLDEASAERILRLLEKLGFELYANELLHTDSDH
                     SLMVLNGLEEFREHLGGELAITLLKGIGDGFEVHEMQLPKVIEAIYELRERQATRAKI
                     YHAFG"
     gene            complement(8950..9159)
                     /locus_tag="VG754_08670"
     CDS             complement(8950..9159)
                     /locus_tag="VG754_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19328.1"
                     /translation="MKLFYTGPVVNTEMLVVMLEKHGIAATQEFVEAGAVDDEDLNRP
                     ARVFVPEADYERAHRLFYAEREDEL"
     gene            9285..10100
                     /locus_tag="VG754_08675"
     CDS             9285..10100
                     /locus_tag="VG754_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF19329.1"
                     /translation="MYLEYYGLKEPPFTITPNPRFLFFSSKHREAFNHLLYGIRERKG
                     FVQLTGEVGAGKTTLCRALLEQLGDNFSTALIVNPILDTNEMVKLVAMEFGLEVRGMD
                     RLEIVAVINDFLLRQMAENRETVLIIDEAQDLTNELLEQVRLLSNLETDDRKLLQIVL
                     MGQPELRDRLNAHSLRQLRQRITVRYHLNPLRRGEVAQYVQHRLQVSGAQGAPYFTQP
                     ALWRVYNYSKGIPRLVNAVCDKALLAGFVEQRDRIDFRLVGRALRELEGNIAA"
     gene            10097..10693
                     /locus_tag="VG754_08680"
     CDS             10097..10693
                     /locus_tag="VG754_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19330.1"
                     /translation="MSLLNDALKKAKNTQPNASATADGPILRPDFAQGRVRAGNDFLL
                     PALIVIILLLAALLLWQWFRAETGEIKVRARTIAPSEIVPTGPVHEAVAASVPQPALA
                     PEKISATNASAATVTPATVVTNAPVVVPVPAAPAPPPPVTYKLQSIFYRAKNPSTVIN
                     GKTLFIGDRVAGAHVTAIGQDSVTILTEKGETKVMELP"
BASE COUNT         2417 a         2971 c         3029 g         2441 t
ORIGIN      
        1 aagaccgcgc agattccgcc ccggatgtaa tggaacattg agagcagctt tagatgttcg
       61 gcatcgcgct ccattgctgt ctgcgcttat gccacgctgg ccgcaagcgg gagtttgagt
      121 tgcggatctt gcgggtgcgc gccgacgtcg ccgtgatatt cggtggcgcg tttgaaactc
      181 aactcagcgg cgcgggcgtc ggtctggtcg gaagaaaccg cgacggcggg caattcataa
      241 tcaccatccg gcaatgcgaa gcgatagctg aaactcccat ccgaacggag cttgatcgtg
      301 cgcccgccga tggtcacctg cgcgtccggt tccgttgcgc cgtagatgat caattccgca
      361 ttgacgttga accaaaaacc ttttttgcgc tccaatccgc cgaacgggct ggagatgctg
      421 ctgatgctcg aaatgccgaa ctccgagccg ggctgcgaga attgcgccac gctcatcgaa
      481 gaaatttcct gggccaattg ccgccggata agttcagtga tttccactga gcccatccaa
      541 acgcgccgca cgcggtccat ggtgatgatg gaagcgaggg cttgttcgcg ttcgggtgtc
      601 catcttccgt cgggccttgc atcgggaatg ttgtagccac tcgcgcgcaa ttgctggagc
      661 acctcggcca gcggaacatt ttcgcggacg gcaacacgcg ccagttccat gagttgcgcg
      721 aacggcactt caacgggaat cgtggcgaac tcggcggaga aatcttcgga catcgcgtca
      781 ggcggcgtga gcgtcgcgcc ggaagtggag atgccgaccc aattgccgcc agtctcgtaa
      841 taaccgagtt gcgcgacata tttcacgccc gcgtgcggca catggcagaa ccaatgggtg
      901 gactcgggat gaacatgcac ctgcgccagc gggtcaccag cggcctggtt caggaagatg
      961 cgcaaaacca aatggcggtc cgtggaaaga cgattgtatt gattttgctg ctgccgcgtg
     1021 aggtcccaac gcgcataaag ccaatgcgga tcgcgcgccg tgaggaacag ccgtttggtg
     1081 ccgtaagatt ccggcagttc cgccgtttcc gtgccgctca aatgttccgg cggcggtgtc
     1141 ggtcccaagg cataacgctg ccccggtccg cccgggactg gcgacgtcgg acggtcgccc
     1201 tcgagcaaga ttgccggcac ttccggcgcg gcaggcttgg cagggacttt ccgtttgatt
     1261 ttggatgccg tggttttggg aatgcgcttg gcgacaatgg gcggctcttc tggaaccgct
     1321 ggttcggctt tggcgacaac tgttttgcgc gtcgcggcag atttggtttt cttgatttgg
     1381 gcggcaacgg cggcgctgct ttcgcggatg gttttggtga ccgctttgac cgccttcttc
     1441 aaggttgatt tgtcggactt atcggatttc atacgagttc cggggcaagg ctggcaagtt
     1501 gatgcctgtg accaacggca ttctacggcg ggtaactcgc ctgcgcaacg ggaatctgga
     1561 gcctggttca tgaccaaaat attcgatgct ggtaatgatt accctgcaac gtctcgccaa
     1621 gcgacattaa gcgacacaac ccggatcagg cgaggttaga ggatcgagga tggcgacgga
     1681 cgccaacttt aaaccttaaa ctcgaaacct tgaactttcc gcagggaggg gggtgccgtt
     1741 ttgtggcggg tttaggtgaa gctatgcggg tttaagcggg attacaacca cttacaactt
     1801 tccgagccgg tagggatgac ctgctggtcg tccgcaccga cgcgcggcag cgcgtcccta
     1861 cctttctgga tccaagcaaa aaaatatttt attttctatc ccggtttatc cgagaatatc
     1921 tgagttgatg tgagttgatc tgaggcaatt tgaaacaaac cagagtcaaa ggtgaagttt
     1981 cagccccaaa ctgaccagat gcttggttgc ggatgctgga tatggcctga agcctcagtc
     2041 actcagcctt caaccatttt aggatgtcaa agaacatgcc cgcctcccaa cggcaacgag
     2101 agtatcacag tccattttgc aggcaagaaa tttcggcaaa aacttgtcgc acgaagtccg
     2161 cgaaagaaac gaagtttgaa cggcgaaacg cattttgccc ggcgagacca tcccaacggt
     2221 ttaacgcggt atgtcatttc ccctgcgcgc cccatgccaa gagaccgcaa ctacggtgct
     2281 gccgcggctg tttcggcaaa ccctttacaa aatgcagcct tgaaagtatg ggtaattttg
     2341 agcggttttg ggagggataa tccgcagaaa gagcgcttgc caagcgctgg gaattgattc
     2401 ttaatttgta gagtctccca gagggtgtcg agaggaagac caagactatg aacagcaaaa
     2461 cgccagccaa ttcagcggca caaaatgccg gggccctgat ttatgtcgtg gatgacgaac
     2521 cgatgttgct ggaattggcc gcagtcattc tcgaaccgca gggctataac gtccaaactt
     2581 ttcgcgatcc ggaaaatgcg ttcaaagcat tcaccacggc gcaaccccgc ccgtccttgt
     2641 tgatcaccga ttacgcgatg cattccatga atgggatgca gctcatcgaa aaattccgcg
     2701 ccttggaacc ccgccaaaaa atcatcctgg tcagcggcac agtcggtgag gacatttttc
     2761 aggaagcgcc tgcgcgtccc gaccgttttc tcgccaagcc gtatcaagcg ggcgaattca
     2821 ctgcgctcgt caaggagttg ctcacaacgc aatgactccg tgttgcaata tgcgacgcac
     2881 ctgcgaagca attcccccgc gtttttccgg ctcaactttt ttctcgcgcc ccggaggatg
     2941 ttcgactatt gtttgcgcga atttgagaca tggggttcct taggtaatcg aacaaataat
     3001 cgaattgaac gtcaaatgcg cggacggtgg gtgggtacaa tcatcgtttc tgggaatata
     3061 aaagccttaa acaactaaaa tgcaaattct tgccaagctc aatgccacga cgctcttcat
     3121 tcaggaattg gtcaaccgcc cccgcgaaat cggcgccatt ctgcccagtt cgaggaacct
     3181 tgcgcacggg atggcgcgct ggctgccgtc ccattccaac gcgtacgcat tggaactcgg
     3241 ccccggcaca ggctctgttt ccgaagcgct tttggaaagc ggcctgcgcg aggaccggct
     3301 catcgccatc gaacaatctc cgaaaatggc cgacctcctg cgctcccgtt ttccccgcgc
     3361 gaccatcatc accggcgatg cctttcaatt ggatgaattg ctcaaacaac atccacgttt
     3421 ggctgcgaac atcggcatgg ttttttccag tttgccgttg ctgaattttg aagcgcacat
     3481 tgccgacatg cttgcaaaaa aaatccgctc attgcttccc attggcggcc gacttgtgca
     3541 atacagctat catctcggca gccgtcagcc caaggcggcg gcgcatttcc gttgcgtcgc
     3601 ctccaatctc atctggctca acctcccgcc cgcgcgcgtg agtgtttacc aaaaatgatc
     3661 acgtagctga aaacgtgagt tttcagccgt tctcttccgc gccgcaacag ggcgcaacgc
     3721 aacgctcaac ttcttcgccg atcaaaacaa ggttcgccaa caggcggaca taaagatcca
     3781 gcaatttttc gacatggctt tggccttcgc gcagcgcgag tccaaccggg tctgaatgat
     3841 aatttttgtt gcgcgcgtat tcgctcacca tgacttgttc cgtcgcatga tggaacggat
     3901 aacggatctg cgcggtcttt tgctggagaa gattgacgcg ttcctggatt tccatgcaaa
     3961 ggctttccag cacgggcgcg acgttgtcgg cagcgggttg gtttttgcgg tagatgagca
     4021 gaaggtcgag catggcgcaa agtttgcgga gttccagcac cggcacaaaa atttcctcga
     4081 accgtgaaag cacccagatc atcgccttcg cttcatcttg aagttgagtg gcgtcgggaa
     4141 tctgagaagc gaattgcggc atgcgcaata gttgtattga attcgtcagg cgtgtcttcg
     4201 cggcgctttc gaaagatgtc aatcttgcgt cggctgcttc taaagcggcg cacgcttctg
     4261 cctgggcgtt ttcggtctca ttgtccgtca ggccgaaatc agcaggttca aattgaaatc
     4321 ccgcctgcaa taattgcgcg gcttgttctg cttgcagcag cttcgcttcc gaatcgataa
     4381 attcggcaaa cgcaggttca gccgacgcga aggattcttc cattgcgcgg cggcaatcct
     4441 tgagttcggc cagcacatcg tccggccggc gcacgagcag cgatttgttt tccgtgatga
     4501 ggaccgggcg caattcggtg gcgatgccca aaaaataacg gtgaatctgc gcgcggtccg
     4561 cttcgtaatc atgttccgcg ctgttcttga gctgttgggt ggagatgagg cgctcggcgg
     4621 gaatctgccg gccaaaaacg ctttgataat aattcaacgt gacgcggcgg gagagttcgg
     4681 aaaaatccgc aaacaaacac gcggcgggca tcgtcgagtg aaaaataccc ggttcacggg
     4741 cggcgagggc atgctggacg cgttcatcat ccgagggatg catgtcgaac aggcgcgtcc
     4801 gccggcgtga aagcgacgcg tgcaattttt cctgcgcttc ggcggtgatt tcatccgcgc
     4861 ggcttaccat gaagtcgggg atttgatcaa acagcttgcg ttccttggac catttgtcgc
     4921 ggatttgctt gatggcggcg tccgacccga gattgagctg gcgcagccgg cgcgtggttg
     4981 ccggaaagcc ttcgctgccg ctcaaacgcg tctcataacg gtccgcatcg aattccattt
     5041 gccggctcat gaaacaactc aatgccgctc ccgttgccat gagcacccac aacaatcgtc
     5101 gcgtgagcca gaccgcaaac cgcgccatgt aaagcagcaa cgcggtgaac aggctctggt
     5161 gttcctgctc cgatgccatt tcaagagcga ggtcccattg gtcgcgttcg taaacgagcc
     5221 ggaaaaacca cgcattgatc cggttgatca aatacgacgc gcgcatcgct gtgccttgcg
     5281 agaaatggcc gtactcgtgc gcaatgattc ccgcgaactg cgcaaggtcc atccccgcca
     5341 ccagcggcaa gccgatgacc atgatgatgt cgttgccaaa gagacttcgc cagccgctgc
     5401 gaaagcctgc gccggcgttc accttgcaat ccacgtcaat gcggctggga atcggcgctt
     5461 ccaacgagcg gcaaatccat ccgaccaatg cgaaaagctg cggagcatcg gcatggttga
     5521 gcgagaaggg cttttcttgg ggcggacgcg gcgcaaaaaa aggtttcacg agaaagaagg
     5581 tcattatcgt tccgatcagc atcgggccga tataaaaaag aagaaccatc agccatccta
     5641 tcagtccgta tcgtaaaccg ttcatgagtc cggcgtacct gacaaggtaa gcaatgaccg
     5701 aatagatgag caatccgatc attcccacat aaatcaccgg caacaagatc atcgccagcg
     5761 aaaccaccac aaggctgaag cgatataatc cggagggttt gagacgctgg aattcgcctt
     5821 cgggcaatgg caggaatgga tgttcaacgg gcgcatcgga aatttccgct ggattgttga
     5881 tccggccgca ttttgggcag atatgttgga cgttttcgaa gcaggtttgg tgaaaaacgg
     5941 agtggcaatc gtcgcaggcg gcggcatcgg ccccggaaag gacgggctgt tcgcacagtt
     6001 tgcagttttc cccggcgatt tccattcagt tcaattacta aagacggccg gcttcttcac
     6061 agcagtctcg ggttattatt caaacgcact ctgtgccttt tgtcaccgtc cgtcaaggcg
     6121 gggcagccga ttctccaggg cagacgcata taaattggag agtaaacaaa agccagggtg
     6181 tgacaaattg gcactgtact tttgtgtaca tgtgtttaaa taaggaggga tgcaaaagca
     6241 tcctgaagaa caaactaaca agctgacaaa aaccagtctg gtggcgcatc ttgcccgcgt
     6301 gccggacccg cgcatcaacc gcaacaaaga ccatgatctg gtggacattc tggtgatcgc
     6361 cgtctgtgcg cttttgtgtg cggcggaggc gttcaacgac atggaggatt ttggcaaagc
     6421 caagcgcgac tggttcaaga cgtttctgaa actgcgcggc gggattccct cgcacgatac
     6481 cttcaaccgg gtttttgccg cgctcaaacc ggagcatttt ttggagtgtt tcctgggctg
     6541 gacacaaagt gtgcgggagg cggtggcgca ggaggtcgtc gctttggacg gcaaggcgct
     6601 gcggcgggca ttgaacggcg accagagcgt caaatacgtg gtcagcgcgt gggcggaagg
     6661 caacgggctg gtgctgggac agttgaaggt ggcggacaag agcaacgaaa tcacggcggt
     6721 gccggagttg cttcgcgttc tggagttgag cggctgcatc gtgacgatgg acgccatggg
     6781 ctgtcagaag aagatcgcca aagaaattgt ggaggccgat gcgcattacg tgctggccct
     6841 caagggcaat caggagacgg tgcatgagga ggtgaaaaca tttttagacc agaccctggt
     6901 ggagcagcaa actgcgcgcg caccgggagt ccaaccgtcc gcagcggcgg cgggtctggt
     6961 tttcctggag acggtggaca aggaccacgg acgttttgaa atccgccgct actatcaaag
     7021 caccgaactg gattggtttg cggatttgag aaaatgggag gggctcaaat ccgttggcat
     7081 ggttgaatcc atcagggagt tgaagggcaa aaccaccatc gaacgcagat actttctgtc
     7141 gagcctgccc ctgggagtgg agacctttgc ccgggcagtg cgcagccact ggggcgtgga
     7201 aaacaagctg cactgggtgc tggacgtttg tctccgggaa gatcaaagcc gcgcccgcgc
     7261 cggctacgcg gcggaaaatc tcgccactct ccgccgtctc gctctcaaca tgctcaaacg
     7321 agagaaaaca aaaaagcgtg gcatcagggg aaaacaactc aacgcgagct gggatcatcc
     7381 ctatttactc cgattattgg gaatttaaat gcgtctgccc tggccgattc tcgcattcct
     7441 caaattttaa ataaaactcg atgttatgcg tctaaccatc cagattactg cgatgtccaa
     7501 aattcagcgc tccatacagg tcgggttcga gttgcgggtt tttttcaccc gcaatgtctt
     7561 cggtcccgcc aatccgttgc tgcgcgatgt ccttgccggc gctgaaacac aaaacgaccg
     7621 cacccgccgt gtgctggtgg tgatggatga agcgctcgcc caaacgcaac cgacgctcgc
     7681 cggtcaaatc gaagcttatt tcgcaaacac gccacggctc gatctggttt gcccgccgat
     7741 gattgttgaa ggcggcgagc ggacaaagaa ttcttatttt cacgtctcgg aaatccattc
     7801 gcacatagat cgtttccaca ttgaccgcca ttcctatctg ctggcggtcg gcggcggcgc
     7861 attgctggac atggccggtc tcgcggcggc aacggcgcat cgcggcgtcc gtcacgtgcg
     7921 ccttccgacc accacgctca gccaggcgga ttccggcgtg ggcgtcaaaa atggcatcaa
     7981 tgccttcggc aaaaagaatt tcattggcac gttcgcgccg ccgttcgccg tggtgaatga
     8041 tttcgagatg ctcgcgtcgt tgtctgaacg cgacaagcgc gccggatatg tcgaagccgt
     8101 gaaggtcgcc ttgatccgca accaggaatt ttttgatgcc atcgaagccg atgccgacaa
     8161 gctcgcatcc tttgaacttg cgtcgatgca gagtctcatc caacgctgcg ccgaactgca
     8221 cctgaaccat attgcgacgt cgggagatcc ttttgaattc ggctcggcgc gcccattgga
     8281 ttttggccat tgggcggcgc acaagcttga gcaattgtcc gaatacaaga ttcgccatgg
     8341 cgaagcggtc gccgtcggca tcgcgctcga tgtgatttat tcgcgcaaga tgggttttct
     8401 cgacgaagcc tctgccgaac ggattttgcg tttgctcgaa aaactcggct ttgaacttta
     8461 tgccaacgaa ctgctccaca ccgattccga ccattcgctc atggtgttga acgggttgga
     8521 ggagtttcgc gagcacttgg gcggcgaact tgccatcacg ctgctgaaag gcatcggcga
     8581 tggctttgaa gtccacgaga tgcaattgcc aaaggtcatc gaagccattt acgaacttcg
     8641 ggaacgtcag gccacacgcg ccaagattta tcacgcgttt ggatgagcct tgcttccgga
     8701 tttttaccac cgatgaaaca cggattgaac acggagaaaa tgaatcgcag aaaaacttgg
     8761 ttatttgtgc ttctaatcgt ctccgcgatt ttagcggcgt gtataattca gattcacaag
     8821 gtaattccga atcttctgca ttgaagcact gttttacccg aagctctcct acttacttta
     8881 tccgcaagga atcatttccc tcagtcttgt tccgtgttca atccgtgttt catccgtggc
     8941 tcaacgtcat cacaattcat cctcgcgctc ggcataaaac aatcgatgcg cgcgctcgta
     9001 atccgcttca ggcacgaaca cccgtgcggg acgattcaaa tcctcgtcat ccaccgcacc
     9061 cgcttcgaca aattcctgcg tcgccgcgat gccgtgcttt tccagcatca cgacgagcat
     9121 ttcggtgttc accacagggc cggtgtagaa gagtttcatc gttggttaca acgttacaag
     9181 cttacatgga ttcagggtcg cgggccattt tgtaaatctg gctgtcagaa tgccttaaaa
     9241 atttcttctc tttcctgctt ttcaggttct gttaaagata aggcgtgtac ttggaatatt
     9301 atggcctcaa agagccgcca ttcaccatca cgccaaaccc gcgctttctg tttttcagca
     9361 gcaagcatcg cgaggcgttc aatcatttgc tttacggcat ccgcgagcgc aagggcttcg
     9421 ttcagttgac cggcgaagtc ggcgcgggca agacgacgct ttgccgcgcg ttgctcgaac
     9481 aacttggtga caatttttcc accgcgctca tcgtcaatcc tatcctcgat actaatgaaa
     9541 tggtcaaact cgtggcgatg gaattcggcc ttgaagtccg gggaatggac cgtttggaga
     9601 tcgtcgcggt catcaatgat ttcctgttgc gccagatggc cgagaaccgg gagacggtgc
     9661 tcatcatcga tgaagcccag gacctgacca acgaattgct cgaacaagtg cggctgctgt
     9721 ccaatctgga gacagatgac cgcaagctgc tgcagatcgt cctgatgggc caaccggaat
     9781 tgcgggaccg cctgaacgcc cacagtttgc ggcaattacg ccagcgtatc acggttcgtt
     9841 accatctgaa tcccctgcgg cgcggggaag tggcacagta cgtgcagcat cggttgcagg
     9901 tatccggggc gcaaggcgcg ccttatttta cgcagccggc cctttggcgg gtttataact
     9961 acagcaaagg catcccgcgg ctggtaaacg cagtttgcga caaggcgttg ctggccggtt
    10021 ttgtcgaaca acgcgaccgg atcgatttcc ggctggttgg acgggcgttg cgggagctgg
    10081 aaggaaacat cgcagcatga gtttgctcaa tgatgccttg aaaaaggcga agaacacgca
    10141 gccaaacgct tcggcgacgg ctgacggccc cattttgcgc cctgattttg cccaaggccg
    10201 cgtccgcgcc ggcaacgatt tcctcctccc cgcgctgatt gtcattattt tgctcctggc
    10261 cgcacttctg ctttggcaat ggttccgcgc cgaaacaggc gaaatcaaag tgcgcgccag
    10321 aacgattgct ccgtcggaaa ttgtcccaac cggaccggtt catgaagctg tcgccgcttc
    10381 cgttccgcaa ccggcgctcg ctccagaaaa aatttctgcc acaaacgcat ccgctgctac
    10441 cgtgacgcct gcaaccgtcg tcaccaacgc tcctgttgtt gtcccggtgc ctgccgcgcc
    10501 cgcgccaccg ccgccggtga cgtataaatt gcaaagcatt ttttaccgcg ccaaaaatcc
    10561 ttccaccgtc atcaatggca agacgttgtt catcggcgat cgcgttgccg gggcgcacgt
    10621 gacggccatc ggccaggact ccgtcacaat cctcaccgaa aagggtgaga cgaaggtgat
    10681 ggaattgcct taagaaagct ccaatctcca agcgccaaag aataccttct ggaacgccat
    10741 tgatgcttgg cgcttggtta ttctctggtg cttggatgtt ggaatttggt gctttctgtg
    10801 tttccccttg gcagtgagaa aacgatttga ttagctgtgc gcgcccgatg agaaccgc
//
LOCUS       DASZMM010000358         5955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_960164, whole genome shotgun sequence.
ACCESSION   DASZMM010000358 DASZMM010000000
VERSION     DASZMM010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960164"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VG754_08685"
     CDS             complement(<1..197)
                     /locus_tag="VG754_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HWF19331.1"
                     /translation="MDPALKIDSDRLQRELDELAGISSAPAPVVTRVLFSEADLRARA
                     FVKKRCEEANLVLREDAVGNI"
     gene            complement(263..883)
                     /locus_tag="VG754_08690"
     CDS             complement(263..883)
                     /locus_tag="VG754_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWF19332.1"
                     /translation="MKPPIPKNEKRRLEVLWQYDILDTVPEQVFDDLTELAASICEAP
                     VALITLVDDKRQWFKSRQGISATETTRDVSFCGHAIMQDKLFIVPDATHDARFAHNPL
                     VLGEPQIRFYAGAPLVTPDGHALGTLCVIDKVPRTLRPEQKRAMEILSRHVMTQLELR
                     RRSLELASVREERENLLCDLRKMRVELAQTRRQLDAVRKKPVKVRR"
     gene            complement(893..1639)
                     /gene="allE"
                     /locus_tag="VG754_08695"
     CDS             complement(893..1639)
                     /gene="allE"
                     /locus_tag="VG754_08695"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HWF19333.1"
                     /translation="MQTTTELFGATRNVVKERYALLTPSGFVPSVLPGWDDATCIVLI
                     SPAMGARFCQFLITLGKEGHGRGNTGKTEYFAYVLEGSASVTLDAKRNRMEAGSFAYI
                     PPGKDIHFQGSHAKLLIFQKNFVPLSATEVPKVIIGHQREAKGHPFLGNEDARLQVLL
                     PDNPQFDMAVNIFTYQPGATLPFVETHVMEHGLLMLSGQGVYRLDADHHPVRAGDVIW
                     MAPYCPQWFVAMGKTPASYLYYKDVNRDPM"
     gene            complement(1623..2999)
                     /locus_tag="VG754_08700"
     CDS             complement(1623..2999)
                     /locus_tag="VG754_08700"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HWF19334.1"
                     /translation="MPEVDLILRGGTVVTAENSQTADIGVAEGKIVAIQARLQSSCKE
                     EINAQGLHIFPGIIDSHVHFNEPGRADWEGFETGTRALAAGGATLFFDMPLNAHPPTV
                     DAASFDLKLAAARGKSLVDFAFWGGLVPGNVDKMEELAARGVVGFKAFMSNSGIEDFA
                     CVDDATLRAGMKEAARLKKMVAVHAESETMTRELTQEKLRAGKTGIRDYLDSRPIAAE
                     LDAILRALDLAGETGCALHVVHVSSGAGVGLIAEARQSGLDVTCETCPHYLTLTEDDM
                     LKLGAVAKCAPPLRPTEEREALWQLVAEGKITTIGSDHSPSPPGMKTGVDFFKVWGGI
                     SGGQHTLPLLLSEGHVNRGIALPLLAGLVSFNVAKRFNLPSRKGEIKAGCDADLALVK
                     LDETSTISAEDLFYRHRQSPYVGRALRGRVRQTLLRGRTIFKDGKITAKLLGELVTGG
                     NHGHANDD"
     gene            complement(2992..4254)
                     /locus_tag="VG754_08705"
     CDS             complement(2992..4254)
                     /locus_tag="VG754_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HWF19335.1"
                     /translation="MNHNELARTVMERIEALARISEEPGQLTRTFCSTAMRQANELVA
                     GWMREAGMDVTQDAIGNVIGRYPARDEHAKSFILGSHLDTVRDAGKFDGPLGVLTAIA
                     CVKHLHEQKIHLPFALEVVGFADEEGVRYQMTYLGSKALVGKLNERDLQRADANGILM
                     GEAIRSFGGNPDQLKSARRDADRLLGYAEVHIEQGPVLEEKKQPVGVVSAIAGQTRIK
                     AIFTGKAGHAGTTPMALRHDALAAAAQFIIAVESFAKGQPGLVATVGQIEAKPGASNV
                     IPGEVTLSVDVRHADNGQRHEACAKLKDAAHQVGEKRGVTVSWEVVHETSSVKCSHEL
                     TALLAKAACEHVKEVVELPSGAGHDAAVMADIAPIAMLFVRCKGGISHHPDESVMTED
                     LAVAIAVMNDFLQLLSNQQSLFKAKSHA"
     gene            complement(4255..4611)
                     /gene="uraH"
                     /locus_tag="VG754_08710"
     CDS             complement(4255..4611)
                     /gene="uraH"
                     /locus_tag="VG754_08710"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HWF19336.1"
                     /translation="MPAKLSTHVLDTANGCPAQGMQIELWSLDDAEPKLLKSARTNED
                     GRTDEPLLAGEELKAGLYELVFHVGDYFDEKTDTRAEFPFLDEVPVRFGIADAKASYH
                     VPLLVSPWSYSTYRGS"
     gene            5021..>5955
                     /locus_tag="VG754_08715"
     CDS             5021..>5955
                     /locus_tag="VG754_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWF19337.1"
                     /translation="MFSQKSKPIIERCERDMNTRLRLKYEPYYHVVDAVDGVNVTVEG
                     RPMVMMSSNEYLSLSRHPKVIAAAKRALDQWGTSPCGSRLANGSRRYHIELEEALAAF
                     LGQESCHVLSAGYLACISSLSSLVQRGDTLIVDPGIHSSLWDGALLSGAKLERFEHEN
                     TASLAKVLGALDARQAKAIVVDGVYSMEGHIASLPKICELAEQHQAALIVDDAHGIGI
                     LGREGRGVCDHLGVTERVDLIVGSFSKSLVGTGGFVAGSKALIEYLRSNSRQIIFSAA
                     ISPAAAAAALAALEVSRSEPEHRERLWANYHHLRA"
BASE COUNT         1239 a         1777 c         1613 g         1326 t
ORIGIN      
        1 aaaatattgc cgacggcatc ttcgcgcaaa acaaggttcg cctcttcaca ccgttttttt
       61 acaaacgccc gcgcgcgcaa gtcggcctcg gaaaacaaaa cgcgcgtgac cactggcgcg
      121 ggcgcggagg aaataccggc caactcatcc agctcacgct ggagacggtc gctgtcaatt
      181 ttaagcgctg gatccaaaat tcaaaaagca gcagaaggcg aaccgtatca agaggccacg
      241 tgccattcga tcttcgtgaa aatcagcggc gaactttgac cggctttttg cggacagcgt
      301 ccaactgcct ccgcgtttgg gcgagttcga cgcgcatttt ccggaggtcg cacaggaggt
      361 tctcccgttc ttcgcggaca ctggccaatt caagcgagcg gcggcgcaat tcaagctgcg
      421 tcatcacatg gcggcttaaa atttccattg cgcgcttctg ttcagggcgc aacgtgcgcg
      481 gcaccttgtc aatcacgcaa agcgtcccga gcgcgtgacc gtccggcgtg acgagcggcg
      541 cgccggcgta aaaacggatt tggggttcgc ccaaaaccaa tggattgtgc gcgaaccgcg
      601 cgtcatgcgt ggcgtcgggg acgatgaaca gcttgtcctg catgatcgcg tgaccgcaaa
      661 atgaaacatc gcgtgtggtt tcagtcgcgc ttatgccttg cctggacttg aaccattggc
      721 gtttatcatc caccagcgtg atcaacgcga ccggcgcttc gcagatgctc gccgcaagtt
      781 cggtcaggtc atcgaagact tgttcgggca cggtatcgag gatgtcgtat tgccagagga
      841 cctcgagccg tcttttttcg tttttgggaa tgggtggctt catgtttcct tttcacatcg
      901 ggtcgcggtt cacgtcctta taatagaggt agctcgcggg cgttttgccc atggcgacaa
      961 accattgcgg acaataaggc gccatccaga tgacatcacc ggcgcgcaca ggatgatgat
     1021 cagcatcaag ccggtaaacg ccctggccgg acaacatcag caggccatgt tccataacgt
     1081 gcgtttccac gaagggcagc gtcgcgccgg gttgataggt gaaaatattg acggccatgt
     1141 cgaattgcgg attgtccggc agcaacactt gcagacgcgc gtcttcgttg ccgagaaatg
     1201 gatggccttt cgcctctcgc tggtggccga tgatgacctt tggcacttca gttgcggaaa
     1261 gcgggacgaa atttttttga aaaatcagca gcttcgcgtg ggaaccttgg aaatgaatgt
     1321 ccttgcccgg cggaatgtac gcgaaactgc ccgcttccat ccggttgcgt ttggcgtcga
     1381 gcgtcacgct ggcgctgcct tccagcacat aggcgaaata ttccgtcttg ccggtgttgc
     1441 cgcggccgtg gccctctttt cccaaggtga tgaggaactg gcaaaatcgc gcgcccattg
     1501 cgggcgaaat caatacgatg caggtcgcgt cgtcccatcc cggcagcacg ctcggcacaa
     1561 accccgatgg cgtcagcagc gcgtaacgct ccttgaccac atttcgtgtc gctccaaaca
     1621 attcagtcgt cgtttgcatg tccgtggttc ccgcccgtca ccaattctcc taatagcttg
     1681 gccgtgattt tgccatcttt aaatattgtg cgcccgcgca gcaatgtttg ccgcactcgc
     1741 ccgcgcaagg cgcgcccaac ataagggctt tgacgatgcc ggtaaaacaa atcttcggcg
     1801 gaaattgtgg acgtttcatc gagcttcacc agcgcaagat cggcatcgca acccgctttt
     1861 atctcgcctt tgcgcgacgg cagattgaaa cgctttgcga cgttgaagga aaccagcccg
     1921 gcaagcaacg gcaaagcaat tccccgattc acgtggccct cgctcaacaa caagggcagc
     1981 gtgtgttgcc cgccggagat gccgccccaa accttgaaaa aatccacgcc ggttttcatg
     2041 ccgggcggcg atggcgaatg gtccgagccg atggtcgtaa tttttccttc tgccacaagc
     2101 tgccataatg cttcgcgctc ttcggttgga cgcaatggcg gagcacattt cgccaccgcg
     2161 ccaagtttca acatgtcatc ttctgtcaac gtgaggtaat gcgggcacgt ttcgcaggtc
     2221 acatccaggc cggattgccg cgcctcggcg atcaaaccga cgccagcgcc gctgctgaca
     2281 tgcaccacgt gcaatgcaca gccggtttcg ccagcaagat cgagcgcgcg caggatggca
     2341 tccagttccg cggcgatggg acgcgaatcg agataatcgc ggatgccggt cttgccggcg
     2401 cgcaattttt cttgcgtcaa ttcgcgggtc atcgtttcgc tctcggcatg gacggcgacc
     2461 atttttttca accgcgcggc ctctttcatc cctgcgcgca gagtggcatc gtcaacacag
     2521 gcgaaatctt caatgccgct gttggacatg aaagccttga aacccaccac gccgcgagcg
     2581 gccaactcct ccattttgtc cacattgccg ggaaccaatc cgccccaaaa cgcaaaatcc
     2641 accaacgact tccctcgcgc tgctgccaat ttcagatcga agcttgccgc atcaacggtt
     2701 ggcggatggg cattaagcgg catgtcgaaa aaaagcgtgg cgcctcccgc tgccaacgcg
     2761 cgtgtgccgg tttcgaagcc ttcccaatcc gcgcggcccg gttcgttgaa atgcacgtgc
     2821 gaatcaatga tgcccgggaa aatgtgcaaa ccttgcgcat tgatctcctc cttgcacgag
     2881 ctttgcaggc gggcttgtat tgcaacaatt tttccttccg ccacgccgat gtcggcagtc
     2941 tgcgaatttt cagcggtgac gactgtcccg ccgcgcaaga tgaggtccac ttcaggcatg
     3001 gctctttgct ttgaaaaggc tttgctgatt cgacaaaagc tggagaaaat cgttcatcac
     3061 cgcgatggca acggccagat cttccgtcat cacggattca tccggatgat ggctgatgcc
     3121 gcctttgcaa cgcacaaaga gcatggcaat cggagcgatg tcggccatca ccgccgcatc
     3181 gtgtcccgcg ccactgggca attccacgac ttccttcaca tgttcgcacg ccgctttggc
     3241 cagcaacgcg gtcaattcat gtgagcattt taccgaggac gtttcatgca ctacttccca
     3301 cgaaaccgtc actccgcgtt tttcaccgac ttgatgcgcc gcgtccttca atttcgcgca
     3361 ggcttcgtgc cgttggccat tgtcggcatg acgcacatcc acgctcaacg tgacttcgcc
     3421 cggaatcaca ttgcttgcgc caggctttgc ctcgatttgc ccaacggtgg cgaccaatcc
     3481 cggctgtcct ttcgcaaaac tttcgactgc tatgatgaat tgcgccgctg ccgcaagcgc
     3541 gtcatggcgc aatgccatgg gtgtcgttcc agcgtgtccc gctttgcccg tgaagatagc
     3601 cttgatgcgc gtttgcccgg cgatggcgga aaccacgccc acaggctgtt ttttttcttc
     3661 gagcaccggc ccttgttcga tgtggacttc cgcgtaacca agcaatcggt cagcatcgcg
     3721 ccgcgcggat ttcaattggt cgggattgcc tccgaatgaa cgaatcgctt cacccatcaa
     3781 aatgccattg gcatctgcgc gttgcaaatc gcgttcgttc aatttgccaa ccagcgcctt
     3841 gctgccgagg taagtcattt gatagcgcac gccttcctcg tcagcgaaac ccaccacttc
     3901 caatgcaaac ggcaaatgga ttttttgctc atgcaaatgc ttcacgcacg ctattgcggt
     3961 cagcacgcca agcgggccgt cgaattttcc cgcatcgcgc acggtgtcga ggtgggagcc
     4021 aaggatgaaa cttttggcat gttcgtcgcg cgcgggataa cgaccaatca cgttgccgat
     4081 ggcatcttgc gtgacatcca tgcccgcttc gcgcatccag cccgcgacga gttcgtttgc
     4141 ctgccgcatc gcggtcgagc aaaaggttcg cgtgagttgg cccggttcct cgctgatgcg
     4201 cgccagcgct tcaatccgct ccatcaccgt gcgcgcaagc tcgttatggt tcattcagct
     4261 tccccgataa gtgctgtacg accatggcga cacaagcaac gggacgtgat aagaagcctt
     4321 ggcatcggcg atgccgaagc gcacgggaac ttcgtccaga aatgggaatt cagcgcgcgt
     4381 atcggttttt tcatcgaaat aatcgcccac gtgaaaaacc agttcataaa gcccggcctt
     4441 caattcttcg ccggcgagca gcggttcgtc cgtgcgcccg tcctcgttgg tgcgggcgga
     4501 tttcaagagc ttcggctccg cgtcgtcgag ggaccaaagt tcgatctgca ttccttgcgc
     4561 gggacagcca tttgccgtgt cgagaacgtg cgtgctcaat tttgcaggca taaccaatga
     4621 aacaatgccc ccgcgcgatt gccttagtaa actctaaaaa cttccagaca catgcatcgg
     4681 ggcatcttga cactgccccg gagcgcggct cggtgagccg cagcaacaca ccattatcaa
     4741 gacacgccag actaattcat acacaccggt attcctacgt tgctgcggct caccgagccg
     4801 cgctccgttt tacatggtga acgccgcggg caatagctca accggtctat cgggcatgac
     4861 caacaaattg ggccactgtc agatggccca tgcatcaacc gtgcaaaact atgacggctt
     4921 ggaaccaatc cttgttccat atttgctttg ggtatttgga tgcttgattc cctcctctcc
     4981 tttcactagc ttgcaggaaa ccggcagaag ccatcatcct gtgttttcgc aaaaatccaa
     5041 acccatcatt gagcgttgcg agcgcgacat gaacacgcgc ctgcggctca aatacgagcc
     5101 gtattatcac gtcgtggatg cggttgatgg cgtcaacgtc actgtcgagg ggcgtccgat
     5161 ggtgatgatg tcgagcaacg aatatctcag tttgagccgg catcccaagg tgattgccgc
     5221 cgccaaaaga gcgctcgatc aatggggaac cagtccatgc ggttcgcgcc ttgccaatgg
     5281 cagcaggcgt tatcacatcg agcttgaaga agcactcgcc gcttttctcg ggcaggaatc
     5341 ctgtcatgtc ctttccgcgg gttatctcgc ttgcatttcg agtttgagtt cgctggtcca
     5401 gcgcggcgac acgctcatcg tggatccggg cattcattca tcgctttggg atggcgcgtt
     5461 gttgagcggg gcaaaactcg aacgtttcga acacgaaaac accgcgtcgc tggcaaaagt
     5521 gctcggcgct ctcgacgcgc gtcaggccaa ggcaatcgtc gtggacggcg tttattcgat
     5581 ggaaggccat atcgcatcgc ttcccaaaat ttgcgaactc gcggagcaac atcaggccgc
     5641 gctgatcgtg gatgacgcgc atggcatcgg catcctgggc cgtgaaggcc gcggcgtgtg
     5701 cgaccatctg ggcgtgaccg aacgcgtgga tttgattgtg ggcagctttt ccaaatcact
     5761 cgtcggcacg ggtggctttg ttgccggcag caaggcgctc atcgaatatt tgcgcagcaa
     5821 ttcgcggcag atcattttca gcgccgccat aagtcccgcc gcagccgcag ccgcgctggc
     5881 cgcgctggaa gtttcgcgct ccgaacccga acatcgcgaa cggctctggg ccaattacca
     5941 tcatttgcgc gccat
//
LOCUS       DASZMM010000359         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_138966, whole genome shotgun sequence.
ACCESSION   DASZMM010000359 DASZMM010000000
VERSION     DASZMM010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138966"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VG754_08720"
     CDS             <1..789
                     /locus_tag="VG754_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWF19338.1"
                     /translation="PGSNYKLWTTVKVPQGFSMPKHCEFLLRQTGAEHYRLMPAKRLF
                     ALGVGHVRRRGMAPGSKADVPAEVMDVNVVQLNDQEWRVLTALKREFEPDEITRDIWQ
                     ARADEAGVSLEIFCELAEDLNRRKVIGRFSTFLEHVKPLASGERVTRFNALFHWAVPA
                     GREIEAGREVGRHHILTHAYWREGGPEFRNVNVMGVAHGTDRELVLQHKAAIDEHLRS
                     INIPVSYTNVFWGGRSEIKPSEISPFAYQEWCKSVGLDPDAMRD"
     gene            complement(786..>2398)
                     /locus_tag="VG754_08725"
     CDS             complement(786..>2398)
                     /locus_tag="VG754_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWF19339.1"
                     /translation="LAATLEKLCAGGASRCGEVRRMKSDLHLISRAKIFLLLFALLAA
                     GKVAAAALRFEVTADKKLCDSPVTGRLLVILDRHPMPEPRLGMHADGPDAPFIIGRDV
                     NDLAPGVKAVVDGKCITFPITNLSQLPAADYYVQALLMTNIDIRSPNSYGNLYSELRQ
                     VHLDPSHSSTVKLQLTHRIPGEKIPADTDLIKFIKIQSPLLSKFHGRPIYLRAGIVLP
                     RDFAKETRKYPLWVRIGGWGTRYTMVQALMDKNTPFRKNWMADGTPRMILLQLDGAGP
                     YGDPYQVNSANNGPYGDAITQELIPLVEEKFRGIGKPHARVLSGLSTGGWVSLALQVY
                     YPDFFNGTWTFCPDGVDFRAFELVNIYEDTNTYVNEYGCERPSERAVNGDVRLYMRRE
                     VQFENVLGTGDSWTMSGKDWCAWNAVYGPRGADGRPTALWDPQTGKIDRALAEEWKKY
                     DLRLVMEQNWKTLGPKLQGKLRISVGDADNYFLNNAVHLLDDFLSHADPPYKGRIVFG
                     PGRGHGWTDITTEQQLKEMDAATGGPQD"
BASE COUNT          531 a          672 c          673 g          522 t
ORIGIN      
        1 cccggctcga actacaaact ttggacaacg gtgaaggttc cgcagggttt ctcgatgcca
       61 aagcattgcg aatttctttt gcgccaaacc ggcgcggaac attaccgttt gatgcccgcg
      121 aagcgcttgt ttgcattggg cgtcggacat gtccgccgtc gcggcatggc gccgggcagc
      181 aaggctgacg ttcctgccga agtgatggac gtgaacgtcg tgcaattgaa cgatcaagaa
      241 tggcgcgtgc tcactgcgtt gaaacgcgaa ttcgagccgg acgaaatcac gcgcgacatc
      301 tggcaggcgc gcgccgacga agcgggcgtt tcgctcgaaa tattctgcga gcttgccgag
      361 gacttgaacc ggcgcaaggt catcggacgt ttctccacat ttttggagca cgtgaaaccg
      421 cttgccagcg gcgaacgcgt gacgcgtttt aacgcgctgt ttcattgggc agtcccggcg
      481 ggccgggaga ttgaagccgg ccgggaagta ggccggcatc atatattgac gcacgcgtac
      541 tggcgtgaag gcggtccgga atttcgcaat gtcaacgtca tgggcgtggc gcacggaact
      601 gaccgcgaac tcgtccttca acacaaggcc gcgattgacg agcatctgcg cagcatcaac
      661 atcccggtga gttacaccaa cgtgttctgg ggcggacgca gtgagatcaa gccttcggaa
      721 atttcgccgt tcgcttatca agagtggtgc aaaagtgtgg ggttggatcc agacgcgatg
      781 agggattaat cctgcggccc gcctgtggcg gcatccattt ccttcaattg ttgttcagtg
      841 gtgatgtccg tccagccgtg gcctctgccg gggccgaaca caatcctgcc tttgtacggc
      901 gggtccgcgt gggaaagaaa atcgtcaagc agatggacgg cgttgttcaa aaaataattg
      961 tccgcatcgc caaccgagat gcgcaatttg ccctgcagct ttgggccgag cgttttccaa
     1021 ttttgttcca tgacgagccg caagtcgtat tttttccatt cctcggcaag agcgcgatcg
     1081 attttcccgg tttgcgggtc ccataaagcc gtcgggcgtc catccgcgcc gcgcgggccg
     1141 taaacggcat tccacgcgca ccaatccttg ccggacatcg tccaactgtc gccggtgcca
     1201 agcacattct cgaactgcac ttcgcgccgc atataaagac ggacatcccc gttgaccgcg
     1261 cgttcgctgg gtcgttcgca accatattcg ttgacgtaag tgttcgtgtc ttcgtagatg
     1321 ttcaccagtt cgaaagcgcg gaaatccacg ccatccgggc agaacgtcca tgtgccattg
     1381 aaaaaatcgg gataataaac ctgcaacgcg agcgataccc agccgccggt ggacaaaccc
     1441 gacaatacac gcgcatgcgg cttgccgatg ccccggaact tttcttccac caacggaatc
     1501 aactcttgcg tgatagcatc accataaggg ccgttgttcg cagaattcac ctgataaggg
     1561 tcgccataag gtcccgcgcc atccaattgc aacaaaatca ttctcggcgt tccgtctgcc
     1621 atccaatttt tcctgaacgg cgtgttttta tccatcagcg cctggaccat cgtgtagcgc
     1681 gtgccccaac cgccgatgcg cacccagagc ggatatttgc gcgtctcctt tgcaaaatcg
     1741 cgcggcaaca cgattcccgc gcgcagataa atcggccgcc catgaaactt gctcaaaagc
     1801 ggcgactgga ttttgatgaa tttgattaag tcagtgtcgg cagggatttt ttcaccagga
     1861 atacgatgcg tgagctgcaa cttcaccgtg ctgctgtgcg atggatccag gtgaacctgt
     1921 cgaagttcac tgtaaaggtt gccgtacgag ttcggcgacc ggatgtcaat gttcgtcatc
     1981 agcaacgcct ggacataata atccgctgcc ggaagctggg aaagattggt gatgggaaag
     2041 gtgatgcatt ttccgtcaac aaccgctttg actccgggcg caagatcgtt gacatcacga
     2101 ccgatgatga atggcgcgtc cggtccgtcg gcatgcattc ccaatcgcgg ttcgggcatc
     2161 ggatggcggt caagaatcac cagcaatcgc ccggtgacag gtgagtcgca caactttttg
     2221 tcggcggtga cttcgaagcg caacgcagca gccgctactt ttccggcagc gagcaaagca
     2281 aagagcaaaa gaaaaatttt cgcgcgtgag atcaaatgca agtcggactt catacgccgg
     2341 acttcgccac atcggctggc accgccggcg cataattttt cgagagttgc tgccaggc
//
LOCUS       DASZMM010000360         5715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_961235, whole genome shotgun sequence.
ACCESSION   DASZMM010000360 DASZMM010000000
VERSION     DASZMM010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961235"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1220)
                     /gene="murG"
                     /locus_tag="VG754_08730"
     CDS             complement(45..1220)
                     /gene="murG"
                     /locus_tag="VG754_08730"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWF19340.1"
                     /translation="MQNQVSTPRVAIACGGTGGHLFPGMAVAEELKQRGCSVALLISP
                     KDVDQQAVKSATGMDVATLPAVGLSRGRGLAFMKGFMQSYRAAAKLFKSARPDAVLAM
                     GGFTSAPPVLAGKACGAPAFLHESNTIPGRANRWLSWTVNQAFVGFPEAAKRLHTKAV
                     KFTGTPVRPQFHPMDAATCRTALGLDPNRPMLLVTGGSQGASGLNDVVIQALPLLARQ
                     VPGLQLFHLTGPTDVQKVEKACAAANVKAIVKPFFSEMHLALGAATAAISRAGASSLA
                     ELAAMRVPAILLPYPAATDNHQYYNTLAFEKTGAAFLMAQKNAKAEFLAPKVVELIQD
                     ADTRAKMQSALADWHIPRAAANIAENIMQVVAARRRVRGSVTVPGSPNIDNRQSVIP"
     gene            complement(1350..2474)
                     /gene="ftsW"
                     /locus_tag="VG754_08735"
     CDS             complement(1350..2474)
                     /gene="ftsW"
                     /locus_tag="VG754_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWF19341.1"
                     /translation="MKTATSILVVCVAALLALGMVMLYSSSMADKGGGHLLQMQIVWC
                     SLGLLGCSVAASIDYRKLKIFALPLFVLAVALLVLVMLPHFGSKVNGARRWFRVGGVQ
                     FQPSELAKLALIIAVAWYGDQFQRKLGDFKRGLVFPALLIGTVLGLIFIEPDRGTTIL
                     MAAVSAGMLFVAGARWKYVVPPVILGAAGLAFSLLYDPMRTKRIFAWLHTDEHKMDTG
                     YQAYQAMLALGAGGWTGLGLGNSREKLGFLPEHNTDFIFSIIGEELGLVATLLVVVAF
                     VLLVICGIYISSHASDNFGTLLGCGVTFLIGLQAFINIGVVTSALPNKGLPLPFISYG
                     GSNLLMMLTAVGLLLSIARRARVAPAVATSMEHDEVPQTA"
     gene            complement(2548..3324)
                     /locus_tag="VG754_08740"
     CDS             complement(2548..3324)
                     /locus_tag="VG754_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWF19342.1"
                     /translation="MNNSNPFVPQGSLLEQKNKKRARVKVAVFSIFALNILVITPLLI
                     QGCSKKDTGDQTANQPVDTTPAPDTNTPPPLPQVASNAVPATTNPPMVAEPVAPPPPV
                     APPAPETHEYVITKGDTYYSIAKKFSLKMSDIKAANPAVNPAKLKVGQKIEIPSGASG
                     STGTIAGTDASGDVYVVKSGDSLTKIAKANGVTVKAIRTANELKTDRIKVGQKLKLPM
                     KAAPADTNPVPPSSTTAGTVPIPQPAPTGPAATASNGTVH"
     gene            complement(3321..3725)
                     /locus_tag="VG754_08745"
     CDS             complement(3321..3725)
                     /locus_tag="VG754_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19343.1"
                     /translation="MIVVMRAFENKMILTDAAGKKNPTREILFTPCTTPESLLEAVTM
                     AAQNALPEDVLLLSSACSNFDQFRNHQRRMEPLPAAASRMPSENYFVMQKNEPAIFDL
                     LRGFSEEKTPARKTKQLHSTSQERTLSSAKAK"
     gene            complement(3733..4884)
                     /gene="mraY"
                     /locus_tag="VG754_08750"
     CDS             complement(3733..4884)
                     /gene="mraY"
                     /locus_tag="VG754_08750"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWF19344.1"
                     /translation="MLYYLSQYLIDAAAGTSWESEVSALRLFRYITFRSAGAAVTALL
                     LSLWFGPRVIAWLKELSFGQNYRDRAEVAGNLTSREPGKKFGTPTMGGLLIVLTLNLT
                     TLLWAEWNTLVQLVVLTVVVLAALGFYDDYTKITKASSGGAKSHVKLWVQIALSLFIG
                     CYLWYAPATQKLITDIMVPFYKYPVVSGVGGAGLALTMLTVVGSSNAVNLTDGLDGLA
                     IGCTLIVSFVFLIMTYLAGNLKIAAYLQIPHVSGAGELTVFCAALCGAGLGFLWFNCH
                     PAQVFMGDTGSLALGGALGVIAVLIHQPLVLLIAGGVFVMEAVSVILQTTYFRYTRKR
                     YGTGKRLFLMAPLHHHYEKKNWHETQVVTRFYILCVLFAVLALSSLKIR"
     gene            complement(4885..>5715)
                     /gene="murF"
                     /locus_tag="VG754_08755"
     CDS             complement(4885..>5715)
                     /gene="murF"
                     /locus_tag="VG754_08755"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWF19345.1"
                     /translation="IGREHLEFFGDLAGVAMEEGWLAELLPAGGTLFLNGDSEWTGEI
                     ARRGRAKVWRIGLAIENDWRPGNVQMDEQGMRFWVAGPKPNIAGEYRINLLGRHQVVN
                     AVFAIAIGAELGLTREEIQGGLNECKPAKMRLQRWNWNGVCVLDDAYNANADSMKAAL
                     QTLQEMPCRGRRVAVLGDMGELGGHSRAAHEEAGRIAADMGIDQLFAVGSMAGVMANA
                     ARAAGLQDVDEFSDPVTAADAVKQFLRAGDVMLLKASRATRMERLTEALKSQTAAGKV
                     "
BASE COUNT         1281 a         1773 c         1462 g         1199 t
ORIGIN      
        1 tctccggttc tcaccggctt cggagcaatc tggacttcgt tgcttcacgg tatgaccgat
       61 tgtcggttgt caatgtttgg agaaccgggc acggtcacac tgccgcgcac gcgacggcgc
      121 gcggcgacaa cctgcataat gttctcggcg atgtttgcgg cagcgcgtgg aatatgccaa
      181 tcggccaatg ccgattgcat ttttgcgcgc gtatctgcat cctggatcaa ttcaaccact
      241 ttcggcgcga gaaattccgc tttcgcattt ttttgggcca tcaaaaaggc cgcgccggtt
      301 ttttcgaacg caagcgtatt ataatattgg tgattgtcgg tcgcggcggg atacggaagg
      361 aggatggccg ggacacgcat cgccgccaat tccgccagcg atgatgcccc cgcgcggctg
      421 atggccgcag tcgccgctcc caatgccagg tgcatctcgc taaaaaacgg tttgacaatc
      481 gccttcacgt ttgcggcggc gcaggctttt tcaacttttt gaacatccgt cgggccggtg
      541 agatgaaaca attgcaatcc cgggacttgc ctggccaaaa gtggcaatgc ttgaatgacg
      601 acgtcgttca acccgcttgc tccctgactg ccgccggtga cgagcaacat cggacgattc
      661 gggtccaacc caagggccgt gcggcaggtt gcggcatcca tcgggtgaaa ttgcggacgc
      721 accggtgtgc cggtgaattt taccgctttc gtgtgcaacc gtttcgctgc ttccggaaag
      781 ccaacgaacg cctggttgac ggtccacgac aaccaacgat ttgcgcgccc ggggatggta
      841 ttggattcgt gcaagaatgc cggagcgccg caagcttttc cggccaacac cggcggggcg
      901 ctggtgaatc cgcccatcgc cagcacggca tcgggacgcg cggatttgaa gagtttcgcc
      961 gccgcgcgat acgattgcat gaagcccttc atgaacgcca gacctcggcc gcgactcaag
     1021 ccgactgccg gcaatgtcgc cacgtccatg cccgttgccg atttcacggc ttgttggtca
     1081 acgtccttgg gagaaatcag caaagcgacc gagcaacctc gttgtttcaa ttcctccgca
     1141 acagccattc cgggaaacag atgccctccc gtgccaccgc aagcgatggc aacccgtggc
     1201 gttgaaacct gattttgcat taaaaatttc caagaaacat cgaacgctga acatccaacg
     1261 tccaacatcg aattggactt caaaccaacc ctctgcaatc agcgttcgac gttcaatgtt
     1321 cagcgttcag tgtttctgtc ccgtattgct caagcggttt gcggaacctc gtcgtgttcc
     1381 atcgacgtcg cgaccgctgg cgcaactcgc gcgcgccgcg cgatgctcaa aagcaaaccc
     1441 acagccgtca gcatcatcaa cagattggac ccgccataac tgatgaacgg cagcggcaga
     1501 cctttgtttg gcaatgcgct ggtcaccacc ccgatattga tgaacgcctg cagaccaatc
     1561 aaaaatgtga cgccgcaacc gagcagcgtg ccgaaattat cgctcgcatg tgaggagata
     1621 tatattccgc aaatcaccag caggacgaac gccaccacca ccagcagcgt cgcaaccaac
     1681 cccagttcct cgccgatgat tgaaaaaatg aaatcggtgt tgtgctccgg caggaagccg
     1741 agtttttcgc ggctgttgcc caagcccagc ccggtccatc cgcccgcgcc gagtgccagc
     1801 attgcctgat acgcttgata gccggtgtcc atcttatgct cgtccgtgtg caaccatgca
     1861 aaaatgcgct tcgtccgcat cggatcgtac aaaagcgaga acgcaagacc tgcggctccc
     1921 aggatgaccg gcggaacgac gtatttccaa cgcgcaccag cgacaaacaa cattccggcc
     1981 gagactgccg ccatcaaaat ggttgtgcca cggtccggct cgatgaagat caatccgagc
     2041 accgtgccta tcagcagcgc cggaaaaacc aaaccgcgtt tgaagtcgcc cagcttgcgt
     2101 tgaaattgat ccccgtacca ggcgacggcg atgatcaagg ccagctttgc cagttccgaa
     2161 ggctgaaatt gcacgccccc aacccgaaac caacggcgcg cgccgttcac cttcgagccg
     2221 aaatgcggaa gcatcaccag cacgagaaga gcaaccgcca gcacaaaaag cggcagcgcg
     2281 aatattttta attttcgata atcaatgctg gccgccacgc tgcaaccgag caggccaagc
     2341 gagcaccaga cgatttgcat ctgcaacaga tgcccgccgc ccttgtccgc catgctcgag
     2401 ctgtaaagca tcaccatccc cagcgctaac agggcggcga cacaaacaac caaaattgaa
     2461 gtggcggtct tcatttcaaa ccatgctcaa cttgccgcca gagaccgggt tgaggtcgcc
     2521 cggcatctgg ccagcaaatt gtttcaatca atgcactgtt ccgttcgagg cggtcgccgc
     2581 cgggcctgtc ggggcgggct gcggaatcgg gaccgttccc gcggtggtgg acgaaggcgg
     2641 aaccggattc gtgtccgctg gcgcggcctt catcggcaat ttcagttttt gaccaacctt
     2701 gatgcggtcg gtcttcaact cgttcgcggt tctaatggcc ttgaccgtga cgccgttcgc
     2761 cttggcgatc tttgtcaacg aatcaccgga cttgaccaca taaacatcac cgctggcatc
     2821 agtgcccgca atcgttcccg tcgaaccgga agcaccggac ggaatctcaa ttttctggcc
     2881 gaccttcaac ttcgccggat tcacggccgg attggccgcc ttgatgtcgg acatcttgag
     2941 gctgaatttc ttggcgatgg aataataagt gtcgcccttg gtgatcacat attcgtgtgt
     3001 ctcaggagcg ggtggcgcaa caggaggagg cggagccacc ggctcggcga ccataggagg
     3061 attggtcgtg gccggaaccg cattgctggc gacctgcggc aacggcggag gagtgttggt
     3121 gtccggtgcc ggagtggtat cgaccggctg gttggcagtc tgatcgcctg tgtccttctt
     3181 gctgcaaccc tggatgagca acggggtgat gacaagaatg ttgagagcga agatggaaaa
     3241 aacggcgacc ttgacgcggg cgcgtttttt atttttttgc tccaacaggg agccttgtgg
     3301 gacgaatgga ttcgagttgt tcatttagct ttggcgcttg agagcgtcct ttcttgtgag
     3361 gtggagtgga gttgtttggt ttttcgcgcc ggggtttttt cctcagaaaa accccggagc
     3421 aaatcaaaaa tggccggttc gtttttttgc atcacaaaat aattttcgga cggcattctg
     3481 cttgccgccg caggcaacgg ttccatgcgg cgctgatgat tccggaactg atcgaagttc
     3541 gagcaggctg atgaaagcaa aagcacatct tcgggcaacg cattttgcgc ggccatggtg
     3601 actgcttcta acagtgattc cggcgtggtg caaggagtaa aaagaatttc cctcgtagga
     3661 ttttttttac ccgccgcgtc agtcaaaatc atcttgtttt caaacgctcg catcacaaca
     3721 atcattttct tttcaacgta ttttcaagct gcttaaagcc agcacggcga acagcacgca
     3781 caggatgtaa aagcgcgtca ccacctgcgt ctcgtgccag ttcttcttct cgtaatgatg
     3841 gtgcaacggc gccatcaaaa acaatctttt ccccgtcccg taacgtttcc gcgtatagcg
     3901 aaagtatgta gtctgcaaaa tcaccgacac cgcttccatg acgaacactc ctcccgcaat
     3961 caaaagcacc agcggctgat gaatcagcac cgcaatcact cccagcgcgc cgcccagcgc
     4021 gagggacccg gtgtcaccca tgaacacctg tgccggatgg cagttgaacc agagaaaacc
     4081 aagacccgct ccgcacagcg ccgcgcaaaa aaccgtcagc tcgcccgcgc ccgacacatg
     4141 cgggatttgc aggtaagcgg cgatttttaa attgccggcc aggtacgtca tgatcaaaaa
     4201 aacaaaactc acgatcagcg tgcaaccgat ggcaaggccg tcaaggccat ccgtcaggtt
     4261 caccgcgttc gaactgccca ccaccgtcaa catcgtcaat gccagccccg cgccccccac
     4321 tccgcttacg accgggtatt tgtaaaacgg caccatgatg tccgtgatca atttttgcgt
     4381 cgcaggcgca taccaaagat aacaaccgat gaacaacgac aacgcgattt gcacccaaag
     4441 cttcacgtgc gacttcgccc cgccgctgct cgccttggtt atcttggtgt aatcatcgta
     4501 aaaaccgagc gcggccagca ccacgaccgt caacaccact aactgcacca aagtgttcca
     4561 ttccgcccaa agcaacgtcg tcaaattcag cgtcagcacg atcaacaaac cgcccatcgt
     4621 cggcgtgccg aatttttttc ccggctcgcg cgaggtcagg ttgcccgcga cttcggcgcg
     4681 atcgcgataa ttttggccga agcttagttc cttcaaccac gcaatcaccc gcggcccaaa
     4741 ccaaaggctc aagagcaacg ccgtgaccgc agcgcccgcg ctgcgaaaag tgatgtagcg
     4801 gaacaatcgc aatgccgata cttcgctttc ccacgatgtt cccgccgccg catcaatcaa
     4861 atattggctc agataataaa gcattcagac ttttcccgcc gctgtttgcg atttgagcgc
     4921 ttctgtcagc cgctccatcc gcgtggcccg cgacgccttc aacagcatca catcgcccgc
     4981 ccgtaaaaat tgtttgaccg catccgccgc cgtcaccggg tcggaaaact catccacatc
     5041 ctgcaatccc gccgcccgtg ctgcgttcgc catcactccc gccatgcttc ccaccgcaaa
     5101 aagctggtcg atgcccatgt ccgccgctat ccgccccgct tcttcatgcg ccgcccggct
     5161 gtgaccgccc aattcgccca tatcgcccaa aaccgccacc cgcctgcccc ggcaaggcat
     5221 ctcttggagg gtttgcaaag cggccttcat cgagtccgca ttggcgttat aggcatcatc
     5281 aagcacgcat actccgttcc aattccagcg ttggaggcgc atttttgcag gtttgcactc
     5341 atttaatccc ccttggatct cctcccgggt caaacccagc tcggccccaa tcgcaatggc
     5401 aaagaccgca ttcacaacct gatgtcgccc aaggagattt atacggtatt cccccgcaat
     5461 attcggtttt ggaccggcca cccagaaccg catcccctgc tcgtccatct ggacattgcc
     5521 cggacgccag tcgttttcga tggcgaggcc gattcgccac accttcgcac gaccacgccg
     5581 ggctatttcc ccggtccatt cgctgtcccc gttcaggaaa agcgtcccgc cagccggcaa
     5641 aagctccgcc agccagcctt cctccatggc gactcctgcc aaatctccaa aaaattccaa
     5701 atgttcgcgc ccaat
//
LOCUS       DASZMM010000361        10248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4283536, whole genome shotgun sequence.
ACCESSION   DASZMM010000361 DASZMM010000000
VERSION     DASZMM010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4283536"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /gene="hypF"
                     /locus_tag="VG754_08760"
     CDS             complement(<1..1188)
                     /gene="hypF"
                     /locus_tag="VG754_08760"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HWF19346.1"
                     /translation="MNCERAKIMVRGTVQGIGFRPFVYKLATELGLNGWVLNSSQGVQ
                     IEVEGARAMLDSFLQRLEKEKPPHAAIHGVEHSLLDAVGHAGFEIRDSDDEGSKTACV
                     PPDLATCADCLREIFDPRNRRYLYPFTNCTNCGPRFTIIEALPYDRCHTSMKQFTMCA
                     ECEAEYQNPLDRRFHAQPNACPKCGPQLAFWDETGKELAEGGDVLKQAVMAIRSGSIV
                     ALKGMGGFQLMVDARNETAVMRLRQRKRREEKPFALMYPSLSALKRDCRVSELEARLL
                     LSSQAPIVLLERNTSLSTVSSSVAPGNPTLGAMLPYTPLHHLFMRDLGFPVVATSGNL
                     ANEPICIDENEALERLLGIADFFLVHDRHIVRHMDDSIVRVMLGREMVLRRARGYAPS
                     PIRL"
     gene            complement(1189..1878)
                     /locus_tag="VG754_08765"
     CDS             complement(1189..1878)
                     /locus_tag="VG754_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity nickel-transport family protein"
                     /protein_id="HWF19347.1"
                     /translation="MRHATDADHVVAVTTIVSRERSISHAAWIGAFWGIGHTLTLLIA
                     GGAIIVFGIVVPNRLGMSLEFSVALMLILLGALNFRNFMRSVNSTTAAARHHHSHTAE
                     METEEHALERRSNWFDKKFKRIGFYQTLRPLVIGVVHGLAGSAAVALLVLPLIHNPVW
                     AFAYLLIFGGGTIGGMMLITAAMALPISFTARRSAAFHRRIGFAAGFLSVAFGLFLVY
                     QIGFVDGLFTK"
     gene            complement(1931..2548)
                     /locus_tag="VG754_08770"
     CDS             complement(1931..2548)
                     /locus_tag="VG754_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWF19348.1"
                     /translation="MLLDTTTESKNAPVIPRLNGDEKHEPGAMDELNEQARRIQELME
                     QVDALPDLAARALLHECLETVLSLQGRGLAQILQIVQDAGPEGQKVLDGLIANDAVRA
                     ILLIHGLHPVDLATRLQQALDKVRPYMESHGGNVELLSLENDVARLRLHGTCKSCPSS
                     AVTMELAVRRAIEEFCPDLAGFEVEGAGGEPISNDATVGDGKMQH"
     gene            complement(2538..2909)
                     /locus_tag="VG754_08775"
     CDS             complement(2538..2909)
                     /locus_tag="VG754_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HWF19349.1"
                     /translation="MHELSVVSGLVESVLEFVESHAVKRVAAVRLQVGELAQLEVEQL
                     RFCYEAMIEKTAMEGSTLEIEPISAEVNCAHCSYHGPPKYWEGALSGTLVPTLQCPTC
                     GHAAEAVRGHECSIKSIQYVA"
     gene            complement(2902..3597)
                     /gene="hypB"
                     /locus_tag="VG754_08780"
     CDS             complement(2902..3597)
                     /gene="hypB"
                     /locus_tag="VG754_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HWF19350.1"
                     /translation="MIPTIQASPEETYDIELEHSLLQANAKIAQENRARLDRHDITAI
                     DFMGAIGSGKTTLITRMTEKLRDQLRLAVFNGDATTVDDFNLVAETGVPTVQLATVNG
                     CHLDANLVAKALQKIDLENTDLIFIENIGNLICPAEFPLGSRSRVVVVSVTEGPYMVK
                     KHPHMFLGADIVVINKTDLAEMMGVKIAALAQDVHSLKPDIKVIPTSCKTGAGLDEVA
                     AALLATANVPLHA"
     gene            complement(3629..5443)
                     /locus_tag="VG754_08785"
     CDS             complement(3629..5443)
                     /locus_tag="VG754_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HWF19351.1"
                     /translation="MAEAIATKPRQVKPAESGNLVEMSWDPITRIVGSLGIFTKIDFD
                     KKKVAECHATSSIFRGYSIFMQNKDPRDTHFITSRICGICGDNHATCSIYAQNMAYGI
                     KPPPLAEWIINLGEAAEYMFDHCIYQDNLVGVDFCEMMVKETNPGVWEKAKKKEAPHA
                     KDHGYKTIADIMTSLNPFEGEFYRKTLQMSRLTREMFCLMEGRHVHPSTLYPGGVGTV
                     PSIQVFTDYLTRLMTYIEFMKAVVPLHDDLFDFFYEAMPGYEKVGQRRILLGCWGSWQ
                     DPDVCDYKYRTMNEWGKAMFVTPGVIVDGELVTTNLVDINLGIRILLGSSYYEDWKEN
                     GKTFVKYDPLGNPVDQLHPWNQTTTPKPQKRNFDDKYSWVMSPRWYDKRTGDYLALDT
                     GGGPIARLWSTALAKLVDIGYVKATGNSVKIRLPKTATKPEVELEWKIPQWSNAIERD
                     RARSYFQAYAAAVGLYCVEQAMKEVYAGRTETFTPFKVPEEGIGCGFHEAVRGVLSHH
                     LVIEDKKVANYHPYPPTPWNASPRDIYGTPGPYEDAVQNTPIFEENGPDKFKGIDIMR
                     AVRSFDPCLPCGVHCYLGDGKMLKQMHTPTAFASLAKK"
     gene            complement(5458..6558)
                     /locus_tag="VG754_08790"
     CDS             complement(5458..6558)
                     /locus_tag="VG754_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HWF19352.1"
                     /translation="MKTPEVVADRPPAVKEVHVVWMTSGLSCDGDSISTTAATLPSIE
                     DVVMGNIPGLPKVHLHNPVLAYEVGDDFMKYWHMAAEGRLEPFVLVLEGSVPNEEIKA
                     EGYWAAMGTDPHTGQPITTNEWIDRLAPQATAIICAGTCATYGGIHAMQGNPTGAMGL
                     ADYLGWDWRSKAGLPIVNVPGCPVQPDNMMETVLYLLYQLAGLAPMIPLDDQLRPTWL
                     FGKTVHEGCDRGSYYEQADFASEYGSPKCLVKIGCWGPVVNCNVTKRGWMDGIGGCPN
                     VGGICIGCTMPGFPDKFMPFMDAPPGSALSTAVVGTWGGVIRKLRAITNHTCNKEPKW
                     HHRGTRLTTGYKPKYYGNGRNIGDGNGNGNRS"
     gene            complement(6758..7315)
                     /locus_tag="VG754_08795"
     CDS             complement(6758..7315)
                     /locus_tag="VG754_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HWF19353.1"
                     /translation="MRILIAGIGNIFQGDDAFGVETVRRLSELPLPEGVRVVDFGIRS
                     YDLAYAIMEGYDIVILVDATPRGEVPGTVYLMELEWGSAGQFGSEGANAHGLNPVAVL
                     QMVQSLGGKSGGRIYLVGCEPAVLETENGELGLSPAVQAAVPEAIQLIQSLLNNLICG
                     DHQQAESETLNEEFVPCETTTQEKL"
     gene            complement(7317..8693)
                     /locus_tag="VG754_08800"
     CDS             complement(7317..8693)
                     /locus_tag="VG754_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19354.1"
                     /translation="MPELEEIATGTRPEGEQRTTRLDQIVNAVLYEGYILYPYRPSSK
                     KNQQRFTFGRVYPQAYSAAQSGLEPFVMQTECVARVKPGVSKLKVSARFLQPMAREVG
                     KVGAQENGSFFQIVPELRVDGQLFQTWQEAVEREIKAFLVELKGEIKLNFPFSFPASQ
                     NMELLCDKGGRVVGKLRRRQDAIKGTIEIAVKPLDEEIFKITVRVLNQSPIPKRELND
                     QEAVLMRTFASAHTVLRLEGGEFVSLTDPPNDCQQAAAGCQNLGTWPVLVGDESKGER
                     DTLLSSPIILPDYPQIAPESNGDLFDGTEIDEILTLRIQTMTDEEKMEMRTVDEHARR
                     LLERADNATGQDMLKLHGVMREGRSFDDQIFGTSKPLEGVSFGETYLQPGDKVRICPK
                     SRADVMDMALAGKTGIIEAIEQDAEDRVQLGLVLQDDPGMDLGMLRQPGHRFFYTLDE
                     IEPLEKGE"
     gene            complement(8686..9345)
                     /locus_tag="VG754_08805"
     CDS             complement(8686..9345)
                     /locus_tag="VG754_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HWF19355.1"
                     /translation="MNPKLSTGQGNPFAALRRLARPRAAREHCELCNMAVGPEHRHLL
                     ERSSRKVICACDACAMRFEGVIGGRFKLIPRQPHALPDFHLADEQWESLALPINLAFF
                     FHRTPEDKIIAYYPSPAGATESLLPLTTWEALVADNPVLHRMQPDVEALLVNRVGTAR
                     HYFLAPIDTCYELVGLIRVHWRGLSGGEEVWAEISTFFTKLAERAAPLYSHARREVIH
                     A"
     gene            complement(9363..9563)
                     /locus_tag="VG754_08810"
     CDS             complement(9363..9563)
                     /locus_tag="VG754_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19356.1"
                     /translation="MQTSINRPPAGIHVAGTPRGEEKALKQREPGRNPHRKYYRSARD
                     STGINPANREPIDRSMPEIPPA"
BASE COUNT         2241 a         2982 c         2713 g         2312 t
ORIGIN      
        1 caggcggatt ggcgatggcg catagccgcg ggcgcggcgc aacaccattt cgcgcccgag
       61 catcacgcgc acgatggaat catccatgtg acgcacgatg tggcgatcat ggaccaggaa
      121 aaaatcggcg atcccaagca agcgctccaa tgcttcgttt tcatcaatgc aaatcggttc
      181 attcgcaagg ttgccgctgg tggcaacgac cggaaatccc aagtcgcgca tgaagagatg
      241 atgcaacggc gtgtatggca gcatcgcgcc cagagttgga tttcccggag cgacggatga
      301 cgagaccgtt gataaacttg tgttgcgttc caacaacaca atcggcgctt gagaagaaag
      361 cagcaatcgc gcttccagct cggacacccg gcaatcgcgt ttgagcgctg atagcgatgg
      421 gtacatcagc gcaaatggtt tttcctcgcg ccgcttgcgt tgacgcaatc gcatcaccgc
      481 cgtttcgttc cgcgcatcga ccatcaattg aaaccctccc atccctttca atgccacaat
      541 gctgccggag cggatcgcca tgactgcctg tttcaagacg tcgccacctt cggcgagttc
      601 cttgcccgtt tcatcccaaa acgccaattg cggcccgcac ttgggacatg cattgggctg
      661 cgcatgaaaa cgccgatcca acggattctg atattccgcc tcgcattcgg cacacatggt
      721 gaactgcttc attgacgtgt ggcagcggtc gtaaggaagc gcctcgatga tggtgaatct
      781 cggcccgcaa tttgtgcagt tcgtgaacgg atacagatac cggcgattcc gcggatcgaa
      841 aatttcgcgc aaacaatcgg cacaggtcgc gaggtcgggc gggacacaag cggtcttgct
      901 gccttcgtca tcactgtccc gaatttcaaa accggcatga ccgacggcat caagcaacga
      961 atgttcaacg ccatgaatgg cggcgtgagg cggtttttcc ttttccagcc gttgcagaaa
     1021 cgaatcgagc atcgcgcggg cgccctcgac ttcgatctgg acgccctgcg aagaattgag
     1081 cacccagcca ttcagcccca attccgtggc aagcttgtac acaaaaggcc ggaagccgat
     1141 gccttgcacc gtgccgcgca ccatgatctt tgcccgctca cagttcattt atttggtgaa
     1201 aaggccgtcc acaaaaccga tttgataaac aaggaaaagt ccgaatgcga cgctgagaaa
     1261 accggccgca aaaccaatcc tgcgatggaa tgccgccgag cgccgagcgg tgaaactgat
     1321 gggcaacgcc atcgccgctg tgatgagcat catcccgcca atcgttccac cgccgaaaat
     1381 caacagataa gcaaacgccc aaactggatt atgtatcaac ggcaatacca gcaacgccac
     1441 cgccgccgat cctgccagcc catgcaccac gccaatcacc agcggccgca atgtctggta
     1501 aaacccgata cgtttgaatt tcttgtcaaa ccagttggaa cgccgttcga gcgcgtgttc
     1561 ttccgtttcc atttcagcgg tgtgggagtg atggtggcgc gctgcggcgg tcgttgaatt
     1621 gacggaacgc atgaagttcc tgaaattcag cgcgccaagc aaaatcagca tcaacgcaac
     1681 ggaaaattcc aacgacatcc caagccggtt tggcaccacg atgccgaaaa caatgatcgc
     1741 gcctcccgca atcaacaagg tcagcgtgtg gccaatcccc caaaacgcgc cgatccaggc
     1801 cgcatgactg atcgagcgct cgcggctgac aatcgtggtg acggcgacga catggtccgc
     1861 gtccgttgcg tggcgcatcc ccaaaaaaaa tcccaaaaac aaaaatgaaa acggaacaat
     1921 cataatcacc tcaatgctgc attttcccat ctccaaccgt ggcatcattt gaaatcggtt
     1981 ctccgcccgc gccttccact tcaaatcccg ccaggtcggg acaaaattct tcaatggcgc
     2041 gacgtaccgc cagttccatc gtcaccgccg aggatggaca acttttgcaa gtgccgtgca
     2101 atcgcaaccg cgccacatca ttttcaaggc tgagcaactc gacgtttcca ccgtggcttt
     2161 ccatgtaagg ccgcactttg tccagcgcct gctgcaaccg ggtcgccaaa tcaaccgggt
     2221 gcagaccatg gatgagcaag atcgcgcgca cggcgtcatt cgcaatcaat ccgtcgagca
     2281 ctttttgtcc ttcagggcct gcgtcttgca cgatctgcaa aatttgggca agcccgcgac
     2341 cctgcaaaga caacaccgtt tcaaggcact cgtgcaacaa cgcccgcgcg gcaagatcag
     2401 gcagcgcgtc aacctgttcc atcaattcct ggatgcgacg cgcttgttcg ttcaactcgt
     2461 ccatcgcgcc gggttcatgt ttttcatcgc cgttcaaacg gggaatgacc ggcgcgtttt
     2521 ttgattcagt ggttgtatca agcaacatat tggatggatt tgatggagca ttcatgaccg
     2581 cgaacggctt cagcggcgtg gccgcacgtc gggcattgca acgtcgggac cagcgtcccg
     2641 gacaaggcgc cttcccaata ttttggcggc ccgtggtacg aacaatgcgc gcaattcact
     2701 tccgcggaaa tcggctcgat ttccagcgtt gaaccttcca tggcggtttt ttcaatcatg
     2761 gcctcgtaac agaagcgcag ttgttcgact tcaagctgcg ccaactcgcc cacctgcagg
     2821 cgcactgcgg cgactctctt gacggcgtgc gactcgacaa attcgagcac gctttccacc
     2881 aacccggaca ccactgacag ttcatgcatg aagaggcacg ttggccgttg ccagcaatgc
     2941 agccgcgact tcatccaaac ccgcgccggt cttgcagctc gtggggatca ccttgatgtc
     3001 cggcttcaac gaatgaacgt cctgcgccaa tgcggcgatc ttcacgccca tcatttccgc
     3061 aaggtcggtc ttgttgatga cgacgatgtc cgcgcccaaa aacatgtgcg gatgtttctt
     3121 caccatgtaa ggcccttccg tcacgctcac caccacgacg cggctacgcg atccaagcgg
     3181 aaattccgcc gggcaaatca aattgccgat gttctcgatg aaaatcaggt cggtgttttc
     3241 gagatcaatc ttctgcagcg cctttgccac aaggttcgca tcgagatggc aaccgttgac
     3301 cgtcgcaagt tgcaccgtcg gaacgcctgt ttcggccacg aggttgaaat catccactgt
     3361 cgtcgcgtcg ccattgaaca cggcaagcct gagctggtcg cgaagctttt cggtcatgcg
     3421 cgtgattaag gtcgttttgc ccgaaccgat cgcccccatg aaatcaatgg cggtgatgtc
     3481 atgccggtcc aatcgcgcgc gattttcctg cgcgattttg gcattggcct ggagcaggct
     3541 gtgttcgagt tcgatgtcgt aagtctcttc gggtgaggct tggattgtag gtatcatgtt
     3601 ggatttgatt tatcgttgat ttcgtcagtt attttttggc cagtgaggca aaagcggtcg
     3661 gcgtgtgcat ttgtttgagc atttttccat cgccgagata acaatggaca ccgcatggca
     3721 aacagggatc aaaactgcgg actgcccgca tgatgtcgat gcccttgaat ttgtccggcc
     3781 cgttttcctc gaagatgggc gtgttttgca cggcatcttc gtaaggtccg ggtgtgccgt
     3841 agatgtcgcg cggactggcg ttccatggcg tcggcggata aggatggtaa ttggcgactt
     3901 ttttgtcctc gatgacaagg tggtgcgaaa gcacgccgcg caccgcttcg tggaacccgc
     3961 aaccaatccc ctcctccggc actttgaagg gcgtgaatgt ttccgtccgg cccgcataga
     4021 cttccttcat cgcctgctcc acgcaataaa gcccgaccgc cgccgcgtac gcctggaaat
     4081 aggaacgcgc gcggtcgcgt tcaatggcgt tgctccattg cggaattttc cattccaatt
     4141 cgacttccgg ttttgtcgcg gtcttgggca ggcggatttt gacgctgttg ccggtggctt
     4201 tcacgtagcc tatgtccacc aacttcgcga gcgcggttga ccaaaggcgc gcgatcggtc
     4261 cgccgccggt atccagcgcg aggtaatccc cggtgcgttt gtcgtaccaa cgcggcgaca
     4321 tgacccagga atatttgtcg tcgaaattcc gcttctgcgg cttgggcgtg gtggtctggt
     4381 tccatggatg caactggtcc actggattgc cgagcgggtc atatttgacg aacgtttttc
     4441 cgttctcctt ccaatcttca taataagacg aacccagcag gatgcggatg ccaagattga
     4501 tgtccacgag gttggtcgtg acgagttcgc catcaacaat cacacccggc gtcacgaaca
     4561 tggccttgcc ccattcgttc atcgtccggt atttgtaatc gcacacgtcc gggtcctgcc
     4621 acgagcccca gcaaccgagc agaatgcggc gttgccccac tttttcataa cccggcattg
     4681 cttcgtaaaa gaaatcaaaa agatcgtcgt gcaacggcac cacggccttc atgaattcga
     4741 tgtaggtcat cagccgcgtg agataatcgg taaaaacctg gatggacggc accgtgccga
     4801 cgccgcccgg ataaagagtc gaaggatgca catggcggcc ttccatgagg caaaacattt
     4861 cgcgcgtcag ccggctcatc tgcagcgtct tccggtagaa ttcgccttcg aacggattga
     4921 gcgaggtcat gatgtcggca atggttttgt aaccatggtc tttcgcgtgc ggggcttcct
     4981 tttttttcgc tttctcccaa acgcccggat tcgtttcctt gaccatcatt tcgcagaaat
     5041 ccacacccac caggttgtcc tgatagatgc aatgatcgaa catgtattcg gcggcttcgc
     5101 caaggttgat gatccattcg gccaagggcg gcggcttgat gccgtaagcc atgttctgcg
     5161 cgtaaatgga acaggtcgcg tgattgtcgc cgcagatgcc gcagatgcgg ctggtgatga
     5221 aatgcgtgtc gcgcgggtcc ttgttctgca tgaaaatgct gtacccgcga aaaatggaag
     5281 acgtggcgtg gcactcggcg acttttttct tgtcgaaatc aatcttggtg aagatgccca
     5341 ggctgcccac gatgcgcgtg atcgggtccc acgacatttc caccagattg cctgattcag
     5401 cgggcttgac ttgtctcggt ttggttgcaa ttgcttctgc catataaatc tcctttccta
     5461 actgcggtta ccgttcccat tgccatcgcc gatattgcgg ccgttgccgt aatatttggg
     5521 cttgtacccg gtcgtgagcc gggtccctcg atggtgccat tttggttcct tgttgcacgt
     5581 gtgattggtg atggcgcgaa gtttgcggat caccccaccc caagtaccga cgacggcggt
     5641 tgaaagcgct gaacccggcg gcgcatccat gaatggcatg aacttgtccg gaaagccggg
     5701 catcgtgcaa ccgatgcaga tgccgccgac gtttggacat ccgccgatgc cgtccatcca
     5761 gccgcgtttg gtgacgttgc aattgaccac cggaccccag cagccaatct tgacgaggca
     5821 tttgggcgaa ccgtattcgg aggcgaaatc ggcctgttca taataactgc cgcggtcgca
     5881 gccttcgtgg acggtcttgc cgaaaagcca ggtgggccgc aattggtcat ccagcggaat
     5941 catcggggcg agcccggcga gttgataaag cagataaagc accgtctcca tcatgttgtc
     6001 gggttgcacc gggcaaccag gcacattcac gatgggcagc cccgccttcg aacgccaatc
     6061 ccagccgaga taatccgcca atcccatggc gccggtcggg ttgccttgca tcgcgtgaat
     6121 tcctccatac gtcgcgcaag tgccggcgca aatgattgcg gtggcctgcg gcgcaaggcg
     6181 gtcgatccac tcgttggtcg tgatcggctg gcctgtgtgc gggtcggtgc ccatcgcggc
     6241 ccagtaacct tcggctttga tttcctcgtt cggaaccgag ccttccagca cgagaacgaa
     6301 tggctccaaa cggccttccg cggccatgtg ccaatatttc atgaaatcat cgccgacttc
     6361 ataggcgagg acgggattgt gcagatgcac tttcggcaat ccgggtatgt tgcccatgac
     6421 gacatcctca atgctcggca gcgtggccgc tgtggtggaa atgctgtctc catcgcaact
     6481 cagcccgctg gtcatccaaa cgacgtgaac ttctttgacg gccgggggac ggtccgccac
     6541 cacctctggt gttttcataa atccaataat cctttcttcc tagaaccagt tttcctgccg
     6601 gcagtcttca agaccgccgc cggaacaatc ttcaatttgc tccatcaccg actgcattgc
     6661 tctgtcatcg cctacatgcc gcggatgcgc atataacgcc gcaattctgg aaaattcgcg
     6721 ataaatgccg ccgctgccgc cagcaatgag agcgtgatta caatttttct tgtgttgttg
     6781 tttcgcatgg gacgaactcc tcgttcaggg tttccgattc agcctgttgg tggtcaccac
     6841 aaatcaaatt attcaggagc gattggatca gttgtatggc ctcgggcacg gcggcctgga
     6901 cagcgggact cagcccaagc tcgccattct ctgtttccag aacagccggt tcgcaaccga
     6961 cgagataaat ccgtccgccg gattttccgc caagcgattg caccatttgc aaaaccgcaa
     7021 ctggattcaa gccatgcgca ttggcgcctt cagatccgaa ttgacccgcc gagccccatt
     7081 ccaattccat caaataaact gtgcctggca cttctccgcg cggcgtcgca tccacgagaa
     7141 tgacgatgtc gtagccttcc atgatggcgt aggccagatc atagctgcgt atcccgaaat
     7201 ccacgacgcg cacaccctcc ggcaacggaa gctccgaaag ccgccgcact gtttccacgc
     7261 caaacgcatc atccccttga aaaatatttc cgatgcctgc gatgaggatt cgcatattat
     7321 tctccttttt ccaacggttc gatttcatcc agcgtgtaga aaaaacgatg ccccggctgg
     7381 cgcaacattc ccaaatccat tcccggatcg tcctgtaaca ccagccccag ttgcacgcgg
     7441 tcctcggcat cttgttcgat cgcctcgatg atgccggtct tgcccgcgag cgccatgtcc
     7501 atcacatccg cgcggctctt ggggcagatg cgaactttgt cgcccggctg cagataggtt
     7561 tccccgaacg aaacgccttc caaaggtttc gacgttccga aaatctgatc gtcaaaagaa
     7621 cggccctcgc gcatcacgcc atgcagcttg agcatgtcct gtccggtggc attgtccgcc
     7681 cgctcaagca accggcgcgc atgttcatcc accgtgcgca tttccatttt ttcctcgtcg
     7741 gtcatcgtct ggatgcgcag ggtgaggatc tcatcaatct ccgttccgtc aaaaaggtcg
     7801 ccattacttt ccggcgcgat ttggggataa tctggaagga tgatgggaga cgaaagcaat
     7861 gtgtcgcgct cgccctttga ctcatcgccc acgagcaccg gccatgtgcc gaggttttgg
     7921 caacccgccg cggcctgctg gcaatcgttt ggcgggtccg tcagcgaaac aaattcgccg
     7981 ccttccagtc gcaacaccgt gtgcgcagaa gcaaaagtgc gcatgagaac cgcttcctga
     8041 tcgttcaatt cccgcttcgg gatgggcgac tgattcagga cgcgcacggt gattttaaaa
     8101 atctcctcat ccaggggttt cacggcgatt tctatcgtcc ccttgatcgc atcctgccgt
     8161 cttcgcaatt tccccacaac acggccccct ttgtcgcaaa gcaattccat attttgcgaa
     8221 gccgggaagg aaaacgggaa gttcagtttt atctcgccct tcagctcgac aaggaaagcc
     8281 ttgatctcgc gttcaaccgc ttcctgccag gtttgaaata attgtccatc aacccgaagt
     8341 tctgggacaa tttgaaaaaa tgatccgttt tcctgcgcgc ccaccttgcc gacctcgcgt
     8401 gccatcggtt gcagaaaacg cgcgctgacc ttgagcttgg acacgcccgg tttgacgcgc
     8461 gcaacgcact ccgtctgcat gacaaacggt tccaatccgg attgcgccgc gctgtaggcc
     8521 tgcggataaa cccggccaaa ggtgaatcgc tgctggtttt tctttgaaga aggccggtac
     8581 ggataaagga tgtagccctc gtagaggacc gcgttcacga tttgatccaa ccgcgtggtt
     8641 cgctgctctc cttccggtcg cgtcccagtc gcgatttctt ccagttcagg catgaatcac
     8701 ctcccgcctc gcgtgtgaat acaacggcgc ggcgcgttca gcgagcttcg tgaaaaatgt
     8761 ggaaatctcc gcccaaactt cctctccgcc ggacaatcct cgccaatgca cacggatcaa
     8821 accgaccagt tcgtaacagg tgtcaatcgg cgccagaaaa taatggcgcg ccgttcccac
     8881 ccggttcacc agcaacgcct caacatccgg ttgcattcgg tgcaacaccg gattgtcggc
     8941 caccaaagcc tcccaggttg tcaaaggcaa caaggattcc gtggcgccgg cggggctggg
     9001 ataatacgcg ataattttat cttccggcgt gcgatggaaa aagaaggcga gattgatcgg
     9061 caacgcaagc gattcccatt gctcatccgc gaggtggaaa tccggcaacg catgaggctg
     9121 gcgcggtatt aatttaaatc ttccgccaat gactccttca aaccgcatcg cgcaagcgtc
     9181 gcacgcgcaa atcacctttc ggctggacct ctccaacaga tggcggtgtt cgggcccgac
     9241 cgccatgttg caaagttcgc aatgctcccg cgctgcgcga ggccgggcca ggcggcgcaa
     9301 agcggcgaat ggattgcctt gtccggtgga aagcttggga ttcatggtgg agcggtccct
     9361 gttcaagccg gcggaatttc cggcatcgaa cggtcgatcg gctcgcgatt ggcggggttg
     9421 atcccggtgg aatcgcgcgc ggagcgataa tacttccgat gaggattgcg gcccggctcc
     9481 ctttgtttaa gcgctttttc ttcgccgcgt ggagtgccgg ccacgtgaat tccggcggga
     9541 ggtctgttga tggaagtttg catgatattt tgtatagcgt atgaaaaaat gatgccttgt
     9601 tgccaccgct tcaccacgga catcgtccca atgaacagcc aaagcggtca catagtagat
     9661 tatcattttt cagaaacgct taaatagggt gaacacctca cgaacgaatt gtggcagaac
     9721 gaaaaaaagg cgcgcggatt gttccaaata accggcttgg atggaagcca atcagccgcg
     9781 gtccgtccct gcttgtgcag aaaaacaccc ctttttgcgt gtagggaaca aagtttttgt
     9841 cctacacgcc aaaaagccgt gttttttgaa aagcgggaaa cccgcataaa cattgatcaa
     9901 aatggctgaa aactcacgtt ttcagctaca tcaaaaaaaa tgctctcata cctaccgtag
     9961 gtttcatttt ttcggggagg ccatggtaat ctggtcacat gaacgatgaa aatcgagcat
    10021 tgggatggcg gggtgaggca gaagtgaagg gatggattgc gattgttcgg tgtccaccct
    10081 tcagggtgcg cgccagcgaa acgaagggga catgtgctgt tcggtgtcca ccctttaggg
    10141 tgtctgcgac cgcgcaaact ggaaaaaaca tgctaaagca tggacaccaa acagccttcg
    10201 aagcgccgag cgtttcagct aatccgattg ttcggtgtcc acgcttta
//
LOCUS       DASZMM010000362         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3704610, whole genome shotgun sequence.
ACCESSION   DASZMM010000362 DASZMM010000000
VERSION     DASZMM010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3704610"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VG754_08815"
     CDS             <1..1063
                     /locus_tag="VG754_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWF19357.1"
                     /translation="GTNLTYPLNRYTRFHQTSGTTGMPLRWLDTPESWDWMLESWGEV
                     YRAAGVNHNDRIYFAFTFGPFIGFWMAFEAGARMGALCIPGGGLSSAARVKAILDNGA
                     TVLCCTPTYALHLAEVAGEEKLDLQKSRVKAIMVAGEPGGSIPAIRTRLEQLWPGARV
                     YDHHGMTETGPVTFQCSKRTGVLHVIESAYYTEIIDPSTGKPVPAGEPGELVLTTLGR
                     IGSPLLRYRTGDLVKADTQPCACGRVELALVGGILGRTDDMVVVRGVNMYPSAVEEII
                     RAHDGVLEYQVQVNSVNSLTEISVRIEPQPGCADADALAARLEKSFQTQFALRVPVTV
                     APSGSLPRFEMKAKRWVKI"
     gene            1183..>2637
                     /locus_tag="VG754_08820"
     CDS             1183..>2637
                     /locus_tag="VG754_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWF19358.1"
                     /translation="MRTGALTNFAAMGVLAVIGYVDIANGSPSGEHHPAKEDTYTITS
                     VIQVLKPANPADMVDDFQEARVLAQDDDSYTVEITYFPLYQPEIGENRDWRKEDASMT
                     NYLHPTVTENWDEAMRRDLVAELHQAGIEVDKLTDKQLVEQVSRWAMRRARSTDAFAI
                     WAISYPDGKPAVFPALREAFDRQKPDAAWTDERMFEQEALGKSMFFQKVHGSCTSSSV
                     YMATILRALGIPTRIVFCIPPFDPNDDAQASMFYANIHHNKARETVRAALDGTGGFEN
                     HLFNEVYVGHRWVRLNYSTLGQPILDAHYFGLLTHIYTCSDLSRVPLAQTWGMRFFKY
                     PADQPRLSSVNPYRLISVQDHFGTNAHLDNPPVPMAELQTVTIIGLYLPNSPEVPRWV
                     AEGLSEHKINCDFLIASKEWLSGSHNQMRAFQKRVGHDFILTAPQHPDVKARIIDMRL
                     SSGDGSFQAYAAQVLPEDKPKLVSGISYNIQPLNV"
BASE COUNT          607 a          716 c          723 g          591 t
ORIGIN      
        1 cggcacgaac ctcacttacc cgctcaaccg ctacacgcgc ttccaccaaa ccagcggcac
       61 gaccggcatg ccgttgcgct ggctcgacac accggaaagc tgggactgga tgctcgaaag
      121 ctggggcgag gtctatcgcg ccgctggcgt gaatcataac gaccggattt attttgcgtt
      181 cacgttcggc ccgttcatcg gtttttggat ggcatttgaa gcgggcgcga ggatgggcgc
      241 gctctgtatt cccggcggcg gattgagcag cgccgcgcgc gtgaaggcga ttctcgacaa
      301 cggcgcgacg gtgctttgtt gcacgccgac ttacgcgctt catctcgccg aagtggctgg
      361 agaggaaaag ctggaccttc agaaatcgcg tgtgaaagcc atcatggtcg cgggggagcc
      421 gggcggcagc attcccgcaa tccgaacgcg gttggaacaa ctctggcccg gcgcccgcgt
      481 gtacgatcat cacggcatga cagaaaccgg cccggtgacc tttcagtgct ccaaacgcac
      541 cggcgtcttg cacgtgattg aatcggctta ttatacggaa atcattgatc catccaccgg
      601 gaagccggtt ccagcagggg aaccgggtga attggtgttg accacccttg ggcgcatcgg
      661 ctcgccattg ctgcgctatc ggacaggcga tttggtcaaa gccgacacgc agccttgcgc
      721 ttgcggccgc gttgaacttg cattggtggg cggaattttg ggacgcaccg acgacatggt
      781 cgtagtgcgc ggcgtgaata tgtatcccag cgcggtcgag gaaatcatcc gggcgcatga
      841 tggcgtgctg gaataccagg tgcaggtcaa ctccgtgaat tccctcaccg aaataagcgt
      901 tcggatcgaa ccacaaccag gatgcgcgga cgcggacgct ttggccgcgc gcctggaaaa
      961 aagttttcaa actcaatttg cattgcgtgt gccggttacg gttgcgccat ctggaagcct
     1021 gccgcgtttt gagatgaaag cgaaacgctg ggtaaaaatc taaaataaat ttatcaaaac
     1081 agcgattgga atggcacttg acgacgtaac attcattata tttcctcaaa tttaacaatt
     1141 cggccgcaac tgctgatggt aattggtgtt cattaaaaaa acatgaggac aggggcattg
     1201 accaactttg cggcaatggg cgtcttggca gttatcgggt atgttgacat tgccaacgga
     1261 agtccgtctg gcgagcatca cccggccaaa gaggacactt acacgataac ttccgtcatt
     1321 caggtcctta aaccagccaa tcctgccgac atggttgatg attttcagga agcccgcgtc
     1381 ctcgcgcagg atgatgattc ctacactgtc gagataacct atttcccgct ttatcaaccg
     1441 gagattggcg aaaatcgtga ctggcgcaaa gaagacgcct cgatgacgaa ctatcttcat
     1501 cccacagtca ccgaaaactg ggacgaagca atgcgccgcg atctcgtcgc ggaacttcac
     1561 caggctggca tcgaagtgga caagctgacg gacaaacaac ttgtcgaaca agtttcgcgt
     1621 tgggcaatgc gacgggcgcg ttcgaccgat gcgtttgcga tttgggcgat ttcttatccc
     1681 gacggcaaac cggcggtttt tcccgctttg cgcgaggcat tcgaccggca gaagccggat
     1741 gcggcttgga cggatgagcg catgttcgaa caggaagccc ttggcaagtc gatgtttttc
     1801 cagaaagtgc atggctcctg cacgtcctcc tcggtatata tggcgacaat tctccgcgcg
     1861 ttgggcattc caacgcgaat cgtgttttgc attccaccgt ttgacccgaa cgacgacgcc
     1921 caagccagta tgttttatgc gaacatccat cataataagg cgcgtgaaac agttcgcgcc
     1981 gcgctcgacg gcaccggcgg tttcgaaaac catctgttca acgaagtgta tgtggggcat
     2041 cgatgggtgc gcctaaacta cagcacgctg ggccagccga ttctcgacgc ccattatttt
     2101 ggcctattga cccacatcta tacctgctcg gacttgagcc gggtgccgct ggcgcaaacg
     2161 tgggggatgc gtttcttcaa atatccagcg gaccagccaa gactttcctc tgtgaatcct
     2221 tatcgtctca tctccgtgca agatcatttt ggtaccaacg ctcacttgga caatccgcct
     2281 gttccgatgg ccgaattgca aacagtcaca atcattgggc tttatcttcc gaattcccca
     2341 gaagtgccga gatgggttgc agaaggatta tcggaacaca aaatcaattg cgattttctc
     2401 atcgcctcca aggaatggct gtcgggaagc cataatcaaa tgcgcgcatt ccagaagcgc
     2461 gttgggcatg atttcatctt gaccgcaccg caacatcctg acgtcaaagc gcgcattatt
     2521 gacatgcgcc tcagttctgg tgacgggagc tttcaggcat acgctgcgca agtgttgccg
     2581 gaagataagc cgaagttggt ttctggaatt tcatacaaca ttcaacctct caacgtc
//
LOCUS       DASZMM010000363         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3450178, whole genome shotgun sequence.
ACCESSION   DASZMM010000363 DASZMM010000000
VERSION     DASZMM010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3450178"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VG754_08825"
     CDS             <1..752
                     /locus_tag="VG754_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWF19359.1"
                     /translation="VGIPWLGWTDGTCRFCRSDRENLCDNARFTGYTMDGGYAEYTVA
                     DEHFCFPIPPAYTDIEAAPLLCAGMIGYRSLTKTGDAKRIGFYGFGSAAHLLTQVARF
                     QHRQIYAFTRAGDLKGQESARTLGATWAGGSDTLPPEPLDAAIIFASVGELVPQALRA
                     LDKGGVVVCGGIHMSDIPSFPYEWLWEERSVCSVANLTRRDGEEFLSLAQKVPVVTNV
                     QPFPLAEANEAIDCFRHGKIEGSAVLVVDGK"
     gene            834..1157
                     /locus_tag="VG754_08830"
     CDS             834..1157
                     /locus_tag="VG754_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19360.1"
                     /translation="MPSETFQASRWTKGNVLFPTVIEVTDKAVVRRKRNWFSMDEMSM
                     SIFKVASVHIKTGIIWSNILIESSGGTDPIQSHGHSKADAQRIKELVEAYQADPQNRR
                     DAQNS"
     gene            1170..2333
                     /locus_tag="VG754_08835"
     CDS             1170..2333
                     /locus_tag="VG754_08835"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF19361.1"
                     /translation="MKRDVIVIGGGAAGLMCAIEAGRRGRSVTVLEHAERLGKKILIS
                     GGGRCNFTNVNAVPENYLSANPHFCKSALARYQPADFIALVEKHGIAYHEKKLGQHFC
                     DITSRQIVEMLQKECEAARVEIRLNSQVQQVQKNLCFAVQTNGETWECDSLLIATGGL
                     SFPKIGATDFGYRIAKQFGLKISGTRPGLVPLLFNEADRAAFGDLSGISVDAIVFSNG
                     ASFRENILFTHRGLSGPAILQISNYCGDGPITLDLLPNNPGSDFLKTQRETNKELKTF
                     LSQWLPQRFAQQWCESFAPSRPMNRYSNQELEQIARQLHQWEIHPAGDEGYAKAEVTL
                     GGVDTGELSSKTMESRRVPGLYFIGEVVDVTGWLGGYNFQWAWASGHAAGQVV"
BASE COUNT          560 a          684 c          684 g          556 t
ORIGIN      
        1 gcgtcggcat tccgtggctc ggctggacgg atggcacctg ccggttttgc agatcggacc
       61 gtgaaaactt gtgcgacaac gcgcgtttca ccggttacac gatggatggc ggttacgcgg
      121 aatacacggt tgccgacgaa catttttgtt ttcccattcc gccagcttac accgatattg
      181 aagccgcgcc gttgttatgc gcgggaatga tcggctatcg ctcgctgaca aaaaccggcg
      241 acgcaaaacg aatcggcttt tatggttttg gctcggcggc gcacctcctc acgcaggtcg
      301 cgcgatttca acaccgccag atttatgcgt tcacgcgcgc gggtgatttg aaaggccagg
      361 aatctgcccg caccctcggc gcaacatggg ccggcggttc cgataccttg ccgcccgaac
      421 cgctcgatgc cgccatcatc ttcgcgtcag ttggcgaact cgtgccacaa gcgttgcgcg
      481 cgcttgataa aggcggcgtg gtggtttgcg gcgggatcca catgagcgac attccgtcgt
      541 ttccttacga atggctttgg gaagaacgca gcgtctgttc cgtggcgaac ctcacgcggc
      601 gcgatggcga agaatttctt tcactcgcgc aaaaagttcc ggtggtcacg aatgtccagc
      661 cgtttccgtt ggctgaggcg aatgaagcga ttgattgttt ccgccacgga aaaattgaag
      721 gttcagccgt gctggttgtg gatgggaaat aattctgcga ccgttcaccc cattgcaaat
      781 cttgtttcgg cattgcatct ttttatgagc attgagacaa agagggaatc attatgccat
      841 ctgaaacatt tcaagccagc cgctggacaa agggcaacgt gctttttccc accgtcatcg
      901 aagtcaccga caaggcggtc gtccgccgca aacgcaactg gttcagcatg gatgagatga
      961 gcatgagcat tttcaaagtc gcttccgtcc acatcaaaac gggaatcatt tggtctaata
     1021 tcctcatcga atcctccggt ggcaccgacc cgattcaaag ccacggccac agcaaggccg
     1081 acgcgcaacg catcaaagaa ttggttgaag cttatcaggc cgacccgcaa aatcgccgcg
     1141 atgcgcaaaa tagttgagaa cgcgccagca tgaagcgcga tgtcatcgtc atcggcggcg
     1201 gcgcggcggg gttgatgtgc gcgattgagg ccggacggcg cgggcgttcg gtcacggtgc
     1261 tcgaacacgc ggaacgtctc ggcaaaaaaa tcctcatctc cggcggcgga cgctgcaatt
     1321 tcacaaacgt caacgccgtc ccggaaaatt atctttccgc caacccgcac ttctgcaaat
     1381 cggccttggc ccgttatcag cccgctgatt tcattgcgct cgtggagaaa cacggcatcg
     1441 cttatcacga aaaaaaactc ggccaacatt tttgcgacat cacctcgcgc cagatcgtcg
     1501 agatgctgca aaaggaatgc gaagctgcgc gcgtggaaat ccgtttaaat tctcaggtcc
     1561 aacaggtgca aaagaatctt tgctttgccg tccaaacaaa cggcgaaact tgggaatgcg
     1621 attcgctcct gatcgcgacc ggcggacttt catttccgaa aatcggcgcg accgatttcg
     1681 gctatcgcat cgcaaaacag ttcggcctga agatttccgg cacgcgcccg ggattggttc
     1741 cattgctgtt taatgaagcg gaccgcgcag ccttcggaga tttgagcggc atttcagtcg
     1801 atgccatcgt cttcagcaat ggcgcgagtt ttcgcgaaaa cattttgttc acgcatcgcg
     1861 gcttgagcgg cccggcaatc ctgcagattt ccaattattg cggtgacggg ccgatcactt
     1921 tagatttgct gccgaacaat cctggcagcg acttccttaa aacgcagcgg gaaaccaaca
     1981 aggaattaaa aacgtttttg agccaatggt tgccgcaacg gttcgcgcag caatggtgcg
     2041 agtcattcgc gccgtcgcgc ccgatgaacc gctattcgaa tcaggagctg gaacaaatcg
     2101 cgcgacagtt gcatcaatgg gaaatccatc ctgccggcga cgaaggctac gcaaaggccg
     2161 aggtcacgct cggcggtgtg gacacaggcg aactgtcatc aaagaccatg gaatcgcggc
     2221 gcgtgcccgg tctttatttc atcggcgaag tcgtggacgt gaccggttgg ctcggcggct
     2281 acaattttca gtgggcctgg gcatcgggcc acgcggcggg acaagtggtg tagaagcttt
     2341 cgccgtctaa tgtttttttg gatcagcagg tttctccttg ctccatctca ttgcttcata
     2401 acgcaaaccg ttatggtaat ccgggactat cttcagttca gccctctgca acaggttgtt
     2461 ttcgtcgaac tgaaggaaag cgac
//
LOCUS       DASZMM010000364         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2896503, whole genome shotgun sequence.
ACCESSION   DASZMM010000364 DASZMM010000000
VERSION     DASZMM010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896503"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VG754_08840"
     CDS             <1..393
                     /locus_tag="VG754_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWF19362.1"
                     /translation="RVQAATGLAVKKAVELNLEADFDGELQVDAALVPEIAARKLPES
                     KVAGSATVLIFPDLNSGNIASKLIQHVARANAYGQILLGLDRPAADVSRGSNAHDILG
                     VAAIVGLQSIEYAKLYPGAGSTVPGEGS"
     gene            430..1524
                     /locus_tag="VG754_08845"
     CDS             430..1524
                     /locus_tag="VG754_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF19363.1"
                     /translation="MNTETPRVFIAATRQNDGKTTTSLGLISALQQYYPRIGYIKPVG
                     QRFVEIEEQKIDEDTVLMDSVFRLNCPLVDMSPIAVEPDFTRKYLQSSNNDALVKKIQ
                     KAFDRVAWEKDFVLCEGSGHAGVGSVFDLSNAQVAKVVGAKVIIVTQGGIGRPIDEVS
                     LNQALFEKEGVEIIGVILNKVLGTKVEYISDFARRGLKRKGLELLGVIPHHQVLSNPT
                     VDLIREELRAEMLNQSGGLQNLVEDVAVGAMSAQNAQSLFKPGVLLIAPGDREDIILA
                     AHKSLEGQKGRMMAGVVLTGNLRPTNGALKIVRSFPIPVLLAEDDSYQVASKVHDLTV
                     KTRPGDEQKIKLIRDLIAKNVDVQKILNSL"
     gene            1526..2626
                     /gene="hisC"
                     /locus_tag="VG754_08850"
     CDS             1526..2626
                     /gene="hisC"
                     /locus_tag="VG754_08850"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWF19364.1"
                     /translation="MSTSLPLNPALEQVPVYQPGRPIEEVARELGLPAHDIIKLASNE
                     NPLGPSPAALAAMQKVIASLHLYPDGNAFYLKQKLAQKLGLTTSNLILGNGSNEIIEF
                     VGHALMGPGVDVVMSQYCFAVYPIVTRLFGANPIVVPARNYGHDLPAMLKAITPRTKA
                     IFIANPNNPTGTLASREDILRLVNEVPKQVLLVMDEAYIEFLDEPVDLLPSIRSGNHP
                     NLLLMRTFSKIFGLAGLRLGYGIAQPELIAALEKIRQPFNINSIAQAGALAALDDVEH
                     VRKTRENNAEGLRFFSEHLREMGLEFIPSAANFILVRVSNGQQIFESLQKQGVIVRPL
                     GGYQLPDWIRISIGTPKENNRCLGALKQALAS"
BASE COUNT          642 a          759 c          775 g          607 t
ORIGIN      
        1 cgtgttcaag ccgcaaccgg ccttgccgtc aagaaagccg ttgaactgaa tttggaagcc
       61 gattttgacg gtgaattgca ggtggacgcg gctttggtcc cggaaattgc cgcgcgcaag
      121 ttgcccgaaa gcaaggttgc gggcagcgcg acggtgctga tttttcctga tttgaactcg
      181 ggcaatattg cgagcaaatt aatccaacat gtcgcgcgcg cgaatgcata cggccagatt
      241 ttgctcggct tggaccggcc cgctgcggac gtttcgcgcg gttccaatgc gcacgacatc
      301 ctgggcgtcg cagcgatcgt cgggttgcaa tccatcgaat acgccaaact ttacccgggc
      361 gcgggctcaa ctgttccggg cgaaggttcg tgaaattaga agcagagaag ttttgtgcaa
      421 cgcctcctca tgaacacaga gactccgcgg gtcttcatcg ccgccacccg gcaaaacgat
      481 ggcaaaacga ccacgtcgct cgggctgatt tccgccctcc aacaatatta tccccgcatc
      541 ggttacatca agccggtcgg ccagcggttt gttgagatcg aggagcaaaa aattgacgag
      601 gacacggttc tcatggacag cgtgtttcgg ctgaattgtc cgttggtgga catgagtccg
      661 atcgccgtcg agcctgattt cacgcgcaaa tatcttcaat cctcgaacaa cgacgcgctg
      721 gtgaaaaaaa tccagaaagc ttttgaccgc gtggcgtggg aaaaagattt tgtgctgtgc
      781 gaagggtccg ggcacgcggg cgtgggttcg gtgttcgatc tgtcgaatgc gcaggtggcg
      841 aaagtggtcg gcgcgaaagt catcatcgta acgcaggggg gcatcggccg accgattgac
      901 gaagtttcgt tgaaccaggc tttgttcgag aaggagggcg ttgagatcat cggggttatt
      961 ttgaacaagg tcctcgggac gaaagtggaa tatatcagcg attttgcccg gcgcggtctc
     1021 aagcgcaagg gcctcgaatt gcttggcgtc attccgcacc atcaagtgct gtcgaatccg
     1081 accgttgatt tgatccgcga ggagttgcgc gccgaaatgt tgaaccaaag cggcggtttg
     1141 caaaatttgg tggaggacgt tgccgtcggc gcgatgagcg cgcaaaacgc gcaaagtctt
     1201 ttcaaacccg gcgtgctcct catcgcaccc ggtgaccgcg aggacatcat ccttgccgcg
     1261 cacaaaagcc tcgaaggcca aaaaggcaga atgatggcgg gcgtcgtcct gaccggcaac
     1321 ttgcgcccaa ccaatggcgc gcttaaaatc gttcgcagct ttcccatccc ggtgttgctc
     1381 gccgaagacg acagttacca ggtcgcttcc aaggtccacg atctcacggt caagacgcgc
     1441 cccggcgacg aacaaaaaat caagctcatc cgcgatttga tcgcaaaaaa tgtggacgtc
     1501 caaaaaatct tgaactcgct ttgatatgtc cacgtctctg cctctgaatc ccgccttgga
     1561 acaggtgccg gtgtatcagc cgggccggcc gattgaagaa gtggcgcgtg aactcggcct
     1621 gccggcgcac gacatcatca agctcgcttc caacgagaat ccgctcggcc cgtcacccgc
     1681 cgcactggcc gcgatgcaga aggtgatcgc cagcctgcat ctttatccgg acggcaacgc
     1741 gttttacctg aaacaaaaac tggcgcaaaa acttggcctc acaacgtcca atctaatcct
     1801 tggcaacggc tccaacgaaa tcatcgaatt cgtcgggcac gcattgatgg ggccgggcgt
     1861 ggatgtcgtg atgtcgcaat attgcttcgc ggtttacccc atcgtgacgc ggttgttcgg
     1921 cgcaaatccg attgtcgttc ctgcgcgaaa ctacgggcat gatttgcccg ccatgctcaa
     1981 ggccatcacg ccacgcacaa aagcaatttt cattgccaat cccaacaacc cgaccggcac
     2041 tctggcttcg cgcgaagata ttctgcgcct cgtcaacgaa gtgccgaagc aggtgctgtt
     2101 ggtcatggac gaggcttaca tcgaatttct tgatgagccg gtggatttgc tgccgtcgat
     2161 tcgcagcgga aatcatccga accttttgct gatgcggaca ttttcaaaaa ttttcggtct
     2221 tgcgggtctg cgccttggtt acggcatcgc gcagccagaa ttgattgccg cactcgaaaa
     2281 aatccgccag ccgttcaaca tcaattccat tgcccaagcc ggcgcgttgg cagcgctgga
     2341 cgatgttgaa cacgttcgca aaacgcgcga gaacaatgcc gagggactgc gatttttcag
     2401 cgagcacctg cgcgaaatgg gattggaatt catcccgtca gcggcgaatt tcattcttgt
     2461 ccgcgtcagc aatggccagc aaatcttcga aagcttgcaa aagcagggcg tcattgtgcg
     2521 tccgctgggc ggctatcaat tgccggactg gatacgcatt tcgattggaa cgcccaagga
     2581 aaacaatcga tgcctcggag cgctgaaaca ggcccttgct tcatgattgg cggttgccga
     2641 taatgcaggt gttgagaaaa gattttgttg tgattcacgg cttttaatac ctccaataaa
     2701 agacgtgcat tttcccggcg ttgaactaaa attgacacga tcatgtatcc accagtggtc
     2761 acacgagacc cgacggctgt tga
//
LOCUS       DASZMM010000365         4741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_141183, whole genome shotgun sequence.
ACCESSION   DASZMM010000365 DASZMM010000000
VERSION     DASZMM010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141183"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VG754_08855"
     CDS             <1..256
                     /locus_tag="VG754_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWF19365.1"
                     /translation="PGVPANLEIISGFAKDVPYLQREFWKQYPKARATDFAKFLAMAK
                     RGKAIDGHHSPDSKAEQDYQKSELERSLKYCKEVLGLGLK"
     gene            392..1609
                     /locus_tag="VG754_08860"
     CDS             392..1609
                     /locus_tag="VG754_08860"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF19366.1"
                     /translation="MILNIFSDVKFFGGGRTSVVPARIIAVFFTVFLLLTFITQSHAQ
                     PNGTYNVTEQWVVTIQEGPTPPVRYQGRESGTLVINNGQYSLVDHTGITNGDPYNATV
                     HSIAGGPPYTSIGEYPYAGWGIATNGLVNIMHLGFFSVGINRSSPSDGTLMTGKSSVF
                     SMTGQSLTGLSGSGTQDNGNEFYFTASSSSSLVQQGGVGPVIVSQPKNQTVPAGSNAM
                     FSVTATGSPPPTYQWRFNGTNIALATGTSYIVTNAQIANEGNYSVIVSNSIGTVTSTN
                     ATLTIVPASFFMGEVLLPPYTNGWYWMGPQGANTYGFGYFNTANFPYVLHNDMGWEYF
                     IDANNAGGGGYFYDFTDSAWLYTEPDLFPYIYDFNVNAWLFYLHQGNAPDQYTSNPRW
                     FLNTSTQTWGNHL"
     gene            1815..3983
                     /locus_tag="VG754_08865"
     CDS             1815..3983
                     /locus_tag="VG754_08865"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF19367.1"
                     /translation="MLRAAVSFTVTPTNIDSASVGTVTLQITGVSAGANVLIQKFVDY
                     NGNGAVDAGEPMIQSFQVTDNHAFVFNGVTNTAVPGDSNPTSGAITVVLNAINNGIVG
                     GTSGKFVFKLSSPGGVFSPLYKAFTVTATTNSGYGTITGTVTYNGSAIANPAVVVFTP
                     STINGNTSAGGTFANSSGSYTVKVVPGSYVVGALKSNYVSNVGSSAHIVTVTAGATVT
                     NNLTVVAATETISGKFYDTNTSVGLAGFLMPAQTTNGNLAVGFTDVNGNFKIPVISGQ
                     WKISSNERTLGMLGYLRPQNSTKVNATNGSVSGVNIAVTKATAMIYGRFVDNLGNPVP
                     NVDLYADDGQSQYESEAITDGNGYYFAGANSSASANWNVGLNTPLDPALQYYDFTDSY
                     NGANLISGEALSEDFGAVAAPYKITGSVLNASNAPIVGVQVYASATINGTNYQAVNGT
                     TDASGQYVLYVAGNSTWYVNVDCQNNNNNLNNQGYNCVDTEIVSIVNNNGIANFVAPA
                     FGVQELDGSLTDNNGNPISGITVYAVSEDSYPSQSTTTDANGFYEFVVGNGTWDVSVD
                     CTNLAALNYDCPGEQDGNLVAAANVSGVDFFAQSSIPPFFTGEVSLGNGGWYWLGPQG
                     SDTYGFGYFNTANFPYILHLDMGWEYFLDAGDAGSGGYFYDFTDGVWFYTTPGLFPYI
                     YDFNLNVWLFYSPQTNATDRYTSGPRWFLNTSTQNWINDL"
     gene            4226..>4741
                     /locus_tag="VG754_08870"
     CDS             4226..>4741
                     /locus_tag="VG754_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF19368.1"
                     /translation="MIEVKNLSKAFGPKLAVNNVSFTVKRGEVLGFLGPNGAGKSTTM
                     RMLTGFIPPSEGKISVGGFDMLTNPIAAKRLIGYLPESAPAYIDMTVQGFLSFCAELS
                     GLRKDERKKAVHRVAEMCFLESVLHQSVETLSKGYRHRTCFAQSIIHDPDVLVLDEPT
                     DGLDPNQKHEIR"
BASE COUNT         1112 a         1298 c         1205 g         1126 t
ORIGIN      
        1 tcccggcgtg ccggccaatc tcgaaatcat ttctggattt gccaaggacg ttccttatct
       61 ccagcgcgaa ttctggaaac aatatcccaa ggcgcgcgcg acggattttg cgaaatttct
      121 cgccatggcc aagcgcggca aggccatcga cggccatcat tcacccgatt ccaaggccga
      181 gcaggattat caaaagagcg agttggaacg cagcctcaaa tattgcaagg aagtgctggg
      241 actgggattg aagtgattcc cggcctgcac cattcgattt gccatccgag aaaagcatcc
      301 gattggcagg attatccttg acgttttccc gcgggatttg gaatatggtg ccacgctcgt
      361 caactgcatt ccgcaatctg acggagtgcg catgattttg aatattttta gtgacgtaaa
      421 gttttttggg ggtggtagaa ccagtgttgt acccgcgcga atcatcgcag ttttttttac
      481 ggtttttctg ttgctgacgt tcatcacaca gtcgcatgcc caaccgaatg gcacttacaa
      541 cgtcacagag caatgggtcg tgacgatcca ggaaggcccg actccgccgg tgcgttatca
      601 ggggcgggaa agcggaactc tcgtgatcaa caacggccag tattcgcttg ttgatcatac
      661 cggcatcacc aacggcgacc catacaacgc gacggtccac agcatcgcgg gcggtcctcc
      721 gtacaccagc atcggcgaat atccttatgc gggatggggc atcgcgacca acggattggt
      781 gaacatcatg caccttggtt tcttttccgt gggaattaat cgttcgtcgc caagcgacgg
      841 gacgctgatg acgggcaagt cctctgtttt ttcaatgacc ggccaatcgt tgaccggtct
      901 ttcaggttca ggcacacagg acaacggaaa tgagttttat ttcaccgcat cctcttcaag
      961 cagtctcgtc caacagggag gtgttggtcc ggtcatcgtt agccagccga aaaaccaaac
     1021 cgtcccggca ggcagcaatg caatgttcag cgtgaccgcg accggttcgc cgccgccgac
     1081 gtatcaatgg cgattcaatg ggacaaatat cgcccttgct acaggcacaa gctacatcgt
     1141 cacgaatgca caaatagcaa acgagggcaa ttattccgtg atcgtctcca acagcatcgg
     1201 cactgttacg agcacgaatg ccacgcttac aatagtgcca gcgtcattct tcatgggcga
     1261 agttttattg ccaccctata ccaatggttg gtattggatg ggacctcaag gcgccaacac
     1321 gtatgggttt ggttatttca atacagcgaa cttcccctac gttttgcaca acgacatggg
     1381 ctgggaatat ttcatagatg ccaacaacgc ggggggcggc gggtatttct acgatttcac
     1441 ggacagcgcc tggctttata ccgagcccga tctgtttcct tacatctatg atttcaacgt
     1501 gaacgcatgg ctcttttatc ttcaccaagg caacgctccg gaccaataca cctccaatcc
     1561 gcgctggttt cttaacactt cgacccagac atggggcaac catctttagt cttgttcaca
     1621 aaaaaggccg cctttttgac ttcattattt aaatttgaga attgctattg acttgttcaa
     1681 aggttttcca atatagaagt gcccaagaag acgcctgacc aactcctgaa cctaatatga
     1741 aagaccacat taagtccttt tgtcgttctg tcctgtttct gacagtcata tctttggccc
     1801 tgtttgcggg cccagtgctg cgcgcagccg tcagttttac cgtcactccc accaatattg
     1861 acagcgcatc cgtggggacg gtgactctcc aaatcaccgg ggtgagtgcc ggcgcgaacg
     1921 tcctcatcca aaaatttgtt gactacaacg gcaacggagc ggtcgatgct ggcgagccaa
     1981 tgatccagag ttttcaagtc accgacaacc acgcgtttgt atttaacggg gtgacgaaca
     2041 ccgccgtgcc cggcgattcc aacccgacga gcggcgcgat caccgtggtg ttgaacgcta
     2101 ttaataacgg gattgtgggc ggcaccagtg ggaaatttgt attcaagctg tctagtccgg
     2161 gcggcgtgtt ttcgccgctt tataaggcat ttacggtgac cgccaccacc aacagcgggt
     2221 atggcaccat cacgggcaca gtgacctaca acggttccgc catcgcaaat cccgctgtcg
     2281 ttgtcttcac cccctccaca atcaacggca acacttccgc tggcggcact tttgccaaca
     2341 gttcgggcag ctataccgta aaggttgtgc cgggaagtta cgtggtggga gcgctcaaga
     2401 gcaattatgt gtccaatgtg ggcagcagcg cccatattgt cacggtcacg gcaggcgcga
     2461 cagtcaccaa caatttgacg gtcgtggcgg cgaccgagac catatccggc aagttttatg
     2521 ataccaacac gagcgtcggc ctcgccgggt ttcttatgcc ggcccaaacc acgaacggaa
     2581 atctggccgt tggctttaca gacgtgaatg gcaatttcaa gatccccgtc atcagcgggc
     2641 aatggaaaat ttcgagcaat gagcgtacct tgggcatgct tggatatttg aggccgcaaa
     2701 acagcacgaa ggtgaacgcc accaacggca gcgtttcggg cgtgaacata gcggtgacca
     2761 aggcgaccgc gatgatttat ggccgtttcg ttgacaatct cggcaatcct gtcccgaacg
     2821 tggatttgta tgccgatgac ggccagagcc agtacgaatc cgaagcgatc acggatggca
     2881 atggatatta tttcgcaggc gcaaattcgt ccgcgtctgc caattggaat gtcggcctca
     2941 acacgccatt ggacccggcg ttgcagtatt atgatttcac cgacagctac aatggcgcca
     3001 atctcatctc tggcgaggcc ctgtccgagg attttggggc agttgccgca ccctataaaa
     3061 tcaccggttc cgtgctcaac gcgagcaacg cacccattgt cggcgtgcaa gtttacgcga
     3121 gcgcaaccat caatggcaca aactatcaag cggtcaatgg caccaccgac gccagcggcc
     3181 aatatgttct gtatgttgcc ggcaactcga cctggtatgt gaacgtggat tgccagaaca
     3241 ataataataa tctcaacaac caaggatata attgcgttga cactgaaatc gtcagcatcg
     3301 tcaacaataa tggcattgcg aatttcgtgg ctcccgcatt tggcgtccag gaactcgatg
     3361 gaagtttgac cgacaacaat gggaacccga tttctggcat cacagtctat gccgtcagcg
     3421 aagactctta tccgagccag tccaccacca ccgatgccaa tgggttctac gaatttgtag
     3481 tcggcaatgg aacttgggac gtgagcgtgg attgcacaaa ccttgccgct ttgaattacg
     3541 attgccctgg cgagcaggac ggcaatcttg ttgctgccgc caacgtgagc ggcgttgatt
     3601 tctttgccca gtccagcatt ccgccgttct tcacgggcga ggtttcgttg ggcaatggcg
     3661 gatggtattg gttgggacct caaggttccg acacttatgg ttttggttat ttcaatacag
     3721 cgaatttccc atacatcctg cacctggaca tgggctggga atattttctc gatgctggcg
     3781 atgcgggaag cggcggctac ttctacgact ttacagacgg cgtttggttc tacacgacgc
     3841 ccggcctgtt tccttacatc tacgatttca acctgaacgt gtggcttttt tatagtcctc
     3901 aaaccaacgc gactgaccgc tacacctccg gtccgcgctg gttcttgaac acttcaactc
     3961 agaattggat caacgacctc tgatttctac tttttgaccg ccctttgaag ctcggtgata
     4021 ttccaccgag cttcaaagct ggcgacaatt gccagtcgca aaacgcgata ccagattctt
     4081 tcgccttcaa gccgttcgca ggacttcggg cgcaaacaaa attcgcgtcc cgatgcattt
     4141 tatatttgaa cgggtgctcc gttgttgatt agtatttggc gcacacatcg cagccgattc
     4201 ccggtcgcat ttacgacgtt tcgcaatgat tgaagtcaaa aacctcagca aagccttcgg
     4261 cccgaaactg gccgtcaaca acgtttcctt caccgtgaaa cgaggcgagg tgctcggctt
     4321 tctcgggccg aacggcgcgg gaaaatccac caccatgcgg atgctcaccg gcttcatccc
     4381 gccgtccgaa ggcaagatca gcgtgggcgg cttcgacatg ctgacgaatc caatcgccgc
     4441 gaaacggctc atcggctatc tgcccgagag cgcgcctgca tatattgata tgacggtgca
     4501 aggttttctg agcttttgcg ccgaactcag cgggttgcgc aaagacgaac gaaagaaggc
     4561 cgtgcatcgc gtcgccgaaa tgtgttttct ggaatcggtc cttcatcaaa gcgtggagac
     4621 gctttccaag ggttatcgcc atcgcacctg cttcgcgcaa tccatcattc acgatcccga
     4681 tgttctcgtg ctggacgaac ccaccgacgg tttggacccg aatcaaaagc atgaaatccg
     4741 g
//
LOCUS       DASZMM010000366         8586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2067988, whole genome shotgun sequence.
ACCESSION   DASZMM010000366 DASZMM010000000
VERSION     DASZMM010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067988"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VG754_08875"
     CDS             <1..925
                     /locus_tag="VG754_08875"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HWF19369.1"
                     /translation="GESLTALLHQAAQVRWQAKAHHFAARARQAGWEQSLWEGLFRAL
                     GYKQNVWPMQRLAELRPHWHATSRDPLLYQARLFGISGLLPAELTRAQAATDDYLRRV
                     WDQWWREREQFADCILPRDIWRFNNLRPANHPQRRLALAAHWLAACDLPARLERWFTA
                     KIPDGELADSLLQTLQVESDEFWSWHWTFRSARLARSQPMLGAARVTDLAVNVILPWF
                     WMRAVEGKNETLQQVAEHRYLNWPVAEDNSILKLARQRLLGGAKPGILQGAAAQQGLL
                     QILRDFCDHSNALCENCEFPELVRNFASLRI"
     gene            963..1220
                     /locus_tag="VG754_08880"
     CDS             963..1220
                     /locus_tag="VG754_08880"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HWF19370.1"
                     /translation="MASITIKGMPKDIHAQLKREAEANFRSLNQEVLARVERTFEIDA
                     ALNTKRDQKWIDEALASGSVSPLTEKEMDAIRNRILKRKAA"
     gene            1217..1531
                     /locus_tag="VG754_08885"
     CDS             1217..1531
                     /locus_tag="VG754_08885"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF19371.1"
                     /translation="MRRVYKCPQFLLDLAEELTWLNEKAGAEVANAWYQSLKETIRPL
                     EKHPRLGRPRKDLSPTGIRSWRLARFPRWLLFYGIDEKEHLVLHRVRQGSMNLLVLKM
                     ES"
     gene            complement(1568..2272)
                     /gene="infC"
                     /locus_tag="VG754_08890"
     CDS             complement(1568..2272)
                     /gene="infC"
                     /locus_tag="VG754_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWF19372.1"
                     /translation="MSRPFSPRSSSPSASFVRVNGKIRAREVRVIGVDGTQLGVLSLP
                     EALTMARNNGVDLVEIAAAAVPPVCRLVDFGKFRYEQAKKDKESKKHQHANKVKEIQL
                     SPKIDPHDLGVKLTHAVDFLCEDMKVKVVLKFRGREMAHTEYGFQLVQKFLTDITPYG
                     HPDAPPKMLGRAINVMISPLPRNKRAKNPSEEHEQSEAPQAHKKNSNHHLENGVPHPD
                     HSEPHATDGAQASDAV"
     gene            complement(2595..3710)
                     /gene="recA"
                     /locus_tag="VG754_08895"
     CDS             complement(2595..3710)
                     /gene="recA"
                     /locus_tag="VG754_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWF19373.1"
                     /translation="MPPKTAEKPEKSAEKPSAKSAVAEPRAAGSENGRAVSPTRDRDL
                     EAAISSIHKAYGDGSIMRLGEARAKVGIQFIPTGALGLDLALGVGGLPRGRVVEIFGP
                     ESSGKTTLMLHVIANAQKGGGLAAFIDAEHALDPAYAKKLGVNLDDLLVSQPDSGEEA
                     LSICETLARSNALDVIVIDSVAALVPKAELEGEMGMATMGMQARLMSQALRKLTAILN
                     KSKTTCIFTNQLREKVGVMFGNPETTPGGKALKFYASVRLDIRRKETLKDASGNAIGN
                     HVKVKVVKNKVAPPFCETEFDIIYNHGINKEGSILDVGIEAGVVDKKGAWLQFEGDLI
                     GQGKDAAQKTLAEKPDLAKKIVEAILAKRNGTAVAAV"
     gene            complement(4510..6225)
                     /locus_tag="VG754_08900"
     CDS             complement(4510..6225)
                     /locus_tag="VG754_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF19374.1"
                     /translation="MASELIATEPDEDEQFYRETESIAFPKLNDRQLAMLEQLGSRRI
                     LKRGELVYKAGQRDLGLTVVLRGAVEVFDSLDGQEQILANAGPRTFVGDVAMLMGTAA
                     VASARGNADESEILEVPADRLRKALAEIPNVSEPIVRAFIMRRKRLQRDREFAGLRIV
                     ASDDSREGRRLDDFLDKNHVPHRLIDFQSEYGRALCDKLHLASHDLPALLTANGMPLR
                     HPSLREAARIAGLLRSAATDDEGEITCDLAIVGAGPAGLAAAVYAASEGLHTVVLESY
                     APGGQAGASSLIENFFGFPTGVRGGDLTYNAQLQAYRFGAKFATPAQALSLAFMDGEH
                     QALLQAEGCNATLRAKCVIIATGAEYRRLEADRREDFEGCGVYYAATGREAQLCRGST
                     VIVTGGGNSAGQAAMFLSEVAEKLLLVIRGDGLAKSMSVYLSRRVETKDNIEILSRTQ
                     IVRMFGDKALEAAELENTVTHERRTIQTPAIFSMIGANPCTSWLPPEIECDEKGFIKT
                     GHAVADCPAWKGMNRAPSPFETSRPGVFAAGDVRSGSVKRCAAAVGEGGAAVAGVHEV
                     LRTYA"
     gene            complement(6210..6563)
                     /locus_tag="VG754_08905"
     CDS             complement(6210..6563)
                     /locus_tag="VG754_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19375.1"
                     /translation="MGEGERQPTVTVHSQEQCQDAPKIGGRFLELLMIENFGCRNSGT
                     GKSPEPADKNVCAMWRRLSSLRVEGTFQSLVGNSSKMSPIDKGMNQVEDAFGVPTGTM
                     EEKTPFKLEIAWQAN"
     gene            6997..>8586
                     /locus_tag="VG754_08910"
     CDS             6997..>8586
                     /locus_tag="VG754_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19376.1"
                     /translation="MFPSVFCPALSLRAFFRKLALCLCLFALVVCGLTFSSFASTNMT
                     PITVTGWNRDVMIESNAVGPPFTAYASEVGPGDGNVFYQTGLPGYVWGLPPSGNFASM
                     IGDGTLFSFQPYTANNALVLSSETGLTSGTLTLVTPATYGSIAILSDSVNGTNQTGSL
                     TLNFQDGTSFTTTYFTPDWVGGTYGTAWFGNGKINLNNNGDSGGTQSPRYFQTTVNLT
                     ALLGATNKPLASITFGQSVSKATAIYCVSGVFAGNTLPVALPVSVTGFNRDIVVENTA
                     SGPPYTNYAVELNPAEGNAFYQSGLPGKSYGFPTNGMFSSAVDGTIFQLQSYTANNAL
                     VLSSDTGISQGTLTLTAPATYDSLSVIANSASGGGIPVVTINFSDGSSLVTNFNAPDW
                     FAGNQDIALAGFERINLTNGATQGAPANPRLYQTTYDLTALYGATNKTVKNLTFNQAS
                     GAKSTAVYAVSGVRGNQQPSATYQFAGVTNLPASAIQARSATLNGNIISLGGCIPEVF
                     AYYGTTDGHTNPTAWSQKMYLG"
BASE COUNT         1770 a         2467 c         2418 g         1931 t
ORIGIN      
        1 gggagaaagc ctcaccgcat tgctgcatca agccgcgcaa gtccgttggc aggcgaaggc
       61 gcatcatttc gcggcgcgag cgcgtcaagc gggatgggag cagtcgttgt gggaaggact
      121 ttttcgcgcg ctcggttaca agcagaacgt ctggccgatg caacgccttg ccgaattgcg
      181 tccgcattgg cacgccactt cgcgcgaccc gctgctgtat caagcgcggc tcttcggcat
      241 cagcggtttg ttgccggctg aattgacccg cgcccaagcc gcgacggacg attacctgcg
      301 ccgcgtctgg gaccaatggt ggcgggagcg ggagcaattt gcggactgca ttcttccgcg
      361 cgacatttgg cgcttcaaca atttgcgtcc ggcgaaccat ccgcaacgcc gccttgcgct
      421 cgcggcgcat tggctcgccg cgtgtgattt gccggcgcga ttggaacgct ggttcaccgc
      481 gaaaatcccg gatggcgaac tggcggattc gctcttgcaa acgctgcaag ttgaaagcga
      541 tgaattttgg tcgtggcatt ggacatttcg ttcggcgcgg ttggcgcgtt cgcaaccgat
      601 gcttggcgcg gcgcgtgtca ccgatttggc ggtgaatgtc atcctgccat ggttttggat
      661 gcgcgctgtg gaaggcaaaa acgaaacgct tcaacaagtc gccgaacatc gttacctgaa
      721 ctggcccgtc gccgaggaca acagcatttt gaaacttgcg cggcaacgat tattgggcgg
      781 cgcaaaacct ggcattctcc aaggcgcagc cgcgcagcaa ggtttgctgc aaatcctgcg
      841 cgacttttgc gatcactcga acgccctgtg cgaaaactgc gaatttccgg aattggtgcg
      901 gaattttgcc agtttacgga tttaaccttt acatccattg tggttgcgtt atggttgtca
      961 atgtggcgag cataacaatc aaaggcatgc ccaaggatat tcacgcgcaa ctcaagcggg
     1021 aggctgaagc gaattttcgc agcttgaacc aggaggttct ggcgcgggtg gagcggacgt
     1081 tcgaaataga cgcagccttg aataccaagc gcgaccaaaa atggattgat gaagcgttgg
     1141 ccagcggttc ggtgagtccg ctgactgaaa aggaaatgga tgcgatccgc aaccggatat
     1201 tgaagcgcaa agcggcatga ggcgggttta caagtgtcct caattcctgc ttgatctggc
     1261 ggaggaattg acctggttga atgaaaaggc cggggcagag gtggcaaatg catggtatca
     1321 atcgctcaaa gaaaccattc gtccgctgga aaaacacccg aggcttggac gcccaagaaa
     1381 ggatctttcc ccaacgggca ttcgatcctg gcgtttggct cgctttcctc gctggcttct
     1441 tttttacgga attgatgaga aggaacattt ggtcctccac cgtgtgcggc aagggagcat
     1501 gaatctcttg gtgttaaaaa tggaatctta gggcgcgcgc gccatcgcgt tcacaaactt
     1561 tcgcttttca gacagcgtcc gatgcctgcg cgccgtcggt ggcgtgcggc tcggaatggt
     1621 ccgggtgagg aacgccattc tccaaatggt gattggaatt cttcttgtgc gcttgcggcg
     1681 cttcggattg ctcgtgctct tcgctcgggt tcttggcgcg cttgttgcgg ggcaggggac
     1741 tgatcatgac gttgatggcg cggcccaaca tcttcggcgg ggcgtcggga tggccgtaag
     1801 gggtgatgtc ggtcagaaat ttttggacca actggaaccc gtactccgtg tgcgccattt
     1861 cgcggccgcg gaatttcagg acgaccttga ccttcatgtc ctcgcaaagg aaatcaacgg
     1921 catgggtcag cttcacgcca aggtcatgcg ggtcgatctt cgggctgagc tggatttcct
     1981 tgaccttgtt ggcgtgctga tgcttcttgg attccttgtc ctttttggcc tgctcgtagc
     2041 ggaacttgcc gaaatccacc aggcggcata ccggcgggac ggcggccgcg gcgatctcga
     2101 ccaggtccac gccattgttt cgcgccatgg tcaacgcctc gggcaacgac aggacgccca
     2161 gttgggtgcc atccacaccg atgacccgga cttcacgggc gcgaatcttc ccgtttaccc
     2221 ggacaaagga agcggaagga gaagaagaac gtggagaaaa ggggcggctc aagacaccct
     2281 cgtcggttta aacttgttca tgcaatcgta tataacactg tctgacaggc acaatgcccg
     2341 gctattctta gcaaagctaa ataagcgcat ctttaaatga aacacaagta aataattaac
     2401 ccacctttgt tacaaatagg tgcccattaa acgggttcaa attccaagca ccaaactcca
     2461 acatccagag aaactccaaa gatcaaacag cacgttcaca catgctgccg taagaattgg
     2521 agcttggaat ttgaagcttc actggtgctt ggtgcttgga cgttggtgct tcgatgggcg
     2581 tcgggtattt tatgtcaaac ggcggcaacg gctgtcccat tgcgcttggc taaaatggct
     2641 tcgacaatct ttttcgccaa gtcgggcttt tcggccaatg ttttttgcgc ggcatccttg
     2701 ccctggccga tgaggtcgcc ttcgaattgc agccatgcgc cttttttgtc cactacgccg
     2761 gcttcgatgc ccacgtcgag gatgctgcct tccttgttga tgccgtgatt gtaaatgatg
     2821 tcgaactccg tctcgcagaa cggcggcgcg actttgtttt tcaccacctt caccttcaca
     2881 tgattgccga tggcattgcc ggaagcgtcc ttgagggtct ccttgcggcg gatgtcgaga
     2941 cgcacgctcg cgtaaaattt caacgccttg ccgccgggcg ttgtttcggg attgccgaac
     3001 atcacgccga ctttttcgcg caactggttc gtgaaaatgc aggtggtctt ggacttgttc
     3061 aagatggccg tcaatttgcg caacgcctgg ctcatcaaac gcgcctgcat gcccatcgtc
     3121 gccatgccca tttcgccttc caattccgcc tttggcacca atgccgccac ggaatcgatc
     3181 acgatcacat ccagcgcgtt cgagcgcgcg agcgtttcgc agatgctcaa cgcctcttcg
     3241 ccgctgtccg gttgcgacac cagcaaatcg tccaggttca cgcccaattt ttttgcgtaa
     3301 gcggggtcca gcgcgtgctc ggcgtcaatg aacgcagcca aaccgccgcc cttttgggcg
     3361 ttggcgatga cgtgcaacat cagcgtggtt ttaccggaag attccggccc gaagatttca
     3421 acgacacgcc cgcgcggcaa tccgccgacg cccagcgcaa ggtccaatcc caacgcgccg
     3481 gtggggatga attggatgcc gaccttggcc cgcgcttcgc cgaggcgcat gatgctgccg
     3541 tcgccgtaag ccttgtggat gctggaaatg gcggcttcga ggtcgcggtc gcgcgtgggc
     3601 gaaacggcgc ggccattttc agagcctgcg gcgcgcggct cggcaacggc ggacttggcg
     3661 gacggttttt cagcggattt ttcaggtttc tcggcagttt ttggtggcat aataatttta
     3721 actttggaaa ttcgcttgcg actcaaagag tctggcaagc attagtgggt tggaaaaatt
     3781 agtcaatcgg tttcaacaat ttgcgtttcg ctattctcta tcctccatcc tcgtctatct
     3841 tcgccacccc ctcccccctc gaaaaaaagc gaaaaaaagc gagaaacccc gataatacgg
     3901 gccttcgcga cgagagcgaa tagaaaattt gccattgacc acgattggcg agtttttttg
     3961 ctcgaaagtt ggcgatgaat aatacgaccg cgcgttggcg tgatgggttg atgaccgaac
     4021 gcgcaatggc gaacagccgg tgcgactccg acaagccacg attttctgct caaacatgtt
     4081 ggatgaaaag tttgcatata acgaaagcat cgcatgggag gtcggacata aactgtccga
     4141 gtcaactttt taatttgtga cgttctgagc cggaatcctg ccgatgaaaa aggggagccc
     4201 acgcgtctcg cgtgtagtgt ttggcgtccc gccgaacact tcgtaacact gacatttctg
     4261 tccaccaaac cgtccaccag aagcaaagtc ctcgccggga cggctcggac ttcaacgaac
     4321 tcactttttc aacatcggtg atcggaagaa aatcagtatt acgatgtgtt cggcgagacg
     4381 ccgaacactg cacgccggag gcgtgcgctc ccctttcaac tgaatcgttc cgttgagcgg
     4441 ctcaaaggtt gaatgagcgc gattttagaa gtccggcatg tcggctgcgc gcgttccgct
     4501 gacccggttt cacgcgtagg ttcgaagcac ctcatgcacg cccgccacag ccgcaccgcc
     4561 ttcgccaacg gcggcggcgc agcgtttcac cgagccggag cgcacgtcgc ctgcggcaaa
     4621 aactcccggg cgacttgtct cgaatgggct gggcgcacgg ttcattccct tccatgccgg
     4681 acaatctgca acggcatggc cggttttgat gaagcctttt tcatcgcatt cgatctcagg
     4741 gggcaaccag ctcgtgcatg gatttgcgcc gatcattgaa aagatggcgg gtgtctggat
     4801 ggtgcgccgt tcatgggtca cagtgttttc cagctcggcg gcttcaagcg ccttgtcacc
     4861 gaacattctc acaatctgcg tgcgcgacaa tatctcgatg ttgtcctttg tctcgacccg
     4921 ccgcgaaaga tagacggaca tgctttttgc caacccgtcg ccgcggatga ccaacaagag
     4981 tttttcggca acttcagaga gaaacatcgc cgcttgtccc gccgagttgc cgccgccggt
     5041 gacgatcacc gttgagccgc gacaaagctg ggcttcgcgc cccgtggccg cgtaatagac
     5101 tccgcaacct tcaaaatctt cgcgtctgtc ggcctcaagc cggcgatatt cggcgcccgt
     5161 tgcgatgatg acgcacttgg cgcgcaaggt cgcgttgcaa ccctcggctt gcaacaaggc
     5221 ttgatgctcc ccatccatga acgccaaaga aagcgcctgg gcgggcgtgg cgaatttcgc
     5281 gccgaatcga taggcttgga gttgcgcgtt gtaagtcagg tctccgcccc gcacgccggt
     5341 ggggaagcca aagaagttct cgatcaacga agaagctccg gcttggcctc ccggcgcgta
     5401 gctttccaga acaaccgtgt gaagcccttc cgaagccgca taaaccgcgg ctgccaatcc
     5461 cgccggcccc gcgccaacaa tggccaggtc gcaggtgatt tcgccttcgt catcggtcgc
     5521 cgccgagcgc agcaatccgg caatccgcgc ggcttcgcgc aaggaaggat ggcgcagcgg
     5581 cattccattg gccgtaagca gcgcgggcag gtcgtggctt gccagatgca acttatcgca
     5641 gagcgcgcgc ccatattcac tttgaaaatc aatcaaccga tgcgggacat ggttcttgtc
     5701 gagaaaatcg tcgagccggc gtccttcgcg cgagtcatcc gacgccacga tgcgcaaccc
     5761 ggcaaattca cggtcgcgtt gcaggcgttt ccgccgcatg atgaatgcgc gcacgattgg
     5821 ctcgctcacg ttgggaattt ccgccaatgc ctttcgcaga cggtcggcgg gcacttcgag
     5881 gatttcgctt tcgtcggcgt tgccccgggc gctggccacc gcggccgtgc ccatgagcat
     5941 cgccacatcc ccgacaaaag tccggggacc ggcgttcgcg aggatttgct cctgaccgtc
     6001 aagcgagtcg aagacttcca ccgcgccacg caaaacaacc gttaggccaa ggtcgcgctg
     6061 gcccgctttg tacacgagtt cgccgcgctt caaaatacgc cgcgagccga gttgttcgag
     6121 catggccagt tggcggtcat tgagcttggg aaacgcgatg ctctccgtct cgcggtagaa
     6181 ttgctcgtct tcgtccggct cggttgcaat caattcgctt gccatgcgat ctccaattta
     6241 aacggggttt tctcctccat cgttcctgtc ggaactccaa aagcatcctc gacctgattc
     6301 atgcccttgt caattggact catcttggaa ctgttaccaa ccagggactg gaaagtcccc
     6361 tcaacccgca ggctggaaag cctgcgccac atcgcgcaga cattcttgtc tgcgggttca
     6421 ggcgactttc cagtgcctga attcctgcat ccaaaattct caatcatcaa cagttccagg
     6481 aagcgtccgc caattttcgg tgcatcttga cactgttctt gagaatgtac agttacggtt
     6541 ggttgacgct cgccctcacc caaaccctct cccccaggag agggctttca ccatttcccg
     6601 ctgttcggaa cattcccgcg atgggaatgg tgcaaaccat tgcgctaagg ataaagtggg
     6661 cggggccggt tctccttctc tcgtttttca caatgcattc aagaacagtg ttaggatgcg
     6721 cccggcgcat cggcgcactg ccccggagcg cgggtcgatg acccgcagca atgtgggaat
     6781 cccggctgtg tccgaattag tcgggcgtgt tctggttctg gtgcgttgct gcggctcatc
     6841 gagccgcgct ccgttttacc cggtgaacgc ccccaagctc aacccgtcta tcgggcctga
     6901 ccgacaaatc cggtacagtg ccaagatgcg ccgtggccat ttttctcact ggtgaaaaag
     6961 ctggaagttt gggccaaact ttgctaaagc aacggcatgt ttccatccgt tttttgcccg
     7021 gcgttgagtt tgcgcgcctt tttcagaaag ctggcccttt gcctctgcct gtttgcattg
     7081 gtcgtctgcg gattgacgtt ttcatcattc gcctcgacca acatgacgcc cattacagtg
     7141 actggctgga accgcgacgt gatgatcgag tcaaatgccg tcggcccgcc gttcacggct
     7201 tatgcgtccg aagtcgggcc gggggatgga aatgtttttt atcaaaccgg tttgccgggg
     7261 tatgtgtggg gtttgccgcc ttccggaaat ttcgccagca tgatcggcga cggcacgttg
     7321 ttctccttcc agccttacac ggcgaacaac gcgctggtgc tcagttctga aaccggcctg
     7381 acctccggca ctttgacgct ggtgacgccg gcgacttacg gcagcatcgc catcctttcc
     7441 gattccgtca acggcaccaa ccagaccggc tcgctgactt tgaatttcca ggatggcacc
     7501 agttttacaa cgacctattt cacgccggac tgggtgggcg ggacttatgg aactgcgtgg
     7561 ttcggcaacg gcaaaatcaa tctcaacaac aacggcgaca gcggcggaac gcaaagcccg
     7621 cgctatttcc aaacgactgt caatctgacc gcgttgctcg gcgcgacgaa caagccgctg
     7681 gcaagcatca ctttcgggca atcggtttcc aaggccacgg cgatttattg cgtcagcggc
     7741 gtgtttgcgg gcaacacttt gccggtcgcg ctgccggtga gcgtgaccgg tttcaatcgc
     7801 gacatcgtcg tcgaaaacac cgcgtcgggt ccgccttaca ccaattacgc ggtcgaactg
     7861 aatccggccg agggcaatgc attttatcaa agcggcctgc cgggaaaatc gtatggattc
     7921 ccgaccaacg gcatgttttc cagcgcggtg gatggaacga ttttccaact gcaatcatac
     7981 accgccaata acgcgctcgt cctgagcagc gacaccggca tcagccaggg cacgttgacg
     8041 ctgaccgcgc ctgcgactta cgacagcttg tcggtgatcg cgaattcggc cagtggcggc
     8101 ggcatcccgg ttgtcacaat caatttctcg gatggttcgt cgctggtgac gaatttcaac
     8161 gccccggact ggttcgccgg caatcaggac attgcgcttg cgggttttga acgcatcaat
     8221 cttaccaacg gtgcgacgca gggcgctccg gcgaacccgc gcctttacca gacgacttac
     8281 gatttgacgg cgctttacgg cgcgacaaac aagacggtca agaacctcac gttcaatcag
     8341 gccagcggcg caaaatccac ggcggtttat gccgtcagcg gcgttcgcgg caatcaacag
     8401 ccgagtgcga cttatcaatt tgcgggagtg acgaaccttc cggcctctgc aatccaggcc
     8461 cgttcggcga ccctcaacgg aaatatcatt tctttgggcg gttgcattcc agaggtgttc
     8521 gcgtattacg gcacgacgga tggacacaca aatccaacgg catggtcgca aaaaatgtat
     8581 cttggc
//
LOCUS       DASZMM010000367         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_962848, whole genome shotgun sequence.
ACCESSION   DASZMM010000367 DASZMM010000000
VERSION     DASZMM010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962848"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..578
                     /locus_tag="VG754_08915"
     CDS             138..578
                     /locus_tag="VG754_08915"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWF19377.1"
                     /translation="MKKKVLFVCIHNSARSQMAEAFLNQMCGGEFDAHSAGIEPGKLN
                     PLVIEAMREAGADISGNKTKSVSDMIKSDKQFEYIITVCDEASAERCPIFPGVAIRLH
                     WGFPDPSAFQGAHEEKLAKTRQVRDAIKQKIEQWCEQVCSVGVV"
     gene            593..1537
                     /locus_tag="VG754_08920"
     CDS             593..1537
                     /locus_tag="VG754_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWF19378.1"
                     /translation="MCDTRRAVIDVGTNSIKLLVADVLDGTVEPVWEQSEQTRLGTGF
                     FASNRLQPESIRQTARAVASFAAKARELNAASLRVIATSAARDAINPGDLTSAIERES
                     GLKVEIISGEQEAGWVYQGVTTNPELSRGPLLLLDVGGGSTEFILGEGGQKKFARSFQ
                     LGTVRLMEKLPHSDPPTMQELTATREFVKTFLEREVCSELKAALGGMANSGATMQLVG
                     TGGTAAILARMEGRLETYDRKLIEGMRISRARISAQVERLWSVPLASRRTIIGLPKSR
                     ADIILTGVVIYEAVMEIFGFSELRISSRGLRFAAVQSN"
     gene            complement(1564..2187)
                     /locus_tag="VG754_08925"
     CDS             complement(1564..2187)
                     /locus_tag="VG754_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HWF19379.1"
                     /translation="MLHQTQPAATAELLGDLRSLIEQIGSPEGLMTLAGEHHGRHITT
                     AAELRRFLQNYHSQVLHPCELPAIQRAFNHASRNELRELIAFDKELEKEMVLRNFVSA
                     SRRVGQNQLQRLRPLRDHRLVQRYLMAVEKGEATGWHTLVYGLTLSIYSVPLRQGLFG
                     YARQTTRGFIHSAARSLRLSEMESGNLLEEVSTGLPAAVEGLMTPLG"
     gene            2375..>4149
                     /locus_tag="VG754_08930"
     CDS             2375..>4149
                     /locus_tag="VG754_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HWF19380.1"
                     /translation="MAMVDFAIGIGGEAGQGIATPGDILARIFARRGLNLNAYNAYQS
                     IIRGGHTFLTIRVSDGPVRSMGDKLDVFIPLNQDTMDRHLRLLTAGACALFDVDKIKP
                     GVSATGVQLCPLPMKQLTAGNKLAGNTVAIAATLQMIGVEFKALEDALARIFKRKGDA
                     VIAENVGFARAGYDYAAANFRAFPFKAPNLGKGNAVLTGNMALAMGGIAAGVKFYAAY
                     PMSPSTGVLMYMASHARELGIMVRQVEDEIGVMNMIIGAAHTGCRSMCATSGGGFALM
                     TEAIGMAGMIETPVVCIDVQRAGPATGVPTKTEQGDLWQVLGAGQGDFPRIIVAPVDQ
                     LDGFKTIPELFNLCDKYQCPGLVLSDLLISEGTTSVNPKDLDFKPKIERGEVIFPNGN
                     GAANPFGGANDNAYLRYQNTESGISPRAVPGVPGHIHVAATDEHDEDGTLISDEYTNP
                     TKRRMMVEKRARKMETVLAQIAPPQLVGPANAAVTLVGWGSTAGVIREAVEKLAAEEG
                     IVANQLAIKWIVPFHADEIAHLLARSKRIIIVENNYSGQFARYLRSETGIAAHGHIRK
                     YDGEPFMPHHIVNAVKEQLGGQTELS"
BASE COUNT          908 a         1157 c         1251 g          833 t
ORIGIN      
        1 gcgcttgtct ccggtcattt cgaacatctt tggatttatc tggttgcgcc gacactcggc
       61 gcgtgcgccg gaatgctcgc ctgccgctgc gtgcgtgaga aggattgctg cgcgaaactt
      121 aagactgaaa ccgtgtcatg aaaaagaaag tcctctttgt ctgcatccat aacagcgccc
      181 gcagccagat ggccgaggcg ttcttgaacc agatgtgcgg cggcgaattt gatgcgcaca
      241 gcgccgggat tgagccggga aaactgaatc cgctcgtcat cgaggccatg cgggaagccg
      301 gcgcggacat ttccggcaac aaaaccaagt ccgtctccga catgatcaag tcagacaaac
      361 aatttgaata catcattacg gtttgcgacg aggcaagcgc cgaacgctgc ccgatttttc
      421 ccggcgtcgc gatccggttg cattggggtt ttcccgatcc atccgctttt cagggcgcac
      481 acgaggagaa gctggcaaaa acccgtcaag tgcgggatgc catcaagcag aagatcgaac
      541 aatggtgcga gcaggtgtgt tcagttggcg tagtttgata atgggattga ttatgtgcga
      601 cacaaggcga gccgtcattg atgtggggac gaactccatc aagctgctcg ttgccgacgt
      661 gctcgacgga acggtcgagc cggtgtggga gcagagcgaa cagacgcgct tgggcacggg
      721 atttttcgca agcaaccggc tgcaacccga aagcatcagg cagaccgcgc gggctgtcgc
      781 ttcgttcgcg gccaaggcgc gtgaactcaa tgcggcttcg ttgcgcgtca tcgcgaccag
      841 cgcggcgcgt gatgccatca atcccggcga cctgacttcg gcgattgagc gtgaatcggg
      901 gttgaaggtt gagatcatct ccggcgagca ggaggcgggc tgggtgtatc aaggcgtcac
      961 gacgaacccg gaactttcgc gcgggccgtt gctgttgctg gatgtcggcg gcggcagcac
     1021 ggaatttatt ttgggcgaag gcgggcaaaa aaaattcgcg cggagtttcc agttgggcac
     1081 ggtgcgattg atggagaaat taccgcacag cgatccgcca accatgcagg aattgactgc
     1141 gactcgcgag tttgtgaaaa catttctcga acgcgaagtg tgttcggaat tgaaagcggc
     1201 gcttggcggc atggcaaatt ccggcgcgac gatgcaactg gttgggacgg gcggcacggc
     1261 cgcaattctg gcgcgcatgg aaggacggct cgagacgtat gatcgcaaat tgatcgaggg
     1321 gatgcgcatc agccgcgcgc gaatcagcgc gcaagtggaa cggctctgga gtgtgccatt
     1381 ggccagccgc agaaccatta tcggcctgcc gaaaagccgc gccgacatca tcctgaccgg
     1441 cgtggtgatt tatgaagccg taatggaaat tttcggcttc tccgaattgc gcatcagcag
     1501 ccgcggcctc cgtttcgccg ctgtgcaaag caactgagac gtcatttttt cactgggtgt
     1561 tattcatccg agcggcgtca tcaaaccctc caccgccgca ggcagaccgg tgctgacttc
     1621 ttccagcagg ttgcctgatt ccatttcgga aagccgaagc gaacgcgcgg cggaatgaat
     1681 gaacccgcgg gtggtctggc gcgcataacc gaaaagtccc tgccgcaacg gcaccgaata
     1741 aatggagagc gtcaatccgt aaaccaacgt gtgccagccg gtggcttcgc ctttctcgac
     1801 tgccatgaga taacgctgca cgagccggtg gtcgcgcagg gggcgaagcc gttgcaactg
     1861 gttctggccg acgcggcggc tggccgagac gaaatttcgc agcaccattt ctttttccaa
     1921 ttccttgtcg aaagcgatga gttcgcgaag ttcattgcgg ctggcgtggt tgaacgcgcg
     1981 ctggatggct ggcaattcgc aagggtgaag gacctgtgaa tggtaatttt gcaagaaccg
     2041 gcgcaactcg gcggctgtgg tgatgtgccg gccatggtgc tcgccggcga gcgtcatcaa
     2101 gccttcaggc gagccgatct gctcgatcag cgaacgtaag tcgccgagta attccgccgt
     2161 tgccgctggt tgtgtctgat gcaacatgtt ctgttaaata tctgtttata gtcattaacg
     2221 ctgccccgct tcccggttga atgccagcag taaaaatgcc atcgcgaaaa tttatcgctt
     2281 gcattcgcct aatgttattg attaaagtcg cagtcattcc attgctgggc ctaactgacc
     2341 cttaaccaaa aacgacctaa ccgggagaaa tgtcatggcc atggtagatt ttgccatcgg
     2401 tatcgggggc gaagcaggac agggaatagc aacaccggga gacatcctgg cgcgcatctt
     2461 cgcgcgtcgc ggcctcaacc tgaacgccta caacgcctat caatccatca tccgcggcgg
     2521 acacaccttc ctgaccatcc gcgtcagcga cggccccgtc cgcagcatgg gcgacaagct
     2581 cgatgtgttc attccgctca accaggacac gatggaccgc catctgcgcc tgttgacggc
     2641 cggggcgtgc gcattgttcg acgttgataa aatcaagccc ggtgtttccg ccacaggcgt
     2701 ccagctttgt ccgctgccga tgaaacaatt gaccgccggg aacaaactcg ccggcaacac
     2761 cgttgccatc gccgccacgc tccagatgat cggcgttgaa ttcaaagcct tggaagacgc
     2821 gctggcccgc attttcaaac gcaaaggcga cgcggtgatc gccgagaacg tcggtttcgc
     2881 gcgcgccgga tacgattatg cggcggcgaa tttcagggcg tttccattca aggcgcccaa
     2941 tctcggcaag ggcaatgccg tgctcaccgg caacatggcg ttggccatgg gcggcattgc
     3001 ggcaggcgtg aaattttacg cggcctatcc gatgagtcct tccacgggag tgttgatgta
     3061 catggccagt cacgcccggg aattgggcat catggtgcgg caggtcgagg atgaaatcgg
     3121 ggtgatgaac atgatcatcg gcgcggcgca taccggttgc cgttcgatgt gcgcgacttc
     3181 cggcggcggt tttgcgttga tgaccgaggc gatcggcatg gcgggaatga ttgagacgcc
     3241 ggtggtttgc attgatgttc aacgcgccgg tccggcgaca ggtgtgccga ccaagaccga
     3301 acaaggcgac ctctggcagg tgcttggcgc aggccaagga gattttccgc gcatcatcgt
     3361 cgcgcccgtg gaccagcttg acgggttcaa gacgattcct gaattgttca acctgtgcga
     3421 caaatatcaa tgccccggct tggtgttgtc ggacttgctc atttcggaag gcacgacgag
     3481 cgtcaatccc aaggaccttg atttcaaacc gaagatcgag cgcggcgaag tcatttttcc
     3541 gaacggcaat ggcgctgcca atccattcgg cggcgcgaat gacaatgcat atctgcgcta
     3601 tcaaaacacc gaaagcggca tttcgccgcg cgcggtgccg ggcgttccgg gacacatcca
     3661 cgtggctgcg acagatgaac acgacgagga cggcacgttg atcagcgacg aatacaccaa
     3721 ccccaccaaa cgccgcatga tggtggaaaa gcgcgcgcgc aaaatggaaa ccgtccttgc
     3781 gcaaatcgcc ccgccgcaac tcgtcgggcc ggcaaatgcc gccgtcacac ttgtcggatg
     3841 gggttcgacc gccggtgtca tccgtgaagc ggtggaaaaa cttgccgctg aggaaggcat
     3901 cgtggcgaac caactcgcca taaaatggat cgtgccgttt cacgcggacg aaatcgcgca
     3961 tctgctcgcg cgcagcaagc gcatcatcat tgttgaaaat aattacagcg gccagttcgc
     4021 gcgttacttg cgttccgaga ccggcatcgc cgcgcacggc catatccgca aatacgatgg
     4081 cgagccgttc atgccgcatc acatcgtcaa cgccgtgaag gaacaactcg ggggccaaac
     4141 cgaactttc
//
LOCUS       DASZMM010000368         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_141887, whole genome shotgun sequence.
ACCESSION   DASZMM010000368 DASZMM010000000
VERSION     DASZMM010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141887"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1868
                     /locus_tag="VG754_08935"
     CDS             15..1868
                     /locus_tag="VG754_08935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWF19381.1"
                     /translation="MILENAKITTLDRAKPEATSLVVKNGRIVGVDNAREYQRGPDTK
                     VIDLQGRRVIPGLNDSHMHIIRGGLTYNLELRWDGVPSLADGLRMLKEQAQRTPPGQW
                     VRVIGGWSEFQFAERRMPTLDEINAVSPETPVFVLHLYCRGMLNKAALRACGYTKDTP
                     NPPAGEIQRDGNGNPTGLLIARPNAGILYATVGKQPKLPPEQQMNSSRHFMRELNRLG
                     LTSLVDAGGGSQIYPDDYAIIEELHKRGEMTVRMAYNIFTQKPKQEKEDFLRCMKLTG
                     PGKGDDFYRCNGAGEMLVYSAADFEDFLEPRPDLPSNLEKDLKEVVSLLAANKWPFRI
                     HATYDESISRMLNVFEEVNREIPFKGTNWFFDHCETISDRSLERVKALGGGIAIQDRM
                     AFQGEYFLDRYGSKAAERTPPVRRMLDLGIPVGAGTDATRVASYNPWLSLYWLVTGKT
                     VGGTALYSEANRLSREEALMLYTKGSSWFSGESGKKGAIVIGQLADVTALTEDYLSVA
                     EEKIKGIESVLTIVGGKIVYAVQEFQNLDPAPLPVLPDWSPIKVYGGYGAPLDIRKAA
                     RAGVPMPQHQHNAECHHHGCAHAAHQLLAGIEAASIRYAEFWGLGCDCFTF"
     gene            complement(1940..2083)
                     /locus_tag="VG754_08940"
     CDS             complement(1940..2083)
                     /locus_tag="VG754_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19382.1"
                     /translation="MPFIDLAFCLKPVGQVVSVFATALMPKFMRSPGNLLFQACFLVN
                     MEN"
     gene            2111..2608
                     /locus_tag="VG754_08945"
     CDS             2111..2608
                     /locus_tag="VG754_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWF19383.1"
                     /translation="MNLLKKSLTMEPNGVAATFGHLFLRVSAGTMIFYIHGLHKLEGW
                     IAYLQHGTPWTLAEEVAGMHFPAPLASAVAATLVRFICSLFIIVGAFTRINALLLFGA
                     LSGAILQNLLAGRDPQLAILYSLVVLTLVFMGGGRISLDARLFAKAEQSTSRNAGMGR
                     ELNSI"
BASE COUNT          731 a          823 c          803 g          600 t
ORIGIN      
        1 catccgcgcc ggacttgatt cttgagaatg ccaaaataac caccctcgac cgggccaagc
       61 cggaggcgac aagtctggtt gtgaaaaacg ggcgaattgt cggagtggac aacgcgaggg
      121 aataccaacg gggaccggac acgaaggtga ttgatttgca gggtcgccgg gtgattcccg
      181 gattgaacga ttcccacatg catatcatcc gcggtggcct tacctacaac ctcgagcttc
      241 gctgggacgg tgttccgtcc ctcgcggacg gattgcgcat gctcaaagag caggctcagc
      301 ggacaccgcc cggccaatgg gtccgcgtca tcggcggttg gagcgagttc cagttcgccg
      361 aacggcgcat gcccacgctc gatgaaatca acgccgtctc ccccgaaacg cctgtcttcg
      421 tcctgcactt gtattgccgc ggcatgttga acaaagccgc gctgcgggcc tgcggctaca
      481 ccaaagacac tcccaatcca ccggcgggtg aaatccagcg cgacggaaac ggcaacccca
      541 ccggactcct catcgcccgc cccaatgccg gaattcttta cgcgaccgtc ggcaagcaac
      601 ccaagctgcc gccggaacaa cagatgaact cttcccgcca tttcatgcgc gagctcaacc
      661 gccttggcct caccagcttg gtggacgcgg gcggcggctc tcaaatctac ccggacgact
      721 atgcgatcat cgaagaactg cacaagcgag gagagatgac cgttcgcatg gcctataata
      781 ttttcacgca aaaacccaaa caggagaagg aggatttcct gcgctgcatg aaactgacgg
      841 ggccgggcaa aggcgacgat ttctatcgct gcaacggggc tggagaaatg ctggtttatt
      901 cagcggcgga tttcgaggat ttcctggaac cccggcccga ccttccgtcc aacctggaaa
      961 aggacttgaa ggaggtggtt tcgctcttgg ctgcaaataa atggccgttc cgaattcacg
     1021 ccacctatga cgaaagcatc tcccggatgt tgaacgtgtt cgaggaggtc aaccgtgaaa
     1081 tccctttcaa aggaaccaat tggttcttcg atcattgcga aaccatctcg gaccgcagcc
     1141 tcgaacgtgt caaagcgttg ggaggaggca ttgcgatcca agaccgcatg gccttccaag
     1201 gagagtattt cctggaccgc tatggcagta aggcggcgga acggactcca cctgttcggc
     1261 ggatgctcga tttgggcatt cccgtggggg cgggcacaga tgcaacacga gtggccagct
     1321 acaacccgtg gctttccctt tactggctcg tgaccggaaa aaccgtcggc ggcactgcgc
     1381 tctattccga agcaaatcgt ctgagccggg aagaagcgtt gatgctttac accaaaggca
     1441 gcagttggtt ttcgggcgaa agcggcaaaa agggagccat cgtcatcggc caactggcgg
     1501 acgtcaccgc cctcaccgaa gattacctgt cagtggccga agagaaaatc aaggggattg
     1561 aatccgtcct cacgattgtt ggtgggaaga ttgtttacgc ggttcaggaa tttcagaacc
     1621 ttgacccggc accactgcca gtgctgccgg actggtcacc cataaaggtc tatggtggat
     1681 atggcgcgcc gttagacatt cgcaaagctg cgcgagcggg ggtgccgatg ccgcagcatc
     1741 agcacaatgc cgaatgccat catcacggct gcgcccatgc ggcccatcaa cttctcgccg
     1801 gtatcgaggc cgccagcatt cgttacgccg aattctgggg attgggttgc gattgtttta
     1861 cgttttgagg atcccgacga caacgtcaac ctcaaccacc acccaaatga ataaatcaaa
     1921 aacttcaaaa acgactgcgt caattctcca tgttgacgag aaagcaagct tggaagagca
     1981 aattgcccgg cgagcgcatg aactttggca tcaacgcggt cgcgaacacg gacacgacct
     2041 gaccgactgg cttcaggcag aacgcgagat caatgaatgg catcaaaggc ggctcaaaag
     2101 ggattgaaaa atgaacttgt tgaaaaaatc cctaacgatg gagccgaacg gcgtggctgc
     2161 aacttttgga catctcttcc tccgcgtttc agcgggaacc atgattttct acattcatgg
     2221 cctgcacaaa ctggaaggct ggattgccta tctccaacac ggcacgccgt ggacattggc
     2281 cgaggaagtt gcggggatgc atttccctgc gccattagcg tcagcagttg cggcgacact
     2341 ggtgcggttc atctgttcat tgttcatcat cgttggagcg ttcacgcgca tcaatgccct
     2401 tttgctattt ggcgcattga gcggcgcaat ccttcaaaac cttctggcag gccgcgatcc
     2461 gcaactggcg attctatatt cactcgtggt cttgaccctc gtgtttatgg gtggaggcag
     2521 aatatcgctt gatgccaggt tgtttgcaaa agcggaacaa tcaacctcgc gaaatgccgg
     2581 gatgggaagg gagttgaatt caatatgaaa gcgaatccaa ccgaacaaga gcccgaaatc
     2641 ttgaaattaa gaccggacat aacgggctgt gtcattccgt gcctgcccaa catcatccgt
     2701 gcaagggcat accagctctg atttgttcac ggaagtctcg cgcggcattg ataaatggct
     2761 gtggtttgtt gaagcgcact cccaagcggc caaatgacga tgaaaatttg aaaccgtgaa
     2821 cgtcgataac gaaaccacgg attcacatga acaacaaaat aaaattgaac agccgcattc
     2881 gctaagcaaa aaatcaacca cattgcctgc tggtgccagc gctcccgact tcacgctcaa
     2941 aagcacgccg gaccagt
//
LOCUS       DASZMM010000369        13412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1532520, whole genome shotgun sequence.
ACCESSION   DASZMM010000369 DASZMM010000000
VERSION     DASZMM010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13412
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532520"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VG754_08950"
     CDS             <1..531
                     /locus_tag="VG754_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HWF19384.1"
                     /translation="SRNNIRTSLADEGVECILNGLYLTRDEQLADHHMVVEHAKPHCN
                     SHEYYNGILDGHSKGVFHGRILVRPDAQKTDAKQTNKNLLLSENATADTKPQLEIYAD
                     DVKCTHGATVGQLNDESIFYLRARGIGLETARRMLIHAFAGEIIDRIRYAPVREEMDK
                     LVWERLEQSPLIGEKK"
     gene            650..1096
                     /locus_tag="VG754_08955"
     CDS             650..1096
                     /locus_tag="VG754_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014148483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWF19385.1"
                     /translation="MTDELDDLYQQVILDHCKSPRNFHALEGANRKGEGFNPLCGDNY
                     TVFLKMNGDIVEDISFTGSGCCISKASASLLTTAVKGKTKNEVKALFEKIHDLITTGR
                     GDEAGVGKLAVFAGVHKFPARVKCAILSWHAVLAAVEGKNESVSTE"
     gene            1114..1722
                     /locus_tag="VG754_08960"
     CDS             1114..1722
                     /locus_tag="VG754_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19386.1"
                     /translation="MKKLIVLATLSLIMLGASCRRSEQSSASVEEGVLTFDAEQTASN
                     QTDTNTMATWLASYDSGGKKAQFRIEITIPKAKREGFAFGTGAFYHDTNSSSSVLLAD
                     LAKMLQAKKPVPCPTTTNKLSFATAILGENASKGNGKDSLAGSFTSDPKGDWTVLKVF
                     LPDGDNEGEVFLNLNSKLRKGEFTIKDPDYGDTVVLELARVL"
     gene            1797..2363
                     /gene="sufT"
                     /locus_tag="VG754_08965"
     CDS             1797..2363
                     /gene="sufT"
                     /locus_tag="VG754_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HWF19387.1"
                     /translation="MQTNEPVTLKRDVEASVVPVGTKVTLQAGEQAYITQSLGGTYTV
                     IVNGNMFRIEGKDADALGMEVTAKPETPNPLANGPVNREQLEKEIWNQMRSCYDPEIP
                     VNIVDLGLIYDCNIEPIAGAQNSYRVDVKMTLTAPGCGMGPTLQQDVQNKLLSIEAVD
                     DVNVELVWEPQWNQGMLTEAAKLQLGLL"
     gene            2363..3625
                     /locus_tag="VG754_08970"
     CDS             2363..3625
                     /locus_tag="VG754_08970"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWF19388.1"
                     /translation="MITETGILETPKKMIDWAKLRADFPILDQKVHGHPLIYFDNAAS
                     SQKPRVVIETVSRYYERDNANVHRGIHELSNRATNAFEASRARVAKFINAKSDNEIIF
                     TRGTTEGINLVAQSWGGKNIKAGDKILLTEMEHHSNIVPWQMLAERTGAKLVYLPVTG
                     DEGLLDLSKLDALLTKEVKLFAFTHISNTLGTVNPVAELCERARKLGVTTLVDAAQSA
                     GHRPIDVQELGCDFLVFSGHKICGPTGIGVLWGREEILNSMPPYQGGGEMILNVDFFK
                     TTYKQAPHRFEAGTPDISGVIGLHAALDYLDAIGRENIFKHDQELAGSAYEQLARLNN
                     IRLFGPKTGRAGLVSFLLNDVHAHDVVTVADQRGVALRGGHHCNQPLMKRLGVPSTAR
                     ASFYFYNTKAEVDRFIEVVKEIQKFFGS"
     gene            3677..3946
                     /locus_tag="VG754_08975"
     CDS             3677..3946
                     /locus_tag="VG754_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19389.1"
                     /translation="MKEVTIQEAQGKLAQLVAEAQRGELIVLTDGENKVTLHPGKPLD
                     LEQDSPELEAELLKAANGPFTPYQPGELRELANKVLEKHRAKAQR"
     gene            4281..4817
                     /locus_tag="VG754_08980"
     CDS             4281..4817
                     /locus_tag="VG754_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWF19390.1"
                     /translation="MTITPQSPMREVLEIYPGAQRALFRKYHIGGCSSCGFQPTETLA
                     QVCARNNNLNVDEVLQHIQTSHAEDEKILILPKQLAELRAKNPSLKLLDVRSREEFEA
                     THIEGSTLMSQPTVQEIMAHWPRTEEFVIVDHTGKMGLDAAAYFAGHGFERIRCLRGG
                     IDAWSREVDSNVRRYQLA"
     gene            4901..5389
                     /locus_tag="VG754_08985"
     CDS             4901..5389
                     /locus_tag="VG754_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWF19391.1"
                     /translation="MTEDLQKRVQQALQNPQNMGEMAGADSVGTVGNSECGEMLRVWV
                     KFKEENGKKVIDRATFQSFGCETAIAVASLATELIRGKTADEALAMKTEELAGELGPL
                     PPMKIHCAQLVEGALRSALEPESGAPKPAVAAHAQPAANSNLLDNFTKPKEGGVRVKF
                     LD"
     gene            complement(5422..5919)
                     /locus_tag="VG754_08990"
     CDS             complement(5422..5919)
                     /locus_tag="VG754_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19392.1"
                     /translation="MRLTIKIGAAILAFIPMVLMFAAVGLSSKGLISQALGIKIFAGC
                     GIFYFPVLSIMSAVNTFHVGKIVQLSVLLIGLFSWSLLIAWMLWKIAGTLQGEEEPRK
                     KFDWIGFQVRFVCGFIVGCLSGWRFVRYTTSTKTLVVATLLTGFTFGVAFGLMRTREL
                     WSGNF"
     gene            complement(5933..7774)
                     /locus_tag="VG754_08995"
     CDS             complement(5933..7774)
                     /locus_tag="VG754_08995"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWF19393.1"
                     /translation="MKLCTIFNSLALGSVLFFATHGTLTAAENPPPADVIICNAKVLT
                     VNSNFTTAQAIAVRGNQIVAIGKDKQILRFKGPETRVIDGHEHTVMPGLYDNLVDSYQ
                     ASVSTLNGPLPMFDSIARAKDFIHDQAAQKPAGAWIVVERAFPTRLKECRLPTKAELD
                     AVAPKNPVYWNCGDLAVVNTKALEVLHLTTTTTNPSGGEIVLDPVTRKPTGLLRHASS
                     VVKLPPAAVTATAQQHRDALKHLYGLYNEQGITSIGESDAEPEVINLFRDMAASNELT
                     VRINCTRYVAPGATIEETTARLDALTNTPADKPQYGPTGAGDDWVRIGPLLAQVDGDL
                     LDSTAYLRTPWGIGPAFQITEPAYRGKLVTDPDVLTSFFTAAAQRGWQITADCTGDAG
                     LDQLLNCYQKVQFKTDISQRRFLLRHANFQAEQDWNRCVKLGVGAEMQPICLYHDGSG
                     MLKLLGEKRLRNFEAFKGWFDHGLLIGAGSGHQSGLNSLDSANFWNPWYGLWVTLARQ
                     TAQNDGIYVKEEQLSREQAIQFYTMNNAKLHFEEEKKGSLEIGKLADLIVLDRDIMKC
                     AVDDVRDTQVQLTLLDGKIVWEAKQPPAPVSQAPEKKLASLPPKRSE"
     gene            7905..8441
                     /gene="mscL"
                     /locus_tag="VG754_09000"
     CDS             7905..8441
                     /gene="mscL"
                     /locus_tag="VG754_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWF19394.1"
                     /translation="MSLLKSDNHEPLKQKREPMKDFREEFSLANAKKKLGILWEFKQF
                     AMRGNVVDLAVGVVIGAAFGKIVTAMVSDFITPIIGKLTSGGADLKNKFISLEPSKTE
                     GIKSLADAQKAGAVIAWGDFVTCIVDFLIVAFCIFLIVKLFNRLNMASAAVAPPAEPT
                     PTEKLLGEIRDELKKKPV"
     gene            8528..8683
                     /locus_tag="VG754_09005"
     CDS             8528..8683
                     /locus_tag="VG754_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19395.1"
                     /translation="MVIAAIIVGAGVFVELLAILKAPLGYQDDTGFHVGASGAENEEG
                     QPWLNPS"
     gene            complement(8935..10725)
                     /gene="carB"
                     /locus_tag="VG754_09010"
     CDS             complement(8935..10725)
                     /gene="carB"
                     /locus_tag="VG754_09010"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWF19396.1"
                     /translation="MNRELLTKAKSLGFSDCQIAHLTGTSEDAVRAERKKLGLVPSYR
                     LVDTCAAEFEAYTPYYYSTYDCGDDEIKPTTTRKVMILGGGPNRIGQGIEFDYCCVHA
                     AFALKEDGFETLMVNSNPETVSTDYDTSDKLFFEPLTLEDVLHIYEREKCWGAIAQFG
                     GQTPLNLALGLQKNGVNIIGTSPQSIEIAEDRKLFAAMLRKLDIPQPPNGLATNEAEA
                     LEAAHKLGYPILVRPSFVLGGRAMQIVYSDSELTHYMRFAVEASPERPVLVDKFLEDA
                     TEVDVDCIADVGHFANPNEGTIVIGGMLEHIEYAGVHSGDAAMVLPPHTLSQKVIEII
                     RQYTHAMARELKVIGLMNVQYAVKGDVVYVLEVNPRASRTVPFVSKAIGVPLAKLAAK
                     VMAGKKLTELNFTKEIWTEYWAVKESVFPFNRFHGQDILLSPEMRSTGEVMGLDTDLG
                     IAYAKSQMAANAPLPLSGRVFISVSDAHKSEVADVAQEFAKLGFELISTSGTAAVLEK
                     AGLKVQRIYKLAEGRPNALDLLKNREIQLVINTPAGQTPRADEIKIRTTAVYTGTPIM
                     TTLSGAKAAALGIAALKKSGYGVRTVQEYH"
     gene            11150..11710
                     /locus_tag="VG754_09015"
     CDS             11150..11710
                     /locus_tag="VG754_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HWF19397.1"
                     /translation="MKTKPTSLTPYVALLRGINVGGKNMLPMADLAKMFVTARCTDVR
                     TFIQSGNVIFNATPDLAGRIAEVISAQIARKFGCKIPVVLRSARQIEETAVNNPFIKA
                     GAPEEMLHVMFLADLPKSDKVAQLDAKRSPPDEFIVRGQEIFLKLPNGAARTKLTNAY
                     FDSKLATISTGRNWRTVLKLLELVKA"
     gene            complement(11758..13029)
                     /locus_tag="VG754_09020"
     CDS             complement(11758..13029)
                     /locus_tag="VG754_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWF19398.1"
                     /translation="MAELVGNLLVAQSGGPTAVINASVAGVIQEAGKHECIEEIYGGL
                     NGILGLLNEDLIDINEEKARTIEGLKYTPAAALGTCRYKLDFKKKAEQAAKDMDRLFE
                     VFQAHNIRYFFYAGGNDSQDTANKIHEQAVARGYEMRIIGVPKTIDNDLPHTDHCPGY
                     GSVIKYNATTVMEVGEDVRSMATDGGFCCLIEVMGRSAGWIAAGTVLAKRSPADAPHI
                     ILLPEIKLDEAAFLARVKETIEQHKYCIVVVGEGVKNAAGEEIAADKSRLDAFGHPVL
                     SGAADRLAELVQGKLGTKTRTVKLGYAQRAAAHYASATDATEAAACGEAAVRAAIAGQ
                     SGFMVKIVRTQSEPYKWTTGLQPLGDIANVEHLLPRDWISEDGFMPTEKFVEYARPLV
                     EGEMKVPTEGGLPKFVSLEKVRVEKKLPPRG"
     gene            complement(13017..>13412)
                     /locus_tag="VG754_09025"
     CDS             complement(13017..>13412)
                     /locus_tag="VG754_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19399.1"
                     /translation="VSSTNFPPSSRRNFRLIPDKKINFFEGKLIVLTLLRHVFAIRYP
                     RLGVSLQGKAAEDCRLLRVVSRRSACFRNFFVARKTKWWKAFMAGRVCELPFTEMLPK
                     GKVRIRFLRFKPTDGGNRVVLIGSNIWLN"
BASE COUNT         3240 a         3500 c         3683 g         2989 t
ORIGIN      
        1 tcgcgcaaca acatccgcac cagcctcgcg gatgaaggcg tggaatgcat cttgaacggc
       61 ctgtatctca cgcgcgacga acaactcgcc gatcatcaca tggttgtcga gcacgccaag
      121 ccgcattgca acagccacga atattacaac ggcattctcg acggccattc caagggcgtt
      181 tttcacgggc ggattttggt gcgtcccgac gcgcaaaaaa ccgacgccaa acagacgaac
      241 aaaaatctgt tgctctcgga aaacgccact gcggacacca agccgcaatt ggaaatttat
      301 gccgacgatg tgaaatgcac gcacggcgcg accgtcggcc agttgaacga cgagtccatt
      361 ttttacctgc gcgcccgcgg catcggattg gaaaccgcgc gccgcatgtt gatccacgcg
      421 ttcgccgggg aaatcattga ccgcatccgc tatgcgcccg tgcgcgagga aatggacaag
      481 ctggtctggg aacggctgga gcagagtccg ctgataggcg agaagaaatg aaaggcggaa
      541 cgcacatgtt tggtgtccac gctttagcgt gtctgacgcc atttggagac atgctaaagc
      601 atggacaccg aacgatcgcg ggcattcctc ccaaaaataa ttttacgtaa tgaccgacga
      661 actggacgat ctttatcagc aggtaatctt ggaccattgc aagagtccgc gcaattttca
      721 tgcgctcgaa ggcgcgaacc gcaagggcga aggctttaat ccgctctgcg gcgataatta
      781 cacggttttc ctgaaaatga acggcgacat cgtggaagac atcagcttca ccggttcggg
      841 ctgctgcatt tccaaagcgt cggcctcgct tttgaccaca gccgtgaagg gcaagacgaa
      901 gaatgaagtg aaggcgttgt tcgaaaaaat ccacgacctc atcaccaccg ggcgcggaga
      961 tgaggcgggt gtcggcaagc tggcggtgtt cgccggcgtg cataaattcc cggcgcgggt
     1021 gaaatgcgca attctttcct ggcacgctgt gttggccgcc gtcgaaggaa agaatgaatc
     1081 cgtgagcacg gagtaactca gttgattgca tccatgaaaa aactaatcgt tctcgcgacg
     1141 ttgagtttga taatgctagg cgcaagctgt cggcggagtg aacaatcctc tgcttccgtt
     1201 gaagaaggtg ttttgacatt tgacgctgaa caaaccgcct caaatcaaac ggacacaaac
     1261 accatggcaa cctggctggc ctcgtacgat tctggcggaa aaaaagcgca gttccgcatc
     1321 gagatcacca tccccaaggc aaaacgcgaa ggatttgctt ttgggacagg cgctttttat
     1381 catgacacca attcctcatc ctcagtcctt ctcgcggacc tggcaaaaat gttgcaggca
     1441 aaaaaaccgg taccctgccc gaccacgacg aataagttgt cctttgccac cgccatcctc
     1501 ggcgaaaatg caagcaaagg taacggaaag gattccctgg ctggctcgtt cacttccgat
     1561 cccaaaggcg actggacggt tttgaaagtt tttctgccgg atggagacaa tgaaggcgaa
     1621 gtttttctca atttgaactc caaattgcgc aagggcgaat ttaccatcaa ggaccctgat
     1681 tatggcgaca cggttgtgct tgaattggcc cgtgttttgt gagtcgaaat caataactct
     1741 gacgactttc atgcggtgaa gacaagcgcc cgttattctg gtatagtctg attggtatgc
     1801 aaaccaacga accagtcact ttgaagcgcg acgtcgaggc gtcggtcgtt ccagtgggga
     1861 cgaaggtcac attgcaggca ggcgagcagg cttatatcac gcaatcactc ggcgggactt
     1921 acaccgtgat tgtgaacggc aacatgttcc gcatcgaagg caaggacgcc gatgccctgg
     1981 gcatggaggt gacggcgaag cccgagacgc caaatccatt ggcaaacggc ccggtgaatc
     2041 gcgagcagtt ggaaaaggaa atttggaacc agatgcgcag ttgttatgat ccggaaatcc
     2101 cggtgaacat cgtggacctg ggtttgattt acgattgcaa catcgagccg attgccggcg
     2161 ctcaaaacag ttaccgcgtc gatgtgaaga tgactttgac cgcgccgggt tgcggcatgg
     2221 gaccgacctt gcaacaggac gtgcagaaca aactgctttc aatcgaagcg gtcgatgacg
     2281 tcaatgtgga actggtttgg gaaccgcaat ggaaccaggg aatgctgacc gaagcggcga
     2341 aattgcagtt gggattgttg tgatgatcac tgaaaccggc atacttgaaa cgccaaaaaa
     2401 gatgattgat tgggcgaaat tgcgcgcgga ttttccaatc ctcgaccaga aagtccatgg
     2461 tcatccgctg atttatttcg ataatgccgc cagcagccag aagccgcgcg tggtgatcga
     2521 gactgtgagc cgttattacg agcgcgacaa cgcgaacgtc caccgcggaa tccatgaatt
     2581 gagcaatcgc gcgacaaatg cattcgaagc ttcccgcgcg cgggtcgcaa aattcatcaa
     2641 cgcaaaaagt gacaacgaaa tcattttcac gcgcggcacg acggaaggca tcaatcttgt
     2701 ggcgcaatcc tggggcggca aaaacatcaa ggccggtgac aaaattttgc tcacggagat
     2761 ggagcaccac agcaacatcg ttccgtggca aatgctcgcc gagcgcaccg gggcaaagct
     2821 ggtttacctg ccggtgaccg gtgacgaagg attgctggat ttaagcaaat tggatgcgct
     2881 gctcaccaaa gaagtgaagc tgttcgcatt cacgcacatt tccaacacgc tgggaaccgt
     2941 caatccggtg gccgaacttt gcgaacgcgc gcgcaagctt ggtgtcacca cgcttgtcga
     3001 tgcggcgcaa agcgcggggc atcgtccaat tgacgtgcag gaactcgggt gcgatttcct
     3061 ggtgttctcc ggccataaga tttgcgggcc gactggcatt ggggttcttt ggggacgcga
     3121 ggaaatctta aattcgatgc cgccgtatca aggtggcggc gagatgatct tgaatgtgga
     3181 ttttttcaag accacctata aacaagcgcc gcaccggttt gaagcgggca cgccggacat
     3241 ttcgggcgtc atcggattgc acgccgcact ggattatctc gatgccattg gccgcgaaaa
     3301 cattttcaaa cacgatcagg aactggctgg ttccgcgtac gagcaattgg cccggttaaa
     3361 caacatccgc ctgttcggcc ccaagacagg tcgcgcgggt ctggtaagtt ttctgttgaa
     3421 tgacgtccat gcacacgatg ttgtgacagt ggctgaccaa cgcggtgtgg cgttgcgcgg
     3481 cggtcatcat tgcaaccagc cgctcatgaa acgcctcggc gttccttcga ccgcgcgggc
     3541 aagtttttat ttttacaaca ccaaggccga agtggaccgg ttcatcgaag tggtgaagga
     3601 gatacaaaag ttttttggca gttaagtttc ccaaaagctg cttgctggcc ttgggccaaa
     3661 gcattaaagt gtcagcatga aagaagtcac catccaggag gcgcagggta aattggcgca
     3721 actcgttgcc gaagcccaac gcggcgaatt gattgtgctc acggatgggg aaaacaaggt
     3781 gactttgcat ccaggaaagc ctctcgattt ggagcaagac agtcccgaat tggaagcaga
     3841 actgctcaaa gcggcgaacg ggccgtttac accataccaa ccaggagagc ttcgcgaatt
     3901 ggccaataaa gttttggaga aacaccgggc aaaggcgcag agatgagttt cgatttgcgc
     3961 cgggaagcct ggtcagacga agatttccaa gctcaatacg aatggtactt aatacaagca
     4021 ggtgaggaga tagcggaacg atttctaaaa gcgttggagg acacattgaa gcttttgcag
     4081 gaacgacctg atttaggccg tcgcagaaga tttcgacatc cggctcttcg caatcttcga
     4141 tccttccaag ttgcgcggcc gtttgagcga atcctaattt tttatcgcct cacagaaggc
     4201 actgtggaaa ttttccggat aatggatggt atgcgcgatt tgccgcacag actggtggaa
     4261 ccgccaaatc tttaaagcca atgaccatca cccctcaatc accaatgcgc gaagtgctgg
     4321 aaatttatcc cggcgcacag cgggcgttgt tccgcaagta tcacatcggc ggatgcagca
     4381 gttgcgggtt tcagcccact gaaacgctgg cgcaggtttg cgcgcgcaac aacaacctga
     4441 acgtggatga agtgttgcag cacatccaga ccagccacgc agaagatgaa aagattttga
     4501 ttttaccgaa gcaattggcc gaattgcgcg cgaaaaatcc gtcgctcaaa ttgctcgacg
     4561 tgcgttcgcg cgaggaattt gaggccacgc acatcgaagg ttcgacgttg atgtcccagc
     4621 cgacggtgca ggaaatcatg gcgcattggc cgcgcacgga ggaattcgtg attgtggacc
     4681 ataccgggaa aatgggattg gatgccgcgg cgtattttgc gggacacggt tttgagcgca
     4741 tccgctgttt gcgcggcgga attgatgcct ggtcgcgcga agtggactcg aacgtgcggc
     4801 gataccaact ggcttagcaa tatgcgattt acgatatacg atttacgcgg gttggttgcc
     4861 agtggcgtat caatcgtaaa tcgtatatcg taaatagaat atgacagaag atttacagaa
     4921 gcgcgttcag caggcgttgc agaacccgca aaacatgggc gagatggccg gcgcggatag
     4981 tgttggcacg gtgggcaact ccgagtgcgg cgagatgttg cgggtgtggg tgaaattcaa
     5041 ggaggagaac gggaaaaagg tcattgaccg cgccacattt caatcgttcg gctgcgaaac
     5101 cgccattgcc gttgccagcc ttgcaactga attgattcgt ggcaaaaccg ctgatgaagc
     5161 cctggcaatg aaaaccgagg agctggccgg cgaactcggg ccgctgccgc ctatgaaaat
     5221 tcattgtgcg caactcgtcg aaggcgcttt gcgttccgcg cttgagccgg aaagcggcgc
     5281 gccgaaaccg gctgttgctg cacatgccca accggctgcc aattccaatc tgctggataa
     5341 tttcaccaag cccaaggaag gcggagtgcg cgtgaaattc ctggattagc atgccaaaag
     5401 tggcatctga gcgtttcacg cttaaaaatt tcccgaccaa agctcacgcg tgcgcatcag
     5461 gccaaaggca actccgaatg taaacccggt taagagagtt gcgacgacca gcgtttttgt
     5521 tgaagttgtg tatcttacaa atctccaacc gctgagacat ccaacgataa aaccgcaaac
     5581 gaatcggact tgaaatccta tccagtcgaa ctttttccgt ggttcttcct cgccttgaag
     5641 agtgcctgct attttccaca gcatccaggc gatcagcagg gaccaggaaa acaatccaat
     5701 taacagcact gaaagctgaa cgattttgcc gacatggaaa gtatttacag ccgacatgat
     5761 tgacaacact ggaaaataga aaattccgca gccagcaaaa atttttatac cgagcgcctg
     5821 cgaaatcaat cctttggagc tcaaaccaac ggctgcaaac atgaggacca tcggaataaa
     5881 ggccagaatt gcggccccaa ttttgattgt tagccgcata atcgataaac catcactcgc
     5941 ttcgttttgg tggaaggctc gccagctttt tttccggagc ttgtgaaaca ggagcagggg
     6001 gttgtttcgc ttcccaaaca atttttccgt caaggagagt caattgcacc tgggtatcgc
     6061 gaacatcatc cacagcacat ttcatgatgt cgcgatcaag cacgataagg tcggccaatt
     6121 tgccgatttc aagcgagcct ttcttttcct cttcgaaatg cagcttggca ttgttcatgg
     6181 tataaaactg aatggcctgt tcgcgagaca gttgttcttc cttgacgtaa atgccatcat
     6241 tctgcgccgt ctgccgcgcg agcgtgaccc acaagccgta ccatggattc caaaaattgg
     6301 ctgagtcgag cgaattcaat ccgctctgat gaccgctgcc ggctccgata agcaggccat
     6361 gatcgaacca acctttgaac gcttcgaaat tcctgagccg tttctcaccc agaagcttca
     6421 acatgccgga gccatcgtga taaagacaga tgggctgcat ttcggcccca acgcctaatt
     6481 tcacgcaacg attccaatcc tgctccgctt ggaagttcgc atgccgcagt aaaaaacgac
     6541 gctggctaat gtcggtcttg aactgcactt tttgataaca attgagcaac tggtcgagcc
     6601 cggcatcgcc cgtgcaatcc gccgtgattt gccatccgcg ttgggcggca gcggtgaaaa
     6661 atgaggtcaa cacatcgggg tcggttacaa gtttgccgcg ataggcgggc tcagtgattt
     6721 gaaaagccgg cccaatgccc caaggtgtgc gcaagtatgc ggtactgtcg agcaggtcgc
     6781 cgtcaacctg cgcgagcaac ggcccgatgc gtacccaatc atcacccgcg ccggtgggac
     6841 cgtattgcgg tttgtcggca ggcgtattgg tgagagcgtc caaccgtgcg gtggtttcct
     6901 cgattgtggc gcccggcgca acataacgcg tgcagttgat gcggacggtt agttcattgg
     6961 atgccgccat gtcacggaac agattgatga cctccggttc ggcgtcgctt tcgccgatgc
     7021 tggtgatgcc ttgctcgttg taaagcccgt ataaatgttt gagggcatcg cggtgttgct
     7081 gtgcggtggc cgtaacagcg gcaggaggaa gtttcaccac acttgaggcg tggcgaagca
     7141 atcctgtcgg ttttcgcgtg actggatcca gcacaatttc gccaccggat ggattggtgg
     7201 tggtcgtggt gagatgcaaa acttccaatg ccttggtgtt cacgactgcg agatcaccac
     7261 agttccaata aacaggattc ttgggagcga ccgcgtccaa ctcggccttg gtcggcaggc
     7321 ggcattcttt caaccgcgtg gggaaagcgc gttcgactac gatccacgcg ccagcgggtt
     7381 tttgcgcggc ttggtcatgg atgaaatcct tcgccctggc aatggaatcg aacatcggca
     7441 gcggcccatt caaggtgctc actgaagcct gatatgaatc caccaaattg tcgtacaaac
     7501 ccggcatgac cgtgtgttca tgaccatcta tgacccgagt ttcaggacct ttgaaacgca
     7561 atatttgttt gtccttgcca atcgcaacga tttggttccc gcgcactgcg atggcttggg
     7621 ctgtggtgaa atttgaattc accgtgagca ctttcgcatt acagatgatg acatcggccg
     7681 gtggcggatt ttccgcggcg gtgagtgtcc cgtgtgtggc gaaaaacagg acacttccca
     7741 atgccaaact attgaaaatg gtacacaatt tcataattca tctaaatatt gtctccggat
     7801 aacatccggt tgtgaaccga tacggctcgc ctgcaatcgg agcatgtttc aagccatgga
     7861 gatcggctgg tcggggccgt caactgccat ccttgcgaaa atcagtgagc ctgctaaagt
     7921 ccgacaacca tgaaccatta aaacaaaaaa gagaacctat gaaagacttc agagaggaat
     7981 tctcattggc aaatgcgaag aagaagctcg gcattctgtg ggagttcaag cagtttgcga
     8041 tgcgcgggaa tgtcgtggat ttggccgttg gcgtggtcat cggcgcggca ttcggaaaga
     8101 ttgtcaccgc gatggtcagc gacttcataa ctccgatcat tggcaagtta acatcaggcg
     8161 gcgctgattt aaaaaataaa ttcatcagtt tggagccaag taaaaccgag gggatcaaat
     8221 ccctggccga tgcgcagaaa gcaggagcag tcatcgcttg gggagatttc gtgacctgta
     8281 ttgtggattt cttgatagta gcattttgca tctttctcat tgtgaagctc ttcaatcgcc
     8341 tgaacatggc ttctgcggcg gttgcgcctc ctgccgaacc gacccccacg gaaaagcttc
     8401 tgggggaaat ccgcgacgag cttaagaaaa agccagtcta gaaatctctg cacacctggt
     8461 tcgatcgccg actcggaaga atatcgagaa tcttcatgtt tggcattgct gatgctgtct
     8521 aaaagccatg gtaattgcag cgattatcgt gggagccggg gtgtttgtcg aattattggc
     8581 aatcctcaaa gctcctctgg gttatcagga tgataccgga tttcatgtcg gcgcttccgg
     8641 cgctgaaaat gaagaaggcc agccttggct gaacccaagc tgaaagacga cgagaattta
     8701 cggttttctc cctcggttcc tcaggttagt tgcgtgttag taaacagttg gttataggtt
     8761 gtgtaaccaa aaccgctgac cggtccgtct ctgttttccg gcagagccgg gaaaccgatc
     8821 ggcagatgag tcctccgttt gggcaagcgt tggcgcgtca gcgccagagc gcggcttgaa
     8881 cgggggactc tgtttttcaa gggctgactg aaatgtagaa agtccgcggg caagtcaatg
     8941 atactcctgc accgtgcgca caccgtagcc gcttttcttg agggcggcga tgccaagcgc
     9001 ggcggccttg gctccgctca aggtcgtcat gatgggtgtt cccgtataaa ccgccgtggt
     9061 gcggatcttg atttcgtctg cgcgcggtgt ttggcctgcg ggagtattga tgacaagctg
     9121 tatttcccgg tttttcaaaa ggtcgagcgc attgggccgg ccttccgcaa gtttataaat
     9181 gcgctgcacc ttgaggcccg ccttttccag gactgccgcg gtgccgctgg tggaaatcaa
     9241 ttcaaaaccg agctttgcaa attcttgcgc aacatccgcg acttctgatt tgtgagcgtc
     9301 gctcacgctg atgaacaccc gcccgctcag cggtagcggc gcgttggcgg ccatctgcga
     9361 cttggcatac gcgatgccca aatcggtgtc caatcccatg acttcgccgg tggaacgcat
     9421 ttccggcgaa agcaaaatgt cctgtccatg aaaacgattg aaaggaaata ccgattcctt
     9481 cacggcccaa tattcagtcc agatttcctt ggtgaaattc aattcggtca atttttttcc
     9541 ggccatcacc ttggcggcca gcttggcaag cggcacaccg atggccttgc tcacgaacgg
     9601 cacggtgcgc gacgcgcgtg ggttcacttc gagcacatag acgacgtcgc ccttcacggc
     9661 gtattgcaca ttcatcaagc cgatgacctt caactcgcgc gccatggcgt gtgtgtattg
     9721 gcggatgatt tcgatgacct tttgcgaaag cgtgtgcggc ggcaagacca tcgcggcatc
     9781 gccggaatgg acacccgcgt attcaatgtg ttccaacatg ccgccgatga cgatggttcc
     9841 ctcatttgga tttgcaaaat ggcccacgtc cgcgatgcaa tccacgtcaa cctcggtcgc
     9901 atcttcgagg aatttgtcca cgagcaccgg gcgttccggc gaagcttcga ccgcgaaacg
     9961 catgtaatgc gtcaattccg aatccgaata aacgatctgc atcgcgcgac cgccgagcac
    10021 aaaggatggg cggacgagga ttggataacc gagcttgtgc gcggcttcca gcgcctcggc
    10081 ttcgttcgtc gcaagaccgt tgggcggttg gggaatgtcc agcttgcgca acatcgcggc
    10141 aaataatttg cggtcctcgg cgatctcaat gctttgcggc gaggtgccga tgatgttgac
    10201 gccatttttt tgcaaaccaa gcgcaaggtt caacggcgtc tgcccgccga actgcgcaat
    10261 cgcgccccaa cacttttcgc gctcgtaaat gtgcagcacg tcttcaagcg tgagcggctc
    10321 gaaaaaaagt ttgtcgctgg tgtcgtaatc ggtcgagaca gtttccgggt tggaattcac
    10381 catcaatgtt tcgaagccgt cttccttgag cgcaaaggcc gcgtgcacac agcaataatc
    10441 gaactctatg ccctgcccga tgcgattggg gccgccgcca agaatcatca ccttgcgcgt
    10501 ggtcgtcggc ttgatttcgt catcgccgca atcgtaggtg gaataataat aaggcgtgta
    10561 ggcctcgaac tccgcagcgc aggtgtccac aagacgatag ctcggaacga ggccgagttt
    10621 tttgcgttca gcgcggacgg catcttcaga cgtgcccgtg agatgagcga tctggcagtc
    10681 agaaaaaccg agtgattttg ctttggtcaa caattcgcga ttcatgcgta aagaaactcg
    10741 gcaaagtaag catcagcttt gccggactgg caagacacga attgccaaaa agaagataaa
    10801 ggcgcaaaaa aattttcaga accaacaaaa taatcttgcg ccttggctat ctcacggcgg
    10861 aggcggtggt gcgggcacag gaattgccgg cggaggaggc aaacccggag gcccgggcgg
    10921 ttgcacccat gcgcatcctg tcgtgagcac ccaactcaaa gcgagggatg cgacaagtaa
    10981 tttccggaac gttttcatac agcttccttt cgttcggtaa ccctggtggc cctggttcct
    11041 tcttgaacac aagatagcat acatccggca cctttgaaaa tgggctttga cggaattgca
    11101 agaagaggta tttgtgcaat acgtactgcg cagaaaaacc ggcgttcata tgaaaacaaa
    11161 gccaaccagt ctcacgccat acgtcgcgct gcttcgcgga atcaatgtcg gcggcaagaa
    11221 catgctgccg atggccgatt tggcgaaaat gttcgtcacc gcacgctgca cggatgtaag
    11281 gacttttatc cagagcggaa atgtgatctt caatgccaca ccggatttgg ccggccggat
    11341 tgctgaagta atctccgcgc aaatcgccag gaaatttgga tgcaagattc cagtggtgct
    11401 gcggtccgcg cggcagattg aagaaacggc tgtgaacaat ccgttcatca aagctggagc
    11461 accggaggaa atgctgcatg tcatgttcct cgccgacttg ccgaaatcgg acaaggttgc
    11521 tcaactcgat gccaaacggt cgccgccgga tgaattcatc gtgcgcgggc aggaaatttt
    11581 tctgaaactt cccaatggcg ctgcacgaac caaactgacg aacgcctatt tcgattcgaa
    11641 acttgcgacc atcagcactg gccggaactg gcgcactgtg ctcaagctgc tggagttggt
    11701 gaaagcgtag tcagaactcc acgcatttcg atttcaaaga gcaaatcaaa aatacagcta
    11761 tccgcgcggc ggcagcttct tctccacgcg aactttttcc agcgacacaa atttgggcaa
    11821 gccgccttcg gtggggacct tcatttcgcc ttccaccagc gggcgggcat attcgacgaa
    11881 tttttccgta ggcatgaaac catcttcgct gatccaatcc cgcggaagca gatgctcgac
    11941 gttcgcgatg tcgcccaatg gctgcaaacc ggtggtccac ttgtaaggct cgctttgcgt
    12001 gcgaacgatt ttcaccatga atccgctttg gccggcgata gcagcgcgca cggcagcttc
    12061 gccgcaagcg gcggcttcgg tggcatcggt tgcgctggcg taatgcgcag cggcgcgttg
    12121 ggcgtaaccg agcttcacgg tgcgcgtctt ggtgccaagc ttgccctgaa cgagttccgc
    12181 caatcggtcc gcggcgcccg aaagaaccgg atgaccgaaa gcatcgaggc gcgatttgtc
    12241 ggcggcgatt tcctcaccag cggcgttttt cacgccttca cccacaacca cgatgcaata
    12301 cttgtgctgc tcaatggttt ccttgacgcg cgcgaggaac gcggcttcat ccaacttgat
    12361 ttccggcagc aaaatgatat gcggcgcatc ggcaggcgaa cgcttcgcca aaactgtccc
    12421 agcagcgatc catccggcag agcggcccat cacctcgatc aaacagcaaa acccgccgtc
    12481 ggtcgccatg ctgcgcacgt cttcgccgac ttccatcacc gtggtggcgt tgtatttgat
    12541 gaccgaaccg taaccggggc aatggtcggt atggggcaaa tcgttgtcaa tcgtcttggg
    12601 aacgccgatg atgcgcatct cgtaaccgcg cgcgaccgcc tgttcatgga ttttgttcgc
    12661 ggtgtcctgc gaatcattgc cgcccgcata aaaaaagtag cggatgttgt gcgcctggaa
    12721 tacctcgaac aaacggtcca tgtccttggc ggcttgttcg gcctttttct tgaaatcgag
    12781 tttgtaacgg caggtgccga gcgcggcggc cggagtgtat ttcagccctt cgatggtgcg
    12841 ggccttttcc tcgttgatgt caatcaagtc ctcgttgagc agcccaagga tgccgttgag
    12901 cccgccgtaa atctcctcga tgcactcgtg cttgccggct tcctggatga cgccggccac
    12961 gctggcattg atgacagcgg tgggcccgcc cgattgagct accaataaat ttccgactaa
    13021 ttcagccata tattgcttcc gattaagacg acgcgatttc cgccgtcggt tggtttgaac
    13081 cgtaaaaagc gaatgcgaac cttgcccttt ggcaacattt ctgtaaaggg caattcgcag
    13141 actctgcctg ccatgaatgc cttccaccat ttcgtcttcc gagcaacaaa aaaattgcgg
    13201 aaacaagcag aacggcgcga caccacgcgc aacaagcggc agtcctctgc cgcttttccc
    13261 tgaagcgaca cacccaaacg aggatagcgg atggcaaaga cgtggcgcag gagcgttaat
    13321 acgatgagtt taccttcgaa aaaattaatt tttttatcag gaattaagcg gaaatttctc
    13381 cgcgacgatg gcggaaaatt cgtggatgaa ac
//
LOCUS       DASZMM010000370         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_722181, whole genome shotgun sequence.
ACCESSION   DASZMM010000370 DASZMM010000000
VERSION     DASZMM010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722181"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VG754_09030"
     CDS             <1..1131
                     /locus_tag="VG754_09030"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HWF19400.1"
                     /translation="LGAATDPNSPTSLYQLYANGLLPPNINSLQAFTNYVISTGYQLY
                     GPTGNTITQDAGSSATNAVKSRLAAQAASIASNPAFRNPGVPLTHLSDRRTAYTIAPT
                     LLWGIHTLTPEWTYSREHDYHSYGSAVNYSAALNEKNTVLNAGWSHDVDRVLDLAAGG
                     VWVGKVSDDFLIGVNQLLSPKSYLTVDLTYGQEYGYLSDQYRIALLDVPQDFTPGSQG
                     SPLPDTRPRRRTKEILYANYVQFIEPVQGSADVSYRFFHDSYGIFANTAELDWHQKLG
                     RYVVFTPGFRYYRQTAANFYYILVPADANGGPAVENFSSDYRLSEMETFTMSADFTFR
                     IQKHISVDISYMRYVMSGLDGMTSQSAYPSANVYSIGGRIWF"
     gene            1144..2142
                     /locus_tag="VG754_09035"
     CDS             1144..2142
                     /locus_tag="VG754_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWF19401.1"
                     /translation="MLETASNAFDAVYGRVRQSGRCATKDGYHQLSFQAMSTHCRVNF
                     LTPNTALAKQFQEEVLHWVSWFESHYSRFIPDSLIGRINAAAGLHWVEVDAETDALFN
                     LCQEMVFFTRGVFDPTALPLMRLWNWKNKPAVVPSDKAVAEARELVGWRKIQRRKGGI
                     FLPREGMCLDLGGIGKEYAVDRVLQMGLDRGITNLLVDFGQDVRVHGQPPERGCWHIG
                     LEDPLQPGKCWTGVAVNNHAVATSGDYLRHYVVNGRRYGHILDVRDGYPVNNGCLSVS
                     IIAPHCTMAGILSTSAFVLGAKEGLDLITVCPGVEGAITTETNRVHTRNFYAYATK"
     gene            2129..2635
                     /locus_tag="VG754_09040"
     CDS             2129..2635
                     /locus_tag="VG754_09040"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWF19402.1"
                     /translation="MRRNKKTLLMLAVSLGLAFAACADIKVGDSFPELAPFKLEGKLP
                     DAFKDKVVLVDFWASWCGPCGESFPAMEELQKKYGAQGFVIIAVNVDEKKSDMEDFLK
                     DHHVSFIVVRDAKQKLVDKAGIATMPSSFLIDQHGKVVFAHSGFHGSATKKQYEQEIE
                     SLLKNNAK"
     gene            2632..2856
                     /locus_tag="VG754_09045"
     CDS             2632..2856
                     /locus_tag="VG754_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HWF19403.1"
                     /translation="MKTKHLLLGLAVLAIAFGTTGCQGVKPWQRGTLADYTMRADRDP
                     LGDAQGDHIFFSREMASGGKGVGGGGCGCN"
     gene            2869..>3672
                     /locus_tag="VG754_09050"
     CDS             2869..>3672
                     /locus_tag="VG754_09050"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD N-terminal
                     domain-containing protein"
                     /protein_id="HWF19404.1"
                     /translation="MSTKRKIFGRILATHLVNKERSAAVSAGPAAAAQNNQDFYSLLR
                     LALRAQPRSIIYELACRTVLINAAARTASVVILSFILTSSLLAGEQQRSPFGIQVVPK
                     PDSSSHTVEVKFTIPSDCFLYSERLHFLTGDDDDVAPSKIPEPLTEMDKVSGKQKKVY
                     EKNFNVELPMASLADNRLMVKFQGCSNGSCFFPEKRTFVLNTNPASAKMFTEIMPAAT
                     PSIPSPADSDWAKQLNGFKVAARQSGYLPTKKFLSFLERAQSGNDSVDDP"
BASE COUNT          840 a          997 c         1007 g          828 t
ORIGIN      
        1 cttggcgcgg ccacagatcc gaacagcccg acctcgcttt accagcttta tgccaatggg
       61 ttgcttccgc ccaatatcaa ttcgttgcaa gcgttcacta attacgtcat cagcaccggg
      121 tatcaattgt acgggccaac gggcaatacc atcacccaag atgcaggttc aagtgccacg
      181 aacgcagtta aatcgcgttt ggcggcgcag gcagcctcga tagccagcaa cccggccttt
      241 cgcaacccag gggtgcccct cacacacttg agcgatcgga gaacggctta taccattgca
      301 ccgaccctcc tttggggaat tcacacattg acgcctgaat ggacctatag ccgtgagcat
      361 gattatcatt catacggcag cgcggtaaac tattcggctg cgctcaacga gaagaataca
      421 gttttgaacg cgggctggtc gcatgatgtg gatcgcgtgt tggacctggc tgctggcggc
      481 gtttgggtcg gaaaggtgag cgatgatttt ctcattggcg tgaaccagtt gctctcgccg
      541 aaatcttatt tgaccgtgga cctgacctac ggacaggaat acgggtattt aagtgatcag
      601 taccgaatag ccctgctcga cgtgccgcag gatttcacgc caggatcgca aggttctccg
      661 ttgcccgata cacgcccccg gcggcgaacg aaagagattt tatacgcgaa ctacgtgcaa
      721 tttatcgagc ctgtgcaagg cagcgcggat gttagctacc gtttttttca tgattcatac
      781 gggatttttg cgaacactgc cgagctggac tggcatcaaa agttgggtcg ttacgtggtg
      841 ttcacgccgg ggttcagata ttatcgtcaa actgcggcga atttttatta catattagtg
      901 cctgcggatg caaacggcgg gccagccgta gaaaattttt cgtcggatta tcggttgtcc
      961 gaaatggaga ctttcaccat gtcagcagat tttacctttc ggatacaaaa gcacatctct
     1021 gtggatatta gctacatgag atatgtcatg agcggtctgg atgggatgac gtcgcaaagc
     1081 gcctatccct ccgccaatgt ttatagtatt ggcggtcgca tctggttctg atataggcga
     1141 ataatgctcg aaacagcttc caacgcattc gacgcggtct atggccgcgt gcggcaatcg
     1201 ggccgttgcg cgaccaagga cggctatcac cagctttcct tccaggcgat gagcacccat
     1261 tgccgggtga atttcctcac gccgaacacc gcgctcgcga agcaatttca ggaagaggtc
     1321 ttgcattggg tgtcgtggtt cgaatcgcat tattcgcgtt tcattccgga cagcctcatc
     1381 ggccgtatca acgccgccgc gggtttgcat tgggtggaag tggatgcgga aaccgacgcg
     1441 ctcttcaatc tttgccagga gatggttttt ttcacccgcg gcgtgttcga ccccacagcg
     1501 ttgccgttga tgcgcctttg gaactggaaa aacaaaccgg cggtcgtgcc atcggacaaa
     1561 gcagtcgccg aagcgcgtga actggtcggc tggcgcaaaa tccagcggcg caaaggcggg
     1621 atatttctgc cgcgcgaagg gatgtgcctg gacttgggcg gcatcggcaa ggaatacgct
     1681 gtggaccgcg tgctgcaaat gggtttggat cgcggcatca cgaacctgct ggtggacttc
     1741 ggccaggacg tgcgcgtgca tggccaaccg cccgaacgcg gttgctggca catcgggctc
     1801 gaagacccgc tgcaaccggg caaatgctgg actggcgttg cggtcaacaa ccacgccgtc
     1861 gcgacttccg gcgattacct gcgccattat gtcgtcaatg gccgacgata cggacatatc
     1921 ctcgacgtgc gcgacggtta tccagtgaac aatggctgcc tctcggtatc aatcatcgcg
     1981 ccgcattgca ccatggcggg aattttgtcc acatcggctt ttgttctcgg cgcaaaggaa
     2041 ggattggatt tgatcaccgt ctgccccggc gtggaaggcg ccatcacgac tgagaccaat
     2101 cgcgttcaca cgcgcaattt ttatgcttat gcgacgaaat aaaaaaactt tgctgatgct
     2161 tgccgtcagt ttgggcctcg cgttcgcggc ctgcgccgac atcaaagtgg gcgattcatt
     2221 ccctgaactc gctccgttca agctcgaagg caagctgccc gatgcattca aggacaaggt
     2281 cgtgctcgtg gatttctggg cgtcatggtg cgggccatgc ggcgaatcgt tcccggcgat
     2341 ggaggaattg cagaagaaat atggcgctca aggcttcgtc atcattgcgg tgaacgtcga
     2401 tgaaaagaaa agcgacatgg aagactttct gaaggaccat cacgtttcct tcatcgtcgt
     2461 gcgcgacgcg aagcaaaagc tggtggacaa ggccggcatc gcgacgatgc ccagctcctt
     2521 cttgatcgat cagcatggca aggtggtttt tgcgcacagc ggatttcacg gaagcgcaac
     2581 caaaaaacaa tacgagcagg agatagaatc gctgctcaag aacaacgcga aatgaaaacc
     2641 aaacatcttc tccttggtct tgcggttttg gccattgcat tcggcaccac cggatgccaa
     2701 ggcgtcaaac catggcaacg cggcaccttg gcggattaca ctatgcgcgc cgatcgcgac
     2761 ccgctgggcg acgcacaagg tgaccatatc tttttctcac gcgaaatggc gtcgggcgga
     2821 aaaggcgtcg gcggcggagg gtgcggttgc aattaaggaa tttccggcgt gagcacgaag
     2881 cggaaaattt ttggaaggat acttgcaact catctcgtaa ataaagagcg gagcgcggct
     2941 gtgtccgcag gaccagccgc agcagctcag aacaatcagg atttctacag tttgctgcgg
     3001 ctggcgcttc gcgcacagcc gcgctccatt atttatgagt tagcttgtag aaccgttctc
     3061 atcaatgcgg cggctcgcac ggcatcagtt gtcattctct ctttcattct cacttcatct
     3121 ctactggccg gcgaacagca gcgcagcccg ttcggcattc aagtagtgcc caagcccgat
     3181 tcaagcagtc acaccgtgga agtgaaattc acgattccgt ccgactgttt tctttattcc
     3241 gaacgcctgc attttctcac cggagatgat gacgacgttg cgccgtccaa gattcccgag
     3301 ccattgacgg aaatggacaa ggtgagcggc aaacaaaaga aagtttacga aaagaatttc
     3361 aacgtcgaat tgccgatggc atccctcgcc gacaaccggc tcatggtgaa atttcaaggt
     3421 tgcagcaacg gctcctgttt tttcccggag aaacgcacct tcgtgctcaa caccaatccg
     3481 gcttcagcaa aaatgttcac agaaatcatg ccggccgcga cgccctccat tccgtcgcct
     3541 gcggactccg attgggcaaa gcaattgaac ggtttcaagg tcgccgcgcg ccagtccggt
     3601 tatctgccca caaaaaaatt tctttccttc ctggaacgcg cgcaatccgg caacgacagc
     3661 gtggacgatc cg
//
LOCUS       DASZMM010000371         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3706261, whole genome shotgun sequence.
ACCESSION   DASZMM010000371 DASZMM010000000
VERSION     DASZMM010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3706261"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VG754_09055"
     CDS             <1..1169
                     /locus_tag="VG754_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWF19405.1"
                     /translation="RVQGQVAREEELVIAPEFFHALRKHLPRITHVEIQLKPGRFHNE
                     VTRFRYDVTLHVDKAVHPEANIVWLDNAPESLTPGIITEALSQNGSGYAGIRRVPNAR
                     LVNENLALAWMSGSEGPETLGELRAAAQAERKVNAVDPENVLTLAREAGCAVDIIWTD
                     DADDTGAFDVIFRRSELPSPTISSRNISSRRKAWAEFANQPAKGRPDTKLVNQLRTHL
                     KQRLPAIMVPSAFVVLDAMPLTPNGKVDRRALPDANVTREEPAESFIAPRTSVEEALA
                     GIWREILGLDSVGVEDDFFKLGGHSLLATQLVSRLREVFKIELPLRSLFEAPTIAKLA
                     RQLVAHEAMPGIIERTARILNQLEAMSAGELEAALEQRKSAATTGVLTAEPANA"
     gene            1261..1482
                     /locus_tag="VG754_09060"
     CDS             1261..1482
                     /locus_tag="VG754_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19406.1"
                     /translation="MKALLQQVETGLYFHAPGEWTPEHQEARDFKSSVTARTYCLRHG
                     IEGVQIVLKFESEKYDIVLPSRQSGLHHE"
     gene            complement(1490..1840)
                     /locus_tag="VG754_09065"
     CDS             complement(1490..1840)
                     /locus_tag="VG754_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19407.1"
                     /translation="MSLLTAILKTVGSLVPDSMVDRIAREYFNHRFRSLGAMTTLQID
                     SAAKRATCDLELKGETQAVHVNIGRYELSTIGGKTFLEIHEVSTSREWLTMLAQQMVK
                     GRKFEVPEALTSVL"
     gene            2050..2706
                     /locus_tag="VG754_09070"
     CDS             2050..2706
                     /locus_tag="VG754_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWF19408.1"
                     /translation="MPQIFQRRTNTIARTSILAAVIFFILAGWVLYAAYWAPYTTLQG
                     VPLSQPVPFSHKHHVGGLGIDCRYCHTSVEKSAFAGLPPTETCMTCHSQLFNTSPMLA
                     PVRESLATDAPLRWNRVNFVPDFVFFNHSIHVNKGIGCSNCHGELDQMPLTWKDQTLY
                     MKFCLGCHDHPEQFIRPREQVFNLHWQPPPNQQAQGQKLVRDYHVNSVQLSDCSMCHR
                     "
     gene            2703..>3170
                     /locus_tag="VG754_09075"
     CDS             2703..>3170
                     /locus_tag="VG754_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HWF19409.1"
                     /translation="MKPYPQSSGHVDLSLVRERLAGARGQKYWRSLEEVAQTPEFKEF
                     LHREFPENATEWTDDMSRRNFLKLMGASLALAGMTGCTRRPIQKIVPYVRQPEDIVPG
                     KPLFFATAMEHDGFATGLLVESHEGHPTKIEGNPDHPASLGATSVWDQAAILDL"
BASE COUNT          723 a          820 c          933 g          694 t
ORIGIN      
        1 agcgagtcca gggacaagtg gcgcgcgagg aggagctggt catcgcgccg gaatttttcc
       61 acgcgcttcg gaaacatctg ccgcgcatca cgcatgttga aatccagctc aagcccggtc
      121 gtttccacaa cgaagtgacg cgttttcgtt atgatgtcac gctgcacgtt gataaagccg
      181 tccacccgga agcaaacata gtctggttgg acaacgcccc tgagagttta acgcccggca
      241 tcattaccga ggcattgtcc caaaatggtt ccggttatgc aggcattcgg cgcgtgccaa
      301 atgcgcggct tgtaaacgaa aacctcgctt tggcatggat gtctggcagt gaaggacctg
      361 aaactttagg tgaattgcgc gctgctgcgc aggccgaacg gaaagttaat gccgttgatc
      421 ccgaaaatgt tctcacgctg gcgcgcgaag cgggttgtgc cgttgacatt atttggacgg
      481 acgacgcgga tgacaccggg gcgttcgacg ttattttccg gcgttcggaa ttgccatcac
      541 cgaccatttc ctcacggaac atttcgtcac ggcgcaaggc ttgggcggaa tttgcgaacc
      601 agcctgccaa aggccggcct gacacaaagc ttgtcaatca attgcgcact catctgaaac
      661 aaagattgcc ggcaatcatg gtgccatcgg cgtttgtcgt gctggatgcg atgccgctga
      721 cgcccaatgg caaggtggac cgtcgtgcgt tgccggatgc aaacgtcacg cgcgaagaac
      781 cagcggaaag tttcattgct ccgagaacat cggtcgaaga ggcgttggcg gggatttggc
      841 gtgaaattct cggcttggac agcgttggcg tggaggatga ttttttcaaa ttgggcggac
      901 attcgctgct cgcaacgcaa ctggtttcgc gtttgcgcga ggtgttcaag atcgaactgc
      961 cgttgcgcag cttgttcgag gcgccaacga ttgccaagct ggcgcgtcaa ctcgtcgcgc
     1021 acgaagccat gccgggaatc atagaacgaa cagcgcgcat cttaaaccag ttggaggcaa
     1081 tgtccgcagg ggaattggag gcggcgcttg agcaacgcaa aagtgccgca acaaccgggg
     1141 ttctcacagc cgaacccgcc aacgcgtgag cggatgcggg atgaactgcg gtgattcacg
     1201 caaaatacgg ccataacaag gagccgaaat tctttggcaa gcgaggttgg ggtaatctaa
     1261 atgaaggctt tattgcaaca ggtggaaacc ggactttatt ttcacgcgcc cggcgaatgg
     1321 acgccggagc atcaggaggc ccgcgatttt aaatcgtccg tgaccgcgcg cacttattgt
     1381 ctcaggcacg gcatcgaggg cgtgcaaatc gttttgaaat tcgaatcgga gaaatatgac
     1441 atcgtgctgc cctcgcggca aagtggattg catcacgagt aaactgactt taaagcacgc
     1501 tggtcagggc ttctggcact tcaaactttc tgcctttcac catttgctgc gcaagcatgg
     1561 tgagccattc gcgcgaggtg gagacttcgt gtatctcgag aaacgttttt ccgccgatgg
     1621 tggaaagttc gtatcggccg atgttcacat gcacagcctg ggtttcgccc ttgagttcca
     1681 gatcgcaagt cgcgcgtttg gcggctgaat caatttgcaa agtcgtcatg gctcctaatg
     1741 aacggaaacg atggttaaaa tattcgcgcg caatccggtc caccatcgag tctggcacca
     1801 gggaaccaac ggtcttcaaa atggctgtca gcagactcat tgacgggcgt tgcgcaattt
     1861 gttgaagaga aggtggacta gttttaacgg catggcaaga aggacggatg cgcgagagtg
     1921 tcgcaaaaat ccgcgatcaa aattccgcta gggtcttcgc cctatttacc gggatggtgc
     1981 tcgcggctaa ttttgcatcc gcacaacggg tgactgaaaa tccggacggg agcgggcgta
     2041 aggaattgga tgcctcagat ttttcagcgg cggaccaaca cgattgcccg gaccagcatt
     2101 ctggccgcgg tgattttttt tattctcgcg gggtgggtgt tatacgccgc gtattgggcg
     2161 ccttatacaa ctttgcaggg agtgccgctg tcgcagccgg tgccgttcag ccataaacat
     2221 catgtcgggg gacttgggat tgattgtcgc tactgccata cgtcggtgga aaaatcggca
     2281 tttgcgggat tgccgccaac ggagacttgc atgacctgcc attcacaact gttcaacacc
     2341 tcgccgatgc ttgctccggt gcgcgaaagt ctcgccacgg acgcgccgtt gcgatggaac
     2401 cgcgtgaact tcgtgccgga tttcgtgttc ttcaaccaca gcatccatgt gaacaagggc
     2461 atcggctgtt ccaattgcca cggcgaactg gaccagatgc cgctgacgtg gaaggaccag
     2521 acgttgtaca tgaaattttg tctcggttgc catgaccacc cggaacaatt catccggccg
     2581 cgcgagcagg tgttcaatct ccattggcaa ccgcctccca accagcaggc gcaaggacaa
     2641 aaactcgtcc gggattatca cgtgaactcc gttcaattga gcgattgcag catgtgtcac
     2701 cgatgaagcc ttatcctcaa tcgtcgggcc atgtggatct gtcgttggtg cgcgaaagac
     2761 tcgcgggcgc gcgggggcag aaatactggc gcagcttgga agaagtggca caaacgccgg
     2821 aattcaagga gttccttcac cgcgaatttc ctgaaaacgc cactgaatgg actgatgaca
     2881 tgagccggcg gaattttttg aagttgatgg gagcatcttt ggcgctggcg ggcatgacgg
     2941 gttgcacgcg gcggccaatc cagaaaattg tgccttacgt ccggcagccg gaagacatcg
     3001 tgccgggcaa gccgctgttt ttcgcgacag cgatggagca cgacggattt gccacgggac
     3061 tgcttgtcga aagtcacgaa gggcatccca ccaaaatcga aggcaatccc gatcatccgg
     3121 cgagtttggg cgcaacgagc gtttgggacc aggcggcgat tttggacctt
//
LOCUS       DASZMM010000372         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1533863, whole genome shotgun sequence.
ACCESSION   DASZMM010000372 DASZMM010000000
VERSION     DASZMM010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533863"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1169)
                     /locus_tag="VG754_09080"
     CDS             complement(72..1169)
                     /locus_tag="VG754_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF19410.1"
                     /translation="MKPISFGLRGSFIIISLLFLICAAHRLDAADYPAPEQGDFVIRD
                     FHFQSGQTLPELRIHYRTLGAPRKNEQGIVTNAVVILHGTTGQGGNFLRAEFAGELFG
                     PGQLLDAARFFIILPDGIGHGQSSKPSDGLHARFPNYGYRDMVEAQYRLVTDGLKVNH
                     LRLVMGTSMGGMHTWLWGEIHPDFMDALMPLASLPTQISGRNRVWRRMIIDAIRNDPD
                     WQNGEYKTQPHGLAVADEVLYFMSSNPIQRYKDVPTLKQADEALDRSVANAMKTMDAN
                     DTLYALRASEDYDPGPALEKITVPLLAINSADDLINPPELGIIEREMKRVPHGQFVMI
                     PLSDKTRGHGTHTLASIWKEHLEDLLAKTSK"
     gene            1276..3435
                     /gene="ppk1"
                     /locus_tag="VG754_09085"
     CDS             1276..3435
                     /gene="ppk1"
                     /locus_tag="VG754_09085"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HWF19411.1"
                     /translation="MGNAAQAYGPDKFINRELSWLEFNQRVLDEALDPQNPLLERIKF
                     FCITSSNLDEFFEVRVAGLKQQIESDVVERSIDGLTASETFRAVTQRVRRMVEQQYES
                     WRTDLLPALAQNGIRFLQFDELSKTDHEWVEEYYRAEVRPVLTPLALDPAHPFPQLLN
                     KSLNIIVRLEMPHGGEMLRHLAVVQVPRVLPRLVRLPRKDGRMDYIFLGNLIGHYLAD
                     LFPGTRILGYWNFRVTRNSELYIDEEETANLLKAVENELHNRRKGDAVRLEVEINCPE
                     DLRNALLNTLKLGEEDLFVIDGPLNPTRLMAIYEGDHSPELRDPPFVAPIAAALRDRQ
                     DIFEAIREHDILLHHPYETFDSVVKFLEQSAADPRVLAIKQTLYRTGGDLRIIGALMN
                     AVQNGKQVTAVVELRARFDEANNIQWARQLEEAGVHVVYGLVGYKIHSKILLVVRRDQ
                     DRIRRYVHLATGNYNPTTARLYTDTGLLSCRSDLGEDATNLFNLLTGISQFQGMRKLL
                     VAPFELHDRMLALIRREAANARKGLPARIIAKMNALVDRQIIEALYDASQAGVRIDLI
                     VRGICCLRPQMPGVSENITVRSVVDRFLEHSRIFYFENACQPEIFVGSADWMPRNFYR
                     RIEVIFPVADGNLRERIASELLALPLQDNVKARLMQADGTYHLAKAKHGETAHRSQLE
                     FMRRSQQPSAATNGEHSKSRYPKVKLRPKPAIKSKPG"
     gene            complement(3416..>5186)
                     /locus_tag="VG754_09090"
     CDS             complement(3416..>5186)
                     /locus_tag="VG754_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWF19412.1"
                     /translation="HKSSDSLRDFTTDMRMLLLSGMALVIGAMSALVAYALVWLIAVI
                     TNLAFFHTFSSAPNTPQEAHLGLWVILVPVVGSLVIGLMARYGSPKIRGHGIPEALEA
                     ILIGRSRMEPKVAVLKPISSAISIGTGGPFGAEGPIIMTGGAIGSLFAQMFHLSSAER
                     KTLLVAGAAGGMAAIFSTPVAAVLLAVELLLFEWKPRSFIPVAVSAVVASVLRVPLLG
                     AGPIFPIIPHGAMSAGELGVALVVGIAAGFGSGLLTMLVYALEDLFHKLPVHWMWWPA
                     IGAVFVGLGGYFDPRVLGVGYGLIHSMLRGEMIGMALLGLLVGKALVWSIALGSGTSG
                     GVLAPLLIMGGALGALMSKLIGVGDASLWAMVGMAAIMGGTMRSPLTGMVFLLELTHD
                     LNALPAVMVGSVAALGVTVLLLRRSILTEKIARRGLHITREYSVDVFNLLRVGEAMDT
                     NAPIVRANMPVTEFSQLIAQGHPELSRRQGTLIVDEEGFLSGIITRGDVVRALQNKSA
                     PEMTVSQAGTQNLIVAYPDELLHDAIKKMLDRNVGRLPVVERKNPRHIIGYLGRGNVM
                     AARARSIEEESLRDRGLLNPAST"
BASE COUNT         1155 a         1563 c         1418 g         1050 t
ORIGIN      
        1 cggtcccgta aatgagcatt actcatcgtt cggcccgatt ggcaaattta ttttcgatgg
       61 cgacgggtaa ttcatttgct tgtctttgcc agcaaatctt cgaggtgctc tttccaaatc
      121 gaggccaatg tatgcgttcc gtggccgcgc gttttatcac ttaacggaat catcacaaat
      181 tgaccgtgtg gcacgcgttt catttcgcgc tcgatgatgc cgagttcggg cggattgatg
      241 aggtcatcgg cggaattgat ggcgagaagt ggcacggtga ttttttcgag cgcgggaccg
      301 ggatcataat cttccgaggc acgtaaggcg taaagcgtgt cgttggcgtc catggttttc
      361 atggcgttcg ccacgctgcg gtcaagcgct tcgtccgcct gtttcagcgt gggcacgtcc
      421 ttgtagcgtt ggatgggatt gctgctcatg aaataaagca cttcgtcggc aaccgccaat
      481 ccgtgcggct gggtcttgta ttcgccgttc tgccaatcag ggtcgttgcg gatggcatca
      541 atgatcatcc gacgccagac gcggttgcgt ccggaaattt gcgtgggaag gctggcgagc
      601 ggcatgagcg catccatgaa atcggggtgg atttcacccc aaagccacgt gtgcatcccg
      661 cccatcgaag tgcccatcac gagccgcaga tgattgactt tgaggccatc ggtcacgagc
      721 cgatattgcg cttcgaccat gtcgcggtag ccataattgg gaaaccgcgc gtgaaggccg
      781 tcactcggct tgctcgattg gccgtgcccg atgccatctg gaagaatgat gaaaaatcgc
      841 gcggcatcga gtaactggcc gggaccgaaa agttcgccgg caaattcagc gcgaagaaaa
      901 ttgccgcctt ggccggtggt gccatgaagg atcactaccg cgtttgtgac gatgccttgc
      961 tcattctttc gcggcgcgcc gagcgtgcgg taatgaatgc gcaattccgg cagcgtttgt
     1021 cccgattgaa aatgaaaatc acggatgaca aaatctccct gttcaggcgc gggataatca
     1081 gcggcgtcga gccggtgagc ggcgcaaatc aggaacaaca aagagatgat gatgaaactt
     1141 ccccgcaggc cgaatgaaat cggtttcatc aatccagaat aacaaatcgg taatgtttga
     1201 aaagcatcac catcgtcatt ttgtcacaga cttgtcactc gcttttaact tgtttacggt
     1261 tatgatggtt tctttatggg aaatgcagcc caagcctatg gccctgacaa gttcatcaat
     1321 cgtgaactga gctggctgga attcaaccag cgtgtgctcg acgaggcgct tgatccgcaa
     1381 aacccgctcc tggaacgcat caaatttttc tgcatcacca gttcgaacct cgatgagttt
     1441 tttgaagtcc gcgtcgccgg actcaagcag cagattgaaa gcgacgtcgt cgagcgcagc
     1501 attgacgggt tgacggcgtc cgagacgttt cgcgcggtga cgcaacgcgt cagacggatg
     1561 gtcgagcagc aatacgaatc atggcgcacc gacctgcttc ccgcgctcgc ccaaaacggc
     1621 atccggttcc ttcaatttga cgagttgagc aagaccgacc acgagtgggt cgaggaatat
     1681 tatcgcgcgg aggtgcgtcc cgtgttgacg ccgttggcgc tggatccggc gcatccgttt
     1741 ccgcaactgc tcaacaaatc gctgaacatc atcgtccgcc ttgaaatgcc gcacggcggc
     1801 gaaatgcttc ggcaccttgc cgtggtgcaa gtaccgcgcg tcctgccgcg cctcgtcaga
     1861 ttgccgcgca aagatggccg catggattac atttttcttg gcaatctcat cggacattat
     1921 ctcgccgatc tttttcccgg cacgcgcatc ctgggttatt ggaattttcg cgtgacgcgc
     1981 aacagcgagt tgtacatcga cgaggaggaa acggcaaatt tgttgaaggc cgtcgagaac
     2041 gaattgcaca accggcgcaa gggtgacgct gtgcggctcg aagtggaaat aaattgcccg
     2101 gaggatttgc gcaacgcgct gctcaacacg ctcaagctgg gcgaggaaga tttgttcgtc
     2161 attgatggcc cgctgaatcc tacccggctg atggccattt atgaaggcga tcattcgccc
     2221 gaattacgcg atccgccgtt cgtcgcgccc atcgctgcgg cgttgcgcga ccggcaggat
     2281 atttttgaag ccatccgcga gcacgatatt ttgctgcacc atccttacga gacattcgac
     2341 agcgtggtga aatttttgga gcaatccgca gcggaccctc gcgtgctggc catcaagcag
     2401 acgctttacc gcaccggcgg cgatttgcgc atcatcggcg cgctcatgaa cgccgtgcag
     2461 aacggcaaac aggtgacggc ggtggtggaa ttgcgcgcgc gcttcgatga ggccaataac
     2521 atccagtggg cgcggcaatt ggaagaggcc ggcgtgcacg tggtgtacgg cctcgtcggc
     2581 tacaaaattc actccaagat tctgctcgtc gtgcggcgcg atcaggaccg catccgccgt
     2641 tatgttcatc tcgccaccgg caattataat cccaccaccg cgcggcttta taccgatacc
     2701 ggcctcttaa gttgccgctc cgacctcggc gaagatgcga caaacctgtt caatttgctc
     2761 accggcattt cgcagtttca agggatgcga aaattgctcg tcgcgccgtt cgagttgcac
     2821 gaccggatgc tggcgctcat ccggcgcgag gccgccaatg cgcggaaagg tttgccggcg
     2881 cggatcatcg cgaaaatgaa cgcgcttgtg gaccggcaaa tcatcgaggc gctgtatgat
     2941 gcctcgcagg ccggcgtgcg gatcgacctg attgtgcgcg gcatctgctg tttgcgtccg
     3001 cagatgccgg gcgtgagcga gaacatcacc gtgcgcagcg tcgtggaccg ttttttggag
     3061 cacagccgga ttttttattt tgagaacgcg tgccagccgg aaatttttgt cggcagcgcg
     3121 gactggatgc cgcgcaattt ttaccggcgc atcgaggtga tttttcccgt cgcagacggc
     3181 aatttgcgcg aacgcatcgc cagcgaattg cttgccctgc cattgcagga caacgtcaag
     3241 gcgcggctca tgcaggcgga tgggacatat cacctcgcaa aggcaaagca cggcgaaacg
     3301 gcccatcgca gtcagttgga attcatgcgt cgctctcaac agcccagtgc agcgacaaac
     3361 ggcgagcatt caaagtcgcg ctatcccaag gtcaaacttc gccccaaacc cgccatcaag
     3421 tcgaagccgg gttgagtagt ccccggtcgc gcaacgattc ttcctcgatg ctgcgcgcgc
     3481 gcgccgccat cacgttcccg cgtccaagat aaccaatgat gtgacgcgga tttttgcgct
     3541 cgacaacggg cagacgccca acattccggt ccaacatttt cttgatggcg tcgtgcagca
     3601 attcatcggg ataagccaca attaaatttt gcgtgccagc ctgcgacaca gtcatttcag
     3661 gcgccgattt attttgcaac gcgcgcacga catcaccgcg cgtgatgatg cccgacaaaa
     3721 aaccttcttc atccacgatc aaggttccct ggcgtcgcga caattccgga tgtccttgcg
     3781 caatcagttg tgaaaactcg gtcacaggca tgttcgcgcg gacgatgggc gcattggtgt
     3841 ccatcgcttc tccgacgcgc aacaggttga atacatccac gctgtactcg cgcgtgatgt
     3901 gcagcccgcg tcgcgcgatt ttttccgtca agattgaacg gcgcaaaagc aaaaccgtta
     3961 cgcccagggc cgccaccgag ccgaccatca ccgccggcag cgcgttcaag tcatgcgtca
     4021 attccagcaa aaacaccatg ccggtcaacg gcgagcgcat tgtcccgccc atgatcgccg
     4081 ccatccccac cattgcccac aagctggcat cgccaacgcc aatcagcttt gacatcagcg
     4141 cccccagcgc gccgcccatg atcagcaacg gcgcaagcac gccgcccgaa gttcccgacc
     4201 ccagcgcgat ggaccacacc agcgccttgc ccacgagcaa tcccagcagc gccatgccga
     4261 tcatttcgcc gcgcaacatc gaatgaatca acccataacc gacacccagc acgcgtggat
     4321 caaaatatcc gccgagtccc acgaacaccg cgccgatggc cggccaccac atccaatgga
     4381 ccggcagttt gtgaaataaa tcctccagcg cataaaccag catcgtcagc aatcccgagc
     4441 caaatcccgc cgcgatcccc accaccagcg ccactcccag ttctcccgcg ctcatcgccc
     4501 cgtgcgggat gattggaaaa atcggccccg ctcccaacaa cggaactcgc aagaccgaag
     4561 ccaccaccgc ggaaaccgcg accggaatga aactgcgcgg cttccattcg aacaaaagca
     4621 gttccaccgc cagcaacacc gccgccaccg gcgtggaaaa aatcgccgcc attccgcctg
     4681 ccgcgccggc gaccagcaat gtttttcgct ccgccgagga aagatgaaac atttgggcaa
     4741 acaacgaccc aatcgcgccg cccgtcatga tgatcggccc ttccgcgcca aacggcccgc
     4801 ccgtgccgat tgaaatggcc gaagaaatcg gcttgagcac cgcgactttt ggctccatcc
     4861 ggctccggcc aatgagaatc gcctccagcg cttccgggat gccgtgcccg cgaatctttg
     4921 gcgacccata tcgggccatc aagccgatga ccagcgaacc caccaccggg accagaatca
     4981 cccacagacc gagatgcgcc tcctgcggcg tgttgggcgc ggaggaaaaa gtgtggaaaa
     5041 aagcgaggtt ggtgatcacc gcaatcagcc agaccaacgc ataagccacc aacgcgctca
     5101 tcgcgccgat caccagcgcc atgcctgaaa gcagcagcat gcgcatgtcc gtggtgaaat
     5161 cccgcaacga atctgaactc ttgtgg
//
LOCUS       DASZMM010000373         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4545122, whole genome shotgun sequence.
ACCESSION   DASZMM010000373 DASZMM010000000
VERSION     DASZMM010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4545122"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VG754_09095"
     CDS             <1..582
                     /locus_tag="VG754_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19413.1"
                     /translation="RNLYTSPVHFRSPSYREFESTASEQPDSRNPLQAGDQMGPFTVL
                     YPAPACKTAFPKASDNALVLRAEIYGTRFLLLSDLGHLGQNALLNSAETNSANRNFLQ
                     ADIVIAGLPGFEDDEPLSDALLHAIQPCAIIIADTRQPANKRAGAKLKQRLALHHIPV
                     FYTSITDGITLTIRPREWELRAMDGSSISSASR"
     gene            complement(589..1674)
                     /locus_tag="VG754_09100"
     CDS             complement(589..1674)
                     /locus_tag="VG754_09100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19414.1"
                     /translation="MEHQTSSSSPLISFIVPAHNEEACLGRTLQAIQESARALGLRYE
                     ILVANDASTDATAEIARQNNARVVNVNHRQIAATRNSGARAANGERLFFIDADTIANQ
                     RTVAAALRAMDKGAVGGGAPTWVAMSEKIPLYGRILFLLALLAPKIVGFAGGAFMFCT
                     REAFRATGGFNERLYCGEENWFALALKREGRFAGLCQPALTSGRRLRKTSGLQVVATF
                     SRLIFFPGKMKQRSSVEKVWYDSNRKDDDVMPKSWAARISNGITLLIVAVALTNPIWG
                     HLPWSWTPLSTPLGIMRLFTGVFLCHVAMLVFWPVALALLINFLRQKRFTGVIQSALL
                     IAFFGLNAWEATSVVIRTWSHIYHSLA"
     gene            2034..2390
                     /locus_tag="VG754_09105"
     CDS             2034..2390
                     /locus_tag="VG754_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF19415.1"
                     /translation="MSDSRKKTHCTARHLSRIFHELAGMSFRDKRAELRLARARELLA
                     TTNSKVVDVALESGYKSLSLFNLMFARRFGMSPGKWRKQHARQSDERRRIAKKIKLPA
                     QATIVAETVLHAAMLS"
     gene            complement(2400..3362)
                     /locus_tag="VG754_09110"
     CDS             complement(2400..3362)
                     /locus_tag="VG754_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HWF19416.1"
                     /translation="MITETSTAVEEAWPALPLAEWKGTCATLHMWSQIVGKVRLELSP
                     LMNHWWEVPFYLTARGLTTSPIPYGTGVFEMRFDFIDHRLNIETSWGENASIHLVPRS
                     VAAFYHEFMNTLRSLGIEVQIWPMPVEVPNPIRFDQDEIHGSYDADHVHAFWRVLITA
                     DTILKEFRARFIGKDSPVHFFWGSFDLAVSRFSGRDAPERQGVDRVTREAYSHEVSSA
                     GFWPGSGEVKDPAFYSYTLPEPAGFRDQPICPGKAFYHPRLHEFILMYDDLRASESPK
                     EDLLEFLQTTYEAGATLGNWNRAPLERSAETVITPGYATHCLLR"
BASE COUNT          874 a         1101 c          905 g          781 t
ORIGIN      
        1 cgcaatctct acacaagtcc cgtccatttc cgctcgccaa gctatcgcga atttgaatcc
       61 acggcatccg aacaacccga ttcgcgaaat ccactgcaag ccggcgacca aatgggcccg
      121 ttcaccgttc tttatccagc acccgcgtgc aaaaccgcgt ttcccaaagc cagcgacaac
      181 gcgctcgtcc ttcgtgctga aatttatggc acccggttct tgctcctttc cgacctcggc
      241 catctcggcc aaaacgcgct cctgaactct gccgaaacca acagcgcaaa ccgaaatttc
      301 ctccaagccg acatcgtcat cgccggtctt cctggtttcg aagacgacga accacttagc
      361 gatgccttgc tccatgccat ccaaccctgc gccatcatta ttgccgacac gcgccagccc
      421 gccaacaaac gagccggcgc aaaactcaaa caacgtctcg cgctgcatca tattccggtg
      481 ttttacacca gcatcacgga tgggatcaca ttgaccattc gtccccgcga atgggaattg
      541 cgcgcgatgg atgggagttc gatttcgagc gcgagccgtt aaagccattc acgccagcga
      601 atgataaatg tggctccaag ttcggatcac cacgctggtt gcttcccatg cgttcaaacc
      661 gaaaaaggcg atcaatagcg cggattgtat tacgcccgtg aaacgtttct gtcgcagaaa
      721 gttgatcaac aaggcgagcg ccaccggcca aaaaaccagc attgcgacat ggcaaaggaa
      781 cacgcccgtg aacaaccgca taattcccag cggtgtggaa agcggcgtcc acgaccatgg
      841 cagatggccc catatcgggt ttgtcagcgc caccgccacg atcaaaagag tgatgccgtt
      901 ggaaatcctc gccgcccatg atttcggcat cacatcatca tcctttcggt ttgaatcgta
      961 ccaaactttt tcaaccgatg aacgctgctt cattttgccc ggaaaaaaga ttaaccggct
     1021 aaaagtcgcc actacctgca atccggatgt tttgcgaaga cggcgtccgg acgtcaacgc
     1081 cggttgacat aatccggcaa accgcccttc gcgtttcaag gccagtgcga accaattctc
     1141 ctcgccgcaa taaagccgtt cgttaaatcc gccggtcgcc cgaaacgctt cgcgcgtgca
     1201 aaacatgaaa gcgcccccag cgaacccgac aatctttgga gccaatagcg cgagaagaaa
     1261 caagatgcgc ccgtaaagcg ggattttttc gctcatcgct acccatgttg gtgcgccgcc
     1321 gccaacagcg cccttgtcca tcgcgcgcaa cgcggcggca acagtgcgtt gattggcaat
     1381 cgtatccgca tctatgaaaa acaaccgctc accgttggcg gcgcgcgcgc cggaatttcg
     1441 ggtggctgcg atctgacgat ggttcacatt caccacgcgc gcgttgtttt gccgcgcgat
     1501 ttccgccgtc gcatccgtgg aagcatcatt cgccacgagg atttcgtaac gcagaccgag
     1561 agcgcgtgcc gattcttgga ttgcctgcag cgttcggcca aggcacgcct cctcattatg
     1621 cgcggggaca ataaaggaaa ttaacggaga tgacgacgat gtttgatgtt ccatgcgttt
     1681 gacaaataca gactacatgt gtagttatgt ttgtcaagcg ggattgaaca ccatttaaaa
     1741 cctctccgag gtcgcgagaa gatagcatta tgctagggca gccgaccaac tatctgacat
     1801 ctctctccat tacaatttcg tcagaggtca ttcctacaaa ccgatagcct tccttggtca
     1861 atccattcat tgcaatgttc atgtaaaatg cacggtcatc agcccgttgc ggtaccgcaa
     1921 ccttgcgcaa ctgtgctccg ataaattcca cctgacctcc aggcctgatg atgtgcgtgt
     1981 tatcacgtcc agcccaccga atcgtcacat attcgtgagt tcgaggtatt gccgtgtcgg
     2041 actcgcgcaa aaaaacgcat tgcaccgcac ggcatttgag ccggattttc cacgaacttg
     2101 cgggcatgtc ctttcgcgat aaacgcgccg aactccgcct cgcccgcgcg cgcgaactgc
     2161 tcgccaccac caattccaag gtcgtggatg tcgccttgga aagcggctac aaatccctca
     2221 gcctgttcaa cctcatgttc gcgcgccgtt ttggcatgag cccgggcaag tggcgcaagc
     2281 aacatgcgcg gcagtcagat gagagaaggc gcatcgcaaa aaaaatcaaa cttcccgcgc
     2341 aggcaacaat tgtcgccgag accgtcctcc atgcggcgat gttgtcgtga cgctaaagat
     2401 caccgcagca ggcaatgcgt tgcgtatcca ggagtgatga ccgtctcggc gcttcgttcc
     2461 agcggcgcac gattccaatt tccgagcgtc gctccggctt cgtaggtggt ttgcaaaaac
     2521 tcgagcaggt cttccttcgg cgattcggaa gcgcgaaggt catcgtacat caatatgaac
     2581 tcgtgcaacc gcgggtggta gaatgccttt cccgggcaaa tgggctgatc acgaaaaccg
     2641 gcaggttcgg gaagggtgta ggaatagaac gccgggtctt tgacctcgcc gctgcccggc
     2701 caaaatcctg cgctgctgac ctcgtgcgag tacgcttcac gggtgacgcg atcaactccc
     2761 tgtcgctcgg gcgcgtctcg accggaaaag cgcgacacgg ccaaatcaaa gctcccccag
     2821 aaaaaatgca ccgggctgtc cttgccgatg aaccgcgcgc gaaattcctt caaaatagtg
     2881 tccgccgtga tcagaacccg ccagaaagcg tggacatggt ccgcgtcata actaccatgg
     2941 atctcatctt ggtcaaagcg gatgggattc gggacttcga ccggcatcgg ccaaatttgc
     3001 acttcaatgc caagcgagcg cagcgtgttc atgaattcat gatagaacgc ggccaccgaa
     3061 cggggaacga gatggatgct ggcattttcg ccccaactcg tttcgatatt cagccgatga
     3121 tcaatgaaat cgaatcgcat ctcgaacacg ccggtgccat aaggaatggg cgaagtggtc
     3181 aatccgcgcg cggtgagata aaacgggact tcccaccagt ggttcatgag cgggctgagt
     3241 tccagacgga ctttgccaac aatttgactc cacatatgga gcgtggcgca ggtgcccttc
     3301 cattcagcca acggcagcgc cggccaagcc tcctccacag ccgtggacgt ttcagttatc
     3361 atgttacttc tccttgcatg tcgtcgaacg cgagcccggc cgaaaaagga aacgcgatgc
     3421 aattttattc aaccacggat cccccgcccc gacgttaata aaaactccgc tcaatgagag
     3481 ctttcgtcaa tcagttgctt tcgctgtggg cgcacaattt ccaaagttta aagttttcaa
     3541 atttgagtat tgctcgccct caccccatcc ctctcccccg gggagcgggt tgccttcagg
     3601 atcatttatc atcccggttg cattctccgt gtatcagcaa ttcctcaaat agaaactgaa
     3661 t
//
LOCUS       DASZMM010000374         5420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5375622, whole genome shotgun sequence.
ACCESSION   DASZMM010000374 DASZMM010000000
VERSION     DASZMM010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5375622"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VG754_09115"
     CDS             <1..537
                     /locus_tag="VG754_09115"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HWF19417.1"
                     /translation="DPLADFLGVSRISSADTSFTWSNRTNSMSIVTGGQLPIFADKDE
                     TLKALASPEFNPRATVYLPLAARRVVNITNQTHVQITMRNFSAQQIELDAQADAPAMV
                     VIAQAFYEPWHAYVDGRRVPLWQANAAFQALEVPAGKHQVTVMYEDTIFKAGAVISLG
                     TLLATGLSWIGLGRRRKS"
     gene            607..1479
                     /locus_tag="VG754_09120"
     CDS             607..1479
                     /locus_tag="VG754_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWF19418.1"
                     /translation="MSSHLEVLKTYKLYIGGNFVRSESGRYLPAKSPAGEHLDNYSHA
                     SRKDLRDAVVAARAAFDGWAKRTAYLRGQILYRAAEMLQNRTAELADEISRSTGVRSA
                     KARKEVMAAIDRLVYFAGWTDKYSQVFGSVNPVATSHFNFTTPEPTGVVVIFAPDEPA
                     LVALVSLIAPVILSGNTAVVIASEKHPLTALTFAEILATSDLPGGVVNLLTGKRAELA
                     PHAADHMDINAIVDGSGIAELGALLQAGSSKNLKRYSSNVHPPTDWFTSKAEDPYKIL
                     DTVEFKTAWHPIGI"
     gene            1609..2601
                     /locus_tag="VG754_09125"
     CDS             1609..2601
                     /locus_tag="VG754_09125"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWF19419.1"
                     /translation="MNTTQPLRVAVTGAAGQIGYSLLFRIASGAMFGPNQAVILHLIE
                     IEPALPALNGVVMELDDCAFPLLKGIVPTANLDEGFRGVNWALLVGSVPRKQGMERKD
                     LLGINGKIFIGQGQAIQKNAASDVRIHVVGNPCNTNCLIAMNNAPEVPKDRWFAMTRL
                     DENRAKSQLAKKAGAEVTAVTNMAIWGNHSSTQYPDFTHAKIGGKPATEVIKDEAWLK
                     GEFISSVQQRGAAVIKARGLSSAASAANAVVDSVASIANPTAPGDWHSVCVHSDGSYG
                     IETGLICSFPVRSNGQKLEIVQGLSVDAFSKSKIDATVAELKEEKSLVADLLPK"
     gene            2765..3418
                     /gene="deoC"
                     /locus_tag="VG754_09130"
     CDS             2765..3418
                     /gene="deoC"
                     /locus_tag="VG754_09130"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWF19420.1"
                     /translation="MDVVPTNLSSYIEAMFFASDATRADIEKFCAEAREKNYRAVCVN
                     GSRVELARELLEEANVQVAALVGFPLGTNDSDAKRYETEVAVDSGAQEIEFVINIGRL
                     KDGDRQFILREMRDIAEAADERSVKAILETHLLTRDEKILACQFALDSGIHFVVTSTD
                     FHAPAVTVDDVKLLRETLGEQLGVKAAGQIRDAQTAQALIEAGANRLGISAGQWKTG"
     gene            complement(3494..4564)
                     /locus_tag="VG754_09135"
     CDS             complement(3494..4564)
                     /locus_tag="VG754_09135"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HWF19421.1"
                     /translation="MDIIAATKSYEAWLSKLTPLIRQDIKLKHSQMREDVFCFLRATY
                     YRWAQIFPEACPALANDPQPLAVGDLHVENFGTWRDSDGRLVWGVNDFDECARLPFGH
                     DLVRLAVSAFLAIDDGDLGLTKGAAVTAILEGYNGSLKISGKPFVLVDRSSALRTMAR
                     ERLNNPERFWAKMVSHPPVRQTVPASALRAIRELLPERNLPLKFLHRIAGLGSLGKQR
                     FTGVGNWRGGPIVREAKALTVSACHWTHGGKGNGPIHYEEILRRAVRCPDPMVRVRGT
                     WLVRRLSPDCFRIRLSNLPQKRDERELLYSMGWETANIHLGTVKPNILLDNLKRKSRT
                     WLLENAVTMHDQTKKDWKAWRK"
     gene            4695..>5420
                     /locus_tag="VG754_09140"
     CDS             4695..>5420
                     /locus_tag="VG754_09140"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HWF19422.1"
                     /translation="MRLKYRHIFLVCWHGLLVLALTACGPSKDVSDKPAQIHGELIAP
                     SPGPQPKITAVAVAANPQPAPIPSLVAAVPVAPKPTTALDALASPQSAPPPEVRENVS
                     NAPEYAAVGFDKLASYNFEVDDRPLTNSTTGADKANNQIPDRVKALDQKKVSIRGFML
                     PLKVSNGVVTEFLIMKDQSMCCYGTVPKITEWVSVKTPEHGVKSIMDQPVIIEGTLHV
                     GAMRENGYLIGIYQMDGDKLIAPE"
BASE COUNT         1205 a         1518 c         1547 g         1150 t
ORIGIN      
        1 gacccgctgg ctgatttcct cggcgtgtcc aggatttcgt ccgcggatac ttcgttcaca
       61 tggtccaacc gcacgaattc catgtcgatt gtgacgggag gtcagttgcc aattttcgca
      121 gacaaagatg aaacattgaa agcgctggct tcgccggagt ttaatccccg cgcaacggtc
      181 tatcttccac tggcggcgcg cagagtcgtc aacatcacca atcaaacgca tgttcaaata
      241 acgatgcgca atttttccgc gcaacaaata gagttggatg cgcaagcgga cgcgcccgca
      301 atggtggtga tcgcgcaggc gttctacgaa ccatggcatg cttacgtgga tggcaggcgc
      361 gttccgttgt ggcaggccaa tgccgcgttt caggcgttgg aagtgcccgc cggaaaacat
      421 caagtcacgg tgatgtatga agacacaata tttaaagcgg gggcggtgat ttcgttggga
      481 acattgctgg caaccggctt gtcttggatt gggttgggca gacgacgtaa aagttgagca
      541 gcgttttgct ttcctcattg ccgcaagcgc gttttcctgt cacgattttg cggaatcaag
      601 caaccaatga gcagccatct cgaagttctc aagacctata agctctacat cggcggcaat
      661 tttgtccgca gcgaaagcgg acgttatctg cccgcgaaat cgcccgccgg cgagcatctc
      721 gacaattatt cccacgcctc gcgcaaggac ctgcgtgatg ccgtggtcgc cgcgcgcgcc
      781 gcattcgacg gttgggccaa gcgcaccgca tacctgcgcg ggcagatact ttatcgcgcc
      841 gcggaaatgc tgcaaaaccg cactgctgaa ctggcagacg aaatcagccg ttccacgggc
      901 gtgcgttcgg ccaaggcgcg aaaggaagtc atggcggcaa ttgatcggct cgtttatttc
      961 gccggctgga ccgacaaata ttcgcaagtg ttcggcagcg tgaatccggt ggcgacgtcg
     1021 cactttaatt tcaccacgcc ggagccgacc ggagtcgtgg tgattttcgc gccggatgaa
     1081 ccggctttgg ttgcgctggt ttcattgatc gcgccagtca tcctcagcgg gaacactgcc
     1141 gtggtgatcg cttcggagaa acatccgctc accgcgctga cgtttgctga aattctcgca
     1201 acgagcgatt tgcccggcgg tgttgtaaac cttctcaccg gcaaacgcgc tgaactcgcc
     1261 ccgcacgccg ccgaccacat ggacatcaac gcgatcgtgg acggctccgg catcgcggaa
     1321 ttgggcgcgc tcttgcaagc cggttcatcg aaaaatctga agcgttattc cagcaatgtt
     1381 caccccccca ccgattggtt caccagcaag gcggaagacc cgtataaaat tctggatacc
     1441 gtggaattca agactgcatg gcatccgatc gggatctaaa gttggttaca tggttacagc
     1501 gttacatagt tccgaatggc gtaaacgatg caaccatgta accacggcaa cgaacccact
     1561 tgcagtttcc ctaatcccgt tccataatgc gggactaatt taagacaaat gaatactaca
     1621 cagccccttc gtgtcgcggt caccggcgct gccggtcaga ttggatattc gctccttttt
     1681 cgcatcgctt ccggcgcgat gttcggcccg aaccaggcgg tcatcctgca tttgattgag
     1741 atcgagcccg ccctgcccgc gctcaacggc gtggtgatgg aactggacga ttgcgcgttt
     1801 ccgttgctca agggcatcgt tcccacggcg aatttggacg aaggttttcg cggggtgaat
     1861 tgggcgctgc tcgtcggcag cgtgccgcgc aaacagggga tggaacgcaa ggatttgctc
     1921 ggcatcaacg gcaagatttt catcggacag ggacaggcca tccagaaaaa cgccgccagc
     1981 gatgtgcgca tccatgttgt cggcaatcca tgcaatacga attgcctgat cgcgatgaac
     2041 aacgcgccgg aagttccgaa agaccgttgg ttcgcgatga ctcgccttga cgaaaatcgc
     2101 gcgaagtcgc aactcgccaa aaaagcgggc gcggaagtca ccgccgtgac gaacatggcc
     2161 atctggggca atcattcctc gacgcaatat cctgatttta cccacgcaaa aatcggcggc
     2221 aaaccggcaa cagaagtcat caaggacgaa gcatggctca agggcgaatt tatttcctcg
     2281 gtgcaacaac gcggcgcagc cgtcatcaag gcgcgcggtc tctccagcgc ggccagcgcg
     2341 gccaatgcgg ttgtggacag cgtggcttcg attgccaatc cgaccgcgcc cggcgactgg
     2401 cacagtgttt gtgtccactc ggacggcagc tatggcatcg aaacaggttt gatctgctct
     2461 ttccccgtcc gcagcaacgg ccagaaactt gaaatcgtcc agggtctttc cgtggatgca
     2521 ttcagcaagt cgaaaattga cgcaaccgtc gccgagttga aggaagaaaa atctttggtg
     2581 gcggacctgc tgccgaaatg agaacgtagc gcagagttgc gctctgctct atcgccgact
     2641 tgcagtcggc aagatgggaa aacggaaggc gcatacaagc ggaggcgctg tgctccagta
     2701 gtgcgcgcgg agtgaaactc cgcggtacag cagagtgcaa ctctgcgcta caatcggatt
     2761 aaatatggac gttgttccca caaaccttag ttcttacatc gaagcaatgt ttttcgcgtc
     2821 cgatgccacg cgcgctgata tcgaaaagtt ttgcgcggaa gcacgcgaga aaaattatcg
     2881 cgcggtttgc gtgaacggtt cgcgtgtgga actcgcccgc gagctgctgg aggaagcgaa
     2941 cgtccaagtc gcggcattgg ttggatttcc actcggcacg aacgattcgg acgccaagcg
     3001 ttacgagacg gaggtcgcgg tggattccgg cgcgcaggag attgaatttg tcatcaatat
     3061 tggccggctc aaggacggtg accggcaatt catcctgcgc gagatgcggg acatcgccga
     3121 agccgccgac gagcgatcgg tcaaagccat tctcgaaact catctgctca cgcgcgacga
     3181 aaagattctt gcgtgtcagt tcgcgcttga ttccggcatc cattttgtcg tgacttcaac
     3241 cgatttccac gcccctgcgg tcacggtgga tgatgtgaaa ttgctgcgcg aaactctcgg
     3301 agagcaattg ggcgtcaaag ccgccggtca aatccgcgac gcacagaccg cgcaagcatt
     3361 gattgaagcg ggcgcaaatc gcctggggat ttcggcagga cagtggaaga ctggttgaaa
     3421 aagggacgga aagccaggtt ttcaacattc agcgttcagc gttcgatgtt tgttgttcag
     3481 cgtttctccc atttcatttc cgccaggcct tccaatcctt cttcgtttgg tcgtgcatgg
     3541 tgacggcatt ttccagcagc catgtccgtg atttgcgttt gagattgtcg agaagaatgt
     3601 tcggcttgac ggtgccgaga tggatgttgg ccgtttccca gcccatggag taaagcagct
     3661 cgcgttcgtc gcgtttttgc gggaggttcg acagccggat gcgaaaacaa tcgggtgaca
     3721 accgccgcac cagccacgtt ccgcgcacgc ggaccatcgg gtcgggacag cgcacggcgc
     3781 ggcgcaaaat ttcctcataa tgaatcggac cgttgccctt gccgccatgt gtccagtgac
     3841 acgccgaaac cgtcagcgcc ttggcttcgc gcacaatcgg cccgccgcgc cagttgccga
     3901 cgcccgtgaa ccgctgcttg cccaggctgc caagcccggc gatgcggtgc aaaaatttca
     3961 acggcaaatt gcgttcgggc aatagttcgc gaatggcccg caacgcgctc gcaggaacgg
     4021 tttgtcgcac gggagggtga ctgaccatct tggcccaaaa tctttccgga ttgttcaaac
     4081 gttcgcgcgc catcgtccgc aacgcgctgc ttctgtccac caacacgaac ggtttgccgg
     4141 aaatttttag cgagccgttg taaccttcca aaatcgcggt gacagccgcg cctttcgtca
     4201 ggccaaggtc gccgtcgtca atcgccagga acgcgcttac cgccaatcgc accaagtcat
     4261 ggccgaacgg cagccgggcg cattcatcaa aatcgttgac gccccaaacg agccggccat
     4321 cgctgtcgcg ccaggtgccg aagttttcca catgcagatc gccgactgcg agcggttgcg
     4381 gatcgtttgc caatgcggga cacgcttcgg gaaaaatttg cgcccagcga taatacgtcg
     4441 cgcgcaggaa gcagaaaacg tcctcgcgca tttggctgtg cttgagcttg atgtcctgcc
     4501 ggatgagcgg cgtcagcttc gaaagccagg cttcgtaact cttcgttgcg gctatgatgt
     4561 ccatgcgcaa ggcatggttt aaagtttcag gttcgaagtt gaaagttcaa aatagggttg
     4621 aaactgcggt gtgccgctga tttgaatgtt tgaatccggg ggcgagtcag actattggtt
     4681 attgcgcgga catcatgcgg ctcaaatatc gccatatctt tctggtctgc tggcacgggt
     4741 tgctcgtgct ggcgctgacg gcctgcggac catcaaaaga tgtttcggat aagccggctc
     4801 agattcatgg ggagctgatt gcaccaagtc ctggcccgca accaaaaata accgcggtcg
     4861 ccgttgcggc caatccacag cctgcgccga ttcccagcct cgtggcagca gtgcctgttg
     4921 ctccaaaacc aacaacggct ttagatgctc tcgcatcgcc gcagtccgcg ccgccgccgg
     4981 aagttcggga aaatgtttcc aacgcgccgg aatacgcagc ggtcgggttc gataaactgg
     5041 cttcgtacaa tttcgaagtg gatgacaggc cgttgaccaa ctcgaccact ggcgcggaca
     5101 aggcgaataa tcagattccc gaccgcgtga aagcgttgga ccagaaaaaa gtttccatcc
     5161 gtggtttcat gctgccgctc aaggtgtcca acggcgtcgt gacggaattt ctgatcatga
     5221 aagaccaatc catgtgttgc tacggcactg tgccgaagat caccgaatgg gtcagcgtca
     5281 aaaccccgga gcacggcgtg aagtcaatca tggaccagcc agtcatcatt gaaggaacgt
     5341 tgcacgtcgg cgcgatgcgc gagaacggct acctcatcgg catctaccag atggacggcg
     5401 acaagttgat tgcgccggag
//
LOCUS       DASZMM010000375         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4822686, whole genome shotgun sequence.
ACCESSION   DASZMM010000375 DASZMM010000000
VERSION     DASZMM010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4822686"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2118
                     /locus_tag="VG754_09145"
     CDS             <1..2118
                     /locus_tag="VG754_09145"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HWF19423.1"
                     /translation="KEIATAAELRARAGDYLFAAYEAQQLTDKKDFDGIAAKYKLDGE
                     VIRRWMKGLDNWSKESNAIFTPWFAFAKLPEKDFAAKAKDLTAKWSTQSTATVSINPL
                     VAKAFTNEPPASMKEVAERYSKIFNEVDAHWHEALTSTNAPAPTKLPDMNQEALRRVL
                     YADNAPANLPVSEFNRLYDTPTSQKIRALQRSVDELDATHPGAPPRAMALVDRSEPVT
                     QHVFIRGNSGNEGPEVPRQFLEALAGPQRKPFHHGSGRLELAQAIVSTNNPLTARVFV
                     NRVWLYHFGVPLVATPSDFGLRSDPPTNPELLDYLASRFMADGWSVKKLHRLIMLSGT
                     YRQSSEENPRCAKADPGNQLLWRMNRQRLDFEAMRDTLLMVSGKLDLTAGGHSVDIID
                     AASTRRTVYGFIDRQNLPDLLRAFDFASPDTSSARRYYTTVPQQALFLMNSPFVIEQA
                     KGLVNRPDFKAARTEDEQVRLLYQLAFQRKPTAEEISLARQFTHGEVAKPEPPALTNW
                     VFGCGSFDAEAKRVKHFRKLPYFDGTSFQGGPSLPDPKLGYVMVTSSGGHPGDQQHDS
                     IRRWIAPRDGTIAITSTLEHNNAQGDGVHGRIVSSKRGVLGEWSVHNGKHEANVERVE
                     VKQGDTVDFVTDCNGNVDYDSFGWSPAIRFLADAKTLPGQRMEWDAQKDFAEAARSAH
                     PTMDAWQRYAQILLLTNELVFVD"
     gene            2137..>2697
                     /locus_tag="VG754_09150"
     CDS             2137..>2697
                     /locus_tag="VG754_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HWF19424.1"
                     /translation="MHPQLYIIAGPNGAGKTSFAKSYLPQYTNSREFVNADELAQKIS
                     PQNPEAAAIRAGREMLTRIKWLAAERADFSIETTLSGKSYVPWLRELKDGGYEIHLFF
                     LWLPDVQMCLDRVADRVRKGGHNIPATVIRRRYLAGIKNLFNVYRSLVDSWSLFDSSH
                     LVLYKIAKEKGGLLKVLDEERLAKFMK"
BASE COUNT          641 a          750 c          742 g          564 t
ORIGIN      
        1 aaggaaatcg cgaccgccgc tgaattgcga gcgcgagcgg gcgattatct gttcgctgct
       61 tacgaagcgc aacagttgac cgataagaag gattttgacg gcatcgcggc gaaatacaaa
      121 ctggatggtg aagtcatccg tcgttggatg aaagggcttg ataattggtc gaaagagtcg
      181 aatgcaattt tcacgccgtg gttcgccttc gcaaaattgc cggaaaaaga ttttgcggcg
      241 aaggcaaagg atttgaccgc caagtggtcc acgcaatcga ccgcgaccgt ttccataaat
      301 ccactggttg caaaagcatt cacaaacgaa ccgccagctt cgatgaaaga agtcgccgag
      361 cgttacagca aaatcttcaa cgaagtggac gcgcattggc acgaagcttt gacatccacg
      421 aatgcgccag cgccgacgaa attgccagac atgaaccagg aagcgcttcg gcgggtattg
      481 tatgcagata acgcgcctgc gaacctgccg gtcagcgaat ttaaccgcct ttacgacact
      541 cccacgagcc agaagatacg cgcattgcag cggagcgttg atgaactgga tgccacgcat
      601 cccggcgcgc caccgcgcgc gatggcgctg gtggatcgtt ctgagcccgt cacacaacat
      661 gtgttcatcc ggggaaacag cggcaatgaa ggtcccgaag tcccgcgaca atttttggaa
      721 gcgcttgccg ggccgcaacg caagccgttc caccatggca gcgggcgttt ggaacttgcg
      781 caagccatcg tcagcacgaa caacccgctc accgctcgag tgttcgtgaa tcgcgtttgg
      841 ctgtaccatt tcggcgtgcc actcgtggcg acgccgagcg attttggttt gcgttcggac
      901 ccgccgacca atcccgaact gcttgattat cttgcctcgc gcttcatggc agacggctgg
      961 tccgtcaaaa aattgcatcg gctcatcatg ctttccggca cttaccgcca aagcagcgag
     1021 gaaaatccgc gttgcgcgaa ggcggatccc ggcaatcaat tgctctggcg catgaaccgg
     1081 caacggcttg atttcgaggc gatgcgcgac actctcttga tggtttcggg aaaacttgat
     1141 ctgactgcgg gcggtcattc ggtggacatc attgatgccg cttccacgcg ccggaccgtt
     1201 tacggcttca ttgaccggca gaaccttccc gatttgttgc gcgccttcga ttttgcaagc
     1261 ccggacacca gcagcgcccg gcggtactac accaccgttc cgcaacaggc tttgttcctg
     1321 atgaacagcc cgttcgtcat cgagcaggca aagggcctcg tcaaccggcc ggatttcaag
     1381 gccgcgcgaa cggaagacga gcaggtgcgt ctgctttatc aactcgcgtt ccaacgcaaa
     1441 ccgaccgccg aagaaatttc tctcgcccgc caattcaccc atggcgaagt cgccaaaccc
     1501 gaaccgcccg ccctgacgaa ttgggtcttc ggttgcggca gttttgatgc ggaagcgaag
     1561 cgggtaaaac atttccgcaa acttccttat tttgacggca cctcgtttca aggcggaccg
     1621 tccctgcccg atccgaaatt gggatacgtc atggtcacca gcagcggcgg acatccggga
     1681 gaccagcaac acgattccat ccgccgctgg atcgcgccgc gcgatggcac gattgccatc
     1741 accagcactt tggaacacaa caatgcgcaa ggcgatggcg tgcatgggcg cattgtttcg
     1801 agcaagcgcg gcgtgttggg cgaatggagc gtgcataacg gcaagcacga agcgaacgtc
     1861 gagcgcgttg aagtgaaaca aggcgacacg gttgatttcg tcaccgattg caatggcaac
     1921 gtcgattacg attccttcgg ttggtcgccg gccattcgtt tccttgccga tgcgaaaacc
     1981 ctgcccggcc agcgaatgga atgggacgcg caaaaagatt tcgccgaagc cgcccgttcc
     2041 gcgcacccca ccatggacgc ctggcaaaga tacgcgcaaa tcctgctgct cacgaatgag
     2101 ttggtgtttg tggattgaac cgtcataatt cgcgcggtgc atccgcagct ttacatcatt
     2161 gccggaccga atggcgcggg caaaacgtcc ttcgccaaat cttatcttcc gcagtacacc
     2221 aacagtcgcg agtttgtgaa tgctgacgaa ctcgctcaaa aaatatctcc gcaaaatccc
     2281 gaagcagcgg caatccgggc gggacgcgaa atgctgacgc gaatcaaatg gcttgctgcg
     2341 gaacgagcgg atttcagcat cgaaacaaca ctttcgggaa aatcttacgt cccatggctg
     2401 cgggaattga aagatggcgg gtacgaaatt catttgttct ttctttggct gccagacgta
     2461 caaatgtgtc ttgaccgggt tgccgaccga gttcgaaaag gtgggcacaa cataccggcg
     2521 acagtgattc gccgtcgtta tctcgccggg ataaaaaacc tctttaatgt ttaccggtct
     2581 cttgtggatt cgtggagttt gtttgacagt tctcacctcg tgctttacaa gatagccaag
     2641 gaaaaaggcg gccttttgaa ggtattggat gaagaaaggc tagcaaaatt tatgaaa
//
LOCUS       DASZMM010000376         3830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5377347, whole genome shotgun sequence.
ACCESSION   DASZMM010000376 DASZMM010000000
VERSION     DASZMM010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5377347"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="VG754_09155"
     CDS             <1..1780
                     /locus_tag="VG754_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HWF19425.1"
                     /translation="QIREKVRFSEEAFHLLLAGGVGVIGGLVNLFYHNAIVLSQRFFM
                     HQTGEPVEIAAQLPYWERALIPTAGGLCAGLVLYFGLRLVGKQSSTNILEVVATSDGK
                     LPFRSGVVKALSSLVSIGSGASIGREGGITQLSATFASKWGQLARWHPYRLRLLTACG
                     AASGISAAYDAPITGAVFAALIVLGNFSMNLFGPLVFSSVVAAMVSRSFFGLKPLYVA
                     PSFDFSSLTELPWFLVLGILTGTMGSLFLKFLQASQDLFKKMPGSIYVRMLIGGVAVG
                     VIAIGFPGVWGNGYGTTNEILQRHFLVYSYPLLFLTGLLFAKLLATSAAVGSGAVGGV
                     ITPTLFLGAVTGAIEWTWLQSIGQGSELPTGAFALVGMGSMLAATTRSPLLAMIMIME
                     ISLNYSIMPPLMLACVISTLVARRLHPESIYTEPLRNKGLMVDREILRPGSATEQKVG
                     DLMRAPVPPLRDTASLQEIAERFLISPNNFLPVVDAKYQLIGVVALHDLKEYLNAGSE
                     IMGVIAADVMRPPPAAVTPDQLLLDTLPIMLASEQRNVPVVNSHSENRLVGAVVRAEV
                     LGLLSEAIATKAEPIKPPEELTGAQH"
     gene            complement(1760..2755)
                     /locus_tag="VG754_09160"
     CDS             complement(1760..2755)
                     /locus_tag="VG754_09160"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWF19426.1"
                     /translation="MSFPFNVYLPAFAGAFIVSLAALPCWRVICRRIGLVDDPGHRKI
                     HETPMPLAGGLAVLTGMVLPLLLGVAMVQWNIPRLPGAHGLLEHGLDVRRTELIAIVL
                     GAVGIVVTGWLDDQYELKPLVKFTGQLLVAALIAVSGIRITLFVHSTLFSYAVTILWM
                     LTVINAFNFMDNMNGLCAGLGAIGSWFFALIAIMDGQYLVALLALLACGALLGFLPYN
                     FPKASAFLGDTGSHLVGYLLAVMAILPHFYTAQHPRRWSVLIPLLILAVPLGDLAYVV
                     MLRWKLGKPFYHGDTNHLSHRLVKRGLTKQKTVLLIWLAAAMLGGAAAALMLRPG"
     gene            complement(2752..>3830)
                     /locus_tag="VG754_09165"
     CDS             complement(2752..>3830)
                     /locus_tag="VG754_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF19427.1"
                     /translation="EAHLISGNDTGPEGSLANAFAHSPQTLTLIPELVRPVHPWKDLL
                     AYRQLTGIFRTQKPDIIHTHSGKAGVLGRLAAHKARVPVIVHTIHGPSFGPFQGGAAN
                     FVFRSAERRAGKVTTHFVVVADAMTRQYLEAGIASPLQYTKILSGFPLQPYLSATNDI
                     AVRARFGIAPDDIVIGKNARLFKLKGHDDLFSIAPELVRRVPRIKFLLVGDGPWRQRF
                     EQQARSLGLEKQFIFTGLVPPEEVPRLVGIMDILVHLSRREGLARALPQALAAARPII
                     AYDCDGANEVCLDNETGFLVRVGDLVGLSERLVKLAGDAALRERFGRQGREFVRERFA
                     VETMVRDLFSLYLKLAPGIRMPPS"
BASE COUNT          822 a         1101 c         1123 g          784 t
ORIGIN      
        1 gcagatccgc gaaaaagtgc ggttcagcga ggaagcgttc catctgctgc tggctggcgg
       61 tgtgggtgtc atcggcggcc tcgtaaatct tttttaccac aatgccatcg tgctatcaca
      121 acgcttcttc atgcaccaaa caggcgagcc ggtggaaatc gccgcccaac ttccctattg
      181 ggagcgggcg ttgattccga cagcaggcgg gctttgcgcc gggcttgtgc tttacttcgg
      241 cttgcggctc gtcggcaaac aaagctcgac gaacatcctt gaagtcgtgg ccaccagcga
      301 tggcaagctt ccgtttcgtt ccggggtcgt gaaggcgctt tcatcgctgg tgagcatcgg
      361 cagcggcgct tccatcgggc gcgaaggcgg catcacgcag ctttccgcga cgttcgcctc
      421 caaatggggt cagttggcgc gttggcatcc atatcggttg cgattgctga cggcgtgcgg
      481 cgcagcgtcg ggaatttcag cggcatacga tgcgccgatc accggggcgg tttttgcggc
      541 gctgattgtg ctcggaaatt tctcgatgaa cttgttcggg ccgctggtgt tttcctccgt
      601 ggtggcggcg atggtatcga gaagtttttt cggcctaaaa ccgttgtatg tcgcgccttc
      661 gttcgatttc tcgagcctga cggaactgcc gtggtttctt gtccttggaa tcctcaccgg
      721 aacgatgggt tcgctttttc ttaagttcct tcaggcgagc caggacttgt tcaagaaaat
      781 gccgggcagc atttatgtaa ggatgttgat tggcggggtg gcggtgggag tcatcgcgat
      841 cggctttccg ggagtgtggg gcaacggcta cggcacgacg aacgaaattt tgcagcgcca
      901 tttcctcgtt tattcctacc cgcttttgtt cctgaccgga ttgttgttcg caaaactgct
      961 cgcgacatcc gcggcggttg gttccggcgc ggtgggcggc gtgataacac cgacgctgtt
     1021 tcttggcgcc gttaccggcg caatcgagtg gacatggctg cagagcatcg ggcaggggag
     1081 cgaattgcca acgggcgcat ttgcgctggt tggcatgggc agcatgttgg ccgcgacgac
     1141 gcgttcgccg ttgcttgcca tgatcatgat catggaaatc tcgctgaatt attccatcat
     1201 gccacctctg atgcttgcgt gtgtgatctc cactctcgtt gcgcggcgtc tgcatcccga
     1261 atccatttac accgaaccgc tgcgcaataa aggcctgatg gtggaccgtg aaattttgcg
     1321 tcccggttcc gcaacggaac aaaaggttgg cgacctgatg cgcgcgccgg tgccgccatt
     1381 gcgcgacacg gcgagcttgc aggagattgc cgaacgcttt cttatcagcc cgaacaattt
     1441 tctgcccgtc gtggacgcaa aatatcaact catcggggtg gtcgcgttgc acgacttgaa
     1501 ggaatatctc aatgccggtt ctgaaatcat gggcgtgatc gccgccgatg tgatgcgtcc
     1561 gcccccggct gccgtgacac cggaccagtt gctgctcgac acgctgccca tcatgcttgc
     1621 cagcgagcag cgcaacgtgc cggtcgtgaa ttcacattcc gagaaccggc tggtcggcgc
     1681 ggtcgttcgc gcggaagtgc tgggtctgct ctccgaagcc atcgcgacca aagccgagcc
     1741 gatcaaacct cccgaggaat taaccggggc gcagcattag cgcggcggcg gctccgccca
     1801 gcatcgccgc ggcaagccag atgagcagca cggtcttttg tttcgtcaaa ccgcgtttta
     1861 ccaaacggtg tgaaaggtgg ttcgtgtccc cgtgataaaa tggttttccc aacttccagc
     1921 gcaacatgac cacatacgcg agatctccga ggggaacggc aaggatgagc agcggaatga
     1981 gcaccgacca gcgccgcgga tgttgcgcag tgtaaaaatg cggcagaatg gccatcaccg
     2041 ccagcaggta accgaccaga tggctgccgg tgtcgcccag gaatgcgctg gctttcggga
     2101 aattataagg cagaaaaccg agcaatgcgc cgcaggccag caacgcgagc agtgcgacca
     2161 aatattgccc atccatgatc gcgatcagcg caaaaaacca ggaaccgatc gcgcccaacc
     2221 ccgcgcaaag gccgttcatg ttatccatga aattgaaggc attgatgacc gtgagcatcc
     2281 acaagatagt gacggcgtag ctgaaaagcg tgctgtgaac gaagagcgtg atgcggattc
     2341 ccgacaccgc aatgagcgcg gcgacaagca attgacccgt gaatttcacc aacggcttca
     2401 attcgtattg gtcatcgagc cagccggtga cgacgatgcc aaccgcgccg agcacgatgg
     2461 caatcaactc agtcctgcgg acatccaatc catgttccaa caaaccgtgg gcgccgggca
     2521 gtcggggaat gttccattgc accatcgcca cgccgagcag aagaggcagc accatgccgg
     2581 tcagcaccgc aagacctcct gccagaggca tgggtgtttc gtggattttc cgatggcccg
     2641 ggtcatccac caggccgatg cggcggcaga tgacccgcca gcagggaagc gcagccaacg
     2701 aaacaataaa cgccccggcg aaagccggca gataaacatt gaacggaaaa ctcacgacgg
     2761 cggcattcta attccgggag ccagcttcaa gtaaaggctg aataaatccc gcaccatcgt
     2821 ttccaccgcg aagcgctccc ggacaaactc gcgcccttgt cttccaaacc gttcgcgcaa
     2881 ggcggcatcg ccggccaatt tgaccagccg ttcgcttaac ccgacaaggt cgccgacgcg
     2941 tacgagaaag cccgtttcat tgtccaaaca gacttcattc gcgccgtcgc aatcataggc
     3001 aataatcggg cgcgcggcag ccagcgcttg cggcaatgcg cgggcaagcc cttcgcggcg
     3061 cgacaagtgg accagaatat ccatgatgcc caccagtcgc ggaacttctt caggcggcac
     3121 cagtccagtg aaaataaatt gcttctccaa tccaagcgaa cgtgcctgtt gctcgaagcg
     3181 ctgacgccag ggaccatcac ccacaagcaa aaattttatg cgcggaacgc ggcgcaccag
     3241 ttccggcgcg atgctgaaaa ggtcatcgtg ccctttcagt ttgaaaagcc gcgcattctt
     3301 gccgatgaca atgtcatcag gcgcgatgcc gaatcttgcg cgcactgcga tgtcgttcgt
     3361 ggccgagaga tacggttgca gcggaaagcc gctgaggatt ttcgtgtatt gaaggggcga
     3421 agcgatgcca gcttcgagat attgccgcgt catcgcatcg gcaacaacga cgaaatgagt
     3481 ggtcactttc cccgcgcgac gttccgcgga gcgaaacaca aaatttgccg cgcctccttg
     3541 aaatgggccg aatgatgggc catggatcgt atggacaatc accggcactc gcgctttgtg
     3601 cgcggccagt cgccccaaca caccggcctt gccgctgtgc gtatggataa tgtccggttt
     3661 ttgcgtccgg aaaatccccg tcaactgccg ataagccagc aaatctttcc acggatggac
     3721 cggacgcacc aattcgggaa tgagcgtgag cgtttgcggg gaatgagcaa aggcattggc
     3781 gagcgaacct tccggcccgg tgtcgttgcc ggaaatcaaa tgggcttcaa
//
LOCUS       DASZMM010000377         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4822730, whole genome shotgun sequence.
ACCESSION   DASZMM010000377 DASZMM010000000
VERSION     DASZMM010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4822730"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VG754_09170"
     CDS             <1..901
                     /locus_tag="VG754_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19428.1"
                     /translation="DDSQGDARYAEAQEIRSDAKYNLPAGAHMLYAGDWNLFNGSGEN
                     AYKCLTGQTTPDGINWADTNSIWANTNSTQGYDPTSKTSPPTTTIFANSSKDSVTYLY
                     DDSTDAGSFAMSSRLDVQLPNALMFAAYNSKGGVQLASDTNDPYDSSNFPSSQYQYAF
                     EVFGNNGTTPRGSTVTYSGNHALDDVTNAATVKLDMMRNTTNSLGSDHYPIVGDYNIV
                     VPASAPARPVLTPQGFGTNGSFQFKVASTTNTGFEIQASTNLLNWTNIASGYTGTNGL
                     LLFQDTNAAGFSKRFYRVLWPIP"
     gene            complement(927..1625)
                     /locus_tag="VG754_09175"
     CDS             complement(927..1625)
                     /locus_tag="VG754_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HWF19429.1"
                     /translation="MSFPANNSWFLLRSGAGTAAINMAMDEALLENAPSLGQPVLRFY
                     SWSEPAASFGYFQKYAEIEKTTLLRPLVRRPTGGGLVPHDVDWTYSIAVPPSAEWYAL
                     KAVESYKRVHEWIRAAFLKMGVNTELADCCRKALPGQCFAGHEKFDVLWQGRKIAGAA
                     QRRTKNGLLIQGSVQPPPTKISRSDWERAMCDVAQAEQGILWENFVPTPALNERVTEL
                     AAKTYSTAEYNQRR"
     gene            complement(1740..2654)
                     /locus_tag="VG754_09180"
     CDS             complement(1740..2654)
                     /locus_tag="VG754_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWF19430.1"
                     /translation="MYSFSTCWNSHRHTDGRAMLREIRELGFDHAELSHGTRISLLPG
                     AIDAVEAGEIKISSLHNFCPLPIGVNQSAPNLYRLSAEKQVERENAYRYTRKTIEMAA
                     RLKSPVVVLHYGSMDMKDYTDKLLDMVARGEKETPKYEKLCEEIMRKREAIKEPYVDR
                     ANELLKRLLPLAEENGIKLGIENREALEELPLEPDYNLLFRQLTSPAAVYWHDTGHAQ
                     IKENLGFIHHAMHLESQRERLAGFHIHDVQFPGRDHCAPGAGTIDFAALKPMVKPEHI
                     KVFEFSPALTVDEVKSGVEHVKKIWGKE"
     gene            complement(2720..2917)
                     /gene="rpsU"
                     /locus_tag="VG754_09185"
     CDS             complement(2720..2917)
                     /gene="rpsU"
                     /locus_tag="VG754_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWF19431.1"
                     /translation="MTEIKLKKGEPVEKALRRLKKKIDREGTLKVVRNHRHFEKPSER
                     RRRKMKAARFSAMLSARYADL"
     gene            complement(3080..4786)
                     /locus_tag="VG754_09190"
     CDS             complement(3080..4786)
                     /locus_tag="VG754_09190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWF19432.1"
                     /translation="MEESSDSRFGCVQRLIRLVKARPQLTLVLLTLAALGPFIAKPFN
                     IDDPLFLWAAKHIQANPANPYGFNVNWYGTPMPMWEVTKNPPLACYYLAASASILGWS
                     EMALHFAFLLPALLIVLGTYRLARQFCDQPLFAAVTTLFTPVFLVSSTSIMCDVLMLT
                     FFVWAVLFWVEGIAKRDHWRLIASAFLVTFAVYTKYFGACLIPLLAVYGLMKERRVGF
                     WLISLLIPIAGLVAYQWITFRLYGKGLLTDAGDYATDTKFLRISELPASTMTALAFTG
                     GCSAVATLLTPWLWRRRTAGLITLGMLILTLAGFAGSFWKRYYFLNSVSHGWLELEVV
                     FWSIGGVSVLGLALVDLWHRRDAESCLLALWVLGTFEFAGFLNWTVNGRSILPMAPAV
                     AILLARRLSPILHADQKKFAPIAICSIAAAALALLVTLADYDFAQTCRRSARETYDKY
                     GHGTAGLWFTGHWGWQYYMEQLGVKTANMTHPPPSNGLFLALPTHNVNLMPLNEKIVH
                     KHDVFAVNDGRLVSTMSGDIGAGFYSSIWGPVPFAFGNVPPETVRIYVIDVPQAPATH
                     QP"
     gene            complement(4869..>5680)
                     /locus_tag="VG754_09195"
     CDS             complement(4869..>5680)
                     /locus_tag="VG754_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWF19433.1"
                     /translation="LGIIERPGSADTILDALRRGLQLLEEKKIRAELVTITGEPIREI
                     IKRTEEHHFDLVVIGATRKGSRGLFWISSKAYKIIKTIHPPVLVVLEKSSRIKRILLC
                     TGGQKYIANAVTLTGQIARGMQASVTLFHVMPEPPPIYARLYRLQTDVDAVLNSKSEL
                     GRNLREQKKILEDLGVNVEVKLREGSVISEILREIQAGNYDLLVAGSSLSRASLRTYI
                     LGDITREIVNRACCAVLIVRSGITGEGILHNLSGWLERITHHHKPEKTGRQ"
BASE COUNT         1343 a         1561 c         1519 g         1257 t
ORIGIN      
        1 cgatgattcg cagggagatg cgcgttacgc ggaggcgcag gaaatccgca gtgacgccaa
       61 atataatctg ccggcgggcg cacatatgct ttacgcgggc gactggaatt tgttcaacgg
      121 cagcggtgaa aatgcctaca aatgcctgac aggccagacc actcccgacg gaatcaattg
      181 ggcggacacc aattccatct gggccaatac caattcgacg caggggtacg accccacatc
      241 caagacaagc ccgccaacaa ccacaatatt cgcaaacagc tccaaggata gcgtcacgta
      301 tctttacgat gattcgaccg acgcgggcag tttcgcgatg tcatcgcgcc tcgatgtcca
      361 attgccgaat gctttgatgt ttgcggctta caacagcaag ggcggcgtgc aattggcatc
      421 tgatacgaac gatccttacg attcgtccaa tttcccgtcc tcgcaatatc aatatgcttt
      481 cgaggtcttc ggcaacaacg ggaccactcc tcgcggaagc accgtgacct attcgggcaa
      541 tcatgcgctg gatgacgtga ccaatgccgc caccgtgaaa ctcgacatga tgcgcaacac
      601 cacaaattcc ctcggcagcg atcattatcc catcgtcggg gattacaaca tcgttgtccc
      661 ggcatcggcg ccagccaggc cagtcttgac tccgcaaggt ttcggaacga acggaagttt
      721 tcagttcaaa gtggcttcga cgaccaacac gggattcgag atacaggctt caactaatct
      781 gcttaactgg accaacatcg cctccggtta caccggcacg aacggattgc ttttgtttca
      841 agacacgaat gcggctggtt tctcgaagcg cttctaccgc gtgctttggc cgataccgtg
      901 attcggagcg atgtagtgag ttccattcat cgccgctggt tatattccgc agttgaataa
      961 gtttttgcgg ccagttcagt tactcgttca ttcagtgcgg gggttggaac gaaattctcc
     1021 cacaaaatcc cttgttctgc ttgcgccacg tcgcacatgg cccgttccca atctgagcgt
     1081 gaaattttgg ttggcggcgg ttgcaccgag ccttggatga gcaagccgtt ttttgtgcga
     1141 cgttgcgccg cgccggcaat cttgcgtccc tgccagagca catcaaattt ttcatggccg
     1201 gcgaagcatt gccccggcag cgctttccgg cagcaatcgg cgagttctgt gttcacgccc
     1261 attttcaaga acgccgcgcg tatccattca tggacgcgct tataactttc caccgccttg
     1321 agcgcatacc attccgcact cggtggaact gcgatgctgt aagtccagtc aacgtcatgc
     1381 ggcaccaatc ccccaccagt cggccggcga acaagaggac gcaacagcgt cgtcttttct
     1441 atctccgcat atttctgaaa ataaccgaaa gaagccgccg gttccgacca actgtaaaag
     1501 cgcaacacgg gttggccgag cgacggcgcg ttttccagca acgcttcatc cattgccatg
     1561 ttgatggcgg cggtgcctgc gccagagcga aggagaaacc atgaattgtt tgcagggaat
     1621 gacacgtgag cgaggaaaaa tgttaaatgg ttacattgtt acatggctag atcgttacaa
     1681 tgttgcagat cggccaaatt ttgtaaccat gtaaccattt aacattgtaa ccaatcaacc
     1741 tattccttcc cccaaatctt cttcacgtgc tcaacgccgc ttttgacttc gtccacggtc
     1801 agcgcgggac taaattcgaa caccttgatg tgctcgggct tgaccatcgg tttgagcgcg
     1861 gcgaaatcaa tcgtgccggc gccgggggca caatggtcgc gtccgggaaa ttgcacgtca
     1921 tgaatatgaa acccggcgag ccgttcgcgt tgcgattcca aatgcatcgc gtgatgaatg
     1981 aagccgaggt tttccttgat ttgcgcgtga ccggtgtcgt gccagtaaac tgccgcggga
     2041 ctggtgagtt gacggaacaa gagattgtaa tccggctcaa gcggaagttc ctccaatgcc
     2101 tcgcggtttt cgatgcctaa tttgatgcca ttttcttccg ccaacggcaa caggcgcttc
     2161 aacaattcgt tcgcgcgatc aacgtaaggc tccttgatgg cctcgcgttt gcgcatgatt
     2221 tcttcgcaaa gcttttcgta tttgggcgtt tctttttcgc cgcgcgccac catgtcgagc
     2281 agtttgtcgg tgtaatcttt catgtccatg ctgccgtaat gcagcacgac cacgggggac
     2341 ttgagccgcg cggccatctc gatggtcttg cgcgtgtaac gatacgcatt ctcgcgctcc
     2401 acctgttttt cggcggaaag ccggtaaagg ttcggcgcgg attgattcac gccgatgggc
     2461 agcggacaaa aattgtgcag gctggaaatc ttgatttcgc cggcttcgac ggcgtcaatc
     2521 gcgccgggca gcaggctgat gcgcgtgccg tggctcagtt cggcgtggtc gaagcccagc
     2581 tcgcggattt cgcgcagcat cgcgcgacca tcggtgtgcc ggtgggaatt ccagcaggtc
     2641 gaaaacgaat acataaagca aaggccgaat gcggacaatc tttcgaccgc attcggccat
     2701 gagaattcaa ttcacggaat cacaaatccg cgtagcgggc gctcaacatg gcggagaaac
     2761 gggccgcttt catcttgcga cggcgccgtt cgctcggctt ttcaaagtgc cggtggttgc
     2821 gcacgacctt caacgtgcct tcgcggtcga tttttttctt caatcgacgc agggcctttt
     2881 cgactggctc gccttttttc agtttaattt cggtcaactc aattcacatc ctctccgggg
     2941 accttggcaa cggcagcgcg gggccagccg atactcttca gtcaagccca agaggttatt
     3001 ttcggacgcg ccacatgtca accggcaaaa aacacccatt tttcacgtgc gaaagcgcgg
     3061 aaaacatgga ttggaagcgc taaggttggt gcgtcgccgg ggcttgcggc acatcaatga
     3121 cgtagattct aacggtttcc ggcgggacat tgccgaatgc aaaagggaca ggcccccaaa
     3181 tggaggaata aaagcccgcg cctatatctc cactcattgt ggagaccaac cgcccgtcgt
     3241 tgacagcaaa cacgtcatgc ttgtgcacga ttttttcgtt aagcggcatc aaattcacgt
     3301 tatgggttgg caacgcaaga aaaagaccat tggagggggg ggggtgcgtc atgttcgccg
     3361 tcttaacgcc gagttgttcc atgtaatatt gccatcccca atggcccgtg aaccacagcc
     3421 cggcggtgcc atggccatac ttgtcataag tttctcgcgc gcttcggcgg caggtttggg
     3481 cgaaatcata gtccgcaagg gtgaccaata acgccaatgc ggcagccgcg attgaacaaa
     3541 ttgcgatggg cgcaaacttt ttttggtcgg catgaaggat tggagacagc cggcgagcca
     3601 gtaaaattgc cactgccggc gccatgggca ggatggagcg tccattcacg gtccagttta
     3661 aaaaaccggc gaattcaaaa gtgcccagta cccacaaggc aagcagacag gattcggcat
     3721 cgcgccgatg ccaaaggtcc accaaagcca acccaagcac actcacgcca ccaatgctcc
     3781 agaaaacaac ttccaattcg agccagccgt gggaaactga atttagaaag taataccttt
     3841 tccaaaatga cccggcaaaa cctgccaatg tcaaaatgag catgcccaaa gttatcaacc
     3901 ccgcggtccg cctgcgccat aaccacggtg tcagcagggt tgccactgcg gaacatccgc
     3961 cggtgaaagc cagcgcggtc atggtgctgg cggggagttc tgagattcga aggaattttg
     4021 tatccgtcgc ataatctccc gcgtcggtga gcagcccctt gccgtacaaa cgaaaagtga
     4081 tccattgata cgcaaccaaa ccagcgatcg ggatgagcaa agaaatcaac cagaagccca
     4141 cccggcgttc tttcatcagg ccataaacag ccaggagcgg gatcaagcag gcaccaaaat
     4201 atttggtgta aacggcaaac gtcaccagga aggcggaagc gatcaaccgc caatgatccc
     4261 gcttcgcgat tccttccacc caaaatagaa ccgcccacac aaaaaacgtg agcatcaaga
     4321 catcgcacat gattgacgtg cttgacacca gaaaaaccgg tgtgaacaag gtggtgacag
     4381 cggcgaacaa cggctggtca cagaattggc gtgccaaccg gtacgtcccg agcacgatca
     4441 acaatgcggg caggagaaaa gcgaagtgca aggccatttc gctccagccc agaatggatg
     4501 ccgacgcggc cagatagtaa caagccaaag gcggattttt cgtgacctcc cacatcggca
     4561 tcggagttcc ataccaattc acattgaaac cgtacgggtt ggctggattg gcttggatat
     4621 gcttggctgc ccatagaaaa agcgggtcat caatgttgaa tggcttggca atgaacggcc
     4681 caagtgcagc caatgtgagc aacaccagtg tcaactgcgg ccgcgctttg accaggcgaa
     4741 tcaaccgctg gacgcacccg aaacgggaat ctgagctttc ttccactgct cattcttata
     4801 gtggctttga taatttcttg aaaacacttt ttgcattcac ctgaaaaatc gaaatgtccg
     4861 gccgagtgct attgtcttcc ggttttttcg ggtttgtgat gatgcgtgat gcgttccagc
     4921 cagccgctga ggttatgcag gatgccttcg ccggtgattc cggaacgcac gatcagcacc
     4981 gcgcaacagg cgcggttgac gatttcgcgc gtgatgtcgc ccaaaatgta agtgcgcagg
     5041 cttgcccggc tcagcgaaga accggcgacc aacagatcat aattgccggc ctggatttcg
     5101 cgcagaatct cggagatcac ggaaccttcg cgaagtttga cttcgacgtt cacgccgagg
     5161 tcttcaagaa tttttttttg ttcgcggaga ttgcggccca attcggactt ggaattgaga
     5221 acggcatcca catcggtttg gaggcgataa agccgggcat aaattggcgg cggttccggc
     5281 atgacgtgga acagcgtgac ggaagcctgc atcccgcgtg cgatctggcc ggtcagcgtg
     5341 accgcattgg cgatgtattt ctgaccgccg gtgcagagca ggatgcgctt gatgcgagag
     5401 cttttttcca gcacgacaag gaccggcgga tggatggtct tgatgatttt gtaggccttc
     5461 gaagaaatcc aaaaaagtcc ccgcgaacct ttgcgcgtcg cgccaataac caccaggtcg
     5521 aaatgatgct cctccgtccg tttgatgatt tcgcggatgg gctcaccggt gatggtgacc
     5581 agttcagcgc ggattttttt ttcctcgagc aattgcaacc cgcgccgcag ggcatcaagg
     5641 atcgtatcgg cgctgccggg ccgttcgatg attccgagca
//
LOCUS       DASZMM010000378         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5377450, whole genome shotgun sequence.
ACCESSION   DASZMM010000378 DASZMM010000000
VERSION     DASZMM010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5377450"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VG754_09200"
     CDS             <1..702
                     /locus_tag="VG754_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF19434.1"
                     /translation="SVLALDKLTGKEVWRALNDPVSNSSPIMITANGKRQLIVWSDSS
                     LASLNPSDGRTYWREPMTTSINVSAATPVFQENRLLVSGLMIDVSADAPRFLWPENRA
                     PSKRVLSNTSTGLLQGEYIYTARGLGELVCLEAATGRQIWSTNSVTGTKNGASINITP
                     QGDGVFLFTDEGNLIRAQLSPRGYREISRSHLIDPTWPFGQTKFVYAPPAFANRHVFA
                     RSEAEVVCASLEATR"
     gene            818..1750
                     /locus_tag="VG754_09205"
     CDS             818..1750
                     /locus_tag="VG754_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWF19435.1"
                     /translation="MNNPFTRRDFLKTSSAMLAATGFGGASLMAKDSSASAPAKKRDL
                     KRGIMFGTVPGNLPVMDKFKMIRDAGFDGVEANGSMNNDEILKARDTAGLMIPSVCDA
                     VHWAKTLSDPNPGAREIGLEGLKTALRDCKAYGGSSVLLVPAVVNQNVSYADAYERSQ
                     AEIRKAIPLAEKLGVKIAIENVWNQFLLSPLEAARYVDEFDSPAVGWHFDVGNVITYG
                     WPEQWIRILGKRIQKLHIKEYSRKKRDDEGLWKGFKVDYLEGDNNWPVVMQALDEIGY
                     KGWAIAEPAYHPPGLDDFGFLKHVREKMDEILAL"
BASE COUNT          463 a          482 c          569 g          415 t
ORIGIN      
        1 agtgtgctcg ctttggataa attgacgggg aaggaggtct ggagagcgct caatgatcct
       61 gtgtccaata gttctccgat aatgatcacc gccaatggga agcggcagtt gattgtttgg
      121 agcgacagtt cgctcgcgtc actcaaccct tccgacggcc gcacttattg gcgcgaaccg
      181 atgaccacga gcatcaatgt ctctgccgcc acgccggttt ttcaggagaa ccggctgttg
      241 gtgagcggac tgatgattga cgtgagcgcg gacgctccgc gctttctttg gcccgagaat
      301 cgcgcccctt caaaacgggt tttgagcaac acctcaaccg gactgctgca aggggaatat
      361 atttacaccg ccagaggctt gggcgaactc gtttgcctcg aagcggcgac cggcaggcaa
      421 atctggagca cgaacagcgt gaccggcacg aaaaatggtg cgagcatcaa cataactccg
      481 caaggcgacg gggtttttct atttacagat gagggaaacc tgatccgcgc tcagttgtcc
      541 ccaaggggct atcgagaaat aagccgcagc cacttgattg atccgacgtg gcccttcggg
      601 caaactaaat ttgtctatgc cccgccggct tttgccaacc gccatgtatt tgcccgcagt
      661 gaggcggagg tggtttgtgc ctcgctggaa gccacgcgat aaaggaagtg aaaagcgggt
      721 gaatgagtgg taaacgtggc gccgagcctg ttaataatga aacccaatgc ggaattctaa
      781 agtggaacta gagccggaaa cgctgtttaa tattcgcatg aacaatccat tcacccggcg
      841 cgattttttg aaaacgagca gcgcgatgct tgcggcgacc ggattcggcg gcgcttcgtt
      901 gatggcgaaa gattcatccg catccgctcc cgccaaaaaa cgcgacctca agaggggaat
      961 catgttcggc actgtgccgg gcaatttgcc cgtgatggat aagttcaaga tgatccgcga
     1021 cgcagggttc gatggcgtgg aggccaacgg cagcatgaac aatgacgaaa tcctcaaggc
     1081 gcgcgatacc gccgggttga tgatcccgag cgtgtgcgat gcggtgcatt gggccaagac
     1141 cctttccgat ccgaatccgg gcgcgcgcga aatcggattg gaagggctca agaccgcgtt
     1201 gcgcgattgc aaggcttatg gcggttcctc cgtgttgctt gtgccagcgg tggtgaacca
     1261 aaatgtttct tacgcggacg cctatgagcg ttcgcaggcg gagattcgca aggcgattcc
     1321 gctcgcggaa aaattgggcg tgaagattgc catcgaaaat gtttggaacc aatttctgct
     1381 cagcccgctt gaagccgcgc gttacgtgga tgaattcgac agcccggccg tcggctggca
     1441 ttttgatgtc ggcaacgtga tcacatacgg ctggcccgag cagtggatcc gcattctcgg
     1501 caagcggatc caaaaattgc acatcaagga atacagccgc aaaaagcgcg acgacgaagg
     1561 tttgtggaaa ggtttcaagg ttgattattt ggagggcgac aacaactggc cggtggtgat
     1621 gcaagcgctg gatgagatcg gctacaaggg ttgggcgatc gccgagccgg cgtatcatcc
     1681 gcctggtctc gatgatttcg ggtttctcaa gcacgtccgt gaaaagatgg atgagatttt
     1741 ggcgctttga tgaagccaga gttttgaacc accaagacac aaagacataa agacaccaag
     1801 gaaagctatt ccttctgtcg caaatgcggc tggagtaatt tgtccaactc gtcaggaagg
     1861 cgccgtaatt tttcgtccaa gccggtgcaa gcatgaaaag tttcagcttt caaaacgagg
     1921 atgtcgctt
//
LOCUS       DASZMM010000379         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3176473, whole genome shotgun sequence.
ACCESSION   DASZMM010000379 DASZMM010000000
VERSION     DASZMM010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176473"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VG754_09210"
     CDS             complement(<1..792)
                     /locus_tag="VG754_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWF19436.1"
                     /translation="MPGKTLVQYSGPTWNNEELEAALFAILCGKWLSAGENVAQFERQ
                     FSKHLGRKYSVMCNSGSSANLLMMSTIKSRRSYGFKDIEIITPVVCFPTTVAPIIQCG
                     FVPKFVDIDPASLNLDLDQVEKAIGPSTKAISFAHVLGNPPDMDRVMSICKERNLLLL
                     EDNCDALGSTWRGKPLGSFGLMSSCSFYPAHHITTGEGGMVSTDDETLDQILRSIMSW
                     GRDCYCIGAANLSLSGTCGKRFSDWLAPGYAGILDHKYIYQEVGYN"
     gene            complement(902..1252)
                     /locus_tag="VG754_09215"
     CDS             complement(902..1252)
                     /locus_tag="VG754_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19437.1"
                     /translation="MTNLIPLPITQTYHTMFLADDPQFSQRHNLMACALKDGIKAATR
                     LYECSRNAARKKGSRGYKSPADILATKAPQISPRIFLLHPVPLESLLPKRVTMYRGAP
                     IDRVYTLYLVKGER"
     gene            complement(1301..1531)
                     /locus_tag="VG754_09220"
     CDS             complement(1301..1531)
                     /locus_tag="VG754_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWF19438.1"
                     /translation="MPIYEFHCEKCEKDSEILVRSSEWEGTKCPHCGSTKLSKKLSVF
                     ASSTGGANAPDPSCSGNPRSCGMCGTGKPHSH"
     gene            1660..2472
                     /locus_tag="VG754_09225"
     CDS             1660..2472
                     /locus_tag="VG754_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWF19439.1"
                     /translation="MKFAICNEIFQGWKLEDAMAYAARAGYDAIELAPFTIAPLVTEI
                     SAAERRRIRESAARAGIGICGIHWVLVQAEGMYLNHSDASVRAHTAKYLCDLVDFCAD
                     VGGKAIVVGSPKQRNLLPGVTPSQAWAWAAETFRHSVECAQKRGVILCLEPLSPAETD
                     FINTAADAIRFVEQFRSPNFQIILDVKAMCSESQPIPDIIRASWPHFVHFHANDKNLK
                     GPGFGEVDFKPIAAALREVGYQGCVSVEVFKFEEGPEIIASKSIEYLKRSFA"
     gene            complement(2492..3571)
                     /locus_tag="VG754_09230"
     CDS             complement(2492..3571)
                     /locus_tag="VG754_09230"
                     /inference="COORDINATES: protein motif:HMM:NF015382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HWF19440.1"
                     /translation="MKKLTWILCLLVGGWLACSTASACTTFVMEGGGKIYFGRNLDWD
                     WESGLVFVNQRNISKTAVVAPDGVGAKWTSKYGSVTFNQFGLDVPFGGMNEAGLVVEN
                     MWLDETKYPAPDARPGINLAQWIQYQLDNCKTVKEVLATDNKVRLEPLAVPAGIHYLL
                     CDASGDCATVEFLDGKMAVHHGAKLPYHVLANDIYDSSAQFAKEHPGNSDGKPLRNTS
                     SFERFTRASECVADFKPRTSEQDVAYAFNALQQVRQGDSTVWQIVYDVSGRKIHFRTF
                     KNREERVIDFKALDFSCGHPLQFTDIESKPMAKGLEFKTLPVETLQKYLAAFYGQESL
                     KKMFGDLAPMIGIQLNALQNYQCAK"
BASE COUNT          903 a         1094 c         1054 g          922 t
ORIGIN      
        1 gttgtagcca acttcctgat aaatatattt gtggtccagg atgccggcat acccaggcgc
       61 cagccagtcg ctgaaccttt tgccgcaggt gcccgaaagc gacaggttgg ccgcgccgat
      121 gcagtaacaa tcccgacccc acgacatgat actgcgcaaa atctgatcaa gcgtttcgtc
      181 atcggtgctg accatccctc cttcgccggt ggtgatatgg tgggcgggat aaaaactaca
      241 gctcgacatc aaaccaaatg agcccaatgg ctttccccgc caggtacttc ccagcgcgtc
      301 gcaattatct tccagcagca acaggttgcg ttccttgcaa atgctcatga cacgatccat
      361 gtcgggcggg ttgcccagaa cgtgggcgaa gctgatcgcc tttgtactcg gtccgatggc
      421 cttctcaact tgatccaaat cgaggtttag cgatgcggga tcaatgtcca cgaacttggg
      481 cacaaatccg cattggatga tcggggcgac cgtcgtcgga aaacaaacca ctggggtgat
      541 gatttcgatg tccttaaatc cataagagcg gcgggattta atggtggaca tcatcagcaa
      601 attggccgaa ctgccggaat tgcacatcac cgaatatttt cggccaagat gtttcgaaaa
      661 ctgccgttcg aattgcgcga cattctcccc ggcgctcaac catttgccgc aaagaatggc
      721 aaacagagcc gcctcaagct cctcgttgtt ccaagtgggg ccggagtatt gcacgagagt
      781 ttttcccggc acaaaatttt gattatccaa tcttcgcatc gaagagattg gcagggatcc
      841 agccacaagt ttggagatta atttccgaat ttctatctct gtgagcatag cttctcgcaa
      901 cctagcgttc gcccttaacc aagtaaaggg tatatactct gtcaatcggc gcgccccggt
      961 acatcgtgac cctcttggga agaagagact ccaaagggac gggatggagc aggaatattc
     1021 tgggagaaat ttggggggct ttggtggcca ggatgtcggc ggggctttta tagccgcgcg
     1081 agcctttttt gcgggcggcg ttgcgggagc actcgtaaag gcgggtggca gccttgatgc
     1141 cgtctttgag ggcgcaggcc atgaggttgt ggcgctggga gaattgggga tcgtcagcga
     1201 ggaacatagt atggtaagtt tgggtgatgg ggagcggaat aaggtttgtc attactcctg
     1261 tttcacctga ggtgggcggc ttttgcagac gagtgccgcc tcaatgcgaa tgcggcttgc
     1321 cggtgccgca catcccgcag gagcggggat tgcccgagca ggaagggtcc ggagcgttag
     1381 cgccgcctgt gctggatgcg aagaccgaca gtttcttgga gagcttcgtc gagccgcaat
     1441 gcgggcactt ggtcccttcc cattcgcttg agcgcaccag gatttcgctg tccttctcgc
     1501 acttctcaca atgaaattcg tagatcggca tgggatgaaa ataatggcag cgccgggaat
     1561 ctcaagccgg aagtaaaagc gaaatcctgt ttttgtgatt cgtgaaaatt cgtgcaattc
     1621 gtgtctcact tgtgtttcat ctgcggctga tcgcatcaaa tgaaattcgc catctgcaac
     1681 gaaattttcc agggctggaa attggaagac gccatggctt acgcggccag ggcgggctac
     1741 gatgccatcg aacttgcgcc gttcaccatc gcgccattgg ttacggaaat ttccgcggct
     1801 gaacggcggc gcatccgtga atccgccgcg cgcgcgggca ttggcatctg cggcatccat
     1861 tgggtgctgg tgcaggcgga gggaatgtat ctgaaccaca gcgatgcttc tgtccgtgcc
     1921 cacactgcga aatatctgtg cgatctcgtg gacttttgcg cggatgtcgg tggaaaagcc
     1981 atcgtcgtcg gctcgccaaa gcaacgcaac ctgcttcccg gcgtgacgcc gtcgcaagca
     2041 tgggcgtggg ccgctgaaac tttccgccac tccgtcgagt gcgcgcaaaa acgcggtgta
     2101 atcctctgcc tcgaaccgct ttcgcctgct gaaaccgatt tcatcaacac cgccgccgac
     2161 gccatccgtt ttgtcgaaca atttcgcagc ccgaatttcc agatcatcct cgatgtgaaa
     2221 gcgatgtgct ccgaatcgca gccgatcccg gacatcatcc gcgcgtcgtg gccgcacttc
     2281 gtccatttcc atgcgaatga caaaaacctg aaaggccccg gtttcggcga ggtcgatttc
     2341 aagcccattg ccgccgcgtt gcgcgaagtc ggataccaag gctgcgtctc tgtcgaagtc
     2401 ttcaaattcg aagaaggccc tgaaataatc gccagcaaga gcatcgaata tttgaaacgc
     2461 agttttgcgt aacatcatcg gcatgctgcg atcacttcgc gcattgataa ttttgcaacg
     2521 catttaattg aatcccaatc attggcgcga ggtcgccaaa cattttcttc aacgattcct
     2581 ggccgtaaaa cgccgccaga tatttctgca acgtttccac cggcaaagtt ttgaattcca
     2641 aacctttcgc catgggcttt gactcgatgt ccgtgaattg caacggatgg ccgcatgaaa
     2701 aatccagcgc cttgaaatca atcacacgct cctcgcgatt tttaaaagtg cggaaatgaa
     2761 tcttccgccc tgacacgtca tacacaatct gccagactgt ggaatccccc tggcgcactt
     2821 gctgcaacgc attgaacgcg taagccacat cctgctcgga tgtgcgcggc ttgaaatccg
     2881 ccacgcattc cgacgctcgc gtgaaccgct cgaacgagga cgtgttcctc aatggcttgc
     2941 cgtccgagtt gcccggatgc tccttcgcga attgcgccga agaatcataa atgtcgttcg
     3001 ccaggacgtg gtaaggcagc ttcgcgccgt gatggaccgc cattttgccg tccaaaaatt
     3061 ccaccgtggc acaatctccc gaagcatcgc acagcagata atgtatcccc gcgggaacgg
     3121 cgagcggttc gaggcggact ttattgtccg tcgccagcac ctccttgacc gtcttgcaat
     3181 tgtctaactg atattggatc cactgcgcca ggttgattcc cggacgagcg tccggcgcgg
     3241 gatatttcgt ttcatccagc cacatatttt ccaccaccaa tcccgcctcg ttcatcccgc
     3301 cgaacggcac atccaagccg aactggttga acgtcacgct gccgtatttg gaagtccatt
     3361 tcgcgccgac gccgtccggc gcgaccaccg ccgttttgct gatgttccgt tgattgacga
     3421 acaccaaccc cgattcccag tcccaatcca ggttgcgccc gaaataaatc tttccgccgc
     3481 cctccatcac aaacgtggtg caggcagaag ccgtcgaaca ggccagccaa ccgccaacga
     3541 gcagacacaa aatccaagtc aattttttca tgattttaga cgctaaactg gtcgtcccga
     3601 taccacaagc cccgttttgg tagtttgaac ttggcttgac cgtttggaaa taatggcacg
     3661 acttgccttg actcgatttc ggacaaaaac ggtgtaatgc gttcatggag aatgggatgt
     3721 tccattttgc atggttttcc aaggcttgac tcccagaacc tcgcttttcc tctttgcgcc
     3781 tctgcgcctt tgcattgatg accgggccga aaaaattaat ttttttatgc ggaatcaagc
     3841 ggaatggggc ggaaataagc ggaattaagc ggaaatttct ccgtgacgat tgcggaaaat
     3901 acgtggatga aacgcaaagg cgtaaaggtg ccctgtgctc cgaaggagca ccggagattg
     3961 gcgagacacg cag
//
LOCUS       DASZMM010000380         5278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2902262, whole genome shotgun sequence.
ACCESSION   DASZMM010000380 DASZMM010000000
VERSION     DASZMM010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2902262"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VG754_09235"
     CDS             complement(<1..401)
                     /locus_tag="VG754_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19441.1"
                     /translation="MTLIGSPLSRLTGLVLVNGPMLPSLSNAMPAPDVAFVVQNDPRG
                     FAALGDAGAGSGNSLGYGAPSLIANSVALEFNIYTNAASGAGIALDNDGSIGPFTSTS
                     PVSLESGDPINVTLQYLNGLATVTLYDTVTS"
     gene            403..801
                     /locus_tag="VG754_09240"
     CDS             403..801
                     /locus_tag="VG754_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19442.1"
                     /translation="MQYLNGLATVTLYDTVTSNSFSTNAPVNVPSLVHGNQAYVGFTA
                     ADGGVLSTQTVSNFVYTSLIDLSVAQNGVNSLAVTWPVGVGGYVLQQDASLGDTNWVA
                     VTNAVSVVSGSNQVTVPLTSSNAFYRLVNP"
     gene            complement(953..2266)
                     /locus_tag="VG754_09245"
     CDS             complement(953..2266)
                     /locus_tag="VG754_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF19443.1"
                     /translation="MITAPSEQTGQPKAVRWYSGVTGYQWLVLAIASASWMFDTFEGQ
                     IYNLTRNQMLGEILHVAAGTAEIKKYGDILLAVFLAGGTAGGLLFGSLADKWGRRPTM
                     AVTILTYSLFSGLTFFATQLWQIAVLRFFVAMGVAGAWGAGASLIAEVFPPRARAQAS
                     GIFHATSVLGTWLATFAGLAVAAQWRYGYVLGILPALLILWVRFSIKEPASWQNASEK
                     AAEKAGPKMGSFSDLLLNPRWSKRALLGMLLAAIGLGSFWGVTVAGQDLTQEFLVKHG
                     MSAGEAAQHAKKAYGFIETAGGGLGLLAMGPLSALLGRRRAFQIMHVGAFLIVPVVCY
                     LPQTYWQLLLILPVFGFLTLGIHAGYAIYFPELFPTHLRATGTGFCFNVGRIVAASIL
                     WFSGVLKSLPGMDLRMAITLLGFLFPLGLLVIYFMPETKGQPLPE"
     gene            complement(2276..3025)
                     /locus_tag="VG754_09250"
     CDS             complement(2276..3025)
                     /locus_tag="VG754_09250"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWF19444.1"
                     /translation="MSSRAVEPIHALLITGGGTHDYAHQKNILTDGISARANVTWKII
                     YEDDAIKASGEDIKKHELAIYKEPDWTKGFDVVVHDECFGQVTNVAFIEHMAKAHYNG
                     VGAVVLHCSIHAYRLAKTDEWRKVLGVSSYHHETLRPFTVENLKPEHPVMKGFPATWQ
                     DFPDELYVIEKLWPECIPLAHGIGKKEGNNVCVWVNTYGKGRTFGTTLGHGNNTVESD
                     VYLDLVTRGLLWSCDKLGDDGKPKPGYGPAK"
     gene            complement(3133..4074)
                     /locus_tag="VG754_09255"
     CDS             complement(3133..4074)
                     /locus_tag="VG754_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWF19445.1"
                     /translation="MNSNQMPPRLRENSANANAGGLSRRNMLKLAGGGMLALGASAFA
                     GEAASDNKEYYKAKNGRINQSVIYWCFKPMPVPELAAYAARMGLKSVELVTPDFWPML
                     KENGLTCAIAPSHGFAKGFAHQSEHEECLNVLRQRIDECAAAGVPNVITFSGYRRGIS
                     DEEGIKNMVEGLKQVVGQAEKKKVTLCLEVLNSRVHIEMRGHPDYFADNLERGIEVCR
                     QIGSERMKILFDIYHIQIMEGDLITRIRQYHPYIAHYHTAGNPGRNEIDETQEINYPA
                     VMNAILATGYQGYVGQEFVPVRDAMASLSQAVKICDV"
     gene            4420..5208
                     /locus_tag="VG754_09260"
     CDS             4420..5208
                     /locus_tag="VG754_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF19446.1"
                     /translation="MKNSRFKAAFTLIELLVVIAIIAILAALLLPALARAKAQAIRTQ
                     CLSQNKQIALASLMYADDFGDHAVWPDWGVINAGWLYDPAGAGPPAPPSPDPTPAYVG
                     GVLYNYIGHNWHVYWCPADNTNSPNWIIRAERLSTYTMNGAVLAYHGKPVLGYPTHKI
                     VDMNPAAYMIWEPNSDNPTAAQIAYNDGANQPDQSDGPSDRHVTGCIVASYDGHAQLL
                     KTNIFLQQMELKVNSPGLLWADPDSKDGGGYNTSSTGNGCSLPQ"
BASE COUNT         1201 a         1508 c         1372 g         1197 t
ORIGIN      
        1 ttgctcgtta cagtgtcgta taatgtcact gtcgccagac cgttcaagta ttgcaaagtg
       61 acgttgattg ggtcaccgct ctcgagactg accgggcttg tgctggtaaa cggcccgatg
      121 ctgccatcgt tgtccaaggc aatgcccgcg ccggatgcgg cgtttgtgta aatgttgaat
      181 tccagcgcaa cgctgtttgc gatcagcgac ggcgctccat agccgagcga gtttccggag
      241 ccggcaccgg cgtcgcccaa tgccgcaaaa ccgcgaggat cattttgcac cacaaacgcc
      301 acatccggcg cgggcattgc gttggacaac gatggcagca tcgggccgtt taccagcaca
      361 agcccggtca gtctcgagag cggagatccg atcaatgtca cggtgcaata tctaaatggt
      421 ctggcgacag tgacattata cgacactgta acgagcaaca gtttcagcac gaatgcgccg
      481 gtcaatgttc cctcccttgt gcatggcaac caggcctacg ttggtttcac tgccgcagat
      541 ggcggtgtac tctcgacgca gacagtgagc aacttcgttt atacaagctt gattgatctg
      601 tcggtggcgc agaatggcgt caattcgctt gcagtaacct ggccggttgg tgttggcggc
      661 tacgtgctgc agcaggacgc gagtttgggc gacacgaact gggtggctgt caccaacgcg
      721 gtcagcgtcg tcagcggctc caaccaggtg acagtgccct tgaccagcag caacgcgttc
      781 taccgtctcg tcaacccgta agccttcgca catcttaaac gcaaaggcgg gagagaaatc
      841 tcccgccttt ttgttttatt tgatggtgct tgacaggatt tttttgcaaa atggactgaa
      901 cgaacctttt tccgttctcc ccctcacact ttcgagcgcg caagcgtggt gatcattcgg
      961 gcaacggctg gcccttggtt tccggcatga aatagatgac cagcagacca agcggaaata
     1021 agaatccgag cagcgtgatc gccatgcgca agtccattcc gggaagcgat ttcaacacgc
     1081 ccgaaaacca aaggatcgaa gcggccacga tacgcccgac attgaagcaa aatccggtcc
     1141 ctgtcgctcg cagatgagtg ggaaacaatt cgggaaaata aatcgcataa ccggcgtgga
     1201 tgcccagtgt gagaaatcca aataccggaa gaatcagcag gagctgccaa taggtttgcg
     1261 gcaaatagca aaccaccggc acgatgagaa atgcgccgac gtgcatgatt tgaaatgcgc
     1321 gccgcctgcc caaaagcgcc gacaacggtc ccatcgccag caaacccaaa ccgccgccgg
     1381 ccgtttctat gaaaccgtac gcttttttcg catgttgcgc ggcttcacct gcggacatgc
     1441 catgcttcac aaggaattcc tgggtcaaat cctgtcccgc aactgtcacc ccccaaaaac
     1501 ttccaagccc gattgcggcc aataacatgc ccagcaacgc gcgcttgctc cagcgcgggt
     1561 tcagtaacag atcgctgaaa cttcccatct tcggcccggc tttttccgcg gctttttcgc
     1621 tcgcgttctg ccagcttgcc ggttccttga tgctgaatct cacccacagg atcaacaacg
     1681 cgggaagaat tcccagcacg tagccataac gccattgtgc ggcaacggcc aaccctgcga
     1741 acgttgcaag ccatgtgccg aggacgctcg ttgcgtggaa aatgccagag gcttgcgccc
     1801 gcgctcgcgg ggggaaaact tcagcgatga ggcttgcgcc cgcgccccac gcgccggcga
     1861 cgcccatggc cacaaaaaat cgcaggacag ctatctgcca caactgcgtc gcaaaaaaag
     1921 tcagccccga aaaaagggaa tacgtcagga tggtcacggc catcgtcgga cgccgtcccc
     1981 atttgtccgc cagcgaaccg aagagcaatc ctcccgccgt gcctccggcc agaaaaaccg
     2041 cgagcaaaat gtcgccatat ttcttgatct cggcagtgcc ggcggcgacg tgcaggattt
     2101 cgccgagcat ctggttgcgg gtgagattgt agatctgtcc ctcaaacgtg tcgaacatcc
     2161 agcttgcgga tgcgatggcc agcacgagcc attgatatcc tgtcactccg gaataccagc
     2221 ggactgcctt gggctgcccg gtttgctcgc tcggcgcggt gatcatgaac ggcgattatt
     2281 ttgccggacc gtaaccgggt ttgggcttgc catcatcgcc caacttatcg caggaccaga
     2341 gcaaaccgcg cgtcactaaa tcgagataga catccgattc caccgtgtta tttccatggc
     2401 cgagggtcgt gccgaaagtg cggcccttgc cgtaagtgtt cacccaaacg caaacattgt
     2461 tcccttcctt tttgcctatg ccgtgcgcca gcggaatgca ttccggccat aatttttcga
     2521 tcacatacaa ctcatccgga aaatcctgcc acgtcgccgg aaaacctttc atgacaggat
     2581 gctcgggctt gagattttca accgtgaaag gacgcaacgt ctcatggtga tacgaactca
     2641 cgccgagcac cttccgccat tcatccgttt tcgcgaggcg ataagcgtgg atggagcaat
     2701 gcagcaccac cgctccgacg ccgttgtaat gcgccttcgc catgtgctcg atgaaagcga
     2761 cgttggtcac ctgcccaaaa cattcgtcat gcacgaccac gtcaaatcct tttgtccaat
     2821 ccggctcctt gtaaatggcg agttcgtgtt ttttgatgtc ttcgcccgat gccttgatgg
     2881 cgtcgtcctc gtaaatgatt ttccaggtca cattcgcgcg ggcgctgatg ccgtccgtga
     2941 ggatgttttt ctggtgcgca taatcatgcg tgccgccgcc ggtgatgagc agcgcgtgga
     3001 tcggttccac cgcgcgcgat gacattggga tttgtaacgc cgccacgcaa aatgcggaca
     3061 gaacgatgtg acgaagtgat ttgagtgagg tcatgtggtt tgattttagg gaataaaaat
     3121 gccgcggaca aattaaacat cgcaaatttt caccgcctgg ctcaacgaag ccatcgcgtc
     3181 gcgaactggc acaaattcct ggccgacata tccctgataa ccggtcgcaa ggatggcatt
     3241 catcaccgcc ggataattga tctcctgcgt ttcatcgatt tcattgcggc ccgggtttcc
     3301 cgctgtgtgg tagtgggcga tgtatggatg atattggcga atgcgcgtga tgaggtcgcc
     3361 ttccatgatc tggatgtgat agatgtcgaa aagaattttc atccgctccg aaccaatctg
     3421 gcggcagact tcaattcctc tttccagatt gtcggcaaag tagtcgggat ggcccctcat
     3481 ttcgatatgc acgcgtgagt tgagcacttc gaggcacaag gtcacttttt tcttttccgc
     3541 ctggccgacg acttgcttga ggccctcgac catgttcttg attccttcct cgtcggaaat
     3601 cccgcgccga taaccggaaa acgtgatgac gttcggcacg cccgccgcgg cgcattcatc
     3661 aattcgttgg cgcaatacgt tcaagcattc ctcgtgctcg ctttgatggg cgaaaccttt
     3721 cgcaaagcca tggctcggcg cgatggcaca ggtcagaccg ttctctttca acatcggcca
     3781 aaaatcaggc gtgaccagtt ccaccgattt caatcccatg cgcgccgcgt atgccgccaa
     3841 ttccggcact ggcatcggct tgaaacacca gtaaatgact gactggttga ttcgaccgtt
     3901 cttcgctttg taatattctt tgttgtccga tgcggcttcc ccggcaaaag cggacgctcc
     3961 caatgcgagc attccaccgc cggccaattt aagcatgttt ctgcggctca acccgccggc
     4021 gtttgcgttg gcggagttct cccgcaatct tggaggcatc tggttcgagt tcatgggcaa
     4081 attatatatc aggcatcaag tgcgacatag gcaaccgttt gtaatggaaa cgctttgcgg
     4141 tgcttaaaat tagatgctga ataaagcaag tttaccattg actatttatt ttggaagtga
     4201 aataattatt aaaatccatt gcaacccatg ctgacagcca tctgtaatca gccgaccttc
     4261 ggtcactctc ggtcgtattt tcatgccgaa cgcgcgtacg gaagccatgc cggttgtttg
     4321 aacaaaccgc atctgcaagc tattatctaa gaaagccata ttctcataat tcaaacgatt
     4381 tgcccggaaa accgggttgg ggaaaacgcc agtgatatta tgaaaaatag ccgattcaaa
     4441 gccgctttca cgctgattga actcttggtg gtcattgcca tcatcgccat tttggcggcg
     4501 ctgttgttgc ccgcgctggc gagggccaag gcccaggcaa tccgcacgca atgtctcagt
     4561 cagaacaagc aaattgcgct ggcttcgttg atgtatgccg atgattttgg cgaccacgcg
     4621 gtctggcctg actggggagt tattaacgcg ggctggcttt atgacccagc gggcgcaggt
     4681 ccacccgcgc cgccttcccc agaccccacg ccagcttatg tgggcggcgt gctttacaat
     4741 tatattggac ataactggca tgtctattgg tgcccggccg ataacaccaa ctcgcctaac
     4801 tggatcattc gcgcggaaag gctttcgact tacaccatga acggcgcggt tttggcatat
     4861 cacggcaaac cggtactggg ttatcccacc cacaagattg tggatatgaa ccctgcggct
     4921 tacatgattt gggagcctaa ctccgacaat ccaaccgcgg cgcagattgc ctataacgac
     4981 ggtgccaacc aaccggacca gagcgacggc cccagcgacc gtcatgtcac cggttgcata
     5041 gtggcttcgt atgatggtca tgcgcaactt ctgaagacca acatcttttt acagcagatg
     5101 gaattgaaag ttaattcacc cggcctgctt tgggccgacc cggacagcaa agacggtgga
     5161 gggtataaca ccagctccac gggcaacggt tgctcgctgc cgcagtgatg ccacagcgca
     5221 acgccacatt ctcctgccgc gttcaaaatt tcaccacgca accccggtca acggattc
//
LOCUS       DASZMM010000381         4755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1281570, whole genome shotgun sequence.
ACCESSION   DASZMM010000381 DASZMM010000000
VERSION     DASZMM010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281570"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VG754_09265"
     CDS             complement(<1..381)
                     /locus_tag="VG754_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HWF19447.1"
                     /translation="MIHPTAIIHPKAQLDSTVRVGPFAVIDEQVELGPHCVIGPHVYL
                     TGVTRIGAHNQFHAGCVIGDAPQDLKYKGEPTGLRIGEHNVFREQVTVHRSSKISDET
                     IIGSHNLLMVNSHVGHNSELGNHVI"
     gene            complement(488..1096)
                     /locus_tag="VG754_09270"
     CDS             complement(488..1096)
                     /locus_tag="VG754_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19448.1"
                     /translation="MVATLVFVLVFGAAAIITFILPKTYAATARMRLKSPPPQADAAT
                     TLTAQANAAQSEAVLNPVVESLKLNKIWGRKFRVDEDLKTWESVTLLKQRMEVLPAPA
                     SGELVVRVYDDDGELAAKIANRVGEILCSPSADSTGEIRAVLEMEMRAEPSVKPVQPN
                     IPLNLFVGAVGGFIIAWVAGSVAQWIATALKRQQQPTPKQTP"
     gene            1409..1675
                     /locus_tag="VG754_09275"
     CDS             1409..1675
                     /locus_tag="VG754_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF19449.1"
                     /translation="MSRTKFTHADETFRGLLREIRERKGLSQKAVAVRLGLPQSYISK
                     YELGERRLDFVETMVVCEALGTNLDSFLRLYLRRSVRPGKTAKA"
     gene            complement(1701..1868)
                     /locus_tag="VG754_09280"
     CDS             complement(1701..1868)
                     /locus_tag="VG754_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19450.1"
                     /translation="MDLSAIRKLAETLASFTVRVGGIWQTLRARIEKWAPLGYQDATG
                     FHVGIEEQSQD"
     gene            2040..2630
                     /locus_tag="VG754_09285"
     CDS             2040..2630
                     /locus_tag="VG754_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19451.1"
                     /translation="MNEWNIQSRGHACEACGKPFADKEQYHTLLFDEKQAFRRSDVCG
                     ACWERQFSEGARERKGFLSYWHGVYEAPVAAPEPIQKDTAESLLRKLIELNDPRYIPA
                     AYILAVMLERKRLLKVKEQLKRDGQRVFIYEHPKTGDLFTIIDPDLQLNQLEQVQRDV
                     ADLLEHGLNPPADTAAAVTTGDADAAPAPLSSDTEA"
     gene            2731..3438
                     /gene="rnc"
                     /locus_tag="VG754_09290"
     CDS             2731..3438
                     /gene="rnc"
                     /locus_tag="VG754_09290"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWF19452.1"
                     /translation="MADLKDLEARMGHVFRDADLLRLALTHPSVAHEQGTRIQHNQRL
                     EFLGDAVLQLILTRELYNQFPEVGEGPLTKARAKLVNRRSLAEKGKVLNLGEFLILSR
                     GEETHGGRERPSALADTFEAVLGAIFLDGGLVASEQFVQRHFGEAFGGLDVIPILENP
                     KGELQELLQSRSTQAPEYHVVSTSGPDHDRIFECTVHHEGKELARGSGKSKKAAESEA
                     ALAALNLLRGSPQINAD"
     gene            3539..3811
                     /locus_tag="VG754_09295"
     CDS             3539..3811
                     /locus_tag="VG754_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19453.1"
                     /translation="MRVKLENIFQIKFVPHDSYHIGEYYRSSGGDICSESWRLRQNIM
                     FEDELHYPEFPEDSIILDIGSTERGEELRAKLENLGFKLFKRTNHE"
     gene            4111..>4755
                     /locus_tag="VG754_09300"
     CDS             4111..>4755
                     /locus_tag="VG754_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWF19454.1"
                     /translation="MQIKYWRFELKLAHTWTVSTGVKTGGTNVSPVAILQLAADDGTI
                     GLGECAPASRYNENVDGGMEFLAKVDGSKLSFDDVRGSMRYVEGIAPGEFAAKSAVNI
                     ALLDGASRKAGKPIYDYYNLGFRDKYHVTSFSIGIDKPEVIRQKVLAAEKYPVLKLKV
                     GGPDDKANMKALREAAPKKWVRVDANEGWKTKEEALAMCEWLAKDGYIQYVEQPM"
BASE COUNT         1081 a         1252 c         1333 g         1089 t
ORIGIN      
        1 aatcacatgg ttgcccaact cgctattatg tccgacgtgg ctgttgacca tgaggagatt
       61 gtgcgagccg atgatggttt catcggaaat tttgctggaa cgatgcacgg tcacctgttc
      121 gcgaaagacg ttgtgctcgc cgatgcgcag gccggtcggt tcgcccttgt atttcaaatc
      181 ctgcggggca tcgccaatga cgcacccggc atgaaattga ttgtgcgcgc cgatgcgcgt
      241 cacgccggtc aaataaacgt gcggcccaat tacgcaatgc ggccccaatt ccacctgctc
      301 atcaatcacc gcaaaaggcc ccacgcggac ggtcgagtcc agttgcgcct tcggatgaat
      361 gatagcggtg gggtgaatca ttggaggcgg agagatggag tagtggagtg ttggagaaat
      421 ggaactgaca aacccgaaat gtccagcatc cagcatccag cacccagcat cttcagtttg
      481 aaaagtttca tggcgtctgt tttggcgttg gctgctgttg ccgcttcaac gccgtcgcaa
      541 tccattgtgc cacgcttccg gcgacccacg cgattatgaa tccgccgaca gcgccgacaa
      601 agaggttcaa tggaatattt ggctgcaccg gtttgacgct cggctccgcg cgcatctcca
      661 tctccagcac cgcgcgaatt tcgccggttg aatctgccga aggactgcaa aggatttccc
      721 ccacacggtt ggctatcttc gccgccagtt cgccatcatc gtcataaaca cggaccacaa
      781 gctcccctga agccggcgcg ggcaaaacct ccatgcgctg tttcagcagg gtcacgcttt
      841 cccaagtttt caaatcttcg tccacgcgga attttcttcc ccaaatcttg ttcagtttca
      901 aactttcgac caccgggttc aacacggctt ccgattgcgc ggcgttcgcc tgcgcggtga
      961 gtgtcgtggc ggcatcggct tgcggcggcg ggcttttcaa gcgcatccgc gccgttgctg
     1021 cgtaagtttt tggcagaatg aacgtgatga tcgcagccgc gccgaacacc agcacaaaaa
     1081 ccagcgttgc caccatcagg aagactttcc ccggttttct cgccgatttg tcaggtaaaa
     1141 tatttcggtt cgatgaactc aatcttgtct ttattgttca ggattgcggc cgttagtata
     1201 gcaaaaattt ccatgggtga tgtttacaat gatcccgaaa atgcccgaga aggcttccat
     1261 gccatgaaat tcacgagcat ggctttggac ccggtaacaa catgtttgac ttgcttcttt
     1321 tggggtgaaa gacaccccgg gtgcggcggg gctgttttta gattatccca atttgggttt
     1381 ttccatgtaa atctgcaatt atagcgccat gtcgaggacg aaatttaccc acgcggatga
     1441 gacgtttcgc gggctcctcc gggagatacg cgagcgcaag gggctctcac aaaaggccgt
     1501 ggcggtgcgc ctgggtttgc cccagagtta tatcagcaaa tatgaactcg gtgaacggcg
     1561 cctcgacttt gtcgagacaa tggtcgtgtg cgaggcgctg gggacgaact tggacagttt
     1621 cctgagatta tacctgcgca ggtcggtcag gccgggcaaa actgcaaagg cataaacccg
     1681 tcttgggttc gtgccgttgt tcagtcttgc gattgctctt cgatgccgac atggaatcct
     1741 gtcgcgtctt gatagccaag tggcgcccac ttttcgatgc gcgcgcgaag agtttgccag
     1801 attccgccga cacgcacggt gaagcttgca agtgtctcgg ctaattttct gatggcgctc
     1861 agatccataa tggtcctgtt ggttcggcga tggttcaatc ataggcagat gccaattttg
     1921 gtcaacagga cgaattcctt tttcgatgtc ggggaatgtc ctacgcggca tgattcaggt
     1981 tgaagtgccg ccggagttgg gcatagtatt ttccagcgca ttgatggcgc atcaatttga
     2041 tgaatgagtg gaacatccag tcacgcggcc atgcctgcga agcctgcggc aaacctttcg
     2101 ccgacaagga gcagtatcac acgctgttgt tcgacgagaa acaggcgttc cggcgttccg
     2161 atgtttgtgg cgcctgctgg gaacggcaat tcagcgaagg agcgcgcgaa cgcaaaggct
     2221 tcctctccta ttggcacggt gtttacgaag cgccagttgc cgcgccggaa ccgatccaaa
     2281 aggacactgc ggaatctttg ctgcgaaaat tgatcgagtt gaacgacccg cgttacattc
     2341 ccgccgctta catcctggcg gtcatgcttg aacggaaacg gttgctcaag gtgaaggaac
     2401 agttgaagcg cgacggccag cgggtgttca tttacgagca tcccaaaacc ggcgacttgt
     2461 tcaccatcat tgatccggat ttgcaactca accagctcga acaggtgcaa cgggacgttg
     2521 ccgacttgct ggaacatggc ttgaatccac ctgcagatac cgctgccgcc gtcaccacag
     2581 gcgacgcgga tgccgcaccc gctcccctct cgtccgacac ggaagcgtag aataccgatg
     2641 acgccgctgt caccgtcagt tgacccggca tcacgctgag gcttgaaatt cgcttcggct
     2701 ttgggtgttg ggcatgggac ttaggaattt gtggctgact tgaaagacct cgaagcccgg
     2761 atgggccatg ttttccgcga tgctgatttg cttcggctcg cgttgacgca tccatccgtc
     2821 gcgcacgagc agggcacgcg catccagcat aatcagcggc tggaattcct cggtgacgcc
     2881 gtgctgcaat tgattctcac gcgcgagttg tataaccaat tcccggaagt cggcgaaggc
     2941 ccgctcacca aggcgcgcgc caagctcgtc aaccgccgtt cgctcgctga aaaaggaaag
     3001 gtcttgaacc tgggcgaatt tctgatcctc agccgcggcg aggagacgca cggcggacgc
     3061 gaacggcctt cggcgctggc ggacacgttt gaagcggtgc tcggcgcgat ttttctcgat
     3121 ggcggtctgg tcgcctcgga acaatttgtg caacggcatt tcggcgaggc ttttggcggg
     3181 ctggacgtca ttccaatcct cgaaaatccc aagggtgaat tacaggaatt gcttcaatcc
     3241 cgctccaccc aggcgcctga atatcacgtc gtctcgacca gcgggcccga ccatgaccgc
     3301 atctttgaat gcacggtaca ccatgaagga aaagaactgg cacgcggcag cggcaagagc
     3361 aaaaaagccg ccgaaagcga agcagcgctg gctgcgctca atttgttaag aggttcaccg
     3421 cagataaatg cagattaacg cagatgggga taaatcgggg ccttttatac gaaattcttt
     3481 tgctcagaca atgaagacat cccattcata tttcttcgat tccaaggaca ttgagtctat
     3541 gagagtgaaa cttgaaaaca tttttcagat taaatttgtg ccgcatgaca gctatcacat
     3601 tggtgagtac tatcgctctt cggggggtga tatctgcagc gaaagctgga ggttgcgcca
     3661 gaatattatg tttgaggacg agttgcatta tcctgaattt ccggaggaca gcatcatact
     3721 ggatatcggc tcgacggaac gtggagaaga attgagagcc aaactcgaga atcttggatt
     3781 taaacttttt aagcgaacga atcatgaata gtccgaaaca tcacgacgct ttccgattgc
     3841 cctgccccga gaacttcgat agctcacttc cccatcaccc gggctctctc gcttggggag
     3901 agaattggcc gaaacggaat tttcgcgctt tgaacccctg aaccgtgcga ctggcgaaag
     3961 tcttctccct gcctccatcg agaggactgg ggtgaggggg accgcgattc tcgtcaatcc
     4021 atccctctca gcgaatccag ttggcgtaga taagcccgtt cttaacagaa cgagattgcc
     4081 tcggctcgcg ttttgcgctt tggttggcgg gtgcaaatta aatattggcg ttttgaattg
     4141 aaactcgcgc acacttggac ggtttcgacg ggagttaaga cgggcggcac gaatgtttct
     4201 ccggtggcca tcctccaact cgctgcggat gacggaacca tcggcctcgg cgaatgcgcg
     4261 ccagcaagcc gttacaatga aaacgtggat ggcggcatgg aatttctggc aaaggtggac
     4321 ggcagcaaac tttctttcga cgacgttcgc ggaagcatga gatacgtcga gggcattgcg
     4381 ccgggagagt tcgcggcgaa gtcggcagtg aacatcgccc tgctcgatgg cgcgtcgcgg
     4441 aaggccggca aaccgattta cgattattat aatcttggtt ttcgcgacaa atatcatgtg
     4501 acctcgttca gcattggcat tgacaagccg gaagtgattc gtcagaaagt tttggcggcg
     4561 gaaaagtatc cggtgttgaa actgaaagtt ggcgggccgg atgacaaggc gaacatgaag
     4621 gcgttgcgtg aagccgcacc gaaaaaatgg gtgcgcgtcg atgccaatga aggctggaaa
     4681 accaaggaag aagcgcttgc catgtgcgaa tggctcgcga aagacggcta catccaatac
     4741 gtcgagcaac cgatg
//
LOCUS       DASZMM010000382         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1801501, whole genome shotgun sequence.
ACCESSION   DASZMM010000382 DASZMM010000000
VERSION     DASZMM010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801501"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1557)
                     /gene="rhaB"
                     /locus_tag="VG754_09305"
     CDS             complement(73..1557)
                     /gene="rhaB"
                     /locus_tag="VG754_09305"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HWF19455.1"
                     /translation="MSDQIYLAVDLGAESGRVMAGLWDGKQMRLEQVHRFPNEPVEMG
                     DGLHWDIARLWLEIQNGLASAARNYGNRIISVGVDTWGVDHVLLSKNSELLGQPYHYR
                     DRRTRGVMQAAFSRVPRASIFAETGLQFMEFNTLYQLLALQKHAPEILAAADRLLMIP
                     DYLNWCLCGARVGEFTNASTTQMLRPDGTWSRELLEKFNLPANIFPEIVRPGTRLGKL
                     RESVAERAGLKDVAVIAPATHDTGSAVAAVPTAHTGSANWAYLSSGTWSLMGVEMQQA
                     QLSPRVLELNLTNEGGVDGTFRLLKNIMGLWLVQQCKRSFEARGKKFDYASLMQLAAA
                     ERPLVSLVNPNDARFLNPPDMPRAIQDFCRETGQPAPESEGALIRCALESLALIYQHV
                     LRGLEEVTGASIEVIHIIGGGSQNEVLNQFTADACGRPVLAGPVEATALGNLLMQARA
                     CAEIGSLAELRAVVTNSSEVRRFEPNAKNAEKWHEASLHFSKII"
     gene            complement(1748..2200)
                     /gene="rpiB"
                     /locus_tag="VG754_09310"
     CDS             complement(1748..2200)
                     /gene="rpiB"
                     /locus_tag="VG754_09310"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HWF19456.1"
                     /translation="MKTIVIGSDHAGFELKQTIKKFLAHDHHIIDVGAHRELRTDDYP
                     DYAEKVGRAVLEERGRRGILICGSGVGASVAANKLKGIRAAVCHDSYSAHQGVEHDDM
                     NVLVLGSRVIGTALAHDLVVAFLGAKFTREKRHVRRLAKIALLEEKRL"
     gene            complement(2252..2953)
                     /locus_tag="VG754_09315"
     CDS             complement(2252..2953)
                     /locus_tag="VG754_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19457.1"
                     /translation="MDRSEAKEVLARYRPGETFGDPQVGEALELARRDPQLAEWFAKQ
                     RAAHVAGTQVLPEVAPAQPERKPFISKPALAIFFIVVALLLLMLIWTLNTPKPKDPFI
                     NYRDRMVRLVYRAYPMQINFSDQAQIRDYFRTNAGPVDYSLPRNLEKLPAKGCAMFTW
                     ANHPVAMMGFDAGGNTNLSLFLISRSAFDDTPVPAAPEYALIRKLLTVSWTSGGRVYL
                     LAGPNDPALLRNHLE"
     gene            3114..3347
                     /locus_tag="VG754_09320"
     CDS             3114..3347
                     /locus_tag="VG754_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19458.1"
                     /translation="MYIEYRNFKCNRARTAKVIKTVDDKSKIVRKTIQVDTWITVNFT
                     VVPQIALPYRNQNKRFESTGVLRLVHHRRFTIR"
     gene            3437..>3603
                     /locus_tag="VG754_09325"
     CDS             3437..>3603
                     /locus_tag="VG754_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWF19459.1"
                     /translation="MSGLIAIVGRPNVGKSALFNRIAGRRIAIVHDQPGVTRDRVSAE
                     VEWSGRPFTLV"
BASE COUNT          807 a         1002 c         1015 g          779 t
ORIGIN      
        1 ggtcgcagtt tgcaatcccg cctccccgaa cagctctaaa tcagagggcg gttcgtgatt
       61 catgcgcccg ggtcagatta ttttggagaa atgaaggctg gcctcgtgcc acttctccgc
      121 gtttttggca ttcggctcga atcggcgcac ttcggatgag ttggtgacca ccgcgcgcaa
      181 ttcggcaagt gaaccaattt ccgcgcacgc acgcgcctgc atcagcagat tgccaagcgc
      241 agtcgcttcc acaggaccgg cgagaacggg ccgcccgcaa gcatccgcag taaattggtt
      301 cagcacttcg ttttgcgaac cgccgccgat gatgtggatc acttcaatgg acgcgccagt
      361 cacttcttcg agtccgcgga gaacgtgttg ataaatgagc gcaaggcttt cgagggcgca
      421 gcggataagc gcgccttcgg attcgggcgc gggctggccc gtttcgcggc aaaaatcctg
      481 gatggcgcgc ggcatgtcgg gaggattgag aaaacgcgcg tcgttgggat tgacgagcga
      541 gacgagggga cgttccgcag cggcaagttg catgaggctg gcgtaatcga atttttttcc
      601 gcgcgcttca aaggagcgtt tgcattgctg cacgagccag aggcccatga tgtttttcag
      661 caagcgaaac gtgccgtcca cgccgccttc attggtcaga ttcaattcca gaacgcgcgg
      721 tgagagttgc gcctgttgca tctccacgcc catcagcgac cacgtgccgg aactgagata
      781 ggcccaattc gccgagccgg tgtgcgcggt gggaacagcg gcaaccgccg agccggtgtc
      841 atgagtcgcg ggcgcgatga cggcaacgtc cttcaacccg gcgcgttcgg cgacggattc
      901 gcgcaatttg ccgaggcgcg ttccgggcct gacaatttcg gggaaaatgt tcgcgggcag
      961 attgaatttt tcgagcagtt cgcgcgacca cgtcccgtcg ggtcgaagca tttgcgtggt
     1021 gctcgcgttg gtgaattcgc cgacgcgcgc tccgcagagg caccaattca aataatcggg
     1081 aatcatcagc aggcggtcgg ctgcggcgag gatttcaggc gcgtgttttt gcagcgcgag
     1141 cagttgataa agcgtgttga actccatgaa ttgcaggccg gtctcagcga agatgctggc
     1201 gcgtggaacg cggctgaacg ccgcttgcat cacgccgcgc gtgcggcggt cgcgataatg
     1261 gtacggttgg ccgagcaatt cggaattttt tgagagcaac acgtgatcca cgccccacgt
     1321 gtccacgccg acggaaatga tgcgatttcc gtaattgcgc gcggcgctgg caaggccgtt
     1381 ctggatttca agccaaagcc gggcgatgtc ccaatgcaaa ccatcgccca tttcaaccgg
     1441 ctcatttgga aagcgatgga cttgctccaa acgcatttgc tttccatccc aaagaccggc
     1501 catgacgcgg ccgctttccg cgccaagatc aacagcaaga taaatttggt cgctcacgtt
     1561 ttaaataccc aaacacccaa tgtagaaaca ccaaagaaat ccaaaatccc aagctcccat
     1621 atctgacaga atgaacagaa ttttaagaat gaaaatacaa ctttaggaaa ccattccatt
     1681 ctgaagattc tgttcattct gtcgaaaaaa tgtggcagcc gaaccttatt ctcgtcggtt
     1741 tagtgtttta cagccgtttc tcttccaaca acgcaatttt cgccaacctt ctcacatgcc
     1801 gtttttcgcg cgtgaatttt gcgccgagaa acgccaccac caaatcgtgc gccaaagcgg
     1861 tgccgatcac ccgcgagccg agcacaagca cgttcatatc gtcgtgctcc acgccttgat
     1921 gcgcggagta ggaatcgtgg caaacagcgg cgcgaatgcc tttcaatttg ttcgccgcaa
     1981 cggacgcccc gacgccgctg ccgcaaatca aaatgccccg gcgtccgcgc tcttcaagaa
     2041 ccgcgcggcc aactttttcg gcgtagtcag gataatcatc ggtccgcaat tcgcggtgtg
     2101 cgccgacatc aatgatgtga tgatcatgcg ctaggaattt tttgatcgtt tgcttcaatt
     2161 caaagcccgc gtggtcggag ccgatgacga tggttttcat ggaatttgcg atttacgata
     2221 tgccatttac ggctgcgcgg aaggcaggtg ttcattccag atgattccgc agcagcgccg
     2281 gatcattcgg tcccgccagc agataaaccc ggcctcccga cgtccaactg acggtcagga
     2341 gtttgcggat gagggcatac tcgggagcgg caggaacggg agtgtcgtca aaggcggagc
     2401 gcgagatgag aaagagggaa agatttgtgt tcccgccggc atcaaagccc atcatcgcca
     2461 ccggatgatt ggcccaggtg aacatcgcgc agcctttcgc cggaagtttt tcaagattgc
     2521 gcggcaagga ataatccacc gggccggcat tggtgcggaa ataatcgcgg atttgcgcct
     2581 ggtcggagaa gttgatctgc atcgggtaag cgcggtaaac aagccgcacc atccggtcgc
     2641 ggtagttgat gaacgggtcc ttcggtttgg gtgtgttcag ggtccaaata agcatcaaca
     2701 gcaaaagggc cacgacgata aaaaagatcg cgagcgcggg tttgcttatg aaaggcttgc
     2761 gctcgggttg cgcgggcgca acctcgggca acacttgggt accggcaacg tgagcggcgc
     2821 gttgcttggc gaaccattcg gcaagctgcg ggtcgcgacg cgcgagttcc agcgcttcgc
     2881 cgacctgtgg atcaccaaag gtctcgccgg gccgataccg cgccaatact tccttggctt
     2941 cgcttctatc catggaattc caaatcgctg cgcattttca tgcaaatagg aaaataattc
     3001 tctgtgctca atttcaaatc accccgcatc aaaacacaag ttttgcgccg ttctgacata
     3061 aaggcgagtc aaaagcggcg ggggaacatt atagccgcct tgacctctta cccatgtaca
     3121 tcgaatatcg caatttcaaa tgtaaccgcg cgagaactgc aaaggtcatc aaaactgtgg
     3181 acgacaaatc gaaaattgtc cgtaagacca tccaagtgga tacttggatc actgtgaatt
     3241 tcacagtagt gccgcagatt gccttgccat atcgcaatca aaacaagcgg tttgaatcca
     3301 cgggagtctt gcgcctcgtc catcaccgcc ggttcaccat ccgttgacag gttaccccct
     3361 ctactcccgt cggctgttgg ccttcttcat tcttttaact ttcgcgtcgc ctcccggtgc
     3421 ggcatggtga agtgatatgt ctggattaat tgccatcgtt ggccgtccga acgtggggaa
     3481 gtccgcgctg ttcaatcgca tcgcgggacg gcgcatcgcc atcgtccacg atcaaccggg
     3541 cgtcacccgc gatcgcgtca gcgcggaagt cgaatggagc ggacgcccgt tcacgctggt
     3601 gga
//
LOCUS       DASZMM010000383         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4005278, whole genome shotgun sequence.
ACCESSION   DASZMM010000383 DASZMM010000000
VERSION     DASZMM010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4005278"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1341)
                     /gene="tsaD"
                     /locus_tag="VG754_09330"
     CDS             complement(316..1341)
                     /gene="tsaD"
                     /locus_tag="VG754_09330"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWF19460.1"
                     /translation="MTLLALETSCDETSVAVVRDGKVLSSLVSSQIKLHAEYGGVVPE
                     LAAREHLRNLMPVARAALDQAQISINALDAIAATQGPGLPNALLLGLKAAQAVAFALR
                     KPFLGIHHHEAHLYSPWIAGDPPAADFANFQPNISLVVSGGHTLLVHVKSELEHLVLG
                     STIDDAAGECFDKVAKLIGLAYPGGPEIDRLAAKGNADKFDFPRPMLRDASDDFSFSG
                     LKTSVRYFLRDHPETLETETAINDLCAGVQAAIVEVLVGKTIRAAQRLGVSCITASGG
                     VTCNRALRAALEKACAQKNLTLRLANRNLCTDNAAMIGILAERKFLHGAPPTSLDAEI
                     MPGWALA"
     gene            complement(1425..2774)
                     /locus_tag="VG754_09335"
     CDS             complement(1425..2774)
                     /locus_tag="VG754_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWF19461.1"
                     /translation="MKRRFVYALIGVLLAANLTIGGRIYFRSAQAAEKDSAYPSLELF
                     SYVLEKVRKDYVDGKNLTYQELVYGALKGMINTLDPHSEFMDPAKYDELQKDTQGAFG
                     GLGIMIEMKDHYVTVLAPMDDTPGFKAGVLSGDRILKIDGKPVEQLGAQDAERLQESV
                     QHLRGEPGTEVTVTFERPSTGAVKEMKLTRANIKVDMVKDINNKKEFPLAEDKIGYVR
                     LVQFGEKTSDDLEKALKKLKAQGMQGFVLDLRRNPGGLLDQAVEVCEKFLPRNAMIVS
                     TEGQNPSQNSKRYSNGRGDELDGMPMVILVNVNSASASEIVAGCLQDYHRAIILGERT
                     FGKGSVQSILPLQDGSALRLTTAKYYTPSHKVIHGQGITPDDPVPMTEDEELAVVLKT
                     RPGLDTLTPKQQEELRNAHDVQLDRATDLLHGILIYARRSGSNQKMAEKDGKEVAEK"
     gene            complement(3127..>3394)
                     /locus_tag="VG754_09340"
     CDS             complement(3127..>3394)
                     /locus_tag="VG754_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWF19462.1"
                     /translation="DRSKLWAVQTPQTFRVEIIRRALSVVREKGLQVTDDTAACELIG
                     QPVRLVAATTPNPKVTVPADLPYVELLLNTQIPNHPSAQTNAKP"
BASE COUNT          790 a          912 c          895 g          797 t
ORIGIN      
        1 aaacgccgcc atgttcctca aacgcacgga ccgtgaatct gccgcgatag ccggtgttca
       61 atgaatgtcg aatctcgctt cagcttgaca agtcgcccgc gatagaatct tttctttttg
      121 tgagaaacgt ggcacacaaa tttaagatta agagccgccc aatgctcctc aaggcgggat
      181 taactcacac tgcgctcaaa aaattggccg gcgatctgga agtaaaaacg tttttggaaa
      241 aaaccaggcg tttgcaagac ccgacaaaag aggcctcatc tcgggacaga ccaaaaaaat
      301 aatcgggaaa tgccgtcatg ccagcgccca gccgggcatg atttcggcat cgagagaagt
      361 cggcggcgcg ccatgaagaa atttgcgctc ggccaaaatg ccgatcatcg cggcgttgtc
      421 agtgcaaaga ttccggttcg cgaggcgcaa ggttagattt ttttgggcgc aggctttttc
      481 aagcgcggct cgcaaagcgc gattgcatgt cacgcctccc gatgcagtga tgcaagaaac
      541 gccgagtctc tgcgcggcgc gaatcgtttt tcccaccagg acttctacaa tggcagcctg
      601 cacacccgcg cacaggtcgt tgattgccgt ttcagtttct agcgtttcgg gatggtcgcg
      661 aaggaaatag cggacggaag ttttcaatcc gctgaagctg aaatcatcgc tcgcatcgcg
      721 caacatggga cgcggaaaat cgaacttgtc ggcgttcccc ttcgcagcga gccggtcgat
      781 ttccggtccg cccggatacg caagcccgat cagcttggcg accttgtcga aacattcgcc
      841 cgcggcatcg tcaatcgttg agccgagcac gagatgttcg agttcggatt tcacatgcac
      901 cagcaacgtg tgaccgccgc tgacgacgag cgaaatattt ggctgaaaat tcgcaaaatc
      961 ggctgcgggc ggatcgccgg caatccatgg cgaataaaga tgcgcctcat gatgatggat
     1021 gccgagaaac ggcttgcgaa gcgcgaaagc caccgcttgc gcggccttca acccgagcag
     1081 caatgcattc ggcaaaccgg gtccttgcgt ggcggcaatg gcatcgagag cgttgatgga
     1141 aatttgcgcc tggtccaatg ccgcgcgagc gaccggcatc aagtttcgca aatgttcgcg
     1201 cgcagccaat tccggaacca ccccgccata ttcggcgtga agttttattt gggaggagac
     1261 aaggctggat aaaaccttgc cgtcgcgcac aacggccacg ctggtttcat cgcaggacgt
     1321 ttcgagggcg agaagggtca tttaaggttt gaagacaaca tccaacatcg aacgttgaac
     1381 actgaatgtt aactgaattg gacgttcgac gttcgatgtt tttcctattt ctctgcgact
     1441 tctttgccgt ctttctcggc cattttttga ttgctgcccg aacgacgggc gtaaatcaaa
     1501 atgccatgca acaaatctgt cgcgcgatcc aactgaacat cgtgcgcgtt gcgaagttct
     1561 tcctgctgct tgggcgtcag cgtgtcgagg ccgggacgcg ttttgagaac gactgccaat
     1621 tcctcgtcct cggtcatcgg cacgggatca tcgggtgtga tgccctggcc atggatgacc
     1681 ttgtggcttg gcgtgtaata ttttgccgtc gtcaggcgca atgccgaacc atcctgcaac
     1741 ggcagaatgc tttgcaccga gcctttgccg aaggtccttt cgccaagaat gatcgcgcgg
     1801 tgataatctt gcaaacagcc ggcgacgatt tcagaagcgc tggcgctgtt gacattgact
     1861 aaaatcacca tcggcatgcc gtcgagttca tcgcctcggc cattggagta acgcttggaa
     1921 ttttgcgaag gattctgtcc ctcggtcgaa acaatcatcg cgttgcgagg aaggaatttc
     1981 tcgcacactt cgaccgcctg atcgagcaag ccgccgggat tgcgacgcaa atccaggacg
     2041 aatccctgca tcccctgcgc tttcaacttc ttcaatgcct tctcaagatc gtcgctcgtt
     2101 ttttcgccga actgcacaag gcgcacataa ccgatcttgt cttcggcaag cggaaattcc
     2161 ttcttgttat tgatgtcctt gaccatgtcc accttgatgt tggcacgggt caatttcatt
     2221 tccttcaccg cgccggtcga aggccgctcg aaagtaactg tcacttccgt gcccggttcg
     2281 ccgcgcaaat gttgcaccga ttcctgcaaa cgctctgcgt cctgcgcgcc gagttgctcc
     2341 acaggtttgc cgtcaatctt gagaatgcgg tcgcccgaaa gcacgcccgc cttgaaaccg
     2401 ggcgtatcat ccatcggcgc aagcacggtg acgtaatggt ccttcatctc gatcatgatg
     2461 ccgagaccgc cgaacgcgcc ctgggtgtcc ttttgcaact catcgtattt ggcgggatcc
     2521 atgaattcgc tgtgaggatc gagcgtgttg atcatcccct tcaacgcgcc atagaccagt
     2581 tcctgatagg tcagattttt gccatcgacg taatccttgc gcactttttc aagaacatag
     2641 gagaacaatt cgaggctcgg atacgcggaa tccttctccg cagcctgggc ggaccggaaa
     2701 taaatgcgtc cgccaatggt caagttggcc gcaaggagca ccccgataag agcgtaaaca
     2761 aagcgtcgtt tcataaaaat tccaaatacg tcacgctgac aataaatggg ctgcgacgct
     2821 tttgcaagga agggaaacgg gaaaattacc caagaaatcg gaaatcctaa actttgatat
     2881 ctgacagaat caacagaata ttcagaatgg aatattttgc gttcattcgg tcttagccgt
     2941 aacgatgcag ttgagccacg gattgaacac tgatgaaaca cggatgggag cggtgaacag
     3001 ccattttggc ggttcaaaaa gcactcacca aagctgatca cctttcctgc cttgaaaaat
     3061 ggcttctgcc tgcatttctc cgtgttcaat ccgtgttcat ccgtggctga attcaatggt
     3121 tacggcttag ggcttggcat ttgtttgggc acttgggtgg ttgggtattt gggtatttaa
     3181 tagaagttcc acatagggca aatccgccgg caccgtcact ttgggattgg gtgttgttgc
     3241 ggcaacaagc cgtaccggtt ggccgatcaa ttcgcaagct gccgtgtcat cagtcacttg
     3301 caaccctttt tcgcggacaa ccgaaagcgc gcgccggatg atttccacgc gaaatgtttg
     3361 cggagtttgc actgcccaaa gtttcgaacg gtcc
//
LOCUS       DASZMM010000384         8737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3459872, whole genome shotgun sequence.
ACCESSION   DASZMM010000384 DASZMM010000000
VERSION     DASZMM010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3459872"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VG754_09345"
     CDS             <1..484
                     /locus_tag="VG754_09345"
                     /inference="COORDINATES: protein motif:HMM:NF018963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HWF19463.1"
                     /translation="RLHYTTDWFVDNEQKGVVKILAPELPGAEPFTQKVGIMSQHPEH
                     YRQLKSHPELIPAIKAAEDRINAFSLKYVPMDKLKAAEPLLVTGDIVGVATTEEGIDI
                     AHTGLCIKDEQGVVHFMDASSSRRNMKVTLEREISACLNWSPKLTGAMFARPLEVGKV
                     "
     gene            1066..2250
                     /locus_tag="VG754_09350"
     CDS             1066..2250
                     /locus_tag="VG754_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19464.1"
                     /translation="MHAQLRVTADNAYTVYLDGRELGRGSDWRTLSAYDLSWLLQPGP
                     HVLAVDAFNDVNKAGVIAGLHAELSDGRTIEIKSDTNWLVVPLTDLDWLTKRKASRDW
                     PHAIVVGLLGEGPWWQPTPTEIANIAPLFPLKTHFWENGWFQITLLSFCVLTLITSAR
                     LMTQLAVQARAGRLLQSERARIARDIHDDLGARLTKLLVTGEEAQDRLLEQADARTNV
                     DQICDGARDILNAIDEIVWVVNPQRDKLNDSLIYICKYAESFLRAASVRCRFDIQSDM
                     PEISLDLPFRRNIFLTIKESLSNAVKHSGATEVSIQVRLDGPTLRALVEDNGHGFDPS
                     QINTTRHGLSNMIARIKDLGGNCRVESKPGTGCRVAFSVPLPLARSQQWVVKWWYGLS
                     SR"
     gene            2366..3046
                     /locus_tag="VG754_09355"
     CDS             2366..3046
                     /locus_tag="VG754_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF19465.1"
                     /translation="MKQQPAIEPVKSTRKSPFRVAVVEDEQLFRESWARRINSFPDFK
                     CLCECASGEEALAMVPGKQPDVILMDVLLPGISGIECTFRLKKLLPESLIVILTALDD
                     DELIFRALEAGADGYLLKRTKPVDVRAALLDVLSGGAPMTSAIARRVVKFFRKEPVAK
                     QESIRLSAREEEMLELLSKGYSNKEIAEQSHLTLETVRTYLKRIYEKMHVRSRTEAAV
                     RYVHFKSP"
     gene            3223..6684
                     /locus_tag="VG754_09360"
     CDS             3223..6684
                     /locus_tag="VG754_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWF19466.1"
                     /translation="MKKTTLLPSNPLLWGIALALVLFYSQTARAGLGIPYSQDADTMH
                     LWHLNDATNQLYAVDSATNPVDGAIPIQLENIGEPTPGQQPTNALYGSPGPGYPGLGS
                     CYSGSTKQHVLAGYNGSSYGGDFPDVSQFCNPNTGAFTFEALINLGSGSSPLSSVDAE
                     ILSGDNNGGLGNRGWQWRIEGGTMEWDLLAGSTDNDFKAPLPTSGPNAVKLNAWFHVA
                     VTFTGQSPTNGDPANVFTMYWTALDPSRTSADVLAQYTNDVASPYGIRPLSGAPEGTA
                     TPALGISGSSRNTSSNPGNNEGMIGSIMELRVSDIARQSNQMAFVAGGSFPPAITQQP
                     PTNTLVGYGQPLVFGPTASGTAPLQYEWFQGGTNLASQTNLDVSIPSATFAAAGPYYL
                     IVTNSYGSATSSVAQVTVGAVASGLFVTGIGTNGQVSSGDVPDPHYTVIQSSDPSYLG
                     PTTLIFEWNYPIEFSPNSGGYAPTNGDSTWIGLQGNQGGVINNSPAGNYTYRTTFLLD
                     QANPSTVTLQGSVVFSGLITNILINGKSTGISLAPVNGLYIASPFTITNGFVPGINTL
                     DFCQQLASAQNTAIYVAQVSAIGQAVAPGLPVILQQPTNETVRDANLTGPGSIAEFSV
                     SATGCPPLTYQWWADGVPLAGETNYTLSIYNPSAGAQGTNYYAVVTNSSGSVTSSSAV
                     LTLVSTNQPPVCQTLNLVAFNNENVNVQISYLLDVLSSDPDHDNISYDSSDTASTNGS
                     ANGLNNVNQSGATLVYTPVAGFVGPDQFTYTIQDSLGTMTSGNVNVLDLSSPSPFTTV
                     PVGGTTNLNAGVSNVPPGYSFQWQLYGANLPGTTSAQLTFSNAQSALDGAYQLLVGDQ
                     AGNVVSSSLARVAVNGAPRPGFGVNPMDWVFNGYGTGSTLTNNVLTLTDGTLSEGRSA
                     WYGWPQVITNFSASFVYRDTGGGGADGFTFVLQNSPAGTAALGSGGGGLGYAGIANSV
                     ALEFNIYAPNTVGIALQSGGAVSPPFTPTTPLNIASGDPIQVNISYSAGVAQLALTDL
                     TTLESTNFTLDATSVLGDTFENILGTNIAYVGFTGGDGGVASTQTISNLLFIPPSLSL
                     SFQQTASNKAVLSWTPPYNGFLLQTSGNLGGSWQNVSVPINTVGGQYQVQVPMSGGSE
                     FYRLMLP"
     gene            complement(6663..6908)
                     /locus_tag="VG754_09365"
     CDS             complement(6663..6908)
                     /locus_tag="VG754_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19467.1"
                     /translation="MFDGGSYVRRHPLFDHSQRHQRVQEVLLALFHMRRQLMLVTNEN
                     PAPAATGANNVRLVADHMALLPRHYSMIAFVYGNISL"
     gene            7163..7768
                     /locus_tag="VG754_09370"
     CDS             7163..7768
                     /locus_tag="VG754_09370"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWF19468.1"
                     /translation="MVNLKNRATRCEVFILAGGLSARMGRDKSRVRLGPQTMLEHVRA
                     AAARTGLPVRVIRRDAVPRCGPLGGIYTALKRSRAEMVLFLACDMPFVPSELLERMVN
                     AFGRDGALRSTLRKDGAARRPCQFATALFASKNGRAGFPFLLRREFALPVVTKQIQAG
                     EYSLQSLAKKLRAKTLRFSAGQLANINTPAELKKAERLFRS"
     gene            7874..>8737
                     /locus_tag="VG754_09375"
     CDS             7874..>8737
                     /locus_tag="VG754_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWF19469.1"
                     /translation="MLKKTLICSAICVCAAHGVFSDTIQLADKAALTGKILAEKHDQV
                     VMDVGYTVLVIPRDSIVKISKSGDSQAATVVASLTPMIPLSGSSSALVDSKNGGYQTA
                     STPPPVKDVRELVNQLGESVVQVRTPGGLGSGFIINEDGYLITNFHVIEGETEISVEV
                     YHMKDGQLERKSYKDVKIIAMNKFEDITLLKIDDKDAPKFSRVLLGDSDSLAVGEKVF
                     AIGSPLGLERTVTEGILSTKTREMQGELYLQTTAQINPGNSGGPLFNTRGEVVGITNM
                     KITFGEGLGFAI"
BASE COUNT         1961 a         2499 c         2347 g         1930 t
ORIGIN      
        1 gcgccttcat tacacgaccg attggttcgt cgataacgaa caaaagggcg ttgtcaaaat
       61 tttggcgccg gaactgcccg gcgcggagcc gttcacgcaa aaagtcggca tcatgtcgca
      121 acaccccgag cattaccggc agctcaaatc ccatcccgaa ctcattcccg ccatcaaggc
      181 ggccgaggac agaatcaacg cgttttcatt gaaatatgtg ccgatggaca aactgaaagc
      241 cgcggaaccg ttgcttgtga caggcgacat cgtgggcgtg gccacgaccg aagaaggcat
      301 tgacatcgcg cacaccggcc tctgcatcaa ggatgagcaa ggcgtggtgc atttcatgga
      361 cgcttcatca tcgcgcagaa acatgaaagt caccctcgaa cgcgagatca gcgcctgcct
      421 gaactggtcg cccaaattga ccggggcgat gttcgcgcgg cccttggaag tcgggaaagt
      481 ttgagcgaat cttagtttta ggcccggaac gggccggacg tgaatggccg caggtgaaat
      541 ctgcgggtag cagcaaggag aattgtccgg ccctgtcagg accactggtc tgttggcgta
      601 tccgtgggtt tacacccatt tgcccgctat ctggtttggg catggctgtt tgcacagcca
      661 tctggaggat ttggacgacg accgccaatt ctttttcatg gttcgctggt cttggggttt
      721 tttcttgctg caacccggtt tgattaccac tcttcttcca tgaacttcca gaccgcgaat
      781 tgcggatttt gccttcggcc ggagctccgg cacgaacgat gcaactggaa gcgccagcca
      841 cgaaagttca taaacattct ttccataata atcctgtcag tttttgcggc cggcagttac
      901 acgatcgcat ttgcaaatga aacagacaaa cgcggcgcca gcccgatcac gtcggttggt
      961 gaagcaacca atcattgcgg ctcgtggata tgggacaagg tgacgagtga caagcagacg
     1021 tgtcactttt ggaggcggtt tgaaatcccg gcaggaggcg aagtgttgca cgcacaactc
     1081 cgcgtcacgg cggacaatgc ctatacggtc tatctcgacg gccgtgagct tggacgcggc
     1141 agtgattggc gcactctaag cgcttatgat ttgagctggc ttttgcaacc gggccctcat
     1201 gttctggctg tggatgcgtt taacgacgtc aacaaggccg gagtcattgc aggcttgcat
     1261 gccgaacttt ccgacggtcg aaccatcgaa atcaagtccg acacaaattg gcttgttgtt
     1321 ccgttgaccg atcttgattg gctgacgaaa cgcaaggcat cccgcgactg gccgcatgcc
     1381 attgtggtcg ggctgttggg ggagggccca tggtggcaac cgacgccaac cgaaatcgcc
     1441 aacatagcgc cattatttcc gctcaagacc catttctggg aaaatggctg gtttcaaatc
     1501 acccttctgt cattttgtgt tttgaccttg atcacctccg cacggctgat gacccagctt
     1561 gcggtccaag cccgcgccgg ccggttgttg caatcggaac gcgcccgcat cgcccgggac
     1621 atccacgacg atcttggagc acggttgacg aagttattgg tgaccggcga agaagcgcaa
     1681 gaccggttat tggagcaggc ggatgcccgc acaaatgttg atcaaatatg cgatggcgcc
     1741 cgcgacatcc tgaatgccat tgacgaaatc gtctgggtcg tcaacccgca acgggacaag
     1801 ctgaatgact ctctcatcta catctgcaag tatgcggaat cgtttctccg cgctgcttca
     1861 gtgcgttgca ggtttgatat tcagtccgac atgccggaaa tctcgctgga cctcccgttt
     1921 cgccgaaata tttttttgac gatcaaggaa agtctcagca acgcggtcaa acattccggc
     1981 gcaacggagg tttcgatcca ggtccggctg gacgggccca cgctaagagc gttggtggag
     2041 gacaacgggc atggatttga tccatctcaa atcaacacga cgcggcacgg tttgagcaac
     2101 atgattgctc gaatcaagga tttgggtgga aattgccggg tggaaagcaa gccggggacc
     2161 ggctgccggg tggctttcag tgttcccctg ccgctggccc gctcgcaaca atgggtggta
     2221 aaatggtggt acggcctttc ctccagatga aacatcgcag gcaccgcaaa tggattaccg
     2281 tttcgcaggc gtccccgttt gaggacatgg acattgattg cgcgagtttc ttaaatcgaa
     2341 gcggtggtat gggtgattat ttgccatgaa gcaacaacct gcaattgagc cagtcaagtc
     2401 aacccgcaaa tcgccttttc gcgtggccgt ggtcgaggac gagcagttgt tccgggaaag
     2461 ttgggcgcgc cgcatcaact cctttccgga tttcaaatgt ctttgcgaat gcgcttccgg
     2521 cgaagaagcc ctcgccatgg tgcctggcaa gcagccggac gtaatcctga tggacgtttt
     2581 gctgcctgga atttccggga tcgagtgcac cttccggctc aagaagctgt tgcctgaatc
     2641 cctcattgtc attctaacgg ccttggatga cgatgaactc atttttcgcg ccttggaagc
     2701 gggggccgac ggctatttgc tcaagcgcac caagcctgtt gacgtgcgcg ccgctctgct
     2761 ggatgtcctc agcggcggcg ctccgatgac gagcgccatt gcgcgccggg tggtaaagtt
     2821 ttttcggaag gagccggttg caaagcagga atcaatccgc ctttccgcgc gggaggagga
     2881 aatgctggag cttctttcaa aaggttattc aaacaaggaa atcgccgaac agagccatct
     2941 gacacttgag acagtgcgaa cttacctgaa acgaatctac gaaaaaatgc acgtccgttc
     3001 ccgcacggaa gccgcagttc gttacgtcca tttcaaatct ccttgagctt ccgagccaac
     3061 ctggatgtcc ccgattgggg acatggacgg caaagctcac ctggcctaaa gtttttttca
     3121 atcacagccc ggttccaatt gcaatggcgc ttgtcaaggc cggcaccggg cggcgtcatg
     3181 tcaatcacaa gtggaaaact gaccgattca ttcaagcgaa atatgaaaaa aaccactctc
     3241 cttccatcca accccttgct ttggggcatc gctctggcgc tggtcttatt ctattcccag
     3301 acagccaggg ccggtcttgg cattccctac tcgcaggatg cggacacaat gcacctctgg
     3361 catttgaatg atgcaaccaa tcagttatat gcggttgatt ccgccaccaa cccggttgat
     3421 ggcgccattc cgatccagtt ggaaaatatt ggcgaaccga ccccgggcca acagcccacc
     3481 aacgcattgt acggcagtcc aggcccgggc tatccgggac tgggcagttg ctattccggc
     3541 agcacgaaac aacatgtttt ggctggatat aatgggtcct cgtatggcgg cgacttcccg
     3601 gacgtttccc aattctgcaa tccgaacaca ggagcgttca cctttgaagc gctgatcaat
     3661 ttgggcagcg gaagttctcc gctatcatca gtggacgctg aaatcctttc cggtgacaat
     3721 aacggcggcc ttggtaatcg cggatggcaa tggcgcattg agggcgggac catggagtgg
     3781 gacctcctcg ctggcagcac ggacaacgat ttcaaggcac ccctcccgac ctcgggccca
     3841 aacgcggtca aactgaatgc ctggtttcat gtggctgtga cgttcaccgg ccaaagcccg
     3901 accaatggag atcccgccaa tgtgtttacc atgtactgga cggcgctgga tcccagccga
     3961 acgtcggccg atgtcctggc gcaatacacg aacgacgttg cttctccata cggcatccgg
     4021 ccgttgagcg gtgcgcccga agggactgcc acacctgcct tgggcatcag cggcagttct
     4081 cggaatactt cctccaatcc aggcaacaac gaaggcatga tcggcagcat catggagctt
     4141 cgggtcagcg acatcgcccg ccaatcaaac cagatggcct ttgtcgcggg cggatcattt
     4201 ccaccggcga tcacccagca gccgccaaca aatactttgg tgggatacgg ccaaccgctc
     4261 gtcttcggtc ctacggcgtc cggcacggcg cctctgcagt acgagtggtt tcagggcggc
     4321 acgaatttgg cttcccaaac caatcttgac gtgtcgatcc caagtgcaac ctttgccgcc
     4381 gcaggcccgt attatctgat agtgacgaac tcttatggga gcgcgaccag ttcagttgcc
     4441 caggtcacgg ttggcgctgt agcatccggc ctctttgtca caggcatcgg cacaaacggg
     4501 caagtttcct cgggagacgt tcccgacccg cattacacag tgattcaaag ctcggacccg
     4561 agttacctcg ggccaaccac cttgattttt gaatggaatt atcccattga attttcaccc
     4621 aacagcggcg gctacgcccc gaccaatggc gattcgacgt ggattggcct tcaaggcaac
     4681 cagggtggcg tcatcaacaa ctcccctgcc gggaattata cttaccggac aactttcttg
     4741 ctcgatcaag ccaatccatc cactgtgaca cttcagggct cggtcgtttt cagcggcctt
     4801 atcactaata ttttgatcaa tggcaaaagc acggggatct ccctcgcgcc ggtaaatggg
     4861 ttgtatattg ccagtccatt caccatcacc aacggctttg tgccgggcat caacacgctt
     4921 gatttttgcc agcaactcgc cagcgcccag aacaccgcaa tttacgtggc ccaggtctcc
     4981 gcgatcgggc aagccgttgc gcccggcttg cccgtgattt tgcaacaacc caccaatgaa
     5041 accgtgcggg atgccaacct cacgggtccc ggcagcattg cggaattctc cgtgagcgcc
     5101 acgggctgtc cgccattgac ttatcaatgg tgggctgatg gggttccact tgccggagaa
     5161 accaactata cgttgtcaat ttataatccc agcgcgggag cccaagggac caactattac
     5221 gctgtcgtca cgaacagctc cggttccgtc accagctctt cagccgtgct gacgcttgtt
     5281 tcgaccaacc aacctcccgt ttgccagaca ctcaacctcg tggcttttaa caacgaaaat
     5341 gtgaatgtgc aaatctcata cttgctcgac gtgttgagca gcgatccgga ccacgacaat
     5401 atttcatatg attcatcgga caccgccagc accaacggtt ccgccaacgg tttgaataac
     5461 gtcaatcaaa gcggcgccac gctggtgtac acgccggtcg caggttttgt cggccccgat
     5521 caattcacct acacaatcca ggattcgctg ggcacgatga cttccggcaa tgtcaatgtc
     5581 ctggatttgt cttcgccttc gccatttaca accgtgccgg tgggcgggac cacgaacttg
     5641 aatgcgggtg tcagcaacgt gccgccggga tattcgttcc aatggcagct ttacggcgcc
     5701 aatttgccgg gaactacatc cgctcaactc acattttcaa acgctcaatc tgctctggac
     5761 ggcgcgtatc agttgctcgt tggtgaccaa gcaggaaacg ttgtttccag ctccctcgct
     5821 cgcgtggctg tcaatggcgc accaagaccg ggcttcggcg tcaatccgat ggattgggtg
     5881 ttcaacggtt acggcacagg cagtacgctc accaataacg tgctgacttt gacagacggc
     5941 acactgagtg aagggcggag cgcgtggtat ggctggccgc aggtcatcac caacttcagc
     6001 gcctcttttg tgtatcgcga caccggcgga ggcggcgcgg acggattcac atttgttttg
     6061 caaaattccc cggccggcac agcggcgttg ggcagcggcg gaggcggctt gggttatgcg
     6121 ggaattgcta atagcgttgc tttggagttc aatatttacg cccctaacac tgtcggaatt
     6181 gccctgcaat ccgggggcgc cgtatcgcct cctttcactc ctacaacccc gctgaacatc
     6241 gccagcggcg atcccattca ggtgaacata agctactcgg cgggcgtggc ccaactcgcc
     6301 ttgaccgact tgacgaccct ggaatcaacc aacttcacct tggacgcgac aagtgttctc
     6361 ggcgacacct tcgagaacat cctcggcaca aacatagcct atgtcggttt caccggcggc
     6421 gacggcgggg tcgcctccac tcaaacaatc agcaaccttt tattcattcc tcccagcctg
     6481 tcactttcgt ttcaacaaac cgcgtcaaat aaagcggtac tttcgtggac gcccccatat
     6541 aatggttttc ttctgcaaac gagcggcaac ctcggcggat catggcagaa cgtctctgtg
     6601 cccatcaaca ccgtcggtgg acaataccaa gtacaggtgc ccatgtcagg aggatctgaa
     6661 ttttacaggc tgatgttgcc gtaaacaaaa gcgatcatgg aatagtgtct tggcaataac
     6721 gccatgtggt ctgcgaccag ccttacgtta ttggcgccgg tcgcagcagg cgctggattc
     6781 tcgttggtga cgagcattaa ttgcctccgc atgtggaaca gggcgaggag cacttcctgc
     6841 acgcgctggt gtcgttgcga atggtcgaac aaggggtggc gccggacata agaaccgccg
     6901 tcgaacaact ggaaaattgg tgggaggaag atgaagagtt acgcctcgcg aatggccggt
     6961 tgaatccagt agaacgcgta ggttgaaaac cggctggcct cgatcccacc aagctggaat
     7021 cacattgcac taacggtaaa gacgaatata caatctttta taaaaatgat ctgaactgaa
     7081 cgaagtgctt ataacgcgat ccattgtttc aggtgtattg tttctcgttt aaaaccaaaa
     7141 tcagctagac aggattggaa gcttggtaaa ccttaaaaac cgcgccaccc gttgcgaggt
     7201 attcatcctc gcgggcggtt tgagcgctcg catgggtcgc gataaatccc gcgtgcggct
     7261 cggcccgcaa acaatgctgg aacacgtccg cgccgcagcc gcacggaccg gattgcccgt
     7321 ccgcgtgatt cgccgcgatg ccgtgccgcg ttgcgggccg ctcggcggga tttataccgc
     7381 gcttaaacgc agccgcgccg aaatggtgct gtttctcgct tgcgacatgc ctttcgtgcc
     7441 gtcggaattg ctcgaaagga tggtgaatgc ctttggcagg gacggcgcgc tgcgatctac
     7501 gctcaggaag gatggcgcag cgcgccgtcc ctgccaattt gccacggccc tcttcgccag
     7561 caaaaatggc cgcgctggtt ttcctttcct cctccgacgt gaattcgcgc tccctgtcgt
     7621 taccaaacaa atccaagccg gcgagtattc cctgcaatct ctcgccaaaa aacttcgcgc
     7681 gaaaacgctc cgtttttccg ccgggcaact ggccaatatc aatacgcccg ccgaactcaa
     7741 aaaagccgaa cggctgttcc gtagctgaaa acgtgagttt tcagccattc atggcccgca
     7801 tgttccacaa ataccgacaa agtttattca agctcagctt gtcaaacgtg tgcgcaaccg
     7861 ctaatatcag cacatgttaa aaaagacttt gatttgctcc gcgatatgcg tttgcgcggc
     7921 ccacggcgtc ttctccgata ccatccaact cgcggacaaa gccgcgttga cgggcaaaat
     7981 cctcgccgaa aagcatgacc aggtcgtcat ggacgtcggc tacaccgtgc tcgtcatccc
     8041 gcgcgattcg attgtgaaga tttccaagag cggtgattcc caggcggcaa cggtcgtcgc
     8101 ctcgctcacg cccatgattc ccctgagtgg gtcgtcgtcg gcattggttg attcgaaaaa
     8161 cggcggttat caaacagcca gcacgccgcc tcctgtgaag gacgttcgcg aactcgtcaa
     8221 tcaattgggt gaatcagtcg tgcaagtccg cacgccgggc ggtctcggtt ccggtttcat
     8281 catcaatgaa gacggctatc tcatcaccaa tttccacgtc atcgagggcg aaaccgaaat
     8341 ttccgtcgaa gtttatcaca tgaaggacgg ccagttggag cgcaagagct acaaggacgt
     8401 caagatcatc gcgatgaata aattcgagga catcacgctg ctcaagattg acgacaagga
     8461 cgcgccgaaa ttttcgcgcg tgctgctcgg tgattccgac agccttgcgg ttggcgaaaa
     8521 agtttttgcc atcggcagtc cgctcggctt ggaacgcacc gtgaccgaag gcatcctgag
     8581 caccaagacg cgcgagatgc agggcgagct ttatctgcaa accaccgcgc agatcaatcc
     8641 cggcaacagc ggcgggccgc tcttcaacac gcgcggcgaa gtcgtcggca tcaccaacat
     8701 gaagatcacc ttcggcgaag gtcttggttt tgccatt
//
LOCUS       DASZMM010000385         6945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1802941, whole genome shotgun sequence.
ACCESSION   DASZMM010000385 DASZMM010000000
VERSION     DASZMM010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802941"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VG754_09380"
     CDS             complement(<1..521)
                     /locus_tag="VG754_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HWF19470.1"
                     /translation="MSIEHRTLDTQEDFLEATTDTLMPERIESSDTDRIGRCRRSGHL
                     PLSFAQQRLWFLDQLHPESSLYNVPVALRLEGALNECALRRSLATIVERHGVLRTRFV
                     AMDGNPTQFISPNAAVELPVKDLSGLPEASRQAEAHRLLAAEAKQPFNLAQDLMMRCL
                     LLRMAAHENILMVT"
     gene            915..2435
                     /locus_tag="VG754_09385"
     CDS             915..2435
                     /locus_tag="VG754_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19471.1"
                     /translation="MVAPRAADARHAEKALRSEGLKLSLKLASSPRAFTDALQKEPPD
                     LVIFDLLTPKFSALKAVQLAHAVQPHLPFILLASPRDKTKISRELRQKMTDLVSKGRA
                     ADLAAAVRRALREMHGQKQRARAEAASRHSEELLRLLIEQVTDYAIYVLDAEGRVASW
                     NVGAERMTGYSFEEAMGRPLNHCFNRVEIKEGKPARELQTALAKGRAQSEGWCRRKNG
                     TTYYAQWTITAIRDASGKPAGFLKVARDVTERQQHADAVAKMNADLEQRVRERTAQLE
                     DANQELEAFSYSVSHDLRAPLRHIDGFIEILQENVAAKLDAESREHLQTIAQAARQMG
                     KLIDALLAFSRLARTRVNKRKLNLDVLLGDVLAHVKNETQGRKIQWEVDKLPIVEADP
                     ILLKQALLNLVSNAVKYTRTRPRARIEIGTTVNRREHIIFIRDNGVGFDMQHIDKLFG
                     AFQRLHRANEFEGTGVGLANVRRIIHRHGGRTWAEGAVNKGATFYLSLPKQVKENQ"
     gene            2582..3751
                     /locus_tag="VG754_09390"
     CDS             2582..3751
                     /locus_tag="VG754_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19472.1"
                     /translation="MRKPIRILMVEDSDADATLVRHALRKDGLSFTMDRVETAEDYRH
                     ALENAPPDVILSDYALPSFDGYMALAIARERQPYVPFIFVTGTMGEEVAIETLKNGAT
                     DYVLKVRLSRLAPAVRRAMREARERADRKRAEEQLRRSHEQLHALSMYLQHVREEERI
                     RISRAVHDELGQALTGLKIDLSWLGSRLPKNLEPLIHKARKMSVHIDETIKTVRRIST
                     ELRPGILDHLGLAAALEWQANEFQTRSGIVCKVASTLKRTILDEEINTVFFRIFQETL
                     TNIIRHANATRVEVRLSEEHRPNQLMLEVNDNGRGITSEQISSVRSIGLLGMHERAAL
                     LGGELTIRGVPKHGTHVTVTIPFSPPVRSNKRHNENTDSRRSRSSAARLETNSRR"
     gene            3684..4313
                     /locus_tag="VG754_09395"
     CDS             3684..4313
                     /locus_tag="VG754_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF19473.1"
                     /translation="MKILIADDHAVVRHGLKQILADEFKKSVFGEAGNAQEALEKVWK
                     EPWDVVVLDITMPGRSGLEVLREIKKYSPKLPVLVLSMHPENQFAVRVLKRGASGYMT
                     KETAPAELVGAIKKVLAGGRYVSAALAEKLATNLSTDTQKPPQELLSDREFQVLRLIA
                     SGKIVSEIARELSLSVKTISTYRTRILEKMGMKNNAELMHYAMQHELVE"
     gene            complement(4317..5777)
                     /locus_tag="VG754_09400"
     CDS             complement(4317..5777)
                     /locus_tag="VG754_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWF19474.1"
                     /translation="MTNTPFDLKARARQAMFDAGFHPDFPPEVLREAQNLKQTSPQIS
                     NPPPRDMRELLWSSIDNDSSRDLDQVEYVEKLANGTTRLLVGIADVDASAPKNSATDK
                     RAAMETTSVYTGVTNFPMLPNEFSTDATSLLGDQDRVSLVIELHVKDSGEVACHDVYP
                     ALLRNRAKLAYNSTGAWLEGRGPMPEPIARVQGMEAQLRLQAEISQKLRGIRKQRGSL
                     TFGQIEAMPVVENGEVKDLAIVRHNQAEYIIESFMVAANVAMAQHLKEEGVVSIRRVV
                     RTPKRWDRIQSLAQQFGVKLPDVPDPRSLSDFLDQRKAADPDHFPDLSLSVVKLLGPG
                     EYIVERPGAEPNGHFGLAVEDYTHSTAPNRRFADLVTQRLLKATIAHSSLPYSEGELA
                     DIAKRCTEREDAARKVERLMRKVIAANLLSRQIGQMFDGIITGATSKGTFVRLLKFPA
                     EGMLVRGAKGVDVGDKVRVKLVGTNAAMGYVDFDRL"
     gene            complement(5879..6673)
                     /locus_tag="VG754_09405"
     CDS             complement(5879..6673)
                     /locus_tag="VG754_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWF19475.1"
                     /translation="MKCIALKGFILLLLFTAGFLVSWHHFYILSLHESPMQSIHTTLN
                     EVPAWQGYWLYTELRHPLYAWIVFLLTFGGTFLTKRGLFLLAPVCVTVQGVSFGAFEY
                     VLQTRYPGTTFFCITVVVGLFLGVLLVYWFLSAQTDNPLTLYAVGVSVGILFSYLISA
                     WGRSLGVKVPFLHELPMNWGGGALVFYAVGCGILLNTLVNIKEAIETGAPKWAEWRAA
                     MILFGYFLMLLKLCRFILLRRLQGQLTWERLNDAFKVISQAGSNDR"
     gene            complement(6716..>6945)
                     /locus_tag="VG754_09410"
     CDS             complement(6716..>6945)
                     /locus_tag="VG754_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045243001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWF19476.1"
                     /translation="SAVVGANLLPRANRVAALLQRWLLGTHQGAVKMDHLDYYLDEFT
                     FRSNRRTSGSRGLLFQRLVEQGVSARPGARR"
BASE COUNT         1647 a         1984 c         1857 g         1457 t
ORIGIN      
        1 gtcaccatca aaatattttc gtgcgccgcc attcgcagca gcaggcaacg catcatgagg
       61 tcttgggcca gattgaaagg ctgcttcgct tcagcggcca gcaatcggtg tgcttcggcc
      121 tggcgcgatg cctcgggcag cccgctcaag tcttttaccg gcagttcaac cgccgcgttt
      181 ggcgagatga actgcgttgg attgccgtcc atcgcgacga accgtgtccg caacactcca
      241 tgccgttcga cgattgtggc caaagaacgc cgtaacgcgc attcgttgag cgctccctca
      301 agcctgagtg ccacgggcac attgtagagc gaactttcgg gatgcaactg gtccagaaac
      361 cagagccgtt gctgggcaaa agaaagcggc aaatggcccg accggcggca acgtccaata
      421 cgatctgtgt cggagctttc aatccgctcc ggcatcagtg tgtcggttgt tgcttccaga
      481 aaatcctctt gtgtatccag tgttctatgt tcgatggaca tggtggtgac atggtcagct
      541 caatcaacgc cacatcgtaa ggtgatgaca tgaaagcagt aagtaggtga aaaccgccca
      601 atgcacggca gaagacccca cgcccaatcg aagcttgcca tgcgcgacaa gcaactcgcg
      661 catgtttctt gtccacggat caacactgat tcagaaaaac gaaatgaata tgttcaaaaa
      721 cttttgtgtt tggctatggc ggcccttgtc ggagtccaaa aagttataac gtgattccat
      781 gcatgtctgt gtcattcgcg ctgtgtatga ctatttttta ggtcatcacc ccattggagg
      841 ggagcgattg cgacattact gttgcgcgtt ggaattccaa gctgttcaga gtgaagaccg
      901 gtaattctat tttgatggtt gcgccgcgcg cagcggatgc ccgccacgcc gaaaaagctt
      961 tgcgctctga agggctgaaa ttgtcactga aactcgcctc ctcaccacgc gcttttaccg
     1021 atgcgcttca aaaagagccg cctgacctgg tgattttcga tttgttaacg ccaaaattta
     1081 gtgccttgaa agcggtgcaa cttgctcacg ctgttcaacc gcatcttccg ttcattctcc
     1141 tggccagtcc gcgggataaa acaaaaattt cgcgcgaact gaggcagaaa atgaccgatt
     1201 tggtgtccaa aggacgcgct gccgatcttg cagcagcggt gcgccgggct ctgcgcgaaa
     1261 tgcacggaca aaaacagcgc gcgcgcgctg aagctgcctc acggcacagc gaggagttgt
     1321 tgcgcctgct catcgagcag gtgacggatt atgcgattta tgtgctcgat gccgaaggac
     1381 gggtggcaag ctggaacgtc ggcgccgagc ggatgaccgg gtattccttt gaggaagcaa
     1441 tgggacgccc gctaaaccat tgtttcaatc gcgttgaaat caaggagggc aaaccggccc
     1501 gcgaattgca gaccgcgctc gccaagggcc gagcccaatc tgaaggatgg tgccgacgca
     1561 agaatggcac gacctattat gcgcaatgga cgatcactgc catccgggat gcatcgggca
     1621 agccggcagg ttttttgaaa gtggctcgcg acgtcacaga acgtcagcaa cacgccgacg
     1681 cggtcgcaaa aatgaacgcc gatctggagc aacgcgtacg cgagcgcacc gcacaactgg
     1741 aagatgccaa ccaggaactt gaagctttca gttactccgt ctcacacgac cttcgagcgc
     1801 cattgcgtca cattgacggt ttcatcgaaa tcttgcagga aaatgtcgcg gcaaagctcg
     1861 atgccgaaag ccgcgaacac ctccagacca tcgctcaggc ggcgcgacag atgggcaagc
     1921 tcattgacgc gttgctcgcc ttctcgcgcc tggcccgcac ccgcgtcaac aaacgaaagc
     1981 ttaatttgga cgtattgctg ggcgacgtcc ttgcccatgt gaaaaacgaa acccaaggcc
     2041 gcaagattca atgggaggtc gataaattac ctatagtcga ggccgacccc attcttttaa
     2101 aacaggcgct gctcaatctg gtttccaatg ccgtcaaata cacacgcacg cggcctcgtg
     2161 cccgcattga aatcgggacc acggtcaacc gccgggagca catcattttc atccgcgata
     2221 acggtgtcgg ttttgacatg caacacatag acaagttgtt tggcgccttt caacggttgc
     2281 atcgcgcgaa tgagtttgaa ggcaccggcg tcggcctcgc caatgtgcgc cggatcatcc
     2341 atcgccacgg cggccgcacc tgggctgaag gcgctgtgaa caaaggggca actttttatt
     2401 tgtcgctgcc caaacaagtg aaggaaaacc aatgaatcgc ttgccaaaaa atacccgtac
     2461 ggctaatctt gcagcatcca gccggcattt ggcttgtccg gttggtaatc agaagcagtt
     2521 ttcgccctgt tccaacttca cgattaattt cggttgtttc ggccagccat gccgttagca
     2581 catgagaaaa ccgatccgaa ttttgatggt tgaagacagt gacgcggacg cgacactggt
     2641 ccgccatgct ttgcgcaagg acggcctctc cttcaccatg gaccgggttg aaaccgccga
     2701 ggattaccgt cacgccctcg aaaacgcccc gcctgacgtc atcttgtccg attatgcgct
     2761 cccatccttc gatggttaca tggccttggc catcgcccgc gagcggcagc cttatgtccc
     2821 gttcattttt gtgaccggaa ccatgggcga ggaagtcgcc attgaaactc ttaagaacgg
     2881 cgcgacggat tatgtgctca aagttcggct ttcacggctc gctcccgccg tccgacgcgc
     2941 aatgcgcgaa gcccgcgaac gcgctgaccg aaagcgcgcc gaggaacaat tgcgccgctc
     3001 gcatgaacag ctgcacgcgc tttccatgta tttgcagcat gtccgtgagg aggagcgcat
     3061 ccgcatctcg cgcgcagtcc atgacgaact tggccaggca ttgaccgggt tgaagattga
     3121 cctttcgtgg ctgggcagcc gtctgccgaa gaatttggag ccgttgattc acaaggcccg
     3181 gaaaatgtcg gtgcatattg atgaaaccat caagaccgtg cgccggattt ccactgaatt
     3241 gcgtcccggc attctcgatc accttggcct tgccgccgcc cttgaatggc aggcgaatga
     3301 atttcaaacc cgctcgggca tcgtctgcaa ggtggcgagc actctgaagc gcacgattct
     3361 cgacgaagag atcaacaccg ttttctttcg catttttcag gaaacactga ccaacatcat
     3421 tcgccacgcc aacgccacgc gcgtggaggt gcgcctgtcc gaagaacatc gtcccaacca
     3481 gctcatgttg gaggtgaatg acaacggccg cggcatcaca tccgagcaga tctccagtgt
     3541 ccgctccatc ggcctgctgg ggatgcacga acgcgcggcg ttgctcggcg gcgagctcac
     3601 catccggggc gttcccaaac atggcactca cgtcacggtg acgattccgt tcagccctcc
     3661 agtacgatcc aataaacgtc acaatgaaaa tactgatagc agacgatcac gcagtagtgc
     3721 ggcacggctt gaaacaaatt ctcgccgatg aatttaaaaa atcggtcttc ggcgaggccg
     3781 gcaatgccca ggaagcactg gaaaaagtgt ggaaagaacc ctgggatgtc gtggtgctgg
     3841 acatcaccat gcccggccgc agcggcctgg aagttttgcg cgagataaaa aaatattcgc
     3901 ccaaactccc cgtgctggtc ctgagcatgc atcctgaaaa ccagttcgcc gtgcgcgtcc
     3961 tcaaacgcgg cgcttccggt tacatgacaa aggaaaccgc cccggccgaa ctcgtgggcg
     4021 ccatcaagaa ggtcctggcc ggaggccggt acgtcagcgc cgcgctcgcc gaaaaacttg
     4081 ccaccaatct ctcgacggac actcaaaaac caccgcagga actgctttca gaccgcgaat
     4141 ttcaggtgtt gcgcctgatc gcctcgggaa aaatcgtcag cgaaatcgcg cgggagcttt
     4201 cgttgagcgt gaaaaccatc agcacctatc ggacgcgcat cctcgaaaaa atggggatga
     4261 aaaacaacgc cgagctgatg cactacgcca tgcagcacga gttggtggaa tgatatctag
     4321 agccggtcaa aatccacgta ccccatggcc gcgtttgtcc cgaccaattt gacccgcacc
     4381 ttgtctccca cgtccacgcc ctttgcgccg cggaccaaca tgccttccgc cggaaatttc
     4441 aataaacgca caaatgttcc cttcgacgtt gcgcctgtga tgatgccgtc aaacatttgg
     4501 ccgatttgac gactcagcaa atttgctgcg atgacctttc gcatgagtcg ctcgactttt
     4561 cgcgccgcat cttcgcgttc ggtgcagcgt tttgcgatgt ccgccagttc tccttccgaa
     4621 taagggaggg atgagtgtgc gatcgtggcc ttgagaaggc gttgcgtgac caaatccgca
     4681 aaacgacggt tgggcgcggt ggaatgagtg taatcctcaa ctgccaggcc gaaatgtccg
     4741 ttcggttccg cgccgggacg ttcgacgatg tattcgcccg ggccaagcag cttcaccacc
     4801 gaaagcgaca gatcaggaaa atgatccggg tcggccgcct tgcgctggtc cagaaaatca
     4861 gaaagtgagc gcggatcggg aacatcggga agtttcaccc caaattgctg cgcaagggat
     4921 tggatgcggt cccaacgctt gggcgtcctc accacgcgcc ggatggaaac cacgccttct
     4981 tccttgagat gctgcgccat tgcaacattt gcggcgacca tgaaactttc aatgatatat
     5041 tcggcctggt tgtgacggac aatcgccagg tctttcacct cgccattttc aacgaccggc
     5101 atggcttcga tttgaccaaa cgtgagcgaa ccgcgttgtt tgcggatgcc gcgcagtttt
     5161 tgcgaaattt ctgcctgaag ccgcaattgc gcttccatgc cctgcacgcg agcaatgggt
     5221 tccggcatcg ggccgcgtcc ttcaagccat gcgccggttg aattgtaagc gagcttcgcg
     5281 cgatttcgca gcagggctgg ataaacatca tggcaggcca cctcgcctga atccttgaca
     5341 tgcaattcaa tgaccagcga tacacggtcc tgatcgccca gcaacgaagt cgcgtcggtg
     5401 gaaaattcgt tgggcagcat cggaaaattc gtcacaccgg tatagaccga tgtcgtttcc
     5461 attgcggcgc gtttgtcagt ggcggaattt tttggcgccg aagcatccac gtccgcgatg
     5521 ccgaccagca gccgcgttgt gccattggca agtttttcaa catattcgac ctggtccaag
     5581 tctcgcgagg aatcattgtc gatcgaagac caaagcagct ctcgcatgtc cctgggtgga
     5641 ggattggaga tttgcggcga cgtctgtttg aggttttgcg cctcgcgaag aacttcgggc
     5701 ggaaagtccg gatgaaatcc ggcatcgaac atggcttgcc gcgctcgggc tttcaggtca
     5761 aatggcgtgt tggtcatccc gtatgatggt tttggctggg ggcacccgca agttttttac
     5821 gccggacgga gacgaaacgc gaagttttca cggtttccac ttgctgcaac gatcgcactc
     5881 agcggtcatt ggatccggcc tgtgatatta ccttgaacgc gtcattgagt cgctcccagg
     5941 tcaactggcc ttgcaaacga cgcagcagaa taaaccggca cagcttgaga agcatgagaa
     6001 aatagccaaa caggatcatg gcggcgcgcc actccgccca ctttggcgcg ccggtttcga
     6061 tggcctcctt tatattaacg agagtattca gcagaatgcc gcaaccaacg gcatagaaca
     6121 ccagtgcgcc accgccccaa ttcattggca gctcatggag aaatggcacc ttgacaccca
     6181 agcttctgcc ccacgcgctt atcaaatagg aaaaaagaat tccgacgcta acgccgactg
     6241 cataaagcgt gagcggattg tctgtctgtg cggataaaaa ccagtaaacc agaagcacgc
     6301 ctagaaagag tccgacaact actgttatgc agaaaaaagt ggttcccgga tagcgggtct
     6361 gcaacacata ttcaaaggcc ccaaaactta ccccttggac cgtcacacag accggcgcca
     6421 gcaagaacaa gcctctcttg gtcaggaaag taccgccaaa cgtcagaagg aacacaatcc
     6481 aagcgtatag gggatgccgc aattcggtat ataaccaata accctgccat gcaggcactt
     6541 cgttcaaggt ggtgtgaatg ctctgcattg gcgattcgtg caaagagaga atgtaaaaat
     6601 gatgccagct cacgagaaat ccggcagtga acagcaacaa tagaataaaa ccttttaggg
     6661 ctatgcactt gatgcccctt gccccatagc ttgcggtttc ctgccgccaa ccaactcatc
     6721 gccgcgcacc ggggcgagca ctaacgccct gttcgaccag ccgttgaaac aacagcccgc
     6781 gcgaacccga tgttcgccgg ttggaccgaa acgtgaactc atccaagtaa tagtctaaat
     6841 ggtccatctt caccgcgcct tgatgcgtcc cgagcaacca ccgttgcagc agcgccgcca
     6901 cccggttggc gcgtggcaac agattggcac caaccactgc actgg
//
LOCUS       DASZMM010000386         4538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_730577, whole genome shotgun sequence.
ACCESSION   DASZMM010000386 DASZMM010000000
VERSION     DASZMM010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730577"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VG754_09415"
     CDS             <1..319
                     /locus_tag="VG754_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF19477.1"
                     /translation="KTNAIQTAASTNPPANAATNETKISERKKSDEVAKPIEVVFVVD
                     GDHVKMVPVKRGIYDDNYVEITNGLKEGDQVVTGPYKALNRDLQEGTKVLVNSANFVA
                     KSN"
     gene            371..1012
                     /locus_tag="VG754_09420"
     CDS             371..1012
                     /locus_tag="VG754_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF19478.1"
                     /translation="MGAETIHALRDVSLDIQRGEYVAIMGPSGSGKSTLMNLLGCLDT
                     PTSGTYELNGTNVSEMDDNQLAEVRNREIGFVFQTFNLLPRSDALRNVELPLIYSGMD
                     ADERRQVALDSLASVGLADRIHHKPNEMSGGQRQRVAIARALVNKPSIILADEPTGNL
                     DSKTGEEIMALLDLLSKKGNTIFVVTHEEDVARRARRIIRIRDGLIASDELQK"
     gene            1059..1448
                     /locus_tag="VG754_09425"
     CDS             1059..1448
                     /locus_tag="VG754_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWF19479.1"
                     /translation="MQKEYDLMGNVIGFAMQVYRVLGPGFLESVYHKALEHELTKAGI
                     PFESKKQIIVWYDEIPVGEFEADFLVSGDFIVEIKAVQNLAMAHEIQTVNYLNAIRKD
                     TGLLLNFGAERLEFKKKFRKSAKAGIF"
     gene            1611..2843
                     /locus_tag="VG754_09430"
     CDS             1611..2843
                     /locus_tag="VG754_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF19480.1"
                     /translation="MNFFIELFEGLAIAWDAIRANKLRSALTTLGIIIGVATVTLMGT
                     AIDSMDRAFHKSISAIGADTLIINRIGWILNSDVDWEKMQRRNSITLEQVRAVEEQMT
                     HVIGVAPIVGTRQSVRYKDKMASGVQILGTSDQFLMASSFSLSDGHFITASDSRGGRP
                     VCVIGLEVATNLFPHEPAIGKQIRIGERRLEIIGVLEKQGSFLGIASLDNEVFIPVQQ
                     LLIGYYRNPDFTIRIKVTDVKDLDDDEEELRGIMRKARHVAPTDPDDFTINRQEQFIK
                     AFNSIAGTIAAVGLMITGLSLFVGGIGIMNIMFVSVAERTREIGVRKAIGAKRRNILI
                     QFLIEAATICLLGGLFALGVTYGATLLITRFFFTMNMSLPVMGLAVLVSLLTGLASGF
                     FPAWRAARMDPVEALRNE"
     gene            2845..4101
                     /locus_tag="VG754_09435"
     CDS             2845..4101
                     /locus_tag="VG754_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF19481.1"
                     /translation="MKRIHFLNLLLAEMRESFAMAASSLAAHKLRSALTLLGVMVGVF
                     SIIVVMTTMRAMQHYVEANMQQLGSETFVVQKWPGFSVRNNAKYWRRKDITFRQAMDV
                     QKRVSLPRYMGIEAAFDFDEIISDNGRAAPNANLVGENVGSFAVQNWILDDGRILQES
                     DLDGLRDVCILGSKMAKDLFPFGSPIDQVIKIDSIKYNVVGVLEPRGTMEGGNQDNFV
                     AVPLTTGLSRYGSQWYSLSILVQSPDHAHYDETVDQVRAALRVVRKVPPGDEDDFEIN
                     SNDSVMEQVNTFTFAVRMGVAAISSIALIAAGIGIMNIMLVSVTERTREIGIRRAIGA
                     KKRNIMTQFIMEAVVLCEIGGVIGVVFGVVGGDIGAHFMNLSPVIPVDWVILGLLICS
                     AVGIVFGTYPAYKAANLDPIDSLRYE"
     gene            4213..>4538
                     /locus_tag="VG754_09440"
     CDS             4213..>4538
                     /locus_tag="VG754_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HWF19482.1"
                     /translation="MSKTFNGPPTPEEFQRQMADFMRQHFQNAGINTAGKTDFESSAT
                     KPEKDSDKTDFEFDYKPRDIKEYLDRYVIKQDDAKKVLSVALCDHYHQVRLAFEGKET
                     PNYAKQ"
BASE COUNT         1108 a         1199 c         1222 g         1009 t
ORIGIN      
        1 caagaccaat gccatccaga ccgccgcctc aaccaatccg cctgcgaacg ccgccacgaa
       61 cgaaaccaaa atcagcgagc gaaaaaaatc cgacgaagtc gccaaaccca ttgaagtggt
      121 tttcgtggtg gacggcgacc acgtcaaaat ggtcccggtc aaacgcggca tttacgatga
      181 taattatgtg gagatcacca atggcctcaa agaaggagac caagtcgtga ccggcccgta
      241 caaagcgctt aatcgtgatc tgcaagaggg cacgaaagtc ttggtcaaca gcgcgaattt
      301 cgtggcgaaa tcgaattgaa ggccgaacgt gatcctcatt cgcctgcgaa atatttcccg
      361 ccgataccaa atgggtgccg agaccatcca cgcgcttcga gatgtctctc tggacattca
      421 acgcggcgaa tacgttgcca tcatggggcc atcgggttcg ggaaaatcca ccttgatgaa
      481 tctcctgggt tgccttgata cacccacgtc cggcacttac gaactcaacg gcacgaatgt
      541 cagcgaaatg gatgacaatc aactggccga agtccgcaat cgcgaaattg gttttgtgtt
      601 ccagacgttc aacctgctgc cgcgctctga cgcactgcgc aatgtcgagc ttcccttgat
      661 ttattccggc atggatgccg acgaacgcag gcaagtcgcg ttggattcgc tcgcaagcgt
      721 tggccttgcc gaccgcatcc atcacaagcc gaacgaaatg tctggcggtc agcggcagcg
      781 tgtggccatc gcgcgcgctc tcgtcaacaa accttcgatc attcttgcgg acgagccgac
      841 cggaaatctc gattcaaaaa ccggcgaaga aatcatggcc ttgctcgacc ttctttcaaa
      901 gaaaggaaac accatcttcg tggtgacaca cgaagaagac gttgcccgcc gcgcccgccg
      961 catcatccgc atccgcgacg gtttgatcgc cagcgatgaa ttgcaaaaat gagcgggaag
     1021 attcgacaga atgaacagaa ttttcagaat ggaaaactat gcaaaaggaa tatgacttga
     1081 tgggtaacgt gatcggcttc gcgatgcagg tttacagggt cttgggtcct ggttttcttg
     1141 agtctgttta ccacaaagcg cttgagcatg aattgacaaa agcaggaatc ccgttcgaat
     1201 cgaaaaagca aatcatagtt tggtatgatg aaattccagt cggcgaattt gaagctgatt
     1261 ttttggtgtc aggagacttc atcgttgaaa taaaggcggt ccaaaatttg gcaatggctc
     1321 acgaaatcca gaccgtcaat tatttgaacg cgatacgcaa agacaccggc cttttgctta
     1381 atttcggcgc tgaacgtctt gagtttaaga aaaaattccg gaaatccgca aaagcgggga
     1441 ttttttgaca gaatgaacag aatggacaga attcagaact gaaatcatgc aaaacgaata
     1501 tggattgacg ggaaaagtga tcagcttggt gaagcgcatc aacaccgtct tggggctatc
     1561 ttccaatttt attctgatca ttctgtccat tctgtcaaaa atcccctcct ttgaactttt
     1621 tcatcgagct ttttgaaggt cttgccatcg cgtgggacgc gatccgcgcg aacaagttgc
     1681 gctcggcctt gacgacgctt ggcatcatca tcggcgtggc caccgtcact ctgatgggca
     1741 cggccatcga cagcatggac cgcgcttttc acaaaagcat ttccgccatc ggcgcggaca
     1801 ctctcatcat caatcgcatc ggctggattc ttaattccga tgttgattgg gaaaaaatgc
     1861 agcgccgcaa ttccatcacc ctggagcaag tccgcgcagt agaggaacag atgacgcacg
     1921 tcatcggcgt cgccccaatc gttggcaccc gccaaagcgt tcgttataag gacaaaatgg
     1981 cctctggcgt ccagatcctc ggcacctcgg accaattcct tatggccagc agcttttcgc
     2041 tcagcgacgg tcatttcatc actgcctcgg actctcgcgg tggacggccc gtttgtgtca
     2101 tcgggttgga agtggcgacg aatcttttcc cgcacgagcc tgccatcggc aaacaaatcc
     2161 gcatcggcga acgccggctt gaaatcatcg gagttctgga aaagcagggg agcttccttg
     2221 gaatcgcgag cctcgacaac gaggtcttca tcccggtgca acaacttttg attggttatt
     2281 atcgcaaccc ggatttcacc atccgcatca aggtgacgga cgtgaaagat ttggacgacg
     2341 acgaggagga attgcgcggc atcatgcgca aagcgcgcca tgtcgccccg accgatcccg
     2401 atgatttcac catcaaccgc caggaacaat tcatcaaggc attcaacagc atcgccggga
     2461 ccatcgcggc ggttggattg atgatcaccg gattgtcatt gttcgtcggc ggaatcggca
     2521 tcatgaacat catgtttgtt tccgtcgcgg aacggacgcg cgaaatcgga gttcgcaagg
     2581 caatcggcgc gaagcggcgc aacattctga ttcaatttct gattgaagcc gcaaccatct
     2641 gcctgcttgg cggcttgttc gccctcggcg tcacttacgg agcgacgctg ctcatcacgc
     2701 gcttcttttt taccatgaac atgtccttgc cggtgatggg actggcggtg ttggtctcgc
     2761 tattgaccgg gctggcgtct ggttttttcc cggcatggcg cgccgcgcgg atggatcccg
     2821 tggaagcctt gcgaaatgag tgaaatgaaa agaatccatt ttctcaacct cctgcttgcg
     2881 gaaatgaggg aaagctttgc gatggccgca agctcccttg cggcgcacaa gctccgttca
     2941 gcgctcacgc tgttgggcgt gatggtcgga gtgttttcca tcattgtcgt gatgaccacc
     3001 atgcgcgcga tgcagcatta tgttgaagcc aatatgcagc agttgggcag cgagacgttc
     3061 gtggtgcaaa aatggccggg tttcagcgtg aggaacaacg ccaaatattg gcggcgaaag
     3121 gacatcacgt tccggcaggc gatggacgtt caaaagcggg tctcgttgcc gcgatacatg
     3181 ggtatcgagg cggcttttga ttttgacgaa atcatttccg ataacggcag agccgcgccg
     3241 aacgcaaatc ttgttggtga gaacgtcggc agttttgccg tgcaaaattg gatattggac
     3301 gacgggcgga tattgcagga gtccgatctg gacggtttgc gcgacgtttg cattctcggc
     3361 agcaagatgg cgaaagactt gttcccattc ggctcgccga tcgaccaggt catcaagatt
     3421 gacagcatca aatacaacgt cgtgggagtg ctggaaccgc gcggcacgat ggaaggcggc
     3481 aaccaggaca atttcgtggc cgtgccgctg accaccggcc tcagccgtta cgggagccaa
     3541 tggtacagcc tgagcattct tgtccagtcg cccgaccatg cgcattatga cgaaaccgtg
     3601 gaccaggtgc gcgccgcctt gcgcgttgtc cgcaaagtcc cgccgggcga tgaagacgat
     3661 ttcgagatca attccaacga ctcggtcatg gagcaggtca atactttcac cttcgccgtc
     3721 cgcatgggag tcgcggccat cagttccatc gcattgattg ccgcgggcat cggcatcatg
     3781 aacatcatgc tcgtctctgt gactgagagg acgcgtgaaa tcggcattcg ccgcgccatc
     3841 ggcgcaaaaa agcgaaacat catgacgcaa tttatcatgg aagccgtggt gctctgcgaa
     3901 atcggcggcg tcattggcgt ggtgttcgga gtggtgggcg gcgacatcgg cgcgcatttc
     3961 atgaatttat cgccggtgat tccagttgat tgggtgatac tcggcttgct gatctgctcg
     4021 gcggtgggaa tcgtgttcgg cacttatccc gcatacaagg ccgcgaacct ggatcccatt
     4081 gattccttgc ggtacgagtg agcgttcaaa tgctcgtttt ggcagggctc gacatatcgg
     4141 ccaaatggac tagtttaaaa catgcgccac ggcgcacaat tttcgacgaa gccatttcgc
     4201 aaccacaatt ttatgagcaa gacttttaat ggtccgccca cgccggaaga atttcaacgg
     4261 cagatggcgg acttcatgcg ccagcatttt caaaacgccg gaatcaacac cgccgggaaa
     4321 accgatttcg aaagttccgc gaccaagccg gagaaggatt ccgacaagac cgatttcgaa
     4381 tttgattaca aaccgcgcga catcaaggaa tatttggacc gctacgtcat caagcaggat
     4441 gacgccaaga aagtcctgag cgtggcgttg tgcgaccatt atcatcaggt gcggctcgca
     4501 ttcgagggca aggaaactcc gaattacgcg aagcaaaa
//
LOCUS       DASZMM010000387         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_453346, whole genome shotgun sequence.
ACCESSION   DASZMM010000387 DASZMM010000000
VERSION     DASZMM010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453346"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VG754_09445"
     CDS             complement(<1..1015)
                     /locus_tag="VG754_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19483.1"
                     /translation="MKGKRLITCGLLALLLLVAVNALIARAARVSLRRQFLTRLEALP
                     QNVDCMFLGNSLVEAGCDANAFIAAWPDKKHPPVAVNLGLGATTPVEHYLVLKQALAR
                     CPHPKYLIYGFFDDQLNQTVNGRYCDLVGNRAFSYYFPEDAAAFYTPGSRFEAWKMRV
                     IGHIPMLAERSSLWGKVELLRRRISEIGMPHQKINRFGRVSDFANLEAQDVPSFIQRC
                     ESIVRERKDFSPPIREILRLAREHGVTVFLVEMPMPTQHRKLFYSLPAWQDLRAHLQT
                     LATREHAYYIPASDWVADDNKFEDAVHLNERGAAFFSDALAPSITAVEQHQTPTFLHA
                     SQST"
     gene            complement(1017..1202)
                     /locus_tag="VG754_09450"
     CDS             complement(1017..1202)
                     /locus_tag="VG754_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19484.1"
                     /translation="MKFLRTIKSFCDYIGGSGENPPPEIGWLISQWWWRGLWWGMLAI
                     VICAFCGQTSKFIYIDF"
     gene            complement(1207..2388)
                     /locus_tag="VG754_09455"
     CDS             complement(1207..2388)
                     /locus_tag="VG754_09455"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HWF19485.1"
                     /translation="MIFNTIEYFLMFLVPAAILFRLVKPSLQPWVCLGFGAAFFVFFS
                     LTQIGGAAGAACLLIFIWESLFSRLYRPGSWLCFIGIVQALVLLMVFKYWNFFTSLCF
                     GHAGHNPFYWYGAFLPLGISFFTFEFIHYAVDRYRGTTPAGKFSEYLAFILFFPTMVA
                     GPIKRYGDFLPKLCQPSLDWEEDWQRGVTRILVGLAKKFLIADLLTAFTQHLCRNDIA
                     QAQRWVLPVWLLAFGAQIYFDFSAYSDIAIGSARLFGIKVLENFDWPYLRTNIAEFWR
                     HWHMSLYRWLVDYVFIPLGGSRVRPARVYVNLLITMFLSGLWHGAGLNFLVWGLWHGM
                     LLSIHRLWKRWRGESELPPARSAQIAGWAITFVAVTVGWAFFAMDLHTSVYYFHRLFL
                     G"
BASE COUNT          631 a          709 c          731 g          449 t
ORIGIN      
        1 ccgttgattg gctggcatga aggaacgtgg gtgtttgatg ttgttcaacc gccgttatcg
       61 aaggcgcgag ggcatcgctg aaaaacgcgg ccccgcgctc gttcaaatgg actgcgtcct
      121 cgaatttgtt gtcatccgcg acccaatcgc tcgcaggaat gtaataggca tgttcgcggg
      181 tcgcgagggt ttgcagatgc gcgcgcaaat cctgccacgc gggaagcgaa taaaaaagtt
      241 tgcgatgctg cgttggcatg ggcatctcga cgaggaacac cgtgacgccg tgctcgcgcg
      301 cgaggcggag aatttcacgg atcggcggcg aaaaatcttt gcgttcgcga acgatgcttt
      361 cgcagcgttg gatgaaagag ggcacgtcct gggcttcgag attcgcaaaa tccgacacgc
      421 gcccgaagcg attgattttt tgatgcggca ttccgatttc actgatgcgc cgccgcagca
      481 actccacttt tccccaaagc gaactgcgtt ccgcaagcat cggtatgtgg ccgatgacgc
      541 gcatcttcca tgcttcaaag cgcgaaccgg gcgtgtaaaa cgcggcggcg tcctcgggaa
      601 aatagtagga aaaagcgcgg ttgccgacga ggtcacaata gcgtccgttc acagtctggt
      661 tgagctggtc gtcaaaaaat ccgtagatga gatatttcgg atgcggacag cgggcgagcg
      721 cctgtttcaa cacgagataa tgttccaccg gcgtcgttgc gcccaaccca agattcacgg
      781 caacgggcgg atgcttcttg tcgggccatg cggcgataaa ggcgttggcg tcgcagcccg
      841 cttcgacgag tgaattgccg aggaacatgc aatccacgtt ttgcggcagg gcttcgaggc
      901 gcgtgaggaa ttggcggcgc aacgagacgc gggcggcgcg ggcgatgagc gcattgactg
      961 ccaccaacag cagcaacgcc agcagtccgc aagtgatgag ccgtttgcct ttcatctcag
     1021 aaatcgatgt agatgaactt cgatgtctgc ccgcaaaacg cgcaaatgac aatcgcaagc
     1081 attccccacc agaggccgcg ccaccaccat tgggaaatga gccagccgat ttcgggcgga
     1141 gggttttcac cgctgccgcc gatgtaatcg caaaatgatt ttatcgttcg caaaaatttc
     1201 atggcatcat cccaaaaata aacggtggaa ataataaacg ctggtgtgca ggtccatcgc
     1261 gaagaacgcc cagccgacag tcacggcaac gaacgtgatt gcccagccgg cgatttgcgc
     1321 gctgcgcgcg ggcggcagtt ccgattcgcc gcgccagcgt ttccagaggc gatggatgga
     1381 gagcagcatt ccgtgccaca agccccacac caaaaagttc aaacccgcgc cgtgccatag
     1441 cccgctgaga aacatcgtga tcagcagatt cacatacacg cgcgcggggc gaacgcgcga
     1501 gccgccgagc gggatgaaca cataatccac cagccagcga tacagcgaca tgtgccagtg
     1561 gcgccaaaat tccgcgatgt tcgtgcgcaa ataaggccag tcaaaatttt ccagcacctt
     1621 gatgccgaac agccgcgccg agccgatggc gatgtccgag taagccgaga agtcgaaata
     1681 aatctgcgcg ccgaacgcca gcaaccacac cggcaacacc caccgttgcg cctgcgcgat
     1741 gtcgttccgg caaaggtgtt gtgtgaacgc ggtgagcagg tccgcaatca ggaatttttt
     1801 ggcaagcccg acgagaatgc gcgtcacgcc gcgctgccag tcttcttccc aatccaaact
     1861 tggctggcag agcttgggaa gaaaatcgcc gtaacgcttg atcggcccgg cgaccatcgt
     1921 cgggaaaaac aggatgaacg ccagatattc ggaaaatttt ccggcgggcg tggtgccgcg
     1981 gtagcggtcc acggcgtaat ggatgaattc aaaggtgaag aacgagatgc ccagcggaag
     2041 aaacgctccg taccaataaa atggattgtg cccggcgtgt ccgaagcaaa ggctggtgaa
     2101 aaaattccaa tacttgaaaa ccatgagcag caccaatgcc tgcacaatgc cgatgaaaca
     2161 caaccatgag ccgggccgat aaagccggct gaacagcgat tcccagatga atatcaacag
     2221 gcaggccgct cctgccgcgc cgccgatttg ggtcagcgaa aaaaagacaa agaaagccgc
     2281 gccgaaacca aggcagaccc acggttggag cgaaggcttc accagccgga acaaaatcgc
     2341 ggcgggcacc aggaacatca gaaaatactc aatggtattg aagatcataa cccgcgtggc
     2401 gtggatacga cgcgaaagtt ttaccccatc gcgcgcgcac cagcctatcg ggcgaatccc
     2461 ccaatttggc cccgtctgaa ccttacatgg agcgcggctg tgcgcgaagc gccagcctcc
//
LOCUS       DASZMM010000388        10378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4834211, whole genome shotgun sequence.
ACCESSION   DASZMM010000388 DASZMM010000000
VERSION     DASZMM010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10378
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4834211"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="VG754_09460"
     CDS             <1..107
                     /locus_tag="VG754_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HWF19486.1"
                     /translation="RRLLKQVAAGAEIKGDTTTLEDFGVLAKLSSSEE"
     gene            385..1608
                     /locus_tag="VG754_09465"
     CDS             385..1608
                     /locus_tag="VG754_09465"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWF19487.1"
                     /translation="MPEPRPLRHAREASPLPVYDPSAAQLDVAREILKDSPIIDGHND
                     TPWQFRKRGNDMTAIDLATNTSGLKMVTDIPRLRAGCVGGQFWSVYIPSTMGGSIAVH
                     AVLEQIDVVHEMVARWPDTFELALTADDIERIERKGKIASLIGMEGGHSIDNSLAILR
                     MTYALGARYMTLTHVKNTDWADSANDEPKHHGLTPFGEDVIREMNRLGMMVDLSHVSA
                     ETMQAVLKISKAPVIFSHSSARALCDNPRNVPDEILRQMPTNGGIVMINFFPEYLNER
                     ANQHLTAQKHEWERLGKLYPDNTNQVRALMKEWKAAHPMPHLATLNDVADHIDYVRKV
                     AGIDYVGIGSDFEGFDGPPDGLEDVSCYPALMAELLRRGYTPDDVKKVSGRNLLRVFR
                     QVEKVAAQSQHESQR"
     gene            complement(1643..1894)
                     /locus_tag="VG754_09470"
     CDS             complement(1643..1894)
                     /locus_tag="VG754_09470"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HWF19488.1"
                     /translation="MDAKSQAEGLKQAADDTMEDLQETGEDIQNRLSTLLEAGREKAG
                     YYARNADQKIRDNPYQAVGIAFGLGLIVGLLLTRRGSED"
     gene            2110..2186
                     /locus_tag="VG754_09475"
     tRNA            2110..2186
                     /locus_tag="VG754_09475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2144..2146,aa:Arg,seq:acg)
     gene            2281..7263
                     /locus_tag="VG754_09480"
     CDS             2281..7263
                     /locus_tag="VG754_09480"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HWF19489.1"
                     /translation="MNPPFAFKFWRCAGSTLVIAMTILLAGSVATQARMIDQNGNGMS
                     DVWEWLYNANGINPNQDSDGDGFSNLQEALAGTNPFDSNSFPHITFASGNSNAFTVTI
                     PCAWGKNYQLQSAAAPVSTNWVIETNIEATAGATITLTAPVSTTMRFYRVAISDVSSD
                     GVGLTDWEAYQLGLNPANGFSNGQEDSNGNAMNDYNYAVAKLAQQNVITISATGPTAT
                     QPDPGQGVTAPGQFTITRGGFPLTSLTINLATNGPGPGFGTPGVDFVSLPTAIIFPAG
                     TSSMTFPLTPMPNTNLQAPVLAQWKLMPGSHYTLGTASNASVVVYPSPTSKGAGLTGQ
                     YYTNSSTTYTNAANFNPTNLFLTRIDPTIDFNWTNGTSPDLSNGLYTVRWTGQVQPQF
                     SETYIFDVKSDDGCRLWVNDQLLIDKWQSQGVTDWTNSITLVAGARYDIKLDYLQTGG
                     AAQAHLYWYSPSQPEVIIPNNCLYPSNSFVTNATNAASVVTSALTAVAFLGQPFTFTV
                     TAANVPLGFTASGLPPGLSFNTNNGLISGVPTLAGNFLVSLTSSNHAGIGASTLAITV
                     FNTGSSVVQEIWTNVPGMSIASIPTGTPANMTNVLGTLEGSTDYGNNYGERIRGFFTA
                     PVTGNYYFWIAGSDSAQLWISDDGDPVDKVLRASVAPTNNPSSPPQYGTSSHQWTVQS
                     GQQSAWLTLAAGQQYYVEILHKVGVDSSNNWSVAWLQDPTGTNNTPGGGVVPGYLLSR
                     YYPPLPVNVAGTLYSANMLALTGVNSQGVGSATLRVSADGSTATLNFQIANMVGMPTG
                     ESINSDPYLSDPGELVYDISASKPQPDGSYLWKLEATGPLAVSDIQEIIREGKASIVI
                     ESSAFPNGEITGHFTPANGAGSFAPPPAPPAWTDDSANTNAAVRFLTQATFGASPSDI
                     TNVQALGYAGWISNQFSLPATHHLPYVQGNPYSDPTDLYQSPLTFASWWQKSVTAPDQ
                     LRQRVAFALSEIVVVSENGVLQNHADGLSSYYDMLLDNAFGNFRNLLERVTLHPAMGL
                     YLNMQGNSAGSIITGVHADENYAREIQQLFSIGLNRMWPDGTLILNAQGNLVPTYNQN
                     VIMGFASVFTGWTYYQTNQANGRLPSNFFPPANYTNYMVLVPTHHELGAKLLLDNVML
                     PAAQGASANSSLTNYDYYCSQDLEQALNTIFNNQNVAPFICRELIQRLVTSNPSRDYV
                     YRVASVFNDDGTGVRGNMQAVVQAILLDYEARSPAMIPQPDYGKQREPLVRVTSLARA
                     FPAPAIINGTYTQTTNQVISITNSSPHRMGSGDVAFLTFTDTSGYSAPTSQGYTVTVV
                     NPTNFTVNAPQLLSGSYGQTNGVITLNLSGNGLASNNPVYLVFTTGGASNGLYSVSNV
                     IDSAHFTVAAIETNTRSGNCLLPKISAGGYTQTGTTILISTSGNHGLVAGNNVYINFS
                     SGTAVDGTYTIATVPNPTHFTITAGTSANQNQNILTVYSLTAPQLARSGTVTIRYDTW
                     NMGYTDSGSSSSLMETPLRSPTVFNFYHPEYEFPGALASAGMTTPEFELTTDSGVSSQ
                     MNFLESGILNNTGNTNGLSSFNSGSIVLDIGPWMTPAYTSNAGIPALVSNLNTLLAAG
                     QLSASAQSSIVSYVASTNFSYTTPTYTQMRDRVRAVVHQIVNSPDYMIQK"
     gene            7268..8767
                     /locus_tag="VG754_09485"
     CDS             7268..8767
                     /locus_tag="VG754_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF19490.1"
                     /translation="MNDKNISVLTRRDFIRRAACAAVGTAAMTSAIRDLRFMNAAVAQ
                     SNITDYKALVCIFLQGGNDSNNLIIPAIQSEYNNYASIRTPVLAIPQSALLTLMNGSN
                     PYVDPAGHEFGLHPSCPELQTLFSEQKLAVLFNAGTLLYPITRSQYLSGSKAKPPQLF
                     SHADQQTQWQTSVPDQPPTTGWGGRCADLLNAVQPGAPISLMVTLAGANTFEIGNIVA
                     QYSVSTSGAISLSLPGTPGGGASSNRLPTLMNLLGLPYTNLQSQAYAGVAEHAINTSS
                     LLNTAIAPTGGSYWTTPFPTKITPPESGAAAFTSSLSPQLQMIARLIAAGNTPTSSGG
                     FGMKRQIFFCQVGGYDLHSGQTNYSTGTPNNVLLGAHTNLLAELSQSMFAFQRAMEQL
                     GISQEVTCFTASDFGRTFPSNGQGSDHGWGSHHLVLGGAVKGRQTYGSFPALSVNGPN
                     DTSTGRWIPTTAIDQYFATLATWFGVDSGNLTTILPNIGRFSSSNLGFI"
     gene            8767..9366
                     /locus_tag="VG754_09490"
     CDS             8767..9366
                     /locus_tag="VG754_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19491.1"
                     /translation="MKRFFTVILACLLAGVVFLVFIRTQHVSPPSPEENLDQQSSSNQ
                     AASPAGNAASTIPADSSSAGTGTANPSSPNASSATQPENLSARAGERVDETNEGVNLP
                     PDIVLQNVRRAVRQYGAMFGGNPVGTNPEITAALTGKNPKQINFLNGQPGMRINQDGE
                     LLDAWGTPYFFHQISGTEMEIHSAGPDKIMWTSDDLVTR"
     gene            9476..>10378
                     /locus_tag="VG754_09495"
     CDS             9476..>10378
                     /locus_tag="VG754_09495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HWF19492.1"
                     /translation="MLVTKTENGLFCEQGNFYIDPWKPVDAAVITHAHSDHARIGSRY
                     YLTETSGKSILQQRLGPDARIETLPYGESVSRNDVKISLHPAGHILGSAQVRIESGGE
                     VCVVSGDYKLEHDGICQPFEPVRCHMFVTESTFGLPIYHWRPQAEIFAEINDWWRENR
                     SRERTSVIFCYALGKAQRILSGLDASIGPIFLHGAMERFMPAYHQAGVQFPATTRATA
                     ELVKGAGGRAVVLAPTSADNSSWLRKFGAVSTAFTSGWMQIRGTRRRRSLDRGFVLSD
                     HADWTGLLETIDATGAETVWATHGY"
BASE COUNT         2346 a         3057 c         2698 g         2277 t
ORIGIN      
        1 tgcggcgctt gttgaagcag gtcgccgccg gcgcggagat caagggcgac accacgacgc
       61 tggaagattt cggcgtgctg gccaaactga gcagcagcga ggagtgacat gaaacgcagg
      121 ccaaacagct tgctttgtcg agccggttct gcgttcatat caatccatct tgcagttgaa
      181 cgccttttcc cgagcgacat gaaacgcgac cgcatctttc ttttattgct tatgctgaca
      241 ctggccaaac cgtctgttgg gtcggctcag ggacaagcag cagggccgag ttcacgaaat
      301 acaatggtcc cagcgccgct tcctccgcca cttacgagat ctaactcact agtgcgctct
      361 ggaaccgcac agcaatcaac ttttatgcct gaaccgcgtc ctttacgcca tgcgagagaa
      421 gcgagtcctt tacctgtgta cgacccgagc gcagcccaac tcgacgtggc ccgggaaatc
      481 ttgaaggatt ctcccatcat tgacggccat aacgacacac cctggcaatt ccgcaagcgc
      541 ggcaatgaca tgaccgcgat tgatctcgcc acaaacacga gcggattgaa aatggtcact
      601 gatattccac ggttgcgcgc gggatgtgtc ggcgggcaat tctggtcggt ttacattcct
      661 tcgacgatgg gcggttccat cgccgttcac gcggtgctcg aacaaatcga cgtggtgcat
      721 gagatggtcg cgcgttggcc ggatactttt gaactggcgc tcacggcgga tgacatcgag
      781 cggattgagc gcaagggaaa aattgcctcg ttgatcggca tggaaggcgg gcattcgatt
      841 gacaattcgc tggccatcct gcgcatgacc tacgctttgg gcgcgcgtta catgacgctc
      901 acgcacgtca aaaatacgga ttgggccgat tcggcgaacg acgagcccaa gcatcatggc
      961 ttgacgccgt tcggcgaaga tgtcatccgc gaaatgaacc gtctcggcat gatggtggac
     1021 ttgtcgcatg tctcggccga gacgatgcag gcagtcttga aaatctcaaa ggcgcctgtg
     1081 attttttcgc attcgtccgc tcgcgcgctt tgcgacaatc cgcgaaatgt gcccgatgaa
     1141 attcttcgcc aaatgccgac gaacggcggc atagtcatga tcaatttttt cccggaatac
     1201 ctgaacgagc gggcgaacca gcatctgacc gcgcagaagc acgaatggga gcggttgggc
     1261 aagctttacc cggacaacac caaccaggtc agggcattaa tgaaggaatg gaaagccgcg
     1321 catccaatgc cgcatctggc gacgttaaac gatgtggccg accatattga ttatgtgcgc
     1381 aaggtggccg ggattgatta cgtcggcatc gggagcgact tcgaagggtt cgacgggccc
     1441 cccgatggat tggaggacgt atcgtgttac ccggcgctga tggcagaatt attgcgccgc
     1501 ggttacacgc ctgacgacgt caagaaggtt tccggacgga atcttttgcg cgtgttccgc
     1561 caggtcgaaa aagtggcggc ccaatcgcaa catgaaagcc agcgctgacg cgtgcaaccg
     1621 ggcgccgctc gcgcgaaacg gtttaatcct cgcttccgcg ccgggtgagc agaagaccca
     1681 cgatcaatcc caagccaaag gcgatgccga cagcttgata agggttgtcg cgaatttttt
     1741 ggtcggcgtt gcgcgcgtaa taaccggcct tctcgcgccc ggcttccaac agggtcgaga
     1801 ggcggttttg gatatcttcg cccgtttcct ggaggtcttc catggtgtcg tctgcggcct
     1861 gcttgaggcc ttctgcttgt gatttggcat ccatatattg ttcctttgga gcgtgtgatt
     1921 tttgtttatg agacagccaa tcgagtttcc acggcagctc acaaagagaa taaccggcac
     1981 aacggcaaaa accatggggt gtaatgctaa gcaatttgaa aaaacttcga cggcatggac
     2041 atggacatca gcgtaataaa agacttgcgc gcaaggcatg tgatttgtag gtttcaacgc
     2101 tcttcggttg cacccatagc tcaattggat agagcgtctg actacggatc agaaggttag
     2161 aggttcaact cctcttgggt gcaccagttg cgtgttttca aaaccgtaac actcttcgtt
     2221 cttgacattc ctgctgcttt ggagaattat ctgcgctaac gcatcctaac agagtctcac
     2281 atgaatcccc cttttgcgtt taagttctgg cgttgcgcag gttccacgct ggtcatcgca
     2341 atgacgattc tcctcgccgg atcagttgcc acgcaggcac gcatgattga tcagaacggc
     2401 aacgggatga gcgatgtctg ggaatggctt tacaacgcga acggcatcaa tcccaaccag
     2461 gattcggacg gcgacggttt ttcaaatctt caggaagcgc ttgccgggac gaatccgttc
     2521 gactcgaatt ccttcccaca catcacgttt gcctcgggca attcgaacgc ctttacggtg
     2581 accattccat gcgcgtgggg aaagaactac caattgcaaa gcgcagcagc tccggtcagc
     2641 accaattggg tgatcgaaac caatattgaa gcgaccgccg gggcaaccat tacgttgact
     2701 gcgcctgtgt caacaacgat gcggttttat cgcgtcgcta tttccgatgt gagcagcgat
     2761 ggcgttggcc tgactgattg ggaagcctat caactcggac tcaacccggc caatggcttc
     2821 agcaatggcc aggaggattc caacggcaat gccatgaatg attacaatta cgccgttgcc
     2881 aaactggcgc agcaaaatgt gatcaccatc tctgccaccg gcccgacagc cacgcagccc
     2941 gatcccggcc aaggcgtgac ggcacccggg cagttcacca tcacccgtgg cgggtttcca
     3001 ttgacttcgc tgacaattaa tctcgccaca aacggccccg gtcccggatt tggcacgccc
     3061 ggcgtcgatt ttgtctcact gccaacagcg attatctttc cggcgggcac cagttccatg
     3121 acattcccgc tcacaccgat gcccaacaca aatttacagg cgccggtcct ggcgcaatgg
     3181 aagctcatgc cgggatctca ttatactttg ggaaccgcca gcaacgcgag tgtggtcgtt
     3241 tatccttccc cgacttcaaa gggcgccgga ttgaccgggc aatattatac gaactcgagc
     3301 acgacttaca ccaacgcggc caatttcaat cccacgaatc ttttcctgac gcgaattgat
     3361 ccaacgattg atttcaactg gacgaatggc acctcgccgg acctgagcaa cgggctctac
     3421 acagtgcgtt ggacaggcca ggtccaaccg cagttttccg aaacttacat attcgacgtg
     3481 aaaagcgacg atggttgccg tttgtgggtc aatgaccagt tgttgataga taaatggcag
     3541 tcgcagggcg tcacggattg gacgaattcg atcaccttgg ttgctggcgc gcgttacgat
     3601 atcaagctgg attatctgca aacgggtggc gcagcccagg cgcatttgta ttggtacagc
     3661 cccagtcaac cggaagtgat aattcccaac aactgtttgt atccttcaaa ctcttttgtc
     3721 accaacgcaa ccaacgccgc ttccgtggtg accagcgcat tgaccgctgt cgcgtttctc
     3781 ggtcagccgt tcacattcac ggtcacagcc gcgaatgtcc cgctcggttt cacggcgagc
     3841 ggcctgcctc ccggcctaag ttttaacacc aacaacgggc tcatcagcgg cgtgcccact
     3901 cttgcgggca atttccttgt ttcgctcacg tccagcaatc atgccggcat cggcgcatcc
     3961 accctggcga tcactgtttt caacaccggc agttcggtcg tgcaggaaat ttggacgaac
     4021 gtgcccggca tgagtatcgc cagcattccc accggcacgc ccgcgaacat gaccaacgtg
     4081 cttggcacat tggaaggcag cactgattac ggtaacaatt acggcgaacg catccgcgga
     4141 tttttcaccg cgccggtcac gggaaattat tatttctgga tcgcaggaag cgattccgcg
     4201 caactttgga tttctgacga cggcgatcca gttgataaag ttttgcgcgc ctcggttgcg
     4261 ccaacgaata atccttcctc gccgccgcaa tacggaacca gttcgcacca atggaccgtg
     4321 caatccggcc agcaatcagc gtggctcacc ctcgcagccg gccagcaata ttacgtcgaa
     4381 atcctgcaca aggttggcgt tgactccagc aacaactggt cggtcgcgtg gctgcaagac
     4441 ccgacgggca ccaacaacac gcccggtggc ggcgttgtgc cgggctatct tttgtcccgt
     4501 tattatccgc cattgccggt gaatgtcgcg ggcacgcttt attccgcgaa catgctcgcg
     4561 ctgaccggcg tcaacagcca gggggttggc tcggcgacgt tgcgcgtgag cgcggatggt
     4621 tcgacggcga ctttgaattt tcaaatcgcc aatatggttg gaatgccgac cggcgaatcc
     4681 atcaattccg atccctacct gagtgacccc ggcgaattgg tttacgacat ttccgcctcc
     4741 aaaccgcagc ctgacggcag ttatctttgg aagctggaag ccaccgggcc gctcgccgtt
     4801 tcagacatcc aggagatcat cagggaaggc aaagcttcca ttgtcatcga aagctcggca
     4861 ttccccaacg gcgaaatcac ggggcatttc acgccggcaa atggagccgg atcgttcgct
     4921 ccgccgcccg cgccgcccgc gtggacggat gattccgcga acacaaacgc cgccgttcgc
     4981 tttttgacgc aggccacgtt tggcgcttca cccagcgaca tcaccaacgt ccaggcgctc
     5041 ggttatgcgg gttggatctc aaatcaattt tcgctgccgg ccacgcatca tctcccgtat
     5101 gttcaaggta atccctattc cgacccgacc gacctgtatc aaagcccgct cacattcgca
     5161 tcttggtggc agaaatcggt caccgcgccg gaccaattgc gccagcgcgt cgcgtttgcg
     5221 ttgagtgaaa tcgttgtcgt ttcggaaaac ggcgttttac aaaaccatgc cgatggcctc
     5281 tcgtcttatt acgacatgtt gctcgacaat gcgttcggca atttccgcaa cctgcttgaa
     5341 cgcgtcaccc tgcatccggc gatgggcctt tacctcaaca tgcagggcaa cagcgcgggc
     5401 agcatcatca ccggcgttca tgcggatgaa aattatgcgc gtgaaatcca gcagttgttt
     5461 tccatcggat tgaaccgcat gtggccggac ggcaccctca tcctgaatgc gcagggcaat
     5521 ctcgtgccaa cctacaatca gaatgtcatc atgggcttcg ccagcgtttt cacaggctgg
     5581 acttactatc agaccaatca agccaatggc cgcctgccgt ccaacttttt tccgccggcg
     5641 aattatacca attacatggt tctggtcccg acgcatcatg aactcggcgc caagctcctg
     5701 ctcgacaatg tcatgttgcc cgcagcccaa ggcgcttccg caaattccag cctcacgaat
     5761 tacgattact attgctcaca agatttggaa caggcgttga acaccatctt caacaatcaa
     5821 aacgtcgcgc cgtttatctg ccgcgaattg atccagagac tcgtcaccag caacccgagc
     5881 cgtgattatg tctatcgtgt cgcttccgtg ttcaatgacg atggaaccgg cgtgcgaggc
     5941 aatatgcagg cggtcgttca agcaattctg ctcgattacg aagcgcgcag cccggccatg
     6001 attccgcagc ccgattatgg caaacagcgt gagccgttgg tgcgcgtcac gtcactggca
     6061 cgtgcatttc ctgcgcctgc gataatcaat ggcacttaca cccagaccac aaaccaggtc
     6121 atcagcatca caaactccag cccgcatcgc atgggcagtg gtgacgtcgc gttcctgact
     6181 ttcaccgaca catccggtta ctccgccccc acgtcgcagg gttacaccgt gaccgtcgtc
     6241 aatccaacca actttactgt caatgcgccg caattattgt ccggctctta cggccagacc
     6301 aacggcgtca tcacgctcaa tctttccggg aacggcctgg cgagcaacaa tccggtctat
     6361 ttggtgttca ccaccggggg cgcttcgaac ggactttatt ccgtgtccaa tgtcattgat
     6421 tcggcccatt tcacggtcgc cgccattgaa acaaacaccc gttccggcaa ttgtctgctg
     6481 ccaaaaatct ccgccggcgg ttacacgcaa acggggacca ccattctcat cagcacatct
     6541 ggcaatcacg gtttggttgc gggcaacaat gtatatatca acttcagctc cggcacagcg
     6601 gtggacggaa cttacaccat cgcgaccgtt cctaacccaa cgcacttcac gatcaccgct
     6661 ggaacgtcag caaaccaaaa tcaaaacatc cttacagttt attcattgac cgcgccgcaa
     6721 ttggcccgtt ccggaaccgt caccatccgg tacgacacat ggaacatggg ctacaccgat
     6781 tccggcagca gttcgagcct gatggaaaca ccgttgcgct ccccgacagt attcaatttc
     6841 tatcatcccg agtatgaatt cccgggcgcg ttggcctcgg cgggaatgac gactccggaa
     6901 ttcgagttga ccacggacag tggcgtctca tcgcagatga atttcctcga aagcggcatc
     6961 cttaacaaca cagggaacac caatggactg agcagcttca acagcggctc catcgtgctc
     7021 gacatcggac catggatgac tcccgcttac acttccaatg cgggcattcc ggcgcttgtg
     7081 agcaatctca acactttgct tgctgccgga caattgtccg catcggcgca atcaagcatc
     7141 gtcagctatg tggccagcac caatttcagc tataccactc cgacttacac gcaaatgcgg
     7201 gatcgtgttc gcgcagtcgt tcaccagatt gtcaactcgc cggattacat gatccaaaaa
     7261 tgattttatg aacgataaaa acatttcagt cctgacaagg cgtgatttca tacgccgcgc
     7321 cgcgtgcgct gccgtcggga ctgccgcgat gacttctgcc atccgggatt tgcggttcat
     7381 gaacgccgcc gtggcgcaat ccaacatcac ggattacaag gcgctggttt gcatttttct
     7441 tcagggcgga aacgactcca ataacctcat catacccgcc atccagtcgg agtacaacaa
     7501 ttacgcctcc atccgcacgc cggtgcttgc gattccacaa agcgctttgc tcaccttgat
     7561 gaacggctcg aacccgtatg tggaccccgc aggtcacgag ttcggcctgc atccgagctg
     7621 cccggaattg caaacacttt tcagcgaaca gaagcttgcc gtccttttca acgccggcac
     7681 cctcctttat ccaatcaccc gctcgcaata tctctccggc agcaaagcca agccgccgca
     7741 gcttttttcg cacgcagacc agcagacgca gtggcaaacc tccgtcccgg accaaccgcc
     7801 cacaaccgga tggggcggac gttgtgcaga cttgttgaat gccgtccagc cgggagcccc
     7861 gatttcgttg atggtgactc tagcgggtgc gaacacgttt gaaataggca acatcgtcgc
     7921 gcaatattca gtttcgacga gcggagccat atcgttgtcg ctcccgggta ctccaggcgg
     7981 cggcgcgagt tcaaatcgcc tgccaacatt gatgaacctt ctcggcctgc catatacgaa
     8041 tttacaatca caagcttacg caggggtggc cgaacacgcc atcaacacca gctcgctgtt
     8101 gaacactgcg attgctccaa ccggaggaag ttattggacc acgccgttcc cgaccaaaat
     8161 caccccgcct gaaagtggcg cggcggcctt tacctcctca ttaagcccgc aacttcaaat
     8221 gattgcgcga ctgatcgcgg cgggaaacac gccgacttcc agcggcggct ttggcatgaa
     8281 acgccagatt tttttctgcc aggtcggcgg ctatgacttg cacagcggcc agacaaatta
     8341 cagcactggc acgcccaaca atgtcctcct cggcgctcac accaatctgc tcgcggaatt
     8401 gagccagagc atgtttgcct ttcaacgcgc catggaacag cttgggattt cccaggaggt
     8461 cacttgtttc accgcgagcg attttgggcg cacttttccg agcaacggcc agggcagcga
     8521 tcacggttgg gggagccatc acctcgtcct tggcggtgcg gtcaaaggcc ggcaaactta
     8581 tggctccttc cccgcgcttt ccgtcaatgg accgaacgac accagcacgg ggcgttggat
     8641 tccaacgacg gccattgacc aatactttgc cactttggca acgtggttcg gtgtggacag
     8701 cggcaacctg acgaccatcc ttccgaacat cggtcgcttc tccagctcaa atctcggttt
     8761 catttgatga aacgtttttt cacggtcatc ctggcgtgtc tgctggctgg cgtcgttttt
     8821 ctcgtattca ttcggacgca acacgtttcg ccgccgtcgc ctgaagaaaa tttagatcag
     8881 caaagttcca gtaatcaggc cgcatctccg gcgggcaatg cagcttcaac aattccggca
     8941 gattcctcct ccgcaggaac tggcactgcg aatccgtcat caccgaacgc atcatccgca
     9001 acacagccag aaaacctctc ggctcgcgcg ggtgaacgcg tggatgagac caacgaaggc
     9061 gtgaatcttc cgccggacat cgtcctgcaa aatgtccggc gcgcagtccg tcaatatggc
     9121 gcgatgttcg gcggaaatcc ggtcggcaca aatccagaaa tcaccgctgc gctcaccggc
     9181 aaaaatccga aacaaatcaa cttcctgaat gggcaaccgg gaatgcgcat caatcaggac
     9241 ggcgaactgt tggacgcatg gggcacgcct tatttcttcc accaaatctc agggaccgaa
     9301 atggaaatcc attccgccgg acccgataaa atcatgtgga cgtccgacga tttggtcacg
     9361 aggtaaacgc gctgtcccgc aaagcaagat caaaggaggc cacttttccc ctcaaaggcg
     9421 caatgcggac aattgagcta tattgaattc ccggcttgga aactgccggg aaaagatgct
     9481 ggtaacgaaa actgaaaacg gacttttttg cgaacagggc aatttttaca ttgatccttg
     9541 gaaacctgtt gacgctgcgg tcatcactca cgcgcattca gaccacgccc gcatcgggtc
     9601 tcgatattat ctgacggaaa ccagcggaaa atccattctt caacagcgtt tggggccgga
     9661 cgcgcggatt gaaacattgc cttacggcga aagcgtctcc cgcaacgatg tgaaaatatc
     9721 gctgcacccg gcaggccata tactcggctc ggcgcaggtg cgcattgaaa gcggcggaga
     9781 agtctgcgtc gtcagcggag attataaatt ggaacacgac ggcatttgcc agccgtttga
     9841 gccggttcgc tgccacatgt tcgtgaccga atccactttc ggcctgccga tttatcattg
     9901 gcgtccgcaa gccgaaattt ttgcggagat aaatgactgg tggcgggaaa accgatcgcg
     9961 ggagcggacc agtgtcattt tctgttatgc cctgggcaag gcccaacgaa ttctttccgg
    10021 tttggatgcg agcatcggcc cgatttttct gcacggcgca atggaacgtt tcatgcccgc
    10081 ctatcatcaa gcgggcgtcc aattccccgc cacaacgagg gcgaccgccg aattggtaaa
    10141 aggagccggc ggtcgagcgg ttgttctggc cccgacgtct gctgacaact cgtcctggtt
    10201 gcgtaaattc ggcgcggttt ccacggcgtt tacttccggt tggatgcaaa ttcgcgggac
    10261 acgtcgtcgc cgctcattgg accgcggttt tgtgctctcg gatcatgcgg attggaccgg
    10321 tttgctggaa acgattgatg ccacaggggc agaaacggtt tgggcaacgc acggttac
//
LOCUS       DASZMM010000389         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1552000, whole genome shotgun sequence.
ACCESSION   DASZMM010000389 DASZMM010000000
VERSION     DASZMM010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552000"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VG754_09500"
     CDS             <1..254
                     /locus_tag="VG754_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19493.1"
                     /translation="GFFVTEMLTYPDRTDDICDYPAQYHNGGASFSFADGHSQVKTWT
                     TSLLLHPPAKNVIQQYPTPLAQFNADVFWLMDHATRIIP"
     gene            280..909
                     /locus_tag="VG754_09505"
     CDS             280..909
                     /locus_tag="VG754_09505"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HWF19494.1"
                     /translation="MNARQWALVSVLLGVFSIIPGIVFYGPLILGIPAIICGHVASIR
                     IHKSSSKQGKRAAIVGISLGYFGLVLTALLIILVEPMQARMRVKDEQRKCLFNLSQIA
                     LASKVWAADHNDTMPTNFLEMTNELSDPRILICPADPVHHEISAYTNEPFSIPRYIGH
                     KWNATNISYELLTSGMKKSALSNTVIVRCPVHGTELYGDGSVHLVKRPP"
     gene            912..1235
                     /gene="cutA"
                     /locus_tag="VG754_09510"
     CDS             912..1235
                     /gene="cutA"
                     /locus_tag="VG754_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HWF19495.1"
                     /translation="MNSPNFSIVLVTAPDLKVARRLAQAALRAKLIACANLVPKIESH
                     YWWQGKIESGNEVLLLMKTTRKHLAALEKLIVEKHPYDTPEFLVIPLTAGNTRYLKWI
                     DDSVK"
     gene            complement(1259..>3901)
                     /locus_tag="VG754_09515"
     CDS             complement(1259..>3901)
                     /locus_tag="VG754_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF19496.1"
                     /translation="DMRRELQSISPGSYPKFCSLELIRSKQFPDDWQGNAITCDFRAH
                     RVVRFAMDEKDSGYATREMPDLLRTTNVTFRPIDVKLGPDGALYIADWSNPIIQHGEV
                     DFRDPRRDHEHGRIWRITAKGRPLNKRVNFTKLSNRELLDQLLSPNAYDQQQSRRVLT
                     ERGRAVVPELAKWTGNQTNEFALLQALWMYESLDVVQPGLLGKSLNAEDGHIRAAATR
                     VVSYWHPRLNDPLDLLAQLVADKNPRVRVEAVRALAEIPSARSAELVLGALDKPKDPF
                     LDYAIWLSINDLAEPWVAAVKSGEWKSDGREKQLEFALRAVKTSYAGEVLSIELKKRT
                     IARDGSGPWIELIGKSGQEAEVVRLFNQAADGGFDDKAASQALKAVTQAARDRNVGKT
                     GDTEKLAVLFTNSNESIQENALSLAGAWQRKELIPQIGAIIKDKQTKSSVVKAGFDAL
                     RDIGGEGGSTQIAQLIKESDDKSVRLEAVLAYATLDMDKAKPYVISTLDDATTEKEAS
                     GIWHSVLAIKGASTAIAKALPTTGLPPVMAKAGLREAREGGRNETDLIVALTRGADLE
                     SQGLGLTDDDMKKISERAAKEGDATRGETIFRRKEIGCVNCHAIGGVGGKVGPDLTSI
                     GASAQPDYLVESVFYPNRKIKEGYHSVLVETKDGEELSGILVRENSEQLVLRDSTDKE
                     ITIAKDKIKGRTLGNSLMPSGLVDSLTTQQQLDLFRFLTELGKPGQFDASKGNIARLW
                     KLWPQTLDLSQFGDEKILNTKLSEDRWTTAFSLVDGHLLKSELDAAIQPVKGRDPAAI
                     FAGATFQVSGSGPFEFKLHGCSAQTPVWLDGKLVKLGLADDGDVWGQATPSAGTHTII
                     VKLNTAKLPDWIRLDVLGGTFLGN"
BASE COUNT          830 a         1127 c         1033 g          911 t
ORIGIN      
        1 acggtttttt tgtcactgaa atgctcactt atcccgatcg caccgatgac atctgcgatt
       61 acccggcgca atatcacaat ggcggcgcga gcttctcctt tgccgacggc cattcgcagg
      121 tgaagacatg gacgacatcg ctgcttcttc atccgccggc gaaaaatgtc attcagcaat
      181 atccaactcc gctggctcaa tttaacgctg acgttttctg gttgatggac cacgccaccc
      241 gaattattcc atgaaacagc agtcgaacgg tcaaaagaga tgaacgcgag gcaatgggcg
      301 ctagtcagtg tgctattggg agttttttcc ataatcccag gcatagtctt ctatggtcca
      361 ctcatcctcg gtatcccagc catcatctgc ggccatgttg cctcgattcg gatacacaaa
      421 tcgtcttcca agcaaggaaa acgggccgca atcgtgggaa tatctctggg gtatttcgga
      481 ctggtactta cggctttact gattattttg gttgagccta tgcaagctcg aatgcgggtt
      541 aaagatgaac aaaggaaatg tctctttaac ctaagccaaa ttgctttggc ttcaaaagta
      601 tgggctgccg accataacga tacgatgccc acaaatttcc tagaaatgac gaatgaactg
      661 tcagatcccc gcattctcat ctgtcctgca gatcccgttc accacgagat ttcggcttac
      721 acgaatgaac cattctcaat accgaggtat attggccaca agtggaatgc caccaatatc
      781 agctatgaac ttttaacttc tggaatgaag aaaagcgctc tttcaaatac agtcatcgtt
      841 cgttgtccag tccatggaac tgaattgtat ggcgatggct cggtgcacct cgtcaaacga
      901 cctccgtgac aatgaactct ccgaactttt ccatcgtgct tgtgaccgcg cctgacttga
      961 aagtcgcgcg gcgtcttgct caagcggctt tgcgcgcaaa gctgattgcc tgcgccaatc
     1021 tggtccccaa aatcgaatcg cattattggt ggcagggaaa aatcgaatct ggcaacgaag
     1081 tgttgctgct catgaaaacc acgcgcaagc atctggcggc gttggaaaaa ttgatcgtgg
     1141 aaaaacatcc ctacgacacg ccggaatttc tcgttatccc gctcactgcg ggcaacacgc
     1201 gttatctcaa atggattgat gattcggtga agtgatcgcg cccctctgcg gcgattgctc
     1261 aattccccaa aaacgtcccg cctaaaacat ccagcctgat ccagtcggga agttttgccg
     1321 tgttgagttt cacaatgata gtgtgcgttc cggcggatgg cgttgcctga ccccaaacgt
     1381 cgccgtcatc cgccaatcct aatttgacca atttgccatc gagccagacc ggagtttgcg
     1441 cggagcatcc gtgaagtttg aactcgaacg gccccgaacc ggacacttga aatgtcgcgc
     1501 ccgcgaaaat cgcggccggg tcgcgtcctt tgactggctg aatcgcggca tccaattcgc
     1561 ttttcagcag atgcccgtcc accagcgaaa aggcagtggt ccagcggtcc tcggaaagct
     1621 tggtgttcag gattttttca tcaccgaact ggctcaggtc gagcgtctgc ggccagagct
     1681 tccacaagcg cgcgatgttg cccttggaag cgtcgaactg gccgggtttg ccaagctcgg
     1741 tcagaaaacg gaacaggtcc aattgctgtt gcgtggtgag cgaatccaca aggccggacg
     1801 gcatgagcga attgcccaac gtgcggccct tgattttgtc tttcgcgatg gtgatttcct
     1861 tgtcggtgct gtcgcgcaag acaagctgct cgctgttttc gcgcacgaga atgccggaca
     1921 attcctcgcc atccttggtc tcgaccagca cggaatgata gccttccttg atcttgcggt
     1981 ttggataaaa aacggattcc accaggtaat ccggttgcgc gctcgcgccg atgctcgtca
     2041 aatcggggcc gaccttgccg ccgacgccgc cgatggcgtg gcaattgacg cagccgatct
     2101 ccttgcgacg gaaaatggtt tcaccgcgcg ttgcgtcacc ttccttggcg gcgcgttccg
     2161 agattttttt catgtcgtca tcggtaaggc cgagcccctg gctttccaag tccgcgccac
     2221 gcgtcaaggc gacgatcaaa tctgtctcgt tgcgtccgcc ttcgcgcgct tcgcgcaagc
     2281 ccgccttggc catgacgggc ggaagaccgg tcgtcggcag cgccttggca atcgccgtgg
     2341 aagcgccttt gatggccagc accgaatgcc agattcccga cgcttctttt tctgtggtcg
     2401 cgtcatcgag cgtcgaaatc acgtaaggtt tcgccttgtc catgtccagc gtggcataag
     2461 ccagcacagc ttcgagccgc accgatttgt cgtcgctctc cttgatcagt tgcgcgatct
     2521 gtgtcgaacc cccctcgccg ccgatgtccc gcaacgcgtc gaatcccgcc ttcaccacgg
     2581 acgatttggt ctgcttgtcc ttgatgatgg ccccaatctg cggaatcaat tccttccgtt
     2641 gccatgcgcc ggccaaactc aatgcatttt cctggatgct ctcgttggaa ttcgtaaaca
     2701 gcacggccaa tttttccgtg tcgccggtct tgcccacgtt ccggtcgcgg gcagcttgcg
     2761 taacggcttt caatgcctgc gacgccgcct tgtcgtcgaa accaccgtcc gcggcttgat
     2821 tgaagagacg aaccacttcg gcttcctggc cggatttgcc gatcagttca atccatgggc
     2881 cgctgccgtc gcgggcaatc gtacgtttct taagctcaat cgaaaggact tcgcccgcgt
     2941 atgaggtctt gaccgctctc aacgcaaact ccagttgctt ctcgcgcccg tcggatttcc
     3001 actcacccga tttcaccgcc gcaacccacg gttcggcaag gtcattgatg ctcaaccaaa
     3061 tcgcataatc cagaaacggg tccttcggtt tgtccaaggc gccaagcaca agttcagcag
     3121 aacgcgcgga tggaatttcc gccagcgcgc gcacggcttc gacacgcaca cgcgggtttt
     3181 tgtcggcgac aagttgcgcg agcaaatcca atgggtcgtt caagcgcgga tgccaataac
     3241 tcacgacacg agtcgccgca gcgcggatat ggccatcctc ggcgttgagc gatttgccga
     3301 gcaaaccggg ctgcaccaca tccagcgatt catacatcca cagcgcctgc agcagggcga
     3361 actcattggt ttgattgccg gtccattttg ccagctctgg aacaacggcc ctgccgcgct
     3421 ccgtcaaaac gcgccgtgat tgttgctggt cgtaggcatt gggtgaaaga agctggtcga
     3481 gcaattcgcg gttggaaagt ttggtgaaat tgacgcgctt gttcaaaggg cggcccttgg
     3541 cggtgatgcg ccagatgcgg ccatgctcat ggtcgcggcg cgggtcgcgg aaatccacct
     3601 cgccatgctg gatgatgggg ttcgaccagt cggcaatata cagcgcgccg tccggcccga
     3661 gcttcacgtc aatgggacgg aaagtgacgt tcgtggtgcg gagcaaatcc ggcatttcgc
     3721 gcgtggcata gccggaatct ttctcatcca tcgcaaagcg caccacgcga tgcgcgcgaa
     3781 aatcgcaggt gatggcgttg ccctgccagt catcgggaaa ttgcttgctg cggatgagtt
     3841 caaggctgca aaatttcgga taagaaccgg gactgatgct ttgcagttcg cggcgcatgt
     3901 c
//
LOCUS       DASZMM010000390         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3467598, whole genome shotgun sequence.
ACCESSION   DASZMM010000390 DASZMM010000000
VERSION     DASZMM010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3467598"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VG754_09520"
     CDS             <1..988
                     /locus_tag="VG754_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWF19497.1"
                     /translation="DILIYGFPGTEAEWYENPKGRDGDWQRHVIFDKVDNESPTFTDV
                     NGDGKPDIVCNSGGFFGYATADWEHPEKPWKFHPITPKGKWQRFTHGLGVGDVNGDGR
                     MDILASDGWWEQPASIEGDPVWKFHPFPFAPAVPGVPGSTGCSQMYVYDVNGDGKNDV
                     ICSLAAHGYGLAWYQQTNDDKGEITFIQHIILNPMTSKEKPAPDKYGVSFSQLHAVDL
                     IDMDGDGLKDIVTGKRFWAHGHNGPDPDSDGTAVLYWFKLVRGANGQVEFVPHLIDVD
                     SGIGTQVVAGDIQHKEFPDIVVGNKKGTFVFLHQAKKVGKAKWEKSQPKPVN"
     gene            1232..>3282
                     /locus_tag="VG754_09525"
     CDS             1232..>3282
                     /locus_tag="VG754_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HWF19498.1"
                     /translation="MKSSLKYFAILLTSFALTASVFAAQPDSIAPLGKDGHRLNLDFE
                     DGTLKDWTATGTAFDKQPVNGDTVSPRRGDMRSAHQGRYWIGTYEFGGDEPQGTLTSV
                     PFKVTQPWAAFLVAGGHFANTRVELVQADDDKVFFKTSGYDNETLRPVVVDLKAVEGK
                     EIFIRLIDQESGGWGHINFDDFKFYAARPELASELKAGPDNNEMPAADVIKFAGLPPE
                     QAAREMTLPPGFKATLFAGEPDVAQPIAFCIDDRGRLWVAEGFTYPQRAPEGHGKDRI
                     VVFEDTKGDGKFDKQTVFMEGLNLVSGLQVGFGGVWVGAAPYFMFIPMKDGDEPKPAG
                     PPQILLDGWNYNDTHETLNTFTWGPDGWLYGCHGVFNLSKVGKPGTPDKDRMTITCGV
                     WRYHPIKHTLEYFSEGTSNPWGVDFDEHGQCFIEACVIPHLWHMIQGAHYQRQGGNHV
                     NPYVFDDIKTIADHLHYAGNQGPHAANGRSGSMGGGHAHAGMMVYQGGSWPQQYWGQL
                     FMNNVHGFRMNMDIPERQGSGFVGHHGKDFILYNDSWSQVLDMESDQDGSVYLIDWYD
                     AQRCHSNDPKVVDKSNGRIFKIVYGDTKTTKVDLEKKSDLELVELQTSKNDWYARHAR
                     RILEERSTKSRLSNEADNRLEELLNLDDKPISYGKDYAPADVVELRALWTYHLTHFQI
                     DG"
BASE COUNT          782 a          863 c          927 g          710 t
ORIGIN      
        1 tgacatttta atctacggtt ttccgggaac cgaggctgag tggtatgaaa acccgaaagg
       61 ccgcgatggc gattggcagc ggcacgtcat tttcgacaag gtggacaacg aatcgccgac
      121 attcacggat gtcaacggcg atggcaagcc cgacattgtc tgcaattccg gcgggttctt
      181 cggttatgcg accgccgatt gggaacatcc ggaaaaaccg tggaagttcc atcccatcac
      241 acccaagggc aagtggcaac gcttcaccca cggcctcggt gtcggcgatg tgaatggcga
      301 cgggcgcatg gacattttgg cgagcgacgg ctggtgggaa caacccgctt cgatcgaagg
      361 cgatccggtc tggaaatttc atccgttccc tttcgcgccg gctgttcccg gtgtgccggg
      421 ctcgacgggc tgttcgcaaa tgtatgtcta tgacgtgaac ggcgacggca agaacgacgt
      481 catctgctcc ctggccgcgc acggttacgg cctcgcgtgg tatcagcaga ccaacgatga
      541 caagggcgaa atcaccttca tccagcacat catcctcaat ccgatgacca gcaaggaaaa
      601 acccgcgccg gacaaatacg gcgtcagttt ttcccaactg cacgcggtgg atttgattga
      661 catggatggc gacggtttga aggacatcgt cacgggcaaa cgtttttggg cgcacggcca
      721 caacgggcct gaccctgact cggatggcac ggcggtgctt tactggttca aattggtgcg
      781 aggcgcgaac ggccaggttg aatttgttcc gcacttgatt gatgtggatt caggcatcgg
      841 cacgcaagta gtcgctgggg acattcaaca caaggaattt ccggacattg tggtgggcaa
      901 caaaaaggga acgtttgtgt tcctgcacca agccaaaaag gtcggcaagg ccaaatggga
      961 aaaatcgcaa ccgaaaccgg tgaattgatt tcaactttgg atgggtgcgg agaatttcct
     1021 ttgaagcggg aaaatctcaa gatatttgcg caaaatctca tttgaaaagc gcctcggcgt
     1081 gataccctta agattcatca atgctgcatg tgtcatccgc gatcaagttt tgcccgcatc
     1141 aatccgcagc gcattttaga caacgactgc cgtttattgc cggtggattg ttgaatgatt
     1201 gcgccgttcg gacgtcttat ttttatctaa aatgaaatct tcactcaaat actttgccat
     1261 ccttctgact tccttcgctt tgacagcttc tgtcttcgcc gcgcaacctg actccatcgc
     1321 gccgctcggc aaggatggcc atcgcctcaa tctcgatttt gaggacggca cgctcaagga
     1381 ttggacggcc accggaaccg ccttcgacaa gcaaccggtc aatggcgaca ccgtttctcc
     1441 gcggcgcggc gacatgcgca gcgcgcatca aggacgctat tggatcggca cctacgaatt
     1501 cggcggggac gaaccgcaag gcacgctcac gtcggtcccc ttcaaggtca cacaaccatg
     1561 ggctgcgttt cttgttgccg gcgggcactt cgccaacaca cgcgtcgaac tcgtccaggc
     1621 cgatgatgac aaggtgttct tcaaaacgtc cggttacgac aacgaaacgc tgcgtccagt
     1681 cgtcgtggat ctgaaggcgg tcgagggcaa ggaaattttc atccgcttga ttgaccagga
     1741 aagcggcggt tggggccaca tcaattttga cgatttcaag ttctacgcgg cgcgtcccga
     1801 attggccagc gaactcaagg ccggtcccga caacaacgaa atgccggcgg cagacgtcat
     1861 caaatttgcc gggcttcctc cggaacaagc agccagggaa atgaccttgc cgcccggttt
     1921 caaggcgacg cttttcgccg gcgaaccgga cgttgcgcag ccgattgcct tttgcattga
     1981 tgatcgcgga cggctttggg tcgccgaagg cttcacttat ccacaacgcg cgccggaagg
     2041 ccatggcaag gaccgcatcg tggtgttcga agacacgaaa ggcgacggca agttcgacaa
     2101 gcaaacggtg ttcatggaag ggttgaactt ggtgagcgga ttgcaggtcg gtttcggcgg
     2161 tgtgtgggtc ggcgcggcgc cgtatttcat gttcatcccg atgaaggacg gcgacgaacc
     2221 gaaacccgcc ggtccgccgc aaatcttgct cgacggctgg aattacaacg acacgcatga
     2281 aacattgaac actttcacat ggggaccgga cggctggctt tacggatgcc acggagtttt
     2341 caacttatcg aaagtcggca agccgggcac gccggacaag gaccgcatga cgattacctg
     2401 cggtgtctgg cgttatcatc ccatcaagca cacactcgaa tatttttccg aaggcacgag
     2461 caatccgtgg ggcgtggatt ttgacgagca tgggcaatgt ttcatcgagg cgtgtgtgat
     2521 tccgcatttg tggcacatga tccaaggcgc gcattatcaa cgccagggcg gcaaccatgt
     2581 gaacccgtac gttttcgacg acatcaagac cattgccgac catttgcatt acgctggcaa
     2641 tcaaggtccg cacgcggcga acgggcgttc cggctcaatg ggtggcggcc atgcgcacgc
     2701 gggcatgatg gtttatcaag gcggaagctg gccgcagcaa tattgggggc aacttttcat
     2761 gaacaacgtc catggctttc gcatgaacat ggacatcccc gagcgccagg gcagcggatt
     2821 tgtcggccat cacggaaaag atttcattct ctacaatgat tcgtggtcgc aggtgctcga
     2881 catggaatcc gaccaggacg gctcggtcta tctgattgat tggtacgatg cgcaacgctg
     2941 ccatagcaat gaccccaagg tcgttgacaa atcgaacggt cgcattttca aaattgttta
     3001 cggcgacaca aaaacgacca aggtggattt ggagaagaag agtgatttgg aattggttga
     3061 attgcaaacg agcaagaatg attggtacgc gcgccatgct cggagaattt tggaggaaag
     3121 aagtacaaaa tcgcgtctct caaacgaagc agataaccgg ctagaggaat tgcttaatct
     3181 tgatgacaag ccaataagtt acggaaaaga ttatgccccc gcagatgtcg tcgaacttcg
     3241 cgccctttgg acctatcatt tgacgcattt tcagatagat gg
//
LOCUS       DASZMM010000391         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2633635, whole genome shotgun sequence.
ACCESSION   DASZMM010000391 DASZMM010000000
VERSION     DASZMM010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633635"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VG754_09530"
     CDS             complement(<1..1646)
                     /locus_tag="VG754_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19499.1"
                     /translation="MKRRHNNSKPVHPQKDGGQPHKPSPSNTTRRLSPARKWLFRLLA
                     LVLIPLLIFGGLETGLRMAGYGRTLHFFKKINIDGRDYLVNDDRFGLKFFPPEMIRSP
                     SALRMEPQKPPGVFRIFIFGESAAMGDPEPAYGPGRYLETLLRERYPGQRFEIVNFGI
                     TAINSHVILPIARECAPRQGDLWVIYMGNNEMVGPFGAATVFGAQAPPALLVRATLAI
                     QETRTGQLLAALGRKLKGKPGGTHAWGGMQMFTENKIPPDDRRRETVHRNFEKNLRDI
                     VRTGLGSGAKILLSTVAVNLKDCPPFASQTLASFTAANYEQAMANANAAEAQGNFALA
                     ISPLEQAVKLAPQSAEANYKLGRSFLRLGDATNARTALARACDFDTLPFRADSRINAL
                     IADAGRRYGGERLRCLDAAAALSTNDSLCGDEIFYEHVHFNFDGGYRLGRAWAAQIEA
                     MLPPTMKSRSDWASQETCERRLGLTDWNRVAIIDSVIKRLYEPPLSTQANNTERIAAL
                     RAQAEELRQRMSVPDATNAAREIYLEALRLSPDDFFLREN"
     gene            complement(1711..2598)
                     /locus_tag="VG754_09535"
     CDS             complement(1711..2598)
                     /locus_tag="VG754_09535"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF19500.1"
                     /translation="MKARNATSASNGFTLIELLVVIAIIAILAGLLLPALARAKQKAM
                     ATACMNNGKHITLAWHMYADDNNDHLAVNADGSDSDTGSSTGAGDPSWVGPIYLNWNV
                     DPNFTNTTLLTDPKYSSLGQYVANNVDIYRCPADSYLSPAQKAAGWSHRSRSIAMDAA
                     IGPGNPKANQPGNKAPSSLPYLNPFFYAITMGDLVNPGPSQSWLFLDEHADSIDDGIL
                     YTSPSETSGNGTFTELPSSDHNGACGISFADGHSEIHKWQNPQTVLPVIYNQSARQRI
                     SVVQDPDLAYLARATPKAN"
     gene            complement(2701..>4189)
                     /locus_tag="VG754_09540"
     CDS             complement(2701..>4189)
                     /locus_tag="VG754_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CBM20 domain-containing protein"
                     /protein_id="HWF19501.1"
                     /translation="QVSGTFNSHTNGFYLSPGSNPNIYTATYNSSDALGTPETFAYTI
                     IHGAGGSTPESTDARTFTLTNTQTLPGIYFSSYAPATNATVPLSFSVDMGVQIAAGSF
                     NPGAGDQVFVYGDMNENAALAWGGSVALTPSGTNTNVYVGTFGDGNYAGTPCQYKFVR
                     YSTALGQNVYESRANRTYNTPSSATNFPTVYFNDSASVTVVSFTVDMAAQTQFGKFTP
                     GSDTIAVAGSFTGWAGGAVTCTNDVNTNLYSISIPIYDTTGTTEQYKFITYGPGISGT
                     DYESPSSGGNRSFVLPSSSTNLPVVAFSDQYLSDYLLTDTTVIFTVNMSNAIQFPSGT
                     PFNSGSDSVYVSGDFLGWPSWSASLPQLSPEGSSLLYTYTNVFAAGSPVVINYKYSID
                     GSDDEAPGGANHIRYIRGYGTYQMPVDTFGTQLVEQSFGQLVAGAPSSGHIPISWLGR
                     PGVHLQVSSSVKGPWVDHAETDAMSFTNWPLGGSSLFFRLVHPEQ"
BASE COUNT          934 a         1128 c         1212 g          915 t
ORIGIN      
        1 caattttcac gcagaaaaaa atcgtccggc gaaaggcgca acgcttcgag gtaaatttcc
       61 cgcgcagcgt ttgtcgcatc tggcacggac atgcgttggc gcaattcctc cgcttgcgcg
      121 cgaagggccg caatcctttc cgtgttattt gcctgcgtgc tcaaaggcgg ctcatacaaa
      181 cgcttgatca cggaatcaat gattgccacg cggttccaat ccgtcaggcc caatcgtcgc
      241 tcacacgttt cttgcgaagc ccaatcggaa cggcttttca tcgtcggcgg cagcatggct
      301 tcaatttgcg ccgcccatgc gcgcccaagg cgataaccgc cgtcgaaatt gaaatgcaca
      361 tgctcgtaaa aaatttcgtc tccgcaaagc gagtcattcg tggacaacgc ggcagcggca
      421 tccagacagc gcaatcgctc gccaccgtat cgacggcccg catcggcgat gagcgcgttg
      481 atgcgggaat cggcgcgaaa aggcagagtg tcgaaatcgc acgcacgcgc cagagcggta
      541 cgcgcatttg tggcatctcc aagacgcaag aaagagcggc ccagcttgta attcgcttca
      601 gcggattgcg gggcaagttt cacggcctgt tccaacggcg aaatcgcgag cgcgaaattg
      661 ccctgagctt cggcggcatt ggcgtttgcc atggcctgtt cataattggc agcggtgaag
      721 ctggcgaggg tctgcgaggc aaaaggagga caatccttca agttgacggc gacggtgctc
      781 aacaaaatct ttgcgccaga gccaagcccg gtccgaacga tgtcacgcag gtttttttca
      841 aaattgcgat gcaccgtttc gcggcggcgg tcatccggtg gaattttatt ctcggtgaac
      901 atctgcatcc cgccccaggc gtgtgtgccg cccggtttgc ctttgagttt gcgcccaagc
      961 gccgcgagca attgaccggt gcgcgtttcc tggatggcga gagttgcgcg caccaacaac
     1021 gcgggcggcg cttgcgcgcc aaaaacagtg gcagcgccga acggcccgac catttcgttg
     1081 ttgcccatgt agatgaccca taaatcaccc tggcgcggtg cgcattcgcg cgcgatcggc
     1141 aggatgacgt gcgaattgat ggccgtgatg ccaaaattta cgatctcaaa acgctggccg
     1201 ggataacgct cgcgaagaag cgtttcaaga taacgcccgg ggccgtaagc gggttccgga
     1261 tcgcccatcg cggcggattc gccgaagata aaaattcgaa acacgcccgg aggtttttgc
     1321 ggttccatgc ggagcgcgga aggcgaacga atcatttccg gcggaaaaaa tttgaggcca
     1381 aaccggtcgt cattgacgag atagtcgcgg ccatcaatgt tgattttttt gaagaaatga
     1441 agcgtgcgtc catacccggc catacgcaga ccggtttcaa gtcctccgaa gatcaacaac
     1501 ggaatgagca ccaatgccag cagacgaaac aaccacttgc gggctggtga taaacgacgg
     1561 gtggtgttgg acggtgaagg tttatgcggc tgaccgccat ctttttgcgg atgaaccggc
     1621 tttgagttgt tatgccgccg tttcacgttt tgcaaccgaa cgatccggaa cgtgaatctt
     1681 ttttattcgc gcgcgacctg ccaccgacgg tcaattcgcc ttcggcgttg cgcgcgcgag
     1741 gtaagcgagg tccgggtcct ggacaacgga aattctctgc cgcgcggact gattataaat
     1801 caccggcagc acggtctggg gattctgcca cttatggatt tccgaatggc cgtcagcgaa
     1861 gctgatgccg caggcgccat tatggtcgct ggaaggcaac tcggtgaatg tcccgttgcc
     1921 tgaagtttca cttgggctgg tgtaaaggat gccgtcatct atgctgtcag cgtgctcgtc
     1981 caggaacagc cagctctggc ttggaccagg attgaccagg tctcccatgg taatcgcata
     2041 aaaaaacgga ttcagataag gaagagaact gggcgcttta ttaccgggct ggttcgcttt
     2101 cggattgcca ggcccgatgg cggcatccat ggcgatgctg cgactccgat ggctccaacc
     2161 agccgctttt tgcgccgggc ttagataact atcggcaggg cagcggtaaa tatccacgtt
     2221 gtttgccacg tattgtccca agctggagta cttcggatca gtgagcaacg tggtatttgt
     2281 aaaattagga tccacgttcc agttcaggta aatcggcccg acccacgatg ggtcgccggc
     2341 gccggtacta ctacccgtgt cggaatccga gccatccgca ttgaccgcca aatgatcgtt
     2401 gttgtcatcc gcatacatgt gccaggcaag ggtgatgtgt tttccattgt tcatgcacgc
     2461 cgtcgccatt gctttttgtt tggccctggc caaggcgggc aacagcagac ccgcgagaat
     2521 ggcgatgatg gcgatcacga ccaacaactc aatcaaggtg aaaccatttg aagcggatgt
     2581 tgcatttcga gctttcatac gacttgggat gcgagtgatt tcaaacggtt tcatgacttt
     2641 ggccggaacg ccaaagacgt tccggccgga tgggagttgc tgttagccat cagtatagca
     2701 tcattgttcc gggtgcacga ggcggaagaa caaactcgac ccgcccaacg gccagttcgt
     2761 gaaactcata gcgtcggttt ccgcatggtc cacccacggc cctttaacgc tgctgctgac
     2821 ttgcagatga actcccggac gtcctagcca gctaatcggg atatggccac ttgaaggcgc
     2881 cccggccacc aactggccga atgattgttc gacaagctga gtgccgaacg tgtccaccgg
     2941 catctgatag gtcccgtaac cccggatata acggatgtgg ttcgccccgc ccggcgcttc
     3001 gtcgtcgctg ccgtcaatgc tgtatttgta attgatgacc accgggctgc cggcggcgaa
     3061 cacattcgtg taagtgtaaa gaaggctgga accttccggc gacaattgcg gcaaggaagc
     3121 cgaccatgac ggccagccca ggaagtcgcc actgacataa acactgtcgc tgcccgaatt
     3181 gaacggcgtg ccgctcggaa actgaatggc attgctcatg ttgacagtga agatgaccgt
     3241 ggtatcggtc agcaaataat cgctgagata ttggtcgctg aacgcgacca ccggcagatt
     3301 ggtgctgctg gaaggcagaa cgaaagagcg attgccgccg cttgaggggc tttcatagtc
     3361 ggtgccgctg atgcccggac cataagtgat gaacttatac tgctccgtgg tgccggtcgt
     3421 atcatagatg gggatggaaa tgctgtagag gttggtgttg acatcattgg tgcaggtcac
     3481 cgctccgccc gcccaacccg tgaaactgcc tgccacggcg atggtgtccg aaccgggagt
     3541 gaatttcccg aactgggtct gagccgccat atcaaccgtg aagctcacaa ccgtgacgct
     3601 ggcagagtcg ttgaaataaa ccgttgggaa gttcgtggcg ctggatggcg tgttgtaagt
     3661 gcgattggcc cggctttcgt aaacgttttg accgagcgca gtggaataac gaacaaattt
     3721 atattggcag ggcgttccgg cataatttcc atctccgaat gtgccgacat acacgttcgt
     3781 gttggttcca ctcggcgtca aagccacgct gccaccccat gcgagcgcgg cattctcatt
     3841 catgtcgcca tacacgaaga cctggtcgcc agcgccggga ttgaagcttc ccgcggcgat
     3901 ttgcactccc atgtccacgc tgaaactaag aggcacggtg gcattggttg caggcgcgta
     3961 agagctgaaa taaatccccg gcaaagtttg tgtattcgtc aacgtgaacg tgcgtgcgtc
     4021 ggtggattca ggagtgctcc cgccagcgcc atgaatgatg gtataagcga aggtttcagg
     4081 cgttcccaat gcatccgaag agttgtaagt cgccgtatag atgttgggat tggagccagg
     4141 gctcaaataa aatccattgg tatgactatt gaaggtgccg ctgacctgc
//
LOCUS       DASZMM010000392         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1554518, whole genome shotgun sequence.
ACCESSION   DASZMM010000392 DASZMM010000000
VERSION     DASZMM010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554518"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2258)
                     /locus_tag="VG754_09545"
     CDS             complement(<1..2258)
                     /locus_tag="VG754_09545"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HWF19502.1"
                     /translation="MNDQERRELEQLKSRQAELLHDLAELGRKLEDFEHRMAAPPQPQ
                     APPILVPKPEPLVQPTASPSVAEAPALVATPMEAPTPPPVAAPPPIQPPPPIAPPPIP
                     PREFKLPPIRPIPATIPVMAQAKIAEAPSSAPVVPPAGEKKSSLEMRLGTFWLVRIGI
                     VMLLTSLVFFGTYAYQNFIPKLGASGKVALLYLASASLLGIGSWLQRKQESLKNYAQV
                     LQAGGLAAVYFTTYAAHHFPNLEIIHNAVLDGALLLGWAGFIIWLADRKKSEVLALFA
                     VLLAYYTSVITHAGLFTLFSNLVLTVAAVFFLVRNRWATLSFASLVCTYVSYAFWRFA
                     FGFHAGEWHWTTPLEGLWMGNYFLMGYWLLFSAAVFLSKHETFNGERRASFLSLNNAA
                     FFSLFLLTMLQVHQGGFWKFSLIYGAVLIGLSALAWRMLAKDRLSVNAYLTQGLLLVT
                     LGFLTRLAGLKLSLVLAVESVILLSLGQLLKSRVMQAGSIVTAAMAAGWTLATLEPFD
                     RDGLLAAIAVGAMLLWNAFSLRRNTTYERSITHPRTLIYTALALAVWIYATGQNCKPE
                     WCGLAIACEMVAFLLAAKPLGNQPLRFGAFACAGIVLGWEIFTLDEQLMSVEFSQRTG
                     LLQGVSTGALMIFSSIWEQRSVRRDEKQTFVPSVIFFSAVGALVWLMVTGVFTPREHL
                     ALAFALEAVLFTLMSYPLRLRELALYGQAVLLLSQALWLYDSGDKAKAHPWWNPVLII
                     ACTLGLAAWCQR"
     gene            2463..2963
                     /gene="nusB"
                     /locus_tag="VG754_09550"
     CDS             2463..2963
                     /gene="nusB"
                     /locus_tag="VG754_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWF19503.1"
                     /translation="MGKRREARERAIQFLFQHDLNPPEKLDEALNHFWESQRVAAIAQ
                     EKGRAKWGQKVELPAQTAEEGEIRLFAEPLIRGTLDHRAEADDVIKKYVQNWELHRIA
                     AVDRNILRLAIYEMLHREDIPPVVSINEAVDIAKKFSTHDSGKFVNGILDKVKGDLMR
                     PARNVK"
     gene            2965..>3720
                     /locus_tag="VG754_09555"
     CDS             2965..>3720
                     /locus_tag="VG754_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWF19504.1"
                     /translation="MSFADLHLHTNFSDGTYTPEELVSHGKRCGLAALALTDHDTVEG
                     CARMSVACEAVGIEFISGTELTAELNGIELHLLAYCVDLDNQKLLTEVGRFQVVRQNR
                     IREMVARLNRLDIPLQADAVFAIANCRSPGRPHVARALVQAGLCSSLDEAFERFLKMN
                     RPAWVPKSKMSAVDAITMVHEAGGVAVMAHPGLNRTDDIIPEMVESGLDGIECFHSKH
                     STAVAQHYLQMADQFHLLVTGGSDCHGMNKGKPL"
BASE COUNT          851 a         1019 c         1138 g          712 t
ORIGIN      
        1 tgtctctggc accacgcggc gaggccgagt gtgcaggcga tgatgagcac cgggttccac
       61 catggatggg ccttggcctt gtcgccgctg tcgtaaagcc aaagcgcctg cgaaagcaaa
      121 agaacagcct gtccatataa agccagttcg cgcaatcgca gcggatagga catcaaggtg
      181 aacaacaccg cttcgagcgc aaatgccagg gcaagatgtt cgcgcggcgt gaagaccccg
      241 gtgaccatca gccaaacgag cgcacccacc gctgaaaaaa agatgacgct tggaacgaag
      301 gtttgctttt catcacgccg gacggagcgt tgttcccaaa tgctgctgaa aatcatcagt
      361 gcgccggtgc tcactccctg gagcaatccg gtcctctgcg aaaattccac cgacattaat
      421 tgctcgtcga gcgtgaaaat ttcccatccc aaaacgatgc ccgcgcacgc aaaagcgcca
      481 aaacgcaacg gctggttgcc caaaggcttc gcggcgagca ggaacgcgac catttcgcag
      541 gcgatggcca gcccgcacca ctccggtttg caattttgcc ccgtggcata aatccagacg
      601 gccaacgcca gcgcggtgta aatcagcgtc ctcggatgag tgatgcttcg ctcgtaagtg
      661 gtgttgcggc ggagcgagaa cgcgttccac aacagcatcg cgccgaccgc aatggccgcg
      721 agaaggccgt cgcggtcgaa cggttcgagg gttgccaacg tccagcctgc tgccatggcg
      781 gcagtgacga tcgagcccgc ctgcatgacg cggcttttca aaagttggcc gagcgaaagc
      841 aggatgacgc tctccaccgc cagcacgagg ctgagcttga gcccggcaag acgggtgagg
      901 aaaccgagcg ttaccagcaa caaaccttgc gtgaggtagg cgttgaccga aagcctgtcc
      961 ttggccagca tccgccacgc gagcgcagaa agtccgatca acaccgcgcc gtaaatgagc
     1021 gagaatttcc aaaagccgcc ctggtggacc tgaagcatcg tgagcaggaa caacgagaaa
     1081 aacgcggcat tgttcaggct cagaaatgaa gcgcgccgtt cgccattgaa tgtttcgtgc
     1141 ttggacaaaa agacggcggc ggaaaacaac agccaataac ccatcaagaa ataatttccc
     1201 atccacaatc cttcaagggg agtggtccag tgccattcac cggcgtggaa cccgaaggca
     1261 aaccgccaga aggcgtagct cacgtaagtg caaacgaggc ttgcaaacga aagtgtcgcc
     1321 caacgattgc gcaccaggaa gaaaaccgcc gcgacggtca acacgaggtt cgaaaaaagc
     1381 gtgaacaacc ccgcgtgggt gatgacggaa gtataatacg cgagcagcac ggcaaagagg
     1441 gccagcacct cggatttctt gcggtctgcc agccagatga tgaatccggc ccagcccagc
     1501 agcagcgcgc cgtccaacac ggcgttgtgg atgatttcga gatttggaaa atgatgcgcg
     1561 gcgtaggtgg tgaaatagac cgcggccaat ccgcccgctt gcaggacttg cgcgtaattt
     1621 ttgagcgatt cctgcttgcg ttgcagccac gagccgatgc ccagcaaact tgcgctcgcc
     1681 aggtagagca acgccacttt gccggacgcg cccaatttag ggataaaatt ctggtaagcg
     1741 taagtgccga agaatacgag gctcgtcagc aacatcacga ttccgatgcg cacgagccag
     1801 aaagtcccga ggcgcatttc caaagaactt ttcttttcgc cggcaggcgg aactactggc
     1861 gctgacgacg gcgcttcggc aatcttcgcc tgggccatca cgggaattgt cgccgggatt
     1921 gggcgtattg gcgggagctt gaattctctg ggtggaatgg gaggtggcgc aatcggtgga
     1981 ggcggctgga tcgggggcgg agcggcaacc ggaggaggcg ttggcgcctc catcggggtt
     2041 gccacgagcg cgggtgcttc cgcaacggag ggcgaagctg tgggttggac cagcggttcc
     2101 ggtttgggaa caaggatggg aggcgcttgc ggttgcggcg gagcggccat ccgatgctcg
     2161 aagtcttcca atttgcgccc gagttccgcc aaatcatgca gcaattccgc ctggcgcgat
     2221 ttcaactgct ccagttctct gcgctcctgg tcgttcatgg cttctcaggc ggctgttggc
     2281 gaaatcctct atcttggttt agtgtcatga gggtatgtta gccgggggcg tgatggaagt
     2341 gttaatcgaa aatctttcgc cggccattga ttcagcagat gttcgacgcc agccggagtt
     2401 tgcgggagca agggccaaac attttcatat cgctgccggg atttcttggt catcatggtg
     2461 ttatgggcaa gcgaagagag gcgcgcgaac gcgccatcca atttttgttt cagcacgatt
     2521 tgaatccgcc ggaaaagctg gatgaggcgc tcaatcattt ctgggaatcg caacgcgtcg
     2581 cggcgattgc ccaggagaag ggccgcgcca agtggggcca aaaggtcgaa ttgcccgcgc
     2641 agacagccga ggaaggggag atccgtttgt tcgcggagcc gttgatccgc ggcacgctcg
     2701 accatcgcgc tgaagcggat gacgtcatca aaaaatatgt ccagaactgg gagttgcacc
     2761 gcatcgccgc cgtggaccgc aacattctgc gccttgccat ttatgaaatg ctccatcgcg
     2821 aggacatccc gcccgtggtg agcatcaacg aggcggtcga tatcgccaaa aaattttcca
     2881 cgcacgacag cggcaagttt gtcaacggca tcctcgacaa ggtgaagggc gacttgatgc
     2941 gtccggcgag aaatgtgaaa tgaaatgagt ttcgccgatc ttcatctgca cacgaatttc
     3001 tccgacggca cttacacgcc ggaggaattg gtgtcgcacg gaaaacgttg cggcctggcg
     3061 gcgcttgcgt tgaccgacca tgacacggtg gaaggttgcg cgcggatgtc ggtggcgtgc
     3121 gaggcggtgg gaatcgaatt catctcggga acggaattga ccgcggaact caacggcatc
     3181 gaacttcatc tgctggccta ttgcgttgat ctcgacaacc aaaaattgct gaccgaagtc
     3241 ggccgctttc aagtcgtgcg gcaaaatcgc atccgcgaaa tggtggcgcg tttgaaccgg
     3301 ctcgacattc cgttgcaggc ggacgcggtg tttgcgatcg ccaattgccg ttcgccgggg
     3361 cgtccgcacg ttgcgcgcgc gctggtgcaa gcgggcttgt gcagcagttt ggatgaggca
     3421 ttcgagcgtt ttctcaagat gaaccggccc gcgtgggtgc ccaaatcgaa gatgtccgcc
     3481 gtcgatgcca tcacgatggt gcatgaagcc ggcggcgtgg ctgtgatggc gcatccggga
     3541 ttgaaccgca ccgacgacat cattcccgag atggtggaga gcggtttgga tggcatcgaa
     3601 tgttttcatt ccaaacattc caccgcggtg gcgcagcatt atctgcaaat ggccgatcaa
     3661 tttcatttgc tggtgaccgg cggttcggat tgtcacggca tgaacaaggg caagccgctc
//
LOCUS       DASZMM010000393         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3719143, whole genome shotgun sequence.
ACCESSION   DASZMM010000393 DASZMM010000000
VERSION     DASZMM010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3719143"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..799
                     /locus_tag="VG754_09560"
     CDS             293..799
                     /locus_tag="VG754_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF19505.1"
                     /translation="MKEKNVRDIGSHCVVALRPEKAEDEALLAEIYAGTREDELNLTN
                     WDAATRAAFLDMQFKAMLKGYRSMYAQAQFSIILSDGKAVGRIVVDRAPGEIRVVDVA
                     LLPAHCGHGIGTKLMRDVMDEAAGQGKPVRLTVFHGTRAIAFYQRLGFRIISKPDIYL
                     QMEWTPPG"
     gene            complement(825..1163)
                     /locus_tag="VG754_09565"
     CDS             complement(825..1163)
                     /locus_tag="VG754_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19506.1"
                     /translation="MNSTPLESLTQAKFAECLNTGFQVWINSTETMELRLAEVTSGLM
                     AASNGQRGGQFESFSLMFHGPNSRFLPQKTYTFEHPRLGKFELFIVPVGRDGDLFKYQ
                     AVFNRRIQSA"
     gene            1469..2581
                     /locus_tag="VG754_09570"
     CDS             1469..2581
                     /locus_tag="VG754_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWF19507.1"
                     /translation="MTANFSFPTKIRFGAGTLRELPGHLAALGVSKPLIVTDPGLIPT
                     DAFKSLEKISGKKWSVFSGVHPNPAMEDVEAATAAYQDGKCDSVIAFGGGSALDVGKV
                     IRICIKRPDKPLIPFDFKADWSGLVPCVTIPTTAGTGSEAGRSSVIIKDGRKQVIFHP
                     ALLASLVILDPELTLGLPPKLTAATGADALIHCIESFTSPVFHPMCDGIALEGIHLIS
                     QSLARAVKDGGDLEARGKMLVAAMMGAVAFQKDLGAIHSLAHPLSTFCGLHHGTANAL
                     CAPFVMKFNAERVPGIYRRVGIALGLNNPDDAATIEHVSGMFREIGLAPGLRAYGVKE
                     SLLDAMSDQAFEDTCHQTNPVPVTRNDLRALYQLAF"
     gene            2585..>3491
                     /locus_tag="VG754_09575"
     CDS             2585..>3491
                     /locus_tag="VG754_09575"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWF19508.1"
                     /translation="MQYPAKLFINGNSCAASDQRTLPLINPATEETLVEVASASERDV
                     ETAIIGAQKTYEQSWRDHTPGKRAEVMFQIARVLREHAEELSQLESLSIGKPISDARD
                     EIAMGARTFEYYAGAISKFYGQTIPVARGGFDFTLRQPMGVVAAIVPWNFPFPIATWK
                     VAPALAAGNCVVLKPARQSPLTALRLGELAAQAGLPPGALQVLAGSGEQMGDALVKHP
                     LIRKISFTGSTSVGSHIMQLAARDIKRVSLELGGKSPNIIFADCDLEKAAATSPMSVF
                     ANTGQDCCARSRIFVERSVYEKFVEL"
BASE COUNT          771 a          961 c          999 g          760 t
ORIGIN      
        1 caagtcagtt tccccaaaag agacgaacgc ttcgcgttgt gtggtccccg aggagagggg
       61 gaaccatttg gccgctgctc gataaatcga acgctctcgg tttgcaacca cgacggcatg
      121 cgttgctccc tctccccaag ggagagggct ggggtgaggg ggaggaaacg ttcaattact
      181 ccgtcgtgca ggaaactgag atgcgcccgc ttgaaccata aagaaaagga cggtcttgat
      241 tttacagtca ttcccggcta cggcttgagc gctcttttca cgatttatcc gcatgaaaga
      301 aaaaaatgtt cgcgacattg gcagccattg cgttgtcgct ttgcgcccgg agaaggcgga
      361 agacgaggcg ttgttggcgg agatttatgc cggcacgcga gaggatgaat tgaacctgac
      421 gaattgggac gctgcaaccc gcgccgcatt cctcgacatg cagttcaagg caatgctcaa
      481 aggctaccgc tccatgtacg cgcaggcgca attctccatc atcctctcgg acggaaaagc
      541 tgtgggacga atcgtggtgg accgcgctcc cggggaaatt cgcgtggtgg atgtggcgct
      601 tttgccggcg cattgcggcc atggcatcgg aacgaagttg atgcgggacg tgatggacga
      661 agcggcgggg cagggaaaac cggtgcgttt gacggttttt cacggcacgc gcgcgatagc
      721 cttttatcaa cggctgggat ttcgcatcat ttccaagccc gatatatatc tccaaatgga
      781 atggactccg ccgggttgag tctgccgcaa cgattggggc cggcttacgc agattgaatg
      841 cggcgattga atacggcttg atacttgaac aggtcaccgt cacggccgac agggacaatg
      901 aaaagctcga atttgcccaa acgggggtgc tcgaatgtat atgttttctg cggaagaaag
      961 cggctgttgg gaccgtggaa catcagggaa aaactttcaa actgtccgcc acgttgtcca
     1021 tttgacgcgg ccatcaaacc cgacgtcact tcggccaacc gcaactccat cgtctccgtg
     1081 gaattaatcc acacctgaaa cccggtattc aaacactcgg caaattttgc ttgcgtaagg
     1141 ctttccagcg gcgtggaatt catttagacg aagtgtatcg cgggacgtgc gacaggtcaa
     1201 actctgcgaa atcagattga gcaaaattcc ctgattacag gcgacagttc ggccaagcct
     1261 ccgccgggca aaggaaattg caagcagtaa cggcggctta ttttgccgaa gaattcaacg
     1321 tgccgcaaaa atttgacgcg aggccaaccg agagtttgct tgtttccagc gcggttgaca
     1381 taaccttcga gcccggcttt attgacgacg ggtcttacga gccgtcatgt gaagaatatt
     1441 gcaagatgtc gctccatgat ttcataaaat gacagcaaat ttctccttcc ccacgaaaat
     1501 ccgcttcggc gccggcacgc tccgggaatt gcccggtcac ctcgccgcgc tcggcgtgag
     1561 caaaccgttg attgtcacgg atcccggact gattcccacc gatgctttca agtcgttgga
     1621 aaaaatttcc ggcaaaaaat ggtcggtttt ttccggcgtc catcccaacc cagccatgga
     1681 agatgtggaa gccgccactg ccgcttacca ggacggaaaa tgcgattccg tcatcgcctt
     1741 cggcggcgga agcgcgctcg atgtcggcaa ggtgattcgc atctgcatca aaaggccgga
     1801 caagccgctc attccgtttg attttaaagc tgattggagc gggcttgttc cgtgcgtcac
     1861 cattcccaca acggccggca ccggcagcga ggcaggccgc agctcggtca tcatcaagga
     1921 cggtcgcaaa caggtcatct tccatcctgc gttactggcg agtctggtga ttctggaccc
     1981 ggaattgacc ctcggtctgc cgccaaaatt gaccgccgca accggcgcgg acgccttgat
     2041 tcattgcatc gaatcgttca cttcaccggt ctttcacccg atgtgcgacg gcatcgcgtt
     2101 ggaaggcatt catttgatca gtcaatccct ggcgcgcgcg gtgaaggatg gaggcgacct
     2161 cgaagcgcgc ggcaagatgc tggttgcggc gatgatgggc gcggtcgcat ttcaaaaaga
     2221 ccttggggca atccattcgc tggcgcatcc gctttccaca ttttgcgggc tgcaccatgg
     2281 caccgcgaac gccctttgcg cgccgttcgt catgaaattc aacgccgagc gcgttccggg
     2341 aatctatcgt cgcgtgggca ttgcgctcgg cttgaacaat cccgacgatg cggccaccat
     2401 cgaacatgtc agtggaatgt tccgggaaat cggccttgcg cccggcttgc gcgcgtatgg
     2461 cgtgaaggaa tcgttactgg acgccatgag cgaccaggca tttgaggaca cctgccatca
     2521 aaccaatccc gtgcccgtga cccggaacga tttgcgcgct ctttaccagc ttgcgttttg
     2581 acccatgcaa tatccggcca aactttttat caacggcaac tcctgcgccg cttcggacca
     2641 acggacgctg ccgctcatca atcccgccac tgaagaaact ctcgtcgagg tcgcttcagc
     2701 gagcgaacgc gatgtggaaa ccgccatcat cggcgcgcaa aaaacctacg agcaaagctg
     2761 gcgcgatcac acacccggca aacgcgcgga agtgatgttt caaatcgcgc gggtgttgcg
     2821 cgagcacgcg gaggaattat cgcaattgga aagcctttcg atcggcaaac cgatttccga
     2881 tgcgcgcgat gaaattgcga tgggcgcgcg cacgtttgaa tactacgccg gtgcgatttc
     2941 aaaattttac ggtcaaacga ttccggtggc gcgaggcggc ttcgatttta ctttgcgtca
     3001 gccgatgggg gtggtggcgg cgattgtgcc gtggaacttt ccctttccga ttgcgacatg
     3061 gaaggttgcg ccagcactgg cggcgggaaa ttgcgttgta ctcaagcctg cgcgccaatc
     3121 accgctcacc gccttgcgcc tcggagaatt ggctgcccag gctggtttgc cgccgggcgc
     3181 gctccaggta ttggccggat cgggagagca gatgggcgat gcgctggtga aacatccgtt
     3241 gattcgcaaa atttcattca ccggctcgac gtcggtcggt tcgcacatca tgcaattggc
     3301 ggcaagggac atcaaacgcg tctcgttgga acttggcggc aagtcgccca acattatttt
     3361 tgccgattgc gatttggaaa aagccgcggc cacttcgcca atgagtgtct ttgccaacac
     3421 cggccaggat tgctgcgcgc gcagccgcat ttttgtcgag cggtccgtgt atgaaaaatt
     3481 tgtggaatta t
//
LOCUS       DASZMM010000394         4678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_157515, whole genome shotgun sequence.
ACCESSION   DASZMM010000394 DASZMM010000000
VERSION     DASZMM010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157515"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2423)
                     /locus_tag="VG754_09580"
     CDS             complement(<1..2423)
                     /locus_tag="VG754_09580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19509.1"
                     /translation="MTRVKVAVVTRTKNRPLLLRRCVESVLNQTFQNWFHIIVNDGGD
                     PTTVEGVISGYAGRYNNRVRIIHNESSVGMQNASNQAIRATDSEYIGIHDDDDTWHGM
                     FLEECVGYLDAAESNSRDQGVATQSFWIMEEMDSWGNVIEISRQDFMPFESVNLFQAA
                     AKNPFPPIAFLYRRQVHETIGYFDQAFNELGDHDFNLRFLTHYDIGTIRKRLAGYHWR
                     HHATGSSYGNTVTDGVTSHRKMSVRMQNHYLRQDMANNRMGVGFLMNIASTMESQMEW
                     IHGLHKKSDHAAGIMSKVVRKMQYYERIVDTLITPWKGRTLPRRIYDHVTDLFTKRPE
                     SIKMPEKNNGHTSAAELESKLKNARILSLDVFDTALLRLVRKPTDVFLYIQQDVRRLL
                     NRPELGFVEARIAAEEESRQEMRRVRSIGETTLDDIYAVLRKRLGCDEKTASQIKQWE
                     LAAERHLCFANPHITRLVAQTRGGERRIVFCSDMYLPAREIAGLLEQNGFKDTSLFVS
                     SELWATKHEGPLFDQVTTATKCQPSEMLHIGDNPHSDVARPREKGIVAHHWTRAKSET
                     ALVDQHTSISGAWEGDLASSLYAGLVRRHRLEHPVEKAEGEAFWKMIGYEIVGPLHHA
                     YVSWVINRASKLGLKKLFFLARDGFNLLKTFDMFRKRDGLELEGEYLFASRRLWNFAR
                     IDRLDEENMVFLVVPNPMMRVRDFLARIDIDPEPHEALVRRMGFKGLNQRVTTEGGVF
                     KSETHHQNMRRLIHHFGGEILSHAQRERETLAKYFKDVGLLEDGAGIVDVGWHASSSR
                     SLQMLLAQSG"
     gene            complement(2430..3818)
                     /locus_tag="VG754_09585"
     CDS             complement(2430..3818)
                     /locus_tag="VG754_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF19510.1"
                     /translation="MSANNLTNAQPDRGACAIALRNVSKHYRLWHGTGPGRWARLLQL
                     FVTQPPVGEATRGVGEFFPALKNISFEVMPGESVGIIGRNGCGKSTLLQILAGIMKPT
                     SGSGTVNGELATLLELGAGFHPDFSGRENVALNSAVMNLSRVEAQARMEKIEAFADIG
                     RFMDEPIRTYSNGMLLRLGFACAVAVEPDVFLVDEALGVGDIFFQQKCYEYMREHMRG
                     KTRVVVSHDMHAITTFCSRVLVLEAGELIFDGPPLQAVELYMKALHDSQAGGSRNGSD
                     AGKNGPTERENAAMHTVQWRNIPAKSLGGIGDVQLTRAEVRVNGEPFTSVVKPGDVVS
                     VRALIQCRETVPSVLFGYLLRDKTGMPIFGENSAGVAGGIVTAKAGQSEFAFEFSWPE
                     VQPDDYFLTLGAGEGSDPIRHRIQCWAHNIHLFNGISPGKPVHCLFNNPLREFAFTPH
                     VDARAETILSQP"
     gene            complement(3835..>4678)
                     /locus_tag="VG754_09590"
     CDS             complement(3835..>4678)
                     /locus_tag="VG754_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF19511.1"
                     /translation="MEITSNPESFCRHLNPLRLGRELWNSRAVLVQFAGKEVLQRYRG
                     SQLGLVWIFLQPIFLLAVYTIAFGLILKTRWPESQRTGLGEVAMAVYCGLTAFGIFSE
                     CSARAPMLLLENASYVKRALFPIQMLPVCVVLSASFHCIVNLAILTALGWILSGFYAP
                     ALLAPIVLLPLVLLSCGATLLLAAVGPIFRDLRQFITPMLLAVSFLTPMFYSPKIVPA
                     RLDLLVNANPLAFTITTIRQLVMWHGSCDWLAWGIWTVVCAAFMILAHAIFMRLRHEV
                     TDLV"
BASE COUNT         1069 a         1400 c         1215 g          994 t
ORIGIN      
        1 ccggattgcg ccagcagcat ttgcaaggac cttgacgacg aagcatgcca gccgacatcc
       61 acaatgcccg cgccatcttc aagcagcccg acatccttga aatatttcgc cagagtttcc
      121 cgttcccgtt gcgcgtgcga caggatttcc cctccaaaat ggtggatgag ccggcgcatg
      181 ttttgatgat gcgtttccga tttgaagacg cctccctccg tggtgacccg ttggttaagc
      241 cctttgaaac ccatccgccg cacgagcgcc tcgtgcggtt caggatcgat gtcaatgcgg
      301 gcaagaaaat cgcgcacgcg catcatcggg ttcggcacga ccagaaaaac catgttctcc
      361 tcgtccagcc ggtcaatgcg cgcaaagttc caaagccgcc gcgacgcgaa cagatattcg
      421 ccctccagtt ccagtccatc ccgcttgcgg aacatgtcga aggtcttgag caggttgaat
      481 ccgtcgcgag cgagaaagaa gagctttttc aaacccagtt tggaggcgcg attgatgacc
      541 caggaaacat acgcgtgatg caatggcccc acgatttcat agccgatcat tttccagaaa
      601 gcctcgccct ccgccttttc aaccggatgt tccagccgat gacgacgcac cagcccggcg
      661 taaaggctcg aagcaagatc accctcccaa gcgccggaaa tcgaggtgtg ctggtccacc
      721 aacgcggttt cggatttcgc gcgcgtccaa tggtgcgcca cgattccttt ctcgcgcggg
      781 cgggccacgt ctgaatgcgg gttgtccccg atgtgcagca tctccgaagg ctggcacttc
      841 gtggccgtcg tcacctggtc aaacaacggg ccttcgtgct tggtcgccca caattcggag
      901 gagacgaaca atgaggtgtc cttgaagccg ttctgctcca gcaaacccgc gatttcgcgc
      961 gcgggaagat acatgtccga acaaaaaaca atccgccgtt cgccacccct cgtttgcgcg
     1021 acgaggcgtg tgatgtgggg attggcaaaa cagagatgcc gttccgccgc gagttcccat
     1081 tgcttgatct ggctcgcggt tttctcatca caaccgagac gcttccgcaa cactgcataa
     1141 atatcgtcga gggtggtttc gccgatgctg cggacgcggc gcatctcctg gcgcgattcc
     1201 tcctctgccg caatccgtgc ttccacaaat cccaattccg gccggttcaa caatcgccga
     1261 acatcctgct ggatatagag gaatacatcc gtcggtttcc tgaccaggcg aagcaaggcc
     1321 gtgtcgaaaa catcgagcga aaggatgcgc gcatttttga gtttcgactc caactcggca
     1381 gccgaagtgt gcccattatt tttctccggc atcttgatgc tctcgggccg cttcgtgaac
     1441 agatcggtta catgatcgta aatccggcgt ggaagggtcc ttcctttcca cggcgtgatg
     1501 agcgtgtcaa cgatgcgttc gtaatattgc atcttgcgga cgaccttgga catgatgccc
     1561 gccgcgtgat cggatttttt atgcaggcca tggatccatt ccatctggct ttccatggtg
     1621 ctggcgatgt tcatcaaaaa accaacgccc atgcggttgt tcgccatgtc ctgccgcaga
     1681 taatggttct gcatcctgac cgacatcttg cggtgcgacg tcacgccgtc ggtcacggta
     1741 ttgccatagc tgcttccagt ggcgtgatgc cgccaatgat agcccgccag tctttttcga
     1801 atggtgccga tgtcgtaatg ggtgagaaac cgcaagttga aatcgtgatc gcccagttca
     1861 ttgaaggcct ggtcgaaata accgatggtt tcatgcacct gccggcgata taaaaatgcg
     1921 atgggcggga acggattttt tgccgcggct tgaaacaggt tcaccgattc gaatggcatg
     1981 aaatcctggc gcgaaatttc gatgacgttg ccccaggaat ccatttcctc catgatccag
     2041 aagctctgcg ttgccacgcc ttgatcgcgt gaattgcttt ctgccgcatc gaggtacccc
     2101 acgcattcct caagaaacat cccatgccag gtatcgtcgt cgtcatggat gccaatgtat
     2161 tcgctgtccg ttgcgcggat ggcctggttg ctcgcgttct gcatcccaac ggaagattca
     2221 ttatggatga tccgcacgcg gttattgtat cggcctgcat aacccgaaat tactccctcg
     2281 accgtggtgg gatcaccgcc atcgttcacg atgatatgaa accaattttg aaaggtctgg
     2341 ttcagcaccg attcaacgca acgccgcaaa agcagcggac ggtttttggt cctggttaca
     2401 acggcaactt ttactcgagt catgatgaat taaggctgtg acaaaattgt ttctgcccgc
     2461 gcatcaacat gcggcgtgaa cgcaaattcc cgcaacggat tgttgaacaa acaatgcacc
     2521 ggttttcctg gactgatgcc attgaacaaa tggatgttat gcgcccagca ttgtatccta
     2581 tggcggatgg ggtcgcttcc ctcacccgct ccgagcgtca aaaaataatc atccggctgg
     2641 acttcgggcc atgaaaattc aaaggcaaat tcagactgcc cagcctttgc tgtcacgatg
     2701 ccgcctgcaa ctccggcgct gttctcgcca aaaatcggca ttcccgtttt atcacgcaag
     2761 agataaccga acaacactga aggcaccgtc tcgcggcatt gaatcaatgc ccgcacggac
     2821 acaacgtcgc ccggcttcac gacgctggtg aaaggttctc cattcacgcg cacttcagcc
     2881 ctggtgagtt gcacatcgcc gatgccgcca agggatttgg caggaatatt gcgccattga
     2941 acggtgtgca ttgcggcgtt ttcccgctcg gttgggccat tctttccggc atcgctgcca
     3001 ttgcgcgacc cgccggcctg gctgtcatgc aaagctttca tgtaaagttc aacagcttgc
     3061 aatggaggcc cgtcaaaaat caattccccg gcttccagca ccagcacacg cgaacaaaag
     3121 gtcgtgatgg cgtgcatgtc atgcgacacc acgacgcgcg tttttccgcg catgtgttcg
     3181 cgcatgtatt cgtaacactt ctgctggaaa aaaatgtcgc caacaccaag cgcttcatcc
     3241 accaggaaca catccggttc gacggcgacc gcgcaggcaa aaccgagccg caacaacatg
     3301 ccgttcgaat aggtgcggat cggctcgtcc atgaaccgcc cgatgtccgc gaacgcctcg
     3361 atcttttcca tccgggcttg tgcctcgacg cgtgacagat tcatcaccgc gctgttgagc
     3421 gccacatttt cgcgtcctga aaaatccggg tgaaaccccg cccccaattc cagcaaggtg
     3481 gccagttcgc cgttcaccgt gccgctgccc gaggttggct tcatgatacc ggcaagaatc
     3541 tgcaacaacg tgcttttacc gcagccattc cgtccgatga tgccgacgct ttcgccgggc
     3601 atcacttcaa acgagatgtt tttgagcgcg ggaaagaatt cgcccacacc ccgcgtggct
     3661 tcaccaaccg gaggctgcgt gacaaataat tgcaacaacc gcgcccaacg ccccggaccc
     3721 gttccatgcc agagccggta atgcttcgac acattgcgca acgcaatggc gcaagcgccg
     3781 cggtcaggtt gggcattggt caaattgttg gcgctcatat tcgcagcatc agcactaaac
     3841 gaggtcagtg acctcatgcc ttaatctcat gaaaatcgca tgggccaaaa tcatgaacgc
     3901 agcgcagacc accgtccaga tgccccacgc gagccaatca catgaaccgt gccacatcac
     3961 caactggcgg atggtcgtga tggtgaatgc cagcgggttc gcattcacca gcaagtcaag
     4021 gcgcgccggg acaatctttg gactgtagaa catcggcgtc aaaaaggaaa ccgcaagcaa
     4081 catcggtgtg ataaattgcc gcaagtcgcg gaaaattggc ccgacggccg ccagcaacag
     4141 cgtcgccccg catgaaagca gcaccagcgg caacaagaca atgggcgcaa gcaacgctgg
     4201 cgcataaaag ccgctcaata tccatcccaa cgccgtcagg attgccaggt tcacgatgca
     4261 atggaaactc gccgacagca cgacgcacac gggcagcatc tggatgggaa acagcgcgcg
     4321 cttgacgtag cttgcgtttt ccagcagcag catcggcgcg cgcgcggagc attcgctgaa
     4381 aattccgaac gccgtcaatc cgcaatacac tgccatcgcc acttcaccca agccggtccg
     4441 ttgcgattcc ggccagcgcg tcttcaggat gagcccgaat gcgatggtgt aaaccgcgag
     4501 caggaaaatt ggctgcaaaa aaatccacac caagccgagc tgcgagccgc gataacgctg
     4561 cagcacctcc ttgcccgcga attggacgag gacggcccgc gaattccaaa gctcccggcc
     4621 gagtcgcaac ggattaagat ggcggcaaaa gctctctgga ttcgacgtta tttccata
//
LOCUS       DASZMM010000395         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_978828, whole genome shotgun sequence.
ACCESSION   DASZMM010000395 DASZMM010000000
VERSION     DASZMM010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978828"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VG754_09595"
     CDS             complement(<1..684)
                     /locus_tag="VG754_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWF19512.1"
                     /translation="MKTASAVNTLADANGMSSAQQHSALKTVDCDVAFDNLTRQLYST
                     DASLYQIAPMAVAFPRNAKQASTVIRAAADAGISIIPRGAGTGLVGGAIGEGLIVDFS
                     RHNRQITALNLERRSVRVGAGVVLDQLNAFLKPHGFCFGPDVATSSRATLGGMIANNS
                     SGAHVPFYGVTADHVLTLEIALADGRIEQAGAQNESLGVQRRALAEMARHHAAAIREH
                     WTPELLKRRP"
     gene            912..1451
                     /locus_tag="VG754_09600"
     CDS             912..1451
                     /locus_tag="VG754_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWF19513.1"
                     /translation="MLYPSPVVWTRLAVLALFFITFSAAAQNGQSFTLAWNPSPDPTV
                     AGYVVYYGTSSGNYTTRMDVGTNTTATITNLQPGVTYYFAVTDYNSDGVESAPSGEIS
                     LLVPGIVKLRPNGFPGGIMLILFPVLPSHWYEVQASTDLKTWATIGQTSVATSNVWMG
                     CADFEGSNLQHRFYRVRTH"
BASE COUNT          350 a          450 c          438 g          360 t
ORIGIN      
        1 gggccggcgt ttcaacagtt caggcgtcca atgttcgcgg atcgcggccg catgatggcg
       61 cgccatttcg gcgagcgcgc ggcgttgaac acccaacgat tcgttttgcg cgccggcctg
      121 ttcgatgcgt ccgtccgcca atgcgatttc aagcgtaaga acatggtcgg cggtgacgcc
      181 gtagaacggc acatgcgcgc cggaagaatt gttggcgatc atgccgccga gcgtggcgcg
      241 cgaacttgtg gcgacatccg gcccgaaaca aaaaccgtgc ggcttgagga acgcgttgag
      301 ttggtccagc acgacgcccg cgccaacccg cacgctgcga cgttcgaggt tgagcgcggt
      361 gatttggcgg ttgtgccggg aaaaatcaac gatcaatcct tcgccgattg cgccgccaac
      421 caagccggtt cccgcgccgc ggggaatgat ggaaatgccg gcatccgccg ccgcgcgaat
      481 caccgtggac gcttgcttcg cgttgcgcgg aaacgcaacg gccatcggcg cgatttgata
      541 aagcgaagcg tcggtggagt aaagctggcg cgtcagattg tcgaacgcga cgtcgcaatc
      601 caccgtcttc agcgcggaat gttgttgggc cgaactcatg ccgtttgcgt cggcgagcgt
      661 attgacggca gatgcggttt tcaagttctg tcgcagccgc gccattttgt cgcgaacaaa
      721 atcgaaaaaa attatcgcct agaaatttgg cgaatgaaga attcgatggt attgttcatc
      781 ttctcaattc acaaaacctt cctgactttg taggaacaac actgataact acccaagctc
      841 gaatccgaca caatttatgg ttgcccagcc ccttggaacg tcactcggat tcagctttcc
      901 tttgcacaaa tatgttgtac ccaagtcctg tcgtttggac ccgcctcgca gtgctcgcct
      961 tgtttttcat cacattcagc gctgcagccc aaaatggaca aagtttcact ctcgcttgga
     1021 acccgagccc ggacccaact gtcgccggct acgtggttta ctacggcaca tcgagcggaa
     1081 attacaccac gcgcatggac gtgggcacga acacaacagc caccatcacg aacctccagc
     1141 ccggcgtcac ttattatttt gcggtgacgg attacaattc agacggcgtg gaaagcgcgc
     1201 cttccgggga gatttcgctt cttgttcccg gcatcgtgaa actgcggccg aatggatttc
     1261 ccggaggaat catgctcatc ctgtttcccg tcctgccatc gcattggtat gaagtccagg
     1321 cttcgaccga tttgaaaact tgggcgacga ttggccagac atccgtcgcc acatccaatg
     1381 tttggatggg ttgcgccgat tttgaaggaa gcaatctcca gcatcgcttt tatcgtgtgc
     1441 gcacgcattg actggaacgg aaattttcgc gcgaaggccg cgaagtgtgc agaaaaaaaa
     1501 ttgaaaaacg ctttttacga atgcgttttc accgcgtttt cctcgatgcg gctgaggaat
     1561 ttctcgatgc cttcgacaca ccggtcgtaa tcgctgat
//
LOCUS       DASZMM010000396         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1295298, whole genome shotgun sequence.
ACCESSION   DASZMM010000396 DASZMM010000000
VERSION     DASZMM010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295298"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..650)
                     /locus_tag="VG754_09605"
     CDS             complement(246..650)
                     /locus_tag="VG754_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWF19514.1"
                     /translation="MKTSLKIPDPAKAKEFFEKKITFTTGPVEVDHMLKNHENINVID
                     VRAEEDYKKGHIPSAINLPREKWDTFEGLSKDKANILYCYSMVCHLAATAAYKFADRG
                     YPVMELDGGFDEWKEHGLDVETQANRGVSLNA"
     gene            938..2941
                     /locus_tag="VG754_09610"
     CDS             938..2941
                     /locus_tag="VG754_09610"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF19515.1"
                     /translation="MENEFLKILLIDSQPGFAGQLRDMLGQAKGASFELMFFNRFEEG
                     LARLAEGGIDLALIELCLPDGAGLANVQRAQAQDIRVPIIVLGDADDETVAIGAVHAG
                     AQDYLVKSQLNPQLLGRAIRYAIERQRADAALLEAEEKYRGIFENIVEGIFQTSPDGR
                     YLSANAALARIYGYTSPQELIESVRDIGRKLYVEQGRRAEFVQLMQQHDTVTDFESRI
                     FRKDGTIIWIAENVRAIRNEKNELLYYEGTVEDITQRQMAEEKLRESEALYHSLVENL
                     PQNIFRKDLQGKFTFVNQRFCNTIGRTAAEIIGKTDFDLFPDELATKYQKDDRRILEK
                     NKMFETVEQHQPPGGEMIYVQVVKTPLYDSQGKMIGLQGIFWDISERKRAEEQIRLAT
                     AELARSREALRKKNEQMEEDLKMAREIQQTMLPQQYPSFPKSAAPGHSRFRFSHRYLP
                     TGTVGGDYFNVMALSDSQAGVFICDVMGHGVRSALVAAMVRALVEELKPLGSDPGKLL
                     TQLNRDLCSILKHTGSPTLTTAFYLTADATTGLLRYANAGHPRPFLIHRGTNTTELLT
                     NGQGKKNPALGLFEDAAYATAQSPISEGDLVMMFTDGLYEVEGKEQTLYTSEMLADAV
                     RRRARLPAGELFDEVLAEIKEFALGQDFVDDVCLVGMEFSEGK"
     gene            complement(2970..3353)
                     /locus_tag="VG754_09615"
     CDS             complement(2970..3353)
                     /locus_tag="VG754_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HWF19516.1"
                     /translation="MSPPLSVRIDKWLWAVRVYKTRSVAAAACRNGRVTIGGQPVKAS
                     REVKINDLILAQNGDITRTVKVLALLDHRVGAPAVKEFAEDLTPASEYEKPKERYLEP
                     LFYRPKGAGRPTKKDRRAINKMLPE"
BASE COUNT          770 a          946 c          964 g          810 t
ORIGIN      
        1 gatcacacgc tggccatcgt tggcaagctg tttcaaagca atggcaatcg ccatcagttt
       61 ttttgccgtg gcgccaattt tattccattc gtcaatggtc gcggcgcggt ttggttgtgc
      121 tgtggcggca agttccatcg gacttgcaat gcttgatttg ggaaacggtg gattggacac
      181 cgatttcctc aggagcgccg ccgatttggt gttgttcaca ccgttgctgt ttcgactttt
      241 ttagctcacg cgttcaagct gacgccgcgg ttcgcttgag tctcgacatc caatccgtgc
      301 tccttccact cgtcaaaacc accgtccaac tccatcacgg gatatccgcg atcggcgaat
      361 ttataggctg ccgtggccgc gaggtggcaa accatgctgt agcaatacaa aatgttcgcc
      421 ttgtccttgc tcagaccttc aaatgtgtcc catttttccc tgggcagatt aatggcactt
      481 ggaatgtgtc ctttcttgta gtcctcctcg gcccgcacat caatcacgtt gatgttctcg
      541 tggttcttca gcatgtggtc aacttcgacc gggccggtcg taaacgtgat ttttttctca
      601 aaaaactctt ttgccttggc gggatcggga attttcaagc tggttttcat aaaaaacacc
      661 tctttcaaaa gacggaatta aagtttctcg cgcagcgaat ggatttcgct tcgcccaatg
      721 attcaactta gacccgcttt gcagtttttc aacgtctcat tttacttgcc agaaggccgt
      781 ttgtcttgat tagctggtaa gtggcggcgt gctccgttcg ccatgttgaa gtgcaaaacg
      841 atttgaaagc gaccgcgctt gggaaggccc gatccaggcg cgttgaaaag aaccgggcgg
      901 ttttgaaagc ccttatctaa aagggctgat tccgcccatg gagaacgaat tccttaaaat
      961 cctcctgatt gacagccagc ccggttttgc cggccaactg agagacatgc tggggcaggc
     1021 gaagggagcg agctttgaac tcatgttctt caaccgcttt gaggagggac ttgcccggct
     1081 ggctgaagga ggaattgacc tcgcgctgat tgaactgtgt ctgccggatg gagcgggact
     1141 tgccaacgtt caacgcgcgc aggcccagga catccgcgtt ccaatcattg tgctcggcga
     1201 tgcggatgac gaaacggttg cgatcggggc tgttcatgca ggcgcgcagg attatctcgt
     1261 caagagccag ttaaatcccc aattgctggg ccgcgcaatc cgttacgcca tcgagcgcca
     1321 acgcgccgat gccgccctgc ttgaagcaga ggaaaagtat cggggcatct ttgaaaatat
     1381 cgttgaaggc atcttccaga cctcgccgga cggacgttat ctcagcgcca acgccgccct
     1441 tgcccgaatc tatggttata cctcgccgca ggaattgatc gaaagcgtgc gggacatcgg
     1501 ccgcaaactt tacgtcgagc agggccgccg agccgagttt gtccaattga tgcagcagca
     1561 tgataccgtg accgatttcg aatcgcgcat cttccgcaag gacggcacca tcatctggat
     1621 cgccgagaac gtccgcgcca tccggaacga gaaaaacgaa ttgctctatt acgagggtac
     1681 agtcgaggac atcacccagc ggcagatggc cgaggaaaaa ttgcgcgaat cggaggcgct
     1741 ttaccattcg ctggtcgaaa acctgccgca gaatattttc cgcaaggacc ttcaaggcaa
     1801 gttcacgttt gtgaaccagc gcttttgcaa caccattgga cgcaccgccg cggaaatcat
     1861 tggcaagact gatttcgacc tttttccaga cgagctggcc acgaaatacc aaaaggatga
     1921 ccggcggatc ctggaaaaaa acaaaatgtt cgagactgtc gagcagcatc aaccaccggg
     1981 cggtgaaatg atttatgtgc aggtcgtcaa aacgccgctc tacgattcgc agggaaagat
     2041 gatcggtttg cagggcattt tctgggacat ctcggaacgc aagcgggccg aggaacagat
     2101 ccggcttgcg acggctgaac tcgcgcgcag ccgcgaagcg ttgcgcaaga aaaacgagca
     2161 gatggaagag gacctcaaga tggcgcgcga gatccagcaa accatgcttc cgcagcagta
     2221 tccttctttt cccaaatccg ccgcacccgg ccacagccgc tttcgctttt cccatcgcta
     2281 tctgccgacg ggcactgtcg gcggggatta tttcaatgtg atggctttgt cggactcgca
     2341 ggccggcgtt ttcatctgcg acgtcatggg ccatggcgtg agatcggcat tggttgccgc
     2401 aatggtccgc gcgttggtcg aagaattaaa accgctcgga agcgatcccg gcaaattgct
     2461 gacccagctc aatcgcgacc tctgctccat cctcaagcac accggttcgc cgacgttgac
     2521 aacggcgttt tacctgacag cggatgcgac gaccggtctt ttgcgttacg ccaacgcggg
     2581 gcatccgcgg ccgttcctga tccatcgcgg aaccaacacc accgaactgc tcaccaatgg
     2641 ccagggcaaa aaaaatcccg cgctcggttt gtttgaggac gctgcttacg ccactgctca
     2701 atctcccatt tctgagggcg accttgtcat gatgttcacg gatggcttgt atgaggttga
     2761 gggaaaggaa cagacgctct acacgagcga gatgctcgcg gacgccgtgc gccggcgcgc
     2821 gcgattgccg gccggtgaat tatttgacga ggtgcttgcc gagataaagg agttcgcgct
     2881 cggccaggat tttgtggacg atgtttgcct tgtgggaatg gaatttagcg aggggaaata
     2941 ggccgcctga gcggcaattt cagaaaggtt cactccggca acattttatt tattgcgcgg
     3001 cggtcttttt tcgtcggacg gcccgctccc ttgggacgat aaaacagcgg ttccaaataa
     3061 cgctccttgg gcttttcata ttccgaggct ggagtcaaat cttcggcaaa ttccttcaca
     3121 gccggcgcgc ccacccgatg atccagcaac gcaagcacct tgaccgtgcg cgtgatgtct
     3181 ccattctgcg cgagaatcag gtcgttgatt ttgacttcac gcgaagcttt gaccggttgg
     3241 ccgccgatgg tcacccggcc attgcggcaa gcggcagcgg cgacactgcg ggtcttgtaa
     3301 acccgcacgg cccaaagcca cttgtcaatg cggacggaaa gaggagggga catcggagga
     3361 gtcaggtaat ggagtggtgg aatcggggct taaacgagga cgctcaaatt ttttagcctc
     3421 agctcttgcg ccattccaat tccacgattt gtccgtgggc tttgtgagtg cggtcgaggc
     3481 agcttaattc
//
LOCUS       DASZMM010000397         5927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1811085, whole genome shotgun sequence.
ACCESSION   DASZMM010000397 DASZMM010000000
VERSION     DASZMM010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811085"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..1400
                     /locus_tag="VG754_09620"
     CDS             489..1400
                     /locus_tag="VG754_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWF19517.1"
                     /translation="MRGKIRVGTASWSDVEFINTWYPKGLPPAERLAWYAEHFDLVEI
                     NSTFYRVPSRENVQNWCEQTPDEFLFNVKLHRVLSRHSTAPGFLPPDLRPKAKTKKEK
                     VIVTPELEEEIADRFMEAVKPFEDTGKMGAFLLQLSPSFSPRTNQIGELSHLASCFRG
                     KQLAIELRNRAWLDAKHRRETEHWFRKNHVTFVMVDGPDDPHFMVLPSSDAITNPNLA
                     YIRAHGRNAPKYIRGRTVAERFDYDYRIAELKEMARRAARIVVHTDELHIVFNNNKAD
                     YAPKAAERFLQILSAKRAKSRREAAYA"
     gene            1393..2037
                     /locus_tag="VG754_09625"
     CDS             1393..2037
                     /locus_tag="VG754_09625"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWF19518.1"
                     /translation="MRNGAATVKEASAKSRAVIESDPNRAQSNRVKQLEKLDGQIRVC
                     TRCPLCESRTLAVPGEGKSTARVMIIGEGPGGQEDKTGRPFVGNAGKFLEHVLEGTGY
                     SREDFFITNMVKCRPPSNRTPKTGEIDTCTSLYLFKQMALINPALIVLLGNVAVKKML
                     GLKTVEEARGRIIQHDGAKYFATYHPAVRFYREDLSKKIHEDFELLKKEMKKLE"
     gene            complement(2060..2272)
                     /locus_tag="VG754_09630"
     CDS             complement(2060..2272)
                     /locus_tag="VG754_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWF19519.1"
                     /translation="MNDLGKMLVVFGSVIVVIGVILWSGFGRGWFGRLPGDIHVQKGN
                     FTFYFPVVTCILLSIILTIVMRIFRK"
     gene            complement(2409..4250)
                     /locus_tag="VG754_09635"
     CDS             complement(2409..4250)
                     /locus_tag="VG754_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF19520.1"
                     /translation="MSNHPHSNESADPTFAAKQRSTWEIVKRVAVFLKPYKLMSLGTM
                     ACALLSLAFSLAYPHFIGSLIDDVIQKKDTNHLTWVMVALLGSFLLRDLFNGIRILIN
                     NTLEQNVIFDMRCNVYARLQRLPVHYFDQRASGDLMTRVIEDVNSVERILIDGIEQGS
                     VALLSVFIVPVVLFRMSPILGFVAMVPVPLLMGGALWYTLTARKRYRAQRQAASAMNA
                     LLMDNLQGVRQIKSFGQETHEDGRFAGRADDLRKGTLAIMRVYSIYGPAMTFSAALGT
                     VLISWVGGWQVIHGALTPGQLVRFLFYLALFYEPIRALHGLNQLLQAARASGERVFDI
                     LDFAVERMDNKRRQSMATPVRGEVVYEKVGFGYSSDKEVLRGISLHARPGEMIALVGP
                     TGAGKSTLVNMLPAFYEITSGCITIDGQDITGLTLESLRRQISIVSQEPFLFNGTIRE
                     NILYGNLSATEADLLAAAKAANCHEFIARLPTGYDSRVGERGVKLSVGEKQRVSIARA
                     LLKNAPILILDEATASVDTGTEKLIQEALERLMANRTSFVIAHRLSTIRKADQILVLR
                     DGEIVERGNHDELLALNGLYARLARIQNTVLIEEGFEKLSAVEQGAV"
     gene            5162..>5927
                     /locus_tag="VG754_09640"
     CDS             5162..>5927
                     /locus_tag="VG754_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWF19521.1"
                     /translation="MDGQTLELIHSAKAQQEFLLESHAQVRERVPGRILDEFQKTGDS
                     AEIENGVRKFVEWINSDKLEIRAFPSERLHAKLYVMTFVEGHIDKGRVVTGSSNFTEA
                     GLQDNLEFNVELKNRSDYEFALAKFNELWAKSVDVTKDYVTTIEVKSPFAQFTPYELY
                     LKFLYEYFRGELNLPDEIEDVYLPNGFKKLKYQEEAVLNARKVLDEYGGAFLSDVVGL
                     GKTYMSALLAQHLNEPCLVIAPPHLLDAHNPGSWPNV"
BASE COUNT         1450 a         1562 c         1585 g         1330 t
ORIGIN      
        1 attgttgcaa ttgcgaatcc attcgagaat gtaaaaatgc cgcgccactt tttgcaaggg
       61 gaaacggggt aaatactcaa actcgggcgc atccgcgcac tgccccggag tgcgggtcgg
      121 cgacccgcag caatgtggga agtatgagtg tatgaattag tctgggatgt ccctgttcgt
      181 cgcgcgttgc tgcggctcac cgagccgcgc tccgtttcgc ccggtgaatg cgacgggcaa
      241 tgacccggcc agtctctgcc ggcatgggca acaaatcggg gacagtgcgc ggatgtgccc
      301 cccaaactcc gaattatcca attagccaaa gaaatgtgag cctcggcaga ctccaggatc
      361 ggttgatgat tcgagcttgg atgctggagt ttgggatttc tttggttgct tgggtctttg
      421 ggtaattggg tatttattcc gcttgcggcc cgtcgtttca cgggggaaat taaaatacag
      481 gagcatttat gcgcggcaaa atacgggtcg gtaccgcgag ttggtcggac gtggagttta
      541 tcaacacatg gtatccgaag gggcttcctc ccgccgagcg attggcttgg tatgcggaac
      601 atttcgacct cgtcgaaatc aactcgacat tttatcgggt gccttcgcgg gaaaacgtgc
      661 aaaactggtg cgaacaaacg ccggatgaat ttcttttcaa tgtgaaactt catcgtgtcc
      721 tttcgcgcca cagcactgcg cccgggttcc ttcctcccga tctgcgaccg aaagcgaaga
      781 caaaaaagga gaaggtgatt gtcacgcccg agttggaaga agaaatcgcc gaccgtttca
      841 tggaagccgt gaaacctttc gaagacactg gcaaaatggg cgcgtttctt ctgcaacttt
      901 cgccgtcctt cagcccgagg acaaatcaga tcggcgagtt gagccatctg gcgagttgtt
      961 ttcgtggaaa acaattggcg atagaattgc gcaatcgcgc atggcttgac gccaaacacc
     1021 gccgcgaaac ggaacattgg ttccgcaaaa accatgtcac tttcgtgatg gttgatggac
     1081 ccgacgatcc gcatttcatg gtcctgccca gcagcgatgc catcacaaac ccaaatctcg
     1141 cttacatccg tgcccatggc cggaacgcgc cgaaatatat ccggggacgc accgttgccg
     1201 agcggttcga ttacgattat cgcatcgccg aactcaagga aatggcccgt cgcgccgccc
     1261 gcatcgttgt tcacactgat gagcttcata tcgttttcaa caacaacaaa gcggattatg
     1321 cgcccaaggc ggctgaaagg ttcttgcaaa tcctgtccgc caaacgcgcc aagagccgca
     1381 gggaggccgc ttatgcgtaa tggcgccgca accgtcaagg aagcctcggc caaaagccgc
     1441 gcggtcattg aatccgatcc gaatcgcgcg cagtccaatc gcgtgaagca gcttgaaaaa
     1501 ttggacggac aaatccgcgt ttgcacccgc tgtccgcttt gtgaatcgcg cacgttggcg
     1561 gttccgggcg aaggaaagtc caccgcgcgc gtgatgatca tcggcgaagg gccgggcggg
     1621 caggaagaca agaccggcag gccgtttgtc ggcaatgccg gcaaattttt ggagcacgtc
     1681 ctcgaaggca cgggttattc gcgcgaagat tttttcatca ccaacatggt caaatgccgt
     1741 ccgccctcca atcgcacgcc aaagaccggc gaaattgata cctgcacttc gctctacctt
     1801 ttcaaacaga tggcgctcat caacccggcg ctgattgtcc tgcttggcaa tgttgcggtg
     1861 aagaaaatgc tcggattgaa aaccgtcgag gaggcgcgcg gacgcatcat ccagcacgac
     1921 ggcgcgaaat acttcgccac ctatcatccc gccgtgcggt tctatcgcga agacctttcg
     1981 aaaaagattc atgaagattt cgagttgttg aaaaaggaga tgaaaaagtt ggaatgactg
     2041 tggattgtcg agaaggatgt catttcctga aaatccgcat gacgatggtc aagatgatgc
     2101 tcagtaaaat gcaggtcacc accgggaaat agaatgtgaa atttcctttc tggacatgga
     2161 tgtctcccgg caaacgaccg aaccagcctc gtccaaaacc agaccacaga atgacgccga
     2221 tcacgacgat gaccgacccg aataccacca acatttttcc caaatcgttc atgcttaaaa
     2281 ttcgctttca cttttcccat aggagccgac gtgaggaggc ccgcatttga tttgaaattc
     2341 aaatttcaaa tttgaaatca acacagattc cattgaagac tcgtaaatcg catatcgtaa
     2401 atcgtaaatc atacagcccc ctgctctacc gccgacaatt tctcgaaccc ttcttcaatc
     2461 aacaccgtgt tttggattcg cgccagtcgg gcataaagac cattcaacgc aagcagttcg
     2521 tcgtggttgc cgcgctcgac gatttctccg tcgcgcagaa caaggatttg gtcggccttg
     2581 cggatggtgc tcaagcgatg ggcgatgaca aaactggtgc gattggccat caaccgttcg
     2641 agcgcctcct ggatcaattt ttcggtgccg gtgtccacgc tggcagtggc ttcgtccaaa
     2701 atcaaaatgg gcgcattctt aagcaatgcg cgggcgatgc tcacgcgctg cttttcgccg
     2761 acgctcaatt tcacgccgcg ttcgccgacg cgcgaatcgt aaccggtcgg caatcgcgcg
     2821 atgaattcgt ggcagttcgc ggctttcgca gcggcaagca ggtcggcttc cgtcgcgctg
     2881 agatttccgt aaagaatgtt ttcgcggatg gtgccattga acaaaaacgg ttcctggctc
     2941 acgatgctga tttgccgccg caacgattcc agcgtaagtc ccgtgatgtc ctgcccgtcg
     3001 atcgtgatgc atcccgaagt gatttcgtaa aacgccggaa gcatgttgac gagggtggat
     3061 ttgcccgcgc ctgtcgggcc gaccaatgcg atcatttcgc cgggacgcgc gtgaagcgaa
     3121 atgccgcgca gcacttcctt gtcgctggaa taaccaaagc ccaccttctc ataaacgact
     3181 tcgccgcgaa ccggcgtcgc catcgattgc cggcgcttgt tgtccatccg ttcgaccgcg
     3241 aaatcaagaa tgtcgaacac gcgttcgccg gaagcgcggg ccgcttgcag caattgattc
     3301 aaaccgtgca acgcgcggat cggctcgtaa aaaagcgcaa gataaaacag gaaccgaacg
     3361 agttgtcccg gcgtgagcgc gccatgaatc acctgccagc cgccaaccca cgaaatcaac
     3421 accgtgccga gcgcggcgga aaacgtcatc gccgggccat agatcgaata cacgcgcatg
     3481 atggcgagcg tgcctttgcg caaatcatcc gcgcgaccgg cgaatcgccc gtcctcgtgc
     3541 gtttcttgcc cgaacgattt gatttgcctg acgccctgca aattgtccat caacagcgcg
     3601 ttcatggcgc tcgcggcctg gcgttgcgcg cggtatcgct tgcgggctgt caacgtgtac
     3661 cacaacgccc cgcccatcag cagcggaacg ggaaccatcg ccacgaaacc aaggatggga
     3721 ctcatccgaa acagcaccac cggcacaatg aacacgctca gcaacgcgac gctcccctgc
     3781 tcgattccat caatcaaaat ccgttccacc gaattgacat cctcgatgac gcgcgtcatc
     3841 aggtcgcccg aagcgcgttg gtcgaaataa tggaccggca gacgttggag ccgcgcataa
     3901 acattgcaac gcatgtcgaa gatgacgttt tgttccagcg tgttgttgat gaggatgcga
     3961 atgccgttga acaggtcgcg caacagaaac gagccgagca aagcaaccat gacccacgtg
     4021 agatggttcg tgtctttctt ttggatgacg tcgtcaatca acgagccgat gaaatgcgga
     4081 tacgcaagtg agaacgccag cgatagcaac gcgcaagcca tcgtgccgag cgacatcagt
     4141 ttgtatggct tcaaaaagac ggcgacgcgc ttgacgattt cccacgtcga gcgttgtttg
     4201 gccgcgaagg tcgggtcggc tgattcgttg ctatgtgggt gattggacat ctgtgacaga
     4261 atgaaacttc attttagtta cttaaacaca agtcggcgat aaggacagtg gatttttgca
     4321 atgccttgga tggcggttga gtttcataaa aatatttttt tattttatcg cggtttatcc
     4381 cggtgaagcg cggacaaagc ggaacgatgc ggaaatttcc taatgaccaa agcggaacga
     4441 tgcggaaatt ttggaggcgc tggaagcgaa atgggaaaat tcgagcctcc ggaaaatcga
     4501 gttgcgacag aaatttaacc acgacaaagc cgcacaaagg gctttgtgca ggtgcgaccc
     4561 gatgctggaa gccttgcgaa atggagtatt ggagtggcgg agttttggag aaaacaaccc
     4621 ggaacccgga acgggcatgc ggaaatggag tgttggagtc atggagcatc ggggggaatg
     4681 gaaggtggga caccgcgctg cggcgtgctt gccactggct ggatgcctgt tgctggattt
     4741 ttcgccactt tgaacattaa acgttaaagt ttgaacctgg cagcatttcc cgtgtggaaa
     4801 cacttcgcgc tcctcctcgc acggcttgca gtatgtcaca ccccggcagg atgtccagag
     4861 ttttcgctac tacaaagttc acggtgcgtc tggcttttgc attgcgctag gtttttttta
     4921 gcatacgatt catctcataa ttcatagcca agcaatcgaa cgaggcgaaa caatgtccaa
     4981 tgatctgacc ttcattacaa acgaatctgg gaaaagcctt tgtgacagat tcagcgtatt
     5041 gctgggaaag aatacgcggt ttttcgattg tctggtcggc tactttttta tcagcggttt
     5101 tcacaggctc catccctcgc tcgcaaacac ggagaaaatt agggttcttg ttgggatcaa
     5161 aatggacggg caaactttgg aattgataca ctctgccaag gcgcagcagg aattcctttt
     5221 ggaatcacac gcgcaggtcc gggagcgtgt ccctggcagg attctcgacg aattccagaa
     5281 gacaggcgac agcgctgaaa tcgaaaatgg cgtccgcaag tttgttgaat ggattaattc
     5341 cgacaaactg gaaattcgcg ctttcccctc agagcgcctt cacgcgaaac tgtatgtgat
     5401 gacttttgtc gaaggccaca tcgacaaggg gcgagttgtc acaggttcaa gcaattttac
     5461 cgaagccgga ttacaggaca accttgaatt caacgtcgag ctgaaaaacc gttccgatta
     5521 tgaattcgca ctcgccaagt tcaacgagct ttgggcgaaa tccgtggatg taaccaagga
     5581 ttacgtcaca accatcgagg tcaaatcgcc atttgcgcag ttcacgcctt acgaactcta
     5641 cctgaaattc ctttacgaat attttcgcgg cgaactcaat ctgccggatg aaatcgaaga
     5701 tgtttacctg ccgaacggtt tcaaaaaatt gaagtatcag gaagaagccg ttttaaacgc
     5761 ccggaaggtt ttggacgaat acggcggagc gtttctttcc gatgttgttg gtttgggaaa
     5821 aacgtatatg tccgctttgc tcgcgcagca tttgaacgag ccgtgccttg tcatcgcgcc
     5881 gcctcatttg ctcgacgcac acaatcccgg ttcctggccg aatgtct
//
LOCUS       DASZMM010000398         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5392738, whole genome shotgun sequence.
ACCESSION   DASZMM010000398 DASZMM010000000
VERSION     DASZMM010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5392738"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VG754_09645"
     CDS             <1..186
                     /locus_tag="VG754_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19522.1"
                     /translation="LKLIVTGQAGTYRIVASTNLSLGLSNWTTVTTITNTGTSVQFTD
                     PNTANFPRRYYKAILNP"
     gene            247..825
                     /gene="coaE"
                     /locus_tag="VG754_09650"
     CDS             247..825
                     /gene="coaE"
                     /locus_tag="VG754_09650"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWF19523.1"
                     /translation="MRLYGLTGGIGMGKSTSEKLLRERGVAVIDTDLIARKLVEPGQP
                     ALVEIQMQFGQDIISPDGWLRRDELGRRVFRDPAARQKLEAILHPRIREIWTAQVAQW
                     RAENLPRAVVVIPLLYETNAEANFDAVVCVACSARSQQERLQARGLDDDQIQKRIAAQ
                     WPVQKKLALSDFVVWTEGQLEVHAAQLERIIP"
     gene            882..3080
                     /gene="glgB"
                     /locus_tag="VG754_09655"
     CDS             882..3080
                     /gene="glgB"
                     /locus_tag="VG754_09655"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWF19524.1"
                     /translation="MLLSQDELQSLVQARHRAPHELLGMHPLGDGSGVVVRALIPNAA
                     KVEIEPTHEKQKPKIQLEQLDRSGLYEGVSKAAKKVYAYDLVITDYQGRVRRTRDPYS
                     FLPTLGETDLYLFGKGDERRIYDKLGAQLRVIDGVPGTSFAVWAPNAQRVSVVSDSNG
                     WDGRYHPMRLLGASGVWELFVPGLGEGTHYKYEIKNIRGDLVLKADPYGFFFEAAPKN
                     ASIVWDNKKFKWTDDAWRKQRGERNHLKSPMSIYEVHPGSWRKKSIAESLSYRELAAM
                     LIPYVKQLGFTHVELLPVAEHAFYPSWGYQVTGFYSPTARYGTPDDFQFFVNAMHEAG
                     IGVIIDWVPAHFPRDDWALARFDGTALYEHEDPRKGAHQDWGTLIFNYGRHEVSNFLT
                     ANALFWCDRYHVDGLRVDAVASMLYLDYSRQEGEWIPNQYGGRENLEAIEFLKKFNYL
                     THTECPGVVTVAEESTAWPLVTRPPYLGGLGFSLKWNMGWMHDTLDYFSRDSVYRKYH
                     QNDLTFAMLYHHNENFVLPLSHDEVVHGKGSLLGRMPGDDWQKFANLRALLGYQWAFP
                     GKPLLFMGGEFGQRAEWNANAGLDWHLLDQGPYHRGLQRFVEDLNKLYLAEAALWEGD
                     YDPSGFYWIDCADHRNSVFSFARQNPDRTSELVVILNLTPVLRTNYRIGLPRPGVWRE
                     LLNSDAAIYGGGNQGNFGRVTAEDYNVHNQSYSAEFTLPPLSIMLFAPGA"
BASE COUNT          727 a          880 c          945 g          738 t
ORIGIN      
        1 ttgaagctca tagtgacggg tcaggccggc acttaccgga ttgtggcgtc cacaaacctt
       61 tcgctgggcc tcagcaactg gaccacggtg acgacaatca cgaacacggg aacgtcagtc
      121 cagttcaccg accccaacac ggcaaacttc ccgcgccgat attacaaggc gatacttaat
      181 ccttaagttg ctgccgcgct tgcagccttg cctctccgca tttgggagag acgatgacca
      241 aatcgcatga gactgtatgg gttgaccggc ggcatcggga tgggcaaatc cacctcggag
      301 aagctgctgc gcgaacgcgg tgtcgcggtg attgacaccg acctcattgc ccgaaagctc
      361 gtcgagcctg gtcaacctgc attggtggaa atccagatgc aattcggcca ggacatcatt
      421 tcccccgacg gctggcttcg ccgtgatgaa cttgggcgtc gcgtttttcg agatccagcc
      481 gcgcggcaaa aattggaggc catcctgcac ccgcgcatcc gtgagatttg gactgcccaa
      541 gtcgcgcaat ggcgggccga aaatttgcca cgggctgttg tcgtcattcc gcttctttac
      601 gagaccaacg ccgaggcgaa tttcgatgcg gtcgtttgtg tcgcctgttc tgccaggagt
      661 caacaggagc gtttgcaggc ccgcggcctg gacgacgatc aaatccaaaa acgcatcgcc
      721 gcgcaatggc ctgttcaaaa aaaactggca ttgtctgatt tcgtggtctg gacggagggc
      781 cagttggaag ttcacgccgc gcaactggaa cgtatcatcc cctgaatatg gggtgaattt
      841 tgagtttaaa ttctgttcaa gcctgttaaa tttccggtta catgcttctt tcacaggacg
      901 aactgcaatc tttagtccag gcacgccatc gggctccgca cgaacttttg gggatgcatc
      961 cactcgggga cggttccggc gtcgtggtgc gcgcattgat tcccaacgcc gccaaggtcg
     1021 aaatcgaacc gacgcacgaa aagcaaaagc ccaaaatcca actcgaacaa ttggaccgct
     1081 ccggtttgta cgagggcgtc agcaaggctg cgaaaaaagt ttacgcgtac gaccttgtca
     1141 tcaccgatta ccaagggcgt gtccggcgga cgcgcgatcc atattccttt ttgccgacac
     1201 tcggcgaaac ggatttgtat ctgttcggca aaggtgacga acggcgcatt tacgacaagc
     1261 tcggcgcgca attgcgtgtc atcgatggcg tgccggggac cagtttcgcc gtctgggcgc
     1321 cgaacgcgca acgcgtcagc gtcgtcagcg attccaacgg atgggatgga cgttaccatc
     1381 cgatgcggct gttgggcgcg tcgggcgtgt gggaattgtt cgtgcctggg ttgggcgagg
     1441 ggacgcatta caaatacgag atcaaaaaca tccgaggcga cctcgttttg aaggccgacc
     1501 cgtacgggtt tttctttgaa gccgcgccga aaaacgcctc catcgtttgg gacaacaaaa
     1561 aattcaaatg gaccgacgat gcctggcgca agcaacgggg cgaacgcaat cacctgaaat
     1621 cgccgatgag catttacgag gtgcatcccg gttcgtggcg aaagaaatca atcgcggaat
     1681 ctctcagcta tcgcgaattg gcggcaatgc tcatcccgta tgtgaaacag cttgggttca
     1741 cgcatgtcga actgctcccg gtcgccgagc acgcatttta tccctcatgg ggttatcaag
     1801 tgaccggctt ttattcgccc accgcgcgtt acgggacgcc ggatgatttc caatttttcg
     1861 tcaatgccat gcacgaagcg ggtatcggtg tcatcattga ttgggtcccg gcgcattttc
     1921 cgcgcgatga ttgggcgctg gcgcgtttcg acggcacggc gctttatgaa cacgaagacc
     1981 cgcgcaaggg cgcgcatcaa gattggggca ccttgatctt taattatggc cggcatgagg
     2041 tcagcaattt tttgacggcc aacgcgctgt tctggtgcga tcgttatcac gttgacgggc
     2101 tgcgcgtgga tgcggttgcc tcgatgcttt atctggatta ttcccggcag gaaggggaat
     2161 ggatcccaaa ccaatatggc ggcagggaaa atttggaagc catcgaattc ctcaagaaat
     2221 tcaattacct cacgcacact gaatgcccag gcgtggtgac cgtcgcggaa gaatccaccg
     2281 catggccgct tgtgacgcgt ccgccttatc tcggcggctt gggattctcc ctcaaatgga
     2341 acatgggctg gatgcacgac acgctcgact atttcagccg cgattcggtt tatcggaaat
     2401 atcaccagaa tgacctgacc ttcgcgatgc tttatcatca caacgagaat ttcgtgttgc
     2461 cgctttcgca tgatgaagtg gtgcacggca agggctcgtt gctgggacga atgccggggg
     2521 acgattggca gaaatttgcc aacttgcgcg cgctgctggg ttatcaatgg gcttttccag
     2581 gcaaaccgct gctcttcatg ggcggagaat tcggccaacg cgccgaatgg aacgccaatg
     2641 ccggccttga ttggcacctg ttggaccagg ggccgtatca tcgcgggttg cagcggttcg
     2701 tggaagattt gaacaagctc tatctcgccg aggctgcatt gtgggaaggc gattatgatc
     2761 cgagcggttt ctactggatt gactgcgcgg accatcgcaa tagcgttttc tcttttgccc
     2821 ggcaaaatcc ggaccggacg agcgaattgg tggtcatcct gaatctcacg ccggtgttgc
     2881 gcaccaacta tcgcatcggg ctgccgcggc cgggagtgtg gcgcgaattg ttgaacagcg
     2941 acgccgcgat ttatggcggc ggaaaccagg gcaattttgg gcgggtgacg gcggaagatt
     3001 acaatgtcca caatcagtct tactcggcgg agttcacgtt gccgccgttg agcatcatgc
     3061 ttttcgcgcc gggtgcctga aaaaagaatc tggcggacca ctttcgcggg ccgccaggaa
     3121 tatacaactt ttttgaagaa cgctacgccc aaggcgcggc attttttcgc ggatgcaaga
     3181 agggcctttt caccttcacc atgatgatga agacggagag tatgaaaaaa tctcttgtaa
     3241 tggagattcg tactttctgc atggcagcca ttaagcaagg cgcataccaa
//
LOCUS       DASZMM010000399         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5393059, whole genome shotgun sequence.
ACCESSION   DASZMM010000399 DASZMM010000000
VERSION     DASZMM010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5393059"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1448)
                     /locus_tag="VG754_09660"
     CDS             complement(180..1448)
                     /locus_tag="VG754_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19525.1"
                     /translation="MNASDTEPKQSVDLQTGPSTLKRYLFAFVATLIAFLIRKALDPA
                     LQHSTTKHIFITFIVATTLVAWYSGFVPSLLTFIAGFILADYFFLDPRHSFDVPTVDF
                     LQLVLPPVLVSMTIILFGRSMHLAREKANAHAREAINNQKKLESEVVERKRAEEEVRR
                     LNTELEERVQLRTAELLASNRDLESFTYSVSHDLRAPLRHVDGYAQMLVEEHGSQLPP
                     EALKLSQKIRQGSQNMGRLVDDLLNLSRVGKQPLAIKQVPLNALLNEAIAEIKPEIGD
                     RRVEWQIGELPAVQGDPGLLRQVFVNLLSNAVKYSRMSEISVVEIGEMAVDGEKAIFV
                     RDNGVGFNMKYASKLFGVFQRLHRVEEFEGTGVGLATVARIINKHGGKIWAEAEVNKG
                     AKFYFILPAVQRHVTPANAQPVPAMAMERI"
     gene            complement(1486..>2388)
                     /locus_tag="VG754_09665"
     CDS             complement(1486..>2388)
                     /locus_tag="VG754_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWF19526.1"
                     /translation="APNPNATPAKHMIVFLNGQFVLEEQALVSVLDRGFLYGDGVFET
                     LRIFRSQPFRWAEHIDRFQHGTAFLKIDLPFAPIELFAHAQRLIELNAMPESLLRITL
                     TRGVGARGYSPKGANRPTLAMTLHTAPEPKPGPPPAWRVVTASCRLPANEALAEFKTC
                     NKLPQILARAEADASGADEALLLNTDGNIVEASSSNLFWIANDSVCTSPLQSGVLAGV
                     TRLVVSEICKKLSLTACEEVITPAGLRRAQGAFLSLSSWGIVEIASLDGHPLARSPLT
                     AKIRAAYEDLVVCEIGASVGQRNL"
BASE COUNT          531 a          623 c          706 g          528 t
ORIGIN      
        1 ttgaagcgtt tggcgaccgc ttttcttgtt ttgatacctt tggggcgtct cataagttta
       61 ctttcgttac aaaagagcgg gcaatatagg ctgctccgca ggatgaatca aggggtattt
      121 tgagtttttt ggcacgcgaa aatgctgcga gtagcgggcg aaaccagcaa aagcgccgct
      181 caaatgcgtt ccatggccat ggcgggaact ggttgggcgt tggcaggcgt gacgtgcctt
      241 tgaacagccg gcaagataaa atagaacttg gcgcctttgt tcacctcggc ttcggcccaa
      301 atcttgccgc catgcttgtt gatgatgcgc gcgacggttg cgagaccgac gcctgtgccc
      361 tcgaattcct cgacacgatg cagccgttga aagacgccga acaatttact ggcatatttc
      421 atgttaaaac cgacgccgtt gtcgcgcacg aaaatggcct tttcgccatc cacggccatc
      481 tcgccaatct cgacgacgga aatttcgctc atacggctgt acttgacggc attggacaac
      541 aggttgacga agacttgccg cagcaggccc gggtcgcctt gaacggctgg taattccccg
      601 atctgccact ccacgcgccg gtcgccgatc tccggtttga tttccgcgat ggcttcattg
      661 aggagagcat tcagcggcac ttgctttatg gccaacggtt gcttgccgac gcggctcaaa
      721 ttcagcaaat cgtccaccaa ccggcccatg ttctggctgc cctgccggat tttttgcgag
      781 agcttcaggg cttcgggcgg caattgcgat ccatgttcct cgaccagcat ttgcgcataa
      841 ccatccacat gccgcaacgg cgcgcgcaaa tcgtgcgaga cggaataagt gaacgattcg
      901 aggtcgcggt tgctggcaag cagttcggcg gtgcgcaatt gcacgcgctc ttccaactcg
      961 gtgttcaatc ggcggacttc ttcctcggcg cgtttgcgtt cgacaacttc tgattcgagt
     1021 tttttctgat tgttgatggc ctcgcgcgca tgggcattgg ccttttcccg cgccaggtgc
     1081 atggaacggc cgaagaggat gatggtcatg ctgacgagca ccgggggcag aaccagttgt
     1141 aaaaaatcaa cggtggggac atcgaaagaa tggcgcggat ccagaaaaaa gtaatctgcg
     1201 aggatgaatc ccgctatgaa ggtcagtaac gaaggaacaa atccgctgta ccaggcaacc
     1261 agcgtggtgg ccacgataaa ggtgatgaaa atatgcttgg tagtgctgtg ttgcaaggcc
     1321 gggtccaggg ccttgcggat caggaaggcg atcaacgtcg cgacaaaggc gaaaagatag
     1381 cgtttaagcg tcgaagggcc ggtctgcaaa tcaaccgatt gctttggctc agtgtcggac
     1441 gcgttcattg aatgttattt atcgtaatga aaccaggcgc taaaatcaaa ggtttctttg
     1501 acctacagaa gcgccgattt cgcaaacgac aaggtcttcg taggcggcgc ggattttcgc
     1561 cgtcaatggc gaacgggcca acggatgacc atccaacgag gcaatttcca cgatgcccca
     1621 ggaactcaac gagagaaatg cgccttgggc gcggcgcagg cccgcgggcg tgatgacttc
     1681 ctcgcatgcc gttaaactca attttttgca aatctccgac acgacaagcc gagtcacacc
     1741 cgcgagaacg ccgctttgca acggtgaagt gcaaacggaa tcattcgcga tccagaaaag
     1801 attgctgctt gaagcttcaa caatgtttcc gtcggtgttc agcagcagcg cttcgtctgc
     1861 gcccgaggcg tcggcttcgg cgcgcgccaa aatttgcggc agcttgttgc acgttttgaa
     1921 ttcggccaac gcctcgttgg cgggcaggcg gcaggatgcg gtcaccaccc gccatgcggg
     1981 aggcggcccg ggttttggtt ccggcgcggt atgcaaggtc atcgcgagcg tcggacggtt
     2041 cgcgcccttg ggcgagtaac cgcgcgcgcc gactccgcgc gtaagtgtga tgcgcaagag
     2101 tgattcaggc atcgcattga gttcgatgag ccgttgcgca tgggcgaata attcgatcgg
     2161 cgcgaaaggc aggtcgattt tcaaaaaggc ggtgccgtgt tgaaaacggt caatgtgttc
     2221 ggcccagcga aacggttggc tgcgaaagat gcgcaatgtt tcgaataccc cgtcgccata
     2281 aagaaagccg cggtcgagca cggaaaccag agcctgctct tcgaggacga attggccgtt
     2341 gagaaagaca atcatgtgct ttgctggagt ggcgtttgga tttggggc
//
LOCUS       DASZMM010000400         4105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1560726, whole genome shotgun sequence.
ACCESSION   DASZMM010000400 DASZMM010000000
VERSION     DASZMM010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560726"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VG754_09670"
     CDS             <1..427
                     /locus_tag="VG754_09670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19527.1"
                     /translation="IVGRGDMGDVLRADIERLGLKGKAWLTPYCLDMPRAMNAIDCLV
                     HSQVGTEAFGLVLVEAFACGRPVIASDLDGIPEAFGVVNFGHLVKPESVDELARAMLD
                     WAQRPALNAQKRGELHEQVGQEYSLRAFGLRTMKLYETL"
     gene            complement(424..1428)
                     /locus_tag="VG754_09675"
     CDS             complement(424..1428)
                     /locus_tag="VG754_09675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19528.1"
                     /translation="MMTAPGSIGVLVPTFNCAGLVANHMESLNSWIDLATEVVVVDSD
                     STDGTVEQLKKNLRHPNVRFLQHPRGLYQSWNFGIGKLTTEYAYISTVGDSISREGLE
                     HLREIATAHECDVVLSKPRFVDNEGHSQGQTCWPIDDLRGHFPGQQPLVLEGLELSLL
                     VLANCTAAILGSSASNLYRTRCLQQRSFPTEYGTVGDGAWGLINAHDMRMGFTGEIFS
                     TFRDHPKAYSRSDYFVDSLHNKLFNLACESFKCHVAQSSEMQKRARMANADLIMQTLA
                     RHLACQCRLGKCRDMKLPWILNPAAWVARLDRDRQARKLTRLKVAALKNIRAHKPGAS
                     "
     gene            complement(1425..2084)
                     /locus_tag="VG754_09680"
     CDS             complement(1425..2084)
                     /locus_tag="VG754_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19529.1"
                     /translation="MELLARELPLRVFVETGTFKGESLRAALPFFKECHSVELSPEYH
                     AAALKNFKGCPGVHLHLGESPAWLRKERTRFAATPTLFWLDAHWCVAEDAAGENSQSP
                     LLGELEAIGSLHPQGVVLIDDARLYLCPPPAPHRASDWPDLHSIVQSLLSLSSNHRLM
                     VLNDVLVFYPAGLHSTMGQFAHEHGVDWLQLTNMARKHAAKKERIRRIKNIFNPFSQK
                     K"
     gene            complement(2338..3180)
                     /locus_tag="VG754_09685"
     CDS             complement(2338..3180)
                     /locus_tag="VG754_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWF19530.1"
                     /translation="MLKARIRNFLGRRLGFYKSFVPDGFSAINQRLPEDVFIVAYPKS
                     GVTWFQNLVCGLAYGLDSGMSPPWLVNDLVPDIHITRFYRRYATPMYFKSHCLPRPEY
                     RRVVYLLRDGRDVMVSFKHYEETLKRKTLDFLQFVTCEESLFPCKWHRHVEEWSKNPF
                     KAEMLVIKYEDLLEQPVPQLERFCEFVNLSRKRDEISEIVEEAQFQHLHDKEDKFRCD
                     RLEKWPAGKFFFRRGVVGSHKDEMPPEVLQAFLSDASETLRRHGYPVGTAVPAGVAMA
                     AAGR"
     gene            complement(3451..3618)
                     /locus_tag="VG754_09690"
     CDS             complement(3451..3618)
                     /locus_tag="VG754_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19531.1"
                     /translation="MQHTLARPWNLMFTRHSLAFNPSFFAGAGRKRGPLSFLLNKTAL
                     IMTNSASQTMG"
     gene            3617..>4105
                     /locus_tag="VG754_09695"
     CDS             3617..>4105
                     /locus_tag="VG754_09695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF19532.1"
                     /translation="MDKSALITAYTPTYNEAGQIREVLESIRWADEIIIVDSFSTDDT
                     VEIARKYGARVISQEFSGFGNLRNTALDAAKNDWMFSIDSDERCTPELAREIRRESRS
                     PRYDAYRVPRKSHFMGRWMRHSGWYPDYRQPQFFNRRRMRYRNDVVHEGFTLSGRLGY
                     LRE"
BASE COUNT          979 a         1091 c         1118 g          917 t
ORIGIN      
        1 gatcgtcggc cgcggcgaca tgggcgatgt gttgcgcgcg gacatcgaac ggcttggctt
       61 gaagggcaag gcatggctga cgccgtattg cctcgacatg ccgcgcgcga tgaacgcgat
      121 tgattgcctc gtgcattcgc aagtcggcac cgaggcgttc ggactggtgc tggttgaggc
      181 gttcgcctgt ggaaggccgg tgattgcgtc cgacttggat ggcatcccgg aagcatttgg
      241 cgtggtgaat ttcggccatt tggtcaagcc ggaatcggtc gatgaactcg cgcgcgcgat
      301 gctggactgg gcgcaacgcc ccgccttgaa cgcgcaaaag cgcggcgaac tgcacgaaca
      361 ggtggggcaa gaatactcct tgcgcgcctt cggcttgaga acgatgaaac tatacgagac
      421 cctctaggac gcgcccggtt tatgcgcccg gatattcttc aaagctgcga ctttcaacct
      481 ggtgagtttt cgcgcctggc ggtcccgatc aagcctggca acccaggctg ctggattgag
      541 aatccacggc aacttcatat cgcggcactt tccaagccgg cattggcagg caagatgtcg
      601 ggcaagcgtt tgcatgatga gatccgcatt tgccattctt gcgcgttttt gcatttccga
      661 tgattgcgca acgtggcatt tgaaactttc gcacgccagg ttaaaaagct tgttgtggag
      721 tgagtccaca aagtaatccg accgggaata agccttggga tggtctcgaa acgtggagaa
      781 aatctcgccc gtgaaaccca tccgcatgtc atgagcattg atcaatcccc acgcgccgtc
      841 gcccacagtg ccgtactcgg tggggaaaga acgctgttgc aggcatcgag tgcggtaaag
      901 attgctggcg gaacttccca aaatggcggc agtgcaattc gccagcacca gaagcgaaag
      961 ctcaaggcct tccagcacca gcggctgctg cccgggaaaa tggccccgca gatcgtcaat
     1021 gggccagcat gtttgccctt gagagtgtcc ctcgttgtcc acgaaacggg gtttgctgag
     1081 aacaacatca cactcatgcg ctgtggcgat ctcccgcaaa tgttccagcc cttcgcgcga
     1141 gatggaatct ccgactgtcg aaatatatgc atattcggtc gttaatttgc cgatgccgaa
     1201 attccaggac tggtaaagcc cccggggatg ttgcaggaag cgtacattgg gatggcggag
     1261 gttttttttt agctgttcaa cagtaccgtc tgtcgagtcg ctgtccacca caactacttc
     1321 agttgccaga tcaatccatg aattgagcga ttccatgtgg ttggcgacca gaccagcgca
     1381 gttgaatgtc gggaccagga caccaatgga gccgggagcg gtcatcattt cttttgggaa
     1441 aacgggttga aaatattttt tatccgccgg atgcgttctt ttttggccgc gtgcttgcgg
     1501 gccatattgg tgagttgcag ccagtcaacg ccatgctcat gggcaaactg gcccatcgtg
     1561 gaatgcagcc cggcggggta aaacaccaac acgtcattca agaccatcaa acggtgattc
     1621 gaacttaacg acaacaacga ttgaacaatt gaatgaagat caggccagtc tgatgcgcga
     1681 tgcggcgcgg gcgggggaca gagatacagg cgggcatcat ctatgagaac aacgccttga
     1741 gggtgcaaag aaccgatggc ttccagttca ccaagaagag ggctttgtga gttttcaccg
     1801 gcggcatctt ccgccacgca ccaatgagca tcgagccaga aaagggtggg tgttgcagca
     1861 aacctcgtcc tttccttccg caaccatgcg ggtgattcgc ccaagtgtaa atgaactccg
     1921 ggacatccct taaaattttt cagggcggcg gcatgatatt ccggcgataa ttccacagag
     1981 tgacattctt tgaagaaggg gagggcagcg cgcagggact cgcccttgaa cgtgccggtt
     2041 tccacaaaga ctctgagtgg cagttcgcgt gcaagcaact ccatcagtct ggggtcaaag
     2101 gaaaagctga ttgcgcccat gaaatttagt tgcaatgcga ttgatcgcga atttcaaata
     2161 tttcaggttt gggtggtgct gcgcaaagac tgagagcgat gcctcctcca aatactgtcc
     2221 ctgattgaaa gagggcgtga cgttggaaat ttttggcaac aacgcgggca tagtgtcctt
     2281 caatggtgct gccgttatct ggttcttctt ctgaacagcg cggcaggctc gcccatttta
     2341 acgtcctgcc gcagccattg caacacccgc cggcaccgct gttcccacgg ggtaaccgtg
     2401 gcggcggaga gtttcggaag catcgctcaa aaaagcctgg agcacctcag gcggcatctc
     2461 atccttgtgg ctgccgacga cgccgcgccg gaaaaagaat ttacctgccg gccatttttc
     2521 cagacgatcg cagcgaaact tgtcctcctt gtcgtgcaaa tgctggaatt gcgcctcctc
     2581 cacaatctcg cttatctcat cgcgttttct cgaaaggttc acaaattcac agaagcgctc
     2641 caattgggga acaggctgtt caagcagatc ttcatatttg atgacgagca tctccgcttt
     2701 gaacgggttc ttgctccact cctcgacatg ccgatgccat ttgcagggaa aaagtgattc
     2761 ttcgcaggtc acgaattgga ggaaatccaa ggtttttctc ttcaatgttt cttcatagtg
     2821 cttgaaggag accatcacgt cgcgcccatc ccgcagcaaa taaaccacgc gacggtactc
     2881 cggacgcgga aggcaatgcg atttgaagta catgggcgtt gcataacggc ggtaaaaacg
     2941 ggtgatatga atgtccggaa ccaagtcgtt caccagccaa ggcggactca tccccgaatc
     3001 gaggccatag gcaaggccac aaaccaagtt ttgaaaccaa gtcactccgg acttgggata
     3061 ggctacgatg aaaacatcct caggcagacg ttgatttatc gctgaaaaac catccggaac
     3121 aaaacttttg taaaaaccaa gacgtctccc gaggaagttt cgaatacgcg cttttagcat
     3181 acaaaatcaa cacacaaatc tttctgatac gagcgggttc ccaagcttcg cttggccatc
     3241 aaactccccg taattattgc ataaaaaagc atttacgcaa gttttttgaa cggcttcccc
     3301 cctgagttac tcatcctctc tgaagcgcgt catagctcaa gacgccgagc gattctttca
     3361 ggtttcaaat atttgcattt ttcagaattt cttgttaaag tccagcggcc ttttggaaga
     3421 acagacgttt gagttcaaaa aggccgggtt ttaccccata gtttgggatg ccgaattggt
     3481 cataatcagc gcagttttat tcagcaggaa gctcaagggg ccgcgcttgc ggccggcgcc
     3541 tgcaaaaaaa gacgggttaa acgccaggct gtgccgggtg aacatcaggt tccaggggcg
     3601 ggccagggta tgttgcatgg acaagtccgc attaataacc gcatacacgc ccacgtataa
     3661 tgaagctggg caaatccgtg aggtgctgga aagcatccga tgggccgacg aaatcatcat
     3721 cgtggattcc ttcagcacgg atgacacggt ggaaatcgcc cggaaatacg gcgcgcgcgt
     3781 catcagccag gaattcagcg gcttcggaaa ccttcgcaac accgcgctgg acgccgccaa
     3841 aaacgattgg atgttcagca tcgattccga cgagcgttgc acacctgaat tggcgcggga
     3901 aatccgccgc gaatcgcgct cgccgcgcta cgatgcctat cgcgtccctc gcaagagcca
     3961 tttcatgggc cgctggatgc gccattccgg ctggtatccc gactaccgcc aaccgcaatt
     4021 tttcaaccgc cgccggatgc gctaccgcaa cgatgtcgtg catgaaggat tcactctcag
     4081 cggacggctc ggctatcttc gtgaa
//
LOCUS       DASZMM010000401         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_739896, whole genome shotgun sequence.
ACCESSION   DASZMM010000401 DASZMM010000000
VERSION     DASZMM010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739896"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VG754_09700"
     CDS             <1..485
                     /locus_tag="VG754_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HWF19533.1"
                     /translation="LLINLRRLEKDDLELEGELPAVQLDVEGVDELVKVKEPLHYQLE
                     AQKIEDGILVQGSLAVNLDCECARCLKPFKHRLEFDNWTCHLPLEGEDKVEVSNDCVD
                     LTPYVREDILLGFPQHPLCKPECGGLPKKASAKTKKNAGTGQTKETSSAWAELNKLKF
                     "
     gene            491..679
                     /gene="rpmF"
                     /locus_tag="VG754_09705"
     CDS             491..679
                     /gene="rpmF"
                     /locus_tag="VG754_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWF19534.1"
                     /translation="MGVPKRKPSRSRMRQRRAYNSVLTLPQLSTCPQCAAPYIPHRVC
                     PACGFYKGRQVITITAGS"
     gene            737..1783
                     /gene="plsX"
                     /locus_tag="VG754_09710"
     CDS             737..1783
                     /gene="plsX"
                     /locus_tag="VG754_09710"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWF19535.1"
                     /translation="MRIVVDVMGGDHGCVVVIEGVKRALEADQKISALHLVGKEDEIQ
                     SALKATGLRDGRIRIVHASEVMTMEDKPTVALRKKKDSSMARAIELVADGKADAVISL
                     GNTGGILALSTLRLRPLEGVDRAGIAAVIPAPDNDFILLDAGANIECKPVHLMQYAVM
                     GSVYSRAILGYEKPRVGLLSNGTEEGKGTELTKEAYKLCKQIDINFVGNIEGHGLFAN
                     QADVVICDGFVGNIVLKTCESLALGMVTMLKRELSRNPKRQLGAYLAQNALRAIKRRM
                     DPEAYGGAPLLGLNGTVMKAHGSAREKAIMNAIRITTETIQHQITQVISNEIQRANER
                     LDKQGSEPTTTGAL"
     gene            1780..>2370
                     /locus_tag="VG754_09715"
     CDS             1780..>2370
                     /locus_tag="VG754_09715"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HWF19536.1"
                     /translation="MSPSIKKFKNPRAKYDFKGRTCSIAGVGSYVPERIVTNADLEKM
                     VDTTDEWITSRTGIKARRVAATHEFTSDLATNAAIKAMKMAGVKGEEIDLIIVATITP
                     DMPFPSTACLVQQKIGAYRAAAFDIEAACSGFIYALEIGQQFIMSRTYNTVLVIGAEK
                     LSSIVNWTDRNTCVLFGDGAGAAILQNDPNSHGLLTA"
BASE COUNT          569 a          622 c          697 g          482 t
ORIGIN      
        1 ctttgctgat caatttgcgt cgtttggaga aggatgattt ggagttggag ggcgaactgc
       61 ctgcggttca attggatgtg gaaggagtgg atgaactggt caaggtgaag gagccgcttc
      121 attatcaact cgaagcgcag aaaattgagg acggcatttt ggttcaaggc agtctggcag
      181 tcaacttgga ttgcgaatgc gcccgttgtc tcaagccttt caagcaccgg ctggaattcg
      241 acaattggac ctgtcacctg ccgttggagg gtgaggacaa ggtggaagtc agtaatgatt
      301 gcgtggactt gacgccttat gtccgggaag atattctcct tgggtttccg caacatccgt
      361 tgtgtaaacc tgaatgtggc gggttgccga aaaaggcgtc cgccaaaacg aaaaagaacg
      421 cgggcacggg acagaccaaa gagacatcgt ctgcttgggc cgaactgaac aaattgaaat
      481 tttaaagatt atgggtgtac ccaagcgtaa accgtcaaga agccgcatgc gccagcgccg
      541 ggcttataac agcgtgctga cacttcccca actgagcacc tgcccgcaat gtgccgcgcc
      601 ttatatcccg caccgggttt gtcccgcctg cggtttttac aagggccgcc aggtaattac
      661 catcacggcc ggatcctgat tcattgattg agggctgtgg aagcggacac cagttgttcc
      721 gcccctctcc ccatttatgc gaatcgttgt ggatgtcatg ggtggcgacc acggttgtgt
      781 ggtggtcatc gaaggcgtca aacgcgcgct ggaagcagac caaaaaatca gcgcgctcca
      841 tctggttggc aaagaggacg aaatccagtc ggctctcaag gcgaccggat tgcgagacgg
      901 acgcatccgc atcgtccatg ccagcgaggt gatgacgatg gaagacaagc cgaccgttgc
      961 gttgcgcaaa aagaaagact cttccatggc gcgcgccatc gagttggtgg ccgacggcaa
     1021 ggcggacgca gtgatttcgc tcggcaacac cggcggcatc ctggcgctgt ccacgttgag
     1081 gttgcgtccg ttggaaggcg tggatcgcgc gggcattgcc gctgtcattc ctgcgccgga
     1141 caatgatttt atattgctcg atgcgggcgc gaacatcgag tgcaagccgg tccacttgat
     1201 gcaatacgcg gtcatgggta gtgtttattc gcgggccatc cttggctacg agaagccgcg
     1261 cgtcggattg ctcagcaacg gcacggagga aggcaaggga acggaattga cgaaggaagc
     1321 gtataaactt tgcaagcaga ttgacattaa tttcgttggc aacatcgaag ggcacggctt
     1381 gttcgcgaac caggcggacg tggtgatctg cgacgggttc gtcgggaaca tcgttttgaa
     1441 aacgtgcgag agccttgcat tggggatggt aaccatgtta aaacgcgaac tgagccgcaa
     1501 tccgaagcgg caacttggag cgtatctggc ccaaaatgcg ttgcgggcca tcaagcgccg
     1561 catggatccg gaggcgtacg gcggcgcgcc gttgttgggt ttgaacggga cggtgatgaa
     1621 ggcccacggt tcagcgcggg aaaaagcgat catgaatgcc ataagaatca caacggaaac
     1681 catccagcac cagatcacgc aggtcatttc caacgaaatc cagcgcgcga acgagcggct
     1741 ggacaaacaa ggttccgaac cgaccaccac aggcgcacta tgagcccaag cataaaaaaa
     1801 tttaaaaacc cgcgcgccaa atacgatttc aaaggccgca cttgctccat cgccggcgtc
     1861 ggttcgtacg tgccggaacg catcgtcacc aacgccgatt tggaaaaaat ggtggatacc
     1921 accgacgaat ggatcaccag ccgcacgggc atcaaggcgc gccgcgtcgc tgcgacgcat
     1981 gaatttactt cggacctcgc aaccaatgcc gcgatcaagg cgatgaagat ggcgggcgtc
     2041 aaaggtgaag aaattgattt gataatcgtg gcgaccatca cgcccgacat gccgtttcca
     2101 tcaaccgcct gtcttgtgca gcagaagatt ggggcttatc gcgccgccgc attcgacatc
     2161 gaggccgcct gctccggttt catttacgcg ctcgaaattg gccagcagtt catcatgtcc
     2221 cggacttaca acacagttct cgtcattggc gcggaaaaac tttcttccat cgtcaattgg
     2281 accgaccgca acacctgcgt gcttttcggc gacggcgcgg gcgcggcgat tttgcaaaac
     2341 gacccaaact cgcacggctt gctaaccgcg
//
LOCUS       DASZMM010000402         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_160155, whole genome shotgun sequence.
ACCESSION   DASZMM010000402 DASZMM010000000
VERSION     DASZMM010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160155"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1823)
                     /locus_tag="VG754_09720"
     CDS             complement(168..1823)
                     /locus_tag="VG754_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19537.1"
                     /translation="MNHYDSTCITATNSKISSPTSSPVRDERKTSKLFAIFRKYSWLV
                     FALVFAWKVALFVTSVQPVPANDAYFYDGAVVHYLLHGGYYNPCVAQAFPVSGTQVFS
                     AYPPGYQLPLLCWMKVFGVSAPSAMALHITLFGAYLIVLLAIFRRLQVPAWCVNLAGG
                     FLLTFTFHDRPDSFAHLLGVLAVYSCVRSRRALGCAQGESSALWAWLMALFIVLAFCA
                     SLQIGGIYLLVVWIATFAACRMGKEPIPLVPLAVMALVPALLALFVKLELPTAYAGFM
                     ENVHQTPFLTGLRKPHWQEVMKIIRTIPGILVVTMMLAFSWFKRKEGIQSTLNNRYGM
                     ILTATLLPALGIMVASLTIIAPNTVAISNYLQPMIVAAYLGFCVSRQVEWNWLRLQVV
                     CLVPAILLGSVRAVGMTTWGLACAADVNYSAAVQRVDQELAHLPAQSKVVMSSAFLYA
                     ASTHDDLNLIHSDWMTRAQGDSLVSDSQALIALKPEKMILTQYDYHRRMKAVLERVKN
                     DPSLKDIQITNLAHTPPPDSIRPLQQVVQHVSWAPVIVNLEWR"
     gene            1924..>2596
                     /locus_tag="VG754_09725"
     CDS             1924..>2596
                     /locus_tag="VG754_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HWF19538.1"
                     /translation="MPPVAANSQTSTVPLCVDLDGTLIRTDVLWESLILLVKRNPFYL
                     LALPFWLLRGRAYMKQQIAARTELNAADLPYHEPFLEYLKSEHRKGRPLILATAADLR
                     LAKNVADHVGLFSEVIASNGATNMRGRNKGNTLSGRFGEKGFDYAGNSSVDIPVWQQA
                     REAIVVNANDHLAQRAGKIAAVGQVFNEPGSLLRTLVKALRPHQWVKNIIIFVPVLTS
                     HNLGNP"
BASE COUNT          636 a          719 c          705 g          536 t
ORIGIN      
        1 tcctttgttc cacacggcgg cgatcatgcc aaccagacct ccagttgtcg agtgttttta
       61 atcggctttt gttttttagc gcaactcggg gccgccgaag gaaatcggaa gccaatcgag
      121 attccaatcc cacaatcaag cgagtctgcg gctcataaag gggacaatta gcgccactca
      181 agattgacaa tcaccggcgc ccaggaaaca tgctgcacca cttgttgcaa gggccttatg
      241 gaatcgggcg gcggcgtgtg ggcgaggttt gtgatttgaa tgtccttcaa actcgggtcg
      301 tttttgacgc gttcgagcac ggccttcatg cggcggtgat aatcgtattg cgtcaggatc
      361 attttctccg gcttcaaagc gatgagcgct tgcgaatccg aaaccagcga atcaccctgc
      421 gcgcgcgtca tccaatctga atggatcagg ttgagatcgt cgtgggtgga cgcggcatag
      481 aggaatgcgg atgacatgac caccttggat tgcgcgggaa gatgcgccag ttcctggtca
      541 acgcgttgca ccgctgcgga ataattcaca tctgccgcgc aagccaggcc ccacgttgtc
      601 attcccaccg cgcggaccga gcccaacaaa atcgccggca cgaggcaaac cacttgaagg
      661 cgcaaccaat tccattcgac ctggcgcgaa acgcaaaaac cgagataagc cgcaactatc
      721 atcggttgca gataattgga aattgcgact gtgttgggcg cgatgatggt caggcttgcg
      781 accatgatgc ccaacgcagg caacaaagtt gccgtcaaaa tcattccata acgattgttc
      841 aacgtggatt gaatgccttc ctttcgttta aaccatgaaa acgccaacat catagtcaca
      901 acgagaatgc cgggaatggt gcggatgatt ttcatcactt cctgccaatg cggcttgcgc
      961 aacccggtga gaaaaggcgt ttgatgaaca ttttccatga acccggcata ggcagtcggc
     1021 aactccaatt taacgaacag cgccagcaat gccggcacca gcgccatgac cgccagtgga
     1081 accagcggga ttggttcttt tcccatgcgg caggcggcga atgttgcaat ccagaccacc
     1141 agcagataaa tcccgccgat ctgcaaactt gcgcaaaacg ccagaacgat gaacagagcc
     1201 atcagccagg cccaaagggc ggacgattct ccttgggcgc aacccagagc gcgacgcgaa
     1261 cgcacgcatg aatacaccgc cagcacgcca agcagatggg caaaactgtc gggccggtcg
     1321 tgaaacgtga acgtgagaag aaatccgccc gcgagattca cgcaccaggc cggaacttgc
     1381 agccgccgga aaatcgcgag caacacaatc aggtaggctc caaagagggt gatgtgcaaa
     1441 gccattgccg acggcgcgga cacgccgaaa actttcatcc agcacagcag cggtaattga
     1501 taccccggcg gatatgcgct gaacacttgc gtcccggaaa ccgggaaagc ctgcgcgacg
     1561 catggattgt aatatccgcc gtgcaacaga taatggacaa ccgcgccgtc gtagaaatag
     1621 gcgtcattcg ccggcaccgg ctggacggag gtgacaaaga gcgcaacttt ccaggcaaag
     1681 accagcgcaa aaacgagcca tgaatatttg cgaaagatgg caaataattt ggacgttttc
     1741 ctttcatccc ggacgggtga tgaagtcggc gatgagattt tggaattggt tgcagtaata
     1801 caggttgaat cgtaatggtt catcccactt tggcaagccg cgattagttt tggtttctca
     1861 aactcccttg gctcttgtca accagcgctg cgctcggtta aggtgattaa tagtaaaaaa
     1921 ttcatgcctc ctgtggccgc caattcgcaa accagcaccg tcccgctttg cgtggacctc
     1981 gacggaacgc tcatccggac ggacgtgctc tgggaatcct tgatactgct ggtcaagcgc
     2041 aatccctttt atctgcttgc gctgccgttt tggctgttgc gcggtcgtgc ctacatgaaa
     2101 cagcagatcg ccgcgcgcac cgagctgaac gcggctgacc ttccctacca cgaacctttt
     2161 ttggagtatt tgaaatccga acaccgcaag gggcggccgc tcattctcgc cacggcggcg
     2221 gatttgcgcc tcgcaaaaaa tgttgccgat catgtcgggc ttttttccga agtcatcgcc
     2281 agcaacggcg cgacaaacat gcgcggacgc aacaaaggca acactttgtc cggacgtttt
     2341 ggcgagaagg gcttcgatta cgccggcaat tcctcggtgg atattcccgt ttggcaacag
     2401 gcccgcgaag ccattgttgt caatgccaac gaccatctcg cccagcgcgc cgggaaaatc
     2461 gcggcggtgg gacaggtgtt caacgagccg ggttcgcttt tgcggacgtt ggtgaaggcg
     2521 ttgcgaccgc atcaatgggt caagaacatc atcatttttg tgccggtgct gacttcgcac
     2581 aatcttggca atccgc
//
LOCUS       DASZMM010000403         8377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1298509, whole genome shotgun sequence.
ACCESSION   DASZMM010000403 DASZMM010000000
VERSION     DASZMM010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298509"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /gene="mutS"
                     /locus_tag="VG754_09730"
     CDS             <1..1914
                     /gene="mutS"
                     /locus_tag="VG754_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWF19539.1"
                     /translation="GAAGAVLHYLTQHLRRDVTHLTSLSCYQTSDFLNLDITTLRHLE
                     ILEPLHRDAPRAATLYGALNRTVTPMGARRLRDWLSQPLADAGAIARRQDVVQRWMER
                     GHSLEQFRAQLAQVRDLERTTGRLSAGSGNARDLAALRMALEQIPALKKALSDLAEAG
                     ARLALKEEDPSLLASVSTEDQELKEAASGQGLLEELEGELVELPDLIELIARAIAEEP
                     PLALKEGGLIRDGFDAALDELRGASRSGKDWIAKLQQDEIARTGIASLKVRFNSVFGY
                     YIEVTRSNLDKVPPQYIRKQTIANGERFITPELKEMEGKILGAEERSLKLEYELFLKV
                     RESVLGRLSEILRSATALAQLDVLTSFAETARLFGYCRPTVAGEGVLTIRDGRHPVLE
                     QNLSEERFVPNDTNLGAETQIALITGPNMAGKSTYIRQVALLVLLAHTGSFIPAAEAR
                     IDLVDRIFTRIGASDDLARGQSTFMVEMSETANILNNATSRSLIILDEIGRGTSTFDG
                     LSLAWSIVEHLHNQAGAKTLFATHYHELTELAGRLPRLKNFNVAVREWNDQIVFLRKI
                     VAGGTDKSYGIQVARLAGVPKPVLERAKEILRNLEESELTPEGTVRQQARHKAEREKL
                     KKLAPPPQMDLFG"
     gene            1925..2701
                     /locus_tag="VG754_09735"
     CDS             1925..2701
                     /locus_tag="VG754_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWF19540.1"
                     /translation="MSPTEILGLGASAMAAGAINAVAGGGTLLTFPTLLFFGTPPIVA
                     NATSTLALTAGTAGSVYGYRRLLGAVKSWLWRFVPVSIIGGMLGSALLTHTSEKTFSK
                     LVPFLILFATLIFLAQGFFRRLVALDENVAKNTRHHTMIGAVAFQFLVAVYGGYFGAG
                     IGILMLASLGFIRLGNIYEMNALKTVLSSLINLVASIWFIFAGLIDWPKAAVMTIGAL
                     AGYFLGAHYSQRIPARRVRQIITSIGLILSAWTFYKEFLR"
     gene            3164..4300
                     /locus_tag="VG754_09740"
     CDS             3164..4300
                     /locus_tag="VG754_09740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19541.1"
                     /translation="MSRRHPVLFVETIGPDPNLVMPSVRLMEKSPEHPNVTRLRLQFP
                     CWRWGDGAYVDRMRRELVQEALRGPLAGQFQNPVQWFYDPMTVASFAGKMDEIATVYD
                     CMDEHSKFNEAHPELTRRETELLAKADVVFTGGRKLYESKRRHNSNCHFYGCGVDYEH
                     FGRALDAETSVPHDVKALSRPVLGYFGVVDERMDYELVAKLADANPKWSLAIIGPVLK
                     VPHHLLPRRPNIHWLGSRDYSQLPAYCKGFDLCLMPFALNAATEYINPTKALEYMATG
                     RMIVSSAVPDVVSNFSSVVKVASSHEEFVTLCREAVERSEPALIERGLKMAAGNTWDS
                     IVAKMERHIDEALAAKRAHPAAHNHGKSRNLKNTRSQRVKAGAI"
     gene            4301..5617
                     /gene="glf"
                     /locus_tag="VG754_09745"
     CDS             4301..5617
                     /gene="glf"
                     /locus_tag="VG754_09745"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HWF19542.1"
                     /translation="MDTFDYLIVGAGYAGSILAERLARGCGKRVLLVDRRSHIAGNAY
                     DHYNNDGILVHRYGPHIFHTNSQEVFDYLSRFTEWRQYQHRVLACVDGQLVPIPINLD
                     TINQLYGLHLSSLQMDDFLASRAEKREPVRTSEDVVVNKVGRELYEKFFRGYTRKQWG
                     LDPSELDAQVTARVPTRTNRDSRYFTDSYQAMPLHGFTRMFEKMLDHPNIKILLNTDY
                     REVKNMVPYRELIYTGPVDEYFDFEFGKLPYRCLEFKHETHNQEFLQPVAVINHPNDY
                     AYTRVTEFKHLTGQVHPKTSIVYEFPRAEGDPYYPIPRQENATLYKKYQTLAENTQGV
                     HFVGRLATYRYYNMDQVAAQALTVFANLIGANRAKALAMEFEPSLATMTFPMSFNGKR
                     NGNRNGNGNGNGRHAARPSALGRRQRRSPVTNGNGNGNGNGHANGH"
     gene            5698..6945
                     /locus_tag="VG754_09750"
     CDS             5698..6945
                     /locus_tag="VG754_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HWF19543.1"
                     /translation="MTSTPKRKKSASSLAKDAGSLFQSFFLAGFECSTHRLRSGRRLD
                     MIDATQHDVFARQDYARINSQGIRATREGMRWHLAEKKPGRYDFTCARSIAEAAETTG
                     TQVIWDLCHFGWPDDIDILKPEFITRLADFAAAFAKFLAKESSLPPFFVPVNEISFFS
                     WAGGEEGKLNPFVTGRGFDLKCQLVRASIAAMDAVRSVDPKARFVHVDPIIHVVAHPD
                     RPEDTELAEAYRLSQFQAFDMLAGKLAPELGGGKKYLDIIGVNYYIHNQWIYDIKGFR
                     RSHEFEPLDRTDPAYRPLREILQEVYERYQRPLFLAETGAEDDARAPWLRYVTEEVEA
                     AITSGVPLDGVCLYPILNHPGWNDSRHCHNGLWDYTDAHGQRELYEPLAVELHRAQQR
                     MEKTVPSVSLATRSSHGAVGEKP"
     gene            7161..8129
                     /locus_tag="VG754_09755"
     CDS             7161..8129
                     /locus_tag="VG754_09755"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWF19544.1"
                     /translation="MNLLEVKNLKVHFPVKHGLFSRVKAYVKAVDDVSFSIQPGETLG
                     LVGESGCGKTTLGRAIVKLVEPTAGSILFEGEDIAHLSGAELRARRRKFQMIFQDPYG
                     SLNPRMTVGQIIGEAIDIHKLVESSSARQKRIVELLRAVGLDPTHAQRYPHEFSGGQR
                     QRIGIARALAVEPKLIVCDEPVSALDVSVQAQIINLLQDLQQKMGIAYLFIAHDLAVV
                     EHISRRVIVMYLGKVVELAEAKAIITAPKHPYTQALISAVPEVDPDTKRKRIVLPGDV
                     PSPINPPSGCPFHPRCPIAELPRCQVEIPPLREVTPGHWAACHFAK"
BASE COUNT         1881 a         2200 c         2439 g         1857 t
ORIGIN      
        1 ggagcggcgg gcgcggtgct gcattatctg actcaacatt tgcgccggga tgttacgcat
       61 ctgacgtcgc tctcatgtta tcaaacttcg gattttctga atctcgacat caccacgctg
      121 cggcatctgg aaattttgga accgctgcat cgggacgcgc cgcgcgcagc cacgctttac
      181 ggcgcgctca atcgcacggt cacgccgatg ggcgcgcggc ggttgcgtga ttggctttct
      241 caaccgctcg cggatgcggg cgcaatcgcg cgtcgtcagg atgtcgtgca aagatggatg
      301 gagcgcggcc acagtcttga acaattccgc gcgcaattgg cgcaagtgcg cgatctggaa
      361 cgaacaacag gccgtttgag cgccggcagc ggcaatgcgc gggatttggc cgccttgcga
      421 atggcgttgg aacaaattcc cgcgctgaag aaggcgcttt ctgatctcgc agaagccggc
      481 gcgaggctcg ctttaaagga agaagatccg agtctccttg cgtcggtttc tacagaagat
      541 caggagttga aggaagcggc ttccgggcaa ggcttgttgg aagagctgga aggtgaattg
      601 gtcgaattgc ctgatttgat tgagttgatt gcgcgggcga ttgccgagga gccgccgctc
      661 gccttgaaag aaggcggttt gattcgggac ggttttgacg cggcgctgga cgaattgcgc
      721 ggcgcatcgc ggagcggcaa ggattggatc gcgaagttgc agcaggacga aatcgcgcgc
      781 acgggcatcg cgtcgctcaa agtgcggttc aactcggtgt tcggctatta catcgaagtg
      841 acgcgcagca acctcgacaa ggtgccgccg caatacatcc gcaaacagac catcgcgaat
      901 ggagagcggt tcatcacgcc ggagttgaag gagatggaag gaaaaatcct tggcgcggag
      961 gaacgcagct tgaagctcga atacgaactg ttccttaaag tgcgcgagtc ggtgttggga
     1021 cggttgagtg aaatcttgcg gagcgcaacg gccttggcgc aattggatgt gctgacatcg
     1081 ttcgcagaga cggcgcggtt gtttggttat tgccgtccaa ctgtggctgg cgaaggggtt
     1141 ttaacaatcc gcgacggaag acatccggtt ctggaacaaa atttaagtga ggaacggttt
     1201 gtgccgaatg acacgaacct cggtgcggaa actcaaatcg ctttgatcac cgggccgaac
     1261 atggcgggca aaagcactta catccggcag gtggctttgt tggtgttgct cgcgcatacc
     1321 ggttcgttca ttcctgcagc ggaagcgcgg attgatttgg tggaccggat tttcacacgc
     1381 atcggggcga gcgacgacct ggcccgcgga caatccacgt tcatggtgga aatgagtgaa
     1441 accgcaaaca ttttgaacaa tgcgacctcg cgcagcttga tcatcctcga tgaaatcggt
     1501 cgcggcacga gcacgtttga tggattaagc ctcgcgtggt ccattgtcga acatctgcac
     1561 aaccaggccg gggccaagac gctgttcgcg acgcattatc acgagctgac ggaattggcg
     1621 ggacgtttgc cgcgtttgaa aaatttcaac gtggccgtgc gcgaatggaa cgatcaaatc
     1681 gtttttttgc gcaaaatcgt cgcgggcggc acggataaaa gttatggcat ccaggtggcg
     1741 cggcttgcgg gcgtgcccaa gccggtgctc gaacgcgcga aggaaatttt gcgcaacctg
     1801 gaggaatctg aattgacgcc cgaaggcacg gtgcggcagc aggcgcggca caaggcagag
     1861 cgggaaaaat tgaagaagct cgcgccgccg ccgcagatgg atttgttcgg ctgaaagtcg
     1921 aagaatgtca cccacggaaa ttttgggact cggcgcatcg gcgatggcag cgggcgccat
     1981 caatgccgtc gcgggcggtg gaacattgct gacatttccc acgctgcttt tcttcggcac
     2041 accgcccatc gttgcaaatg cgacgagcac gctggcgttg acggctggca cggcgggaag
     2101 tgtttacggt taccggcggc ttttgggtgc ggtgaaatcg tggctttgga gatttgtccc
     2161 ggtgagcatc attggcggaa tgttgggaag cgcccttctc acgcacacgt ccgagaaaac
     2221 tttttccaag ctggtgcctt ttctgatttt atttgcaacg ctgatttttc tggcacaagg
     2281 atttttccgc cgccttgtgg ctcttgacga gaacgtcgca aaaaataccc gacaccacac
     2341 aatgatcggc gcggttgcat ttcaattttt ggtcgcggtt tacggaggct atttcggcgc
     2401 gggaatagga attctgatgc tggcctcgct tgggtttatc agattgggaa acatttatga
     2461 gatgaacgcg ctcaagacgg tccttagctc gttgatcaat cttgtggcgt caatctggtt
     2521 catttttgcg ggactgattg actggccgaa ggcggcggtg atgaccatcg gcgcgctggc
     2581 gggatatttt ctgggggcgc attattcgca acgcattccg gcgcggcgtg tgcgccaaat
     2641 catcacctcc attgggctca tcctctccgc ttggacattt tacaaagaat ttttacgtta
     2701 acctgattac ggtcggcatc cacgcttttt gcgacaaatc tggttgtacg tgttcacaac
     2761 aaacctgtta gagccgaaaa tttcaaaatc cacttatttt ctagggaaat accccattga
     2821 gagagaggca tttgtgaagt gtattgagtc aggtttgagc cgcgcggttc ggcccaaaag
     2881 ttagggtgtc cggccgccat acaaacacgg ttcgaacatt gaaataatat tctttatgtc
     2941 ttacgcgcca tatcggtccg atgcaaactc atccgccttc aatccaaacc atgccgagcc
     3001 cgatatttca atttcggaaa attcggcgcg gcgcgcagca gcacaaaagc gttcgcacac
     3061 aaaattgaaa aaaaagaacg cgccgtctgg ctatcccata atcgttcatt gtcatttgtg
     3121 ttgggattgg gtatggcagc ggccgcaaca atttgtctcg cggttgagcc ggcgtcatcc
     3181 ggtgctgttc gtcgaaacca tcgggccgga cccaaatctg gtgatgcctt cggtccgttt
     3241 gatggaaaaa agccccgaac atccgaacgt gacccggttg cgtttgcaat ttccgtgctg
     3301 gcgttggggc gatggcgcgt atgtggaccg gatgcgtcgc gagttggtgc aggaagcttt
     3361 gcgcggtcct ttggccgggc aattccaaaa cccggtgcaa tggttttatg atccgatgac
     3421 agtcgcttcg ttcgccggga aaatggatga aatcgccacg gtgtacgact gcatggatga
     3481 gcattccaaa ttcaatgaag cccatcccga attgacgcgg cgcgaaaccg agttgctggc
     3541 caaggcagac gtggtcttca ccggcggccg gaaattgtat gaatcgaagc ggagacacaa
     3601 ttcgaattgt catttctacg gctgcggggt ggattacgaa cattttggcc gggcgctcga
     3661 tgccgaaaca tccgtgccgc atgatgtgaa agcgttgagc aggccggttc tgggatattt
     3721 tggagtggtg gatgaacgga tggattacga attggtggcg aagctggccg atgccaatcc
     3781 gaaatggtcc ctggcgatca tcgggccggt gttgaaagtg ccccatcatc tcctgccccg
     3841 ccgtcccaac atccactggc ttggcagccg cgattactcg caattgccgg cgtattgcaa
     3901 ggggttcgat ctttgcctga tgcccttcgc gttgaatgcg gcgacggaat acatcaatcc
     3961 aaccaaggcg ctcgaataca tggcaacggg ccggatgatt gtcagttcgg ccgtgccgga
     4021 cgtggtgagc aatttcagct cggtcgtgaa ggtcgcctct tcccatgagg aattcgtcac
     4081 actttgccgc gaagcggtgg aacggtcgga accggcgctc attgagcgcg gattaaaaat
     4141 ggccgcggga aatacgtggg attccatagt ggcaaaaatg gagcggcata ttgatgaagc
     4201 gcttgccgcc aaacgcgccc accccgccgc gcacaatcac ggcaaatctc ggaacttaaa
     4261 aaacactcgt tcgcaacggg taaaggcggg ggcaatctga atggacacat ttgattatct
     4321 gatcgtcggg gccggttacg cgggaagcat cctcgcggaa cggcttgcgc gggggtgcgg
     4381 caaaagggtg ttgctcgtgg accgccgttc gcacatcgcc ggcaacgcat acgaccatta
     4441 taataacgac ggcattttgg tgcatagata cgggccgcac attttccata cgaacagcca
     4501 ggaagtgttc gattatcttt cgcgcttcac cgagtggcgg caatatcagc accgcgtcct
     4561 tgcctgcgtt gacggccaac tcgttccgat cccgatcaat ctcgatacca tcaaccagct
     4621 ttacggtttg catttaagct cgctgcaaat ggatgatttt cttgcgagcc gcgctgaaaa
     4681 acgcgagccg gtgcgcacct ccgaagatgt cgtggtcaac aaggttgggc gcgaattgta
     4741 tgaaaaattt ttccgtggtt acacccgcaa gcaatgggga ctcgatcctt cggagcttga
     4801 cgcccaagtg accgcccgcg tgccaacgcg gacgaaccgc gattcgcgct acttcacgga
     4861 cagttaccag gcgatgccgt tgcacggatt cacgcgcatg tttgagaaga tgctcgatca
     4921 tcccaatatc aaaatcctcc tcaacaccga ttaccgcgaa gtgaagaaca tggttcctta
     4981 ccgcgagttg atttacaccg ggccggtgga tgaatatttc gatttcgagt tcggcaaact
     5041 tccctatcgc tgccttgagt tcaaacatga gacgcacaac caggaatttt tgcagccggt
     5101 cgcggtcatc aatcatccca acgactatgc ctacacgcgg gtgacagaat tcaaacacct
     5161 gaccggccag gtgcatccaa agacgagcat cgtgtatgaa ttcccgcgcg cggagggaga
     5221 cccgtattat ccgattccac gccaggagaa cgcgacgttg tataaaaaat atcaaacgct
     5281 ggcggagaac acccaagggg tgcattttgt gggcaggttg gccacgtatc gttactacaa
     5341 catggaccag gtggcggcgc aggctttgac agtttttgcc aacctgatcg gcgccaaccg
     5401 cgcgaaggcc ctcgcaatgg aattcgagcc gagccttgcc acaatgacct tcccgatgag
     5461 cttcaatgga aaaaggaacg gcaacagaaa tggcaatggc aacggcaacg gtcgccatgc
     5521 ggcgcgtccg tctgccttgg gacggcgcca gcgcaggtcg cccgtcacaa acggcaatgg
     5581 caacggtaat ggcaacggcc atgccaatgg tcattagcca aaaacccaca actctccagg
     5641 gacatgactg caactcccag tacacgaact cgagtgcgcc ccgccaaggc agcattgatg
     5701 acttcaacgc ccaagcggaa gaagtccgca tcgagtttgg cgaaagatgc ggggtcgttg
     5761 ttccaaagtt tttttcttgc cggctttgaa tgttcaacgc accggctgcg gagtgggcgg
     5821 cggttggaca tgattgatgc cacccaacat gacgtgtttg caaggcagga ttacgcgcgc
     5881 atcaacagcc agggaatccg cgccacccgc gaaggcatgc gctggcatct cgcggaaaaa
     5941 aaaccggggc gatacgattt cacctgcgcg cgttccattg cggaggctgc ggagacgacc
     6001 ggcactcaag tcatctggga cctttgtcat tttggctggc cggacgacat cgatattctc
     6061 aagccggaat tcatcacgcg cttggcggat ttcgccgccg cattcgcaaa attcctggca
     6121 aaagaaagtt ccctgccgcc gttctttgtg cccgtgaatg agatttcgtt cttttcctgg
     6181 gcgggcggcg aggaaggaaa gttgaatccg tttgtgaccg ggcgcgggtt cgatttgaaa
     6241 tgccagttgg tgcgcgcgtc catcgccgcg atggacgccg tccggtcggt ggatccgaag
     6301 gcccgtttcg tgcatgtgga cccgatcatc catgtggtcg cgcatcctga ccggcccgaa
     6361 gacacggaat tggcggaagc gtaccggctc tcgcaattcc aagcctttga catgctcgcc
     6421 ggaaaactcg cgccggaact gggcggagga aaaaaatatt tggacatcat cggcgtcaat
     6481 tattacatcc acaaccaatg gatttacgac atcaaggggt tccggcgttc gcacgagttc
     6541 gagccgctcg accgcaccga tccggcgtat cgtcccctgc gcgaaatttt acaggaggtt
     6601 tacgaacgtt atcagcgccc actgttcctg gcggaaactg gcgctgaaga cgatgcgcgc
     6661 gcgccctggc tgcgttatgt cacggaagaa gtggaagcgg cgatcaccag tggagttcct
     6721 ttggacggcg tctgtcttta tcccatcttg aatcatcccg gctggaatga cagccggcat
     6781 tgccacaatg gactttggga ttataccgat gcccatggtc agcgtgaact ttacgaaccg
     6841 ctggctgtgg agcttcatcg cgcgcaacaa cgcatggaga aaacggtgcc cagtgtttcg
     6901 ctggcgaccc gctcatcgca cggcgcggtt ggtgaaaagc cctgaccatt cgattcagcc
     6961 acggatgaaa cacggattga acacggagaa atgcaggcag aagcgcttct atccgtgttt
     7021 catcagtgtt caatccgtgg ctcaaccgca tcgttttcta caaagcaaat tttctcctgt
     7081 tattggcatt ggaagcatga attgacgcct ggcgtattct gcggtctatt gattccactt
     7141 ttgagcgaaa tcaaagcctc atgaacctgc tggaagtcaa aaatttaaaa gtccattttc
     7201 ctgtcaagca cggtttgttc agccgtgtga aggcatacgt caaggcggtg gacgatgtca
     7261 gtttttccat ccagccgggc gagacgcttg gattggtggg cgaaagcggt tgcggcaaga
     7321 caacgctcgg acgcgccatc gtgaaacttg tcgaaccgac ggctggcagc attctttttg
     7381 aaggtgagga catcgcgcat ttgagtggcg cggaattgcg ggcgcgtcgg cgcaagttcc
     7441 agatgatctt ccaggacccg tacggctcgc tcaatccccg catgacagtg ggtcaaatca
     7501 tcggcgaggc gatagacatc cacaagctgg ttgaaagcag ttcggcacgg caaaaacgga
     7561 ttgttgaatt gctgcgcgct gtcgggctgg atccaaccca cgcgcagcgt tatccgcacg
     7621 aattcagcgg cggccagcgc caacgcatcg gcatcgcccg cgcgcttgcg gtggaaccga
     7681 agttgatcgt gtgtgacgag ccggtcagcg cgttggacgt ttcggtgcag gcgcaaatca
     7741 tcaatctttt gcaggatttg cagcagaaga tgggcatcgc ctatctattc atcgcgcacg
     7801 accttgcggt ggtggagcac atcagccggc gtgtgatagt gatgtatctg ggaaaagtcg
     7861 ttgaactcgc ggaagcgaaa gccatcatca ccgcgcctaa acacccttat actcaggcgc
     7921 tgatttccgc agtcccggaa gttgatcccg ataccaagcg caagcgcatt gtgctgccgg
     7981 gggacgttcc ctcgccgatc aatccgccgt cgggttgtcc atttcatccg cgttgtccga
     8041 ttgccgaatt gccgcgctgc caggtggaaa ttccgcccct tcgtgaagtc acacccgggc
     8101 attgggcggc ctgccatttt gcgaagtgaa accattcaat tcagccacgg atgaaacacg
     8161 gattgaacac ggagaaatgc aggcagaaac catttttcaa gcaaggtggt caccttggtg
     8221 agtgcttttt ttgaaccgct caaaaatgac tgttctccgc tcctgtccgt gtttcatcag
     8281 tgtttcatcc gtggctgaat tgaatggttt cacttcgcaa aatgacacgc cgcccaatgc
     8341 ccgggtgtga cttcacgaag gggcggaatt tccacct
//
LOCUS       DASZMM010000404         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1812312, whole genome shotgun sequence.
ACCESSION   DASZMM010000404 DASZMM010000000
VERSION     DASZMM010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812312"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VG754_09760"
     CDS             <1..210
                     /locus_tag="VG754_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19545.1"
                     /translation="FTSVIMQDMPFFLDYRTYFITYDMDIWREAVAAKIAWWKIGESF
                     SLLVAYNLSFLIIGLTVFQMRDVKS"
     gene            364..>1656
                     /locus_tag="VG754_09765"
     CDS             364..>1656
                     /locus_tag="VG754_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HWF19546.1"
                     /translation="MGNAAPARPDLLAPVDTFPRRHIGPSSAETAQMLETLGFADLDA
                     LINTAVPAQIRLSRPLALPAGRGEQQVLQDLKVIASQNQVFRSFIGMGYSNCITPPVI
                     QRNVLENPGWYTQYTPYQAEIAQGRLESLLNFQTMISDLTGMDIANASLLDESTAAAE
                     AMHLCHDLHPERNVFFISSECHPQTIDVVKTRAKPLGIEVQVGDHETFEFNEKVFGAL
                     VQYPATYGDIYQYDGFVAKAHAVGAMVAVAADLLSLTLLRPPGEFGADVAVGSAQRFG
                     VPMGFGGPHAAYFATRDAFKRHMAGRIVGVSKDSRGRPALRLSLQTREQHIRREKATS
                     NICTAQALLANMAALYAVYHGPAGLKQIAKRVHLLTATLSKGLEQLGYQIKTNAFFDT
                     LRVDLGKKSSAEIIKLAESQRINLRTIDANTIGISLDEA"
BASE COUNT          359 a          495 c          430 g          372 t
ORIGIN      
        1 ttcaccagcg tcatcatgca agacatgcct ttctttctcg attaccgcac ctatttcatc
       61 acctatgaca tggacatctg gcgcgaagcg gttgcggcaa agattgcatg gtggaaaatc
      121 ggagaatcat tcagtctgct ggtggcatat aatctgagct tcctcatcat cggtctgaca
      181 gtcttccaga tgcgggatgt taaatcgtga ggctgattac aatgttaaat cgttccatgg
      241 ttacaagcag cagttgctcg cggtcgggca ggaccaaatc tcgcttgctt tgccgccatc
      301 ccaccttatt ttcattccat gtttgtctct gaagcaacag tcgccgccaa aaacaatgac
      361 ctgatgggca acgccgcacc cgcgcgtccc gatttgctgg cgccggttga tactttcccg
      421 cgccgccata tcggtcccag ctccgccgaa actgcgcaaa tgctcgaaac gctgggtttc
      481 gctgacctcg acgcgcttat caacaccgct gtgcctgcgc agatccgttt gtcgaggccg
      541 cttgccttgc ccgccggtcg cggcgaacaa caagtgctcc aggatttgaa ggtgattgcc
      601 agccaaaacc aggttttccg ttcgttcatc ggcatgggtt attcgaactg cataaccccg
      661 ccggtgatcc agcgcaatgt tttggaaaat cccggctggt acacgcaata cacgccttac
      721 caggcggaga tcgcccaggg ccgcctcgaa tcgctcctga atttccagac gatgatcagc
      781 gacctcaccg gcatggacat cgccaacgcc tcgttgctcg acgaaagcac cgccgccgcc
      841 gaggcgatgc atctttgcca cgatttgcat ccggaacgga acgtcttttt catctcgtcc
      901 gaatgccatc cgcaaacgat tgatgtcgtc aagacgcgcg ccaagccgct cggcatcgaa
      961 gtgcaggttg gcgatcacga aactttcgag ttcaacgaaa aagttttcgg cgcgttggtc
     1021 caatatccgg ccacctacgg cgacatctat caatatgacg gttttgtcgc gaaagcccac
     1081 gctgtcggcg cgatggtcgc tgttgccgcc gatttgctaa gcctcacgct gttgcgtccg
     1141 ccgggcgaat tcggcgcgga cgtggctgtc ggcagcgcgc aacggtttgg cgtgccgatg
     1201 ggttttggcg gaccacacgc ggcctatttc gcgactcgcg acgccttcaa acgccacatg
     1261 gctggacgca tcgttggagt ttcaaaagat tcccgcggac gccccgccct gcgactttca
     1321 ctgcaaacgc gcgagcagca tatccgccgc gaaaaagcga ccagcaacat ctgcacggcg
     1381 caggcgctgc ttgcgaacat ggccgctctt tatgcggttt atcacgggcc ggccggtttg
     1441 aaacagatcg caaaacgcgt ccacctcctg accgctacac tctcaaaagg ccttgaacaa
     1501 cttggttatc agatcaagac gaatgctttt ttcgacacgt tgcgcgttga tttgggcaaa
     1561 aagagttccg ctgaaatcat caagctggcc gagtcgcaac ggatcaattt gcggacaatt
     1621 gatgcgaaca caatcggcat ctcgctcgat gaagcg
//
LOCUS       DASZMM010000405         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5394206, whole genome shotgun sequence.
ACCESSION   DASZMM010000405 DASZMM010000000
VERSION     DASZMM010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5394206"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VG754_09770"
     CDS             <1..1044
                     /locus_tag="VG754_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HWF19547.1"
                     /translation="GTGTLIQSGPGPLTLSDTNTFTGGMLITNGSTLAVSGAGSLGAA
                     GTSNNYIGNITNFGTFNYASSAAETFSGIVTGTGALNETGPGELTLSGTDSYTGATSI
                     SSNATLALASSGSINNTASINLAAGGTFDVSAFGLSYSLGGSTTLRPAGTGTNAGSAA
                     ATIVGSSGGTINVGSLVLTFKPQAFSGDATHPVLYVSSGALNLSGSGLTVSNAAATPL
                     GAGTYNLIQVASQNLSVAGTNVTVTGTGLAAGAAASLAVNGGNLNLVVVNTSVPVPVI
                     NSVVVSGNCLIFSGTNGPNNQTYEVITSTNLSTPLHLWTPIQTNSFSGTGTFSVTNSI
                     ATTNQLFFGIKIH"
     gene            1172..1558
                     /locus_tag="VG754_09775"
     CDS             1172..1558
                     /locus_tag="VG754_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19548.1"
                     /translation="MKTITRSLYVKIAAIGLALSFGFSAYAAESPREEVAHAFRLLQQ
                     ANSNYDGHRAKAVSEVEAAGREMNMGLGGEIPEAERQWKSDAQLKEARHLLNDASQKL
                     ESRDRDRASQHLQRAIEEIDQALKTR"
     gene            1740..2381
                     /locus_tag="VG754_09780"
     CDS             1740..2381
                     /locus_tag="VG754_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19549.1"
                     /translation="MACVVTTRADVLELTNGDHYRGNVLSMNANSVEFQSEVLGRIVL
                     PRSKVATVTLHEVIAKPAANTKTTAMPYGSPIVVAATNASVGVSTAVVPGSQADTVVR
                     ELRKQGVDPKLIDQVQEQIFGKSSPEAAQKFNELMGGLLSGQVSVGDIRKQAQRSIND
                     IKAAKKELGPDAGEMLDSYLGILEKFVQEAGADSSSSTPVIPPSPSPQAAPLK"
BASE COUNT          575 a          658 c          680 g          528 t
ORIGIN      
        1 ggaacaggca cattgatcca aagcggtccc ggcccgctca cattgtcgga caccaatact
       61 tttaccggcg gcatgttgat cacaaacggc agcacgttgg cggtcagcgg cgcgggttcg
      121 ttgggcgcag ccggcacgag caacaattat atcggcaaca tcacaaattt tgggacgttc
      181 aattacgcca gctctgcggc ggaaactttc tcgggaatcg tgaccggcac aggcgcgctg
      241 aacgaaaccg gtcccggcga attgacgttg agtggaaccg atagttatac cggcgcgaca
      301 agcataagtt ccaacgcaac gctggcgctg gcaagcagtg gttccatcaa taacaccgcc
      361 tcaatcaatc tggctgcggg cgggacattt gacgtgtcgg cttttggcct ttcttattct
      421 ttgggcggca gcactacgtt aaggcccgct ggaacgggga cgaatgcggg cagcgctgcc
      481 gcgacaatcg ttggaagctc cggcggcacc atcaatgtgg gttcgcttgt gttgaccttc
      541 aaaccccagg ctttcagcgg tgatgccacc catccggtgc tttatgtttc gtccggcgcg
      601 ttgaatctca gcggaagcgg actcaccgtc agcaatgcgg cggcgacgcc tttgggggcc
      661 ggcacttaca acttgattca agtggccagc cagaacctga gcgttgccgg gacgaatgtc
      721 accgtcaccg ggactggtct cgcggcgggc gctgcggctt cccttgccgt caatggcggc
      781 aacttgaatt tggttgtggt caatacgtcc gtgccggtgc ctgtcatcaa cagcgtggtg
      841 gtttccggaa attgtctgat cttcagcggc acgaacggtc caaacaacca gacctacgag
      901 gtgataacct cgacgaacct gtccacacct ttgcaccttt ggacgcccat ccagaccaac
      961 agttttagcg ggacgggtac tttcagcgtg accaattcca tcgccacgac caaccagttg
     1021 ttcttcggca tcaaaattca ttgacctaaa accgtgaacg tcacgcattc gattcgcgtt
     1081 actgtcttcc attgtgattg agcaggcttt taccccatgc ggcagtgatt gacgctgctg
     1141 ttgcgaggta agaagttgtt ttatcaaaat tatgaaaaca ataacacgca gtttgtatgt
     1201 caaaatcgcg gcgattgggc tggccctttc ttttggattc agcgcttacg ccgccgaatc
     1261 gcctcgcgaa gaggttgctc acgcttttcg cctgctgcag caggccaaca gtaattatga
     1321 tgggcatcga gccaaggcgg tatcagaagt cgaagccgcc ggaagggaaa tgaacatggg
     1381 actcggaggc gaaattccag aggcggagcg gcaatggaaa tccgacgcgc aattgaagga
     1441 agcccgtcac ttgctgaatg acgcgagcca gaaacttgaa tcgcgtgacc gcgatcgcgc
     1501 ctcgcaacac ctgcaaagag cgatcgagga aatcgaccag gccctgaaaa cccggtaaac
     1561 cgatttaaac ataagctaat cgcgcagggg cggggttgaa tcctcgcccc tttcgtattg
     1621 atttttacca ggtttctgcc agattgaatc agccaaacag gtggtggatg atgaccaatg
     1681 ttgctcgtat gaaactctcc agtgctgcaa aaacttttcc tttgatcacg atatttgtta
     1741 tggcatgtgt cgtcacaacg cgagccgacg tgcttgaatt gacgaatgga gatcattatc
     1801 gcggaaacgt gctctcgatg aatgcgaaca gcgtcgagtt ccagagcgag gtcttgggac
     1861 gcatcgtcct cccgcgcagc aaagtcgcga cggtcacatt gcatgaggtg attgccaaac
     1921 cggctgcaaa cacgaagacg accgcgatgc cgtatggttc gcccatcgtg gttgcggcca
     1981 caaacgccag tgttggcgtc tccactgcgg ttgtgccggg cagccaggcg gacactgtgg
     2041 tgcgggaatt acgcaagcag ggcgtggatc ccaagttgat agaccaggtg caggagcaga
     2101 ttttcggcaa atccagcccg gaagcggcgc aaaagttcaa cgaactcatg ggcggattgc
     2161 tcagcggcca ggtatcggtt ggagacatcc gaaaacaggc ccaaagatcc atcaatgata
     2221 tcaaggcggc caagaaagaa ctcgggcctg acgcagggga aatgttggac agttatctcg
     2281 gcattttgga aaaattcgtg caggaagccg gcgcagactc cagttcctcc actcctgtta
     2341 ttcctccatc accttcacct caggccgctc ctctaaaata aaacggctaa gtcggtgagt
     2401 gggtgtttac cgacagctaa aatctcttcc gtccaacgtg c
//
LOCUS       DASZMM010000406         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_462376, whole genome shotgun sequence.
ACCESSION   DASZMM010000406 DASZMM010000000
VERSION     DASZMM010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462376"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2388)
                     /gene="metH"
                     /locus_tag="VG754_09785"
     CDS             complement(<1..2388)
                     /gene="metH"
                     /locus_tag="VG754_09785"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWF19550.1"
                     /translation="MNQDISHQANRTEQLERLLRERIVIIDGAMGTMIQTYKLDEAAF
                     RGERFKDWHKDLKGNNDVLNITRPGVIEEIHRQYLEAGADIVETNTFNSQAISLADYK
                     MEALAYELSKTGAECARRAADKVTAAQPGRICFVAGAIGPTTRTSSISTDVNNSAARG
                     TTYDELVAAYYDHARGLVDGGADILLVETIFDTLNSKAAFFAIEKLFEDRRIRLPIMA
                     SVTFIQKGSNRGVTGQTVEAFWNSISHVPLLSVGMNCALGPQEMRPLIEELSQIAPIY
                     VSCYPNAGLPNPLLPTGFPETPETLAPQLQEWAQNGWLNVVGGCCGTTPPHIKKIAEA
                     FHGLPRRVPPRVEPYTRLSGLEALTLRPDTNFVNVGERANVTGSPKFAKLILAGQYEE
                     ALAVAKQQVENGAQVIDINMDEGMLDSEKAMVHFLNLIASEPDIARVPIMIDSSKWSV
                     IEAGLKCIQGKGVVNSISLKEGEARFIEQARLVKRYGAAVVVMAFDESGQADTMERKV
                     EICTRSYKILTEKVGLQPQDIIFDPNILTVATGMEEHNNYAVNFIEATRRIKATLPHC
                     KVSGGISNISFSFRGNNVVREAMHSAFLYHAIKAGMDMGIVNAGMLEVYEEIPKDLLE
                     LVEDVLLNRRPDATERLVKFADSVKQKGKVEVVEDEWRKGTVEERLSHALVKGIVDYI
                     DQDTEEARQKYVKPLLVIEGPLMSGMNVVGDLFGSGKMFLPQVVKSARVMKKSVAYLL
                     PYMEEEKRKSGGGQPQGRILMATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPC"
     gene            complement(2768..3343)
                     /gene="def"
                     /locus_tag="VG754_09790"
     CDS             complement(2768..3343)
                     /gene="def"
                     /locus_tag="VG754_09790"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWF19551.1"
                     /translation="MILEVVKFGHPTLRKKGARVETITPALKELIENMFETMYATRGI
                     GLAAQQVAEALQVTVLDVREASAERPSTLELNGEPADVHAFMPLVLINPEIKPVGKPV
                     TGPEGCLSFPEIFADITRPESVEVIALNEKGKRIQFRCGGLLARAVQHEADRLNGLLF
                     IDRMDADTKQDLKPELDALQTATKAALKKKK"
     gene            complement(3385..>3573)
                     /locus_tag="VG754_09795"
     CDS             complement(3385..>3573)
                     /locus_tag="VG754_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19552.1"
                     /translation="PLITTGAGDHFNSGFCLGKLLGFENDLCVLTGVATSGHYVRTGH
                     SPAITDLAAMMRNWPAQN"
BASE COUNT          731 a         1020 c          989 g          833 t
ORIGIN      
        1 gcaaggcacc attactccga gatcgatgac ctcgtagtta ttgcagccaa gcaccacgcc
       61 gacaatattc ttgccgatgt cgtggacgtc gccttttacg gtggccatga gaattcgacc
      121 ctgcggctgg ccgccgccgg acttgcgctt ttcctcctcc atgtacggca gcagatacgc
      181 cacggatttc ttcatcacgc gcgcgctctt gacgacttgg ggcaaaaaca tcttgcccga
      241 gccgaacaaa tcgcccacca cattcatgcc gctcatcagc gggccttcga tgaccaggag
      301 cggcttgacg tatttttggc gggcttcttc ggtgtcctgg tcaatgtaat cgacgatccc
      361 tttaaccagt gcgtggctga ggcgttcctc aactgtgcct ttgcgccact cgtcttccac
      421 gacttcaacc ttgccttttt gtttgaccga atcggcaaat ttgacaagtc gctcggtggc
      481 gtccgggcgg cggttaagta aaacgtcctc gaccaattcc agaaggtcct tgggaatttc
      541 ctcgtacact tccagcatgc cggcgttcac gatgcccata tccatgcccg ccttgatggc
      601 gtgataaagg aacgccgagt gcatggcttc acggacaaca ttgttgcccc ggaaggaaaa
      661 ggaaatgttg ctgattccgc cgctgacctt gcaatggggc agggtggcct tgatgcgcct
      721 cgtggcctca ataaaattca ccgcataatt attgtgctcc tccatcccgg tggcgacggt
      781 caggatgttg ggatcaaaaa taatgtcctg cggttgcagg ccgacttttt ccgtcagaat
      841 tttataggaa cgggtgcaaa tttcaacttt gcgctccatg gtgtccgcct ggcccgattc
      901 atcaaaggcc atcacgacca cggcggcgcc atagcgtttc acgaggcgcg cctgttcgat
      961 gaatctggcc tccccctcct tgagactgat ggaattgacg acgcctttgc cctgaatgca
     1021 cttgaggccc gcttcgatca cagaccattt ggagctgtca atcatgatcg gcacgcgggc
     1081 gatgtcgggt tccgaagcga tgaggttcag gaaatgaacc atggcttttt cggaatcgag
     1141 catgccttcg tccatgttga tgtcgatgac ctgcgcgccg ttctcaactt gttgcttcgc
     1201 cacagccagc gcttcctcgt actggccggc cagaatcaac ttggcaaact tgggcgaccc
     1261 ggtgacgttc gcccgttcac cgacgttgac gaaattcgtg tccggccgga gcgtgagtgc
     1321 ttccaaaccg ctgaggcgcg tgtagggctc aacgcgcggt ggaacgcgcc ggggcaggcc
     1381 gtgaaaggct tcggcaatct tcttgatgtg cggtggagtg gttccgcaac agccgccgac
     1441 gacattcaac cagccgtttt gggcccactc ctgcaactgt ggcgcaagtg tttccggcgt
     1501 ttcgggaaac ccggttggca acagcggatt gggcaggccg gcgttgggat agcagctcac
     1561 atagatgggc gcaatctgtg acagttcctc gatcagcggc ctcatttcct gtggtccaag
     1621 cgcacagttc atgcccacgc tcaacagcgg gacgtgcgat atcgaattcc agaaagcctc
     1681 caccgtttga ccggtgacac cccggttgct gcccttttgg atgaacgtca ccgaagccat
     1741 gatcggcagt cggatgcggc ggtcttcaaa tagcttttcg atggcgaaga aggcggcctt
     1801 tgaattcaac gtatcaaaga tcgtttcaac gagcaggata tcggcgcccc cgtccaccag
     1861 tccgcgggcg tggtcgtaat acgcggcgac cagttcatcg tacgtcgtgc cgcgtgcggc
     1921 ggagttattg acatccgttg agatggaaga ggtgcgcgtg gttggcccga tggctccggc
     1981 gacaaaacag atccggccgg gctgtgccgc cgtgactttg tcagccgcgc gacgggcgca
     2041 ttccgctccc gttttggaga gctcgtaagc cagcgcctcc atcttgtagt cggccaggga
     2101 gatggcctgg gaattgaacg tgttcgtttc cacgatatcc gctcccgcct cgagatattg
     2161 gcgatggatt tcctcgatga ccccgggccg cgtgatgttc aagacatcat tattgccctt
     2221 caggtctttg tgccaatcct tgaaccgctc gccgcgaaat gccgcctcgt ccagtttgta
     2281 ggtctggatc atggtgccca tggcgccatc gatgatcacg atgcgttcgc gcaaaagacg
     2341 ctcaagttgt tctgtccgat tggcttgatg agaaatgtcc tgattcacgg tcagtaatca
     2401 taacccgttc agggcgcgtt gcaagtgaaa taatcaatgc atcttgatat gaagatatga
     2461 tatgtatgcc ccttcgcagt cacaaaaaat aaaatccaag aagtatatct taacgtgtat
     2521 ctacgtgccc attctggcca ccacttcgct gacttttctt gttactcgtg cgaagtgccg
     2581 cgccgaagct cccagcgact tgtcccgcca cagctctcct tgagcgacag cggaaggcgg
     2641 gctcgcaaaa actccggaaa gcgcatcgta aacaggcgtg gagggcacag agaatggtac
     2701 tattccgggt ggggacctca acgggatggg aagcgggaaa cggaggccgt tggaattgca
     2761 atcccggcta tttctttttc ttcaatgccg ctttggtggc ggtctggagg gcgtccaact
     2821 cgggtttcaa gtcttgcttc gtgtctgcgt ccatgcggtc aatgaacaac aggccgttca
     2881 aacgatccgc ttcatgttgc accgcccgag caagcagccc gccgcagcga aattgaatcc
     2941 gcttcccttt ttcgttcagg gcgatcacct ccacggactc gggccgggtg atgtccgcga
     3001 aaatttctgg aaaactaagg cagccttccg gccctgtgac cggttttccc acgggcttga
     3061 tttccgggtt gatcagcacc aaaggcatga acgcatgaac gtccgccggt tcgccattca
     3121 gttcaagcgt cgatggacgc tccgcagagg cttcgcgcac gtcaagcacc gtcacctgca
     3181 gggcctcggc gacttgttgg gcggccaagc cgatgcctcg cgtggcgtac atcgtttcaa
     3241 acatgttctc gatcaattcc ttgagggcag gggtgatcgt ttcgactcgc gcgccttttt
     3301 tgcgcagcgt tggatggcca aattttacaa cttccagaat cattccctca aaatattatt
     3361 cgatacggtt ccaggggcaa ccggctaatt ttgcgccggc caattccgca tcatggcagc
     3421 caaatcagta atggccgggc tgtggccggt gcggacatag tgcccgctgg tggctactcc
     3481 cgtcagcacg caaagatcat tttcaaaacc gagcaatttg ccaaggcaga acccggaatt
     3541 gaaatgatca cccgcgccgg tggtgatcaa cgg
//
LOCUS       DASZMM010000407         6026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_981220, whole genome shotgun sequence.
ACCESSION   DASZMM010000407 DASZMM010000000
VERSION     DASZMM010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981220"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            668..1543
                     /locus_tag="VG754_09800"
     CDS             668..1543
                     /locus_tag="VG754_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWF19553.1"
                     /translation="MNIHHLELFYYVARHGGISEAVRNIPYGIQQPAISGQIAQLEEF
                     LGTTLFHRRPFALTPAGEKLYKFIEPFFSGVEAMTAELQGGTTHQLRIGSSDIVLRDH
                     LPEVLQAVRKKFPQLKVTLREGYQPELEDMLERQEIDMAVTVTERKPIAGVRSLILLE
                     LPLILVVPVNSPITAAEQLWKRDKIHEPLICLPATEVMSRNFQQGLSKLGVDWFPSIE
                     INSTDCIESYVANGFGIGLTVSIPKTKMSPKVRKVELPGFGPLLVGALWRGKPSPPLR
                     TLLDELEARALRLKE"
     gene            1625..2692
                     /gene="pilM"
                     /locus_tag="VG754_09805"
     CDS             1625..2692
                     /gene="pilM"
                     /locus_tag="VG754_09805"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HWF19554.1"
                     /translation="MPLPFLNGPTKKRDQIIAVDLGARSTKAVHLQRRGDVSTLLRYC
                     VMDAPIYEKSLSVDLLADHLKAICLALEAKSKFVSLALSINDSIVRQTELPPIPVDEM
                     RQILKNNSKTYLQQDLPGHVFDCYFINRTNGAVEPSKPASGPQKAKVLVGGAKKQLTD
                     DLQAAVKQAGLIPDQVIPGIVCPSNAFELAMPEIFKGDAVALVDIGFKNTSICLLQHG
                     DLIVSRVVNIGGDKLTQGLSEAMNISYAEAEGIKVGMPAEVQSNLEPLLMPLGRELRA
                     SIDFFEHQQDRTISQVYISGGSARSEFIMKTLETELMVPCKSWSPVGNVQLGLPPAQA
                     SEIEQIGPQLTIAMGTAAAAF"
     gene            2701..3387
                     /locus_tag="VG754_09810"
     CDS             2701..3387
                     /locus_tag="VG754_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19555.1"
                     /translation="MPIRINLLAEAQAAEELRRRDPVKRALWAGVCLILMVVVWISSL
                     QVKIMADTSRLGNLQARLDSRTNQYTQILQNKQTLLDINEKLAALNRLAAERLLQATL
                     LDAPLHTPVDGIQILRLHTEQGFDVTPAVPAVVERGKTVVPAKSAGSIERVKLILDGK
                     DTSPNPGNEQITKFKETLAGTPYFHAQQISTNNILLKNLSAPQLDSETGKPYVLFSLE
                     CQYPERAHSL"
     gene            3384..3971
                     /locus_tag="VG754_09815"
     CDS             3384..3971
                     /locus_tag="VG754_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19556.1"
                     /translation="MKKLSKEKRLHLILVAMGTAAVIAGLWFGVISMQKNKLRDIARK
                     SEGVQHEMDKIQKVVTAANELQLSLTASTNRLDGIEAGIPSASGDLFAWIVNSLKQFN
                     VPSYKVDMPQIGSPGVGEVRMFQNFPYNQASVSVSGSAYYYDFGKFVADLENHFPYLR
                     IGNLDLEPGMGTTPEEHEKLSFHMDIITLVKPVAH"
     gene            3984..4460
                     /locus_tag="VG754_09820"
     CDS             3984..4460
                     /locus_tag="VG754_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19557.1"
                     /translation="MKFSRLFPIGLKVVITLLCLAVLPAARATNTTNAPSSGAKTNSA
                     PEETPIPLSVFDLSVKPTRDPFFPLSNRQPVPALATNAPAISASTFTLKGLSGSFSHP
                     LALVNNRTVAPGEDAEVTTPTGKFRIHCVEIKPPSVFLRISSQSDLIEISLRKTAQ"
     gene            4799..>6026
                     /locus_tag="VG754_09825"
     CDS             4799..>6026
                     /locus_tag="VG754_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWF19558.1"
                     /translation="MKLNAALFSLVALTATCTITVAETYAPVPGPSQPLTTLAATSTN
                     AATPTAQADAGAATNAPVAPAAASTNAPVADAGTNAPAAIPAAAADAATNAPAANPPP
                     APVAATNAPAPTAAVPTVPAAPVQVAAAADATTPAAAAIPATPADTSSNSPGPGAIIP
                     LIVMDEVPLTDAIKNLARQAGLNYMLDPKINYGAPDATGVVHPQPTVSLRWENLTADQ
                     ALNAVLANYNLIMMDDPKTHIARITIKDPAAPDPLVTKIVQLKFCDASNMVENVQTVL
                     LDKRSKVIADSRTSQLVVVATEQELNAADQLIARLDTPTKEVLIEAKLVELQKNPSTS
                     KGIDWSGTLSAQKVTFGNNVLGGASTVPYGTTFSQTPIQGLVNGTLTNIGAVSQITYT
                     ANNTVQNPGLLVDTAHG"
BASE COUNT         1411 a         1752 c         1517 g         1346 t
ORIGIN      
        1 ctcgttgtcg ttggaaggag gaaggctctt tgacatccta ataagttcaa agttaacctg
       61 acgccgagtc gtggccgtct ggaacggacc ttcatgcatc cagcataacc gaccgagttt
      121 tcggttcaaa attgctgaaa agcgcaatct gccacagttg gtttcagatc atttcacctt
      181 gtttcagtta ttttcagata aaccgggata aacgggataa aaaaataaat atttttttgc
      241 gcgagagaag gcttgcgttt tcgcggcgcc actgtgtcgg ttaatgtcgc ttggcgtcgg
      301 tttggcaagt agggatggcg cgctgcgccg tccagacatc gcagcgcgat gtctctacca
      361 tctgaaaact cacgttttca gctacggtgc acagagcgtt caagttgtaa gtggttgtaa
      421 acccgcttaa acccgcatag cttcacctaa acccgccaaa aatatagggg gggtggactg
      481 aacatcgaac gcccaacatc gaatgtcgaa cgctgaaatg ggaaaaagcg cttaacgcct
      541 gtagtagtaa cattcgatgt tcagagttca gcgttggttg ttcgatgttt tctctgctgt
      601 gtcgcttggc gcggcgcatc ggttcttgcc tcgaatgggt taactgataa aacggtgcaa
      661 tagttgtatg aacattcatc atctggagct tttttattac gtggcccggc atggaggcat
      721 cagtgaagcg gtgcgcaaca tcccttacgg catccagcag ccggccatca gcggccagat
      781 cgcgcaactg gaggaatttc tcggcacgac gctttttcat cggcgtccgt tcgcgctcac
      841 gccagccggt gagaagctct acaaattcat cgagccgttc ttttccggcg tcgaggcgat
      901 gaccgctgaa ttgcagggcg gcacgacaca tcagttgcgc atcggctcct cggacatcgt
      961 gctgcgcgat catctgccgg aagtgctcca ggcggtgcgg aagaaatttc cgcaactcaa
     1021 ggtcactttg cgcgaaggtt atcaaccgga actcgaagac atgttggaac ggcaggaaat
     1081 tgacatggcg gtgaccgtca ccgagcgcaa gccgattgcg ggagttcgtt ctttgatatt
     1141 gctggaactg ccactcatcc tagtcgtgcc ggtgaacagt cccatcaccg ccgccgagca
     1201 attgtggaag cgggacaaga tccatgagcc gttgatctgc ctgccggcga ctgaggtgat
     1261 gtcgcgcaat tttcagcaag gcttgagcaa attgggagtg gattggttcc caagcatcga
     1321 aatcaattcg acggattgca tcgaaagcta tgtcgccaat gggtttggaa ttggactgac
     1381 ggtgtctatt cccaagacaa agatgtctcc aaaagtccgc aaggtggagt tgccagggtt
     1441 tgggccgttg ctggtcgggg cgctttggag gggcaagcct tcgcccccgt tgcggacact
     1501 tttggacgaa ttagaggcac gggcgctgcg tttaaaggaa taaatcgcgc cgccgacgag
     1561 gttggagcgg aaaatggcaa aagagttgct acttaccggg cgtacgtagt gacaaattaa
     1621 acttatgccg ctgccattct taaacggccc gacgaagaaa cgcgaccaga tcatcgcagt
     1681 cgatctgggc gcgcgctcca ccaaggccgt ccatctgcaa cgccggggcg acgtctccac
     1741 gctgttgcgt tattgcgtga tggatgcgcc catctatgag aagtcgctct cggtggacct
     1801 tctcgccgac catctcaaag ccatctgcct ggcgctcgaa gcaaagtcga agttcgtcag
     1861 tctcgcgttg agcattaatg attcaatcgt ccgccagaca gaattgcctc ccattccggt
     1921 ggatgagatg cggcaaattc tcaagaacaa ttccaagact tatcttcaac aggacctgcc
     1981 cggccatgtg ttcgattgct atttcatcaa ccggaccaac ggcgcggttg aaccgtcgaa
     2041 accggccagc ggtccgcaaa aagccaaggt gctcgtcggt ggggcgaaga aacaactcac
     2101 agatgatctt caagcggctg tgaaacaagc cgggctcatt cccgaccagg tgattccggg
     2161 aattgtctgt ccttcgaatg cgtttgaatt ggccatgccg gaaattttca aaggcgacgc
     2221 agtcgccctt gtggacattg gtttcaagaa cacctccatt tgcctgctgc aacatggtga
     2281 tttgatcgtc agccgcgtcg tcaatattgg cggcgacaaa ttaacgcagg gattgtccga
     2341 agcgatgaac atcagctatg ccgaagccga aggcatcaag gtcggaatgc cggcagaggt
     2401 acaatccaac cttgagccgc tgttgatgcc gttgggccgc gagctgcgcg cctcgattga
     2461 tttttttgaa catcagcagg accgcaccat cagccaggtt tacatttccg gcggctccgc
     2521 ccgttcggaa ttcatcatga aaacgctcga aacggaactg atggttccct gcaaatcatg
     2581 gagcccggtc ggcaatgtgc aattgggatt gccgcctgcg caagcttccg aaattgaaca
     2641 aatcggcccg caactcacga tcgcgatggg gactgcagcc gccgcattct aaaacagctt
     2701 atgcctatcc gcatcaattt gctcgccgag gcgcaagccg ccgaggaact tcgccgtcgt
     2761 gacccggtta aacgggccct ctgggcgggc gtctgtctga ttctcatggt cgtagtgtgg
     2821 atcagttcgc tgcaggtcaa aatcatggcc gatacttcca ggctaggcaa tctccaggcc
     2881 cgcttggatt cccgcaccaa tcaatacacc caaattttgc agaacaaaca gacgctcctt
     2941 gacatcaatg aaaagctcgc tgcgctcaac cgcctcgctg ccgagcgttt gctgcaagca
     3001 acgcttctcg atgctccctt gcacaccccg gtggacggga ttcaaattct tcgactccac
     3061 accgagcaag gctttgacgt gactcccgcg gttccggccg tcgttgaacg cggaaaaaca
     3121 gttgttcccg ccaagtcggc cggatccatc gaacgtgtca agttgatcct tgatggcaag
     3181 gacaccagcc ctaatcccgg caacgaacag atcaccaagt tcaaggaaac cctggctggc
     3241 actccgtatt ttcacgccca gcagatttcc acaaacaaca tcctgctcaa aaacctttcc
     3301 gctccgcagc tcgacagtga aaccggcaaa ccgtacgtcc ttttcagcct cgaatgccaa
     3361 tatcctgaac gcgcccacag cttatgaaaa agctctccaa agaaaaacgc cttcatctca
     3421 ttctcgtcgc aatgggcacc gctgcggtca tcgcaggcct ttggtttggg gtgatttcca
     3481 tgcaaaagaa caagctgcgt gacatcgctc ggaaaagcga aggtgtgcaa catgagatgg
     3541 acaagataca gaaagtggtg actgcggcaa acgagctgca actctctttg accgcctcca
     3601 ccaaccgcct cgatggaatc gaagccggca taccttccgc ttcgggcgac ctgttcgcct
     3661 ggattgtcaa ctctctcaag cagttcaacg tgccgagtta caaggtggac atgccgcaga
     3721 tcggctcgcc tggcgtcggt gaggtccgga tgttccaaaa ttttccgtat aatcaagctt
     3781 cggtgtccgt aagcggttcc gcatattatt atgacttcgg aaaattcgtg gccgatctcg
     3841 aaaatcattt tccctatctg cgcatcggga acttggactt ggaaccgggc atgggcacga
     3901 ctccggagga acatgaaaag ctttccttcc acatggacat catcaccctg gtcaaacctg
     3961 ttgcgcacta acccgaatct cccatgaaat tctcacggct tttcccaatc ggcttgaagg
     4021 tggtcatcac cttgttgtgc ctcgctgttt tgcctgccgc gcgcgcaacc aacacgacta
     4081 atgcgccctc aagcggcgct aaaaccaact cggcgcctga ggaaacaccg atcccccttt
     4141 ctgtgtttga cctgagcgtc aaaccgacca gggacccgtt ctttccgctt tcgaatcgcc
     4201 agccggtccc agcgctggca accaacgcgc ccgcaatttc cgcttcgact ttcacgctga
     4261 aaggtctttc cggttcgttc agccatccgc tggcgcttgt caacaatcgc acggtcgcgc
     4321 caggtgaaga cgccgaagtc accactccta ccggaaaatt caggatccac tgcgtggaaa
     4381 tcaagccgcc atctgtcttt cttcgtatct cctcccaatc cgatttgata gaaatctcgt
     4441 tgcgtaaaac cgcccagtga ctcgttgtaa ttcgggattt caactcaaca tctcaaatta
     4501 aggtattact ttgcccatga aaattgtcgt caaatcgaat caatcgcacg ctcattcttg
     4561 cgagacccgc aaagcgaaat gcaccatgtt tcagtccatt tggccttcaa tatatatgga
     4621 tttgaaattc ccgccctgtc ttaatgctgg acaacctttg cgtcagagtg ggcacaaaac
     4681 caataaaatc aaccctttta ccctgctttt ggcttggcat tgcgtctgct gaaacgtctc
     4741 agacgcattg agcggccaaa tccgctgatg caagggtcga actaactaaa aaaataaaat
     4801 gaaactcaac gctgccttat tcagcctcgt cgcgctgacc gccacttgca ccatcactgt
     4861 ggccgagacc tatgcaccgg tccctggtcc aagccaacca ctcaccactc tcgccgcgac
     4921 atccaccaat gccgcgacgc cgacggcaca agcggatgct ggcgcggcaa caaatgctcc
     4981 agtggcacct gctgctgcca gcaccaatgc tcccgttgct gatgccggca cgaatgcccc
     5041 ggcggctatt cctgctgccg ctgctgatgc cgccacaaat gcgcctgcgg caaacccgcc
     5101 ccctgccccc gtggccgcta ctaatgcacc ggccccgacc gccgcagttc caacagttcc
     5161 cgctgcgcca gtccaggttg ccgccgctgc ggatgcgaca actccagccg ctgctgcaat
     5221 tcccgccacg ccggccgaca cctcctccaa ttctcctggc cccggcgcaa tcatcccgct
     5281 catcgtgatg gatgaagtgc cgttgaccga cgccatcaag aatctcgcgc gccaagctgg
     5341 cttgaattac atgcttgatc cgaagatcaa ttatggcgct ccggatgcca ccggtgtggt
     5401 gcatcctcag cccactgtct ccttgcgctg ggaaaatctt acggccgacc aagcgttgaa
     5461 cgcggtgctc gccaattaca atctcatcat gatggacgat cccaagaccc atatcgcccg
     5521 catcaccatc aaggaccctg ccgcacccga tccgctcgtg accaagatcg tccaattgaa
     5581 attctgcgac gcctccaaca tggtcgaaaa tgtccagacc gttctccttg acaaacgcag
     5641 caaggtcatt gcggattcgc gcacaagtca gttggtcgtc gttgccaccg agcaggaact
     5701 gaatgccgct gatcaattga tcgcccgcct cgacactccg accaaggaag tgctgattga
     5761 agccaagctg gttgaattgc agaaaaaccc ttccacctcc aagggtattg actggtccgg
     5821 gacgctgtcg gcccagaaag tcactttcgg aaacaatgtt ttgggtggag cgagcacagt
     5881 tccctacggc acaaccttca gtcagacccc aattcaaggg cttgtgaacg gcaccttgac
     5941 taacattggc gctgtcagtc aaattacgta cacggcgaac aatactgttc aaaatcccgg
     6001 cttgctggtg gatacagcgc acggtt
//
LOCUS       DASZMM010000408         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_981223, whole genome shotgun sequence.
ACCESSION   DASZMM010000408 DASZMM010000000
VERSION     DASZMM010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981223"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VG754_09830"
     CDS             complement(<1..1328)
                     /locus_tag="VG754_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWF19559.1"
                     /translation="MPDEKNDNSLPVSNTPLFAANEKITKVNVADEIKNSFLDYSMSV
                     IISRALPDVRDGLKPSQRRILYAMNELGVAPTRKHLKCARIVGETMGKYHPHGDQAIY
                     PTLVHMAQPWAMREVLIDGQGNFGSVEGDPPASMRYTEARLAHLGAVLMDDMEKDTVD
                     FVPNYDEEHVEPVVLPAAFPNLLVNGGTGIAVGMATNMAPHNLGEVVDGICAQIDNPN
                     ISLAELMKHVKGPDFPTGCTICGLEPIKKYFTDGRGSIKVRGKVGLEEMKGGREQIII
                     TAIPYNVNRAVLVERIAELVNDKTLPEITAVRDESDENTRVVIEIRRDAVPKVVINNL
                     YKFTALESSFAVNALAIDHGRPKTLSLKDIIACYIEHRREVVLRRTRFELRKAEDRAE
                     QLEGYLIALANMDEFIRIIRGSSNREEAKIKLLAFEFSRGQVEQLGILIR"
     gene            complement(1460..>2967)
                     /locus_tag="VG754_09835"
     CDS             complement(1460..>2967)
                     /locus_tag="VG754_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWF19560.1"
                     /translation="EGLVAVIAIKHPDPKFNNQPKEKLLSTEVEGIVSSITYEGLMSH
                     FDRNPPVAKRIVDKGLNAARAREAARKAREAVRKSALTGGGLPGKLADCSDRDPANTE
                     MYIVEGDSAGGSAKQGRDRKFQAILPIRGKLINVEKARLDKVLQNNEIRTMITAVGTG
                     IGDGDGEGAFNIERLRYHKIIIMTDADVDGSHIRTLLLTFFYRQMPELIKRGFVYIAQ
                     PPLYQITRKKRVEYVDDDAALNKILIQIGTEEVRLRNLSDNKELTQNQLAEILELLES
                     LDKYAKALRRHGGDFSDFIEHRNAGTGELPRHLVKVRAGNDESVHYFVAEEDMAKFSA
                     DNADLRLSGEEEGDTDIIEKKNGPSRRARHVELHESKAIGELLAKLSRKGLNVEHYSA
                     QDKPLFELIEGEGEKQTVKPLFSIPEILESVIDVGKQGLQIKRFKGLGEMNPGQLFET
                     TMDPARRKLLRVDLTDAVEAEEMFTKLMGDEVEPRKQFIEDNALNVRNLDV"
BASE COUNT          602 a          865 c          783 g          717 t
ORIGIN      
        1 ctgcggatca aaatgcccaa ctgctccacc tgtccccggg aaaattcgaa agccagcagc
       61 ttgatcttcg cctcttcgcg attcgaggaa ccgcggatga tgcggatgaa ttcatccatg
      121 ttcgcgagcg cgatcaaata accttcgagc tgctcggcgc ggtcttcggc cttgcgcaat
      181 tcaaagcgcg tgcggcgcaa caccacttcg cggcgatgct cgatgtaaca ggcgatgatg
      241 tctttgagcg agagagtttt gggccggcca tgatcaatcg ccagcgcgtt caccgcgaag
      301 ctcgattcga gcgcggtgaa tttataaaga ttgttgatga caaccttcgg tacggcatcg
      361 cgccgaattt ctatgaccac gcgggtgttt tcgtcggatt cgtcgcggac ggcggtgatt
      421 tcaggaagtg tcttatcgtt gaccaattcg gcgatgcgct cgacaaggac ggcgcgattg
      481 acgttgtagg gaatcgcggt gatgatgatc tgctcgcgtc cgcccttcat ctcctcgagg
      541 ccgaccttcc cgcggacttt gatgctgcca cgcccgtccg taaaatattt tttgatcggc
      601 tcaagcccgc agatggtgca accggtcgga aaatccgggc ctttgacgtg cttcatcaac
      661 tccgcaaggc tgatgttcgg attgtcaatc tgcgcgcaaa tcccgtccac cacttcgccg
      721 agattatgcg gcgccatgtt ggtcgccatg ccgacggcaa tgcctgtgcc gccgttgacg
      781 aggagatttg gaaatgcggc aggaagaaca accggctcaa catgttcctc gtcataattt
      841 ggaacaaaat ccaccgtatc cttctccatg tcatccatca ggacggctcc aagatgcgcc
      901 aaccgcgctt cggtataacg catggacgcc ggcgggtcgc cttccacgga accaaagttc
      961 ccctgcccgt ctatcaggac ttcgcgcatt gcccaaggct gcgccatgtg cacgagcgtc
     1021 gggtaaatcg cctgatcgcc gtgtggatgg tacttgccca tcgtttcacc aacaatcctg
     1081 gcgcatttga gatgtttgcg agtaggcgca acacccagct cattcatcgc ataaagaatg
     1141 cgccgctgtg aaggtttcaa tccgtcgcgc acgtctggca acgcgcggga aatgatgacg
     1201 gacatcgaat aatcgaggaa cgaattcttg atctcgtccg cgacgtttac cttcgtgatt
     1261 ttttcgttgg ccgcgaacaa cggagtgttg ctcaccggca ggctgttgtc gtttttttcg
     1321 tcaggcatgg caaaaaattc aaaatgtaaa attcaaaatt caaaattaaa aattaaaaac
     1381 gtcttcaaaa ccacagatgt ttggtgtcca tgctttagcg tgttccgcat cggacagcct
     1441 gaaggctgga caccgaacat caaacatcca ggttgcgcac gttcagggcg ttgtcttcga
     1501 tgaattgctt gcgcggctcg acttcgtcgc ccatcagctt ggtgaacatt tcctcggctt
     1561 caacggcatc ggtcaggtcc acgcgcagga gtttgcgcct ggccgggtcc atcgtcgttt
     1621 caaacaactg gcccgggttc atttcgccca agcccttgaa ccgcttgatc tgcaagccct
     1681 gcttgccaac atcgatgacg ctttccaaaa tttccgggat ggaaaacagc ggcttgaccg
     1741 tttgtttttc cccttcgcct tcgatgagtt caaacaacgg cttgtcctgc gccgagtaat
     1801 gttccacgtt caatcccttg cggctcaatt tcgccagcaa ttcgccgatg gccttgctct
     1861 cgtgcaattc cacatgccgc gcgcgccgtg acggaccgtt ctttttctcg atgatgtcgg
     1921 tgtcgccttc ttcctcgccc gagagccgca aatcggcgtt gtccgcgctg aactttgcca
     1981 tgtcttcctc ggcgacgaag taatgcaccg attcgtcatt gccagcgcgc accttgacca
     2041 agtgccgggg caactcgccc gttcccgcgt tccgatgctc gatgaaatcg ctgaaatcac
     2101 cgccatgccg gcgcaaggcc ttcgcatatt tgtcgagtga ttcgagcaat tccagaattt
     2161 cggctagctg gttttgcgtg agttctttgt tgtcggaaag attccgcaag cggacttcct
     2221 ccgtgccgat ctggatcaaa atcttgttca acgcggcatc gtcatcgaca tattcaacgc
     2281 gtttcttgcg cgtgatctgg tacaacggcg gctgcgcgat atacacgaag ccgcgtttga
     2341 tcaactccgg catctgccgg taaaaaaacg tgagcaacag cgtgcggatg tgcgaaccgt
     2401 ccacatcggc atcggtcatg atgatgattt tgtggtaacg caaccgctcg atgttgaaag
     2461 cgccctcgcc gtcgccatcg ccgatgccgg tacccaccgc ggtgatcatg gtgcggattt
     2521 cgttgttttg aagcaccttg tccaaacgcg ccttctccac gttgataagt ttcccgcgaa
     2581 tgggcaaaat cgcctggaac ttgcggtcgc gcccctgctt cgccgagccg cctgcggaat
     2641 caccctcgac gatgtacatc tcggtgttcg ccgggtcgcg gtcggaacaa tcggccagct
     2701 tgccgggcaa accgccgccg gtaagagcgc ttttgcgcac ggcttcgcgg gctttccgcg
     2761 ccgcctcgcg cgcgcgcgcc gcattcagac ccttgtccac gatccgtttt gcaacgggcg
     2821 ggttccggtc gaaatgcgac atcaaccctt catacgtgat ggaactgacg atgccttcga
     2881 cttccgtcga aagcagtttt tccttgggct gattgttgaa tttcgggtcg ggatgcttga
     2941 tggcaatcac cgcgaccaat ccttcca
//
LOCUS       DASZMM010000409         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4842949, whole genome shotgun sequence.
ACCESSION   DASZMM010000409 DASZMM010000000
VERSION     DASZMM010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4842949"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VG754_09840"
     CDS             <1..1226
                     /locus_tag="VG754_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWF19561.1"
                     /translation="RARGAHVWDAEGRKYLDLTAAFGVAAAGHANPRVVRAGQKQMQN
                     LLHAMGDVHPHALKAKLARELSRLTFERWSAAKFKVQGSKFKVGKTIFCNSGFEAVEA
                     ALKTAMLATSKPGVIAFEGAYHGLGYGTLNATHREHFRGSFMKQIGGFAEFVGFPTTP
                     RQMKRSLSEIRDLLHYKEIGAVLVEPVQARGGINVPPQTFLPALRKLCDEYAAVLVLD
                     EIYTGFGRTGKWFACEHSKTMPDIICLGKALTGGFPLSACVGRADLMDAAWPVSGGEA
                     IHTSTFLGHPVGCAMALAQIEEIRAKKLVQRSAQLGQYLLRELKKNISHPQFDISIRG
                     LGLMAGVELCLHNRVPATIVSLRIIKEMLHRGFILLPEGSHANVISFTPPLTITRAQL
                     TAAVNALGAALSSPE"
     gene            1345..>1588
                     /locus_tag="VG754_09845"
     CDS             1345..>1588
                     /locus_tag="VG754_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HWF19562.1"
                     /translation="MKQVLESGRIQLTKSLGQNFLHDGNQLRCIVDAAELKKTDRVLE
                     VGPGLGPLTELLIEKAGEVLAIEKDRRLFEFLQQRFV"
BASE COUNT          350 a          420 c          466 g          352 t
ORIGIN      
        1 aacgcgctcg cggcgcgcac gtctgggacg cggaaggtcg caagtatctg gatctgacgg
       61 cggctttcgg cgtcgcggct gcgggccatg ccaatccgcg ggtcgtgcga gccggccaga
      121 aacagatgca gaatttattg cacgcaatgg gcgatgtgca tccgcacgcg ctcaaggcga
      181 agcttgcgcg cgaactcagc cgcttgacct ttgaaagatg gagcgcggcc aagttcaaag
      241 tccaaggttc aaagttcaag gttggcaaaa ccattttttg caattcggga tttgaagcgg
      301 tcgaagccgc gttgaagacg gcaatgcttg cgacgagcaa gccgggagtg atcgcgtttg
      361 agggcgcgta tcacgggctg ggttacggca cattgaacgc cacgcatcgc gaacattttc
      421 gcggatcctt catgaaacag attggcgggt tcgcggaatt cgtcggcttc ccaacgacgc
      481 cgcggcaaat gaagcgcagc ttgtccgaaa tccgcgattt gctgcattac aaggaaatcg
      541 gcgcggtgct ggtcgagccg gtgcaggcgc gcggagggat caatgtgcca ccgcaaacat
      601 ttctgccggc gttgcggaaa ttgtgcgatg aatacgcggc tgtgctggtg ctcgacgaaa
      661 tttacaccgg gttcggccgc accggaaaat ggtttgcctg cgagcacagc aaaacgatgc
      721 cggacatcat ttgtcttggc aaagcgttga cgggcggatt tccgttgtcg gcgtgcgtgg
      781 ggcgcgcgga tttgatggat gccgcctggc cggtttccgg cggcgaagcg atccatacga
      841 gcactttcct cgggcatccg gtcgggtgcg cgatggccct ggcgcagatc gaagagattc
      901 gcgcgaaaaa gctcgttcaa cgcagcgctc aacttggcca atatcttttg cgcgaattga
      961 aaaaaaatat ttcgcatccg caatttgata tttccattcg cggccttggc ctgatggcgg
     1021 gtgtggaact ttgtctgcat aaccgcgtcc cggcgaccat tgtttcgctt cgcatcatca
     1081 aggaaatgtt gcatcgcggt tttatcctgt tgcccgaagg ttcgcacgcg aatgtcatca
     1141 gcttcacccc gccgctcacc atcacccgcg cacagctcac cgcagccgtc aatgctctgg
     1201 gagccgcgct gtcatcacca gaatgaatat gcgctggaaa attcaacgga gtgttggatt
     1261 gttggagtct cggactttca agttgttcct gcccatccca tcactccacc actccatttc
     1321 cctcacccca tgaaattaaa tgaaatgaag caggtcctgg aatcggggcg gattcaactc
     1381 acgaaatctc tcgggcaaaa ttttttgcac gatggcaatc aacttcggtg catcgtggac
     1441 gcggcagaat taaaaaaaac cgatcgcgtg ctcgaagtcg gaccaggctt gggtcctttg
     1501 acggaattgc tgatcgagaa agcaggtgaa gtgctggcaa tcgaaaaaga ccggcgcttg
     1561 tttgagtttc ttcagcaacg atttgttg
//
LOCUS       DASZMM010000410         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2087298, whole genome shotgun sequence.
ACCESSION   DASZMM010000410 DASZMM010000000
VERSION     DASZMM010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087298"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1203
                     /locus_tag="VG754_09850"
     CDS             388..1203
                     /locus_tag="VG754_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF19563.1"
                     /translation="MNIKGKAAVITGAAGGIGRAVAIELAKRGVGHIALVDKTEAVHT
                     LAIEVNNQTGASTATGYTGDVTDDTFRGWVYEDMHEKHGIVQICVPAAGITRDGLSVK
                     YDKQSDRVVIYPIKTYRLVTEINLIAPVYWALEMVAGIADHRRKHGLKRWEPSEGMMG
                     SVIFIGSVASVGNKGQISYSATKAGLEGAASTLTAEAIYHGVRCGVIHPGFTDTPMVR
                     ALGEDFIQQNIIPQTQLRRLIMPEEIADAICFMISNAAISGSLWADAGWRPAA"
     gene            1548..2294
                     /gene="fabG"
                     /locus_tag="VG754_09855"
     CDS             1548..2294
                     /gene="fabG"
                     /locus_tag="VG754_09855"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWF19564.1"
                     /translation="MKELDGKVAIVTGGTRGIGQAIVLALTEMGAKVAFNYQNSREKA
                     DELCRVIESRGGCCRAFVADVTSEEEVKRMVHEINDSMGPISVLVNNAGITRDRSFLK
                     MNRAMWDEVLRVNLDGPFIVTHAVLPCMINLGWGRIINISSFVGLTGNFGQTNYAATK
                     GGLNSFTCALAREVARKGITVNSVAPGFISTDMTKDVPAPTLEQVKTLTPMGRLGNPE
                     EVANAVAFLASPRSSYITGHVMSVNGGLYM"
     gene            2438..2983
                     /locus_tag="VG754_09860"
     CDS             2438..2983
                     /locus_tag="VG754_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19565.1"
                     /translation="MERDFPILLAEDDNTVAVAKRAFRQAGLQNPLRVVRDGEQVIAY
                     LKGDGRYSDRERYPLPKLVLMGFNIARRTGLEVLEWIRTQSALKELPIVMLSASSLCP
                     ETDLQKARKLGVTAHLTRTPDFKELQHVYKIAVDHWNLLEMRPNRTAGKLAMRNWPRG
                     QKFCMPPMPRGHVDAMAQNRA"
     gene            complement(3191..3643)
                     /gene="dtd"
                     /locus_tag="VG754_09865"
     CDS             complement(3191..3643)
                     /gene="dtd"
                     /locus_tag="VG754_09865"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWF19566.1"
                     /translation="MRSVIQRVSSATVVIGGNVKSAIGRGLLVLLAVEDADTAEDIEW
                     LSAKIARLRIFDDTEGVMNLSAQEAQGEILVVSQFTLFASTKKGNRPSYIRSARPEVA
                     VPLYEQFIARLATDLGRPIQTGEFGADMQVTLTNDGPVTIIIDSKNRE"
     gene            complement(3858..4025)
                     /locus_tag="VG754_09870"
     CDS             complement(3858..4025)
                     /locus_tag="VG754_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19567.1"
                     /translation="MQVGDVVVSFAGQPVTGIDSLHKLLTDSRIGTQSPMTAIRHTEK
                     MELEVVPAESK"
BASE COUNT          934 a         1057 c         1115 g          928 t
ORIGIN      
        1 cagcgttaga tgctggctgc gtgtgacatc ataatagcgg cggttgggat atttccggat
       61 gtccaaaggt ttggaatgtg gtgcattggc ttcagccata agcgtaaaaa aaaccctgtt
      121 gtgcgatggc aataatttat cttcaatttg cgaggaaagt caacccggta atattttggc
      181 catgcgcggc acccgttctc aagggatttg taatcaatat catgcagaca tgtcagtttc
      241 cgcaacacgt ccgtattctg gattttttat ttatggtgca aatgcacaaa atccattgac
      301 tacacgtcct cgccggctta tggttttgca atagccaaca actcagccag gtcagcccaa
      361 aaccaagacc atcaaccaat acctaatatg aacatcaaag gaaaagcggc agtgatcacg
      421 ggagctgcag ggggcattgg acgcgcagtg gccatcgaac tggccaaacg aggcgtggga
      481 catatcgcgc ttgtggacaa gaccgaggca gtgcacacgc tcgcgataga agtcaacaat
      541 cagacaggcg cttcgacggc gacgggttac acgggggacg tgacagatga tacattccga
      601 ggctgggtct atgaggatat gcatgaaaag cacgggattg tgcagatctg cgtgccggcg
      661 gccggcatca ctcgggacgg tctgtcggtg aaatatgaca agcagagcga ccgggtggtg
      721 atctatccga tcaaaactta tcgcctggtg actgaaatca atctcatcgc gccagtttat
      781 tgggcattgg aaatggtggc gggcattgcc gaccaccgcc gcaaacatgg attgaagcgt
      841 tgggaaccga gcgaggggat gatgggcagc gtgattttta tcggctcggt cgcttcggtt
      901 ggaaacaaag ggcagatcag ctattcggcg acgaaagccg gcctcgaagg cgcggcgtcc
      961 acgttgactg cggaggcaat ttatcatggt gtgcgatgcg gagtgatcca tccaggattc
     1021 accgacacac cgatggtgcg cgcgttgggc gaggatttca tccagcaaaa catcattccg
     1081 caaactcaat tgcggcggct catcatgccg gaggaaatcg ccgatgcgat ttgtttcatg
     1141 atttccaacg cggccatcag cggctcgctt tgggcggatg caggctggcg gcctgcggct
     1201 tgaagcgggt tttaaagaaa tttttttctt acacggctgg tgtgttcttc tccccatttg
     1261 gggggagaag aacacgatga gacacaacga gatcgcctaa cttcaattcg gattgcgatg
     1321 gattttgcat cccccgcacg ggggagacga atccgttgtc acaaggattt taccctattt
     1381 tgtttgcaat cgttctccat ttttgttata gagcttcttg gacttagtaa ccatccaagc
     1441 aagcacgggg cggttcgggc tgacttcatt gtttttccgg ccgcaacgcc tgaacatgac
     1501 cggcagctac gcggtcatga ttaatcaacg agataaggag aacgaaaatg aaagaactgg
     1561 atggaaaggt ggctattgtc actggaggaa cacgcgggat cggccaggca attgtgctgg
     1621 cactcaccga aatgggcgca aaagtcgcct tcaactacca aaattcacgg gaaaaagcgg
     1681 acgagctttg ccgcgtgatt gagagcaggg gcggatgctg ccgtgcattt gttgcggacg
     1741 tcacctcgga ggaagaagtc aaacgcatgg tccatgaaat caacgactcc atggggccga
     1801 tcagtgttct cgtcaacaac gcgggcatca cgcgggaccg ttcgttcctg aagatgaacc
     1861 gggcgatgtg ggacgaagtc ttgcgggtga acctggacgg gccgttcatc gtcacgcacg
     1921 cggtgcttcc ctgcatgatc aatctcggct ggggccgcat catcaacatc tcgtcctttg
     1981 ttggattgac ggggaatttt ggccagacga attatgccgc caccaaaggc ggactcaatt
     2041 cgttcacctg cgcgctggcg cgggaagtcg cccgcaaagg catcaccgtg aattccgtcg
     2101 cgccgggctt catcagcacc gacatgacca aggacgttcc ggcgcccacg ctggagcagg
     2161 tcaagacctt gacgccgatg ggacgactcg gaaatcccga agaggtcgcg aatgccgtgg
     2221 ccttcctggc cagtccgcgt tcgagttaca tcaccggcca tgtaatgtcc gtgaacggcg
     2281 gactctacat gtgacgatcg gagaataatt gttccgcaaa agacatgttg cacaacatgc
     2341 cgggttggtt tttttagctg cggctgaata cgttttttta ttcatccgtg ttgattcaat
     2401 tcaatccggc gcatccgggt tttttaccca taacaaaatg gaacgtgatt ttccgatctt
     2461 attggcagag gacgacaaca ctgttgccgt cgcgaagcgg gcctttcgcc aggccggcct
     2521 tcaaaatcct ctgcgggttg tcagggacgg cgagcaggtc attgcctatc tgaagggcga
     2581 tggccgatat tcggatcgcg aacggtatcc acttcccaag ctggtgctca tgggattcaa
     2641 catcgcaagg cgcaccggcc tggaagtttt ggaatggata cgcacacaat ccgcgctcaa
     2701 agagctgccc atcgtcatgc tgtctgcttc ttcgctttgt ccggagacgg acttgcaaaa
     2761 agcgcgcaag ttgggcgtca ccgcgcacct cacgcggacg cccgatttca aagaactgca
     2821 gcacgtttac aaaatcgctg tggaccattg gaacctgctc gaaatgcggc ccaaccgcac
     2881 tgcgggaaaa ctcgccatgc gcaactggcc gcgcggccag aagttttgca tgccgccgat
     2941 gcctcgcggc catgtcgacg caatggccca aaacagggct taatgtccat tcaaggaagc
     3001 catggttttc tccgtggctt ccttctcata attagtggag cgcggctgtg cgcacagcgc
     3061 cagccgcagc agaccactaa tatcttgggc gttccgtgct gctgcggctg gtcctacgga
     3121 cacagccgcg ctccgttttc gacttaggac atgactcgtg ggcgcatacc gcatatcgca
     3181 agctttctga tcattcgcga ttctttgaat cgatgatgat tgtcaccggg ccgtcgttgg
     3241 tgagcgtcac ctgcatgtcc gcgccgaatt cgccggtctg gattgggcgg cccaaatcgg
     3301 tagcgagccg cgcgatgaat tgttcgtaaa gcggaaccgc gacttccggc cgggcggaac
     3361 ggatgtagga agggcgattg ccttttttcg tgctggcgaa cagcgtgaac tggctcacga
     3421 ccagaatttc tccctgcgct tcctgtgccg aaagattcat cacgccttcg gtatcatcga
     3481 aaatccgcaa gcgcgcaatt tttgcgctca accattcgat gtcctccgcc gtgtccgcgt
     3541 cctcgaccgc gagcaagacc agcaacccgc gcccaatggc gcttttcaca ttgccgccga
     3601 tgaccacggt ggcgctggag actcgttgga tgaccgagcg cattcggtta attctcgcgg
     3661 taaatcttca gcaattcttt caacccggta tcgtgaaaat gattgccgcc aaagaaatcc
     3721 aaaaacccaa cttggatttt ttgacagaat ttacagaata tgcagaacca aagatagcgt
     3781 tgaaacacga tttgttagat tctgatcatt ctgtaaattc tgtcacaaag acttttgttt
     3841 gggtctttgg atatttttta tttgctctcc gccggcacaa cttccaactc catcttctcc
     3901 gtgtgccgga tggccgtcat cggcgattgc gttccgatgc gcgaatcggt caaaagtttg
     3961 tgcaacgaat caatgcccgt caccggttgc ccggcaaaac tgacgacgac atcgccgacc
     4021 tgcaatccgg cttt
//
LOCUS       DASZMM010000411         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1814611, whole genome shotgun sequence.
ACCESSION   DASZMM010000411 DASZMM010000000
VERSION     DASZMM010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814611"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VG754_09875"
     CDS             complement(<1..482)
                     /locus_tag="VG754_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWF19568.1"
                     /translation="MKIIRAEHLGMCFGVRDAIALAKNTRAPLTILGDLVHNETVLAD
                     LRARGIQIKQKPDEIATQDVMITAHGASERAVQEIKRRGLNVLEATCPLVRFAHEAVA
                     ELAAAGFHPVIIGKRDHVEVRGLTGDLEAFDVVLDEADVMKLSERARFGIAAQTTQPI
                     "
     gene            complement(653..976)
                     /locus_tag="VG754_09880"
     CDS             complement(653..976)
                     /locus_tag="VG754_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWF19569.1"
                     /translation="MNPEPFLNLDRIIHEKGRLGIMSLLAATPELSFTELRDTLGMTD
                     GNVTTHIRTLQEAGYVSVAKSYENNRPLTTCTLTVAGRKAFTTYINLLEKIVQQTKSK
                     DTSEK"
     gene            complement(973..1626)
                     /locus_tag="VG754_09885"
     CDS             complement(973..1626)
                     /locus_tag="VG754_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19570.1"
                     /translation="METNWAAEHLQTIRTLMERSALYRRALAPISTFVGVVGIAGAIL
                     GWGLDIAVASEFTVLWMLVGIIAVSGALVLVRKQALKQGEAFWSPPMRRIVQAMVPAL
                     LIGFILGLPILIAEDAKLRAGTPQSPTALILTFIWTCLYGLALNAAGFFMPRGIRLFS
                     WLFIIGGIGLIFMKDFGPWDLESNPTKSANVLMGLVFGASHLAYGIYLYFTERKNEA"
     gene            complement(1798..2838)
                     /gene="lpxD"
                     /locus_tag="VG754_09890"
     CDS             complement(1798..2838)
                     /gene="lpxD"
                     /locus_tag="VG754_09890"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWF19571.1"
                     /translation="MPFTAAEIATQLQGQVRGDGAVVLSSFASADRARAGDLTFAENE
                     TFFHRAETSAASAILVDGDFTSEKKTLIKVANARIAFARVLAIFFPEPSFAAGIHPTA
                     IVAKSAQVDATAHVGPYCVVGEQARIGARAILQGANHVGAQSQLGDDSNLFPNVIIYP
                     RTQIGQRVRIHSGSVIGSDGFGYVFDQGEHRKVPQVGNVVIGDDVEIGANVTVDRGAL
                     GPTTIGKGTKIDNLVQIAHNVSIGEHSLIIAQVGIAGSSKLGNYVILGGQVGIAGHLK
                     IGNQVTIAAKAGVMRDVKDGEKLLGAPAIPDRQAKRQLIAVQQLPDLIKRVRELEKKL
                     GMGGSESGQAES"
     gene            2998..>3923
                     /locus_tag="VG754_09895"
     CDS             2998..>3923
                     /locus_tag="VG754_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HWF19572.1"
                     /translation="MQNNLSTINTATMPDDNSDGAAWVRIKDYDAKNGWGDAAQNAAR
                     SRWCCEQLEEGQILFFDSIPYDFPEADRQFLLEQRQSDSRIHKNISYRPNQDVLRGSV
                     ADSKEDIERLHGIMRHFSEEIVGLLTRVLAPYAPHWKLDYASYRPEEEQNRDLSLHKR
                     NDLLHVDAFPTRPTRGGRILRCFTNINPTRTRNWLTTDRFPALAEKFARNAGLEEIAR
                     SGDDNGSIVNMLKRAVGVKTVDRSAYDKFMLRFHDYLKENEDFQKNCRKIRINFPPMA
                     TWMCYTDSVPHAVLSGQYALEQTFIIPVRALVT"
BASE COUNT          898 a         1152 c         1014 g          859 t
ORIGIN      
        1 tcaatcggct gcgtcgtttg tgccgcgatg ccaaaacgcg cgcgttccga cagcttcatc
       61 acgtcggctt catcgagcac cacgtcaaaa gcctccaaat cacccgtcaa accgcgcact
      121 tccacgtggt cgcgcttgcc aatgatgacc ggatgaaacc ccgccgccgc caattcggca
      181 acggcttcat gcgcgaaacg aaccaacgga caagtggctt cgagcacgtt caacccgcgc
      241 cgtttgattt cctgcacggc gcgttccgaa gctccatgcg cggtgatcat cacgtcttgc
      301 gtggcgattt cgtccggctt ttgcttgatc tggatgcccc gggcgcgcag atcggcaagg
      361 acggtttcat tatggaccag gtcgccaagg atggtcagcg gcgcgcgggt gttttttgcg
      421 agcgcgatgg cgtcccgaac gccgaaacac atgcccagat gctctgcccg gatgattttc
      481 atgttaaaat gactttgtga cacaaagtgt gagactccga gggcttcgtt ttgttcaaaa
      541 ttttcatggg atgacttgga attttaacgg ttacaaagtt gcatcgttac aaggttacgt
      601 ggtgcaggcg ctcttgtaac catgcaacct tgtaatgatg caaccatttc cttcatttct
      661 ccgaggtgtc tttggacttg gtctgctgga cgattttttc cagcaggttg atgtaggtgg
      721 tgaaggcttt tcgtcccgcg acagtgagcg tgcaagtcgt gagcggacga ttgttttcgt
      781 aggatttcgc cacggaaaca tagcccgctt cctgcaacgt gcggatgtgc gtggtcacgt
      841 tgccgtccgt catgccgagc gtgtcgcgca attcggtgaa cgaaagctcc ggcgtggcgg
      901 ccagcaacga catgatgccg agccgcccct tctcgtgtat gatgcgatcc aggttgagaa
      961 atggttccgg attcacgctt cattcttccg ttcggtaaaa tacaaataaa tgccgtaagc
     1021 cagatgcgac gcgccgaaga caaggcccat gagaacattt gcagacttgg ttggattaga
     1081 ctctagatcc caagggccga agtctttcat gaatatcaac ccaattccgc caatgataaa
     1141 cagccagcta aacagtctta ttcctctcgg cataaaaaag ccggcagcat tcagcgccag
     1201 gccataaaga caagtccaaa taaacgttag aatcaatgca gtcggtgatt gcggggttcc
     1261 cgcccttaat tttgcatctt cagcaatcaa aatgggcaat ccaagaataa atccgattag
     1321 cagtgcaggg accatcgcct gaacgatgcg acgcatcgga ggcgaccaaa aagcctcccc
     1381 ttgtttcaga gcctgttttc gcaccaacac caaggctccg gaaaccgcga taattccaac
     1441 caacatccag aggacagtaa attcgctggc cacggcaata tctaatcccc agcccaatat
     1501 cgctccggca atccccacca cgcccacaaa cgtcgagatt ggcgcaagcg cgcggcggta
     1561 aagcgcggag cgctccatca atgtgcgaat ggtttgaaga tgttccgcag cccaatttgt
     1621 ttccatgcat acatactttg tatcacaaag tatgaatgtc aagtgaaaat ttgaaacccc
     1681 tttggaggct tttgtcaggg tttccgggcg gaagccttca ttatatctga aatctcaaat
     1741 ttcaaatctg aaatccaaat gaaaacccgt cacctcatct ctcacaggaa aattgactta
     1801 ggattccgcc tgcccgcttt cacttccacc catgcccagc ttcttttcga gttcacgaac
     1861 gcgctttatc aaatcgggta attgctggac ggcgatgagt tgccgcttgg cttggcggtc
     1921 gggaatcgcg ggcgcgccca aaagtttttc gccgtccttg acgtcccgca tgacgccggc
     1981 tttcgccgcg atggtcacct ggttgccaat tttcaaatgg cccgcgatgc ccacctgccc
     2041 gccaagaatc acataattgc cgagcttgga gcttccggcg atgcccacct gcgcgatgat
     2101 caaactgtgc tcgccgatcg aaacattgtg cgcgatttgg acaagattat cgatcttcgt
     2161 ccccttgccg atggtcgtcg gccccaacgc gccgcggtcc accgtcacgt tcgcgccgat
     2221 ttccacgtcg tcgccgatga ccacattgcc gacctgcggc accttgcgat gttcgccttg
     2281 atcaaacacg tatccgaagc cgtccgaccc gatgaccgac ccggaatgga tgcgcacgcg
     2341 ctggccgatt tgcgtgcgcg gataaatgat gacgttggga aaaagattgc tgtcatcgcc
     2401 caactgcgat tgcgcgccga cgtggttcgc tccctgcaaa atcgcgcgcg ccccgatgcg
     2461 cgcctgttcg ccaacgacgc aatacggccc gacgtgcgcc gtggcatcaa cttgcgccga
     2521 tttcgcgacg attgctgttg gatgaatgcc tgccgcaaac gaaggttcag gaaaaaaaat
     2581 cgccagcacc cgcgcgaacg cgatgcgggc attcgcgacc ttgatcaacg ttttcttttc
     2641 cgaggtaaaa tcgccatcca cgagaattgc cgaagccgcg cttgtttcgg cgcgatgaaa
     2701 aaaagtttcg ttctcggcga aagtgaggtc gccggcgcgc gcgcggtccg ccgaggcgaa
     2761 gctggaaagc acgactgcgc cgtccccgcg gacctggccc tggagttgcg tggcgatttc
     2821 agcggcggta aagggcataa gaaattgggc gtttcaacgg attgactata aaagtcgcgc
     2881 aaggcttgtt caacaccatt cggctgccga gtgtcagctt gactttttgg ccgttgagcc
     2941 acaatttgct gacactgagg gctgcgagaa atttgccccg aaatcatttt gacaaccatg
     3001 caaaacaacc tttcgaccat caacactgca accatgccgg acgacaattc agacggcgcc
     3061 gcctgggtgc gcataaagga ttacgacgcc aaaaacggct ggggcgacgc cgcccaaaac
     3121 gccgcgcgct cgcgctggtg ttgcgaacaa ttggaagaag gccagatact tttcttcgac
     3181 agcattccct acgattttcc cgaagccgac cgccaatttc ttttggaaca acgccaaagc
     3241 gattcgcgca tccacaaaaa tatttcctac cgcccgaacc aggacgtttt gcgcggcagc
     3301 gtggctgatt ccaaggaaga catcgagcgg ctgcacggaa tcatgcgcca tttttccgag
     3361 gaaatcgtgg ggttgctcac gcgtgtgctc gcgccttacg cgccgcattg gaagctcgat
     3421 tacgccagct accgtcccga ggaagaacag aatcgcgacc tttcgctcca caaacgcaac
     3481 gaccttctcc acgtggatgc ttttcccacg cggccaaccc gcggcggacg catcctgcgc
     3541 tgtttcacga acatcaatcc cacgcgcacg cgcaattggc tcaccaccga ccgttttccc
     3601 gcgctggccg aaaaattcgc gcgcaacgcc ggcctcgagg agattgcccg cagcggcgac
     3661 gacaacggtt caattgtcaa catgttgaag cgcgcagtcg gtgtcaagac cgtggaccgc
     3721 tccgcgtatg acaagttcat gctccgcttc cacgattatt tgaaggagaa cgaggatttc
     3781 caaaaaaatt gccgcaagat ccgcatcaac ttcccgccca tggcgacctg gatgtgctac
     3841 accgattccg tgccgcacgc cgtgttgtcc ggccaatacg cgttggaaca aaccttcatc
     3901 atccccgtgc gcgcgctggt gac
//
LOCUS       DASZMM010000412         7191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4571821, whole genome shotgun sequence.
ACCESSION   DASZMM010000412 DASZMM010000000
VERSION     DASZMM010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4571821"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VG754_09900"
     CDS             complement(<1..1277)
                     /locus_tag="VG754_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF19573.1"
                     /translation="MSRLPFELLLALRYLRPKRTFVSVITLISVLGVTLGVAVLIIVI
                     SVMSGFDRELRDKVFGFNSHLTIIETNPATGRQIEKMSDAASVMQTVNSNRLVKGSSP
                     FVMGQVVVQTEPEAGESQIQAPYLRGIDPRAQSTLGLVTSNIVSGTNDLSGRGLLLGT
                     DLADRLYVSVGDHVVVATPKDIQKMWSARTNGNQDITIPPDYEVRGIFNSGFYEYNST
                     IAITSLENAQDMFNLDDNVQAVLVMLHDPFDATKASAQISAALGKNYYVRSWENDSSG
                     MTAVLVEKNVMLYILFFIVIVAAFGITCTLVTFVVLKTREIGVMKALGASSPQISWIF
                     LCQSMGVSIMGVLIGTGLGLLAVAYRNPFLHLMRNLTGIELFPANIYNFSELPAQIVP
                     GDILIICGGSFLICLLAAAFPVWNASRLKPVEA"
     gene            1486..2595
                     /locus_tag="VG754_09905"
     CDS             1486..2595
                     /locus_tag="VG754_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19574.1"
                     /translation="MRARDHFKISGFLLILSMVVGLRAMGGKASAADNTNAVSDAEGK
                     VAEAALITNQESIRSNLQIQEQLHDTLLAIEKNRQEAEAAAARNAKMLEERLNLMEKT
                     LAEQRSNYLSDIERADRMMNIEHTDRMMMMVSGGFAAIGFVVLLAAAFLQWAGANRLA
                     TLAAALPDRRPTPVLGFDETKLLASSQAMEQSNARFLSIIERLEQRIQAMEITPKSPQ
                     HLPEASSTNGAEKSVEILAVAESAATTTEPASSADTDKAEAARLLLAKGQTLLKLDKP
                     QEALDCFDEVLALDPEHADALVKKGAALERLQRLDEAIECYDLAIARDNSLTMAYLYK
                     GGVFNRMERYSEALACYELALKSQQKGHAANVIID"
     gene            complement(2596..3090)
                     /locus_tag="VG754_09910"
     CDS             complement(2596..3090)
                     /locus_tag="VG754_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19575.1"
                     /translation="MLRLFLILSVVLLLNVSAQAVQPPPTSINHTPQGSVLKVLPQFL
                     DQKGQSALTASLYDRDAYQAFLRKNPRKRSTLRFAVQWKAKVPESDPLSLRVEIIGAA
                     KTDMPRQTSIETAVRQHHWFSHWTYLTMPKEQYKDVGDVTAWRVTLWDGDKMLSEQKS
                     FLWQ"
     gene            complement(3174..6140)
                     /locus_tag="VG754_09915"
     CDS             complement(3174..6140)
                     /locus_tag="VG754_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HWF19576.1"
                     /translation="MLLLVLVVLLAWVNTIGIPGFLKRPLIAELHAHGLDLQFSRMRW
                     RFYRGLVADDVRLGALQQETNTPQLSVRELALKINHHDLIRFKLKIDSVTLRGGRLFW
                     PLAETNQPASSLSITNIQTQLRFLPGDQWTLDNFSAGLAGAKLQLSGTLTNASAVRDW
                     KTLHAHGKTQPGLLNQHLREIARVIDQIQFTKPPQLDVFVNGDARDPLSFSGVLTLGA
                     QGAMTPWGTVSNADFLLRLVPPSGPNKFPRMKCQLHTDELNFELHPDAFRTLAGDTRH
                     FHLDVEATVDEASTNQVRARMELTANRLNTPWAQATNVHFAARWTHSLTNPIPIAGTG
                     DLRLTDAHTKWGSAGQLELSANLSQSPAGQPMQANADWAWWTALEPYALDWNCRLENI
                     HAEDFKSAEVVFGGNWRAPELTVTNLHTEMYRGRFDANAGLNVATREARFLCTSDFDA
                     MKIQPFLTPGARRWLDDQQFEWEKPPLLQGSGSVILPAWTNTHPDWRGEVLPTFSLSG
                     EFKVGAASYRKIPMTSAQSHIYYTNMIWNLPDLVANRPEGKVELSHVSDDRTKEYYFR
                     FRSTVDVKLARHLLATNAQRGLDAFTFSKPPMIDGEVWGRWRSHERIGFKGQIEVTNF
                     VFRGASANYFHTFLEYTNVFLQLRDARLERTNQYLAASKMIIDFAGKRAFLTNGFSTM
                     DPMIVLRSIGPHVARDMEPYHFLKPPVIKANGIIPLSDDTSVADLHFVVDGGPFEWLN
                     FYLPQISGTVDWKGELVTLEQMKATFYEGKLTGSAGFDFKRKVGTDFHFDTIVTGANL
                     HLLMADLTKGTNHLEGTLNGHLNIAEANSNDPHSWFGKGEADLHDGLIWDIPIFGILT
                     PVLDAISPGLGKTRANEAAGDFIITNGVIRSDNLVIRSPTLTLFYKGTVDFNRKVDAV
                     VDAEVGRETPIVGPVIGALSAPFGKLFETRVTGSLENPKREAVIPFRWLSPFHWLQML
                     KELAPPGGQENNPQPK"
     gene            complement(6219..>7191)
                     /locus_tag="VG754_09920"
     CDS             complement(6219..>7191)
                     /locus_tag="VG754_09920"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWF19577.1"
                     /translation="ARFNGQNSIVLVPRNQVAPWTAGFQEFRDRHLVRLADGPPDVIE
                     VTGAGKDENFTVQHQADDSWRVIKPLDLPADPKIMRAFLTNLTGIEVLRSDNRVAVDD
                     TVPPDSPLYGLATPARRYILKRTNNSKNEIMAELDFGSAKDGHIFARRADLPQESSVY
                     AVKLEDFQKLPATALQLRDRRIWDFSETNVSSITICQNGKNEQLVHKGPNKWTIGTGS
                     GMINELEVEVAAQELGFLAADNWIQRGDTDRERYGFTNKLLQISVDLKEGDRPKTLTV
                     YLGGVAPNGLRYGDTQLDDGLNWIFEFQPKFLDELNAYLNIHENPGP"
BASE COUNT         1645 a         1897 c         2066 g         1583 t
ORIGIN      
        1 aaagcctcca ccggcttgag ccggctggcg ttccaaactg gaaatgccgc cgcgagcaag
       61 cagatcaaaa acgaaccgcc gcagatgatc aaaatgtcgc ccggcacaat ctgcgcgggc
      121 agttcgctga agttgtaaat attcgcgggg aacaattcga tgccggtcaa gttgcgcatc
      181 aagtgcagga atggattgcg ataggcgacc gcaagcaaac cgagtccggt cccgatgagc
      241 acgcccatga tgctgacgcc catgctttgg cacaaaaaaa tccaactgat ttgcgggctg
      301 cttgcgccca gcgctttcat cacgccgatt tcccgcgtct tcaggaccac gaacgtcacc
      361 agtgtgcagg tgatgccaaa tgcagcgacg atgacgatga agaacaatat gtaaagcatc
      421 acgtttttct cgacgaggac cgccgtcatg ccggaactgt cgttctccca tgagcgaacg
      481 taataatttt ttcccaacgc ggcgctgatt tgagcggaag ctttcgtggc gtcaaacggg
      541 tcgtgcaaca tcaccaaaac ggcttgaaca ttgtcatcga gattgaacat gtcctgcgca
      601 ttttcgagcg acgtgatggc gatggtggaa ttgtattcgt agaagccgga gttgaagatg
      661 ccgcgcactt cgtaatccgg cggaatggtg atgtcctggt ttccattcgt gcgggcgctc
      721 cacattttct ggatgtcctt gggcgtggca acgacgacgt ggtcaccaac actcacataa
      781 aggcggtcgg cgaggtcggt gccgagaagc aagccgcgtc cgctcaggtc attcgtgccg
      841 gaaacgatgt tgcttgtgac tagcccgaga gtgctttggg cacgtggatc aattccacga
      901 aggtagggcg cctggatttg cgattcaccg gcttcaggtt cggtttgcac caccacctgc
      961 cccatgacga acggggatga acccttgacg agacggttgg aattgaccgt ttgcatgacc
     1021 gatgcggcat cggacatttt ttcaatctga cgaccggtgg cggggtttgt ctcgatgatg
     1081 gtcagatgcg aattgaatcc gaaaactttg tcgcgcagtt cccggtcgaa tccgctcatg
     1141 acgctgatga caatgatgag caccgcgacg cccagcgtga cgcccaggac tgaaatcagg
     1201 gtgatgaccg agacaaacgt ccgtttgggc cgcaggtacc gcaaagccaa aagcaattca
     1261 aacggcaaac gcgacatgca gggaggctaa tattcattga gcagggtgtc aacgagggta
     1321 aaactccaag caccaatcca agctccaaag aaacaacaag ctcgcaaact tcaaattgtt
     1381 gattcgatta ttggagcttc tctggaggtt ggagctgggt gcttggaatt ttgattggtt
     1441 tggtcttgca ttttatggcc gattttttac cgttcaaggc cagctatgcg agcacgcgac
     1501 catttcaaaa tttccggttt tcttttgatt ttatccatgg tggttggtct tcgcgcgatg
     1561 ggaggaaaag ccagcgccgc cgataatacg aatgcggttt ccgacgcgga aggcaaggtg
     1621 gccgaagcgg cgctgatcac caaccaggag tcgattcgtt ccaatcttca aatccaggag
     1681 caactgcacg atacgctttt ggcgattgaa aaaaaccgtc aggaagccga ggccgcggcg
     1741 gcgcgcaatg ccaaaatgct ggaggagcgc ttgaacttga tggagaaaac gctggccgag
     1801 caacggagca attatttgag cgacatcgaa cgtgcagatc gaatgatgaa catcgaacac
     1861 acggaccgga tgatgatgat ggtctccggc ggttttgcgg cgatcggttt cgtggtgttg
     1921 cttgcggccg catttttgca atgggccgga gcaaatcgcc ttgccactct cgccgccgca
     1981 ttgccggaca gacggcccac gccggtgttg ggattcgacg aaacaaaatt gctggcgtcg
     2041 tcgcaggcga tggaacaatc caatgcccgt tttttgagca tcatcgaacg gcttgaacaa
     2101 cgcatccagg cgatggagat tacgccgaag tcaccgcagc atttgcccga ggcaagttcc
     2161 accaatggcg cggagaaatc tgtggaaatc ctggccgtgg cagaatccgc cgcgaccacg
     2221 acggaacctg cctcatccgc cgacacggac aaagcggaag ccgccagatt gttgctggcc
     2281 aagggccaga ctcttttgaa attggacaag cctcaggaag cgctggattg ttttgacgaa
     2341 gttttggcct tggaccccga acacgccgat gcgttggtca aaaaaggcgc ggcgttggag
     2401 cgcctgcaac gtttggatga agccattgaa tgttatgatc tcgccatcgc ccgggacaat
     2461 tcattgacca tggcttatct ctacaaaggg ggagtgttca atcgcatgga gcgctacagc
     2521 gaagcgctgg cctgttacga attggcgttg aaaagccagc aaaaaggcca cgccgccaat
     2581 gtgatcattg attgatcact gccacaaaaa cgatttctgt tcggacagca ttttatcgcc
     2641 gtcccataaa gtcacacgcc atgcagtgac atctccaaca tccttgtatt gctccttggg
     2701 catcgtcaga taagtccaat ggctgaacca atgatgctgc ctcaccgccg tttcgattga
     2761 cgtttgcctg ggcatgtccg tttttgcggc gccgatgatt tccacacgca ggctgagcgg
     2821 atcgctttca gggaccttcg ccttccattg caccgcgaag cgcaaggtcg agcgtttgcg
     2881 gggatttttc cggagaaaag cctggtacgc atcgcggtca tataggctgg ccgtgagggc
     2941 tgactggccc ttttgatcga gaaattgagg cagcaccttc aggacgctgc cttgaggcgt
     3001 gtggttgatc gaggttggag gaggttgcac tgcctgagcg gaaacattca gcagcaagac
     3061 gacggataaa atgagaaaca gccgaagcat caccggaaag gtatcgcgcg gccgggcaaa
     3121 ctcaatcccg aaaaaatgac attttctgcg actgtaaaat aaatcgaatg aaatcatttc
     3181 ggttggggat tattttcctg tccgccgggc ggggccaact ctttcaacat ttgaagccaa
     3241 tggaacgggc tcaaccaccg gaatggaatc acggcttcgc gcttgggatt ctcaaggctt
     3301 cccgtgactc tcgtttcaaa aagttttcca aaaggcgcgg aaagcgcgcc gatcaccggg
     3361 ccgacaatcg gggtttcgcg gccaacttcg gcatccacaa cggcgtccac ttttcggttg
     3421 aaatccaccg ttcccttgta aaacaaggtc agcgtgggcg agcggatcac cagattgtcg
     3481 gaacgaatga cgccgttggt gatgatgaaa tccccggcgg cctcgtttgc gcgtgtcttg
     3541 ccgaggccgg gcgaaatggc atcgagcacc ggggtgagaa tcccaaaaat cgggatgtcc
     3601 cagatcaatc cgtcgtgcaa gtccgcctca cctttgccaa accaactgtg cggatcgttt
     3661 gaattggctt cggcgatgtt caggtggccg ttgagcgtgc cttcaagatg atttgttccc
     3721 ttggtaaggt cggccatgag caggtggagg ttcgcgccgg tcacgatggt gtcgaaatga
     3781 aaatcggtgc cgactttgcg cttgaaatcg aatcccgccg agccggtcaa tttgccttca
     3841 taaaaagtgg ccttcatctg ctcgagcgtc acaagctctc ccttccaatc gacggtgccg
     3901 gatatttggg gcagataaaa attgagccat tcgaacgggc cgccatccac cacgaaatgc
     3961 aggtccgcga cgctcgtatc gtcggaaagc gggatgatgc cgttggcctt gatgaccggc
     4021 ggtttgagaa aatggtatgg ctccatgtcg cgcgcgacat gcggaccgat ggagcgcaac
     4081 acgatcatcg ggtccatggt gctgaagccg ttggtcagga aggcgcgttt gccggcaaaa
     4141 tcaatgatca tttttgacgc ggcaagatat tgatttgtgc gctcaagccg cgcgtccctc
     4201 aattgaagga aaacattggt gtactcaaga aaagtatgaa aataattggc tgacgctccg
     4261 cgaaaaacga aattggtgac ctcgatctgc cccttgaatc caatgcgttc atgcgaacgc
     4321 caccggcccc acacttcgcc gtcaatcatc ggcggcttgc tgaaggtgaa cgcatcgagg
     4381 ccgcgttgcg cgttggtggc aagcagatgc cgcgcgagtt tcacgtccac cgtgctgcgg
     4441 aaacgaaaat aatattcctt cgtcctgtcg tccgaaacat gggacaattc cacttttccc
     4501 tcgggacgat tcgcgaccag gtccggaagg ttccagatca tgttcgtata ataaatgtgc
     4561 gactgcgctg aagtcattgg aattttgcga taagacgccg cgccgacttt gaactctcct
     4621 gacaaggaaa acgtcggcag cacttcaccc cgccaatcag gatgggtatt ggtccatgcg
     4681 ggcaaaatca ccgagccgct gccttgtaaa agcggcggtt tttcccactc gaattgctgg
     4741 tcatccagcc atctgcgcgc gccgggcgtc aggaacggct ggattttcat cgcgtcgaaa
     4801 tcggaagtgc agagaaaacg cgcctcgcgc gtggcgacgt tcaggccggc attggcatcg
     4861 aaccggccgc ggtacatttc cgtgtggaga ttggtgacgg tcagttccgg cgcgcgccag
     4921 ttgccgccga agaccacttc ggccgacttg aaatcttccg cgtgaatatt ttccaaacgg
     4981 caattccaat ccagcgcgta gggttccagc gctgtccacc aagcccaatc cgcattggcc
     5041 tgcatcggct ggccggcggg cgattggctc aagtttgcgc taagttccaa ttgaccggcg
     5101 ctgccccatt tggtgtgggc gtcggtcagg cgcaaatcac ccgttccggc gatgggaatc
     5161 ggattggtga gcgaatgggt ccaacgcgcg gcgaaatgaa cattggtggc ctgcgcccat
     5221 ggcgtgttga ggcggttcgc ggtcaattcc atccgggccc gcacttggtt tgttgacgcc
     5281 tcgtcaaccg tcgcttcgac atcgagatga aaatgccgcg tgtcgccagc caatgttcga
     5341 aaagcatccg gatgcaattc aaaattcaac tcgtccgtgt gcaattggca tttcatccgg
     5401 ggaaatttgt tggggccgct cggagggacg aggcggagaa gaaaatcggc gttgctgacc
     5461 gtgccccaag gggtcatcgc gccttgcgca cccagtgtca agacgccgct gaagctgagc
     5521 ggatcacgcg catcgccgtt gacgaacaca tcgagttgag gcggctttgt gaattggatt
     5581 tggtcaatga cacgcgcgat ttcgcgcagg tgttgattga gaaggccggg ctgggttttg
     5641 ccatgggcat gaagcgtctt ccaatcgcgc acggccgagg cattggtcag cgtgccggaa
     5701 agttgcaact tggctccggc aagaccggcg ctgaaattgt cgagcgtcca ttggtcgcca
     5761 ggcaggaagc ggagctgcgt ctggatgttg gtgatggaga gagatgacgc gggttggttg
     5821 gtttcggcca gcggccagaa gagacgtccg ccgcgcaaag tgacggaatc aatcttcaac
     5881 ttgaacctta tcaagtcgtg atggttgatt ttcagcgcca gttcccggac agagagttgc
     5941 ggagtgttgg tttcctgttg caacgcgccg agacggacat catccgcgac caagccgcgg
     6001 tagaagcgcc agcgcatgcg gctgaattgc aagtccaatc catgcgcgtg caattccgcg
     6061 atcaacgggc gtttgaggaa accgggaatg ccaatggtat tgacccatgc gagcaagact
     6121 acgaggacca aaaggagcat caaaataacg gtccggattc cccgaaaaca aatccgggaa
     6181 attcgccacc aacatttttg ccctttttcc ggcatggttc acggtcccgg attctcgtgg
     6241 atgttgaggt aagcgttaag ttcgtctaga aatttgggct gaaattcaaa gatccagtta
     6301 agcccatcgt ccaactgcgt atcgccgtag cgcaatccat tgggagcgac gccgccgaga
     6361 taaaccgtga gcgttttggg cctgtcgcct tccttcaggt caacggaaat ctggagcagc
     6421 ttattcgtga atccgtagcg ttcgcggtct gtgtcgcccc gttggatcca attatccgcc
     6481 gccaaaaaac caagctcctg cgcggcgact tccacttcca gttcgttgat cattcccgaa
     6541 cccgtgccga tggtccattt attcggcccc ttgtgaacca gttgttcgtt tttcccattc
     6601 tggcaaatgg tgatcgaact gacattggtc tcggaaaaat cccagatgcg tcgatcacgc
     6661 agttgcaaag cggtcgcggg cagtttctga aaatcctcca gcttgacagc ataaactgaa
     6721 ctttcctggg gcaaatcggc gcgccgggca aagatgtggc cgtccttcgc cgaaccgaaa
     6781 tccagttcgg ccatgatttc gttcttggaa ttattggtcc gtttgaggat gtaacgacgc
     6841 gccggagttg cgaggccgta cagcggagaa tccggcggca cggtatcgtc caccgcgacg
     6901 cgattatcac tgcgcaaaac ttcaatgcct gtcaggttgg tgaggaacgc gcgcatgatt
     6961 tttgggtcgg cgggcaagtc gagcggtttg atgacccgcc acgagtcgtc agcctggtgt
     7021 tgcacggtga aattttcatc cttcccggcg ccagtgacct cgatgacatc gggtggccca
     7081 tcggcaagcc gcacgagatg gcggtcgcga aattcctgaa aaccggctgt ccacggagca
     7141 acctggttgc ggggcaccag cacaatcgaa ttttgcccgt tgaaccttgc a
//
LOCUS       DASZMM010000413         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5396724, whole genome shotgun sequence.
ACCESSION   DASZMM010000413 DASZMM010000000
VERSION     DASZMM010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5396724"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3044)
                     /locus_tag="VG754_09925"
     CDS             complement(<1..3044)
                     /locus_tag="VG754_09925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWF19578.1"
                     /translation="MSHADFVHLHLHTEYSLLDGACRLDRLMDKAHELKFPALAVTDH
                     GVLYGAVEFYQKAREKGIKPIIGCEVYVAPGSRFEKKTTSGGRDVYNHLVLLARDETG
                     YKNLIKLATAAHLEGYYYKPRIDREILEANKEGLIALSGCLASQIPEAILKDQMPKAR
                     ETIDWFKQIFGAENFYLELQNHGIPEQAKVNRYLIPWAKEFGLKLVATNDVHYIEKGN
                     SHAHDCLICIGTQTLLADTKRMTYVAEQFYLRSAEEMKARFSEVPEAVVNTLEVAEKC
                     SLEIQFGKLHYPVFHPPEHFTREGYLRKSLAEGLHRRYGIHAHAEGEQFIVEKIDDAW
                     RLPTFKLADDATRDPQQAITGLNDAAVSEAVKAVLSRLELELKVIEKTGFISYFLIVG
                     DFVRYGREHGISCVARGSAAGSIVTYLLEISNVDPIRYGLLFERFLNPERVNPPDIDI
                     DFADDRRADVIEYVRKKYGNDSVAQIITFGTMGAKSVVRDVGRVMGLSYGECDRLAKL
                     IPLGLEMDLSTALKQSPELKTAYDTEEVTRELIDTAFVLEDLTRNASVHAAGVVIGDQ
                     PLVNLLPLKQDEDGTLVTQYAMNPVGELGLLKMDFLGLKTLTVIRNTCEMVKATKGID
                     VPIDHLPLDDAKTYELLNKAQTLGIFQLESGGMRDLCRKFQISSVEHITALIALYRPG
                     PMELIPEFIKRRHGEVRIEYEHPLLEPICRETYGIMVYQEQVMQAAQVLAGYTLGGAD
                     LLRRAMGKKKVEEMQKQRDTFVKGCAKTNGIPTAKANQIFDLLEKFAGYGFNKSHAAA
                     YAIVAYQTAYLKANYPVEFLSAMMTNDMSDTKKLSEFIAEARTMGIEVLAPDVNESQV
                     VFAPARGGKVIRFGLAAIKGVGEVAVESILEARKQSGEFKSLGDMCERVDIRTVNRKV
                     LEALIKSGACDAFKETRATLFAQIDRTLSRAAGIIQDRQRGQSSMFGMLEEKTPEKQE
                     PVLRLPEWPQHELLAAEKELLGFYVTGHPLTPFAPVLQKYALA"
     gene            3193..3741
                     /locus_tag="VG754_09930"
     CDS             3193..3741
                     /locus_tag="VG754_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWF19579.1"
                     /translation="MQKILDSPKNDGVLELIACRPKAGERETLETAELHLAEGVVGDN
                     WKMRGAADKTANPDMQVTVMNSRVIALLADDKAHWPLAGDQLYVDFDLSIENLPPGSK
                     VGIGSAILQISPQPHTGCKKFLARFGQDALEFVNSAIGKQLRLRGLNARIVQPGIIRV
                     GDKVRKLTSEKNTQMPNDEILK"
BASE COUNT          875 a         1107 c         1089 g          943 t
ORIGIN      
        1 gccagtgcgt atttttgcaa caccggcgcg aacggcgtca atggatggcc ggtgacatag
       61 aacccgagca attccttttc cgccgcgagc aattcgtgct ggggccattc gggcaaacgc
      121 aaaaccggtt cctgtttctc cggcgttttt tcttccagca tgccgaacat cgagctctgg
      181 ccgcgttgac ggtcctgaat gatgccggcg gcgcgtgaca acgtgcgatc aatctgcgcg
      241 aacaatgtcg cgcgtgtttc cttgaaggca tcgcacgcgc ccgatttgat caaggcttcg
      301 agcactttgc gattgacggt gcgaatatcc acccgttcac acatgtcgcc gagtgatttg
      361 aattcgccgc tttgtttgcg cgcctccaaa atgctttcga ccgcgacttc gccaacgccc
      421 ttgatcgcgg caagcccaaa acgaatgacc ttgccaccgc gcgccggggc gaaaacgacc
      481 tggctttcat tgacgtccgg cgcaagcacc tcaatgccca tggtccgcgc ctcggcgatg
      541 aattcactga gcttcttggt gtcgctcatg tcgttcgtca tcatcgcgct gagaaattcc
      601 accggataat tggccttcag atacgcggtc tgatacgcga cgatggcata agcggcggcg
      661 tgcgatttgt tgaagccgta tccggcaaat ttttcgagca agtcgaaaat ctggtttgct
      721 ttcgctgtgg gaatgccgtt cgttttcgcg caacctttca cgaaagtgtc gcgctgcttt
      781 tgcatctcct cgactttttt cttgcccatc gcgcgccgca acaaatccgc gccgccgagc
      841 gtgtaacccg ccaacacctg tgccgcctgc atcacctgtt cctgataaac catgatgccg
      901 taggtttcgc ggcaaatcgg ttcgagcaac gggtgttcat attcaatcct cacctcgcca
      961 tggcggcgct tgatgaattc cggaatcagt tccatgggcc ccggacgata aagcgcgatc
     1021 aaggccgtga tgtgttcgac cgaactgatc tggaatttgc ggcacaagtc gcgcatcccg
     1081 ccggattcca actggaagat gccgagcgtc tgcgccttgt tcaaaagctc gtaagtcttc
     1141 gcgtcatcga gcggcagatg gtcgatcggc acgtcaatgc cctttgtggc cttcaccatt
     1201 tcgcaggtgt tgcgaatgac cgtcagcgtc ttgagcccga gaaaatccat cttcaataga
     1261 ccaagttcgc cgacgggatt catcgcgtat tgcgtgacca gcgtgccgtc ttcgtcctgc
     1321 ttgagcggca gaagattcac aagcggctgg tcaccgatga caacgccggc ggcgtgaacg
     1381 gaagcatttc gtgtaaggtc ttcaagcacg aacgcggtat caatcaattc gcgcgtgact
     1441 tcctcggtgt cgtaagccgt cttgagttcg ggcgattgct tgagcgccgt gctcaagtcc
     1501 atctccaaac cgagcgggat caatttcgcc aggcggtcgc attcgccgta actcaagccc
     1561 atcacgcggc ccacgtcgcg caccaccgat ttcgcgccca tcgtgccaaa cgtgatgatt
     1621 tgcgcgacgg aatcgttgcc gtattttttg cggacatatt cgatgacgtc cgcgcggcgg
     1681 tcgtcggcga aatcaatgtc aatatcgggc ggattgacgc gttcgggatt gaggaagcgc
     1741 tcgaacagca gcccataacg gatcggatcc acgttcgaaa tctccagcag ataagtgaca
     1801 atcgagcctg cggccgaacc gcgcgccacg caggaaatgc cgtgctcgcg tccgtaacgg
     1861 acaaaatcgc ccacaattaa aaaatagctg atgaacccgg tcttctcgat gaccttgagt
     1921 tccagttcaa ggcgcgagag gacagccttc acagcttctg aaacagcagc atcgttcaag
     1981 ccggtgattg cctgttgcgg gtcgcgtgtc gcgtcatccg cgagcttgaa cgtcggcaaa
     2041 cgccacgcgt catcaatttt ttcgacgatg aactgttcgc cttcggcgtg ggcgtggatg
     2101 ccataacggc gatgcaagcc ttccgcgagc gatttccgca gataaccttc gcgcgtgaaa
     2161 tgttcgggcg gatgaaagac cgggtaatgc agtttgccga actggatttc gaggctgcat
     2221 ttctcggcca cttcgagagt gttgacgacc gcttcgggaa cctcgctgaa acgcgctttc
     2281 atctcctcgg cggaacggag ataaaactgc tcggccacat acgtcatccg tttcgtgtcc
     2341 gccaagagcg tctgggtgcc gatgcagatg aggcaatcgt gcgcgtgcga attgcctttt
     2401 tcgatgtaat gaacatcgtt cgtggcgaca agtttcaggc caaattcctt cgcccatgga
     2461 atgagatagc gattgacctt tgcctgttcc gggatgccat ggttttgcag ttcgagataa
     2521 aaattttccg cgccgaagat ttgtttgaac cagtcaatcg tctcgcgcgc cttgggcatc
     2581 tggtccttca aaatcgcctc cggaatctgg cttgcaagac agccggaaag cgcgatcagg
     2641 ccttccttgt tggcttcaag aatttctcga tcaatgcgcg gcttgtaata atagccctcc
     2701 agatgcgcgg cggtggcgag cttgatgaga tttttgtagc cggtttcgtc gcgcgcaagc
     2761 aggacaagat gattatagac atcgcgcccg ccgctggtgg ttttcttttc gaaccggctg
     2821 ccgggagcca cataaacttc gcagccgatg atgggcttga tgcctttttc gcgggccttc
     2881 tggtaaaact caactgcgcc ataaagcacg ccatggtcgg tgacggccag cgcgggaaac
     2941 ttcagttcgt gagctttgtc catgagccgg tcaagccggc aggcgccatc gagcagcgaa
     3001 tactcggtgt gcagatgtaa atgaacaaag tcagcatgcg acatgaaaag attttaccgc
     3061 cgcatgcgga ggttgcgcaa ggcggagtta tgaacattgc gaacatgact gctttaattt
     3121 ttgccagctt ggtgtaaaat cctcgcagtg attcaaccag atcatctccc aagagctgaa
     3181 ctcgacgccg gaatgcaaaa aattctcgac tcgccgaaga acgacggcgt gctggaattg
     3241 atcgcgtgcc gtccgaaagc cggcgagcgg gaaacgcttg aaaccgcgga actccatctc
     3301 gccgaggggg tggttggtga taattggaaa atgcgcggcg cggcggacaa gacggccaat
     3361 cccgatatgc aggtcacggt gatgaattcg cgcgtgatcg cgctgctcgc cgacgacaaa
     3421 gcgcactggc cgctcgcggg cgaccagctt tacgtggact ttgatttaag catcgaaaat
     3481 ctgccgccag gctcaaaggt gggtatcggc tcggccatcc ttcagatttc cccacaacct
     3541 cacaccggct gcaagaaatt cctcgctcgc tttggccagg acgccttgga gtttgtgaat
     3601 tcggccatcg gcaagcagct tcgtttgcgc ggattgaatg cgcgcattgt gcagcccggc
     3661 atcattcggg tgggcgacaa ggttagaaaa cttacaagcg agaaaaatac ccaaatgccc
     3721 aatgacgaaa tactcaaata aacccgaaat tccaagctgc gatttttgac agaatgaaca
     3781 gaatgttcag aatggaacac tttctgaaaa tcatattttc tttcttgata cgtttattct
     3841 ctcaaaataa tcccgcatcg tgcattccag cttgagattt ttccggtgtt ccgggtgttt
     3901 gggtactggt atttaattcg gatctacctc gaaaccttgc tctcgcaagc gctgtagttt
     3961 gtaaagcaac gcccttctgc tgattcccaa ggtcttcggg gtttcggtgc ggtt
//
LOCUS       DASZMM010000414         5398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_464981, whole genome shotgun sequence.
ACCESSION   DASZMM010000414 DASZMM010000000
VERSION     DASZMM010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464981"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="def"
                     /locus_tag="VG754_09935"
     CDS             <1..617
                     /gene="def"
                     /locus_tag="VG754_09935"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWF19580.1"
                     /translation="QFILYQMILEVVKYGDPVLRAKGARIETITPAIGRLIDDMFETM
                     YHARGIGLAAQQVGHALQLTVIDVREAVDRPSTLELDGKPAEPNDFMPLVLINPQVNA
                     VKTSAKAEGPEGCLSFPEIFADITRPESVDVVAMDRDGKRVEFRCGGLLARAVQHETD
                     HLNGILFIDRMDSETKSNLKPEIEDLQAATKAALQKKKKMPPVL"
     gene            complement(643..1017)
                     /locus_tag="VG754_09940"
     CDS             complement(643..1017)
                     /locus_tag="VG754_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19581.1"
                     /translation="MTLWFVALKPTKEVFETMYLASVMDQANIAMRIRASQEMALVSN
                     IESNFPQTVLAVHQAFRNNAGSTNALWMIKAYYQLNNIKVPEEIRGILDSLPEKPPTS
                     CQMRLRELERQSKTNAPAAPAK"
     gene            complement(1236..1805)
                     /locus_tag="VG754_09945"
     CDS             complement(1236..1805)
                     /locus_tag="VG754_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19582.1"
                     /translation="MQPFMKYLSFAFLCALLCGCGNSVPTTPQGRLTEAIKKLNQAST
                     DEERFYALNDAAKESFEVDKIDDAHKYATNLLALAKKFQGNWNYGNAIHDGNVVLGRI
                     AVKEGKIDEAKNFLLAAGKSPGSPQMDSFGPNMSLAQDLLLKGEKDTVLEYFNLCSKF
                     WSKNFSKLDQWSSDVKAGRMPDFGANLVY"
     gene            complement(1860..3341)
                     /gene="rpoN"
                     /locus_tag="VG754_09950"
     CDS             complement(1860..3341)
                     /gene="rpoN"
                     /locus_tag="VG754_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWF19583.1"
                     /translation="MTQSLVLSPQMQQSLALLQAPTLELKALVEQELQQNPVLEEAPT
                     PEAEQSEKDSKEDGDGPETDSAESSDPAEPPSDVTFDNTTEKNGEPADDFMAEFEKLA
                     QMDQDWRDHFAATNIPMRQSAEDEEKRQFMFDSIVAATSLQEILLEQVRDSRLNDEQR
                     PIAEMLIGNIDDYGYLKTSIEELAFSTNLPAEKIEAVLRVVQTFDPPGVGARDLQECL
                     LRQLERACDEKSVEYRIVKDYMDALGKRRIPEIARGIGLDVEEVQEAIGRIARLEPRP
                     GRAFLSDNNQYILPEVFVQKSGDDFTVTTNNEQIPHLRISNTYKDLMAQSGNSSEVLN
                     YIREKIRAGKFLIKSLHQRQQTILNIGIEIVKRQREFMEKGVACLKPLTMVQVAEVVG
                     VHETTVSRAVSGKYMQTPQGIFEMKYFFTAGIQTASGEGMSNTSVKDMIAEMFKKEDP
                     TKPLSDQEIVKMLQEKGIVIARRTVAKYRSELNILPSNLRKVY"
     gene            3540..4106
                     /locus_tag="VG754_09955"
     CDS             3540..4106
                     /locus_tag="VG754_09955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019493179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF19584.1"
                     /translation="MLNPLLPDNLDWQPRLACDEDIPAMETLIPLSVRALQANCYSVA
                     QMDAAIGTIFGVDRQLIRDGTYFIVEREAQILGCGGWSKRRSLYGSDALRTGPDPELD
                     PKRDKARIRAFFVHPMWARRGIGRNIMMACEKAIVAAGFRGVEISATITGKLLYASFG
                     YKTVEHYELHMAGNLRLPVVRMTKTFQS"
     gene            4109..4576
                     /locus_tag="VG754_09960"
     CDS             4109..4576
                     /locus_tag="VG754_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWF19585.1"
                     /translation="MSEHKATIQWQRGSADFLSGKYSREHTWTFDGGVTVAASASPAV
                     VPVPYANPANVDPEEAFVASVSSCHMLTFIYLAQQQGIQIETYCDNAVGHMTKNEKGV
                     PWVSSIELHPQIIYGGPKLPSPTEEEHLHHLAHDQCFIANSIKTEVTVKRLMK"
     gene            4632..>5398
                     /locus_tag="VG754_09965"
     CDS             4632..>5398
                     /locus_tag="VG754_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF19586.1"
                     /translation="MSVQLTILGSGSNGNCAYLETAETRILIDAGFSARQTRQRLAAI
                     GRAPEGLTGILITHEHSDHINGLVALCKKINVPIYCNRETRDAMEFYLKAKFDCRIFA
                     TGASFEVGDIVVDTFSIPHDAQDPVGFLLRTSGGNIGFLTDLGHATKLVLQRVRDANV
                     LVLESNHDLKMLQESSRPWSLKQRIAGRHGHLSNSDAADAAEEIMSAELRHLYLGHLS
                     RECNNPELAHRVMQERLQKIGAKHVALELTAQNKPCST"
BASE COUNT         1217 a         1482 c         1389 g         1310 t
ORIGIN      
        1 ttcagttcat attgtatcag atgattttgg aagttgtaaa atatggggac ccggtcttga
       61 gggcaaaagg cgcgcgcatc gaaactatca cgcctgcgat tggccgcttg attgatgaca
      121 tgttcgaaac gatgtaccac gcgcggggca tcggccttgc cgcgcaacaa gtcggtcacg
      181 cgctgcaact gaccgtcatt gatgtccgtg aagccgtgga ccggccttcc acgcttgaac
      241 tggatggcaa gcctgccgag ccaaacgatt tcatgccgct ggtgcttatc aatccccaag
      301 tcaacgccgt caagacttcc gcgaaggcgg agggaccgga aggttgcctg agttttcccg
      361 aaatttttgc cgacatcacc cggcctgaat cggtggacgt agtcgcgatg gacagggacg
      421 ggaagcgcgt tgaatttcgc tgcggcggat tgctcgcgcg ggcggtccag cacgaaacgg
      481 atcatctaaa cggcatcctt ttcattgatc ggatggattc cgaaacaaag agtaatttga
      541 aaccggaaat cgaagacctc caagcagcaa ccaaggccgc gttgcagaag aaaaagaaaa
      601 tgccgccggt gctttaggcg gtcacaaatt tttatatttc catcatttcg ccggagcggc
      661 tggtgcgttt gtcttggact ggcgctcaag ttcacggagt cgcatttggc acgaagttgg
      721 aggcttctcc ggcaaagaat ccaagatgcc gcgaatctct tccggaactt tgatgttgtt
      781 caattggtaa taggccttga tcatccaaag cgcattggtc gaaccggcat tatttctgaa
      841 cgcttgatgc accgccaata cagtttgagg aaaattggat tctatgtttg agaccaaggc
      901 catttcctgg ctcgcgcgga ttctcatcgc aatatttgcc tggtccatga cgctggccag
      961 atacattgtt tcgaacactt ctttcgttgg cttcagcgca acaaaccaca aagtcaaggc
     1021 accgaggata aaacctgcaa gggcaacaag agccattttg aatttcataa aggtaatgca
     1081 gtgccgtttc tctgcagcaa aactgtctaa aggttaatct gagtgcaagc gaaatacatg
     1141 gcgcgcattg ccagactcgg gcacctttcg gttcctgcac tccaagactc caatgcagat
     1201 caatacaact tccgcagatt cgatggtgaa acgatttagt aaacgaggtt tgcgccaaaa
     1261 tctggcattc ggccagcttt cacatcgctg ctccactgat ccagtttact aaaattcttg
     1321 gaccaaaatt tggagcaaag gttaaaatac tcaagcacag tgtccttctc tcctttgagc
     1381 aaaaggtcct gtgccaggct catgttcgga ccaaagctgt ccatctgtgg agatcccgga
     1441 cttttgcctg cggcaagcag gaagtttttg gcctcatcaa tttttccttc ctttacagcg
     1501 atccggccga gaaccacatt tccgtcatgt atcgcgttgc cataattcca gttgccttgg
     1561 aacttcttgg caagcgctaa taaattggtg gcatatttgt gggcgtcatc aattttgtcc
     1621 acctcgaagc tctccttagc ggcatcgttc aacgcgtaga atctttcttc gtcagtggat
     1681 gcttggttga gctttttaat tgcttccgtc agccgtcctt ggggcgtcgt gggaacgctg
     1741 tttccacagc cacaaagcag tgcgcacaaa aacgcgaaag ataggtattt cataaatggt
     1801 tgcatcttcg actccgataa cgccaacgcc ccgcccttca gttctcctcc tgcccagcct
     1861 caataaacct tccttaaatt cgatggtaaa atattcaact cgctgcgata tttcgccaca
     1921 gtgcggcggg cgatgacgat gcctttttcc tgaagcatct tgacgatttc ctggtcagac
     1981 agcggtttgg tcgggtcttc cttcttgaac atttcggcga tcatgtcctt gacgctggtg
     2041 ttggacatgc cttcgccgct cgcggtctgg atgccagcgg tgaaaaaata tttcatctcg
     2101 aaaatcccct gcggcgtctg catgtatttg ccggaaaccg cccggctcac cgttgtttca
     2161 tgcacgccca ccacttcggc cacctgcacc atcgtcagcg gcttgagaca cgccacgccc
     2221 ttttccatga attcccgctg ccgcttcacg atctcgatgc cgatgttcaa gatcgtctgc
     2281 tgccgttgat gcaggctctt gatcaaaaat tttcccgcgc ggattttttc gcggatgtaa
     2341 ttcaacacct cggaagaatt gcccgattgc gccatcaggt ccttgtaggt gttgctgatg
     2401 cgcagatggg gaatttgctc gttgttggtg gtgaccgtga aatcatcgcc ggatttctgc
     2461 acgaaaactt ccggcaaaat atattggttg ttgtcgctca ggaaggcacg gccggggcgg
     2521 ggctccaaac gggcgatgcg gccaatcgcc tcctggactt cttcgacatc caaaccgatg
     2581 cctcgggcga tttccgggat gcgccgcttg cccagcgcgt ccatgtaatc cttcacgatg
     2641 cggtattcga ccgatttttc gtcgcaagcg cgttccagtt ggcgcaggag acattcctgc
     2701 aaatcgcgcg cgcccacacc cggcgggtcg aaggtttgaa ccacccgcaa caccgcctcg
     2761 attttctccg cgggaaggtt cgtcgagaac gccagttcct cgatgctcgt cttgagataa
     2821 ccgtagtcgt caatgttgcc gatgagcatc tcggcaatgg ggcgttgttc gtcgttcagg
     2881 cgcgagtcgc gcacctgctc cagcaagatt tcctgcaatg acgttgcggc gacgatggaa
     2941 tcgaacatga actgccgttt ttcctcgtcc tccgccgatt ggcgcatcgg gatgttcgtg
     3001 gcggcaaagt ggtcgcgcca atcctggtcc atctgggcca gtttctcgaa ttccgccatg
     3061 aaatcgtccg ccggttcgcc atttttctcg gtggtgttgt caaaggtgac gtccgaaggc
     3121 ggttcagcag gatcggatga ttcggcagag tcggtttccg ggccgtcgcc gtcctccttg
     3181 gaatccttct cagactgctc ggcctcgggc gtgggggcct cttccaagac cgggttttgt
     3241 tgcaattcct gttcaaccaa cgcctttaac tccagtgtgg gcgcctgaag caatgccagg
     3301 gactgttgca tctgcggcga cagcaccagc gactgcgtca tgcgctgcga taaatgtaag
     3361 ccttgagcca tgcggggacg attattccca gaatcccgtc caaagctcaa ggaaaattgg
     3421 tctgatttgt gctttagcta ttctgatgcc ccggagaaaa actggtgagt tttccagtca
     3481 gaccattcat tcgtcaactc attatcgcaa aaagatgtga atcgtgttat tccctgccaa
     3541 tgttaaatcc gctgcttccc gataatctgg attggcagcc cagattggcg tgtgacgagg
     3601 acattccagc aatggaaacg ctcatccctc tatccgttcg tgcgcttcaa gccaactgtt
     3661 attccgttgc gcaaatggat gctgcaatcg gtacgatatt cggtgttgat cgccagctca
     3721 ttcgggatgg aacttatttt atcgtcgagc gcgaagcaca aattttaggt tgtggcggat
     3781 ggagcaaacg ccgctctctt tatggaagcg acgccctccg aactgggccc gatccggaac
     3841 ttgacccgaa gcgcgacaaa gcgcgaatca gggcattttt tgtgcatccg atgtgggcgc
     3901 gacgcggaat cgggcgcaac atcatgatgg cttgtgaaaa agcgattgtc gcggcgggct
     3961 tccgtggagt ggaaatttca gccacaatca cgggcaaact gctttacgcc tcatttggtt
     4021 acaaaacggt cgaacattat gaacttcaca tggctggcaa tctgcgtttg cctgtggtga
     4081 ggatgaccaa aacattccag agctaaatat gtctgaacac aaagcgacca ttcaatggca
     4141 gcgcggcagc gcggattttc tcagcggaaa atattcgcgc gaacatacgt ggactttcga
     4201 cggcggcgtc accgtggctg cgtcagcatc gcctgccgtt gtgcccgttc cttacgcgaa
     4261 tccggcgaac gttgaccccg aggaggcgtt tgtggcgtca gtttcaagct gtcacatgct
     4321 gacgtttatt tatctggcgc agcaacaggg cattcaaatc gagacgtatt gcgacaatgc
     4381 agtcgggcac atgacaaaaa acgaaaaagg cgtcccgtgg gtgagttcaa tcgaactcca
     4441 tccacaaatc atatatggcg gccccaaact tccctcgccg accgaagaag aacatttgca
     4501 tcacctggcg catgaccaat gtttcatcgc caattccatc aaaaccgagg ttacggtaaa
     4561 gcgcctcatg aaataagttt tcctgccaaa aatgaattgc tcgcaaagtc gtctctcttt
     4621 accgttgcgg cgtgtccgtt caattgacga ttctcggcag tggttccaac ggcaattgcg
     4681 cctacttgga aaccgccgag acacgcatcc tcattgacgc cgggttcagc gcgcgccaga
     4741 cgcgtcaacg cctcgcagcc atcgggcgcg cgccggaagg cttgaccggc atcctcatca
     4801 cgcacgaaca ttccgaccac atcaacggcc tcgttgcgct ctgcaaaaaa ataaacgtcc
     4861 ccatctattg caaccgggaa acgcgcgatg cgatggaatt ttatttgaag gcaaaattcg
     4921 actgccgcat cttcgcgacc ggcgcgagtt ttgaagtcgg cgacatcgtg gtggacacgt
     4981 tcagcattcc gcacgatgcg caagacccgg ttggattttt gcttcgcact tcgggtggca
     5041 acatcggttt cctgactgat cttggccatg cgaccaagct ggtgctgcaa cgcgtccgcg
     5101 atgccaacgt gctggtgctc gaatcgaacc acgatttgaa gatgttgcag gaatcgagcc
     5161 gtccctggag cctcaagcaa cgcatcgccg gacgccacgg ccatctttcc aattccgatg
     5221 ccgccgatgc cgccgaggaa atcatgtccg ccgaactccg ccatctttac ctcgggcatc
     5281 tcagccgcga gtgcaacaat cctgaactcg cgcaccgcgt catgcaggaa cgcctccaaa
     5341 aaatcggcgc gaaacacgtt gcgctcgaac tgactgccca gaacaaacct tgttcgac
//
LOCUS       DASZMM010000415         6705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1566683, whole genome shotgun sequence.
ACCESSION   DASZMM010000415 DASZMM010000000
VERSION     DASZMM010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566683"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VG754_09970"
     CDS             complement(<1..880)
                     /locus_tag="VG754_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19587.1"
                     /translation="MDDNTMECLRSWMEQNYLGAIQILFGVASPEDPVCELVRRLIAE
                     FPKSDAQLVLCRENLGVNGKVSSLIQMQRLAKHDVIIVSDGDVRVPRDFLTNAVAPLR
                     DAEVGLVNCFYRLANPVNAAMRWEALAVNADFWSQVLQGKDLAPLDFALGAVMITRRE
                     ELAKIDGFEALADHLADDFQLGNRVVRMTGKRIALCPVVVDCLSAPMNWSEAWVHQLR
                     WARTIRVSKPLPYAFSILSNPTFWPLLWALALPSKWSLLTLAVCLLVRMISAQSLQTR
                     LGAVAGQGKYFWLAPVK"
     gene            1194..2249
                     /locus_tag="VG754_09975"
     CDS             1194..2249
                     /locus_tag="VG754_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19588.1"
                     /translation="MPERLRCAVIGTGGIGLDHLTSLSQCFRAVAVAVAESNTARAKE
                     ATERFKIPRSYVDYRELLDQADIDAVTIALPNHLHAPVAIEALKARKHVFLEKPMAMN
                     AREASKIIDTAMKMKRTLMVGQNFRFNKATQMAKMLIQRGDLGELYHARCFWLRRSGI
                     PRIGSWFTQKKLSGGGATFDIGVHVLDTCLHLLGEFDVASVSGQTHSRFGGRGLGEFN
                     WGRSEIDPAKPFDVEDYGVALLKLKSGRTVNFEVSWAAHQPPDGREYGMDLLGTQGGL
                     ALYPARLFRNGATGYETVHLASLKVPQPEDRMHHFVNCVLDGKKPLVTLEESLKVQQV
                     LDGIYASAASGREVRMG"
     gene            complement(2607..2897)
                     /locus_tag="VG754_09980"
     CDS             complement(2607..2897)
                     /locus_tag="VG754_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19589.1"
                     /translation="MACKWLKKLEKRALCGDIPRMVTLKADDRSRIKIPGAKPGQVFA
                     FEDQGNGVRILTEVKAEPKEPFPPGSLRKLVDEMNREWAGVKPKVPVPEDRD"
     gene            complement(2910..4127)
                     /locus_tag="VG754_09985"
     CDS             complement(2910..4127)
                     /locus_tag="VG754_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF19590.1"
                     /translation="MKIFAILRVAARALKRNKMRTILTMLGMIIGVGAVIATVSLGDG
                     AKSQVEAQIASMGQNVVQVFAGSFTRGGVRSGWNNAGTLNVEDALAIEREIPDVTVIS
                     PEYRSGTLVAAGNQNWSTTIMGEGPDYLDIRQWPLAEGSMFTPMDVRAASKVAVVGKT
                     VADQLFPDGNVIGQVIRIKNVPFVVVGELEPKGFSFMGSDQDDTVIVPYSSAMKRLFG
                     ATNIRGIMVQAESSQSMASVTNEITELLRQRHKIAPGHDDDFMLRTQDDIRQIADSAS
                     ATMRILLAGVACVSLIVGGIGIMNIMLVSVTERTREIGIRMAVGAHGWDILMQFLIEA
                     VALSLVGGLLGILAGAGGSKLFAWWKDWPALTSPFWIMGAFVFSALVGIFFGFYPAWK
                     ASKLDPIDALRYE"
     gene            complement(4124..4885)
                     /locus_tag="VG754_09990"
     CDS             complement(4124..4885)
                     /locus_tag="VG754_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF19591.1"
                     /translation="MNAIIELDHVHKIYHTGEVDVHAVRDVSLQIQRGEFVAIMGASG
                     SGKSTMMNILGCLDRPTKGRYLLDGIDVSRLDRDELATIRNEKIGFIFQGFNLLSRTS
                     ALENVELPMMYMRQSVGGRDQRERALRALATVGLADRAGHHPNQLSGGQQQRVAIARA
                     LVNQPAMLLADEPTGNLDTQTSIEIMGVFQKLNDQGITIIMVTHELDIASYTKRNVIM
                     RDGRIVSDLPVANRSIATEELKKLREAHEAVQLVP"
     gene            complement(4890..6158)
                     /locus_tag="VG754_09995"
     CDS             complement(4890..6158)
                     /locus_tag="VG754_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF19592.1"
                     /translation="MILGLAAILAGGGYWYWKHHAESDPEYKTEPVTRGDLVQIVTAT
                     GQLNPRTNVEVGCQVSGIIKKIYVDFNSPVTNRQVIAQLDPITYQTLVEQARADLANA
                     KAALELAKANEDRSAILFTNKIVAQADQDTALASYHQADAQVQLKQAALSQAEANLSY
                     TTIYSPVDGIVLSRNVDVGQTVAASLSSPTLFMIANDLTKMQIDAFVSEADVGGVETN
                     QMVNFTVDAFPTRTFQGKVVQIRNAPQTNQNVITYDTVIGVDNSDLKLRPGMTANVSI
                     ITAQRNNALKIPNGALRFHPPQSNEARKQAGLAAAAASNGAAFAKQVAGAGASGQGRP
                     RGGAGGSGAHPKTEKVIHTVYVLEKGADGKAETLKPVQIKTGINDGIDTQVLDGLKEG
                     DEVVVGQVLANGVVQTSGGSNPFGGGGRRF"
     gene            6254..>6705
                     /locus_tag="VG754_10000"
     CDS             6254..>6705
                     /locus_tag="VG754_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWF19593.1"
                     /translation="MLNLFKNQLAKSVHLTDEQVALAVAQLVDESVPAELKADFLCEL
                     ARKIETPTEIAAFARELRAKCIVPPLDAQTRSGVILDVCGTGGDKLNTFNISTTVAFI
                     CAAAGVTVAKHGNRAVTSQAGSADVLEALGVPVELTPEQAAKSLREHH"
BASE COUNT         1435 a         1953 c         1838 g         1479 t
ORIGIN      
        1 ctttcaccgg cgcgagccag aaatattttc cttgcccagc aaccgcgccc aagcgcgttt
       61 gcaaagattg cgctgaaatc atccgcacca gcagacacac ggccaatgtg agcagtgacc
      121 attttgatgg cagcgcgagc gcccaaagca acggccagaa tgttgggttg ctcaggatgc
      181 tgaaggcgta cggcaacggt ttgctcacgc ggatggtgcg cgcccatcgg agttgatgca
      241 cccatgcttc gctccagttc atcggcgcgg ataaacaatc caccacgacc ggacagagcg
      301 cgatgcgttt gcccgtcatc cgcacgacac gattgccgag ttgaaaatcg tccgccagat
      361 ggtcggccaa cgcctcgaac ccgtcgatct tcgcaagttc ctctcgccgg gtgatcatca
      421 ccgcgccgag cgcaaaatcg agcggcgcga ggtcttttcc ctgcaagacc tggctccaaa
      481 aatccgcgtt caccgccagc gcttcccaac gcatcgccgc gttgacagga tttgccaggc
      541 gataaaaaca attcaccaag ccgacctccg catcgcgcaa cggcgcgact gcattcgtca
      601 aaaaatcgcg cggcacacgc acatcgccat cgctcacgat gatgacatcg tgtttggcga
      661 gccgctgcat ttggatgagc gacgacacct ttccattcac gcctaaattt tcgcggcaga
      721 ggaccagttg cgcatcagat ttgggaaatt cggcgatcaa tcgccgcacc aactcgcaaa
      781 ccgggtcttc cggcgaagcc acgccaaaga ggatttgaat cgctcccaag taattctgtt
      841 ccatccagct ccgcaagcat tccattgtgt tgtcatccaa gcctttaagc ggttttaaca
      901 gcgtcacagc cggtgagaag ttcacctctg taacgcgctg atgaagtggg aaacgcaacg
      961 ctgcaaccca ttgccaaaga agcagtaaca ggctgaggag ggccaaaatc gtcagggcga
     1021 tgaacacccc acgttcctag accgagttga agcgttgggc aagcgtcaat ttaggcgtta
     1081 tttgagcatt ttgtcacaat ccgattgacc agaggtcatc aaattggtta aaacgctttg
     1141 caacccagtc ttattgaggg gatttaaaac aggaattaag ccatcacaaa actatgcccg
     1201 aaagacttcg ttgcgctgtc atcggaacgg gtggcatcgg actggaccat ctgaccagct
     1261 tgtcgcagtg ttttcgcgcc gtcgcagttg ccgttgccga gagcaacact gcgcgcgcca
     1321 aggaagcgac tgagcgtttc aaaattcccc gcagttacgt ggattaccgc gaactgctcg
     1381 accaggcgga catcgacgcc gtcaccatcg ccctgccgaa tcatcttcac gcgccggttg
     1441 caatcgaggc gttgaaggcg cgcaaacatg tcttcctcga aaagccgatg gcgatgaacg
     1501 cccgcgaagc gtccaagatc atcgacacgg cgatgaagat gaaacggacg ctgatggtgg
     1561 gccagaattt ccgtttcaac aaggcgacgc aaatggccaa gatgctcatc cagcgcggcg
     1621 accttggcga actttatcac gcgcgctgtt tttggttgag gcgcagcggc attccgcgca
     1681 tcggttcgtg gttcacgcaa aagaaattgt cgggaggcgg cgcgacgttc gacatcggcg
     1741 tgcatgtgct cgacacctgt ttgcatctgc tgggcgaatt tgatgtcgca agcgtttccg
     1801 ggcagacgca ttcgagattc ggcggacgcg gcctcggtga attcaattgg ggcaggagtg
     1861 agattgatcc ggccaagccg ttcgacgtgg aggattacgg cgttgctttg ctcaagttga
     1921 agagcggacg cacggtgaat tttgaggtga gctgggcggc gcatcaacca cccgacggcc
     1981 gcgaatacgg catggatttg ctgggaacgc aaggtggtct ggcgctttat cccgcgcgct
     2041 tgtttcgcaa cggcgcgacc ggctacgaaa ccgtgcatct tgccagcttg aaagttccgc
     2101 aacccgaaga ccgcatgcat catttcgtca actgcgtgct cgacggcaaa aagccgctgg
     2161 tcacgttgga ggaatcctta aaggtgcagc aggtgctcga tggcatctat gcgtccgcgg
     2221 ccagcggcag agaagtgcgg atggggtgag gcttaaatag ttaaatcgtt acaagttttt
     2281 agtcccttgc tgcgagccgg aaaatgtccc ggattttgtc tctgatcgcg tttcgccgcg
     2341 ccgggctgac catgcctcga taaccgtaga ggctcgatgt ctccaccagc cacatttggg
     2401 aacaaacagc gaacgtttcc cagtccaagc cgtccgactg atccagcagc acttcgtcga
     2461 gctttggtgg ccggttctgg cgctgacttg tgcaataaag gacgttaacc agcgcggccc
     2521 gcgccgaaat gtccgggtgc gaaatcagga tgcacgggtg ttcccctttt ttcggaaatt
     2581 gtatcgcgag actgcgaatt gggttttcag tcccggtcct ccggcaccgg caccttgggt
     2641 ttgactccag cccattcgcg gttcatctca tccaccaatt ttcgaaggct cccgggagga
     2701 aatggttcct ttggttcagc cttgacctcg gtaagtatgc gaacaccatt tccctgatcc
     2761 tcaaaagcaa aaacctgtcc gggctttgca cccgggattt ttatcctgct tcgatcatcg
     2821 gcttttaaag tcaccatgcg gggaatatcc ccgcataaag ctcgtttttc aagctttttc
     2881 agccatttgc aagccatcaa ggagtcaacc tactcatacc gcaacgcatc tatcggatcc
     2941 agcttcgatg ctttccatgc cggatagaag ccgaagaaaa ttcccaccag cgcgctgaac
     3001 acaaacgcgc ccatgatcca gaacggcgag gtcaacgcgg gccaatcttt ccaccaggca
     3061 aataattttg agccgcccgc gcccgcgaga atcccgagca atcctcccac caggctgagc
     3121 gcgaccgcct cgatcaaaaa ttgcatcaag atgtcccagc catgcgcgcc gaccgccatg
     3181 cggatgccga tttcgcgcgt ccgctcggtc acgctcacga gcatgatgtt catgatgccg
     3241 atgccgccga cgatgagcga cacgcacgcc acgcccgcaa gcaaaatgcg catggtggct
     3301 gaggcgctgt ccgcgatctg ccggatgtcg tcctgcgtgc gaagcatgaa atcgtcatca
     3361 tgcccgggtg caatcttgtg gcgttggcgc agcagctcgg tgatttcgtt cgtcacgctt
     3421 gccatggatt gcgaactttc cgcctgcacc atgatgccgc gaatatttgt cgcgccaaac
     3481 aaccgtttca tcgcgctgct gtaagggacg atcacggtgt cgtcctggtc cgagcccatg
     3541 aaggaaaaac cttttggttc aagttcgccg acgacaacga acggcacgtt cttgatgcgt
     3601 atcacctggc cgatgacgtt gccatccgga aaaagctggt cggcgaccgt tttgccaacg
     3661 accgcgacct ttgatgcggc gcgcacatcc atgggcgtga acatgctgcc ctcggcaagc
     3721 ggccattgcc ggatgtcaag gtaatcgggt ccttcgccca tgatggttgt ggaccaattt
     3781 tgattaccgg ccgcaaccaa agttccgctg cgatattccg ggctgatgac ggtgacatcg
     3841 ggaatttcgc gctcgatggc cagagcatct tcgacattca atgtgccggc attgttccat
     3901 ccgctgcgca cgccgccgcg ggtgaaactg cccgcaaaaa cctgcacgac attctggccc
     3961 atgctggcga tttgcgcttc tacctgcgac ttcgcgccgt cgcccaggct cactgtggcg
     4021 atcaccgcgc cgacgccgat gatcatgccg agcatggtga gaatggtgcg catcttgttt
     4081 cgcttcaacg cgcgcgccgc gactctaagg atggcaaaaa ttttcatggc accaattgca
     4141 ccgcttcgtg cgcctcgcgc aattttttca attcctcagt ggcaatcgag cgattggcga
     4201 cgggcaaatc gctgacgatc cgtccgtcgc gcatgatgac gttgcgttta gtgtagctgg
     4261 cgatgtccag ttcatgagtc accatgatga tggtgatgcc ctggtcattg agcttttgaa
     4321 aaacgcccat gatttcaatg ctcgtttgtg tgtcaagatt gccggtcggt tcgtcggcaa
     4381 gcagcatcgc gggctggttc accagcgcgc gggcaatggc cacacgctgt tgctggccgc
     4441 cggaaagctg gttgggatga tgcccggcgc ggtccgcaag accgactgtt gccagcgcgc
     4501 gcaaagcccg ttcacgttgg tcgcgtccac ctaccgactg gcgcatatac atcatcggca
     4561 actcgacatt ttccaaagcc gacgtgcgtg aaagcaggtt gaagccttga aaaatgaaac
     4621 caattttttc gttgcgaatg gtggcaagtt cgtcgcggtc gagtcgcgag acatcgatgc
     4681 cgtcgagcag gtaacggcct tttgtcgggc ggtcaagaca gccgaggatg ttcatcatgg
     4741 tggatttgcc cgagccgctg gcgcccatga tggccacgaa ttcaccgcgc tggatttgca
     4801 gcgagacatc acgcacggcg tgaacatcca cctcgccggt atggtaaatc ttgtgaacgt
     4861 ggtccagctc gatgatggcg ttcatggaat taaaaccgcc gtccaccgcc gccgaatgga
     4921 ttggagccgc cgctggtttg caccacgccg ttggcgagaa cctgccccac caccacttca
     4981 tcgccttcct tcaaaccatc aagcacctgg gtgtcaatgc cgtcgttgat gccggtcttg
     5041 atttgcaccg gcttcaaggt ttcggctttt ccatctgctc ctttctccaa cacatatacg
     5101 gtatggatga ctttctcggt tttgggatgc gcgccgctgc cgcctgctcc gccacggggc
     5161 cgaccttgtc ccgacgcacc cgcgcctgcg acctgtttgg caaaagccgc accattgctt
     5221 gcggcggccg cggcgaggcc tgcctgtttc ctggcctcgt tggattgcgg cggatgaaac
     5281 cgcaacgcgc cgttcggaat cttcaatgcg ttgttgcgtt gcgcggtaat gatggaaaca
     5341 ttcgctgtca tgcctgggcg caatttcaaa tcggagttgt ccacaccgat aacagtatca
     5401 taagtgatga cgttttggtt ggtttggggc gcattgcgga tttgaaccac ctttccctga
     5461 aatgtcctcg tcggaaaagc gtccaccgtg aaattcacca tttggttcgt ttccacgccg
     5521 ccgacgtcgg cctccgagac gaatgcgtca atctgcattt tcgtgaggtc attggcgatc
     5581 ataaacaacg tcggcgaact gagactcgcg gcgacggttt gtccgacatc cacattgcgc
     5641 gaaagaacga tgccgtccac cggcgaataa atcgtggtgt acgagaggtt cgcctcggct
     5701 tgactgagcg cagcctgctt gagttgcact tgggcatcgg cttgatggta tgaggccagg
     5761 gcggtgtcct ggtccgcttg cgcgacaatc ttgttcgtga aaagaatggc ggaacggtcc
     5821 tcgtttgctt tcgccagttc aagggcggcc ttcgcattgg ccaggtccgc gcgcgcctgt
     5881 tcaacaagcg tttggtaagt tatgggatcc agttgcgcga tgacctgccg gttggtcacc
     5941 ggggaattaa agtccacata aatttttttg atgatgccgg aaacttgaca gccaacttcc
     6001 acattggttc gcggattcaa ctgcccggtg gcggtcacga tctggaccaa gtcgccgcgc
     6061 gtcacaggtt cggttttgta ttccggatcg ctttcggcgt gatgcttcca ataccaatag
     6121 ccgccgcccg cgagaattgc ggccaacccc aaaatcatca accaacgaac aacaccaaac
     6181 ccactattct ttgccatcat attgctaaaa cttatgcttg ggcctgcggc ccttccgcca
     6241 ttagactgcc aacgtgctga acttatttaa aaatcagctc gccaaatcgg ttcacctgac
     6301 cgacgagcag gtcgcgcttg ccgtcgcgca actcgttgat gaatcggtcc cggcggaact
     6361 taaggccgat tttctctgcg aactggcccg caaaatcgag acaccgacgg aaatcgccgc
     6421 ttttgcacgc gaattgcgcg caaaatgcat tgttccaccg ctcgacgccc aaacccgctc
     6481 cggcgtcatc ctcgacgtct gcggcaccgg cggcgacaag ctcaatacat ttaacatctc
     6541 gacaactgtc gcgttcatct gtgccgcggc gggagtcact gttgccaagc atggcaatcg
     6601 cgcggtcact tcgcaagccg gcagtgcaga cgttttggaa gcgctgggcg tgccagtcga
     6661 gcttacgccg gaacaggcgg cgaaatcatt gcgcgaacat cattt
//
LOCUS       DASZMM010000416         5249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1304274, whole genome shotgun sequence.
ACCESSION   DASZMM010000416 DASZMM010000000
VERSION     DASZMM010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304274"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1941)
                     /locus_tag="VG754_10005"
     CDS             complement(<1..1941)
                     /locus_tag="VG754_10005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF19594.1"
                     /translation="MHRLFLRNNITINGGEHVIAKPWRNCTTKSEFFKVGGNPIFAHR
                     AKSHTHAERVDYQKEYEEYWSRPDRWGSHSFRNPDLIADQIASLCGTGKILDVGFGMG
                     LLVKTLLARGIDACGMDIAECVVREANSFAPGHFTAGSMLAMPFPDGAFETIVSTDCM
                     EHIAEADLPKVLAEFYRVAQRYLFIRLATTPDRDKRWHLTIRNRTWWEQQFFQAGFRK
                     HPAIQTILPYESLETENWQITLAFEKIPPAVLDQFPLSSLKPERDLHMDMLRESGRRS
                     DAHIARYMLARQFLPKEGLVLDAACGLGYGSAILAQSSPSVRVIGMDSSAFAVNYARA
                     NFGGSWANVEFCEGDVNHLRNIADNSVSLVASFETIEHLREPELFLAEVQRVLQPGGV
                     FLSSVPNLWVDETGKDPNPHHFHVFDFPKLAVLCRKFFKLREVYRQTAGGGMKLPNAP
                     RELRRVNLPVTSQTEHAEWWLVAAEKEPAVSIQPPSKNGSHSNSKGELILLTSDPTHP
                     FYTSWLPALDRPWRALNPAHADLNLLRDAGLIVTHDTYVDPGRKLAREVFGAGIPTLI
                     LADGILEYRNTWEHPKNEPGAIFQPVLGHKIACLGRSQARILESWGNIGKCEIVGSPR
                     FDGYCELQRRQKRPDEPFRILVM"
     gene            1964..2872
                     /locus_tag="VG754_10010"
     CDS             1964..2872
                     /locus_tag="VG754_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWF19595.1"
                     /translation="MSFIIFASPKSGTTWLQRLISRHPAALCAESRPFGDYYAATSAT
                     GPHLTMDRFCGILSDYFAPSVPELKQEDMPFRRALAHNFLDTLAASALAASKKLVYGE
                     KFTPYRGTALRAIAALEEYHPKIKFVNLTRDGRDVIASGAAHWLNLRLRKAKPEERPR
                     FEEALRNHTILQEDFDMFLDCWMDAVQAGLAACRRFPNYLRIRYEDFVQEPCGQTAKL
                     FDFLGLKSSKAAVRGCVEATSFNQLSQGRKRGEEDLSSFFRKGIVGDWKNWFTSGQLA
                     SFEETAGSFLAELGYPREKPGVCQPV"
     gene            2942..3706
                     /locus_tag="VG754_10015"
     CDS             2942..3706
                     /locus_tag="VG754_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19596.1"
                     /translation="MKLLRQMSPETNSNSNALARKIGATPLRTVPFGHIYHEQIFPMR
                     LYREILRQLPDSRFYDELRHPDALQPDGSSARLSFNLYSENIRRLPVMQREFWRMLTR
                     EMTAPEVEAAFKAKFKKILEEHCQRKLEKIKLRPWPMLLRDIAGYQISIHPDVPQKAI
                     TTQYYLPEDENQKQLGTVLHERLPGGGFNEICTLPFLPDSGYAFPVTTESWHSVKVMN
                     TNGPPRNSLMVTYFYDRGWVVEGFRKLVRRLREMRH"
     gene            complement(3761..4204)
                     /locus_tag="VG754_10020"
     CDS             complement(3761..4204)
                     /locus_tag="VG754_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF19597.1"
                     /translation="MQTRASIPINFREARERAGLPPEEAARRMCVSRSMLRNLESEDD
                     ELTAVHSPADVQRFCSVLGVRPAELLEVKCSSKPLTPEEIAATIFEHCQKKGMNVSHF
                     EDTVGWCVARSLEEPRRFLHDYSLDALHDICRELNLEWERLVAGL"
     gene            complement(4442..4774)
                     /locus_tag="VG754_10025"
     CDS             complement(4442..4774)
                     /locus_tag="VG754_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19598.1"
                     /translation="MRVDGPEGFISVHIYPESSKPDEAMEGLRQFDVGKPVLIEETFP
                     LSCNAVQLENFMRASRVIATGWVGHYDGESLAELDALDRARKISATQALYRAWLQLFV
                     RLAPDFAG"
BASE COUNT         1183 a         1458 c         1447 g         1161 t
ORIGIN      
        1 catcacaaga atgcgaaacg gctcgtccgg gcgtttttga cggcgctgca attcacaata
       61 gccgtcgaag cgcgggctgc cgacgatttc gcatttgccg atgttgcccc acgattccaa
      121 aatgcgcgct tgcgaacggc cgaggcaggc gattttgtgg ccgagcaccg gttgaaaaat
      181 cgcgcccggc tcgttcttcg gatgctccca tgtgttgcga tattccagga ttccgtcggc
      241 aaggatgagt gtcgggatgc ccgccccgaa gacttcgcgc gctaatttgc gtcccggatc
      301 aacatacgta tcgtgcgtga caatgagccc cgcgtcgcgc agcagattca aatccgcatg
      361 ggcgggattg agggctctcc atggtcggtc caatgccggc agccaacttg tgtaaaacgg
      421 atgcgttgga tcggaagtga gcaggatgag ttcgcctttc gaattggaat gcgagccgtt
      481 tttcgaggga ggctgaattg aaaccgcagg ttctttctct gctgcaacga gccaccattc
      541 ggcgtgttca gtttgagagg tgaccggcag attcacgcgg cgcagttcac gcggcgcatt
      601 cggcagtttc atcccgccgc cggcggtttg ccgataaact tcgcgcaatt taaaaaattt
      661 ccggcacaac acggccagct tcggaaaatc gaacacgtga aaatggtgcg gattcgggtc
      721 tttgccggtt tcatccaccc aaaggttcgg cacggaactg agaaacacgc cgccgggctg
      781 gagcacgcgt tggacttcgg caagaaacaa ttccggctcg cgcagatgct cgatggtttc
      841 gaacgacgcc acaagcgaaa ccgaattgtc cgcaatattc cgcagatgat tcacgtcgcc
      901 ctcgcaaaat tccacgtttg cccaagagcc gccaaaattt gcgcgcgcat aattcaccgc
      961 gaatgcgctg gaatccatgc cgatgacgcg gacagacggc gaactttgcg caagaattgc
     1021 gctgccataa ccaagtccgc acgcggcatc gagcacgagt ccttctttcg gtagaaactg
     1081 ccgcgccagc atgtagcggg cgatgtgcgc atccgagcgg cggcctgatt cgcgcagcat
     1141 gtccatgtgc agatcgcgct cgggtttgag gcttgaaagc ggaaattggt cgagcacggc
     1201 gggcggaatt ttctcgaaag ccaaagtgat ctgccagttc tcggtttcga gcgattcgta
     1261 gggcaggatc gtttggatag ccggatgttt gcggaaaccg gcctggaaaa attgctgctc
     1321 ccaccaggtt cgattgcgaa tggtcagatg ccagcgcttg tcgcggtcgg gcgtggtggc
     1381 gagccggatg aagagatacc gttgcgcgac tcgataaaat tccgcgagca ccttgggcag
     1441 gtcggcttcg gcgatgtgct ccatgcaatc ggtgctgacg atggtttcga acgcgccgtc
     1501 gggaaaaggc atcgcgagca ttgaaccggc ggtgaaatgt ccgggtgcaa aactgttcgc
     1561 ctcgcgcacc acgcattcag cgatgtccat cccgcaagca tcgatgccgc gcgccaaaag
     1621 cgttttcacc agcaacccca tgccgaaacc gacatcgagt attttgccag tgccgcaaag
     1681 cgaagcaatc tggtctgcta tcaggtcggg gttgcggaag gaatggctgc cccaacggtc
     1741 ggggcgcgac cagtattctt catattcttt ctggtaatcc acgcgttcag cgtgagtgtg
     1801 tgattttgcg cgatgcgcga aaatggggtt accacctacc ttgaagaatt ctgatttcgt
     1861 ggtgcaatta cgccacggtt tggcgatgac gtgttcgccg ccgttaattg ttatgttgtt
     1921 cctcaaaaaa agccggtgca ttcgggtgtt tcgagcacca cgaatgtctt tcatcatttt
     1981 cgccagcccc aaatccggca ctacctggct gcaacgtctt atttcacggc atcctgccgc
     2041 attgtgcgct gaatcgcggc cattcggcga ttattacgcc gccacttccg ccaccgggcc
     2101 gcacctgaca atggatagat tttgcggcat cctaagcgat tacttcgccc cgagcgtgcc
     2161 ggaattgaaa caggaggaca tgccgttccg ccgcgcgctg gcgcacaatt ttctcgacac
     2221 acttgccgcg agcgcgctgg ccgcgtcaaa gaaactcgtt tacggagaaa aattcacccc
     2281 ttatcgcggc accgcgttgc gggcgatcgc ggcgctggag gaatatcatc ccaaaatcaa
     2341 attcgtgaac ctgacacgag atgggcgcga tgtgatcgct tccggcgcgg cgcactggtt
     2401 gaacttgcgc ctgcgaaagg caaagccgga ggagcgtccc cgctttgaag aagccctgcg
     2461 caatcacacc attttgcagg aagactttga catgttcctc gattgctgga tggatgccgt
     2521 gcaagcggga ctggccgcgt gccggcgatt tcccaattat ctccgcatac gttatgagga
     2581 ttttgtgcag gaaccttgcg gccagacagc gaaattattt gattttttgg gcttgaaatc
     2641 cagcaaggcg gccgtccgcg gctgcgtgga agcaaccagt ttcaaccaac tttcgcaagg
     2701 ccgaaaacgc ggtgaagagg acctcagcag cttttttcga aaggggattg tgggcgattg
     2761 gaaaaactgg ttcacaagcg ggcaactggc ctcctttgaa gagactgctg gctcgttcct
     2821 ggctgaactg ggctacccac gcgaaaaacc gggggtctgt cagccggttt gaccgtcgcc
     2881 aggttgcttt gcattttcac taggcatggg gtaagcacct actggcggaa attttcaatc
     2941 cttgaaactc ttgcgtcaaa tgtcgccgga gacgaactca aacagcaacg ctctcgcgcg
     3001 gaaaatcggg gcgacgccat tgagaacggt tccgtttggc cacatctacc acgagcaaat
     3061 tttcccaatg cggctttacc gggaaattct ccggcagctt cctgacagcc gattttacga
     3121 tgagttgcgg catccggacg ctttgcagcc ggacggcagc agcgcgcgac tcagtttcaa
     3181 tctttactcg gaaaacatcc ggcgcctgcc ggtgatgcag cgcgaattct ggaggatgct
     3241 cacacgggaa atgaccgcgc ccgaggtcga agccgcgttc aaggcgaagt tcaaaaaaat
     3301 tctcgaggaa cattgccagc gcaaattgga aaaaatcaaa ctgcggccct ggcctatgtt
     3361 gctgcgggac atcgccggct accaaatctc catccatccc gacgttccgc aaaaagccat
     3421 cacgacgcaa tattatctgc ctgaagacga aaaccaaaaa cagcttggca cagtgctgca
     3481 cgagcgtctg ccgggtggcg gttttaatga aatttgcact ctgccattct tgccagacag
     3541 cggctacgcc tttcccgtca cgaccgaaag ctggcacagt gtcaaggtga tgaacaccaa
     3601 cgggccgccg cgaaacagcc tcatggtcac ttatttttat gaccgcggct gggtggtgga
     3661 agggttcaga aaactggtgc ggcggctgcg cgagatgcgg cattaaatcg tcgagccggt
     3721 tttccttgta acgatttaac gatgtaacca tttaacgctt ctaaagtccc gccacgagcc
     3781 tttcccattc cagattcagt tcgcggcaga tgtcgtgcag cgcgtcgagg ctgtaatcgt
     3841 ggagaaagcg gcgcggttct tcaaggcttc gggccacgca ccagccgacc gtgtcctcga
     3901 aatgcgagac gttcatgcct ttcttttggc aatgctcgaa aatcgtcgcg gcgatctcct
     3961 ccggggtgag tggcttgctg ctgcacttca cttccagcaa ttcagcggga cggacgccca
     4021 acaccgagca gaaacgttgc acatcggcgg gcgaatggac cgccgtgagt tcgtcgtcct
     4081 cggattcgag attgcgcaac atggaacgcg agacacacat gcggcgcgcg gcttcttcag
     4141 ggggaagccc ggcacgttcc cgcgcttcgc gaaaattgat cggaatcgaa gctctggttt
     4201 gcataacttt cggtttttcc aacttttcaa atcaacctgc atccccgacg cgtggcagtg
     4261 tcaacccgtt gacaggattt tgattgtttt tgatttgctt gctgatggcg cggcgaagga
     4321 ttccaacctc ccaccgaaag gttctcgtac caataacatg tttccgaccg agcgcaaatg
     4381 cgaatccttt tttaacctgg ttgtgacaca tgagtggttt gccccaatac aatcgggcgt
     4441 ttcaccccgc gaaatccggc gcgagtctca caaacaattg caaccatgcc cgatacaacg
     4501 cttgcgtcgc ggaaattttc ctggcgcggt cgagcgcatc cagttccgcc aacgactcgc
     4561 cgtcgtaatg gccgacccat ccggtcgcga tcacgcgcga ggcgcgcatg aaattttcca
     4621 gttgcacggc gttgcatgag agcgggaacg tctcttcgat caagaccggc ttgccgacat
     4681 caaactgccg caagccttcc atcgcttcgt ccggcttgct gctctccggg tagatgtgga
     4741 cgctgatgaa accctccggt ccatcaactc tcaaccatca accattggtg ggggggaggg
     4801 gggtgttttt ggcggaattt tgtgaaactt tatgaaattt tgcggaatct caagtactta
     4861 caacttgagc catttcactc aatttcaaca aaccggatga aaaaaatatt aattttttta
     4921 tcccggttta tcgcggtcaa gcccggttga tctgaaaata actgaaacaa gctgaaacaa
     4981 cgtagctgaa aacgtgagtt ttcaggtcct tccggacggg ccatgttcgg cgaagtccgc
     5041 caaacgtcca agcatgagag gaccgagacg tgacttgccc gcgaaaaaca cgaaagtccg
     5101 cgaaatccgc aaccttaaac cttaaacttt gaaccattcc cggcaaaacc cggaacttgg
     5161 cttcatggtt ttctcgcgaa gaccgccaag tccgcaaagg taacgccacg cactccgtgc
     5221 gcgccgcttt ccttaactcg gaacttggc
//
LOCUS       DASZMM010000417         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1816180, whole genome shotgun sequence.
ACCESSION   DASZMM010000417 DASZMM010000000
VERSION     DASZMM010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816180"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VG754_10030"
     CDS             complement(<1..380)
                     /locus_tag="VG754_10030"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWF19599.1"
                     /translation="MNSTKTRCAWARNELSIRYHDEEWGVPVHDDRTWFEFLILEGAQ
                     AGLSWDTILKKRGRYREVFDQFDPAKVARYGDRKVAQLLADAGIVRNRLKIAAAILNA
                     KAFLVVQKEFGSFDAYIWQFTGGKP"
     gene            516..>2044
                     /locus_tag="VG754_10035"
     CDS             516..>2044
                     /locus_tag="VG754_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWF19600.1"
                     /translation="MRCLKFFPAILILCVASVATLQAQTNEMKTNPITRQTVIEAEKI
                     SGLDFSDAKIDQMLRGLNSDVENYRAIRKFPLPNSVPSAMLFNPIPVGMKWETVRKKF
                     KMSPPGAVKLPENPDDLAFYSIGQLSALIKSRKLSSEQLTRFCLDRLKRFDPQLKCVV
                     TLTEERALEEARRADREIAAGHYRGPLHGIPYGAKDLLATKGIKTTWGTAPYQDQVFD
                     EDAEVIKRLEAAGAVLVAKFSLGELAMGDTWYGGMTRNPWNIKQGSSGSSAGPASATA
                     AGLVPFAIGSETHGSIVSPCERCGVTGLRPTYGRVSRTGAMSLSASMDKLGPIARTVE
                     DCAIVFNAIYGPDGVDQTLYDAPFNYDPKVKWQKLRIGYLADDFEKEKGSSRTNSMAA
                     LEKLRAMGAKLTPVELPKLPVDAIEFVLSVEGAAAFDDLTRSNRDDLMKQQNRGSWPN
                     VFRKARFVPAVEYIQANRIRYQLVQETARALKDFDVLVSPTFAGECHLITNLTGQPCV
                     VV"
BASE COUNT          458 a          614 c          551 g          421 t
ORIGIN      
        1 ggtttgccgc cggtgaactg ccagatgtag gcgtcgaaac tgccgaattc cttttgcacc
       61 acgagaaatg ccttcgcgtt caggatggcc gccgcaatct tgagccggtt gcgaacgatg
      121 cccgcgtcgg caagcagttg cgcaactttg cggtcgccat aacgcgcgac cttcgccggg
      181 tcgaattgat cgaacacctc gcggtaacgc ccgcgttttt tgagaatggt gtcccaactc
      241 aagcccgcct gcgcgccttc caaaatcagg aactcgaacc acgtccggtc atcatgcacc
      301 ggcacgcccc attcctcgtc gtgatagcgg atggacaatt cattgcgcgc ccaggcgcag
      361 cgggttttgg ttgaattcat ataattagcg tgaggaaaat tcgaagcaaa ttcaaaggca
      421 ttttgcagaa tgaactgaat ggaaagaatg agggaaaagg ctccgaatcg caaatctggt
      481 tgcttctttt tcctttcttt gcgccaatat ccgccatgcg ctgcttgaaa tttttcccgg
      541 ccattttgat tctctgcgtc gcgtccgtcg caacgctcca agcgcagaca aacgaaatga
      601 aaaccaaccc catcacccgc caaaccgtca tcgaggcgga aaaaatttcg ggactcgatt
      661 tttcggatgc caaaatcgat caaatgctcc gcggcctcaa cagcgacgtt gaaaattacc
      721 gcgccatccg caaatttccg ctgcccaaca gcgttccttc ggcgatgctg ttcaatccca
      781 tcccggtcgg catgaaatgg gaaaccgttc gcaagaaatt caagatgagt ccgccgggcg
      841 cggtgaagct gcccgaaaat ccagatgacc tcgcattcta ttccatcggc cagctttccg
      901 cgctcatcaa atcccgcaaa cttagttcgg agcaactcac gcgcttttgc ctcgaccgtc
      961 tcaagagatt cgatccgcaa ctcaaatgcg tcgtcacgct caccgaggag cgcgcgttgg
     1021 aagaagcccg ccgagccgat cgcgaaatcg ccgccggaca ttatcgcggg ccgctccatg
     1081 ggattccgta tggcgcgaaa gatttgcttg cgaccaaggg catcaaaacc acatggggca
     1141 ctgcgccgta ccaagaccaa gtctttgacg aagacgccga agtcatcaaa cgcctcgaag
     1201 ctgccggagc agtgcttgtc gcaaagttca gccttggcga acttgcgatg ggcgacacct
     1261 ggtatggcgg catgacgcgc aatccttgga acatcaagca aggttccagc ggttcgtccg
     1321 ccgggccggc ctcggcgact gcggcaggat tggttccatt cgcgatcggt tccgaaacgc
     1381 atggttcgat tgtttcgccg tgcgaacgct gcggcgtgac cgggttgcgt cccacgtatg
     1441 gccgcgtgag ccgcaccggc gcgatgagcc tgagcgcgtc catggacaaa ctcggcccca
     1501 tcgcgcgcac ggtcgaggac tgcgccatcg tgttcaacgc catttacggg ccggacggcg
     1561 ttgaccagac tctctacgac gcgccgttca attatgatcc gaaagtaaaa tggcaaaaat
     1621 tgcgcatcgg ttatctcgca gacgatttcg agaaggaaaa aggatcgagc cgcacgaatt
     1681 ccatggccgc gctcgaaaaa ttgcgcgcca tgggcgcgaa gctcactccg gtcgaattgc
     1741 ccaagcttcc cgtggatgcc atcgagtttg tgctcagcgt ggaaggcgcc gcggcgttcg
     1801 atgacctcac ccggagcaat cgtgacgatt tgatgaagca gcaaaatcgc ggctcctggc
     1861 ccaacgtctt ccgcaaggcg cgatttgttc ccgccgttga atacatccag gccaaccgca
     1921 tccgttacca gctcgttcag gaaaccgcgc gcgcgttgaa agactttgac gtgctcgtgt
     1981 cgcccacgtt cgccggcgaa tgccatctca tcacgaactt gaccggccag ccttgcgtgg
     2041 tggt
//
LOCUS       DASZMM010000418         5837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_164037, whole genome shotgun sequence.
ACCESSION   DASZMM010000418 DASZMM010000000
VERSION     DASZMM010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164037"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VG754_10040"
     CDS             <1..187
                     /locus_tag="VG754_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19601.1"
                     /translation="NYNNQLFINNLLHFYENRVKPSRAIIGMGQAMAAFANTPPLYHT
                     ISLHEYPDHESSLISAA"
     gene            281..2494
                     /locus_tag="VG754_10045"
     CDS             281..2494
                     /locus_tag="VG754_10045"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HWF19602.1"
                     /translation="MKMRIVSVSVGTLGLAVVGLVLLAGRVQGQANAPADSATAEAPS
                     APSLIADNTAPIIPPALPPAPTTVSAAQSAPASPQPAASPVAVPVAPSLPADIKLSKG
                     ATDIARFAQGGITEDVMLAYIGNSKWKFSLGSDQIVYLNDLGVSGSVVKAMIQRDSEL
                     DAMSQAYTASLAAAQQQAQPANPYPYPTAPTDYPENPPAPPMDDTSAYSPPPDPGYVP
                     TDDADYFYSSLAPYGSWVYVAGCGFCWQPTVCVINHEWRPYCDRGRWLFSDCGWYWQS
                     DYTWGWAAFHYGRWFEDAHHGWCWSPDHVWGPAWVSWRASHDNCGWAPLPPSAHYEPG
                     RGFTYAHHPVGAGFEFGLRANQYTYIPIARMTDYSPARYAATSWEADQIHNHTTVITA
                     VSEQNHHIFARGIDPNQVAALAGTTVRHAEVHTVPHNGLQPVQPDRLGKSGGSLVIYR
                     SELPAPTHVSKMGNGVMPASRPAGSTPRNFGQPRTSISTAAPQDVRLAANANASHEAY
                     PPGSLILRGNGEPASRALPNMDLSYLKQQTPTVSQPIAEANNSGTYNYSQRTWQPAQP
                     MRPVRSQSEYSVVNGFARPNTPMHITQLNAPSFSAPSQNSGPSVYVMHGSYTAAQHFS
                     GGSSSPYTVYSSSTPAPAHSGHNEGNNSYAIPGSFTASEHLNASSTQARGTDFSSPRH
                     SHEQQPAQAESHPHFESHAESHTESHAATYSAPAPASHSSSSSSSSSGSSSSGRR"
     gene            complement(2515..2712)
                     /locus_tag="VG754_10050"
     CDS             complement(2515..2712)
                     /locus_tag="VG754_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19603.1"
                     /translation="MSNDHLITLRHTLELPDGSGFESRYVRVGLEAMIELCESRLDFF
                     NSLPDAEERRLRDKCDVEFVL"
     gene            complement(2717..3250)
                     /locus_tag="VG754_10055"
     CDS             complement(2717..3250)
                     /locus_tag="VG754_10055"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HWF19604.1"
                     /translation="MDILTALNRRGSELGLQFLVIGGLAINAHGYERVTSDIDLLVRN
                     EEREAWKSLMASLGYSEHHEQGTFMQFTASEGTTWPVDLMFVNQQTFSKMYPESIEVK
                     MKGMEFRVPALEHILALKLHALKYTHGKRELKDLLDVVWLAEINHIDINGEKFIELCR
                     RYGTERIHQQIIAVSSR"
     gene            complement(3317..4198)
                     /locus_tag="VG754_10060"
     CDS             complement(3317..4198)
                     /locus_tag="VG754_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWF19605.1"
                     /translation="MIVAMGPFVLLSCKEQTTAATPSAHTNQQIFQVKGLVKELKSDG
                     KTVVIKHEEVPHYMPAMTMPFEVRNTNELRGLRSGDLISFQMVVTENDAWIEHVIKLT
                     AARATELPSRPTFRLVRDVDPLQVGDVLPEYHFTNELGQAVSTKQFKGQAFAFTFFFT
                     RCPYPTFCPLMSGNFEAAEKKLLAMPNAPTNWHLFSISFDTEYDSPAQLKAYAGRYDY
                     QPEHWSFLTGDLVDITAIGDQVGEYFGHDESGAVTHNLRTVVVDTRGRIQKIFPDNKW
                     TSDELVAEMLKAAAVKP"
     gene            4327..4770
                     /gene="mntR"
                     /locus_tag="VG754_10065"
     CDS             4327..4770
                     /gene="mntR"
                     /locus_tag="VG754_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HWF19606.1"
                     /translation="MPTSSSPSQSAEDYLERIHELIEEKGYARVVDIASSLKVKQASV
                     TSMVQKLGEAGYLKYEKYRGLILTNKGREVAQNIHDRHETLSRFFSLLGLDAETQRRD
                     IEGIEHHLSPATVEVLADLAGFFEEHPETLRQFQQSRRASKRKGA"
     gene            complement(4778..5245)
                     /locus_tag="VG754_10070"
     CDS             complement(4778..5245)
                     /locus_tag="VG754_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020750015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF19607.1"
                     /translation="MATKPVPDGYHSITPYLVLKGAAEGMEFYKKAFGATECMRMPGP
                     DGRIGHAEMKIGDSPFMLADESKEMDAKSPQTLGGSPISILLYVQDVDAMVKQAVAAG
                     AKLIRPVEDKFYGDRMGTLTDPYGHTWHIATHKEDVAPEEMRKRMEAMCKQKA"
     gene            complement(5310..>5837)
                     /locus_tag="VG754_10075"
     CDS             complement(5310..>5837)
                     /locus_tag="VG754_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HWF19608.1"
                     /translation="AFGGVFNAKGLDLWKLDQFANENKTVVGFPEAEPISNEQLLVTP
                     CDILVPAALERQITKQNAGKIECRILAEAANGPTNPEADAIIEQRDQIFLIPDVLCNA
                     GGVVVSYFEWVQDLQSFFWNETEVTDKLFRILEGAFTQTLALSRKQKMPMRQAALSLG
                     ISRVRDAKKIRGLFP"
BASE COUNT         1297 a         1674 c         1546 g         1320 t
ORIGIN      
        1 aaattacaac aaccagttat tcatcaacaa cttactccat ttttacgaaa accgagtcaa
       61 gccaagtcgt gccattattg gtatgggtca agccatggcc gctttcgcca atactccacc
      121 actctatcac accatttccc tccatgaata cccagaccac gaatcgagtc tcatcagtgc
      181 agcatgaaaa aaccattcgc cagattaatt gtaacttatc gttggagcac ggttcattac
      241 tattgaaaag cgaatgcgcg aacctactgg agagtgactt atgaaaatga ggattgtcag
      301 tgtcagtgtc gggacgctgg ggctggcggt ggtcggtctt gtcctgctgg ccgggcgcgt
      361 ccagggacag gcaaacgcgc cggccgattc ggcaaccgca gaagcgcctt cagcgccgag
      421 tttgattgcc gacaataccg cgccaataat cccgccggcg ttgccacccg caccgacaac
      481 cgtttccgcg gcgcaatccg caccggcaag tccgcaaccc gctgcatcac ccgtcgcagt
      541 tcccgtcgcg ccgtcgttgc ctgctgatat aaaactttcc aagggcgcga cggacatcgc
      601 ccgttttgcg caaggcggca tcaccgaaga cgtgatgctt gcctacatcg gcaattccaa
      661 atggaagttc agcctcggtt cggaccagat tgtttatctg aatgacctcg gtgtctccgg
      721 cagcgtggtc aaggcgatga tccagcgcga ctcggaattg gatgcgatgt cgcaagccta
      781 taccgcaagt ctcgccgccg cgcagcagca ggcgcagcct gccaatccgt atccatatcc
      841 gactgcaccc accgattacc cggaaaatcc gcctgctccg ccgatggacg acaccagcgc
      901 atattcgccg ccgcccgatc ccggttatgt gccgacggat gacgcggatt atttttacag
      961 ttcgctcgcg ccgtacggga gttgggttta tgtggccggt tgcggatttt gctggcagcc
     1021 gacggtgtgc gtgatcaatc acgaatggcg gccttattgc gatcgcgggc gctggttgtt
     1081 cagcgattgc ggctggtatt ggcaatcgga ttacacgtgg ggatgggcgg catttcatta
     1141 cgggcgctgg ttcgaggacg cgcatcacgg ctggtgctgg agcccggacc atgtttgggg
     1201 cccggcatgg gtgagctggc gcgcgtcgca cgacaattgc gggtgggcgc cgttgccgcc
     1261 ctcggcgcat tacgaaccgg gcagagggtt cacctatgcg caccatccgg ttggagcggg
     1321 attcgaattc ggtttgcgcg cgaaccaata cacctacatt cccatcgcgc ggatgaccga
     1381 ttattcaccg gcgcgttatg ccgcgacttc gtgggaagcg gaccaaatcc ataaccacac
     1441 gacggtcatc acggcggtga gcgaacagaa tcatcatatc tttgcgcgcg ggattgatcc
     1501 gaaccaggtt gcggcgctgg cgggaacgac ggttcgccac gctgaagtcc acaccgtgcc
     1561 gcacaatggc ctgcaacctg tgcaaccgga ccgcttgggc aagagcggcg gaagcctggt
     1621 gatttaccgg tcggaattgc ccgcgccaac gcatgtttcg aaaatgggca acggcgtgat
     1681 gcctgcttcc cgtccggcgg gttccacgcc gcggaatttc ggccaaccga gaacgtcaat
     1741 cagcaccgct gctcctcagg atgtcaggtt ggccgcaaac gcgaatgcat cgcacgaagc
     1801 gtatccgccg ggttcgctta ttttgcgcgg caatggcgag cctgcttcga gggcgctgcc
     1861 aaacatggat ttgtcctatt tgaaacagca gacgccaact gtttcgcaac cgattgccga
     1921 agcaaataat tccgggactt acaactactc gcaaaggacc tggcaaccgg cacagccgat
     1981 gcgccccgtc aggtcgcaaa gcgaatacag cgttgtgaac ggattcgcac ggccaaacac
     2041 cccgatgcac atcacgcagt tgaacgcgcc gagtttttcc gcgccgtcgc aaaattccgg
     2101 cccgtctgtt tatgtgatgc acgggagtta caccgccgcg caacatttta gcggcggttc
     2161 gtcttcgcct tatacggttt attccagttc aactcctgca cccgctcaca gcgggcacaa
     2221 cgaaggaaac aattcctacg caattcctgg cagtttcaca gcttccgaac atttgaacgc
     2281 ctcctcaacg caagcccgcg gaacggattt ttccagcccg cggcattctc atgaacagca
     2341 accggcgcaa gccgagtcgc acccgcattt tgaatcacac gctgaatcgc acaccgaatc
     2401 tcatgccgca acgtattcgg ctcccgctcc ggcttcgcat tcgtcatctt cgtcatcgag
     2461 ctcgtcggga agttcttctt cgggcagaag gtgagaaaaa accgcctgga aattttacag
     2521 gacaaactcg acgtcgcact tgtcgcgcaa acgccgttct tcggcatcgg gcagggagtt
     2581 gaagaaatcc aatcgtgatt cgcaaagctc aatcatcgct tccaatccga cacgcacata
     2641 acgcgattcg aagccgcttc catcaggcaa ctcaagtgta tgtcggaggg tgattagatg
     2701 atcgttactc aagctatcac ctcgatgaga cagcgatgat ttgttgatgg atgcgttcag
     2761 tgccataccg tctgcaaagt tcgatgaatt tttcaccgtt gatatcgata tgattgatct
     2821 ctgccaacca taccacatca agcaagtctt tcaattcccg tttgccatgg gtgtatttca
     2881 gggcgtggag tttcaatgca agaatatgct ccaaagccgg gaccctgaat tccatccctt
     2941 tcattttcac ttcaatggat tcgggataca ttttggaaaa tgtttgctga tttacgaaca
     3001 tcaaatccac aggccaagtt gtgccttcgg aagcggtgaa ttgcataaat gtgccctgtt
     3061 catgatgctc gctgtaaccc agcgaggcca ttaaactctt ccatgcttcc cgttcttcat
     3121 tccgcaccaa aaggtcaata tcggaagtca ctcgttcata cccatgtgca ttaattgcaa
     3181 gcccgccaat cacgaggaac tgcagtccga gttcactgcc ccgccgattt aatgccgtca
     3241 aaatatccat ggctgtccca agctggttct aaactttata ctggctttgc cgaaatacga
     3301 ggcaaaatgt gcgggactaa ggctttaccg ccgcggcctt gagcatttcc gccaccaact
     3361 cgtcgctggt ccatttattg tctggaaaga ttttttggat tcgcccgcgc gtgtccacca
     3421 cgacagtgcg caggttgtgc gtcaccgccc cactttcatc gtggccgaaa tattcgccca
     3481 cctggtcgcc gatggccgtg atgtccacca aatcgcccgt gagaaaactc caatgctcag
     3541 gctggtaatc ataacgcccg gcatacgcct tgagttgcgc gggcgaatca tattcggtgt
     3601 cgaacgaaat cgaaaacaaa tgccagttgg tcggcgcgtt gggcatggcg agcaatttct
     3661 tttctgccgc ctcgaaattg cccgacatca gcgggcaaaa tgtcggatac gggcagcgcg
     3721 tgaaaaaaaa tgtgaaagca aaagcctgcc ccttgaattg ttttgtgctg accgcctgtc
     3781 ccagttcgtt ggtgaaatgg tactccggca gcacatctcc cacttgcaac gggtccacgt
     3841 cgcgcaccag ccggaacgtc ggacgcgacg gcaactcggt cgcgcgcgca gcggtcaatt
     3901 tgatcacgtg ctcgatccac gcgtcattct ccgtgacaac catttggaag ctgatcaaat
     3961 cgccggatcg caagccgcgc aattcattcg tgttccgcac ctcgaacggc atcgtcatcg
     4021 ccggcatgta atgcggaact tcctcatgct tgatgacgac agttttgccg tcggatttca
     4081 attccttgac caatcctttg acctgaaaaa tttgctggtt cgtgtgcgcg gacggagttg
     4141 cggcggtcgt ctgttccttg caggaaagca gaacgaacgg ccccattgcc acaatcatca
     4201 aaaataaaat ccgccgccaa aacatcgtcg tgaaactttc ccataaacaa ctgttttggc
     4261 aagggtctgg agaaagttcc cgcgcggctt gccagagccg tgcgttttgt ggtttatttc
     4321 cttcgaatgc caacgtcatc ctcaccgagc cagagcgcgg aagattatct cgaacgcatc
     4381 cacgagttga tcgaggaaaa gggctatgcg cgcgtggtgg acatcgcctc gtcgctgaag
     4441 gtgaagcagg cgtccgtgac cagcatggtg cagaaacttg gcgaggcggg ttatctcaaa
     4501 tacgaaaaat accgcgggct catcctgacg aacaagggcc gcgaagttgc gcaaaacatt
     4561 cacgaccgtc acgagacgct ctctcgattt ttttcactgc ttgggttgga cgccgaaacg
     4621 caacggcgcg acattgaagg catcgaacat catctcagcc cggcaacggt cgaggtgctc
     4681 gccgacctgg ccggattttt cgaggaacat ccggagacgt tgcggcaatt tcagcagtcg
     4741 cgtcgcgctt ccaagcggaa gggtgcgtaa gcgttcgtta cgctttttgt ttgcacatcg
     4801 cttccatgcg cttgcgcatc tcctcgggcg ccacatcttc cttgtgcgtt gcgatgtgcc
     4861 aggtatggcc gtaaggatcg gtgagcgtgc ccatgcggtc gccgtaaaac ttgtcctcca
     4921 caggacggat cagttttgcg cccgcagcca cggcctgttt caccatcgcg tccacatctt
     4981 gaacgtagag caggatggaa atgggagaac cgccaagtgt ttgcgggctt ttcgcgtcca
     5041 tctctttcga ctcgtccgcc agcatgaaag gcgaatcacc gattttcatt tcggcgtggc
     5101 cgatcctgcc atccggcccc ggcatgcgca tgcattcggt cgcgccaaaa gcctttttgt
     5161 aaaactccat gccttccgcc gcgcccttga gaaccagata aggtgtgatg gagtgataac
     5221 catcgggaac aggtttggtt gccataagta tgttgcttac caatcgctca tcaagggcgg
     5281 cttggcaacc gttcatgtca aaaatttacc tacggaaaca aaccgcggat tttcttggcg
     5341 tcgcggacgc ggctgatgcc aaggctcaat gcggcctggc gcatcggcat cttttgcttg
     5401 cgactcaacg cgagtgtctg cgtgaatgcg ccttccaaga tgcggaacag cttgtctgtg
     5461 acctcggttt cgttccagaa aaagctctgc aaatcctgca cccattcgaa ataggaaacc
     5521 accacgccac ctgcgttgca caacacgtcg ggaatgagaa agatttggtc gcgctgttcg
     5581 atgatggcgt cggcttcggg attggtcggg ccgttggcgg cctcggcaag gattcggcac
     5641 tcgattttgc cggcgttctg tttcgtgatt tgccgttcca acgcggcagg aaccaaaata
     5701 tcgcaaggcg tgacaagcaa ttgctcgttg gaaatcggct cggcttcggg aaagccgaca
     5761 acagttttat tttcgttggc gaattggtcg agtttccaga gatcaagtcc cttggcgttg
     5821 aacaccccgc cgaaagc
//
LOCUS       DASZMM010000419         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3478232, whole genome shotgun sequence.
ACCESSION   DASZMM010000419 DASZMM010000000
VERSION     DASZMM010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3478232"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /gene="polX"
                     /locus_tag="VG754_10080"
     CDS             complement(<1..1570)
                     /gene="polX"
                     /locus_tag="VG754_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HWF19609.1"
                     /translation="MEKDQVAEVLVEIGILLELKGENPFKTRAYTNAARALEALREPL
                     TDLITQERLGEIKGIGDALQKKITELVTTGKLPYYDDLKASMPPGLLEMLQIPGVGPK
                     KVKVLYEKLGIKSVEELEAACKSGKVAELDGFGEKTAAKICEGINFRRNYASRHLLVE
                     ALTVSEPILESLRQHPDVIRCSTAGSLRRFKEIVGDIDFLVSSKKPAEIIEAFTTEPG
                     VISVSAKGETKATVILKGGIQSDLRVVSDAEFPFALAYFTGSKEHNIIMRQRAIQRGL
                     RLNEYGLFKSKEETRDPKLLVPCKTEEEIFEQLGLAYIPPELREDQGEFDAAEKHEIP
                     RLLEWTELRGSLHNHTNWSDGRDTLEEVADYMRELGCQYWAITDHSKSSVQAHGLEPG
                     RLRNQLLEIKKLNQRFADDGIEFRLLAGAEVDILKDRVDYDDDLLAQLDVVVASLHAG
                     FSQNEADNTKRLIRAAENPFVHIMGHLTGRLLLEREGYKVNQQAVIDACAATGTWIEL
                     NANPYRFDMDWRMWP"
     gene            1894..1990
                     /gene="ffs"
                     /locus_tag="VG754_10085"
     ncRNA           1894..1990
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VG754_10085"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          490 a          653 c          628 g          531 t
ORIGIN      
        1 aaggccacat gcgccaatcc atgtcgaaac gatacggatt cgcgttcaat tcaatccacg
       61 tgccggtcgc ggcgcacgca tcgatgaccg cctgctggtt caccttgtaa ccctcgcgtt
      121 ccagcagcaa tcgcccggtc aggtggccca tgatatgaac gaaaggattt tcagccgcgc
      181 ggatgagccg cttggtgttg tcggcttcgt tttgggaaaa accggcgtga agactggcaa
      241 ccaccacgtc gagttgcgcg agcaaatcgt catcgtaatc aacgcggtcc ttgaggatgt
      301 ccacttcggc gccagccagc agccggaatt cgatgccatc atcggcaaag cgctggttca
      361 gctttttgat ttccaggagc tggttgcgca gacgccccgg ttcaaggccg tgcgcctgaa
      421 ccgacgattt ggaatggtcg gtgatggccc aatattggca accgagttcg cgcatgtaat
      481 cggcaacttc ctccagcgtg tcccggccat cgctccagtt ggtgtgattg tggagcgaac
      541 cgcgcaactc ggtccattcg agcaaacggg gaatctcgtg tttctccgcg gcgtcgaatt
      601 cgccctggtc ttcgcgcaat tcgggcggga tgtaagcgag gccaagctgc tcgaaaattt
      661 cctcctcggt cttgcacggc acgagcaatt tcggatcgcg cgtttcctcc ttcgatttga
      721 acaagccata ctcattcaaa cgcagaccgc gttggattgc gcgctggcgc atgatgatgt
      781 tgtgctcctt gctgccggtg aaataagcga gcgcgaacgg aaattcagcg tcgctcacca
      841 cgcgcaggtc ggattggatg ccgcccttca gaatcacagt ggccttggtt tcgcccttgg
      901 cgctgacgct gatgacgccc ggctccgtgg tgaatgcttc aatgatttcc gccggtttct
      961 tcgatgaaac aagaaaatca atgtcgccga cgatttcctt gaaacggcgc aaactgcctg
     1021 ccgtgctgca acgaatcaca tcaggatgct ggcgcaaact ttccagtatc ggctcggaaa
     1081 cggtcaatgc ctcgaccaac agatggcgcg aggcgtaatt gcgccgaaaa ttgattcctt
     1141 cacagatttt ggcagcggtc ttttcgccaa aaccgtccag ttccgcgacc ttgccggatt
     1201 tgcacgccgc ttccaattcc tcgacggatt tgatccccag cttttcatac aaaactttca
     1261 ccttcttggg gccgacgccg gggatttgca gcatctccaa gagaccgggc ggcatcgatg
     1321 ccttcaaatc atcgtaatat ggcagcttgc cggtcgtgac gagttcggtg atttttttct
     1381 gcaatgcgtc gccgatgccc ttgatttcgc cgaggcgttc ctgcgtgatg aggtcggtga
     1441 gcggttcgcg caatgcttcc aaggcgcgcg cggcattggt gtaggcgcgg gtcttgaacg
     1501 gattttctcc cttcaattcg agcaaaatcc cgatctcgac aagaacttcc gccacctgat
     1561 ctttttccat gaaaataata tgcggcgcgc tccgcgaagt ttaaagttta atgtgcgagc
     1621 gggtgcatcc acgaattgtc ccggagcgcg gctcggtgag ccgcagcagc gcgcgacaaa
     1681 cgggacgtgc caaactaatt catcgcacct atccttccca cattgctgcg gctcaccgag
     1741 ccgcgctccg ttgtgcagcc gtcaatagct tcacccgctt cgcccgcaca tccggccagg
     1801 caaacaaatc ggggcaatgc caagatgcgc ccgggaacgg cacgcgtcgg gtttctgctt
     1861 gaagaaggcg gggggctgct gcagattggg tgttgccagg gcccatggaa tgtggactta
     1921 cgaaccccgc cagggcagga atgcagcaac ggcagtttgc tctgcatccg ccgtggtcac
     1981 cctggcattt taatttacga tttgggattt acgatttacg agttggaaaa gctggccagc
     2041 gcgtgaaatt attttccttt ctgggttgag attacctctt ttcccccgac cctcttccgc
     2101 aagggggcga gggagaagaa agccaccggc agtcacgtga caatatccga aatttaatat
     2161 cgcaaaacat cgcgtaaatc gtatatcgta aatcgcaaat cgcatgtctt atcaagtcat
     2221 agccagaaaa taccggccgc agcgtttctc ggatgtcgtg gggcaagaac atgtcacaca
     2281 gactttgtcc aatgccatcg cg
//
LOCUS       DASZMM010000420         5651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2091090, whole genome shotgun sequence.
ACCESSION   DASZMM010000420 DASZMM010000000
VERSION     DASZMM010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091090"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..2041)
                     /locus_tag="VG754_10090"
     CDS             complement(149..2041)
                     /locus_tag="VG754_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HWF19610.1"
                     /translation="MERKTQKLGLINLVMLVLVGAAAFALAKYSNTLAGLTATIFLGL
                     GVLVAAISWFQMGLEEREKLEKMEFEELTRSASSSALFNTGETEVFPAQRSREQFERF
                     FVPGFTVLLCLLQIGGAVLLWRWLQKIITVPLNEPAVPAAFFAVFFLLLFLPGKYSTG
                     IARMEKLRLLRPGASYLLLNAYLSALVVLAIVLVWLGFPEYDLYAARVLAGLLLLVGI
                     ESLISLIFELYRPRVKGKVERPVYESRLVSLLGQPEGLYYTAAQTLDYQFGFKVSETW
                     AFQLLKQWFPRLILIQLAVMLFSTCFVFIDAGQEGLLEHNGTPAGVLKPGAHFTWPWP
                     IDRVYRYATEQIQTINIGFVPDDSPMAGNTVLWTTPHNKEENFLVANREMPALENQTN
                     DATAGNRAPPVSLLTVSIPVQFQITNLTAWVYNNEDPVGLLTHISTREVVRYLVSVDL
                     EEIMSRSRWDAAQALRTRIQAAADIHKLGANIVFVGLQDIHPPGKVAPDYEKVVSAIQ
                     TKEATILSARAEAIKAINIADAQAFTTVTDARADSTRRRVDAMAQAALFTNQLPAYLA
                     APAVYAQRAYLETFTRSITNARTYVMLATNTQDVIILDLEDKIRKDILESATLPPPKN
                     APKPNP"
     gene            complement(2070..3032)
                     /locus_tag="VG754_10095"
     CDS             complement(2070..3032)
                     /locus_tag="VG754_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWF19611.1"
                     /translation="MRQFVTIATNAFMELVRQPVFLLLMTSSAVFEIFLATPFYFAFG
                     DEPKLVKNSVLAVMLLAGLFGAVLSASASLAREIRSGTALAVLSKPIGRTQFLLAKFV
                     GLALALTVVTYVNMLVALLASRMAFDAYGSTDLVALSIFAVCLVLGYLMGGFSNFFLR
                     RPFVSDAVFSVVLMVTVSFVIISFFSNKGEVQEFAKGVDWRLIPASMLILFALWVLAG
                     LALACSTRLEMIPTLAICSGLFLLGLMSQYLFSERAHAGSWWASVLYTVTPNWQLFWL
                     ADGLDTGAKDYYWGYVGKALGYAICYVGAALAAALMLFEERELA"
     gene            3378..3980
                     /locus_tag="VG754_10100"
     CDS             3378..3980
                     /locus_tag="VG754_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19612.1"
                     /translation="MKSISLLVVLSLLASAHLAKAVTITFDDLPTGNSALDWVVIPNG
                     YYGYNWTGFGAINATTVTPPSGYEQGMVSPKNVAFDDNDVTRQASISSASAFNLTSAY
                     LTAQIYNGMQVDVAGFIGATKIYDNIYIINSNAPTLINFDYQDVTKVTFTPENTSEIF
                     VMDNLTLSPVPESNRYAVFIAGAGLIAFALWRKKFNTSLE"
     gene            4163..5551
                     /locus_tag="VG754_10105"
     CDS             4163..5551
                     /locus_tag="VG754_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF19613.1"
                     /translation="MKRTAVLNVVGLTESLLGPATPRINRFLAQGAKSAVVPAFPAVT
                     STAQSTYLTGTTPAQHGIVGNGWYNRDLAEVQFWKQSNHLVHGRKIWEELRELEPDFT
                     CAKLFWWHNMYSSADYSITPRPMYPADGRKFFDIYSWPYSIRTEIKKDLGEFPFPTFW
                     GPAAGVKTPQGAPDAATRWIAESAKWIEKKYLPSLNLVYLPHLDYNLQRLGPKHSDVA
                     RDLREIDAIVGELIDFFTKHSVQVILLSEYGITQVDTPIHINRILRQYNWLAIKEELG
                     REMLDCGASAAFAVADHQIAHIYLNNPDIAEQVRSALPDGVQVLDRKEQASLGIDHPR
                     AGDLLAIAPENAWFTYYYWLDDNVAPDFARCVDIHRKPGYDPAELFLDPKIPAVKLKI
                     LRRLLQKKLGFRMLMDVIPLDATLVKGSHGCRPSNPGDQPLLITSRPELLPSKQIEAT
                     DVYHIIKRHVLY"
BASE COUNT         1312 a         1647 c         1515 g         1177 t
ORIGIN      
        1 gtcaagggtg cgggtcggct tgccgaacgt ggtgatgacc accacggtgg acttgcgcac
       61 ctgcacacag gaaaggatgg caaggcaaat caccgcgagc aacacgccgg tgcctagtgt
      121 gaaataattt cgtttcataa cttgaaaatc atgggttcgg ttttggtgcg ttcttgggcg
      181 gtggaagggt cgctgattcg aggatgtcct tccgtatctt gtcttcgagg tcgaggatga
      241 tcacgtcctg ggtgttcgta gcgagcatca cataagtgcg ggcgttggtg atggagcgcg
      301 tgaaagtttc cagataagcg cgctgcgcat aaaccgcggg cgcggcgaga taagccggca
      361 attggttggt gaacaatgcc gcctgcgcca tcgcgtccac gcgccgccgc gtgctgtccg
      421 cccgcgcgtc cgtcacggtc gtgaaagcct gcgcgtcagc gatgttgatc gccttgatgg
      481 cttcggcccg cgcggaaagg atggtcgctt ccttggtctg gatcgctgaa acaacttttt
      541 cgtaatccgg cgcgactttc cccggcggat ggatgtcctg caagccgacg aacacaatgt
      601 tggccccaag tttgtggatg tccgccgcgg cctggatgcg cgtgcgcaag gcctgcgccg
      661 cgtcccaacg cgagcgggac atgatctcct ccaaatcgac gctcacgaga taacgcacca
      721 cttcgcgcgt ggagatgtgc gtgagcaggc cgaccgggtc ttcgttgtta tacacccacg
      781 ccgtgagatt ggtgatctgg aattgcaccg ggatgctgac ggtcaacaaa ctgaccggcg
      841 gcgcgcggtt gccagccgtg gcgtcgttgg tctggttttc caacgcgggc atttcgcggt
      901 tggcgacgag gaaattttcc tctttgttgt gcggcgtggt ccagagcacg gtgttgccgg
      961 ccatcgggga atcgtccggc acgaaaccga tgttgatggt ctggatctgt tcggtggcat
     1021 agcggtaaac gcggtcaatc ggccacggcc atgtgaaatg cgcgccgggt ttgagcacgc
     1081 cagcgggagt tccgttgtgt tcgagcaaac cttcctgccc ggcgtcaatg aaaacgaagc
     1141 aggttgaaaa aagcatcacc gcgagttgaa tcaggatgag ccggggaaac cattgcttga
     1201 gcaactggaa ggcccatgtc tcagacactt tgaaaccgaa ctgataatcc agtgtctgcg
     1261 ccgcggtata atacaacccc tccggctggc cgagcaggct tacaagacgg ctttcataca
     1321 cgggccgttc gaccttgcct ttcacgcggg gacggtaaag ttcgaagatc aaactgatga
     1381 gcgactcgat gccgacgagc agcaacaatc cagccagcac gcgggccgca tacaggtcgt
     1441 attccggaaa accgagccag acaagcacga tggccaggac gaccaacgcg ctgagatagg
     1501 cattgagcag cagataactt gcgccaggcc gcagcaggcg gagcttttcc atccgcgcaa
     1561 tgccggtcga atatttgccg ggcaaaaaga gcagcagaaa gaacaccgca aaaaatgccg
     1621 ccggcacagc cggttcgttg agcgggacgg tgatgatttt ttgcagccag cgccagagca
     1681 ataccgcgcc gccgatctgg agcaggcaca gcagcaccgt gaaacccggc acgaaaaagc
     1741 gttcgaactg ctcgcgcgaa cgttgcgcgg ggaaaacttc tgtttcgccg gtgttgaaca
     1801 gcgcggaact ggaagcggac cgggtcagct cctcaaattc cattttttcc aacttttcgc
     1861 gttcttcgag gcccatctgg aaccagctta tcgcggcgac cagcacgcca aggccgagga
     1921 agatcgtcgc ggtcagcccg gccagcgtgt tgctgtattt ggccagcgca aacgccgccg
     1981 cgcccaccaa caccagcatc accaggttga tcagcccaag tttttgtgtt tttcgttcca
     2041 tgctcagact ttgcttatca atgactcgac taagccaatt cgcgttcttc gaacaacatc
     2101 aatgccgcgg ccagcgccgc gccgacataa caaatcgcat aacccagcgc cttgcccacg
     2161 taaccccagt aatagtcctt cgccccggtg tccaagccgt cggcgagcca gaaaagctgc
     2221 caatttggcg tcaccgtgta aagcaccgaa gcccaccacg aacccgcgtg cgcccgttcg
     2281 ctgaacagat attgcgacat caatcccagc aaaaataacc cggagcatat cgccagcgtg
     2341 gggatcattt caagccgcgt ggaacaggcc agcgccagcc ccgccaacac ccacaaggcg
     2401 aacaaaatca acatcgatgc cggaatcagc cgccagtcca cgcctttcgc gaattcctgc
     2461 acttcgccct tgttgctgaa aaaactgatg atcacgaatg aaaccgtcac catcaggacc
     2521 acgctgaaca cggcgtccga gacaaacgga cgccgcagaa aaaaattgct gaacccgccc
     2581 atcaaatatc cgagcaccag gcagaccgca aaaattgaca gcgcgaccag gtcggtcgag
     2641 ccgtaggcgt cgaacgccat gcggctcgcc agcaacgcca ccagcatgtt cacataagtg
     2701 acgaccgtca gcgccagcgc aaggccgacg aatttcgcca gcaaaaattg cgtgcggcca
     2761 atcggcttgg acaacaccgc cagcgccgtg cccgaccgga tttcgcgcgc gagcgatgcc
     2821 gaagcgctca acaccgcgcc gaacaatcct gccagcagca tcaccgccag cacgctgttt
     2881 ttcaccagct tcggctcgtc gccaaaggcg aagtaaaacg gcgtcgccaa aaaaatttca
     2941 aacacggcgg agctggtcat gagcagcagg aataccggct gccgtaccaa ttccatgaac
     3001 gcgttcgttg cgatggttac aaattgtcgc attttgctga aaccaaggct ggctagccta
     3061 tcaaccgggc gaacgattgc aaatgcttat tccacgactt tggtgtcatg tcgcgagaat
     3121 aacgcaaagt aacccgttga aaactgtgcg tttgcaggca cagccctgct ggatgctcgt
     3181 aagtgaggcg gtcatgctct ttggatgcat caagcgcgtt aatgttcaaa atggactcgc
     3241 cgcctcaagc gctcgggatt tcattatgtg cattgaaacg cacacttctc aatcgcctgg
     3301 catgtgttat cattccgcac gcactgagaa attaaatccg accatcaatc aattctaatc
     3361 acgattgccc aaaaactatg aaaagcatct cccttctggt cgttctttct ttgttggcat
     3421 cggcccacct cgcaaaagct gtcacgatca ctttcgacga cctgccgacg ggcaatagtg
     3481 cgttggattg ggtggtgatt ccaaatgggt attatggcta taattggaca ggttttggcg
     3541 ccatcaatgc cacgactgtg acgcctcctt ccggttacga acagggaatg gtttcgccga
     3601 agaacgtggc ctttgacgac aacgatgtca cccggcaggc gtccatcagc agtgcatcgg
     3661 catttaattt aacttccgcc tatttgacgg cgcagattta taatgggatg caagttgacg
     3721 tcgcaggctt tatcggcgca accaagattt atgacaacat atacatcatc aactccaatg
     3781 cccccacgtt gatcaacttt gattatcagg acgtcaccaa agtgaccttc accccggaga
     3841 acacttcgga gatattcgtc atggacaacc tgaccttgtc accggtcccg gaatcgaacc
     3901 gttacgcggt attcatcgca ggcgccgggc tgattgcatt tgccctgtgg cgaaaaaaat
     3961 tcaacacatc tcttgaatag tcgcgcggcc gtgcgcgaaa cgccagccgc ggcacatggc
     4021 cgaaatcctg agtgttccaa gctgctgcgg ctggtcctgc ggacacagcc gcgctccgtc
     4081 cggaccggtg ctccaacggg atagcgtcac agccacaaaa tccttgcttc attgcggttt
     4141 agcattccgc taagctgcct gcatgaaacg cacggcggta ctcaatgtgg tcggccttac
     4201 cgaatcgctt ctcggtccgg ccacgccgcg catcaaccgc ttccttgctc aaggcgcgaa
     4261 atccgccgtc gttccggcat ttcccgcagt cacctccacc gcgcaatcca cgtatctgac
     4321 cggcacaacg cccgcgcaac acggcatcgt cggcaacggc tggtacaatc gcgacctggc
     4381 cgaggtccag ttttggaaac aatccaacca tctcgtccat ggccgaaaaa tctgggagga
     4441 attgcgcgag ctggaacccg acttcacctg cgcgaaactt ttctggtggc acaatatgta
     4501 ttcgagcgcg gattattcca tcactccgcg cccgatgtat cccgccgatg gacgaaaatt
     4561 ttttgacatc tattcgtggc cttattccat ccgcaccgag attaaaaagg atttgggcga
     4621 attcccgttc ccaacatttt ggggcccggc cgcaggcgtc aaaactccgc aaggcgcgcc
     4681 ggacgccgcc acgcgctgga tcgccgaatc ggcaaagtgg attgaaaaaa aatatttgcc
     4741 gtcgttgaac ctggtctatc tgcctcatct cgattacaac ctgcaacgtc tcggtccgaa
     4801 gcattccgac gttgcgcgcg acctgcgcga gattgatgcg atcgtcggcg aattgattga
     4861 tttttttacg aagcattcgg tgcaggtgat tttgctttcc gagtatggca tcacgcaggt
     4921 ggacacgccc atccacatca accggatatt gcgccaatac aattggctgg ccatcaagga
     4981 ggaactgggc cgcgagatgc tcgattgcgg ggcgagcgcg gcctttgccg ttgccgacca
     5041 tcaaatcgcg catatttatc tcaacaatcc ggacattgcc gaacaggtcc gctccgcatt
     5101 gccggatggc gtgcaagttt tggatcgaaa ggagcaggca agtttgggca ttgaccatcc
     5161 gcgcgcgggc gacctcctcg ccatcgcccc ggagaacgca tggttcactt attactattg
     5221 gctggatgac aacgtcgcgc cggactttgc gcgttgcgtg gacatccatc gcaagcccgg
     5281 atacgatccc gcggaacttt ttctcgatcc gaaaattccc gcggtaaaat taaaaatcct
     5341 tcggcgtctt ctgcaaaaaa agctcggctt ccgcatgttg atggatgtga ttccgctcga
     5401 tgccacgctg gtcaaaggct cgcatggatg tcgtccgtca aatcccggtg atcaaccgct
     5461 cctcatcacc agccgcccgg aattgctgcc gtccaaacaa attgaggcaa ccgatgtcta
     5521 tcacatcatt aaacggcatg tgctgtattg actgaaaatt tcactaaaca gggatgaaca
     5581 tcgcattttt gcattcgtaa ggtttcgctg aaatgacccg gaacaccggg ggcgcaactc
     5641 actattttgc a
//
LOCUS       DASZMM010000421         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2643633, whole genome shotgun sequence.
ACCESSION   DASZMM010000421 DASZMM010000000
VERSION     DASZMM010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643633"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2443
                     /locus_tag="VG754_10110"
     CDS             <1..2443
                     /locus_tag="VG754_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF19614.1"
                     /translation="LEEQEFLRAKREAEDRAAYYADLFKAAPVGFLVLNFSGAILDIN
                     EAAANLLRRKPQELKGRLFTLFLPEEQIAGFFSHLRRCTKTAKPQVTELAVQTSEKDL
                     VPVELVSTSTVGPDQITYYQTVMTDITAHKLAEQAGLDSRRNYRNLVNSIEGIVWEGT
                     DKPGEFTFVSRQAQQMLGYSPERWLRVPDFWENRLHSEDRARVLNVRRQAIAEKRSYL
                     VEFRIYDSCRRVVWMRDSGNVVRDENGRFHLQGVMMNITDLKEAEEALREETRMLETI
                     NRIGTLLTGQLDLTKLVAAIAEAGKQVTGAKFGAFFYKRHEGVNDESSVYITTGTTRD
                     IFDHLPLPHHRPGARPMEAESEAIRIDDLLDDKCAPKPALRGSSSETPPENVRSYLAM
                     PVVSRSNEVLGGLLYAHPMPGVFTERAQHLLAGIASEAGIALDNARLHRAVSKSESHF
                     RQLADAMPQIVWTARGDGQVDYFNSRWYEYTGLSNGSLKEGGWLPLLHAEDRERCAAA
                     WKKSLAAGAPFEVECRLQDRRTGRYRWHLIRAVPNHDEQYRVVGWFGTSTDIEDQKRV
                     EGEIRELNTALERRVAERTAQLQASNQELEAFSYSVSHDLRAPLRSIDAFSQLVREDY
                     EDKLDEQGKEYLGIVGNASRQMGQLIDDLLHLSRVTRSEMRRGTVDLSALATTIMDNL
                     KQLEPRRKVEMVIAPGLEAQGDGQLLRVVLENLLNNAWKFTSRKPEARIELGVEGQNA
                     ERVFFVKDNGAGFDMTYSNKLFAAFQRLHSTSEFPGHGIGLATVQRIIHRHGGRIWAN
                     SAVDQGATFYFTLPQ"
     gene            2511..>3569
                     /locus_tag="VG754_10115"
     CDS             2511..>3569
                     /locus_tag="VG754_10115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF19615.1"
                     /translation="MKTTNTVRLRLRVTAAAETILRGGHPWLYAESVREQNREGKTGE
                     LAVIYDRKDEFLAVGLFDADSPIRVRVLHAGKPQTLDHAWWLRRLQEAVKKRLMTFDG
                     TIPNSELGTLNSEPAKPTTGYRSINGESDGCPGLVLDRYDKTLVLKLYTAAWLPRLDE
                     IVELIADNVPSECVVLRLSRNIQEKAAEFQQHDGQILRGEALSGPVIFLETGLRFEAD
                     VVRGQKTGFFLDQRENRRLVESLAKGRTMLNAFSFSGGFSLYAARGGAKSVTDLDISA
                     HALAGAKRNFALNEEIPEVARCPHETIQADTFEWLTETRRRFDLVVLDPPSLAKRESE
                     RAGAIHAYSRLAALGIRAL"
BASE COUNT          791 a          938 c         1120 g          720 t
ORIGIN      
        1 attggaggag caggagtttt tgcgcgcgaa gcgcgaggcg gaggaccgtg ccgcatatta
       61 tgccgacctt tttaaggccg ccccagtcgg ttttctcgtg ctcaattttt caggagcgat
      121 tctcgacatc aatgaagcgg cggcgaacct tctgcgcaga aaaccgcaag aactcaaggg
      181 caggcttttc acacttttcc tgccggaaga acagatcgcc ggatttttca gccatctccg
      241 tcgctgcacg aagacggcaa aaccgcaggt gacggagctt gcggtccaga catcggaaaa
      301 agaccttgtg ccggtggaac tggtcagcac atccaccgtc ggtccggacc agattacgta
      361 ttatcagacg gtgatgacgg acatcacggc gcacaagctc gcggaacagg ctgggctcga
      421 cagcaggcga aattaccgca acctggtcaa ttccatcgag ggcatcgtat gggaggggac
      481 agacaagccg ggcgagttca cttttgtgag caggcaggcg cagcagatgc ttgggtattc
      541 gccggagcgc tggctgcgcg tgccggattt ttgggaaaat cggctgcact ctgaagaccg
      601 cgcgcgcgtt ctcaatgtcc gccgccaggc catcgcggaa aaacggagtt acctggtgga
      661 atttcgcata tacgattcct gccgccgcgt ggtctggatg cgcgacagcg ggaatgtggt
      721 tcgggatgaa aacggccgct tccatctgca aggggtgatg atgaacatca ccgacctcaa
      781 ggaggcggag gaagcgttgc gcgaagaaac gcggatgctt gaaaccatca atcgcatcgg
      841 caccttgctc accggacagc ttgatctgac gaaactcgtc gcggccatcg cggaagccgg
      901 caagcaggtg acgggcgcga aattcggcgc gttcttttac aaacgccatg agggcgtgaa
      961 cgatgaatcg tctgtttaca tcacaaccgg gacaacgcgc gatatttttg atcatttgcc
     1021 gttgccgcac catcgtccgg gcgcgcggcc catggaggcg gaaagcgagg ccatccgcat
     1081 tgacgatttg ctggatgaca aatgcgcgcc caaaccggcc ttgcggggtt cttcttcgga
     1141 gacgccgccg gaaaatgtcc gcagttatct cgccatgccg gtggtttccc gctcgaacga
     1201 agtgctgggc ggattgttgt acgcgcaccc gatgccgggg gttttcaccg agcgggcgca
     1261 acatctgctg gcgggcatcg cgtcggaagc tggcatcgcg ctggacaacg cccgattgca
     1321 ccgcgccgtg agcaaaagcg agtcccattt tcgccagctt gcggacgcca tgccgcaaat
     1381 cgtctggacc gcgcgcgggg atgggcaggt ggattatttc aacagccgct ggtatgaata
     1441 taccggcctg tccaacggct cattgaagga aggcggctgg ctgccattgc ttcatgcgga
     1501 agacagggaa cgatgcgctg cggcatggaa aaaatcgctg gctgcgggcg cgccgttcga
     1561 ggtggagtgc cggcttcaag accgccgtac gggaagatat cgctggcatt tgatccgcgc
     1621 cgttcccaat cacgacgaac agtaccgggt cgtcggctgg ttcggcacgt ccacggatat
     1681 cgaggaccaa aaacgcgtgg agggagagat ccgggaattg aacactgcct tggaacggcg
     1741 cgtcgcggaa cgcaccgcgc agttgcaggc gtccaaccag gaactggagg cgttcagcta
     1801 ttcggtctcg cacgacttgc gcgccccgtt gcggagcatt gacgcgttca gccagttggt
     1861 gcgggaagat tacgaggaca agctggatga gcaggggaag gaatatctgg gcatcgtcgg
     1921 aaacgcgagc cggcagatgg gacaattgat cgacgatctg ttgcacttgt cgcgcgtgac
     1981 gcgcagtgaa atgcgccgcg gaacggttga cctgagcgca ttggcgacaa ccataatgga
     2041 taatctcaag cagctcgagc cgcgacgaaa ggtggagatg gtgatcgcgc cggggttgga
     2101 ggcgcagggt gacgggcagt tgctgcgcgt cgtcctcgaa aatctgctca ataacgcatg
     2161 gaaattcacc agccgcaagc cggaggcgcg catcgagttg ggcgttgaag ggcagaatgc
     2221 cgagcgggtg ttttttgtga aggacaacgg cgctggattc gacatgactt actcaaacaa
     2281 gttgttcgcg gcgttccaac ggcttcattc gaccagcgaa tttcccgggc acggcatcgg
     2341 tctggccacc gtgcaacgca tcatccatcg ccatggcgga cgcatctggg ccaattcggc
     2401 tgttgaccag ggcgcaactt tctattttac gctgcctcag taagtcaagg tttaaggttt
     2461 aaagtagatt gtcaccgtct attcttgagt cgttcgtgcc ctttaagtct ttgaaaacca
     2521 ccaacaccgt caggctgcgc ttgcgcgtga ctgccgctgc cgaaaccatc ttgcgcgggg
     2581 gacatccatg gctctatgcc gaaagtgtgc gcgaacaaaa tcgcgaagga aaaaccggtg
     2641 aactcgcggt gatctatgat cgcaaggacg aatttctggc cgtcggtttg ttcgatgccg
     2701 attcgcccat ccgcgtccgc gttttgcatg ccggcaagcc gcaaactttg gaccacgcat
     2761 ggtggttgcg gcggttgcaa gaagcggtga aaaaaagact gatgacattc gacggcacga
     2821 ttcccaactc ggaactcgga actttgaact cggaaccggc gaagcccaca acagggtatc
     2881 gtagcatcaa tggagaaagc gatggctgcc cgggattggt gttggatcgc tatgataaga
     2941 cgttggtgtt gaaattatac acagcggcat ggctcccgcg attggatgaa attgtggaat
     3001 tgatcgccga caacgtgccg agcgaatgtg tggtgctgcg tttgagccgg aacatccagg
     3061 aaaaagccgc cgaatttcag cagcacgacg gacagatttt gcgcggcgaa gctttgtccg
     3121 gtccggtgat tttcttggaa acgggattgc gtttcgaagc agacgttgtg cggggacaaa
     3181 agaccgggtt ctttctggat caacgcgaga accggcggct cgttgaatcg ctcgcgaaag
     3241 gacggacgat gttgaatgca ttcagtttct cgggcggatt ttcgctttac gcggcgcgtg
     3301 gtggcgcaaa atcggtgacg gatttggaca tcagcgcgca cgcgcttgcc ggcgcgaaac
     3361 gaaattttgc attgaacgaa gaaattccag aagtggcgcg ttgtccgcac gagacaattc
     3421 aagcggatac gttcgagtgg ctgactgaaa caaggcggcg tttcgatttg gtggtccttg
     3481 acccgccctc attggcaaag cgcgaatcag aacgcgcggg cgcaatccac gcctattcgc
     3541 ggctggcggc gctcggaatt cgcgcattg
//
LOCUS       DASZMM010000422         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4021629, whole genome shotgun sequence.
ACCESSION   DASZMM010000422 DASZMM010000000
VERSION     DASZMM010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4021629"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VG754_10120"
     CDS             complement(<1..451)
                     /locus_tag="VG754_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWF19616.1"
                     /translation="MLHLPQPARALKWFGWTDRGKVRPNNEDAFVGLQFDSREIHHLG
                     KFGNASMEKMDFAFAVSDGMGGALAGEYASRVTVDKIAALLPRSFRNSALGLESGFED
                     LLTEIFGEVHRALTYLGGSYEECFGMEATLSLCWFTPGWLYFGHIGDS"
     gene            complement(808..1191)
                     /locus_tag="VG754_10125"
     CDS             complement(808..1191)
                     /locus_tag="VG754_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HWF19617.1"
                     /translation="MLNVKDTLRSTVNLTFDGRVIKTYHGPDALTRFDREGKILRHLE
                     TRGCNFVPRLLEADREKIRIITTNCGSRVEHLDEARRRELFAELQQYGVLHDDPDMRN
                     VTYRQTDGRFCLVDFELASLIHGPH"
     gene            1526..>2482
                     /locus_tag="VG754_10130"
     CDS             1526..>2482
                     /locus_tag="VG754_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HWF19618.1"
                     /translation="MPKYKLEYIWLDGYQPVPNLRGKTTIKEFDSFPTLEQLPFWGFD
                     GSSTRQAEGHSSDCMLKPVAIYPDKTRVNGALVMCEVMMPDCKTPHPSNARATILDDP
                     GAWFGYEQEYFLYKDGAPLGFPAGGGFPPPQGEYYTGVGYKNVGDVARKIVEEHLELC
                     LYAGINHEGINAEVAKGQWEFQIFGKGSKTAADQMWMARYIMMRLCEQYCVDINWHCK
                     PLGKDVDWNGSGMHTNFSTTHMREVGGKKYFERLMNAFDKYKNEHIAVYGPDNHLRLT
                     GLHETQSIDKFNYGIANRGASVRVPHSFVNAGYKGYLEDRRPN"
BASE COUNT          567 a          714 c          665 g          536 t
ORIGIN      
        1 ggctgtcgcc gatgtgtccg aaataaagcc agcccggcgt gaaccagcag agactcagcg
       61 tcgcttccat cccgaagcat tcttcgtaac ttccgccgag ataggtgagc gcgcgatgga
      121 cttcgccaaa aatttccgtc agcaaatcct cgaaacccga ttccagtccc agcgcggaat
      181 tccggaacga gcgcggcagc agcgcggcga ttttatccac cgtgacgcgg ctggcgtatt
      241 cccccgccag agcaccgccc atgccgtcgc tgactgcaaa tgcgaagtcc attttttcca
      301 tcgaagcgtt gccgaatttc ccgagatgat ggatttcccg ggaatcaaac tgcagaccga
      361 cgaaagcatc ttcgttgttc gggcgcacct tgccgcggtc ggtccagccg aaccatttca
      421 acgctctcgc gggttgaggc aaatggagca ttttgtttgt tacattgtta cgacgttaaa
      481 tggtttcata agatgcaatt ggacacatcc gcgcactgcc ctggagcgcg ggtcggcgac
      541 ccgcagcaac gtgggaaaaa tgagtgtatg aattagtctg gcatggtcct gttcgtcgcg
      601 cgttgctgcg gctcatcgag ccgcgctccg tttcgcccga tgaacgcggc ggacaattgc
      661 ctggccagtc cgtaccggca tggccaacaa atcggggcag tgcgcggagg cgcccgatgc
      721 aattgcactt actacgcttc tgaccatgta acattgtaac catgtatcca atccaacttc
      781 atacgttgtt aaatttttcc gtcagtgtca gtgcgggccg tgaatcaacg acgccagttc
      841 aaagtcaacg agacaaaaac gcccgtccgt ctggcgatag gtgacgttgc gcatgtcagg
      901 gtcgtcgtgc agcacgccgt attgttgcaa ctcggcgaag agttcgcggc ggcgggcttc
      961 atcgaggtgt tcaacgcggc tgccgcaatt ggtggtgatg atgcggattt tctcgcggtc
     1021 tgcttccagc aggcgcggca cgaaattgca tccgcgcgtt tcaagatggc ggaggatttt
     1081 cccttcgcgg tcaaagcgcg tcagcgcatc cggcccatgg tacgttttga tgacacggcc
     1141 atcgaaggtg aggttcaccg tggaccggag cgtgtctttg acattcagca taacgcagtt
     1201 gctgagtatg gcgcatcggc aacgtcatga aaagccgctt tctggagggt cttaccatga
     1261 attgtagggt ttctcttatc cagagtggtc aaatttcgcc atggacacta aacaaaaaat
     1321 tataaaaaac gggctctttc gaaaatagcg cttcactcaa aacagcccgt catcacagga
     1381 taatttgagt ttccgcgcca attcgcctga aatcacttgc tgcttggcat tgtctctgct
     1441 gaaatcacgg aattcgatca agggccgggc atttggctcg gctttgtcgt cacgagtaat
     1501 tggccgttta acaacaatca cgcacatgcc gaaatacaaa ttagaatata tctggctgga
     1561 cggttatcag cccgtcccca acctccgcgg taaaaccaca atcaaggaat ttgattcttt
     1621 cccgaccttg gaacagctcc cgttctgggg ttttgacggc agctcgaccc gtcaggctga
     1681 aggtcacagc tccgactgca tgttgaagcc ggtcgcgatt tatcccgaca agacccgcgt
     1741 caacggcgcg ttggtgatgt gcgaagtcat gatgccggat tgcaagaccc cgcatccgag
     1801 caacgcccgc gcgaccatcc tcgatgaccc cggcgcatgg ttcggttacg agcaggaata
     1861 ttttctgtat aaggacggcg ctccgctcgg cttcccggcg ggcggcggtt tccctccgcc
     1921 ccaaggcgaa tattacaccg gcgtcggcta caagaacgtc ggcgacgtcg cccgcaagat
     1981 tgtcgaagaa catcttgaac tttgccttta cgctggcatc aaccacgaag gcatcaacgc
     2041 cgaagtggcc aagggccagt gggaattcca gatcttcggc aaaggttcca agaccgcagc
     2101 cgaccagatg tggatggccc gctacatcat gatgcgcctc tgcgaacaat attgcgtgga
     2161 catcaactgg cactgcaaac cgctcggcaa ggacgtggac tggaacggtt ccggcatgca
     2221 caccaacttc tcgaccaccc acatgcgcga agtcggcggc aagaagtatt tcgaacgcct
     2281 catgaacgcg ttcgacaaat acaagaacga acacattgcc gtttacggcc cggacaatca
     2341 tctgcgcctg accggcctgc acgaaacgca gtccatcgac aagttcaact acggcatcgc
     2401 caaccgcggc gcctcggtgc gcgttccgca cagcttcgtc aacgccggct acaaaggtta
     2461 tctcgaagat cgccgtccga at
//
LOCUS       DASZMM010000423         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3204550, whole genome shotgun sequence.
ACCESSION   DASZMM010000423 DASZMM010000000
VERSION     DASZMM010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3204550"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VG754_10135"
     CDS             complement(<1..1044)
                     /locus_tag="VG754_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19619.1"
                     /translation="MAELKKSPLTRRDFLRKTAMTTAGLWLGAHLSKAKSFSANNRLN
                     IGIIGTANRAEANISGVQKENIVALCDVDDHYLSAAAQRFPGAKTYNDFRKLLERNDL
                     DAVVISTADHTHAVATIAALQSGRHVYCEKPLAHTVSESRLVARTAGQYKNLATQMGT
                     QIHAGKNYRRVVELIRNGAIGNISECHVWCEKSWGGTGRVEGSPPVPSYLHWDLWLGP
                     MPERAYHSEYLPKNRRRWWDFGNGTLGDMGCHYMDLAYWALDLRHPLTIEAKGPPINP
                     ETTPSWLVVRYEYETHASKSPVQITWYDGGTQPELIKEGKILDWKNGVLFVGNKGMLL
                     ADYEHHRLLPESDF"
     gene            1237..1779
                     /gene="sufT"
                     /locus_tag="VG754_10140"
     CDS             1237..1779
                     /gene="sufT"
                     /locus_tag="VG754_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HWF19620.1"
                     /translation="MHNVTSVELKRDCEVVQIPSGNPILLPAGTPVDITQKLGGSYTI
                     HAQGGLFRVSPSDADALGFEAPAAAESSAQPGGPVDEKLVWDTLKTCYDPEIPVNIVD
                     LGLVYDMHIESLPSGNNLISVKMTLTAPGCGMGATIAGDAQQKLLYLPGVEEAVVEIV
                     WDPPWHQSMITEQGRRVLGL"
     gene            1829..1904
                     /locus_tag="VG754_10145"
     tRNA            1829..1904
                     /locus_tag="VG754_10145"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1862..1864,aa:Ala,seq:ggc)
     gene            complement(1929..2303)
                     /gene="acpS"
                     /locus_tag="VG754_10150"
     CDS             complement(1929..2303)
                     /gene="acpS"
                     /locus_tag="VG754_10150"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWF19621.1"
                     /translation="MLGIGIDIIEVARIQASHEKFGERFLNRILHPGEIAYCLSHKMP
                     APFLAARFAAKEAISKAFGTGIGAQLGWHDMEVARRESGEPYVILHGKGEELLKQRGG
                     RIVLISLSHTQQHATAVAVLEN"
     gene            complement(2303..3043)
                     /locus_tag="VG754_10155"
     CDS             complement(2303..3043)
                     /locus_tag="VG754_10155"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWF19622.1"
                     /translation="MLKLGVNIDHVATLREARYRGTGHGEPDPVAAALMAESAGAHGI
                     TAHLREDRRHIQDRDVWKLREVIKTRLNLEMANAPEIVVIALKLKPEIVCLVPERRQE
                     VTTEGGLDVAGNLEALKETRKKMNDAGIEVSFFIAPDASQVEASAKAGAQFIELHTGA
                     YAEHFDQKSARNIELERLIAAAKQAHGLGLKVNAGHGLNYQNLPVLHTVPHLVELNIG
                     HSIISRAVSVGLREAVREMLQLMEGYRN"
     gene            complement(3052..>3678)
                     /locus_tag="VG754_10160"
     CDS             complement(3052..>3678)
                     /locus_tag="VG754_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19623.1"
                     /translation="IGMTAPVSDAGKYMAQIGPRLAPALDSYFNEHALLGKAEWTNHQ
                     VRLSKLPFIAPYLAPTRDAKSDFLLGSLMPVRHTNSLVFPPELMRELLSKPNLVYYGW
                     EINEESIVQWQALQQIYLMDAVKPLPSNKSPGKDWLNAVKSKLGNCATEITLTAPNEL
                     TLERSGPIGLTGFEMSALEYWLDAPGFPLNAIYPRFNPSNLPKKQAVK"
BASE COUNT          796 a         1002 c          985 g          895 t
ORIGIN      
        1 gaagtctgat tcgggaagaa gccggtgatg ttcatagtcg gccagcaaca ttcctttgtt
       61 gccaacgaag agaacgccat ttttccaatc aagtatcttt ccctctttta taagttccgg
      121 ctgagtgccg ccatcatacc aagtgatctg gaccggagat ttcgaggcgt gtgtttcata
      181 ttcgtaacgt accacaagcc aggatggagt ggtctcagga ttaatcggcg gtcctttggc
      241 ttcgatggtc agcggatgtc gcaagtccaa cgcccagtag gcaagatcca tgtagtgaca
      301 acccatgtcg cccaatgtgc cgtttccgaa atcccaccag cgacgccggt tctttggaag
      361 atattcagaa tgataagccc gttccggcat gggtcccagc cacaaatccc agtgaagata
      421 agatggcaca ggcggactgc cttcaacgcg cccagtcccg ccccacgatt tctcgcacca
      481 gacatggcac tctgaaatgt tgcctatggc gccattgcgg atcaattcca ccacccgccg
      541 ataatttttt cccgcgtgga tttgcgtgcc catctgtgtg gcaagatttt tgtattgtcc
      601 ggcggtccgc gccaccaagc gcgattccga aacagtatgc gcaagcggct tttcgcaata
      661 gacatgacga ccggactgca acgcggcgat cgtggcaacg gcatgggtat ggtccgcagt
      721 gctgatgacg acggcatcaa gatcatttcg ctcaagcagt tttctgaaat cgttgtaggt
      781 tttcgcgccg ggaaatcttt gcgcggcggc ggacaaataa tggtcatcca catcgcacag
      841 cgcgacgatg ttctcctttt ggacaccgct gatgttggct tcggcgcgat tggcagtgcc
      901 gatgatgccg atgttcaggc ggttattggc tgaaaaactt ttcgcttttg aaagatgcgc
      961 gccaagccaa agcccggcgg ttgtcattgc ggttttgcgc agaaaatcgc gccgcgtcag
     1021 gggacttttt ttcaattcag ccataacttt tcgattaacc tctgatgata tccaccaaca
     1081 acggaccaac aaggtatcga ggggacaaat taattgtcgg aacttgtcct acttttggaa
     1141 accgaatccg tcacaagatt gtgttgcacg tacctttgct ttctttgtgg attcggctaa
     1201 cttggttgcg gcaactcaaa agctggattt gaatttatgc acaacgtcac ttcagtggag
     1261 ctaaagcggg attgcgaagt cgtgcaaatc ccgagcggca acccgatcct tttgcccgcg
     1321 ggcacgcccg tggacatcac ccaaaagttg ggcggcagtt ataccatcca tgcgcagggc
     1381 ggattgttcc gcgtctctcc cagcgatgcg gatgcgcttg gcttcgaagc ccccgctgcg
     1441 gctgaatcga gtgcgcaacc aggaggtccc gttgacgaaa agctcgtctg ggacacgttg
     1501 aagacttgtt acgatccgga aatcccggtg aacattgttg accttggtct ggtttacgac
     1561 atgcacattg aatcgctgcc gagcggcaac aatttgattt ccgtcaaaat gactttgaca
     1621 gcgcccggtt gcggcatggg ggcgaccatc gctggcgatg cccagcaaaa acttctttat
     1681 cttccaggcg tggaggaagc ggttgtggaa attgtctggg acccgccatg gcatcaatcc
     1741 atgatcaccg agcaaggccg gcgagttctt ggactttaaa ttgtttgaca aatcgcggat
     1801 gcgttcttaa gtttttgccg ctctggatgg ggtcgtagct caattggtag agcgccacaa
     1861 tggcattgtg gaggtcgcag gttcgatccc tgtcggctcc accatccttc gcacgaaagt
     1921 gtaacagttt aattctccaa aaccgccacc gccgtcgcat gttgctgcgt atgactgagg
     1981 ctgatcagca cgatgcgccc gccgcgttgt ttcaggagtt cttctccctt tccgtgcaga
     2041 atgacgtaag gttcgcccga ttcgcgtcgc gcaacttcca tgtcgtgcca gccaagttgc
     2101 gcgccgatgc cggtgccgaa cgccttggaa atcgcttcct ttgcggcaaa tcgcgcggca
     2161 agaaatggcg cgggcatttt gtgcgatagg caatacgcga tttcaccggg gtgcaggatg
     2221 cggttcagga agcgttcgcc aaatttttca tgcgaggcct ggatgcgggc cacttcgatg
     2281 atgtcgatgc cgattccgag gatcaattcc gatagccctc catcaattgc agcatttccc
     2341 gcactgcctc ccgcaatccc acactaacgg cccggctgat gatgctgtgg ccgatgttca
     2401 actcgacaag atgtggaacg gtgtgcagca ccggaagatt ttgataattc aatccatgcc
     2461 cggcgttgac cttgaggccc aatccatgcg cttgcttcgc tgcggcgatg aggcgttcca
     2521 attctatatt acgcgcggat ttctggtcaa aatgttcggc atacgctccg gtatgcaatt
     2581 cgatgaattg cgcgcccgcc ttcgccgagg cctcgacttg tgacgcatcg ggagcgatga
     2641 aaaaacttac ctcgatgccc gcatcgttca ttttcttgcg cgtctctttc aatgcttcca
     2701 aattgcctgc gacatcaagg ccgccttccg tggtgacttc ctgccgccgc tcgggaacga
     2761 ggcagacgat ttcgggtttt aactttaatg cgatgaccac aatttccggc gcattcgcca
     2821 tttcaagatt cagacgtgtc ttgatgacct cgcgcaactt ccaaacatcg cgatcctgga
     2881 tgtgccgacg gtcctcgcgc agatgcgcgg tgatgccgtg cgcacctgcc gattcggcca
     2941 tcaaagccgc agcgacgggg tccggctcgc catggcctgt gccgcgataa cgcgcctcgc
     3001 gcaacgtggc gacgtggtca atatttaccc ccagcttcaa catgggataa cttacttcac
     3061 agcctgtttt tttggcaaat tggacgggtt aaagcgcgga taaatggcgt tcagcggaaa
     3121 accgggtgca tcgagccagt attcaagggc gctcatctcg aagccggtca ggccgattgg
     3181 cccactgcgc tccaaagtca gttcgttcgg cgcggtcagt gtgatttccg tcgcgcaatt
     3241 gccaagtttg gatttgaccg cgttcagcca atccttgccc ggcgatttat ttgaaggcaa
     3301 aggcttgacc gcatccatca ggtaaatctg ttgaagtgct tgccattgta cgatgctttc
     3361 ctcgttgatt tcccaaccgt agtaaacaag gtttggcttg gacagaagtt cgcgcatcaa
     3421 ttccggtgga aacaccaggg aattcgtgtg tctaacaggc atcaagcttc ccaggagaaa
     3481 atcgcttttc gcatcccgag tcggggctaa atagggggca ataaaaggca gcttcgacaa
     3541 cctcacttga tgattcgtcc attccgcctt gcccagcaag gcgtgctcgt tgaaatacga
     3601 atcgagtgcg ggtgccaggc gcggcccgat ttgcgccata tacttcccag catcggaaac
     3661 aggcgcggtc atgccgat
//
LOCUS       DASZMM010000424         5661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2647518, whole genome shotgun sequence.
ACCESSION   DASZMM010000424 DASZMM010000000
VERSION     DASZMM010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647518"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VG754_10165"
     CDS             complement(<1..465)
                     /locus_tag="VG754_10165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWF19624.1"
                     /translation="MSLKLYLLRHGQTSFSRDNAFCGAGLNPELTTDGEAMAQCFAKA
                     YQALPWRAVYASPLRRTVATAKPLCEAIEAKMELRDDLMEIRYGKWEGKLVEDVDREF
                     HDDYIRWSADPAWNAPTEGEASVVIARRALQVIEEIKQRFDDGNVLVVSHKAT"
     gene            complement(462..1016)
                     /gene="lepB"
                     /locus_tag="VG754_10170"
     CDS             complement(462..1016)
                     /gene="lepB"
                     /locus_tag="VG754_10170"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWF19625.1"
                     /translation="MNTLEVSSQSTESVVRRFYNPEPEKRQFYIGLCILLWSILAYLF
                     ISHYVMMAVEIKGASMSPTLLDGQHYILYRCPYLWRAPRTGEIVVIHDPEDHQLSIKR
                     IIGLPNQLVEIRHDGVYVDNEKLAEPYLTAEAAWASGHKLIKSVRLGPNEFYVLGDNR
                     DRSADSRIYGSVPKNSILGLIHKP"
     gene            complement(1201..1779)
                     /locus_tag="VG754_10175"
     CDS             complement(1201..1779)
                     /locus_tag="VG754_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19626.1"
                     /translation="MKNILKILLCRFQLLSLALLAGVFCVSCASSRQVAIATPVPVIP
                     PNSVGSEWQSRGFLVVNTPAELHFDELSRRRFPHTGYSIYDSYTHKLLEYVPNRLGRA
                     GETPTQVLLPAGMYFVEARTPYHQPVLFPVLIRPQTETTVNLDNDVPPPNTITGDFYT
                     LQCRGRSIGWLEETAINAPPPIYPTAQLNPKN"
     gene            complement(2023..2268)
                     /locus_tag="VG754_10180"
     CDS             complement(2023..2268)
                     /locus_tag="VG754_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19627.1"
                     /translation="MDDLKMQDMILRALHENPNREAILVPSMFNPPIMLSNIFRLGGV
                     LKRKGYTTPPTRRMGGWHLRLLEPGVAYCEADPSKKK"
     gene            2472..3602
                     /locus_tag="VG754_10185"
     CDS             2472..3602
                     /locus_tag="VG754_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19628.1"
                     /translation="MTNNDSVTPKNIARRDFIKKVGYTAASTAVLAQTLPGLAQNAAQ
                     PIVVALVGCAHIHTPSYVGILKSRADVKVKCVWDHDRARAEKNAAQFGCPVVDDLKVI
                     WSDPEIKAVVICSETNRHRELVLAAADAKKHMFAEKPLGFTSEDSQAMASAVEKANVL
                     FTTGYFMRTDPQILFLKAQVEKENFGKITRVRASNCHNGSLGHWFDTDWRWMADPKIA
                     GVGAFGDLGTHKLDILMWMFGDVDAITADIKVVTGNYGDCDESGEALMKFKNGITGTL
                     GAGWVDVADPVTLLISGTEGHAHIDMGRLFFASRKVEGADGRRPWTDLPPALPLPMNQ
                     FIDAVGGKSSQTLVTPKEAAARVSVMEAAYKGARGQVWAKPA"
     gene            3775..5106
                     /locus_tag="VG754_10190"
     CDS             3775..5106
                     /locus_tag="VG754_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HWF19629.1"
                     /translation="MDPRNVITRALGVIHLCGALLVCGAAFAAQSSTNGLLKGQAALS
                     GDFSTDTPGVRRLITVNDLPIPYATKSVDNGPHMVPRPEGAMPKAPLGFVVDLLAANL
                     DNPRKIVTAPNGDLFIAEAGPGRIKILRQGPDGKIISTNIFAEHLRMPFGIAFFPPGN
                     HPKFVYVANTDSVVRYPYASGDLAATGPEEMIVPDIPGGGHLRGGGHWTRDIVFTRDG
                     KKMFVSVGSHSNGNDDEFEKNRADILQYNPDGTGFRIYASGIRNPVGLAIDSRTGNLW
                     TSVNERDNLGDNLPPDYITRVKENGFYGWPWFYLGNHLDPTHANKHPDVGAKTIVPDV
                     LLQAHSASLCLTFYTATQFPKDFHGGIFAAEHGSWNRERRTGYKVIFVPLKRGKATGE
                     YDDFLTGFVNQNGEVWGRPVGVTVDSQGSLIVVDDGSNNIWRVRYTGASAP"
     gene            5216..5455
                     /locus_tag="VG754_10195"
     CDS             5216..5455
                     /locus_tag="VG754_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19630.1"
                     /translation="MKTISTTELRSKTRSLVRSLENGKAVGLTHRGQKLAQIFPVKHA
                     NGIDADDPLYRFHRHASDKARPLSDREMDRLIYGG"
     gene            5445..>5661
                     /locus_tag="VG754_10200"
     CDS             5445..>5661
                     /locus_tag="VG754_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19631.1"
                     /translation="MAAEPLFIDSGGFYALISAESKFHKLAVTIMEEAARGHRRAVTT
                     DYIIDETATLLQARGLSKLLAEFFRMTE"
BASE COUNT         1292 a         1476 c         1591 g         1302 t
ORIGIN      
        1 cgtcgccttg tgggaaacga ccagcacgtt gccgtcgtca aagcgttgtt tgatttcttc
       61 gatcacttgc aacgcgcgac gtgcgatgac aaccgacgct tcgccttccg tgggcgcgtt
      121 ccatgccggg tcagcggacc agcggatgta atcgtcgtga aattcgcggt caacgtcctc
      181 caccaacttt ccttcccact tgccgtaacg gatttccatg aggtcatcgc gcaattccat
      241 ttttgcttcg atggcttcac acaatggttt tgcggttgca actgtccgcc gtaagggact
      301 ggcataaacc gcgcgccagg gaagcgcttg gtaagccttg gcaaaacatt gcgccatcgc
      361 ttcgccgtca gttgtcaatt cgggattcag tcccgcgccg cagaaggcat tgtcgcggct
      421 gaaggaagtc tggccgtggc gcagcagata gagtttcaga ctcaaggctt gtggatgagg
      481 cccagaatgg aatttttggg caccgacccg taaatgcggc tgtccgcgct gcgatcacgg
      541 ttgtcgccaa ggacataaaa ttcattcggg ccgagccgga ctgacttgat gagcttgtgc
      601 ccggaagccc acgcggcttc agcggtgaga tacggttccg caagtttctc attgtccacg
      661 taaacaccat catgccggat ttcgacgagc tgattgggaa ggccgatgat gcgtttgatg
      721 gaaagctggt ggtcttccgg atcgtggatg acgacgattt cgccggtgcg cggagcgcgc
      781 cagagataag ggcagcggta aaggatgtaa tgttgtccgt ccaacagcgt gggactcatg
      841 ctggcgcctt tgatctccac ggccatcatc acgtaatggc tgatgaacag ataggcgaga
      901 atggaccaaa gcaggatgca cagcccgatg taaaactggc gcttctccgg ttcaggattg
      961 taaaatctgc gcaccaccga ttcggtgctt tgtgatgaaa cctcaagcgt gttcatgtgt
     1021 gccaatcatt gagcgaaggg atttgtaacg cagcacccat cacaaaatca aggtgaaagc
     1081 cgcattggcc aaagattttt ttgtccgtaa ttattcggaa aaaatatgtg cgatgcaaag
     1141 cgacagactt tttttgctca aataattcaa ggcagtaact ctatggatca cgtgctcgat
     1201 tcaatttttt ggatttaatt gtgcggttgg gtaaatgggt ggtggggcgt taattgccgt
     1261 ttcctccagc cagccaatcg agcgtccgcg gcattgaagg gtatagaaat cgccggtgat
     1321 ggtgttcggt ggcggaacgt cattatcgag gttgaccgtg gtctcggttt gcgggcggat
     1381 gagcaccgga aagaggacag gctgatgata aggtgtccgg gcctcaacaa agtacatccc
     1441 ggcgggcaga aggacctgag tgggcgtttc acccgctctg ccgaggcgat ttggcacgta
     1501 ttccaaaagt ttatgggtgt aggaatcgta aatgctgtag ccagtgtgcg gaaaacgacg
     1561 ccgtgaaagt tcgtcaaagt gcagttccgc cggtgtgttg actaccaaaa agccgcggct
     1621 ctgccattcg ctgccaacgc tgttgggcgg aattacaggc acaggcgtcg ctatggccac
     1681 ctggcgagac gacgcgcagg agacgcagaa cacgccggcg agaagcgcaa ggctgagcag
     1741 ttgaaaccgg cacaggagaa ttttcaaaat atttttcata acaccccttt gacacgttgc
     1801 atactaacga acacagacgc gggaaccaat ggggtgctgc cctcgtggta aatggggcga
     1861 ggaaatttac gatccacaac atgcgattta cgccctcgaa taatatttgg aaaccgtttt
     1921 ctgattaagc cagaaacaaa ttattccgtg agcggtcttg tgctctctat tacgcaagca
     1981 gggccaaatc ttccggctgc gcgcaatctt aaatccgcaa attcattttt tcttcgacgg
     2041 gtccgcttcg caataagcga cgcccggttc gagaagccgc aaatgccatc ctcccatgcg
     2101 gcgcgtgggt ggcgtggtgt agcctttgcg ttttaaaacc ccgcccaacc ggaagatgtt
     2161 gctcaacatg atgggcgggt tgaacatcga ggggaccaaa atcgcttccc ggtttgggtt
     2221 ctcgtgtaaa gcgcggagaa tcatgtcctg cattttcaag tcgtccataa tggcattcct
     2281 ttcaatataa acgggtatga atgaaccttc gctgcaaatt caggatagga ttttggttgt
     2341 tctttatcaa tggaaaaacg aactttctgc acaggccgga gagttgacat ttccatgctt
     2401 gttaccgcac tgaatggcag gtagtctgat ttccaatacg cttgttaaat tttagaccac
     2461 tcgaatctgc tatgacaaac aatgactctg tgacgccgaa aaacattgcc cgtcgcgact
     2521 tcatcaaaaa ggttggttac accgctgctt caaccgccgt gctcgctcaa actttgccgg
     2581 gcctggcaca aaatgccgct caaccgattg ttgtggcgct tgtcggctgc gcgcacatcc
     2641 atacgccgag ttatgtgggc attctcaaaa gccgcgccga cgtcaaagtg aaatgcgtat
     2701 gggaccatga ccgcgcccgc gccgaaaaaa atgccgccca gttcggttgc cccgttgtgg
     2761 atgatttgaa agtcatctgg tccgaccctg aaatcaaagc cgtcgtgatc tgctcggaga
     2821 ccaatcgcca tcgcgaactg gtcctcgccg cagccgatgc caagaagcac atgttcgcgg
     2881 aaaaaccgct tgggttcacg tccgaggaca gccaggcgat ggcttcagca gttgaaaagg
     2941 cgaacgtgtt gttcacgact ggttatttca tgcgcacgga cccgcaaatc ctttttctca
     3001 aggcgcaggt ggagaaggaa aatttcggaa agatcacccg tgtgcgggcg tcgaattgtc
     3061 acaacggttc gctgggccat tggttcgaca ccgattggcg ctggatggca gacccgaaaa
     3121 tcgccggtgt cggcgcgttc ggcgatcttg gcacgcacaa actggacatc ctgatgtgga
     3181 tgttcggcga cgtggacgcc atcacagccg acatcaaggt ggtgacgggt aattacggcg
     3241 attgcgatga atcgggcgag gcattgatga aattcaagaa cggcatcacc ggcacgttgg
     3301 gagcgggctg ggtggatgtg gcggacccgg tgactttgtt gatcagcggc acggaaggcc
     3361 atgcgcacat cgacatgggg cgcttgtttt ttgcgagccg caaagtcgag ggcgcggatg
     3421 gtcgccgccc ttggacggat ttgcccccag cattgccgtt gccgatgaac caatttattg
     3481 acgcagttgg cggcaagtcg agccagacgt tggtgacgcc caaggaagcc gccgcgcggg
     3541 taagcgtcat ggaagccgct tacaaaggcg cgcgcggcca ggtatgggca aagcctgcgt
     3601 agcctcggga aacatccaac gccgaactgg aaattctgac attcaaagtt ctgcgttcga
     3661 tgttggatct ttgatgtttt cccgcccgaa tgacagcact cgttccagcc tcgaaacttg
     3721 acggagggcg gcgcgagggc cattttctat ccagaattta tgcctcaatc acgaatggat
     3781 ccccggaatg ttattacgcg cgcacttggt gtcattcacc tttgcggagc gctgctcgtt
     3841 tgcggagcgg cttttgcggc acaatcttca accaacggat tgctcaaagg ccaggcagcg
     3901 ttgagcggag attttagcac tgacacgccg ggtgtgcggc ggttgatcac ggtcaatgat
     3961 ttgccgatac cttacgccac caagtccgtt gacaatggcc cgcacatggt cccgcgtccg
     4021 gaaggcgcca tgccgaaagc gccgcttggt ttcgtggttg atttgctcgc agccaatctc
     4081 gataacccgc gcaaaattgt caccgcgccg aacggagatt tgttcattgc cgaagccggg
     4141 ccgggacgca tcaaaatttt gcgccagggg ccggacggaa aaatcatcag cacaaatatt
     4201 tttgccgagc atttgcggat gccattcggc atcgcgtttt ttccgccggg caaccacccg
     4261 aaatttgttt acgtggcaaa caccgattcg gtggttcgct atccttatgc aagtggcgat
     4321 ctggccgcga ctggtcccga ggaaatgatc gtgccggaca ttccgggtgg tggacacctg
     4381 cgcggcggcg ggcattggac gcgcgacatc gttttcactc gggacggcaa aaagatgttt
     4441 gtttccgtcg ggtcgcattc caacggcaac gatgatgaat ttgaaaaaaa ccgcgcggac
     4501 atcctgcaat ataatcccga tggcaccggg tttcgcatct atgcctccgg catccgcaac
     4561 ccggtcggat tggcgattga ttcccgcacg ggcaaccttt ggacttccgt caacgagcgc
     4621 gacaatttgg gcgacaacct ccctccggat tacatcacgc gcgtcaagga aaacggcttt
     4681 tacggctggc catggtttta tctcggcaat catttggacc caacccacgc caacaagcac
     4741 cccgacgtcg gtgccaagac catcgtgccg gacgtgctcc tgcaagcgca ttcggcctcg
     4801 ttgtgcctga ccttttacac ggccacacaa tttccaaagg actttcacgg cggaattttt
     4861 gcggccgaac acggttcgtg gaaccgcgag cgccgcaccg gctacaaagt catttttgtg
     4921 ccgttaaaac gggggaaagc caccggcgaa tacgatgatt tcctgactgg cttcgtcaat
     4981 cagaacggcg aagtctgggg gcggcccgtc ggcgtgacgg tggacagcca gggctcgctg
     5041 atcgttgtgg atgacggttc aaacaacatc tggcgcgtgc gttacaccgg agccagcgct
     5101 ccttgaaatc gcgaaattta aatacgcggc agtgtgtcgc tcaataaaat ttactcatgg
     5161 cttttgatgg gtgctatgct tggcaaagtg cttcgcttca tgatagactg ccattatgaa
     5221 aacgatttca accacggagt tgcgttcaaa gacacgctcg cttgtgcgct ctttggaaaa
     5281 cggtaaagct gtcggactca ctcatcgagg ccagaagctt gcccaaattt ttccagtgaa
     5341 gcacgccaac gggattgatg ccgatgatcc gctctatcgg tttcatcgtc acgccagcga
     5401 taaggcaagg cctttgtccg atcgggaaat ggatcggttg atttatggcg gctgaaccgc
     5461 tgttcattga ttcaggcggc ttctatgcat tgatttctgc tgaaagcaaa tttcataaac
     5521 tcgcggtcac aattatggag gaagctgccc gcgggcatcg ccgggctgtt acaaccgact
     5581 acatcattga tgaaactgcc acgttactcc aagcccgcgg tctttccaag ctgctggcgg
     5641 aattcttccg catgacggaa g
//
LOCUS       DASZMM010000425         8920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1574787, whole genome shotgun sequence.
ACCESSION   DASZMM010000425 DASZMM010000000
VERSION     DASZMM010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574787"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VG754_10205"
     CDS             <1..899
                     /locus_tag="VG754_10205"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HWF19632.1"
                     /translation="FNLLDDCWAMVETDHAKIENYFSLADSLKTETANAIWDQMLAMF
                     HTLDEFEQNRPGHANFQFYACRLLRPQFQRLGWKQKPGEPADDTLLRGKIISGLGHFG
                     DKTVIAEAKSRFEKFLTAPESLPPDLRPAVLKIVGRYSDRRAYDQIHQLALHAHGTED
                     RELCYNALCAALDPNLARQTLALSLTNEASPEEAVQLVIAVAATDGQTELAWEFARHH
                     LQELLAKVDPFSRNGYVPAIFSSFSDAARADELESFAGKNLSEDTLAKARETAAEIRF
                     KAALKKRVLPSIDLWTAGHIMN"
     gene            901..1218
                     /locus_tag="VG754_10210"
     CDS             901..1218
                     /locus_tag="VG754_10210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWF19633.1"
                     /translation="MNIQKITSGNGPAPKSGDTVVVHYTGWLTDGTKFDSSVDRDEPF
                     AFVLGTGQVIRGWDEGVASMKVGDKARLTIPPEMAYGAQGYPGAIPPNATLIFEVELL
                     SIQ"
     gene            2074..2388
                     /locus_tag="VG754_10215"
     CDS             2074..2388
                     /locus_tag="VG754_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19634.1"
                     /translation="MFVCRALLRLIEPRSVLTAERAAKAKQSISRMHPPVNGQQFRDE
                     ASLLNAQHGFVEKQGNPFFGHGLIGGLAAAQFGRAIGHADQAGIAEGGDQSLAARACR
                     GQ"
     gene            2761..5847
                     /locus_tag="VG754_10220"
     CDS             2761..5847
                     /locus_tag="VG754_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWF19635.1"
                     /translation="MNDENEFGREASPANGFGGRNGRGRDEQNGSARPSNGRASHPMK
                     YDPRNQREPAAKTIDGWFIAEILLPRWHWLFLSCCVLAFLGWAAGMLAWKKTYTATAQ
                     LRRFEPMASGDFFKPQPISSDMFASMLKSPELLQQVALNLDPPISPDLLSKCVLIKSD
                     SDSDMVKVLAKGYSQQFAVDTANVYATTAVDYTKELQRKEAEDINRVRILGPLTQMNG
                     QIADLEKKFRALPQSTLLTAKLNQLGGAITNLGAQLQNPAISYTAASRLTEKLLAAED
                     ELANLERKYTDEHPAVKQQKAAVAALQNEMAHSTNSSGQSLLNGPPTQLFNPDYEIIH
                     SQLAALDNSRQLLAARQQEAKAFIDDPPGNVQFFSPATLKDITPDHRWLKVALLTVFG
                     GVAGFLFALGGIFLVEFMDSRLKTSADVERVTKLPVIATLEELDGKSPSQREQWAFRT
                     WTMLQGRLSPSPNHGLVCGITSSTTGEGRSTWINLLADAASLSGFRVLTIATRPANGE
                     GTAPRELPNHRPGDPEADPNANALTTNALACPAEVTQQLTGPNAPPSVHIPLPGWVWN
                     LERRKQWLEALEHWRKVENLVILVELPPGSVPEAVLLGENLPNIVWLADSTKADAAET
                     RTQMETLRHARCNLVGAVLNRGPSAPLKSRFPRWLGSVALCVLATVVSANAEDTNVHY
                     APIPAASPAPAPATVAAPAPELAPAPANEIQTTNNAISSTNTNLSFSITTPSQRSEWQ
                     QRLTLGPGDLLNISLYGQPDLSRSEVLVGPDGRLNFLEAQNLLATGLTVDELRTNLDQ
                     ELGKYRRAPHTVITPVAYRSKRYYMLGRVVQRGVYVLDRPMTIIEAVARARGLEMGTT
                     GRNSIDLADFQRSFLMRHGKRIPLDFGNLFVRGDLSQNLAIEPDDYIYFPPADVKQVY
                     VLGEVRGAGPVPYTPTLTIIGALAARGGFTDVAYKTHVLVLRGSFSHPQGFVINTEDI
                     LYARGIDFKLQPKDIIFVSGRPFLHAEQLLDLAVSAFLQSVTVEATGRMMGSGLAQ"
     gene            5913..7019
                     /locus_tag="VG754_10225"
     CDS             5913..7019
                     /locus_tag="VG754_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HWF19636.1"
                     /translation="MANAWTGLFTLAMALTLLTGCQKPGPQFNPYSSRNRMNSDFETV
                     SLNRLDPALFRQSNASFTIGPGDRIEVELMDDATSRTDVTVGPDGKIYYNLLPGIDVW
                     GMTLAQVGEALQKGLSQFVREPPRVAVSLKTVESRRFWILGRVQSPGVYNMTNATTLL
                     EAIAIAGGSATLAGAKDIVGVGGNEDLADLRHTFIVRQGKILPVDFEALITGGDLSQN
                     VYLEPDDFIYFQPATAREVYVMGAVGQPNTIPYSGGMTMMSAISTCSGTVKEAYLSHV
                     AVVRGSLTEPKVAIVDYKAVLRGEVPDVLLQPDDIVFVPYEPYRYLVRYVNLIVNTFA
                     SSVAINGGILAVQPKAQTTTGVFIPAGSGVTVLH"
     gene            7188..>8920
                     /locus_tag="VG754_10230"
     CDS             7188..>8920
                     /locus_tag="VG754_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWF19637.1"
                     /translation="MRKIKFALLGIVSCLLMGQLSRAVEGTWEYAVQVSATVQTSPPQ
                     ITLSWPQDTTGTPSSYTVYRKTVDGTSWGTGTTLSGTTTTYVDNNVSVGSAYEYEIVK
                     VMPLYTGYGYIYAGINAPLTEARGKLVLVVDNTYSSQLTNELAQLQQDLIGDGWTVLR
                     HDVSRTDTVPNIKAVIQSEYNADPANVNAVFLFGHVPVPYSGDIAPDGHIPNHLGAWP
                     ADVYYGNMHGVWTDSSVSDSGADYSRNWNVPGDGKFDQSTIPGAVELMVGRVDLANLP
                     GELVWNGPATFPAELDLLRNYLNKDHKFRQNQMNLQRRGLVGDYFGIRNGEAFAASGW
                     RNFAPFFGAANVTSLPTEGTWIPTLASSGYLWSYACGSGSFSSIGGIGNSDAYFDGYS
                     TELYPANIQSAFTLLFGSWLGDWDSQDNIMRTVLASPTYGLTSGWSGRPHWFMHHMAL
                     GMPIGYSTRLTQNNGASGLYQNQINTAAGEIHVALMGDPTLRMHPVSPATAVTAKTNS
                     SSINLTWTPSTDSVVGYNIYRASNPNGPFTRLNNSLVSGASYTDSTANSGVYMVRAVK
                     LETSASGTYYNP"
BASE COUNT         1963 a         2495 c         2509 g         1953 t
ORIGIN      
        1 gtttcaatct cctggacgat tgctgggcga tggtggaaac ggaccatgcc aaaattgaga
       61 attatttctc tctggccgat tcgctgaaaa ctgagacagc gaacgcgatt tgggaccaga
      121 tgctcgccat gttccatact cttgatgagt tcgaacagaa ccggcccggc catgcgaatt
      181 ttcaattcta cgcctgccgc ttgctgcgac cgcaatttca acggctcggc tggaagcaaa
      241 aacctggcga acccgcagac gacactttgc tgcgcggcaa aatcatctct ggacttggtc
      301 acttcggcga caaaacggtc atcgcggagg cgaaatcgcg ctttgaaaaa tttttgactg
      361 cgccggaatc gctgccgcct gatttgcgtc ctgcagtttt gaaaattgtc gggcgatata
      421 gcgaccggcg agcttatgac caaatccatc aactggcatt gcacgcgcac ggcacggaag
      481 atcgggagct ttgttacaac gccctgtgcg cggcgctcga ccccaatctt gcacggcaga
      541 cgctcgcgct ttcgttgacg aacgaagctt cgccggaaga agctgttcaa ctggtcatcg
      601 ctgtcgctgc gaccgacggc caaacggaat tggcgtggga atttgcgcgc catcatcttc
      661 aagagttgct ggccaaggtg gatccgtttt cgcgcaatgg ttatgtgcct gcaattttca
      721 gttcgttttc agatgcggcg cgcgcggatg aactggaaag tttcgccggc aaaaatcttt
      781 ccgaagatac gctcgcgaag gcgcgcgaga ctgcggcgga aatccgtttc aaagctgcgc
      841 tcaaaaaacg cgtgcttccc agcattgacc tgtggacagc cgggcatatc atgaattgat
      901 atgaacatcc agaaaatcac ctcgggcaac gggcctgccc cgaaatcagg cgacacggtg
      961 gtcgttcatt acaccgggtg gctgaccgac ggcaccaaat tcgacagctc ggtggaccga
     1021 gatgagccgt tcgcttttgt attgggaaca ggacaagtca ttcgcggatg ggatgaaggc
     1081 gtggcgtcca tgaaagtcgg tgacaaggcg cgcctgacga ttccgccgga aatggcttac
     1141 ggcgcgcaag gctatcccgg cgccattccg ccgaatgcca cgctcatctt cgaggtcgaa
     1201 ttgctttcca tccagtaacg gtcccagaac ccatccgtct gcaaccgacc cgctcggcac
     1261 gaagaatttg ctgtatcgca gagtcgcact ctgcgcgaga attgaaaaca atggatgttt
     1321 ttgaaaattt ccacgttcaa cctccaatca tcgcgctgcc gactgcaagt cggcggcaca
     1381 gcagattgaa aatctgcgct acggcgcggc gcaagaaatt tcgtccggtg atcagcgctg
     1441 aaggcgcgat ttataccaga ctggggcaac gccccaggaa cacgcggaca attcagtatt
     1501 agggctgaag gcccgttcca atcaataggc agggaatctg atccatgatg agttttcggt
     1561 gtgaacgatg gagcgggctt tcagccctcg tgcatcggtg cctccttgca cccagggcgt
     1621 tgccctgggc tggtatgggg ccgggccttt ggccctcccg tgaaattcga agcaacctca
     1681 cgggaccctg ccagaatctt taggccaatt atccaatagt gacggtaacg gaaccgtaaa
     1741 caaagacgga tgggaggcaa atattatttt tcttgttata gcaatggagt cgtaacaagt
     1801 ggcgagacgg gctcggagct gactattgac ttaaacaggt tcgagaactg taccaatcat
     1861 agtacgcata gttggccgta agggtcatgc gagtgcctct tgggttcgca agtgtcaaag
     1921 agcgtcgtag atcaataagc gtatattttt ggggagcgcg gtcgatgtcc atccgaaagg
     1981 cctgcgcacg aatagcgtcg gcgagatgtc acggagcgcg agtcggcgac ccgcagcaag
     2041 ccgggatgga cggtgcctgt aaattagtct ggcgtgttcg tttgtcgcgc gttgctgcgg
     2101 ctcatcgagc cgcgctccgt tttgaccgct gaacgcgcgg caaaagccaa acagtccatt
     2161 tcacggatgc atcctccggt caatggccaa caattccggg atgaagcgtc acttcttaac
     2221 gcgcaacacg gcttcgtgga gaaacaggga aatccattct ttggccatgg tttgatcggc
     2281 gggctggccg ccgcgcagtt cggtcgcgcc atcggccacg cggaccaggc gggcattgcc
     2341 gaaggtggtg atcaatcgct cgcggcgcga gcttgtcggg gacagtaatc ggtttttcat
     2401 gggcgcattg tgcaacagga ggtgggacat aaactgtccg agatgattat ttagtttgtt
     2461 tcagagccgt ccgatgagga attaggaact caagaacagg ccctcattcc tcacgaattt
     2521 tcttcctgat ttcctgagtt cctaatgaaa aagtcttaag cgccatggac attcattttg
     2581 tcagccaacg cggtggggtc ttcggactgg gggattgcgg tcttcaccta cttctcggag
     2641 aagggcttcc gcatttaatc tcatgttcca tcggcgggga catgcctttg gacggcgcga
     2701 cactgtaaag ccgcgtcaac gatgaatgat tttgaaaaat gatgccaaca tgtccgctgc
     2761 atgaacgacg aaaatgagtt tgggcgagag gcttcccccg caaacggttt cggggggaga
     2821 aacggtcgtg gacgcgacga acagaatggt tccgcgcgtc cttcaaacgg tcgagcgtcg
     2881 cacccgatga agtacgaccc gcgcaaccaa cgcgaaccgg ccgcaaaaac gattgatggc
     2941 tggttcattg cggaaatttt gctgccacgc tggcattggc tgttcctgag ctgctgcgtc
     3001 ctggcctttc tgggatgggc cgccgggatg ctcgcctgga aaaaaactta cacggccacg
     3061 gcccagttgc gccgctttga gccgatggcg agcggagatt tcttcaagcc gcaaccgatt
     3121 tcctcggaca tgtttgcgag catgctcaag tcgccggaat tgctgcaaca ggtcgcgttg
     3181 aaccttgacc ctccgatttc tcccgatttg ctttccaaat gcgtgttgat caagagcgat
     3241 tcggattcgg acatggtgaa ggtgctggcc aagggttaca gccagcaatt tgccgtggac
     3301 acagccaatg tctatgcgac aacggcggtg gattacacca aggaacttca gagaaaggag
     3361 gccgaggaca tcaaccgcgt tcgcatcctt ggccccctga cgcagatgaa cgggcagatc
     3421 gccgatctgg aaaagaagtt ccgggcgttg ccgcaatcca ctttgctgac ggccaagttg
     3481 aaccagttgg gcggcgccat caccaatctg ggcgcgcaac tgcaaaaccc cgcaatttcc
     3541 tacaccgccg cttcgcgcct gacggaaaaa ttactcgcgg cagaggacga actggcgaat
     3601 ttggaacgga aatacacgga tgaacatccc gctgtgaagc agcaaaaggc ggcggtcgcc
     3661 gcgcttcaaa atgagatggc gcattcgacc aattcatccg gtcagtcgct gcttaacggc
     3721 ccgccaaccc aattattcaa ccccgattac gaaatcatcc acagccagtt ggcggcgctc
     3781 gacaacagcc gccaattgct ggctgcccgt caacaggaag cgaaggcgtt cattgatgac
     3841 ccgccgggaa acgtccaatt cttttcgccg gcgacactga aggacatcac gccagatcat
     3901 cgctggttga aagtcgcttt gctcacggtc ttcggcggag tggcaggatt tttgtttgct
     3961 ctgggaggaa ttttcctggt ggaattcatg gacagccggt tgaaaacgtc agccgacgtg
     4021 gagcgcgtga caaaattgcc ggtcatcgcg acgttggaag aactcgacgg aaaaagtccc
     4081 tcccagcgcg agcaatgggc gtttcgcacc tggacgatgt tgcaaggcag gttgagtccg
     4141 tcgcccaatc acggattggt ttgcggcatc acttcctcga ccaccggcga aggccgctcg
     4201 acgtggatca acctgcttgc cgatgcggcg agcctgagcg gatttcgtgt gttgaccatt
     4261 gccacgcgtc cggcaaacgg cgaaggcacc gcaccgcgcg aattgccgaa ccatcgcccc
     4321 ggcgacccgg aagccgatcc caatgccaac gcgctcacaa caaacgccct ggcttgcccc
     4381 gcggaagtga cacagcaatt gaccgggcca aacgcgccgc cgtcagtcca cattcctctg
     4441 cccggctggg tttggaacct tgagcggcgc aagcaatggc tcgaggcctt ggaacattgg
     4501 cggaaggtgg aaaatctggt catcctggtc gaacttccac ccggctccgt tccggaagcc
     4561 gtgctgttgg gagaaaattt gccgaacatc gtgtggcttg ccgacagcac caaggccgac
     4621 gccgccgaaa cgcgcacgca gatggaaacg ctccggcacg cgcgttgcaa tctggtcggc
     4681 gcggtattga atcgcgggcc ttccgcgccg ttgaaaagcc gttttccacg ctggctcggc
     4741 tccgttgctc tctgcgtatt ggcaacggtc gtttccgcaa acgcggaaga tacaaacgtt
     4801 cactatgcgc caatacctgc agcatcacct gcacctgctc ctgcaactgt ggccgcacct
     4861 gctcctgaac ttgcgcccgc gcctgcgaac gaaatccaga ccacaaacaa tgccatttct
     4921 tcgaccaata ccaatctttc gttctccatc acgactccca gtcagcgtag cgaatggcag
     4981 cagcgtctta ctcttgggcc gggtgatttg ctgaatattt cgctttacgg tcagcctgat
     5041 ctttcacgtt ccgaagtatt ggtcggcccg gatggacggc tcaatttttt ggaggcgcaa
     5101 aatcttttgg caaccggcct gaccgtcgat gagttgcgga ccaaccttga tcaagagctt
     5161 ggcaaatatc gtcgcgcgcc gcataccgtc atcacgcccg tcgcatatcg cagcaaacgt
     5221 tattacatgc ttggccgcgt cgttcaacgc ggggtttatg tgctcgacag gccgatgaca
     5281 atcatcgagg cggtcgcgcg ggcgcgcggc ctggaaatgg gcaccaccgg ccggaacagc
     5341 attgaccttg ctgatttcca gaggtccttc ctgatgcgcc acggcaaacg cattccgctt
     5401 gatttcggaa atctttttgt gcgcggcgac ctttcccaaa acctggcgat cgaacccgat
     5461 gattatatct actttccgcc ggcagacgtg aagcaggttt acgttttggg agaagtccgc
     5521 ggcgccggcc cggtgcctta caccccgact ctaacgatta ttggagcgct tgcggcccgt
     5581 ggcggcttta ccgatgtggc atacaaaacg cacgtgttgg ttttgcgcgg ctccttcagc
     5641 catccacagg ggtttgtgat aaacacggaa gacatcctct atgcgcgcgg aatagacttc
     5701 aaactgcagc cgaaagacat catttttgtc agcggccggc cattccttca cgccgaacaa
     5761 ctgctcgatt tggccgtcag cgccttcctt caatccgtga ccgtcgaagc gacaggcagg
     5821 atgatgggca gcggacttgc acaatgaaaa cattcgttct tacgcaatat tgtatgcacc
     5881 cgctgcccaa gcgtggcggg gttgctaaac acatggcaaa tgcatggacg ggtctgttca
     5941 cgctcgcgat ggccttgacg cttttgacgg gatgtcaaaa gcccgggccg cagttcaatc
     6001 cttattcgtc tcgcaaccgg atgaattccg attttgaaac tgtcagcctc aatcggctcg
     6061 atcctgcatt gttccggcaa tccaatgctt cgttcacaat cggcccggga gaccggattg
     6121 aagtggagtt gatggatgat gcgacttcga ggacggatgt caccgttggt ccggacggga
     6181 aaatctatta caatctgctg ccgggaattg atgtttgggg aatgaccttg gcgcaagtgg
     6241 gcgaagcgtt gcaaaaggga ttgtcgcaat tcgtccgcga accaccgcgc gttgctgttt
     6301 ctttgaaaac ggttgaaagc cgccgcttct ggattttggg ccgtgttcaa tcgcccggcg
     6361 tttacaacat gaccaatgcg acgactttgt tggaagcgat cgccattgcc ggcggctcgg
     6421 caactcttgc cggggcaaaa gacatcgtgg gcgtcggagg aaacgaggac ttggcggatt
     6481 tgcgccacac tttcatcgtg cggcaaggaa agattttgcc tgtggatttt gaggcgctga
     6541 tcaccggcgg cgacttgtcg caaaacgttt atctcgaacc ggacgacttc atctatttcc
     6601 agcctgcgac cgcccgggaa gtttatgtga tgggcgctgt cggccagccg aacacaatcc
     6661 cgtactcggg agggatgacg atgatgagcg cgatttccac ttgttcggga acggtgaagg
     6721 aagcgtatct ttcgcatgtg gccgtcgtcc gcggctcgct cacggagcct aaagtggcca
     6781 tcgttgatta taaagccgtc ctgcgcggtg aagttccgga cgtgctgttg cagccggatg
     6841 acattgtttt cgttccgtac gaaccgtatc gttatcttgt tcgatacgtg aatctcatcg
     6901 tgaacacgtt cgccagttcc gtcgcgatca acggaggcat cctcgcggtg caaccgaaag
     6961 ctcaaaccac aacaggcgtg ttcatccctg cgggcagcgg cgtgactgtt ctccattaat
     7021 attagtggag agatttcgca ggactgatct cgcagaaaac gttcttcgac aagatttcgc
     7081 ggtgtttttt tgtaggcatg acactgacgc ctgcgaactc ctcattccga caggataata
     7141 agaggcacgc cgattttgcg tacttgtgag ccagacatac tggccacatg cgaaaaatca
     7201 aattcgccct tttgggtatc gtatcgtgtt tgttgatggg tcaactgagt cgtgccgttg
     7261 agggcacctg ggaatatgca gtccaggtga gcgccacggt gcagacttcg ccgccccaga
     7321 tcaccttgag ctggccgcaa gacaccaccg gcactccatc cagctacacg gtttaccgca
     7381 aaactgtgga tggcacttcc tgggggacgg gcacaaccct ttccggcacc acgaccacct
     7441 atgtggacaa taacgtgagc gtcggctccg catacgaata tgaaatcgtc aaagtgatgc
     7501 cgctatacac cggttatgga tacatctacg ccggcatcaa cgcgccgttg actgaagcgc
     7561 gcggcaaact ggtgctcgtg gtggacaaca cctattccag ccagttgacg aatgaattgg
     7621 cgcaattgca acaggacctc atcggtgacg gttggactgt gttgcgccat gatgtttccc
     7681 ggactgatac cgtgccgaac atcaaggccg tgatccaatc cgaatacaac gcggacccgg
     7741 cgaatgtgaa tgccgtgttc ctgttcggcc atgtgcccgt tccttattcc ggcgacatcg
     7801 ctccggacgg acacatcccg aaccatctcg gcgcatggcc ggcggatgtt tattacggca
     7861 acatgcacgg cgtttggacc gattcgagcg tgagcgacag cggcgcggat tattcgcgca
     7921 attggaacgt gccgggtgac ggcaagttcg atcaatccac catccccggc gccgtggaac
     7981 tcatggtcgg tcgtgttgac ctggcgaacc tgcccggaga actcgtctgg aatggcccgg
     8041 ccactttccc ggctgaattg gatctgctcc gcaattattt gaacaaggac cacaagtttc
     8101 gtcagaacca gatgaacctg cagcgacgcg gtctggtggg cgattatttt ggcatccgaa
     8161 acggtgaggc gttcgccgcg agcggttggc gcaatttcgc gccgttcttt ggcgcggcga
     8221 atgtcacttc gctgccaacg gaaggcactt ggattccgac actggccagt tcgggttatc
     8281 tttggtcgta cgcgtgcggc tccggcagct tcagcagcat cggcggaatc ggaaacagcg
     8341 acgcttactt tgacggttac agcaccgagc tttatccggc gaacatccag tccgcgttca
     8401 cactgctgtt cggcagttgg ttgggcgatt gggattcgca agataacatc atgcgcaccg
     8461 tgctggcgtc gccgacttat ggattgacct ccggttggag cggccgtccg cattggttca
     8521 tgcaccacat ggcattgggc atgcccatcg gctacagcac gcgtctcacg cagaacaacg
     8581 gggcaagcgg tctttatcaa aaccagatca acaccgccgc cggcgaaatc cacgtcgcat
     8641 tgatgggcga cccgacgttg cgcatgcatc cggtttcgcc cgcaaccgct gtgaccgcca
     8701 agaccaattc cagcagcatc aacctgacct ggaccccttc gacggattca gtggtcggct
     8761 ataacattta ccgcgccagc aatccgaacg ggccgttcac gcgcttgaac aactcgctcg
     8821 tttccggcgc ttcttacaca gattcaaccg ccaattccgg cgtttacatg gtgcgcgccg
     8881 tcaaattgga aaccagcgcc agcggcacat attacaatcc
//
LOCUS       DASZMM010000426         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4583474, whole genome shotgun sequence.
ACCESSION   DASZMM010000426 DASZMM010000000
VERSION     DASZMM010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4583474"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VG754_10235"
     CDS             complement(<1..547)
                     /locus_tag="VG754_10235"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWF19638.1"
                     /translation="MTPSIPVTGAASLKTDIPVKLNVAIIVTQLAAILLCCYAATRVH
                     HALSLGGLALAFGILMNSVYAIIHEAEHAMLFPNRRLNDLAGAAMALFFPAPFHLIRQ
                     GHLGHHFRNRSDDEAFDLYFEGDHRLWRYLILYGILTGCYWIVVVLGNLIFLFFPFAA
                     DKKYWDFDRPSHAFMESLNPSY"
     gene            721..2109
                     /locus_tag="VG754_10240"
     CDS             721..2109
                     /locus_tag="VG754_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006684992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF19639.1"
                     /translation="MLEGKLKIALISPKGPLYRHRGGIWKKSLRYQPLTLTTLAALVP
                     AEIPVELQLFDEGIHDVPLDLDADLVGLTVITGTAMRAYELADHFRRRGITVVLGGPH
                     VTLIPEDAQPRANVIVVGYAEDTWPQLIRDFAGGCLQSRYDQEPNLDLANRAFARRDL
                     LPSRHYLTNNVFEATRGCIHNCDFCVVPAAWGRKPYQKPAQDIVADIRQHGARKLIFV
                     DLNLIADRAYAMELFTALIPLQVQWYGLATVLLADDLQLLELAARSGCRGLLMGLESI
                     SPQNLRNSHKGFNAPERFARVVDVLHQHGIALQGCFVFGLDHDEPDAFLKTAEFAVQA
                     RIDLPRFAIVTPFPNTALYKRLEAEGRILTKNWELYDGQHVVFRPAKLSIEELQSGTE
                     AAWKHAYSFRSIARRITHSPASWPVKLGTNLGYRFYAHNLSRFYNCDWVIDRAPARQA
                     SAPNPLTVSPGH"
     gene            2254..3465
                     /locus_tag="VG754_10245"
     CDS             2254..3465
                     /locus_tag="VG754_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF19640.1"
                     /translation="METIPFDEATDLVAISVETYTAKRAYQIASEYRKRGVPVVMGGF
                     HASLCPDEVSRHAEAVVTGEAEAVWPTVIDDARHGTLKKFYRQSSRVSLTGLKPNRSI
                     FRGKRYLPIGLIEAGRGCHFKCEFCAVQTVFNSTQTRRPVDDIIAELRLLKGEKKLFF
                     FVDDNITSNLEQAKDFFRALIPLGIRWVSQSSINAAHDEEFLDLLVRSGCQGVLIGFE
                     SLNPINLKEMNKAFNTMRGGFEQALANLRKHRVRVYGTFIFGYDADTPESFQKTVAFA
                     KDHALYIAAFNHLTPFPGTPLYRRFETEGRLLYDAWWLDDRYSYNRIPFQPRNMSPEN
                     LQKNCVGARRDFYSWRSILERGFDQVNRSDWFMWRNFYLINAMHRTDVSLRDHYPLGD
                     ESWQGQLLTAN"
     gene            3474..>3844
                     /locus_tag="VG754_10250"
     CDS             3474..>3844
                     /locus_tag="VG754_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19641.1"
                     /translation="MSGQVRFTLADERHNGAIRRLLRDNPMPGEVTLTLEREPDYFLG
                     SRLPGALEDQTILALEGERVVCMGGCSVRERYINGRCQSVGYLGGLRLDHSVQGRFDI
                     VRRGYKFFAEIAQYPPPDYYFT"
BASE COUNT          895 a         1043 c         1021 g          885 t
ORIGIN      
        1 ggtacgacgg gttgagagat tccatgaatg cgtgtgacgg ccggtcaaaa tcccaatatt
       61 tcttatcagc cgcaaaaggg aaaaacagga agatcaaatt tcccaggacc accacaatcc
      121 agtaacaacc ggtcagaatt ccgtagagta tcaaataccg ccataatcga tggtcgcctt
      181 caaagtaaag gtcgaaagcc tcatcatccg accgattgcg aaaatgatgc ccgaggtgcc
      241 cctgtcgaat aaggtggaaa ggcgcaggaa aaaacaacgc catggcagcg cctgccaggt
      301 cgttcaaacg acgattggga aaaagcatgg cgtgctcggc ttcatgaatg atggcgtaca
      361 ccgaattcat caagatgcca aaggcaagcg ccagaccgcc gaggctcaag gcgtggtgga
      421 cgcgcgtggc ggcatagcaa caaagaagaa tcgcagcgag ttgagtgaca atgatcgcca
      481 cgttcagttt cacgggaatg tccgttttta aggacgccgc cccggtgacc gggatactgg
      541 gagtcatggt tggcacaaca acaaacgatt acaaattgcg cccaagcata caatttcaaa
      601 aatcaggggc gacatcaaaa aaatcgcgcg gggcatcgaa tttatcaatc gccgctcccc
      661 gctattccac ttgtagcgtc cactggtgcg gcataagact aatgcaaaca caggcactcg
      721 atgttggaag gcaaactcaa aatcgcgctc atctcgccga aaggtccact ctaccgccat
      781 cggggcggaa tttggaaaaa gtcgctccgt tatcagccgt tgaccctcac cacgctcgct
      841 gcgctggttc cggctgagat ccccgtcgaa ctccagcttt tcgatgaagg cattcatgac
      901 gtgcctttgg atctggacgc tgacctggtc ggtctgaccg tgatcacggg cacagccatg
      961 cgggcgtacg aattggccga tcattttcgt aggcgcggta tcacagtcgt gctcggcggt
     1021 ccacacgtca ctttgattcc agaagacgcc caacctcgtg ctaacgtcat cgttgtcggg
     1081 tatgccgagg acacttggcc gcaactgatc cgcgactttg ccggtggatg cctccagtcg
     1141 cgctatgacc aggagcccaa tttggacctt gcgaatcgtg cgtttgcccg gcgcgatttg
     1201 ctgcccagcc gtcattatct cacaaataac gtgtttgaag ccactcgcgg ttgcattcac
     1261 aactgcgact tctgcgtcgt gcccgccgcc tgggggcgca aaccgtatca gaaaccagcg
     1321 caagacatag ttgcggatat tcgtcaacac ggcgctcgca agcttatttt tgtggatttg
     1381 aatctcattg ccgatcgggc ttacgcaatg gaattattca ccgccctgat tccgctccaa
     1441 gtccaatggt atggcctggc cacggtgttg cttgctgacg accttcagtt gctcgaattg
     1501 gcggcccgta gcggatgtcg gggactactg atgggattgg aatcaatttc cccgcaaaat
     1561 ttgcgcaata gccacaaagg cttcaatgcg cctgagcgtt ttgccagggt ggttgatgtg
     1621 ctccaccagc acggcattgc tctccaagga tgtttcgttt tcggactcga tcacgacgag
     1681 cccgatgcat ttctgaaaac cgccgagttt gcggttcaag cccgtattga tctcccccgg
     1741 tttgccattg tcacgccatt tccaaatacg gcactatata aaaggttgga ggccgaaggt
     1801 cgaatcctga cgaagaactg ggaattgtat gacggccagc acgtcgtttt ccgtccagcc
     1861 aaattgagca tcgaagaact ccaatcaggc actgaggctg cgtggaagca tgcgtatagt
     1921 tttcggtcca tcgcgcgtcg catcactcat tcacccgcct cctggccggt gaaacttggc
     1981 acgaatcttg gctaccgttt ctacgcccac aatctgagcc gcttttacaa ttgcgattgg
     2041 gtcattgacc gcgctccagc gcggcaagcg tcggcaccca acccactgac tgtttctcca
     2101 ggtcattaaa tgaggctcac cattatccat ccctgcatcg gacgccgccc ggggcagtct
     2161 tatattcgga cctggcagat ggagcctttg ccggcagcaa cgctcgctgg ccttacaccc
     2221 aatgatgttg agcttaagtt ttatgatgac cgaatggaaa ccattccatt tgatgaagcc
     2281 acggatctgg tggccatcag cgttgaaact tataccgcca agcgcgccta ccaaattgct
     2341 tctgaatacc gcaaacgcgg agttccggta gtcatgggag gatttcatgc gtcactttgt
     2401 cctgatgagg tttcacgaca tgcggaggcc gtcgtgactg gagaagcgga agctgtctgg
     2461 ccgacagtga tcgatgatgc gcggcatgga acattgaaaa agttttaccg ccaatccagc
     2521 cgggtttcac tgacaggtct caagccaaac cggtccattt ttcgaggcaa gcgctatttg
     2581 cccatcggat tgatcgaggc cggacggggg tgccatttca agtgcgaatt ttgcgccgtc
     2641 caaaccgtct tcaactcaac acaaacccgc cggcctgttg acgacatcat tgcggaactc
     2701 cgcctgctga aaggcgaaaa gaaacttttc tttttcgtgg atgacaacat cacgtcaaat
     2761 cttgagcagg cgaaggactt ttttcgggcc ttgataccgt tggggattcg ttgggtaagc
     2821 caatccagca tcaacgcggc gcacgatgaa gaatttctgg acctgctcgt gcgcagcggc
     2881 tgccagggtg tgttgatcgg ttttgagagt ctcaatccca tcaatctcaa ggaaatgaac
     2941 aaggcattca acactatgcg cggtggcttt gaacaggcgc ttgccaactt gcggaagcat
     3001 cgcgtccgag tttacggcac gttcattttt ggctatgatg cggacactcc ggagtctttc
     3061 caaaagacgg ttgcgttcgc caaagatcac gccctgtata tcgccgcatt caatcatctg
     3121 acaccatttc ccggcacccc gctataccgg cggtttgaaa cggaagggcg actgctttat
     3181 gatgcgtggt ggttggacga ccgctacagc tacaatcgaa ttccctttca accgcgcaac
     3241 atgagtccgg agaatttgca gaagaattgc gtgggcgcac gccgggattt ttactcatgg
     3301 cgcagcattt tagagcgtgg gtttgaccag gtaaaccgca gtgattggtt catgtggcgg
     3361 aacttttacc tgatcaacgc catgcaccgc accgacgtga gcttgcgcga ccattatcct
     3421 ttgggggacg aatcatggca gggacaacta ttaaccgcca actgagcgcc gtcgtgtctg
     3481 gccaagtccg ctttaccttg gcggacgaac gacacaacgg agcaatccgt cgcctgctgc
     3541 gcgataatcc catgcccggc gaagtgaccc tgacacttga gcgcgagcct gattattttc
     3601 tcggttcgcg attaccggga gcgttggagg accagaccat tctggctctt gaaggcgagc
     3661 gcgtcgtatg catgggcggc tgctcagtac gcgagcggta tataaatggc cgttgtcaaa
     3721 gcgtcggata tcttggcggg ttgcgtctgg atcattcggt tcaaggacgt tttgatattg
     3781 tccgccgcgg ttataagttt ttcgcggaaa tcgcccagta cccaccgccg gattattatt
     3841 tcac
//
LOCUS       DASZMM010000427         8810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_749297, whole genome shotgun sequence.
ACCESSION   DASZMM010000427 DASZMM010000000
VERSION     DASZMM010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749297"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..3191
                     /locus_tag="VG754_10255"
     CDS             438..3191
                     /locus_tag="VG754_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF19642.1"
                     /translation="MRVFLGLLFFSIAMGFSQWVDATQWIDADVCVYGGTSGGVIAAV
                     QAAQMGKKVALVVVNNHLGGMTSGGLGQTDLGSLGTGYIQGMTREFYTRVGQKYGTGA
                     TYTFEPHVAEAVFNDMVQQAGVQVYTNQYLVSVITQADNSIASVTMNNSNVFRARMFI
                     DASYEGDLMAKAGVSYTVGRESSSQYGESLNGARTPNTSDHQFGSISINPYIVSNNPA
                     SGLLPLIETNSAPTAGSADQRVQACNFRMCLTQNATNKIPITAPTNYSAAQYELLARY
                     IQALIAQGSTPTLANFMGIYVMPNGKTDINNNGPVSTDFIGQSDSYLEADAAMRAQLW
                     QSNKNYLQGFFYFLETDPRVPASLSSAMQAYGLCRDEFTDNGGWPCQLYVREARRMVS
                     DYVLTQSNCVGLVTVPDSIGLAAYAMDSHNCERIVVNGSVENEGDTYSVGGISSFWPI
                     PYRAIVPKTGQCPNLLVPWCISASHIAFGSFRMEPVFMIVSQSAGTAACMAIDANVPV
                     QQLDYGKLRLQLLANQQALGSSVVTNSGIVQDNADPTGVTITGQWPSSTSIAGYYGTN
                     YITDNNTNKGTKSVRFTPAIPSTGSYQVYLWWTADYNRATNVPVDVISAAGTNTIIIN
                     QTQNGQQWSYIGTFNFTAGTSNSVLIRTTGTSGYVIADAARFVPAAQASSGVDIISTC
                     GQAAKWQTLPGWFTLVRAGSTNSPLTINYTVGGTASNGVDYLPLNNSVTIPAGSAAST
                     VTVIPIANGLIEGNRTVVLTLNSSTNYSFDAYTNATITIQDTPLAQWALAHFTSAQLT
                     NAAISGDNADPNQDGLPNLGDYALGLDPLAANNSGLPIFSIQNGFFTLAYLRADGAVD
                     VTSSVEMSTNLLNWNCGSNHVSDSAINDDGTNQTRIAQALLPTASQPMCFFRFKFARK
                     QSP"
     gene            complement(3233..3883)
                     /locus_tag="VG754_10260"
     CDS             complement(3233..3883)
                     /locus_tag="VG754_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF19643.1"
                     /translation="MSEVMPRRVSKKIEIMVVDDHFVVRVGLRGSIDLESDMAVMWEA
                     STGEQAVSMYRQNQPDLVLMDLNLPLASGIDATKAICNEFPDAAIIILSTHEGEEDIY
                     QSFHAGAKGYIVKTAAREELINAIRVAAAGDRYIPAAIGARLAERMTHPDLTSREMEV
                     LELIVAGKSNKEIASELSITEVTVKLHVGHLLTKLRVHDRTQAATTALQRGIIHID"
     gene            complement(3936..4844)
                     /locus_tag="VG754_10265"
     CDS             complement(3936..4844)
                     /locus_tag="VG754_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF19644.1"
                     /translation="MKLTYRNDDSTASPGEGEKMQFGFWARAWHRREGFTLIELLVVI
                     AIIAILAGLLLPALARAKSKARQTSCLNNERQLQIAYYMYTDDSGGVLANNDTGNGLS
                     AGPNAWIQGNVQQCNANYTNNIMTGVLWRYNGNTDIYKCPASHASVAAPVGNGTVPHN
                     RSYSISAQLNCPTWGLNDAYTSVAIKDSDVQQPSQVAVFVEENQFSIDNGTLGIASLA
                     TPEFWNPPSNRHNNGAIISFMDGHSERWAWTGSKLPGINAANNSPDETCPSSNRSSPN
                     SNPLSHIATTSNDPDYVRLANALPQP"
     gene            complement(4841..7090)
                     /locus_tag="VG754_10270"
     CDS             complement(4841..7090)
                     /locus_tag="VG754_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19645.1"
                     /translation="MNKPSTSRTLWKSAPVCAALFLLASTAAQAQFLTNGDFEAAPIG
                     SGTTVFPGWANTSGTVSDSPVIITGSHSAKCVLTSAGYLRQSPSDPGGLLTNFIFECD
                     FACATNSSSRTFQLNLYHNGTAANINLIIITNAVRLISGSSTANNVLKGVIDYMPDVN
                     TVHVNHLKIVGRYTDPTPNYDVIITNSLGSNAVLNQTFFQNAAPSGTNDRPTLISFEC
                     GNLASGGTNILVVDNVSLTNSSGGSGVHFAATISSQPTPTNLVVLQGYTNLTIAAGIG
                     GDAPLSPQWQFNGVNINPAVNPSATNNNGTNSTLSFTNVTPASAGNYDLHLTNAYGGA
                     TSSVVTLTVSPVLNTSRMSNVWTLLPGQRPYLTNTGSGSSPTERGMAYNPVSSNLLLA
                     SRAGSTNMIVALDPSTGAQRYFLNTGNLGAADSFSSPLNEIGVADDGVVYAANVTTQA
                     GTANFILYQWSDDSTNSTPSRCYTGDPGDPVAPNMRWGDNMAVRGAGVNTQILLAPGS
                     GTNIGIVALMTTPDGFGFNPTIITVAGVSNSAFAEFGLAFGPGTNTFWAKGANQQLQL
                     VQFDPNSGTGTLLDTYSTNSVLPNSGPLGANAAQNLLAVLTFETPDDVRLYDVSNPSA
                     DPVLLDQKLFPVKNPNAVNGLPGAGAVIFAGNQLFVLDSNNGIQAFQVNTNQSLATFP
                     LTIQAQPGGGVLLTWQSVSGHNYQVQYKNNLTDAGWTNLGSQITASSTSTSTTDTPGT
                     TRFYRIQGQ"
     gene            7276..>8810
                     /locus_tag="VG754_10275"
     CDS             7276..>8810
                     /locus_tag="VG754_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19646.1"
                     /translation="MSTIARARNAVPGPLNPVFDKCCNRISHGGIFGIIIAALTIFCP
                     CAPAESTGSVLWGVMDWKLDALPPATDTSTTSPLYGDGNCRIICHQYQSADWLPFKIS
                     TTNEMEISRIYGKGSCINLMLYFVSCTHDTNSNTFSYPLNSQFLSDFTQLVNAYKVGG
                     GPLYIQVFPEFEEWYRTSSTNEANQYRAQITAQFLAMAQIAHTNYNRAYIGLCFEGGD
                     FTGDTSQFTNRWNTILTSSDLVFVNFMSPFTQANTDERAMVLGTQILGNWGLPMMFPY
                     VDLWDDNNTPIFTVGDNYTNDATAFSGYITNWIGSALTAPAEVRGSSSTNWTTNLRTL
                     KSRGLFAFSLYRASWCNNSVTPPDPNGATHPLLAYNILTNAMATNSRAFLYPITEMEY
                     WPSNHPPGASTTYEIQTDTNCSSGQLVVLDSTNATGQYFTYRAPVTHNGTYEVYIGIR
                     KQTDGGWFYLYVNGSVSQLGSERNCSLATNVGNYQEYDQGAVTFSNLTGTTWTDFKFG
                     VSRA"
BASE COUNT         1944 a         2350 c         2486 g         2030 t
ORIGIN      
        1 gtccgctcat gatggcccgc agtattcgcg cattcaaagt caatcacaca ctgtgcagac
       61 caactgcaga acaatgaaat ccgagttgag ttattctgcg attgagcgac ctttctactg
      121 gaggatcatc aatttaattt agcggtcgaa cgcagctaag tggagtgctg taatagtttt
      181 tcccaggacc cacccgcaaa cggagcgata atagggcgtt ggactttgca tctgcggtgc
      241 tcagatggtg aatcgcgcat tggcaactat actaaagccg tagttcgcca ctcgtccatt
      301 ggcgtcgtga attttgcgca cgtatatggc atgttggcag cgaaccattg acaaacgggc
      361 cttaacgggc tgtagcgcga tttaacatga tgtaccattt ttcccggccc gtccgccgat
      421 tcttaattgc ccgacagatg cgagttttcc tcggattgct atttttcagc atcgccatgg
      481 gtttctcaca atgggttgat gccacacaat ggatcgatgc ggatgtttgc gtttatggag
      541 gcacctccgg cggagtgata gcggcagtgc aggccgccca aatgggcaaa aaggtggcct
      601 tggtggtggt caacaatcat ttgggcggga tgacttccgg cggcttggga cagacggatc
      661 ttggctcgct tggcacagga tatattcaag gaatgacccg ggaattttat acgcgtgtcg
      721 gtcaaaaata cggcacgggc gcgacttaca cattcgagcc gcacgtcgcc gaggcggtct
      781 tcaacgatat ggtccaacaa gctggcgttc aggtttatac caatcaatat ctcgtctcgg
      841 tcatcactca agcggacaat tccatcgctt cggtcaccat gaacaacagc aatgtttttc
      901 gggccaggat gttcattgac gccagttacg agggcgattt gatggcaaaa gcaggagtaa
      961 gctacacagt cgggcgcgaa tcctcctcgc agtatggcga gagtctcaac ggtgcgcgga
     1021 caccgaatac gagcgatcac cagtttggca gtatttccat aaatccatac attgtctcca
     1081 acaaccccgc gagcggcttg ctgccgctca tcgagaccaa cagcgcgccg acagcgggca
     1141 gcgccgacca gcgggttcaa gcctgcaatt tccggatgtg ccttacacaa aacgcaacga
     1201 ataaaattcc gatcacggcg ccgacaaatt acagcgccgc gcagtatgag cttctggccc
     1261 gttacatcca ggcgctgatc gcccagggca gcacgccgac actcgccaat ttcatgggca
     1321 tttatgtcat gcccaacggc aaaacggaca tcaataacaa tggcccggtt tcgactgatt
     1381 tcattggcca aagcgattca tacctcgaag cggatgccgc gatgcgcgcg caactctggc
     1441 aatcgaacaa aaattatctt cagggatttt tctactttct cgaaacggac ccgcgcgtgc
     1501 cggcgagttt gagcagtgcg atgcaggcgt acgggctgtg ccgcgatgaa ttcacggaca
     1561 acggcggctg gccttgtcag ctctacgtcc gcgaagcccg ccggatggtt tcggactacg
     1621 ttttgacgca atccaattgc gtgggtttgg tgaccgttcc ggattcgatt ggattggcgg
     1681 cttacgcgat ggattcgcac aattgcgagc gaattgtcgt gaatggctcg gttgaaaacg
     1741 agggcgacac ttattcagtg ggcgggattt cgagcttttg gccaattccg tatcgcgcga
     1801 tcgtgccaaa aacgggccaa tgtccgaact tgctggtgcc gtggtgcatt tcggcttcgc
     1861 acatcgcttt tggttcgttt cggatggagc cagtgttcat gatcgtttcg caatcagccg
     1921 gaacggctgc gtgcatggcg attgatgcga atgtgccggt gcaacaattg gattacggaa
     1981 agctccggct gcaattgctt gcgaaccagc aggcccttgg aagcagcgtt gtcacgaact
     2041 ccggcatcgt tcaagacaat gccgacccga ctggagtcac gatcacgggc cagtggccgt
     2101 cgagcacttc catcgccggc tattatggca cgaattacat caccgataac aacacgaaca
     2161 agggaacaaa aagcgttcgc ttcacccctg caatcccatc gactggaagc tatcaggttt
     2221 atctatggtg gaccgccgat tacaatcgtg cgaccaacgt tccggtggat gtcatttccg
     2281 cggctggcac gaatacaatc atcattaatc agacgcagaa tggccagcag tggtcttaca
     2341 tcgggacatt taattttacg gcgggaacga gcaacagtgt tttgatccgc acgaccggca
     2401 cgagcggcta cgtcattgcg gatgcggcgc ggttcgtgcc tgcggcgcag gcgtcatccg
     2461 gcgtggacat catttcgacc tgcgggcagg cggcgaaatg gcagaccttg ccgggttggt
     2521 tcactctggt gcgcgcggga agcaccaatt cgccactcac aatcaattac accgtcggcg
     2581 gcaccgcatc caacggcgtg gattatttgc cgctgaataa ctcggtgacg ataccggcag
     2641 gttcggcagc ctcaaccgtc accgtcattc ccatcgcgaa cggattgatc gagggcaatc
     2701 gaaccgtcgt tctcacgttg aacagttcca caaattattc gttcgacgct tatacgaacg
     2761 ccacaataac gattcaggac acgccgctgg cccaatgggc acttgcgcac tttacttccg
     2821 cgcagttgac caatgcggcc atcagcgggg acaacgccga tcccaaccaa gatgggttgc
     2881 cgaacttggg agactatgcc ttggggcttg acccgcttgc cgcaaacaat tcgggcctgc
     2941 cgattttttc gattcaaaat ggattcttca cgcttgctta cttacgcgct gatggcgcgg
     3001 tggatgtgac ttcgagcgtg gaaatgtcaa cgaatctgtt gaattggaat tgcgggtcga
     3061 accatgtgag cgattccgcg attaacgatg atggcaccaa tcagacccgc attgcgcaag
     3121 ccttgcttcc gaccgcatcg cagccgatgt gctttttccg gttcaagttt gcgcggaaac
     3181 aatctcctta aagtttgggg cgatccgcca gcaacggcgc tcgctgcttt gttcaatcaa
     3241 tgtgaatgat gccgcgctgc agggccgttg ttgcagcctg cgtgcggtcg tgaacgcgca
     3301 gtttcgtcag caaatggccg acgtgaagtt tgacggtgac ttcggtaatg gaaagttcgg
     3361 aggcgatttc cttgttgctc tttccggcaa caatcagttc cagcacttcc atctcgcggc
     3421 tggtgaggtc gggatgcgtc atccgctcgg caagccgcgc gccgatggcc gcggggatat
     3481 accgatcccc ggcggcggcg acgcggatcg cgttgatcag ttcctcgcgt gcggcggttt
     3541 tgacgatgta acctttcgct ccggcgtgga acgattgata aatgtcttct tcgccttcgt
     3601 gagtggaaag gatgatgatt gcagcgtcgg ggaattcgtt gcaaatggcc ttggtggcat
     3661 caatgccgct cgccaggggc aagttcaaat ccatcaaaac aagatcgggc tgattttggc
     3721 ggtacatgga aaccgcctgc tcgcccgtgg atgcttccca catcacagcc atatccgatt
     3781 ccaaatcgat cgagccccgc aaacccacgc ggacgacgaa atgatcgtcc acaaccatga
     3841 tttcgatttt tttagacacc cggcgcggca ttacctcgct cacactatca cgaatttgac
     3901 acatcacaat ctctctctgg aatttccagt cctgtttatg gctgaggcaa cgcgttggca
     3961 agccggacat aatccggatc gttcgaagtg gtggcgatgt gcgacagcgg atttgaattg
     4021 ggtgaagacc ggtttgacga gggacaggtt tcatccggcg aattgttggc ggcattgatg
     4081 ccgggtaatt tcgaacccgt ccaagcccat ctctcactat gcccgtccat gaaagaaatg
     4141 atcgcgccgt tgttgtggcg attggagggc gggttccaga attcgggcgt ggccagcgat
     4201 gcgattccaa gcgtgccgtt atcaatgctg aattggtttt cttcaacgaa aacggccact
     4261 tgtgacggct gctgaacgtc gctgtctttt attgcgactg acgtgtaggc atcattcaat
     4321 ccccaagtcg ggcaattgag ctgtgccgag atggaataac ttcgattgtg cggcaccgtg
     4381 ccgttcccaa ccggggcggc cacactcgca tgactggcgg gacatttgta aatgtcggtg
     4441 ttgccgttgt accgccacaa tacgccggtc atgatgttgt tcgtgtaatt cgcgttgcat
     4501 tgttgcacat tgccttggat ccaggcattt ggcccggcgc tgagaccgtt gcctgtatcg
     4561 ttgttcgcca atactccgcc gctgtcgtcg gtgtacatgt aataggcgat ttgcaattgc
     4621 cgttcgttgt tcaagcagct tgtttgccgc gccttcgatt tggcgcgagc cagtgccggc
     4681 agcaacagcc cggcgaggat ggcgatgatg gcaatcacca cgaggagttc gatcaaagtg
     4741 aacccctcgc ggcgatgcca agccctcgcc caaaagccaa attgcatttt ttcgccctcg
     4801 ccgggactgg cggtactgtc gtcatttcga tacgtcaatt tcattgtcct tggattcgat
     4861 agaagcgagt cgttccgggt gtgtctgtgg tggacgtgga agtgctggat gccgtgattt
     4921 gtgagccgag attcgtccag ccggcatcgg tcaggttatt tttatactgc acctgatagt
     4981 tgtgcccgga gacgctttgc cacgtcaaaa gcacgccgcc gccgggttgc gcttgaatcg
     5041 tcagcggaaa agttgccagg ctttgattgg tgttcacttg gaaggcctgg atgccattat
     5101 tggaatccag cacgaacaac tgatttcccg cgaaaatgac cgcgcccgcg ccaggcaagc
     5161 cgttgacggc gttgggattt ttcaccggga acagcttttg atccagcagg acgggatcgg
     5221 cggaaggatt gctgacgtcg tagagccgca catcgtctgg cgtttcaaag gtgagcaccg
     5281 ccagaaggtt ctgcgcggca ttggcgccta acggtccgga attgggcagc acagaattgg
     5341 tggaataggt gtccagcagg gtgcctgtgc cggaattcgg gtcgaattgc accaattgta
     5401 attgctgatt ggctcccttc gcccagaacg tgtttgtgcc agggccgaag gccaggccga
     5461 attccgcgaa agcactgttg ctgacaccgg ccacagtgat gatggtcggg ttaaaaccaa
     5521 aaccgtcggg agtcgtcatc aatgcgacga ttccgatgtt tgttcccgat ccgggcgcga
     5581 gcagaatttg agtgttcacg cccgcgccgc ggacagccat gttgtctccc cacctcatgt
     5641 taggcgccac gggatcgcca ggatcccccg tgtagcaccg ggacggagtc gaattagtgg
     5701 aatcgtcgct ccactgatac aggatgaaat tcgcggtgcc ggcttgtgtt gtcacatttg
     5761 ccgcatagac aactccgtcg tccgcgactc cgatctcatt caagggagac gaaaaggaat
     5821 ccgccgcgcc caggttgccg gtgttcagga aatagcgttg cgcgccggtg gaaggatcga
     5881 gcgccacgat catattagtc gaacccgcgc ggctggccag caacagattg gagctgactg
     5941 gattgtaagc catgccgcgt tcggtcggag agcttccgga accggtgttg gtcagatacg
     6001 ggcgctggcc cggcaaaagc gtccaaacat tgctcatgcg cgacgtattc aaaaccgggc
     6061 tcaccgtgag agtgaccacc gaactggtgg ccccgccgta ggcgttcgta agatgaaggt
     6121 cgtaattccc cgcgctcgcc ggggtgacgt tggtaaacga caacgtggaa ttggttccat
     6181 tgttgttcgt cgcgctggga ttgaccgccg gatttatgtt cacaccattg aactgccatt
     6241 ggggggaaag cggcgcatcg ccgccgatgc cggcggcgat tgtgaggttg gtgtaccctt
     6301 gcagcacgac gaggtttgtc ggagtgggct ggctgctgat ggtcgcggcg aaatggacgc
     6361 cgctgccgcc gctcgagttc gtgaggctga cgttgtcaac gaccagaata tttgtgccgc
     6421 cgctggcgag attgccgcat tcgaaactga tcaaggtcgg gcggtcattg gtgccggacg
     6481 gcgccgcatt ttggaaaaac gtctggttga gaacggcatt gctgccaagg ctgttggtaa
     6541 tgatgacatc gtaattcgga gtcggatccg tgtaacgccc gacaattttg agatggttca
     6601 catgaacggt attcacgtcg ggcatgtaat caatgacgcc cttgagcacg ttgttggccg
     6661 tgctggaacc gctgataagg cgcacggcgt ttgtgatgat gatcaggttg atgttcgccg
     6721 ccgtgccgtt gtggtaaagg ttgagctgaa aagtgcgcga gcttgaattg gtggcgcagg
     6781 cgaaatcaca ttcaaaaata aagttggtca acagtccgcc gggatcggac ggagactggc
     6841 gcaaatagcc ggcgctggta agaacacatt ttgccgaatg gctgccggtg atgatgacgg
     6901 gcgaatcgct cacggtgccg ctggtattgg cccaaccagg gaaaacagtc gtgcccgaac
     6961 cgatgggagc ggcctcaaaa tcaccgttgg ttaggaactg tgcttgtgcg gcggtcgaag
     7021 ccaataaaaa aagcgccgcg caaacggggg cggatttcca aagggtgcgt gatgtggatg
     7081 gtttgttcat gatatatatc ggcgattgtc cgtcgcggct gtttcgttac aggggctatt
     7141 agtgattgac tatagttcgc gcatttcgaa agtcacggaa gcaaaagttg ctgcttggtg
     7201 taataccttg gtataggatg gcattaaggc tattgtccgt ttctcaccct ggatttttcg
     7261 tgcgattgta gaggcatgtc caccatcgct cgcgcgcgca acgccgtccc cggccccttg
     7321 aatccagtct tcgataaatg ttgcaatcgt atttcgcacg gcggaatttt tgggattatc
     7381 atcgccgcgc tgaccatttt ttgcccatgc gcgccggccg aaagcacagg ctcggttttg
     7441 tggggcgtga tggattggaa acttgacgca ttgccgcctg cgacggacac aagcaccacc
     7501 agcccccttt acggcgacgg gaactgccgc atcatttgtc accaatatca aagcgccgac
     7561 tggcttccgt tcaaaatctc cacgaccaac gagatggaaa tcagccgcat ttatggcaag
     7621 ggaagctgca tcaatttgat gctctatttc gtgagttgca cacatgacac gaactcgaac
     7681 acattctcat atccgctcaa cagccaattc ctttccgact tcacgcagct cgtgaacgcc
     7741 tacaaggtcg gcggcggacc gctttacatc caggtgttcc ctgaattcga agaatggtac
     7801 aggacctctt caaccaatga agccaaccaa tatcgggccc aaatcacggc gcagtttttg
     7861 gcaatggccc agatcgccca tacgaattac aaccgcgcat acatcggcct ttgcttcgaa
     7921 ggcggtgatt tcaccggcga cacatcgcag ttcacaaatc gctggaacac gatattgacc
     7981 agcagcgacc tggtgttcgt gaatttcatg tcgccattca cccaggcgaa caccgacgag
     8041 cgcgcgatgg tcctgggaac gcaaatcctg ggcaactggg gtttgccgat gatgtttcct
     8101 tacgtggatt tgtgggatga caacaacacg ccgattttta cggtgggcga caattacacc
     8161 aacgacgcaa cggctttctc gggttacatc accaactgga ttggcagcgc actgacagcg
     8221 cccgccgaag tgcgaggctc gtccagcacg aattggacga ccaatttgcg gacattgaag
     8281 tcgcgcgggc ttttcgcgtt ttccctttat cgcgcttcat ggtgcaataa ttccgtgacg
     8341 ccgcccgacc caaacggcgc gacgcatccg ctgctcgcat acaatatcct caccaacgcc
     8401 atggccacaa acagccgcgc gttcttatat cccatcacgg aaatggaata ttggccttcg
     8461 aaccatccgc ccggcgcgtc cacgacttac gaaattcaaa ccgacacaaa ttgttcatcc
     8521 ggacaattgg ttgttctcga ttcgacgaac gcaacgggac agtattttac ataccgcgcg
     8581 cccgtcacac acaacgggac atatgaagtc tatatcggca tccgcaaaca aaccgatggc
     8641 ggctggttct atttatatgt gaatggttca gtgtcgcagt tgggaagcga acgaaattgt
     8701 tcccttgcca ccaacgtggg caattaccag gaatacgacc agggcgcggt cacgttcagc
     8761 aatctcaccg ggacgacgtg gacggatttc aaattcggcg tcagccgggc
//
LOCUS       DASZMM010000428         4647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1577041, whole genome shotgun sequence.
ACCESSION   DASZMM010000428 DASZMM010000000
VERSION     DASZMM010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577041"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..858)
                     /locus_tag="VG754_10280"
     CDS             complement(97..858)
                     /locus_tag="VG754_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF19647.1"
                     /translation="MQLNFQCHGDGFPLLILHGLLGSLDNWQNISRKLGETFRVCAVD
                     LRNHGRSPHSDAFNYEVMADDLLEFMGTQQIGRAHLLGHSMGGKAAMHFALRHPEKVE
                     KLVVVDMAPRAYAPTHVPIFDAMLSLDTGSFRERGEIDRALATRIPDAATRQFLLKNL
                     TRNEQGALQWKPNLPAIRKNYDGLNEALDTNGSFKGPALFIKGAKSDYITKNDQALIM
                     SMFPNATISEIHQAGHWVHADAPGEFTELVTNFLS"
     gene            complement(888..1289)
                     /locus_tag="VG754_10285"
     CDS             complement(888..1289)
                     /locus_tag="VG754_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19648.1"
                     /translation="MKTSLLLLAFATMFFTGCSSPESVSHMQGRGATEVYDAGFDRVW
                     SAAVAAAQQGDLQILRVDKTHGFICTKRGLGPQSFGENVAIWIRKVKSNQTSVEVVSR
                     QAGPPVLVLRSWEHRILNCISANLTTDTPVS"
     gene            1452..2081
                     /locus_tag="VG754_10290"
     CDS             1452..2081
                     /locus_tag="VG754_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWF19649.1"
                     /translation="MTLAEYTLLAASSLFVIVDPLAVIPAFIAMTVNDAPEQRIRMAR
                     IASFVVAGVLLFFAITGQLIFKILGITMPAFQIAASIVLLLIAIDMLRAQRSRVQQTN
                     EETAAGAEKTDIAVTPLAIPMLAGPGAISTVILLQGQARDIPQRLALYLCIVGVAAVT
                     YLTLRFSARGARWLNPILMNIAIRIMGLLLAAVAVQFMLNAIETLRKSP"
     gene            complement(2084..3121)
                     /locus_tag="VG754_10295"
     CDS             complement(2084..3121)
                     /locus_tag="VG754_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19650.1"
                     /translation="MKKIPAPILIVLSLICVGQFTCQAWDYEGHRLVNQLALTALPTD
                     FPAFVKTPEARERIAFLAGEPDRWRGVREATLDNANAPDHYIDLEQLADYDLDEHSLT
                     HFRNDFTAQLALARAAHPERFKPIDPDQNRDHTRQLVGFLPWAINENFFKLKEGFSYL
                     KAFETGGGTQAEIANARQDIIYIMGVMGHYVGDAAQPLHTTKHFSGWVGDNPQNYTRE
                     HIHSRVDSYFRHFDHASFEAVQARMHPARLPADEESMKSGDTFDAVMAYILEQNKKVV
                     PLYGLEKSGKLFGKGDAGEEGKVFLTQQLQTGGQMLADLWFAAWQQAAPDSYLQSQLA
                     QRKLAEGGTKN"
     gene            3506..4360
                     /locus_tag="VG754_10300"
     CDS             3506..4360
                     /locus_tag="VG754_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWF19651.1"
                     /translation="MTVITDERCIEYLRAGHPERPARISRTLERLRNQKELPITWAAP
                     LPVSETSILRAHTPGHLAALEAGEDFDADTPWFPNIGDHARRSVGGALAALASARAGQ
                     TAFSLMRPPGHHATREVSMGFCYLNSIAIAALEAAASGLKVAVFDFDVHHGNGTEAIL
                     LDQPRTAFFSIHQFPCYPGTGRQNVGGNCFNFPVAPMTPREKYRDTLSKALEGVKKFD
                     LVAVSAGFDAYAQDPLAQETLEAEDYQWLGRSIRELAARNFSVLEGGYSDDLPELVLE
                     YLKGLEGK"
BASE COUNT         1027 a         1327 c         1197 g         1096 t
ORIGIN      
        1 acacaaactg tccaacctgc aaaaatttcc acagttcacc cttcacactg atctgcgcca
       61 atcacctgtt ctcaaatgct gcttgtttac aaaagattaa cttaagaaat tcgttaccaa
      121 ctccgtaaat tctcccggcg cgtccgcatg aacccaatgc cctgcctgat gaatctctga
      181 tatggtcgca ttgggaaaca tgctcatgat caatgcctgg tcgttctttg tgatgtaatc
      241 cgatttggcg cctttgatga acaacgccgg acctttgaaa ctgccgttcg tatcgagcgc
      301 ttcattgagt ccgtcatagt tctttcggat cgccggcaaa ttcggcttcc attgcaacgc
      361 gccctgttcg ttgcgcgtca agttcttgag cagaaactgg cgcgtcgcgg cgtccggtat
      421 tcgcgttgca agcgcgcggt caatttcacc ccgttcacga aacgaacccg tgtccagcga
      481 aagcattgca tcaaaaattg gaacatgcgt cggcgcataa gcccttggcg ccatgtccac
      541 caccaccaat ttttccacct tttcaggatg ccggagcgca aagtgcatgg ccgccttgcc
      601 tcccatcgaa tgccccagca ggtgcgcgcg gccgatttgt tgcgttccca tgaattcgag
      661 caaatcatcc gccatcacct cgtaattgaa cgcatcgctg tgcggcgagc gcccgtggtt
      721 ccgcaagtcc accgcgcaca cgcgaaacgt ttcacccagc ttgcggctga tgttctgcca
      781 gttgtcgagc gaccccaaca acccgtgcaa gatgagcagc ggaaaaccgt cgccatggca
      841 ttggaaattc aactgcatag gcccgtttca taaacccaga ctctccttta tgaaaccggc
      901 gtgtcagttg tcaagttcgc tgaaatgcaa ttgagaatgc gatgttccca actgcgcaac
      961 accagcaccg gcggtccagc ctggcggctc accacttcaa ctgacgtttg gttcgacttc
     1021 actttgcgga tccaaatggc cacgttttcg ccgaaacttt gcggcccaag cccgcgcttg
     1081 gtgcagatga aaccatgggt cttgtcaaca cgcaggattt gaagatcacc ctgttgcgcc
     1141 gctgcaacag cagcgctcca cacgcgatca aagccggcat cgtaaacctc tgtcgctccg
     1201 cggccctgca tgtggcttac cgattccgga ctggaacatc ctgtgaagaa cattgtcgcg
     1261 aacgcaagaa gaagcaaact cgttttcatt tttttaatcg ccagaacttt tttgatttcg
     1321 ccgataattt aattcaattg cgttctgcgc caatggggtg ttcacctctc aactctcaat
     1381 ttgcggataa ataatcgcgc gggttttcaa ctttcaactt tgaacgcgga actttaaact
     1441 tggcggcaac gatgaccttg gccgaataca cgctgcttgc cgcgagttca ctgttcgtga
     1501 ttgtggaccc cctggcggtg attccggcgt tcatcgcgat gacagtcaac gatgcgccgg
     1561 aacaacgcat tcgcatggcg cgcatagcgt catttgtggt cgcgggcgta ttgctttttt
     1621 tcgcgatcac gggacaattg attttcaaga tccttggcat cacgatgccc gcgtttcaaa
     1681 tcgccgcaag catcgtgctg ctgcttatcg cgattgacat gctccgcgcg caacgctccc
     1741 gcgtccaaca aaccaatgaa gaaaccgccg caggcgcgga aaaaaccgac atcgccgtga
     1801 cgccgctcgc cattccgatg ctggccgggc cgggcgcaat ctcaaccgtg attctcctgc
     1861 aagggcaggc gcgggacatt cctcaacggc tggctttgta tctctgcatc gtcggtgtcg
     1921 cggcggtgac gtatttgacc ttgcgcttct cagctcgcgg cgcgcgctgg ctcaatccca
     1981 tcctcatgaa tatcgccatc cgcatcatgg gcctgctgct ggccgccgtg gcggttcaat
     2041 tcatgctcaa tgcgattgaa acgctgcgga aaagtccgtg agtttaattc ttggtcccgc
     2101 cttcggccaa tttacgttgg gcgagctggc tttgaagata actgtccggg gcggcctgtt
     2161 gccacgcggc gaaccaaagg tccgcaagca tctgaccgcc cgtttgaagc tgctgggtga
     2221 ggaacacctt gccttcttcg cctgcgtcac ccttgccgaa cagtttgccc gatttttcca
     2281 atccatacag cggcaccacc tttttattct gctcaaggat gtatgccatc acggcgtcaa
     2341 atgtatcgcc tgatttcatg ctctcctcgt ccgccggcaa gcgcgccgga tgcatcctcg
     2401 cctgtaccgc ttcaaaagat gcatgatcaa aatgccggaa ataactgtcc acgcggctgt
     2461 ggatgtgctc gcgcgtgtaa ttctgcggat tgtcccccac ccagccgctg aaatgcttgg
     2521 tcgtgtgcag cggttgcgcg gcgtcgccga cataatgccc catcacgccc atgatgtaga
     2581 tgatgtcctg ccgcgcgttt gcgatttcgg cctgtgtgcc tccgcccgtt tcaaaagctt
     2641 tgagataaga aaagccttcc ttgagcttga aaaaattctc attgatggcc cacggcaaaa
     2701 aaccgaccaa ctggcgcgtg tggtcgcggt tttggtccgg atcaatcggc ttgaaccgtt
     2761 ccggatgggc cgcgcgcgca agggcaagtt gcgcggtgaa atcgttcctg aaatgggtca
     2821 gcgaatgttc gtccaagtca taatcggcga gttgttcgag gtcgatgtaa tggtcgggcg
     2881 cattcgcgtt gtcgagcgtc gcttccctga caccgcgcca acggtcgggc tcgcccgcaa
     2941 gaaaagcgat gcgttcacgc gcttcgggtg tcttgacgaa ggccgggaaa tcagttggca
     3001 gcgcggtcaa cgccagttga ttcacaagac gatggccttc atagtcccaa gcctggcagg
     3061 tgaactgtcc aacgcaaatg agggaaagca ctataaggat cggggccggt atttttttca
     3121 tggtctgctc tttaattttg caccactgtt actgagcgac ataaaaaagg caacagatga
     3181 aacggcgaga agctttcaat gggcacatcc ggccactgct gccgaagtgg ttacccggcg
     3241 gccgaaatgt agcgcagagt tgcactctgc tgtatcgcgg agtttcactc cgcaagcccg
     3301 cgcggtggca ccgcgatttt agcaattcag agcttccggg tgtttttttg atggtccagc
     3361 cgactgcaag tcggcgatac agcagattaa aaatctgcgc tacaagggct ggcgtcaatc
     3421 tgtcacaagc ttctgaagtt gcagggcaat atcaagaacc gcccacccag aattttccaa
     3481 cactccatca ctccatcgca ttcgcatgac agtcatcacc gacgaacgct gcatcgaata
     3541 cctccgcgcg ggacatcccg aacggccggc gcgcatcagc cgcaccttgg aacgattgcg
     3601 caaccaaaag gaattgccca tcacgtgggc cgcgcccctg cccgtttccg aaacttccat
     3661 tctgcgcgcc catactccgg ggcatcttgc ggctttggaa gcaggcgaag atttcgacgc
     3721 cgacacgcct tggtttccga atattggcga ccacgcgcgt cgctcggttg gtggagcgct
     3781 tgcggccctt gcctcggcgc gcgcggggca aacagccttc agcctcatgc gtccacccgg
     3841 acatcacgca acccgcgaag tgagcatggg attttgttat ctcaattcga ttgccatcgc
     3901 ggcattggaa gcggcggcaa gcggattgaa ggtcgccgtg tttgattttg acgtgcacca
     3961 cggcaatggc acggaagcca ttttattgga ccaaccgcgc accgccttct tctcaatcca
     4021 tcaatttccc tgttatcccg gcaccggaag acaaaacgta ggcggcaatt gtttcaattt
     4081 tccggtcgcg cccatgacgc cgcgcgagaa atatcgcgac accctttcga aggcgctcga
     4141 aggcgtgaaa aaattcgatt tggtcgccgt gtctgcggga ttcgatgctt acgcgcaaga
     4201 cccgctcgcg caggaaacgc ttgaagcgga ggattatcag tggcttggcc gcagcatccg
     4261 cgaacttgcc gcgcgtaatt tcagtgtgct cgaaggcggt tacagcgacg atctgccgga
     4321 acttgtcctt gagtatttga agggcttgga aggcaaatag aagatctttt tcagagctaa
     4381 ccgttggaag atatgattag gaaagcagga aatcaggaaa gacgatgttt cctcaagaat
     4441 tttgttcctg atttcatgat ttcctaataa aaaactctta atctcatttc gacttggccc
     4501 aacaaaaact ccgaaatgtc ttgccttcaa aaaggcctgt gatagtctcg tttccgttgg
     4561 aaggaggcgg gccagttctt tgacatccta aaatgattaa tggctgagag ttgagcgttg
     4621 agtaaatgag actcaagccg aaatcga
//
LOCUS       DASZMM010000429         5801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1823308, whole genome shotgun sequence.
ACCESSION   DASZMM010000429 DASZMM010000000
VERSION     DASZMM010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823308"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VG754_10305"
     CDS             <1..1131
                     /locus_tag="VG754_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF19652.1"
                     /translation="RAGVDVLVLEKHKDFFRDFRGDTIHPSTLELMYELGLLEEFLAQ
                     KHQELCEITAHFGPKVIHIADLTHVPAHCKFIAFMPQWDFLNFLCKHAQKYPGFQVRM
                     ESDVTDLLWENERVAGVKVRTPKGTVEVRAQLVVGADGRSSTVRQCAGLEIEDLGAPI
                     DVLWFRISKKASDPPQAFGFVNAGQFVVLIDRADYWQCAYVIRKGSFEQKRERGIQAF
                     REDIARCVPFLEDRVGEIKEWDDVKLLSVKVDHLRKWYREGLLCIGDSAHAMSPVGGV
                     GINLAIQDAVATANLLGPKLLKKKLQMEDLEAVQARRERAARLTQRLQIFLHKHMLEK
                     IFDSRVMISPPFFLALVEHFPRLRRVTARMIGIGFRPEHINK"
     gene            1561..2901
                     /locus_tag="VG754_10310"
     CDS             1561..2901
                     /locus_tag="VG754_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWF19653.1"
                     /translation="MNAPTLPPFDYKPKPYTGPSAEEVLRLRKEFLSPALFLYYKKPV
                     MFVEGKGQYIFDDKGRRYLDGLGGIVTVSVGHCHPYVLEAVRKQNETLQHSTTIYLHP
                     NIAEFGQKLASKMPGDLKVCYFVNSGSEANDLALLMARAYTGNYDVIALRNSYHGGNA
                     SGMSMTSHSTWKFNVPHSFGVHHAICPDPYRGAYGRNDPEAGKKYAADVKQLVDFATS
                     GQIAGFIAESIQGVGGVVVFPDGYLKHAYEHVRAAGGVCIADEVQAGFARTGTHYWGF
                     ETQGVVPDIVTMAKGIGNGCPLAAVVTTPQIASVLAKRIHFNTFGGNPVVCAQGKAVL
                     EVIDNEKLQANALTIGNYIMTGLERLKAKHNVIGDVRGKGLMLGIELVKDRATKEPAK
                     SECAQVLETCKDLGLLLGKGGLSGSVIRFAPPMCVTKADADFMLEVFDAAFGTL"
     gene            complement(3026..3520)
                     /locus_tag="VG754_10315"
     CDS             complement(3026..3520)
                     /locus_tag="VG754_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWF19654.1"
                     /translation="MTTVAEQLQRAREVQSLSINQVAEVTKIRTDHIRAIEEGNFDIF
                     AAPVYIRGFVRTYALMLKLDVGDIMSALDKELGKTEKFREPPPLSEHKRGILDFLTLQ
                     LSKLNWRIAFVGVAGLVVVGIIYFCVSLVRHHHRADPLSNLTPGTYQPSTSGETVPLP
                     PTHR"
     gene            complement(3684..4577)
                     /locus_tag="VG754_10320"
     CDS             complement(3684..4577)
                     /locus_tag="VG754_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF19655.1"
                     /translation="MKRFMVLLPLFFINIFANAQTGSNAVIVLWPDGAPGALGKTDKD
                     IPTLTVFLPEPEKATGAAMVVLPGGGYGGLAQHEGRDYALWLNANGVTCFVLKYRLGS
                     SGYRHPIMLQDAARALRTVRARAAEWKLDTKRIGIIGSSAGGHLASTLLTHFDAGNPD
                     APDPIDRQSSRPDLGVLCYPVITMGDKTHLGSRNNLLGKDPSPELIKELSNELHVTKE
                     TPPCFLFHTFEDNAVPVENSLMFAEALRRAGVPFDLHIYQSGKHGSGLGGVPYSTYDP
                     EKLHPWTRDCIFWLKQQGFVK"
     gene            complement(4631..5749)
                     /locus_tag="VG754_10325"
     CDS             complement(4631..5749)
                     /locus_tag="VG754_10325"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HWF19656.1"
                     /translation="MNSTSLNWILIFAALLPWVAIAVGMLMTPRRTRPDLFFAVTVNP
                     SFQTSPLGNEIVRQYNRLVIIVALVALLPIGLIPLSPRLMLVGLIAPTLIQIAGGLGA
                     LLMARHRTLPHRVQPTSEREADLTPRHASLAGGWLAQAGPFLILAVFAICLWLNWDRV
                     PARFPIHWGLDGKPNGWGVKSFGSVFGIWITSVFLCLFICGIQYMTVYGAVTARINNV
                     RDGMPQARFVRAISWFLLGVEYWLALLVGLLSLLPLRSSQQLPLPIIWPILLGQTLII
                     ATIFIIAYRMGQGGWRLQTEGEKINADACPIGDRTPDECWKLGVFYFNRTDPALFVEK
                     RFGVGWTLNMARPGAWAFLGAILLLSVGSVVIGICASR"
BASE COUNT         1306 a         1635 c         1647 g         1213 t
ORIGIN      
        1 cgcgcgggtg tggacgtcct cgtgttggaa aagcacaagg attttttccg cgattttcgc
       61 ggcgatacca ttcatccctc gacgttggag ttgatgtacg aactcgggtt gctggaagaa
      121 tttctcgccc aaaagcacca ggagttgtgc gagatcactg cccattttgg acccaaggtc
      181 atccatatcg ccgatctcac gcatgtgccg gcgcattgca aattcatcgc cttcatgccg
      241 caatgggatt ttttgaattt tctttgcaaa cacgcgcaaa aatatccggg ctttcaagtc
      301 cgcatggaat cagacgtgac ggatttgttg tgggaaaacg aacgggtcgc gggcgtgaaa
      361 gtccgcacgc cgaaaggtac ggtcgaagtg cgcgcgcaat tggtggtcgg cgcggacgga
      421 cggagttcca ccgtgcgcca atgcgcggga ctcgaaattg aagatctcgg cgcgccgatt
      481 gacgtgcttt ggtttcgcat ctcaaaaaag gcgagtgatc cgccgcaggc ttttggtttc
      541 gtgaacgccg gacagttcgt ggtcctgata gatcgcgccg attactggca atgcgcttat
      601 gtgatccgca aaggctcctt cgagcaaaag cgggagcgcg gcattcaagc gttccgcgag
      661 gacattgcgc gatgcgtccc gttcctggaa gaccgcgtcg gcgaaatcaa agaatgggat
      721 gacgtcaaac tcctgagcgt gaaggtggac catttgcgaa aatggtatcg cgagggcctt
      781 ctttgcatcg gcgattctgc tcacgcgatg tcgcccgtcg gcggcgtcgg catcaatctc
      841 gccatccagg acgctgtcgc gacggcaaat ctccttggtc caaaattgct gaagaagaaa
      901 ttgcagatgg aagatttgga agcggtgcaa gcccggcgtg aacgtgcggc ccggcttacg
      961 caacgtctcc aaatttttct gcacaaacat atgctcgaga aaatttttga ttcgcgcgtg
     1021 atgatttctc ctccgttctt cctggccctt gtcgaacatt ttccgcgcct gcggcgagtc
     1081 actgcgcgaa tgatcggcat cggcttccgg cctgagcata taaataagta gtgagtcgct
     1141 gactgaatcg tggagcgcgg ctcgatgagc cgcagcaatg catggcaacc aagacacgcc
     1201 cgactgattc atatgcgccc aaacttccca cattgctgcg ggtcgccgac cccgcgctcc
     1261 ggggtgctgc gcggatgcat cctggcatgg cgcgcatctt tgacactgtt gcctagcggg
     1321 gactggaaag tccccgcaac ccgcaggttg gaaagcctgc gccacgtagc gcagacattc
     1381 ttgtctgccg gttcaggcga ctttccagtc gcctgaattc ctgcatccaa attcctctat
     1441 caaaaacagt tccaagccgc tgcggttggt cctgcggaca tagacgcgct tgacttggtt
     1501 cttgccgatg gcgcacgatg gggaactgat atataatttc cgaatcaaca aaggccaaac
     1561 atgaatgcac cgactttgcc tcccttcgac tacaaaccca agccctacac cggtccttcc
     1621 gccgaggaag tgctgcgctt gcgcaaggaa tttttaagcc ccgcgctgtt tctttattac
     1681 aaaaagccgg tcatgttcgt cgagggcaag gggcaataca ttttcgacga caaagggcgg
     1741 cgttatctgg atggattggg cggcatcgtg acggtgagcg tcgggcattg ccatccgtac
     1801 gtcctggagg cggtgcgcaa gcagaacgaa accctccagc attccacgac gatttatctg
     1861 catccgaaca tcgcggagtt cggccaaaag ctcgcctcga aaatgccggg cgacctcaag
     1921 gtctgttatt tcgtcaattc cggttccgag gcgaacgacc tcgcgctgct catggcgcgc
     1981 gcttacaccg ggaattacga tgtcatcgcg ctccgcaatt cctatcacgg cggcaacgct
     2041 tccggcatgt cgatgacgtc gcacagcacg tggaaattca atgtgccgca cagcttcggc
     2101 gtgcatcacg cgatttgccc cgacccatat cgcggcgctt atggccgcaa cgatcccgaa
     2161 gccggcaaaa aatacgcggc ggacgtgaag caactggttg atttcgcaac gtccggacaa
     2221 atcgccgggt tcatcgcgga atccatccag ggcgttggcg gcgtcgtggt gttcccggac
     2281 ggttatctca agcacgctta cgaacacgtc cgcgctgccg gcggcgtctg catcgcggac
     2341 gaagtgcagg ccggtttcgc gcgcacgggc acgcattatt ggggctttga aacgcagggc
     2401 gtggttcccg acatcgtcac gatggccaaa ggcatcggca atggttgtcc gctcgcggca
     2461 gttgtgacga cgccgcaaat cgcgagcgtt ttggcgaagc ggatccattt caatactttc
     2521 ggcggcaatc ccgtcgtttg cgcgcaaggc aaggcggtgc tcgaagtgat cgacaacgaa
     2581 aaactgcagg cgaacgcgtt gaccatcggc aattacatca tgaccgggct tgagcgattg
     2641 aaggccaagc acaacgtcat cggcgatgtg cgcggaaaag gtttgatgct cggcatcgaa
     2701 ctcgtaaaag accgcgcgac gaaagaaccg gcgaagtccg aatgcgcgca agttttggag
     2761 acgtgcaaag acctgggttt gctgctcggc aaaggcggac tttcgggaag cgtcatccgc
     2821 ttcgccccgc ccatgtgcgt gaccaaggcc gacgcggatt tcatgcttga agtgttcgac
     2881 gcggcgtttg ggacgttgtg acggatgcaa atccgaaacg ctgaacaacc aacatcgaac
     2941 attgaacgct gaatgttcgg agttggatgt tcgatgtttt ccccgctgtt cttcaaaaga
     3001 gatggagaat ccagcgcgac ccggatcacc gatgcgtcgg cggcagcgga accgtttcgc
     3061 cggaagtgga gggctgatac gtgccgggag ttagattgct gagcgggtcc gccctgtgat
     3121 gatgccgcac aagcgatacg caaaaataga tgatgcccac cacgacaagc cccgccactc
     3181 caacgaatgc gatgcgccag ttgagcttgg aaagttggag cgtgagaaaa tccaagatgc
     3241 cccgcttgtg ctcagagagc ggcggcggct cgcggaattt ttccgttttg ccgagttcct
     3301 tgtcgagcgc gctcatgatg tcgccgacgt cgagcttcaa catcaacgcg taagtccgca
     3361 caaaaccgcg gatgtaaacc ggcgccgcga agatatcgaa gtttccttcc tcaatggcgc
     3421 ggatgtggtc cgtgcggatc ttcgtgacct cagcgacctg gttgatcgag aggctttgga
     3481 cctcccgcgc gcgctgaagt tgttcggcaa ccgttgtcat aacggctctt ttaacggttt
     3541 ttcgatggtt ttggcaaccc tgtaaatcag cctttcaaca tgattgcatc cggcagataa
     3601 tggtgattca gggaaacttt ggtttctgag ttgcggccat gctaaatcgt tgcattgcga
     3661 atttcccatt ttttccattg gagttacttc acaaaaccct gttgcttgag ccagaagatg
     3721 cagtcacgcg tccagggatg gagcttttcc ggatcgtaag tgctgtacgg cacgccgcca
     3781 agtccgctgc cgtgcttgcc ggattgataa atgtggaggt caaacggcac gcccgcgcgg
     3841 cgcaacgcct cggcaaacat gaggctgttc tccaccggca cggcgttgtc ctcgaacgta
     3901 tgaaacaaaa aacatggcgg cgtttctttc gtgacgtgca gctcgttcga aagttccttg
     3961 atcaattccg gcgacgggtc cttgccgagc agattattac gcgaacccaa gtgcgttttg
     4021 tcgcccatcg tgatgacggg ataacacagc acgccaagat cgggccggga actttggcga
     4081 tctattgggt cgggcgcgtc gggattgccg gcatcgaaat gcgtgagcaa ggtcgaggca
     4141 agatgcccgc ccgccgagga gccgatgatg ccgatgcgtt tggtgtccag cttccattcc
     4201 gccgcgcggg cgcgcacagt tcgaagggcc cgcgccgcgt cctgcaacat gattggatgc
     4261 cggtaaccgg acgagccgag acgatatttc aaaacgaaac atgtgactcc gttcgcgttc
     4321 aaccagagcg cgtaatcgcg tccttcatgt tgcgcaagcc cgccgtaacc gccgccggga
     4381 agcaccacca tcgccgcgcc ggtcgccttt tccggctcgg gcaaaaacac ggtcagcgtc
     4441 gggatgtcct tgtccgtctt gcccaatgcg cccggcgcgc catcgggcca gagcacgatg
     4501 accgcattgg aaccggtctg ggcattggcg aaaatgttga tgaagaaaag cggcaacagg
     4561 accataaagc gtttcatagt gtgccgggat tatatccaga gcatctcgcc gaggcaaatc
     4621 attcaatatt tcagcgtgac gcgcagattc caatgaccac gcttcccacg cttaaaagca
     4681 ggatcgctcc caaaaacgcc cacgctcctg gtctggccat gttcaacgtc caacccacgc
     4741 cgaagcgttt ttcgacgaac aacgccgggt cagtgcgatt gaaataaaac acgccgagtt
     4801 tccagcactc gtcgggcgtc ctgtcaccga tcgggcatgc atcggcgttg attttttcgc
     4861 cttctgtttg caaccgccag ccgccctgcc ccatgcgata ggcgatgatg aagatggtcg
     4921 caataataag cgtttgcccc agtagaatcg gccaaataat gggcagcggt aattgctggg
     4981 atgagcgtag gggcagcaaa ctcaacaatc ccaccagcag agcgagccaa tattccacac
     5041 cgagcagaaa ccatgaaatc gcccggacaa accgcgcttg gggcattcca tcgcgtacgt
     5101 tgttgatccg cgctgtaacc gcgccatata ccgtcatgta ttgaatgccg caaatgaaga
     5161 ggcaaagaaa cacactggtg atccagatgc caaacaccga gccgaagctc ttgacgcccc
     5221 agccgttggg tttgccgtcc agcccccaat gaatcggaaa gcgcgccgga actctgtccc
     5281 aattcagcca gagacaaatg gcaaacaccg cgaggatcaa aaacggaccc gcttgggcca
     5341 gccaaccgcc ggcaagcgag gcgtgccttg gcgtcaagtc cgcctcgcgt tccgaggtcg
     5401 gctggacacg atgtggaagc gtgcgatgcc gggccataag aagcgcgcca aggccgcctg
     5461 caatttgaat caatgttggc gcaatcagtc caaccagcat cagtcgcggc gacaacggaa
     5521 tcaaccctat cgggagcaac gcaaccagcg ccacgatgat gaccagccgg ttatactgac
     5581 gcacaatttc gttcccaagg ggtgaagttt gaaacgatgg attgaccgtg acggcgaaga
     5641 acaagtcggg ccgcgtccgg cggggagtca tcagcattcc cacggcgatg gccacccatg
     5701 gcaacagcgc cgcaaaaatc aatatccagt tcaagctcgt tgagttcata cgttcgctcc
     5761 ttctatttct tcaattcatc cgacaaggca ttcagttgag a
//
LOCUS       DASZMM010000430         4649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2100258, whole genome shotgun sequence.
ACCESSION   DASZMM010000430 DASZMM010000000
VERSION     DASZMM010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100258"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2353
                     /locus_tag="VG754_10330"
     CDS             <1..2353
                     /locus_tag="VG754_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWF19657.1"
                     /translation="AREGARIAFGEVAEAHCHFEKAKVVVALDSNFLLEHPQRLRYAR
                     EFTNGRRLAAGAKEMNRLYVAESTPTITGATADHRLPVSSSDIAKIASDLLHQLEGNG
                     NATAKSPADVRPRWIAAVAAELQKNRGAGIVIAGEQQPPLVHAIVHRLNDILGNAGQT
                     VTCTSSAEAQPVNQLSSLRELARDMAAGRVELLVMLGGNPVFTAPADFAFATQLARVK
                     TCVHVSLEEDETSAHCHWHIPRTHFLEEWSDTRSFDGTVSIAQPLIAPLYENRSALEV
                     MAVLNEQLQQQAIPGSYEIVREYWQQQKLGPDFEKAWRKAVHDGLMANTASRPKQVRL
                     NTQEIDSQIARLNNSAAETLEISFHPDPSIWDGRFANNGWLQELAKPISKLTWDNAAL
                     ISPALAQKRQLSTGDVVELRFKGRKVRAPVWIMPGQAENSVALHLGQGRTRTGRVGNG
                     VGFDAYQLRVSDALWFGSGLEIAKTGERHQLVTTQLQHVIEGRDNLQDRGILFTGTLA
                     QFRDDPKFLDKEIKQADAETPPRDETLYDPKEHDYAGYRWGMAIDLTTCIGCNACIMA
                     CQSENNIPVVGKDQVQKGRDMLWIRVDSYYSGGMDNPEINQMPVPCMHCENAPCELVC
                     PVAATLHDHEGLNLMVYNRCVGTRYCSNNCPYKVRRFNFLQYADYHTPSLKPMRNPNV
                     TVRWRGVMEKCTYCIQRISAARITADEQNRRIRDGEVVTACAQACPADAIVFGDLSDP
                     NSKVSRIKAEPLNYNMLGELNTRPRTTYIAKVRNPNPELEKPA"
     gene            2366..3760
                     /gene="nrfD"
                     /locus_tag="VG754_10335"
                     /pseudo
     CDS             2366..3760
                     /gene="nrfD"
                     /locus_tag="VG754_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
     gene            3778..4317
                     /locus_tag="VG754_10340"
     CDS             3778..4317
                     /locus_tag="VG754_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HWF19658.1"
                     /translation="MNGEDALYGLMAEFEHEEEVVAAARRAYEAGYRKMDAYTPFPVE
                     GLAEELGSHGTWVPAIVLTGGIIGGLSGYFMEWYATVIDYPLNIGGRPFHSWPAYIPI
                     TFELTVLTASLCALVGMLALNRLPEPYHPVFNAPGFEHATQDKFFLCIESTDPKFDKE
                     AVRNFLESLGPRRISEVAR"
     gene            4335..>4649
                     /locus_tag="VG754_10345"
     CDS             4335..>4649
                     /locus_tag="VG754_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF19659.1"
                     /translation="MIVTLLLLACGCRPDMFNQPRNNPLSSSDFFENGAGSRPLVPGT
                     IARGHLEEDREFYTGMSGTNLVTEFPLPVNRAVLERGRERYDIYCSVCHGLSGDGDGM
                     IVQ"
BASE COUNT          981 a         1185 c         1401 g         1082 t
ORIGIN      
        1 tgcgcgcgaa ggcgcgcgga tcgcattcgg cgaagtggcc gaagcgcatt gccatttcga
       61 gaaggcgaag gtggtggttg cgctcgattc aaatttcctg ctggagcatc cgcaacggct
      121 ccgttacgcc cgcgaattca caaatggccg caggttggct gcgggcgcga aggagatgaa
      181 ccggctttat gttgccgaaa gcacgccgac catcacgggc gcgacggcgg atcatcgttt
      241 gccggtgagt tcgagcgaca ttgccaagat tgcaagcgac ttgttgcacc agcttgaagg
      301 aaacggcaac gctacggcga aatcgccggc tgatgtgcgg cctcgttgga ttgcggcggt
      361 cgcagccgag ttgcagaaaa atcgcggcgc gggaatcgtc attgcggggg agcaacaacc
      421 gccgctcgta catgcgattg ttcatcggct gaatgacatc cttggcaacg caggccaaac
      481 cgtgacctgc acgtcgtccg ccgaagcaca gccggtcaac caactcagct cgttgcgtga
      541 attggcgcgg gacatggctg cgggacgtgt cgagttgctg gtgatgctcg gcggcaatcc
      601 ggtattcacg gcgcccgccg atttcgcatt cgcaacacaa cttgccaggg tgaagacctg
      661 tgtccacgtg agtttggagg aggacgaaac ttcagcgcat tgtcattggc acattccccg
      721 gacacatttt ttggaggaat ggagcgatac gcgcagtttt gatgggacgg tttccatcgc
      781 gcagcccttg attgcgcctt tgtacgaaaa tcgttcggct cttgaagtca tggctgtgct
      841 gaacgagcaa cttcagcaac aggcaattcc gggcagttac gaaatcgtgc gcgaatattg
      901 gcagcagcaa aagttgggac ccgatttcga aaaggcttgg cgcaaggcag tgcatgacgg
      961 cctgatggcc aacacggcgt cgaggcccaa acaggtgcgg ttgaacacgc aagaaataga
     1021 ttcgcaaatt gctcgactca acaatagtgc cgcggagact ttggaaattt cctttcatcc
     1081 cgatccttcg atttgggacg gacgatttgc gaacaacggc tggttgcagg aattggccaa
     1141 gccaatttca aaattgacct gggacaatgc cgcgttgatc agcccggctc tcgcgcagaa
     1201 gcggcaatta tccacgggcg acgtggtcga gcttcggttc aaagggcgca aagtgcgcgc
     1261 gccggtttgg atcatgccgg ggcaggcgga gaattctgtg gcattgcatt tggggcaagg
     1321 acggacacgc accggacgcg tcggcaacgg tgtgggtttc gatgcctatc aattgcgcgt
     1381 ttccgatgcg ctttggtttg gttcgggatt ggagattgcg aagaccggag aacggcatca
     1441 actcgtcacg acgcaattgc agcatgtgat tgaaggcagg gacaatctgc aagaccgggg
     1501 catcttgttc accggcacac tggcgcaatt ccgcgatgac ccaaaatttc tggacaaaga
     1561 aatcaagcaa gccgatgcag aaacaccgcc ccgtgatgaa actctctacg atcccaaaga
     1621 gcacgattac gcagggtatc gctggggcat ggcgatcgat ctgacgactt gcatcggttg
     1681 caacgcgtgc atcatggctt gccagtcgga gaacaacatt cccgtcgtcg gcaaagacca
     1741 agtccagaag ggacgcgaca tgctttggat acgcgtggac tcgtattaca gcggagggat
     1801 ggacaatccg gaaatcaacc agatgccagt gccgtgcatg cattgcgaaa atgcgccgtg
     1861 cgaattggtc tgcccggtcg ccgcgacatt gcacgatcac gaaggcttga acctcatggt
     1921 ctataaccgt tgtgtcggca cgcgttattg ttccaacaac tgtccttaca aggtgcggcg
     1981 attcaatttt ctgcaatacg ccgactatca cacgcccagc ctcaagccga tgcgcaaccc
     2041 gaacgtgacc gttcgctggc gcggcgtgat ggagaaatgc acttattgca tccaacgcat
     2101 cagcgcggcg cgcatcaccg ccgacgaaca aaatcgccgg attcgcgatg gcgaagttgt
     2161 caccgcttgt gcgcaggcgt gcccggcgga tgccattgtg ttcggcgatt tgagcgatcc
     2221 gaacagcaaa gtgtctcgca tcaaggcgga accgctgaat tacaacatgc tgggcgagtt
     2281 gaacacgcgc ccgcgcacca cgtatatcgc gaaggtgcga aatccaaatc cggaattgga
     2341 aaaaccggcc tgagtcaacc accctatgcc tgagaacaat ccagcgccgg tgaatgcccg
     2401 caatgaccgc ggttgcgacg aactttcgcc gctcggttat cccacgcaaa ttctttgtcc
     2461 ggggcagacg tacgaaaccg tcaatgaaac catcggttca attgttcttc gtccgaggcg
     2521 aaccagattt tggatcaccg gcatctttat ttcttttttg atgttgatga tgtttttgtg
     2581 ggcggtgacc tacctgcttt tgaagggggt gggcatttgg ggcatcaata ttccggttgc
     2641 gtggggattt gccatcgtga atttcgtctg gtggattggg attggccatg ccggcacgtt
     2701 catctcagcc atcctgctgc tgcttctgca aaaatggcgc acggccatca atcgcctgac
     2761 cgaggccatg accttgttcg cggtgatgtg cgccgggctg ttcccgctgc tgcatttggg
     2821 caggccatgg gttttcttct ggctggttcc ctatcccgac acgatgaaga tgtggccgca
     2881 gttccgcagt ccgctggtct gggacgtgtt cgcggtcagc acttatttca cagtgtcgct
     2941 gattttttgg tacatcggtt tgataccgga tgtcgccaca ttgcgagaca gggcaacgac
     3001 gcgcggcaaa caactttttt acggtgtgct ggcgtttgga tggcgcggtt cggcgcgtca
     3061 ttggcaacga catcaagcgg cttatttgct gttggcgggc cttgcgactc cgctggtgct
     3121 ttcggtccac agcgtggtga gcctggattt caccgtggca atcgtgccgg gctggcactc
     3181 gacgattttt ccgccgtact ttgtcgcggg cgcgatttat tccggatttg cgatggtgtt
     3241 gaacatcctg attccgctgc gaaaattata cggattggag gagatcattc cgctgcgtta
     3301 catgaacaac atggccaaca tcatgctcgt gactggttgg atggtgacgt tcggatacat
     3361 catggaggcg ttcatggcct attacagcgg ggatgttttt gagcgataca tgatgtggaa
     3421 ccgcgcgttt ggtccttacg gttggatttt ctggctcttg atgctcacca acgtgttcat
     3481 cccgcaagtg ctttggtcga ggcgcgtgcg gacgaacatg ctttcgttgt tcctggttgc
     3541 gctctcggtg aacgtcggca tgtggttgga acggttcgtg atcgttgtta caagcctcaa
     3601 ccgcgatttc acgccgtcca tctggcgcat gttttacccg accaagtggg actggatgac
     3661 gttgttcggc agcatgggtt tgtttttgtt tctcctgttt ttgttcatcc gtttcctgcc
     3721 gttgatttca atttcagaag tgcgcgaact ggtcgccgag gaacgaaagg gagaagcatg
     3781 aacggcgaag acgcgcttta cgggctgatg gcggaattcg agcacgaaga agaagtggtc
     3841 gcggcggcac ggcgcgctta cgaggcgggc tacaggaaaa tggacgccta cacaccgttt
     3901 ccggtcgagg gattggcgga agaattggga agtcatggaa cgtgggtgcc tgcgattgtg
     3961 ctcacgggcg gaatcattgg cggcttgagc ggatacttca tggaatggta cgcgactgtg
     4021 attgattatc cgttgaatat cggcggaaga ccttttcata gctggccggc gtatattcca
     4081 atcacattcg agttgacggt gctgacggct tccctctgcg cgttggtggg aatgctcgcg
     4141 ttgaaccggc tgccggaacc ttatcatccc gtcttcaacg cgcccggttt cgagcacgcg
     4201 acgcaagaca agttttttct ttgcatagaa tcgacggatc caaagttcga caaggaggcg
     4261 gtgaggaatt ttctggagag cctggggccg cgaaggattt cggaggtggc gagatgaaat
     4321 gggtggttcg aatcgtgatt gtcacactgc ttttgctcgc gtgcggctgt cgtcctgaca
     4381 tgttcaatca gccgcgaaac aatccgctga gttcgagcga tttttttgaa aatggagcag
     4441 gctcacgtcc gttggtgccc ggcacgattg cgcgcggtca tctggaggaa gaccgggagt
     4501 tttatactgg catgagcggc acaaatctcg tcactgaatt tccattgcca gtcaaccgcg
     4561 ccgtgctcga acgcgggcgc gaacgttacg atatttattg ttcagtctgc cacggtctca
     4621 gcggagacgg agacgggatg attgtgcag
//
LOCUS       DASZMM010000431         5256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2652818, whole genome shotgun sequence.
ACCESSION   DASZMM010000431 DASZMM010000000
VERSION     DASZMM010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652818"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VG754_10350"
     CDS             complement(<1..577)
                     /locus_tag="VG754_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HWF19660.1"
                     /translation="MPTVASIKSDAPAFAGDPDGPLTSANVRLLDQQFGDSSTEQVLV
                     WSWGRFGKRAAIGTSFQGAGLVMMDLAKRNGIQFPVFTLDTGLLFKETLELKQRLEDF
                     FGFKIESVVPDLSLEQQEDAQGPELWKREPDLCCTIRKVLPLQGKLGELDCWITGLRR
                     QQSDTRADIGIIELYTLDQSSKRDIVKLNPMA"
     gene            complement(582..2273)
                     /locus_tag="VG754_10355"
     CDS             complement(582..2273)
                     /locus_tag="VG754_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HWF19661.1"
                     /translation="MIQSAAPGSPSKLSRNEGLKEACPTLAGHIGPTLANAALERFSD
                     DDYEFLKFHGIYQQDDRDQRKVAKHYIFMVRGRLPGGVVHPEHFLTFDRLATQYGNNT
                     LRITSRQGFQFHGIVKSGLGPMMKAINEAMATTLAACGDVNRNVMAAPTPATNPVVEF
                     IQADAREVSNALLPKTKAYHQIWVEGTELNLEDESAKTFVDPLYGKTYLPRKFKTAFA
                     IPPLNDIDVFTNCLGYIAIVEDGKLAGYNLIAGGGMGMSHGNVQTFPRLADVVGFVTR
                     EQVVPAAKAVVTVHRDFGDRTNRKHARLKYVLEEKGVEWFRSEVEQRMGFKFQPARPY
                     KFTKQGDTFGWQQQLDGNHFLGLYVEAGRIKDTDTTQLKTALRKIVEQFKTEIRLTPS
                     QNLLLVNVKSAERGPINQILTAHGVAVENQATVIRRASMACPALPTCGLALAESERFL
                     PDLLTRIENLLAEVGLPGEEIVIRMTGCPNGCARSSMAELGFIGKAPGKYQIYVGGNE
                     SSTRLNRLYKETIKTEDMVNELRPTFLRFKHERIGSERFGDFAARVLWPEVAVQI"
     gene            2824..3045
                     /locus_tag="VG754_10360"
     CDS             2824..3045
                     /locus_tag="VG754_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19662.1"
                     /translation="MKTVTLRSLRRSTAPLDSAAAGEEIVVTKFGKPYVRILPAQPAK
                     TFLGAARHLAGKKPVSPAPIPKSEWKGLI"
     gene            3068..3457
                     /locus_tag="VG754_10365"
     CDS             3068..3457
                     /locus_tag="VG754_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWF19663.1"
                     /translation="MAQWAFDAGSSARTHSQFLEIGGPNGLCSISLLESAILHRLGRL
                     ELTGSLDEFFAAALSADVQVLELTPAIASKTSALPEQFHGDPFDRTIVATAAVLKLTL
                     ITADPAIRDAKLCPVEYYPFRPSRSGK"
     gene            complement(3468..3893)
                     /locus_tag="VG754_10370"
     CDS             complement(3468..3893)
                     /locus_tag="VG754_10370"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWF19664.1"
                     /translation="MKRTASAVWNGDLKTGKGALSAPGGALKNTPYSFTSRFENGPGT
                     NPEELIAAAHAGCFTMATSAFLGRAGFTPTELSTQATLTLDQVNGNWTITAIHLDLTG
                     RVPGIDAAKFEEIAKDAKANCPVSRVLKADITLSAKLLT"
     gene            4054..4608
                     /locus_tag="VG754_10375"
     CDS             4054..4608
                     /locus_tag="VG754_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWF19665.1"
                     /translation="MLVASEREQLPVAAARAGDAEAWGVLLARFRLPLYAYVFELVHQ
                     EQTSLDIVQETFINAARHIGTLRDDEKFASWLFNIAHQKCIQHWRRVHREEISIDPQD
                     EILEDFEPGPRDLLIRKEQEAEFMRLLDKLAVPHRAVLLLYFVEDFSLEEIAHITGTQ
                     LGTVKSRLHYARKAFKKLFEENQP"
     gene            4605..5039
                     /locus_tag="VG754_10380"
     CDS             4605..5039
                     /locus_tag="VG754_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19666.1"
                     /translation="MKTPREIIMERHRAAETKLDAIRKEELAALACASATTPNTPKNS
                     FWLSSIVERFWRESIWPWRRVWTGIAAVWLGILALNLAAGGTPQPGSSGMTATKNPEV
                     VTALLEQKKLLAQLLEPVVSSTPALPKPAGPRSEQRQELFIA"
     gene            5074..>5256
                     /locus_tag="VG754_10385"
     CDS             5074..>5256
                     /locus_tag="VG754_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19667.1"
                     /translation="MQNPSSDPMTSSTSKHRRGFFSWRTLGLCLIGLAVLVTLIALFY
                     AEEDWRGKHAWEKYKRD"
BASE COUNT         1194 a         1430 c         1425 g         1207 t
ORIGIN      
        1 cggccattgg attcaatttc acgatgtctc gcttgctcga ctggtcgagc gtgtaaagct
       61 caatgatgcc gatgtcggcg cgggtgtcgg attgctggcg gcgcaaaccg gtgatccagc
      121 aatccagttc gccgagcttg ccttgcaatg gcaggacttt gcgaatggtg cagcaaaggt
      181 ccggctcgcg tttccacagt tccggcccct gcgcgtcttc ctgctgttcg aggctcaaat
      241 cgggaacgac ggattctatt ttgaaaccaa aaaaatcttc gaggcgctgc ttgagttcca
      301 aagtttcctt gaacaacaaa ccggtatcga gcgtgaaaac gggaaattgg attccattcc
      361 gtttggccaa atccatcatc accaaacccg cgccttgaaa gctggtgccg atggccgcgc
      421 gtttgccgaa acgtccccac gaccatacca acacctgttc tgtggatgag tcgccgaatt
      481 gctggtccag caagcgaaca tttgcggaag tcaatggacc atcgggatca cccgcaaagg
      541 ctggcgcgtc ggattttatg gaggcaacgg ttggcaagag attagatttg gacggccact
      601 tccggccaca acacgcgcgc cgcgaaatcg ccaaaccgct cgctgccgat gcgttcatgt
      661 ttgaaacgga gaaatgtcgg gcgcagttcg ttgaccatgt cctcggtctt gatggtttct
      721 ttgtaaaggc ggttcaagcg cgtgctcgat tcgttcccgc caacataaat ctggtatttg
      781 ccgggcgctt tgccgatgaa tcccaattcg gccatcgatg aacgcgcgca cccattcggg
      841 caaccggtca tgcggatgac gatttcttcg ccaggcaaac cgacttctgc gagcaagttt
      901 tcgatgcgcg tgagcaaatc aggcaggaac cgttctgatt ccgccaacgc cagaccgcac
      961 gtcggcaatg ccgggcaggc catcgaggcg cggcggatga ccgtcgcctg attttccaca
     1021 gcgactccgt gcgcagtgag aatttgattg atcggcccgc gctcggcgga cttcacattt
     1081 acgagcaaaa gattctgcga aggcgtgaga cggatttctg tcttgaattg ctcgacgatc
     1141 ttgcgcaacg cggtcttgag ctgcgttgtg tcggtgtcct tgatgcgccc cgcttcgacg
     1201 taaaggccga gaaaatgatt gccgtccaac tgctgttgcc agccgaaggt gtcgccttgc
     1261 ttggtgaact tgtacggccg cgcaggctgg aatttgaaac ccatccgttg ctcgacttcg
     1321 ctgcggaacc attccacgcc tttttcttca agcacatatt tcaagcgggc gtgcttgcgg
     1381 ttcgtgcggt cgccgaaatc gcgatgaact gtcacgaccg ccttcgcggc gggaacaact
     1441 tgctcgcgcg tgacaaagcc gacgacatcc gccaggcgcg ggaaggtttg cacgttgccg
     1501 tggctcatgc ccatgccgcc gccagcgatc aaattgtagc ccgcgagttt tccatcctcg
     1561 acaatcgcga tgtatccaag gcagttggtg aagacatcaa tgtcgttcaa cggcggaatg
     1621 gcgaaagcag tcttgaattt gcgcggcaga tacgttttgc cataaagcgg gtccacaaaa
     1681 gtttttgcgg attcatcttc cagattcaat tccgtgcctt cgacccagat ttgatgataa
     1741 gccttggtct tgggcagcaa ggcattcgaa acttcgcgcg cgtccgcttg aatgaactcc
     1801 accacgggat tggtcgcggg cgtgggcgct gccatcacgt tgcggttcac atcgccgcaa
     1861 gcggccaagg tcgtggccat cgcttcgttg atggctttca tcatcgggcc aaggccggac
     1921 ttcacgatgc cgtggaattg aaatccctgg cgcgaggtga tgcgcaacgt gttgttccca
     1981 tattgcgtcg ccaatcggtc gaacgtgagg aaatgctccg gatggaccac cccgccggga
     2041 agccgcccgc gcaccatgaa aatgtaatgc ttggcaacct tgcgctggtc gcggtcatcc
     2101 tgctgataaa tgccgtggaa tttcaaaaat tcgtaatcat catcggaaaa acgctctaga
     2161 gcagcgttcg ccaaggtggg gccaatatgg ccagccagcg ttgggcaagc ttctttaagc
     2221 ccttcattac gagaaagttt tgagggagaa ccgggtgcag cactctgaat cataagttac
     2281 ttacttatct tactaatgtt taacaatcgg ccattttctt tgcttcgtca atgcaatttt
     2341 ttgcattttg ccccgtcccg gcgaatcacc agcctttccg gatcggatag aacgcatcga
     2401 aaataaaagc ctaccgaagc atcattgaat tgcgagcgga aatatatcag gcggaacgcc
     2461 agggccgcat cttgacactg ttcttgagaa tgtacaggta tggtttggat gacgctcgcc
     2521 ctcacccaaa ccctctcccc caagagaggg ctttcaccat ttcccgctgt ttggaatatt
     2581 cccgcgatgg aatgatgcaa accattgcgg ccaagataaa gcgggcaggt gtgccggttc
     2641 tccttctcct agggggagaa ggccgggatg agggcgagct ttctctcgct tctcacaatg
     2701 cattcaagaa cagtcaggat gcgcccgaat gccagcgcaa acgaatcatg aaatgaccgc
     2761 cctgcacgag ccgggacaaa gtttttcttg atgaagggag gattgtctga caaactgtct
     2821 gacatgaaga ctgtgacgct ccgctcgttg cgcagaagca ccgctcctct ggattcggct
     2881 gccgcgggag aagaaattgt ggtcaccaaa ttcggcaagc catacgtgcg gatccttccc
     2941 gcacagccgg ctaagacttt cctgggcgcg gcgcggcatt tagcgggcaa gaaaccagta
     3001 tcgcccgctc ccattccaaa atcggaatgg aaaggtttga tctgaagtac ttgttggata
     3061 cagcgccatg gctcaatggg cttttgatgc cggaagttct gcccgaacgc attcgcaatt
     3121 tttggagatc ggcgggccga acggtctatg cagcatcagt ctgcttgaat cagcaatatt
     3181 gcaccggctg ggccgattgg aattgacggg ttcattggat gaattttttg cagcagctct
     3241 ctccgctgac gtgcaggtgc tggaattgac tcctgccatt gcgtcgaaaa ccagcgcttt
     3301 gccggaacaa tttcatggcg acccgtttga tcgaaccatc gtcgcgactg ccgctgtgct
     3361 gaagttgaca ctcatcaccg ccgatcccgc cattcgcgac gccaaactgt gcccggtaga
     3421 atattatccg ttcaggccat cgcgctccgg gaagtaaaca ggctttttta cgtcagcagc
     3481 ttcgcggaaa gtgtgatgtc cgctttcaac acccgcgaca ccgggcagtt cgccttggca
     3541 tctttggcga tttcctcaaa ttttgcggca tcaattcccg gcacgcgacc cgtcaaatca
     3601 agatggatgg ccgtgatggt ccagttgccg ttcacctggt cgagagtaag tgtggcttgc
     3661 gtggacaatt ccgtcggcgt gaaaccggcg cgtcccaaga acgccgaagt tgccatggta
     3721 aagcagccgg catgcgcagc ggcgatgagt tcctcgggat ttgtgcccgg cccattttca
     3781 aagcgcgatg tgaaagaata aggcgtgttc ttcaatgcgc cgccgggcgc tgaaagagca
     3841 ccctttccgg ttttgagatc gccgttccag acggcggaag cagttcgttt cataatttta
     3901 cagtccaggt gaatccgatg cggcagttag ttaaacaaac atcagcacaa gaacgctctt
     3961 ttgtgaagca ctaaaagcaa ggggcttcac acgaaaacat tcattcccca acattgaacc
     4021 ttttcaccga ttgcgcattc tatcttggtg aaaatgctcg tggcgtctga acgcgaacaa
     4081 ctgccggtgg ctgctgcccg cgccggagat gcggaagctt ggggcgtgtt gctcgcgcgc
     4141 tttcgattac ccctttatgc gtatgttttc gaactcgtcc atcaggaaca aaccagcctg
     4201 gacatcgtcc aggaaacgtt catcaacgcc gcgcgccaca tcggcacatt gcgcgatgac
     4261 gaaaaatttg cctcgtggct cttcaacatc gcgcatcaaa aatgcattca acattggcgt
     4321 cgggtgcacc gggaggaaat ttcgattgac ccgcaggacg aaattttgga ggacttcgaa
     4381 cccggcccgc gcgacctgct catccgcaag gaacaggaag ctgaattcat gcggctgctg
     4441 gacaaacttg ccgtgccgca tcgcgccgtc ctgttgctct atttcgtcga ggatttttca
     4501 cttgaagaaa tcgcccacat cactgggacg caacttggga ctgtcaaatc ccgcctgcat
     4561 tacgcccgca aagcttttaa aaaattattc gaggagaacc aaccatgaaa accccgcgtg
     4621 aaatcatcat ggaacggcat cgcgccgctg aaacgaaact cgacgccatc cgaaaggaag
     4681 aactcgcagc attggcctgc gcatccgcga caacccccaa cacgccaaaa aattcattct
     4741 ggctgagttc aatagtcgaa agattttggc gcgagtccat ttggccatgg cgtcgcgtgt
     4801 ggaccggaat tgccgcggtg tggctcggca ttctggcgtt gaatctggcg gctggtggaa
     4861 cacctcagcc gggcagcagc ggcatgactg caacaaagaa ccctgaagtt gtaaccgcat
     4921 tgctcgaaca gaaaaaattg ctggcgcaat tattggagcc agtcgtgtcg tccactccag
     4981 ctttaccaaa accggccggt ccgcgcagcg agcagcgcca ggaattgttc attgcctgag
     5041 tcatcggcat ttgtttaggg agcaaaaaat attatgcaaa atccatcatc ggacccgatg
     5101 acatcatcaa cttccaagca tcgtcgtgga tttttcagtt ggcggacttt gggattgtgt
     5161 ctgataggtc ttgccgtgct ggtgactttg attgcgctct tctacgccga ggaggattgg
     5221 agggggaaac atgcgtggga aaaatacaag cgggac
//
LOCUS       DASZMM010000432         6129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5405204, whole genome shotgun sequence.
ACCESSION   DASZMM010000432 DASZMM010000000
VERSION     DASZMM010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5405204"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VG754_10390"
     CDS             <1..230
                     /locus_tag="VG754_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWF19668.1"
                     /translation="NLTPLEVKTIITRVGHGTKIVFTGDPYQIDNPYVDSSSNGFNYI
                     ISKFRAESVAAHIELQKGERSELAELAANLL"
     gene            243..1883
                     /locus_tag="VG754_10395"
     CDS             243..1883
                     /locus_tag="VG754_10395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWF19669.1"
                     /translation="MDLPATTSERKERRPLETGKWSFRNPGAWLSCIGVIVLFVALRW
                     NNYDAPLIRDEGEYFYSAALLKQGIAPYVNSFVQKPPMIIYTYAFATTVLPNSYWSAR
                     FLAYLFVALTTVLLGIIARFEFGPGVALTAMWLFTPMILQPAVEQFCANTEMFLLLPL
                     TAMLALLAWKRRSGGGGAMVWFVAGFLAAITILYKYTAFPLLLVIFGSWSTCKLRTRD
                     ISGLFSNWLFAALGALTATVAVLGFFAAKGGLTEFLNCTILFNRFYAASGSFSSAEGF
                     YSRLGLYATDWGILLLGPLYFFSQPGCKEPRIWFWMGLFVTAWITTSISGYGQYYILV
                     MPFWAVLCAVAANRFSAWLAAKKKWNANMVRYPIIVTVVLLACAPDLSWILRTKEQFA
                     SNKRVEYSNPFRESETVAKHVAEMTSPDDTVYVAGSEPQILFYAHRRSATRFIIAYPL
                     MIPTPLASLYQKEAMADLEKRRPKVMVFATMSQSWLQQDGTPPEFLAYLKNLMRDHYD
                     LAGGSVRDGNEWRWKEPIGPQDVPHATLLLLRRKPGEQ"
     gene            1969..2502
                     /locus_tag="VG754_10400"
     CDS             1969..2502
                     /locus_tag="VG754_10400"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HWF19670.1"
                     /translation="MSGFLRFVGIVNAAIWFGAAIFFAVGVLPGVFSEEMHLLFHESA
                     TDPFYSGAVAQALFQRYFIFQCICGAVALIHLIAEKLYLGRALPKWGMGLVIALFCFG
                     LMGSLWLQPHMKELRQTRYFGRTQDEKEHARHSFALWHGFSEVANFLIIAGLLVHLVR
                     VTRSAVPGRYGTFYQIP"
     gene            2580..2885
                     /locus_tag="VG754_10405"
     CDS             2580..2885
                     /locus_tag="VG754_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PUR family DNA/RNA-binding protein"
                     /protein_id="HWF19671.1"
                     /translation="MISNDRPSPYGNRAYGSPYPPKPPVNEDTLKTDKVQIERKTFVF
                     TLKENPRGRFLRITEDVGGRRDTIIIPAPGLEEFKKLLDQMVKASTEAPPSKEEKGG"
     gene            2962..3432
                     /locus_tag="VG754_10410"
     CDS             2962..3432
                     /locus_tag="VG754_10410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWF19672.1"
                     /translation="MRELPHTASCFVCGESNKSGLNLRFETDGQIVRAKFLPRPEHIG
                     FKQTIHGGIIATILDEIMVWACAVQTRRFAYCAELNVRFLNPLRPGEETVASAELVAN
                     RKNKLFEAKGELRDHSGQLLATATGKYLPIKESDAAAMFDDFIGDAKEWLGQAR"
     gene            complement(3447..5033)
                     /locus_tag="VG754_10415"
     CDS             complement(3447..5033)
                     /locus_tag="VG754_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF19673.1"
                     /translation="MQLKRIFCVVLSSGVTLLANAEPAQTAQTNQPPSTNQASQALEP
                     AHTRKTRLLTLDECIKIALEHNLDIQIQEFNPIIDQYALNVNFGTYEPLFTFSGTKSY
                     NDTTGGLNPQTQTPFGGNVSEADTYVPGIKGVLPTGLQYDISGNLQRISELGAINSNT
                     PLHYLQPFFPATAGISLTQPLLKNFWIDNTRLQIQLSRATLKISEAALRLQIMTTVTS
                     VKSAYYNLLYGRDNVRANQTAYQLAEQLVNENLKRVEVGALAPLDEKQSESQAAASLA
                     AVHAAEFQLGGYENTLKNLLTDNYSEWADVTIIPAEELVAVPQDVNLQESWRRAVAER
                     PDLIEAKLRVEAQNVTLKYDFNQLFPELDVTGSYGRNATDATFDDTLADLRRGSHSYY
                     SYGAFVSIPLGGNYTARNQYKSGKATLKQLLLTLKQTEQTILIAVDNDVGNIQSTLQQ
                     AYATRDARIYAQEALDAERKKLENGKSTSFIVLQLISNLTTARVAEIQALANYNIAVS
                     QLALDEGSTLEANHIQLNVK"
     gene            complement(5148..>6129)
                     /locus_tag="VG754_10420"
     CDS             complement(5148..>6129)
                     /locus_tag="VG754_10420"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWF19674.1"
                     /translation="KTAVKRAAELLRAGEVVALPTETVYGLAANALNPEAVARIYEIK
                     GRPAYNPIIVHVSDIEMARRCVAQWPMAAESLARAFWPGPLTLVLRCSKEIPEIVTAG
                     GSTVGVRWPSHPFIQAVIRECDFPLAAPSANPSNQVSPTNAAHVSKGLGNKIPLIIDG
                     GQSQVGIESTVLDLSTELARVLRPGIIHGESLRAAIADCGMRIADCEEAKREHSPLRS
                     PGRLPRHYSPKARLEVLSWRDTAELHSQIKNQKLKLAAIHVIAHTVIPSGEGLGRVSV
                     IPHDAEAFARAIYAELHQCDELGAERIIVEALPETNEWLAIADRLKRAAG"
BASE COUNT         1308 a         1596 c         1690 g         1535 t
ORIGIN      
        1 aaaacctgac tccgctggaa gtcaagacca tcatcacccg cgtcggtcat ggcacgaaga
       61 ttgtgttcac tggcgatccg taccagattg acaatcctta cgtggactct tcctcgaacg
      121 gtttcaatta tatcatcagc aagttccgcg cggaatcggt ggccgcgcac atcgagctgc
      181 aaaaaggcga gcgttctgaa ctggcagaac tggcggccaa tttgttataa gacagtccag
      241 taatggacct gcctgccacg acgagcgaac gcaaagaaag aagacccctc gaaaccggga
      301 agtggtcctt tcgcaatccc ggcgcctggc tcagttgcat cggcgtaatt gttttgttcg
      361 tcgctcttcg ttggaataac tacgacgcgc cgttaatccg ggacgagggg gaatatttct
      421 attcggctgc gttgctgaag caggggatcg cgccttacgt gaattccttt gtgcaaaagc
      481 ccccgatgat catttacaca tacgcgttcg ccacgacagt attgcctaat tcatattggt
      541 ctgcgcgttt tctcgcatat ctgtttgtgg ctctgacgac ggtgctgctg ggaatcatcg
      601 ctcggtttga attcggcccg ggcgttgcgc tcactgccat gtggctcttc acgcccatga
      661 tattgcaacc ggctgtggaa cagttttgtg cgaacacgga gatgttcctg cttttgccgc
      721 tgacggcgat gctcgcattg cttgcatgga agcgaagaag tggcggcggc ggagccatgg
      781 tttggtttgt cgcaggtttt ttggcggcga taaccatctt gtataaatat accgcattcc
      841 cgctgctgct cgtgattttt ggttcctggt caacctgcaa attacggacg cgcgacataa
      901 gcggcttatt ttcaaattgg ttatttgccg cgctgggcgc actcactgcc acagtggccg
      961 tgctgggctt tttcgcggca aaaggtggac tgacggaatt cttgaattgc accatcctct
     1021 tcaaccgttt ttatgctgca tctggcagtt tcagttcggc ggaaggcttt tattccaggc
     1081 tgggcttgta tgcgacggat tggggcattc tgttgcttgg gccgttatat tttttcagcc
     1141 agcccggttg caaagaaccg aggatttggt tttggatggg actttttgta acggcatgga
     1201 tcacgaccag cataagcggg tatggacaat actacattct ggtgatgcct ttttgggctg
     1261 tgttgtgcgc ggtcgcagcg aatcgctttt cagcgtggct tgccgcgaaa aaaaaatgga
     1321 acgcgaacat ggtgcgttat ccgatcatcg tcacagtggt cctgttggcg tgcgctcctg
     1381 atttgagttg gatcctgcgg acgaaggaac agttcgcttc gaacaaacgg gtcgagtaca
     1441 gcaatccatt ccgcgaatcg gaaactgttg caaaacacgt tgctgaaatg acctcgccgg
     1501 atgataccgt ctatgtcgcc ggttctgaac cgcagatttt gttctacgcc caccggcgaa
     1561 gcgcgacgcg tttcatcatc gcttatccgt tgatgatccc gacgcctctg gcaagtttgt
     1621 atcaaaagga agccatggcg gacctggaaa aaaggcgtcc caaggtgatg gtttttgcaa
     1681 caatgagcca aagctggctt cagcaggacg gcacgccccc agaattcctt gcctacctca
     1741 aaaatttgat gcgtgaccat tacgatctgg ccggagggag cgtgcgggac gggaacgaat
     1801 ggcgctggaa agaaccgatt ggaccccaag acgtgccgca cgcgactttg ttgttgctcc
     1861 gtcgcaagcc gggtgagcag taattgccgg agtaattcat tcccatgctg cgcagtggcg
     1921 tgtgaaaact tttcttggaa ctctctttta cgaccctata gtaagccggt gagcggcttt
     1981 ttgagatttg tgggaatcgt caacgcggcc atctggtttg gcgcggccat tttttttgcg
     2041 gtgggagttc tccccggggt tttttccgag gagatgcacc ttttgtttca tgaaagcgca
     2101 acggacccgt tttactccgg ggcggtcgcg caagcgcttt tccaacggta tttcatattt
     2161 caatgcattt gcggcgcggt cgcattgatt catcttattg cggaaaaact ttacttgggc
     2221 cgcgccctgc cgaagtgggg catgggattg gtcattgcgt tgttctgctt tggattgatg
     2281 ggcagcttgt ggctgcagcc gcacatgaag gagttgcggc aaaccagata cttcggacga
     2341 acgcaggacg aaaaggaaca cgcccggcat tccttcgcac tctggcatgg cttttccgag
     2401 gtcgccaact tcttaataat cgcaggtttg ctcgtccatc tcgtccgcgt cactcggtct
     2461 gcagtcccag gcagatacgg gaccttctac caaattccgt agttgacaaa ttccatttct
     2521 ggcgaagttt gatatatggc ttatcttgtt gtttaggtaa accattttaa aaaattgata
     2581 tgatttcgaa tgatcgtccg tcaccctacg ggaaccgtgc ttacggttcg ccgtatccgc
     2641 caaagccgcc tgtaaacgag gacacgttga agacggacaa agtccaaatt gagcgcaaaa
     2701 cgttcgtgtt cacgctcaag gaaaacccac gcggccgttt tttgcgcatc accgaggatg
     2761 tcggcgggcg cagggacacc atcatcattc ccgcgccggg attggaagaa ttcaaaaagc
     2821 ttttggatca aatggtgaaa gcctcgaccg aagctccccc ctccaaggaa gaaaaaggcg
     2881 gataacagtt tatccgatat gccgggcgcg attctccgga attgaattgc gtcatcgttt
     2941 cgagtttgtc tccaatcagt catgcgcgaa ctgccccata ccgcgtcttg ttttgtttgt
     3001 ggtgaatcca ataaaagcgg actaaacctc cggttcgaaa ctgacggtca aatcgttcgc
     3061 gcaaaattcc tgccgcgacc cgaacacatc ggcttcaagc agaccatcca cggtggaatc
     3121 attgccacaa tccttgatga aatcatggta tgggcttgcg cggtgcaaac gcggcggttc
     3181 gcctattgcg cggagttgaa tgtccgtttt ctaaatcctt tgcgtccggg cgaggaaacc
     3241 gtggcaagcg ccgaattggt cgcaaaccgc aaaaataaat tgttcgaagc caagggcgaa
     3301 ctgcgcgacc attccggtca gttgctcgcc acagccaccg gaaaatatct tcccatcaag
     3361 gagagcgatg ccgcggcgat gttcgatgat tttattggcg atgccaaaga atggttgggc
     3421 caagctcgat gaggcgggag gcggacttat ttcacgttca actgaatatg gttcgcttca
     3481 agcgtgctgc cttcatcgag cgcgagctgt gagacggcga tgttgtagtt ggccagcgcc
     3541 tggatttcag cgacccgcgc ggtggtcagg tttgaaatca gttgcagcac gatgaagctg
     3601 gtgctcttcc cattttccag tttcttgcgt tcggcatcga gcgcttcctg ggcgtaaatc
     3661 cgtgcatcac gcgttgcata agcctgctgc aacgtggact ggatgttgcc gacgtcgttg
     3721 tccaccgcaa tgaggatggt ctgttcggtt tgtttcaggg tgagcaataa ttgcttcaaa
     3781 gtcgccttgc ctgatttgta ctggttgcgc gcggtgtagt tgccgccgag tggaatgctc
     3841 acaaacgcgc catacgaata ataagaatga ctgccccggc gcaaatcggc gagggtgtcg
     3901 tcgaatgtcg cgtcggtggc attgcgccca tagctgccgg tcacgtccaa ttccgggaaa
     3961 agctggttga aatcatattt caacgtgaca ttctgcgctt ccacgcgaag cttggcctcg
     4021 atcaaatcag ggcgttcagc aaccgcacgc cgccagcttt cttgcaaatt tacgtcttgc
     4081 ggcacggcaa ccaattcctc agccgggatg atggtgacat ccgcccattc tgaataattg
     4141 tcagtgagca aattcttcaa tgtgttttcg tacccgccaa gctggaactc cgcggcatgc
     4201 acggcggcaa ggctggcggc cgcctgggat tcggattgtt tttcgtccag cggcgccagc
     4261 gcgcccactt ccacgcgctt aagattttcg ttcaccaact gctccgccaa ttggtaagcg
     4321 gtctggttgg ccctcacgtt gtcgcggcca taaagaagat tgtaataggc ggatttgacc
     4381 gacgtgaccg tggtcatgat ttgcagacgc aatgccgctt ccgaaatttt caaagtggcc
     4441 ctgctgagtt ggatttgcag gcgcgtgttg tctatccaga aatttttcaa caaaggttgc
     4501 gtgagtgaaa tacccgccgt cgcggggaag aacggctgca ggtaatgcaa cggtgtattg
     4561 gagttgatgg cgccaagctc gctgatgcgc tgaaggtttc cactgatgtc gtattgcagg
     4621 ccggtcggca aaacgccctt gatgccggga acgtaggtgt ccgcctcact gacattgccg
     4681 ccgaatgggg tttgggtttg aggattcagg ccccctgtcg tatcgttgta gctcttcgtt
     4741 ccgctaaagg tgaacaacgg ctcgtaagtt ccaaaattca cattgagcgc atattgatcg
     4801 atgatcggat taaattcctg gatctggatg tccaaattgt gttccaaggc aatcttgatg
     4861 cattcatcca gcgtcaataa gcgtgtcttg cgggtgtgcg ccggttccaa cgcctggctg
     4921 gcctggttgg tcgatggcgg ttggttcgtt tgcgcggtct gcgccggctc cgcgttggca
     4981 aggagcgtca cgccggaaga caacacgaca cagaaaattc gcttcagttg catcaagtcc
     5041 gaagagtgct tgattcgttt tgattcgtca aacaatcgca tgggtcagtt cttttggttt
     5101 ttcgttctga aaacgcatgt ttttattaga aattttttct catcaaatca gccggctgct
     5161 cgtttcagac ggtcagcgat ggcaagccat tcattcgttt caggcaatgc ttccacgatg
     5221 atccgctccg cgcccagttc atcgcattga tgcaactcgg cgtaaatggc ccgcgcgaac
     5281 gcctcggcat cgtgcggaat cacgctcacg cgcccgagtc cttcgccgga aggaatcacc
     5341 gtgtgcgcga tgacgtggat ggcagccagt ttcaattttt gatttttgat ttgtgaatgc
     5401 aactctgccg tgtcgcgcca ggaaaggacc tccaacctgg ccttcggcga ataatgacgc
     5461 ggcaatcgcc ccggactgcg caaagggctg tgttcgcgct ttgcctcttc acaatccgca
     5521 atcctcattc cacaatccgc aatcgcggcg cgcagcgatt ccccgtgaat gattcccggc
     5581 cgaagcacac gcgcgagttc cgtgctcaaa tcaaggactg tggattcaat tcccacctgc
     5641 gactgcccgc cgtcaatgat gagcgggatt ttatttccca agcctttgct gacgtgcgcg
     5701 gcgttggtcg gggaaacctg gttggacgga tttgcactgg gcgcagccag cggaaaatca
     5761 cattcacgga tcaccgcctg tatgaacgga tggctcggcc agcgtactcc aaccgtcgaa
     5821 ccgccggcgg tgacgatctc cggaatttcc ttggaacatc gcaaaaccag cgtgagcggg
     5881 ccgggccaga aagcgcgcgc aagcgattca gcggccatcg gccattgagc gacgcaacgt
     5941 ctggccatct caatgtccga cacatgaacg atgatgggat tatatgcagg gcgtcctttg
     6001 atttcgtaaa tccgcgcgac cgcctccgga ttgagcgcgt tggccgcaag gccataaacg
     6061 gtttccgttg gcaatgccac gacttcgccg gcgcgcaaca attcggcggc gcgtttcacc
     6121 gcggtttta
//
LOCUS       DASZMM010000433         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1313474, whole genome shotgun sequence.
ACCESSION   DASZMM010000433 DASZMM010000000
VERSION     DASZMM010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313474"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1200)
                     /locus_tag="VG754_10425"
     CDS             complement(262..1200)
                     /locus_tag="VG754_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWF19675.1"
                     /translation="MNRREMILRTGAAALGLGLTGCSSLNQTTSSKTRKVLFFSKSSG
                     FEHSVIKSVNGKPSFAQQVLTELAPKHNIEFTFSKDGSLFTPPYLAQFDAYFFYTTGN
                     LTEPGTDKNPPMTPEGKTAFLDAIKHGKGFIGTHAATDTFHTGESMDVPKGEVPPRDK
                     NYGEKADPYVRMIGAEFIRHDSQQMAKLKVIDHKFPGFKALGEELEIKDEWYALKDFS
                     DNLHVLLVQETEGMTGPHYQRAPYPETWARMHGKGRVFYTSMGHREDVWTNPVFQEIL
                     FGGITWAVRNANADVTPNIAQVTPHCMEIPLETPKK"
     gene            1660..>3000
                     /locus_tag="VG754_10430"
     CDS             1660..>3000
                     /locus_tag="VG754_10430"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HWF19676.1"
                     /translation="MASVKIFRSLPGLVLGCALALFSIATSAIAAPQLTLSPHHVPAA
                     VANLQPIGRLPASEQLTIAIGLPLRNQDTLSNLLEQLYDPASTNYHHFLEPQQFAQMF
                     GPTPQDYQALIDFAQTNGLNVKKTHSNRALLTVNGAVSNIEKAFHIALHVYRHPTEDR
                     TFYAPATEPTLDFNVPVLNISGLDNWIVPRPMSLHPMPVDAAKPSATTGSGPSGNFIG
                     KDFGAAYIPGVALTGTGQSVALVEFDGYYAVDINNYEALAGLPNVTLTNITPDGPITI
                     GSGNIEVALDIEMAISLAPGLTKVAVYEGNVPDDVLNQIATDKLANQISASWTYGIDS
                     TTVSIFQQYAAQGQSFFNASGDSGAYTDTHSGRNPAPTPTDDPYITIVGGTSLTTTGP
                     GGSYVSENTWSWFNTGQGTSASSGGRSTRYSIPAWQSGISMTANHGSTTARNLPD"
BASE COUNT          666 a          829 c          798 g          707 t
ORIGIN      
        1 cgcgctttcg tgggaagaat gcctgtttct cccatgcgtt gatattctgc cacagtaaaa
       61 cgatgactct tctctacaag cataaccaac ggcaatttac ctaatcacta ccgcatcttc
      121 aatccaatct tgcagcctgg caacggtgcg cattcgaaga cttttctgga gcggggactg
      181 gaaagtcccc gcaacccgca ggctggaaag cctgcgccac gtagcgcaga catggtttcg
      241 acgttgcgcc caattttccg gttatttttt cggggtctcc agcgggattt ccatgcaatg
      301 gggcgtgact tgggcgatgt tgggcgtcac gtctgcattc gcgttgcgca cggcccaggt
      361 gatgccgcca aaaaggattt cctggaacac cgggttggtc cacacatcct cgcgatggcc
      421 catcgaggtg taaaacaccc ggcctttgcc gtgcatccgc gcccaagtct cgggataagg
      481 cgcgcgttgg taatgcgggc cggtcatgcc ttcggtttcc tgcacgagga ggacatggag
      541 gttgtccgag aaatctttca aagcatacca ctcgtccttg atctcaagct cttcgccaag
      601 ggctttgaaa cctggaaatt tgtggtcaat tacttttaat tttgccatct gctgcgagtc
      661 gtggcggatg aattccgcgc cgatcattcg gacatacgga tcggccttct cgccgtaatt
      721 cttgtcgcgc ggcgggacct cgcctttcgg cacatccatg ctttcgcctg tgtggaaggt
      781 gtcggtggcg gcgtgcgtgc cgatgaaacc tttgccatgc ttgatggcat cgaggaacgc
      841 ggtcttgcct tcgggcgtca tgggcggatt tttgtccgtg cccggctcgg tcaaattgcc
      901 ggtggtgtaa aaaaagtacg cgtcgaattg cgcgagatag ggcggcgtga aaagactgcc
      961 gtccttcgag aaggtgaatt cgatgttgtg cttgggcgcg agttcagtga ggacttgctg
     1021 ggcgaagctg ggcttgccgt tcacggactt gatgaccgaa tgctcgaagc ctgatgactt
     1081 gctgaagaaa aggactttcc tggttttcga tgaagtggtt tggttcaaag aggagcaacc
     1141 cgtcaagccg aggccaagcg cggccgctcc tgtgcggagg atcatttctc gtcggttcat
     1201 gggcatatca ttcaaaggcg cggagcggga gtcaattgct aagacgttaa atcgttaaat
     1261 ggttaaatcg ttacagtagc atcccttagc ggcgtcaagc cagtcggaac ctgcgatttg
     1321 attggcaaaa ccaggtgaac ggcagaaaat gagattaaga gttttttatt aggaaattat
     1381 gaaatcagga acaaaattct tgaggaacat cgtcttttcc tgctttccta attcaatttt
     1441 cccaccggat ggctctgtaa ctttaacctt tttaccattt acatttaaca accaccaaat
     1501 ccggcttgta attttctctt aaggcctata taaagatggc tgttctttgc ccccggtgga
     1561 aatccctttt ggcataataa ttgcgcccaa atgtgatttc tagtttttga gacgcggaaa
     1621 agccggctgc cacggcaaat cccggaaatc ctaatttgca tggcatcagt caaaatattc
     1681 agaagtttgc ccggccttgt ccttggctgc gctttggcgc ttttttcaat cgcaacctcc
     1741 gccattgccg cgccgcaact caccctttcg ccgcatcatg tgccggcagc ggtcgcaaat
     1801 ctgcaaccga ttggccggtt gcctgcgtcc gagcagttga ccatcgcgat tggtttgccg
     1861 ctgcgaaacc aggacacgct ctccaatctc cttgagcaac tctacgaccc ggcgagcacg
     1921 aattatcatc acttcctcga accgcaacag ttcgcccaga tgttcggccc gacgccgcag
     1981 gattaccagg cactgattga tttcgcgcag accaacggtt tgaacgtcaa gaaaacccat
     2041 tccaaccggg ctctgctcac cgtcaacggc gctgtgagca acatcgagaa ggcgtttcac
     2101 atcgctttgc atgtttatcg gcatccgaca gaggaccgca cgttttatgc gccagccacg
     2161 gagccgacgc tcgatttcaa cgtgccggtg ttgaacatca gcggtcttga taattggatt
     2221 gtcccgcgcc cgatgagcct tcatccgatg cctgtggatg ccgccaagcc gagcgccacg
     2281 actggttccg ggccatccgg caatttcatc ggaaaagatt ttggcgcggc atacatcccg
     2341 ggcgttgcgc tgacgggcac gggacaatca gtcgcgctgg tcgaatttga cggctattac
     2401 gcggtcgata tcaacaatta cgaagccttg gccggattgc cgaatgtcac tttgaccaac
     2461 atcaccccgg acggcccgat aacgatcggc agcgggaaca tcgaagtcgc gctcgacatt
     2521 gagatggcca tttcgcttgc gccgggattg acgaaggttg ccgtctatga agggaatgtt
     2581 cctgacgatg tgctcaatca aatcgccaca gacaaattgg caaaccaaat cagcgcgtcg
     2641 tggacttatg gcatcgattc aaccaccgtc tcgatcttcc agcaatatgc cgcgcaaggc
     2701 caaagtttct tcaatgcctc cggcgacagc ggagcttaca cagacactca ctcgggccgc
     2761 aatccggctc ccacgccgac cgatgatccg tacatcacga ttgtcggcgg aacttcgctg
     2821 accacgaccg ggccgggcgg ttcgtatgtt tcggaaaaca cgtggagctg gttcaacacc
     2881 ggccagggaa ccagtgcgtc cagcggcggg agaagcaccc gatatagcat cccagcatgg
     2941 caatcaggca tcagcatgac agccaaccac ggctcaacta cggcccgcaa cctgccggac
//
LOCUS       DASZMM010000434         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4318372, whole genome shotgun sequence.
ACCESSION   DASZMM010000434 DASZMM010000000
VERSION     DASZMM010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4318372"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VG754_10435"
     CDS             <1..210
                     /locus_tag="VG754_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWF19677.1"
                     /translation="NVGKRSRSLAQQLLDDANLCKVLSAQGLFVLGHTKRDTLEIPPA
                     LQEVKVLKHGDSVMRLLKLAGKASA"
     gene            361..966
                     /locus_tag="VG754_10440"
     CDS             361..966
                     /locus_tag="VG754_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19678.1"
                     /translation="MASKKYGYGSPPFLLLLGISALLLSSCAAPQGGVGSGATSVAVY
                     RINVMRFTSPVEVVYWPFDMSNSAHSEGAALNIVTAMQRGDVDGWLNCWAPDERPHPD
                     AAERQKLLEKWAALKDGHINVLGRVVAGVDLIEEISVQEPSQRELLQFPLKNEGGKWW
                     LINMDPDSEYMRWESSPSKLVERVDSMAFRHYLKDVNAKVN"
     gene            complement(1137..2639)
                     /locus_tag="VG754_10445"
     CDS             complement(1137..2639)
                     /locus_tag="VG754_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF19679.1"
                     /translation="MAKVLLVDDELTMVQMVTEMLRQEGHEVFPFTNSKDAMAALEAN
                     APEIVITDLYLDKTRAHGLEILQRARSLPSPASVIVITAFGSIETAVEAMKSGAYDYL
                     EKPFKLDELNLCIQRALSYNVAISENLYLRKQLKKKYQFSQLIGTSTPMQGVFKMIER
                     VADTDSTILILGESGTGKELVARALHFNSRRQFAPFIPVNCSALPENLLESELFGHRR
                     GSFTGAVADKKGLFQEADGGTIFLDEVGSMSQMLQSRLLRVLQEREVRRVGDNIPTYI
                     NVRVLAATNEPLEKKIKDGSFREDLYYRLNVISINLPSIRERREDIPLLVAHFLRNKI
                     NPRSGQPIQITRQAMNLLSAHDWPGNVRELENAIERASTLCENNLIQVSDLPPSLTRH
                     LAQQAAEPAASTEIAQLPVPESAGAKSLYPQTANAAPVGTAPSSTGTPAAPTDINTDL
                     PLKDFLREQELNYLNRILAQLGGDKEKTALHLGISLATLYRKLSEDDRVN"
     gene            complement(2693..3229)
                     /locus_tag="VG754_10450"
     CDS             complement(2693..3229)
                     /locus_tag="VG754_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19680.1"
                     /translation="MQEPTHILAGVIFQRALLRKWSRKIMFVPMAILAFLSHGILDKL
                     ANVTYHPRRADFHSVVWDSYHLTVLVFTILFLCFWWRSFKWGILFAMLPDLDWVFIHG
                     QEIFGLRLSWYQQPHLHHFLGWILANIPGLAWLDHLPNNREHWWGALFELLLVIGMLA
                     LIRFMTSKHESEPTVKPV"
     gene            complement(3234..>3707)
                     /locus_tag="VG754_10455"
     CDS             complement(3234..>3707)
                     /locus_tag="VG754_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HWF19681.1"
                     /translation="EDFYIGSASVKLFLPIFKMNFPEIVDIALPAEGVFHNLVFVSIK
                     KTYPMQAYKIMHGLWGMGQMMFSKYIVVVDADVNVHNTSEVLFRLCANTDPQRDSIFT
                     KGPSDVLDHATSQLAAGSKIGIDATHKLPGEGFSRDWPPLIRMDEATRKKIDGMK"
BASE COUNT          848 a          931 c         1068 g          860 t
ORIGIN      
        1 aatgttggca aacgctcgcg ttcgcttgcg caacagcttt tggatgacgc gaacctttgc
       61 aaagtgctta gcgcgcaggg attgtttgtg ctgggtcata ccaaacgcga cacgctggaa
      121 attccgcccg cattgcaaga ggtcaaagtg ctgaagcacg gcgacagcgt gatgcggtta
      181 ttgaaattgg ccgggaaggc gtccgcttga tgcaatccat ccttgcaaaa aacatccaac
      241 gatgaacact gaacgccgaa catgacaaac tggcgcaatt tcgttgggag ttcgttgttc
      301 gatgttcagc gttcgatgtt tcccattagt tcaattgcga taagccaaaa gaaaatgatt
      361 atggcctcaa aaaaatacgg ttacggttcc ccgccgttcc tgctgctgct tggaatttcc
      421 gcgctgttgc tttcatcctg cgcggcgccg cagggaggag tcggttcggg agccacctcg
      481 gtggcggtct atcgcatcaa tgtgatgcgc ttcacgtcac cggttgaagt ggtgtattgg
      541 ccgttcgaca tgtcaaactc ggcccattct gaaggggccg cgttgaacat cgtgaccgcc
      601 atgcaacgcg gtgatgtgga tggctggctg aattgctggg cacccgacga acgaccgcac
      661 ccggacgccg ccgaacggca gaagcttctg gaaaaatggg cggcgctcaa ggacggtcac
      721 atcaatgtcc ttggcagagt cgtggcgggg gtggatttga tcgaggaaat ttctgtccag
      781 gaaccttcgc aacgggaact gcttcaattc ccgctcaaga atgaaggtgg caaatggtgg
      841 ctgataaaca tggatcccga tagcgaatat atgcgctggg aatcttctcc ctcgaaactt
      901 gtcgagcggg tggattcgat ggcgttccgt cattacctga aagatgtgaa cgcgaaggtg
      961 aattaaaaag gccagcccca cccattggga ctggccttca cctatgatta actatcctgc
     1021 atagtgtctg actatccggc aggagttttt atttaaaaat ttttcaaaaa attttgttcg
     1081 gattaaagag acattgggga cgaattgctt gcaaattgca acaccttacc ccgaaattaa
     1141 ttcacacgat catcttcgga aagtttgcga taaagcgtgg cgaggctgat gccaagatgc
     1201 agcgcggtct tttccttgtc gccgcccaat tgcgccaaaa tgcggttcaa ataattcagt
     1261 tcctgctcgc gcaagaaatc cttgagcggc aaatcagtat tgatgtctgt tggcgcggcg
     1321 ggagttccgg ttgaactggg ggccgttcca acgggcgcgg cgtttgcagt ttgcggatac
     1381 aaactttttg cgcctgcgga ctccggcacg ggaagctggg caatctcggt cgaagcggcg
     1441 ggttcagcgg cttgctgcgc aagatggcgc gtcaggctgg gcggaagatc gctgacctgg
     1501 atgagattgt tctcgcacaa cgtgctggcg cgttcgatgg cgttctccaa ttcacgcacg
     1561 ttgcccggcc aatcgtgcgc cgaaagcagg ttcatggcct ggcgggtgat ttggatgggc
     1621 tggccggaac gcgggttgat cttgttgcgc agaaaatggg cgaccagcaa tggaatgtct
     1681 tcgcgccgtt cgcggatgct gggaaggttg atggaaatta cgttgagccg gtaatacaaa
     1741 tcttcgcgaa aagacccgtc cttgatcttt ttttcgagcg gttcgttggt ggcggcgagg
     1801 acgcgcacgt tgatgtaagt ggggatgttg tcacccacgc ggcggacttc gcgttcctgc
     1861 aacactcgca gcaagcggct ttggagcatc tgcgacatgg aaccgacttc atccaggaaa
     1921 atcgtgccac cgtccgcttc ctggaagagc ccttttttat cagccaccgc gccggtgaac
     1981 gagccgcgcc ggtgcccgaa caattcggat tcgagcaaat tttccggcaa cgcgctgcaa
     2041 ttgaccggga tgaacggcgc gaattgccgg cggctgttga aatgcaatgc gcgggcgacg
     2101 agttccttgc ccgtgccgct ttcgccgagg atgagaatgg tggagtcggt gtccgcaacg
     2161 cgttcaatca tcttgaacac gccttgcatt ggcgttgaag tgccgatgag ctggctgaac
     2221 tgatattttt tcttgagctg cttgcggaga taaagatttt ccgagatggc tacgttgtag
     2281 gaaagcgcgc gctggatgca aagattgagt tcatccagct tgaacggttt ttcaagataa
     2341 tcgtaagcgc cgcttttcat cgcctcaacg gcggtttcga ttgagccgaa cgcagtgatg
     2401 acaatcacgc tcgccggtga tggaagtgag cgggcccgct gcaggatttc gagaccgtgc
     2461 gcgcgggtct tgtcgagata aagatcggtg atgacgattt cgggcgcgtt ggcttccagg
     2521 gcggccatgg cgtccttgga attggtgaag gggaaaactt cgtgtccctc ctggcgaagc
     2581 atttcggtga ccatttgcac catggtcaac tcatcatcca caaggagaac ttttgccatg
     2641 ggaaaaggct aaggattttg gcgtgtgtcg gcaagcggga ttgttgaaca aatcaaaccg
     2701 gcttaacggt tggctcggat tcatgtttgc tcgtcatgaa ccggatgagc gcgagcatcc
     2761 cgatgacaag cagcaattcg aacaatgcgc cccaccaatg ctcgcgattg ttcggcagat
     2821 gatccagcca cgcgagcccc ggaatattgg caagaatcca cccgaggaaa tggtgcagat
     2881 gcggctgctg ataccatgaa agacgcaggc caaatatttc ctggccatgg atgaaaaccc
     2941 aatccagatc gggaagcata gcgaacaaaa ttccccattt gaacgagcgc caccagaaac
     3001 acaaaaataa aatcgtgaag accagcaccg tcagatggta actgtcccaa acaacactgt
     3061 ggaaatcagc ccggcgcggg tgataggtga cgttcgccaa tttgtccaaa atgccatggg
     3121 agagaaacgc gaggatggcc atcggcacaa acatgatctt gcggctccat tttctcagga
     3181 gggcgcgttg gaagatcact cccgccagaa tgtgagtcgg ttcctgcatc cctctatttc
     3241 atcccatcaa tcttcttcct ggtcgcttcg tccatgcgga tgagcggcgg ccagtcgcgc
     3301 gaaaatcctt cgccgggaag tttgtgcgtc gcgtcaatcc cgatcttgga cccggctgca
     3361 agctggctgg tggcgtggtc gagcacatcg gatggtcctt ttgtgaaaat gctgtcgcgt
     3421 tgcggatcgg tattcgcgca aagccggaac aaaacctcgc tggtgttgtg gacgttcaca
     3481 tccgcatcca cgaccacgat gtatttgctg aacatcattt gtcccatgcc ccagaggccg
     3541 tgcatgatct tgtaggcctg catcggatag gttttcttga tgctcacaaa gacgaggttg
     3601 tggaaaacgc cttccgccgg cagcgcgatg tccacaatct cgggaaaatt cattttgaag
     3661 atcggcagga acagtttcac gctcgcgctg ccgatgtaaa aatcttc
//
LOCUS       DASZMM010000435         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_477194, whole genome shotgun sequence.
ACCESSION   DASZMM010000435 DASZMM010000000
VERSION     DASZMM010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477194"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VG754_10460"
     CDS             <1..189
                     /locus_tag="VG754_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19682.1"
                     /translation="LSIMRMAQFVIWIWNANRPASQNGHFQKIACIAVSIAHNSSKGL
                     RTEQPGFCLERLILWSVL"
     gene            186..1715
                     /locus_tag="VG754_10465"
     CDS             186..1715
                     /locus_tag="VG754_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWF19683.1"
                     /translation="MKISNFYLPILAATTLAFAPLCASARDTEAVPTRTPGLDLAREL
                     NQAFVHVAEDVSPCVVVITVTEKASSTSHLDDSSDTEQDPLEPFRRFLHPQQDQQPQE
                     KTTGQGSGIIIRKDGYILTNRHVVEDAEKIEVRLKDGRTFRGEVRGEDAPADVAVIKI
                     EAKNLPVAKLADSSKTRVGEFAIAIGAPFALDYSVTFGHVSAKDRTDIIPNDGAQTSM
                     LDQSFIQTDANINPGNSGGPLVNIDGEVIGINTLIRGLHTGIGFAIPINLAREIADKL
                     ISDGKYSRSWLGVSIFSLREYPEFRSLVKGVQDGVVVHTIVPTGPAAQSNLKAADVIT
                     AVDGKPVVTSQQLKDEIRAKKAGQEVVLDVLRRTRVENSSNGSKHVMKALKITVRPLE
                     WVDQNMVASNEEKSPQESSANTNPLGIKVKALTSDMAKKYNVDLAEGVIVTYVERNSI
                     AAQSGIQEGDIITSIEEETVTNPKQFQEAIKGVDMKKGIIVHVISDGTAKFEILKEQA
                     D"
     gene            1901..2125
                     /locus_tag="VG754_10470"
     CDS             1901..2125
                     /locus_tag="VG754_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HWF19684.1"
                     /translation="MTRCRTLLEADLIVSRLAAAEIPAFIPDQFLLQAVAFNLNTYGY
                     VRVQVSPKDYEAAKEYLGNSEIDSPPKISP"
     gene            complement(2151..>2929)
                     /locus_tag="VG754_10475"
     CDS             complement(2151..>2929)
                     /locus_tag="VG754_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF19685.1"
                     /translation="SGLFQVSAVLGPAVGGFVMGRSPFWVYVVNIIGALLCALLIGLV
                     RVHHKVTVKEKMSLKSIATGLKFVYRTKVVLGSITLDLFAVLLGGATALFPVYANDIL
                     RIGPEGLGWLQAALPIGSLLMSVILVHRPPLQKAGRTLLWSVAGFGLATVGFGFAKSF
                     WFAFVMMFLCGVTDYISVVVRHTLVQLLTPDEMRGRVSAINSLFIGTSNQMGEFESGL
                     VAHWTNPVFAVVSGGIGTIVVVCVVATLWPEIRKYGRLDA"
BASE COUNT          761 a          900 c          709 g          559 t
ORIGIN      
        1 ctctccatta tgcgaatggc acaatttgtg atatggatat ggaatgcaaa caggccggct
       61 tcgcaaaacg ggcattttca aaaaatcgct tgtatcgccg tttctatcgc tcacaattca
      121 tcaaagggtc tcagaacaga acaacccgga ttctgcctcg aacgcttgat tttatggtcc
      181 gtattatgaa aatttcgaat ttctacttgc caattttggc ggccacgacg ctcgccttcg
      241 cgccgttatg cgcatccgcc cgcgataccg aagccgttcc cacccgcacg cccggcctcg
      301 accttgcccg cgaattgaac caggcatttg tccacgtggc tgaggacgtc tcgccctgcg
      361 tcgtggtcat cacggtgacg gaaaaggcca gttccacttc gcacctggac gacagttcgg
      421 atacagaaca ggatccgctc gaaccattcc gccgcttttt gcatccgcag caggaccagc
      481 agccccagga aaaaaccacc ggccaaggct ccggcatcat catccgcaag gacggctaca
      541 ttttgaccaa ccgccatgtc gtcgaggacg ccgagaaaat cgaagtccgc ctcaaggacg
      601 gtcggacatt ccgtggggaa gttcgcggcg aggacgcgcc cgcggatgtc gcggtcatca
      661 agattgaggc aaaaaatctt cccgtcgcaa aactcgccga ttcatccaag acgcgcgtcg
      721 gggaattcgc catcgccatc ggcgcgccgt tcgcgttgga ttacagcgtc accttcggcc
      781 atgtcagcgc caaggaccgc accgacatca tcccaaacga cggcgcacaa acttccatgc
      841 tggaccagag cttcatccag accgatgcca atatcaatcc cggcaacagt ggcgggccat
      901 tggtcaacat tgacggcgag gtcatcggca tcaacacact cattcgcggc ctgcacaccg
      961 gcatcggctt tgccatcccc atcaatcttg cgcgcgagat tgccgacaaa ttgatcagcg
     1021 acggcaaata ttcccgttca tggctcggcg tcagcatttt ttcattgcgc gaatatccgg
     1081 aattccgctc gctcgtgaaa ggcgtgcagg acggcgtcgt cgttcacacc attgttccca
     1141 ccggccccgc ggcgcaatcg aatttgaaag ccgccgacgt catcaccgcc gttgatggaa
     1201 aaccggtcgt gacttcgcag caattgaagg acgagatccg cgccaagaaa gccgggcagg
     1261 aagttgtgtt ggatgttctg cgcagaacgc gcgttgaaaa cagcagcaac ggcagcaagc
     1321 acgtgatgaa agcgctgaag atcaccgtgc gtccccttga atgggttgac cagaacatgg
     1381 tcgccagtaa cgaggaaaaa tctccgcagg aatcttccgc aaacaccaat ccgctcggca
     1441 tcaaggtcaa ggcgctcacg tctgacatgg cgaaaaagta caacgtggac ttggccgaag
     1501 gcgtgatcgt gacgtacgtg gaaagaaaca gcatcgccgc gcaaagcggg atacaggaag
     1561 gcgacatcat cacctcaatc gaggaggaaa ccgtcaccaa tcccaaacaa ttccaggaag
     1621 ccatcaaagg cgtggacatg aaaaaaggca tcatcgtcca cgtcatcagc gacggcaccg
     1681 caaaatttga gatactgaaa gagcaagccg attaaccatg aaacattgcg attattgcgg
     1741 acacgacaat ccggacgatg cgattgtttg tgccggatgc ggcaccgacc aatttaaaac
     1801 ggaatcaacg agcgtcgcag cagactccaa gaattttcct gcaactccaa ttcactttgt
     1861 tccgcttgcg tcaggcgata tggacaagga cttggcgacc ttgacgaggt gcagaacact
     1921 tttggaagca gaccttattg tcagccggct tgctgccgct gaaatacccg ctttcatacc
     1981 cgatcaattt ttgcttcaag ccgtcgcttt taacctgaac acgtatggtt atgtaagggt
     2041 gcaggtttct cccaaggatt acgaggcggc aaaagaatac ctcggcaact ccgaaattga
     2101 ttcaccccca aagatttcgc catgacgtaa agccatgatc gataggccgt ttacgcgtcc
     2161 aaccggccgt actttcttat ttccggccaa agcgtcgcca ccacgcaaac aacaacgata
     2221 gttccaatcc cgcccgaaac gaccgcgaaa accgggttcg tccaatgcgc caccaatccc
     2281 gattcgaatt cacccatctg gttcgacgtg ccgatgaaca ggctgttgat cgccgaaacg
     2341 cgcccgcgca tctcgtcggg cgtcagcaat tgcacgagcg tgtgccgcac cacgacactg
     2401 atgtaatccg tcacgccgca gaggaacatc atcacgaacg cgaaccaaaa acttttggca
     2461 aaaccgaacc caactgtcgc cagtccaaat ccggcgacgg accaaagcaa tgtccgcccc
     2521 gccttctgca acggcggccg atgcaccaat atcaccgaca tcaacaacga ccctatcggc
     2581 agtgccgcct gcaaccagcc caaaccttcc ggcccaatcc gcaaaatgtc attcgcatac
     2641 acgggaaaca aagccgtcgc gccacccaac aataccgcga acagatccag cgtgatgctt
     2701 ccgagaacga ccttcgtgcg atatacaaac ttgagtccgg tcgcgatgct tttcaacgac
     2761 atcttctcct tgaccgtgac cttgtgatgc acccgcacca acccgatcaa aagagcgcac
     2821 agcagcgcgc caatgatgtt cacgacataa acccaaaatg gggaacgtcc catcacgaac
     2881 cctcccactg ccggtcccag caccgccgat acctggaaca atccgctgt
//
LOCUS       DASZMM010000436        10810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2103799, whole genome shotgun sequence.
ACCESSION   DASZMM010000436 DASZMM010000000
VERSION     DASZMM010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2103799"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VG754_10480"
     CDS             <1..710
                     /locus_tag="VG754_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWF19686.1"
                     /translation="IGLALTLGLLFYFEGLMTSRYLPMLLLGLVLTGAAVIPFSNSLP
                     LSVQRSLTFLPLNLDPEAVRSAEDSTNWRLEMWKDVLPQVPQYLVLGKGLKMDAADLA
                     MLSSGMNAGGIGAAGSELAGDYHSGPLSLIIPFGLFGVAGFIWFYVAGFCVLHRNYKF
                     GDPIYERINRFLLVSYLIRIFTFLFVFGSFYSDMLFFIGPIALSIALNGGVRKAVEEK
                     PDVKPAMGRLKLAGATR"
     gene            788..1618
                     /locus_tag="VG754_10485"
     CDS             788..1618
                     /locus_tag="VG754_10485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF19687.1"
                     /translation="MRFSVITPSFRNSNWLRLCIASVADQGVACEHIIQDAGSDDGTL
                     DWLRQDSRVKAFIEKDSGMYDAVNRGLRRAEGDILSYLNCDEQYLPGALAAVEKFFEE
                     NPRVEVLFADFVVVDTEGKYRFHRKVQTPQLYHTWVSHLPAFTCATFFRRSVIAERGL
                     YFNPKLRVVGDADWMLRLLRNRVPMAVLRRFTSAFTLTGENLGTKPEGLREAAELFRS
                     APLWARKSKPLLVLQHRLRRLAGGIYFQRPFSYSVYSLNDPKVRTAHQVTMPDFRWKS
                     "
     gene            1740..2765
                     /locus_tag="VG754_10490"
     CDS             1740..2765
                     /locus_tag="VG754_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF19688.1"
                     /translation="MKPKLLIVELWGLGDLAIATPFLRKACERFEVTLLAKPFAHDLQ
                     SRFWPQVKVIPFNAPWTAFAFGRKYQLFSWPWRTIFSVWKKLFDEHFDVALSARWDPR
                     DHFLLNVSRAKTRLGFPRVGSQIFLTNPLAAPDHAAHRYESWLTLARALNLNLETRNK
                     LSFPEPRGKKLILVHTGAAQPVRVWPLDQYCQLVKRLRKKGHAVKVICNPEQQGWWKR
                     AGETEAIAPQTVSELLHCLDEACAFIGNDSGPGHLAAVCGIPTFTFFGPQIVEWFVPL
                     HPAAEFAEGKACPYKPCSDYCRFPVPHCLWNITEEEIWPKVEKFVERHLAQDSQKSDR
                     FTREPVT"
     gene            complement(2758..4650)
                     /locus_tag="VG754_10495"
     CDS             complement(2758..4650)
                     /locus_tag="VG754_10495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19689.1"
                     /translation="MSKRLKVLLSAYACEPNKGSEPEVGWQWAMQMAHYHDVTVLTRA
                     NNREAIEKGLESLRDKQPLPRFVYHDEGPFLLWLKNEFHTIRFYYIFWQISAWKIISR
                     LNKENRFDLLHHVTFAGFRYRTAIWNHGVPTVWGPVGGIQSIPWRLLPWKYPSALMLE
                     VFRHFNNFVQSAPFHFLPKRAQMTTMTLVSTREMEATFLRLGLETTLMPTIGLNTREL
                     SVPSRQLTKGPLKVLFVGSVITLKGIDLAIHAMAEAGIDAVFTIIGSGKFEAMARRLV
                     KQLGVESKVEFRGRLSLRETLGSYADYDLFLFPSLHDTGGYALIEAMFSGLPAICLDC
                     GGPRLAVKEGTGIKVPLGSRKAVLTGLAEAIRHYDSDRQLLLEHGRAAHETIVRDYDW
                     ERKGRELDAIYQKAATGGPEVPKGVAQSLPLKNLFAGVLSIKALVFSAAILFIVATAG
                     FLSVELLKRNAELIVKDTLPGLSDAGAVNSTMAQAFNRTVLLMMSAKPEERAAYRREL
                     DAFTQQTGESLSLYQNTKFTGPEHALYTEVLKRRQNYQLARQQVLDLLDHQKQAEAVQ
                     ACKTSMLPAYLEYKTAAEKLLNYDTRIGRSRGESILKICLITQCAVAAIGAALFVAGF
                     IIGFFK"
     gene            complement(4668..5849)
                     /locus_tag="VG754_10500"
     CDS             complement(4668..5849)
                     /locus_tag="VG754_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19690.1"
                     /translation="MNTRKDFLQMLFRSLDANGISYCVLRNYEGLLEEGVTADIDLLV
                     ERRDLDRFSETLRTAASESGHRFVHHARYVNHSCVFWSPQSNFTRVDFDTEIRWRLFP
                     ILSARAILEARQRCGEFYIPHPRHESVVLFAQALWMEKLDERYRVQLARLYDVCENEL
                     LTRTYRKAFGSAGNVLEEFHAQVLKREFTKSFCSSLKRSLIFKTFAHPSTGWPDFAGN
                     VIDDGFRLWERLRRPAGISLLYVTSVGSKKNLDALMHRVEFLFPAQKCFQHAVDLTQG
                     RKPVRWGMSLELRRLRALFKGGLCVQFYQLAQGADISAVTKAASHRLYTARCFICTED
                     SNGRACLTHVGTGFQAGTADVEKSMKTDFSALLIGFISTVLEKENRRGIFGRKLARTA
                     L"
     gene            complement(5846..7117)
                     /locus_tag="VG754_10505"
     CDS             complement(5846..7117)
                     /locus_tag="VG754_10505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF19691.1"
                     /translation="MHETTGHPAVMSEKNVKPRVLMIAYACNPRGSGEHWLGWGWAEQ
                     ASRAYQVDLITTPNNRQAVEERARDCGINVHFVGLPHWLRTVTEFFRGGAWLRQTWWQ
                     FRIAKLAKSLHQQKSFALVHQTTFHSFRIPFLTANTLDIPSVWGPIAGGESVPPGFSR
                     YLGSARFFESFRTAANRVWLQFPPVKKSLRRASAIFVSNRTTLVFLPAEAREKCRIVP
                     PNALRPEDEHPPSEPPVPTKSQTLRLLYVGNCVPTRAIPLLFDALIESGLKDYKLTIV
                     GSGPAQPDWMRKAAELGIKVEFTGQVSREKLPGYYTASDALVFPALRDSGGSALLEAM
                     SRYLPVICLDWAGPGEMVDESGGIKVPVDPPGKTVKAFAAALVRIKEDPALRVSLARS
                     AQKRAQTIFRWDEKFRLLQTTYDRLIETKVA"
     gene            complement(7110..8435)
                     /locus_tag="VG754_10510"
     CDS             complement(7110..8435)
                     /locus_tag="VG754_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF19692.1"
                     /translation="MQEHPVVIIGAGLTGLSCAHHLGGNYLLVEKELEPGGIVRTRIR
                     QNSFYCDGTGHWLHLRNAAMKDLVNKLLAGNLVEHERKAVIFSHGVFTAYPFQANTYG
                     LPREVIMECLLGLLKAKHPEDFGQKPIAEPPNNFHEWIVRAFGEGIARHFMVPYNQKL
                     LGVKLTELMPAFAERFIPRPSTEDVIKGALGFSKESLGYNAKFVYPKEGGIGALSRAF
                     ANSLKEPPLYGVHVTSVDLKQKTVSLSNGKTMRFGRLVNTMPLVQLISLLKDAPDEVR
                     VAAKNLRATTVYYYDIGVRGKGGPASHYHWVYFPEPEFIFYRAGSYSAVHKEAAPPGC
                     RSYYVEMSGDLEGLRLQPEKLKQRVLIDLKKAGVLAVDDEILFAELCEIPHAYVIFDQ
                     HYEESRGLLFDYLKNNNVLTGGRWGGWGYGGMEDAMLDGKAAAEQIHNA"
     gene            complement(8442..9191)
                     /locus_tag="VG754_10515"
     CDS             complement(8442..9191)
                     /locus_tag="VG754_10515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWF19693.1"
                     /translation="MMLVFTYHEVRDCAGEGETGFYAISPAQLARHLEILDAKGLGFL
                     KLEELENNSPENKCILTFDDATCDHYDVVFPMLEKHRWRGSFFVPTAKLNQPGYLTDA
                     QVREMSAAGHEIGSHSHEHQRMDRLSEEEVRQQMTRSQEILAGIIGKKPVTFVPPGGF
                     MNEHIRKVAGELGFRGIRTMRWGYNQKMDLMGLETLPINHYTGEKRFQKLLEPQGASA
                     LYAGKEMLKKLMPLRNYEKLRKLAFKFWKFN"
     gene            complement(9188..10108)
                     /locus_tag="VG754_10520"
     CDS             complement(9188..10108)
                     /locus_tag="VG754_10520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF19694.1"
                     /translation="MNWLVKAAAFKLLSALPGGPALYRYGQEHLTHSLVPTPERVGQK
                     IDVGLQYWDWLAAHQPHKKPLGTHLDFGAGWHPTIPLLFYSLGVEQQHLVDVAPLLDR
                     GLIESTVKAFLKIVTEPGWAHRSRLQRLPDSVWDNEMPWTAYLKCLGVTCHAPFTGAE
                     PELVGAIDFATSTQVLYFIPREPLRSCFRQLHRVLKTGGNFLATIHLNDVNATWQKGL
                     SQYNHLQYSPDVWDKWINSQLMSYNRLKAADYRQLLEEAGFQILHFDVTGPTAADLEE
                     LSRIPIHNSFSRYSREELGAKHLFFVAQKV"
     gene            complement(10133..>10810)
                     /locus_tag="VG754_10525"
     CDS             complement(10133..>10810)
                     /locus_tag="VG754_10525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF19695.1"
                     /translation="VKAFIEKDKGMYDAVNRGWKRAQGDIIAYLNCDEQYLPGALKAV
                     GDFFQSHPDVDVVLPDTIVVDDKGEYFCHRPSLVPLKHQIWIRFSVLTCSVFIRRRVV
                     EKMGLYFDTKWRDLGDVFWFMELVNRGVRMAVLPQFASVFTETGDNMNLKPNALREKA
                     EKVEMTPAWIRRLSKLMILHHRWRMFARGAYFQRPFDYSIYTLANPAQRVTHHVAKPT
                     ARWRGRS"
BASE COUNT         2444 a         2968 c         2878 g         2520 t
ORIGIN      
        1 tgataggcct ggcgcttacc ttaggcctgc tgttttattt cgaaggcttg atgaccagcc
       61 gctatctgcc catgcttttg ctcgggttgg ttttgacggg cgcggccgtc attccgttct
      121 cgaacagttt accgctctca gtccaacgtt ctctcacatt tcttccgctg aacctcgatc
      181 ctgaagcggt gcgcagcgcc gaggactcca ccaactggcg gcttgagatg tggaaggatg
      241 ttctgccgca ggtgcctcaa tacttggttt taggcaaggg cctgaagatg gatgccgcag
      301 atttggcaat gctttccagc ggcatgaatg ccggcggcat cggagcggct gggtctgagt
      361 tggctgggga ttatcacagt gggccgctct ctctgatcat tccatttggt ctcttcggag
      421 ttgcgggatt tatctggttc tacgtggccg gtttttgtgt gctgcaccgg aactataagt
      481 ttggcgatcc gatttatgaa aggataaatc ggttcctgct ggtcagctat ctcataagaa
      541 tcttcacgtt cctgtttgtt ttcggctcgt tttactcaga catgctgttt tttatcgggc
      601 ctattgcatt gagcatcgcg ttgaacggcg gcgtacgcaa ggcggttgaa gaaaagccgg
      661 acgtaaaacc cgccatgggc cgtctcaaac tggcaggcgc aacgcgatga gcccctcaac
      721 aatggcggct ttgtggggaa cttgggcaga acgggccgcc ggtgactttt cctgactccg
      781 ccagatcgtg cgcttctcgg tcatcactcc cagcttccgc aattcaaact ggctgaggct
      841 ttgcatcgcg tccgttgctg atcaaggtgt cgcctgcgag catatcatac aggacgcggg
      901 ctctgacgat ggcacgctcg attggctgcg gcaagattcg cgagtgaagg cattcatcga
      961 gaaggattcg gggatgtacg acgccgtgaa ccgtggcttg cgtcgcgctg aaggcgatat
     1021 tttgtcttac ttgaattgcg atgaacaata ccttccaggc gcgctcgccg cggtggagaa
     1081 attttttgag gagaatccgc gagttgaggt gttgtttgct gattttgtgg tggtggatac
     1141 ggaaggaaag tatcgtttcc atcgcaaggt ccagacgccg caactttatc acacctgggt
     1201 ttcacatctg ccggcgttca cttgcgccac tttttttcgg cgcagtgtca ttgcggagcg
     1261 tggtctttat tttaatccca agctccgggt ggtgggcgat gcggattgga tgctgcggct
     1321 gttgcgcaat cgcgtcccga tggcggttct tcgcaggttc acttcggcgt tcacgctgac
     1381 tggcgagaat ttgggaacaa aaccggaggg cctgcgcgag gctgctgaat tgttccgttc
     1441 cgcgccgttg tgggcgagaa aatcaaaacc gttgctcgtc ttgcaacatc ggctgcgccg
     1501 cctggccgga ggcatttatt ttcagaggcc attttcatac tcagtttata gtttgaatga
     1561 tccgaaagtc cgaacagccc atcaagtcac catgcctgat ttccgctgga aatcatgaaa
     1621 atgaccaaat ggccgagctg tccatcatca gttcgcttct ttacagtctg atggaacggc
     1681 agtaaaatac agccgaagca atcaatgttt tccagtcttc aacctgcatt ccgcccctcg
     1741 tgaaacccaa gctgctgatc gtcgaattat ggggcttggg ggaccttgcc atcgccacgc
     1801 cgttcctgcg caaggcctgc gaacgattcg aagtcactct cctggcgaag ccctttgcgc
     1861 acgatttgca atctcgcttt tggccgcaag tcaaagtgat accgttcaac gctccgtgga
     1921 ccgccttcgc tttcggccgc aaatatcaac tgttctcatg gccgtggcgc acgatatttt
     1981 ccgtctggaa aaaactgttc gacgaacatt ttgatgtggc gctttccgcg cgttgggacc
     2041 cgcgcgacca ttttcttttg aacgtctcga gggccaaaac ccgtttggga tttccacgcg
     2101 tcggcagcca gatttttctc acgaatcctc tggctgcgcc cgaccatgcg gctcaccgct
     2161 acgagagctg gctcaccctc gcgcgggcgt tgaacctgaa tttggaaact cgcaacaagc
     2221 tctcctttcc cgagccccgc ggcaaaaagc tgatcttggt gcataccggc gctgcgcaac
     2281 ctgtccgcgt ctggccgctc gaccagtatt gccaattggt gaaacgactc cgcaaaaaag
     2341 gccatgccgt caaggtaatc tgcaatccgg agcagcaagg ctggtggaag cgggcgggcg
     2401 aaaccgaggc catcgctccc caaaccgtca gtgaattgct gcattgcctg gatgaagcct
     2461 gtgcgttcat cggcaacgat tccgggccgg ggcatcttgc ggcggtttgc ggcattccta
     2521 cttttacttt tttcggtccg caaatcgttg aatggttcgt tccgcttcat cccgcggcag
     2581 aattcgcgga aggcaaggct tgcccataca agccttgctc ggattattgc cgttttccgg
     2641 tcccccattg cctctggaac atcacagagg aggagatctg gccgaaagtg gagaagttcg
     2701 tggaacggca tctggcccaa gactcacaaa aaagtgaccg ctttacccgt gagcccgtca
     2761 cttgaagaaa ccgatgataa aacccgccac gaacaaagcc gcgcctatgg cggcgacagc
     2821 gcattgagtg atcaggcaga ttttgaggat ggattcgccg cgtgacctgc ctatgcgagt
     2881 gtcgtaattc aacaattttt ccgccgctgt tttgtattcc aagtaagctg gcaacatgga
     2941 agttttgcac gcctgcacgg cttccgcttg cttctgatga tcaagcaaat cgagcacctg
     3001 ttgacgggca agctgatagt tctggcgtct tttgagaacc tccgtataaa gcgcatgttc
     3061 cgggccggtg aacttggtgt tctggtagag acttaaggac tcgccggtct gctgcgtgaa
     3121 tgcatccagt tccctgcgat aagcggcgcg ttcttcgggc tttgccgaca tcatcaaaag
     3181 tacagtgcgg ttgaaggcct gagccatggt ggagttgacc gcgccggcgt cagagaggcc
     3241 gggtaatgtg tctttgacaa tcaattcggc attcctttta agaagctcca cactcaggaa
     3301 accagccgtg gcgacaatga ataaaatcgc agcggaaaaa accagcgctt tgatggatag
     3361 aaccccggca aacaaatttt tcaatgggag cgattgggcc acgcccttgg gcacttcggg
     3421 cccgccggtc gcggctttct gatagatggc atccagttcg cggccttttc tttcccaatc
     3481 gtagtcgcgc acgatggtct cgtgcgctgc gcggccatgc tcaagcaaga gttgccgatc
     3541 gctatcataa tgccggatgg cttcggccaa cccggtcaac acggctttgc gagaacccaa
     3601 gggaaccttg attccagttc cttctttcac agcaaggcga ggccctccac aatcaaggca
     3661 gattgccggc aagcctgaga acatcgcctc aatcaacgca tatccgccgg tgtcatgcag
     3721 acttggaaaa aggaacaaat cataatcggc atacgaaccg agcgtttccc gcagtgaaag
     3781 ccgtccgcga aattcaacct tcgattcaac cccaagttgc ttgacgagcc ttctggccat
     3841 cgcctcaaac ttgccgcttc cgatgatggt gaaaactgca tcaattcccg cttccgccat
     3901 cgcatggatg gcaaggtcaa tgcccttgag cgtgatgacg ctgcctacaa aaagcacttt
     3961 caatggaccc ttggtcaact ggcgcgacgg aacagatagt tctctggtgt tcagcccgat
     4021 ggtcggcatc aacgtcgttt ccaagccgag gcgaaggaag gttgcttcca tttcccgtgt
     4081 tgaaacaaga gtcatggtcg tcatctgcgc ccgcttcggc aaaaaatgaa acggggcgga
     4141 ctggacgaaa ttattgaaat gcctgaagac ttccagcatc aatgctgaag ggtatttcca
     4201 gggaagcaac cgccatggga tggattgaat cccacccacc ggcccccaga ccgtcggcac
     4261 tccatggttc cagattgcgg tgcgatagcg gaagccggca aaggtgacgt gatgcaagag
     4321 atcaaagcgg ttttccttgt tcaatcggga gatgattttc caagccgaaa tctgccagaa
     4381 aatgtaatag aaccggatgg tatgaaactc gttcttaagc catagcagga aaggtccttc
     4441 atcatgataa acaaaacgag gcagcggctg tttgtcgcga agtgattcaa ggcctttttc
     4501 aatggcctcg cgattgtttg cgcgggtcag gacggtgaca tcatggtagt gggccatctg
     4561 catggcccat tgccatccga cttccggttc ggaccctttg ttaggctcgc aggcgtacgc
     4621 tgagagcagc acttttaatc ttttagacat gcaacaaaca atctttctta cagcgctgta
     4681 cgagccaatt tccttccgaa aattccacgg cggttttcct tttccaagac cgtggatatg
     4741 aaacctatca acagcgcgct gaaatcggtt ttcatggatt tttcaacatc ggcagtgccg
     4801 gcttgaaacc ctgtcccgac gtgcgtgaga caggcccggc cgttggaatc ttccgtgcaa
     4861 ataaaacatc tcgctgtata aagacggtgc gaagccgctt tagttacagc cgatatgtcc
     4921 gcaccttgtg caagctggta aaactgcacg cacaatccac ccttgaagag cgcgcgcaaa
     4981 cgccgcagtt cgagactcat cccccacctc acgggtttcc taccctgtgt caaatcaact
     5041 gcatgctgaa aacatttttg ggccgggaac aagaattcta cccggtgcat caaggcatcc
     5101 agattttttt tcgatccgac ggaagtaacg tataacaggg aaatgcccgc cggtcgccgc
     5161 agacgttccc aaaggcggaa tccgtcgtca atcacattgc cggcaaaatc cggccaccct
     5221 gtcgaaggat gcgcaaatgt cttaaaaatc agggagcgct tgagtgaaga acagaatgat
     5281 ttggtgaact cacgctttaa aacttgggca tgaaactcct ctagcacgtt tcctgcgctc
     5341 ccgaaagctt tgcgatatgt ccgggtgaga agctcgttct cgcagacatc gtacagcctc
     5401 gccaactgga cgcgataccg ctcgtccaat ttctccatcc acaatgcctg cgcgaacagc
     5461 acaactgatt catgtcgcgg atgcggtatg taaaattccc cacatctctg cctcgcctcc
     5521 aggatggcgc gggcggaaag gatgggaaac aatcgccagc ggatttccgt gtcgaaatca
     5581 accctggtga agttgctttg gggactccaa aaaacgcagg aatgattgac gtatcgggca
     5641 tgatgcacga aacggtgccc cgattcgctc gccgcggtcc gaagcgtctc gctaaatctg
     5701 tccaagtccc gccgctccac gagtaaatcg atgtctgccg tcaccccctc ttccagcagc
     5761 ccctcatagt tgcgcaacac gcaatagctg attccattcg catccagcga acggaacagc
     5821 atttgcaaaa aatctttgcg cgtgttcatg cgaccttggt ttcaataagc cggtcgtaag
     5881 tggtttgcag caaacgaaat ttttcatccc aacggaaaat tgtttgtgcg cgtttctgcg
     5941 ccgagcgcgc aagtgaaaca cggagcgcag ggtcttcctt gatccgcacc aacgccgccg
     6001 caaaggcttt gacggttttc ccgggcggat ccaccggcac ttttattcct ccgctttcat
     6061 ccaccatttc gccgggaccg gcccaatcca ggcagatgac cggcaggtac cgggacatgg
     6121 cttccagcaa ggccgagccg ccgctgtccc gcagcgcggg gaaaaccaac gcatcggaag
     6181 cggtgtaata accgggcagt ttttcgcgcg atacctgccc ggtgaattca accttgattc
     6241 caagttccgc ggcctttcgc atccaatccg gctgcgccgg tccgctgcct acgattgtga
     6301 gtttgtaatc cttcaaaccc gactcgatca atgcatcgaa caacagcgga atggcgcgtg
     6361 ttggcacgca attgccgacg taaagcaagc gcaatgtctg actttttgtc ggaacaggcg
     6421 gttcggacgg cggatgttca tcttccggtc gcaatgcgtt cggcggaacg atgcggcatt
     6481 tttcccgcgc ctcggctgga agaaaaacaa gtgtggtgcg attggaaaca aagatggctg
     6541 aagcgcgacg gagtgacttt ttgacgggcg gaaattgcag ccagacgcgg ttcgccgccg
     6601 ttcggaaaga ttcaaaaaac cttgccgagc ccaaataccg ggaaaagccg ggcggaacgg
     6661 actctccacc cgcgatcggg ccccagacgg aggggatgtc gagcgtgttg gccgtgagga
     6721 atggaatgcg aaaagaatga aaggtcgttt gatgtaccag cgcaaaagac ttctgctgat
     6781 gaagggattt tgccagcttc gctatgcgaa attgccacca cgtttgtcgc agccatgcgc
     6841 cgccgcgaaa gaattcggtg accgtcctga gccagtgcgg cagcccgacg aaatgcacgt
     6901 ttataccgca atctcgcgcg cgttcttcca ccgcttgccg gttgttgggc gtggtgatca
     6961 aatccacctg atacgcgcgc gaagcctgtt ccgcccaccc ccatcccagc caatgctcgc
     7021 cactgccccg cggattgcac gcgtaagcga tcatgagaac acgcggtttt acgttcttct
     7081 cggacatgac ggcgggatgg ccggttgtct catgcattgt ggatttgctc cgcggccgcc
     7141 ttgccatcga gcatggcgtc ttccatgccg ccgtaacccc acccgcccca gcgcccgccg
     7201 gtcaggacat tgttgttctt caaataatcg aaaagcaaac cgcgcgattc ttcgtaatgt
     7261 tggtcaaaaa taacgtacgc atggggtatc tcgcataatt cggcgaagag gatttcatca
     7321 tcaacggcca ggacacccgc tttttttaga tcaatcagca cgcgttgttt caatttttcc
     7381 ggctgcaagc gcaacccttc caaatcgccg gacatctcca cgtaataact gcggcaaccc
     7441 ggcggcgcgg cttccttgtg cacggcggaa tacgaacccg cgcgatagaa aatgaattca
     7501 ggttccggaa aatagaccca atgataatgg ctcgcgggtc cgcctttgcc ccgaacaccg
     7561 atatcgtaat aataaaccgt cgtggcgcgg agattttttg cggcgacgcg gacttcgtcc
     7621 ggcgcgtcct tcaatagcga aatcaattgg accaacggca tggtgttgac caatcttccg
     7681 aagcgcatag tttttccatt ggaaagtgaa actgtttttt gcttgaggtc cacactggtc
     7741 acatggacgc catacaacgg cggctctttt agagaattgg cgaaggcgcg cgacaatgcg
     7801 ccgatgcctc cttccttggg ataaacaaat ttcgcattat acccgagcga ttccttggaa
     7861 aaacccaacg cgcctttaat gacgtcctcc gtggagggac gcggaataaa acgttccgcg
     7921 aacgccggca tcagttccgt gagtttgacg cccaggagtt tttgattgta cggcaccatg
     7981 aaatggcggg cgatgccttc gccgaacgcg cgcacgatcc attcatgaaa attgttggga
     8041 ggttccgcga taggtttctg gccgaaatcc tcgggatgct ttgccttgag cagcccaagc
     8101 aggcattcca tgatgacttc gcgcggcaaa ccgtaggtat tcgcctggaa aggataagcc
     8161 gtgaacacgc catgggaaaa aatgaccgcc ttgcgctcat gctccacaag attgccggcc
     8221 agcagcttgt tgaccaagtc cttcatcgcg gcgttgcgca ggtgcagcca atggccggtg
     8281 ccgtcgcaat aaaatgagtt ttgccggatg cgtgtgcgca cgatgccgcc cggttcgagt
     8341 tccttttcga ccagcagata gttcccgccg agatgatggg cgcaagaaag tccggtcaat
     8401 cccgcgccga tgattactac tgggtgttct tgcatttcag ctcaattgaa tttccaaaat
     8461 ttaaaggcca gcttgcgaag cttttcgtaa tttcgcagcg gcatcaattt cttcagcatc
     8521 tccttgccgg cgtaaagcgc cgatgcgccc tgcggctcaa ggagtttctg gaatcttttt
     8581 tcgccagtat agtgattgat cgggagggtt tccagcccca tcaaatccat tttttgattg
     8641 tagccccaac gcattgtgcg gatgccccga aagccgagtt cgcccgcaac cttgcggatg
     8701 tgctcgttca tgaatccgcc gggcggcacg aacgtgaccg gtttctttcc gatgatgccc
     8761 gcgaggattt cttgcgagcg ggtcatctgc tggcgaacct cttcttcgga caacctgtcc
     8821 attcgctgat gttcatggct gtgcgaaccg atttcgtgac cggcggctga catttcgcgc
     8881 acttgcgcgt cggtcaggta acccggttgg ttcaatttcg ccgtgggaac aaaaaaagag
     8941 ccacgccacc gatgcttttc caacatgggg aacacgacgt cgtagtgatc gcacgtggcg
     9001 tcgtcgaagg tcaggatgca tttgttttcc ggggagttat tttccaactc ttccaatttc
     9061 aaaaacccca aaccttttgc gtcgagtatt tcgagatgac gggccagttg cgccggggaa
     9121 atggcataga agccggtttc gccctcgcca gcgcaatcgc gaacttcatg ataggtaaaa
     9181 acgagcatca taccttttgc gcgacgaaaa agagatgctt cgctccgagt tcttctcgcg
     9241 aataacggct gaaagaattg tgaatgggaa ttcgtgatag ttcctccaag tccgcagccg
     9301 tcgggcctgt cacgtcaaaa tgaaggattt ggaagccggc ctcttcgagc agttggcgat
     9361 aatccgcggc tttcaagcgg ttgtacgaca tgagctggga attgatccat ttgtcccaaa
     9421 cgtccggcga atactgcaaa tggttgtatt gggaaaggcc cttttgccac gtcgcgttca
     9481 catcattcaa atggatcgtc gccaaaaaat tgcctccggt tttcaaaacg cggtgcaact
     9541 ggcgaaagca actcctcaat ggctcacggg gaatgaaata aagcacttgg gtggaagtgg
     9601 cgaagtcaat tgccccgacc agttccggct ctgctcctgt aaatggagca tggcacgtca
     9661 ctcccaagca cttcaaataa gcggtccatg gcatttcatt gtcccaaacg ctatcgggaa
     9721 gccgctgcag acgcgaacga tgcgcccaac cgggctcggt cacgattttg agaaatgcct
     9781 tcactgtgga ttctatcaat ccgcgatcca gcaacggagc cacatcaacc agatgctgtt
     9841 gctcgacgcc cagcgagtaa aaaagcaaag gaatggttgg atgccagcca gccccaaaat
     9901 ccaggtgagt cccaagcggt ttcttgtgcg gctgatgggc agcaagccag tcccaatatt
     9961 gcaggccgac atcaatcttt tgcccgactc gttccggggt tgggacgagc gaatgggtca
    10021 gatgttcctg gccgtaacgg taaagcgcag gaccgcccgg aagtgctgat agcagcttga
    10081 acgcggcagc tttgaccaac cagttcatag taataaaacg ttggactgaa actcaggacc
    10141 ttccccgcca gcgcgccgtt ggtttggcca catggtgcgt gacgcgctga gcaggattgg
    10201 caagtgtata aatcgaataa tcaaatggcc gctgaaaata agcgcctctt gcgaacatgc
    10261 gccagcggtg atgcaaaatc atcaatttcg aaagccgcct gatccacgcc ggtgtcattt
    10321 cgactttttc cgccttctcg cgaagcgcgt tgggtttgag attcatgttg tcgccggtct
    10381 cagtgaagac agaggcgaac tgcggcagca ccgccatgcg caccccgcga ttgaccagtt
    10441 ccatgaacca gaaaacgtcg ccgaggtccc gccacttcgt gtcgaaataa agtcccattt
    10501 tctccactac acgtctccgg atgaaaaccg aacaggtcaa cactgaaaac cggatccaaa
    10561 tctggtgctt caacggaaca agtgacggcc gatgacaaaa atattctccc ttatcatcca
    10621 cgacgatggt gtccggcaac accacgtcaa cgtccgggtg cgattggaaa aagtcgccca
    10681 cggctttgag cgcgccgggg agatattgtt cgtcacaatt gaggtaggcg atgatgtcac
    10741 cttgcgcgcg cttccaaccc cggttcactg cgtcatacat ccctttgtcc ttttcgatga
    10801 aagccttgac
//
LOCUS       DASZMM010000437         7857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4587519, whole genome shotgun sequence.
ACCESSION   DASZMM010000437 DASZMM010000000
VERSION     DASZMM010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4587519"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1368
                     /locus_tag="VG754_10530"
     CDS             22..1368
                     /locus_tag="VG754_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HWF19696.1"
                     /translation="MSTTPPTDALPKSPPLEKQTTVGNYFVSNYPPFSFWKQELVTDL
                     YAALERPHKPGNPLGIYLHIPFCRKRCHFCYFRVYTDKDSAAIKSYIDASLQELKIYA
                     GKPAIRGRKPNFIYFGGGTPSYLSVDQLKHLFSGMKEMLPWDEAEEVAFEAEPGTLTD
                     QKLKAIRDLGVTRLSLGVENFDDHILEINGRAHHSKEIARAYGYARELNFPQINIDLI
                     AGMVEETEANWRECVRKTIEMSPDSVTVYEMEIPYNTTIYKQMKAEGKLVAPVADWET
                     KRAWVNYAFNELEKAGYTISSAYTAIKNPQRTKFIYRDRLWAGADLLSLGVASFGHIN
                     GTHYQNHHDFDPYLTRVQQGELPVYRALTPNDDERLIREFVLQLKLGHVSRNYFKQKF
                     GVEPAERFAAPLQTLKDWGFLTIDDDTVRLNREGLLQVDRLLQEFFLPQHKNVRYA"
     gene            1361..1945
                     /locus_tag="VG754_10535"
     CDS             1361..1945
                     /locus_tag="VG754_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19697.1"
                     /translation="MPERASTIPASTTTNFSLAHPLDEFYAEEGQPVPPLNQIDGEAV
                     PEPYRTLLVHQNDMTPTLEAFHRSSVHLDVLGRRRKGDAYFREVILRLDSNDQPVEFG
                     AIKINLALFTPEAREYILHEKLPLGHILHQYKIAHASRPRAYLRLASDKLISKVLNLN
                     GAQVLYGRRNTLYDPQQRPLAEIVEILPVAATRH"
     gene            2007..3287
                     /locus_tag="VG754_10540"
     CDS             2007..3287
                     /locus_tag="VG754_10540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF19698.1"
                     /translation="MNSNSKSDILIIGAGPAGSTAAALLAEKGHRVLVLEREKFPRYH
                     IGESLLPFTYQPLERLGLIDRMRKSAFVKKYSVQFVSPSGRASLPFYFFNRYDRESVA
                     QTWQVLRSEFDQMLLDNARAKGAEVKEEVTVKELIREHGRVVGARSQLKDGTPTDFRA
                     TITLDCTGKEAFTAVRNGWRMKDPYLNKIAVWTYYKGSKRDEGVDEGATTVAYVPEKG
                     WFWYIPQHNDMISVGVVAEGKYLTRDGVKSPEDIFKREIEQNLWIKEHLACGEQTGSY
                     FLTSEYSHHSKHCATDGLLLVGDAFAFLDPVFSSGVMLALKSGVLAADAVHQAFVEKD
                     FSPERFSDYASFLRQGVENMRKLVYAFYDPNFSFGKVIKKHPDAAGLITDCLSGDVNK
                     DFSQLWKWISEFVPLPDDLPVGEPLTAESKMANA"
     gene            complement(3308..3523)
                     /locus_tag="VG754_10545"
     CDS             complement(3308..3523)
                     /locus_tag="VG754_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19699.1"
                     /translation="MSMVTDKRRIRRTKLPDLNRQVAQAKAVLRQLRDTLEDLDDRRD
                     LARARKKNAGKAGTDWEVVKKEMGLEF"
     gene            complement(3520..3756)
                     /locus_tag="VG754_10550"
     CDS             complement(3520..3756)
                     /locus_tag="VG754_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF19700.1"
                     /translation="MEIKDGAKAALNDLPPDLRRELGYRLHLLQKDFDGDVKKLKGSK
                     NEYRLRVGNYRVLFELVGKRIVVYTLGPRKDIYR"
     gene            3876..5219
                     /locus_tag="VG754_10555"
     CDS             3876..5219
                     /locus_tag="VG754_10555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF19701.1"
                     /translation="MNIVQITPGAGAMFCGNCFRDNALVAALRRQGHSVLMVPLYLPL
                     TLDEEDQTQGVPIFFNGVNVYLEQKSEWYRTAPRWLHRLFASPSLLKFASGRAAKTRA
                     ADLGDITLSMIRGEDGNQIRELDELIAFLKTQPKPDIICLSNALLAGLARKLKSELGV
                     PIACVLQGEDTFLDVLPDSHRALTWKTLAERCADIDLFIPPTQYFARLMRERLNLPEH
                     RVRVIYNGINLDGFELSTLNHQSSTTPTLGYFARMCREKGLDTLVEAFIILKERDTVK
                     NLKLKIGGGCGPSDLPVVERLRDRLRAKVFLQDVEFHPNVDRAAKQAFLRSLSVFSVP
                     ALYGEAFGLYVIEALASGVPVVQPRHAAFPELVEISGGGVLCEPGDAKALANALEPLL
                     LDSNNARALGAAGRKAVEEKFNAQAMANETIRAFEETVETAKPFKQKKMSSTKPG"
     gene            5226..5387
                     /locus_tag="VG754_10560"
     CDS             5226..5387
                     /locus_tag="VG754_10560"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HWF19702.1"
                     /translation="MVRAGNGKRVLVFDVCGNDYGLIVAMHFDRHRVYTLRFMTHAEY
                     SKDRWKSEL"
     gene            5384..5806
                     /locus_tag="VG754_10565"
     CDS             5384..5806
                     /locus_tag="VG754_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWF19703.1"
                     /translation="MKTKTKCQFQNLPKDYRGLIALLPPRPIHDRADYANVTEVTDAM
                     ILWNDEFGPDQADYFEALCSFIEKYDAEHVRWPAVSGRDMLRHLMEEPKMSAADLSRI
                     LGGSRNLGAMILRGNRNLTLNHIRTLAKYFGVPAGLFV"
     gene            5803..6246
                     /locus_tag="VG754_10570"
     CDS             5803..6246
                     /locus_tag="VG754_10570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWF19704.1"
                     /translation="MNYEFKAVRRVEFSETDMAGIVHYSNFFKYMETAEHGFFRALGH
                     SVVMNQFDPPLGWPRVRAECEYRQPLRFEDEVEIHMLISEKKSKSLSYIFKFRKLNGP
                     APVEVARGSLTVVCVTKQPDGKFVTAAIPKAFADKIEVAPAELLA"
     gene            complement(6266..6592)
                     /locus_tag="VG754_10575"
     CDS             complement(6266..6592)
                     /locus_tag="VG754_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19705.1"
                     /translation="MKTSLILMMMLAAGTLAAQPDRRTNEAATSAPTPHGVAGLLFQP
                     EKPNEIIRGRVTYSGVAVQIIKTKKPLQLINPLAPPEYGYGSDNLDRDIITGKPNGFK
                     LFSINF"
     gene            complement(6665..6874)
                     /locus_tag="VG754_10580"
     CDS             complement(6665..6874)
                     /locus_tag="VG754_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19706.1"
                     /translation="MAATLAEVSAHFGDPHRHAGLGLRKLSPGLWECRLDIRSRLVFM
                     KEKDRLIAYDLMNHDEIRAWLKNIK"
     gene            complement(6943..7224)
                     /locus_tag="VG754_10585"
     CDS             complement(6943..7224)
                     /locus_tag="VG754_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HWF19707.1"
                     /translation="MTEQVRYRIDRRLVAQAEKVCGELGISPSQAVSLFFAQLVKLGG
                     LPFRPSAFPALDEYGVTLDEAKAKEREVRRELELDEKAGKLGEFKGKLR"
     gene            complement(7275..7688)
                     /locus_tag="VG754_10590"
     CDS             complement(7275..7688)
                     /locus_tag="VG754_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19708.1"
                     /translation="MQRLINVKQLFVGLLAVLVLGGCTTNTVQSRKQKRYAAYQSFPS
                     DIKTAVDQGRITAGMNMDAVYIAWGNPDQVVTGGNQAGEAITWLYHGSYLGETSIWGD
                     RHVNYAYYSGGYVRAQVVFVNGVVKEWQTFPSPVY"
BASE COUNT         1824 a         2228 c         2008 g         1797 t
ORIGIN      
        1 cttaaacttt gaactaatcc gatgagcaca acacctccga ccgacgccct gcccaaatcc
       61 ccgcccttgg aaaagcaaac cactgtcggc aattatttcg tctccaatta tccgccgttc
      121 tcgttttgga aacaggaact cgtcaccgac ctctacgccg cgctggaacg cccgcacaag
      181 cccggcaatc cgctcggcat ttatctgcac atcccgtttt gccgcaagcg ctgtcatttt
      241 tgctatttcc gcgtttacac cgacaaggac tcggcagcca tcaagtctta catcgatgcc
      301 tctttgcagg agttgaagat ttatgccggc aagccggcca ttcgcgggcg caagccgaat
      361 ttcatttatt tcggcggcgg cacgccttct tatctttccg ttgaccagct caagcacctg
      421 ttcagtggca tgaaagagat gctgccgtgg gacgaagcag aggaggtcgc gttcgaagcg
      481 gagccgggca cactcaccga tcaaaagctg aaagcaatcc gtgatttggg tgtcacgcgg
      541 ttgagcttgg gcgtggaaaa ttttgacgat cacattttgg aaatcaacgg ccgcgcgcat
      601 cacagcaagg aaatcgcccg cgcctatggt tacgcgcgcg aactaaattt tccgcagatc
      661 aatattgacc tcatcgcggg gatggtcgag gaaaccgagg ccaactggcg cgaatgcgtg
      721 cgaaagacca tcgagatgtc gcccgacagc gtgacggttt acgagatgga aattccctac
      781 aacaccacca tctacaaaca gatgaaggcg gagggcaagc tcgtcgcgcc ggtggcggat
      841 tgggaaacca agcgcgcatg ggtcaattat gctttcaacg aattggagaa agcaggctac
      901 acgatttcca gcgcttacac ggccatcaaa aatccgcaac gcaccaagtt catttatcgc
      961 gaccgtcttt gggcgggggc ggatttgctt tcgctcggcg tcgcttcatt cgggcacatc
     1021 aacggcacgc attatcaaaa tcatcacgat ttcgatccgt atcttacgcg tgtgcagcag
     1081 ggcgagcttc ccgtttaccg cgcgctcacg ccgaatgatg acgagcgact catccgcgaa
     1141 ttcgtattgc aactcaagct cggccacgtg agccggaatt atttcaaaca aaaattcggc
     1201 gtcgaacccg ccgaacgttt tgccgcgccg ctgcaaacct tgaaagactg gggattcctt
     1261 acgattgacg acgacaccgt ccgcctgaat cgcgaagggt tgctgcaagt ggaccggctt
     1321 ttacaggaat tttttctgcc gcaacacaag aacgtgagat atgcctgaaa gagcctcaac
     1381 cattcccgct tcaaccacca caaatttttc gctcgcgcat ccgctcgacg aattttacgc
     1441 ggaagaagga caacctgtgc cgccgctgaa ccaaattgac ggcgaagcgg tgcccgaacc
     1501 gtatcgcacc ctgctcgttc accagaacga catgacgcca acattggaag catttcatcg
     1561 gtcgtccgtt cacctggatg tcttgggccg gcgtcgcaag ggcgacgctt atttccgcga
     1621 ggtcatcctt cgcctcgatt cgaatgatca accagttgaa tttggcgcga tcaaaatcaa
     1681 tctcgcgctt ttcacgcccg aggcgcgtga atacatcctc cacgaaaaat tgccgctcgg
     1741 ccacattttg caccagtata aaatcgccca tgcaagccgt ccgcgcgctt atctgcggct
     1801 ggcatcggac aaattgatca gcaaggtact caatctgaac ggcgcgcaag tgctctatgg
     1861 ccgtcgcaac accctttacg atccgcagca acggccgctt gcggaaatcg tcgagatttt
     1921 gccggtggcc gcaaccaggc attgaatcaa tgcgagaaaa cgacaatatc aatctcttcc
     1981 tgacttcctg atttcctaat taaacaatga actctaattc caagtccgac atcctcatca
     2041 tcggtgccgg gccagccggt tcaactgcgg ccgcgttgct cgcggaaaaa ggccatcgcg
     2101 tgctcgtgct cgaacgcgaa aagtttcccc gataccacat cggcgaatct ttgctgcctt
     2161 tcacttatca accgctcgaa cggcttggct tgattgaccg gatgcgaaaa tcggctttcg
     2221 tcaaaaagta cagcgtccag tttgtttcgc cttcaggccg cgcgagtttg ccattttatt
     2281 ttttcaaccg ctatgaccgc gaaagtgttg cgcaaacctg gcaggtgttg cgctcggagt
     2341 tcgaccagat gctgctcgac aacgcccgcg caaagggcgc cgaggtgaaa gaggaagtca
     2401 cagtgaagga attgatccgc gaacacggac gcgtcgttgg cgcgcgctcg caattgaaag
     2461 atggcacccc gacagatttt cgcgcgacca tcacgctcga ttgcaccggc aaagaagcgt
     2521 tcaccgccgt tcgcaatggc tggcggatga aagacccgta tctcaacaaa atcgccgttt
     2581 ggacttatta caaaggctcg aaacgcgacg aaggcgttga cgaaggcgcg accacagttg
     2641 catatgttcc ggaaaagggc tggttctggt acatcccgca acataacgat atgatcagcg
     2701 tcggcgtcgt tgcggaagga aaatatctga cgcgcgacgg cgtgaaatcc cccgaggaca
     2761 ttttcaagcg cgagattgag cagaacctat ggatcaagga acacctcgct tgcggcgaac
     2821 aaaccgggtc ttattttctc accagcgaat attcccatca ttccaaacat tgcgcgacgg
     2881 atggcttgct gctcgtgggc gatgcttttg cgtttctcga cccggtgttt tcctccggag
     2941 tgatgcttgc gcttaaaagc ggcgtgcttg ccgccgatgc cgtgcatcaa gcgttcgtgg
     3001 aaaaagattt ttcgccggaa cgcttctcgg attacgcctc gttcctgcgc cagggagtcg
     3061 aaaacatgcg caaactcgtt tatgcctttt atgatccgaa tttttcgttc ggcaaggtca
     3121 tcaaaaaaca tccggatgcc gccggactca tcaccgattg cctttccggc gacgtgaaca
     3181 aggatttttc gcagctctgg aaatggattt cggaattcgt gccattgccg gatgatttgc
     3241 ccgtgggaga gccgttgact gctgaatcaa aaatggcaaa cgcgtgagag tggacaatgc
     3301 ggttcggtca aaactccaaa cccatctctt tcttgaccac ctcccaatca gttcccgctt
     3361 tgccagcatt tttcttcctg gctctggcga gatcgcgccg gtcgtcaagg tcttctaaag
     3421 tgtcccggag ctggcgcaag accgccttgg cctgcgcaac ctggcgattc aaatcgggca
     3481 atttggttcg tcgaatcctg cgcttgtctg tgaccatgct catctgtaaa tgtcctttct
     3541 tgggccaagt gtataaacca caatgcgttt gccgactaat tcaaatagta ccctgtaatt
     3601 gcccacgcgc aaccggtatt catttttgct gcctttgagc tttttcacat cgccatcaaa
     3661 atctttttgc aataggtgaa gacgataacc caactcgcgg cggagatcag gcggcaaatc
     3721 attcaaagcc gccttggctc cgtccttgat ttccatttca tattccaccg cgttgaaaga
     3781 aaaccatgaa cacacgcctc atgtccacgc ccaaaataaa aaagctcgca tgactttcaa
     3841 ccatacctct ggcacattct cgtgagttcg cgcacatgaa catcgtccag atcacacccg
     3901 gcgcgggagc catgttttgc ggaaattgct tccgcgacaa cgcgctcgtc gccgcgttgc
     3961 gacggcaggg ccattctgtt ttgatggtgc cgctttacct cccgctcaca ctggatgaag
     4021 aggaccagac gcagggcgtc ccgatttttt tcaacggcgt caatgtctat ctcgagcaaa
     4081 aatccgaatg gtatcgcacc gccccgagat ggttgcatcg cttgttcgct tccccgagcc
     4141 tgttgaagtt tgcctccggt cgcgccgcca aaacccgcgc agccgatctc ggcgacatca
     4201 ccctctccat gatccgcggc gaagatggca accagatccg tgaactggat gaactcatcg
     4261 cgttcctcaa gacgcaaccc aaaccggaca tcatctgcct gtccaacgcg ctgcttgccg
     4321 gccttgcgcg caagttaaaa tcggaattgg gcgtgcccat cgcctgcgtg ttgcagggcg
     4381 aggacacttt tcttgacgtg ctgcctgatt cacatcgcgc gctcacttgg aaaacgctgg
     4441 cggaacgttg cgcggacatc gatctgttca tcccgcccac gcaatacttt gcgcggctca
     4501 tgcgcgaacg tctcaatctg cccgaacacc gtgtccgtgt catttacaac ggaatcaatt
     4561 tggatggttt cgaactctca actctcaacc atcaatcgtc cacgactccc acgcttggct
     4621 atttcgcgcg catgtgccgc gagaaaggac tcgatacgtt ggtcgaggcg ttcatcattt
     4681 tgaaagagcg cgacaccgtc aaaaatctaa agctcaagat tggcggcggg tgcgggccgt
     4741 ccgatttgcc ggttgtggaa cgattgcgcg accggctcag ggccaaggtg tttttgcagg
     4801 acgtggaatt tcatcccaac gtggaccgcg cggccaagca ggcgttcctg cgctcgctct
     4861 cggttttttc cgtgcccgcg ctgtatggcg aagcatttgg attgtatgtc atcgaggccc
     4921 tggcaagcgg tgtgcccgtc gtgcaaccgc gtcatgcggc attccctgaa ctggtcgaaa
     4981 tatccggcgg cggtgttctt tgcgagccgg gcgatgcaaa agcgctcgcc aacgcccttg
     5041 aaccgctgct gcttgattcg aacaacgccc gcgcactcgg cgcagccggg cgcaaagcgg
     5101 ttgaagaaaa gttcaatgcg caagcaatgg ccaatgaaac catccgcgcg tttgaagaaa
     5161 cggtcgaaac cgcaaaacct ttcaaacaga aaaaaatgag ttcgaccaag ccgggttgaa
     5221 atgacatggt gcgcgctggc aacggcaagc gagtgttggt ttttgatgtt tgcggcaatg
     5281 attacggact tattgtggcg atgcactttg accggcatcg ggtttacacg cttcgcttta
     5341 tgacgcacgc cgaatacagc aaagaccgct ggaaaagcga actatgaaga caaaaaccaa
     5401 atgccagttt cagaatttgc ccaaggatta ccgggggttg attgcgttgc tcccgccgcg
     5461 tccgatccat gacagggctg actacgccaa cgtgactgaa gtcactgatg ccatgatcct
     5521 ctggaatgat gaattcggtc cggatcaagc cgactatttt gaagcccttt gctcgttcat
     5581 cgaaaaatac gacgccgaac atgtccgttg gccggcagtg agcggacggg acatgctgcg
     5641 gcacctgatg gaagagccaa aaatgtctgc ggcagaccta tcacgcatcc ttggcggaag
     5701 ccggaatttg ggcgcgatga tcctgcgcgg caaccgcaat ctcaccctca accacatccg
     5761 gacactcgcc aaatatttcg gtgtcccggc gggattgttt gtatgaacta cgaattcaaa
     5821 gccgtccggc gcgtcgagtt ttctgagact gacatggccg gcattgtcca ttactccaat
     5881 tttttcaagt acatggagac ggctgagcac ggtttttttc gcgcgctcgg ccattcggtc
     5941 gtgatgaacc agtttgatcc gccgctcggc tggcctcgtg ttcgcgccga atgcgaatac
     6001 cgccaaccgc ttcgtttcga ggatgaagtc gagatccaca tgctgatcag cgaaaaaaaa
     6061 tccaaatcgc tcagctatat tttcaaattc cggaaattga atggtcccgc tccggttgaa
     6121 gtcgcgcgtg gttcgttgac ggtggtatgc gtgacgaagc agcccgatgg aaagttcgtc
     6181 accgcagcca ttcccaaggc gttcgcggac aaaatcgaag tcgcgcctgc tgaattgctg
     6241 gcgtgaatgg aaaatccggt gctgctcaaa agttgatcga gaataatttg aagccgtttg
     6301 gtttgccggt gatgatgtcg cggtccaaat tgtcgctgcc gtagccgtat tccggcggcg
     6361 caagcggatt aatgagttgc agcggctttt tcgtcttgat gatctgcacg gccacaccgc
     6421 tgtaagtcac gcgtcccctt atgatttcat tcggcttttc cggttgaaag aggagtcctg
     6481 ccacgccatg cggagtcggt gcagaagtgg ccgcctcgtt cgtcctccga tcgggttgcg
     6541 ccgccaacgt gccggcggca agcatcatca ttagaatcag ggaggttttc atacgacgtg
     6601 ttttggcgct aaataaattt cgaaccaaac cgtcggaaag tcaacccggt gaaagccgct
     6661 ccagctattt gatgttttta agccacgccc ggatttcatc gtggttcatc aggtcgtacg
     6721 cgataaggcg gtccttttct ttcatgaata caagccttga gcgaatgtcc aggcggcact
     6781 cccataatcc gggcgaaagt tttctcaatc caaggccggc atggcgatga ggatcgccga
     6841 agtgggctga tacttcggca agcgtcgctg ccaatcgttc acgcgaatct gaatcgagct
     6901 tttgaatcga gcgggaaaag cgagcgccaa aaaaaatccg gatcaccgca atttcccctt
     6961 gaactcaccc aattttcccg ctttctcgtc caactccagt tcccgtctga cttcccgttc
     7021 cttagccttt gcctcatcca aggtgactcc atactcatcc agcgcgggaa atgcggaggg
     7081 ccggaaagga agtccgccga gtttgacaag ctgggcaaaa aaaagagata cggcctgcga
     7141 cggagagatc ccaagttcac cacaaacttt ttcggcctgg gcaaccagcc gccggtcaat
     7201 tcgataacgc acttgctcag tcatgcctca aatatgctca gcaatgggcc ttgcgtcaag
     7261 gcaagacagt cgcgtcaata caccgggctg ggaaacgtct gccattcctt gaccactccg
     7321 ttcacaaata ccacctgcgc gcgaacatac ccgccggaat agtaggcgta gttgacatgc
     7381 cgatcacccc aaatgctcgt ttcccccaaa tagctgccgt gataaagcca tgtgatcgct
     7441 tcaccggctt gattgcctcc cgtgacgacc tggtcgggat ttccccaggc gatatacacc
     7501 gcgtccatat tcatcccggc cgtgatgcgg ccttgatcaa cagccgtctt tatgtcggaa
     7561 ggaaatgatt gataagcggc atagcgcttc tgtttgcggg attggaccgt gttagtggtg
     7621 caaccgccca acaccagcac ggccagcaat ccgacaaaca actgcttcac attgataaga
     7681 cgttgcatgg catccattat tcacaactcg tccgcgattt aaagccacaa agcatttgcc
     7741 gtaaaggcgc tccaactcta taatcaccca ttatatgaaa tggctcgtat tcgcgctgat
     7801 gactgttgtt tcgtggggac tctacggcat tttcctgcac agcggccagg tcggaat
//
LOCUS       DASZMM010000438         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_176080, whole genome shotgun sequence.
ACCESSION   DASZMM010000438 DASZMM010000000
VERSION     DASZMM010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176080"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1698)
                     /gene="tatC"
                     /locus_tag="VG754_10595"
     CDS             complement(229..1698)
                     /gene="tatC"
                     /locus_tag="VG754_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWF19709.1"
                     /translation="MVIEPEEERPPFEEDEGGPVKSFLEHLEDLRWVLIKSVSTIAVA
                     MLVCLIGGNYVVGILKRPLEKAQARYSSDEKIAVFRYGTNRLGTFSLDLDQWDSLVAP
                     PDRPNPWRIATNEIVNLRSLAAKLQDPDKAGKTAELSRFISDHLSPETRVLVTNFNAG
                     MKRQGLLHLFFPAPGEEERMSKVTADLTRSLTDDLGRIIESGPVYTEKRFADITLSTD
                     TDRLRTANPQGTDLFRLNRKLLLHAYPGELTSNGQRFVDVQLEPKSDGTNLVLGMHVD
                     KNADAEALAKQMQVDLNTLSPAGGFFVAFQVAMYAGIALSSPLLFYFIAQFVFPALKW
                     RERRYVFRGMFFFVPLFVIGASFCYFALLPVALAASQVYSHWLGFSANIWQAEEYISF
                     VSKFILGMGLGFEMPVVILVLVKIGIVDYAMLAKGRRYMILINLILGALLTTPEVLTQ
                     VLMAVPLQLLYEISVWIAWYWERKEKKRMAAEEAAERNM"
     gene            complement(1716..2009)
                     /locus_tag="VG754_10600"
     CDS             complement(1716..2009)
                     /locus_tag="VG754_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWF19710.1"
                     /translation="MNTMLAGFFGGWEIVLILAMALLLFGAKKLPELAKGLGQGIKEF
                     KKATREVTDEMENAMHDNNPPPPPQKTLPPGNTIVTSSEPAQAVPQNPPGQKA"
     gene            complement(2130..2804)
                     /gene="pal"
                     /locus_tag="VG754_10605"
     CDS             complement(2130..2804)
                     /gene="pal"
                     /locus_tag="VG754_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWF19711.1"
                     /translation="MLPGLRAAKATPNPVGQGDVKVTFPTKTKRMKLKNLLNLMVLGS
                     ALTITAVGCRTKPLNPMLLPNRNLPPRDANALDQPKPFDTGSSSVTTTGIPPTTNDYS
                     TYIPDVSALKAQTVYFDFDKSAIKSSEQSKLEDVANYMKSHGDAAVRIEGNCDERGTE
                     EYNRSLGERRALSAREYLVRLGIDPNKIPTISYGNDKPAEPGHNESAWSKNRRDDFVV
                     LNPPKP"
     gene            complement(2749..>3691)
                     /locus_tag="VG754_10610"
     CDS             complement(2749..>3691)
                     /locus_tag="VG754_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19712.1"
                     /translation="LKGNNNGSVQGELSFGKNVIFSKIYSGGSLRAQAHRLADDVVLK
                     LTGVNGIAETKIAFKVGSGPSSEIYVADFDGHDARAVTADGTTVAAPCWVPGKLALFY
                     TSYKLGNPDVFYQNIATGHRQNFSHYPGLNTSAAVSPDGNRIALVLSKSGNPDIFVRD
                     IETDNLKQLTTTREASSPCWSPDGKWICFAARVNGRRSLYKVPAEGGPMERISTSGIS
                     NPSEPDWSPDGKWIAFTAQMGDFSICVVPAQGGSATPLVSGEDPSWSPNSRTLIFVRR
                     GRGGDRMLSVLDVPTKQVKDAARVAGSESNSQPSWAR"
BASE COUNT          864 a         1056 c          948 g          823 t
ORIGIN      
        1 cgtgccgccg tgaccgccaa tgactccgca gacgcgcagt gatgcctcac acagctttca
       61 atcggagggc aggtttcatt catgcccctc cttctaactg ataaccccac tctttgcccg
      121 ctcaaaaact gagatgcgcc cgaagtcggg gaggaggttg catcgttaca agggctgccg
      181 attggaatgc cttgcccatg caatgattta accatggaac caattcaact acatattccg
      241 ttcagcagcc tcttccgcgg ccatgcgctt cttctctttc cgttcccaat accacgcgat
      301 ccagacgctg atttcgtaaa gcaattgcaa tggaacagcc atcaagactt gggtgaggac
      361 ttcgggcgtg gtcaaaagcg cgcccaagat caaattgatg aggatcatgt atcgccggcc
      421 ctttgccagc atcgcataat caacgatgcc aatcttgacc aggaccaaaa tcaccaccgg
      481 catttcgaag ccgagcccca tgcccaggat gaatttggac acaaaactga tatattcctc
      541 cgcctgccaa atgttcgcgg agaacccaag ccagtgtgaa tacacttgcg acgccgccaa
      601 tgccacgggc agaagcgcga aataacaaaa gctcgccccg atcacaaaca acggcacgaa
      661 aaagaacatc ccgcgaaaca catagcgccg ttcccgccat ttcaacgccg ggaaaacaaa
      721 ttgcgcgatg aagtaaaaaa gcagcggcga ggacagggcg atgcccgcat acatcgccac
      781 ttgaaacgcc acaaaaaatc ctccggccgg gctgagcgtg ttcaaatcca cctgcatctg
      841 cttcgccaac gcttcggcat ccgcattttt atccacgtgc atcccaagca ccagatttgt
      901 cccgtcagac ttgggctcaa gctgcacatc cacgaaccgc tgcccattgc tggtcaactc
      961 gcctggatac gcgtgcaaca ataacttccg gttcaacctg aatagatccg ttccttgcgg
     1021 atttgccgtc cgcaaccggt cggtgtcggt cgaaagagtg atgtcagcga aacgtttttc
     1081 agtgtaaacc ggaccgctct cgatgatgcg gcccaggtca tcggtcaatg accgcgtcaa
     1141 gtccgcagtc actttgctca ttctttcctc ttcgcccggc gcgggaaaaa acagatgcaa
     1201 caatccctgt cgcttcatgc ccgcgttgaa gttggtgacg agaacgcgag tttccggcga
     1261 aagatggtcg ctgatgaaac gggacaattc agcggttttg ccggctttgt cagggtcttg
     1321 gagttttgca gccaatgacc tgagattgac gatctcattg gtagcgatcc gccacggatt
     1381 cggacgatcc ggcggcgcga cgagagaatc ccattggtcc aaatcgaggg aaaaagtgcc
     1441 aagccggttc gtgccgtatc tgaaaacggc aatcttctca tcgctggaat agcgcgcctg
     1501 cgctttttcg agcgggcgct tgaggatgcc gacgacgtaa ttacccccga taaggcagac
     1561 caacatcgcg acggcaatcg tcgaaacgct tttgatcaag acccagcgaa ggtcttcaag
     1621 atgctcaagg aaggatttta caggcccacc ctcgtcctct tcaaaaggag gacgctcctc
     1681 ttcgggctca atcaccatgg cagatcattg cctgatcagg ccttctggcc gggaggattt
     1741 tgcggaaccg cttgcgcagg ttcgctgctg gtgacgatgg tattgccggg aggaagggtc
     1801 ttttggggcg gaggcggagg gttgttgtca tgcatcgcat tctccatctc atcagtcact
     1861 tcgcgcgtgg cctttttgaa ttccttgatg ccctggccca atcccttggc caattcaggc
     1921 agcttcttgg cgccaaacaa aagcaacgcc atggcgagaa tcaaaacgat ttcccaccca
     1981 ccaaaaaatc ccgctaacat tgtattcata aatctttcct aagttgtaat gacgataagc
     2041 ccgcgcaccc cattagtaaa gcaccaaaat aggccaaaaa ggggcacaaa aaacgcaatt
     2101 ttggggaaca ctcagctatt ccgcgtctct tacggcttcg gcggatttaa aaccacgaaa
     2161 tcatcccgac ggttcttcga ccaagcggat tcgttatgac cgggttccgc aggcttgtcg
     2221 ttgccgtaac tgatggtcgg aattttgttg ggatcaatgc ccaaacggac caaatattcg
     2281 cgagcagaga gagcccggcg ttcaccaaga gaacgattat attcctcagt gccgcgttcg
     2341 tcgcaattgc cttcgatacg tacagcagca tcgccatggc tcttcatgta attggcgaca
     2401 tcttcaagtt tcgattgttc actgcttttg atggccgatt tgtcgaaatc aaaataaacg
     2461 gtctgggcct tcaaagccga cacgtctggt atgtaggttg aataatcatt tgttgtcggg
     2521 ggaatgccag ttgtggttac actggacgac cctgtatcaa agggtttggg ctgatcgagt
     2581 gcatttgcat cgcgcggcgg caagttgcga ttcggcagca acatgggatt cagcggcttg
     2641 gtgcggcaac cgacggcggt gattgtcagg gctgacccta acaccattaa attcaacaga
     2701 ttcttaagtt tcatgcgttt ggttttagtc gggaaggtca cttttacatc accttgccca
     2761 actgggttgg gagttgcttt cgctgcccgc aacccgggca gcatccttga cttgtttcgt
     2821 tggcacgtca agcacagaga gcatccggtc tccccccctg ccgcgcctca caaaaatcaa
     2881 tgtccgcgag ttcggcgacc acgacgggtc ttcgccgctg accaaaggcg tggccgaacc
     2941 gccctgcgcc gggaccacgc aaatgctgaa atctcccatc tgcgccgtga acgctatcca
     3001 cttcccgtcc ggcgaccagt ccggctcgga cggattggag atgccgctgg tcgagatgcg
     3061 ttccatcggg ccgccttcag cgggaacttt gtaaagtgaa cgccgaccgt tcacgcgcgc
     3121 cgcaaaacaa atccattttc catccggcga ccagcagggc gatgacgcct cgcgcgtggt
     3181 ggtgagttgt ttcaaattat ccgtctcaat gtcacgcacg aaaatgtccg gattgccgga
     3241 cttgctaagc acgagcgcaa tcctgtttcc atccggcgaa actgcggcgc tggtgttcaa
     3301 gccgggatag tgcgaaaaat tttgccgatg gcccgtcgcg atgttttgat aaaaaacatc
     3361 cgggttgcca agcttgtacg aagtgtaaaa cagcgcgagc ttgcccggga cccagcacgg
     3421 cgcggcgacg gtcgtgccat ccgctgtgac cgctcgggcg tcgtgaccgt caaaatcggc
     3481 cacataaatc tcgctggacg gcccgctgcc gactttgaaa gcgatcttcg tctccgcaat
     3541 gccattgaca ccggtgagct tcaacaccac gtcatcggcc agccgatgcg cctgcgcgcg
     3601 aaggctgccg ccgctgtaaa ttttcgagaa gatgacattc ttgccgaaac tcaattcgcc
     3661 ctggacgctg ccgttgttgt ttcctttaag t
//
LOCUS       DASZMM010000439         5611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2935937, whole genome shotgun sequence.
ACCESSION   DASZMM010000439 DASZMM010000000
VERSION     DASZMM010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2935937"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VG754_10615"
     CDS             complement(<1..472)
                     /locus_tag="VG754_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003784171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19713.1"
                     /translation="MAINQSTWQSFDEIKKAAEAGDPASQCYIGICYQTGQGVPLNYE
                     EAVKWLRMAAEQNDSSAQCYLGVCYQAGLGVPQEFSEAAKWFREAADQDDPAAQFNLG
                     VLYETGQGLMQNYAEAVKWYHSAAEQGEPQAQFNLGVFYETGQVVPQDYAEAVKW"
     gene            1045..2466
                     /locus_tag="VG754_10620"
     CDS             1045..2466
                     /locus_tag="VG754_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19714.1"
                     /translation="MNAHQSYRNLPPLAGICSMAEAMQPGLSVEECVARLKRYHYAFK
                     RLHQIFTARLTAEPIYELKMAFSLHAHLCAEHVSSLRKRVGEMREPPLGLEAVPNASL
                     EIFFDEILAAPTTTELVLGIYEKALPALKAALEQHLNDTNPLADHPSARLCKFALLEV
                     GEMLDFGAKAITSLVDRASRAQTMPWLKLFDDCLAAAGSLDGAENISAAPQRMHSAKP
                     FQYDGVPKRDERFADPYNMGVNAEVFLYDEKFPPEPKTLMMFYKRLREIDVPEMMASI
                     IVETREKPWDYYRDMTRQLWDEARHAMMGEVGFANMGIDWPKKVMINYTWSLALNTQL
                     KPIERHAVLYFIEQGLMPKTGKRFEWEVGLASHNPLSALFQDYDWADEVLHARIGRDW
                     YLKEFGNAQQAVRYGDECWSRVLMSWENWRQQGLTQHRNWWPEVYLDACKHWRSEPDP
                     KVLAYSTSYQTVRADLKNLVGSA"
     gene            2765..3724
                     /locus_tag="VG754_10625"
     CDS             2765..3724
                     /locus_tag="VG754_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWF19715.1"
                     /translation="MLSAAELAVTEVPANSSSQQIQEALNKLPNEGGEVRLSAGTYNI
                     SQPIVLRRDNQVLRGSGTATVLQLADNADCPVVILGAPLLPNRIPSHLRLSGVFIDGN
                     RFHQKSEMWKSAPDGALLNNNGIIAWGVKDAFIEDVTCCRCRSGGLVTAHGVRLLTVH
                     DFKAFDNQYDGLACYQTENSQFTKLYLHDNLCAGISLDLDFNHNVLRDAQLTNNDLGV
                     FMRDSHDNVFQALKIQHSRTHGVFMAQAGDQTARGWRFVPGTQCTGNTFNGLVITDCG
                     GKAVLVNDGSCTNNTVISAKFKGNAHGDLAEAAPNLMTMLSLP"
     gene            complement(3835..>5611)
                     /locus_tag="VG754_10630"
     CDS             complement(3835..>5611)
                     /locus_tag="VG754_10630"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWF19716.1"
                     /translation="QQSGTQASVYYCVNSLQNGSDNKGHAENNPNDAPAGEITQMVRE
                     AYWAWHVSCWNPWMFGTEHEGFVDSPVWYSEAMYQASAALQQHLCNGPNANTPPIPMD
                     RNHIIGHDEWQNPAWTNWMATNWPQIDTTCNNHTDPGQYWNWPHFMSLVCMPKILSQP
                     VDQTVTQGQPAMFSFSVTNVGTNNPTTYQWMLDGAIIPGATNATLNLANVQPGDAGSY
                     SVVLSNAYSSVTSSVAALDVTAAITDLAVQPRPSSAIITWNTDSPSDSEVDYGLTTNY
                     EAGYTYDGNFVTNHVALLTGLIPNQTYYFQVISFDGDGNEADATSSFSTANSIIVPSL
                     RASYLGVWVNDSSAADRYSPIYKYASVGPAAPSESATFRPNILTPAQYDVYLWYSEGG
                     NRSQLAPVTATFQNGYLSTVINETLKGGKWQLLTSNQSFAAGTNGFVRLGNYTGESNK
                     IVISDAVEWVYTAGQDIPVNGNVPAWWANFYFGTNSVDGSAPAANGYSIMNDYILGIA
                     PDDPDTTLDFSLAPYNNGFQATFYPWAGGRIYQLQSTTNLAHPVWQTLTNLPVTTDDF
                     GDGLITSTNAPAVPTFYRLSVQLMP"
BASE COUNT         1258 a         1474 c         1554 g         1325 t
ORIGIN      
        1 accatttgac ggcttcggcg taatcctgcg gcacgacctg gccggtttcg taaaagacac
       61 caagattaaa ttgcgcctgc ggttcgccct gttcggcggc ggagtgatac catttgacgg
      121 cttcggcgta attttgcatc aggccctggc cggtttcata gagaacgccg aggttgaatt
      181 gcgcggcagg atcgtcctgg tccgcggctt cgcggaacca cttggcggct tcgctgaatt
      241 cctgcggcac gccaagcccg gcttggtaac agacgccaag ataacattgg gcggaagagt
      301 cgttttgttc agccgccatc cgcagccatt tgaccgcctc ttcataattc aacggcacgc
      361 cctggcccgt ctggtaacag atgccgatgt agcattgcga cgcaggatcg ccggcctcgg
      421 cggctttttt gatttcgtcg aatgattgcc aggttgattg gttgatggcc atggttttgc
      481 cgggttaagt gtttaaaact tcggcttttt agcgcactta gttatcataa tgagtcccaa
      541 ggcgaattca agcgtccatt ccattttctg gtgcgaccag tctcatccta ttagaccgaa
      601 ataaaaccgc ataggtaacg cgttatttaa taaaactgtt ttttccatgg cgatgatggc
      661 tatacccctc cccccctcga aaaaaagcga aaaaatgcga aaaaatgcga gaaaccccga
      721 gaaatcaagg gttccgtgga acatctgtcc agttccggac tgcgcacaat ttgcgcgcca
      781 gattttccag ttattttttc atctttcatc atcgcggagg attttaaatg acgacctcgg
      841 acaaagactg tccgaggtgc gcgaaaaaat atgtggcgtc aggcgtccct cctgacgcca
      901 catcaggaac aaaattcttg aggaaacatc gtcgtttcct gatttcctgc tttcctaatt
      961 ctatttccca cgggatggct ctgaaattta ttgtcgaaat tgcgcgacga gatgttaaag
     1021 ttcctatcaa agccctgagt aatcatgaat gcccatcaat cgtataggaa tctgccgccg
     1081 ctggcgggga tttgttcgat ggccgaggcg atgcagcccg gcctgtccgt cgaagaatgc
     1141 gtcgcccgcc tcaaacgata tcattatgcc ttcaaacggc tgcatcagat tttcaccgcg
     1201 cgcctgaccg ccgagccgat ctacgaactg aagatggcgt tcagccttca tgcgcatctt
     1261 tgcgcggaac atgtgtcgtc gttgcgcaag cgcgtcggcg agatgcgcga accgccgctc
     1321 ggccttgaag ccgttcccaa cgcaagcctt gaaatctttt tcgacgaaat tttggccgcg
     1381 ccgacgacga ccgaactcgt tctcggcatt tatgaaaagg cattgccagc gctcaaggcc
     1441 gcgttggaac agcatttgaa cgacaccaat ccgctggcgg accatccgag cgcgcgtctc
     1501 tgcaaatttg cgttgcttga agtgggcgag atgctcgatt tcggcgcgaa ggccatcacc
     1561 agcctggtgg accgcgcctc acgcgcgcag acgatgcctt ggcttaaatt gttcgatgat
     1621 tgccttgccg cagccgggag cctggatggc gcggaaaaca tttccgccgc tccgcaacgg
     1681 atgcattccg cgaaaccgtt ccaatacgac ggtgtcccga aacgtgacga acgatttgcc
     1741 gatccgtaca acatgggagt caacgccgag gtttttctct acgacgaaaa atttccgccc
     1801 gagccgaaaa cgctgatgat gttttacaag cgtctgcgcg aaatcgacgt tccggaaatg
     1861 atggccagca tcatcgtcga gacgcgggaa aagccgtggg attattaccg cgacatgacg
     1921 cgccagcttt gggacgaggc gcggcacgcc atgatgggcg aagtcggttt cgcaaacatg
     1981 ggaatcgatt ggccaaaaaa agtgatgatc aattatacgt ggtcgctcgc gctgaacacg
     2041 cagctcaagc cgatcgagcg ccacgcggtg ctttatttca tagagcaagg attgatgcca
     2101 aagaccggca aacgcttcga atgggaggtc ggattggctt cgcacaatcc gctttcggcg
     2161 ttgttccagg actatgattg ggccgatgaa gtgttgcatg cccgcatagg ccgggactgg
     2221 tatttgaagg agttcggcaa cgcgcaacaa gccgtccgct atggtgatga atgctggtcg
     2281 cgcgttttga tgagctggga aaactggcgt caacagggcc tcacgcagca tcggaactgg
     2341 tggccggaag tctatctcga cgcctgcaaa cattggcgca gcgagcctga cccgaaagtg
     2401 ctggcttatt caacttccta tcaaaccgtc cgcgccgatc tgaaaaatct tgtcggctcg
     2461 gcatgaggtt tgctgaaaaa ttaggcacga ggcttgctcg aaataagcca tcgcaagttt
     2521 gaaaatgtag tgtgcgtagg acgaacactt actcttgttg taagcggaat ggcgagttag
     2581 tgagtcggtg tcagtcgcgc aaagaaagtt tcgatttttt tgaatctttt gatgccgcaa
     2641 tcgtctatta cagcaaacct gcttgtttta tatttgcatg cttaaacagt cgttttttaa
     2701 tgagtgcttt gccgtccccg ctttgcgggc actgctctca attctagcaa ttttttcccc
     2761 ggctttgctt tcagccgccg agcttgccgt cacagaagtc ccggcaaatt cgagcagcca
     2821 gcagattcaa gaagcgttga acaaacttcc gaatgaaggc ggcgaagtcc gtctttccgc
     2881 cggcacttac aatatcagcc agcccatcgt gcttcggcgg gacaatcaag tgttgcgcgg
     2941 cagcggcacg gcgaccgttc ttcaactcgc ggacaatgcc gattgcccgg tggtgatcct
     3001 tggcgcgccg ctcctcccca atcgcattcc ctcgcatctg cgtttgtcgg gcgttttcat
     3061 tgacggcaac cgtttccatc agaaatccga gatgtggaaa tccgccccgg atggcgcctt
     3121 gctcaacaac aacggcatca tcgcatgggg cgtgaaggat gcgttcatcg aagacgtcac
     3181 ctgctgccgc tgccgttccg gcgggctggt cacggcgcac ggtgtccggc tgctgaccgt
     3241 tcatgacttc aaagcgtttg acaatcaata cgacggtctt gcctgctacc agactgaaaa
     3301 cagccagttc accaaattgt atttgcacga caacctttgc gccggcattt cgctcgatct
     3361 tgatttcaat cacaacgttc tccgcgatgc gcaactcacg aataacgacc tcggcgtttt
     3421 catgcgcgat tcgcatgaca atgtgtttca ggccctcaaa atccagcaca gccgcactca
     3481 cggcgttttc atggcgcagg ccggagacca gactgcgaga ggctggcggt ttgttccggg
     3541 cacacaatgc accggcaata cttttaatgg attggtcatc acggattgcg gcggcaaggc
     3601 ggtccttgtg aatgacggct cctgcacgaa caacaccgtc atcagcgcga aattcaaggg
     3661 caacgcccac ggcgaccttg ccgaagccgc tcccaatttg atgacgatgt tgagtttgcc
     3721 ttgagttgat cgaggctgat aattaaccac caagacacca ggtcacaaag aaagtgactg
     3781 atcgtgtgtt ggtggttttt ttatggcgat gaaccaggcc agcaactttt ttcttcaagg
     3841 cataagctgc accgaaagcc ggtaaaaggt cggcacagcg ggagcgttgg tggaggtgat
     3901 caaaccgtcg ccgaaatcat cggtcgtcac gggcaaattt gtcagcgttt gccacaccgg
     3961 atgggcaaga ttcgtcgtgc tttgcaattg atagatgcgg ccgcccgccc atggatagaa
     4021 cgtcgcctga aaaccgttgt tgtagggagc gaggctgaag tcgagcgtgg tatcgggatc
     4081 atcaggcgcg atgccgagga tgtaatcatt catgatgcta taaccgttgg cggcgggcgc
     4141 ggagccatcc acggagtttg ttccgaaata gaaatttgcc caccatgccg gcacgttccc
     4201 attgaccggt atatcctgac ccgccgtata gacccattcg acagcatccg aaatcacgat
     4261 tttattggat tcgccggtgt aattgccgag gcgcacaaag ccgtttgttc ccgctgcgaa
     4321 gctttggttg ctcgtgagca attgccattt tccgcccttc aatgtttcgt tgatgacggt
     4381 cgaaagatag ccgttttgga aagtggccgt taccggcgcg agctgggagc ggttgccgcc
     4441 ttcggaatac caaagataaa cgtcatattg cgctggagtg aggatgttgg gccggaacgt
     4501 ggctgactct gacggcgcgg caggcccgac tgacgcgtat ttataaatag gactgtaacg
     4561 gtccgcagcc gaagagtcgt tgacccagac tccgagatag gaggcgcgca aacttgggac
     4621 gatgattgaa ttggcagtcg agaagctgct tgttgcatcc gcttcattgc catccccatc
     4681 gaatgagatg acctgaaaat aataagtttg attgggaatc aagccggtga gcaaggcaac
     4741 gtggttggtc acgaagtttc catcataggt gtagccggcc tcgtaattcg tcgtgagccc
     4801 gtaatcaact tcggaatcgg aaggcgaatc tgtgttccat gtgatgatgg cactgctggg
     4861 gcggggttgc acggcaagat cggtaatcgc tgcggtgaca tccagcgcgg caaccgaact
     4921 ggtcacactc gaatacgcgt tggagagcac gacggaatac gatcccgcgt cgcccggttg
     4981 cacgttcgcg agattaagag tcgcattcgt cgcgccgggg atgatggcgc cgtccaacat
     5041 ccattgataa gtcgtgggat tgttcgtgcc gacgttcgtg acgctgaacg agaacatggc
     5101 cggctgacct tgcgtcactg tttggtcaac gggttgcgaa agaatttttg gcatgcagac
     5161 caaagacatg aaatgcggcc agttccaata ttggcccggg tcggtgtgat tattgcaagt
     5221 cgtgtcgatc tgcggccaat tggttgccat ccaattggtc caggcgggat tctgccattc
     5281 gtcatggcct atgatgtgat tgcggtccat gggaatcggc ggcgtgttcg cgttggggcc
     5341 attgcagaga tgctgctgca acgcggccga agcctggtac atggcttcgg aataccatac
     5401 ggggctgtcc acaaaacctt cgtgctcggt gccgaacatc cagggattcc aacaactgac
     5461 gtgccacgcc caatacgcct cgcggaccat ttgggtgatt tcgccggccg gggcgtcatt
     5521 gggattgttc tccgcgtggc ctttgttatc agagccgttt tgaaggctat tgacgcaata
     5581 ataaacactg gcttgcgtgc ccgattgttg g
//
LOCUS       DASZMM010000440         6044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2937216, whole genome shotgun sequence.
ACCESSION   DASZMM010000440 DASZMM010000000
VERSION     DASZMM010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2937216"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VG754_10635"
     CDS             <1..693
                     /locus_tag="VG754_10635"
                     /inference="COORDINATES: protein motif:HMM:NF017266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HWF19717.1"
                     /translation="LGAAANVLTVLRATRESHCPLRILSFDHTLEPLQFAFENAITLG
                     YFGGYEYIVETFLREYQVTFMDANRQITWELFLGDFPSLIRTPHSALRTSSPHSILFD
                     AFSPAKNPAMWTQPLFADLFRLLDPARPSAWPTYSRSTMLRVSLLLAGFYVGVGHATG
                     EKDETTIAANTLELLTEPLDHKWLQRARNSTSAEPMWEPVYRQAPLSAESWDKLQQHP
                     QFTSLSKTGKKC"
     gene            687..1889
                     /locus_tag="VG754_10640"
     CDS             687..1889
                     /locus_tag="VG754_10640"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWF19718.1"
                     /translation="MLMQFLVRAISTRSLKLVQKIRTTPVLLFAAIALMQFSAFGDEP
                     QEPLPFTGVFTNGSDGYPVFRIPAIVKTHQGTLLAFAEARAARADGSQNKIVLKRSHD
                     KSVTWEKAQLIWDDSANSLNNPTVVVENQTGRIVLIFQRYPKGIYENDVVPGLDGERI
                     CRAFVTYSDDDGLTWAKPTDITASVKRPTATSIASGPGIGIQLARGKHAGRIIIPFNQ
                     GPIATGKVYAVFSDDRGATWKYGQTAPGESKGSANEVQMVELSDGSVLLNARNQHGPH
                     VRKTAVSHDGGETWSTLQDEPQLIEPTCQASLIRHSTIDNPIEEILLFSNPASTTART
                     NGTIRLSRDEGKTWPISRVICPDRFGYSCLVSLDDKNIGCLFEHGQKICFCRFSLDWL
                     MDIKETAK"
     gene            1886..2953
                     /locus_tag="VG754_10645"
     CDS             1886..2953
                     /locus_tag="VG754_10645"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HWF19719.1"
                     /translation="MMIPSITTLAGQLDNLLADNANILALAPMQDVTDLAFWKLMARY
                     GGADVYFTEYFRVHAVSHLEKWILDSITQNPTAKPVIAQMIGNDIPSLVRSAKELQQY
                     PIAAVDLNLGCPAPIVYRKCAGGGLLREPKRVDSILGALRDAVSIKFTVKTRIGFDSP
                     AVFDELLPIFAKHSLDLLTVHGRTVQEMYRTEVHYDYIARAVAAVPCPVLANGNIYSA
                     GKAREVIQLTGARGLMIGRGAIRNPWLFRQIRQLQRGEPLFIPTGFEVLEYVRDLYET
                     VRPPHIKESAHIQKMKKYMNYIGLGIEPSGKFLHEIRRVTTETDFFRICETYLSHGQP
                     MPLEPFSLGLKETDVMAGEHL"
     gene            complement(3092..4216)
                     /locus_tag="VG754_10650"
     CDS             complement(3092..4216)
                     /locus_tag="VG754_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWF19720.1"
                     /translation="MYKKLATNGAIGRRSFMKRLGMAGAVALPAGALLSSGNSSRAED
                     RNKNLTKSDAALLRFLAAAEILETDLWQQYNELALGNESFQLALNILDGDEATYVDLN
                     TRDELSHATFLNAYLMFKGAKPVSLEPFRRLPSSQATGSNKTAKRLTNLMNLTVDTSW
                     YFRYRSSMNPDFGAMFPQIVNLVNVPGIPDSDLPPPVDADGNLLPSGFEIQFIGNVAG
                     FHFATIEQGGSNLYQSMLPKASSGEVIQIIGAIGGTEIQHFQTWQDKAGNALPVTDPM
                     GNVLFPQLPLAPALPNPPDGTDNAGPNDTNQIFATPCEFINKDLPACSVIRPISTQQA
                     GAVATVNFFTAMGLFQGQDQSFFNFLNELAQEADLARRDG"
     gene            complement(4402..4830)
                     /locus_tag="VG754_10655"
     CDS             complement(4402..4830)
                     /locus_tag="VG754_10655"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWF19721.1"
                     /translation="MNLLQRIRAWREDKEQPLAHRHGELGERAAKKHLQRLGLKFLTA
                     NYCSERGEIDLVFRDEDCLVFVEVKTRSSEEWTRPAAHVNARKKRLLSQTALDYLKLL
                     KNPPVKIRFDIVEVLLKESEVREVRHLPNTFAMSKPYRYG"
     gene            complement(4871..5596)
                     /locus_tag="VG754_10660"
     CDS             complement(4871..5596)
                     /locus_tag="VG754_10660"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWF19722.1"
                     /translation="MARAVHWNRLGYERELWQQGTTLVAGVDEAGCGPLAGPVVAGAV
                     VFPHSWLTTGLYSKLRGLNDSKLLDEEQREKYFNIITKHAEIRYAVAIVDVEMIDRIN
                     IRQAAWRAMNLALDQLQPSPQHVLVDGLKIKWLPYRQTALVQGDGRSYSIAAASVIAK
                     VTRDRLMRELDKQFPGYGFAEHKGYATPQHYAAITAQGPCAIHRKSFSPFRPIAEEME
                     LFDVRAANVAQTNSENASDRQVQ"
     gene            5789..>6044
                     /locus_tag="VG754_10665"
     CDS             5789..>6044
                     /locus_tag="VG754_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19723.1"
                     /translation="MTKLRITDIAGPQRKGAGRPYNVIAPAHQIMIAFFIHRGKKGVP
                     VFSPVRGFVKTRVVAEQENFTRRADEPGNVIEGVHVHIVVE"
BASE COUNT         1389 a         1680 c         1536 g         1439 t
ORIGIN      
        1 ctcggcgcag ccgcgaatgt gctgacggtt cttcgcgcca cccgcgaaag tcactgcccc
       61 cttcgcattc tcagcttcga ccatacgttg gaaccgttgc agtttgcgtt cgaaaatgcc
      121 ataacgcttg gttacttcgg cggctacgaa tacatcgtcg aaacattttt gcgcgagtat
      181 caagtcacct tcatggatgc aaaccgtcaa atcacttggg aattattcct cggcgatttc
      241 ccttctttaa tccgcactcc gcactccgca ctccgcactt cctctcctca ttccatcttg
      301 ttcgatgctt tttcgcccgc gaaaaatccc gccatgtgga cgcagccatt gttcgccgat
      361 ctttttcggc tgctcgatcc cgcgcggcca agcgcgtggc cgacttattc gcgcagcacc
      421 atgctgcggg tttcgttgtt gctcgccggt ttttatgtag gcgtcggtca cgccactgga
      481 gaaaaggacg aaaccaccat cgcggcgaac acgctcgaat tgctcaccga accgctcgat
      541 cacaaatggc ttcaacgcgc ccgcaattcc accagcgccg aaccgatgtg ggaaccggtt
      601 taccggcagg cgcccttgag cgcagaatca tgggacaaac tgcaacaaca tccgcagttc
      661 acgtccttgt ccaaaacggg taaaaaatgc tgatgcaatt tttagtgcgg gccatttcca
      721 cgcgcagttt gaagcttgtc caaaaaatcc ggaccactcc cgttcttttg tttgccgcaa
      781 tcgctttgat gcaattcagc gcatttggag acgaaccaca ggaaccattg ccgttcacag
      841 gcgtgttcac caacggcagc gacggttatc cggttttccg cattccagcg attgtaaaaa
      901 cgcatcaagg cactctgctg gcttttgctg aagcccgcgc cgcgagggct gatggttcgc
      961 aaaataaaat cgtgctcaaa cgcagccatg ataagagcgt gacttgggag aaagctcaac
     1021 tgatatggga cgacagcgcg aattcactga acaatccaac agtcgttgtg gaaaaccaaa
     1081 ctggcagaat tgtcctgata ttccagcgct atccgaaggg catttacgaa aacgatgttg
     1141 tgcccgggct cgatggcgag cgcatttgtc gagcgtttgt cacttacagc gatgatgacg
     1201 ggttgacctg ggcaaaacca acggacatca ccgcatcggt gaagcgtccc acagcaacca
     1261 gcatcgcaag cgggccgggc atcggcatcc agcttgcgcg cggcaaacat gctggacgca
     1321 tcattattcc tttcaatcaa gggccgattg cgaccggaaa agtttatgcg gttttcagcg
     1381 atgaccgtgg cgcgacatgg aaatacggcc aaaccgctcc cggtgaatcg aaaggttccg
     1441 caaacgaagt ccagatggtc gaattaagcg acggctccgt cctgttaaac gcccgcaatc
     1501 aacacgggcc gcatgtccgc aaaacagcgg taagccacga tggcggcgaa acgtggtcca
     1561 cacttcagga tgaaccgcaa ctgattgaac cgacttgcca ggccagcctc atccggcatt
     1621 cgacaatcga taatccaatc gaagaaattc tcttgttctc caatcctgca tccaccacag
     1681 cccgcacgaa cggaaccatc cgcctcagcc gtgatgaagg aaaaacgtgg cccatttcgc
     1741 gcgtcatttg tcccgaccgc ttcggctaca gttgccttgt ctcgctggac gacaaaaata
     1801 tcgggtgcct tttcgaacac ggccaaaaga tttgtttttg ccgttttagt ttagattggt
     1861 tgatggatat taaagaaacc gccaaatgat gattccgtca atcaccacac tcgcaggaca
     1921 actggacaat ctattggctg ataacgccaa catccttgcc cttgcgccga tgcaggatgt
     1981 gacggatttg gcgttttgga aattgatggc gcgttacggc ggcgcggatg tttatttcac
     2041 ggaatatttt cgcgttcacg ctgtttcgca cctggaaaaa tggatcctcg attccatcac
     2101 gcaaaatccc acggccaaac cggtcattgc gcagatgatc ggaaacgaca ttccctcgct
     2161 cgtgcgttcg gcgaaggaat tgcagcaata tcccatcgcc gccgtggacc tgaatcttgg
     2221 ctgtccagcg ccgattgttt atcgcaaatg cgccggtggc ggacttctgc gcgaaccgaa
     2281 acgcgtggac tccattctcg gcgcgttgcg cgacgccgtt tccatcaaat tcaccgtcaa
     2341 gacgcgcatc ggttttgatt caccggcggt ctttgacgag ttgctcccga tttttgcgaa
     2401 acattctctc gacctgctca ctgtccatgg ccgcaccgtt caggaaatgt atcgcacgga
     2461 agtccattac gattacatcg cgcgcgccgt cgccgctgtc ccctgccctg tgctcgcgaa
     2521 tggaaatatt tattccgccg ggaaagcgcg tgaagtcatt caactcaccg gcgcgcgtgg
     2581 cctgatgatc ggtcgcggcg caatccgcaa tccgtggctc ttccgccaaa tccgccagct
     2641 tcagcgcggc gaaccgctgt tcatccccac cggttttgaa gtgctcgaat atgtgcgcga
     2701 cctttacgaa accgttcgtc cgccgcacat caaggaatcc gcgcacattc agaagatgaa
     2761 gaaatacatg aactacatcg gccttgggat cgaaccgagc ggcaagttcc ttcatgaaat
     2821 ccgccgcgtc acaacggaaa ccgatttttt ccgcatctgc gagacctatt taagccacgg
     2881 ccagccgatg ccgctcgaac cattttcgct tggcttgaag gagacagatg tcatggcggg
     2941 cgaacatctt tgagcacttt gtattttaaa tctgaaatgc gcattgcaca aatggctcaa
     3001 aaggaggcgg acctggcaag tgtatccgaa agtccgcctc ctcgctgccg gcacgcgagc
     3061 attcacttct gcccagaacc ggcgtgagcg cttatccatc gcgcctggct aaatcggctt
     3121 cctgggccag ctcattaagg aagttaaaga acgactggtc ctgaccttga aacaatccca
     3181 ttgcagtgaa aaaattcact gttgcgactg cgcctgcctg ttgggtcgaa atgggtcgaa
     3241 tcacggaaca ggcaggaagg tctttgttga taaactcgca cggcgtggca aagatttggt
     3301 tcgtgtcatt cggcccggcg ttgtcagtgc catcgggcgg gtttggcaac gcaggcgcga
     3361 gcggcaattg cggaaacaaa acgttgccca tcggatccgt cacaggcaac gcgtttccgg
     3421 ccttgtcctg ccacgtttgg aaatgttgga tttcagttcc gccaatcgcg ccgatgatct
     3481 gtatcacttc cccgctgctc gccttgggca gcatcgattg atagaggttt gagccgccct
     3541 gctcaatcgt cgcaaaatga aagcccgcga cgttcccgat gaattgtatt tcgaatccgc
     3601 tgggaaggag gtttccatcg gcatcaacag gcggcggcag gtcactgtcc gggatgccgg
     3661 gcacgttcac aaggttgacg atttgtggaa acatcgcgcc aaaatccggg ttcatggaac
     3721 tgcgataacg aaaataccaa ctcgtgtcca cggtaagatt catcaagttg gtcaaccgtt
     3781 tggcagtctt gtttgaaccc gtggcctggc tgcttggcag tctgcgaaac ggttcaaggc
     3841 tgacgggttt cgcgcccttg aacatcaggt aggcgttcag aaaggtcgcg tggctcaatt
     3901 catctcgcgt gttcaaatcc acgtacgtgg cttcatcgcc gtccaggatg ttcaaggcga
     3961 gctggaaaga ctcgttgccg agcgcgagtt cgttgtattg ctgccagagg tctgtctcca
     4021 gaatttcggc ggcggcaaga aaccgaagca aggctgcgtc actcttggtc aagttcttat
     4081 tgcggtcttc tgcgcgcgag gagttgccgc tgctcagaag agcgcccgca ggcagcgcca
     4141 ccgcgccggc cattccaagg cgtttcatga atgagcgccg tccgatggcg ccattggttg
     4201 caagtttttt gtacatagtt cacctgtgct acactgtttg tggtttttac tctgttgaac
     4261 cgtcgctgct tctgacgact tgttttgcca tggtcagcag gccaaactgc atcgtcgaag
     4321 gtaagatgaa ctctttaaaa acttgttcca aagtgatgga aaaaatttca aaaaataatt
     4381 gggaacaaaa aagaatttgc gtcagccgta acgatatggc ttggacatcg caaacgtgtt
     4441 cggcaaatgg cggacctcgc gcacttcact ttccttcaaa aggacttcga caatatcgaa
     4501 acggattttt accggcggat ttttaagaag ttttaagtaa tccagagcgg tttgggaaag
     4561 cagccttttc ttgcgcgcgt tcacgtgcgc tgcgggacgc gtccactctt ctgacgaacg
     4621 cgttttgact tccacaaata ccagacaatc ttcgtcgcga aagaccaaat caatttcacc
     4681 gcgctcggaa caataattcg ccgtgagaaa tttcaacccg agccgttgca gatgtttttt
     4741 ggccgcccgc tcgcccaatt cgccatgacg atgcgcaagc ggctgctcct tgtcttctcg
     4801 ccatgctcga atgcgttgga gaaggttcat tttctgcagt tcctgagttc ctgctttcct
     4861 aattattatc tcactgcact tgcctgtcgc tcgcgttttc actattcgtt tgcgccacat
     4921 tcgcggcccg cacatcaaac aattccatct cctccgcaat gggtcgaaat ggcgagaaac
     4981 tttttcgatg aattgcgcac ggaccttgcg cagtgatggc ggcgtaatgt tgcggtgttg
     5041 cgtaaccttt gtgctcggcg aaaccgtatc ccggaaattg tttgtctaac tcgcgcatca
     5101 aacggtcgcg cgtcactttc gcaatcacgc tcgcggcggc gatggaataa ctcctgccat
     5161 cgccctgcac caatgcagtc tgtcgatatg gcagccattt gattttgagc ccgtccacga
     5221 gcacgtgttg cggactcggc tgcaactggt cgagcgcgag attcatggcg cgccacgcgg
     5281 cctgacggat gttgatgcga tcaatcatct ccacgtccac gatggcaacg gcgtatcgga
     5341 tttccgcgtg tttggtgatg atgttgaaat atttttcgcg ctgttcttcg tcgagcaact
     5401 tggaatcgtt cagtccgcgc agcttggaat acaaacccgt tgtaagccaa ctatggggga
     5461 aaaccaccgc acctgcaacg accggtcccg ccaagggacc gcaacctgct tcgtcaacgc
     5521 ccgcaacgag cgtcgtgcct tgctgccaaa gctcgcgctc gtatcccaac cggttccaat
     5581 gcactgcccg cgccataaat tttttccagt tccaacctat atgggtgggt ggatggttac
     5641 aatcgtcatt ggtaaagatg gttgcatggc catgtccacc aaggacgatt tctcgacgaa
     5701 atgttcatcc ctttgaacaa atgaacccgg actagccgtt gcacacagaa aaagatgcct
     5761 cattaatgga ttggtttggc tgtttgatat gacaaaactt cgtatcaccg atatagctgg
     5821 cccacaaaga aaaggtgctg ggcggcccta taatgtaatc gcaccggctc atcaaataat
     5881 gatcgctttt ttcatccatc gcggaaaaaa aggagttcct gtatttagcc cggtaagagg
     5941 ctttgtcaag acacgggtcg ttgctgaaca ggagaatttt acaaggcgcg ccgatgaacc
     6001 cggcaacgtg atcgagggcg ttcatgtaca catcgtcgtc gaaa
//
LOCUS       DASZMM010000441         4482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_755275, whole genome shotgun sequence.
ACCESSION   DASZMM010000441 DASZMM010000000
VERSION     DASZMM010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755275"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..661)
                     /gene="aroQ"
                     /locus_tag="VG754_10670"
     CDS             complement(224..661)
                     /gene="aroQ"
                     /locus_tag="VG754_10670"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWF19724.1"
                     /translation="MMNILFLNGPNLNLLGQREPEVYGRTTLADIEAMVRDRAAKLKV
                     GVDFRQSNIEGELVTWIQQAKGKFDVIVLNAGAYTHTSVALRDAISGTGVPTIEIHLS
                     NVHAREEFRHNSLIAPVCKGSIAGFGPNSYILGLEAAIIVNVR"
     gene            complement(673..2022)
                     /locus_tag="VG754_10675"
     CDS             complement(673..2022)
                     /locus_tag="VG754_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWF19725.1"
                     /translation="MKRAQPQTPEHKQPDRSLQTIFAAVFGGLLGLSLLKFGNPILME
                     KLVDRPTNSWEWLLNPWPVIIGYWLLAGVTVMGLVIARWKTRAPKILVAMPLVWLAWE
                     IIAAMHSESPKLSWPVVGHFLTCVVCFYLGLFSLSRVERMWPFWMGLIAGFFIVFAFG
                     FEQHFGGLEESRKYWLLYVYPTLKEIQPALYKKMTTNRIFSTLFYPNTLAGVILLLLP
                     MTVAVFWSCRQRFTIGARWLLMGLAAIPAFACLYWSGSKGGWLLMLVIGLLAAMYLPI
                     QRRIKTVLIVAVLVLGLAGFFAKYAGFFKKGATSVVARFDYWEAALKTTASKPVFGNG
                     PGTFGEAYKRIKRPESEMSLLTHNDYLEQACDSGVLGFVTFTGFIAGSLVYVWRRGGL
                     QNDWIKLAVWLGVLGWALQSFVEFGLYIPALSWPAFGFLGWLLGRGGNQFDRQPTAS"
     gene            complement(2052..3101)
                     /locus_tag="VG754_10680"
     CDS             complement(2052..3101)
                     /locus_tag="VG754_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19726.1"
                     /translation="MAFADFPEQKQGVELLQRSLERGRLGHAYLFAGHQLSELEGLAR
                     ALAKVLNCQNPVRRNANSPAVDCCDKCPSCRKIDAENHGDIHWVRPESKSRIITVEQM
                     RDLMHEIFLKPNEAEYKVAVIVAADRLNVQAANAFLKTLEEPPAKSVLVLLTTEPQRI
                     LETIVSRCLRLNFAGEGGRVLAPAQMEWLKTFGELASAEQKSLMGRYRLLGALLKKLG
                     DLREEIEKSLTARSPLERFDDVEPEVRERWEKELSAAIEAEYRLQRSDLLAALQWWLR
                     DVWIQTLESAERGVRSAELLNFPELASPGHVAKRISPKQAVENLRVVERTQRLLGSNV
                     QEALALEVGLLKLNL"
     gene            3720..4109
                     /locus_tag="VG754_10685"
     CDS             3720..4109
                     /locus_tag="VG754_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19727.1"
                     /translation="MTRSNVGRIDAHEPVWHVLFVARCCGSSSRAPFGPVNPPVLKSA
                     GASTQLPRSVGSMTRSTVGRIDAHEPVWHILFVACCCGSSSRAPFRPVNTSGNSPPSP
                     VPAWPTNRAFRGYAKTLNIQRRTLNAE"
     gene            complement(4185..>4482)
                     /locus_tag="VG754_10690"
     CDS             complement(4185..>4482)
                     /locus_tag="VG754_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWF19728.1"
                     /translation="ISFAAGTPLSSAVTSLNGATRSGTSLVGSLAPNSLGLYDTRGNV
                     MEFCMNDEAKHFWFLKGGSWADFVEVNLRPDFRWYCKPDDRKNTFGFRCVLKKG"
BASE COUNT         1102 a         1353 c         1109 g          918 t
ORIGIN      
        1 aatccacaaa cccgctttcg tttaggttca ccttccggca ctatttaagc gccaactctc
       61 caactggaga caactcgaca tggactgcgt cacaatgctg tcacatgtgt gggtgggaaa
      121 gtagtaggtg agggaaaatg cgtcaagaga taaaattaac ctctccacac ccggctcaaa
      181 tatgccaata aagcagctaa aaaaacccat tttctcggag tttttatctt acgttaacga
      241 taatagccgc ttctagaccc aaaatgtaag aattcggccc aaagccggct atcgagcctt
      301 tgcaaaccgg ggcaatcaag gaattgtgac ggaattcttc ccgggcatgg acgttcgaga
      361 gatggatttc gatggtcggc accccggttc cagagatggc atcccggagt gcgacgctgg
      421 tatgggtgta ggcaccggcg ttcaggacaa tgacgtcaaa cttgcccttc gcttgttgaa
      481 tccaagtcac cagttccccc tcaatgttgg actggcggaa gtccacccca actttcaatt
      541 tcgccgcacg gtcccgaacc atggcctcga tgtccgccaa ggtcgtccgg ccatagacct
      601 ccggctcccg ctgccctagc aggttcaggt ttggaccgtt tagaaataaa atgttcatca
      661 aggcgacgga gcttaactgg cggttggttg tctgtcgaat tgattcccgc cacgtcccaa
      721 caaccacccc aagaacccaa aagccggcca gctcagggcg gggatgtaca gcccgaattc
      781 cacgaaactc tgcaacgccc agcccaacac ccccaaccat accgccaact tgatccaatc
      841 attttgcaat ccgcctcttc gccagacata gaccaacgag cctgcaataa atcccgtgaa
      901 cgtcacaaat ccgagcacgc ctgaatcgca ggcctgttcc agataatcgt tatgggtcag
      961 cagggacatc tcggattcgg ggcgtttgat gcgcttgtag gcttcgccaa aagttcctgg
     1021 cccattgccg aacacgggct tcgaagccgt tgtcttgaga gcggcttccc aataatcaaa
     1081 ccgggccacc acactggtgg ctcccttctt gaaaaaaccg gcatacttcg caaaaaaccc
     1141 cgcgaggccg agaaccaaca ccgcgacaat cagaaccgtc tttatccgcc gttgaatcgg
     1201 cagatacatg gccgccaaca atccgatcac cagcatcagc agccatccgc cttttgaccc
     1261 cgaccaatag aggcaggcga atgccgggat ggctgccaag cccatcaaca accaccgcgc
     1321 cccgatcgtg aagcgttgac gacaggacca aaagaccgca actgtcatcg gcagcagcaa
     1381 caaaataaca cccgccaaag tattcggata aaacaatgtc gaaaaaatcc ggttggtggt
     1441 cattttctta taaagagccg gctggatttc cttgagagtc ggatagacat acaagagcca
     1501 atacttccgc gattcctcca acccgccaaa atgctgctca aaaccaaacg cgaaaacgat
     1561 gaaaaaccct gcgatcaatc ccatccaaaa cggccacatc cgctcgaccc ggctcaatga
     1621 gaacaaaccg agataaaagc aaaccacgca ggtcaaaaaa tgtcccacca ccggccagct
     1681 taattttggc gactctgaat gcatcgccgc gatgatttcc catgccagcc agacgagcgg
     1741 catcgcgacc aggattttcg gcgccctggt tttccagcgg gcaatcacca atcccatcac
     1801 cgttactccc gccagcagcc agtaaccgat gatcaccggc catggattca gcagccattc
     1861 ccacgaattg gttggacgat caacgagctt ctccatgaga attggattgc cgaatttcag
     1921 cagcgacaat ccgagcaagc cgccgaacac tgccgcaaag atcgtctgca acgaacgatc
     1981 cggctgcttg tgttccggcg tctgcggctg tgcgcgtttc attttcccgc tggaggcgac
     2041 gcgtgtcaca atcacaaatt cagcttcaac aagccgactt ccaatgccag cgcttcctgc
     2101 acattcgagc caagcaatct ttgcgtccgt tccacgaccc gcagattttc caccgcttgc
     2161 ttcggggaaa ttcgtttggc gacatgcccg gggcttgcca gctctggaaa gttcagcaac
     2221 tccgcgctcc gcactccgcg ttccgcgctt tcgagcgtct ggatccagac atcgcgcagc
     2281 caccattgca acgccgccag caggtccgac cgttgcagcc ggtattccgc ttcgatggcc
     2341 gcgctcaatt ccttttccca tcgctcgcgc acttcgggtt caacgtcgtc aaaccgttcc
     2401 aatggcgaac gcgccgtcaa cgatttctcg atctcctcgc gcaagtctcc cagctttttc
     2461 aacaacgcgc ccagcagccg gtaacggccc atcaaactct tttgttccgc ggacgccaat
     2521 tcgccgaaag ttttcaacca ttccatctgc gccggtgcga gaacacgtcc gccttcgccg
     2581 gcaaaattca gccgcaaaca acgcgacaca atcgtttcca ggatgcgttg cggttccgtg
     2641 gtcagaagga ccaggacgga ttttgccggt ggttcctcca atgttttaag aaacgcattg
     2701 gccgcctgca cgttcaaacg gtccgccgca acaatcacgg ccaccttgta ttccgcctcg
     2761 ttcggtttca ggaaaatctc gtgcatcagg tcgcgcatct gttccacggt gatgatccgc
     2821 gacttggatt ccggccgcac ccaatggatg tcgccgtgat tctccgcgtc aatcttgcgg
     2881 cagcttgggc atttgtcgca gcaatccacc gccgggctgt tcgcgttgcg ccggacgggg
     2941 ttttggcaat tcaacacttt cgccagcgcg cgcgccagcc cttccaactc cgacaattga
     3001 tgcccggcga acaggtaggc atgaccgagc cgcccgcgct caagactgcg ctgcagcaac
     3061 tcgacgccct gcttctgttc cggaaaatcc gcgaatgcca tataagtata taattccatc
     3121 agccgggcca agtcaccaac caagtgaggg ccatccggac ccatgagacg cttgaaaaaa
     3181 attaaaattt ttggcggaaa caagcggaat ggggtggaaa caagcggaaa caagcggaaa
     3241 tttctccgga tagattgcgg aaattggcaa gtcacaagcg gaaaaccctg gtttgtggac
     3301 atcggttgaa cggaccctcg ccgccagttc ttcggagcgc ccgccttggc ccggcaagat
     3361 tcgcgaataa aaaaatgttt ttccgttatc gttaacaact gaaaatagct gaaaataact
     3421 gaaaatagct gaaagtattt gaaacaagct gaaaacttgg gcttgccatt gctgaaaata
     3481 ttggattgat cgcaaagacg cgagacacag agatatgaag gcggtgagat ggccggcgcg
     3541 tgtataaagg aactcacttc cggcgaaaaa tggccggtga cgtcggcatc gggcgatgcc
     3601 atctggtgct ccttggcgca cgccaaagat ggtttcataa tccgcgaaaa ttgtccagaa
     3661 gaatccaccc gcgctcaaga gtgcgggcgc atccgcgcac tgccccggag cgcgggtcga
     3721 tgacccgcag caatgtggga aggatagacg cgcatgaacc agtctggcat gtcctgtttg
     3781 tcgcgcgttg ctgcggctca tcgagccgcg ctccgtttgg tccggtgaat ccacccgtgc
     3841 tcaagagtgc gggcgcatcc acgcaactgc cccggagcgt cgggtcgatg acccgcagca
     3901 ctgtgggaag gatagacgcg catgaaccag tctggcatat cctgtttgtc gcgtgttgct
     3961 gcggctcatc gagccgcgct ccgtttcgtc cggtgaacac atcgggcaat agcccaccca
     4021 gtcccgtccc ggcatggcca acaaatcggg catttcgcgg atacgcaaaa acgctgaaca
     4081 tccaacgccg aacgttgaac gccgaatgaa actgccacaa cggcaggaat tggacgttca
     4141 gcgttcggcg ttggatgttc gatgtttctt tttcaacact ccgcctatcc ctttttcaac
     4201 acacaccgaa acccgaaagt gttcttgcgg tcgtccggct tgcaatacca gcggaagtca
     4261 gggcgcagat tcacttccac aaaatcggcc cacgaaccgc ctttcagaaa ccaaaaatgc
     4321 ttggcttcat cgttcatgca gaactccatc acattgccgc gcgtgtcata aagtccaagg
     4381 ctgtttggag ccaggctgcc gacaaggctg gttcccgaac gggtcgcgcc attcagactt
     4441 gtgacggcgc tggacaatgg cgtgcccgcc gcgaaagaaa tc
//
LOCUS       DASZMM010000442         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4036865, whole genome shotgun sequence.
ACCESSION   DASZMM010000442 DASZMM010000000
VERSION     DASZMM010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4036865"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VG754_10695"
     CDS             <1..864
                     /locus_tag="VG754_10695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWF19729.1"
                     /translation="VPVTKDTVFEIGSITKQFTAACVLLLAQEGKLSLDDKINDHLKV
                     APTNWSNITVRHLLTHTSGLPNYTLLDGFELRSHLTQEQFIQKLSAEPVIFQPGDSWS
                     YCNSGYSLLGYIVENVSGQPYWDFLHRRILDPLGMTSTRDRNPGVIIKHRAAGYEMKN
                     NQPINRDYDLTDIFSAGAIVSSVVDMAKWDAALNGETLLNQSSKDSWWTPTKLNNGKV
                     QNYGFGWYVDALKGHKNIGHGGATSGFSASIQRFPDDHFSVIVLCNANELNVASVIAK
                     EVAELYFEKHP"
     gene            complement(868..1341)
                     /locus_tag="VG754_10700"
     CDS             complement(868..1341)
                     /locus_tag="VG754_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HWF19730.1"
                     /translation="MSSRAGRVTPVMMQIEKLIEFRDSPIHGTGGFATVDIRADARVV
                     EYLGRKIDKQESLRQCELENPYVFCLDDEFDLDGNVDWNPARLLNHSCSPNAEAQADG
                     ERIWIVALRDIRAGEEITFNYNYDLQDYREHPCVCGATNCVGYIVAEEFFSQLSK"
     gene            1533..1775
                     /locus_tag="VG754_10705"
     CDS             1533..1775
                     /locus_tag="VG754_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19731.1"
                     /translation="MPAKVQHTILAYGGSAEIKDINTVGEDEDVYKVQFTNPGVTPTL
                     FVSEDGTPLNAAPTNSVPWVEPPPVPSTLDQRKTSL"
     gene            complement(1791..>2679)
                     /gene="rsmH"
                     /locus_tag="VG754_10710"
     CDS             complement(1791..>2679)
                     /gene="rsmH"
                     /locus_tag="VG754_10710"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWF19732.1"
                     /translation="RPICVREMMELLGPKPGDLAVDATLGHGGHALEVLRAILPGGRL
                     LAMDADPLELPRAEARLRATGIPTEALVVRRSNFAGLPQFLASEGIAGVDIVLADLGV
                     SSMQIDNPERGFSFKHDGPLDLRMNPQRGQPASAFLASVGEAELAGILAEYADEPRAA
                     LIAQKICQSRAVAPIVTTKNLARIVHDALGRFRLSKAADDSVRRVFQALRIAVNDEFG
                     ALETFLRNLPHCLKPNGRVAILTFHSGEDRRVKRAFQAGERDGIYSSVAHEVIRPSPE
                     EVRANPRASSAKLRWAIRA"
BASE COUNT          631 a          774 c          721 g          553 t
ORIGIN      
        1 gtgccggtga cgaaagacac tgtgtttgaa atcggctcca tcaccaaaca attcaccgcc
       61 gcttgcgttc tgctgctggc gcaggaagga aaactttcgc tcgacgacaa aatcaatgac
      121 catctgaaag tcgccccaac aaattggtcc aacatcaccg tgcgccatct gctgacgcac
      181 acctccggcc tgccgaatta tactttgctg gatggatttg aattgcggag ccatctcacg
      241 caggagcaat tcatccagaa actcagcgcc gagcccgtga tttttcaacc gggcgattcg
      301 tggtcttatt gcaactccgg ctacagcctg ctcggttaca tcgtcgaaaa cgtcagcggc
      361 cagccgtact gggatttttt gcatcgcagg attctcgatc cgcttgggat gacttcgacg
      421 cgcgaccgca acccgggagt catcatcaaa caccgcgctg ccggttacga gatgaagaac
      481 aaccagccca tcaatcgcga ttatgatttg accgatattt tttccgcggg cgcaatcgtt
      541 tcgagcgtcg tggacatggc gaaatgggac gccgccctca atggtgaaac attgctcaat
      601 caatcaagca aagactcttg gtggacgccg acaaagctca acaatggcaa agtgcagaac
      661 tacggcttcg gctggtacgt ggatgccttg aaaggccaca aaaacatcgg ccacggcggc
      721 gcaacctcag gattttccgc ttcgatacag cgttttccag acgaccattt cagcgtgatc
      781 gtgttgtgca acgcgaatga attgaacgtc gcgagcgtca tcgcaaagga agtcgctgaa
      841 ttgtattttg aaaagcatcc ttgaggctca cttggagagt tgagaaaaaa attcctctgc
      901 cacgatgtag ccgacacaat tggtcgcgcc gcagacgcaa ggatgttcgc gataatcctg
      961 caggtcgtaa ttgtagttga acgtgatctc ttcgccggcg cggatgtcgc gcaacgccac
     1021 aatccagatg cgttcaccat cggcctgcgc ttcggcgttc ggcgagcaac tgtggttcaa
     1081 tagccgagcg ggattccaat ccacgttgcc gtccaggtcg aattcatcat cgaggcaaaa
     1141 aacgtaaggg ttttccagtt cgcattggcg cagcgattcc tgcttgtcga ttttccggcc
     1201 caaatattcc accacacgcg cgtcggcgcg gatatccaca gttgcaaaac caccggtgcc
     1261 atggatgggc gaatcgcgaa actcaatcag cttttcaatc tgcatcatca caggagtaac
     1321 acgcccggcg cgcgaactca acaatcaggt caaatcccca ttggaaagca cggtcgaaca
     1381 gcggaaattg gcgtgtgaaa acctcagttg tcggacccgt tatgattttg gtcgcgctaa
     1441 cgggatgcgt aatctgcgcg aagcacccgg acgttcatgc taaaattgtt cctcccgtgc
     1501 agttggtcag tccagggacc aaatttggtc ttttgcctgc gaaggtgcag cataccattc
     1561 tcgcttacgg cggctcggcg gaaatcaagg acatcaatac agtcggcgag gatgaagacg
     1621 tatataaagt ccaatttacg aatcccggcg tgactcccac cctttttgtc tcggaagatg
     1681 gaacgccgct gaatgccgca cccaccaact ccgtgccctg ggttgagccg ccgccagtcc
     1741 catcaacgct cgaccaacgc aagaccagcc tctgacgaat cgtatttcgt tcaggcacga
     1801 attgcccaac gtaacttggc gctcgacgcg cgcggatttg cgcgaacttc ctccggactc
     1861 ggacgaatca cttcgtgtgc cacgctggaa taaatgccgt cgcgttcgcc ggcttgaaac
     1921 gctcttttca cccgccggtc ttcgccggaa tgaaaagtca ggatggcaac ccggccattc
     1981 ggcttgagac agtggggaag gttgcggaga aaagtttcca acgcgccgaa ctcgtcattg
     2041 acggcgatgc gcaaggcttg aaagacacgg cgcacactgt catccgcggc tttcgagagc
     2101 cggaagcggc caagggcatc atgcacgatt cgcgcaaggt ttttggtggt gacgattggc
     2161 gcaaccgcac gtgactggca aattttttgc gctatcaaag cggcgcgagg ttcgtccgca
     2221 tactccgcca ggattcccgc aagctccgct tcgccgacgc ttgcaagaaa tgcggacgcc
     2281 ggttggccgc gctgagggtt catgcgcaaa tccagcggcc catcatgctt gaaagaaaaa
     2341 ccgcgctcag gattgtcaat ctgcatcgag gaaacgccaa ggtccgcgag cacgatgtcc
     2401 actccggcga ttccttctga ggcgagaaat tgcggcagtc cggcgaaatt cgaacggcgc
     2461 acgacgagtg cttcggtcgg aatgcccgtc gcgcgaagcc gtgcctcagc gcgcggcaat
     2521 tccaacgggt ccgcatccat ggcaaggaga cgtccgccgg gcaggatggc gcgaaggacc
     2581 tcaagagcgt gcccgccgtg acccaaagtc gcatcaaccg ccagatcgcc gggctttggc
     2641 ccgagcaact ccatcatttc gcgcacgcaa atcggccga
//
LOCUS       DASZMM010000443         6303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2395008, whole genome shotgun sequence.
ACCESSION   DASZMM010000443 DASZMM010000000
VERSION     DASZMM010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395008"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..3736)
                     /locus_tag="VG754_10715"
     CDS             complement(146..3736)
                     /locus_tag="VG754_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HWF19733.1"
                     /translation="MSALSHSLIQRSLVGHLGGTGLAGVLACKLSPLFLLLFFFLPGF
                     AAPIAVTGQWDFKYGDLRATVGADLQYINDTSNITTFGVMKVVGGLATVMGYGSNSPS
                     QGFYMRHGAKPNGGGQFVNQYTLIMDIMSPGGAGNDSSIWTPLIQANTFNSPGTDAQF
                     YFGTTSTPGPDGLGPSGQMDASLAAYTWYRLGLVVDLTAPVGHQLTKYLNGMMVGAQN
                     LSGGIDGPYALGPTALLFTTGLSGSGPAIQPGYVNSIQFINGCMSSNAMAALGVPAPD
                     GIPSNDTQPLIQVSKGSWQSPSTKFTWLGRSSSTLQLQIASSLNSPNWQTIGTLSSVN
                     SLTMPSSVMSNSSGFFRVAGPLANTAQAGVATEPDIVVGRQPTGEQSVPTKQLLRTPG
                     QSVIFSGRPTDLVMSPNGSSVFVKNNTSLLVVNAASWRVIQTLKFPANQGSMHGIAVN
                     PAGTHVYVTGTAQELYDYTVSGGSVAYSQTINVPASSNPNGIAISADGSTAYVCLTSS
                     NFLAAVNLSTTNIQLIKVGVAPYDVVLSSDGKTACVSDWGGRQPQAGDNVSWSLNSQI
                     VVDNRGIAASGAVSIVDLTQNLEVTEVPTGLHPNAQALSPDGTTLYVANANSDTVTVI
                     NVQARTVQETISMRPNSALPFGSASDGLALSPDGTTLYVANAGNNAIGIVQLPNSQHT
                     NSVVQGFVPTDWYPGAVLADATNLYVVNVKGLGSGSGTVTQLNGSADKIPLPSQTVLS
                     KYTAQVQENGRVPQMLQAQLPAQSGAIPLPVPAHVGEPSVFQHVLYVIKENKTYDQVL
                     GDMAQGNGNSNLCIYGQFVTPNHHALASQFVLLDNYYCNGVFSTDGHSWCTEANSTDY
                     WEKSINAINRAGHQGTDPLIYSSSGFIWNNVLQHNLTFHNYGVMGGSSAHPGNPTWLQ
                     VYGDYTNHTGAISYSTGIGCPPTLPQYSSTNVSGFNLSIPDQLRADGWLAEFNAAQSS
                     GVWPAFNMLYLPNDHTAGGAPNYPTPRAQVADNDLALGRVVDAVSKSVFWTNTVIFVI
                     EDDPQSGYDHVDGHRSLCLVISPYTRRGQTVSTFYNQVGMIHTMEQILGLAPMTQMDA
                     MGPLMTDCFTNTPNLAPYNFVANNVALNEMNPGTTGALSRKERYWARMSQKMDLSKPD
                     QVNDDLFNRILWHSAKGDARYPAEFVGTHGKALAKLGLVASKPGKDQDDD"
     gene            4230..>6303
                     /locus_tag="VG754_10720"
     CDS             4230..>6303
                     /locus_tag="VG754_10720"
                     /inference="COORDINATES: protein motif:HMM:NF020044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HWF19734.1"
                     /translation="MEKVELTDALFAKLAGWEAVKAARAMIAAGRVLSSDWEAPRLIG
                     KVQEGSTAINTGLIIRSASDAENLCPCRDSRQRGLICAHAVAVGLHHIKSNQLPSAPV
                     EPKHAKVQISKRPVKTLRRGSGADGQPLELFLILPPNFIEALRKGRVMLYVEGQWPKG
                     RSPLNALSYDVPFTMSAQDAALLDFLESLNEGDTPAMLMLNSSQFAELLQKIIGHPRV
                     TFGRTQFAEILGEPLKLAVKVTLEKNGEIVLRLSNSVPSGIIPGATTWVLQERTFRPV
                     GLPLAFGQLFNGPMRIARTKVPQFLNLELPTLKSSCDAYVDFEIGDFEMESATPRFKL
                     HLAGGLAVLEAKLECSYGDKSVTPGSFAQTNAFWLPDPESPTRYWTRNLELEQAAVAR
                     LMKNGFSGPDAQGLRHLKGQNPVLNFFAREYPRLEKEWDVTLEERLERSTQQNLERIE
                     PKFQITPSGEEWFNLNVSYDTRGGEKFSSADIQRLLLSGQSHSPLKNGKFALLDTGAV
                     EELQEVLLDCAPEQHPGGYRMDNAQAGFLDATFRGQPGWQVRAPEAWAQRAAQQSGEV
                     KVTSPPLGALESVLRPYQKEGVAWLAFLRANGFGGILADEMGLGKTLQVLALLQSCTE
                     RKNTAAPSLVVCPTSLVFNWTAEAARFTPELRVLALHGPRRHEQFAQIPDHDLIITSY
                     ALLRRDAAT"
BASE COUNT         1407 a         1699 c         1773 g         1424 t
ORIGIN      
        1 aatggacgcc aatgggtttg tgggatttgg aaaatcatga ttcaaaattg catcgcaaga
       61 tccatcagcc aatggctgaa gttttgcgaa cggcgcaaca aaaactcaat gccgcaaagt
      121 caatcaagcc cgttgcggcg ctggcttaat cgtcgtcctg gtccttgcct ggtttggagg
      181 cgaccaaacc aagttttgcc aacgccttgc catgtgtgcc cacgaactcc gcaggataac
      241 gcgcatcgcc cttggccgag tgccaaagga tgcgattgaa caaatcatcg ttgacttgat
      301 ccggcttgct caaatccatt ttctggctca tccgcgccca ataacgttct ttgcggctca
      361 atgctcctgt tgtgccggga ttcatctcgt tcagggcgac gttgttcgcg acaaaattgt
      421 aaggagcaag attcggcgtg ttggtaaaac agtcggtcat caacgggccc atggcgtcca
      481 tctgggtcat cggagccagt ccaaggattt gttccatcgt gtgaatcatt cccacctgat
      541 tgtagaaagt gctgacggtc tggccgcgcc ttgtgtaggg gctgatgaca aggcaaagag
      601 agcgatggcc atccacatgg tcgtagccgc tctgcggatc gtcctcgatc acgaaaataa
      661 ccgtgttcgt ccagaaaaca cttttgctga ccgcatcaac gacgcgaccc aaggcaaggt
      721 cgttgtcggc gacctgcgcg cgtggtgtgg gataattcgg cgcgccgcca gcagtgtgat
      781 cattcggcaa atacaacata ttaaacgcgg gccagacacc actggattgc gcggcattga
      841 attccgcgag ccaaccatcg gcgcggagtt gatcaggaat gctgagattg aaaccggaaa
      901 cattcgtcga actgtattgc ggcaaagtcg gagggcaacc aatgcctgtg gaataggaga
      961 ttgcgccggt atggttcgtg taatccccat agacctgcag ccaggttgga ttgccaggat
     1021 gggcggagga accgcccatc acgccataat tatggaaggt caggttatgc tggagcacat
     1081 tgttccagat gaatccgctc gaggaataaa tcaatgggtc ggttccttga tgccctgcgc
     1141 ggttgatggc gttgatggat ttttcccagt agtcggtgga gttcgcttct gtgcaccagg
     1201 aatgcccgtc cgtcgaaaaa acgccgttgc aataataatt atcgagcaac acgaattgcg
     1261 atgcgagggc gtgatgattg ggtgtgacga attgcccgta gatgcacaaa ttggaattcc
     1321 cgttcccctg ggccatgtct cccaacacct ggtcgtaggt cttgttttcc ttgatgacgt
     1381 acagcacgtg ctggaatacg gagggctcgc caacatgagc gggcacgggc aaggggatcg
     1441 cgccggattg cgcgggcagt tgtgcctgga gcatctgcgg cacacggccg ttttcctgga
     1501 cttgcgcggt gtatttgctc aacacagtct ggcttggcaa tggaatcttg tccgcgctcc
     1561 cgttcaattg tgtgaccgtt ccacttcccg aacccagacc cttgacattg accacataca
     1621 gattggtcgc atccgccagc accgcgccgg gataccaatc ggtcggcacg aatccctgca
     1681 ccacactgtt cgtgtgctgg ctgttgggca attgcacgat gccgatggcg ttgttgccgg
     1741 cattggcaac gtaaagtgtc gttccatccg ggctcaaagc caatccatcg ctggcgctgc
     1801 caaaaggcaa cgccgaattg ggccgcatcg aaatcgtttc ctggaccgtg cgggcttgga
     1861 cattgatgac cgtcacggta tcggaattcg cgttggcaac atacaaggtc gttccgtcgg
     1921 gactcaacgc ctgagcgttg ggatgaaggc cggtcggcac ttcggtgact tcaagattct
     1981 gtgtcaaatc cacaatcgaa acggcgccgc tcgcagcaat gccgcgattg tccaccacga
     2041 tctgcgaatt gagagaccac gaaacattgt cacccgcctg cggttggcgt ccgccccaat
     2101 ccgagacgca ggccgttttt ccatcgctgg aaagcaccac gtcgtacggc gcaacaccca
     2161 cctttataag ttggatgttc gtggtggaaa gattgacggc ggcaaggaag ttcgagcttg
     2221 tcaggcaaac atacgcggtc gaaccgtccg ctgaaattgc gatgccgttc ggatttgagc
     2281 ttgccggcac attgatggtt tgtgaataag caaccgaacc gccgctcacc gtataatcat
     2341 acaattcctg cgctgtgccg gtgacataca catgcgttcc cgcaggattg actgcgatcc
     2401 cgtgcatcga gccctggttg gccggaaatt tcaaagtttg aatcacacgc catgaggccg
     2461 cattcaccac cagcaaagaa gtgttgtttt tgacgaaaac cgaacttcca ttcggcgaca
     2521 tcacaaggtc cgtcggtcgc ccgctaaaaa taaccgactg ccccggagtg cggagcagtt
     2581 gcttggtggg aacagattgt tcgccggtcg gttgacggcc gaccacgatg tccggctcgg
     2641 tcgccacgcc tgcctgggcg gtgtttgcaa gcggcccggc aacacggaaa aaaccggacg
     2701 aattgctcat caccgacgag ggcattgtaa ggctgttgac gctgcttaac gtgccgatcg
     2761 tttgccagtt tggactgttg agactggacg cgatctgcaa ttgcaatgtt gaactgctgc
     2821 gcccaagcca cgtgaatttg gtcgaagggc tttgccacga gcctttcgat acctgtatca
     2881 aaggctgggt gtcattggat ggaatgccat cgggagcggg gacgcccaac gccgccattg
     2941 cattggatga catgcagccg ttgatgaact ggatgctgtt gacgtaaccc ggctggatgg
     3001 caggtccgga accgctgagg cctgtggtga aaagcagcgc tgtcggtccc aaagcgtatg
     3061 gaccatcaat gccgccagac aggttttgcg cgcctaccat catcccgttg agatatttcg
     3121 tgagttgatg gccgacagga gcggtcaaat ccacgaccag ccccagtcga taccaagtgt
     3181 aagcggccaa agaagcatcc atctggccgg atggtcccaa gccatcgggt cccggcgtgc
     3241 tggtggtccc gaaataaaat tgtgcgtcgg tgcccgggga attgaacgtg tttgcctgta
     3301 tcaggggcgt ccaaatgctt gaatcgttgc ccgccccgcc cggagacatg atgtccatga
     3361 tgagcgtgta ttgattgaca aattgtccgc cgccattcgg ctttgcgcca tgccgcatgt
     3421 aaaagccctg gcttggcgaa ttgctgccgt aacccatcac tgtggccaat ccgccgacca
     3481 ccttcatcac gccgaacgtc gtgatattgg aagtgtcgtt gatgtattgc aaatcggccc
     3541 cgacggtcgc gcgcaaatcg ccatatttga agtcccattg gccggtcact gcgatcggcg
     3601 ccgcaaatcc cggcaagaaa aagaacagca gaagaaacaa aggcgaaagc ttgcaagcaa
     3661 gcacgcctgc caaacctgtt cctcccagat gaccgacaag gctcctctgg atcagtgaat
     3721 gtgacagcgc gctcatggct ataacttctg gaatacgagc gaaattttgg ctgaatgcgt
     3781 ccacctcgga tatggggtat atgcgaaacc tcggtttctt tttgtcctac aaaactgtcg
     3841 ggttcacaga gagtggtcgc caactgcgcc gcttaacttt ttcaggaggt tggaaaaatc
     3901 gggccggcag cgccatcggg tactttcgcc gatgaaagcg tgcgcaaaac agttgcatct
     3961 gccacgtcct cattctggta tggaaacatt attccgctgt ctccctcatt tggcaagtcg
     4021 taagcggcac gaccgtctcg ctgtcgtttt ttgaccatca aacaaattta tttttctccc
     4081 gaatatgcgg tataaggcga caaggtcgca tcgtaatact ggcatgagac gtgggacaca
     4141 aagtgtccaa gaccaacgaa aatgtgccat gcgtctcatc cgcgcttttc actttggctt
     4201 gcggcggttc tgcggcaaag tgattgaaga tggaaaaagt cgaattaacc gacgcgctgt
     4261 tcgcaaagct ggccggttgg gaagcggtga aggcggcgcg cgcgatgatt gccgcagggc
     4321 gtgtgctcag ttcggactgg gaggcgccgc ggttgattgg aaaggtgcag gaaggttcga
     4381 cggccatcaa taccggtctc atcattcgga gcgcttcaga tgccgagaat ctttgtccct
     4441 gccgtgattc ccgccagcgc ggattgattt gcgcccacgc agttgctgtt gggcttcatc
     4501 atatcaagtc caatcaactc ccttctgcgc ctgtcgaacc gaaacatgcc aaagtgcaaa
     4561 tttcaaagcg tccggtaaaa accttgcggc gcggctccgg cgctgatggc caaccgttgg
     4621 aattgttcct gattttgccg cccaatttta tcgaagcctt aagaaagggc cgcgtcatgt
     4681 tgtatgtcga aggccaatgg cccaaaggcc gttcgccgtt gaatgcgttg tcatacgatg
     4741 tcccgttcac gatgtcagcg caggacgccg cgttgctcga ttttttggaa tcgctgaatg
     4801 aaggcgatac tcccgcgatg ctgatgttga attcaagcca gttcgccgaa ctgcttcaaa
     4861 aaatcatcgg gcatccccgc gtcacatttg gccggacaca atttgcggaa attctcggcg
     4921 agccgctcaa gctcgccgtg aaagtgacac ttgaaaagaa cggtgaaatc gtcctgcgcc
     4981 tctccaactc cgtgccatca ggaatcattc ccggcgcaac gacttgggtt ttgcaggaac
     5041 ggacgtttcg tccggtggga ctgcctttgg ctttcgggca attattcaac ggcccgatgc
     5101 gcatagcgcg aacgaaggtg ccgcagtttc tgaatttgga attaccgacg ctcaagtcaa
     5161 gttgcgatgc gtacgtggat tttgagattg gcgattttga aatggaatcg gccacaccgc
     5221 gtttcaaatt gcatcttgcg ggtggactgg cggtgctgga ggcaaaattg gaatgctctt
     5281 atggcgataa atctgtcacg ccgggaagtt tcgctcagac caacgcgttc tggctgcccg
     5341 acccggaatc gccgacgcgt tattggacgc ggaatttgga gttggaacaa gccgcagttg
     5401 cacggttgat gaaaaacggt ttcagcggcc ctgatgcgca aggcttgcga catttaaaag
     5461 gccaaaatcc ggtgctgaat ttttttgccc gcgaatatcc gcgccttgaa aaagagtggg
     5521 acgtgaccct ggaggaacgt ctcgaacgaa gcacgcaaca aaacttggag cgtatcgaac
     5581 cgaaattcca gatcacaccc tccggtgagg aatggttcaa tctcaacgtc tcctacgaca
     5641 cgcgcggcgg cgaaaagttt tcttccgccg acatccagcg tttgttgctc tccggccaaa
     5701 gccattcgcc cctgaaaaac ggcaagttcg cgttgctcga caccggcgcg gtggaagagc
     5761 ttcaggaagt tttgctcgat tgcgcgccgg aacagcatcc cggcggttat cggatggaca
     5821 acgcgcaagc cggttttctt gacgccacat tccgcggtca acccggctgg caagtccgtg
     5881 cgccggaagc atgggcccaa cgcgcggcgc aacaatccgg cgaggtcaaa gtcacttcgc
     5941 cgcccttggg cgcgcttgaa tccgtcttgc gcccgtatca aaaggaaggt gttgcctggc
     6001 ttgcctttct tcgcgcgaac ggtttcggcg gcattttggc cgatgaaatg gggcttggca
     6061 aaactttgca agtgctcgcg ctgcttcagt cgtgcacaga aagaaaaaac acggctgctc
     6121 cgtcgcttgt ggtttgtccg acttcgctgg tctttaattg gactgcggaa gccgcgcggt
     6181 tcactcccga attgcgcgtg ctggcgcttc acggcccgcg gcggcatgaa caattcgcgc
     6241 agattccaga tcacgatttg atcatcacga gctacgcgct gcttcgacgc gacgccgcaa
     6301 ctt
//
LOCUS       DASZMM010000444         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_996728, whole genome shotgun sequence.
ACCESSION   DASZMM010000444 DASZMM010000000
VERSION     DASZMM010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996728"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VG754_10725"
     CDS             <1..958
                     /locus_tag="VG754_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF19735.1"
                     /translation="IFMTLGGIAPHLLGYSHPLLLLELAIVVAWTLSMCFTWPSLQAL
                     LSHAQSPTELPHTAGIYNMVWAGASALAYLTSGALLDHFGGEILFWVPAGLHVLQLVL
                     LPRLQKMDSSLPVEEPAQSGESLPELNPRPIARARTFVKLAWIANPFAYMAIYAVIPI
                     IPRLAAQFNLTPTYAGLVCSIWFWARVAAFAGLWLWPGWHYRFRWLLAAFLGLIASYV
                     AIPLSANLWMLVAAQVVFGLVVGLIYYSSLFYSMDVGESRGKRGGFHEAAIGMGIFVG
                     PAAGVGALQAFPGQPNAGTWAISGLLVIGLGLFLWTQFREQR"
     gene            1184..1954
                     /locus_tag="VG754_10730"
     CDS             1184..1954
                     /locus_tag="VG754_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HWF19736.1"
                     /translation="MPELSGKLGIVFGVANKRSIAWAIAQAWHKAGARLAFTYQGERL
                     KENVEELAGTFGADTLLMPCDVTKDEEIARVFSEVGEKFGKLHLLLHSVAFAPKEALE
                     GHFINTSREAFRIAHDVSAYSLIALARGAAPLMTEGGSIVGMTYYGSEKVVPHYNVMG
                     VAKAALEASTRYLAYDLGPKKIRVNCISAGPVNTLAARGIAGFTEMLKHYEAHAPLKR
                     NVLPDELGATGTFLATDGAAAITGQVLYVDCGYQIMGM"
     gene            complement(2018..2416)
                     /locus_tag="VG754_10735"
     CDS             complement(2018..2416)
                     /locus_tag="VG754_10735"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HWF19737.1"
                     /translation="MHRVKFLRSQFKKTQRESRALSKSNGREICGLIIDNGHFFELIQ
                     LGNKIKRGGGFAFYFKEVRDVCKMARLCGHIVVGSFHSHPVGLAEPGPSDVQNAMDDS
                     IMLIYDVMGRSAKLWRIKNGKARQRSFSLI"
     gene            complement(2559..>3050)
                     /locus_tag="VG754_10740"
     CDS             complement(2559..>3050)
                     /locus_tag="VG754_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19738.1"
                     /translation="RENCSRLGVTCVEASIAPSGIIANPAKKFDRILVDAPCSNTGVM
                     RRRVDLRWRIRPEEIERLRAGQLDLLRQAAPRLKPGGTLVYSTCSLEPEENAEVMKQF
                     LAEHSQFKMVNERELLPVVDGVDGAYVAKMATSAASLSYPEIQVDLEAETNPKEFIRK
                     QLA"
BASE COUNT          637 a          849 c          812 g          752 t
ORIGIN      
        1 gatcttcatg acgttgggcg gaattgcgcc gcatcttctc ggctattcac acccattgtt
       61 gctgttggaa ttggcgatcg tggtcgcctg gacgctcagc atgtgtttca cctggccgtc
      121 gcttcaagcg ctgctcagcc atgcgcagtc gccgactgaa ttgccgcaca cggcgggcat
      181 ttacaacatg gtttgggcgg gcgcatcggc gctcgcgtat ctgaccagcg gcgcgttgct
      241 ggaccatttc ggcggcgaaa ttttgttttg ggtgcctgcc ggattgcacg tgctgcaact
      301 ggttcttctg cctcgtttgc aaaagatgga tagctcgttg cccgtcgaag aaccagccca
      361 aagcggcgaa tcacttccgg aattgaatcc ccggccaatc gcccgcgcgc ggactttcgt
      421 gaagctggcg tggattgcga atccgttcgc atacatggcc atctacgcgg tcatcccaat
      481 cattccccgg ctggccgcgc agtttaacct gacgccaact tatgcgggcc tggtctgctc
      541 aatatggttt tgggcgcgag tggcggcctt tgccggtctg tggctttggc ccggttggca
      601 ttatcgtttt cgctggttgc tggccgcgtt tctcggattg atcgcgagtt acgtcgccat
      661 cccgctcagc gcaaatcttt ggatgttggt ggccgcgcaa gtggtgttcg gattggtggt
      721 ggggctgatt tattattcgt cgctatttta ttcgatggac gtgggcgaat cgcgcggcaa
      781 acgcggtgga tttcacgaag cggcgattgg catgggcatt ttcgtcggcc cagccgccgg
      841 cgtgggcgcg ttgcaagcgt ttcccggcca gcccaacgcc gggacgtggg ccatcagcgg
      901 attgttggtc atcgggctgg ggctcttttt atggacgcaa tttcgcgaac aacgatgatc
      961 agatatgcga tttacgattt acgcgaggtg gatcatcgga cgcgttcagc cattatgttg
     1021 caaccatcct gcttcccggc aataacgcgt tattgcgata ttttcgttcg attacgactc
     1081 gcccgtaaac gcgtaaatcg taaattgacg ggcacagttt cacttgccat gtttttgaat
     1141 tttcgttctg ctcatcgcga ttaactgaac ttttctcaca tctatgccag aactcagcgg
     1201 aaaactcggt atcgtcttcg gggtcgccaa caaacgctcc atcgcctggg ccatcgcaca
     1261 ggcatggcac aaggccggcg cgcggcttgc gttcacttat caaggcgaac ggctcaagga
     1321 aaatgtcgag gaactcgcag ggactttcgg cgcggacacc ctgctcatgc cctgcgacgt
     1381 gaccaaggac gaagaaatcg cgcgcgtgtt ttccgaggtt ggcgaaaaat tcggcaagct
     1441 gcatctcctg ctgcattccg tggcgttcgc gccgaaggag gcgttggaag gacatttcat
     1501 caacaccagc cgcgaagctt ttcgcatcgc gcatgatgtc agcgcctatt cattgattgc
     1561 gctggcgcgc ggcgccgcgc ccttgatgac cgagggcggg agcattgtcg gcatgacgta
     1621 ttacggttcc gaaaaagtcg tgccgcatta caacgtgatg ggcgttgcca aggcggcact
     1681 cgaagccagc acgcgttatc tggcgtatga tttaggtccg aaaaaaatcc gcgtcaactg
     1741 catcagcgcc ggcccggtga acacattggc tgcgcgcggc attgccggtt tcaccgagat
     1801 gctcaagcat tacgaagccc acgcgccgtt gaaacgaaac gttcttcccg acgaactcgg
     1861 cgcgaccggc acctttctcg ccaccgacgg cgcggcggcc atcaccggcc aagtccttta
     1921 cgtggattgc gggtatcaga tcatggggat gtaagtaact gagagtgaga gccgaacgag
     1981 gtttttcggc ccgaatgcct tcttaggcat tcaaaactca aattaaagaa aatgaccgtt
     2041 gtctcgcctt accattcttg atacgccaca actttgctga acgacccatt acatcataga
     2101 taagcataat cgaatcatcc attgcatttt gcacatccga aggccccggt tcagcaagac
     2161 cgacaggatg agagtggaag cttccaacca caatgtgtcc gcacaggcga gccattttgc
     2221 aaacatctcg aacctctttg aaataaaacg caaatccgcc gcctcgtttt attttatttc
     2281 caagttgtat cagctcaaaa aaatgtccgt tgtcaattat cagaccgcag atctcgcgtc
     2341 catttgattt ggagagtgcc cggctttccc tttgcgtttt tttgaattga gatcgtaaaa
     2401 atttaacgcg atgcatattt cacctgacaa ttccttccta ttaaaaactg ttatcccaat
     2461 tcggagggtt aattgccaac caatcttgcc acatcagcct catctgctca tccgctaaca
     2521 gcagcatgaa cctggtatca gccatggcgc gcatttgtct atgcgagttg tttgcgaatg
     2581 aactctttgg gattggtttc cgcttccagg tcaacttgga tttccggata acttaggctg
     2641 gctgcacttg tcgccatttt cgctacatac gctccatcca ctccatccac aaccggcagc
     2701 aactcgcgct cgttgaccat tttaaattgc gaatgctcgg ctagaaactg tttcatgacc
     2761 tccgcgtttt cctcaggctc cagactgcac gtgctgtaaa ccagcgtgcc gccgggtttt
     2821 aatcgcggcg cggcctggcg gagcaaatca agctgtccgg cgcgcaaccg ctcaatttct
     2881 tcgggacgga ttcgccagcg caaatccaca cgacgccgca tgaccccggt gttcgagcag
     2941 ggcgcgtcca cgaggatgcg gtcgaatttt ttcgccggat tggcaataat gccactcgga
     3001 gcaatggaag cttcgacgca agtcacgcca agccgcgaac aattctcacg
//
LOCUS       DASZMM010000445         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2109797, whole genome shotgun sequence.
ACCESSION   DASZMM010000445 DASZMM010000000
VERSION     DASZMM010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109797"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VG754_10745"
     CDS             <1..540
                     /locus_tag="VG754_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19739.1"
                     /translation="GIIYPQLGVLVGPLAVAAFSDLHADVAIMSAGGITLEGISNSHA
                     LLIDIQRAMLRAAGKVIFCLDHTKFGRRSVAQLCALSEIDIVVTDSDAPTDLIAALHR
                     NGLEIIVAQKTEIPTSPTPASEAELPLAMPKPKAPVAPKPKPAPPLPRRRAPRPIQAK
                     YHPGTESSAESSDSRMGWD"
     gene            complement(551..931)
                     /locus_tag="VG754_10750"
     CDS             complement(551..931)
                     /locus_tag="VG754_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HWF19740.1"
                     /translation="MPTNKKIAVVLVITALLIMIGYWLLPWPKPNPAPPDSDITTMQN
                     QAELGELCKTYHKLTGDWPKNLWKLSSVMMISNTNIIVDGWGHPFAFRLATNPSTTFW
                     LISYGADGLPGGTRSNSDFIMEMH"
     gene            1006..1245
                     /locus_tag="VG754_10755"
     CDS             1006..1245
                     /locus_tag="VG754_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19741.1"
                     /translation="MLTCLMSKLDEIEAAAESLPVEEKQQLLLFLVTRLRAQGAKMLE
                     PRIFSREQIAGWIEEDEADLRRFQQGIKPLAQCVY"
     gene            complement(1217..1876)
                     /locus_tag="VG754_10760"
     CDS             complement(1217..1876)
                     /locus_tag="VG754_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWF19742.1"
                     /translation="MQIAVLGSGMVGDALATKLVQIGQQVMMGSRTANSEGGQKWLKS
                     VGGKAQIGTFADAAAFGEIIISCTNGANALEALRQAGANNLRGKIVIDVSNPLDASKG
                     MPPSLTICNTDSLGEQIQREFPDARVVKALNTVNCYVMVEPKIVPGEHHLPICGNDAK
                     AKREVMAKLCEWFGWKEGSFIDLGDITNARGTEMYLALWIRLWQALGTPKFNIRIVRA
                     A"
     gene            complement(1940..2644)
                     /locus_tag="VG754_10765"
     CDS             complement(1940..2644)
                     /locus_tag="VG754_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF19743.1"
                     /translation="MKTEEKVCVVTGGNRGIGFEICRQLASRGARVVLTARSPEAGQE
                     AVAKLSAQKLSAQFHPLDITASASVEELQKFVESSFGQLDVLINNAGIFLRENGSGLE
                     TDLSVVRKTLETNTLAPLHLSQVLAALLKRSGGGRIVNMSSGLGQLSDMEGGYAAYRI
                     SKTALNAVTRILAAELRGSVAVNSMSPGWVRTDMGGASAERDVSQGADTAVWLALDAP
                     QDLTGQLLHDRKVVPW"
     gene            2752..3075
                     /locus_tag="VG754_10770"
     CDS             2752..3075
                     /locus_tag="VG754_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWF19744.1"
                     /translation="MRPDECSVRAALSVIGGKWKPVIARYLMLGTKRFGELRRAMPDA
                     TQKMLTQQLRELERDGIVERKVFHQVPPKVEYSLTPYGRTLRPVLEALCKWGEKHRAK
                     AARAK"
BASE COUNT          699 a          938 c          776 g          669 t
ORIGIN      
        1 ggaattattt atccgcagtt gggagtgttg gtcggaccgc tcgcggttgc ggcgttttcc
       61 gatttgcacg cggacgtcgc catcatgagc gccggtggca tcacgctcga aggcatcagc
      121 aattcccacg cgctcttgat agacattcaa cgcgcgatgc ttcgcgccgc cggcaaagtc
      181 atcttctgcc tcgaccacac caaattcggc cgccgctccg tcgcgcaact ctgcgcccta
      241 tccgagatag acattgtcgt caccgattcc gatgcgccca ccgaccttat cgctgctttg
      301 caccgtaatg gactggaaat catcgtcgct caaaaaaccg aaattccaac ttcaccaacg
      361 ccagccagcg aagcagaact tcctcttgcg atgcccaagc ccaaagcgcc cgttgcccca
      421 aaaccaaaac ccgcgccacc tcttccgcgc cgccgcgcgc cgcgtcccat ccaggccaaa
      481 tatcatcccg gaacagaatc ttccgccgaa tccagcgaca gccgcatggg ttgggattag
      541 atggcaatga ttagtgcatt tccataatga aatctgagtt cgagcgcgtt ccaccaggga
      601 gtccatcggc tccatagcta atcagccaga aagttgtcga aggatttgtt gccaaccgga
      661 aggcgaacgg gtgtccccat ccgtcaacga ttatgtttgt gttgctgatc atcatcacgg
      721 atgacaactt ccaaagattt ttgggccaat ctcctgtcag tttatggtac gttttgcaaa
      781 gctctcccag ttcagcttga ttctgcatag ttgtgatatc agaatctggc ggagcgggat
      841 tgggttttgg ccatggcaat aaccagtagc ctatcatgat gagtagagca gtaataacca
      901 atacaaccgc aatcttttta ttagtcggca tcggtaataa ttgattgggt tatttatatg
      961 ctcgagtgac cgttcgaacc aattgttttg aatcgctttt aaatcatgct aacgtgcctc
     1021 atgagcaagc tcgatgaaat tgaagcggcg gcagaatcac tgccagtgga ggaaaagcag
     1081 caattgctgc tgttcctggt cactcggctg cgcgcgcaag gcgccaaaat gcttgaaccg
     1141 cgtatatttt cacgcgagca aatcgccggc tggattgagg aagacgaagc ggacctccgc
     1201 cgtttccagc aaggcatcaa gccgctcgca caatgcgtat attaaacttc ggcgtgccga
     1261 gcgcctgcca gagccgtatc cacaacgcga gatacatttc cgtcccgcgc gcgttggtga
     1321 tgtcgccgag gtcgatgaag ctaccctcct tccagccgaa ccattcgcac aatttcgcca
     1381 tgacttcgcg cttggctttc gcgtcgttgc cgcaaatcgg cagatgatgc tcgccgggca
     1441 caatcttcgg ctcgaccatc acgtaacaat tcacggtgtt caacgccttg accacccggg
     1501 cgtcggggaa ctcgcgctgg atctgctctc caagggaatc ggtgttgcat atggtgagcg
     1561 acggcggcat tcccttcgat gcatcaagcg gattggaaac atcgatgaca atcttgccgc
     1621 gcaaattatt cgcgcccgcc tgacgcaacg cctccaacgc attcgcgccg ttcgtgcaac
     1681 tgatgatgat ttcaccaaac gccgctgcgt ccgcgaacgt gcctatttgc gccttgccgc
     1741 cgaccgattt cagccatttc tggccgcctt cgctgttcgc ggtgcgcgaa cccatcatca
     1801 cctgctggcc gatttgaaca agtttcgtgg cgagcgcatc gcccaccatg ccggaaccga
     1861 ggactgcgat ttgcatagat gtagttttct tgttgttttt ggttgtgact gaatttctgc
     1921 aaaaaatctc gcgacggcgt caccagggaa cgaccttgcg atcgtgcagc aattggccgg
     1981 taaggtcttg cggcgcgtcc aacgcgagcc ataccgctgt gtccgcgcct tgcgaaacgt
     2041 cccgttcggc actcgcgccg cccatatccg tcctgaccca gccgggagac atcgaattga
     2101 ccgcgacact tccgcgcaat tcagccgcga gaatgcgcgt gaccgcgtta agcgcagtct
     2161 tggaaatccg atacgccgcg tagccgcctt ccatgtctga aagctgcccc aacccgctcg
     2221 acatgttcac gatgcgacct ccgccactgc gcttgagcaa cgccgcgagc acttgcgaca
     2281 gatgaagcgg cgcaagcgtg ttcgtctcca acgtctttct caccacggaa agatcagttt
     2341 cgaggccgga accattctct ctcaaaaaaa tgcccgcatt atttatcaac acatccaatt
     2401 ggccgaaact gctttcaaca aatttttgca gttcttcaac gctcgccgac gcggtgatat
     2461 ccagcggatg aaactgcgct gacaattttt gcgcggaaag cttcgccaca gcctcctggc
     2521 ccgcctcggg agaacgcgcc gtcaggacga cccgcgcgcc gcggctggca agttgacggc
     2581 agatttcaaa accgatgccg cgattgccac cggtcacgac acagactttt tcttcagttt
     2641 tcataaaatg ttgaatgcgc ttgccagtcc aggacaacgc cgccaatgta gcccacagag
     2701 agaaagcggc aagtaggcac aaatttgtgc catagacact ttttggaaac catgcgcccc
     2761 gacgaatgct ctgtccgcgc cgccctgagc gtgatcggcg gaaaatggaa accggtcatc
     2821 gcccgttacc tgatgctcgg cacgaaacgt ttcggcgaac tgcgccgcgc gatgccggat
     2881 gccacgcaaa aaatgctcac ccaacaactg cgcgaactgg agcgcgacgg catcgtcgaa
     2941 cgcaaagtgt ttcaccaagt cccgcccaaa gtggaatatt ccctcacgcc ttatggccgc
     3001 acgctgcgtc cagtcctcga agccttatgc aaatggggcg agaaacaccg cgccaaagcc
     3061 gcccgcgcca aataacccga cg
//
LOCUS       DASZMM010000446         7826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4860796, whole genome shotgun sequence.
ACCESSION   DASZMM010000446 DASZMM010000000
VERSION     DASZMM010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4860796"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VG754_10775"
     CDS             complement(<1..215)
                     /locus_tag="VG754_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HWF19745.1"
                     /translation="MAWLTDRHYFLLAVIIYGVSTIYSIFLWRKGFRQDNRNNYFLLF
                     AAFALHTTAMVKRGFSLERCPVNNLYEA"
     gene            complement(363..1739)
                     /locus_tag="VG754_10780"
     CDS             complement(363..1739)
                     /locus_tag="VG754_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWF19746.1"
                     /translation="MSARKIMLLLLAFGLLVGVLPLRAQTNTLVWNKSQDTMDADVTS
                     WDVLQLLKHVAGSTGWKVYLDPGAIHGVSAKFKGKPTGDALHTLLGNLNFMLVPEANG
                     PSRLYVFRVNRQQATRLITAPKKVAKPIPNQLVVTLKPGSKTKIEDLARALNAKIIGR
                     MDAENAYLLQFQDDADTQFALQQLASNADVASIDNNFPMEAPPVFTAADGSNMPDLQL
                     NPATNNNCQLVVGLIDTPVQSLGNNLNAFMQPAIHVAGNVSIPANQLTHGTAMAETIM
                     DSLQSKTGGSTTVKILPVDVYGNNETTSTFDVANGLIQAVNNGADIINMSLGGTGDSQ
                     ILHDAINQVLNRGIPIYAAAGNEPVTTPTYPAAYPGVIAVTATDTTGQIASYANRGSF
                     IDMAAPGDNVVGFDGQSYTVEGTSTSTAFISGAAAGLADVNHQCADQAQSLLQKTLPK
                     SSLLKPSQ"
     gene            complement(1762..4353)
                     /locus_tag="VG754_10785"
     CDS             complement(1762..4353)
                     /locus_tag="VG754_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HWF19747.1"
                     /translation="MLRTLILGLCFMAVFTFNVHAGGSGLNVAIIVNQNSTNSVQLGN
                     YYRAQRNIPPQNYFRINWTGTNTEWTGTDFTNTLLNPFLSMLSSRQLTNQIDYVVLSM
                     DIPYRVTSGSSEPNSTTSSLFYGFKTDNNPPCSMANNSSNAYAGSECIFRQTPALSGG
                     SNFFMVTMLISSNDLSLGEMEVSQGVLSDGTFPTNTVYLCTTMQPGYDARYPEFDNAI
                     FNTRLRGDYSMVRSNIGAFGFPPYVLGSQQGVYAVGLPNPPYFSPGAMVDDLNSFGGN
                     FLEDTSGETTVVKYFYLGACGSYGTVTEPCAYLEKFPDSQNYFYQARGFSVGECYYQS
                     VTNIYQGMVCGEPLAAPFAQKGTGAWNGLASNAILSGITNLSGLFTASDAKHPLQQVD
                     LFLDGNWLQTATNIPPQSGNLLKVSLNGFATSYTVPANATIKSVTSNLVNTLNNSVYQ
                     SSTKVFAIDHGDRIELQSTASYTKTGAQISIMVSSTNSSGALTTFIRTADAAPTNFLD
                     STAQGIRYYDLGGTLSSNSTLTLTVTKTNNSVVTVTMTNNGNTTFTGFAQQMVTAINN
                     TASLQGLDGLYGEDVETGNVGDALFNLQPQAQGYAAAQIKAGFTASSGIVLAPSGTST
                     LTDNTTDLEPRQHLYITAGVSNLAFVTPFVTTNFPDGYHELIAVAYEGSHVHTQTRAT
                     QEIIIKNSGLSATLKPVLTGSNSTLQPTLQIIVTANTNNISSIQLFSTGGLLASATNQ
                     NSATFPIVLTNLDVGVHPFYAIVTQTNGNQYRTQTQNIGLIGVTYSSTTLMGVDYPFP
                     LQFTAPPAMLVWPATAGRSYTVLSTTNLTMPFQTRATVLPTNSLGQWAETNSAPAQQF
                     YRVSVSP"
     gene            complement(4402..5724)
                     /locus_tag="VG754_10790"
     CDS             complement(4402..5724)
                     /locus_tag="VG754_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWF19748.1"
                     /translation="MSAPAQNKIKSAATMLALAGVLVAILGMAYLFLTPKSYRATARI
                     KVEKSNWINHQAIRDAQNSSDSHLASAECQVLRSNAFLDRVVQNLDLSHEWGKRYRQG
                     TPLSQDDAREMLRSKSDIHSMPDSSIIVISVTSEESEETAKIANELARLYTNYCQSQR
                     QDTIHGKVDSLQHQWEVQNKKVQDTQLKLQQIKLEIKRDRVRATNGPIFYDSDNYGLL
                     QNKRIELESQTVQLRDQLNELKMLQPAEQRQVLSSLDTETNSVLNTSLAQLRKAKTDL
                     ADAESDHGADSPEVKHAQKIVDEMEKSLTRTVSGIIMMKQADLAADEAAFKALDDELK
                     NIKAPNQVDTNILQNPAFAQTYQELQQLEHDRDALQDQMNSTYTKEAFQPVVITAELT
                     DSAETPSRPFMPDSRVALGGIGGGGALSLAGLGLLLLIQMRKSALRKS"
     gene            5907..6884
                     /locus_tag="VG754_10795"
     CDS             5907..6884
                     /locus_tag="VG754_10795"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF19749.1"
                     /translation="MNEPLLSVTVLIACRPGQAEIKAVNAARLLDYPREKLEIIVARG
                     KQPSVQRNTAMRAARGELIYFLDDDSTPPPGNLRDAVASFADPHVKMLGGPNLCPPDA
                     PFLEQVFALVHASWLAFSSSRARYASVGKVRESSEKELILCNLIARRDAMLDLGGFNE
                     ALYPNEENALMDELQKGGGKLLYDPALIVCRRPRHSLKAFAKMLLNYGRGRAEQFRLH
                     PTPGSAMNFVPPMFCLYLVVLAGLAALGRNSLLAWSAIPLLFYFIAVFLQAVVLIPSG
                     GIVRSVCAIPLIALTHILYGVGFWRGLFTTLKPSENRPKTTVELENVPL"
     gene            6898..>7826
                     /locus_tag="VG754_10800"
     CDS             6898..>7826
                     /locus_tag="VG754_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-ammonia-ligase adenylyltransferase"
                     /protein_id="HWF19750.1"
                     /translation="MKNPVWSKALKASADPQRARHFLDLLASTNARSPLERASPEQAR
                     ILCALFSGSQALSNWLVVHPEALALLLPEALAHPRRSQGFRRETDEWLSPLLQSGDYS
                     GALARLRQFKQREMLRIAARDLARLGDVTEIIREVSDVADVCLDSVWQICHRQISERL
                     GKPFHKDAQGRWQPTEFCVFGLGKLGGRELNYSSDVDVIFVYSEEGQVFKQQPAKKAH
                     DIGGVGNHQFFNRLAEQFIGEVGRMSGEGMLYRIDLRLRPEGNSGPLARSLSSYENYY
                     AQWGQTWERMMLLKARRVAGNEALANEFLEMIHP"
BASE COUNT         1734 a         1921 c         2215 g         1956 t
ORIGIN      
        1 gcctcgtaaa ggttgttgac agggcagcgt tcgagtgaga aaccgcgctt gaccatggct
       61 gtggtgtgca aagcgaaggc ggcgaacagc aggaaataat tattgcgatt gtcctgacgg
      121 aagcctttgc gccatagaaa gatggaataa atagtgctca ccccgtaaat tatcacggcg
      181 agtaggaaat aatgtctgtc cgtcaaccaa gccacgcgct tagattaaac cagagcggca
      241 agcctgcaag cggcaaaatg gcgcggggac acgccgttac aaggttaaaa ggttacaaag
      301 ttacatcagc atcgctcgta ctttgagcaa cacctttgta atgatgtgac attttaacga
      361 tcctattgcg atggcttcaa cagggaagat ttggggaggg ttttctggag cagagattgc
      421 gcctggtcgg cacattgatg atttacatca gccaaaccag ccgcggctcc gcttataaaa
      481 gccgtcgagg tggaagtgcc ttcaaccgta taactttgcc cgtcaaaacc gaccacgttg
      541 tctcccggcg cggccatgtc aataaaactg ccacggttcg cgtaagaagc gatttgcccg
      601 gtggtgtccg ttgcggtgac ggcaatcaca cccggataag cagccggata ggtgggcgtc
      661 gtgaccggct cattgccagc ggcggcgtaa atcggaatgc ctcgattgag tacctgattg
      721 attgcatcgt gcaatatctg gctgtcaccc gtgcctccca agctcatgtt gatgatgtcc
      781 gcgccattgt tcactgcctg aatgagacca ttggcgacat cgaatgtgct ggtggtttca
      841 ttgttgccgt aaacatccac aggaagaatt ttcaccgtgg tgctgccgcc ggttttcgat
      901 tgcaaggaat ccatgatggt ttcagccatg gccgtgccgt gagtcaattg attcgcagga
      961 atgctcacat tgcctgcgac gtgaatcgcc ggttgcataa aggcattcaa gttgttgccc
     1021 agtgattgca ccggagtgtc aatcaatcca acgacgagct ggcaattgtt gttggtcgcg
     1081 ggattgagtt gtaaatccgg catattgctg ccgtccgcag cggtgaacac tggcggcgct
     1141 tccatgggaa aattattgtc aatgctggcg acatccgcgt tgctcgccaa ctgctgcagg
     1201 gcaaactgag tgtcggcatc atcttgaaat tggagaagat aggcgttctc ggcatccatg
     1261 cggccgatga ttttggcgtt cagcgcgcgg gcgaggtctt caattttggt cttcgagccg
     1321 ggtttcaagg tgaccacgag ttgattggga attggtttgg caactttctt cggcgcagtg
     1381 atgaggcgtg tcgcttgctg gcggttgacg cggaatacat aaagacgcga aggaccgttg
     1441 gcttccggaa caagcatgaa attcaaattg cccagcagcg tgtgcagcgc gtcgcccgtc
     1501 ggtttgcctt tgaatttggc ggaaacgcca tggattgcgc ccggatccag ataaaccttc
     1561 cagccggtcg agccggccac atgcttcaag agttgcagaa catcccatga cgtgacgtcg
     1621 gcatccatcg tgtcctgaga tttattccat acgagcgtgt tggtttgggc tcgcaacggc
     1681 aggacgccga cgagcaggcc gaaagccaac agcaggagca ttattttgcg agcggacata
     1741 tatcgattgc cacttgtata actaaggtga aacactgacg cgataaaatt gttgcgcggg
     1801 cgcactattg gtctcagccc attggccgag ggaattggtc ggaaggaccg tggcgcgggt
     1861 ctggaatggc atcgtcaaat tcgtggtgct caagaccgtg taactgcgtc cggccgtggc
     1921 aggccagacg agcatcgcgg gcggagcggt gaattgcaaa ggaaatggat aatccacacc
     1981 catcaacgtt gtgctggagt atgtcacacc gatcaacccg atattttgcg tctgagtgcg
     2041 gtattgattg ccgttggttt gcgtaacgat ggcgtaaaat ggatgcacac cgacgtccag
     2101 gttcgtcagc acaatgggga acgtggctga attctggttc gtagccgacg ccagcaaacc
     2161 gccggtgctg aataattgaa tcgaactgat gttgttggta ttggcggtga cgatgatttg
     2221 cagggttggc tgcaaagtgg aattcgagcc ggtgaggacc ggcttgagcg ttgctgaaag
     2281 cccggagttt ttgatgatga tttcctgcgt ggcccgggtt tgcgtatgaa catgactgcc
     2341 ttcatacgcg accgctatca attcatggta accatcgggg aaattggtgg tgacaaacgg
     2401 agtgacgaaa gcaagattgg aaactccggc cgtaatataa agatgctgcc gcggctcaag
     2461 gtcggtggtg ttgtcggtga gcgtgcttgt gcccgatggt gcgaggacaa tgccggaaga
     2521 tgcagtaaaa cccgccttga tttgcgcggc ggcatatcct tgggcttgcg gctggagatt
     2581 gaacaaggcg tctccaacat ttccagtttc cacgtcctcg ccatacaggc catccaatcc
     2641 ttgcaggctt gcagtattgt tgatggccgt gaccatttgc tgggcaaacc cggtgaaggt
     2701 ggtattgccg ttgtttgtca tggtcacggt cacgacgctg ttgttcgttt tggtgaccgt
     2761 caaagtaagc gtgctattgg aggacaaagt tccacctaga tcgtaatacc gaatgccttg
     2821 cgctgttgaa tcgagaaaat tcgtgggtgc tgcatcggca gtacgaataa atgttgtgag
     2881 cgctccggag gaattggtgc tgctgaccat gatggaaatc tgcgcgccgg tcttggtata
     2941 tgaagccgtg gattgcaatt caatcctgtc tccgtgatcg atggcgaaga ccttggtgct
     3001 gctttgatag acggagttgt tcaacgtgtt caccaggttg ctggtgacgg atttgatggt
     3061 tgcgtttgct ggaacagtgt aactcgtggc gaacccattc aggctgactt tcaaaagatt
     3121 gccggattgt ggcggaatgt tggtcgcggt ctggagccaa ttgccatcca gaaacaagtc
     3181 cacctgttga agcggatgtt tggcatcgct ggcggtgaac aggccggaaa gattggtgat
     3241 cccggaaagg atcgcattgc tcgcaagtcc attccatgcg cccgttccct tttgggcaaa
     3301 tggcgcggcc agaggttctc cacataccat tccctgataa atattcgtga cgctctggta
     3361 atagcattct cccacactga acccgcgcgc ctgataaaag taattctggg agtcgggaaa
     3421 tttttcaaga taagcgcatg gttcagtgac ggtgccgtaa cttccacagg cacccagata
     3481 aaaatacttt accactgtgg tttcccctga agtatcttcc agaaaatttc cgccaaatga
     3541 attgaggtca tctaccatgg ctccaggaga gaagtatggc ggattcggca ggccgaccgc
     3601 atacacgcct tgttgggagc cgagaacgta aggaggaaag ccgaatgccc cgatgttgct
     3661 tcgcaccatg gaataatcgc cgcgtaaccg cgtgttgaat atggcgttgt cgaattctgg
     3721 atagcgcgcg tcatagcccg gctgcatggt tgtgcaaaga taaaccgtat tcgtagggaa
     3781 agtgccgtcg cttaaaacgc cctggctgac ttccatttct ccaagggaaa gatcattgct
     3841 ggaaatcaac atcgtcacca tgaagaaatt gctgccgccg ctgagggccg gagtttgtcg
     3901 aaaaatacat tcgctgcctg cgtaggcgtt cgacgaattg ttggccatgg aacaaggagg
     3961 gttgttgtcc gttttgaatc cgtaaaataa agaagaggtc gtgctgttcg gttcgctgct
     4021 gccgctggtc acgcggtaag gaatgtccat cgagagcacg acgtaatcaa tctggttcgt
     4081 caactggcgg ctggacagca tcgaaagaaa cgggttcagc agcgtgttcg tgaaatcggt
     4141 acccgtccat tcggtgttgg tgccagtcca gttgatgcgg aaataatttt gcggaggaat
     4201 gttgcgttgc gcgcggtagt aattgcccaa ctgcacggaa ttcgtgctgt tctggttgac
     4261 gatgattgcg acattaagcc cgcttccgcc cgcatggacg ttgaatgtga atacagccat
     4321 gaagcagagg ccgagaatca gcgttctgag catttctcaa taatcgccga aattgtaaaa
     4381 tagtaaagcg cgcggaagcc atcaactttt tcgcagcgca gacttccgca tctgaatcag
     4441 caataataac cccaagcctg ccaatgacaa tgcgcctcca ccgccgatgc cgccaagcgc
     4501 gacgcggctg tccggcatga aagggcgcga tggagtttcg gctgagtcgg tgagctcggc
     4561 agtaatcact acaggctgga acgcttcttt cgtgtaggtt gaattcatct ggtcctgcaa
     4621 agcatcacgg tcgtgttcga gttgttgcaa ttcttgatac gtctgtgcga aggcgggatt
     4681 ttgaagaatg ttcgtgtcca cttgatttgg ggcttttata tttttcaatt catcgtccaa
     4741 tgccttaaac gccgcttcgt ccgccgcaag gtcggcttgt ttcatcatta ttattccgct
     4801 gacggttcgg gtcagcgact tttccatctc gtccacaatt ttttgcgcgt gcttgacctc
     4861 cggcgaatcc gcgccgtggt ctgattccgc gtcggccaga tcagttttgg ctttccgcag
     4921 ttgagccaac gaggtgttca aaactgaatt ggtttctgtg tcgagcgagg acaagacctg
     4981 gcgttgttca gcgggttgca acattttcaa ctcgttcaac tggtcgcgca attgaacggt
     5041 ttgggattcc aattcgatgc gcttgttctg caacagaccg tagttgtctg aatcgtagaa
     5101 aattgggccg tttgtggccc ggactcggtc ccgcttgatc tccaatttga tctgctggag
     5161 tttcaattga gtatcctgga cctttttatt ttgcacctcc cactgatgct ggagcgaatc
     5221 aaccttgccg tgaatggtgt cctgacgttg tgactgacag taattggtgt aaagtcgcgc
     5281 aagctcattg gcgattttgg cagtctcttc agactcctcg ctggtcacgc ttatcacgat
     5341 gatgcttgag tccggcatgg aatgaatgtc cgacttcgaa cgcagcatct cacgcgcgtc
     5401 atcctgagat aacggagttc cctggcgata ccgttttccc cattcgtgtg acaaatcaag
     5461 attttgcaca acacgatcga gaaaggcgtt ggaacgaagc acctggcatt cggcggaagc
     5521 caaatgagag tcagaagagt tctgggcatc tctgatggct tggtgattga tccaattact
     5581 tttttcaacc ttgatcctgg cggtcgcccg gtatgacttc ggcgtcaaga aaaggtaagc
     5641 cattcccaaa atggcaacga ggacaccagc cagcgcaagc attgtcgcag cggatttgat
     5701 tttattttgc gcaggagcgc tcatgatttt ggaagacgca ctttgagcat gagattacga
     5761 ttaagcaatc gcagtgtgat tattttggag cgcatctttg gattttactt tgtttggaca
     5821 gttactctgc cgtttcgttc atcacctcta tcaggaagaa actttgacct ttcaacttgg
     5881 aactttgaac ggctagctta tgctgcgtga atgagccatt gctgagtgtg actgtgttga
     5941 tcgcctgccg acctggccag gctgagatca aggctgtcaa tgccgcccgt ctgctcgatt
     6001 atccgcgcga aaagctcgaa attattgtcg cgcgcggcaa acagccgtcc gtccaacgca
     6061 acaccgccat gcgcgcggcc cggggcgaat tgatttattt tctcgatgat gattcaacac
     6121 ctcctccggg caatctgcgt gatgcggtcg catcgttcgc tgacccgcat gtgaagatgc
     6181 taggcggccc aaatctttgc ccgcccgacg cgccgttttt ggagcaagtc tttgcgctcg
     6241 ttcacgcaag ctggctggca ttctcttcca gccgcgcccg ttatgcgtca gtcggcaaag
     6301 tgcgcgaatc gagcgaaaag gaattgattc tttgcaatct catcgcccga agagacgcga
     6361 tgcttgatct gggtggcttt aatgaagctt tgtatccaaa tgaggaaaac gcgttgatgg
     6421 acgaattgca aaagggcggc ggcaagctgc tttacgatcc ggcgctgatc gtttgtcggc
     6481 gacccaggca cagtttaaag gcgttcgcaa aaatgttgct caattacgga cggggcaggg
     6541 cggagcagtt ccggctgcat ccgacacccg gttctgcgat gaattttgtt cctccgatgt
     6601 tttgcctcta tctggtcgtg cttgctggat tagctgcttt gggtagaaac agcttgctgg
     6661 catggtcggc cattccgctg ctgttttact tcatcgctgt gttccttcaa gccgttgtcc
     6721 tgatcccaag cggcggcatc gtgcggagcg tttgtgccat tccactgatt gctctcacac
     6781 atatactata tggcgtcggt ttttggcgtg gattgttcac cactttgaaa ccatccgaaa
     6841 atcggcccaa aaccaccgtg gaattggaga acgtcccgtt gtagattgcg tttctgaatg
     6901 aagaacccgg tctggtcgaa ggcgttgaaa gccagcgcgg acccgcaacg cgcccggcac
     6961 tttcttgatt tgctggcgtc cacgaacgcg aggtcccctc tggaacgcgc ctctcccgaa
     7021 caggcgcgca tcctctgcgc gttgttcagc ggctctcaag cattgagcaa ctggctcgtt
     7081 gtgcatcccg aagcgctcgc gttgctcctg cccgaagcgc ttgcgcaccc gcgacgctcg
     7141 caaggcttcc ggcgcgaaac ggacgaatgg ctttcgccgc tcttgcaatc cggcgattac
     7201 tcgggcgcat tggcgcgtct gcgtcagttt aaacaacgtg aaatgttgcg catcgccgcc
     7261 cgcgatctcg cgcggctggg cgacgtcaca gaaatcatcc gcgaagtgtc cgatgtcgcc
     7321 gatgtctgcc tggattccgt ctggcaaatc tgtcatcgcc agatcagcga acgtctcggc
     7381 aagcctttcc ataaagatgc ccagggccgc tggcaaccga ctgagttttg tgtgttcggc
     7441 cttggcaaat tgggtggacg ggaattgaat tacagttcgg acgtggatgt cattttcgtt
     7501 tacagcgagg aagggcaggt ttttaaacaa cagccggcaa aaaaagcgca cgacatcggt
     7561 ggtgtcggca atcatcaatt tttcaaccgg cttgcagagc aatttattgg cgaggttggt
     7621 cgcatgtctg gcgaagggat gctttatcgg attgatttgc gattgcggcc tgaaggcaac
     7681 tcgggcccgc tggcgcggtc actctcgagc tatgaaaatt attacgcgca atggggccag
     7741 acttgggagc gcatgatgct cctgaaggcg cggcgcgtgg caggcaatga agcgctggca
     7801 aacgaatttt tggagatgat ccatcc
//
LOCUS       DASZMM010000447         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1320176, whole genome shotgun sequence.
ACCESSION   DASZMM010000447 DASZMM010000000
VERSION     DASZMM010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320176"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="VG754_10805"
     CDS             complement(<1..1562)
                     /locus_tag="VG754_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006630922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HWF19751.1"
                     /translation="MQSDSQKQPNQNRKPNGLPPKQGLYDPQFEHDACGVGFVVNVKG
                     KKSHEIITQALTILLNLDHRGACGCEVNTGDGAGILMQMPHAFLQKVCAANGIELPAE
                     GHYGAGIIYLPPDANERKECEQIFGKIVAEEGQHIIGWRDVPTRNSTLGETAKSSEPF
                     MRQVFIKRNAKLTDDMAFERKLYVIRKRATNEIRRAGFPGSEYWYVSSLSHKTLVYKG
                     MLNTVQVAEYFADLADPTMTTALALVHSRFSTNTFPSWERGHPYRYIAHNGEINTLRG
                     NINWMHARQAMFESELLGPDLKRILPIINTNGSDSAMFDNCLELLVMAGRSLPHAMMM
                     MIPEPWTNHESMSDEKKAFYEYHSCLMEPWDGPASIAFTDGKQIGAVLDRNGLRPSRY
                     YVTKDDLVIMASEVGVLDIPPDRILQKGRLQPGRMFLVDTVEGRIVADEEIKEKIASA
                     HPYREWLDKYMVELGSVDDAPHGPEPSHETVLRRQQAFGYTFEDLRILMAPMAATGVE
                     AVGSMGTDTPLAV"
     gene            complement(1854..2516)
                     /locus_tag="VG754_10810"
     CDS             complement(1854..2516)
                     /locus_tag="VG754_10810"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HWF19752.1"
                     /translation="MKIFAIIFLTVTLSAKWAAADEIIFQDEFKGHLGNGWSWVREHR
                     DAWRVSGPAPGLEVLIEPGNMWGPANNAHNLLVRAAPDASSNEIQISVTFETHPTNQY
                     EQADLTWYFDDSNMVKIGRELVDGKVSVVMGREEKDKTRTIAIIPIKTDTVRLQLEVK
                     GNHIRGRYLSPDGTKWLQAGECDLPPSNLKPRISLQFYQGPANSTHWARVTGFRVIRL
                     GN"
     gene            complement(2531..4012)
                     /locus_tag="VG754_10815"
     CDS             complement(2531..4012)
                     /locus_tag="VG754_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWF19753.1"
                     /translation="MTHPLLYEINTRCWLRQLSEQQGKAVTLGNVPDEEIENWRKLGF
                     THVWAMGIWTTGPLCRAQALENPHLQERFTKILPGWSKHDVTGSPYAIGSYEVPAFLG
                     GANGLKIFRQQLHAHGMKLLLDFVPNHLGLDHPWVSEKPEMFVQSPTELPGTFSQPTR
                     NGPRWLAHGKDPNFPPWSDTVQLDYRCSETRAAMIELLQSVSAQCDGVRCDMAMLLLN
                     EVFKKNWAHFPEVEDFPASEFWTDAIAAVRQSRPDFIFLAEVYWGLEANLQELGFDYT
                     YAKTVYDRLVDGHSAALQKELLAAKSGWLARSAHFLENHDERRAASVFSNDRHRAAAL
                     LMLGLPGMRMIYEGQLRGARIQVPVHLARWPNEPIDEKISAMYGQLLSVLKDSAVGRG
                     DFRILQPENLGDLSVAQNFIVIQWQKTRELFEWVVVNLANEHGQCRLQPAIAELAGHN
                     WEMRDLLGTEIKNFHGGDLEGQGIHLALPAYGAQLLRFARTPA"
BASE COUNT          898 a         1161 c         1101 g          961 t
ORIGIN      
        1 accgcgagcg gcgtgtccgt gcccatggaa ccgactgctt caactcccgt tgcggccatc
       61 ggcgccatca agatgcgcaa gtcttcaaac gtgtaaccaa acgcctgctg acggcgcagc
      121 acggtttcat ggctcggttc agggccgtgt ggcgcgtcat ccacgctgcc gagttcgacc
      181 atgtatttat cgagccattc gcggtaagga tgcgcggagg cgatcttctc cttgatttcc
      241 tcgtcggcaa cgatgcgccc ttcgacggtg tccaccaaaa acatgcggcc cggttgcagg
      301 cggccttttt gtaaaattct gtcgggcgga atgtccagca cgccgacttc cgaagccatg
      361 atgaccaaat catccttcgt cacgtaataa cgcgaaggcc gcaatccatt ccggtcgagc
      421 accgcgccga tctgcttgcc gtccgtgaat gcgatggaag cgggaccgtc ccacggttcc
      481 atgaggcagc tatgatattc atagaacgcc ttcttttcgt cgctcatgct ttcgtggttg
      541 gtccacggtt cgggaatcat catcatcatc gcgtgcggca atgagcgccc cgccatgaca
      601 agcaactcaa ggcaattgtc gaacatcgcc gaatcgctgc cgttggtgtt gatgatgggc
      661 agaatgcgct tgaggtccgg ccccaacaat tcggactcga acatcgcctg gcgcgcgtgc
      721 atccagttga tgttgccgcg gagcgtgttg atttcgccgt tgtgcgcgat gtaacgatac
      781 ggatggccgc gttcccaact tggaaaagtg ttcgtgctga aacgcgaatg caccaacgcc
      841 agcgcggtcg tcatcgtcgg gtctgcaagg tcggcaaaat attcggcgac ctgcaccgtg
      901 ttgagcattc ctttatagac gagcgtcttg tgggaaaggc tcgacacata ccagtattcg
      961 ctgcccggaa aaccggcgcg gcggatttcg ttggtggcgc gtttgcggat gacgtagagc
     1021 ttgcgctcga acgccatgtc gtcggtcagc ttggcattgc gcttgatgaa aacctggcgc
     1081 atgaacggct cggaagactt ggccgtctcg cccaacgtgg aatttctcgt gggaacatca
     1141 cgccagccga tgatgtgctg gccttcctcg gcgacaatct tgccaaaaat ctgttcgcat
     1201 tccttgcgct cgttcgcgtc cggcggaaga taaataatcc ccgcgccgta atggccttcg
     1261 gcaggaagtt cgattccgtt ggcggcgcaa actttttgca aaaaagcgtg cggcatctgc
     1321 atgagaatgc ccgcgccgtc gccggtgttg acctcgcatc cgcaagcgcc gcgatggtca
     1381 aggttcagca ggatggtcaa tgcttgggtg atgatctcgt gggatttctt gcccttgacg
     1441 ttgacgacaa agccgacgcc gcaggcatca tgctcgaatt gcggatcata caaaccctgt
     1501 ttgggaggca gtccgttcgg tttccgattc tgatttggtt gcttctgact gtctgattgc
     1561 atcgtttatg gccggcaaac ccatgccggg aataaaattt tgttattttt tgcagaaatt
     1621 tccgcagaac gcaatagcta gattcaaata attttgtggc tctttcacat ttttacgata
     1681 cctatccaca ttttctccgt cgcctccatt ggcaagaggc tggggtgagg gggcaaagcc
     1741 gagtcacgtc aataacaagc ccatcggaac tggcgcaaac agccgcagcc tcgcttccaa
     1801 agaatcttct ccattttggc atttttgcca gaggagtttt gagtgccaga gggtcagttt
     1861 ccgagccgta tgacgcggaa tccggtgacg cgggcccaat gtgtcgaatt ggctggacct
     1921 tgatagaatt gcaggctgat gcggggtttt agattggatg gcggcaggtc gcattcaccg
     1981 gcttgcagcc actttgtgcc gtccggggat aaataccgac cgcgtatgtg gttgcctttc
     2041 acttcaagtt gcaggcgaac ggtatcggtc ttgatgggga tgatggctat tgtgcgcgtc
     2101 ttatcttttt cctcgcggcc catgacgacg ctcactttgc cgtccaccaa ttcgcgccca
     2161 atcttcacca tgttgctgtc gtcaaaatac caggtcaaat ccgcctgctc gtattggttg
     2221 gtcgggtgcg tttcgaatgt gacagaaatt tggatttcgt tgctcgaagc atccggagcg
     2281 gcgcggacga gaagattatg cgcgttgttt gccgggcccc acatattgcc cggttcaatg
     2341 agcacttcaa gtccgggcgc gggaccggac acccgccacg catcgcgatg ctcgcgcacc
     2401 caagaccatc cattgccgag atgacctttg aattcgtcct gaaaaatgat ttcatccgct
     2461 gcagcccatt ttgcggacaa cgtcactgtc aggaatatga ttgcgaaaat tttcaccgct
     2521 atttttcact ttacgccgga gtgcgggcaa accgcagcaa ctgcgcgcca tacgccggaa
     2581 gcgccagatg aatcccctgc ccttccaaat caccgccatg aaaatttttg atctcagttc
     2641 ccagcaagtc gcgcatttcc caattgtgcc cggcaagttc cgcaattgcg ggttgaaggc
     2701 ggcattggcc atgttcattg gccaagttca caacgaccca ttcaaatagc tcccgtgttt
     2761 tttgccattg gatgacaata aaattttgcg ccacggaaag gtcgcctaaa ttttctggct
     2821 gaaggatgcg aaaatcaccg cgaccaactg ccgaatcctt gagcaccgaa agaagctgtc
     2881 cgtacattgc agaaattttt tcgtcaatcg gttcattcgg ccagcgggca agatgtaccg
     2941 gaacttgaat ccgcgcgccg cgaagctgcc cttcgtaaat catccgcatt cccggcaagc
     3001 caagcatcaa caacgccgcg gcgcgatggc ggtcgttgga aaaaacggac gcggcgcggc
     3061 gttcgtcgtg gttctccagg aaatgcgcgc tgcgcgcgag ccacccggat ttcgccgcca
     3121 acaattcctt ttgcaacgcg gcggaatgtc catccaccaa acgatcgtaa actgtcttgg
     3181 cgtaagtgta atcgaaacca agctcctgca agttcgcctc caagccccaa tacacttcgg
     3241 ccaaaaaaat aaaatctggc cgcgattgcc tgaccgccgc gattgcatct gtccagaatt
     3301 cactcgcggg gaaatcttca acttcgggaa aatgcgccca attttttttg aaaacttcat
     3361 tgagcaacag catcgccatg tcgcatcgca cgccatcaca ttgcgcggag acggattgca
     3421 acaattcaat catcgcggcg cgggtttccg agcagcgata atcaagctgc acggtatcgg
     3481 accaaggcgg gaagttcggg tctttgccat gcgccagcca gcgcgggccg ttgcgcgtgg
     3541 gttgcgaaaa tgttcccggc aattccgttg ggctttgcac aaacatctcg ggtttttcac
     3601 tgacccacgg atggtccaac ccaagatgat tcggcacgaa atccaagagc aacttcatcc
     3661 catgcgcatg aagttgttgg cggaaaattt tcaggccgtt tgctccaccc aaaaaggccg
     3721 ggacttcgta gctcccgatg gcataaggcg aacccgtgac atcgtgcttg ctccagccgg
     3781 gaagaatttt tgtgaatctc tcctgaagat gcggattttc gagcgcttga gcgcggcaca
     3841 acggcccggt cgtccagatg cccattgccc agacatgcgt gaaaccgagt ttccgccagt
     3901 tttcaatctc ctcgtccggg acattcccca gcgtcaccgc cttgccctgt tgctcggaaa
     3961 gctgccgcaa ccagcaacgg gtgttgattt cgtaaagcaa cggatgagtc atgcgtcaag
     4021 gctttgcaat ggcttgcaaa aacttggcat ctacagccgc ttcacgcaag cagaagcaac
     4081 gcgctcaacg cacgactcgc acccaactgt gccggcgaaa a
//
LOCUS       DASZMM010000448         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4861243, whole genome shotgun sequence.
ACCESSION   DASZMM010000448 DASZMM010000000
VERSION     DASZMM010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4861243"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /gene="tilS"
                     /locus_tag="VG754_10820"
     CDS             <1..291
                     /gene="tilS"
                     /locus_tag="VG754_10820"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWF19754.1"
                     /translation="KSRANCEFFDADKIGSSLILRHWRPGDRFQPIGMGFSVKLQDLF
                     MNQKILRSQRHRLIVGATSKGEVYWVEGLRLAERFKLDKKTIRVLKWCWDRV"
     gene            358..2376
                     /gene="ftsH"
                     /locus_tag="VG754_10825"
     CDS             358..2376
                     /gene="ftsH"
                     /locus_tag="VG754_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWF19755.1"
                     /translation="MSDENRNTDDDRQKKSGDFKMPSRSLIVWILLLCGVILIVLVKN
                     QMATPGDLISQHRFNELVDSNMIAEATIKFNPQSPYSKEIVGTYYRMNKDDKKETVPF
                     WTSVYLTDNDISSLLDRENFKKQEPNTMLLSILWSALPLLIIGFLIWFFFIRQIKMAG
                     KGALSFGKSKARMLAKEKNKTTFKDVAGVEEAKEEVSELVEFLKDPKKFQKLGGRIPK
                     GVLMVGAPGTGKTLLAKAIAGEADAAFFSISGSDFVEMFVGVGASRVRDMFEQARKNT
                     PCLIFIDEIDAVGRSRGHGLGGGNDEREQTLNALLVEMDGFDTQEGIIIIAATNRPDV
                     LDPALLRPGRFDRQITVNLPDVRGREAILKVHAKNVKLDPVVDLGIIARGTPGYSGAE
                     LANLLNEAALLAARTGRKAVGMLELEEARDKVRWGRERRSMAMTDEDKKFTAWHEAGH
                     ALVNVMLTHTHPLHKVTIIPRGQALGSTMYLPKDDILNRRRKEMLDIIAVTMAGRIAE
                     EIVSDDISSGATGDIQQATQMARAMVTQWGMSEKLGMIQYGENNEYVFLGREMARSKD
                     YSEQTAVDIDSEVKRIIDSSFKVANDIIVNNRDKLELIANALLEYETLEGGQVEEIVR
                     TGKFTPPPPTPKVEPPSGAQAATPLPEIPPKPVPPHLPGLGSPAPAAA"
     gene            2554..>2809
                     /locus_tag="VG754_10830"
     CDS             2554..>2809
                     /locus_tag="VG754_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWF19756.1"
                     /translation="MLPMIIAPSLLAANFGRFASEAARVDKAGADWLHLDIMDGHFVP
                     NISFGPQVVRAVRPFAKLFFDAHLMCSRPEILLEPFAAAGV"
BASE COUNT          646 a          746 c          801 g          616 t
ORIGIN      
        1 aaaagccggg cgaattgcga gtttttcgat gccgacaaga tcggttcgag cctgattttg
       61 cgtcactggc gtcccggaga ccgttttcag cccattggaa tgggcttttc cgttaagctg
      121 caggatttat tcatgaatca aaaaatcttg cgctcccagc gacatcggtt gattgtgggg
      181 gccacttcca agggcgaagt gtattgggtg gaagggctga gattggccga acggttcaag
      241 ctcgacaaaa agaccatccg cgtgttgaaa tggtgttggg atagggtgta ataaacgaag
      301 ttgttgtaaa accacccgcc atgtcagcaa gcttggcgcg aacataatac caattaaatg
      361 tcagacgaaa atagaaacac agacgacgat cgccagaaaa aaagcggaga cttcaagatg
      421 ccatcgcgca gcttgattgt ttggatcctt ttgctttgcg gcgtgatttt aatcgtgctg
      481 gtcaaaaacc agatggccac tccgggagac ctgatcagcc agcaccgctt caatgagttg
      541 gtggattcca acatgattgc cgaggccacc atcaagttca acccgcagtc cccttattcc
      601 aaggaaatcg taggcaccta ctatcgcatg aataaggacg acaaaaagga aaccgttccg
      661 ttctggacca gtgtttacct gaccgacaac gacatcagtt cgttgctcga ccgcgagaat
      721 ttcaagaagc aggagccaaa caccatgctg ctgagcatcc tctggagcgc gctgccgcta
      781 ttgatcatcg gttttctcat ctggttcttt ttcattcgcc agatcaaaat ggccggaaag
      841 ggcgcgttga gctttggcaa gagcaaggca cgcatgctcg ccaaggaaaa gaacaagacc
      901 accttcaagg acgtcgccgg cgtcgaggaa gcgaaggagg aggtttctga actggtggaa
      961 ttcctcaagg atcccaagaa atttcaaaaa ctgggcggtc gcattcccaa gggcgtcttg
     1021 atggtcggcg ctcccggcac cggcaagacc ctgctggcca aggcgattgc cggcgaggct
     1081 gatgccgcat tcttcagcat cagcggctcg gatttcgtgg aaatgttcgt gggtgtcggc
     1141 gccagccgcg tgcgtgacat gttcgaacaa gcccgcaaga acacgccctg tttgattttc
     1201 atcgacgaaa ttgatgccgt cggccgcagc cgcggacatg gtttgggtgg cgggaacgac
     1261 gaacgcgaac agacgttgaa tgccttgttg gtggagatgg atggtttcga tacccaggaa
     1321 gggatcatca tcatcgccgc aaccaaccgg cctgatgtgc tggaccctgc gttattgcgc
     1381 cccggccgtt tcgaccggca aatcacagtc aatcttccgg atgtgcgcgg acgcgaggcg
     1441 attctcaaag tgcatgccaa gaacgtcaaa ctggacccag tcgtggacct gggaatcatt
     1501 gcccgcggca cgcccggtta ttccggcgct gaattggcga atcttttgaa tgaagccgcg
     1561 ttgctggccg ctcgcaccgg gcgcaaagcc gtcggcatgt tggagcttga agaggcccgc
     1621 gacaaagtgc gttggggacg cgaacgccgc agcatggcca tgaccgatga ggataagaaa
     1681 ttcacggcct ggcatgaagc gggtcacgcc ctcgtcaacg tgatgctgac tcacacgcat
     1741 ccgctgcaca aggtcaccat catccctcgg ggacaagccc tgggctcaac tatgtatctg
     1801 cccaaagacg acatcttgaa tcgtcgtcgc aaggaaatgt tggacatcat tgccgttacc
     1861 atggccggac gaattgcgga ggagattgtc tccgatgaca tttcttcagg cgcgaccgga
     1921 gacattcagc aggccaccca aatggcccgc gccatggtca cccagtgggg catgagcgag
     1981 aagctcggca tgatccaata tggcgagaac aacgaatatg ttttcctcgg acgggaaatg
     2041 gcccgcagca aggattacag cgagcagacc gcggtggaca ttgacagcga ggtcaagcgt
     2101 atcatcgaca gctctttcaa ggtcgctaat gatatcattg tcaataaccg cgataagctg
     2161 gagttgattg ccaatgcctt gctggagtac gagaccctgg agggcggtca ggtggaggaa
     2221 attgtccgca cgggcaagtt caccccgcca ccgccgaccc cgaaggttga accgccttcc
     2281 ggcgcccagg cagcgacgcc tttgccggaa attccgccca agcccgttcc gccccatttg
     2341 cccgggctcg gctcgcccgc gccagccgcc gcttaatcgg ctcgttgggt tttgcaggtt
     2401 ttcaagaaat gcggacttcg gtccgcattt cttttatttt gggctaatgt aaaggggagg
     2461 gaatatgaaa ctgaacattt tgttgccgaa tccgcaaatt cgtcacgtga ggtgttttgc
     2521 cgtgattgac tccaaggcgg tcaattcatt agcctgttgc ccatgattat cgcaccatcc
     2581 ttgttggccg ccaatttcgg ccgctttgcc agcgaggcgg cgcgcgttga caaggcgggc
     2641 gccgattggt tgcatctcga tatcatggac gggcatttcg tccccaacat atcgtttggt
     2701 ccgcaagtgg tgcgcgcggt ccgccctttc gcgaaattgt tcttcgatgc gcatttgatg
     2761 tgttcgaggc ctgagatact gttggaacct ttcgccgctg cgggcgttg
//
LOCUS       DASZMM010000449         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_998674, whole genome shotgun sequence.
ACCESSION   DASZMM010000449 DASZMM010000000
VERSION     DASZMM010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998674"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(418..1992)
                     /locus_tag="VG754_10835"
     CDS             complement(418..1992)
                     /locus_tag="VG754_10835"
                     /inference="COORDINATES: protein motif:HMM:NF040163.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5722 domain-containing protein"
                     /protein_id="HWF19757.1"
                     /translation="MILLAIFAAANVSTSSFGGEITHISATTNLITISGRNDSKSFLG
                     IAEFAPYQSSNDLASGLVAAQAKPGHSFKIRIPRFDGQRDRLYSGFLAFYTTTNNLRI
                     PEDSIHFVDDMRGVSKYNEPIPRVASKKGLQVQMVDDAIALGVKHAALNLDLTHLIDP
                     TDNAKNPSWQMDGITYHFNRGQVENLDGRVKPLSDAGVVVSLILLCYQSGDPALDRIM
                     LPPDFSPNAPHHLSAFNTVTPDGLRYFKASIEFLADRYSQPKHPHGRIVNFIVGNEVN
                     SHWEWANLGDASMEKFADDYLRTVRICNTAVRKASSTARVYISLEHHWNIRFDGPLHS
                     FCGREFIDYFNARAKAGGDFDWNLAFHPYPENLFEPRTWNDKTATFKDDTPRITFKNL
                     EMLPRYFKRKELLYYREPRRIILSEEGFDTPKTPDGETVQAAAYCYAYYKVSRIPGID
                     SFILNRHVDHRDEGGLNLGLWRRKLDSASPCEPAGKKVIYEVFREADTPQWREAFAFA
                     LPIIGIHDWKEIAPKN"
     gene            complement(2061..3038)
                     /gene="accD"
                     /locus_tag="VG754_10840"
     CDS             complement(2061..3038)
                     /gene="accD"
                     /locus_tag="VG754_10840"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HWF19758.1"
                     /translation="MNPQLSSPSMGTTSFTKKTLGLENGEPGVTPPPVSAETTFVKKP
                     KMSGGKSRKRDIPEGLWTKCKKCSTMVFDKELDENLKVCPHCQYHFPIGARERIHSLV
                     ETCSFEEMDADMTSVDVLNFTGVSSYVSKLADHQKATGQKDAVVTGFGKIDEHRVALG
                     VMDFMFLGGSMGAVVGEKLTRLVEQATEKGLPVIIISASGGARMYEGMFSLMQMAKTC
                     GALAYHAKARLPFISILTDPTTGGVTASHASVGDLILAEPHAMICFAGPRVIKDTTQA
                     ELPAGFQTSEFLLEHGLIDAIVSRKQMKKQLVEYLDFLTTGQKGATAAV"
BASE COUNT          775 a          848 c          992 g          799 t
ORIGIN      
        1 agttgccatg agagcggcga cgctttgcgc gatggagtca ttggccgccg aagtgccatc
       61 aatgccgcaa acggggcagg cgatggcgta gggcatctgg ccctgcacag gctcgacatc
      121 gaatgcataa cgttgaccgc aaccacattg gattttgatg ggaaccataa tgatattgga
      181 tgtgtcagct ctttttttga cggactggga gttcgtgcga cttggtcaac tctctggaaa
      241 actgcggatg atgctggaca cactcgtcaa tcctgtcaag aaaacagttc atctccgaca
      301 acgcacatct tacaaatcgt ggagcgcggc tgtgcgcgaa gcgccagctg cagcaacacc
      361 cgaaatcctg agttccaagc tgctgcgact ggttctgtgg acacagtcgc gctccgttca
      421 gttctttggc gcaatctcct tccaatcatg gatgccgatg atgggaagcg cgaaggcgaa
      481 agcttcgcgc cattgcggag tgtcagcttc gcggaatact tcgtagatga cttttttacc
      541 ggcgggttcg cacggagatg cgctgtccaa tttccttcgc cacaggccga ggttcagtcc
      601 gccttcatca cgatgatcga catggcggtt cagaatgaac gaatcaatgc cggggatgcg
      661 cgagactttg taataagcat aacaataagc agcggcttgc acggtttcgc cgtcgggagt
      721 tttgggcgtg tcgaaacctt cctcggagag aattattcgg cgcggctcac ggtagtaaag
      781 aagttccttg cgtttgaaat atcgcggcag catttccaaa tttttaaagg tgatgcgagg
      841 cgtgtcgtcc ttgaaggtcg ccgttttgtc gttccacgtg cgcggctcaa aaagattttc
      901 tggataggga tggaatgcaa ggttccaatc gaaatcgccg ccagccttgg cgcgcgcatt
      961 gaagtaatca atgaattcgc ggccgcaaaa agagtgcaac gggccgtcga agcggatgtt
     1021 ccaatggtgc tccagcgaaa tatagacgcg cgcggtggaa ctggccttgc gcacggcggt
     1081 gttgcagatg cggacggtgc gcaaataatc gtcggcgaat ttttccatcg aagcatcgcc
     1141 gaggttcgcc cattcccaat gcgaattgac ttcgttgccg acgatgaaat tgacaatgcg
     1201 gccgtgtgga tgctttggct gcgaataacg gtcagcgagg aattcaatgg aagccttgaa
     1261 ataacggagg ccgtcgggag tgacggtgtt gaacgccgaa agatgatgcg gcgcattggg
     1321 actgaaatcg ggagggagca tgatgcggtc gagcgcggga tcgccacttt gatagcacag
     1381 gagaatgagc gacacgacaa cgccggcatc ggagagaggc ttgacgcgtc catcgagatt
     1441 ttcaacctgc ccacgattga aatgataggt aatgccatcc atttgccagg acggattctt
     1501 ggcattgtcg gtgggatcaa tcaggtgcgt caggtcgaga ttcagcgcgg cgtgcttcac
     1561 accgagcgca atcgcgtcat ccaccatctg cacctgcaaa cctttcttgc tggcgactcg
     1621 cggaatgggt tcgttgtatt tggagacacc gcgcatgtca tcgacgaaat ggatggaatc
     1681 ttccgggatg cgcaggttgt tcgttgttgt gtagaatgca agaaagccgg agtaaagacg
     1741 gtcgcgttgt ccatcgaagc gaggaattcg gattttgaaa ctgtggcccg gtttggcttg
     1801 cgccgcgacc aggccgcttg cgaggtcatt gctggattga tagggagcga attcggcgat
     1861 gccgaggaac gacttgctgt cgttgcgtcc gctgatggtg atcagattcg tggttgcgga
     1921 gatgtgggtg atttcgccgc cgaaactgct ggtcgatacg ttggctgcgg cgaaaatagc
     1981 gaggaggatc aaaagcgatg aaaaccgcaa ccgaagagaa tcgaagaatg acatttaaag
     2041 agtctgaaaa taggaaaggt tcagactgcg gcagtggcac ctttttgccc tgtagtcagg
     2101 aaatccaaat attcgacaag ctgttttttc atttgcttgc gggaaacgat ggcgtcaatg
     2161 agcccgtgtt cgagcagaaa ttcggaggtt tgaaaaccgg cgggaagttc agcctgggtg
     2221 gtgtccttga tgacgcgcgg gccggcgaaa caaatcatgg cgtgtggttc ggcgaggata
     2281 aggtcgccca cgctggcgtg gctggccgtc acgccgccgg tggtgggatc ggtgagaatg
     2341 ctgatgaacg gcagcctggc ttttgcgtga taagccagcg caccgcaggt cttggccatt
     2401 tgcatcaggc tgaacatgcc ttcgtacatg cgcgccccgc cgcttgcgga aatgatgatg
     2461 actggcaatc ccttttccgt cgcctgctcc acaagccgcg tcaacttctc gcccacgacc
     2521 gcccccatcg aaccaccgag aaacatgaaa tccataacgc ccaaggcaac gcgatgttca
     2581 tcgatttttc caaacccggt gacgaccgcg tctttctggc cggttgcttt ctggtgatcg
     2641 gcgagtttgg atacataact ggagacgccg gtgaagttga gcacgtccac gctcgtcatg
     2701 tcggcatcca tctcttcgaa cgaacaagtc tcgacaagcg aatggatgcg ttcgcgcgcg
     2761 ccgatgggaa agtgatactg gcaatgcggg cagaccttga ggttttcatc cagctccttg
     2821 tcgaaaacca tggtgctgca tttcttgcac ttggtccaaa ggccttcggg gatgtctcgt
     2881 ttgcgggact tgccgccgct catcttgggt tttttgacga aggtggtttc ggcgctgacc
     2941 ggaggcggag tgacgcccgg ttcgccattt tccaagccaa gggttttctt cgtgaacgag
     3001 gtggtgccca ttgaaggcga tgaaagttga gggttcattg gtgagggttg gatgccagcc
     3061 tttttacgcg ttttgcggtt tttgtttttg gagcattctt cacaaacgtc catcttctgc
     3121 attttgtccc caacgatttg ggtcaagaat acagtggctt ctctttcctg acaaatggaa
     3181 cacaacataa tcgtatcaac ccgccgcggc aaacagccga gtcgagtacg acttgttttt
     3241 acaaatccaa tcaaagttca tcattttatg ttttcaaaga ccccgggcca cggtccaaac
     3301 gcaaacatcg tccgcacccg aagacagcaa tactttggca caagcactgg tggttgcgcc
     3361 agtcgtaaaa acatcatcca acaccacgac tcgctggcca ttcagcttct cacc
//
LOCUS       DASZMM010000450         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4863780, whole genome shotgun sequence.
ACCESSION   DASZMM010000450 DASZMM010000000
VERSION     DASZMM010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4863780"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VG754_10845"
     CDS             complement(<1..789)
                     /locus_tag="VG754_10845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417685.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWF19759.1"
                     /translation="MSNLLQEIEAQISGVKTSVARQNVGTVREIGDGVAKIEGLTDAM
                     LNEMLDFGNGVTGLALNLEETEVGAIILGDYTSIEEGQEVRTTGKLLQVPVGKGLLGR
                     VVNALGQPVDGKGPIKAETNYPVEKIAPGIIKRRGVSQPVQTGIMAVDAMIPIGRGQR
                     ELIIGDRSTGKTTIGVDTMINQARINKAGEASGDKDFRPIYSIYVAIGQKQSNVARVI
                     NVLEEAGAMPYTIVVVSAASDSATNQYLAPFAGTAMGEWFMDHGM"
     gene            complement(812..1204)
                     /locus_tag="VG754_10850"
     CDS             complement(812..1204)
                     /locus_tag="VG754_10850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HWF19760.1"
                     /translation="MKISKQARRDAKQLFRSCFNNGVLDENRVRQTVQAVIAQKPRGY
                     VAILSQFARLVKLDQERRTARVESAVALVPDQQAAVQTNLTRKYGPGLNVTYLQNPAL
                     LGGLRIQVGSDVYDGSVRARLESLREAF"
     gene            complement(1214..1765)
                     /gene="atpF"
                     /locus_tag="VG754_10855"
     CDS             complement(1214..1765)
                     /gene="atpF"
                     /locus_tag="VG754_10855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HWF19761.1"
                     /translation="MNHLVILANIGSDLVETGKSTAKQFGVDWPHLVSQIISFCIVAA
                     LLYFFAYKRILQVLEERRQRIAEGLANADKIKAELARTEAARLEVLNQASAQANKLIE
                     DARAAAARVQEQETQKAIAAAEQIIAKAREAAAQDHERMLAELRREVGRLVVETTSKV
                     TGKILTPDDQKRLADETSRQLAA"
     gene            complement(1823..2044)
                     /locus_tag="VG754_10860"
     CDS             complement(1823..2044)
                     /locus_tag="VG754_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HWF19762.1"
                     /translation="MLMPMLAEISGNIHVGLAALGAALGVGFIGMKASEAVGRNPGAA
                     TPILVQSILAIAFAEAIVFYALFLTKWGP"
     gene            complement(2117..2992)
                     /gene="atpB"
                     /locus_tag="VG754_10865"
     CDS             complement(2117..2992)
                     /gene="atpB"
                     /locus_tag="VG754_10865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWF19763.1"
                     /translation="MLAFAPLAGWSAEPQPTSPQAAAHEEHGVPATAGELWHIGDFII
                     TNSMVVTWIVAAALIILAQLATRNIQQVPSGLQNFWEWMVESLHNFLEGIIGHQLVQR
                     TFWFFASIFIFICFANWSGLIPGFGTIGYGERTDHGFRVEQPLFRGANADLNMTLAMA
                     LVFFACWIYWALQANGPLGFLKHLFAPKGESKGWLKPLLVVVFFAVGFLEIFSILFRP
                     ISLSFRLYGNIFAGENMLEAMSNLVPHLGWLLPIPFYFMELLVGVIQALVFMLLTAVF
                     TMLIMPHDEGHEAHH"
     gene            complement(3272..3811)
                     /locus_tag="VG754_10870"
     CDS             complement(3272..3811)
                     /locus_tag="VG754_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19764.1"
                     /translation="MKNFHQNLLIILALALCGLCIWQWYGQTLQRNQTDTLNQLLNQK
                     LAAIQQYTNSIRTMDGQIAQMDARITELKGTIKTNEQVILDQKRELNGLDATNQTLTL
                     QIGEYKDGVAKLEQKLKEAYDGIQKQNDAIKQLTAQREEFVKKLNDSVKDRNDIVSKY
                     NELATQYEKLQGSSKSSGK"
     gene            complement(3815..>4044)
                     /locus_tag="VG754_10875"
     CDS             complement(3815..>4044)
                     /locus_tag="VG754_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWF19765.1"
                     /translation="VDKFGQVEMDKKVVPLAELQSVLDERRKANANVPVYITGTRDAT
                     HGSVGSVLDFVKRAGIDRVAIGVKPPDAGNP"
BASE COUNT          928 a         1118 c         1038 g          960 t
ORIGIN      
        1 catgccatga tccatgaacc attcgcccat tgccgttccg gcaaacggag ccaaatattg
       61 attcgtcgca gaatccgaag cggcggaaac aacgacgatg gtataaggca ttgcaccggc
      121 ttcttcgagc acgttgatga cccgcgcgac gttggactgc ttctggccga tcgcaacgta
      181 aatgctgtag atgggacgga agtccttgtc gccagaagct tcgccagctt tgttgatgcg
      241 ggcctggttg atcatcgtgt ccacgccaat ggtcgtcttg ccggtggaac gatcgccgat
      301 gatcaattcg cgttggccgc gaccaatggg aatcatcgcg tccacagcca tgatgccggt
      361 ttgcaccggc tggctgacgc cacggcgttt gatgatgccg ggcgcgattt tttcgacggg
      421 atagtttgtt tcagccttga tgggaccttt cccgtcaaca ggctggccaa gcgcattgac
      481 cacgcggccc aaaagacctt tgccgaccgg cacctgcaac agcttgccgg tggtgcggac
      541 ttcctggcct tcctcaatgc tggtgtaatc gccaagaatg atggcgccaa cttcagtttc
      601 ttcgaggttc aacgccagcc cggtgacgcc gttgccgaag tccaacatct cgttcagcat
      661 ggcgtcggtc aggccttcaa tcttggccac gccgtcgccg atttcacgga cggtgccgac
      721 gttctggcgg gcgaccgaag ttttaactcc ggaaatttgg gcttcaattt cttgcaataa
      781 gttgctcata gatcagtcaa tcaagttacg attaaaatgc ttcgcgcaaa ctttccaatc
      841 gcgcgcggac gctgccgtca tagacatcgc tgccaacctg gatacgcaag ccgcccagca
      901 aggcagggtt ttgcaagtag gtcacgttca gtcccggacc gtatttgcgg gtcaaattgg
      961 tttgcacagc cgcctgctgg tcgggcacga gggcaactgc gctttcaacc cgcgccgtgc
     1021 ggcgctcctg gtcaagcttc accaaacgcg cgaattgcga aagtatcgca acgtacccgc
     1081 gcggcttttg cgcgatgacc gcctgcaccg tttgacgcac gcggttctcg tccaagactc
     1141 cgttgttaaa acaggagcgg aacagttgct tggcgtcgcg gcgggcttgt ttggaaattt
     1201 tcattcagcc aaattaagct gccaattgcc ggcttgtttc gtccgccaga cgcttctggt
     1261 cgtccggcgt gaggattttt ccggtcacct tgctggtggt ttccaccacc aagcgaccga
     1321 cttcccggcg caactcggcc aacatgcgct catggtcctg ggcggcggct tcgcgggcct
     1381 tggcgatgat ttgctcggcg gcagcgatgg ctttttgcgt ttcctgctcc tgaacgcgcg
     1441 cggcggccgc gcgggcatcc tcaatcaact tgttcgcctg cgcgctggcc tgattcaaca
     1501 cctcaagacg cgcggcttcg gtgcgggcga gttcagcctt tatcttgtcg gcgttcgcca
     1561 aaccttcggc gatgcgctga cggcgttctt cgagcacttg aagaatacgc ttgtaggcaa
     1621 agaaataaag caacgcggcg acgatgcaaa agctgatgat ctgcgaaacc aaatggggcc
     1681 aatcaacgcc aaattgcttt gccgtacttt tccccgtttc aacgagatca ctgccaatat
     1741 ttgccaaaat aaccaaatga ttcataaact gaattgttgc aatgggcgcg gagccattaa
     1801 cgctccgcgc ccaataatta gactaaggtc cccatttcgt gaggaacagg gcgtagaaca
     1861 caatggcttc tgcgaaggcg attgcgagaa tcgactgcac cagaatcggc gtcgcagcgc
     1921 cgggattgcg acccaccgct tcgctcgcct tcatgccaat gaaacccacg ccgagggccg
     1981 cgccgagcgc tgccagtccg acatggatat ttccactgat ttctgccaac atgggcatca
     2041 acatatttgt ccttttcttt ggttgctcgc cggcttccgc gattgaacac ggtcaaaccg
     2101 gcgaaattca aaatcatcaa tggtgcgcct cgtgcccctc atcgtgcggc atgatcagca
     2161 tcgtgaacac cgccgtcaaa agcataaaca ccagcgcctg gatcactcca accaggagtt
     2221 ccatgaaata aaatggaatc ggcaataacc aacccagatg tggaactaga ttggacatcg
     2281 cctccagcat gttttcgccg gcaaaaatat ttccgtaaag acggaagctc aacgaaatcg
     2341 gacggaacaa gatggaaaaa atttccagga agccaaccgc aaaaaacacc acgacaagga
     2401 gcggtttaag ccagcctttc gattcaccct tgggcgcaaa caaatgtttc aggaaaccta
     2461 gcgggccgtt ggcctgaagc gcccaataaa tccagcaggc aaagaacacc agcgccattg
     2521 ccaaggtcat gttcaggtcg gcgttagcgc cgcggaacaa tggctgctca accctgaacc
     2581 catgatctgt acgttcgcca tatccgatcg tgccgaaacc gggaatcaat ccactccagt
     2641 tggcgaaaca gataaagatg aagatgctcg cgaaaaacca gaaagtcctt tgcactaatt
     2701 gatggccgat aatcccttcc aaaaaattgt gcaggctctc gaccatccat tcccaaaaat
     2761 tttgaaggcc ggaagggact tgctggatgt tgcgcgtcgc aagctgcgcc aggatgatga
     2821 gagcggccgc gacaatccag gtgacgacca tcgaattggt tatgatgaaa tctccgatat
     2881 gccagagttc gccggcagtt gcgggcacac catgttcctc atgggcggct gcttgaggcg
     2941 atgttggttg cggttccgca ctccaccccg ccaacggggc gaaggcgagc atcactatta
     3001 gaattgcttt aacaaaacgc attgcgttca aaattgtgtc tggattccgg ggacacccgt
     3061 cccctgtcga acccggcttg tgcctgaaag ctgggaaaaa gtgccttatc gtgaaaaatt
     3121 tcacaagcgt tttcttaaaa taaattatga acgctcgcct tattagagag acttcgaagc
     3181 gggcccataa atggttgctc caagctccga ttaggaagga atcaagacaa aatccattag
     3241 ggaaacttac cgatttgact gatgactgca ctcacttgcc ggaagatttt gaactacctt
     3301 ggagcttttc atattgggtg gccaattcat tgtatttgct gacaatgtca ttccgatctt
     3361 tcacgctatc gttcagcttt ttgacaaatt cctcccgttg cgcggtcagt tgcttgatgg
     3421 cgtcattttg tttttgaatc ccatcataag cttccttgag tttctgctcc aactttgcca
     3481 ccccatcttt gtattcgccg atttgaagcg tcaacgtctg gttcgtcgca tcgagtccgt
     3541 tgagttcccg tttttgatcc aaaatcacct gctcgttggt tttgatggtt cccttcaatt
     3601 ccgtgattct cgcgtccatc tgggcaattt ggccgtccat ggtgcgaatc gaattggtgt
     3661 attgctgaat ggcagccaat ttttgattga gcaactggtt cagagtgtcc gtctggtttc
     3721 gttgcaaagt ttggccatac cattgccaga tacacagtcc gcaaagagcg agcgcaagaa
     3781 tgattagcag gttttgatga aaatttttca tggtttaagg attaccggca tcgggcggct
     3841 tcacgccaat cgccacacgg tcgatgccag cgcgcttcac aaaatcgagc acagagccaa
     3901 ccgaaccgtg cgtggcatcg cgcgtgccgg tgatgtaaac gggcacgttg gcgttggcct
     3961 ttcgacgttc atccagcacg ctttgcaact ctgcaagggg aaccactttt ttgtccatct
     4021 caacctgccc gaacttatcc accg
//
LOCUS       DASZMM010000451         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_488283, whole genome shotgun sequence.
ACCESSION   DASZMM010000451 DASZMM010000000
VERSION     DASZMM010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488283"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VG754_10880"
     CDS             complement(<1..1120)
                     /locus_tag="VG754_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWF19766.1"
                     /translation="MDREKIDGWCEKGILGLVLGILIFGPLALGAHGTWQFLVLQALT
                     MGVMALWALRMWVSPSPKLLWPPICWAVLAFMLYAIVRYLQADIEYAAREELIRVLIY
                     GFLFFAILNNLHRQEAIQIVTVTLLFLGMAISMYAVWQFFTKSQKVWNLENPYKGRAG
                     GTFIYPNHLAGFLELLVPLGLCQVLMGRSGHVFKVVLGYASFVMLVGIGVTISRGGWA
                     VTALTLLVFSLVMLAQRDYRMQGVVLMAVLFIAGALAVPRVETMQQRVKQIRSSGRAD
                     DLRLSIWEPAVQIWKEHFWWGAGPNHFDYRFPEFRPTSIQRRPFKAHNDYLNTLVDWG
                     VVGTALVASAWVLLYWGIIKVWRDVRGRRDDFSRKKSNK"
     gene            complement(1338..1574)
                     /locus_tag="VG754_10885"
     CDS             complement(1338..1574)
                     /locus_tag="VG754_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWF19767.1"
                     /translation="MSTLTIQLPDSLKKTIEELAAREGYTVSQFLASAAGEKLAVVLT
                     MDYLRREAAAGRREDFDKYLAAIPDVPPLPGDEK"
     gene            1567..1920
                     /locus_tag="VG754_10890"
     CDS             1567..1920
                     /locus_tag="VG754_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19768.1"
                     /translation="MLMLFSGAGSKIHAESAPQCQVAPSHENLSHYASQCGGNLRQMP
                     VPKPYHHRNLAGMAAKENEFLASESDPRQTVEIAKRLPPGDRVEDSQAGLTLAAIQYK
                     GPPTDKSRISQKEWN"
     gene            complement(2034..>2668)
                     /locus_tag="VG754_10895"
     CDS             complement(2034..>2668)
                     /locus_tag="VG754_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWF19769.1"
                     /translation="KNGIFEFDEKTKELVDFRTKKNGTKSSYEPVARDKDEKFMYELV
                     VDLSKLEPTVACHPDPGQRKKAKEMGNVTLDRAYIGSCTGGKTSDFLEFARVLRGKRV
                     KIDTFGVPATPEIVHDLQTAKWGEKTVWQILVDSGVQMTENAGCAACLGGPVDTFGRM
                     NSPMKCISATNRNFPGRMGHKESQVFLASPATVAASAITGKITDPREYWS"
BASE COUNT          620 a          825 c          671 g          552 t
ORIGIN      
        1 atttgttgct ttttttccgc gaaaaatcat cccgcctgcc gcgcacgtcc cgccacactt
       61 tgatgattcc ccaatacaac aacacccatg ccgatgccac caaggcggtg ccgacgacgc
      121 cccaatccac aagcgtgttc aggtaatcgt tgtgcgcctt gaacgggcgg cgctggattg
      181 aggtcgggcg aaattccggg aagcgataat caaaatggtt cggccccgcg ccccaccaaa
      241 aatgttcctt ccagatctgc accgccggtt cccagatgga aagccgcagg tcgtccgccc
      301 tgccgctgga ccttatctgc ttcacgcgct gttgcatcgt ttccacgcgt gggacagcca
      361 aagcgcccgc aatgaacaac actgccatca agaccacgcc ttgcatccgg taatcccgtt
      421 gcgccagcat caccaggctg aacaccagca aggtcaacgc cgttacggcc catccgccgc
      481 gcgaaatcgt gacaccaatg ccgaccagca tcacgaacga cgcatagccg agcacaacct
      541 tgaacacatg gcccgaacgt cccatcaaca cctggcacag accgagaggc accagcagtt
      601 ccaaaaatcc cgcgagatgg ttcggataaa tgaacgtccc gcccgcgcga cccttgtaag
      661 gattttccag gttccaaact ttttgcgatt tcgtgaaaaa ttgccagacc gcatacatgg
      721 aaatcgccat gccgagaaac agcagcgtga cagtgacgat ctggatggct tcctgccggt
      781 gaagattgtt gaggattgcg aaaaacaaaa aaccgtagat gaggacgcgg atcaattcct
      841 cgcgcgcggc gtattcgatg tcggcttgca gatagcggac aatcgcatag agcatgaagg
      901 cgagcaccgc ccagcaaatc ggcggccaga gaagtttcgg gctgggcgaa acccacatcc
      961 gcaacgccca aagcgccatc acgcccatcg tgagcgcctg caagaccagg aattgccacg
     1021 tcccatgcgc gcccaaagcg agcggcccga aaatcaggat tcccagcacc agtccgagaa
     1081 tccccttctc gcaccaccca tcaatttttt cacggtccat tcaatataaa catggagtgt
     1141 tggagtgatg gagtattggg cagaacagca gcactccatt actccaattc tgcaattcca
     1201 caaccaccag cggcgctagc ccgcgggctg ttccaactcc gcactccgca ttccgcattc
     1261 cgagttccgc acttccttca gctccaatat tcgcgcgggg cggtgatttt tctgcacgag
     1321 ttggccaagt ttaatgttca tttttcatcg ccgggcaatg gcggaacgtc tggaatggca
     1381 gcgaggtatt tgtcgaaatc ctcgcggcga ccggcagccg cttcgcggcg cagataatcc
     1441 attgtcagca cgacagccag tttctctcca gcggcgctgg ccagaaactg actgacagta
     1501 taaccctcgc gagccgcaag ctcctcaatt gtttttttca gcgagtccgg cagttggatc
     1561 gttagcgtgc tcatgttatt ttccggagca ggttcaaaaa ttcacgcgga gtcagcgccg
     1621 caatgccaag ttgctccgag ccatgaaaat ctttcacatt atgcgtcaca atgtggcggc
     1681 aacctgcggc aaatgccagt tccaaaacca tatcatcatc ggaatctcgc aggaatggcc
     1741 gccaaagaaa atgaatttct tgcaagtgag agcgacccac gacaaaccgt agaaatcgca
     1801 aaacgtcttc cgcccggaga ccgggtggaa gattctcagg ccgggttaac acttgctgcc
     1861 attcagtata aagggccacc gacagacaaa tctcgaattt ctcaaaagga atggaactga
     1921 cgagagcatg gcttgctccg gtccgggacc tggctgccgc aaccatgacg ctcgtgtcaa
     1981 aaacaaccct catataatcg agtcccaccg ccaagcgttc aggctggcat cactcagctc
     2041 caatattcgc gcgggtcggt gattttgccg gtgatggcgc tggcggcaac cgtcgcgggt
     2101 gacgcaagga acacctgcga ttccttgtgg cccatgcggc cggggaaatt gcggttcgtc
     2161 gcgctgatgc atttcatcgg cgaattcatc cggccgaacg tgtccaccgg cccgccgagg
     2221 cacgcggcgc aaccggcgtt ttcggtcatc tgcacgcctg aatccacgag gatttgccag
     2281 accgtttttt cgccccactt cgccgtttgc aaatcatgaa cgatttccgg cgtggccggc
     2341 acgccgaacg tgtcgatctt cacccgtttg ccgcgcaaca cgcgcgcgaa ttccaaaaag
     2401 tcgctcgtct tgccgccggt gcaggaaccg atgtaggcgc ggtcaagcgt cacgttgccc
     2461 atttccttcg ccttcttgcg ctggccggga tcgggatggc acgcgacggt cggttcgagc
     2521 tttgacaaat ccaccacaag ctcatacata aatttctcgt ccttgtcgcg cgcgaccggc
     2581 tcgtaactcg atttcgtccc gttcttcttc gtccgaaaat ccaccaattc cttcgtcttt
     2641 tcgtcgaatt caaaaatccc gttcttgc
//
LOCUS       DASZMM010000452         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_488761, whole genome shotgun sequence.
ACCESSION   DASZMM010000452 DASZMM010000000
VERSION     DASZMM010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488761"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..689
                     /gene="yajC"
                     /locus_tag="VG754_10900"
     CDS             342..689
                     /gene="yajC"
                     /locus_tag="VG754_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWF19770.1"
                     /translation="MSLNGWNIFLAQQAAASGNSAPTVLQFVPFILMFVVLYFIMIRP
                     QSKKAKQVAAMLKTMKPGDKIVTNSGIVGTVVSVKDRTVSIRSADTKLEVLKSAVTEI
                     TERSGETSASESQ"
     gene            710..>2865
                     /gene="secD"
                     /locus_tag="VG754_10905"
     CDS             710..>2865
                     /gene="secD"
                     /locus_tag="VG754_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWF19771.1"
                     /translation="MNRNNLWKFLFVLAVLAWSFYEMYPPVGRNLLDEFRDKAVRVDT
                     NFNAIVAQAQTLQKDHPDRTFGNLLEASSTNDLTHYFPFYDVKGELNPNRAVLYHLQQ
                     AAAGKIHLGLDLQGGTSFVVEMDTNRLEQSTNSIITQEGRTAALSQAAEVLRKRVDSV
                     GVAEPVIQPQGNNQILIQLPGLSEADTDQAKAKILKAAFLEFRLVHENSADLLKEGTT
                     TEPGYEMLKEKNTAPNGTTTITSYLVKKHPELTGEYIKSASVGRSTMGEPEIDFTLTS
                     KGADLFAQITRDNLGRQLAIVLDGELQSTPLIKTEIPNGSGQITGNYTDKQAADLANI
                     LENPLQAPVHIVSENRVDPTLGRDTVKSGVRSAVIGTILVAAFMAIYYLFAGVVADVA
                     MVTNLIILMGIMCAIPATFTLPGIAGVVLTIGMAVDANVLIFERIREESAKGKSLRGA
                     LAAGYDRAFGTIFDSHVTTLISSLILWNMGTGPIKGFGVTLTIGVAASLFTALVVTRL
                     IFDFMLSKGMLKSLNMFHIIRATKLNFMKLAVPAFILSWTIILIGLGYGYFVRGNNML
                     GISFKGGDNILLTFDKKIPSDQIASALTKDAGVFDPVVQYQKDIAGTGAEKLSITSET
                     GSAEKVQQVLKKDFPDANFRRSGLESVGPTIGKDIRDTAILASFVSLFGILVYVALRY
                     EFSFAVGAVVAVIHDVLMTIGCYCLTGLFAQHGHGR"
BASE COUNT          665 a          786 c          754 g          660 t
ORIGIN      
        1 gatccccgat gacaaagcgg actcggcatc ttgaaatgcc gagtccgcct cgttgaagct
       61 gcgattattg attaatgcag gcgctcgtcg atccagtcga tgtgccactc ggccatttgt
      121 tggaaggtgt tgctcatttt cgccatttgc aaccgtgatg aaaccggtct agattgggaa
      181 ttgcttgtcg ggcatggttt tacgcgcaag cgggctattt tggcaaaatt gggacctgaa
      241 tcaggagaga acgcgcctga aacgacttct ataaaatgcg cttataacgc ttgccaaatc
      301 cgcctacttc cttagattta acggctcact tgatttgatt tatgagtttg aatggttgga
      361 acatcttttt ggcgcagcaa gcggctgctt cgggaaactc ggcgcccacg gtgctgcagt
      421 tcgtcccgtt catcctgatg tttgtggtgt tgtacttcat catgatccgc ccgcaatcaa
      481 agaaggcaaa gcaagtcgcg gccatgctca agacaatgaa acccggtgac aagatcgtca
      541 ccaacagcgg catcgttggc acggtcgtga gcgtcaagga caggaccgtt tccatccgtt
      601 ctgccgacac caagctcgaa gtcctcaaat cagccgtcac ggaaatcacc gagcgctccg
      661 gtgaaaccag cgccagtgaa tctcaatagc aattgcaata gttcaagtca tgaatcgaaa
      721 taatctttgg aagttcctgt tcgttctcgc ggtgcttgcc tggtcgtttt acgagatgta
      781 cccgccggtg ggccgcaatc tgctcgacga attccgcgac aaggcggtgc gcgtggacac
      841 caatttcaat gccatcgtcg cgcaggcgca aaccctccag aaagaccatc cggaccggac
      901 gttcggcaat ctgctcgaag cctcaagcac gaacgacctc acccattatt tcccgttcta
      961 tgacgtgaaa ggcgagttga atcccaatcg cgccgttctt taccatttgc aacaggccgc
     1021 tgccggcaag atccatctcg gccttgattt gcaaggcggc acttcatttg tggtggagat
     1081 ggacaccaac cggcttgagc aatccaccaa cagcatcatc acgcaggaag ggcgcaccgc
     1141 cgcattgtcg caggcggcgg aagtcttgcg caaacgcgtg gactcagtcg gcgttgccga
     1201 gccggtcatc cagccgcagg ggaacaatca aatcctgatt caacttccag gtttgtcgga
     1261 ggccgacacg gaccaagcca aggcgaaaat cctgaaagcg gccttcctcg aattccgcct
     1321 tgttcatgag aacagcgcgg atcttctcaa ggaaggcaca accactgagc ccggttatga
     1381 aatgctcaag gaaaaaaaca ctgctcccaa tggcaccacg accataacgt cgtatctggt
     1441 caaaaagcat cctgaactca ctggcgagta catcaaaagc gcctctgtcg gccgcagcac
     1501 gatgggcgag ccggaaattg acttcacgct cacttcaaag ggcgcggacc tcttcgcaca
     1561 gatcactcgc gataatctcg gacgccagtt ggcgattgtg ctcgatggcg agttgcaatc
     1621 cacgccgctc atcaagacgg aaattccgaa tggcagcggc caaatcactg gaaattatac
     1681 ggacaagcaa gcagcggatt tggcgaatat tctcgaaaat cctcttcaag cgcccgtcca
     1741 tatcgtttct gaaaacaggg tggacccgac cttgggacgc gacacggtca agagcggcgt
     1801 tcgctccgcg gtcatcggca ccatcttggt cgccgcgttc atggcgattt attatctatt
     1861 cgctggagtc gtggcagacg tggcaatggt aacaaacctt ataattctga tgggaattat
     1921 gtgtgccata ccagcaactt ttacccttcc aggcatcgcg ggcgtggtcc tgaccatcgg
     1981 tatggcggtg gacgcaaacg tccttatctt tgagcgcatc cgcgaagaat cggccaaggg
     2041 caaatccctg cgcggcgcgc tggcggcggg ttatgaccgc gcgttcggca ccattttcga
     2101 ttctcacgtc acgacgctga tttcctcctt aatcctttgg aacatgggta ccggcccgat
     2161 caaaggcttc ggcgtgacgt tgactatcgg tgtcgcggca agcttgttca cggcgctggt
     2221 cgtgacgcgg ttgattttcg atttcatgct ttcgaagggg atgctgaaat cgctgaacat
     2281 gttccacatc atccgcgcga cgaagctcaa cttcatgaaa ctggccgtgc cggcattcat
     2341 cctttcatgg accatcatcc tgatcggttt gggttacggc tattttgtgc gcggcaacaa
     2401 catgctcggc atttcattca agggcggcga caacatcctt ctgactttcg acaaaaaaat
     2461 tcccagcgac cagattgcaa gcgcgctcac caaggatgcc ggagtgtttg atccggtcgt
     2521 ccaatatcaa aaggacatcg cgggtactgg cgcggaaaaa ctttccatca cctcggaaac
     2581 cggcagcgcc gaaaaggtgc aacaggtgct caaaaaagac tttcctgacg ccaatttcag
     2641 acgaagcggc ttggaatcag tcgggccgac catcggcaag gacatccggg atacggcgat
     2701 cctggccagc tttgtttcgt tgttcggcat cctcgtctat gtggcattgc gttatgaatt
     2761 ttcatttgct gttggcgcgg tggtggcagt catccacgac gtgctgatga ccattggttg
     2821 ttattgtttg accggattgt tcgctcaaca cggccatggc cgcga
//
LOCUS       DASZMM010000453         7115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1325281, whole genome shotgun sequence.
ACCESSION   DASZMM010000453 DASZMM010000000
VERSION     DASZMM010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325281"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1151)
                     /gene="purM"
                     /locus_tag="VG754_10910"
     CDS             complement(75..1151)
                     /gene="purM"
                     /locus_tag="VG754_10910"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWF19772.1"
                     /translation="MKKKAYARAGVDIDLGNRVKATLPQLLAATHRREVLGKVGGFGG
                     LFALDVKKYREPVLVSSTDGVGTKLKLAFAMDKHDTIGEDLVNHCVDDIAVLGAEPLF
                     FLDYIGTGKLKPHVFTQLIEGFARGCAKNNCALIGGETAQMPGFYQPGEYDVSGTIVG
                     VVEKNRMLDGKGIRPGDAVIGLASSGLHTNGYSLARKIFFEQMKLKPKSRVAELGNTI
                     GDELLKVHVSYGPLIQTLLKKFNAAFAKSQGSNLKFQIIKGLAHITGGGFVDNIPRVL
                     PKNCDVIIRKGTWDVLPIFQMIQAKGGVPEAELYQVFNMGIGMTIIVSGEKVDAVLKF
                     IRAQKQKAWVIGEIVKGKGEVRVQ"
     gene            complement(1222..2553)
                     /locus_tag="VG754_10915"
     CDS             complement(1222..2553)
                     /locus_tag="VG754_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWF19773.1"
                     /translation="MANILKLLGVAALVLLNGFFVAAELALVKIRDTQIETLVQGGNR
                     RARIVRRLAQNLEAAISATQFGITLASLGLGILVEPVFLSLLAPVFHWLNVESQTVQH
                     TVAILAGFFVNSFLLIVIGELAPKAIAIRKTVPVALWTARPLSWFARITFPFVWLLNR
                     SAMWLLKQIGIEPASEAQRSHSEEELRLLFAVSDRYSGATRLGRDIVLNALDLRRRIA
                     REVMRPRNEIVCLDTEASIAECLDVAEKTRYSRFPLCERGNLDKTLGVVHFKDLFAAR
                     LKARSGADLITVARKLIYVPETARLEKLLQLFLERKLHLAIVVDEYGGTVGMVTLENI
                     LEELVGQIQDEFDQEKPLLVKTGAETWELAGTLPLHELAELVREPLQEEGITTVSGWV
                     THRLGGFPKPGDVLSLGNYALQVEEMEGLRVAKLRLKLAEEGPKTGGDGVV"
     gene            complement(2567..3556)
                     /locus_tag="VG754_10920"
     CDS             complement(2567..3556)
                     /locus_tag="VG754_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HWF19774.1"
                     /translation="MMPIFLISLLFVGSVVMSFLLSGMEAGVLALSRLRIRQMLRAGN
                     PNARALLDYLERPEDFLWTILVGNMLANFIAVGLMVVLLRRWLDPYPGFFLISFLALM
                     FLFYALFELLPKMLFRMFPNRLTLAIARPFRLIHLVLSPLVKLITTVSQRFLKWTGGR
                     AFMGHLFGSREEMRLVMQESAQGLTSEERQMINRVLDLQNRTVRDIAIPMDKVATLTM
                     QTRMGDALKISQERRLSRFPVWRGEGNERKIVGLVSLRAVVYQAELDPEKTAGDYVRP
                     ALYMEENMRLEEALKRMQRSGQRLAIVLGHDQREAGIVTLQDILKVIFGEVRL"
     gene            complement(3561..4796)
                     /locus_tag="VG754_10925"
     CDS             complement(3561..4796)
                     /locus_tag="VG754_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWF19775.1"
                     /translation="MALMPYLAGVGLLVFAGMSFFFALAESALFSLGKWQLRQLAERS
                     PNAGGLVARLLAEPQHLLATIVLGNTVANASILAITLWAAPWGPLLWFVCLPALLVLI
                     LLGCEVAPKTLAVRSPELWALRVARPMLFLQKFTGPFRRVAQRLDNAILNALVPQSIK
                     PGVAMSDEEYQELLELAFQQGTLAGTEKEIILQIIGLDRQTAQDVMKPRAQMAAISDD
                     LPVEEMIAAARKFKHTRLPLYDETLDTIVGVLNARALLWNPEMDLADAIEFPSFVPES
                     MNLLQLLKSLQRQQRGLAIVLDEFGGTAGIVTMQDILEEMVGEIRGEGEPEGFVIEKL
                     GVGRWRVNGTMRLEDFRREYPALGEVSEVDTMGGLMVALHETIPAVGDAVTFRGLKLT
                     AQSADERRVRELMVETLRR"
     gene            complement(5056..6384)
                     /gene="ftsZ"
                     /locus_tag="VG754_10930"
     CDS             complement(5056..6384)
                     /gene="ftsZ"
                     /locus_tag="VG754_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWF19776.1"
                     /translation="MQSETNQLAETGFVAKKLTLKVFGVGGGGGNAVDYMARQDFDGV
                     SMMAINTDSKALSQLSTVERMTLGAKLTRGLGTGGDPEMGRAAAEEDLEKIRSLCAGA
                     DIVCVVAGLGGGTGTGAGPVVARLAKETGALVLGIVTLPFEFEGARRSRQAQLGLRDL
                     KQEADGVICLPNQKVFKLIDQNTSVNEALKITNEVLAQGVRGIWRLLTQTGLINVDLN
                     DLCAVLRGRHEESSLATVEASGENRSHEVIEKLMTHPFLEAGQSLAEADTVLVSLAGG
                     PDMTMAEVNRVMEQINRQCENAHIIMGAGIQETCAGRLSVTLVASRQNGNVREREERQ
                     PARVPLQKDSTEAGHEQVHEMLNPTVAPRPASRYVPPAPSLTQQETHRLLTTQQGGGS
                     RSRKKNSSMRQSTLALEIVSKGRFEKSEPTIIHGEDLDVPTYIRRGVALN"
     gene            complement(6393..>7115)
                     /gene="ftsA"
                     /locus_tag="VG754_10935"
     CDS             complement(6393..>7115)
                     /gene="ftsA"
                     /locus_tag="VG754_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWF19777.1"
                     /translation="LQNPIRVVKGLQLEVEAIVFNGLASSLALLTNEQKELGALVIDI
                     GGGTTNYAVYADGIIKHTGVLAVGGDHVSNDLAYGLKVPLGRAEQLKIEHGSALLDDS
                     VKGQTITISNELGLPLKTINLEHLRRVMSLRLEELLQLIEQDIARAGLLDYLRAGVFI
                     CGGGARVPQILKLAERVFQMPVSLGKTNSISGIRSALDQPEFATAIGLVKFGSFQQKK
                     RASGGFFGDGFKKTIGEIFNRK"
BASE COUNT         1575 a         2268 c         1751 g         1521 t
ORIGIN      
        1 atcactttcg agcgaagcga ggcgagcttg cgagcatcca gcttcaaata caaaccatct
       61 aaaaatcacc ccctctactg caccctcacc tcacctttac ccttcacaat ctctccaatc
      121 acccacgctt tttgtttttg cgcgcggatg aatttcaaca ccgcatccac tttctccccg
      181 ctgacgatga tcgtcatgcc gatgcccata ttgaacacct ggtacaattc cgcttccggc
      241 actccgcctt tcgcctggat catctggaaa atcggcaaca cgtcccacgt acccttgcgg
      301 atgatgacat cgcaattctt cggcaagacg cgcggaatgt tgtccacaaa accgccgccc
      361 gtgatatgcg caagcccctt gatgatttga aatttcaaat ttgaaccttg agattttgcg
      421 aaggcggcat tgaacttctt gagcaaggtt tgaatcaacg gcccgtaact cacgtgcact
      481 ttcaacaatt catccccgat cgtattgccc aattccgcca cgcggctttt cggcttcaac
      541 ttcatctgtt cgaaaaaaat ctttcgcgcc agggaataac cattcgtgtg caatccgctc
      601 gatgccaaac caatcaccgc atcgcccggc cggatgcctt tgccgtccag catccggttc
      661 ttttccacca caccgacgat ggtaccgctg acatcgtatt cgcccggttg atagaagccc
      721 ggcatttgcg ccgtctcgcc gccgatgagc gcgcaattat ttttcgcgca accgcgcgca
      781 aaaccctcga tcaattgcgt gaacacatgc ggcttgagct tgccggtgcc gatgtaatcg
      841 agaaaaaaaa gcggctccgc gcccagcacc gcgatgtcat ccacgcaatg gttcacgagg
      901 tcttcgccga tggtgtcgtg cttgtccatc gcgaaggcca gcttgagctt cgtgccgacg
      961 ccgtctgtgc tcgaaaccaa aacgggctcc cgatattttt tgacatcgag cgcgaacaag
     1021 ccgccgaaac cgccgacctt gccgagcact tcgcggcgat gcgttgcggc cagcaattgc
     1081 gggagcgtcg ccttgacgcg atttcccaag tcgatatcga cgcccgcgcg ggcataagct
     1141 ttctttttca tcaggagcac tacaaaatgg gttcatcctc cttcggtcaa tacaaaaaca
     1201 atccggccgt gaacttgtcg tttagaccac gccatctcct cccgtcttcg gtccttcctc
     1261 cgccaatttc agccgcagct tcgccacgcg cagtccctcc atttcctcca cttgcaaagc
     1321 gtaattgccg agggacaaaa catcgcccgg tttgggaaaa ccgcccaacc gatgcgtcac
     1381 ccaaccgctc acggtggtga tgccttcttc ctgcaacggc tcgcgaacca attccgccaa
     1441 ttcgtgcaac ggcagcgtcc ccgccaattc ccaagtctcc gcgccggtct tcaccaaaag
     1501 cggtttttcc tggtcgaact cgtcctggat ctgtcccacc aattcctcaa gaatgttttc
     1561 cagcgtcacc attccgaccg ttccgccgta ttcgtccacg acgatcgcga gatgcaattt
     1621 tcgttcgaga aaaagttgca gcaacttttc aagccgtgcc gtttccggca cgtaaatcaa
     1681 ttttcgcgcg accgtaatca ggtcagctcc gctccgcgcc ttcaatcgcg cggcaaacaa
     1741 atccttgaaa tgcaccacgc ccagcgtctt gtccagattg ccgcgttcgc acaacggaaa
     1801 ccgtgaataa cgcgtcttct ccgccacgtc gaggcattcg gcaatacttg cctcggtgtc
     1861 gaggcagacg atttcattgc gcgggcgcat cacttcgcgc gcgattcgcc ggcgcaaatc
     1921 gagcgcattc aaaacgatgt cgcggcctaa tcgcgtcgcg ccggaatacc ggtccgaaac
     1981 ggcaaacaac aaccgcaatt cctcttccga atgcgaacgt tgcgcctcgg aagcgggttc
     2041 gatgccgatc tgtttcaaca gccacatcgc cgagcggttc aggagccaga cgaacggaaa
     2101 cgtgatgcgc gcgaaccacg acagcggccg cgccgtccac aacgccaccg ggaccgtctt
     2161 acgtatcgca atcgccttcg gcgcgagttc gccgatgact atcagcaaaa aagagttcac
     2221 gaaaaatccg gcgaggattg cgacggtgtg ctgcacggtt tgcgattcga cattcagcca
     2281 gtggaagact ggcgcgagca acgacaagaa caccggctcg accaaaattc caaggcccaa
     2341 gctcgcgagg gtgattccaa actgcgtcgc gctgatggcc gcttccaggt tttgcgccaa
     2401 acggcggaca atccttgcgc ggcgattgcc gccctgcacg agcgtttcaa tctgcgtgtc
     2461 gcggattttc accagcgcca gttccgccgc gacgaaaaat ccgttcagca gcaccagcgc
     2521 cgcaacgccg agcaatttca aaatattggc catcgccggg tccattttac aaacgcacct
     2581 ctccaaagat aaccttcaag atgtcctgca acgtcacaat ccccgcttcg cgctggtcat
     2641 gcccaagcac gatggcgagc cgttgtccgc tgcgctgcat ccgcttcaac gcctcttcga
     2701 gccgcatgtt ttcctccatg tacaaagcgg gccggacgta atcaccggca gttttctccg
     2761 gatccaattc ggcttggtac acaaccgccc gcaagctcac gaggccgaca atcttgcgct
     2821 cgtttccttc gccgcgccag accgggaagc gcgacagcct cctctcctgc gatattttca
     2881 atgcgtcacc catgcgggtc tgcatcgtga gggtcgcgac cttgtccatc gggatggcga
     2941 tgtcccgcac cgtgcggttc tgcaaatcga gcacgcgatt gatcatctgc cgctcctcgg
     3001 aggtgagtcc ctgcgcggat tcctgcatca ccaggcgcat ctcttcgcgg ctgccgaaca
     3061 gatgacccat gaacgcccgc ccgcccgtcc atttcaaaaa gcgttgcgac actgtggtga
     3121 tcaatttcac cagtggcgac aacacgaggt gaatcaatcg aaacggcctc gcaatcgcga
     3181 gcgtcagccg gttcgggaac atgcgaaaga gcatctttgg cagcaattcg aacaacgcat
     3241 aaaacaaaaa catcagtgcc agaaaactga tgagaaaaaa tcctggatac ggatcgagcc
     3301 atcggcgcaa caacaccacc atcagcccca cggcgatgaa attcgccagc atgtttccca
     3361 cgaggattgt ccaaagaaaa tcttccggcc gttccagata atcgagcaat gcgcgcgcat
     3421 tgggattgcc cgcgcgcaac atttgccgga tgcgcagccg gctcaatgcc agcacgcccg
     3481 cttccatccc cgaaagcagg aatgacatca ccacgcttcc cacaaacaaa agcgatatga
     3541 ggaaaatagg catcatgcaa tcatcgcctc aacgtctcca ccatcaattc gcgcacccgc
     3601 cgttcatccg ccgactgtgc ggtcaatttc agcccgcgga aagtgacggc atcacctact
     3661 gcgggaatcg tttcgtgcaa cgcaaccatc aatccgccca ttgtgtccac ttccgatact
     3721 tcgccgagcg caggatattc gcgccgaaaa tcttccaggc gcattgtccc gttgacgcgc
     3781 cagcggccca cacccaattt ctcgatcaca aaaccttccg gttcgccttc gccgcggatt
     3841 tcgccgacca tctcttccaa gatgtcctgc atcgtcacga tgcccgccgt gccgccaaat
     3901 tcatccagca caatcgccag cccgcgctgc tggcgttgaa ggcttttcag caattgcaac
     3961 aagttcatgc tctccggcac gaacgatgga aattcaatcg cgtccgccaa atccatttcc
     4021 gggttccaca acaacgcgcg cgcattcaac acgccgacga ttgtgtccag cgtttcgtca
     4081 taaagcggca aacgggtgtg cttaaatttg cgagccgccg cgatcatttc ttccaccggc
     4141 aaatcatctg aaatcgccgc catctgcgcg cgcggcttca tcacgtcttg ggcggtttgc
     4201 cggtcaagcc cgatgatttg caggataatt tccttttccg tgcccgccaa cgtgccttgt
     4261 tgaaaagcca gttcgagcag ttcctgatat tcctcgtcgg acatcgccac gcccggtttg
     4321 atcgattgcg gaaccagcgc gttgaggatg gcgttatcga gccgttgcgc aacgcggcgg
     4381 aacggcccgg tgaatttttg cagaaaaagc atcggacgcg caacgcgcaa cgcccataat
     4441 tccggcgagc gcacggccaa agtttttggc gcgacttcgc aaccgagcag aatcaaaacc
     4501 agcaacgccg gcagacaaac aaaccagagc aaaggccccc acggcgccgc ccagagcgtg
     4561 atggcgagta tggaagcgtt ggccaccgta ttaccgagca caatcgttgc aagaagatgt
     4621 tgcggctccg cgagcaatcg cgcgaccaaa ccgcccgcgt tcggcgaacg ttccgccaat
     4681 tgccgcaact gccatttgcc tagggagaac aatgccgatt cggcgagcgc gaagaaaaag
     4741 ctcatcccgg cgaaaaccag caatccaacc ccggccaaat aaggcatcag cgccatttcc
     4801 aaaatattag cctgctatgg gaaaggcaaa agggaaaaga aaatcatttc gttgacggtg
     4861 acaatcgtca gcttacaagc tgctgaaaaa ggcggatgcg gacattgagc atgataatct
     4921 tacccatttg caatgatgat gtcgtatttg taagagtcga tgtgaggagg ctctttttgg
     4981 atttcaaatt tgaaatttgg aatttcaaat caaaggaggg cctcctcacg tcggctccta
     5041 caaaaatggg acggattaat tcaatgccac gccgcggcgg atatacgtcg gaacatccaa
     5101 atcttcgccg tgaatgatgg tcggttcgct tttttcaaag cggcccttgg acactatctc
     5161 caatgccaat gtactttggc gcatcgacga attctttttg cgtgaacgcg aaccaccgcc
     5221 ttgttgcgtg gtcaacaaac gatgggtctc ttgctgcgtc aatgagggtg cgggcgggac
     5281 ataacgcgat gccggacggg gcgcgaccgt ggggttcagc atctcatgga cctgttcgtg
     5341 accggcttcg gtcgagtctt tttgcaaagg cacccgcgcc ggttgacgct cttcgcgctc
     5401 gcgaacgttt ccgttttgcc gcgatgccac cagtgtcacc gacaaccgtc ccgcgcacgt
     5461 ttcctggatg cccgcgccca tgatgatgtg cgcgttctcg cattggcggt taatctgttc
     5521 catgacgcgg ttcacttctg ccatggtcat gtccggtccg ccagccaaac tcaccagcac
     5581 ggtgtccgcc tctgccaacg attgcccggc ttccaaaaaa gggtgcgtca tcaatttctc
     5641 gataacttcg tgcgagcggt tttcgcccga ggcttcgacg gtggcgagcg aactttcctc
     5701 atgccgtccg cgcaacacgg cgcaaaggtc gtttaaatcc acattgatca atcccgtttg
     5761 tgtgagcaac cgccagatgc cgcgcacgcc ttgcgcgagc acttcattgg tgatcttcag
     5821 cgcttcgttg acgcttgtgt tctggtcaat caatttgaaa accttttggt tcggaaggca
     5881 gatcacaccg tccgcctcct gcttcaaatc gcgcaggccc aattgcgcct gccgcgaacg
     5941 gcgcgcgcct tcaaattcaa acggcaacgt gacgatcccc agcaccaacg cgccggtttc
     6001 ctttgccaac cgcgccacaa cgggtcccgc gcccgtgccg gtcccgccgc ccaaaccggc
     6061 gaccacgcag acaatgtcag ccccggcgca aagcgagcgg attttctcga ggtcttcctc
     6121 cgccgcggcg cggcccattt ccgggtcgcc gccggtgccg aggccgcgcg tcaattttgc
     6181 cccgagcgtc atccgttcca ctgtgctcaa ttgcgacaac gccttcgagt ccgtgttgat
     6241 ggccatcatg ctgacgccgt caaaatcctg ccgcgccatg taatccaccg catttccgcc
     6301 tccgccgccc acgccgaaaa ctttcagcgt caattttttc gcgacgaatc ccgtttcagc
     6361 cagttggttg gtttccgatt gcatagattt ttttatttgc ggttgaaaat ctcgccgatg
     6421 gttttcttga atccgtcgcc aaaaaatccg ccgctggcgc gtttcttttg ctggaacgag
     6481 ccgaacttca ccaggccgat ggcggttgcg aattccggct ggtcaagcgc cgacctgatg
     6541 ccgctgatgg aattcgtctt gcccaacgaa accggcatct gaaacacccg ttccgccagt
     6601 ttgaggattt gcggcacgcg cgcgccgccg ccgcagatga acacgcccgc ccgcagataa
     6661 tcgagcagtc ccgcccgcgc gatgtcctgt tcaatcaatt gcaaaagctc ctccaaccgc
     6721 agcgacatca cccgccgcaa atgttcgaga ttgatggtct tgagcggcag gccgagttcg
     6781 ttcgagatgg tgatggtctg gcctttcacg ctgtcgtcga gcaatgccga gccgtgttca
     6841 atcttcagtt gctccgcgcg accgagcggc actttcaatc cgtaggccaa atcattcgaa
     6901 acatgatcgc cgccgaccgc gagaacgccc gtatgcttga tgatgccgtc ggcatacacc
     6961 gcgtaatttg tcgtcccacc gccgatgtca atcaccagcg cgcccaattc cttctgctcg
     7021 ttcgtgagca acgcgagcga cgacgccaag ccgttgaaaa caatcgcctc aacttccaat
     7081 tgcaaaccct tcaccacgcg gatcggattt tgcag
//
LOCUS       DASZMM010000454         4132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1836791, whole genome shotgun sequence.
ACCESSION   DASZMM010000454 DASZMM010000000
VERSION     DASZMM010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836791"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /gene="dacB"
                     /locus_tag="VG754_10940"
     CDS             <1..706
                     /gene="dacB"
                     /locus_tag="VG754_10940"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HWF19778.1"
                     /translation="LFAAVFKDALAQNGITVAGRARPMHYMDREVAPFNPAEYVELGS
                     VQSLPFRDIIRAVQKPSQNLYTDLVLEHIGAGMLDGKSEASGEQAGIRELGRFLQKAG
                     IPRGQTIFEEGSGLSRDNLTSPNATVTLLTYMSRHKDAEVYINALPIAGVDGTLRRRM
                     KGTPAQGNVRAKTGTLRWASSMSGYVTSAAHERLVFSMMLNRYSGGSKTEDLDAIAEL
                     LAGFNGHTPVEVSGGH"
     gene            complement(703..1050)
                     /locus_tag="VG754_10945"
     CDS             complement(703..1050)
                     /locus_tag="VG754_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWF19779.1"
                     /translation="MEWCKPLRSAPYYAMDSDGPIQLPIDGVLDLHTFQPREIKELVT
                     DYLAACQEHGILQVRIIHGKGIGNLKRSVHALLARHPEVISYSLDHPQFGGWGATLVT
                     LRQKPGNDASAKT"
     gene            complement(1186..2733)
                     /locus_tag="VG754_10950"
     CDS             complement(1186..2733)
                     /locus_tag="VG754_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19780.1"
                     /translation="MPPKLHKALLSTVMVVLLVGVSFIQRSLVKDRDALGLTRYTELK
                     GAPPVLALTTVALGGFRGLISNILWIRASDLQENDKFFEMVQLADWITKLEPHFTQVW
                     LVQSWNMAYNISVKFKDPPDRWRWVKRGIELLRDQGMQYNPDDVLMYRELAWFFQHKM
                     GQNMDDAHLYYKQMWANEMHSVFGFDRPNWDELIHPQTEDQKKRADLLIQKYKMDPQF
                     MKEVDDRYGPLEWRLPETHAIYWAALGLKKAKENPRHIDPKDLITLRRVIYQSMQLAF
                     QRGRLIANQTGKFFEFGPNLEMIPNASAAYEREMEADPPNRDHIETAHRNFLRQAVFF
                     LYTYNRQADAVAWFKYLAEKYPNKPILDGDPNSFPRNISLDDYAIGCINEELKDAGMD
                     KTKGVLEGLERTAYLALIDDEDDRFIGLNRLCEKIWNHYQEKVGGSAQTQGRLPLPPL
                     AHIKENVLNNLLEQLQPQMAAVLRTKLNLPSTGTIHKAAQAPTLSVLAPDPDSTNAPP
                     STNAVPK"
     gene            complement(2736..>4132)
                     /locus_tag="VG754_10955"
     CDS             complement(2736..>4132)
                     /locus_tag="VG754_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19781.1"
                     /translation="IMQRLFAIITLTWKAAFRYRLFWVITSLLLAAVIFLPLLIKDDG
                     TAAGFAQILLTYTLIAVTGLLGICTLWLSCGTLARDVEDCQIQMVAVKPIARWQIWLG
                     KWLGIVLLNAALLAVAGASIYCLLEFRARKLPPTQLSKLQNEVLIARGSAREGNLNDV
                     IEKETERRMKEQKDKGKLADLDARTIQQQITEGLKAELQVVPPGGARIWRIHLGKAAA
                     EELKDQPLFVRTKFVTGDPATTGNTFIGDWYAGVPQKTRVWHELMPSLASDTFHEFRI
                     DPNLYDENGDIVIAFRNVNDIALLFTFEDGMELLYREGGFGLNFIRGLAVILCWMALL
                     AAIGLATASFMSFPVAAFVSMALLAITFSSGTLSTVVSDGTIMGWDSEKSAKGHSPLD
                     NIAIPVFRVMLDTIQLVQQFSPIDSLSTGRSITWAELAKAVGQIVLLMGGVLGLIGIF
                     IFNRRELATAQGNH"
BASE COUNT          862 a         1138 c         1236 g          896 t
ORIGIN      
        1 cttgttcgcg gcggttttca aggacgccct tgcgcaaaat ggcatcactg tggccggacg
       61 cgcgcgcccg atgcattaca tggaccgcga agtcgccccg ttcaatccgg ctgaatacgt
      121 cgaactcggt tccgtgcaat cgctgccgtt tcgcgacatc attcgcgccg tgcaaaagcc
      181 gtcgcaaaat ctttatacgg atttggtgct ggagcatatc ggcgcgggaa tgctggatgg
      241 aaaatcggaa gcgagcggcg aacaagcggg catccgcgaa ttgggccggt ttttgcagaa
      301 ggccggcata ccgcgcggcc agactatttt tgaggaaggc tccggccttt cgcgcgataa
      361 tctcacttcg ccgaatgcga ccgtcacgtt gctcacatac atgagccgtc acaaggacgc
      421 ggaggtttac atcaacgcgt tgcccatcgc cggtgtggac ggcacgttgc ggcgtcgcat
      481 gaaaggcacg cccgcgcaag gcaatgtgcg cgccaagacc ggcacgttgc gctgggcgag
      541 cagcatgtcg ggatacgtca ccagcgccgc gcacgagcgc ctggtttttt cgatgatgct
      601 caaccgctat tcgggtggtt caaaaaccga ggatcttgat gccatcgccg aattgctggc
      661 cggtttcaac gggcatacgc cggtggaagt gagcggcggc cattaagttt ttgcggacgc
      721 gtcgttgccg ggtttttggc gcaaggtgac caaggtcgcg ccccaaccgc cgaattgcgg
      781 atggtcgagc gaataggaaa tcacttcggg atgccgggca agcagcgcat ggacgctgcg
      841 cttgagattg ccaatgccct tgccgtgaat gatgcgtacc tgaagaatgc cgtgttcctg
      901 gcaggcggcg aggtaatccg tcaccaactc cttgatttca cgcggctgaa acgtgtgcaa
      961 atccagcacg ccatcaatcg gcaactgaat cggtccgtcc gagtccatgg cgtaatatgg
     1021 cgcgctgcgc aatggtttgc accattccat cgcgggaaat gtccgaacag cggcaaatgg
     1081 tgaaagccct ctccccgggg gagagggttt gggtgagggc gagcgtcatc caaaccgtac
     1141 ctgtacattc tcaacaacag tgtcaagatg cacccaatcc caacgctatt tcggcacggc
     1201 attggtggac ggcggtgcgt tggtagaatc ggggtcggga gcaaggacgg acaacgtcgg
     1261 ggcttgagcc gctttgtgga tcgtgccggt ggaaggcaga ttgagcttgg tgcgaagaac
     1321 ggcagccatc tggggctgga gctgttccaa aagattgttc aacacgtttt ccttgatgtg
     1381 ggccaacggc ggcaaaggca gccgtccctg ggtttgcgcg ctgccgccga ctttctcctg
     1441 ataatggttc caaatttttt cgcaaagccg gttgagacca atgaaccggt cgtcctcatc
     1501 gtcaatcaac gccagatatg ccgtgcgttc caatccttcc agcacgccct tggtcttgtc
     1561 cattccggcg tccttgagtt cctcgttgat gcaaccgatg gcataatcat cgaggctgat
     1621 gttacgtgga aacgagttcg gatcgccgtc gaggatgggt ttgttgggat atttttcggc
     1681 gagatatttg aaccaggcga cggcgtcagc ctggcggttg taggtgtaaa ggaaaaacac
     1741 cgcctgacgc aaaaaattgc ggtgcgcagt ctcgatgtgg tcgcgattgg gcgggtctgc
     1801 ttccatctcg cgttcgtaag cggcgctggc gttgggaatc atttcgaggt tcggaccaaa
     1861 ttcgaaaaat ttgccggtct gattggcgat aagccgtccg cgctggaagg ccagttgcat
     1921 ggattgatag atgacacggc gcagtgtgat gaggtccttg ggatcgatgt ggcgcggatt
     1981 ttccttcgct tttttcaaac ccaatgcggc ccaataaatg gcgtgggttt ccggcaaacg
     2041 ccattcgagc ggcccgtaac ggtcgtccac ttccttcatg aactgcgggt ccatcttgta
     2101 tttttggatg agcaggtcgg cgcgcttttt ttggtcttcg gtctggggat ggatcaattc
     2161 gtcccaattc ggccggtcga aaccgaacac gctgtgcatt tcatttgccc acatctgttt
     2221 gtaatagagg tgggcgtcgt ccatgttctg ccccatcttg tgctggaaaa accaggccag
     2281 ctcgcgatac atgaggacat cgtcgggatt gtattgcatc ccctggtcgc gaaggagttc
     2341 gatgccgcgc ttgacccagc gccagcggtc gggcgggtcc ttgaacttga cggaaatatt
     2401 gtaggccatg ttccaggatt ggacgagcca gacttgcgtg aaatgcggct cgagcttggt
     2461 gatccaatcg gcgagctgga ccatctcgaa aaatttgtcg ttctcctgaa ggtcgcttgc
     2521 gcgtatccac aggatgttgg agatgaggcc gcgaaaaccg cccagcgcga cagtggtcag
     2581 cgccaacacg ggcggcgcgc ctttcaattc ggtgtaacgc gtcaatccga gcgcatcacg
     2641 gtccttcaca aggctccgtt ggatgaacga gacgcccacc agcaacacca ccatcacggt
     2701 ggacaggagc gctttgtgca gtttgggagg catcgtcaat ggttcccctg cgccgtggca
     2761 agttcgcggc ggttgaagat gaaaatgccg atcaacccaa gcaccccgcc catgagcagg
     2821 acgatttgcc cgacggcttt cgcgagttcc gcccaggtga tgctgcgccc ggtgctcaat
     2881 gaatcaatgg gcgaaaattg ttgcacaagt tgtatcgtat cgagcatgac gcgaaacacc
     2941 gggatggcga tgttgtccag cggcgaatgc cctttcgcgc ttttctcaga gtcccagccc
     3001 atgatggtgc cgtccgaaac gacggttgag agcgtgccgc tggaaaatgt gatggcaagc
     3061 agcgccatcg aaacgaatgc cgcgacggga aaggacatga aacttgccgt tgccagcccg
     3121 atcgcggcga gcaacgccat ccagcataaa atgaccgcca gcccgcggat gaagttcagc
     3181 ccgaacccgc cctcgcgata cagcaactcc attccgtcct cgaacgtgaa cagcagcgcg
     3241 atgtcgttca cgttgcggaa cgcgatgacg atgtcgccat tttcatcgta aagatttgga
     3301 tcgatcctaa attcatggaa cgtgtcggac gccaggctgg gcatcagttc atgccagacc
     3361 ctggtttttt gcggcacccc ggcgtaccaa tcgccgatga aagtgttgcc ggtggtcgcc
     3421 gggtcgccgg tgacaaattt tgtgcgcacg aaaagcggct ggtccttgag ttcttccgcg
     3481 gcagccttgc cgaggtggat tctccagatg cgcgcgccgc cgggcgggac gacttgcaat
     3541 tccgccttca gcccctcggt gatctgctgc tgaatcgtcc gcgcatccaa atccgccaat
     3601 ttacctttgt ccttctgctc cttcatgcgc cgctcggttt ccttttcgat cacgtcgttg
     3661 aggttgcctt cccgcgccga accgcgggcg atgagcactt cgttctgcaa tttgctgagt
     3721 tgcgtgggag gaagtttgcg ggcgcgaaat tcaagcaggc aataaatgct ggcccctgcg
     3781 accgcaagca atgcggcatt gagcaaaacg atgccgagcc atttgcccag ccagatttgc
     3841 cagcgcgcga tgggtttgac ggcgaccatc tgtatctggc aatcctcaac gtcccgcgcc
     3901 agcgtgccgc aggaaagcca gagcgtgcaa atgccaagca aaccggtcac cgctatgagc
     3961 gtgtaggtga ggaggatttg cgcgaaaccc gccgcggtcc cgtcatcttt gatcaggagc
     4021 ggcaggaata tcaccgccgc cagcaacagc gacgtgatga cccaaaaaag ccggtaacga
     4081 aaagcggcct tccaagtcag cgtgatgatg gcgaaaagtc gttgcattat gc
//
LOCUS       DASZMM010000455         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1001571, whole genome shotgun sequence.
ACCESSION   DASZMM010000455 DASZMM010000000
VERSION     DASZMM010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001571"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VG754_10960"
     CDS             <1..1233
                     /locus_tag="VG754_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWF19782.1"
                     /translation="TLDGRPIEQGEIAGPLIENHDNFQNAQAFLEGGGRRGLQEQILL
                     SGTWNLNPWFVQVEQVPMLEIPIGHVGVVISFVGEAHEDVSGVEFKHGDLVNMGHKGV
                     WVTPLQPGKHPVNTRVMKVERVPTTNIVLNWATRTEAHNYDAKLSSITVRSRDGFAFN
                     LDVSQIIHVGALDAPKVISRVGSMQNLVDHVLQPIVGNYFRNSAQNYTVLDFLSARSE
                     RQTEAAQHIRVALAAYDVQAIDTLIGDINPPAALMETQTDRKIAEEQKKTYEVQEAAQ
                     KQRQQLVRETAVADIQQQVVSAEQGVNIAELHAGAQVKHAGGEAEATRLRALGEAEAI
                     RATGNAKAEAYRAGVEAIGAQGYTALQLMQVVGERNVRIVPDVSVSGANGSTGLVDGM
                     LGMVLRDQSAKKAAGSAA"
     gene            1797..>3653
                     /locus_tag="VG754_10965"
     CDS             1797..>3653
                     /locus_tag="VG754_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19783.1"
                     /translation="MIFPWTKKGTASQPLKSGAQERGNGVQRSATPQQPKSDQSCTAS
                     VHPSSAPRITTPVRPMVRFPNNPFCRIEDVIVEGLDFLAETPPNLPAATKCLANAEHA
                     AASKVLPERLACRMRFFTKSLERIRKDLLRGGVGREFKEVQSLPELKPPCVDRAAEGL
                     QSAALLKEAPALQTEMETLRHKVLVLEKKLERRRAARRWTLPPPALHIPRAGLQTAAV
                     LIAGVGILVTSCLWVGRPGEIGALQVKVIPAGAELFSNGVAMGNQNYPLRLTGFAGDK
                     MELEARLAGYSRVKRTLSLPAAAKNADETIELQPLPATLVVQSEPAAQQAWVRLEGEQ
                     PSAANNIADGIPVTAGKPMEISLTLPDYAPVSRTLEALKPGERRVLDLGALSIVPAAL
                     RLKVEPDDANFFVAYPGRDPIAFGNSNYLTGLTPKTPFKLIAERRGYASLTNTLNLKP
                     GQLCDLDLGRMQPSPASLMVLALPRPFEMQVEWDGQSHNFGVAGEATGLPPGKPLVVT
                     VAAEGCETWSTNLVLEPAEARTLSLGRLPFAQGYLLVNSTPGGGVVSYRVDGGMENVI
                     GVTDTIPSLPLGHQVSVTLGKPGYISVTKSCLFQHSGEMLEFGTLKPSLTFLT"
BASE COUNT          794 a         1045 c         1109 g          705 t
ORIGIN      
        1 acgctggacg gacggccgat tgagcagggt gaaatcgccg gaccgctcat cgagaatcac
       61 gacaacttcc agaacgcgca ggcgttcttg gaaggcggcg gacggcgcgg attgcaggag
      121 caaatcctgt tgtccggcac gtggaacctg aatccctggt tcgtccaggt ggaacaggtg
      181 ccgatgctgg aaattcccat cgggcacgtc ggtgtggtca tctcgttcgt cggcgaggcg
      241 cacgaggacg tcagcggcgt ggaattcaaa catggcgatc tcgtcaacat ggggcacaaa
      301 ggcgtttggg tcacgccgct gcaaccgggc aaacatccgg tgaacacgcg cgtgatgaaa
      361 gtcgaacgag tgccgacgac gaacatcgtc ttgaattggg ccacgcgcac cgaagcgcac
      421 aattatgatg cgaagttgtc ttccatcacg gtgcgttcgc gcgatgggtt cgcgttcaac
      481 ctggacgttt cgcaaatcat ccatgtcggc gcgctcgatg cgcccaaggt gatttcgcgc
      541 gtcggttcca tgcaaaacct tgtggaccat gttttgcagc cgattgtggg aaattatttc
      601 cgcaattcgg cgcagaatta cacagtgctc gacttcctca gcgcgcgcag cgaacgccag
      661 acggaagcgg cccagcacat ccgcgtcgct ctggcggcgt acgacgtgca ggccatcgac
      721 acgttgatcg gcgacatcaa tccgcccgcg gcattgatgg aaacgcagac ggaccgcaaa
      781 atcgcggaag aacagaagaa gacctacgag gtgcaggaag ccgcgcaaaa gcagcgccag
      841 caactcgtgc gcgaaactgc cgttgcggac atccagcaac aggtcgtcag cgccgaacaa
      901 ggcgtgaaca tcgccgagtt gcacgcgggc gcgcaggtca agcacgccgg cggcgaagcg
      961 gaagcgacgc gtttgcgcgc gttgggcgaa gcggaagcaa tccgcgcgac cggcaatgcc
     1021 aaggccgaag cgtatcgcgc gggcgttgaa gccatcggcg cgcaaggcta cacggcgctg
     1081 caattgatgc aggtcgtcgg cgagcgcaac gtgcgcatcg tgcccgacgt ttccgtcagc
     1141 ggcgcgaacg ggagcaccgg cctggtggac ggcatgttgg gcatggttct ccgtgatcaa
     1201 tccgccaaga aagcggctgg cagcgcagcc tgagcttggg gatggggaaa caacaagcgg
     1261 cgttccggaa acgggatgcc gctttccttt gaaggaatcg gcaagaaata ttgccaatgc
     1321 agacttgaat tgtaattata gaaataacga gagagcgtat atttatgcgt gcgagtttca
     1381 acaggcggac tgccacaaag gtgagagacg ggagggtgca gcgaaaaaat cggcatttac
     1441 ccaccggact tctcggctgc gtgatccatc gcgatgaaac tgtccggctc agagcgagtc
     1501 attattggtt gtaaaatgtt tgatgcctgc cgcgacgaca ttttggcctc attcccaaaa
     1561 gttttgtcgc cggatgaatg gagcggagat tgcacgagca gatttacggc aaagcaaggc
     1621 cttattgagc ctggtgcgga cacagccaat tcccttctcc atcaagtgag gacttaacac
     1681 agtaatgtta cagacgtacc ggggctcgtt gccttggcag gtggattaga accccatggc
     1741 ggcaattgca aaatacggta actttgtggc gatcgtaact ggcagcaccg tttcaaatga
     1801 tttttccgtg gacaaaaaag ggcacggcaa gccagccgct gaaatcaggc gcgcaggaac
     1861 gcgggaatgg cgtccaacgc tccgcaacgc cacagcagcc gaaatctgat caatcttgca
     1921 ctgcttcggt ccatccgtcg agcgctcccc ggataaccac gcccgtgcgg ccgatggtgc
     1981 ggttccccaa caatccattc tgccggatcg aagatgttat cgtcgagggc ctggattttc
     2041 ttgcggaaac gccgcccaat cttcctgctg ccacgaagtg cctggcaaat gccgagcatg
     2101 ctgccgcttc caaagtattg cccgagcggc tggcttgccg gatgcgtttt ttcacaaagt
     2161 cccttgagcg cattcgcaaa gatttgctgc gtggtggcgt cggccgggaa ttcaaagaag
     2221 tccaatcgtt gcccgaattg aaaccgcctt gtgtggaccg cgccgccgaa ggattgcagt
     2281 ccgccgcgct gctcaaggaa gcgccggctc tccaaaccga aatggaaacc ttgcggcaca
     2341 aggtgctcgt gcttgaaaaa aagttggagc gccgccgtgc cgcgcggaga tggactttgc
     2401 cgccgccggc cttgcatata ccgcgcgccg gacttcagac cgcggcggtg ttgattgccg
     2461 gcgtcggcat tttggtcacg tcctgcctgt gggtggggcg tcccggcgag atcggcgctt
     2521 tgcaggtgaa ggtcattccc gccggcgcgg aattgttttc caacggcgtt gcgatgggca
     2581 accaaaatta tccgttgcgc ctgaccggtt tcgctggcga caaaatggaa ctggaagcgc
     2641 gccttgccgg ctattcacgg gtgaaacgaa ccctttcgct tcccgccgct gccaaaaatg
     2701 cggacgaaac catcgagttg caaccgttgc cggcaacttt ggtcgtgcaa agcgagccag
     2761 ccgcgcaaca agcatgggtg cggctcgaag gagaacaacc gtcggcagca aacaacatcg
     2821 cagacggtat tccagtgaca gccggcaaac cgatggaaat ctctttgacg ctgccggact
     2881 atgcgccagt cagccggaca ttggaggcat tgaagcccgg cgaacggcgc gtgctggatc
     2941 tcggcgcgct ttccatcgtg cccgccgcgt tgcggttgaa agtggaacct gatgacgcca
     3001 atttttttgt cgcatatccg ggacgcgatc ccatcgcttt cggcaacagc aattacctca
     3061 ccggactcac gccaaagacg ccgttcaaac tcatcgccga gcgccggggt tacgcgtcgc
     3121 tgacgaacac gctcaacctc aagcccggcc agctttgtga tcttgatctt ggtcggatgc
     3181 agccgtcgcc cgcgtcgctg atggtgctgg ccttgccgcg gccattcgag atgcaggtcg
     3241 aatgggacgg gcaatcgcac aatttcggcg ttgcgggaga agcgaccgga ttgccgccgg
     3301 gaaaaccgct cgttgtgacc gtcgccgctg aaggatgcga aacgtggtcc accaacctcg
     3361 tgcttgaacc ggcggaagcg cggacgcttt cgctcggtcg gctgcccttc gcgcaaggtt
     3421 atctgctcgt gaattccaca cccggcggcg gcgtggtcag ctaccgcgtg gatggcggga
     3481 tggaaaatgt catcggcgtg accgacacca ttccgagctt gcccttgggc catcaagttt
     3541 ccgtgactct cggcaagccc ggttacattt ccgtcaccaa gagttgcttg ttccaacatt
     3601 ccggggaaat gctggaattc ggcacgctca agccttcgct cacatttctc acc
//
LOCUS       DASZMM010000456         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_763688, whole genome shotgun sequence.
ACCESSION   DASZMM010000456 DASZMM010000000
VERSION     DASZMM010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763688"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..527
                     /locus_tag="VG754_10970"
     CDS             213..527
                     /locus_tag="VG754_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19784.1"
                     /translation="MNATGNGQSFPGSPTNRGSARMRSYARHIRKLLRLLLRAQPRSI
                     LVSYPELIFKFQISKFEVHTISDLKFQISNLKSADARPKKQFHIFLAKFHIVTIIVTV
                     WQ"
     gene            524..937
                     /locus_tag="VG754_10975"
     CDS             524..937
                     /locus_tag="VG754_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWF19785.1"
                     /translation="MNTQFSIAVHIMAGLACGCGEDITSGDLAMSVNTSPSFVRRILA
                     KLSKAGLVETATGKTGASWLARDPKKISLRDIYKAVDAPKVFSIHSYKEQKACMVSCH
                     IKAALENALGKTQKAMEKTLADISLAYIVSDVKKR"
     gene            1033..1791
                     /locus_tag="VG754_10980"
     CDS             1033..1791
                     /locus_tag="VG754_10980"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWF19786.1"
                     /translation="MSTNKKFTGKVAVVTGASKGIGASIAKHLATEGATVVVNYASSK
                     SGADKVVGEITGAGGKAIAVQGDVAKKADIERLFAETKKAYGKVDILVNNAGIYDFAP
                     LEQITEEHFHKQFNLNVLGLLLTTQEALKLFPVTGGNVVNISSLVSTLAPPNASVYSA
                     TKGAVDTITISLAKELGARKIRVNAVRPGLVETEGSVTSGFTQGDFHDEYVKNAPLGR
                     IGQPQDIAAAVAYLASDDSTWVTGETLLVSGGHH"
     gene            1824..2282
                     /locus_tag="VG754_10985"
     CDS             1824..2282
                     /locus_tag="VG754_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HWF19787.1"
                     /translation="MSATFFPDLTQRPPRSPRVRLGGYVFLPRIFDKIRASIAGKIGE
                     YRFGLKSMDRHFFNFVGLDPDALKNEIAKGGGDLELLTWVQTNAKHQRQPWEIAAWSE
                     YHLRRGPDSDTETLTDFADEVKKFNPAREDINTWFDLLDLDDYCTFGGKA"
     gene            2324..2551
                     /locus_tag="VG754_10990"
     CDS             2324..2551
                     /locus_tag="VG754_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWF19788.1"
                     /translation="MKMTDKGEITIPAAMRRKHGLLPRSEVRLVDQPDGVLIVKAEKV
                     SRGKRVLTTLLRGGRVKGTTETWLRSTRGTR"
     gene            complement(2596..2931)
                     /locus_tag="VG754_10995"
     CDS             complement(2596..2931)
                     /locus_tag="VG754_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19789.1"
                     /translation="MKEQLNAGKKQPLLKTTKSAAVESAQYPPPTALVELAELESKRR
                     RVEEYAEVISKLRDDKGFTYRAIAEWLNKNGVPTDHNEVYRTHKEWCEGALKRYFERN
                     RQPKVPNQD"
     gene            3091..3180
                     /locus_tag="VG754_11000"
     tRNA            3091..3180
                     /locus_tag="VG754_11000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3125..3127,aa:Ser,seq:cga)
BASE COUNT          832 a          901 c          898 g          741 t
ORIGIN      
        1 catccaagct ccaaacaagt tttgatggac cgcggctgtg tccgcaagga ccagccgcag
       61 cagcttgtac ggctcgggcg catccgcgca cgacgccgga gcgcgggtcg gcgacccgca
      121 gcaatatggg aagtataagt gtatgaatga gtctggcatg tcctgttcct cgcgcgttgc
      181 tgcggctcat cgagccgcgc tccgtttcgc cggtgaacgc gacgggcaat ggccagtcct
      241 tcccaggatc gccaacaaat cggggcagtg cgcggatgcg ctcgtacgct cggcatattc
      301 gtaaattgct tcgactgctg cttcgcgcac agccgcgctc cattctggtg agttatccag
      361 aattgatttt taaattccaa atttcaaaat ttgaagtcca caccatttcc gatttgaaat
      421 tccaaatctc aaatttgaaa tccgcagacg cgcggccgaa aaaacaattt catatttttc
      481 ttgcaaaatt ccatattgtg actattatag taacagtatg gcagtgaaca cacagttctc
      541 gatcgcggtg cacatcatgg ccgggctggc ctgcggatgc ggcgaggaca tcacgtccgg
      601 cgacctggcg atgagtgtga acaccagccc gagctttgtg cgacgcatcc tggccaaatt
      661 gtccaaggcg ggcttggtgg aaaccgcgac cggcaagacc ggcgcgtcct ggcttgcgcg
      721 cgacccaaaa aagatttcgc tccgggacat ttacaaggcg gtggacgcgc ccaaggtctt
      781 ttccatccac agctacaagg agcaaaaagc ttgcatggtg agctgccaca ttaaggcggc
      841 actcgaaaac gcgctgggga agacccagaa agcgatggaa aagactctag ctgatatcag
      901 cctggcgtac attgtgtcgg acgttaagaa gaggtaagtt ttttgtgatt tattgttact
      961 atttaagtaa caatatatta acgcggttga aacagaaatc gaaacaacaa cgaaaagaag
     1021 ggacagaaca ccatgagcac aaacaagaaa ttcacgggca aggtcgcggt cgtcaccggg
     1081 gcatccaaag gcatcggcgc gtccattgca aaacatctgg cgacggaagg cgcgacggtc
     1141 gtggtcaatt atgcgtcgag caaatcgggc gcggacaagg ttgtcggcga aatcaccgga
     1201 gcgggcggaa aagcgattgc cgttcaaggc gacgtcgcga agaaggcgga catcgagcgg
     1261 ttgttcgccg aaaccaaaaa agcgtatggc aaggtggata ttctcgtgaa caatgcgggc
     1321 atttacgact ttgcgccgct cgaacagatc accgaggaac atttccacaa gcaattcaat
     1381 ttgaatgtgc ttggcctgtt gctcaccacg caggaagcat tgaaactttt cccggtcacc
     1441 ggcggcaatg tcgtcaacat cagttcgctg gtcagcacgc tggcgccgcc gaacgcgtcc
     1501 gtttatagcg caaccaaggg cgcggtggat accataacca tctcgcttgc aaaggaactt
     1561 ggcgcgcgca agattcgcgt caacgccgtc cgtcccggcc ttgtggaaac ggaaggatcg
     1621 gtcaccagcg gcttcacgca aggcgatttt cacgatgaat atgtgaagaa cgcgccgctc
     1681 gggcgcatcg ggcagccgca ggacatcgcc gcggcggtcg cttacttggc gtccgatgat
     1741 tcgacatggg tcaccggcga aacgctgctc gtttcgggcg gtcatcattg aacggaaact
     1801 gacgcacaat caatttattc atcatgagcg caaccttctt tcctgacctc acacaacgcc
     1861 cgccgcgcag cccgcgtgtg cggctgggcg gttatgtttt tctgcctcgc atcttcgaca
     1921 aaatccgcgc gtcaattgcc ggcaaaatcg gcgaatatcg tttcggcctt aaaagcatgg
     1981 accgccattt cttcaacttt gtcggcctcg accctgacgc gttgaaaaac gaaatcgcaa
     2041 aaggcggcgg cgatttggaa ctgttgacgt gggtccaaac gaatgcgaaa catcaacgtc
     2101 agccgtggga aatcgccgcg tggagcgaat atcatttgcg ccgcggaccg gacagcgaca
     2161 ccgagacgct cacggatttc gccgacgagg tgaaaaaatt caacccggcg cgcgaagaca
     2221 tcaacacctg gttcgacctg cttgacctcg acgactattg cacgttcggc ggcaaggcat
     2281 gagcggattg actttgctcc cctcttgagc caaagattac cgcatgaaga tgacggacaa
     2341 aggcgaaatc accattccgg ccgcaatgcg ccgcaaacac gggttgctgc cgcgaagtga
     2401 agtccggctg gttgaccagc cggacggagt gcttatcgtc aaagccgaaa aagtatcgcg
     2461 tggcaaacgg gtgttgacca ccttgttgcg cggtggacgc gtcaaaggca ccacggaaac
     2521 ctggctgcgc tcgacccgag ggaccagatg atccttgtgg acaccagcac cctcttggtc
     2581 agccatagga gacaatcaat cttggttagg taccttcggc tgtcggtttc gctcgaagta
     2641 tctctttagt gccccctcgc accattcttt gtgcgttcga tagacttcgt tatggtcggt
     2701 cggcactccg tttttattta accattctgc aatagcccgg tacgtaaatc ctttgtcatc
     2761 ccgcagttta gagattactt ccgcatattc ttcaacacgt cttcgctttg attcaagctc
     2821 cgctagttca accaaagcag tcggcggcgg atattgcgcc gattcgacgg ctgccgattt
     2881 agtagttttt aatagtggct gcttctttcc agcgtttagt tgttctttca taatattttt
     2941 caattaaaca taaacataag aacatgtcaa catacaaaca aacaaaacta gacgaacaaa
     3001 cttttggggg gtctaacctg aggcggactg ttcgcgcgac aaatgagctt gccctgaaat
     3061 tcgggccgtg cgaagtaata ggtcgcggtt ggaagggtgg ctgagtggtt aaaggcacct
     3121 gactcgaaat caggagtagg ggcaactcta ccgtgggttc gaatcccacc ccttccgcca
     3181 aatttaagat tcccagggcg agatcgtcag cgagggaggc gcggcagtaa tcaacgcccc
     3241 cgacgacaaa cgtttccgac accctccttc cgtccatgtc atattatcgt atgactattt
     3301 tctctggcga cttgtgttta aacttcgacg aaatcaaatc tctaacgtgc atgaaacaca
     3361 ttttagcaac ag
//
LOCUS       DASZMM010000457         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_492118, whole genome shotgun sequence.
ACCESSION   DASZMM010000457 DASZMM010000000
VERSION     DASZMM010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492118"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..>2272
                     /locus_tag="VG754_11005"
     CDS             218..>2272
                     /locus_tag="VG754_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWF19790.1"
                     /translation="MPTQDLQSLSGTFLLDDARLARESFLAAPWHERIPQDIYLNDIL
                     PYACANETREGWRKVLREKCLPLIADCKTPAEAAQRLNENLFKLTNVKYSTERKKADQ
                     SPSETMESGLASCTGLSILLIDACRSVGVPARLAGTPMWVNMRGNHTWVEVWDTDWHF
                     TGAAEPDAKGLDHTWFEHDASEAQKDNPQHAIYAASFKKTGLSFPLDWAPDIHWVSAV
                     NVTDRYTPKAAAPEQDKVRLMVKVLDHLAGQRVEARVIVSNIADGKIHFEGVSKGESV
                     DMNELLAFDVPRGATYDIRAEQFGQEAGREFIAGTNSQAVVVISLNDTTLFKFPSQAC
                     YVPPAVSHSLKPRDEEKLKAALVEFFAAPSKKQATWKFPRSMEKLLRENEPAVRRVAW
                     EAYVAAPIHETMKKDFDGHQARFETNLSPFTVRTVGERPAKGWALFIAMHGGGGVEKE
                     VNDSQWKIMQHYYKDHPEAGGYLYVALRAPNDTWNGFYDDYVYPLVANLVREFLLYGD
                     VDPNKVFIMGYSHGGYGAFAIGPKEPDLFAAIHASAAAPTDGETTGVTLRNTIFTCMV
                     GENDTMYGRIERDRKFKDAMAQLRAKDGTFYPVTVQIIPGNGHTGLPDRDKIKEMYPA
                     VRNPVPHDLTWLMTDSVIKNFFWLQTDAPGKQQEIDAKCYSNNLAVTVTTNVTSATVL
                     LDH"
BASE COUNT          542 a          634 c          628 g          468 t
ORIGIN      
        1 ccacccattt gttcgcctta ccgtgattgg gttggggatt ctgcaactca cgttggtggc
       61 aacaattgcc cgcgccgaaa cgaacacaaa gtctccatgg cctgacgctg ttgaacaagc
      121 gctcgctcaa agcggcacca atcgggcaag ccttgaagcc gccttccaac aagcgcccgc
      181 taatgtgcgc agagatttcg aattcattct ggaaaacatg cccacacagg atttgcaatc
      241 cttatcgggc acatttctac tcgatgatgc gcgactcgcc cgcgaaagct ttcttgccgc
      301 gccctggcac gagcgcattc cgcaggatat ttatctgaac gacattctcc cttacgcttg
      361 cgccaacgaa acccgcgaag ggtggcgaaa ggttctgcgc gaaaaatgcc tgccgttgat
      421 cgctgattgc aagacgcccg ccgaagccgc gcaacggctt aacgaaaacc ttttcaaact
      481 cacaaacgtc aaatattcca ccgaacggaa aaaggccgac caaagccctt ctgagacgat
      541 ggagagcgga ttggccagtt gcacgggcct gtccattttg ttgattgatg cctgccgttc
      601 cgtcggcgtg cccgcgcgat tggcggggac gccgatgtgg gtcaacatgc gcggcaatca
      661 tacctgggtc gaggtgtggg acaccgactg gcatttcacc ggcgcagccg agccggacgc
      721 gaagggattg gaccacacct ggtttgagca cgacgcttcg gaagcgcaaa aagacaatcc
      781 gcaacacgcc atctatgcgg ccagtttcaa aaaaacggga ttgtcctttc cgctcgattg
      841 ggcgccggac atccattggg tcagcgccgt caatgtgacc gaccgctata cgcccaaagc
      901 cgccgctcca gagcaggaca aagtgcgttt gatggtcaag gtgctcgacc atcttgccgg
      961 tcaacgcgtg gaagcgcggg tcatcgtctc gaacatcgct gatgggaaaa tccattttga
     1021 aggagtcagc aagggtgaat cagtcgacat gaatgagttg cttgcgttcg acgttccgcg
     1081 tggagcaacg tatgatattc gcgcggagca gttcggccag gaagccgggc gcgaattcat
     1141 cgcggggaca aattcgcagg cagtcgtagt gatttcgctg aacgacacca cgttgttcaa
     1201 atttccatct caagcctgtt acgtgccgcc cgcagtgagc cattcgctca aaccgcgcga
     1261 cgaagaaaaa ttgaaagcgg ctctcgtgga attttttgcg gctccttcaa aaaagcaagc
     1321 cacatggaaa tttcccagga gcatggaaaa gctgttgcgc gaaaacgagc cagccgtgcg
     1381 ccgcgttgca tgggaggctt acgtcgccgc gcctatccat gaaacgatga aaaaagattt
     1441 tgacgggcat caagcacggt tcgaaaccaa tttgagcccg ttcacggtga ggactgtcgg
     1501 tgaacggccc gcaaaaggtt gggcgttgtt catcgcgatg catggcggcg gaggcgtgga
     1561 aaaagaggtg aacgacagcc aatggaaaat catgcagcat tattacaagg accatccgga
     1621 ggctggcggc tatctttacg tggcgttgcg cgcgccgaac gacacttgga acgggtttta
     1681 tgatgattac gtttatccgc tggttgccaa tctggtgcgg gagtttttgc tgtacggcga
     1741 tgtggacccc aacaaggtgt tcatcatggg atattcacac ggcggttatg gcgcattcgc
     1801 aatcgggccg aaggaacccg atcttttcgc cgccattcac gcaagcgccg ccgcgccgac
     1861 ggatggcgaa accaccggcg tcacgctacg caacaccatc ttcacctgca tggtcggcga
     1921 gaacgacacc atgtatggcc gcatcgagcg tgaccgaaaa ttcaaggatg ccatggcgca
     1981 attgcgcgcc aaagatggca ctttttatcc cgtgacggtg cagatcatcc ccggcaacgg
     2041 ccatactggc ttgcccgacc gcgacaagat caaggagatg tatcctgcgg tgcgaaaccc
     2101 cgtcccgcac gatctcacct ggcttatgac cgacagtgtc atcaaaaatt tcttttggct
     2161 gcagaccgat gcgccgggca agcagcagga aattgacgcg aagtgctatt ccaacaatct
     2221 cgctgtgact gttacaacca atgtcacttc cgcgactgtt ctccttgatc at
//
LOCUS       DASZMM010000458         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_764606, whole genome shotgun sequence.
ACCESSION   DASZMM010000458 DASZMM010000000
VERSION     DASZMM010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764606"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VG754_11010"
     CDS             <1..685
                     /locus_tag="VG754_11010"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HWF19791.1"
                     /translation="GANRAAAFDIEAACSGFIYALEIGQQFIMSRTYNTVLVIGAEKL
                     SSIVNWTDRNTCVLFGDGAGAAILQNDPNSHGLLTACMGADGQKADLLSMPAGGSRCP
                     ASVESVQAGLHFLRMDGKETFKNAVQAMLTAAQESLRRCEIDITQIKCIIPHQANQRI
                     IDAVSERLGAKPEQVFVNLHKYGNTSAASVAIALHEAVESGRIQRGDLILLIVFGAGL
                     TWGAAVIEW"
     gene            983..1147
                     /locus_tag="VG754_11015"
     CDS             983..1147
                     /locus_tag="VG754_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19792.1"
                     /translation="MTVTLQSTKDAGRVHCTGVVVACNGNRHNGYIVSMVFTSLSKQS
                     QARLNLLAFP"
     gene            1190..2278
                     /locus_tag="VG754_11020"
     CDS             1190..2278
                     /locus_tag="VG754_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWF19793.1"
                     /translation="MELFNRILKLAVDGGASDIHLKIGTPVIFRISRQLIAIECPLPA
                     EEWMNNVVKNITPPHLQKRLEMEREVDFSYYSPGIGRFRTNLFQQRGQFCLAMRFVKT
                     NVPSFQELGLLETIKNIAESPRGIVLVAGSTGSGKSTTLAAMIEHINAHFKKHIVTME
                     DPIEFVFEDNQSVIEQREVGLDTLSFEHALKHVLRQDPDIIMIGEMRDATSFTAAMSA
                     ADTGHLVLSTLHTTNAAQSISRILDFFKAEEREQVRRQLAGTLQAVVCQRMVGTVTGG
                     MTPALEILINTGTVKKLMEENRLDKLPAAIETAADDGMLNFNQALFQLVKEGKVSQKE
                     ALAKASNPQALEMNFKGIFLDEGRRILG"
     gene            complement(2288..>3365)
                     /locus_tag="VG754_11025"
     CDS             complement(2288..>3365)
                     /locus_tag="VG754_11025"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HWF19794.1"
                     /translation="VSTTTTTAMGELQKRLPPSIGKIYYPFDHSSSVARALNTIHPNA
                     IILVESEIWPNFIWRARALGKPLFLVNARLSDRSYPRFKKFGFLFKNLFGAFTSVGAQ
                     NEDDAAKLRELGCAAEAVRVVGNLKFDAAKPVGNHPLDVAGLLKQLGVSADAPLWVAG
                     STHPGEERILAGQFQRLRTQFPDLVFILVPRHNERAREVGQELSELGIKYVYRSEIAP
                     DTQRHAGSVQCLLVDTIGELRYFYEHATVVFVGKSLVAEGGQNPIEPAALGKPVVFGP
                     NMQNFTDVTKNFLAQGGAIQVRDADELEKTIADLLGNPKRRAQLGCNAQRIVQENQGA
                     VERTVEMILENLKDREFYIVPSSK"
BASE COUNT          776 a          902 c          913 g          774 t
ORIGIN      
        1 tggggctaat cgcgccgccg catttgatat agaggccgcc tgctctggtt ttatttacgc
       61 actcgaaatc ggccagcagt tcatcatgtc ccggacttac aacacagtcc tcgtcattgg
      121 cgcggagaaa ctttcttcca tcgtcaattg gactgaccgc aacacctgcg tgctttttgg
      181 cgacggcgcg ggcgcggcga ttttgcaaaa cgacccaaac tcgcacggct tgcttaccgc
      241 gtgcatgggc gcggacgggc agaaggctga tttgctttca atgcccgctg gcggcagccg
      301 ttgtcccgcg agcgtggaat ccgtgcaggc tggattgcat tttttgcgga tggacggcaa
      361 ggaaaccttc aaaaatgcgg ttcaggcaat gctcaccgcc gcgcaggaat ctttgcgccg
      421 ttgcgaaatc gacatcacac aaataaagtg catcattccg caccaggcaa atcagcgcat
      481 cattgatgcc gtcagtgaac ggctcggggc gaaaccagag caggtttttg taaacctgca
      541 taagtacggc aacacttcgg cggcgtcagt tgcgattgca ttgcacgaag cggtcgaatc
      601 tggccgcatc caacggggag atttgatttt gctgatcgtt tttggcgcgg gacttacttg
      661 gggtgcggca gtgattgagt ggtgatttac cgacttacga tttgcccaag attgcagtgc
      721 catcagggca aatttactta ctcaaggtat aaatttccta caagttgacc ctaaaaccca
      781 tttgacgaag acctgctttg tgataaggtg gattactgct ttttaattta tgagtgcaag
      841 aaacattgga tcgtccggtg catttgaacc agtaacagtg cagacgcggc aaacgcgttt
      901 gtccctttcg cctgagcatg tccggatcca caggaacggc gttgagtttt gctcaagctc
      961 ccccatcagt gcctggacag aaatgactgt cacgctgcaa tccacgaaag atgctggccg
     1021 cgttcattgc acgggagtgg tggtggcctg caacgggaac cgtcataatg gttatattgt
     1081 ttcgatggtt ttcacgagct tgtccaagca atcgcaggcg cgactgaatc tgctggcttt
     1141 cccctgaccc gtcttgcctt gtgtgggacg tgtgtgttaa accatcgcca tggaattgtt
     1201 caatcgtatc ttgaagctcg ctgtggatgg cggcgcttcc gacattcacc tcaaaatcgg
     1261 cacaccggtc attttccgca tcagccgcca gctcatcgcc attgaatgcc cccttcccgc
     1321 cgaggaatgg atgaacaacg tggtgaaaaa catcacgccg ccgcatttgc aaaaacggct
     1381 ggaaatggaa cgcgaggttg atttctcgta ttattcgccg ggcatcggac ggtttcgcac
     1441 caatcttttc cagcaacggg ggcagttctg cttggcgatg cgttttgtga aaacaaacgt
     1501 gccgagtttt caagaactcg gtttgctcga aacgatcaaa aacattgccg aatctcctcg
     1561 tggcattgtg ctcgtggccg gctcgaccgg cagcggcaaa tccaccacgc tggcggcgat
     1621 gatcgaacac atcaacgcgc atttcaaaaa gcacatcgtg acgatggagg acccgattga
     1681 attcgtgttc gaagacaatc aatcggttat tgaacagcgc gaagtcggcc tggacacgct
     1741 gtctttcgag cacgcgctca aacatgtgct gcgccaggac ccggatatca tcatgatcgg
     1801 cgaaatgcgg gacgcaacaa gtttcacggc cgccatgagc gcggccgaca ctggtcactt
     1861 ggtgttgtcc acccttcaca ccacgaatgc cgcgcaatcc atcagccgca ttctcgactt
     1921 tttcaaggcg gaggaacgcg agcaagtccg gcgtcaactt gccggcactt tgcaggcggt
     1981 cgtctgccag cgcatggtgg gaaccgttac cggcggcatg acacccgcgt tggaaatttt
     2041 gatcaacacc gggaccgtga aaaaattgat ggaggaaaac cgcctcgata aactgcccgc
     2101 tgccatcgaa actgcggctg atgatggaat gctgaatttc aaccaggcgc tgttccagtt
     2161 ggtgaaggaa ggcaaggttt cccagaagga ggccttggcc aaagcgagca acccgcaggc
     2221 gctcgaaatg aatttcaaag gtatcttcct cgacgaaggc cgccgtatcc tgggctgaga
     2281 ttacaatcta tttcgacgac gggactatgt aaaactcgcg gtccttgagg ttttccaaaa
     2341 tcatctccac cgtgcgctcg actgcgccct gattttcctg gacaatccgt tgcgcattgc
     2401 agccgagttg agcgcggcgt ttcggattgc caagcaaatc ggcgatggtc ttttccaatt
     2461 cgtccgcatc gcgcacctgg attgcgccgc cctgcgcaag aaaatttttt gtgacgtcgg
     2521 tgaaattctg catgttcgga ccaaatacga ccggctttcc caatgccgcc ggttcaattg
     2581 gattttgtcc accttcagcc acgagactct tgcccacgaa gacgaccgtt gcgtgttcgt
     2641 aaaaatatcg caactcgccg atggtatcca caagcaggca ctgcaccgag ccggcatgtc
     2701 gttgcgtgtc cggcgcaatc tcgctgcgat aaacgtattt gatcccaagc tcggaaagtt
     2761 cctgtccgac ttcgcgcgca cgttcgttat gacgtggcac taggatgaaa accaagtcgg
     2821 gaaactgcgt gcgaagacgt tgaaactgac cggccaaaat ccgctcctcg ccgggatgcg
     2881 tgctgccggc cacccataaa ggagcgtctg ccgatacgcc caactgcttc agcaatccgg
     2941 caacatcaag cggatgattg ccgaccggtt ttgcggcgtc gaactttaga ttgccaacca
     3001 cgcggacggc ttcagccgca catccaagct cgcgcagctt ggccgcatca tcttcgtttt
     3061 gagcacccac tgaggtgaaa gcgccaaaga ggttcttgaa caggaaaccg aattttttga
     3121 aacgcggata agaacgatcc gaaagacggg cattgaccag gaacaatggt ttgccgagcg
     3181 cgcgagcgcg ccagatgaaa ttgggccaaa tctccgattc aacgagaatg atggcgttgg
     3241 gatggattgt gttgagagcg cgagcaacgg aactgctgtg gtcgaatgga tagtatatct
     3301 ttccaatgct tggaggcagc cgtttttgca attcgcccat cgctgtcgtg gtggtggtgg
     3361 atacc
//
LOCUS       DASZMM010000459         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2402891, whole genome shotgun sequence.
ACCESSION   DASZMM010000459 DASZMM010000000
VERSION     DASZMM010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402891"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..297)
                     /locus_tag="VG754_11030"
     CDS             complement(67..297)
                     /locus_tag="VG754_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19795.1"
                     /translation="MSYLTLEVEIDHGKVITKKPAKLPEKASGSLTILEPANQPGGSL
                     TPLQAFEELQKHLQLDPQKAQEWMDAVREGRR"
     gene            complement(419..>1771)
                     /locus_tag="VG754_11035"
     CDS             complement(419..>1771)
                     /locus_tag="VG754_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19796.1"
                     /translation="FRKLGAQDIGGYKRAGGTEPMPRSLLLIDEFQELFVEDDKISQG
                     ASVLLDRIVRQGRAFGIHVILGSQTLGGAYTVARTTLGQMVIRIALMCNEADAYLIMD
                     DSNPAPRLLTRPGEGIYNDMAGALEGNSPFQTVWLSDDVRDQYLDKVRQRAIQSKIAY
                     AGPIVFEGDAPAEVRENQLLQSLLEVDAIQKPVSPRIWLGAPNSIKGPTEAVFHRQSG
                     NNMLIVGQRDEAALAMLSIGLVALAAQYPRSTAKFILFDGTPPGSPHREFLEKIVSII
                     PHPIVVAKHADLAEIMNGLAEDMKHRMEADHGAGAPETFLLFHGLQRQNKLRYEEDFS
                     FSADDAAANPAAQLNKLITEGTSLGFHVIATCDTYNNVNRFLSKKAFSEFELRVLFQM
                     SVNDSANLIDSPKAGNLGLHRALFYNEQEGWLETFRPYALPDNEWLEQAAQNLMRLIK
                     "
BASE COUNT          376 a          489 c          490 g          416 t
ORIGIN      
        1 atcaaatcct ggtggcttgg acgtagattt gaattatgaa gttggtatcg aaatgaatca
       61 tgaatgtcat cgtcttcctt cacgcacggc atccatccac tcctgagcct tttgagggtc
      121 taattgaaga tgcttttgaa gttcttcgaa tgcctgcagc ggggtcaagc tcccgcctgg
      181 ctgattcgcg ggttctagaa tggtgagcga gccgctggct ttctccggca atttcgcagg
      241 ctttttcgtg atcacttttc catgatcaat ttccacttcc agtgtgagat aactcatggc
      301 aaaaaaataa ccgctctgcc gcgaatggcg agccaaattt cggcaacgcg cgatcctaaa
      361 aaatctttca aacccgtccg cacaaatgaa tgcgatgccg acttggggca atactttctt
      421 attttatcag ccgcatcaaa ttctgcgcgg cctgttcgag ccattcgttg tcgggcaatg
      481 cataagggcg gaatgtttcg agccagcctt cctgttcgtt gtaaaagagc gcgcggtgca
      541 ggcccagatt tcccgccttg ggactgtcaa tcaggttggc cgaatcgttc acactcatct
      601 ggaagaggac acggagttcg aattcgctga acgccttctt gctgagaaaa cgattcacgt
      661 tgttgtaggt gtcgcacgtc gcgatgacgt ggaacccaag gctcgtccct tcggtgatca
      721 acttgttcaa ctgcgcggcg ggattggcag ccgcgtcgtc agccgaaaag ctgaagtcct
      781 cctcgtaacg aagcttgttc tgccgctgca aaccgtggaa aagcaaaaaa gtttccggcg
      841 cgcccgcgcc gtggtcggct tccatccggt gcttcatgtc ctcggcaaga ccgttcatga
      901 tttcagcaag gtcggcgtgc ttggcaacca cgatgggatg cggaatgatg ctgacaatct
      961 tttccaaaaa ttcgcggtgc ggcgaaccgg gcggcgtgcc gtcgaacaaa atgaatttcg
     1021 ccgtgctgcg cggatattgc gccgcaagcg cgacaaggcc gatgctcaac attgccagcg
     1081 cagcttcgtc gcgttgaccg acgataagca tgttgttccc gctttggcga tggaacaccg
     1141 cctcggtcgg tcccttgatg gaattgggcg cgccgagcca tattcgcggc gacacgggct
     1201 tttgaatcgc gtccacttcg agcaaacttt gtaacaattg gttctcgcgc acttcagcgg
     1261 gcgcatcgcc ttcgaacaca atcggcccgg cataagcgat tttgctttgg atggcgcgct
     1321 gccgcacctt gtcgagatat tggtcgcgca cgtcatcgga aagccagacc gtttggaatg
     1381 gactgttgcc ttccaatgcg ccggccatgt cattgtaaat gccttcgccg ggacgcgtga
     1441 gcaatcgcgg cgcgggattg ctgtcatcca tgatgagata agcatcggcc tcattgcaca
     1501 tgagcgcgat gcgaatgacc atttggccaa gggtggtgcg cgccacggtg taagcgccgc
     1561 caagcgtttg cgagccaaga atcacgtgga tgccgaacgc gcggccttgc cgaacgatgc
     1621 ggtcgagcaa caccgatgcg ccctgcgaaa tcttgtcgtc ctcgacaaac aattcctgga
     1681 actcgtcaat cagcagcaac gaacgtggca tcggttccgt gccgcccgcg cgtttgtaac
     1741 cgccgatgtc ctgcgcgccc agtttgcgga a
//
LOCUS       DASZMM010000460         7576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4045483, whole genome shotgun sequence.
ACCESSION   DASZMM010000460 DASZMM010000000
VERSION     DASZMM010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4045483"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VG754_11040"
     CDS             complement(<1..1296)
                     /locus_tag="VG754_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HWF19797.1"
                     /translation="MADASATFSESWYRIAGKHVYLRAGVRVQRQSYRGERWIVLQNP
                     FSNQYFRVRPAAYEFVARLSPDKSVQEAWQECVNRFPDEAPGQEAVIQLLSQLYYANL
                     LHYPDAEDSAQLFERVKRRRQREMSARLMNLMFLRFPLLDPDRFLVATMPVVGKFISK
                     FGALLWFVVVGFAVKVVVDNFTAVKLQGQGILSPGNLPLLYVALVGIKSIHEFGHAYF
                     CRKFGGEVHVMGILLMIFTPVPYVDASSSWGLRNRWHRVLVGAAGMIVELFVAALATF
                     VWVKTGDGALHSLAYNVMFIASISTLIFNANPLLRFDGYYILSDLLEIPNLSQRASAQ
                     LRHLWEHYIFGVKDSEGAATKTGEAAWLVVFGITSGIYRVIVFAGVLLLVADRFLLLG
                     LIMAAVCAVSWVTVPVGKFIHYLASSPRLERVRWRATAAT"
     gene            complement(1300..3162)
                     /locus_tag="VG754_11045"
     CDS             complement(1300..3162)
                     /locus_tag="VG754_11045"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF19798.1"
                     /translation="MAQESQTTPANLEQALVELARLREFAGPPKEFWPRFLAALHQLT
                     LADNWVVLARKPGAPWRRMLNWPAEALPSAMLTAFSTRVEEFANRPGATGGQVISLEE
                     KPGRSSGNFVLACGLELPPPAEECVLVGLVSEVSEAQAREALVRLRLAAATPGHYQAG
                     LGARQAKADVEKFACVLDLTVSFNSEKHFVAAALSFCNGLATRFNCDRVSLGWLDGGY
                     VRLRTMSRTEKFDRQMAAARELETAMEEAFDQDEEVIWPAPEGSTVITRDHERFAKEQ
                     KAGNICSLPLRADNEPVAVVSCERQNAPFTPVELQQIRLACDLAAPRLSELQQQDKWF
                     GARYAAQFRKQCARFIGPEHTWAKVVMLLSAVVLALLFFLRVPYRVQGNFVLRSDELA
                     YVTAPYDGYIDKVLARPGDALTAGAPLLKLKTSELELEKSFAEADLNRYVREAEKARA
                     ARSLGEMRVAEAVADQARSRLDLSKYRLEEAVIRTPFPGVVIEGDLRERLGSPVKMAD
                     VLFKVARLDTLYVEAEVNERDVHEILGKTSGEIAFLSRPRDKFPVRILTVEPAAVPKN
                     EANVFLVRCAIEGGAQPWWRPGMSGVCKFKVEKRSLFWILTHRTVDFLRLKLWW"
     gene            complement(3172..3915)
                     /locus_tag="VG754_11050"
     CDS             complement(3172..3915)
                     /locus_tag="VG754_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF19799.1"
                     /translation="MKLAASKMAVTLGLCLALGVSQMARAGAQNCEGITEPVFDVTLS
                     MAVPGIMTVQRFKEGQFVQTNDVILELDSQLEELEVERRRLVMENRKADADSTKMVFD
                     KTASVSRDELLKKEADYEVALAEYKIAVQELQRRKLIAPGAGVITEISLHVGEACSPY
                     TPVARLVDTRQCYFISNIEAKAAAGLKTGEMVQLEIDDLKNPIKLGAKIVFVSPVVDS
                     ASGLQKIKAIFDNADGKVRPGVTGRMLFD"
     gene            complement(4398..6161)
                     /locus_tag="VG754_11055"
     CDS             complement(4398..6161)
                     /locus_tag="VG754_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF19800.1"
                     /translation="MRGKETIHHPPFKKLRSAQLALGIGLLLCATLRLDAEISPPPGG
                     ASGLTLSNLVQLVLERNETLQSKLLDFEVSRQRHLAEKGVFEPDLVGSFNHEVNNRPN
                     NAEQQNTDQNSVLHETNNIYDGGLESLIPTGAKLHLGYTLSDLHNNIFPSGLFSGGLT
                     SNLNQLQYQTFIGVTVDQPLLKNFGPAVTMAGIRIAALSSKIAFQEYRRQLMTVVSTA
                     EATYWNLYLSQQQEQFFEDSVHTAETILNDNRTRLEAGKGSELEVMEAQAGLGLRRAK
                     LGEAQQKVVEAANRVVSFYAGRADGTNQFLRALDAPTLREEHMDFDFLGSIAFTANPD
                     YVIQEEKVDQELIKLGYARNQRLPQFDLKGAYGYNGLGATPEESWDAAERTHFLSWSI
                     GAEFRIPLGATKVRHEYTAAELQVQSAELALQGLQTEIFNGLDTARHKVLSLRDSVAS
                     YQSAVDYNRTLLDSAMTRLQAGKLESRKVLDIEADLFEARNSVVESLVRYQIAWLEMQ
                     VLSGGLLRQRNLDISQEDLQRTTRDMLKDRGSSGANFLHGNNEQNAYYPKGVGASPYN
                     DSALRDALHRKESELENPSGK"
     gene            complement(6677..>7576)
                     /locus_tag="VG754_11060"
     CDS             complement(6677..>7576)
                     /locus_tag="VG754_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HWF19801.1"
                     /translation="STFYGMTVQGGSNGYGVIYSISTNGSNFNVLHHFAAATGGTPVG
                     NLVLSGNTLYGTTEVGGSNNLGTVFAIGTDGNNYSQLYAFGPVPDGNMPQCTLALSGN
                     TLCGTTSAGGSNSVGTLFTINTDGTDYQIVQNFAVDGANPVGSLALSGTNVFGITTGG
                     QAGAGTVFELGLPPAPTYPAPFFAGEVPLPPYTNGWYWMGPQGANTYGFGYFNTANFP
                     YVLHNDMGWEYFIDANDAGSGGYFYDFTDEAWFYTTPGLFPYLYDFNQNAWVYYLPES
                     SYIYSSSPRWFDNLSTLIWSNDL"
BASE COUNT         1679 a         2272 c         2023 g         1602 t
ORIGIN      
        1 tgttgcggct gtcgcacgcc aacgcacccg ctccagacgc ggactcgacg ccagataatg
       61 gatgaacttg cccaccggca cagttaccca tgaaacggcg caaaccgccg ccatgatgag
      121 tccaaggagc aaaaaccgat cggccaccaa caacaacact cccgcaaaaa caatcacccg
      181 ataaatccca ctcgtgatcc cgaaaaccac cagccatgct gcttcgcccg tcttggttgc
      241 cgctccttcg gaatccttca cgccaaaaat gtaatgctcc caaagatgcc gcaactgcgc
      301 gctggcgcgt tgcgataggt tcgggatctc cagcaggtcg gacaaaatgt aatagccgtc
      361 aaagcgcagc agcgggttgg cgttgaaaat gagcgtcgag atcgaggcta tgaacatcac
      421 gttgtaagcc aggctgtgca gcgcgccgtc tcctgtcttg acccagacaa acgtggccaa
      481 tgccgccacg aacaattcca caatcattcc tgccgcgccc accagcacgc gatgccagcg
      541 attgcgcaat ccccagctcg acgacgcgtc cacatacggg accggcgtga aaatcatcag
      601 caaaatgccc atcacatgca cttcgccgcc gaacttgcgg caaaaataag cgtggccgaa
      661 ttcgtggatg cttttgattc ccaccaacgc gacataaagc agcggcagat tgcccggcga
      721 gagtatcccc tgcccttgca atttcaccgc ggtgaaattg tccaccacga ccttgaccgc
      781 gaacccgacc accacgaacc acaacaacgc gccaaatttg ctgatgaatt tgccgaccac
      841 gggcatcgtg gcgacgagaa atcggtcggg gtcgagcaat ggaaagcgca ggaacatcaa
      901 attcatcagc cgcgcgctca tttcgcgctg gcgacggcgc ttgacccgct cgaacaattg
      961 cgcgctgtct tccgcgtcgg gataatgcag caggttcgcg taataaagtt gggaaagaag
     1021 ctggatcacc gcctcctggc cgggcgcttc atcgggaaac cggttcacgc attcctgcca
     1081 ggcttcctgg acgcttttgt cgggagacaa gcgcgccaca aactcatacg cggcgggacg
     1141 cacccggaaa tattggttgc tgaacggatt ttgcagcacg atccagcgtt cgccccggta
     1201 actctgccgc tgcacccgca cgcccgcgcg gagatagaca tgctttccgg cgatgcgata
     1261 ccaggattcg ctgaacgtgg cggatgcgtc agccatggtt caccaccaaa gtttcaaccg
     1321 caggaaatcc accgtgcgat gcgtcaatat ccagaacaac gaccgcttct cgactttgaa
     1381 cttgcagacg ccgctcatgc cgggacgcca ccatggctgc gcaccgccct cgatcgcgca
     1441 acgaacgaga aaaacatttg cctcgttctt cggcactgct gccggttcaa cagtgagaat
     1501 ccgcaccggg aacttgtcgc gcggacggct caggaaagcg atttcgccgg aagtcttgcc
     1561 gaggatttca tgcacgtcgc gctcattgac ttccgcctcc acataaagcg tgtccaaacg
     1621 ggcgacttta aacaacacgt ccgccatctt cacgggcgag ccaagccgtt cgcgcaaatc
     1681 gccctcgatg accactccgg gaaacggagt ccggatgacc gcctcttcca gccggtattt
     1741 tgaaaggtcc agccgcgacc tggcctggtc ggcgacggcc tcggcgacgc gcatttcgcc
     1801 cagcgatctc gcggcgcgcg ctttctcggc ttcgcgcacg taacgattca agtccgcttc
     1861 ggcaaaagat ttttccagtt caagctcgct cgtcttgagt ttgagcaacg gcgcgcccgc
     1921 ggtcaaagca tcgcccggtc gcgccaaaac cttgtcaatg tatccgtcat aaggcgcggt
     1981 cacataggcc agttcatcgc tgcgcaaaac gaaattgccc tgcacccgat aaggaacgcg
     2041 aaggaaaaag agcaacgcaa gcaccacggc gctcaacaac atgaccactt tggcccatgt
     2101 gtgttccggc ccgataaagc gcgcgcattg tttgcgaaac tgggcggcat aacgcgcccc
     2161 gaaccacttg tcttgctgtt gcaattccga aagccgcggc gcggccaaat cacaggccaa
     2221 ccgtatttgc tgaagttcca ccggcgtgaa gggcgcgttt tgccgttcac acgaaaccac
     2281 cgcaacgggt tcattgtccg cccgcaatgg aagcgagcag atgtttccgg ccttctgctc
     2341 tttggcaaac cgctcgtgat cgcgggtgat gaccgtggaa ccttccggcg cgggccaaat
     2401 cacttcctcg tcctggtcga acgcctcctc catcgccgtt tcaagctcac gcgcggcggc
     2461 catctggcgg tcgaattttt ccgtgcggct catggtgcgc aaccgcacat aaccgccgtc
     2521 cagccagcca aggctcaccc ggtcgcaatt gaatcgtgtc gcaaggccgt tgcaaaatga
     2581 caatgccgca gcaacaaaat gtttttcgga attgaagctg acggtcaggt caagcacgca
     2641 cgcaaatttc tcgacatccg cctttgcctg ccgcgcgccc aagccggcct ggtaatgtcc
     2701 cggcgttgcc gctgccaatc gcaatcgcac cagcgcttcg cgggcctgcg cttcgctgac
     2761 ttctgaaacc agccccacca gcacgcattc ctcggcgggc ggcggcagtt ccaatccgca
     2821 ggcgagcaca aaatttcccg acgagcggcc cggcttttcc tccaaggaaa tgacctgtcc
     2881 gccggttgcg ccgggacggt tcgcaaattc ttcgacccgc gtggaaaagg cggtcagcat
     2941 cgcggaaggc agcgcttccg ccggccagtt gagcatccga cgccacggcg cgcccggttt
     3001 gcgcgccagc accacccaat tgtccgcaag ggtcaattga tgcaacgccg ccaggaaacg
     3061 cggccagaat tccttcggcg gcccggcgaa ttcgcgcaaa cgcgccagtt cgacgagcgc
     3121 ctgctccaga ttcgccggtg tggtttgaga ttcctgggcc atgaatgatc ctcaatcaaa
     3181 caacatcctg cccgtcacgc cggggcggac ttttccatcc gcattgtcga aaatcgcctt
     3241 tatcttttgc agcccgcttg ccgaatccac gaccggcgag acaaacacga ttttcgcccc
     3301 gagtttgatg gggtttttca aatcatcaat ttcaagctgg accatctcgc cggtcttcag
     3361 cccggcagcg gcctttgcct cgatgttgct gatgaaataa cattggcgcg tgtccaccaa
     3421 acgggccacc ggcgtgtagg gagaacaggc ttcaccgaca tgcaggctga tttcggtgat
     3481 gactcccgcg ccgggcgcaa tgagtttgcg gcgttgaagt tcctggactg cgatcttgta
     3541 ttccgcgagc gccacttcgt aatcggcttc ctttttcagg agttcatcgc ggctgacgga
     3601 tgcggtttta tcaaagacca tcttggtgct gtccgcgtcg gccttgcggt tttccatcac
     3661 gagccgccgc cgttccactt ccagttcctc caattgcgaa tccagttcca ggatgacgtc
     3721 gttcgtctgc acgaattgtc cttccttgaa ccgttgcacg gtcatgatgc cgggcacggc
     3781 catgctgagg gtgacatcga acaccggttc ggtgatgcct tcgcaattct gcgcgccagc
     3841 ccgcgccatt tgcgaaacgc caagcgcaag acataaaccc aatgtcacag ccattttgga
     3901 tgctgccagt ttcataccat ttttatcatg ccggaacgcg aacggattaa aagcttaaag
     3961 gacaattttc aaaaaaattt ctgacgtttt gcattgacgc gccacaaatt gggcgcggaa
     4021 acggagcgcg gctcgatgag ccgcagcaac gcatggcaat caagacacgc ccgactgatt
     4081 catctgcgcc cgagcttccc acattgctgc gggtcaccga cccgcgctcc aaaagctgcg
     4141 gctggcgctt tgcgccacgt cgcgctccat tatttatgta agcgcccatc gtatcacgga
     4201 tgcgtcgggc catcgtaccg gagtggggac tggaaagtcc ccacaacccg caggctggaa
     4261 agcctgcgcc acgtagcgca gacattcttg tctgcgggtt caggcgactt tccagtcgcc
     4321 tgaattcttg cacctgcaat ccagaatcaa gaacagtttc aagatgcgcc ttcgtttaga
     4381 gaattttcat ccatccgcta ttttccggac ggattctcaa gttccgattc ttttcgatgc
     4441 aaggcatccc gcaacgctga atcgttgtaa ggcgacgcac cgacgccttt cgggtaataa
     4501 gcgttttgct cattgttgcc gtgcaaaaag ttcgcgccgc tggacccgcg gtccttgagc
     4561 atgtcgcgtg tggtgcgctg caaatcttcc tgcgagatat ccagattgcg ctggcgcaac
     4621 aatccgccgg agagcacctg catctcaagc caggcgatct gataccgaac cagcgattca
     4681 acgaccgaat tgcgcgcttc gaacaaatca gcctcgatgt ccagcacctt gcggctttcc
     4741 agtttgccgg cctgcaatcg cgtcatcgcg gaatcgagca gggtgcgatt gtaatccacc
     4801 gccgattgat agctcgccac gctgtcgcgc aggctcaaca ccttgtggcg cgcggtgtcg
     4861 agtccgttga agatttcagt ttgcagtcct tgcaacgcca gttcggcgga ctgcacctgc
     4921 aattccgcgg ccgtgtattc atgccgcacc ttcgtcgcgc ccaatgggat gcggaattcc
     4981 gccccgatgg accatgatag aaaatgggtg cgttcggcag cgtcccacga ttcctcgggc
     5041 gtcgcgccaa ggccgttata accataagcg cctttcaaat cgaattgcgg aagacgctgg
     5101 ttgcgcgcgt aaccaagctt gatgagttcc tggtccactt tttcttcctg gatgacgtaa
     5161 tcgggatttg ccgtgaatgc gatgctgcct aaaaaatcga aatccatgtg ctcctcgcgc
     5221 agcgtcggcg catccaacgc ccggagaaat tgattggtcc cgtcggcgcg cccggcataa
     5281 aacgagacga cgcggttggc ggcctcgacg actttttgct gcgcctcgcc aagtttcgcg
     5341 cgccgcaagc caagtcccgc ctgcgcttcc atgacttcga gttcggaacc tttgcccgct
     5401 tccagccggg tgcggttgtc attcaggatg gtctcggcgg tgtggaccga atcctcgaaa
     5461 aattgttcct gctgctgcga gagataaagg ttccaataag tggcttcagc ggtggacacc
     5521 actgtcatca actgacggcg atattcctga aaagcgatct ttgaggaaag cgccgcgatg
     5581 cgaatgcccg ccatcgtgac cgccggcccg aaattcttga gcaacggctg gtcaacggtg
     5641 acgccaatga acgtctggta ttggagctgg ttgagattgg aagtgagacc gccgctgaaa
     5701 agtccgctgg gaaaaatatt gttgtgcaaa tcgctcaagg tgtaacccag atgcaacttt
     5761 gccccggtcg ggatcaacga ttccaagccg ccatcgtaaa tattgttcgt ttcgtgcagg
     5821 acgctgtttt ggtccgtgtt ttgctgctcg gcgttgttgg ggcggttgtt gacctcatga
     5881 ttgaaagagc cgaccagatc cggctcgaac acgccctttt ccgcaagatg cctctgccgg
     5941 ctgacttcga aatccagcag tttggattgc agggtttcgt tccgttcgag gacaagctgg
     6001 accaggttcg agagggtcaa gcctgacgcg ccgccgggcg gaggggaaat ctcggcatcc
     6061 aggcgcagcg ttgcgcatag caaaagcccg atgcccaatg ccagttgcgc gcttctcagt
     6121 tttttgaacg gtgggtggtg gatcgtttcc ttccccctca ccccgaccct ctcccttggg
     6181 gagagggagc aatttcgccc gctgtggttg caatcctcgg cgttggattg tgcaaacgcg
     6241 gccaaacatt caccctctcc tcggggagag ggaaagggtg agggggaccg cgacgcgttc
     6301 catcccgcaa acacaaaccg aaaagggcct gcagcatgca gaaaaatgag aagcgcccgt
     6361 gccagtttga aacttttgat gattacagcc actaattttt ttagcgcggg caatccgtga
     6421 tttcaatgcc actttcaacc catggcaaga cacttcacgc ccgcattttt ttatctttct
     6481 tttacgatgg cgcgcccgac ggtttgcacc gaaaggcatc gcctgcacgc aaccaaaaga
     6541 tgcacccaaa tggttgattc agactttgtt ggatattata tgaaccggat aaaccgtgtc
     6601 catcaaaaac agtgcggagc agggagaaaa agtgcagatc cagatgcaat ggcggattgt
     6661 cgggtcagcg tcgcgcttac aggtcgttcg accaaattaa ggttgataga ttatcaaacc
     6721 aacgcggact ggagctgtag atatacgatg attccggcaa ataatagacc caggcgttct
     6781 ggttgaaatc gtaaaggtaa ggaaacagac cgggcgtggt ataaaaccat gcttcgtccg
     6841 tgaaatcgta gaaataaccg ccgctccccg cgtcgttggc atctatgaaa tattcccagc
     6901 ccatgtcgtt gtgcaaaacg taggggaagt tcgctgtatt gaaatagcca aagccatacg
     6961 tgttggcgcc ttgaggtccc atccaatacc aaccgttggt gtagggcggt aatggaactt
     7021 cgccggcgaa aaacggcgct ggatacgtcg gggctggcgg caagccaagt tcaaaaactg
     7081 ttcctgcacc ggcctgcccg cctgtcgtga tgccaaaaac gttcgtgccg gataaggcca
     7141 ggcttccgac gggattcgct ccgtccaccg cgaaattttg cactatttga taatctgtcc
     7201 cgtcagtgtt gatggtaaat aatgtgccca cgctattgct gccaccggcg gaagttgtcc
     7261 cgcagagcgt gttgccggaa agcgccagcg tgcattgcgg catgttgcca tctggcaccg
     7321 ggccaaacgc ataaagttga ctgtaattgt tgccatcggt cccgattgcg aacaccgtgc
     7381 ccaagttatt gctcccgcca acttccgtgg ttccatacaa tgtgttgccc gaaagcacta
     7441 aattacctac tggagtccct ccggtggccg cggcaaagtg atgtaacaca ttgaaattgc
     7501 taccgttggt ggaaatcgag taaatgacac catacccgtt cgaaccacct tgcaccgtca
     7561 taccatagaa agtgct
//
LOCUS       DASZMM010000461         6314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4870878, whole genome shotgun sequence.
ACCESSION   DASZMM010000461 DASZMM010000000
VERSION     DASZMM010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4870878"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..252)
                     /locus_tag="VG754_11065"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="VG754_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            446..2767
                     /locus_tag="VG754_11070"
     CDS             446..2767
                     /locus_tag="VG754_11070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HWF19802.1"
                     /translation="MNGDDETRAAQNPFARGQKIAGGRFTLLRPIGRGGMGEVWLAQD
                     ERLHESVALKFLPRSIRGDATSLDDLLRETARSHKLSHPNIVRIHDLHEDGLLAFIAM
                     EYVDGSTLASVRLQSSDRLLRWDYLEPLVVQLCGALEYAHGQKIIHRDLKPSNIMVDS
                     RGNVKLADFGIAATVTDTMSRVSLRHATSGTALYMSPQQMNGEKPRPTDDIYAMGATL
                     YELLSSKPPFYTGDISHQVRNITVPSLEQRLAELEVTNDVPPNVSALIVSCLAKDAIA
                     RPQSAAAFLEQLQNPAPETRFTQTQESNASAETLTAMPSGARTTARASDETRAKKTPV
                     SPSAPHVIDVLEDKNNRARSGLKLALVCGVVLLFVAFIWLALKSAEPKPAASEDNASP
                     NNSASNNDLPTNLQQSPLVTSNSSPLPASNAAITLVSQPDTNVVVNPVTNAAIKPQTN
                     VQESPVKPAPPPPVITVAPEPPKEIIQPKVQPETVQSKPPVPTNAPPELAQTKISPEP
                     PQPKPASAPAPRPPPPVATPQTTFVPERKPITPTVPQSDRYWVNTLGMRFVPVPGTAV
                     LFAVTDTRVQDYQAFVNATQRPWTKPDFQQTSTHPAVNISWEDAMLFCEWLTEKERAE
                     GKLGPDQSYRLPTDAEWSIAAGLETESGGTPLDKDGRIAGVYPWGNQWPPPPGSGNFS
                     RALKADVFDYTSPVGSFKPNRHGLFDMGGNVRQWCDDWYANSQHTRVLRGSSWTTVFQ
                     ESLLSSCRNEAVPQQVSEFNGFRCVLVPNAASQ"
     gene            complement(2965..3447)
                     /locus_tag="VG754_11075"
     CDS             complement(2965..3447)
                     /locus_tag="VG754_11075"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HWF19803.1"
                     /translation="MNEPTQQHHDDDLAQASHEDILAALFANMVIQQTNMAMMLLGKV
                     AHPETGRIIKDVEAAKMFIDQLEMLEAKTKGNLSKQEEGLLKQALAALHMAFVEAVDS
                     GGAAATAGDGGAHQEAPPERGTTPKPAPAPEAPAAEKPADITAGTATDDSRKRFSKKY
                     "
     gene            complement(3444..4115)
                     /locus_tag="VG754_11080"
     CDS             complement(3444..4115)
                     /locus_tag="VG754_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWF19804.1"
                     /translation="MKLPSEVPVMTLPNATLFPQALLPLYIFEPRYRQMLVDSLHSDR
                     MFSVAMQKPGRTRETPSPVAGLGLIRVSVGHQDGTSHLVLQGIARVELVETVRYKPYR
                     IQRIRPLSSPPCNDVVVDALLAKVRELLEERLSLGLGFPPPIAVNKSKAASMPHISAK
                     DVLQYLDKLTDPEQVADLVSCAVLSGPVERQTILEAVNLETRLKTLIHFLMAEIARQR
                     KDKNK"
     gene            4157..4390
                     /locus_tag="VG754_11085"
     CDS             4157..4390
                     /locus_tag="VG754_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19805.1"
                     /translation="MEMVAAASQTGLATIASPAAATAGQRVMLLIPAAVASQFFKIFA
                     RIDFVQINLYQKIKVIDKSSLLIDGVTRIFVLL"
     gene            4387..5187
                     /locus_tag="VG754_11090"
     CDS             4387..5187
                     /locus_tag="VG754_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19806.1"
                     /translation="MKTKVAIVILALIAIGLGIGLISFKDQASKQHDDDVAKYLVLSN
                     ELTTTTTKWEEEKQVHLSIQQELDAKKDDMAKLSNTLSQTTVNLEKTQAALEAEQKEV
                     AARDAKIAQLETQNATLDKQAGDLKSSINNLETQIADTQKKLDASEGDKAFLTKELNR
                     LMAEKSELERQFSDLAVLRAQVHKIKTELATARRLDWIRRGIFANAEEKGAERLMQHA
                     SATTTVASARPAVATNMDLNVEVNSDGSVKVIPPLTNPPPAINPPSQQ"
     gene            5184..6242
                     /gene="moaA"
                     /locus_tag="VG754_11095"
     CDS             5184..6242
                     /gene="moaA"
                     /locus_tag="VG754_11095"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWF19807.1"
                     /translation="MNRDRLLVDAHGRVLRDLRVSITDRCNFRCLYCLPETEAAQNFY
                     RGRWATLPNPKPIAHQWQPRSKILSFEEIERVVRIAVGLGIQKIRLTGGEPLLRRDAE
                     QLVARIAAIPGITDLALTSNGFLFQEKGRALRDAGLRRISFSLDSLDRANFQKITGRD
                     GLDEVLGSIRLARELGMQPVKVNAVVIRGINDHEIEALAEFARNEDLAMRFIEFMPLD
                     SARAWLKDLVVTGREILHRLQARNALQPVVSGNASETAKRWKFPDGRGEIGLISPVSE
                     PFCGHCNRVRLTADGKIRTCLFSVVEHDLRTRLREGDSDEAIAEYLQSVVWQKEERHH
                     IGEPEFVAPERSMSCIGG"
BASE COUNT         1407 a         1776 c         1797 g         1334 t
ORIGIN      
        1 gtcgagcacg gcgtcgaagt ccttatgtgc ggcgggagct tcatcctttg gatattgacg
       61 gcaattcgtg aggatatcgt ttgccacaaa ttcatcatca acagttttct ggtcgagcgc
      121 tcggtccgcg gctttgcggc ccatgcaacg gcctgcaccg tgattcacgg aataggcgct
      181 cttttctgcg cccgggtcgg cgaccatgat gacgcttcca gcggtcgggt tgccgggcaa
      241 caggattgga tgcgcgcgct atgtgccctc ctcaatccgt ttagcgagtt ccggatacaa
      301 gcggaaggca ttcgcgtacg agatgcttcg caaatccgct gggttgcggg gactttccag
      361 gccccgctcc agaacagtgt ccagatacac aactttctcc cgctgttgtt atgggccgga
      421 ttttctgtta ctttcggcaa cgctcatgaa tggtgacgat gaaacgcggg ccgcgcaaaa
      481 tccttttgcg cgcggccaga aaattgccgg cgggcggttc acgttgctgc gcccgatcgg
      541 ccgcggcggc atgggcgaag tctggctcgc gcaggacgag cggttgcacg aatcggttgc
      601 gctcaaattt ctcccgcgct ccatccgcgg cgacgccacc tcgctcgatg atctcctgcg
      661 cgaaacggcc cgcagccaca aactcagcca tccgaacatc gtccgcatcc atgaccttca
      721 cgaggatggc ctgctggcct tcatcgccat ggaatatgtg gatggttcga cgcttgcctc
      781 ggtgcggctt caatcttcgg ataggttgtt gcgttgggat tacctggagc cgctcgtcgt
      841 gcaattatgc ggcgcgctcg aatacgcgca cgggcaaaaa atcatccatc gcgatctcaa
      901 gccttccaac atcatggtgg acagccgtgg gaatgtgaaa ctcgccgatt tcggcatcgc
      961 ggcaacagtg accgacacga tgagccgcgt ttcgctgcgt cacgccacga gcgggaccgc
     1021 gctctacatg agtccgcaac agatgaacgg cgaaaaaccg cgtcccaccg atgacattta
     1081 cgccatgggc gcgacgctct acgaattgct cagcagcaag ccgccttttt ataccgggga
     1141 catttcgcat caggtgcgca atatcactgt gccttcactc gaacagcgcc ttgccgaact
     1201 cgaagtcacg aatgacgttc cgccgaacgt cagcgcgctg atcgtttcct gtcttgccaa
     1261 ggatgccatc gcgcgtccgc agagcgccgc ggcttttctc gaacaattgc aaaatccggc
     1321 gcccgagacg cgattcaccc agactcaaga aagcaatgcg tccgctgaga cattgaccgc
     1381 gatgccttcc ggtgcgcgga caaccgcgcg cgcttctgat gaaacgcgcg caaagaaaac
     1441 tcccgttagt ccaagcgcgc ctcacgtgat tgacgttttg gaagacaaaa acaaccgtgc
     1501 ccgatctggt ctgaagctgg cgctggtctg cggtgttgtg ctcctgtttg ttgcctttat
     1561 ctggcttgcg ctcaaaagcg ccgaaccaaa acccgcagct tcggaggaca atgcatcgcc
     1621 caacaattcg gcatccaaca atgatttgcc gaccaacctt caacaatcgc ctttggtcac
     1681 atccaatagc tctccgctcc ccgcgagcaa cgcggccatc acacttgtat cgcaacccga
     1741 cacgaacgtc gttgtaaatc cggttacgaa tgctgccata aaacctcaaa caaacgtcca
     1801 agaatcgccg gtcaagccag ccccgccacc acctgttatc accgtagcgc cagaacctcc
     1861 aaaggaaatt attcagccga aggttcagcc cgagacggtg caatccaagc ctccagttcc
     1921 aaccaacgcg ccgccggaat tggcacaaac caaaatttca ccggaaccgc cgcaacccaa
     1981 gccggcatcc gcgcctgcgc cacggccgcc acctccggtc gcgactccgc aaactacgtt
     2041 cgttccggaa cgcaaaccca tcacgccaac agtcccgcaa tcagaccgct attgggtgaa
     2101 tacgttggga atgcgattcg ttccggtgcc gggaactgcg gtgctgttcg cggtgaccga
     2161 cacccgtgtg caggattatc aagcgttcgt gaatgcgacg cagcggccat ggaccaaacc
     2221 cgatttccag cagacctcga cgcatccggc ggtaaatatt tcatgggagg acgcgatgtt
     2281 gttttgcgaa tggcttaccg agaaggaacg cgctgaagga aagcttgggc cggaccagag
     2341 ttatcggctg cccacggatg cggaatggag cattgcggca ggattggaaa cggaatcggg
     2401 cgggacgccg ctcgataaag acggacgcat agcgggtgtt tatccttggg ggaaccagtg
     2461 gccgccgccg ccaggctctg gaaatttcag tcgcgcgttg aaggccgacg ttttcgatta
     2521 cacttcgccg gtcgggagtt tcaagccgaa ccgccatggt ttgttcgaca tgggcggcaa
     2581 cgtgcggcaa tggtgcgacg attggtatgc gaacagccag cacacgcgcg tcctgcgcgg
     2641 cagttcgtgg acgacggtgt tccaggaatc attgctttct tcctgccgca atgaagcggt
     2701 gccgcagcaa gtttccgaat tcaacggctt tcggtgtgtg ctcgttccga acgctgcttc
     2761 gcaataaaaa ggaagctttg cgggaaagcg ctttgactta tctaagccat tcagaaatgg
     2821 aaaacggcgc gcaactgtgt cctcagaacc agtagcagcc tggaacagtt gggatttcag
     2881 tagcttgctg cggctggcgc tttgctcaca gccgcgcccc attatttatg agacaagctt
     2941 gtaataaata ccgcagaagc ggattcaata tttcttgctg aaacgtttcc gcgaatcatc
     3001 cgtcgctgtg ccagcggtaa tgtcggccgg tttttcagca gcgggagctt cgggagcggg
     3061 ggctggcttg ggtgtggtgc cgcgttcggg gggcgcttcc tgatgcgcgc cgccatcgcc
     3121 agccgttgcg gctgcgccgc cggaatccac agcttcgaca aacgccatgt gcaacgccgc
     3181 aagcgcctgt ttgagcaggc cttcctcctg tttggaaaga ttgccctttg ttttggcttc
     3241 gagcatttcc agttggtcaa tgaacatctt tgccgcctcc acatccttga tgattcgtcc
     3301 ggtttcgggg tgggcgactt tgccgagcag catcatggcc atgttggttt gctggatgac
     3361 catgttggcg aacagggcgg ccaggatgtc ttcgtgactg gcctgggcca gatcatcgtc
     3421 gtgatgctgc tgagttggtt cgttcatttg ttcttatcct ttcgttgacg ggcgatttcc
     3481 gccatcaaaa aatgaatgag cgtcttgagg cgggtttcca agtttacggc ttccaaaatg
     3541 gtctggcgct cgaccgggcc gctcaatacg gcgcacgaaa ccagatcggc gacctgttcg
     3601 ggatcagtca gcttgtcgag atattgcagc acgtccttgg ccgaaatgtg cggcatggac
     3661 gcggccttcg atttgttcac cgcgatgggc ggaggaaaac caagaccgag cgacaaacgt
     3721 tcttcgagca attcgcgcac cttggccagc aaagcgtcca ccaccacgtc gttgcatggc
     3781 ggcgatgaaa gcggccggat gcgctggatg cgatacggtt tgtagcgcac tgtttcgaca
     3841 agttcaacgc gggcaattcc ttgcaggact agatgggaag ttccgtcctg gtgtcccacc
     3901 gagacgcgga tcaaacccaa acccgcgacg ggcgaaggtg tttcacgcgt gcggccgggc
     3961 ttttgcatcg cgacggaaaa cattcggtca gaatggaggg aatccacgag catctgccta
     4021 tagcgcggct cgaaaatata gagcggcagc aaggcctgcg gaaagagcgt cgcattgggc
     4081 agggtcatga cgggaacttc actcggcaac ttcatgaata gaatttaggc gagaccgtca
     4141 tttaatcaag gcgcagttgg aaatggttgc ggcagcgtct caaaccggac tggcgactat
     4201 cgcaagccct gctgcggcga cagcgggaca aagggttatg ttgctgattc cagcggcggt
     4261 tgcatcgcaa ttttttaaaa tttttgcgcg catcgatttt gttcaaataa atttgtatca
     4321 aaaaataaaa gttattgata agtcaagctt gctaattgac ggtgtaacac gcatctttgt
     4381 gctcttatga agacaaaagt cgctatcgtg atattggcgc tcatcgccat cggattgggc
     4441 attggtttga tttcgttcaa agaccaggcc tcaaagcagc atgacgatga tgtggcgaaa
     4501 tatctggttc tttccaatga attgaccaca acgactacga aatgggagga agaaaagcag
     4561 gtgcatcttt cgattcaaca ggaactcgac gccaaaaagg acgacatggc caagctttcc
     4621 aatacgctga gccagaccac cgtaaacctg gaaaagacac aggctgccct cgaagccgag
     4681 caaaaggaag ttgccgcgcg cgatgcgaag attgcccagc ttgaaacgca aaatgcgact
     4741 ctcgacaagc aggccgggga tttgaagtct tccatcaaca atcttgaaac gcagatcgcc
     4801 gatacgcaaa agaaattgga tgcttccgag ggcgacaagg cgtttctcac caaggaactc
     4861 aaccgtttga tggctgagaa atccgaattg gaacgccagt tcagcgacct tgccgtgctt
     4921 cgcgcgcaag tgcacaagat caagacagaa ctcgcgacgg cccgccgtct tgattggatc
     4981 cgccgcggca ttttcgccaa tgcggaagaa aagggcgcgg aacgactcat gcaacatgcc
     5041 tccgccacca cgaccgtggc gagcgccagg ccggcagttg cgaccaacat ggatttgaac
     5101 gtggaagtca attccgacgg gagcgtgaaa gtgatccctc cgctcaccaa cccgccgcct
     5161 gcgataaatc cgcccagcca gcaatgaacc gcgaccgcct gctcgtggac gcgcacgggc
     5221 gcgtcttgcg cgacctgcgg gtgagcatca ccgaccggtg caatttccgt tgtctctatt
     5281 gcctgccgga aacggaggcg gcgcaaaatt tttatcgcgg acgctgggcc acgcttccca
     5341 atcccaaacc catcgcgcat caatggcagc cgcgttccaa gattctatcg ttcgaggaaa
     5401 ttgagcgcgt ggtgcgtatc gcggtcgggc ttggcatcca aaaaatccgg ctcaccggcg
     5461 gagaaccgct tcttcgacgc gatgccgaac aactcgtggc ccgcatcgcc gcgattcccg
     5521 gcatcaccga ccttgcgttg accagcaacg gttttttgtt ccaggaaaaa ggacgcgcat
     5581 tgcgggatgc gggtttgcgg cgcatcagct tcagcctcga ttcgcttgac cgcgcgaatt
     5641 ttcagaaaat caccggacgc gacggtttgg atgaagtgct tggcagcatt cgattggcgc
     5701 gtgaattggg gatgcagccg gtgaaggtca acgcggtcgt catccgcgga atcaacgatc
     5761 acgaaatcga ggcgctggcg gaatttgcgc gcaacgaaga tttggcgatg cgtttcatcg
     5821 agttcatgcc gctcgattcc gcgcgtgcgt ggctgaaaga ccttgtcgtg accgggcgcg
     5881 aaattcttca tcgtttgcag gcacgtaatg cgcttcaacc cgttgtctcc ggcaacgcgt
     5941 ccgagacggc aaagcgttgg aagtttcccg atggccgcgg ggagatcggt ttgatatcgc
     6001 cggtgagcga accgttttgc ggccattgca atcgcgtgcg actcaccgcc gatggcaaaa
     6061 tccgcacctg tttgttcagt gttgtggagc acgatttgcg gacgcgtctg cgcgaaggcg
     6121 attcggatga agcgatcgcg gaatatttgc aatccgtcgt ctggcagaaa gaagagcggc
     6181 atcacatcgg cgagccggaa tttgtcgcgc ccgaacgcag catgagttgc attgggggat
     6241 gaaaaggctt gaaaaacggc ctttctgaag tgaaagttgc atcatgagcg cagccatcat
     6301 gcgggaaaaa cggc
//
LOCUS       DASZMM010000462         4130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1006150, whole genome shotgun sequence.
ACCESSION   DASZMM010000462 DASZMM010000000
VERSION     DASZMM010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006150"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /gene="xseA"
                     /locus_tag="VG754_11100"
     CDS             complement(<1..1100)
                     /gene="xseA"
                     /locus_tag="VG754_11100"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWF19808.1"
                     /translation="MTKPSNSQWDFGELFPVEATRKVLTVAELTAQVRRLLEKNVGQV
                     WVTGEVTNFRAQSSGHFYFTLKDAESQLNCVLFRNEALESRALLQDGRKVVLQGDLTV
                     YEPRGQYQLRVTAVELQGIGALQVAFEKLKQKLSAEGLFAVERKRPLPRYPQRIGIVA
                     SPTGAAIRDVLHVVRRRNPGLEMVLAPCRVQGQGAGDEIAAAIRLLNRFSKRETSNIE
                     RRTSNAERKRPFTHLGEEATDVSTSDSALRVPHSALELILVTRGGGSLEDLWAFNEEV
                     VARAIFESELPVVSAVGHEIDFTISDFVADLRAATPSAAAEIITEGVYSSCQFVSEAA
                     ERMSQLVRQRIADKEYELVQTSQRLLRMHPRR"
     gene            complement(1097..2083)
                     /locus_tag="VG754_11105"
     CDS             complement(1097..2083)
                     /locus_tag="VG754_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19809.1"
                     /translation="MKKVLFITFVFLAIAGMALVFLLRKPSGPPIPSYQGKSADVWLS
                     EVFTTNQAQALSAFSEMGTNAWPFLVDVFRQRESSLNNFSSHVYPKLPWFFKKYVKRP
                     ILAEEKWNSAELVFLQHPHERDLTPTLLEMLRDKSNQAHGYVVGLLMRDAAGHGDWVP
                     LLIPCLHEKQIATREETTMMLGQLGPDAKAAIPDLSAGLHDTNEMLRVESAFALWRID
                     HQTNGVAAIQNALLTSADVLMKRESAILLSQIGWTDPSLIPVYAKLVTDSDKALSVSA
                     AIMLENFGTNAKSAVPSLVKAYELNPPYDRQYIWSALRAVDPNAAAKCKSGK"
     gene            complement(2085..2885)
                     /locus_tag="VG754_11110"
     CDS             complement(2085..2885)
                     /locus_tag="VG754_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HWF19810.1"
                     /translation="MKILFIGDIVGEPGRRAVKELLPRLRRQLEADIVIANGENAAGG
                     SGITPATAQEIFGAGVDIMTTGDHLWDQKEVIGLLQNESRFLRPLNYPQGTPGQGSVV
                     YQANNQPPVGVINLQGRTFMPDLDNPFTTVRAEVERMRQKTNIIFVDFHAEATSEKAA
                     IGRMLDGQVSAVIGTHTHVQTADEQIFPGGTAFLSDAGFTGPHESVIGREIEPVIKRF
                     LTLQPQRFAVAEKDIKLQGALVDINGHTGRAEKIQRISEPLADAGKKG"
     gene            complement(2933..3238)
                     /locus_tag="VG754_11115"
     CDS             complement(2933..3238)
                     /locus_tag="VG754_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19811.1"
                     /translation="MKTCPNCGRENAAGTNWCPECGHSDKFYVFFGRLFAISRIMRRW
                     CLVGFLGVVLIAIGIYKKLLWLKIAGFVLAVPMIWVYFVVLFIFLPAMGLKKLRKWF"
     gene            complement(3278..3577)
                     /locus_tag="VG754_11120"
     CDS             complement(3278..3577)
                     /locus_tag="VG754_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19812.1"
                     /translation="MVLFQAQQDRATELRIASNDKNGAAMKYKINETWHDFAPPPAHI
                     LPGVIEELLRLANIQEAAFPKRGVINQTVHGYLMRWQITMMSPDAECVLTRIEHE"
     gene            complement(3614..>4130)
                     /locus_tag="VG754_11125"
     CDS             complement(3614..>4130)
                     /locus_tag="VG754_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWF19813.1"
                     /translation="WRNGGSVSETEKKEPQVKVEYVTPGMQKLLEVCRAKGAKNVAIA
                     GGLDWAYDLRGVASGHALNDASGNGVVYDSHFYPMKKWFTHGDTKSQDWDRIVLSGGG
                     KYPVLIGEFGDGKDNYESKVIEFAKEHDLPWTAWCLHPGAKPNLIKDWNYTTTDYGAV
                     VKEALQAASSR"
BASE COUNT          923 a         1243 c          998 g          966 t
ORIGIN      
        1 ctgcggcgcg gatgcatccg caacaggcgt tggctcgttt ggaccagttc atattccttg
       61 tcggcgatgc gctgtcgcac caattggctc atgcgttcag cagcttctga cacgaactgg
      121 caactcgaat agactccttc ggtgatgatt tccgccgcgg cgctgggcgt cgcggcgcgc
      181 aaatccgcga cgaaatcgct gatggtgaaa tcaatctcat gtcccaccgc cgagaccacc
      241 ggcaattcgg attcgaaaat cgcccgcgcc acaacttcct cgttgaacgc ccacaaatcc
      301 tccaggcttc cgccgccgcg agtcaccaaa atcaactcaa gtgcggagtg cggaacgcgg
      361 agtgcggaat cagacgttga aacgtcagtt gcttcttctc ccagatgggt gaaggggcgt
      421 tttcgttcag cgttcgatgt tcggcgttcg atgttcgatg tttccctttt agaaaacctg
      481 ttcagcaatc gaatcgccgc cgcgatttcg tctcccgccc cctgcccttg cacacggcat
      541 ggcgcaagca ccatctccaa acccggattt cgcctccgca ccacatgcaa cacatcgcgg
      601 atcgccgcgc ctgtcggcga tgccacgatg ccgattcgct gcggataccg cggcaaagga
      661 cgcttgcgtt cgacggcgaa caatccttcc gcgctcaact tctgcttcaa tttttcaaac
      721 gccacctgca acgcgccgat gccctgcaac tccaccgcgg tgacgcgcaa ctgatattgc
      781 ccgcgcggtt catacaccgt gaggtcgcct tgcagcacca ccttgcggcc atcctggagc
      841 agggcgcgac tttccaacgc ttcgttgcgg aacaagacgc aattcaactg cgattccgcg
      901 tccttgagcg tgaaatagaa atgaccggaa ctttgcgcgc gaaaattcgt cacctcgccc
      961 gtgacccaaa cctgcccgac gttcttctcc agcaaccgcc gcacctgcgc ggtgagttcc
     1021 gcgacagtga gcaccttgcg cgtcgcctcc accggaaaca actcgccgaa atcccattgt
     1081 gaatttgagg gtttggtcat tttcctgatt tgcacttcgc cgccgcattt ggatcaactg
     1141 cccttaatgc actccatata tattgccgat catacggcgg gttcagttca taagctttca
     1201 cgagtgacgg cactgccgat ttcgcattcg tgccaaaatt ttcaagcatg attgccgcgg
     1261 agacagacag cgccttgtcg gaatcagtca ccagcttggc atacactgga atcagcgaag
     1321 gatctgtcca cccgatttgt gaaagcagga tggctgactc tcgtttcatt aaaacgtcag
     1381 cagatgtcag gagcgcgttt tggatggcgg cgacgccatt agtttgatga tcaatcctcc
     1441 aaagtgcaaa tgccgattcc acgcgtaaca tttcgtttgt gtcatgaagt cccgcgctca
     1501 agtcgggtat cgccgccttg gcatcaggtc cgagttgtcc caacatcatc gttgtctctt
     1561 cgcgggtcgc tatttgcttt tcgtgaaggc atggaatcaa caacggaacc caatcgccat
     1621 gtcccgctgc atcccgcatc aaaaggccaa cgacataacc atgggcttgg ttgcttttat
     1681 ccctaagcat ttcaagcaaa gtcggcgtca agtccctttc atgtggatgt tgcaaaaata
     1741 ctaattccgc cgaattccat ttttcctccg ccaatatcgg tcgcttgaca tattttttga
     1801 aaaaccacgg caactttggg taaacatggg agctaaagtt gtttagcgag gactcgcgct
     1861 gacgaaaaac atcaacgagg aacggccagg cattggttcc catctcggaa aaagcgctca
     1921 acgcttgagc ttgatttgtg gtgaacactt cggagagcca aacgtccgca gatttgcctt
     1981 ggtaagaggg aatgggaggt cccgacggct ttcttaagag gaacaccaac gccatacccg
     2041 ctatcgccaa aaagacaaac gtgatgaaaa ggaccttttt caattcatcc tttcttcccc
     2101 gcatccgcca acggctccga aatccgttga atcttctccg cgcggcctgt gtgcccattg
     2161 atgtccacca gcgcgccttg caacttgatg tctttttcgg cgacggcgaa acgctgcggt
     2221 tgcaaggtca aaaagcgttt gatgaccggc tcgatttccc gtccgatgac gctttcgtgc
     2281 ggcccggtga aaccagcgtc gctcaggaac gccgtgccgc ctggaaaaat ctgttcatcc
     2341 gccgtttgca catgcgtgtg cgtgccgatg accgcgctca cctggccgtc gagcatccgc
     2401 ccgatggcag ctttttcgga agtcgcctcg gcgtggaaat ccacgaagat gatgttcgtc
     2461 ttctgccgca tccgttccac ctcggcgcgc acggtggtga aaggattgtc gaggtccggc
     2521 atgaacgtgc gtccttgcaa attgatgacg ccaaccggcg gctggttgtt cgcctgatag
     2581 accacgctcc cctgccccgg cgttccctgc ggataattca atggccgcaa aaaccgcgat
     2641 tcattctgca acaacccgat gacctccttc tggtcccaaa gatgatcgcc ggtcgtcatg
     2701 atatcgacgc ccgcgccgaa aatttcctgc gcggtcgccg gcgtgatgcc cgaaccgccc
     2761 gctgcatttt cgccgttggc gatcacaatg tccgcctcca attggcggcg caagcgcggc
     2821 aacaactcct tcaccgcccg acgccccggt tcccccacga tgtccccgat gaaaagaatt
     2881 ttcacggctt caaggtaccg tcagccgacg ctctggcaac tggaaaaaag gatcaaaacc
     2941 atttgcgaag cttcttcaaa cccattgctg gtaaaaaaat aaacaggacc acaaaataaa
     3001 cccaaatcat gggtactgcc aaaacgaagc ccgcgatttt cagccataag agtttcttgt
     3061 aaataccgat tgcaatcagc acaacgccca ggaagccgac aagacaccag cgccgcatga
     3121 tccggctaat cgcaaataat cgtccaaaga aaacatagaa tttatctgaa tgcccgcatt
     3181 ccggacacca attggtgccg gcagcatttt ctcgtccgca attcgggcat gtcttcatct
     3241 attagcaaat tgcgtaggcg aactttttaa tgaattctca ttcatgctcg atacgtgtaa
     3301 gcacacactc ggcatcggga ctcatcatcg tgatttgcca tcgcatgaga taaccgtgca
     3361 cagtctggtt gatgacgcct ctcttgggaa atgccgcttc ttgaatgttt gccaatcgta
     3421 aaagctcctc tatcacacca ggcagaatgt gcgcgggagg tggcgcaaaa tcatgccatg
     3481 tttcatttat tttatatttc attgcggcgc cgttcttgtc gttggaagca atcctcaatt
     3541 cagtcgcacg atcttgttgt gcctggaaca aaaccaaaga tgtgaatcgt ttgatgggtt
     3601 cgttggccat aagtcacctg cttgacgcag cctgcaacgc ctccttcacc accgcgccat
     3661 aatccgttgt tgtataattc caatctttga tcaagttggg ttttgcgccc ggatgcaggc
     3721 accacgcggt ccaaggcaaa tcgtgctctt tcgcgaactc aatgactttg ctctcgtaat
     3781 tgtccttgcc gtcgccgaat tcgccgatga gcaccggata tttgccgccg ccggaaagca
     3841 cgatgcggtc ccaatcctgc gacttcgtat cgccgtgcgt gaaccacttc ttcatgggat
     3901 aaaaatgcga atcgtaaacc acgccgttgc cgctcgcgtc gttcaacgcg tgcccgctgg
     3961 caactccccg caaatcgtaa gcccaatcca aaccgccggc gatggccaca ttcttcgcgc
     4021 ccttcgcgcg gcaaacctcc agaagtttct gcatgccggg cgtgacatat tccactttca
     4081 cctgtggttc cttcttctcg gtctcgctca cgctcccgcc gttgcgccaa
//
LOCUS       DASZMM010000463         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2117879, whole genome shotgun sequence.
ACCESSION   DASZMM010000463 DASZMM010000000
VERSION     DASZMM010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117879"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2540)
                     /locus_tag="VG754_11130"
     CDS             complement(<1..2540)
                     /locus_tag="VG754_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF19814.1"
                     /translation="MNPSRPFILRPVATSLLMAAILLVGVVAYRQLPVSALPEVDFPT
                     IQVITFYPGASPDVMASAVTAPLERQFGQVAGLNQMTSVSSGGSSVITLQFALYLNID
                     VAEQEVQAAINAAQTYLPIDLPTPPVYSKSNPADAPVLTLALTSKAMPLSKVEDLADT
                     RLAQKISQVSGVGLVTIVGGQKPAVRIQANPTALSAYGLSLEELRASLQQASVNQAKG
                     NFDGPKTAYQIGANDQLTTSMDYTDLVVAYRNGSPVMLKDVANAFDGVENTLQAAWVN
                     QTPAVIINVQRQPGANIIQVVDRITQRLPQLKATMPPSVDLSVVTDRTATIRASVRDV
                     EFELLLTIALVVLVIFLFLRTVSATIIPSVAVPLSLVGTFAVMYMLGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENIVRYIEQGEKPLDAALKGAEQIGFTIMSLTVSLVAVLIPLL
                     FMQDIVGRLFREFALTLSVTILISAVVSLTLTPMMCARLLHRTDEKKQGRIARASERA
                     FERTIEHYGRSLRWVIDHRAVTLLVAIATLVLTVVLYILVPKGFFPVQDTGVIQGISE
                     ANQTASFPAMAARQQALANAVLQDPNVESLSSFIGADGINTTLNSGRMLINLKPLSQR
                     SLKASQIIQQLQPAIANVDDIVLYMQPVQDLTVEDRVSRTQYQYTLEAPDADLLSMWT
                     GRLMERLRQIPELRDLATDQLVEGQQAQLVIDRVTAARLGITAQQIDETLYDAFGQRQ
                     VSTLFTQLNQYHLVLETLPPFQKSPLKLNDIYIQATNGGAVPLSAIAHVETKSAPLSI
                     NHQGQFPVVTISFNLAPGASLGAATKAIDNAANELKMPASVQTAFQGAA"
     gene            complement(2537..>3780)
                     /locus_tag="VG754_11135"
     CDS             complement(2537..>3780)
                     /locus_tag="VG754_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF19815.1"
                     /translation="KRRHGWIWLIIFLVLVVVGVIFFLRRHKPKPAPPPPIPISTTNI
                     QLGDIAVSVTALGSVLPVYTVGISPRVDGQLIKVAYTEGQLVKTNDLLAVIDPGPFEA
                     LEIEAEGQLARDQAQLDIARIDLKRFQAAYERKAIPEQQLADQQGVVHQDEGTVKFDQ
                     GQLANARVQLAYCYIRAPISGRVGLRLVDPGNVVHAANTNAMLAIAQLQPITVLFSVA
                     EDFLPQIQRQVQAGHSMLVEAYDRTESKKIATGEYLTMDNLIDTATGTIRIKATFNNE
                     DFALFPNQFVNARLVIEVLTNQLLIPTFAIQRNPQGAFVYVVTNKVANEVVTTNHNTF
                     TNMVTNEVVTMQNIKVGVSDTNSTAVEGVETGQLIAIDNFNKLGEGVKVKALKPGESP
                     STNAAPAGPRNSKGKTNAPSK"
BASE COUNT          805 a         1049 c         1097 g          829 t
ORIGIN      
        1 gccgcgcctt gaaacgcggt ttgaacggag gccggcattt tcaattcgtt cgcggcattg
       61 tcgatggctt tggtcgccgc gcccaaggac gctcctggcg cgagattgaa tgagatggtc
      121 acgacgggaa attgcccctg atgattgatg gacaacgggg ccgatttggt ttcaacgtgc
      181 gcaatggcgc tcaacggcac cgcgccgccg ttggtcgctt ggatgtaaat gtcgttcaat
      241 ttgagcggac ttttttgaaa cggcggcaac gtctccagca cgagatggta ttgattcaat
      301 tgcgtgaaga gtgtggagac ttgccgctgg ccgaaagcgt cgtaaagcgt ttcgtctatc
      361 tgttgcgcgg taatgcccag gcgcgccgcg gtgacgcggt caatcaccaa ctgcgcctgc
      421 tggccctcca ctaattggtc ggtcgccaaa tcgcgcaatt ccggaatctg gcgcagccgt
      481 tccatcagcc ggcccgtcca cattgagagc aggtccgcat ccggcgcttc gagcgtgtat
      541 tgatattgcg tccggctgac gcggtcttcg accgtcaaat cctgcaccgg ctgcatgtac
      601 aaaacgatgt catcaacatt tgcgatggcg ggttgcaact gctggatgat ttgcgaggcc
      661 ttgaggctgc gttgggaaag cggcttgaga ttgatgagca tccgcccgct gttgagcgtg
      721 gtgttgatgc cgtccgcccc gatgaatgag gaaagacttt cgacgtttgg gtcttgaaga
      781 acggcgttcg ccaatgcctg ttgccgcgcg gccatcgcgg ggaaagacgc agtttggttc
      841 gcttcagaaa ttccctggat gacgccggtg tcctgcaccg ggaaaaaacc ttttggaacc
      901 aaaatgtaaa ggacgaccgt caagacgagc gtcgcgatcg ccacaagcag cgtcaccgcg
      961 cgatggtcaa tcacccagcg caagctccgg ccataatgtt caatggtccg ctcaaacgcg
     1021 cgttccgagg cgcgcgcgat ccgcccctgt tttttttcat ccgttcgatg cagcaatcgc
     1081 gcgcacatca tcggcgtcaa cgtcagcgac acgaccgccg agatgaggat ggtgacgctc
     1141 aaggtgagcg cgaattcccg gaacagccgc ccaacgatgt cttgcatgaa caaaagcgga
     1201 atcaagaccg cgaccagcga aaccgtcaaa gacatgatcg tgaaaccgat ttgttccgcg
     1261 cctttcaatg ccgcatcgag cggtttctcc ccttgctcga tgtaacgcac gatgttttcg
     1321 atcatcacga tggcgtcatc caccacgaag ccagtcgaga tggtgagcgc catcagcgtg
     1381 agattgttca ggctgaatcc cagcatatac atgactgcga aagtgccgac gagggagagc
     1441 gggactgcaa cgctcggaat gatggttgcg gaaaccgtgc gcaaaaacaa aaagatgacc
     1501 agcacaacca gcgcgatggt gaggagcaat tcgaattcaa cgtcgcgcac cgaagcgcga
     1561 atggttgcgg tccggtccgt gacaacggaa agatcaaccg atggcggcat cgttgctttg
     1621 agttgcggca ggcgttgcgt gatgcggtca accacctgga tgatgtttgc gcccggttgc
     1681 cgttgcacgt tgatgatgac ggcgggcgtt tggttgaccc acgccgcttg caaagtattt
     1741 tccacgccat cgaacgcatt ggccacgtcc ttgagcatga ccggcgagcc attgcggtaa
     1801 gccacgacaa ggtcggtgta atccatgctg gtcgtaagct ggtcattcgc gccgatttga
     1861 taagccgttt ttggcccgtc gaaatttccc ttcgcctggt tgacgctggc ttgttgcaat
     1921 gaggcgcgaa gttcttcgag ggaaagcccg taagcagaca acgcggtcgg gtttgcctgg
     1981 atgcgcacgg cgggcttctg gccgcccacg atggtcacca gacccacgcc gctgacctgc
     2041 gaaatttttt gggccagacg cgtgtcggcc aaatcttcga ccttggaaag cggcatcgcc
     2101 ttgcttgtca acgccagcgt gagaacgggc gcatcggcgg gatttgattt gctatagacc
     2161 ggaggagtgg ggagatcgat cggcagataa gtttgcgccg cattgatcgc cgcctgcact
     2221 tcctgttcgg cgacatcaat gttcaggtaa agcgcgaatt gcagcgtgat gaccgaactg
     2281 ccgccggagc tgaccgaagt catctggttc aatcccgcca cctggccgaa ctgccgctcc
     2341 aacggcgcgg tcaccgccga agccatcaca tccgggcttg ccccgggata aaatgtgatc
     2401 acttgaatcg tcggaaaatc cacttccggc aacgccgaaa ccggcaactg ccggtacgcc
     2461 accactccca ccagcaatat cgccgccatc aacaacgatg tcgccaccgg acgcaggatg
     2521 aatggacgtg atgggttcac ttggagggag cgtttgtttt gccctttgaa ttcctcgggc
     2581 cggccggtgc ggcgtttgtg ctcggacttt cgccgggttt tagcgctttg actttcacgc
     2641 cttcaccgag cttgttgaaa ttatcaatgg cgatcaattg accggtctcc acaccttcaa
     2701 ccgccgtcga attggtgtcg cttacgccca ccttgatatt ttgcatggtg acgacctcat
     2761 tggtgaccat gttcgtgaag gtattatggt ttgtggtcac gacttcattc gcgactttgt
     2821 tcgtgaccac ataaacgaag gcgccttgcg gattgcgctg gatcgcaaat gttgggatca
     2881 agagttgatt ggtgagcacc tcgatcacca gccgcgcatt gacgaattga tttggaaaaa
     2941 gcgcgaagtc ttcgttgtta aaagtggcct tgatgcggat ggtcccggtc gcggtgtcaa
     3001 tcaggttgtc catcgtcagg tattcgcctg tggcaatttt ctttgactcc gtgcggtcat
     3061 aggcctcgac caacatggaa tgcccggctt gcacctggcg ctggatctgc ggcagaaaat
     3121 cttccgcgac actgaaaagc accgtgatcg gttgcaactg cgcgatggcc agcatggcat
     3181 tggtgttcgc ggcatgaacg acattgccgg gatcaaccag ccgaagcccg acacgcccgg
     3241 aaatcggcgc gcgaatgtag caataagcga gttggacccg ggcattggcc aactggccct
     3301 gatcaaactt caccgtccct tcatcttgat gcaccacccc ttgttggtca gccaattgct
     3361 gttccgggat ggctttgcgc tcgtatgcgg cctggaaacg tttgaggtcg atccgtgcga
     3421 tgtccaattg cgcctggtcg cgcgccaatt gcccttccgc ctcgatttcc aacgcctcga
     3481 acggtcccgg atcaatgacg gccagcaggt cgtttgtctt gaccaactgg ccttcggtgt
     3541 atgccacctt gatgagctgc ccgtcaacac ggggtgaaat ccccacagta taaacgggca
     3601 ggacggaacc aagcgccgtg acggagacgg caatgtcgcc gagttggatg ttggtggtgg
     3661 agatgggaat cggaggcggc ggcgcgggtt ttggcttgtg ccgccgcaga aagaaaatca
     3721 cgccgacaac caccaaaacc aaaaaaataa tcaaccaaat ccatccatga cgccttttac
//
LOCUS       DASZMM010000464         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3499509, whole genome shotgun sequence.
ACCESSION   DASZMM010000464 DASZMM010000000
VERSION     DASZMM010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499509"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VG754_11140"
     CDS             <1..235
                     /locus_tag="VG754_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWF19816.1"
                     /translation="GTFTNGKGRVQKFMKAVEPRGDARPEWEFLHELVYSVTGQNGFA
                     SIEGLFNQMAAEIPAFNGLTWAGLGETGATVPI"
     gene            243..1349
                     /gene="nuoH"
                     /locus_tag="VG754_11145"
     CDS             243..1349
                     /gene="nuoH"
                     /locus_tag="VG754_11145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWF19817.1"
                     /translation="MQHLPIFLSAISLPEPGSTGAFVLWSVVKIVVAIGVLLTMVAYS
                     VLVERKVSAFIQDRVGPNRVTLPLIGSIPVIGPFLAKCGIWQPLADGLKSFLKEDFTP
                     AHVRKVYFWLAPAITMIPAFLTVAVIPFGSTLGHQQMVIANLNVGILYTFGIVSLGVY
                     GIVLAGYAANSKYPFLGGIRSSAQMISYEIAMGMSVIPVFMLVGSLNLSDVIAYQTHC
                     WLVFKQPIAFIVFLVAAFAETNRLPFDMPECETELVAGYNTEYSSMKFAMFFMGEYAN
                     MIASSAMMVTLFLGGWTLPGFDQPTATIAGGLLSIFIFLAKVCLLMLLFIWVRWMMPR
                     FRYDQLMHLGWQRFIPLTLANIFVTALVLWLLAR"
     gene            1357..1596
                     /locus_tag="VG754_11150"
     CDS             1357..1596
                     /locus_tag="VG754_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HWF19818.1"
                     /translation="MPTLLRIRGFRVFFFSNEGTEPPHVHVRKGDGVAKFWLGPIKLA
                     VNDGFKKSELQIIIKILEEHEAELIRKWNEIEQAT"
     gene            1610..1873
                     /locus_tag="VG754_11155"
     CDS             1610..1873
                     /locus_tag="VG754_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWF19819.1"
                     /translation="MTTADRLTVSYDDGRIVSLPLKWYPRLNRATPAQRRNWELIGRG
                     YGVHWPDVDEDLSAEGLLEGRPAREFIRAGNRRGTRHNQPVSA"
     gene            1870..2403
                     /locus_tag="VG754_11160"
     CDS             1870..2403
                     /locus_tag="VG754_11160"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWF19820.1"
                     /translation="MIVKRDKLTFWERLYLPAIIGGFKVTLRHFFGKKVTMQYPEEKW
                     VVPPGYRGAPYLVKDQEGRTKCVSCQLCEFVCPPKAIRIMPPGPQGSPEVGNVEKAPK
                     EFDINMLRCIFCGFCQEVCPEEAIFLMKDYSLTGTNRQEMIFNKEKLLKLGGIHEDKI
                     QKWKHKAEEAKAQGLAP"
     gene            2426..2914
                     /locus_tag="VG754_11165"
     CDS             2426..2914
                     /locus_tag="VG754_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWF19821.1"
                     /translation="MQDILFYIFAALALICGALVVLSRNPVNSAMFLVLTIVSLAALF
                     VLLHAFFLAAVQVVVYAGAVIVLFLFVIMLLDLKEEHRRKIKTFGATTGIISVMAIGL
                     VFWRSLAHTSLPAGSEPAEGGTLALGRLLFTNYALPFEILSVLLLVAMIGVILLSKKD
                     LK"
     gene            2929..3576
                     /locus_tag="VG754_11170"
     CDS             2929..3576
                     /locus_tag="VG754_11170"
                     /inference="COORDINATES: protein motif:HMM:NF025919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HWF19822.1"
                     /translation="MSLYNQIETRYGFKIPEEYRRLEEKGWLNWNDKSTDYLWIDDGE
                     WLTPEQILDYKFKSYHKPGFVPFARTGSGDAWCWYPEWSVNGIAPVVFCPHDCYDGNC
                     YAPSFLGWIFRTIVEFASSGRCADEASAREYFAQWQKMVVPMMPPAWAAIISGFEKKQ
                     FRTWPEEQGFGGSGFISMKEADEIVKQHLEFPHLNEELQWMTPVRKWNLSHLDKK"
     gene            3573..3878
                     /gene="nuoK"
                     /locus_tag="VG754_11175"
     CDS             3573..3878
                     /gene="nuoK"
                     /locus_tag="VG754_11175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWF19823.1"
                     /translation="MNVGLQHYMVVSGLLFSLGLLGVIMRRNLLVIYMCLELMLNSAN
                     LALVALSRFNGNLDGQIMVFFIITVAAAEVAVGLALIVALYRKRQTAHVEDLTSMKY"
     gene            3882..>4158
                     /locus_tag="VG754_11180"
     CDS             3882..>4158
                     /locus_tag="VG754_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWF19824.1"
                     /translation="MFDNPEKLAWVILFLPLAATVIITVFTRNHKRLSGQISIGAVLV
                     SFLITVVLFAMLRSSGQDKLPQVHSTWLAIDDFVAEIGLRLDYLSLTM"
BASE COUNT          887 a         1023 c         1222 g         1026 t
ORIGIN      
        1 cggcacgttc accaacggca agggccgcgt ccaaaagttc atgaaagccg tcgagccgcg
       61 cggcgatgcg cgtcccgagt gggaattttt gcacgaactg gtttacagcg tgaccggcca
      121 gaacggattt gccagcatcg aaggcttgtt caaccagatg gcggcggaaa tcccggcgtt
      181 caacggtttg acgtgggccg ggctgggtga gacgggcgca acggtgccga tttgatttag
      241 caatgcaaca tctgccaata tttttgagtg ccatatcttt gccggaaccc ggttcaacgg
      301 gcgcattcgt cctttggagc gtggtgaaga ttgtcgtcgc gatcggcgtt ttgctgacga
      361 tggtcgcgta ttctgtcttg gtcgaacgca aggtttcggc gttcattcaa gaccgcgttg
      421 gaccgaaccg cgtcacgctg ccgttgattg gcagcattcc ggtcatcggg ccgttcctcg
      481 ccaagtgcgg catctggcaa ccactggcgg acggcctcaa atcttttctc aaggaagatt
      541 tcaccccggc gcatgtgcgc aaagtttatt tctggctcgc gccggccatc acgatgattc
      601 ccgcgttcct caccgtcgcg gtgattccct tcggctcgac gctcggtcat caacaaatgg
      661 tgatcgccaa cttgaacgtg ggcattcttt acacgtttgg catcgtgtcg ctcggcgtgt
      721 atgggattgt gctggcgggt tacgcggcga actcgaaata tcctttcctc ggcggcatcc
      781 gttccagcgc gcaaatgatt tcgtatgaaa tcgcgatggg aatgtcggtg ataccggtgt
      841 tcatgctcgt gggaagtttg aatctcagcg atgtcattgc atatcaaacg cactgctggc
      901 tcgtgttcaa gcaaccgatt gccttcatcg tttttttggt ggctgccttt gcggaaacga
      961 accggctgcc gttcgacatg cccgaatgcg aaactgaact ggtcgccggt tacaacaccg
     1021 aatacagctc gatgaagttc gcgatgtttt tcatgggcga atacgcgaac atgatcgcgt
     1081 catcggccat gatggtgacg ctctttttgg gcggctggac gctcccgggg ttcgatcaac
     1141 caaccgcaac gattgcaggc ggcttgttga gcatcttcat tttcctggcg aaagtctgcc
     1201 tgttgatgtt gctcttcatc tgggtgcgat ggatgatgcc gcgctttcgg tatgatcaat
     1261 tgatgcatct gggctggcag cggttcattc cgctgacgct cgcgaatatt ttcgtgacgg
     1321 cgttggtgct gtggttgctg gcaagatgac aacaccatgc ccacactttt gcggattcga
     1381 gggtttagag tgttcttctt cagcaatgaa ggcactgaac ctccacatgt gcatgtgcgc
     1441 aagggcgatg gcgtggcaaa attttggctt ggcccaataa agctggcggt taacgacggc
     1501 tttaaaaaga gcgaattgca aattatcata aagatattgg aagaacacga agcggaatta
     1561 attcggaaat ggaatgaaat cgaacaagca acctaaaatt caggatgtca tgacgactgc
     1621 ggaccggttg acggtcagtt atgatgacgg acggattgtc tcgctgccgt tgaaatggta
     1681 tccgcgattg aaccgggcaa cccccgcgca acgccgcaat tgggaattga tcggccgcgg
     1741 ttacggtgtg cattggcccg atgtggatga agatttgagc gccgaaggac tgctggaagg
     1801 ccgtcccgcc cgggaattta ttcgtgccgg caatcgccgt ggaacccggc ataaccaacc
     1861 cgtttcggca tgatcgtcaa gcgcgacaaa ctgacttttt gggagcggct gtatctgccg
     1921 gccatcatcg gcgggttcaa agtcacgctc cgccatttct tcggcaagaa agtgacgatg
     1981 caatatcccg aggaaaaatg ggtcgtccct cccggttatc gcggcgcgcc gtatctggtg
     2041 aaagaccagg agggccgcac gaaatgcgtt tcgtgccagt tgtgcgaatt cgtctgcccg
     2101 cccaaggcca tccgcatcat gccgcccggc ccgcaagggt cgccggaagt cggcaacgtc
     2161 gagaaagcgc ccaaggaatt tgatatcaac atgctacggt gcatcttctg cggcttctgc
     2221 caggaagttt gcccggagga agccattttt ctgatgaaag attattcgct gaccggcacg
     2281 aaccggcagg agatgatttt taacaaggaa aaactgctca agctgggcgg catccatgag
     2341 gacaagattc agaagtggaa acacaaggcc gaagaagcga aggcgcaagg tttggcgccc
     2401 tgaccggcag gaaccctgaa gatcaatgca ggacattttg ttttacatat tcgcggcgct
     2461 ggcgctgatt tgcggcgcgc tggtcgttct gagccgcaat ccggtcaaca gcgcgatgtt
     2521 cctggtgctg acgatcgttt cgctggccgc gttgtttgtg ttgttgcacg ctttttttct
     2581 cgcggcggtg caggtggtgg tttatgcggg ggcggtcatt gtgcttttcc tgttcgtcat
     2641 catgctgctg gatttgaagg aggaacaccg gcgcaagata aaaactttcg gcgcgacgac
     2701 gggaatcata tcggtgatgg ccatcggttt ggttttctgg cggtcattgg cgcatacaag
     2761 tctgccggca ggatcggaac cggctgaagg aggcacattg gcactcgggc ggttgctgtt
     2821 cacgaattac gcgctgccgt ttgaaatttt gtctgtgctg ctgctggtcg cgatgattgg
     2881 cgtgattttg ctgagcaaga aagaccttaa gtaaacagat tcaccgccat gagcctctat
     2941 aaccaaatcg aaactcgcta cgggttcaaa atcccggagg agtatcgtcg tttggaagag
     3001 aaaggctggc tcaactggaa tgataagagc actgattacc tctggattga tgacggtgaa
     3061 tggctcacgc cggagcagat tctggattac aaattcaagt catatcataa accgggcttt
     3121 gtcccgtttg cccgcactgg aagcggtgat gcatggtgtt ggtatccgga gtggagcgtg
     3181 aatggcattg ctcccgtcgt gttctgcccg catgactgtt acgacgggaa ctgttatgcc
     3241 ccaagtttcc ttggctggat atttcggacg atcgtcgagt ttgcctcttc tggcagatgc
     3301 gctgatgaag cctccgcgcg ggaatatttt gcgcaatggc aaaaaatggt cgtgccgatg
     3361 atgccgcctg cctgggcggc gataatcagc ggctttgaaa aaaagcagtt taggacctgg
     3421 ccggaagagc agggatttgg cgggtcgggg ttcatttcga tgaaggaagc ggatgaaatt
     3481 gtaaaacagc atctggagtt tcctcatctc aacgaagaac tccaatggat gacccctgtg
     3541 aggaaatgga atttgagcca cttggataaa aaatgaacgt cggccttcaa cattatatgg
     3601 tcgttagcgg gctgcttttc agcctcgggt tgctgggcgt catcatgcgc cgcaatctgc
     3661 tggtgattta catgtgcctc gaattgatgc tgaactccgc gaacctcgcg ctcgtcgcgt
     3721 tgtcccgttt caacggaaat ttggacgggc aaatcatggt gttcttcatc atcaccgttg
     3781 ccgcggcgga agtcgccgtg ggactggcat tgatcgtcgc gctttatcgc aagcgccaga
     3841 cggcgcatgt ggaagacctg acttcgatga aatattagaa catgttcgac aacccggaaa
     3901 aattggcgtg ggtgattttg tttctgccgc tggcagcgac cgtcatcatc acggttttca
     3961 cgcgcaacca taaacggttg agcggacaga tttccatcgg cgcggtgctg gtttcgttcc
     4021 tgattaccgt ggtgctgttt gcgatgttac gttcgtcggg gcaagacaag ctgccgcagg
     4081 tgcattcgac ctggcttgcc attgatgact tcgttgcgga gatcggattg cgcttggatt
     4141 acttgagcct cacgatgg
//
LOCUS       DASZMM010000465         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1601651, whole genome shotgun sequence.
ACCESSION   DASZMM010000465 DASZMM010000000
VERSION     DASZMM010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601651"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VG754_11185"
     CDS             complement(<1..800)
                     /locus_tag="VG754_11185"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWF19825.1"
                     /translation="MKKRLILPAVAALCLLAIPYADAQFTTSNFFNWETTPVHPVALS
                     PDGTRLAVCNLPDDHVEIFNVTSGVPVSLASIAVGLNPVSVCFRTTNELWVANYISST
                     LSVIDLATMRVFNTINTTNQPSDIVFAGASNLAYVSCGQPNVVQVFNPISSQLVTNLN
                     IDGNRPRAMSVSPDGSKVYVAIFESGNASTIISTGVSDLNNLPKPSPITFPDAPSHGL
                     DPPPNSGTNFVPAINPALTNPPPRTGLIVKKNSAGRWMDDNNGDWTEF"
     gene            complement(1006..>1542)
                     /locus_tag="VG754_11190"
     CDS             complement(1006..>1542)
                     /locus_tag="VG754_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF19826.1"
                     /translation="LYEGRNIANLPPHQIVKLGLSHVPEGRMVFANLTVMENLMMGAY
                     LQKDQKVIKQELDYIFGIFPRLQERQKQVAGTLSGGEQQMLAIGRALMSKPKFLMLDE
                     PSLGIAPLLVKTIFEKIVEINRQQGISILLVEQNANLALEISRYGYVLETGRIILQDA
                     SAALRQSPQVKSAYLGGG"
BASE COUNT          338 a          409 c          441 g          354 t
ORIGIN      
        1 atgaactccg tccagtcgcc attgttgtcg tccatccagc ggcccgcgct gttctttttc
       61 acgatgaggc cggtgcgcgg tggcggattg gtgagcgcgg gattgatggc gggaacgaaa
      121 ttcgtgccgc tgttcggtgg cgggtccaac ccatgcgacg gcgcgtcggg aaaggtgatg
      181 ggacttggct tcggcaaatt gttcaaatcc gaaacgccgg tgctgataat cgtcgaggcg
      241 ttgccggatt cgaaaatcgc cacatacacc ttgctgccgt cagggctgac gctcatcgcg
      301 cggggacggt ttccatcaat gttaagattg gtgacgagtt gcgaagaaat cggattgaac
      361 acttgcacga cgtttggctg gccgcaggaa acgtaagcaa ggttcgacgc gcccgcgaaa
      421 acgatgtccg aaggctggtt ggtcgtgttg atggtgttga agacgcgcat cgtggcgagg
      481 tcaatcacgc tgagcgtgct ggaaatgtaa ttggcgaccc aaagttcgtt ggtcgtgcgg
      541 aaacagacac tcaccgggtt caagcccacg gcaatgctgg ccagcgaaac cggcacgcca
      601 gaggtcacgt taaatatttc gacatggtcg tcaggaagat tgcaaacagc gagcctcgtg
      661 ccgtcgggac tgagcgcaac cggatgcacc ggtgtcgttt cccaattgaa gaaattgctt
      721 gtggtgaact gcgcgtccgc gtacggaatg gccagcaggc aaagagctgc gacggccggc
      781 aatataagcc tttttttcac gcaagctttt gaccttgtcc acgcaacggg gcaatgacac
      841 ttctgattca tgcgggcagt gtcggtgtta tcgggtttcc tgttgtgctg tcgttttaca
      901 gcaaactgac gaatttgaat agtgtgcgtt tgaatgcaca cccggcgaat gcattacgaa
      961 actttccgcg gaggcgagcg tagtcgcgag ccgtaatccg atttctcaac cgccgcccag
     1021 atacgcgctc ttcacctgcg ggctttgacg aagcgctgcg gaagcgtcct gcaaaatgat
     1081 gcggccagtt tcaagcacgt acccgtagcg cgaaatttcc agcgcaagat tcgcgttctg
     1141 ttcgaccagc aagattgaaa tgccctgttg ccggttgatc tcgacaattt tctcgaaaat
     1201 cgtttttacc aacagcggcg cgatgccgag cgaaggctcg tcgagcatca aaaattttgg
     1261 cttggacatc aacgcgcggc cgatggccag catctgctgt tcgccgcccg acagcgtgcc
     1321 cgccacctgt ttctgccgct cctgcaatcg cggaaaaatg ccgaagatat aatccaattc
     1381 ctgcttgatg actttttggt ccttttgcaa atacgcgccc atcatcaagt tttccatcac
     1441 cgttaaattt gcgaacacca tccggccctc cggcacatgc gacaggccga gcttgacgat
     1501 ttgatgcggc ggcaaatttg cgatgttacg cccttcataa ag
//
LOCUS       DASZMM010000466         7616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5428057, whole genome shotgun sequence.
ACCESSION   DASZMM010000466 DASZMM010000000
VERSION     DASZMM010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5428057"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VG754_11195"
     CDS             complement(<1..221)
                     /locus_tag="VG754_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19827.1"
                     /translation="MSNVFASMIFGSPAVGERTREPSWQAHPRTTPDLLAMPGGNGRT
                     IETERGSMSRSNARRTGHVRLIHAHLSFP"
     regulatory      254..336
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            371..769
                     /gene="crcB"
                     /locus_tag="VG754_11200"
     CDS             371..769
                     /gene="crcB"
                     /locus_tag="VG754_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWF19828.1"
                     /translation="MQTYLLVALGGALGSVARFWLGGMIGARMGETFPWGTVIVNITG
                     SFLIGLFFAMTEPGSRWLAPPGARAFLMTGICGGYTTFSSFSLQTLNLLRDGQWLYAG
                     GNVIFSMVLCLFAVWLGFLLGTVFNSMKGS"
     gene            771..1115
                     /locus_tag="VG754_11205"
     CDS             771..1115
                     /locus_tag="VG754_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HWF19829.1"
                     /translation="MQIPQQAMLLRILIGESDRWQHQPLYEAIVLKAREAHLAGATVL
                     RGPMGFGKSSRLHTAKILRLSMDLPLVIEIVDSEEKIRAFLPALDSMIGGGLVTLEKV
                     EVVHYRSAETKE"
     gene            1287..3770
                     /locus_tag="VG754_11210"
     CDS             1287..3770
                     /locus_tag="VG754_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HWF19830.1"
                     /translation="MSAFAQEPNNNPFSVESVPGWVNPIAAPDKTPSTTHSSREGEHY
                     LLLDQQENAVIDETYSHVVKEITSENGVQNGSKVQFQYEPAYQKLSVHFVRIIRDGKI
                     LDRLEPEKFKVIQQEPGLERHLFMGELSAVLFLEDVRIGDIIEYACTTTGSNPILGGH
                     FVSSFLAQWSTPMDRQSFRLLWPSGKELHIKNHKTTAQPEIRKLGELTEYDWNLFDIP
                     TSVYEDGTPSWVESFGWVQLSDFSNWGEVAQWAMPLYQHKRGELPAELRAKIAVWQYH
                     ATSDEQVARALRFVQDDVRYLGIEIGANSQQPNRPELVFNRRFGDCKDKVSLFCSILD
                     EMGIQARPVFVHSYFRKTVEDWCPSPYDFNHVVAQVKLNGKVLWLDPTLSYQRGPLTN
                     IFFPDYGCGLVIAPETTELTQIPTQTAGEPLTSIYDTFRVFSYTNPAAYEVRITRRGV
                     DADELRAVLAETSREALEKSQLNYFARLYPKIKTSAPLEVRDDEKKNEITLYLNYRIS
                     DIWELSENKRKWTLDLHAGEIFNMAREPQTSLRTLPILVLHPMHRKQVTSFNFIGPAS
                     FKNETIKFEDQAVRFERQAAFSQGKLTISYDCATRTNMVPVAEASEYLATLKRIREKC
                     YYEVERPVFDPTHVQYQPNWPVLALASVFSVLMLPAMFLAYHFRPNSAQPPQLQSDRA
                     LTGLAGWLILVAISLGFRIVFTIIQIFKLLWACSLEKWQLLTIPGSTAYNPNWGPILI
                     FELFANLAFLAFAILLLILYFQKRFALPRYIIGFLIATVVFILIDNAWLVAIKPATVI
                     KSIVPMVLGVGIWVWYFLVSRRVKLTFIC"
     gene            3826..5154
                     /locus_tag="VG754_11215"
     CDS             3826..5154
                     /locus_tag="VG754_11215"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWF19831.1"
                     /translation="MKHVTLGMIGGGTVGSGVFHAWQTNGDLMAARLGVKLDFRRIAV
                     KAFDEPRPYPIPDALMTTDWQQVVNDPQIQIVIELVGGMGIAKTMILTALAQGKTVIT
                     ANKALLSAHGRELFAAAARHGANLYYEASVCGGIPIIKSLREGFVANQFPAIYGIVNG
                     TCNYILSRMKQEGAEFGDVLADAQKLGYAETPPDLDIDGHDAQHKIGILASLAHGFWV
                     DHHEIHTEGIRNISKLDMQYAQQLGYTIKLLGIAKQQAISKSKTKKQKTQIQVSVYPA
                     LVPNAHVLAGVNGVFNAVFVRGDIVGDTLFYGRGAGKDATASAVLSDVADAALDLKCG
                     TKSRVCPFVPHQRDGAVLPIGEVISRYYVRLTVIDKPGTLAKITAILGAAKIGISSII
                     QPEGHEGSTVPLVLMTHSATNADMTQALKKIGKLNAVKGEPVMLRVENFD"
     gene            5561..6913
                     /gene="thrC"
                     /locus_tag="VG754_11220"
     CDS             5561..6913
                     /gene="thrC"
                     /locus_tag="VG754_11220"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWF19832.1"
                     /translation="MIQFRSTNEKSPAVNLREAFLRGIAPDGGLYFPEKFPTLSCEEI
                     AGFAKLPYYEIAFRVVSKYTGGLVPDADLAALCREAYNFDVPLELVYDRVHVMRLDCG
                     PTASFKDFAARMMARLMGRFLREEGRQLTILTATSGDTGSAVAHAFHNVPGIRVIVLF
                     PFAEVSARQRKLMTTLRDNVRTVAIDGKFDDCQAMVKRAFADPALKHIELSSANSINI
                     GRLLPQSVYYFYAASRLAKPGEPIVFSVPSGNFGDMMGSAVAKQMGLPVKKIIASVNE
                     NDAFPRFLATGVYQKVSPSRNSLSNAMNVGHPSNLARLVAIYGGQMDEIGKIHRAPDL
                     AAMRRDIYSSSISDERTRETLKEAWTKHKLLLEPHGAVAWRGFLDWQKTEPLEGLPAA
                     ILETAHPAKFPEEIEKTLGFTLYVPPTIAVSERLPEDYDRMGPDYEKFKEYLLAKHAK
                     "
     gene            6961..7239
                     /locus_tag="VG754_11225"
     CDS             6961..7239
                     /locus_tag="VG754_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19833.1"
                     /translation="MKTLTITKARANLSALLEKAKRGEDIGIISGDQIVALRPVTVSS
                     DDYAWREYGATPAELQDFEKRMEQTITKETKRGKIKRFSGNLEKDLTD"
     gene            7236..7514
                     /locus_tag="VG754_11230"
     CDS             7236..7514
                     /locus_tag="VG754_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19834.1"
                     /translation="MKSLLLSEMFRANTRAYPKETRRKIGQALQGLETDFGHPHRHQG
                     LGIRKLTGNFFEIRVGLDVRLVFQNAPDSLLFLMAGNHDEAQKFLRGR"
BASE COUNT         1705 a         2064 c         2099 g         1748 t
ORIGIN      
        1 tgtgggaagg acaggtgcgc atgaattaat ctgacatgtc ctgttcgtcg cgcgttgctg
       61 cggctcatcg agccgcgctc cgtttcgatg gtccggccgt tccctcccgg catggccaac
      121 aaatcggggg tagtgcgcgg atgcgcctgc caagatggct cgcgagtacg ctcgcccacc
      181 gccggagaac caaaaatcat cgaagcgaat acgttcgaca tttgaattgc caagcagaga
      241 cggcaatctt attaattggg atggcaatgg ggtttgccgt cccgaaatgg gaaaaccgcc
      301 tgactcgttt tcagcgctga tgactcctga ctgcggcatt gcatggacat gtcgtcgccg
      361 ggagtttttt atgcaaacat atcttttggt ggccctgggt ggagcgcttg gcagcgtggc
      421 ccggttctgg ctgggcggga tgatcggcgc aaggatgggc gaaacatttc cctggggcac
      481 cgtgatcgtc aacatcaccg gctctttttt gatcggcctg ttttttgcca tgaccgagcc
      541 cggctcgcgc tggctggcgc ctccaggggc gcgggcattt ttgatgacgg gcatttgcgg
      601 cggttacacg acgttttcat cgttcagttt gcaaacgttg aatctgctcc gcgatgggca
      661 atggttgtat gcgggcggca acgtgatatt ctcgatggtg ctttgcctgt ttgctgtgtg
      721 gctcggcttc cttctcggca cggttttcaa ttccatgaaa ggctcctgat atgcaaatcc
      781 cccaacaggc aatgctcctc cggattttga tcggcgaaag cgatcgctgg cagcatcaac
      841 cgctctatga agccatcgtg ctcaaggcgc gcgaagcgca tctggcgggc gcgacggtgt
      901 tgcgcggacc gatgggtttc ggcaaatcaa gccgcctgca cacggctaaa attttgcggc
      961 tctcaatgga cttgccgctg gtcatcgaga ttgtggacag cgaggaaaaa atccgcgcgt
     1021 tcctgcccgc gctcgacagc atgattggcg gcggcttggt gacgttggaa aaagtggagg
     1081 tggtgcatta ccgctcggcg gagacgaagg agtgataggc gatttttatt ttaatttttg
     1141 cgtgcttggg gtaattgcaa gcagtgcgat tgctcaatca tgaagggggt ttcggccact
     1201 tgcgggttaa aagtgcggag attgcaggcc atcatttaaa attttggctg tatggaatct
     1261 tatgccttgc tgccgggctg tgtgccttgt ccgcttttgc acaagagcca aataacaacc
     1321 ctttcagcgt cgaatccgtg cccggctggg taaatcccat cgccgcgcca gacaagacgc
     1381 cttccacaac acattcttcg cgcgaaggcg agcattatct gctgctcgac cagcaggaga
     1441 atgcggtcat tgatgaaacc tattctcatg tggtgaagga gatcaccagt gaaaacggtg
     1501 tgcagaacgg ctcgaaggtg caatttcaat atgagccggc ctaccagaaa ttgagcgttc
     1561 attttgtgcg catcatccgg gatggaaaaa tcctcgaccg cctggaaccg gaaaaattca
     1621 aggtgatcca gcaggaaccg ggactcgaac gtcatctgtt catgggcgaa ttaagcgcgg
     1681 tcctgttttt ggaggatgtg cggatcggcg acatcatcga atacgcctgc acgaccacgg
     1741 gttcgaatcc cattttgggc gggcattttg tgagttcctt cctggcgcag tggagcacgc
     1801 cgatggaccg gcaaagtttc aggttgttat ggccgtctgg aaaagagctg cacatcaaaa
     1861 atcataaaac caccgcgcag ccggaaatcc gcaagctggg tgaactgacc gaatatgatt
     1921 ggaacctctt cgatattccg acctcggtct atgaggatgg gacgccgagt tgggtggaaa
     1981 gcttcggttg ggtgcagttg agcgattttt cgaactgggg cgaagttgcg caatgggcaa
     2041 tgccgcttta ccagcacaag cgtggcgaac tgcccgcaga actgcgcgcc aaaatagcag
     2101 tctggcaata tcacgcaact tccgacgaac aggtggcgcg ggcgttgcgt ttcgtgcagg
     2161 acgacgtgcg ttacttgggc atcgaaattg gtgccaattc acaacagccc aatcgtccgg
     2221 aactggtttt caaccgtcgt tttggcgatt gcaaggacaa ggtgagtctt ttctgctcca
     2281 ttttggacga gatgggcatt caagcgcgcc cggtgtttgt gcattcttat tttcgcaaaa
     2341 ctgtcgagga ttggtgtcct tcaccgtacg acttcaatca tgtcgttgcg caggtgaagc
     2401 tcaatgggaa ggtgctgtgg ctcgatccca cgttgtccta tcaacgcggc ccgttgacca
     2461 atattttttt ccccgattac ggctgcggcc tggtcatcgc ccctgaaacc acggaactca
     2521 cgcaaattcc cacacagacc gccggtgaac cgcttacgtc catctatgac acgtttcgcg
     2581 tgttttccta caccaatcct gcggcatacg aggtccgcat caccagacga ggcgtggatg
     2641 ccgatgagtt gcgggccgtc cttgccgaga cgagccggga agcgctggaa aaaagccagt
     2701 tgaattattt cgcacggttg tatcccaaaa tcaaaacgtc ggcgcctctc gaagtccgcg
     2761 acgatgagaa aaaaaacgag atcaccttgt atctaaatta tcggatttcg gacatatggg
     2821 agctttccga gaacaagcgc aaatggacac tcgatctgca cgccggggaa atctttaaca
     2881 tggcgcgcga accgcaaacg tctctgcgca cgcttccgat tttagtcttg catccaatgc
     2941 atcgaaaaca agtcacgagc ttcaacttta tcgggccggc ctcgttcaag aacgaaacca
     3001 tcaaattcga ggatcaagcc gtgcgtttcg agaggcaggc ggcattttcg caggggaaat
     3061 tgaccatcag ttatgattgc gccacacgca cgaacatggt cccggttgcc gaagcgtccg
     3121 aatatcttgc cacgctcaag cgcatccggg aaaagtgcta ttacgaagtc gagcgcccgg
     3181 tcttcgatcc aacgcacgtc caatatcaac ccaattggcc ggttctcgcg ctcgcgtcag
     3241 ttttctccgt gctgatgctt ccggcaatgt ttctcgcgta ccattttcgg cccaacagtg
     3301 cgcaaccgcc gcaactccag tccgatcggg cgctgaccgg ccttgcgggc tggctgatac
     3361 ttgtcgccat cagcctcggc tttagaattg tcttcaccat catacaaatc ttcaagctcc
     3421 tgtgggcttg ctcgctggaa aagtggcagt tgctgaccat ccccggctcg accgcttata
     3481 accccaactg ggggccaata ttgatttttg agctttttgc caatctcgct ttccttgctt
     3541 ttgccatcct ccttttgatt ctttacttcc agaagcggtt tgccctgccc cgatatatca
     3601 tcggtttcct gatcgcaacc gtagttttca tcctgattga caatgcttgg ctggttgcaa
     3661 taaagccggc caccgtcatc aagtccattg tgccgatggt gttgggtgtg gggatttggg
     3721 tctggtattt tctcgtgtcc cgaagggtca aactcacttt tatttgctga actgccttaa
     3781 aaagaaattg tcgcggcgga tttttcgcgg catcctgaag gctttatgaa gcacgtgact
     3841 ctcgggatga ttggcggcgg caccgtcggc agcggcgttt ttcacgcctg gcagacgaac
     3901 ggcgatttga tggccgcgcg ccttggcgtg aagctcgatt tccgccgcat cgcggtcaag
     3961 gcgttcgatg aaccgcgccc ctaccccatt cccgatgcat tgatgacgac cgactggcaa
     4021 caggtggtca acgatccgca aatccagatc gtgatcgaat tggtcggcgg catgggcatc
     4081 gccaagacga tgatcctcac cgcgcttgca cagggcaaga cggtcatcac cgcgaacaag
     4141 gcattgcttt cggcgcatgg ccgcgaactt ttcgcggcag cagcgcgaca cggcgccaat
     4201 ctttattacg aggcgagtgt ctgcggcggc ataccgatca tcaaatcttt gcgcgaaggt
     4261 ttcgtggcga accaatttcc cgccatctat ggcatcgtca atggcacctg caattatatc
     4321 ctttcgcgca tgaagcagga aggcgcggag ttcggcgacg tcctcgccga tgcgcaaaag
     4381 ctgggttatg ccgagactcc gccggacctc gacattgacg ggcacgatgc ccagcacaag
     4441 atcggcattc ttgcttcgct ggcgcatggt ttttgggtgg accatcatga aatccacacc
     4501 gagggcattc gcaatatttc caagctggac atgcaatacg cgcagcaact cggctacacc
     4561 atcaaattgc tgggaatcgc caaacaacaa gcaatttcca aatcgaaaac aaaaaaacaa
     4621 aaaacgcaaa tccaagtctc cgtctatccg gcgctggtgc cgaacgctca cgtcctcgcc
     4681 ggcgttaacg gcgttttcaa tgcggttttt gtgcggggcg acatcgttgg cgatacgctt
     4741 ttttatggtc gtggcgcagg caaggacgcg accgccagcg cagttttgag cgatgttgcg
     4801 gacgccgcgc ttgatttgaa atgcggcacc aagagccgcg tctgcccttt cgttccgcac
     4861 cagcgcgacg gggcagtcct gcccatcggc gaagtcatct cgcgctatta cgtgcgcctc
     4921 acggtgattg acaaacccgg cacgctggca aaaatcaccg ccattctcgg cgcggccaag
     4981 attgggattt cttccatcat ccaacccgaa ggtcacgaag gcagcacagt gccgctggtg
     5041 ttgatgaccc acagcgcgac caacgccgac atgacgcaag ccttgaaaaa aatcggcaag
     5101 ctcaacgcag tcaaaggcga acccgtcatg ctgcgcgtgg agaattttga ttgatgagcg
     5161 ccgtccactc acctcaagaa aaaaaacggc tgagttatgc tcgcgatcat tatgcgaaag
     5221 gaaagcatga taagggcttt cgtcgagcat ggcctcgtaa gcgggtaaag gccacaagag
     5281 catttcggca cgcacttgag gttttgactc gcctggcaag agaaaatccg gatgctgatt
     5341 tccaaacacc caaacggaag cgattaagga aatggggatc aagccatttg aaggaaattg
     5401 tgggacggaa aagggataaa agagttcgtt cagaagggtc aaaaaagatt agaaagttga
     5461 ggcggcagaa ccatgcagtg atagtcgcat cactccgcac tccgcactcc gcactccgca
     5521 attccccgca tccttcgcgg tcttcgcgcg acacacactc atgatccagt ttcgcagcac
     5581 caacgaaaaa tcgcccgcgg tgaacctgcg cgaagcgttc ctgcgcggca tcgcgccgga
     5641 tggcgggctt tattttccgg agaaatttcc gacgctttca tgcgaggaaa tcgctggatt
     5701 cgcaaagctg ccctactacg aaatcgcgtt tcgtgtcgtg tccaaataca cgggcggcct
     5761 cgttcccgat gcagacctcg ccgccctttg ccgcgaagcc tacaactttg atgtgccgct
     5821 cgaattggtt tatgaccgcg tccatgtcat gcgtctggat tgcgggccga cggcttcgtt
     5881 caaggatttc gccgcgcgga tgatggcgcg tttgatgggc cgttttttgc gggaggaagg
     5941 ccggcaactg accatcctca ccgcgaccag cggcgacacc ggctcggcag tggcgcacgc
     6001 atttcataat gtgcccggca tccgcgtgat tgtgctgttc ccgttcgcgg aagtcagcgc
     6061 gcgccagcgc aagctcatga ccacgttgcg cgacaacgtc cgcaccgtgg cgattgacgg
     6121 caaattcgac gattgccagg cgatggtgaa gcgcgccttc gccgatcccg cgctgaaaca
     6181 catcgaactt tcctccgcca actccatcaa catcggccgg ttgctgccgc aaagtgtcta
     6241 ttatttttat gcggcgtcgc ggctggccaa accgggcgag ccgattgtct tttcggttcc
     6301 gagcggcaat ttcggcgaca tgatgggctc cgccgtggcg aagcagatgg gtttgccggt
     6361 caaaaaaatc atcgcttcgg tcaacgagaa cgacgccttc ccgcgttttc tcgcgactgg
     6421 tgtttatcaa aaagtttcgc cgtcgcgcaa ttcgctttct aatgcgatga atgtgggcca
     6481 ccccagcaat ctcgcgcggc ttgtggcgat ttacggcggg cagatggatg agatcgggaa
     6541 aattcatcgc gcgcccgact tggctgcgat gcgtcgcgac atttattcga gttcaatttc
     6601 cgacgaacgc acccgcgaaa cgctcaagga agcctggacg aagcacaagt tgctgctgga
     6661 accgcacggc gcggtggcgt ggcgtggctt tcttgattgg cagaaaaccg aaccgctcga
     6721 aggattgccc gcggccattc tcgaaaccgc gcatccggcc aagttccccg aggaaatcga
     6781 aaagacgctc ggtttcacgc tttacgtccc gcccaccatc gccgtctccg agcgcctgcc
     6841 ggaagactac gaccgcatgg gaccggatta cgagaaattc aaggagtatc tgctcgcgaa
     6901 gcatgccaaa taatcggagt tttgattgac aatacgcgtc tgagactcta aatagtcttt
     6961 atgaagacgc taaccataac gaaggcgcga gcgaaccttt cagcccttct ggagaaagcc
     7021 aagcgtggtg aagacattgg catcatttcc ggcgatcaaa ttgtggcact gcggcccgtg
     7081 accgtttcat cagatgatta tgcatggcgc gaatatggcg cgacgccggc ggaacttcag
     7141 gattttgaaa aacgcatgga acaaacaatc acaaaggaaa cgaagcgggg aaaaatcaaa
     7201 cgcttttccg gcaatctgga aaaggatttg accgattgaa gtccttgctg ttgagcgaga
     7261 tgtttcgggc caacacccgt gcgtatccca aggaaactcg tcgaaaaatc gggcaggctt
     7321 tacaaggctt ggaaaccgat tttgggcatc cacatcgcca tcaaggactt ggcatccgaa
     7381 agttaacggg gaattttttc gaaatccgcg tgggacttga cgtgcggctg gtttttcaaa
     7441 atgcccctga ttcgttattg tttttgatgg cgggcaatca cgatgaagcg cagaaatttc
     7501 ttcgcggacg ttagggccaa attccacctt ttcaaacccc gatattctgg taacttctcc
     7561 atcccgtttt ggctttgacc gcggctgttt cgcacatgac caaacggttt tatggc
//
LOCUS       DASZMM010000467         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2676310, whole genome shotgun sequence.
ACCESSION   DASZMM010000467 DASZMM010000000
VERSION     DASZMM010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676310"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2885)
                     /locus_tag="VG754_11235"
     CDS             complement(<1..>2885)
                     /locus_tag="VG754_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HWF19835.1"
                     /translation="RAGSVVLPLHDPIRVAEEWSVVDNLSHGRVGLSFASGWHSNDFV
                     FAPENYADRKKVMFQQIETVLKLWRGETVACPGPDGKEVKVKILPRPVQPNVPVWITA
                     SASPDTFRQAGEMGANVLTNLLGQTIEEVAGKIAIYRQAWREHGHGPGEGHVTLMLHT
                     FVDEDMKTVREKVRGPFTEYLKTSMDLVRKAASAWSFAAFQQPASARSSSSESAQADF
                     SKLSPADMEALLEHAFERYFETSGLFGTPASCMKRVNELRRVGVDEIACLIDFGVDVD
                     SVLASLNFLEELNRKSNDNSAGKRANYSVPAQIQRHHVTHLQCTPSLARMLISDPEGA
                     TAFGALQEFLVGGEALPPSLASQLLGIVRGRIHNMYGPTETAIWSTTKLLTGTSGDVT
                     IGRPLANTEIYVRDKYDQPAPVGLPGELLIGGEGVARGYLNRPELTAERFIAHPFCSE
                     PVARLYRTGDLARYRENGEIEFLGRLDHQIKLRGHRIELGEIEAALGGHPAVKESVVV
                     ANEDASGNARLVAYIVPANRSSAGTETGSPETKDRLAQWQMIWDGAYNEGNPTPDAAF
                     NIAGWNSSYTGQPVPDAEMREWVERTAERIVALRPANALEIGCGTGLLLFRLAPHCKQ
                     YIGCDFSERAIEYLRQQSVQLPQVTLLQKTADDLAGVPAESLDTVILNSVAQYFPNID
                     YLVRVLEGAVKAVSPGGRIFIGDVRNLKLLEAFHASIQMQQAPSELTRNQFRQRVQGQ
                     VAREEELVIAPEFFHALRKYLPRITHVQIQLKPGRFHNEVTRFRYDVTLHVDKAVQPE
                     ANIVWLDNAPESLTPGIITEALSQNGSGYAGIRRVTNARLVNENLALAWMSGSEGPET
                     LGELRAAAQAERKVNAVDPENVLTLAREAGCAVDIIWTDDADDTGAFDVIFRRSELPS
                     PTISSRNISSRRKAWTEFANQPAKGRPDTKLVNQLRTHLKQRLPAIMV"
BASE COUNT          629 a          872 c          738 g          646 t
ORIGIN      
        1 accatgattg ccggcaatct ttgtttcaga tgagtgcgca attgattgac aagctttgtg
       61 tcaggccggc ctttggcagg ctggttcgca aattccgtcc aagccttgcg ccgtgacgaa
      121 atgtttcgtg aggaaatggt cggtgatggc aattctgaac gccggaaaat aacgtcgaac
      181 gccccggtgt catccgcgtc gtccgtccaa atgatgtcaa cggcacaacc ggcttcgcgc
      241 gccagcgtga gaacattttc gggatcaacg gcattaactt tccgttcggc ctgcgcagca
      301 gcgcgcaatt cacctaaagt ttcaggtcct tcactgccgg acatccatgc caaagcgagg
      361 ttttcgttta caagccgcgc attagttacg cgccgaatac ctgcgtaacc ggaaccattt
      421 tgggacaatg cctcagtaat gatgccgggc gttaaactct caggggcgtt gtccaaccag
      481 actatattcg cttccggctg gacggcttta tcaacgtgca gcgtgacatc ataacgaaag
      541 cgcgtcactt cgttgtggaa acgaccgggc ttgagctgga tttggacatg cgtgatgcgc
      601 ggcagatatt tccgaagcgc gtggaaaaat tccggcgcga tgaccaactc ctcctcgcgc
      661 gccacttgtc cctggactcg ctgacgaaat tggtttcgcg taagttcaga cggcgcttgt
      721 tgcatttgaa tcgacgcatg aaaagcttcg agcaatttaa gattgcgcac atcgccgatg
      781 aaaattcttc cgcccggcga cactgccttg accgcgccct ccagcacgcg cacgagatag
      841 tcaatgttgg gaaaatattg cgcgacggaa ttcaggatca ccgtgtccaa cgactcagcc
      901 ggaacacccg ccaaatcgtc cgccgttttt tgtaaaagag ttacctgcgg caactgaacc
      961 gattgctgcc gcaggtactc gatggccctt tctgagaaat cgcagccaat gtattgcttg
     1021 caatgcggcg cgaggcggaa caacagcagt cccgtgccgc atccgatctc gagcgcattt
     1081 gccggtcgca acgcaacaat gcgctccgca gtgcgttcaa cccattcgcg catctccgcg
     1141 tcgggcaccg gttggccggt gtaactgctg ttccagcccg caatgttgaa ggcggcatct
     1201 ggtgtcgggt tgccttcgtt gtaagccccg tcccaaatca tctgccattg cgcgagccgg
     1261 tctttcgttt ccggcgagcc agtttcggtt cccgccgaag aacgattcgc cggcacaatg
     1321 taagccacca aacgcgcgtt gcccgatgca tcttcattcg caactacaac actctccttc
     1381 accgccggat gcccgcccaa cgccgcttca atctcgccca gttcaatgcg atggccgcgc
     1441 aatttgattt ggtgatccaa acgcccaagg aattcaatct cgccattctc gcgataacgc
     1501 gccaaatcgc cggtgcgata aagccgcgct accggctccg agcagaaggg gtgagcgata
     1561 aaccgctccg ccgtcaattc cggacggttc aaataaccgc gcgcaacgcc ttcgccgccg
     1621 atcaataatt cccccggcaa tccgactggc gcaggctggt catatttgtc gcgcacataa
     1681 atttccgtgt tcgccagcgg acggccaatc gtcacgtccc cgctcgttcc ggtgagcaat
     1741 tttgtcgtgg accagatggc ggtttcggtc gggccataca tgttgtggat gcgtccgcgc
     1801 actattccga gcagttgcga agcaagagac ggcggcaacg cttcgccgcc caccaaaaat
     1861 tcttgcaacg cgccaaatgc cgttgcgcct tccggatctg aaatcaacat ccgcgccagc
     1921 gatggcgtgc attgcagatg agtcacgtga tggcgctgga tttgcgcggg aacggaataa
     1981 ttcgcacgct ttcctgcgga gttgtcgttg gacttacgat tcaattcctc caaaaaattc
     2041 aaactcgcca gcacagaatc cacgtccacg ccgaaatcaa tcaggcaggc gatttcatcc
     2101 acgccgacgc gccgcaattc gttcacgcgc ttcatgcaac tcgccggcgt gccaaacaaa
     2161 ccgctggttt caaaatagcg ttcaaatgca tgctccagca gcgcttccat gtcggctgga
     2221 ctcagtttgc taaaatctgc ctgcgcggat tccgagctgg aagaacgcgc cgatgctggt
     2281 tgttgaaatg cagcgaagct ccatgccgat gcggctttgc gaaccaaatc cattgaagtt
     2341 ttcaaatatt ctgtgaacgg tccccgcact ttttcgcgca ccgttttcat gtcctcatct
     2401 acaaacgtat gcaacatcaa cgtgacatgc ccttcgcctg ggccgtggcc atgctcacgc
     2461 caggcttggc ggtaaatggc aatctttccg gcaacttctt cgatggtttg tcccagcaga
     2521 ttggtcagca cgttcgcccc catttcaccg gcttgccgga acgtatccgg actcgctgaa
     2581 gcggtgatcc acaccggaac attcggctgc accggacgcg gcaaaatttt taccttcact
     2641 tcctttccgt ccggacctgg gcaggcaacc gtttcgccgc gccacaattt caacaccgtc
     2701 tcaatctgtt gaaacatcac ttttttccgg tcggcgtaat tttccggcgc gaatacgaaa
     2761 tcattcgaat gccagccgga tgcgaatgaa agccccacgc gcccgtgcga caaattgtcc
     2821 acgactgacc attcctcggc gacgcggatg ggatcgtgca atggcagcac cacgcttccg
     2881 gcgcg
//
LOCUS       DASZMM010000468         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1008314, whole genome shotgun sequence.
ACCESSION   DASZMM010000468 DASZMM010000000
VERSION     DASZMM010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008314"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VG754_11240"
     CDS             complement(<1..843)
                     /locus_tag="VG754_11240"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWF19836.1"
                     /translation="MKVYDNPTFQMACKQFDTVADRLEIPEDERARLKYPKRSMTVAL
                     PIHRDDGSTEVFAGYRVQHHLTLGPTKGGLRYHPDVTLGEVAALAMWMSWKCALTGLP
                     YGGAKGGITCDPRKLSTAELERLTRRYTQEMIPFIGPQVDVMAPDLGTNEQVMAWMMD
                     TYSMHMGHSVPSIVTGKPVLLGGSLGRREATGRGVAYLINRAADTIGLDVGKATAVVQ
                     GFGNVGSVAALSMMRYGIKVIAVSDAFGGVFNAKGLDLWKLDQFANENKTVVGFPEAE
                     PISNE"
     gene            946..1680
                     /locus_tag="VG754_11245"
     CDS             946..1680
                     /locus_tag="VG754_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF19837.1"
                     /translation="MSDTLVVNEIYLSLQGESTFAGLPCIFVRLTACNLRCSYCDTAY
                     AFTEGKKQSLADMLAEIHRQAASFSQRIDGHKMPLIELTGGEPLLQPNALPLMKTLCD
                     EDFTVLIETSGAHDISRVDPRVRRIMDLKCPSSGEVARNRWENLAHLKATDEIKFVIG
                     TREDYEWAKEKIMEHKLAAICPLLFSWVQPLAPEQQDPSLKKVPTGQTPISRRELAER
                     IIADALPVRFQAQLHKIIWPAEQRGV"
     gene            1681..>2465
                     /locus_tag="VG754_11250"
     CDS             1681..>2465
                     /locus_tag="VG754_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWF19838.1"
                     /translation="MTKTQKILQQLREYESRNVTFIEADGSWPVVWERARGAHVWDAE
                     GRKYLDLTAAFGVAAAGHANPRVVRAGQKQMQNLLHAMGDVHPHALKAKLARELSRLT
                     FERWSAAKFKVQGSKFKAGKTIFCNSGFEAVEAALKTAMLATSKSGVIAFEGAYHGLG
                     YGTLNATHREHFRGPFMKQIGGFAEFVGFPTTLRQMKRSLSEIRDLLHYKEIGAVLVE
                     PVQARGGINVPPQTFLPALRKLCDEYGAVLVLDEIYTGFGRTG"
BASE COUNT          550 a          677 c          723 g          515 t
ORIGIN      
        1 ctcgttggag atcggctcgg cttcgggaaa gccgacaaca gttttatttt cgttggcgaa
       61 ttggtcgagt ttccagaggt caagtccctt ggcgttgaac accccgccga aagcgtcgct
      121 gaccgcgatg acctttattc cgtaacgcat catcgaaagc gccgcgacag agccgacatt
      181 cccgaaacct tgcacgaccg cagtcgcttt gccgacatcc aaaccgatgg tatcggcggc
      241 gcggttgatg aggtaggcaa cgccgcgtcc cgtggcttcg cgacggccca gagaaccgcc
      301 gagcagcacc ggcttgccgg tgacgatgct gggaacgcta tgccccatgt gcatcgagta
      361 agtgtccatc atccacgcca tgacctgttc gttggtgcca aggtctggcg ccatgacatc
      421 cacttgcggc ccgatgaacg gaatcatttc ctgcgtataa cggcgggtaa ggcgttcaag
      481 ttcggcagtg gacaattttc ggggatcgca agtgatgccg ccttttgcac cgccgtacgg
      541 caagccggta agcgcgcatt tccaactcat ccacatcgcc agcgcggcca cttcgccgag
      601 cgtcacgtcc ggatgataac gcaatccgcc cttggtcggg ccgagcgtca aatgatgttg
      661 cacccggtaa ccggcgaaaa cttctgtcga accgtcatcg cggtgaatgg gcagcgcgac
      721 ggtcatggag cgctttggat atttgagcct ggcgcgttcg tcttcgggaa tctcaagccg
      781 gtctgccacg gtatcgaact gcttgcaggc catttggaag gtcggattgt cgtacacttt
      841 catcgccaaa aaaatagcgt gaaacggatg gcagggcaac ttgcaaggtt tgccgtcatt
      901 gaattggcta tttcgtcatt ggacattttc ccttacatta catccgtgtc cgatacgctt
      961 gtcgtcaacg aaatttactt gagcctgcaa ggcgaaagca ccttcgcggg tttgccatgc
     1021 atttttgtgc ggctcaccgc gtgcaatttg cgctgctcct actgcgacac tgcttacgcg
     1081 ttcaccgaag gaaagaaaca gtcgttggcg gacatgctgg cagagattca ccgtcaagcc
     1141 gcgtccttca gccaacgcat tgacggccat aaaatgccct tgatcgaatt gaccggcggc
     1201 gaaccgttac ttcagccaaa tgctttgccg ctcatgaaaa ctttatgcga cgaagacttc
     1261 accgtgctca tcgagacgag cggcgcgcat gatatttcgc gcgttgaccc gcgtgtgcgg
     1321 cgcatcatgg atctgaaatg tccgagcagc ggcgaagttg cgcgcaatcg ctgggaaaac
     1381 ctggcgcatt tgaaagcgac cgacgaaatc aaattcgtca tcggcacgcg cgaagattac
     1441 gaatgggcga aagaaaaaat catggaacac aaactggccg cgatttgtcc gttgctcttt
     1501 tcatgggtgc aaccgctcgc gccggagcaa caggacccgt cactgaaaaa agttccaacg
     1561 ggacaaacgc ccatttcgcg gcgcgaactg gcggaacgga tcatcgcgga cgcgttgccg
     1621 gtgcgttttc aggcgcagtt gcacaaaatc atctggcccg cggaacaacg aggagtttag
     1681 ttgacgaaga cacaaaagat tcttcagcaa ctgcgcgaat acgaatcgcg caacgtcact
     1741 ttcattgagg cggacggctc ctggccggtg gtttgggaac gcgctcgcgg cgcgcacgtc
     1801 tgggacgcgg aaggtcgcaa gtatctggat ttgacggcgg ctttcggcgt cgcggctgcg
     1861 ggccatgcca atccgcgggt cgtgcgagcc ggccagaaac aaatgcagaa tttattgcac
     1921 gcaatgggcg atgtgcatcc gcacgcgctc aaggcgaagc ttgcgcgcga actcagccgc
     1981 cttacgtttg aaagatggag cgcggccaag ttcaaagttc aaggttcaaa gttcaaagct
     2041 ggcaaaacca ttttctgcaa ttcgggattt gaagcggtcg aagccgcgtt gaagacggcg
     2101 atgcttgcga cgagcaagtc gggagtgatc gcgtttgagg gcgcgtatca cgggctgggt
     2161 tacggcacac tgaacgccac gcatcgcgaa cattttcgcg ggccgttcat gaaacagatt
     2221 ggcgggttcg cggaattcgt cggcttccca acgacgctgc ggcaaatgaa gcgcagcctg
     2281 tccgagatcc gcgatttgct gcattacaaa gaaattggcg cggtgctggt cgagccggtg
     2341 caggcgcgtg gaggaatcaa cgtgccaccg caaacatttc tgccggcgtt gcggaaattg
     2401 tgcgacgaat acggggctgt gctggtgctc gacgaaattt acaccgggtt cggccgcacc
     2461 ggaaa
//
LOCUS       DASZMM010000469         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3501627, whole genome shotgun sequence.
ACCESSION   DASZMM010000469 DASZMM010000000
VERSION     DASZMM010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501627"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VG754_11255"
     CDS             complement(<1..315)
                     /locus_tag="VG754_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWF19839.1"
                     /translation="MVCKAILCLSAAICLVSCTPHRTESVSGTIETDEVHVGSRYGGR
                     VETIFAGEGDSLKAGELIVQLEDSELQARLDRANAVLQELMAGPRVQELATAKSEWEA
                     QVA"
     gene            complement(363..1070)
                     /locus_tag="VG754_11260"
     CDS             complement(363..1070)
                     /locus_tag="VG754_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19840.1"
                     /translation="MIQPNCRVQFTAEDVAFISTVLATKGGSAPSLVSLLTDSEARDL
                     ILDDDDLFRALLEQRRCLRVSAHFYFYVLVRHVFRRSGIEDRTVADYVAEVLSEFSHQ
                     QNTRCVVPGSDKPLDYLFEMLAALQTADDRMSFYIRAHIGNQSLFMSGVFPDRIRARS
                     ERRGFPNLRYYEDLGRANFRAASGHRLARKFDLAPIFEQLSVRFESTRMALNDLSDRI
                     FSISDPELPVSLLKSLS"
     gene            1329..2195
                     /locus_tag="VG754_11265"
     CDS             1329..2195
                     /locus_tag="VG754_11265"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWF19841.1"
                     /translation="MNTEPAFIPARSRRQVMDWGLVLASQGIDAAIVREQDTWALSVS
                     SADYERACAMLNQYVVENRGWKWQQSLPGSGLFFHWGSLLWALAVGAIYYWSMVLFPQ
                     MKMAGVMDSQLIREGQWWRLFTAVTLHADMSHLLANMTTGIILLGFAMARFGPGPAVL
                     ATYIAGVLGNVADLFLYHDEHRSLGASGMVMAALGILAVQSFSLWHKDHSFRQLIARA
                     FVAALLILILIGTSPESDVVAHFGGFFAGALFGYALSWINPDGLKLFAVNFVTIVITM
                     FLVAITWWKAKG"
     gene            complement(2272..>3065)
                     /locus_tag="VG754_11270"
     CDS             complement(2272..>3065)
                     /locus_tag="VG754_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF19842.1"
                     /translation="ERNLHRRLIRTGNGDEMDRLTEVFNSMVARLENSFQQIREFSLH
                     ASHELKTPLTVMRGELETALVATNHSDEQRERLLSELDEVLRLTKIVDGLTLLTKADA
                     GQISLAQDDVQLDELVRDCFEDAKILAQARDVHVCLGKVEQVSIKGDRHRLRQLLLNL
                     SDNAVKYNIINGVVNLRLRMVDGMAELEIANSGPGIAPELQLRVFNRFFRGDSSHNNS
                     VEGCGLGLSISQWIVHAHGGSIEFRSKPEPMTTFTVRLPTQRAAA"
BASE COUNT          720 a          956 c          709 g          680 t
ORIGIN      
        1 cgcaacctgc gcttcccatt cgcttttggc cgtcgcaagc tcctgcactc gcgggccggc
       61 catcaattcc tggagcaccg cattggcgcg atcgagccgc gcctgcaact cactgtcttc
      121 aagctgcaca atcaactcac ctgccttgag agaatcgcct tcgcccgcaa aaatcgtttc
      181 cacgcgtccg ccgtaccgcg accccacatg aacctcatcc gtctcaatcg tgcccgaaac
      241 ggattcagtg cggtgcggcg tgcacgaaac gaggcaaata gcagcgctca ggcagaggat
      301 ggctttacac accattacta cctgatgcca cgaatctgga aaaaatacag gacgatttgg
      361 gtttagctca gggatttcaa caagctaaca ggcagttcag gatcgctaat cgagaaaatc
      421 cggtccgaca aatcgttcaa cgccatccgc gtgctttcaa atcgcaccga caattgttcg
      481 aagatcggcg caagatcaaa ttttcgcgcc agccgatgtc cgcttgccgc gcgaaaattc
      541 gctcggccaa ggtcttcata gtaacgcaag tttgggaacc cgcgccgttc actgcgcgcg
      601 cgaatgcgat cgggaaacac accagacatg aacaacgatt gattcccaat gtgcgcccga
      661 atgtaaaaac tcatccggtc atccgccgtt tgcaacgcgg cgagcatttc gaacaaataa
      721 tccaaaggct tgtcgctccc aggcacaacg caacgcgtat tttgctggtg cgaaaattca
      781 ctcaacactt ccgccacata atcagcaacc gtgcgatctt ctattcccga ccgccggaac
      841 acatgccgca ccagcacgta aaaataaaaa tgcgccgaca cacgaaggca tcggcgctgt
      901 tccagcaaag cccggaacaa atcgtcgtcg tctaagatta gatcacgcgc ctctgaatca
      961 gtcaggagag aaaccagcga aggggcagaa ccgcctttgg tcgcgaggac cgttgagata
     1021 aacgccacgt cttccgccgt aaattgcacc cggcaattag gctgaatcat tttcataaca
     1081 ctgcaatcag caacttcaat tccaagtaat tggctggaag aatatcatcc atatgcgccg
     1141 cgaaaatgaa aggaaaatcc gttccgaact cactttttta cactcacaac cactttcctc
     1201 actcctcttt gctataaaat aaactcctcg cctccgctct tcaacaagga gccgcgcatg
     1261 cgtaagcgcc cttatattcg acaccttgca attgcatcct ttcttccgag ccgccaaact
     1321 tttggaaaat gaacaccgaa cccgctttca ttcccgcgcg ctcgcgacgg caagtgatgg
     1381 attggggttt ggtgctcgcc agccagggca tcgacgccgc gatcgtgcgc gaacaagaca
     1441 cctgggctct ctccgtttcg tccgccgact acgaacgcgc ctgcgccatg ttaaatcaat
     1501 acgtcgtcga aaatcgcggt tggaaatggc agcaaagcct gcccggctcc ggcttgttct
     1561 tccactgggg cagcttgctg tgggcgctcg ccgtcggcgc gatctactac tggagcatgg
     1621 tcctgtttcc acaaatgaaa atggcgggag taatggacag ccaactcatc cgcgagggcc
     1681 agtggtggcg gcttttcacc gcggtcacac ttcacgccga catgagccat ctccttgcga
     1741 acatgaccac cggcattatt ctcctcggct ttgccatggc gcgattcggc cctggtccgg
     1801 cggtgctcgc gacctatatc gctggcgttc tcggcaacgt cgccgattta tttttgtacc
     1861 atgacgaaca ccgctcgctc ggcgcatcag gcatggtcat ggccgccctt ggaattctcg
     1921 ccgtccaatc attttcgctt tggcacaagg accacagctt tcgccaactc atcgcccgcg
     1981 catttgtcgc cgcgctcctg atcttaatat tgattggaac atccccggag agcgacgtcg
     2041 tcgcgcattt cggtggattc ttcgccggcg ccctcttcgg ctatgcgttg agttggataa
     2101 accccgatgg attgaagctc ttcgcggtaa attttgtcac tatcgtcatc acgatgtttc
     2161 tcgtcgccat tacctggtgg aaagccaagg ggtaaaattc acgggctcaa taacgtttca
     2221 atcagcaaga ccctgccgaa ttgctgcgac gatacaccgc cgactttccg actaagcagc
     2281 cgcacgttga gtcggcagtc gcaccgtaaa cgtcgtcatc ggctcgggct tcgaacggaa
     2341 ttcgatactt ccgccatgcg cgtggacaat ccattgggaa atgctcaacc ccaggccgca
     2401 accctccacc gaattgttgt gactcgaatc gccacgaaaa aaccggttga acacccgcag
     2461 ttgcaattct ggcgcaatgc ccggacccga attggcaatc tcaagctcgg ccattccgtc
     2521 aaccatccgc agccgcagat tcacgactcc gttgataata ttgtacttca ccgcgttatc
     2581 cgaaaggttc aataacaact gcctcaaccg atggcgatcc cctttgatgc tcacctgctc
     2641 taccttgccc aaacagacgt gcacgtctcg cgcttgcgcc aaaatcttcg catcctcaaa
     2701 acaatcacgc accaattcat ctaactgcac gtcgtcctgc gcgaggctga tctggcctgc
     2761 gtccgccttc gtgagaagcg tcaggccatc cacaatcttc gtcaggcgca acacctcatc
     2821 caattcgctc aatagccgtt cgcgctgctc gtcgctatga tttgtcgcca ccagcgccgt
     2881 ctccaattcg ccacgcatca ccgtcagtgg agttttcaat tcatgcgacg cgtgcaatga
     2941 aaattcgcga atctgctgaa atgaattctc caaacgtgcc accatcgagt tgaaaacctc
     3001 cgtcaaccgg tccatctcat cgccgttacc agtgcgaatc agtcgtctgt gcaaattgcg
     3061 ttcgt
//
LOCUS       DASZMM010000470         5499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_193394, whole genome shotgun sequence.
ACCESSION   DASZMM010000470 DASZMM010000000
VERSION     DASZMM010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193394"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VG754_11275"
     CDS             <1..535
                     /locus_tag="VG754_11275"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19843.1"
                     /translation="AILRMRNMRLPEQEKRLGVICKLRNFHRAAIDMTGIGLGLCEYA
                     QQSFGSSRIRGINFASSVPVRHAVPEGELHPNVRVTELLATELLRVYEDRRIQHPREE
                     RLRADLRKPEKVTSPGGRVSIAATRDSSGHADHFWSLALAIEAGGGSKVSNRCGMVTF
                     KNRPKWWWESRARFLGF"
     gene            1098..1664
                     /locus_tag="VG754_11280"
     CDS             1098..1664
                     /locus_tag="VG754_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19844.1"
                     /translation="MKKMRSNAGWNTFTPQQKEAIEEWLLVQNLSYKAVLKRCAEELG
                     LKWSLTAICRTYKHLAAARAVRDTMAMDKTSEELIEKGKPAERLRPSIMKMAATRLLE
                     KAIENSDTQDMARYGRLLMQGEAQQIQRDRAALAAKRHEFNASQAALKALPFVNDFTH
                     ADLIREKARTAAIRLILFGNAPKKGVAK"
     gene            1892..3166
                     /locus_tag="VG754_11285"
     CDS             1892..3166
                     /locus_tag="VG754_11285"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWF19845.1"
                     /translation="MNNELAIKGGAKAVTNKLQGWPQFDEPAIKAVEDVLRSGKVNYW
                     TGKRGMEFEKKFAEWQGSKHAISVATGTAALHVALNSLGIGPGDEVIVPSYTFIATSF
                     SVVQAGAIPRFADVNLEDHCISVESAEKLVNERTKAIMPVHLYGNVCDMDKIKAFAKK
                     HKLFVIEDNAEAFGGTYKGKKTGTLTEIAGCSFCQNKTFTTGGEGGMITTDDEDLAWT
                     ARSFRDHGYDVKQRLSLLELEQKLPYIHNMVGWNYRMTEMQSAIGLAELDRMDNWNMP
                     RRRRNAQILVNALKTSKLIKYFPVNTLERQNGWYVLAFSLDVENMNCDIKEFVKAASA
                     EGAPVWKVFWPQCHTERAFQEGRAFGRSGFPFKSKEYADPASVDYTHVDVPNARWHEA
                     HTFTCFAYPTFTESDMEQIAEAILKVINAYQK"
     gene            3176..4237
                     /locus_tag="VG754_11290"
     CDS             3176..4237
                     /locus_tag="VG754_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19846.1"
                     /translation="MRDKVKWGVIGSGGIARRRTIPEGFVPATNARLVSVYGTNATTN
                     HEVAKQFGAQPCDSLKALLDSDIEAVYVASPVQAHHEQVLACARAGKHVLCEKPLGRT
                     VAEAEEMAAACQRAGVQFGTAFMMRFQTQHQAAAEIIRAGKLGQPVYARAQLSCWYPP
                     LASSWRQDPSLGGGGSLMDLGGHCIDLLEMFFGPVKRISCFTKSLVHAYASEDSAATL
                     LQFVNGALATVDTFFCIQDSSSKNALELYGSKGSIHATGTIGQGSQGEMTCQLLSGET
                     GYEAQQARNASGGVKISPEPQNIYRAEIEAFSKGLLESRPDPEQSAELGLHSQRLLAA
                     CYESARSGQAVTIGQDNSV"
     gene            complement(4234..5364)
                     /locus_tag="VG754_11295"
     CDS             complement(4234..5364)
                     /locus_tag="VG754_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HWF19847.1"
                     /translation="MTTTAEAKAMNVPPAATAARPRFISVDALRGFDMFWILGGDQAV
                     RALKTIHDSPLMRGLAVQMEHCEWAGFHFYDFIFPLFVFIVGVSLVFSISRMLEREGR
                     AAAIKRIAVRSIILFLLGVFYMGGIADGFDKMYFAGVLHRIAVAYFFAALIFCFFRLR
                     TMIFICAGLLVVYWALITFVPVPGIGAPSLAEPGRNLAHYLDGRFLPGQRFEGTLLST
                     LAAVANCLIGVFAGLLLKNQNISEPGKVRWLALGGIAALAAGYIWSLQFPIIKLLWTS
                     SYVLVACGYSALLLALFHQVIAVWQFQKWARPFVWIGMNALTIYIITNVVSFHRLALR
                     LVGGDISHFLGAWSELVTALTALALIFWLAYFLYRKQIFLRL"
BASE COUNT         1365 a         1471 c         1588 g         1075 t
ORIGIN      
        1 ggccatcttg cggatgcgca acatgcgttt gccggagcag gaaaaacgcc tgggcgtcat
       61 ctgcaagctg cgcaattttc atcgcgcggc cattgacatg accgggattg gccttggatt
      121 gtgcgagtat gcgcaacaat ccttcggttc gtcgcgcatt cgcggcatca acttcgcttc
      181 cagcgtgccg gtgcgtcatg ccgttccgga gggcgagctt catcccaatg tccgcgtgac
      241 cgagttgttg gcgacggagt tgttgcgcgt ttacgaagac cggcgcatcc agcatccgcg
      301 cgaggagcgg ttgcgcgccg atttgcgcaa gcccgaaaag gtgacgtcgc ccggcggcag
      361 ggtgagcatc gccgcgacac gcgacagctc gggtcatgcg gaccattttt ggagcctcgc
      421 gctggccatc gaagccggcg gcggttctaa agtctccaat cgctgcggca tggtgacgtt
      481 caagaacagg ccgaagtggt ggtgggaaag ccgggcgcgg tttttgggtt tttaagcctc
      541 cattcaaaag ctgacaatcc catgaaaggg cgaacaaaat cgtactttgg aatattaaat
      601 cgttccggtt gcaatcgcca tttgtatgcg aatcgcgaag gggcgtatct caatttcttg
      661 cacgatggag tgatcggaca tttccttccc cctcacccca gcccaatggc ttgccaagcc
      721 cgccacaagt cagtttcccc aaaggagacg aacgcttcgc gttacgtggt cccttgggga
      781 gagggagcag cgtatcccgt cgtggttgca aaccggcggc gttcaacttt tcggcagcgg
      841 ccgaatggtt ccccctctcc ttggggagag ggccggggtg agggggaccg cggcgtgtgc
      901 cgatccataa acacaatccg caagggtcca gacacgtgca aaatagtgat atgcggcttc
      961 tcgaagcgga agctgaaacc attgaatcaa atcacatcaa gaagcggcaa gcagtcctcc
     1021 ctcgcccgag ggcgggcgta aacaacagac cgccagtttt caattccaca aaaaaaatga
     1081 gtcaacaaaa actacttatg aaaaaaatgc gatcgaatgc cggttggaac accttcacac
     1141 cgcaacaaaa agaagccatc gaagaatggt tgctggtcca gaacctcagt tacaaggccg
     1201 tcctcaaacg gtgcgccgag gagctgggat tgaaatggtc gctgacggcg atatgccgga
     1261 cttacaagca tctcgccgct gcgcgggctg tcagggacac gatggcgatg gacaagactt
     1321 cggaggaatt gattgaaaag ggcaagccgg cggaacggtt gcgtccatcc atcatgaaaa
     1381 tggccgcgac gcggttgttg gaaaaggcga ttgaaaacag cgacacgcag gacatggcgc
     1441 gttacggacg gttgttgatg cagggcgagg cgcagcagat ccagcgcgac cgggcggcgc
     1501 tcgccgccaa acggcatgag ttcaacgcgt cccaagcggc attgaaggcg ctgccgttcg
     1561 tgaatgactt cacgcacgcg gacctgatcc gtgaaaaggc gcgcaccgcg gcgatacggc
     1621 tcatcctgtt cggcaacgcc ccgaagaaag gagtggcgaa gtgaggacgt tccgcttgaa
     1681 acacgcggcg atgcggagaa atttccgctt aattccgctt gtttccaccc cgttccgctt
     1741 gaatccgcat aaaaaaatta tttttttcag gcaaacatct gtctccagtc ttcaaatagt
     1801 cttgacggaa atcagacggt gcgttgcgat aagtcaatgc aatcgagtta ctcaagtaat
     1861 tgctgtctcc acccttaaaa gaaatatcca catgaacaac gaactcgcaa tcaaaggcgg
     1921 cgcaaaggcc gtgaccaaca aactccaggg ctggccgcag ttcgacgagc cggccatcaa
     1981 ggcggtcgaa gatgtcctgc gctcgggcaa ggtcaattac tggaccggca agcgcggcat
     2041 ggaatttgag aagaaattcg ccgaatggca gggcagcaag cacgccatca gcgtcgccac
     2101 cgggactgcg gcgttgcacg tcgcgttgaa ttcgttgggc atcgggccgg gcgatgaagt
     2161 catcgtgccg agttacacgt ttatcgcgac gagtttctcc gtcgttcaag ccggcgcgat
     2221 tccccgtttc gcggatgtga atctggagga ccattgcatc agtgttgaat ctgccgagaa
     2281 gctggtgaac gagcgcacca aggcgatcat gccggtgcat ctctacggca atgtttgcga
     2341 catggacaag atcaaggcat tcgcgaagaa gcataaattg tttgtgatcg aggacaatgc
     2401 cgaggcgttc ggcggcactt acaaggggaa gaagaccggg acgttgactg aaatcgccgg
     2461 gtgcagtttt tgccagaaca agactttcac caccggcggc gaaggcggaa tgataacgac
     2521 cgatgatgaa gaccttgcct ggacggcgcg cagttttcgc gatcatggct atgacgtgaa
     2581 gcagcgtctc agccttttgg aattggagca gaagcttcct tacatccaca acatggttgg
     2641 ctggaattat cgcatgaccg agatgcaatc ggccatcggc cttgcggagc ttgaccgcat
     2701 ggacaattgg aacatgccgc gccgccgtcg caacgcgcag attttggtga acgcgctcaa
     2761 gacttcgaag ttgatcaaat acttcccggt caacacgctc gaacgccaga acggttggta
     2821 tgtgctggcg ttttcactgg acgtcgagaa catgaattgc gacatcaagg aattcgtgaa
     2881 ggcggcgagc gcggaaggcg cgccagtctg gaaagtcttt tggccgcaat gccacaccga
     2941 gcgcgcgttc caggaaggcc gcgcttttgg ccgcagcggt tttccgttca agtcgaagga
     3001 atacgcagac ccggccagcg tggattatac gcatgtggac gttcccaacg cgcgctggca
     3061 cgaggcgcac accttcacct gttttgcgta ccccaccttc acggagtccg acatggagca
     3121 gatcgccgaa gcaatcttga aggtcatcaa tgcgtatcag aaatgatttt tatctatgcg
     3181 cgataaagta aaatggggcg tgatcggttc aggcggaatc gcgcggcggc ggacgattcc
     3241 ggaagggttc gtcccggcca cgaatgcgag actggtttcc gtttacggaa cgaacgccac
     3301 gaccaatcac gaagttgcca aacaatttgg cgcgcaacct tgcgacagtc ttaaagcatt
     3361 gctcgattcg gacatcgaag cggtttacgt ggcgagtccg gttcaggcgc atcacgaaca
     3421 ggtgttggct tgcgcccgcg ctggaaaaca tgttctttgc gaaaagccgc tgggcagaac
     3481 ggttgccgag gcggaagaaa tggccgctgc gtgtcaacgc gctggcgtgc aattcggcac
     3541 ggcgttcatg atgcgtttcc agacgcagca tcaagcggcg gcggaaatca ttcgcgctgg
     3601 gaagttgggc cagccggttt atgcgcgcgc gcagctttcg tgctggtatc cgccgctggc
     3661 aagctcgtgg cggcaggacc cttcgctcgg tggcggcggt tcgttgatgg atttgggcgg
     3721 gcattgcatt gacttgctcg aaatgttttt tggcccggtg aaacggattt cctgtttcac
     3781 caagagcctc gtccatgcgt atgcgagcga ggacagcgcg gcgacgttgc ttcagtttgt
     3841 gaacggcgcg ttggcaaccg tggacacatt tttttgcatc caggacagca gcagcaagaa
     3901 cgcgctcgaa ctttacggct ccaagggaag catccacgcg accggcacga tcggccaggg
     3961 cagccagggc gagatgacgt gtcaattgtt gtctggcgaa acgggctacg aagcgcaaca
     4021 agcgaggaac gcatcaggcg gcgtcaaaat ttcgcccgag ccgcagaata tttatcgcgc
     4081 ggaaatcgag gccttcagca agggcttact tgaaagccgt cccgatccgg agcagagcgc
     4141 agaactcggt ttgcacagcc agagattgct cgcggcctgt tatgaatcag cccgcagcgg
     4201 ccaggctgtg actatcgggc aggataattc tgtttaaagc cgcaagaaga tttgcttgcg
     4261 gtaaagaaaa taggccagcc aaaaaatgag cgccagcgca gtcaacgcag tgaccagttc
     4321 gctccacgcg ccaaggaagt ggctgatgtc gccaccgacc aaccttaatg ccaaccgatg
     4381 aaaacttacc acgttggtga tgatatagat ggtcaaggcg ttcattccta tccacacgaa
     4441 cggtctcgcc catttttgga attgccacac cgcgatgact tgatgaaaga gcgccagcaa
     4501 cagcgcgctg taaccgcaag ccaccagcac gtaactggaa gtccaaagaa gtttgatgat
     4561 gggaaattgc agcgaccaga tatatcccgc cgcaagcgcg gcgattccgc ccaacgcgag
     4621 ccagcgtacc ttcccgggtt cggaaatgtt ttggttcttc agcagcaaac cggcgaacac
     4681 gccgataagg cagttggcta ctgcggccaa agtgctcaac agcgttcctt caaatcgttg
     4741 gcccggcaaa aaacgtccat cgagataatg agcgagattt cgtccaggtt cggcaagaga
     4801 cggcgcgccg attccgggca ccggaacaaa agttatcaat gcccaataga ccacgagaag
     4861 gccggcacag atgaaaatca tcgttcgaag ccgaaagaaa caaaaaatca gggcggcaaa
     4921 aaaataagca accgcaatgc ggtgcaacac gccggcgaaa tacattttgt caaaaccgtc
     4981 cgcgattccg cccatgtaga aaacgcccag cagaaaaaga atgatggatc ggaccgcgat
     5041 gcgtttaatt gccgcagctc gtccttcgcg ttccagcatc cggctgatcg agaacacgag
     5101 cgaaacaccg acaatgaaaa cgaacagcgg aaagatgaaa tcgtaaaaat gaaaaccggc
     5161 ccactcgcaa tgttccattt gaacggcaag cccgcgcatg agcggcgagt catggattgt
     5221 tttcagcgcg cgaacagcct ggtcgccgcc caaaatccaa aacatgtcaa aaccgcgcaa
     5281 cgcatcaacg gaaataaatc gtggccgggc tgctgttgcg gccgggggaa cattcatagc
     5341 cttcgcttca gcagtggtag tcatcgcgag ttgcttgaga gaacgacatc gaacaactca
     5401 tttagctgcc atgaattcat aaaggtgaac gccaaacggc gaaaagtcat ccgacaacga
     5461 atctccttcc acttgcgcag tgtgatcctc atacaaaac
//
LOCUS       DASZMM010000471         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_497312, whole genome shotgun sequence.
ACCESSION   DASZMM010000471 DASZMM010000000
VERSION     DASZMM010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497312"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..440
                     /locus_tag="VG754_11300"
     CDS             63..440
                     /locus_tag="VG754_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF19848.1"
                     /translation="MGMGNGNKSGFTLVELMIVVAIIGLLAAIAIPNMVHARTQSQTN
                     ACINNLRQIDGAKQEWAMEHPVKGTARPGIAELQPYMGRGSQGSLPTCPLDTQNSWDT
                     SYSAGNLTIVPLCRISPTNHLIM"
     gene            742..3399
                     /gene="mgtA"
                     /locus_tag="VG754_11305"
     CDS             742..3399
                     /gene="mgtA"
                     /locus_tag="VG754_11305"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HWF19849.1"
                     /translation="MLKSLFSKISPFLIVKEAKAGAKKITPNEQRLVDFCALSGEEAQ
                     KRLGTGSTGLSAEKVEEARTEFGPNELKVGDRHGILVEILLRFKNPLVIQLLIIAVVS
                     LATAEGPSDRASAVIVGIMVFLSVGLAYFQERRSGRAVEKLQEMVQTNCIVIRDGKEV
                     EAPMFEIVPGDLVLLHAGSIIPADLRLISAKDFFIGQSALTGESMPVEKNAAPSDLAD
                     KGIIELPNACFQGSNVVSGTARAVVINTGNRTYFGSISEKLAGQRVVTSFDRGIASFT
                     WLMIRFMIVLVSIVFLIDAVKEHNFLKALTFSLSVAVGLTPEMLPMIVTVNLSKGALA
                     MSKKKVIVKKLNSIQNLGAINILCTDKTGTLTQDRIILEKHVDVTNRTSDDVLRYAYM
                     NSYYQTGLRNLLDRSILSHTDLDVERSCKKVDEIPFDFSRKRMSVVIDYQGDHVLICK
                     GAVEEIYKVCDRYQVDEEINPLIDVIKNDLMEEYEALSKDGYRVVAIAYREFPREKQV
                     FSVSDESNLILLGYIAFFDPPKESAAKALEGLAKMGVSTKILTGDNALVTQKVCRDVG
                     LAVEKVITGDQLRAMTDEQFSEAVEKANVFARLSPSQKSDIIQALQKRGHVVGFMGDG
                     INDALALKVSDVGLSVDTAVDVAKEAADIVLLEKSLLVLEEGILEGRKVFGNIIKYIR
                     MGASSNFGNMFSFVGGTAILPFTPMLSPQILTNNLLYDFSQLAIPTDKVDEEYLLRPR
                     KWNIDNVKKFMVYVGPISSIFDYCTFALMWFFYHCSTHSQPHDGAERLFQTGWFVESL
                     LTQTLIVHIIRTGRIPFIQSSPSWQLLMGTLVVMGIGAWLPYSPLANFLDFVPLPGSF
                     WLWMLLFLVSYSVLCHYVKVWFFNKFGTD"
     gene            3402..4235
                     /locus_tag="VG754_11310"
     CDS             3402..4235
                     /locus_tag="VG754_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWF19850.1"
                     /translation="MKNLINALQEGRFVELPETNKEKALNYLANLIEAMPDLPAGTDV
                     AGAVLTREAQFNTGVGQGWACPHMRTSHEGDIICAAGWSPAGIDYGACDHKPVHFVVM
                     YYVPDSQKHVYLKEISGLAKAIQSKQGDKDFSKIQTLAQAREWILDLVSTAVETQMTD
                     VKARMIHLEAKQAAVTSAPGAGGLTLSNLPHVVVPLSILVVPGVRAMVLSQDPDVIAK
                     LESGGEIDTGALAKQMPFDHAGYTVLFRGVTNYQAKRQLYDCLAIKLNGAPPSADKPK
                     V"
BASE COUNT         1025 a         1127 c         1265 g         1002 t
ORIGIN      
        1 gaaatcattg ttgttacaaa aatcatccgc tgcccgccac gatttttggg aagggttgag
       61 ttatgggaat gggaaatgga aacaaatcgg gattcactct tgtcgagctg atgattgtcg
      121 tggccatcat cgggctgctc gccgcaatcg caatccccaa catggtacac gcccgcacac
      181 agtcgcagac caacgcctgc atcaataacc tgcgccagat cgacggtgcc aagcaggaat
      241 gggccatgga gcaccctgtt aaaggcacgg caagaccagg catcgcggaa ttgcaacctt
      301 acatgggacg cggctcgcaa ggttcccttc cgacctgtcc gctcgacacc cagaattcct
      361 gggacaccag ttacagcgcc gggaacctga ccattgttcc tctctgtaga atttctccca
      421 ccaatcatct catcatgtag ttcgcgcatc gtcattgaac gccgtcaaaa gcgggttaca
      481 atgaagaaaa ggttttggcc cttaaaactt tcggcagctt tgttgcgtcg aacggcaaaa
      541 aatgctggtc ttcgcccgcg accccggtta aaagttcggg cgaattggcg gcgaatgtcc
      601 ttccggcatg ggtcggacct tcagcggttt ccggagtaac gaaacccaaa aacgaatctg
      661 cgatcgagat ggaagtaaaa gtgacagagt ggtgtcgtcg gccttcgggc tggcgggact
      721 tttttcacgc gggaggcacc catgcttaaa tccctgttct caaaaatttc ccccttcctg
      781 atcgtgaagg aggcgaaagc gggcgcgaaa aaaatcacgc ccaacgaaca gcgtctggtg
      841 gatttttgcg ctttgagcgg agaggaggcg cagaaacggc tgggaacggg atcgaccggg
      901 ttgagtgcgg aaaaagtgga ggaagcgcga acggaattcg gcccgaacga attgaaggtg
      961 ggcgaccggc atgggatcct tgttgaaatt ttgctgcgat tcaaaaaccc gctcgtcatc
     1021 caattgctca tcatcgcggt ggtttcactg gcgacagcgg aggggccgtc ggaccgcgct
     1081 tcggcggtga tagtgggcat catggttttc ttgagcgtcg ggctggcata ttttcaagag
     1141 cgtcggtcgg gcagggcagt tgagaaattg caggaaatgg tgcagacgaa ttgcatcgtc
     1201 atccgggacg gcaaggaagt cgaagctccc atgtttgaaa tcgtgcccgg cgatttggtg
     1261 ttgctccatg ccggttcaat catcccggct gacttgcggc tgatttcggc gaaggacttt
     1321 ttcatcgggc aatcggcgct caccggtgaa tccatgccgg tcgaaaaaaa tgccgcgccg
     1381 agcgacctgg cggacaaggg catcatcgaa ctgccgaacg cgtgtttcca aggcagcaac
     1441 gtcgtcagcg gcaccgcgcg cgccgttgtc atcaacaccg gcaaccgcac ctatttcggc
     1501 tccatctccg aaaaacttgc cgggcagaga gtcgtcacca gctttgaccg cgggatcgcg
     1561 agtttcacct ggttgatgat ccggttcatg atcgtgctgg tcagcatcgt gttcctgatt
     1621 gacgccgtga aggagcataa tttcctcaag gcgctcacgt tcagcttgtc cgtcgcggtg
     1681 ggcctgacgc ctgaaatgct cccgatgatc gtgacggtga atctttccaa gggcgctttg
     1741 gcgatgtcca aaaagaaggt catcgtcaag aaactcaatt ccatccaaaa ccttggcgcg
     1801 ataaatatcc tttgcaccga caagaccggc acgctcacac aggaccgaat catcctggaa
     1861 aagcatgtgg acgtgaccaa ccgaacaagt gacgacgtgt tgcgatacgc gtacatgaac
     1921 agttattatc aaacggggct gcgcaacctg ctcgaccgct cgattctttc acacacggat
     1981 ttggacgtgg aacgaagctg caagaaggtg gacgaaattc ccttcgattt cagccggaaa
     2041 cggatgtcgg tggtgattga ttatcaaggc gaccacgtgc tgatctgcaa aggggcggtg
     2101 gaggagattt acaaggtttg cgatcgttac caggtggatg aggaaatcaa tccccttatt
     2161 gatgtaatca agaacgattt gatggaggaa tacgaggcgt tgagcaagga cggttatcgc
     2221 gtggtggcga ttgcgtatcg ggaatttcca agggaaaaac aggtcttcag cgtgtcggat
     2281 gaatcgaacc tcattttgct cggctatatc gccttcttcg acccgccgaa ggaatcagcc
     2341 gcaaaggcgc tcgaagggct ggccaaaatg ggcgtgagca caaaaatcct gaccggcgac
     2401 aacgcgcttg tgacacagaa agtttgccgc gacgtgggtt tggccgtgga aaaggtcatc
     2461 acgggggacc aattgcgcgc catgaccgac gagcaatttt ccgaggcggt cgaaaaggcg
     2521 aatgtgttcg cgcggctttc cccttcgcaa aagtcggaca tcatccaggc gctgcagaag
     2581 cgcggtcatg tggtcggttt catgggcgac ggcatcaatg acgcgctggc gttgaaggtt
     2641 tctgatgttg gactttcggt ggacacggcg gtggatgtgg cgaaggaagc tgccgatatt
     2701 gtcctgctgg aaaaaagttt gctcgtgctg gaggaaggga ttttggaagg ccgaaaagtt
     2761 ttcgggaaca tcatcaaata catccggatg ggcgcgagtt ccaatttcgg caatatgttc
     2821 agcttcgtcg gcggcaccgc gattttgcca ttcacgccga tgttgtctcc gcaaatcctg
     2881 acaaacaatc tgctctacga tttttcgcag cttgccattc ccaccgacaa ggtggatgag
     2941 gaatatctgc tgcggccgcg caaatggaac attgataatg tcaaaaaatt catggtttat
     3001 gtcgggccga tcagttccat ttttgattat tgcacgttcg cattgatgtg gttcttttat
     3061 cattgttcga cccattcaca accgcacgat ggggctgaac gattgttcca aacgggctgg
     3121 tttgtcgaat ccttgctgac ccaaacgctg atcgtccaca tcatccgcac cggacggatt
     3181 cccttcatcc aaagctcgcc cagttggcaa ttgttgatgg gcacgctggt tgtgatggga
     3241 atcggcgcgt ggctgcctta ttcgccgctg gcaaatttcc tcgacttcgt tccgctgccc
     3301 ggttcgttct ggttgtggat gttgctattt ctggtcagtt actcggtgtt gtgccactat
     3361 gtgaaggtat ggttttttaa caaatttggc actgactgac catgaagaac ctgatcaatg
     3421 cgttgcagga aggccggttc gtcgaactcc ccgagacgaa caaggagaag gcgctgaatt
     3481 acctggccaa cctgattgaa gcgatgcccg atttgcctgc gggaacagac gtcgcgggcg
     3541 cggtgctcac gcgcgaagcg caattcaaca ccggcgtcgg ccagggttgg gcctgtcccc
     3601 acatgcgcac ttcgcacgaa ggcgacatca tctgcgcggc tggctggagc cccgcgggaa
     3661 ttgattacgg cgcgtgcgac cacaagccgg tccatttcgt ggtgatgtat tacgtgcccg
     3721 attcgcagaa gcacgtttat ctgaaggaga tttcagggct cgccaaggcc atccaaagca
     3781 aacaaggcga caaggatttt tcaaaaatac aaacactggc gcaggcgcgc gaatggattt
     3841 tagaccttgt ttcgaccgcg gttgaaacgc agatgaccga cgtcaaagca cggatgatcc
     3901 atctcgaggc gaaacaggcc gcagttacgt ccgcgcccgg agcgggtggt ttgacgcttt
     3961 cgaatctgcc gcacgtggtg gttccacttt cgatactggt ggtgccggga gtgagggcga
     4021 tggtgctttc gcaggacccg gacgtgatcg caaaactgga atcgggcggg gaaattgata
     4081 cgggagcgct ggccaaacaa atgccgtttg accatgcggg ctacacagtg cttttccggg
     4141 gagtcacaaa ttaccaggcc aaacggcaac tctacgattg cctcgccatc aagctcaatg
     4201 gcgcgccgcc ttccgccgac aagccaaagg tttgagtttt tccaggcatt tgaacgcagg
     4261 cattcttttc gcctcgcgca tagggtcatt ttgtacctcg acaaaaccgg cttttgagaa
     4321 cagctttcgc ataggtgacc gtcaacacaa cagcggcagg gattttatga aatttacact
     4381 tgtggctttc cttgggttct ttctggttgc ggaacaaac
//
LOCUS       DASZMM010000472         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4050627, whole genome shotgun sequence.
ACCESSION   DASZMM010000472 DASZMM010000000
VERSION     DASZMM010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4050627"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VG754_11315"
     CDS             complement(<1..541)
                     /locus_tag="VG754_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWF19851.1"
                     /translation="MMPPEVVVFDLGKVLVDFDYSRAARAIAARGTTSAETINQLINQ
                     SSLLVRYELGQMTDEAFFKEVCSVSGFKGDFEEFGKLFGNIFSEMPPMIELHAMLQKK
                     KVPTYIFSNTNGLAIAHIRRTFPFFANFDDYIFSYEHGVMKPDAGLYEVVERKSNRYG
                     EQILYIDDRPENVAAGAARG"
     gene            complement(528..890)
                     /locus_tag="VG754_11320"
     CDS             complement(528..890)
                     /locus_tag="VG754_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19852.1"
                     /translation="MSTDNVANTSLPHRLIAKIKPAPTTQTWQFRGFPPDLTGNEETR
                     EKMPLPCLLVIEEKPDGVFLFRYKLDGQCVGDTWHESVKEAQEQASFEYQLLSSEWKS
                     IPPEIDDVVSFGLKYDAT"
     gene            complement(890..1489)
                     /gene="recR"
                     /locus_tag="VG754_11325"
     CDS             complement(890..1489)
                     /gene="recR"
                     /locus_tag="VG754_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWF19853.1"
                     /translation="MASLPEPLTALTAALARLPGIGPRSAERLALHLVQSDAAEVKQL
                     AQTLLSARERIRTCSVCGSLTEQDPCAICNDSRRDAAMLCLVERAVDIITIEKSGTFR
                     GKYHVLGGKISPLNGVGPEDLRIAELEKRLGKEPIQEIVIALGTDVEGDATSNYLAKR
                     LAKPGLRVTRIAHGLPAGSGLEFADELTLTHALAGRREM"
     gene            complement(1781..2092)
                     /locus_tag="VG754_11330"
     CDS             complement(1781..2092)
                     /locus_tag="VG754_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWF19854.1"
                     /translation="MSSIGKLMKQAARMQQQMEEIQTKLASQTVEATSGGGAVKVVAK
                     CDGTLSSIKIDPQALNPSDAQLVEDMVLTAVNQALAQAKEISNAEMGKVTSGFSLPGI
                     M"
     gene            complement(2257..>2750)
                     /locus_tag="VG754_11335"
     CDS             complement(2257..>2750)
                     /locus_tag="VG754_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWF19855.1"
                     /translation="APGTTDLLELWHRLVEAVGRASPFVRTYLLEAHPVSLTKHLLTI
                     GFDPEFEDHISLVDHARNHTLLQTKLSELGHPNMQIKFVKAERPENWAKPAPTTVAPE
                     PAKVATAPSPAVPAKMATAAAAPPMKEKPVSVAFDKNDFKNDPLIQKALEVFKGQIVD
                     VRA"
BASE COUNT          611 a          783 c          694 g          662 t
ORIGIN      
        1 agccgcgcgc ggctcccgca gccacatttt ccggacgatc atcaatgtaa agaatctgtt
       61 cgccgtaccg gttcgatttg cgctcgacca cttcatacaa tcctgcgtcc ggcttcatca
      121 cgccgtgttc gtatgaaaaa atgtagtcgt cgaaattcgc gaaaaacgga aacgtcctcc
      181 ggatgtgcgc gatggcaagg ccgttggtat ttgaaaaaat ataagtcggc accttctttt
      241 tttgcagcat ggcgtgcaac tcgatcatcg gcggcatctc ggaaaaaata ttaccaaata
      301 gctttccaaa ttcctcgaaa tcacctttga aaccgctgac ggaacagact tccttgaaga
      361 acgcctcgtc agtcatctgg ccaagttcat accgtaccag cagggaggat tggttgatga
      421 gctggttgat ggtttcagcg cttgtcgtgc cacgcgccgc aatggcccgc gcggcgcgcg
      481 aataatcgaa atccaccagc accttgccca gatcaaaaac aacaacttca ggtggcatca
      541 tatttcagac cgaatgaaac tacatcatca atctcgggcg gaatagattt ccattccgat
      601 gacagcagtt ggtattcaaa cgaggcttgt tcttgtgctt ctttcaccga ttcatgccat
      661 gtatcgccaa cacattggcc atctagctta tagcgaaaaa gaaatactcc atctggcttc
      721 tcttcgatta caagtaaaca cggtaaaggc attttttcac gtgtttcttc attcccggtc
      781 aaatcaggag gaaacccgcg aaattgccat gtttgcgttg tcggcgcggg ttttattttc
      841 gcgatcagtc gatgaggcaa actcgtgttg gcaacgttat cagtgctcat cacatctccc
      901 tccggccagc cagcgcatgc gtcagcgtca gttcatccgc aaattccagc ccgcttccgg
      961 cgggtaatcc atgtgcgatg cgcgtcacgc gcaatcccgg cttcgccagc cgtttcgcaa
     1021 ggtaattgct ggtcgcatcg ccctcgacgt ccgtgccgag cgcgatgaca atttcctgga
     1081 ttggttcttt gcccaaacgc ttctccaatt ccgcgatccg caaatcctcc ggcccaactc
     1141 cgttcaacgg cgaaatcttt ccgccgagca cgtgatattt cccgcgaaat gttcccgatt
     1201 tttcgatggt gatgatgtcc accgcgcgtt cgaccaggca aagcatcgcg gcatcgcgcc
     1261 gggaatcgtt gcaaatcgcg caagggtcct gttcggtcaa cgaaccgcaa acggagcagg
     1321 tgcggatgcg ttcgcgggcg gacaaaaggg tttgcgccag ttgtttcact tccgcggcat
     1381 cgctttggac aaggtgcaaa gccaatcgtt ccgccgaacg cggcccgatg cccggcagcc
     1441 tggcgagcgc ggcggtcaat gcggtcagcg gttcaggaag agaagccatt attgattgcg
     1501 agcgaatggt gtgaagaatg ccaaacgctg ccgtcgagta cggttgaaat ggggagcgca
     1561 cgcctctggc gtgccgtgtt tggcgtcccg ccgaacactt tgtgaactga ttttgtcccc
     1621 cacttctcgg tctcgataaa tcaggaattg aagtcctcgc cgagacggct cggacagcac
     1681 gcgagacgcg tgcgctcccc aaaatcccat ctcggttcca atcctttcca atagcaagaa
     1741 catgttttct acaaagcaag ccggaacccg gaacccggaa ctacattatt cccggcaggc
     1801 tgaagccgga agtcaccttg cccatttccg cattggagat ttccttcgcc tgcgcaagcg
     1861 cctggttgac ggcggtgagc accatgtcct cgaccagttg cgcgtccgaa ggattcagcg
     1921 cctgcgggtc aatcttgatg gacgaaagcg ttccgtcgca tttggcaacg actttcaccg
     1981 cgccgccgcc gctggtggct tccacggttt ggctcgccaa cttcgtttgg atttcctcca
     2041 tctgctgctg catacgcgca gcctgtttca tcaatttgcc aatgctcgac atagttcaaa
     2101 tgtgttcagt tgtttctatt ccagtaaacc atctttgccg ggatgcaagc ggtaaatggc
     2161 gggattgtta aatggttaca acgttgcatc gttacatgga cagactccgt catccgatcc
     2221 ttgtaacaat gcaatgattt aaccatgtaa ccaatatcat gcccgcacat ccacaatctg
     2281 ccccttgaaa acttccagcg ccttctggat gagcggatcg ttcttgaaat cgtttttgtc
     2341 gaatgcgacc gataccggtt tttccttcat gggcggagcg gcagcagcag tggccatctt
     2401 tgccggaact gctggcgaag gggcagtcgc caccttggcg ggttccggcg caacggttgt
     2461 gggtgcaggt ttggcccaat tctccgggcg ttccgccttg acgaacttga tctgcatgtt
     2521 cggatgcccc aactcgctca acttcgtctg caacaacgtg tgattgcgcg catggtcaac
     2581 gaggctgatg tggtcttcaa attccgggtc gaacccgatg gtcaacaaat gtttcgtcaa
     2641 cgaaaccgga tgcgcttcca gcaaatacgt ccgcacaaat ggactggcgc ggcccactgc
     2701 ttcaaccaaa cgatgccata attccaacaa atccgttgtt cccggcgcgg
//
LOCUS       DASZMM010000473         5781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3504186, whole genome shotgun sequence.
ACCESSION   DASZMM010000473 DASZMM010000000
VERSION     DASZMM010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504186"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VG754_11340"
     CDS             <1..1173
                     /locus_tag="VG754_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWF19856.1"
                     /translation="IRRYSRLKICAALLLFSCFSAFAETNEIDSALSTTQFRGESSCS
                     SSGCHGGAGEKHDQCIRWSKFDVHTRSYATLTLARSARIADVLKLGDPVQSARCTVCH
                     APFQTIATNDALRKSLDPTAGVSCETCHGPAKKWLLSHTREDYTHQDRVNAGMRDLNN
                     LYVRANTCVACHQNVDADILQAGHPELIFELDGQAVAEPKHWREKPGWSGSQTWFVGQ
                     AVALREMIWQLSTEKAPSQADLEREQGLLWVVESVGWNSNVPQLMAAPSLDFKSDKSY
                     QQRVQSWADQMANSATYFPCPPDATPHSLGLLAATAGDFRNAKVSHAIQARRAERLVL
                     ALDRLMAGLNDDALAKGLDAPLNKLFKDAQSLPDFDSKKFADDLDAFRKALESTPK"
     gene            1257..1655
                     /locus_tag="VG754_11345"
     CDS             1257..1655
                     /locus_tag="VG754_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF19857.1"
                     /translation="MKTAKKLLHTRYRVNDLEQTAKFYREVLGLEEVRRHKSSRGSEL
                     VFMKAPESEETIELCYFPGSGSVQVQPDLTHLAFEVDSLEEFGKHIAALGYSYSDGPH
                     LRPDGGGIAFVDAPEGYEIELIQRPKTMEL"
     gene            1806..2645
                     /gene="folP"
                     /locus_tag="VG754_11350"
     CDS             1806..2645
                     /gene="folP"
                     /locus_tag="VG754_11350"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWF19858.1"
                     /translation="MLFHARQFEFRFPRPRLVMGIVNVTPDSFSDGGKYFDHAAAIAH
                     AEELVREGADILDVGGESTRPNAAPVSEAEELERVIPVIKTLAQRVKIPISIDTMKPA
                     VAQAALEAGASIVNDVAANRADDTMWKLVAESGAGYIVMHMQGTPQTMQINPAYQDVV
                     REVKEFFGERLGRLKAAGVALEQVMLDVGIGFGKTAEHNLQLLAALQSFTSLERPMLL
                     GISRKSFIGKIAGGGNERLPGALACTVLAAAAGVQMFRAHDVAATVQALRMTEAILAR
                     RDR"
     gene            2642..3493
                     /gene="cdaA"
                     /locus_tag="VG754_11355"
     CDS             2642..3493
                     /gene="cdaA"
                     /locus_tag="VG754_11355"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HWF19859.1"
                     /translation="MNLFLTQNDWWRIALEIGLLAVGIYYVLMFVRGTRGWSVAIGFL
                     LLMAMTLATWALKLEVVTWLLEKFFPFSAFAILVIFQPELRRMLSELGSLSMFYTARE
                     QRENIEVIIQSVERLSEVHIGALIAVEQAIQLQDVVESGVVVDCEATPEMIETIFFPN
                     NAIHDGGIIMRGDRIAYAACIFPLTQRQDLNKSLGTRHRAAIGLTEETDAVVVAVSEE
                     TGLISYAYKGQLVRGVSQEELRAFLTSVLLKGSQPHGLVQASRKWLGTRELKHPPAVQ
                     KATHKSP"
     gene            3496..3918
                     /locus_tag="VG754_11360"
     CDS             3496..3918
                     /locus_tag="VG754_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HWF19860.1"
                     /translation="MALRDIVLHNFWWKLLSLLLAALTWLTIETALRREEMLRQTPVV
                     GSYSRDFPAVPITLLTASTNANHFRIDPDVASVRISGPADQLQKLQERDIHVYIDLSD
                     VGDEKQLRRNIQAQVPKDLKVEDLKPTYTSVERISNAK"
     gene            3935..5317
                     /gene="glmM"
                     /locus_tag="VG754_11365"
     CDS             3935..5317
                     /gene="glmM"
                     /locus_tag="VG754_11365"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWF19861.1"
                     /translation="MTAQKKIFGTDGVRGTANIEPVTAETALKLGRAAGHVFKNLETH
                     SRGRGKFKIVIGKDTRLSGYMLENAISSGVLSMGVDVLFIGPLPTPGVAYVTRSLRAD
                     AGIVITASHNPYDDNGIKFFRADGYKLDDQIEAKIEGLVFSGEIESIRPTAGAIGKAV
                     RIDDALGRYIEYAKTSFPRGMTLEGVRVVVDCAHGAAYKSTPCVLRELGAEVIVYGNH
                     PDGMNINKECGSMHPEQMCQKVFEHRADIGIAHDGDADRVLLCDETGTLIDGDDIMAI
                     AGRDMLEVGQLAEKTLVSTVMSNNGLDMFIKQHGGNVVRTAVGDKNVIDEMLRNGSNF
                     GGEQSGHLIFRDYSTTGDGLVCALQMLRIMKTSDKPLSKLARCWTRFPQLVTNVKVRE
                     KKPFEQLDGVMKLVTQAEDAVKAEGGRVLLRYSGTEPKARLLMEGRDLGALEKHSKLI
                     CEAIKKHVGA"
     gene            5522..>5781
                     /locus_tag="VG754_11370"
     CDS             5522..>5781
                     /locus_tag="VG754_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19862.1"
                     /translation="MKSLGLICVLSLALAQVTFGEAASEGAKSPQPPPPLVPGAKVVP
                     LWPAGSPTLKNFDEQEAVTYAKGSTTSIVRVTNINNPSIELHL"
BASE COUNT         1305 a         1555 c         1678 g         1243 t
ORIGIN      
        1 attcggcgat acagcagatt aaaaatctgc gctgcgctgc tgctgttcag ttgtttttcc
       61 gcctttgccg agaccaacga aatagattca gccctttcca caacgcaatt tcgtggtgaa
      121 tcatcgtgca gttcctccgg atgccatggc ggcgcgggcg agaaacatga ccaatgcatc
      181 cgctggtcga agtttgatgt tcatacccgt tcctacgcga ccctaacgct ggcccgctcg
      241 gcgcgcatcg cggatgtttt gaagcttggt gacccggtgc agagcgcgcg ttgcacggtt
      301 tgccatgcgc catttcaaac gattgcaacc aatgatgctc tccgaaaaag tctcgacccc
      361 accgcgggag tttcctgcga aacctgccat gggcctgcca aaaaatggct gctttcccac
      421 acacgcgagg attacaccca ccaagaccgc gtgaatgccg ggatgcgcga tttgaacaat
      481 ctttacgtgc gcgcgaacac ctgcgtcgcc tgccatcaaa atgttgacgc ggacattctc
      541 caagccggcc atccagaatt gatatttgaa ttggacggcc aggcggttgc cgaaccgaaa
      601 cattggcgcg agaaaccggg ctggtccgga tcgcaaacgt ggttcgtcgg tcaggccgtt
      661 gctctgcgtg agatgatctg gcaattgagc actgaaaaag cgccctccca agctgacctt
      721 gagcgggagc aagggttgct ttgggtagtg gaaagtgttg gttggaattc gaatgtcccg
      781 caacttatgg cggcaccatc gttggatttc aagtcggaca aaagttatca gcagcgggtg
      841 caatcgtggg ctgaccagat ggcgaattca gccacctatt ttccttgccc gccggacgcg
      901 actcctcaca gcctcgggct tttagctgcg accgccgggg attttcgcaa tgcgaaagtc
      961 tcccacgcca tccaagcccg ccgcgctgaa cgcctcgtcc tcgctcttga ccgtttgatg
     1021 gctggattaa acgacgatgc ccttgccaaa ggcctcgatg ctccgctcaa taaactcttc
     1081 aaagacgcgc aatcattgcc ggattttgac tcgaaaaaat tcgctgatga tcttgatgcg
     1141 tttcgaaaag cgctcgaatc aaccccaaaa taatctttct ttccgtccct caactctcag
     1201 ccattaacct tcaaccattt cgttgctttg gtttaaagcc gggttatgct tcggccatga
     1261 aaacggccaa aaaactgttg catacccgct accgggtgaa tgacttggaa cagacagcga
     1321 aattctatcg cgaggtgctg ggtttggagg aagtgcggcg tcataaatca tcgcgtggct
     1381 cggaattggt attcatgaaa gcgcccgaaa gcgaggaaac catcgagctt tgttattttc
     1441 cgggcagcgg gtcggtgcag gtgcagccgg atttgaccca tcttgccttc gaggtggaca
     1501 gcctggaaga attcggcaaa cacatcgccg cgctgggcta cagttattcc gatggcccgc
     1561 atctccgtcc cgacggcggc ggcatcgcct tcgtggatgc gcccgaggga tacgagattg
     1621 aattgattca acggcccaag acgatggagc tgtgagcgag ttccaagttt aaggtttaaa
     1681 gttcaaagtt gaagaagttt cggttgacct tcgccatgtc gcatgattga ggctccagtc
     1741 acgtgtaaga ttcagtcgcg acccgatttt aaactttgaa cccggaactt tgaacttgaa
     1801 aacgaatgct cttccacgcc cgccaatttg aattccggtt tccgcgtccg cggctggtga
     1861 tgggcatcgt gaatgtcacg ccggattcgt tttcggacgg cggaaagtat ttcgaccatg
     1921 cggctgcaat cgcccacgcg gaagaattag tgcgcgaagg cgcggacatc cttgacgtgg
     1981 gcggcgaatc cacgcgtcca aacgccgcgc cggtaagtga agcggaagaa ttggagcgag
     2041 tcattccggt catcaaaacg ctggcgcaac gggtgaagat tccgatctcg attgatacga
     2101 tgaaaccggc ggtggcgcag gcggcattgg aagcgggcgc gagcatcgtc aatgacgtgg
     2161 cggcgaatcg cgcggacgat acgatgtgga agctggtcgc cgaaagcggc gcgggttaca
     2221 ttgtcatgca catgcaggga acgccgcaaa caatgcagat caacccggct taccaggatg
     2281 ttgtgcgcga ggtaaaagag ttttttggcg aacgattggg gcggttaaag gcggctggag
     2341 tggcgttgga acaggttatg cttgatgtgg gaatcggttt tggaaagaca gccgagcata
     2401 atttgcagtt gctcgcagcg ctgcagagtt ttacaagttt agagaggcca atgttgctgg
     2461 gtatctcgcg caagtcgttt atcggcaaga ttgccggtgg cgggaacgag cgattgcccg
     2521 gggcgctggc ttgcaccgtg ctcgctgcgg cggcgggagt gcagatgttc cgggcccacg
     2581 atgtggccgc gaccgtgcag gcattgcgga tgacagaggc aatattggcc cgccgagatc
     2641 gatgaattta ttcctgacac agaatgattg gtggcgcatc gcgctggaga ttggtctcct
     2701 ggcggtcggc atctattatg tgctgatgtt cgtgcgcggc acgcgcggct ggtcggtggc
     2761 cattggtttc ctgctgctga tggcgatgac gctggcgact tgggcgttga aactggaagt
     2821 agtcacatgg ttgctggaaa aattcttccc gttctcggcg ttcgcaatct tggtgatttt
     2881 ccagccggaa ctgcggcgaa tgttgtccga gttgggaagt ctttcgatgt tttacactgc
     2941 gcgtgaacaa cgcgagaaca tcgaagtcat catccaatcg gttgaacggc tttcagaagt
     3001 ccacatcggc gcattaatcg ccgttgagca ggccatccaa cttcaggacg tggtggaatc
     3061 gggcgtggtg gtggattgtg aggcgacgcc ggagatgatc gagacgattt tctttccgaa
     3121 caatgccatt cacgacggcg gcatcatcat gcgcggcgac cgcatcgctt atgcggcatg
     3181 catctttccg ctgacacaac ggcaggactt gaacaaatcg ctcggcacgc ggcatcgcgc
     3241 ggccatcggg ttgaccgagg aaaccgacgc ggtggtcgtc gctgtttccg aggaaacggg
     3301 tttgatctcg tacgcgtata aaggccagtt ggtgcgcggg gtgagccagg aagaattgcg
     3361 cgcgtttctg acctcggtgc tgctgaaggg cagccagccg cacggcctgg tgcaggcgtc
     3421 gcgcaaatgg ctcggcacgc gcgaactcaa acatccgcct gccgtgcaaa aggcgaccca
     3481 taaatcaccc tgaccatggc cctgcgcgac atcgtgctgc acaatttctg gtggaagctg
     3541 ctctccctgc tgctggcggc gttgacgtgg cttaccatcg aaactgcgtt gcgtcgcgaa
     3601 gaaatgctcc ggcaaacgcc ggtggtcggc agctattcgc gcgattttcc cgcagtgccc
     3661 atcacgctgc tcactgcttc cacgaatgcg aaccatttcc gcattgatcc cgacgtggcg
     3721 tccgtgcgca tcagcggccc ggcggatcaa ttgcaaaaat tgcaggagcg cgatatacat
     3781 gtctatattg acctcagcga tgtcggcgac gaaaagcaat tgcgtcgcaa tatccaggcg
     3841 caggtcccga aagatttgaa agtcgaagat ctcaagccga cttacacaag cgttgaacgc
     3901 atctcgaacg cgaaatagaa caaccattcc caccatgaca gcccaaaaga agatattcgg
     3961 caccgacggt gtgcgcggca ccgcgaacat cgagcctgta acagccgaaa ccgcccttaa
     4021 actcggccgc gccgccggcc acgttttcaa gaacctcgaa acacactcgc gcggacgcgg
     4081 caagttcaag atcgtcatcg gcaaggacac gcgcctttcc ggttacatgc tcgaaaacgc
     4141 aatttcatcc ggcgttttgt ccatgggcgt ggatgtgttg ttcatcgggc cgcttcccac
     4201 gccgggcgtt gcgtatgtga cgcgcagttt gcgcgcggac gcgggcatcg tcatcaccgc
     4261 atcgcacaat ccttacgacg acaacggaat caaattcttt cgcgctgacg gttataaatt
     4321 ggacgaccag atcgaggcga agattgaagg actcgttttc agcggcgaga ttgaaagcat
     4381 ccgtcccacc gcaggtgcga tcggcaaggc ggtgcgcatt gatgacgcgt taggccgtta
     4441 catcgaatac gccaagacct cattcccgcg cggcatgacg ctggagggtg tgcgcgtggt
     4501 ggtggattgc gcgcacggcg ccgcttacaa atccacgccg tgcgtcctgc gcgaactcgg
     4561 cgcggaagtc atcgtttatg gcaaccatcc cgacggcatg aacatcaaca aggaatgcgg
     4621 ctcgatgcat cccgaacaga tgtgccagaa agttttcgaa catcgcgcag acatcggcat
     4681 cgcgcatgac ggtgacgcgg accgcgtctt gttgtgcgac gaaaccggga cgttgattga
     4741 cggcgacgac atcatggcga tcgccggacg cgacatgctc gaagtcggcc aactcgcgga
     4801 aaaaacgctc gtctcgaccg tgatgagcaa caacggcctg gatatgttca tcaagcagca
     4861 tggcggaaac gtggtgcgaa cggcggtcgg cgacaagaac gtgattgacg agatgttgcg
     4921 caacggctcg aacttcggcg gcgagcaaag tggtcatttg attttccgcg attacagcac
     4981 gacgggcgac ggattggttt gcgcgttgca gatgttgcgc atcatgaaga catccgacaa
     5041 gccgctatca aaactcgcgc gctgctggac gcgctttccg caactcgtca ccaacgtgaa
     5101 ggtgcgcgag aaaaaaccgt tcgagcaatt ggatggcgtg atgaaactcg tcacacaagc
     5161 cgaggacgcg gtgaaagccg aaggcggtcg cgttctgctg cgctattccg gcactgaacc
     5221 caaagcgcgt ttgctcatgg aaggccgcga tttgggtgcg ctcgaaaaac atagcaagct
     5281 catctgcgaa gccatcaaga agcatgtcgg agcgtgaagc gtgtttggtt aaaagattga
     5341 atgggttaca tcgttccaag acgccgagac aatctgacgc tggtgggcga gcgtaatcgc
     5401 aagccttcaa tgactcgcac aaaatcttcc gcgacgacgt gcaatgtttc ttctttgatc
     5461 gcacaaaaat ttgctccgga aaaaattggc aatgtagtgc ttctgcggta agcttcgccg
     5521 catgaaatcc cttggcctca tctgtgttct ttcattggcc ctcgcgcaag tcacctttgg
     5581 cgaagcagcg tcagagggcg cgaagtcgcc acaaccgcct ccgccgttgg ttccaggagc
     5641 caaggtcgtg ccgctctggc ccgctggttc gccgacattg aaaaatttcg acgaacagga
     5701 agcggtcact tacgcgaagg gcagcaccac cagtattgtt cgggtgacaa acataaacaa
     5761 tccttcaatt gaattgcatc t
//
LOCUS       DASZMM010000474         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2950923, whole genome shotgun sequence.
ACCESSION   DASZMM010000474 DASZMM010000000
VERSION     DASZMM010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2950923"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VG754_11375"
     CDS             complement(<1..746)
                     /locus_tag="VG754_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HWF19863.1"
                     /translation="MTASLGAALLAFPITMLAATKDEGVQYNRDIRPILSENCFPCHG
                     PDSAARKASLRLDHFDDAILPRKDSSPAIVPGKPDASEVIHRITATNSDDIMPPVKTT
                     KKLTAHERELIAKWISSGAKYQPLWSFIPPARPEMPKVHNQRWVRNPIDAFILARLEK
                     EHLKPAAEANRRTLIRRVTLDLIGLPPTPEEVAAFVKDKSPNAYEKVVDRLLASPHYG
                     EHRARYWLDAARYADSNGIHFDNYREMWAYR"
     gene            complement(914..2017)
                     /gene="ald"
                     /locus_tag="VG754_11380"
     CDS             complement(914..2017)
                     /gene="ald"
                     /locus_tag="VG754_11380"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWF19864.1"
                     /translation="MIIGVPKEIKDHEYRVALLPSAAYQLVKRGHQVLVERGAGAGAG
                     YPDLDYEQAGATMVSDHRAIFEKAGMIVKVKEPLPPEYPLLRQDQILFTYLHLAASRS
                     LTEALLKSGVTGIAYETIEVNRRLPLLEPMSEIAGRMSVLVGGYFLAKHHGGSGVLLG
                     GVPGVLPGRVVVLGGGASGINAARMATGLGADVTILEVDLERMRFLDITLHTAHTLYS
                     SEAHLMGLLPTVDLLIGAVLVPGAKAPKLINREMVRQMRPGSVLVDIAIDQGGCAETS
                     RATTHQNPTYVDEGVTHYCVANMPGAYARTATQALTNVTYRYVEMLADLGLAEACQKQ
                     PALIGGVNVMDGKVTHKAVAEAHGLPCLAPVMK"
     gene            complement(2208..3797)
                     /locus_tag="VG754_11385"
     CDS             complement(2208..3797)
                     /locus_tag="VG754_11385"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HWF19865.1"
                     /translation="MKKWLAKTKRAVGEIRDLLDEKTFHAPELRPLSRVHRFIHFWVL
                     VWKSFSRNRGPARAAALAYTTLLALIPMIFVVVSVSSSILRQEGGQRVNKFIDHFLVS
                     MTPPLAVTNNPQNEVNSTSTNTQSSANPTNTENFAANSQTNFPSATVGSGNTNAPSDA
                     NTAIASRQQLVQKINDFIRSIQSGTLGVTGIAGLIIAAILMLRSIETTFNDIWGVTQS
                     RHWWAQVMQYSAVLLIGPTLLAVGLGLTGSQHLESAREMFTRMPFFSHLLFKTTPVVL
                     LCLAFAGFYVVMPNTKVHWSAALVGGLVSGLLWHLNNYLSVLYVSRWITNSKIYGSLA
                     VIPIFMAGVYISWWILLFGAQVAYAYQNRAAYLQEKQTEVVNQRGREFVALRLMECIG
                     MRFQHAEPPASIAEIAERLAVPSRLIQQIIQNLLSSRLVVEISGLELAYVPARPLEQI
                     TCHDILLAMRVGWGQELATREEPARVGVFGEFEKIMEAEKRAASSVTLRMMVDRTEHL
                     AAMAGKPVKAVTEGKGSGQVE"
BASE COUNT          895 a         1100 c         1117 g          869 t
ORIGIN      
        1 cgatatgccc acatctcgcg gtaattgtcg aagtgaatgc cgttcgaatc cgcgtatcga
       61 gctgcgtcga gccaatagcg tgcgcgatgt tctccataat gcggcgatgc cagcaggcga
      121 tccaccactt tttcgtacgc gttgggcgat ttatctttga caaaagcggc gacttcctcc
      181 ggggttggcg gcagaccaat caaatcaagt gtcacccgcc ggatcaacgt gcgacggttt
      241 gcttccgccg caggtttcaa atgctccttt tccaatcgcg cgagaatgaa agcatcaatc
      301 ggattgcgca cccaacgctg gttatgcacc ttgggcattt ccgggcgagc aggcggtata
      361 aatgaccaaa gcggctgata ctttgcgccg ctcgaaatcc attttgcgat caactccctt
      421 tcgtgcgccg tcagtttctt tgttgtcttg accggcggca tgatgtcatc agaattcgtc
      481 gcggtgatgc gatggatgac ttcgctggcg tccggtttgc cggggacaat cgccggagac
      541 gaatctttgc gcggcaggat ggcgtcgtca aaatggtcca gcctcagcga tgctttgcgc
      601 gcggcggaat ccggtccgtg acaggggaaa caattttcgg aaaggatggg ccggatgtcg
      661 cgattgtatt ggactccttc atcctttgtc gcggccagca tagtaatcgg aaacgcgagc
      721 aacgccgcgc ccaaacttgc cgtcaaaaga gaaagaacat cctttagccg acgcgcgtac
      781 aacgcacgca accaactgcg actattggag tcgagggtca tcgccgataa ttataacatt
      841 gtcgcccagc ctattgcaat aattgacggc gcgcaaaggc tggttattca acgcaactgt
      901 atggggtgtc atcctacttc atgactggcg cgaggcacgg caacccatga gcttcggcaa
      961 cggctttgtg tgtgaccttg ccgtccatga cgttgacgcc gccaatcaag gcaggttgtt
     1021 tctggcaggc ttcggccaag ccgaggtcgg cgagcatttc gacataacgg taagtgacat
     1081 tggtcaacgc ctgcgtggct gtgcgggcat aagcgcccgg catgttggcc acgcaatagt
     1141 gagtcacgcc ttcatcgaca taggtcgggt tttgatgcgt ggtggcgcgt gaagtctcgg
     1201 cgcaaccgcc ctggtcaatg gcgatgtcca ccaacacgct gcccggacgc atttggcgaa
     1261 ccatttcgcg gttgatcaat ttcggggctt tcgccccggg cacgagcacc gcgccgatga
     1321 gcagatcaac cgtcggcaac agacccatca aatgcgcttc actggaataa agcgtgtgag
     1381 cagtgtgcaa tgtgatgtcg agaaaccgca tccgttccaa atccacttca aggatggtca
     1441 cgtccgcgcc aagcccagtc gccatgcgcg cggcgttgat gccggaagcg cctccgccga
     1501 gaacaacgac acggcccggc aacacgcccg gcacgccgcc aagcaacacg ccgctgccgc
     1561 catgatgttt cgccagaaaa tatccgccga ccaaaaccga catgcgcccg gcgatttcgc
     1621 tcatcggttc cagcaatggc aatcgccggt tgacctcaat cgtttcgtac gcgattcctg
     1681 tgacaccgga tttgagcagc gcttcggtca acgagcggct tgcggcaaga tgcagataag
     1741 tgaaaagaat ctgatcctgc cgcagcaaag gatattccgg tggcaacggc tccttcactt
     1801 tcacgatcat tccggctttt tcaaaaatcg cgcgatggtc ggaaaccatc gttgcgccag
     1861 cttgttcgta atcgaggtcg ggatagcccg cgccagcgcc tgcgccccgt tcgacgagca
     1921 cttggtggcc gcgtttgacg agttgatacg ccgcggaagg cagcaacgcc acccgatact
     1981 catggtcttt aatttctttt ggaacgccga taatcataaa tcgcccgaga gactaacgaa
     2041 aggaagcacg cacttcaata ggtgtgttaa acatgggggt gagaaacgga gtggtggagt
     2101 aatgggaaag agtagaggta tgcgcccatc ggagaaaaca catccgaaag cgcggtcaaa
     2161 gtcttttgcg attccgcttt tcaacattcc aatactccaa tgctccatca ctccacttga
     2221 ccgcttcctt tcccttccgt caccgccttg accggtttgc cggccattgc ggcgagatgt
     2281 tccgtacggt ccaccatcat gcgaagtgtc acggatgacg ctgcgcgctt ctcggcttcc
     2341 atgatttttt caaattcgcc gaacacgccg acgcgcgccg gttcttcgcg cgtggcaagt
     2401 tcctggcccc aacccacccg catcgcgagc aggatgtcgt gacaggtgat ttgttccagg
     2461 ggccgcgcgg gaacataagc gagttccagc ccggaaattt cgaccacgag acgcgatgac
     2521 aaaaggttct gaatgatttg ctggatgagg cgcgaaggca ccgctagccg ctcggcaatc
     2581 tccgcgatgc tcgcgggcgg ttcggcatgt tgaaagcgca ttccgatgca ttccatcaag
     2641 cgcagggcga cgaattcccg gccgcgctgg ttgacgactt cggtttgttt ctcctgcaga
     2701 tatgcggcgc ggttctgata cgcataagcc acctgcgcgc cgaagagcaa tatccaccat
     2761 gaaatataaa cgcccgccat gaagatgggg atcaccgcga gactgccgta aatcttgctg
     2821 ttcgtgatcc agcgcgaaac gtaaagcacg ctcaggtaat tgttgagatg ccagagcaaa
     2881 ccgctgacca atcctcccac cagcgcggcg ctccaatgca ctttcgtgtt gggcatgacc
     2941 acgtaaaacc cggcaaatgc gaggcacaac agcaccactg gcgttgtttt gaacagcaga
     3001 tggctgaaga atggcatgcg cgtgaacatt tccctggctg attccagatg ctggctgccg
     3061 gtcaatccca gaccgaccgc gagcaacgtg ggcccgatca acagcaccgc tgaatattgc
     3121 atgacttgcg cccaccaatg ccggctctgc gtcacgcccc agatgtcgtt gaatgtggtt
     3181 tcaatgctgc gaagcatcaa aatggccgcg atgatgagtc ccgcaatgcc ggtaacgccc
     3241 aacgtgccgc tctggatgct gcgaatgaaa tcattgatct tctgcaccaa ttgctgacgg
     3301 ctcgcaatgg cagtgttcgc atccgatgga gcattggtgt tgcctgaacc aacagtcgct
     3361 gaaggaaagt tcgtttggga atttgcggca aaattttcag tgttggtggg attggccgag
     3421 gattgggtgt tcgtgctggt tgaatttact tcgttttgag gattgttggt cactgcaagc
     3481 ggaggcgtca tgctcaccaa aaaatggtcg atgaatttgt tgacgcgctg gccgccttcc
     3541 tgccggagga tgctgctcga aacgctcacc accacgaaaa tcatcgggat gagcgcaagc
     3601 aatgtcgtgt aagccagcgc cgcggcgcgc gcgggaccgc gattccgcga aaagcttttc
     3661 cagaccagca cccagaaatg gatgaatctg tggacgcgcg acaatggcct gagttcgggc
     3721 gcatggaacg tcttttcatc cagcaaatcc cggatctcgc cgacggcgcg tttggtttta
     3781 gccagccact tcttcacgtt gtctccgagg aacgctaaca gagcaaggtt tcaagtcaat
     3841 cgaccggaaa tggagtggtg gagtaaagga gttttgggga gagatccatg gcttgactgc
     3901 gcataaaaga atggcttgac tagccttgac tcgttttggg ctgaaaatag agtaatgcat
     3961 tgatgagcaa taggttgttg c
//
LOCUS       DASZMM010000475         8725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5432929, whole genome shotgun sequence.
ACCESSION   DASZMM010000475 DASZMM010000000
VERSION     DASZMM010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5432929"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..5144
                     /locus_tag="VG754_11390"
     CDS             15..5144
                     /locus_tag="VG754_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF19866.1"
                     /translation="MTNGLTITPTGIHNLGTWTRGFIEAPQTGPYTFWIASTDEGQLW
                     LSTNESPANLVELADNPLPITYGSYLPVNQSPKVSLVAGKKYYFEMYQKNGNDAPHAE
                     VGWQLPDGSYEAVISPNHIWPYPVDRTNPSYPAVSVAPTLLTDYSDSNAGTDTQVANM
                     PSSISVLSGQPLDLLNTCEASQPATVSWYSNNVLVANANLLNYHISGIGPGQNGDTYT
                     VVVSNSLGVASNSMTLSVTVDTAPPVLVDALVLGNPAGDISVVFDKGIDPVTGGNAGN
                     YSLNNGATVTSAVIGSDNATVLLRVSGLTVGTSYNLSVNNVNDLSGNTIAANSSVPVE
                     ANLNTAYRFDETSGSVANDFSGNGNNGTLAGGVQPGFTGKVLRSYKFDGASGHVAMPS
                     GYANFQSGLTVAVWARPTSSLVTWARIMDLGNGSSSDNIIFARNGTSPNLTFETRLSP
                     PAGATTGQVTASNALYLSQWQHIAATLDSSGNVTIYRNGVVVQTGVQTSLPNIITRTN
                     CYVARSDWSTDSYYQGEYDDFRLYNRILSSNAILALASGGGPDDSGGVPEVSVVATTP
                     ITAERSTPPGVFTVTRTGSTNGSLAVQYTLSGTATNGVNYTNLSGSVTIPAGASTAQV
                     LIGPINLSFSDLSRTAILTLSGSTNYTMALANSDTVTIQNNDVVPVAYVATADNAKST
                     QPNTTVDVWFGAAVTLPSATNLSNYALSGGLTVTSATLDTSHNLRVILGVSTPVPTNT
                     ILSVSGVLDAAGNGASTNITIRVGLPSCDVVALVFHQGRSTGFTSVTDGVVNNVNNVP
                     TAGRTGFDTYNGAGTVQFGGMIYQSPVDIQAIKVDLGQQFSDGGDWGSQPHVYILKNP
                     KDTDSTQPQVDTNDWVQVPAVLISGSSPFLISGTLPNPSPNTPYVYDLSGLTPAQRSG
                     YGWAVGGVQASGAHAFMSFSELRAYGVASTNYPTVSFALQPVNATVTAGQRAIFSSRL
                     NMSNLMATVLYPPGNDAGVNVPIVGYQWYMDGSAVTGATNATYTTDQTTTAETGNQYY
                     LVASNHFFSITSSTVTLTVNARTSPPVVVAATVDLYANIDVWFDEPVDPGTSQNLGNY
                     ALNDPALTLQSVTQNSYQTRAALTYTGTQSVSNLTLTVSGVADTFGNTLGSQTVPLIP
                     QDWPVQNLVANAYQQGRAAMLTTVTDGQVVIHTAATFAGGSMGQTHFAGLIYNQPQVF
                     GVVKVDLGNQFADGGDWAFQPNVYILKQSIDPNSSRPESTPSVNGAPAWVQVPATLIS
                     GNTYSWEIDGPVNSLEVNGPTAFDLSHLPASQRTGYGWAVGGVVPNALGSAYTGITPA
                     AEFLSLSELRGFGVTPSAVTGAPQVLEDIPSTLAYPEGLPLALSVLVGGTQPISYQWK
                     RNGTNLTDGGRISGSQTSALNIAEVLAADAGTYQLFMTNTSGTASSTASALSITRIAL
                     NNGAGWVQNGAGGTTDTYGPPISGNVLALTDGLTGETRSAWLNAPQYIGAFQASYTYQ
                     DMGGGGADGAAFVMQNSTSGTTALGGGGGALGYNGITPSAALEFNIYSGNTNGYAFRT
                     NGATGIPYNRTGALRVDSGDPIGVSLNYNGTVLTMTLTDAVAQVSFATNLPVNIPAIV
                     GSNSAYVGFTGASGGVASTQKISNFVFASLPQLSIQPASGNNYLFTWPQTAGGFVLLQ
                     NSNLSTTNWTAVNIPLVVTNGQNQVTVPVQPGTEFYRLRLP"
     gene            5441..8086
                     /locus_tag="VG754_11395"
     CDS             5441..8086
                     /locus_tag="VG754_11395"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HWF19867.1"
                     /translation="MKHLTRKFFFRSKLEMLFLAACIGICNSAGAAATEPRGLDAITQ
                     FDRLPYLKPDTMEEGQSSFDRSGGNSDFGNFLYRDPDGTNKVLLDVAGPGTVYRIWFT
                     GFNQATDYIKVYFDGETTPRIDMLLNDLYAGTNAPFLSPLVANNLVSSGGFYCYLPLP
                     FNRSIKIVSNATAGSFYYNIGYHVYSQDTTVTTWTGLEDSTAARNLWKNVGSDPKSDS
                     GNVVVSNAFNLAPSGSQTLLDISGPYSISSIKLNFPGINFAAPLTITDDGRADKGYSQ
                     FQMAINPTNTGVELVRRLDYGIGNQKANVYVDGTLIGQWFDAGSDQTYHWRDSGFNIP
                     STYTTGKSAITVKVSYVSSDVDWNEFYYWAYSLSAASSNLTDSLDVGNSSSETNHAYV
                     INTQTWSGVRTFQYPPGEDPNTIELLTNTWLSISFDNETNPSVYAPVSSFFAMGQFAP
                     YPVRALPVGVDANSNLYCYFPMPFGSHATVQLVSFRAVTTSNITCELRYKPFTDSLAR
                     VGYFKTQYNSELPTTNGADIVMLDAEGTGHFVGVVESMMGVVSRYYLEGDERIYVDNS
                     ASPAIYGTGTEDFYDAGWYFQNGLFSLPINGNPAHVADANYDRTTAYRFFLSDAIPFR
                     KHITVGIQHGGVDDSSENVWTLAYYYCQPTNHAILTDQLLVGNTPSEAAHSYSINTQT
                     WTGTRSYTFDGNFDTVIVTNTGRAHKGYSQFTMALSPTNAGAILRRQFDQGVANQKAN
                     VYVDGVLAGVWYRAGSNTIHNWRDDDFMVPSAFTSGKNAIQVQVQFLSSSNDWNEFVY
                     SIYSLPIITNATPPRLIGGTWRGNAFAISLSGDPHTGYGVWASTNLANWGWLGTASET
                     SPGIYGFTDTTANGIPQRFYRGKSP"
     gene            8083..8295
                     /locus_tag="VG754_11400"
     CDS             8083..8295
                     /locus_tag="VG754_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19868.1"
                     /translation="MKSKLPGPQVAASATRAPAILFLFPTAGGDDTGFSEMTDAQCAR
                     AIALFNWRKNKPTKLRWFEQLPKYGE"
     gene            8472..>8725
                     /locus_tag="VG754_11405"
     CDS             8472..>8725
                     /locus_tag="VG754_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWF19869.1"
                     /translation="MNRGGRNAGPGIGDRKDARHPEPEEEKDHRPEEKSSEEKDDAAK
                     QGPQRPPAYKRPGFLIIVSVVLLVLIVGGLLYWLHARHFVS"
BASE COUNT         1968 a         2464 c         2352 g         1941 t
ORIGIN      
        1 gctattccga gactttgacc aatgggctga caatcacacc gacagggatc cacaatctcg
       61 gcacttggac gcgtggtttc atcgaggctc ctcaaaccgg cccatacaca ttttggattg
      121 cgagcacgga cgaaggtcag ttgtggctca gcacgaatga atctcccgcc aacttggttg
      181 aactggctga caacccgctg cccatcacat acgggtcgta cctgccggtg aaccaatcac
      241 ccaaggtatc attggtcgcg ggcaaaaaat actattttga aatgtatcaa aaaaacggca
      301 atgacgcgcc gcatgctgag gtaggctggc agttgccgga tggcagttat gaagccgtga
      361 tttctccgaa tcacatctgg ccctacccgg ttgaccggac aaatccatcc tatccggctg
      421 tcagtgtggc tccgactctt ttgaccgact actccgattc aaatgcgggc accgataccc
      481 aggtggccaa catgccatcc agcatttcgg tgttgagcgg acaaccgttg gacctcctca
      541 atacttgtga agcgagccaa cctgccacgg tttcttggta tagcaacaac gtgctcgtgg
      601 cgaatgcgaa tctgttgaat taccacatat ccggcatcgg ccccgggcag aatggagaca
      661 cttacaccgt cgtcgtcagc aattcgctcg gcgtggccag caattcgatg acgctttctg
      721 tcacggtgga tacggcacct cctgttcttg tggacgcgct tgttttggga aacccggcag
      781 gggatatttc ggttgttttc gacaaaggga ttgacccggt cactggagga aacgcaggga
      841 attattccct caataacggt gcaactgtga ccagcgcggt gatcggttcc gataatgcca
      901 ccgtgttgtt gcgcgtatca ggcttgacgg ttgggaccag ctataacctt tcagtgaaca
      961 atgtgaatga cctttccggc aatacaatcg cggccaacag cagtgtcccg gttgaagcca
     1021 atttgaacac agcgtaccgc ttcgatgaaa ccagcggcag cgtcgccaat gatttttccg
     1081 gcaacggcaa caacggcacg ttggcgggcg gtgtgcagcc tggattcacc ggcaaggttt
     1141 tgcgatcgta taagtttgac ggcgcatccg gtcacgttgc gatgccaagc ggttatgcca
     1201 acttccagtc gggtttgacg gttgcggtgt gggcgcgacc cacttcgtca ctggtcactt
     1261 gggcgcgcat catggatttg ggcaatggat cgagcagcga taatatcata ttcgcgcgca
     1321 acggcaccag tccgaacctc acttttgaaa cgcgcctgag tcctcctgcg ggtgcgacca
     1381 cgggacaagt gacagccagc aacgcattgt atttgagcca atggcaacat attgcggcga
     1441 ctttggacag ctcaggcaat gtcaccattt atcgaaacgg tgtcgtcgtc caaactggcg
     1501 tgcaaaccag cctgccgaat atcatcactc ggaccaactg ctatgtggcg cgcagcgatt
     1561 ggagcacgga cagctattat caaggcgaat acgatgattt ccgtctttat aatcgtatcc
     1621 tctcatccaa tgccatcctg gcgctggcgt ccggcggcgg gccggatgat tccggcggtg
     1681 ttccggaagt ttctgtcgtg gcaacgacac caataaccgc ggaacgctcg acacctcccg
     1741 gcgtgttcac cgttactcgg accggcagca caaacggctc cttggccgtg caatatacgc
     1801 ttagcggcac cgccaccaat ggcgtcaact acacaaactt gagcggttcc gtgaccattc
     1861 cggcgggggc cagcactgcg caagtcttga taggtccgat caatctgagc ttctcggacc
     1921 tttcccggac agcgattctc accctgagcg gcagcacaaa ttatacgatg gcccttgcaa
     1981 actcggacac tgtcacaatc caaaataacg acgtggtccc tgtcgcttat gtggcgacgg
     2041 cggacaacgc caaatcaact caaccaaaca ccactgttga tgtctggttt ggcgcggcgg
     2101 tgactttgcc ttcggcgacc aatctttcga attacgcctt gagcggcggt ctgacagtca
     2161 ccagtgctac gctggacaca agccataacc tgagggtgat ccttggagtt tccacgcccg
     2221 ttccaaccaa tacgatattg agtgtctcag gtgtgcttga tgctgccgga aatggcgcgt
     2281 caacaaacat cacaatccgc gtcgggttgc cgtcttgcga tgtggttgcg ctcgtttttc
     2341 accaggggcg gtcaaccggc ttcacaagcg tcacggatgg agtcgtgaat aacgtaaaca
     2401 atgtccctac ggccgggcgc acaggatttg acacatacaa cggcgccggc accgtccagt
     2461 tcggcggcat gatttatcag tcgccagttg atattcaagc catcaaagtg gacttgggac
     2521 aacagttcag cgacggcggg gattgggggt cacaaccgca tgtttatatt ttgaagaatc
     2581 caaaagacac ggactcaacc caaccacagg ttgatacaaa tgattgggtc caagttccgg
     2641 ccgtgctcat cagcggcagt tctcccttcc tgatcagcgg caccttgccc aacccctcgc
     2701 cgaacacgcc ttatgtctat gacctttccg ggttgacgcc cgcgcaacgc tcgggctatg
     2761 gctgggcggt gggcggcgtg caggccagcg gcgcgcacgc attcatgtcg ttcagcgaac
     2821 ttcgcgccta cggcgttgcc tctaccaact atcctacagt gtccttcgca ttgcagccgg
     2881 tgaacgcgac agtcacagcg ggacaacggg ccatattttc ctcccggttg aacatgtcca
     2941 acttgatggc gaccgttctt tatccaccgg gcaacgacgc cggcgtcaat gtccccattg
     3001 tcggttatca atggtacatg gatggctcgg ctgtcactgg agcgaccaat gccacttata
     3061 cgacggacca gaccaccacc gctgaaaccg gcaatcaata ttacctcgtt gcgagcaatc
     3121 atttcttcag catcaccagt tcgacggtga cattgacggt gaacgcccgc acgagtccgc
     3181 cggtggtggt ggcggcaacg gtcgatcttt acgcgaacat cgatgtgtgg tttgacgaac
     3241 cggtggatcc gggcacgtcg cagaaccttg gcaattatgc gctgaatgat cccgcgttga
     3301 ccctgcaaag cgtcacacag aattcatatc aaacacgggc cgccttgaca tacaccggca
     3361 cgcagagcgt cagcaacctg accttgaccg tctcgggcgt tgcagacacg ttcggcaata
     3421 cgcttggttc gcaaacggtt ccattgatac cccaagattg gccggtccaa aatcttgtgg
     3481 ccaatgctta ccaacagggc agggccgcca tgctgacaac tgtgacagac gggcaagtgg
     3541 tcatacacac tgcggccacg tttgctgggg gctctatggg tcaaactcat tttgcgggct
     3601 tgatttataa ccagccccaa gtgttcgggg ttgtcaaagt ggaccttggc aaccagtttg
     3661 cggatggcgg cgactgggcg tttcaaccaa acgtttacat cctcaagcag tccatagacc
     3721 ctaactcaag ccggccggag tccacgccca gcgtgaacgg cgcgccggca tgggtgcaag
     3781 tgccggcaac gctcatcagt ggaaatacct actcatggga gattgacggc ccggtgaaca
     3841 gccttgaagt caatggcccc accgccttcg acttgtcgca tctgcccgct tcgcaacgaa
     3901 cgggttacgg ctgggctgtg ggcggtgtcg ttccaaatgc cctcggatcg gcctacaccg
     3961 gcatcactcc ggcggcggaa ttcctctcgc tctcggaact gcgcggattc ggtgtgacgc
     4021 ccagcgctgt gacaggcgct ccgcaggtcc ttgaggacat cccgtcaact cttgcttatc
     4081 cagaaggttt gccgctcgcc ctttcggtgc tggtgggtgg aacgcaaccc atctcttacc
     4141 aatggaagcg taacggaacc aacctcaccg acggtggacg gatcagcggc tcacaaacca
     4201 gcgccctgaa catcgccgaa gtgcttgccg ccgacgccgg gacttaccag ttgttcatga
     4261 ccaatacatc cggcactgcc agcagcaccg cttcggcgtt gagcatcacg aggattgcgc
     4321 ttaataacgg cgcaggctgg gttcaaaacg gcgcgggtgg aaccaccgac acctacggac
     4381 cgccgatttc aggcaatgtg ctggcattga ccgacggact taccggtgaa acccgtagcg
     4441 cgtggttgaa tgcgccgcaa tatatcggcg cattccaagc ctcctatacg tatcaagata
     4501 tgggtggcgg cggcgcggat ggcgcggcct tcgtgatgca gaattcgacc agcggcacga
     4561 ctgctcttgg cggcggcggc ggagcgctgg gttataacgg aatcactccg agcgcggcgt
     4621 tggagtttaa catctattcc ggcaacacca atggctatgc cttccgcacc aatggcgcga
     4681 cgggcatccc ctacaatcgg acgggagcgc tccgggtgga cagtggcgat ccgatcgggg
     4741 tgtcgctcaa ttacaacgga acggtcttga cgatgacact cacagacgct gtggcgcagg
     4801 tctccttcgc tacaaatttg cccgtcaaca ttccagccat tgtaggcagc aattcagcgt
     4861 acgtcgggtt taccggagca agtggcggcg ttgcctcaac gcagaagatc agcaactttg
     4921 tctttgcgag cctcccgcaa ctctccattc agcccgccag cggcaacaat tacctgttca
     4981 catggccgca gaccgcgggt ggttttgtgt tgctacaaaa ctccaacctg agtacgacca
     5041 actggacggc tgtcaatatt ccgctcgtcg tgaccaatgg acagaatcag gtcactgtcc
     5101 cggtgcaacc gggcaccgaa ttctatcggc tgcggctgcc gtgagttcgg ggttccagcg
     5161 ggaaactgtt tagaaatgcg cgaacagtgg ttactggctg tttcgagtgg aaatgacagg
     5221 cgagacggtc gtttcttatt cgtagcgcag atttttaatc tgctgtaccg ccggcttgca
     5281 gccggcaggg catgataatc acgaggcgcg tgaagatttt tcgcagctcg ttgcgacttt
     5341 tcagcgccag cagattggaa atctgcgata cagcaggctc caagcctgcg ctacgaagcg
     5401 gaacagcgat atctcagaat caaaccacat aaaaatcaaa ttgaagcatc tcacccgaaa
     5461 atttttcttc cgttcaaaat tggagatgct ctttctcgcg gcgtgcatcg gcatttgcaa
     5521 ttcggccggc gctgcggcca cggaaccgcg cggattggac gcaatcacgc aatttgaccg
     5581 gctgccgtat ttgaaaccgg acacgatgga ggaaggccag tcgagttttg accgttcggg
     5641 aggcaacagc gatttcggca attttcttta ccgggatccc gacggcacaa acaaagtgct
     5701 cctggacgtg gccgggccgg gcacggttta tcggatatgg tttaccggct tcaatcaggc
     5761 aaccgactac atcaaggtct atttcgatgg cgaaactacg ccaagaattg acatgctttt
     5821 gaacgatttg tatgccggga ccaacgcgcc ttttctgtcg cccttggtgg cgaacaacct
     5881 tgtttccagc ggaggatttt attgttacct gccgctcccg ttcaatcgct ccatcaaaat
     5941 tgtctccaat gccaccgccg gttcgtttta ctacaacatt ggctatcatg tttatagcca
     6001 ggacacgacg gtgaccacat ggaccggttt ggaagattcg actgcggcgc gaaatctctg
     6061 gaaaaatgtc ggcagcgatc ccaagagcga cagcggaaat gtggttgttt ccaacgcgtt
     6121 caatctggct ccatcggggt cgcagacact attggacata tctggacctt attcgatttc
     6181 ctcgatcaaa ttgaactttc ccgggattaa ttttgcagcg ccgctcacca tcaccgatga
     6241 cgggcgcgcc gacaagggct acagccaatt tcagatggcc atcaacccga caaatacagg
     6301 cgtcgagttg gtgcggcgat tggactacgg gatcggcaat caaaaggcca atgtttatgt
     6361 ggatggaacc ctgatcgggc aatggtttga cgcggggtcg gaccaaacat atcactggcg
     6421 ggacagcggt ttcaacatcc cctcaactta tacgacgggc aaaagcgcga tcaccgtcaa
     6481 agtgtcctat gtaagctcgg acgtggattg gaatgaattt tactattggg catactcttt
     6541 gagcgccgca tcgagcaatc ttaccgactc gctcgatgtc ggcaactcat cgtctgagac
     6601 aaaccacgcc tacgtgatca acacacaaac gtggtctggc gttcgcacgt ttcaataccc
     6661 gccgggcgag gacccaaaca cgatcgaact gctgaccaac acatggcttt ccatctcatt
     6721 tgataatgag acaaatccca gtgtttacgc gccggtgagt tcattctttg ccatgggcca
     6781 attcgcgccg tacccagtcc gcgccttgcc tgtcggcgtg gatgccaaca gcaatcttta
     6841 ttgttatttt cccatgcctt ttggaagcca cgccactgtc caactcgtga gctttcgcgc
     6901 cgtgacaacc tccaacatca cctgcgagct gcgatacaaa ccattcacgg acagccttgc
     6961 cagggttgga tatttcaaga cgcagtataa ctccgagctt ccaaccacga acggcgcgga
     7021 catcgtcatg ctggatgccg aaggcaccgg ccattttgtc ggggttgttg aatccatgat
     7081 gggagtggtg agccgctatt atctagaagg cgatgagcgc atttacgttg ataacagcgc
     7141 aagccccgcc atttacggaa ctggcaccga ggatttctac gacgcgggtt ggtacttcca
     7201 gaacgggctc ttctctctgc ccattaatgg caaccccgcc catgtggccg atgcgaatta
     7261 tgaccgcaca accgcctatc gtttttttct gtcggacgcg atcccgttcc gcaagcacat
     7321 caccgtcggg attcaacacg gtggcgtcga tgattcatcg gagaacgtct ggactttggc
     7381 ctactattat tgccagccaa cgaaccacgc gattttaacc gaccaattgc tggtggggaa
     7441 cactcccagc gaagccgcgc actcttattc catcaacact cagacatgga cgggaacacg
     7501 cagctacacc tttgatggca attttgacac tgtgatcgtc acgaacaccg ggcgggcgca
     7561 caaaggttac agtcaattca ccatggcgct ctcgcccaca aatgcaggcg caattttgcg
     7621 gcgtcaattc gatcagggag ttgccaatca aaaagcgaac gtctatgtgg atggcgtgct
     7681 tgcgggcgta tggtaccggg ccggaagcaa taccattcat aattggcggg atgatgattt
     7741 tatggtcccg tccgccttca cttcgggaaa aaatgcgatc caggtccaag ttcaattttt
     7801 atcgtcgagc aatgattgga atgaattcgt atattccatt tattcgcttc ccatcatcac
     7861 gaatgcgacg cctccccgcc tgattggcgg cacttggaga ggcaatgctt ttgccatcag
     7921 cttgtcgggc gatccgcata ctggttacgg cgtgtgggcc tcaacgaatt tggccaattg
     7981 gggttggctt ggcacggcca gcgaaacgag cccgggaatt tatggcttta ctgacaccac
     8041 tgcaaacggc attccgcaac gcttttaccg tgggaagtcg ccttgaaatc gaaattgccc
     8101 ggaccccaag ttgctgcgtc agcgacccgc gctccggcca tcctgtttct ctttccaacg
     8161 gctgggggcg acgacaccgg tttttccgaa atgacggatg cgcagtgcgc gcgggccatc
     8221 gccctgttca attggcgaaa aaacaagccg accaaattgc gttggttcga gcagttgccg
     8281 aaatacggcg agtgaatcat aaaatcactc cggattgaag gacgttcata ggggcttttc
     8341 cccatttgaa aggcgttttt gccggggcta attttgcccg cgaggtcaac cggaactctt
     8401 agagcgaacc attcctgttt gtcgttatga ccgggctgag cgttttcgcg gaaccctgcg
     8461 cgaatgcttg tatgaacaga ggtggacgaa acgccgggcc gggaataggg gaccgcaagg
     8521 acgcgcgcca cccggaacca gaagaggaga aggaccatcg tcccgaggag aaaagttccg
     8581 aggaaaaaga cgatgcggcc aaacaagggc cgcaaaggcc gcccgcttac aagcggccgg
     8641 gatttctcat catagtttcc gttgtgctgc ttgttctcat cgtgggcggg cttctctatt
     8701 ggttgcacgc gcgacatttt gtttc
//
LOCUS       DASZMM010000476         8672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1605301, whole genome shotgun sequence.
ACCESSION   DASZMM010000476 DASZMM010000000
VERSION     DASZMM010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605301"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VG754_11410"
     CDS             complement(<1..635)
                     /locus_tag="VG754_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWF19870.1"
                     /translation="MNWSLVYLISEWIIRLVMLVYVPQRRSAAASRTWLLLIFLLPWP
                     GLILYAIFGRIYVSKRRIALQLRASQHIRAVQTQMGERAFAQSKLPPDAATIALLASR
                     LGDFEPFGGNNIELITDYDAAIDRLIADINSARHHVHLLHYIYGDDEIGRKVAAALKE
                     AVKRGVECRVLMDAVGSKEALARLAPKMRAQGIEVHAMLPVNFLHRNAARF"
     gene            918..2375
                     /locus_tag="VG754_11415"
     CDS             918..2375
                     /locus_tag="VG754_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF19871.1"
                     /translation="MLTDDQMQTQKPSPAQEPKRVHAPQSPRIIDPKPIDSSKKSSKV
                     IWVVVALFCIVAGLAFWGVKRRQATAAMAGAKFGQQPVVPILAGVVEEKNVPIYLDGL
                     GTVQAYNTVTVRVRIDGELKKVAFTEGQDVHAGDLLAQIDPAPYKSALDQAKAKKAED
                     EAQLTNALVNLKRETELLAAKIDAQQVYDAQKTLADQMDATVKADEAAIESAQVNLDY
                     CTITSPLGGRTGIRLVDQGNMVHATDSNGLVVITQLQPISVIFTLPEQDLDKIHREMP
                     ENGDLKVLAIDRDNNKTLDEGTLAVIDNQIDTSTATIRLKANFPNAKNRLWPGEFVNP
                     RLLLTVRSNGLVVPTVAIQRGPTGAYVFVIEEGGAGSGKSGKGKRPDGDSVASDNAPA
                     QASKQNSDSQGGTGGQGGHSGGDGKQPALHVKMQAVTVAAEVTGTESLITAGLQKGQR
                     IVVDGQYKLQDGSPIRIVSADKAGSVDSRSSDSMQ"
     gene            2372..5521
                     /locus_tag="VG754_11420"
     CDS             2372..5521
                     /locus_tag="VG754_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWF19872.1"
                     /translation="MNISEPFIHRPIATSLLMAGVIIMGMLGYFMLPISALPPVDFPT
                     IQVTAQFPGAGPDVMASSVTTPLETQFGQISGLQQMTSVSSFGTANITLQFVLDRDID
                     AAAQDVQAAMNAAQGVLPTGMPSPPIYSKVNPADTPILTLEITSDSLPLDKVNDLADT
                     VLAQKLSEVSGVGLVTIQGNQKPAVRVRINPAAVAALGLSLEDVRTALVNNNINIPKG
                     SFEGPRQSYSIGANDQIFSASEYANVIIAYKNGSPVRLKDIGDSIDNVENTHLAAWVG
                     KQPAVLLDIQRQPGANIIQTADRVKALLPKLKTSIPPAVKVSILTDRTETIRASIADV
                     QFTLILTVALVVMVIFVFLRKFWATVIPSVALPLAVIGTFGIMKLAGFSLDNLSLMAL
                     TISTGFVVDDAIVMIENIVRFIEAGEPPLQAALKGAKQIGFTVISLSISLVAVFIPLL
                     FMTGIVGRLFREFAITLSVAVGVSAIVSLTLTPMMCARFLQPEDESKHGKFYHATERM
                     FQGMLNAYDSGLKWVLRHQFFTLMVAVVTLIATIWLYIIVPKGLLPQQDTGLIVGVTD
                     ASQTISFQAMVARQHLIADAVSRDPDVAQVASFVGAGTVNPTMNSGRLYINLKPREQR
                     KSDATEIMARLREATRDIEGISLYMQAAQDVQIDSRVSRTQFQYTLQDADSAELNEWA
                     PKLLTAIDKLPELADVASDQQTSGLQLFIDVDRNQASRLNILPAAIDGTLYDAFGQRQ
                     VSIIFTQLNQYRVILESQPDFQLTPAALDKIYVKSTTGQMVPLSTIAHLKTVNAPLAI
                     SHEGQFPAITLSFNLSQNSSLGAAVNAIQQAQKEIGMPQTVTTTFSGSAAEFRSSLKS
                     EPYLLLAAIVVIYIVLGVLYESYIHPITILSSLPSAGVGALLAMLIFHQDLSLIALIG
                     IILLIGIVKKNAIMMIDFALEAERVEGLLPEQSIYKACLLRFRPIMMTTMAALLGALP
                     LALENGTGSELRKPMGISIVGGLLLSQFLTLYTTPVIYLYLDRVERRMRKWLGSPEMK
                     PNEAKPGIRPEPDADLLPS"
     gene            5518..>8672
                     /locus_tag="VG754_11425"
     CDS             5518..>8672
                     /locus_tag="VG754_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF19873.1"
                     /translation="MSISEPFIRRPVGTSLLAIGLFVMGIVAYRFLPVASMPRVDSPV
                     ISVSAGLPGADPATMASAVAAPLEKRLGQISGVNEMTSVSTLGGAGVTLQFDLNRNID
                     GAARDVQAAINASSSDLPLDLPGPPTYRKVNPADAPIMILAMTSENMPVEQVFEYGNN
                     IVGQKLSQVEGVSQAFIGGGAKSAVRVQINPAALASTGLSLEDVRTMLQQVNVDSPKG
                     GFDGRNRAYAIASNDQLREAQAYQNLILSQPNGAPIQLNALGNVVEGVENNFQAGWAG
                     TNRAVLVIIFKQADANVIETVDRVKATLPLIQSWLPPSIKIFPLSDRTVTIRSSVNDV
                     QFALLVSIALVVMVIFLFLRRFWPTFIASITVPLALAGTFGGMYLMHYSLDNLSLMAI
                     TISVGFVVDDAIVVIENVFRFIEHGDTPMVAALKGARQIGFTVVSMSLSLVAVFIPLL
                     FMGGLIGRNFHEFAVTLTMAILVSGVISLTLTPMLCSRFLKNEKDYPPPGAFYRTCEG
                     VFNFFLSGYSRGLRWVLENQAFMLLVWLFTLIGTVCLYVIVPKGFVPQQDTGTLMGTT
                     EAAQDISFAAMAGLQSRVAKVILGDPAVGTVGSFIGATSGSSTVNNGRMFITLKPLNK
                     RGIYVDPGIEKLPPVKRWFAHLLAGRKPVSADQVINRLRPQIARIVGINLFLQAVQDV
                     RVGGRMGKAQYQYSLTSPDLTDLNHWSLALANELSKYPELKDVSTDQQTRGLQANAVI
                     NRDTASRLGVSPTVIDNTLYDAFGQRQVSVIYDQYNQHHVVMEVDPNFQLSPASLNKI
                     YVKGTNGTLSVPLSAVAKFKPSNAYLSVNHQGQFPAVTVTFNLAPGVSLGQATELIQK
                     ATQDLRMPSSVQGSFQGTAQVFKDSLSTMPLLALAALIAVYIVLGMLYESLIHPWTIL
                     STLPSAGVGALLALMICGLDLSLISFIGIILLMGIVKKNAIMMIDFALDVERNERLSP
                     EDAIYKACVVRFRPIMMTTMAALLGSLPLALGGGSGSELRRPLGIAVVGGLLLSQVLT
                     LYTTPVIYLMFEHLRAWVRSWR"
BASE COUNT         1858 a         2438 c         2418 g         1958 t
ORIGIN      
        1 tcgaaccgcg cggcgttgcg atggagaaaa tttaccggca gcattgcatg gacttcgatg
       61 ccttgcgcgc gcatctttgg agcgagccgc gccaaggctt ccttggagcc gacggcgtcc
      121 atgaggacgc ggcattctac accgcgtttg accgcttcct tgagggcggc ggcaactttc
      181 ctgccgattt catcgtcgcc atatatataa tggagcaaat ggacgtggtg ccgcgcggaa
      241 ttgatgtcgg caatcaagcg gtcgatggcc gcatcgtaat ccgtgatgag ttcgatgttg
      301 tttccgccga acggctcgaa gtcgcccagc cgggacgcga gcaatgcgat ggtggcggca
      361 tcgggcggca atttggattg ggcgaaggcg cgttcgccca tttgggtctg cacggcgcgg
      421 atatgctggg aagcgcgaag ttgcagggcg atgcggcgtt tggaaacata gatgcggccg
      481 aaaattgcgt agagaatcag cccgggccat ggcaggagaa aaatcaaaag caaccaggtg
      541 cgcgatgccg cggcgctgcg gcgttgcggc acataaacca gcatgaccag ccggatgatc
      601 cattcgctga tgagatagac caggctccag ttcatgtttg ccatggaatg atgttaggca
      661 aaaaattgcg aaaagaccac agcgagaata tacgattttc gatatgcgat tttcgcggtt
      721 ttcaattgtt tgcttggcgc tttggccgac gcgacctcgt tcccgaagtg aaaatctcag
      781 tcacggcgcg taaatcgaat atcgtaaatc gtatattccc aagtagttgt cagagtggaa
      841 ctaaatatgt aacttattgg ccttatcagc ctcttaagag tagcgatatt gtatcgcttt
      901 actggaatgt ccggattatg ctgacagacg atcaaatgca aacacagaag ccgtccccgg
      961 cgcaggaacc aaaacgcgtc catgccccgc aatcaccaag gatcattgat ccaaagccaa
     1021 tagactcctc aaaaaaatct tccaaagtca tttgggtggt ggtggccctg ttttgcattg
     1081 tggcaggact ggctttttgg ggagtgaaac ggcgtcaagc gaccgctgcc atggcgggcg
     1141 cgaaatttgg acagcaacca gtcgtgccga tcctcgcggg agtggtggaa gaaaaaaatg
     1201 ttcccattta tctggacggt ttgggaacgg tgcaagcgta caacactgtg actgtgcgcg
     1261 tccgcattga cggcgagctg aaaaaggtgg cgttcactga gggacaggat gttcacgcgg
     1321 gcgatttgct cgcgcaaatt gatcccgctc cttataaatc cgcgctcgac caggcgaagg
     1381 ccaagaaagc cgaggacgaa gcgcagttga ccaatgcgct tgtcaatttg aaacgcgaaa
     1441 ctgaattgct tgccgcaaag atcgacgcgc agcaggttta tgacgcgcaa aagacgctcg
     1501 cagaccaaat ggacgccacg gtcaaggcgg acgaagcggc cattgagagc gcgcaggtga
     1561 atcttgatta ttgcacgatc acatcgcccc ttggtggccg caccggcatt cgcctcgtgg
     1621 accagggcaa tatggtccac gcaactgatt ccaacggctt ggtggtcatc acacaactac
     1681 agcccatctc tgtgattttc acattaccgg aacaggacct ggacaagatt catcgcgaaa
     1741 tgcccgagaa tggggatttg aaagtgctcg cgatagatcg cgacaacaac aaaactttgg
     1801 acgagggaac gttggctgtc attgacaacc agatcgacac ctcgaccgca accatccgcc
     1861 tcaaggcgaa ttttcccaac gccaaaaatc gtctctggcc tggtgaattt gtgaatccgc
     1921 ggcttttgct caccgtgcgt tcgaatggcc ttgttgtgcc taccgttgcc attcaacgcg
     1981 gaccaactgg cgcttatgtt ttcgtcattg aagaaggcgg cgctggttct ggcaagtctg
     2041 gaaaagggaa acggcccgac ggtgattctg tggcttcaga caatgctccc gcgcaagctt
     2101 cgaaacagaa ttccgacagt caaggcggca caggcggtca aggcggccat agcggaggcg
     2161 atggcaagca accggcgttg cacgtgaaaa tgcaggcggt caccgtcgca gcggaagtca
     2221 ccgggacgga atccttgatc acggccggtt tgcaaaaggg gcagcgcatc gtggtggatg
     2281 ggcaatataa attacaggac ggttcgccga tccgcattgt gtccgcagac aaggccggga
     2341 gcgttgattc gcgttccagc gattccatgc agtgaacatc tcagaacctt tcattcatcg
     2401 gccgattgcc acttcgttat tgatggcggg tgtcatcata atgggaatgc tcggctattt
     2461 catgctgccg atttccgcgc tgccgcctgt ggattttccg accatccagg tgacggcgca
     2521 attccccggc gcgggaccgg atgtgatggc ttcttcggtc acaacaccgc tcgaaaccca
     2581 attcggccag atcagcggat tgcagcagat gacttcggtc agctctttcg gcacggcgaa
     2641 catcacgctt caattcgtgc tggaccgcga catcgatgcc gccgcgcagg acgttcaggc
     2701 cgcgatgaac gccgcgcaag gcgttctgcc cacgggcatg ccgagcccac ctatttacag
     2761 caaggtaaat ccagccgaca cgccgattct cacgctcgaa atcacctccg attcgttgcc
     2821 gctggacaag gtcaatgacc tcgccgatac cgtgctcgcg caaaaactga gcgaagtcag
     2881 cggcgtcggt ttggtgacca tccagggcaa tcaaaagccc gctgttcgtg ttcggattaa
     2941 tcccgctgcg gttgcggcgc tagggctaag tcttgaagat gtgcggaccg cgctggtgaa
     3001 caataatatc aacattccca agggaagttt cgagggcccc cgccaatcct attccatcgg
     3061 cgcgaacgac cagatttttt ccgcgtcgga atatgccaac gtcatcatcg cgtacaaaaa
     3121 cggttcgcct gtccggctca aggacatcgg ggacagcatt gacaacgtgg agaacacgca
     3181 tctcgccgct tgggtgggca aacaaccggc ggtgctgctc gacatccaac ggcaaccggg
     3241 cgcaaacatc atccagaccg ccgatcgcgt gaaggcgctt ttgccgaagc tcaagacgtc
     3301 cattccgcca gcggtgaaag tttccattct caccgaccgc accgaaacca tccgcgcctc
     3361 cattgccgac gtgcaattca ctctcattct cacggtagca ttggtggtga tggtgatttt
     3421 tgttttcctg cgaaaattct gggcgacggt gattccaagc gtcgcgctgc cgctggctgt
     3481 catcggcacg ttcggcatca tgaagctcgc cgggttcagc ctcgacaatc tttcgctgat
     3541 ggcgctgacc atttccaccg gtttcgtggt ggatgacgcc atcgtgatga tcgaaaacat
     3601 cgtgcggttt atcgaagccg gtgaaccacc gttgcaagcc gcactcaagg gcgcgaaaca
     3661 aatcggtttc accgtcattt cgctgagcat ttcgctggtg gcggtcttca ttcccctgct
     3721 cttcatgacc ggcattgtcg gacgtttgtt ccgagaattt gccatcacgt tgagcgtcgc
     3781 cgtgggcgtg tcggcgattg tttcgctgac gctcacaccg atgatgtgcg cgcgtttttt
     3841 gcaacctgag gacgaaagca agcacggaaa attttatcac gcgacggaac ggatgttcca
     3901 gggaatgctg aacgcttatg acagcggtct caaatgggtg ttgcggcatc agttttttac
     3961 tttgatggtc gcggtcgtga cgctcatcgc caccatctgg ctctacatca ttgtgccgaa
     4021 aggattattg ccccaacagg acaccggact gattgtcgga gttactgacg cgtcacaaac
     4081 gatttctttc caggcgatgg tcgcgcggca acatctcatt gccgatgctg tcagccggga
     4141 ccctgatgtg gcgcaggtcg cgtcgttcgt cggcgcgggc acggtgaacc cgacgatgaa
     4201 cagcgggcgg ctctacatca atctcaagcc gcgcgaacaa cggaaatcgg atgcgactga
     4261 aatcatggcc cggttgcgcg aggcgacgcg cgacatcgag gggatttcgc tttacatgca
     4321 ggccgcacag gatgtgcaga tcgacagccg cgtgagccgc acccaattcc aatacacatt
     4381 gcaggacgcg gactcggctg agttgaacga atgggcgccc aaattactca ccgcgatcga
     4441 caaactccct gaattggccg acgtcgcctc cgaccagcag acgagcgggt tgcaactttt
     4501 tattgacgtg gaccgcaacc aggcttcgcg cctgaatatt ttgccggctg caattgacgg
     4561 gacgctttac gacgccttcg gccaacggca ggtttcgatc attttcacac agctcaacca
     4621 gtatcgcgtc atcctcgaat cgcagcccga ttttcaactt acgcccgcgg cgcttgataa
     4681 aatttatgtc aagtccacga ccggccagat ggtgccgttg agcacgattg cgcatctcaa
     4741 gacggtcaac gcgccgcttg ccatcagtca tgaaggacaa tttcccgcca tcaccctttc
     4801 attcaacctc agccagaaca gttcgttggg cgcggcggtc aatgcgattc agcaggcgca
     4861 aaaggaaatc gggatgccgc agacagtcac caccactttc tccggaagcg cggcggaatt
     4921 ccgttcgtcg ctcaaaagcg agccttacct gctgcttgcg gcaatcgtcg tgatttacat
     4981 cgtgctcggc gttctttacg aaagctacat ccatccgatc acgattttgt ccagccttcc
     5041 gtccgccggc gtgggcgcgt tgctggcgat gctgattttt catcaggacc tttcgctgat
     5101 cgcgctcatc ggcatcatcc tgctcatcgg cattgtgaag aaaaacgcca tcatgatgat
     5161 tgacttcgcc ctcgaagccg aacgcgtaga aggcctgctt cccgaacaat ccatttacaa
     5221 ggcctgcctg ctgcgttttc gtcctatcat gatgacaacg atggccgcgt tgctcggcgc
     5281 gttgccgctt gcgttggaaa acgggaccgg ttcggaattg cgcaagccga tgggcatttc
     5341 catcgtgggc gggttgcttc tttcccaatt cctcacgctc tataccacgc cggtcattta
     5401 tctttatctc gaccgtgtgg aacggcggat gcgcaaatgg ctcggttctc ccgaaatgaa
     5461 accaaacgag gccaagcccg gcattcgccc ggaaccggat gcggacctct tgccgtcatg
     5521 agcatttcgg aaccgttcat tcgccgtccg gtcggcactt cgctgctggc gatcggcttg
     5581 ttcgtcatgg gcatcgtggc atatcggttc ctgccggtgg cgtccatgcc gcgcgtggac
     5641 tcgccagtga tctccgtttc ggcgggtctt cccggagctg acccggcgac gatggcgtcc
     5701 gccgtggctg cgccgttgga aaaacgtctg gggcagattt ccggcgtaaa cgagatgacg
     5761 agcgtcagca ccttgggcgg agcgggtgtc acgcttcagt ttgatttgaa ccgcaacatt
     5821 gatggcgcgg cgcgcgatgt ccaagccgcc atcaacgcct cgtccagcga cctgccgctt
     5881 gaccttccgg gaccgcccac gtatcgcaaa gtcaatcccg ccgacgcacc catcatgatt
     5941 ttggcgatga cttcggaaaa tatgccggtc gagcaggttt ttgaatacgg aaacaacatc
     6001 gttggccaaa aactgagcca ggtcgaagga gtcagccagg cgttcatcgg cggcggcgcg
     6061 aaatccgctg tgcgcgtgca gatcaatcct gcggctttgg catccaccgg cttgagtttg
     6121 gaggatgtgc gcacgatgct gcagcaagtc aatgtggatt ccccgaaagg cggatttgat
     6181 ggccgcaacc gcgcatacgc catcgccagc aacgaccaac tccgggaagc gcaagcgtat
     6241 caaaacctca tcttgagcca gcccaatgga gcgcccatcc aattgaacgc gttgggcaac
     6301 gtggtggaag gcgtggaaaa taattttcaa gcagggtggg cgggaacgaa ccgcgcggtg
     6361 ctggtcatta ttttcaagca ggccgatgcc aatgtgattg aaacagtgga tcgtgtaaag
     6421 gccaccctgc ccctcatcca aagctggctg ccgccttcga taaaaatttt cccattgagc
     6481 gaccggactg tcaccatccg ctcttccgtc aacgatgtgc aattcgccct gctggtgagc
     6541 atcgctctgg tggtgatggt catttttctt tttctgcggc gtttctggcc gacattcatc
     6601 gccagcatca ccgtgccgct agcattggca ggaactttcg gcggcatgta cctgatgcat
     6661 tacagcctgg ataatctttc gctgatggcc atcacgattt cagtcggttt cgtcgtggat
     6721 gatgccatcg ttgttattga gaacgtcttt cgtttcatcg agcacggaga cacgccgatg
     6781 gtggcggcgc tcaaaggcgc gagacaaatc gggttcacag tggtttccat gagtttgtcc
     6841 cttgtggccg tgttcattcc gctgttgttc atgggcggat tgattggccg caattttcac
     6901 gagttcgccg tgacgttgac aatggccatt ctcgtctcgg gcgtgatttc actcacgctc
     6961 acgccgatgc tctgctcgcg ttttttgaag aacgaaaaag attatcctcc tccaggcgcc
     7021 ttttaccgga cctgcgaagg ggtttttaat tttttcctga gcggctattc gcgcggcctg
     7081 agatgggtgc tggagaacca ggctttcatg ttgcttgtgt ggttgttcac gcttatcggc
     7141 actgtctgcc tttatgtgat cgttccgaaa ggcttcgttc cgcaacagga caccggcacg
     7201 ctgatgggaa ccacggaagc cgcgcaggac atttctttcg ccgcgatggc cggccttcaa
     7261 tcccgcgttg caaaagtcat tcttggcgat ccggccgttg ggacggtcgg ttccttcatc
     7321 ggcgcaacgt ccggcagttc caccgtcaac aatggccgca tgttcatcac gctcaagccg
     7381 ctcaataaac ggggaattta cgttgacccg ggcatcgaga aattgccgcc ggtgaagcgc
     7441 tggtttgcgc atttgctggc cgggcgaaaa ccggtgagcg ccgaccaggt catcaaccgg
     7501 ttgcgtcctc aaattgcgcg gatcgtcggc atcaatcttt ttctccaggc ggtgcaggac
     7561 gtgcgagtgg gcggacgcat gggcaaggcg caatatcaat attcgctcac gtcacccgat
     7621 ctcaccgacc tcaatcattg gtcgctcgca ctggcgaatg aactcagcaa atatccggaa
     7681 ctcaaggatg tatccaccga ccaacaaacc cgtggattgc aggcaaatgc cgtgattaat
     7741 cgcgataccg cctcgcgcct tggcgtttcg ccaaccgtca tcgacaacac gctttacgat
     7801 gccttcggcc agcggcaggt ctcggtgatt tacgatcaat acaatcagca ccacgtcgtg
     7861 atggaagtgg acccgaattt tcaactgagt cccgcgtcgc tcaacaaaat ctatgtcaaa
     7921 gggaccaacg gcacattgtc cgttccactg tccgcagtgg cgaaatttaa gccgagcaac
     7981 gcctatcttt ccgtcaatca ccaaggccaa tttcccgccg tgacggtcac gttcaatctc
     8041 gcgccgggcg tttccttggg acaggcgact gaattgatcc agaaggccac gcaagatttg
     8101 cgcatgccgt ccagcgtcca gggaagtttt cagggcaccg cgcaagtttt caaagattcg
     8161 ctttccacca tgccactgct tgctctcgcg gcgcttatcg ccgtttacat cgtgctcggg
     8221 atgctttacg aaagcctgat tcatccctgg accattttgt ccaccctccc ttcagcgggc
     8281 gtgggcgcgt tgctggcctt gatgatttgc ggcttggacc tttcgctcat ctcgttcatc
     8341 ggaatcattc tcctgatggg catcgtgaag aaaaacgcca tcatgatgat tgacttcgcc
     8401 ttggatgtgg aacgcaacga gcgtttgagc cccgaagatg cgatttacaa ggcatgcgtc
     8461 gtccgcttcc gccccatcat gatgaccacg atggcggcgt tgcttggttc gctgccgctc
     8521 gcgctcggcg gaggaagcgg ttctgaactg cgccgtccgt tgggcatcgc cgtggtcggc
     8581 ggattgttgc tttcgcaagt cctgacgctt tacaccacgc cggtgattta cctgatgttc
     8641 gaacacctgc gcgcgtgggt gcgttcatgg cg
//
LOCUS       DASZMM010000477        22055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_501951, whole genome shotgun sequence.
ACCESSION   DASZMM010000477 DASZMM010000000
VERSION     DASZMM010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 22055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22055
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501951"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..624
                     /locus_tag="VG754_11430"
     CDS             79..624
                     /locus_tag="VG754_11430"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HWF19874.1"
                     /translation="MATVLMLSILSGLLLAGFAAYADESAPSEYQVKAAFLYNFAKFV
                     KWPESAFAQTNSPIKIGILGEDPFGGNLDLAIKGKTINGHPLSVIELSSPDEMKNFQI
                     IFVCRKPKRNLVATLAHLNNASVLTVSETDDFLKAGGMIKFVMENARVRFEINDSAAT
                     RSGLTISSKLLNVARKRESND"
     gene            629..3142
                     /locus_tag="VG754_11435"
     CDS             629..3142
                     /locus_tag="VG754_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19875.1"
                     /translation="MKALRHISINRKLTLIIMVASTVALLLISASFVTYELITFRQRM
                     RTELSSLATFIAIQNDAALLYGKNTDAYDSLGALSAKPHIVSACVYDAQGKVFATYPA
                     NISHTLLPKRPGTDESWFENDHLKLFHSIVKDDDIIGTVYLESDLNELHQRLLQYGGI
                     ILLFILGSLLITFLVSSFLRNIISEPIAHLARTARAVSAEKKYSVRAVKLSEDELGQF
                     VDVFNEMLSQIQVRDAALQQANDELEKRVEERTRDLKSEIAERQRAQDGLQQQLGRIS
                     LLNHIAQVISDRQDLTSILHVVLRQLEDHLPVDLGGVWLLNPQGDALTVAALREKNPF
                     GPVQPLLKNGEMLPLEPGGLSRCKLGETLYWADTMELSAQLAQRLSGAGLRCAVSVPL
                     MVEGKLFAILSVARQTANSFTSGECEFLKMLSEHVALAAHQAKLHTELESAYNDLRHS
                     QQAVMQQDRLRALGQMASGIAHDINNALSPVVGFAGLLLEYEPNLSDSARKNLTYIRT
                     AGEDVAHIVARMREFYRQRDKQEILFPLSLNELAEQVIDMTRPRWRDIPQGRGIMVET
                     QTDFAKDMPQVAGIEAEVREALTNLMLNAVDALPSGGKLIVRTSTPYLPTTEQAKNTP
                     KHGVLEVIDNGTGMSELTLKHCLEPFYSTKGQRGTGLGLAMVYGVMERHQGKIEIESE
                     LGKGTTVRLIFPLLAERPAADTNGKTIAPLRPLRILSIDDEPLLRELVKEMLERDGHH
                     VQSVDGGQAGVDMFHKAMEKHHPFDVVLTDLGMPFVDGREVARLLKKASPQTPVVLLT
                     GWGAFMKRDGEVPAQVDGVLSKPPRLNELREMLAHVTQG"
     gene            complement(3278..3652)
                     /locus_tag="VG754_11440"
     CDS             complement(3278..3652)
                     /locus_tag="VG754_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWF19876.1"
                     /translation="MAQVPVISEAEWKVMKVLWTNSPLSAMEIFNALADTEDWHPNTV
                     KTLLSRLHKKKALGVEKVKNLYLYRPLVSEADCVRTESASFLHRVFGGSVKPLLMHFM
                     EKEPLSSQDLDELRKILRQRGK"
     gene            complement(4060..4971)
                     /locus_tag="VG754_11445"
     CDS             complement(4060..4971)
                     /locus_tag="VG754_11445"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF19877.1"
                     /translation="MRTLIIGCGYVGTALGAELARQGHEVFGVRRASEGAAELTAAGI
                     KPLIADVTKPAELAALPGPFDWVVNCVSSTRGGVEEYRAVYLQGTRNVIEWLAAAPPK
                     KFVYTSSTSVYGQTDGSSVKETSSTDPASETSRVLLETEKILLEAAQQKKLPAVVLRV
                     AGIYGPERGHLFLQYLKNEAKIAGQGERIINMIHRDDLVGVIVAALKNGRAGEIYNAV
                     DDEPVAQIHFFRWLSETLGKWMPPFATEEESAERKRGLTNKKVQNRRLKMELGYQFKY
                     PNFRKGYTAEIQRLDQAGLLQIAPEPR"
     gene            5086..5880
                     /locus_tag="VG754_11450"
     CDS             5086..5880
                     /locus_tag="VG754_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HWF19878.1"
                     /translation="MSTQQPSTSSAPIKTFQADALPVRVYASQTELSQDVARIAQAHL
                     REVLAAKGSAAVILATGNSQIKFLEELIRIGGVEWSEITLFHMDEYLGISAEHKASFR
                     LYMRERVVSRIKPRVFHYLEGDALLPMDECDRYTALLRAQPIDLCCLGVGENGHIAFN
                     DPPVANFEDPRAVKIAKLDEACRMQQVGEGHFANMGAVPQYALTLTIPMLCSAKKMLC
                     IAPEKRKAQAIKDALQGPISTACPASFLRRQAHCTMFLDRDSTSLL"
     gene            5951..6706
                     /locus_tag="VG754_11455"
     CDS             5951..6706
                     /locus_tag="VG754_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWF19879.1"
                     /translation="MLSEKPWNWDRVVRVFIAVLFFVSVFFLVMAATQHYAGKTKLVE
                     GSLAYVVLGSLGFHGSILFGTAIILASDHLNWGKAFGFSSRPLTRAMLLGFAAALVFL
                     PVGMVLQDVSLRALDWFHVPTPPQPAVEQFDKATSVATRVYLAFFAVVLAPVAEEIFF
                     RGILYAAIKQFGFPRLALWGSALIFATIHLTPAIFVPLLLLSLILVWLYEKTDNLLAP
                     ITAHAVFNGINLLLMYYSDDLAHSIQRFFPHFK"
     gene            6703..7635
                     /gene="thiL"
                     /locus_tag="VG754_11460"
     CDS             6703..7635
                     /gene="thiL"
                     /locus_tag="VG754_11460"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWF19880.1"
                     /translation="MNEFELIGRLAASLPSNSSVVKGAGDDCAVLDLGLPERLVLFKT
                     DAVVEGIHFTAETPPEQVGHKALARCLSDIAAMSGTPTAALVTLALPRDFDAAHVEKL
                     YAGINALARRYDVAIAGGETTTNPERIFISIALLGTVPRNECLSRSGARVGDALFVTG
                     ELGGSISGKHLDFEPRLVEARWLTEHFSIHAMIDLSDGLAGDLRHVLRASKVGAELLA
                     SAIPISRSAKIQSKAETATKTPLLAALTDGEDFELLFAVASCDAVPLLDAWKRQFPKL
                     KLSCIGKIITGEGVTIRDKRGTHSFESHGYVHFQ"
     gene            7613..8056
                     /gene="tsaE"
                     /locus_tag="VG754_11465"
     CDS             7613..8056
                     /gene="tsaE"
                     /locus_tag="VG754_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWF19881.1"
                     /translation="MATCISNSPEATLAFGEEWGRAAASGWVIGLSGDLGAGKTQLAK
                     GIARGLGFAGRVHSPTFTLLNQYEGGRLPLFHVDLYRLKTSDQIIAAGLEEYFHPDGV
                     SIIEWPERWTSPRFKVQHSKCFYRRVLIEVISETERRITYEDFGG"
     gene            8037..8684
                     /gene="tsaB"
                     /locus_tag="VG754_11470"
     CDS             8037..8684
                     /gene="tsaB"
                     /locus_tag="VG754_11470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWF19882.1"
                     /translation="MKILAVEFSSEERSVAVATDGNIRGSAMEKATRATHAFALIEQA
                     LTQARIEREQIECLAIGLGPGSYTGIRSAIALAQGWQLALPVKLLGISTVECLAAQAQ
                     SQGWFGKVNIVIDAQRDELYFARYEISSHGYREIASLRLATPDEVRLQCKSDEIIAGP
                     DANRWFEKGRIIFPDAATLARLAASRTDFMPGEKLEPIYLRETNFVKAPPPRIFG"
     gene            complement(8685..8918)
                     /locus_tag="VG754_11475"
     CDS             complement(8685..8918)
                     /locus_tag="VG754_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19883.1"
                     /translation="MKRSLILPVGMLALTLLLENGCVVAAGPPGPPPPPSPVVEYYGP
                     APYPGAIWIEGGWRWHPERGRYEWHHGEWHHRG"
     gene            9075..10106
                     /locus_tag="VG754_11480"
     CDS             9075..10106
                     /locus_tag="VG754_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWF19884.1"
                     /translation="MKAAVLYGQEQIRVEEVAPQPLPPGTVRIQIEAALTCGTDLKVF
                     KRGYHARMIVPPAVFGHELSGTISEVHPEAAGWKTGERVVVANSAPCGKCFYCGNQQE
                     NLCDDLLFLNGAYAESIVVPARLVQKNLLRLKPETAFADAALVEPLACVVQGVQEAYL
                     RSGQSVLVIGTGPIGLMFIALAQDMGCKVTAAGRGEVRLQTARRLGANCVIDIANQND
                     LARTIQAGAKEPFDVVIEAVGKPEVWEAATRLVRKGGKANFFGGCPSGTTVALDTALL
                     HYSNLTLLASFHHTPRAIRRALELIEKGIIRARDFVDGECPLTQLPQLFKSMANGNRA
                     VKTLIRVRE"
     gene            complement(10152..10760)
                     /locus_tag="VG754_11485"
     CDS             complement(10152..10760)
                     /locus_tag="VG754_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWF19885.1"
                     /translation="MKHFLEPIVRWYLDHLSYSRLALLMAAESTIFPLPSEVIIPPAA
                     ASGKFSVVGIIIAGTLGSWIGASIMYWVSRTAGRPLVAAYGRYVGISPEKIEGAERWS
                     AKFGSMGIFISRLLPVVRHLIGIPAGIVRMDYRLFSLFTVLGSGIWCAILCWLGIKMG
                     QDEQLMKGEYHRIVIWVGGAMVVLGSIYYFFVHRHMKAAPGK"
     gene            complement(10858..11715)
                     /locus_tag="VG754_11490"
     CDS             complement(10858..11715)
                     /locus_tag="VG754_11490"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWF19886.1"
                     /translation="MSDAYLQLLDATIQHLETLKEQGVHFVSVSPENLSVLGQPTARR
                     TVPTSSTAPAVSTIRPHTISPAVAPIFSVATTSDPAKVAAFAELRSRAMACVKCSHLA
                     SSRKNVVFGVGNIESPLIFVGEAPGADEDEQGEPFVGKAGQLLTKIIQATGLKREDVY
                     IANILKCRPDTPGQTAGNRKPTSQEMETCIPFLHEQIDLIKPKVLVALGATAVEGLLG
                     KTIGITRLRGQWQTYRNIPLMPTYHPAYLLRNQAMSEKRKVWEDMLSVMEKLEMPINE
                     KQRNFFLKA"
     gene            11888..13300
                     /locus_tag="VG754_11495"
     CDS             11888..13300
                     /locus_tag="VG754_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19887.1"
                     /translation="MKELNDSPILTTRREFLKTSGKIAAVSTLAGLAIPHVHAAPNNT
                     IQIALIGCGGRGGGAASNALSVTKLPIKLVAMADVFSAKLETAYNALKHAHGEKVDVP
                     AERKFIGFDGYKKAMDCLQPGDIAIFTTPLAFRWVHFTYAIEKGLNVFMEKPLTADGP
                     SSRRMLELAQKASAKNLKCAVGLMSRHHRPMQELHKRIEDGEIGDILLMRGYRMAAGP
                     LAACFVEKWPGTPSEVLWQISVFHSFIWASGGLFNDFYIHHIDHLCWMKNDYPIKAQA
                     LGGRHYKTTSEGAPLVDQNFDSYSVEYTFKDGAKMYIDGRNMYGASEFYYSHAHGSKG
                     MAVVAPEGDYGNNGQPASTHRGQLPDKDLIWKSKVAPHERDPYQNEWNDFVDAIMKDQ
                     PYNEVPYGVQSSVTSNLGRLAAHTGREWTYEEALNSKVEYSPNADKMTMDGPAPIVGD
                     KDGKYPVPQPGTIVDRDYQV"
     gene            13391..13909
                     /locus_tag="VG754_11500"
     CDS             13391..13909
                     /locus_tag="VG754_11500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWF19888.1"
                     /translation="MKTTLYAGRFLALIKEGHWEYAERVNATGAAIIVAVTDEQKLLL
                     VEQFRIPVHSRTIELPAGIIGDEPGAGDEPVAHAANRELIEETGYSAAQMELLMHGPA
                     SSGLTSETVSLFRATGLRRVGKGGGIEHEQITVHEVPLDKADGWLVDKAKTGVMIDPK
                     IYAGLYFLSKHP"
     gene            complement(13931..14686)
                     /locus_tag="VG754_11505"
     CDS             complement(13931..14686)
                     /locus_tag="VG754_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19889.1"
                     /translation="MTEAQTLANEAVQKLADRIRGGDRPPAHGMYYPDRPFREQVLHE
                     IRTDAGDLAAVVTRNSYGCQVLNTARVMFVDIDLPEPKSGGFFKMLFGKAGPPLVTQE
                     VAMAKIEKWSSQRPEWGWRIYRTRAGLRLMATHALVEPNSPDAEEVFEKLGADPLYRK
                     LCQTQKCFRARLTPKPWRCGIRSKPARWPWLDARQEKLFQKWDAQYQSFSFNWATCAL
                     LSQIGNPTVHPEVQPILKLHDGATRVESDLRLA"
     gene            complement(15003..16256)
                     /locus_tag="VG754_11510"
     CDS             complement(15003..16256)
                     /locus_tag="VG754_11510"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 71/99-like protein"
                     /protein_id="HWF19890.1"
                     /translation="MKLAKTILAALVLLLNLGACDSFASTNGKPVMVYYMPWYAAKPF
                     SAEWGWHWTMNHFDPDKVNASGEREIASWYYPLIGPYDSADPAVLEYHVLLMKLAGID
                     GVIVDWYGSADYFDYAAVNRNTARLFDYTRKAGLKFAICYEDQTIQRLIDGNRLTATE
                     AVHHAQQEMLYLQSKFFRDPSYFRLKDKPLLENFGPQYFLSSTNWAEIFSVLEASNRP
                     TFFTEDNLLAAGLGAFDWPPMWLSQQPGTGGVLSEASLKNYLVDFEKKSASWPAFISS
                     AFPRFHDIYQRAGVRNYWGYLGDRDGETLRENFNRALTNNSALVQIVTWNDFGEGTTI
                     EPTKEYGFRDLCMIQDLCRQKLDSKRPLRTNDLASAFQFYNLRRNSATNAALASALDQ
                     VFTGIMSGQEENALNQLHKLEAQFP"
     gene            complement(16280..18646)
                     /locus_tag="VG754_11515"
     CDS             complement(16280..18646)
                     /locus_tag="VG754_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HWF19891.1"
                     /translation="MICIVSAVSVKATSGASVPWMTYEAENMLVSGGTILGPQYNPIR
                     IPTEASGRMCVQLDSTGQYVQFTNQSAATALVVRYCVPDTSDGVGADYTLSLYTNGVL
                     AARLPVTSKYSWLYGNYPFSNNPSLGFARNFFDEVRTNGLILNPGTVVRLQKDATDTA
                     SYYVIDLVDVENVPAAPSQPANSLAVTNYSAVGNGVTDCTTDFQNCINAANSQSKSVW
                     IPPGDFLITGTINLYSNTKLLGAGMWYSRLVGSPSLYTTPSRRVNLNGSGSNINLSDF
                     AIVGCLNYRNDYEGNDGLGGSYGTGSSISRIWIEHTKSAAWIFNSSGLVVDSCRFRDT
                     LADGINVNYAMQNTIVTNCTTRGTGDDCFAIWPSPNSGSYTPGNNVITHCTGQLPFLA
                     NGGADYGGANNRIEDCLFSDLCYGAGILFSTTFNVSYTFSGTNVAQRCDIIRCGGYDG
                     GYGWRSALQIVIDNYGSNGIPGLNLNNLNIIDSVSTGFSILGGAGPLTNATATSVNIP
                     DYNLNNISGQHAWWAKCYNGCPIGSMTVSNSVVPEYQNDSGTFNFNFISNVVHVSVQP
                     SVAGAAFLVDGTTCTNTNTFTWPQGSNHTLAASNSQSAWTGTQYVWNSWSDGGAISHS
                     VSPTLSTNYTANFTTQYYLTLNSGLGGSVSPVSMWTNSGVSLSINATPSNGFAFSNWI
                     GTGNGCYSGGTNPAMITLNGPVVEQAAFLPPAPSITKVSVNSSNTVLLKYQTQPGFYY
                     HVETTTNLVPPSWAALSGSTTNASGTLATFTDTNQHTGGRRFYRAVSP"
     gene            complement(18899..21217)
                     /locus_tag="VG754_11520"
     CDS             complement(18899..21217)
                     /locus_tag="VG754_11520"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF19892.1"
                     /translation="MAASQNVITDPNLAYYGYENVFTNGLTATTQPAYISQYLGSGGG
                     GIGAAFPNDSSVDTLGNVTIGADDWPDVTTPYNTDTNIWTDATGTSAAICETLSDIYA
                     ENTSFSAGDSVTFTGSLATNTLASPYNGNAILFIKDYDSGWGFHGFVSLHINTLTNGQ
                     AFSLTMPSIFGNNDHIQYGLEWSGPPVRSGSAASYGMAMISTNGAVVAPPPPPKNVYV
                     YLDPSQQWAGYRNVVDQFGQNYGPASGYIGVGGQTPDFQGTISSQGLVRCAPDISLDV
                     YFQTDLNIWADASGTSSAVATYDNTFYVDSTGTGANGGDTVVFSGTLVTNALTDAGMS
                     GSLVAFIKDFSASWAYYGEQTVALSSLTNGQTFNMTKVINGTGDHVQWGFEWQGVPTR
                     TNASAANYVGQYGYVLFSSNSAVSSGPQILAITPSTANALLGSNVTFTANAIGNSLAY
                     KWSKNGVNLVNGGGISGATSSTLNLNGVQPSQEGTYTLVVTDSSSLSATNTVSLVVYN
                     PSWLYFDRALAPFNGYINVWNGGNLLTNPPASGAGGTTPPASFGFGVTPTSLVRATMN
                     LSNDTITLQPNTYVYDGATNTMDPNYINPDGSAAAYMEQDYYIQNDSLAGDVITFSGY
                     CPSNTLNAKYTARAWIKDGSPSWNVEHRYDAPLVAGQPFKVTVASTPGDHIQYGFGLW
                     GPDNSSTNPITQGAVVVKVYSPISSVNRSGSHFDLSFPTVINHSYSVQYKTNLTDSTW
                     TTLTTSNGTGINVTVTDTNAAASRRFYRLSTQ"
     gene            complement(21521..>22055)
                     /locus_tag="VG754_11525"
     CDS             complement(21521..>22055)
                     /locus_tag="VG754_11525"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19893.1"
                     /translation="IPQQHYAEAETLLRQTLAIRPIRTDGWIELGNALALQKKYTEAL
                     DCFATALEQDPGNGQIFLKRGKVLAHLNRHEEAIASYGAGLALDPSDGMIHHELALEL
                     VAVGQMDAAGEEFRKAAQLSQGSVAVRFDYGTWLLRQRQWPEAQREFQAVLQMEPGNT
                     RAQKHLAWLQAKLSDNH"
BASE COUNT         4965 a         6147 c         5914 g         5029 t
ORIGIN      
        1 aaccggcgca ttgaatcact tgctcctcct gcgacgtgca acactcgggt gcgaggctaa
       61 cagcagatga aatataaaat ggcaacagtc ctgatgctca gcatcctttc cggattgctg
      121 ctggccgggt ttgccgcgta cgccgacgaa tcagctcctt ccgaatacca ggtcaaggcg
      181 gcgtttcttt ataattttgc caagtttgtg aaatggcctg agtctgcgtt tgcccagacc
      241 aattctccca tcaagattgg catccttggt gaagatcctt tcgggggcaa cctcgatctg
      301 gcgatcaagg gcaagaccat caatgggcat ccgctcagcg tcatcgagtt gtcgtccccg
      361 gatgaaatga aaaatttcca gatcattttt gtctgccgga aacccaagcg caatctggtt
      421 gccacactcg cgcacctcaa caatgcgagt gtccttacgg tcagcgaaac cgacgatttc
      481 ctcaaagccg gcggcatgat caagttcgtc atggaaaacg ccagggtccg ttttgagatc
      541 aatgactcag cggcaacccg gtcgggcctt accatcagtt ccaagcttct aaacgtggcg
      601 cgaaaacggg agagcaacga ttagcggcat gaaagccttg agacacatct caatcaaccg
      661 gaagctcacc ttgatcatca tggtggcaag cacggttgcg ttgctgctca tttccgcttc
      721 attcgttact tatgaattga tcacctttcg ccagaggatg cgcaccgagc tttcgagcct
      781 ggcaacattc atcgccattc aaaacgatgc cgctctcctt tacggaaaaa atacagatgc
      841 ttacgacagt ctcggcgcat tgagcgcgaa gccgcacatt gtttctgcct gcgtctatga
      901 cgcccaaggg aaagtatttg ccacttatcc ggcgaacatc tcccataccc tccttcccaa
      961 acgtcccggc accgatgaat cctggtttga gaatgatcat ctgaaattgt ttcacagcat
     1021 cgtcaaggac gatgacatca tcggcaccgt ctatctggaa tctgacttga acgaattgca
     1081 ccagcggttg ctgcaatatg ggggcatcat cctgttgttc attttagggt cgctgctgat
     1141 cacgtttctg gtttcgtctt ttctgcggaa cattatctca gagccgattg cgcaccttgc
     1201 gcgaacggcg agagcagtat cggcggaaaa aaaatattcc gtgcgggcgg tgaagctcag
     1261 cgaggacgaa ctcgggcagt tcgtagatgt gttcaatgaa atgctttcgc aaatccaggt
     1321 gcgcgacgcg gcgctgcagc aggcaaacga cgaattggaa aagcgcgtcg aggaacgcac
     1381 gcgggacctg aagtcggaaa ttgccgaacg ccagcgcgcc caggatggtt tgcagcagca
     1441 actcggccgt atcagtctcc taaaccatat cgcgcaggtc atctcggacc ggcaagacct
     1501 cacgagcatt ctccatgtcg tgcttcgtca gttggaagat catcttccag tggatctggg
     1561 cggcgtttgg ctgctcaatc cgcagggcga cgccctgacc gttgcggctt tgcgtgaaaa
     1621 aaatcctttc ggtcccgtcc agccattgtt gaaaaacggg gaaatgctgc cgctcgaacc
     1681 cggcggactt tccagatgca aattgggcga aactctctat tgggcagata cgatggaact
     1741 ttcggcacaa ctggcacaaa ggctttcagg cgccggcctg cgttgcgccg tctcggtccc
     1801 gctgatggtg gagggaaagt tgtttgccat actctctgtt gcgcgccaga ccgcgaacag
     1861 cttcaccagc ggtgaatgtg aattcctgaa aatgttaagc gagcatgtcg cccttgccgc
     1921 tcatcaggcg aagctccaca ctgaattgga aagcgcctac aatgacctac ggcacagcca
     1981 gcaggcggtg atgcaacaag accgcttgcg cgcactcggg cagatggcca gcggtatcgc
     2041 gcatgacatc aacaacgcac tctcgccggt tgttggtttc gccggtctcc tcttggaata
     2101 tgagccaaat ctcagcgact cggcgcgcaa aaatttgacc tacatccgca cggcaggcga
     2161 agacgtggcc cacatcgtcg caaggatgcg cgagttttac cggcaacgcg acaagcagga
     2221 aatcctcttc ccgctcagtc tcaacgaact cgccgaacag gtgattgaca tgacgcgccc
     2281 gcgctggcgg gacatccccc aagggcgcgg catcatggtg gagacgcaga ccgatttcgc
     2341 caaggacatg ccgcaggtgg ctggcatcga agccgaagtg cgcgaagcgc tgacgaatct
     2401 catgcttaat gctgtggacg cgttgcccag cggcggcaaa ctcatcgtac gcaccagcac
     2461 gccttacctc cccacaaccg agcaggccaa aaacactccc aaacacggcg tccttgaagt
     2521 gattgacaac gggactggga tgagcgaact cacgctcaag cattgcctcg agccgttcta
     2581 ctccaccaag ggacagcgcg gcaccgggct tggactcgcc atggtttacg gcgtcatgga
     2641 acgccaccag ggcaagatcg agattgaaag cgaactcggc aaaggcacaa ccgttcgtct
     2701 catattccca ttgctggcag aacgccccgc agccgatacc aatggaaaaa ctattgcccc
     2761 gctgcgcccc ttgcgcatcc ttagcattga tgacgaaccg cttctgcgcg aactggtgaa
     2821 ggagatgttg gaaagggatg gccatcatgt gcaatccgtc gatggcgggc aggccggtgt
     2881 ggacatgttc cacaaagcga tggaaaaaca ccacccgttc gatgttgtgc tcacggattt
     2941 gggaatgcca ttcgtcgatg ggcgtgaagt cgcacggtta ttgaaaaagg cgtcgccgca
     3001 aacgcctgtc gtcctgctta ccggttgggg cgcattcatg aagcgcgatg gcgaggtgcc
     3061 cgcgcaggtg gacggtgtcc tgagcaaacc gccgcgtcta aatgagttgc gcgaaatgct
     3121 tgcgcacgtc acccaagggt aagtttaagg ttcgaagttc aaaattcaaa gttgtgatct
     3181 gctgtgcttc ggccaacttt aaaccttgaa cctgctgctt tgaacttgtt taccccacct
     3241 ccttggcgtc ttacaaattg tattgtgcac tggcaaatta tttgccgcgc tggcgcaaaa
     3301 ttttgcgcaa ctcgtccaaa tcctgcgaag acaacggctc cttctccatg aaatgcatca
     3361 gcaacggttt cactgaaccg ccgaacacgc gatgcaggaa cgacgcgctt tccgtccgca
     3421 cgcagtccgc ctccgaaacg agcggacgat atagataaag gttcttcact ttctccacgc
     3481 ccagagcctt ttttttatgc aaccggctca gcagcgtctt gaccgtattg ggatgccagt
     3541 cttccgtgtc ggcaagcgcg ttgaaaatct ccatcgccga aagcggtgaa ttggtccaaa
     3601 ggaccttcat cactttccat tccgcttccg atatgactgg aacttgcgcc atgaatcaaa
     3661 attgacgtta tcactttcgc gatgactgac ttctacctac aatagtagtc gcaaaaagtc
     3721 cactcttaaa ttttcaacct cttggtcaac attccctccc gctcaaatcg tcgtgaagct
     3781 gactgagacg ccagcacatg tcccctgcga tgatggcaaa caatccgcat tttaaatttt
     3841 taattcagca gcattccata atcggcgtcc agccagtcgg gacctgctat tttattggca
     3901 aatccagatg aacggcagaa aatgagatta agagttttta ttaggaaatc atgaaatcag
     3961 gaacaaaatt cttgaggaaa catcgtcttt tcctaatttc ccgctttcct aattctattt
     4021 tcccgcggct ctgttttttc ctttttaatt ttagatgcct tacctcggct ccggcgcgat
     4081 ctgcaacaac cccgcctggt cgagccgctg gatttcagcg gtgtaacctt tgcggaaatt
     4141 cggatatttg aattgataac caagttccat cttcagacgc cggttttgca ctttcttgtt
     4201 cgtcaagccg cgcttacgct ccgcgctctc ctcctccgtc gcgaatggcg gcatccattt
     4261 gcccaaggtc tcggaaagcc agcgaaaaaa atggatttgc gccaccggct cgtcatccac
     4321 ggcattgtaa atctctcctg cgcgcccgtt cttcagcgcc gcgacgatga ctccgaccaa
     4381 atcgtcgcga tggatcatgt tgatgatccg ttctccctgc ccggcaatct tcgcctcgtt
     4441 cttgagatat tgcagaaaca aatgtccacg ttccggtccg taaatgccgg ccacacgtaa
     4501 cacaactgct ggaagttttt tttgctgcgc cgcttccagc aatatttttt ccgtttccag
     4561 cagcacacgg ctcgtttcac tcgccggatc tgtggagctg gtttccttca ccgatgaacc
     4621 gtccgtttgc ccgtaaacac tggtgctgct ggtatagaca aactttttcg gcggcgctgc
     4681 cgcaagccat tcgataacat ttcgcgtccc ctgcaagtaa accgcccgat attcttccac
     4741 tccgccacgc gttgaagaaa cgcaattcac cacccaatca aacggccccg gcaacgcggc
     4801 caattccgcc ggcttggtca cgtcggcaat caacggtttg attcccgccg ctgttaattc
     4861 cgccgcgcct tcgctcgcgc gacgcacgcc aaacacctca tgtccctgcc gcgccaactc
     4921 cgcgcccaaa gccgttccca catagccgca accaatgatc agtgttcgca tcgaaaaagt
     4981 gtaagctccc gatttgaaat acccaagtac ccaatgagaa aggttgtttg catttcgccg
     5041 ctcgcttccc tactctcgtc cctcttaaaa attgaaatga ccgtcatgag cacccagcaa
     5101 cccagcactt cttccgcccc aataaaaact tttcaagcag acgccctgcc tgtccgcgtc
     5161 tatgccagcc agactgaatt gtcgcaggac gtggcgcgca tcgcgcaggc gcatttgcgc
     5221 gaagtcctcg ccgcgaaagg ctccgcagcg gtcattctcg ccaccggcaa ttcgcagatc
     5281 aaattcctcg aggaactcat ccgcatcggc ggcgtggagt ggtcggagat cacccttttt
     5341 cacatggacg aatacctcgg catttccgcc gagcacaagg ccagcttccg cctttacatg
     5401 cgcgaacgcg tcgtcagccg catcaagccg cgcgtgttcc attatctcga aggcgacgct
     5461 ttgttgccga tggacgaatg tgaccgttac acggcgttgc ttcgcgcgca gccgattgat
     5521 ctttgctgcc tgggcgttgg tgaaaacggc cacatcgctt tcaatgaccc gccggtcgcg
     5581 aattttgaag accctcgcgc cgtgaaaatc gcgaagctgg acgaagcctg ccggatgcaa
     5641 caagtcggcg aaggtcattt cgccaacatg ggcgctgttc ctcaatacgc actcacgctc
     5701 accatcccga tgctttgttc cgcgaagaaa atgctttgca tcgcccccga aaaacggaag
     5761 gcgcaggcga tcaaagacgc ccttcaaggc ccgatcagca ccgcctgtcc cgcttcgttc
     5821 cttcgccgcc aagcccactg tacgatgttt ctcgatcgtg attcgacgag cttgctttga
     5881 aaaactaacc aggcgggtgt ttgggtattt gggtatttca cgaaaacgac ccttgctgta
     5941 ggtttgcccc atgttgtcag agaaaccgtg gaattgggac cgggtcgttc gggtgttcat
     6001 cgccgtcctg ttcttcgttt ccgtgttctt cctggtgatg gcggcgacgc agcattacgc
     6061 gggcaagact aaattggttg aaggctcgct tgcctacgtg gtgctggggt ccctcggatt
     6121 tcacggctcg attttgttcg ggacagccat tattctcgct tcagaccatc tcaattgggg
     6181 caaggccttt ggtttttcat cccggccatt gacccgggca atgctgctcg ggttcgccgc
     6241 agcgcttgtt tttttgccgg tgggcatggt gttgcaagat gtttcgctcc gggcacttga
     6301 ttggttccat gttcccacgc cgccacagcc cgcagtggag caattcgaca aggcgacttc
     6361 ggtcgcaacc cgcgtttacc ttgctttttt tgcggtggtc ctcgccccgg ttgcggaaga
     6421 aatttttttc cgcggtattc tctacgccgc catcaaacaa ttcgggtttc cacgcctcgc
     6481 gttgtggggc agcgcgctga tttttgcgac catccatctg acgccggcaa tttttgtgcc
     6541 actactgctg ttgagtctca tcctcgtctg gctctatgaa aaaaccgaca atctccttgc
     6601 gcccatcacc gcccatgccg ttttcaacgg catcaatctt ctgttgatgt attacagcga
     6661 tgacctggcg cactcgatcc aacgtttttt tcctcatttt aaatgaacga atttgaattg
     6721 atcggccggc ttgccgcttc gctgccctcc aactcctctg tcgtgaaggg cgcgggcgat
     6781 gattgcgcgg tgctcgattt gggtttgccc gaacgcctcg tgctgttcaa gacggatgcc
     6841 gtcgttgaag gcattcattt caccgccgaa acgccgccgg aacaagttgg tcacaaagcc
     6901 ctcgcgcgct gtctcagcga tattgccgct atgtccggca cacccaccgc cgcgctcgtc
     6961 acgcttgctt tgccgcgcga tttcgacgcg gcccatgtgg aaaaacttta tgccggcatc
     7021 aatgctcttg cgcgccgcta tgatgtcgcg attgcaggcg gtgaaacgac cacgaatcca
     7081 gagcgcattt tcatttccat cgcgttgctc ggcacggtgc cgcgcaacga atgcttgtcg
     7141 cgttcaggcg cccgcgtggg ggacgcgttg tttgtcacgg gcgaactggg cggttccatt
     7201 tccggcaagc atttggattt cgagcctcgc ctcgtcgaag cgcgttggct cacggaacat
     7261 ttttccatcc atgcgatgat tgatttgagc gacgggctgg ccggcgattt gcggcatgtt
     7321 ttgcgcgcca gcaaagtcgg cgcggagctg ctcgccagcg cgattccaat cagccgcagc
     7381 gcgaaaattc agtccaaggc cgagactgcg accaagacgc cgttactcgc cgcgctcacc
     7441 gatggcgaag attttgaatt gctctttgcc gtcgcaagtt gtgatgccgt cccgctgctc
     7501 gatgcgtgga aacggcaatt tccaaaactg aaattaagtt gcatcggcaa aatcatcacg
     7561 ggcgaaggcg tgaccatccg cgacaagcgc ggcacccatt cttttgaatc tcatggctac
     7621 gtgcatttcc aatagtcccg aggcaacgct ggccttcggc gaagaatggg ggcgcgccgc
     7681 ggcaagtgga tgggtcatcg gcttgagcgg cgatttgggc gcgggcaaga cgcaattggc
     7741 aaagggcatc gcgcgaggtc tcggtttcgc cggtcgcgtt cattcgccga cattcacgct
     7801 cctcaatcaa tacgaaggcg gacgactgcc cctctttcat gtggatttat accggcttaa
     7861 aacatccgac caaatcatcg ccgccggcct tgaggaatat tttcatcccg atggcgtttc
     7921 catcatcgaa tggccggaaa gatggacaag cccgagattc aaggttcaac attccaaatg
     7981 tttttatcgt cgcgttttga ttgaagtcat cagcgaaacg gaacgccgca tcacctatga
     8041 agattttggc ggttgaattt tcctcggaag aacgcagcgt ggctgtcgcc acggatggca
     8101 acatccgcgg aagcgcgatg gagaaggcga cgcgcgccac gcacgccttc gcgctcatcg
     8161 aacaggcgct cacgcaagcg cgcatcgaac gcgaacaaat tgaatgcctt gcgatcggct
     8221 tgggacccgg ttcttacact ggcattcgtt cagccatcgc gcttgcccaa ggctggcaac
     8281 tcgcattgcc cgtcaaattg ctcggcatct ccaccgtcga atgcctcgcc gcccaggctc
     8341 aaagtcaggg ctggtttggg aaggtgaaca ttgtgattga tgcccaacgt gatgaacttt
     8401 acttcgcgcg atacgaaatc tcttcgcacg gctatcgcga aattgcctcc ttgcgattgg
     8461 caactccgga cgaagtgcgc ttgcaatgta aatcagacga aatcatagcc ggtcccgacg
     8521 cgaaccgctg gttcgaaaaa ggccgtatta tttttccaga tgcggcgacg ctggcccgct
     8581 tggctgcgag ccggacagat ttcatgcccg gcgaaaaatt ggaaccaatt tacctgcgcg
     8641 aaaccaattt cgtcaaggca ccgccgccgc gaatttttgg ttgatcagcc gcggtgatgc
     8701 cattctccgt ggtgccactc gtaacgaccg cgttccgggt gccatcgcca gccgccttcg
     8761 atccagatcg cgcccggata aggcgccggg ccataatact cgaccactgg cgacggagga
     8821 ggtggaggtc ccggggggcc ggcagcaaca acacatccat tttctaataa caaagtgagc
     8881 gccagcatcc caactggcag gatcaaactg cgtttcataa cattcttcct acatcctcaa
     8941 ggccccactg gctggcaggt tgaattaatt tagcccatgc cgcagctcgt cgcaaaccat
     9001 cgaatggggt ctgcgccccg tgcttcaaac attcggtttg gcggtcggcg tgtttgctgt
     9061 cataaaatct gcacatgaaa gctgcggtcc tttatggtca ggaacagatt cgggtggaag
     9121 aagtggcgcc ccaaccgctt ccacccggca cggtgcgcat tcaaatcgaa gcggcgctga
     9181 cctgcggcac ggacctgaaa gtcttcaaac gcggctacca cgcccgcatg atcgttccgc
     9241 ccgcggtctt cggccacgaa ctttcgggca ccatcagcga agtgcatccc gaagcggctg
     9301 gttggaaaac cggcgagcgc gtggtcgttg cgaactccgc tccttgcggc aaatgttttt
     9361 attgcggcaa ccagcaggaa aatctttgcg atgatttgct ttttttaaat ggcgcttacg
     9421 cggaatccat cgttgtgccc gcgcggcttg tgcaaaaaaa tttgttgcgg cttaaaccgg
     9481 aaaccgcctt tgccgacgcc gcgcttgtcg aaccgctcgc ctgcgtcgtc cagggagtgc
     9541 aggaagcata cctgcgctcc ggccaaagcg tgctcgtcat cggcacggga ccgatcggcc
     9601 tgatgttcat cgcgctggcg caggacatgg ggtgcaaagt gaccgcggca ggacgcggtg
     9661 aagtgcgttt gcaaaccgcg cggcgtttag gcgcgaactg cgtcatcgac atcgccaatc
     9721 aaaatgattt ggcacgcacg atccaagctg gagcgaagga gccgtttgat gtcgtcatcg
     9781 aagccgtggg caagccggaa gtttgggaag ccgcaacgcg gctcgtgcgc aaaggcggca
     9841 aagcgaattt cttcggcggt tgtccttcag gcaccacggt cgcgctcgat accgcattgc
     9901 tgcattattc aaacctcaca ttgttggcga gttttcatca tacaccacgc gccattcgcc
     9961 gtgcgctgga attgattgag aagggcatca ttcgcgcgcg cgattttgtg gatggcgaat
    10021 gtccgttgac gcagttgccg caattgttca agagcatggc caatggcaac cgcgcggtga
    10081 aaacgctcat ccgggtccgg gaataatctt ggacgttcag cgttcgattt gattgttcga
    10141 agtttgtgat tctattttcc cggcgctgct ttcatgtgcc ggtgaacgaa aaagtaataa
    10201 atacttccca acaccaccat cgcgccgccc acccagatga cgatgcgatg atactcgcct
    10261 ttcatcaatt gctcgtcctg tcccatcttg atgccgagcc agcagagaat cgcgcaccag
    10321 atgccggaac cgagcacggt gaacaaggaa aataatctgt aatccatccg cacaatcccc
    10381 gccggaatgc cgatcaaatg ccgcaccacc ggcagcaagc gcgagatgaa aatgcccatc
    10441 gaaccaaact tcgccgacca gcgctccgct ccttcaattt tttccggcga aatcccgaca
    10501 tatcgcccgt atgccgcaac caatggcctg cccgcggtac gcgacaccca atacatgatg
    10561 gatgctccaa tccacgaacc caacgtgccc gcgataatga tgccgacaac gctaaatttc
    10621 ccggaagcgg ccgcgggtgg gattattact tcgcttggca gcgggaaaat ggtgctttcc
    10681 gccgccatga gcaaagccag ccgcgagtag ctcagatgat caagatacca gcggacgatc
    10741 ggttctaaaa aatgtttcat cgcgcagagt gcttaccggg aaaacgatgc aagggcaagc
    10801 ggcattcatg aatcagcgaa gaggcaaaaa agaattcatg cccaattaag aattggttca
    10861 tgctttcagg aaaaaattcc gctgcttttc gttgatgggc atctccagct tttccatcac
    10921 cgaaagcatg tcttcccaca ccttccgttt ttcgctcatg gcttggttgc gcagcagata
    10981 tgccggatga tacgttggca tcagcgggat gttgcgatag gtctgccatt gcccccgcag
    11041 ccgggtgatg ccaatggtct tgccgagcaa tccttcaacg gccgtcgcgc ccaacgcgac
    11101 aagcaccttc ggcttgatca agtcgatctg ctcgtgcaaa aacggaatgc aggtttccat
    11161 ctcctgcgaa gtcggcttgc gattgcccgc tgtctggcct ggggtgtcgg gacggcattt
    11221 caaaatgttg gcgatgtaaa catcctcgcg cttgaggccg gttgcctgga tgattttggt
    11281 gagcaattgc cccgccttgc cgacgaatgg ttcaccttgt tcatcttcat ccgcacccgg
    11341 cgcttcgccc acgaatatca gcggcgactc gatattcccc acgccgaaca cgacgttttt
    11401 gcgcgatgat gcaagatgag agcacttcac gcacgccatc gcccgtgaac gcaactccgc
    11461 aaatgcagcg accttcgccg ggtccgaagt tgtcgccaca gaaaaaatcg gagccacagc
    11521 cgggcttatc gtatgcggtc gaattgtcga tacagcggga gcagttgacg aagttggaac
    11581 cgtcctgcgc gccgtcggtt ggcccaaaac cgaaagattt tctggcgaca cagacacaaa
    11641 atgcacaccc tgctctttca aggtttccag atgttgaatc gtggcatcga gcagttgaag
    11701 gtaggcatcg gacatcgtgg gcagcttaat atagagggcg ggaaaagcac ggaaaaatta
    11761 ttcccaaccg catgaacgtg cggttgttca atgcctgatt tcgcgtcttt tcttcttggc
    11821 ggcacggcgc tggttgttaa taatccgttg aattgcccat gcagcagcaa cgtcaatatc
    11881 agcatctatg aaagaattga atgactctcc gatcctcacc acacgccgcg aatttttgaa
    11941 gacttccggt aaaattgccg ccgtttccac actggctgga cttgccatcc cgcatgtcca
    12001 tgccgcgcca aataacacca tccaaatcgc ccttatcggc tgcggcggcc gtggcggcgg
    12061 cgcggccagc aacgccctga gcgtcaccaa gctgcccatc aagttggtcg ccatggccga
    12121 tgtcttttcc gccaagctcg aaaccgccta taacgcgcta aaacacgcac acggcgaaaa
    12181 agtggacgtt cccgccgagc gcaagttcat cggttttgac ggttacaaaa aagccatgga
    12241 ttgcctccaa ccgggcgaca tcgcgatttt cactacgccg ctggcgttcc gctgggtgca
    12301 tttcacctat gccatcgaaa aaggtttgaa tgtcttcatg gaaaaaccgc tgacggcgga
    12361 cggtccctct tcacgtcgca tgttggagct tgcgcagaaa gcctcggcaa aaaatttgaa
    12421 gtgtgccgtc gggttgatgt cgcgccacca ccgtccgatg caggaattgc acaagcgcat
    12481 cgaggacggc gaaatcggcg acattctttt gatgcgcggt tatcgcatgg cggcgggacc
    12541 gcttgcggcc tgtttcgtcg agaaatggcc cggcactccc agcgaagtgc tctggcaaat
    12601 ttcggtgttt cacagcttca tctgggcgag cggcggtctc ttcaacgatt tttacatcca
    12661 ccacattgac catctttgct ggatgaaaaa tgattacccg atcaaggcgc aggccctcgg
    12721 aggacgccat tacaaaacca caagtgaagg cgcgccgctc gtggaccaga acttcgattc
    12781 gtattcagtg gagtacacct tcaaagatgg cgccaagatg tacattgacg gtcgcaacat
    12841 gtatggcgcc tcggaatttt attacagcca tgcgcacggc agcaaaggca tggcagtcgt
    12901 cgcgccggaa ggtgattacg gcaacaacgg acaaccggcg agcacccatc gcggccagct
    12961 cccggacaag gacctcatct ggaagtcaaa agtcgctcct cacgaacgcg atccttacca
    13021 gaacgaatgg aacgacttcg tggacgcaat catgaaagac cagccttaca acgaagttcc
    13081 ctatggtgtc caatccagcg tcaccagcaa tcttggccgt ctcgccgcgc acactggccg
    13141 cgaatggacc tatgaggaag cgctcaactc caaagttgaa tactcgccca atgcggacaa
    13201 gatgacgatg gacggccccg cgccgattgt cggtgacaag gatggcaagt atccggtgcc
    13261 gcaaccgggt accatcgttg accgcgatta ccaagtctga tcgccctttt ctccaatgcc
    13321 ccggcgcctt caaaacgccg gggcattttt ctttgtggat tggtgactcg ctgcaaaaga
    13381 aatctgacac atgaaaacaa ctctctatgc cggacgcttt ctcgcgttga tcaaggaagg
    13441 ccattgggaa tatgccgagc gggtgaatgc gaccggcgcg gcaatcatcg tggctgtgac
    13501 cgacgaacaa aaattgcttc tcgtcgaaca atttcgcatt ccggtccatt cgcgcaccat
    13561 tgaattgccc gctggaatca tcggtgatga gccgggcgcg ggtgatgagc cagttgcgca
    13621 cgccgcgaac cgggaattga ttgaagaaac cggttacagc gccgcccaaa tggaattact
    13681 tatgcatggc ccggcttcaa gcggtctgac gtccgaaacg gtcagtcttt ttcgcgcgac
    13741 cggattgcgg cgtgttggaa aaggtggcgg aatcgaacac gaacaaatca ccgtccatga
    13801 agttcccttg gataaagcgg acggctggct cgtggacaag gcgaaaacag gggtgatgat
    13861 agatccaaaa atctatgccg gcctttattt cttgagcaag catccttgag tccctcactc
    13921 cgatttgttt tcacgccaac cgcaaatccg attccacgcg tgtcgcgccg tcgtgaagtt
    13981 tcaatatcgg ttgcacttcc gggtggacag tcggattccc gatctggctc aacaatgcgc
    14041 atgtggccca gttgaacgag aaggactggt attgcgcgtc ccatttctga aaaagttttt
    14101 cctggcgcgc atccagccac ggccagcgcg cgggtttgct gcgaatgccg caccgccacg
    14161 gcttgggcgt caggcgggcg cgaaaacatt tttgcgtctg gcaaagcttg cgataaagcg
    14221 ggtccgcgcc gagcttttcg aaaacttctt ctgcgtccgg tgaatttggt tcaaccaagg
    14281 cgtgcgtggc catcaaacgc aatcccgcgc gcgtccggta aatccgccag ccccattccg
    14341 gcctctggct gctccatttc tctatttttg ccatcgcgac ttcctgagtg acaagcggcg
    14401 gccctgcttt tccgaaaagc attttgaaaa atccgcccga cttgggttcc ggcaggtcga
    14461 tgtccacgaa catcacgcgc gcggtgttca gcacctggca gccgtaggaa ttgcgcgtga
    14521 ccaccgcggc gaggtcgccc gcatcagtcc tgatttcatg aagcacctgt tcgcgaaaag
    14581 ggcggtcggg ataatacatc ccgtgggcgg gcggacggtc gccgccgcga atccggtctg
    14641 cgagtttttg gacagcctcg tttgcgagcg tctgggcttc ggtcatgctc tccgaagacc
    14701 agcgccagca aataaaatcc ccgatgttcc cacgtgccca aaatttcggg aaatgcatac
    14761 cggcacgata gaaacttgcc cggcagcttt ccagcaattt cgtaatgacg cagtaactgg
    14821 cggcatgact gaagccgact tctttgaatt caccgagtta cttttgcgct ttttttccgc
    14881 atgacgtcac gatggtgact tgagaacgct tctcgaagat gcttgaatgg gaatttaatg
    14941 atcatggtgc cagtgaccat gtccaaccag ttcttccagc tccaatcaca ctgacgccgc
    15001 atctacggaa actgggcttc cagtttatgc aattggttca atgcgttctc ttcctggccc
    15061 gacataattc ccgtgaagac ttgatccaag gcggaagcca gtgcggcatt ggtcgcggag
    15121 ttgcggcgca ggttgtaaaa ctgaaaggct gatgccagat cattggtacg caacggacgc
    15181 ttggagtcaa gcttctgccg gcataaatcc tggatcatgc atagatcgcg aaacccatat
    15241 tctttggtag gctcgatcgt cgtgccctcg ccgaaatcgt tccacgtgac aatttgaacg
    15301 agcgcggagt tgttggtcaa ggcgcggttg aagttctcac gcaatgtttc gccgtcccgg
    15361 tcgccaaggt agccccagta attccgcacg ccggcgcgct ggtaaatgtc gtggaaacgc
    15421 ggaaacgcgc tgctgatgaa tgccggccag gacgcggatt ttttttcgaa atcaacgagg
    15481 taatttttaa gcgaagcctc cgacaaaacc ccgccggtgc ccggctgctg gctcaaccac
    15541 atgggcggcc agtcgaaagc gcccagtccg gctgccaaca ggttgtcttc ggtgaaaaac
    15601 gtcggccgat tggatgcttc gagcaccgaa aaaatttcag cccaattggt gctggacaaa
    15661 aaatattgcg gaccaaagtt ttccaaaagc ggtttgtctt tgaggcggaa atagctgggg
    15721 tcgcgaaaaa atttggattg aagatagagc atttcctgct gcgcgtgatg gacagcttcg
    15781 gttgcggtga gacgattgcc gtcaatgagc cgctggatgg tctggtcctc atagcaaatc
    15841 gcgaatttca atccggcctt gcgggtgtaa tcaaaaagcc gggcggtgtt gcggttgaca
    15901 gcggcgtaat cgaaataatc ggccgagccg taccagtcca caatcacgcc atcaatgccc
    15961 gcgagcttca tcaacagaac gtggtattcg agaacggccg ggtcggcgga atcgtaaggc
    16021 ccgatgagcg ggtaatacca cgaggcgatt tcacgttcac cggatgcgtt caccttgtcg
    16081 ggatcgaaat gattcatggt ccagtgccag ccccactcgg cgctgaaggg tttggcggca
    16141 taccatggca tgtaataaac catcaccggt ttgccattgg tggaagcgaa cgaatcacat
    16201 gcgccgaggt ttagcaacaa tacaagggcc gcgagaatgg tttttgccag cttcatgttc
    16261 acaaaattat ttgggcacat tagggcgata cggcgcgata aaatcggcgc ccgcctgtat
    16321 gttggtttgt gtcggtgaat gttgccaaag tcccggaggc gttggtcgtg ctgccggaca
    16381 aggcagccca cgatggaggg acaaggttgg tggtggtttc gacatgataa tagaaaccgg
    16441 gctgcgtttg atatttgagc aaaacggtgt tgctgctatt caccgatacc ttggttattg
    16501 aaggcgctgg cgggaggaaa gccgcttgtt cgacgacggg cccgttcaag gtgatcatgg
    16561 caggattggt tcccccggaa tagcatccat tgcctgtgcc gatccaattg ctaaacgcaa
    16621 acccgttgga aggcgtggcg ttgatgctca gtgaaacgcc actattcgtc cacatactga
    16681 ccgggctgac actgccacca aggccactgt tcagggtcag ataatattgg gtcgtgaaat
    16741 tcgcagtgta attcgtgctt aaggtcggac taacagaatg ggaaatcgcg ccaccatcac
    16801 tccatgaatt ccagacatac tgcgtgccag tccaggcgct ttgcgaattg gatgcagcaa
    16861 gcgtgtgatt cgagccctgc ggccaagtga aagtattggt gtttgtgcag gtcgtgccgt
    16921 ccaccaggaa cgcggcacct gccacactgg gctgcaccga tacgtgaact acgttggata
    16981 tgaaattaaa attgaatgtg ccggaatcat tttgatattc gggcaccacg gaattgctga
    17041 cggtcatgct gccgatggga caaccgttgt aacacttggc ccaccaggca tgctggcctg
    17101 aaatgttgtt aagattgtaa tccggaatgt tcacgcttgt cgcggtggcg ttggtcaagg
    17161 ggccggcgcc cccgagaatg ctgaagccgg tggagacact gtcaatgata ttaagattat
    17221 tgaggttgag tccgggaatg ccgttggaac cgtagttgtc gatgacgatt tggagtgcgg
    17281 accgccaacc gtaaccacca tcgtagccac cgcaccggat aatatcgcac cgctgcgcga
    17341 cgttggtgcc gctgaacgta taggagacat tgaacgtggt gctgaacagg atgccagccc
    17401 cgtagcacaa atccgaaaac aggcagtcct cgatgcggtt gttcgcgccc ccataatccg
    17461 cgccgccatt ggcgagaaaa ggcaactgtc ccgtgcaatg ggtgatgacg ttgttgccgg
    17521 gggtatagct gcctgaattc ggagacggcc agatggcaaa gcagtcgtcg ccagtgccgc
    17581 gagtggtgca gttggtcacg atggtatttt gcatggcata attcacattg atgccgtccg
    17641 cgagcgtgtc acggaaccgg caactatcca ccacaaggcc gcttgagttg aatatccatg
    17701 cggcagactt ggtgtgttca atccaaatgc gcgagatgct ggaacccgtg ccataacttc
    17761 ctcccaggcc gtcattgccc tcgtagtcat ttcgatagtt caggcagccg acaatggcga
    17821 aatcggacag gttgatgttg ctgcctgaac cgttcagatt aacgcggcgg gatggagtcg
    17881 tgtagagtga agggctgccg accagtctgg aataccacat gcccgctcca aggagtttcg
    17941 tgttggaata cagattgata gtgccggtga tcaaaaagtc gccgggagga atccagacgg
    18001 atttgctttg ggagttcgcg gcgttgatgc aattctggaa atcagtggtg caatcggtga
    18061 caccattgcc gacggcactg taattggtca ccgcaaggga attggccggt tgtgagggag
    18121 cggccggaac gttttccaca tcgacaagat caatgacgta ataggatgcg gtgtcagtgg
    18181 catctttttg caaacggacg acagttccgg gattcagaat caacccgttg gtgcgcactt
    18241 cgtcaaaaaa gtttcgcgcg aaaccgaggg agggattgtt cgaaaagggg taattcccat
    18301 atagccagga atatttcgag gtcacaggca gtctggctgc gagcactccg tttgtgtaaa
    18361 gactgagtgt gtaatccgca cccacaccat cggatgtgtc cggcacacag tagcgcacta
    18421 caagggccgt ggcagcggat tggttggtga actggacata ctggcctgtg ctgtccaact
    18481 gcacgcacat gcgtcccgac gcctcggtcg gaattctaat cgggttatat tgcggcccca
    18541 agatcgtgcc cccgcttacc aacatgtttt ccgcctcgta agtcatccac ggcaccgacg
    18601 cgccggatgt cgctttgact gaaaccgccg aaacgatgca aatcaaagaa gccagaatga
    18661 tacaaatcgc aatctttgaa catccagttc caattcggcc cccattgtgc ggctggatga
    18721 atttacaaat cataattgac gatgggctgg aatctttttt tgcacgaacc gagatttctt
    18781 ttggttcttc caccgcgcca gagccagcca tattacttat gctattccta aagcaatccg
    18841 tcgagcctga aaccccacaa gctcgacgga cgctcgatta caattcgacc aacagttttt
    18901 attgagtgga tagccgatag aagcgacgcg aagcagcggc gtttgtatcc gtcacggtaa
    18961 cgttgattcc cgtgccgttg ctggtggtca acgtggtcca agtgctgtcc gtcagattcg
    19021 ttttgtattg cacggagtag ctatgattga tcaccgtcgg gaagctcagg tcgaaatggg
    19081 aaccggagcg gttgaccgaa ctgatcggcg aatagacctt gaccaccact gcgccttgcg
    19141 tgatggggtt agtgctggag ttgtcaggac cccacaaacc aaatccatac tgaatgtgat
    19201 cgcccggagt tgacgcgacc gttaccttga atggctggcc ggccacgagc ggggcatcat
    19261 aacgatgttc cacattccaa ctgggtgagc cgtctttgat ccaagcccgg gcggtatatt
    19321 ttgcgttcag ggtgttgctc gggcaataac ccgagaatgt gatgacatct cccgccaggc
    19381 tgtcgttctg gatgtaatag tcctgctcca tgtatgccgc tgccgatccg tcaggattga
    19441 tgtaatttgg gtccattgta ttggtggcgc catcataaac ataagtgttc ggttggagcg
    19501 tgatggtatc gttggataag ttcatggtgg cccgcaccaa ggaggttggc gtgacgccaa
    19561 aaccaaagct ggccgggggc gttgtgccac ccgcaccact ggccggtgga ttggtgagaa
    19621 gattcccgcc attccaaaca ttgatgtagc cgttaaatgg cgccaaggcg cggtcgaaat
    19681 agagccacga cggattgtac accacaagtg aaaccgtgtt cgtggccgaa agggaactcg
    19741 agtcagtgac gaccaacgta taggttcctt cctgcgatgg ttgcacaccg ttcagattca
    19801 acgtgctgct ggtagcgccg gagatgccgc caccattgac taagttgacc ccatttttcg
    19861 accatttata ggccaaacta tttccgatcg cgttcgccgt aaaagtcaca ttagagccaa
    19921 gaagcgcatt ggcggtgctg ggtgtgatgg caaggatctg tgggccagag gaaaccgcac
    19981 tgttgctcga aaacagaaca taaccatatt ggcccacata gttggcggct gacgcattgg
    20041 tgcgcgtggg cacaccctgc cactcaaaac cccattgaac gtggtcccca gtgccgttga
    20101 tcaccttggt catgttaaat gtttggccat tggtcaggct gctcagcgcg accgtctgtt
    20161 ctccgtaata agcccaacta gcactgaaat ctttaatgaa ggccaccaag gagccggaca
    20221 tgcccgcatc cgtcaaggcg ttggtcacca aggtgccgga aaacaccacc gtgtcaccgc
    20281 cattggcccc ggtgcctgtg ctgtcaacat agaaagtatt gtcataagtt gccaccgcac
    20341 tgctggtgcc ggacgcatcc gcccaaatat tgagatcggt ctgaaaatag acatccagac
    20401 tgatatcagg cgcgcatcgc accaaacctt gactgctgat ggtcccttgg aagtccgggg
    20461 tctgtccgcc gacacctatg tagccgcttg cggggccata attttggcca aattgatcca
    20521 ccacattccg gtagcccgcc cattgctggg atggatcaag ataaacataa acgtttttcg
    20581 gcggcggcgg cggcgccacc actgcgccat tggtgctgat catggccatg ccgtagctgg
    20641 cggcgctccc ggaacgcacc gggggtccgg accattccaa cccgtattgg atatggtcat
    20701 tgttcccgaa aatcgaaggc atggtcagtg aaaaagcctg gccgttggtg agcgtgttga
    20761 tgtgtagcga gacaaagccg tgaaaacccc agcccgagtc gtaatccttg atgaagagga
    20821 tcgcattgcc attgtagggt gacgccaaag tgttcgttgc aagggagcct gtgaaagtca
    20881 cagagtcgcc ggcgctaaag ctggtatttt cagcgtaaat atcgctgagt gtttcgcaaa
    20941 tggcagcact cgttccagtt gcgtcggtcc agatgttggt gtcggtatta tagggagtcg
    21001 ttacgtcggg ccaatcatca gctccgatgg tgacgttacc caaggtatcg acggaggaat
    21061 cattgggaaa ggcagcgcca ataccaccgc caccagaacc caggtattgg gagatataag
    21121 ccggctgggt ggtggcagta agtccgttgg taaaaacgtt ctcatatccg taatacgcaa
    21181 gatttggatc ggtgatgaca ttctggctgg cagccatggc gccaaaagtt ccaagccccg
    21241 cgaccagagc cgacacaccg aaggcaaata tgctccttgc tggtttactg actattttta
    21301 ttccggttct tctcataact tgaggtggat gctttttgga cgaatcgtcg tttcgacact
    21361 gttcgtcgct gttttgtttc attgggacta tgcatgtata tcagtttttc acttacccgc
    21421 acgtcgcatg aagtggtgac aactgcccgg ctggaatctg taatgaactg gggaaattcg
    21481 tcgaaaaatt taaactcagc ttgaaacgga ggattgccgg tcaatgattg tcggagagct
    21541 ttgcctgcaa ccacgcgagg tgtttctgtg cgcgggtatt tcccggttcc atttgcagga
    21601 ctgcctggaa ttcacgttgc gcctccggcc attgccgctg tctgagcaac cacgtcccgt
    21661 aatcgaaccg cactgcgacg ctcccctggc ttaactgcgc ggccttgcgg aattcctcac
    21721 ctgcggcgtc catttgcccc accgcgacca actccaaggc cagttcatga tggatcattc
    21781 catccgaagg gtccaatgcc aggccggctc cataactcgc aatggcctcc tcatgccggt
    21841 tcagatgggc cagcactttg ccgcgtttta aaaaaatttg cccgttgcca gggtcctgtt
    21901 ccagcgccgt ggcaaaacaa tccagcgcct cggtgtattt tttctgcaac gccagagcgt
    21961 tgcccagttc gatccaacca tccgtgcgga tgggccggat ggccaaagtt tgccgcaaca
    22021 atgtttcggc ttccgcgtaa tgctgctggg ggatc
//
LOCUS       DASZMM010000478         9821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4605654, whole genome shotgun sequence.
ACCESSION   DASZMM010000478 DASZMM010000000
VERSION     DASZMM010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4605654"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VG754_11530"
     CDS             <1..238
                     /locus_tag="VG754_11530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWF19894.1"
                     /translation="TDAFPVLQQHGFSATMFLPTAFIREKRRMFKERECMTWNEAREL
                     HRAGIEFGSTPSIIPRFMNWISATFAPSSRFPNG"
     gene            235..567
                     /locus_tag="VG754_11535"
     CDS             235..567
                     /locus_tag="VG754_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19895.1"
                     /translation="MIEDKLGVAISSFAYPYAFPSADRDFVETLTDLLKGSGYECNAT
                     TRIGRVHAEDDPYMLKRLPVNSADDSALFLAKIRGAYDWMNWPQDRLKKLKVAANGRT
                     QQRLCRTP"
     gene            579..1460
                     /locus_tag="VG754_11540"
     CDS             579..1460
                     /locus_tag="VG754_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF19896.1"
                     /translation="MMRTKTCPNYVLVTPVKDEEALIGKTVESVVNQTVPPSEWVIVS
                     DGSTDRTDAIVREAGVMHPWIRLVQLPVRQQRSFAAVVRAVEKGVASLQKKDYQYIGL
                     LDSDLRFKRDYFEKVIEHFECNVQLGLAGGVVIDVDSPEQRLPRNRQDVPGAVQFFRR
                     QCFEGLDGLLGIPEGGWDALTCARARMIGFETRLLTELIVDHLKPRNVSEGGLLRRQW
                     QMGIRDYALGYHPLFELFKCLARMGQKPFFIGCIAWWMGYCSAAIQRRERFIPKELLN
                     FVRGEQKRRLVRALIGS"
     gene            1872..2564
                     /locus_tag="VG754_11545"
     CDS             1872..2564
                     /locus_tag="VG754_11545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWF19897.1"
                     /translation="MTHRLKPGDVFIDVGANVGYFSCLASKLVGDSGKVVSIEALPQI
                     FERLKENLAMNGAHNVRLANVAVWNKEENLKMFTRMAHPLGATSLMPAWAAHWQLETA
                     VEVPAKPLPAILTQEEITRARLIKIDVEGAEWKVISGMADMLPDSRQDLEIIVEISRG
                     MLKEQGKSAGDLLELFARHGFHPYRLINDYSVGAYVAPRIPQPPKRVETILDSAEDQM
                     DVIFSRAGGASL"
     gene            2621..3862
                     /locus_tag="VG754_11550"
     CDS             2621..3862
                     /locus_tag="VG754_11550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF19898.1"
                     /translation="MNEKSKRIRVLYSFPLKIGAGRVCTIAWYQAQGLANAGAEVTVF
                     PASICRDFTSAVKVQPTLAWGKFRLPNRLVGRRAYAIMHDRIVARRLEKIADRIDIIH
                     TWPLGARETLKAAAKLGIPTVFERCNAHTRFAYDVVNKECERIGVPLPPGHEHAWNTE
                     LLEREEAEYQLAYRLLCPSEFVVKTFVDHGFAREKLVRHIYGFDQKIFYADNKPRDPK
                     RGLTMISVGVCAVRKGLHFALEAWLKSPASRDGTFLIVGEFLPAYREKLASMLAHPSV
                     KVLGHRNDVPELVRQSDVLVLPSVEEGFGLVVTEAMASGCVPLISDACTDLCKHMENG
                     MVHRVGDMGMLERHIATLHEDRGLLEKLRAAGLRLAPEITWNAAGVKLLDAYREVLAA
                     AGTTRSHRCVGDKDESERVKA"
     gene            3859..5190
                     /locus_tag="VG754_11555"
     CDS             3859..5190
                     /locus_tag="VG754_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19899.1"
                     /translation="MNAAGATICSILVLVVLIAPRRWALLGMIAGALFLTERQQVSVG
                     GINFYSIRFLELAGFVRVMSRAEFSFRSCNRIDRALIFLYVYTAVVFCLRSAEGQAYI
                     IGNSVDAFLCYFTFRGLVRDASELKLFLRDFIMLLLPYAAMILYESMTHRNAFAFIDG
                     GMYNWVRGNRFRSVGTFRNPDTLGTLGAVFAPLYFAIAFAQRERKLPIFGMLLCLCIV
                     WGSNSGGPLCATAVSFVAWGFWRMRTRMQTVRRTMAAILILLACVMKAPIWFLLARAS
                     SVAGGDGWHRAQLISVAFKHLNLWWFAGMPLSDTSEWFEYSIGGAADITNTFLSFGLN
                     AGMGAIIVLVLLLVRTFSGLGKALNSVRSNGQEDDYREAMLWGLGAALMAHIANWQGI
                     VYFDQSYMVWFLQLAAMTSVSSACIEADEAETVRVSPVEEVHASRGEEVRI"
     gene            5202..6104
                     /locus_tag="VG754_11560"
     CDS             5202..6104
                     /locus_tag="VG754_11560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWF19900.1"
                     /translation="MLVSVIIPTYNRARTIERAINSVLSQTWTPIEIIVVDSWSTDRT
                     VELLAQYGDKVRLISQEKMGPGFARNAGIKAAKGEIISFLDSDDEWLPEKTARQVKLL
                     QASGVNCCVCNARMEFISGAVNSFDVSRLRSKLREGIWTNPTEVLVTRFLFFNQVVAL
                     RREALERTGYFRQGIMEDYDLALRLSLLGPWAFIADPLVVWHEQAGDNLSKTHSQLEI
                     CQRTLEILEETSSSSQWGSLLPEKLTRSRMKFLSKRVEALRLSALSNPLARLSGKFRL
                     QLLHTGLKLYWRLPSTPRMNTRAV"
     gene            6370..8097
                     /locus_tag="VG754_11565"
     CDS             6370..8097
                     /locus_tag="VG754_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWF19901.1"
                     /translation="MKKAFVTAASFTFCWHLGAASYFVDYAGGADSNSGTATNAAWKH
                     CPGDVVATANPASTVLMPGDTVFFKGGVTYVIIPQTAGGPGIYLNWAGAAGNPINYDG
                     NSGGTWGSGKALMTDNYKGSVAFYSPAGMTSLVFKNFAIGPMGGAAVLPSDPKGTNYA
                     PNGIPPNPGGGFVCGGMINSVVISSCTFSNCGYYGSTYPLGNASLEGGALSCNGCNGF
                     TITNCTVSRMRDGIYLSNPSQISNLTIAGCTFNQIEEWGITMNTGVNAYLSNVYIFNC
                     LFTNVDQNYAAWGGYGSPPHRDTIFSFGAEGCNGSCSLQRNVTDTNVCIYNNYFVDTM
                     GYVGGTAVVWFTDNASANIYNNVFVNTGEANAAVKLNGPSDNSQWRAAVYNNTFYMQD
                     YEIPISFGSCPVNNSSWPISPTNHNPMITILNNNVYSWSGATPYVNGCNVLIEDSTPN
                     LTNNVVLDYNLYRSSLAVNSQMVYAQWPLYGGYIVQNVAQILYGWDVHSIEGNPLFVN
                     ATAGDFHLATNSPAMTNGVNLSSLKLPGLSQDKEGNPRPPYGPWPLGAYQTSIVGPLP
                     PTGLRVILN"
     gene            8277..8765
                     /locus_tag="VG754_11570"
     CDS             8277..8765
                     /locus_tag="VG754_11570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWF19902.1"
                     /translation="MRLGKKVIVARDCWIQTIGKSAEDFEVKLIIGAQTGIGMGASIS
                     AARMVTIGQNVLLARNVYISDHAHAYENIRVPIMKQGITEPKPVRIGDYTWLGQNVVV
                     LPGVSIGQHCVIGANSVVNKSIPDFCVAAGAPARVIKRHNPETGTWESVRNTEDSSAV
                     AQ"
     gene            8856..9701
                     /locus_tag="VG754_11575"
     CDS             8856..9701
                     /locus_tag="VG754_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF19903.1"
                     /translation="MQELVLEAGRSERHYWRDLWRYRELFYFLAWRDLLVRYKQTFVG
                     VAWSLIRPLLTMVVMTVVFGKFGKMPAGGVPYALLVFCGILPWQFFATALSESGNSLV
                     NNSNLISKVYFPRLIVPASSVVVSFVDFLVSAAFLLALMMWYHYFPAAGTVSYVPPAN
                     MVALPAFLLLAIAASLGAGLWIAALMVEYRDFRIIVPFVVQFGFFITPVAYMFPRQYE
                     QWHLLYSLNPIVGVIEGFRWAILGGEHRLYWPGLLISMAGTILMLVSGIWHFRKTERT
                     FADVI"
BASE COUNT         2149 a         2429 c         2839 g         2404 t
ORIGIN      
        1 cacggacgct tttcccgtcc tgcaacaaca tgggttcagc gccacgatgt tcctaccgac
       61 ggcgttcatc cgcgagaaga ggcgaatgtt caaggaacgc gaatgcatga cgtggaacga
      121 agcgcgcgag ttgcatcggg ccggaattga gttcggttcg acaccatcaa tcatcccaag
      181 atttatgaac tggatttcag cgacattcgc accgagctcc aggtttccaa atgggtgatt
      241 gaggacaaac tcggcgtggc gatttcatca tttgcatatc cttatgcttt tccaagcgcg
      301 gaccgcgact ttgtggaaac gctgaccgac ttgttgaaag gttcgggtta cgagtgtaat
      361 gcgaccacac gcatcggccg cgtgcatgcc gaagatgatc cgtacatgtt gaaacggttg
      421 ccggtcaatt ccgccgatga cagcgcattg tttctcgcaa aaattcgcgg cgcgtacgat
      481 tggatgaact ggccgcagga ccgattgaag aaattgaaag tcgccgcaaa cggacgcaca
      541 caacagcgtc tctgccggac gccataaatt tcaagagaat gatgcgaacg aaaacctgtc
      601 ctaattacgt tttggtgacg cctgtcaagg atgaggaagc tttgatcgga aagaccgtcg
      661 aatctgtggt gaaccagacg gtgccgcctt cagaatgggt catcgtaagt gacggatcca
      721 cggatcgaac cgatgcgatt gtgcgagagg caggggtgat gcacccatgg attcgcctgg
      781 tgcagttgcc tgtgcgccag caacggagct ttgcggcggt cgttcgcgcc gtggaaaagg
      841 gtgtggcttc attgcagaag aaagattacc aatatatcgg gcttctggat tcagatcttc
      901 gttttaagcg tgattatttt gaaaaggtga tcgagcattt tgaatgcaat gtccaactcg
      961 gtcttgcggg cggcgtcgtg atagacgtgg attcacccga acagcggttg ccgcgaaatc
     1021 gtcaggatgt tcccggggcg gtccagtttt ttcggcggca atgtttcgag ggtcttgacg
     1081 gacttctggg gattcctgag gggggctggg acgcgctgac ctgtgcacga gcgcgcatga
     1141 ttggttttga aacccgactg ctgacggaac tgattgttga ccatctgaaa cctcgcaatg
     1201 tttcggaagg cggcttgctt cgccggcaat ggcagatggg aatccgcgat tatgcgctgg
     1261 gttatcatcc tctttttgaa ctcttcaaat gtcttgctag aatggggcag aaaccttttt
     1321 tcatcggctg catcgcatgg tggatggggt attgctccgc ggcaatccag cgccgggaac
     1381 ggttcattcc caaggaactg cttaacttcg tgcgcggtga gcagaagaga cggcttgttc
     1441 gggctctgat tggttcttga gcttcttcat ggtgtcatca cattatggaa tcacacattg
     1501 taaaaactga accggatgaa tcgcatagcg ggtgcgatgt gcccgttaga cgagacgcag
     1561 ttgcaaattc atcacccgcg cgcaacggag aagccatctc catcaaggac cgattgaagc
     1621 gtctgcctgg aggcgttttg tgggtgatgc ggttttttat aattcgcccg ttaagaattt
     1681 atttcaggta ctgtccggtt catttcggaa aggccgccgt atgggatcgg accgcagcgc
     1741 atctttggtg gttggagtcg gaggtgaggg ggcggacagt ttttggcagc aagcttgggg
     1801 ttgatgccca agacattgtc gggaaataca tttatcattt tggcatttgg gagccaaatt
     1861 tgacggactg gatgacgcat cgactcaaac caggcgatgt cttcattgat gtcggcgcga
     1921 acgtcggtta tttttcatgt ctcgcctcga agctcgtggg tgattccgga aaggtggtga
     1981 gcatcgaagc cctgcctcag atttttgaga ggctcaagga gaacctggcg atgaacgggg
     2041 cgcacaacgt gcgacttgca aacgtcgctg tgtggaacaa ggaggagaac cttaagatgt
     2101 tcacccggat ggcgcaccca ttgggagcaa ccagtttaat gccggcttgg gcggcccatt
     2161 ggcaattgga aacagcggtt gaggtgccgg caaaaccatt gccggcgatt ctcacgcagg
     2221 aggaaatcac gcgagcgcgc ttgataaaga tcgatgtgga aggcgccgaa tggaaggtca
     2281 tttcagggat ggcggacatg ctgccggatt ctcgccagga tttggaaatc atcgttgaga
     2341 tttcgcgcgg catgttgaag gagcagggca aaagcgcggg cgacttgctg gaattatttg
     2401 ccaggcatgg ttttcatccc taccgcttga tcaatgacta ctccgtcggg gcctatgtgg
     2461 cgcccaggat tccacagccg ccgaaacggg tcgagacaat ccttgacagc gccgaagatc
     2521 aaatggacgt gattttttcg cgggccggtg gagcgtcgct ttagacgatc gcccgcaatc
     2581 aatgactgtc cacaacctct tttaaattca gtggatcgcg atgaacgaga aaagtaaacg
     2641 tatccgggtc ttgtacagct ttccgctgaa aatcggcgca ggcagggttt gcaccatcgc
     2701 ctggtatcag gctcaaggac tggccaatgc aggagcggaa gtgacggtct ttcctgcctc
     2761 catttgccgg gattttacct ctgcggtgaa ggtccagcca accctcgcgt ggggaaagtt
     2821 ccggttgccg aaccgattgg tcgggcggag ggcgtatgcc atcatgcatg accgcattgt
     2881 ggcgcggcgg cttgaaaaaa tcgctgaccg catagacatc atccatacat ggcccctggg
     2941 cgcgcgggag acgcttaaag ccgccgccaa actcgggatt ccaacggtct ttgaacgatg
     3001 caatgctcat acaaggttcg cttacgacgt ggtcaacaag gaatgcgagc gcatcggagt
     3061 cccgctgccg ccgggccatg aacatgcctg gaacacagag ttgctcgaaa gagaggaggc
     3121 cgaataccaa ttagcttacc ggttgctttg cccgtcggaa tttgtggtga agactttcgt
     3181 ggaccatgga tttgcccgtg agaagcttgt ccggcacatc tacggcttcg accaaaagat
     3241 tttttacgcg gacaacaagc cacgcgaccc gaagcgcggg ttgacgatga tatccgtcgg
     3301 agtgtgcgcc gtgagaaagg gtttgcactt cgcattggaa gcatggctca aatcaccggc
     3361 ctcgcgcgac ggcacttttt tgatagtggg cgagtttctg ccggcttacc gggaaaagct
     3421 cgcttccatg ctggcgcatc ccagtgttaa agtgcttggc catcgcaacg acgtgcccga
     3481 gttggtgcgc caaagcgacg ttctggtgtt gccgagcgtc gaggaaggtt tcgggctggt
     3541 cgtcacggaa gcgatggcca gcggttgcgt gccgctgatt tccgatgcat gcacagacct
     3601 ttgcaagcac atggaaaacg gaatggtcca ccgtgtgggg gatatgggga tgttggaacg
     3661 gcacatcgcc actttgcatg aagaccgcgg gctgctggaa aaattgcgcg ccgcgggatt
     3721 gcgccttgcg cccgagatca cttggaacgc cgctggcgtg aaactgctgg atgcttatcg
     3781 tgaagtgctg gcggcggccg gcacgacccg gtcacaccgc tgtgtggggg acaaagatga
     3841 aagtgaacgg gtgaaggcat gaatgcagcc ggagcaacga tttgctcaat cttggttctg
     3901 gtggtgctga tcgctcccag gcgatgggca ttgctcggca tgattgccgg ggcattattt
     3961 ctcacggaac gtcagcaagt ctcggtggga gggataaatt tctactccat acgatttttg
     4021 gaattggccg gatttgttcg ggtcatgtcg cgcgctgagt tttcttttcg ctcatgcaat
     4081 cggatagatc gtgcattgat atttttatac gtttacacag cggtggtatt ctgcctgcgt
     4141 tccgcggagg ggcaggcata catcattggc aactcggtgg acgcctttct ttgttatttc
     4201 acgttccgcg ggctggtgcg ggatgccagc gagcttaaac tttttctgcg ggattttatc
     4261 atgctccttc tgccgtacgc agcgatgatc ttgtacgaat cgatgaccca ccggaacgcg
     4321 ttcgcgttta ttgacggagg gatgtacaac tgggtgaggg gaaaccggtt ccgctccgtg
     4381 ggcaccttcc gaaatccgga caccttgggc acgctgggag cagtttttgc ccccttatac
     4441 tttgccattg cgttcgcgca acgtgaacga aagttgccca tcttcggcat gttgctttgc
     4501 ttgtgtatcg tttggggctc gaattccggc gggcctttat gcgcgacggc tgtcagtttt
     4561 gtcgcttggg gattctggcg catgcgcacc aggatgcaga cggtgcgccg gacaatggca
     4621 gcgattttga tcctcctcgc ctgcgtgatg aaagcgccga tttggttttt gctggctcgc
     4681 gccagcagcg tcgcgggtgg agatggatgg catcgggccc agttgatcag cgtggccttt
     4741 aaacacctga acttgtggtg gtttgccggc atgcctctga gcgacacgtc cgagtggttc
     4801 gaatacagca ttggaggcgc agcggacatt accaacacgt ttctcagctt tggtctcaat
     4861 gccgggatgg gagccattat tgtgctggtt cttctgctgg tgcggacgtt tagcggtctg
     4921 ggaaaggctc tgaacagcgt acgctcgaat ggtcaggaag acgactaccg ggaggccatg
     4981 ctttggggat tgggggccgc gctcatggcc catatcgcca actggcaggg gatagtttat
     5041 tttgatcaaa gctacatggt ctggttcctg caactcgccg ctatgacgag tgtttcgtcc
     5101 gcttgcatcg aggctgacga agccgaaaca gttcgagttt cgccagtcga ggaggtgcat
     5161 gcctcacgcg gcgaagaagt ccgaatataa gcgcaataac tatgctggtc agtgtcatca
     5221 ttcccactta caaccgcgcc agaaccattg agcgagcgat caacagtgtg ctctcacaaa
     5281 cttggacccc cattgaaatc attgtggtgg acagctggtc caccgatcgg accgttgaat
     5341 tgcttgcgca gtatggagac aaggtgcgtc tcatcagcca ggaaaaaatg gggcctggct
     5401 ttgcgcgaaa tgccggcatc aaggcggcta aaggagaaat tatttcattt ttggattcag
     5461 atgacgaatg gttgccggaa aagaccgcgc ggcaggtaaa actgctccaa gcctcaggcg
     5521 tcaattgttg tgtctgcaat gcccgcatgg agttcatctc gggagcggtc aattcgttcg
     5581 atgtctcgcg gctgcgctcg aagctgcggg aaggcatttg gaccaaccca accgaggtgc
     5641 tggtcacccg ttttcttttc ttcaatcaag tggtggcact tcgacgcgag gcgcttgagc
     5701 gaaccggtta tttccggcaa gggatcatgg aagattatga tctcgcgttg cgcctctcct
     5761 tgctggggcc gtgggctttc attgccgacc ccttggtggt ttggcatgag caggccggcg
     5821 acaatctctc aaaaacccac agtcaactgg aaatctgcca gcggaccctg gaaattttgg
     5881 aggaaaccag cagttcgtct caatggggtt cgctgttgcc ggaaaaattg acccgttcgc
     5941 gcatgaagtt tttaagcaaa cgcgttgaag cattgcgatt atcggcgctt tctaatccgc
     6001 tggccaggtt atcgggcaag tttcgcctcc aacttctgca cactggtcta aagctctatt
     6061 ggcggctgcc ttcgactcca cgtatgaaca cccgtgcggt gtaagtattt tgggcggcta
     6121 agccgactca tgtctccaaa tgagacggaa aacgccgccc caaaagaaaa tgcaacgatt
     6181 tgcggttcgg cgcgactttt gctaccattg cccgtggcac ggtttggctc agtcagggcc
     6241 aaacagggac cgccgacaaa gaagacattt cagataatgc tatgcaggcc agcccagaat
     6301 ttgatataaa atacgattaa agcctgtcat ttcttttgaa gtagtatgtt aacttatttg
     6361 attcgtatcg tgaagaaagc cttcgtaacg gccgcctctt ttaccttttg ctggcatctt
     6421 ggagcagctt cctatttcgt ggattatgcg gggggggctg attcaaattc cggcacagca
     6481 accaacgccg catggaagca ttgcccgggt gatgttgtgg ctacggccaa tcccgcaagc
     6541 accgtcctga tgccgggaga tacggttttt ttcaagggcg gtgtgacgta tgtcatcatc
     6601 ccccagacag cgggagggcc tggaatctat ttgaattggg ccggtgcggc aggaaatccc
     6661 attaattacg acgggaacag cggtgggact tggggatccg gcaaggcgtt gatgaccgat
     6721 aactacaagg gttctgtcgc tttttattcg cccgcaggaa tgacaagcct ggtcttcaaa
     6781 aatttcgcca taggtccgat gggaggtgcg gcagtgcttc cttctgatcc gaaagggaca
     6841 aattatgctc ccaatggcat ccctcccaat ccgggcggcg gtttcgtttg cggtggaatg
     6901 atcaacagcg ttgtcatttc cagttgcact ttttcaaatt gcggttatta tggctcgaca
     6961 tatccccttg gaaacgcttc gctcgaaggc ggggcgcttt catgcaacgg gtgcaacggt
     7021 tttaccatca cgaattgcac cgtgtctcga atgcgggatg gaatttacct cagcaacccc
     7081 agccaaatat ccaatctcac gattgctgga tgcacattta accagattga ggaatggggc
     7141 atcacgatga atacgggtgt gaacgcttat ttgagcaacg tttacatttt caactgcctt
     7201 ttcaccaatg tggaccaaaa ttatgcggct tggggcgggt acggaagccc tccgcatcgt
     7261 gacacaatct tcagttttgg agccgaaggt tgcaacggca gttgctcgct ccagcgaaat
     7321 gtgactgaca ccaatgtgtg catttataac aattatttcg tggataccat gggatatgtt
     7381 ggaggaacgg cggtggtctg gtttacagac aatgccagcg cgaatattta caacaatgtg
     7441 ttcgtgaaca cgggagaggc caatgcggct gtcaaactga atggcccatc cgacaattcc
     7501 caatggcgcg cggctgttta caacaatacg ttttatatgc aggactatga aattcctatc
     7561 agtttcggtt cctgtcctgt caataactct tcgtggccga tcagtccaac caaccacaac
     7621 ccgatgatca ccatcctgaa caacaacgtc tattcatggt cgggcgcaac tccctatgtc
     7681 aatggctgta atgtgctgat cgaagattcg acccccaatt tgacgaacaa cgtcgttctt
     7741 gattataatt tgtatcgctc aagcctcgca gtgaacagtc agatggtcta tgcgcagtgg
     7801 ccattgtatg gtggttatat cgtccagaac gtggcccaaa tcctatatgg ctgggacgtc
     7861 cacagcattg agggaaatcc cctgttcgtg aacgccacag cgggcgattt tcatctcgca
     7921 accaacagcc ctgcgatgac aaatggggta aacctgagca gcctcaaatt gccgggatta
     7981 tcccaagaca aagagggtaa tcctcgacct ccttacggtc catggccgct gggagcgtat
     8041 caaacctcca tcgttggtcc gctcccgccg acgggattgc gggtcatctt aaattaagcg
     8101 tcttcttcgc gccgggcaga ttgatcgtaa tgaccattgg ggcaatagag cggacgctgc
     8161 atttagcggg gcgtctgcac accaggtttt ttacgacctt gatttccgga caatttcggg
     8221 agttcggcca cggttcccgg gtcagccctc cttttcgatt ttatggcctg caccggatgc
     8281 gattggggaa aaaagtgatc gtcgcgcgcg attgctggat tcaaaccatc ggcaaatccg
     8341 cagaggactt cgaggtgaag ctcatcatcg gcgcgcaaac gggcatcggc atgggagcca
     8401 gcatctccgc ggcacggatg gtgacgatcg gccagaatgt gctgctagcg cgcaatgttt
     8461 acatctcaga ccacgcgcac gcctacgaaa acatccgggt gcccatcatg aaacagggaa
     8521 tcacagagcc aaaaccggtg aggattggag actacacatg gctgggacaa aatgtggtgg
     8581 tgctgccggg tgtttcaatc gggcaacatt gcgtcatcgg agcgaactcg gtggtgaaca
     8641 aatccatccc ggacttttgc gtggccgcgg gggccccggc gagggtcatc aaacggcaca
     8701 atcccgaaac cggcacgtgg gaatccgttc gcaatacgga agattcgtcc gcagtggctc
     8761 agtaatgaga cggcgctcgc gatgaatttc gcaagtccga catcgctgtc caaaggtaat
     8821 tatccaacag gttagaatcc caaacgaccg attccatgca agaacttgtc cttgaagcgg
     8881 gccgctccga acgccattac tggcgcgatt tgtggcgcta tcgtgaatta ttttatttcc
     8941 ttgcgtggcg cgatcttttg gtccgttaca aacagacgtt tgtgggggtc gcatggtccc
     9001 tgatccggcc gttgctgacg atggtggtga tgactgtggt gttcggcaaa ttcggcaaaa
     9061 tgcccgccgg cggcgtgccc tacgcgctgc tggtgttttg tgggatactg ccgtggcaat
     9121 ttttcgcgac ggcgctttcg gaaagcggca acagcctggt gaataattcc aacctgattt
     9181 ccaaagtcta ttttccgaga ttgatcgttc ctgccagcag tgtggtcgtg agttttgtgg
     9241 attttctggt ttcggcggcc ttcctgctcg cgctgatgat gtggtatcat tattttcccg
     9301 ctgccggcac tgtgtcgtac gttccgccgg caaacatggt ggctttgcca gcattcttgc
     9361 tcttggcgat tgccgcgtcc ttgggggcgg gcctttggat tgccgcattg atggtggaat
     9421 atcgcgattt tcgcatcatc gtgccgttcg ttgttcagtt cggttttttt atcacgccgg
     9481 tggcttacat gtttcccaga caatatgagc aatggcattt gctctactca ttaaatccca
     9541 tcgttggtgt gattgaaggc tttcgctggg cgattctcgg cggggaacat cggctttatt
     9601 ggccgggatt gctgatctcc atggcgggga caatattgat gctcgtgtcc ggcatctggc
     9661 atttcaggaa gacagaacgg actttcgcag atgtgattta gcgtggcggc agttgttttt
     9721 taattccaat caatgagtga tacggtcatc aaggtcgaaa acgtctcgaa acgctatgtg
     9781 gtcggcaccc ggcgcaattc gcaagacggt ctgcggcacc a
//
LOCUS       DASZMM010000479         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5146201, whole genome shotgun sequence.
ACCESSION   DASZMM010000479 DASZMM010000000
VERSION     DASZMM010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5146201"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VG754_11580"
     CDS             complement(<1..250)
                     /locus_tag="VG754_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HWF19904.1"
                     /translation="MTRFDFKKLIALTVPALLLAGQFQVMGDEPMQKVPRFSVDYMDR
                     SVDPTVDFYHFANGNWLKQNPVPEDKSRWAGFDELQERN"
     gene            745..1152
                     /locus_tag="VG754_11585"
     CDS             745..1152
                     /locus_tag="VG754_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19905.1"
                     /translation="MADINPSLPGGAVPPKPGEAPKVQPKKETVRINLPPKPSAAPTI
                     KLPTLPPGGPSAGAPVTMAPPTAPAAKPASAAPTHAGAAPVAVGAKPMQQRPPPPPAR
                     PAIGALDTGLAIGAAIAGLVAIGTTIYCLMLFT"
     gene            1209..1538
                     /gene="trxA"
                     /locus_tag="VG754_11590"
     CDS             1209..1538
                     /gene="trxA"
                     /locus_tag="VG754_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HWF19906.1"
                     /translation="MASSNIIILTKDNFENEVLKSQTPVLVDFWAEWCGPCKMIAPIL
                     DELADEYTGKVIIGKVNIDDHQNLATEYGVRAIPTLLLFQNGQVAEQIVGLRSKRDLK
                     ASFDKVA"
     gene            1573..2571
                     /locus_tag="VG754_11595"
     CDS             1573..2571
                     /locus_tag="VG754_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF19907.1"
                     /translation="MVTPRQLIHRAQFYRQLGQLTAAGIPIIKALDMLLRNPPARSYS
                     QPIETMVGQLSQGSTIGESLRHLGRWMPSFDIALVEAAEKSGRLDSVFRMLADYYEDR
                     AALMRRVIGDLIYPIFLFHFAVLIFGFVEWFQGGASLMSFALSILKILIPLYVVLFLL
                     VFAMQSRRNEKWRSFLERLLRPVPVLGPARHCMALSRLSAALEALINAGVSIVEGWEM
                     AAAASGSPAIYRAVIAWRPKVNAGQTPSEAVNAARSQFPELFANLYYSGEVSGQLDDS
                     LRRLHLYYQEEGTQKLRLLSLMVPKAIYLCVAGMIAYKIIHFYTGYFNEIKKVIGP"
     gene            2581..3735
                     /locus_tag="VG754_11600"
     CDS             2581..3735
                     /locus_tag="VG754_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWF19908.1"
                     /translation="MTLNEILNNEELRNHEFPVTRERIFLAHAGDCPLPRRVADAISA
                     YVRQASAADQEMCVYPAILETGRKLAARLLNSQPEEVAFVGPTSLGLSYVAAGLNLRR
                     NDNILIYFDDYPSNVYPWMALAERGIQVRLINTRELGVIRPIDVMGQVDEQTRLVALA
                     SCHFITGTRLDHQAIGKFLRERNILFCVDGIQTLGAFPTTVENVDFLAADAHKWLLGP
                     CAAGLFYVRHALQEKLRPPIYGWNNVRSPNFVAQEQIVFRKDAHRFEAGTHNLLGIVG
                     LVAAMELILEIGVENIAAELLRKRAWLVPALQAKSYKVVRANDTPENASGIVSFFRTD
                     GGDLAELHQKLMDANVITSLRTDRKGQHYIRLSPHFYNTDTELQRVLELL"
BASE COUNT          795 a         1066 c         1055 g          851 t
ORIGIN      
        1 agttgcgttc ttgcaattcg tcgaagccgg cccaacgcga cttgtcttca ggcaccggat
       61 tttgcttgag ccaattgcca ttggcaaagt ggtaaaaatc aacggtgggg tccaccgagc
      121 ggtccatgta atccacggaa aagcgcggca ccttttgcat gggttcgtcg cccattactt
      181 gaaattggcc ggcgagcagc aatgccggga ctgtgagagc aatcagtttt ttaaaatcaa
      241 atctggtcat gcagtttacc tttcgcggac ggaagcaaag gggaaacata agcccaaact
      301 gctgggatgc aagataacgc ttttgaacac cgcgcgtctg gacgcatctc agtttcttgc
      361 acaacggagt gattgaacat ttccttcccc ctcaccccgc cctctccctt ggggagaggg
      421 ctgggtgagg gggacagcga tgtggccgac ccataaacac aattcgcaag agccccgaaa
      481 cgtgcaaaat agtgagatgc gccccgcgcg tccggacgca tccccgagtt gcttggagcg
      541 cggggtcggt gacccgcagc aatgtgggaa tgcggatgcg tatgcattaa tctggcgcgt
      601 cctgaacttc gcgccttgct gcggctcagc gagccgcacc ccggtttcct gatgaacacc
      661 ctgaccgtcc gtaactttac cgactattaa gagtttgaca ccggcccaag gcaactttaa
      721 tttggcgtta caaaacgtaa aattatggct gatatcaatc caagtctgcc cggcggcgcg
      781 gttccaccca agccgggaga agctcccaaa gtccagccca agaaggaaac ggtgcgcatc
      841 aatctgccgc ccaagccctc ggcagcgccg acgatcaaat tacccacttt gccacccggc
      901 ggtccgtccg ctggcgcgcc tgtgaccatg gcgcctccca ctgcacctgc ggcgaagcct
      961 gcttcggccg ctcccacgca cgccggcgct gctcccgttg ccgtcggggc caaaccgatg
     1021 cagcaacgtc cgcctccgcc gccggcacgc ccggccatcg gcgcgttgga cactggtttg
     1081 gcaattggcg cggcaattgc cggattggtc gccatcggca cgacgattta ttgtttgatg
     1141 ttattcacct gagccacgct ttcacaacaa tacctatcct gtcgcaaacc catacccagc
     1201 atttaacaat ggcttcctca aacatcatca tcctgaccaa ggacaatttc gaaaacgaag
     1261 tcctcaagtc ccaaactccg gttttggttg atttttgggc cgaatggtgc ggtccttgca
     1321 aaatgatcgc gccaatcctc gacgaattgg cggacgaata cactggaaaa gtcatcatcg
     1381 gcaaggtgaa cattgacgac caccaaaacc tggccaccga atacggcgtg cgcgcaatcc
     1441 ccacgctcct cctgttccag aacggacaag tcgccgagca gatcgttggc ctgcgcagca
     1501 agcgcgacct gaaggccagc ttcgacaagg tcgcttgatg agcggcctcc gggcgcggac
     1561 gccataccgc acatggtaac gccccgccag ctcatccacc gcgcgcaatt ttaccggcaa
     1621 cttggccagt tgaccgccgc cggtattccc atcatcaagg cgctggacat gcttttgcgc
     1681 aatccgcccg cgcgctccta ttcgcaaccc atagaaacca tggtcgggca attgtcgcaa
     1741 ggctcgacca tcggggaatc attgcgccat ttgggccgat ggatgccttc gttcgacatc
     1801 gcgctcgtgg aggccgcgga aaagagcgga cggttggatt ccgtgttccg gatgcttgcg
     1861 gattattatg aagaccgcgc cgcgctgatg cgccgggtga tcggtgattt gatttacccg
     1921 attttcttgt ttcatttcgc cgtgctgatt tttggtttcg ttgaatggtt ccaaggcggg
     1981 gcgagcttga tgagctttgc attgagcatc ctgaaaattt tgatcccgct ctatgtcgtg
     2041 cttttcctgc tcgtttttgc gatgcaaagc aggcgcaacg agaaatggcg gtcattcctt
     2101 gaacgattgt tgcggcccgt tccggttttg ggaccggcgc ggcattgcat ggcgttgtcg
     2161 agattgtccg cggcattgga agcgctcatc aacgcgggtg tgagcatcgt ggaaggttgg
     2221 gagatggccg ccgccgcgag cggttcgccg gcgatttatc gcgccgtcat cgcgtggagg
     2281 ccaaaagtca acgcgggaca gacgccttcc gaggctgtca acgccgcgcg ttcgcaattt
     2341 cctgaattgt tcgccaatct ttattattcg ggcgaggtca gcggccagtt ggacgattcg
     2401 ctccgccgat tgcatcttta ttatcaggag gaggggacgc aaaaactgcg cctgctctcg
     2461 ctgatggtgc ccaaggcgat ctatttatgt gtggcgggaa tgatcgcata taagatcatc
     2521 cacttttaca cgggctattt caatgagatc aaaaaagtca ttggcccttg atcggtttcc
     2581 atgacgctca atgaaatcct gaacaacgag gagctgcgca atcacgaatt tccagtgacg
     2641 cgcgaaagaa tttttctcgc gcacgcaggc gattgtccgt tgccgcggcg cgttgcggac
     2701 gccatttccg cttacgtgcg acaagcgagc gctgccgacc aggagatgtg cgtctatccc
     2761 gcaattttgg aaacgggacg caagctggcc gcgcgcttgc tcaattcgca accggaggag
     2821 gtggcgtttg tcgggccgac ttcgcttggg ttgagttacg ttgccgccgg gctcaacttg
     2881 cgcaggaacg acaacatcct gatttatttc gatgattatc cgtccaatgt ttatccatgg
     2941 atggcgcttg ccgagcgcgg catccaggtg cggctcatca acacgcgcga actcggcgtc
     3001 atccgcccta ttgacgtgat ggggcaggtg gatgagcaaa cgcggttggt tgcgcttgcg
     3061 tcgtgccatt ttatcaccgg cacacggctg gatcatcagg ccatcggcaa atttttgcgc
     3121 gagcggaaca ttcttttttg cgtggatggc atccagacgt tgggcgcatt tccaacgacg
     3181 gtggagaacg tcgattttct cgcagccgat gcgcacaaat ggctgcttgg cccgtgcgcg
     3241 gcgggtttgt tttatgtgcg gcacgcgctc caggaaaaat tgcgcccgcc gatttacggc
     3301 tggaacaacg tgcgctcgcc gaattttgtc gcgcaggaac agatcgtgtt tcgcaaagat
     3361 gcgcaccgtt tcgaagccgg cacgcacaat ttgctcggca tcgtgggatt ggttgcggcg
     3421 atggaattga ttttggaaat cggcgtggag aatatcgcgg ccgaattgtt gcgcaagcgg
     3481 gcatggctcg tgcccgcatt gcaggcgaag agttacaaag tcgtccgcgc gaatgacaca
     3541 ccggaaaacg ccagcggcat tgtttctttt tttcggacgg atggcggcga tttggccgaa
     3601 ctccatcaaa aactcatgga tgcgaacgtc atcacttcgc tccgcacaga ccgcaaaggc
     3661 cagcattaca tccgcctctc gccccatttc tataacaccg ataccgagtt gcagcgcgtg
     3721 ttggaattgc tctgaaattt tcggaatgtt tcaaaaagca ttactgt
//
LOCUS       DASZMM010000480         5328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5436343, whole genome shotgun sequence.
ACCESSION   DASZMM010000480 DASZMM010000000
VERSION     DASZMM010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5436343"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..571
                     /locus_tag="VG754_11605"
     CDS             314..571
                     /locus_tag="VG754_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19909.1"
                     /translation="MALICAATVTSWNVQAQKAVTDEQKALKKEMLEKYDTNKDGKLD
                     KDERAKMTPEDKAKWSKAFPHKKKKTDDGSSSSTTPAPETK"
     gene            complement(741..3116)
                     /locus_tag="VG754_11610"
     CDS             complement(741..3116)
                     /locus_tag="VG754_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HWF19910.1"
                     /translation="MSVAFLLAFSSFGLAAEPSASDVQFFENKVRPILVDNCYKCHSH
                     EAPKLKGGLSIEFRESLLKGGDTGPAIVPGDPEKSLLIKAIRYGDPDLQMPPKDKKLS
                     DDQIQTLTTWVKMGAPYPANAALAKNAGASERDHWAFKPIRKYSIPEVKETNWVSTPI
                     DNFIVATLEENGMKPSPMADKRTLIRRATFDLIGLAPTLKETEDFLKDDSTNAFAKVV
                     DRLLASPQYGERWGRYWLDVARYSDTKGEVKQQQEDFRYPFAWTYRDYVIRAFNEDKP
                     YNRFVLEQLAADKLPLSADRHELAAMGFLTVGDHFNGMQNDILNDRIDVVTKGFLGLT
                     VTCARCHDHKFDPIPTKDYYSLRGIFVSCVEPKDEPILGKLEMTPQYNEFATKYNELN
                     QQLTALMAMKRGGKNKEIRKKETELRRDIAMLELNHPGSPPRAMVLVDRPKPSDSPVF
                     IRGEVDNQGPVAPRRFLEILSGPNRPAYRSGSGRLELAYDIIRPSNPLTARVMVNRIW
                     LHHFGEGIVTTPDDFGNQSAPPSHPELLDYLALRFMDDGWSIKKMHRLIMLSSVYQQS
                     SDNNPRYAQIDPNNRLLWRANIRRLEFEAVRDSLLAMGGRLDLTMGGHPVNLGATPYS
                     TRRTVYGYIDRRNLPEVFNQFDFANPDIETGKRYETIVPQQALFMMNSPLVVEQARNL
                     VNRPDFKSTTGAKERIKLLYDVIYQREPTDVEIQLGLNFLTETPPPETPTEPVRFDRA
                     QRQKDKKGNGKKGLGTAMSLATIPPAGLMPVGAWAKYAHALLQANEAMFIN"
     gene            complement(3461..4879)
                     /locus_tag="VG754_11615"
     CDS             complement(3461..4879)
                     /locus_tag="VG754_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF19911.1"
                     /translation="MNINEHVARPEDFAITRRQFLNRFGMGLGALGLATLLGPEMFGG
                     VARADDTQFNPLAPKNPPFPAKAKHVIHVFAQGAPSQVDTWDPKPALAQYADKSLPGM
                     SGTAFASPFKFNKMGRSGIDVSEVFPKIGEHVDDMAIIRSMYTDIPAHEVATVMMNTG
                     SLRLAKPCLGSWAVYGLGTDNQNMPGFISLRPGGGLPAGGTQNWQAAFLPGVYQGTSV
                     NTQATSVEDMIQNIKNPYISLTEQRRQLDLIHQLNELHSQNLQKDAQLEARIEAYEIA
                     FKMQSAASDAFDFHKEPQEMQEMYGRTTQGRQLLIARRLVERGVRFVQVWAGGWDHHQ
                     DIEDRLPKSAAEIDQPLGALLTDLKQRGLLDSTLVIWGGEFGRTVTRDRNGNDNPGRD
                     HNNKAFSTWMAGGGVKGGTIYGATDEFGAKAVENKVHIHDLHATILALLGFDHTKLTY
                     RYNGRDFRLTDVYGNVVKGVMA"
BASE COUNT         1193 a         1497 c         1468 g         1170 t
ORIGIN      
        1 cacttgccgc actcttcagg aaaaatcgca ctccggccag atgtccaccg gctccggctc
       61 cggcgcgttt ccgtatgtca cgatgggcat cgtttttggc ctgggaatgt tgatgggcgc
      121 cttgctcaag cgtgattccg acgagggcga agcttaggct cataatctcc cttacaccgg
      181 gcaaaagacc tcccggacca gtcctcccaa aatcctgtaa cctgccagcc acgggtgtcg
      241 tctggttcga tacgacagtt taaacaacaa caatcaacat attacaaaga cagatatgaa
      301 gaaattcgtg gcaatggcac tcatttgtgc ggcgaccgtg acgtcttgga acgtgcaggc
      361 ccaaaaggcc gtgaccgacg agcagaaggc cctcaaaaag gagatgctcg aaaagtatga
      421 taccaacaag gacggcaagc tcgacaaaga cgagcgcgcc aagatgacgc cggaagacaa
      481 ggcaaagtgg agcaaggcct tcccgcacaa gaagaagaaa accgacgacg gcagcagcag
      541 ctccaccact cctgcgcctg aaacgaagta atttctgacg cgtccaatct cagaaggcca
      601 cctcgggccc aaacgaacaa aacgctccgc ctaaaaaacg gggcgtttta ttttgtcctg
      661 agattccgac gaccggagca ggaacggaat agtggagtat tattcttccg ttcaacaccg
      721 caatactcca gcactccact tcagttgatg aacatcgctt cgttggcttg taggagcgcg
      781 tgcgcgtatt tggcccaagc gccgaccggc atcaatccgg caggcggaat cgtcgcgagt
      841 gacatcgccg ttcccaagcc ttttttgccg ttgccttttt tgtctttctg ccgctgcgcg
      901 cgatcgaaac gcaccggttc cgtcggagtt tcgggcggag gcgtctcggt gagaaaattc
      961 aatccaagct ggatctcaac atccgttggt tcgcgctgat aaatgacgtc gtagagaagc
     1021 ttgatgcgct ctttcgcgcc agtggtgctc ttgaaatcag gacggttcac aagattgcgc
     1081 gcctgctcga caaccagcgg actgttcatc atgaacaacg cctgttgcgg cacgatggtt
     1141 tcataacgct tgcccgtttc gatgtccgga ttggcgaaat caaactgatt aaaaacctcc
     1201 ggcaggtttc tccggtcaat gtaaccgtaa accgtccgcc gcgtggaata aggcgtcgcg
     1261 ccaagattca ccggatgccc gcccatcgtg agatcaagcc tgccacccat cgccagcaat
     1321 gaatcgcgca ctgcctcgaa ttcaagacgc cggatgttgg cgcgccaaag caaacgattg
     1381 ttcggatcaa tctgcgcgta acgcgggtta ttgtcgctgc tctgctgata aacgctggaa
     1441 agcatgatga gccggtgcat tttcttgatg gaccagccgt cgtccatgaa gcgcagagca
     1501 aggtaatcaa gcaattccgg atggctcggc ggcgcggatt gattgccgaa atcgtccggc
     1561 gtggtcacga tgccttcgcc gaaatgatgc agccagatgc ggttcaccat cacgcgcgca
     1621 gtgagcggat tgctcgggcg gatgatgtca tacgccagtt ccaaacggcc gctgccgctt
     1681 cgatacgccg gacggttcgg gcctgaaaga atttccaaaa aacggcgcgg cgcaactggc
     1741 ccctggttgt ccacttcacc gcggatgaac accggcgaat ccgagggctt gggccggtcc
     1801 accaaaacca tcgcgcgcgg cggcgaaccg ggatggttca attcgagcat cgcgatgtcc
     1861 cgccgcaact ccgtttcctt cttccggatt tccttgttct tgcccccgcg tttcatcgcc
     1921 atgagcgcgg tcaattgctg gtttagttcg ttgtatttgg tcgcaaattc attgtattgc
     1981 ggcgtcatct ccagcttgcc caggatcggt tcgtccttcg gctcgacgca gcttacaaaa
     2041 atgccgcgca acgaataata atccttggtc ggaatcggat cgaacttgtg gtcgtggcag
     2101 cgcgcgcaag tcaccgtgag ccccaaaaac ccctttgtca ccacgtcaat gcggtcgttg
     2161 agtatgtcgt tttgcatccc gttgaaatga tcgccaaccg tgagaaatcc catcgcggcg
     2221 agttcatgac ggtcggcgct gaggggcaat ttatctgccg cgagttgttc gagcacaaag
     2281 cggttgtaag gcttgtcttc attgaaggcg cggatgacgt aatcacgata agtccacgcg
     2341 aacggataac ggaaatcttc ctgttgctgc ttcacctcgc ccttggtgtc cgaataacgc
     2401 gcgacatcga gccagtagcg gccccaacgc tcgccgtatt gcggcgaagc cagcaaacgg
     2461 tccaccactt tcgcgaaggc attggtcgaa tcgtccttta gaaaatcttc cgtctccttg
     2521 agcgtcgggg cgagaccgat gaggtcgaac gtggcgcggc gaatcaacgt gcgcttatcg
     2581 gccatcggcg aaggcttcat cccgttctcc tccaacgtcg cgacaatgaa attatcaatc
     2641 ggcgtggaaa cccaattcgt ttccttcact tcgggaatgg aatatttcct gatgggcttg
     2701 aacgcccaat gatcgcgctc ggatgcgccg gcattctttg ccaatgcggc gttggcaggg
     2761 tagggcgcgc ccatcttgac ccaggtagtc agtgtttgga tctggtcgtc ggaaagtttt
     2821 ttgtccttgg gcggcatctg caaatccgga tcgccatagc gaatggcctt gatgagcaaa
     2881 cttttttccg gatcgcccgg aacgatggcc gggccggtgt cgcccccctt gagcaatgat
     2941 tcacggaatt cgatagacaa accgcccttg agcttcggcg cttcgtggct gtggcacttg
     3001 taacaattgt ccaccaaaat cggacgcacc ttgttctcaa aaaattgcac gtccgaagcg
     3061 gaaggctcgg cggccagacc gaaagaagaa aacgccaaca aaaacgcgac gctcaataga
     3121 aaacggatgc atcttacttt tttgcatgcg gcgggccctt ttcggtttgt gattgcgggc
     3181 tggcacgcct cgcggtaccc ctcaccccaa ccctctcccc gagcagaggg agaaccattt
     3241 gtgcgctgct tgaccagttg agcgcatttc gattgcgacc acgatgcaaa atgatgctcc
     3301 ctctcccgat gggtgagggg gaaggaagcg tccaatggcc caccgtgcaa aaaagtggaa
     3361 cccacgctga acgctgaacg ctgaacattg aacgccgaac gccgaattgc agaaactcgg
     3421 cgttggttgt tcgatgttgg atgttcgatg tttcccattt tcaagccatc actcccttga
     3481 ccacgttgcc atatacatct gtgaggcgga aatcacgccc attataacga tacgtgagct
     3541 tggtgtgatc gaagccgagc aacgccaaaa tcgtcgcgtg caaatcgtgg atgtgcacct
     3601 tgttctcgac cgccttcgcg ccgaattcat ccgtcgcgcc gtaaatcgtc ccgcccttga
     3661 ccccgccgcc agccatccaa gtgctgaacg ctttgttatt atgatcgcgg ccaggattgt
     3721 cattgccgtt gcggtcgcgc gtgacggttc gaccgaattc accgccccaa atcaccagcg
     3781 tgctatccag caaaccgcgt tgcttcaaat cggtcagcaa cgcgcccaac ggctggtcaa
     3841 tctccgccgc cgatttcggc aaacggtctt caatatcctg atgatggtcc caaccgcccg
     3901 cccagacttg cacgaagcgg acaccgcgtt ccaccaaccg tcgcgcgatc aaaagctggc
     3961 gcccttgcgt ggtgcggcca tacatctcct gcatctcctg cggttccttg tggaaatcaa
     4021 atgcgtccga cgcggccgat tgcatcttga aagcgatttc gtaagcctcg atgcgcgctt
     4081 ccaactgcgc gtccttctgc aaattttggc tgtgcaattc gttcagttga tggatgagat
     4141 caagctggcg gcgctgttcg gtgagcgaga tgtaggggtt cttgatgttc tggatcatgt
     4201 cctcgaccga ggtggcctgc gtgttgacac tggtcccctg gtaaacgccg ggtaaaaatg
     4261 ccgcctgcca gttttgcgtg ccgccagcag gcaaaccgcc gccgggccgc aacgaaataa
     4321 agccgggcat gttctggttg tccgtgccca aaccataaac tgcccacgat ccaaggcatg
     4381 gcttggccag gcgcaacgaa ccggtgttca tcatcacggt ggcgacttcg tgcgccggaa
     4441 tgtccgtgta catcgaacgg atgatggcca tgtcatccac atgctcgcca atcttcggaa
     4501 acacttcgct cacgtcgatg ccggatctgc ccattttatt gaacttgaac ggggacgcga
     4561 acgcagtccc gctcatgccc ggcaacgatt tgtcggcgta ttgggcgagc gcgggcttcg
     4621 ggtcccaagt gtccacctgc gacggcgcgc cttgggcaaa aacatggatg acgtgcttgg
     4681 ccttggcggg gaatggcggg ttcttgggag cgagcggatt gaattgcgtg tcatcggcgc
     4741 gagccacgcc gccaaacatt tcagggccga gcaacgtcgc aaggcccaaa gcgcccaaac
     4801 ccatgccgaa gcggttcagg aattgacggc gtgtgatggc aaaatcctcc gggcgcgcca
     4861 catgttcatt gatattcata aatcatcaaa agtgctgctc aacgtgtgct ttcaagtcgc
     4921 tgattcgtta gacgcggttc ggagtggcgg ggtttcaata tttcaaccgc accataagct
     4981 gaaaaaggag caggttccag tccagaaccc acaactgaga agtccgccgt tcgtgacatg
     5041 agaggagttg tcatggcttg actgggtgca taaaaatggc ttgactagcc ttgactccat
     5101 ttggacaaaa tttgaagtaa gtagttgatg gaaagtgtct tgtttggatt tttgcatggc
     5161 ttgaccaagc cttgactcgc ggcacggaat ggagtgttgg agtggcggag tattggtgaa
     5221 aaggcgcatg gtttgagcgc caaccgacac gaagcgacac agcggttcgc ggacacggag
     5281 tgatggagta atgggttggg caagccttga ctcggcgcag ataatggc
//
LOCUS       DASZMM010000481         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1846383, whole genome shotgun sequence.
ACCESSION   DASZMM010000481 DASZMM010000000
VERSION     DASZMM010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846383"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1989
                     /locus_tag="VG754_11620"
     CDS             76..1989
                     /locus_tag="VG754_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HWF19912.1"
                     /translation="MNLNSNIPSGPVAQKWDKHKFDMKLVNPANKRKFDIIVVGSGLA
                     GASAAATMAELGYQVKCFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIK
                     GGDFRSREANVYRLAQVSVNIIDQCVAQGVPFAREYGGLLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALCRQIGQGTVKMFPRTEMLDVVVIDGHAKGIITRDLVTGEISAHA
                     ADAVVLATGGYGNVFYLSTNAKGCNVTATWRAYKKGAAFANPCYTQIHPTCIPVSGEH
                     QSKLTLMSESLRNDGRVWVPKKKGDTRRPDQIPEDERDYFLERKYPSFGNLAPRDISS
                     RAAKEACDDGRGVGPGGLGVYLDFADAIKRLGEETVRERYANLFDMYEKITGEDAYHT
                     PMRIYPAVHYTMGGSWVDYNLMSSLPGLFVIGEANFSDHGANRLGASALMQGLADGYF
                     ILPYTIGNYFASAKQAKPKIDHPEFKKALEGAQNLTKRLLGINGKRSVTSFHRELGKL
                     MWEDCGMARSKESLQNALKRIPELRAEFWQNLKVTGENSSLNQDLEAAGRVADFLEFA
                     ELLCLDALTREESCGGHFRVEYQTPDGEAKRDDEKFSFVSAWEYQGPDKPPLLNKEPL
                     VYEEVHMSTRSYK"
     gene            2135..>2379
                     /locus_tag="VG754_11625"
     CDS             2135..>2379
                     /locus_tag="VG754_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWF19913.1"
                     /translation="MNFTLKIWRQKKADEPGRMVEYQATGIIPDMSFLEMLDVVNEGL
                     IGKGEEPIAFDSDCREGICGTCSLVINGFAHGGQRGTT"
BASE COUNT          554 a          588 c          732 g          505 t
ORIGIN      
        1 cggctacatc tcgataccca tcgcgattca actgggttat ggacaggcat atcttgcgga
       61 aaggggattg ctgaaatgaa cctcaattca aacattccct ccggcccagt cgcgcaaaag
      121 tgggacaagc acaagttcga catgaagctg gtcaacccgg cgaacaaacg caagtttgac
      181 atcattgtgg tgggcagcgg attggctggc gcttcggcgg ctgccacgat ggcggaactt
      241 ggctatcagg tgaagtgctt ctgctttcag gacagtcccc gtcgcgcgca cagcattgcg
      301 gcgcaaggcg gcatcaatgc ggccaagaat tatcagaacg acggcgacag tgtttaccgg
      361 cttttttacg acaccatcaa gggcggcgat ttccgctccc gcgaggcaaa tgtatatcgg
      421 ctggcgcagg taagcgtgaa catcattgac caatgcgttg cgcaaggcgt tccgttcgcg
      481 cgcgaatacg gcggtttgct cgcgaaccgc tcgttcggcg gcgcgcaagt ttcgcggaca
      541 ttttacgcgc gcggtcagac cggacaacaa ttactgctcg gcgcgtatca ggcattgtgc
      601 cggcaaatag ggcaggggac ggtaaagatg tttcctcgca cggaaatgct ggacgtggtc
      661 gtcatcgacg ggcacgcgaa aggaatcatc acgcgggatt tggtgacggg cgagatttcg
      721 gcgcacgcgg cggatgcggt ggtcttggct acgggaggct acgggaacgt gttttatctt
      781 tccaccaatg ccaagggttg caacgtgacg gcgacatggc gcgcttacaa gaaaggcgcg
      841 gcgttcgcga acccgtgtta cacgcaaatc catccgacct gcattcccgt gagtggcgaa
      901 catcaatcaa agctgacgtt gatgtccgaa tcgctgcgca atgacgggcg cgtctgggtg
      961 ccgaagaaaa agggagatac gcggcgtccc gaccagattc ccgaagatga gcgcgattat
     1021 tttctggaac ggaaatatcc gagcttcggc aatcttgccc cgcgcgatat ttcatcccgc
     1081 gcagcgaagg aagcttgcga tgacgggcgc ggcgtcgggc cgggcgggct tggggtttat
     1141 ctcgattttg cggacgccat caagcgattg ggcgaggaaa ctgtgcgcga gcgttatgcc
     1201 aacttgttcg acatgtacga gaaaatcacc ggcgaagacg cgtaccacac gccgatgcgg
     1261 atttatccgg cggtgcatta tacgatgggc ggctcgtggg tggattataa cctgatgagc
     1321 agcttgccgg gattgttcgt catcggcgag gcgaactttt cggaccacgg cgcgaaccgt
     1381 ctcggcgcga gcgcattgat gcaaggcctt gcggacggtt atttcatttt gccttacacc
     1441 atcggcaatt attttgcctc ggcgaagcag gccaagccga agatcgatca tcctgaattc
     1501 aagaaggcgc tggaaggcgc gcaaaatttg acgaagcgcc tattgggcat caacggcaaa
     1561 cgaagcgtca catcgttcca ccgcgaattg ggcaaattga tgtgggaaga ttgcggcatg
     1621 gcgcggagca aggagagctt gcagaacgca ttgaagcgca tcccggaatt gcgcgcggaa
     1681 ttttggcaga acttgaaagt gaccggcgag aattcgtcgt tgaaccagga tttggaagcg
     1741 gcgggtcgcg tggctgactt tttggaattt gccgaactcc tctgcctgga cgcgttgacg
     1801 cgcgaagaat cgtgcggcgg gcatttccgc gtggagtatc aaactccaga tggcgaggcg
     1861 aaacgcgacg acgaaaagtt ttccttcgtg agcgcatggg aatatcaagg gccagacaag
     1921 ccgccgcttc tgaacaagga accgctggtt tacgaagaag tccacatgtc cacgagaagc
     1981 tacaaatgat ttgggattta cgatatgcga ttgacgcgcg cttgacgctt ttatcgatct
     2041 cattgcaatt tgtgatgagt cggatgcgaa ccttgcggtg cgcacggcgt ggaaacacgt
     2101 cgcgtaaatc atatatcgta aatcgtatat tctaatgaat tttactttaa agatttggcg
     2161 tcaaaaaaaa gccgacgagc cgggccgaat ggtcgaatat caggccacag gcatcattcc
     2221 ggacatgtca tttctagaga tgctggatgt ggtgaacgaa ggattgattg gcaagggcga
     2281 ggagccgatt gcgtttgatt cggattgccg tgaggggatt tgcgggactt gctccctggt
     2341 catcaacggc tttgcacacg gcgggcagcg cggaacaac
//
LOCUS       DASZMM010000482         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4877923, whole genome shotgun sequence.
ACCESSION   DASZMM010000482 DASZMM010000000
VERSION     DASZMM010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4877923"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VG754_11630"
     CDS             <1..1494
                     /locus_tag="VG754_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19914.1"
                     /translation="LFAWHPLHVESVAWVSERKDVLSTFFALLTLLAYACYANESKIK
                     NQKSKIYYVAALICFALSLLAKPMIVTLPFVMMLLDFWPLGRIAECGMRSAESEKSAE
                     AGATKGSTFRILQSAMEKLPFFALAALDCFATFWAQRGGQSVVSTAALPLWDRIANAL
                     VSYVLYLWKTIWPLNLALPYPYSHEWTFSTAAGAGLLLALITVVAILQARSRPWLIVG
                     WLWFLGMLVPVIGLVQVGLQAMADRYTYVPLIGIFIMVAWSIPGAWARWPRPGLVFGA
                     VTGGVLVFLLAGTETQLQYWRTSVTLFSHTAQITRDNILAEYNLGEALAQEGDLDNAI
                     IHYQKALAIRPNRVEAQYNSQPQARYNLGLIYRVQKKWKDAEAQFRDCVRDDPNLARA
                     HGNLGIALAALSRANEALQEFQTSARLNGKPLSDAQLLSTLDVAYGEAGNYSEAILTA
                     QKARDAAIAEKRPDLADAAAKRLEFYRSQTAQHPLNGSASTAVGPKQ"
     gene            complement(1568..2263)
                     /locus_tag="VG754_11635"
                     /pseudo
     CDS             complement(1568..2263)
                     /locus_tag="VG754_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
BASE COUNT          473 a          652 c          667 g          552 t
ORIGIN      
        1 ctgttcgcat ggcacccgct ccatgtcgaa tcggtggcgt gggtttcgga acgcaaggac
       61 gttttaagca ccttcttcgc tcttttgact ttgctggcct atgcgtgtta cgcgaacgaa
      121 tcaaaaatca aaaatcaaaa atccaaaata tattacgtgg ctgcgttgat ttgttttgcg
      181 ctcagcctgt tggcaaaacc gatgatcgtc acgttgccgt ttgtgatgat gttgctggat
      241 ttttggccgc tcggcagaat tgcggagtgc ggaatgcgga gtgcggaatc ggaaaaatcg
      301 gctgaagccg gggcaacaaa aggctcaaca ttccgaattc tgcaatccgc tatggaaaaa
      361 ctgccgtttt ttgcgctggc tgcgctggat tgcttcgcca cattttgggc gcaacgaggc
      421 gggcagtcag tcgtcagcac ggcggcgctt ccgctttggg accggattgc caatgcgctg
      481 gtcagttacg tgctttatct ctggaaaaca atttggccgc tgaacctcgc gcttccttat
      541 ccttattcgc atgaatggac gttttcaacg gcagccggcg caggattgct tttggcgctg
      601 ataactgtgg ttgcaatttt gcaagcgcgc agcagaccgt ggctcatcgt cggttggctc
      661 tggtttttgg gaatgctggt gccggtgatt ggattggtgc aggtcggctt gcaagcgatg
      721 gcggaccgtt acacctatgt gccgctgatc gggattttca tcatggtcgc gtggagcatt
      781 ccaggcgcat gggcgcgatg gccgcgaccg gggcttgttt ttggcgcggt gaccggcggc
      841 gtgctggtgt tcctgctggc cgggacggaa acccaattac aatactggcg caccagcgtg
      901 acgcttttca gccataccgc gcaaatcacc cgcgacaaca ttctcgctga atacaatctc
      961 ggcgaggcgc tcgcgcaaga aggcgacttg gacaatgcaa ttatccacta tcaaaaagcc
     1021 cttgcgatac gccccaatcg cgtcgaggcc caatataatt cccagcctca agcccgttac
     1081 aacctcggct tgatttaccg tgtccaaaaa aaatggaagg acgccgaggc gcaattccgc
     1141 gattgcgtgc gcgatgaccc gaaccttgcc cgcgcgcacg gcaatctggg catcgcgctc
     1201 gccgcgttgt cccgggcgaa tgaagctttg caagaattcc aaacgtccgc gcggctgaat
     1261 ggcaagcctt tgagcgacgc tcaattattg agcacgctcg acgtcgctta cggcgaagcg
     1321 ggcaattatt cggaagcaat cctgaccgcg caaaaagcgc gcgatgccgc catcgccgag
     1381 aaacgtcccg accttgccga cgcagcagct aaacgcctgg aattttaccg ctctcaaacc
     1441 gcgcagcatc cgctcaacgg atccgcttcg accgcggttg ggccgaagca gtagaaccgg
     1501 aacgccggct gccggcacgg ttgatttcac ggctcacttc ttcctgcgtg gcttccgcca
     1561 cgcgtcgggc cggtgcctca acgatgtgga atccatcgcc ttcattccat gggccgcgag
     1621 tgtcgatctc cccgtcatcg ccggtgcctg tagaatcatt gaaaaattgg ttcacgattt
     1681 ccggggtggg cgggatttcg ccgatttgca acgggtcctt gcccaagctt tccaatgccg
     1741 ccatgaaggc tttcatgtgc gtgatttccc gggtcatcag gaattgcagc gcgtccttgg
     1801 aaccggcgtc gtcgcaaaag tcgatcagcc gttcatatac gatcttcgcg cgggcttcgg
     1861 cggcgatgtt gctgcgcagg tccacgtcca gttcgccggt gatcttgaga taatccgccg
     1921 tccacggatt gcccatcgaa ttgcaaagcc cgacgcctcc cccgccggca atggcgatga
     1981 gcggatcggc ctcggcggcg gaacgctttt tcttcatcgg ctttaaatgc attttggcca
     2041 gcgagccgat gatttcaaga tggctgagtt cttcggtgcc gatgtccatc agcaaatcct
     2101 tgcgcgcggc gtcctcgcaa ttgatgcctt ggatggaata ttgcatcgcg gcggcaagct
     2161 ccccgtttgc gccgccgaat tgttccagca gcatgtttcc gaagcttggg tcgggacggt
     2221 caacgtggac ggtgtacatc agctttttga cgtggtgata catgacaatt tctttctcct
     2281 taagttgcgg ttgaatgatc aaagagccga agttccgcga ctcgcgaaaa aatctgggaa
     2341 atcg
//
LOCUS       DASZMM010000483        12525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2682479, whole genome shotgun sequence.
ACCESSION   DASZMM010000483 DASZMM010000000
VERSION     DASZMM010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2682479"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VG754_11640"
     CDS             complement(<1..1217)
                     /locus_tag="VG754_11640"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWF19915.1"
                     /translation="MYRGPAAGEQVGGGRYTLRRALGRGGMGVVWLAFDERLKEQAAL
                     KFLPPEIRLDPTALDDLRRETTQSRKLTHAHIVRIHDLYEAPNEAPFISMEYVDGPTL
                     THARMDRPTHVLEWDTLAPLIRQLCEALEYAHGEQIVHRDLKPANVMMDRRNRVKLAD
                     FGIARVLTDTMSRVTQNQRSSGTLVYMSPQQLDGKESRPIDDIYSLGATIYELLTSRP
                     PFYTGDVPHQIRYSAATSITQRLAALGICNPIPPEVEEVITACLEKEPAKRPQSAREF
                     AERLKLVDPRTPPSKRTQPATEKIPAKAPAPRPRDKTVPAILVASVLIVAGLWYGTHF
                     LRESSKGLFSTNSPPVERTNPPPKRPPDEFVSKSSPPTVGAVPNTALLATTQPKAPDE
                     IAYAPPPQKPFEPL"
     gene            complement(1326..2456)
                     /locus_tag="VG754_11645"
     CDS             complement(1326..2456)
                     /locus_tag="VG754_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF19916.1"
                     /translation="MSRVVIENLTKQFKGPKGEMICAVNRASLSVEEGEFLVLVGPSG
                     CGKSTTLRLIAGLEEITSGTVSIDGRVVNSIPPSDRDIAMVFQNFALYPHMSVHENMA
                     FGLKLRKLPRAEIEERVNRAAELLGLKDCLTRKPGELSGGQKQRVAVGRAMVRQPKVF
                     LFDEPLSNLDAQLRVQMRREIAALHSRLRATMIYVTHDQVEAMTLGNRIAVMKDGVIQ
                     QVAAPMELYRRPVNLFVAGFIGSPSMNFFRGTLCIADCGLRIAESPENAKGVNLRVPD
                     EMTARLGKFEGKQVVLGLRPEHIREQNGSTNVPSEQTISAVAEVIEPLGSETYLYAAH
                     GGNSFVARAAAEHQLHVGQTVPFVFDMSRAHFFDAASGEAIL"
     gene            2831..4054
                     /locus_tag="VG754_11650"
     CDS             2831..4054
                     /locus_tag="VG754_11650"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWF19917.1"
                     /translation="MNLDTLYSELTLKTKTKIVLLVLDGLGDIATQDQGFLTPLEAAV
                     TPNLDALARYGAQGRITPVAPGITPGSGPGHLALFGYDPLEFQVGRGVIEALGLGFDL
                     QAGDVAARANFCTLDAKGIVTDRRAGRIETAVCEELCELLSKKIKKIGATEVIIKPGK
                     GHRFVVVFRGKDLEGPLTDADPHHEGAPIPEVTPIEPGSKKAANTAELIKEFYKVALP
                     LLAGKKPANGFLMRGIAHQPHIPLFVDRYKLKPACIAVYPMYKGLAQLVGMAKLEGPQ
                     TIAEQFKRYIAEYDNYDFFFIHYKYTDMYGEDGNFDAKKKAIEELDAALPILLQKKPE
                     VIAVTGDHSTPCAAKGHSWHPQPVLLVSPLSGSDKLQRFTETGANIGSLGLFEAKYLM
                     RLMQANAKMFDKFGA"
     gene            4356..5075
                     /locus_tag="VG754_11655"
     CDS             4356..5075
                     /locus_tag="VG754_11655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWF19918.1"
                     /translation="MATLVFDIETSALPLENFDEAQQEYLFRELERIPDEIAREARRV
                     EIHRQFNLWPFTAQVVCIAMLNADTSRGQVLFTAEDYEADAAEAGPVEFVPCVDEVEL
                     LTAFWDVAQHYDLIVTFNGRGFDVPFLYLRSALLNVPVSRKNWLGYRFAIEPHCDLAE
                     QLTFYGVSGREGAARRFNLDFYCKAFGIESPKSHGVTGMDVNDLLAAGEYLKIAEYCL
                     RDVQATVLLYQIWKERLSGIK"
     gene            5101..5730
                     /locus_tag="VG754_11660"
     CDS             5101..5730
                     /locus_tag="VG754_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19919.1"
                     /translation="MNPSVLICFAVKEEAKFLPRDATARILITGMGQKNAAESLRKEL
                     SATRPGLVLSCGFAGGLNPDLKLSEIVFDSDAESGIAEKLASLGAKPVRFHCNERVAI
                     TAAEKKALRQTTGADAVEMESSALRKICREQKIPSATIRVISDTADENLPLDFNALMT
                     RDMRIDFTKLSLKLLAGPQKIPHLIRFQKQTSEAAEKLAEILEQLLAAR"
     gene            complement(5713..6147)
                     /locus_tag="VG754_11665"
     CDS             complement(5713..6147)
                     /locus_tag="VG754_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWF19920.1"
                     /translation="MLTKLLIILLIGLLFEAVGVVYLNKGLKQIGEVQKVTASEVMRI
                     VKAGATNPNILLGVLFEAIFFGTLLVLMSKGTVSFIWPLTSLGFVLTTIAAKFILHEE
                     VSWLRWCGVLLIMLGAGVITYTEKSAEGKAKVPANFESARGQ"
     gene            complement(6267..7103)
                     /gene="hpnK"
                     /locus_tag="VG754_11670"
     CDS             complement(6267..7103)
                     /gene="hpnK"
                     /locus_tag="VG754_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HWF19921.1"
                     /translation="MGRPETARRRLIVNADDFGLSSSVNQAVIRAHREGILTTASLMV
                     NEPGFEEAVALAKQNPRLGVGLHLTLSHGHSALPAKEIRGLVNERGEFSNEPIATGLR
                     YFMRREFHDALRREIFAQFEKFNATGLSLDHVNGHLHFHLHPTIFRIVMDAAEKFDAR
                     RMRLTRDPFWLNARLASGRWLYRGSHAFIYSCLSRKARGTLQRRGIRHTHKVFGLLQN
                     ALVDEAYILKLLPRLPYGDSELYSHPSLDEFKHEFDALMSERVKSAIREQGIELIRYQ
                     DL"
     gene            complement(7090..7959)
                     /gene="hpnD"
                     /locus_tag="VG754_11675"
     CDS             complement(7090..7959)
                     /gene="hpnD"
                     /locus_tag="VG754_11675"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HWF19922.1"
                     /translation="MQQSRTITQKSGSSFTPAFFFLSREKKDGMSALYAFCREVDDVA
                     DDETIPVEQRRESLAAWRADIARACRNEDPQFPVNRELQSFIQRHHLRFELFDELIKG
                     VEMDLDIKRYADYAELEQYCYRVASVVGLLSIEIFGYRNPACREYAVNLGKALQFTNI
                     LRDVRTDAERGRIYLPLSELERFKVTPEEILCGEYSSRFRELATSVAARARHFYKQAD
                     TTLPAEDRRSMLAAEMMGSVYWRLLRKLERQQFNVFGPQPTRLSKGQKILLILRTSLR
                     VTSGVMAPNYGTP"
     gene            complement(7966..8349)
                     /locus_tag="VG754_11680"
     CDS             complement(7966..8349)
                     /locus_tag="VG754_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19923.1"
                     /translation="MNHLLFFLADIDPNDILNAEQQYLNSPRLKEVLIISGTLFAAIV
                     VAAIWAFRHVKRKKRRHRYRHHHHPHSQVEGTANEQIEKSGASQESRSSKRRRRTERP
                     MNPTLAQTGGLPPIRDEKPHWPPSP"
     gene            complement(8346..9773)
                     /locus_tag="VG754_11685"
     CDS             complement(8346..9773)
                     /locus_tag="VG754_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF19924.1"
                     /translation="MSASVPPVLIVDDEKNMRLSLKAVLSDDGYSVRAAESAEEALTL
                     LGQEEFLMVITDARLGTGMSGYELLGKVHTRWPDLPILMITAYATPKLAVDAIKAGAF
                     DYLAKPFAPEELLHAVARCSERHKLLHQNAALRARANETYQLEQIVGDSQGVRELRQM
                     IQTVAPTDARVLILGESGTGKELVAGSLHCLSERRQANYIRINCAAIPETLLESELFG
                     HERGAFTGALKQKAGRVEEADGGSIFLDEIADMSRPLQAKLLRFLEDGTFTRVGGTQE
                     LRVDVRLIAATNRNIIDAIQKEEFREDLFHRLNVVQFRMPPLRERGQDVLMLAEHFLK
                     HFGQTIKKPAKKFSRAAQQKLLSHHWPGNVRELRNVIERALILETTSEIQSASLPDFQ
                     IETGLRKASAHKTVAGQSLDEMMSNFERELVTSVLEQNHFSLTRTAEQLKISRHALRY
                     RMQRLNITTEAEGEEETTAPLGKET"
     gene            complement(9781..11559)
                     /locus_tag="VG754_11690"
     CDS             complement(9781..11559)
                     /locus_tag="VG754_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF19925.1"
                     /translation="MPRFLAMPVPEPAQQASRILSIQRNIILPVRVVVTAIVFYFLFY
                     SHWLVVESMTGAVVLETLQRFFIFYILFNAVATILLLMRRFPPRFVQWVVFVVGLMDG
                     LLLGGLTLETSGFESTLFWVFPGLIMVNALSIPLATPQIVLNLSLSAFYLGAGWLYIT
                     VSESPGTIYQTPAIPHHFPARFGSSDIIDLKSLANRLAQPAEGDAVSKYLTGQFSTET
                     RLLLANAAERTNALLLRALVGDLNRIVQSGTNIYDPKRFAAVKLSSEASVLLNQRPSG
                     VDQVRLNRKLLQDAYPREISDKKHWMVSQEAKLAQQPVSEGLVVENGTEPFILRLIIL
                     WLLTASCYGVQLLLFRERLSLEEERKSVARNDELKAAGRLAAEIAHQLKNPLGIITNA
                     VYSLERGLKDGKKEFNLQMQIIREEIERSDRILTQLMGYAQLSEGRVEKLNIGDELDR
                     AVAEVFPPGATYETIVHRDYGTNLPELLMQRNHLSVVIVNLLQNAREALEERGNISVH
                     AHYGDNNTLQIVIEDDGPGISFDKLDKIFEAYVTTKQKGTGLGLAIVKHNVELYGGAV
                     RVESGLGKGARFILIFPAKTFIVTTP"
     gene            complement(11607..12158)
                     /locus_tag="VG754_11695"
     CDS             complement(11607..12158)
                     /locus_tag="VG754_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HWF19926.1"
                     /translation="MSTEKKIVLGVTGSIAAYKAAELTSLLTKQGFDVRVVMTGDAQK
                     FITPLAFKTLSRHPVMTDLYDEEEGWKPAHIELADGADLLLVAPATANVLAKLAHGLA
                     DDALSCIALALNPKAKILLAPAMNGKMWLHPATQQNVATLKSRGAEFIGPEEGLLSCG
                     YEGLGRLWPVEAVARRAGELLKS"
     gene            12291..>12525
                     /locus_tag="VG754_11700"
     CDS             12291..>12525
                     /locus_tag="VG754_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19927.1"
                     /translation="MHRNIFRFIIASFAFVAAGTLFAAERSWDFTEMTVDQPPKNCFS
                     TVAGEGKPGTWKILMDEFPLAMQPLSSSAPKTVQ"
BASE COUNT         2782 a         3487 c         3444 g         2812 t
ORIGIN      
        1 agcggttcga aaggcttctg cggtggtggt gcgtacgcga tctcgtcagg tgctttgggt
       61 tgcgtcgtgg ctagaagtgc tgtatttgga acagccccaa cagtaggcgg cgacgattta
      121 gacacaaatt catctggtgg gcgttttggc ggaggattcg tgcgctcaac cggtggcgaa
      181 ttggtggaga acaggccttt actgctttcg cgcaaaaaat gcgttccata ccacaatccc
      241 gcgacaatca aaaccgaggc gacgaggatt gccggcaccg ttttgtcgcg agggcgcggc
      301 gcgggagctt tggctggaat tttttcggtg gcaggctgcg ttctttttga tggcggtgtg
      361 cgcgggtcaa cgagcttgag ccgctcggca aattcgcgcg cgctctgcgg gcgtttggcg
      421 ggttcttttt cgaggcacgc ggtgatgact tcttcaacct ccggcggaat gggattgcaa
      481 atgcccaatg cggcgagacg ttgtgtaatt gaagtcgccg ctgaataacg gatttgatgc
      541 ggcacgtcac cggtataaaa aggcgggcgg ctggtcagca gttcgtagat ggtcgcgccg
      601 agggaataaa tatcgtcaat cggccgggat tccttgccgt ccaattgctg gggactcatg
      661 taaacgagcg tgccgctgga acgctggttt tgcgtgacgc gcgacatggt gtccgtcaag
      721 acgcgcgcga tgccaaagtc ggcaagcttg acgcggttgc ggcggtccat catgacgttg
      781 gcgggcttga ggtcgcgatg aacgatttgt tcgccatggg catattcgag cgcttcgcac
      841 aattgccgga tgagcggcgc gagcgtgtcc cattcgagaa cgtgggtggg gcggtccatg
      901 cgcgcgtgcg tcaatgtggg gccatccaca tattccatgg aaatgaaagg cgcttcgttg
      961 ggcgcttcgt agagatcgtg gatgcggacg atatgcgcgt gggtcaactt gcggctttgg
     1021 gttgtttcac ggcggaggtc gtcaagggcg gtcgggtcga gacggatttc gggcggaaga
     1081 aatttcagcg ctgcctgttc tttcagccgt tcatcgaagg ccagccagac aacgcccatg
     1141 ccgccgcggc caagcgccct tcgaagggtg taacgtcccc cgcccacctg ctcgccagca
     1201 gcgggacccc ggtacatggg tgaatcaaat tctccggttt caggagcacg catacctcat
     1261 accttctgaa ggcccggagc ttacaacacc cgcccaatgc ttgcaagcag gggaattgaa
     1321 ctttgctata agattgcctc tcccgacgct gcgtcaaaaa aatgcgcccg gctcatgtcg
     1381 aacacaaagg gaacggtttg cccaacatgt aattgatgct ccgccgcagc gcgggcgacg
     1441 aacgagtttc ccccatgggc ggcataaaga tacgtttcgg atcccaacgg ctcaatcacc
     1501 tctgcgactg cgcttatagt ttgttcagag gggacattcg ttgatccgtt ttgttctcgg
     1561 atatgttccg gccgcaagcc aagcacaact tgtttgcctt caaatttccc caaccttgcg
     1621 gtcatttcat ccgggacacg caaattgaca cccttcgcat tttccggtga ttccgcaatc
     1681 cgcaatccgc aatccgcaat acagagcgtc ccgcgaaaaa aattcatcga aggcgagcct
     1741 atgaacccgg cgacgaacag atttaccggg cgacgataca attccatcgg tgcggccact
     1801 tgctggatga cgccgtcctt catcacggca atgcgattgc ccaacgtcat cgcctcgacc
     1861 tggtcgtgcg tcacgtaaat catcgtcgcg cggagacgcg aatgcagcgc ggcaatctcg
     1921 cggcgcatct ggacgcgcaa ttgggcatcc aggttcgaca acggttcgtc gaacaaaaac
     1981 actttcggct gacgcaccat cgcgcggccc actgcgacgc gctgtttttg tccgccggaa
     2041 agttcgcccg gtttgcgcgt gaggcaatct ttcaacccaa gcaattccgc ggcgcggttg
     2101 acccgttcct cgatttccgc acgcggcaat ttccgcagct tcagcccgaa cgccatgttt
     2161 tcatggacgc tcatgtgcgg atagagcgcg aaattttgaa acaccatggc gatgtcgcgg
     2221 tccgaaggcg gaatggaatt gaccacgcgc ccatcaatgg aaactgtgcc ggatgtgatt
     2281 tcctccaagc ccgcaatcag gcgcaaggtg gtggacttgc cgcaacccga aggcccgaca
     2341 agcacgagga attcgccttc ctccacgctc aggcttgccc gattgaccgc gcaaatcatt
     2401 tcacccttgg ggcccttgaa ttgcttggtc aggttctcga tgacgacacg actcataatg
     2461 acaaaatgac taatgacgaa atacccaagg aaccgcgaac ctccaagctt caaatttagg
     2521 gaacgcaaaa tgtgacatca ttacaagcta tctcataaag tggagcgcgg ctgtgcgcga
     2581 agcgccagcc gcagcaaacc atcgaatctt gggtcttcca cgctgctgcg accggtcgtg
     2641 cggacacagt cgcgttccgt tctccattga tgagacgact tgtagaaatt taaaatggtg
     2701 cttggaattt cgtgcttctt tgggttcttg gttatttcgt agtcaggtat ttcacgttga
     2761 cgcttccctc gcattcccga atattatttc acaattcgtg tgcaacgaat ttggcaaatg
     2821 aactttgatc atgaatctcg acactctcta ttctgaactc acactgaaaa ccaaaaccaa
     2881 gatcgtcctg cttgtgctgg acggcttggg cgacatcgcc acgcaggacc aaggttttct
     2941 gactccgctt gaagccgcgg tcactcctaa cctggacgcg ctcgcacgat atggtgcgca
     3001 aggccgcatc acgccggtcg cgcccggcat cactcctggt agcggtcccg gccatctcgc
     3061 gttgtttggt tacgacccgc ttgaatttca ggtcgggcgc ggcgtgattg aggcgcttgg
     3121 tcttggtttt gatttgcagg ctggcgatgt cgccgcgcgc gcaaatttct gcacgctcga
     3181 cgcaaagggc atcgtgacgg atcgccgcgc ggggcgcatt gaaacggcgg tgtgcgaaga
     3241 actctgcgaa ttactctcca agaaaataaa aaaaatcggc gccacagaag tcatcatcaa
     3301 gcctggcaaa ggccatcgtt tcgtggtggt gtttcgcgga aaagatttgg aaggcccgtt
     3361 gaccgatgcc gatccgcatc acgaaggcgc gccgattcct gaagtgacgc caattgaacc
     3421 gggctcaaaa aaagccgcta acaccgccga gttgatcaag gagttttaca aggttgcgtt
     3481 gccgctgctc gccggcaaga agcctgcgaa tggttttctc atgcgcggca ttgcgcatca
     3541 gccgcacatt ccgctttttg tggaccgtta caaactgaag cccgcgtgca tcgccgttta
     3601 tccgatgtat aaagggctgg cgcaactggt cggcatggcg aaactcgaag gcccgcaaac
     3661 catcgccgag cagtttaagc gttacatcgc cgaatacgac aactacgatt tcttcttcat
     3721 tcattacaaa tacaccgaca tgtacggaga ggacgggaac tttgacgcga agaaaaaagc
     3781 catcgaagaa ctggacgccg cgctgccgat tttgctgcaa aaaaaaccgg aagtcatcgc
     3841 ggtcaccggc gatcactcca cgccgtgcgc ggcgaaggga cattcgtggc atccgcaacc
     3901 ggtgctgctc gtttcgccgt tgtccggttc ggacaagctc cagcgtttca cggagaccgg
     3961 cgcgaacatc ggctcgctcg gtttgttcga ggcgaagtat ctcatgcgcc tcatgcaggc
     4021 gaacgcgaag atgttcgaca agttcggcgc gtgaaatttt ttgagaaaat tctggtcagc
     4081 ggcgaaattt ctggcttaat cgttttcagt gatccaggaa caccaattga tcgggggtga
     4141 tttccatggc agccaagaag aaggctaaaa aaaagaagaa ataactttca tcgggtgaac
     4201 gaacggcaat gcaaagatgt atcgtgatgc cgttcactca cctcaaccct ctccccccca
     4261 atgggggaga gggagacgca acaaacatcg ccacccatcc cggcgatgcc cgtcctccgg
     4321 gtcgcttcca atcacatttc gccgccctcc acgccatggc cacactggtt tttgacatcg
     4381 aaacttccgc gctgccgctc gaaaattttg acgaggcgca gcaggaatat cttttccgcg
     4441 agctggaaag aattcctgac gaaattgcgc gcgaggcccg ccgtgtggaa atccatcggc
     4501 agttcaatct atggcctttc accgcgcagg tggtctgcat cgcgatgctc aacgccgaca
     4561 cttcgcgcgg ccaggtgctt ttcaccgccg aggattatga ggctgatgcc gccgaagccg
     4621 gcccggtcga gtttgtcccg tgtgtcgatg aagtcgaact gctcacggcg ttctgggacg
     4681 tcgcgcaaca ttacgacttg attgtgacat tcaatggacg cggtttcgac gtgccatttt
     4741 tatatctgcg ttcagcgctg ttgaacgtgc cggtctcgcg caagaactgg cttggttatc
     4801 gtttcgcgat cgaaccgcat tgcgacctgg ccgaacaact gactttttat ggcgtcagcg
     4861 gacgcgaagg cgctgcgcgg cggttcaatc ttgattttta ttgcaaggcc ttcggcattg
     4921 aatcgcccaa aagccacggc gtcaccggca tggacgtgaa cgacctgctt gccgccggtg
     4981 aatacctcaa aatcgcggaa tattgcttgc gcgacgtgca agcgaccgtg ttgctctacc
     5041 agatttggaa ggagcggctg tcggggatca aatgatgcat cgcgacgatc cgatcaccca
     5101 atgaacccat cggtcctcat ttgttttgcc gtgaaggaag aggcaaagtt tctgccccga
     5161 gacgcgaccg cacgaatttt gatcaccggc atgggacaaa aaaatgcagc agaaagtttg
     5221 cgcaaggaat tgtccgcgac gcggcccggg ttggttctca gttgcggatt tgcgggagga
     5281 ctgaatccgg atttgaagct gtcagagatc gtgtttgatt cggacgcgga atcggggatt
     5341 gctgaaaaat tggcttcgct tggcgcaaag ccggtgcggt ttcattgtaa cgaacgtgtt
     5401 gcgatcaccg ctgccgaaaa aaaagcgttg cggcaaacca ccggcgcgga cgcggtcgag
     5461 atggaatcct ctgccctccg aaaaatctgc cgggaacaaa agattcccag cgccaccatc
     5521 cgtgtgattt cggacacggc cgatgaaaat ctgccattgg atttcaatgc gctgatgacg
     5581 cgagacatgc ggatagactt taccaagctc agcttaaaac tgctggccgg cccgcaaaaa
     5641 attccacacc tgatacggtt ccaaaagcag acttctgaag ccgcggagaa gttggcggaa
     5701 attttggagc agctattggc cgcgcgctga ttcaaagttc gcggggactt tcgcctttcc
     5761 ttcggccgat ttctctgtgt aggtgatgac acccgcaccg agcatgatga gcagcacgcc
     5821 gcaccaacgg agccatgaga cttcctcgtg caaaataaat ttggccgcaa tggtggtcag
     5881 cacgaaaccg agcgacgtca aaggccagat gaagctgact gtgcctttgg acatcaacac
     5941 caatagtgtg ccaaagaaaa tcgcctcgaa caaaacaccc agcaaaatat tggggttcgt
     6001 cgcgcccgcc ttgacaatgc gcatcacttc ggaggcggtg accttttgga cttcgccgat
     6061 ctgtttcaat cccttgttga gataaaccac gcccaccgct tcaaagagca ggccgatgag
     6121 caaaataatt aatagtttgg tcaacatggc cactgattaa acacggattg aacacggatg
     6181 ccagacacga attgcacgaa tattcacgga tgtacagggc ccgttcgcaa ttttttataa
     6241 ttcgtgaaat tcgtgtcacg ttcccttcac aaatcctgat aacgaatcag ttcgatcccc
     6301 tgttcacgaa tggccgattt cacgcgctca ctcatcaagg cgtcgaattc gtgcttaaat
     6361 tcgtcgagtg acgggtgcga gtaaagctcg gaatctccat acggcaatct tggaagcaac
     6421 tttaaaatat aggcttcgtc caccagcgca ttttgcagca atccgaaaac cttatgcgtg
     6481 tggcggatgc cgcgccgttg cagcgttccc cgcgcctttc gagagaggca actgtaaata
     6541 aatgcgtgag agccgcgata caaccaacga cccgacgcaa gacgcgcgtt caaccaaaac
     6601 gggtcgcgcg tcaatcgcat ccggcgcgcg tcaaactttt ccgcagcatc catcacaatg
     6661 cgaaagatgg tggggtgcaa atgaaaatgg agatggccat tgacgtggtc gagcgaaagt
     6721 cctgtcgcgt tgaatttttc aaattgcgca aagatttccc gtcgcaaagc gtcgtggaat
     6781 tcgcggcgca tgaaataacg caagcctgtg gcgatcggtt cattgctgaa ttctccgcgc
     6841 tcattgacga ggccgcggat ttccttggcg ggcagcgcgg aatgaccatg cgaaagcgtg
     6901 agatgcaacc cgacgccaag ccgcggattt tgtttcgcaa gcgcaaccgc ttcttcgaaa
     6961 cccggctcat tgaccatcaa gctggccgtg gtgagaatgc cttcgcgatg cgcgcggatg
     7021 accgcttggt tgacgctgga tgaaaggccg aaatcatccg cgttgacgat gagccggcga
     7081 cgcgcagttt cagggcgtcc cataattcgg cgccatgacg ccggaagtca cgcgcaagct
     7141 ggtgcggaga atgaggagga ttttctgtcc cttgctcaac cgcgtcggtt gagggccgaa
     7201 aacgttgaac tgctgtcgct ccagcttccg caagaggcgc cagtacaccg agcccatcat
     7261 ttccgcggcg agcattgagc gcctgtcctc ggctggcaat gtggtgtccg cttgtttgta
     7321 gaaatgacgg gcgcgcgcgg ccacgctggt ggcaagttcc cggaagcgtg aagaatattc
     7381 gccgcacaaa atttcctcag gcgtgacttt gaaccgctca agttcggaaa gcggcaggta
     7441 aatccgcccg cgctcggcgt ctgtgcgcac gtcgcgcaag atatttgtga attgcagggc
     7501 cttgccaaga ttcacggcgt attcacggca ggcaggattg cgatagccaa aaatttctat
     7561 gctgagcagc ccgaccaccg atgccacgcg gtaacaatat tgttccagtt ccgcgtaatc
     7621 agcgtaacgt ttgatgtcca gatccatttc cacgcccttg atcaactcgt cgaataattc
     7681 gaaacgtaga tgatggcgtt ggatgaagga ttgcagttcg cggttgaccg gaaattgcgg
     7741 gtcctcgttc cggcaggcgc gagcaatgtc cgctcgccat gcggcgagac tttcgcggcg
     7801 ttgctcgacc ggtatggttt cgtcatcggc cacgtcgtcc acctcgcggc agaaggcata
     7861 gagcgcggac atgccgtcct tcttttcacg cgagaggaaa aaaaaggccg gcgtgaaact
     7921 ggaaccgctc ttttgagtga ttgtccggct ttgttgcatg acggctcaag gcgaaggcgg
     7981 ccagtgtggt ttttcatccc ggatgggcgg caggccaccc gtttgcgcga gagtggggtt
     8041 catcggacgt tcagtccggc gacggcgttt actgctccga ctttcctgtg atgccccgga
     8101 tttttcgatt tgctcattgg ccgttccttc cacctgtgaa tggggatggt gatgatggcg
     8161 atatcggtgc cggcgctttt ttctttttac gtgcctaaaa gcccagatcg cagccaccac
     8221 gatggccgca aacaatgtgc cagaaatgat gaggacctct ttgagccgcg gtgaattgag
     8281 gtattgctgc tccgcgttga gaatgtcgtt gggatcaatg tccgccagga aaaagagcag
     8341 gtggttcatg tctcctttcc gagcggcgcg gtggtttcct cctcgccttc ggcttcggtg
     8401 gtgatgttca aacgttgcat ccggtaccgt aaagcatggc ggctgatttt caattgctcg
     8461 gcagtgcggg tgaggctgaa atggttttgt tccaggacgc tggtcacgag ttcacgctca
     8521 aaattggaca tcatctcgtc caacgattgc cctgcaacgg tcttgtgggc ggaagccttg
     8581 cgaaggccgg tttcaatctg gaagtcaggc aggcttgccg actggatttc cgacgtggtc
     8641 tcaaggatca gcgcccgttc gatgacattg cgcaattccc gcacgttgcc cggccaatgg
     8701 tgcgacaaca atttctgctg ggcggcgcgg gagaatttct tggccggttt cttgatggtc
     8761 tggccgaaat gtttgagaaa atgttcggcg agcatcagca cgtcctgtcc acgctcgcgc
     8821 aatggcggca tgcggaattg caccacgttc agccggtgaa acaaatcttc gcggaattct
     8881 tccttctgga tcgcgtcgat gatgttccgg ttcgtcgcgg cgatgagccg cacgtccacg
     8941 cgtaattcct gcgttccgcc gacgcgcgtg aacgtgccgt cctcaaggaa gcgcaacagc
     9001 ttcgcctgca gcggacggct catgtccgcg atctcatcca ggaaaatcga gccgccatcg
     9061 gcttcttcca cgcgaccggc cttttgtttg agcgcgcccg tgaaagcgcc gcgttcatga
     9121 ccgaaaagtt cgctctcaag caacgtctcg ggaatggcgg cgcaattgat tcggatataa
     9181 tttgcctgcc gtcgttcact gagacaatgc aacgagcctg cgacgagttc cttgccggtg
     9241 ccgctctcgc caaggatcaa aacgcgcgca tcggtcggcg cgacggtttg gatcatctgc
     9301 cgcaattcgc gcacgccttg ggaatcgccc acgatctgtt cgagttgata ggtttcgttc
     9361 gcgcgcgcgc gcaaagcggc attttgatgc agcagcttgt ggcgttccga acagcgcgcg
     9421 actgcgtgca acaattcttc cggcgcaaag ggctttgcca gataatcgaa cgcgcccgcc
     9481 ttgatggcat caacggccaa tttaggcgtg gcgtaagcgg tgatcatgag gatgggcaga
     9541 tccggccagc gcgtgtggac cttgcccaac aattcgtagc cgctcattcc ggtgcccaag
     9601 cgagcatccg tgatgaccat caggaactct tcctgcccca aaagcgtgag cgcttcctcg
     9661 gccgattcgg cggcgcggac agaatagccg tcatcggaaa gcacggcctt caacgaaaga
     9721 cgcatgttct tttcgtcgtc aacaatcaac acaggtggca ctgaggcact catcaatctt
     9781 ttaaggcgtt gtcacaataa aggtctttgc agggaatatt agtatgaaac gagcgccttt
     9841 tccaagccca gattcaaccc tcaccgcgcc gccatataat tcgacgttat gcttcacaat
     9901 tgccagtccc agaccagttc ccttctgctt ggtggtgaca taagcttcaa aaatcttgtc
     9961 cagcttgtca aacgagatgc caggcccgtc atcttcaatg acgatttgca gcgtattatt
    10021 gtcgccgtaa tgcgcatgga cagaaatatt tccgcgttct tccagggctt cgcgcgcgtt
    10081 ttgcagcagg ttcacaatca cgacggacaa atggttgcgc tgcatgagca actcaggcag
    10141 attagtgccg tagtcccggt gtacgattgt ctcgtaagtc gcgcccggcg gaaacacttc
    10201 ggcaaccgcc cggtccagtt catcaccgat gttcaatttc tcgacacgcc cttcgctcaa
    10261 ttgcgcgtat cccatcaatt gagtcaaaat ccggtcggaa cgctcaatct cttcgcggat
    10321 gatttgcatc tgaaggttga attccttctt gccgtccttc aaacctctct ccagcgaata
    10381 gaccgcgttg gtgatgatgc caagcgggtt tttgagctgg tgcgcgattt ctgcggcgag
    10441 gcgtcccgct gcttttaatt catcattgcg cgccacagat tttcgttctt cctccaatga
    10501 aagccgctcg cggaacaaca acaattgcac cccgtaacaa ctcgccgtca acagccacaa
    10561 aatgatcagc cgcaaaataa acggctccgt tccattttcc accaccaatc cctcagacac
    10621 cggttgctgc gccagtttgg cctcttgcga aaccatccaa tgttttttgt ccgaaatttc
    10681 ccgcgggtag gcatcctgca gcagtttccg gttcaatcgc acctggtcca ctccggacgg
    10741 tctctggttc aacaagaccg atgcttcgga ggaaagtttg accgcggcaa aacgcttcgg
    10801 atcataaata ttcgtcccgc tctgcacgat gcggtttagg tccccgacca gtgctcgcag
    10861 cagcaaggcg ttggtccttt cagcggcgtt ggccagcaga agacgcgttt ctgtcgaaaa
    10921 ctgtccggtc aaatatttcg atacggcatc tccttcggca ggttgcgcga gacggttcgc
    10981 cagcgatttc aaatcgatga tgtcgctcga cccaaatcgc gccggaaaat gatgcggaat
    11041 ggcgggcgtc tggtaaatcg tgccggggct ttcgctgacg gtgatgtaca accagccagc
    11101 cccgagataa aaggcgctga gcgaaaggtt caagacgatc tgcggcgttg ccagcggaat
    11161 gcttaatgcg ttcaccatga tcaaaccggg aaacacccaa aacaacgtgc tttcaaagcc
    11221 gcttgtttcc agcgtcaatc cgccgagcaa taaaccatcc atcaatccca ccacgaagac
    11281 cacccattgc acaaaccttg gcggaaaccg ccgcatgagc agcaggatgg tcgcaaccgc
    11341 attgaaaagg atgtagaaga tgaaaaaccg ttgcagcgtt tccagcacca ccgctcccgt
    11401 catcgattcc accaccagcc aatgcgagta aaagaggaaa tagaatacaa tggcggtcac
    11461 caccacgcgc acaggcagga tgatgttgcg ctggatggaa aggatgcgcg aggcctgctg
    11521 cgccggttcg ggcaccggca tggccaaaaa ccggggcaac cacttcggtc tttgccaatg
    11581 gatggaattc atatcgggca tcggcctcaa ctcttgagca attctccggc ccgccgtgcg
    11641 accgcctcca ccggccacaa ccggcccaat ccttcataac cgcacgaaag caatccttcc
    11701 tccggcccga tgaattccgc gccgcgcgat ttgagcgtcg cgacattctg ttgcgttgcc
    11761 ggatggagcc acatcttgcc attcatcgcc ggagcgagaa ggattttcgc cttgggattc
    11821 aatgccagcg cgatgcaact taaagcgtca tccgccaatc catgcgcaag ctttgcgaga
    11881 acattcgccg tggccggggc aacgagcagc aagtccgccc catcggccag ttcgatatgg
    11941 gctggtttcc agccttcttc ttcgtcatac aaatcggtca tcaccgggtg gcgcgagagt
    12001 gttttgaacg cgaggggcgt gatgaatttt tgcgcatcgc cggtcatcac cacccgcacg
    12061 tcgaaaccct gcttcgtcag cagacttgtc agctccgccg ctttgtaagc ggcgatggaa
    12121 cccgtcacac ccaaaactat ctttttttcg gtgctcattg cgtttttccc gacgctgcat
    12181 cataacccgc ccttttcgca ttatgcaaag agattaagtg ggcgtttgcc acgcaaattc
    12241 agccgccatt aaacttgtcg ccctgaggtt ttttgctacg ctcacccgca atgcacagga
    12301 atattttccg tttcatcatt gcgtcgttcg cgttcgtggc cgccggaact ttatttgccg
    12361 ccgaacgctc gtgggatttt acggagatga cagttgacca gccgccgaaa aattgtttca
    12421 gcactgtggc cggtgagggc aagccaggga catggaagat tctcatggat gaattcccgc
    12481 tggccatgca gccgctcagc agcagcgctc ccaagactgt ccaaa
//
LOCUS       DASZMM010000484         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1020353, whole genome shotgun sequence.
ACCESSION   DASZMM010000484 DASZMM010000000
VERSION     DASZMM010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020353"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1437
                     /gene="carA"
                     /locus_tag="VG754_11705"
     CDS             277..1437
                     /gene="carA"
                     /locus_tag="VG754_11705"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWF19928.1"
                     /translation="MKAILALEDGSVFHGAGFGAQTTACGEVCFNTSMTGYQEILTDP
                     SYKGQIVTMTYPLIGNYGVNEQDVESWRPHVAGFVIRELSPMVSNWRADLPLADYLQQ
                     NGIPGIQGIDTRALTKKLRVRGALKGFISTEGTPEAEAIERAKSWEGLAGVDYVKEVT
                     HKKAFRWDEKDEQSANFNLVRGAVATQAREARDPLPPADIPIVAFDYGMKYNILRRLR
                     QQGFAVQVVPATATAEEALKYKPAGIFLSNGPGDPAALGYAVKAAGDLMKSGIPIFGI
                     CLGHQILGQAFGGKTFKLKFGHRGANQPVKDLETGRVEITSQNHGFAVDAKSLPSDVV
                     VNRLNLNDQTVEGMRHKTKPIFCVQYHPEASPGPHDSTPLFAEFREMLMKKV"
     gene            1519..2058
                     /locus_tag="VG754_11710"
     CDS             1519..2058
                     /locus_tag="VG754_11710"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWF19929.1"
                     /translation="MAKLVVLSEGLTGQSYELKVDKTTIGRVEDNTFQIAQPSVSSHH
                     CEILLKGSDVVVKDLNSTNGTFINNQQIAGEAVLKVGQILRLGQVDIRLESGQAPAAG
                     TPASGKRLVDSTMVIAKGVSLEQLEQGPHGLDTTKGVFTKKKNDTNKWFIIGGVVLLA
                     VIVIAILYSLNLVSGHSSH"
     gene            complement(2063..>2449)
                     /locus_tag="VG754_11715"
     CDS             complement(2063..>2449)
                     /locus_tag="VG754_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWF19930.1"
                     /translation="SAYYGPFREAAESTPQFGDRRSYQMDAGNANEALREVAMDIQEG
                     ADMIMVKPALAYLDIIHRVKTEFGYPTAAYAVSAEYSMIKAAADKGWIDERAVTLESL
                     LAMRRAGADVLITYHATDVARWLKAQ"
BASE COUNT          564 a          656 c          695 g          534 t
ORIGIN      
        1 ccgagggtgt tcgcggcgga ggttcgcctg aagtcgtcgt tcgggacagc gacggcaaac
       61 ttttaaatct ctctttggcc cggtgagaag caaacaagcc gtcatatccg gctccgttca
      121 aataattcca gagagtgcaa actttttggt ttggtcattg ttatttgaat tttctctgga
      181 gcttggatgt tggcgcttgg agcttttttg gggcaaacgg catggacata ataccagctt
      241 cagtttagga ttcgcccgaa gttgagttga cgaatgatga aagcaatatt ggcgttggaa
      301 gatggttcgg tgtttcacgg agcaggtttc ggcgcgcaga caacggcctg cggcgaagtt
      361 tgtttcaaca cttccatgac cggttaccag gaaatcctca ccgacccttc ctacaaaggc
      421 cagatcgtga cgatgactta cccgctcatc ggcaattacg gcgtgaacga acaggacgtc
      481 gaatcgtggc gtccgcatgt cgcggggttc gtcatccgcg aactttcgcc gatggtgagc
      541 aactggcgcg cggaccttcc attggcggat tatcttcagc aaaacggcat tcccggcatc
      601 cagggaattg acacgcgcgc gttgacgaaa aagttgcgtg tccgcggcgc gctcaaaggt
      661 ttcatttcca ccgaaggcac gcccgaagcc gaagccatcg agcgcgcgaa atcatgggaa
      721 ggtttggcgg gcgtggatta tgtgaaggaa gtcacgcata aaaaagcttt ccgctgggac
      781 gaaaaggacg agcagagcgc gaatttcaat ctcgtcagag gcgccgtcgc cacgcaagcg
      841 cgggaagcgc gcgatccact tccgccggcg gacatcccca tcgtggcttt tgattacggg
      901 atgaaataca acatcctccg gcgattgcgt cagcaaggtt ttgcggttca agtcgtgccg
      961 gcaacggcga cagcggagga agcattgaaa tataaacctg cgggaatttt tctctcgaat
     1021 ggcccgggcg atcccgcggc attgggttat gcggtcaagg cggcgggcga tttgatgaag
     1081 agcggaatac cgatttttgg gatttgcctt gggcaccaga ttttgggcca ggcgtttggc
     1141 gggaagacat tcaaattgaa attcggccat cgcggcgcga accagccggt gaaggatttg
     1201 gagactggtc gcgtggagat cacctcgcaa aaccatggtt tcgccgtgga cgcaaaatca
     1261 ttgccctccg acgtcgttgt gaaccgcctc aacctgaacg accagacggt cgaagggatg
     1321 cggcacaaga caaagccgat tttctgcgtg caatatcatc cggaagcatc gccgggcccg
     1381 cacgattcga cgcccttgtt cgctgaattt cgggaaatgc tgatgaaaaa ggtttgaagt
     1441 aaagtattgc tgccaagcgg gaaatcggtt tgacttggag gcatccgatg agataaatca
     1501 gtggcagcga accagtctat ggccaaactt gtagtgctta gtgaagggtt gacggggcag
     1561 tcgtacgagc tgaaagtgga taagaccacc atcggccggg tcgaagataa caccttccaa
     1621 atcgcgcagc cctccgtctc aagccatcat tgcgagattt tgctcaaggg cagcgacgtc
     1681 gtcgtcaagg acctcaattc caccaacggc acgttcatca acaaccagca gatcgccggc
     1741 gaggctgtgc tcaaggtggg gcaaattttg cgtctcggcc aggtggacat ccggttggaa
     1801 tccggtcaag cgcccgccgc agggacgcca gcatccggca agcgtctcgt ggattcaacc
     1861 atggtgattg ccaagggcgt ctctttggaa caactggaac aaggaccgca cgggttggac
     1921 acgaccaagg gcgtcttcac caagaaaaag aacgatacca acaaatggtt catcattggt
     1981 ggagttgttt tgcttgccgt gattgtcatc gccattctct actcactgaa cctggtcagc
     2041 gggcattcgt cgcattgagt tcttattggg ccttcaacca ccgcgcaaca tccgtcgcgt
     2101 ggtaagtgat caaaacatcc gccccggccc gacgcatcgc gagcaaactt tccagcgtca
     2161 ccgcccgttc atcaatccaa cccttgtcgg cagccgcctt gatcatcgaa tattccgcac
     2221 tcaccgcata cgccgcggtc ggataaccga actcggtttt cacgcgatga atgatgtcca
     2281 aatatgccag cgcgggcttg accatgatca tgtccgcgcc ttcctggatg tccatcgcga
     2341 cttcgcgcaa cgcctcgttc gcattgcctg cgtccatttg ataactgcgc cggtcgccaa
     2401 actgcggcgt tgattccgcc gcctcgcgga acggcccgta ataagccga
//
LOCUS       DASZMM010000485         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_510066, whole genome shotgun sequence.
ACCESSION   DASZMM010000485 DASZMM010000000
VERSION     DASZMM010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510066"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1364)
                     /locus_tag="VG754_11720"
     CDS             complement(18..1364)
                     /locus_tag="VG754_11720"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWF19931.1"
                     /translation="MEAGWHSPNVTPSLQEVHRTIAVPRHFSFWRKMLAFSGPGYLVA
                     VGYMDPGNWATDLAGGAQFGYTLLSIVMLSNLMAILLQSLSAKLGIVTGRDLAQACRD
                     HYSKPVSIALWALCEIAICACDLAEVIGSAIALNLLFKMPLLWGVCVTALDVLAVMYL
                     QNKGFRYIEALVVTLIVTIGVCFLAEIIFSHPNLVGVLGGFVPRFEIVQNKDMLYIAI
                     GILGATVMPHNLYLHSSIVQTRKYEQNPEGKTEAIKYAVLDSTLALMFALFINAAILV
                     VAAATFHNSGHQDIAEIQDAYKLLSPMLGVSLAGAIFALALLASGQNSTLTGTLAGQI
                     VMEGFLNLRLRPWVRRLITRMIAIVPTVICVALYGENGAGKLIILSQVVLSLQLSFAV
                     VPLVLFTSDRRKMGEFANPLWLKILAWLTTVIIVVLNIKYLLDWAGITAWFDKLMK"
     gene            1841..2548
                     /locus_tag="VG754_11725"
     CDS             1841..2548
                     /locus_tag="VG754_11725"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWF19932.1"
                     /translation="MKQTILLRTLACCVAGSLFAGSAIAQQSSGDTSGAGGTGNLGSA
                     GQPPPPGYSTLSQSSSSRSMRSERLSQLMGATVKDQQGNTIGQINDFVVNPMSGRIQF
                     AVISLNDQSGKLAAVPWQLVRPGADPTTCSLSVSKDKLDNAQTFDASSWPDFSQPSTS
                     QQIYSHFGMQGGRSGWGNEGGTGNQNGTGSGQNQNGTGSSGYQNQNDTGTEGYHHGTG
                     TPGTGEGTGSTSTPRNP"
     gene            complement(2608..>3316)
                     /locus_tag="VG754_11730"
     CDS             complement(2608..>3316)
                     /locus_tag="VG754_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF19933.1"
                     /translation="SVLGLKGEQLGQHPEGRAGEFVRVSVIDTGSGIDAMVLPHIFEP
                     FFTTKAVGKGTGLGLATVYGIIKQHQGWIEVESKVGIGTTLHVYVPFLKIATGAKPGD
                     AKIEEPLRGGTETILIVEDEPPVRDLVCKILKSAGYEVLQANNGPEALDVWREHKDKI
                     ELLLTDIVMPDGMTGRDLAEKLQAEKPSLKAIFTSGYSSDIVGKDFIIQDGVNFLQKP
                     YMPQKLTRAVRKCLDDR"
BASE COUNT          822 a          892 c          920 g          682 t
ORIGIN      
        1 gcggccattg gtcaacatca tttcatcagc ttatcgaacc acgcggtaat gccagcccaa
       61 tcgagcaggt atttgatatt caacacaacg ataatgacag tggtgagcca tgccaggatc
      121 ttcagccaga gcggattggc aaactcgccc atcttgcggc ggtcactggt gaacagcacc
      181 aagggcacga cggcaaagga aagttgcagg ctcaacacca cctggctgag gatgatcaat
      241 ttgcctgcgc cattttcgcc atacagcgcc acacaaatca ccgtcggcac aatggcgatc
      301 atccgcgtga taagccgccg cacccacgga cgcaatcgca ggttgagaaa gccttccatg
      361 acaatctggc cggcgagagt gccggtgaga gtcgagttct ggccggaggc gagcaacgcc
      421 agcgcaaaaa tcgcaccagc caatgacacc ccaagcatgg gactcaggag tttgtaggcg
      481 tcctggatct cagcgatatc ttgatgtccg ctgttgtgaa aagttgccgc ggcaacgacg
      541 agaatggccg cgttgatgaa cagcgcgaac atcagcgcca gcgtggaatc gagcacggca
      601 tatttgatcg cctcggtttt gccttcgggg ttttgctcgt acttgcgcgt ctggacgatg
      661 gaggagtgca gataaagatt gtgcggcatg accgttgcgc caaggatgcc gatggcaatg
      721 taaagcatgt ctttgttttg gacgatttca aatcgcggaa caaaaccgcc gagaacgcca
      781 accaggttcg ggtgtgaaaa gatgatttcc gcaagaaagc agacgccgat ggtcacgatg
      841 agcgtcacga ccagcgcttc gatgtagcgg aaacctttgt tctgaagata catcactgcc
      901 agcacgtcaa gcgcagtgac gcaaacaccc caaaggagcg gcattttgaa gagcaggttc
      961 aacgcaatgg ccgagccgat gacttcggca aggtcgcagg cgcaaatggc gatttcgcac
     1021 aaggcccaca gcgcgatgga caccggcttg gaataatggt cacgacacgc ttgggcaagg
     1081 tcgcgccccg tgacgatgcc cagcttggcg ctgagggatt gcaacaggat ggccatcagg
     1141 tttgagagca tcacgatgct gagcagcgtg taaccgaatt gcgcgccacc ggcgaggtca
     1201 gtggcccaat tgccggggtc catgtaaccg accgcgacga gatagccggg gccggaaaag
     1261 gcgagcatct tgcgccagaa actgaagtgc cttgggactg cgatggtacg atgaacctcc
     1321 tgcaagctgg gcgtcacatt aggcgaatgc cagcccgctt ccacgtgggt gggtggaagc
     1381 ggatcacttg atgaagctga accgtctgtt ttcaagccgt gcacaccata acatcgcaaa
     1441 tgtttgagtc aataaaaaaa gttagacata tctataatta aaaagttgga gaggtttggc
     1501 gcggatgtgg gaggatataa tattcactgg gggaaactac ccactatgga atttttgcaa
     1561 tttatcggtt ctgaaatatg ttgaaaacag cctgttcgtt ccttattcac taatcatggg
     1621 ttaaagggac aggatgggtg gagcgactcc ttcacggagc cgctcatttt attggcaaag
     1681 atattagcca cagatgaaca cggattcatc agtttttcac cccattgttg cgcgcagagc
     1741 tgcccgtaag ttgaaacagt gtctcgccgc gacgcgcttt ttctgccgtg tcgccttgaa
     1801 aaaaagtggg actgcaacaa acgaaagaaa ggcaacaaaa atgaaacaaa caatattatt
     1861 gcgaaccttg gcctgctgcg tggccggttc gctgttcgcg ggttcggcca tcgcccagca
     1921 atcctccggc gatacaagcg gcgcgggcgg aacgggtaat ctcggcagcg caggacaacc
     1981 gccgccaccg ggatattcca ccctttcgca aagttcttca tcgcgttcga tgcggagcga
     2041 acgattgagc cagttgatgg gcgcaacggt caaggaccag caaggcaaca ccatcggcca
     2101 aatcaatgac ttcgtcgtca atcccatgtc gggccgcatt caattcgcgg tcatctcgct
     2161 caacgatcaa tcgggcaaac tggcggctgt tccatggcag ttggtgcgtc ccggcgcgga
     2221 cccaaccact tgctcgttgt cggtcagcaa ggacaagctg gacaacgcgc aaacctttga
     2281 cgcaagcagt tggccggatt tcagccagcc ttcaacgagc cagcaaatct attcgcattt
     2341 cggcatgcag ggaggccgct cgggatgggg aaatgaaggc ggcacgggaa accagaacgg
     2401 gaccggctcc ggccaaaatc aaaatggcac gggttccagc ggatatcaaa accagaacga
     2461 caccggcacg gaaggatacc accacgggac aggcacgcca gggacaggag agggaactgg
     2521 cagcaccagc actccgcgta atccttgatc gtcagggcca ctgttgcctg aatggggcca
     2581 aaccggtcca aacccggttc ggccaggtca gcgatcatca agacattttc gcacggcgcg
     2641 ggtcaacttt tggggcatgt acggcttctg gaggaaatta accccgtctt gaataatgaa
     2701 atccttgccg acgatgtccg aactgtagcc gctggtaaag atggctttga gcgacggttt
     2761 ttccgcctgc aatttttccg cgaggtcgcg cccggtcatc ccgtcgggca tgacgatgtc
     2821 tgtcagcaac agttcgattt tgtctttgtg ttcccgccag acatcgaggg cttcaggacc
     2881 attgttcgct tgcaatactt cataaccggc cgatttcaaa attttgcaga ccaaatcccg
     2941 cacgggcggt tcatcctcga cgatcaaaat tgtttcagtt ccgccgcgca aaggttcttc
     3001 aatctttgca tcgccaggtt tcgcacccgt cgcaattttg aggaacggca cgtaaacgtg
     3061 gagtgtcgtg ccgattccca ccttgctttc cacttcgatc cagccttgat gctgtttgat
     3121 gatgccatag acagtcgcca agccgagccc ggtccctttt ccaaccgcct tggtggtgaa
     3181 aaaaggctca aaaatgtgcg gcaaaaccat cgcgtcaatg ccggaacccg tatcaatgac
     3241 gctcactcga acgaattcac cggcgcggcc ttcgggatgt tgccccaatt gctcaccctt
     3301 gaggccgagg actgaa
//
LOCUS       DASZMM010000486         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_203613, whole genome shotgun sequence.
ACCESSION   DASZMM010000486 DASZMM010000000
VERSION     DASZMM010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203613"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="cmk"
                     /locus_tag="VG754_11735"
     CDS             <1..627
                     /gene="cmk"
                     /locus_tag="VG754_11735"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWF19934.1"
                     /translation="VIAIDGTSASGKSTNAKLVARALGFIYVDTGAMYRTLAWHCLQK
                     RVDPHDEKAVAALCRKWKTTLECVSNEVHLLVDGYYPAREIRTAETSAAVPHVAAVPK
                     VRDWMKKKQRECIKFGNLVMEGRDIGTNVFPETDFKFYLDATLDERSKRRAAEGVQEN
                     LAARDQRDSQRASAPLMIALGAKVINNSHMTTEQTSSLIIEEVRKRPQ"
     gene            677..1090
                     /locus_tag="VG754_11740"
     CDS             677..1090
                     /locus_tag="VG754_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWF19935.1"
                     /translation="MPIHHAQVLTCLKLSKRRLALLINFNVPLIKDGIKERISLFCTF
                     LGSCELCSWVGAHRCPPHPSPLPKERGTIWPRSINRTLSVCNHDGMRCSLSPRERAGV
                     RGRKRSITPSCRKLRWARHQTDCFMISWCLGVLVV"
     gene            1094..1726
                     /locus_tag="VG754_11745"
     CDS             1094..1726
                     /locus_tag="VG754_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF19936.1"
                     /translation="MNPVYFAGWSLYRAAFATYFRWRVYDSENVPLTGPVILAANHES
                     FLDPPLVGSSLKRDINYLARKSLFRYPVLGWVLRTVNAVPVDRDGGGAAGLRAIMDRL
                     HDGGAIILFPEGTRTSDGKLQPARSGIGLTVIKSDAPVVPVKVFGTYEAWGRHVRFPK
                     PHRVAVKYGKPMDFATLRAEAKTCSKARLKEIYQEVADEIMAAIGELKIG"
     gene            complement(1820..3109)
                     /locus_tag="VG754_11750"
     CDS             complement(1820..3109)
                     /locus_tag="VG754_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF19937.1"
                     /translation="MKHPLNRRQFLKQTGLAGASLAALGSVNSIRAAESPSRKILVGI
                     MGVNGRGMAHVAGYLAQKDCEIAYICDVDSRAMDHAISVVAKKQSTKPQGVKDFRTIL
                     DDKRVDALSIAAPNHWHAPATILACAADKHVYVEKPCCHNPHEGELMVAAARKHKRVV
                     QMGNQRRSWPWVIEAMNRLKAGEIGKVCFARTWYNNSRASIGHGNPVPVPEWLDWSLW
                     QGPAPEHAYLDNVVHYKWHWRWLWGNGELGNNGIHALDLARWGLGVDYPTRVTCSGGR
                     YHYQDDQETPDTYVTTFDFGNCGATWDGQSCDPHGFEGQGFGCTFYGEKGTLIIAGNN
                     YKIVDPKDNVLAEVKGEWDDKIHFGNFLDAIREEKQLNSEIAEGAKSTLLCHLGNIAY
                     RTGRTINFDAKTQKIADDHAAMRYWKREYRKGWEPRV"
     gene            complement(3142..3474)
                     /locus_tag="VG754_11755"
     CDS             complement(3142..3474)
                     /locus_tag="VG754_11755"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HWF19938.1"
                     /translation="MIEETLEKIEARLQHTDAISEEKRRELHDLIAKLRAEVAELSKT
                     HAEQAESIAGFTERSAHEATREQQNPRLLELSLQGLGSSVEEFERSHPKLVQIVNSIS
                     QTLSNLGI"
     gene            complement(3520..>3842)
                     /locus_tag="VG754_11760"
     CDS             complement(3520..>3842)
                     /locus_tag="VG754_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWF19939.1"
                     /translation="RGLDLKLVKAVIDFAVGETRPAITLLFQVPLAVREARLASRQAT
                     LPFMRDRIEEGDPVFFERVRKGYQVIAQEEPKRVQAIDASGTVEAVSAEVWKRIEPLL
                     PQRG"
BASE COUNT          816 a         1115 c         1033 g          878 t
ORIGIN      
        1 gtcatcgcga ttgacggcac cagcgccagc ggcaaaagca ccaacgccaa gctcgtcgcc
       61 agggcgctcg gctttatcta tgtcgatacc ggcgcgatgt atcgcacgct cgcatggcat
      121 tgcttgcaaa aaagagtgga tccgcatgac gaaaaagccg tcgccgcgct ttgccgcaaa
      181 tggaagacca cgcttgaatg cgtcagcaac gaggtccatc tgctggtgga cggctattat
      241 cccgcgaggg aaatccgcac cgccgaaacc agcgccgccg tgccgcacgt cgccgcagtt
      301 cccaaggtgc gcgattggat gaagaaaaag cagcgcgaat gcatcaagtt cggcaatctc
      361 gtgatggagg gccgcgacat cgggacgaat gtctttcctg aaaccgattt caagttttat
      421 ctcgatgcca cgctcgatga gcgttccaag cgccgcgctg cggaaggcgt tcaggaaaat
      481 ctcgccgcgc gcgatcaacg cgacagccag cgcgccagcg ccccgctgat gattgccctt
      541 ggagcaaagg tcatcaacaa ttcccacatg accaccgagc agaccagcag cctcatcatc
      601 gaggaagtgc gcaagcggcc tcaataaaaa attgaaccac caaggcacca agtcaccaag
      661 ggagacggaa aaaatcatgc caatccatca cgcacaagtt ttgacttgct tgaaactttc
      721 caaacgaagg ctggcgctgt tgatcaactt taatgtccct ctgatcaaag atggcatcaa
      781 agagcgcatc tcactatttt gcacgtttct gggctcttgc gaattgtgtt catgggtcgg
      841 cgcacatcgc tgtccccctc accccagccc tctccccaag gagaggggga ccatttggcc
      901 gcgctcgata aatcgaacgc tctcggtttg caaccacgac gggatgcgtt gctccctctc
      961 cccaagggag agggctgggg tgagggggag gaaacgttca attactccat cgtgcaggaa
     1021 actgagatgg gcccggcatc aaacggattg ctttatgatt tcctggtgtc ttggtgtctt
     1081 ggtggtctaa ttcatgaacc cggtttattt tgctggctgg agcctttatc gcgcggcatt
     1141 cgcaacttat tttcgctggc gcgtgtatga ctctgaaaac gtcccgctca ccggcccggt
     1201 catccttgct gccaatcacg aaagtttcct cgacccgccg ttggtcggtt ccagcctcaa
     1261 gcgcgacatc aattatctcg ctcgcaaatc gttgttccgc tatccggtgc tcggctgggt
     1321 gttgcgcacc gtgaacgctg tgcctgtgga tcgcgacggc ggcggcgcgg cgggactgag
     1381 agccatcatg gaccgtctgc acgatggcgg cgcgatcatt ttgtttcccg aaggcacgcg
     1441 cacgagcgac ggcaaattgc aaccggcgcg ttcaggcatc ggcttgacgg tcatcaagtc
     1501 cgatgcgcca gtcgtgccgg tgaaggtttt tggcacttac gaagcgtggg gacgtcatgt
     1561 tcgttttccc aagccgcatc gagtggccgt gaaatacggc aaaccgatgg attttgccac
     1621 acttcgcgcc gaggccaaaa cttgttccaa agcgcggctc aaggaaattt accaggaagt
     1681 cgcagatgaa atcatggcgg cgattggcga actcaagatt ggttaaaatg gttacaaggt
     1741 tgcatcgtca catggtcaaa atctgaacac acgtatcgaa ccgttgtcat tctaacctta
     1801 cgattaactc tttaacgctt cacaccctcg gttcccatcc tttgcgatac tcccgtttcc
     1861 aatagcgcat cgctgcgtga tcgtcggcaa tcttttgcgt ttttgcgtcg aaattgatgg
     1921 tgcggccggt gcggtaggcg atgtttccca agtgacaaag cagcgtgctc ttcgcgcctt
     1981 ccgcgatctc ggaattcaat tgtttttcct cgcgaatggc gtcgagaaaa tttccgaaat
     2041 ggattttgtc gtcccactcg cctttcactt ccgcgagcac gttgtccttc ggatcgacga
     2101 ttttgtaatt gttcccggcg atgatgagcg tccccttttc tccgtaaaaa gtgcaaccga
     2161 aaccctggcc ttcaaaacca tgcgggtcgc aactttgccc gtcccatgtc gcgccgcaat
     2221 ttccaaaatc aaacgtggtc acgtatgtgt cgggcgtttc ctgatcatcc tgataatgat
     2281 aacgcccgcc gctgcaagtc acgcgagtcg ggtaatccac tcccaaaccc cagcgcgcga
     2341 gatcaagtgc atggatgccg ttgttgccga gttcgccgtt gccccaaagc cagcgccaat
     2401 gccatttgta atggacgaca ttgtcgagat aagcatgttc cggcgcgggg ccctgccaga
     2461 gcgaccaatc gagccattcg ggaaccggca cagggttgcc atgaccgatg gaagcgcggc
     2521 tgttgttata ccaagtgcgc gcgaaacaaa ccttgccaat ctcgcccgcc ttcaatcggt
     2581 tcatggcctc gatgacccac ggccaactgc ggcgttgatt gcccatctgc accacgcgct
     2641 tgtgctttct tgccgcggca accatcaact cgccttcatg cggattatgg cagcacggtt
     2701 tctcaacata aacgtgcttg tccgcggcgc aggcaagaat ggtcgccggc gcgtgccaat
     2761 gattcggcgc ggcgatggac aacgcatcca cgcgcttgtc gtccaaaatt gtccgaaaat
     2821 ctttcactcc ttgcggcttg gtggattgct tttttgcgac aacggagatg gcgtggtcca
     2881 tcgcgcgcga atcaacgtca cagatataag cgatctcgca atctttttgc gcgaggtaac
     2941 cggcgacatg cgccatgccg cggccgttga cgcccatgat gccgacgagg atttttcgcg
     3001 aagggctttc cgcggcgcga atggaattga cgctgccgag cgccgcaagg cttgcgcctg
     3061 ccaggccggt ttgttttaaa aattgccgtc gattgagtgg atgcttcatg tgaggtttgt
     3121 ctttctgaat agctggattt gctaaatgcc gaggttggaa agcgtctggc tgatggagtt
     3181 cacgatctgc accagtttcg gatgcgagcg ctcaaattct tcgaccgagg aaccaagccc
     3241 ttgcagcgaa agttccagca agcgcggatt ttgttgttcg cgtgtcgctt cgtgcgcgga
     3301 acgctcggtg aacccggcga tgctctcggc ctgttcggcg tgcgttttgg acaactcggc
     3361 cacttcagcc ctcagcttcg cgatcaagtc gtgcagctcg cggcgttttt cctcggagat
     3421 tgcgtccgtg tgctggagcc gcgcttcaat tttttccagt gtttcctcga tcatcttggg
     3481 ccaccttaac ggattttgca gaaagataaa gccatgtttt catcctcgct gcggcaagag
     3541 cggctcaatg cgtttccaaa cttccgcgct cacggcttca acagttccag aggcatcaat
     3601 ggcctggacg cgtttcggtt cttcctgcgc gatgacctga taacccttgc gcacgcgctc
     3661 aaaaaaaacc ggatcgccct cctcgatgcg gtcgcgcatg aacggcaacg tggcctgccg
     3721 cgaagccaaa cgcgcttccc gcaccgcaag cggcacctgg aataaaagcg ttatcgccgg
     3781 tctcgtttcg ccgacggcaa aatcaatcac tgccttgacc agcttcaaat ccaacccgcg
     3841 cc
//
LOCUS       DASZMM010000487         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3512833, whole genome shotgun sequence.
ACCESSION   DASZMM010000487 DASZMM010000000
VERSION     DASZMM010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512833"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VG754_11765"
     CDS             complement(<1..956)
                     /locus_tag="VG754_11765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HWF19940.1"
                     /translation="MSSLLNPKRTVTELKELRALTGDENGAQRIAFTPVWTAARAWLR
                     KKLEALPVEMHMDAAGNLWTTLRGESERALLIGGHIDSVPNGGWLDGCLNVLAGVEIL
                     RRINEQYNGKPPVTVRLVDWADEEGARFGKSLFGSSACSGNLNLDEARGLKDKDGITL
                     PDALKNVGVDFERVKESGRELQNAAAYLELHIEQGPVLLDLELPLGAVLGTFGVERHA
                     ITFQGQAAHSGSTPMNRRKDAFLAAAKMSPEIYKIAERHGGVCTIGSCTTKPGIVTSV
                     VEECRITLDQRHLDAAALARMLADAKAAGERFAAEGNVKIAW"
     gene            1074..1343
                     /locus_tag="VG754_11770"
     CDS             1074..1343
                     /locus_tag="VG754_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HWF19941.1"
                     /translation="MTTISLKLPEALARAIAEEAKARSVAKSAVVRECLEQALRRRGK
                     PHVTCLDLMADRIGSFQGPRDLSTNRRYLVKAVNANANRPRKNSR"
     gene            1306..1740
                     /locus_tag="VG754_11775"
     CDS             1306..1740
                     /locus_tag="VG754_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWF19942.1"
                     /translation="MQMPIAHEKILVDTGPLVAVLNAADEHHAWALEVFGRLVPPVFT
                     CEAVLSEAQFLLHDRGGDPLAVLDWVRLGAVSLSFSAGAEIGRLLALQRSYQNLPMDF
                     ADACLVRMTELHSRSRVLTTDAHFRIYRRNGRQQIPVIAPGE"
     gene            1884..>2705
                     /locus_tag="VG754_11780"
     CDS             1884..>2705
                     /locus_tag="VG754_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HWF19943.1"
                     /translation="MPTLATIVDGLKLPNPFVIGSGPPGTNLNVIFKAFQEGWGAVIA
                     KTVSLDASKVINVTPRYAKLLATSGEVIGWENIELISDRPFKLWEDEFKKCKDHYPDR
                     VLIASIMEEYRKDAWIEIVERCQNAGVDAFELNFSCPHGLPERKMGAAMGQNPEVLEE
                     VVGWVMSAAKKPVWAKMTPNVTHIEDPTRAALRGGATGISAINTIRSVMGVNLDTLRP
                     EPTVEGYTTPGGYSCKAVKPIALRMVMEVAQVIRHEFPGRSLSGLGGIENGSDAAQ"
BASE COUNT          577 a          789 c          739 g          600 t
ORIGIN      
        1 tcccacgcga tcttcacatt tccctcggcg gcaaatcttt cgccggccgc tttggcatcc
       61 gcaagcattc gtgcaagcgc ggcagcatca aggtggcgtt gatcgagcgt gatgcggcat
      121 tcctcgacaa cgcttgtgac gatgcccggc ttggttgtgc atgagccgat ggtgcaaacg
      181 ccgccgtggc gctccgcgat tttataaatc tcagggctca tcttcgccgc ggcgaggaaa
      241 gcatccttgc ggcggttcat cggtgtgctg cccgaatgcg ctgcctggcc ctgaaacgtg
      301 atcgcgtgcc gctccactcc gaaagttccc aacacagcgc ccaacggcaa ttcgaggtcc
      361 aacaacaccg gcccttgctc aatgtgcaat tcaagataag cggcggcatt ttgcaattcg
      421 cgtccgctct ccttgacgcg ctcaaaatcc actccaacat ttttcaacgc atcgggcaac
      481 gtgatgccgt ccttgtcctt gagtccgcgc gcctcgtcca gattgagatt gccggaacaa
      541 gctgacgaac cgaacaaact tttgccgaaa cgcgctcctt cttcatctgc ccaatccacc
      601 agtcggaccg tcactggcgg tttgccgtta tattgttcat tgatgcggcg caaaatctcc
      661 acgcccgcca agacattcag gcaaccgtcc agccagccgc cgtttggcac ggaatcaata
      721 tgtccgccga tgagcaatgc gcgttccgat tcaccgcgca acgtagtcca aagattgccc
      781 gcggcatcca tgtgcatttc aaccggcaac gcttcaagtt ttttgcgcaa ccacgcgcgc
      841 gcggccgtcc aaacaggggt gaacgcgatg cgttgcgcgc cgttttcatc accggtgagc
      901 gcgcgaagtt ccttgagttc ggtcacggtg cgtttggggt tcagcaatga actcatatcg
      961 gccaatcctg aggcgacaaa gattattttt caataaaaac cctgcaaaca gggggctgtc
     1021 ttgctttcaa aaatggtatt gcccgttgca acattcggta atacattctc cgcatgacga
     1081 caatttcatt aaaattgccc gaagccttgg cccgcgccat cgcggaagag gcgaaggccc
     1141 gaagcgtggc aaaatccgca gtcgttcggg aatgcctgga acaggctttg cgtcggcgcg
     1201 ggaagcccca tgtcacctgc cttgatttga tggccgacag gatcggttcg tttcaaggac
     1261 cgcgggatct ttcgaccaac cgccgttatc ttgtcaaagc cgtcaatgca aatgccaatc
     1321 gcccacgaaa aaattctcgt tgatacgggg cctttggtcg cggtgttgaa tgcggctgac
     1381 gagcatcacg catgggcttt ggaagtcttt gggcggctcg tccccccggt gttcacttgc
     1441 gaggcggtgt tgtcagaggc gcaattctta ttgcacgacc gcggaggcga tccgcttgcg
     1501 gtgctggatt gggtgcgact cggcgccgtc agcctgtcct tttccgctgg agctgaaatc
     1561 ggtcggttgc tcgcgctgca acgctcgtat caaaatttgc cgatggactt tgcggatgcc
     1621 tgtctcgtga ggatgaccga attgcattcc cgttcgcgtg tccttaccac agatgcccac
     1681 tttcgaattt atcgccggaa cggacggcag cagattcccg tgattgcgcc gggcgaataa
     1741 cggaaaatat attacgtttg cgtaatattt aatttcccac ttttcattgc tgtccatttc
     1801 ccttatcttg gatgtcagta atgtttatca cagcgcgtgc gctgaaaatg cgccgccaaa
     1861 ccgaaaattg aaagtccaaa cctatgccca cccttgccac catcgttgac gggctcaagc
     1921 tgccgaatcc ttttgtgatc ggctccgggc cgccgggcac gaatttgaac gtcatcttca
     1981 aggccttcca ggaaggttgg ggtgcggtca tcgccaagac ggtcagtctc gatgcctcaa
     2041 aggtcatcaa cgtcacgccg cgctatgcca agctgctcgc caccagcggc gaagtcatcg
     2101 gctgggaaaa catcgaactc atcagcgacc gtccgttcaa gctttgggag gacgaattca
     2161 aaaagtgcaa ggaccattat cccgaccgcg tgctcatcgc ttccatcatg gaagaatatc
     2221 gcaaggatgc gtggattgaa atcgtggaac gctgccaaaa cgcgggcgta gatgcgttcg
     2281 aattgaattt ctcctgcccg cacggtttgc ctgaacgcaa gatgggcgcc gcgatggggc
     2341 agaacccgga agtgctcgaa gaagttgtcg gctgggtgat gagcgcggcg aaaaaaccgg
     2401 tctgggcaaa aatgacgccg aacgtcacgc atatcgaaga cccgacccgc gcggcattgc
     2461 gcggcggcgc gaccggcatt agtgccatca acaccatccg cagcgtgatg ggcgtcaatc
     2521 ttgatacctt gcgcccggaa ccgacggttg aaggttacac cacccccggc ggttattcgt
     2581 gcaaggcggt taaaccgatc gctctccgca tggtgatgga agtggcgcag gtcatccgcc
     2641 atgaatttcc cggacgctcg ctttccggcc tcggcgggat tgagaacggc agcgacgccg
     2701 cgcaa
//
LOCUS       DASZMM010000488         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2413106, whole genome shotgun sequence.
ACCESSION   DASZMM010000488 DASZMM010000000
VERSION     DASZMM010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413106"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VG754_11785"
     CDS             complement(<1..598)
                     /locus_tag="VG754_11785"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWF19944.1"
                     /translation="MTTNTRFTQKILTGAVALAIVAFAASARAEAVDQYVTILKVNGH
                     ARYSISGKMNDWKECKKGDVLRPGTLFQTAEDGNVDVIFGERVPVATTPMGAGAPSAR
                     ALSYNPDKGPKANIVHIFPSSVLVVDKLTFEKTGMDDVSETQLDLRAGQIMGNVKKLN
                     GASRYEVKIPNGVAGIRGTTYIIRGDGQVLVFSGSVVVS"
     gene            complement(851..1219)
                     /locus_tag="VG754_11790"
     CDS             complement(851..1219)
                     /locus_tag="VG754_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19945.1"
                     /translation="MKQRHKRIEDAKVPILPVDCNTVITRRTPVAHALKMPLLTELKN
                     VFCLFYKDVTPTAFPMGPSFTHFAFFAANLNPVESAVLIDFVASQSSAVRRGIFVETG
                     MINLQLRQERHIPRIILHLE"
     gene            complement(1194..>2176)
                     /locus_tag="VG754_11795"
     CDS             complement(1194..>2176)
                     /locus_tag="VG754_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF19946.1"
                     /translation="TSIQRRPFKAHNDYLNTLVDWGVVGTALVASAWVLLYWGIIKVW
                     RDVRGRRDDFSRKKSNKFAFLIGASVGLFAILVHSLVDFNMQLPAIAILVVTFMALLS
                     SQWRFATERFWFRMTLPLKSIGTIVLLAGLVYLGLAGVRRGQEYVWLRDAEILEHAPG
                     NYTTARIDVLQKAFAVDPMNFETAYALGESYRSKSWEGGDDYVELAQKAIGWYRRGMK
                     LNPYDPMNWLGCGLCLDWMGSHGEGTENSVTYYNRANALDPNNYFITVETGWHYMQTG
                     DYAAARTWFERSRRLEWKESENRIAFEYAPIAERRLKEDALKNEAKAQAN"
BASE COUNT          519 a          610 c          546 g          501 t
ORIGIN      
        1 aagaaaccac aacggaaccg ctaaaaacca aaacttgacc atcgccgcga atgatgtaag
       61 tcgtgccgcg aatgccagcc acgccgttcg ggatcttgac ttcataccgc gaagcgccat
      121 tgagcttctt cacgttgccc atgatctggc cggcgcgcag gtccaattgc gtttcggaga
      181 catcgtccat tcccgtcttc tcaaaagtga gcttgtccac caccaaaacc gagctgggga
      241 aaatatgcac gatattggcc ttgggaccct tgtcaggatt gtagctcaac gcccgtgcac
      301 taggagcgcc cgcacccatg ggagtcgttg caaccggaac tctttcgccg aatatcacgt
      361 ccacgttgcc atcctccgca gtctggaaca gagtgccggg cctcaacaca tcgcccttct
      421 tgcattcttt ccaatcattc atcttgccgc tgatagaata acgcgcatgg ccattgactt
      481 tgagaatggt gacgtattga tcaacggctt cagcgcgagc gctggccgcg aacgcgacga
      541 tggccagagc aactgcgcca gtgagaatct tctgagtgaa acgagtgttt gttgtcataa
      601 agtcctgact tctcaggttg catttatcgc atgttattta cgcccaatgt ttaccgcttt
      661 gagtccccgc tgtcaatgga ataatgagag gcaaaaccgc ctttatttcg ctgataatca
      721 ggtggattca gcaaggatgg ggccttgtgt gctgttttcg ggtggaaagg caggtgagac
      781 gatcatttct tgcacgtagc gcagatttca atctgctgtt tcaatgattc gaggcggttc
      841 cacgatacat ttattcgaga tgaagaataa ttcgcgggat atgccgctcc tgacggagct
      901 ggagatttat catcccggtt tctacaaaga tgccgcgcct aacggcgctg gattgcgatg
      961 caacaaaatc aatcagaacg gcggattcaa cagggtttag attcgcagcg aaaaacgcaa
     1021 aatgcgtgaa cgacggcccc attggaaacg ccgtaggcgt gacatctttg tagaacaagc
     1081 aaaaaacatt tttaagctcc gtcaggagcg gcatcttcag cgcatgggca acaggagtac
     1141 gtcgcgttat taccgtgttg cagtccacag gtaaaattgg aactttagcg tcctcaattc
     1201 gcttgtgcct ttgcttcatt ctttagcgcg tcttccttga gccggcgctc ggcaatcggg
     1261 gcatattcga aggcgatgcg gttttccgat tctttccatt ccagccggcg cgagcgttca
     1321 aaccaggtgc gcgccgcggc ataatcgccg gtttgcatgt aatgccagcc ggtttcaacg
     1381 gtgatgaaat agttgttggg atcaagcgca ttggcccggt tgtaataagt gacggaattt
     1441 tccgtccctt cgccatgcga gcccatccaa tcaaggcata acccgcaacc aagccagttc
     1501 atcgggtcgt aaggattgag cttcatcccg cgccgatacc atccaattgc tttctgcgcc
     1561 aactcgacgt aatcgtcgcc gccctcccag cttttcgagc ggtaagattc gccaagggcg
     1621 taggccgtct cgaagttcat cgggtcaacg gcaaaggctt tctgcaaaac atcgatgcgc
     1681 gcggtggtgt aattgcccgg cgcgtgttcc aaaatttccg catcgcgcaa ccagacatat
     1741 tcctgcccgc gacgcactcc tgccaatccc aaataaacca aacccgccag caacacgatt
     1801 gtcccgatgc ttttcagcgg cagcgtcatg cgaaaccaaa agcgttcggt cgcaaaacgc
     1861 cattggctgc tcagcaatgc catgaacgtc accaccagga tcgcgattgc cggcagttgc
     1921 atgttgaaat ccaccagcga atgcacgagg atcgcgaaca atcccaccga cgcgccgatg
     1981 agaaaggcaa atttgttgct tttttttcgc gaaaaatcat cccgcctgcc gcgcacgtcc
     2041 cgccacacct tgatgattcc ccaatacaac aacacccatg ccgatgccac caaggcggtg
     2101 ccgacgacgc cccaatccac aagcgtgttc aagtaatcgt tgtgcgcctt gaacgggcgg
     2161 cgctggattg aggtcg
//
LOCUS       DASZMM010000489         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2685098, whole genome shotgun sequence.
ACCESSION   DASZMM010000489 DASZMM010000000
VERSION     DASZMM010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685098"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..2418)
                     /gene="lon"
                     /locus_tag="VG754_11800"
     CDS             complement(16..2418)
                     /gene="lon"
                     /locus_tag="VG754_11800"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWF19947.1"
                     /translation="MTSPDTEFISILGAASGGADAPAERLSARSLPEVLPILGLSDIV
                     IFPGMVAPLLVETSQSIHLIDDVVGGDRLLGVVLQRKPEAENPTPEEMHEIGCAARVL
                     KMLKFPDNTVRVLVEGLWRIRIKGYESQAPYLKAKIEVWKDVKEDSIELAALTRNAQT
                     EFQEIVKLSPAMADQVKIAALNTEDPGHLTDLIAVNLNLSLAERQQMLETNSVKERLT
                     RLLPMLNREREVLTLSSKIQTDVASSMSKTQRDFFLREQMRAIQRELGEGDVTATDVK
                     NLREQIEKSQLPDDARKVALQELERMSQMSPAMAEYGVSRHYLDWILNMPWAKGTEDK
                     IDLTAAKRILDEQHFGLQKVKDRLLEFLAVIKLKKQIKGPILCLVGPPGVGKTSLGKS
                     VAEALGRKFARISLGGMRDEAEIRGHRRTYVGALPGRILHALRRTESNNPVILLDEID
                     KVGADFRGDPSSALLEVLDPAQNSTFTDHYLDLPFDLSRVLFVTTANWLDPIHPALRD
                     RLEVIELPGYTESEKLQIAKRYLVPRQLDENGLTRQIFKIPDAALRKLIQDYTREAGV
                     RQLEREIAALMRKAALKIVNQNGESRPLALTPESLAEYLGPPRNFSETAETITELGIA
                     TGLAWTPVGGEILFIEATRMPGKGQLILTGSLGEVMKESAQTALSYLRSQATAMGVDL
                     SDYNKYDIHIHVPAGATPKDGPSAGVTIVVALASLLLRRRVRSDTAMTGEISLRGRVM
                     RVGGIKEKVLAAARFGIKHVILPEQNRSDWSEVPAEVRKKMNAHFVKHISELVPLALR
                     PK"
     gene            complement(2476..2880)
                     /locus_tag="VG754_11805"
     CDS             complement(2476..2880)
                     /locus_tag="VG754_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWF19948.1"
                     /translation="MCKVSTSVHFVQRPSVVGERQAHWVPNTDVYVTDDGLVIKVELA
                     GMRSENLQITVEGNKLRISGQRPDGCRAAKFSFLAMEINYGPFENILELPAGYDLSLA
                     KASYLNGFLRIDVPQAVKSPSSKRTKVPIAEK"
     gene            3419..>3783
                     /locus_tag="VG754_11810"
     CDS             3419..>3783
                     /locus_tag="VG754_11810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWF19949.1"
                     /translation="MCSKLLRLVLADTAAVHQNLFGAWMPDAGALPFVMPVFQYVHRV
                     TYSECTAGNHVYYARYLDLLEIARGEFFRRLGAPFLQWQQQEIIFPVIECRVRYKSPA
                     RYDDLLTVELWPTAVERIRL"
BASE COUNT          802 a         1122 c         1000 g          859 t
ORIGIN      
        1 atcggctcaa cccgcttatt tgggacgcaa cgccagcggc accagttcgg aaatatgctt
       61 cacgaaatgc gcattcattt tcttgcgcac ttcagccggc acttcgctcc aatccgaacg
      121 gttctgttcg ggaaggatga cgtgcttgat gccgaaacgc gccgcggcca gcactttttc
      181 cttgatgccg cccacgcgca tgacgcgtcc gcgcaaactg atttcgcccg tcatcgccgt
      241 gtccgaccgc acgcgccgcc gcagcaacaa cgacgccagc gcaaccacaa tcgtcacacc
      301 ggcgctcggc ccatccttgg gcgtcgcccc ggccggcacg tggatatgaa tgtcgtattt
      361 gttgtaatcg ctcaaatcca cgcccatcgc cgtggcctgg ctgcgcagat aactcaatgc
      421 cgtttgggcg ctttccttca ttacttcgcc gagcgagccg gtcaaaatca actgtccctt
      481 gcccggcatt cgcgtcgctt cgatgaataa aatctcgccg ccaacaggcg tccatgcgag
      541 gccggtggcg attccgagtt cagtgatggt ctcggcggtc tcggaaaaat tacgcggtgg
      601 cccaaggtat tcggcgagcg attccggcgt cagcgccaat ggccgcgatt cgccgttctg
      661 gttgacgatt ttcaaggccg ccttgcgcat caacgcggcg atttcgcgtt ccaactgccg
      721 cacgcctgct tcgcgcgtgt aatcctggat aagcttgcgc aatgcagcgt cgggaatttt
      781 gaaaatttga cgcgtgaggc cgttttcatc cagttgacgc ggcacgagat aacgcttggc
      841 gatttgcagt ttttcggact ccgtgtaacc gggcagttcg atcacttcca aacggtcgcg
      901 caatgcggga tggatcgggt cgagccagtt cgcggtggtg acgaacaaca cgcgcgacaa
      961 atcaaacggc aaatcaagat aatgatcggt gaacgtgctg ttctgcgccg ggtccagcac
     1021 ttcaagcagt gcggaggacg ggtcgccgcg gaagtccgcg ccaactttgt cgatttcatc
     1081 cagcaaaatc acgggattat tgctctctgt gcggcgcaac gcgtgcaaga tgcgtcccgg
     1141 caacgcaccc acataagtgc ggcgatgtcc acggatttcc gcttcgtcgc gcattccgcc
     1201 caatgaaatc cgcgcgaatt ttcggcccaa cgcttccgca acacttttgc ccaaagacgt
     1261 cttgcccacg ccgggcgggc cgacaaggca aagaatcggc cctttgatct gtttcttgag
     1321 cttgatgacc gcgagaaatt ccagcaaacg gtccttcacc ttttgcaatc caaaatgttg
     1381 ctcgtccaaa atgcgcttgg ccgcggtcag gtcgatcttg tcctcagtcc ccttggccca
     1441 cggcatgttc aaaatccaat ccagataatg ccgcgacacg ccgtattccg ccatcgcggg
     1501 cgacatttgc gacatcctct ccaattcctg aagcgcgacc ttgcgggcat cgtcaggaag
     1561 ctgagatttc tcaatctgtt cgcgcaaatt tttgacatcc gtcgcggtga catcgccttc
     1621 gccaagttcg cgctggatgg cgcgcatctg ttcgcggagg aaaaaatcgc gttgcgtctt
     1681 ggacatcgaa ctggcgacgt ccgtctggat ttttgagctg agcgtgagca cttcgcgctc
     1741 gcgattgagc atcggcagga gccgcgtgag acgttccttg acggaattgg tttcaagcat
     1801 ctgctggcgc tcggcaaggc tcaagttcaa gttgaccgcg atcaaatcgg tgagatggcc
     1861 cgggtcttcc gtgttcaacg cggcgatctt gacctggtcg gccatggcgg gcgaaagctt
     1921 cacgatttcc tggaactcgg tttgcgcatt gcgcgtcaac gcggcgagtt caattgaatc
     1981 ttcctttacg tctttccaga cttcgatctt ggccttcaga tacggcgcct ggctttcgta
     2041 acccttgatg cggatgcgcc ataatccctc gaccagcacg cgcaccgtgt tatcgggaaa
     2101 cttcaacatc ttcagcacgc gcgccgcgca accgatttcg tgcatctctt ccggcgtggg
     2161 attttccgcc tccggtttgc gctgcaacac cacgcccaac aagcggtcgc cgccgacaac
     2221 gtcgtcgatg agatggatgc tttgcgaagt ttcgacgagc aacggggcga ccatgcccgg
     2281 gaaaatcacg atgtcggaaa ggccgaggat gggcagcacc tccggcaacg aacgcgcaga
     2341 aaggcgttct gccggagcgt cagcccctcc ggatgcagct cctagtatgc tgatgaattc
     2401 tgtgtcgggc gatgtcatgg tcgcgccatt tttgaattag aaagccgccg aacccaaaag
     2461 aacccagaac cactcctact tctccgcgat cggcaccttg gtgcgtttcg aagaagggga
     2521 cttgacggct tgcggcacgt caattctgag aaagccgttg agataagaag cctttgccag
     2581 gctgagatca tatccggcgg gcaactccaa aatattttca aacgggccgt aattgatctc
     2641 catggccaaa aagctgaatt tggcggcgcg gcaaccgtcg ggccgctgtc cgctgatgcg
     2701 aagtttgttt ccctcaacgg tgatctggag gttttcgctt cgcatcccgg ccagttccac
     2761 cttgatcacc aatccatcgt ccgtgacata gacgtctgtg ttgggcaccc aatgggcctg
     2821 tcgttcacca acgacggaag gtcgttgcac aaaatggacg gacgtactga ctttgcacat
     2881 ggccatgtag ggttaaagaa tagtcaaggt gttacagtaa tgcaagattc gttatttcat
     2941 tttgaccgtg atttgaggcc gttcgccgcg ccgcgcccgg aaaaccatcg tgctttgagg
     3001 agcggtatgc cccacggacg agcacccgca atgtgtatct cgttccaacc ggaacttccg
     3061 cggacgcaat ctaatccaag cgaaagccgc aatggtgaca cccacgattc ccaatgaaac
     3121 aatatgctgc caatccacga ttccaagata ctacctttcc cccacggtgc aacaaataac
     3181 atcgaacgct gaacactgaa cgtcgaacat cgaatgacaa ccgaccgcaa tttcattggg
     3241 cattcgacgt ttctcattcc gtcacacctt ggtccgtggt ttcccattct ccactagctc
     3301 aggggggtca ggcgccgtca ctacactagg aatagagatt atttgaagtt acaacacatc
     3361 tcaaaatgtg gaacacggct gtgtgcgaag cgtcaaccgc gcacactgcc aaaatcccgt
     3421 gtgttccaag ctgctgcggc tggtccttgc ggacacagcc gcggtccatc aaaacttgtt
     3481 tggagcttgg atgccggatg ccggggcttt accatttgtc atgccggttt tccaatatgt
     3541 ccaccgcgtc acttattccg aatgcaccgc ggggaaccac gtttattacg cgcgttatct
     3601 tgacctgctt gaaattgcgc gaggcgaatt tttccggcgt ctcggcgcgc ctttcctcca
     3661 atggcagcag caggaaatca tctttcccgt catcgaatgc cgagtccgtt acaaaagtcc
     3721 cgcgcgctat gatgatctcc tcaccgttga actctggcca accgccgtcg agcgcattcg
     3781 gct
//
LOCUS       DASZMM010000490         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3513192, whole genome shotgun sequence.
ACCESSION   DASZMM010000490 DASZMM010000000
VERSION     DASZMM010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3513192"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..504
                     /locus_tag="VG754_11815"
     CDS             304..504
                     /locus_tag="VG754_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19950.1"
                     /translation="MSSVKEIEAAISNLSREELSSFRTWFADFDAEVWDKQFEQDVKA
                     GRLDALAEEALRDLDEGRCTDL"
     gene            501..764
                     /locus_tag="VG754_11820"
                     /pseudo
     CDS             501..764
                     /locus_tag="VG754_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019493160.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            849..2189
                     /gene="ffh"
                     /locus_tag="VG754_11825"
     CDS             849..2189
                     /gene="ffh"
                     /locus_tag="VG754_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWF19951.1"
                     /translation="MFDALSSKLQNAFKNLRGLGKISETNVADSLREVRLALLEADVN
                     FKVARDFIERVKTKALGQEVIQSVQPGQQIIKIIHDELVELLGSENAGLNLSGNPACV
                     LMVGLHGSGKTTSSAKLARMLLKQGRTPLLVAADVYRPAAMDQLETLAKQIEIPVFVQ
                     KGETDVLKIARGALEFAKANNRNTLIFDTAGRLQIDEPLVQELVRLRDLVKPQEVLLV
                     LDAATGQEAVNVATHFDQALHITGSILTKLDGDARGGAALSMKSVTGKPIKLAGVGEK
                     VDEFEPFHPERMASRILGMGDVVSLVEKAAEAYDADEAKRMEEKMRKGQFTLEDFLEQ
                     LRQMKKLGPLENIVGMLPGGSEMMKGADMSKQEKEFAHMEAMICSMTLQERKQPQILN
                     AKRRIRIAKGSGVTVTELNTMLNKFGQMQQMMKKMGRFQKMMMKRGGMPGMFGR"
     gene            complement(2205..2780)
                     /locus_tag="VG754_11830"
     CDS             complement(2205..2780)
                     /locus_tag="VG754_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HWF19952.1"
                     /translation="MNAKTAFRLACFLFVSNFSIIARADDFQLEPSYISLFNGHDLTG
                     WGFRTNNFDGKTESNDGRYSAKDGILIVHPHTPRVVEVIWTTRQFPKNFNLKLEFRAS
                     YNADSGLFVRDPQLQVRDYLVAGPYKQLKKYKPQDWNVIDVTVKDGAAFCTCNGEVLE
                     EAMKVPANGPIGLEADRGQVEYRNIRLKELP"
     gene            2957..3205
                     /locus_tag="VG754_11835"
     CDS             2957..3205
                     /locus_tag="VG754_11835"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HWF19953.1"
                     /translation="MKISLTPHQEKFIASKMRNGGYLSRSEVVREAMRVYEMTELQDN
                     DPELEAALRHSLSSPRKKYTPGHFAALASQNRRRSAAA"
     gene            3202..>3468
                     /locus_tag="VG754_11840"
     CDS             3202..>3468
                     /locus_tag="VG754_11840"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF19954.1"
                     /translation="MNRVEIIVRHDAELDAFNYFQYLHERNSDAALHFLEAVDQTVED
                     LALHPLKGRKRHFRGRDLRDIRSWRVSGFENYLIFYRFRGTCLEI"
BASE COUNT          831 a          867 c          985 g          785 t
ORIGIN      
        1 gtgtaggtgt tggccacgca ccgaagatgc cgctcctgac agagcttgaa aatacttttt
       61 cccttgttct agaaagatgt cacgcctacg gcgttctaat gggccagcgt ttggcgcttc
      121 ttggattttt cgccggcagt caaaaccagg ttgaatccgc tgcaccactg ccgtagttta
      181 catttatgtt cgatgccctg agcagcaaac tccagaacgc gttcaagaac ctgcgcggct
      241 caactttggc tggtcaggcc acttggccct tgccagttcg gatggttgtc gtatttttgt
      301 tgcatgagca gcgttaaaga gattgaagca gccattagca atctttcgcg cgaggagctt
      361 tcatcctttc gcacatggtt tgcggatttt gacgcggagg tttgggacaa acaatttgag
      421 caagatgtca aagccggccg tctggatgct ttggcggagg aggcattgcg ggacttggac
      481 gagggacgtt gcactgacct gtgagacatc gcgccagcaa gaagttttgg cagcattgtg
      541 aacagcttcc agttgatatt cagaacctgg cagaccgctg tttctagatt ttgaagcgag
      601 accttcatca tccttcacta cacttcaaga aagttgggcg attttggtcg gtgcgtgtcg
      661 gcatccatta tcgagcagtt cccgttgaac gggattcaga catggtatgg ttttggatag
      721 ggcgccatga ccggtatgac tttgtaattg cagaccgtca ataaagaaca gcagcctcac
      781 ccgaggcggg aggcgcacgt ttacgaaatg cggttgaatc cctccgacca ctgccgtagc
      841 ttacgcttat gttcgatgcc ctgagcagca aactccaaaa cgcgttcaag aacctgcgcg
      901 gtctcggaaa gatttccgaa accaacgtcg ctgattcgtt gcgcgaagtg cggctcgcgc
      961 ttttggaagc ggacgtgaat ttcaaggtcg cccgcgattt cattgagcgc gtcaagacca
     1021 aggcgctcgg gcaggaagtc atccagagcg tccaacccgg ccagcaaatc atcaagatca
     1081 tccacgacga actggtcgaa ctgctcggct ctgaaaatgc gggcctgaat ttgagcggca
     1141 atcccgcttg cgtgttgatg gtcggcttgc acggttccgg caaaacgaca tcgagcgcaa
     1201 agcttgcgcg aatgctgttg aaacagggcc gcacgccgtt gctcgtcgcc gcggacgttt
     1261 atcgccccgc cgccatggac cagttggaga cgctggcgaa acagattgag attcctgtct
     1321 tcgtgcaaaa gggcgaaacg gacgttttga agattgcgcg cggggcattg gaatttgcga
     1381 aggcgaacaa tcgcaatact ttgattttcg ataccgccgg gcgattgcag attgacgaac
     1441 cgctggtgca ggaattggtg cggttgcgcg acctcgtcaa gccgcaggaa gttttgctcg
     1501 tcctcgatgc cgccaccggc caggaagcgg tcaatgtggc aacgcatttt gatcaggcgc
     1561 tgcacatcac gggttcgatt ttgaccaagt tggacggcga cgcgcgcgga ggcgcggcat
     1621 tgagcatgaa gtccgtcacc ggcaagccga tcaagctggc aggcgtcggc gaaaaggtag
     1681 atgaattcga gccattccat ccggagcgga tggcgtcacg cattttagga atgggcgatg
     1741 tggtgagcct ggttgaaaag gccgccgaag cgtacgacgc ggacgaagcc aagcgcatgg
     1801 aagaaaagat gcgcaagggc cagttcacgc tggaagattt tcttgagcag ttgcgccaga
     1861 tgaaaaagct cgggccgctc gaaaacatcg ttggcatgtt gccgggcggt tcggagatga
     1921 tgaagggcgc ggacatgtcc aaacaggaaa aggaattcgc gcacatggag gcgatgattt
     1981 gctcgatgac gctgcaggaa cgcaagcagc cgcaaatcct caatgccaag cgccgcatcc
     2041 gcatcgccaa gggcagcggc gtgaccgtca ccgaactcaa caccatgctc aacaagttcg
     2101 gtcagatgca gcagatgatg aagaagatgg gccgcttcca gaagatgatg atgaaacgcg
     2161 gcggaatgcc cgggatgttc ggacgctaat cgggaattcc tgagttacgg caattctttc
     2221 aaccggatgt tccggtattc cacctggccg cgatcggctt cgaggccgat ggggccattc
     2281 gcaggcactt tcatcgcttc ttcgagcact tcgccgttgc aggtgcaaaa tgccgcgcca
     2341 tccttcaccg tcacgtcaat cacattccaa tcctgcggct tgtatttctt aagctgcttg
     2401 taggggcctg cgaccaggta atcgcgcacc tgaagttgcg gatcgcgaac gaacagtccg
     2461 ctgtcggcat tgtaactggc gcgaaattcg agcttgagat tgaagttctt tggaaattgt
     2521 cgcgtggtcc agatcacttc aaccactcgc ggcgtgtgag gatgcacaat caaaatgcca
     2581 tccttcgctg agtaacggcc gtcgttggat tccgttttgc cgtcgaaatt gtttgtgcga
     2641 aaaccccagc cggtcaggtc gtggccgttg aagagactga tgtaactggg ttcgagttgg
     2701 aagtcgtcag cgcgtgcgat gattgaaaag tttgaaacaa acaggaagca agcgagtcga
     2761 aatgcagttt tcgcattcat aattattagg tgtggctgaa atctgcttgc aatcccgtcc
     2821 cgcggcaagc tgaaaaggat agctgaaaaa gaaggcattt cgcattcaac gcgatgctcg
     2881 cgaagaacat ggtcgcgaca ggacggaact ttgcatctat tttgacacaa agcctgcatt
     2941 catttaatct gaagtcatga aaatttcgct cacgccgcac caggaaaaat tcattgcgag
     3001 taaaatgcgg aatggcggct atctttcgcg gagtgaagtc gtgcgcgagg caatgcgcgt
     3061 ctatgaaatg accgaactgc aagacaatga tccggaactg gaggcggcat tgcgccattc
     3121 gttgagcagt cccagaaaaa aatacacacc cggccatttt gccgcgctcg cctcacagaa
     3181 caggcgccgt tctgccgccg cgtgaacaga gttgaaataa ttgtccgcca tgacgcggaa
     3241 ttggatgcgt tcaattattt ccaatacctt catgagcgaa attccgatgc cgccctccac
     3301 tttctcgaag ccgtagacca aactgtcgaa gacttggcgt tgcacccatt aaaaggaaga
     3361 aagcggcatt ttcgtggaag agatttgaga gacatccgtt catggcgggt aagcggattc
     3421 gagaattatt tgatctttta ccgctttcgt ggaacctgcc tggaaatt
//
LOCUS       DASZMM010000491         4594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_205029, whole genome shotgun sequence.
ACCESSION   DASZMM010000491 DASZMM010000000
VERSION     DASZMM010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205029"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VG754_11845"
     CDS             <1..927
                     /locus_tag="VG754_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HWF19955.1"
                     /translation="RKVYDLYGPSEDTTYSTWMLRRADGPETIGRPMTNKQVYLLDAL
                     LQPVPVGVTGELYIGGAGVARGYLHRPELTAEKFIPDPFSQKSGARLYKTGDLARYLP
                     DGNIQFLGRIDHQVKVRGFRIELGEIESVLRRHPDIRETIVVARADQNGEKRLIAYVV
                     ADQQPTLTPRELKTHVASLLPEYMVPSAFVLMDALPLTPNGKVDRKALPEPESQSENC
                     DSIPPSTPAEMAVAKLWCEVLGVKQVGIHDNFFELGGHSLMATQLISRFSKFHKIQVK
                     LRDIFNAPTVAEMSRWLERKEMGKSVSEPATA"
     gene            complement(1131..3392)
                     /locus_tag="VG754_11850"
     CDS             complement(1131..3392)
                     /locus_tag="VG754_11850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWF19956.1"
                     /translation="MTIEEKKLESSNCSDCGAELSHSTGHCLACLLQLGLDAGMEEIP
                     LLNSGDWVGRYELLEQIGEGGCGVVFMAEQSQPVRRRVALKVIKPGMDTRQVIARFEA
                     ERQALAMLDHPNIAKIFDAGATESGHPFFVMELVRGVKITDYCDQQKLSVRERIELLA
                     QICHAVQYAHQKGIIHRDLKPSNILVGEDGRPTIIDFGIAKSMERPLTEKTLFTAFQQ
                     FIGTPAYMSPEQAGTGDVDTRADIYSLGILLYELLTGLTPFDAEELRRLAPDEILRFI
                     RNAEPLRPSARLTRLTHSQLSNIAQCRRIEPTKFLHLLRGDLDRIVMKCLEKEPASRY
                     ETASSLALDLQRYLAHEPVSARPPGHWYRFEKMVRRNRVVAGAAAAIALALILGTIVS
                     AYQAVRAGRAEQEQAQLRREAQAEAAKNAEVAQFLKAILQGAGPSVALGRDTTMLREI
                     LDRAAARLSRDLTNQPEAQAELFSTLADTYHQLGFYEKMEAMARESLRLSRSKSGVEN
                     IAVIRALAQIGDADEHLGKLDEAERFTRQALEMRRKLPGDAEPELSGLINNLGLVLRE
                     QGRLREAEILFRDALAMDQKKSGGSGEKAATDLSNLGAVLLSEGQLEEAESKYRDTLA
                     LRRKIFGDEHPEVAIALGNLSVVLARERKFAESEATARETLALDRKFLSADHPDLPLA
                     LANLASVFAAQGKWPEAEAADREALADYQKLSGTNHTEESATLENLAKDLSQQGRLAE
                     AETLKNKMQAIGK"
     gene            complement(3409..4296)
                     /locus_tag="VG754_11855"
     CDS             complement(3409..4296)
                     /locus_tag="VG754_11855"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF19957.1"
                     /translation="MNLVNQPSPRLVMPAFCSKSDRRLSTSLTPCRKVREVAGTMAKS
                     AHHNSPDGKPYAGTGRTGAFSTTHWSVVLTAGSGDPAHSAAALEKLCRQYWHPIYAFV
                     RRRGADRHEAEDLTQSFFAHLLDKESLKKVDRQKGKFRTFLLASLTNFLANEWDKRRT
                     LKRGGEYQIMPLHEVAEEMYKCEPAGSATPEKLFDRCWALLLVEAVRTRLKAEYLAQK
                     KVELFAKLESGLTGELSAGWYGNIAAELGMSEGATRVALHRLRRRFGDLLREEVAQTV
                     GSNAEVDEEIRHLFAVMTR"
BASE COUNT          963 a         1361 c         1238 g         1032 t
ORIGIN      
        1 cgcaaagtct atgacctgta tgggccgtcg gaggacacga cctattccac atggatgctc
       61 cggcgcgccg acggtccgga aaccatcggc cggccgatga cgaacaagca ggtttatttg
      121 ctggatgcgc tgttgcagcc cgtgccggtg ggagtgacag gagaacttta tatcggcggc
      181 gcgggcgtcg cacgaggata cctgcatcgg cccgaactca ctgcggaaaa gttcattccc
      241 gatccgttca gtcaaaaatc cggagcgcga ctttacaaaa ctggcgacct cgcgcgttat
      301 ctgccggacg gcaacatcca atttcttgga cgcattgatc accaggtgaa ggtgcgtggg
      361 tttcggattg aattgggtga aatcgaatcc gtcttgcgcc ggcatccaga cattcgcgag
      421 acaattgtcg ttgcgcgcgc cgatcagaac ggcgaaaaac gtctcatcgc ttacgtcgtc
      481 gctgaccagc aaccgacgtt gacaccgcgc gaactgaaaa cgcacgtcgc ctcgctgctt
      541 cccgaataca tggtcccttc cgcatttgtg ctgatggacg cgctgccgct cacgcccaac
      601 ggcaaggtgg accgcaaagc cctgccggaa cccgaaagcc agtctgaaaa ctgcgactcc
      661 attccgccga gcacgccggc agaaatggct gtggcgaagc tctggtgcga agtcctgggc
      721 gtgaagcaag ttggcattca cgacaatttt ttcgagcttg gcggacattc gctgatggcg
      781 acgcaactca tctcgcgttt cagcaagttc cataagattc aggtgaagct gcgcgatatt
      841 ttcaatgcgc ccaccgttgc cgaaatgagc agatggctcg aacggaagga aatgggaaaa
      901 agcgtttccg aacccgcaac ggcttgagcg cgggttggac ctggcgctgc ccgaaggctc
      961 aacgccaagt ccagtcccat cgtgtcggaa ggttcccggc ccttcctcaa tccacctccg
     1021 aaatcaaaga ttctcttaca ctgcttcata agcttcggtg gggaattgtt acaagaattc
     1081 ggacgaaatt ttttggaggg ccaatccgct caattttgaa tcccttcatt tcatttgccg
     1141 atcgcctgca ttttattctt caatgtttcg gcctccgcca gtctgccttg ctgagaaagg
     1201 tctttggcca ggttctccaa tgtcgcgctt tcttccgtat gattggttcc ggaaagtttt
     1261 tgataatcgg cgagcgcttc gcggtcggct gcttcggcct cgggccattt gccttgcgcg
     1321 gcaaacacgc tggccagatt ggccaatgcc aacggcaaat caggatggtc tgcgctcaaa
     1381 aattttcgat ccaacgccaa tgtctcgcgg gctgtcgctt cggattccgc aaatttccgt
     1441 tcgcgggcca gcaccaccga gagattgccc agcgcgattg cgacttcggg atgttcatcc
     1501 ccaaaaattt tccggcgcaa cgcaagcgtg tcgcgatact tgctttccgc ttcttccaat
     1561 tgcccttcgc tcaacaacac cgcgccgaga tttgaaaggt cggtcgccgc cttttcaccg
     1621 ctgccgcccg attttttttg gtccatggcg agagcgtccc gaaagagaat ctccgcttcg
     1681 cgcaatcttc cctgctcccg cagcaccaat cccagattat taatcaaccc gctcaattcc
     1741 ggctcggcgt cgcccggcaa tttgcgccgc atctccaagg cttggcgcgt gaaacgctcg
     1801 gcttcatcca attttccaag atgctcatca gcatcgccga tttgcgccaa cgccctgata
     1861 acggcgatgt tctcaacgcc cgacttggaa cggctgagtc gcaacgattc gcgagccatg
     1921 gcctccattt tttcgtaaaa gccaagttga tggtaagtgt cggcaagcgt gctgaacaat
     1981 tccgcctgcg cttcgggttg gttggtcaaa tcgcggctta atcgcgccgc ggcacggtcc
     2041 agaatttcgc gcaacatcgt cgtgtcccgt cccaacgcca ccgacggccc cgcgccttgc
     2101 aaaatcgctt tgagaaattg cgcgacctcg gcgtttttcg ccgcttcggc ttgcgcctcg
     2161 cgccgcaatt gcgcttgttc ctgttccgcg cgtcccgccc gcaccgcttg atacgcgctg
     2221 acgattgttc ccaaaatgag cgcaagcgcg attgccgccg ccgcgcctgc aaccacgcga
     2281 ttgcgccgca ccattttttc gaaccgatac caatgtcccg gcggacgcgc gcttacaggt
     2341 tcgtgcgcaa gatagcgctg caaatcgagc gccagactgg acgcagtttc gtaacgagat
     2401 gcaggttctt tttcgaggca cttcatcacg atccgatcca agtcgcctcg caacagatgt
     2461 aaaaattttg tcggttcgat ccggcggcat tgtgcaatgt tgctaagttg cgagtgagtc
     2521 agccttgtca aacgcgcgga cggacgtagc ggttcggcgt tgcgaatgaa gcgcaggatt
     2581 tcgtcaggcg cgagacggcg gagttcttcg gcgtcaaaag gcgtcaagcc ggtcagcagt
     2641 tcatagagca agatgccgag cgaataaata tcggcgcgcg tgtccacgtc gcccgtgccc
     2701 gcctgttcgg ggctcatgta ggccggcgtg ccgatgaatt gctggaacgc ggtgaagagc
     2761 gttttttcgg tgagcggccg ttccatggat ttcgcgatgc cgaaatcaat gatcgtcggc
     2821 ctaccgtctt cgccaacaag aatgttcgac ggtttcaaat cgcggtgaat gattcccttt
     2881 tggtgcgcgt attgaaccgc gtggcaaatc tgagcaagca attcaatccg ctcgcgcacg
     2941 gagagttttt gttggtcgca ataatcggtg atcttcacac cccgcaccag ttccatcacg
     3001 aaaaacggat gtccggattc cgtcgcgccc gcgtcgaaaa tcttggcgat gttcggatgg
     3061 tcgagcatcg cgagcgcctg ccgttcggct tcgaaccgtg caatgacctg acgcgtgtcc
     3121 atgccgggct tgatgacctt gagcgccacg cggcggcgaa caggctggga ttgttccgcc
     3181 atgaacacca cgccgcaacc gccttcgccg atttgttcga gcaattcata tcgcccaacc
     3241 caatcgccgg agttcaacag tggaatttct tccatccctg catccagacc gagttgcaaa
     3301 aggcaggcaa ggcaatggcc ggtgctgtga gataactcag cgccgcaatc tgagcagttt
     3361 gaagattcga gctttttctc ttcgattgtc atggcagcag tcatcttttc atctcgtcat
     3421 caccgcgaac aaatgcctta tctcctcgtc aacttccgcg ttgctgccaa cggtttgggc
     3481 gacttcctcg cgcaacaaat ccccaaacct gcgccgcaac cgatgcaacg ccacgcgcgt
     3541 agctccttcg ctcatgccca gttccgccgc gatattccca taccaacccg cgcttaattc
     3601 acccgtcaat ccagactcca gcttcgcgaa cagttcaacc ttcttctgcg ccaggtactc
     3661 cgccttgagc cgcgtccgga cagcttccac cagcagcaat gcccagcacc ggtcaaacaa
     3721 tttttccggc gtcgccgagc cggctggttc gcatttatac atttcctcgg cgacttcgtg
     3781 cagcggcatg atttgatatt cgccgccgcg cttgagagtg cggcgcttgt cccattcgtt
     3841 ggcgaggaaa ttggtgaggg acgccagcaa aaatgtgcgg aacttgccct tttgccggtc
     3901 cactttcttg agcgattcct tgtcgagcag gtgcgcgaag aaagactggg tcaaatcctc
     3961 cgcttcatgc cggtccgcgc cgcgccgccg aacgaacgca tagatgggat gccaatattg
     4021 ccggcacaat ttctccaacg ccgccgccga atgcgccggg tcgccgctgc cagccgtcaa
     4081 caccacgctc caatgcgtgg ttgaaaacgc gcccgtcctg ccagtcccag cgtacggttt
     4141 cccgtccggc gaattgtgat gcgcggactt ggccatcgtt cctgccactt cacggacttt
     4201 gcggcaaggt gtcaagctgg ttgagagccg acggtcggat ttcgagcaaa atgccggcat
     4261 gacaaggcgc ggcgaaggct ggttaacgag gttcattcca gaaaaaaata tttttttatc
     4321 aggaattaag cggaacgggg tggaaacaag cggaattaag cggaaatttc tccgcgatgg
     4381 tggcggaaaa ttcgttgatg aaacgcaaag gcgcagagac acgagggcgc aaagaaaaga
     4441 agacagttga accccccgat aagggtcgtt cggtgtccat gctttagcat gttccctgcg
     4501 gttgtgccga tgcacgctaa agcgtggaca ccgaacaatc ggattagctg aaacgctcgg
     4561 cgcttcgaag gctgtttggt gtccatgctt tagc
//
LOCUS       DASZMM010000492         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4611479, whole genome shotgun sequence.
ACCESSION   DASZMM010000492 DASZMM010000000
VERSION     DASZMM010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4611479"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..1045
                     /locus_tag="VG754_11860"
     CDS             473..1045
                     /locus_tag="VG754_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWF19958.1"
                     /translation="MNRWITDFIAAQKAALDSIPAKAVAQVIETFQKALAEDRQIFVF
                     GNGGSAANASHFVTDLGKGSSDKIGRRFRVSALNDNVSWMTAIGNDYAYDEVFVRQLM
                     NYGRPGDIVLALSVSGNSPNVVKALEWSVANGLHVIALVGGKRGRMAEIAHQSIVIDS
                     THYGRVEDAQMGICHMICYAFMENPEIGKV"
     gene            1289..3352
                     /locus_tag="VG754_11865"
     CDS             1289..3352
                     /locus_tag="VG754_11865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWF19959.1"
                     /translation="MNFFQQQEQARRRSKRLIILFFLSVLVMMVVVYAIVTPCVLAFR
                     EFRHEHLGVFPSLIMMWFGIPMAVMEAITEPAKFLKDAWRPWMFTGISVGTLLVVASG
                     SIFKMIQLSRGGSVVAELLGGRLLGQRPSDVDELKLRHVVEEMAIASSMPMPQIYVLD
                     NERGINSFAAGHSTSDVAIGVTRGCLKLLTRDELQGVVAHEFSHILNGDTRLNMRLMG
                     LAHGMLFPSILGRMLVHEWTAEDGLATSIFDKRPFAATPFLVVGFALQLTGWIGLPFV
                     RVLKSAICREREWLADASAVQFTRNPEGIAGALKKIGGLIMRGRLRTPHAETASHLYF
                     VNCSTDAFFQIFATHPPLEKRISAIYPYFDGQFPHVNMLPMSEGERDTRLEESIATLV
                     LSEPAQKMVVSTGEKMITPEQAREISQSFRSAALLRQAIPSDVSQAAHQPAGAMAFIY
                     ALLLDADAEARALQLKELQANAPPDIYKQAIGLAAQTGTLDERAKLALIDMALPALRA
                     LTFDQYQEMMRNTEALIESDHGIVLFEYTMQKVLERHLSPRFHKMPAPRVDYRSLQPL
                     AGDCAVLLSALAHMDQDDPAVARNAFTAGVEQLDLRDCQISLLDSKNCNLPQIDVALQ
                     RTGRGSLYVRRNLMYACSHTAAANGRVAGREILLLRAIADALDCPVPPFVDAAEMRAQ
                     SQVNA"
     gene            complement(3383..4267)
                     /locus_tag="VG754_11870"
     CDS             complement(3383..4267)
                     /locus_tag="VG754_11870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF19960.1"
                     /translation="MSERIGFVGVGRMGSNMARRLKEQGYTVSAVYDVNAPAAQALAQ
                     ELGCEAATELKQVTKLSDIIITVVTDDAAMKKIYSGGLLSRAKGKLFINCATISPDMH
                     VKVEEKAAKSGAQALEACMASSITQARQGTLYLMCGGKPEAFERAKPVLEKLSASLRY
                     IGPAGAAAKVKALVNMVMNINTAGLAEGLGLGAALGLDLTMLREVFSQTGANSRVLAT
                     DGEDMQNRDHSCFFSAAHAAKDSGIALKLGKSQGLNLPLATATKKQYDKMVAEGLGEL
                     DKSGIAELTFKGRHETKP"
     gene            complement(4170..>4383)
                     /locus_tag="VG754_11875"
     CDS             complement(4170..>4383)
                     /locus_tag="VG754_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19961.1"
                     /translation="KVVPFRSNSFISSRDFQCAEAPLLAAGCNNLKPQPGLNYVGKNW
                     IRRSRPNGQQHGAPFEGTGLHRLGGL"
BASE COUNT          880 a         1211 c         1294 g          998 t
ORIGIN      
        1 gtaattggcg ataaacgcgc caagggtgat gttggtgttc agatcacatg ccccgccgca
       61 tgccgacaca taaatcaccg gacggatggt caggccggcc gcattgccgt cggcgacgat
      121 gtccgaggac cactcgttga atagtggagc gcggctgtgc acgaagtctc agccgcagca
      181 aactatcgaa acctgagtct tccaagctgc tgcgactggt cctgcggaca cagccgcgct
      241 ccgttgggcg catccgcgca ctgcccccgg agcgcgggtc ggcgacccgc agcaatgtgg
      301 gaaggatggg cgcgcatgaa ttagtccggc acgtcctgtt cgtcgcgcgt tgctgcggct
      361 catcgagccg cgctccgttc tccatttatg agatgatctg taattaaatc tttcgcggaa
      421 ccgcattctt tcctctgtgc attttttggc tatctggcat aattggccga ccatgaaccg
      481 ttggataact gatttcattg ccgcgcaaaa ggccgcgctc gattccatcc cggcgaaggc
      541 cgtcgcgcaa gtgattgaaa cttttcaaaa ggcgctggcc gaagaccggc agatttttgt
      601 cttcggcaac ggcggcagcg cggccaatgc ctcgcatttt gtcaccgacc tcggcaaggg
      661 ctcgtcggac aagattggcc gccgtttccg cgtctctgcg ctgaacgata atgtgagctg
      721 gatgaccgcc atcggaaatg attacgcgta tgacgaagtt ttcgtccggc aattgatgaa
      781 ctacgggcgt cccggcgaca tcgtgctggc gttgagcgtg agcggcaatt caccgaacgt
      841 ggtgaaggcg ctcgaatggt cggtggcgaa cgggttgcat gtcatcgcgc tcgtcggagg
      901 caagcgcggg cgcatggctg aaatagcgca ccaaagcatc gtgatcgatt cgacccatta
      961 tggacgcgtc gaggacgcgc aaatgggcat ctgtcacatg atttgctacg cgttcatgga
     1021 aaatccggaa atcggaaaag tgtagttggt cggatgttcg cgcgccgcgt tcaccggcga
     1081 aaacggagcg cggctcgatg agccgcagca acgcgcgagg aacaggacat gccggactaa
     1141 ttcatatcat acactcgtac ctcccacatt gctgcgggtc gccgacccgc gctccggggc
     1201 aatgcgcgga tgcggccgtt tctcccctga atttaaattt tccttattgt ctttttaatt
     1261 tcgagcatgg agcatccatc gcgtggggat gaattttttt caacaacagg agcaggcgcg
     1321 gcgcaggtcg aagcggctga taattttatt tttcctgtca gtgctcgtga tgatggtcgt
     1381 cgtctatgcg attgtcacac catgcgtttt ggcattccgg gaatttcgcc acgagcatct
     1441 gggtgtgttt ccatcgctga tcatgatgtg gttcgggatt ccgatggcgg tgatggaggc
     1501 gatcacggaa ccggccaaat tcttgaaaga cgcgtggcgt ccctggatgt tcacgggaat
     1561 ttcagtcggc acgttgctgg tggtcgcatc gggcagcatt ttcaagatga tacaactgtc
     1621 gcgcggcggc tcggtggtgg cggaattgct cggcggacgt ttgctcggcc aacgcccgtc
     1681 ggacgtggac gaattgaaat tgcgccatgt ggtggaggag atggccatcg cttcaagcat
     1741 gccgatgccg caaatctatg tgctcgacaa cgagcgcggc atcaattcct tcgccgccgg
     1801 gcattcgacc agcgacgtcg ccatcggcgt gacgcgcggt tgcttgaaat tgcttacccg
     1861 cgatgaattg cagggagtcg ttgcgcacga gttcagccac atcttgaacg gcgacacgcg
     1921 gctcaacatg cgcctgatgg ggttggcgca tggaatgttg tttccgagca tccttgggcg
     1981 gatgcttgtc catgaatgga cggccgaaga tggtctggcg acttcgattt tcgacaagcg
     2041 gccgttcgcc gccacaccat ttttagtggt gggtttcgcc ttgcaattga cgggatggat
     2101 tggactaccg tttgtaagag tgctgaagag cgcgatttgc cgcgaacgtg aatggctggc
     2161 ggacgcttcg gcggtgcaat tcacccgcaa cccggaaggc atcgcgggcg cgctcaaaaa
     2221 aattggcgga ttgatcatgc gtggccgcct gcgcacgccg cacgccgaaa ccgccagcca
     2281 tctttatttt gtgaattgtt ccacggacgc gtttttccaa atttttgcga cgcatccacc
     2341 gctggagaaa aggatttccg cgatctaccc ctatttcgat gggcaatttc cgcacgtgaa
     2401 catgctgccc atgtccgaag gcgaacgcga cacgcgtctg gaggaatcga ttgcgacgct
     2461 ggtgctctcc gagcctgcgc aaaaaatggt ggtttccacc ggcgagaaaa tgatcacgcc
     2521 ggaacaagcg cgggaaatct cgcaaagttt ccgcagcgcg gcgcttttgc ggcaggcgat
     2581 tccgagcgat gtttcgcagg ccgcgcatca accagccgga gcgatggcat ttatttatgc
     2641 gctgctgctg gatgccgacg ccgaagcccg cgctttgcaa ttgaaggaac tgcaggcaaa
     2701 cgctcctcct gatatttaca agcaagcaat cggattggcg gcccaaaccg ggactctcga
     2761 tgaacgcgca aagctggcgt tgattgacat ggcattgccg gccttgcgcg cacttacgtt
     2821 cgaccaatat caagaaatga tgcgcaacac ggaggcattg atcgaaagcg accatggcat
     2881 cgtcttgttc gaatacacga tgcagaaagt tcttgagcgg catctcagcc cgcgattcca
     2941 caaaatgccc gcgccgcgtg tggattaccg ctcgttgcag ccattggccg gggattgcgc
     3001 cgtgctgctc tccgcgcttg cgcacatgga ccaggatgat cccgcggttg cccggaatgc
     3061 tttcaccgca ggcgtggaac aacttgattt gcgcgactgc caaatttcac tgcttgattc
     3121 caagaattgc aacctcccgc aaattgacgt tgcgctccaa cgcaccgggc gcggctcgct
     3181 ttatgtgcgg cgcaacctga tgtacgcctg ctcccacacc gccgctgcga atggccgcgt
     3241 cgcaggacgt gaaattttgc ttcttcgcgc gattgccgat gctttggatt gtcccgtccc
     3301 gccgtttgtg gatgccgccg agatgcgcgc ccaatcgcag gtgaacgcgt agctgaaaac
     3361 ggaagttttc agcagttgag cgttaaggct tcgtctcgtg ccggcctttg aaggtcaatt
     3421 ccgcaatgcc cgatttgtcc aattcgccaa gaccttccgc gaccattttg tcgtattgtt
     3481 ttttggtggc cgtcgcgagc gggagattga gaccttggga cttgccgagc ttgagggcga
     3541 tgccggaatc cttcgcggca tgagcggcgg aaaaaaagca gctatgatcg cggttctgca
     3601 tgtcttcgcc gtcagtggca aggacgcgtg aattggcgcc ggtttgtgaa aaaacttcgc
     3661 gcagcatcgt caaatcgaga ccgagcgccg cgcccaggcc aagaccttcg gcaaggccgg
     3721 cggtgttgat gttcatgacc atgttcacga gcgccttcac tttcgcggct gcgccggcgg
     3781 ggccgatata tcgcaacgat gcgctcaatt tttcgaggac cggtttggcg cgttcgaacg
     3841 cttcaggctt tccgccgcac atgagataaa gcgtgccctg ccgcgcctgg gtgatgctcg
     3901 atgccatgca ggcttccagc gcttgcgcgc cggatttcgc ggccttttcc tcgactttga
     3961 catgcatgtc gggcgaaatg gtggcgcagt tgatgaacaa tttgcctttt gcgcggctga
     4021 gcagaccacc gctgtaaatt tttttcatcg cggcgtcatc ggtcacaacg gtgatgatga
     4081 tgtccgagag tttggtgacc tgtttcaatt cggtggcggc ttcgcaaccc aattcctgcg
     4141 cgagcgcttg tgccgcgggc gcgttcacgt cataaaccgc cgagacggtg taaccctgtt
     4201 ccttcaaacg gcgcgccatg ttgctgccca ttcggccgac tccgacgaat ccaattcttt
     4261 ccgacatagt tcaatccagg ttgtggtttc aagttgttgc atccagccgc cagcagcggc
     4321 gcttccgcac attggaaatc gcgagaagaa ataaacgaat tgctgcgaaa aggcacgact
     4381 ttt
//
LOCUS       DASZMM010000493         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4881193, whole genome shotgun sequence.
ACCESSION   DASZMM010000493 DASZMM010000000
VERSION     DASZMM010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4881193"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1756)
                     /locus_tag="VG754_11880"
     CDS             complement(<1..>1756)
                     /locus_tag="VG754_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HWF19962.1"
                     /translation="NEFTVNTEIIDAELKAALKNASAQRGSFREAIDDKTVRITSKNE
                     QHHRAKSEEIEVKHRNRLAWLEQAADARKKEFVKACTEREAKLIEEKENLWRNLETDW
                     QARSRPIYDALLASQNAAAKLFPPWSEKVLNDWTPQRVFEHAAKFADLEVDMTKLCEN
                     VPKETRLVLPGPSQFNVPLLLTYPEQGSILYETSNTGREGVIDSLNNIILRLLATAPP
                     GRLSFTIVDPVGLGQNFAGVMHLADYGEQLINSRIWTQSGQIEQRLGELNEHMEKVIQ
                     MYLRNEYETIAEYNEAAGNIAEKYQFLVIADFPMNFSEMAIKRLLSIATSGARCGVYT
                     LIHWDKRHPVPQDFVPEELKKAGVVISSKGNETVLSGKAFPGMTLHLESPPAPELATN
                     FIHRVGKASRDSSRVEVPFAHVVPPDNELWSEETTSELRAPIGRTGATKLQYLAIGKG
                     TRQHALIAGKTGSGKSTLFHVIITSLSLWCSPEQVEFYLVDFKKGVEFKCYAAKRLPH
                     ARVVAIESDREFGLSVLQRVDDELKRRGDLFRKLGAQDIGGYKRAGGTEPMPRSLLLI
                     DEFQELFVEDDKISQGASV"
BASE COUNT          350 a          502 c          478 g          426 t
ORIGIN      
        1 caccgatgcg ccctgcgaaa tcttgtcgtc ctcgacaaac aattcctgga actcgtcaat
       61 cagcagcaac gaacgtggca tcggttccgt gccgcccgcg cgtttgtaac cgccgatgtc
      121 ctgcgcgccc agtttgcgga ataggtcgcc gcggcgtttc aattcatcat ccacgcgctg
      181 caacacgctg agaccaaatt cgcgatcgct ctcgattgcc accacgcgcg cgtgcggcag
      241 gcgcttggcg gcgtaacatt tgaactcaac gcctttcttg aagtccacaa gataaaattc
      301 gacctgctcg ggactgcacc acaacgaaag gctcgtgatg atgacgtgga acaatgttga
      361 ttttccagaa ccggtctttc cggcgatcaa cgcgtgttgg cgcgtgcctt tgccgatggc
      421 gagatattgc aatttcgtcg cgccggtgcg gccgatgggc gcgcgcaatt cgctggtggt
      481 ttcctcgctc caaagttcgt tgtcgggagg aacgacatgc gcaaacggca cttcgacgcg
      541 gctcgaatcg cggctggcct tcccgacgcg atgaatgaaa ttcgtcgcca attcgggcgc
      601 gggcggcgat tcgagatgaa gcgtcatgcc cggaaatgcc ttgcctgaca ggacggtttc
      661 gttgcccttg ctcgagatca caacgcccgc ttttttcaat tcctcgggca cgaaatcctg
      721 cggcacggga tgacgcttgt cccaatggat caatgtgtaa acgccgcaac gcgcgccgct
      781 ggtggcgatg ctcaacaacc gtttgatggc catttcgctg aagttcatcg gaaaatccgc
      841 gatgacgagg aactgatatt tttcagcgat gttcccggcg gcttcgttgt attccgcgat
      901 ggtttcgtac tcgttgcgga gatacatctg gatgactttc tccatgtgct cgttcaactc
      961 gcccaggcgt tgttcgatct ggcccgattg cgtccagatg cggctgttga tgagttgctc
     1021 gccgtaatcg gccaggtgca tcacaccggc aaaattttgc cccaagccga cgggatcaac
     1081 gatggtgaag ctcaatcttc ccggcggcgc cgtcgccagc aatcgcaaaa tgatgttgtt
     1141 caacgaatcg ataacgcctt cgcgaccggt attcgaggtc tcatataaaa tcgagccctg
     1201 ctcgggatac gtgagcagca agggaacatt aaattgcgac ggtccgggca ataccaaccg
     1261 ggtttccttg ggaacatttt cgcaaagttt ggtcatgtcc acttcaaggt cggcgaactt
     1321 tgccgcgtgc tcgaaaacgc gctgcggcgt ccaatcgttc aagacctttt cgctccaggg
     1381 cgggaagagt tttgccgcgg cattttgcga agcgaggaga gcatcgtaaa tcggccggct
     1441 gcgcgcctgc cagtcggttt ccaaattgcg ccaaagattt tccttttctt caatcagctt
     1501 ggcttcgcgc tcggtgcagg ctttgacaaa ttccttcttc cgcgcgtccg cagcttgttc
     1561 cagccacgca agccggtttc ggtgcttgac ctcgatttcc tccgatttgg cgcgatgatg
     1621 ttgctcgttt ttcgaggtga tgcggacggt cttgtcatca atcgcctcac ggaaagaacc
     1681 ccgctgcgcg cttgcgttct tcaaagccgc tttcaactca gcatcaatta tctcggtgtt
     1741 gacagtaaac tcgttt
//
LOCUS       DASZMM010000494         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3241804, whole genome shotgun sequence.
ACCESSION   DASZMM010000494 DASZMM010000000
VERSION     DASZMM010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241804"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..2323
                     /gene="ligA"
                     /locus_tag="VG754_11885"
     CDS             284..2323
                     /gene="ligA"
                     /locus_tag="VG754_11885"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWF19963.1"
                     /translation="MTHAQAKARHAQLADELRGHDHAYYVEARPTINDQAYDRLYHEL
                     LDLEKEFPDLVTSDSPSQRVSGEPLTEFKPVAHLSPMMSLDNTYSQGEVRDFVSRVQR
                     HLPGQTLEWILEPKVDGVAINLRYENGRFICGATRGDGTTGDDVSANLKTIRSLPGQL
                     RATGKKIPALMEVRGEVFMTKTGFAKLNAERAAAGEETFANARNSAAGSLKQLDPKLV
                     AKRPLDIIVYGLGQFESTEMLTQHGVMLAWLKSVGFKTPEKTWKCHSVEELLEAINEL
                     DTLRHKFVYETDGAVIKLNSFALREKVGFTSKAPRWAIAYKYAAEQAETKLKAITIQV
                     GRTGALTPVAELEPVFLAGSTIGRATLHNEDELRRKDIRVGDTVTIEKAGEVIPAVVG
                     VVLTKRTGGEKVFEFPKNCPECGSKVARAESGEGDEGAVWRCVNPDCPAQVRGRLEHW
                     CARGAMDIEGGGEVLVAQLVGKGLVGDVADLYSLKLHEVANLERMGEKSAQNFLDGVQ
                     ASKQRDLWRLLFGLGILHVGAGVAKALGKAFATLDDVFAASQEQLNDIEDVGDVIARS
                     LVQWHGDKRNRDIIEKLRKAGLNLNSALYKPAAPAGPFAGKTFVLTGTLPTLTREEAG
                     AKIEALGGKVSGSVSKKTDYVLAGEEAGSKLDKAQKLGVKIIDEAAFLNMCKQAR"
     gene            complement(2336..>2608)
                     /locus_tag="VG754_11890"
     CDS             complement(2336..>2608)
                     /locus_tag="VG754_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19964.1"
                     /translation="GILAGWRRSCPLLETEFFPAGAREQAVRAEKVLVQPPALVRARR
                     EASCEEVRLVFAQAGFVANFFEDALAFFSHVANINQISGFGRGKLI"
BASE COUNT          607 a          725 c          756 g          520 t
ORIGIN      
        1 aattggcagt gtcttcaacg gtattggtca taatcatatc aaataagccg gtttcgcgtc
       61 tgaggaacaa attgcggcgc gagacaaggc atcagtagct taaaacttca tcgccatccg
      121 cacaagcgtt atcagagcgc gtagctggaa acgtgagttt cagttttaac tttgcggccc
      181 taatttcctt ttgtttttgc cttttcgcgg ccaatccggt tccacttcca actggcgttc
      241 atcccggcgt tcgtgcattt tattttgaaa gattgtttac cggatgactc acgcccaagc
      301 caaagcacga cacgcgcaac tcgccgatga acttcgcggc cacgaccatg cctattacgt
      361 cgaggcgcgg ccaaccatca acgaccaggc ttacgaccgg ctttatcacg aactgctcga
      421 tctcgaaaaa gaatttcccg atctcgtcac gtccgattcg ccgagccagc gcgtcagcgg
      481 cgaaccgttg acggagttca agcctgtcgc gcatctctcg ccgatgatga gcttggacaa
      541 cacctattcg cagggtgaag tccgcgattt tgtgtcgcgc gtccagcgtc atttgccggg
      601 ccagacgctc gaatggattc ttgagcccaa ggtggacggc gttgccatca atctgcgtta
      661 cgaaaatggc cgcttcatat gcggcgcaac gcgcggcgac ggcaccaccg gcgatgatgt
      721 cagcgcgaat ttgaagacca tccgcagcct gcccggccaa ttgcgcgcca ccggaaaaaa
      781 aatccccgcg ctgatggaag tgcggggcga agtctttatg accaagaccg gtttcgcgaa
      841 gttgaatgcc gaacgcgctg ccgctggcga ggaaacattt gccaacgcgc gcaattccgc
      901 cgccggttcg ctcaagcaac tcgacccaaa acttgtcgcc aagcgtccgc tcgacatcat
      961 cgtctatgga ctcggccagt ttgaatccac cgagatgctc acgcagcatg gcgtgatgct
     1021 ggcgtggttg aaatcggtcg ggttcaagac gccggagaaa acttggaaat gccattcggt
     1081 cgaggaattg ctcgaagcca tcaacgaact ggacacgctg cggcacaaat tcgtttacga
     1141 aacggacggc gcggtcatca agctcaactc attcgcgttg cgcgaaaaag ttggttttac
     1201 ttccaaggcg ccgcgttggg ccatcgccta caaatacgcg gcggaacagg ccgaaaccaa
     1261 actcaaggca atcaccatcc aggtcggccg caccggcgcg cttacgccgg tggcggaatt
     1321 ggagccggtc tttctcgcgg gcagcaccat cggccgcgca acgttgcaca acgaggacga
     1381 acttcgccgc aaggacatcc gcgttggcga caccgtgacc atcgagaaag ccggcgaagt
     1441 gattcctgcg gttgtgggcg tggtgttgac caaacggacg ggcggagaaa aagtttttga
     1501 gtttccgaaa aattgtcccg aatgcggctc gaaggttgcg cgcgcggaga gcggtgaagg
     1561 cgacgagggc gcggtctggc gttgcgtgaa tcccgattgc ccggcgcaag tccgtggacg
     1621 gctcgagcat tggtgcgcgc gcggcgcgat ggacattgaa ggcggcggcg aagtgctcgt
     1681 cgcgcaactc gtcggcaagg gattggtcgg tgatgtggcg gacctttatt cgctcaagct
     1741 tcacgaagtc gcgaacttgg agcgcatggg cgaaaaatcg gcgcagaatt ttctcgatgg
     1801 cgtccaggcc agcaaacagc gcgatctttg gcgattgctt ttcgggttgg gaattttgca
     1861 cgtcggtgcg ggtgtcgcga aagcgttggg aaaagcgttc gccacgctgg atgatgtttt
     1921 cgccgcaagc caggaacaac tcaacgacat cgaagacgtc ggcgacgtca tcgcgcgaag
     1981 tcttgtccaa tggcacggtg ataaacgaaa ccgtgacatc atcgagaaac tgcgcaaggc
     2041 cggattgaat ttgaattccg cgctttataa acccgccgcc cctgccgggc cctttgcggg
     2101 caagacgttt gttctcaccg gcacgctgcc aacgcttacc cgcgaggaag ccggcgcaaa
     2161 aatcgaagcg cttgggggca aagttagcgg cagcgtcagc aaaaaaaccg attacgtcct
     2221 cgccggcgaa gaggccggct cgaaattgga caaagcgcag aaacttggag tgaagataat
     2281 tgatgaggcg gcgttcttaa acatgtgcaa gcaggctcgt taatgggcac aaaagttaaa
     2341 tcaatttgcc tcgaccaaac ccgctgattt gattgatgtt tgcaacgtgg gaaaaaaacg
     2401 cgagcgcatc ttcaaaaaaa ttcgcaacga acccggcctg agcgaagacg aggcgcacct
     2461 cttcgcacga agcctcgcgg cgagcccgga cgagcgctgg cggctgcacg agaactttct
     2521 ccgctcgcac ggcttgttca cgcgctccag caggaaaaaa ttcggtttca agtagcgggc
     2581 atgagcgccg ccatcctgca aggatgcc
//
LOCUS       DASZMM010000495         5153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_778140, whole genome shotgun sequence.
ACCESSION   DASZMM010000495 DASZMM010000000
VERSION     DASZMM010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778140"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="VG754_11895"
     CDS             <1..1821
                     /locus_tag="VG754_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HWF19965.1"
                     /translation="RLEAKAKLQGEFESKAQRIHSISQLLKAYSLYQKDVQYVVQENK
                     VIIVDENTGRLMTGRRWSDGLHQAVEAKEGVEIERETQTLATITIQNYFRLYQKLAGM
                     TGTAETEASEFFDIYKLGVAVVPTNRPVQRKDKNDSVYKTKREKYEAALKEIQSIHNE
                     GRPILVGTISVEVSEFLSRMLKKAGIIHSVLNAKYHQQEAEIVARAGQRGAVTIATNM
                     AGRGTDIKLGTGVPDLGGLHVIGTERHESRRIDRQLRGRCARQGDPGSTHFFISLEDD
                     LMRLFGSDKIVKVMERMGLEEGQELEHPWLNRSIEQAQKRVEQHNFQQRKRTLEYDDV
                     MNKQREVIYGFRNEIINTDDVRDRLMDVMEEVVIQKVEQFTSAQEPTEWNMRQLADWV
                     NLNFPLGMPEEEIIKAAQANHEAPVAGSVFDGLTPAQFGVCNFISDSVRKAYELKVSF
                     EQPDALKTVERYTILSAIDRLWQEHLYEMDSLRYSIGLRAHGQRDPLIEYKAEAFKIF
                     EELMVTIKTEICHNIFRSASSLMAFENFLKNAPQKTTHVAPPSAFSEHQPKPGSGSDV
                     VNEATAAMEKAKPVRSGPKVGRNDPCPCGSGKKYKHCCGK"
     gene            1869..2237
                     /locus_tag="VG754_11900"
     CDS             1869..2237
                     /locus_tag="VG754_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19966.1"
                     /translation="MQAGTTSASLTLLASTMTTQTQKRTMVVRKAVSLLFLALFLFLQ
                     TLAAVPGLHRHFHHDADQADHQCVVTLLLQGNLEAASSFVSVMLPAAQVSVIPLRPAF
                     IRVAIDYRLLPGRAPPVFPA"
     gene            2388..3176
                     /locus_tag="VG754_11905"
     CDS             2388..3176
                     /locus_tag="VG754_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWF19967.1"
                     /translation="MKNRFVKIFAVASVVIKEMCRRKDFYVLLIVIVLITGLLGSVRF
                     FNDDKIIRYLKEICLLLIWIASLVIAIGMMARQVPFEKENRTIFPLLAKPISRTEVLL
                     GKFLGCWLACGIALAGFYLFFGCVSVSREHHWHFVNYFQAATLHWAMLAILLAFVLLG
                     SLIFTAPSSNGTISFILAFGILLVGRHLNKIALQNDEPIRSVTYAIYYVIPHLEIFDV
                     RDLIIHNWPAIAWKYYAIAVAYAAVYTAIFLAAACLLYRRKALN"
     gene            3184..4113
                     /locus_tag="VG754_11910"
     CDS             3184..4113
                     /locus_tag="VG754_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19968.1"
                     /translation="MRTSKAPFLLLVLLLVICFSLATSLQRPSGLRAHDSDDVLAAMM
                     GESRRMFANHFFVKADAYFHSGFYPTIFDNQESFKTPHMAEDAGVLKGRNQGEETQFM
                     GKPRDIIEKFTRNFLPSVHTHLDEGGPQGTNNAGSDLGESQGGDVREILPWLQLSAEL
                     DPSRIETYMVTAYWLRKKMGKVDEAEAFIRKGLNANPGNPSLLFELGRLYHEDRKEIN
                     RPRNIWEMAVDKLDATPEPHTEEQNFMLMQLTLNLARLEESQANLQAALHWLERLEKV
                     SPEPEHVREEINDTKQKIAHSASQSGIHAPGSN"
     gene            4696..>5153
                     /locus_tag="VG754_11915"
     CDS             4696..>5153
                     /locus_tag="VG754_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19969.1"
                     /translation="MKTNLDQNAFVRLQVPLARRRAYWTAIALASLACGGVAAAQTAT
                     NAPVGGANTVGNSTNVTKLQETTVSAKLDQARTQILPDLGATSYTITAQHIEDIPGGE
                     NAPFNQVLLRAPGVAQDSAANGDLHVRGEHGNLQYRIDDVLLPEGLSGFG"
BASE COUNT         1177 a         1351 c         1393 g         1232 t
ORIGIN      
        1 cggctcgaag caaaggcgaa attgcagggc gaatttgaaa gcaaagccca acgcatccat
       61 tccatttcgc aattgctcaa ggcatacagt ctttaccaga aggatgtcca atatgttgtc
      121 caggagaaca aagtcatcat cgtcgatgag aacacggggc gcttgatgac gggacgccgt
      181 tggagcgatg gattgcatca ggcggtcgaa gcgaaggaag gcgtggaaat cgagcgcgaa
      241 acacagacgc tcgcgaccat cacaattcaa aattatttcc gcctttacca aaaactggcc
      301 ggcatgacgg gcacggctga aaccgaggca tctgaatttt tcgacattta caaactgggc
      361 gtcgcggtgg tcccgacgaa ccgtcccgtc cagcgcaagg acaagaacga ttccgtctat
      421 aaaaccaagc gcgaaaaata tgaggcggcg ctcaaggaaa tccagagcat ccataacgag
      481 gggcggccca ttttggtcgg cacgatttcc gtcgaggtga gcgaattcct ttcgcgtatg
      541 ttgaagaagg ctggcatcat tcattccgtg ctcaacgcca aatatcatca gcaggaagct
      601 gaaattgtgg cgcgcgccgg gcagcgcgga gcggtgacca tcgccacgaa catggcaggc
      661 cgcggcactg acatcaagtt ggggacgggc gtcccggatt tgggcgggtt gcacgtcatc
      721 gggacggaac gccacgaatc gcggcgcatt gaccggcagt tgcgcggtcg ttgcgcgcgc
      781 cagggcgatc ccggttcgac gcatttcttc atttccctgg aggacgattt gatgcgcttg
      841 tttggttcgg acaaaatcgt caaggtgatg gaacgaatgg gtctggaaga agggcaggaa
      901 ttggagcatc cctggttgaa ccgttccatc gagcaggcgc aaaaacgcgt cgagcagcac
      961 aatttccagc agcgcaaacg cacgctcgaa tacgacgacg tgatgaacaa gcagcgtgag
     1021 gtcatctacg gcttccgcaa cgaaatcatc aacacagacg acgtgcgcga ccgccttatg
     1081 gatgtcatgg aggaagtcgt catccaaaaa gtggagcagt tcacttccgc gcaggaaccg
     1141 accgaatgga atatgcggca gttggcggat tgggtgaatc tgaattttcc gctcgggatg
     1201 cccgaggagg aaatcatcaa agctgcgcag gccaaccatg aagcgcctgt tgcgggttcg
     1261 gtgtttgacg gattgacccc ggcgcaattc ggcgtttgca atttcatttc ggacagcgtt
     1321 cgcaaggctt atgaattgaa ggtcagcttc gagcaaccgg atgcgctcaa gacggttgag
     1381 cgttacacga ttctcagcgc gattgaccgg ctttggcagg agcatcttta cgagatggac
     1441 agcctgcgtt acagcatcgg cttgcgcgcg cacggccagc gcgatccgct gatcgaatac
     1501 aaggcggagg cgttcaagat ttttgaggaa ttgatggtca ccatcaagac ggagatctgc
     1561 cacaatattt tccgcagcgc atcgagcctg atggcgttcg aaaatttcct gaagaacgcg
     1621 ccgcaaaaaa cgacgcacgt cgcgccgccc agcgcgttct cggaacatca acccaaacct
     1681 ggttcgggga gcgacgtcgt gaatgaagcc accgccgcga tggaaaaagc caagccggtc
     1741 cgttcaggtc cgaaagttgg ccgcaacgat ccatgcccct gcggcagcgg caaaaaatac
     1801 aagcattgct gcggcaagtg agtagctgaa aaggtaagtt ttcagccgaa gcattgggaa
     1861 gaactcgctt gcaggctggc acgacctctg cttcattgac gctgcttgcg agcacgatga
     1921 cgactcaaac gcaaaagcga acgatggttg tcaggaaggc agtttcactg ctcttcctcg
     1981 cattgttcct attcctgcaa accctcgctg ccgttcccgg cctgcatcgt catttccacc
     2041 acgatgctga tcaagctgac catcaatgcg tggtcacttt gctgttgcaa ggtaatctcg
     2101 aagctgcatc cagcttcgtt tcggtcatgc ttccggcagc acaagtttct gtgattcctt
     2161 tgcgtcccgc cttcatccgg gttgcaattg attaccgtct cctgcctggt cgcgcgccgc
     2221 cggtctttcc cgcctgatac tccgtgcatt tgattttcac atctctcgat gtggaatcgt
     2281 gcccaaaatc aagttcatca ttgacgttcc ttcgttccgt cgtgcgttgg caatgcttca
     2341 tggtgcaact tgaaaacgtg aacatgaaat aatcggctgt ccaacaggtg aaaaacaggt
     2401 tcgtcaaaat attcgcggtc gcaagcgtcg tcatcaagga gatgtgccgg cgcaaggatt
     2461 tttacgtcct tttgattgtc atcgttttga tcaccggact gctcggttcg gtgcgttttt
     2521 ttaacgacga caaaatcatc cgctatttga aggaaatctg tctgttgctg atatggatcg
     2581 catcgcttgt gattgcgatc ggaatgatgg cacgacaggt gcctttcgag aaggaaaacc
     2641 ggacgatttt tcccctgttg gccaaaccga tttcacgcac cgaagtgttg ctgggcaaat
     2701 ttcttggttg ctggcttgcc tgtggcatcg cgctggcggg tttttatctt ttcttcggct
     2761 gcgtcagtgt cagccgtgaa catcactggc atttcgtgaa ttactttcag gcggcgactt
     2821 tgcattgggc gatgctggcg atactgctgg cttttgttct gctgggctcg cttattttca
     2881 ccgcgccttc atccaacggc acgatttctt tcattctcgc gttcggcatc ctgctcgtcg
     2941 gccggcatct gaacaagatt gcgcttcaaa atgacgagcc gatacgcagc gtgacctacg
     3001 ccatttatta tgtcattccg catctggaaa tttttgacgt gcgggacctg atcattcaca
     3061 actggccggc aatcgcatgg aaatactacg cgattgcggt tgcttatgcg gccgtttaca
     3121 ccgcgatctt cctggcagcc gcctgcttgc tttaccgccg caaggccctc aactgaaatt
     3181 ctcatgcgca cctccaaggc tccatttctc ctgctggtgt tgttgctcgt gatttgtttt
     3241 tcgctggcga caagtttgca acggccttct ggcttgcgcg cccatgactc ggatgatgtc
     3301 ttggccgcaa tgatgggcga aagccgccgg atgttcgcca accatttttt cgtgaaagcg
     3361 gacgcttact ttcacagcgg tttttacccg acgatatttg acaatcagga atcgtttaaa
     3421 actccgcaca tggccgagga tgccggggta ttgaaagggc gcaatcaggg ggaggaaacc
     3481 cagttcatgg gcaaaccgcg cgacatcata gagaagttca cgcggaattt tcttccttcc
     3541 gtccacaccc acttggatga aggcgggccg caagggacga acaatgccgg ttcggacctc
     3601 ggtgaatctc aaggcggaga cgtgcgcgaa attctgccat ggctgcaact ttcggcagaa
     3661 ctcgatccga gccgtataga gacttacatg gtcaccgcgt attggctgcg gaaaaaaatg
     3721 gggaaagtgg acgaggccga ggcttttatt cgcaaaggct tgaatgccaa tccgggcaat
     3781 ccgtcgcttc tgttcgagtt gggccgcctc taccatgagg accggaagga aatcaaccgc
     3841 ccgcgcaaca tatgggaaat ggccgtggac aaacttgacg caacgcctga gccgcacacg
     3901 gaagagcaaa atttcatgct catgcaactg acccttaact tggcccgtct ggaagagagt
     3961 caggcgaact tgcaagccgc gttgcattgg ctcgaacgcc ttgagaaggt ttcgccggag
     4021 ccagagcacg tccgggagga gataaatgac acaaagcaaa aaatcgcgca ttccgcttcg
     4081 caaagtggca ttcatgctcc cggttcgaac taaaaaataa tatattgatg aatcttattg
     4141 caaatcattt gcaattacta tattttcggt tccgtgcctg gtcaaagcac aaggcccttg
     4201 catttaatat gcggattgat taactcaaat cattcttcaa aacgtgagcg ggatattgaa
     4261 aactaagcag ttcgtacgat cggcactggc cgttttgctg tgcttcctgg ttctcctgat
     4321 ttcagtgctg gctgtcagtc ccgcgcttca tcagcgtttc catcccgatg ctgattctcc
     4381 caaccattca tgcgtcatcg agttgtttgc gaagggactg gtcagcgcgt cggctttaat
     4441 tcctgtgatt gccgcggtca tgctcctttt cagtggcgtt gacttggttc tcgaaacccg
     4501 gcgcttgccc gcgttggatt ttcgtttttc ccggggacga gggcctcctc gttgctaagg
     4561 ttgcgtccgt tttcttcaat ccatttggac ctttatattg atgcgctgcc gcgtggtttt
     4621 tgcatccagc ctttttgaaa agaaacgtga gaagtttttg aatcagaatt cgtaaccttt
     4681 ttgaactatt gaaatatgaa aacaaatctt gatcagaatg cgttcgtccg ccttcaagtc
     4741 ccgcttgcgc gccggcgcgc gtattggact gccatcgccc tggcttcgct tgcctgcggc
     4801 ggtgttgcag cagcccaaac cgcaaccaat gccccggttg gtggcgccaa tacggtcggc
     4861 aattccacga acgtgaccaa attacaggag actactgttt ccgccaagtt ggaccaggcg
     4921 cgaacccaga tacttcccga tttgggcgcg acgtcataca ccatcactgc acagcatatt
     4981 gaggacatcc ccggcggtga aaacgcgcca ttcaatcaag tgctgctgcg cgctccgggt
     5041 gtcgcgcagg attcggcggc gaacggagat ttgcatgtgc gcggcgaaca cgggaatttg
     5101 caatatcgca ttgatgacgt gttgttgccg gaaggccttt caggatttgg att
//
LOCUS       DASZMM010000496         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2128614, whole genome shotgun sequence.
ACCESSION   DASZMM010000496 DASZMM010000000
VERSION     DASZMM010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128614"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VG754_11920"
     CDS             complement(<1..395)
                     /locus_tag="VG754_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HWF19970.1"
                     /translation="MIAGHVAACYGATVALAATKPRLSALSGMKAGSKLVRWILVVTL
                     GCSLGLLLIYVEVSIHPVRIVDARNIYVANRLPVRDSGFKLCEEDLGRELTEVIEGQL
                     AAFRQDDYPKAYQFAASGLKAEMPLPSFER"
     gene            complement(726..1643)
                     /locus_tag="VG754_11925"
     CDS             complement(726..1643)
                     /locus_tag="VG754_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HWF19971.1"
                     /translation="MKTGYLIDMDGVIYRENRLIPGAAEFVRALQTTGAPFLFLTNNS
                     APTPEDLAVRLKHLGISGLSAKHFYTSALNTADFLSETHAGCTVFVIGEGGLLAALHE
                     RKIASDTIRPHYVVVGEGAASLERLAKAHECIERGARLLATNPDNWCPVSSDQTRPGA
                     GATTAYLEASSGRRAYYLGKPNGYMFHQARRKLMSLAGKELEQVVMIGDTMETDIRGA
                     FEAGMHAYLVLSGSTQFEALGDYVYQPTRVLQTVGDLVQEISTGVPADPLQSPSVTPT
                     GVHRGRSGLRHQTDIFAFHIPRPRPAMTK"
     gene            complement(1914..3008)
                     /locus_tag="VG754_11930"
     CDS             complement(1914..3008)
                     /locus_tag="VG754_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWF19972.1"
                     /translation="MELILLLLFVNLTVLVFTYTNGFHDAANAIATVVGTRVLTPRQA
                     VILGAITNFLGALSGVAVAKTIGAGLVDTNYVTTVTILCAMLGGIFWNVLTWRFGLPS
                     SSSHALIGGLLGAAFASAHNKLQVIKWSFSKLDPKTGHTTMDGLYPKVIIPMVSSPIL
                     GFIGGMLVMTLLFAIIRHMRPRLVERVFGKLQLLSATYMGWSHGFADGQKTMGIMALA
                     CYAATRNGDLKNLPPWLGFLNTPEFEIKFWVKCACGIAMAIGTYSGGWRIIATLGRKM
                     VRLRPVHGFAAEATGATLLLVTGKFGMPISTTHAITTAIMGVGCARRFSALNWTLVER
                     ILWTWVLTIPATGLLAYAMVWLTQVFGWTK"
     gene            complement(3015..3641)
                     /locus_tag="VG754_11935"
     CDS             complement(3015..3641)
                     /locus_tag="VG754_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HWF19973.1"
                     /translation="MFSIQKLFAKDDRFFELLAASAEEARTSVQALKQVLKAGTPHQS
                     LTEFHAARRKEKEISGQISELLTKISVTSLDREDIEAISRVLYKIPKTVEKFAEHYLI
                     AAAELKNVDFSRQLVLAEKAIDTVTAMIKELEKSHFETVNEHNQTLQKTEREADRLML
                     ELLKELYSGKYPPLTVIILRDLYDLLEKVVDRCRDVGNVVANVVLKNS"
     gene            3911..5092
                     /gene="pstC"
                     /locus_tag="VG754_11940"
     CDS             3911..5092
                     /gene="pstC"
                     /locus_tag="VG754_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWF19974.1"
                     /translation="MTEKTISSGKRSVGWMGVRRGHQARPLEWLAEKGIFLVSLSAIV
                     MVFLIFLFVAREALPIFLGQMNSASAQKALPVEAMDTMTPAQLQKYLDLTPKEFAAMD
                     HEALKTLMEVKVEAAKDASSNRDAAINTTSWRYLLFPYQWTGYSKPVFIWQPVSDIQK
                     YNIVPLLIGSLKATLVALLFSVPLALGAAIYVSQLTTPRKREWLKPMIELLAGIPSVV
                     LGFFALIVLASVLHSVFGYQSRLNAFVAGIALGLAIVPVVFSIAEDALTSVPRSYTQA
                     ALALGSSKWQAAWQIVLPAAIPGVFAAVVLGFGRAIGETMIVLMASGNASIVSWNLFD
                     STRTMTATIAAELAETVFGGQHYRILFMLGALLFVVTFVSNLAGELVINRLKHKLEGK
                     R"
     gene            5089..6039
                     /gene="pstA"
                     /locus_tag="VG754_11945"
     CDS             5089..6039
                     /gene="pstA"
                     /locus_tag="VG754_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWF19975.1"
                     /translation="MSAKNSRSFTAKRFDLGSFFFTGLTGLATLIILMMLAVILLNVL
                     IHGWRGLSLHFVFGGTQKDMFDVNHAGVLPMIVGTMARVILMTIFVIPVGVITAIYLT
                     EYAHNSSPFTRIIRGAVNNLAGVPSIVFGLFGLGFFISFIGHNMDALLHPHNADPVWG
                     KPAILWASLTLAVMTLPVVIVATEESLKAIPPGLREASLALGATKLETVRKIVLPQAT
                     PGILTGGILAISRAAGEVAPILFTGAAYYMANLPRSLHDQFMDLGYHVFILSTQSPNI
                     QKTEPILYATVVVLLLLTFALNIVAILIRARMRKHLRAMH"
     gene            6046..6879
                     /gene="pstB"
                     /locus_tag="VG754_11950"
     CDS             6046..6879
                     /gene="pstB"
                     /locus_tag="VG754_11950"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019591453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWF19976.1"
                     /translation="MENRPSQIAVAMPETPAPPAVAPQASPQPLIETADLSLYYGQSQ
                     ALKKISIEIREKLVTAFIGPSGCGKSTLLRCFNRMNDLIDNVRIEGRIHIAGNDINGG
                     DVDVIELRKRVGMVFQRSNPFPKSIHENIAYALRLQGIKSRSKLDAIVESSLRGAALW
                     DEVKDRLHSSALGLSGGQQQRLCIARAIAIRPEIILMDEPASALDPIATAKIEELILE
                     LKKEFTIVIVTHNMQQAARISDHTAFFYLGELIEYDRTGRIFTNPARKQTEDYVTGRF
                     G"
BASE COUNT         1502 a         1943 c         1869 g         1569 t
ORIGIN      
        1 cgctcaaagg atggcaacgg catttccgct ttcaaacccg acgcggcgaa ttgataggct
       61 ttgggataat catcctgacg aaacgcggcc aattgtcctt cgatcacttc agtcaactcc
      121 ctgcccaaat cttcctcgca aagcttgaac cccgaatccc gcacaggcag gcggttcgcg
      181 acgtaaatat ttcgggcgtc aacaatgcga acgggatgaa tggacacctc cacgtaaatc
      241 aagagcagtc caagactgca cccaagcgtc accaccagaa tccaacgaac tagcttcgaa
      301 ccggccttca taccactaag cgccgacaaa cgcggcttcg ttgccgcaag cgcaacagtt
      361 gcaccatagc acgccgcgac atgtccggca atcattgtga tgaagtgcca ccatttggca
      421 tctcacaatc atggtttaaa ttagcacatg gcagaaaagc gcaaacaaac tcccgtcacc
      481 ggtggtacaa ccggccaatc cgctgaaaat gcccttgact ctgtgaacgc ggaacttaaa
      541 tcggagcgtg atgaaccacg ttcacttcgc cggcagatgg aagcgaccat cgaatccaag
      601 gcgaaaaaac ggagacgcaa cgaagctcct gtctcatcat cgttcaaacc acacggctga
      661 gggctccaaa gtttcgaccc tggcactccg ttgccagtcc gatcgctccg attttcggac
      721 atgttttact ttgtcattgc aggacgcggg cgcggtatgt gaaatgcgaa aatgtccgtt
      781 tgatggcgca agccggaacg tcccctatgg acgcctgttg gagtcactga tggactctgc
      841 agagggtccg ctggcacacc cgtcgagatt tcctgcacca aatctcctac agtctgcaac
      901 accctcgtcg gttgataaac ataatcgccc aatgcctcaa attgcgtcga gccgctcaag
      961 accaaatacg cgtgcattcc cgcctcgaac gcccctcgga tatccgtttc catcgtgtcg
     1021 ccgatcatca cgacctgctc caattccttg cccgcaaggc tcatcaattt ccttcgcgct
     1081 tggtggaaca tgtaaccgtt cggtttgcct aaataatatg cgcgccgtcc cgaactcgct
     1141 tcgaggtatg cggtcgtcgc gcccgcgccg ggacgcgtct ggtcgctgga aacggggcac
     1201 caattgtccg gatttgtcgc cagcaaacgc gcgccgcgtt cgatgcattc gtgcgccttt
     1261 gccaaacgct ccaaactcgc cgcgccttcg cccaccacca cgtaatgcgg acggatggtg
     1321 tcgctggcaa tcttgcgttc gtgcaacgcc gcgagcaatc cgccttcgcc gattacaaag
     1381 acagtgcagc ccgcgtgcgt ctcgctgaga aaatcagccg tgttcaacgc cgaagtgtag
     1441 aaatgtttcg ccgagaggcc gctaatgccg agatgcttca ggcgcacggc caaatcttcc
     1501 ggcgtcgggg cggaattgtt cgtaagaaaa aggaaaggag cgccggtcgt ttgcagcgcg
     1561 cggacaaatt ccgccgcgcc ggggatcaat cggttttccc ggtaaatcac tccgtccatg
     1621 tcaatcaagt aaccagtttt catggcagta ttaatatgtg aacgtcccca gcaaaggccg
     1681 gtggtgcgga aacagagggg gacttccgcc gggttttggc ggacgctttt aattaaacgg
     1741 ataaaaggga tttgtcaatc attcgttcct tacggtcatg tcacattttt gtgccagccg
     1801 aatgaggtga tccccttacg gccattttgc gcgggcaaca actgtgctcc cttaacgtta
     1861 aggcttcgaa tccaccgtca cagtgcgcgg atgcgccctc aagacgtgca gaatcatttt
     1921 gtccaaccga aaacttgggt cagccagacc atcgcatagg cgagcaagcc ggtggcggga
     1981 atggtcagca cccaagtcca tagaatgcgt tccacaagtg tccagttcaa cgcgctgaaa
     2041 cgacgcgcgc acccgacgcc catgatggcg gtcgtgatgg cgtgggtcgt tgagatgggc
     2101 attccgaact tgccggtgac gagcagcaag gttgcgccgg tcgcttccgc cgcgaaccca
     2161 tgcaccgggc gcaatcggac catcttgcgt cccaaagtcg cgatgatgcg ccaaccgccg
     2221 ctgtaagtgc cgatcgccat cgcaatccca caggcgcatt tgacccaaaa tttgatttcg
     2281 aattccggcg tgttgagaaa tccgagccat ggcggcaggt ttttcaaatc cccgttgcga
     2341 gtcgcggcgt aacacgcgag cgccatgatg cccatggttt tctggccgtc ggcaaaacca
     2401 tgcgaccagc ccatgtaagt cgcgctgagc aattgcagtt ttccaaaaac ccgctccaca
     2461 agccggggcc gcatgtgacg gatgattgca aacaacaagg tcatgaccag catcccgccg
     2521 atgaacccca aaatgggcga actcaccatc ggaatgatca ccttggggta gaggccatcc
     2581 atggttgtgt ggccggtctt tggatccaat tttgaaaatg accatttgat gacttgcaat
     2641 ttattgtgcg cagaggcgaa agccgcgccg agcaagccgc cgatgagcgc atggcttgaa
     2701 ctggaaggca gcccgaaacg ccaggtcagc acgttccaaa aaatcccgcc gagcatcgcg
     2761 cacaaaatgg tgacggtggt cacataattg gtgtccacca atccggcgcc gatggttttg
     2821 gcgacggcca cgcctgaaag cgcgccgaga aagttggtga ttgcgccaag gatgaccgcc
     2881 tggcgcggcg tcaatacgcg cgtgccgacg accgttgcaa tggcgttggc cgcatcgtga
     2941 aacccgttcg tataggtgaa tacgagcacg gtcaagttga cgaacagcag cagcaggatc
     3001 aattccatgc ggaatcagga atttttcaac accacattcg ccaccacatt gcccacgtcg
     3061 cggcaacggt ccaccacctt ttcaagcaaa tcataaaggt ccctgaggat gatgactgtc
     3121 aatggcggat acttgccgct gtaaagttct ttcagcaatt caagcatcaa ccggtccgct
     3181 tcgcgttcgg tcttttgcag ggtttggttg tgttcattga ccgtttcaaa gtgggatttc
     3241 tccaattcct tgatcatggc ggtgacggtg tcgatcgcct tttcagccag cacgagttgg
     3301 cggctgaaat ccacgttttt caactccgcg gcggcaatga ggtaatgttc ggcaaatttc
     3361 tcgacggtct tggggatttt gtagagcacg cgcgagatgg cttcgatgtc ttcgcggtca
     3421 agggatgtca cgctgatttt tgtcagcagt tcggagattt gtccgctgat ctccttctcc
     3481 tttcgacggg cggcatgaaa ttcggtgagg ctttgatgcg gcgtgccggc cttgagaacc
     3541 tgcttcaacg cctgcacgct cgtgcgagct tcttcggcgc tcgcggcgag caattcaaag
     3601 aacctgtcat ccttcgcgaa aagcttctga atggaaaaca tagccagcct ttctaccggg
     3661 ttcgagtgat gccgtaaaca aacttctctt ttttgcttcc gtaacacaat tgtaacattc
     3721 ctgacacggc gttgtcgtaa attgtaatta gcctgattac atgggtttgt atgcgggcaa
     3781 aatgcaagtc acgagcggaa tatgagaacg ccaggcataa aaatttgatt aacagccggc
     3841 ttgcaatcaa aatacccggc catgcgggca gcccaacgtt ttggagcggc cggttttcat
     3901 tttcaatgtc atgacagaaa agacaatcag cagcgggaaa cgaagtgttg gctggatggg
     3961 agtccggcgc ggccatcaag cgcgtccgct cgaatggctt gcggagaagg gcatttttct
     4021 ggtttcgctt tcggcgattg tcatggtgtt cttgattttc ttgttcgtcg cccgcgaagc
     4081 gttgccgatt ttccttggtc agatgaacag cgcctcggcc caaaaagcgc tgccggttga
     4141 ggcgatggac acaatgactc ccgcgcagtt gcagaaatac ctggatttga cccccaagga
     4201 attcgccgcg atggaccacg aagcgctcaa aacgctcatg gaagtcaaag tcgaggcggc
     4261 aaaggacgct tcgtccaaca gggacgccgc catcaacacg acttcgtggc gctatctgct
     4321 ctttccttat caatggaccg gttacagcaa gccggttttc atctggcaac ccgtttccga
     4381 catccaaaaa tacaacatcg tcccgctgct catcgggagt ttgaaagcga cgctcgtggc
     4441 gttgctgttt tcggtgccgc tcgcccttgg tgcggcaatt tatgtttcac aactcaccac
     4501 gccgcgcaaa cgcgaatggc tcaagccgat gattgaattg ctggcgggaa ttccgtctgt
     4561 cgtgctcggt ttttttgcgc tgattgtcct ggccagcgtg ttgcacagcg tattcggcta
     4621 tcaatcgcgc ttgaacgcat tcgtcgccgg catagcgttg gggttggcga ttgtcccggt
     4681 cgttttttca atcgcggagg atgcgttgac gagtgtgccg cgcagttaca cgcaggctgc
     4741 tcttgcgctc ggttcttcca aatggcaggc ggcgtggcaa atcgtcttgc cggcggcaat
     4801 ccccggcgtt tttgcggccg tggtgctggg ctttggccgc gccatcggcg aaacgatgat
     4861 cgttctcatg gccagcggca atgccagcat cgtttcgtgg aacctctttg attccacacg
     4921 cacgatgacc gcgaccatcg cggcggaact cgccgaaacg gtctttggcg gccagcatta
     4981 ccggattttg ttcatgctgg gcgcgttgct gttcgtcgtg acgtttgttt caaatctcgc
     5041 gggcgaactg gtcatcaacc ggctcaagca caagctggag ggcaaacgat gagcgcaaaa
     5101 aattcccgct cgttcacggc caaacggttt gatctgggct cgtttttctt cacggggctc
     5161 acgggattgg ccacgttgat catcctgatg atgctcgcgg tgattttgtt gaatgtgctc
     5221 atccacggct ggcgcggctt gtccctgcat tttgtttttg gcggcacgca aaaggacatg
     5281 ttcgacgtga accatgcggg ggttcttccg atgattgtcg gcacgatggc gcgcgtgatc
     5341 ctgatgacga tttttgtcat cccggtcggc gtcatcacgg ctatttatct gaccgagtat
     5401 gcgcacaact cgtcgccgtt cacccgcatc attcgcggcg cggtgaacaa tctcgcgggc
     5461 gtgccttcaa tcgtgttcgg cttgttcggc cttggatttt ttatttcatt catcggccat
     5521 aacatggatg cgctgcttca tccccataat gcagacccgg tctggggcaa accggccatt
     5581 ctttgggctt cgttgacgct ggcggtgatg acattgcctg ttgtcatcgt ggcgacggaa
     5641 gaatcgttga aggcgatccc gccaggtttg cgcgaagcga gcctcgcgct cggcgcaacc
     5701 aagctggaaa ccgttcgcaa aatcgtcctg ccgcaggcca cgcccgggat tttgaccggc
     5761 ggcattctgg ccatcagccg cgcggctggt gaggtcgcgc ccattttgtt cacgggcgcg
     5821 gcgtattaca tggccaatct gccgcgctcg ctgcacgatc agttcatgga cttggggtat
     5881 cacgtgttca tcctctccac gcaatcgcca aacatccaga agaccgagcc catcctttac
     5941 gcgacagtgg tggtgttgct attgttgact tttgccttga acattgtggc aatattgatt
     6001 cgcgcgcgga tgcggaagca tttgcgcgca atgcattgat accagatgga aaatcgccct
     6061 tcacaaatcg cggtcgcgat gccggagaca ccggcgccgc ccgctgttgc gccgcaagca
     6121 agtccccaac cgctcatcga gacggcggac ctttccctgt attacggcca gtcgcaggcg
     6181 ctgaaaaaaa tttcaatcga aatccgcgag aaactggtca ctgcgttcat cgggccgtct
     6241 ggctgcggca aatccacctt gttgcgatgt ttcaatcgca tgaatgacct gattgacaat
     6301 gtgcgcatcg agggccgaat ccacattgcc gggaatgaca tcaatggcgg tgatgtggac
     6361 gtgatagaat tgcgcaaacg cgtcgggatg gttttccagc gctcgaaccc gtttccaaaa
     6421 tccatccacg agaatatcgc ttacgcgctc cgtttgcagg gcatcaagag ccgttcgaaa
     6481 ctggatgcca tcgtggaaag cagtttgcgc ggcgcggcgc tttgggatga agtaaaagat
     6541 cggttgcatt cgagcgcgtt gggtttgtcc ggcggccagc agcaacgctt gtgcatcgcc
     6601 cgcgccatcg ccatccgccc ggaaatcatc ctcatggacg agccggcttc ggcgctcgac
     6661 cccatcgcca ccgcgaagat cgaggaattg attttggagt tgaaaaagga atttacgata
     6721 gtcatcgtca ctcacaacat gcagcaggcc gcgcgcatct cggatcacac cgcctttttt
     6781 tatcttggcg agttgatcga atacgaccgc accggccgga ttttcaccaa tcccgcgcgc
     6841 aaacaaacag aggattacgt caccgggcgt ttcggttgac aat
//
LOCUS       DASZMM010000497         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1611993, whole genome shotgun sequence.
ACCESSION   DASZMM010000497 DASZMM010000000
VERSION     DASZMM010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611993"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VG754_11955"
     CDS             complement(<1..1008)
                     /locus_tag="VG754_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF19977.1"
                     /translation="MRQLQEKLSIARGEQPAELLFKNAKLVNVLSGEIYPANVAVADG
                     RIIGIGAYEAEKVIDLEGSYLAPSFIDGHFHVESSMLTMPEFARAVAPHGTGAVVIDP
                     HEYANVLGLDGIRYVLESSKNLPLDFFIMLPSCVPATQLETAGARLTADDLALMIADE
                     RVAGVAELMNYPGVYLGQESELAKIRAGKGKAIDGHAPGLRGKNLNAYVLAGVRSDHE
                     STELAEAQEKLRLGMHLLLREGSTERNLATLAPLVNSHNAMNCSFATDDKLAGDLVTE
                     GHIDHCVRKAIKLGVAPIAALQIATINTARHYRLRNFGAIAPRYWADFVVFDDLKKII
                     VR"
     gene            complement(1045..1887)
                     /locus_tag="VG754_11960"
     CDS             complement(1045..1887)
                     /locus_tag="VG754_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HWF19978.1"
                     /translation="MHQKLKNSLAISISAAVLTLSFFARADELSDKGRDIFNKHQHAV
                     VTVQLVQKIAANGKYQARESKQEVTGTVLDSSGLTVLALSSCDPSELTRRMTSDSSLE
                     IELSDIKIMLEDGTEIPADIVLRDKDLDLAFIRPKTKTAAPMDAIELGKASHAQVLDQ
                     LITLNRLKRAAGRAFSASVERISAVVQKPRTFYIPDGSQTDTTLGSPAFALDGNVVGL
                     VVMRAVSAQGAASMRDCLTAIILPAEDIAKAASQAPTEVKPEEPKKDASAKTDAPKVP
                     AKAP"
     gene            complement(1916..3961)
                     /locus_tag="VG754_11965"
     CDS             complement(1916..3961)
                     /locus_tag="VG754_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWF19979.1"
                     /translation="MAAAIVATVLFFSMGDAPAVEQNTPMALRQQIDWAIARVKPALV
                     RIRVVSTEFREGREVKVQEVGSGAIISKDGYIITNHHVAGHAARMFCTLWNREEIEAE
                     LVGTDPLTDISIIKLKPATSREFKFVGFGDSSKMRVGDSVLAMGSPMALSQSVTLGIL
                     SNTEMVMPRFWGSHGAFRLDGEDVGALVRWFAHDASIYGGNSGGPLVNLRGEIIGINE
                     ISFGLSGAIPGNLAKSVATALIADHHVERSWLGVDAQPMFKQSSEEHGILISSVVESS
                     PAAMAGIKPGDILLRLGKTPTDVHFDEQMPDFMWLISTLPLGKDIPAVVKRDGKELTL
                     TLTPVERGQIFLPQQEVKSWGLVARNLSFLLAKEMKRTNLDGVLVTSIRQGGPAGECK
                     PTIESGDVIVEVNHQPVKSVEQLEALSQKLIEGKSDRVPVIAVFERKAEREMTVVRVG
                     VQDLKDPGLEVSKAWLPVETHVISRDIAELLNQPSLKGFYLTRVYPDSTAEKAGLKPG
                     DFITAVDGEPLTASGPEHEDDLSTLIRQYDIGKTVDLTVLRDKQKLKITVELERSPRL
                     EREMKKYRNDDFEFTARDICFFDAADEQWSGEQRGALVEEVRSGSWAELASLYPGDLI
                     LEVDGEPITSVESLRATMEKIASAKKSVVVMKVLRGIHTSYLQFEPNWKPEIKLGSN"
     gene            complement(4373..>4645)
                     /locus_tag="VG754_11970"
     CDS             complement(4373..>4645)
                     /locus_tag="VG754_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HWF19980.1"
                     /translation="LYQKNLLVKNQMPVGKHTVDLRKITCPVLNIMAQNDDLVPCTQS
                     APFNDLVGSRDRKSIVVPAGHIGLAVGGKAQKEAWPEACRWLAQRS"
BASE COUNT          959 a         1347 c         1292 g         1047 t
ORIGIN      
        1 gcgaacgatg atttttttca agtcgtcgaa cacgacgaag tcggcccagt aacgcggcgc
       61 aatggcgccg aagttgcgca ggcgataatg tcgcgcggtg ttgatggtgg cgatttgcaa
      121 cgcggcgatg ggcgcaacac cgagcttgat cgccttgcgc acgcaatggt caatgtggcc
      181 ctcagtcaca aggtcgcccg caagcttgtc gtccgtggcg aaggaacaat tcatcgcgtt
      241 gtgcgaattg acgagcggcg cgagcgtggc gagattgcgt tcggtgctgc cttcgcgcaa
      301 caacaggtgc attcccaaac gcaatttttc ctgcgcctcg gcaagctcgg tggattcgtg
      361 gtcggaacga acgccggcaa ggacatacgc attcaggttt ttgccgcgca aaccgggcgc
      421 gtgaccgtca atggccttgc cctttcctgc gcggattttg gccagctcgc tttcctggcc
      481 aagataaacg ccgggataat tcatcaattc cgccacgccc gcaacgcgtt catcggcgat
      541 catcagggcg agatcgtccg cagtgagccg cgcgccggca gtttcgagtt gcgttgcggg
      601 aacgcaagac ggaagcatga tgaaaaaatc gagcggcaaa ttcttgctcg attccaaaac
      661 ataacggatg ccgtcgagac caaggacgtt tgcgtattcg tgcgggtcga tcaccaccgc
      721 gcccgtgccg tgcggcgcga ccgcgcgcgc gaattcgggc atcgtgagca tcgagctttc
      781 gacatggaaa tggccgtcaa tgaaactcgg cgcaaggtag gaaccctcca aatcaatcac
      841 cttctcggcc tcgtaagcgc cgatgccgat gatgcgcccg tccgcgacag ccacattcgc
      901 gggataaatt tcgccgctga gcacgttgac cagcttcgcg ttcttgaaca gcagttcggc
      961 aggttgctcg ccgcgggcaa tgctcagttt ttcctgcagt tggcgcacgt gatcaaatac
     1021 cctttcaccc aaaacccaaa gttcctaagg agctttcgcc ggcaccttgg gagcatccgt
     1081 ctttgcagac gcgtccttct tcggctcttc cggtttcact tcggtcgggg cttgggaagc
     1141 ggccttggcg atgtcctcag ccggaaggat gatggcagtg aggcaatcac gcatgctcgc
     1201 cgcgccttgc gcgctcacgg cgcgcatcac gaccaatccc acgacatttc catccaatgc
     1261 gaacgcgggc gaaccaaggg tcgtgtcggt ctgcgaaccg tcgggaatgt aaaacgtgcg
     1321 cggtttctgc acgacggcgc tgatgcgttc caccgatgcg gaaaaggcgc ggcccgcggc
     1381 gcgtttgaga cggttcaagg tgatcaattg atcgagcacc tgcgcatgag aggctttgcc
     1441 caattcaatc gcatccatcg gcgcggccgt ttttgttttg ggccggatga aggcaaggtc
     1501 aaggtccttg tcgcgaagga caatgtccgc ggggatttca gtcccgtctt ccagcatgat
     1561 cttgatgtcg ctaagttcaa tctcgagcga cgaatcggac gtcatgcgcc gcgtcaactc
     1621 ggaagggtcg caagatgaca gcgcaagcac cgtcaggccc gatgaatcca gcacggtgcc
     1681 ggtcacttcc tgcttggatt cgcgcgcctg atatttgccg ttggcggcaa ttttctggac
     1741 aagctgcacg gtgacgacgg cgtgctggtg tttattgaaa atatcgcggc ccttgtcaga
     1801 caattcgtcg gcccgcgcaa agaatgacag cgtcagcacg gcggctgaaa tggaaatggc
     1861 aaggctgttt tttaattttt gatgcatgaa tgtgctttcc gttaacgttt cttttttaat
     1921 tcgaccctaa tttgatctcg ggtttccagt tcggttcaaa ttgcagatag gacgtgtgaa
     1981 tgccgcgcaa cactttcatc acgacgacac ttttcttcgc ggaggcgatt ttctccatgg
     2041 tcgcgcgcag gctttcgacg cttgtgatcg gctcgccatc cacctcaagg atgagatcgc
     2101 cgggataaag cgaagcaagc tcggcccaac tcccggacct gacctcctcg accagcgcgc
     2161 ctcgttgctc accgctccat tgctcgtccg ctgcatcgaa gaaacagatg tcgcgcgccg
     2221 tgaattcaaa atcgtcattg cgatatttct tcatctcgcg ttcgagacgc ggcgaacgct
     2281 ccagttcgac agtaattttc aatttctgtt tgtcgcgcaa caccgtcaaa tcgactgttt
     2341 tgccgatgtc atattggcgg atgagcgtcg aaagatcgtc ttcatgttcg gggccggacg
     2401 cggtcaacgg ttcgccatcg acggcggtga tgaaatcgcc cggtttcaat ccggcctttt
     2461 ccgcggtgct gtcgggataa acacgcgtaa gataaaagcc tttcagcgaa ggctggttga
     2521 gcaactccgc gatatcgcgg ctgatgacgt gcgtctcgac cggcaaccac gccttgctga
     2581 cttcaagtcc cgggtctttc aagtcctgca cgcccacgcg aaccacggtc atctcacgtt
     2641 ccgccttgcg ttcgaacacc gcgatcaccg gcactcgatc gctttttcct tcgatgagct
     2701 tctgcgataa tgcctccagt tgttcgactg atttcaccgg ttgatgattg acttccacaa
     2761 tgacatcgcc ggattcaatc gtcggcttgc attcgcccgc cgggccaccc tgacgaatgg
     2821 aagtcaccag cacgccatcg aggttcgtgc gtttcatttc tttcgccagc agaaatgaca
     2881 aattgcgcgc caccaatccc cacgacttca cctcctgttg aggaagaaaa atctgcccgc
     2941 gctcgaccgg cgtcaacgtc aacgtaagtt cttttccgtc gcgcttgacc acggcgggga
     3001 tgtccttgcc caggggcaat gtcgaaatca accacatgaa atccggcatc tgctcgtcaa
     3061 agtggacatc cgtcggcgtt ttgccgagcc gcaacagaat gtcacccggc ttgattcctg
     3121 ccatcgccgc cggtgaactc tccaccacgc tgctgatgag gatgccgtgc tcctcgcttg
     3181 attgcttgaa catcggctgc gcgtccacgc cgagccagct tctttccaca tgatgatcgg
     3241 caatcagcgc ggtcgccacg cttttggcaa ggttgcccgg aatcgcgccg gacaaaccga
     3301 agctgatttc gttgatgccg atgatttcgc cgcgaagatt gaccagcgga ccgccggagt
     3361 tgccgccgta aatcgaagcg tcatgcgcga accatcgcac caacgcgccc acgtcttcac
     3421 cgtccagtcg gaacgcgccg tgcgaacccc aaaagcgcgg catcaccatc tcggtgttgc
     3481 tcaggatgcc gagtgtgacg gattgcgaca acgccatcgg gctgcccatc gccagcacgg
     3541 aatcgcccac gcgcatcttg gaagaatccc caaaaccgac aaacttgaat tcgcgcgaag
     3601 tcgcgggctt caacttgatg atggaaatgt ccgtcaacgg gtctgttccg acgagttcgg
     3661 cctcgatttc ttcgcggttc caaagcgtgc agaacatccg cgcggcgtgg ccggcgacat
     3721 gatgattggt gatgatatag ccgtccttgg agatgatggc gccgctgccg acttcctgca
     3781 ccttcacttc gcgcccttcg cggaattcgg tcgaaaccac gcggatgcgc accaacgccg
     3841 gtttgacgcg ggcgatggcc cagtcaatct gctggcgcaa cgccatcggc gtattttgct
     3901 cgacagcggg cgcgtctccc atgctgaaaa aaagcactgt ggcaacaatg gcagcagcca
     3961 ttaccttcga aatttcaagt ggatgaccct tgaacattcc atcagtttgg ctccaaaccc
     4021 ctcgttgtgg caagtgctga tttggcagac aaagggtatt tgctacagcg ttagtggatt
     4081 tggttcaaaa aaatagagtg gggcttacag ccttggggcc tttgaccctc aatttgtgaa
     4141 aattcgtgca attcgtgtca ttctttttca gattttgacc acggatggac agagatgaac
     4201 atcgctggcg cttcgtgcaa aaccgcacgc caatgttaca acacctccta aatgctggag
     4261 cgcggctgtg cgcgaagcga cagccgcagc aaattgccaa agtcctgaat gttccaagct
     4321 gctgcgggtc cgtcggacac agccgcgttc cgttttctgt ttatgatgtc gtctagctgc
     4381 gttgcgcaag ccagcggcag gcttcgggcc aagcttcctt ttgcgccttg ccgccaacgg
     4441 ccaaaccgat gtgcccggcg ggaaccacga tggatttgcg gtcgcgcgag ccgacgaggt
     4501 cgttgaatgg agcgctttgc gtgcagggaa caaggtcgtc gttctgtgcc atgatgttca
     4561 acacagggca ggtgattttg cgcaagtcaa ccgtgtgctt gccgaccggc atctggtttt
     4621 tgacgagcag atttttttga taaag
//
LOCUS       DASZMM010000498         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1345800, whole genome shotgun sequence.
ACCESSION   DASZMM010000498 DASZMM010000000
VERSION     DASZMM010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345800"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..334
                     /locus_tag="VG754_11975"
     CDS             119..334
                     /locus_tag="VG754_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19981.1"
                     /translation="MPETSERSRALPGARVGDPQQCGKDSAHELVWHVLFVARCCGSS
                     SRAPFACQRVRYEFRACLVVETPGFLI"
     gene            394..1530
                     /locus_tag="VG754_11980"
     CDS             394..1530
                     /locus_tag="VG754_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWF19982.1"
                     /translation="MKKWCFLPVLIGLFLGVTGCKPSGGGDVIKVGEFASLTGKEATF
                     GTSSHEGTLLASEELDAKGGVLGKKIQLLTEDDLSKAGEPATVVNKLISRDGVVAILG
                     EVASSRSLEAAPICQEAHIPMISPSSTNPKVTETGDYIFRVCFIDPFQGTVMANFATK
                     TLKAKKVSVFTDVKSDYSIGLAKFFKQQFIANGGQIVAELDYNGGDKDFKAELTAIKA
                     ANPDAVFVPGYYTDVALICIQARQLGLTVPIFGGDGWESDQLVKIGQDAVEGTYFSTH
                     YAPDVATENSKNFVANYQKRFNGKLPDAMAALGYDSMMILADAMKRAGTTDGPKVRDA
                     LAATKDFDGVTGKTTINEKRDATKSAVILQVKNGQFKYVETVNP"
     gene            1784..2695
                     /locus_tag="VG754_11985"
     CDS             1784..2695
                     /locus_tag="VG754_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWF19983.1"
                     /translation="MTSLAQQLINGLALGSIYALIALGYTMVYGVLRFINFAHSDVLM
                     LGAFSAYFLAPAVAHIFPAESYSGALLIILLSAAVCALLGILIERLAYRPLRARPKLT
                     VLITAIGVSLLIENVCQHPAVFGAETRRFPQLMPHENFQIAGLSVDSNDVVVLVVTAL
                     LLGALWFIVQRTKTGTAMRAVAFNDRAAALMGININRIISFTFGLGSALAAVAGIFYS
                     MRAPGIDPLMGVQPGLRAFTAAVLGGIGNLPGAALGGLVLGLLETFAGGVPGLSNYRD
                     GIAFAILILILLFKPAGLLGKTQVEKV"
BASE COUNT          583 a          756 c          875 g          664 t
ORIGIN      
        1 ttgaaagatt cataaaacca agcgccacga aagcgagcaa ccacgcgcga accggtttgt
       61 ttgacgccat ttagagagca tcagcggaga ccgccgcgtt aggcaagcgg aaacgagatt
      121 gcctgaaaca tcggagcgca gccgcgcatt gcccggagcg cgggtcggtg acccgcagca
      181 atgtgggaag gacagcgcgc atgaattagt ctggcatgtc ctgtttgtcg cgcgttgctg
      241 cggctcatcg agccgcgctc cgttcgcctg tcaacgtgtc cgttacgagt ttcgcgcgtg
      301 tttggtggtt gaaactcccg gttttcttat ttagtttaag gccccggcaa tccggttggc
      361 ggatttgcca gcaattttgg cccaactttt actatgaaaa aatggtgttt cttaccggtc
      421 ctgattggtt tgtttttggg tgtcaccgga tgcaagcctt ccggcggcgg ggatgtcatc
      481 aaagtcggcg agttcgcgtc gctcacgggc aaggaggcga cgttcggcac ttcatcgcat
      541 gaaggaacgc tgctggcgtc cgaggaactc gatgccaagg gcggcgtgct cggcaaaaaa
      601 atccagttgc tcactgagga cgatctttcc aaggcgggcg aacccgcaac cgtggtgaac
      661 aaattgattt cgcgcgacgg cgtggtggca atccttggcg aagtcgcttc gagccgttcg
      721 ttggaagccg ctcccatttg ccaggaagcg catatcccga tgatttcgcc gtcctcgacc
      781 aatcccaagg tgaccgagac gggcgattac attttccgcg tttgtttcat cgacccattc
      841 cagggcacgg tgatggcgaa tttcgcgacc aagacgctca aggcgaaaaa ggtctcggtg
      901 ttcacggatg tgaagagcga ttacagcatc ggtttggcga aatttttcaa gcaacagttc
      961 atcgcgaacg gcgggcagat cgtggcggaa ttggattaca acggcggcga caaggatttc
     1021 aaggcggagt tgaccgcgat caaggcggcg aacccggacg cggttttcgt tcccggttat
     1081 tacacggatg tggcgctgat ttgcatccag gcgcggcagc ttgggttgac cgttccgatt
     1141 tttggcggcg acggatggga atcggaccag ttggtgaaga tcggccagga cgcggtggaa
     1201 gggacttatt tctcgacgca ttacgcgccg gatgtggcga cggagaacag caagaatttt
     1261 gtcgcgaatt atcagaagcg tttcaacggc aaactgcccg acgcaatggc ggcgcttggt
     1321 tatgattcga tgatgatttt ggccgatgcc atgaagcgcg cgggcacgac ggatggcccg
     1381 aaggtgcgcg acgcgcttgc cgcgacgaag gattttgacg gtgtcaccgg caaaacgacg
     1441 atcaacgaga agcgtgacgc gacaaagtcg gccgtgattt tgcaggtgaa aaacggacag
     1501 ttcaaatatg tcgaaaccgt gaatccatga tggaaaaacg agaggcgtca gaatcattgc
     1561 aagctgaagg cttgctagaa attagccggg ggctgagcgc agcgacgccc ccggtaaatg
     1621 attctcaaga tgttccaccc cggaggggtg gcagaggcgg taaaaaattc tttttagctt
     1681 gcaccctttc agggtgcatt tatttctccc gtccttccgg gggtatcgct gcgctcaacc
     1741 cccggctaat ctcttcgacc ccttcggggt cgcgaactcc tgcatgactt cgcttgccca
     1801 gcaactcatc aatggacttg cgctcggttc catctatgcg ttgatcgccc tcggttacac
     1861 gatggtttat ggcgtgttgc ggttcatcaa ttttgcgcac agcgacgtgc tgatgctcgg
     1921 ggcgttctcc gcctattttc tcgcgccagc cgtggcgcac attttcccgg cggaatccta
     1981 ttccggcgcg ttgctgatca tccttctctc ggcagcggtc tgcgcgctgc tcggcatctt
     2041 gattgaacgg ctcgcgtacc gtccgttgcg cgcgcggcca aagttgacgg tgctcatcac
     2101 cgcaatcggc gtgtcgctgc tgattgaaaa tgtttgccag catcctgccg tgttcggagc
     2161 ggagacacgg cgctttccgc aactcatgcc gcacgaaaat ttccagatcg ccgggttgtc
     2221 cgtggacagc aatgacgtcg tggttctcgt tgtcaccgcg ttgttgctgg gcgcgctttg
     2281 gttcatcgtt caacgcacca agaccggcac ggcgatgcgg gccgtggcgt tcaacgatcg
     2341 cgccgccgcg ctcatgggca tcaacatcaa ccgcatcatc agcttcacgt tcggtttggg
     2401 ttcggcgctg gctgcagtgg cgggaatttt ttattcgatg cgcgcgccgg gcatcgatcc
     2461 gctgatgggt gtgcaaccgg gattgcgcgc gttcacggcg gcggtgctgg gcggcatcgg
     2521 caatctgccg ggcgctgcgc tgggcggatt ggttctcgga ttgctggaaa catttgccgg
     2581 cggcgtgccg ggattgtcga attaccgcga cggcatcgcg ttcgcgattt tgattttgat
     2641 tctgctgttc aaacccgccg ggctgctcgg caaaacgcag gtggaaaaag tgtgatgcgg
     2701 gacttggaaa catcgaacat ccaacatcga acgctgaacg ctgaaagaaa aagcaggcgc
     2761 ctcctgacaa ttcagtgttc aatgttcgat gttcagtgtt ggatgttttt gttctgcgtt
     2821 ccagaaacca tcgaaaggaa cttcaaatga tccccggcgc gaaacgatgg ttgttggt
//
LOCUS       DASZMM010000499         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4883427, whole genome shotgun sequence.
ACCESSION   DASZMM010000499 DASZMM010000000
VERSION     DASZMM010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4883427"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VG754_11990"
     CDS             <1..1692
                     /locus_tag="VG754_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF19984.1"
                     /translation="TGTIFAIVVVPVLASFAFKGLKIAKKEAWIVRWLVRLYRPALDF
                     ALKARWTVFGAAMLLLLIGGIVFHFLGSEFLPKLDEGDLWVRTFVPQSISPSEAAKLT
                     QRVRQKLASFPEVRYVVNQIGRPDDGTDVNGWDVTEYSVGLRPREEWTTAHNRDGLCE
                     AMARKLKEIPGIDSQFSQYIEDNVNEAVSGVKSELAIKLYGDDPLKLQTYADQIVDIV
                     KNVPGAKDVGTDLLMGQPQIQITIDRAAIARYGIAVQDLQNVVATAMGGQTATQVMEG
                     ERSFDLVLKLSPQSVADLISIRNIPVFGSNGERVTLGMLASVDIKPGLTRIIREENER
                     RTHIKLSTRDRDLDSVVADAQSKVNAAVHLPPGYRLEWTGSFESEQRAEKRLMVIIPL
                     TILAIFFLLYIAFDSPMLAILIVLNVPFAATGGLMALPLAGLNLSVAALVGFIALFGI
                     SIQNCVILVSRIRELRLNEMTLKDAIREGAVSRVRPMVMTALMAMLGLLPMAISKGVG
                     AETARPFAVVIIGGLITGTVLTLFILPLLYPYFEPKRVTAKDVVVVEPQPGTSPA"
     gene            1788..3080
                     /locus_tag="VG754_11995"
     CDS             1788..3080
                     /locus_tag="VG754_11995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF19985.1"
                     /translation="MPETSVVILGAGVAGLSAANELAQAGVPMFVLEARQRVGGRIFT
                     VTDSAAMPVELGAEFIHGRAPELWQIIQSARLPVHEVPERHLACRDSDSKLTDMDGFW
                     EGFERIIGKIDLKQRDEPFSIFLSHLRDVPSDWKKWANDFVEGFDAAHTERISIHALA
                     REESYSERIEGDHSFRIATGYSQIVEWLARQLELKAVPVHCGMKVETVRWKQGGVTIQ
                     ARDAGGRIRRFQAARAVITLPLGVLKSGDVQFEPEVKEKRQAIEGLEMGEVVKVNLRF
                     RSRFWPEQNFGFIHACDDWFPTWWSHEEAGLLTGWAGGPKAERMAGMDRDHIIARALD
                     AVAGIFKVPKSKVKNALDMAWFHDWTADPFSRGAYSYIPAGMMEAQQHLSWPVRETLF
                     FAGEAATLDGQLGTVHGAIGSGKRAAHEVLQSMTKAHG"
     gene            3201..>3771
                     /locus_tag="VG754_12000"
     CDS             3201..>3771
                     /locus_tag="VG754_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF19986.1"
                     /translation="MTLINPQAAREVYKKIAGNIAKIMQGQSAATRKLLAALASGGHA
                     LLEDFPGTGKTTLAKVLARSIDASFKRIQFTPDLLPSDILGVSIFNQQDHRFHFHQGP
                     VFTNILLADEINRASPRTQSALLEAMGESQVSVDGEHRPLSELFFVIATQNPVEFRGT
                     YPLPEAQMDRFAMQFKFGYVQPMEEVAILT"
BASE COUNT          815 a          971 c         1112 g          873 t
ORIGIN      
        1 acaggaacca ttttcgcaat cgtggtggtg ccggtgctgg cgagcttcgc gttcaaggga
       61 ctcaagatcg ccaagaaaga agcatggatc gtgcgctggt tggtgcggct ttatcggcct
      121 gcgctggatt ttgcgttgaa agcgcgttgg accgtttttg gcgcggcgat gcttctgctg
      181 cttattggcg gaatcgtttt ccattttctt ggcagtgaat ttctcccgaa attagatgaa
      241 ggcgacctat gggtgcgcac gtttgtgccg cagtccattt cgccgtccga ggccgccaag
      301 ctcacgcaac gcgtgcgcca gaagctcgct tcgtttcctg aagtgcgcta cgtcgtcaac
      361 cagattggcc ggcccgacga tggcaccgat gtcaatggtt gggacgtcac tgaatacagc
      421 gtcggcttgc gaccgagaga agaatggacc accgcgcaca atcgcgatgg actttgcgaa
      481 gcgatggcgc gcaaattgaa agaaataccg ggcatcgatt cgcaattcag ccagtatatc
      541 gaggacaacg tcaacgaagc tgtctccggc gtaaaaagcg agctggccat caaattgtat
      601 ggcgatgatc cgctcaagct gcagacgtat gcggaccaga ttgtggacat cgtaaagaac
      661 gttcccggcg cgaaagatgt ggggactgat ttactgatgg gccagccgca aatccaaatc
      721 accattgatc gcgcggccat cgcgcgctac ggcattgcgg tgcaagattt gcaaaacgtg
      781 gtggccactg ccatgggcgg ccagacggcc acgcaagtga tggaaggcga acgcagtttt
      841 gacctggtgt tgaaactgtc tccccaatcg gtggcagacc tcatctccat ccgcaacatt
      901 cccgtctttg gttcgaacgg cgagagggtc acgctgggga tgttggcgtc cgtggatatc
      961 aagccgggtt tgacgcgcat catccgggag gaaaatgagc ggcgcacaca catcaaactt
     1021 tccacgcgcg accgtgattt ggattccgtt gtcgccgatg ctcaaagcaa ggtgaacgcc
     1081 gccgtgcatt tgccgccagg ttatcgtttg gaatggaccg gctcgtttga aagcgaacag
     1141 cgcgcagaga agcgcttgat ggtcatcatt ccgcttacca ttctggccat atttttcctg
     1201 ctctacatcg ctttcgattc accgatgctt gcgatattga ttgtgttgaa tgtgcctttt
     1261 gcggcgacgg gcgggttgat ggcattgccg ttggctgggt tgaatttgtc ggtggcagcg
     1321 ttggttggat tcatcgcgct ctttggcatt tcaatccaga actgcgtcat cctggtttcg
     1381 cgcatacgcg aattgcggct aaacgagatg acgctgaaag acgccatccg ggaaggggcg
     1441 gtttcgcgtg tgcgtcccat ggtgatgact gcgttgatgg caatgctggg tctgttgcca
     1501 atggcgattt caaaaggagt cggtgcggaa acggcgcgtc cgtttgccgt ggtcatcatc
     1561 ggcggactga tcaccggcac cgtcctgaca ctgttcatct tgccgttgtt atatccatat
     1621 ttcgaaccga aacgggtcac ggcaaaggat gttgtggtgg ttgaacccca accgggaacg
     1681 tctccggcgt gattcagggc atttagttga aaactcacgt tttcagctac aggttgatta
     1741 cggagagtcc cccatagctg cacgcaagaa aatgggcaca ttattttatg ccggaaactt
     1801 cggtcgtgat tttgggcgcg ggtgtggccg gactttccgc agccaatgaa ctggcgcagg
     1861 ccggtgtgcc gatgttcgtt ttggaagccc gtcaacgggt tggtggccgc attttcaccg
     1921 tcactgactc agcggcaatg ccagtggaat tgggcgcgga attcatccat ggccgcgcgc
     1981 ctgaattgtg gcaaatcatc caatcagccc ggttgcccgt ccatgaagtg cccgaacgtc
     2041 atcttgcctg ccgcgactcg gattcgaaac tgaccgacat ggacggattt tgggaaggct
     2101 tcgagcgcat catcggaaag atcgatctca aacaacgcga cgagcctttc tcaatttttt
     2161 tgtcccactt gagggatgtt ccatccgatt ggaaaaaatg ggcgaatgat ttcgtggaag
     2221 gctttgacgc ggcgcacaca gagcggatca gcatccatgc gctggcgagg gaggaaagct
     2281 attccgagcg cattgagggc gaccactcgt tccgcatcgc gaccggttac agccaaatcg
     2341 tcgagtggct ggcgcggcaa ttggaattga aagctgttcc ggtgcattgc ggaatgaaag
     2401 tggaaaccgt gcgttggaaa cagggcggcg tcacaatcca agctcgcgac gcgggcggcc
     2461 gcatccggag attccaagca gcgcgcgcag tcatcacact gcctcttggc gtcctgaaat
     2521 cgggcgacgt gcaatttgaa cccgaagtga aggaaaagcg gcaggccatc gaaggtttgg
     2581 agatgggcga ggtggtaaag gtgaatttgc gatttcgctc acgattctgg ccggaacaaa
     2641 actttggatt catccatgcc tgcgacgact ggtttccgac gtggtggtcg catgaagaag
     2701 cgggtttgtt gaccggctgg gctggcgggc cgaaagccga gcggatggca ggcatggacc
     2761 gcgaccatat tattgcccgc gcgcttgatg ctgtcgctgg aattttcaag gttccaaaga
     2821 gcaaggtgaa aaatgcgctc gacatggctt ggttccatga ttggactgcc gatccgtttt
     2881 cgcgcggcgc ttacagttac attccggccg gcatgatgga agcgcagcaa catttgtcct
     2941 ggccagtccg ggaaactctc ttttttgccg gagaagccgc cacgttggac gggcaacttg
     3001 ggacggtgca tggcgccatc ggcagcggca aacgcgcggc gcatgaagtc ttgcaatcca
     3061 tgaccaaagc gcatggatga tgttgcaggc gaacaggtta tcacgagaac attctttggc
     3121 agcttttttg tcacgcctgt agattctcct gtattttttt gggtgttctg aattatagtt
     3181 tacccaagaa aagccttaaa atgactctca tcaatcctca ggctgcgcgc gaggtttaca
     3241 aaaaaatcgc cggcaacatc gccaaaatta tgcaaggcca atcggccgcc acccgcaaat
     3301 tgctggccgc attggccagc ggcggacatg ccttgcttga ggattttccg ggtactggaa
     3361 agaccaccct tgcgaaagtg ctggctcgct cgattgatgc atcgttcaaa cgcattcaat
     3421 tcacgcccga ccttcttcca tcggacattc ttggtgtctc gatcttcaac caacaggacc
     3481 atcggtttca ttttcatcaa gggccggttt tcacaaacat tctcctggcg gatgaaatca
     3541 atcgcgcttc gccgcgcacg caatcggcgc tgctcgaagc gatgggtgaa agccaggtga
     3601 gcgtggatgg cgagcatcgt ccgctttcgg aattgttctt cgtcatcgcg acgcagaacc
     3661 cggtggaatt tcgcggcaca tacccgttgc cggaggcgca gatggaccgg ttcgcgatgc
     3721 agttcaagtt cggctacgtg cagccgatgg aagaagtcgc catcctcacg g
//
LOCUS       DASZMM010000500         4461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_514738, whole genome shotgun sequence.
ACCESSION   DASZMM010000500 DASZMM010000000
VERSION     DASZMM010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514738"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VG754_12005"
     CDS             <1..579
                     /locus_tag="VG754_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWF19987.1"
                     /translation="WGRDCYCIGAANLSLSGTCGKRFSDWLAPGYAGILDHKYIYQEV
                     GYNLKPLDLQGAIGLVQLKKLEAIRVARKKVHACYFDTCKKYPGLIRIVTADERADVS
                     WFGFSITVTTTMFSRADLVRYLEGRGIQTRNYFSGNILFHPPYAHLGNPYDYPHACKV
                     MESTFFIGVHPAMTDKMIEYVQDALDEFLKKW"
     gene            640..1689
                     /locus_tag="VG754_12010"
     CDS             640..1689
                     /locus_tag="VG754_12010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF19988.1"
                     /translation="MQNPQNKETISPDKKKISAIIACYRDGEAVPFMHGRLTAVFKKI
                     GVDYEIIFVNDNSPDNAREVLTALARHDPKLVVINHTRNFGAQSAYTSGMRVATGDAV
                     VLLDGDLQDPPELIEAFYEKWREGYDVVYGVRVKRVATLFLQVAYKAFYRIFRAASYL
                     PMPLDAGDFSLMDRRVVDAMNSLPENNRFLRGLRSWVGFKQTGVPYVRPERMFGRSTN
                     SLLRNIAWARQAIVSFSYAPLDFITWLGFFTVTLSFIAIVIQVFLRIFLPESAPRGFT
                     TLLCFVLFLGGIQLLSISIIGAYLAHIYDEVKRRPPFIVDSIVNDPRGARQRSPGDEI
                     NGAKVGEREPAASDQ"
     gene            1700..3430
                     /locus_tag="VG754_12015"
     CDS             1700..3430
                     /locus_tag="VG754_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWF19989.1"
                     /translation="MNSIKVSDYIAEFLHAQGVTHVYEVIGGMIANIVDSIHTQKKIC
                     LVSVHHEQAASFAADAAGRASGVPGVAMATSGPGATNLLTGIGSAFFDSSPTVFITGQ
                     VARPEQKGERHVRQLGFQETDIVTMAAPITKAAWRVRTAEEVPDRLIEAFLLAQTGRP
                     GPVLVDIPMDVQWEKIIVAPPSRIKLPAAAQADAGDIQKVLDELNAARRPLILAGGGI
                     RAARALDLFRRFVTTTKVPVVYSLHAVDALPFENKRRVGMIGSYGNRWANLALGRADY
                     ILVLGSRLDVRQTGALVEAFKGDRTIVHVDCDPSEINNRVKGCRAIEADLHQFLTGAL
                     AASRDKESPDFADWLAELAQLKAQWPDTSELRTITGINPNVFMHQLSKHARAAAAFVA
                     DVGQHQMWAAQSLELGSQQVFMTSGGMGAMGFALPAAVGTALVFPGRPVVMIAGDGGF
                     QLNLQELQTVKRNRLPLKMIVINNHCHGMVRQFQESYFAKRYQSTYWGYSAPDFSGIA
                     KAYGIDSETVAEPENVEHALDKLWKDSAAPFLLQVMVDPFANAYPKVAFGQPITEMEP
                     FAKPVGMEGT"
     gene            3493..4437
                     /locus_tag="VG754_12020"
     CDS             3493..4437
                     /locus_tag="VG754_12020"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF19990.1"
                     /translation="MRRVIVTGASGFVGANVARRLLREGHEVHLLVRAAHASWHIDEI
                     RRDIRLHVADLADAQAVTKKIDEIKPAWIFHLAAQGGYSWQTDIAQIFQTNLTGTINL
                     VEACLKTGFESFVHTGSSSEYGFKDHAPAENEWIEPNSHYAIAKASATLYCRYTAQAR
                     QANITTLRLYSAYGPYEEPKRLMPNLIRHGLRGEWPPLVNPDIARDYVHVEDVCDACL
                     LAAQTRPPEFGAVYNVGTGIQTTLREVVETARRLLDIKAEPQWGSMPSRKWDSSTWRA
                     DNRKIQAELDWRPHHTFEAGLAKMIEWQRNRPANEGQS"
BASE COUNT         1004 a         1170 c         1260 g         1027 t
ORIGIN      
        1 tggggtcggg attgttactg catcggcgcg gccaacctgt cgctttcggg cacctgcggc
       61 aaaaggttca gcgactggct ggcgcctggg tatgccggca tcctggacca caaatatatt
      121 tatcaggaag ttggctacaa cctcaaaccg cttgatcttc aaggggccat cggactggtg
      181 cagttgaaaa aactggaagc catccgcgtg gcgcgaaaga aagtccacgc ctgctatttc
      241 gacacctgta aaaagtatcc gggtttgatc cgcattgtga cggcggacga acgcgcggat
      301 gtaagctggt tcggcttttc aatcacggtc acaacaacga tgttttcacg ggcggatctg
      361 gtcaggtatc tggaaggccg gggcatccag accagaaatt atttttccgg caacattctt
      421 tttcacccgc cctatgcgca tctaggcaat ccttacgatt atcctcacgc ctgcaaggtc
      481 atggaaagca ccttttttat cggagtccat ccggccatga cggacaagat gatcgaatac
      541 gtgcaggacg cgctggatga gtttctcaag aaatggtgaa aggctcgcaa gccacgcgtc
      601 atcaccaacc ttgaaacggc caggagagca aagcgaagga tgcaaaaccc gcaaaataaa
      661 gaaacgatca gcccggacaa gaaaaaaatc agcgcgatca ttgcttgcta tcgcgacggg
      721 gaggcagtcc ctttcatgca cgggcgactg accgccgtgt tcaaaaaaat cggggttgat
      781 tacgagatca ttttcgtgaa cgacaacagc ccggataatg cgcgggaagt cttgaccgca
      841 ttggcccggc acgatccaaa gctggtggtg atcaaccata cgcgcaactt cggcgcacag
      901 agcgcttata caagcggaat gcgtgttgcg accggagatg cggtcgtcct gcttgacgga
      961 gatttgcaag acccgccgga attgattgaa gccttttatg aaaaatggcg cgaaggatac
     1021 gacgttgttt acggcgttcg cgtcaaacgc gtggcgacct tgtttctgca agtggcctac
     1081 aaggcctttt atcggatctt ccgggctgct tcttatttgc cgatgccttt ggacgcgggc
     1141 gatttttcgc tgatggatcg ccgggtggtt gatgcgatga attcgctgcc ggagaacaat
     1201 cgttttttgc gtgggttgcg ttcgtgggtt ggcttcaagc agacaggggt gccttatgtg
     1261 cggcctgaaa ggatgtttgg caggagcacc aattccttgc tcaggaatat tgcctgggcg
     1321 cgccaggcca ttgtttcctt ttcctatgcg ccattggact ttatcacttg gctggggttt
     1381 ttcaccgtga ctctttcctt tattgccatt gtgattcagg tattcctgcg gatttttctg
     1441 ccggaatctg cgccgcgcgg ttttacaacg ctgctctgtt ttgttctttt tttgggcggc
     1501 atccaactgc tctcgatcag catcatcggt gcttatcttg cccacattta tgacgaagta
     1561 aaacgaaggc ccccatttat tgtggacagc atcgttaatg atccgagggg agcgcggcaa
     1621 cgaagtccgg gtgatgaaat taatggggcc aaggtggggg agagagaacc tgccgcgtcg
     1681 gaccaatgac cccaacatca tgaattcgat caaagtttca gattacatcg ccgagtttct
     1741 tcacgcccag ggcgtcactc acgtgtatga agtcataggc gggatgatcg ctaatatcgt
     1801 ggattcgatt catactcaaa aaaagatttg tcttgtcagt gttcatcacg aacaagcggc
     1861 ttcgtttgcg gcagacgcgg ccggccgggc aagcggcgtt cccggcgtgg cgatggcgac
     1921 cagcgggccg ggcgcgacga atttgttgac cggtatcggc agtgcttttt ttgattcgtc
     1981 accaacggtc tttatcacgg gtcaggtcgc tcgtcctgaa cagaaaggcg aacggcatgt
     2041 tcggcaactc ggatttcagg aaacggacat tgtgaccatg gcagcgccga taaccaaggc
     2101 ggcttggaga gtccgaacgg ctgaagaagt gccggatcgg ttgatcgagg catttctttt
     2161 ggcgcaaacg gggcgccccg gccccgttct ggtggacata ccaatggatg tgcaatggga
     2221 gaagatcatt gttgccccac cgagtcgcat aaaattgcca gccgccgccc aagcggacgc
     2281 gggcgacatt caaaaagtgc tggacgagtt gaatgccgcg cggcgtcctc tgattcttgc
     2341 cggcggcggg atccgggcgg cgcgcgcgct ggacttgttt cgccggttcg tcacgacaac
     2401 gaaagtgccg gtcgtctatt ccttgcatgc ggttgacgcc ctgccttttg aaaacaaaag
     2461 gcgtgtgggc atgataggca gctatggaaa ccgctgggcc aatctcgctc ttggacgcgc
     2521 ggactatata ctcgtgcttg gaagtcggct cgacgtgcga caaaccggag cgttggtcga
     2581 agcattcaaa ggagaccgga cgattgttca cgttgattgt gatccttccg agatcaacaa
     2641 tcgagtcaaa ggctgccgtg ccattgaggc cgacctgcat cagtttttga ccggcgcact
     2701 ggctgcgtca cgcgacaaag agtctccaga ttttgctgat tggctggcag aactggcgca
     2761 attaaaagcc caatggcccg acacgagcga attgcgcacg attaccggca tcaaccccaa
     2821 tgttttcatg caccagcttt caaaacacgc acgcgcggcc gcggcgtttg tggctgatgt
     2881 tgggcagcac cagatgtggg cggcacaatc gttggaactg ggttcgcaac aagttttcat
     2941 gacttcggga ggaatgggcg cgatgggatt cgcgctgccg gctgcggttg gaactgcgct
     3001 ggtgtttccc gggcgacccg ttgtgatgat agcgggtgat ggcggatttc aattgaatct
     3061 gcaagaattg caaaccgtca aacggaaccg tttgccgtta aaaatgatcg tcatcaacaa
     3121 tcattgccat gggatggtgc ggcagtttca ggaaagttat tttgcaaaac gatatcaatc
     3181 tacatactgg ggctattcgg cgcccgattt tagcgggatt gcgaaagcgt atggcattga
     3241 ttccgaaact gtcgctgaac cggaaaatgt cgagcatgcc ctcgataaat tgtggaaaga
     3301 ttctgccgcc ccatttttgc tgcaagtgat ggttgatccg tttgcgaatg cttaccccaa
     3361 ggtcgccttc ggacagccaa tcacggagat ggaaccgttc gccaaaccag tgggaatgga
     3421 aggaacttga ttggcgtttg gaaggctgcg ttgtcatttt ggttatgctc gaatcctttc
     3481 tgatcggaat tcatgaggcg tgtcatcgtc acaggggcta gtggttttgt cggcgcgaat
     3541 gttgcgcgcc gattgctgcg cgagggccat gaagtccatc tcctcgtccg cgcggcccat
     3601 gcctcgtggc acattgatga aatccgccgt gatatccggc tgcacgtggc tgacctcgcc
     3661 gatgcgcaag ccgtcaccaa aaaaattgat gaaatcaagc ccgcatggat ttttcatctc
     3721 gcggcgcaag gcggttattc atggcagacg gacattgcgc aaattttcca gaccaatctc
     3781 accggcacca tcaacctggt cgaagcctgc ctcaaaaccg gtttcgaaag cttcgtccat
     3841 accggctcgt cttccgaata cggtttcaaa gaccatgcgc ctgccgaaaa cgaatggatc
     3901 gagcccaaca gccattatgc catcgccaag gcttccgcga cgctttactg ccgctacact
     3961 gcgcaagcgc gccaggcaaa catcaccacc ttgcggctct attccgccta cggtccttac
     4021 gaggaaccaa aacgtctcat gcccaatctc attcggcacg gtttgcgcgg cgaatggccg
     4081 cctctcgtga atccggacat cgcgcgcgat tacgtccatg ttgaggatgt gtgtgacgcg
     4141 tgcctgcttg cggcgcaaac gcgcccaccc gaattcggcg cggtttacaa tgtcggcacc
     4201 ggcatacaga cgaccctgcg cgaagtcgtc gagaccgcgc gccgcctgct cgacatcaaa
     4261 gccgaaccgc aatggggctc catgccttcg cgcaaatggg atagttcgac atggcgggcc
     4321 gacaatcgca aaatccaggc ggaactcgat tggcgaccgc atcatacttt tgaggccggc
     4381 ctcgccaaaa tgattgaatg gcagcgcaac cggccagcaa acgaaggaca aagctgaaca
     4441 gcttttccct cttttcagat t
//
LOCUS       DASZMM010000501         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3759620, whole genome shotgun sequence.
ACCESSION   DASZMM010000501 DASZMM010000000
VERSION     DASZMM010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3759620"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..641)
                     /locus_tag="VG754_12025"
     CDS             complement(198..641)
                     /locus_tag="VG754_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19991.1"
                     /translation="MHPLHIGTKKTLTPRQALAISVGGLGIGMILLAVACGVFLVPFS
                     EAMAGYAIMGFFCVAFPWASLKLETRNRAVRAHNYFVYFFIAMLIVTVIAQWGTKEGT
                     PFPKWAIGLVIIAGVAAYSVMAYYAVVNFRKWLKGGFKNLDQLGM"
     gene            complement(1026..>3850)
                     /locus_tag="VG754_12030"
     CDS             complement(1026..>3850)
                     /locus_tag="VG754_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19992.1"
                     /translation="SPQAVSIQRDTAGSNTVATLVLSPLGESQIGWRPRSRDVKREKP
                     VFYAEFYQLYVPSAGVIEGAHYIALRPAQGELSELTFDVPKGATVTDVADPNASGPTP
                     LVSLWRFDPDTRKLRVNLNPPQSRPFALIIRSQVATGPLPLEQSVGLISVDNAAEQIG
                     QLGIATGNEVQLDNVTADGFSPINLEDFPSGVAQLLQGQIPGFTVHRAFRYADTKAIA
                     SIKASAVEPDVRVESQDTLSLGEDRTVLAASLTVDITRAGIFKLSFLLPQGMDVESVS
                     GQSLSHWTESKTDTGRVITLHLRGKTEGQQTFELSLTGPGIKATNGWTAPELALREAS
                     KQRGTLLIVPEQGMRLQISKRDGLTQLDPQKSGIRQKGVLAFRVLQTPWNLSLDIEQV
                     EPWIQVTSLQHAVISEAQVKVTANLQYQIENTGLKMFHVLLPTNAANVQFQGQQVADF
                     LPINGTVTNGLQMWEVKLQRRVIGQYMLQTSYQMVMPEQVAETTLRGVQAADVNLQRG
                     FLTVQAGGRLQVRPPDTMPATLQPAEWQSIPRVLQQDLTTASANFAYRLVDPAFELAL
                     KLDRREAAKLLPARINNVTFTSVISDDGVMLTHALLEMQPGDMRLLSVAIPEHGKFWF
                     AFANQNGVWPWTGAQTNSLLIPLEQPSRVGQTIPVEFFYTTEVGKGAGDMQLPAPKFD
                     LPLENITWRVFLDEKWQVKHWSGSFQLEEQQVIPCAVAVDAQGYLDTEAVAQREKTKA
                     AEQMLALGNSMLQNGDPQQARRAFQSAYGLSQGDDAFNEDSRVQLNNLKLQQALVGLN
                     LRQATTAGETATLGGKWHDLRDRKQIAYTQQDAKDILERNSADENAAFMRLAERLIQQ
                     QDAAVSSPAAIRANVPEQGRLLTFKRAVSVDSMADLRINLETAASKAAPWSVRFLVLA
                     LTLILCAGTAWAAGSFRRNEQAVSVG"
BASE COUNT          828 a         1138 c         1056 g          828 t
ORIGIN      
        1 atgaagatgg cgccacgaaa gtgctcaagc ccggccgcac ctgtgaagtg ctcgcgacca
       61 attcgctcgc ggacggtttc atgtcctcgc ccgcgatttc cggcaaggca ttttatttaa
      121 ggaccaccag caatttgtat cgcatcgagg actcggcttc caaaaattga aagaaagttt
      181 gcggttgcct gaacgactca catccccaac tgatcaaggt ttttgaaacc acccttcagc
      241 cacttccgga aatttacgac tgcgtaatat gccatcaccg aatacgccgc aacaccagca
      301 atgatgacca aaccaatcgc ccatttggga aacggcgtcc cttccttggt tccccattgc
      361 gcgatcacgg tcacgatcaa catcgcgatg aagaaataga cgaagtagtt atgagcgcgg
      421 accgcccggt tcctcgtttc cagcttgagc gatgcccagg ggaatgccac gcaaaaaaat
      481 cccatgattg cgtaacctgc catcgcttcc gaaaacggca cgaggaacac tccgcacgcg
      541 accgcgagca gaatcatccc gatgccaagc cctcccacgc tgatcgccag cgcctgccgg
      601 ggagtgagcg ttttcttggt ccctatgtgc aatgggtgca ttcgacagcg tccaaattcg
      661 tacccgcaat ccagaaactc acggatgttc aacaaggcat acgggaatat tgcgctgcgc
      721 aactctcgaa atcaaggttc aaagccgaga tacggaggcc gtaagcgcat gcgagttttt
      781 gacaaacgaa tgattgcttg cggttccatt ctcattaacc cctcgtttca acgaggggga
      841 tcagggcgca cgcaatggat ttcaaccgtt tcaacggttt gtcgggacag actgagtgca
      901 agtttccaag cacttgaaac cgttgaaaac ggttttggaa attcgcgggt ttccggtcga
      961 atcccctcgt tgaaacgagg ggttaatgag ataatccaag actgctcaaa aaacaaaggc
     1021 gtgcctcagc ctacgctgac tgcttgttcg tttcgccgaa atgaacccgc tgcccacgca
     1081 gttccggcgc acaaaatcaa cgtgagcgcc agcaccagga aacgcacgct ccacggtgcg
     1141 gctttgctcg cggcggtttc aagattgatc ctcaagtccg ccatcgagtc aacggacacg
     1201 gcgcgtttga aagtcagcag ccgcccttgt tcgggaacat tcgcgcggat ggcggcggga
     1261 ctgctcacgg cggcgtcctg ctgctggatg agccgttcag ccaggcgcat gaacgcggcg
     1321 ttttcatcgg cggaattgcg ttcgaggatg tccttggcat cctgttgcgt gtaggcgatc
     1381 tgcttgcggt cgcgcaaatc gtgccatttg ccgccaagcg tggcggtttc gcctgccgtc
     1441 gttgcctgcc gcaagttcaa gccaaccaat gcctgttgca gcttgagatt gttgagctgg
     1501 acgcgcgaat cttcgttgaa cgcatcgtcg ccctggctca aaccataggc cgactggaac
     1561 gcgcggcgcg cctgttgcgg gtcgccgttt tgcaacatgc tgttgccaag cgccagcatt
     1621 tgctcggcgg cctttgtttt ctcgcgctgc gcgacggctt ccgtgtcgag gtagccttgc
     1681 gcatccacag ccaccgcgca agggatgacc tgctgctcct ccaattgaaa cgaaccgctc
     1741 cagtgtttca cctgccattt ttcatcgaga aaaacgcgcc acgtgatgtt ctccaatggc
     1801 agatcaaatt tcggagcggg caactgcatg tcacccgctc ccttccccac ctccgtggtg
     1861 taaaaaaatt caactggaat tgtctgccca actcgggaag gttgctccaa cggaatcaaa
     1921 aggctgttcg tctgcgcgcc ggtccagggc cagacgccat tctggttggc aaacgcaaac
     1981 caaaactttc catgctccgg gatcgcgacc gaaagcaatc gcatgtcgcc cggctgcatt
     2041 tcaagaagcg cgtgagtgag catgacgccg tcatccgaaa tcaccgatgt gaacgtgacg
     2101 ttgttgatgc gcgcgggcag aagttttgcc gcttcgcgcc ggtcgagttt caaggcgagt
     2161 tcgaatgccg ggtccacgag ccgatacgcg aagttcgcgg atgccgtcgt caaatcctgt
     2221 tgcaacacgc gcgggatgct ctgccattcc gccggttgca acgtcgcggg catcgtgtcc
     2281 ggcggacgca cctgcaaccg accaccggct tgcacagtca gaaaaccgcg ctgcaaattc
     2341 acatccgccg cctgcacgcc gcgcaacgtg gtttccgcaa cctgctcagg catcaccatt
     2401 tgatacgagg tttggagcat gtattgtccg atgacgcgcc gctgcaattt cacctcccac
     2461 atttgcaaac cgttcgtgac ggttccattg atcggcaaaa aatctgcgac ctgttgccct
     2521 tgaaattgta cattcgcggc attcgtcggc agcaagacgt ggaacatttt caagccggtg
     2581 ttttcaatct ggtattgcaa attcgccgtc accttcacct gcgcttcgga aatgaccgcg
     2641 tgctgaagac ttgtgacttg gatccacggc tcgacctgct cgatgtcgag cgacaggttc
     2701 cacggcgttt gcaacacgcg aaacgccaag acgcctttct gccggatgcc cgatttttgc
     2761 gggtcgagtt gcgtgagtcc gtcgcgcttg gaaatctgca atcgcattcc ttgttccggc
     2821 acgatgagca atgtgccgcg ttgcttggag gcttcgcgca atgcaagctc cggcgcagtc
     2881 caaccatttg ttgccttgat gcccggcccg gtcaaactca attcaaatgt ttgttggcct
     2941 tccgttttgc cgcgcaaatg caacgtgatg acgcggcccg tgtccgtttt cgattctgtc
     3001 caatgactca acgattgccc gctcaccgat tcgacatcca tcccttgtgg cagcaaaaag
     3061 ctcaatttaa aaatcccggc gcgggtgatg tccaccgtga gactggcggc gagcaccgtg
     3121 cggtcttcgc cgagcgaaag agtgtcctgc gattccaccc gcacatccgg ttcgacagcc
     3181 gaagccttga tacttgcgat agctttggtg tcggcataac ggaacgcgcg atgcacggtg
     3241 aaaccgggga tctgtccttg caacaattgc gcgacgccgc tcggaaaatc ctcaaggttg
     3301 atcggcgaaa acccgtcggc ggtcacattg tccaactgca cttcgttgcc ggtggcgatg
     3361 ccaagctggc cgatttgttc agcggcgttg tccacggaaa tgaggccgac tgattgctcc
     3421 aacggcaacg gccccgtggc gacttgcgag cggatgataa gcgcgaacgg gcgcgattgc
     3481 ggcgggttta gattgacgcg cagcttgcgc gtgtcggggt caaagcgcca caacgatacc
     3541 agcggcgtcg gaccggaagc gttcgggtcg gcaacgtcag tgacggtcgc gcccttggga
     3601 acgtcgaaag tcaattcgct caactcgccc tgtgcgggac gcaacgcaat ataatgcgcg
     3661 ccttcaatca cgcctgccga cggcacgtaa agctggtaga actccgcata aaacaccggt
     3721 ttttcgcgct tcacatcgcg gctgcgcgga cgccagccaa tctgggattc gccaagcggc
     3781 gacaacacaa gcgtcgcgac ggtattgctg cctgccgtgt cgcgctggat ggaaacggcc
     3841 tgcggcgagg
//
LOCUS       DASZMM010000502        10265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4886681, whole genome shotgun sequence.
ACCESSION   DASZMM010000502 DASZMM010000000
VERSION     DASZMM010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4886681"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            590..1618
                     /locus_tag="VG754_12035"
     CDS             590..1618
                     /locus_tag="VG754_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWF19993.1"
                     /translation="MFAQFKSLFSNDIGIDLGTANSLVYVRDRGIVLREPSVVAIQAG
                     TTNVLAVGDEAKRMLGRTPGNIVAIRPMKDGVIADFEITEAMLRHFIQKVHHRKLIAP
                     RVVVAVPSGITEVEKRAVKDSATHAGAREVYLIEQPMASAIGVGLPVHEPAGNMIVDI
                     GGGTCEIAIISLAGIVFSRSLRVGGDEFDETIVAHMKRAYNLMIGERTAEEIKIRIGS
                     AFPLEQELTMEVKGRDLSAGLPKTLTIRSEEIREALQEPLSSILESVRITLERCPPEL
                     SSDLVDRGLVMAGGGALLRGIDRLVAEETGLPVHIADDPLSAVAEGTGRVLQELQFLK
                     QVTSNSKV"
     gene            1653..2459
                     /gene="mreC"
                     /locus_tag="VG754_12040"
     CDS             1653..2459
                     /gene="mreC"
                     /locus_tag="VG754_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWF19994.1"
                     /translation="MLKRPHYMALGLVGLLTLILVNLPHHTANQIKLAIGSLYLPLFG
                     LSKSSQQVLNQASDAMTPRSTLLLQNEELRRTNQELQVRAMQADALQRENDRLRQLFG
                     WEKQTTWQLRLARVVARDPANWWHTVTIDLGSRDGMRPDLPVLTPSGLVGRISRVTLT
                     SSEVVLVGSSECKVAALVKETGDTGVITGGASPLGNSSLATLSYLASSANLKPGQKVQ
                     TWGEGNVFPKGIVIGQVAEDSRQTELGYTEARVKLAADFGALEEVWVLMR"
     gene            2456..3007
                     /locus_tag="VG754_12045"
     CDS             2456..3007
                     /locus_tag="VG754_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19995.1"
                     /translation="MNWLTPILILLVAFVTVYLEATFTGVRHFLGAQIDLLPALMVYA
                     SLSASLATISVLAVCGGLWFDSLSSNPLGISILPLFLSGYLIYLRRGLILREQHFAQL
                     VLGLLASSIVPLLTLLLLLSSRQPLVVGWGTLWQWIVMSLGGCVLTPVIFRLFDGLHH
                     ALRYQPVVETSFRLDREIRRGRK"
     gene            3012..5039
                     /locus_tag="VG754_12050"
     CDS             3012..5039
                     /locus_tag="VG754_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWF19996.1"
                     /translation="MLVFDQLKKDDPQLRFLAVMVLCGMLVLLGGLWWVQVVSSRVYR
                     QSLERQSVRTIRIPPIRGRILDRDGRPLAENRPSYNVDLYLEELSKNFQKGYSAAIKD
                     ATNWLHLQLVEKEKQLGRKLTPQEKKQFALSRALRDGLQQQTRYQVISNLIDNLSARL
                     QEPVNLTPKEFETHYLKARALPMSILPNLNPVQVARFEEQSAAEPGMDLEVHSMRYYP
                     NGTIGAHLLGYLVHHNESDDNDDAEHKYNYRLPEFAGSSVGIEGVFDKELHGIAGEKS
                     VVVNYLGYKQSENIWAPPEVGQDVVLTIDLDIQKAADAALDSVKEDVHGAVVVMDVRN
                     GDVLAIASSPTYNPNHFIQRPPQETWSREWERWTNEDLQVQMNHAMQGMYAPGSIFKI
                     VVGLAALEQGVLDPHETFHSLGYYPIPGSKPIGDTAGAGEFDFDRALAKSSNPYFITQ
                     GVKPGVLAKMVALGQRLHFGERTGLLPHQEDRGYFPTPKDIASRSWVPGYTMHLSIGQ
                     DRIAVTPVQIAVMISAIANGGTVYWPRIVSRVESAGDLPAQTFPGGRVRDTLGISARS
                     LRIVHEAMSADVESPEGTGHAAAVPGLRIAGKTGTAEVEKNGHIDKASKITWFASFAP
                     VENPHYAVIAMVVHGASGGLTCAPLAHKVYEAIQESERKPHAKTGILAETR"
     gene            5042..6262
                     /locus_tag="VG754_12055"
     CDS             5042..6262
                     /locus_tag="VG754_12055"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWF19997.1"
                     /translation="MFNAIQNERPSRVDYIQLAAVLCLMLISVAFVFSATTVNDSGST
                     LAWYRQIYFKQVIWFAVGLGAAGVVCLMDYHSLTRWALVAYAASIFFLIVVLVPGIGT
                     MVYGARRWIGLAGFQFQPSEFAKLALILALAHFLSRPVDELRHRHIFWKSMGLMVLPF
                     LLIMKEPDLGSALVLIPTGFAMLYAAGTPKRYLIQLIMFGGIIGTVFLGDILFAPPKY
                     RIIKLQDYQMKRLLVYFGRNYTAYAGPGVTEAEKQRLRERELNDRYNVEQALIAVGSG
                     GLLGKGWRQGQQNALGFLPQAGKHNDFIFSVIAEEKGFMGSVTVLTLYSVILFTGIRI
                     AGQARDRLGKLLAVGVVTLIFSHVFINIGMNIRIMPVTGVPLPLLSYGGSSVLGSLIA
                     MGILQNVYMYRKAY"
     gene            6305..8230
                     /locus_tag="VG754_12060"
     CDS             6305..8230
                     /locus_tag="VG754_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWF19998.1"
                     /translation="MRFRPSSGLNPNPNRPDREALEARAEVVGEKAPQDKLYDRRHAQ
                     EIERAENIAAGLPPEGAPKPAESVPIEGDRKDAFREPNLETPAEVREEKPFAPVPVKE
                     RPQGIVETIRAAATQMIKKVHRLIKPVKRTHKEVIINAESLETRVAVMEEGKLEEFTI
                     ERTTEERLVGSIFKGKLRNLQDDLKAAFVDIGFEKNAFLHYWDIVPSNFDSGVEVVER
                     ETKRREKPRITQKDIPRLYPPGSDIIVQVTKGPIGSKGPRVTTNLVLPGRYLVLLPNS
                     DQSGISRKIENQQERQRLKKILRELTIPEGMGIIMRTVGEGQQARYFVRDLALLLEEW
                     KLIQERIKSQASATCVFQEPDLIERTVRDFLTEDVERIVVDNGKAYERMREMISKISW
                     RAAEKVKLYNESQVIFDRFNVSRQLENAFSRTVHLKSGGYIVVDETEALVAIDVNTGR
                     HKGGKDQEQAILKVNLEAAEEIARQLRLRNMGGLIVLDFIDMKHPRDRQQVYQRMKDG
                     LRRDKAKTHILPISQLGLMEMTRQRHSESVHSAVYDECPYCKGRGKVKSALTMSVEIQ
                     RKLGEILKKRSRDESDFQLRIVVHPSVLDRLRNEDEKHLIEMEKRYFGKLSFRADPAL
                     HAETFKIINVATNEEMS"
     gene            complement(8296..8787)
                     /locus_tag="VG754_12065"
     CDS             complement(8296..8787)
                     /locus_tag="VG754_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF19999.1"
                     /translation="MKMFLFTRGFKFCKPLFCICAALLLATSARAQNLRVEAKLVWGT
                     DDQHSPNPKHHLLDPVLTKRLKGSPYRWKNYFEECQRVVEIPVGQTKSKIVMSDRCTL
                     DIKNLGGDCVEVRLHGNGKPVSMHKESLKSNGLLVLGGEAPNETAWLVTIRKAAPAPT
                     ASK"
     gene            complement(8784..9305)
                     /locus_tag="VG754_12070"
     CDS             complement(8784..9305)
                     /locus_tag="VG754_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20000.1"
                     /translation="MNHDSQLKLQAFLDGELSGHELAEARDLLARDAEAQALLTELRN
                     TEAALAGNEPECQLPESREFFWSKIEREIERESEQPARPVAAQSWFAWIQRHFLPVGT
                     FALVCVLLGFVAVHSRGPGNQLGEIEVASDDMGSYTYRDQANKMTMVWLYDRNNTDST
                     VADSSDIDNVTPE"
     gene            complement(9307..9903)
                     /locus_tag="VG754_12075"
     CDS             complement(9307..9903)
                     /locus_tag="VG754_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF20001.1"
                     /translation="MKSKPDHASAGDEQLVGAAKRGDMGAFEELVARHRDKIYARAFS
                     MMRNEDEALDLSQEAWVKSWQRLKQFQGESSFGTWMTRIVINLCLDQLRRRKRQRTES
                     IEEMSEESGGVERQMPVVNVNPTERLERGELRKRIDIALGQLSHEHRTVLVLHEFEEM
                     EYKEIARTMNCSIGTVMSRLFYARRKMAVLLASLKTED"
     gene            complement(9942..>10265)
                     /locus_tag="VG754_12080"
     CDS             complement(9942..>10265)
                     /locus_tag="VG754_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HWF20002.1"
                     /translation="CLARCVIDFSGRPFLVWRGMNEMAYRKLSAKEKTQDMSSAFRFG
                     LAREFFQGFANEARCNLHLELLYGDEPHHIVEALFKAFAKAVDIACQQDARIAGQVPS
                     TKGKL"
BASE COUNT         2306 a         2702 c         2899 g         2358 t
ORIGIN      
        1 ggagttgttc tttgacatcc taaaatcgtt ccgagtctcg caagccaagt tagcggttta
       61 aagttgcgga tttcgcgaac tttcgtgttt ttcgcgggca agtcgcgtct cggttctctc
      121 atgcttgggc gtttggcgga cttcgccgaa catggcccgt ccggaaggac ctgaaaactc
      181 acgttttcag ctacgttatt ccgcttgttt cacgttgcct cggatattaa ttccgctttg
      241 ttccgcttga attcggatca aacgggcttg accgggataa accgggctaa aaaaataaat
      301 atttttttca tccggtttag ttgaaattga gtgaaatggc tcaagttgta agtggttgaa
      361 attccgcata atttcagata gtttcagata attccgcgca aaataccccc ccggggggtg
      421 gtgaatggtt gagagttgat ggttgcaaaa tgcgacggca aagcgggcgg aaacaggtct
      481 aaaatctcgg aatcccgtta tctttaaagt aatggggctg ttccttcgct aatagtcttg
      541 accgcagggg ttatggcctc ttatttttgc gccttgataa gcacaattca tgtttgcgca
      601 atttaaaagc ctgttttcca acgacattgg aatagacctt gggactgcca actccctcgt
      661 ttacgttcgt gaccggggta tcgtgctgcg tgaaccgtcc gtcgtcgcca tccaggcggg
      721 aacaacgaat gttcttgccg tgggcgatga agcgaagcgg atgttgggac gcactcccgg
      781 taacattgtg gccatccggc cgatgaaaga tggcgtgatt gccgacttcg aaatcaccga
      841 agcgatgctc cgccatttca tccagaaggt ccatcaccgc aagctgatcg ctccgcgggt
      901 tgtcgtggcg gtcccatccg ggatcaccga agtggaaaaa cgggcggtga aagattcggc
      961 gacgcacgcc ggcgcacgtg aagtttatct catcgaacaa ccgatggctt cggccatcgg
     1021 ggtgggactg ccggtgcatg aaccggcagg caacatgatc gtcgatattg gcgggggaac
     1081 ttgcgagatc gcaatcattt ccctcgcagg catcgtcttc agccgcagcc tgagggtcgg
     1141 cggagacgaa tttgacgaga cgatcgttgc gcacatgaag cgtgcttata acttgatgat
     1201 aggcgaacgc accgcggagg agataaagat ccggatcgga tcggcctttc cgctggagca
     1261 ggaattgacc atggaagtca aaggccgcga cttgagcgcg gggttgccga aaaccctgac
     1321 cattcgctct gaagagattc gcgaggcttt gcaggagccg ctttcaagta ttttggagtc
     1381 tgtgcgcatc acgctggaac gttgtccgcc tgagctttcc tccgatttgg tggatcgcgg
     1441 gctggtgatg gcaggtggtg gcgctttatt gcggggtatt gatcgtcttg ttgctgagga
     1501 aaccgggttg ccggtgcata tagcagacga tcccctcagc gccgttgccg aaggcacggg
     1561 gcgcgttttg caggaactcc aattcttgaa gcaggttacg tccaattcca aggtttgatg
     1621 ccgggtgcgc tcgcccgcat agcgggttga gcatgttaaa aagaccgcat tacatggcct
     1681 tagggctggt cggattgctg acattgattt tggtgaattt gccgcatcac acggcgaatc
     1741 agatcaaact tgccatcggc agcctttatc tcccgctctt cggactttcc aaatcttccc
     1801 agcaagtgct gaaccaggca agcgacgcaa tgacgccgcg ttccacattg ctgctgcaaa
     1861 atgaggaatt gcgccgcaca aatcaggaat tgcaggtccg cgccatgcag gcggatgcct
     1921 tgcaacgtga gaacgaccgc ttgagacaac tctttggctg ggagaagcaa acgacgtggc
     1981 agttgcgcct tgcgcgagtt gtggcgcgtg atccggccaa ctggtggcat acagtgacaa
     2041 ttgatcttgg ctcgcgcgac gggatgcgtc ctgatttgcc ggtgctgacg ccatcgggtt
     2101 tggtcggacg aatttcacgg gtcacgttga ccagctccga agtggtcctt gtcggcagtt
     2161 ccgaatgcaa ggtggctgcg ttggtgaagg aaaccggcga taccggcgtc attaccggcg
     2221 gcgcaagtcc tttgggcaac agcagtcttg cgactttaag ttacctggcc agcagcgcca
     2281 atctaaagcc cggccaaaag gtgcagacct ggggcgaagg caacgtgttt ccaaagggca
     2341 ttgtgatcgg ccaggtggcg gaggattcac ggcaaactga attgggttat actgaagcgc
     2401 gtgtgaaact tgcggccgac tttggcgcat tggaggaagt gtgggtgctg atgcgatgaa
     2461 ctggctgact ccaattttaa tcctgcttgt ggcattcgtg accgtttatc tggaggccac
     2521 atttactgga gttcggcact tcctgggcgc acagattgat ctgctgcccg ccttgatggt
     2581 ttatgcaagc ctcagtgcca gtttggcgac catcagcgtc cttgcagttt gtggcgggct
     2641 ttggttcgac tcgctttcct caaatcctct tggcatcagc atccttccat tgttcctttc
     2701 gggctacttg atttacctgc gacgtggatt gattttaagg gaacaacact ttgcccagct
     2761 tgtactcgga ttgctggcca gttccatcgt gccgctgctg acattgcttt tattattgag
     2821 ttcgcgtcaa ccgctcgtcg tggggtgggg gacgctttgg caatggattg tgatgagcct
     2881 gggtggatgc gttttgacac cggttatctt tcgtttgttc gacggattgc accacgcgct
     2941 gaggtatcaa ccggtggtgg aaaccagttt ccggctggac cgcgaaatca gaaggggacg
     3001 caagtaaaac aatgctggta ttcgaccaac ttaaaaaaga cgacccgcaa ctgcgatttc
     3061 tcgcggtgat ggtgctgtgc gggatgttgg tcttgcttgg cggcctttgg tgggtgcagg
     3121 tggtttcctc gcgcgtttac cggcagagcc ttgaaaggca atccgtccgg acgattcgca
     3181 ttccgcccat ccgcggcagg attttggacc gtgatgggcg accgctcgcc gagaaccgtc
     3241 cgagttacaa tgtggatttg tatcttgaag aattgagcaa gaatttccag aaaggatatt
     3301 cggccgccat caaggatgca accaactggc ttcatcttca actggtggaa aaggagaagc
     3361 agttgggccg aaaactgacg ccgcaggaaa agaagcaatt tgccttgagc cgtgcattga
     3421 gagacggatt gcagcaacaa acacgctatc aggtcatcag caatctgatt gataatctca
     3481 gcgcgcggtt gcaggagccg gtcaacctca cgccaaaaga atttgaaacc cattatctca
     3541 aagcgcgggc actgccaatg tccatcctgc caaacttgaa cccggtccag gtcgcgcggt
     3601 ttgaggagca atctgctgcc gagcctggca tggatttgga agtgcattcg atgcgttatt
     3661 accccaacgg aaccattggc gcgcacctgc ttggatattt ggtacaccat aatgagtccg
     3721 acgacaacga cgacgcggaa cacaaataca attaccggct gccggaattc gcggggagtt
     3781 cggttgggat tgaaggcgtt tttgacaagg aactccacgg cattgccgga gaaaaatcgg
     3841 tggtcgtcaa ttatcttgga tacaagcaat cggagaacat ctgggcgcct cccgaagtcg
     3901 gccaggacgt ggtgttgacg attgatttgg acattcaaaa ggccgctgac gccgccttgg
     3961 attcagtcaa agaagatgtt catggagcag ttgtcgtcat ggatgttcgc aacggtgatg
     4021 tgctggcgat cgcttcgtcc ccgacttaca atccaaacca tttcatccag cgtccgccgc
     4081 aggaaacttg gtcgcgcgaa tgggagcgct ggaccaacga agatttgcaa gtgcagatga
     4141 accatgcgat gcaagggatg tacgcgcccg gttcaatttt caaaattgtg gtgggactcg
     4201 cagcattgga acagggtgtg ttggatccgc acgagacgtt tcatagcctt ggttattatc
     4261 cgattcccgg gagcaagccg attggcgaca cggccggcgc gggcgagttt gattttgaca
     4321 gggccttggc caaatcgtcg aacccttatt tcatcaccca aggagtcaag ccaggcgttc
     4381 tggcgaagat ggtcgccttg gggcaacggc tgcattttgg tgaaaggaca ggactgctcc
     4441 cgcatcagga agatcgcggc tatttcccca cacccaagga cattgcatcc cgttcgtggg
     4501 ttccaggcta tacaatgcat ttaagcatcg gtcaggacag gattgcagtc acaccggtgc
     4561 agattgcggt gatgatttca gcgattgcaa acggcggaac ggtgtattgg ccgagaatcg
     4621 tttctcgtgt cgaaagcgcc ggagatttgc cggcccaaac ttttcccggc ggacgtgtgc
     4681 gcgatacgtt gggcatcagc gcgcgcagcc tgcgcatcgt gcacgaggcc atgtctgcag
     4741 atgtcgaaag tcctgaaggc accggccatg cggctgctgt tcctggacta cgcattgcgg
     4801 gcaagacagg gactgcggaa gtggagaaaa atggtcacat tgacaaagct tcaaaaatca
     4861 catggttcgc ttcttttgcc cctgtcgaaa atccgcatta tgcggtgatc gccatggtgg
     4921 ttcacggtgc gtcaggcggc cttacctgtg ccccgttggc ccataaagtt tatgaggcca
     4981 tccaggaaag cgaacgaaag cctcacgcga aaacgggaat actggccgag acacgataaa
     5041 aatgttcaac gcgatacaaa atgaacgtcc gtcgagggtt gattacattc aactcgcggc
     5101 ggtgctttgc ctgatgctca tcagcgtcgc gtttgtattc agcgcgacga cggtgaacga
     5161 ttcgggcagc acactcgctt ggtatcgaca aatttatttc aagcaagtca tctggtttgc
     5221 ggtgggactg ggcgcagcag gagttgtttg cctgatggat tatcacagtc ttacgcgttg
     5281 ggcgctggtg gcgtatgcgg cctccatctt tttcctgatc gtggtgctcg tccccgggat
     5341 tgggacgatg gtttatggtg cgcgccggtg gattggtttg gccggctttc aatttcagcc
     5401 gtccgaattc gccaagctcg cgctcattct tgcgttggcg cattttctca gccgtccggt
     5461 ggatgaactc cggcatcggc acattttctg gaaatccatg ggtttgatgg tgcttccgtt
     5521 tctcctgatc atgaaagaac cggacttggg ttcggcgttg gtattgatcc cgacagggtt
     5581 cgcaatgttg tatgcggcgg gcacgccgaa acggtatctt atccaattga tcatgttcgg
     5641 agggatcatc gggacggtat ttttgggcga tattttattc gcgccgccaa aataccgcat
     5701 catcaagttg caggattacc agatgaaacg tctgctggtc tattttggac gcaattacac
     5761 cgcatatgca ggacccggcg tcacggaggc ggagaaacaa cgcctgcggg aacgggaact
     5821 gaatgaccgt tataatgtcg agcaggcttt gattgcagtc ggttcgggcg ggttgttggg
     5881 caagggctgg cgacagggcc agcaaaatgc cctcgggttt ctcccgcaag caggcaagca
     5941 caatgacttc atcttttccg tgatcgcgga ggagaaggga ttcatgggca gcgtgacagt
     6001 gctcacgctt tattccgtga ttctgttcac agggatcaga atcgcgggac aggcgcgcga
     6061 tcgcctcggt aaattgctgg cggtgggcgt ggtcacgctc atcttcagcc atgtcttcat
     6121 caacatcgga atgaatatcc gcatcatgcc tgtgacgggc gtgccactgc cgctactaag
     6181 ttatggcgga tcgtcagtgc tcggctcgtt gatcgccatg ggcatcttgc aaaacgttta
     6241 tatgtatcga aaggcttatt agagtatgag tggaaataat ttttcgcggc gacgccgggg
     6301 cggcatgcgc ttccggccca gcagcgggct caatccaaat cccaaccgtc cggaccgcga
     6361 ggcgcttgag gctcgcgccg aggtggtcgg cgaaaaggcg ccgcaggaca agctgtatga
     6421 ccgccgacac gcgcaggaga ttgagcgcgc ggagaacatt gccgccggtt tgccgcccga
     6481 gggcgcgcca aaaccggctg agagtgttcc catcgaaggc gaccgcaaag acgcttttcg
     6541 cgaaccgaat ctcgaaactc ccgccgaagt gcgggaagaa aaaccctttg cgccagttcc
     6601 tgtaaaggaa cgtccccaag gcatcgttga aaccatccgc gccgcggcga cccagatgat
     6661 caagaaggtc catcggctca tcaagccggt gaagcggacg cacaaggaag tcatcatcaa
     6721 cgcggagtcg ctcgaaacgc gcgtcgccgt catggaagaa ggcaagctcg aggaattcac
     6781 catcgaacgc accacggagg agcgtttggt gggcagcatt ttcaaaggca agttgcgcaa
     6841 cttgcaggac gatctaaagg cggcgttcgt tgacatcggg tttgagaaga acgcgtttct
     6901 tcattactgg gacattgtcc cgagcaactt cgacagcggc gtggaagtgg tcgagcgaga
     6961 aaccaagcgc cgcgaaaaac cgcgcatcac gcaaaaggac attccccgtc tttatccgcc
     7021 tggcagcgac atcatcgtgc aggtgacgaa gggtcccatc ggcagcaaag gtccgcgcgt
     7081 cacaaccaat ctggtcttgc ccggacgtta tctcgtgctg ttgccgaact ccgatcaaag
     7141 cggtatctcg cgcaagatcg aaaatcaaca ggaacgccag cgtctcaaaa aaatccttcg
     7201 cgaactcacc attcccgaag gcatgggcat catcatgcgt accgtcggcg aaggccagca
     7261 ggcgcgctat tttgtgcgcg acctcgcgct cctgcttgaa gaatggaaac tcatccagga
     7321 acgcatcaag agccaggctt ccgcgacctg cgtcttccag gaaccggacc ttatcgaacg
     7381 caccgttcgt gatttcttga cggaagatgt cgaacgcatt gttgtggata acggcaaggc
     7441 gtacgaacgg atgcgggaaa tgatctcgaa gatttcctgg cgcgcggctg agaaggtcaa
     7501 attgtacaac gaatcgcagg tgatcttcga tcgtttcaac gtcagccggc agttggaaaa
     7561 cgccttctct cgcaccgttc atttgaagag cggcggttac atcgtcgttg atgaaaccga
     7621 ggcgttggtt gccatcgacg tcaacactgg gcgtcacaaa ggcggcaagg accaggaaca
     7681 ggcgatcctc aaagtcaacc tcgaggccgc tgaggagatt gcgcgccagc ttcgcctgcg
     7741 caacatgggc ggtttgatcg tgctcgattt catcgacatg aaacatccac gcgaccgtca
     7801 acaggtttat caacggatga aggacggatt gcgccgcgac aaggccaaga cacacatcct
     7861 gccaatctcg caacttggcc tgatggagat gacgcgccag cggcattcgg aaagcgttca
     7921 ttccgccgtc tatgatgaat gcccgtactg caaagggcgc ggaaaggtta agagcgccct
     7981 gacgatgagc gtggaaatcc aacgcaagct cggcgaaatc ctcaagaaac gttcgcgcga
     8041 cgaatcggat ttccaattgc gaatcgtcgt gcatccaagc gtgctggacc gtttgcgcaa
     8101 cgaagacgag aagcatctca tcgagatgga aaaacggtac ttcggcaagc tgtcgttccg
     8161 cgccgacccc gcgctgcatg ctgaaacatt caagatcatc aacgtcgcaa cgaatgaaga
     8221 aatgagctga tcgaaattag aattgaactt gttcgaaaac gccctgtaga aatgcggggc
     8281 gtttttttct aaaatttatt tcgacgctgt tggggcaggg gcggctttgc ggatggtcac
     8341 gagccacgcg gtttcattcg gcgcttctcc gccgagaacc agcagtccat tgctcttgag
     8401 cgattccttg tgcatggaaa caggcttgcc attgccatgc aatctcactt cgacgcagtc
     8461 gcctccgaga tttttgatgt ccagcgtgca acggtcgctc atgacaatct ttgatttcgt
     8521 ctggccgacc ggaatttcaa ccactcgttg gcattcctca aaataatttt tccagcgata
     8581 gggcgaacct ttcaacctct tggtcagcac cgggtccaac agatgatgct tggggtttgg
     8641 agaatgctgg tcatcagtgc cccaaaccag tttggcctcg accctcaagt tctgcgcgcg
     8701 cgcggaagtt gcgagcaaca acgccgcgca aatgcaaaac aacggtttgc aaaacttaaa
     8761 tccccgcgtg aacaaaaaca tcttcattcg ggtgtgacat tatcaatatc ggaagaatct
     8821 gcaacggtgg aatccgtgtt attgcggtcg tagagccaga ccatggtcat cttgttggct
     8881 tgatcgcggt aggtataaga gcccatgtca tcggaagcca cttcgatttc accgagttga
     8941 tttccggggc cgcgagaatg gaccgccaca aaccccagca aaacgcaaac caaggcgaag
     9001 gtgccaactg gcaggaaatg gcgttggatc cacgcgaacc atgattgagc ggcaaccggg
     9061 cgcgccggtt gttcgctctc gcgctcgatt tcgcgctcga tcttggacca gaaaaattcg
     9121 cggctctccg gcaactggca ttccggctca ttgccggcga gcgccgcctc ggtgtttcgc
     9181 aattcggtca acagggcttg ggcttcggca tcccgggcca gcaaatcccg cgcctcggcg
     9241 agttcatggc cggacaattc gccgtccagg aacgcctgca acttcaattg tgaatcgtga
     9301 ttcattttaa tcttccgttt tcaaacttgc cagcaacacc gccatctttc gccgcgcgta
     9361 aaacaaccgg gacatcaccg tcccgatcga gcaattcatc gtccgcgcga tttccttata
     9421 ttccatctct tcaaattcat gcagcaccag caccgtccga tgctcatgcg acaattgacc
     9481 cagggcaatg tcaatccgct tgcgcaactc accgcgttcc agccgctcgg tcgggttcac
     9541 gttgaccacc ggcatctgcc gttcaacgcc gcccgattcc tcggacattt cttcgatgga
     9601 ctcggtgcgc tgcctcttgc ggcgccgcaa ctgatccagg caaagattga tgacaatccg
     9661 cgtcatccaa gtcccaaaac tcgattcgcc ctggaactgt ttcaacctct gccagctctt
     9721 cacccatgct tcctgagaaa gatcaagcgc ctcgtcctcg ttccgcatca tgctgaacgc
     9781 gcgggcataa attttatcgc gatgcctcgc caccagctct tcaaaagcgc ccatgtcgcc
     9841 ccgctttgcc gctccgacca gttgctcatc accggcggaa gcgtggtctg gctttgactt
     9901 catgggttgg acgggctgca ttaaatcgct attcaacgga tttacaactt tcctttggtg
     9961 ctgggcactt gtccagcgat ccgcgcatcc tgttggcacg caatatctac cgctttggca
    10021 aatgccttga acaacgcctc gacgatgtga tggggttcat ctccgtaaag cagctccaaa
    10081 tgcaggttgc aacgcgcttc gttggcgaag ccctgaaaga attcccgggc caaaccgaac
    10141 cggaaagcgc tcgacatgtc ctgagttttt tccttcgcgc tgagcttgcg ataggccatc
    10201 tcattcatcc cgcgccagac gagaaaggga cgcccgctga aatcaatcac gcaccgtgcg
    10261 aggca
//
LOCUS       DASZMM010000503         4874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4348194, whole genome shotgun sequence.
ACCESSION   DASZMM010000503 DASZMM010000000
VERSION     DASZMM010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4348194"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VG754_12085"
     CDS             complement(<1..122)
                     /locus_tag="VG754_12085"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWF20003.1"
                     /translation="MNYFKAIAAMSENRVIGHAGKIPWHLPDDFKWFKKMTTGQV"
     gene            complement(119..418)
                     /locus_tag="VG754_12090"
     CDS             complement(119..418)
                     /locus_tag="VG754_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF20004.1"
                     /translation="MDYKIVWSDRSLSNLKEIVQFIAEDNALAAERMGNTIIKRASFL
                     AQFPQLGAVFSGLRRDDVREIAAPPYRIIYQVLESKKMISILTVWHGARQEPEIQ"
     gene            complement(382..588)
                     /locus_tag="VG754_12095"
     CDS             complement(382..588)
                     /locus_tag="VG754_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20005.1"
                     /translation="MTDQELILDAVRKMPKTASLAEILDELRLLQSVAQGLAETERGQ
                     VTPHDEVAKKLGAWITKSSGRTAA"
     gene            complement(670..1059)
                     /locus_tag="VG754_12100"
     CDS             complement(670..1059)
                     /locus_tag="VG754_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20006.1"
                     /translation="MVKKLLPVLMLGLAMAGCTTTTSVTNLTPKREFRNENGFYPIEA
                     AFHSSQQSLRWETVKPEVIIEKDVYPMRFTPLMTNRWETLIPVPPGNNVVYYRFKFDY
                     KYNAFGAPPQPDSTLSPIYRLQIIDKQ"
     gene            1377..3083
                     /locus_tag="VG754_12105"
     CDS             1377..3083
                     /locus_tag="VG754_12105"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HWF20007.1"
                     /translation="MIGTIRRHQTWLWVVIITLTVVSFVIFGPTNTKIGNALSGGGNA
                     NLGYVGGKPITQNEFNDARKEVALGYFLQNQKWPREDASPEINREIYQRLFLNQKEKE
                     LGIQISTEAIAGVAKRMLDEIARGSHLNQLSLDDFTDKVLKQGGLDANDFDRFLRHEL
                     AMEQLTKIAGLSGNLVTPQEAETLYRLEHQDIQTSMVLFSASNYLASVAVTPEEISKF
                     YTNQMANYRIPEQVQVDYVRFSLTNYTAAAMTNLTNLDQLVDNAVTQMGTNLFHGAKT
                     PAESKLAVRTEIVRRTSVKEAYNAAHKFANDLETMQPRNADSLRALAASNHMTVRTTA
                     PFDRDYGPSPADVIVAPDFTRKAFQLTTDDPFAGPILPDESHPSDVFVMALKDHTISR
                     VPPFKEIEGKATSDYRYALATQQAQQAAVRFDNLVTNGLARGKSFSAICADSGIKPEP
                     LPPFNLNTRSLPDAIEQNINFNILRQVAFSTPPGKVGPAAPARGGAFVLYVDKALPID
                     EAKLKEEFPSFLAYVRQARQSDAFNQWFSGEVRRDPEFMQTLQQLTEQAQKSGSSRRP
                     SS"
     gene            3114..3917
                     /locus_tag="VG754_12110"
     CDS             3114..3917
                     /locus_tag="VG754_12110"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWF20008.1"
                     /translation="MSEVIKLSSPATREFWELPILFEDEHLLAVDKPSRMLTSPDRYD
                     PKRPNLMKLLHGGIEQAKPWAKQRALNYLMNAHRLDFETSGVILLAKTKPVLVKLASM
                     FGSETLVQKTYVALVHGNPVKDAFEVDAKLAPHPVKIGLIRVDQKNGKHAVTKFELLE
                     KFSGYSLLQCHPLTGRTHQIRVHLRQAGLPITGDALYDDSGPLLLSRLKKDYHLKPGR
                     IERPLMDRVALHATELKLPHPVTGAEIVIASPWPKDLTVALKYLRQYAI"
     gene            4213..>4874
                     /locus_tag="VG754_12115"
     CDS             4213..>4874
                     /locus_tag="VG754_12115"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWF20009.1"
                     /translation="MDMNLVAPLKILYQIESARIEDCRKLCKVLSLRRMPVIEDILIQ
                     NEQNIGLLDISFLEQIGKTINDRDIQRNITDWPFENFWGNPYEPSHSKLLKFFIDPNE
                     EHDCGCYLLKKLLEILIDALPGEQNFQVDEHCKVSQPNYIDLLIERHGDNGKYAIIIE
                     NKINWAPNQEKQLQTYVEAVMRRDLKPKQIYVFYLPLTSEKHPNSDDQDTLTKKFKVN
                     YR"
BASE COUNT         1218 a         1254 c         1260 g         1142 t
ORIGIN      
        1 acctggccgg tggtcatttt tttgaaccat ttgaaatcat cgggcagatg ccagggaatt
       61 tttccggcgt ggccgatcac gcgattttcg gacatcgccg cgatggcttt gaaatagttc
      121 attgaatttc aggttcctgc cttgcgccgt gccaaacggt caaaatgctg atcatttttt
      181 tagattccaa gacctgatag atgatgcgat acggcggcgc ggcaatctca cgaacatcgt
      241 cccgacgaag ccccgaaaaa actgctccaa gctgcggaaa ttgcgccaaa aacgaagccc
      301 ttttgatgat tgtgttgccc attctttccg ccgcaagggc attgtcctca gcgatgaatt
      361 gaacaatctc cttgagattg ctcaggctgc ggtccgacca gacgattttg taatccatgc
      421 ccccagtttt ttggcgactt catcatgcgg agtgacttgg ccgcgttcag tctcagccag
      481 cccttgcgcg acggattgca gcagcctcaa ttcatccaga atctcggcga gcgaagccgt
      541 ttttggcatt ttacgcacag cgtccaaaat gagctcctgg tcggtcataa caaattcacc
      601 atggccgcga acaagccaga aatcaaatca tatcaatccc tggacccggc gctccgaagc
      661 gtcaagccct cactgtttgt caataatctg cagccggtaa atgggcgaaa gcgtgctgtc
      721 gggttggggc ggcgcgccga aagcgttgta tttgtaatcg aatttgaacc ggtaatagac
      781 gacgttgttg ccgggcggca cggggatgag ggtttcccag cggttggtca tcaacggcgt
      841 gaagcgcatc ggatagacat ccttctcgat gatgacttcc ggcttgacgg tttcccagcg
      901 aagcgattgc tggctggaat ggaacgcggc ctcgatggga taaaatccgt tttcgttgcg
      961 gaattcccgc ttcggagtga gattggtgac ggatgtggtg gtggtgcaac cggccatcgc
     1021 caggccgagc atcaaaactg gcagtagttt cttaaccata aatattgcag cagggttaca
     1081 taagcggcgg ggggttgtaa agattggatt tcgcctcatt tttcagcaga aaatgcataa
     1141 aatcgaacgc ccaacatcga acgttgaacg ccgaatgaac ccgattcagc gttgggcgtt
     1201 caatgttgga tgttcgatgt tttccaatca gtgtttaggc caaggctttc aactgcgcag
     1261 cgatctgctg cttgtcgcac ggtaaaaaac agccaaaaaa ttcctttttt gcgtttgacc
     1321 atccttgccg tgcgcggtta ggttaaccgc tcttaatccg gattaattta tcgaatatga
     1381 ttgggactat cagacgtcat cagacttggt tatgggtggt catcatcacc ttgaccgtcg
     1441 tcagctttgt catttttgga ccgacgaaca cgaagatcgg caatgcgctt tcaggaggcg
     1501 gcaacgcaaa cctcggctat gtgggcggca agccgatcac gcaaaacgaa ttcaatgacg
     1561 cccgcaagga agttgctctt ggctattttc tccaaaacca aaaatggccg cgggaggatg
     1621 cttccccgga aatcaaccgc gaaatctatc agcggctctt tttgaaccag aaggaaaagg
     1681 agttggggat tcaaatcagc actgaggcga ttgctggcgt cgccaagcgc atgcttgatg
     1741 agattgcgcg tggatctcac cttaatcaat tatccttgga tgattttact gacaaggtgc
     1801 tgaaacaggg cggcctggat gcaaacgatt tcgaccgatt cctccggcac gaactggcga
     1861 tggaacagtt gacgaagatt gccggcttga gcggaaatct tgtgacgccg caggaggctg
     1921 aaacgcttta tcgtttggaa catcaggaca tccagacttc gatggtgtta ttttcggcgt
     1981 ccaattatct tgccagcgtc gccgtgacac cggaagaaat ctcgaaattt tacacgaatc
     2041 agatggccaa ttaccgcatc cccgaacaag tgcaggtgga ttatgtgaga ttcagcctca
     2101 cgaattatac cgccgctgcg atgaccaacc tgaccaactt ggatcaactc gtggacaatg
     2161 cggtgacgca gatgggaacc aatcttttcc atggcgcaaa aacaccggcg gaatcgaagc
     2221 tggctgtgcg aaccgaaatc gtccgccgca cttccgtgaa ggaagcgtat aacgcggcgc
     2281 acaagttcgc caatgatttg gagacgatgc aaccgcgcaa tgcggacagc ctccgcgcgc
     2341 tcgcggccag caaccacatg accgttcgca ccacggcgcc attcgaccgc gattacggtc
     2401 cttcgcctgc tgatgtgatt gtcgcgccgg attttacccg caaagctttt caactcacga
     2461 ccgacgatcc gtttgccggg cccatccttc cggatgagag ccatccttcg gatgtttttg
     2521 tcatggcgct gaaggaccac accatttcgc gggtgccacc gttcaaggaa atcgaaggca
     2581 aggcaacgtc ggattaccgt tacgcgcttg cgacgcaaca ggcgcaacag gccgcggtcc
     2641 gtttcgacaa cctcgtgacg aacggtcttg cgcgcggcaa gtcgttcagc gccatctgcg
     2701 cggattccgg catcaaaccc gaaccattgc ctccgttcaa tctcaacacc cgcagcctgc
     2761 ctgatgcgat tgagcagaac atcaatttca acatccttcg tcaggttgct ttcagcacac
     2821 cgccgggcaa ggttggtccc gccgctcccg cgcgcggcgg cgcgttcgtg ttgtatgtgg
     2881 acaaggcgtt gccgatagat gaagcgaagc tgaaggagga atttccctcg ttcctggctt
     2941 atgtgcgtca agcgcgccag tctgacgcct tcaaccaatg gttcagcgga gaagtgcgcc
     3001 gcgatcctga atttatgcag accttgcagc aattaaccga acaggcgcag aaatccggca
     3061 gttcacgccg gcccagttcc tgattgcgcc cgacgttcgc cgggaattta tccatgagcg
     3121 aggtcatcaa actttcgtca ccggccacgc gcgaattttg ggagctgccc attttgttcg
     3181 aggacgaaca tctgcttgca gtggacaagc ccagccggat gctcacctcg ccggaccgtt
     3241 acgatcccaa acgaccgaat ctgatgaagc tgctccacgg cggcatcgag caggcgaagc
     3301 cttgggcaaa acagcgcgcc ctgaattact tgatgaacgc gcaccggctc gattttgaaa
     3361 ccagcggcgt cattctgctc gccaagacga agccggtgct cgtgaagctt gccagcatgt
     3421 ttggctcgga aacactcgtc cagaaaacct acgtcgcgct ggtgcacggc aatcccgtga
     3481 aagatgcctt cgaagtggat gccaaacttg cgccgcaccc ggtcaaaatt ggtttgatac
     3541 gagtggacca aaagaatggc aaacacgctg tgacgaaatt tgaattgctg gaaaaattct
     3601 ccggctattc attgctgcaa tgccatcctt tgaccggccg gacgcaccag attcgcgtcc
     3661 atttgcgcca ggctggattg ccgatcacgg gcgatgcact ttacgacgac agtggtccct
     3721 tgttgctgtc gcgtctgaag aaagattatc accttaaacc gggacgcatt gaacgtccat
     3781 tgatggatcg cgtggcgctc cacgcaaccg agttgaaact gcctcatcct gtcaccggcg
     3841 cggaaattgt catcgcatca ccatggccga aagacctgac agtcgcgttg aaatatttga
     3901 ggcagtacgc gatttgaaag tttatgttta ggattttggc ttgagcttgc catcgcggat
     3961 acaatgcaat agttttgccc ttgtgagggc gcaatcgtac aaagaactga aaaggcctga
     4021 ggcgcgcggc gcaagtcagc tatgcgccag ccacgcaaaa gtgcaagatc agcatccgcg
     4081 tcgcaaaggc gcgcttcatt cgttgggaat gggcgtcgtt ggcgctttac taatttcaca
     4141 caagtggcgc gggcgatggt aaaatggttg taaggaactg aattctaagg agccgtatta
     4201 atgcattttt gaatggatat gaatctcgta gcgccgttga aaatcctata tcagatcgaa
     4261 tctgcgcgga ttgaagactg ccggaagcta tgtaaagttt tgtcacttcg taggatgccg
     4321 gtgatcgaag acattcttat tcagaatgaa caaaatatcg gattgttgga tatttccttt
     4381 ctggagcaaa ttggaaaaac aattaatgat cgcgatatac aacgaaatat aaccgactgg
     4441 cccttcgaaa acttctgggg aaatccatac gagccgagtc attccaagct gcttaaattt
     4501 tttatcgacc caaacgagga gcatgactgc ggatgttatt tgctcaaaaa acttttggag
     4561 attctaatag acgcccttcc tggtgaacaa aattttcaag ttgatgagca ttgcaaagtc
     4621 tcgcaaccaa actacatcga tctcttaatt gaacgacacg gggataatgg aaagtacgcc
     4681 atcattatcg aaaacaaaat aaactgggct cccaaccagg agaagcaact tcaaacgtat
     4741 gtcgaagctg taatgcgacg tgatcttaag cccaaacaaa tatacgtttt ttaccttccg
     4801 ttgacgtctg agaagcatcc aaattcagac gatcaggata cgctcacgaa aaaattcaaa
     4861 gtgaattatc gcca
//
LOCUS       DASZMM010000504         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1348752, whole genome shotgun sequence.
ACCESSION   DASZMM010000504 DASZMM010000000
VERSION     DASZMM010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348752"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1115)
                     /locus_tag="VG754_12120"
     CDS             complement(153..1115)
                     /locus_tag="VG754_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HWF20010.1"
                     /translation="MKAIRVHQFGGPEVLRLEEVPKPQPGPGQILLRNHAVGVNPVDT
                     YVRSGAYPAKPNLSYTPGADAAGLIESVGVGVNSFKPGDHVYVGGSLTGTYAEYALCE
                     PAQVHPLPEKITFQQGAGVNTPYATAYRGLFQRAKGVAGEVVLVHGATGGVGIASVQL
                     ARAAGLAVIGTGGTENGRRLVAEQGAHHVFDHRAPDYLKQILELTGGQGVDLILEMAS
                     HVNLGKDLTILAKNGRVAVIGSRGPVEINPRDAMSREAAILGVVLNNASPKDLAGIHA
                     ALVAGLENGTLRPVVGKEFPLAEAAAAHKAVLEPGAFGKIVLLP"
     gene            complement(1298..>1796)
                     /locus_tag="VG754_12125"
     CDS             complement(1298..>1796)
                     /locus_tag="VG754_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF20011.1"
                     /translation="AHAVHPITALQSEYSLWSRDPEQEILPTCRDLGIGFVPYSPLGR
                     GFLTGRFKKPEDLSPEDYRSKTPRFQGENFQRNLELLRRIEQMASEKHCTPAQLALAW
                     VLAQGEDIVPIPGTKRRKYLEENVGALNVQLTPAELARINEVAPQGAAAGTRYPEAMM
                     HTVNR"
BASE COUNT          393 a          496 c          526 g          381 t
ORIGIN      
        1 gtaaagaatc gggatgatga tgtcgccctc gcttaactcg atctgtttgg gtttcgccac
       61 gttgaaaaga tggttacaag gatacaaagt tacaaggttg caaaaaagga ctttgccgcg
      121 cgcaagcggg aatgccagcg gattggaggc ttctacggca atagcacaat ttttccaaac
      181 gcaccgggtt ccaacacggc cttgtgcgcc gcggctgctt cggcaagcgg gaattccttg
      241 ccaaccaccg gtcgcaacgt gccgttctcc aaacctgcga ccaacgccgc gtgaatcccc
      301 gccaaatctt ttggcgatgc gttgttgagc accacgccaa gaattgcggc ttcgcggctc
      361 atggcatcgc gcggattgat ctcgacgggg ccgcggctgc cgatgaccgc aacgcggcca
      421 ttttttgcaa ggatggtcag gtctttgccg agattcacat gcgaagccat ttcgagaata
      481 aggtccacgc cctggccgcc ggtcagttcc aaaatttgct tgagataatc gggcgcgcga
      541 tgatcgaaca catggtgcgc gccttgttcg gccacgaggc ggcgtccgtt ttctgtgccg
      601 ccggtgccga tgacagccaa tccggccgcg cgcgccagtt gcaccgaggc gatgcccacg
      661 ccgccggtcg cgccatggac caaaacaact tcgccagcca cgcctttggc gcgttgaaac
      721 aatccgcgat atgccgtggc gtaaggagtg ttcacacccg cgccttgttg gaaagtgatt
      781 ttctctggca acggatggac ttgcgccggt tcgcaaaggg cgtattcggc gtaagttccg
      841 gtgaggctcc caccgacgta aacatggtcg cccggcttga acgagttcac gccaacgccc
      901 actgattcaa ttaagcccgc agcatctgca ccgggcgtgt aagacagatt tggcttcgcc
      961 gggtaagcgc cggagcggac gtaagtgtcc accgggttca cgcccacggc gtggttgcgc
     1021 agaagaatct gtccgggacc gggctgcggc ttgggaactt cttcaagccg caaaacttcc
     1081 ggcccgccga attgatggac gcgaatggct ttcacaatgc aaataagttg ttaatgttta
     1141 tgttctgaat catgaaaaaa acgctcttca cgcaattcaa gcagagtccg cgagaagctg
     1201 ctcgtattag acgcggcgaa atcaaacctt caaggatttt caaagttaag tctgaaaaaa
     1261 tcagggcctc ctcacgtcgg cagccaccca aataaggtta ccgattgacc gtatgcatca
     1321 tggcttcggg atagcgcgtg ccggcggcag cgccttgagg cgcgacttca ttgatgcggg
     1381 cgagttcagc gggagtcagt tgaacgttga gcgcgccgac gttttcctca agatatttcc
     1441 ggcgcttggt gccggggatg ggaacgatgt cttcgccttg cgcaagcacc catgcaaggg
     1501 cgagctgcgc gggcgtgcaa tgtttttccg atgccatttg ttcaatacga cggagcagtt
     1561 ccaaattgcg ctgaaaattt tcgccctgga aacgcggcgt cttcgaccga taatcttccg
     1621 gcgacaaatc ttcgggcttc ttaaaccggc cggtgagaaa accgcgtccc aatggactgt
     1681 aaggaacgaa gccgatgcca aggtcgcggc aggtcggcag gatttcctgc tcgggatcgc
     1741 gtgaccagag ggaatattcg ctttgcaacg cggtgatggg atggacagcg tgggcg
//
LOCUS       DASZMM010000505         3086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2691099, whole genome shotgun sequence.
ACCESSION   DASZMM010000505 DASZMM010000000
VERSION     DASZMM010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691099"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1773)
                     /locus_tag="VG754_12130"
     CDS             complement(55..1773)
                     /locus_tag="VG754_12130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWF20012.1"
                     /translation="MNDQDLKDLWQKQTEPAAPSLSADDLTAKMRGKMSKWRRQIIAR
                     DARELAACAFIIIGFGYYFFSIPTLVTRIGDIILIAAAVFIGAKLVRTRNANPPAQPS
                     ASVIESLHAERRSVHSQTELLRTVVWWYLLPLFIGTLVFAWGTPGRELSWKIDFSIGV
                     LALHVVLYFCNQYALRKKLRPMIAQLDALIQGMETGQFDEIIMQTQTIASITAEPAAP
                     SRVKIVLINIAESTLALFLCIAGLFAYGGGIFTNFVYMLLMLLVPLSIVVFVFKAALG
                     KIYYGLLFNPFAKQSRTYWISALVRLAFFASCGYLLSIAIAPCQSPQHARHYMQTVTW
                     SLAALQIVLALMPARRISIPFIAMYSIGACFMIWHFVQIAAPLKTEKVLLDSPLKGVA
                     CVLQGGNDVIINHHYLLHSQRDALDIFKVADTVQQIKDWKIMKGDAGFGETIYSPCSG
                     HVSHVESNHRDNKKGQTDKVNVAGNYITIEIAPDRYVLIAHLKRNSPTVKVGDVVKAG
                     QPIAQCGNSGNTSGPHVHIQVQASPDFNYDTKTFPIAFHHVLRGDKELENFQAKRNDL
                     LLNTEN"
     gene            complement(1766..2290)
                     /locus_tag="VG754_12135"
     CDS             complement(1766..2290)
                     /locus_tag="VG754_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWF20013.1"
                     /translation="MKPTQPDDQQFLELVNENRNRILKVCRVYAWNAADQDDLYQEIV
                     FQIWRGLPALKEKQFANTWLYRVAINTAISFVRKRASRGDRVLHVDHTQLARTIEAQQ
                     TVEPQPDDRLANLYAAIAKLNELEKALVTLFLEDFTYDQIAEVLGISSTNVGVMLHRA
                     KNKLSCLMKEAVHE"
     gene            2534..>3086
                     /gene="guaA"
                     /locus_tag="VG754_12140"
     CDS             2534..>3086
                     /gene="guaA"
                     /locus_tag="VG754_12140"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWF20014.1"
                     /translation="MEQIVILDFGSQYTQVIARRIRECQVYSVIVPFDKPATEIAAMK
                     PSGLILSGGPSSVYAKNAPLPDKRIFDLGIPTLGICFGVQLLAQFLGGKVEPGQKREY
                     GKGTLSVRDSNCPLFEGLPAKLQVWNSHGDKLTSLPKGFKPVATTENSDYAAIEDRKR
                     HFYGLQFHPEVAHTPQGRQIISNF"
BASE COUNT          693 a          736 c          906 g          751 t
ORIGIN      
        1 gaattgcgcg agtgaccgtc agtttggatc gcacccaaaa taagtattca gtgttcagtt
       61 ttcagtgttc agcaaaaggt cgtttcgttt ggcttggaaa ttttcaagtt ctttgtctcc
      121 acgcaaaacg tggtggaacg cgatcgggaa agttttggtg tcgtagttga aatccggcga
      181 ggcttgcacc tggatgtgga catgtgggcc actggtgttg ccggaatttc cgcattgggc
      241 gatgggttgg ccggccttta ctacgtcgcc aacttttacg gttggactgt tacgcttcag
      301 atgagcgatc agcacgtacc ggtcgggagc aatttctatt gtgatgtaat tgcccgcaac
      361 atttaccttg tcggtttggc cctttttgtt gtcgcggtga tttgattcca catggctgac
      421 gtggcctgag cacgggctgt aaatcgtttc gccgaaaccg gcatcgccct tcatgatttt
      481 ccaatcctta atctgctgca cagtgtcggc gactttgaaa atgtccaggg cgtcacgttg
      541 agagtgcagc agataatgat gattgatgat gacgtcattg ccgccttgaa gaacgcatgc
      601 gacgcctttg agaggggaat ccaagaggac cttttcggtt ttcaatggcg cagcaatttg
      661 gacgaagtgc cagatcatga aacacgcgcc gatggaatac atggcgataa atggaatcga
      721 gatgcgccga gcaggcatga gggcgaggac aatttgaagc gcggccagac tccatgtcac
      781 ggtttgcata tagtgccggg cgtgttgtgg ggattggcag ggcgcaatgg cgatgctcag
      841 caggtagccg cacgatgcga agaacgcgag gcgcacaaga gctgaaatcc agtacgtccg
      901 tgactgtttc gcaaatggat tgaacaacaa accatagtag atcttgccga gagcggcttt
      961 gaacacgaag accacaatgc tcaacggcac gagcagcatc agtagcatgt aaacgaaatt
     1021 cgtgaatatg ccgcctccgt atgcgaacaa gccggcgatg cataggaaga gcgcgagggt
     1081 gctttccgcg atgttgatca aaacaatttt gacgcggctc ggggctgcgg gttcggcggt
     1141 gatggatgcg atggtttggg tttgcataat gatttcgtcg aattgaccgg tttccatgcc
     1201 ttgaatgagc gcgtcgagtt gcgcgatcat cgggcgcagt tttttgcgaa gggcgtattg
     1261 attgcagaaa tataggacga cgtgtaaggc gagcactccg atggagaaat ctattttcca
     1321 cgacaattca cgccccggcg ttccccacgc gaagactaat gttccaatga acagagggag
     1381 caaataccac cagacgaccg tgcgtaagag ttcggtttga gaatgaacgg agcgacgttc
     1441 agcgtgcagg gattcaatga cggatgcgga tggttgggcg ggcgggttag cgttgcgcgt
     1501 gcggacgagt tttgcgccaa taaagactgc agcggcgatg agaatgatgt cgccaatgcg
     1561 cgtgacgagc gttgggatgc tgaagaaata atagccgaac ccaatgatga tgaaggcgca
     1621 tgcggcgagt tcgcgagcgt ctcgcgcgat gatttgacgg cgccatttcg acattttgcc
     1681 gcgcattttc gcggtcaaat cgtctgcgga tagagatgga gcagcgggct cggtctgctt
     1741 ctgccacaga tcctttaaat cctggtcatt catggacggc ctccttcatc aggcaggaaa
     1801 gtttgttttt ggcgcgatgg agcatgacgc cgacgttcgt cgagctaatg ccgaggactt
     1861 cggcgatttg gtcataggtg aaatcttcga gaaacagtgt aacgagggct ttttcgagtt
     1921 cgttgagttt ggcgatggca gcgtaaagat tggcgaggcg gtcgtccggc tgtggctcga
     1981 cggtttgttg agcttctatc gttcgtgcaa gttgtgtgtg atcgacgtgc aaaactcgat
     2041 caccgcgcga ggcgcgctta cgaacgaacg aaatggcagt gttgatggcc acgcgataaa
     2101 gccaggtgtt ggcgaattgt ttttctttca gggccggcaa gccgcgccag atttggaaca
     2161 cgatttcctg atataagtcg tcctggtcgg cggcgttcca cgcgtaaaca cgacagacct
     2221 tgagaatccg gtttcggttc tcgttcacca gttcgaggaa ctgttggtcg tcgggttgcg
     2281 ttggtttcaa ttcggccctt tatctgatta gtcgagagcc acgcgcggat tattacaaga
     2341 aaatcgaaga gttttagtcg tggtccgaca atccgtcttg tcgggatttc cttcgcgacg
     2401 aaatagccgg ttccgttttc ctcagcgcgc tgagccttgc ggacgaagtc accccactga
     2461 aaaactttaa ttcggcatca accatccgtt gacgagtcgc gctttagaca ttagcgttcc
     2521 cgcttctttc gacatggaac agatcgtcat tctcgatttc ggttcgcaat atacgcaggt
     2581 tatcgcgcgg cgcattcgcg agtgccaggt gtactcggtg atcgtgccat tcgataaacc
     2641 cgccacggag atcgccgcga tgaagccgag cggcttgatc ctctcgggtg gtccgtccag
     2701 cgtttacgcg aagaacgcgc cgttgccgga caagcgcatt ttcgacctcg gcattccgac
     2761 tctgggcatt tgtttcggcg tgcaactcct cgcgcaattt ctcggcggaa aagttgagcc
     2821 cggccaaaag cgcgagtacg gcaaaggcac cctgagtgtc cgcgattcaa attgccctct
     2881 tttcgagggg cttcccgcga aactccaggt ctggaattcg cacggcgata agctgacgag
     2941 tttgcccaaa ggatttaagc cggtggcgac gacggaaaat tccgactacg ccgcaattga
     3001 agataggaag cggcattttt acggcctgca atttcatccg gaagtcgcgc acaccccaca
     3061 aggtcgccaa atcatttcca atttcg
//
LOCUS       DASZMM010000506         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2691336, whole genome shotgun sequence.
ACCESSION   DASZMM010000506 DASZMM010000000
VERSION     DASZMM010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691336"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..949
                     /locus_tag="VG754_12145"
     CDS             71..949
                     /locus_tag="VG754_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWF20015.1"
                     /translation="MRLFEAIVDANHRALAGDATAGLHPADFADSLPVAALTCIDARL
                     NPLFPEVLGLPKEQFIWLRNAGNIVTGPLSSTMRSLALACAVKGGKEIAIIGHNDCLV
                     CKTSTAQLTDAFAALGVDRAGLPPNLTEFFGLFASERQNVLKAVEFVRHSPIIGSKVP
                     VHGLIVDIQSGKLEWVSNGYQTMGAAESFKAEGAKLMDETSDVLKSLPGFVIGEMKFP
                     ETKIGELATDARNWLSQEIGNLSSQPPAPSPVPGASNQPPPAPPIVRVAPPVAKRVPV
                     PPPVRPAYTYPKNKKF"
     gene            1006..1524
                     /locus_tag="VG754_12150"
     CDS             1006..1524
                     /locus_tag="VG754_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWF20016.1"
                     /translation="MSLFATFSLTAADNLIGTTPPEWHVTHWMNSAPLKLGDLRGKVV
                     LVRWWTAPECPYCRATAPALNEFYGNYHALGLEVLGFYHHKSPEPLEPDKVGKYAADF
                     GFKFPLAIDADWKTLHQWWLDSQKRNFTSVSFLIDRHGVIRHIHPGGEYVKGDKDYLA
                     MKAKIEELLAEK"
     gene            complement(1538..2395)
                     /locus_tag="VG754_12155"
     CDS             complement(1538..2395)
                     /locus_tag="VG754_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HWF20017.1"
                     /translation="MNGRVTFVKGQGGLPMLRIETPSSTAEIYLHGAHVTHFQRKNES
                     PLLWLSRESKFETGKAIRGGIPVILPWFGPRENAPAHGCARIKEGELVEISTANGGIS
                     VHMRLAHCAEMESFGAFTADHVVTVGDALELELTVANPSTDRELAIEDCLHTYFAIGD
                     ISEVAVAGLKGAKYLDKVGGSVEKTETNDVITFNSEVDRVYMDTTSPVEILDRKLRRK
                     IRVEKEGSSATVVWNPWIAKAKAMPDFGDEEYHEMVCVESGNVGKNHLTLAPGKSSTM
                     RVKLSTNSV"
BASE COUNT          595 a          754 c          726 g          624 t
ORIGIN      
        1 gaattgcgcc tattgtggag agcgggtttt cgagacgcca accaagcaaa accaatcttg
       61 atgcgatttt atgcgcttgt tcgaagccat cgtggatgcg aaccaccggg cgcttgccgg
      121 tgatgcgacg gcgggactgc atccggcgga ttttgccgat tcgttgccgg tcgcggcgct
      181 cacgtgcatt gacgcccggc tgaatccgct ttttcccgag gtgctcggcc tgcccaagga
      241 gcaattcatc tggctgcgca acgcgggcaa tatcgtcacc ggcccgttga gcagcacgat
      301 gcgctcgctc gcgctggctt gcgccgtcaa gggcggcaag gagattgcca tcatcggcca
      361 caacgattgt ttggtctgca agacttcgac tgcgcaatta acggatgcat tcgcggcgtt
      421 gggtgtggac cgcgccggtt tgccgccgaa tttgacggag tttttcggat tgttcgccag
      481 cgaacggcaa aatgttttga aagcggtgga atttgtgcgg cacagcccca tcatcggctc
      541 caaagttccc gtgcacggat tgatcgtgga catccaaagc ggcaagctcg aatgggtgag
      601 caacggttat caaaccatgg gcgcggcgga atcgttcaag gcggaaggcg caaagctcat
      661 ggatgaaaca tccgacgttt tgaaatcgtt gcccggcttc gtcatcggcg aaatgaaatt
      721 tccggagaca aaaattggag aacttgccac ggacgcgcgc aactggctgt cgcaggaaat
      781 cggaaattta tcgtcccaac cacccgcgcc gtctccagtt cccggagcgt cgaaccaacc
      841 accgcctgcg ccgcccattg tgcgtgttgc cccgcctgtg gcaaaaaggg tccccgtgcc
      901 gccgccagtt cggcctgcat atacttatcc aaaaaacaag aagttttgaa ctttctgatg
      961 caacgctcct gcaaattttt ttctgcgcgt acgatatggc tggcaatgtc tctctttgcg
     1021 acgttttctc tcactgccgc tgacaatttg atcggcacga cgcctcctga atggcatgtc
     1081 acccattgga tgaactcggc gccattgaag ctgggcgatt tgcgcggcaa ggtcgtattg
     1141 gtccgatggt ggacggcgcc ggaatgccca tattgccgcg cgacagcgcc agcgttgaac
     1201 gaattttacg ggaattatca cgcgctagga ttggaagttt tgggttttta tcatcacaaa
     1261 tctccggagc cgttggagcc ggataaggtc ggcaaatacg cagcggattt tggattcaag
     1321 tttccactgg cgattgatgc ggattggaaa actttgcatc aatggtggtt ggacagccaa
     1381 aaaaggaact tcaccagcgt cagtttcctg attgaccggc acggcgtcat tcgccacatc
     1441 catccgggcg gcgagtatgt gaagggcgac aaggattatc tcgcgatgaa agcgaagatt
     1501 gaagaacttc tggcggaaaa gtaggaatgc gttcgagtca gacagaattt gtggaaagtt
     1561 tcacacgcat ggttgaagat tttcccggcg caagggtgag atggtttttt ccgacattgc
     1621 ccgattccac gcaaaccatc tcgtgatatt cttcatcgcc gaaatccggc atcgccttcg
     1681 ctttcgcaat ccacggattc caaacgaccg tcgcggacga gccttccttt tcgacgcgga
     1741 ttttccggcg caatttgcgg tcgaggattt ccaccgggct ggtcgtgtcc atgtaaacgc
     1801 ggtccacttc agaattgaaa gtgatgacgt cattcgtctc ggtcttctcc accgaaccgc
     1861 cgaccttgtc caaatacttc gctcccttta aaccggcgac cgcgacttcg ctgatgtctc
     1921 cgatggcgaa ataggtgtgc agacaatctt caatcgccaa ctcgcggtca gtggagggat
     1981 tcgccacagt caattcaagt tccaacgcgt caccgacggt gaccacatga tcagccgtga
     2041 acgcgccgaa cgattccatc tccgcgcaat gcgccaaacg catgtgaaca ctgataccgc
     2101 catttgccgt ggatatttcc accaactccc cttccttgat acgcgcgcaa ccatgcgctg
     2161 gcgcattctc gcgcgggccg aaccatggca ggatgacggg aattccgcct cgaatcgcct
     2221 tgccggtttc aaacttgctt tcccggctca accacaaaag cggcgattca ttcttgcgtt
     2281 gaaaatgtgt gacgtgcgct ccatgcagat aaatttccgc cgtgctcgaa ggtgtttcaa
     2341 tcctgagcat cggcaaccca ccctgccctt tcacgaaggt gacacgccca ttcattccgc
     2401 aagctgactt ctgatgcgca aggatgtcaa gagagatggg aaagatcgga gcgcggctgg
     2461 gacagccgca ggaatgttaa aacgttcggt cggttgggat tagtctaatc ctgtctgcac
     2521 tcaccacgtt gctgcggctg ggacagccgc gctccagaaa gtcgtcatgc cctttttcat
     2581 ttacaattct ttacactcac cgctaggctc gcagcactat gccacgcaat tacaaatttt
     2641 gtttcggccc atggaacatc agcgaaggtt ccgatcctta cggcccgacg acacggccc
//
LOCUS       DASZMM010000507         5510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_783069, whole genome shotgun sequence.
ACCESSION   DASZMM010000507 DASZMM010000000
VERSION     DASZMM010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783069"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VG754_12160"
     CDS             <1..799
                     /locus_tag="VG754_12160"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWF20018.1"
                     /translation="SGKTLGEVLDWWEGSERRARFRELLRKLDGVEADDVILPPEKAR
                     EQGLTSTMIFPRGNLAPDGSVIKSTALDPSLVGADGIYFKEGRARVFVSEAAAMKAIK
                     QKQIQPGDVLVLAGIGPMGTGMEETYQVTGALKYLPFGKEVALLTDARFSGVSTGACI
                     GHIGPEGLAGGPIGKLLDGDLIRIVINRKENIGSVDFVGEGDRKVSPAEGAEILSRRA
                     SRKDLAPNPALPDDTRLWAALQSVGGGTWGGCVYDTERIVKLLLSHG"
     gene            1134..1697
                     /locus_tag="VG754_12165"
     CDS             1134..1697
                     /locus_tag="VG754_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20019.1"
                     /translation="MHRVAWLPNGRFPVNAVKAWSSHAKIQTTDYQSTPYSNFGPNES
                     RRVKPLFGASQGTIDAKSTKDWRTPRRYRDIKQRRNAARFCTAAVHNSSIVSFQLVLD
                     GGVGSPKHGRQSLVKPWPTIKKPHFVAVRLTSAQINRDKPGYGKQIFSHNHQAAALKI
                     IARVPSRAPIETSASFGTWRAATGQIG"
     gene            1708..3210
                     /locus_tag="VG754_12170"
     CDS             1708..3210
                     /locus_tag="VG754_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWF20020.1"
                     /translation="MNMNLATAFEKSAQQSPQKTALYWGEREYSFQELLTETAKVASY
                     LRNKVGVEPGDRVALWLKNCPEFIPALFGILQVGGVVVPINNFLKPAEVSYIVGDAGA
                     NVLLTDATMAEHLPALMELKPDLKCVQVEAFTTHEDGGAMQPSDRQVEDLAVIIYTSG
                     TTGRPKGAMLSHGNLLHNVESCRLVLRCVNEDRVAVLLPMFHSFMLMVGVFLPMLVGG
                     SIVLIRSLHPPRNVLQEINQRQATILPAIPQFFRTMASSPVPISLPSLRMCISGAAPL
                     PVQVLTEFAAKFPFPLIEGYGLSEASPVVTKNPLVGVRKPGSIGLPIPNVEVSIQDDE
                     GKNLGPGEIGEVCVRGGNVMVGYWNQPEETAKAMRGKWLLTGDIGYRDADGYFYITDR
                     KKDMLLVNGINVYPREVEEILYQFPGVKEAAVIGVPDARKGEQPIAFVSANEGASLDE
                     KGLQQFVRSKLADYKVPRRVVLMPALPRNATGKILKTALREMAVKTSGNE"
     gene            3496..3636
                     /locus_tag="VG754_12175"
                     /pseudo
     CDS             3496..3636
                     /locus_tag="VG754_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301710.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
     gene            3708..4880
                     /locus_tag="VG754_12180"
     CDS             3708..4880
                     /locus_tag="VG754_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWF20021.1"
                     /translation="MSQQLAYVIITPYTLHKSRTGGILSRLITRTGLEMVGARMFAPS
                     AELVQKYSEAIVSANDPQDRRIQELIYNYILQNLGPDPKTGKRRRIMMLLLKGEDAVR
                     KTRSVIGNINAERCSGETIRDTYGDLVMDEKGQVKYFEPAVLAAPNVEEAEVKAKLWA
                     HYSDADGGIVENTLNYPAGERPQRTLVLIKPDNFRFPTGRPGNVIDFFSRTGLFIVGI
                     KVHRMSVAEATEFYGPVKDVLRTRLKDVVASKAKSAIEKELGFKVPPTEEKQLGEILG
                     PIFGDNQFENIVKFMSGRAPSECDASQMNQPGTEQCIALVYEGVEAVRKIRDVLGPTD
                     PSKAPPGSIRREFGQTIMVNAAHASDSEENAQRELKIVRVGENNFRKVVEEFYGKS"
     gene            4888..5097
                     /locus_tag="VG754_12185"
     CDS             4888..5097
                     /locus_tag="VG754_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20022.1"
                     /translation="MNAAQVIDEIKHLPQVEQAEVIQFAFELARSRQLAPRELGEMAE
                     QLAKSNDPAEIVRLKSAMTRGFYGE"
BASE COUNT         1339 a         1377 c         1638 g         1156 t
ORIGIN      
        1 ttcaggaaaa actttgggcg aagttttgga ttggtgggaa ggctctgaac ggcgcgcgcg
       61 tttccgcgaa ctgttgcgca aattggacgg cgtggaagcc gatgatgtca ttcttccgcc
      121 agagaaggcg cgtgaacaag ggctgacgag cacgatgatt tttccacgcg gcaatcttgc
      181 cccggacggt tcggtcatca aaagcacggc gctcgatcca tcgctggtcg gcgccgatgg
      241 aatttatttc aaggaaggac gcgcgcgggt ttttgtcagc gaagcggcgg caatgaaagc
      301 catcaagcaa aagcaaattc aacccggcga tgtgcttgtg ctggcgggca tcgggccgat
      361 gggcaccggc atggaagaaa cgtatcaagt gaccggcgcg ttgaaatatc tgccgtttgg
      421 aaaagaggtc gccttgctga cggatgcgcg gttttccggc gtttccaccg gcgcgtgcat
      481 cgggcacatc gggccggaag gtttggcagg cggaccgatc ggcaaattgc tcgatggcga
      541 tctcatccgc atcgtgatca atcgcaagga gaacatcggc agtgttgatt tcgttggtga
      601 aggcgaccga aaagtttctc ccgctgaagg cgcggaaatt ctttcacgcc gcgcgtcgcg
      661 taaagacctt gctcccaatc cggcgcttcc cgatgacacg cggctttggg cagcgcttca
      721 gtcggttggc ggcgggacgt ggggcgggtg tgtttatgat acggaacgca tcgtgaaatt
      781 gttgttgagc cacggatgaa acacagatga aacacggaaa gaagcaaaaa tttaacaacg
      841 aatgcgttga aacccggtgg agtccctccg atcaaaccgg cccctcattt cctcatccgt
      901 gttcagtcca tgtttcatcg gtggctgatc tactgttatg gccaataaaa aacccgcccc
      961 tccttgggaa gcgggtaaac gccatttttc gaaaatttat gtaacagcaa acggtgcgtc
     1021 tcaacatggg gtgtaacccg caataaagga tgcttaaacc acggatgaac acgaatgaac
     1081 acggattcag agacacggat gctttgtaga aaacatatct ccatcctcgc tttttgcacc
     1141 gtgtcgcttg gttgccaaac ggacgctttc cggtcaacgc agtcaaggct tggtcaagtc
     1201 atgccaaaat ccaaacaacc gattatcaat caacgcctta ctccaatttt ggcccaaacg
     1261 agtcaaggcg agtcaagcca ttatttggag cgagtcaagg cacgatagat gcaaaaagca
     1321 ccaaggactg gcgcactcca agacgctatc gcgacatcaa gcaacgtcgg aatgccgcgc
     1381 ggttttgcac tgcggcagtc cacaacagct caattgtttc gtttcagctt gttttagatg
     1441 ggggggtggg atcgccaaag catgggcgcc aaagcctcgt caaaccatgg ccgaccatca
     1501 aaaaaccgca ctttgtcgca gttcgtctca catcagctca gatcaaccgg gataaaccgg
     1561 gctatggaaa acaaattttt tcgcacaacc atcaggccgc cgccctgaag ataatcgcgc
     1621 gtgtgccgag tcgcgccccc atcgaaacat cggcctcttt tgggacttgg cgggcggcaa
     1681 caggccagat aggttgactc cttactcatg aacatgaatc tggcaacggc atttgaaaaa
     1741 tcggcgcagc aaagtccgca gaagactgct ttgtattggg gcgagaggga atattcattc
     1801 caggaattgc tcacggaaac cgccaaggtc gcgagttatc tgcggaacaa ggtcggggtt
     1861 gaaccggggg accgcgtggc gttgtggctg aagaattgtc cggaattcat cccggcattg
     1921 ttcggcattt tgcaggtcgg cggggtggtg gtgccgatca acaactttct caagcctgcc
     1981 gaggtttcct atatcgtggg cgatgccggg gctaatgtgc tcctgacgga cgcgaccatg
     2041 gcggaacatt tgcccgcttt gatggaattg aagccggatt tgaaatgtgt ccaggtggaa
     2101 gccttcacca cgcacgaaga cggcggggcg atgcaaccgt cggaccggca ggtcgaggat
     2161 ttggcggtga tcatttacac ctccggaacg accgggcggc ccaagggagc gatgctttct
     2221 cacggcaacc tgttgcataa cgtggagagt tgccggttgg tattgcgctg cgtgaacgaa
     2281 gaccgggtgg cggtcctgct gccgatgttc cacagtttca tgcttatggt gggcgttttt
     2341 ctgccgatgc tcgtcggagg ttcgattgtg ctgatccgtt cgctgcatcc gccgcgcaat
     2401 gttttgcagg aaatcaacca acgccaagcg acgattttgc cggcaatacc ccaatttttc
     2461 cgcacgatgg ccagttcgcc ggtgccgatc tcgcttccgt cgctgcggat gtgcatcagc
     2521 ggcgcggcgc cattgccggt gcaggtattg accgagtttg cggcaaaatt tccgtttccg
     2581 ttgatcgaag gctacgggtt gagcgaggca agcccggtcg tcaccaaaaa tccgttggtc
     2641 ggagtgcgca agccgggttc gattggattg ccaattccca acgtggaggt gagcatacag
     2701 gatgacgaag gcaaaaattt ggggccgggg gagattggcg aagtttgcgt gcgcggcggc
     2761 aacgtcatgg tcggttattg gaaccagccg gaggaaactg ccaaggcgat gcgcggcaaa
     2821 tggcttttga ccggggacat cgggtatcgc gatgcggacg gttattttta catcaccgac
     2881 cgcaagaagg acatgctgct ggtcaacggc atcaatgtct atccgcgcga ggtggaggaa
     2941 attttgtatc aatttccggg cgtgaaggaa gcggcggtga tcggcgtgcc ggacgcgcgg
     3001 aaaggcgagc aaccgattgc ttttgtgtcg gcaaacgaag gcgcgtcgct cgacgaaaag
     3061 ggattgcaac aatttgtccg ctcaaaattg gccgactaca aggtgccccg ccgcgtggtc
     3121 ttgatgcccg cgttgccgcg aaatgcgacc ggaaaaattc tgaaaaccgc gctgcgcgag
     3181 atggcggtga aaacgagcgg gaatgaataa aagctcctac attcaaactc caagcaccag
     3241 agaagcatca aaccacaagc tccaagccga tccggttgtc gcttggagct tgaagtttct
     3301 ctggagcttg gaggttggaa ttaggggctt tcgggcagca gcgttaaaac ctaaatactt
     3361 gtcaaccaaa taattgcatt atgaaatcag gtgttcaaga ggcgtccctt taccagttga
     3421 cggtggggag gaacgaaccg taaggtattt ccattaactg ccgggcgcat gatgcgtcag
     3481 acagggaaac tttctatgcc tgaaggatat tgcgtcaaat gcaaagccaa aaaggaaatt
     3541 gtaaatgcag tggaagaagt catgaaaaat ggaagaaagg ccatcaaggg cacatgtccg
     3601 acgtgcggga cggtgatgtt taagattctg gggggcaagg ctgcgacaac gccgcctgcc
     3661 gagccggtgc cggccaagcc tgcaacgtcg aattgaagcg ctgcattatg tcccaacaac
     3721 ttgcctacgt catcataacg ccctacacgc ttcacaagtc gcgcacgggc ggcatcttaa
     3781 gccggttgat cacccgcact ggattggaaa tggtgggagc gcgcatgttc gcgcccagcg
     3841 ccgaactggt ccaaaaatat tcggaagcga tagtttccgc caacgatccg caagaccggc
     3901 gcatccagga actgatttac aattatattt tgcaaaacct tgggcctgat cccaagaccg
     3961 gcaaacgtcg ccgcataatg atgctcttgt tgaagggcga ggacgccgtg cgcaagaccc
     4021 ggtcggtcat cggcaatatc aacgcggaac gttgctccgg ggaaaccatc cgcgacacct
     4081 acggcgacct ggtcatggac gaaaaaggac aggtgaaata tttcgagccg gcggtgctgg
     4141 cagcgccgaa tgtcgaagaa gccgaggtga aggcgaagtt gtgggcgcat tattcggacg
     4201 cggacggggg cattgtggaa aacaccctca actacccggc aggcgaacgg ccgcaacgca
     4261 cccttgtctt gatcaagccg gacaatttcc gtttcccgac cggccggccc ggcaacgtga
     4321 tcgatttctt ttcgcgcacc gggcttttca tcgtgggcat caaggtgcac cggatgagcg
     4381 tcgcggaagc cacggaattt tacgggccgg taaaggatgt tttgcggacg cggttgaagg
     4441 acgtggttgc gtcgaaagcg aaatcggcga ttgaaaaaga attgggattc aaggtgcccc
     4501 cgacggagga gaagcagttg ggcgaaattt tgggcccgat atttggcgat aatcaatttg
     4561 agaacatcgt gaaattcatg tcgggtcgcg cgccttccga gtgcgatgcg agccagatga
     4621 accagccggg gacggaacaa tgcatcgcgc tggtttacga aggcgtggaa gcggtgcgga
     4681 aaatccgtga cgtgctcggg ccgactgatc cttccaaggc gccgcccggt tccatccgcc
     4741 gcgagttcgg ccagaccatc atggtgaacg cagcccatgc gagcgattcc gaggaaaacg
     4801 cccagcgcga attaaagatt gtccgcgtcg gcgagaataa tttccggaag gtcgtggagg
     4861 aattttacgg gaagtcgtga gtaaaatatg aatgcagcgc aggtcattga cgaaatcaaa
     4921 catttgcccc aggtcgaaca ggcggaagtg atccagtttg catttgaact tgcccgcagc
     4981 cgccagcttg cgccgagaga acttggagaa atggctgaac aactggcgaa atcgaacgat
     5041 ccggcggaga tagtccgttt gaaatcagca atgacgcgcg gattctatgg cgagtgagcc
     5101 atgccaaaaa tcctccgcag gaatctaccc cgtccgccat acgctcattt attggagaga
     5161 atccaacaga gacaaatttc aggccagcaa cttgtcttga tgattgaatg gttggataaa
     5221 gaaccagaag tttccgatgg acaatggttc aagaggtttc ccagcatgat tgtatgcggc
     5281 gagggcgagt tggtgaagac ctttctgacg cccgaacagt cgccaactgg aaaggatgtt
     5341 gaataatgag atctgcgttc ttatctccaa agatcgggag ttgtttgcaa cttttgcagg
     5401 agcagggtgt cggcgggagg aaagccgtag ttcgccaact ggggacggcc aatccatgcg
     5461 aaatcttcgc agccgacggc gcgcggttcg tttttcaacc aatggcagcg
//
LOCUS       DASZMM010000508         7953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_783702, whole genome shotgun sequence.
ACCESSION   DASZMM010000508 DASZMM010000000
VERSION     DASZMM010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783702"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VG754_12190"
     CDS             <1..1183
                     /locus_tag="VG754_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HWF20023.1"
                     /translation="TVGTDTGHQDQNGVSAARALDNVEAKLNFGYLAVHRTAEVGKAI
                     IRNYYGANAAYSYFLGCSRGGGQAMMEAQRYPKDFDGIVAGAPAFDWTGLAANAVAIQ
                     KVLYPDSKQLDKTALDIEAMKKLQQAILDQCDGQDGLKDGVVQDPPSAHFDLAKVSGL
                     TGEQRKAVAAIYAGVRDDKGLIYPGYSPGAECDPQQWFAWIVGPVPGTAKNHMPDLMF
                     GFGTQIFKYLVFNDPDWDYSTYDYSNFKKDTRLAASVLNATNADLAGFKAHKGKLIIW
                     HGWADPALPAQATVDYYRQVQALDPKAAEYCRLFMVPGCLHCGGGPGATEVDWRSFIV
                     NWVEHGNAPDRLLARKTDHGKTQMTRPLFSYPTTAIYKGNGDPASAESFVAKEAPTAG
                     H"
     gene            complement(1237..2688)
                     /locus_tag="VG754_12195"
     CDS             complement(1237..2688)
                     /locus_tag="VG754_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF20024.1"
                     /translation="MSHHAPNPEHLALTRRDFLRRCGMGMGAIGLAGLLGNLGFLNSA
                     RAETSINPLTPKPPQFPGTAKRVVHIFANGGPSHVDTFDSKPALEKFAGKLLPMENLP
                     TERKTGAAFPSPFKFKKYGRSGIEVSELFPHVAENIDDICVIRSMAADVPNHEPSLLL
                     MNCGDARQIRPSMGSWVTYGLGSENQNLPGFIAMCPGGYPIQESQNWQAGFLPGAYQG
                     TYVNTEHTRIEELIENIKNNYTSLPEQRQQLDLLQKLNERHLQKRKDDAQLESRIQSF
                     ELAYRMQMDATDAFDISREPEHIRKMYGEGTQARQLLITRRLLERGVRFVQVWHGAGQ
                     PWDSHDDIQVNHGNLARDADQAIGAFLTDLRQRGMLEDTLVIWGGEFGRTPTVELPKP
                     GLNAGHTNGRDHNHHGFTMWLAGGGARGGYVHGATDEYGFGAVENKVHVHDLHATILQ
                     LLGFDHEKLTFRHSGRDFRLTDVTGRVVRELIA"
     gene            2979..3242
                     /locus_tag="VG754_12200"
     CDS             2979..3242
                     /locus_tag="VG754_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20025.1"
                     /translation="MAGCKTQVCDTRGQSEVCEIHHQFMQTEVVDNKKNWRMPSQEYL
                     EARAHFFRHSYPFVLPEKCKKCAIYVCEDCVRAEEEWKRKHGQ"
     gene            3648..4799
                     /locus_tag="VG754_12205"
     CDS             3648..4799
                     /locus_tag="VG754_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF20026.1"
                     /translation="MPSEMSIEVMSGADLKGLLQGGLNDLRQIEFKPALPLPTDAGRE
                     EFLADIASFANASGGHIFYGVTAGLEKSAVEQAKTWLELAVVTGISPRIPRLLFKTLE
                     LPDNRCLVAIRIPKTWIGPHMVVFRQANRFYSRTSTGRCLLDVGGLRSAFAMNESFAE
                     KMRSFRMDRVNAILNRSLTVKLSESPKTVLHLFPVASFQPGFRVDLERIESGETDAPM
                     PMGARRLMNHYNYDGLITFSSAEKQAYTYLQIFRNGCFEAVESQLLEPHDGRKFFPGA
                     AFEKEVIQCGERLLKLLHMLEIEPPFVAMLNYLGVRGYGMSAGPMRWHTSGHEIERDH
                     LFLDEVVIADFAQDFSRAMRPAFDQVWNSCGWSKSLNYDQDGNWREATH"
     gene            complement(4803..5018)
                     /locus_tag="VG754_12210"
     CDS             complement(4803..5018)
                     /locus_tag="VG754_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20027.1"
                     /translation="MKTKQKWVWWLISALALGSVAGCATDNDRYQGGPGPGYGYQQGQ
                     PGYWNDWNNPGPPYEWQHPSPREQMTP"
     gene            5182..6435
                     /locus_tag="VG754_12215"
     CDS             5182..6435
                     /locus_tag="VG754_12215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HWF20028.1"
                     /translation="MRSKFSVRNARNPVLGQFGTFDVENYGDLLYPVLFKKMLEQRRK
                     NGDVAKFSIRGSNSLQDSGYRTRPVRDLFSRRGERPGTLVVGGGDLLHIQWDTVVSYY
                     RSLCREEGEDLRPGRWRRRWLRLVNKPRDEDTEFRRRYLNYPAVGPFMIKPNRLIKSV
                     AYCSCGVPSPFDEIVGREVANTFDRASFIYVRDNQSKENLTRAGVEKEIHVAPDLIVA
                     LSDFFDGAVEREKGRKLLREGGINTDRPILIFQCHVQAPEFADKIVEQLKAHQARNDC
                     EVVLVPLGGCHGDREFLKDLAQRSAGAFKYVELRSIFDIISVLAACDVFAGTSMHGNI
                     TAFSFGIPHVFGPLNLTKREGFLEIAGLPQDVKLETWEELNAALELALNRGSGFFQNR
                     LDTAKQRVNEVFDKLCRAINAEERH"
     gene            complement(6492..6677)
                     /locus_tag="VG754_12220"
     CDS             complement(6492..6677)
                     /locus_tag="VG754_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20029.1"
                     /translation="MKAYKKWIWGLLGAIALGMISGCVVGPDDDHYRGGYNGEYGHGY
                     GYEHHDYDHDRYNHDWH"
     gene            6905..7843
                     /locus_tag="VG754_12225"
     CDS             6905..7843
                     /locus_tag="VG754_12225"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HWF20030.1"
                     /translation="MAATLVLGAGCRTCNRESSANYAPISTGGMGNEYGTESGQYSSG
                     GDVVIPLEKEQAQVGTQRVDQGGVRIRKFVRTETINEPVQVREETVSVDRVPGGAQGN
                     ANTALNTPFQSGEITIPLVKEQPVVTTQVVPDGAVVIHRQETTQQMNVPAQVRQERVV
                     AEPVGNPANLNISQDLRRGERENEEPYAQGAPGASYGQSSGTSGQPITQLNQLCNTSD
                     TASLYNSQVNISNVKVDRVLNDQLIQVRADNGTKFFVHIDQPTQGLHRDQIITLNGAI
                     KQVPSDPSTLGWDPASAQAVQGQQLVIDCPSVSPQG"
BASE COUNT         1841 a         2092 c         2231 g         1789 t
ORIGIN      
        1 gacggttggc acagataccg ggcatcaaga ccaaaacggt gtttctgccg ctagggcgct
       61 ggacaacgtg gaggcgaaat taaatttcgg ttatctcgcg gtgcatcgca ccgccgaggt
      121 gggcaaggcc atcatccgca attattacgg cgctaacgcc gcttactctt attttctcgg
      181 ctgctcacgc gggggaggcc aggccatgat ggaggcgcaa cgttacccga aggatttcga
      241 cggcattgtc gccggtgccc cggctttcga ttggacgggc cttgccgcga atgcggttgc
      301 gatacaaaag gtgctgtacc cggattcgaa acagctcgat aaaaccgccc tcgatattga
      361 agccatgaag aaattgcagc aggcaatcct tgaccaatgc gacgggcagg acggccttaa
      421 agatggcgtc gtgcaagacc cgccttccgc gcacttcgac ctggccaaag tatcgggatt
      481 gaccggggaa caacgcaaag ccgtcgcggc catctacgcc ggagtccgcg acgacaaagg
      541 tctgatttat cccggctact cacccggcgc ggaatgcgat ccccaacaat ggttcgcgtg
      601 gattgtcgga ccagtaccgg ggacggccaa aaaccacatg cccgatctga tgttcggctt
      661 tggcacgcag atattcaaat acctcgtttt taatgatcct gattgggatt actcgaccta
      721 tgattattca aatttcaaaa aagacactcg cctggcggca tcggtcctga acgcgaccaa
      781 tgccgacctt gccgggttca aggcgcacaa aggcaaactc atcatctggc acgggtgggc
      841 cgatcccgca ttaccggcgc aagccacggt tgattattac cgtcaagtgc aggctctcga
      901 tccgaaagcc gcggaatatt gccggctgtt catggtgccg ggttgtttgc attgcggcgg
      961 agggccaggc gcaaccgagg tggactggcg ttccttcatt gtgaattggg tcgaacacgg
     1021 caatgcgcct gaccggctgc tcgcgcgcaa aaccgatcac ggcaaaacgc agatgaccag
     1081 gccgcttttt agctacccaa cgaccgcgat ctacaaaggc aacggcgatc ctgccagcgc
     1141 tgaaagcttt gtcgccaagg aagccccgac tgcggggcat tagtgcggtt tcataaatta
     1201 acaggctgaa tcgctgttcc tgcgtaaaaa gaaattttac gcaatcaact cgcgcaccac
     1261 ccgccccgtc acatcagtca aacggaaatc gcggccggaa tggcggaagg tgagtttctc
     1321 atgatcgaag ccgagcagtt gcaaaatcgt cgcgtgcagg tcgtggacat ggactttgtt
     1381 ttcaaccgcg ccaaagccat attcgtccgt cgcgccgtgg acgtatccgc cgcgcgcgcc
     1441 gccgccggcc agccacatgg tgaacccatg atgattatgg tcgcgtccgt tcgtgtgtcc
     1501 ggcgttcaag cccggtttgg gcaattcaac cgtcggtgtg cggccaaatt ctccgcccca
     1561 aatgactaaa gtatcttcga gcatcccgcg ctggcgcaaa tcggtcagga acgcgccgat
     1621 ggcctggtct gcatcgcgcg caagattgcc atggttcacc tggatgtcgt catggctgtc
     1681 ccaaggctga cccgcgccgt gccagacctg cacgaaacgc acgccgcgtt ccagcaaccg
     1741 ccgcgtgatg agcaattgcc gcgcttgcgt tccttcaccg tacatcttgc ggatgtgctc
     1801 cggctcgcgg ctgatgtcaa aggcatccgt cgcatccatc tgcatccggt aggcaagctc
     1861 gaaggattgg atgcgtgatt ccaattgcgc gtcgtccttg cgtttttgga gatgccgttc
     1921 gttcagcttt tgaagcaggt cgagttgctg gcgttgttcg gggagcgagg tgtaattatt
     1981 tttgatgttc tcgatcaact cctcgatccg cgtgtgctcg gtgttgacgt aagtgccctg
     2041 gtaagcgccg ggcaaaaaac cggcctgcca attctgcgat tcctggatgg gataaccgcc
     2101 cggacacatg gcgataaagc ccggcaaatt ctggttttcc gacccgagcc cgtaagtgac
     2161 ccacgaaccc atgctgggac ggatctgacg cgcatcgccg caattcatca ataacaacga
     2221 cggctcatgg ttcggcacgt ccgcggccat ggaacggatg acgcagatgt cgtcaatgtt
     2281 ttcggcgacg tgcgggaaaa gttcgctcac ctcgatgccg ctcctgccgt atttcttgaa
     2341 cttgaacggg gacggaaatg ccgcaccggt cttgcgctcg gtcggtaaat tttccatcgg
     2401 caaaagtttg ccggcaaatt tttcgagcgc gggcttggaa tcgaaagtgt caacgtgcga
     2461 aggcccgcca ttggcaaaaa tgtggacgac gcgtttggcc gtgccgggaa attggggcgg
     2521 cttgggcgtg agcggattga tgctcgtctc ggcgcgcgcg gaatttagga aaccgaggtt
     2581 gccgagaagc ccggcgaggc cgatggcacc catgcccatc ccgcaccgcc gcaagaaatc
     2641 gcggcgcgtc agcgcgaggt gctcagggtt gggcgcgtga tgactcatct gaatctaact
     2701 atagcatgat tgacgaaccg gaggaacgaa aaattcgatt gaaatgccca aatacccaag
     2761 gaaatcccaa gcacgaatcc ccgatgccgg atcattttga cagaatgaac agaatggaac
     2821 ggtaaaatcc tgttttcatt cttaacattc tgttcattct gtcaaaatgg tcccgggtat
     2881 tttgatagac actttttacc gggcgaaata ggatgcaaac catgtttgag aaagcgcgtt
     2941 tattttccgt ctgtgttctc gtggttttga gttttgcgat ggctggatgc aaaacacagg
     3001 tctgcgacac gcgcggtcaa tcggaggttt gcgaaatcca tcaccagttc atgcagacgg
     3061 aggtcgtgga caacaaaaag aactggcgga tgccgtccca agaatacctt gaggcgcgag
     3121 cccacttttt tcggcacagc tatccgttcg tcctgcctga gaaatgcaaa aaatgcgcca
     3181 tctatgtctg cgaagattgc gtgcgcgcag aggaagaatg gaagcgcaag catggccaat
     3241 agtttacgag cagcaatcgg gctttcggcc aattgcagca gtttaaataa aggcgcgaac
     3301 tttgggccga ggcattgaat cctcaacatt tatccgactt cacgacaagc atgtctccca
     3361 aaagagaacc cggaaaaatg cgacgtgcct ctggcctcag ccgtattctt gagcagcaac
     3421 tcaatatgaa aggccaaact ccagccgcag gctggaaagc ctgcgccacg tagcgcagac
     3481 attcttgtct gcgggttcag gcgactttcc agtccccgct ccacaacaat gccaagatgc
     3541 ccattgggta tctttccaat tttctgattg tgcggcggta tctgttttgc taatctggat
     3601 tgttattcgc gtttgttagt ccagtggttc agcgagtact cccaaccatg ccatctgaaa
     3661 tgtcaatcga agtgatgtcc ggggctgatc tgaaagggct gcttcagggc ggattgaacg
     3721 acctgcgcca gattgaattc aagcccgcgt tgcctcttcc caccgacgca ggccgcgaag
     3781 aatttcttgc ggatatcgcc tcgttcgcca acgcgtcggg cggccatatt ttttatggcg
     3841 tgaccgcggg gttggaaaag agcgccgtgg aacaggccaa aacatggctt gaactggcgg
     3901 tcgtcaccgg catttcgccg cgcattccac gcttgctttt caagacgctt gaattgccgg
     3961 acaatcgttg cctcgtggcg atacgcattc ccaagacatg gatcggcccg cacatggttg
     4021 tgttccgaca ggcgaaccgg ttttactcac gcacttcaac gggcagatgc ctgctcgatg
     4081 tgggcggatt gcgctcggcg ttcgcgatga acgaatcgtt tgccgagaaa atgcggtcgt
     4141 tccggatgga ccgcgtgaac gccatcttga accgctcgtt gacggtgaag ttgtccgaat
     4201 cgcccaagac tgttctgcat ctgtttcctg tcgcttcctt tcaacccggg tttcgcgtcg
     4261 atttggaacg catcgagtcc ggagaaacgg acgcaccgat gccgatgggc gcgcgtcgtt
     4321 tgatgaacca ttataattac gacggattga tcacgttcag ttcggcggaa aagcaggcct
     4381 acacttatct gcaaattttt cgcaacggct gttttgaagc ggtggagagc cagttattgg
     4441 aaccgcatga tggccgaaaa tttttccccg gcgcggcatt tgaaaaagaa gtcatccaat
     4501 gcggtgaacg attgttgaag ttgctgcaca tgctcgaaat tgagccgccg tttgtcgcga
     4561 tgctcaatta tctgggtgtt cgcggttatg ggatgtcggc agggccaatg cgctggcaca
     4621 ccagcggcca tgaaatcgag cgcgaccatc tgttcctgga tgaagtggtc atcgctgatt
     4681 tcgcgcaaga tttctctcgg gccatgcgcc cggccttcga tcaagtctgg aattcttgcg
     4741 gctggtcgaa atctctcaat tacgaccaag atggcaactg gcgcgaagcg acgcattgag
     4801 catcaaggcg tcatttgctc ccggggagaa ggatgttgcc actcgtaagg cgggccggga
     4861 ttgttccaat cattccagta tcccggctga ccttgctgat acccataacc gggtccgggg
     4921 ccgccttgat agcggtcgtt atcggttgcg catccggcga ctgagccaag cgcgagcgct
     4981 gatatcagcc accataccca tttttgtttt gttttcataa atgatgatct gtgattggat
     5041 ttgggcgttg ccttcttact aaaagaaaaa cccacaatcg ccttccggca atggggtatc
     5101 ttccccgata ggtgttcaaa aggccgaagt gggctgcttg actttgcgcc gctgaaggct
     5161 tcaaaatgcc tgaaaatgac ggtgcgttcg aaattttcgg tccgcaatgc gcgtaatcct
     5221 gtccttggcc aattcggcac ttttgacgtg gaaaattacg gggatcttct ttaccccgtc
     5281 cttttcaaaa aaatgctgga gcaacgccga aaaaatgggg acgtggcgaa attttcgatt
     5341 cgcggaagca attcgctgca ggattcgggt taccggacac gtcccgtgcg tgacttgttt
     5401 tcgcgacgcg gagaacggcc tggaacgctt gtcgttggcg gcggggattt gctgcacatc
     5461 caatgggaca cggtggtttc gtattatcgt tcactttgcc gggaagaagg cgaagattta
     5521 aggccgggcc gttggagacg gcggtggttg agattggtga acaagccgcg ggatgaggac
     5581 acggaattcc gcagacgcta tttgaattat cccgcggtcg ggccgttcat gataaagcca
     5641 aacaggttga tcaagtcagt ggcgtattgt tcttgcggcg ttccatctcc gttcgatgaa
     5701 atagttggca gggaagtcgc caacactttc gatcgcgcat cattcattta tgtgcgcgac
     5761 aatcaatcga aggagaatct gacgcgtgcc ggtgtcgaaa aagagatcca tgtcgcgcct
     5821 gatttgattg tggcgttgag cgattttttt gatggggctg ttgagcgcga aaagggaaga
     5881 aaactccttc gcgaaggcgg tatcaacacg gaccgtccca ttctgatttt ccaatgccac
     5941 gtgcaggcgc cggaatttgc ggataaaatt gtcgaacagc tcaaagctca tcaagcgcgc
     6001 aacgattgcg aggttgtcct tgtgccattg ggcggatgcc acggcgaccg ggaatttctc
     6061 aaagaccttg ctcaacgctc cgcaggcgca ttcaagtatg tcgagttgcg ttccatcttt
     6121 gacatcatct ccgtccttgc tgcgtgcgat gtttttgcgg gaacaagcat gcacggcaac
     6181 atcacggcat tttcatttgg gattccgcat gtgttcggtc ctctcaatct tacgaaacgc
     6241 gaaggatttt tggaaatcgc gggtctgcct caagacgtca aactggaaac ctgggaagaa
     6301 ttgaatgccg cactggagtt ggcgctcaat cgcggcagcg gcttttttca aaaccgcttg
     6361 gacacggcaa aacaacgcgt taatgaagtc ttcgataaac tgtgccgggc aatcaatgcg
     6421 gaagaacgcc attaattttt tttgaaaaaa gccggggagt ggttgcgccc cggcttgttc
     6481 agacagacag attaatgcca atcatgattg taacggtcat ggtcataatc atggtgttcg
     6541 tatccgtaac catggccgta ttccccgtta taaccgccac gataatggtc gtcatccggg
     6601 ccgactacgc agccgctgat catgccaagg gcgattgcgc ccaacaggcc ccatatccat
     6661 ttcttgtatg ctttcatgat aaaagaacgt cccccgcacg cgaaagcgaa atggggtatt
     6721 tcatcaggta ggtcaacacc ctgattgtct tcatcaacag gggcgcggag tttttgatag
     6781 ttagatggtc agggactcag tttgtttcct taaggttcaa ctcaatcgaa caacacaagc
     6841 aaacaggaaa ggctttggta tgaatccatt gactaataag cacagaataa tcatctcttc
     6901 ggtaatggcc gcgacgctag ttttgggcgc gggttgtcgc acttgcaacc gtgaatcttc
     6961 ggcgaattat gcgcccatca gcaccggcgg catgggaaac gaatatggaa ctgagtcagg
     7021 ccaatattct tcgggcggag acgtcgtgat cccgttggaa aaagagcagg cgcaagtcgg
     7081 aacgcaacgg gtggaccagg ggggagtgcg gattcgcaag ttcgtaagga cggaaaccat
     7141 caatgagccc gtgcaagtcc gcgaggaaac ggtttcggtt gaccgggttc cgggcggcgc
     7201 tcaaggcaat gccaacaccg ctttgaacac gccgttccaa agcggagaaa tcaccattcc
     7261 gttggtgaag gagcaaccgg tcgtcacgac gcaagttgtg cctgacggcg cggtagtgat
     7321 tcacaggcag gaaaccacgc aacagatgaa cgtcccggcc caggtgcgtc aggaacgcgt
     7381 cgttgccgag ccggttggca acccggcaaa cctgaacatc tcccaggacc tcaggcgggg
     7441 agaacgcgag aatgaagagc cttatgctca gggcgctcca ggggcttctt acggccagag
     7501 cagcggcaca agcggacagc cgatcacgca attgaaccaa ttgtgcaata cgtccgacac
     7561 agcctcgctc tacaactcgc aggtgaacat cagcaacgtg aaggttgacc gggtgttgaa
     7621 cgatcaactc atccaggtga gggcggacaa tggcacgaaa ttcttcgtcc acattgacca
     7681 gcccacgcag ggcctgcaca gggaccagat catcacgttg aacggcgcca tcaaacaggt
     7741 gccttcggac ccttcaacat tgggctggga ccctgccagc gcgcaggcag tccaaggcca
     7801 gcaattggtg attgattgtc cgagcgtaag tccgcaaggc tgaagcggaa aataatcgtc
     7861 aggaacgcac aaaagccggg agggattcct tccggctttt ttggcttaca aatcatctca
     7921 tcgagaaaag agacacgtag aacagattgt ttc
//
LOCUS       DASZMM010000509         5641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5448829, whole genome shotgun sequence.
ACCESSION   DASZMM010000509 DASZMM010000000
VERSION     DASZMM010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5448829"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="rny"
                     /locus_tag="VG754_12230"
     CDS             <1..1010
                     /gene="rny"
                     /locus_tag="VG754_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWF20031.1"
                     /translation="AEEKARQIISMAIQRYAGEHTFETTTATIALHGEDMKGRIIGRD
                     GRNIRAFEAATGITVLIDDTPNAVVLSGFDPVRREIAREAMTRLILDGRIHPTRIEEV
                     VTKVSQEMDETIIRLGEEAVGKTGLPPMHPEIVKLLGRLHFRHSYSQNVLDHSVEVAH
                     LMGMMAAELGIDTVLAKRAGLLHDIGKAIHHEVEGPHAIVGADFIKRYGESDAVVNGV
                     ASHHNDVPPIGPLGVLVSAADAISASRPGARSETMATYIKRVEDLEKIALSFPGVEKC
                     FAVQAGRELRVLVQPETINDEQAYALARNIAVKIEDGMQYPGQIKITVIRETRCTEVA
                     K"
     gene            complement(1024..1212)
                     /locus_tag="VG754_12235"
     CDS             complement(1024..1212)
                     /locus_tag="VG754_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20032.1"
                     /translation="MKFREFIQNRRAWLVLVLLGVGLGGFGCASTGDEPENVSSKPWN
                     SPEGWQNGNLPSMLQQPH"
     gene            complement(1214..1966)
                     /locus_tag="VG754_12240"
     CDS             complement(1214..1966)
                     /locus_tag="VG754_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20033.1"
                     /translation="MLIRIALIVAILGGIAVGVVNFVWVKDKITTTINQRDEYHKTAD
                     TETAAHRKFEKLAKDTQTKLDATNAALVSMTDERDKLQADNSDLTKKNLALTDTLEKT
                     TKERNTAQDELAAWRELGIPLTNIKATLASLKTVTEERDAIAEEKKILQKKVTALSTE
                     VDFLTGREPEVKLPAGLRGKVLVADPRYEFVVLDIGEKQGVLENGRLLINRNGKLVAK
                     VQVKSVQADRSIANVMPGWKLGEIMEGDQVLY"
     gene            2119..3408
                     /locus_tag="VG754_12245"
     CDS             2119..3408
                     /locus_tag="VG754_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20034.1"
                     /translation="MHSFNLRPRCGLKALVFAWALMQTAALLAGNVVTINSNNVLVIN
                     GKEVFPIGFTLGPPPGAKTPNGKEGLRELADAGTTFIRTGANSTNWSEETIANEQKWE
                     DAASRCGMYCWVYLHERASIRKRDSEREAMLRRVVNQFKDHPGMGIWKGADEPEWGKL
                     KTKPLVNAREIIRELDPNHPLATIEAPRGTVSSLRPYNATCDITGCDITGCDIYPISY
                     PPGKHSLLTNKEINMVGDYTRTMMEVTESKMPVWMVLQIAWSGVAKKGKTLRFPTFPE
                     ERFMTYEAIINGARGLVYFGGNVKQTLSPEDAALGWNWTFWNHVLRPVVEEIGTHSPL
                     APALIAPDSKLPVTVSEPNDIEFCVREVGGEIFLLACKRAGATAEVEFDKLPVADGTY
                     ELMYESPRSVKVSNGKFKDWFAPYEVHVYRLKRSRSS"
     gene            complement(3420..3818)
                     /locus_tag="VG754_12250"
     CDS             complement(3420..3818)
                     /locus_tag="VG754_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20035.1"
                     /translation="MASEIQTIRRPRITVLEILLLPVYLLIAPRLLGVAVLLLLLVFY
                     LLFPLHRSRRVLIIGYVLFFLCLVMPADFDVARFHGPIYGNHRSGLHFVRIKYGMLNT
                     PRCIEKYGEFVAGGCCPRGLFDPAWVLVWE"
     gene            complement(3851..4075)
                     /locus_tag="VG754_12255"
     CDS             complement(3851..4075)
                     /locus_tag="VG754_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF20036.1"
                     /translation="MAQKWLELTNPVKMPIFSTGMKKFTAIIHRGEPEEGGFWATCLE
                     VPGANGQGETRTECLDNLAAAIRDLLHCSS"
     gene            4111..5292
                     /locus_tag="VG754_12260"
     CDS             4111..5292
                     /locus_tag="VG754_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF20037.1"
                     /translation="MKYRRFGRTELSMPVISCGGMRYQFKWQDVDPKDVPAENQKNLE
                     ACIHRAIELGINHIETARGYGTSEMQLGNVLPTLPRDKIIVQTKVAPMKNPKDFLKTF
                     EKSMAYLKLDHVDLLSFHGVNNHQLLDFTLRKNGCLSIARQLQREGRVRFVGFSTHAT
                     TDIILDAINTNEFDYVNVHWYFVNDFNWPAIVAARERDMGVFIISPNDKGGKLYEPPQ
                     KLVDLCAPLSPMIFNDLYCLARPEVHTLSCGAARPTDFDEHVKALEYYDNISATIAPI
                     EKRLRAEMENVLGADWCARWFEGLPGYLEVPGEINISEILRLWTFAKSLDLIGWGKFR
                     YNLLGQADHWFPGQNAAKATATKLKKVLKNSPFAERIPAILKEAHEMLFEAPKKRLSQ
                     S"
BASE COUNT         1354 a         1584 c         1464 g         1239 t
ORIGIN      
        1 gagccgagga aaaagcgcgt cagatcatca gcatggcgat ccagcgttac gccggcgaac
       61 acactttcga gaccaccacc gcgaccatcg ctttgcacgg cgaggacatg aagggccgca
      121 tcatcgggcg cgacggacgc aacatccgcg cgttcgaagc cgcaacgggc atcacggtgt
      181 tgattgatga cacgcctaac gcggtggtgt tgtccgggtt cgatcctgtt cgccgggaaa
      241 ttgcgcgcga agcgatgacg cgattgattt tagacgggcg catccatccc acgcgcatcg
      301 aggaagtcgt caccaaggtc agccaggaaa tggacgaaac catcatccgg ctcggcgagg
      361 aagcagtggg caaaaccgga ttgccgccga tgcatcctga aattgtcaag ctgctcgggc
      421 gtttgcattt ccgccacagc tattcccaaa atgttctcga tcattccgtc gaagtcgcgc
      481 atttgatggg aatgatggcg gcagaactgg gcattgatac cgtgctggcg aaacgcgccg
      541 gactgctgca cgacatcggc aaggcgattc atcacgaagt ggaagggccg cacgcgattg
      601 tcggcgcgga tttcatcaag cgttacggcg aatcggatgc ggtggtcaac ggcgtggcat
      661 cgcatcataa cgacgttccg cccattggtc cgctgggtgt gcttgtgagc gccgccgacg
      721 ccatcagcgc gtcgcgtccg ggcgcgcgtt cggagacgat ggccacctac atcaaacgcg
      781 tggaagactt ggaaaaaatc gcgctttcat ttccgggcgt tgaaaaatgc ttcgcggtgc
      841 aggcaggccg tgaattgcgc gtccttgtcc aaccggagac gatcaatgac gaacaggctt
      901 atgcgctggc gcgcaacatc gccgtgaaaa tcgaggacgg catgcaatat cccggccaga
      961 tcaagataac cgtcatccgc gaaacccgct gcacggaagt ggcgaagtga aaatgcaaac
     1021 gcctcaatga ggctgttgca acatgctggg aagattgccg ttctgccaac cttcgggaga
     1081 attccacggt ttggaagaaa cattttccgg ctcgtcaccg gtcgaggcgc aaccgaaacc
     1141 gcccaatccg acacccaata acacgagcac aagccaagca cggcgatttt gaatgaactc
     1201 gcgaaatttc atctcagtaa agcacctggt cgccttccat gatttcaccg agtttccaac
     1261 ccggcatcac gttggcgatg gaacggtcag cttggacact cttgacctgg actttagcga
     1321 cgagtttgcc gttgcgattg atgagcaagc ggccgttttc cagcacaccc tgcttttcac
     1381 cgatgtccag caccacaaat tcataacgag gatccgcaac aagcactttg cctctcagcc
     1441 cggcaggtaa cttcacttct ggctcgcgcc ctgtcaggaa atcaacctcc gtgctcaaag
     1501 cggtaacttt cttctgtaaa atcttctttt cctcggcaat cgcatcgcgt tcttcggtca
     1561 cagtcttcaa cgaagccaaa gtcgccttga tattcgtgag cggaatgccc aattcacgcc
     1621 atgcggcgag ttcgtcctgc gcggtattgc gttccttggt ggtcttctcc aacgtatcgg
     1681 tcagcgcgag gtttttcttg gtcaaatcgg agttgtctgc ctgaagtttg tcgcgttcgt
     1741 cagtcattga gaccaaggcg gcgttcgtcg catccaattt ggtctgcgtg tccttcgcca
     1801 gtttctcaaa cttacggtgc gcggctgtct ccgtgtccgc ggttttgtga tactcatccc
     1861 gctggttgat ggtggtggta atcttatcct tgacccagac gaagttcaca actccaactg
     1921 caatcccgcc taagatggca acaattaacg cgattcgaat caacatggca caaatctttt
     1981 gagttactgg gcaagcgtag tccctgccca agccggtgtc aacgctcgtt tcacactctt
     2041 ttccagcgaa aaaaagcgcg cctcttttgc ctgcatcggc ttgcctggag gacgttcaac
     2101 acctaatctt cggctgacat gcattccttc aatttgcgcc ctcgttgcgg actcaaggcg
     2161 ctcgtgttcg cttgggcatt gatgcaaacc gctgcacttt tggccggcaa cgtcgtcacc
     2221 atcaactcca acaacgtcct cgtcatcaat ggcaaagagg tctttcctat cgggttcacc
     2281 cttggtccgc cacccggagc caagacgccc aacggcaagg aaggtttgcg ggaactcgcc
     2341 gatgccggca cgacgttcat ccgcactggc gcgaacagca cgaactggag cgaggaaact
     2401 atcgccaacg aacaaaagtg ggaggatgcc gcctcgcgtt gcgggatgta ttgctgggtt
     2461 tacttgcacg aacgggcttc gattcgcaag cgcgattcgg aacgcgaagc gatgttgcgc
     2521 cgcgtggtga atcaattcaa agaccatccc ggaatgggca tctggaaagg cgcggatgag
     2581 cccgaatggg gcaagctcaa aacgaagcct ttggtcaatg cgcgggaaat catccgcgaa
     2641 ctggacccga accatccgct tgcgaccatc gaagcgccgc gcggtaccgt ctcttcgctt
     2701 cgcccttaca acgccacttg cgacatcacc ggctgcgaca tcaccggctg cgacatctat
     2761 cccatcagct acccgccggg caagcactcg cttttgacga acaaggaaat caacatggtc
     2821 ggcgattaca cgcgcacgat gatggaagtg accgaaagca agatgccggt ctggatggtc
     2881 ctgcaaatcg cctggagcgg cgtggccaag aaaggcaaga cgttgcgctt cccgactttt
     2941 cccgaggaac gcttcatgac ctacgaagcc atcatcaatg gcgcgcgcgg gttggtttat
     3001 ttcggtggca acgtgaagca aaccctttcg ccagaagatg ccgcgctcgg ttggaactgg
     3061 actttttgga accacgtcct gcgcccggtg gtcgaggaaa tcggaacaca tagtccgctt
     3121 gcgccggcat tgatcgcgcc ggattccaaa ctgcccgtca ctgtcagcga accgaacgac
     3181 attgagtttt gcgtgcgcga agttggcggc gagattttct tgctggcgtg caaacgtgct
     3241 ggcgcaacgg ccgaagttga attcgacaaa ctgcccgttg ccgacggcac gtacgagttg
     3301 atgtacgaat cgccgcgttc ggtgaaagtt tccaacggaa aattcaaaga ttggttcgcg
     3361 ccttacgagg tgcatgtgta tcgtttgaaa cgatctcgtt catcgtagtt aaaaattgtt
     3421 cactcccata ccaacaccca agcgggatca aacaggcccc ttggacaaca accacctgcc
     3481 acgaactcac cgtatttttc tatgcatctt ggagtattca gcattccata cttaatacga
     3541 acaaaatgca aaccactgcg atgattgcca taaattggtc catgaaaacg ggcgacatca
     3601 aaatccgctg gcattacaag acagagaaaa aacagaacat atccaatgat caggacacga
     3661 cggctgcggt gtagcggaaa caataggtaa aatacaagga ggagtaaaag cacagccact
     3721 cccaaaaggc gtggtgcgat gagcaagtaa acgggaagca acaggatttc caaaaccgtt
     3781 attcgcggtc tcctgatagt ttgaatttca gaagccatgg gttccaatgc gacattcgtc
     3841 caatgccacc tcacgatgaa cagtgcaaaa ggtcccgaat cgctgcggcg agattatcca
     3901 ggcattcagt tcgagtttca ccctggccgt ttgctccagg cacttcgagg catgtcgccc
     3961 agaatccgcc ttcctccggt tcaccgcgat gaatgatggc cgtaaatttt ttcatgcctg
     4021 tgctgaatat cggcatcttg acggggttag tcaattccaa ccatttttgc gccattgcga
     4081 accttcgttt ttctggcata gttgccgcgc atgaaatatc gaagatttgg acgcacggaa
     4141 ctttccatgc ccgtcatctc ctgcggcggg atgcgttacc aattcaaatg gcaggacgtt
     4201 gaccccaagg acgtgcccgc cgaaaaccaa aagaacctcg aagcctgcat ccatcgcgcg
     4261 attgaactcg gcatcaacca tatcgaaacc gcacgcggct acggcacgtc cgaaatgcaa
     4321 ctcggcaacg tccttcccac cctgccccgc gacaaaatca tcgtgcagac caaagtcgcg
     4381 ccaatgaaaa atccgaagga ttttctcaag accttcgaaa aatccatggc ttacctgaaa
     4441 cttgaccatg tggatttgct ttcgtttcac ggcgtcaata atcatcagtt gctcgatttc
     4501 acacttcgta aaaacggctg cctttccatc gcgcgccaat tgcaacgcga aggccgcgtg
     4561 cgcttcgtcg gcttctctac ccacgcgacg accgacatca tcctcgatgc catcaacacg
     4621 aatgaatttg attacgtgaa tgtgcattgg tatttcgtca atgacttcaa ctggccggcc
     4681 atcgttgccg cgcgcgaacg ggacatgggc gtgttcatca tcagcccgaa cgacaagggc
     4741 ggaaaacttt acgaaccgcc gcaaaagctc gttgaccttt gcgcgccatt gtcgccgatg
     4801 attttcaacg acctttactg cctcgcgcgt ccggaagtcc atacgctcag ttgcggcgcg
     4861 gcgcggccca ctgatttcga tgagcacgtc aaagcgctcg aatattacga caacatttcc
     4921 gcaaccatcg cgcccattga gaaacggttg cgcgccgaaa tggaaaacgt cctcggcgcg
     4981 gactggtgcg cgcgctggtt tgaaggattg cccggctatc tcgaagttcc cggtgagatc
     5041 aacatttccg aaatattgcg gctttggact tttgcaaaat ccctcgacct aatcggctgg
     5101 ggcaagtttc gctacaacct gctcggtcag gctgaccatt ggtttcccgg ccagaacgcc
     5161 gccaaggcaa ccgcgacaaa actcaaaaaa gttttgaaga atagcccctt cgccgaacgc
     5221 atccctgcga tcttgaagga agcgcacgaa atgcttttcg aagcaccgaa gaaacggttg
     5281 agccagagtt gaatatccaa ccggaccgct cttgcccgca tggtcaagcc agccttgact
     5341 cagcaaaaaa tagcttgact agccttgatt ggaatttttt gtaaatttgc tgatgttgct
     5401 gataacaatt ggcttgcgct gttttgcatg gcttgactca gtaacgttaa aggcttgact
     5461 ggtaacgtta cagtcaaggc ttgattcaga gacggcaact gtgaacctca aactttaaac
     5521 cctaaacttc ccgcagggga ggggtgccgt ttttagcggg ataatgcggg cctaagcggg
     5581 acgatgcggg atttcaacca cttacaacgt gggacgcacg tagctgaaaa cgtgagtttt
     5641 c
//
LOCUS       DASZMM010000510         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4891496, whole genome shotgun sequence.
ACCESSION   DASZMM010000510 DASZMM010000000
VERSION     DASZMM010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4891496"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VG754_12265"
     CDS             <1..324
                     /locus_tag="VG754_12265"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWF20038.1"
                     /translation="YAIMYFGFGFTWSAMQYVHHYGTERHVTRGARNLWLWEPIDRLW
                     LNHNWHLVHHEHPTVPWVHLPGLGSTTQTDKRGFILWSYLKMWRGPRKAKEHVENQYA
                     GRVIR"
     gene            473..976
                     /locus_tag="VG754_12270"
     CDS             473..976
                     /locus_tag="VG754_12270"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWF20039.1"
                     /translation="MDNWAGFQPTPWAWVYESNFLITGFIPWLIVSRMELRRYAIGFA
                     LLSALSFLIFAVFPVASPRPINQEENATLIFITHVDGPLNAFPSLHAASLVYTIALAR
                     RLFGRQLKLSVMAGLLVWASLILFATLATKQHYAVDLVAGGLIGWLADWFAWHKADSG
                     AMASAKT"
     gene            1312..1653
                     /locus_tag="VG754_12275"
     CDS             1312..1653
                     /locus_tag="VG754_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20040.1"
                     /translation="MAPKPGYKRGSLLQSAHTNPGNDIAEKSFGIGQGQRDLYVSASF
                     AQANQKHPLFFILQEPFGNNLRVSGQRTKALHGNEFDWATHRFAYGDQSRPIRSAVLV
                     FAIKGAGNDAN"
     gene            complement(1893..2504)
                     /locus_tag="VG754_12280"
     CDS             complement(1893..2504)
                     /locus_tag="VG754_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF20041.1"
                     /translation="MKKILTIIPTFIFLVWAAQAQVNAPPLDPAVDKAITRLREGLVD
                     SFNRGDIDKLLTFLDTNAVVTWQNGEVCEGTAAVKAYYDRMMKGDHPVVSKVTSNPKV
                     LGRLYQGDWAVSWGELNDTFVLTDGRELPLNSKFTATIARRGDLWLVTAFHVSVNAFN
                     NPITALTVKRVSIIVGIAGLVAGLLAGMILMRLLRGAKSSNAA"
     gene            complement(2501..3598)
                     /locus_tag="VG754_12285"
     CDS             complement(2501..3598)
                     /locus_tag="VG754_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWF20042.1"
                     /translation="MFCPFLWCESHCKLNRAKVSKYLTPHIALTDSKSCAGPERGKSG
                     FPQVPVSWFYLCASAELNRGPVSVDLCGFSFVGYRTQSGRAIVLSGRCAHLGAKLANG
                     TVNGERLVCPLHGWEYGPDGVCERIPAVDDIPCFARQLSFPVEERGGHVFFFNRQRAR
                     FALPFFEGISPDQLLSTEAFDLVAETPWYFAGANGFDIQHFRMAHDRTLIGQPEVTTP
                     SPFARRVVANFEISGNSYQDRLTRCVAGARVTMNVTVWCGSLIMVRAEFARTTSFGIF
                     NVLPIDANHTRGRVIVWVRRSKSAIGRLLFDPLNAAIRRMFIRTFLRSDLPRIAGLRY
                     QPGNLIIADKVLAEYFNWLEMISEPSPTENL"
     gene            complement(3608..>3947)
                     /locus_tag="VG754_12290"
     CDS             complement(3608..>3947)
                     /locus_tag="VG754_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20043.1"
                     /translation="MTCSISRLIKCNGAAGSVAGTCFVNEVPVEGESFPTIFVYYHAA
                     DFSFLFFLRVALMPPNYNSRVATEVAVATSQMAEFWGCWACFRRKEKAKRLRMAAAKH
                     TTEPGRWARS"
BASE COUNT          928 a         1095 c         1019 g          905 t
ORIGIN      
        1 tacgcgatca tgtatttcgg ttttggtttt acatggtctg ccatgcagta tgttcatcat
       61 tacggtaccg aacggcatgt gacgcgcggc gcacggaatc tctggctttg ggaacccatt
      121 gatcgccttt ggctgaatca caactggcat cttgtgcatc atgagcatcc aaccgtgccc
      181 tgggttcatc tacccgggct tggcagcacg acgcagactg acaagcgggg cttcatcctc
      241 tggtcttatc ttaaaatgtg gcgcggcccg cggaaggcaa aggaacacgt cgaaaatcaa
      301 tatgccggac gtgtcatccg ttgatctggg tgcatgcctg tggcggcgca ttactgcgct
      361 ttggcccgca aaactgtggt taacggccat cttgggcatt tgcttttggg tttgttatct
      421 tttcctttca cggcacgcgt tggttcaaat tcactcgttg ccgatcacat tcatggacaa
      481 ctgggcaggc tttcagccga cgccttgggc atgggtatat gagtcgaatt tcctcatcac
      541 tggcttcatt ccctggttga tcgtttcgcg aatggaactg cggcgctacg cgattggatt
      601 cgcgctgctt tcagctttga gctttttgat ttttgccgtt ttcccggttg cctcgccacg
      661 accaatcaat caggaagaga atgcaactct gattttcatc acgcatgttg atggaccgtt
      721 gaatgctttc ccttcgctgc atgccgcatc tctcgtttat acaattgccc ttgcacgccg
      781 attatttgga cgtcaattga agttgagcgt gatggccggc ttgctggttt gggcatccct
      841 gattcttttt gccactttgg ccaccaaaca gcactacgcc gtggacttgg tggcgggagg
      901 gttgatcggg tggttggcgg actggtttgc ctggcacaag gcagactccg gggccatggc
      961 ttcggccaaa acctgacgca aaagcggcac cacatcccag gtgggctgaa ggtaatggtc
     1021 cgctccccaa acaggaagca ctgtgccggg aagttgcgca acatctgcca ggaggaatcc
     1081 gccaccgtcg ttcggcccca atggcgcgag tcgttcatac gcacgtggtg cccagggatg
     1141 agcaagatgt cggcgcaatg gaaatcctaa aatatggatc agtcgcaaac tcggtggcaa
     1201 cgccggccaa acggccaacg gcccatcggc ttccggcctc aattcttcga ctaccgaata
     1261 acgttgccct cgcaggcgga gcaaaagtct tacgcccagc cggcgtaatc gttggcgccg
     1321 aagccaggct acaagcgggg ttccttgctg cagtccgctc atactaatcc aggcaacgac
     1381 attgccgaaa agagcttcgg cattgggcaa ggccagcgcg acctttacgt cagcgcttcc
     1441 tttgctcagg ctaatcaaaa gcacccgctg ttttttattc tgcaagagcc atttggaaat
     1501 aacctccgcg tttcgggcca gcgaaccaaa gctctccacg ggaacgagtt tgactgggca
     1561 acccatcgct tcgcctatgg cgaccaatcg cgccccatcc gctccgctgt gcttgtgttc
     1621 gcgataaaag gcgccgggaa tgatgccaat tagggtcggc tgaacggcga ggagcggatc
     1681 aatggtttgg actcgcttaa aaaatgtccg gtttgtttca ttacgaagga cccggtcata
     1741 cagcactgcg gtggcaaatt cgatgccctc ctcttgagca agttctttca accgtaaggg
     1801 cgagcaattg gctaaagagg tacattggtt ccatgccgcc gcccgtgcga gcaaatcatt
     1861 gtcttcagcg gaaatttcgc ggggacgact cgtcatgccg cgttgctgct ttttgcgccg
     1921 cgcagcaaac gcatgagaat cattccagcg agcaagccag ccactaatcc cgcaatgccg
     1981 acaataatcg aaacccgttt cacggtaagg gccgtgattg gattattgaa tgcgttaacc
     2041 gaaacatgga acgccgtaac cagccacaga tcgccccgtc gggcaatcgt tgccgtgaat
     2101 ttgctgttca atggcaattc gcgcccgtcg gtaagcacaa atgtgtcgtt caattctccc
     2161 cacgacacgg cccaatctcc ttggtaaagg cggccgagaa cttttggatt ggaggtgact
     2221 ttgcttacca ccggatgatc gcctttcatc atgcgatcat aatacgcctt tacggctgct
     2281 gttccttcgc aaacttctcc attttgccag gtcaccaccg cgtttgtatc gagaaacgta
     2341 agtaatttgt cgatgtcgcc acgattaaaa ctgtccacca atccttctct caagcgggtg
     2401 attgccttgt caacggcggg atcgagcggc ggtgcgttaa cctgagcctg ggctgcccac
     2461 acaagaaaaa tgaaagttgg aatgattgtg aggatttttt tcataagttt tcagttggtg
     2521 aaggctccga gatcatttcc agccagttga aatattccgc caggactttg tcggcgatga
     2581 taaggtttcc aggttgatag cgcaaaccgg cgatccgggg caagtcagaa cggagaaatg
     2641 tccgtatgaa catccggcga atcgccgcat tcagtggatc gaacaacagg cgtccgatgg
     2701 cgcttttgct gcgccgcacc catacaatta cccggccacg ggtatggttg gcatcgatgg
     2761 gtagcacgtt gaagatgcca aaacttgtag tgcgagcgaa ttcagcgcga accatgatca
     2821 aacttccgca ccacaccgtg acattcattg tgacccgcgc accagcgacg caacgcgtca
     2881 atcgatcctg atacgaattg ccagaaatct cgaagttcgc caccacccgc ctcgcaaagg
     2941 gcgagggggt cgttacttcg ggttggccaa tgagggtgcg atcatgtgcc atgcggaaat
     3001 gttggatgtc aaaaccgttc gcaccggcaa aataccaggg tgtttctgcg accagatcaa
     3061 atgcctcggt ggacaacagt tgatcggggg aaataccttc aaaaaatgga agcgcaaacc
     3121 gggcgcgctg acgattgaag aagaaaacat ggccaccccg ttcttccacc ggaaaactca
     3181 attgccgcgc aaaacatgga atgtcgtcca ccgccggaat tctttcacaa acgccatccg
     3241 ggccgtattc ccagccgtgg agcgggcaaa ccagacgctc gccgttcacc gttccattgg
     3301 ccagtttcgc gccaagatgc gcgcaccgac ccgacaaaac tatcgcacgc cccgattggg
     3361 ttcggtagcc gacaaaagaa aagccgcaaa gatccacact gaccgggccg cgattcaatt
     3421 cggcactcgc gcacaaataa aaccacgaaa cgggaacctg ggggaatccc gatttgccgc
     3481 gctccggtcc ggcgcacgat ttggaatcag tcaaggcgat atgtggagtc aggtatttag
     3541 aaacctttgc ccgattcaat ttacagtggc tctcacacca taagaaagga caaaacaaca
     3601 gcgctaatta actccgtgcc caccttcctg gttccgtcgt gtgtttcgct gccgccatcc
     3661 ttaagcgctt ggccttctcc ttccgccgga agcatgccca acagccccag aattccgcca
     3721 tctgcgaagt ggcaactgcg acctcagttg caaccctgct attataattg ggcggcatca
     3781 gtgccaccct gagaaaaaac aaaaatgaaa aatcagctgc atgataataa acaaaaatag
     3841 tcggaaaact ctccccttcc acgggaactt cattaacaaa gcaggtgccg gcaacactcc
     3901 cggcagcacc gttacattta atcagcctgg atatgctaca agtcatc
//
LOCUS       DASZMM010000511         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2965861, whole genome shotgun sequence.
ACCESSION   DASZMM010000511 DASZMM010000000
VERSION     DASZMM010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965861"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..607)
                     /locus_tag="VG754_12295"
     CDS             complement(383..607)
                     /locus_tag="VG754_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20044.1"
                     /translation="MSPELEKLLEALYEKFNCPPEEKSHRVATFERLLQDALARRPGA
                     SREELLDALRDRYREFLRSRRKPPTIPPKA"
     gene            complement(597..983)
                     /locus_tag="VG754_12300"
     CDS             complement(597..983)
                     /locus_tag="VG754_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20045.1"
                     /translation="MSTAALTTPMDEPVDLAGGVSKRIMRIFRAQVEDWCDVCRQVSG
                     WEERHFIDALTPEKFKEHAHILDELESVGRWLSLATQSEDFPDRSTADLVSMTLQDLK
                     DRRALWHGKLKPQEREKILRDIFNES"
     gene            complement(1107..>2146)
                     /locus_tag="VG754_12305"
     CDS             complement(1107..>2146)
                     /locus_tag="VG754_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWF20046.1"
                     /translation="TTTTSLLTWVFEHNGLNPSYLIGGIPNNLSQGARFTDSEWFIIE
                     GDEYDTAFFDKRSKFVHYLPEIAIINNLEFDHADIFENLDAIKKSFSHFIRLVPRNGL
                     LLGNGDDANLASLLNVTHCPVKKFGLGGDYAVYAFNIRYGPTATEFEIPSFKFHLNLV
                     GELNVRNALAVIACAKHCGLSNKQIQAAFDTFKGIKRRMEVRGIAGGVTVIDDFGHHP
                     TAIRETLRALRIKYPQQQLWAIFEPRSNTTRRNVFQEELASAFADADAVVVSEVARLE
                     QIAVAERLNPEKLMQDLKASGKKAAYLADVDAIVAHVSGHIQGGDIVCVFSNGGFGGI
                     HGKLLERLGRR"
BASE COUNT          476 a          647 c          545 g          478 t
ORIGIN      
        1 cgacgtaatc tccgttgcag atttccggtg ctgcccacac tgaattgagg ctcaagaagg
       61 cgaaggccat tattattcga ttcataaacg tgcccgcatc aaattgtgga gctaattcca
      121 ctcacgcaca atggtaacta cctgcctcgc gcgggatgaa tcgaaaaaaa tgacgcgaac
      181 atcaaccgga acgatgccgc gcaaaaattg aagccgtatt ccagcgctcc ctggcgcttg
      241 ctccgctttc ccgaagcatt gcaacgtccc actggcaatc agtttcgccg tagcgcaatg
      301 aattcctgac gagactcggc gcgtaattct catctcctcc caacttcaca tgagttctgg
      361 cgacggcgct tatcgggcct gctcacgcct tcggcggaat tgtcggcggt ttgcggcgag
      421 agcgaagaaa ctcccggtag cggtctcgaa gcgcgtctaa caactcttcc cggctcgctc
      481 ccggacgccg cgcgagagcg tcttgcaaca aacgctcaaa ggtcgcgacg cgatggcttt
      541 tttcctcggg agggcaattg aacttttcgt aaagcgcttc cagcaacttt tccagttcag
      601 gactcattga agatatcgcg cagaattttt tccctctcct gcggcttcaa tttgccgtgc
      661 caaagcgccc gacgatcttt caaatcctga agtgtcatgg aaaccaggtc cgccgtggat
      721 cggtctggaa aatcctcgct ttgggtggcg agcgaaagcc agcggcctac gctctccaat
      781 tcgtcgagaa tatgcgcgtg ctccttgaat ttctccggcg taagagcatc gataaaatga
      841 cgctcctccc atccgctcac ctggcggcag acatcgcacc aatcctccac ttgcgccctg
      901 aaaatccgca tgatccgctt ggaaacgcca ccggctaaat ccaccggctc gtccatgggg
      961 gttgtcaacg cggctgtgct cattgcctaa aatatgagcc gaatacggcg gattggcaag
     1021 attgcttata aatcgcggac atccggtgac acttttcccg cacgagccga tcgttggtta
     1081 tgtttgcggg caaattgcct cgagacttac ctcctcccca accgctccag caacttgccg
     1141 tggattccgc caaagccgcc gttgctgaac acgcacacaa tatcaccgcc ctgtatatgg
     1201 ccggaaacgt gcgccacaat ggcgtccaca tccgccaaat acgccgcttt ttttccggaa
     1261 gccttcaaat cctgcatcag cttttccggg ttcaaccgtt cggccacggc gatctgttcc
     1321 aaccgcgcca cttctgaaac caccaccgcg tcggcatcgg caaaggccga agccagttcc
     1381 tcctggaaca cattccgccg cgtggtgttg ctgcgcggct caaaaatcgc ccaaagctgt
     1441 tgctgcggat acttgatgcg caacgcgcgc agcgtctccc ggatcgccgt cggatgatgc
     1501 ccaaaatcgt cgatcaccgt caccccgccc gcgatgccgc gcacctccat gcggcgtttg
     1561 atgcccttga aggtgtcaaa cgcggcttga atttgtttgt tcgaaaggcc gcaatgtttt
     1621 gcgcacgcaa tcacggccag cgcattgcgc acgttcaatt caccgaccag gttcaggtga
     1681 aatttgaagc tcggaatttc aaactcggtt gccgtggggc cgtaacgaat gttgaacgca
     1741 tacaccgcgt aatcgccgcc caacccgaac ttcttcacgg ggcaatgcgt cacgttcagc
     1801 agcgacgcga gattcgcatc atcgccgttg ccgagcagca agccattgcg cggcaccaac
     1861 cggatgaaat gcgagaacga tttcttgatc gcgtcgaggt tctcaaaaat atccgcgtga
     1921 tcgaattcca ggttgttgat gatggcaatc tcgggcaggt aatgaacgaa cttgctgcgc
     1981 ttgtcgaaaa acgcggtgtc atactcatca ccctcgatga tgaaccattc gctgtccgtg
     2041 aagcgcgcgc cttgcgaaag attgttgggg atgccgccga tgagatagct cgggttcagc
     2101 ccgttatgtt cgaagaccca tgtgagcaag gacgtcgtag tggtct
//
LOCUS       DASZMM010000512         5497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2693143, whole genome shotgun sequence.
ACCESSION   DASZMM010000512 DASZMM010000000
VERSION     DASZMM010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693143"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..594)
                     /locus_tag="VG754_12310"
     CDS             complement(61..594)
                     /locus_tag="VG754_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF20047.1"
                     /translation="MDEHERSKAFEQIVLPHLDAAYNLARWLAGNDHDAQDVAQEASL
                     RAFRFFGSFRGDNARAWLLAIVRNTFYTWLRKNRPPEKTIEIDDETFAIEDASPNAEP
                     VNPQFADADAVRNAIAELPVEFREIIILREMEDLSYKEIADLAEVPIGTVMSRLARAR
                     KLLQKRLAAAFNPGDQR"
     gene            871..1884
                     /locus_tag="VG754_12315"
     CDS             871..1884
                     /locus_tag="VG754_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF20048.1"
                     /translation="MKNKPNRITEVRRLSAEAEHIDHDSSHDGVDRRGFLKCMAWAGT
                     GMLWTISGGVLGSQILRPLNSAAADGGAAMMGDFSFVQISDSHIGFNKPANTDVIGTL
                     KQAIAKINALPAAPDFILHTGDLSHLSEPEEFDTLDQLLKTARAGKVFYVPGEHDVIS
                     DTGKEYRARYGKGTKGSGWFSFDQRGVHFIGLVNVMNIAEGALGNLGDEQLAWLKDDV
                     SGLSSSTPIVVFAHVPLWSIYPKWGWGTQDAEQALGFLRRFGSVTVLNGHIHQTFKKV
                     EGNVTFHTATSTAFPQPAPGTAPKPGPMKVSPEILPTVLGITDVNYVESNHSLAIVDA
                     KLA"
     gene            1897..2319
                     /locus_tag="VG754_12320"
     CDS             1897..2319
                     /locus_tag="VG754_12320"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HWF20049.1"
                     /translation="MNQTLWARMHGGMTHFPIALLLVSVFFDHIGFWWNDEVRRREFR
                     SIGFYTLLMAAAASPLAVLSGIALSRGKLMGSGMLALHHYFVWPAFGFLLGLAVWRLV
                     VREHASPRARGCYLALASLTLVSMMTAGYWGGEMIIGK"
     gene            2326..2709
                     /locus_tag="VG754_12325"
     CDS             2326..2709
                     /locus_tag="VG754_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF20050.1"
                     /translation="MKLSLELKGTILGILTGCSALGIAFAASELFQPKATQRADANHA
                     LEVPPEGTLANQGYKLFLLNCAHCHGDDARGDEGPDLHDVTKSDQRIANLIQNGVKDQ
                     MPKFSAKLTDADVQALIAFIRALRN"
     gene            2725..2853
                     /locus_tag="VG754_12330"
     CDS             2725..2853
                     /locus_tag="VG754_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20051.1"
                     /translation="MSVIADGVLSLVSKGEIVMRTITALAAGSLIATTNWNSTGRN"
     gene            complement(2869..4776)
                     /locus_tag="VG754_12335"
     CDS             complement(2869..4776)
                     /locus_tag="VG754_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWF20052.1"
                     /translation="MSAILAATDLTVRYNERTILASATLGIDESDRIGLVGRNGCGKT
                     TFLKILAGLQSPDAGTVTARRDLVVSYLPQDFTLDRSLSVRDNVRHGARHVLDLIAEF
                     ESLPAESKRHETLEHRIATLDGWSLDQRIETAMSHLDCPPGDRSIDSLSGGEQRRVAL
                     ARAIVSRPELLILDEPTNHLDPESIEWVAEFLENFRGAFLVVTHDRYFLDRVVNQIVE
                     LSDGRFFNHDGNYTDYLLDKAERQAADATVEHKRQMFLKKELAWVRQGPRAQRSKQKN
                     RFERYYETAAQEVAPVEEDMELVIPPPPPLGNRVVELSGVGMELGGRALFSGFDFTFE
                     NGRRVGICGRNGMGKTTLLKIILGQLAPTQGTVKTGQLTRFNYVDQSRLQLNEERTVL
                     EEAADGTEFVQWGESKISLRSYLKRFLFADERITTRVKHLSGGERSRLLLARILKCGG
                     NFLMLDEPTNDLDLPSLRVLEEALIAFPGVVCVVSHDRYFLDRVCTDILAFEGDGRIH
                     HSVGDYDYYLEKRRKRVEGLKQQTAESAPDRTAAASSRTTGTASKPAKPAKFSFKQAR
                     ELESIEPQILATEEEVARIEQLFASPDFHRTHATRTNELLAELAAAKTKAVQLYARWE
                     ELEALKSLPQE"
     gene            complement(5051..>5497)
                     /locus_tag="VG754_12340"
     CDS             complement(5051..>5497)
                     /locus_tag="VG754_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HWF20053.1"
                     /translation="AAGTDKIWTGLPALTILLLGGFTTNFAWCIFLNIKNKTGGQYFS
                     SEIRGAPPEPAPMLANYLFCALAGTCWYFQFFFYTMGATQMGKYDFASWTLHMASIII
                     FSTIWGWILREWKGAGKKAHLLIAGGIATLILSTIVIGWGAYLKSH"
BASE COUNT         1196 a         1563 c         1517 g         1221 t
ORIGIN      
        1 ccgaagcttc caactcgcca tcggcgtagg gatcgataag tggtttggct tgttcgcaat
       61 tcatcgctgg tctccgggat tgaatgcggc ggcgagtcgc ttttgcaaca gcttgcgcgc
      121 gcgcgccagg cgggacatga cggtgccgat gggcacttcg gccagatcgg caatttcttt
      181 gtaactcaaa tcttccatct cgcgtaaaat gatgatctct cgaaattcca ccggcagttc
      241 ggcgatggca ttgcgcaccg cgtcagcgtc cgcaaactgc gggttgaccg gctccgcgtt
      301 cggcgaagca tcttcgatgg cgaaagtttc atcatcaatt tcaatcgttt tttccggcgg
      361 acgattcttg cgcagccacg tgtaaaaagt gtttcgcacg atggcgagca gccacgcccg
      421 cgcattatcg ccgcgaaaac ttccaaaaaa cctgaacgcg cgcaaactgg cttcctgcgc
      481 cacatcctgc gcgtcgtgat cgttgccggc gagccaccgc gcgagattgt atgccgcgtc
      541 cagatgcggc agcacaatct gctcgaatgc ctttgatcgc tcgtgctcgt ccactcagac
      601 tatttgtgac tcgtcccgtc atttagcaag accaatatcc atctcatttt attcccggaa
      661 ttatttttta cgcgcgggaa taaaacactc aatggagccg gtctggatta atcagcaaag
      721 gcctcatgaa aacgaccatc aattgtgcgg catggatttc caagaagttt acgccggaga
      781 taatatttgc atttccggga ataaatcccg gcactgcgcg gtatgcatct gtcggtgacc
      841 gcaaaacaaa atcacacaaa cgtaaatcgc atgaaaaata aaccaaatcg aatcacggaa
      901 gttcgccgtc tgtcggcgga agctgaacac attgatcacg actccagcca tgacggcgtt
      961 gaccggcgcg gcttcttgaa atgcatggcc tgggccggca ctggaatgtt gtggacgatc
     1021 agcggcggtg tgctgggttc gcaaatcttg agacccctca attcggctgc cgctgatggt
     1081 ggcgcagcga tgatgggtga cttcagcttc gtgcaaatca gcgacagcca cattggcttc
     1141 aacaaaccag ccaacacgga cgtcatcggt accctcaagc aggcgattgc caagatcaat
     1201 gccttgcctg ccgcgcctga tttcatcttg cacaccggcg atttgtcgca tctttcagaa
     1261 ccggaggaat tcgacacgct ggatcaactt ctcaagaccg ccagggccgg aaaggttttt
     1321 tatgttcccg gcgagcatga tgtgatcagc gacaccggca aggaatatcg cgcccgttac
     1381 ggcaagggga ccaagggcag cggttggttc agcttcgacc agcgcggcgt ccacttcatc
     1441 ggcctcgtca acgtgatgaa catcgccgaa ggcgcgttgg gcaatctggg cgacgaacaa
     1501 ctcgcctggt tgaaagatga tgtgagcggc ctctccagca gcacgccgat cgtggtgttt
     1561 gcgcacgtgc cgttgtggtc catttatccc aaatgggggt ggggcacgca ggacgccgaa
     1621 caggcgcttg gttttctgag acgctttggc tccgtcaccg tgctcaacgg ccacatccat
     1681 cagacattca aaaaagttga aggcaacgtc acgtttcata ccgcgacttc cacggctttt
     1741 ccgcaacccg cgccgggcac ggccccgaaa cccggcccga tgaaagtgtc gccggaaatc
     1801 cttcccaccg ttcttggcat caccgatgtg aattacgtcg agagcaacca cagcctcgcc
     1861 atcgtggatg caaaacttgc gtaaatcagg ttgtttatga atcaaaccct ttgggcgcgc
     1921 atgcacggcg ggatgacgca ttttcccatc gcactcctgt tggtctcggt ctttttcgac
     1981 cacatcgggt tctggtggaa tgatgaagtg cgtcgccgtg aattccgctc cattgggttc
     2041 tatacattgc tcatggcagc ggcggcttct ccattggccg ttctttccgg aatcgcgttg
     2101 agccggggaa aactcatggg cagcggaatg ctggccctcc atcactattt cgtctggccc
     2161 gccttcggtt tcctgcttgg attggctgtc tggcgtcttg tggttcgcga acacgcgtca
     2221 ccgcgcgcgc gtggctgtta tctcgcgctg gcgagcctca cgttggtttc catgatgacc
     2281 gcgggttatt ggggtggaga aatgatcatc ggcaaataga agctcatgaa attatctctc
     2341 gaactcaagg gaacaattct gggaattctc acaggttgct cggcgctcgg aatcgctttt
     2401 gccgccagcg aattgtttca accaaaggcg actcagcgag ccgacgcaaa tcatgcgttg
     2461 gaagttcctc ccgaaggcac cctggcgaat caaggctata aattgtttct gctaaattgc
     2521 gcgcattgcc atggcgatga cgcgcgcggc gatgaagggc cggacttgca tgacgtgaca
     2581 aaatcggatc aacgcatcgc caacctcatc caaaacggcg tcaaggatca aatgccaaag
     2641 ttcagcgcaa aattgaccga tgccgacgtg caggcgttga tagcgtttat acgggcatta
     2701 agaaattaac caactctgct ggtaatgtcc gttattgcgg atggcgttct ttcgctcgtg
     2761 agcaagggtg aaatcgtcat gcgaacgata accgcgctgg ccgcggggag cttgattgca
     2821 accaccaact ggaactcaac tggtcgaaac taaagtcccg caatgattct actcctgtgg
     2881 caggctcttg agcgcctcca gttcctccca tcgcgcgtaa agttggaccg cctttgtttt
     2941 ggcagcagcc aattccgcga gcagttcgtt ggtcctggtt gcgtgtgtgc gatggaaatc
     3001 cggcgatgcg aacaactgct cgatccgtgc cacttcctct tcagtggcga gaatttgtgg
     3061 ctcgatggat tccaattcgc gcgcctgctt gaacgaaaat tttgcaggct tcgccggctt
     3121 cgacgcggtc ccggtggttc gcgaagaggc agccgccgtc cggtcgggcg ccgattccgc
     3181 cgtctgctgc ttcaggcctt caacccgttt tcgccttttc tcgagataat aatcgtagtc
     3241 tccgacacta tggtggatcc gcccatctcc ctcgaacgcc aaaatatcgg tgcagacacg
     3301 gtcaagaaag tagcggtcgt ggctgacgac gcataccact ccaggaaacg cgatcaaggc
     3361 ctcctcgagc acgcgcaatg agggcaggtc aaggtcattg gtcggttcat ccagcatgag
     3421 aaaatttccg ccgcacttga gaatgcgcgc caacaacaaa cggctgcgct cgccgcccga
     3481 gaggtgtttg acgcgtgtcg tgatccgttc gtcggcgaaa agaaagcgct tgagataact
     3541 gcgcagtgag atttttgact cgccccattg cacgaattcg gtgccgtcgg cggcttcctc
     3601 cagcacggtc cgttcctcgt tcaattgcag ccgcgattgg tccacataat tgaaccgggt
     3661 gagctggccg gtctttaccg ttccctgcgt tggcgcgagc tggccgagga tgatcttcaa
     3721 caaagtcgtc ttgcccatgc cgttgcgtcc acaaatcccg acgcgccggc cattctcaaa
     3781 cgtgaagtca aatccactga aaagcgcgcg cccgccaagc tccatcccga cgcccgataa
     3841 ttccacgacc cggtttccca gcggcggcgg cgggggaatc accagttcca tgtcctcttc
     3901 cacaggggcg acttcctggg ccgcggtttc gtaatagcgc tcgaagcggt tcttctgctt
     3961 gctgcgctgg gcccgcggtc cctggcgcac ccaggcaagt tcctttttca ggaacatctg
     4021 ccgcttgtgt tcaacggtgg catcagccgc ctgccgctcg gccttgtcca gcaggtaatc
     4081 ggtgtaattg ccatcgtggt tgaaaaaccg tccgtcgctc agttccacaa tctggttgac
     4141 cacccggtcg agaaaataac ggtcgtgcgt gacgacgagg aacgcgcccc tgaagttttc
     4201 caaaaattcc gcgacccact caatggactc cgggtcgaga tggttcgtgg gttcgtcgag
     4261 gatgagcagc tctggccgcg aaacgatggc ccgggccagc gccacgcgcc gctgctcccc
     4321 gccggagagg gaatcaatgc tgcggtcgcc cggcggacaa tccagatgcg acatggcggt
     4381 ttcaatccgt tgatcgagcg accagccatc gagcgtcgcg atgcggtgtt ccagtgtttc
     4441 gtgccgtttc gattccgcgg gcaacgattc aaattcggcg atcaaatcca gcacatgccg
     4501 cgctccatgg cgaacgttgt cgcgcacgct caacgaccga tccagcgtga aatcctgcgg
     4561 cagatagctc acgaccaggt cgcgccgggc ggtgaccgtg ccggcatcgg gtgattgcag
     4621 gcccgccaga atcttcagga atgtggtctt gccgcagcca ttccggccaa caagtccgat
     4681 gcggtcgctc tcatcaatgc cgagggtcgc agaagccagg atggtccgct cattatagcg
     4741 gacagtcaaa tcggtagcgg caagaatcgc ggacattcca atgctaaaat aagggaaatc
     4801 ggcggcggag tcgaaatcaa agttgtcgcg gtcccaaagt gagttggcac acgacgcaca
     4861 atcaagcgag ccaaaagatg tggcggatga cgggaatcga atcctcgttt caggcttggg
     4921 agtgtccctg aggaaccgta ttcagggggc tttctgccat tcgttttgga agaatttcct
     4981 cttaaagaac tgccaaattt cagttcagga ttttattctt cacaatttcg ttccacaggt
     5041 gtcacgcggt ttaatggctt ttcaaataag cgccccagcc gatcacgatg gtggaaagaa
     5101 tcagcgtggc aatgcccccg gcaatgagca aatgcgcctt tttgccggcg cccttccatt
     5161 cacgcaaaat ccagccccaa atcgtgctga aaatgatgat gctggccatg tgcagcgtcc
     5221 acgatgcaaa atcatatttg cccatctgcg tcgcgcccat cgtgtaaaag aaaaactgga
     5281 aataccagca cgtccctgcc agcgcgcaga agagataatt ggccagcatc ggcgcgggtt
     5341 ccggcggcgc gccgcgaatt tcagaactga aatattggcc gccggttttg tttttgatgt
     5401 tcagaaaaat gcaccaggca aaattggtcg tgaaaccgcc gagcaggaga atggtcagcg
     5461 cgggcaggcc ggtccagatt ttgtcggtgc cggcggc
//
LOCUS       DASZMM010000513         4907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3764761, whole genome shotgun sequence.
ACCESSION   DASZMM010000513 DASZMM010000000
VERSION     DASZMM010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3764761"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3001
                     /locus_tag="VG754_12345"
     CDS             <1..3001
                     /locus_tag="VG754_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWF20054.1"
                     /translation="DSSSTQPGSGEADLAQSKDSLQFQSWLKNQGLPRVTVDAIQQTS
                     DGYLWIAAGGSIARFDGVRFVPLNAHVSETNFSTQTIFGDSQGALWVAVSNGLQRWEN
                     GHATMLTLNDGLPNTHITALNEDGQGNIWIGTTSGLAIWNNGRLVPLADAKRIRGRAI
                     TAIFRDRKNSMWLVVQGTEVFQFDHDHFSPLTTDSAKDWMTDIHALLVDAMGRVWLAT
                     GEDAVLCRDKDGWRHYRIPKRTTGSRVNAIAEEPDGTVWAGGAGGLFLFSNGKFSAVS
                     PSSRLAGNTVRSLFVAHDGALWAGTDEGLNRLQHKCLFALGQGDGLGFGPVQGLAQVS
                     PGVVWAARTGDGIYRWDGRSFNRLRAAGLSAHNSQANALLLARDGACWVASYGGLLRY
                     KDPVAAADEVRWFELPGEDIISLAEDQDGSLWAGTRAGKLWQLREGKWLEQNSFSISS
                     SINVILPAPDGSVWIGTGGAGLIRMDHNVATFLGPNRGLPNKVVRALYLDAQGVLWIG
                     TAAGLSCMRSGKISNFTAGDGLPENPVLQILEDSTGRLWLGTSQGIACVHKDQLENFA
                     AGKIEALHPKIFNHADGMTSEECTGGFCCIALSQSGLLWFPTTKGVTVIAPQSWPVEK
                     PLPGTVIEEILLDGVPESASSDNAKLTIPAGKHRLEFVYTGLRFDAPGTIRFRYKLEG
                     WDADWIDAGTSRSAVYNFVPPGQYRFRVIASNSDGIWANRGAEIRLMFARYFWQSWWF
                     IGLAGITLLSAVGGTVRLIERQKTKSRLKRLEQERALERERTRIAQDLHDEMGAKLCR
                     ISFLSEHARRDSISPAELQEQIKSISDDSREVLHSMDEIVWVVNPQNDTLEHATSYLA
                     QFAQDYFSMTGVECDLVMPAQMPPHPVSSQIRHHLFLAVRESLANILKHSGATSAKIS
                     MACDNGSLDICVQDNGKGFDSKTKMANGSCSMDTQDGLRNMTKRITDVGGRCVIESAV
                     GSGTTIRFILPLKTDEKEMLYDNGFHS"
     gene            2979..3614
                     /locus_tag="VG754_12350"
     CDS             2979..3614
                     /locus_tag="VG754_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF20055.1"
                     /translation="MITVSIVDDEAKLRESIATFVNGSAGFKCVSSYGSAEAALKHLP
                     ADKPDVVLMDINLGEISGIECVERLKKQFPDMQILMLTVYEDTEKIFRALAAGANGYL
                     LKRSSPTKLLAAIREVHSGGSPMTSSIARKVVASFQKRKPAADATGEVTGQLSPREQA
                     VLDGLAKGWTYKQLASELDISIDTIRTYIRRIYEKLHVQSRTEAVAKYLQS"
     gene            3860..>4907
                     /locus_tag="VG754_12355"
     CDS             3860..>4907
                     /locus_tag="VG754_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20056.1"
                     /translation="MPKTHQDYWFSRLRKRDYLDRSGQLIEIPNWQVRLKKDGREAWF
                     NLGTANQVVAAKKAKEIFTFLEANRWDATFAKFKYDSDIAPRLNLSVGDYLRAVEATG
                     HLRIRTFLNYQNCFRTILSQAFGIKSDASKYDYRSADNGNRKWAGRVDNIRLERVTPA
                     RIIQWQRQRIKEAGHSPAAIASAKRTINSYTRCARSLFSRDIIKQLKNVKLPSSLPFD
                     GVELLDSGSMKYVSKVNVRALIAAAKEELKAAEPEVYKAFLLGLFAGMRRGEIDLAQW
                     NMIDWQNKTIVLEETEWLHLKTHDSAGVITIDEEVLNELRELMPQSKTLFIVESLVTW
                     RNGKRQRSRIRPPRN"
BASE COUNT         1186 a         1322 c         1365 g         1034 t
ORIGIN      
        1 ggattcgagc agcacccaac ccggcagcgg cgaagcggac ctggcccaaa gcaaagactc
       61 cctgcaattt caatcctggc tgaagaacca aggattgccc cgcgtgacag tggacgccat
      121 ccaacaaacg agtgacgggt atctctggat tgccgctggc ggctccattg cgcgttttga
      181 tggtgtgaga ttcgttccgt taaacgcgca tgtctcggaa acgaattttt ccacacaaac
      241 tattttcggc gacagtcagg gcgcgctatg ggtggccgtt tcaaacggtt tgcaacgctg
      301 ggaaaacggg catgcaacca tgttgacttt gaatgatggc ttgcccaaca cccatatcac
      361 cgccttgaac gaggacgggc agggtaatat ttggattggc acgacgagcg ggttggcaat
      421 ttggaacaac gggcggcttg ttccgctggc ggatgcgaaa aggattcgcg gccgcgctat
      481 cactgccatt ttcagggacc gaaagaactc gatgtggctg gtggtgcaag gaacggaagt
      541 ttttcagttc gaccacgacc atttctcgcc cctcaccacc gattcagcca aagattggat
      601 gacggacatc catgcattgc tcgtggacgc catgggcaga gtctggctgg ccaccggcga
      661 ggacgctgtc ttgtgccgcg acaaggatgg ctggcgccat tatcgcattc cgaaacgaac
      721 aacgggttct cgagtgaacg caatcgcaga ggaacctgac ggcacggttt gggccggtgg
      781 cgccggcggg ttgtttttgt tttcaaacgg taaattttcc gccgtttctc ccagcagcag
      841 actcgcgggg aacacggttc gttccttgtt cgtggcccac gatggcgcgc tgtgggcagg
      901 gacagacgaa ggtttgaacc gattgcagca caaatgtctt tttgcgctgg gacagggcga
      961 tggcctgggt ttcggcccgg tgcaagggct cgcgcaggtt tcacccggtg ttgtttgggc
     1021 agcacgaacg ggcgatggca tctatcgctg ggacggacgc agcttcaaca ggttgagggc
     1081 cgccggactt tcggcgcata attcgcaggc aaacgcgttg ctcctcgcgc gtgatggcgc
     1141 gtgttgggtc gccagctacg gcggtttgtt gcgttacaag gacccggtcg ccgcggccga
     1201 tgaagttcgt tggtttgaac ttccgggcga ggacatcatc tcactcgccg aggaccagga
     1261 cggctctttg tgggccggca ctcgcgccgg aaaactttgg caattgcgcg aagggaagtg
     1321 gctggaacaa aattcgtttt ccatttccag ttcgataaac gtcattctgc cggcccccga
     1381 tggttctgtc tggattggca ccgggggagc cggcttgatt cgcatggacc acaacgtcgc
     1441 aacatttttg ggaccaaatc gaggactccc gaacaaggtc gtacgtgcgc tgtacctgga
     1501 tgcccagggc gtcctgtgga ttggaactgc agccggcttg agctgcatgc gcagcggtaa
     1561 aatttcaaat ttcaccgccg gcgacggtct gcccgaaaac ccggttttgc agatattgga
     1621 agactccacg gggagactct ggctgggcac cagccagggg atcgcttgcg tgcacaaaga
     1681 tcaattggag aattttgcgg ccggcaagat cgaggcgctc catccaaaaa tcttcaatca
     1741 cgctgacggg atgacgtccg aggaatgcac cggcggcttt tgttgcatcg ccttgagcca
     1801 gtcgggtctt ctttggtttc caacgaccaa aggcgtgact gtgatcgcac cacagagctg
     1861 gccggtggaa aagcccttgc ccggcaccgt gatcgaagaa atcttgctgg atggcgttcc
     1921 agaaagcgcg agttccgaca acgcgaagct cacaatccca gcaggcaagc accggttgga
     1981 attcgtttac accggccttc gcttcgatgc gccggggacc attcgattcc gctacaaact
     2041 cgaaggctgg gatgctgatt ggatagacgc agggaccagc cgcagcgcgg tttataattt
     2101 cgtcccgccc ggccaatatc gttttcgggt catcgccagc aacagtgatg gcatttgggc
     2161 caaccgcgga gcggagattc ggttgatgtt cgcaagatat ttctggcaat cgtggtggtt
     2221 catcggcctc gcaggcatca ccttgttgtc cgcagtcggc ggcactgtgc gcttgattga
     2281 gcgacagaaa acaaagtccc ggctgaaacg actggaacaa gagcgggctt tggaacggga
     2341 gcgcacccgc atcgcgcagg atctacatga tgaaatgggc gcgaagcttt gccggatttc
     2401 cttcttgagt gaacatgcgc gtcgggacag catcagcccg gccgaactgc aggagcaaat
     2461 caaatccatc tccgacgatt cacgtgaagt gttgcactcg atggacgaaa tcgtctgggt
     2521 cgttaatcct cagaacgaca cgctggaaca tgcgacctct tacctcgccc aattcgccca
     2581 ggattatttt tccatgaccg gcgtggagtg tgatctggtc atgcccgcgc aaatgccgcc
     2641 gcatccggtg tcctcgcaga taaggcacca tctttttctc gccgtacggg aatcgctggc
     2701 caatatcttg aaacattcag gcgccacgtc cgcgaaaatt tccatggcct gtgataacgg
     2761 cagtctcgat atttgtgtgc aagataatgg caaaggtttt gactcaaaaa caaagatggc
     2821 caacggttct tgctcaatgg atacacaaga tggtttgcgg aacatgacca aacgaatcac
     2881 cgatgttggc ggaagatgtg ttattgaatc cgccgtcggt tccggcacga ccattagatt
     2941 catcctgcca ctgaaaactg acgaaaaaga aatgctatat gataacggtt tccatagttg
     3001 acgacgaagc caagctgaga gaatccatcg ccacctttgt gaacgggtcc gcgggtttca
     3061 aatgcgtgag cagttacggc tcggccgaag ccgcgctgaa acacctgccc gccgacaaac
     3121 ctgacgtggt gctcatggac atcaaccttg gagaaataag cgggattgag tgcgtggagc
     3181 gattaaagaa gcaattcccc gacatgcaaa tcctgatgct cacggtgtat gaagacacag
     3241 aaaaaatctt tcgcgcgctg gcagcaggcg ccaatggtta tcttttgaaa cgctccagtc
     3301 cgacaaaatt acttgccgcc atccgggaag ttcactccgg cggttcacca atgacaagct
     3361 ccatcgctcg caaggtcgtg gcgtcatttc aaaagcgaaa accagccgcc gacgctaccg
     3421 gagaagtaac ggggcaatta tcgccacggg aacaagctgt gctggatggc ctggcgaaag
     3481 gctggactta caaacaactt gcgagcgaac tggacatcag cattgacacc atccgcactt
     3541 acatccggcg catctacgaa aaactccatg tgcagtcgcg caccgaagcg gttgcgaaat
     3601 atttgcagtc gtaaattatc ggatgatggt gcatcgggca ggactcgaac ctgcaacctt
     3661 ctgatccgaa gttctaggat tgactattcg caactattct gacaaattca gtttcgttca
     3721 atagacacgg gcatttccca ataaaaacgg actcactatg caagccacga gaatacacaa
     3781 aagaacactt gagaacaacc aaaaggggac agaaagggga cagacacctt tgccgccctc
     3841 tggcaaaatt gttcgacgct tgccaaaaac acaccaagat tattggtttt cccgtctgcg
     3901 gaagcgcgat tatcttgacc gcagcggcca gcttattgag attccgaatt ggcaggtgcg
     3961 cctcaaaaaa gacgggcggg aggcatggtt caacctaggc acggcaaatc aggttgtggc
     4021 cgcgaagaag gcgaaggaga ttttcacttt ccttgaggcg aaccgatggg atgccacgtt
     4081 cgccaagttc aaatatgact ccgacatcgc gcctcgcctc aatctctccg ttggcgatta
     4141 tctccgcgcc gtcgaagcca ccgggcacct tcgcatccgc acattcctga attaccaaaa
     4201 ctgtttccgc acaatcctca gccaagcctt cggcatcaaa agcgatgcgt ccaaatacga
     4261 ttaccgcagt gcggacaatg ggaaccgcaa atgggccggg cgcgtggaca atatccgttt
     4321 ggagcgcgtg accccggctc gcatcatcca atggcagcga caacgcatca aggaagccgg
     4381 gcactcaccc gccgcaatag cttccgccaa gcgcaccatc aacagctaca cccgttgtgc
     4441 ccggtccctg ttcagccgcg acatcatcaa acaactcaag aacgtcaaac ttccatcttc
     4501 cctccccttt gacggcgtgg aactgctcga ttcaggcagc atgaaatacg tttcaaaagt
     4561 aaacgtgcga gcactcattg ctgccgccaa ggaggagttg aaagcagccg agccggaagt
     4621 ttacaaagcg ttcctgcttg gtctgtttgc cggtatgcgc aggggagaaa ttgatctggc
     4681 gcaatggaac atgattgatt ggcaaaataa gaccattgtg ttggaagaaa ccgaatggct
     4741 gcacctgaaa acccacgaca gcgccggtgt tatcaccatt gatgaggaag tattaaacga
     4801 attgcgcgaa ctgatgccgc agagcaaaac gcttttcatc gtcgagagtc tggtaacatg
     4861 gcgcaatggc aaaagacagc gcagccgcat ccgtccgccg cgcaata
//
LOCUS       DASZMM010000514         9262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_786465, whole genome shotgun sequence.
ACCESSION   DASZMM010000514 DASZMM010000000
VERSION     DASZMM010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786465"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..916
                     /gene="ispH"
                     /locus_tag="VG754_12360"
     CDS             53..916
                     /gene="ispH"
                     /locus_tag="VG754_12360"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWF20057.1"
                     /translation="MKIIRAEHRGMCFGVRDAIALAKNTRAPQTILGDLVHNETVLAD
                     LRARGIQIKQKPDEVATQDVMITAHGASERAVQEIKRRGLNVLEATCPLVRFAHEAIA
                     ELAAAGFHPVIIGKRDHVEVRGLTGDLDAFDVVLDEADVMKLPERARFGIAAQTTQPI
                     EKVERWVALIRARFPKSEVRFVDTVCRPTKQRQAAAVELARQSDVVVVIGGARSNNTH
                     ELAATCRRHCARVHHVQGAEDLRAEWFEGAQIVGITAGTSTPDKLIDEVELWLRHQKL
                     CRQKQPNKEES"
     gene            913..1716
                     /locus_tag="VG754_12365"
     CDS             913..1716
                     /locus_tag="VG754_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20058.1"
                     /translation="MNHAKWINYFKRNRLDRPEPDWTAPITVQPRVLAPLLKSLEQFR
                     LGDGGGPASLIAHDAEIFRGSTEEMRAIVDLWFREEAEHARLLGCAVDRFRGRRITSH
                     WSFSAFCLCRRAIGVKGELQILLLTELVSTAYYRVLCAHVPDAPVADMCRLILRDEAG
                     HVAFHRERLADAGRSSVGLGGALWQAQFWAFGHAAATMLWVNHGACLTAIGGSRSEYF
                     HEVRRQLRRFILLLDRMSESLLEQVYAAAFSSPDSGGTALATVRPRNCS"
     gene            1713..1895
                     /locus_tag="VG754_12370"
     CDS             1713..1895
                     /locus_tag="VG754_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20059.1"
                     /translation="MIEDIERESQATGKSPEPADKDVCATWRRLSSLRVAGTFQSPLQ
                     NSAKIKMRPMRNGLRN"
     gene            1933..2640
                     /locus_tag="VG754_12375"
     CDS             1933..2640
                     /locus_tag="VG754_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20060.1"
                     /translation="MKERKRWLTVVAYAIAMAWVESAVVFYLRTLSDRIDPHQTEPLP
                     PVPGITGVELVREAATLLMLCTVGMLAGKNWRSRWGYAAIAFGVWDIFYYVFLKVMTG
                     WPNSLTDWDVLFLLPLPWWGPVIAPVMIASLMIIWGTLASQWEHQRPFRSEWRAWGLN
                     FAGCALAMFVFMEDAIRIVGKGEDALRLLFPVRFDWPLFCVALMLMTAPVLGNGRHIR
                     RARVNLPEPKLNQEPNT"
     gene            2698..3408
                     /locus_tag="VG754_12380"
     CDS             2698..3408
                     /locus_tag="VG754_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20061.1"
                     /translation="METNSTSDSFRQVSVILRGEPEPIDQWIRQWDAGRMALYVLVIV
                     VGTGFYGAAMGYWRAPEQALFTAIKFPLIILLTTLGTGLLNGMLAPLLGLSINFRQSL
                     QAVLMSFMISGAILGSFSPVVFFVIWNSPSMSAYASRPYSTIMLLHVALITFAGVAAN
                     LRLGQLLQRLSGSVAISRRVLFAWLAVNFLLGSQLSWILRPFIGSPNLPVEFLRKNAF
                     DGNFYEAVFNAIKQISFP"
     gene            3440..4249
                     /locus_tag="VG754_12385"
     CDS             3440..4249
                     /locus_tag="VG754_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20062.1"
                     /translation="MNDQNPPPHPSSSPGFSNPAIPPFIPPAPSPSGAALAGDAAERV
                     PITGVISAIEAILRQPRRVMFQVRQPKPGSLIAALLIITVVCSLIYGIVVGTFSGAEQ
                     YWAAPVKIVAGLMISALICLPSLYIFSCLGGSQARLVEVFGLLAGLLALMTVLLIGFA
                     PVAWVFSQSTKSVAMMGTLHLLFWFVATVFGLRFLQAGFSHFCTRSYAGLQVWMVVFM
                     LVALQMTTALRPIVGRSKTFLPAADDKRFFISHWMDCLGGPDEVKHNEAAR"
     gene            4328..5041
                     /locus_tag="VG754_12390"
     CDS             4328..5041
                     /locus_tag="VG754_12390"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HWF20063.1"
                     /translation="MNESQKNYGWARAFPLIALLFASGIGVLLSIARIVSTHNAHYAM
                     LPGNLFLAWLPLVFAMGVYHLHRNGERRSLRLGVLAGLWLLFFPNAPYIFTDLVHMWT
                     YFRFAFWLDLSLVLLVALTGFLVGFVSLYLMQSVVVERFGRLMGWLFILVVAWLTGFG
                     VYMGRFLRLNSWDALVHPLSVTHSIGHLATHPLDHPDTVTFPLLFATFLFLGYLMLYA
                     LTHLQSATQGRVQSLKFEG"
     gene            5191..5763
                     /locus_tag="VG754_12395"
     CDS             5191..5763
                     /locus_tag="VG754_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20064.1"
                     /translation="MTFDAANSREQVLLRRIKLLTWVFILGLAIGGATAIPLKTELEL
                     SVKLLGVEGKTSTEVSSGLAHWILRVREGLQETDTKYPFMAYGTDWLAFGHFMIAVAF
                     VGALRHPVRNVWLFTFGMIACALIVPYAFIFGGVRGIPIYWRLIDCSFGVFGIIPVWY
                     CRRCVAEIEQIRRSNTTKVSGNEAQKHVPD"
     gene            5799..6701
                     /locus_tag="VG754_12400"
     CDS             5799..6701
                     /locus_tag="VG754_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF20065.1"
                     /translation="MALIQKSNQSYQSDFAKQRRRASPTICERRARASSRRLLLVTNG
                     SGSFAFTLVELLVVIAIIAILASLLLPALNKSKVQAEGIACNNNIRQLSIAWSLYADD
                     NDDLLVNNYGKPQTLLTRNTWANNVESWTNSDDNTNLTYVTDTLFSPYDNRNGKIFKC
                     PSDRAAASNGERIRSMSMNAMVGDPGDLLDNFNPLYVQFLKLADIRNPAGIFVFLDEH
                     CDTINDGFFVNRLEDYQWGNLPASYHNGAVNLAFADGHFEAHRWQIGDTVRPPVQGGV
                     GGTFAATPTTDFEWLKQRTTMKKQ"
     gene            6705..7298
                     /locus_tag="VG754_12405"
     CDS             6705..7298
                     /locus_tag="VG754_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20066.1"
                     /translation="MPDYPEKDFPWTFYFNGVAMPKWGACWPEFSAGEHRHWDWEVGP
                     RDFYIIRCKINHVGTIESANPHAFLYAVQELLCLLFTEQKTVSEYCRRLAKPTVTPEE
                     IYQGLIDAAFEMRERSRRDAVAFWTSGYDADRLRLVEAMRRATLPANDPEHQPPPHMQ
                     SRATHLKTLWQLQLKTLHQAANAKNIPAAMRKKLSDL"
     gene            complement(7304..8206)
                     /locus_tag="VG754_12410"
     CDS             complement(7304..8206)
                     /locus_tag="VG754_12410"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWF20067.1"
                     /translation="MNEPLLTLELPGTRKLKSGKVREVFDLGDRLLFVASDRISAFDC
                     IMPNGIPRKGEVLTQISYFWFAQTAAFQPNHLFTGADDVLGVPALAGLPPELSTRLKG
                     RSMIVKKAQPLAIECVVRGYLAGSGWKEYRERQTVCGIKLPAGLQESAELPEPIFTPA
                     TKAETGHDENISFHEAAKLVGGDIAEQARAASMKIYKFAREYARQRGIIIADTKFEFG
                     LFDGKLLLIDEVLTPDSSRFWPADQYRPGCGQPSFDKQFVRDYLETLDWNKTPPAPAL
                     PPEIVAKSTAKYLEAYERLTGKKL"
     gene            8316..9260
                     /locus_tag="VG754_12415"
     CDS             8316..9260
                     /locus_tag="VG754_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF20068.1"
                     /translation="MNFLVTGGAGFIGSHVCERLLQGGHAVWTLDDLNSFYNPAIKER
                     NIAEIQALGKPFTFIKADVCDAAAINKLFAGVKFDQIIHLAARAGVRPSLEDPALYQQ
                     VNVGGTVNLLEAARCQGVKKILIASSSSVYGVNEKVPFAESDPVFSAISPYAATKLAC
                     ESLGHVYHHIYGMDVAMLRFFTVYGPRQRPDLAIHKFASLINAGKPIPVFGDGSTARD
                     YTYITDIVDGVMACTERQFGFEIFNLGESQTVTLNRLIELLEQSLGRKAIIQRLPPQP
                     GDVPITFADISKSRQKLGYAPKVKIEQGIPLFIQWFKK"
BASE COUNT         1908 a         2397 c         2770 g         2187 t
ORIGIN      
        1 acaaaacgaa gccctcggag tctcacactt tgtgtcacaa agtcgtttta acatgaaaat
       61 catccgggca gagcatcggg gcatgtgttt cggcgttcgg gacgccatcg cgctcgcaaa
      121 aaacacccgc gcgccgcaga ccatccttgg cgaccttgtc cacaacgaaa ctgtccttgc
      181 cgatctgcgc gcccggggca tccagatcaa gcaaaagccg gacgaagtcg ccacgcaaga
      241 cgtgatgatc accgcgcatg gcgcttcgga acgcgccgtg caggaaatca aacggcgcgg
      301 gttgaacgtg ctcgaagcca cttgtccatt ggttcgcttc gcgcatgaag cgatcgcgga
      361 attggcggcg gcggggtttc atccggtcat cattggcaaa cgcgaccatg tggaagtgcg
      421 cggtttgacg ggcgatttgg acgcttttga cgtggtgctc gacgaagccg acgtgatgaa
      481 gctgccggaa cgcgcgcgtt ttggcatcgc ggcgcagacg acgcagccga ttgaaaaagt
      541 ggagcggtgg gtggcgttga ttcgggcaag atttccgaaa tccgaggtgc ggttcgttga
      601 caccgtttgc cgcccgacga aacagcgcca ggcggcggcg gtggaattgg cgcggcaaag
      661 cgacgtggtg gtggtcatcg gcggcgcgcg cagcaataac acgcacgaac tggcagcaac
      721 ctgtcggcgg cattgcgcgc gggtgcatca cgtgcaaggc gcggaagatt tgcgcgcgga
      781 atggtttgaa ggggcgcaaa tcgtgggcat cacggcaggg acttcgacgc cagacaagtt
      841 gattgatgag gtcgaattgt ggcttcggca ccaaaagctt tgccgtcaaa aacaaccaaa
      901 caaggaggaa tcatgaacca tgcaaagtgg atcaattatt tcaaacgcaa ccgtctcgac
      961 cggcctgagc cggattggac tgcgccgata acggtgcagc cgcgcgtcct ggcgccgttg
     1021 ctcaaatcgc tggagcaatt ccggttgggc gatggaggag ggccggcatc gctgattgcg
     1081 catgacgcgg aaatatttcg cggcagcacg gaggaaatgc gcgccatcgt tgatctctgg
     1141 tttcgcgagg aagcggaaca cgcgcggttg ctcggttgcg cggtggaccg ttttcgcgga
     1201 agacgcatca cgtcgcactg gagtttctcg gcgttttgtc tttgtcgccg agccatcggc
     1261 gtgaaaggcg aattgcaaat cctgctgctg acggaactgg tcagcacggc gtattaccgc
     1321 gtgttgtgcg cgcatgtgcc ggacgcgccg gtcgccgaca tgtgccgctt gattttgcgc
     1381 gatgaggctg ggcatgtggc atttcatcgc gagcgattgg cggatgcggg gcgttcttca
     1441 gttggcttgg gaggcgcgct ttggcaggcg caattttggg cgttcggcca tgcagcggcg
     1501 acgatgctat gggtcaatca tggtgcttgc ctcacggcca ttggcgggtc gcgtagcgaa
     1561 tattttcacg aagtgcgccg gcaattgcgg cgttttatat tgttgctcga ccgcatgagc
     1621 gagtcgcttc tggaacaggt gtatgcggcg gctttttcgt cgcctgattc gggagggact
     1681 gcgcttgcaa ccgtgcgacc tcggaactgt tcatgattga ggatattgaa cgcgaaagtc
     1741 aggcgactgg aaagtcgcct gaacccgcag acaaggatgt ctgcgctacg tggcgcaggc
     1801 tttccagcct gcgggtcgcg gggactttcc agtccccgct ccaaaacagc gccaagatca
     1861 agatgcgtcc gatgcggaat gggttgcgca actgattgga aaatccgttc cagtgggtta
     1921 aaaatagcga ttatgaaaga aagaaaacgc tggctgaccg tggtggctta cgccatcgcc
     1981 atggcgtggg tcgaatccgc cgtggtcttt tatctgcgca cattgtccga ccgcatcgac
     2041 ccgcatcaaa ccgagccgtt gccgccagtc cccggcatca ccggcgtgga actcgtgcgc
     2101 gaggcggcga cgttgctgat gctttgcacc gtcgggatgc ttgccggaaa aaattggcgc
     2161 agccgctggg gttacgccgc gatcgcattc ggcgtctggg acatctttta ttacgtcttc
     2221 ctgaaagtca tgaccggctg gccaaattcg ctgaccgatt gggacgttct ttttctgctg
     2281 ccgttgccgt ggtggggccc ggtgatcgcg ccggtgatga tcgcgtcgct gatgataatt
     2341 tggggaacac tcgcgagcca atgggaacat caacgcccgt tccgctcgga atggcgcgcg
     2401 tggggactga acttcgccgg atgcgcgctg gcaatgtttg tgtttatgga agacgccatc
     2461 cgcatcgtgg gcaagggcga ggacgcgttg cggctgctgt ttccggtgcg attcgattgg
     2521 ccgctgtttt gtgtcgcatt gatgttgatg accgcgccgg tgctgggcaa cggacggcat
     2581 atccggcgag cgcgagtgaa tctcccggaa cccaagctta accaggagcc gaacacctga
     2641 aactgaaagc catggcttca ataatcgtca aaatgatgtc ctcagctcaa taacggaatg
     2701 gagaccaatt caaccagcga cagctttcgg caagtcagcg tgattttgcg cggcgaaccg
     2761 gaacccattg atcaatggat tcggcaatgg gatgcgggcc gtatggcgct ttatgtgtta
     2821 gtgatcgttg tcggaaccgg attttacgga gcagcgatgg gatattggcg cgcgccggaa
     2881 caagcccttt tcacggcgat aaaattcccg ttgatcatcc ttctaacaac gcttggaacg
     2941 ggcctgctca acgggatgct cgcgccattg ctcggattga gcatcaattt ccgccaatcg
     3001 ttgcaggccg tcttgatgag tttcatgatt tccggcgcga ttctcggttc gtttagtccc
     3061 gtggttttct tcgtgatctg gaactcgcct tccatgtcgg cttatgccag ccgcccctat
     3121 tcgacgatca tgttgttgca tgtcgcgttg ataacattcg ctggagtggc ggcaaacctt
     3181 cgccttgggc aacttttgca acgactcagc ggcagcgtcg cgatcagccg gcgcgttttg
     3241 ttcgcgtggc ttgcggtcaa ttttctcctc ggcagccagc tctcgtggat tctccggcca
     3301 ttcatcggct cgccgaattt gccggtcgaa ttcctgcgga agaacgcctt cgacggaaat
     3361 ttttatgaag cggtttttaa tgccatcaaa caaatcagct ttccctgaag aactgaaaac
     3421 aaactgaacc tcacatgcca tgaatgatca aaatccacca cctcatccgt catcctcacc
     3481 aggattttcc aacccggcga ttccgccgtt tattccaccg gcgccttcgc catcgggcgc
     3541 ggcattggcc ggagatgccg cggagcgggt cccgatcacc ggcgtcatta gcgcgattga
     3601 agcgatttta cgccagccgc gccgcgtgat gtttcaggtc aggcagccga aaccgggcag
     3661 tttgatcgcc gcgctgctca tcatcaccgt ggtttgcagc ctgatttatg ggattgtggt
     3721 cgggactttt tccggtgcgg aacaatattg ggccgcgccc gtgaaaattg ttgccggcct
     3781 gatgatttct gcgctgatat gcctgccgag cttgtacatt ttttcgtgtc ttggcggttc
     3841 acaggcccgg ttggtcgagg tgttcggttt gctggcggga ttgctggcgt tgatgaccgt
     3901 cctgctgatt ggatttgcgc cggtcgcgtg ggtgttctcg caatccacca aatcagtggc
     3961 catgatgggc acgttgcatc tgctgttctg gtttgtggcg accgttttcg gactgaggtt
     4021 tctccaggcg ggattcagcc atttctgcac ccgctcgtat gcagggctgc aggtctggat
     4081 ggtcgttttc atgctcgtgg cgctgcaaat gaccaccgcg ttgcggccca tcgtcgggcg
     4141 ctccaaaaca ttcctgccgg cggccgatga caagagattt ttcatcagcc attggatgga
     4201 ctgcctcggc gggccggacg aggtcaaaca caacgaggct gcgcggtaat atttttttga
     4261 accaaactaa ggtgaattgc tctcatattc ggggtgcgtg atgcccgtca aaagtgtaag
     4321 gtatgccatg aatgagtcgc aaaaaaatta cggttgggcc agggcttttc cgttgatcgc
     4381 gctgctgttc gcctcgggca tcggcgtgct gttaagcatc gcaaggattg tgagcacgca
     4441 taacgcgcat tatgcgatgc tgcccggaaa cctgttcctg gcatggctgc cgttggtgtt
     4501 cgcgatgggt gtttatcacc tgcatcggaa cggcgaacgg cgcagtttgc ggctcggcgt
     4561 gctggcggga ttgtggctgc tcttttttcc gaacgcgcct tacatcttca ccgatctggt
     4621 gcacatgtgg acctactttc gtttcgcgtt ctggctcgat ttgagtttgg tgttgctcgt
     4681 ggcgttgacg ggatttttgg tcgggtttgt gtcgttgtac ctgatgcaat cggtggtggt
     4741 ggagcggttt ggcagattga tgggatggtt gttcattctg gtggtggcgt ggctgacggg
     4801 tttcggcgtt tatatgggcc gctttttgcg attgaacagt tgggacgcgc tggtgcatcc
     4861 gttgagcgtg acgcattcca tcgggcatct cgcgacgcat ccgctggacc atccggacac
     4921 ggtgactttt ccgctgttgt tcgcaacttt tttgtttctt gggtatttga tgttgtatgc
     4981 gctgacgcat ctgcaatccg caacgcaagg ccgggttcaa agtttaaagt ttgaaggttg
     5041 acattcgttt ggaagcgcgg ctcgatgagc cgcagcaacg tccaaagtaa tggcacccac
     5101 aattaataat cagacgcgag tcgcaagacg cgcattgctg ggggtcatcg acccgcgctc
     5161 cgaaccgccc accggaaaag gttgattcac atgacttttg atgccgcaaa ttctcgcgag
     5221 caagttttat tgcggcggat caagctcctg acatgggtgt tcatcctggg tctggccatc
     5281 ggcggggcaa cggcaattcc gttgaaaacg gaactggaac tgtccgtaaa attattgggc
     5341 gtggagggaa aaactagcac ggaagtttca tccgggttgg cacattggat tctgagagtg
     5401 cgcgaaggtt tgcaggaaac ggacaccaaa tatccgttca tggcgtatgg gacggattgg
     5461 ctggcgttcg gtcatttcat gattgcggtg gcgttcgtgg gcgcgttgcg ccatccggtg
     5521 cgcaacgtgt ggctgttcac gttcgggatg attgcttgcg cgttgattgt gccttacgcg
     5581 ttcatctttg gcggcgtgcg cggcatcccg atttattggc ggttgattga ttgttcgttc
     5641 ggggtctttg gaatcatccc ggtatggtat tgccggcgtt gtgtcgcgga aattgagcaa
     5701 attcgcaggt caaatacgac gaaagtatca ggaaacgagg cgcaaaagca cgttccggat
     5761 tgacgcttta cggaagctgg ttcagttttg gtcattgaat ggcgctgatt cagaagtcga
     5821 accaaagtta tcaaagcgat ttcgccaagc aacgacgtcg ggcgtctcca acgatttgtg
     5881 aaagacgagc cagagcctcc tcgcgtcggc tcctgcttgt cacgaatggg tcgggttcgt
     5941 ttgctttcac attagtcgag ttgctggtcg ttatcgcaat catcgctatc ctcgcgagcc
     6001 tgcttttgcc ggcgctgaac aaatcaaaag ttcaggccga aggcatcgct tgcaacaaca
     6061 acattcgcca gctttccatc gcctggtcgc tttacgcgga tgacaatgac gatttgctcg
     6121 tcaataatta tgggaagccg cagacacttc tcacgcgcaa cacttgggcg aacaacgtgg
     6181 aaagctggac gaacagcgat gacaacacca atctcactta cgtgaccgac acgttgtttt
     6241 cgccttacga caatcgcaac gggaaaattt tcaagtgccc atccgaccgc gcggcggcct
     6301 caaacggcga gcgcatccgc agcatgtcca tgaacgcgat ggtcggcgat ccgggagatc
     6361 tcctggataa tttcaatccg ctatatgtgc aatttctcaa gttggccgat atccgcaacc
     6421 ccgccgggat ttttgttttt ctggacgagc attgtgacac gatcaacgac ggtttttttg
     6481 tgaaccggtt ggaggattat caatggggca atttgccggc ttcgtatcac aatggcgcag
     6541 tcaacctggc tttcgccgac ggacattttg aggcgcatcg ctggcagatc ggcgacacgg
     6601 tgcgtccgcc ggtgcaaggc ggcgtgggcg gcacttttgc ggcgacgccg acaacggatt
     6661 ttgaatggct caagcaacgt acgaccatga aaaaacaatg aaccatgcct gactatccag
     6721 aaaaagattt tccgtggacg ttttatttca atggcgttgc gatgccgaaa tggggcgcgt
     6781 gctggccgga attttccgcc ggggaacatc gccattggga ttgggaagtc ggcccgcggg
     6841 atttttacat cattcgctgc aaaataaacc acgtcggcac catcgaaagc gcgaacccac
     6901 acgcgttcct gtatgcggtg caggagttgc tgtgcctctt gttcacggaa caaaaaactg
     6961 taagtgaata ttgccgtcgc cttgccaaac cgacggtgac gccggaggaa atctaccagg
     7021 ggctgatcga cgcggcgttc gagatgcggg aacgttcgcg ccgggacgcg gtggctttct
     7081 ggacttcggg atatgacgcg gaccgcttgc gtctcgtgga agcgatgcgc cgcgccacgt
     7141 tgccggcgaa tgatccggag catcaaccgc cgccgcacat gcaatcacgc gccactcatt
     7201 tgaaaacgct ttggcaactg caactcaaga cgcttcatca ggccgccaac gcaaaaaaca
     7261 ttccggcggc gatgcggaag aaattgtcgg acttgtaaaa aaattacagc ttttttcccg
     7321 tcaatcgctc gtaagcttcc agatattttg cggtgctttt ggcaacgatt tccggcggta
     7381 gcgcgggcgc gggcggggtt ttgttccaat cgagcgtttc gagataatcg cggacgaatt
     7441 gcttgtcgaa actcggctgg ccgcaaccgg gacgatattg gtccgcaggc cagaaacgcg
     7501 aggaatcagg cgtgagcact tcgtcaatca acaacaattt gccgtcgaag agtccgaatt
     7561 cgaatttggt gtccgcaatg atgatgccgc gctggcgcgc gtattcgcgg gcgaatttgt
     7621 agattttcat gcttgccgcg cgcgcctgtt ccgcgatgtc gccgccgacc aatttggcgg
     7681 cttcatggaa ggaaatattt tcgtcgtggc cagtttcggc ctttgtggcg ggagtgaaaa
     7741 tgggttcggg caattcggcg gattcctgca agcccgcggg caacttgatg ccgcagacgg
     7801 tttggcgttc gcgatattcc ttccagcccg acccggcgag atacccgcgc acaacgcact
     7861 cgatggcgag cggttgggct ttcttgacga tcatggaacg gcctttaagc cgggtggaca
     7921 attcaggggg aagaccggct aaagccggga caccaagcac gtcgtccgcg ccggtaaaaa
     7981 ggtggttcgg ctgaaatgcg gcggtttgcg cgaaccaaaa atacgagatt tgcgtgagca
     8041 cttcgccttt tcgcgggatg ccgttgggca tgatgcaatc gaatgccgag atgcgatcac
     8101 tcgcgacaaa caagagacgg tcgccgaggt caaacacttc gcggaccttg ccgcttttca
     8161 atttgcgggt gccgggcagt tcgagcgtca aaagtggttc gttcaacacg attaaaatat
     8221 ggagttcaaa ctttcaggtt taaagtttaa agttgccgcc gaagcgcaat acgcatacgc
     8281 atctgccatc aactttgaac attgaacttt aaaccatgaa ctttttagtc accggcggcg
     8341 cgggatttat cggctcgcac gtatgcgaac ggttgctgca aggcggtcat gccgtctgga
     8401 cgctggacga cttgaattca ttttacaacc cggccataaa ggagcgaaac attgcggaaa
     8461 tccaggcgct cggtaaaccg ttcaccttta tcaaagccga tgtttgcgac gcggcggcga
     8521 tcaataaact ttttgccggg gtgaaatttg atcaaatcat ccaccttgct gcgcgcgccg
     8581 gtgtgcggcc cagtttggaa gaccccgcgc tttatcagca ggtcaatgtc ggcggaacgg
     8641 tcaacttgct cgaagcagcg cggtgtcagg gcgtaaaaaa aatcctcatc gcctcttcgt
     8701 cctcggttta tggcgtgaac gaaaaagtgc cgtttgccga aagcgacccg gtgttttccg
     8761 cgatttcgcc ctacgcggcg accaagctgg cgtgcgaatc cttgggccat gtgtatcatc
     8821 atatttacgg gatggacgtc gcgatgctgc ggtttttcac ggtatatggt ccgcgtcaaa
     8881 ggccggattt ggcgatccac aaattcgcga gtttgataaa cgccggcaaa ccgattccgg
     8941 tcttcggcga tggttcgacc gcgcgcgatt acacttacat cacggacatc gtggacggcg
     9001 tgatggcctg caccgagcgg cagtttggtt ttgagatttt caacctgggc gaatcgcaga
     9061 cggtgacatt gaaccggctg attgaattgc tcgaacaatc gttgggacgc aaggcgatca
     9121 tccaacgcct tccgccacag cccggcgacg tgccgatcac tttcgcggac atcagcaaat
     9181 cgcgccagaa actcggctat gcgccaaagg tgaagattga gcagggaatt ccgctgttta
     9241 ttcaatggtt caagaaatga tt
//
LOCUS       DASZMM010000515         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1353559, whole genome shotgun sequence.
ACCESSION   DASZMM010000515 DASZMM010000000
VERSION     DASZMM010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353559"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VG754_12420"
     CDS             complement(<1..746)
                     /locus_tag="VG754_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF20069.1"
                     /translation="MKKKHATVKKRITAPRLVKRGRPRKRFDLEPAEKTKSPAVTIAE
                     TDEVEEADEETATSVPRDVDTDVVAERERNPYDGNTAFNLYLREVGQTKLLTIAEENA
                     LAARIKKGDKKAREQMIKANLRLVVKIAREYEDYGMPLLDLINEGNMGLMKAVERFDP
                     AKGAKLSTYSAWWIKQAIKRALANQSKTIRLPVHVVDKLFHMRQASVKLHEVLGREPT
                     DEELGGELGFTAQKVAQLRTAAIRPASLEAP"
     gene            complement(1071..2516)
                     /locus_tag="VG754_12425"
     CDS             complement(1071..2516)
                     /locus_tag="VG754_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 79 C-terminal
                     domain-containing protein"
                     /protein_id="HWF20070.1"
                     /translation="MKIFSLVICAAWLLTDAALAQSPVTLTIGNVPGREIPAGFCGLS
                     FGAVGELEGHGGVDGHLFSPTNTQLITLFKNSGLRHLRIGGSTVEGANAAVPDHEAID
                     NVFGFARAADVKVMYSLRLLNGDPAVAAADARYIWEHHRAMLDCFAIGNEPDIKRYHY
                     PPFGTGSDPAITNYTSYLAAWRKFAAAVTNAVPDAKFAGPDAANRSWAGRFAEDEKNF
                     GIVPLITQHLYVGGSPWLKPGGPETIPAAEAISNMLSEKWVNQKYPAFYKEAVVPIEK
                     QDIPFRMTEANDYLKGVRGASDAFASALWALDYLHWNAAHETNCVGVNFHNTEWLRTD
                     TVYLDSDGNYQIHPKAYGIRAFDLGSRGQVEKVKIQNKEKLNLTAYAVGDTTNLFVTV
                     INKEYGEDARNAAVSIKTEGFASGSVEAIFLQAKNGDVGATNGITLGGATMGNGSLWA
                     GKWSSATQFVHGECRITVPAACAVIVKVSVP"
     gene            2887..>3303
                     /locus_tag="VG754_12430"
     CDS             2887..>3303
                     /locus_tag="VG754_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20071.1"
                     /translation="MKRGVIILAFFAGGVLLIGILIALSAGGPRRDLEKTKEMLRREG
                     YKTELAEFNFVTTPEMRARAAAFTPNRTIHSGPGINRLDFMAPVGQDTALVLWQLPEL
                     PSHNAEDGWPALRESLDEDRSSLDAAMNAILSGPLKF"
BASE COUNT          697 a          968 c          849 g          789 t
ORIGIN      
        1 ggcgcttcga gcgaagcggg acgtatcgcg gcggtgcgca attgcgcgac cttttgcgcg
       61 gtaaacccaa gttcaccgcc caattcttca tcggtcggtt cgcgcccgag cacttcgtgc
      121 agcttcaccg acgcctgccg catgtggaac aacttgtcca cgacatggac cggcaaccgg
      181 atggtcttgg attgattggc caatgcgcgc ttgatggctt gtttgatcca ccaggcgctg
      241 taggtcgaga gcttggcgcc cttcgcggga tcgaaccgtt cgacggcctt catcaagccc
      301 atgttgccct cgttgatcaa atccagcagc ggcatcccgt aatcctcgta ttcgcgggca
      361 atcttgacga cgaggcgcag gttcgccttg atcatctgtt cgcgggcctt tttgtcgcct
      421 ttctttatgc gcgcggccaa agcgttttcc tccgcgatgg tcaacagctt ggtctggccg
      481 acttcgcgca agtaaagatt gaatgcggtg ttgccatcat acggattgcg ttcgcgttcg
      541 gcgacgacat cggtatcaac gtcgcgcgga acggacgtgg ccgtttcctc gtccgcttcc
      601 tcaacttcat cggtttccgc aatcgtgaca gccggggact tggttttttc cgccggttcc
      661 aaatcaaaac gcttgcgcgg acggccgcgt ttcaccaacc ggggcgccgt gattctcttt
      721 ttaactgtgg catgtttctt tttcacaaaa ttccttcaag tagtgggttg ataaaaatta
      781 aaaaaatgat tcgtgcgatt caggggacgg gccatttctg aacgaccagt caactattct
      841 atttcggaca accaaagccg gtctttgggg tgcgttctcc agctccacct tccaaaaggt
      901 tcaaaagcag gaatggcccg tatttgtaag acaaaccatt acagattatg tttgaaaaag
      961 gtttcatctt ttcacttctc gctttccacc atttcacgca tcaaaatagc atactagacc
     1021 aaccggtcaa ctactttttg cgaaaattgc ttctaaaaag ctgtaagcca ttatgggact
     1081 gataccttca cgatcaccgc gcaagccgct ggcacggtaa tccggcattc accgtggacg
     1141 aactgggttg ccgaactcca tttccccgcc caaagcgaac cattgcccat cgttgctccg
     1201 ccaagtgtga tgccgttggt cgcgccgaca tctccgttct tcgcctggag aaatatcgct
     1261 tccacgcttc ccgatgcaaa accctccgtt ttgatgctca ccgccgcgtt tcgcgcatcc
     1321 tcgccatatt ccttgttgat gacggtgaca aaaagattgg tcgtgtctcc gacggcatag
     1381 gcagtcagat tcaacttctc cttgttctgg attttgacct tctccacctg accgcgactt
     1441 cccaaatcaa atgcccggat gccatacgcc ttggggtgga tttggtagtt cccgtctgaa
     1501 tccagataca cggtgtcggt tcgcagccat tctgtgttgt ggaaattgac gccgacgcag
     1561 ttcgtctcgt gcgcggcatt ccaatgcaga taatccagcg cccataatgc cgaagcgaac
     1621 gcatcgcttg cgcctcgaac gcccttgaga taatcgttcg cctcggtcat ccggaatgga
     1681 atatcttgct tctcaatcgg cacaaccgct tccttgtaaa acgccggata tttttggttc
     1741 acccattttt ctgaaagcat gttgctgatg gcttccgcgg cgggaatggt ttcgggaccg
     1801 cccggtttga gccagggact tccgccgacg tataaatgtt gcgtgatgag cgggacgatg
     1861 ccgaaatttt tttcgtcttc cgcaaagcgc cccgcccacg agcgattggc ggcatccggt
     1921 ccggcaaact tcgcgtcagg caccgcgttg gtgacggcgg cggcaaattt gcgccatgcc
     1981 gcgaggtaac tggtgtaatt ggtgatggca gggtcggaac cggtgccgaa cggcggataa
     2041 tgatagcgtt tgatgtcagg ttcgttgccg atggcgaaac aatccagcat cgcgcgatga
     2101 tgttcccaga tgtaacgcgc atcggcggcg gcaactgcgg ggtcgccgtt gagcaggcgt
     2161 aacgaataca tcaccttcac atcggctgcc cgggcaaagc cgaacacatt atcaatcgct
     2221 tcatgatcgg gaactgcggc attggcgcct tcgaccgtgc tgccgccgat gcgcaaatgg
     2281 cgcagaccgc tgttcttgaa caacgtgatc aactgcgtgt tcgtcggcga aaaaagatgc
     2341 ccgtccacgc cgccgtgccc ttccaattcc cccaccgcgc caaaacttaa tccgcaaaaa
     2401 cccgctggaa tctcacgccc cggaacattc ccaatggtta acgtgaccgg cgattgcgcc
     2461 aacgccgcat ccgtcaaaag ccacgcggcg caaatcacga gtgaaaagat tttcattttc
     2521 tgcatcctgc ccttgctctt atggtttgaa aagaaagaag gccaatcaaa aatgctcgac
     2581 aaaccgccgc cttttctgtc atgttttcag ggtgtggaaa gaaaggagca cacaagaaca
     2641 ccgacctatg tgttccgcag tccggtcatc ttcaaccgga agggaaaact attattgccg
     2701 caatcgcaat cctgttgcgc ccgttcgaat cgaattagcc ggcgaaattc tcgcatattt
     2761 tcccgcgcgt gaatggataa tttcccggtt attaccgcgt tttcaagcct tttaccgcct
     2821 tttttgccac cccctcctcc ctccccatcc aatcggacca tcgcagtcac cgccgggcca
     2881 ggaattatga aacggggcgt gataatcctt gccttctttg cgggcggcgt gctcctgata
     2941 ggcatactca tcgcgttgag cgcaggcggc ccgcgacgcg atttggagaa gaccaaggag
     3001 atgttgcgcc gcgaaggtta caagacggaa ctggctgagt tcaattttgt caccacgccc
     3061 gaaatgcgcg cgcgcgcggc tgcgttcact ccaaaccgga cgattcactc gggacccggc
     3121 attaaccggc tcgatttcat ggccccggtg ggccaggaca ccgcgctggt gctctggcaa
     3181 ttgcccgaac tcccgtctca caacgccgaa gatggctggc ccgcgttgcg cgaatcgctg
     3241 gatgaagacc gctcctcgct ggacgcggcc atgaacgcca ttctttccgg gccgcttaaa
     3301 ttc
//
LOCUS       DASZMM010000516         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2967718, whole genome shotgun sequence.
ACCESSION   DASZMM010000516 DASZMM010000000
VERSION     DASZMM010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2967718"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VG754_12435"
     CDS             complement(<1..328)
                     /locus_tag="VG754_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HWF20072.1"
                     /translation="MNTIQCFKSTLQKLGIIVGLLALAFAPNRLLAQRPLGIDVSSYQ
                     GTPNWSSVRGSGIVFAWAKATESTTITDGDFAYNENNGKAAGVLMGAYHFAHPNLNSP
                     GAEAGYF"
     gene            complement(592..665)
                     /locus_tag="VG754_12440"
     tRNA            complement(592..665)
                     /locus_tag="VG754_12440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(631..633),aa:Gln,seq:ttg)
     gene            complement(1032..>2675)
                     /locus_tag="VG754_12445"
     CDS             complement(1032..>2675)
                     /locus_tag="VG754_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF20073.1"
                     /translation="LAQNGGSLEARLFALWTLHSSGQLRETLLTSFFQDEEPAIRAWA
                     ARLAGERRIASEKIIEGLVKLATDTNPSVRLAVATAARQFASGSLTVDTPVSVEPQPF
                     ETGRILAPLMKASADAKDPLIPFMIWEAAEPKIGEDPTHALRWLAENGPETMPLSGKI
                     ARKVMRRICDTRNAQHLAGAMDFIGSVSGKDVALAAAALDGLLEGQQGNAFKPATDAG
                     ALLAQLMSSGKSELADRARRLGTLWGDAAAVQSTIAQINDPNVPLEERIHAIQAARNV
                     KSSAARDAMLTALSGENPEPLVLESIGGIGEIGGKEVGDELIARWKSFTPAERRAVTE
                     VLASRKPWTAALLSALEKKEIFPNEIPPTVIRALNESTDPVLRERATNAIGRFRPPDA
                     DKLKIIAEKKKMILSGKADLQAGHEVAKKTCFVCHKLYREGADVGPDLTGVGRSTLDA
                     LLANVIDPNQVVGKGYENVEVETKDGRSLSGRVVENTDTRVRLLSAGPHEEIVGKSEI
                     VSMHVTPLSVMPEGLEQMPDADFRNLIMFILNPPQDTKK"
BASE COUNT          576 a          765 c          734 g          600 t
ORIGIN      
        1 agaaataacc ggcttcagcg ccgggcgagt tcaaattggg atgagcaaaa tgataagcgc
       61 ccatcaaaac tccggcggct ttgccgttgt tttcgttgta agcgaaatca ccgtcggtga
      121 tggtcgtact ttcggtcgcc ttggcccagg cgaacacgat gcccgagcct ctgacactgc
      181 tccagtttgg cgtgccttga taggaggaga catcaatgcc gagcggacgt tgcgccagca
      241 ggcgattcgg cgcgaacgcg agcgccagca ggccgacgat gatgccgagt ttttggaggg
      301 tagatttgaa gcattggatt gtattcatga ttgcttcctt cttactgcgg gtttgttttg
      361 ggtttgttga tcttccggac acggcagcac ccggcgtttg ggcacgcttc ctttcgtgcc
      421 cagggaagtg cggcagttat ccggaattac aggcacttcc aacaacgggt gatattagcc
      481 caatgttact gcgagttact cgaatgcgcc atacaacatc cgttgtatcg ggacgagtta
      541 ttgcgggaga aattaaaaat tgatttttga ggaaaaatga actggaaaag ttggctgtcc
      601 gacatggatt cgaaccatga caaaagcctc caaagggcct tgtgctacca ttacaccatc
      661 ggacaaaccg gaacgaaaaa tagccttctc gcgctttctg cgcaagttcc gagtttgcgc
      721 atcaccacaa acgggcgcat ctggaccctg ttcttaaatc cattatgaga agcgagagaa
      781 ggctcgccct catcccggcc ttctccccct aggagaagga gacccggccc ctgcccgctt
      841 tttcattggc cgcaatggtt tgcaccatcc catcgcggga atgttccgag cagcgggaaa
      901 tggtgaaagc cctctcctgg gggagagggt ttgggtgagg gcgagcgtca tccaaaccat
      961 acctgtacat tctcaagaac agcggcaaga tgcgcccaac acaagccaat gcaccgcctc
     1021 attgcgccgg ctcacttttt tgtgtcctgc ggcgggttga ggatgaacat gatgaggttg
     1081 cggaaatcgg cgtcaggcat ttgttccaat ccttcgggca tgacggacaa cggtgtgacg
     1141 tgcatggaaa caatttccga tttgccgacg atctcctcat gcggcccggc ggaaagcagc
     1201 cgcacgcgcg tgtccgtgtt ttccaccacg cgtccgctga gcgagcggcc atccttggtt
     1261 tcgacttcca cgttctcgta gcctttccca acgacctggt tcggatcaat gacatttgcc
     1321 agcaacgcat ccagagtcga acggcccacg ccggtcaaat ccggaccaac atccgcgcct
     1381 tcgcggtata atttatggca aacaaaacag gtcttctttg ccacctcatg gccggcctgc
     1441 aaatccgcct tgccgcttaa aatcattttc tttttctccg cgataatttt caacttgtcc
     1501 gcatcgggcg ggcgaaaacg tccgatggca ttggtggcgc gttcacgcaa aaccgggtcg
     1561 gtggattcat tgagcgcgcg aatcacggtg ggtggaattt cattcggaaa aatttctttt
     1621 ttctccagcg cagataacaa ggccgccgtc caaggtttgc gcgatgccaa aacttccgtg
     1681 acagcgcgcc gttcggctgg cgtaaaggat ttccaacggg caatgagttc atcgccgact
     1741 tccttgccgc caatttcgcc gatgccgccg atgctttcca aaaccaacgg ctcgggattc
     1801 tcgccggaca acgccgtcaa catcgcgtcg cgggcagccg atgatttcac gtttcgcgcc
     1861 gcctggatcg catggatgcg ttcttcaagc ggaacattgg gatcgttgat ttgtgcaatc
     1921 gtgctttgga cggcagcggc atcgccccac aaagttccaa gccgccgggc gcggtcggcc
     1981 aattccgatt tgccgctcga catcaattgc gcgagcaaag ctccggcatc cgtggccggt
     2041 ttgaatgcgt tgccttgctg accttcgagc aaaccatcaa gcgcggcagc agcgagtgca
     2101 acgtcttttc ccgaaacgct gccgatgaaa tccatcgctc cggcaaggtg ctgcgcgttg
     2161 cgcgtgtcgc agattcgtcg catcactttg cgcgcaatct tgccgctcaa gggcatcgtc
     2221 tccgggccat tttcggcgag ccaacgcaac gcgtgcgttg ggtcttctcc aattttcggt
     2281 tcagccgcct cccaaatcat gaacgggatg agcgggtcct ttgcatcggc ggaagctttc
     2341 atcaacggcg caaggatgcg cccggtttca aacggttgcg gctcgacgga aaccggcgta
     2401 tcaaccgtca gcgaacccga cgcgaattgc cgcgccgcgg tcgcaacggc aaggcgaacg
     2461 gacgggttgg tatccgtcgc aagtttgacc agaccttcga tgattttctc ggaggcaatc
     2521 cggcgctcac ccgcaaggcg cgcggcccaa gcgcgaatgg ccggttcttc gtcttgaaaa
     2581 aatgatgtca acaaagtttc gcgcaattga cccgaactgt gcagcgtcca taacgcgaac
     2641 aaacgcgcct ccaaactgcc gccgttttgc gcaag
//
LOCUS       DASZMM010000517         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_522309, whole genome shotgun sequence.
ACCESSION   DASZMM010000517 DASZMM010000000
VERSION     DASZMM010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522309"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1977)
                     /locus_tag="VG754_12450"
     CDS             complement(265..1977)
                     /locus_tag="VG754_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20074.1"
                     /translation="MNWTQLKMVLWLRWRLTYNQFSRKGVLNTVIAIIGIVGGLSVAA
                     AAGIGSIFGGALGLSKASPQLTMFIWDGIVAAFLFIWAIGVLAELQRSETIDLARLLH
                     LPVTLEGIFFINYIVSHLTISIIIFVPAMLGLSIGLLLVNGWPMLFMFPLVLSFIFMV
                     TAWTYCLRGWLIAMMVNPRRRRNVLVAITLVIVLLGQGPNLYFNVYLRHHPHKSNASQ
                     SGHKEVPQAFITAHSYAPPLWLPNGAMQLQDGNVLPAVFGSFGAFLLGALGLARAYSS
                     TMRFYTGKEKARAPVARKIAVAGQVATIPKNFLEKRVPYVSEDVAALSLAFFRSMMRA
                     PEIRIALFTNFLVIIILVGIVFSNSLKNVQGKFVLFSSTGAVAFTFFGLIQMMFNQFG
                     FDRDGFRSLVLSPARRRDILFAKNLSFAPFAFGLGATLLIVLLFLLNLQVLGAIAALF
                     QLGAMFLFLSMAGNFVSTLVPYRISAGSLKPTKPPAKIVFLILLTQMLFPVTMLPVAL
                     PPMLGFVCENYGLLPASLVDAVFSLLLLGLAIFLYKKALDGLGDFLEKREQQILLVVS
                     QETE"
     gene            complement(1982..2209)
                     /locus_tag="VG754_12455"
     CDS             complement(1982..2209)
                     /locus_tag="VG754_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002756728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWF20075.1"
                     /translation="MAKLPIVSGTETVKALQRLGFFIDRQRGSHVVMKKVTPDGERGC
                     VIPMHREVALGTLRSALKMAGVSAEEFKDAL"
     gene            complement(2202..2414)
                     /locus_tag="VG754_12460"
     CDS             complement(2202..2414)
                     /locus_tag="VG754_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF20076.1"
                     /translation="MNMKLTAIIYPDTDTNLLVAECPEVGTASQGTTEEEALANLREA
                     TELYLESFPHALKKPAAIRTFELANG"
     gene            complement(2452..3213)
                     /locus_tag="VG754_12465"
     CDS             complement(2452..3213)
                     /locus_tag="VG754_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF20077.1"
                     /translation="MNFAIETKGLTRQFGAVRAVDGLDLQVERGRFYGFLGPNGAGKS
                     TTIKMLTGLLAPTSGLISILGEDMQDKKRAREIKRRIGVVPENLALFDNLTAREYLTF
                     VGRMYLLPLPTVRERCQELLVMMGLEQEEKKLTLEYSHGMKKKLALAAALIPGPDLLF
                     LDEPFEGVDAVASRVLRDILKRCVERGATVFLTSHVLEIVERLCTDVGIIAHGKLVYQ
                     GSMEKAREGGSLEETFLKTVGGDQHENQKLSWLEG"
     gene            3328..3516
                     /locus_tag="VG754_12470"
     CDS             3328..3516
                     /locus_tag="VG754_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20078.1"
                     /translation="MSTVKEIESAIQRLSKTEMQEIHDWLENVLEDQLEMREEFKAQI
                     EASKREMKEGKRPRVRQP"
     gene            3521..3778
                     /locus_tag="VG754_12475"
     CDS             3521..3778
                     /locus_tag="VG754_12475"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF20079.1"
                     /translation="MSRALQVYYTVFDHAFWRLPPDVRRRIEAKIDEMGTRLGTYPHY
                     RMTGTDTFRLRIGDYRVIYEFDAEKQEIYLLSVGHRREVYR"
     gene            3852..4193
                     /locus_tag="VG754_12480"
     CDS             3852..4193
                     /locus_tag="VG754_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20080.1"
                     /translation="MHPDSFGPEDFRQAFQRIRHQLSERQCDMLRAHYNAPDRKITAK
                     QLAGAVGSPNYKTANSTYGKIGSLLCEILGQEPGHIKLFVLVNFFRPENEYIWQMREP
                     AAQALKELGMV"
BASE COUNT         1141 a         1153 c         1195 g          975 t
ORIGIN      
        1 gcgttctcac gtcggctcct gccaaaggag attggaaacc aaaattcccg ggatttggca
       61 aattgccaat tatcgcggac accggctggc atcctatgtt gcgctccttg attcacgtaa
      121 ggcgagcatg tcacgggcct gaagagatgt cgaggaattt atcaggacgg gcattttgga
      181 gcggcgtgta tggatgttac aaaattacag aagcgtgtta tcccatggta aagattcaac
      241 cattaacgat gtaacctcgc gtcctcactc cgtttcctgc gacacaacca gcaaaatctg
      301 ctgctcccgt ttttccaaga aatcgccaag gccatccagg gcctttttgt ataagaaaat
      361 tgccaggccg agcagcaaca atgaaaagac tgcatccacg agcgatgcgg gcagcaagcc
      421 gtagttttcg cagacaaagc ccaacatcgg cggaagggcg actggcagca ttgtcacggg
      481 aaagagcatc tgggtgagca ggatgagaaa gacgattttt gcgggcggct tggtcggctt
      541 gagcgagccg gcgctgatcc gatacggcac aagtgtggaa acaaaattac ccgccatgct
      601 caagaaaagg aacatcgcgc cgagttgaaa taacgccgca atcgcgccca gcacctgcaa
      661 attcaaaagg aacagcaaca cgatgagcag ggtcgcgccc aacccaaaag caaatggggc
      721 gaatgaaaga tttttcgcga acaggatgtc ccgccgccgc gccggtgaca gaacgagcga
      781 ccgaaagccg tcgcggtcaa agccgaattg gttgaacatc atctgaatca atccaaaaaa
      841 cgtgaatgcc accgcgccgg tgctgctgaa aagcacaaac tttccctgca cattcttcag
      901 cgaattggaa aacacaatcc cgacaaggat gatgatgacc aaaaagttcg tgaacagcgc
      961 gatcctgatt tccggcgcgc gcatcatcga gcggaaaaat gcaagcgaca acgcggcgac
     1021 atcctcggaa acataaggca cacgcttttc gaggaaattt tttggaatgg ttgccacctg
     1081 accggcaaca gcaatcttcc gcgcaactgg cgccctcgct ttttcctttc cggtgtagaa
     1141 gcgcatcgtg gaactgtacg cccgtgccaa accgagcgcg cccagcagaa aagccccaaa
     1201 cgaaccgaac acggcgggca aaacattccc gtcctgcaat tgcatcgcgc cgttcggcaa
     1261 ccagagaggg ggcgcgtagc tgtgggcggt gatgaaggct tgcggcactt ccttgtggcc
     1321 ggactggctc gcgttggatt tatgcggatg atggcgcaga tagacgttga aataaagatt
     1381 gggaccctgc ccaagcagca cgatgacaag ggtgatggcg acgaggacgt tgcggcggcg
     1441 gcgcgggttg accatcatgg cgatcagcca gccgcgcagg caataggtcc aggctgtgac
     1501 catgaagatg aagctcaaca ccagcggaaa catgaaaagc atcggccagc cgttgaccag
     1561 cagcagcccg atcgacaacc cgagcatcgc cgggacaaat atgatgatgc ttatcgtgag
     1621 atgcgacacg atgtagttga tgaaaaaaat tccttccagc gtgaccggca aatgcagcaa
     1681 gcgcgcgagg tcgatcgttt cggagcgttg cagttccgcc agcacgccaa tcgcccatat
     1741 aaatagaaag gcggcgacga tgccgtccca gatgaacatc gtgagttgcg gcgatgcctt
     1801 ggaaagtccc aatgcgccgc cgaaaattga gccgatgccc gccgcggcgg ccacggacag
     1861 acccccaacg attccgatga tagcaatcac cgtgttcaac acgcccttgc gggagaactg
     1921 attgtacgtg agccgccagc gcagccagag caccattttg agttgcgtcc agttcattca
     1981 atcacaaggc gtctttgaat tcttcggctg acactcctgc cattttcaac gcgctccgca
     2041 aggtgccaag cgcgacttcg cggtgcattg ggatgacaca accgcgttca ccgtcgggcg
     2101 tgaccttctt cattacaaca tggctgcccc gctggcgatc aatgaaaaac ccaagtcgtt
     2161 gcaaggcttt cacagtctcc gtgccagaca cgatgggcaa tttagccatt cgccaattcg
     2221 aacgtgcgta ttgccgccgg tttttttaac gcgtgtggaa acgattcgag gtaaagctcg
     2281 gtagcttcgc gcagattcgc cagcgcttct tcttccgtgg ttccctggct tgcggtgccg
     2341 acctccgggc attcggccac cagcaagttt gtgtcagtat cgggatatat gatggccgtt
     2401 aatttcatat ttatattctg cctatttttg tgaaaaacgc aacgcagttc atcatccctc
     2461 caaccagctc aatttctggt tttcgtgctg gtcgccgccg actgttttca agaatgtttc
     2521 ctccagcgaa ccgccttcac gcgctttttc catcgaacct tgatacacaa gtttgccatg
     2581 cgcgatgatg cccacgtcgg tgcaaagtct ctcgacaatt tccaacacgt gcgaagtgag
     2641 gaacaccgtt gcgccgcgtt cgacgcaacg cttgaggatg tcgcgcaaca cgcgggacgc
     2701 gaccgcatcc acgccttcga atggttcgtc gaggaacaac aaatcgggac cgggaatcaa
     2761 cgcggcggcg agcgcgagtt ttttcttcat gccgtgggaa tattcgagcg tgagcttttt
     2821 ttcctcctgt tccaggccca tcatgacaag caattcctgg caccgttcgc gcacggtggg
     2881 cagcggcagg agatacatgc ggccgacgaa ggtgaggtat tcgcgggcgg tgagattgtc
     2941 gaacagcgcc aaattttccg ggacaacacc gatgcggcgc ttgatttcgc gcgctcgctt
     3001 tttgtcctgc atgtcctcgc cgaggatgct tatgagtccg gaggtgggcg cgagcaaacc
     3061 ggtgagcatc ttgatggtgg tggatttgcc cgcgccgttc gggccgagga atccgtaaaa
     3121 ccttccgcgc tcgacttgca gatcaagtcc gtccaccgcg cggaccgcgc cgaattgccg
     3181 cgtgagtccc ttggtttcaa ttgcgaagtt catctttggg caacgctagc cggttttttc
     3241 gatggatgcg agaggataat gaagcacgcg ttgacagcga aacgcttggt ggtattctca
     3301 agttgaacgc tattcagtaa aaagctcatg agcacagtga aggaaatcga atctgcgatc
     3361 caacgtctca gcaaaacgga gatgcaggaa atccatgatt ggctggagaa tgttctggag
     3421 gaccagttgg aaatgaggga agaatttaag gcgcagattg aagcatccaa acgcgaaatg
     3481 aaagaaggga agcggccgcg cgtgcgtcaa ccataaacgt gtgagcaggg cgcttcaggt
     3541 ttattataca gtatttgacc atgcattttg gagacttcct cctgacgttc ggcgtcgaat
     3601 tgaagccaaa attgatgaaa tgggaacacg gctcggaact tatccgcatt atcgcatgac
     3661 agggactgac actttcaggc tgcgtatcgg ggactatcgc gtgatttacg agttcgatgc
     3721 tgagaagcag gagatttatt tgttgtcagt gggacaccgg cgcgaagttt atcgctgaat
     3781 tcaataaact ctgatcaagc acgttaccca atttgctaga caccctttta ggttaagtgc
     3841 aacgtcagag tatgcaccct gattcttttg gaccagaaga ttttcgccaa gcctttcaga
     3901 gaattcgtca tcaactatcg gaaaggcaat gtgatatgtt gcgagcccat tataatgcgc
     3961 ctgatcgtaa aatcactgcg aagcaactgg cgggtgcggt aggctctccg aactacaaaa
     4021 cagctaattc gacctacggc aaaattggaa gtttgctttg cgaaatattg ggccaagaac
     4081 ccgggcacat taaacttttt gtgttggtaa acttctttcg cccagaaaac gagtacattt
     4141 ggcagatgag agaaccggcg gcgcaagctc tcaaagagtt gggaatggtt tagtgaggaa
     4201 gtttccacct gtagttttca cagtgaagta gcattcctcc tttccaacct tcgttttctg
     4261 ccttaatttc gttcgattct cagcatcatg ccaggtgctt ttatcaaaac gcacggatta
     4321 tgaattgaca ataggtggga aatcccacat atttaaatca tgccgacaat gacacaaaag
     4381 caggccctca aggtgattga aaattacgac gtcgcggctg atgcaaaaaa acgaatcagc
     4441 ctgcgcaatg ccaagacgaa atat
//
LOCUS       DASZMM010000518         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4077933, whole genome shotgun sequence.
ACCESSION   DASZMM010000518 DASZMM010000000
VERSION     DASZMM010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4077933"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VG754_12485"
     CDS             <1..903
                     /locus_tag="VG754_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20081.1"
                     /translation="RHGRITLDVPLSGRIDDPKFRAGPIIWQVVMNLIEKAATSPFSL
                     LGAMFGGGDELSFVAFQPGLATIPDTETNKLNTLTKALYERPELTLEINGSVDPELDR
                     APLAKMKFDQQIKSLYVKELTDSGKPAVAIDQVQLEPKERDRLVKRDYKLVLGKYQPS
                     PPPTNNLLTSADMAATSQGNKGLVQRLATELPEHGASLLMEQAKPIKIVSTSKIAAGT
                     NGKPAVPLTAAEILIADMETQLVQKIEITDDDLRELMKQRAAKVQAYLLQDGKITAER
                     LFITAPKPIGGSEKGTDRVNMTLD"
     gene            910..1677
                     /locus_tag="VG754_12490"
     CDS             910..1677
                     /locus_tag="VG754_12490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF20082.1"
                     /translation="MFNLTNKIALVTGAGSGIGASIAESFAQAGAFAIVADINEEAGK
                     SVAQKIQKAGGKAEFIALDVSKESACENVAKKVFQSHGHLDVLVNNAGIGCVGTLLQS
                     SVADLDRLYAVNVRGVFNVSKVFLPGMIERKNGAIINMASIGGVVGIRDRVAYCTTKF
                     AVVGLTKSMALDHSHLGVRVNCICPGRVETPFVKARLAEYADPEKAYREMSGTQLTGR
                     MLRPDEIAGAAVYLASDESASVTGACFMIDGGWSAGK"
     gene            complement(1836..>2038)
                     /locus_tag="VG754_12495"
     CDS             complement(1836..>2038)
                     /locus_tag="VG754_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWF20083.1"
                     /translation="PFSTMPRTAREDIIRTTHGALRPEGSFLVYQYSHRIFPSLKKIF
                     GQVRRDLLQAKLIPMWLFQCSR"
BASE COUNT          503 a          538 c          569 g          428 t
ORIGIN      
        1 cgtcacggcc gcatcacgct ggacgtgccg ttgagcgggc gcattgatga tccgaaattc
       61 agggccggcc cgattatctg gcaggtggtg atgaacctca tcgagaaggc ggcgacttcg
      121 ccgttctcat tgctcggagc aatgttcggc ggcggtgatg aattgagttt cgttgcattt
      181 caacccggcc tggcgacgat accggacact gaaacgaaca agctgaacac gctcacaaaa
      241 gcgctttacg aacgcccgga attgacgttg gaaatcaacg gctcggttga tcctgaactt
      301 gaccgcgcgc cgctggcgaa aatgaaattc gaccagcaaa tcaaatcgct ttatgtgaag
      361 gaactgactg attccggcaa gcctgcggtc gcaattgacc aggtgcagct cgaacccaag
      421 gagcgcgacc ggctcgtcaa aagggactat aaattggttt tgggcaaata tcagccgagc
      481 ccgccgccca cgaataattt gctcaccagc gcggacatgg ccgcaacttc gcagggcaac
      541 aagggattgg tccagagatt ggcgaccgaa ctgcccgagc atggcgcgag tttgttgatg
      601 gaacaggcca agccgatcaa gattgtttcg acttcaaaaa ttgccgcagg aacgaacggc
      661 aaacccgccg ttccattgac tgctgcggaa atcctgatcg ccgacatgga aacgcaactc
      721 gttcagaaaa tcgaaatcac ggatgacgat ttgcgcgaat tgatgaagca gcgcgccgcc
      781 aaagtgcagg cttatctgct gcaggacggc aagatcaccg ccgagcgcct gttcatcacc
      841 gcgcccaaac ccatcggcgg ttctgagaag ggcactgacc gcgtcaacat gactttggat
      901 tgattcaata tgttcaatct cacaaacaaa attgcattgg tcaccggcgc tggttcaggc
      961 atcggagcgt ccatcgctga atccttcgcg caagccggcg ctttcgccat cgttgcagac
     1021 atcaacgaag aagccggaaa atctgtcgct caaaaaattc agaaggccgg cggtaaggcg
     1081 gaatttattg ctctcgatgt gagcaaagaa tcagcgtgcg aaaacgtcgc aaaaaaagtt
     1141 ttccagtcgc atggccatct cgacgtgctg gtgaacaacg cgggcatcgg ttgtgttggc
     1201 acgctgttgc aaagttccgt cgcggatttg gatcggcttt atgcggtgaa cgtgcgcggc
     1261 gttttcaacg tcagcaaagt ttttcttccg ggaatgattg agcgcaaaaa cggggcgatc
     1321 atcaatatgg catccatcgg cggcgtggtg ggcatccgcg accgcgttgc gtattgcacg
     1381 acgaaatttg cggtcgtcgg attgaccaag agcatggcgc tggaccattc gcacctggga
     1441 gttcgcgtca attgcatttg tccaggccgc gtggaaactc cattcgtcaa ggcgcgcctc
     1501 gcggaatatg ccgaccccga aaaagcttac cgcgagatgt cggggacaca attgaccggc
     1561 cgaatgttgc gcccggacga aatcgccggc gcggcggttt acctcgccag cgacgaatcc
     1621 gcgagcgtca cgggcgcatg cttcatgatc gacggcggct ggagcgcggg aaagtgaatt
     1681 tgttttaacg cagatgaacg caaataaccg tagatgaaaa aagtgaaagt caaactttgg
     1741 aaaaattctg catttcgccc atgaaattcc actcaacaaa agtatttttc gtcgtttccc
     1801 atctgcgtgt atctgagtcc atctctggtt gcctatcacc gcgagcattg gaagagccac
     1861 atcgggatca gcttcgcctg caacaaatcg cgccgcacct ggccgaagat ttttttcagg
     1921 ctgggaaaaa tccggtgcga atattgatac acaagaaaac ttccctccgg ccgcaatgcg
     1981 ccgtgagtgg ttcggatgat gtcttcgcgc gctgttcgcg gcatcgtgct gaaaggaa
//
LOCUS       DASZMM010000519         8728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1034966, whole genome shotgun sequence.
ACCESSION   DASZMM010000519 DASZMM010000000
VERSION     DASZMM010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034966"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1628
                     /locus_tag="VG754_12500"
     CDS             129..1628
                     /locus_tag="VG754_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWF20084.1"
                     /translation="MNTLDFNKKLHEARYLMQVKRHPDALKVYAELAREFPKLGNLIE
                     EYGRAAGTAGDHVLAAQLWEKALVIGPKNADLLVRLAGEFGKIWLFDKARALSREAAE
                     LEPNNLTAQIGLASLLSRTSTVAEAREAVERCLKLDSLSEPARYFSAHLDRLENKLVE
                     AETEFRDLLASNLRDPHAIFFCHLELARVLDRMERFDEAMAQLKIAKQLTARSLNMSE
                     AQKSFDGWRTKALEKAKSLPKDILKRWAEAFPVESRCAVPPLAFLGGHARSGTTLLER
                     ILDAHPLVVAGDESLAFTTIGPMIDVTAPEIPADGLNFVRQRFLRNFRMTLGLTAEGK
                     VLVDKNPAITSYLPPFLRAFPELRVLIALRDPRDVLVSCYFETMMNYSHLSFENLAQH
                     YISVMGVWLAVREWEGLTWMETRYEDIVANLEKEGSRVTKFLGLEWHENQAIFHEKNR
                     EKPIMSTTNYTNVTSPVYARSKGRWRAYEKYLAPALPVLEPFCKEFGYS"
     gene            1885..1960
                     /locus_tag="VG754_12505"
     tRNA            1885..1960
                     /locus_tag="VG754_12505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1918..1920,aa:Thr,seq:ggt)
     gene            2042..3232
                     /gene="tuf"
                     /locus_tag="VG754_12510"
     CDS             2042..3232
                     /gene="tuf"
                     /locus_tag="VG754_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWF20085.1"
                     /translation="MAKENFQRTKPHVNVGTIGHVDHGKSTLTAAIVEVQHRKGLAPA
                     ISYADITKGGTVRDETKTVTIAVSHVEYESPTRHYAHVDCPGHADYVKNMITGAAQMD
                     GAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDLVDDPELLDLVEMEIRDLL
                     TKYQFPGDKTPIIRGSATAALAAKPEGEKAIADLLGAIDTAIPLPTREVDKPFLMCIE
                     DVFNIEGRGTVVTGRVERGELARMTEVEIVGLGETRKTTATDIEAFRKLLDKATAGDN
                     VGVLLRGTKKEEVERGMVLAKPGSITPHMKFKAEVYVLSKEEGGRHTPFFNKYRPQFY
                     FRTSDVTGNVTLPTGVEMVMPGDNVSVEVELISPVAMEKGQRFAIREGGRTIGAGRVS
                     EVVA"
     gene            3328..3403
                     /locus_tag="VG754_12515"
     tRNA            3328..3403
                     /locus_tag="VG754_12515"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3361..3363,aa:Trp,seq:cca)
     gene            3413..3502
                     /locus_tag="VG754_12520"
     tRNA            3413..3502
                     /locus_tag="VG754_12520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3447..3449,aa:Ser,seq:tga)
     gene            3517..3768
                     /gene="secE"
                     /locus_tag="VG754_12525"
     CDS             3517..3768
                     /gene="secE"
                     /locus_tag="VG754_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWF20086.1"
                     /translation="MNTGLWIIGGAVVVVFGFLWKTGQLARLKNYWDETMAEMHKCAW
                     PTWNELKGSTIVVMISIAILGGFTMLVDAILVWCARHIT"
     gene            3786..4331
                     /gene="nusG"
                     /locus_tag="VG754_12530"
     CDS             3786..4331
                     /gene="nusG"
                     /locus_tag="VG754_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWF20087.1"
                     /translation="MQSQWFVIHTLSGQEQKVKESIEKRIKAEEMEEYIHEVLVPMEK
                     VVEVRNQKKTVSTRKLWPGYVFVDMALLDDNKRIIEKPWYFIKDTQGVIGFVGDPPMP
                     TPSEEVESIKAQISDSEEREKPKVNFEVGETIKINNGPFLNFSGVIEEIEPERGKLKV
                     TVNIFGRNTPVELEYWQVEKA"
     gene            4426..4851
                     /gene="rplK"
                     /locus_tag="VG754_12535"
     CDS             4426..4851
                     /gene="rplK"
                     /locus_tag="VG754_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWF20088.1"
                     /translation="MAKKVTGQIKLQIPAGQANPAPPVGPSLGQHGVNIMAFCKEFNA
                     VTKADTGLVIPVVITVYQDKSFTFITKSPPASVLLKKAAGITSGSKTPNKEKVGKVTR
                     KQVLDIVKTKMKDLNSTSEEAGFRVVAGTARSMGIDVVG"
     gene            4909..5616
                     /gene="rplA"
                     /locus_tag="VG754_12540"
     CDS             4909..5616
                     /gene="rplA"
                     /locus_tag="VG754_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWF20089.1"
                     /translation="MPNKPSKRYKKALEQVDGKQSYPLKSAVEVLAKFPKAKFNETVE
                     VAFKLGVDPKQSDQMVRGTVPLPHGSGKTVKVLVFARNGAAADAARAAGAEFVGYEDM
                     IKKCVEGWTDFDVAIATPEAMVEVRKLGKVLGPRGLMPNPKTGTVTEDTAKAVKEVKA
                     GRVEFKLDKAGNVHVPIGKASFTPAQIAENARVVIEAVAKARPASVKGSYLQSCTLSA
                     TMSPPVRIDVREFLAAA"
     gene            5636..6148
                     /gene="rplJ"
                     /locus_tag="VG754_12545"
     CDS             5636..6148
                     /gene="rplJ"
                     /locus_tag="VG754_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWF20090.1"
                     /translation="MRAEKQNLTKEYLTRLNASPFFIVVNYQGLKVGPITELRKRLNR
                     AGSEIHVVKNSIFRLAVKEAGVGELNGALAGQIAVITGQKEVSAAAKVVKNFNAEFDK
                     LKIRFGYLNNNRLEASDLLALADLPSIEVLRSKLLGVLKAPPTKLVALLNTPASQLAR
                     VLQARVDKGA"
     gene            6303..6692
                     /gene="rplL"
                     /locus_tag="VG754_12550"
     CDS             6303..6692
                     /gene="rplL"
                     /locus_tag="VG754_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWF20091.1"
                     /translation="MAELASLVEQLGKLTVIETAELVKQLETAWGVTAAAPVAVAAGG
                     GGAAGAAAAPAEAKTAFDVILASVPADKKIAVIKAVREVKTGLGLAEAKALVEGAPKP
                     VLEGAPKAEADAAKKKLEEAGAKVEVK"
     gene            6932..>8728
                     /gene="rpoB"
                     /locus_tag="VG754_12555"
     CDS             6932..>8728
                     /gene="rpoB"
                     /locus_tag="VG754_12555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148081.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWF20092.1"
                     /translation="MPSRATERINFGKIKEIIAPPNLIELQTNSYKEFLQADIAPSRR
                     KNLGLQAVFTEVFPIESYDGKCVLDFHSYEIGEPKLDWLECLREGLTFGAPLYVTFLL
                     KEEKASKEEKVFMGELPLMTPQGTFVINGAERVIVSQLHRSPGLAFEETQHPNGKKLQ
                     SFRIIPDRGSWYEAQFDTSDLLYVYLDRKKRRRKFLTTTFFRALGYGTDEDILKLFYE
                     IEELSVKDAEKLEDLQNKVLIQDATDNDKGVVVARAFEPLSKAVIKQIAELGINKIKV
                     VDTTADEGIIIKCMKKDPAKNEDEALKDIYRRLRPGDPPTAANARALIKRLFFDPKRY
                     DLGRVGRYKINQKLGIKGGDSRILTKEDLVAATKYLLRLKKGEGSLDDIDHLGSRRVR
                     TVGELLANQCRVGLARTERLVKERMTLFDQGMESMAPQKLINPKALSAVIRDFFGRSQ
                     LSQFMDQINPLAELTHKRRLSALGPGGLSRDRAGFEVRDVHPSHYGRICPVETPEGPN
                     IGLIASLATFARINDFGFLETPYRKVEHGRVTNHVDYLTADREETFVVAQANATIDDK
                     GHFLTDKVMCRGKGDFIDVEPERVDYMDVSPKQ"
BASE COUNT         2072 a         2268 c         2538 g         1850 t
ORIGIN      
        1 atttctttac caaaagggcc gccgttattg gcggcctttt ttgctttgcg tgcaaacccg
       61 atttgcgaaa cagccgaagc tggcttttat tcttgccgtc ccagacctga aacaattttt
      121 tcttttttgt gaacacgctt gatttcaata aaaaattgca cgaggcgcgt tacctgatgc
      181 aggtcaagcg ccatccggat gcgttgaaag tttatgcgga gcttgcgcgt gaatttccga
      241 aattgggaaa tctcattgaa gaatatggac gcgcggcggg gacggcgggc gaccacgtcc
      301 ttgccgcgca actttgggaa aaggcccttg tgatcgggcc gaaaaacgcc gatttgctgg
      361 taaggcttgc cggcgaattt ggaaaaatct ggctgtttga caaggcccgc gcgctgtccc
      421 gggaggctgc ggaacttgaa ccgaataatc tgaccgcgca aatcggcctg gcgtcgcttt
      481 tgtcgcggac cagcactgtt gccgaggcgc gcgaagccgt ggagagatgc ttgaagctcg
      541 attcgcttag cgaaccggcg cgttattttt cggcgcactt ggatcgcctt gagaacaagc
      601 tggtggaagc tgaaacggag ttccgggacc tgctggcatc caatttgcgt gatccccatg
      661 cgattttttt ctgccatctc gaacttgccc gtgtgcttga ccgcatggag cggtttgatg
      721 aagcgatggc tcaactgaaa atcgccaagc agctcaccgc gcgatcgctc aatatgagcg
      781 aagcgcagaa gtcgtttgat ggttggcgca caaaagcgct tgagaaggcg aagtcgctgc
      841 cgaaggacat tttgaaaagg tgggctgaag cgtttccggt tgaatctcgt tgtgctgttc
      901 caccgctggc gtttttgggc ggtcatgcgc gcagcggcac aacgctgctg gaaagaatcc
      961 ttgatgcgca tccattggtg gtggcgggtg atgagtccct cgcgttcacg accatcgggc
     1021 cgatgattga tgtgaccgcg ccggagattc ccgcggacgg cctcaatttt gtgcgccaac
     1081 gatttctaag gaatttcagg atgacattgg gcttgaccgc cgaagggaaa gtgctggtgg
     1141 acaaaaatcc cgcaatcacc tcgtatttgc cgcctttttt gcgggcgttt ccagagctgc
     1201 gtgtgctcat cgccttgcgc gatccccgtg atgtgttggt aagttgttat ttcgaaacca
     1261 tgatgaatta cagtcatctt tcgtttgaaa acctggcgca acattacatc tcggtgatgg
     1321 gcgtctggct ggcggtccgc gaatgggagg ggttgacgtg gatggaaacc cgttacgagg
     1381 acattgtggc caatttggaa aaggaaggca gccgcgtcac gaagtttctt ggcttggaat
     1441 ggcatgaaaa ccaggcgatt ttccacgaga aaaaccgcga aaaaccgatc atgtccacca
     1501 ccaactatac caatgtgacc agcccggttt acgcacggtc aaaaggtcgt tggcgcgcct
     1561 acgagaaata tcttgcgccc gctttgccgg tgctcgaacc tttttgcaaa gaatttggct
     1621 atagttgagc gtggccattg acctaaattg ctcgccatct ttttgttatg aattggaatc
     1681 ggctggacat gacgactttt gcagaatgtc aaaataagtg aaaaaagtgg cgaaaagcca
     1741 ttgacagcta ttttaccgct gctatttttg tatccagtca ttgggccgta acagataaaa
     1801 atcatagccc ctaaactttt ttgacaaaag gtcaggggaa tggttttttg tcgcttttgg
     1861 gcgtcaggat taagttttgg ccatgcccat gtagctcagt tggcagagca cgtccttggt
     1921 aaggacgagg tcatcagttc aatcctgatc atgggctcca aattttaaga tgaagcaatt
     1981 tagatgaagt aaaaatgttg cagaaaagaa aagataacaa ccaataccag gagatcgagt
     2041 aatggcaaaa gaaaattttc aaagaacaaa gccgcacgtc aacgtcggaa ccatcgggca
     2101 cgttgaccat ggcaaatcca ccctcacggc ggccatcgtc gaagtccagc accgcaaagg
     2161 gctcgcgccg gcgatctcgt acgctgatat caccaagggc ggcacggtcc gcgacgaaac
     2221 caagacggtg accatcgcgg tttcccacgt tgaatatgaa agcccaacgc gccactacgc
     2281 gcacgttgat tgccccggcc atgctgatta tgtgaagaac atgatcaccg gcgcggcgca
     2341 gatggacgga gcgattttgg tggtcagcgc ggcggacggt cccatgcccc agacccgcga
     2401 acacattctt ttggcccgcc aggtcggcgt gccagcaatc gtggttttcc tgaacaaggt
     2461 tgacctcgtt gacgatccgg aattgctgga cctcgtcgag atggaaatcc gcgatttgct
     2521 gacgaaatat caatttccgg gcgacaagac gccgattatc cgtggcagcg ccaccgccgc
     2581 tttggctgca aagcccgaag gcgaaaaggc catcgcggac ctgttgggcg cgattgacac
     2641 ggcgattccg ttaccgacgc gcgaagtcga taaaccgttc ctgatgtgca tcgaagacgt
     2701 gttcaacatt gaaggccgtg gcacggttgt gaccggtcgc gttgaacgcg gtgaactcgc
     2761 gcgcatgacg gaagttgaaa tcgtcggctt gggcgagacc cgcaagacga ccgcgacgga
     2821 catcgaggcg ttccgcaagt tgctggacaa ggcgaccgcc ggagacaacg tcggcgtgct
     2881 gttgcgcggc accaagaagg aagaagtgga acgcggcatg gttttggcca agcccggttc
     2941 catcactccg cacatgaagt tcaaggcgga agtgtacgtg ctgtcgaagg aagaaggcgg
     3001 acgtcacacc ccgttcttca acaagtatcg tcctcagttc tatttccgca cgagcgatgt
     3061 cacgggcaac gtgacgctgc cgacgggcgt tgaaatggtg atgcccggtg acaatgtctc
     3121 cgtggaagtt gaattgattt cgccggtggc catggaaaaa ggccagcggt tcgcgatccg
     3181 cgaaggtggc cgcacgatcg gcgcaggccg cgtgagcgaa gtggttgcgt aagcagggct
     3241 tgacggtttt gtggagcgcg ggggtgaagt cctccgcctc tttttggttt gggtaaaagt
     3301 gatttgccag ccactcgacg gacgagtagg gcggtagctc aattggtaga gtagcggtct
     3361 ccaaaaccgt cggttggggg ttcgagtccc tcccgccctg ccaaccgacg ccggaggggt
     3421 ggcagagtgg tctattgcgg cggtcttgaa aaccgttaag gcctaacagc cttcgggggt
     3481 tcgaatccct ccccctccgc caacattttt atatttgtga atacaggttt atggataata
     3541 ggcggcgcgg ttgtggttgt cttcggcttc ctttggaaga cggggcagct cgcgcgtttg
     3601 aagaattact gggatgagac gatggccgag atgcacaaat gcgcctggcc gacgtggaac
     3661 gaacttaaag gttccacgat tgtcgtcatg atctccatcg cgatcctcgg cggtttcacc
     3721 atgttggtgg atgccatcct ggtctggtgc gctcgccaca tcacttaact tttttagatt
     3781 acaccatgca aagccagtgg tttgtcattc atactctgtc cggacaggaa cagaaggtca
     3841 aagaaagcat cgaaaagcgg atcaaagccg aggagatgga ggagtatatc catgaggtgc
     3901 tggtacccat ggagaaagtc gttgaagtcc gcaaccaaaa aaagacggtt tcgacccgca
     3961 agctttggcc gggttatgtg ttcgtcgata tggcgttgct ggatgacaac aagcgcatca
     4021 ttgagaagcc ctggtatttc atcaaggaca cccagggcgt gattgggttt gtgggcgatc
     4081 cgccgatgcc gactccgtcc gaggaagtgg agtccatcaa ggcgcagatt tcggattccg
     4141 aagagcgcga gaagccgaag gtcaattttg aagtgggcga gaccatcaag atcaacaatg
     4201 gtccgttcct caatttcagc ggcgtgatcg aggaaatcga gccggaacgc gggaaattga
     4261 aggtcacggt gaatattttc ggacgaaaca cgccggtcga actcgaatac tggcaggtgg
     4321 agaaggcatg agaagcaaag acaatggagt tatggagtag tggagtgatg gaaaaatggg
     4381 aagaccccat tactccgtca ctccaatact ccactccccg attttatggc aaaaaaagtt
     4441 acaggtcaga ttaagttgca gattcctgcg ggccaggcga acccggcccc gccggtcggt
     4501 ccgtcgctcg gtcagcacgg cgtcaacatc atggcgttct gcaaggaatt taatgccgtg
     4561 accaaggctg ataccggttt ggtgattccg gtggtcatca cggtttatca ggacaagtcg
     4621 ttcactttca tcaccaagtc gccgccggca tccgtgctgt tgaagaaagc cgccggcatc
     4681 acctccggct cgaagacgcc gaacaaggaa aaggtcggca aggtgacgcg caaacaggtc
     4741 ttggacatcg tcaagaccaa gatgaaagat ttgaattcga ccagcgaaga agccggtttc
     4801 cgcgtcgttg ccgggacggc ccggagcatg ggcattgatg tcgtcggcta attttgaaca
     4861 aacaaccgcg ggagagcctc aaaactcgct tgcaccgcaa caaactgaat gccaaataaa
     4921 ccaagcaaaa gatacaaaaa agcactggaa caggtggatg gaaaacaatc ctatccgctg
     4981 aagtccgccg tggaagtgtt ggccaaattt ccgaaggcca agttcaacga aaccgtcgaa
     5041 gtcgcgttca agctcggggt tgatcccaag caatccgacc agatggtgcg cggcactgtt
     5101 ccgcttcctc acggcagcgg caagacggtg aaggtgctgg tttttgcccg caacggcgcg
     5161 gccgcggatg cagcgcgcgc agcaggcgcg gaatttgtcg gctacgaaga catgatcaaa
     5221 aaatgtgtcg aaggctggac tgatttcgat gtcgccatcg ccacgcccga agcgatggtc
     5281 gaggtgcgca agcttggcaa ggtgctcgga ccgcgcggtt tgatgcccaa tcccaagacc
     5341 ggcaccgtga ctgaggacac tgccaaggca gtgaaggaag tgaaagctgg ccgtgtggaa
     5401 ttcaagctgg acaaggccgg caacgtgcat gtgccgattg gcaaggcctc gttcactccg
     5461 gcccagatcg cggaaaacgc gcgtgtggtc atcgaagccg tcgcgaaagc acgtcccgcg
     5521 agcgtgaaag gctcttatct tcaaagttgc acactgtccg ccaccatgag tccgccggtg
     5581 cgcattgatg tgcgtgaatt cctggctgcc gcctaaacct caatttccag ataacatgcg
     5641 tgccgaaaaa caaaatctta cgaaggaata cttgacccgg ttgaacgcgt cgccgttctt
     5701 cattgtggtc aattatcagg gcctcaaagt gggtcccatc actgaattgc gcaaacggct
     5761 gaacagggcc ggttcagaaa ttcacgtggt caagaactcg attttccgcc ttgcggtcaa
     5821 ggaagccggc gtcggcgaac tgaacggcgc attggccgga cagatcgcgg tcatcaccgg
     5881 gcaaaaggaa gtttcagccg ccgcaaaggt cgtcaagaat ttcaatgcgg agttcgacaa
     5941 gctgaaaatc cgctttggtt atttgaacaa taaccgtttg gaagcgtcag acctccttgc
     6001 actggctgat ttgccgtcca tcgaagtctt gcgcagcaaa cttctggggg tgttgaaggc
     6061 cccgccgacc aagctggtgg cgttgctcaa cacgcccgcg agccagttgg cgcgggtgtt
     6121 gcaggcccgc gtggacaaag gcgcatgatc aatttcgggt tccggatggg acccaaagat
     6181 ttcgcctccg ttcaagtcga gcggcggtga aaaaacaaca aacaacaaag taaatcgtcg
     6241 gcttgcgggc tgtgagctta atgcatccgt ggccgcggat ggcgacgaga ctgaaaggta
     6301 acatggctga attagcatca ttggtagaac aactcggtaa attgaccgtg atcgaaaccg
     6361 ctgaactggt gaaacaactg gaaactgcct ggggcgtgac cgccgcagct cctgttgctg
     6421 tcgccgcggg tggtggtggt gcggcaggtg ctgctgccgc tccggctgaa gcgaagaccg
     6481 cttttgacgt gatcctcgcc tcggttccgg ccgacaagaa gatcgccgtc atcaaggccg
     6541 tgcgcgaagt caagactggc ctgggcctgg cggaagccaa ggcgctggtt gaaggcgctc
     6601 ccaagccggt gctcgaaggc gctcccaagg ccgaagctga cgccgccaag aaaaagctcg
     6661 aagaagctgg cgcgaaggtc gaggtcaagt aagcaggctt tacagggtgg cggggtgttc
     6721 ccgccaccat ttttcgtgct gtccgtcccg gttccggcag ccacttgcaa attgagccgg
     6781 aagaagcaga ttataagagc taaatttatc aaagccgaga gaagcggctt ttgtccggca
     6841 agtttcctgt tgaacacagg atgcttgcct tgtgtcgttt attgaaaaca aaagggctgg
     6901 cgttgttgcc agccatactt aaaaggtcca aatgccatcg cgagccactg aacgcatcaa
     6961 cttcgggaag atcaaagaaa tcatcgcccc gcccaacctg atcgaattgc aaaccaattc
     7021 gtacaaggaa ttcctccagg cggacatcgc gccctccaga cgcaagaatt tggggttgca
     7081 agcggtgttc acggaagtgt ttcccatcga gagctatgat ggcaagtgcg tgctggattt
     7141 tcacagctac gagatcggcg agcccaagct cgactggctc gaatgcctgc gcgaaggcct
     7201 gaccttcggc gcgccgcttt atgtcacctt cctgctcaag gaagaaaagg cgagcaagga
     7261 agaaaaggtg ttcatgggcg aattgcccct gatgacgccg caggggactt tcgtcatcaa
     7321 cggcgcggaa cgcgtcatcg tcagccagct ccatcgttcg cccggtctgg cgttcgagga
     7381 aacccagcat ccgaacggca agaagctgca atcgttccgc atcattcctg atcgcggttc
     7441 ctggtatgaa gcgcaatttg acaccagcga tttgctttac gtttatcttg accgcaagaa
     7501 acgccgccgc aaatttttga ccacgacttt cttccgcgct ctcggttacg gcacggacga
     7561 ggacatcctc aagctgttct atgagatcga ggaattgagc gtcaaggacg ccgaaaaact
     7621 ggaagacctt cagaacaagg tactcatcca ggacgcgacg gacaatgaca agggtgttgt
     7681 tgtggcccgc gcgttcgagc cgctttccaa ggcggtcatc aaacagatcg ccgaattggg
     7741 catcaacaag atcaaggtgg tggacaccac cgcggacgaa gggatcatca tcaagtgcat
     7801 gaagaaggat cccgcgaaga atgaagacga agcgttgaag gacatttatc gccgtttgcg
     7861 cccgggcgat ccgcccaccg ccgcgaacgc gcgcgccctc atcaagcgcc tgttctttga
     7921 tcccaagcgt tatgacttgg gccgcgttgg tcgttacaag atcaaccaga agctcggcat
     7981 caaaggcggc gattcccgca ttttgaccaa ggaagatttg gttgcggcga cgaagtatct
     8041 gctgcgcctt aaaaaaggcg aaggcagcct ggatgacatt gaccatctcg gcagccgccg
     8101 cgtccgcaca gttggtgaat tgctcgccaa ccagtgccgg gtcggtctgg cccgcacgga
     8161 acgtctcgtg aaagagcgca tgaccttgtt cgatcaaggc atggaaagca tggcgccgca
     8221 aaagctcatc aatcccaagg cgctctccgc cgtgatccgc gatttctttg gccgcagcca
     8281 attgtcgcaa ttcatggacc agatcaatcc gctcgccgaa ctgacccaca agcgccgtct
     8341 ttcggcactt gggcctgggg ggttgtcacg cgaccgcgcc ggtttcgaag tgcgcgacgt
     8401 tcatccgtcc cattacggcc gcatctgccc ggtggaaacg cccgaaggcc cgaacatcgg
     8461 tttgattgca tcgctcgcga ctttcgcgcg catcaacgat ttcggtttcc ttgaaacgcc
     8521 gtatcgcaag gtcgagcacg gccgcgtgac caatcacgtg gattatctca ccgcggaccg
     8581 cgaggaaact ttcgtggtcg cgcaggcgaa cgcaacgatt gacgacaagg gccacttcct
     8641 gaccgacaag gtcatgtgcc gcggcaaggg cgacttcatc gacgtcgaac ccgagcgcgt
     8701 ggattacatg gatgtttcgc cgaagcag
//
LOCUS       DASZMM010000520         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_522969, whole genome shotgun sequence.
ACCESSION   DASZMM010000520 DASZMM010000000
VERSION     DASZMM010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522969"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1506)
                     /locus_tag="VG754_12560"
     CDS             complement(<1..>1506)
                     /locus_tag="VG754_12560"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HWF20093.1"
                     /translation="GCELDPQLFAQQMARGVQDALSMDELTDAATLRMSQDYVREVVA
                     HEVGHVLGLRHNFAGSLEANLSRSDLDDWFRAYILGKPLDAYTNKIATSSVMDYNVFK
                     AAVFTGWRMRTVKEALPYDRGAIAWGYFDSPEVREKKMFFGTDQDVGRYGDVRRFDYG
                     TNPVVNAYAESAHLIEMLPNTIIETFIRARAPRNPHDRVPLEQVNLSYTMYANRIADE
                     LEDLLSWFRADARSLRVERKFDFIGDLNLKERHQAHWEYLTNQIEQLGGIDRAIFSFM
                     PVDLKLELKDKDKPTGIPVAERLSATNLTARLEKLLASTNYSTFVGLDDKKYSFTKDE
                     QELIIKRGKKCFEELEKEVIRQVCLRFTNASRNLGVEANGMVGDNDIVAALEKRIVDL
                     AKEVILAKDDSKRIKGTMDKAFVEVPEFKYDLATRMAAAHMLSPNIGSFKGWADETRG
                     DLNGDLKKEMESALNIDHFKDFKPSMLSRPVLEWYQEQQELLSQLPMPQPPP"
BASE COUNT          315 a          441 c          389 g          361 t
ORIGIN      
        1 ggcggcggtt gcggcatggg aagttgcgag agcaactcct gctgttcttg ataccactcg
       61 agcacgggac gcgacagcat cgaaggtttg aaatctttga aatgatcgat gttcaacgcg
      121 gattccattt ccttttttaa atcgccgttt aaatcgccgc gggtttcatc ggcccaacct
      181 ttgaacgagc cgatgttcgg gctgagcatg tgcgcggctg ccattcgtgt ggccaaatcg
      241 tatttgaatt cgggaacctc gacgaacgcc ttgtccatcg tgcctttgat gcgcttggaa
      301 tcgtccttcg caagaataac ttccttggcg agatcaacga tgcgcttttc cagcgccgcc
      361 acgatgtcat tgtcgccgac catgccgttc gcctccacgc cgaggttgcg cgacgcgttg
      421 gtgaatcgga gacagacttg gcgtattact tccttttcca attcctcaaa acattttttc
      481 ccccgcttga tgatcaattc ctgttcatcc ttggtgaaac tgtatttctt gtcatcaagg
      541 ccgacgaacg tggagtaatt ggtcgaagcc aaaagctttt ccaatcgcgc cgtgagattg
      601 gtcgcgctga gacgttcggc gactggaatg cccgtcggtt tgtccttgtc cttcaattcc
      661 aatttcaaat ccaccggcat gaatgaaaat atcgcgcgat caatgccgcc aagttgctca
      721 atctggttcg tgagatattc ccaatgcgcc tgatggcgtt ccttcaaatt aaggtcgccg
      781 atgaaatcaa acttcctttc cacccgcaac gaacgggcgt ccgcgcgaaa ccacgaaagc
      841 aggtcttcca gttcgtccgc gatcctgttc gcgtacatcg tgtagctgag attgacctgt
      901 tcgagcggca cgcggtcatg tggattgcgc ggcgcgcggg cgcggatgaa cgtctcgatg
      961 atcgtgttcg gcagcatctc gatcaaatgc gcgctttccg cgtaagcgtt gacgacagga
     1021 ttggtgccat aatcaaaccg ccgcacgtcg ccgtaacgac ccacgtcctg gtcggtgccg
     1081 aagaacatct ttttctcgcg cacttccggg ctgtcgaaat aaccccacgc gatggccccg
     1141 cgatcatacg gcaacgcttc cttgaccgtg cgcatccgcc agccggtgaa caccgcggcc
     1201 ttgaacacgt tgtaatccat caccgaactc gtcgcgattt tgtttgtgta agcgtccagc
     1261 ggcttgccga ggatgtaagc gcggaaccaa tcatccaaat cgctccggct gaggttcgct
     1321 tcaaggctgc cggcaaaatt atgccgcaag ccgagcacat ggccaacttc atgcgccaca
     1381 acttcgcgca cataatcctg cgacatgcgc aaggtcgccg catcagtcaa ttcatccatc
     1441 gagagcgcgt cctgcacgcc gcgcgccatc tgctgcgcga aaagttgcgg gtcgagctcg
     1501 catccc
//
LOCUS       DASZMM010000521         8576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2425989, whole genome shotgun sequence.
ACCESSION   DASZMM010000521 DASZMM010000000
VERSION     DASZMM010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425989"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VG754_12565"
     CDS             complement(<1..203)
                     /locus_tag="VG754_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20094.1"
                     /translation="MSRNAQTDASRRCRIGRLLCFCLFLALLSQAQAQAPRALFGTNS
                     PNARVVIVEDPYVVENLKPVPERV"
     gene            234..2060
                     /locus_tag="VG754_12570"
     CDS             234..2060
                     /locus_tag="VG754_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF20095.1"
                     /translation="MQTNKKRLHEWIHIFLLAAVVAWLAGCTPAGPRALLRGKKLIEQ
                     GDYADAVDELKIATSLLSTNAQAWNYLGLAYHHAGQPAHAVQAYQRALKLNHDLVVVH
                     YNLGCLWLEQNRPETLEGARDELTAFVLHQPNSSDGWLKLGTTQLRLGELPQAEASFK
                     EVQRLNPQNAEALNDLGVIQMQRRRNRDAAAYFNAALKLQPNYGPALLNLAVTAGYLN
                     NPSLALQNYQQYLQLNPRPANWDAVNVIAKQLEQQLNPAPVLPSNTAVAANPVTNIQN
                     HPVTAPTNVPKPETVASAPHPVMPPPKPAISTDTTDTAETTVKPEVVRLPDSPPIQAA
                     GQGNRNLAPVASSSINTSDVQIIGDPGEARENSVPAKRGFFQKLNPMGLFHHTTKPAD
                     KPVPPAPAVTGADVVEGPPIVSTDLTPAPKLPAPAARPISIPRYPYLSPAKPPAGNRA
                     KAEQLVAKAAEARRDDRLEDAVGLYRAATQADPSYFEAQSGLGLAAFDSGNLPESLRA
                     YEMALAITPDSFNARFNFGLALKRANYIRDAAQELERLLASNSAGESSVHLAMVHLTL
                     ANLYAEQFHQTASARAHYLKVLELDPHNGQATSIRYWLQDNG"
     gene            2241..2864
                     /locus_tag="VG754_12575"
     CDS             2241..2864
                     /locus_tag="VG754_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWF20096.1"
                     /translation="MHPKDFAHIPLPNYFDALVLAVLLWGLNKGRKHGMSEELMMTLQ
                     WIVIVVAGAFLYRPFGDLLATTTPVSHLFCYITIYIVAAIITKIIFGMVKKGIGGKLV
                     GSDVFGAGEYYLGMVAGAIRYACMIMAALALLNAPYYSPQEIASRKAYENDVYGNSFF
                     PGIPGIQQNVFQESLIGSLIKQRAGVLLIASTKTENVALKRRKDDLP"
     gene            2920..4773
                     /gene="dnaG"
                     /locus_tag="VG754_12580"
     CDS             2920..4773
                     /gene="dnaG"
                     /locus_tag="VG754_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWF20097.1"
                     /translation="MAGFFSDDTIEQIRAASDIVDVIGSYVPLKRAGANFTALCPFHR
                     EKSPSFNVNPQRQIFHCFGCHKGGDVFTFVKEFENIPFPDAVRRLAERAKIPLQLDAT
                     PGQKQNRHIKDTLLQIHEQITQRWQSALANEASGQVARDYLAKRGVSAEAMKLFRLGY
                     APDLWDDTVNWAKSKGHELAIVEQGGLILAKEGGGHYDRFRGRLMFPICDEQGRVIGF
                     SGRVLDAEAKTAKYVNSPETPIFTKGKVFFGLDKSKRALLDADSAIVCEGQLDLIACY
                     MAGVQNIVAPQGTALTAEHARILKRYVDEVVLCFDSDNAGQNAAVRSLDSLLASGVAI
                     RVAVVPAPHDPDSFIKEFGGEAFKQLIAKAEGFFDYYLNRLCATNDLATDKGRLAVLQ
                     SMAEAVHKTGNSVLVDKYAQKTALRLGVTPEAVRSDFKKAARLKRATPVPAEPESPDA
                     PTASETAGATSADPLPKPSTPEYWLLKLSLLHEDSLEWTQAHLDLAWVQHAQVRDLLS
                     RRLELNVNAGWQGVAPFLTQCESDEMRSLLIEATMDERPIANAAQQIADITLRLRNQF
                     IDRQLSALTQRSAQPELNDEQRLEILHQQQALRQLKRQPLTPLPGSVEEPF"
     gene            4958..5974
                     /locus_tag="VG754_12585"
     CDS             4958..5974
                     /locus_tag="VG754_12585"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HWF20098.1"
                     /translation="MRRAFLCVLTLGISGATLMRAGADTVDFVKDIQPIFQESCLKCH
                     GPEKQKGDLRLDSKAAAFKGGKDGQVIVPQQADKSDLYRRIILPEGSDDVMPSKGALL
                     TKKQTDLIKDWINQGAAWPEGVIAAAKPDETPGPSPNAVFAALTPAKPTAAETGAISK
                     LAASGIDARLIAVNLPWRGASFRALGTNANDAAIAPVKDILSLVELNLAGTKITDAGL
                     QNLAGLTNLLALHLELTGVTDAGLAHLKHLDHLTYLNLYGTSVTDKGLEQVKNLHNLK
                     HLYLWQTKVSADGATDLQKAIPGVVISRGWENEPAAQKVESKEMDKKESKEPAAADKK
                     EAKK"
     gene            complement(6105..6422)
                     /locus_tag="VG754_12590"
     CDS             complement(6105..6422)
                     /locus_tag="VG754_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20099.1"
                     /translation="MLGIATLASGCVVRERVVYSPPLNGAPPPGGEVVVNEPPPAPLV
                     ESYTVAPDPTFVWVGGVWIWRGGWIWEPGHWAHPPRPGAVWIGPRYVFRGGRHVWVRG
                     YWR"
     gene            complement(6601..7407)
                     /gene="thiD"
                     /locus_tag="VG754_12595"
     CDS             complement(6601..7407)
                     /gene="thiD"
                     /locus_tag="VG754_12595"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWF20100.1"
                     /translation="MPKEAQVPVALSIAGSDSGGGAGIQADLKTFAALGVHGASAITC
                     ITAQNPARVTGIHASPPAMVRRQIEVVFAELPPAAIKTGMLFSAEIIRAVVGALKKNG
                     KRPALVVDPVMISTSGKPLLKSSAVKILKRELLPLATLVTPNLDEAARLTGRRLHSVE
                     EMRVAAKKIFDEFGCAALIKGGHLRGTVEATDIFFDGETELLLTAPFVKGVRTHGTGC
                     TYSAAITAFLALGQPLPKAIASAKEYVTAAIGGHYFAARHPVLEHFKFDF"
     gene            7591..8115
                     /locus_tag="VG754_12600"
     CDS             7591..8115
                     /locus_tag="VG754_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20101.1"
                     /translation="MAIGQIPRFGKPERNLPVDFPAFTAFSRLFSPFFVGGGGLPQVQ
                     GSKFKIPILYPPSSFRAGVSLGVWFVVKASAFSPILRHSNTPFTCRESSLCQTMQKTR
                     QSTFHQCITSFLKKSSQGESSYYLQRVKARPIGKSAEDWEQLHPGAWQMVRYLLEMQI
                     MTSRINDPTPFHLT"
     gene            8172..>8576
                     /locus_tag="VG754_12605"
     CDS             8172..>8576
                     /locus_tag="VG754_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20102.1"
                     /translation="MSATSSANEVTGARGRSGATWIAEAFSIFLLAAMAVYFLAISWR
                     KWPDPLVDSGPQLYAIWQVSEGARLYHDFLWNYGPFSVCLNAMLFKVFGPGLMVLVTT
                     NLVFYALILVLAYLAFRMAWGRLGAFAALAVFI"
BASE COUNT         1977 a         2492 c         2242 g         1865 t
ORIGIN      
        1 acgcgctcgg gcaccggctt gagattttcg acgacataag gatcttcgac gatcacgacg
       61 cgggcattgg gcgaattcgt tccgaaaagc gcacgcggcg cctgtgcttg cgcttgcgag
      121 agcaaggcga ggaacaggca aaagcaaagc agccgcccaa tcctgcatcg gcggctcgcg
      181 tcggtctgtg cgtttcttga cacttctggc ggcggatgtt accaccagat gagatgcaga
      241 ccaacaaaaa gcggttacac gagtggattc atatttttct gcttgccgcc gtggttgcct
      301 ggcttgcggg atgcactccc gcgggtccgc gcgcgctttt gcgcgggaaa aaacttattg
      361 agcaaggcga ttacgccgac gccgtggacg aactcaagat tgccacttcg ctgctctcga
      421 cgaacgcgca ggcgtggaat tatctcggtc ttgcttatca ccatgcaggc cagccggccc
      481 atgccgtgca agcatatcaa cgcgcattga aactaaatca tgatctggtc gtggtgcatt
      541 acaaccttgg ctgcctttgg ttggagcaaa accgccctga gacgttggag ggcgcgcgcg
      601 acgaattgac tgcttttgtt ttgcatcagc ccaattcttc ggatggctgg ctgaagctcg
      661 gcacgacgca attgcggctt ggcgaactgc cgcaggctga ggcgagtttc aaggaagtgc
      721 aacggctcaa cccgcaaaac gccgaggctt tgaacgactt gggcgtgatt caaatgcagc
      781 gcaggcgcaa ccgcgacgca gccgcatatt tcaatgccgc gttgaagctt cagccgaatt
      841 atggcccggc attattgaat ctcgccgtca ccgcaggtta tctgaacaat ccctcgctcg
      901 ccttgcaaaa ttaccagcaa tatctgcaac tcaatccgcg cccggcgaat tgggacgccg
      961 tcaacgtcat cgcaaagcag ctcgaacagc aacttaatcc tgcgccggtt ttgcccagca
     1021 ataccgcagt cgccgcaaat cctgtcacaa acattcaaaa ccatcctgtc accgcgccaa
     1081 cgaatgttcc caaaccggaa actgtcgcca gcgcgccgca tccggttatg ccgccgccga
     1141 agcccgcaat ttccacggac accactgaca ccgctgaaac cacagtgaaa ccggaagttg
     1201 tgcgactgcc ggattcgccg cctatccaag ccgccgggca gggcaaccga aatctcgcgc
     1261 cagtcgcgtc ctcctcgatc aacacatctg acgtgcagat aattggtgat ccgggggaag
     1321 cgcgggaaaa ttctgtgccg gcgaagcgcg gctttttcca gaagttgaac ccgatgggtt
     1381 tgtttcacca cacgacaaag ccagccgata aacccgttcc gcccgctcca gcagtgactg
     1441 gtgctgacgt agtggaaggt ccgcccatcg tgtccaccga tcttactcca gcgccaaaac
     1501 ttccggctcc tgcggcgcgt cccatttcca taccgcgcta tccgtatctg tcgcccgcca
     1561 agccgcctgc gggaaatcgc gcaaaggcgg aacaattggt cgcgaaagca gcggaagcgc
     1621 gacgcgatga ccggcttgaa gacgcggtcg gcctctaccg cgcggcgacg caagccgacc
     1681 cgtcctattt cgaggcgcaa tccggcctgg gattggccgc attcgattcg ggcaatttgc
     1741 cggaatcgtt gcgggcttac gaaatggcgc tggccatcac cccggattct ttcaacgcgc
     1801 gttttaattt cggcctcgcg ctcaaacggg ccaattatat tcgtgacgcc gcgcaggaat
     1861 tggaacgcct gcttgcctcc aattccgccg gtgaatcttc cgtccatctg gcgatggttc
     1921 acctcacgct cgcgaatctt tacgccgaac aattccatca aacagcatct gcgcgcgccc
     1981 actatttgaa agtcttggaa ttggatcccc acaacgggca agcgacctcc atacgttatt
     2041 ggcttcaaga caacgggtga gcggaaattc ttatcgatag acaccaattt cattgattag
     2101 cccaagcttg aatcggcaat ggtgacaatt cgtgcaattc attgtctttt atgcgcagtt
     2161 cttcgcactc cgaattttgg ccgacaactt gcttatatac cttggaaact tgttaaaagt
     2221 gtccaaaaat tgagcatttt atgcacccaa aagactttgc gcacattccg ctgccgaatt
     2281 atttcgatgc gttggtgttg gctgtgctct tgtggggcct caacaagggc cgcaagcatg
     2341 gcatgtccga agaactgatg atgaccttgc aatggattgt catcgtggtt gccggggcgt
     2401 ttctttaccg gccgtttggc gacttgctgg ccacgaccac gccggtcagc catctctttt
     2461 gttacatcac aatttatatt gttgcggcga tcatcacgaa aatcatcttc gggatggtca
     2521 agaaaggcat cggcggaaaa ctggtcggaa gcgatgtctt tggcgccggg gaatattacc
     2581 tgggcatggt cgccggggcc attcgttacg cctgcatgat catggcggca ttggcccttt
     2641 tgaatgctcc gtattacagc ccgcaagaga ttgcctcgcg caaggcctac gaaaacgacg
     2701 tctatggcaa cagcttcttt cccggcattc ccggcattca gcaaaatgtt ttccaggaat
     2761 cattgatcgg cagtttgatc aagcaacgtg cgggcgttct cctcatcgcc tccacgaaaa
     2821 ccgaaaacgt cgccctcaaa cgtcgaaaag acgatttgcc ttaatcccgg cggcgcgtta
     2881 gaacagtcat ggcgtagaaa ccgcggttgg tttcgtctca tggctggatt tttttcggat
     2941 gacacaatcg agcagatacg tgcggccagt gacatcgtgg atgtcatcgg ctcctacgtg
     3001 ccgttgaaac gcgccggcgc gaacttcacc gcgctctgcc cttttcaccg cgaaaaaagc
     3061 cccagcttca acgtcaatcc gcaacgccaa atttttcatt gcttcggctg ccacaagggc
     3121 ggcgatgtct tcaccttcgt caaggaattc gagaacattc cttttcccga cgccgtccga
     3181 cgactcgctg aacgcgcaaa gataccgctt caactcgacg ccactcccgg ccagaaacaa
     3241 aatcgccata tcaaggacac gctgctccag atccacgagc agatcacgca acgctggcaa
     3301 agcgcgctgg ccaacgaagc ctccggtcag gtcgcgcgcg attatctcgc caagcgcggc
     3361 gtctccgccg aggccatgaa attgttccga ctcggctacg cgccggacct ttgggacgac
     3421 acggtcaatt gggccaaaag taaaggacac gaactcgcga ttgtcgagca gggcgggctt
     3481 attttggcaa aggaaggcgg cggacattac gaccgttttc gcgggcgctt gatgtttccg
     3541 atttgcgacg agcagggcag ggtgatcggt ttcagcgggc gcgttctcga tgccgaagcc
     3601 aagaccgcca aatacgtcaa ctcacccgag acgccgattt tcacgaaagg caaagttttt
     3661 ttcggcctcg acaaatcgaa acgcgctttg ctcgatgccg attccgccat cgtgtgcgaa
     3721 gggcagcttg accttatcgc ctgttacatg gccggtgtgc aaaacatcgt cgcgccgcag
     3781 ggaacggcct tgaccgccga gcacgcgcgc atcctcaagc gttacgtgga tgaagtcgtg
     3841 ctctgcttcg attccgacaa cgccggccag aacgccgccg tccgttccct cgatagtttg
     3901 ctggcatcgg gagtggcaat ccgcgttgcg gtggttcccg ccccgcatga ccctgacagt
     3961 ttcatcaagg aatttggcgg ggaagcgttc aagcaactca tcgcaaaagc ggaaggtttt
     4021 tttgattatt acctcaatcg cctctgcgcc acgaacgacc tcgcgaccga caaaggccgc
     4081 ctcgcggtgt tgcaatcaat ggccgaagcc gttcacaaaa ccggtaattc agttctcgtg
     4141 gacaaatacg cgcaaaaaac cgcgttgcga ctcggcgtca cgcccgaagc agtccgaagt
     4201 gacttcaaaa aagccgcgcg attaaaacgc gccacgccgg tccccgctga acctgaaagc
     4261 ccagacgcac caaccgcttc cgaaacagcc ggcgcaactt cggcagaccc actgccaaag
     4321 cccagcacgc ctgaatattg gctcctgaaa ttatcgctct tgcatgaaga ttcgcttgaa
     4381 tggacgcaag cccatctcga ccttgcctgg gtgcagcacg cgcaggtgcg tgacctcttg
     4441 agtcgtcggt tggaattgaa cgtgaatgca ggctggcagg gcgtcgctcc ttttctcacc
     4501 caatgcgaat ccgacgaaat gcgctcattg ctcattgaag cgacaatgga cgaacgtccc
     4561 attgcgaacg ccgcgcaaca aatcgccgac atcaccttgc gcctgcgcaa tcaattcatt
     4621 gaccgccaac tcagcgcatt gacccaacgc tccgcccagc ctgaactcaa tgacgaacaa
     4681 cgtctcgaaa ttcttcacca gcaacaagca cttcgacaac tcaaacgcca gcccctgacg
     4741 ccattgccgg gaagcgtgga agagccgttt tgaaaaatcc gttggcagcg cgcgtccact
     4801 ttgtacccat gcttcagcat gtcctcacac cggcgcgtga aacgcggctc aagccgaaac
     4861 gttgcttcca cccctcgatg cgtcaattga agccggcatt gcatcccgaa acgtttgctg
     4921 tcataattcg cgtcactcag gcaacaagta aaattttatg cgaagagctt ttttgtgtgt
     4981 gttaacgctg ggaatatccg gcgcgacatt gatgcgcgcg ggcgctgata cggtggattt
     5041 cgtgaaggac atccagccca tttttcagga gtcctgcctc aaatgtcacg gccccgaaaa
     5101 acaaaaaggc gacttgcgcc tcgattcgaa ggcggcggct ttcaaaggcg gcaaggacgg
     5161 gcaggtgatc gttccccaac aggcggacaa gagcgatttg tatcgccgca tcattttgcc
     5221 ggagggaagc gacgacgtca tgccgagcaa gggcgcactg ctcacaaaaa agcaaaccga
     5281 cctgatcaag gactggatca atcaaggcgc ggcgtggccc gagggggtca ttgccgcagc
     5341 gaaaccggat gaaactcctg gcccatcgcc caacgccgtt ttcgccgcgt taacgcccgc
     5401 gaaaccgacc gctgccgaaa ccggcgcaat atccaaattg gccgccagcg gaattgacgc
     5461 ccggctcatc gcggtgaatt tgccctggcg cggggcgagt tttcgcgcat tgggcactaa
     5521 tgccaacgac gcagccatcg ccccggtgaa ggacattctc agcctcgtgg aattgaatct
     5581 ggccggcacg aaaatcaccg atgccgggct tcagaatctt gccgggctga cgaacctcct
     5641 cgcgctgcat ctggaattga ccggcgtcac tgacgcgggc ctcgcgcatc tgaaacatct
     5701 ggatcatctc acttacctca acctttacgg aacttccgtg accgacaaag gcctggaaca
     5761 agttaaaaat ttgcacaatt tgaaacatct ttatctgtgg caaaccaagg tcagcgcgga
     5821 tggcgcgacc gatttgcaga aggcaattcc cggcgtggtg atttcacgcg gatgggaaaa
     5881 cgaacccgct gcgcaaaaag tcgaatcgaa agagatggac aagaaagaat cgaaggagcc
     5941 cgcagccgcg gacaaaaagg aagcgaagaa gtaagctcca tcaatatctg agcgccaaaa
     6001 aaattttcac ctcaaaaaga taaagctcca atccttttca gattggagct ttatctttga
     6061 ctattctctg gagcttggag attggaattt ggagcttggt taaatcatct ccaataaccg
     6121 cggacccaaa cgtggcgtcc gccgcgaaag acatagcgag ggccgatcca aaccgcgccg
     6181 ggacgaggcg ggtgcgccca atggcccggt tcccaaatcc agcctccacg ccaaatccat
     6241 acaccgccga cccacacgaa agtagggtca ggcgcaaccg tatagctttc gaccaagggc
     6301 gcaggcggcg gttcgttcac gaccacttca cctccgggcg gaggcgcgcc attgagaggc
     6361 ggactgtaaa cgacccgttc acgcaccacg caaccgctcg cgagagtcgc aatgccaagc
     6421 attagaactg ctgctttcaa taatgatgtt tgtttcatat cgttaatgag acgcgcaagg
     6481 ccgggtgtta ttcattcgtc gtttgcatct ttccatcgtc ttcggccact gctgtttagg
     6541 catcgtgcgc atctggttat cagaccggat gaccctgaaa aatgttacag tcaaatgcga
     6601 ttagaaatca aatttgaaat gctcaagcac cggatgcctt gcggcaaaat aatgtcctcc
     6661 aatggccgcc gtcacatatt ccttggcact cgcgattgcc ttcggcaaag gctggcccaa
     6721 cgcaaggaac gcggtgatcg ccgccgaata cgtgcagccg gtcccgtgag tgcgcacgcc
     6781 tttcacaaat ggcgcggtca acagtaattc cgtttcacca tcgaagaaaa tgtccgtggc
     6841 ttcaaccgtg cctcgaagat gaccgccttt gatcaacgcg gcgcaaccga actcatcaaa
     6901 aatctttttc gccgcaacgc gcatctcttc caccgaatgc aatcgccgtc ccgtgagtcg
     6961 cgccgcttca tcaagattgg gcgtgaccaa agttgccaga ggcagcagct cgcgtttgag
     7021 aattttgacg gcggaagatt tgagcaacgg ttttccgctg gtcgaaatca tcaccgggtc
     7081 cacgaccagc gcgggacgct tcccattttt tttgagagcg ccaaccaccg cgcgaatgat
     7141 ttcggcggaa aaaagcattc cggttttgat tgcggcaggc ggcaattcgg cgaacaccac
     7201 ttcgatttgc cggcgcacca tcgcgggcgg agatgcatgt atccctgtca cgcgcgccgg
     7261 attttgcgcc gtgatgcagg tgatggcgct ggcgccatgc acaccaagcg cggcgaacgt
     7321 tttcaggtcc gcctggattc ccgccccacc gccgctgtcc gatcccgcga tgctcagcgc
     7381 gaccggaacc tgtgcttcct ttggcatgaa aatattatgg cgagtttcca tgcattttcc
     7441 cacagttttg tgtccggctt gagaagcttt gaaaaacttt tttgtgccca ttggatcgaa
     7501 aatgatcgac aaccctcctt cttcctgaaa tactgacctt cgcgtgaacc aaggaacgca
     7561 aaagaggacg ccatcaggtg tccaagatga gtggcgattg gccaaatccc aagatttggc
     7621 aaaccagagc gaaatttacc cgttgatttc ccggctttta ccgccttttc acgcctcttt
     7681 tcgccttttt ttgtgggggg aggggggtta ccccaagttc aaggttcaaa gttcaaaatt
     7741 cccatcctct atcctccatc ctcatttcgg gcgggggtgt cgcttggcgt ttggttcgtg
     7801 gtcaaggcaa gcgccttttc accaatactc cgccactcca acactccatt tacgtgccgc
     7861 gagtcaagcc tttgtcaaac catgcaaaaa acaagacaat cgactttcca tcaatgcatt
     7921 acttcatttt taaagaaatc gagtcaaggc gagtcaagct attatttgca acgagtcaag
     7981 gcacgaccaa ttggcaaaag cgcagaggac tgggaacaac ttcacccagg tgcttggcag
     8041 atggtgcgct atttgcttga aatgcaaatt atgacatcac gcattaatga tcccacgccg
     8101 tttcacttga cctgaaaatc atcgccttta cgatgacaac cgcttgcaac aaaagcatcg
     8161 catcggatcc tatgagcgcc acttcatccg caaatgaagt gaccggcgcg cgcgggcggt
     8221 ctggagcaac ttggattgcc gaagcgttct cgatttttct gctggccgcg atggccgttt
     8281 attttctggc gatttcctgg cgcaaatggc ccgacccgct cgttgattct ggcccgcaat
     8341 tgtacgccat ctggcaggtg tcggaaggag cgcggctgta tcacgatttt ctttggaatt
     8401 acgggccgtt ctcggtctgc cttaacgcga tgctgttcaa agttttcggg ccgggattga
     8461 tggtgctggt gacgacgaac cttgtttttt acgcgttgat ccttgtgctg gcgtatctcg
     8521 cattccggat ggcgtggggg cgactgggag cgtttgcggc tttggcggtt ttcatt
//
LOCUS       DASZMM010000522         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4358061, whole genome shotgun sequence.
ACCESSION   DASZMM010000522 DASZMM010000000
VERSION     DASZMM010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4358061"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="cysC"
                     /locus_tag="VG754_12610"
     CDS             <1..1248
                     /gene="cysC"
                     /locus_tag="VG754_12610"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWF20103.1"
                     /translation="SERPANQPLRFPVQDIYRFDERRILAGRVEAGSIKVGDKLLFSP
                     GNKTSTVKTIERWNAPARPDAAAGESIGITLTEQIFVERGAVAALETASPYELSRFKA
                     RLFWLGHKPFSKGKLYKLKLATQQVDCEIETIEKIIDASTLETISRAPQEIFVGRHEV
                     AELTIRTKRPVAFDTHSEIVPMGRFVIVDEFEVSGGGIVAEDNYPKRTADSLHKSDNI
                     FWSKSKITSSQRSMRNRHAGCVVWLTGLSGAGKSTIATELERELFNAGKSAYVLDGDK
                     IRHGLCSDLAFSPEDRKENIRRVGEVAKLFADAGIICVTAFISPYRADRELVRKMVKG
                     DQFIEVFVNAPLAVCEQRDPKGLYVKARANQIKEFTGISAPYEVPPNPELELHTDKLS
                     TAECVARIMEFLHAEQEDTEISI"
BASE COUNT          388 a          444 c          423 g          348 t
ORIGIN      
        1 tccgaacggc ccgcaaacca gccgttgcgc tttcccgtcc aggatattta tcgctttgac
       61 gaacggcgta ttctcgcagg ccgtgttgag gccggctcca tcaaggtcgg cgacaaattg
      121 ttgttcagtc ccggcaacaa aaccagcacg gtaaaaacca tcgaacgctg gaatgcgcca
      181 gcgcggccag acgctgccgc gggcgaatcc atcggcatca cgcttaccga acagattttt
      241 gtcgaacgcg gcgcggtggc tgcgttggaa accgcgtcgc cttacgaatt gagccggttc
      301 aaggcgcggc tgttttggct cggtcacaag cccttttcca agggaaaact ctacaaactc
      361 aagctcgcca cccaacaggt ggattgcgaa atcgagacga ttgaaaaaat tattgatgcc
      421 tccacgctcg aaacgatttc gcgcgcgccg caggaaattt ttgtcggacg acatgaagtc
      481 gcggaattga ccatccgcac caaacgcccg gttgccttcg acacgcattc ggaaatcgtg
      541 ccgatgggtc ggttcgtcat tgtggatgaa tttgaagtct ccggcggggg catcgtcgcc
      601 gaggacaatt atcccaagcg caccgccgac agcctgcaca agagcgacaa cattttctgg
      661 agcaagagca agatcacgag cagccagcgt tccatgcgca atcgccatgc cggttgcgtg
      721 gtgtggctga ccggactttc cggcgcgggc aaatccacca tcgcaaccga actcgaacgc
      781 gaattgttca acgctggcaa gagcgcttac gtccttgacg gcgacaaaat ccgccacggt
      841 ctttgttccg acctcgcgtt ttcgccggaa gaccgcaagg aaaacatccg gcgcgtgggt
      901 gaagttgcca agctttttgc cgacgccggc atcatctgcg tcacggcctt catttcgcca
      961 tatcgcgccg accgcgagct ggttcgcaag atggtgaaag gcgaccagtt tatcgaggtg
     1021 tttgtgaacg cgccgctggc ggtttgcgag cagcgcgatc ccaagggcct ttacgtcaag
     1081 gcccgcgcga accaaatcaa ggaattcacc ggcatttctg ctccctacga agtcccgccc
     1141 aatcccgaac tcgaattgca caccgacaaa ctttccaccg ccgaatgtgt cgcccgcatc
     1201 atggaattct tgcacgctga gcaggaggac acagaaattt cgatttaaat ttttttggtt
     1261 ccagaaaggt tttaatgctt tgcaagaatc agatgattca ttcgaacatt gaaaagatga
     1321 accatcgtaa taaatctctg gaaggatgaa gcgaaacacg ttggtttgga ttggcattat
     1381 cgcacttacg acagcaatcg ggtgccggag gtccgttcag tcgtcaggcg gaatggctcc
     1441 tacagtaacc aatggcgagc aattaacaat taactatctt gcatataatg tcacaagccc
     1501 gcaccggtgg gatgttgtcg cccttgaaca gccgtcattt actaatttag tggtggtaaa
     1561 gagggtcttc gctctgccaa gtgaaacgat ttcactgaca acg
//
LOCUS       DASZMM010000523         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5167465, whole genome shotgun sequence.
ACCESSION   DASZMM010000523 DASZMM010000000
VERSION     DASZMM010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5167465"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VG754_12615"
     CDS             <1..873
                     /locus_tag="VG754_12615"
                     /inference="COORDINATES: protein motif:HMM:NF045341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HWF20104.1"
                     /translation="YLAVAPANSPENHFLLPDGRDAAISLGTTVHAQMVRYLFTACIE
                     SSKILDVDEEFRKELIAKRARLSPTPIGTDGRVMEWPEEYKESEPHHRHVSHLWGLYP
                     GDEISPDASPEFAKAARKTLEARSDNGVGWSLAYKAALWARLQDGDHAWLLVRKALQP
                     AQGMEIRYDDGGGVYPNLFDASPPFQIDGNFGVTAAIAEMLLQSQEGPIELLPALPKA
                     WKNGKVTGLRARGGFTVDIAWTNGRLSAATIKADKSGTCRVEYAGKTIDLKIHAGGSV
                     HLGADLKKFKRFCG"
     gene            complement(854..1864)
                     /locus_tag="VG754_12620"
     CDS             complement(854..1864)
                     /locus_tag="VG754_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWF20105.1"
                     /translation="MSTRPVYHARRQRFGIDRCEPQTRAVREGKIEFHALTKGHYPGM
                     PVPKNILPGLNSIGFWSAGGAQDWGEDPHRNEGIEIVFLETGSMVFTLGEKHHDLRAG
                     QFIITRPWQLHKLGAPNIGPGRLHWLIIDVGVRRPHQNWNWPDWVVLTKNDLAELTRR
                     LRHNERPVWMSTPAIAQSFRELAHSIVKWDQAHSVSRLAVSLNQLLIGILDVFSKERK
                     EGDPALVSHRRTVELFLRDLEQRRIDLSEPWTLDRMAEQCGMGVTAFSKYTRQIVNAG
                     PIEFLNACRLDLAREKLNANGKHSITEIAFACGFNSSQYFATRFRQRFQTSPSQFIHK
                     SA"
BASE COUNT          447 a          537 c          612 g          445 t
ORIGIN      
        1 tatctcgccg tggccccggc gaattcgccg gaaaaccatt ttcttttgcc tgatggacgg
       61 gacgcggcga tttcattggg cacgacggtt catgcccaaa tggtgcgcta tctttttaca
      121 gcatgcattg aatcatcaaa aattctcgat gttgatgagg aatttcgcaa ggagttgatc
      181 gcaaaacgcg cgcggctttc gcccacgccc atcggcacgg acggacgcgt gatggaatgg
      241 cccgaagaat acaaggaatc ggaaccgcat catcgacacg tctcgcacct ctggggactg
      301 tatcccgggg atgaaatttc gcccgatgcc agtcccgaat ttgcgaaggc agcgcgcaaa
      361 actttggaag cgcggagcga taatggagtg ggctggtccc tcgcttacaa agcggcgctg
      421 tgggcgcgac ttcaggacgg agaccacgcg tggctgctcg tccgcaaggc attgcaaccg
      481 gcgcagggaa tggagatacg ctacgatgat ggcggcggag tttacccgaa tctatttgat
      541 gcttctccgc cgtttcaaat tgacggcaat tttggcgtga ctgcggccat cgctgaaatg
      601 ctgctgcaaa gccaggaggg gccgattgaa ctgctgcccg cattgccgaa ggcgtggaaa
      661 aatggaaagg tgaccggttt gcgcgcacgc ggcggattta ccgtggacat tgcttggacc
      721 aatggccggt tgagtgcggc aaccatcaag gccgacaaat cgggaacttg tcgggtggaa
      781 tatgcaggca agacgattga cctgaaaatt cacgcgggag gcagcgtcca tctcggcgcg
      841 gatctcaaaa agtttaagcg cttttgtgga tgaactggct gggtgaggtt tggaaacgct
      901 gacgaaaacg ggtggcaaaa tactggctgg aattgaaacc gcaggcgaat gcgatttcgg
      961 tgatggaatg cttgccgttt gcgttcaact tttcccgggc caaatcgaga cggcaggcgt
     1021 tcaggaattc aatcgggccg gcgttcacaa tttggcgcgt atattttgag aaggcagtca
     1081 cacccatgcc gcattgttcg gccatgcggt caagcgtcca cggctcgctc aggtcgatgc
     1141 gccgttgctc aaggtcgcgc aaaaataatt cgactgtgcg gcgatgcgag accagggcgg
     1201 gatcgccctc tttccgttcc ttcgaaaaca catcgaggat gccgatgagc aactggttca
     1261 aactcaccgc aaggcgggag acggagtgcg cctggtccca cttcactatc gaatgcgcca
     1321 gttcgcggaa ggattgcgcg atggcgggcg tggacatcca aaccggcctt tcgttgtgcc
     1381 tcagccggcg cgtcaattca gccagatcat tttttgtcag cacgacccaa tccggccagt
     1441 tccaattttg atgcggccgg cggacgccca catcaatgat cagccaatgc aaacgacccg
     1501 ggccgatgtt gggagcgccc agcttatgaa gctgccacgg ccgggtgatg atgaactggc
     1561 cggcgcgcag gtcatgatgt ttttcgccga gtgtgaacac catggaaccg gtttccagga
     1621 aaacgatttc aatgccttcg ttgcggtgcg ggtcttcgcc ccaatcttgc gccccgccag
     1681 cgctccagaa tccgatgctg ttcaacccgg gaagaatgtt ttttggaaca ggcatgccgg
     1741 gataatgacc tttggtcaac gcgtgaaatt ctattttgcc ttcacggaca gcccgcgtct
     1801 gcggttcgca acggtcaatc ccgaagcgct ggcgacgcgc gtggtaaacc ggacgggtgg
     1861 acatgccatg tttaatattc ttcgccgcat aattttgcaa gacttcttgt cgctcggaat
     1921 caatggcctc gcaggacttg ctcgatttga gaagcgactt gggcggcctg caaggcgatg
     1981 ccctcccggt tgaaatggat gttgtcccgg tggaattcgg gatgtccatt catgagcgag
     2041 c
//
LOCUS       DASZMM010000524         5624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4898511, whole genome shotgun sequence.
ACCESSION   DASZMM010000524 DASZMM010000000
VERSION     DASZMM010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4898511"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VG754_12625"
     CDS             <1..720
                     /locus_tag="VG754_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HWF20106.1"
                     /translation="AQIPEARLRGYSPSRFSFNSAQGRCPECEGAGTIKLEMNFLPPA
                     FVRCEVCGGCRFNRETLDILFNGKNIAQVLELSVDEALEFFSAFAKIKRPLQALCDTG
                     LGYLKLGQTSPTLSGGEAQRVKLVTHLLSGLHEPDLIERVPKHNLFILEEPTIGLHIA
                     DVRRLVDVLQRLVDAGHSVIVIEHNLDLIAEADWVIDLGPEGGEAGGTLVAEGTPEMV
                     ARNKRSHTGRFLRSFLPAPNV"
     gene            877..1347
                     /locus_tag="VG754_12630"
     CDS             877..1347
                     /locus_tag="VG754_12630"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWF20107.1"
                     /translation="MRDPYEEILEGEICLRLPPGERHEAICERLHTRVAGSLASLTCA
                     RLLPARSPVQLSRETKVRPDLALVAAASNKLWLVAEVVNSGDHNPDTVLKKTLYEQAH
                     LPRLWMVDPRYNNVEVYHGSEHGMVLKQILAIREILSEPLLPKFQYVIEDLFKA"
     gene            1379..2989
                     /locus_tag="VG754_12635"
     CDS             1379..2989
                     /locus_tag="VG754_12635"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HWF20108.1"
                     /translation="MKIAIGLTVLVMGALFSMHIHAETYDLIIRHGRVVDGTGNPAFF
                     ADVAVTNGRIAAIGVVNGEAKQEVDAKGLIVAPGFIDVHTHADDVADLPLAENFVRMG
                     VTTIVVGNCGGSASDIAKLFHDIEEAKVSVNVASLIGHNTVRSRVMGGSFMRPPTAEE
                     MDKMKAMVEQGMKDGAVGLSTGLIYLPGTFAKTEEIVELAKMASAYDGIYTSHMRDEG
                     TNIFDSLNELFRIAREAHIRAEISHIKLSGNASWGQTSEVLAAIEKARDEGLDITEDQ
                     YAYTASSTGISQLVPSEAREGGRDKFLERLGDPKQKAVIVAGMKDMLAGNKRKDYTYA
                     VIAEYKTDPSLNGLTVPQATKKKRGSDSLDEQIEMILDIESHHGASGVFHGINEDDLQ
                     QFMKAPNTMFASDSGVREFGKGVPHPRGYGNNARVLARYVRELHVLRLEDAIRRMTTL
                     PAETFHLTDRGQLRVGAWADISVINPASVQDNATYTDPHHYATGIPFVFVNGVEVLKD
                     GEHTGAKPGMALRHQQPMKSEITPQPKP"
     gene            complement(2967..3269)
                     /locus_tag="VG754_12640"
     CDS             complement(2967..3269)
                     /locus_tag="VG754_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWF20109.1"
                     /translation="MKSKLLANITPGEILEEDFIKPMKITQYRLAKDIGVPPRRINEI
                     VKGQRAITADTALRLGRYFSMSPEFWLNLQSHHDLEQENERLGGRLDKEVKVLAAA"
     gene            complement(3272..3553)
                     /locus_tag="VG754_12645"
     CDS             complement(3272..3553)
                     /locus_tag="VG754_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF20110.1"
                     /translation="MILSFACGETERVFKAELSRRFPHTIQRIARRKLLALHAATELR
                     DMFVPPGNRLEALKGSRKGQHSIRVNDQWRICFRWSDGNAHDVEIVDYH"
     gene            complement(3645..>5624)
                     /locus_tag="VG754_12650"
     CDS             complement(3645..>5624)
                     /locus_tag="VG754_12650"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HWF20111.1"
                     /translation="VQTGSKVIATEFTLPQGLPTGTYSLFAVANGNPSVPQTFTYSPP
                     PIPSGLTALSGSNAFVKVSWNASSGATAYNLKRATSSTGYYTNLAMLTGLAYTNTGLT
                     NGLTYYYKVAAIGSSGPSSDSAAVNATPAGQPIIPGATSVSLASAYNRTGIVTDGRTF
                     STGFDGGGSAYSANLLGPSVAWNNLIFTFGPVNASDVVSCTGQTITLSAGRFNSLQIL
                     GAGVNGSQQAQTFTVTYTDNSTATFTQSFSDWANPQSYPGEFTVVSKMPYRDQSNGGT
                     QTLNMAVFGYEFTLDQTKTVKSIALPANANLVLMCIMLANDPVQATLANFYNRPGIYT
                     DGTTYTNPLTGGIDGGGYSYSGTLLGNSQMWSNILFAFGPLNATNVISCSNQTIPLPP
                     GNYSQLRMLGTGVQGNQASQSFVVTYSDTSSTTFIQGMSDWFTPQNYSGETKVVPMSY
                     RNNTNGTSSENSTLYLYGYFFNLNAAKTVQSIRLPNDGNAIVLAISVVPDWPPTFKLN
                     PFTLANATAGQAYSGTIATNAGDLNGDTMTFSKVSGPAWLNVAGNGILSGTPLSPNAG
                     SNSFVVQVADPGGLSSTATMNIQVIPAQPIVLSTIAQSGSMTLNWSGGIAPYQVHVAT
                     DLTIQDWQNYGPPTNATSVMVPSTNNAAYFQVSGN"
BASE COUNT         1288 a         1504 c         1577 g         1255 t
ORIGIN      
        1 gcgcagattc ccgaagcgcg gttgcgcgga tattcgccga gccggttttc attcaacagc
       61 gcgcaaggcc gatgccccga atgcgaaggc gcaggcacca tcaagctcga aatgaatttt
      121 ctgccgcccg cgttcgtgcg ctgcgaagtt tgcggcggct gccggttcaa tcgcgagacg
      181 ctcgacatcc ttttcaacgg caaaaacatc gcgcaagtgc tcgaattgtc cgtggacgaa
      241 gcgctggaat ttttctccgc gttcgcaaag atcaaacgac ctttgcaggc actgtgcgac
      301 accggccttg gctacctgaa actcggccag accagtccca cgttgagcgg cggcgaagcc
      361 cagcgcgtca agctggtgac gcatttgttg tccgggttgc acgaaccgga tttgatcgag
      421 cgggtgccga agcacaacct gttcatcctt gaagagccga cgattggcct gcacatcgct
      481 gacgtgcggc ggcttgttga cgtgttgcaa cggcttgtgg atgcgggcca ttcagtcatt
      541 gtcatcgagc acaatcttga ccttatcgcc gaggcggatt gggtgattga tttggggccg
      601 gaaggcggcg aagctggcgg gacgctcgtc gccgaaggta cgccggaaat ggtcgcgcgc
      661 aacaaacgct cgcataccgg aagatttctg cgtagtttcc tccctgcacc aaacgtgtag
      721 cgcagagttg aactctgccg tatcgcggag ttgcactccg catgggatag gaatttcgag
      781 aaagctttgg aattttccaa accgctctct cttatttcgc cctgccgact ggaagtcggc
      841 gatacagcag attggaaatc tgcgctacga gaaagcatgc gcgacccgta cgaagaaatt
      901 ctggaaggtg aaatctgcct gcgtttgccg cccggcgaac gccatgaagc catctgcgaa
      961 cgcctgcaca ctcgcgtcgc cggaagcctt gcttcgctga catgcgcgcg tttgctccct
     1021 gcccgctcgc ccgtgcaatt gtcccgtgaa accaaagtcc gtcccgacct cgcgctcgtg
     1081 gctgccgcga gcaacaaact ttggctcgtt gccgaagtcg tgaattccgg cgaccacaat
     1141 cccgacaccg tcctcaagaa aacactctac gaacaagccc acctgccgcg cctctggatg
     1201 gtcgatccgc gctacaacaa tgtggaagtt tatcatggca gcgaacacgg catggtcttg
     1261 aaacaaatcc tcgccatccg cgaaatcttg agcgaaccgc tgctgccgaa atttcaatat
     1321 gtcatcgagg acttgttcaa ggcatgaatg gaaaggcttt tgtgatatgg ttcaaaaaat
     1381 gaaaatcgca atcgggctca ccgtgttggt gatgggtgct ttgttttcaa tgcacattca
     1441 cgctgaaacc tacgacctga ttatccggca cggtcgcgtg gtggatggaa cgggcaatcc
     1501 ggctttcttc gcggatgtcg ccgtgaccaa tgggcggatc gcggcaatcg gtgtcgtcaa
     1561 cggcgaagca aaacaggaag tggatgcgaa gggactcatt gtcgcgcccg gtttcattga
     1621 cgttcacacc cacgcggatg atgtcgcgga tttgccgctc gcggaaaatt ttgtccgcat
     1681 gggtgtgacg accattgtgg ttggcaattg cggcggttct gcatcggaca tcgccaaatt
     1741 atttcacgac atcgaggaag caaaggtttc cgtaaacgtc gcttcgctca tcgggcacaa
     1801 caccgtgcgg tcgcgtgtca tgggcggctc gttcatgcgg cctccgactg ccgaagagat
     1861 ggacaaaatg aaggcgatgg ttgagcaagg catgaaggac ggcgcggtgg ggctttcgac
     1921 gggattgatt tatctgcccg gcacgtttgc gaagacggag gaaatcgttg aactcgcaaa
     1981 gatggcgtcg gcgtatgatg ggatttatac gagccacatg cgggacgagg ggacgaacat
     2041 ttttgattcg ctcaatgaac tgttccgcat cgcgcgcgag gcgcacatcc gtgccgagat
     2101 ttcgcacatc aaactttccg gcaacgcatc gtggggacag accagcgagg tgcttgcggc
     2161 aattgagaaa gcgcgtgacg agggtctgga catcacggag gaccaatacg cctatacggc
     2221 ttccagcacc ggcatcagtc aacttgtgcc gagcgaagcg cgcgaagggg ggcgtgataa
     2281 gttcttggaa cgcctcggcg atcccaagca aaaggcagtc atcgtcgccg gcatgaagga
     2341 catgctcgca ggaaacaaac gcaaggatta cacctatgcc gtcatcgccg aatacaaaac
     2401 tgatcccagc ctgaacgggc taactgttcc gcaggccaca aagaaaaagc gtggcagcga
     2461 ctcgctcgac gagcaaatcg aaatgatttt ggacatcgaa tcgcatcacg gcgcttcggg
     2521 agttttccac ggcatcaatg aagatgatct gcagcagttc atgaaagcgc ccaacacgat
     2581 gtttgcgtct gacagcggtg tgcgggaatt cggcaaaggc gtgccgcatc cgcgcggtta
     2641 cggcaacaat gcgcgcgtgc tggcccgtta cgtgcgcgaa cttcatgtgc tgcgattgga
     2701 agacgccatt cgccgcatga ccaccctgcc tgcggaaacc tttcatctca ccgatcgcgg
     2761 gcaattacgc gtcggcgctt gggccgacat cagcgttatc aatcccgctt cagtccaaga
     2821 caacgccacc tataccgacc cgcaccatta cgcgaccggc attccttttg tattcgtgaa
     2881 tggcgtggaa gtgcttaaag acggcgagca caccggcgca aaaccgggca tggcgttgcg
     2941 gcatcagcag ccaatgaaga gtgaaatcac gccgcagcca aaaccttgac ctctttatcc
     3001 aggcgccctc ccaagcgctc attctcctgc tccaggtcat gatgcgattg cagattcagc
     3061 caaaactccg gcgacatgct gaaataccgg cccagtctta acgccgtatc cgcagtgatg
     3121 gctcgctggc ctttgactat ttcattgatg cgccgaggag gaacaccgat gtccttcgcc
     3181 aaacggtact gagtgatttt catgggcttt atgaaatcct cttcaagaat ttctcccggc
     3241 gtgatgttgg caagcagttt gcttttcata attagtgata gtccacaatt tcaacgtcgt
     3301 gggcatttcc atcgctccag cgaaaacaaa ttcgccattg gtcattgaca cggatactat
     3361 gctggccctt gcggcttcct tttaaagctt caaggcgatt gccaggtggc acaaacatgt
     3421 ccctcaattc cgttgcggca tgcaacgcca gcagtttgcg tcgcgcgatt cgttgaatcg
     3481 tgtgcggaaa tctccgcgac aattcagctt tgaacaccct ctctgtttcg ccgcatgcaa
     3541 aacttagtat caccaagcga tgttaacgct tcacgttaat agcgtcaaca ttgttattcg
     3601 cggcccaaaa aatggaggga cctgcaaatg tccttcatca agaatcaatt tccagagact
     3661 tgaaagtaag ccgcattatt cgtcgatggc accatcacgc tggtggcatt tgtcggcggg
     3721 ccgtagtttt gccaatcttg gatggtcaaa tccgtggcga catgcacttg atatggcgcg
     3781 atgccaccgg accaattgag tgtcatcgag ccgctttgcg caattgtgct tagaacgatc
     3841 ggctgggcgg gaatcacctg gatgttcatc gttgccgtgc tggaaagtcc gccgggatcg
     3901 gccacctgaa ccacaaatga attgctccca gcgttgggcg aaagcggcgt tccgctcagg
     3961 attccgttgc cggcaacatt caaccacgcc ggaccgctga ctttggaaaa tgtcattgtg
     4021 tccccgttca aatcgccggc gttcgtcgca atcgttcccg aatacgcctg tcccgcggtt
     4081 gcatttgcaa gcgtgaatgg attcagcttg aatgtcggcg gccaatccgg aaccacactg
     4141 atcgcgagga caatcgcgtt gccgtcgttt ggcagacgga tgctttgtac ggttttcgcg
     4201 gcgttgagat tgaaaaaata gccgtacaga taaagggtgc tgttttccga tgaagtgcca
     4261 ttggtattgt tgcgataact catgggcaca actttcgttt cgccggaata attttgcggc
     4321 gtgaaccagt cgctcatgcc ctggataaat gtggtcgagc tggtgtcgga gtacgtgacc
     4381 acgaaagatt gtgaagcctg gttgccctgc acgcccgttc caagcatacg cagttgcgaa
     4441 taattccccg gcggcaacgg aatggtctga ttcgagcaac tgatgacgtt ggtggcattc
     4501 aacggcccga acgcaaataa gatgttgctc cacatttgcg aattgccaag caacgtgccg
     4561 gaatacgaat aaccgccgcc gtcgatgccg ccggtcagtg gattggtgta agtcgtgccg
     4621 tccgtgtaaa taccgggacg gttataaaaa ttcgccagtg ttgcctggac aggatcattg
     4681 gccagcatta tgcacataag aacgagattg gcattggcgg gcagcgcgat gcttttgacc
     4741 gttttggtct ggtccagggt gaactcatag ccaaaaacgg ccatgttcag agtttgcgtg
     4801 ccgccgttgc tttggtccct gtaaggcatc ttggagacga cagtgaattc gccgggatac
     4861 gattgcgggt tggcccagtc gctgaaactt tgagtgaatg tggccgttga attatccgta
     4921 taagttacag tgaatgtttg cgcctgctgg ctgccgttca caccagcgcc gagaatttgc
     4981 agtgaattaa atctgcccgc cgacaaagtg attgtctggc ccgtacaaga aacgacgtcc
     5041 gatgcattca caggcccaaa agtaaaaatc aaattgttcc acgcaaccga cggaccaagc
     5101 agattggcgg aataagccga accgcctccg tcaaatccag tggaaaacgt gcgtccgtcg
     5161 gtgacgattc cggtgcgatt ataagctgaa gcgaggctga ccgaagtcgc ccctggaatg
     5221 atcggctgtc cagccggggt cgcgttgacc gcggcggaat cagaacttgg cccgctgctg
     5281 ccgattgcag cgactttgta ataatacgtg agcccattgg tcaatccggt gtttgtgtat
     5341 gcaagcccgg tgagcatcgc aagattagtg tagtagccag tgctggacgt ggcgcgtttc
     5401 aagttgtatg ccgtcgcgcc cgaactggca ttccagctta cttttacaaa tgcgttgctg
     5461 ccactgagtg cggtcaaacc agaaggaatt ggcggcggcg aataagtgaa tgtctgggga
     5521 acggaaggat tgccattggc gacggcaaac agggaataag tgccggtcgg aagaccttgc
     5581 ggcagtgtaa attccgtggc aatcaccttg cttcccgtct gaac
//
LOCUS       DASZMM010000525         7659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4081434, whole genome shotgun sequence.
ACCESSION   DASZMM010000525 DASZMM010000000
VERSION     DASZMM010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4081434"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VG754_12655"
     CDS             <1..1006
                     /locus_tag="VG754_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF20112.1"
                     /translation="AGGAVVNQMGIAQLNKKSRLLDYQATVNKVMMDVRIAFYDVLRS
                     QAGIAVHQQAVDFLQKQLQYERQRLEVGTGQKLNSMRAEVELSLEQAALIESQNLSRN
                     ASLHLDELLAIASGSNGMPFAVEGQLEIQPLAFNEEQCTVNALEHRPELQVSENNIAV
                     QKKQYVVDRSAILPHVNLFVGYDVVSEPDRALPDEYYKGYTMGVGVSWNIFDGFAAKG
                     RMRETRARMEEAEIAESALKNGVRAEVARAFHDLQRAAETVQSQRENMDLAAQSLSLA
                     QANFQEGLITQLDLLQSQLDLTRAQSVELNARFDYNSALAQLERAMGAEFDVTTKGAK
                     "
     gene            1003..2331
                     /locus_tag="VG754_12660"
     CDS             1003..2331
                     /locus_tag="VG754_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF20113.1"
                     /translation="MKRALAIVALVLAGWVNGGHSAPAETNIPVTLSEGFQKVLAHDP
                     ELLRLRKDVERATGTKLVFHARDLAQFGVQPTVGLEGGSLYGNHSKFALVTAQFSQPL
                     LNAAIAPNWRRGNLEVLATEQNLNAAIAGRLHEFRIYYIRAQRLQQLIALYEEIDRRL
                     QTNVLAEQQRREAGTTGPRPLLQAKVQLLSERAELTGFRREDFEVRSAMAEMMGQSPE
                     RLPLPTEPLAREKVNMDLKADTEAAERQRADLKFLRALIGMAKEDERVVGADYFPYVS
                     AIASSLYIPGKRPVYQATPIIEGQQPLGTDYRGGVNLTWQIIDNGRVTGVRRQIKGLR
                     EEYAIVLGQLQENIPRELERISHSLENADAKLNALEKSTTEADQGLKLIETRISLGEA
                     TQLDFSNAQRDLLSVRHGIVDALFQQAWAKADLDRVTGTYLEFAERVKGP"
     gene            2349..3476
                     /locus_tag="VG754_12665"
     CDS             2349..3476
                     /locus_tag="VG754_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF20114.1"
                     /translation="MSKKVFALVLLAIAAVAGIYIYRSSRPVVEVFLARRGTATYAVY
                     GTVKVVPMVSFSIHARSGGVLKMSEALAYATNLIGMEITNGQYLGEVVSESLDRDFAK
                     ASAEWEAAQARQKLGPAGLPMLKTQESLVERFQKLAAQNNIAPSDVERATNDLATLRD
                     AVHQQQIELDRSTEVFHQAYEDQKERKERCKFISLIDGVINAVNNANGEFIPEGSTPF
                     VLETKATYIEGQVNEEDVGHVAPNMKAAVKLYAYPDRELPATVAQVLPTANNQRYTVN
                     LNLDKPPDNLLPGETGEMNIICGTRDNALLIPSRALLADRVWVVRDGVAAPREVKVGY
                     RNIERAEILDGLHDGEAVVVADQDLLRSGMRVRQVVLNPPD"
     gene            3480..4727
                     /locus_tag="VG754_12670"
     CDS             3480..4727
                     /locus_tag="VG754_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF20115.1"
                     /translation="MSPPLFIALRFIRHRQRSILISLAGITLGVAFFICTQAQTQGFE
                     QFYIQTVLGTSGAVLVQDRFQDRYTGILEQKTNDMVSVSGQKPRKYYDGITDPERIMR
                     VVREFSTVQACAPVLEDNASILTDFRSDVCRVLGIDLEAQLAATDLRAYLIDGDLGTF
                     RTTPAGVLLGSLLALKMHVRVGDTVSLMAPGGETRNYRICGIAQTGDNLIDERRAYIH
                     LGEAQSLLGKPAAVSYIIVKMNDPDRAPQLATRLEQLLQHRARSWQERASGNLQIFRA
                     IRISAAITVSAIIMLAGFGIFNVLTLMIIDKVREIAILRSMGYQRQDISAIFLWQGFL
                     VALAGSVVGCILGWALTYGVSRVPMHIRGFFAADHFMVHWSWLHYLMAVVIAFAAVLL
                     ASYFPSRRAARLAPVAILRGAGQ"
     gene            4724..5419
                     /locus_tag="VG754_12675"
     CDS             4724..5419
                     /locus_tag="VG754_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF20116.1"
                     /translation="MNQPLVCEGLHRSLGENEARVQALRGVSLEIPAGSVHAIVGPSG
                     CGKSTLLYLLGLLDNADAGRLWIGGVDVAGMSEPEMTRKRNEMIGFVFQFHFLMREFT
                     AEENVMIPMRRLGRLAPAQMRERAGMLLETVGLADKRNRLSGQLSGGEQQRVAIARSL
                     ANDPQIVLADEPTGNLDTANSERVFGLLTSLVKREKKALVLATHNPLIAQASDCVHEM
                     KDGLITRAYTNTR"
     gene            5416..6321
                     /locus_tag="VG754_12680"
     CDS             5416..6321
                     /locus_tag="VG754_12680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF20117.1"
                     /translation="MNSAIAPVQKKVVAPQQAAEISFLVPMYNGLELTRACLRSLEET
                     VDLARHEVVLINDASTDGTAEFLATLSAPYRVIHNSQRSSYAASINRGVALARGEMLC
                     LLNNDLVFSPGWLPPMLQAFERFQNVGIVGNVQIDGRTGRYNHMGMLFSEDGVPSHFG
                     EHFPFRPFRGWTEWKAVTSACWLVRKSVFLAGGGYNEAYRNAGYEDTELCLSLLKLGY
                     RHYVANESVIKHFVNSSEGRLQFESQNAQYFGERWHDYIVSQFTAQDRRRIAANYLLR
                     CLGELSRCRVKKLGKALATLLTCGH"
     gene            6309..7328
                     /locus_tag="VG754_12685"
     CDS             6309..7328
                     /locus_tag="VG754_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF20118.1"
                     /translation="MRTLILSRPDRIGDVVISTSCLAPIREKFPEAKIFFVAAERMRP
                     LLENHPLLAGFIPVTADLIKEFQRLDASAIVHLHPNQDCYVAASRAGVPMRIGYPVRF
                     LNRHLTHKIEDRRKEGLQHEAAYNFDLLQALDVALPKQLFANVHLPKVARETLQHKLP
                     WPVASTPFAVLHPSAHSKIARWPAERFASMAESLKESFGFVPVFIGAGANDFSVSGMS
                     SHLNLSGQTDLGELGWLLKHARVLVTNDSGPAHLAAAVGCPVVSIFGRTAPMYGPARW
                     RPLSERAVIVIKPLARKRFEGRDAHWRRCFAAIELEEVKSAVRQALGAETEIKAFAQP
                     CTPEK"
     gene            7527..>7659
                     /locus_tag="VG754_12690"
     CDS             7527..>7659
                     /locus_tag="VG754_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF20119.1"
                     /translation="MKIKTVRSGGFTLVEIMIVVAIIGLLATIAIPNFVRARLKAQQS
                     "
BASE COUNT         1649 a         2025 c         2331 g         1654 t
ORIGIN      
        1 cgctggtggc gcggtggtga accaaatggg catcgcccaa ctcaacaaga agtcgcgcct
       61 gctcgattat caagcgaccg tcaacaaggt gatgatggac gtgcgcatcg ctttttacga
      121 tgtgttgaga agccaggcgg gcatcgcggt gcatcagcag gcggtggatt ttctccaaaa
      181 acaattgcaa tacgaacgtc agcggctcga agtcggcact gggcaaaaat tgaactcgat
      241 gcgcgccgag gtggagcttt cgctcgaaca ggcggcattg attgaatcac agaatctctc
      301 gcgaaatgcg tcgctgcatt tggacgaatt gctggcgatt gcatccggtt caaacgggat
      361 gccgttcgcg gtcgagggac aacttgaaat ccagccgctg gcgttcaacg aggagcaatg
      421 cacggtcaac gcgctggagc accgcccgga attgcaggtg agcgagaaca atatcgcggt
      481 gcaaaagaaa caatatgtgg tggaccgcag cgcaatcctg ccgcatgtga atctgttcgt
      541 cggatacgac gttgtgagcg aaccagaccg ggcgttgccc gacgaatatt acaagggcta
      601 cacgatgggc gtgggggtga gctggaacat ttttgacggg ttcgccgcga aaggccggat
      661 gcgcgagacg cgcgcgcgga tggaggaggc cgaaatcgcg gaatcggcgc tgaaaaacgg
      721 tgtgcgggcg gaggtggcgc gcgcatttca tgatctgcaa cgagcggcgg aaacggtgca
      781 atcacagcgc gaaaacatgg accttgcggc gcaatcactg tcgctggcgc aggcgaattt
      841 ccaggaaggg ctcatcacgc aactcgattt gctgcaaagc cagcttgacc tcacgcgcgc
      901 gcaatcggtg gaattgaatg cgcgcttcga ttacaacagc gcgctcgcac aattggagcg
      961 cgcgatgggc gccgagtttg acgtcaccac caagggagca aaatgaaacg cgcacttgcc
     1021 attgtcgcgc tggttcttgc gggttgggtg aatggcggcc attccgcgcc agcggagacg
     1081 aacatccccg tcacactttc ggaaggtttt caaaaagtgc tcgcgcacga cccggagttg
     1141 ctgcgcttgc ggaaggatgt cgagcgcgcg accggaacga aactagtttt tcacgcgcgc
     1201 gacctggcgc agttcggtgt ccagcccacg gtcggattgg aaggaggcag tttatacggg
     1261 aaccattcca agtttgcgct ggtcacggcg caattttcgc agccactgct aaatgcggcc
     1321 atcgcgccga actggcggcg aggcaacctg gaagttttgg cgacggagca gaacttgaac
     1381 gcggccatcg ccggacggtt gcacgaattc cgcatttatt atattcgcgc gcaaaggttg
     1441 caacaactca tcgcgctgta cgaggagatt gaccggcgtt tgcagacgaa cgtcctggcc
     1501 gagcagcaac gccgcgaggc cggcaccacc ggcccgcgtc cattgctaca ggcgaaggtg
     1561 caacttttgt ccgaacgggc tgaactcacc ggattccgcc gcgaagattt cgaggtgcgt
     1621 tcggccatgg ccgaaatgat gggccaatcg ccggagcgtt tgccgctccc gaccgaaccg
     1681 ttggcgcggg agaaagtcaa catggatttg aaagccgata cagaagcggc ggagcggcag
     1741 cgtgccgatt tgaaattttt gcgcgcgttg attgggatgg cgaaggagga tgaacgcgtc
     1801 gtgggagcag attattttcc atacgtttcg gcgatcgctt catctcttta cattcccggc
     1861 aagcggcccg tttaccaggc gaccccaatc atcgaagggc agcaaccgct cggaacggat
     1921 tatcgcggtg gggtgaattt gacctggcag atcattgaca atgggcgggt caccggcgtg
     1981 cgacgacaaa tcaaagggtt gcgcgaggaa tacgcaatcg tgctgggcca attacaggaa
     2041 aatattccgc gcgaattgga acgcatatcg cactcgctcg aaaacgccga cgcaaaactc
     2101 aatgcgctcg aaaaatccac gaccgaagcg gaccagggtt tgaagctcat cgagacgcgc
     2161 atttcgctcg gtgaagcgac gcaactggat ttttccaacg cgcaacgcga tttgctgtca
     2221 gtacgtcatg gcattgtgga tgcattattc cagcaggctt gggcgaaggc ggatttagat
     2281 cgcgtgaccg gcacatattt ggaatttgca gagcgggtga aggggcctta aaatccccaa
     2341 ttgtactgat gagtaaaaaa gtttttgcgc tcgtgttgct ggcaatcgcg gcagtggcgg
     2401 gcatttacat ctaccggtcg tcacggccgg tagtggaagt ttttttggcg cggcgcggca
     2461 cggcgactta cgccgtgtat ggcacggtga aggtggtgcc gatggtttcc ttttccatcc
     2521 atgcccggag cggcggtgtg ctcaagatga gcgaagcgct tgcctatgcc accaacttga
     2581 taggaatgga aatcacgaac ggtcaatatc tcggcgaagt cgtgagtgaa agtttggacc
     2641 gcgattttgc aaaggccagc gccgagtggg aagcggcgca agctcgccaa aaacttggtc
     2701 ctgctggttt gccgatgttg aagacgcagg agagcctcgt ggaacggttc cagaaacttg
     2761 ccgcgcaaaa taacattgcc cccagcgacg tcgagcgcgc aacgaacgac cttgcaacct
     2821 tgcgtgacgc tgtgcatcaa cagcagattg aactggaccg ttccacggaa gtgttccatc
     2881 aggcatacga agatcagaag gagcgcaagg aacgctgcaa attcatttct ctcattgacg
     2941 gggtaatcaa cgccgtcaac aacgccaacg gcgagttcat tcccgagggc agcacgccgt
     3001 tcgtgctgga gacgaaagcg acttatatcg aaggccaggt gaatgaagaa gatgtcggcc
     3061 acgtcgcccc gaacatgaaa gcggcggtga aactttatgc gtatcccgac cgcgagttgc
     3121 cggcgacagt tgcgcaagtc ctgccgacgg cgaacaacca gcgttacacg gtcaatttga
     3181 atctggacaa gccgccggac aatttgctgc ctggcgaaac aggcgaaatg aacatcatct
     3241 gcggcacgcg cgataatgcg ttgctcattc catcgcgcgc cttgcttgcc gaccgggttt
     3301 gggtggtgcg ggacggcgtg gccgcgccgc gcgaggtaaa ggtcggctat cgcaacattg
     3361 aacgcgcgga aatccttgat ggcttgcacg acggcgaagc ggtggtcgtt gccgaccagg
     3421 atttgttgcg cagcggaatg cgcgtgcggc aggtggtgct caacccgcct gattgagcca
     3481 tgtcgccgcc gcttttcatc gcgcttcgat tcatccggca tcgccagcgc tcgattttga
     3541 tcagcctcgc gggaattacg ttgggcgtgg cgtttttcat ctgcactcag gcgcaaacgc
     3601 aaggcttcga gcaattttac atccagacgg ttctcggcac gagcggcgct gtgctggtgc
     3661 aggaccgttt tcaagaccgt tacaccggaa ttctcgaaca gaaaacaaac gacatggtga
     3721 gcgtcagcgg ccagaaaccg cgcaagtatt acgatggcat caccgacccg gaacgcatca
     3781 tgcgggtcgt gcgcgaattc tccaccgtgc aggcttgtgc gccggtgctg gaggacaacg
     3841 cgtccatcct gaccgatttt cgcagcgacg tttgccgcgt gctgggcatt gacctggaag
     3901 cgcaactcgc cgcaaccgat ttacgcgcat acttgattga cggcgatttg gggacgttcc
     3961 gcaccacacc ggcgggcgtt ttgcttggct ctctgctcgc gctcaagatg cacgtgaggg
     4021 tgggcgatac agtttccctg atggcaccgg gtggcgaaac gcgcaattac cgcatctgcg
     4081 gcatcgcgca gaccggcgac aatttgattg acgaacggcg cgcttatatt catttgggcg
     4141 aggcgcaaag tttgctcggc aaacctgcgg cagtttccta catcatcgtc aaaatgaacg
     4201 atcccgaccg cgcgccgcaa cttgcgacgc gtctggaaca actgctgcaa catcgcgcgc
     4261 gaagctggca ggaacgggcg agtggaaact tgcagatttt tcgcgccatc agaatttcgg
     4321 cggcgatcac ggtctcggcc atcatcatgc tggcgggctt tgggattttc aatgtgctga
     4381 cgttgatgat tattgacaag gtgcgggaga tcgcgatttt gcgctcgatg ggttatcaac
     4441 ggcaggacat cagcgcaatt tttctttggc agggattttt ggtggcgctg gcgggttctg
     4501 tcgtcggatg catcctgggc tgggctttga cttacggtgt gtcgcgtgtg ccgatgcata
     4561 tccgcggttt ttttgccgcg gaccatttca tggtgcattg gagctggctt cattatctga
     4621 tggcggttgt gatcgcattt gcggcggtgt tgctcgccag ttattttcct tcacgacgcg
     4681 cggcgcggct tgcgccggtg gcgatcttgc ggggggcggg ccaatgaacc agccgctcgt
     4741 gtgcgaagga ttgcaccgca gcctcgggga aaacgaagca cgggttcagg cattgcgcgg
     4801 cgtgtcgttg gaaattcccg ccggatcagt ccatgccatc gtgggaccat ccggttgcgg
     4861 caaaagcacg ctgctttatc tgctgggcct gctggacaac gcggacgcgg ggcggctttg
     4921 gatcggcggg gtggacgtgg ccggaatgtc cgaacctgaa atgacccgca aacggaatga
     4981 gatgatcggc ttcgtgttcc agtttcattt tttgatgcgc gaatttaccg ccgaggaaaa
     5041 tgtgatgatc ccgatgcggc gtttgggacg gctcgcgccc gcgcagatgc gcgaacgcgc
     5101 cgggatgttg ctggaaactg tcggacttgc ggacaaacgc aaccggctca gcggccaact
     5161 ttccggcggc gaacaacaac gcgtggcgat tgcgcgctca ttggccaacg acccgcaaat
     5221 cgtgcttgcg gacgaaccga ccggcaattt ggacactgcc aattcggagc gcgtgttcgg
     5281 cctcttgacg tcgctggtga aacgcgagaa aaaggcgctt gtgttggcga cgcacaatcc
     5341 gctcatcgcg caggcgagcg attgcgtcca tgaaatgaaa gacggcttga tcacccgcgc
     5401 ttacacgaac acccgatgaa ttccgcaatc gcgccagttc agaaaaaggt ggtcgcgccc
     5461 cagcaagctg cggagatttc gtttctcgtc ccgatgtata acggcctgga gctgacgcgc
     5521 gcctgtctgc gcagtctgga ggaaacagtt gatcttgcac ggcacgaagt cgttttgatc
     5581 aatgacgcca gcacggacgg gacggcggaa tttttggcga cgctctccgc gccgtatcgg
     5641 gtcattcaca actcgcaacg gtccagttac gcggccagca tcaatcgtgg cgttgcgttg
     5701 gcgcgcggcg aaatgctttg tttgctcaac aacgatcttg ttttcagccc gggttggctg
     5761 ccgccgatgt tgcaggcgtt tgagcgattt caaaatgtcg gcatcgtcgg caacgtgcag
     5821 atcgacgggc gcacgggcag gtataaccac atgggcatgc tgttctccga agacggcgtg
     5881 ccttcccatt tcggcgaaca ttttcctttt cgcccgtttc gcggctggac ggaatggaag
     5941 gccgtgactt ccgcctgctg gctcgtgcga aaatcggttt ttctcgctgg aggcggctat
     6001 aatgaagctt atcgaaatgc cgggtacgaa gacaccgaac tttgtctgtc gttgttgaaa
     6061 ttgggttacc gccattacgt tgcaaacgag agcgtcatca aacatttcgt gaattcttcg
     6121 gaagggcggc tgcaatttga atcgcagaat gcgcaatatt ttggcgagcg ttggcacgat
     6181 tacatcgtct cgcaattcac ggcgcaagac aggcggcgga ttgcggcaaa ttatctcttg
     6241 cgctgccttg gcgaactttc ccgttgccgc gtcaagaaac tcggcaaggc gcttgccact
     6301 ttgctcacat gcggacactg attctttctc gtccggaccg catcggcgat gtggtgattt
     6361 ccacttcatg cctcgcgccg atccgcgaaa aatttccgga ggcaaaaata ttctttgtgg
     6421 ctgccgaacg gatgcgtccg ttgctcgaaa atcatccttt gctcgcgggc tttattccag
     6481 tcaccgccga tttgataaaa gaatttcagc gactcgacgc ttcagccatc gttcacctgc
     6541 atccgaacca ggattgttac gttgccgcgt cgcgcgcggg agtcccgatg cgcataggct
     6601 acccagtgcg atttctgaac cgtcatttga ctcataagat tgaagaccgc cgcaaagaag
     6661 gcctgcaaca cgaagccgcg tacaattttg atctgctgca ggcgcttgat gtggcattgc
     6721 cgaagcagct tttcgcgaat gttcacctcc ccaaagtcgc tcgtgagacg ttgcaacaca
     6781 aactcccctg gccggttgct tcaactccgt ttgcggtgct gcatccttcg gcgcattcga
     6841 aaattgcgcg ctggcccgct gagcgttttg cgagcatggc cgaatcgctg aaagaaagtt
     6901 ttgggttcgt tcctgtcttt atcggcgcgg gtgcaaatga tttcagcgtt tccgggatgt
     6961 catcgcattt gaatttgtcg ggtcaaaccg atttgggcga gttgggctgg ctgttgaaac
     7021 acgcgcgcgt gcttgtcacc aacgacagcg gacccgctca tctcgctgcg gcggtcggtt
     7081 gtccggttgt ttcgattttt gggcgcaccg cgccgatgta tgggccggca cgatggcgtc
     7141 ctttatcaga acgagcggtc atcgtcatca agccgctggc aagaaaacgg ttcgaaggac
     7201 gcgacgccca ttggcggcgt tgttttgcgg caatcgaatt ggaggaagtg aagtcagcag
     7261 tgcggcaggc gcttggcgcg gaaaccgaaa ttaaagcgtt cgcgcaaccc tgcactccgg
     7321 agaagtaatc gccgtcagac gctggaaaaa gttgctccgt acttttacga agcaccgtcc
     7381 gtatttgtgg cgaattgcaa ggagaagatt gtttgttacg atgagttcca caacaagttg
     7441 ggccgaaacg ggcgtttcac gcaacgactt gttgtaggga atcaaatttg ggtttggtga
     7501 cactgttacg ggaaagcgaa catcctatga agatcaagac tgtccggagc ggagggttta
     7561 cattggtgga aatcatgatt gtggtggcca tcatcggatt gctggcaacc atcgccattc
     7621 caaattttgt gcgcgcgcgt ctgaaggcac agcaaagcg
//
LOCUS       DASZMM010000526         8346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4362026, whole genome shotgun sequence.
ACCESSION   DASZMM010000526 DASZMM010000000
VERSION     DASZMM010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4362026"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VG754_12695"
     CDS             complement(<1..201)
                     /locus_tag="VG754_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20120.1"
                     /translation="MRDTMNRRALLLACLFVAGNIAHLHSEPAALTEVPFAPITNPPP
                     IQMLALGFTVRESPLNLNNINCF"
     gene            547..1566
                     /locus_tag="VG754_12700"
     CDS             547..1566
                     /locus_tag="VG754_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20121.1"
                     /translation="MKHYFLLLLLCGFGMLNARANDTLVFEGTRGPGFGKQIVLLAGD
                     EEYRSEEGLPQLAKVLGGRHGFKCTVLFSINPATGAIDPNFHGNEPGMEALDHADAII
                     MLLRFREWPDAQMKHFVDAYLAGKPIIALRTSTHAFSYDGNSASPYAKFSWNSKTWRG
                     GFGKQVLGETWVSHHGEHKKEATRGIIEASSKDDPILRGVTDIFCTSDVYTVNPPSDV
                     KVLVRGQVLTGMNHDDPPVSGPKNEPMQPIAWTRLYKNEAGATNKILCTTMGAATDLL
                     NEDLRRLIVNSVYWSVGLDVPEHADARLIGDFEPRMYGFDGFAKGVKVDDLKSSKLQH
                     PSAKE"
     gene            complement(1616..4408)
                     /locus_tag="VG754_12705"
     CDS             complement(1616..4408)
                     /locus_tag="VG754_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20122.1"
                     /translation="MEVRFFAPGNLVSNLDFVESIFGNAGDPFLPENDAGLDAEHWTG
                     HTGCIILAPHLTRETKKSLGLPHWDAATERQRRDGMCWRDESEFYNNGSAFKITARDE
                     RGVIVTVIADNYYGYCKKEVKTQISYSANLFGSCEEEHAGGALVYARYDLGEEFDADK
                     HVRYRGHSFEEVLELSGAAMELHPEGFAIDKRFPEIIYVPRGVRFDLQTQTVTWPVAE
                     GERSIKLLADKIYVRPSGYKVQMEKPGNRAWRLIGTVSEGTLCHKPCTVSGGGKSEIS
                     KPICDAIIQGPVFVADFKKDFNQVARLLRRDYSNRFRDAEKNGKDQRSILSHERSLGS
                     VIKLFTPSEREYSDEFNAWLASIPQYLLELVFVVKRFYKPSWGDKWREHFSVDFINGT
                     PGNELKCDNRKLVSNFLRVGYEADGAWRVFGLRKDFHPANKLQMEDDITASVVVPADA
                     LQHLNSDYTNPSVKFVKNCEYRLFQRPDEAVHRGYDKQTEIDLSQPGNFLSNFQPLNV
                     TDARELVEDAVCFSKYSEPMQQLILDVLKKPGPDYFVSSAHPRIVDGKPSKNPRYLQK
                     RPDLVNPRDAYLADMSTRLQRRISLDAPVLTPVNAVMPGRRNNPPDPVSHIRSLAVFN
                     PIHYFELPELFMEFISSMTGKSPSTTGAGSEGALTKGPFNALPPIIDLNAAFVSYALT
                     GYDAFVSAAGHVGPNVRVDHDVSLLIPEVWCRMDVAERKPDFLISKGYFEKCADMEMD
                     GKKVLASRLGYRMTASFVRTFFGRVFNYPYAVFTEEMLRPELQDLAAFADGVDNIVGT
                     HKRVAESYFNDGSVEMACPPLKALLHIMAHGHHENRDVNDPAFRSLFTRESVLASDWY
                     AERLAAKQQQDIRLWHSHATYLQNFLKKQNYAEEAQRLKIEDRLERAWDTYHTVKAPA
                     YLTKLKGTIGLQPLEK"
     gene            complement(4415..4990)
                     /locus_tag="VG754_12710"
     CDS             complement(4415..4990)
                     /locus_tag="VG754_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20123.1"
                     /translation="MIEGINLRLAMAGCPTFGTASLGSMAGPMFSRQKETMRLLADYL
                     CPADWRIDHFLKEYLYETGLPIKWPARTFVLDSPGLARELSLPPDRDEFISDITSSYR
                     LRQGVLHNPAKDRRTTQGVFHVTEGGLPIPDDKLAVPKDVFARLLQSALEPPRELLRL
                     PFTSSQKEQAECFVSLLMRPIVCPAVPGFTG"
     gene            complement(5135..6028)
                     /locus_tag="VG754_12715"
     CDS             complement(5135..6028)
                     /locus_tag="VG754_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWF20124.1"
                     /translation="MIYELTHTTTYDYVNSVSLSHHILRLHPRDLHRQKCLRNKLQIQ
                     PAAAVTQSHLDYFGNEVTFVTVEGPHKHLIITSRSRIEAHASATPKPSETPAWETIGE
                     QCLAAEPNGSVEAREFLFPSPRIKFQSEFADYALPSFTAGRPVLDAVLDLTRRIHEDF
                     KFDPKATTVATPLEQVVKTRRGVCQDFAHFEIACLRMMGVPARYVSGYLETDPPPGKP
                     RLTGADASHAWISFYCPDTGWIDVDPTNNLLPSTRHITVAWGRDYSDVSPIRGVILGS
                     GEHTLKVAVDVIPADEPQLPL"
     gene            complement(6030..>8346)
                     /locus_tag="VG754_12720"
     CDS             complement(6030..>8346)
                     /locus_tag="VG754_12720"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWF20125.1"
                     /translation="EHGVTYNVYGDPHGMDRPWELDMVPLLVPPSEWAKIEAGLVQRA
                     KLFNLILTDLYGPKKLLKDGFLPPALVYANPHYLRPCHGLSVPRQIYLHLHATDLTRS
                     PDGQWWVISDRTQAPSGAGYTLENRIVLSRVLPDEFRDCNVQRLASFFRIHRDTLRSL
                     APHNRDNPSVVLLTPGPYNETYFEHDYLARYLGFTLIEGGDLTVRDSKVFIKTLEGLR
                     QVDVILRRVDDSFCDPLELRGDSFLGVAGLVEAARAGNVAIANALGSGVIESPAFMAF
                     LPGLCRQLLGEELKLPSLATWWCGQEKERQYVIDHLDEFVVKPAFGSLAREPYFGAKL
                     AAKERDELITAIRERPDEFVAQEHATLSTAPVWFNGKLEPRPLVMRAYSTAMGDSYAV
                     MPGGLSRVSTAAEEPIVSMQSGGGSKDTWVISDGPVNMVSLLTPSGQLVRSGRSAAEL
                     PSRVADNLYWLGRYTERLEGTLRLLRCIVGRMVGEAGAEGLPELAGLTQMLMRLDLMP
                     ARFKERIPQKELEHEILLLIYKPDRAGSAKQILNRVRGLASVVRDRFSSDTWSILNKL
                     NIDARSRPHRIPLADALNVLNTAIVDLAAFSGMEMENMTRGLGWRFLDFGRRLERSVQ
                     ISQFFRASIGSQANGSLMLEPVLEIFDSLITYRRRYFTSVQVSSVLELLLLDEGNPRS
                     LAFQLKALREHAAHLPREANTLLESPEQKRIAGLSALLRNADATKLAHPASSNRPENL
                     DSLLADFQVELGMISTQLTHHYFSHTVASVS"
BASE COUNT         1835 a         2227 c         2537 g         1747 t
ORIGIN      
        1 gaagcagttg atgttattga gattgagcgg cgattcgcgc acggtgaatc ccagcgcgag
       61 catctggatg ggcggcggat tggtgatggg agcgaaaggg acttccgtca aggcggctgg
      121 ttcggaatgc aaatgtgcaa tgttcccagc gacgaaaaga caggcgagca acaaagcgcg
      181 gcgattcata gtatcgcgca agggtagggg ttgttttgcc ggttggcgag aaggaaggtt
      241 acgacgacga ttcagactaa tgacaaaaat cattgactga gtggaaattt gaaaacctcg
      301 aataggtttt gtctcaaagc ccggggttgg ctcgattccg agagagccta ccctgggtaa
      361 aagatggtaa aatattccaa cctcgaagag gttgtgacaa tcgcaccttt ggcacaacct
      421 cttcgaggtt gataaatttg ttttgaacgt gttcccaagg tagcgcgttc cgcgcaacct
      481 tgggcttcgt tccgcaacct cttcgaggtt gaaccgaagt ccggccttcg ttaccctgtt
      541 gcatccatga aacattattt cctcctgttg ctgctctgcg gctttggaat gctcaacgcg
      601 cgggcgaatg acacgctagt ttttgaaggc acgcgtggcc caggctttgg caaacaaatc
      661 gtgctgcttg cgggtgacga ggaataccgt tccgaggagg gcctgccgca actcgcaaaa
      721 gtcctagggg ggcgtcacgg gttcaaatgc acggtccttt tttccatcaa tcccgcgact
      781 ggcgcgattg atccgaattt tcacggcaac gaaccgggca tggaggcgct cgaccatgcg
      841 gatgccatca tcatgctgct gcgttttcgc gaatggcccg atgcgcagat gaaacatttc
      901 gtggacgcgt acctcgcggg aaagccgatc atcgcgttgc gcacgagcac gcacgcgttc
      961 agttatgacg gcaactcggc cagcccgtat gcgaaattca gttggaacag caaaacgtgg
     1021 cgcggcggtt ttggaaaaca agtgctcggc gaaacgtggg tttcgcatca cggcgaacac
     1081 aaaaaggaag ccacgcgcgg catcattgaa gcttcttcaa aggacgatcc catcttgcgc
     1141 ggcgtgacgg atattttttg cacgtctgac gtttacaccg tcaatccgcc atcggatgtc
     1201 aaagtgctgg tgcgcggcca ggtgctcacc gggatgaatc acgatgaccc gccggtgagc
     1261 gggccgaaaa atgaaccgat gcagccgatc gcctggacgc ggctttacaa gaatgaagcg
     1321 ggcgcaacga acaaaattct ttgcacgacg atgggcgcgg cgacggattt gttgaacgaa
     1381 gatttgcggc gattgatcgt gaacagcgtt tattggtcgg tcggattgga cgtgccggaa
     1441 cacgcggacg cgcgattgat tggcgatttc gagccgcgca tgtatggctt cgatggtttt
     1501 gcgaaaggtg tgaaggtgga cgatctaaaa agctccaagc tccaacatcc aagcgccaaa
     1561 gaataatcaa gcaccaagct tcaatccacg gaggttggat gttggtgctt ggcgcttact
     1621 tttccagcgg ctgcaatcca atcgtgcctt tcaactttgt caggtaagcg ggcgctttga
     1681 ccgtgtgata agtgtcccac gcgcgttcga ggcggtcttc aatcttcaag cgctgcgctt
     1741 cctccgcata attttgcttc ttgagaaagt tttgaagata cgtcgcgtgg gagtgccaga
     1801 ggcgaatgtc ctgctgctgc ttggcggcga gacgttccgc ataccaatcg ctcgccagca
     1861 cgctctcgcg tgtgaacaat gaacgaaacg cggggtcatt cacgtcgcgg ttttcatgat
     1921 ggccgtgcgc cataatgtgc aacaacgcct tgagcggcgg gcaggccatt tcgacgctgc
     1981 catcgttgaa ataactttcc gcgacgcgct tgtgcgtgcc cacgatgttg tccacgccgt
     2041 cggcgaacgc tgcgaggtcc tgcaattcgg ggcgcaacat ttcctcggtg aaaaccgcgt
     2101 agggatagtt gaagacgcgg ccgaaaaatg tccgcacaaa actcgccgtc atgcgataac
     2161 cgagccggct cgccaaaact tttttgccgt ccatttccat gtccgcgcat ttttcgaaat
     2221 aacctttcga aatgagaaag tccggttttc gttcggcgac atccatgcgg caccagactt
     2281 ccgggatcaa caggctgacg tcgtggtcca cgcgaacgtt cggcccgaca tggcccgctg
     2341 cggaaacgaa ggcgtcgtag ccggtgagcg cataggaaac gaacgcggcg ttcaggtcga
     2401 tgatcggcgg cagggcgttg aacgggcctt ttgtcaatgc gccttcggag cccgcgccgg
     2461 ttgtcgaagg cgatttgccg gtcatgctgg aaatgaattc catgaacaat tccggcagct
     2521 cgaaataatg gatggggttg aacaccgcga gcgagcggat gtgcgaaacc gggtcgggcg
     2581 gattgttgcg acgaccgggc atgacggcgt tgacaggcgt cagcaccggc gcgtccagtg
     2641 agatgcggcg ttgcaggcgc gtggacatgt ccgcgagata cgcgtcgcgc gggttcacga
     2701 ggtcgggacg cttctgaagg taacgaggat ttttggacgg cttgccatcg acgattctcg
     2761 gatgcgcgga ggaaacaaaa taatcggggc ctggtttttt caacacgtcc aaaatcaact
     2821 gttgcatcgg ctcggaatat tttgaaaagc agacggcgtc ctccacaagt tcgcgcgcgt
     2881 cagtcacgtt aagcggctga aaattggaaa ggaaattacc cggctgcgaa aggtctattt
     2941 cggtctgctt gtcgtagccg cgatgcaccg cttcatcggg acgctggaac aaccggtatt
     3001 cgcaattctt gacgaacttg accgaaggat tcgtgtaatc ggaattgaga tgttgcagcg
     3061 catcggcggg aacaacaacc gaggccgtga tgtcgtcctc catctgcagt ttgtttgccg
     3121 ggtgaaaatc cttgcgcaaa ccgaacaccc gccacgcgcc atcggcttcg taaccgacgc
     3181 gcaaaaaatt ggagaccagc ttgcgattgt cgcacttcaa ctcgttcccc ggcgtgccgt
     3241 tgatgaaatc cacgctgaaa tgttcgcgcc atttgtcgcc ccaggacggc ttgtaaaagc
     3301 gtttcacgac gaaaaccagt tccagtaaat attgagggat ggacgcgagc catgcgttga
     3361 attcatccga atattcgcgt tccgacggcg tgaacagctt gatgaccgag ccaagcgaac
     3421 gttcatggct gagaatcgag cgctggtcct tgccgttttt ctcggcatcg cggaaacgat
     3481 ttgaataatc gcggcgcaac aaccgcgcga cctggttgaa atcctttttg aaatcggcga
     3541 cgaacaccgg cccttgaatg atggcgtcgc agatcggttt tgagatttcc gatttgccgc
     3601 cgcccgatac tgtgcagggt ttgtggcaaa gcgtgccttc tgagacggtg ccgatgagcc
     3661 gccaagcgcg attgccaggc ttttccatct gcaccttgta accggaaggc cggacgtaga
     3721 ttttgtccgc gagcaacttg atgctgcgct cgccttcggc gacaggccag gtgacggttt
     3781 gagtttgcag atcgaagcgc acgccgcgcg ggacgtaaat gatttcggga aaacgtttgt
     3841 cgatcgcaaa gccttccgga tgcaattcca tcgcagcacc ggacagttcc agcacttctt
     3901 caaaagaatg gccgcgataa cggacatgtt tgtcggcatc gaattcctcg cccaaatcgt
     3961 aacgcgcata aaccagcgcg ccgcccgcgt gttcctcctc gcacgagccg aaaagatttg
     4021 ccgagtaact gatctgcgtt ttcacctctt ttttgcagta accgtaatag ttgtcggcga
     4081 tgacggtcac gatgacgccg cgttcgtcgc gcgcggtgat cttgaaggcg gaaccgttgt
     4141 tgtagaattc ggattcgtcg cgccagcaca tgccgtcgcg ccgctggcgt tcggtggcgg
     4201 cgtcccaatg cggcaggccg agcgatttct tggtttccct ggtcaaatgc ggcgcgagaa
     4261 tgatgcagcc ggtgtggcct gtccaatgct cggcatcaag cccggcatca ttttccggga
     4321 gaaacggatc gccggcattg ccgaaaatgc tttcgacgaa atccaggttg ctgacgagat
     4381 tgccgggggc gaagaaacgg acttccattt ttttttagcc ggtgaaaccg ggcacggcag
     4441 gacagacaat cggccgcatc aagagcgaca caaaacattc cgcctgctct ttttgcgaag
     4501 aagtgaaggg cagacgcaac aattcccgcg gcggttcaag ggcgctttgc agcaatcgcg
     4561 caaacacatc cttcggcacc gcgagcttgt cgtcgggtat cggcagtccg ccttcagtga
     4621 catgaaaaac gccctgcgtg gtgcggcggt ccttggcggg attgtgcagc acgccttgtc
     4681 gcaaacgata cgaactcgtg atgtcggaaa tgaattcatc tcggtcaggc ggcagcgaga
     4741 gttcgcgcgc aaggccggga ctgtcgagga caaacgtgcg cgcgggccat ttgatgggca
     4801 ggccggtctc gtaaagatat tccttgagaa aatggtcgat gcgccaatcc gccgggcaga
     4861 gataatccgc aagcaggcgc atggtttctt tttgacgtga aaacatcggg ccggccatgc
     4921 tgcccaggct ggcggtgccg aacgtgggac agccggccat cgcgaggcga agattgattc
     4981 cttcaatcat ggcggcgtct gggcggccgt tggctgagtg agattcaggt ggcataacaa
     5041 acaagggaaa aaggacgaat gggacaaata tgacctaatc ttagcggcct tcgcgttctt
     5101 cgcgagaaac aattcttttc cgtcagggcg cgtttcataa tggcagttga ggttcgtccg
     5161 cgggaatcac atccacggca actttcagtg tgtgttcgcc gctgccaagg atgacgccgc
     5221 gaatcgggct gacatcgctg taatcgcggc cccatgcaac cgtgatatgc cgtgtcgagg
     5281 gcaacaagtt gttggtcggg tccacgtcaa tccagccggt gtccgggcag taaaacgaaa
     5341 tccatgcgtg cgacgcgtca gcgccggtga ggcgcggctt gccgggcggc gggtccgttt
     5401 cgagataacc gctcacgtaa cgcgcgggga cgcccatcat gcgaaggcag gcgatttcga
     5461 aatgcgcgaa atcctggcag acgccgcgac gtgttttcac gacttgttcc aacggcgtgg
     5521 cgaccgtcgt ggctttggga tcgaatttaa aatcttcatg gatgcggcgg gtcaaatcga
     5581 gcaccgcatc caaaaccggg cgaccggcgg taaaagaggg aagcgcatag tccgcaaatt
     5641 cggattgaaa tttgattcgc ggcgagggga aaagaaattc gcgcgcttcc accgagccgt
     5701 tcggttccgc ggcaaggcac tgttcgccga ttgtttccca ggcgggagtt tccgaaggtt
     5761 ttggcgtcgc agaagcgtgc gcttcgatgc ggctgcgcga agtgatgatc aggtgtttgt
     5821 gcgggccttc gacagtgacg aaagtgactt cgttgccgaa ataatcgagg tgcgattgcg
     5881 tcacggcggc ggcaggctgg atctgcaatt tgttgcgcag gcatttttgc cggtgcagat
     5941 cgcgcggatg cagacgcagg atgtgatgcg acagcgaaac cgaattcaca taatcgtaag
     6001 tcgtggtatg ggtcagttcg taaatcatat caactcacgc tggcaacggt gtggctgaaa
     6061 taatgatggg tcagttgcgt ggaaatcatg cccagctcga cctggaaatc ggcaaggagc
     6121 gaatcgagat tctccggccg gttggacgaa gcgggatgcg caagcttcgt cgcgtcggca
     6181 ttgcgcaaca gggcggacaa tccggcgatg cgtttttgct cggggctttc aagcagcgtg
     6241 ttcgcctcgc gcggcagatg cgcggcatgt tcgcgcaagg ccttcaattg aaacgcaagc
     6301 gagcgcgggt tgccttcatc gagcagcagc aattcaagca cggacgaaac ttgaacggac
     6361 gtgaaatatc tgcgccgata agtgatcaag ctgtcaaaaa tttcaaggac cggttcgagc
     6421 atcagggagc cgttggcctg gctgccgatg ctggcgcgga aaaattggga aatctgcacg
     6481 gagcgttcga gacgccgccc gaaatccaga aaacgccagc cgagtccgcg cgtcatgttt
     6541 tccatctcca ttccgctgaa cgcggccaga tcgacgatgg cggtgttcaa cacattgaga
     6601 gcgtctgcca gcgggatgcg gtgcgggcgc gagcgcgcgt caatgttgag cttgttgagg
     6661 atggaccatg tgtccgagga aaaacggtcg cgcacaactg aagcgaggcc gcgcacgcgg
     6721 ttcaggattt gtttggcgct tccggcgcgg tccggtttgt agatgagcag gagaatctcg
     6781 tgctccaatt ccttttgcgg aatgcgttcc ttgaagcgcg cgggcatgag gtcgagccgc
     6841 atcagcattt gcgtgagtcc cgccagttcg ggcaggcctt ccgcgccggc ttcgccgacc
     6901 atccgcccga cgatgcaccg caacaggcgc agcgtgcctt cgagccgttc ggtgtaacgc
     6961 cccagccaat atagattgtc cgcgacgcgg ctcggcaatt ccgccgcgga acgcccggag
     7021 cggaccaatt gtcctgacgg cgtcagcagc gaaaccatgt tgaccggccc gtcggaaatc
     7081 acccaggtgt ccttgctgcc gccgccgctt tgcatgctga caatcggttc ttccgcggca
     7141 gtcgagacgc ggctcaaccc gcccggcatg accgcgtacg aatcgcccat cgcggttgaa
     7201 taagcgcgca tcacaagcgg acgcggttcc agtttgccgt tgaaccaaac cggcgcagtg
     7261 gaaagcgtgg cgtgttcctg tgcgacaaat tcatcgggcc gctcgcgaat cgccgtgatc
     7321 aattcatcgc gttccttcgc cgccagcttc gcgccaaaat acggttcgcg cgccagcgaa
     7381 ccaaaggcgg gtttcacgac aaattcgtcc agatgatcaa tcacatattg acgttccttt
     7441 tcctggccgc accaccatgt cgccagcgaa ggaagtttca attcttcgcc cagcaattgg
     7501 cggcaaagac cgggcagaaa cgccatgaac gcggggcttt caatgacacc ggaaccgagc
     7561 gcgttggcaa tggccacatt cccggcgcgc gcggcttcga ccaatccggc cacgccgaga
     7621 aatgaatcgc cgcgcaattc gagcgggtcg cagaaggaat cgtccacgcg gcgcaggatg
     7681 acatccacct ggcgaagacc ttcgagggtc ttgatgaaga ccttgctgtc gcgcacggtg
     7741 aggtcgccgc cttcgatgag cgtgaagccg agataacgcg caagataatc gtgctcgaaa
     7801 taagtttcgt tatacgggcc gggggtgagc aacaccacgc tgggattgtc gcggttgtgt
     7861 ggcgcgaggc tgcgaagcgt gtcgcgatgg atgcggaaaa acgaggcgag acgctggaca
     7921 ttgcaatcgc ggaattcatc cggcaaaacg cgcgagagca cgatgcggtt ttccagcgtg
     7981 taacccgcgc cggacggggc ctgtgtgcgg tcggaaatca cccaccattg gccgtcgggc
     8041 gaacgcgtca aatcggtcgc gtgaaggtgc agataaattt gccgggggac gctcaagcca
     8101 tggcagggcc gcaggtaatg agggttcgca tagaccagcg cgggcggcaa aaaaccgtcc
     8161 ttcaataatt ttttcggacc gtaaaggtcg gtgaggatga ggttgaacaa ttttgcgcgt
     8221 tgaacaagtc cggcctcgat ctttgcccat tcggagggcg gcacgagcag cggcaccatg
     8281 tccaattccc agggccggtc catcccatgc gggtcgccat agacattgta agtgacgccg
     8341 tgttcg
//
LOCUS       DASZMM010000527         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_227026, whole genome shotgun sequence.
ACCESSION   DASZMM010000527 DASZMM010000000
VERSION     DASZMM010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227026"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2440
                     /locus_tag="VG754_12725"
     CDS             <1..2440
                     /locus_tag="VG754_12725"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HWF20126.1"
                     /translation="RMAIGWLDVVRFADTIGYHSDNPRNVWPYRDYVIKAFNENKPFD
                     QFTREQLAGDLLPNSTREQKVASAFNRLLLSTEEGGAQPKDYETRMLTDRVRAVSAVW
                     LGQTVGCAQCHNHKFDPIKQKDFYSLGAFFADLDEPIIGAREPGMLVPDKEQEAELNR
                     RDKEIELAKKQYDGPHPEWQAAFEDWQKEQLEIIAQEQLWKALTPVKAVAKNGGDLQV
                     NDDGFVLAVGKRADTDTYALSFTNGLKNLAGLRIEALPHDSLPSKGPGRADNGNFVLT
                     EVIAAVHRLDGTTNPISFVSAQADFEQSLGDAANPKLNWSAASVIDVNVKGDKKGWAI
                     LPQTNQAHQLVLGFPDTLTVQDGDTLVLKLKHDYPAHQLGHFRIGATTNSDAVKRPLR
                     TPIEKELADILLVPPDQRTDAQKRKLFARFKSIAPVAIQAAKKLDEMRQSRKDFEAAL
                     PHCIVSKTNATPRTVRVLARGNFLDDTGEIVQPALPAFLAGTNSDGRRLNRLDLANWL
                     VSRENPLTARVVMNRLWKQFFGLGLSKIVDDLGAQGESPPDQALLDWLACEFMDSGWD
                     MKHMVRLIVSSETYQQTSVAPKKLRTRDPQNRELAVQSRWRLDAELVRDNALAVSGLL
                     VDKIGGPSVKPYQPDGYWENLNFPARTYEASAGENQYRRGLYTWWQRSYLHPSLLAFD
                     APTREECAAERNRSNIPQQALVLLNDPTYVEASRALAVRMMKEGGADTKSRLVWTWRQ
                     VLSRAPRPDELETTESLLSKEIEEYRADPAAAEALLKTAQTPALKDADAAELAAWTNI
                     ARVILNLHETITRS"
     gene            2446..>3372
                     /locus_tag="VG754_12730"
     CDS             2446..>3372
                     /locus_tag="VG754_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF20127.1"
                     /translation="MKPHPELQQFLSQRQTRRTFLGRTAQGVGALALASLLNPSLLAA
                     AAGSRNDKWSGIVNPFDYPQKAKRVIWLSMAGGPSHLETFDYKPKLAEMDGKPMPDSF
                     TQGKQIAQLQGQKLICFGPQHGFKKFGRNGIEICELFPNIGSIVDDICFVRSMTTEAI
                     NHDPAHMFMNTGSQIAGRPSMGSWATYGLGSDAEDLPGFVVLTSLGQGGQNQPIAARQ
                     WSAGLLPSRFQGVLLRSKGDPVLYIGNPAGVTHAQQKDVVNAINTINAKYDTIVDDPE
                     ISTRIVQYEMAFKMQTSVPELMDIKSEGPKTLE"
BASE COUNT          792 a          967 c          948 g          665 t
ORIGIN      
        1 acggatggca atcggctggc tcgatgtcgt gcgttttgcc gacaccatcg gctatcattc
       61 cgacaatccg cgcaacgtgt ggccctatcg cgattacgtc atcaaggctt tcaacgaaaa
      121 caaaccgttc gaccaattca cgcgcgaaca acttgcgggc gacctgttgc ccaacagcac
      181 gcgcgaacaa aaggtcgcgt cggcattcaa ccgccttctt ctctcaaccg aggaaggcgg
      241 cgcgcaacca aaagattacg agacgcgcat gttgaccgac cgtgtgcggg ccgtcagcgc
      301 ggtttggctg ggccagaccg tcggttgcgc gcaatgccac aatcataaat tcgaccccat
      361 caagcaaaag gatttttatt cgctcggcgc gttttttgcc gaccttgacg agccaatcat
      421 cggcgcgcgc gaaccgggaa tgctggtgcc ggacaaggaa caggaagccg aactgaaccg
      481 gcgcgacaag gaaatcgaac tggcgaaaaa acaatacgat ggcccgcacc cggagtggca
      541 ggcggctttc gaggattggc agaaggaaca attggagatc atcgcgcagg agcagctttg
      601 gaaggcgttg acgcccgtca aagccgtggc gaaaaatggc ggcgaccttc aagtgaacga
      661 cgatggtttc gtcctcgcag tcgggaagag ggccgacacc gacacgtatg cgctctcgtt
      721 caccaatgga ttgaaaaatc ttgccggact tcgcatcgag gcgttgccgc atgattcgtt
      781 gccatcgaaa ggtcccggcc gggcggacaa tggaaatttc gttttgaccg aagtcatcgc
      841 ggcggtccac cggctcgatg gcacgacaaa tcctatttcg ttcgtttcag cgcaggcgga
      901 tttcgagcaa agccttggcg acgcggccaa tcccaagctg aattggagcg cggcttcggt
      961 gattgacgtg aacgtgaagg gcgacaaaaa aggctgggcg attttgccgc agacgaatca
     1021 agcgcatcaa ctcgtgctgg gattccccga tacgctcacg gtccaagacg gcgatacgct
     1081 cgtcctgaaa ctcaagcacg attatcccgc gcatcagctt ggccatttcc gcatcggcgc
     1141 aacgaccaat tccgatgctg tgaaacgtcc tttgcgcacg ccgattgaga aagaacttgc
     1201 ggacattctg ttggtccctc ccgaccaacg gaccgatgcg caaaaaagaa aacttttcgc
     1261 gcgtttcaaa agcatcgctc ccgttgcgat tcaggccgcg aaaaagctcg atgaaatgcg
     1321 ccagtcgaga aaagattttg aggcagcgct tccgcattgc atcgtttcaa agacgaacgc
     1381 cactccgcgg accgtgcgcg tgctggcgcg tggaaatttt ctcgatgaca ccggcgaaat
     1441 cgtccaaccc gccctgcccg cctttctcgc aggcaccaat tcggatggac gccgtctcaa
     1501 ccgtcttgat ctggcgaact ggctggtgtc gcgcgaaaat ccgctgaccg cgcgcgtggt
     1561 gatgaaccgg ctttggaaac aatttttcgg ccttggcctt tccaagatcg ttgacgatct
     1621 tggcgcgcaa ggcgaatcgc cgcctgacca ggcgttgctc gactggcttg cgtgtgagtt
     1681 catggacagc ggctgggaca tgaaacacat ggtgcgcctc atcgtgagca gcgagacata
     1741 ccagcaaact tcagtcgcgc cgaaaaaact tcgcacccgc gatccgcaga accgcgaact
     1801 tgccgtgcaa agccgctggc ggttggatgc cgaattggtc cgagacaacg cgctcgcggt
     1861 ttccgggttg ctggtggata aaatcggcgg gccaagcgtg aagccgtatc agcccgatgg
     1921 ttattgggag aacttgaatt ttcccgcgcg cacttacgaa gccagcgcgg gagaaaacca
     1981 atatcgccgc gggctttata cttggtggca acgctcctat ttgcatccga gtctgctggc
     2041 cttcgacgcg ccgacgcgcg aagaatgcgc cgctgaacgc aatcgttcca acattccgca
     2101 acaggcactt gtgcttctca atgatcccac ttacgtcgaa gcttcgcgcg cgctggccgt
     2161 ccgcatgatg aaggaaggcg gcgccgacac gaaatcgcgg ctcgtttgga catggcgtca
     2221 agtcctctcc cgcgcgccgc gcccggatga actcgaaacc acggaatctt tgctctcgaa
     2281 ggaaattgag gaatatcgcg ccgacccggc ggctgcggag gcattgctca aaaccgccca
     2341 aacgcccgcg ctgaaagacg ccgatgccgc ggaacttgcg gcatggacaa atattgcgcg
     2401 cgtcattttg aatttgcacg aaaccatcac gcgctcctga ccagaatgaa accgcatccg
     2461 gaacttcaac agtttctctc gcaacgacag acgcgccgga catttttggg acgcaccgcg
     2521 cagggcgttg gcgcgctggc gctcgcgtcg ttgttgaatc cttcgctcct cgccgccgca
     2581 gccggtagcc gcaatgacaa atggagtggc attgtcaatc cgttcgatta tccgcaaaag
     2641 gccaaacgtg tcatctggct cagcatggca ggcggaccgt cgcatctcga aacattcgat
     2701 tacaaaccca aactggctga aatggatggc aagccgatgc ctgattcttt cacgcaggga
     2761 aaacaaatcg cgcagttgca aggtcaaaaa ttgatttgct ttggtccgca acacggcttc
     2821 aaaaaatttg gccgcaacgg aatcgaaatc tgcgaattgt tcccgaacat cggttcgatt
     2881 gttgacgaca tctgctttgt gcgttccatg accacggaag ccatcaatca cgatccggcg
     2941 cacatgttca tgaacaccgg ctcgcagata gcgggccggc ccagcatggg ttcatgggca
     3001 acttacgggc tcggttcgga cgccgaagat ttgccgggat ttgtcgtgtt gacttcgctt
     3061 gggcaaggcg ggcagaacca accgattgcg gcgcggcaat ggtcggcggg tttgttgccc
     3121 agccgttttc aaggcgtcct tttgcgcagc aagggcgacc cggttcttta catcggcaac
     3181 ccggcaggag tgacccacgc gcaacaaaag gacgtggtaa atgccatcaa taccatcaac
     3241 gcgaaatacg acaccatcgt ggacgacccg gaaatttcca cgcgcatcgt gcaatacgaa
     3301 atggcgttca agatgcaaac gagcgttccg gaattgatgg acatcaaatc ggaaggtccg
     3361 aagacattgg aa
//
LOCUS       DASZMM010000528         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1866166, whole genome shotgun sequence.
ACCESSION   DASZMM010000528 DASZMM010000000
VERSION     DASZMM010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866166"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VG754_12735"
     CDS             complement(<1..1008)
                     /locus_tag="VG754_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWF20128.1"
                     /translation="MNSLTVPVEIADPINARILAISEDKIQGFQRDPLGEIAKLSGVE
                     LPVVIERIQAMLRAGTIRRVRQTLMATNLAPGALVAWQVPTDRLDSAFDYLFQNDPFS
                     GHVVIRSADPATPGSNYKLWTTVKVPQGFSMPKHCEFLLRQTGAEHYRLMPAKRLFAL
                     GVGHVRRRGMAPGSKADVPAEVMDVNVVQLNDQEWRVLTALKREFEPDEITRDIWQAR
                     ADEAGVSLEVFCELAEDLNRRKVIGRFSTFLEHVKPLASGERVTRFNALFHWAVPPGR
                     EIEAGREVGRHHILTHAYWREGGPEFRNVNVMGVAHGTDRELVLQHKAAIDEHLRSIN
                     IP"
     gene            1100..1540
                     /locus_tag="VG754_12740"
     CDS             1100..1540
                     /locus_tag="VG754_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF20129.1"
                     /translation="MTYRIATFEDCPLLAELNQQLIQDEGHRNSMTMPELERRMKEWL
                     SGDYTAVLFENGREAVAYALFRELSDEIYLRHLFVIPSRRRMGVGREAMEILRSHIWP
                     KDKRLTVSVLTSNKPAIAFWRTIGYCDYSMTLEILPTLNPQRTA"
     gene            1648..2316
                     /locus_tag="VG754_12745"
     CDS             1648..2316
                     /locus_tag="VG754_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF20130.1"
                     /translation="MRVLVVEDEKKTASFIRKALQAESFAVDVSHDGSEALLLACSTP
                     FDAIVLDIMLPGRDGLSVLRQLREQQNRTPVLLLSARGQVDERVEGLNAGADDYLPKP
                     FALAELIARVRSLGRRAGEPKSVILRIGDLSLDTITRQAQRGGKIFELTTREYRLLEF
                     LMRTPGRICGRMAILEKVWDYDFDPGTNLVDVYIKRLREKIDDGFEPKLLHTVRGVGY
                     VMKE"
     gene            2320..>2909
                     /locus_tag="VG754_12750"
     CDS             2320..>2909
                     /locus_tag="VG754_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWF20131.1"
                     /translation="MTIRARLTFWYAGILFVSVLLITGLSYHEFARERAKARPSNEES
                     DENDTPEDILQISMVCGIPAAIIALAGGWWLMRKAMAPVTALTDAASRINESNLNRQL
                     LRSGNGDELDRLTEVFNAMTARLDTSFNRIREFTVHASHELKTPLTVMHGELEIALQD
                     SALSANSREGLLSQLDEVQRLTKIVDGLSLLTKADAG"
BASE COUNT          634 a          815 c          836 g          624 t
ORIGIN      
        1 cgggatgttg atgctgcgca gatgctcgtc aattgcggcc ttatgctgaa ggacgagttc
       61 gcggtcagtt ccgtgcgcca cgcccatgac gttgacattg cgaaattccg gaccgccttc
      121 acgccagtac gcgtgcgtca atatatgatg ccggcccact tcccggccgg cttcgatttc
      181 ccggcccggc gggactgccc aatgaaacag cgcgttaaaa cgagtcacgc gttcgccgct
      241 ggcaagcggt ttcacgtgct ccaaaaacgt ggagaagcgt ccgatgacct tgcgccggtt
      301 caagtcctcg gcaagctcgc agaatacttc gagcgaaacg cccgcttcgt cggcgcgcgc
      361 ctgccagatg tcgcgtgtga tttcgtccgg ctcaaattcg cgtttcaacg cggtgagcac
      421 gcgccattct tgatcgttca attgcacgac gttcacgtcc atcacttcgg cgggaacgtc
      481 agccttgctg cccggcgcca tgccgcgacg gcggacatgt ccgacgccca acgcaaacaa
      541 gcgcttcgcg ggcatcaaac ggtaatgttc cgcgccggtt tggcgcaaaa gaaattcgca
      601 atgctttggc atcgagaaac cctgcggcac ctttaccgtt gtccaaagtt tgtagttcga
      661 gccgggcgtc gcgggatcgg cggagcggat gacgacgtga ccggaaaaag gatcgttttg
      721 gaacagataa tcgaaagcgg aatccagtcg gtcggtcggc acttgccacg cgacgagcgc
      781 gccgggagcg agattggtgg ccatcaaagt ttggcgaacg cggcggatgg ttccggcccg
      841 cagcatcgcc tggatgcgtt caatgaccac cggcaactcc accccggaca actttgcgat
      901 ttcgccgagc ggatcgcgtt ggaatccctg gattttatct tccgatatgg caaggatgcg
      961 ggcgttgatt ggatctgcga tctcaaccgg cacagtcaat gaattcattc gccaaaaccc
     1021 taccatatcg cggaaatttt caaaggacgg aaactggact ggacgccgcc aactccggac
     1081 attatcgtcg ccaaaaagga tgacttatcg catcgccact tttgaggatt gtccgctgct
     1141 cgccgaattg aatcagcagt tgattcagga tgaaggccac cgaaattcca tgaccatgcc
     1201 ggaactggaa cggcgcatga aagaatggct ttcgggggat tacaccgcgg tcctgttcga
     1261 gaacggtcgc gaagcggtgg cttatgcttt gttccgtgaa ttgtccgacg agatttattt
     1321 gcgccatttg tttgtcatcc cttcgcggcg cagaatggga gtgggaagag aagcaatgga
     1381 aattctccgc tcccacatct ggccgaaaga caaaagattg accgtgagcg tcctaacctc
     1441 aaacaaaccg gcaatcgctt tctggcggac catcggctat tgcgactatt cgatgaccct
     1501 tgaaatcctg ccgaccctga atccgcaaag gactgcatga caattttctc atccacggtt
     1561 catgtctgtt tcacgttccc gtgttgaaat gagggaatgt tgatgtcagc agttggcaaa
     1621 tggtcgcaac gtgttacaaa tagcctgatg cgtgtcctgg tcgttgaaga tgaaaagaaa
     1681 accgcctcgt tcatccgaaa ggcgctgcag gcggaaagct ttgccgtgga cgtttcccat
     1741 gacggctcgg aagcgctgtt gctggcatgt tcaacgccgt tcgatgccat cgtgctggac
     1801 atcatgctgc ccggacgcga tggattgagc gtgctgcggc aattgcgcga acagcaaaac
     1861 cgcacgccgg tgctgctgct gtccgcgcgc ggccaggtgg atgaacgcgt cgaaggattg
     1921 aacgccgggg ccgacgatta tctgccaaag cctttcgcgt tggctgagtt gatcgcgcgc
     1981 gtgcgttcgc tgggacgccg cgccggcgaa ccgaaatcgg tcatcttgcg catcggcgac
     2041 ctctcgctcg acaccatcac acgccaggcg caacgcggcg gaaaaatttt tgaactcacc
     2101 acgcgcgaat accggctttt ggaatttctg atgcgaacgc cgggccggat ttgcgggcgc
     2161 atggcaattc tcgaaaaggt ttgggactac gatttcgatc cgggcacgaa tcttgttgat
     2221 gtgtacatca aacggttgcg cgaaaaaatt gacgatggtt tcgagcccaa attgctgcat
     2281 accgtgcgcg gcgtcggtta cgtgatgaag gaatgaacca tgaccatccg cgcgcgcctc
     2341 accttttggt atgccgggat tttgttcgtc tcggtgctgt tgatcaccgg gctgtcgtat
     2401 catgaatttg cgcgggaacg cgcaaaggcg cggccttcca atgaggaatc ggacgagaat
     2461 gacacgcctg aagacatcct gcaaatctcg atggtctgcg gcattcctgc ggcaatcatc
     2521 gcgctggcag gcggttggtg gctgatgcgc aaagcgatgg ctcccgtcac cgcgcttact
     2581 gacgccgcgt cgcggatcaa cgaaagcaat ctcaacaggc aactgcttcg cagcggcaat
     2641 ggcgacgaac tggaccgtct caccgaggtg ttcaacgcca tgaccgcgcg gctggacact
     2701 tccttcaacc gcatccgcga attcactgtt cacgcttcgc acgaactcaa gacgccgctg
     2761 actgtgatgc atggcgaact tgaaatcgcg ttgcaagaca gcgccctttc cgcaaacagc
     2821 cgcgaaggtt tgctcagcca gttggatgaa gtccagcggc tcacgaaaat tgtggatggc
     2881 ctttctttgc tcacaaaagc ggacgcggg
//
LOCUS       DASZMM010000529         3945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3528065, whole genome shotgun sequence.
ACCESSION   DASZMM010000529 DASZMM010000000
VERSION     DASZMM010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3528065"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3718)
                     /locus_tag="VG754_12755"
     CDS             complement(<1..3718)
                     /locus_tag="VG754_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20132.1"
                     /translation="MKTFLAFCCALFYTACVHAAGTSDGSVTLRDFHLVGDLHDDRAA
                     FTLTATAHVEDSHGGSLDLLSGTVALTEIGQHSKWHVRVEQNRFALTFDRGGDFPIKI
                     KFTAAVRLNDGWNTVDFRVAPSALQPIVLEGLAADTQFQFAGAARPEHVGNDFTSYLP
                     ADGSVKLAWKEARREAEGKLFYAAESLAQITVRPGLMRQTALLDFKVMQGELSQVALV
                     LRGDGEVTRVQGDQVLAWNVEPAQNGERRLVVRLNQPQKDQFALQVQMQTPLGAFPQV
                     ADAMQLRPDGATRYAGWIRVVNEGAVRLEVTQASGLSQISPEQFPESDATKALFAASG
                     SQRFAYRFSGADFALRIQADQIFPELSVSQVTSYNLGENELAIDSEIELDVREAPLRE
                     LLLHVPKGYAIARLTASGLTDYFLTEPQDQTDAELRLVYAEPLSGRQLIELRLERNQA
                     LGAASWMLPHIEVAKAKSVRGHIAVSADAGFRLTPERTQSLVEIATAFFPKKIAGIQT
                     AFRLNDASWEATLHVERLPQTVQADVLHLFSIGEGIAYGSSVMNYTVSGAPMSAFRVQ
                     LSEEYFNVEFTGKDVRNWQKTSDGYLVQLHTPVSGAYTLLATYERPFKSQGETLAFTG
                     ARPVDAQNEQGHTLVISAYQFQVKPAEISPGLLSLETGEVPPEYRLLFDAPILAAYRY
                     TSRPFNLRLTLTPRIQHDSLSQVVDRATLTTRISKEGQVLTDARYFLKNRGNPNFRFT
                     LPEGSTNLWSATVNGTPVVPVLDGKANLIPLPQRADPNAVLTVELKLAARSKDPGRVT
                     VAAPTLLDAPILLGEWRLEPDTGQRLVYRKGSLAPVNGVPDISGFAGLARVFSGQEPG
                     HAIQLLVAVILLTGIAIGLWHWTLRNRFGRFSARHLLGTGVGLLAFGMAMLALYNLGS
                     LVPHDDRVAPADATFLAPVQQAGSSLSIEVANVPLKASALGAVAPAWPALLALLVWFY
                     GGTRNSAGTKTMCGVLGWTLLAWAALRIPNGAPVFCLVLIVFLLVRVAIPAMRRLLQL
                     PRQASPDAAGAPAIAAFLLGALLWLSFGQTVSARPKEYVPAQKESPLAESVIQQIRVE
                     DKFAIATAKIHWQAEKGQRLPVLFEPAVLTHIIYPTRALKLEEVVSNGHRAQELVAHE
                     NGAFDIEVQYELQVSKTGDGSSFILPTQNGLINQIKLTLANLDVDVASPQAVSIQRDT
                     AGSNTVATLVLSPLGESQIGWRPRSRDVKREKPVF"
BASE COUNT          855 a         1198 c         1134 g          758 t
ORIGIN      
        1 aaaacaccgg tttttcgcgc ttcacatcgc ggctgcgcgg acgccagcca atctgggatt
       61 cgccaagcgg cgacaacaca agcgtcgcga cggtattgct gcctgccgtg tcgcgctgga
      121 tggaaacggc ctgcggcgag gccacgtcca cgtcaaggtt ggcgagcgtg agtttgattt
      181 ggttgatgag gccgttttgc gtcggcaaga tgaagctgct gccgtcgccc gttttcgata
      241 cttgcaattc gtattgcact tcgatgtcga acgcgccgtt ttcatgggcg accaattcct
      301 gcgcgcgatg gccgttggaa acgacttcct ccagcttgag tgcgcgcgtg ggataaatga
      361 tgtgcgtcaa caccgccggc tcgaacaaca ccggcaacct ttgccccttc tccgcctgcc
      421 aatggatctt tgccgtggca atcgcgaatt tatcttccac tcggatttgt tggatgaccg
      481 attccgcgag cggcgattct ttctgcgctg gcacgtattc ttttgggcgg gccgaaaccg
      541 tttgcccgaa gctcaaccaa agcaatgctc ccaacaaaaa cgccgcgatc gcaggggctc
      601 ctgccgcgtc cgggcttgct tggcgcggca gttgcaacag ccggcgcatt gctggaatcg
      661 ccacgcgcac cagcaggaac acgatcaaca caaggcaaaa tacaggagcg ccattcggga
      721 tccgcaacgc tgcccatgcg agcagcgtcc agccaagaac gccgcacatc gtcttggttc
      781 ccgcgctgtt cctcgtccca ccgtaaaacc aaaccagcaa cgccagcaaa gcaggccatg
      841 cgggagcgac agcgccgagc gcggatgcct tcaatggaac gttcgccact tcgatgctca
      901 aactgctgcc cgcctgttgc accggagcaa gaaatgttgc gtccgccggg gcgacgcgat
      961 catcatgcgg cacgaggctg ccaagattgt agagcgcgag cattgccatc ccaaacgcga
     1021 gcagaccgac gccggtcccc aaaagatggc gcgcgctaaa gcggccaaaa cgatttcgaa
     1081 gtgtccagtg ccagagaccg atggcaattc ccgtcagcaa aatcaccgcg accagcaatt
     1141 ggatggcatg tcccggttct tggccggaaa aaacgcgcgc caacccggca aatccggaaa
     1201 tgtccggcac gccattcact ggcgcaagcg accctttgcg ataaaccaga cgctggccgg
     1261 tatcgggttc gagccgccat tcacccaaca aaatgggcgc atcgagcaga gttggcgcgg
     1321 cgacagtcac tctgccgggg tctttcgagc gcgcagccag tttcaattca accgtgagaa
     1381 cagcattcgg atcggcccgc tgcggaagcg gaatcaggtt cgccttgccg tcgagcacag
     1441 ggacgactgg cgtgccgttg actgtcgccg accacaaatt cgtcgagcct tccggcaagg
     1501 tgaaacggaa atttggattg ccgcgatttt tcaaaaaata acgcgcgtcg gtcagcacct
     1561 ggccctcctt ggaaatgcgc gtggtgagcg tggcgcggtc cacgacctgg ctgagcgaat
     1621 catgctggat gcgcggcgtg agcgtgagcc gcaaattgaa cgggcgcgaa gtgtaacgat
     1681 acgccgcaag gatcggcgcg tcgaaaagca gccggtattc cggcgggact tcgccggttt
     1741 cgagcgagag caagccgggc gaaatctcgg cgggcttgac ctggaattga tacgcgctga
     1801 tgaccagcgt gtggccctgt tcgttttgcg cgtcaaccgg tcgcgcgccg gtgaacgcaa
     1861 gtgtttcgcc ctgcgacttg aacgggcgct cgtaagtcgc cagcagcgtg tatgcgcccg
     1921 acaccggcgt gtgcaattgc acgagataac catcactggt tttctgccag ttgcgaacgt
     1981 ccttgccggt gaactcgaca ttgaaatatt cctccgacaa ttggacgcga aaagccgaca
     2041 tcggcgcgcc ggaaaccgtg taattcatca cgctgctgcc gtaagcgatc ccttcgccga
     2101 tggaaaaaag atgcagcacg tccgcctgca ccgtctgcgg caaacgctcc acatgcaggg
     2161 tcgcttccca cgaggcgtca ttgagccgga acgcggtttg gatgccggca attttcttcg
     2221 gaaaaaacgc ggtcgcaatc tccacaagcg attgtgtgcg ttccggcgtc aatcggaaac
     2281 cggcatcagc cgacaccgcg atatgcccgc gcaccgattt cgctttcgca acttcgatgt
     2341 gtggcaacat ccagcttgcc gcgccaagcg cctggttgcg ttccaaacgc agttcgatga
     2401 gctgacggcc cgacaacggc tcggcataaa caaggcgcaa ttcggcgtca gtttggtctt
     2461 gcggttcggt gagaaaataa tcggtcaacc cggacgcagt gagccgcgcg atggcatagc
     2521 ctttcggcac atgcagcaac aattcgcgca acggcgcttc gcgcacgtcc agttcaatct
     2581 cggaatcaat cgcaagctcg ttctcgccaa gattgtaaga agtcacctgc gagacgctca
     2641 gttcgggaaa aatctggtcc gcctggatgc gcaacgcaaa atccgcgccg gaaaaacgat
     2701 aggcaaaccg ttgcgaacct gatgcggcaa aaagcgcctt ggtcgcgtca ctttccggaa
     2761 attgctccgg cgaaatctgc gagaggccgc tcgcttgcgt cacttcgagc cgcaccgcgc
     2821 cttcattcac gacgcgaatc cagcccgcgt aacgcgtcgc gccgtccgga cgcaattgca
     2881 tcgcatccgc cacttgcggg aatgcgccga gcggcgtttg catctggact tgcagcgcga
     2941 actggtcttt ctgcggctga ttaaggcgca cgaccaaccg ccgttcgccg ttttgcgccg
     3001 gctcgacatt ccacgcaagc acctggtcgc cctgcacgcg cgtgacctcg ccgtcgccgc
     3061 gcaacacgag cgccacctgg ctcaactcgc cctgcatcac cttgaaatcg agcaacgcgg
     3121 tctgccgcat caaacccgga cgcacggtga tttgcgcaag cgattcggct gcgtagaaaa
     3181 gtttgccctc cgcttcacgc cgcgcttcct tccacgccag tttcaccgaa ccatccgccg
     3241 gcaaataact cgtaaagtcg ttgccaacgt gttccggacg cgctgcgccg gcgaattgaa
     3301 attgcgtgtc cgccgccagc ccttcgagaa cgatcggttg caacgcgctg ggtgcgacgc
     3361 gaaagtccac cgtgttccag ccgtcattga ggcgcactgc ggcggtgaat ttgattttga
     3421 tgggaaaatc tccaccgcga tcgaacgtca gcgcgaaacg gttttgctcc acgcgcacat
     3481 gccatttcga atgctggccg atttccgtca gcgcgacggt cccggagagc aaatcaagcg
     3541 aaccgccgtg ggaatcttcg acatgcgccg ttgctgtgag cgtgaacgcg gcgcggtcgt
     3601 cgtgcaggtc gccgacgagg tggaagtcgc gcagtgtgac gctgccatcg gaagtcccgg
     3661 cggcatgaac acaagccgta taaaaaagag cgcaacagaa agcgagaaag gttttcatta
     3721 tatttcagtt aggtttggac gaaggtctca ttgaataaac actgcttgag aaataagggg
     3781 atgaaaggcg gcaggccgtc ttcaccgtag cgcagagttt tactctgctg tatcgcagag
     3841 ttgcactctg cggagcggct aagaattcca cacgctttga acctgccaac accctgccga
     3901 ctgcaagtcg gcgatacagc agaatgcaat tctgcgctac aagct
//
LOCUS       DASZMM010000530         9243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3257949, whole genome shotgun sequence.
ACCESSION   DASZMM010000530 DASZMM010000000
VERSION     DASZMM010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257949"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2504)
                     /locus_tag="VG754_12760"
     CDS             complement(<1..2504)
                     /locus_tag="VG754_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HWF20133.1"
                     /translation="MKNPQTDGVIRERFTKLFEFLKAFSDLQYPLVRDIDLQPRTLWL
                     GNIFGHSGVELYRDETQSPETTTDNSIVFRVTRPTLSLCPPPPAGLKGWLKPDWQKVD
                     GKAEFLPSRNMPGFEGRKRVERFDEDPIRVEGFREWVREREQWVKNERPLKRASALFQ
                     EVYEWFGMLEREGERIELLLGDGLLCWQHETGEFKHPVLLQKLELEFHPEKREPQFIF
                     RKREHPPELYREFLHSLPGSNSQQIVRCDAEMREAEFSPLGGQNTAEFFRRLIQSVFP
                     AGGEFCEPGQKPSGSVPYIERRPVIFMRQRRIGPANVFDLVLEDIEQRLLAGEPFSTA
                     LMQVLGLIEQSPQPSDLEARAPTPGNEDADVLLSKPANREQLEIARHLARRDCVLVQG
                     PPGTGKTHTIANLLGHLLAQGKRVLVTAHTPKALRILREKVVEPLQPLCVSVLQNDKK
                     SHEELQQSVRQIHVRLSQDDRLLEQDAARLRDERTAVLNELRDARQRLLEARRDEIRE
                     VTFAATAVRTIEAAKLVKAGAGKNDWIPSPVTLGEALPLSEEEIIALYQSNDRISADD
                     ERELEISRPDSASFPSPLEFKRTVEEIQSLCEEDLRFGAQFWVETGEPQDLSEFERML
                     EMTGNAIEFFKDSAAWQLDAIQAGRDGEPARLTWDALADFIEQSWQEIHQCHALVMEH
                     GPSITDSRPARVLLPMLDEIIKFVSEGGRFNALTKLTKRHWLAFMETIRINDHAPDFS
                     SMDQLRAMRAQLRIAQLREEMCGRWARMMTSGGAPSVEQLGNRPEQACRGFVSQIRAC
                     LEWHRTAWQPLEAEFHRLGFNWSAYLESTAPGVGDHA"
     gene            complement(2722..3663)
                     /locus_tag="VG754_12765"
     CDS             complement(2722..3663)
                     /locus_tag="VG754_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HWF20134.1"
                     /translation="MSESSQFLIKHGLPFLFAAIFVEQLGVPLPALPWLLIAGALSAT
                     GQFNPLLGVAATVLACLPADAVWFYLGRYRGNQVLHFLCRITLEPDSCVRRTQNIFFR
                     HGMRGVIAAKFLPGFGTLIPPLAGMSGLSAARFLMMDAIGSISYGAVYIGLGFLFSKQ
                     IEQIVAMISNTGGNTVALLIGLAAGYIGFKFWRRQRLLRKLRMARITASELRQKQAAG
                     EELFILDLRSREEVELDPSFIDGAIHFEMAEVESRIHEIPRDRDVILYCACPNEVTSA
                     RVALLLHRKGIARVRPLLGGIDAWRELNNSVKPESQI"
     gene            complement(3679..5115)
                     /locus_tag="VG754_12770"
     CDS             complement(3679..5115)
                     /locus_tag="VG754_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF20135.1"
                     /translation="MDTATLQQVRATPLFANLTEEQLDCIKMGEVIETPIGTVIATDG
                     KRTGFFYVILDGEVRITRTYDRQSILMGTGTAGDFLGEIMLLLDIPWLSTVRVSKAAR
                     LFRLDEEQFWKLLATCRSVAREIFRETANRLRNVEGYTQQREKLASLGTMAAGLAHEL
                     NNPAAAARRAAAHLQETTDRLQCFLCDLTQHLEHTHWEHLLAASDEAFERLKKISSLD
                     HLERSDREDALATWLTGHRVPKAWELAPTFVTAGLDVDWLNTTIQNIPPASWVGALCW
                     LEARLSLTMLLSQVEQSTTRIAELVKAVKSYSHMDESPMQEVDIHDGIESTLTMLSHK
                     LKKVTVVRSFDRALPRFMAYGSELNQVWTNLLDNAIDAVDGAGKICVSTTFEDNQAVV
                     EIVDNGPGIPQDVQTRMFQPFFTTKSVGSGTGLGLVISNRIVADRHGGEIEFESKPGE
                     TRFRVRIPLIHRETHPGVPADLQINRAL"
     gene            complement(5118..6779)
                     /locus_tag="VG754_12775"
     CDS             complement(5118..6779)
                     /locus_tag="VG754_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF20136.1"
                     /translation="MPKPVIWTIDDDPDVLRAVERDLRRQYGDRYKIIPADSGVTALE
                     SVKQLKLRNETVALFLVDQRMPHMSGVEFLEQAIKIFPDAKRALLTAYADTDAAIRAI
                     NNVQVDHWLMKPWDPPEERLYGVVDDMLDDWQASFRPAFEGVRVVGHQWSPHSSEIRD
                     FLGRNFVPHQWLNVETDEAAKQLIETAGADCSSLPLVVFADGSVLKNPLVGDVARKLG
                     LRTKAEFPFYDLVVVGAGPAGLAAAVYGAADGLHTLLIEREAPGGQAGRSSRIENYLG
                     FPVGLSGNDLARRAVAQARRFGAEILSPQEVNSVRIEGPTRVLTLADGGEIGCRALLI
                     ATGIAFRKLDVPGLDKFSGAGVYYYAPMSEAFSHRDGDIYIVGGANSAGQAAMYFSKI
                     ARQVTMLVRGNSLSATMSQYLENQIAATKNISVRLNSSVIAVNGAERCETITIQNNKT
                     CTQETMPASALLIYAGAVPRTDWLEGVVERDTQGYLISGQHLVREGKLPAGWPAGREP
                     LYLETSVPGIFVAGDVRHRSAKGVTSGVGEGAMAVKLVHEYLGLQ"
     gene            complement(6781..7374)
                     /locus_tag="VG754_12780"
     CDS             complement(6781..7374)
                     /locus_tag="VG754_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWF20137.1"
                     /translation="MNDGLFHHALAVPVSGDDHVQGPDSAAVTLVLYGDYQCPYTAAL
                     HQVVTRLQVRFGENLRYVFRHFPLFAKHPKAQLAAETAEAASAQGKFWQMHAYLFQAQ
                     YDFDRERLGAACSLLGLDYVRLDSDISNHVHLEHIQKDVAGAKSSGVASTPTLFVNGF
                     MYDADDDFETLSRNVESLVPMTNEPKRRPFWRGLFGN"
     gene            complement(7504..9021)
                     /locus_tag="VG754_12785"
     CDS             complement(7504..9021)
                     /locus_tag="VG754_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF20138.1"
                     /translation="MPRHFVIQLIIGLTVALLLLPALLLRLRRGRRTIFKKLLSAFCW
                     FVLALDCAAWVILFLLHLGVVELEWRGWYAPVVTWKKTKPDFAAVEQNRLQQSKLGPA
                     PAINSTIHANWHGFRGPHQNGEYDERPILTNWPDTGLKLLWHQPCGGGYSSFALADDR
                     AFTLEQRRDYEVLAAYDLATGRELWTNGWTAKFTEYHSDEGPRSTPAYDDGKDYALGA
                     TGELCCVNATNGTVVWSKNIMTENKAALPDYGIAASPLIVDEKIILQPDAYHGKSVVC
                     YDKRDGKILWHALDLPMGYATPMLETIGGERQVIVCGRPDVIGLRLDDGAERWRIHWP
                     IINHERPITQPLMLNTNSLMLSAAYMTGSGAYEIDRSGDAYETHELWRTKSLKAKFAS
                     AVFWQGYIYGLDEDILACVDAQTGERKWKDGRYGYGQLLLASGHLIVLCANGDLALVK
                     ATPEKLTEIARFPALHGKSWNAPAIGDGRILLRNGAEMACYEIAPPSTPATESKK"
     gene            9010..>9243
                     /locus_tag="VG754_12790"
     CDS             9010..>9243
                     /locus_tag="VG754_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20139.1"
                     /translation="MSGHRSIRFSGHRNFAGERADASTFLFLRGPIKTFQPARFQVNV
                     QQRMKENDSNAGRFDRRIFLKASGMALGLAGLAS"
BASE COUNT         2031 a         2605 c         2598 g         2009 t
ORIGIN      
        1 gcatgatcac ccactcccgg ggcggtggac tccaaatagg cggaccaatt gaagccaaga
       61 cggtgaaact ccgcctccaa aggctgccac gcggtccgat gccattcgag acacgcacgg
      121 atctggctga caaatccacg gcaggcttgt tcgggacggt tgccgagttg ctcaacggag
      181 ggagcgccgc ccgaagtcat catgcgcgcc cagcgcccgc acatttcttc acgaagttgc
      241 gcgatgcgca gttgagcgcg catggcacgc aattgatcca tggaactgaa gtccggcgca
      301 tggtcattga tgcggatggt ttccatgaag gccagccaat ggcgtttggt caattttgtc
      361 aacgcgttga atcgtccgcc ttcggaaacg aacttgatga tctcatcaag catcggcagc
      421 aggacgcgcg cgggccgcga gtcggttatg cttggcccat gctccatcac gagcgcgtgg
      481 cattgatgaa tttcctgcca ggattgctcg atgaaatcgg cgagcgcatc ccaggtcaag
      541 cgggcaggtt caccgtcccg gccggcctgg atcgcatcca gttgccatgc ggcgctgtcc
      601 ttgaaaaatt caatcgcgtt gcccgtcatt tccagcattc gctcgaattc agacaagtct
      661 tgcggttcac cggtctcaac ccaaaattgc gcgccaaatc gcaagtcctc ctcgcagagc
      721 gactgtatct cctcgacagt gcgcttaaat tccaacgggc ttggaaacga tgccgaatcg
      781 gggcgcgaga tttcaagttc gcgctcgtcg tccgcggata tacggtcgtt cgactgatag
      841 agagcaatga tctcctcttc ggaaagcggc aaggcttcgc ccaaggtgac cgggctggga
      901 atccaatcat tcttgcccgc gccagccttg accaacttgg cggcctcaat ggtccggacg
      961 gccgtggccg caaaggtcac ttcacgaatc tcgtcccggc gcgcctcaag caaccgttga
     1021 cgcgcgtctc gcaattcatt tagcacggcg gtgcgttcat cacgcaaccg cgcggcatcc
     1081 tgctccaaca gccggtcatc ctgtgaaagc cggacgtgaa tctgccgcac cgattgctgg
     1141 agttcctcat gacttttctt gtcattttga agaacactga cgcaaagtgg ttgcagtggt
     1201 tcaacaactt tttcgcgcaa gatgcgcagg gctttgggag tgtgggccgt gaccaggacg
     1261 cgtttgccct gagccagcaa gtgacccagc aaattggcga tggtatgtgt cttgcccgtg
     1321 cccggcgggc cttgaacgag cacgcagtcg cgacgcgcca gatggcgggc gatttccaat
     1381 tgttcgcgat tggccggctt gctgagcagc acatcggcgt cctcgttgcc cggagttggc
     1441 gcgcgcgctt ccaagtctga cggctgggga gattgctcga tcaggccaag aacctgcatg
     1501 agcgcggtcg agaaaggctc tcctgccagc aggcgttgct caatatcttc aagcaccaga
     1561 tcaaaaacat tggccggtcc gattcggcgt tgccgcatga aaataaccgg tcgccgctcg
     1621 atatagggga cagagcccga gggtttctgt ccgggttcgc agaactcacc gcctgcggga
     1681 aagacacttt ggatcagccg ccggaaaaac tctgcggtgt tttgtccgcc caacggagaa
     1741 aactcggctt ctctcatttc cgcatcgcag cggacgattt gctgtgaatt gctgcccggc
     1801 agggagtgga ggaattcacg gtaaagttcc ggaggatgct cgcgcttgcg aaagatgaac
     1861 tgcggctcgc gcttttccgg atgaaattcc agctccagct tttgcagcag gacggggtgc
     1921 ttgaattctc ctgtttcgtg ctgccagcaa agcaagccgt cgcccaggag caattcgatc
     1981 cgctcccctt cgcgttcgag cattccgaac cattcataaa cttcctggaa caaggccgag
     2041 gcccgtttca gcggacgttc attcttgacc cactgttccc gctcgcgaac ccattcccga
     2101 aatccctcaa cccgtatggg atcctcgtca aagcgttcca ctcgcttgcg gccttcaaac
     2161 ccgggcatgt tgcggctcgg caggaattcg gctttgccat cgaccttttg ccaatcgggc
     2221 ttgagccagc ccttcagccc ggcaggcggc ggagggcaga gcgaaagtgt gggccgcgtg
     2281 acgcgaaaaa cgatgctgtt gtccgtcgtg gtttccggcg attgcgtctc atctcggtac
     2341 aattccacgc ccgagtgccc aaaaatgtta cccagccaaa gcgtcctggg ctgcaagtca
     2401 atatcgcgga ccaatggata ttgcaggtcg ctaaaggctt taagaaattc aaaaagcttc
     2461 gtgaaacgtt cccgaatgac gccgtcagtt tgtggatttt tcatgggggt gggtgctaac
     2521 agacagttgc gagattggcg aaagataaag cgatatcagg caaattacaa atttgaaatc
     2581 cgcgccacaa cgatgctggg tgggagcata acacctgcca gaaattgaag cagaagattg
     2641 cctgcggttc cattcgtgct cggattcagg ctgccaagct gctggcgatt gccgggagcg
     2701 cgtttccttc cgtctggctg tctagatttg actttcgggc ttcaccgaat tgttcagctc
     2761 acgccaggca tctatcccgc ccaataaagg ccggacgcgc gcaatgcctt ttcggtgcag
     2821 caacaacgca acgcgagcgc tcgtcacttc atttgggcag gcgcagtaga ggatgacatc
     2881 gcggtcgcgt ggaatttcat ggatgcggga ttccacctcg gccatctcaa agtgaatcgc
     2941 gccatcaatg aacgaagggt cgagttcgac ttcctcgcgc gaccggaggt cgagaatgaa
     3001 caactcctcg ccagcggctt gtttttgacg caattcagac gccgtgatgc gggccatgcg
     3061 caacttgcgc aacaatcttt gccgccgcca aaatttgaag ccgatgtagc cggcggccaa
     3121 tccgatcaac aaggcgacag tattgccgcc ggtgtttgaa atcatcgcga caatttgttc
     3181 gatctgcttg ctgaacagaa aacccagccc gatgtaaacg gctccgtaac ttatggaacc
     3241 gatggcgtcc atcatcaaaa accgcgccgc gctgaggccc gacatgccgg caaggggtgg
     3301 aatcaacgtg ccaaaaccgg gcaggaattt tgccgcgatc acgccgcgca ttccatgacg
     3361 gaaaaaaata ttctgtgtgc ggcggacaca ggaatccggt tccagcgtga tgcgacacag
     3421 gaagtgcaag acctgattgc cccgataccg gcccagataa aaccacacgg catctgccgg
     3481 caggcaggca agcactgtgg cggcgactcc cagcagcgga ttgaattggc ctgtcgcaga
     3541 gagcgcgccc gcgatcaaca accacggcaa tgccggcaat gggacgccaa gttgttccac
     3601 aaaaatggcg gcaaacaaaa atggcaatcc atgtttgatg agaaattgac tgctttcaga
     3661 catgcgtttg cgggaaaatt ataaagctcg attgatttga agatcggcgg gcacaccggg
     3721 atgagtctcg cggtggatta acggaatgcg gaccctgaaa cgcgtttcgc cgggcttcga
     3781 ctcaaactca atctcgccac cgtgacggtc tgcgacaatg cgattgctga tgaccaatcc
     3841 aaggccggtc ccggagccga cggatttcgt ggtaaaaaaa ggttgaaaca tgcgtgtctg
     3901 aacgtcctgc gggatgccgg gaccattgtc cacgatttcc acaaccgcct gattgtcttc
     3961 aaaggtggtg ctgacgcata ttttgccggc gccatcaaca gcgtcgatcg cgttgtccag
     4021 cagatttgtc caaacttggt taagctcgct gccgtaagcc atgaaacgcg gcaacgcgcg
     4081 atcaaatgag cggacgacag tgaccttttt caacttgtgg ctgagcatgg tcagtgtgct
     4141 ttcgatgccg tcatggatgt ccacctcctg catgggagat tcgtccatgt gcgaatagga
     4201 tttgaccgct ttgacgagct cggcgatgcg tgtcgtgctc tgctcgacct ggcttaacag
     4261 catcgtcaag ctcaagcgcg cttccagcca gcacaaggca ccgacccaac ttgcaggagg
     4321 aatattttga atcgtggtgt tgagccagtc cacgtccaat cccgcggtca cgaacgttgg
     4381 cgcgagttcc caagctttgg ggacgcgatg gcccgttagc caggtggcaa gtgcgtcctc
     4441 gcgatcgctt cgctccagat gatccagcga agagattttt ttgagacgtt caaaagcctc
     4501 gtcggaggcg gcaagaagat gctcccaatg agtgtgctca aggtgttggg tgagatcaca
     4561 cagaaagcat tgaagtctgt ccgtcgtttc ctgcaaatgc gctgctgcgc ggcgggccgc
     4621 tgcggcgggg ttgttcaatt cgtgcgccaa tcccgctgcc atcgtgccga gcgaggcgag
     4681 tttttcccgt tgctgcgtgt agccttcgac gttgcgcagg cgattggcag tctcgcgaaa
     4741 aatttcacgg gcaacggaac ggcaagtcgc caataatttc cagaactgtt cttcatcgag
     4801 ccggaacaac cgggctgcct ttgacacccg gacggttgaa agccagggaa tgtccagcag
     4861 aagcatgatt tctcccagga aatctcccgc cgtgccggtc cccatcaaaa tcgattgacg
     4921 gtcgtaagtc cgggtgatgc gcacttctcc atcgagtatg acataaaaga aacctgtccg
     4981 cttcccgtcg gtggcgatca ccgtcccaat cggcgtttca atgacttcgc ccattttgat
     5041 gcagtccaat tgttcctcgg tcaggttcgc aaacaatgga gtcgcgcgga cttgttggag
     5101 tgttgccgtg tccatggtca ttgcaggccg agatattcgt gaacgagttt caccgccatt
     5161 gcgccttcgc ccacgccgga agtgactccc ttggcggagc gatggcggac atcgccggca
     5221 acaaaaatac cggggacgct tgtctcaaga tagagcggct cgcgaccggc gggccagcct
     5281 gccggaagct tgccctcgcg cacgaggtgc tggccggaaa tgagatagcc ttgcgtatcg
     5341 cgttccacga caccttcaag ccagtcggtg cgggggactg cgcctgcata aatgagcaac
     5401 gcgcttgcgg gcatcgtttc ctgcgtgcag gtcttgttgt tttgaatggt gatggtttcg
     5461 catcgttcag cgccgttcac tgcaatgacg ctggaattga gccgcaccga aatatttttc
     5521 gtggcggcaa tttggttttc caggtattgc gacattgtgg ctgaaagaga attgccgcgg
     5581 acaagcatgg tcacttgacg ggcaattttg gaaaaataca tcgcagcctg tcctgcggaa
     5641 ttcgcgccgc cgacgatata aatgtcgccg tcccgatgcg aaaacgcctc ggacatgggc
     5701 gcatagtaat aaacacccgc gccgctgaac ttatcaagcc cgggcacgtc gagtttcctg
     5761 aacgcgatgc cggtcgcaat caataacgcg cggcagccga tttccccgcc gtccgccagg
     5821 gtcaacacgc gggtcggccc ttcgatgcgc acgctgttga cttcctgcgg tgataaaatt
     5881 tccgcgccga agcgccgagc ttgtgcgacc gcgcggcgtg ccagatcatt gccgctcaat
     5941 ccaacgggaa aaccgagata attttcgatg cgcgaactgc gtccggcttg tccgcccgga
     6001 gcttcccgtt cgatcaggag cgtatgaagg ccatccgccg ctccatagac agcggcggcc
     6061 aatcccgccg gacccgcgcc gaccaccacc aaatcgtaga agggaaattc cgccttggta
     6121 cgcaaaccca gtttacgcgc cacatcaccc acgagaggat tcttaaggac ggagccatcg
     6181 gcaaacacga ccagcggcag cgacgaacaa tcggctccgg cagtttcaat gagttgtttc
     6241 gctgcttcat ccgtttcgac attgagccat tgatgcggca cgaaatttcg tccgagaaaa
     6301 tcgcggatct cgctcgaatg cggcgaccat tgatgcccga caactctcac gccttcaaac
     6361 gccggtcgga acgaagcctg ccaatcgtcc agcatgtcat ccacgactcc ataaaggcgt
     6421 tcttcaggcg ggtcccacgg cttcatcaac cagtggtcca cctggacatt gttgatggcg
     6481 cgaatggccg catccgtgtc ggcataagcg gtcaacaacg cgcgctttgc atccggaaaa
     6541 attttgatgg cctgctccaa aaactcgacg cccgacatgt gcggcattct ttggtccacc
     6601 aaaaaaagcg ccacggtttc attgcgcagc ttcaattgtt tcaccgattc cagcgccgta
     6661 acgccggaat ctgccgggat gattttgtaa cggtcgccgt attggcggcg caggtcgcgc
     6721 tcaacggcgc gcaaaacgtc cgggtcatca tcgatggtcc atatcacggg tttgggcata
     6781 tcaatttccg aataatccgc gccagaaagg ccggcgcttg ggctcgttcg tcattggaac
     6841 aagcgattca acatttcgcg aaagcgtctc gaagtcatcg tcggcatcat acatgaaccc
     6901 gttgacaaag agcgtcggcg tgctggcgac gccgctgctc ttcgcgccgg cgacatcttt
     6961 ctggatgtgt tcgagatgga catggttcga aatgtcggaa tccagccgaa cgtaatccaa
     7021 accgagcaga ctgcaagcag cgccaagacg ctcgcggtca aagtcatatt gcgcctgaaa
     7081 aaggtaagcg tgcatctgcc agaatttgcc ttgcgcggac gcggcctctg ccgtttcagc
     7141 ggcaagttgc gctttgggat gcttggcaaa caaaggaaaa tggcgaaaca cgtaccgcag
     7201 attttcgccg aaccgcactt gcagccgggt gacaacctgg tgcaaggcgg cggtgtaagg
     7261 acattggtaa tcgccgtaca aaacaagcgt gaccgccgcg gaatcgggac cttgaacatg
     7321 gtcgtccccg gaaaccggca cagccagcgc gtgatgaaat aatccgtcgt tcatgggaaa
     7381 cccggcaaca ggatgaatca gtcatcctgt ctctattctt tacccgtttg cggtcgaact
     7441 gccatgcaaa tcctcagctt cccatccggt tcgattccga ctccagccaa catttcaaat
     7501 ctcctatttt tttgattctg ttgctggtgt tgatggcgga gcgatttcgt aacacgccat
     7561 ttcagcgccg ttgcggagca gaatgcggcc atcgccaatg gcgggtgcat tccagctttt
     7621 gccgtgcaac gcgggaaaac gcgcaatttc ggttagcttt tccggagtcg ctttgacgag
     7681 cgcgagatcg ccattggcgc aaaggacaat gagatggccg cttgcaagaa gcagttgacc
     7741 ataaccgtaa cggccatctt tccatttgcg ttcgccggtc tgcgcgtcaa cgcaggcgag
     7801 gatgtcttca tcgagcccat agatatatcc ctgccagaag acggcgctgg cgaatttggc
     7861 tttcaaactt ttagtgcgcc agagttcgtg cgtttcgtaa gcatcgccgg agcggtcaat
     7921 ttcataagcg ccgctcccgg tcatgtaagc cgcgctgagc atcagactgt tcgtgttgag
     7981 catgaggggt tgggtgattg gccgctcatg attgatgatg ggccaatgga tgcgccagcg
     8041 ttccgcgcca tcatcaaggc gcaaaccgat gacatccggc cgtccgcaaa caatcacctg
     8101 ccgctctcca ccgatcgttt caagcatcgg cgtggcgtaa cccatcggca aatcaagcgc
     8161 gtgccagaga atttttccgt cgcgtttgtc gtagcaaacg actgatttgc cgtgatacgc
     8221 atccggctga agaataattt tttcatccac gatcagcggg gacgcggcaa tgccgtaatc
     8281 gggcaatgcg gctttgttct ccgtcattat atttttggac cacacgacgg ttccgttcgt
     8341 ggcgttgacg caacataatt cgcccgttgc gccgagcgcg taatcttttc catcgtcata
     8401 agcgggagtg gaacgcgggc cttcgtcgct gtggtattcg gtgaatttcg cggtccatcc
     8461 gttggtccag agttcgcgtc cggtggcgag atcgtaggcg gcgagcactt cataatcgcg
     8521 gcgttgttcg agcgtgaagg cgcggtcatc agccagcgca aaagaggaat agccgccgcc
     8581 gcagggctga tgccaaagaa gtttcaggcc ggtatcgggc cagtttgtga ggattggccg
     8641 ctcgtcatat tcgccgtttt gatgcgggcc gcgaaagcca tgccaattgg cgtgaatggt
     8701 cgaatttatg gcaggcgcgg gcccaagttt gctttgttgc aggcggtttt gttcgactgc
     8761 ggcgaaatcg ggtttggttt tcttccatgt cacaaccggc gcgtaccaac cgcgccattc
     8821 gagttccaca acgcccaggt gcagcagaaa aagtatgacc cacgcggcgc aatccagcgc
     8881 cagaacgaac cagcagaacg ccgacaaaag ttttttgaaa attgtgcgcc gtccgcgccg
     8941 taatcgaagc aagagcgcgg gcagcagcaa caacgcgacg gtcagtccga tgataagctg
     9001 gattacaaaa tgtctgggca tcgttcgatt cggttttcag gtcatcgcaa ttttgctggc
     9061 gaaagggccg atgcgtcaac ctttctattt ttgcgcggtc caatcaaaac ttttcaacct
     9121 gccagatttc aggtaaacgt ccagcaacgt atgaaagaaa atgattcgaa tgcgggccga
     9181 ttcgaccggc ggatatttct aaaagcttcc ggcatggcgc tcggccttgc tggacttgct
     9241 tca
//
LOCUS       DASZMM010000531         5686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3258977, whole genome shotgun sequence.
ACCESSION   DASZMM010000531 DASZMM010000000
VERSION     DASZMM010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3258977"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2454)
                     /locus_tag="VG754_12795"
     CDS             complement(<1..2454)
                     /locus_tag="VG754_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWF20140.1"
                     /translation="MNDDLRADPDTLLEAIQRQEGQQKRGKLKIFLGMAAGVGKTFAM
                     LEAARRQQSLGVDVVIGYVETHGRKDTDALMVDLPVIPRKLTEHRSVTLSEMDVDAVL
                     ARKPKLALVDELAHTNAPESRHPKRYQDILELLDTGIDVYTTLNVQHVESRAQTVEEI
                     TGSTMHETVPDSVLDMAEIELIDISPEDLMRRLDEGKVYMPDRAAVAMVNFFREGNLT
                     ALREMALRLAAERVGQDVRDYMQVMQIQGPWKTGHRLLVAVSPSPLSAQMVRWTRRLA
                     DSLDAQWMAVHVESSKMLSEADQARLAKNLALARELGAEVIATADEDVVRGVLRVARQ
                     HNATQIVVGKPARNQLGRFLSGGGFLRRLVAESGDIDIHVVRAEKADVPVTKEIFQMP
                     SQVAWKQYLVSVGAVIGTTLLNELLNPILGYRAVGFDFLLMVVTLALFVGRGPILVAA
                     ALSAFSWDYFFIPPLFTFYVRQPEDVMMLGMYFVVALGMGELNARFRNQERAERQREE
                     RTTALYLLTRELADVVTLEQIVKVVVSHIEKLFKAESAVLLAEPGGKLSAQPSPGSTL
                     KLSEKELSVAMWVFQHNTPAGRFTENLPLSGALYLPLAAPGGTLGVIGVGLPPNRPLN
                     LEQNNLLEALVRQAGLVIDRQRLHNAAQQAQLAAESERLSKTLLDSISHEMRTPIAAI
                     TSAVSALTDPKTAAQAGSADVLLDEIKVAGARLNRLVGNLLDIARVESGHVKPKLDWC
                     DVGDLINVSIKSVERELAGHQLEISLHPAIPLVRMDFVLMEQALINLLLNAAFHTPAG
                     TPVQIRARVENDELALTVAD"
     gene            complement(2799..3398)
                     /gene="kdpC"
                     /locus_tag="VG754_12800"
     CDS             complement(2799..3398)
                     /gene="kdpC"
                     /locus_tag="VG754_12800"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HWF20141.1"
                     /translation="MKTITHDFFSSIMATLVLAVVCCGIYPLVVYGIAQAAFHDKANG
                     SLIMDAGGTLRGSALLGQQFTGDKYFDSRPSAAGNGYDGLNSGGSNLGPTSQKLNDAI
                     KDRIADYRKKNGLKDTDAVPADAVTASGSGLDPHISPANAALQSPRVAKVRGLSETKV
                     LELVAKYTEGPDLGILGEPGVNVLKLNLALDQLSTAGKK"
     gene            complement(3756..>5686)
                     /gene="kdpB"
                     /locus_tag="VG754_12805"
     CDS             complement(3756..>5686)
                     /gene="kdpB"
                     /locus_tag="VG754_12805"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HWF20142.1"
                     /translation="MIKNPVMFVTLVGAALTTVDIFLPTNERGVIIQIAVWLWFTVLF
                     ANFAEAVAEGRGKAQAKALRGTRTQTAANKITGKGKIETVDANCLRKGDMVVVRAGEV
                     IPGDGEVTEGAATVDESAITGESAPVVRESGGDRSAVTGGTRVLSDEITVRITADPGH
                     GFLDRMIALVEGAKRQKTPNEIALTIMLSALTLIFMFVIMALKPMGIYAAATFSVPVL
                     IALLVCLIPTTIGGLLSAIGIAGMDRLLQRNVLAMSGRAVEASGDVDVLLLDKTGTIT
                     LGNRQATEFVPASGVSEKDLADAAQLSSLADETPEGRSVVVLAKKFGIRAREIHELPN
                     AQFVKFSAQTRMSGVDLEGVQYRKGATDAICKFVNCHAPPEVAQTVERISRSGGTPLL
                     VGTKERILGTIYLKDIVKGGLPERFKRLRTMGIKTVMITGDNKLTAAAIALEAGVDDF
                     LAEAKPEDKLALIRKEQQGGRMIAMIGDGTNDAPALAQADVGVAMNTGTQAAKEAGNM
                     VDLDSNPTKLLEIIEIGKQLLITRGALTTFSIANDVAKYFAIIPAMLMATFPAISPLN
                     IMHLQSPGSAILSAVIFNALVIVALIPLALRGVKFQPIGALAILQRNLLIYGLGGVII
                     PFVGIKLIDVIITGLHLV"
BASE COUNT         1153 a         1755 c         1560 g         1218 t
ORIGIN      
        1 gtccgcgaca gtgagcgcca gttcgtcgtt ctcgactcgc gcgcggattt gcaccggcgt
       61 cccggcggga gtgtgaaacg ccgcgttcag caacagattg atcagcgcct gttccatgag
      121 aacaaaatcc atccgcacca gagggatggc gggatgcaaa gaaatttcca attgatggcc
      181 cgccagttcc cgctccaccg acttgatgga cacgttaatc aaatccccca cgtcgcacca
      241 atccaatttt ggcttcacgt ggccggattc cacgcgggcg atgtcgagca gatttcccac
      301 cagacggttg aggcgcgcgc ccgcgacttt gatttcgtcg agcaacacgt cggcagagcc
      361 ggcctgcgcc gcggtcttcg gatccgtcaa cgcgctcacc gcgctggtga tggcagcgat
      421 gggcgtgcgc atttcatgtg aaatggaatc aaggagtgtc ttgctcaaac gctccgattc
      481 ggcggcaagc tgcgcctgtt gcgccgcatt gtgcaggcgt tgccgatcaa tcaccagacc
      541 ggcctgccgc acgagagctt cgagcaaatt gttctgttcg agattgagcg gccggttcgg
      601 aggcagcccg acgccgatga cgcccaacgt cccgcccggc gcggccaacg gcaaatacaa
      661 cgcgcctgac aatggcagat tttccgtgaa ccggccggca ggcgtgttgt gctgaaagac
      721 ccacatcgcc acgctcaatt ccttttctga caacttcagc gtgctgccgg ggcttggctg
      781 cgcggacaat ttgccgccgg gttcggcaag caggaccgcc gattccgcct tgaacaattt
      841 ttcgatgtgc gacaccacca ccttcacgat ctgttccagc gtgaccacat ccgccagttc
      901 gcgcgtgagc aggtaaagcg cggtggtgcg ttcttcgcgc tggcgctcgg ccctttcctg
      961 gttgcgaaaa cgcgcgttca actcgcccat acccagcgca acgacgaaat acatcccaag
     1021 catcatcacg tcttccggtt ggcgcacata aaacgtgaac aagggcggaa tgaaaaaata
     1081 atcccaagaa aatgcgctca aggcagccgc gaccaaaatc ggcccgcgtc cgacaaacag
     1141 cgccagtgtc accaccatca acagaaaatc aaatcccaca gcgcgataac caaggatcgg
     1201 attcagcagc tcgttcaaca aggttgtgcc aatcacggct ccaacgctga cgaggtattg
     1261 tttccatgcc acttgcgatg gcatctggaa aatttccttg gtgaccggca cgtcggcttt
     1321 ttccgcgcgc acaacatgga tgtctatgtc gccgctttcc gcgacgagcc gccgcaagaa
     1381 accgcctcca ctgagaaagc gacccaactg atttcgggct ggtttgccga cgacaatttg
     1441 cgtggcatta tgctgccttg ccacccgcaa cacgccgcga accacatctt catccgcagt
     1501 cgcgatcact tccgcgccga gttcacgcgc cagcgccaga ttttttgcga gcctcgcctg
     1561 gtccgcttcg gaaagcattt tggaagattc cacatgcacg gccatccatt gcgcgtccaa
     1621 actgtccgca agccgccgcg tccagcggac catttgcgcc gagagcggac tcggactcac
     1681 cgcgaccagc aggcgatggc ccgttttcca aggcccctgg atttgcatca cctgcatgta
     1741 atcgcgcacg tcctggccga cacgttccgc agccagccgc aacgccattt cgcggagcgc
     1801 ggtcaagttc ccttcgcgga aaaaattcac catcgccact gccgcgcggt ccggcatata
     1861 gaccttgcct tcatcgagcc gccgcatcag gtcttccggc gaaatgtcaa tcaactcgat
     1921 ttccgccatg tcgagcacgc tgtcgggaac tgtttcgtgc atggtcgagc cggtgatctc
     1981 ctcgaccgtt tgagcgcggc tttcgacatg ctggacgttg agcgtggtat aaacgtcaat
     2041 gccggtatcg agcaattcga ggatgtcttg ataacgcttg ggatggcggg attcaggggc
     2101 gttggtatgg gcgagttcgt ccacaagggc gagcttcggc ttgcgcgcaa gcacggcgtc
     2161 cacgtccatc tcggaaagcg tcacgctgcg atgttccgtc agcttgcgcg ggatgaccgg
     2221 caggtcaacc atgagggcat cggtgtcctt gcgtccgtgg gtttcgacgt aaccgatgac
     2281 gacatccacg ccgagggatt gttgtctgcg cgcggcttcg agcatggcaa aggtcttgcc
     2341 cacgccggcg gccatgccga ggaaaatctt cagcttgccg cgcttttgct ggccctcctg
     2401 gcgttggatg gcctccagca acgtgtccgg atccgctcgc aggtcatcgt tcatttgatg
     2461 agttccgggt tccgagttcc cggttccaag ttatacgtgc atcctgaaat ctgcgacaac
     2521 tttccggccc cgaactgcaa gtgccgcagt ccgattggat tgcacacgca accgcaacaa
     2581 cattgtaatt cgcagtgacg cgctgcgtag cgcagagtta tactctgctg tatcgccgac
     2641 ttgcagtcgg ccggactgtc gaattgaaaa gagcgcccgg caatttgcag accggtggaa
     2701 attttgtcca tcttgcagag tacaactctg cgatacagca ggctgcaagc ctgcgctacg
     2761 aagcgcattg attttttgct ggtccctgct tctatacatc attttttccc cgcagtgctc
     2821 aattgatcca gtgccagatt cagtttcaat acgttcactc cgggttcacc caagatgccc
     2881 aaatcaggcc cctcggtata tttggcaacc aattccagaa cctttgtttc gctcaagccc
     2941 cgcactttgg ccacgcgagg cgattgcagc gcggcgttgg cgggactgat atgcgggtca
     3001 aggccgctgc cggacgcagt gacggcatcc gcaggcacag cgtccgtgtc cttcaaacca
     3061 tttttcttcc gataatcggc gatgcggtcc ttgatggcgt cgttgagttt ttgcgaagtc
     3121 gggccaagat tgctgccgcc cgaattcagg ccgtcgtagc cgttgcccgc cgccgaggga
     3181 cgcgagtcga aatatttgtc gccggtaaat tgctgtccca gcaacgccga accgcgcaac
     3241 gtgccgcctg cgtccataat caggctgccg tttgccttgt catggaaagc cgcctgcgca
     3301 atcccataaa caaccagtgg ataaatgccg cagcaaacaa ccgccaatac cagcgtcgcc
     3361 atgatggagc tgaagaaatc gtgagttatc gttttcatgg aatcacagtt gaatgaatga
     3421 ttgttggagc gatgggcact gcatcggatt gtcccaatgc attgcacggc ggagccgtgc
     3481 cagaaaatag ccgggggttg agcggagcga tacccccggt ttcacgacac gaaagcattg
     3541 caccctgaaa gggtgccacc aatccgcgcc tattgaatcc agtattttct gccacccctt
     3601 cggggtggat ttattcttgt tgcccgttcc gggggtatcg cttgcgctca acccccggct
     3661 attttcttgc aaccctccgg gttgcttttg tgagcatgcc cgacctctgg aggttcgcgt
     3721 taagctcttc gagaatgcaa ttttcatata agcagttaaa ccaaatgcaa ccccgtgatg
     3781 atcacatcaa tcagcttgat gccgacaaac gggatgatga cgccgcccag cccgtaaatc
     3841 agcaaattgc gttgcagaat tgccagcgcg ccgatgggtt ggaatttcac accccgcaac
     3901 gccagcggaa tcaacgccac gatgaccaat gcgttgaaaa tcaccgcgct caaaatcgcg
     3961 ctgccggggc tttgcaaatg catgatgttc agcggcgaaa tcgccgggaa cgtcgccatc
     4021 agcatcgccg gaatgatggc gaaatatttg gcaacgtcgt tcgcgatgct gaatgtcgtc
     4081 agcgcgccgc gcgtgatgag caactgcttt ccgatctcga tgatctcaag cagcttcgtc
     4141 gggttcgaat caaggtccac catgttgccg gcttccttgg cagcctgggt gccggtgttc
     4201 atcgccacgc caacgtccgc ctgcgccagg gccggcgcgt cattcgtgcc gtcgccgatc
     4261 atcgcgatca tccgtccgcc ctgctgttcc ttgcggatca acgccagctt gtcctcgggt
     4321 ttggcttccg cgaggaaatc gtccacgccc gcttccagcg caatcgccgc cgcggtcaat
     4381 ttgttgtcgc cggtgatcat caccgtcttg atgcccatgg tgcgcagccg cttgaaacgc
     4441 tcgggcaatc cgcctttgac gatgtccttg agataaatcg tgccgagaat gcgctccttg
     4501 gtcccgacca gcagcggcgt cccgcccgaa cgggaaatgc gctcaacggt ttgggcgact
     4561 tccggcggcg catggcaatt cacgaatttg caaatggcat ccgtcgcgcc cttgcggtat
     4621 tgcacgcctt ccaggtccac gccgctcatg cgggtctgcg cgctgaactt cacgaattgc
     4681 gcattgggca gttcatggat ttcccgcgcg cggattccaa attttttcgc aagcaccacc
     4741 acgctccggc cttcgggtgt ttcatcggcc agcgaagaaa gttgcgccgc gtccgccaaa
     4801 tctttttccg aaacgccgga cgccgggaca aattccgtcg cctgccggtt gccgagcgtg
     4861 atggtcccgg tcttgtccag cagcagcaca tccacgtcgc cagaagcctc gaccgcgcgt
     4921 ccgctcatcg ccagcacgtt gcgttgcagc aaccggtcca tgcccgcaat gccaatcgcg
     4981 ctgagcaatc cgccgatggt cgtcgggatg agacagacca gcagcgcgat caatactggc
     5041 acggagaatg tcgccgcagc gtaaattccc atcggcttca aggccatgat gacgaacatg
     5101 aaaatcaacg tcagcgcgga gagcatgatg gtgagcgcga tttcattggg tgtcttctga
     5161 cgtttggcgc cctcgaccag cgcgatcatc cggtccaaaa atccatggcc gggatctgcg
     5221 gtgatgcgta ccgtgatttc atccgacaac acacgtgtgc ctccggtgac agcgctgcgg
     5281 tcaccgccgc tttcacgcac aaccggggcg gattcgcctg tgatggccga ttcgtccacg
     5341 gtcgccgcgc cttcggtgac ttcgccgtcg cccggaatca cttcacccgc gcgaacgacc
     5401 accatgtcgc ctttgcgcag acagttggca tccacggttt caattttccc cttgccggtt
     5461 attttgttcg ccgcggtctg ggtgcgggtg ccgcgcaagg ccttggcttg cgctttgccg
     5521 cgcccttcag cgacagcttc ggcaaaattt gcgaacaaca cggtgaacca gagccagacc
     5581 gcgatttgaa tgatgacgcc ccgttcgttg gttggcaaaa aaatgtccac agtcgtcaac
     5641 gccgcgccta caagcgtcac gaacatcacc ggatttttga tcatgg
//
LOCUS       DASZMM010000532         6576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2703602, whole genome shotgun sequence.
ACCESSION   DASZMM010000532 DASZMM010000000
VERSION     DASZMM010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703602"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..218)
                     /locus_tag="VG754_12810"
     CDS             complement(18..218)
                     /locus_tag="VG754_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20143.1"
                     /translation="MKVIIQSIATRTYLKSLKQWTSELDEAKNFGYFERAAHFVRKQN
                     LREVQFAVPLPESDEVIVFPVV"
     gene            495..1247
                     /locus_tag="VG754_12815"
     CDS             495..1247
                     /locus_tag="VG754_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWF20144.1"
                     /translation="MSRQRLDQALVERGLCESREKAQRAIMAGQVLVNQQPARKSSEQ
                     VSSDDNIALTAPEKYVSRGGFKLEHALQHFQLDVTGQTAIDLGASTGGFTDCLLQHGA
                     AKVHAVDVGHGQLAWKLRQDPRVVVMEKTNARELTPAHFPAPFAPMDLAVIDCSFISL
                     SRLLPVAVALLRPFGKMVALIKPQFEAGKAEADKGRGVITDPAIHARVLAEVESFARA
                     QPRLKWRGVTESPLLGPAGNKEFLALLEKTGN"
     gene            1228..2106
                     /locus_tag="VG754_12820"
     CDS             1228..2106
                     /locus_tag="VG754_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWF20145.1"
                     /translation="MKKPAIKRVGLVGNSEKVSARAVVTEAARLITRAGRRVYCDVAT
                     AQLAGFKSGVFPDVCALTEAVDLLLVFGGDGTMLRVASEVAGSRTPVLGINIGGLGFL
                     TAVSSSEMPKALKQVWNGEFTLETRAMIDVTGRAQGRDIQACALNDFVISRGIVSRLI
                     TLEVKVDDESLTRYRCDGLIVSSPTGSTAYALSAGGAVVHPQSEVFQLTPICPHTLSN
                     RSVIVGLDSRIEVKVASPRPETILSADGQGICQIANGESVTIMRSRDSVCLMHLAGTS
                     FFETLRRKLHWSGANV"
     gene            2301..3311
                     /locus_tag="VG754_12825"
     CDS             2301..3311
                     /locus_tag="VG754_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWF20146.1"
                     /translation="MIRLKDIAQRAGVSVMTVSKVMRDAPDISAATKVRVKSLAQQMG
                     YVPDSNAQSLRTRTSRLMGVIIPSVTEPNFARVILAIEERAHELGFELILAHTFNQEG
                     REEACIQRLLARRVDGLFISPVYRLRPDAPVYQILQARGTPVVIIGHSAPFCRQFFNV
                     ESDDVQGSYAATQHLLQLGHRHIAFFGGPQAAPWAQERFEGYRRALRDANLDVEDRLV
                     FQAGSTIEDGAKTALQFVNESTKATAIQAVNDLVAIGCADTFLNQGIKIPDQLSVVGF
                     GNILTSEYFRVPMTTVRQPKFRLGAAAMDSMVKLLRRERPESKRLRAELIIRASTAAP
                     AA"
     gene            3378..3788
                     /locus_tag="VG754_12830"
     CDS             3378..3788
                     /locus_tag="VG754_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20147.1"
                     /translation="MKKVLLLIGLVAVLIAAGWSYQSLNAMRVNARTAKFNEDVDNLF
                     YALQQYKEKVGAWPVGSNGEIAKALMGNNSKNLMILVGRKQDLNSKGEFIDPWGTPLR
                     IYFAGEGIMVRSAGPNKRFDDSTVLNGDDYYRSN"
     gene            complement(3860..4987)
                     /locus_tag="VG754_12835"
     CDS             complement(3860..4987)
                     /locus_tag="VG754_12835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWF20148.1"
                     /translation="MNNKFLLLATAIVAKSNREHPADAVLRTELKNAKGLSRADSKSI
                     SDAVFAYYRWLGWLDRKTSIEQQLERAVELDKAFQENPQSISESELPRAVPEWVAGHV
                     KITPEWLRSLQMKPKLWLRARVGQGRGLAQKLGECWLADGALADAVRYEGPEDLFRTA
                     EFHAGEFELQDISSQIVGLICNPQPGETWWDACAGEGGKALHLCDLMRNKGLLWASDR
                     AEWRLKKLKLRAARAKLFNYRAALWDGGAKLPTKTKFDGVLVDAPCSGLGTWQRNPQA
                     RWTTTPDDVEELNVLQKSLLTNVATALKPGGRLIYSVCTLTEAETIGVTEAITQKFPD
                     LKPLMFSNPGDPDAQPAAHLNLRPQDVQGNGMFICGWKKTG"
     gene            complement(5037..5252)
                     /locus_tag="VG754_12840"
     CDS             complement(5037..5252)
                     /locus_tag="VG754_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002776225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF20149.1"
                     /translation="MKLNSYEMVIWWSQEDGAYVVDVPELSGCMAHGKTRQEAIGNAE
                     AAIKFWIKTAKEDGLDIPQPRGRLIFA"
     gene            complement(5249..5491)
                     /locus_tag="VG754_12845"
     CDS             complement(5249..5491)
                     /locus_tag="VG754_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWF20150.1"
                     /translation="MSVEKILEKLLRGESEANIRFDELCHLLQAKGFRMRISGSHHIF
                     TRAGVMERINIQRDGSKAKPYQVRQVRKILANYKLL"
     gene            complement(5519..6025)
                     /locus_tag="VG754_12850"
     CDS             complement(5519..6025)
                     /locus_tag="VG754_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HWF20151.1"
                     /translation="MNCPRCSGALEPVEYDGVVIQVCSNCKGEWLHAGELLKIVEHHD
                     EVFTPGEIDSLEPVNKEIFTAESEDHDELNCPACNAQMEHFNYGDTSGIILHKCLECG
                     GVWMDKDQLREVEEVVDGWKACLGKDVEKYGGVLDKIAVEEQKQLDQSVSISRFGFVN
                     AVLRHVCE"
     gene            complement(6061..>6576)
                     /gene="acnA"
                     /locus_tag="VG754_12855"
     CDS             complement(6061..>6576)
                     /gene="acnA"
                     /locus_tag="VG754_12855"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417109.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWF20152.1"
                     /translation="VRIKNLMVPGVEGGVTKIQGASEPVSIFDAAMNYAKTKAPLIVL
                     AGQEYGTGSSRDWAAKGTNLLGVKVVVAQSFERIHRSNLVGMGVLPLQFKEGTTAQTL
                     KLDGTETYDVVGLGADLKPQQDLALRITRANGQVENVPVRCRIDTPIEIDYYQHGGIL
                     PFVLRQLVAKA"
BASE COUNT         1409 a         1916 c         1722 g         1529 t
ORIGIN      
        1 cgactgctac tgcggaacta aaccaccggg aacacaatta cttcgtcgga ttctggcaag
       61 ggcaccgcga attggacctc gcgcaggttt tgtttgcgca cgaaatgcgc cgcccgctcg
      121 aaataaccga agtttttcgc ctcgtccagt tcgctcgtcc attgtttcaa cgatttcaga
      181 taggtcctgg tcgctatgct ttgaatgatc actttcatgg ccaaaggttt ggttttttgc
      241 cgatgcatat ttaacagcca acacccatca tcaaactcac ggattgcagg agccgaacag
      301 cctccaaact agaataacaa atgaactgca taatccgggc caactttatt ttcatgcgca
      361 aagtgctgca aatcagtctt tcgaataatt ctttcccacc gttcacgaca atttgcaaaa
      421 atttgctttg cagcttgcaa ccatttgcaa gtcggagttg tcgccgtgaa aacgaggcgg
      481 catactgccg atgaatgagc cggcagcgat tggaccaggc attggtggaa cgcggtttgt
      541 gcgaaagccg cgagaaggcc caacgcgcca tcatggccgg acaagtgctt gtcaaccagc
      601 aacccgcccg caaatcgagc gaacaggttt catccgacga taacattgcc ctgaccgcgc
      661 cggaaaaata cgtgagccgc ggcggattca aactggagca cgcgctccaa cactttcaat
      721 tggatgtgac cggccagacc gcgattgatt tgggcgcttc gaccggcgga tttaccgatt
      781 gcctgttgca gcatggcgcg gcaaaagtcc atgccgtgga cgtgggccac ggccagcttg
      841 cgtggaaatt acgccaggac ccgcgcgtgg tcgtgatgga aaagacgaac gcgcgcgaat
      901 tgactccagc gcattttccc gcgccattcg cgccgatgga tttggcggtg atcgattgtt
      961 cgttcatttc gctgagccgg cttcttccgg ttgcggttgc attgctccgg ccattcggta
     1021 aaatggtcgc cctgatcaag ccgcagttcg aggcagggaa ggccgaggcg gacaaaggca
     1081 ggggcgtcat caccgacccc gccattcacg cccgggtgct ggccgaagtg gagtcgtttg
     1141 cgcgcgcgca accgcggttg aaatggcgcg gcgtgacgga atcgccgctg ctcggcccgg
     1201 cgggaaataa ggaatttttg gcactgcttg aaaaaaccgg caattaaacg cgtcggactc
     1261 gtcggaaatt cagaaaaagt ttccgcgcgc gccgtcgtca ccgaagccgc ccggctcatc
     1321 acccgggcgg gacgccgcgt ttactgcgac gttgcgaccg cgcagctcgc cgggttcaaa
     1381 tcgggagttt ttccggatgt ctgcgcgctc accgaggcgg tggatttgct cctggttttt
     1441 ggcggggacg gcacgatgct gcgcgtcgcc agcgaagtgg ccggttcgcg aacgcccgtt
     1501 ctcggcatca acatcggcgg gcttggtttt ctcacggcgg tttcgtcgag cgaaatgccg
     1561 aaggcgttga agcaggtctg gaacggcgaa ttcacgctgg aaacccgcgc gatgatcgac
     1621 gtcacgggcc gcgctcaagg acgcgacatt caagcttgcg cgctgaacga tttcgtcatc
     1681 agccgcggca tcgtctcgcg attgatcacg ctcgaagtga aggtggatga tgagtcgctg
     1741 acgcgttatc gttgcgacgg cttgattgtc agttcgccca ctggttcgac tgcctacgcg
     1801 ctttcggcag gcggcgcggt tgtccatcct caatcggaag tgttccaact cacgccgatt
     1861 tgtccgcaca cgctttcgaa ccgctcggtg attgtcgggc ttgattcgcg catcgaggta
     1921 aaagtcgcaa gcccgcgccc ggaaacaatc ttgagcgcgg acggccaagg aatctgccaa
     1981 atcgccaacg gcgaaagcgt gaccatcatg cgcagccgcg attccgtgtg cctgatgcat
     2041 cttgcgggca cttcattctt cgagaccttg cggcgcaagc tgcattggag cggggcgaat
     2101 gtgtgagcga ggctgctgct gaatggttac atggttacat ggtcacatgg ttacatggtt
     2161 acaacgtcac agatttttgg ttgctcagta atatccgaag cggcgccaat gtaactttgt
     2221 aacgatgtaa ctatgtaacc ttttcgcccc gcaacacagg ctggacattc tccggtcaat
     2281 cctgccatct tttctccgac atgattcgcc tcaaagacat cgcccaacgc gccggagttt
     2341 cggtgatgac ggtgtcgaag gtgatgcgcg acgcgccgga catttcggcg gccaccaaag
     2401 tgcgggtcaa atcgctcgcc caacaaatgg gctatgtgcc ggattcgaac gcacaaagtt
     2461 tgcgcacgcg cacgagccgc ttgatgggcg tgatcatccc atccgtcacc gagccaaatt
     2521 tcgcgcgcgt tatcctggca atcgaggagc gcgcgcacga gctgggtttc gaactcatcc
     2581 ttgcccacac gttcaaccag gaaggacggg aggaagcctg catccaacga ttgctggcgc
     2641 gtcgcgtgga tggtttgttc atctcgccgg tctatcgttt gcgaccggac gcgccggttt
     2701 accagattct tcaggcgcgc gggacgccgg tggtgatcat tggccatagc gcgccgttct
     2761 gccggcaatt tttcaatgtc gaatccgacg atgtccaggg cagttacgcg gcgacgcagc
     2821 acctgcttca gcttggccat cggcacatcg cgttttttgg agggccgcaa gccgcgccgt
     2881 gggcgcagga acgatttgaa ggttatcgcc gcgctttgcg cgatgccaat ttggatgtgg
     2941 aagaccgcct ggtgttccag gccggaagca ccatcgagga cggcgccaaa accgcgttgc
     3001 aattcgtcaa tgaatccacc aaggcaactg ccatccaggc tgtgaacgat ttggtcgcca
     3061 tcggctgtgc ggacacgttc ctgaatcagg gcattaaaat ccccgaccag ctttcggtcg
     3121 ttggtttcgg caacattttg accagcgaat attttcgcgt cccgatgacc accgtgcgcc
     3181 agcccaaatt ccggctcggc gcggcggcga tggattccat ggtaaagctg cttcgccgcg
     3241 aacggcccga gtccaaacgc ctgcgcgcag aactcatcat ccgcgccagc accgccgcgc
     3301 ccgcggcgtg atccattggc aaagcacatt gcaatttgcg tgcgtttgtt ggcattcccc
     3361 ttgctaatag ccaacgcatg aagaaagtgc tgttactcat cggacttgta gccgtcctga
     3421 tcgctgcggg ttggtcatat caatcgctta acgccatgcg cgtcaatgcc cgcacggcga
     3481 agtttaacga ggacgtggac aacctctttt acgccttgca acaatacaag gaaaaggtcg
     3541 gggcgtggcc cgtcggcagc aacggggaaa tcgccaaggc cttgatgggc aataactcaa
     3601 agaacctcat gattttggtc ggtcgcaagc aagaccttaa ttccaagggc gaattcatcg
     3661 atccttgggg cacgccgttg cgcatctatt tcgccggtga aggcatcatg gtccgctccg
     3721 ccgggccgaa caaacggttc gatgacagca ctgtcctaaa tggagacgat tactatcgtt
     3781 caaattagac agtcttttgg cacgtagctg aaaacggaag ttttcagtag caattttcga
     3841 aggctgaaaa cgcaagtttt cagcccgttt ttttccaccc gcaaataaac atcccgttcc
     3901 cctgcacatc ctgcggcctg agattcaaat gagccgcggg ttgcgcgtcc gggtcgcctg
     3961 gattggaaaa catcagcggc ttcaaatctg gaaatttttg cgtgatggct tccgtcactc
     4021 caattgtctc cgcctccgtc aaggtgcaaa cggaataaat caatcgtcct ccgggtttca
     4081 atgccgttgc gacgttcgtg agcaagctct tttgcagtac gttcaattct tccacgtcgt
     4141 ccggcgtcgt cgtccaacgc gcctgcggat tgcgttgcca cgtgcccaag ccgctgcagg
     4201 gcgcatccac aaggacgccg tcgaattttg ttttcgtcgg cagtttcgcg ccgccgtccc
     4261 ataacgcggc gcggtaattg aaaagttttg cgcgcgcggc gcggagcttc agttttttca
     4321 aacgccattc cgcgcggtcg cttgcccaca gcaatccctt gttgcgcatc aagtcgcaaa
     4381 gatgcagcgc tttgccgcct tcacccgcgc aagcgtccca ccaggtttcg cctggttgcg
     4441 gattgcaaat caacccgacg atttgcgaac tgatgtcctg caattcaaat tctccggcgt
     4501 ggaattcagc ggtgcgaaac aaatcctccg ggccttcata ccgaaccgca tccgccaacg
     4561 cgccatcggc cagccagcac tcgcccaatt tttgcgccaa gcccctcccc tgccccacgc
     4621 gcgcgcgcaa ccaaagcttc ggcttcatct gtaaactcct gagccattcg ggtgtgattt
     4681 ttacgtgccc ggcaacccat tccggaacgg cgcgcgggag ttccgattca gaaatgcttt
     4741 gaggattttc ttgaaacgct ttatcgagtt caaccgcgcg ctcaagttgt tgctcaattg
     4801 aagtttttcg gtccaaccag cccagccagc gataatacgc gaacaccgcg tcgctgatgc
     4861 tcttgctgtc cgcgcgtgaa agccccttgg cattcttcaa ctcggtgcgc aacaccgcgt
     4921 ctgcgggatg ttcacggttg gattttgcga caatggcggt cgcgaggagt aaaaatttat
     4981 tgttcatttt ggccggctca ggagattgta tgccgacaaa ttagaacaat tgaaaatcag
     5041 gcaaaaatca gccgcccgcg aggctgagga atatctaatc catcttcctt ggcggtcttg
     5101 atccaaaatt tgatggccgc ctcggcgttg ccgatggctt cctgccgtgt tttcccatgg
     5161 gccatgcagc cggaaagctc gggcacatcg accacataag ccccatcctc ttgactccac
     5221 caaatcacca tctcgtaact attgagtttc ataacaattt gtaatttgcc aaaattttgc
     5281 gcacctgtct aacctgatat ggtttggcct ttgaaccatc acgttggatg ttaatccgct
     5341 ccattactcc agcgcgcgta aatatgtggt gacttccaga tattcgcatc cgaaaacctt
     5401 ttgcttgcaa caaatgacaa agctcatcaa agcgaatgtt cgcttctgat tctcctcgca
     5461 aaagtttctc cagaatcttt tcaacgctca ttgtgtcgtt ttggagtttc agaacaattc
     5521 actcgcaaac gtgccgcaaa acggcattta cgaagccaaa tctcgaaatc gaaacactct
     5581 ggtccagttg cttttgctct tcgactgcta tcttgtcgag gacgcctccg tatttttcca
     5641 cgtcctttcc caggcaggcc ttccatccgt caacgacctc ctccacttca cgcaattggt
     5701 ccttgtccat ccaaacaccg ccgcattcaa ggcacttgtg caggatgatt ccgcttgtgt
     5761 cgccataatt gaaatgttcc atctgcgcat tgcacgcggg acaattcaat tcgtcgtggt
     5821 cttccgattc agcggtgaaa atctccttgt tgaccggctc aagcgaatcg atttctccgg
     5881 gcgtaaaaac ttcatcgtga tgctccacga ttttcagcaa ctctcccgcg tgcaaccatt
     5941 cccctttgca attggagcag acttgaatca caactccatc atattcgacc ggttccaacg
     6001 caccgctgca ccttggacaa ttcataattt taaattcgac ttctgattga ctgtcgtgac
     6061 ttacgccttc gcgaccaact gccgcaacac aaagggcaaa atgccgccgt gctgataata
     6121 gtcaatttca atcggcgtgt cgatgcggca gcgcacggga acattttcca cctggccgtt
     6181 cgcgcgcgtg atgcgcaacg cgaggtcttg ctgcggcttc agatccgcgc caaggcccac
     6241 aacatcatac gtctcggtgc catccagctt gagcgtctgc gcggtcgtgc cttccttgaa
     6301 ttgcaatggc aacacgccca tgccgaccag attggaacga tgaatgcgct cgaagctctg
     6361 tgccacgacc actttcacgc ccagaagatt tgttcctttc gccgcccagt cgcgcgaact
     6421 gccggtgccg tattcctgtc cggccagcac aatcaatggc gctttcgttt tcgcataatt
     6481 catcgccgca tcgaaaatgc tgacgggctc actcgcgccc tgaattttcg tcacgccgcc
     6541 ttcgacgccc ggaaccatca aatttttgat gcgcac
//
LOCUS       DASZMM010000533         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5459045, whole genome shotgun sequence.
ACCESSION   DASZMM010000533 DASZMM010000000
VERSION     DASZMM010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5459045"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VG754_12860"
     CDS             <1..1782
                     /locus_tag="VG754_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWF20153.1"
                     /translation="FWSGTESLTNRVVRVIYRDHENNLWFGCSPGLVLDSSSKKFLEK
                     DEIRCLAEDQHGTLWVGTSEGLYHRDKNGAFRPFKMRRELAKNAVLALYADQEGNLWA
                     SIANCGLACIQNGRLSICTMKQGLWNDEIFEILEDDHGWLWMSCPKGVFRVNKTQLAD
                     LNQGLAKTITCIAYGKTDGMESIQCSGVSKPAAWRSRDGRLWFATAKGLAVTNPNEDL
                     VLNEKPPTVRIEEVLIDKRLINFSEDAPLQIKSGRGELEIHYTALSFQMPEKNHFRYR
                     LGGVDPEWVDAGNRRVAYYNNLRPGSYVFQVRACNNDGVWNEKPAEFEMTLLPHFWQL
                     KWFSGFAALAIAGMIGVTVRYATRKKLERKLEGLEQQNAIEKERTRIAQDMHDDLGAR
                     LTEILLLNELARKNKQSSDNLENYLGKQSHVVRDVADSLDTIVWAVNPTKDTLDSLAN
                     YLYEQVERLLTMKSIRCRFDVPDQLPSCFISSEVRHNVFLTVRESLNNVIKHSGATEV
                     WFRLRHSSASLSIVIEDNGKGISPAERSACGNGLQNMERRMKNLGGSFRLSSHSKRGT
                     QIRLEVPLRPVPEGTRRFPGINSIEQI"
     gene            1803..2435
                     /locus_tag="VG754_12865"
     CDS             1803..2435
                     /locus_tag="VG754_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF20154.1"
                     /translation="MPANAKVAIVEDDTGVRESLAVLINGASGFRCTGSYANAEAALK
                     QLPENWSDIVLMDINLPQMSGIDCVAKLKAMRPGLRVLMLTVHVDSELIFKSLKAGAD
                     GYLIKQTSPAEILDALSDVFRGGAPMTNTIARKVVLFFQQQSAADDETACLTKRELEI
                     LTHLAKGFQYKEIADLLGITLLTVRTHIHHIYEKLHVRSRTEAVVKFLGK"
     gene            2674..3432
                     /locus_tag="VG754_12870"
     CDS             2674..3432
                     /locus_tag="VG754_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HWF20155.1"
                     /translation="MKKILGYSAALLSVSLVAPSAMAQRPLGVDVSSYQGGSINWGSV
                     YSDGVRFAFAKCTEGYNYFVDPDYGGNMSRGKTALLQMGAYHFAHPYANYPSQEAGYF
                     WSHANGYIKADGKSLMPMIDFEEFSGHVGTSTYTQWFNDWSADIIADGHAAHVTLRPV
                     IYCSAGTGACDLNTSIALGGWIANYNGENLYTGNPWSCCTGCNAWAPGTQNNWTYWQV
                     SSTGRITGISGNVDLDTYPDNLTDLKAWQGVTSY"
BASE COUNT          849 a          945 c          920 g          809 t
ORIGIN      
        1 ttctggtccg gaaccgaatc gctcaccaat cgagtcgtcc gggtgattta ccgcgatcac
       61 gagaacaatc tttggttcgg gtgcagtccg gggctggtgc tcgacagttc cagcaagaag
      121 ttcttggaaa aggatgagat ccggtgcctt gccgaagatc agcacggcac tctctgggtc
      181 ggaacgagcg agggattgta tcatcgtgat aaaaatggcg cgttcaggcc gtttaaaatg
      241 cgccgcgaac ttgccaagaa tgccgtgctt gctctttatg cggaccagga aggaaacctt
      301 tgggcaagca ttgcgaattg cggacttgct tgcattcaga acgggcgctt gagcatttgc
      361 acaatgaaac aaggtctgtg gaatgatgaa atttttgaaa tcttggaaga cgaccatggc
      421 tggctttgga tgagctgtcc caagggcgtt ttccgcgtca acaaaacgca attggctgac
      481 ctgaatcaag gattggccaa aacgatcacc tgcatcgcct acggcaagac ggatggaatg
      541 gaaagcattc aatgcagcgg agtttccaaa ccagccgcct ggcgcagccg cgatggacgg
      601 ctctggtttg cgacagccaa aggcctggcg gtgacgaatc ccaatgagga cttggtttta
      661 aatgagaagc cgccgactgt ccgcatagag gaagttctta tagacaaacg gctcatcaat
      721 ttcagcgagg atgctccttt gcaaatcaaa agcggacgtg gagaactgga aattcattat
      781 accgcgctga gttttcagat gcccgagaaa aaccattttc gctatcgcct cggcggggtg
      841 gatccagaat gggtggatgc gggaaaccgg cgtgtggcgt actacaacaa tttgcggccg
      901 ggaagttatg tgttccaagt cagggcctgc aacaacgacg gcgtttggaa cgaaaaaccg
      961 gcggaatttg agatgacgtt gctgccgcat ttctggcagt tgaaatggtt cagcgggttt
     1021 gcagcattgg caattgccgg gatgattggc gtcactgttc gttatgcgac acggaaaaaa
     1081 cttgaacgca aactggaggg cttggaacag caaaatgcga tcgagaaaga acgcacacgc
     1141 atcgcccagg acatgcacga tgaccttgga gcgcgcttga cggaaattct gcttttgaac
     1201 gaactcgcgc gaaaaaacaa gcaaagctcc gacaacctcg aaaattatct tggcaaacaa
     1261 tcgcacgttg tccgcgacgt tgccgacagc cttgatacga ttgtctgggc ggtcaacccc
     1321 acgaaggaca ctctcgacag ccttgccaac tatctttatg aacaggtcga acgtctcctg
     1381 acaatgaaat ccatccgatg ccggtttgac gttccggatc aattgccctc ctgcttcatt
     1441 tcatctgaag tgcggcacaa tgttttcctg acggtcaggg aaagtctgaa caatgtgatc
     1501 aaacattccg gcgcgaccga ggtctggttc cggttgcgcc acagttcagc gtctctttcc
     1561 atcgtgattg aagacaatgg aaagggcatt tcaccggcag agcgttccgc gtgcggaaat
     1621 gggctgcaga acatggagcg gcgaatgaaa aaccttgggg gttctttccg tttgtccagc
     1681 cattcaaaaa gaggcacgca aatccgtctt gaagttccgc ttcgccctgt ccccgaagga
     1741 acaaggcgtt tcccgggaat aaattccatc gagcaaatct gaaactctga cccaaaccta
     1801 atatgcctgc caacgccaaa gtcgcaattg tcgaggacga tacaggtgtc cgggaaagtc
     1861 tcgccgtcct gatcaacggc gcctccggct ttcggtgcac tggctcttac gcaaacgcgg
     1921 aagccgcgtt gaaacaactt cccgaaaact ggtcggacat cgtcctgatg gacatcaatc
     1981 ttccccaaat gtcgggcatt gattgcgtgg ccaaattgaa ggccatgcgg cccggcttgc
     2041 gcgttctcat gctgacggtg catgtggaca gcgaactgat tttcaagtcc ctcaaggctg
     2101 gcgcggatgg ttacctcatc aaacagacct cgcctgcgga aattctcgac gcattgtccg
     2161 acgtttttcg tggcggcgcg cccatgacca ataccattgc ccgcaaagtc gttctgtttt
     2221 ttcaacaaca gagcgctgcg gatgatgaaa ccgcctgcct gacgaaacgc gagcttgaaa
     2281 tcctcaccca tctcgccaag ggctttcaat ataaagaaat cgctgatttg cttggcatca
     2341 ccctcctcac cgtccgcact catatccatc acatctacga aaagttgcac gtccgctccc
     2401 gcactgaagc tgttgtgaaa ttcctcggaa aatagttcgc gagcgttccg tctttttaga
     2461 attatttcta gtatgtacgt tgtatttttc acgtttctgc ggctgttttt ttgcaagaaa
     2521 atgatagtaa atgccgatgg cccgcatttt tgaagtttag tatctttcgt gatgtcagcc
     2581 atgagtgcgt tgtcctcctt cgtagatggc gcatgtcttc cggttcatgg caaacaaagt
     2641 caacaaacaa accacaaaag aaagacccag ataatgaaga aaatactcgg atactcggcc
     2701 gctcttctat cagtctcatt ggtcgcccct tccgcgatgg cgcaacgccc tttgggcgtt
     2761 gatgtctcct cctatcaagg cggctccatc aattggggga gcgtttactc ggacggcgta
     2821 aggttcgcct tcgccaagtg cactgaagga tataattatt tcgtggaccc cgattacggc
     2881 gggaacatgt cccgcggcaa gaccgccttg ttgcagatgg gcgcctatca tttcgcccat
     2941 ccctacgcca actacccatc ccaggaagcc ggttatttct ggagccatgc caacggttat
     3001 atcaaggctg acggcaagag cctcatgccg atgattgact tcgaggaatt ctccggacac
     3061 gtcggcacca gcacctacac ccaatggttc aatgattggt ccgctgacat catcgcggac
     3121 ggtcatgcgg cccatgtcac cctccgccct gtcatctatt gcagcgctgg caccggtgcc
     3181 tgcgatctta acaccagcat cgcgctcggc ggttggatcg ccaactacaa tggtgaaaac
     3241 ctctacaccg gcaatccttg gagctgctgc accggctgca atgcctgggc tccgggcact
     3301 cagaacaact ggacctactg gcaggtcagt tcgactggcc gaatcaccgg catctccggc
     3361 aatgtggact tggacaccta cccggacaac ctgaccgact tgaaggcctg gcagggcgtc
     3421 acctcctact aagctgcgcc gcttacgcgg gcaccggggg ttaagctccg tcccgtgtgg
     3481 atacagaccc gcgcaaattc ccagatattt gcgcgggttt ttt
//
LOCUS       DASZMM010000534         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4368900, whole genome shotgun sequence.
ACCESSION   DASZMM010000534 DASZMM010000000
VERSION     DASZMM010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4368900"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VG754_12875"
     CDS             complement(<1..901)
                     /locus_tag="VG754_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWF20156.1"
                     /translation="MPAASRRYSRLKICAALLLFSCFSAFAETNEIDSALSTTQFRGE
                     SSCSSSGCHGGAGEKHDQCIRWSKFDVHTRSYATLTLARSARIADVLKIGDPVQSARC
                     TVCHAPFQTIATNDALRKSLDPTAGVSCETCHGPAEKWLLSHTREDYTHQDRVNAGMR
                     DLNNIYVRANTCVACHQNVDADILQAGHPELIFELDGQAVAEPKHWREKPGWSGSQTW
                     FVGQAVALREMSWQLTPEKAPSQADLEQEQGLLWVVESVDWNSNVPQLMAAPSLDLKW
                     DKSYQQRVQSWADQMANSATYFPC"
     gene            1144..>1873
                     /locus_tag="VG754_12880"
     CDS             1144..>1873
                     /locus_tag="VG754_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWF20157.1"
                     /translation="MALGGNTALVLGYILLAIILIEGCLVIYLTLRRIYFQREQQRLT
                     RERLRLLVKAAALQCREAEQGKLVWNGYRKFRVAKKIQECHGVCSFYLKPHDGKSLPP
                     YKPGQYITFQLNLPGQGKPLIRCYSLSDSPHRGDYYRVTIKKEPAPSDKPGVPHGLAS
                     TYFTEVVKEGDILDVKAPAGNFCLDLAKETPVVLVGGGVGVTPMLSMAHAIAASNTRR
                     EVWFFFGARNSKEHIHKDEMLKLAA"
BASE COUNT          425 a          546 c          503 g          399 t
ORIGIN      
        1 ggcaaggaaa ataggtggct gaattcgcca tctggtcagc ccacgattgc acccgctgct
       61 gataactttt gtcccacttt aaatccaacg atggtgccgc cataagttgc gggacattcg
      121 aattccaatc aacactttcc accacccaaa gcaacccttg ctcctgctca aggtcagctt
      181 gggagggcgc tttttcaggg gtcaattgcc agctcatctc acgcagagca acggcctgac
      241 cgacgaacca cgtttgcgat ccggaccagc ccggtttctc gcgccaatgt ttcggctcgg
      301 cgactgcctg gccgtccaat tcaaaaatca attctggatg gccggcttgg agaatgtccg
      361 cgtcaacatt ttgatggcag gcgacacagg tgttcgcgcg cacgtaaata ttgttcaaat
      421 cgcgcatccc ggcattcacg cggtcttgat gggtgtaatc ctcgcgcgtg tgggaaagca
      481 gccatttttc ggcaggccca tgacaggttt cgcaggaaac tcccgcagtg gggtcgagac
      541 tttttcggag agcatcattg gtcgcaatcg tttgaaatgg cgcatggcaa accgtgcaac
      601 gcgcgctttg caccgggtcg ccaatcttca aaacatccgc gatgcgcgcc gagcgggcca
      661 gggttagggt cgcgtaggaa cgggtatgaa catcaaattt cgaccagcgg atgcattggt
      721 catgtttctc gcccgcgccg ccatggcatc cggaggaact gcacgatgat tcaccacgaa
      781 attgcgttgt ggaaagggct gaatctattt cgttggtctc ggcgaaggcg gaaaaacaac
      841 tgaacagcag cagcgcagcg cagattttta atctactgta tcgccgactt gcagccggca
      901 tgccggagta aacgaagtga attgtcggat atttcaagcg tatcgtgaaa tcacgggctc
      961 cccagattga aaatccgcga aacagcagag tgcaactctg cgctaccaga tgggcggtca
     1021 tagcatgatt cccaagcacg gccaattctt cccccggctt aaactaaagc gtttactccg
     1081 cacgtgacgt ttgttagata agctgagagc agaatgcaac caaatatcgt ccataaactg
     1141 cagatggctt tggggggcaa caccgcgctg gtccttggtt acattcttct ggcaatcatc
     1201 ctgatcgagg gttgtctcgt gatttacctc acgctgcggc ggatttattt ccagcgcgag
     1261 cagcagcgcc ttacccgcga acgcctccgt ctcctcgtca aggccgccgc gctccaatgc
     1321 cgcgaagccg aacagggcaa gctcgtctgg aacggttacc gcaaattccg cgtcgcgaaa
     1381 aaaatccagg aatgccacgg cgtgtgctcc ttttacctga agccccacga cggcaagtcg
     1441 ctgccgccct acaagcccgg ccaatacatc acgttccagt tgaatcttcc cggccagggc
     1501 aagccgctca tccgctgcta ttccctttcc gacagcccgc atcgcggcga ttactatcgc
     1561 gtcaccatca aaaaggagcc cgcgccatcc gacaagccgg gcgttccgca tggactcgcc
     1621 tcgacttatt tcaccgaagt tgtgaaggaa ggcgacatcc tggacgtgaa agcgccggcg
     1681 ggaaatttct gcctcgatct ggccaaggaa acgcccgtgg tgctcgtcgg cggcggcgtt
     1741 ggcgtgacgc ccatgttgag catggcgcac gcgattgccg ccagcaacac acgccgcgaa
     1801 gtctggtttt tcttcggtgc gcgcaattca aaagagcaca tccacaaaga cgagatgttg
     1861 aagcttgccg ccg
//
LOCUS       DASZMM010000535         6026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3259654, whole genome shotgun sequence.
ACCESSION   DASZMM010000535 DASZMM010000000
VERSION     DASZMM010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3259654"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VG754_12885"
     CDS             complement(<1..515)
                     /locus_tag="VG754_12885"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HWF20158.1"
                     /translation="MHYVILRDDDTNALTPVDCLELLYRPFLDRNLPVNLATIPEVSL
                     EAISPDGHPEAFLFGNTANSKTRTKPLAGNDELVSYLRKNSGYRIVQHGLHHDCFEFD
                     RDDRAEVRRRLQRGTDLLVTAGFSAPKTFVAPHDRFTRASLDETAKRFEVISSGWYEK
                     DRLPVAWWPRYL"
     gene            complement(487..1752)
                     /locus_tag="VG754_12890"
     CDS             complement(487..1752)
                     /locus_tag="VG754_12890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF20159.1"
                     /translation="MRVAHLLRKYNPAEWGGTETAIHRLFDGLRQHRVDSVVYAPKLE
                     RGPNVADPLRAAGCEVKRFKAFVPIWGMPEAEKRELVSVGGNLMSLDLIGALLRQKEI
                     SMVHSHALGRIGAIGWRAARWHKAPFVVTIHGGLLDLPEKLKANIQQPIKGFDWGKIF
                     GLLLHSRRLLDGADAIVTCNPREAALLQQKFPRKRIQVQAHGVVLELYQKDSRETALK
                     IFPALRGRDILLCVGRIDTVKNQIWLLEQMPGLLQKHPNAMLVLAGACTEEKYGKRIE
                     RRIAELGLAERVLLTGGLPPGDSRLIGLFQQAALLLLPSMSETFGLVLLEAWAAGTAV
                     ASSRTSGAKALIKEGENGWLFDLERPEEFHRAVQQCLTDASLRKRFAANGSELVATDY
                     NTFALAGRMKRLYEDLIEEKHALRDSARR"
     gene            complement(1756..3225)
                     /locus_tag="VG754_12895"
     CDS             complement(1756..3225)
                     /locus_tag="VG754_12895"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWF20160.1"
                     /translation="MSEYKTLFGIAVIPSAMLGGILLATFSRRLRDLFFFLFTLLCIM
                     TYRLDVNFVSREWYRGSTRGFEVSTLDILSVAVLFSSLLRPAEGRSRWYWPPGLGLML
                     VFFLYACFSVAISDPMLFGMFELSKMLRGFVVFFAAALYVRSERELKILVLALACVAC
                     WQGCVTLQQRYIQGFFRVEGSIGNPNSLSMYLCMIAPLLVAAFNADFPRWLKCICAVG
                     MMSAMLAVLLTISRAGFATLLVVMLGSTLACVSFKLSFRKILIGGLCFAMAVGMVAKA
                     WHTIQSRYDESSLSQEYKGHTQNRGYYITIAEAIAQDRFFGVGLNNWSYWVSNQYGPM
                     LGWHFVPYIDTENYPSDVVPPGRDIDAAQAAPAHNLGALTLGELGIPGLLIFTLLWVR
                     WFQMGASFLWWRVSDPTLRLGAGIFFCTWGIWLQSLTEWVYRQTAIFFTFNILMGTLA
                     SLYVMKRDRKRMEMEQKEQEDFAEEEEFDEMEIPAHSES"
     gene            complement(3256..4422)
                     /locus_tag="VG754_12900"
     CDS             complement(3256..4422)
                     /locus_tag="VG754_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF20161.1"
                     /translation="MILDPISWGASAWDDGFPGGEEARQMDVSIGIIAWNEEKSIGRA
                     LESLFRQTLFGKLAQRGLRCEIVCVANGCTDGTSDVVTKIFADQSHRHPCREIFSCRH
                     LDLKERGKINAWNCFVHQLSAPDARFLFLMDADIVINETEALWNMVAALEGNPHAVIS
                     TDKPCKHIAARRRKSFAERLSLRAGQMTRAGQAQLCAQLYCIRAETARRIHLPKDLPA
                     CEDGFIKAIVCTDFLTRDVNPLRIMTAPGASHTFEAYTTLRGVLRNQKRQMIGQAAVH
                     VLVDDYLKTLAVSERKNLAGTLREKDRKDPLWLKQLIAEHVRQTRFFWRLVPGLVGFR
                     FHRLARLKGFGKITCLPAAMAGFVLSMLSSFLAYGFLKKGGVSYWPHAKSPGQK"
     gene            complement(4590..5030)
                     /locus_tag="VG754_12905"
     CDS             complement(4590..5030)
                     /locus_tag="VG754_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWF20162.1"
                     /translation="MKISSSAFKENDTIPKKFTKDDANVSPPLHIDDIPSRAKSLALV
                     VEDPDAPSGTFHHWVLFNMSPRTADIHENSVPVMATQGRNDFGELEYGGPKPPSGEHH
                     YFFNVYALDTVLSLPRGTDSKALETEMKGHVVDQASLMGRYAHP"
     gene            complement(5027..5170)
                     /locus_tag="VG754_12910"
     CDS             complement(5027..5170)
                     /locus_tag="VG754_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20163.1"
                     /translation="MPTVSLDVALAHFAWVKKAELPLDGGFLKGEFNVTDFESGKIKR
                     RIA"
BASE COUNT         1488 a         1781 c         1481 g         1276 t
ORIGIN      
        1 agataacgcg gccaccatgc cactggcagc cggtcctttt cataccaacc ggatgaaatg
       61 acctcaaaac gtttcgcggt ttcgtccaag ctggcacgcg tgaaccgatc atgcggcgcg
      121 acaaacgttt tgggagcgga aaatcccgcc gtcacgagca aatcagtccc acgctgcagc
      181 cgcctgcgaa cttccgcccg atcatcgcgg tcaaattcaa aacaatcgtg atgcaaccca
      241 tgttgcacaa ttcgataacc cgaatttttc cgcaaataac tcaccaactc gtcattccct
      301 gcgagaggtt tcgttcgagt ctttgaattg gccgtgttgc caaataagaa tgcttccggg
      361 tggccatcgg gagaaattgc ttcgagcgac acttcaggaa tcgttgccag gtttaccggg
      421 agatttcggt cgaggaacgg acgataaagc agttccaaac aatccaccgg cgtcagggcg
      481 tttgtgtcat cgtcgcgcag aatcacgtaa tgcatgtttc tcctcgatta aatcctcata
      541 gaggcgtttc atccgtcccg caagggcgaa ggtgttgtaa tcggtcgcca ctagttcgct
      601 cccgttcgcc gcaaaccgtt ttctcaatga cgcatccgtc aggcattgct gaactgcgcg
      661 gtgaaattct tccggacgct ccaaatcaaa caaccagccg ttttcacctt ctttaatgag
      721 cgccttcgct ccagaggtgc ggctcgatgc cactgccgtg ccagccgccc aggcttccaa
      781 cagcaccaaa ccgaatgtct cggacattga cggcaaaagc aaaagcgccg cttgttgaaa
      841 gagtcctatc aatctcgaat cgccgggcgg caatcctccc gtcaacaaca cgcgctcagc
      901 caggccaagc tcggcaatcc ggcgttcaat gcgcttgcca tatttctcct cagtgcatgc
      961 gccagcgaga acaagcattg cattgggatg tttttgcaaa agcccgggca tctgttctag
     1021 aagccatatc tggtttttga ccgtgtcaat cctcccgacg cataacaaaa tatcgcggcc
     1081 tctcaatgcg gggaaaattt tcaaggcggt ttcacgggaa tccttttgat aaagctcgag
     1141 caccacgcca tgcgcttgca cttggatccg tttgcgcgga aatttttgct gcaacagcgc
     1201 ggcttcacga ggattgcacg tcacgattgc gtccgcgcca tcaagcagcc gacgcgaatg
     1261 cagcagcaac ccaaaaattt tgccccaatc aaatcctttg atcggttgct ggatgttcgc
     1321 cttcaacttt tccggcaaat cgagcaaccc accatggatg gtcacgacaa acggcgcctt
     1381 atgccatcgc gccgcccgcc agccaatcgc cccgatgcgt ccgagcgcgt gcgaatgtac
     1441 catgctgatt tccttttgcc ttaataatgc gcctatcaaa tcgagcgaca tcaaatttcc
     1501 tcccacggaa accagctcgc gtttttccgc ttccggcatt ccccaaatcg ggacgaaagc
     1561 tttgaatcgt ttcacttcgc atcctgccgc gcgcaacggg tccgccacgt ttggcccgcg
     1621 ttccagcttc ggagcgtaaa ccaccgaatc gacacggtgt tgccgcaacc cgtcgaacaa
     1681 tcgatgtatc gccgtctccg tcccgcccca ttcggcggga ttgtattttc tgagcagatg
     1741 ggcgacgcgc atggttcaac tttcgctgtg tgccggaatt tccatttcgt cgaattcctc
     1801 ttcttcggca aaatcctcct gctctttctg ttccatttcc atgcgcttgc ggtcgcgctt
     1861 catgacataa aggctggcca gcgtgcccat gaggatgttg aaggtgaaaa aaattgcggt
     1921 ctggcggtaa acccattccg tcaggctctg aagccaaatg ccccatgtgc aaaagaagat
     1981 gcccgcgcca aggcgcaacg tcgggtccga aacgcgccac cacaaaaaac tcgcgcccat
     2041 ctggaaccaa cggacccaga gcagcgtaaa aatcaacaga cccggaatgc ccagttcgcc
     2101 caaggtcaac gcgcccaagt tatgcgctgg cgcggcctgc gcggcatcga tgtccctgcc
     2161 cggaggaaca acatcgcttg gataattttc agtgtcgatg tacggcacaa aatgccagcc
     2221 caacatcggg ccatattgat tgctgaccca ataagaccaa ttgttcagcc caacgccgaa
     2281 aaaacggtcc tgggcgatgg cctccgcaat cgtgatgtaa tagccgcgat tttgtgtgtg
     2341 gcccttgtat tcctgcgaga ggctgctttc gtcgtaacgg gattggatcg tatgccacgc
     2401 tttcgcaacc attcccaccg ccattgcgaa gcacaatcct ccgatcagga tttttcggaa
     2461 actgagcttg aacgatacgc aggcgagcgt ggaacccagc attaccacga gcaacgtcgc
     2521 gaaaccggcg cgcgaaatgg tgagcagaac ggcgagcatc gcggacatca tgcccactgc
     2581 gcaaatgcat ttaagccagc gtgggaaatc cgcgttgaac gccgccacaa gcaacggcgc
     2641 gatcatgcac aggtacatcg aaaggctgtt cgggttgccg atggaccctt ccacgcgaaa
     2701 aaaaccttga atataacgct gttggagcgt gacacatccc tgccaacatg ccacgcaagc
     2761 cagcgcaagc accaaaatct tcaattccct ttcagaacgc acgtacagcg cggcggcgaa
     2821 aaaaaccacg aaaccgcgca gcatcttcga cagctcgaac atcccgaaaa gcatcgggtc
     2881 ggaaatcgcc acggaaaagc aggcataaag aaaaaacacg agcatcaatc caagacccgg
     2941 aggccagtac caacgtgaac gtccttcagc aggccgcagc agcgaactga ataaaaccgc
     3001 caccgacagt atgtccagcg tcgagacctc aaaaccccgc gtgctgccgc gataccattc
     3061 gcggctgacg aaattcacat ccagccggta agtcatgatg cacagcagcg tgaaaagaaa
     3121 aaagaacagg tcgcgcaagc gccgcgaaaa agtggcaagc aggattccgc ccagcatcgc
     3181 tgagggaatc accgcaattc cgaacaatgt cttgtattcc gacatgaatt tcgcgatgcg
     3241 gcaatccagc agccgctatt tctgcccggg cgatttcgca tggggccagt aggacacgcc
     3301 gcccttcttc aagaatccgt aagcgagaaa ggaactgagc atcgaaagga caaacccggc
     3361 catggccgcc ggcaagcagg tgattttgcc aaagcccttg agccgtgcga gccgatgaaa
     3421 gcggaatcca acgaggccgg gaacaagacg ccaaaagaat cttgtctggc gaacatgctc
     3481 ggcgatcaat tgtttgagcc agagcgggtc cttccggtct ttttcgcgca gggtcccggc
     3541 gagatttttc cgctcggaaa cggcgagcgt tttcagataa tcatccacca gcacatgaac
     3601 cgccgcctgg ccgatcatct gccgtttctg gtttctcaaa acgccgcgca acgtggtgta
     3661 agcctcgaat gtatgcgatg ccccgggcgc cgtcatgatg cgcagcgggt tcacatcgcg
     3721 tgttagaaaa tcggtgcaga caatcgcctt gatgaaccca tcttcgcacg cgggcaagtc
     3781 cttcggcaga tggatgcggc gcgcggtttc ggcgcggatg caataaagct gggcgcacaa
     3841 ttgcgcttgg ccagcccgcg tcatttgccc ggcgcggagt gaaagccgtt cggcaaaaga
     3901 tttgcgccgc cttgccgcga tgtgcttgca aggcttgtcg gttgagatga cggcgtgggg
     3961 attgccttcg agagcagcaa ccatgttcca caacgcttcg gtttcgttga tcacaatgtc
     4021 cgcgtccatc aagaacaaga accgcgcatc tggcgcggaa agttgatgca cgaaacaatt
     4081 ccatgcatta atttttccgc gctccttcaa gtcgagatgg cggcacgaaa aaatctcgcg
     4141 gcacggatgc cgatgcgact ggtcggcaaa aattttcgtg acaacgtccg acgtgccgtc
     4201 cgtgcagccg ttggcgacgc aaacaatttc gcaacgcagc ccgcgctggg ccagtttgcc
     4261 gaacagcgtt tgccggaaga gcgattccaa agcccgcccg atcgacttct cctcgttcca
     4321 cgcgatgatg ccgatggaga cgtccatttg gcgcgcttct tcgccgcctg gaaaaccgtc
     4381 gtcccacgcg gatgcgcccc aactgattgg atccagtatc acgttcgtga taatccagcc
     4441 ggacggcgca attttaaagt aggtggaagc cccggaacca gtagaggaat gaccttatat
     4501 tttccagcct ttgcggatca cgatttacgc gcccttgcaa tgcaaccacc gcgtaaatcg
     4561 tgtgcctgat ccacaattgt tccgccaaat cacggatgcg cgtatcggcc catcaagctg
     4621 gcctgatcca ccacatggcc cttcatctca gtctccaacg ccttggaatc cgttccacgc
     4681 gggagcgaaa gaacggtatc aagcgcatac acgttgaaaa aatagtgatg ctcgccggaa
     4741 ggcggcttgg gaccgccata ttccaattcc ccgaaatcat tccttccctg cgttgccatc
     4801 accgggacgc tgttctcgtg gatgtccgct gtgcgcgggc tcatgttgaa caagacccaa
     4861 tgatggaacg tcccgctcgg cgcatccggg tcttccacca ccaacgccag gcttttggcg
     4921 cggctcggaa tatcgtcgat gtggagcggc ggactcacat tggcgtcgtc cttggtgaat
     4981 tttttcggaa tggtatcgtt ctccttaaaa gccgaactgc tgatcttcat gcgattctcc
     5041 ttttaatttt accggattca aaatccgtaa cgttaaattc gcccttcaaa aaaccgccgt
     5101 caagaggcag ttctgctttt ttaacccatg caaaatgcgc caaagcgaca tcaagcgaca
     5161 cggtcggcaa aaaagatttt gttcagttta gcgttaatta ccgttaatta gcgttaatta
     5221 gcgttaattt tccgatgctg gatgctggac gcccgctgaa cctcaatcac tcaaccctca
     5281 acaatcaact ctcaaccatt cagcactcca catcccccac cccccggggg gtattttgcg
     5341 cggaattatc tgaaactatc tgaaattatc cggaatttca accacttaca acttgagcca
     5401 tttcactcaa tttcaactaa accggatgaa aaaatattta tttttttatc ccgttttatc
     5461 ccggttgatc tgaattgaag cggaaccaag cggaaaaaac ttccgaagca aggtgaaaca
     5521 agcggaacaa cgtagctgaa aacgtgagtt ttcaggtcct tccggacggg ccatgttcgg
     5581 cgaagtcccc aaacgtccaa gcatgagaga accgagacgc gacttgcccg cgaaaaacac
     5641 gaaagtccgc gaaatccgca accttaaacg ttcaacctta aactttggac cattcccggc
     5701 agaacccgga acttggcttc atgatttctc gcgaagaccg cgaagtccgc aaaggtagcg
     5761 ccacacactc cgtgcgcgcc gctttcctta actcggaact tggcttgccc gcccgtgcgg
     5821 aagcacctta accacattag aatgtcaaag aacaacccgt catcaagggc gagacgggaa
     5881 acaatcaaaa accgcaaagg cagcaacggt gcattgcccg tgtggaaaca ccgccgtccg
     5941 ccaactcgga actcggaacg cggaacccgg aacttgtttg cctttccacc ctccaacctc
     6001 ccatatttca tgttcaacta tcatgc
//
LOCUS       DASZMM010000536         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_533545, whole genome shotgun sequence.
ACCESSION   DASZMM010000536 DASZMM010000000
VERSION     DASZMM010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533545"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VG754_12915"
     CDS             <1..459
                     /locus_tag="VG754_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HWF20164.1"
                     /translation="TEIIPCARKVASMWHYTTQKPSGDWNKPAFNDAVWNQGCGSFGT
                     TGTPGAVVRTKWSSEDIWLRREITLPTTSCTNLQFYVHHDEDVEIYVDGILAASEPGF
                     TTDYVSMEIKPKALELLKPGAKVVLALHCHQTVGGQNVDVALVNVVNPEQ"
     gene            814..>3596
                     /locus_tag="VG754_12920"
     CDS             814..>3596
                     /locus_tag="VG754_12920"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWF20165.1"
                     /translation="MQKLRTAIAFCAVLTALAQCALGVEPKADLRDFAVRTWSSSDGL
                     PDAPVTVVTQTRDGYLWIGTHAGLFRFDGLSFTETPLPKEGTNAEVSVTSLCEDNSGD
                     LWVGTEKQGVYCCQQNGPAEHFGTNEGLLNVTSLTLDNEGHVWMGTGRGVKRFDGQHF
                     TTFTTADGLQDNAVLNVYAARSGRVWITTAGGTCRFVNGKLDQFHFPAGEQELDQDFR
                     QVYEDREGNLFALCSTYIINLADEKRINYFPGEKSAATRTWNLCEGRGGRLWIGANGR
                     GVYCFDGTKFQLVSLNEGRWPNDVRTIFEDHEGNLWLGITGIGLVQLRPQTFTLLTEN
                     RGLPQGAATCVMSDESGRLFVGMESGGIYASVSDHFEPVKGQDRFLDQDLVSSLCALR
                     DGAIWAGTEGMGLYQIKNGRTAIYTTANGLEDDSILSLCAGNDGAIWAGTHFGALQRI
                     ADGRISSFPPINGMSETSITAMLAMPGNRFWIGTSSGDLIHAQNDFKNISPAQLSPRL
                     AGKAILGLCESTNGGLWIGTDGGGLGYVNKDVRRAWDAQDGLPDNVVWGLTEDDEANL
                     WLATPRGLCRISSDSINRALARPEPLKVKLVYETDQSTPKALRFGGPHAIRSAGGRLW
                     FALNSGPVGVDIRGRESDKPAPQVHIENLLVDNQPVKFSAEKATPATSDAHADILMLP
                     GNPKTLEFHFVGLSFDAPEKVRFRHKMDGIDADWVQNGTERQIGYGPLPSGNYVFHVT
                     ACNAEGIWNDKGASLAFIIPTPLWRQPWALGMGGLTATFLVVGMVRVVSHRRLRWRLE
                     HLEQQRAMERERIRIAQNMHDEIGSKLTKISYLSERAKTELRGAGKAEGKIDSIATTS
                     RELLKALDEIVWAVNPKNDSLEHLAAYFCQYAREYFQDTTVECDIRMQSKLPEMEMSA
                     EMRHNLFLAFEES"
BASE COUNT          803 a          950 c         1078 g          765 t
ORIGIN      
        1 acggagataa tcccctgcgc gcgcaaggtc gcttcgatgt ggcattacac gacgcagaaa
       61 ccgtcgggtg attggaacaa gccggcgttc aatgacgcgg tatggaacca gggttgcgga
      121 agttttggca cgacaggaac acccggcgcg gtggtccgga caaagtggag ttcggaggac
      181 atttggttgc ggcgcgagat cactcttccc accacgtcat gcaccaattt gcaattctac
      241 gttcatcatg atgaggacgt ggaaatctat gttgacggca ttttggcggc gtcagaaccg
      301 ggcttcacaa ccgactatgt ctcgatggaa atcaaaccca aggcattgga gttgctcaag
      361 ccgggggcaa aggtggtttt ggctcttcat tgccatcaga ccgtcggcgg ccaaaacgtg
      421 gatgtcgccc tggtcaacgt cgtgaatccc gagcagtgat gccgccctca gcgggcgcat
      481 ctcactattt tgcacgtttc tgggctcttg cgaattgtgt ttatgggccc ggccacatcg
      541 ctgtccccct caccccagcc ctctccccaa ggagaggggg gaccatttgg ccgctgctcg
      601 ataaatcgaa cgctctcggt ttgcaaccac gacgggatgc gttgctccct ctccccaaag
      661 gagagggccg gggtgagggg gaggaaatgt tcaaccactc cgttgtgcaa gaaactgaga
      721 tgcgcccgcc ctcagccttt ttcagctttc gttcttgatt gccggccatt ggaaggagtt
      781 aactgtaaaa ctgcctctca aagatgtcgg cgcatgcaga agcttcgaac agccattgcg
      841 ttttgcgcgg ttctgacggc cctggctcag tgcgccttgg gcgtcgaacc gaaagcagac
      901 ctgcgcgatt ttgctgttcg cacctggagc agttccgatg gtttgccgga cgcgccggtg
      961 accgtcgtca ctcaaacgcg cgatggttat ctctggatcg ggacccacgc cggacttttt
     1021 cgattcgacg ggttaagctt cactgaaacg ccgctgccca aggaaggaac caatgccgaa
     1081 gtttccgtca cttcattatg cgaggacaat tccggcgatt tgtgggtcgg caccgagaaa
     1141 caaggggttt actgctgcca gcaaaacggt cctgcggaac atttcgggac gaatgaagga
     1201 ttgctaaacg tcaccagcct gacactcgac aatgaaggac atgtttggat ggggaccggg
     1261 cgcggagtca agcgatttga cggccagcat ttcaccacct tcacgacggc ggacggattg
     1321 caggacaacg cggtcttgaa tgtctatgcc gcccgttcgg gccgggtctg gatcacgacg
     1381 gctggcggga catgccgctt tgtgaacggc aagctggacc aatttcattt cccggctggc
     1441 gaacaggaat tggaccagga ttttcgacag gtttatgaag accgggaggg caatctgttt
     1501 gccttgtgca gcacgtacat catcaacctg gctgacgaaa aacggatcaa ttattttccg
     1561 ggtgaaaaat cagccgccac gcgcacatgg aatttgtgcg aagggcgcgg aggacgattg
     1621 tggatcggcg cgaatggccg cggcgtttat tgttttgacg ggaccaaatt ccagcttgtc
     1681 tcgctcaacg aaggtcgttg gcccaacgac gttcggacca ttttcgaaga ccatgaagga
     1741 aatctttggc tgggcatcac aggaatcggt ttggtccaac ttcggccgca aaccttcacg
     1801 ctcctgactg aaaatcgcgg actgccgcaa ggagcggcga cgtgcgtcat gagtgatgaa
     1861 agcgggcgac ttttcgtcgg gatggaatcg ggaggaattt acgcaagcgt ttccgaccat
     1921 ttcgaacccg tcaaggggca agatcggttc ctggaccaag acttggtctc gtccctttgt
     1981 gcgctccgtg atggcgcgat ttgggccggc acggaaggca tgggccttta tcaaatcaaa
     2041 aatgggagga cggcgattta cacgacggcg aatggcttgg aggacgattc catcctcagt
     2101 ttgtgcgcgg gcaatgacgg tgcgatttgg gcgggaacac attttggcgc tctgcaacgg
     2161 attgccgatg gccgcatctc cagtttcccg ccaatcaatg gaatgtccga aacctccatc
     2221 acggccatgc tcgccatgcc gggcaaccga ttttggatcg gaaccagcag cggagatttg
     2281 attcacgcgc aaaatgattt caagaacatc agcccggcgc aattgtcgcc acggcttgcg
     2341 ggcaaggcga ttttgggatt atgcgaaagc accaacggcg gtttgtggat cggcacggat
     2401 ggaggaggat tgggttatgt gaacaaggat gtgcggcgcg cctgggatgc gcaagacggc
     2461 ctgccggaca atgtcgtgtg gggattgacc gaggatgacg aagccaatct ctggcttgcg
     2521 acgccaaggg gcttgtgccg catcagcagc gattccatca atcgcgcgct ggcccggcct
     2581 gaaccgctta aagtcaaact ggtctatgaa acggaccaat ccacgcccaa ggcgctgaga
     2641 ttcggcgggc cgcacgcgat ccgttctgct ggcggccgtt tatggtttgc gctgaacagc
     2701 ggcccggttg gagtcgatat tcggggacgc gaatctgaca aacccgcgcc gcaagtgcat
     2761 atcgaaaatc tattggtgga caatcaaccg gttaaatttt ccgctgagaa ggcaactccc
     2821 gcaaccagcg acgcccatgc ggatattctc atgcttcccg gcaatccgaa gacgctcgaa
     2881 ttccatttcg tcggactgag ttttgatgcg ccggaaaaag tgcgcttccg tcacaagatg
     2941 gacgggattg atgccgattg ggtgcaaaac ggaaccgaaa gacaaatcgg ttacgggccg
     3001 ctcccgagcg gcaattatgt ttttcacgtg acggcgtgca acgcggaagg catttggaac
     3061 gacaagggcg ccagccttgc tttcatcatt ccgacgcctt tgtggcggca accgtgggcg
     3121 ctgggcatgg gcgggttgac cgcgacattc ctggtcgtgg gcatggtgcg cgtggtttcg
     3181 caccggcggc tgcgatggcg tttggagcat ttggaacaac aacgcgccat ggagcgcgaa
     3241 cgcatccgca tcgcccaaaa catgcacgac gaaatcggtt ccaagctcac caagatttct
     3301 tacttgagcg aacgcgccaa gaccgaactt cgcggtgccg gcaaggcgga aggaaaaata
     3361 gattcgatcg ccaccacatc gcgggaattg ctcaaggcat tggatgaaat cgtttgggcc
     3421 gtgaatccga agaacgacag cttggaacat ctcgccgcgt acttctgcca gtacgcgagg
     3481 gaatattttc aggacaccac ggtggaatgc gacattcgca tgcaaagcaa gctgcctgag
     3541 atggagatgt cggcggaaat gcgccacaac ctgtttttag ctttcgagga gtcgtt
//
LOCUS       DASZMM010000537         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1870642, whole genome shotgun sequence.
ACCESSION   DASZMM010000537 DASZMM010000000
VERSION     DASZMM010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870642"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..388
                     /locus_tag="VG754_12925"
     CDS             215..388
                     /locus_tag="VG754_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20166.1"
                     /translation="MIAVNANIVRHGKKTPAKILLPANHTEAANGSAWGLVIAGGLLL
                     VAAGFIGLLVVML"
     gene            complement(412..693)
                     /locus_tag="VG754_12930"
     CDS             complement(412..693)
                     /locus_tag="VG754_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20167.1"
                     /translation="MKSAYELAMERLNKTSPTVKVTDQQKQELAEIDSKYAAKIAERE
                     LLVRDELRKASAKGDFETIEQLEKQLVSDRKSLEAEKEEKKGKIRQTKG"
     gene            complement(697..1137)
                     /locus_tag="VG754_12935"
     CDS             complement(697..1137)
                     /locus_tag="VG754_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF20168.1"
                     /translation="MSSPQEQYDDAMFDFSTADFDSAIAKLKAVLAQDPNHFDAQLSL
                     GMAYYRKGDYATAIAEGHKAEKMRPNEQLVHTNLSLFYMKSGDKKTAEHHGLQARLAS
                     WKGNMAPPGSAPESASDPELEMAKPKPPPVKADQKFPDMPWKKK"
     gene            1249..2043
                     /locus_tag="VG754_12940"
     CDS             1249..2043
                     /locus_tag="VG754_12940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWF20169.1"
                     /translation="MIKLISTDFDGTLFAEFENPPIPQHLQQLIGEMQGRGVKWVINT
                     GRDMSGLMEALARAHISIKPDYLVLVEREIHCHQDSHYMGWHDWNDACHRSHEELFVR
                     VRADLPRLVSWVESRFSATIYEDAYSPFCLIAGNNGDADVIHEYMENYCSEVPNLKVV
                     RNDVYARFCHANYNKGTALAEICKRLQIEAQHTLAAGDHLNDLPMLNCEFARYLVAPD
                     NAIPEVKETVKRQNGFVSGLSHGHGVAEGLRYFLKQTSNGLARQNP"
     gene            complement(2015..3139)
                     /locus_tag="VG754_12945"
     CDS             complement(2015..3139)
                     /locus_tag="VG754_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20170.1"
                     /translation="MPDNQIGAHNPDPNVPSQGTPGNPPPVPPPPALAQVPPAPGPVG
                     THTLDSFPVVALILIHFFTCGLFSFIWLGLMHGRLPKVREDDPSAGKAVGFCFIPFFN
                     LYWIFFSYRRLCLRVDEQRILYGLPPSNLTSMATAACIFQVIPYVNILGSITTLPIFA
                     GQMQYSVNQLVKTSATTAPKGALQALPATSKGMPVWAIIMLVCGVSIFPIAFFAGMLL
                     PALANAKAKAERINCVSNLKQIGLAFKVWGGDHGDLYPFNVSTNKGGTLEVCDRGADG
                     FDRNSCLDFQVLSNELAITKILVCPGDHSKAAALDFSQLQPMNVSYLVHSRTNVSETY
                     PNTVLAICPIHRNVLFSDGSVGQVSQAEMQLIMDSVAQDR"
     gene            3550..>4079
                     /locus_tag="VG754_12950"
     CDS             3550..>4079
                     /locus_tag="VG754_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWF20171.1"
                     /translation="MSDATLTAQPAGGTPKVSKISETIIRIAGNSQDGIQAIGGFLAR
                     LAGRSEQEVMTHMTIPATISGGPSIFQVRIGSGEVLSSGDEADVLLAFYQHSYEDHIG
                     SLKKGGIVLYDSDHVEPKPEWQKEYHHVGIPISSLTVEAIGGTAKDKGKNIYTLGLVA
                     KMFDLNLPKLEKLIGER"
BASE COUNT          945 a         1095 c         1068 g          971 t
ORIGIN      
        1 ggtcgtggtg gccttgtatc atttcgatct gttgcagaga ggagtcgcca aggcttgatt
       61 gaatgtgtgc gggaattggg cacgcaaaac cagattttgc cgcacaatca aaccacagca
      121 ttctctgatt catcgcaagc cattcgcatg aagctatttc catcaacccg gccaatcgtt
      181 ctgccagtgg cttgggatat gcttttaact ggccatgatt gcagtgaacg caaacatcgt
      241 tcggcatgga aagaaaacgc cggccaaaat ccttttgcct gccaatcaca ccgaggcggc
      301 aaatggaagc gcgtggggtt tggtgatcgc gggaggattg ctgcttgtcg cggcaggatt
      361 tatcggcctg cttgtcgtca tgttgtaagc caggctgagt ttaaaaaggc ttcaaccctt
      421 cgtctgccgg atttttccct tcttctcttc cttctccgct tcgagcgatt tgcggtcgct
      481 gaccaactgt ttctccagtt gctcgattgt ctcgaaatct cccttcgcgg aagccttccg
      541 caattcgtcg cggacgagca attcgcgctc ggcgatcttc gcggcgtatt tggaatcaat
      601 ctcggcgagt tcctgcttct gttggtcggt caccttgacg gtgggagacg ttttgttcaa
      661 acgctccatg gcgagttcgt aagcggattt cataaatcac tttttcttcc acggcatgtc
      721 gggaaacttc tggtctgctt tcaccggcgg cggtttgggc ttggccattt ccagctccgg
      781 atcggaagcg gattcgggcg cgcttcccgg cggcgccatg ttgcctttcc acgaagcgag
      841 acgcgcctgc aacccgtggt gctccgcggt tttcttgtcg ccgcttttca tgtaaaacaa
      901 cgaaagattg gtgtgaacca attgttcgtt gggccgcatc ttttccgctt tgtgaccttc
      961 ggcgattgcc gtcgcgtaat cgcctttgcg ataataagcc atgcccaacg aaagctgcgc
     1021 gtcgaagtgg ttcgggtcct gcgcgagcac ggccttcaac ttcgcgatgg cggaatcgaa
     1081 atccgccgtg ctgaaatcga acatcgcatc atcatattgc tcttgcggcg aagacataat
     1141 aaacttctta ccaatgaggg tatctcagca atatccgaat gtaaacatgg ggcatcgaaa
     1201 tgaaatgccc ctgctgaaaa ctcacgtttt cagctaccct ggatcaccgt gataaaatta
     1261 atttcaaccg attttgacgg cacgttgttt gccgagtttg aaaacccgcc catcccgcag
     1321 catttgcaac agctcatcgg cgagatgcaa gggcgcggcg tcaaatgggt catcaacacc
     1381 ggccgcgaca tgtccgggtt gatggaagcg ctcgctcgcg cgcatatctc cattaagccc
     1441 gattatctcg tgctcgttga gcgcgaaatc cattgccatc aggattcgca ttacatgggc
     1501 tggcacgatt ggaatgacgc gtgccatcgt tcgcatgaag aattgttcgt gcgcgtgcgc
     1561 gctgatctgc cgcggctcgt gagctgggtc gaatcgcgtt tcagcgcgac gatttacgaa
     1621 gacgcctatt cgcctttctg cctcatcgcg ggcaacaacg gcgatgccga cgtcatccac
     1681 gaatacatgg agaattattg cagcgaagtg cccaatttga aagttgtgcg caatgatgtt
     1741 tacgcgcgct tctgccatgc gaattacaac aaaggcaccg cgctggccga aatctgcaaa
     1801 cgccttcaaa tcgaagcgca acacaccctt gccgccggcg accatctgaa tgatttgccg
     1861 atgctcaact gtgaatttgc ccgctatctc gtcgcgcccg acaatgcgat tccggaagtc
     1921 aaagaaaccg tcaaacgtca aaacggtttc gtctccggac tttcacacgg ccatggcgtg
     1981 gcagaaggtt tgagatattt tttgaaacag acatctaacg gtcttgcgcg acagaatcca
     2041 tgatcagctg catttcggcc tgtgacacct gccctacgct tccgtcgctg aaaaggacat
     2101 ttcgatggat cgggcaaatg gcaaggactg tgttggggta agtctccgaa acattcgtcc
     2161 gggaatgcac caagtaggaa acattcatcg gctgcaactg gctgaaatcc agcgcggccg
     2221 ctttcgagtg gtcacccggg caaacgagta ttttcgtgat ggccaattcg ttggacagga
     2281 cttggaaatc aaggcacgaa tttctgtcaa agccatccgc tccccggtcg catacttcaa
     2341 gcgttccccc tttgttggtg ctgacgttga acggatacag atcgccatgg tctccgcccc
     2401 aaaccttgaa tgcgaggccg atctgtttca gattgctgac gcaattgatg cgttcggcct
     2461 tggctttcgc gtttgccaaa gcaggcagca acatgccggc aaaaaacgcg atgggaaaga
     2521 ttgaaacccc gcaaaccaac atgatgatgg cccataccgg cattcctttg gaagtcgccg
     2581 gcaatgcttg caaggcgccc ttcggcgcgg ttgtggcgct ggtcttcacc aactggttca
     2641 cgctgtattg catctgcccg gcgaaaatcg gcaaggtggt aatactgccg agaatgttga
     2701 cgtaagggat gacttggaaa atacacgccg ccgtggccat gctggtcaga ttgctcggcg
     2761 gaagcccgta gagaattcgc tgttcatcca cgcgcaagca caggcggcga tagctgaaga
     2821 atatccaata aagattaaaa aatggaatga agcagaagcc caccgccttg ccggcagacg
     2881 gatcgtcttc ccttactttg ggcagcctgc catgcatcaa gcccaaccaa atgaaagaga
     2941 aaagtccgca ggtgaaaaaa tgaatcagga tgagcgcgac cacgggaaag ctatccagag
     3001 tgtgcgtgcc gaccgggccg ggagcaggcg gaacttgcgc caacgcaggc ggtggtggaa
     3061 ccggcggtgg atttcccggc gtgccttgag acgggacgtt aggatccggg ttgtgcgctc
     3121 caatttggtt atctggcatg gtttctccgg ttatatggtg gttttttgcc gaattggatt
     3181 ccgtctgata attcaagcac ccgcaacaac ttcaaataat ttttttggca acccggctcg
     3241 gtggcgaggc tgcattggcc caccccgaag gtcaaacaga aaaatgttcg gaattggcca
     3301 gccagtacag attgggacat ctcttaatgc tcaaatgcag taaaattaga gttactaatt
     3361 tggcgacttt tccttatttg gataaatcgc atatttttta tatgtcacta catttcaagt
     3421 acatacaaat ctagtttacc attgcgtgcc tccgggcgta atgatagttt aatcctgcaa
     3481 cgaagccgcg ttgactgaaa accagtgaac gcggcagttt tgcctaacag tataaaaaaa
     3541 gaatctttca tgagcgatgc aactctgacc gcacagcccg caggcggcac ccccaaggtt
     3601 tccaagatct ccgaaaccat cattcgaatc gcgggcaatt cacaggacgg cattcaggcc
     3661 atcggcggtt ttctcgcccg gttggccggt cgcagcgaac aggaagtcat gacgcacatg
     3721 accatcccgg cgaccatctc cggcggtcct tccattttcc aagtgcgcat cggttccggg
     3781 gaagtcctca gttccggcga cgaagcggac gtgttgctgg ccttctacca gcattcctac
     3841 gaagaccata tcggctctct caaaaagggc ggcatcgtcc tctatgattc cgaccacgtt
     3901 gaacccaagc ccgagtggca gaaggaatat catcatgtcg gcatccccat ttcgagcctg
     3961 accgtcgagg cgattggcgg cacggccaag gacaagggca aaaacatcta cactctcggc
     4021 ctcgtcgcca agatgttcga tctgaacctt cccaagctgg agaaacttat cggcgaacg
//
LOCUS       DASZMM010000538         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2708936, whole genome shotgun sequence.
ACCESSION   DASZMM010000538 DASZMM010000000
VERSION     DASZMM010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708936"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..1569
                     /locus_tag="VG754_12955"
     CDS             472..1569
                     /locus_tag="VG754_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWF20172.1"
                     /translation="MNAEFTHKLGQVRSVIKANKAAGALLGLQANFAWLACGGEAHVA
                     LISDRAAGQLLVTPKGFFLLANGIEMPRLQDEALRGLGAGPLSFDWFEDDDAANALRK
                     VIDPAKVISDTGDLGTRAMPDAFAQLRYSLHPAEIKRYRELARRAEAAMNAACRALKP
                     GMSEFEGIGILAEHCWESDVTPVVLLAAFDERIRRYRHPLPTTKKLKRHAMLVLCARQ
                     GGLIASVTRLVHFGKLPAELRKKHDAVCAVDAAFIASTRVGTPVREVFRRGVAAYVEQ
                     GFADEWKLHHQGGPCGYRPRDYVGTPTAPGVVLENQAFAWNPSITGTKGEDTILATSK
                     GPQILTAAQDWPMLEVEHHGETFLRPDILIR"
BASE COUNT          354 a          476 c          522 g          388 t
ORIGIN      
        1 aaattgaaga atgaaggcgt ttttttcaaa ctgttccatc aggccacgga tggaaagctg
       61 ttccgtgtct ccagctataa ccccgtttat ccggtcatgg aatgccgcaa ggatgatttc
      121 gtctggatct atcccgttta tcaagtgacg aagaatgttt ggaagtgatt gaacaaaaaa
      181 cggaagcgga ctcgggttgc gtcgatatct tcaaagaaga actctggatt tgttttttgc
      241 aaccgctgcc agcacgcggc agcctcgtag gtgagggctt cgcttgccct atgggtgtat
      301 cgcctttcgt taggtttcgg gtccattgct tttcttccaa accccgatac acccgctctc
      361 tacgctttta cacaacatcc ttacgccacc gcctccaaac tcatggacgc actgcgcatt
      421 cccgccacct gcaaagttgc gcccaacctg ccaagctgct aactctttgc cgtgaacgct
      481 gaatttacac acaaattggg acaggtgcgc tcggtcatca aggcaaacaa ggccgcgggc
      541 gcgctgttgg ggttgcaggc gaatttcgca tggctcgcat gcggcggcga ggcgcatgtc
      601 gcgttgatct ccgaccgcgc cgccgggcaa ttgctggtga cgccaaaggg ttttttcctg
      661 ctcgccaacg gcatcgagat gccgcgcttg caggacgagg cgttgcgcgg tttgggagcg
      721 gggccgctga gctttgactg gttcgaggac gacgacgcgg ccaatgcgct gcgcaaagtg
      781 attgaccccg ccaaggtgat ttcggacacg ggcgatttgg gcacgcgcgc gatgccggac
      841 gcgttcgcgc agttgcgcta ttcgctgcat cctgcggaaa tcaaacggta ccgcgaattg
      901 gcccgtcgcg ccgaggcggc gatgaatgcc gcctgtcgcg cgcttaaacc gggcatgagc
      961 gaattcgagg gcatcggcat tttggcagag cattgctggg aatccgatgt gacgccggtg
     1021 gtgttgctgg cggctttcga cgaacgcatc cgccgttatc gccatccgct gccgacgacg
     1081 aaaaagctca agcgtcacgc gatgctggtg ttgtgcgcgc ggcaaggcgg gttgatcgcg
     1141 tcggtgacgc gtctggtgca tttcggcaag ttgccggcgg aattgaggaa aaagcatgac
     1201 gcggtttgcg cggtggacgc ggcgttcatt gcgagcacgc gagtcggcac gccggttcgc
     1261 gaagttttcc ggcgcggagt cgcggcttat gtggagcagg gttttgccga cgaatggaaa
     1321 ttgcatcatc aaggcgggcc gtgtggttat cgtcctcgtg attacgtcgg cacgccgact
     1381 gcgccgggtg tggtgcttga aaaccaggcg ttcgcgtgga acccgtccat caccggcaca
     1441 aagggcgagg acacgattct cgcgacgtcg aaaggcccgc aaatcctgac cgccgcgcaa
     1501 gattggccga tgctcgaagt ggagcatcat ggagaaacgt ttttgcgccc ggatattttg
     1561 ataagatgat gagaaaaacc ttcacaattt ttgagaagca agcatcctta ccttccatga
     1621 aaaagattct gctcgcggtg gccatggctg tgctgatgaa ttccctccag gcgcaaacgc
     1681 tccctgaaca atctgcctct tccggcaact tgatcatcgt caagctcgat tccgcgccgt
//
LOCUS       DASZMM010000539         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4905929, whole genome shotgun sequence.
ACCESSION   DASZMM010000539 DASZMM010000000
VERSION     DASZMM010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4905929"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1502
                     /locus_tag="VG754_12960"
     CDS             <1..>1502
                     /locus_tag="VG754_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWF20173.1"
                     /translation="EKQDFESFMVTPDKDFGQLVGPKTFIYKPSRMGEGVEVLGLPET
                     LLKWGIERPDQVIDVLALWGDTSDNIPGVPGIGEKTATKLIAQYGTVENLLTRTSELK
                     GKLKENLETFRDQALLSKKLATINCDTPCARNLDELKIRPRNDDAIKALFVEFEFNSL
                     GRRLYGDDFKAGRGFQSLAKPAAEKTTAEDAAIEELALASETEPSAQLELTPAVTNHK
                     TIAEVPHQYHLVADANARAKLIQTLRGLKSFCFDMQTTSRDAREAGLIGIAFSFDAHT
                     GHYAALASDAEQVAAVLEEFRPLFEDERIEKVGHNLKFDLSVLKWHGITARGKMFDTM
                     VAHSLIEPDMRHGLDYLSEVFLGYALTPMTKAASAESEQASLAAVAPEMVLENAAEQA
                     DVAWQLRAKLAPLLAEKGQERVFYEIESPLVPVLVDMEFEGVRVDAAALAEFAVQLSK
                     EMADHEGTIFQLAGSKFNLNSPRQLGQILFEVLKLPAPKKTKTGQYATNE"
BASE COUNT          355 a          393 c          460 g          294 t
ORIGIN      
        1 ggaaaaacag gatttcgaat cgttcatggt gacgccggac aaggatttcg ggcaattggt
       61 cgggccgaaa acgttcatct acaaaccgtc gcgcatgggc gaaggggtgg aagtgctcgg
      121 cctgccggaa acgctgctca agtggggcat cgaacgaccc gatcaggtga tcgacgtgct
      181 ggcgttgtgg ggcgacacct cggacaacat tcccggcgtg ccgggcatcg gtgaaaaaac
      241 ggcgacaaaa ctcatcgcgc aatatggcac ggtcgaaaat ttgctcacgc gcacttccga
      301 attgaagggc aagctcaagg aaaatttgga aactttccgc gaccaggcgc tcctttccaa
      361 aaagctcgcc accatcaatt gcgacacgcc ctgcgcccgc aatctggatg aactgaaaat
      421 ccgtccgcgc aatgatgacg cgatcaaggc attgttcgtc gagttcgagt tcaattcgct
      481 cggacgccgt ttgtacggtg acgatttcaa ggcgggtcgc gggtttcaat ctttggcaaa
      541 accagccgca gaaaaaacga cagccgaaga cgccgcaatt gaggaattgg cgctcgcttc
      601 cgaaacggaa ccgtccgcgc aacttgaact gacaccagcc gtcacgaatc acaagaccat
      661 cgccgaagtt ccgcatcaat atcatctcgt tgccgatgca aatgcgcgcg cgaagttgat
      721 ccaaactttg cgcggcttga aatcgttttg tttcgacatg cagacgacca gccgggacgc
      781 gcgtgaagcg ggattgatcg gcatcgcatt ttcgtttgac gcgcacacgg ggcattatgc
      841 ggcgttggcg tcggacgcgg aacaggttgc ggcggtgttg gaggaatttc gtccgctgtt
      901 cgaggacgag cgcatcgaga aggtcgggca caatttgaaa ttcgatttga gcgtgctgaa
      961 atggcacggc atcacggcgc gcggaaagat gttcgatacg atggtcgcgc acagcctgat
     1021 agagcccgac atgcggcacg gactggatta tctttccgaa gtttttctcg gctacgcatt
     1081 gacgccgatg acgaaagcag ccagcgcgga atcggagcag gccagtcttg ccgcggtcgc
     1141 gccggaaatg gttttggaaa atgccgccga gcaggcggat gtcgcctggc aactgcgcgc
     1201 gaagcttgcg ccgttgcttg cggagaaggg ccaggaacgg gtattttacg agattgaatc
     1261 gccgctggtc ccggtgctgg tggacatgga attcgaaggc gtgcgcgtgg atgcggcggc
     1321 gctggcggaa ttcgccgtgc aactttccaa ggaaatggcc gaccacgaag ggaccatttt
     1381 tcaactcgcg ggcagcaagt tcaacctgaa ttcgccgcgg caattgggac agattttgtt
     1441 tgaggtgttg aaattgcccg cgcccaagaa aaccaagacg ggccaatacg cgacgaacga
     1501 gc
//
LOCUS       DASZMM010000540         5266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2435845, whole genome shotgun sequence.
ACCESSION   DASZMM010000540 DASZMM010000000
VERSION     DASZMM010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2435845"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VG754_12965"
     CDS             <1..1135
                     /locus_tag="VG754_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF20174.1"
                     /translation="IFHLLEELALAVKNIWLHEQLAGNHAMMADIFRQLSSACVIVGQ
                     DLTIIHANKAARASFSKAARRSAELEFSDLPPALGGKVYQVLKTGTAIAPFKYHPGDA
                     PNALFNVSIVPFQKAGSVLPASALLMAEDLTQSEQLRKLELEAANLRLVTTMADRLAH
                     EIGNALVPLSTHQQLLKERYRDPEFRATLEEAMSDSIKRVSRLINQMRFLARDSASSM
                     EAFPMERLIEDAFEEAQKHQPVKASKLDYKNGTKPIIITGDRTALKHALSEVMLNALQ
                     ANPSDPKIGVAIHSESNGNGVADIQIEIQDNGAGFKAENTQKIPTPFFTTRNVGLGLG
                     LTVSRKIIERHNGKLEIVTSKPGASGLVRITLPLAPAEGFHIN"
     gene            1140..2393
                     /locus_tag="VG754_12970"
     CDS             1140..2393
                     /locus_tag="VG754_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HWF20175.1"
                     /translation="MQATGIELTATTSQLQKRTLLIVDDEEGPRQSLRVVFKDDYNLL
                     MANDGARAIELARQNRVDVAITDIRMVGMSGVELLEQLKAVDPGIEVIMLTAYETVDT
                     LRLALRLGACDYLNKPFDISTMRKAVANAVERRSLGEEIRNNSQQLQELQKELQHQKT
                     TEEITRTRGEIYASIIHDINGPLTVISGFIQLMSQRIGSHNRIEGEDLDFLKDRLKTI
                     TRQVTNCIEISRRYLSFLRQNSEDTPMVGVNQILTDLHQLIRVHPSVKNNEFKIEPLS
                     ADATVKINGTDLIQILLNLAVNGFQCTPDKHSVHISGRIMQHPVDLSKFADGPEDRVL
                     NREGFRNAPPLLTLSVKDNGPGIPADIMPKIFDPYFTTKSANQGTGLGLNVVQRLVKE
                     AKACLHLHSGPGQGATFTIYFPTSS"
     gene            complement(2397..2540)
                     /locus_tag="VG754_12975"
     CDS             complement(2397..2540)
                     /locus_tag="VG754_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20176.1"
                     /translation="MEMDASGQNKFLKKLNPELGFFIAPRFAFCEHLRKIEKRNLCEP
                     KTV"
     gene            2783..3481
                     /locus_tag="VG754_12980"
     CDS             2783..3481
                     /locus_tag="VG754_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF20177.1"
                     /translation="MQAIEQKKEFGAAVKSERIRLGLSQETLAERADLHRTYITDIER
                     GARNLSLETIYKIAAALGVSIGSLFARSDNRPPMDLSGSGSGSLVDVLLVEDDPHDVE
                     LTLEAFRQAGMTNRIHVVREGAAALDFIFCRRAYANRRIETNPKVILLDLNLPKVNGL
                     EVLRQVKADDRTRYVQVVVLTMSRQDEHVRTAMALGAAAYIVKPVDFDNFSKVTPKLS
                     LHWALLGSNLTGTP"
     gene            3572..4669
                     /locus_tag="VG754_12985"
     CDS             3572..4669
                     /locus_tag="VG754_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF20178.1"
                     /translation="MNDCLETLPDAIVVIDSSGCIIFANSQAETLFGYSPAELTGKML
                     EVLLPKRFRMGHVLQRSNYFGTPRARPLHAGLELHGLRKDGDEFPAEISLSPLKTVEG
                     VLVISTIRDMSESKRVEQALGEKNLALAMANKELEAFSYSISHDLRAPLRHINGYVEL
                     LKLSIGPELAEKPGHYLTEIVRSADQMGRLVDDLLNFSRMSRAEMKQDKVDLNVLAQT
                     ALSHLESETKNRNIQWKWGVLPSLKADPALIIQAFINLLSNAIKYTRPRDPAEIEVGC
                     LEGTGDEAVIFVRDNGVGFDMEYADKLFGVFQRLHLREEFEGSGIGLANVRRIISRHG
                     GRTWAEGEVGNGATFYFSLPTGQLKGSAQEE"
     gene            4773..>5266
                     /locus_tag="VG754_12990"
     CDS             4773..>5266
                     /locus_tag="VG754_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF20179.1"
                     /translation="MSLAKTIFCWLILLACARWAGAETAVSSNAPPTKTPSYTLWQTL
                     NLALKQNPDVLIAQKKLEEAAGAIVEARAGLLPNLTTWDNYQRFSSDYATLNGVMANN
                     RPYLWNVSVRLTETAYAGGAVVNQMGIAQLNKKSRLLDYQATVNKVMMDVRIAFYDVL
                     RSQAG"
BASE COUNT         1243 a         1433 c         1400 g         1190 t
ORIGIN      
        1 gattttccat ctgcttgaag aacttgcgct ggccgtcaag aatatctggc tccatgagca
       61 attggccggg aaccatgcca tgatggcgga tattttccgc cagttgagca gcgcttgcgt
      121 cattgtgggc caggacctga cgatcatcca cgccaacaag gcggcgcgcg cgtcattcag
      181 caaagcggcc cgtcgcagcg ccgagcttga gttcagcgat ctccctcccg ctctcggcgg
      241 caaggtttat caagtgttga agaccggcac ggccattgcg cctttcaaat atcatccggg
      301 cgatgcgccc aacgctcttt tcaacgtcag cattgtgccg ttccaaaaag cgggttcggt
      361 cctgcctgca tcggcgctgt tgatggcgga ggacctgacg caatccgaac aacttcgcaa
      421 attggaactg gaagctgcga acctgcgcct cgtcaccacc atggctgacc gattggccca
      481 tgaaattggc aacgcactgg tcccgctttc aacgcaccag caactcctca aggaacgcta
      541 ccgtgaccct gaattccgcg cgacgcttga agaggcgatg agcgacagca tcaaacgcgt
      601 ttcacggctc atcaaccaga tgcggttcct cgcacgggat tcggcgagtt ccatggaagc
      661 attcccgatg gaacggttga ttgaggacgc ctttgaagaa gcgcagaagc atcagccggt
      721 caaagcttcc aagcttgatt acaagaacgg caccaaaccg atcatcatca ctggcgaccg
      781 caccgcgctc aagcacgcct tgtcggaagt catgctcaac gcgctccagg caaatccttc
      841 cgatccaaaa atcggcgttg ccatccattc ggagagcaac ggaaacggtg tggctgatat
      901 ccagattgaa atccaggaca atggggcggg gttcaaggcg gaaaacacgc agaaaattcc
      961 cacgccgttt ttcaccacgc gcaatgtggg ccttggcctt ggtttgaccg tcagccggaa
     1021 aatcatcgaa cgccacaatg gcaagctgga aatcgtcact tccaagccgg gcgcatccgg
     1081 attggtgcgc atcacgttgc cacttgcacc tgcggagggt tttcatataa actagcagcg
     1141 tgcaagcaac tggcatcgaa ttgaccgcga ccaccagcca actccaaaaa aggacgttgt
     1201 tgatagtgga tgacgaagag gggccgcgtc agtcgttgcg agtggtattc aaggatgatt
     1261 acaacctgct catggccaat gacggcgcgc gcgccatcga gttggcccgt caaaaccgcg
     1321 tcgatgttgc catcaccgac atccgcatgg tcggcatgtc cggtgtcgag ttgctcgaac
     1381 aactcaaggc cgttgaccca ggcattgaag tcatcatgct caccgcttac gaaacggtgg
     1441 acacgttgcg gctggcgctt cgacttggcg cttgcgatta tctcaacaaa cccttcgaca
     1501 tctccaccat gcggaaagcc gtggccaacg cggtcgaacg gcgctcgctc ggcgaagaaa
     1561 tccgcaacaa ctcgcagcag ttgcaggaat tgcaaaaaga attgcagcac cagaaaacca
     1621 ccgaggagat cacccgcacc cgtggcgaga tttacgccag catcatccat gatatcaacg
     1681 gcccgctgac ggtcatctcc ggattcatcc agttgatgag ccagcgcatc ggttcgcaca
     1741 accggattga gggcgaagat ttggattttc tcaaggaccg cctcaagacc atcacccggc
     1801 aggtcacgaa ttgcattgag atttcccgcc gttatctgag ctttctgcga cagaattccg
     1861 aggacacgcc gatggtcggt gtcaaccaga tcttgaccga cttgcaccaa ctcattcgcg
     1921 tccatcccag cgtcaagaac aacgaattta agatcgagcc tttgtctgcc gacgcgaccg
     1981 tgaagatcaa cggcactgac ctcatccaaa ttttgctcaa cctcgcggtc aatggattcc
     2041 aatgcacgcc ggacaaacat tcggtgcata tcagcggacg catcatgcag catccggtgg
     2101 atctttcgaa atttgccgat gggccggagg accgcgtcct gaaccgtgaa gggttccgca
     2161 atgcgcctcc gttgctcacg ttgtcggtca aggacaatgg gccgggaatt cctgctgaca
     2221 taatgccgaa aatttttgat ccttatttca ccaccaagtc cgccaatcaa ggcacaggcc
     2281 ttggcttgaa tgtagtgcag cgtctggtca aggaagccaa ggcctgtctg caccttcatt
     2341 ccgggccagg ccagggcgcc accttcacaa tttattttcc gacttcatcg taaccttcaa
     2401 acggttttcg gctcgcagag gttgcgtttt tcaattttac ggaggtgttc gcaaaatgcg
     2461 aaacgcggcg cgataaagaa tcccaattca ggatttaatt tttttaagaa tttattttga
     2521 ccgcttgcgt ccatttccat tgttgaactt gctttgttga aacaaaactg tcccactgct
     2581 ttttatcaca atgacgggtg caaacaaacg cgtgaattat gaatgaattt taatctttca
     2641 gcaggaggaa tgattcaaat ggcgggttct ttgaacaccc gttgagacaa atgccgactt
     2701 ttgtattata ttcatgtgct gtcacttata gtctgtagat gcagaacgat tttcctttct
     2761 acactcacgg ccattcttcg aaatgcaggc cattgaacaa aaaaaagaat tcggagccgc
     2821 cgtcaaatcg gagcgcatcc gtttgggcct ttcacaggaa actttggcgg agcgcgctga
     2881 cttgcaccgc acgtacatca cggacatcga acggggcgcg cgcaatcttt cactggagac
     2941 gatttacaaa atcgccgcag cgctcggtgt ttccataggt tcgctcttcg cgcgctctga
     3001 taatcgtccg ccgatggact tgtccggttc gggctccggc agccttgttg atgtgctgct
     3061 tgtcgaagac gatccacacg atgtcgaatt gacattggaa gcatttcgtc aggcgggcat
     3121 gaccaaccgc atccatgtcg tccgcgaagg cgctgcggcg ctggacttca tcttttgccg
     3181 tcgcgcgtat gcgaatcgcc gcatcgaaac aaacccgaag gtgatattgc tggatttgaa
     3241 tttgccgaag gtcaacggat tggaagtgct gcgtcaggtc aaggcggacg accgcacgcg
     3301 ttatgtgcag gtggttgtgc tcactatgtc gcgtcaggac gagcatgtgc gcacggcgat
     3361 ggctttgggg gccgctgcgt atatcgtcaa accagtggac tttgacaatt tcagcaaagt
     3421 caccccaaag ctcagtttgc attgggcttt gctgggttca aatttgaccg gaacgccgtg
     3481 aacagcatca tacaccctct gccagcagtt cgaagcgcag atggggcatg gacagaccgc
     3541 agccaaggtt atgcggaaat tatcgcctga gttgaacgat tgtctggaga ctttgcctga
     3601 cgccatcgtc gtcattgatt ccagcggttg catcatcttt gccaattcgc aggctgaaac
     3661 ccttttcggc tattcgcccg ccgagttgac cgggaagatg ctggaggtgc ttttgcccaa
     3721 acggttccgc atgggccatg tcctgcaacg ttccaattat ttcggcacac ctcgcgcccg
     3781 ccctctccat gcgggcttgg aactccatgg cttgcggaaa gatggggacg aatttcccgc
     3841 tgaaatcagt ctcagcccgc ttaaaaccgt ggaaggggta ctggtcatca gcaccatccg
     3901 ggacatgagc gaaagcaaac gggttgagca ggcattgggt gaaaaaaatc ttgccttggc
     3961 catggcgaac aaggaattgg aggcgttcag ctattccatt tcccacgacc ttcgcgcacc
     4021 gctccggcac atcaatggtt acgtagaact gctcaaattg agcatcggtc cggaacttgc
     4081 ggaaaagccc ggccattacc tgacggaaat tgtccggtcc gccgaccaaa tgggacggct
     4141 tgtggatgat ctgctcaact tttcccgcat gagtcgcgcg gaaatgaaac aggacaaagt
     4201 ggatttgaat gtgctggcgc agactgcgtt gtcccatctc gaatcagaaa cgaaaaaccg
     4261 gaacatccaa tggaagtggg gcgtgctgcc gagccttaaa gctgaccccg cgctcatcat
     4321 ccaggcattc atcaatctgc tttccaatgc catcaagtac acccggcccc gcgacccggc
     4381 tgaaatcgaa gttggttgtt tggaaggaac cggtgatgaa gctgtcattt ttgtgcgcga
     4441 caacggcgtc gggtttgata tggaatacgc cgacaaactt tttggtgtat ttcagcgatt
     4501 gcacctgcgc gaagaattcg aaggctcggg catcggtctg gccaatgtcc gccgcatcat
     4561 ttcacgccac ggcgggcgaa cttgggccga gggcgaggtt gggaacggcg ccacttttta
     4621 tttttcgttg ccaacaggtc aattgaaagg gagtgcgcag gaagagtaag gacgtgccaa
     4681 tttagtattt ttggcccggt ttcattggat gatttccttc ctttaagaaa agtcattggc
     4741 aagaggttcg gttgtgttag gattcggcca atttgtctct cgcaaaaaca attttttgtt
     4801 ggctgatttt gcttgcttgc gcccgatggg ccggggcgga gaccgcagtt tcctccaacg
     4861 cgccgcccac aaaaacgcca agttacacgc tctggcaaac gctcaatctc gccttgaaac
     4921 aaaatcccga tgtgctcatc gcccagaaga aattggaaga agccgcgggg gcaatcgtcg
     4981 aggcgcgcgc gggacttttg ccaaacctga caacctggga caattaccag cgtttttcat
     5041 ctgattacgc gaccctgaac ggagtgatgg cgaacaaccg gccatatctc tggaatgttt
     5101 cggtgcggct tacggaaacc gcctacgctg gtggcgcggt ggtgaaccaa atgggcatcg
     5161 cccaactcaa caagaagtcg cgcctgctcg attatcaagc gaccgtcaac aaggtgatga
     5221 tggacgtgcg catcgctttt tacgatgtgt tgagaagcca ggcggg
//
LOCUS       DASZMM010000541         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5180300, whole genome shotgun sequence.
ACCESSION   DASZMM010000541 DASZMM010000000
VERSION     DASZMM010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5180300"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VG754_12995"
     CDS             <1..603
                     /locus_tag="VG754_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HWF20180.1"
                     /translation="TAVLFFTGTTWALGSDGKITFMDGVVLVGLFLFWQCFHVFEVLK
                     SNAQQNKRFSWMLPVDLALLAVGAYAVYASTDWLVDWISKIHTGFISAKYMGWLSGWL
                     MVLPNALLAIYYGSTNRPEVVYTSQVGDNHICIPLCIGVYAMFKTIHVPAFFQPGVLV
                     LLGATLLHFFFIASFGRLPRLAGLALIAAYGYFIYTGLLK"
     gene            complement(605..1030)
                     /locus_tag="VG754_13000"
     CDS             complement(605..1030)
                     /locus_tag="VG754_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20181.1"
                     /translation="MNLDQLTKAIKQCAEQMNSRYKKVVFDEWAIVSLAQKKSRILNY
                     IGPRNDDFLKNFANDMGALRAELLNAQRGAGDFEFTRHGVGTLFEAFMVLGDGFYLIC
                     NHTSNSMDGLTRDPHWIDAQVPFVELSDKVRANPLVYTF"
     gene            1193..1915
                     /locus_tag="VG754_13005"
     CDS             1193..1915
                     /locus_tag="VG754_13005"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HWF20182.1"
                     /translation="MEEPRVVQCVMILALAATLLRLARTLPWQNVIACAVLTGALAGI
                     FQGVGARTGIPFGPFFYTENIGHLVFHEVPWPLPLLYIAILLNVRDVARLILWRWRET
                     TNYGVWVISLASLLATVFDAGLEPIASTGHWWIWTTSKSIPSWQGAPWINFAGWIATS
                     FFILALATPWLINKKTSSQSPRDYFSLIIWVILTLLLAVNNASHKYWMTAALTVAVDI
                     VLVVLALRNSNVPSQVAKKSSH"
     gene            complement(1917..>3651)
                     /locus_tag="VG754_13010"
     CDS             complement(1917..>3651)
                     /locus_tag="VG754_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20183.1"
                     /translation="SWRKWPDPLVDYGPFSVCLNAMLFKVFGPGLMVLVMTNLVVYAL
                     ILVLAYLAFRMAWGRLGAFAALAVFISAFSFSHLLGVGNYNFVTPYAAEASHGMLLIL
                     ATAFIVVRWCRNPSRKLAFMLGLCGGLAAVMKPEFMLACGLLGIAALVLRWRNRVPVT
                     AKEYRAMGVGVLLPTLVFTLWFARGESFQQAFIDASQAWWIVLVDRVQTGNPQQMGFL
                     GLDNPWANALKEIRCAGLAILILGAIWTIGRMMNLPWTRRSRLILLLGTTALIAAVFV
                     SGEWKIIRDWLSSLNKDWISVGLCLPGLMVLISVMLLVRLVRECRQTRQITERNVMAF
                     ALVLLAVAMLARMLLNVRVYHFGFFQAALAAMVVAAVMVSELPRWTGEKVLGRGLARI
                     GCLLILGLACGTIAAKSLAIRADQTEPVGSGRDRFYSTKHTIDGTGALVNWAAVRMSS
                     VPSDAEVLVLPEGMMINYLSRHKSPESAVLRESEEKFLEQLRRKPPEYIILISRDIKD
                     FGITRYGAPGNPGSALVPWVGANYKSEQWVGGDPLDPNAQVGAVIMRRKTDASPVSVS
                     KKSSEPASKKE"
BASE COUNT          840 a         1058 c          924 g          829 t
ORIGIN      
        1 acggcagtgt tgtttttcac cggcacaacc tgggcgcttg gttcggacgg caaaatcact
       61 ttcatggacg gcgtggtgct ggtgggattg tttttattct ggcaatgttt ccacgttttc
      121 gaggtgctta aatcgaatgc gcagcagaac aaacgtttta gttggatgtt gcccgtggat
      181 ttggcgctgc tggcggtcgg tgcttatgcc gtttacgcca gcaccgactg gctggtggac
      241 tggatctcca agatccatac cggcttcatc agcgcgaaat acatgggctg gctcagcggc
      301 tggctgatgg tgttgcccaa cgctctgctg gcgatttatt acggttcgac caaccggccc
      361 gaagtcgtct atacgtccca ggtcggcgac aaccatattt gcatcccgct ttgcatcgga
      421 gtttacgcga tgttcaaaac gatccatgtg ccggctttct ttcaaccggg cgtcctcgtg
      481 ttgcttggcg cgacgctgct tcactttttc ttcatcgcga gctttgggcg tttgccgcgg
      541 ctggcaggac tggcgctcat cgcggcttac ggttatttta tttataccgg cctgctgaaa
      601 tgattcagaa agtataaacc agcggattgg cccgcacctt gtcgctcaat tcgacaaacg
      661 gcacctgtgc atcaatccaa tgaggatcgc gcgtgagtcc atccatcgaa ttgctcgtgt
      721 gattgcagat cagataaaac ccgtcgccca gaaccatgaa tgcctcgaat aacgtgccga
      781 caccatgacg cgtgaattca aaatcccctg ccccgcgctg cgcgttgagc aattccgcgc
      841 gcaacgcacc catgtcattg gcgaaatttt tcaaaaaatc gtcattgcgc ggcccgatgt
      901 aattcaagat gcgactcttc ttttgggcaa gcgagacgat ggcccattcg tcaaacacga
      961 ctttcttgta gcgggaattc atctgctccg cgcattgctt gatggctttg gtgagttggt
     1021 caagattcat atcatgtttc agcttccgcg cgccgggcgc gatggtttcg tcccaaaacg
     1081 aggcagttca tccggctgtt ggacagaact gcgcgcccca aaccacccgg tccgcgaaat
     1141 ttatttacgg ctttcagccg gatggattgg agctactgtt ataccacttt tgatggaaga
     1201 accgcgcgtc gtccaatgcg tcatgatcct cgcccttgcc gccaccttgt tgcggctggc
     1261 gcggacgttg ccatggcaaa atgtcatcgc gtgcgccgtt ttgacgggag cgcttgccgg
     1321 aatctttcaa ggcgttggcg caaggacggg aatcccgttc gggcctttct tttacacgga
     1381 aaacatcggc catttggttt ttcatgaagt gccatggccg ctgccgttgc tttatattgc
     1441 cattctgctg aacgtccgcg atgtcgcgcg gctcattctt tggcgatggc gtgaaacgac
     1501 gaattacggc gtatgggtca tcagccttgc ctcgctgctt gcaacagttt tcgatgccgg
     1561 tctggagcca attgcctcca ctggccattg gtggatttgg acgacctcga aaagcatccc
     1621 ttcgtggcaa ggcgcgccat ggatcaattt cgccgggtgg attgccacaa gctttttcat
     1681 ccttgcgctg gccacgccgt ggctcatcaa caaaaaaacg tccagccaat cgccgcgaga
     1741 ttatttttct ctcataatat gggtaatcct tacccttctt ctcgcagtca acaatgcttc
     1801 tcacaaatat tggatgactg ctgctttgac agttgccgtt gatattgttc tggtcgtttt
     1861 agcattgcgg aattctaatg tcccatcgca ggtcgctaaa aaatcgagcc attaaatcat
     1921 tccttcttag atgcgggttc gctcgatttt ttcgacactg aaaccgggct cgcatccgtt
     1981 ttccgtcgca tgatgaccgc gccgacctgg gcatttggat ccagcggatc accgccgacc
     2041 cattgttcgg atttgtaatt cgccccgacc catgggacaa gggcagatcc gggattgccc
     2101 ggcgcgccgt agcgcgtgat gccaaaatcc tttatgtcgc gggaaatcag gatgatgtat
     2161 tcgggcggtt tgcgacgcag ttgttcgaga aatttttcct ccgattcgcg cagcacagcg
     2221 ctttccggac ttttgtgacg cgaaagataa ttgatcatca ttccctccgg caaaacgagc
     2281 acctcggcgt ccgaaggcac tgaactcatc cgaaccgcgg cccaattgac cagcgcgccg
     2341 gtgccgtcaa tcgtatgttt cgttgaataa aaccggtcgc gtccactgcc gaccggctcg
     2401 gtttgatcgg cgcgaatggc gagcgatttg gcagcgattg tcccgcaagc aagacccagg
     2461 atgagcagac aaccaattct tgcaaggccg cgccctaaaa ctttttcgcc ggtccaacgc
     2521 ggcaattcgg acaccatcac agccgcaacg accatcgcgg caagcgcggc ctgaaaaaaa
     2581 ccaaaatgat aaactcgaac gttcagcagc attcgggcga gcatcgcgac cgccagcagc
     2641 accaacgcaa acgccatcac gttgcgttcc gttatctggc gggtttgccg acattcacgg
     2701 accaatcgaa ccaacaacat aaccgaaatc aacaccatca aaccgggaag acaaaggccg
     2761 acggaaatcc aatccttgtt gagactgctc aaccaatcac ggatgatctt ccattctccg
     2821 ctgacgaaaa cagcagcaat gagcgcagtc gttcctagca gcaagatcaa ccgcgagcgt
     2881 cgcgtccacg ggagattcat catccgccca attgtccata tcgcgcccag aatcaaaatt
     2941 gccaacccgg cacaacggat ttccttcaag gcattggccc agggattgtc caagcccaga
     3001 aagcccattt gctgcgggtt cccggtctgc acgcggtcca ctaaaacaat ccaccatgcc
     3061 tggctcgcgt caatgaatgc ctgttggaac gattcgccgc gcgcgaacca aagcgtgaag
     3121 acaagcgtcg gcaacagaac gcccacaccc atcgcccggt attcttttgc ggtgacagga
     3181 acccgattcc gccagcgcaa taccagcgcc gcaattccaa gcaacccgca cgcgagcatg
     3241 aattcgggtt tcatcaccgc cgccagaccg ccgcacaagc cgagcataaa cgcaagcttg
     3301 cgcgaaggat tgcgacacca tctgaccacg atgaacgctg tcgccaaaat caggagcata
     3361 ccgtgcgaag cttccgcagc gtaaggcgtc acgaaattgt aattgcccac tccaagcagg
     3421 tgagaaaacg aaaatgccga aataaaaact gccaacgccg caaatgctcc caatcgtccc
     3481 cacgccatcc ggaaagcgag atacgccagc acaaggatca gggcataaac aaccaagttc
     3541 gtcatcacca gcaccatcaa tcccggcccg aaaactttga acagcatcgc gttaaggcag
     3601 accgagaacg gcccgtaatc aacgagcggg tcgggccatt tgcgccagga a
//
LOCUS       DASZMM010000542         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2155419, whole genome shotgun sequence.
ACCESSION   DASZMM010000542 DASZMM010000000
VERSION     DASZMM010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155419"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VG754_13015"
     CDS             <1..1065
                     /locus_tag="VG754_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HWF20184.1"
                     /translation="LGPTSDDLTRDLIAQLLGKKLHSDATILKHLENFFAQRNRPMPE
                     TTKVQAQVPEGALVLDNPHGTAPGLAMEISPNPFRDNAKPSWLVMLPGPPREMRPMFT
                     NHVLPLLQKNFPLDTGFVCRTLRTTGLGESIVEEKISGALQPLVKAGLELGYCAHYGE
                     VDVRLVARGAHAEKTVAEGETIVREKLGPHIFGLQDDQLNSTIVQLLTERKQTLVLAE
                     SCTGGYVANRITDVPGASTVFLGGMVTYSNESKQNLLGVRAQTLAQHGAVSEPTAREM
                     AEGARKRFNADYALAITGIAGPTGGTPTKPVGTVFIALATANHTFVLNPINRFDRPTF
                     KQVTCQQALDFLRRVMLKTQ"
     gene            complement(1206..2330)
                     /locus_tag="VG754_13020"
     CDS             complement(1206..2330)
                     /locus_tag="VG754_13020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWF20185.1"
                     /translation="MKLNSKFLVPLCAVAISAAAVITATVLRAEDKTAATNAPAKAGG
                     STSKIEDLFPDPVIARGTGIEIKRSALDEAVSSIRASATAHGQQIPPDLMPRIEKESF
                     DHLLQVKLLKDKATDADKAAGKIEGDKRMEGIRKRAPSEDILVRQLKALNLTVDELHS
                     RLTEEATAEEVLRDKVTVTDADVKKFYEDNPSKFEEPEKVKVSHILVATGDPRSPMSD
                     DAKKAKLKVAEGLLKRAKANEDFGKLVKEYSEDPGSKDKGGEYTFGHGEMIKEFEAAA
                     FSLQTNQISDIVTTAYGYHIIKLLEKIPAKKLAFADTKPDIKNYLERMQMEKILPDYY
                     VTLKKEAKVQILDDQLKALEDAPMDLPKGGIAPSATVAPK"
     gene            complement(2466..4046)
                     /gene="serA"
                     /locus_tag="VG754_13025"
     CDS             complement(2466..4046)
                     /gene="serA"
                     /locus_tag="VG754_13025"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWF20186.1"
                     /translation="MICDPISPKGIALFQQCKDFNVTVLKQRLSEAELLPLVKDVEAM
                     VVRSETKITRAVIEAAPKLRVVGRAGVGVDNVDVEAATQRGIVVMNTPSGNTISTAEL
                     TFSMLMSLARKIPQANASMKAGEWNRKAFQGVELYNKTLGILGMGRIGTEVARRAIVF
                     GMRVLAYDPYLTISRANALQVELVELDEIFTRCDFITVHMPMSEETRGMINAAAFAKM
                     KPNARVLNCARGGIINEADLYEAVKSGKIAGAALDVYETEPLPKDFPLRDLPQVIMTP
                     HLGASTEEAQENVGIEVAEAITDYLLNGAVRNAVNLPNLDAKTYAQVKPYLALGEKLG
                     RLAAQLAPKRNDRLVVTYGGKATEAPSDPITRFVLKGFLESAGGKDVNQVNVRTLAEG
                     LGLRVEEIKSNEETDFNEWLHLAVYSGEQKVSVGGTLYGSKSQPRIVRVNSQPVEIVP
                     EGVLLFLTNKDRPGIVGHLGTLLGRHKVNIASMSLSRDIAGGHALTVLNLDSVPPAGV
                     LDELQKDPDISNVRVVKL"
BASE COUNT          904 a         1440 c         1121 g          941 t
ORIGIN      
        1 ctcggcccga cctccgatga tctcacacgc gatttgatcg cccaattgct cggcaaaaaa
       61 ttgcattcgg acgcaaccat cctcaaacat ctggaaaatt tcttcgccca acgcaaccgt
      121 cccatgccgg aaacgaccaa agtccaggcg caagttcccg aaggcgcgct tgtgctcgac
      181 aatccgcacg gcaccgcgcc cggtcttgcg atggaaatca gtcccaaccc tttccgcgac
      241 aatgccaaac cttcctggct tgtcatgctg cccgggccgc cgcgcgaaat gcggccgatg
      301 ttcaccaatc acgtccttcc gctgctgcaa aaaaatttcc cgctcgacac tggcttcgtc
      361 tgccgcacct tgcgaacaac cggtctgggc gaatccatcg tcgaggaaaa aatttccggc
      421 gcgctgcagc cgttggtcaa ggccggcctg gaacttggtt attgcgcaca ttacggcgag
      481 gtggatgtgc ggctcgtcgc ccgcggcgcg cacgcggaga agaccgttgc cgagggcgaa
      541 accatcgttc gcgaaaaact cggtccgcac attttcggcc tgcaggacga ccagcttaat
      601 tcgaccatcg tccagcttct caccgaacga aagcaaaccc tcgtcctcgc cgaatcctgc
      661 accggcggtt atgtggcgaa ccgcatcacc gatgttcccg gcgcgtcgac ggtttttctc
      721 ggcggcatgg tcacttacag caacgaatcg aagcaaaacc tgctcggcgt ccgcgcgcag
      781 acacttgccc aacacggcgc agtcagcgaa ccgaccgcgc gcgaaatggc cgaaggcgcg
      841 cgcaagcgtt tcaacgctga ttacgcgttg gccatcaccg gaatcgccgg cccaaccggc
      901 ggcacgccaa cgaaaccggt cggcacggtt ttcatcgcgc tcgcaacggc caaccacacc
      961 tttgtgctga acccgatcaa ccgctttgac cgccccacgt tcaaacaagt gacgtgtcaa
     1021 caagcgctgg attttttgcg gcgcgtgatg ctgaaaacac aatgagaaag ttcttttaaa
     1081 aagtggagcg cggctgtgcg cgaagcgtca gccgcagcaa ggtaaagaag tcctgatgtt
     1141 tgaagcccct gcggctggtc cttgcgaaca cagccgcgat ccgttccccg ctctccgccg
     1201 aaaatttatt tcggcgcgac tgtggcggac ggcgcgatgc cacctttggg caaatccatc
     1261 ggcgcgtctt ccaacgcctt caactggtcg tcgagtatct gcactttcgc ctctttcttg
     1321 agagtgacgt agtagtcagg caggattttt tccatctgca tccgctccag gtaattcttg
     1381 atgtccggct tcgtgtccgc aaatgcaagc ttcttggcgg gaattttttc cagcagcttg
     1441 atgatgtgat agccataagc cgttgtgacg atgtcgctga tctggttcgt ctggagcgag
     1501 aacgcggccg cttcgaattc tttgatcatt tcgccgtggc cgaacgtgta ttcgccgccc
     1561 ttgtccttcg acccgggatc ctccgaatat tccttgacca gctttccgaa atcttcattc
     1621 gctttcgccc gcttcaacaa accctcggca actttcaact tcgccttctt cgcgtcgtcc
     1681 gacatcgggc tgcgcgggtc gcccgtggca accaggatgt ggctgacctt caccttctcc
     1741 ggttcctcga acttggaagg attatcctcg taaaactttt tcacgtccgc gtccgtgacg
     1801 gtgaccttgt cgcgcaaaac ctcctccgcg gtggcttcct cggtgagccg gctatgaagc
     1861 tcatcgaccg tgaggttcaa cgccttcaac tggcggacca aaatgtcctc gctcggcgcg
     1921 cgcttgcgga tgccttccat ccgtttatcg ccttcgatct tgccggcggc cttgtcggcg
     1981 tcggtcgcct tgtccttcaa aagcttcacc tgcaacagat gatcgaaaga ttccttttca
     2041 atccttggca tcaggtcggg cggaatctgc tggccatgcg ccgtggcaga agcgcgaatg
     2101 ctggaaacag cctcgtcaag ggcgctgcgc ttgatttcaa ttccggtgcc gcgcgcgatg
     2161 acgggatccg gaaacaaatc ctcgatcttc gaagtggaac cgcctgcctt cgcgggagcg
     2221 ttcgttgcgg ccgttttgtc ttccgcccgc aacacagtcg ccgtgatgac cgcggctgcg
     2281 ctgatcgcca ccgcacaaag tgggacaaga aatttgctgt tcaatttcat ataaaaattt
     2341 tcgctgtatt ccgactttcc acatcgcggg actggaaagt cccgccaacc cgcagcctgg
     2401 aaaggctgcg ctactgtagc gcaggcattc ctgcctgcgg gttctggcgg ctttccagcc
     2461 gccagttaca acttcaccac ccgcacattg ctgatgtccg ggtctttctg caattcatcc
     2521 agcacgcccg ctggcggaac gctgtccaaa ttcaaaaccg tcaacgcgtg gccgcccgca
     2581 atatcgcggc tcaagctcat gctggcaatg ttcactttat gccgtcccaa caacgtcccc
     2641 aaatgaccca caatgcccgg acggtccttg ttcgtcagga acaaaagcac gccctccggc
     2701 acgatctcca ccggctggct gttcacccgc acgatgcgcg gctggctctt cgaaccataa
     2761 agcgtgccgc ccacagaaac tttttgttcc ccgctgtaaa ccgcgagatg cagccactcg
     2821 ttgaaatccg tctcttcgtt cgacttgatt tcttccaccc gcaacccaag cccctcggca
     2881 agcgtccgca cattcacctg gttcacgtcc ttgccgccgg ccgattccaa aaatcccttg
     2941 agcacgaacc gcgtgatggg gtcgctgggc gcctcggtgg ccttgccacc gtaagtcacc
     3001 acgagacggt cgttgcgctt gggcgcgagc tgggcggcca aacgccccaa tttctcgccc
     3061 aacgcaagat aaggtttcac ctgcgcgtac gtcttggcat caagatttgg taaattgacc
     3121 gcgttgcgca ccgccccgtt caataaatag tccgtgatcg cctccgccac ttcgatgccc
     3181 acattctcct gcgcctcctc cgtgctcgcg cccaaatgcg gcgtcatgat cacctgcggc
     3241 aaatcccgca acggaaaatc cttcggcaac ggctccgttt cataaacgtc caacgccgcc
     3301 cccgcgatct tcccgctctt caccgcctcg taaaggtccg cctcgttgat gattcccccg
     3361 cgcgcgcaat tcaacacccg cgcattcggc ttcatcttcg cgaacgccgc cgcgttgatc
     3421 atcccgcgcg tctcctcgct catcggcatg tgcaccgtga tgaaatcgca acgcgtaaaa
     3481 atctcgtcca actccaccaa ctcgacctgc aacgcgttcg cccgcgaaat cgtcagatac
     3541 ggatcatacg ccaaaacgcg catcccgaaa acaatcgccc gccgcgcaac ttccgtcccg
     3601 atgcgcccca tgccgaggat gcccagcgtc ttgttgtaca gctcgacccc ctggaacgcc
     3661 ttgcggttcc attcgcccgc tttcatcgag gcgttcgcct gcggaatctt tcgcgccagc
     3721 gacatcaaca tcgagaaagt caattcagcc gtcgagatgg tgttgccact cggcgtgttc
     3781 atcaccacga tgccccgttg cgtggctgcc tcgacatcca cattatccac gcccacaccg
     3841 gcgcggccca ccacgcggag cttcggcgcg gcttcgatca ccgcgcgggt gatcttcgtc
     3901 tcggaccgca ccaccatcgc ttcgacatct ttgaccaggg gcaaaagttc agcctcagac
     3961 agccgctgct tcagcacagt gacattgaaa tctttgcact gctgaaacaa ggcgatgccc
     4021 ttcggcgaaa tcggatcgca aatcatcaca ttattcataa cgacaggggg cgaagaatag
     4081 gctacgaggg caagcaggtc aaatagggaa aaaggggtgc atccgacgct taaaagttcc
     4141 gctcggcggc tattttctat cgaattggga aattcaaacc atacggcgca aattccagcg
     4201 agtgccacgc gggcccagac ctctggggaa acaaatcgaa atattcagcg cctgaacggt
     4261 ggcatgggcg catctcagtt tttagagggg tcgatgcgcc gatgatgggg aaagagaagg
     4321 tgccagcggc cattgcgcca gaaggtttcc aagcatagca gcgcctcatc gccttgctgg
     4381 ctccagtatt gccccggccg cttgaa
//
LOCUS       DASZMM010000543         5354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4375507, whole genome shotgun sequence.
ACCESSION   DASZMM010000543 DASZMM010000000
VERSION     DASZMM010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4375507"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VG754_13030"
     CDS             complement(<1..1319)
                     /locus_tag="VG754_13030"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWF20187.1"
                     /translation="MLRAANRSFVAVLLLLWQLTGSVFASVSETQSVSKEQMDRGSRL
                     YAQYCFLCHQSNGRGVPSAFPPLAKSDFLAADKERSIRILITGLNGAITVNSNVFNGV
                     MPPAPYDDKQLADVLTFVHNSFGNSNGPVTFEQVKKIHDQLGGGQIVADPYPYAPLPR
                     SPEGFTLREVVRMPNHPTRMASDGQGKVLYVLCENADVWRVDIANGALQRILAGEDYA
                     SAQGGHASCVGLMLDAQKRLYVVANYLRDVQPYVMNEVTIYRTTALRDGEPAEPRPWL
                     QTNYPWGVGPFNHSVGNIGQGPDGFLYVTSGSRTDAGEPGGDPHYSQAGETPITATIW
                     RLDPRAEKPKIEIFANGVRNPYGFCWNDKGEMFATENGPNADAPEELNQIKRGGHYGF
                     PYQFSNWTKKAYDYQPDPPPGLKMTIPIANFGPDAGGSADKPLYSFQP"
     gene            complement(1713..4649)
                     /locus_tag="VG754_13035"
     CDS             complement(1713..4649)
                     /locus_tag="VG754_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWF20188.1"
                     /translation="MGADSSTNHGSGPNSIFEPVHYRPLAAKVMGLKNNTVSLIGAWR
                     IDAKPGQGIREKPLNAANWGNFHVPGQWTHQGYDVPLDKTAALAKEFIIPAEWAGYRI
                     FLRLDAVHGGTHYWLNGKPLGYSENLFTPVEWEITDVAKLGQTNRLDLEMKVDTASER
                     LSYSSDYAACSLGGVDRAVRIYALPRLHISTMRLDAGLDKDYRDGELQIAFDLDNPDS
                     DVESGLDVSLQLFDATGKSVEHSNPKIALDPLKPGLNPVNIKSRVAHPLKWNAEQPNL
                     YRLVIKLTRNGKLQEQIERNIGFRKIEVIDRQLYVNGARVKLAGVCHHEIDPLTGRSD
                     TMRHAEEDVKLFKSANLNDVRTSHYPPTQEFLDAADRYGLYVESEAPLCWLSPTKELT
                     NLNAVLTPTSAMIDYNQSHPSIIIWSLANESHWSVLFEESHKLCRQLDPTRPTEINHT
                     FTSEDKVTCEIMNRHYQDLPYDEILKDDPRPFMHGECFFLVYHERTDVDIDPGLRELW
                     ASGSAEPTSNWGMACIANLKAHTGLQPGIFPGAWSYIYNSSHCIGSELWAGIDDITYL
                     PGGKAISGEHGNAKWGLIDGWRRRKPELELAKFVFSPIWFPVRTLDYKPGQASMRVPV
                     ENRYSFTDLSQLDFIWELNGAKGKVHTSAPPEAKTEIEIPVRKGTPEGATLLLRVVKG
                     RNEIVSATFSLGERKPSPLPEPQAGAPKWNDDGKLITIEGNGFSLVLDRTTGNFDVAN
                     PRHHAPIATFPSLHVTRHDFGDLETKKPQYAELPDAKTRVVESVTVAEVNHGLELTVK
                     DHYDHFAGTVRWLMDKDGVGRISYDYVYSGDDLDSREIGIKALLPAACDEIKWRRWSE
                     WGSFPKDCICRTEGTAMARRDKKWPEQPANVKPVWPWSQDQTELGTADFRSIKFCIYE
                     ASLVAPNGSGVRVDANASAHFRACLAEQGVNMYILSQCPLAEVVLKKGARLRGEFSVR
                     LMAKAR"
     gene            complement(5012..>5354)
                     /locus_tag="VG754_13040"
     CDS             complement(5012..>5354)
                     /locus_tag="VG754_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20189.1"
                     /translation="MASTPGNGYWLVGSDGGIFSFSANFYGSMGGQHLNAPIVGMSAN
                     PGGTGYWLVGSDGGIFSFNVNYYGSMGGHQLAQPVCGMQSSVDGAGYWLVAKDGGIFS
                     FGDSPYKGNAN"
BASE COUNT         1143 a         1513 c         1488 g         1210 t
ORIGIN      
        1 ggctggaaac tgtaaagcgg tttgtccgcg gaaccgccgg cgtccgggcc gaaattggcg
       61 atggggattg tcatcttcaa tccgggcggg ggatcgggct gataatcata cgcttttttg
      121 gtccagtttg agaattgata cgggaaaccg taatgtccgc cgcgtttgat ttggttgagt
      181 tcctcgggcg catcggcgtt cggaccattt tcggtcgcga acatttcgcc cttgtcattc
      241 cagcaaaagc catacggatt gcgcacgccg tttgcgaaga tttcaatttt cggtttttcc
      301 gcgcgcggat cgagccgcca gatcgtggcc gtgatgggag tttctccggc ttgggaataa
      361 tgcgggtcgc cgcccggttc gccggcgtcc gttcgcgagc cgctggtgac atagagaaaa
      421 ccatccggcc cctgcccgat gttgccaacg ctgtgattga acggcccaac gccccaggga
      481 taattcgttt gcaaccatgg acgcggctcg gcgggttcgc cgtcgcgcaa cgccgttgtg
      541 cggtaaatgg tcacttcatt catcacgtaa ggctgcacgt cgcgcaaata attggcgacc
      601 acgtagagcc gcttttgcgc atcgagcatc aaaccgacgc aactggcgtg tccgccttgt
      661 gcggatgcgt aatcttcacc ggcaagaatc cgttgcaacg cgccgttcgc gatatcgacc
      721 cgccagacat cggcgttttc gcacaggaca taaagcacct tgccttgccc atcgctcgcc
      781 atgcgggtgg gatgattcgg catccggacc acttcgcgca acgtgaagcc ctccggcgag
      841 cgcggcaaag gcgcgtacgg ataagggtcc gcaacgattt gcccgccgcc gagctggtcg
      901 tgaatttttt tgacctgctc aaaagtgacc gggccgttgg aattgccgaa gctgttgtga
      961 acaaacgtga gcacgtccgc caattgcttg tcgtcatacg gcgcgggcgg catcacgcca
     1021 ttgaagacat tgctgttgac cgtgatggcg ccgttcaacc cggtgatgag gatgcggatg
     1081 cttcgttcct tgtcagcggc caagaagtcg gattttgcga gcggcggaaa agccgacggc
     1141 acaccccgcc cgttcgattg atggcacagg aaacaatact gcgcgtaaag ccggctgcca
     1201 cggtccatct gctccttgct gacgctttgg gtttcagaaa ccgacgcgaa caccgagccg
     1261 gtgagctgcc aaagaagcag gaggacagca acgaaacttc gatttgcggc gcgtaacata
     1321 ttgagtttta aggtgatgat tctgcacaat ttaccgggcg tcgcaaaggg aaagcgaact
     1381 tttcgggaca cttttttggg ttaacccgtc ccgctgctca cgatgagccc gctgcacgga
     1441 acccttccgg tccaagtctt gacgcaaggc ttccagccgt tgaaacccca cgcaattgat
     1501 gttcctttgc gccttgatct tctggcaagc ccccagtttc cgggccgagg cgcttttcgc
     1561 aggcttgggc ggcaacaccg catgcacaaa gcggagtcgg caccattcgg gcgctgccac
     1621 cgccttgggg cagcgccgcg gaaatttgca aggcggctgg aaagtcgcct cccttttgca
     1681 tactgtttct tctatcgaag gcttggacga ccttaccgcg cttttgccat caagcggacg
     1741 ctaaattcgc ccctgagccg cgcgcctttc ttcagcacga cttcagccag cgggcattgg
     1801 gacagtatat acatattgac gccttgttca gccagacatg cgcggaagtg ggcactggca
     1861 ttggcatcaa cccgcacgcc agaaccattg ggagctacga gtgatgcctc atagatacag
     1921 aacttgattg acctgaagtc ggccgtccca agctccgttt ggtcctgtga ccacggccag
     1981 acgggtttca cgttcgcggg ttgctccggc cattttttgt cccgacgggc catggccgtg
     2041 ccttcggtcc ggcagatgca gtctttcgga aagcttcccc actcggacca gcgccgccat
     2101 ttgatttcgt cgcaggcggc gggaaggagg gccttgatgc ctatctcccg cgaatcgagg
     2161 tcgtcgcccg agtaaacata atcgtagctg atcctgccca cgccatcttt gtccatgagc
     2221 cagcggacgg ttccggcgaa atggtcgtaa tgatctttca cggtcagttc caatccatga
     2281 ttgacctccg ccaccgtgac gctctcgacg acccgcgttt tggcgtcggg caactcggcg
     2341 tattgcggtt tcttggtttc caaatcgcca aagtcgtgcc gggtcacatg cagggaaggg
     2401 aacgtcgcaa tcggcgcatg atgcctggga ttggcgacgt cgaaattgcc cgtcgtccgg
     2461 tcaagaacca gcgagaagcc gttgccttct atggtgatca actttccatc atcgttccac
     2521 ttgggcgcac ccgcttgcgg ctcaggcaaa ggagaaggct tccgttcgcc aagcgagaac
     2581 gtggcgctga cgatttcatt gcggccttta acgacacgca atagcagcgt ggcgccttcg
     2641 ggcgttcctt tccgaacggg aatttcgatt tccgttttcg cttccggggg agcgctggtg
     2701 tgaaccttgc ccttcgcgcc gttcaactcc cagatgaagt cgagctggct taggtccgtg
     2761 aatgagtaac ggttttccac gggaacgcgc atcgatgcct ggccgggttt gtaatcaagc
     2821 gtgcgaaccg ggaaccagat cggcgagaac acgaacttgg caagttccag ttcaggcttc
     2881 cgccgccgcc atccgtcaat caaaccccat ttggcattgc cgtgctcgcc gctgatcgcc
     2941 ttccctcccg gaaggtaagt gatgtcatcg atgcccgccc ataactcact gccgatgcag
     3001 tggctggagt tatagatgta actccatgcg cctgggaaaa tgcccggttg caagccggta
     3061 tgcgccttca aattcgcgat gcaggccatg ccccaattcg aagtcggttc ggcgcttccg
     3121 ctggcccaca actcgcgcaa accgggatca atgtccacgt cggtgcgctc gtggtaaaca
     3181 aggaagaagc attctccatg catgaatggc cgcggatcat ccttcaaaat ctcgtcgtat
     3241 ggcaagtcct ggtaatggcg gttcatgatc tcgcatgtga ctttatcttc gctggtgaaa
     3301 gtatggttga tctcggtcgg ccgggtgggg tccagttgcc tgcacagctt gtgtgattcc
     3361 tcgaacagga cgctccaatg agattcattc gcaagcgacc atatgatgat gctgggatgc
     3421 gattgattat aatcaatcat cgcggaagtg ggtgtgagaa cggcgttgag gttggtcaat
     3481 tctttcgtcg gcgaaagcca gcagagcggc gcctccgact cgacatataa cccgtaacgg
     3541 tctgccgcat ccaaaaattc ctgcgtgggc gggtagtgcg atgttcggac atcgttaaga
     3601 ttggcgctct tgaaaagttt aacgtcttcc tccgcatggc gcattgtatc cgagcgtccg
     3661 gtcaacggat caatttcgtg atggcaaacg ccggcaagct tcacgcgcgc gccgttgaca
     3721 taaagctgcc ggtctatgac ctcgattttg cgaaaaccga tgttgcgctc gatctgctcc
     3781 tgtaactttc cgttccgggt cagttttatc acgagccggt agaggttcgg ttgttcggcg
     3841 ttccacttca acggatgcgc gacacgcgat ttgatattca ccgggttcaa tcccggcttc
     3901 aaaggatcaa gggcgatttt cggattcgag tgctccaccg attttcccgt cgcgtcgaaa
     3961 agttgcaagg aaacatcgag gccgctttcg acatcagaat ccgggttatc gaggtcgaaa
     4021 gcgatttgca attcgccgtc ccgatagtcc ttgtccaaac ccgcatccaa gcgcatcgtg
     4081 gaaatatgga gccgcggcag agcgtaaatc ctcacggcgc ggtccacgcc tcccaagctg
     4141 cacgcagcat agtcgctgga gtaggaaagc cgttccgaag ccgtatcgac cttcatttcg
     4201 agatcgagcc ggtttgtctg cccgagtttt gcgacgtcgg ttatttccca ttcgaccggg
     4261 gtgaacaagt tctcgctgta gccgagtggc ttgccattca accaataatg tgtcccgccg
     4321 tgaacggcgt ccaaacgcag gaatattcta tatccagccc attccgcagg gatgataaac
     4381 tctttggcca gcgcggcagt cttgtccaat ggcacgtcat atccctgatg cgtccattgg
     4441 ccggggacgt ggaaattgcc ccagtttgcg gcattcaaag gcttctctct gataccttgt
     4501 cccggctttg catcaatccg ccacgcacca atgagtgaaa cagtattgtt ttttaatccc
     4561 atcactttcg cagcgagggg acggtaatgc accggctcga aaatcgaatt cgggccgctc
     4621 ccatggttgg tgctggaatc agcgcccact gccaagacag ttccgaacga gatgatacaa
     4681 aacatcagaa cacgcatggc taaaaatttc attcggtttc gtctaattgt gaacggctgc
     4741 gcgccagtta atcaattggt ttggcgatat ttatgcgtgc attgtttcag gaaaggcaag
     4801 aaacggaatt aacaaatcga ttatcaaatt taacagcaag atgacagcaa ttttccacga
     4861 agtcgctctc gcggaactga agaaagggaa ttaggaaatc aggaaatcag gaaacgactg
     4921 tgtcccttaa cgcgcgcaac gacaagtgcc agaataaaat aaaaagcggg aggcccgaaa
     4981 gcctcccgcc gcgagccctt cctcacccca cttaattggc gttgcctttg taaggcgagt
     5041 cgccaaatga gaagatgccg ccgtccttcg ccaccagcca gtaacccgcg ccgtccacgg
     5101 aagattgcat gccgcagacc ggttgggcca actgatgtcc gcccatggag ccatagtaat
     5161 tgacgttgaa tgagaaaatg ccgccgtccg agccgaccag ccagtagccg gtgccgccgg
     5221 gatttgcgga cattccaaca atcggagcgt tcaaatgctg accacccatc gagccgtaaa
     5281 agtttgcgct gaagctgaag atcccgccgt ccgagccgac gagccagtaa ccgttgcccg
     5341 gcgttgaagc catg
//
LOCUS       DASZMM010000544         9425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2981493, whole genome shotgun sequence.
ACCESSION   DASZMM010000544 DASZMM010000000
VERSION     DASZMM010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2981493"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VG754_13045"
     CDS             <1..230
                     /locus_tag="VG754_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20190.1"
                     /translation="IIQPLSFTSFGTTANNVFQMQLSGAVAGSNYVLQATTNFITWTP
                     LNTNLAGSNTFNLIDSAASNYPYRFYRILQQ"
     gene            267..845
                     /locus_tag="VG754_13050"
     CDS             267..845
                     /locus_tag="VG754_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20191.1"
                     /translation="MMVAKIAVKLALVIVFVALSASAQSNQVVATNREDPHEQTIAWA
                     LKVEEIRTNCIQNRRRICGKILKVLPEGLVIDSGYTNLARTQINRSWLIPGTVAAERA
                     TNVIEEVHPGAFCIGLVFLTDLPKSPGAKPKLYDYVNLEAFPAGHYTYVSVGDLHRTV
                     RKFSTKLPMAVQYALDHDPLNARPSSNNSAHP"
     gene            967..1593
                     /locus_tag="VG754_13055"
     CDS             967..1593
                     /locus_tag="VG754_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWF20192.1"
                     /translation="MVWCMGRVSDTKAKLMQVVVELIWIGSYGSTTIDDICEKAQVKR
                     GSFYYFFESKADLAVAAVDEEWQKHRPELDAIFSPVVPPLERLRRYCEYGYKIQAEWK
                     EKCGHVLGCALFTLGCEVSTQEPQLTKKIQQILDQKKKYLESAIRDAHAAGLLHAPDA
                     AAKARILFTYYEGQLTEARIQNNLDILLEAIRGTYELLGIQESLPATK"
     gene            1644..1943
                     /locus_tag="VG754_13060"
     CDS             1644..1943
                     /locus_tag="VG754_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20193.1"
                     /translation="MKNNNNKLKGSVSEACAKYCQKILSQVAKTKNAVLAQFRDLVAD
                     NEQLLQLALNEAEALAWQTRYPQLVFQDLAEEKAKDVVSWVAHQREVRSGINFRN"
     gene            2009..3184
                     /locus_tag="VG754_13065"
     CDS             2009..3184
                     /locus_tag="VG754_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF20194.1"
                     /translation="MKKTIFLSGTTVLALAGIFLSAGCSQKPSQSSAPPAPAVTVTTI
                     SQKDIVEWEEFTGRTEAMESVEVRPRVSGYIREVRFQSGQLVKKGDVLFVIDPRWHES
                     DYNRLQAEAGRARAQLDNAKREADRTSNLLKDHAISAEESEARISRYEEAKAALDAAE
                     AARDYAKLDLEFTQVRAPIDGRVSRALITQGNYVTGGSGAGTVLTTLVSVDPVYVYAD
                     VDEDSLLRFNALVAAGRIETNADGRTPVQLQLADEQGFPHQGCIESFDNHLDENTGSI
                     LLRAVFPNADGRIVPGLFARIRLPMSEKHPAVLVTESAVGTDQARKYVLTLTSTNTVA
                     YQPVELGPEFEGKRVVRSGLTNGDKVVVNGLQRVRPGMAVTPQDQDGAETGRMAYGK"
     gene            3206..6343
                     /locus_tag="VG754_13070"
                     /pseudo
     CDS             3206..6343
                     /locus_tag="VG754_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
     gene            6431..7828
                     /locus_tag="VG754_13075"
     CDS             6431..7828
                     /locus_tag="VG754_13075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWF20195.1"
                     /translation="MKVGPDYHQPSNAVPSEYKSAELGRWKEGAPLDTVPKGVWWEVF
                     GDAELNRLEAHAQQANGDLKAAIARVDQARASARVARADLLPSLNLEPSLNRQRYSPN
                     QNPSFGAITANTFSVPLDLSYEVDLWGRVRRGFESARADAQSSLANFYNVLLTLQSDV
                     AQNYFALRSLDAEMAIVNGTVDLRHEQVRLVRSRFNGGVGNELDVARAETELAITEAE
                     AASVAQRRAQLENAIAILVGVNPASFHLAPENTANWKPEPPVIPAGLPSDLLERRPDV
                     AAAERQLASANARIGVAKASFFPVITLTGSGGFLSADVDSLFNWSSRTWSIGPSVSLP
                     IFAGGRLRANYHRSQAAFAEAAGQYQQQVLVAFGDVEDSLSDIHYLADQAAAQERAVS
                     HSKRASDLATERYTSGIVSYLEVVDASRDLLQSERSAAQLAGQRLIADVQLIKSLGGG
                     WSETALFAQAKNPNK"
     gene            complement(7838..7914)
                     /locus_tag="VG754_13080"
     tRNA            complement(7838..7914)
                     /locus_tag="VG754_13080"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7878..7880),aa:Asp,seq:gtc)
     gene            complement(8151..>9425)
                     /locus_tag="VG754_13085"
     CDS             complement(8151..>9425)
                     /locus_tag="VG754_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF20196.1"
                     /translation="FATEFADQKKRVGLIGCGWYGKTDLLRLIQVAPVEVVSLCDVDK
                     NMLAGAADIVASRQLSKKKPRTYGDYREMLKEKDLDVVLVGTPDHWHALAMIAALESG
                     ADIYVQKPISVDVIEGQAMLAAARKHNRVVQVGMQRRSTEHLIEARDRFVKTGKLGKV
                     GLVELCCYYHKSETENPPDSAPPDYLDYEMWTGPAPMRPYNPMVHPRKWRQFYEYGNG
                     TVGDMCIHMLDTARWMLELGAPKTVSSTGGIIMSKASKADITDTQTATFDFGDVQMIW
                     QHRTWGSSPDPKYPWAAIIYGDQGTLKLSVYGYDFIPANGEPIHRDVTYELEQFPEDK
                     TEKDLEKHVAPAVRGHMKNFLSCIASRGRPVSDIEEGYISSTACILANISMRLGGRAL
                     KWDETTGRIIGDDEAKQMLRRPYRAPWVHPEA"
BASE COUNT         2082 a         2580 c         2607 g         2156 t
ORIGIN      
        1 tcatcattca gccgctttca ttcacctcgt ttggcaccac tgccaacaat gtcttccaga
       61 tgcaattgtc aggcgcggtt gcaggcagca attatgtgtt gcaggcgacg accaacttca
      121 taacttggac ccctcttaac accaatcttg ctggcagcaa cacgtttaat ctgattgatt
      181 ctgctgcgag caattatcca tatcgcttct atcggatcct acagcaatag atgtcatcgc
      241 agttgatttg ttgatgaccc atgcgcatga tggttgcaaa aattgctgtg aagcttgcgc
      301 tcgtcatcgt ttttgttgcc ctgtccgcct ctgcccagtc gaaccaagtt gtggcgacga
      361 accgtgagga cccccatgag caaaccatcg catgggcgct gaaagtcgag gaaatccgga
      421 cgaattgcat tcagaaccgc cgccgtatct gcggcaagat tttgaaggtc ttgcctgaag
      481 gacttgttat tgacagcggt tataccaatc ttgcgcgcac ccaaatcaat cgttcatggc
      541 tcattcccgg aacagtggca gcagagcgcg ctaccaatgt catcgaggaa gttcacccag
      601 gcgctttttg catagggctg gtttttctga ccgacctgcc gaaatcgccg ggtgccaaac
      661 ccaaactgta cgattacgtc aatctcgagg cctttcccgc cgggcattac acttacgtct
      721 ccgtcggcga ccttcaccgg accgtgcgaa aattttcaac caaacttcct atggcagtcc
      781 aatatgcgct tgaccatgat ccactgaacg ctcggccaag ttcgaacaat tccgctcatc
      841 cttgaaacgg tttcggcgac tttgctgaga tttttgcaca agctcatacc agctcctaca
      901 ggtgaatggg ttctccaagc agaaaaataa ttctaaaaaa tagttgacta gtttgtcaac
      961 tttgatatgg tgtggtgtat gggacgcgta agcgatacga aagcgaagtt gatgcaggtg
     1021 gtggtggagt tgatttggat cggctcttac ggcagcacca cgattgatga catttgcgag
     1081 aaagcgcagg tgaaaagggg cagtttctat tatttcttcg agtcgaaggc cgatttggct
     1141 gtggctgcag ttgacgaaga atggcaaaag catcgccccg aactcgatgc catcttttcc
     1201 ccggttgtgc ccccattgga acggcttaga cggtattgcg aatacggtta caaaatccag
     1261 gcggaatgga aggaaaaatg cggacatgtg cttggttgtg cgctatttac gctcggatgc
     1321 gaggtcagca cccaagagcc gcaattaacg aagaagattc agcaaatttt ggatcagaag
     1381 aaaaaatatc tcgagtcagc cattcgggac gcccatgccg caggattgct tcacgccccg
     1441 gatgcagccg ccaaggctcg catacttttc acctattatg aaggccaatt gaccgaagct
     1501 cggatccaaa acaacctgga tattctcctt gaggcaattc gtggaacata cgaattgctg
     1561 ggaatccaag aatctcttcc cgcaaccaaa taaatttttt taaaaacaaa attgactaac
     1621 ttgacaacta tcaaggaaat cacatgaaaa acaacaacaa caaactcaaa ggttcggttt
     1681 cagaagcctg cgccaaatat tgtcagaaaa tactgtcgca ggttgcaaag acgaaaaacg
     1741 ccgtcctggc gcaattccgg gatctcgttg ccgataatga acaattgctt caattggcct
     1801 tgaacgaggc tgaagccctc gcgtggcaaa ctcgctatcc gcaacttgtc tttcaggact
     1861 tggctgaaga aaaagccaaa gatgtggtaa gctgggtcgc gcaccagcgc gaggtgcgtt
     1921 ccggcatcaa ttttcggaat tgatgtccaa aaagggcttg cccattcgtt gcactacgga
     1981 aacgtcacac tatcgaaagt ttcatgtcat gaagaaaacg atttttttga gcggaacaac
     2041 cgtccttgcg ctggcaggaa tatttttatc agcaggatgc agtcaaaagc cgtcgcaatc
     2101 atccgcgcct cccgcgcccg ccgtgacggt cacgaccatc agtcaaaagg acatcgtgga
     2161 atgggaagag ttcaccgggc ggaccgaagc gatggaatcg gttgaagtgc gtccgcgagt
     2221 ttcgggctac atccgcgagg tccggttcca atcgggacag ttggtgaaaa agggcgacgt
     2281 gctattcgtg attgatccgc gctggcatga atcggattac aaccgcttgc aagccgaagc
     2341 ggggcgcgcg cgcgcgcagt tggacaacgc caaacgcgaa gcggaccgca cctcgaacct
     2401 gctgaaagac catgccattt ccgccgagga aagcgaggcc cgcatctccc gttacgaaga
     2461 ggcgaaggcg gcgctggacg cagccgaagc cgcgcgcgat tacgccaaac tcgatctgga
     2521 attcacccag gttcgcgcgc cgattgacgg gcgtgtcagc cgcgcattga tcacgcaagg
     2581 caattacgtc accggcggct ccggcgcagg cacggtgttg accacccttg tctcggttga
     2641 tcccgtgtat gtatatgcgg atgttgacga ggattcactc ctgagattca acgcattggt
     2701 tgcagcaggc agaatcgaga cgaatgccga tggccgcacg ccggtgcaac tgcaattggc
     2761 cgacgaacaa ggattcccgc atcaaggttg catcgaatcc ttcgacaatc atttggacga
     2821 aaacacggga agcattcttt tgcgcgccgt atttcccaat gctgacggac gcatcgtccc
     2881 cggattgttc gcgcggatcc gtttgccgat gagcgaaaag catcccgcgg tgctcgtcac
     2941 cgagagcgct gttggcaccg atcaagcgcg aaaatatgtt ttgacgctga cttcgacgaa
     3001 caccgttgcc tatcagccag tggaacttgg gccggaattt gaaggcaaac gagtcgttcg
     3061 ttccgggctt accaatggcg acaaggtggt cgtgaatgga ttgcaacgcg tccgtcccgg
     3121 aatggcagtc acgccgcagg accaagacgg ggctgaaaca ggccggatgg catacggcaa
     3181 ataaaaccct caaccgggaa ccaccatgaa cttctcgaat tttttcattc gccggcccat
     3241 ttttgccggg gtcctgtcaa cgatcatttt cctggtcggc ttgctcgccc tttggcggct
     3301 gccgatcagt gaatatccgg aagtcgtgcc gccgacagtg gttgttcgcg ccgaatatcc
     3361 cggcgcgaat cccaagacga ttgcggaaac agtcgcctcg ccattggaac aatccatcaa
     3421 tggcgtcgaa gactcgctct atatgttttc gcaagccacg cccgacggcg tcatgaatct
     3481 cacggtcacg tttaaactgg gaacggacgt tgacaaggcg caggtgcagg tgcaaaaccg
     3541 cgtcgctcaa gccttgccga aattaccgga agaagtccgc aacctgggcg tcaccaccac
     3601 caaacaatcg tccgacttga cgatggtcgt ccatttattt tccccgaacg gaaggtatga
     3661 cgaagtttat atgcgcaatt acgccacgtt gcaggtgaag gacgtgctcg cgcgcattcc
     3721 cggggccggc agcgtgcagg tgttcggttc cggcgattat gcgatgcgcg tctggctgaa
     3781 gccggacaaa atcgccgagc gcaacctgac ggcgagcgat attgtcgcgg ccattcgcga
     3841 acaaaacgtg cagattgccg ccggtgcggt cggccagcag ccgttgaagc acccggttga
     3901 tttcgaattg caaatcaatg ccaagggccg tctcgtttca cccgaggagt tcggacaaat
     3961 cattgtcaaa gtcggtcccc aaggccagaa gacgctgctc aaggacgtgg cccgaattga
     4021 gttgagcgcc gacagttatt ctttgcgctc gctcttgaac aacaaaaccg ccgtggcatt
     4081 gcccatcttt caacttccag gcgccaatgc cctccagctt tccacggacg tccgcaataa
     4141 gatggaagag ttgaagaaaa attttccgga cgggattgat tattcggttg tttacgaccc
     4201 gacagtgttt gtgcgccatt ccatcgaggc ggtgatccac acgttggtgg aggcgatcct
     4261 gttggtggtg attgtcgtca tcatcttcct gcaaacctgg cgcgcgtcaa tcatccctct
     4321 ggctgcggtg ccggtttcgc tcgtcggcac gttcgcggtg atgctcgcag tcggtttttc
     4381 gatcaacaac ctttcactct ttggcctggt gctcgccatc ggcatcgtcg tggacgatgc
     4441 catcgtggtg gtcgaaaacg tggaacgcaa catcgcgctt ggccttgagc cgcacgcagc
     4501 cgccaaaaag gcgatgagtg aagtgaccag cccgatcatc gccacagcgc tcgtgctctg
     4561 cgcggtgttc gtgccgaccg cattcatcag cggcttgacc gggcaattct acaagcagtt
     4621 cgccatcaca atcgcgatct ccacagtcat atctgcattc aattcgctga ctctctcccc
     4681 tgccttgtgc gccgttttgc tcaagaggca cgacgaatcc aaggattggt ttgcgcgcgg
     4741 catggacatc gccttcggct ggttcttccg ccctttcaac cgtgcatttg cctgggccgg
     4801 aaacaaatat tccgtcggcg tttcttccgt cctgcgcaaa actgccgccg cgcttgtcgt
     4861 atatgccggg ttggtgtttt tgacaggctg gagcttcaac aaagtcccga ccgggttcgt
     4921 tcccactcag gacaaacaat atctggtcgc gttcgcgcaa ctgccggacg cggcgtcgct
     4981 tgaccgcacg gaagcagtaa tccggcgcat gtcggacctt ggcctgaaaa cgccgggtgt
     5041 gcaaagcgcc gtggccttcc ccggattgag catcagtggt ttttcagttg cgcccaatgc
     5101 cggcatcgtt tttttctgct tggacccgtt tgaggaccgc aaatcaaaag acctcagcgg
     5161 cccggcaatc gcagacgctt tgaataaaaa attcagttca atccaggacg ccttcgtgct
     5221 gacagtgatg ccgccgccgg tgatgggctt gggaacaatc ggcgggttca aattatatgt
     5281 cgaggaccgc gccgatcttg gttacgacgc gctttatcaa accatccagt caactgttaa
     5341 cgaaagctcc aagaagccgg ggctcgacag tgtgttctcc acgttcactg tgaatgttcc
     5401 gcaattggac gcggacattg accgcatcaa agccaaggaa gaaggcgtgc cattgcaaaa
     5461 cttgttcgag acgatgcaga tttatctcgg ctcgctttac atcaacgatt tcaaccggtt
     5521 tggccgcacg tttgaggtga ttgcgcaagc ggacgcttcg tatcgcgaaa aggcggcgga
     5581 catcacccgc ttgaaaacgc gcaatgaccg cggacagatg gtgccgttgg gcgctgtggt
     5641 caaggtgacg gaaactcacg gtcctgatcg cgccgagcgt tacaatggtt atccggcagc
     5701 cgaaatcaat ggcgcgccag cggccggtta cagttccggg caggccgaag cgatgattga
     5761 taaaatcgcg gcgaacaaca tgcccaaagg gatgaactac gaatggaccg agctgaccta
     5821 ccagcgcatc cttgcgggca ataccgccgt ctatgtatat ccgttgtgca tcctgctggt
     5881 gttccttgtg ctggccgcgc tttatgaaag ttttcgcctg ccgctcgcca tcattctcat
     5941 tgttcccatg tgcctgcttt ttgccatcgc cggagtttgg ctcaaggggg gtgacaataa
     6001 tattttcaca caaattggtt tgatcgttct tgtcggactg gcttgcaaaa acgccatttt
     6061 gatcgtcgag ttcgccaagg acaaacatga acagggcgtt ccgccgattg aagccgccat
     6121 tgaagcgtgc cgcttgcgtt tgcgcccgat tttgatgact tcaatcgcgt tcatcgcagg
     6181 cgtgtttccg ctggttgtcg cgcacggcgc gggcgctgaa atgcgccagg caatgggcgt
     6241 ggctgtgttt gcgggaatga taggcgtaac gctttttggc ctatttttga cgcctgtgtt
     6301 ttacgtgacg ttgatgaagc tcggtttcaa gaaagctcct gtccatgcgc cccaaacggt
     6361 tggcgcaact gtcggtgtta ttgttgcgct tgcgttgttg gcgagcgcgt caactgcttc
     6421 agccggatgg ttgaaagttg ggcctgatta tcaccaaccg agcaatgcgg ttccttcaga
     6481 atacaagtcc gcggaacttg ggcgctggaa ggaaggcgcg ccgctggaca ctgtgccgaa
     6541 gggcgtctgg tgggaagttt tcggcgatgc cgagttgaac cggctggaag cgcatgcgca
     6601 acaagccaac ggcgacttga aagcagccat cgcgcgtgtg gaccaagccc gtgcctctgc
     6661 gcgagtcgcg cgcgccgatc tgctgccgag tttgaacttg gagcccagtt tgaaccgtca
     6721 gcgctattca ccgaaccaga atcccagctt cggcgcaatc accgccaaca cgttttctgt
     6781 cccgctcgat ttgagttatg aagtggattt gtggggacgc gtgcgccgcg gtttcgagag
     6841 cgcccgcgca gatgcgcaat cttcgctggc caatttttat aatgtgctgt tgaccttgca
     6901 atccgatgtt gcgcaaaatt attttgcgct gcgctcactg gatgccgaga tggcgatcgt
     6961 caatggcacc gttgatctgc gacacgaaca ggtgcgtctc gtgcgcagcc gtttcaacgg
     7021 cggcgtgggc aatgaactcg atgtggcgcg cgcagaaacg gaattggcga tcaccgaggc
     7081 cgaggccgcc tcggttgccc aacgccgtgc tcaactggaa aatgccatcg cgattttggt
     7141 gggcgtcaat ccggcgtcgt ttcatcttgc gcctgaaaac acggcgaact ggaagccgga
     7201 gccgcctgtg atccccgccg gtttgccttc cgatttgctt gaacgccgtc cggatgttgc
     7261 cgctgccgaa cgtcaactcg cttccgcaaa tgcccgcatc ggcgttgcca aggcgtcatt
     7321 ttttccggtc atcacactga caggttcggg tggttttcta agcgcggatg ttgattcgct
     7381 tttcaactgg agcagccgca cgtggtccat cgggccgagc gtttcgctcc cgatttttgc
     7441 cggcggaaga ttgcgcgcga attatcatcg ttcgcaagcg gcgttcgctg aagcggcagg
     7501 ccaatatcag cagcaggttt tggtcgcatt cggcgatgtg gaagacagct tgtccgacat
     7561 ccattatctc gcggaccaag cagcggcgca ggagcgcgcc gtcagccatt ccaaacgcgc
     7621 gtcggacctc gccaccgagc gttacacttc gggcatcgtg agttatctgg aagtggttga
     7681 tgcgagtcgc gatcttttgc aatcagaacg ttctgccgcg caattggccg gacaacgctt
     7741 gattgctgac gttcaactca tcaaatccct cggcggcggc tggagcgaga cggcattatt
     7801 cgcccaagcc aaaaacccca ataaataaca gggaaaatgg cgggagtggc ggggctcgaa
     7861 cccgtaacct ctgccgtgac agggcagcgc tctaaccaat tgagctacac ccccactaag
     7921 gggaggcgaa acttacgaaa tacactcttt cgcgtcaagc tgaagcgaag aaaattcggg
     7981 cagcagacgc caaggaggtg ggggtgggga accccgctga agaaacgtgc tgctacccga
     8041 acacaataat tttatgaccc agaaccgatt tgtcaactct tatacacaac cggttgtatg
     8101 ttcgtttgca tgaaacacga ttccatgcat tcccgcgcct ttcccttcaa ttatgcttct
     8161 ggatgcaccc agggcgcgcg atagggtcgt cgcagcatct gcttcgcttc atcgtcgccg
     8221 atgatgcgac cggtggtttc gtcccacttg agcgcgcgac cgccaagtcg catcgaaata
     8281 tttgcaagga tgcaggcggt gctggaaata taaccttcct cgatgtcgga cacgggacgt
     8341 ccgcgagaag cgatgcagga caaaaagttt ttcatgtgcc cgcgaactgc cggcgcgaca
     8401 tgcttttcca agtccttctc ggttttgtcc tcgggaaatt gttcaagttc gtaagtcacg
     8461 tcacgatgga tgggttcgcc attggccgga atgaaatcgt agccgtaaac acttagtttg
     8521 agcgtgcctt gatcgccgta aataatcgcg gcccacggat attttggatc aggtgaactg
     8581 ccccacgtgc gatgctgcca aatcatttgc acgtcgccga agtcgaatgt cgcggtctgc
     8641 gtgtcggtga tgtcggcctt gcttgccttg ctcatgatga tgccgccggt ggaactgacg
     8701 gtcttgggcg cgcccaattc gagcatccaa cgcgcggtgt cgagcatgtg gatgcacatg
     8761 tcgccgacgg tgccgttgcc gtattcgtaa aattgccgcc atttgcgcgg gtggaccatt
     8821 ggattgtaag gacgcatcgg cgcggggccg gtccacattt cgtaatcgag ataatcgggc
     8881 ggcgcgctgt cgggcggatt ttcggtttcg cttttgtgat aataacagca caactcgacg
     8941 agaccgactt tgccgagttt gccggtcttg acgaacctgt cgcgcgcttc aatcaaatgc
     9001 tcggtgctgc ggcgttgcat cccgacctgc acgacgcgat tgtgcttgcg cgccgccgcg
     9061 agcatcgcct ggccttcgat gacatccacg ctgatcggtt tttgcacgta aatatccgcg
     9121 ccggattcga gggcggcgat catcgccagc gcgtgccaat ggtcgggcgt gccgacgagc
     9181 acgacgtcca aatctttttc cttgagcatc tcgcggtaat cgccgtaggt gcgcggtttt
     9241 ttcttggaaa gttgccgcga cgcgacgatg tccgccgcgc cagcaagcat gtttttgtcc
     9301 acgtcgcaaa gcgagacgac ctcgaccggc gcgacttgga tgaggcgcag caaatcggtc
     9361 ttgccatacc agccgcaacc gatgagcccg acgcgttttt tctggtcggc gaattctgtc
     9421 gcaaa
//
LOCUS       DASZMM010000545         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2157771, whole genome shotgun sequence.
ACCESSION   DASZMM010000545 DASZMM010000000
VERSION     DASZMM010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157771"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VG754_13090"
     CDS             complement(<1..762)
                     /locus_tag="VG754_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF20197.1"
                     /translation="MLTTLRIKNLALVADLTLELQAGYNAITGETGAGKSIIIGALNL
                     VLGERADRSLIRSGSDSCSVEAVFEISKLRAPLKTFLEENGLEPSEENQLVLKRAFSS
                     SGTNRQFVNGSPTTLSALASLGEWLVDMHGPHDHQSLLHPSRQLAILDAFGNLQSRRE
                     NFAELVRRRNTVENEKSALIVDEKTYAQQMDLLRFQANEISAARLQPDEETAIQEEYR
                     RSSNAQKLLELTRAAQDLLSENEASLLTNAGLLGRA"
     gene            827..1621
                     /locus_tag="VG754_13095"
     CDS             827..1621
                     /locus_tag="VG754_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF20198.1"
                     /translation="MSFSFIFLGTGTSQGVPMIGSEYPPEFLANPKNHRTRPAIYVAS
                     EQAKIAVDTTPDFRTQILRENIRWLDAVVFTHSHADHIMGLDDCRRFCDLRGGVGLPI
                     YANAATMENLKRVYNYAFHEGPWPKGYFIPEPHLVEGPFRIGDLEITPLPLPHGRTTT
                     NGYLFVQDGKKRLAYMSDCKEVPVEVVKQIGGVEVAVLDALRRAPHPTHMCLDEALTA
                     ARRIGAERTYFTHLTHDYDHDLAQAELPPGVELAYDGLRIDYETRA"
     gene            1629..3326
                     /locus_tag="VG754_13100"
     CDS             1629..3326
                     /locus_tag="VG754_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HWF20199.1"
                     /translation="MKAFLILLLGVLGLTGGMNAFGAGRGDEVFVVYNSRVPESKGVA
                     EHYATVRQVPSRQVMGLDLPVCESISRADFREHLQKPLAKALESKGLIRYDAAIIPAT
                     NGEPRRVERRIVEAKIRYVLLCFGVPLIIAKDRDLHEPEASKLRVELQRNEAAVDNEL
                     ACLAVLDHMLLAGPRENPLFGSTNAYWFNPTNGIFMVTRLDGPTADIARSLVDKAMEA
                     ETNGLWGRGYFDMRGLTNGPFKAGDDWIRTAYEVCRLLGFDTVVDTNGGTFPAAFPLS
                     QVAFYAGWYDEHVSGPFARPTVEFMPGAFAYHLHSFSAATLRSTKHQWAGPLLDRGVT
                     ATMGCVDEPYLGGTPNIGIFYARFINNGFSYGEAAYACQNYLSWQTTVVGDPLYRPFG
                     RSPAELAEDLQRRHDKNLEWAYVRLANLKLLQGAPLERIIEALENADMTKGSAVLQEK
                     LADLYHTQGKPESSVHALQEVLNLNPTPQQRVRVMLTLSERLMASGRDEEAYQVYQQF
                     LKDCPDYPDPLFIYRSLLGLAEKLDHKSDAAKYQREITELNEPRIPSAKGPPQRHGT"
     gene            complement(3452..>3881)
                     /locus_tag="VG754_13105"
     CDS             complement(3452..>3881)
                     /locus_tag="VG754_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWF20200.1"
                     /translation="DAITHRPEFGFNVDPSQLGYQGVDYVRFIQKFGDRIYHVHMKDV
                     YWSSTPTEAGVFGGHTKFGDPRRFWEFRSLGRGSVNFERIIRALNEVNYQGPLSVEWE
                     DIGMDREHGATEAAAFVKKVDFAPSKVAFDAAFDKKAQAK"
BASE COUNT          837 a         1010 c         1150 g          884 t
ORIGIN      
        1 cgcgcgccca agcaagcctg cgttggttag cagcgaagcc tcgttttcgc tgagcaaatc
       61 ctgcgcggcc cgggtcagtt ccaaaagttt ttgcgcgttg ctggagcggc gatactcctc
      121 ctgaatggcg gtttcttcat ccggctgcag acgcgcggcg gaaatttcat tggcttgaaa
      181 gcgcagaagg tccatttgct gcgcgtaggt tttttcatcc acgatgagag cggatttttc
      241 gttttccacg gtgttacggc gtcggacgag ttcggcgaaa ttttcacggc gagattgcag
      301 gttgccgaaa gcatcgagga tggccagttg ccgcgaggga tgaaggagcg attgatggtc
      361 atgcggccca tgcatgtcca cgagccattc gccaagcgaa gcgagcgcgc tgagcgtcgt
      421 cggcgagccg ttgacgaact ggcggttggt gccggaactg gaaaaagcgc gcttgagcac
      481 aagctggttc tcttcactcg gttcaaggcc gttctcctcc agaaatgttt tcaacggtgc
      541 gcgcaattta gaaatttcaa aaaccgcctc gaccgagcaa ctgtcgctgc cgctgcggat
      601 gagcgagcgg tcggcgcgtt cgccgaggac gagattcaac gccccgatga tgatggactt
      661 gcccgcgccg gtttcgccag tgatggcgtt atagccggct tgcagctcga gggtgaggtc
      721 ggccaccaac gcgaggtttt tgatgcgaag tgtcgtcaac atgttaaact gtgcgcgcgc
      781 tcaatttgac gattatatcg aagttcggca aaataaaagt ttcgcaatgt cattttcgtt
      841 cattttcctt ggcacaggga cgtcgcaagg cgtgccgatg ataggctcgg aatatccccc
      901 ggaattcctg gccaatccga aaaatcaccg cacccgcccg gcgatttatg ttgccagcga
      961 acaggccaaa attgcagtgg ataccacccc cgatttccgc acgcagattt tgcgcgagaa
     1021 catccgctgg ctcgacgcgg tggtgttcac gcattcgcac gcggaccata tcatggggct
     1081 ggatgattgc cggcgttttt gcgatttgcg cgggggcgtc gggctgccga tctatgcgaa
     1141 cgcggcgacc atggaaaatt taaagcgcgt ctataattac gcattccatg aaggcccatg
     1201 gccgaagggt tattttatac ccgaaccgca cctggtcgag gggccgttcc gcatcggcga
     1261 cctggaaatc acgccacttc ccctgcccca tggccgcacg accaccaacg gttatctgtt
     1321 cgttcaggat ggaaaaaaac ggcttgctta catgagtgat tgcaaggaag tgcctgtcga
     1381 agtggtgaag caaatcggcg gtgtggaagt cgcggtgctg gatgctttgc ggcgcgcgcc
     1441 gcacccgacg cacatgtgcc tggatgaagc gttgacggca gcgcggcgca tcggcgcgga
     1501 acgaacgtat tttacgcatt tgacgcacga ttatgatcat gatttggcgc aggcggaatt
     1561 gccgcccggc gtggaactgg cgtatgacgg attgcggatt gattacgaga cacgggcgta
     1621 gttttgtgat gaaagcattt cttattctat tgctgggagt gttggggttg accggcggca
     1681 tgaacgcatt tggcgcgggt cgtggcgatg aagtgtttgt ggtttacaat tcgcgagttc
     1741 cagaatcgaa aggcgttgcg gaacattacg cgacagttcg ccaggtgccg tcccgacaag
     1801 tgatgggtct ggatttgccg gtctgcgagt cgataagccg cgcggatttt cgcgagcatt
     1861 tgcaaaagcc gctggcaaag gctttggaat cgaagggact gatccgttac gatgctgcga
     1921 tcattcccgc aacgaacggc gagccgcgcc gcgttgagcg ccggatcgtg gaagcaaaaa
     1981 ttcgttacgt gcttctttgt ttcggcgtgc cgttgatcat cgccaaggat cgggacttgc
     2041 acgaaccgga agcctcaaaa ttacgggtgg aattgcagcg caacgaagca gccgtggaca
     2101 acgaacttgc ttgtttggcg gtcctcgatc acatgctgct ggccggccct cgagaaaatc
     2161 cgcttttcgg ttccacgaac gcatattggt tcaacccgac caacggcatc ttcatggtga
     2221 cgcgccttga cggcccgacg gcggatatcg cgcgcagcct ggtggacaaa gcaatggaag
     2281 cggaaacgaa cggcctttgg ggccgcggct attttgatat gcgaggcttg accaacggcc
     2341 cgttcaaggc gggcgacgat tggatccgca ccgcgtacga agtctgccgc ctgcttggtt
     2401 ttgacacggt ggtggacacg aacggcggaa ctttcccggc ggcttttcca ctgagccagg
     2461 tggcgttcta tgcgggctgg tacgacgaac atgtttcggg gccgttcgcg cggccgaccg
     2521 ttgaattcat gccgggggcg tttgcgtatc atttgcattc gttcagcgcg gcgaccttgc
     2581 gctccacaaa acatcaatgg gccgggccgt tgctggatcg tggggtgaca gcgaccatgg
     2641 gttgcgtgga tgaaccgtat ttgggaggga cgccgaacat tggaattttt tatgcgcgtt
     2701 tcatcaacaa cgggttcagt tacggcgagg cggcttacgc gtgccaaaat tatctttcgt
     2761 ggcagacgac ggtggtgggc gatcctcttt atcgtccatt tggcaggtca cccgcagaat
     2821 tggcggaaga tctacagcgg cgtcacgaca aaaaccttga atgggcttac gttcggctgg
     2881 ccaatctgaa attgctgcaa ggggcgccgc tggaacggat catcgaggcg ttggagaacg
     2941 ctgatatgac aaagggaagc gccgtgttgc aggaaaagct ggctgatttg tatcacacgc
     3001 aaggcaaacc tgaatcatcg gtgcacgcgt tgcaggaagt cttgaacctc aatcccacgc
     3061 cgcaacaacg agtccgcgtg atgctcacgt tgagcgagcg attgatggcg tcggggcgcg
     3121 acgaagaagc ttaccaggtc tatcaacaat ttctgaagga ttgtcccgat tatccagacc
     3181 cgctgttcat ttatcgttca ctgcttggtc tcgctgaaaa gctcgatcac aaaagcgatg
     3241 ctgcgaaata ccagcgggaa atcaccgagt tgaacgagcc gcgcattcct tcggcgaaag
     3301 ggccacctca gagacatggc acatgaggtt tgttaaattg taacatggtt acatggttac
     3361 atggctatac cgttgcaaag gatcacctgt tgcatgtgac cgccctcttg taacggtgca
     3421 accttgtagc catgtaacaa attaaccatc ctcacttcgc ctgcgctttt ttatcgaatg
     3481 cggcatcaaa cgccactttg ctcggggcga aatcaacttt cttgacgaac gcagcggctt
     3541 cagttgcgcc atgttcgcga tccatgccga tgtcttccca ttcgacggaa agcggccctt
     3601 gataattgac ttcgttaagt gcgcggatga tgcgctcgaa attcaccgac ccgcgtccca
     3661 atgaacggaa ttcccagaat cggcgtgggt cgccgaattt tgtgtggccg ccaaacacac
     3721 ccgcttccgt tggcgtgctc gaccaataga catccttcat gtggacgtga tagatgcggt
     3781 ctccaaattt ctggatgaaa cggacataat ccacgccttg atagcccaac tgcgatgggt
     3841 caacattgaa accaaattcc ggacggtgcg tgattgcgtc c
//
LOCUS       DASZMM010000546         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3536183, whole genome shotgun sequence.
ACCESSION   DASZMM010000546 DASZMM010000000
VERSION     DASZMM010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536183"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VG754_13110"
     CDS             complement(<1..1301)
                     /locus_tag="VG754_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20201.1"
                     /translation="MKENHSGGPESALMGRIQGVLCLIASLLGRVGMALVALVLLAVL
                     FYQEENWHGKRVWARAQAEAAASGKVVDWAKFCPPPAPDDENFFKAPQMQQWFTGRGA
                     RDLSQRMGFQNFENSLNEPDTIVAMVTIVPPGKVVAPGEADIVLSYHGSSLTVSERPE
                     KVVTNKLSTEIIPLIVMDTVPLCDAIRNLAVQADLNYAIDPAIRFEVTLANGERIPQP
                     MVSLKITNQTALQVLDTILSTNNLALLPDAQSGSARIVAAGQNGSPGSHLEGPYITKV
                     IVEEESTNGTKSDEIPLIVMDDVPVTDAIKNLARQAGVKYALDPKINYGRPDAQGNIY
                     PQPTVSLRWTNLTAMHALKALLANYNLTWVPNPQTGIARVCIKDLYNDSLVDKELSAQ
                     ITKMLQAAFQPATNDEAGAHLTAALGFELSAKPLSATKPAR"
     gene            1499..>2302
                     /locus_tag="VG754_13115"
     CDS             1499..>2302
                     /locus_tag="VG754_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20202.1"
                     /translation="MSKFVWIALAFSFLLLFPAEYFARGEDQKTESRTPFVHNIPLRD
                     ADGQIISMPSLLVDGKLQDAKGNPFSMPDTCGKCHEYEIMGKGWHFNAAMGNVKPGRP
                     GEPWILTDPATHTQIPLSYRGWNGTFNPRDIGMNDFDFVATFARHLPGGGVGEPEKLD
                     AKDPNTRRMLVTGTLQVDCLICHDSSGRYTYEPRFNALKSQNFKWAPTIAEGLGTISG
                     FRTAKSIADSWRGTNSAPTNVPAIKYERDRFDADNNVLLQMTRKSSANAC"
BASE COUNT          514 a          666 c          593 g          529 t
ORIGIN      
        1 atgcgcgccg gctttgtcgc actgagtggt ttggcagaaa gttcaaagcc gagcgcggca
       61 gtcaaatgcg ctccggcctc atcattcgtt gcgggttgga atgcggcctg aagcatcttg
      121 gttatctgcg cgctcaattc cttgtcaacg agcgaatcat tgtaaaggtc tttgatgcac
      181 acgcgagcaa tgccggtttg cggatttgga acccaagtca agttgtagtt tgccaatagg
      241 gctttcaaag catgcatcgc ggtgaggttc gtccatctca atgacacggt cggttgcgga
      301 tagatgttcc cctgcgcatc cggccgacca taatttattt tcggatccaa cgcatattta
      361 acacctgctt ggcgggcgag atttttgatc gcgtcagtca ccggcacgtc gtccatgacg
      421 atcagcggta tctcatctga cttcgtgccg ttcgtggatt cctcctccac aatcaccttg
      481 gtgatatatg gtccctctaa atgcgaacct ggcgagccgt tctggcccgc agcaacaatt
      541 cgagcgcttc cggattgtgc gtcaggaaga agggcaagat tgtttgtgct tagaatcgta
      601 tccaatactt gcagcgccgt ctggttcgtg attttcaagg aaaccatcgg ctgcggaatc
      661 cgttctccat tggcaagggt cacctcaaat cgaatcgcgg gatcaatcgc gtaattcaaa
      721 tctgcttgaa cagccaagtt gcggattgcg tcacacaacg gcacggtgtc catcacgatg
      781 agaggaatga tttctgttga aagtttgttc gttaccacct tttccggcct ttcagaaacg
      841 gtcagtgacg agccgtgata actgaggaca atgtccgctt cgccaggcgc aaccacttta
      901 ccgggaggaa caattgttac catggccaca atcgtgtccg gctcattgag tgaattctca
      961 aaattctgaa agcccattcg ttgggacaag tcccgcgcgc cgcggccggt gaaccattgc
     1021 tgcatctgcg gcgctttgaa aaaattttcg tcatcaggcg ctggcggcgg gcagaatttc
     1081 gcccagtcca ccaccttgcc gctggcggcg gcttctgctt gcgcgcgcgc ccagacgcgc
     1141 ttaccgtgcc aattttcctc ctgataaaag agaacggcca ataacaccaa tgcaaccagc
     1201 gccatgccca cgcggcccag caacgatgca atcaggcaca atacgccctg gattcgcccc
     1261 atcaacgccg attccggccc gcctgaatga ttttccttca tgattctttg tgcttcagcg
     1321 ctccaaaatc agcacgttcc attccaaaca ccgcgcagca aagctagatt ccagctcttc
     1381 gcaacacgcg gagatgcaaa gtttaatccg caaatttctc tggcggcatg ggacgaaaat
     1441 gttttaaaag aggcgcgtgt cccgattgga attaacagtt tccgcagccc ggccaacggt
     1501 gagcaagttc gtatggatcg cgttggcgtt ttcattcttg ctgcttttcc ccgctgaata
     1561 tttcgcgcgc ggcgaggacc agaagaccga gagccgcact ccattcgtcc acaacattcc
     1621 cttgcgcgat gccgatggcc agatcatctc gatgccgtcg ttgctggtgg atggcaaatt
     1681 gcaggacgcc aaaggaaatc cgttctcgat gcccgacacc tgcggcaaat gccacgaata
     1741 cgaaatcatg ggcaagggct ggcatttcaa cgctgccatg ggcaacgtca aacccggtcg
     1801 tcccggcgaa ccgtggattt tgaccgaccc cgccacacac acgcaaatcc cgctttccta
     1861 tcgcggttgg aacggcacgt tcaatccgcg cgacatcggc atgaacgatt ttgattttgt
     1921 ggccactttc gcgcggcatc tgcccggtgg cggcgtgggc gaaccggaaa aactcgacgc
     1981 caaggacccg aacacccgcc ggatgctggt cactggcacg ttgcaagtgg attgcctcat
     2041 ctgccatgac agcagcggtc gttacactta tgaaccgcgg ttcaacgcgt tgaagtcgca
     2101 gaatttcaaa tgggcgccga ccattgccga aggattggga acaatttccg gttttcgcac
     2161 cgccaagtcc atcgccgatt cctggcgcgg caccaattcc gctccgacca atgttcccgc
     2221 catcaaatac gaacgcgacc gtttcgacgc cgacaacaat gtgctgctgc aaatgacccg
     2281 caagtcgtcg gccaatgcct gt
//
LOCUS       DASZMM010000547         3643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4637311, whole genome shotgun sequence.
ACCESSION   DASZMM010000547 DASZMM010000000
VERSION     DASZMM010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4637311"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(492..707)
                     /gene="infA"
                     /locus_tag="VG754_13120"
     CDS             complement(492..707)
                     /gene="infA"
                     /locus_tag="VG754_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWF20203.1"
                     /translation="MKEEHIEIEGCVQTVLPGTMFRVELDNKRLVLATISGKMRKRFV
                     RLTVGDRVKMEMSPYDLEKARITWRLK"
     gene            complement(834..1178)
                     /locus_tag="VG754_13125"
     CDS             complement(834..1178)
                     /locus_tag="VG754_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWF20204.1"
                     /translation="MSNNNKLFVGNLSFKITENDLQDTFAAHGTVIEANLMMDRATGR
                     PRGFGFVTMSTDEEAANAVSALNGKSIDGRALTVNIARPREERAPGGGGGRPQYGGGR
                     SGGGGGGGRNRY"
     gene            complement(1542..3161)
                     /locus_tag="VG754_13130"
     CDS             complement(1542..3161)
                     /locus_tag="VG754_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20205.1"
                     /translation="MQNPSSDPMTSSTTKHHHRFFSWRTLGLCLIGLAVLVTLIALFY
                     AEEDWRGKHAWEKYKRDSESHGVSFDLQKLIPPPVPDDQNFAMTPFLKPVLDLNPRPF
                     TSSPWRDTNAQQRAQNFGKEIIDHFNTPKPNGDNLAEGRMTDLPVIASGVELDQQKST
                     KNKHSKSKTPQEVATNFSRTEAATYLLDYMKRYDPVLDEIRAASQRPYSRFNVHYDDE
                     DPAGILLPHLGVLRRLANILQVRASAELALGNTASAFEDFQLLFYLGDSVKDEPIIIS
                     QLVRAAIFNTGIQIIWEGLNEHRWTDAQLQAFQTRLENVSLFKDGEKALQGERIAFGM
                     VFYDYARKHPEILGNVWGPGEPTGDIVLLQFAPDGWLYKEQIIQQQLVQRLIGAFDAE
                     KDLVHPEIAKSVVASTHDLGGAFSRLIHHESMVPLWMDGIGKYCQKPPFAQNNVNETV
                     IACALERYRMANGNYPDNLDALSPKFLDKIPFDVCTGQPMKYRQTAVGKFLLYSVGWN
                     EKDDGGTVVMDKQKTRIEDLSEGDWVWPKYP"
     gene            complement(3197..>3643)
                     /locus_tag="VG754_13135"
     CDS             complement(3197..>3643)
                     /locus_tag="VG754_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20206.1"
                     /translation="IIRGEPTMKTPREIIMERHRAAETKLDAIRKEELAALACASAIT
                     PKNSFNLSAAFERFWRESIWPWRRVWTGIAAVWLGILALNLAAGGTPQPGSSGMTATK
                     NPEVVTALLEQKKLLAQLLEPVMTSTPPSSKPPGPRSEQRQELFIA"
BASE COUNT          838 a          945 c          938 g          922 t
ORIGIN      
        1 caactttgaa ctttgaacct aaaaccttga actcgccgca aggcgggggg tatttttggc
       61 gagttaaagc gggtctaagt ggttctaagc gggttttcaa gtacttacga atgataatgg
      121 cttgggacgt gacacagctt ggctgccggg cgacacgagc gctggaattg agcaaacgaa
      181 gtttccttcc ggctgaagga tgaccggatt gccgaacacc aaaggtgtct cgtgtgctcc
      241 ttgtcttcac acgggacgga ttctgacacg gcggctgccg gttgtcgagg attttccaat
      301 gggtcagtaa aaggggcgca tccgctcaac gaatgtggta tcaggcatct cgcctgacgc
      361 agcaggcggc atcttgccgc ctatttggct tgcgaggaat ttttctgcgt ttcaaacgtc
      421 atggatcaac ccgccgggca ggatgcccgg ctttacggca aggcggggac gcctgccgcc
      481 actgcgcaaa tctatttcag ccgccaggtg atgcgggctt tttccagatc gtagggagac
      541 atctccattt tcacgcggtc gccgacggtc aatcgcacaa agcgcttccg cattttgccg
      601 gagatggtgg cgaggacgag gcgtttgttg tcgagttcca cccggaacat cgtgcccggc
      661 aacacggttt ggacgcatcc ttcaatctcg atatgttctt ctttcatggc attattccgc
      721 aaaactagcc ggtctgatgt gaaagacatg cttcagcaga ccaggaatgc agaagtgcct
      781 caaaaaaaat cgaggcccgg cgaacccggg cctcgaaact gcaaaggacg cgattaatag
      841 cgattgcgtc cgccaccacc gccgccgcca ctacgaccgc cgccatattg ggggcgtccg
      901 ccgccgccgc cgggagcgcg ctcttcgcgc ggacgggcga tgttcacggt gagggcgcgt
      961 ccgtcaatgg acttgccgtt caatgcggaa acggcgttcg ccgcttcctc atcagtgctc
     1021 atggtgacga aaccaaaccc gcggggacgt ccggttgccc ggtccatcat caggttggct
     1081 tcaatgacgg tgccgtgtgc ggcaaacgtg tcttgaaggt cgttctcggt gattttgaag
     1141 gaaaggtttc ctacaaacaa tttgttatta ttactcatgt gatgtctctg taccgactga
     1201 attgcttttc ccagactgtc cccggtattc gggtttaaaa tcatcactga actgagttca
     1261 cctgaggatt tgccatatct tgaaagtgac aagctgatcg aacagacaat caactatgcc
     1321 gttatttgcg agaatagcaa atggaatttg cgataatatt tgcggaaaaa tatcggcaca
     1381 tgacttcaaa tccatcaggt gtgcagacat ctcccgtcac taacttgata tagggcgagg
     1441 tctcaaaaaa tgggtaaaac ctcaactata tcgttatcga aaaagcctgt ttaacccact
     1501 tttcacaaaa tcttcagttc acagacctgt aaaaagcatc atcacggata tttcggccaa
     1561 acccaatctc cctcgctcaa atcctcgatc cgcgtttttt gcttgtccat cacgaccgtt
     1621 ccgccatcgt ccttttcgtt ccagccgacg gaatagagca gaaattttcc aacagcagtc
     1681 tggcgatatt tcatcggctg gccggtgcaa acgtcgaatg gaattttgtc caggaatttt
     1741 ggcgaaaggg catccaagtt gtcgggataa tttccgttcg ccatgcgata gcgttccaac
     1801 gcgcaggcga tgacggtttc gttcacgttg ttttgggcga atggcggttt ttgacagtac
     1861 ttgccaatac catccatcca taacggaacc atggattcat ggtgtatcaa ccgcgaaaac
     1921 gcgccgccga ggtcatgcgt acttgcaacg actgattttg caatttcagg atggaccaga
     1981 tctttttccg catcgaatgc ccctattaat cgttgaacaa gctgctgttg aatgatttgt
     2041 tccttgtata accacccatc aggagcaaac tggagtaaca caatgtctcc cgtcggttcg
     2101 cccggccccc aaacattacc caggatttcc gggtgcttgc gcgcgtaatc ataaaaaacc
     2161 atgccaaacg cgatgcgctc tccttgcaaa gccttttcac catccttgaa gagcgaaaca
     2221 ttttccaacc gcgtttgaaa agcctgcagt tgtgcatccg tccagcgatg ttcattcagc
     2281 ccttcccaaa tgatttggat gccggtgtta aagatcgcag cgcgcaccaa ctgcgagatg
     2341 atgatcggct cgtctttgac cgagtcgccc aggtaaaaga gcaactgaaa atcttcaaac
     2401 gccgaagctg tattgccaag cgcaagttcc gctgaagctc tgacttgtag aatgttggcc
     2461 agccttctca acacacccaa atgaggcagc aaaatcccgg cgggatcttc gtcgtcgtaa
     2521 tggacattga atctggaata tggtcgttgg cttgccgcgc gaatttcatc cagaaccgga
     2581 tcgtagcgct tcatgtaatc cagaagatat gtcgcggctt ccgtccggga aaagtttgtc
     2641 gccacttctt gcggagtttt ggatttggag tgtttatttt tggttgattt ttgttgatcc
     2701 aattcgacgc cggaagcgat gaccggcaaa tcggtcattc gtccttcggc gaggttgtcc
     2761 ccattcggtt tgggcgtgtt aaaatgatca atgatttcct tgccgaaatt ttgagcgcgt
     2821 tgttgggcgt tcgtatcgcg ccagggcgaa ctggtgaatg gccgcggatt caaatcgagc
     2881 accggcttca aaaacggcgt catggcaaaa ttttggtcat cgggcaccgg tggcgggatg
     2941 agtttttgca gatcaaaact gacgccgtgc gactccgagt cccgcttgta tttttcccac
     3001 gcatgttttc ccctccaatc ctcctcggcg tagaagagcg caatcaaagt caccaacacg
     3061 gcaagtccga tcaggcagag tcccaaagtc cgccaactga aaaatctgtg atgatgcttg
     3121 gtggttgatg atgtcatcgg gtccgaggat ggattttgca taatattttt ttctccctaa
     3181 acaaaatgcc gatgactcag gcaatgaata attcctggcg ctgttcgctt cgcggtccgg
     3241 gcggtttcga ggaaggaggt gtcgaagtca tgaccggctc caataattgc gccagcagtt
     3301 ttttctgttc gagcaatgcg gtcacaactt cagggttctt agttgcagtc atgccgctgc
     3361 tgcccggctg aggtgttcca ccagccgcca gattcaacgc cagaatgccg agccacactg
     3421 cggcaattcc ggtccacacg cgacgccatg gccagattga ctcacgccaa aatctttcga
     3481 acgccgcact caagttaaat gaatttttgg gggttatcgc ggatgcgcag gccaatgctg
     3541 cgagttcttc ctttcggatg gcgtcgagtt tcgtttcagc ggcgcgatgc cgttccatga
     3601 tgatttcacg cggggttttc atggttggtt ctcctcgaat aat
//
LOCUS       DASZMM010000548         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3537212, whole genome shotgun sequence.
ACCESSION   DASZMM010000548 DASZMM010000000
VERSION     DASZMM010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3537212"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VG754_13140"
     CDS             complement(<1..1085)
                     /locus_tag="VG754_13140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWF20207.1"
                     /translation="MSYCRKLIWALALVILASCTTPPPRQTPPDVDLSPDQPVVAKPE
                     VVVIKPPKAIAPAPAPTPAPLVIPPQDHIGPNEVWVPLERWCQACGYGAPRRIGNDPG
                     PAYAFTLTNGTMTVRIGVELATWRGMDYHLGFAPELVNGHPYIHALDIRKNFLPLLEN
                     SIPLETNRVIVIDPGHGGTDTGTSNVANGHFEKEFTLDLARRLQALLAADGWTVLLTR
                     TSDVYVSLPNRVAFAEQHKAGLFISLHFNSSFPDRQEAGLETYCLTPHGMRSTLTRGF
                     ADNPSLTFPNNNFDAENLEFAASVHREILKINGHLDRGLRRARFLGVLQGQNRPAFLV
                     EGGYISNPTEARKIADPAYRQKLAEAIA"
     gene            1190..1753
                     /locus_tag="VG754_13145"
     CDS             1190..1753
                     /locus_tag="VG754_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HWF20208.1"
                     /translation="MSLRIVVAITGASGALYAQRLLDNLDPRQHEVHVVMSNYAQTVI
                     TEELPGGLRLAPGVKTHGLKSMNVPFVSGSNPFDAMVVIPCTMGTLGRIAHGYSEDVL
                     LRAADVMLKERKKLILVPREAPLSLVHVKNFELLLLAGATLLPANPFFYTHPKTIEQV
                     VDTVVARVLDHLGVPQNLVARWAEEKD"
     gene            1817..2707
                     /locus_tag="VG754_13150"
     CDS             1817..2707
                     /locus_tag="VG754_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HWF20209.1"
                     /translation="MSLLETWGSFVKFSHTVFALPFALAAMVVAARDHRGWPGWKTFG
                     LILLAMVSARTCAMAFNRIVDRKFDALNPRTKNRHLPAGQISLMNAVLLCVISGVGLV
                     AASYFLNAVCFYLSPFALAVICFYSLTKRFTDYTHIFLGLALALAPVGAWLAVKGANI
                     SPLEIIQMLTLACAVVLWLVGFDIIYALQDYEFDKMHGLRSLVVAWGPKNALVAAFLA
                     HMLMCGLLFAFGLLCRFRVAYVVGWLIIVGCLALEHWIARRRSLNWINLAFFRLNAVV
                     SAVFFFVTTAEVVFPFNFGR"
     gene            2776..3021
                     /locus_tag="VG754_13155"
     CDS             2776..3021
                     /locus_tag="VG754_13155"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HWF20210.1"
                     /translation="MVIQIQDEVLQGLGLTESQALLDLAVGLFTEQRVTLARAAQIAR
                     VTQMDFQKELGKRGIPIHYDVEDLQSDLRNLAALREK"
     gene            3078..3479
                     /locus_tag="VG754_13160"
     CDS             3078..3479
                     /locus_tag="VG754_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HWF20211.1"
                     /translation="MLRELHKRILIPPAVGRELASSHQLIPAFVETCEVSNRQMVAQL
                     EAELDLGEAEAIVLAKEKHADLLLIDEKRGRQIAVREGIRISELMALLVEAKHQKFLV
                     SVRDTVVQLETKAGFRVSEAVKLAVFHAANE"
     gene            3517..>3947
                     /locus_tag="VG754_13165"
     CDS             3517..>3947
                     /locus_tag="VG754_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HWF20212.1"
                     /translation="MSFLIQRSGLRDIYEKVVAGERISDEDALRLFESKDLNAIGAIA
                     DFARQKKVGNRASYILNRYLNYSNYCILSCQFCSFARKKRDADGFQLSVGEMAQKARE
                     ALAIGITELHIVGGLHPSLPFSYYTDLLRTLRGVDARLQLK"
BASE COUNT          812 a         1084 c         1111 g          940 t
ORIGIN      
        1 gcgatggcct cggccaattt ttggcggtaa gcggggtccg cgatcttccg cgcttcggtt
       61 gggttggata tatatccgcc ttccaccaaa aatgcgggcc ggttttgccc ttgcaacaca
      121 cccagaaaac gcgcccgccg caagccgcga tcaagatgcc cgtttatctt caagatttcc
      181 cggtgcaccg aggcggcaaa ttcaaggttc tccgcgtcaa aattgttgtt cggaaatgtc
      241 agcgaaggat tgtccgcaaa accgcgcgtc aacgtcgagc gcatcccatg cggagtgagg
      301 caatacgtct ccaggcccgc ttcctgccgg tcgggaaaac tggaattgaa atgcaggctg
      361 ataaacaatc cggccttgtg ttgctccgcg aatgccaccc ggttcggcag cgagacatac
      421 acatcgctcg tgcgcgtcag caataccgtc cagccgtcgg ccgcgagcag cgcttgcaaa
      481 cgccgcgcca ggtcgagcgt gaattccttt tcgaaatggc cgttcgccac gttgcttgtg
      541 ccggtgtccg tgccgccatg tccggggtcg atcacaatga cacggttggt ttccagcgga
      601 atggaatttt ccaacagcgg cagaaaattt ttgcggatgt cgagcgcatg gatgtatgga
      661 tgtccgttca ccaattccgg cgcaaagcca aggtgataat ccattccgcg ccaggtggcc
      721 aattcgacgc cgatccgcac ggtcatggtc ccgttcgtga gtgtgaaggc ataagcgggg
      781 ccggggtcgt ttccgatacg ccgcggcgcg ccatagccgc aagcctggca ccatcgttcc
      841 aaaggcaccc aaacctcatt tggaccgata tgatcttgcg gtggaatcac cagcggggcg
      901 ggtgtgggcg ccggagcggg agcgatggct tttggcggct tgattacgac aacttccggc
      961 ttggccacca caggttggtc gggcgacaaa tcaacatccg gcggcgtctg gcgcggcggc
     1021 ggcgttgtgc aactcgcgag aatcaccagc gccagcgccc aaatcaattt acggcaataa
     1081 gacacccctc tattgtgcga aagaatgccg gggaaacaag aagttttgac gaaaagaaag
     1141 cgacccaacc cctgcttgcc ggaaactgtt ttaacagcca tcatgggcct tgagcttgag
     1201 aattgtcgtc gccataaccg gagccagtgg agcgctttat gcgcaacgcc tgctcgacaa
     1261 cctcgacccg cgccagcatg aggtgcatgt ggtcatgagc aactacgccc aaacggtcat
     1321 caccgaggaa cttcccggcg ggttgcgcct cgcgcctggc gtgaaaaccc acgggctcaa
     1381 gagcatgaac gtcccgttcg tcagcggttc aaatccgttc gatgccatgg tcgtcatccc
     1441 ctgcacgatg ggcacgctgg gacgcatcgc gcatggttac agcgaggacg ttttgttgcg
     1501 cgccgcggac gtgatgttga aggagcgcaa aaaattgatt ctcgtgccgc gcgaagcgcc
     1561 gttgagcctt gtgcatgtga aaaattttga actgctgctg cttgccggcg cgacgttgct
     1621 tcctgccaac ccattttttt atacgcatcc caaaaccatc gagcaggtgg tggataccgt
     1681 cgtggcaaga gtgttggacc atctcggcgt gccgcaaaac ctcgtggcgc gctgggccga
     1741 agagaaagat tgaggataaa cgtgcaattc taacatgtcc tctccatcct ctatcctcca
     1801 tcctccatcc tcgcgtttgt ccctcctcga aacgtggggt tcgttcgtga agttctcgca
     1861 taccgtcttc gcgttgccgt tcgcgctggc ggcgatggtc gtggccgccc gggatcatcg
     1921 cggctggccc ggctggaaaa cctttgggct tattttgctg gccatggttt ccgcgcgcac
     1981 ttgcgcgatg gcatttaacc gcatcgtgga ccgcaaattt gatgcgctca atccgcgcac
     2041 aaaaaatcgc caccttcctg ccgggcaaat ttcgctgatg aacgcggttt tgctttgcgt
     2101 gatttccggc gtcggattgg ttgcagccag ttattttctc aacgctgttt gcttttatct
     2161 gtcgcctttc gcgctcgcgg tgatctgctt ttattcgctg acgaaacggt ttacggatta
     2221 cacccacata tttcttggcc tcgcgctcgc gctcgctcca gtcggcgcgt ggctggccgt
     2281 gaagggcgca aatatttctc cattggaaat catccagatg ctcacgctcg cctgcgcggt
     2341 ggttttgtgg ctggtggggt tcgacatcat ttacgcgttg caagattacg agttcgacaa
     2401 gatgcacgga ctgcgttcgc tcgttgtcgc atggggaccg aagaacgcgc tggttgccgc
     2461 gttcctggcg cacatgctca tgtgcgggtt gctgttcgca ttcggattgc tctgtcgttt
     2521 tcgcgtcgcg tatgtcgtcg gatggcttat catcgtcggt tgccttgccc ttgaacattg
     2581 gattgcccgc cgtcgcagcc tgaactggat caaccttgcg ttcttccgcc tcaatgcggt
     2641 cgtcagcgcg gttttctttt ttgtgacaac cgctgaggtg gtttttccgt ttaacttcgg
     2701 gcgatgaacc aattgtttgc caatcttgca tcgtgttcgg cttgccaaca tggatgagaa
     2761 aggtatgata ttggcatggt aattcaaatt caggatgagg ttttacaggg cttggggctg
     2821 actgaatcgc aagccttgct cgaccttgcc gtgggtttgt tcaccgaaca acgcgtcacg
     2881 ttggcgcgcg cggcgcaaat cgctcgcgtg acacaaatgg attttcagaa ggaactcggc
     2941 aagcgcggca ttcccattca ttacgatgtt gaggatttgc aatcagactt gcgaaacttg
     3001 gccgcattgc gtgaaaaatg attgtcatca gcgacacgtc ggtgatcacc tcgctgcttc
     3061 aaatcggccg gctggatttg ctgcgcgaac ttcacaaacg aatcttgatt ccgcctgcag
     3121 tcgggcggga actggcgagt tcacatcaac tgattcccgc ttttgtcgag acatgcgagg
     3181 tgtcaaatcg ccagatggtt gcgcaacttg aggccgaatt ggacttggga gaagccgagg
     3241 ccattgtcct ggcgaaagaa aagcacgccg atttactttt gattgatgaa aaacgtggcc
     3301 gccaaattgc cgtccgcgaa gggattcgca tttcggagct gatggcattg ttggtggagg
     3361 ccaaacacca gaaattcctt gtatcagtgc gggatacagt tgttcagctt gaaacgaaag
     3421 ctggcttcag agtttcagag gctgtgaaac tggccgtctt tcatgccgca aatgaatgag
     3481 aacccatcta cattttgatt ttatcgagtg acacccatga gctttttaat ccaacgcagc
     3541 ggcttgcgcg acatctacga gaaggttgtc gcaggcgaac gcatttcgga cgaagacgca
     3601 ttgcggctgt tcgaatcgaa ggacttgaat gccattggcg cgatcgctga ttttgcccgc
     3661 cagaaaaaag tcggcaaccg cgccagttac atcctcaacc gctatctcaa ttattccaat
     3721 tactgcatcc tgagctgcca attctgctcg ttcgcccgca aaaaacgcga tgcggacggc
     3781 tttcagcttt cggtcgggga gatggcgcaa aaagcgcgcg aagcgctcgc catcggcatc
     3841 accgaattgc acatcgtggg cggattgcat ccttcgctgc cgttcagcta ttacaccgac
     3901 ttgttgcgca cattgcgcgg cgtggatgcg cggctgcaat tgaaatg
//
LOCUS       DASZMM010000549         8968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5471938, whole genome shotgun sequence.
ACCESSION   DASZMM010000549 DASZMM010000000
VERSION     DASZMM010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5471938"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..2800)
                     /locus_tag="VG754_13170"
     CDS             complement(2..2800)
                     /locus_tag="VG754_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20213.1"
                     /translation="MKLEFSNALLKWYWEFGSRRTDKYSLPLSGWVHDLYEARDGFAA
                     AVTASLDTKPAFAIWGPSQTGKSMLVSAYFDQMAVRKRVAGEDGKDSALYWPGGLPCY
                     FIMPDEFKSDPPPWINLLNPFRLGKDASACLSRFSIGSTTPVPGRHYVMSPQYPVELK
                     LLNQTELLHVLGRGYDTECLGPLNNGTATIWTVDEFRDRVQAVKKRFPSRPGQLNQAA
                     VELLIDVCGVFDSMVLAELMRFQPLVAREGVENWKNILAGLLDDPQFTSNPELVEALA
                     ADVLWDSAAPLTDAHRKLKRALAAFEEMWGKRTIHCDLQVAALLLDMDSVLVAFDGPP
                     PDATAMNRDRQIAEQIRQLRFKIDGDHVYLGLSPALPNLLNLSPEDYGHLQSLVLELV
                     IPVNPDFLEESNFKKFLDRADLLDFPGVGNDSNAKFTRIDLGFGNAPTVNDVPVDIPL
                     ENRFPKYNPRHLFTRILKRGKTATIVSLYARKLKIDGFSLFLALDKNPPAKPSELNEG
                     INTWWKCAVPSYYKGGRNTKSPLPLNLVLLWWANLINEAGPAAANFMARVKWIYDPLG
                     DISNPAVSNFFTLNYYRIAHRGGVLDQSKLKRDSFFVRGILAEPEFKRVFGVPESTGY
                     RSFMRMIEDAETGGAEFYFNELCLQLEKPVLRRDQILKGVAEVSKEKIISLLAAKDLF
                     PEPEERDVRREILEAMEAEILALIDASDDRTITQINHLLRETLNIDFRALKPVPMFPH
                     EVNTDFLRSQFASWITSQCNRVEAWRKNGRRDKPDWSLLGLDSREKLRSWLEALVASL
                     EGHLLPMAVWLKDLVEYNLDRPGTDLRRILAIRMGNVLIYGKSGPPSYDSGIGDSASF
                     DDISVDPKGRTLKGRGCPAYKVFLQPFLEAQLKKLLNEGIGLIQRQAQPGDEQLRKLC
                     MDNDCLPACAQSPDESKK"
     gene            complement(3020..5839)
                     /locus_tag="VG754_13175"
     CDS             complement(3020..5839)
                     /locus_tag="VG754_13175"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWF20214.1"
                     /translation="MNARFIYPTNLRLFTASAILFYLCLAPAFAQTTLTNGLVAYWSF
                     DTTDGTSTPDLALNNSLFLFNSPANGASGNTVRNTGNCFTFNGSSQYLDLSHSTNFAA
                     TGLPVYTTHGYTMAFWVNGPVQAANHIIFSDNNFNNGGLLFEFNTSTGAKIRVNVRDD
                     NNVTRLSNVTSTGNAFDNTWHHIAWTDTNGAAKLYIDGNLDSSFSYTASAAPPTGNVL
                     SVGALVRSSGVGNYFNGSVDEVMAWNRVLSQTEIQNAMSNGIPGPIVATPPTVVNQPA
                     NHTNSLGDMVLLSANVFGNQPMSYQWLSNGMVVPNQTNSTLELYTLTSPGTNFYSLFA
                     TNSAGSNTTSVASVVVSPDPAANLNLGMVSYWPMDTISNSTSTPDLVSQNDFQLANMS
                     GTNLVSGEFGNALAFDGATQYGIEATGTPIYDVSTTYTVALWVKGPSGRANEQVFANG
                     HTNGNYFFIGPDNAGATGKADVRINPGLGDTLSTATAFDGTWHHVVWVDQNGSGLLYI
                     DGNLDPTPYNYTHGTLGSVSLTNTTVGALVANSLRDFYAGSVDDVGTWNRRLTYTEIQ
                     TIYHSGIPAPPVIVKPSISPIITVPASLSNDVYQGDTVSFSASVSGTSPFSYQWRTNG
                     VPITGNPSALTNKLVLTHVQPGNSAAYALVVTNQGGSATSSVVQLSVIPYVPATNGTV
                     LQLEFNPVLNPVVQTGFSSMTLNSNPSTFGGPMVTLSTIGATGLGDRLRTVPTNNPPA
                     LTTADIYSQIIFSTANSFGTGIDILVQRLAPNTSYGVTLWSWDSQNSGSSVWNEIASG
                     TPVSFVYLNNNSSYFFSGSPMPAADYDDTLGGLLTSSASGQLEFQGTQNGPSLSVFIN
                     ALRLVANPTVQITHTTIAADSNLQLTISMQYPNQSFHFQESTTLSPGSWQDATDAQVI
                     STHGPVSVVEFPISSTQLFYRVVSP"
     gene            6095..7285
                     /locus_tag="VG754_13180"
     CDS             6095..7285
                     /locus_tag="VG754_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20215.1"
                     /translation="MIALFFAIEAGRVHAQNQNYSSRIDYDVLKREQRGLVNVLTDGE
                     TDLSSGETRLRNNSLRLSAEEVARAASKVAHLRAMAQLHPERCARTTGAFLLPAGAAL
                     QSLTNNFFGNIKPRIVRLEDNLDAYVTQLERLDPKLAAELRSLIDAYNRAAFDIVNNA
                     ANGSGSIEYNPSSIDAAVAPNLRGNKIFENINRSGGGGTSGGGAGDPNDPNNPNSPNY
                     RGNGANDPNNPNYRGGNGANGGNGVINPGSFHGTIRDTGGQILNADDFLDNKTVVKRT
                     KSDNGILTREEKTSGDIVPDGNGGAVFRKRDSKAIDWQFDIEANENGFALVDRSNGPA
                     DEDKGFSVQSWTVKDPSGQTQDFPGTEQLKYAMSAPGKYTITAKVLTKLKTNFTIYIS
                     PVNN"
     gene            7385..>8968
                     /locus_tag="VG754_13185"
     CDS             7385..>8968
                     /locus_tag="VG754_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20216.1"
                     /translation="MIPTRHAELLNLPPETSPREAQGDCLPLQQELLAINPALPPPKK
                     PLSGPEEVLNDVSNGAPPETAPTPQSGAEFCGTVPVEFLSVLDLDENANAEQALAKAQ
                     ARLKETVDAQSSLKPRHPAHPKLKRDRARFEQMTAELREIVFSGKVDALCDRTEEALE
                     CRPPNRAKARDCVRRARTEGTHLQPESACLRRLQKLEVKLEGGDADSATAPISNETAS
                     APASVHAAPPFVAPVENPFLRLESLVDKGMACLAQDYPDVSGAIESSAEAQAEADGKE
                     VPSNLRSRMQLFKRTLEQVRREAIHARVDECLRQAAAAMAQDPPATDETREKLHLAES
                     LLKEAPDSYFRSEVARLERVIEEHEHWLSTRRSNEMELLAETRPAPPPIEAHPLPAKD
                     DVYPTMPWPRAPKKISVKVNPKARAQILPLPPPGMAPMAPEMTPQPPVQPVVPLKPTK
                     RSKALEIGVALLLIGLISAACASFRIAGMLSTLMGHSGNIQSNQPVQPSEFFAEKPAS
                     QTSAPLFSRRPEIGELSVSV"
BASE COUNT         2098 a         2387 c         2570 g         1913 t
ORIGIN      
        1 ctcatttttt ggattcgtcg ggagattgcg cgcaagcggg caggcaatcg ttgtccatgc
       61 agagtttgcg gagttgttcg tcgccgggtt gggcctggcg ctggatcaaa ccgatgcctt
      121 cgtttaaaag ttttttcaac tgcgcttcaa ggaaaggttg caggaaaact ttgtaagctg
      181 gacaaccgcg acctttcaac gtgcgacctt tggggtccac cgaaatatca tcaaagctgg
      241 cgctgtcgcc gatgccggaa tcgtaggatg gcgggccgct tttcccataa atcagcacgt
      301 tgcccatccg aatggccagg atgcggcgca aatcggtccc gggacggtca agattgtatt
      361 ccaccaaatc tttcagccag acggccatcg gcaacaagtg gccttccaac gaagccacga
      421 gcgcttcaag ccagcttcgc aatttctccc gcgaatccaa tccgagcagc gaccagtcgg
      481 gtttgtcgcg gcggccgttt ttgcgccatg cctcgacgcg gttgcactgg ctggtgatcc
      541 aactggcgaa ttgcgaacgc aggaaatcgg tgttgacctc gtgcgggaac atcggcaccg
      601 gcttgagcgc gcggaaatca atgttcaatg tttcgcgcaa gagatgattg atttgcgtga
      661 tggtgcggtc gtcactcgcg tcaatcaacg cgaggatttc cgcttccatc gcttcaagta
      721 tttcgcggcg gacatcgcgc tcctcgggtt ctggaaaaag gtccttcgcg gcgagaagtg
      781 aaatgatttt ttccttcgaa acttcggcca cgcccttgag aatctggtcc cgccgcagga
      841 ccggcttttc aagctgcaaa caaagttcgt tgaaataaaa ctccgcgccg ccggtctcgg
      901 cgtcctcgat catccgcatg aacgagcggt atccagtcga ttctggaacg ccgaagacgc
      961 gcttgaactc cggctcggcg aggatgccgc gcacaaaaaa actgtcgcgc ttgagcttgg
     1021 actggtcgag cacgccgccg cgatgggcga tgcgataata attgagcgtg aaaaaattcg
     1081 acacggcggg attggagatg tcgcccagcg gatcgtaaat ccatttgacg cgcgccatga
     1141 aattggccgc ggcggggccg gcttcattga tgagattggc ccaccaaagc aacaccaggt
     1201 tgagcggcag cggactcttg gtgttgcgac cgcccttgta ataactgggc accgcgcatt
     1261 tccaccaggt gttgatgccc tcgttcaatt cgctcggctt ggccggcgga tttttgtcga
     1321 gcgcaagaaa gaggctgaac ccgtctatct tcagcttgcg cgcgtaaagg gaaacgatgg
     1381 tcgcggtctt gccccgcttg aggatgcgtg tgaatagatg gcgcggattg tatttcggaa
     1441 aacggttttc gagcgggatg tccaccggca catcgttaac ggtcggcgcg ttgccgaagc
     1501 cgaggtcaat ccgggtgaac ttggcgttcg aatcattgcc cacgccggga aagtcgagca
     1561 agtctgcccg atcgaggaac tttttgaaat tcgattcctc cagaaaatcc ggattcactg
     1621 ggatgaccaa ttccagtacc aggctttgca gatgcccgta atcttccggc gagagattga
     1681 gcagatttgg caacgcgggc gagaggccga gataaacatg gtcgccatca attttaaaac
     1741 gcaattgacg gatttgttcc gcgatttgac ggtcgcggtt catcgcggtc gcatccggcg
     1801 ggggaccgtc aaaggcgaca agcaccgaat ccatgtcgag caacaacgcc gcgacctgca
     1861 agtcgcaatg gatcgtgcgt ttgccccaca tctcttcaaa cgccgcgagc gcgcgcttaa
     1921 gtttacggtg ggcatcggtc agcggcgcgg cagaatccca caacacatca gcggcgaggg
     1981 cttcgacgag ttcgggattc gaggtgaatt gcggatcatc gagcagaccg gccagaatgt
     2041 ttttccagtt ttcaacgcct tcgcgcgcga cgagcggttg gaagcgcatc aactctgcca
     2101 gaaccatcga atcgaacacg ccgcaaacat cgatcaataa ctcaaccgcc gcttgattca
     2161 gttgtcccgg cctcgaagga aagcgttttt tgacggcttg gacgcgatcg cgaaattcat
     2221 ccactgtcca gatggtcgcc gtcccgttgt tcagcggtcc gaggcattcg gtgtcgtaac
     2281 cccggccaag gacatgcaat aattcggtct ggttgagtag tttgagctcg accggatatt
     2341 gcggcgacat cacataatgc cgtcccggca ccggcgtggt gctgccgatt gaaaaacggc
     2401 tgaggcaagc agaagcatcc ttgccgagcc gaaacgggtt caaaagattg atccatggcg
     2461 gcggatcgct cttgaattcg tccggcatga tgaaatagca cggcaaaccg cccggccaat
     2521 agagcgctga atctttccca tcctcgcctg cgacacgttt cctcaccgcc atttggtcga
     2581 aataagccga aaccagcatg gatttgccgg tctgggaagg gccccagatg gcgaaagcag
     2641 gttttgtgtc gagggaggcg gtgacggcgg ctgcgaatcc gtcacgcgct tcgtataagt
     2701 catgcaccca accgcttaag gggaggctgt atttgtcggt gcgccgggag ccgaactccc
     2761 aataccattt gagcagcgcg ttgctaaatt caagtttcat tgccccgctg gacttgttac
     2821 ctcacgtaaa gatgtggcgc aagtaaattc ggccttttgg ggacgcatag ttgcttgttt
     2881 tggacagaga taggtgggtt ggaactcaaa atttacacag gccgcctctt agaaagatta
     2941 cgatgcggga ggcgtcaagc tgcataatgc aacgatgtat ctcgtaataa tattaagcac
     3001 taacaatccc gccgcgcact cacggactga ccacgcgata aaacaactgg gtcgaactta
     3061 tcgggaattc gaccactgaa accggcccgt gagttgaaat gacctgcgca tcggtggcgt
     3121 cctgccaact gccggggctc agggtagtac tctcctggaa gtggaaggat tgattgggat
     3181 attgcatgga aatggtcaat tgcagattgc tgtccgcagc gatggttgtg tgagtaattt
     3241 gaacggttgg attggccacc aaacgcaagg cattgatgaa aacactgagc gatgggccgt
     3301 tctgcgtgcc ctgaaactcc agttggccgc ttgctgacga ggtcagcaac ccgcccagag
     3361 tatcgtcata atcggcagcc ggcatgggcg atccgctgaa gaaatacgag gaattattgt
     3421 tgagataaac gaacgaaacg ggtgtgccgg acgcaatctc gttccacacc gaagagccgg
     3481 agttttgcga gtcccacgac cagagagtca caccgtaaga tgtattcggc gcaagccttt
     3541 ggacgagaat atctatgccg gttccaaagc tgtttgcggt ggagaaaatg atttgcgaat
     3601 agatgtccgc cgtggtgaga gcgggaggat tgttcgtcgg aacggtgcga agacgatcac
     3661 ccaaaccggt cgctccaatg gtcgaaagtg tcaccatcgg tcctccaaac gtggacggat
     3721 tcgaattgag cgtcatggaa gaaaaccccg tttgcacgac tggattcaag acagggttga
     3781 actccaattg caataccgtc ccattggtgg cgggaacata cggaatgacg ctcaattgaa
     3841 cgacgctgct ggtcgccgag ccgccttgat tggtcaccac cagcgcgtaa gcggcggaat
     3901 tgccgggctg gacatgggtc aggacgagct tgtttgtcag cgcgctcgga ttgccggtga
     3961 tgggcacgcc attggtccgc cattggtagc tgaacgggct tgttcccgag acgcttgcgc
     4021 tgaaactcac ggtatcaccc tgatagacat cattgctcaa gctggccggc acggtgatga
     4081 tgggagaaat gctcggtttg actatgaccg gcggcgctgg aatgccgctg tgataaatcg
     4141 tttggatttc cgtgtaagtc agccgacggt tccaagttcc aacgtcatcc accgagcccg
     4201 cgtaaaaatc ccgcaatgaa tttgccacca acgcgccgac ggtggtgttg gtcaaactca
     4261 ccgagccaag cgttccgtgc gtgtaattgt aaggcgttgg gtcgagattt ccgtcaatgt
     4321 acagcaaccc gctgccattt tggtccaccc aaacgacgtg atgccatgtc ccgtcgaatg
     4381 cggtcgcggt ggacaaagta tctcccaaac ccggattgat tcgcacatcc gctttgccgg
     4441 ttgcgcccgc attgtccggg ccgatgaaaa aataattccc atttgtatgg ccattggcaa
     4501 aaacctgttc attcgcacga ccggatggcc ctttcaccca aagggcgacc gtataagtcg
     4561 tgctcacgtc ataaatcggc gtgccagtcg cctcgatccc gtattgtgtc gcgccgtcga
     4621 aagcgagcgc attgccgaat tccccgctga caaggttcgt cccgctcatg ttggcaagtt
     4681 gaaaatcatt ctggctgaca aggtcgggcg tggaggtgga gttggaaatc gtgtccatgg
     4741 gccagtacga aaccatgcca agattgaggt tcgctgccgg atcaggcgat acgaccaccg
     4801 aggcgacgct tgtcgtgttg ctgcccgccg agttagtggc gaacagggag tagaaattcg
     4861 tcccgggaga agtcaacgtg tacagctcga gcgtgctgtt ggtttggttg ggcaccacca
     4921 tgccgttgga aagccattga taggacatcg gctgattgcc aaaaacgttg gcggaaagca
     4981 ataccatgtc gccgagggaa ttggtgtggt tggcgggctg gttgaccacg gtgggaggcg
     5041 ttgcaacgat aggccccgga atgccgttgc tcatggcgtt ttggatttct gtctgcgaca
     5101 gcacgcggtt ccatgccatc acttcatcca ctgagccgtt gaaataattg ccaacaccgc
     5161 tggagcgcac cagcgcgccg acactgagca catttcccgt cggcggcgcg gcggaagccg
     5221 tgtaactgaa cgaactatcc agattgccgt cgatgtagag tttggccgcg ccgtttgtgt
     5281 cagtccatgc tatatgatgc catgtattgt cgaatgcgtt accggtggaa gtgacgttgg
     5341 aaagacgggt cacattgtta tcgtcccgga cgttgacgcg tatcttggcg cccgtggatg
     5401 tgttgaattc gaataatagg ccgccattat taaaattatt gtcggaaaaa atgatgtgat
     5461 tggctgcttg tacagggcca tttacccaaa atgccatggt ataaccatgc gtggtgtaaa
     5521 ccggcaatcc tgttgccgca aaattggtgg aatgagacaa gtccagatat tgggaagagc
     5581 cgttgaaagt gaaacaattg ccggtattac gcactgtgtt tccactcgcg ccattcgcgg
     5641 gagaattgaa caggaacagc gaattgttca aagccaggtc cggcgtgctt gtgccatccg
     5701 tggtgtcgaa tgaccaatat gcgaccagtc cgtttgtcaa agtggtttgg gcaaaggccg
     5761 gggcaaggca gagataaaac aagattgccg atgcggtgaa aagacgaagg ttcgtggggt
     5821 atatgaaacg cgcgttcatg gcatgatcgg gttgaaagat ggggtccgag cagattgtgc
     5881 ctaatgcgga aaaatggtca agctaattgc cgattgcttc ttaaaaatta attttgagaa
     5941 aaacagaatc ctcaattatt agctggattt ctttcgcatt cagttggtta aataacagtt
     6001 gcacgtaaac ggaaagcaga taagttagcg ccagttcgtc ctacaaacta tgaacagtgg
     6061 attttcaacc aaatttttca ggaacgcgtc cattttgatt gcgctgtttt ttgcaatcga
     6121 agccgggcgc gtccatgcgc aaaaccaaaa ttacagcagc cggattgatt acgatgtcct
     6181 caaacgcgag cagcgggggt tggtaaacgt cttaaccgat ggtgaaaccg acctgagcag
     6241 cggcgaaacg cggctgcgca acaattcgct gcgtctttcc gctgaagaag tcgctcgcgc
     6301 cgcatccaaa gtggcgcact tgcgcgccat ggcgcaattg cacccggaac gttgcgcgcg
     6361 cacaaccggc gctttcctgc ttcccgccgg cgccgccctg caatcgctga ccaataactt
     6421 cttcgggaac atcaaaccgc gcatcgtccg cctcgaagac aacctggatg cttatgtcac
     6481 ccagcttgaa cggctggacc ccaaattggc ggcggaattg cgctccttga ttgacgctta
     6541 taaccgcgcg gccttcgaca tcgtcaacaa tgccgcaaac gggtccggct cgatcgaata
     6601 caaccccagt tcgattgatg ccgcagtcgc gccgaacctc cgagggaata aaatttttga
     6661 aaatataaac aggtctggcg gcggaggcac gtcgggcggc ggtgctggcg atcccaatga
     6721 cccgaacaat ccgaacagcc ccaattatcg tggcaatgga gcgaacgatc ccaacaaccc
     6781 gaattatagg ggtggcaacg gtgcgaacgg cggaaatggc gtcatcaatc caggctcctt
     6841 ccacggcacc atccgcgaca ccggcgggca aatcctgaac gcggacgatt tcctggacaa
     6901 caaaacggtc gtgaaacgga caaaaagcga caacggcatt ttgacccgcg aggaaaagac
     6961 gtccggcgac attgtgcctg atggcaacgg cggcgcggtc ttccggaagc gcgattcgaa
     7021 ggcgatcgac tggcagttcg acatcgaggc gaacgaaaat ggtttcgcat tggtggaccg
     7081 ttcgaacggc ccggctgacg aggacaaagg ctttagcgtg caatcgtgga ccgttaaaga
     7141 tccttccggc cagacacagg actttcccgg aacggagcag ttgaagtatg ccatgagcgc
     7201 gccgggcaaa tataccatca ccgccaaggt gctgaccaag ctcaagacca attttacgat
     7261 ttatatcagc ccggtgaaca attgacatcg cgggctgacg ttaagggcag ggtgacctat
     7321 aggcttatag gtttgtagaa cctattagta aaccatcatt gctggctgaa ttgccgccgg
     7381 ttcaatgatt ccgaccaggc acgctgaatt gctgaatctg cctcctgaaa ccagccctcg
     7441 ggaggcacaa ggggattgtc tgcctttgca acaggagttg cttgccatca atcccgcttt
     7501 accgcccccc aaaaaacctc ttagcgggcc ggaggaagtt ctcaatgatg tcagcaacgg
     7561 cgctccgccg gaaactgcgc ccacgccaca atccggcgct gaattttgcg gaacggttcc
     7621 ggttgaattt ctcagcgtat tggacctcga tgaaaatgcg aacgccgagc aggcattggc
     7681 aaaagcccaa gcgcgcctga aagaaacggt ggatgcccaa agctctctca aacccaggca
     7741 tcccgcccat cccaaactca agcgtgaccg cgcgcgtttc gagcagatga ccgccgagtt
     7801 gcgggaaatc gtttttagtg gaaaggtgga cgccctttgc gaccgcaccg aagaagcgct
     7861 cgaatgccgt ccgccgaaca gggcgaaggc gcgtgattgc gttcgacgcg cgcggacgga
     7921 aggaacgcat ctccagcccg agagcgcgtg tctgcggcgt ttgcaaaagt tagaggtgaa
     7981 attggaaggc ggcgatgccg attccgcgac tgcgcctatt tccaatgaga ccgcatccgc
     8041 accggcgtcg gtgcatgctg caccaccttt cgttgcgccc gtggaaaatc catttctgcg
     8101 gttggaaagt ttggtcgata aagggatggc ttgcctggcg caggactatc cagacgtgtc
     8161 gggagcgatt gaaagttccg ccgaggctca agccgaggca gacggcaagg aagttcccag
     8221 taatctcagg agccggatgc agcttttcaa gcgcactttg gagcaggtcc gccgcgaggc
     8281 gatccatgcg cgcgtggatg aatgtttgcg acaagccgcc gctgcgatgg ctcaggaccc
     8341 gccggccacg gacgagacgc gggaaaaact tcacctcgcg gaatcgctgc tcaaggaagc
     8401 gcccgattcg tatttccgaa gcgaggtggc ccggcttgaa cgcgtgatcg aggaacatga
     8461 acattggctg tcaacccgcc gcagcaatga aatggaactt ctggctgaaa cgcgtccagc
     8521 cccgcctcca atcgaagcgc atccgcttcc cgcgaaagac gacgtttacc caacaatgcc
     8581 ttggccgcgc gcacccaaaa aaatttccgt caaagtaaat ccaaaagcgc gcgctcaaat
     8641 attgccgttg ccgcccccgg gcatggcacc gatggccccg gaaatgactc ctcagcctcc
     8701 agttcagccc gtggttccct tgaagccaac gaagcggagc aaggcattgg agattggcgt
     8761 ggcgctgttg ctcataggct tgatcagcgc ggcttgcgcc agttttcgca ttgccgggat
     8821 gttgtccacg ctgatgggac attccggaaa tatccaaagc aatcaacctg tgcagccgtc
     8881 ggaattcttt gctgaaaaac ccgcctcgca aacatccgcg ccgctattct cccggcgacc
     8941 ggagattggc gaattgagtg tctcggtg
//
LOCUS       DASZMM010000550         8487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_809014, whole genome shotgun sequence.
ACCESSION   DASZMM010000550 DASZMM010000000
VERSION     DASZMM010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809014"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..5007
                     /locus_tag="VG754_13190"
     CDS             43..5007
                     /locus_tag="VG754_13190"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HWF20217.1"
                     /translation="MNVTPVVYANLHANNVQVPSNIFSGAAFQLTYAVTNLGSGPATG
                     TWIDAISIGTNDGIGSTSTILALVTNTATIPSDGVYSNTVSVVAPQCVSGPYFVCATP
                     DAYFQVNNQLCSPNASECAPTQIQPSYPDLQFLVINKPASAIGGEPFNVSWTVINSGN
                     ATATGSWVDILYVSHYNYLTPYSATPVATNFVNGPLAPGATYTEMASVTLPPTAAGNY
                     YALFQTDASNNVQECIGESNNVAPSFSPISVLPSLYPALAITSVNLPATGVAGQPITV
                     SWTVQNIGTASTGGTFWYDSVYLSLDQVLDLTDIDLNAFNRPFPLAPGQSYTNTQQVD
                     LPPSAGGQYYLIIKVDDGNGLNEYGATAGNLYVPNTPIQISLAVPADLSMQGVTVSPT
                     NALSGQPVTINWTVQNLSSNTATGSWQDGLFLSTNFSFDYSAVLVATKTHSGIVPSQG
                     SYSDSDTFSLPGLSPAIYHAIVRTDIRNNLRETNLTDKTGISTGTITVDVPQLTLGVP
                     TTNTLNNGSQIFYKVLVDSNQTLTIKLTSPSVKDANELYVRYGQMPDLGTYDYAYSQP
                     LSANQEVVVPTTQAGYYYILIHGASVPDAPAPMTIEADVVPFSITSVSPTFLGNQGQV
                     TLTINGAGFDPGAIVSLASGTNTIFASNVMVLNSSEIKAMFFFQSPLLGPYDVVVSNT
                     DPGQVMLPGGVMMQLPTFFNVVVTSSGTTAPRAGGIWDVPCAVYNAGNVDAPYVTVFV
                     GFNSPVQMGFARPANSFPLQSNYPGVDWLNNSATARSADGISLDAFWLRDLEPGQTVN
                     FLWRVVSVPAGPFSYDVSVRSRSADDMAAFLGTYVEGMRLYCLQTNVPGITSDELALA
                     NDGPGWHDHYKSAYVASGLMTGNENGWTNVSGDFLNTCDNDFCWVRAVQLGSVAQMAC
                     GIAAGSGPCAVANTASDTALIWLCRFVLCPPGSSVSGNGNGSSGGSSSSGSSSSGSSS
                     SGSSSSGSSSSGSSSSGSSSSGSSSSGSSSSGSSSSGSSSSGSSSSGSSSSGSSSSGS
                     SSSGSSSSGSSSSSGSGGGDGPGGGHGGSNCDPNDKTGPLGYGAAHYVTAANPMPYTV
                     YFENLPTATSTTREVTVVDPFDTNLDIRTFRLGQIVFSHYTVPVPANRSYFTTRVALS
                     DQASNIVADISAGIDVNTRAATWTLTAIDLNTGLEPLDPNLGLLPPDTTNAVGEGYVT
                     YTIQPVGSAATGTVITNQAIITFESNAPIATPITTNTLDSVAPTSSVAPLPSVVLDTN
                     FTVSWSGSDDANGTGIANYRIFAADNSGGYSVWYNTATNTNNVIVTSASYTGQPGHTY
                     KFYSMASDFAGNVEAPHTNADTTVLVSSNHPPVVPPVPDQVANVGTGLNITNTISDAG
                     ASGSQIGVSLVGAPPGVSASVTGSNIVIHWVPNSFQAGTTNIVQLVITNNGVPPLTNT
                     QTFLVSIPDYVQAGIGLTATRAGDAVCLPMNLFTSTGLTNVQFVVTVPATGFTNWSIT
                     LLSPALCGGTVSSLNATQLLVNLTTCSGSSLLVTNQPIAELCLNVGSEQPSQFVPIPI
                     TAVQAFRANSSQVSNTGGQNGRLVVVADKPLLDIQGGGTNDVFLTLYGIPNTTNTLLA
                     TSALGGTNTFQPVWQSLITNLINPLNISKGTNSQMFFRVVTPSQ"
     gene            5081..6442
                     /locus_tag="VG754_13195"
     CDS             5081..6442
                     /locus_tag="VG754_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase"
                     /protein_id="HWF20218.1"
                     /translation="MTPIVPTREYGNKTMPGVRGAKPILPVSATRGWIVALGFAAWLS
                     ALWLVRSLKLGDTVLAMWICIGAFAVVNYCCDVFFLKVHKKESSGLDWKSSRPSFQRT
                     LIKLVGLLASIGFIALIYWLFPEYYKDFFGVNREHPTFYGEYKEMLQRTLPWWLGLAI
                     PYFYFVDARQKDPEDGYYAAGMAVTLRLDKVNGRVLWQHCLSWLVKGYFLALMFTYYA
                     HDTRTFITYDFSPIRNITSVSSFIRSFGVVFDFCYFFIFLSDVALACTGYLLSFRIFD
                     THVRRVEPTFMGWVVALVCYQPFWSNASSWYLNYTSDYVWGVWLRDKPVLYMIWGSII
                     LVLYAIYLWATIMFGTRFSNLTHRGILTNGPYRWTKHPAYIAKNLAFWLTFIPFIVSQ
                     NIGDSIRRCVLLGLLNYVYYLRAKTEEANLSTDPVYAQYSEWMKEHGIFRWLRRPKSA
                     SSG"
     gene            complement(6448..7521)
                     /locus_tag="VG754_13200"
     CDS             complement(6448..7521)
                     /locus_tag="VG754_13200"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWF20219.1"
                     /translation="MNRFIKSLGESGYRQFYLAFGDANLRQKDWALAANSLSGPLLDF
                     VELFLLQEPVPRKRAVPLLGEEGIAELLKCGVILEKSGALVSDAFLLIYCRGHAMFCQ
                     MKPDTFAYFGDDSLALATYQTPAPGGRVLDLCCGPGIQSYIAATYAAKVTGIEIRKET
                     WRIAQLNRRLNDAQRVEFICSSAEDFAGATNEKFDRILFNPPLVPMVPGYKFAFVGNG
                     GADGLDVTRRIVGLYHGKLSDTGALEFIGVELGRKNSPMECAEIKAIARRHGLGGRVH
                     LLSQHPIKPYAPLFESMAFSLARDNRLEIAQARKILTNHFAKLKADAYWLFFASLHKA
                     ETTSAKKVSVVDVSKSFWGHWFV"
     gene            complement(7518..8018)
                     /locus_tag="VG754_13205"
     CDS             complement(7518..8018)
                     /locus_tag="VG754_13205"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWF20220.1"
                     /translation="MTFRPVVSTQLVITTTLALVVLLALGILFLVLQHQTRDRASVHY
                     SFLFAGCLMIFLVAGSFSFRIRSYEINSGNLVVHVGFGKKIFPLNGLQNVSLEERPFA
                     GATKDAAMAGIWSYCGIFSSQKFGTFHAYATTPAQGALLTWADKKVLVTPDDTTRFMQ
                     SLKPAP"
     gene            complement(8041..>8487)
                     /locus_tag="VG754_13210"
     CDS             complement(8041..>8487)
                     /locus_tag="VG754_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20221.1"
                     /translation="LAATFDCRLLGLRPSPEYNQIEERVFESAPPEEESRQITSKVLD
                     YMSDVLEVGGMDAASRLLLSPVCLSAAKIAAQIDFTKIEAGMRRLQKQGKEVSGSDFL
                     GLVHAELKKNTSGKGSAPGLPEYLAMARDPFRRLDVLMDAMEPKSA"
BASE COUNT         1884 a         2361 c         2283 g         1959 t
ORIGIN      
        1 ggctcctgtg aaaccaataa tgccatcggt tcttcgacga ccatgaatgt cacgccggtg
       61 gtctatgcga atctgcacgc gaacaatgtg caagtcccat caaacatttt ttcaggggcg
      121 gcgtttcagt tgacttatgc cgtcactaat ctcggcagcg gccctgccac ggggacgtgg
      181 attgacgcaa tttccatcgg caccaatgat gggattgggt ccacttcgac catcctcgcc
      241 ttggtgacga acacagccac cattccatcc gacggtgttt acagcaatac cgtgagcgtt
      301 gttgcgccgc aatgcgtctc cggcccgtac tttgtatgcg cgacacccga cgcgtatttc
      361 caggtgaaca atcaactatg cagcccgaac gcgagtgaat gcgcgccgac tcaaatccaa
      421 ccaagctatc ctgacctcca attcctggtg ataaacaagc cggcaagcgc aattggcggc
      481 gagccgttca acgtgagttg gacggtcatc aattccggta atgcgaccgc aactggcagc
      541 tgggtggata ttctttatgt ctctcattat aattatttga ctccatacag tgctactccc
      601 gtggcgacga actttgtcaa tggtccattg gcccctggcg cgacctatac tgagatggcg
      661 agtgttacac tgcctcctac agccgctggc aattactatg ctcttttcca gacggacgct
      721 tcgaataacg tccaggaatg cattggtgaa agcaacaacg tggcccccag tttctcacca
      781 atttctgtgt tgccatcact ttatccggct ctggcaatca cgtcggtcaa cctgcctgca
      841 actggtgttg ccggccagcc aatcactgtg agttggacag ttcaaaacat cggcactgcg
      901 tccactggcg gcactttctg gtatgattca gtttaccttt cactggatca ggtgcttgat
      961 ctaacagata tcgacttgaa cgccttcaac cgtccattcc cgctggctcc aggacagagt
     1021 tacaccaata cgcagcaagt ggatttgcct ccatcggctg gcggtcaata ttatttgatc
     1081 atcaaagtcg atgacggcaa tggactgaac gaatacggcg cgacggcagg caatctgtat
     1141 gttcccaaca ctccgataca aatcagtctg gctgtgcctg cggatctgtc catgcagggt
     1201 gtcacggttt cgccaaccaa tgcgctttcc ggccagccgg tcaccatcaa ctggacggtt
     1261 caaaatcttt caagcaacac agcaactggc tcatggcagg acggattgtt cctgtccacc
     1321 aatttctctt tcgattatag cgcggtgctt gtggccacca agacacactc cggcatcgtt
     1381 ccctcgcaag gctcgtactc cgacagcgac actttcagcc tgcccggact cagccctgca
     1441 atctatcacg cgatcgttcg cacggatatc cgcaacaacc ttcgtgaaac caatttgacc
     1501 gacaagaccg gaatctcgac cggcaccatc actgtggacg tgccgcagct tactcttggc
     1561 gttccgacca ccaacactct gaacaacgga agccagatat tctacaaggt gctcgttgat
     1621 tcaaatcaaa cgttgaccat caaactgacc agcccttcgg tcaaggacgc caacgaactt
     1681 tatgtgcgct acggacagat gcctgacctt ggcacttacg attatgccta cagtcagccg
     1741 ctttctgcga accaagaggt cgtcgtgccc accacacagg cgggttatta ttacatcctc
     1801 atccatggcg cgagcgtgcc ggatgcgccg gcaccgatga ccattgaagc cgatgtggtg
     1861 cccttcagca tcaccagcgt cagcccgact ttccttggca accaggggca ggtcacactc
     1921 acgatcaacg gcgccggctt tgatccgggc gccattgtaa gcctcgcttc aggcacaaac
     1981 accatctttg cctcgaacgt gatggtgttg aacagttcgg agatcaaggc catgttcttt
     2041 tttcaatcac ctcttctcgg accgtatgac gtcgtggtga gcaacaccga tcccggccag
     2101 gtgatgctgc ccgggggtgt gatgatgcag ttgccgacct tctttaatgt tgtcgtgact
     2161 tccagcggga ccactgcgcc gcgcgccggt ggaatttggg acgttccctg tgcggtttac
     2221 aacgccggga acgtggacgc tccttatgtg actgtttttg tgggcttcaa ttcaccggta
     2281 caaatgggct tcgcgcgccc ggccaacagc tttccgctgc aaagcaatta tcccggagtg
     2341 gattggctga acaactccgc caccgcccgc tctgccgacg gcatcagcct ggatgcattt
     2401 tggctgcgcg atctggagcc cggccagaca gttaatttct tgtggcgtgt ggtttcggtc
     2461 ccggcaggtc cgttctctta tgacgtttct gtccgttccc ggagcgccga tgacatggcg
     2521 gcattcctgg gcacctatgt tgaaggcatg cgcctgtatt gcctgcaaac caatgttccc
     2581 ggcatcactt cggatgaact ggcattggca aacgatggtc ccggatggca cgaccattat
     2641 aaatccgcat acgttgcaag tggtctgatg accggcaacg aaaacggctg gaccaatgtc
     2701 agtggagatt tcttgaacac ctgcgacaat gatttctgct gggtaagggc ggtgcaactt
     2761 ggatcggttg cccagatggc atgcggcatt gccgcaggtt caggcccatg tgccgtggcc
     2821 aatactgcgt cggacactgc tttgatttgg ctctgcagat ttgtgctctg tccgcctgga
     2881 agcagcgtca gcggcaacgg aaatggttcc agtggcggaa gcagcagcag cggaagcagc
     2941 agcagtggta gcagtagcag tggaagcagt agcagtggta gcagtagtag tggcagcagc
     3001 agcagcggaa gcagtagtag tggaagcagt agtagtggaa gcagcagtag tggaagcagc
     3061 agtagtggaa gcagcagcag tggaagcagc agcagtggta gcagcagcag tggtagcagc
     3121 agcagtggta gcagcagcag tggtagcagt agcagtggta gcagtagcag cagcggaagc
     3181 ggcggcggtg acggtcccgg cggcggtcac ggtggttcca attgtgatcc gaacgacaag
     3241 acgggaccgc ttggatacgg cgcggcgcat tacgttaccg cagcgaaccc aatgccatac
     3301 acggtttatt tcgagaatct gcccacggcg acttcaacga cccgtgaagt gacggttgtg
     3361 gatccgtttg acacgaattt ggatattcgc actttccgtc tcggccagat tgtgttcagc
     3421 cattatacag tgccggtccc ggcaaaccgc agttatttca caacccgcgt ggcgctgtcg
     3481 gaccaggcga gcaacatcgt cgcggacatc agcgcgggaa ttgatgtgaa cacccgcgcg
     3541 gcaacatgga ctttgaccgc gattgatctg aacacaggcc tcgagccgtt ggacccgaat
     3601 ttaggattgc tgccgccgga cacgaccaat gctgttggcg agggttatgt gacttacacg
     3661 attcaaccgg ttggcagcgc ggccaccgga acagtcatca cgaaccaggc gatcatcacg
     3721 ttcgaaagca acgcgcccat tgcgacgccc atcaccacga acacgcttga ttccgtggcc
     3781 ccgacgagca gtgtggcacc gttgccttcg gtggtgttgg acaccaactt caccgtttca
     3841 tggtccggtt cggatgatgc caacggaacc ggcatcgcaa attacaggat atttgcggcg
     3901 gacaattcag gcggatattc ggtgtggtac aacacggcga cgaacacgaa caatgtgatt
     3961 gtcaccagcg cgtcctacac cggccaaccc ggccatacgt ataagtttta cagcatggcc
     4021 agcgactttg ccggcaacgt cgaggcgccg catacaaatg cggacaccac cgttcttgtc
     4081 tccagcaacc atccgccggt cgtgccgcct gttccagatc aagtcgccaa tgtcggcacc
     4141 ggcttgaata ttaccaatac gatcagtgat gccggagcat cgggttcgca aatcggagtc
     4201 agcttggtgg gcgccccgcc gggagtcagc gccagtgtca ccggatcgaa catagtcata
     4261 cattgggttc ccaattcctt ccaggcgggc acaaccaata ttgtgcagtt ggtcatcacc
     4321 aacaacggcg tgccgccatt gaccaacaca cagacgttcc tggtgtccat tccggattat
     4381 gtccaggctg gaatcggatt gacggccacg cgcgcgggcg atgcggtgtg cctgccgatg
     4441 aacctgttca cgagcacggg cttgacgaac gtgcaattcg tcgtgaccgt tccggccaca
     4501 ggattcacca actggagcat cacattgctc tcgccggccc tgtgcggcgg cacggtttcg
     4561 agccttaatg cgacacaatt gctcgtcaac ctgacgacct gctctggttc gtcgctattg
     4621 gtcaccaatc agcccatcgc cgaactgtgc ctgaatgtcg gttcagaaca accgtcgcaa
     4681 tttgttccca tcccgatcac tgctgtgcag gcattcaggg ccaacagttc gcaggtgagc
     4741 aacaccgggg ggcaaaacgg gcgcttggtc gttgttgcgg acaaaccgtt gctcgacatc
     4801 cagggtggcg gcaccaacga cgtgttcctg acgctctatg gaattccgaa cacgacgaac
     4861 accttgctgg caacatctgc gctgggcggc acaaatacgt tccagccagt ctggcagagc
     4921 ttgatcacca atctcataaa tccgctgaac atttccaagg gtaccaacag ccagatgttc
     4981 ttccgcgtcg tgacaccgtc gcagtaattc ctgagaaatt gcgttccttt cgtaatcttg
     5041 cagtgcgaag tggcgctgct aagatggcag gcgtaaatcg gtgacaccta tcgttccaac
     5101 ccgggaatac gggaacaaaa ccatgccggg cgtgcgcggc gcaaagccca ttctgccggt
     5161 gtccgcgact cgcggttgga ttgtggcgct tggctttgca gcatggttga gcgcgctttg
     5221 gctggtccgt tcgctgaagc ttggcgacac ggtgctcgcg atgtggattt gcatcggtgc
     5281 cttcgccgtt gtgaactatt gctgcgacgt tttctttttg aaggtccaca aaaaggaatc
     5341 aagcggtctg gattggaaat cttcgcgacc ctcttttcaa cgcacgctga tcaaactcgt
     5401 cggcttgctg gcgtcaatcg ggttcatcgc cctcatctat tggcttttcc cggagtatta
     5461 caaagatttc tttggagtga acagggagca tccgacgttt tatggagagt acaaggaaat
     5521 gttgcaaagg acacttccgt ggtggcttgg cctcgcaatt ccttatttct attttgtgga
     5581 tgcacggcaa aaagacccgg aggatggtta ttacgcggcg ggaatggcgg tgactttgcg
     5641 gttggacaaa gtgaacggaa gggtgctgtg gcagcattgc ctgagctggc ttgtgaaagg
     5701 atattttctt gcgctcatgt tcacgtacta tgcccatgac acgcgcacat tcatcacgta
     5761 cgatttttcc cccatccgga atatcacatc cgtttcttct tttatccgaa gtttcggtgt
     5821 tgttttcgat ttttgttact ttttcatctt tctttcggat gtcgcgttgg cttgcaccgg
     5881 ttatctgctg tcctttcgga tttttgacac gcatgtgcgt cgtgtcgaac caacattcat
     5941 gggctgggtc gtggcgctgg tttgttatca accattctgg tcaaacgcct cctcgtggta
     6001 ccttaattat acaagtgatt atgtctgggg cgtctggctc cgggacaaac cggttctata
     6061 catgatttgg ggttccatca ttcttgtcct ttacgcaatc tatctgtggg cgacaatcat
     6121 gttcggcacg cgtttttcga atctgaccca tcgcggaatc ctgacaaacg gtccgtatcg
     6181 ctggacgaaa catccggcct acatagccaa aaaccttgct ttttggctga cgttcatacc
     6241 tttcattgtt tcacaaaaca ttggcgattc catccgccgc tgcgttttgt tggggctgct
     6301 caattatgtt tattatctgc gggcgaaaac agaggaggcc aatctgagca ccgacccggt
     6361 gtatgcgcag tattccgaat ggatgaagga gcatggaatt ttccgctggc tgagacggcc
     6421 caagtccgct tcctccggct gacatttcta cacgaaccaa tgtccccaaa acgatttgga
     6481 gacatccacc acggaaactt ttttggcgct tgtcgtttcg gccttgtgaa gcgaggcaaa
     6541 gaagagccag taagcatcgg ctttcaattt ggcaaaatgg ttcgtcagga tttttcgggc
     6601 ctgcgcaatt tccagtctgt tgtctcgcgc aagactgaaa gccatggact cgaaaagcgg
     6661 cgcgtacggt ttgatgggat gctggctcag gaggtgaacg cgtccgccaa gcccatgccg
     6721 gcgcgcgatg gccttgatct ccgcgcactc catgggactg tttttccgac ccaattccac
     6781 cccgataaat tcaagcgcgc ccgtatccga aagttttcca tggtaaaggc cgactatccg
     6841 gcgtgtgaca tccagtccat ccgcgccgcc attgccgaca aaagcgaact tgtaaccggg
     6901 gaccatcggc accaatggcg ggttgaagag aatgcgatcg aatttctcgt ttgtggcgcc
     6961 ggcaaaatct tccgccgaac tgcaaataaa ttccacccgc tgcgcatcat tgagccggcg
     7021 attcaattgc gcgatgcgcc acgtttcctt ccggatttcg atccccgtga ctttggcggc
     7081 gtatgttgcc gcgatataac tttggatgcc cggaccgcaa caaaggtcca gcacgcggcc
     7141 gccgggggca ggggtttgat acgttgcaag cgccaggctg tcatctccga aataggcaaa
     7201 cgtgtccggc ttcatctggc agaacatcgc gtggccgcgg caataaatga gcagaaaggc
     7261 gtcactgaca agcgctccgg atttttccag aatgacgccg catttgagca attcagcgat
     7321 gccctcttcg ccgagcaagg ggacggcgcg cttgcgcggc accggttcct gcaacaaaaa
     7381 aagctccacg aaatcgagga gcggccccga caaactattt gcggcgagcg cccaatcttt
     7441 ctgacggaga ttggcatcac caaacgcaag atagaactgg cgataaccgg attcgcccaa
     7501 gcttttgata aagcggttca tggggccggt ttcagggatt gcatgaaacg cgttgtgtcg
     7561 tcgggagtga cgagcacttt tttatccgcc caggtaagca gggccccttg cgcgggggtt
     7621 gtggcatagg catgaaaagt cccgaatttt tggctgctga aaatcccgca ataggaccag
     7681 attccggcca tggccgcgtc tttcgtggca cccgcaaaag gccgttcttc aagactgacg
     7741 ttttgcaggc cattcaaggg aaagattttt tttccaaaac cgacatgcac caccagattg
     7801 cccgaattga tttcatagga gcggatgcga aagctgaacg agccggccac cagaaaaatc
     7861 atcaggcagc ctgcaaacag aaaggaataa tgcacggagg cccggtcccg tgtttgatgc
     7921 tggaggacta aaaacaaaat tcccaacgcg agcaaaacca cgagtgccaa tgtcgtggtg
     7981 atgacaagct gggtgctgac aacgggccgg aaagtcatgg ctggctaaaa cttggccacg
     8041 tcacgcggac ttgggttcca tggcgtccat gagaacatcc aacctgcgga acgggtcgcg
     8101 cgccatggcc agatattccg gcaaccccgg agccgagcct ttgcccgagg tatttttctt
     8161 caattccgca tggaccagtc ccaagaaatc gctgccggaa acttctttac cttgcttttg
     8221 caaccggcgc atgcctgctt cgatttttgt gaagtcaatt tgcgccgcaa tctttgccgc
     8281 agaaagacag accggcgaca gcagcaaccg cgaggccgcg tccattccgc caacttccaa
     8341 aacgtcggac atgtaatcga gcactttgga cgtgatttgc ctcgattctt cctccggcgg
     8401 cgcggattcg aaaacgcgtt cctcaatttg attgtattcg ggcgaaggcc gcaaccctag
     8461 cagcctgcaa tcaaaggtcg ccgccag
//
LOCUS       DASZMM010000551         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3540732, whole genome shotgun sequence.
ACCESSION   DASZMM010000551 DASZMM010000000
VERSION     DASZMM010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540732"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1030)
                     /locus_tag="VG754_13215"
     CDS             complement(5..1030)
                     /locus_tag="VG754_13215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF20222.1"
                     /translation="MRSAINPLAGVFPANAGRRKFLQTVGAFGLGAALNATAAAEKTE
                     GDGSAVSVSSNADEVPRRSLGRAGVQISALGCGGHHLGDIKKPDEAIELIHRALDAGI
                     TFFDNCWEYYNGETENILGRGLKGRRDQIFLMTKVCTHGRSAELALRMLEQSLRRLQT
                     DHLDLWQIHAISFDNDPELAYAKDGVIEALDKAKQQGKTRFVGFTGHKNPAFHLEMLK
                     RGFPFDTVQMPLNAFDATFHSFEQQVLPEVNRRGMAALGMKSMGGTADAVKKGVVKAE
                     ELLRYAMSLPVATTISGMDSLKVLEQNLRVARGFKPMTPEEMDALRQRCAPMAADGRF
                     EPYTRLR"
     gene            1213..>1539
                     /locus_tag="VG754_13220"
     CDS             1213..>1539
                     /locus_tag="VG754_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWF20223.1"
                     /translation="MSLKVLIVPDKFKGTLSAPAAAAAIARGWRKARRDDALELLPMS
                     DGGDGFGEVLSGLLGAKKIALKTMDAAHRPIRTHWWWEPKSRTAIIESANVIGLALLP
                     GKQFHPF"
BASE COUNT          333 a          453 c          414 g          339 t
ORIGIN      
        1 aatttcaacg aagccttgtg taaggctcga accgtccatc cgcggccatc ggcgcgcaac
       61 gctggcgcaa ggcgtccatt tcctccggcg tcatcggctt gaatccacgc gcgacacgca
      121 agttttgttc aagcaccttc aacgaatcca tgccgctgat ggtcgttgcg accggaaggc
      181 tcatcgcgta acgcaacaat tcctccgctt tgacgacgcc ctttttcacc gcgtcggcag
      241 tgccgcccat gctcttcatt cccaacgccg ccatgccgcg acgattcact tccggcaaaa
      301 cctgctgctc gaaactgtgg aacgtggcgt cgaacgcatt caagggcatc tgcaccgtgt
      361 cgaacggaaa accgcgcttg agcatttcga gatggaacgc gggatttttg tggccggtga
      421 aaccgacgaa gcgggttttg ccctgttgct tggccttgtc gagcgcctcg atgacgccgt
      481 ctttcgcata agccagttcg ggatcgttgt cgaaactgat ggcgtggatt tgccaaaggt
      541 caagatggtc ggtctgaagg cggcgcaacg attgttcgag catccgcaag gcaagctcgg
      601 cgctgcgtcc gtgcgtgcaa accttggtca tcagaaaaat ttggtcgcgc cggcccttga
      661 gtccgcggcc cagaatattt tccgtctcgc cgttgtaata ttcccagcaa ttgtcgaaaa
      721 aagtgatgcc cgcatcgagc gcgcgatgaa tgagttcaat ggcctcgtcc ggcttcttga
      781 tgtccccgag gtgatgacca ccgcaaccaa gcgcggaaat ctgcacaccg gcgcgtccca
      841 aagagcgtcg aggaacttcg tccgcattcg atgaaactga aaccgccgag ccatcaccct
      901 cggttttttc agccgctgca gtcgcgttca acgccgcgcc caatccaaac gcgccgacgg
      961 tttgcagaaa ttttcttcgt ccggcatttg ctggaaaaac gcccgccaaa ggattgattg
     1021 cactacgcat aaatgttccg cctttctgct gtccgaagtg ttccatctaa acagggccaa
     1081 aaacttcctg tcgcgcctat aaagaaaata tgcttttcat gcgtgttgct gtaataggga
     1141 gaattaccac tggacgggag tgacaaacct tttcgcattg cgcatcgaac gcgccggact
     1201 gataaatcgg gcatgtcatt gaaggtcctg atcgtgcccg acaaattcaa gggcactctt
     1261 tccgctcccg ccgcggcggc tgccatcgcg cgcggttggc gaaaggcgcg tcgtgatgat
     1321 gcgctcgaat tgctcccgat gagcgatggc ggtgatggct tcggcgaagt tctcagcgga
     1381 ttgctgggcg caaaaaaaat cgcgctcaaa accatggacg cagcgcatcg ccccatccgg
     1441 acacattggt ggtgggagcc gaaaagccgc actgccatca tcgaatccgc gaatgtcatc
     1501 ggtctcgcct tgttgcccgg aaaacaattt catccgttc
//
LOCUS       DASZMM010000552         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4642051, whole genome shotgun sequence.
ACCESSION   DASZMM010000552 DASZMM010000000
VERSION     DASZMM010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4642051"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1840
                     /locus_tag="VG754_13225"
     CDS             <1..>1840
                     /locus_tag="VG754_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HWF20224.1"
                     /translation="DAIYFGLERFNARMRAHNFTEADLPRLMEFLHRRGVKGYVTFNT
                     LVFANEMAEAEQYLRAIIAAGVDAAIVQDVGICRLIRKLSPDFPIHASTQMTISSAGG
                     VEFARELGCDLVVLARECSLKEIEKIRNAQFDSAVPLEVFVHGALCVAYSGQCLTSEA
                     LGGRSANRGECAQACRMPYELISDGKPVPLGDKRYLLSPQDLAGIEVLPELVRSGVAS
                     LKIEGRLKTPEYVANITRVYRKALDAAMGGVAPDTVGKNAAQYDLEMGFSRGLYTGWF
                     RGNNNQELVHARFGKKRGVYLGEVARVQGERIALQLHAPLKPGDGVVFDAGHPDQDEE
                     GGRVYTLEMRGKETLVGFGHGDLNFSRIQPGDKLWKTSDPELDRRLRQTFAGDKPQFQ
                     RPITMEVHGSVGQSMTLITRDEEGHIAQADSTMPLAIAEKQPLGIEKLREQLGRLGGT
                     PFELGELKNLLNGAVMLPVSELNRLRREVVSQLETLRAAPKRWTVVAGNVSFQTTANQ
                     NAKEAENSRFQLREKRETNAELIVLVRNLPQLETALECGVQTLYCDFENPKKYREAVA
                     TAHCASGCAAPQRTIWVAPPRIFKMGEEWILDQVRSCNADGYLVRNY"
BASE COUNT          410 a          507 c          553 g          370 t
ORIGIN      
        1 gatgccattt acttcggctt ggaacgcttc aacgcgcgga tgcgggcgca caatttcacc
       61 gaggccgatt tgccgcggct gatggaattt ctgcaccggc gcggcgtaaa aggctacgtg
      121 actttcaaca cgctcgtctt cgccaacgag atggccgaag cggaacaata tctccgcgca
      181 atcatcgcgg cgggcgtgga tgcagccatc gtgcaggatg tcggcatttg ccggctcatc
      241 cgcaaactgt cgcccgattt tccgatccac gcctcgacgc agatgaccat cagcagcgcg
      301 ggcggtgtgg agttcgcccg cgaattgggc tgcgacctcg tcgtgctcgc ccgcgaatgt
      361 tccctgaaag aaatcgagaa aatccggaac gctcaattcg attccgcagt tcctttggag
      421 gtttttgtgc atggcgcgct ctgcgtcgct tattccggcc aatgcctcac cagcgaagcg
      481 ctcggcgggc gttcggccaa tcgcggcgaa tgcgcgcaag cctgccggat gccatacgaa
      541 ctgatttccg acggcaagcc ggttccgctt ggcgacaaac gttacctgct cagtccgcag
      601 gaccttgccg gaatcgaagt gctgcccgaa ttggtgcgtt cgggagttgc ctcgctgaaa
      661 atcgaaggcc ggctcaagac gcctgagtat gtcgcaaaca taacgcgggt ttatcgcaag
      721 gcgttggatg cggccatggg cggggttgcg ccggacacgg tcggaaaaaa cgcggcacaa
      781 tatgatttgg agatgggatt ttcgcgcggg ctttacaccg gatggtttcg cggcaacaac
      841 aaccaggaat tggtccacgc gcgtttcggc aaaaaacgcg gcgtttatct tggcgaagtc
      901 gcgcgcgttc aaggcgaacg catcgccttg caacttcacg ccccgctcaa gcccggcgac
      961 ggcgtggtct ttgatgccgg tcatcctgac caggacgagg aaggcgggcg cgtttacact
     1021 ttggaaatgc gcggcaagga aaccctggtc ggtttcggcc atggcgattt gaatttcagc
     1081 cgcattcaac ccggcgacaa attatggaag acgagcgacc cggaattgga ccgccgtttg
     1141 cgccaaactt ttgcgggaga caaaccgcag ttccaacggc caatcacgat ggaagtccat
     1201 ggcagcgttg gccaatcgat gacgctcatc acgcgcgatg aggaggggca cattgcgcag
     1261 gcggattcga caatgccgtt ggctatcgcc gaaaagcagc cgctgggcat cgaaaaatta
     1321 cgcgaacaac ttggccggtt gggcggaacg ccgtttgaac tgggcgaatt gaaaaattta
     1381 ctgaatggcg cggtcatgct gccggtgagc gaattgaatc gcctgcgccg cgaggtggtg
     1441 agccagttgg aaactttacg cgccgcacca aagcgatgga cagttgtagc tggaaacgtg
     1501 agttttcaga ccaccgcaaa tcaaaatgca aaagaggctg aaaactcacg ttttcagcta
     1561 cgggaaaaac gtgaaaccaa tgccgaattg attgtcctcg ttcgcaactt gccgcaactg
     1621 gaaacggcgc ttgagtgcgg cgtgcaaact ctttattgcg attttgagaa tcccaaaaaa
     1681 taccgcgaag ccgtcgcgac ggcccattgc gcgagcggat gcgccgcgcc gcaacgcacg
     1741 atttgggtcg cgccgccgcg cattttcaag atgggcgagg aatggatctt ggatcaagtt
     1801 cgctcgtgca atgccgacgg ctatcttgtc cggaattacg
//
LOCUS       DASZMM010000553        12614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2997729, whole genome shotgun sequence.
ACCESSION   DASZMM010000553 DASZMM010000000
VERSION     DASZMM010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12614
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997729"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1633)
                     /locus_tag="VG754_13230"
     CDS             complement(14..1633)
                     /locus_tag="VG754_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20225.1"
                     /translation="MAESGNAAPVAAPDQPAVPQGKLGRLIWGLRTNSRFAFLLQVGS
                     RILTAALGLLWTRVLTDAMGKALIGLFYALQDVFSLGGLGDLGVGGAIGIRTGQYLSH
                     GKSKEPELLNFLASARTVFIVLIIASGCGVLILSPWIPHWKNFAVVSDAGSLIKVFQV
                     GAVMVAGFMMFSYFSNVNYACGNIAWPVLPDFILLQITLACHLFLARQQQPLWVQFLP
                     YMCATFIKLFLMRFYVRNSHPPLSRIWPLTFDWKLAMNLFEGSFWVYLCCLGNAIYRK
                     TDNLVITAGFTPGVNTEYNFNYKACDLMFMVVTTASYVILPKVTQWMASTDPKDHERV
                     VLEMKRLNQFQTLLGCGAALAYLAFNNIFIRLWWYHRENLIRPAALTLQIAFALNMAV
                     TASGDTALQLALRSGKNGLRVVGVLIGLTGLLNVALSLTAMQKHWLEGIALATVLAQS
                     VLMLGSSFFLCGHLKVPRLPWMFKGCALPLAGIGFAGWLRTLWPMDSLQHVLMLLGAY
                     AALFVLAAWGLGINRALIKNEFQLIRKLVGK"
     gene            complement(1626..2576)
                     /locus_tag="VG754_13235"
     CDS             complement(1626..2576)
                     /locus_tag="VG754_13235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF20226.1"
                     /translation="MRYQLYKLFNAFVRVIPIPILRRMLRTMREFPDIADRVGYTVYP
                     QVFYNPFPEPQQVDLVKLKKKRNLPGVNFNMDETNRLMGEFLKSAAEIQGFIKNRPAN
                     TLKIWDDTYPVCDSAILYAMLRHLKPRNYIEVGCGWSSRCSAAALERNQLEGHPCNAS
                     FIEPYPPPHLIEVKLPGEFLKQKIEQTPLEFFLRLEAGDVLFIDTSHVIKTQNDVEYE
                     FIQILPALKSGVIVHIHDICSPYDYPAEWLVGTGPNRGGNNEQYALECLLSGGGDWEV
                     IFPVYLLWKDHRAKLAQLIDSERWPAAFWMRKLKTHQANG"
     gene            complement(2673..4283)
                     /gene="lnt"
                     /locus_tag="VG754_13240"
     CDS             complement(2673..4283)
                     /gene="lnt"
                     /locus_tag="VG754_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWF20227.1"
                     /translation="MAGLLLAASFPKISIAGLAWVAPGLILLTAIGKPGRQTFRIGYV
                     AGLAHHLASLYWLLMIPVAWFPILGWVALSAFLALEMGAWVWLSWKFFPAKLDGHTVE
                     NFAAQFFSVPWTRRIVWAISCAAIWVALEMYQARILGGFPWNLLGISQYKLIPLIQVA
                     SATGIYGVSFLLVWMSLSLLSAGFRIIRRPALRSAWVGEIILPALAVSALYAIGCHKV
                     LKPTPRSPELTVALVQPSIPQTLIWDTNENAARFQQLLKLSEEALKQKPDLMIWPEGA
                     IPEMVRYDEETHRAVTSLARAHKVWLIIGSDDAEPHPNAGPQDEPDFFNSSFLVSSSG
                     QLVERYKKRNLVMFGEYIPLFRWLPFLKYVTPIKGGFTPGEGAVPFELKDLNVKISVL
                     ICFEDTFPQLVREYVSDDTDFLVNLTNDGWFSQSSAQWQHAAAAIFRAVENGVPLVRC
                     SNNGLTGWVDSCGRLREMFQSPANGIYGQGFLIAHVPVLNPGETRTPTFYRLHGDVFG
                     WGCAALVVLQMLKFCIFKRKTNSAPANS"
     gene            complement(4369..5754)
                     /locus_tag="VG754_13245"
     CDS             complement(4369..5754)
                     /locus_tag="VG754_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF20228.1"
                     /translation="MKSTLSRLFASPAILIGTSVAVSLFCAFAVQAATSMEENWPQWR
                     GPFANGVAPAANPPTSWSETSNIKWKVKLPGSGSATPIVWGDKAFVETAVPTGKKPEA
                     SAKADSENTAPGQGGRQGGMGGEKPDEIFQFMVVCLDRETGKTLWQKVAREEVPHEGV
                     RPREGSFAASSPMTDGRHVYGYFGSRGLYCYDMDGKLEWQKDFGKLHIKMGFGEGSSA
                     ALSGNTIIVNWDNEGGSFIVALDKNTGKELWRESRDEQTTWATPLVVQHDGKAQVITD
                     GSKKIRSYDLDSGKVLWECSGLTANVIPSPVADDNTVYAMSGFRGNALVAIRLGHAGD
                     LTGTDAIAWHREKGTPYVPSPMLYGSRLYYFSNNNGMISCVDTKTGNVLIDAERLEDL
                     KNVYASPVGASGRVYLVGRNGVTVVIKDSDKLEVLATNKLDESFDASPAIAGRELFLR
                     GHEYMYCIAGK"
     gene            5926..7476
                     /locus_tag="VG754_13250"
     CDS             5926..7476
                     /locus_tag="VG754_13250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HWF20229.1"
                     /translation="MNRTFALGLLFALMMAAALRLPQLGARPMHNDEGVNAIKLRGLW
                     GRGSYQYDPQEYHGPTLPYISWVWMKLTGPKNFTSFDEAQLRTITVLFGLGLIFLIPL
                     IADGLGKRAAICAAVMTAISPAMVFYSRYYIHEMLLVFFTFLALAAGWRYFRSRKIGW
                     AILTGAAVGLMQSTKETFLLAIAAMAVALIAEHLWSGVGRMENTERPFKWNFKHIAIA
                     LGVWVVVMMILFSSFFTNWSGPMDSVLTYFPWLHRAGGASPHIHPWNFYLERLAWFHV
                     RKGPVWSEGLILFLALIGLVAAFIRKGITEGDRSFVRFVAFYTILLTVAYCAIGYKTP
                     WCLLSFWQGMILLAGVGTVSLLHWLRKRPLQIVAGVLLLVGGGQLACQAWRANVEYAA
                     DPRNPYVYAQTSPDATNLVALVKALGDVSPQRQHMLIKVMAPENAYGPLPWYLRDFDQ
                     TGWWSELPPDPYAPVMIVSTKFNAALDEKKTHLMAGLFELRPSAWFEVYVETNLWHDY
                     LETKGAKK"
     gene            complement(7640..9103)
                     /locus_tag="VG754_13255"
     CDS             complement(7640..9103)
                     /locus_tag="VG754_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF20230.1"
                     /translation="MKKTSTDSKISRRHFLAATGAALALPTFIPASALGRNGRPAPSE
                     RVILGVAGWGMQGPGNTKHFLGISECQVVAACDLDEGRLHRNDNSAVKTINNHYKNKD
                     CKAYHDYREMMARSDIDAVMLAVPDHWHALVATEAAHRKKDIYGEKPLARTIAEQQAI
                     VHAVEKNGRIWQTGSWQRSEANFHKGAEIVRNGLIGRVTHVEVGLPAGHSDFPHTAPD
                     LLKKFASKPTDNDYLTKVVPGTPDWDAAISQPPAGFDYERWIGPSRMEPYIGARVYQN
                     WRWNYNTGGGQLLDWIGHHCDIAHWGLDFDRSGPSEIEGHGEFPPANAIWNTTGKYRI
                     ELQYPNNIKMTIAGGYPEIKSGTKWIGTDGWVYVDRGKFEASNEDWVNTRTLPENLRK
                     IKLYESNDHWKNFIDCVKSRKPTIAPVQTAHHSAIPGHLGLISMLVGRKLRWDVASER
                     ILDDAEATKLTSRPYRSPWKFPGAHEIYQSPKAVANS"
     gene            complement(9331..9786)
                     /locus_tag="VG754_13260"
     CDS             complement(9331..9786)
                     /locus_tag="VG754_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20231.1"
                     /translation="MTDNDHFGFDPSAALFGPNELSLDLFGYSATKDKGGGDHQAWGP
                     GVAVNYFFTENLGVGAEGYADAFETPYLLNALGEFRYPVPGVSWFAPHVFLGLGREWA
                     NAPQWMGHFGGGAELRFNANTGLLIDVRRVFCVNSPDYTLVRFGFRITF"
     gene            complement(10037..10606)
                     /locus_tag="VG754_13265"
     CDS             complement(10037..10606)
                     /locus_tag="VG754_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWF20232.1"
                     /translation="MKAKSIIVLALALALPASVLLAQNQNDGPPGGGPGPGHRHHPPM
                     PLIQALDTNHDGIISADEIANASAALLTLDKNGDGQLTKDEYLPPRPNRNGDTNNPPP
                     DAQGDSNQARPHRPIPPIVAALDTNGDGIISADEIANAPASLMKLDKNGDGQLTMDEL
                     MGPRPPHGEGPGPSGPGDGQRPPPPEQNN"
     gene            10813..11571
                     /locus_tag="VG754_13270"
     CDS             10813..11571
                     /locus_tag="VG754_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF20233.1"
                     /translation="MNNQTQPSNPSNAAPGRTRVLVIDDDRKLCRLIRDYLEPMGYEI
                     EMSHSGPEGLEKAQGQKFSAVILDLMLPGMDGFEVLKKLRHTSDVPVLMLTGRGEEPD
                     RIVGLELGADDYLPKTFSTRELLARLRAVTRRAARKSSGMPETAEEIVIGPLRVRPES
                     RVALLADQPLVLTPVEFDLLACLARGKGRVKTREQLLDAISDRNYDAFDRSIDVHVSA
                     LRKKLGDDPKNPRFIKTLRVAGYMLIDPESEVAQ"
     gene            11568..>12614
                     /locus_tag="VG754_13275"
     CDS             11568..>12614
                     /locus_tag="VG754_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF20234.1"
                     /translation="MKIRFPLYAKILFWFFLNVVFLCAVFFIVARGQFHFWMDALISG
                     PAGGRMQAVTHLIAEELRDRPQTEWNGVLQRFSSAYNVEFTVFRNDGRQLAGETVTLP
                     PQVRIRLAERHGMNPPPPHGEIEGGHFNPPPGDEMHAPPAPDRPQKIMMHTSAPSRYW
                     VMVHLLMPSQDASPHPTPMVLLAESESLSGGGLFFDLTNLAAGGLAVVLVSVLFWFPL
                     VRGITSSISRMTMATEQIAEGRFHVRTQVRRQDELGSLSEAINRMAERLSGFVAGQKR
                     FLGDISHELCSPIARIQVALGILEQRADEKQKAYLADLREEVEQMSGLVNELLSFSKA
                     SLGGAEIKLQPVALR"
BASE COUNT         2902 a         3342 c         3550 g         2820 t
ORIGIN      
        1 ccaaatgccc cgtttatttg ccaacaagct tgcgaatgag ctggaattcg ttcttgatca
       61 gcgctcggtt tatcccgagt ccccaagctg cgagaacaaa caaggcggca taagctccca
      121 gcagcatcaa cacatgctgc aacgaatcca tcggccacaa agtcctgagc cagccggcaa
      181 aaccgatccc ggccaacggc aatgcacatc ccttgaacat ccagggcaac cttggcacct
      241 tcaaatggcc gcaaagaaaa aagctggacc cgagcatcaa gaccgattgg gccagcaccg
      301 tggccagcgc gatgccttcg agccagtgct tctgcatcgc ggtcagggac agggcgacgt
      361 tcaacagacc ggtcaggccg atcaaaacgc ccacaacgcg caggccgttc tttcccgaac
      421 gaagcgcaag ttgcagcgcg gtgtccccac tggcagtgac ggccatgttc aatgcaaatg
      481 cgatctgcaa tgtcaatgcg gcgggacgaa tcaagttttc ccggtgatac caccaaagcc
      541 ggatgaagat gttgttgaat gccaggtaag cgagcgcggc gccgcagccc aacagtgttt
      601 ggaactgatt cagccgtttc atttccagca ccacgcgctc gtggtctttg ggatcggtcg
      661 aggccatcca ctgggtcact tttgggagaa tgacgtagct tgccgtggtc acgaccatga
      721 acatgagatc gcaggctttg taattgaaat tgtactcggt gttgacgccc ggagtgaacc
      781 cggctgtgat gaccagattg tcggtcttgc gatagatggc atttccgagg cagcagagat
      841 agacccaaaa acttccttca aaaaggttca tcgccagctt ccagtcaaaa gtgagcggcc
      901 aaatgcggct caacggcggg tgggaattcc gcacgtaaaa ccgcatcaaa aaaagtttga
      961 tgaaagtcgc gcacatgtag ggcaggaatt ggacccacaa aggttgctgt tgccgcgcga
     1021 ggaacaaatg gcaggcaagc gtgatttgca gcaggataaa gtccggcagt accggccagg
     1081 caatgtttcc gcaggcgtag ttcacgttgc tgaaatagct gaacatcatg aaaccggcca
     1141 ccatcaccgc gcccacttga aaaactttga tcaaggaacc cgcgtcggac accaccgcaa
     1201 aatttttcca atgcgggatc cacggagaaa gtataagcac gccgcaaccg gatgcgatta
     1261 taagcacgat gaacaccgtt cgggccgatg cgagaaaatt gagcaattcc ggctccttcg
     1321 attttccatg gctgagatat tgtccggtgc ggatgccaat ggctcctccc actccgaggt
     1381 ctcccaaccc gcccagcgaa aagacgtctt gaagcgcgta gaaaaggccg atgagagcct
     1441 tgcccatcgc gtccgtcaac acacgcgtcc acaacaagcc cagggccgct gtaagaattc
     1501 gggagccgac ttgaagcagg aacgcgaaac gcgaattggt ccgcagcccc caaatcagac
     1561 gccccaattt tccttgcgga actgcgggtt ggtctggtgc cgcaacaggt gccgcgttgc
     1621 cgctctcagc catttgcttg atgggttttt aatttgcgca tccaaaatgc ggcgggccat
     1681 cgctctgaat ctatcaactg cgccagtttt gcgcgatggt ctttccaaag cagatacacg
     1741 ggaaatatga cttcccaatc gccgccgcca ctaagaaggc attccaaggc atattgctcg
     1801 ttgttgccgc cgcggtttgg gcccgtgccg accagccact cggcaggata atcgtacgga
     1861 ctgcagatgt cgtggatgtg cacaatgaca ccggatttta aagcaggcaa aatttggatg
     1921 aattcgtatt caacatcgtt ctgggtcttg atgacatggc tggtgtcaat gaacagaacg
     1981 tctccagcct cgagtcgtag aaagaattcc agtggagttt gttcaatctt ttgtttgagg
     2041 aattcacccg gcagcttcac ctcaatcagg tggggcgggg gataaggttc gataaaggag
     2101 gcattgcaag gatgaccttc aagctggtta cgctcaagcg ccgcggcact acagcgcgaa
     2161 ctccagccgc aaccgacctc aatgtaattg cgcggtttca gatgccgcag catcgcatat
     2221 aaaatcgcgg agtcgcagac gggatatgta tcgtcccaaa tctttaacgt gttggccgga
     2281 cgatttttga tgaacccttg tatttccgcg gcggatttca gaaactcgcc catcaagcgg
     2341 ttcgtttcat ccatgttgaa gtttacgccg ggcaaattgc gctttttctt tagcttcaca
     2401 aggtcaacct gctgcggttc agggaaagga ttgtaaaaga cctgcgggta aacggtgtaa
     2461 cccacccggt cggcgatgtc cgggaattca cgcattgtcc gcagcattcg tcgcaaaatg
     2521 ggtatcggaa tcacgcggac aaaggcgttg aataatttat ataactgata acgcataaca
     2581 aacggtcgga atgctaaata cgagtatata acggcgaatg aatgagaaca aggctctccc
     2641 gctttcgcaa gaaattagaa gaaatgtttt cgtcacgaat ttgctggcgc gctgttcgtt
     2701 ttgcgcttga aaatacagaa cttcagcatt tgcaaaacta caagtgcggc gcagccccag
     2761 ccgaaaacat ccccgtgcag ccggtaaaaa gtgggcgtgc gagtttctcc aggattcaaa
     2821 accggaacat gcgcgatgag gaagccctgt ccgtaaattc cgttcgcggg actttgaaac
     2881 atttcgcgca accgtccgca ggaatccacc catccggtca gtccattgtt cgagcagcgc
     2941 acgagcggaa caccattttc caccgcgcga aaaattgcgg cagcggcgtg ctgccattgg
     3001 gccgagcttt ggctgaacca tccgtcattc gtcaggttca cgagaaaatc ggtgtcgtcg
     3061 gaaacatatt cgcgcaccaa ttgcgggaag gtgtcttcga agcagatgag cacggaaatc
     3121 ttcacattca aatctttcag ttcaaatggc accgcgcctt cgcccggcgt gaatccgccc
     3181 ttgatcggcg tgacgtattt cagaaacggc aaccagcgga acaggggaat gtattcgccg
     3241 aacatgacca gattgcgctt cttgtaacgc tcaaccaatt ggccggagga actgacaaga
     3301 aaactgctgt tgaaaaaatc aggttcgtcc tgcgggcccg catttgggtg cggctcggca
     3361 tcgtcggaac cgatgatcag ccatacttta tgcgcccggg cgaggctggt caccgcgcga
     3421 tgcgtttctt catcgtaacg caccatttcg gggattgcac cttcgggcca gatcatcaaa
     3481 tcgggttttt gcttcagggc ttcttcggaa agtttgagca gttgttgaaa gcgtgccgcg
     3541 ttttcattcg tatcccaaat aagggtttgc ggaatgctgg gctgaaccaa tgcgacggtg
     3601 agttcagggc ttcgtggcgt tggcttcaag actttgtgac aaccgatagc gtaaagagca
     3661 gatactgcaa gcgctggcaa aatgatttct ccaacccatg ccgaacgcaa tgcaggccgg
     3721 cgaatgatgc ggaagccagc cgagagcaac gaaagcgaca tccataccag aagaaacgat
     3781 actccataga ttccggtggc cgacgcgact tgaatcagcg gaatcaattt atattgcgag
     3841 atgccgagca agttccaggg aaagccgccg agtatccgcg cttggtacat ttccagagcg
     3901 acccaaattg cggcgcatga gattgcccaa actatcctac gcgtccatgg cacggaaaag
     3961 aattgagcag cgaaattttc aacggtgtgg ccgtcgagtt tggcaggaaa aaatttccac
     4021 gaaagccaga cccatgcgcc catttcgagc gcgaggaagg cgcttaacgc gacccagccc
     4081 agaatgggaa accatgccac cggtatcatc aaaagccaat aaagcgacgc aagatggtgc
     4141 gccaatcccg ccacgtagcc gatgcggaat gtttgtcggc ccggtttgcc aatggccgtg
     4201 agcaaaatca atcccggcgc aacccaggca agacctgcga tgctgatctt gggaaacgaa
     4261 gccgccagca gaagccccgc cacgacggcc catggatacc gactttttat gaacgaacgc
     4321 aatggttgca aagcttgccc caaagttcag caaaggcaag cgccggtgtc atttcccggc
     4381 aatgcaatac atgtattcgt gaccgcgcaa aaacaattcc ctgccggcaa tcgcaggcga
     4441 ggcgtcgaac gattcgtcga gtttgttcgt cgcaagaact tcgagcttgt cggaatcttt
     4501 gatgacgacc gttacaccgt tgcggccgac gagataaacg cgtccacttg cgccgacggg
     4561 cgaggcatag acatttttca aatcttccag gcgttcggcg tcaatcagca cattgcctgt
     4621 cttcgtatcg acgcaagaaa tcatgccgtt gttgttgctg aaataataaa gccggctgcc
     4681 gtaaagcatg ggcgagggga cataaggcgt gcctttctcg cgatgccacg cgatggcgtc
     4741 ggtgccggtc aaatcgcccg cgtggccaag ccggatggca accaatgcat tgccgcgaaa
     4801 accgctcatg gcataaactg tgttgtcatc agcaaccggg cttggaataa cattggccgt
     4861 caggcccgag cattcccaaa gcaccttgcc ggaatccaaa tcgtagctgc ggatcttttt
     4921 ggaaccgtcg gtgatgacct gcgccttgcc gtcgtgctgc acgacgagcg gcgtggccca
     4981 agtggtttgt tcgtcacgcg actcgcgcca aagttccttg cccgtgttct tgtccaatgc
     5041 aacgatgaac gagccgcctt cattgtccca attcacgatg atggtgttgc cggaaagcgc
     5101 ggccgaactg ccttcgccaa atcccatttt gatatgcaat tttccgaaat ctttctgcca
     5161 ttccaacttg ccgtccatgt catagcagta aagtccgcgc gaaccaaaat aaccgtaaac
     5221 atgccggcca tccgtcatcg gtgaagacgc cgcgaagctg ccttcgcggg gacgcacacc
     5281 ctcatgcggc acctcctcac gggcgacttt ttgccaaagc gttttgccgg tctcacgatc
     5341 aaggcaaacc accatgaact gaaaaatctc atccggcttt tcgccgccca tcccgccctg
     5401 acgaccacct tggccgggag cggtgttttc gctgtccgcc tttgcggaag cttccggttt
     5461 tttcccggta ggcacggcgg tttcgacgaa ggctttgtca ccccaaacaa tcggcgtggc
     5521 cgagccgctg ccgggtagtt tcactttcca tttgatattg ctggtttcac tccaactggt
     5581 cggtggattt gcggcgggtg cgacaccgtt tgcaaaaggg ccgcgccatt gcggccagtt
     5641 ttcttccatt gatgtggcgg cttgcacggc aaaggcacag aacaacgaaa cagcgaccga
     5701 tgtgccgatg agaattgcgg gagatgcaaa aagtcttgaa agtgttgatt tcatagggac
     5761 tccaaaattg atgcgagttg gctgagaata gcgcaagaag caacgacacg caagccaatc
     5821 ggatgcagat cttccaagcc aaacgtgctt gtcaccgctt cggcttgggt ttaatgaatg
     5881 gttgatgact gaaacccaaa caagaccagc gattttccgt tcgaaatgaa ccggacattt
     5941 gcgctcggcc ttttgttcgc gcttatgatg gccgccgcgt tgcgtttgcc ccaactcggc
     6001 gcgcgcccga tgcacaatga cgaaggcgtc aatgcgatca aactccgcgg cctctgggga
     6061 cgcggctcct accaatatga cccgcaggaa tatcacggcc ctacactccc ctacataagc
     6121 tgggtgtgga tgaaattgac gggcccaaaa aattttacga gcttcgacga agcgcaattg
     6181 cggacaataa ccgtactgtt cggtttggga ttaattttcc tgattccatt gattgcagat
     6241 ggtttgggaa aacgggccgc aatctgcgcc gcagtgatga cggcgatttc gccggcgatg
     6301 gttttttaca gccgctatta catccacgaa atgctgctgg tgttcttcac ttttctggcg
     6361 ctcgcggcgg gatggcgcta tttccgcagc cgaaaaattg gctgggcaat cctgaccggc
     6421 gcggcggttg gattgatgca aagcacgaag gaaacatttc tgctggcgat tgcggcgatg
     6481 gcagtggcgt tgattgccga acacctttgg agcggtgtcg gtcgcatgga aaatactgag
     6541 cggccattca aatggaattt caaacacatc gctattgccc tgggtgtgtg ggtggtggtg
     6601 atgatgatat tattttcctc ctttttcacc aattggagcg ggccgatgga ttccgtgctc
     6661 acctatttcc cgtggcttca tcgcgcgggc ggggcgtcgc cgcacattca cccgtggaat
     6721 ttctatttgg agcggctggc gtggtttcat gtcaggaaag ggccggtgtg gagcgaagga
     6781 ttgattctgt tcctggcgtt gatcggtctg gttgcggcat ttattcgcaa gggcatcacg
     6841 gagggtgaca ggagctttgt gcgtttcgtc gcattttata cgattctcct gacggttgcg
     6901 tactgtgcca ttggctataa aacgccatgg tgtttgttaa gcttctggca gggaatgatc
     6961 ttgctggcgg gcgttggaac ggtgtcactg ctgcattggt tgcgaaaacg acctttgcaa
     7021 atcgtcgcag gcgtcctgct gctggttggc ggcggacaac ttgcctgcca ggcgtggcgg
     7081 gccaatgtgg aatatgcggc tgacccgcgc aatccttacg tgtacgcgca gacttcgccc
     7141 gatgccacga acttggtggc gctggtcaaa gcattgggcg atgtgagtcc gcagaggcag
     7201 catatgctca tcaaggtgat ggcccccgaa aatgcttacg gaccgctgcc atggtatctg
     7261 cgcgatttcg atcaaaccgg ctggtggtct gaattgccgc ccgatcctta cgcgcctgtg
     7321 atgatcgttt cgacgaagtt caatgccgca ctcgacgaaa agaaaacaca cctgatggcg
     7381 gggcttttcg aactgcgtcc atcggcatgg tttgaagtgt acgtggaaac caatttgtgg
     7441 cacgactatt tggaaacgaa gggagcgaag aagtagttcc aactccattt cataaataac
     7501 aaaaatgcgc cgaattaaga cggcgatgaa atgggcaaac atcgaacgat caacatcgaa
     7561 caccgaacgt ccaatggaac tgcagccggt tcgtcattcg acgctcggcg ttcagcgttg
     7621 gatatttttc gcagcaccct cacgaattcg ccacagcctt gggagattga taaatctcat
     7681 gcgcgcccgg gaatttccat ggcgaacgat agggacggct cgtcaatttc gtcgcttcgg
     7741 cgtcatcaag gatgcgttcg ctggcgacgt cccaacggag cttgcggcca accagcatgg
     7801 agatgaggcc aagatgaccg ggaatcgcgg aatgatgcgc ggtctggacg ggagcgatgg
     7861 tcggtttgcg ggacttgacg caatcaatga aattcttcca atgatcgttg gattcgtaga
     7921 gctttatctt gcgcagattt tccggaagcg tgcgggtatt cacccaatct tcgttggagg
     7981 cttcgaactt gccgcggtcc acgtaaaccc atccgtccgt gccgatccat ttcgtgccgc
     8041 tcttgatttc cggatagccg ccagcaatgg tcatcttgat gttgttggga tattgcaact
     8101 cgatgcggta tttgccggtt gtgttccaga tggcgttggc aggaggaaat tcgccgtggc
     8161 cctcgatctc tgaagggccg gagcggtcaa agtccagtcc ccaatgtgcg atgtcgcaat
     8221 gatggccgat ccagtcgagc aactgtccgc cacccgtgtt gtaattccag cgccaattct
     8281 ggtaaacgcg cgcgccgatg taaggctcca tccgtgatgg gccaatccag cgttcgtaat
     8341 cgaaacctgc gggcggttgg gaaatcgcgg cgtcccaatc gggcgtgccg ggaacgacct
     8401 tggtcaagta gtcattgtca gttggcttcg acgcgaattt cttgagcagg tcgggagcgg
     8461 tgtgtggaaa atcgctgtga ccggcgggca agccaacttc aacatgcgtt acgcgcccga
     8521 tcaatccgtt gcggacgatt tccgcgccct tgtgaaaatt cgcttctgaa cgctgccacg
     8581 aaccggtctg ccagatgcgc ccatttttct cgacggcatg aacaatggcc tgttgctcgg
     8641 cgatggtgcg ggcgagtggc ttttcgccgt agatgtcctt cttgcgatgt gctgcttcag
     8701 tggcgacaag cgcgtgccaa tgatccggca cggcgagcat gaccgcgtca atgtccgaac
     8761 gcgccatcat ttcgcgatag tcgtggtatg ccttgcaatc cttgttcttg taatgattgt
     8821 tgatggtttt gaccgctgaa ttgtcattcc tgtgcaaacg gccctcgtcc agatcacaag
     8881 cagcgaccac ctggcattca gagatcccca gaaaatgctt tgtattgccc ggcccctgca
     8941 ttccccaacc ggcgactccc aaaatgactc tttcggaagg ggcgggcctg ccattgcgtc
     9001 caagcgcgct tgcgggaata aaagtcggga gagccagtgc cgcgcccgtg gcagcgagaa
     9061 aatgacggcg ggatattttg gaatcagtcg aggtcttttt cataattagt cacactctgc
     9121 gatcacaagc cggctgagtt tgttgcgcac cggcagtcgc ttccgcaacg cgtcgcaatc
     9181 attgattgga cggcattggt cagtcggtat tcaggacatg actaacatgt aaccaaaacg
     9241 atgtaaactg cttctttcaa aatggccgca aaaaagtaaa ggcgcggcaa gtagattgcc
     9301 aggccttctg tgtgaacatt gattcagtaa tcagaaagtg atacggaagc caaaacgtac
     9361 cagggtatag tccggtgaat tcacgcagaa aacgcgccgc acgtcgataa ggagtccggt
     9421 gttcgcattg aaccgaagtt ccgcgccgcc gccgaaatgg cccatccatt gcggcgcatt
     9481 ggcccattcc cggcccaagc ccagaaacac gtgcggggca aaccatgaca caccgggaac
     9541 gggatagcga aattccccga gcgcattgag cagatagggt gtttcgaaag catcggcata
     9601 accttccgct ccgacgccga ggttttccgt gaagaaataa ttcaccgcca cgcccggtcc
     9661 ccacgcttga tgatcaccgc cgcccttgtc cttcgttgcg ctgtaaccga acaggtcgag
     9721 agaaagttca ttcggaccaa agagcgcggc tgagggatcg aacccaaagt ggtcgttgtc
     9781 agtcattgcg gcttttgccg tgcccaaatt taaaaaaaga atcgctgagg agagtaacgg
     9841 aacaatcttt tttagtttca tgtgaatcta acaatgcgtg ttgttgatgc cctgtgtgcg
     9901 gtcggtgacc gctttaccca cagcttttgc acggacatta tatgactgcc tcaaaaccag
     9961 ccatggggtg tttacccaag acgtaaaacg ccgccggatt gaatcaaccc gacggcgcac
    10021 tgcggggaaa caatgcttaa ttgttctgtt ccggcggagg cgggcgctgg ccatcgccag
    10081 gaccacttgg gccgggacct tcgccgtgtg gtggacgcgg ccccatgagt tcatccatgg
    10141 tcaattggcc gtcgccattt ttgtccagtt tcatgagaga cgccggcgcg tttgcgattt
    10201 catcggcaga aatgatgccg tcaccattcg tgtccaacgc tgcgacgatg ggcggaatcg
    10261 ggcgatgcgg ccgggcttga ttggaatctc cctgagcgtc aggaggcggg ttgttggtat
    10321 ctccatttct attaggacgc ggcggcagat actcgtcttt cgtgagctgg ccatcgccgt
    10381 ttttgtccaa cgtcaacaac gccgcggagg cgtttgcgat ttcatcggcg gaaatgattc
    10441 cgtcgtgatt agtgtccagt gcctggatga gtggcatcgg cggatgatga cgatggccag
    10501 ggccagggcc gccgccggga gggccgtcat tttggttttg cgcgagcagg acgctggccg
    10561 gcagcgccaa tgccaatgcg aggacgatga ttgattttgc tttcatgcga gtttgtttcg
    10621 tgttcgccgc tttcacgttg ccgcgtcatt gccgcaatcg gaaaccgagc gtttcattag
    10681 taatagactc cggcaatgtt aagcatctat ttgggccggg caggaaaatt gtaaagggaa
    10741 tgtaaaagtg tcggtatttt cccggggatt cgctaagctg aagccattcg caagccattt
    10801 tgcggattga caatgaacaa tcaaacgcaa cccagcaacc ccagcaacgc cgcgccgggc
    10861 cgcacgcggg tgctcgtcat cgatgacgat cggaaactgt gccgtttgat ccgcgattac
    10921 cttgagccga tgggatacga aatcgagatg tctcattccg ggccggaagg actggagaaa
    10981 gcgcagggcc aaaaattcag cgcggtgatt ctcgatttga tgctgccggg aatggatggg
    11041 ttcgaggtgt tgaaaaaatt gcgccacact tccgacgtgc cggtgctgat gctcaccggg
    11101 cgcggcgagg aaccggaccg catcgtaggt ttggaactgg gcgcggacga ttatttgccg
    11161 aaaacttttt cgacgcgcga attgctggcg cgtctgcggg cggtgacacg cagggcggcg
    11221 cgcaaatctt cgggcatgcc ggagactgcc gaggaaattg tcatcggccc gttgcgggtg
    11281 cgcccggaat cgcgcgtggc attattggcg gaccaaccat tggtgcttac gccggttgaa
    11341 ttcgacctgc ttgcctgcct ggcgcgagga aagggccgcg tcaaaacccg cgagcaattg
    11401 ctcgacgcca tcagtgaccg caattacgac gccttcgacc gttcgataga cgtgcatgtc
    11461 tcagcgctcc gaaaaaaatt gggcgatgat ccgaaaaacc cgcgcttcat caaaacgctg
    11521 cgggtggcgg gctacatgct gatcgacccg gaaagtgaag tcgctcaatg aagataagat
    11581 ttccattgta tgcgaaaatc ctgttctggt ttttcctgaa cgtggttttt ctctgcgcgg
    11641 ttttctttat tgttgcgcgc gggcagtttc atttttggat ggatgcgctc atctcaggcc
    11701 cggctggtgg gcggatgcaa gcagtgacgc atttgattgc ggaagaattg cgcgaccgtc
    11761 cgcaaacgga atggaacgga gtgctgcagc gcttcagcag cgcatataat gtcgagttca
    11821 cggtgtttcg caacgacggc cggcagcttg ccggagaaac ggtgacgctg ccgccgcaag
    11881 ttcgcatccg gttggccgag cggcatggaa tgaatccacc gccgccgcat ggtgaaatcg
    11941 aaggcggtca tttcaatccg cctccaggtg atgaaatgca cgctccgcca gccccggaca
    12001 ggccgcaaaa aatcatgatg cacaccagtg cgccaagccg gtattgggta atggttcatt
    12061 tgttgatgcc atcgcaggat gcgtcgccgc atccgacgcc catggttttg ctggcggaat
    12121 cggagagctt gagcggcgga ggattgttct tcgatttgac gaacttggct gcgggcgggt
    12181 tggcggttgt cttggtttcg gtattgttct ggtttccgtt ggtgcgaggc atcacaagtt
    12241 ccatctcacg aatgaccatg gccaccgagc agatagcgga aggacgattt catgtgcgga
    12301 cccaggtgcg ccgtcaggac gaactgggct ccttgagcga agccatcaat cgcatggcgg
    12361 aacgattatc ggggtttgtc gccgggcaga aaagattcct tggggacatc tcgcatgaat
    12421 tgtgctcacc gattgcgcgt attcaagtcg cgctcggcat ccttgaacaa cgcgcggacg
    12481 agaagcagaa ggcttacctg gccgacttgc gcgaagaagt ggaacaaatg tccggcctcg
    12541 tcaacgaatt gctttcattt tccaaggctt cgctgggcgg cgcagaaatc aaacttcaac
    12601 cggtcgcatt gcgc
//
LOCUS       DASZMM010000554         4340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5479590, whole genome shotgun sequence.
ACCESSION   DASZMM010000554 DASZMM010000000
VERSION     DASZMM010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5479590"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VG754_13280"
     CDS             complement(<1..343)
                     /locus_tag="VG754_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20235.1"
                     /translation="MSATSERTGKKLFETGKWSFRNPGAWLSCIGIIVLFVALRWNNY
                     DAPLIRDEGEYFYSAALLKQGIAPYANSFLQKPPMIIYTYAFATTVLPNSYWSARFLA
                     YLFVALTTVLLG"
     gene            complement(362..1678)
                     /locus_tag="VG754_13285"
     CDS             complement(362..1678)
                     /locus_tag="VG754_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWF20236.1"
                     /translation="MKNYVLDTNVLLHDPNSLLYFDENNVLIPIDVIEEIDHFKREST
                     ELGQNARTVSRTLDALRGQGTLNHGVKLKNGGNLRIIFHEFAKNGKSEQFILGDSSVD
                     NRILALALDVKKSNPKQPTILVSKDINLRIKADALGLQAEDYETDRVLIKDLYTGMVE
                     ISISAEKMAAFRANGELDLNGGAQYHPNEYCTLTEETNPKRTALTKVDATGKKVVPII
                     DNREGVWGIKPRNREQHFAFDALLDDRIKLVTLMGKAGTGKTLMAMAAGLKRTVLDRE
                     FRRLVVARPTISMGKELGFLPGSLEEKLAPWMQPIHDALEMLSDLNMGHEHRRSNDLM
                     RSGSIVVEALSYIRGRSIAHQFMVIDEAQNLTPLEVKTIITRVGHGTKIVFTGDPYQI
                     DNPYVDSSSNGFNYIISKFRAESVAAHIELQKGERSELAELAANLL"
     gene            complement(1659..2342)
                     /locus_tag="VG754_13290"
     CDS             complement(1659..2342)
                     /locus_tag="VG754_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWF20237.1"
                     /translation="MLELIQFPWSPFCIVQRRILEFAGARFKIINIPSTDRSRVWRMT
                     RERYYGVPIVKDGPEVIFETDNDSQVIAKYLDFKFGLGLFPAEWEGVQSVIWRCIENE
                     IEEMTFKLNDIYWRENVPAAEHLEFLRFKERKFGRGCLDQWRRDQKSLLAELTRRLLP
                     FDEMLYYGPFLLGPQPHFIDFDLVGMLDNFLYSGHYHLPAVHNRLQKWHRRMAHLQSE
                     PITREKLRT"
     gene            complement(2500..3183)
                     /locus_tag="VG754_13295"
     CDS             complement(2500..3183)
                     /locus_tag="VG754_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HWF20238.1"
                     /translation="MSSFVWWPVANIRLPRAPWKRFGLMLLLAAVLLALSIAGFLVNR
                     FVFRHNFHVVSEGQVYRCGQVGAGGLTKMVQEYGIKSILNLRGGARTGVTAWYSAETN
                     AAGQLGVKYYDYELTATQELTDAQLDKLVTIIGSAPKPLLIHCKSGADRTGLVGALYL
                     YKIEGKPAEAADRELTMLYGHIPYLFWGDTVAMDHSFWHYVNSHGPVSGKPNVRQGQP
                     DPHATSNGN"
     gene            3679..4227
                     /locus_tag="VG754_13300"
     CDS             3679..4227
                     /locus_tag="VG754_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWF20239.1"
                     /translation="MSKLRETFLQELMDIYDAEKQLVKALPKVAKAAEHEELKTAIES
                     HLEETEEHVERVERVFEIIGETAKGKKCQAMMGLLEEGQEIIKEKEGDAALICAAQKI
                     EHYEIASYGCLTTWARILGEDEAADVLEETLDEEKNADDKLTSLAESIINEEESQGEQ
                     EEESEGRGRSRMPSSSSTRSKG"
BASE COUNT         1053 a         1159 c         1188 g          940 t
ORIGIN      
        1 ttcccagcag cactgttgtc agagccacaa acagatatgc gagaaaacgc gccgaccaat
       61 atgaattcgg caacaccgtt gtcgcaaacg cataggtgta aatgatcatc gggggctttt
      121 gcaggaagga attcgcataa ggcgcgatcc cctgcttcag caacgcagcc gaatagaaat
      181 attccccctc gtcccggatc aacggcgcgt cgtagttgtt ccaacgaaga gcgacgaata
      241 aaacaattat gccgatgcaa ctgagccagg cgccgggatt gcggaaggac cacttcccgg
      301 tttcgaaaag tttttttcct gtgcgttcgc ttgtggcaga cacgtccatt gctggacggc
      361 cttataacaa attggctgcc agttcggcca gttcagaacg ctcgcctttt tgcagctcga
      421 tgtgcgcggc caccgattcc gcgcggaact tgctgatgat ataattgaaa ccgttcgagg
      481 aagagtccac gtaaggattg tcaatctggt acggatcgcc ggtgaacaca attttcgtgc
      541 cgtggccgac gcgtgtgatg attgtcttca cttccagcgg ggtaagattt tgcgcctcgt
      601 caatgaccat gaactggtgc gcgatgctgc gtccgcgaat gtagctcaag gcctcgacca
      661 caatgctgcc cgagcgcatc aaatcgttgc tgcggcgatg ctcatggccc atgttcaagt
      721 cgctcaacat ttccagcgcg tcatggatcg gctgcatcca tggcgcgagc ttttcctcga
      781 gagaacccgg cagaaagcca agttccttgc ccatggaaat cgtcggacgc gccaccacca
      841 agcgccggaa ttcgcggtcc aacacggtcc gtttcaaacc cgccgccatc gccatgagcg
      901 ttttgcccgt gcccgccttg cccatcagtg tgaccagttt gatgcggtca tccaacagcg
      961 catcaaaggc gaaatgctgc tcgcgattgc gcggcttgat gccccaaacg ccttcccggt
     1021 tatcaatgat gggaaccact tttttgccgg tggcatccac cttggtcaat gcggtgcgct
     1081 tgggattggt ctcctcggtg agcgtgcaat attcgttggg atggtattgc gcgccgccat
     1141 tcaaatcgag ttcgccgttg gcgcggaacg ccgccatttt ttccgcgctg atcgagatct
     1201 caaccatgcc ggtgtaaagg tccttgataa gcacgcgatc cgtctcgtaa tcctccgcct
     1261 gcaacccgag cgcgtcggcc ttgatgcgaa gattaatgtc cttgctgacg agaatggttg
     1321 gctgcttggg attggatttc ttgacatcga gcgccagcgc aagaatgcga ttgtccacgg
     1381 acgaatcgcc gagaatgaat tgttcgcttt ttccattctt tgcgaattcg tggaaaatga
     1441 tgcgcaggtt tccgccgttc ttgagtttga caccgtgatt caacgtgcct tggccgcgca
     1501 aggcgtcgag cgtgcgcgaa accgtgcggg cattctggcc caactcggtg gattcgcgct
     1561 tgaaatgatc gatctcctcg atgacatcga tggggatcag aacattgttt tcatcgaaat
     1621 aaagcaggct gttggggtcg tggaggagaa cattggtgtc aagtacgtag tttttcacgt
     1681 gtgatgggtt cggattggag atgggccatg cgacgatgcc atttttgcag ccggttgtgg
     1741 acggcgggca gatgataatg gccggagtaa agaaaattat cgagcatccc gacgaggtcg
     1801 aagtcaatga aatgcggttg cggcccaagc aggaacgggc cgtaataaag catttcgtcg
     1861 aagggcaaaa ggcggcgcgt gagttctgcg agcaacgatt tttgatcgcg acgccactgg
     1921 tcaaggcaac cacgtccgaa tttgcgttcc ttgaaacgca aaaattcgag gtgctcggcg
     1981 gcaggcacgt tttcgcgcca gtaaatgtca ttgagcttga atgtcatctc ctcgatttca
     2041 ttctcgatgc agcgccagat gacggattgc acgccctccc attcggccgg aaacaaaccg
     2101 aggccaaatt tgaaatcgag atacttcgcg atcacctgcg aatcgttatc ggtctcaaaa
     2161 atcacctccg gaccgtcctt cacgatggga acgccgtagt aacgctcgcg cgtcatccgc
     2221 cagacacggg aacggtcggt tgaagggatg ttgatgattt tgaagcgcgc gcctgcaaac
     2281 tccaggatac gcctttggac aatgcagaaa gggctccagg gaaactgaat caattcgagc
     2341 atggctattg gtttcgcacg aacgccgaat caataagttt tagacgcatt ttaagcgaca
     2401 cacgacaaaa ttaaatccac gccgccccgc tgacaagggg aaatcccaat gtttcacatc
     2461 tgtaaccatt catttttaga ggctttggaa aggctcgcat cagtttccgt tggatgtggc
     2521 atgagggtct ggttggccct gccgaacgtt cggtttgccg gacaccggcc catgactgtt
     2581 cacataatgc caaaaggagt ggtccatcgc gaccgtatct ccccaaaaaa gataagggat
     2641 gtgcccatac aacattgtca gttcgcggtc ggctgcttcc gcaggtttgc cctcgatttt
     2701 gtacagataa agcgctccaa ccaatccagt gcggtcagcg ccgcttttgc aatggatcaa
     2761 cagcggcttg ggagcgctgc cgatgatcgt cacgagtttg tccaactgag cgtcggtcaa
     2821 ttcctgtgtg gcggtgagtt cgtaatcgta atatttgacc cctaattggc ctgcggcgtt
     2881 tgtttcggcg ctataccaag cggtcacacc ggtccgggcg ccgccacgca ggttcaagat
     2941 gctcttgatg ccgtattcct gcaccatttt ggtcaaacct ccggcaccca cctggccgca
     3001 acgataaacc tgcccttcgg aaaccacatg aaaattgtgc cgaaatacga aacggttcac
     3061 aagaaaaccg gcaatcgaca gcgcgaggag aacggcggca agcagaagca tcaggccaaa
     3121 acgcttccat ggcgcgcgcg gaaggcgaat attcgccacc ggccaccata cgaacgaact
     3181 catgcgtgat gcacccatga cgctagccta tcaatacccg cagcgagcaa cttagtcgat
     3241 aagaaaatgg ttgagcgcat cggcgcactg ccccggagcg cgggtcgatg acccgcagca
     3301 atgtgggaat accggctgtg tccgaattag tcgtgcgtgt cctggttctg atgcgttgct
     3361 gcggctcacc gagccgcgct ccgttttacc cggtgaacgc cccggcaagc tcaacccatc
     3421 cggcatgacc gacaaatccc gacagtgcca agacgggccc gttctcaggg gtgcatcttg
     3481 acactgttct tgagaatgta cgggtacggt ttgcttgcgg ccgcttcgcg aaagtgttca
     3541 aaaaaaattc cacggaccaa cgggttgttt acctactgtt gcatcgctca taaaaatgga
     3601 aacgtgaatg gcactggcga gccatctgaa tggagtttcg tcacgtcaca aacaaaaaca
     3661 atcaacaaga ctgggtttat gagcaaacta cgcgaaacgt ttctacagga attaatggac
     3721 atatacgacg ctgaaaaaca actcgtgaag gcgctgccaa aagtcgccaa ggccgccgag
     3781 cacgaggaat tgaagaccgc gattgaatcg catctggagg aaaccgagga acacgtcgaa
     3841 cgtgtggaac gcgtgttcga aatcatcggc gaaaccgcga agggcaagaa atgccaggcg
     3901 atgatggggc tgctggagga agggcaggaa atcatcaagg agaaagaagg cgacgccgcg
     3961 ctcatctgcg ccgcgcaaaa gatcgagcat tatgaaatcg cttcctacgg ctgcctgacc
     4021 acttgggccc gcatcctggg cgaagacgaa gccgcagacg tactcgaaga aacactggac
     4081 gaggaaaaga atgccgatga caaactgacc tcgcttgccg aaagcatcat caacgaagag
     4141 gaaagccagg gcgaacagga agaagagtct gaaggtcgtg gacgttcccg gatgccctcc
     4201 tcctcgtcca ctcgcagcaa aggttgaaaa gcgcgctcga catcgaatct ccaacgctga
     4261 aagttcagcg ttcggagttc gatggggctg ttgcatttct ttcttccatc aacccacccg
     4321 aaaagagatg cgggtgatca
//
LOCUS       DASZMM010000555         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2722469, whole genome shotgun sequence.
ACCESSION   DASZMM010000555 DASZMM010000000
VERSION     DASZMM010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2722469"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VG754_13305"
     CDS             complement(<1..1057)
                     /locus_tag="VG754_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWF20240.1"
                     /translation="MKLLLLSVLLAAGLARSLMAQTNIVNEASQLEFKGHFKEAAAVL
                     QSGIESGKASPAELKTLKFELDRLGRIRQDYPLMRDDLFEQLKGTVKDLTPEEFNQWI
                     TKGWFDNREIDGRIYYFNSEPNNIFFRHPELNPRRIDPEDAKWLESKRLELIHSIKKA
                     SQDEKNPYVLPMIFNVTMRVTADANAAPDGETIRAWLPIPRTYPFQTGFKLLSSSPTP
                     KSIDAEDSSIRSIYFEESAHAGKETQFKIDYEFTVRGVHFDLQADKIQPFDPNDEAVK
                     KFTGEAPHVVFTPEIKALSAKIVGNETNPMLKAKKIYDWLSDNLQYSYALEYSTIRNL
                     SDYTRSHCYGDCGQQALF"
     gene            complement(1059..>3339)
                     /locus_tag="VG754_13310"
     CDS             complement(1059..>3339)
                     /locus_tag="VG754_13310"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWF20241.1"
                     /translation="VAELPGTFLTIATTRPETIPGDTAVAVNPKDTRYAHLIGKHVIR
                     PLPRELPREQKLIPIIGDEHVDFEFGTGVLKVTPAHDKADFEIGLRHKLAVVDIMNPD
                     ATMNDLAGEDLKGLDRFKARKVAVEKLVELGALEKEEPYTNKVGFSERADVPIEPRLS
                     EQWFLKYPSVEKARACVATGEMKFHPERWAKVYDHWMGNIQDWCISRQLWWGHRIPVW
                     HGWASNFDLAFFRAACSDGFKKGKIECSLEGSTEADATLREAEFIRAEFVESEQKWYV
                     WHCVQPGFEHFEKIIEDRGYKRDPDVLDTWFSSWLWPFATMGWPEQTPTLKKFYPTTD
                     LVTGPDIIFFWVARMIMAGYEFMGEMPFRNVYFTGIIRDKQGRKMSKSLGNSPDPLDL
                     IEKYGADALRFGVMRSAPLGQDILFDEQHVELGRNFCNKLWNACRFRQMQGSAGVPPA
                     YEGVSPEQSQTFEVQGEIVPQLLTGDDKWILLKLNTALRELSTSLAEYKFSEATQTLY
                     RFFWGEFCDWYLEATKAVFQSEDEARKANSLAVIDFVLSHTLRFFHPFLPFITEELWH
                     GMGYSADMPENQGGQSIMYAPWPKPFDEDFLGHYGLDDCYLEMADKKYELVTQGRNLR
                     REANIQSGKKVKFILKPSGHISEHDVAVLKILLNAEALEVDPNYIPKKGTPVVHSELG
                     ELYLPLEGLIDIAAEKARLTKELDKINAEIVKVEQKLANPAFTQKVPIEVLKEHEKRL
                     ADWQAKKQHVLAALAALEG"
BASE COUNT          722 a          927 c          873 g          817 t
ORIGIN      
        1 agaatagcgc ttgttggccg caatcgccgt agcaatgcga acgggtgtaa tcactgaggt
       61 tgcggatggt ggaatattcg agcgcgtaac tgtattgcag gttgtcggaa agccagtcgt
      121 agattttctt cgctttcagc atcgggtttg tttcattgcc gacaattttg gccgagagcg
      181 ccttgatttc aggcgtgaac accacatgcg gcgcttcacc ggtaaatttt tttaccgcct
      241 cgtcattcgg atcgaaaggc tggattttgt ccgcctgcaa atcgaaatgc acgccgcgta
      301 ccgtgaattc gtaatcaatt ttaaattgtg tttccttccc cgcgtgcgcc gattcttcga
      361 agtaaatgga gcgaatggaa ctgtcctcgg catcaatgga tttgggcgtt ggcgaactgg
      421 agagcagttt gaaaccggtt tgaaacgggt aagtccgcgg aatgggaagc catgcgcgaa
      481 tggtttcacc gtcgggcgcg gcattcgcat cggccgtgac gcgcatggtg acgttgaaga
      541 tcattggcag gacgtaaggg tttttttcgt cctgggaagc ttttttaatg gaatggatga
      601 gttccagcct tttgctttca agccactttg catcctcggg atcaatgcgc cgtggattca
      661 gttcgggatg gcgaaagaaa atgttgttcg gctctgaatt aaaatagtaa atcctcccat
      721 caatttcgcg attatcaaac caacctttgg taatccattg attgaattct tcgggggtca
      781 agtccttcac cgtccccttg agttgctcaa acaaatcatc gcgcatgagg ggatagtcct
      841 gccggatgcg tccgagccga tccaattcga acttgagggt cttcaattcc gcaggagaag
      901 ccttgcccga ctcgatgccg ctttgaagga cggcggcggc ttccttgaaa tgccctttga
      961 attccagttg cgatgcctcg ttcacgatgt ttgtttgcgc catcaggctg cgcgccaggc
     1021 cggcggcgag caaaactgaa agcaaaagca gcttcatatc agccttccaa tgcggccagc
     1081 gccgcgagga catgctgctt tttcgcctgc cagtcggcaa gccgtttctc gtgttccttc
     1141 aagacttcaa tcggcacttt ttgcgtgaat gccgggttcg caagcttctg ttcgaccttc
     1201 acgatctccg cgttgatctt gtctaattcc ttcgtcaacc gcgctttctc ggctgcgatg
     1261 tcaatcaaac cttccaacgg cagatacaat tcgcccaact cggaatgcac gaccggcgtc
     1321 ccctttttcg gaatgtaatt gggatcaact tccagcgcct cggcattcag caaaatcttc
     1381 aaaaccgcaa cgtcgtgttc tgaaatatgt ccggacggtt tcaagatgaa cttcaccttc
     1441 ttgccggact ggatgttcgc ttcgcgtcgg agattgcggc cttgcgtgac cagttcgtat
     1501 tttttgtcgg ccatttcgag gtagcaatcg tccagaccat aatgaccgag aaaatcctcg
     1561 tcgaacggct tcggccacgg cgcatacatg atgctttgcc cgccctggtt ttccggcatg
     1621 tccgccgagt aacccatccc gtgccacaat tcctccgtga tgaacggcag gaacggatga
     1681 aaaaaccgca gggtgtgcga cagcacaaaa tcaatcaccg caagcgagtt cgccttgcgc
     1741 gcttcgtctt cggactggaa caccgccttg gtcgcctcga ggtaccaatc gcaaaattcg
     1801 ccccagaaaa accggtaaag cgtttgcgtc gcctcgctga atttgtattc ggccagcgaa
     1861 gtgcttagtt cacggagcgc ggtattcagc ttgagcaaaa tccatttgtc gtcgccggtg
     1921 aggagttgcg ggacgatttc gccctggacc tcgaaggttt gggactgctc gggcgagacg
     1981 ccctcgtatg caggcgggac gcctgcgctc ccctgcatct ggcggaaacg gcaggcattc
     2041 caaagcttgt tgcagaaatt gcggccgagt tcgacatgct gttcatcgaa caaaatgtcc
     2101 tgaccgagcg gcgccgagcg catcactccg aaacgcagcg cgtccgcgcc atatttttcg
     2161 atgaggtcga gcggatccgg cgaattgccc agcgatttcg acatcttgcg gccctgcttg
     2221 tcccggatga tgccggtgaa ataaacattg cggaacggca tctcccccat gaactcgtaa
     2281 cccgccatga tcatgcgggc gacccagaag aaaatgatgt ccggtcctgt gacaagatcg
     2341 gtcgtgggat aaaacttctt gagcgtcggc gtctgctccg gccagcccat cgtggcaaac
     2401 ggccagagcc aggaactgaa ccaggtgtcg agaacgtcgg ggtcgcgctt ataaccacga
     2461 tcctcaataa ttttttcgaa gtgttcaaac cccggttgaa cacaatgcca gacgtaccac
     2521 ttctgctcag attcaacgaa ttcagcccgg ataaattcag cctctcgcaa ggtcgcatcc
     2581 gcttcagtcg aaccctctaa agagcattcg attttgcctt tcttgaagcc gtccgagcag
     2641 gcagctcgaa aaaaggcgag gtcgaagttt gatgcccatc catgccacac cggaatccga
     2701 tgcccccacc aaagctggcg gctgatgcac caatcctgga tgttgcccat ccaatggtcg
     2761 taaacctttg cccaccgctc gggatgaaat ttcatttcgc ccgttgccac gcacgcacgc
     2821 gctttttcca cgctcggata tttcaaaaac cattgctcgc tcaagcgcgg ctcgatgggc
     2881 acatcggcgc gttcgctgaa accgactttg ttcgtgtaag gctcttcttt ttcgagcgcg
     2941 ccaagctcca cgagtttttc cacagcaact tttcttgcct tgaatcgatc caagcctttc
     3001 aaatcctcgc cggccagatc attcatcgtc gcgtccggat tcatgatgtc cacgaccgcg
     3061 agcttgtgcc gcaaaccaat ttcaaagtcc gccttgtcat gcgccggcgt cactttcaac
     3121 acgcccgtgc caaattcaaa atccacatgc tcgtcgccga tgatgggaat gagcttttgt
     3181 tcgcgcggca attcgcgggg caggggacgg atgacgtgct tgccaatgag atgcgcataa
     3241 cgcgtgtcct tcgggtttac tgcgacggcg gtgtcgccgg gaattgtttc cggacgcgtc
     3301 gtagcgatgg tgagaaacgt tcccggcaat tcggcgact
//
LOCUS       DASZMM010000556         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4920412, whole genome shotgun sequence.
ACCESSION   DASZMM010000556 DASZMM010000000
VERSION     DASZMM010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4920412"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VG754_13315"
     CDS             complement(<1..462)
                     /locus_tag="VG754_13315"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HWF20242.1"
                     /translation="MNFLSPFAFLFALTIPVVIVFYLLKRKRVVKLVSSTLLWQKFLA
                     ETQASAPFQKLRRNWLLILQIILLILAVLALTRPYFATKAKSSDLRVLILDASASMKS
                     TDVSPSRFEKAREEALKWVDSLRPSDKMVVLQVAANTEVKQSETSEKAALRR"
     gene            complement(875..1774)
                     /locus_tag="VG754_13320"
     CDS             complement(875..1774)
                     /locus_tag="VG754_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWF20243.1"
                     /translation="MPNAPLLTPELLRRLEQFQLLAARRAKSSARGERRSRARGQSVE
                     FADHRNYVPGDDFRYLDWNLFGRLEKLFLKLYEEERELPVRIFLDASESMSFGEPRKF
                     DFARQIAAAVGYVALCGFDRVSVIPFPDNKEEMAVRGALRSVRGRKSTMNFFQNITLL
                     TAKGTANFNEALRRGALEARQAGVAVVLSDFLDPAGYESGLTALVGRGFQVNAVQILA
                     PEELSPSTFGDLRLVDAETGSVQEVTFGRYRMNSYQQMVQNFCQKLREYCQSRGINFF
                     MASSNTALEYLLLKQLRQSEVWG"
     gene            complement(2190..3191)
                     /locus_tag="VG754_13325"
     CDS             complement(2190..3191)
                     /locus_tag="VG754_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWF20244.1"
                     /translation="MNTEEQIASFKQSYAALRGEIGKVIVGHDAIVEGTLIAVLAGGH
                     VLLEGVPGLGKTLLVRTLSEVLDLSFNRIQFTPDLMPADILGTNMVMETPDGRREFEF
                     QRGPIFAHLILADEINRATPKTQSAMLEAMQEKSVTAGGEIRKLIEPFFVLATQNPID
                     QEGTYPLPEAQLDRFFFKLVVGYPSAEELTEVLTRTTENARATVNKVLDREALLELQR
                     LVRQVPVATHVKDYAVRLVLATHPKTETAVAITNQYLRFGSSPRGGQCLLLAGKVRAL
                     TQGRFNVSFDDIQAVATAAMRHRLILNFEAEAEGITTDQIIAQILQDVPRDAAMVNA"
     gene            complement(3194..3613)
                     /locus_tag="VG754_13330"
     CDS             complement(3194..3613)
                     /locus_tag="VG754_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWF20245.1"
                     /translation="MPTDKELTGRIIGAAINVHKELGPGFIESVYESALAVEFDLLGI
                     KYERQKTIDVFYRNKKVGEHRLDFLVEGAVVVELKAVVNLEKIFFVVTRSYLKATGKQ
                     TGLLLNFASMPLTIKRVSPEDLNYFSNHSSVHEFLLE"
BASE COUNT          884 a         1060 c          996 g          878 t
ORIGIN      
        1 gcggcgcaac gcggcctttt cgctggtctc ggattgcttg acctcggtgt tcgcggcgac
       61 ttgcagcacg accatcttgt ccgaaggacg caggctgtcc acccacttca acgcttcctc
      121 gcgcgccttt tcaaaacgcg acggcgatac atccgtgctc ttcatcgaag ccgaggcatc
      181 aaggatcaaa acgcgcaaat cgctgctttt ggctttcgtg gcgaaataag ggcgcgtcaa
      241 cgccagcacg gcaaggatga ggaggatgat ttgcaggatg agcagccagt tgcggcgaag
      301 tttttggaac ggcgcgctgg cctgggtttc tgcgagaaat ttctgccaca acaacgtgct
      361 cgaaaccagt ttcaccaccc gcttgcgctt cagcaaataa aacacgatca ccaccgggat
      421 ggtgagcgca aacaggaacg caaatggcga caaaaaattc atctttgcaa gtgtggagtg
      481 cttttgatgt gcaaattcac agactttaac ttcatggaat cccgaaggga ttctggccca
      541 aagcccaggg ttgcgccgca ggcgctaccc tgggacacgc gcgaaaaacg aatccaaccc
      601 caaaggggtt gtggcgcgcc gattccgttc gatgacacaa ccctttcaag gttgttgaaa
      661 tatcgagacc ctttacccag ggtagctcgt tcctcgcaac cctgggctga gtgacacaat
      721 cccttcggga ttgagcgatt gcgctttaca gcagcaatga catccaagcc ggaccttttc
      781 gtccgctgct ttagattttc attactcatc gtttcaaaat tccgcattca atttttgccc
      841 attgcgccaa aggccattct tcattccgca ctcactagcc ccaaacctcg gattggcgga
      901 gttgtttcag aagaaggtac tccaatgccg tatttgaact cgccatgaag aaattgattc
      961 cccggctctg gcaatattcg cgcaatttct ggcagaagtt ctgcaccatc tgctggtagg
     1021 aattcatgcg ataccggcca aacgtcactt cctgcacgct gccagtctcg gcgtccacaa
     1081 ggcgcaagtc gccgaaggtc gagggtgaca attcctccgg cgcaagaatc tgcaccgcgt
     1141 tcacctgaaa tccgcgcccg accaacgccg tcaaaccgct ttcataaccg gcgggatcaa
     1201 ggaagtcgct caacaccacc gcgacacctg cctgccgcgc ttccaacgcg ccgcgtcgca
     1261 aggcttcgtt gaagttggct gttcccttcg ccgtcaatag cgtgatgttc tggaaaaaat
     1321 tcatggtcga tttccggccg cgcacggaac gcaacgcgcc gcgcaccgcc atttcttctt
     1381 tgttgtccgg aaacggaatc acgctgacac ggtcgaagcc gcatagcgcc acgtaaccga
     1441 ccgccgccgc gatttgccgc gcgaaatcaa atttgcgcgg ctcgccaaag ctcatggact
     1501 cgctggcatc gagaaaaatg cggaccggca gttcgcgttc ttcttcgtaa agcttgagga
     1561 acaatttttc gagccggcca aaaagattcc agtcgagata acgaaaatca tcacccggga
     1621 cgtaattgcg gtggtcggcg aattcgaccg attgaccgcg agcgcggctg cggcgctcgc
     1681 cccgggcgga acttttagcg cggcgcgcag ccagcaattg gaattgctcc aagcggcgca
     1741 aaagttcggg agtgaggagg ggcgcgttag gcatgtctta cgaaaaaaaa gggtttaaaa
     1801 gggcaacaaa ctgccgcatg caagacgtaa gagacgcctc caagaaattc gccgaaggcg
     1861 aacagcgtga atagccgtgg gtgaaaaccc acggtaagat gataagatca tcacggccct
     1921 gaaagggccg gactgatcgc atcaatttaa tccaaatatt tttcatcaaa tgccaccccg
     1981 aatttttcca gcattccccg cagttcatca cggaacgaca atcgtttatg atgctgctcc
     2041 tgatgtttga tatactcaat cactccatcc ctgtctgaat gagaaattgt gaaggcagcg
     2101 tattcacgtt tttccgcttc gcggaaacag ggcgcgagaa gattggttgt tcggtgtctg
     2161 gcattttatt tttcttcaga agaacccaat cacgcattca ccatcgcggc atcccttgga
     2221 acgtcctgca aaatttgcgc gatgatctgg tcggtggtga tgccttcggc ttccgcttcg
     2281 aaattcaaga tcaaccgatg ccgcatcgca gccgtggcga cggcctggat gtcgtcgaaa
     2341 ctcacgttga accgtccctg agtcaacgcg cgcaccttgc cggcgagcag cagacattga
     2401 ccgccgcgcg ggctgctgcc gaaacgcaga tattgattag tgattgccac ggcggtttcg
     2461 gttttcggat gcgtcgcgag cacgaggcgc accgcgtaat ctttcacgtg cgtggcgacg
     2521 ggcacctgcc gcacaagccg ctgcaattcc agcaacgctt cgcggtcgag caccttgttg
     2581 accgtggcac gcgcgttttc ggtcgtgcgg gtgagcactt ctgtcaactc ttccgcgctc
     2641 ggataaccaa ccaccagctt gaaaaagaaa cggtccaact gcgcttcagg cagcggataa
     2701 gtgccctcct ggtcaatcgg gttttgcgtg gcaagaacga aaaacggctc gatcaatttg
     2761 cggatctcgc cgccggcggt gacgcttttt tcctgcatcg cttcgagcat cgcggactgt
     2821 gtcttgggcg tggcgcgatt gatttcatcg gcgagaatca gatgggcaaa gattggcccg
     2881 cgctgaaatt cgaattcgcg gcgtccatcg ggcgtttcca tgaccatgtt ggtgccgagg
     2941 atgtccgcgg gcatcaagtc gggcgtgaac tggatgcggt tgaaggaaag atcgaggact
     3001 tcgctgagcg tgcggacaag cagcgtcttg ccaaggcccg gaacgccttc gagaagcacg
     3061 tgcccgcccg cgagcacggc gatcaacgtg ccttccacga tggcgtcatg gccgacgatg
     3121 accttgccga tctcgccgcg caacgccgcg taagattgct tgaaagatgc gatttgttct
     3181 tcggtattca tatttattca agaaggaact catgaactga ggaatgattg gaaaaataat
     3241 ttagatcttc gggagaaaca cgtttaatgg tgagcggcat ggaggcaaaa ttcaagagca
     3301 atccagtctg cttgccggtt gctttcaaat aagaacgtgt aacaacaaag aaaatttttt
     3361 ccaggttcac aacagctttt aattccacca ccaccgcacc ctcaaccaga aaatcgaggc
     3421 gatgttcgcc tactttttta ttccgataga aaacatcgat cgttttctgt cgttcgtact
     3481 ttattccaag caggtcaaat tccacagcca atgccgattc ataaaccgat tcgataaatc
     3541 cgggtcccaa ttccttgtga acattgatgg ctgcgccgat gattcgtcca gtcagttctt
     3601 tatctgttgg cattgttcct gagtttctga gttccttctt gaacaagcct actttttcat
     3661 gtcgtcgaaa taatcgcgca cggcgccctg atacgcgcgc ggcacttttt cctggctcaa
     3721 cgcgctttgg gcatcggctt gcgcggcgac ggcggcttct tcataagcaa ccttgctctg
     3781 gcctttgatg ctgatgcctt ttaacgtgat gctcggca
//
LOCUS       DASZMM010000557         5985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3545529, whole genome shotgun sequence.
ACCESSION   DASZMM010000557 DASZMM010000000
VERSION     DASZMM010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3545529"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VG754_13335"
     CDS             complement(<1..926)
                     /locus_tag="VG754_13335"
                     /inference="COORDINATES: protein motif:HMM:NF027711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5005 domain-containing protein"
                     /protein_id="HWF20246.1"
                     /translation="MKARRHKILCNRPTRHRWALRCRWSLLAGVVLLVANLGQAQILT
                     NGGFESGLAGWSSSFSSGGVGTFANVADYIHSGSSALEVTVSNPGTATNSIRIVSSSF
                     NANSSNTYVLRFWANTDTLDANMGVNLLGTTPAYPQIPFQLSTNSLAAGDEHYQEYLY
                     AFRASGTVLIAFNFQTVAKYWLDDVQVLDMTDNDGFDVSMTYLWQWGQFNFIRTNSLK
                     IGWTGGDNDKSRLLPDGSVAWIFNDSYATTLSTNSFYSNIRGNSSLPRNCVVHQVGTN
                     LVWMNKGANTFFVPTNSADLYWIGDSVVESNK"
     gene            1365..2216
                     /locus_tag="VG754_13340"
     CDS             1365..2216
                     /locus_tag="VG754_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF20247.1"
                     /translation="MKQQKTERRAGGFTLIELLVVIAIIAILAALLLPALSKAKLRAT
                     GVSCLNNLKQLTLAAHIYASDNQDYIVPNIIGVNNSWVGGNVNGSSLDPTGPTNTAPI
                     MASLLFQYDKSVENYRCPADLVGIAAMAGALRARSYSLNCMMGDNNDPGGTGDNVHAG
                     IKENIKFTSVVNPGPSDASFFIDEQAGSTPTTTSIDDGYFAVSYNAIGPTWQNVPASR
                     HGNHGQISFADGHAGVMKWRGAKTQYLQGWNGAANSGVRPDLDLQQLWLSTYAIGGYP
                     GKPSPWN"
     gene            2352..5900
                     /locus_tag="VG754_13345"
     CDS             2352..5900
                     /locus_tag="VG754_13345"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HWF20248.1"
                     /translation="MNFKISRLFLHFALAYFTLTASAADPLATGFLNPPDSAKPQTWW
                     HWMNGNITKEGITADLESMKQIGLGGATIVNVDCDIPPGPVKFMSPEWRDDFKFAVEE
                     AHRLGLKLCVENCAGWSSSGGPWNTVTNAMQRLTSSELAVTGPTNFDASLPLPPVNLG
                     FYHDIGVFAFKDPSAKADTAAAADSTPAKLEIKHAVYEGENNGGSADVTARLAGLIRS
                     GHKSVIVNDAQMGGDPAPFSLKQLRVELTLNGTPAAFTVGQGDTLVFPVDTNQLAALS
                     SFDNTLAGRTFVRPPAASIPFVGAAIPHDGVVDLAAKVGADGRLRWKIPPGNWIILRL
                     GYTPIGVNNHPAPKEGEGLECDKLNKAALNAHWDGFMQKVLADIGPLAGETLNASLID
                     SYEIGNQDWTGDFRNEFEKRRGYDPLPFLPAFNRYVVDDPAVTERFLWDMRRTVADLF
                     AENYYAHFNELCKQHGLLSAIEPYTGPFESLPCGGPADVVMGEFWAGSQGDPSVKMAA
                     SIAHIYGKTIVGAESFTGWPNDGRWQDDPYSLKPLGDLMFSEGINRYIFHRYAMQPWT
                     NRWPGMTMGPFGINFERTVTWWNQGDAWIKYISRCEYLLQQGRTVADAAYFDGQSAPV
                     VIRAGNPPLPTGYDYDCVDADVLLHATVEKNGRIMLPSGANYAVLILPPNDINMTPPL
                     LACLRKLVRSGATIVAPRPQHSPSLENYPECDRQVKQMADELWGSCDGKTVLEHGFGE
                     GHAVWGKSLADLFASQNLKPDFEFQGASTGVRLVYTHRLDGPADIYFVSNQRRQADSV
                     ECTFRVNGRIPELWHPDTGVMETAPIWNIHEGRTTVRLDFDAAGSVFVIFRRPAGRAD
                     HVISAETIAGDSIASQLQITHAIYAGGMDVTTKLSGLVRNGELAVKVGNETFGSDPAK
                     MHPKELRVDYVIDGMPDQTIVPEGEVLTLSGKANVGATPQWEASLAADGSPVVKAWSN
                     CRVQLKTAEGKVLHAEATSVPAAREITGPWNLTFPPNWGAPPSVTLDKLISWTDHTNN
                     GVRYFSGTATYKKEIEIPAQQISAGGQLWLDLGTVKNFAEVFLNGQDLGVLWKPPFRV
                     NITAAVRTGANKLVVKVTNLWPNRIIGDEQLPPDCEWNSDKLRAWPQWLLEGKASPTG
                     RFTFETWHHYSKKSPLLKSGLLGPVTLHTVKIIPAN"
BASE COUNT         1364 a         1644 c         1652 g         1325 t
ORIGIN      
        1 aatttattgc tctccaccac gctgtcgcca atccaataca aatccgccga attggtcggc
       61 acaaaaaagg tgttggcgcc cttgttcatc caaacgagat tggtgcccac ctgatgcacc
      121 acgcaattgc gcggcagcga actgttgccg cggatgttgg aataaaaact attggtgctc
      181 agcgtcgtgg cgtaagagtc gttgaagatc caggccacgc tgccatccgg cagcaggcgt
      241 gacttgtcat tgtccccgcc cgtccacccg attttgagac tgttggttct gataaaatta
      301 aactgtcccc actgccacag atacgtcatg gaaacgtcaa agccatcgtt gtccgtcata
      361 tccagcacct gcacgtcatc cagccagtat ttcgccacgg tctgaaagtt aaaggcaatc
      421 aagaccgtgc ccgatgccct gaaggcatag agatattcct ggtagtgctc atcacccgcg
      481 gcgagcgaat tggtggaaag ctgaaagggg atctgcggat aggccggcgt cgtccccagc
      541 aagttaaccc ccatgttggc atccaacgta tccgtgttcg cccaaaagcg caggacgtag
      601 gtattgctgc tgttggcgtt aaacgaactg ctgacgattc gaatcgaatt ggtggcggtg
      661 ccaggattgc tcaccgtcac ctccaaagca ctgcttccac tgtgaatgta gtcagcgaca
      721 ttggcaaaag tccccacgcc tccactggaa aaactgctac tccaaccggc caggccggac
      781 tcaaacccgc cattcgtcaa tatttgcgcc tgccccaaat tcgccaccaa cagaacaacg
      841 cctgcgagca acgaccagcg gcatctcaat gcccagcgat gccgggttgg acggttgcat
      901 aatattttat ggcgtcgcgc cttcattaaa ttcaaacgga caatcggtcg cttttttatt
      961 atatccgtta agccaagacg tggcttggaa tagttgagtg aagagccttt atagccaaaa
     1021 taaaccagat tgccgcagga gtatcgtgcc caaaaccggg ggataaagtg ccaaaagcgc
     1081 ctctctttac tgaaacgtgt gcaaaagtcg tcgaaaaaac acagcggaag ggttatccgg
     1141 cacgctggca cttttccacc ccatttttgg cacgtgactc ccgcgttcca ttccgggcaa
     1201 atcgataata ttcaattacc acctgaataa aaatacgcct tgcaacgatt agccgtcatg
     1261 cgacccgaat gaacgaaaga ttcaaactca tgaactccgt gaagattttc cggaatcagg
     1321 gacccgccgc ggattcggcc gaagattttt cttaaattgg atctatgaag cagcaaaaaa
     1381 cagaacggcg ggccggcggt tttacgctca ttgaactgct ggtcgtcatt gccatcattg
     1441 ccattttggc ggccctgctc ctgcctgcat tgtccaaggc caagttgagg gcgacgggtg
     1501 tcagctgcct caacaatttg aagcagctca cgctggcggc gcatatttat gccagcgaca
     1561 atcaggatta catcgtcccg aacataattg gtgtaaacaa tagctgggtg gggggaaacg
     1621 tgaacggcag ttccctcgat cccaccggtc ccaccaacac cgcgccgatc atggcgtcat
     1681 tactttttca gtacgacaaa agcgtggaaa attaccgttg tccggccgac ctggtgggaa
     1741 tcgccgccat ggccggcgcg cttcgggcgc gcagttactc gttaaactgc atgatgggcg
     1801 acaataacga tccgggaggt acgggcgata atgttcatgc cggcatcaaa gaaaacatca
     1861 aatttacttc cgtggtaaac cccggccctt cggacgcctc tttttttatt gatgaacagg
     1921 ccggcagcac gccgacgacc accagcatag atgatggata cttcgcggta agctataatg
     1981 ccattggtcc cacctggcaa aatgtgcccg cgagccgcca cggcaaccat ggtcagatct
     2041 cgttcgccga tggccacgcc ggcgtcatga agtggcgggg agcgaagacc caatatttgc
     2101 agggctggaa cggggccgcc aattcgggcg tgaggcctga tttggatctt caacagttat
     2161 ggttgtcgac ctacgccatt ggaggctatc ccggcaaacc gagtccgtgg aattaatact
     2221 cctctctgtc cgaagggtta aatcgaagcg atggggcggc gcgatggttt tgtcgttagt
     2281 atcatgttct tccttattta gttttcagaa agttccgctg ttataatctt ttttgtttag
     2341 gcaaaactaa aatgaatttt aaaatatccc gtctattcct tcatttcgct ctcgcgtatt
     2401 ttaccctgac agcatcggca gcggatcccc tggcgacggg ctttcttaat cctccggatt
     2461 ccgcgaaacc gcagacgtgg tggcattgga tgaatggcaa catcaccaaa gaggggatca
     2521 ccgccgattt ggaatccatg aaacaaatcg gcctgggcgg cgcgaccatt gtgaatgtgg
     2581 actgcgacat cccacccggt ccggtgaagt tcatgagccc ggaatggcgc gacgatttca
     2641 aatttgccgt ggaggaggcg catcgcctgg gcctcaagct ctgcgtggaa aattgcgcag
     2701 gctggtccag cagcggtggg ccgtggaaca cggtgacgaa tgcgatgcaa cgcctgactt
     2761 ccagtgaact ggcagttacc ggtcccacaa attttgatgc gtctttgccg ctgccgccgg
     2821 tcaacctcgg tttttaccac gatattggcg tgttcgcttt caaagatcct tcggccaaag
     2881 cggacaccgc cgcggctgcc gactcaactc ctgcgaagct ggaaatcaaa catgccgttt
     2941 acgaagggga aaataatggt ggctcggccg atgtgaccgc ccggctcgcg ggtttgatca
     3001 ggagcggcca taaatccgtg attgtcaacg acgctcaaat gggcggagac ccggcgcctt
     3061 tttcgctcaa acaattacgc gttgagctta cgctcaatgg gactcccgcc gctttcacgg
     3121 tggggcaggg ggacacgctc gtttttccag tcgacacaaa ccaactggcg gcgctttcgt
     3181 cgttcgacaa caccctggcg ggtcgcacgt ttgtccggcc gccggctgcg agcattccat
     3241 ttgtcggcgc ggcgattcct cacgacggcg ttgtggattt ggccgcgaag gtgggcgcgg
     3301 acggccggtt gcggtggaaa attccgccgg gcaattggat cattctgcgt ctcggctaca
     3361 cgccgattgg cgtgaataat catcctgctc ccaaagaagg cgagggcttg gaatgtgaca
     3421 agctcaataa ggcggccctg aatgcgcact gggatggttt catgcagaaa gttcttgctg
     3481 acatcggccc gctcgcgggg gagacgctca atgcgtcgct gattgacagc tatgaaattg
     3541 gcaaccagga ctggaccggc gattttcgga acgagtttga aaaacggcgt ggttatgatc
     3601 cgctgccatt tctgcccgcg ttcaaccgct acgtggtgga tgatccggcg gtcaccgaac
     3661 ggttcctttg ggacatgcgc cgaacggttg ccgatttgtt tgcggaaaat tactacgcgc
     3721 attttaacga gctgtgcaag caacatgggc tgttgagcgc gattgaaccc tacaccggac
     3781 cgtttgaatc gttgccttgc ggcgggcccg ccgacgtggt gatgggcgaa ttctgggccg
     3841 gcagccaggg ggatccctcc gtgaagatgg ccgcgtccat cgcgcatatt tacgggaaaa
     3901 ccatcgtcgg cgcggagtcg ttcacgggct ggcccaatga cgggcggtgg caggacgatc
     3961 cgtattctct caagccgctg ggagacttga tgttttccga aggcatcaat cgctacatct
     4021 tccaccggta tgccatgcag ccgtggacga atcgctggcc gggcatgacg atgggaccgt
     4081 ttggcattaa tttcgaacgg acggtaacct ggtggaatca aggcgacgcg tggataaaat
     4141 atatttcccg ttgcgaatat cttttgcagc aaggccgcac cgtggccgac gccgcttatt
     4201 ttgacggcca gagtgcgccg gtggtcattc gtgccggcaa tcctccgctg ccaaccggct
     4261 acgattatga ttgcgtggat gctgatgttt tgctgcatgc gaccgtggaa aaaaatggcc
     4321 ggatcatgct gcccagtggc gccaattacg ccgtgctgat cttgccgcca aacgacatca
     4381 acatgacgcc gcccctgctg gcttgccttc gcaaattagt tcgttccggc gcgacaattg
     4441 tggctccgcg tccgcaacat tcacccagcc tggaaaatta tcccgaatgc gaccggcagg
     4501 tgaaacaaat ggcggatgag ctatggggct cttgtgacgg caagacggtt ttggaacatg
     4561 gcttcggcga aggccacgcc gtatggggca aatctctcgc cgacctcttc gcctcccaaa
     4621 atctgaagcc tgattttgaa tttcaaggcg cgtccaccgg cgtgcggctg gtttatacgc
     4681 accgcctgga tggtccggcg gacatttatt ttgtttccaa ccaacgtcgg caggccgatt
     4741 cggtcgaatg cacattccgc gtcaacggtc ggattcccga actctggcat cccgacaccg
     4801 gcgtgatgga aactgctccg atttggaata ttcatgaggg acgcaccacg gtgcgtttgg
     4861 atttcgacgc cgccggatcg gtgttcgtaa tttttcgccg gccggccggc cgtgcggatc
     4921 atgttatttc cgcggagacc attgccggag attccatcgc gtcgcagttg caaatcactc
     4981 acgcgattta cgcgggcggg atggatgtga cgacgaaact ctccggcctg gtgcgaaacg
     5041 gcgaattggc ggtcaaagtc ggcaatgaaa cttttggcag cgatccggcg aaaatgcacc
     5101 ccaaagaatt gcgcgtggat tatgtaatcg atggcatgcc ggaccagacg atagtgcccg
     5161 aaggcgaagt gctcacgctc tcagggaaag cgaatgtcgg cgccaccccg caatgggaag
     5221 cgagccttgc cgccgatggt tcacccgttg taaaagcctg gagcaattgt cgggtgcaat
     5281 tgaaaaccgc tgaaggaaag gttttgcatg ctgaggcaac cagcgtgccg gcggcgcggg
     5341 aaattaccgg cccatggaat ttaacttttc cgcccaactg gggcgctccg ccgtccgtta
     5401 ccctggacaa attaatttcc tggaccgacc acaccaacaa tggcgtgcgc tatttttccg
     5461 gcacggcgac ctataaaaag gaaatcgaaa tcccggccca gcagatttcc gccggcggcc
     5521 aactctggct ggacctgggt acggtaaaaa acttcgcgga agttttctta aatggtcagg
     5581 acctaggtgt gttatggaaa ccgcccttcc gcgtgaacat caccgccgcc gtgaggacgg
     5641 gcgctaataa actggttgtc aaggtaacga atctctggcc aaaccggatt atcggtgacg
     5701 aacagttgcc gcccgattgc gaatggaaca gcgacaaact tcgcgcgtgg ccgcaatggc
     5761 ttctggaagg caaggccagt cccaccgggc gattcacctt cgaaacctgg catcattatt
     5821 ccaaaaaatc gccgttgctg aaatccggac tgctcgggcc ggtcacgttg catacagtga
     5881 agatcattcc ggccaactaa accgcctata tcaaatgact gcttctctcg aaaggtaggg
     5941 cgcgtcactc cgtgcgcgcc gttacaattg caacagaagc ggcgc
//
LOCUS       DASZMM010000558         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_816289, whole genome shotgun sequence.
ACCESSION   DASZMM010000558 DASZMM010000000
VERSION     DASZMM010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816289"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VG754_13350"
     CDS             complement(<1..1230)
                     /locus_tag="VG754_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWF20249.1"
                     /translation="MTNLSKARNTTATPETPAPAPTLKKLLVANRSEIAIRVFRAATE
                     LGLRTVAIYAQEDRLSVHRFKADEAYLVGEGKGPVGAYLDIPGIVALARERGVDYIHP
                     GYGFLSENGDFAQACADAGITFVGPRPELLRMMGDKVAARALAEKVGVPTLPGTPNPI
                     EDRAEALRAAKEIGFPLIIKAAFGGGGRGMRVVNKAADLADLLDEARNEAGRAFGNPA
                     VFLEKYIPRAKHIEVQVIGDKQGNVLHLHERDCSVQRRHQKVVEIAPSVGLEPSVRAE
                     LCAAAVRIAREINYDNAGTVEFLLDLDTNAWFFIEMNPRIQVEHTVTETITGVDLVRS
                     QILVAQGHSLHGVDLDLPLQSEIPRNGFAVQCRITTEDPANKFMPDYGRILTYRSAGG
                     FGLRLDGGMGTAGSVITP"
     gene            1353..1820
                     /locus_tag="VG754_13355"
     CDS             1353..1820
                     /locus_tag="VG754_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF20250.1"
                     /translation="MNDADQFTVLLVEDDLNDIFLVKRAFKRADIRNPLQVVTDGVEA
                     IHYLQGEGKYADRNLHPLPRLVVMDIKMPRKSGFEVLEWIKGDGTLKRIPVIIVSSSD
                     QPQDINRAYELGANAYMIKPMDFRSVEHLFQSITHYWGLECAKPEVEEEEQHA"
     gene            complement(1763..2701)
                     /locus_tag="VG754_13360"
     CDS             complement(1763..2701)
                     /locus_tag="VG754_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20251.1"
                     /translation="MDQVLYFFGSFVIALLQVMPLRWVAQLGRCGGEVVYWLDRRHRK
                     VALRNLTLCFQNTKSPEKIRALAHENFRRIGENFACSVKTAGMTEAQLKKVMEVNRDG
                     AFVAAGGGPQAECLIYATGHFGNFELFARLSWFIDEYKVAATYRGLRPNWLDRLVLGM
                     RTNSGSAMYERRTGAEELKRAMSEGGRLLVLFSDQSVRENGMELPFFGRPCQTSRAPA
                     VMAMRYKGTLFVPICYRTGLGRWRIEVGEPIPTHADGQRRSIEAITRDMNSAMEMAVE
                     RDPANWFWVHNRWKARRPQACCSSSSTSGLAHSSPQ"
     gene            2937..>3428
                     /locus_tag="VG754_13365"
     CDS             2937..>3428
                     /locus_tag="VG754_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWF20252.1"
                     /translation="MTQKTSVAAASNLFAEVSQTPAAQSLLRRLETGGALSCAGVSPA
                     AQPFLAVLLRHVFPERPIVVVADGLKTQESFQQDIQTWLGFAESKVQGPRFKAKDASS
                     HPPPSILHPQLFFYPAWEILPHESKLPHADIISERLETLVALSEHSALRTRHSALIVS
                     NVAA"
BASE COUNT          715 a         1046 c          918 g          749 t
ORIGIN      
        1 cggcgtgatg acgcttccgg ccgtgcccat gccgccgtcc aatcgcaaac caaatccgcc
       61 tgccgagcga taggtcagga tgcgcccgta gtccggcatg aatttgttgg cggggtcttc
      121 ggtcgtgatg cggcattgga ctgcgaaacc gttgcgggga atctcggatt gcaacggcaa
      181 atccaaatcc acgccgtgca gcgaatggcc ttgcgcgacg agaatttgcg agcgcacgag
      241 gtcaactccg gtaatggtct ccgtgaccgt gtgctcgacc tggatgcgcg ggttcatctc
      301 gatgaaaaac caggcgttcg tgtccagatc gagaaggaac tccaccgtcc cggcgttgtc
      361 gtaattgatt tcgcgcgcga tgcgcaccgc cgccgcgcac aattccgcgc gcacgctcgg
      421 ttccaatccg accgacggcg caatctccac caccttttga tgacgccgtt gcaccgagca
      481 atcgcgctcg tgcagatgca gcacgttgcc ctgtttgtcg ccgatcacct gcacctcgat
      541 gtgcttggcg cgcggaatat atttttccag aaagaccgcc ggattgccga atgcgcgtcc
      601 cgcttcattg cgcgcttcat ccagaagatc ggcaaggtcg gcggctttgt tgacgacgcg
      661 catcccgcgt ccgccgccgc caaaagccgc cttgatgatg agcggaaaac cgatttcctt
      721 ggcagccctc aaagcttcgg cgcggtcttc gatgggattt ggggtgcccg gcaaggtcgg
      781 cacgccgact ttttcggcca gggcgcgcgc ggcaactttg tcacccatca tgcggagcaa
      841 ctcaggccgc ggcccgacga aagtgattcc cgcatcggcg caagcctgcg cgaaatcgcc
      901 gttctcggaa agaaacccat aacccggatg gatgtaatcc acccctcttt ccctggccaa
      961 agccacgatg ccgggaatgt ccagatacgc gccaaccggg cccttgcctt cgccgacaag
     1021 atacgcttca tccgccttga accgatgcac cgacaggcgg tcttcctgcg cgtaaatggc
     1081 gacggtgcgc aaccccagtt ctgtggccgc gcggaagacg cggatggcta tttcactgcg
     1141 atttgcgaca agcagtttct tgagggtagg tgccggggcg ggcgtttccg gggtcgcggt
     1201 cgtatttcga gctttgctca ggttcgtcat ttgttctgtg ggcgagttta tagaccagca
     1261 aaccgcgctt ggcaaagaaa tgatggcggc caactggatt tttggcgtaa ctccggtaca
     1321 ttccaataga tagagggaac aaccaattat ttatgaacga cgcggatcaa tttactgttc
     1381 ttctggtcga ggacgatctc aacgatattt ttctggtcaa acgcgccttc aaacgcgccg
     1441 acatccgcaa tcccctgcaa gtcgtcaccg acggcgtgga agccatccat tatcttcagg
     1501 gcgagggcaa atatgccgac cgcaatttgc atccgttgcc gcgactggtc gtgatggaca
     1561 tcaagatgcc gcgcaaatca ggctttgagg tgctcgaatg gatcaagggt gacggcacgt
     1621 taaagcgcat tcccgtgatc atcgtttctt cctctgacca gccgcaagac atcaaccgcg
     1681 cctacgaact tggcgcgaac gcctacatga tcaagccgat ggattttcgc tccgttgagc
     1741 acctcttcca atccatcacc cattattggg gactggaatg cgccaagccc gaagtggaag
     1801 aggaagagca gcacgcctga ggcctccgcg ctttccagcg gttgtgcacc caaaaccagt
     1861 tggccgggtc gcgttccact gccatttcca ttgcgctgtt catgtcccgc gtgatcgctt
     1921 ctatggacct gcgctggccg tctgcatgag tgggaatcgg ctcgccaact tcaatccgcc
     1981 agcgtcccaa cccggtgcga taacagatcg gcacgaagag cgttcctttg taacgcatgg
     2041 ccatgaccgc cggcgcgcgc gaggtctgac aaggacgccc gaagaaaggc agttccattc
     2101 cattttcccg aacgctttgg tctgaaaaca aaaccaacag ccggccgccc tcgctcatcg
     2161 cgcgtttcaa ttcctccgct cccgtgcggc gttcgtacat ggcgctgccg gagttggtgc
     2221 gcatcccgag gaccagcctg tccagccaat tcggcctcaa gccgcggtaa gtcgcggcca
     2281 ccttgtattc gtcaatgaac caggagagcc gggcaaacaa ttcaaaattt ccgaagtggc
     2341 ccgtcgcata aatcaaacac tccgcttgcg gcccgccgcc tgccgcgaca aaagctccgt
     2401 cccgattgac ctccatgact ttttttaatt gggcctcggt catgccggcg gtcttgacgg
     2461 agcaggcgaa gttttctccg atgcggcgaa aattctcgtg ggcgagcgcg cggattttct
     2521 ccggtgactt cgtgttttga aaacagagtg tgaggtttcg caacgcgacc ttgcgatgcc
     2581 gccggtccaa ccaatagaca acttccccgc cacaacgccc taattgcgcc acccagcgca
     2641 acggcatcac ctgtaacaag gcgatcacga aggagccaaa gaaataaagg acttggtcca
     2701 tcaatatttc atgcctggcc cagggcagtt ttacccattt tgtgaatttg catgcgcacc
     2761 tgctgaattt tttttcccaa caacactacc ctcccgttat tgaacggcat tgtcgaggga
     2821 aaagggaaat gaatgtgctg aaatcgagtt gcgagcgaag ccattgttcg agatttcctc
     2881 cacgcgccgc ccagttgact tgacgtccct ttcgaagcct gcgattttta tcaggcttga
     2941 cgcagaaaac atcagttgcg gcggcaagca atttgttcgc tgaggtttcg caaactcccg
     3001 ctgcgcagtc cctgttgcgg cgattggaga caggcggcgc attgtcttgc gccggggtga
     3061 gcccagccgc gcagccgttt ctcgccgttc tattgcgcca tgtttttcct gaaagaccga
     3121 tagttgttgt cgccgatggc ttgaaaacgc aggaaagttt tcagcaggac atccagacct
     3181 ggctcggctt tgccgagtcc aaagttcaag gtccaaggtt caaagccaaa gacgcttcca
     3241 gccatcctcc accctccatc ctccatcctc aattattttt ctaccccgcc tgggaaattt
     3301 tgccgcacga atccaagctg ccgcacgccg acatcatcag cgaacgcctg gaaacgctcg
     3361 ttgcgctttc tgagcactcc gcactccgca ctcggcactc cgcacttatt gtctccaatg
     3421 ttgccgcc
//
LOCUS       DASZMM010000559         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4923506, whole genome shotgun sequence.
ACCESSION   DASZMM010000559 DASZMM010000000
VERSION     DASZMM010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4923506"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VG754_13370"
     CDS             complement(<1..488)
                     /locus_tag="VG754_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20253.1"
                     /translation="MNKKLHSPGAARGLNIEKTSKTIAAVLSLLCLLAVMVSTSQAGV
                     ALKLSYDGIGNNGISNNYVTNLTTDPSFPSSPDPSYSETLTNGLTITPTGIHNLGTWT
                     RGFIEAPETGQYTFWMASTDEGQLWLSTNESPANLVELADNPLPITYGSYLPVNQSPK
                     IS"
     gene            complement(534..1373)
                     /locus_tag="VG754_13375"
     CDS             complement(534..1373)
                     /locus_tag="VG754_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF20254.1"
                     /translation="MKKYNLPRRTWRIRGFTLIELLVVVAIIAILAGLLLPALARAKA
                     KGKRISCMNNLRQISIFIQMYTDDNQDIFPPHRNNLQAPGNTSSVPTDWWGTTIIGYT
                     QNMSNLFHCPVLVGAIPLQPPGQAPGLPWMWSFTVDFVGYGYNGYFLGLWPYAAGSVT
                     VDGVTFILPQSFKRSAIVNPSSSLCIGDKDPTYNGAGQQGYWSSSLWWPNACMDPADT
                     GSIHEGIDPYRHLGEGNMMFNDGHAETRKSQDINPPRDPGENTPNSIVNCRYWDPLQR
                     SMK"
     gene            1702..2850
                     /locus_tag="VG754_13380"
     CDS             1702..2850
                     /locus_tag="VG754_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HWF20255.1"
                     /translation="MTKRRKVALLIETSNAYARGLLQGVVRYIREHSPWSFYLPEQGR
                     GDVPPGWLAHWRGDGIIARIENKDIANAVADSGLPAVDVSAARMLPSLPWVETDDAHI
                     SRVAAEHLLDRGFKHFGYCGDARFNWSNWRAEHFEKCIITAKHACHHYRPAIALNAPL
                     EEQVSEIAEWLRNLPKPVAVMACYDIRGQQVLDACRNAGLAVPDEVAVIGVDNDELLC
                     DLSSPPLSSVMPNTQRTGYEAAALLDRMMAGRPVAPKGHFISPLGVATRQSTDTLAIE
                     DRVIARAVRYIREHAFEQINVERVLKAVPLSRRLFESRFKKLLGHTPHEEIIRVRMNR
                     VKEMLTETDLSLADIAERTGFEHVEYLSAAFKKREGLPPNRYRAQNQK"
     gene            complement(2975..3289)
                     /locus_tag="VG754_13385"
     CDS             complement(2975..3289)
                     /locus_tag="VG754_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWF20256.1"
                     /translation="MTENAAGQIKHLQDTQSENTGKHLRIYVEQGGCSGKQYGMVFDE
                     RRDGDLITDMHGVSVLVDPVSADHVRGSVVDFNDSLTGGGFKISNPNARQSCGCGKSF
                     EA"
     gene            complement(3418..>4015)
                     /locus_tag="VG754_13390"
     CDS             complement(3418..>4015)
                     /locus_tag="VG754_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20257.1"
                     /translation="DPSTSDSNKFSTAIDVFHHIATDSIYINTPMAARAWGQLGSCYY
                     KMNGDASTQGITNAALRDVALQRYERASEYFQNAITNAQADVTTRSLAEIRLGQTQAA
                     MAALQPEQRQKLLEDALGYFFSVFYEDNLRDNEQPDASCLKEAGLEAGRLAGEMSDWK
                     QAKQIYQKLLKLMPPLQSAIEKKIAYAEAQENLPAEKK"
BASE COUNT          894 a         1050 c         1094 g          977 t
ORIGIN      
        1 aatgatatct tgggtgattg gttcacggga agataagaac cgtaagtgat gggcagcggg
       61 ttgtcagcca gttcaaccag gttggcggga gattcattcg tgctgagcca caactggcct
      121 tcgtccgtgc tcgccatcca aaaggtgtat tgaccggttt cgggagcctc gatgaaaccg
      181 cgtgtccaag tgccgagatt gtggatccct gtcggtgtga ttgtcagccc attggtcaaa
      241 gtctcggaat agctgggatc gggactgctc ggaaaacttg gatcagtggt caggttggtg
      301 acataattgt tggaaatgcc gttattgcca atgccatcat aagagagctt cagggccacg
      361 cccgcttgcg atgttgaaac catcacggca agcaggcaga gaagggacaa gacagcagca
      421 atcgtcttgc ttgttttttc gatgttaagg ccacgcgccg cgcctggtga atggagcttt
      481 ttattcatgt tggttgagtg gttggttgtt ggtaaatgat gctgcggaaa atttcatttc
      541 atggagcgct gcaaaggatc ccaataacga caattgacga tgctgttggg cgtattctcg
      601 cccgggtccc gcgggggatt gatgtcctgg cttttccggg tctcagcgtg cccgtcatta
      661 aacatcatat ttccttcgcc caaatgccgg taaggatcga ttccttcatg aatgctgccg
      721 gtgtcggctg ggtccatgca tgcattgggc caccaaaggc tggaagacca atatccttgc
      781 tggccagcgc cgttatacgt tgggtcctta tctccgatgc acaggctgga cgacggattg
      841 acaatggcac tacgtttgaa agattgggga aggatgaatg tgactccgtc cactgtgacg
      901 gagcctgcgg catacggcca aaggcccaaa aagtagccgt tatatccgta accgacaaaa
      961 tcaacggtaa aactccacat ccatggcaga ccgggagctt ggccgggagg ctgaagcggt
     1021 attgcgccga ccaagaccgg gcaatggaac aggttggaca tgttttgcgt gtagccgatg
     1081 atggtagtgc cccaccaatc agttggtaca gaagatgtat tgccgggggc ctgcaaattg
     1141 ttccgatgcg gaggaaatat gtcctgattg tcatccgtgt acatctgtat gaagatcgat
     1201 atttgccgca gattgttcat gcaggaaatg cgcttccctt tggccttcgc tcgcgccaat
     1261 gcgggaagga gcagcccggc gagtatcgcg atgatcgcca cgacaaccag cagttcaata
     1321 agcgtgaacc cgcgaatccg ccacgtgcgc cggggcaaat tatatttttt gatgtcaggc
     1381 agagcttgcg ctgcctgagc attcgcgccc gggaaagaca cttctctttg gcgtagaatg
     1441 tgatgcattt aaactgggtt cattcaaaga aaccacaact tgccggacac gggaatgagc
     1501 tttcacgcaa ttcttttgag atttttaacc gacaaagcgg ttgactcaac aggttgttct
     1561 tctgatattt gccgggaagc cagcacgatc ataaaaccgc gcgccgtttt tcaaaagcgc
     1621 gggcgggcac ggcaaaaata tatggtccga gggcgcgagg aaaagttcca ggctcgcacg
     1681 gttgccacgg ttcgaataat tatgaccaaa cgtcgcaaag tcgccttatt gatagaaact
     1741 tccaacgcct atgcgcgcgg gctgctccaa ggtgttgtgc gatacatccg cgagcattct
     1801 ccctggtcgt tttatcttcc tgaacaaggc cggggcgacg tgccgcctgg ttggttggcg
     1861 cattggcgag gcgacgggat catcgctcgg attgaaaata aggacatcgc caatgcagtg
     1921 gccgattcgg gccttccggc ggtggatgta agcgccgccc gaatgctgcc atcgctgcca
     1981 tgggtcgaaa ccgatgacgc gcacatttcc cgcgtggcgg ctgaacatct gttggatcgc
     2041 ggtttcaagc actttggcta ttgcggagac gcgcgcttta attggtccaa ctggcgggcc
     2101 gaacactttg aaaaatgtat cattaccgcc aagcacgcct gccaccatta tcgtccggcc
     2161 attgcattga acgcgcctct cgaagagcag gtttcggaaa tcgccgaatg gctccgcaat
     2221 ctccccaaac cagttgctgt gatggcgtgt tacgacattc gcggccagca ggtgctggac
     2281 gcctgccgca atgccggctt ggctgtgccg gatgaggttg cagtgattgg tgttgataat
     2341 gacgaattgc tatgcgacct ctcctcgccg ccactttcca gtgtgatgcc aaacactcag
     2401 cgcacgggtt atgaagcggc ggcgctgctt gatcggatga tggccggccg gcccgtggct
     2461 cccaagggac attttatttc ccctctcggc gtggccacgc gccaatcaac cgataccctc
     2521 gccatcgagg atcgagtcat cgcccgggcc gtgcgctaca ttcgtgaaca cgcattcgaa
     2581 caaattaatg ttgaacgcgt attaaaggcc gtccccttgt ctcgccgtct cttcgagagt
     2641 cgttttaaga agctcttggg ccacacgccg catgaagaaa tcattcgcgt tcgaatgaat
     2701 cgcgtcaaag aaatgttgac ggaaaccgat ctgtcactgg cggacattgc cgagcgcaca
     2761 ggattcgagc atgtcgaata cctcagtgcc gcctttaaaa aacgcgaggg tctgccgccc
     2821 aatcgttatc gcgctcaaaa tcaaaaataa gagaagatgt tgtcggactc gctccgcaaa
     2881 tacgcgcggg cggctaactc ttgttggaat gaaataacaa agccgtttcg catcacacga
     2941 aacggcgttg ttcagcctgc gcagatgtgt cttgtcaggc ctcaaatgat ttcccgcaac
     3001 cgcaactctg gcgggcattg ggatttgaaa tcttgaatcc gcctccggtc aaactgtcat
     3061 tgaaatccac cacagagccg cggacgtgat cggcgctgac aggatccact aaaacagaaa
     3121 caccatgcat gtccgtgata agatcaccat cacgcctttc gtcaaatacc attccgtatt
     3181 gcttgccgga acatccaccc tgttccacat aaatgcggag atgtttgcca gtattttcag
     3241 actgcgtgtc ttgcagatgc ttgatctgtc ccgcagcatt ttcagtcacc gaaatgactg
     3301 gctcagttga tgcgacgttg ttcatgtttg caagttaaag cagtttaatt gcagtttcgg
     3361 cccctgtcaa atgggtctgg ctggcatgtc cccggatgcc acatccgcga gatttgctca
     3421 cttcttctct gcgggaaggt tttcctgcgc ttcggcgtag gcgatctttt tctcgatggc
     3481 actttgcagc ggtggcatca gtttcaacag cttttgatag atttgcttcg cctgcttcca
     3541 atcgctcatt tctcccgcaa gccttccggc ttcaaggccg gcttccttga gacatgaagc
     3601 gtccggttgc tcgttgtccc gcaaattgtc ctcataaaaa acgctgaaga aatatccgag
     3661 cgcatcctca agcagtttct ggcgttgttc gggctgcaac gccgccattg ccgcctgcgt
     3721 ttgcccaagc cggatttccg ccaggctccg cgtcgtcacg tccgcttgcg cgtttgtgat
     3781 ggcgttctgg aaatattcgc tcgctcgttc gtagcgttgc agagcgacgt cacgcaacgc
     3841 agcgttggtg ataccctgcg ttgaggcatc tccattcatc ttataataac aactccccaa
     3901 ttggccccac gcccgagcgg ccatgggcgt gttgatgtaa atggaatcgg tcgcaatatg
     3961 gtggaaaacg tcgattgctg tgctgaattt gttcgaatcg ctcgtcgaag gatcc
//
LOCUS       DASZMM010000560        17985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2725960, whole genome shotgun sequence.
ACCESSION   DASZMM010000560 DASZMM010000000
VERSION     DASZMM010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 17985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17985
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2725960"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VG754_13395"
     CDS             complement(<1..1383)
                     /locus_tag="VG754_13395"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWF20258.1"
                     /translation="MNRIVGLETEYGCLTNTPAGSFIAVERVRDWIFGREEYGLIDVH
                     HRDWDEPPGNGGFLFNGGRVYIDMGHLEYCTPECVSLIDILRYDRAGDALTLHAVKSL
                     GLAGDVSFIRNNIDHYTGATFGCHENYLVRRSAPLTETNVLSLLTFLTLRLLFTGAGR
                     VGTTLAAEWRGEVSRPGTDGGFQMSQRADYINNDLYEWVQFNRSIINTRDEPLGDPRK
                     YRRLHLLHGDTNVLPATLLLKVGTTSLMLDLLEINHLPKVVLADAVKTFRALSHQTEG
                     PWEVRLADGRVMDAVELLERFFSAAWAEFHGRDIETDILLDVWGSTLKALSAEPQALI
                     GKVDWITKQWLLRKFMEQEKLEWEDPWLKAQDLEFHHIDPARSLGVALDQPPAPWALT
                     PKMIDIAVMEPPVNSRAQARSRIMRLLKEHAPPYSIDWEIVGVEGINPLNLLNPFDPS
                     PPELKTWYTEF"
     gene            complement(1514..2254)
                     /locus_tag="VG754_13400"
     CDS             complement(1514..2254)
                     /locus_tag="VG754_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20259.1"
                     /translation="MTEEPYRWLEAVSNRREYVREQIKGGSPALMASLPDGILLIGVG
                     GGQSKVFELFDRHALAGLGHPADIEKIRQAAIDAAHTEAFTRAPEDVSLRRLVGFGLS
                     PQLKTNFEQIFTAPFLVELLLAEVGAERGKDLLLRLHFDGAFQFQNSGVMIAASQPEP
                     EAEAKAWLERTLADQSDRAAVAELMLQAWWCLLENKHWGAQFPNETDRKEGWRKATQG
                     RVVEIGWLARKSSRQAHYESLTLAQLGL"
     gene            complement(2263..3039)
                     /locus_tag="VG754_13405"
     CDS             complement(2263..3039)
                     /locus_tag="VG754_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HWF20260.1"
                     /translation="MSSESPTLAGDFFSLLAAQKLAPWRGEVSSAAPVQTQATTVFAF
                     HFSEGVLMAGDRRATAGNVIVTDRVDKVIEIDATSLLAIAGVPATAFEMARVLQTSFE
                     YYRRSQLQPLSLPAKVRALARLLRENLPMTMQGIGIVAPLFASLDHAITPAKPQIYFY
                     DPLGAQFQAVGHVASGSGSGTIRSIMSFQEQYGQPPPSKMKLKEAVRFALRLLMVASE
                     FDSATGGVNPETNTFATIRVLRSTGVEAVTDEQQSEFLKP"
     gene            complement(3036..3206)
                     /locus_tag="VG754_13410"
     CDS             complement(3036..3206)
                     /locus_tag="VG754_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HWF20261.1"
                     /translation="MPDQAQKTRPMGPGPSGGGDGPSSPKVEKPNTDELLKRMRKVDP
                     DQAKRYRQRTGQ"
     gene            complement(3229..4752)
                     /locus_tag="VG754_13415"
     CDS             complement(3229..4752)
                     /locus_tag="VG754_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWF20262.1"
                     /translation="MTLPPLIQFGIETEIGIARDNGEDMDVVSESIALVRSVVEPGVL
                     MRWDYGSEDPHADMRGFHVQELRQDTDESNYYEEDSQRELTYVEIKSDLVLGNGARFY
                     NDHAHPEYCTPECSTLDELIAQDRAGERILMACAHQLSIQRGQTVRLYKNNTDFRGHS
                     YGCHENYLLPRSLPWSQLAQGVQAFLVTRQICAGAGKFAIEEEDRFVSSDFQISQRSD
                     FFSELQSVDTMQRRPIVNTRDEPHANPRLYRRFHVILGDANMSPFATRLKIGTTALVL
                     EVLARDPQRIWPQLADPLGALQSISRDPAFRWEVKLTDGRTTTALELQREYLRVVKLV
                     CDLSLPAKAAMVKDWEGVLNDLETDYMRCRNRLDWVAKLALVREFQAAQNIAPDDPWL
                     QSLDLEYHRLDLGEGLYYALEQAGSILGVPEESAIRWAVREPPRTTRALVRGKCIQKF
                     ASAVEAAQWDHITLQAGGESIKISLMDLFAPEEILRYGKIIEGARSPEDLRTLKSVS"
     gene            complement(4979..6580)
                     /locus_tag="VG754_13420"
     CDS             complement(4979..6580)
                     /locus_tag="VG754_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF20263.1"
                     /translation="MEALSALQLVDLIAGKLPPQHSSLMDLIYLRDRIAAIEEMNDQA
                     RQALEKLDEIVEKLRSPAFRVGTFLTAVENDKAHVCVGGTDYVCRVDPKIPLNSLQMG
                     QRVLCNEAFAVVQGLGFDRNGPIVRIDELLSDGRLRIGQEAGLMSLVLLRSALLVKEK
                     LKPGMEVRLDVNQRVALEVIGMGKRIERSLETVTELPWSAIGGQSEAVQAIRDTIELP
                     FLHRDLFKRFEHTVPKGFLLHGPPGCGKTLLGKATAYNLRQQIKAQTGVDHPEFFLHV
                     KGPEILNMWVGESERQVRDLFIQCRERARDGALAFLFIDEAESILGTRRSGRYSSILS
                     TLVPMFCTEMDGLEPLQNVVVILASNRADLIDPAILRPGRIDRKIKVNRPDKAGAQAI
                     YEIYLRESLPLGEPRPDLAKAVTEEHFAHSPTNQFLEIVFRSGKKDFLYRGDLASGAI
                     IAAVVERAKSFAIKRSIQTGQETNLTREDLIHALHREYEENDLFPPTDVTEDWLKLTD
                     FDPENVIKLGPVRPVRKDGHSSGVV"
     gene            complement(6958..7239)
                     /locus_tag="VG754_13425"
     CDS             complement(6958..7239)
                     /locus_tag="VG754_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20264.1"
                     /translation="METETRKGNGHGMTLEKLMQDLKVVVDDGQELLKGGAGQLRIKA
                     VAGAKATDETIRRNPYPSMAIIFGLGVALGILGFSLLQGGAEEFGEEQD"
     gene            complement(7395..8579)
                     /locus_tag="VG754_13430"
     CDS             complement(7395..8579)
                     /locus_tag="VG754_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF20265.1"
                     /translation="MKNCALRILHLEDDPFDGELLRRILTHEGLVRDWVRVESAKAFT
                     TALNEGLFDLILSDYTLPSFNGIEALALARQHHADTPFIFVSGTIDEDVAVESLKNGA
                     ADYVFKNRLSRLLPAVRRALKEVEDHAESRRAEEAMRESEHKYRQVFESMSEAAFLVD
                     LPTGRVLDANQRAESFLGRARSEIIGAKHECFHVHGKFEAYRKVLADVLAHNTRAEVE
                     SEIICKDGRTFPVQVRASTLELYGRHLVLCLCRDITKRKQAEDQFRERSQLLDLLPEA
                     ILVRDLDGRIQYWNEAATRLYGWSCEEALGRNVTELLYHDTSEFQVANQAILKNGQWQ
                     GDLHQVDRANHEILVHSRCTLLRNERDEPKSVLLVNSPIGEPAVNRLESRGKTDVLHG
                     AN"
     gene            complement(8579..11482)
                     /locus_tag="VG754_13435"
     CDS             complement(8579..11482)
                     /locus_tag="VG754_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HWF20266.1"
                     /translation="MSILLAHPRPEYPRPDRQRGTLEGIDWLNLNGPWQFRFDRYRRG
                     LEQGWHEVEQPEWREQIVVPFCWESLAAWGEGDAAGNENYYATRVFLNPLEVDRSNHR
                     SAQRYEVGWYRRMVEIPEGEEWEGKRVILTIGAADFFTDCWCNGQHLGHHEGGYTPFE
                     FDLTDALETSADGKREAVIVLRVEDPMDNHEQPVGKQWRWYTTISGIWQTVYIEPRDA
                     SFLRLFRIYTDIDAGTARFEIDCENAADDCVVTAEILPPGHSPQKAEFPVRDGKVKEA
                     VKVNPLLLWNPNEPNLYKVILRLQRAGQTLDTVRTYFGMRKIDFAPGEPPESPVLLRL
                     NGQPRYLRGALHQSYYPEGVYTAGDVATFKNDIAYAKKVGFNFLRIHIKIDDPLLLYH
                     ADNMGMLLMADIPNFGEGGDTPLGRRRFEIMMREAIERDFNHPSIFAWCIFNETWGFG
                     GQAEFADIIKSLNPKDRRRGQKAPGAVSARSNGGNGGGVVTATMTAPVKAPRAKMANL
                     SSHAWVQMMWELAKKLDPTRLIEDMSVVHWEHLDYFAHGDTDINSWHFYIDEYERARE
                     HIAKVVKSTFPGSNFNYVPGFAHKGQPLINSEYGGVGALDGDRDISWSFKFLTNELRR
                     YGQISAYIFTELHDVEWEHNGFLNYDRTPKEFGYDPAMINESDTIPIDAPPISRVRPG
                     ETVCLPVASSHFSSRKTRNISLRWKMDGIDMRGHLHQSVTCGCVPIEFPHRRVVPAHT
                     LELRMPSEPMLCTLWLEACTEEGTVVASNFLQYFVSEVYPPHREEGPRAMVLRGTPGD
                     WAAGEWSGGGMSERARAGAEDSCFGMGHGFFEWVLPLGGADVTKARRIRVLCEASSHR
                     IDTPQTDADIFPTTLQMFLNGIQVYGAWIRNHPHDARGSLSYLRGGLGAYGYLVHAFA
                     EADSVRQIALHVMDDSLRLRCVVPEDALAQGGLTIYGAECGRYPVAPTVIIEW"
     gene            complement(11657..12595)
                     /locus_tag="VG754_13440"
     CDS             complement(11657..12595)
                     /locus_tag="VG754_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20267.1"
                     /translation="MRCKTWILALACLAGICIAVDRVFTEIIIFRDPSQDGARIKHLC
                     EDNSEDIPIFGPSKAHGNYSPNDMGIKAYNYGFNGASYEVVDTFLQIELAKPRTTPII
                     IELQHRDTGILGEKSQFIPFASDPRFQPLLKKFNAMSWRYHFPGIRYFGYYDFILSTY
                     LSGCFHVSKMSQGFGELVRTPPFNRAQLDQYIRERLAYQNGYYPDEDQNRRFIAHIKG
                     HPQRLFFLVVSPYHPSYYANFENEDKFEAYKKELAAIPNVVVIDWGKLDYPDEYFLDT
                     LHLRRGAAAIFSRQLGNKIREVLEKRKQQPPNTAAN"
     gene            complement(12601..14001)
                     /locus_tag="VG754_13445"
     CDS             complement(12601..14001)
                     /locus_tag="VG754_13445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HWF20268.1"
                     /translation="MGLDSAIFFLFLAWVAILQAYLPERGRIALLLCASLAFYAFSSV
                     SYLFLLLMLCAINYWLLRGLSATEDPRRRDWFFAGAVVANLAVLISFKYASELLSGVT
                     AKFGWPGEGGKVARLAVPLGLSYFTFQMLSCVTDVYRQSWRMEKGFSRFAVFGLFFPQ
                     ITSGPIPRAPGLLPQLSCCPTAEDRLAGIRLIAYGLFKKYVVANRLSLYVSGVFDHPE
                     TANTTSAFLTVCFSALQLYADFSGYVDIAIGSARLLGIRLEPNFDRPYLSTSMTELWR
                     RWHMSLSFWLRDYLYMPLVIRIRALGKVGIALALIITFAICGIWHAATWPFLLFGIAQ
                     GVALSAEFLTKPWRTKWLKRAPKRIVIMVASLYTLGFFALSQVLFRSSNLSQAGTVFS
                     RLFHLRMSGSLNDLLGARPYNFVWNCMALAAWFAVATLRRRTSDRWTPWFAVLCGTLV
                     LLLGSLGSAHFIYAAF"
     gene            complement(14060..14299)
                     /locus_tag="VG754_13450"
     CDS             complement(14060..14299)
                     /locus_tag="VG754_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWF20269.1"
                     /translation="MNDTLWNDLTGIFKDVLDNDSLVLEEKTTAKDVDGWDSITHVLI
                     VVAVEKKFRVKFTAGEIQQLQKVGDLAALIRRKQS"
     gene            complement(14304..16058)
                     /locus_tag="VG754_13455"
     CDS             complement(14304..16058)
                     /locus_tag="VG754_13455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HWF20270.1"
                     /translation="MTDLPELTTLLKLARRPRADDAAILRAALLADTSSQLLAGALRG
                     YARHLGFELDLFEGDFDQIEPLVFDPASALHSFRPETVIVYVAAERTRDKFGTLSIAE
                     RGDFAVNFSGRVRALHKSLSHSGCQTLFFNLADPGDGVFGNYGNKVHWSLQNQIRFCN
                     AELARLAGEADDFHVCDLSALQSELGRGALHDPKLYYTSKFALAPGVLPLVARNVVKM
                     LAARKGSFVKCVILDLDNTLWGGVIGDDGLERIQIGDLGLGPAFSAFQSWIKQLRDRG
                     IVLAVCSKNDEANAREPFLKHPDMVLRLEDIAVFMANWDDKAANIRCIKKIIDVDYGA
                     MMFLDDNPAERQLVRESFPTMQVPELPADPSEYVAYLTSLNPFETASYTAQDAQRTAE
                     YQTEALRHVEREKFINLPDFLRSLEMRMLAQPFLPFTFPRVAQLTQRSNQFNLRTVRY
                     AEKEIAALAASPKHVTLTFSLKDRFGDNGLISVVILEILGNEFFVDTWLMSCRVLGRG
                     VEEFVLNAIMAKARERGIKRIIGEYLPTLKNKMVENHYLKMGFAVEADNRWTLETDGY
                     LERSTCIIPEETMESRNP"
     gene            16230..17456
                     /locus_tag="VG754_13460"
     CDS             16230..17456
                     /locus_tag="VG754_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20271.1"
                     /translation="MSKLRHCFFYLIFAGILCPLVAVAQIDPAKRELVQIGYNQSLIG
                     QAPLSAYAFYYYNKPDFLNESNLTLRLAVAPVYVDSELGISHALGPDTDVGIGLAGGG
                     FADSYYEVRQGKYLKAESFDGDGVTGSGSIYHLFNPGQRIPLNGLFRVEGHYADYIRD
                     DTAPNFVLPKDMTDFNIRTGFRFGGKEPVMVPDMAMELSLWYEGQFRLNSGDYGFNND
                     RHVNSISHLFWGRALLAYTLPESKQNFLVSVTAGDSIQADRFSAYRIGGFLPLAAEFP
                     LSLPGYFYQELSATRFALFNVNYTVPIDAEKRFAFTAIASTAIMEYLPGLEQPGHWNS
                     GVGGGLSYHSHSDAWRVLVDYGYGFNAIRDNGRGGQTIGILLQINLERTHPQYVVPGS
                     GSGILRGLGDFTHSIF"
BASE COUNT         4043 a         5355 c         4649 g         3938 t
ORIGIN      
        1 gaattcggtg taccacgttt tcaattccgg cggcgaagga tcgaagggat tgagcaggtt
       61 cagcggattg atgccttcca cgccgacaat ttcccaatca atggaatagg gcggggcgtg
      121 ttctttgaga aggcgcatga tgcgtgaacg cgcttgtgcg cgggaattca ccggcggttc
      181 catgacagcg atatcaatca tcttgggggt caacgcccac ggagcaggcg gttggtcgag
      241 tgcgacaccc agactgcgcg cgggatctat gtggtgaaat tccaagtcct gcgccttcaa
      301 ccagggatcc tcccattcca atttttcctg ctccatgaat ttgcgcagca gccattgttt
      361 tgtgatccaa tccaccttgc cgatcaacgc ctgcggttcg gctgaaagcg ctttcaatgt
      421 cgagccccaa acatcgagca aaatgtccgt ctcgatgtcg cgcccgtgaa actccgccca
      481 tgccgcgctg aaaaaccgtt cgagcaattc caccgcgtcc atcacccggc catccgcaag
      541 tcgcacttcc cacgggcctt cggtttgatg cgacagcgcg cggaaagttt tcacagcgtc
      601 cgcgaggacc accttgggca aatgattaat ttcgagcaaa tcgagcatca acgacgtggt
      661 gccgactttc aaaagcagtg tcgcaggaag aacattggta tcgccgtgca acaaatggag
      721 acggcgatat ttgcgcgggt caccgagcgg ttcatcgcgg gtgttgatga tggagcgatt
      781 gaactgcacc cattcgtaaa gatcgttgtt gatgtaatcg gcgcgttggc tcatctggaa
      841 gccgccgtcg gtccccggcc ggctcacttc gccgcgccat tcggcggcaa gggttgtgcc
      901 gacacggccc gcacccgtga aaagcaggcg caaagtaaga aacgtcagta acgagagcac
      961 gttagtctcg gtcaacggcg cggagcggcg gacgagataa ttttcgtggc agccgaacgt
     1021 cgcgccggtg tagtggtcaa tgttgttgcg gatgaaactt acatcaccgg caagaccaag
     1081 cgatttcaca gcatggaggg tgagcgcgtc gccagcgcgg tcataacgaa gaatatcaat
     1141 cagcgatacg cattccgggg tgcaatactc caaatggccc atgtcaatat agacccgccc
     1201 gccgttgaac agaaaaccgc cgttgcccgg cggttcgtcc caatcccggt gatgcacgtc
     1261 gatcaggccg tattcttcgc ggccgaaaat ccaatcgcgc acgcgttcca cggcgatgaa
     1321 cgagcccgcg ggcgtgtttg tgaggcagcc atattcagtc tccaatccga caatgcgatt
     1381 cataaacaac tcaccctgat aatacttctc cctttttcct ctttactctc ggctttttcg
     1441 cgccctgaat ccagtagaat taatggggaa ctttttgcag caagccttcg ggacggacag
     1501 gagttacacg tcatcacaaa cccaattgtg ccaatgtcag gctttcatag tgtgcttgac
     1561 gggacgattt tcgcgccagc cagccaattt caacaacccg gccctgcgtc gccttccgcc
     1621 agccttcctt gcggtcggtt tcattcggaa attgggcgcc ccaatgtttg ttttccaaaa
     1681 gacaccacca cgcctgcaac atcaattcgg ccaccgccgc acgatccgac tggtccgcga
     1741 gagtccgttc gagccacgct ttggcttcgg cttccggttc cggctggctt gccgcaatca
     1801 tcacgccgct gttctgaaat tgaaatgcgc cgtcaaaatg caatcgtaac aataaatcct
     1861 tcccgcgttc cgcaccgact tccgccagca gcaattccac cagaaacggc gctgtaaaaa
     1921 tctgctcgaa attcgttttc aattgcgggc tcaagccaaa tccaaccagt cgcctcaagc
     1981 tcacgtcctc cggcgcgcgc gtgaacgcct cggtgtgtgc ggcatcaatc gcagcctgcc
     2041 gaattttttc gatgtccgcc ggatgaccca acccagccag cgcatgccgg tcaaacaatt
     2101 caaaaacctt cgattgtccg ccgcccacgc cgataagcaa aattccatcc ggcaaactcg
     2161 ccatgagcgc cggcgagccg ccttttatct gctcgcggac gtattcgcgg cggttggaca
     2221 ccgcctccag ccaccgatat ggttcttcag tcatgcttgc tgtcagggtt tcaaaaattc
     2281 cgattgctgc tcgtcggtca cggcttcaac gccggtgctg cgcaaaacgc ggatggtcgc
     2341 aaaagtattt gtctcaggat ttacgccgcc ggtggctgaa tcaaattccg acgccaccat
     2401 caacaatcgc aaggcgaacc gcaccgcctc cttcagtttc atcttggagg gtggcggctg
     2461 gccgtattgt tcctgaaaac tcatgatgct gcgaatggtg cccgagccgg aaccggaggc
     2521 gacatgtccg acagcctgga actgcgcgcc gagcggatcg taaaaataga tttgcggctt
     2581 ggctggcgtg atcgcatggt ccaagctggc aaacaacggt gcaacgattc cgatgccctg
     2641 catcgtcatg ggaagatttt cgcgcagcaa gcgcgccaat gctcgaacct tggcgggcag
     2701 gctgagaggt tgcaactggc tgcggcgata atattcgaag gaagtttgca ggacgcgcgc
     2761 catttcaaat gccgttgcgg gcacgcctgc gatggccagc aacgaagtcg cgtcaatctc
     2821 aatcaccttg tccacgcggt ctgtgacgat gacattgccg gcggttgcgc gccggtcgcc
     2881 cgccatcaaa acgccttcac tgaaatggaa ggcgaagacc gtcgtggcct gggtctgcac
     2941 cggcgccgca gacgaaactt ctccgcgcca aggcgcgagt ttttgcgcgg cgagcaggga
     3001 aaaaaagtcc cctgccagcg tcggcgattc gctgctcatt ggccggtgcg ttggcgatag
     3061 cgtttggcct gatcggggtc caccttgcgc atccgtttca ggagttcgtc cgtgttgggc
     3121 ttctccactt tcggcgagct gggaccgtca ccgccgccac tcgggccggg ccccatgggc
     3181 cgcgtttttt gtgcttggtc tggcataagt tcctgcattg ttggtttatt atgacactga
     3241 tttgagcgtt cgcaaatcct ccggcgaacg cgcaccctca attattttac cataccgcaa
     3301 aatctcctcc ggcgcgaaca aatccatgag cgaaatcttg atggactcgc cgcccgcttg
     3361 caaggtgata tgatcccatt gcgccgcctc gactgccgag gcaaatttct gaatgcactt
     3421 gcccctcacc aatgcccgcg ttgtgcgcgg cggttcgcgt acggcccagc ggatggccga
     3481 ttcctccggc acacccagaa tcgaacccgc ctgctcaagg gcgtaataca aaccctcgcc
     3541 gaggtcaagc cggtgatatt ccaaatccaa actttgcagc cagggatcgt cgggcgcgat
     3601 gttttgcgcg gcttggaatt cccgcacaag cgctagtttg gcgacccaat caagccggtt
     3661 gcggcagcgc atgtaatcgg tttccaaatc attcaacaca ccttcccaat ctttcaccat
     3721 cgcggccttg gccgggaggg acaaatcgca aaccaatttc accactcgca aatattcgcg
     3781 ttgcaactcc aatgccgttg ttgtccgccc atcggtcagt ttcacctccc agcggaacgc
     3841 cgggtcgcgc gaaatcgact gcaacgcgcc caatgggtcg gccagttgcg gccagatgcg
     3901 ttgcggatcg cgcgcgagca cttccaggac aagcgccgtc gtgccgatct tcaagcgcgt
     3961 ggcgaacgga gacatattgg catcgccaag aatgacgtgg aaacggcggt agagccgcgg
     4021 attggcgtgg ggttcgtcgc gggtgttgac gatgggccgg cgctgcatgg tatccacgct
     4081 ctggagttca ctgaaaaaat cgctgcgctg cgaaatctga aaatcggagg aaacgaaacg
     4141 gtcctcttct tcgatcgcga acttgcccgc accggcgcaa atctggcgcg tcacaagaaa
     4201 cgcctgaacc ccctgcgcga gctggctcca cggaagcgag cgcggcagca ggtaattttc
     4261 atggcaacca tagctatgac cgcgaaaatc ggtgttgttc ttgtagaggc ggacagtctg
     4321 gccgcgttgg atggagagct ggtgcgcgca ggccatcaaa atgcgttcgc cagcccggtc
     4381 ctgcgcgatc aattcgtcca aagtgctgca ttccggtgtg caatattcgg ggtgggcatg
     4441 gtcgttgtaa aagcgcgcgc cattgccaag cacaaggtcg ctcttgattt cgacgtaagt
     4501 gagttcacgc tgagaatcct cctcgtaata attggattca tcagtgtcct gccgcaattc
     4561 ctgcacgtgg aaaccgcgca tatcagcgtg gggatcttca ctgccgtagt cccaccgcat
     4621 caacacgccc ggctcaacca cgctgcgcac caaggcgatt gattccgaca ccacatccat
     4681 gtcttcgcca ttgtcccggg cgatgccgat ctccgtttca attccaaact gaatgagcgg
     4741 tggcagcgtc atcttccttt atcacccgaa gctccgtgca atgtgcggac aaaacatact
     4801 gcgtgaaact gctacacatt ttgagttaac gcaacggaga aaaagcacag attttaaact
     4861 ccggaaaaaa ttcaggcgcc gggcgcaatt ttcttgttcg cgcttggcgc ctgtgagttg
     4921 aagcgtggct gaatttgaaa acttgaacgg cttttgcttc acaacatctg aaagccattt
     4981 aaaccactcc actgctgtgc ccgtctttcc ttaccgggcg gaccgggcca agtttgatga
     5041 cattttccgg atcaaagtcc gtgagtttga gccagtcctc ggtcacatcc gtcggcggga
     5101 acaaatcgtt ttcctcgtac tcgcgatgga gcgcgtggat gaggtcttcc cgggtcaggt
     5161 tggtttcctg gcctgtctgg atggaacgtt ttatcgcgaa actcttcgcg cgttcgacca
     5221 cggctgcgat gatcgcgcca ctggcaagat cgccgcgata caaaaagtcc ttcttgccgc
     5281 tgcgaaagac gatttccaaa aactggttcg tcggcgaatg ggcaaaatgc tcttcagtga
     5341 cggccttggc cagatcgggc cgcggttcgc ccagcggaag gctttcacgc aggtaaatct
     5401 cataaatggc ctgcgcccct gccttgtcgg gacggttcac cttaattttc cggtcaatgc
     5461 gtcccggccg caaaatagcc ggatcgatca agtccgcccg attggaggcg aggatcacca
     5521 ccacgttttg cagtggttcc aagccatcca tctccgtgca gaacatcggc accagcgtgc
     5581 tcaagatgct gctgtaacgg cccgaacggc gcgtgcccag gatggattcg gcctcatcaa
     5641 tgaacaaaaa tgccagagcg ccatcgcggg cccgttcgcg gcattggatg aacaagtcgc
     5701 gcacctgccg ttcggattca cccacccaca tgttgaggat ttccggaccc ttgacgtgca
     5761 ggaagaattc agggtggtca acacctgtct gcgccttgat ttgctggcgc agattatagg
     5821 cagtcgcctt tccgagcaaa gtcttgccgc aacctggagg tccatgcagc aaaaagcctt
     5881 taggaaccgt atgctcaaaa cgtttgaaca gatcgcgatg caagaacggc agttcaatcg
     5941 tgtcacgaat cgcttgcacg gcttcggact gaccgccaat ggccgaccat ggcagttctg
     6001 tgaccgtttc cagcgagcgt tcgatgcgtt tgcccatgcc gatcacttcc agcgcgacgc
     6061 gctggttcac gtccaagcgg acttccatgc cgggcttcaa tttttccttc accagcaaag
     6121 cggaacggag cagcaccaaa gacatcaggc ccgcttcctg cccgatgcgg aggcggccat
     6181 cagaaagtaa ttcatcaatg cggacgattg gcccgttcct gtcgaagccc aatccctgca
     6241 ccacggcgaa ggcttcgttg cagagaacgc gctggcccat ctgcagactg ttgagcggga
     6301 tttttgggtc cacgcggcag acgtaatcgg tgccgcccac gcacacgtgt gccttgtcgt
     6361 tttccacagc ggttaaaaaa gtgccgacgc ggaacgcggg tgaacgaagt ttttcgacaa
     6421 tttcatccaa cttttcgagg gcttgcctcg cctggtcgtt catctcttcg atcgcggcga
     6481 tgcggtcgcg cagatagatg aggtccatga gcgatgaatg ttgcggcgga agttttccgg
     6541 cgattagatc cacgagttga agggctgaga gagcctccat ttcttcgtgc gagagaattt
     6601 gtccaagtgg ggacgggcct ccttcagcct gcgtctctgg cttgggcgat ggcgttgttt
     6661 ttttctttga ttcacccatg cctccacggt agccctcttc gccagaaatg caaatacaag
     6721 atggcgattt ggaaaacaat tttgaaatgt aattttgctc tgacgcaaaa ttatttttgc
     6781 tcacgcaaat tcttcaatgc ccgcccgaaa ttagcaaaaa gattttattg cggcaaaaaa
     6841 ctccaagcat aagaactggg aactttgcag aaaccagaat gagaaaaaac gcgtgttctt
     6901 cacgcgccga atcagctaaa acacgcgggc gccaggcaat gtccttttgc gcgattccta
     6961 atcttgttct tccccgaatt cttccgcgcc gccttggaga agactgaacc cgaggatgcc
     7021 aagcgccaca cccagtccaa agattatggc catgctggga taagggttgc ggcggatggt
     7081 ttcgtcggtg gctttcgcgc ccgccactgc ttttatgcgt aattgtcctg cgccgccttt
     7141 gagcaattcc tgaccgtcat caaccacgac tttcaagtcc tgcattaatt tttccaacgt
     7201 catgccgtgc ccatttcctt tgcgagtttc tgtttccata cgagtgtttc cttccgttca
     7261 agaatactca caggctcttt gcccacctat ggggtcttac ccgatggaag actgcgattt
     7321 gggatttcaa attgaaatct gaaatttgag atttgaaatt tcgaattaag aaaaaaaggc
     7381 gcgccgcgct tttttcaatt cgccccgtgc aacacatctg tttttccgcg cgattccagg
     7441 cgattgacgg ctggttcacc aatcggcgaa ttcaccagca aaacggattt gggttcgtca
     7501 cgttcgttgc gcaacaaagt gcagcggctg tgcacgagaa tctcatggtt cgcccgatcc
     7561 acctgatgca agtccccctg ccattgtccg ttcttgagaa tggcttgatt ggccacctgg
     7621 aattccgagg tgtcgtggta aagcaattcc gtgacattgc gtcccaacgc ttcctcgcac
     7681 gaccatccat acaagcgcgt ggccgcttca ttccaatatt gaatgcgtcc atccagatcg
     7741 cgcaccaaaa tcgcttccgg caaaaggtca agcaactgcg accgctcgcg aaactggtcc
     7801 tcggcttgtt tgcgtttcgt gatgtcgcgg cataggcaga gcacgaggtg acgcccgtaa
     7861 agttccaatg tgcttgcgcg gacctgcacc ggaaacgtcc tgccatcctt gcagatgatt
     7921 tcactttcga cttcggcccg ggtattatgg gcgagaacat ccgccagcac cttgcgataa
     7981 gcttcaaact tcccgtgcac atgaaagcac tcgtgtttgg ccccgatgat ttcggaacgg
     8041 gcgcgtccca aaaacgactc ggcgcgttgg tttgcatcca gcacgcgacc ggtgggcaga
     8101 tcgacgagaa aagccgcctc gctcatgctc tcgaatacct ggcggtattt gtgttcggac
     8161 tcgcgcatcg cttcttccgc gcgcctgctt tccgcgtggt cttcgacctc tttcaatgcc
     8221 cgtcggaccg cgggcaggag gcgcgacagc cggttcttga aaacataatc ggcggcgcca
     8281 tttttcaggc tctccaccgc cacgtcttcg tcaatcgttc cggaaacaaa aatgaacggc
     8341 gtgtcggcat gatgctgccg cgccagcgcc agagcttcga tgccgttgaa agaggggagt
     8401 gtgtaatcgg aaagaatcaa atcgaacagc ccttcgttca atgccgtcgt gaacgctttc
     8461 gccgactcga cgcgcaccca atcccgcacc aacccttcgt gagtcaggat tcgccggagc
     8521 aattctccgt cgaacgggtc gtcctccaaa tgtaaaatac gcaatgcaca attcttcatt
     8581 accattcaat aatcactgtt ggcgcgaccg gataacgtcc gcattccgcg ccgtaaattg
     8641 tcaatccacc ctgggccagc gcgtcttccg ggaccacgca ccgcaaccgc aggctgtcat
     8701 ccatcacatg caaggcaatt tgccgcacgg aatccgcttc cgcaaatgca tgcaccaggt
     8761 aaccgtaagc gcctaatccg ccgcgaagat agctaaggga gccccgcgca tcatggggat
     8821 gattgcggat ccacgcgcca taaacctgga tgccgttgag aaacatctgc aacgtcgtag
     8881 gaaaaatatc ggcgtcagtt tgcggcgtgt caatccggtg tgacgatgcc tcgcacaaca
     8941 cgcggatgcg gcgcgccttt gtgacgtccg ccccgcccag cggcaatacc cattcaaaaa
     9001 agccgtgccc cattccgaag cacgaatcct ccgcgccagc gcgcgcgcgt tcgctcatcc
     9061 ccccgccgct ccattcgccc gcggcccaat cgccgggcgt tccgcgcagc accattgcgc
     9121 ggggtccttc ctcgcggtgc ggtggataaa cttctgaaac gaaatattgc agaaaattgc
     9181 tcgccaccac cgttccttct tccgtgcagg cctcaagcca taacgtgcac aacatcggtt
     9241 cggaaggcat tcgcaattcc agtgtatgcg ccggcacgac gcggcgatgc ggaaattcaa
     9301 tcggcacaca accgcaagtc acgctttggt ggagatgacc gcgcatgtca atcccatcca
     9361 ttttccaacg caaggaaatg ttgcgcgtct ttcgcgagga aaaatgggac gacgccaccg
     9421 gcaggcagac agtttccccc ggtcttactc gtgaaatcgg cggcgcatca atcggtatcg
     9481 tatcactttc attgatcatg gccgggtcgt aaccgaattc cttcggcgtc cggtcgtaat
     9541 tcaagaaacc gttatgctcc cattccacgt cgtgcaactc ggtgaaaatg tacgcggaaa
     9601 tctgcccgta tcgccgcagt tcgttcgtga ggaatttgaa gctccaactg atgtcgcggt
     9661 cgccgtcaag cgcgcccacg ccgccgtatt cgctgttgat cagcggctgc cccttgtgcg
     9721 caaaaccggg aacgtaattg aaatttgagc cgggaaaagt ggatttcacc accttggcga
     9781 tgtgctcgcg cgctctttcg tattcatcaa tgtaaaaatg ccacgagttg atgtcggtgt
     9841 cgccgtgcgc gaaataatcc aggtgctccc aatgcaccac gctcatgtcc tcgatcaaac
     9901 gcgtgggatc caatttcttc gccaattccc acatcatttg cacccatgcg tgcgaactga
     9961 ggttcgccat cttggcccgc ggggccttga cgggagctgt catggtcgcc gttacaactc
    10021 cgccgccgtt tcctccattg ctgcgggccg agactgcgcc gggcgctttc tggccgcgcc
    10081 gccggtcttt gggattaagc gatttgatga tgtcggcgaa ttcagcctgt ccgccgaatc
    10141 cccacgtctc gttgaaaatg caccacgcaa aaatggaagg atgattgaaa tcgcgctcga
    10201 tggcttcgcg catcattatc tcgaagcgtc gcctgcccaa cggcgtgtcg ccgccttcgc
    10261 caaaattcgg gatgtccgcc atcaatagca tccccatgtt gtcggcgtga tacagcagca
    10321 acgggtcgtc gatcttgatg tggatgcgca gaaaattgaa cccgactttc ttcgcgtaag
    10381 cgatgtcgtt cttgaaagtc gccacatcgc ctgccgtgta aacgccctcc ggataatacg
    10441 actgatgcag cgccccgcgc aaatatcgcg gctgtccgtt caaccgcagc agcaccggcg
    10501 actccggcgg ttctcccggc gcaaaatcaa tctttcgcat cccgaaataa gtccggacag
    10561 tgtcgagcgt ctgtcccgcg cgttgcagtc gcaaaatcac cttgtaaaga ttcggctcgt
    10621 tcgggttcca gagcagcaac gggttcacct tcaccgcttc ctttactttt ccatcgcgga
    10681 cggggaattc agctttttgc ggcgaatggc cgggcggcaa aatttccgcc gtcacaacgc
    10741 aatcatccgc ggcattttcg caatcaattt caaaccgcgc cgtacctgca tcaatgtccg
    10801 tgtaaatccg aaagagccgc agaaacgaag cgtctcgcgg ctcgatataa accgtctgcc
    10861 aaatcccgct gatcgtagtg taccagcgcc attgcttgcc aacgggctgt tcgtggttgt
    10921 ccatcgggtc ttccacgcgc aagacaatca ccgcctcccg ctttccatcg gcactcgttt
    10981 ccaacgcatc ggtcaggtca aattcaaacg gcgtgtaacc accttcgtga tggccaaggt
    11041 gctgtccgtt gcaccagcaa tcggtgaaaa aatctgccgc gccaatcgtc aatatcaccc
    11101 gcttgccctc ccattcctcg ccctccggta tctccaccat gcgccgatac catcccactt
    11161 cataacgctg cgccgagcga tggttgcttc ggtccacttc cagcggattc aggaaaacgc
    11221 gcgtcgcata ataattttca tttcccgcgg cgtcgccttc gccccatgcg gccagggatt
    11281 cccagcaaaa cggcacgaca atctgttccc gccattcggg ctgctcgact tcatgccagc
    11341 cttgctcgag gccgcgacgg taccggtcga accggaattg ccacgggccg ttcaagttca
    11401 gccaatcgat gccttccaaa gttccgcgtt gacgatcagg ccggggatat tccggacggg
    11461 ggtgcgctag taaaatcgac ataaaagcaa tctatctata aagtgacaca aacgcttcgt
    11521 tcctgctcaa acgcaaaatc tcccgttcgc agtcccatgg atgcgcggtc agtcagaaag
    11581 tcgcacggtt gcgcatcccg ttccaaggga ggtgaattac ggagttgctt tggagaactt
    11641 ttcggggcag gcgagcttaa ttcgccgcgg tatttggggg ctgttgcttt cgtttctcaa
    11701 ggacctcgcg gattttgttc cccaattggc gggaaaatat cgccgccgct ccccggcgaa
    11761 gatgcaaagt atccaaaaaa tattcatcgg gataatccag ctttccccag tcaatcacga
    11821 ccacgttggg gatcgcggcc agttccttct tataggcctc aaacttgtcc tcattctcaa
    11881 aattcgcgta ataagagggg tggtaaggtg aaaccaccag gaagaacaga cgctggggat
    11941 ggcccttgat atgcgcaatg aaccttcggt tctggtcctc gtccggataa tacccatttt
    12001 ggtatgccaa tcgctcacga atgtattggt caagttgcgc ccggttaaat ggaggggttc
    12061 gcaccagttc gccaaaaccc tggctcattt ttgacacgtg gaaacagcca ctgaggtaag
    12121 tggacaaaat aaaatcgtag taaccgaagt aacggatccc tggaaaatga tagcgccagg
    12181 acatcgcgtt gaattttttc aacaacggct gaaaccgggg atccgaggcg aatggaatga
    12241 actggctttt ctcgcccaga atgcccgtgt cccgatgttg caattcgatg atgatgggag
    12301 tcgtcctggg cttggccagc tcgatttgaa ggaaggtatc caccacctcg taagaggcgc
    12361 cgttaaaacc atagttgtac gctttgatgc ccatgtcgtt gggggagtaa ttaccatgcg
    12421 ccttcgatgg gccgaagatt ggaatgtctt cgctgttgtc ttcgcaaagg tgtttgatgc
    12481 gcgcgccatc ctgtgacgga tcacggaaaa tgatgatttc cgtgaagacc ctatccacag
    12541 caatgcaaat gccagccaaa caagcgagcg ccagaatcca tgttttgcat ctcataaagg
    12601 tcaaaatgca gcgtaaataa aatgcgccga tcccagtgag ccaagcagca atacgagcgt
    12661 tccgcataac accgcgaacc agggtgtcca acgatccgaa gtgcggcgac ggagagttgc
    12721 gaccgcgaac caggcggcaa gggccatgca attccacacg aaattataag gcctggcgcc
    12781 cagcaagtca tttagactgc cactcatgcg caggtggaac aatcggctga aaaccgtccc
    12841 cgcctgcgaa agattactgc ttcgaaacaa cacctggctg agcgcaaaaa atcccagagt
    12901 ataaaggctg gcaaccatga tgacgatgcg ttttggcgcg cgcttcagcc attttgtccg
    12961 ccatggcttg gtaagaaact ccgcgcttaa tgccacacct tgagcaatcc cgaacaggag
    13021 gaatggccat gttgcggcat gccagatgcc gcagatggca aacgtgatga tcaacgccaa
    13081 agctatcccg accttgccca aggcgcgaat ccgtatcacg agcggcatgt aaagatagtc
    13141 gcggagccaa aaggaaaggc tcatgtgcca gcgcctccac aactcagtca tcgaagtgct
    13201 caagtatgga cggtcaaaat tgggctcgag gcggattccc agtaatcttg cgctgccgat
    13261 cgcaatatcc acgtagccgg aaaaatcggc atagagttgc aaagcgctga aacaaacggt
    13321 caggaaagcc gatgtcgtgt tggcggtttc cgggtggtcg aacacgccgc tgacatagag
    13381 cgaaagccgg ttggcaacga cgtacttctt gaaaagcccg taggcgatca atcgaatccc
    13441 ggcgagcctg tcctcggctg tcgggcaaca actcagttgc ggcaacaggc ctggcgcgcg
    13501 cggtatcggt cccgaagtga tttggggaaa gaacaggccg aatacagcga accgtgaaaa
    13561 acctttctcc atgcgccatg actgccgata gacatctgtc acgcaagaca gcatctggaa
    13621 ggtgaaataa gagagtccca gcggcacggc cagtcgcgcg actttgccac cttcgccggg
    13681 ccagccgaat tttgccgtca ccccgctcag caattccgat gcgtatttga aactgatcag
    13741 caccgccagg tttgccacga ccgccccggc gaaaaaccag tcgcgccggc gcgggtcctc
    13801 ggttgcggac aaacctcgga gcagccagta attaatggcg caaagcatca gcaacagaaa
    13861 aagataactc acactcgaaa acgcatagaa agcgaggctt gcgcaaagca gcaaggcgat
    13921 ccgtcccctc tcaggcagat acgcctgaag gattgcgacc caggccagaa agagaaaaaa
    13981 aatggcgcta tcaagtccca tggtcagtcg cgcatcaggc ggattccatc tgtcggattg
    14041 attatggaat ccatgaaact caagattgtt tgcggcggat cagcgccgca agatcgccca
    14101 ctttttgaag ttgctgaatt tccccggcgg tgaatttgac ccggaacttc ttttccacag
    14161 cgacgacgat aaggacatgc gttatggaat cccacccgtc cacgtctttt gcggttgttt
    14221 tttcctcaag cacaagcgag tcgttgtcga gcacatcttt gaaaatgccg gtcaaatcgt
    14281 tccataaggt gtcgttcata agatcaagga ttacggctct ccatggtttc ttcgggaatg
    14341 atgcaggtcg aacgctcaag atagccgtcc gtttcaagcg tccaacgatt gtctgcttcg
    14401 acggcaaaac ccatcttcaa atagtgattt tcgaccatct tatttttgag ggttggcagg
    14461 tattcgccaa tgatgcgttt gatgccgcgc tcccgggcct tcgccatgat ggcgttcaag
    14521 acaaactcct ccacaccccg gcccaaaaca cggcaactca tcaaccaggt gtccacaaaa
    14581 aattcgttcc ccaagatttc caggatgacc actgaaatca gcccattgtc gccgaaacgg
    14641 tccttcaagc tgaaggtcag cgtcacgtgt ttgggcgagg cggccagcgc cgcgatttcc
    14701 ttttccgcat aacgcacagt gcggagattg aactggttcg agcgttgcgt gagttgcgcc
    14761 acgcggggaa atgtgaacgg aagaaagggc tgcgccagca tgcgcatctc aaggctgcgc
    14821 agaaaatccg ggagattgat aaatttctcc ctctccacat gccgcaaggc ttctgtctgg
    14881 tattcggccg tgcgttgcgc gtcttgtgcc gtataactcg cagtttcgaa cgggttcagc
    14941 gacgtcaaat aagccacata ctcagaaggg tccgccggca attcgggcac ctgcattgtg
    15001 ggaaaactct cgcgcaccaa ttgccgttcg gcggggttgt catccaggaa catcatcgcc
    15061 ccataatcaa catcaatgat ttttttgatg caccggatgt tggcggcctt gtcatcccaa
    15121 ttggccatga acacggcgat gtcctccagt cgcaacacca tgtctggatg tttcaaaaac
    15181 ggctcgcggg cattggcctc atcattcttg ctgcacacgg ccagcacgat gccccggtcg
    15241 cgcagttgtt tgatccatga ttgaaacgcg ctgaaagccg gcccaaggcc aagatcgcca
    15301 atttggatcc gctccagccc gtcgtcgccg atcacgccgc cccacaaagt gttgtccagg
    15361 tccagtatca cgcatttgac aaaggagcct ttgcgcgccg cgagcatctt gaccacgttc
    15421 cgcgccacga gcggcagaac tcccggcgca agcgcgaact tcgatgtgta ataaagcttt
    15481 ggatcatgca acgcgccgcg gcccaactcc gattgcaaag ccgacaaatc gcatacgtgg
    15541 aagtcatccg cttcgcccgc caatcgcgcc agctccgcat tgcaaaagcg gatctgattt
    15601 tgtaaagacc aatggacttt gttcccataa tttccgaaca cgccgtcgcc ggggtcggcc
    15661 aggttgaaga acagcgtttg gcaaccggaa tgagacaaag acttgtgcaa ggccctgacg
    15721 cgtcctgaaa agttgacggc aaaatcgcct cgttcggcaa tggacaaagt cccaaacttg
    15781 tcccgtgtcc gctccgccgc aacgtaaaca atcaccgtct caggccgaaa gctgtgcaag
    15841 gccgatgccg ggtcaaaaac cagcggttca atttgatcaa agtcaccttc aaaaaggtcc
    15901 agctcaaaac ccaggtgccg cgcatagccg cgtagcgcgc cggcgagcag ttgcgacgaa
    15961 gtgtcggcaa ggagcgccgc gcgaagaatc gccgcgtcgt ccgcgcgggg tcgccgggca
    16021 agcttcaaca aggtcgttaa ttccggcaaa tctgtcatga aatcttgagc ttatgacaat
    16081 acgctctcgc ttgacaagca ccgactcccg cgttttggca atgcaacatc tcttgtcttc
    16141 cgaagccatt ggaaatcaac cttgggccgg tgcgtctctt ccctgaaaac cacttgcgcg
    16201 gcgctctttt ttaagctacc cacaggggcg tgtcaaaact cagacactgt tttttctatc
    16261 ttatttttgc gggcattctt tgcccgttgg tggccgtcgc ccaaattgat ccggccaaac
    16321 gcgagcttgt ccagatcggc tacaaccaat cccttatcgg ccaggccccg ctctccgcct
    16381 acgcatttta ctattacaac aagcccgatt tcctcaatga atccaacctc accctgcgcc
    16441 tcgccgtcgc gcccgtctat gtggattccg aactcggcat cagccatgcc ctcggtcctg
    16501 acaccgatgt cggcatcgga cttgccggcg gcgggtttgc cgacagctac tacgaagttc
    16561 gtcagggaaa atatttaaag gcggaatcct tcgatggcga tggcgtcacc ggttcaggca
    16621 gcatctacca cctcttcaac cccggccagc gcatcccgct caacggcctc ttccgcgtcg
    16681 aaggccatta cgccgactac attcgcgacg acaccgcgcc caatttcgtc ctccccaagg
    16741 acatgactga tttcaacatc cgcaccggct tccgtttcgg cggcaaggaa cccgtcatgg
    16801 ttcctgacat ggccatggaa ctctccctct ggtatgaagg ccaattccgc ctcaactccg
    16861 gcgactacgg cttcaacaac gaccgccacg tcaattccat ttcgcacctt ttctggggcc
    16921 gcgcactcct cgcctacaca ctgcccgaat cgaaacaaaa cttcctcgtc agcgtcactg
    16981 cgggcgacag catccaagcc gaccgtttca gcgcctatcg catcggcggc ttcctgccgc
    17041 tcgccgcgga atttcccttg agcctgcccg gatattttta ccaggaactc agcgccacac
    17101 gcttcgccct cttcaacgtc aactacaccg tccccatcga tgccgagaaa cgcttcgcct
    17161 tcaccgccat cgccagcacc gcaatcatgg aatacctgcc cggccttgag caacccggcc
    17221 attggaactc cggcgtcggc ggcggactca gctaccattc ccactccgac gcatggcgcg
    17281 tgcttgtgga ctacggctac ggcttcaacg ccatccgcga caacgggcgc ggcgggcaaa
    17341 ccatcggcat actattgcaa atcaaccttg agcgcaccca cccgcaatac gtcgtgcccg
    17401 gcagcggcag cggcatcctt cgcggcctcg gcgacttcac ccacagcatc ttctgaccag
    17461 ccagcgcgaa agaccgtaaa ctcccctaag ctcccctaca ctccccgaac gagaacgtga
    17521 aagttgttaa aacctttcat aatcactaac tacctcgtga acgccaacga taaagtaacc
    17581 aagaactaag gttttttctc actttttgta gctgaaaacg taagttttca gacggagcgc
    17641 attcatccgt gtcatctgtg gctcagtccc tcaaccattc gcaggccgac gtgagggggc
    17701 tcgcctcaga tttgaaactt caaattccaa tttgaaatcc atacagagac tcctcacgtc
    17761 gtctcctaca ttatgggttc gcggcattct tcagcttccc cggcattcat ccgtgcaatc
    17821 cgtgtcatct gtggctaaac cttaaacctt gaacctggct tggccgctct caacaatttc
    17881 ctcacgcacc gctccggatg ccgcaactca tgttcccaga gacgcaccac acgccagccc
    17941 tgacgccgca acgcccggtc caccagccgg tcacgcgcgc ggttg
//
LOCUS       DASZMM010000561         6119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4394837, whole genome shotgun sequence.
ACCESSION   DASZMM010000561 DASZMM010000000
VERSION     DASZMM010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4394837"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VG754_13465"
     CDS             <1..846
                     /locus_tag="VG754_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HWF20272.1"
                     /translation="RTRASSIRQDGNHWFINAHHAITGPLLISAENLFLCAGAVQTPA
                     LLRRSGITQNIGNSLRLHPTVKVVAKFPEPINSAQMGVPVHQVKEFAPRFSFGCSIST
                     PPYLSLALLDHPEQARAVTRDWPQMATYYSMITGQGHGTIRTVPGCRDPLVRYHLTES
                     DRRDLADGLRKLCRALFESGATALYPSITHSPFFRDRNELSALPNALPNGLTSLMTIH
                     LFSSCPMGEDQHKCATNSYGRVHGFKNLYVSDASLLCTAPGVNPQGSVMAFARRNVMK
                     FLRAI"
     gene            859..1923
                     /locus_tag="VG754_13470"
     CDS             859..1923
                     /locus_tag="VG754_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF20273.1"
                     /translation="MDEFKTNKVIVTGALGWLGISLVQSLVKGLADHEALKQPRNDLR
                     VRCLILPGQDAAPLRKISDRVEVMTGDIRNAGDCARLCEGFEGGILFHTAGIIHPGKV
                     AEFYQINVEGTKQLLDAAISAGIRRAIIVSSNSPCGCNPHNDHLFDENSPYHPYMNYG
                     RSKMQMELAVKEHQKPGKIETVIVRPPWFYGPNQPPRQTLFFQMVRDGKAPIVGSGNN
                     LRSMGYIDNLCQGLMLAALVEKANGQVYWIADKRPYSMNEVIYTIERLLETEFGQKCA
                     HKRMRLPGFASEIALVADKTLQALGLYHQKIHVLSEMNKTIACSVARAEKELGYRPAV
                     SLEEGMRRSMAWCVQQGIKF"
     gene            1927..2958
                     /locus_tag="VG754_13475"
     CDS             1927..2958
                     /locus_tag="VG754_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF20274.1"
                     /translation="MNGALITGGSGYFGSLLRDRLRAHGKPVRIFDLSDADDRPKDVS
                     FVAGDIRNEAQVMEACAGCGVVYHCVAQVPLAKDKHLFDSVNIQGTENLLKAALNAGV
                     RKVIYVSSSAVFGVPKSNPVTEATTPTPAEAYGRAKLEGETLCAKYAQQGLDVTIIRP
                     RTIMGHGRLGIFQILFEWIRTGYNVPVLGSGENIYQFVHADDLAEACILAAARPGTAT
                     YNCGAERFGSMRAVLEYLCAHAKTGSKVKSVPMAPAIAFMNFTSALGLSPLGPYHAMM
                     YGRSLYFDITKAKTELGWQPRFSSNEMFVQSYEWYLTNREQILRSHGASHHRSAVKQG
                     VLSLVKHLL"
     gene            complement(2983..3810)
                     /locus_tag="VG754_13480"
     CDS             complement(2983..3810)
                     /locus_tag="VG754_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF20275.1"
                     /translation="MNSDLGGKVVLVTGASGGIGSAIARKFAAEGARLILHCHANRAK
                     AEALATELKPAESLVLRANLTREPDMKRMFAAALKKFGRVDVLIANAGSWETRDVPLH
                     QMPLKQWRNTLDAVLTSAFLSLREFFRIVAKQKRGNAVLIASTAGVFGEAGHADYAAA
                     KSAMACGLTRTLKNEIARIAPHTADYCGGRVNCICPGWTIVPRNAAKLGDVATVRKVT
                     ATMALPQIGHPDDMANAAVFLASDTLARHITGQTLVIAGGMEGRLLWKPEEIDAAIV"
     gene            3938..4738
                     /locus_tag="VG754_13485"
     CDS             3938..4738
                     /locus_tag="VG754_13485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWF20276.1"
                     /translation="MKRFLLLTTAGLLIAASASAQDWAKAKLEKSPRHGEWVKLHHDN
                     RDVESFITYPEVKDKATAVVVIHEIFGLSDWARNVTDELAAAGYIAIAPDLLSGKGPN
                     GGGTAALGGDDGARKVIGSLPPNQITADLNAAVDYVSKLPSCNGKVVVMGFCWGGGQA
                     FRFATNNKDIKAAFSFYGPIPDESTFSRIQCPVYGFYAEKDARITSTVPSAAEAMQKA
                     GKTYETVVYEGAGHGFMRAGEAPDATEANKKAHDQAWQRAKELLSKIN"
     gene            complement(4757..>6119)
                     /locus_tag="VG754_13490"
     CDS             complement(4757..>6119)
                     /locus_tag="VG754_13490"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HWF20277.1"
                     /translation="KHETAAGQSLAELLEQNAFDRAQHEEIRADLKNGRIGLAQNRLP
                     ASTKIEDVHPDDVLDATGGLPADYATTGKAAISNGEVAVVTLAGGVGSRWTQGAGVVK
                     ALHPFCKLAGRHRTFLEVHLAKSRRVNREFGATLPHVVTTSYLTHGTIESFLASQKNY
                     GYSGPLLLSPGKAIGLRMVPMVRDLRFLWEEMPQQLLDEQAQKVRESLHAALIGWAQA
                     GEGNDYTDNLPAQCLHPVGHWYEISNLLRNGTLARLVRERPKLKYLLLHNIDTLGVDV
                     DPALLGLHIRQGACLSFEVITRRIEDRGGGLARVNGRVRILEGLTMPREEDEFALSYY
                     NTLSTWIDIEKLLALFGLTPGELEDSEKVSAAIRALAVRMPTYITLKEVKKRWGQGQE
                     DIFPVAQFEKLWGDMSALAEADCRYVVVPRLRGQQLKEQAQLDGWLRDGSAAHVESLC
                     EWE"
BASE COUNT         1320 a         1784 c         1700 g         1315 t
ORIGIN      
        1 cgcacgcggg caagcagcat tcggcaggat ggcaaccatt ggttcattaa tgcgcatcat
       61 gcaatcaccg gacctttgct catctccgcc gaaaatttat tcctttgcgc gggcgcggtg
      121 caaacacccg cattgttgcg acgaagcggc atcacgcaaa acattggcaa ctcgctgcgc
      181 ctgcatccga cggtgaaggt ggtcgccaaa tttcccgaac cgatcaattc cgcccaaatg
      241 ggcgtgccgg tgcatcaggt gaaggaattt gcgccgcgct tcagtttcgg ctgttccatc
      301 agcacgccgc cctatttgtc gttggcgctg ctggaccatc ctgaacaagc gcgcgcggtg
      361 acacgggatt ggccgcagat ggcgacctac tactcgatga tcaccggcca gggccatggc
      421 accattcgaa ctgtgccggg atgccgcgac ccgcttgtcc ggtatcacct cactgaatcc
      481 gaccggcgtg atttggcgga cggtttgagg aaattgtgcc gtgcgctctt cgaaagcggc
      541 gcgacggcgc tgtatccgtc catcacgcat tcgcctttct ttcgcgatcg gaacgaattg
      601 agcgctttgc ccaacgcgct gccgaacggc ctcacgagcc tgatgaccat ccatcttttt
      661 tcgtcgtgcc cgatgggtga agaccaacac aaatgtgcca cgaactctta tggccgcgtg
      721 catggtttta aaaatcttta cgtcagcgat gccagtctgc tatgcaccgc gccgggcgtg
      781 aacccgcaag ggtccgtgat ggcctttgcg cggcgcaatg tgatgaaatt tttgcgcgca
      841 atctgaaaac aaattctcat ggacgaattt aaaacgaaca aggtcattgt caccggcgcg
      901 ctcggctggc tgggaatcag cctggtgcaa tcgctagtga agggcttggc ggaccacgaa
      961 gcgttgaaac aaccgcgcaa tgatttgcgc gtccgttgcc tcatcctgcc cggtcaggac
     1021 gccgcgccgt tgcgaaaaat ttcggatcgc gtcgaagtca tgacgggaga cattcgcaac
     1081 gcgggtgatt gcgcgcggct ctgcgaaggt ttcgaaggcg gaattttgtt tcataccgcc
     1141 ggcatcattc atcccggaaa ggtcgccgag ttttatcaaa tcaatgtgga gggaacgaag
     1201 caattgctgg atgcggcgat cagcgcgggc atccgtcgcg caattatcgt ctcctcgaat
     1261 tcgccgtgcg gttgcaatcc gcacaacgac catctcttcg acgaaaattc tccgtatcat
     1321 ccctacatga attatggccg ttccaagatg cagatggaac ttgccgtgaa ggagcaccag
     1381 aaaccgggaa aaatcgagac ggtgattgtc cgaccgccct ggttttacgg gccgaaccaa
     1441 ccgccgcgcc agacgttgtt ttttcaaatg gttcgcgacg gcaaggcgcc catcgtcggc
     1501 agcggcaaca atctgcgttc catgggttac atagataatc tatgccaggg cctgatgctc
     1561 gccgcgctgg tggaaaaagc gaacggccaa gtttattgga tcgccgacaa gcggccctat
     1621 tcgatgaacg aggtgattta caccattgag cgtttgttgg aaaccgagtt cggccaaaaa
     1681 tgcgcgcaca aacggatgcg cctgcccggt ttcgccagcg agatcgcgct tgttgccgac
     1741 aaaacacttc aggcgctcgg tctttatcat caaaaaatcc acgtcctttc agaaatgaac
     1801 aagaccatcg cctgctccgt tgcccgcgct gaaaaagaac tcggctatcg ccctgcggtt
     1861 tcgctggagg aagggatgcg ccgctccatg gcgtggtgcg tgcaacaggg tatcaaattt
     1921 taaatcatga acggcgcatt gatcactggt ggctcgggtt attttggttc gctgctgcgc
     1981 gaccggttgc gcgcgcacgg aaagccagtg cgaatttttg atttgtcgga tgccgatgac
     2041 cggccaaagg atgtgagttt tgtcgccggt gacatccgca acgaagcgca ggtcatggaa
     2101 gcgtgcgcgg gatgcggcgt tgtttatcat tgtgtggcgc aagtgccgct ggccaaggac
     2161 aagcaccttt tcgattcggt caatatccaa ggcacggaaa accttctgaa ggccgctttg
     2221 aacgcgggcg tgcgaaaagt catttatgtt tcttcaagcg ccgtctttgg cgtgcccaaa
     2281 tcaaatcctg tcacggaagc caccacgccg actcccgccg aagcttatgg ccgcgcgaag
     2341 ttggagggtg aaactttatg cgcgaaatat gcgcaacaag gcttggatgt gaccatcatt
     2401 cggccgcgca ccatcatggg gcacgggcgt ctcggcattt tccagatcct tttcgaatgg
     2461 atacgcaccg gttacaacgt gccggtcctt ggcagcgggg aaaatattta tcaattcgtt
     2521 cacgcggacg atttggccga agcctgcatc cttgccgcgg cgcgccccgg cacggcgact
     2581 tacaattgcg gcgcggaacg gttcggttcc atgcgcgccg tgttggaata tttgtgcgcg
     2641 cacgccaaaa ccggctcgaa ggtgaaaagc gtcccgatgg ccccggccat cgcgttcatg
     2701 aacttcacca gcgcgttggg attgtcgcca ttgggaccgt atcacgccat gatgtacggc
     2761 cgctcccttt atttcgacat caccaaggcc aagaccgaac tcggatggca gccgcgtttt
     2821 tctagcaacg aaatgttcgt gcaaagttac gagtggtatc tgacaaatcg agagcagatt
     2881 ctccgctcgc acggcgcgtc gcaccatcgt tcagccgtaa agcaaggcgt gctgtcgctg
     2941 gtaaagcatt tgctctgagc cattgcgcaa aacatgcggc tcttatacga tggccgcgtc
     3001 aatctcctcc ggcttccata gcaggcgacc ttccatgcct ccggcgatga ccaaagtttg
     3061 gccagtgata tgccgcgcca aggtgtctga ggcgagaaag accgctgcgt tcgccatgtc
     3121 atcaggatgg ccgatttgcg gcagcgccat ggtcgcggtg actttgcgga ccgtcgcgac
     3181 atcgccgagc ttggcggcat tcctcggaac aatggtccaa ccgggacaaa tgcaattgac
     3241 gcgtccaccg caataatcgg cagtgtgcgg cgcgatgcgg gcgatttcgt tttttaacgt
     3301 gcgcgtcaaa ccgcaggcca tcgccgattt ggcggctgcg taatccgcat ggcccgcctc
     3361 gccgaacact ccggcagtcg aggcgatgag caccgcgttc ccgcgcttct gtttcgcgac
     3421 gatgcggaaa aattcgcgca agctcaagaa tgcggaagtc aacaccgcgt ccaatgtgtt
     3481 gcgccattgt ttaaggggca tctggtgcaa cggcacgtcg cgcgtctccc acgaaccggc
     3541 gttggcgata agcacatcca cgcgtccgaa ttttttcaag gccgcggcga acattcgttt
     3601 catgtcaggt tcgcgtgtca ggttggcgcg caaaacaagc gactcggcag gtttcaattc
     3661 tgttgccaac gcttccgctt tcgcccgatt cgcatggcaa tgcaaaatca gccgcgctcc
     3721 ttccgccgcg aattttcgcg cgatcgctga accgatgccg cccgatgcgc ctgtgaccaa
     3781 aaccactttc ccgccaagat cagaattcat gcgattcttt tagcgaataa caacgcacgg
     3841 ccaaagtcta aagtccacgc cctgcccttg cggattcaat cgcgcggtgt tatagaatca
     3901 tccaccgaac cgatcatcac tcgaacaaag gaattgcatg aaacgatttt tgcttctcac
     3961 gactgccggg ttgttgattg cggcatcggc ttccgcgcag gattgggcca aggccaagct
     4021 tgaaaaatcg ccgcgccacg gcgaatgggt caagttgcat cacgacaatc gcgatgtcga
     4081 gtcgttcatc acctatcccg aagtaaagga caaggcgacc gccgtagtgg tcatccacga
     4141 gattttcggc ctttcggatt gggcgcgcaa cgtcaccgac gaactggccg cagcaggtta
     4201 catcgcgatt gcgccggacc tgctttcagg caaagggccg aatggcggcg gcaccgcggc
     4261 attgggaggc gatgacggcg cgcggaaagt aattggttcc ttgccgccaa accaaatcac
     4321 cgccgatttg aatgccgcgg tggattacgt ttccaaactg ccgtcatgca atggcaaggt
     4381 tgtcgtgatg ggattttgct ggggcggcgg gcaggcgttc cgattcgcga cgaacaacaa
     4441 ggacatcaag gccgcgtttt cattttacgg gccgattccc gacgaatcaa cgttctcgcg
     4501 catccaatgt cccgtgtacg gtttttatgc cgagaaagac gcccgcatca ccagcactgt
     4561 tccttcggct gctgaagcaa tgcaaaaggc cggcaaaact tacgagacgg tcgtctatga
     4621 aggcgctggt cacggtttca tgcgcgcggg tgaggcgccg gacgcaaccg aagccaacaa
     4681 gaaagcgcat gaccaagcct ggcaacgcgc caaagaatta ttgtcaaaga tcaattgagc
     4741 caaatcagca cttcgactat tcccattcgc acaacgattc gacgtgcgca gccgagccat
     4801 cacgcagcca tccgtcaagc tgggcttgct ccttgagttg ctgtccgcgc aatcgcggca
     4861 ccaccacgta acggcaatcc gcctccgcca gcgcgctcat gtcgccccac aatttttcaa
     4921 attgcgcgac tgggaaaata tcttcctggc cctggcccca acgttttttg acctccttga
     4981 gcgtgatgta agtcggcatt cgcacggcca aagccctgat tgcggcgctc actttttccg
     5041 aatcttccaa ttcacccggc gtcagcccga aaagcgcgag cagcttctcg atgtcaatcc
     5101 aggtgctcaa ggtgttgtaa tacgaaaggg caaattcatc ctcttcgcgc ggcatggtaa
     5161 ggccctccaa aatgcggacg cgtccgttca cgcgcgcgag tccgccgccg cgatcttcaa
     5221 tccgccgcgt gatgacctcg aaggaaaggc acgcgccctg gcgtatgtgc agtccaagca
     5281 gcgccgggtc cacatccacg ccgagcgtgt caatgttatg caacagaaga tattttaatt
     5341 tcggacgttc gcgcaccaat cgggccagtg tgccattgcg caacagattg gaaatttcgt
     5401 accagtggcc gaccggatgg aggcattgcg cgggcaggtt gtcggtgtaa tcgttcccct
     5461 cgcccgcctg cgcccagccg ataagcgccg catgaaggct ctcgcggact ttctgcgcct
     5521 gttcatcaag caattgttgc ggcatttcct cccataaaaa acgaagatcg cgcaccatcg
     5581 gaaccatgcg caaaccaatg gccttgcccg gcgaaagcag cagcggaccg ctgtagccgt
     5641 aatttttttg gcttgcgaga aatgattcta tcgtcccgtg cgtcaggtaa ctggtggtga
     5701 cgacatgcgg cagggttgcg ccaaattcgc gattcacgcg ccggcttttg gcgagatgaa
     5761 cttccaaaaa tgtgcgatgc cgcccggcaa gtttgcaaaa cggatgcaac gccttcacca
     5821 cgcccgcgcc ctgcgtccag cggctgccga caccgccagc cagcgtcacc acggcgactt
     5881 cgccattgga aatggcagcc ttgccggtcg ttgcgtaatc cgcaggcaat cctcccgtgg
     5941 catccaacac gtcgtcagga tgcacatctt cgattttcgt gctggccggc aaacgatttt
     6001 gcgcaaggcc gatgcgtccg ttcttcaaat cggcgcgaat ctcctcatgc tgcgcgcggt
     6061 cgaacgcatt ttgttcgagc aactcggcca ggctttggcc cgcggcggtt tcgtgctta
//
LOCUS       DASZMM010000562         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2172914, whole genome shotgun sequence.
ACCESSION   DASZMM010000562 DASZMM010000000
VERSION     DASZMM010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2172914"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VG754_13495"
     CDS             <1..251
                     /locus_tag="VG754_13495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF20278.1"
                     /translation="KLGYVMAQQGDIEAAIPHFQKAITLESTNADAYFDLGAAYAALE
                     NWRPAVDAFSNAAQLKPGSAEFKGRLAEARAKASGAKK"
     gene            complement(280..1083)
                     /gene="trpC"
                     /locus_tag="VG754_13500"
     CDS             complement(280..1083)
                     /gene="trpC"
                     /locus_tag="VG754_13500"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWF20279.1"
                     /translation="MNILDKIVAQKKVEIARLPERTVSSSDLKAALQARGGIRSFENA
                     LQNPRRGKMALIAEVKKASPSAGIICADFDPVRIAKQYEAAGADCLSVLTDEQFFQGS
                     LEYLKQIRQAVKLPLLRKDFIIDERQILEAIEWGADAILLIVAILTDAQLKNFHALVT
                     EAGLTALVEVHDEAELNRALAAGAKVIGVNNRDLKSFKVDLATTERLAAKLHGAPNGS
                     EKLLVAESGIHTRADVERLAKCGAQAILVGESLMKHQDISGKIAELLGG"
     gene            complement(1090..1506)
                     /locus_tag="VG754_13505"
     CDS             complement(1090..1506)
                     /locus_tag="VG754_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20280.1"
                     /translation="MNIIDKIVAQKKVEIARLPQRNVSVSDLNAFQTPGTYNPNGVAA
                     QSPRLRGTRYLGNDNGKINNLEEVVSLDQKHGHNLFEVEHIVSPLPRVGSCEPTPGFE
                     TKPLRGFPIKRAKLLFCLDEFEISNPLFSGFPAFLI"
     gene            complement(1516..2208)
                     /locus_tag="VG754_13510"
     CDS             complement(1516..2208)
                     /locus_tag="VG754_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20281.1"
                     /translation="MKSKKIKGKRPMLWSKKSLISKDLLGFDSLFRSWIKVQRSYFNA
                     FEPKLCETSFGYRERPQVSLLGTAVFLSKGVCLQEYQLFKKQNKRVGRNDLWLQLRPG
                     YPKYEYLTEAKFDWIGLEKDYKERFEKLINSSTDSVKKLSSNPAGEKPRRLALAFGAL
                     TYPNKRIVSEADREREKFFESLDRACKKHKIDGWAGIWVNAAEFFSSLNLQKGHTAVG
                     LVMLAKSIDPAY"
     gene            complement(2237..>3100)
                     /gene="prfB"
                     /locus_tag="VG754_13515"
     CDS             complement(2237..>3100)
                     /gene="prfB"
                     /locus_tag="VG754_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_163967019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWF20282.1"
                     /translation="LKHEKDLERDLAQFSKDLENLELKVFLNGPHDKNNCILSINAGA
                     GGTESCDWANMLLRMYQRWAESRGWQVEINDALPGETAGIKSVTMLITGENAYGFCKA
                     ERGVHRLVRISPFDSNKRRHTSFASVDAIAEIEEESEVPIPPTEFQVDTFRSGGKGGQ
                     NVNKVETAVRITHIPTGLIVASQSQRSQHQNRATAMKLLLSRIFAQRIDAQKQEMERF
                     YGEKGSVSWGNQIRSYVFQPYRMVKDLRTGVETSNVQGVMDGDLDPFVNGWLRAGCPA
                     KRMQGIKDEEE"
BASE COUNT          702 a          876 c          732 g          790 t
ORIGIN      
        1 tcaagctcgg ctacgtcatg gcccaacaag gcgacatcga ggcggccatt cctcattttc
       61 agaaggccat cacgctcgaa tccaccaatg cggacgctta ttttgatttg ggcgccgctt
      121 acgccgcctt ggaaaattgg aggccggctg tggatgcctt ctccaacgcc gcgcaactca
      181 aaccgggcag cgcggaattc aagggccgcc ttgccgaagc ccgggcaaaa gcttccggcg
      241 caaaaaaata aaatgtcaaa gagcagggga aggtgttgtt caccctccca gtaattccgc
      301 gattttgccg ctaatgtcct gatgcttcat cagcgattcg ccgaccaaaa tcgcctgcgc
      361 gccgcatttg gcgagacgct caacatcagc gcgggtgtga atgccacttt cggcaaccag
      421 caatttttcg cttccgttgg gcgcaccgtg caatttggcc gccaatcgtt cggtcgtggc
      481 aaggtccacc ttgaaacttt tcaggtcgcg gttgttcact cctatgactt ttgcgcccgc
      541 ggccaacgcc cgattcaatt cagcttcgtc atgcacttcc accagcgcgg tcagtcccgc
      601 ttctgtgacg agcgcatgaa aatttttcag ttgagcatcg gtcaagatcg ccacaatcag
      661 caaaatcgcg tccgcgcccc attcgatggc ttcaagaatc tggcgctcat caatgatgaa
      721 atccttgcgc agcagcggca gtttcaccgc ctggcggatt tgcttcaaat attccagcga
      781 gccttggaaa aactgctcat ccgtcagcac cgaaagacaa tccgcgcccg ccgcttcata
      841 ttgtttggcg atgcgcaccg gatcgaagtc cgcgcaaatg attccagcag atggcgaagc
      901 ttttttgact tcggcaatca atgccatctt gccgcgacgc ggattttgca gcgcattttc
      961 aaagctcctg attccacccc gcgcctgcaa agccgccttc aaatccgacg acgaaacggt
     1021 ccgctccggc agacgcgcaa tctcaacttt cttctgcgct acgattttat cgaggatatt
     1081 caaggctttt taaattagga aagcaggaaa gccggaaaag agcggatttg aaatctcgaa
     1141 ttcgtccaaa caaaacagca attttgcgcg tttaatcggg aaacctcgaa gaggtttcgt
     1201 ctcaaagccc ggggttggct cgcaagagcc taccctgggt aacggcgaaa caatgtgttc
     1261 aacctcgaag aggttgtggc catgtttctg atccagagac acaacctctt cgaggttgtt
     1321 gattttgccg ttgtcattcc caaggtagcg agtgccgcgc aaccttgggc tttgggccgc
     1381 aaccccgttg gggttgtaag tgccaggcgt ctggaacgca ttcaggtcag agacagagac
     1441 gttgcgttgc ggcaaacgcg caatctccac tttcttctgc gctacgattt tatcgatgat
     1501 gttcaaggaa tgggttcaat acgctgggtc aatggattta gccagcatga caagtcctac
     1561 agccgtatgg cccttttgca aatttagact gctgaaaaat tccgcggcat taacccagat
     1621 tccagcccag ccatcaattt tgtgcttctt acaagctctg tccaggctct caaagaattt
     1681 ttctctctca cgatctgctt ctgaaacaat tcttttattt ggataagtaa gtgcaccaaa
     1741 cgcaagagcc aatcgtctgg gcttttcacc cgcagggtta gatgaaagct ttttgactga
     1801 atcggttgag gaattaatga gcttttcaaa tctttctttg taatcctttt ccagtccgat
     1861 ccagtcaaat ttcgcttcag tcaggtactc gtatttcgga tagccaggtc ttaattgaag
     1921 ccataaatca tttcttccta cacgcttgtt ctgttttttg aaaagctgat attcttggag
     1981 gcaaactccc ttggacaaaa aaaccgccgt tccaagcaat gatacttgtg gacgctctcg
     2041 atatccaaaa cttgtttcac agagtttcgg ctcaaatgca ttgaagtaac tacgctgcac
     2101 tttaatccac gaacgaaaaa gtgaatcaaa accaagcagg tccttggata tgaggctttt
     2161 ttttgaccaa agcattggtc gcttgccttt aatcttcttg gatttcataa ttctgttttc
     2221 ctgatttcct aattgattac tcttcctcat ccttaatccc ctgcatccgt ttggccggac
     2281 aacctgcgcg caaccagcca ttcacgaacg ggtccaagtc gccgtccatc accccttgga
     2341 cattgctcgt ttccacgccg gtgcgcaagt ccttcaccat gcgatacggt tgaaagacat
     2401 aactgcgaat ctggttgccc cacgagacgc tccctttctc gccgtaaaac cgttccatct
     2461 cctgcttttg cgcgtcaatc cgttgcgcga aaatacgcga gagcaacagc ttcatcgccg
     2521 tcgcgcggtt ctgatgctgc gaccgctggc tttgcgacgc gacgataagg cccgtcggaa
     2581 tgtgcgtgat gcgcaccgca gtctcgacct tgttcacgtt ctgaccgccc ttgccgccgg
     2641 aacggaatgt gtccacctga aattcagtcg gcggaatcgg cacttcgctt tcctcctcga
     2701 tttccgcgat ggcatccacg ctggcaaaac tcgtatgccg ccgtttgttc gaatcgaatg
     2761 gcgaaatgcg caccaagcga tgcacgccgc gctcggcttt gcaaaagccg tacgcattct
     2821 cgccggtgat caacatggtg acgcttttga tgcccgcggt ttcgccgggc agcgcgtcat
     2881 tgatttccac ctgccaaccg cgcgattccg cccaacgctg atacatccgc agcagcatgt
     2941 tcgcccaatc gcacgattcc gtgccacccg cgccggcgtt gatgctcagg atgcaattgt
     3001 ttttgtcgtg cgggccattg agaaaaacct tgagttcgag attttccagg tccttggaaa
     3061 attgcgccag gtcgcgctcc aaatccttct cgtgcttgag
//
LOCUS       DASZMM010000563         5137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5489534, whole genome shotgun sequence.
ACCESSION   DASZMM010000563 DASZMM010000000
VERSION     DASZMM010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5489534"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VG754_13520"
     CDS             complement(<1..253)
                     /locus_tag="VG754_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWF20283.1"
                     /translation="MTIDPRLHRIVAAQPYPLLFATISGAHLYGFPSPDSDFDLRGAH
                     VLPLEKIAGLVVENETVQDSRVIEGLEMDIVSHDIKKFFG"
     gene            complement(250..1143)
                     /locus_tag="VG754_13525"
     CDS             complement(250..1143)
                     /locus_tag="VG754_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWF20284.1"
                     /translation="MKPLVPAPKLQELINTISEKQLRDWVEVISKPRHYAVEPDQNRA
                     TAKWLSSVFNSIGLNVIVQGPYSNIVAMPKRTFKEAILIGAHYDSVPMCPGADDNGSA
                     VAAMLGCAAACSLWRPALPVIFVAFNREEDGLLGSKNFVETYLSELNFRINCAHILEM
                     VGFASSEAGSQRIPPGLPVKVSDKGDFLGLLANRHSNKPMDFVLCRAGAYTPDLPVMG
                     LDVKLGLEKYFPVLSRSDHAPFWSKKIPALMWTDTSEFRNNNYHRDCDTPDTLNYGFL
                     KHVTQLLTACVIQQAEENCSE"
     gene            complement(1185..2126)
                     /locus_tag="VG754_13530"
     CDS             complement(1185..2126)
                     /locus_tag="VG754_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWF20285.1"
                     /translation="MAHQSDNISAGTQIVSLVEVRGTNNSLVHPRGAVGVITRTPASV
                     SERHFLVRFPDGFEASLTREQIEVLKHFKDRLPGAESAAQFELESFIIYRCVVGSRAY
                     GLDNDESDTDRRGIYIAPAEMQWSLFGAPEQFEDNASQSCYWELQKFLTMALKANPNI
                     LECLYSPIVEKISPLGEELVAQRHAFLSQMIFQTFNGYAMSQFKKIEQDIRNHGEVRW
                     KHAMHLLRLLFSGAATLRTGAVPVRVEDESLRNRLLTVKRGELPWAEVDNWRKELHRD
                     FENALAKTQLPERPDYEVANRFLTKARRQIANQIFSA"
     gene            complement(2561..2914)
                     /locus_tag="VG754_13535"
     CDS             complement(2561..2914)
                     /locus_tag="VG754_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20286.1"
                     /translation="MNIAERGSSTRARVLEEALAKASGRAGHRWASEDLKGELVFNVQ
                     TPLREPLLAVPDYLGWAVQRVFEKGQTRFYDYLGEKIRLVVDLYDAEKYAGNRNYYDH
                     QNPLTVQNKIDPPST"
     gene            complement(3048..3671)
                     /gene="gmk"
                     /locus_tag="VG754_13540"
     CDS             complement(3048..3671)
                     /gene="gmk"
                     /locus_tag="VG754_13540"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWF20287.1"
                     /translation="MADPNSNPLLVVISAPSGGGKTTLCKQLLAKHPDMARAVTCTTR
                     VPREGERDEVDYYFLDATSFLKRVQAGNFLEHATVFGNSYGTLKSEVVSKLRQGKDVL
                     LNVDVQGAATIRERAEEDPELKQALVQVFLTPPTLGILEQRLKRRGTDSAQVIQKRLG
                     VARQEIAQWKNFDYLIISTSIAEDLRRMECIIEAEKMRVARSEPLEM"
     gene            complement(3675..4556)
                     /locus_tag="VG754_13545"
     CDS             complement(3675..4556)
                     /locus_tag="VG754_13545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWF20288.1"
                     /translation="MKSMTGYGRGECAQKGFKVTVELSSVNRKQSEISVALPRELEVL
                     EAQLRDVINRSISRGRLTARVALHSADGRLPGQVRLNVPLAKAYVRELNQLAKDLKLD
                     GPITLDMLIRVPGVVQTDEEMADAEDFWSAVEKALQQALATLVKMREREGAHLAKDLA
                     RRIDLMRRATTQVQKRAPEVQKRYREQLLLRIKSAGVETPGDDDERLLKEVVYFADRS
                     DISEELTRLQSHFQQFDDCLNSKEPVGRTLDFLAQEINREVNTIGSKANDSLISRAVV
                     TLKAELEKFREQAQNVE"
     gene            4902..>5137
                     /locus_tag="VG754_13550"
     CDS             4902..>5137
                     /locus_tag="VG754_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20289.1"
                     /translation="MAEIAVVDLKGLRLLKSFNKTLRRVCRRKRHRRVGADAHHRRQR
                     AEYLSLYLFGLLNPVVRSMRALCELGRTQRAKECL"
BASE COUNT         1122 a         1339 c         1431 g         1245 t
ORIGIN      
        1 tgccgaagaa ttttttgatg tcgtggctca cgatgtccat ctccaaacct tcaatgacgc
       61 gcgaatcctg gaccgtttca ttctcaacca ccaatccggc aatcttttcc aacggcaaca
      121 catgcgctcc ccgcaaatca aaatccgaat ccggcgacgg aaacccataa aggtgcgcgc
      181 cgctgatggt ggcaaacaac aacggatacg gctgcgccgc gacgatgcga tgaaggcggg
      241 ggtcaatggt cattctgaac aattctcctc ggcctgttgt atcacacaag cggtcagtaa
      301 ttgggtgaca tgcttgagga atccgtaatt cagggtatca ggagtgtcgc agtcacggtg
      361 ataattattg tttctaaatt cggacgtgtc agtccacatc aaagctggaa ttttttttga
      421 ccaaaaaggg gcatggtcac tgcgcgagag tacaggaaag tatttttcca atcccagttt
      481 tacatccaat cccatgacag gtaaatccgg ggtatatgca ccagcccggc acagaacaaa
      541 atccatcggt ttatttgagt gacggtttgc cagcaaccca agaaaatcac ctttgtcact
      601 gactttgaca ggcaacccgg gtggtatgcg ttgggaaccc gcttcggaac tggcaaaacc
      661 gaccatttcc aaaatatgcg cgcaattgat gcggaagttt aattctgaaa gataagtttc
      721 cacaaaattc ttgcttccaa gcaaaccgtc ctcttcgcga ttgaacgcta caaaaatcac
      781 cggcaaagcg ggacgccaca aactacaggc tgccgcacaa cccaacatgg cagccacagc
      841 actgccgttg tcgtccgcgc cggggcacat gggaaccgag tcatagtgag cgccgattaa
      901 tatcgcctct ttgaatgtcc gtttcggcat tgccacgata tttgaatagg gcccttggac
      961 gatcacattt aaacctatcg aattgaaaac agaagatagc cactttgcgg tagcgcgatt
     1021 ttgatctggt tcaacggcat agtgtctcgg tttggatatg acttccaccc aatcgcgaag
     1081 ttgtttttcc gaaatggtat tgatgagttc ttgcagtttt ggcgcaggaa ccagaggttt
     1141 cattctccgt gaatcagaaa tcatcataat ttaatttttg ggaatcacgc actgaaaatt
     1201 tggtttgcta tctgccgcct tgccttggtc aaaaaccggt tcgcaacttc ataatccggt
     1261 cgttccggta attgtgtttt tgccaatgca ttctcaaaat cgcggtggag ttcctttcgc
     1321 caattgtcca cttccgccca gggcagttcg ccgcgtttga ccgtcaggag tcggttgcgg
     1381 agggattcat cttccacgcg cacaggaact gcgccggtgc ggagcgtcgc cgcgccgctg
     1441 aacaggagtc gcaacagatg catcgcgtgt ttccagcgca cttcgccgtg attgcggatg
     1501 tcctgctcga ttttcttgaa ctggctcatg gcgtagccgt tgaaagtctg aaatatcatt
     1561 tgcgaaagaa aagcatggcg ttgcgccacc aattcttcgc ccaaagggga tattttctca
     1621 acaatcgggg aataaaggca ttcgaggatg ttcggattcg ccttcagtgc catggtcagg
     1681 aatttttgca gctcccaata gcaactttgc gacgcattgt cctcgaattg ctcaggtgcg
     1741 ccgaagagcg accattgcat ttctgcaggc gcgatgtaaa tgcctcgccg atcggtgtcg
     1801 gattcgtcat tgtccaatcc atacgcgcgt gaaccgacaa cgcaccggta gatgataaaa
     1861 gattccaatt cgaactgtgc ggcggattcc gcgccgggca gacggtcttt gaaatgtttc
     1921 aacacctcga tttgctcgcg ggtgagcgat gcttcaaagc catcgggaaa acggacaaga
     1981 aaatgccttt cggataccga agccggtgtg cgggtgatga cgccaaccgc gccgcgcggg
     2041 tgaacgagcg aattgttggt cccgcggacc tcgacaagtg acacaatctg tgtgccggct
     2101 gaaatattgt ctgactggtg cgccatcctg aaaaagtttg gcgcgaatat gtcgtagttt
     2161 aaaggttaaa ttttgggtta tgcagggacc atcgcccgct cccaatcctg aagggttgga
     2221 gcactcagcc cggggttgcg cgaatgcgct atcacaaatc cgattctcac gttgtcacgg
     2281 cttgtcacgg gtattctcac tattctcgga taaaccgggc taaaaaaaat aatatttgcg
     2341 tagtcaagaa aacgattcgg catccagcat ccagaaacca gcatcaagca tcggttcgct
     2401 tgcttcgtca aatctccagc ggctcggagc gggcgacgcg catctgtttt tgccgtgttt
     2461 aaatgtgttt ataattgttt acaagccttt ataagcgctt atatttgttt tggcctgctg
     2521 ccactgaaag tcgtctccat ggcgtcgcgg ggtatcccgc tcaggtggac ggtgggtcaa
     2581 ttttattttg gaccgtcaat ggattttgat ggtcgtaata gttcctattc cccgcgtatt
     2641 tttcggcatc gtatagatca acgacgaggc ggattttttc gccgagataa tcgtaaaaac
     2701 gtgtctggcc tttttcaaag acgcgctgga cggcccatcc aaggtagtca gggacggcga
     2761 gcaatggttc ccgcaacggc gtttgcacgt taaaaaccaa ttcacctttc aaatcttcgg
     2821 atgcccaacg atggccagcg cgccctgaag ctttggcgag agcttcctcc agcaccctgg
     2881 cgcgggtgct tgagcctcgt tcggcgatat tcaatacgat ccggcgtggt tttttgagcc
     2941 tgcttttgat aaggtgcgac agcacgtccg cataaaattc atcatccttg ccgtggtgct
     3001 tgtttatgaa cagggagtga atctttcgag cgatgataat ttcggcctca catctccagc
     3061 ggctcggagc gggcgacgcg cattttctcg gcttcgatga tgcattccat ccggcgcaaa
     3121 tcttccgcga tgctggtgct gatgatgagg taatcgaaat tcttccattg cgcgatttcc
     3181 tgccgggcga cgccaaggcg tttctggatg acttgcgcag aatcggtgcc gcggcgtttg
     3241 aggcgttgtt ccaaaatgcc cagggtcggc ggcgtgagaa aaacctgcac gagcgcctgc
     3301 ttcaattccg ggtcttcctc ggcgcgttcg cggatggtcg cggcgccctg gacatcgacg
     3361 ttcaacagca cgtccttgcc ctggcgcaat ttgctgacga cctcggactt caaggtgccg
     3421 tagctgttgc cgaaaacggt ggcgtgttcg agaaagtttc cggcctgcac gcgtttcaag
     3481 aacgacgtgg cgtccaaaaa ataataatca acttcgtcgc gctcgccttc gcgcggcacg
     3541 cgcgtggtgc aggtgacggc gcgggccatg tcgggatgct tcgcgagcaa ttgtttgcaa
     3601 agcgttgttt tgccgccgcc ggatggcgcg gaaatcacga cgagcagggg attggaattg
     3661 gggtcggcca tggattattc gacgttctgc gcctgttcgc ggaatttttc cagctcggcc
     3721 ttgagcgtga cgacggcgcg cgagatgagg ctgtcgttgg ccttggagcc gatggtgttg
     3781 acctcgcgat tgatttcttg cgcgagaaaa tcgagcgtgc gtccgaccgg ctccttggaa
     3841 ttgaggcaat cgtcgaactg ctggaaatga ctttgcagcc gcgtcaattc ctccgagatg
     3901 tccgagcggt cggcgaaata tacgacctcc ttgagcaaac gctcgtcatc gtcgccgggc
     3961 gtttcgacgc cggcggattt gatgcgcaaa agaagttgtt cgcgatagcg tttctggact
     4021 tcgggcgcgc gtttctgcac ctgcgtcgtg gcgcggcgca tgaggtcgat gcgccgggca
     4081 aggtccttgg cgagatgcgc gccttcgcgt tcgcgcatct tgacgagcgt tgcgagcgct
     4141 tgttgcaacg ccttttcaac cgcggaccag aaatcctcgg catcggccat ctcctcgtcc
     4201 gtctgcacaa cgccggggac gcgaatcaac atgtcgagcg tgattggccc gtcgagcttg
     4261 aggtccttgg caagctgatt caattcgcgc acgtaagctt tcgcgagcgg aacattgaga
     4321 cggacttgcc cgggaagcct gccatcggcg gaatgcagtg cgacgcgcgc ggtgagacgt
     4381 ccgcgggaaa tggagcggtt gatgacgtcg cgaagctggg cttcgagcac ttcgagttcg
     4441 cggggcaagg ccacggaaat ttcactctgc ttgcggttga ccgagctgag ttcgaccgtg
     4501 accttgaaac ctttctgggc gcattcgccg cgcccgtaac ccgtcattga tttcatttga
     4561 aggatatagc atggccgatg gcaacgtgac aggcgaatct tttttgaaat gacaaacttg
     4621 cttggcgcat ccgcgcactg ccacgatttg ttggccatcg cgcctcgcgt tcaccgggta
     4681 aaacggagcg cggctcgatg agccgcagca acgcgcgacc aacaggatat gccgggctaa
     4741 ttcatgtgcg cccatccttc ccacattgct gcgggtcacc gaccaatagt gtccggcaca
     4801 atgaatgctc ggtaattttg tgggggatga cacagtgatg taacaggcct gtcacgggat
     4861 tgtaacttgt acaacgctgg tggtcgcgct ttaatcaacg cgtggccgag attgcggtgg
     4921 tagatctgaa gggtttgcgc ctgctcaaga gttttaacaa aactctgcgg cgggtttgcc
     4981 ggcgcaaacg gcaccggcgc gtcggggcgg atgcccacca tcggcgtcag cgagccgagt
     5041 atttaagtct ctatcttttt gggttgctca atccggtggt gcgcagcatg cgcgcgctgt
     5101 gcgaactggg ccggacgcaa agggcgaagg agtgcct
//
LOCUS       DASZMM010000564         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2173104, whole genome shotgun sequence.
ACCESSION   DASZMM010000564 DASZMM010000000
VERSION     DASZMM010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173104"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VG754_13555"
     CDS             <1..584
                     /locus_tag="VG754_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF20290.1"
                     /translation="GVIEALDKAKQQGKTRFVGFTGHKNPAFHLEMLKRGFPFDTVQM
                     PLNAFDATFHSFEQQVLPEVNRRGMAALGMKSMGGTADAVKKGVVKAEELLRYAMSLP
                     VATTISGMDSLKVLEQNLRVARGFKPMTPEEMDTLRQRCAPMAADGRFEPYKASLKFD
                     NPLTRLPHGFPIDDQQKEVKAMAEKGYGTWVPI"
     gene            599..1681
                     /locus_tag="VG754_13560"
     CDS             599..1681
                     /locus_tag="VG754_13560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF20291.1"
                     /translation="MKTQENYPDVVPNPQRRRFVQAGLAGAGAILAESLFSSPGIAAP
                     PPLQMTNTGADSIPRKPMGRTGEQLSIFGLGGYHLGTLDTADDAAKIVHEAIDAGINF
                     FDNAWEYNDGRSEEFMGKALQGRRDKIFLMTKVCTHGRGKEVAMQQLEQSLKRLRTDH
                     LDLWQIHEVIYYNDPQLIFAKDGVIEALDQAKKDGKVRYVGFTGHKNPAIHLEMLSHD
                     YPFDTVQMPLNCFDGTFRSFEQQVLPEVQRRGMAALGMKSLGGDGQPLLHGVVEVEKA
                     FRYAMSLPVATTISGVDSLAVLRQNLAIARGFKPMPPEEMQALRNRCAPFAADGHLEL
                     YKVTMKYDADVGREQHGYPSQEKLPI"
     gene            1918..>3639
                     /locus_tag="VG754_13565"
     CDS             1918..>3639
                     /locus_tag="VG754_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF20292.1"
                     /translation="MKAIQPGTRRDALLFTARLILAVSIFSLFAAFAPSLRADPPLPA
                     TANPTNPPPEITNSATPFADEGASAYKKLSLEQLMNLDVTSVSRAPEPFAQAPAAIEV
                     ITSDEIRRSGATSIPEALRLADNLEVAQINSHDWAISARGFNSGLGNKLLVMIDGRTV
                     YSPLYSGVFWNVQDYLLEDLDRIEVVSGPGGTLWGANAVNGVINIITKSAKDTQGLYI
                     ESGGGVPGTTGGVADARSEPRDFTGIRYGGTLASNVYFRIYGKAFQRGNEDYPNGSPA
                     NDRWEMAQGGFRMDAEASSSDNFTLQGDIYGSDLRIPAGGLANQSGGNLLGRWSHVIS
                     ENEDMSLQLYYDRTHLYDPISGFGTTQIVTDDLGTYDMDFQYRLQAGERNQVTWGLGY
                     RFTHDVVSESTILQFLPPVLDRHLYSSFVQDEIKLTDHLFFTMGTKLEHNDYTGFEIE
                     PSGRLQWNVTSNHMLWSAVSRAVRTPSRVDADIRENNPVPLITFLRGTPGFESEALIA
                     YELGYRSQITPKFSTSLSTYYNDYTDLRSVEITPGGLLGVVPLHYANDMEGHTYGMEL
                     SMDYQVLE"
BASE COUNT          837 a          973 c         1043 g          786 t
ORIGIN      
        1 acggtgtcat cgaggcgctc gacaaggcca agcaacaggg caaaacgcgc ttcgtcggtt
       61 tcaccggcca caaaaatccc gcgttccatc tcgaaatgct caagcgcggt tttccgttcg
      121 acacggtgca gatgcccttg aacgcgttcg acgccacgtt ccacagtttc gagcagcagg
      181 ttttgccgga agtgaatcgt cgcggcatgg cagcattggg catgaagagc atgggcggca
      241 ctgccgacgc ggtgaaaaag ggcgtcgtca aggccgagga attgttgcgt tatgcgatga
      301 gccttccagt cgcaacgacc atcagcggca tggattcgtt gaaggtgctc gagcaaaatt
      361 tgcgtgtcgc gcgcggattc aagccgatga cgccggagga aatggacacc ttgcgccagc
      421 gttgcgcgcc gatggccgcc gacggacggt tcgagcctta caaggcttcg ttgaaatttg
      481 acaatccgct gacgcgcctg ccgcatggct tcccgattga cgaccagcaa aaggaagtca
      541 aagcaatggc ggaaaagggt tacggaactt gggtgccgat ttgaatcgtg aaagaaacat
      601 gaaaacacag gaaaattacc cagacgtggt gccgaatccg caacgccgtc gcttcgtgca
      661 ggcgggtttg gccggcgcgg gcgcgatttt ggcggaatca ttgttcagca gtcctggcat
      721 cgccgcgcca cccccgttgc aaatgaccaa caccggcgct gacagcattc cgcgcaagcc
      781 gatgggccgc accggcgagc aactttcgat ttttggattg ggtggttatc acctgggcac
      841 gctcgataca gcggatgatg ccgccaaaat cgtccacgaa gcgattgatg cgggcatcaa
      901 ttttttcgac aacgcctggg aatacaacga tggccgcagc gaggaattca tgggaaaggc
      961 attgcaggga cgccgcgaca agattttttt gatgaccaaa gtctgcacac atggacgcgg
     1021 caaagaggtt gcgatgcaac aattggaaca atcgttgaaa cgcttgcgca ccgaccatct
     1081 cgatctctgg caaatccatg aagtgattta ctataatgat ccccaactca tcttcgcgaa
     1141 ggacggcgtg atagaagcgc tcgaccaggc caaaaaagat ggcaaggtcc gttacgtcgg
     1201 gttcaccggg cataaaaatc ccgccatcca tctcgaaatg ctttcgcacg attacccgtt
     1261 cgacactgtg caaatgccgc tcaattgttt tgacgggacg tttcggagct tcgagcagca
     1321 ggtgttgccc gaagtgcagc ggcgcggcat ggcggcgttg gggatgaaaa gcttgggcgg
     1381 tgacggccag ccattgctgc acggagtcgt cgaagttgaa aaagcatttc gttacgcgat
     1441 gagtttgccc gtcgccacca cgatttcggg ggtggattcg cttgctgtgc ttcggcaaaa
     1501 cctcgccatc gcgcgcgggt tcaagccgat gccgcccgaa gagatgcaag ccttgcgcaa
     1561 ccgttgcgct ccgttcgccg ccgacggtca tctggaatta tacaaggtca ccatgaaata
     1621 cgacgccgac gtcggacgcg aacaacatgg ttatccgtcg caggaaaagt tgcctatcta
     1681 aaggggattc ttaatcccgg atgaacaccg atggacgcgg atctggaaat caaaattgaa
     1741 ttccacgaag aacctgaaga taattccgct tgtcagccac aaatttcttg tgaatttcgg
     1801 attgctgcgt aacatgtccc aatcctgcag gttcgatagc ggcgcagtta tcgtgtattc
     1861 atgggcgctc aaaaattttt gcatgggtac cagtggtctg cgtggacacg ggctgccttg
     1921 aaagccattc aaccgggaac ccggagggac gcacttcttt tcaccgcacg actcatcctc
     1981 gccgtaagca ttttttccct ttttgcggcg ttcgcgccaa gcctgcgcgc tgacccgccg
     2041 ttgccagcca ccgccaaccc gacgaatccg ccgcccgaaa tcacgaattc ggccacgcct
     2101 ttcgctgacg aaggcgcttc ggcctacaag aagttgagcc tggaacagtt gatgaacctg
     2161 gacgtcactt ctgtttccag ggctccggag ccgttcgcgc aagcccccgc tgccatcgag
     2221 gtcatcacgt ccgatgaaat ccgccgctcc ggcgcgacca gcattcccga agcgttgcgg
     2281 ttggccgaca atttggaagt ggcgcaaatc aattcccatg attgggcaat cagcgcgcgc
     2341 ggattcaatt cggggcttgg caacaaactt ctcgtcatga ttgacgggcg caccgtgtat
     2401 tcgccgctgt attcaggggt gttttggaac gtgcaggatt atttgctgga agatttggac
     2461 cgtatcgagg tcgtcagcgg acctggcgga acgctttggg gcgcgaacgc ggtcaatggc
     2521 gtcatcaaca tcatcaccaa aagcgcgaag gacacgcagg gattatatat cgaaagcggc
     2581 ggcggtgtgc cgggaacgac tggaggagtc gcggatgcgc gttccgagcc gcgggatttc
     2641 acgggcattc gttacggcgg aacgctcgct tccaatgttt atttccgaat ctatggcaaa
     2701 gctttccaac gcggcaacga agattacccc aatggcagtc ctgccaatga caggtgggaa
     2761 atggcgcaag gcggtttccg catggatgcc gaggcatctt cctccgacaa tttcaccttg
     2821 caaggggaca tctacgggag cgacttgcgc attccagccg gcggtctggc aaatcagagc
     2881 ggcggcaatc ttttgggccg ttggtcgcat gtgatttcag agaatgagga catgagtctg
     2941 caattgtatt acgacaggac gcatttgtac gacccgatca gcgggtttgg aacgacccag
     3001 attgtgaccg acgatttggg gacgtatgac atggattttc agtaccggct gcaggcgggc
     3061 gagcgcaacc aggtgacgtg gggcctcggc tatcgtttca cgcatgacgt ggtcagcgag
     3121 tccaccatcc tgcagttttt accgccggtg ctggaccgcc atctgtacag ctcattcgtg
     3181 caggacgaaa tcaagctcac agaccatctt ttcttcacca tgggaacgaa gctcgagcat
     3241 aacgattaca ccggtttcga aattgaaccg agcggacgtc tgcaatggaa tgtcacttcc
     3301 aatcacatgc tttggagcgc ggtgtcgcgc gcggtgcgaa ccccttcgcg ggttgatgcg
     3361 gatattcgcg agaataatcc agtcccttta ataacattct tgaggggaac acccggtttt
     3421 gaatccgaag cgctgattgc gtatgaactt ggctatcgat ctcagatcac gccaaaattt
     3481 tccacgtctc tttcgactta ttacaatgat tacaccgacc tgcgcagtgt ggagatcact
     3541 cctggagggt tgctgggtgt cgtgccatta cattatgcga acgatatgga gggccacacg
     3601 tacggaatgg aattgagcat ggattaccag gtgcttgag
//
LOCUS       DASZMM010000565         3526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4650800, whole genome shotgun sequence.
ACCESSION   DASZMM010000565 DASZMM010000000
VERSION     DASZMM010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4650800"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VG754_13570"
     CDS             complement(<1..390)
                     /locus_tag="VG754_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20293.1"
                     /translation="MINLSKLAVCSWSLRPTDPQNLIQQLQSISINRVQIALDPLREN
                     PSVWGALPELCATHGITFVSGMLVTVGEDYSTLESIKRTGGVVPDATWDENWRNMQSA
                     AVLAKKLGLKLVTFHAGFLPHEPGDPTF"
     gene            complement(409..957)
                     /locus_tag="VG754_13575"
     CDS             complement(409..957)
                     /locus_tag="VG754_13575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWF20294.1"
                     /translation="MITLIVGTNRPESNTRKVARQVEEIYAQLKAPLRVLDLAELPPE
                     IFLPTSYAEKPKSFAPFADAILSASGLVIISPEYNGSIPGVLKYFIDMLKFPESFEKR
                     PVCFIGLAAGVWGALRPIEQLQQIFSYRNAYIYPERVFLSQVRNLLDAEGRLNNPELA
                     GRLKTQAQGFIEFVKRFHPRAA"
     gene            complement(1016..1306)
                     /locus_tag="VG754_13580"
     CDS             complement(1016..1306)
                     /locus_tag="VG754_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF20295.1"
                     /translation="MSEFRVSATAREDLEEIWIYIAVDNSEAADRLIHRLVSQFRLLA
                     SMPGMGWQRDDLASGLRSFPLGNYVIFYRSQANGIEIVRVLHRARDFPPLFD"
     gene            complement(1303..1569)
                     /locus_tag="VG754_13585"
     CDS             complement(1303..1569)
                     /locus_tag="VG754_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20296.1"
                     /translation="MRTVEEIKAAAAALDPDEQVELFRWWVQTDVFKKRQLAALKKEI
                     AKGVDDLKDGRYQTYTDSNVMQLREDIGKSGRERLKAMNRKKRS"
     gene            complement(1612..2355)
                     /locus_tag="VG754_13590"
     CDS             complement(1612..2355)
                     /locus_tag="VG754_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWF20297.1"
                     /translation="MNKTLVIVPTYNEKDNLPPLVKRLLELPIPVELLVVDDNSPDGT
                     GKLADELAAKHPQIHVLHREQKQGLGRAYCAGFAWALEHGYEFIMEMDGDFSHNPDDI
                     PKFIGAAENADLVLGSRYKNGIRVINWPLRRLMLSTTAAKYVQFITGMPFTDPTGGFK
                     CFRRRALQSIDLNAIQSNGYSFQIELTHKIWRKGMRVEEVPIIFTDRIQGTSKMSGTI
                     VREALWMVWKLLLQNHFRRSPREQNPASN"
     gene            2478..3386
                     /locus_tag="VG754_13595"
     CDS             2478..3386
                     /locus_tag="VG754_13595"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF20298.1"
                     /translation="MGITGRAWISLACPVSRALLRLIEPRSVSPILSIAQNNRYSLIS
                     PGMVASILQYLFSAGSLIGLVPFILLFIFQVWMCVDAIRRGEWLWAVFIWFFPMINAA
                     LYYFFVYRASSPTRGFELPGAHNRKRIKELQAQIHHLDKAHHYFQLGDIYFQQGKLDK
                     AEAAYRASLERDPKDIDARAHLGQCLLREKKAAEARPLLEGVCAENPKHDYGYSMMAL
                     AETLTALGEPKAAIAIWRQVTESHQYPRAKVQLAELYVNDNQLDLARAELKDVLSDDV
                     HAPAFQRKRDRVWVRRARSLAGKIKS"
BASE COUNT          818 a         1012 c          889 g          807 t
ORIGIN      
        1 gaaagttgga tcgcccggtt catgcggcaa aaaaccggcg tgaaatgtca caagcttgag
       61 gccgagtttt ttggcgagca ctgcggcaga ttgcatgttg cgccaatttt cgtcccaagt
      121 cgcatcagga accacgccgc cggtgcgctt gattgattcc aatgtcgaat aatcttctcc
      181 cacggtcacc aacatgccgg agacgaatgt gatgccatgc gtcgcgcaca attccggaag
      241 cgcgccccaa acggaaggat tttcgcgcaa cggatcgagc gcgatctgga cgcgattgat
      301 gctgatggat tgcagttgct ggatgagatt ttgcggatcc gtcggcctca gcgaccaact
      361 gcacaccgcg agtttcgaaa ggttgatcat ttttttgcgg aaacccgttc aggcggcgcg
      421 cggatggaat cgtttgacga attcgatgaa gccctgcgct tgcgttttca agcgacccgc
      481 gagctcggga ttgttcaacc ggccttcggc gtcgagcaga ttgcggacct gcgacagaaa
      541 aacacgttcc ggataaatat aggcgttgcg atagctaaaa atctgttgca actgctcaat
      601 cggccgcaac gcgccccaaa cacctgccgc gaggccgatg aaacagaccg gccgtttctc
      661 aaaactttcc gggaatttga gcatgtcgat gaaatacttc aacacgcccg gaatgctgcc
      721 gttatattcc ggcgaaataa tgacaaggcc ggaagcgctg agaatagcgt cggcgaatgg
      781 cgcgaaagac tttggttttt cggcgtagga cgttggcaaa aaaatttccg gcggcaactc
      841 cgcaaggtcc agcacacgca acggcgcttt gagttgcgca taaatctcct cgacctgccg
      901 ggcgactttg cgggtgttgc tctccggacg gttcgtgcca acgatgagcg taatcatggc
      961 aaaagaatgg cgggcagccc actttcaggc aagcgtggaa tggatccgct cccaatcagt
     1021 caaataatgg aggaaaatcc cgtgctctgt ggagcactct gactatttcg atgccatttg
     1081 cctgtgaacg ataaaagata acgtaatttc caagtggaaa acttctcaat ccagaagcca
     1141 aatcgtcccg ctgccatccc atgccaggca tggacgccag caagcgaaat tgggaaacca
     1201 atctatgaat caaccgatct gctgcttcgg agttatcaac tgcaatataa atccagattt
     1261 cctccaaatc ctccctggct gtcgccgaaa cccgaaattc gctcatgacc gttttttgcg
     1321 attcatcgct ttcaaacgct cacgtcctga tttgccaatg tcctcgcgca attgcatcac
     1381 gttgctgtct gtgtaggtct gataacgccc atccttcaaa tcatcaacgc cctttgcaat
     1441 ctcctttttc aacgcggcaa gctgccgttt tttaaaaaca tccgtctgaa cccaccaacg
     1501 aaaaagttca acttgttcgt caggatccag tgctgctgcg gctgctttga tttcttccac
     1561 tgtgcgcata ctcaaaatta tcacgacaac gcgggacttg tccacacgtc gctaattgga
     1621 cgccggattt tgctcccggg gactccgtcg aaaatgattt tgcaacaata atttccagac
     1681 catccagagc gcttcgcgga cgatggtgcc ggacatcttg gacgtgcctt gaatacggtc
     1741 ggtgaaaatg atcggcactt cctcgacgcg cattccttta cgccagattt tatgcgtcag
     1801 ctcaatctgg aaactgtaac cgttggattg gatggcgttg agatcaatgg attgcaacgc
     1861 gcgccggcga aaacatttaa acccgcccgt cgggtccgtg aacggcatcc cggtgatgaa
     1921 ctgcacatat ttcgcggcgg tcgtgctcag catgagccgc cgcaacggcc agttgatgac
     1981 gcggatgccg tttttgtaac gcgagccgag gaccaaatcc gcgttctccg ccgcgccgat
     2041 aaatttcggg atgtcatcgg gattatgcga aaaatcgccg tccatctcca tgatgaattc
     2101 gtagccgtgt tccaacgccc aggcgaaccc ggcgcaatac gcgcgaccca acccctgttt
     2161 ttgctcgcga tgcagcacat ggatttgcgg atgcttcgcg gcaagttcat ccgccagctt
     2221 gccggttccg tcgggagaat tgtcatccac caccagcaac tcgaccggga tgggcaattc
     2281 cagcagccgt tttaccagcg gcgggagatt gtccttctcg ttatacgtcg ggacgattac
     2341 cagcgttttg ttcatcaggc aaagtccgct cacaatcaac caattgcctg ctgaacacaa
     2401 gccgatttgt tcccgcagcg caagcttgag cgcatccgcg aactgctccg gagcgcgggc
     2461 cggcggccct cagaaatgtg ggaattacag ggcgcgcatg gatcagtctg gcgtgtcctg
     2521 tttctcgcgc gttgctgcgg ctcatcgagc cgcgctccgt ttcgcccatt ttgtccattg
     2581 cacaaaacaa ccggtattcg ctaatctctc ccggcatggt ggcttcgata cttcaatatt
     2641 tgttctctgc cggaagtttg atcgggcttg ttccattcat cctgctcttc atttttcaag
     2701 tttggatgtg cgttgatgcc atccgccgcg gcgaatggct ttgggcagtg ttcatctggt
     2761 tttttccgat gatcaatgcg gcgctttatt atttcttcgt ctatcgcgcg tcgtcgccca
     2821 cgcgcggctt cgaactgccc ggcgcgcaca accgcaaacg catcaaggaa ctccaggcgc
     2881 aaatccatca cctcgacaag gcccaccatt attttcaact cggcgacatt tattttcagc
     2941 aaggcaagct cgacaaggcc gaagccgcgt accgcgcctc gctcgaacgc gacccgaagg
     3001 acattgacgc ccgcgcgcat cttggccaat gcctgttgcg cgagaaaaaa gccgccgaag
     3061 cgcgcccgtt gctcgaaggc gtttgcgccg aaaatcccaa gcacgattac ggctattcca
     3121 tgatggcgct ggccgaaacc ctcactgcgc tcggcgaacc gaaagccgcc atcgcaatct
     3181 ggcgtcaagt caccgagagc catcaatatc cccgcgccaa agtgcaactc gccgaacttt
     3241 acgtaaacga caaccaactc gatctcgccc gcgccgaatt aaaagacgtc ttgtccgacg
     3301 acgtccacgc gccagcattc caacgcaaac gcgaccgtgt ctgggttcgc cgcgcaagaa
     3361 gcttggcggg gaaaataaag agttaaatca accgacgcta tggcgtggtt tcgggcgcat
     3421 ccaatacgtc gcaccattta tcctccaaat aatcattcaa ctgcccaagc atgggaattt
     3481 ttacgcaata ttgcatggga gatgaatacc aatcatatgc gccatc
//
LOCUS       DASZMM010000566         7912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3551401, whole genome shotgun sequence.
ACCESSION   DASZMM010000566 DASZMM010000000
VERSION     DASZMM010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3551401"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..1603
                     /locus_tag="VG754_13600"
     CDS             452..1603
                     /locus_tag="VG754_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWF20299.1"
                     /translation="MLNLPEALTTQDSPEIAKIARLQYVTDSSKGFRRVASGKGFKYL
                     DMQGKTITEDATLRRIKGLAIPPAWKNVWICPVENGHLQATGCDDRKRKQYRYHPRWR
                     EVKDETKYAKMLAFAKALPKIRKRVAKDLRAPELSKRQVLATVVRLLEVSLIRVGNEE
                     YAHENHSYGLTTLKNHHAKVQKSTIQFHFRGKSGKLRQVEVHDARLARIVRECRDIPG
                     QDLFQYIDADGQPQQIRSEDVNDYLHEISGGDFTAKDFRTWAGTVLAGIALQEFQKFD
                     TKAQAKKNVVQAIESVATRLGNTPAICKKCYIHPEILEGYLQGATIQTIRARAETELK
                     KGLHQLQPEEAAVVAFLQKRLAEKEQPLEKILKRSLKRFKHPHLPGHQR"
     gene            1668..2087
                     /locus_tag="VG754_13605"
     CDS             1668..2087
                     /locus_tag="VG754_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HWF20300.1"
                     /translation="MNKSSKPRSQHDRFRHFAHAAANWMGNPWCFFFALVIIVAWGIT
                     GPLFHFSDTWQLIINTGTTIITFLMVFLIQNTQNRDAQAIHLKLDELIRAMQGARNQM
                     LNLEHLSDEELAELKRQFDKICKRATRSSGELADKLP"
     gene            2186..3145
                     /locus_tag="VG754_13610"
     CDS             2186..3145
                     /locus_tag="VG754_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF20301.1"
                     /translation="METQIPKRPAARFKGRLMLCNIGRVGDTILRNSILDSGFRTYST
                     VDYICGPNNVELIRADPRINRITVLRNSISGFASLLMTAARGRYDNFIELKDHWSSTS
                     VLITGFFRSRVKTGWNSDRFKPFHRDVRTVYEPQMHKIEMMRRVAELAGLEPGEYKAT
                     LVLPADSVRWFDENYKWKEPFIFLNISATSEDRMWPTMNWARYVRSCGLEGPILVNGV
                     PKDRDAVHQLCAELPGSIAFRPRQFMDVAAALNRARLVLTVDTGVVHACSALNRPIIA
                     LGHSGKEYSPLSTRTLMIRTRDCTVRQFDPELAIAETLRHGLP"
     gene            complement(3149..4081)
                     /locus_tag="VG754_13615"
     CDS             complement(3149..4081)
                     /locus_tag="VG754_13615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF20302.1"
                     /translation="MPATQAKILVIRGGAIGDFILTLPVLAALRQQFPRAELEVLGYP
                     HIAQLALAGGLASRVQSIEARPLAGFFARNGELAKELAEYFAGFQLIISYLYDPDEIF
                     KTNVGRCTRAQFIPGPHRPDEKDEVHATEVFLKPLERLAIFEADAVPRLDVTKAFETT
                     LTTGRWLAVHPGSGSENKNWPEAKWNELLQQLMSNTKLKLLLIGGEAERERLDRLARA
                     LPSTRIKIMKGAPLSELAQWLASCVGFVGHDSGISHLASAVGVRSLILWGETDEAVWR
                     PRGREMTMLRDSKGLEQLSVPVVLHHLEQLLKRD"
     gene            complement(4221..4775)
                     /locus_tag="VG754_13620"
     CDS             complement(4221..4775)
                     /locus_tag="VG754_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20303.1"
                     /translation="MHVQLSTLAIILGLLVGLPQIYGLMKPAAFAAGVRKFPRSLPWG
                     ITLMLLGTAWFVFYVNAESLSDFVAYKPVLLGGFAAVGIASCVYVQDFLAVRGLAVVM
                     LLLAKLMVDTARWANTEWNLVIVTWAYVLVIGGIWFTLYPWHLRDLLNWATANEKRVR
                     IGSAIRLAFGLFVAVLGFTVYRSA"
     gene            4862..5581
                     /locus_tag="VG754_13625"
     CDS             4862..5581
                     /locus_tag="VG754_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20304.1"
                     /translation="MACRMFGYCGTLESDVRELYSGLKRACANDPALQSVAPNCSQHS
                     HGWGYVIHADNGLFHYRTSRSIYEDDIVLPKLEGQIRAIFHGRYASNLELMGHIFSHP
                     FMAFNDKQTIFLAHNGGVDPKEKKISERKVDSEWALEQIIKAGDVASALPKLKEHTKS
                     ALNLLILTIDREKGTPALLQYFHYFKPKEPEKVKYYKMYEGAMPGGRALVSSTMTFSE
                     AKMGSLTNIEPAKFDELMKLG"
     gene            complement(5720..7072)
                     /gene="miaB"
                     /locus_tag="VG754_13630"
     CDS             complement(5720..7072)
                     /gene="miaB"
                     /locus_tag="VG754_13630"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWF20305.1"
                     /translation="MPSVYIKTYGCQMNERDSEAVAAQLVAKGYTLASSETVADVVLL
                     NTCSVRDAAEQKAIGKMQNLAAEVRKNRPNVVLGFMGCMAQSRGQQLVDQLPDVDLVI
                     GTQKFHRTADYLDEIFSGVREKIVDTAEEQGSEAAIKEHILNGKSAAKSVSAFVSIMQ
                     GCNQYCTFCIVPYTRGEERSRSIEGIVAECRELVARGVKEITLLGQIVTSYGKRDIPV
                     RDGKSAFVQLLEAVHEIEGLERIRFTSPHPKGYGDDLVEAYGRLPKLVESAHLPVQSG
                     SDRLLKAMHRGYTRERYMGIIKKLRHVRPRMGITTDIIVGFPGETEADFEETLSLARE
                     VEFDNAYIFKYSPRKDTPAATLSDQLLQAVKEERNARLLALINEIGARHYVAYVGGNV
                     EILVEGPSRKNPARLTGRTRCNKIVVFDGSSRHIGQVMDVKITRVGSFTLYGDPAILE
                     "
     gene            complement(7200..7592)
                     /locus_tag="VG754_13635"
     CDS             complement(7200..7592)
                     /locus_tag="VG754_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20306.1"
                     /translation="MAFNIRMGVPEMESFWNDLSARKQQGKLDKNEEKFFKKLVKALG
                     YLAENPRYNSLASHEIEDLTSKYGLKIFQSYLENNTPAAGRIFWAYGPDKRDITVLAI
                     EPHPEDQKRGAYQRIKLSSLPSAKSKDR"
     gene            complement(7601..7876)
                     /locus_tag="VG754_13640"
     CDS             complement(7601..7876)
                     /locus_tag="VG754_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006689659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20307.1"
                     /translation="MPTMTQKQALKVIENYDVAADAKKRISLRNAKTKYFHVKALSNG
                     CYLLEPRVLVPPQAVSDRSLKMLDKSVANLKKGMASAPINLAAFNED"
BASE COUNT         1898 a         2137 c         2086 g         1791 t
ORIGIN      
        1 cggcgtctcc gcggtgaaat tccatgcaga agaaaggtcg cgggtctttc cagacggggt
       61 gcttccgcac gggcattacg ctccccccac cctctgaaaa ctcacgtttt cagctacgag
      121 ggattgggcg ttcaagttgt aagtggttgt aaacccgctt aaacccgcat aggttcgcct
      181 aaacccgccg aaaaatggca cccctcctgc ggggaagttc aaagtttaaa gtttaaggtt
      241 caaagttggg ttaggatttg tggattgcgg agtgcggaat gaagaactcg acattcagca
      301 tcaagcatcc agcatcgggt ttgttaaccc taattaacgc taattaacgc taattaacgc
      361 taattaacgc taaaatgaga aaaaattttt cccggatgat gtcggaccct gggttttttg
      421 aatgtggcgt gaagcggtgc taggttttcc catgctaaac ctgcctgaag cgctcacgac
      481 ccaggacagc cccgaaatcg ccaaaattgc ccggcttcaa tatgtcaccg attcgagcaa
      541 aggctttcga cgggtcgcgt ccgggaaggg tttcaaatat ctggacatgc agggaaaaac
      601 gatcacggag gacgccacgt tgcggcgcat caagggcctc gcaatcccgc cggcgtggaa
      661 aaatgtgtgg atttgcccgg ttgaaaacgg ccatttgcag gcgacgggct gtgacgaccg
      721 caagcgcaag caatatcggt atcatccgcg ctggcgcgag gtgaaggacg aaacgaaata
      781 tgccaagatg ctggcgttcg cgaaggcgct gccgaagatc cgcaagcgtg tcgcaaaaga
      841 tttgcgcgcg ccggagttgt ccaagcgcca ggtgctggcg acggtggtgc gtttgctcga
      901 agtgagcctc atccgcgtcg ggaacgaaga atacgcccat gaaaaccatt cttacggttt
      961 gacgacattg aagaaccatc acgccaaggt gcaaaaatcc accatccaat tccatttccg
     1021 cggcaagagc ggcaagctgc gtcaggtcga ggtgcacgac gcgcggctgg ccaggattgt
     1081 ccgcgaatgc cgcgacattc ccgggcagga tttgtttcag tacattgatg cggacggtca
     1141 gccgcagcaa atccgctcgg aagatgtgaa tgattatctg cacgaaattt ccggcggcga
     1201 tttcaccgcg aaagatttcc ggacctgggc cggaacagtt ttggcgggca tcgcattgca
     1261 ggaattccaa aaattcgaca ccaaggcgca ggcgaaaaag aatgttgtgc aggcgattga
     1321 atcggttgcg acgcgtctcg gcaacacgcc cgccatctgc aagaaatgtt acatccatcc
     1381 ggaaatcctc gaaggctact tgcaaggcgc aaccatccag accatccgcg cccgcgctga
     1441 aaccgagttg aagaaaggac ttcatcaact tcagcccgag gaagcggcgg tggtggcttt
     1501 tctgcaaaag cgcctcgccg agaaggaaca gccgttggaa aaaatactga aacgaagctt
     1561 gaaacgattc aaacatccgc acctgccggg gcaccaacgt tgatatggga tgattacccc
     1621 atgtattgcg gaacaaaaaa agcggtgcaa tggttctaca tagaaatatg aataagtctt
     1681 caaaaccccg ttctcagcat gaccggtttc ggcatttcgc ccatgcagcg gcgaattgga
     1741 tggggaatcc ttggtgcttt ttcttcgcgc tcgtcattat tgtggcgtgg ggcatcaccg
     1801 ggccgttgtt tcacttttcg gacacatggc aactgatcat caacaccggg acgacgatca
     1861 tcacgttttt gatggtgttc ctcatccaaa acacgcaaaa ccgtgacgcg caggcgatcc
     1921 atctcaaact cgatgaactc attcgcgcga tgcaaggcgc acgcaaccag atgttgaact
     1981 tggagcatct gtccgatgaa gaattggccg aattaaagcg ccagttcgat aagatttgca
     2041 aacgcgcgac gcgttcgtct ggagagttgg cggacaaatt gccgtgaaca atttgcaagc
     2101 caccaaatgc ttggcttgtt tttctccagc caatgagtag aatcaagtca cgcatgaaga
     2161 cgccctgatg ggcgatgtgc tcacgatgga aactcaaata ccgaaacgtc cggcagcgcg
     2221 gttcaaggga cggctgatgc tttgcaacat cggccgggtg ggcgacacca tccttcgcaa
     2281 ttccatcctc gattcgggtt ttcgcactta ttcgacggtg gattacatct gcgggccgaa
     2341 caatgtggag ttgatccgcg ccgatccgcg catcaaccgg atcacggtat tgcgcaattc
     2401 gatttccggg ttcgccagcc tgctcatgac ggcggcgaga gggcgttacg ataattttat
     2461 cgaactcaag gaccattgga gttcgacaag cgtcttgatc acgggcttct ttcgtagccg
     2521 cgttaaaacg ggttggaaca gcgatagatt caaaccgttt catcgcgatg tgcgcacggt
     2581 ctatgaaccg cagatgcaca agatagagat gatgcggcgg gttgcggagc ttgccgggct
     2641 cgaaccggga gaatataagg cgacgttggt gcttcctgcg gattcggtgc ggtggttcga
     2701 cgagaactac aaatggaaag agcctttcat ttttctgaac atctcggcga cttcggagga
     2761 ccggatgtgg ccgacgatga attgggcgcg ctatgtgcga agctgcggat tggaggggcc
     2821 gattctcgtg aatggcgttc caaaggaccg tgatgcagtg catcagcttt gcgcggaatt
     2881 gccgggttca atcgcgttca ggccgcgcca attcatggac gtcgccgcgg cattgaatcg
     2941 tgcgcgcctg gtgttgacgg tggacacagg cgtggtccac gcttgcagcg cgttgaaccg
     3001 gccaatcatt gcgctgggcc acagcggcaa ggaatacagc ccgctgtcaa cgcgcacatt
     3061 gatgattcgc acccgggact gcaccgtgcg ccaattcgat cccgaactgg cgattgcgga
     3121 aacattgcgg cacggtttgc cgtaaagctc agtctcgttt aagtaattgt tccagatgat
     3181 gaagcacgac aggaacagaa agttgctcaa gcccttttga atcacgcagc atggtcattt
     3241 cacgaccgcg cggacgccac actgcttcgt ctgtttcacc ccacaaaatc aaagaccgca
     3301 cgccgaccgc cgacgcgaga tgcgaaatcc ccgaatcatg gccaacgaaa ccgacgcacg
     3361 aggcgagcca ttgcgcaagt tcggaaagcg gcgcgccttt catgattttg atgcgtgtcg
     3421 aaggcagggc acgcgcgaga cggtccagcc gttcgcgttc cgcttcgccg ccgatcagga
     3481 gcaatttcag cttggtgttg ctcatcaatt gctgaagcaa ttcattccac ttcgcttcgg
     3541 gccagttttt attttcgctg ccgctgccag gatgcaccgc aagccagcgt cccgtggtga
     3601 gcgttgtttc aaacgccttg gtcacatcga gtcgcggaac ggcgtcggct tcaaagatgg
     3661 caagacgttc gagcggcttc aaaaaaactt cggtcgcgtg gacctcgtct ttttcgtcag
     3721 gtcgatgcgg cccgggaatg aattgcgcgc gcgtgcaacg gccgacgttc gttttgaaaa
     3781 tctcgtcagg atcgtaaagg taggaaatga tgagttgaaa cccggcgaaa tattccgcga
     3841 gttcctttgc cagttcgccg ttacgcgcaa aaaaacccgc caggggacgc gcctcgatgg
     3901 attgcacacg actggccaat ccaccggcaa gcgcgagttg cgcgatgtgc gggtagccga
     3961 gcacttcgag ttctgcgcgg ggaaattgct gccgcaacgc cgccagcacc ggcaaggtaa
     4021 ggatgaaatc gccgatcgcg ccgccgcgaa tcacgagaat tttggcttga gtggccggca
     4081 tgtttacgga ttgaaaattt tgctggaaac ggaagctcca aattccaagc tccaactcgc
     4141 aagcttcaaa accagacgct cggcctttgg tgcttggtta ttctctggat gttggaggtt
     4201 ggcatttgga gctttgtctt tcatgcactt cgatacaccg taaatcccag cactgccacg
     4261 aacaacccga acgccaaccg gatggcgctg ccgatgcgaa cgcgcttttc attggcggtc
     4321 gcccagttca acaaatcgcg caaatgccat ggataaagcg tgaaccagat tccgccaatg
     4381 accagcacat aggcccacgt gacaatcaca aggttccatt cagtatttgc ccagcgcgca
     4441 gtgtccacca ttagcttggc gagcaatagc atcaccaccg cgagcccgcg caccgcgaga
     4501 aaatcctgca cataaacgca cgatgcgatg cccaccgctg caaatccgcc gagcaacacc
     4561 ggcttgtaag cgacgaaatc cgagagcgat tctgcattga cataaaagac gaaccacgct
     4621 gttcccagca gcatcaaggt gattccccac ggcagcgagc gcggaaattt ccgcacgccc
     4681 gccgcaaatg cggctggttt catcagcccg taaatctgcg gcaaacccac gagcagaccc
     4741 aaaattatag ccaatgtcga aagttgaacg tgcatatttc gcccacaaac taaacgaaaa
     4801 taaaaaatcg gaaagccatt ttctttgacc attcttcggg cgcgccacta tcatccgcca
     4861 catggcttgt cgaatgtttg gatattgcgg aactctggaa tcggacgtgc gcgaattgta
     4921 ttccggcctc aagcgcgcct gcgccaacga tcctgccttg caatccgtcg cgccgaattg
     4981 ttcgcaacat tcgcatgggt ggggctatgt cattcacgcc gacaacggac ttttccatta
     5041 ccgcacttcg cgctcgattt atgaggacga catcgtcctg ccaaaattgg agggccaaat
     5101 ccgcgcgatt ttccacggac gctacgcgag caacctggaa ttgatgggcc acattttttc
     5161 gcatcccttc atggcgttca atgacaagca gacaattttt ttggcgcaca atggcggcgt
     5221 tgacccgaag gaaaaaaaaa tttccgagcg aaaagtggac agcgaatggg cgttggaaca
     5281 aatcataaaa gcgggcgacg tcgcgtccgc gttgccgaaa cttaaggagc acaccaagtc
     5341 cgcgctgaat ttgttgattc tcacgattga ccgcgaaaaa ggaactcccg cgctgctgca
     5401 atatttccat tacttcaagc ccaaggaacc ggagaaggtt aagtattata aaatgtatga
     5461 aggcgcgatg cccggcggac gcgctttggt ttcgtcaact atgacttttt ccgaagccaa
     5521 aatgggaagt ttgacgaata ttgagcctgc taaatttgat gagctgatga agttgggata
     5581 gagagaagcg ccaatcatca acatccaagc tccatagaat aatcaatgac caagcttcga
     5641 ctcgcaacgg gttagcgcat caccaattgg agcttgaact ttggagcttc tctggagttt
     5701 ggatgttgga gcttaaacat cactccagaa tcgccggatc gccatacaac gtaaacgaac
     5761 caacccgtgt tatttttacg tccattactt gcccgatatg ccgtgacgaa ccgtcgaaca
     5821 ccacaatctt attgcaacgc gtccggcccg tcaggcgcgc gggatttttt ctgctcgggc
     5881 cttcgaccaa aatttccacg ttgccgccca cataagcaac gtaatgccgc gcgccgattt
     5941 cattgatgag cgccagcaat cgcgcgttgc gctcttcttt gaccgcttgc agcaattggt
     6001 cagaaagcgt ggccgctggc gtgtctttgc gcggcgaata tttgaagatg tacgcgttgt
     6061 cgaactccac ctcgcgcgca agcgagagcg tctcttcaaa atccgcttcc gtctcgccgg
     6121 gaaatccgac aatgatgtcc gtcgtgatgc ccatgcgcgg gcgcacatgg cgcaactttt
     6181 tgatgatgcc catgtatctc tcacgcgtgt aaccgcgatg catcgccttc aacagccggt
     6241 cgctgccgct ttgcaccggc aaatgcgcgc tctcgaccag cttgggcaaa cgcccatacg
     6301 cctcgactaa atcatcgcca taacctttcg gatgcggcga agtaaaacga atccgttcca
     6361 atccctcaat ttcatgcacc gcttcgagca attgcacgaa cgccgacttg ccgtccctca
     6421 ccggaatgtc gcgcttgccg taactcgtca cgatctggcc gagcaaagtg atttccttca
     6481 cgccgcgcgc gaccaattcg cggcattcgg ccacgatgcc ttcaatactt cggctgcgct
     6541 cctcgccgcg cgtgtatggg acgatgcaaa acgtgcaata ttggttgcac ccttgcatga
     6601 tgctgacgaa tgcgctcact gatttcgccg cgctcttgcc attcaaaatg tgttccttga
     6661 tggccgcttc gctcccctgc tcttcagcgg tgtccacaat tttttcgcgc acgccggaaa
     6721 aaatttcgtc gagataatcc gctgtgcgat gaaatttttg cgtgccgatg accaggtcaa
     6781 cgtccggcaa ttgatccacc aactgttgtc cgcggctttg cgccatgcaa cccatgaaac
     6841 caagcacgac gttggggcgg ttcttgcgca cttcggcggc gagattttgc atcttgccga
     6901 tggccttttg ctcggcggca tcgcgcacgc tgcaagtatt gaggagcacg acatccgcga
     6961 ccgtttcaga cgacgcgaga gtataaccct tcgcaacgag ttgcgcggcg accgcctccg
     7021 aatcgcgctc gttcatctgg catccgtacg ttttgatata gacactcggc atgatgctga
     7081 gaatgcaatg aaattaaggc agaaaaagga acttggaaag aaggaaaccg aaagaactca
     7141 ttcaacttaa cttccaagcc gcgatgattc aatcctgatc caaggaggcg cgcggaattt
     7201 tatctgtcct ttgattttgc ggacggcaag gacgatagct ttatccgctg gtaagcaccc
     7261 cttttttgat cctctggatg cggctcaatg gccaacaccg ttatgtctcg tttatccggc
     7321 ccgtaagccc aaaaaatcct tccggctgcc ggcgtattgt tttcgagata tgattgaaaa
     7381 attttgaggc cgtatttgct ggttaaatcc tcaatctcat gggaagcgag gctgttgtac
     7441 cgcgggtttt cggcgagata gccaagcgct ttgaccagtt ttttgaaaaa tttctcctcg
     7501 ttcttgtcca atttgccctg ctgcttgcgc gccgaaaggt cattccaaaa ggattccatt
     7561 tccggaactc ccatgcggat attaaatgcc attcgccctc ttaatcctca ttaaaggcag
     7621 ccaggtttat tggagcggaa gccatcccct ttttcagatt ggcgacggat ttatccagca
     7681 ttttcaggga acgatctgaa acggcttgcg gcggcaccaa tacgcggggt tcgagaagat
     7741 aacagccatt cgacagcgcc ttgacgtgaa aatatttcgt cttggcattg cgcaggctga
     7801 ttcgtttttt tgcatcagcc gcgacgtcgt aattttcaat caccttgagg gcctgctttt
     7861 gtgtcattgt cggcatgatt taaatatgtg ggatttccca cctattgtca at
//
LOCUS       DASZMM010000567         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1891918, whole genome shotgun sequence.
ACCESSION   DASZMM010000567 DASZMM010000000
VERSION     DASZMM010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891918"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VG754_13645"
     CDS             <1..203
                     /locus_tag="VG754_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF20308.1"
                     /translation="NPGMIETEGVHSAGFINTDFQKGVEAQSPLGRIGQTDDISPTAV
                     YLASSDSKYVTGETLLVSGGIR"
     gene            351..2189
                     /gene="rpoD"
                     /locus_tag="VG754_13650"
     CDS             351..2189
                     /gene="rpoD"
                     /locus_tag="VG754_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWF20309.1"
                     /translation="MTDGQSVETLAGCPLSQWGWQTNMSKSNKSVDRAAAKLTGKNSN
                     KTASAAAAAANAGAQLHAGSITSKSGVDLTEKIRELLVLAKEQGHLTHDDINEALPDT
                     VVGPADLDQIYAKLNSLEVEIVEQAEVDRVKQPEPEEEEEKGRLDILDDPVRMYLRQM
                     GKVPLLTRDQEVAICKRMEAAENEQKKIIYGFGFTAKEHIALAEKLISEPPKERFDRV
                     IVDSKVESRERHLKILRALVKKTRELDQKVDEKYAAWQAGAAASREKLFNEFLKLDKK
                     LQTAFPKFHYKQKVIEEMVMVTQNIHDKIHASLRAIASVETQRKSEQQQSILKSEKHK
                     IKALEEFARMPYEEYLKAHKELNACAARTDQARNEMAEANLRLVISIGKKYTNRGLPF
                     LDLIQEGNIGLMRGVEKFEYRRGYKFSTYATWWIRQGITRAIADQARTIRIPVHMIEI
                     IGKLMRAQKELFQEFGREATPEEIADEMQLSSERVRSILKMAQNPISMQSTVGDSDDA
                     CFGDFIEDKAAENPSLMTANSLLKEKLQDVLATLNERERRIVEMRFGLADGYGRTLEE
                     LGKHYNVTRERIRQIEAKALRKLRHPTRSLHLKGFLEVAEEVDVAA"
     gene            complement(2279..3019)
                     /locus_tag="VG754_13655"
     CDS             complement(2279..3019)
                     /locus_tag="VG754_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWF20310.1"
                     /translation="MKNTISNETRTEKPVITKLAAGARGKTEIGWLHSRHSFSFGEYF
                     DPDQMGFRSLRVINDDIVEAGQGFGTHAHRDAEIFSYVIEGELEHKDSLGNGSVIKAG
                     DLQYISAGSGVRHSEFNPSKTNRVHFLQVWLMPNVTGGQPRYAEKTLGDAAKANALTL
                     LFAGKPADGVVQIRQDAEIYFGKLDQGTTLPVELGADRHAWVHVIKGDVQALAETLKE
                     GDGLAVSNVSRFDIVGQENSQFLLFNLA"
     gene            3107..>3948
                     /locus_tag="VG754_13660"
     CDS             3107..>3948
                     /locus_tag="VG754_13660"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWF20311.1"
                     /translation="MELRHLRYFATVAEEKTFSRASARLRISQPAVSRQIKDLEEELG
                     VSLFIRHGPTVSLTPAGEAFLAHARDVLRRAARAAEEMKTFQKNTAEKFSIGYIAPVL
                     AGTLTPALRCFEQKHPQVELNLVELTPPDQIEALRSGRIDVAFIGNACPALQSEFNLA
                     VLQEIQLQAVLPDNHHLALRKELKLTELADEPFVGFSERALPGRNDTICGACQEAGFT
                     PAIRFQAESLSAALALIAAGKGVTLMPEEVSRLSHLQTVFVPLNGLAPYITSVAASAK
                     GNTR"
BASE COUNT          886 a         1217 c         1082 g          763 t
ORIGIN      
        1 tcaatcccgg gatgatcgag accgaaggag tccactccgc gggtttcatc aacactgact
       61 tccagaaggg agttgaggct cagagcccgc tcggacgcat cggccagacc gatgacatct
      121 ccccgaccgc cgtctatctc gcctcctcgg attccaaata cgtgacgggt gaaaccttgc
      181 tcgtctccgg cggtatccgc tgatttggga agcggccgga tgccatgctc acagcggagc
      241 cagccccccg aggcgctcca ctgatgggca tggtatcagg ctggctcaac gcagaggtcc
      301 gttcttcggc cggtcccacc cgtacagcat gagatttatt gagcaagtgt ttgacggatg
      361 ggcaatcagt tgagacactc gctggctgtc ctctatcaca atggggatgg caaacgaata
      421 tgtctaaatc caataagtct gttgaccgag ccgcagccaa gctcactggc aagaattcca
      481 ataaaaccgc ttctgctgcc gcagcggctg caaatgccgg cgcccaactg catgccggtt
      541 ccatcacctc caagtccggc gtggacctga cggaaaaaat ccgtgaattg cttgtcctcg
      601 ccaaggagca gggacatctg acccatgacg atattaatga agcgctgccg gacaccgtgg
      661 tcggcccggc cgaccttgac cagatttatg ccaagctgaa cagccttgaa gttgagatcg
      721 tggagcaggc cgaggtggac cgggtcaaac agccggaacc agaggaggaa gaggaaaaag
      781 ggcgcctgga cattctggat gatcccgtcc gcatgtatct gcggcaaatg gggaaggtcc
      841 cgctgctcac gcgcgatcag gaggtggcca tttgcaagcg gatggaggcg gccgagaacg
      901 agcagaagaa aattatttac ggctttggtt tcacggccaa ggagcacatt gccctggcgg
      961 aaaaattgat ttccgagccg cccaaggaac gatttgaccg ggtgatcgtg gatagcaagg
     1021 tcgagagccg ggaacggcat ttgaaaattt tgcgcgccct cgtcaagaaa acccgcgaac
     1081 tggaccagaa agtggacgag aaatacgccg cgtggcaggc tggggccgcg gcttcccggg
     1141 aaaaactgtt caacgaattc ctcaagctcg ataaaaagct ccagacggcc tttccgaaat
     1201 ttcactacaa acaaaaggtc atcgaggaaa tggtgatggt gacccagaac attcatgaca
     1261 agattcatgc cagcctgcgt gccatcgcca gcgtggaaac ccagcgcaaa tccgagcagc
     1321 agcaatcgat cctcaaatcg gagaagcaca agatcaaggc gctggaggaa tttgcgcgca
     1381 tgccgtacga ggaatacctc aaggcccaca aggaactgaa cgcctgcgcc gccagaaccg
     1441 atcaggcccg aaatgaaatg gccgaggcca acctgcgcct cgttatttcc atcggcaaga
     1501 aatataccaa tcgcggcctc ccattcctgg acctcatcca ggaaggcaac attggactga
     1561 tgcgcggcgt ggagaaattc gaatatcgcc gcggctacaa gttttccacc tacgcgacct
     1621 ggtggattcg ccaggggatc acccgcgcca tcgccgacca ggcccgcacc atccgcatcc
     1681 cggtgcatat gatcgaaatc atcggcaagt tgatgcgcgc gcagaaggag ctcttccagg
     1741 aattcggccg cgaggccacg ccggaggaaa tcgccgacga aatgcagctt tccagcgagc
     1801 gcgtccgctc cattctcaaa atggcccaga atcccatctc catgcagtcc accgtcggcg
     1861 acagcgacga cgcctgcttc ggcgatttca ttgaggataa ggcggcggag aatccgtcac
     1921 tcatgacggc gaacagcctc ttgaaagaaa aactgcagga cgttctggcc accctgaacg
     1981 aacgcgaacg ccggatcgtg gaaatgcggt tcgggctggc ggacggctac ggtcgcacgt
     2041 tggaggagtt gggcaaacac tacaacgtca cccgcgaacg catccgccaa atcgaagcca
     2101 aagccctgcg caaattgcgc caccccactc gttccctcca cctcaaaggc tttctcgaag
     2161 tggcggaaga agtggatgtg gcagcttgaa ccggcgccaa cggtagtctt tcctcaatgt
     2221 aaaatgcgcg gcggaaattc cgccgcgcat tttggctttt gcccggttcc cgaatttctc
     2281 aggcaaggtt aaacaggagg aattgcgagt tctcctggcc gacaatgtca aagcgggaca
     2341 cattcgacac cgccaggccg tcgccttcct tcaaggtctc cgcaagagcc tgcacatcgc
     2401 ccttgatgac atgcacccag gcgtgacgat ccgcgcccag ctccaccggc agcgtggtcc
     2461 cctggtccaa cttgccgaag taaatttcgg cgtcctggcg gatttgcacc actccatcgg
     2521 cgggcttgcc cgcgaataac agcgtcaagg cattggcttt ggccgcatca ccgagggtct
     2581 tctccgcata acgcggctgg cccccggtca cgttgggcat gagccagact tgcagaaaat
     2641 gcacgcgatt cgtcttcgac ggattaaact cgctgtgccg cactccgctg cccgcgctga
     2701 tgtattgcaa gtcgccggcc ttgatgaccg agccgttgcc caggctgtcc ttgtgctcca
     2761 attcgccttc gatgacgtag ctgaaaatct ccgcatcgcg gtgagcgtgg gtgccaaacc
     2821 cctggcccgc ttccacgatg tcatcattga tgacgcgcaa ggagcggaac cccatttggt
     2881 ccgggtcaaa atattcgccg aatgaaaaag aatggcggct gtgcagccat ccgatttccg
     2941 tctttccgcg cgctcccgcc gccagcttcg tgatcaccgg cttttccgtt ctggtttcat
     3001 tcgatatcgt atttttcata tgcctacagc ttcggctttg gaatgaaggg cggccaatgc
     3061 ttttttgttt ggccggcccg gacttttggt tataggttac gggcgcatgg aactccgcca
     3121 tctgcggtat ttcgccaccg tcgccgaaga aaaaaccttc agccgcgcct ccgcccgatt
     3181 gcgcatttcc cagccggccg tgagccgcca gatcaaggat ctcgaggagg aactcggcgt
     3241 gagcctcttc atccgccatg gcccgaccgt gagcctcacc ccggcgggcg aagctttcct
     3301 ggcccacgct cgcgacgtcc tccgccgcgc ggccagagcc gccgaggaaa tgaaaacgtt
     3361 tcagaaaaat actgccgaga aattttccat cggctatatc gctcccgtcc tcgcgggcac
     3421 cttgaccccc gctttgcgct gcttcgagca aaagcatccc caggtcgaac tgaatctggt
     3481 cgaactgacg ccgccggatc agattgaagc gttgcgctcc ggacggatcg atgtcgcctt
     3541 catcggcaac gcctgccccg cgctccaatc agaattcaac ctcgctgttc tccaggaaat
     3601 tcaactccaa gccgtcctgc ccgacaatca tcacctcgcg ctgcgcaagg aactcaaatt
     3661 gaccgaactg gctgacgaac cgttcgtggg cttctcggag cgcgcgctcc ccggccgcaa
     3721 cgacaccatt tgcggcgcct gccaggaagc cggcttcacc ccggccattc gttttcaggc
     3781 cgagagtctt tccgccgcac tggccctcat cgcggcgggc aaaggcgtga ccctcatgcc
     3841 tgaggaagtg agccggttat ctcatcttca aaccgtgttc gtccctttga acgggctggc
     3901 cccctacatc acttccgtgg ccgcatcagc caaaggaaac actcgcag
//
LOCUS       DASZMM010000568         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4651551, whole genome shotgun sequence.
ACCESSION   DASZMM010000568 DASZMM010000000
VERSION     DASZMM010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4651551"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2223
                     /locus_tag="VG754_13665"
     CDS             <1..2223
                     /locus_tag="VG754_13665"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HWF20312.1"
                     /translation="VTSLVAKGTNVIGAIVADGWYSGYVGFGRLLGLGPNRVGRYLYG
                     KTPALLAQLEIEYTDGSRESIFTDPTWKVTGEGPIREADLIMGESYDARLELNGWSAT
                     GFNDAKWENAIRAEYNGSTKADFFDNSGDREVELGFQVPAKLQAYPAPPVRVTEELTA
                     QHLSEPQPGVYIFDMGQNFAGVIRLHVKGTAGTKVQIRYGEHLYPDGPLMTENLRKAR
                     ATDYYTLRGDASGETWTPRFTYHGFQYVELTGLPEKPDLDAVTGLVLQSDTPMIGSFS
                     CSDDVLTRFGQNARWTQRANFMDIPTDCPQRDERLGWMGDAQTYVRTATFNADVAAFY
                     TKWLDDVEEAQRDFGAYPDYCPYPMSPGPENKAFGAAWTDAGVICPWTIWKVYGDTRV
                     IDRHWASLTRFMDWRAASTTHDGLGISIGNGWGDWLNVNEPTPIEYIDTCYHALDCKL
                     MADMANATDRKFEFTNYLSQFDKIKSAFNKAYVDTNGTLKVDSQTAYVLALWTGLLPE
                     DLVGQSVTNLAEKIARNGYRMTTGFLGTRALLPVLSANGQHDLAVRLFQSRQFPSWGY
                     EVVNGANTVWERWDSFTQEHGFEGANGKQNASMNSFSHYSFGAVMEWAYRDLAGIDTD
                     GAGYQRIFIKPGPPSRDSDPYITPVNWVKADYVSVRGRIVVDWNKEPERFVLNVTIPA
                     NTTASVFIPAKSIADIYESGHPLNQSQGVRYLRMEKDYVVVRVESGQYNFVTQPPPGS
                     "
     gene            2399..3238
                     /locus_tag="VG754_13670"
     CDS             2399..3238
                     /locus_tag="VG754_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWF20313.1"
                     /translation="MDIKKAIITAAGKNQPTLPLQTLVDRDGTQKTALTIILEEVLKA
                     GIQEICVVVRPGDQSAYRGAAGAHAGRVQFIEQTKPLGYGQAIQCAREFAGNQPFLHL
                     VGDHLYVTRDLKNCAQQLVEVAQAESCAVSAVQATHESKLPYFGAVGGRLVAGRQRLY
                     EIENVLEKPAPTEAEQKLIVPGLRAGRYLCFFGMHVLTPTVMELLNEMVAAATGNVTL
                     TDALNRLGARERYLALELRGRRYDIGVRYGLLTAQLALAFEGKDRDEVLSNLVEMLAT
                     KPE"
BASE COUNT          740 a          963 c          960 g          710 t
ORIGIN      
        1 gtgacttcat tggtcgcgaa gggcacgaat gtcatcggcg caatcgtggc ggatggctgg
       61 tattcgggtt atgtggggtt tgggcgtttg ttggggcttg gtccgaacag ggtggggcgt
      121 tatctctacg gcaaaactcc cgcgctgctt gcgcaacttg aaatcgaata caccgatggc
      181 tcgcgcgaaa gcattttcac tgacccgacc tggaaggtca ccggtgaagg gccaattcgc
      241 gaagccgacc ttatcatggg cgaaagttat gacgcgcgcc tggaactgaa cggttggtct
      301 gcaactggtt ttaacgacgc caaatgggag aacgccattc gcgcggaata caacggcagc
      361 acgaaagcgg atttttttga caacagcggt gatcgcgaag ttgaacttgg ttttcaagtg
      421 cctgccaaat tgcaggcgta tccagcgccg ccggtccgcg tcacggaaga attgaccgcg
      481 caacatctct cggaaccgca accgggcgtt tacatttttg acatgggaca aaactttgcg
      541 ggcgtcatca ggcttcacgt caaagggact gcgggcacaa aagtgcagat ccgttacggc
      601 gaacatttgt atcctgacgg gcctttgatg accgaaaacc tgcgcaaggc gcgcgcgacc
      661 gattattata ctttgcgagg tgatgcgtcc ggcgaaacat ggacgccgcg tttcacttat
      721 cacggatttc aatacgttga attgaccggc ctgccggaaa agccggacct ggacgccgtc
      781 accggactcg tgttgcagag cgacacgccg atgatcggct cgttctcctg ttccgacgat
      841 gtgctgactc ggttcgggca aaatgcccgt tggacgcagc gcgccaattt catggacatc
      901 ccgaccgatt gtccgcaacg cgacgaacgc ctcggctgga tgggtgatgc gcaaacttac
      961 gtccgcaccg ccacgttcaa tgcggacgtc gccgcgtttt acaccaaatg gctcgatgac
     1021 gttgaggaag cgcagcgcga ttttggcgct tacccggatt attgtccgta cccgatgagt
     1081 cccggcccgg agaacaaagc tttcggcgcg gcttggaccg acgcaggcgt catctgcccc
     1141 tggaccatct ggaaagtcta tggcgacacg cgagtgattg accggcattg ggcttcgctg
     1201 acgcggttca tggactggcg cgccgcaagc acgacgcatg acggcctcgg catcagcatc
     1261 gggaacgggt ggggcgactg gctcaatgtg aacgaaccga cgccgattga atacattgac
     1321 acctgttacc acgcgcttga ttgcaaactc atggccgaca tggcgaacgc gaccgatcgc
     1381 aaattcgaat tcaccaatta tctttcccaa ttcgataaaa tcaaatcagc gttcaacaag
     1441 gcttatgttg ataccaacgg cacgctcaag gtggactcgc aaacggctta tgtcctcgcg
     1501 ctttggaccg gattgttgcc ggaagatttg gtcggacaat ccgtcacaaa cctcgccgaa
     1561 aaaatcgccc gcaatggtta tcgcatgacg actggttttc tcggaacgcg cgcgttgctg
     1621 ccggtgctca gcgccaacgg ccagcacgat ctggcggtgc ggttgtttca gagccggcaa
     1681 tttccatcct ggggctatga agtggtcaac ggcgcgaaca ccgtttggga acgatgggac
     1741 agtttcacgc aggagcacgg cttcgaaggc gcgaacggaa agcaaaacgc ctcgatgaac
     1801 agtttcagcc attattcgtt cggcgctgtc atggaatggg cctaccgcga tctggccggc
     1861 attgatactg atggcgcggg ttatcaacgc attttcatca agcccggccc gccgtcccgc
     1921 gacagcgacc cttacatcac gccggtgaat tgggtgaagg ccgattacgt cagcgtgcga
     1981 ggccgcatcg tcgttgattg gaacaaggaa ccggagcggt tcgttctcaa cgtcaccatt
     2041 cccgccaata ccaccgcgtc agtatttatt cccgcaaaat ccatcgccga catttacgag
     2101 agcggtcatc cgctgaacca gtcccaaggc gtaagatatt tgcgcatgga aaaagactat
     2161 gtcgtcgtgc gcgtcgaatc cgggcaatac aatttcgtga cgcaaccgcc gccagggtcg
     2221 tgatccggcc agcgctactt ggcgcaggct ttccagcctg cggattgcgg ggactttcca
     2281 gtccccgctc cagaacagtg tccagatgcg cacatccatg actgaagctt atgactctca
     2341 ttgaccatta ttgccgaatc tgcaacgctt tgcgtggtag aataaattaa ttttttccgt
     2401 ggacatcaaa aaagccatca tcaccgctgc cggcaaaaac cagcctacgc tgccgctcca
     2461 gactttggtt gaccgcgatg gcacgcaaaa aaccgcgctc accatcatcc ttgaagaagt
     2521 cctcaaggcc ggcatccagg agatttgtgt ggtggttcgc ccgggagacc agtcggctta
     2581 tcgcggcgcg gcaggcgctc atgccggacg ggtccaattc atcgagcaaa caaaaccgct
     2641 cggttacggc caagccatcc aatgcgcgcg cgaatttgcc ggcaaccagc cgtttttgca
     2701 tctcgtcggc gaccatttgt atgtgactcg cgacctgaaa aattgcgcgc aacaactcgt
     2761 cgaagtcgcg caggctgaat catgcgccgt ttccgccgtg caagcgacgc atgaaagcaa
     2821 gctgccttac tttggcgcag tgggcggaag gctggttgcg ggacgccagc ggctttatga
     2881 gattgaaaat gtcctggaaa aaccggcccc caccgaggcg gaacaaaaat tgatcgtgcc
     2941 cggtttgcgc gccggccgtt atctctgctt tttcgggatg catgtgctga cgccgacggt
     3001 gatggagttg ctcaacgaaa tggtcgccgc tgccaccggc aacgtcactt tgacggacgc
     3061 gctcaatcgt ctcggcgcgc gtgagcggta tcttgcgctc gaattgcgcg gacgacgcta
     3121 tgacatcggt gttcgttacg gacttctcac ggcgcaactg gcgctggcct tcgaaggcaa
     3181 ggaccgcgac gaagtgctgt ccaaccttgt cgaaatgctc gcgaccaagc ccgaatgatt
     3241 ttcccatgac cggcaggctt cgccacatca tcaccgcgtc cgatccggcg gcgcgcaatc
     3301 aatcgctcga tgcctttgcc cgcgccgcat cgcttgccga attgctggcc gagtgcgaag
     3361 acctcgaagc gtt
//
LOCUS       DASZMM010000569         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4109327, whole genome shotgun sequence.
ACCESSION   DASZMM010000569 DASZMM010000000
VERSION     DASZMM010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4109327"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VG754_13675"
     CDS             <1..929
                     /locus_tag="VG754_13675"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWF20314.1"
                     /translation="AVGQVFNEPGSLFRTLVKALRPHQWVKNIIIFVPVLTSHNLGNP
                     RFLAQATLAFISFSFCASGVYVLNDLLDLAADRHHPTKKNRAFASGRLPLPVGFLLSP
                     LMLGLSAAIAVFLSWHFLIVLAAYLALTTTYSWHVKKIPLLDVFFLAGLYTMRLIAGH
                     AATGIPKSFWLFAFSMFIFLSLALVKRYTELHSLRRENRQDTRGRGYTSGDLEIVAML
                     GIASGFMAVLVMALYVNSEEVRLLYGHQRRLLLICPILLYWLSRVWLLAHRGKMHDDP
                     IVFALKDSVSYLAGALTLGVVWLATGHYKWFF"
     gene            961..>2100
                     /locus_tag="VG754_13680"
     CDS             961..>2100
                     /locus_tag="VG754_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWF20315.1"
                     /translation="MNRLLQSWFHDTVSALAYSAAQTRADASAPDLQTPYNDLTQFVL
                     QQHARMAGYLRTPLMAATLGFDLFGFFHGGKRFHKLRPELRACQMEAWRNSSVGFQRD
                     LMRYFDSLAMLELHSRSTSGQCTPAQSGAVSSNQVISTPASVSRYEIVVIGSGPGGAI
                     TACLLAEAGRKVLLMEEGDYLPLESCAPFSKDEMVQKYRNGGQTVAMGANKVAYVEGR
                     CVGGGSEINSGLYHRTPPETLEAWRKEFRVEGLDELRPHFEACEKEVSVSFLPGAAPP
                     ASLKLHEGAIKLGWKSLEVPRWFSYASGTTDATDGKRQSMTETFIPRFLKAGGQLLPR
                     TRASSIRQDGNHWFINAHHAITGPLLISAENLFLCAGAVQTPALLR"
BASE COUNT          407 a          564 c          602 g          527 t
ORIGIN      
        1 cggcggtggg acaggtgttc aacgagccgg gttcgctttt tcggacgttg gtgaaggcgt
       61 tgcgaccgca tcaatgggtc aagaacatca tcatttttgt gccggtgctg acttcgcaca
      121 atcttggcaa tccgcgcttt cttgcgcagg ccacgctggc gttcatctcg ttcagttttt
      181 gcgcgtcggg cgtttatgtg ctgaacgatc tcttggacct cgccgcggac cgccatcatc
      241 ctacaaaaaa aaatcgcgcg ttcgcttcgg gccgcctgcc gttgccggtt ggttttttat
      301 tgtcgccgct gatgcttgga ttgagcgcag cgattgcggt atttttgtca tggcattttt
      361 tgatcgtgct ggctgcatat ttggctttga cgacgaccta ttcgtggcat gtcaaaaaaa
      421 ttccattgct ggatgtgttt tttcttgccg gactttacac gatgcggctg attgcgggcc
      481 acgccgccac cggcatccca aaatctttct ggttgttcgc gttttcaatg ttcatctttc
      541 tcagcctcgc gttggtcaag cgttataccg aattgcacag tttgcgccgt gaaaatcggc
      601 aggacaccag gggacgcggt tatacaagcg gcgaccttga aatcgtggcg atgctcggga
      661 ttgccagcgg gttcatggct gtgctcgtga tggcgcttta tgtgaacagc gaggaagtgc
      721 ggcttctgta cggacaccag agaaggctgc tgttgatctg cccgattttg ctttattggc
      781 tgagccgcgt ctggttgctg gcgcatcgcg gaaaaatgca tgacgacccc atcgtcttcg
      841 cgctcaagga ctctgtgagt tatctggctg gggcgctcac gcttggcgtg gtttggctgg
      901 caacgggcca ttataaatgg tttttttagc ttcatgccgc gtttccaccg caacttctga
      961 atgaaccggc ttctccaatc ctggttccat gacaccgttt cggcgcttgc ctattccgcg
     1021 gcgcaaactc gcgcggacgc ttcggcgccg gatttgcaaa cgccttacaa cgacctgact
     1081 caattcgtgc ttcaacaaca cgcgcgcatg gccggttatc tgcgcactcc gttgatggcc
     1141 gcgacgcttg ggtttgacct gttcggtttt tttcacggtg gaaaacggtt tcacaagctc
     1201 aggcccgaac ttcgcgcctg ccagatggaa gcatggagaa attccagcgt cggctttcag
     1261 cgcgatttga tgcgttactt cgacagcctc gccatgctcg aattgcacag ccgctccaca
     1321 agcgggcaat gcacgccagc acaatccggc gcagtgtctt caaaccaggt gatttccacg
     1381 ccggcgtctg taagccgata cgaaattgtg gtcatcgggt caggcccggg cggagccatc
     1441 accgcttgtt tgcttgccga agccggaagg aaggttttgc tgatggaaga aggcgattat
     1501 cttccgctcg aatcctgcgc gcctttcagc aaggacgaga tggtgcagaa atatcgcaat
     1561 ggcgggcaga ccgtcgcaat gggcgcgaac aaagtcgcgt acgtggaagg ccgttgcgtc
     1621 ggcggcggca gtgaaatcaa cagcggcctt tatcatcgca cgccgccgga gacccttgaa
     1681 gcctggcgca aggaatttcg cgttgaagga cttgatgaac tgcgcccgca ttttgaagcc
     1741 tgcgaaaagg aagtgtcggt ttcgtttttg cccggtgcgg cgcctccggc atccttgaag
     1801 ttgcacgaag gcgcgataaa gctcggctgg aaatccctgg aagtgccgcg ctggttcagt
     1861 tatgcgagcg gtactactga tgcgacggat ggaaaacgcc aatccatgac ggaaactttt
     1921 atccctcggt ttttgaaagc gggtggacag ctcctgccac gcacgcgggc aagcagcatt
     1981 cggcaggatg gcaaccattg gttcattaat gcgcatcatg caatcaccgg acctttgctc
     2041 atctccgccg aaaatttatt cctttgcgcg ggcgcggtgc aaacacccgc attgttgcga
//
LOCUS       DASZMM010000570         7884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5200788, whole genome shotgun sequence.
ACCESSION   DASZMM010000570 DASZMM010000000
VERSION     DASZMM010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5200788"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VG754_13685"
     CDS             complement(<1..742)
                     /locus_tag="VG754_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HWF20316.1"
                     /translation="MSCNLPRIALLIESSRIYGRGILRGIAAYTHLHGPWSCFTEERE
                     LHSGIPDWLKEWKGHGIIARIEDKRAANSLLRLGFPVVDVLGNCQFKGIPGFDTDARE
                     VARLAADFFIRAGFRHFAFCGYWNIPFSDRRAAAFASCLRTQGYKLRVFPPVSGSVET
                     DDGLSRLPSDIQAVERRGLGRERAIAAWLRKQPRPLALFACNDVCGQQVLNACREHDI
                     KVPDEVAVLGVDNDDVLCNLCEPPLSSIE"
     gene            1093..4770
                     /locus_tag="VG754_13690"
     CDS             1093..4770
                     /locus_tag="VG754_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HWF20317.1"
                     /translation="MAHAGVGATTPFISFEAESGFLGGGATMVSVTSAPTNQYSSPEL
                     EASGHAYVQLTATNQYVQWTNNTGQSLTAINLRSCIPDAPAGGGITSTIDLYVNGVFR
                     QAFAVNSQQNYCYEGTNYNGQTDKNPADGDPRGFWNDTHAFITGAPIAPGDTFRFQMD
                     TANTAAFYDIDVVDLETPPSPLVQPSNSLSILSYEAVSNNIGVDNTSAINNCFTAARS
                     QGKSAWIPAGTFYISAIQGGLNAAGITIVGAGPWYSTINRVTPANNSQGVNNIITTVS
                     CTLSNVSLDCNGSSRAGNNNNGAVNFSGTNWVVDNVWIQHVTSAFWCAGVGGTARNCR
                     VLSVWSDGGNFNNVQSDDGIGMNLTYSNNFVRGTGDDAMAINSVNYNVFGSTTYYYTM
                     MSNITYVNNTAIAPWGGKCIGVYGGVNDLVTNNLLCDTARYIGLGIMKFGVNGSDLLS
                     ATVTGNTVLRCGGNGYNQQQQALMIGNGGDGQGVGTVANVYCASNTIINAMYDAIGFS
                     TSTNILFQRNTIINPALDAIAIGPPDLGSSVMGNALIYSNSVSGLKTGQVVFTNSSVG
                     YAGIIPTAATSYNNMSGVAPENCSEGGQDIGNITAGDWVAYNNVNLGGINAFVVRIAS
                     TNSGAGIQVRLDGANGTLIGNCVVPITGGSQTYANAYCSVSNASGTHTVYLVFSGGNG
                     NLCSVEFFALYYAPPDLSHQLVAGDVYSLNAVGNGKYVTEDSSYTNVLYAEGTTVSAP
                     QEFQIVDAGGGNIALQSLANDDYVSADNNGNSPLVANRTGVGLWETFTEVDAGNGNIA
                     LRAMNNGKYVTVDSLNGNVLIAKATTVGPQESFTVAFVSGVAPSAPAGLTASPGNSQV
                     VLSWTASPGATAYNIRRSTTNNGPYGIVASNITTLTYTNFNLTNGITYYYVVSAFNPA
                     GESTNSTQVTAIPGALDRSGWLASASSTESGGSPSYAIDGNVSTRWSTGASQSDGQWF
                     EVDMIGTNTFYELSLDAAGSSNDYPRGYQVNVSNDGSNWGSPVATGSGTSALTLITFP
                     SQTARYIRVTQTGNNSLWWSVHEFNVVGVNGAPPLAPTGLTAIPGDARVSLSWNSSTG
                     AAGYNVKRSASSNGVYTLIAANLTTLNFTNTGLSNGMMYYFVVSATNAAGESAGTAPI
                     SAQPVSLSPPQLAFSAGAGRLQLAWPQDHLGWTLQVQTNSPGAGLGTNWTTVQSSAST
                     NQIAFPINSNNGSVFFRLVHP"
     gene            4971..6656
                     /locus_tag="VG754_13695"
     CDS             4971..6656
                     /locus_tag="VG754_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWF20318.1"
                     /translation="MAKKRGIVILSFYAGCILAAGDFSVYSATKSLEIQSVTINGSPV
                     PLHGDGSVSLGPFPENISFGFQPGTNSAPPIRLRYKLEGRDNEWHEGGGEMGLSIRFY
                     TESGDQISQKIFNVRGESAGWAGSMENSPLSHRRETLVVPPQASRVWVVISSAGPPAC
                     VGIYVVANLLVSKTTGSGPPAVLIQSPFDSPPYRADTNGIPTGWTPDGIRPSMAKIVT
                     FGQEPAINAFAILDDDPISHAEWRTVKEFAPAVSPGDHLVVEWNEMYSMGVGDFRSVN
                     YQKLPRGTFTFRLMEVDLMGVPTGVETSLAVSVPPPFWRTSWFWSLVLISVTGATVGG
                     ARYLGWQKMRREMLRLKAQQSLERERLRIAHDIHDDLGARVTQISLVSAMSQENPTFP
                     EKARADFDRISRMSRELVAALYETVWAVSPENDNLDALGNYLCQMVNQLCEQSRFRCR
                     FDMLDLPREIQVSSQIRHNIIMAVKEAVHNVIKHARASEVTLHIGFSENEIVLLVQDD
                     GCGFDPAKSSAGHGLSNMKRRLQDVGGSCVIKSQLGKGTIVHIRLSFNSSKVP"
     gene            6653..7285
                     /locus_tag="VG754_13700"
     CDS             6653..7285
                     /locus_tag="VG754_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF20319.1"
                     /translation="MKKTVVIVEDDGGLREQLVQILDTASDIKCLGAFRSGEEALPQI
                     LEKKPDVVLMDIKLPGMSGIQCVAEVKKIFPEMQIIMVTIYEDSERIFRALKAGANGY
                     LVKSGPPEQLLESIRDVHKGGAPMSSPIARKVVKHFHLIGPSVTETENLSPREREVLD
                     LLGQGFIYKEIGDKLNIGTETVRTYVKNICQKMHVRGRIEAVAKHRAELS"
BASE COUNT         1864 a         2170 c         2084 g         1766 t
ORIGIN      
        1 gctcgatgct gctcaagggc ggttcacaca aattgcaaag cacgtcatca ttgtccactc
       61 ccaggaccgc gacttcatcc ggcactttga tgtcatgttc gcggcaggca ttgagaacct
      121 gctgaccgca tacatcatta caggcgaaca gcgccagcgg gcgaggttgt ttgcgcaacc
      181 aggctgcgat tgcccgctct cttcccaatc cgcgccgctc gaccgcctgg atgtccgatg
      241 gcaatcggga caaaccgtcg tccgtttcaa cggaaccgga aaccggcgga aacacgcgca
      301 gcttgtaacc ttgagtacgc aaacaactgg cgaacgcagc ggcccgcctg tccgagaacg
      361 ggatgttcca atagccgcag aaagcaaaat gccggaatcc cgcccggata aaaaaatccg
      421 ccgcgagccg ggccacttca cgcgcatcgg tgtcaaaccc ggggattccc ttgaactggc
      481 agtttccgag aacatcaacg accgggaagc ccaggcgcaa aagcgaattg gcggcgcgct
      541 tgtcctcgat gcgcgcgatg atgccgtgac ctttccattc tttgagccaa tcgggaattc
      601 cgctgtgcag ttcgcgttcc tcggtgaagc acgaccatgg cccgtgcaga tgggtgtatg
      661 ccgcgatgcc gcgcaagatg ccgcgtccgt aaatcctcga actttcgatc aacaatgcga
      721 tcctgggcaa attgcacgac ataagctctg ttcgtttcgg aagttcgaat acattgatga
      781 tgagaaattg cttgtcgcat atattaaacg ttttgacaag aatcgaaaat gcggtttatc
      841 aaacatcccc tatttcgggg gtatttgttc cgcttgtgtt tgactacttt cattggcttg
      901 attccaattt gatccggaaa tgaaaaaaat tatcttcagt tgccgcgccc gcaagaagat
      961 tacctcggaa agcagcgcga tcggcgaatc ctttaaatcg aggtgaggcg aatggaaaaa
     1021 tctcctctat cgaatacgaa aagagcatcc tgtttgctgg ctgctgcgct ttcacttttt
     1081 aatcatcctc acatggcgca cgcgggcgtt ggcgccacga ctccgttcat cagctttgag
     1141 gcggaaagcg ggtttctggg cggcggcgcc acaatggttt ctgtcacttc tgccccgacc
     1201 aatcaatatt cgagtcccga gctggaggca tccggccacg cctatgttca actcacggcc
     1261 acaaatcaat acgtgcaatg gaccaataac acgggtcaga gccttacggc cataaacctt
     1321 cgctcctgta ttccggatgc tccggctgga ggagggatca cctccacgat tgatctctac
     1381 gtgaatggcg tattccgtca ggcgtttgcg gtcaactccc aacaaaatta ttgttacgaa
     1441 ggcaccaatt acaacggtca gaccgacaag aatcctgccg acggcgatcc ccgcggtttc
     1501 tggaatgaca cgcatgcttt cattaccggc gcgcctattg ctccaggcga cacattccgt
     1561 ttccagatgg ataccgcgaa caccgcggct ttttacgaca tagacgtcgt ggatcttgaa
     1621 actccgcctt cgccgctggt ccaaccttcc aactcccttt ccattctcag ttacgaagcg
     1681 gtatcgaaca acattggcgt ggacaatacg tccgccatca acaactgctt caccgccgca
     1741 agatctcagg ggaagagtgc ctggattccc gcaggaacgt tttacatcag tgccatacag
     1801 ggcggcctta acgctgctgg gataaccata gtgggcgcgg gcccttggta tagcacgatt
     1861 aaccgcgtaa ccccggccaa taattcacaa ggcgtgaaca atatcatcac caccgtttca
     1921 tgcacacttt cgaacgtgtc gctggattgc aacggctcaa gccgggcagg aaacaacaac
     1981 aacggcgcag tcaatttctc cgggaccaac tgggtcgtgg acaacgtctg gattcaacat
     2041 gtgacttcgg ccttctggtg cgccggtgtg ggcgggacgg cgcgcaattg ccgtgtgtta
     2101 agcgtctggt ccgacggcgg caacttcaac aatgtgcaga gcgacgacgg catcggaatg
     2161 aacctgactt actcgaacaa ctttgtcagg ggcaccggcg atgacgcgat ggcaatcaat
     2221 tcggtgaact acaatgtgtt cggctcgacg acctattatt acacgatgat gagcaacatc
     2281 acgtacgtga acaacacggc cattgcccct tggggtggca agtgcattgg cgtttacggc
     2341 ggcgtcaacg acttggtgac gaacaatctg ctttgcgaca cggcaagata tatcggtctt
     2401 gggataatga agtttggcgt gaacggctca gatcttttgt cggccacagt gacaggaaat
     2461 accgtgctcc gctgcggagg caacggctac aatcaacagc agcaggcgtt gatgatcggc
     2521 aacggcggcg acggccaagg cgtgggaacg gtcgcaaatg tttattgcgc gtcgaacacc
     2581 atcattaacg ccatgtatga cgcaataggt ttttcgacca gcaccaacat tcttttccag
     2641 cggaatacca tcataaatcc cgcgctggat gccattgcca tcgggccacc ggacctcggt
     2701 tcgagtgtca tggggaacgc gctgatttat tcgaacagcg tctcgggcct caaaaccgga
     2761 caggtggtgt ttaccaacag ctcagtcggt tatgccggca tcatcccgac agccgccaca
     2821 agctacaaca atatgtcggg cgtcgctccg gagaattgca gcgaaggagg acaggacatc
     2881 ggcaatatca cggcgggaga ctgggttgcc tacaacaacg tcaatcttgg cggcataaat
     2941 gccttcgtcg tcaggatcgc cagcaccaat tccggggccg gcattcaagt ccgccttgac
     3001 ggcgccaacg gaaccctgat tgggaactgt gtcgttccga ttacaggcgg atcgcaaacc
     3061 tacgcaaacg catattgcag tgtcagcaac gcgagtggga cgcataccgt ttatctcgta
     3121 ttttctggcg ggaacggaaa tttatgcagt gtggaatttt tcgctttgta ttacgcgcct
     3181 ccggacctct cccatcaatt ggtggcaggt gatgtctatt cgttgaacgc ggtcggcaat
     3241 ggaaagtacg tcactgagga cagcagttat accaatgtat tgtatgctga aggaaccaca
     3301 gtcagcgctc cgcaagagtt ccagattgtt gatgcaggcg gcggcaacat tgcgctccaa
     3361 tccttggcca atgacgacta cgtcagcgcg gataacaatg gcaattcccc gttggtcgcc
     3421 aacagaaccg gtgtgggctt gtgggaaaca ttcacggaag tggatgccgg aaatgggaat
     3481 atcgccttgc gggcgatgaa caacggcaag tacgtcaccg tggacagctt gaatggcaat
     3541 gttttgatcg ccaaggcaac aacggttggc cctcaagaat ctttcacagt cgcattcgtc
     3601 agcggcgtgg caccctctgc gcccgcaggc ctgactgcat cgcccggcaa ttctcaagtt
     3661 gttttaagtt ggaccgcatc cccgggcgca accgcataca atatcaggcg atccaccacc
     3721 aacaacgggc cttacggaat cgtggcatcc aatataacga cgttgaccta tacaaatttc
     3781 aacctgacca acggaattac atattattac gtggtgtccg cgtttaatcc ggcaggcgaa
     3841 agcacaaatt ctacgcaggt caccgccatc ccgggcgcac tggaccgttc cggttggttg
     3901 gcgagcgctt cttccacaga atccggcggc tcgccttcgt atgcgattga cggcaatgtc
     3961 agcacgcgat ggtccaccgg cgcgtcacaa agcgacgggc aatggttcga ggtggacatg
     4021 atcgggacga atacctttta cgagttgtcg ttggatgccg cgggttcgtc gaatgattat
     4081 ccgcgcggct atcaggtcaa tgtctccaac gacggcagca actggggttc cccggtggcg
     4141 accggttctg gaacttccgc cctgacactg atcacatttc cgtcccaaac ggcccgctac
     4201 atccgggtga cgcaaaccgg aaacaattca ctatggtgga gcgttcacga attcaatgtg
     4261 gttggcgtaa acggcgcgcc gccacttgct ccaacgggat tgactgcaat ccccggagat
     4321 gcccgcgtat cgttatcatg gaactcatcc actggcgccg caggctacaa tgtcaagcgt
     4381 tccgcttctt cgaatggagt ttacaccctc atcgcagcga acctcacgac actcaatttc
     4441 accaacacgg gcttgagcaa cgggatgatg tactatttcg ttgtatccgc aacaaatgcg
     4501 gccggtgaga gcgcaggcac cgctcccatc agcgctcaac cggtttccct ttctccgccg
     4561 caactcgctt tttcggcagg agcgggccga ttacaactcg catggcctca agatcatctc
     4621 ggttggactt tgcaagtgca gaccaactcg cccggcgcgg gcttgggcac aaattggaca
     4681 accgtgcaat cttcggcgtc aacgaaccaa atcgcgtttc caataaactc aaacaacggc
     4741 agcgtctttt tccgattggt gcatccataa gagcagtggt ttgtttgtcc gatgccatcc
     4801 atccccgaaa acggggatgc tcagcaagac aatatgttga ttagctcaag tcaaatcctt
     4861 ttggcatcat gtcaatgtgt ttgagttgca atttcgaacc ggtgaattca aaatcccggc
     4921 atgaccgcca gtctttcacc tgctcaagac aactatcaac ttctgatgag atggccaaga
     4981 aacgtggcat agtcatttta tcgttctacg ccggctgtat tttggcggcc ggcgatttct
     5041 ctgtttattc cgccaccaag tccctcgaaa tccaatcggt gacgatcaac ggcagtcccg
     5101 ttcctctgca cggcgatggg agtgtgagtt tggggccatt tccggaaaat atttcctttg
     5161 gttttcaacc gggcacaaac tctgccccgc caatccgcct tcgctacaaa ctggaaggca
     5221 gggataacga atggcacgaa ggcggcggcg agatgggttt gagcatccgc ttttacactg
     5281 aatcgggaga ccagataagc cagaaaattt tcaatgtccg cggcgaaagc gcgggctggg
     5341 ccggctcgat ggaaaattct ccactctccc atcgccgtga aactctggtt gtgccgccgc
     5401 aggcctcgcg agtatgggtc gtgatttctt ccgcagggcc gcccgcctgt gttggaattt
     5461 atgtcgtcgc caatttgctc gtctcgaaaa cgacaggcag cggcccgcca gcggtgttga
     5521 ttcaatctcc ttttgattcg ccgccttatc gggccgatac gaatggaatc cccaccggat
     5581 ggacgcccga cggcatccgt ccgagcatgg caaagattgt cacgtttggc caggaacccg
     5641 ccataaacgc gtttgcgatc ctcgatgatg atcccatcag ccatgccgaa tggcgcaccg
     5701 tcaaggaatt cgcaccggca gtcagtcccg gcgatcatct cgtcgttgaa tggaacgaaa
     5761 tgtacagcat gggagtcggc gacttccgct ccgtcaatta tcaaaagctg ccgcgtggaa
     5821 catttacctt tcgcctcatg gaagtggact tgatgggagt gcccaccggc gtggaaacct
     5881 cgttggccgt gtcggtgccg cctccattct ggcgcacgtc gtggttttgg agccttgttt
     5941 tgatttccgt cacgggcgcc acggtcggag gagcgcggta tctgggatgg caaaaaatgc
     6001 ggcgcgaaat gttgcgcctc aaagcgcagc aatcgttgga acgcgagcgg ttgcgcatcg
     6061 cgcatgacat ccatgacgac ctcggcgctc gcgtgacgca gatttcgctg gtcagcgcga
     6121 tgtcgcagga aaatcccact tttccggaga aggcgcgcgc tgattttgat cgcatttccc
     6181 ggatgtcccg cgaattggtc gccgcgcttt atgaaaccgt ttgggcggtc agcccggaga
     6241 acgacaatct cgatgcgctc ggaaattacc tttgccagat ggtcaaccag ctttgcgaac
     6301 aaagccggtt tcgctgccgt tttgacatgc tggatttgcc gcgcgaaatc caggtgtcca
     6361 gccagatccg ccacaatatc atcatggcgg ttaaggaagc cgttcataat gtcatcaaac
     6421 atgcccgcgc gtccgaggtg acgctccaca tcggattcag cgaaaatgaa atcgttcttt
     6481 tggtgcagga cgacggctgc gggtttgatc ccgccaaaag ttcggccggc cacggattga
     6541 gcaacatgaa gcggcggctg caagacgttg gcggaagttg tgtcatcaaa agccagcttg
     6601 gcaaaggcac aatcgttcat attcggttgt cattcaattc atccaaagtt ccatgaaaaa
     6661 aaccgtggtc atcgttgagg acgacggagg attgcgcgag caactcgtgc aaatcctcga
     6721 caccgcgtcc gacatcaaat gccttggcgc ctttcgctcc ggtgaggaag cgttgccgca
     6781 aatcctggaa aaaaaaccgg acgtcgtcct gatggacatc aagctgcccg gcatgtccgg
     6841 catccaatgc gtcgcggagg tcaaaaagat ttttcccgag atgcagatca tcatggtcac
     6901 aatttatgag gacagtgagc gaattttccg cgcgctcaag gcgggcgcga atgggtatct
     6961 tgtgaagtcc gggccaccgg aacaattgct tgaatccatt cgcgacgttc ataaaggcgg
     7021 cgcgcccatg tccagcccca tcgcgcgcaa ggtcgtcaaa cattttcatt tgattggtcc
     7081 ttccgtgaca gaaacagaaa atctttctcc gcgcgaaagg gaagtcttgg atttgctggg
     7141 ccagggattc atctacaagg agatcggcga caaactgaac atcggcaccg aaaccgtgcg
     7201 cacctacgtc aagaacatct gccagaagat gcatgtgcgc ggccgcatcg aggcggtcgc
     7261 caagcaccgg gcggagttga gttgagcaga taaaaacatc gaacaaccaa caccgaactc
     7321 cgaacatcga atgttttgga gtcgccgggg acttccccaa ttcagcgttc gacgttcgat
     7381 gttgggcgtt cgatgtttcc ggaagaattc gcaaaccgtg ccgaggcgag aaaagaattt
     7441 tgttcatttt agccttaatt acccttaatt acccttaatt agccttaatt aggccttaat
     7501 tttccgatgc tggatgctgg attcttggtg ctggatgatg ggcattccgt aatccgcacc
     7561 cccggggggg tgtttttggc ggaattttgt gaaactttat gaaattttgc ggaatttcaa
     7621 ccgcttacaa cttgagccat ttcactcaat ttcaaccaaa ccggatgaaa atattaattt
     7681 ttttatcgcg gtttatcgcg gtcaagcccg gttgatctga aaataaccga aacaacctga
     7741 aacaacgtag ctgaaaacgt gagttttcag gtccttccgg acgggccatg ttcggcgaag
     7801 tccgcccaac gtccaagcat gagagaaccg agacgcgact tgcccgcgaa aaacacgaaa
     7861 gtccgcgaaa tccgcaactt taaa
//
LOCUS       DASZMM010000571         9475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3296432, whole genome shotgun sequence.
ACCESSION   DASZMM010000571 DASZMM010000000
VERSION     DASZMM010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3296432"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VG754_13705"
     CDS             complement(<1..623)
                     /locus_tag="VG754_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HWF20320.1"
                     /translation="MKNLKRPKLKARQAQLIASGDLRLSANQVCWPAQAEMERSLKAA
                     FASEGWQVLRAHPYKAWEKHGFIASQREGMETFQNIDPAAPLIVAEAVWQYSHHVLAG
                     LTTHRGPILTVANWSGQWPGLVGMLNLNGSLTRAGVKYSTLWSEDFTDEYFLAKLRQW
                     LATGKCQHATPHVMPFAKAKLNGSAARLGQTLAADLREDKAIMGVFD"
     gene            complement(1081..1566)
                     /locus_tag="VG754_13710"
     CDS             complement(1081..1566)
                     /locus_tag="VG754_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWF20321.1"
                     /translation="MNFRNKLVSWEQLPAWRAAFRERKQKLVVTNGCFDILHLGHVTY
                     LEAARNLGDALLIGLNADAAVGALKGPNRPVNSEADRAAVLASLASVDGVCIFTERSA
                     TRFLSIAQPDIYVKGGDYTLETINQEERRQVEKSGGKVLILPVVPGKSTTALLKKIAQ
                     L"
     gene            complement(1612..2358)
                     /locus_tag="VG754_13715"
     CDS             complement(1612..2358)
                     /locus_tag="VG754_13715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HWF20322.1"
                     /translation="MISSESVTNKFYETGERRAERVKDLFATIAPRYDFINDLQSGGL
                     HRIWKRRLLTLAAGRPGERVLDLCCGTGDVALAFAQQGLRVVGLDFSEPMLAVARERV
                     KSPKFTDGGAVEFLQGDALRAPFPDASFDIVTISYGLRNLADWEAGLREMRRLARPGG
                     RLLVLDFGKPDNALWRGIYFSYLRWCVPVLGKIFCGDSDTHGYILESLTHYAAQRGVE
                     AKMKELSLAHTRLVNLLGGVMSINYAEKPR"
     gene            2627..3151
                     /locus_tag="VG754_13720"
     CDS             2627..3151
                     /locus_tag="VG754_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF20323.1"
                     /translation="MKMFGKWRNEPESVPQEEAAFAGDIAPAKAGQPLIGEGRKILVV
                     DDNKVVLKAFELKMKNLGFTVLTALDGPAAVSCARHEKPDVIVLDINFPPDVGSTGLQ
                     WDGFTILLWMRRFQDAANIPVIIITSGDPSKFKDKAMTAGAVAFFQKPIDNEDFIMAL
                     RRVVGQPVKNSKAA"
     gene            complement(3160..4224)
                     /locus_tag="VG754_13725"
     CDS             complement(3160..4224)
                     /locus_tag="VG754_13725"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWF20324.1"
                     /translation="MGNSPMQALRLRVWRLGNCGLSLCIMGKFSREKHMPWIRIGCAA
                     LALAALVYVFSQINLHELAKALRSARVGWLIATIAVYWFVLLPSAWRWHLALELTSCA
                     VRFGTTLRMTLIGHLFYTLFFGVAGGDVAKSALYARRNRLPLPEVLAAAPLDRLLGFG
                     GLVIFIIASFALAAANGAFSQFRPASLKLPASWTALAVVLAAIFFIAWFVRRRSGHET
                     AFGRLVLTFLKGWRRLVKSPRILWQGLLCGLAVQLALAASLAFGLEAVSNSPVPWGRL
                     IWTLPVISVASALPVNIAGMGLREGAVLALLGLYGVSPADAVAASLLTLLARLFWAGV
                     GAAALWQRQPTEIVGKPVPG"
     gene            4296..4814
                     /locus_tag="VG754_13730"
     CDS             4296..4814
                     /locus_tag="VG754_13730"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWF20325.1"
                     /translation="MQYKTKRFILAGCLAASVATAGLTGCHTEDRSAGRYMDDRMVAR
                     RVEGNLKRDPVYKYPDVRVSVFEGVAQLSGFVDSQRQIATAAEHASRTDGVREVINDI
                     SLKPQFKLVPAANEQSGRRWESNTGGTGSNVGENTGKYNETAPQQQRAPIITPNQATP
                     ATPPTSNSGNNQ"
     gene            4941..5804
                     /locus_tag="VG754_13735"
     CDS             4941..5804
                     /locus_tag="VG754_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWF20326.1"
                     /translation="MTNHRLFQWFKFLPALASLIFLTLPATAAPKPLHVLLITGGCCH
                     DYAAQKDILKKGIEARANVIVDQVHTDDSTTHPPLAILGNPDYAKGYDLVIHDECAAD
                     ISDPALIEMVLKPHRDGIPAVNLHCAMHCYRVGNPNDPVTLGTPHGLWFEYLGLQSSG
                     HGKQIPISLTFTDTASPITKGMSNWTTIHEELYNNIHIFDKTHPLARGKQVTKQKDGS
                     EKVDDFVVVWTNEYGPKKTRVFSTTIGHNNETVSDPRYLDLVTRGVLWATGHLKKDGT
                     PAKGYGPQVAK"
     gene            5881..6309
                     /locus_tag="VG754_13740"
     CDS             5881..6309
                     /locus_tag="VG754_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20327.1"
                     /translation="MLLRYLINLSAGRLILWCYFIWYVVVLVRYFDISARLWLTSFGL
                     SLIIGTALFISTAKTGANNVKLESWQVFRLFLMPFCVSSFSALVKDKGFVLIFSPKPV
                     EIAIAAALCALLCGTVFILKRRARMTVNAPKKSDEANVRA"
     gene            complement(6341..7189)
                     /locus_tag="VG754_13745"
     CDS             complement(6341..7189)
                     /locus_tag="VG754_13745"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF20328.1"
                     /translation="MRTTVDSSHPAVLVRDLWMSFPGKRAGEEIHVLERVNLEVRAGE
                     FVCIVGPSGCGKSTLLNIIGGFLQATRGQLLVEGQAVAGPDPRRIFVFQENGVFPWLN
                     VQENIGFGLLKKPAHERERIVEHYIDMVGLHGFERAYPRELSGGMRQRVEIARALAAN
                     PDIIYMDEPFGALDFITRLKMRADLVRIWQREKKTILFVTHDIEEAVQLADRVLIMSQ
                     RPATIQTIVLIDLQRPRDLDSKGYLATRDRIFSAMGMSLRIGGNETENAANRIAKNGE
                     LQPPAQ"
     gene            complement(7173..7967)
                     /locus_tag="VG754_13750"
     CDS             complement(7173..7967)
                     /locus_tag="VG754_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF20329.1"
                     /translation="MTEPMAKREPSRNWTRFVLPLGVAAVFLLVWDLAVRASGSDLFP
                     KPIEVLRGILQLIERGLLLKYVVASLFRVTWGFTLAVVVGVPFGLFLGWYGWAFQAFN
                     PMIQMLRPISPIAWIPVAILWFKVTDKAPIFLIFLASVFPITVSAIAAVQHLQPVYLR
                     AARNFGLTQMQLFRRVILPATLPQIITGIRIALGVAWLVVVAAEMIAVNSGLGYLIID
                     ARNAGKRYDLVVAGMVMIGLIGLVLDLLVRQLEKFDEVRWGYANHR"
     gene            complement(8048..9127)
                     /locus_tag="VG754_13755"
     CDS             complement(8048..9127)
                     /locus_tag="VG754_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWF20330.1"
                     /translation="MSFKRTILLAAALWLVLITCLHLTLNLDVHPFARRNAVAERKKF
                     RVGFLPVTCHLTCPVTDFINKNMTGDSPFEPVRFNGWPELKEAFLSGYMPATFILAPL
                     AMALREQGVPIKIVYLGHRDGTAMMVHTNSQIFRVEDLKGKTIAVPNRYSNQRLLIFK
                     ALREHGMSIADVHLVEMPPPDMPVALRSRAVDAITSGEPFMGQTELDGYGRVLYLTKD
                     IWPDFISCVLAVHEDAIKNHRAEVQRLVDGIARSGKWLDQTMDHRMQASQFVAKNYYN
                     QDPRLLAFVLSKPPDRVKYTRLTPLKKDFEEIESLAVEAGIMQGTVKFEDYVDDSFVH
                     NESAIQPYQWEAVTNVASAAATNPK"
BASE COUNT         2239 a         2698 c         2586 g         1952 t
ORIGIN      
        1 tcatcgaaca cgcccatgat ggccttgtcc tcgcgcaaat ccgccgccaa ggtttggccg
       61 agacgcgccg ccgaaccgtt caattttgcc ttcgcgaacg gcatcacatg cggcgtcgcg
      121 tgctggcatt ttccagtcgc aagccattgg cgaagtttcg ccaaaaaata ttcatcggtg
      181 aaatcctcgc tccacaacgt cgaatacttc actccggcgc gcgtgagcga gccgttgagg
      241 ttcaacatcc cgaccaatcc gggccattgg ccggaccaat tcgccaccgt gagaatcgga
      301 ccgcgatgtg tcgtcaggcc agcgagaacg tgatgcgaat attgccagac cgcctcggcc
      361 acaatcagcg gggcagcggg atcgatgttc tggaacgtct ccatcccttc gcgttgcgag
      421 gcgatgaacc cgtgcttctc ccaagcttta tacggatgcg ctcgcaacac ctgccagcct
      481 tccgatgcga acgccgcctt caggcttcgc tccatctccg cctgcgcggg ccagcaaacc
      541 tggttggccg agagacgcaa atcgccgctg gcaatgagct gggcctgacg cgctttcaac
      601 ttgggccgtt taagattttt catcataggc gggcgcatcg taagccgaat ccatggcagt
      661 ggcaatccgt gtgaggtatc tccggcaagc gacacgattc ggcgaaaaat gcggttgtag
      721 cagtttgggg agtgcgtatg cccgtgcgga agcacctcgg gcgttctccc cctgactgca
      781 cagaatagag gcacgtcttg actcgcgtga gcgatggaac gatgggtaat tttagaagca
      841 aaaaacactg aagcgaatgt aagcaacttt aataaattag gaccggagga gacacttgaa
      901 atcagagtgg cacgcgatca gatcgaagaa attttgcggc agcatcctga atatgaatgg
      961 tcatatttga taagccacaa cacctttgca cgttgattga caaataatat gcggccaatg
     1021 gaacgcggac agatgggatt gagattcttt cttatctgcc ttggtgtctt ggtggttaaa
     1081 tcaaagctgt gcgatttttt tcaacaaggc ggtcgtggat tttcccggca cgacaggcag
     1141 gatgagcact ttgccgccgg atttttccac ttgccggcgt tcttcctgat tgatggtttc
     1201 gagcgtgtag tcgccgcctt tcacatagat gtccggttgc gcaatcgaaa gaaaacgcgt
     1261 tgcggaacgt tcggtaaaaa tgcatacgcc atccacgctc gccagcgaag cgagcacggc
     1321 ggcgcggtcg gcttcggaat tcaccgggcg gttcggacct ttcaacgcgc ccacggcggc
     1381 atcagcgttc aacccgatca gcaaggcatc gccgagattg cgggcagctt ccagataagt
     1441 cacatggcca agatggagga tgtcaaagca gccgttggtg acgacgagct tttgcttgcg
     1501 ttcgcgaaaa gccgcgcgcc acgcgggcaa ctgctcccag gaaaccagct tgttccgaaa
     1561 attcaccaca ctgaatattg gccgatatgt ttcgcggttg gcaacgaact tttagcgcgg
     1621 cttctccgcg taattgatgc tcatcacgcc gcccagcaaa ttcaccaatc gcgtgtgcgc
     1681 caggctcaat tccttcatct tcgcctccac gcctcgttgc gcggcgtaat gcgtcaacga
     1741 ttccaagatg tagccatgcg tgtcagaatc gccgcagaaa atttttccca gcaccggcac
     1801 gcaccagcgc aggtaggaga aataaattcc gcgccacaag gcgttgtccg gcttgccgaa
     1861 atccagcacc agcagccgtc cacccggacg cgccaaccgg cgcatctctc ggagaccggc
     1921 ttcccaatcc gccaggttgc gcaggccata actgatcgtc acgatatcaa agcttgcgtc
     1981 cggaaatggc gcgcgcagcg catccccctg caagaattcc acagcgcctc catctgtaaa
     2041 cttgggactt ttaacccttt cccgcgccac tgccagcatc ggctcgctga aatccaaccc
     2101 gacgactcgc aagccttgtt gcgcgaaggc aagcgcgaca tcgcctgtgc cgcaacaaag
     2161 atccaacacg cgttcaccgg gacggcccgc cgcgagcgtc agcagacgtc gcttccaaat
     2221 acgatgcaag ccgccgcttt gcaggtcatt gatgaagtcg tagcgcggcg caatggtggc
     2281 gaacaggtct tttacccgtt cggcccggcg ttctccggtc tcgtaaaatt tgttcgtcac
     2341 gctttcgctg ctaatcatcg gcttcttgcc ggacggatgc caatcctttc aattaaaact
     2401 caatcgctat tacccaaaaa tgccatattc gctggcacga taaaggctat tacaagatgt
     2461 cggatttgag aatgtatgat gaaaacaatg aaatctgcct ttaatagcag cgatttcagc
     2521 acacactgga ctgtttgcga gcttggggtt agttcgaaga gcttgcgagc cgggtgtcca
     2581 gtaaatgtac acatacgcga ttaaactgcc ggaaatgtaa ccacctatga aaatgttcgg
     2641 aaaatggcgc aacgaacccg aatcagtacc acaagaagaa gctgctttcg ccggggacat
     2701 agctcccgca aaggcaggcc aaccgctgat tggtgagggc cggaaaatcc tggtcgtgga
     2761 tgataacaaa gtggttttga aggcatttga actcaagatg aaaaaccttg ggttcaccgt
     2821 ccttaccgcg ctcgacggcc cggcggctgt cagttgcgcc cggcatgaaa agccggatgt
     2881 gatcgtgctg gacatcaatt tcccgccgga tgtgggcagc acgggtttgc aatgggacgg
     2941 tttcaccatt ttgctatgga tgcgacgttt tcaagatgcc gccaatatcc cggtcatcat
     3001 catcacgtcc ggcgatccgt ccaagttcaa ggacaaggcg atgacggctg gcgcggtcgc
     3061 attttttcag aagccgattg acaacgaaga tttcatcatg gcgctgcggc gcgtggtcgg
     3121 ccaaccggta aaaaattcca aggcggcctg atcccgcatt caacccggca caggtttgcc
     3181 cacgatttct gtcggctgcc tctgccataa cgccgcggct ccgacacccg cccagaacaa
     3241 tctcgccagg agtgtcagca gcgacgccgc cacagcgtcc gcgggagaaa ctccgtaaag
     3301 accgagcaac gccaagactg cgccttcgcg caagcccatg cccgcgatgt tcaccggcaa
     3361 tgcgcttgcg acagaaatca ccggcaacgt ccagatcaat cggccccaag ggaccggcga
     3421 gttgctgacc gcctcgagcc caaaagccag cgacgcagcc aaagccagtt gcaccgccaa
     3481 tccgcagagc aatccctgcc ataaaattcg gggtgacttc accagccttc gccaaccttt
     3541 caaaaacgtc agcaccaatc tgccaaaggc ggtctcgtgt ccggaacgcc ttcgcacaaa
     3601 ccatgcgatg aaaaaaatcg cggcgagcac aacggccagg gctgtccaac tcgcaggcaa
     3661 tttcaacgaa gctggtctga attgcgagaa cgcgccgttt gccgctgcca gcgcaaacga
     3721 tgcgattatg aatatcacca atccaccgaa gcccagcagc cgatccaatg gcgctgccgc
     3781 caggacttcg ggaagtggca accgatttcg gcgcgcgtat agcgccgatt tcgcgacgtc
     3841 gcctcccgcc acgccaaaaa aaagcgtgta gaacaaatgg ccgatcaaag tcatccgcag
     3901 tgttgttccg aaccgcaccg cgcaacttgt caattccagc gcgagatgcc atcgccatgc
     3961 cgatggcagc aacacgaacc aatacaccgc gatcgttgcg atgagccagc cgacacgcgc
     4021 cgagcgcagg gcctttgcaa gctcgtgaag attgatttgg ctgaacacat aaacaagcgc
     4081 tgccagcgcg agcgccgcgc aaccgatgcg gatccatggc atgtgttttt cccgtgagaa
     4141 ttttcccatg atgcacaagc ttaaaccgca atttcccaaa cgccaaacgc gcaaccgcag
     4201 ggcctgcata ggcgaattcc ccatggctgc ggcgaagcca agaagtaaat ttctgcaagg
     4261 aaacgtaaac gccttaaaaa aacgaaagct gacctatgca atacaaaaca aaacgattca
     4321 ttctcgcggg gtgccttgct gcctccgtcg caaccgccgg cctgaccgga tgtcacactg
     4381 aagaccgcag cgccgggcgc tacatggacg accgcatggt cgcccgccgc gttgagggca
     4441 atctcaagcg ggatccggtt tacaaatatc ctgacgtgag ggtgagcgtc tttgaaggcg
     4501 ttgcccaact cagcggcttt gttgattctc aacggcagat cgccacggcc gcagagcatg
     4561 cgtccaggac agatggagtc cgcgaagtga tcaacgacat ttcgctcaaa ccgcaattca
     4621 agcttgttcc cgccgccaat gagcagtcgg gacgccgttg ggaatccaat accggtggca
     4681 ctggctcgaa tgtgggtgaa aataccggca agtataatga aacggccccg cagcaacagc
     4741 gcgctccgat catcactccc aatcaggcca cgccggccac tcccccgacg agcaacagtg
     4801 gcaataacca ataaacagcc gaacatttga attaaaacgg gcgcggctcc cgcttcactc
     4861 cgggagacgc gctttttcgc gcgccgacgc tcattcaacc cgcgcttgca accatttccg
     4921 caaaccgcta caaacggcgc atgaccaatc accgtctgtt tcaatggttc aaatttctcc
     4981 cggcgcttgc atctttgatc ttcttgacct tgcctgccac ggcagcgccc aaacctttgc
     5041 atgtcctgct catcaccggc ggttgttgcc atgattacgc cgcgcaaaag gacatcctta
     5101 aaaaaggcat cgaggcgcgc gccaatgtta tcgttgatca agtccacacg gacgattcga
     5161 ccacgcatcc gccgctcgcc atccttggca atcctgatta cgcaaaaggc tacgacctcg
     5221 tcatccacga cgaatgcgct gcggacattt ccgacccggc cttgatcgaa atggtgctca
     5281 agccgcatcg cgacggcata cccgctgtga acctccattg cgcgatgcat tgctatcgcg
     5341 tcggcaatcc caacgacccc gtcacacttg gcacgccgca cggactttgg ttcgaatatc
     5401 tcggcctgca atccagcggc cacggcaagc aaatccccat ttccctcacc ttcaccgaca
     5461 ctgcaagtcc catcaccaag ggaatgagca attggaccac aatccacgag gagctttaca
     5521 acaacatcca catcttcgac aaaacacatc cgctcgcccg cggcaagcag gtcacgaaac
     5581 aaaaagatgg ttccgagaaa gtggatgatt ttgtggtcgt ctggacgaac gaatacggtc
     5641 ccaagaaaac gcgcgtcttt agcaccacca tcggccacaa caacgaaacg gtttccgatc
     5701 cgcgttacct cgaccttgtc acacgcgggg tgctttgggc cacgggccat cttaagaagg
     5761 acggcactcc tgccaagggc tatggtccgc aggttgccaa atagcgttct tctccgagct
     5821 cgacagccgc tcacaacatt gctgcatttg caggctgcca ttggtctttg cgggctgatt
     5881 atgttgcttc gatacctcat caacttgtcc gcgggacggc tgattttgtg gtgttatttc
     5941 atctggtacg tcgtcgtgct cgtgcgctat tttgatatca gcgcgcggct gtggctgacg
     6001 tcgtttggac tgagcctcat catcgggacc gcgctgttca tcagcacggc gaagaccggc
     6061 gcgaacaacg tgaaacttga aagctggcag gtgttccggt tgttcctgat gccgttttgc
     6121 gtttcgagtt tttcagcgct ggtcaaggac aagggattcg tgctcatttt ttcgccgaaa
     6181 ccggtggaga tcgcgattgc ggcggccttg tgcgcgttat tgtgcgggac ggtttttatt
     6241 ttaaaacgac gcgcgcggat gacggtgaac gctcccaaaa aatcggacga agcaaatgtg
     6301 cgcgcataga atcaccaatt tcaaatatga aatttgaaat ttattgggcg ggtggctgca
     6361 actctccatt ctttgcaatt cgattggctg cattttccgt ttcgttgccg ccgatgcgca
     6421 ggctcattcc catggctgaa aaaatccggt cgcgcgtcgc gagataaccc ttggaatcca
     6481 ggtcgcgcgg acgctgcaaa tcaatcagca caatggtctg gatggtggcg ggacgctggc
     6541 tcatgatgag cacgcggtcg gcaagctgca cggcttcctc gatgtcgtgc gtgacgaaca
     6601 gaatggtttt cttctcccgt tgccagatgc ggaccaaatc agcgcgcatt ttcaggcgcg
     6661 tgatgaaatc aagagcgccg aacggttcgt ccatgtagat gatgtcggga ttggccgcaa
     6721 gcgcgcgggc gatctcaacc cgctgccgca tcccgccgga aagttcgcgc ggataagcgc
     6781 gttcgaaacc gtgcaggccg accatgtcaa tgtaatgctc cacgatgcgt tcgcgttcgt
     6841 gcgccggttt tttcagcagg ccgaaaccga tgttttcctg cacgttgagc cacggaaaaa
     6901 cgccgttttc ctggaacaca aaaatacgcc gcggatcagg acccgcgacc gcttggcctt
     6961 cgaccagcaa ctgaccgcgc gtggcttgga gaaagccgcc gatgatattg agcagcgtgg
     7021 atttaccgca acccgaaggg ccgacgatgc agacaaattc acccgcgcga acttccagat
     7081 tcacgcgttc gaggacatgg atttcttcac cggcgcgttt gccgggaaaa ctcatccaaa
     7141 gatcgcgcac caggacggcg ggatgggagg aatcaacggt ggttcgcata gccccagcgg
     7201 acttcatcaa atttttccaa ttgccggacg agcaaatcaa ggacgagacc aataagaccg
     7261 atcatcacca tgccggcgac gacgaggtcg tagcgtttgc cggcgttgcg cgcgtcaatg
     7321 atgagatagc caaggccgga attgacggca atcatctcgg cggcgacgac caccagccac
     7381 gcgacaccca atgcgatgcg gatgccggtg atgatttgcg gcagcgtcgc gggcaggatg
     7441 acgcggcgga aaagctgcat ttgcgtcaac ccaaaattgc gggctgcgcg caagtaaacc
     7501 ggctgcagat gttgcaccgc tgcgattgcc gaaaccgtga ttggaaagac gctggcgaga
     7561 aaaatcaaaa aaatgggcgc cttgtctgtc accttgaacc agaggatggc gacgggaatc
     7621 catgcgatgg gcgagatggg ccgcaacatt tgaatcatcg gattgaacgc ttgaaatgcc
     7681 catccgtacc agccgagaaa aagtccgaac ggcacgccca caaccaccgc cagcgtgaaa
     7741 ccccatgtga cgcggaagag agaggcaacg acatatttca aaagcaaccc gcgttcgatg
     7801 agttgcagaa tgccgcgcag cacttcgatg ggtttgggaa aaagatcact gcccgaagcg
     7861 cgcacggcga ggtcccaaac cagaaggaat accgccgcga ctcccagcgg caaaacgaag
     7921 cgcgtccaat tgcgagaggg ttcgcgtttg gccatcggtt ccgtcatgtc aggttcaaaa
     7981 acagcaactg gagtaggcgc gcttgtttca gcgggcgctg ggttgagaat gggaacgtcg
     8041 gttttcatca ttttggattc gtcgccgctg cggatgcaac attggtgaca gcctcccatt
     8101 gataaggctg gatggcggat tcattatgta cgaacgagtc gtccacgtaa tcttcaaatt
     8161 tcaccgtgcc ctgcatgatg cctgcttcaa cggcgagcga ttcaatctcc tcaaaatctt
     8221 ttttcaacgg agtgagccgc gtgtatttga cgcgatcagg cggcttgctc aaaacaaaag
     8281 ccagcaggcg cgggtcctgg ttgtagtaat tcttggcgac gaattgcgag gcttgcatcc
     8341 gatggtccat ggtttggtca agccatttgc cgctgcgggc gatgccgtcc acgagacgct
     8401 gcacttccgc gcgatggttt ttgatcgcgt cttcatgcac cgccaggacg caggagatga
     8461 aatcgggcca gatgtccttg gtgagataaa gaacacggcc gtaaccatcc agttcggttt
     8521 ggcccatgaa cggctcgccg gaagtgatgg cgtccacagc gcgggaacgc aaggcaacgg
     8581 gcatgtcggg cgggggcatt tcgacgaggt gaacatccgc gatgctcatt ccgtgttcgc
     8641 gcagggcctt gaagatgagc aggcgctggt tggaatagcg gttggggacg gcgatggttt
     8701 tgcctttcaa atcttcgacg cgaaaaattt gcgaattggt gtgcaccatc atcgcggtgc
     8761 cgtcgcgatg accgagataa acaatcttga tgggcacgcc ttgttcgcgc agagccatcg
     8821 ccagcggagc gaggatgaag gtcgcgggca tgtaacctga gagaaaagct tccttgagtt
     8881 cgggccagcc attgaagcgc accggttcaa agggactgtc gccggtcatg tttttgttga
     8941 tgaaatcggt caccggacag gtgagatggc aggtgacggg caggaacccg acacggaatt
     9001 tcttacgctc ggcaactgca ttgcgccggg cgaagggatg gacatccagg ttcaaagtaa
     9061 ggtgcaggca cgtgatgagc acgagccaaa gcgctgctgc aagcagaatt gtgcgcttga
     9121 aagacatctt ggggacggaa ggtaaatcaa ggcggcaggt ttgtcacttg gagagtgggc
     9181 gacttcaaac ggaatgttct cataaaagat ttcttttttt atcgcggttt accccggtca
     9241 aggccggttt ttccgccttg gtttgatcgc caagcgacac gaagcgacac ggcgggacca
     9301 agcaaggaaa acatcgaaca accaacgctg aacctcgaac atcgaatgtt gggaccctcg
     9361 gcgttatccg attcagcgtt cgatgttcga tgttgggcgt tcgatgtttg agcccctccc
     9421 cccaacattt tcggcggatt aaagcgggtt taagtgattc taagcgggtt ttcaa
//
LOCUS       DASZMM010000572         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3552515, whole genome shotgun sequence.
ACCESSION   DASZMM010000572 DASZMM010000000
VERSION     DASZMM010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3552515"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VG754_13760"
     CDS             complement(<1..324)
                     /locus_tag="VG754_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20331.1"
                     /translation="MKTLFLTLALLAALAGSVSAQVTVEVLMGQDQFVANESIPIKVR
                     LVNHSGQTLLFGQEDWLSYSVEARDGLVVLKSGEPPQPHDFDVKSAEVATTPRTDLAP
                     YFTIAQ"
     gene            complement(447..899)
                     /locus_tag="VG754_13765"
     CDS             complement(447..899)
                     /locus_tag="VG754_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF20332.1"
                     /translation="MTAPINVILYAEDDENDAFLVQHAFAKVGIPNPLVIVASGRAAI
                     EYLSVGQSPKPCLVLLDLNMPGTSGLEVLKWIRTTPGLSTLVVVMLTSSNQEADIHRA
                     YLQGANGYLVKPKKIEELVSMVRAIKDFWLVQNRANFRAQIPPPKQNG"
     gene            complement(1105..>3464)
                     /locus_tag="VG754_13770"
     CDS             complement(1105..>3464)
                     /locus_tag="VG754_13770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWF20333.1"
                     /translation="LAQQAEARQRELLTTSLKSIGDAVILTDAQGRVTFLNAEAERLT
                     AWTSAEALGRPLPEVFRIANEHTRQTVENPVEKVVRLGSVVGLANHTILLTKDGREIP
                     IDDSAAPIREPGGPMFGIVLVFRDFTERKKAQEADARLAAIVSYSGDAIIAKDLKGIV
                     RSWNAGAERLFGYRPEEIVGKPVTTLFPQDRLKEEDHILDNLRQGRPVERFETVRVAK
                     DGRHIPVLASISPIKDPEGHVIGASKIVHDITDLVAAREALYREKELMTTTLASIGDA
                     VIVTDAQGHVAFLNGEAERLTKWATSDAVDKPLPEIFRIINEKTRHPVENPVEKVLRL
                     GSVVGLANHTVLVAKDGTELPIDDSAAPIQQASGPLYGVVLVFRDFTERKQAEEKLQD
                     LLARFESLVEYAPIAIALFDSELRFISANDRLAEINGLPKDAHFGKRPGELFPGVGER
                     VEEYLRQVRDTGQPVLRVELTGETPAKPGEQRHWIASYYPVRHPNGKVYGIGAMALEV
                     TEQKRAEEALREAHEQLASRALHLERLVQQRTTRLNEMIGDLEGFSYSIVHDMRGPLR
                     AMNSFTQLLQEECGHVSPTAQNYMQRIRTAAVRMDRLIQDGLNYSRLMRAELPLVPVD
                     AAALVRGILETYPMLQPPNATIELSGTFPLVKANEATLTQCVSNLLGNAVKFVAPGVS
                     PHVRVWAEARGERVRLFFTDNGIGIPASAHERIFQIFQRIDNTYEGTGIGLAIVKKAA
                     ERMGGTVGLESEPGKGSTFWIELARAESAESTLNPSSPSGEARIT"
BASE COUNT          728 a         1015 c          975 g          746 t
ORIGIN      
        1 ctgcgcgatg gtgaaatacg gcgcaaggtc cgtgcggggg gtggtggcca cctcggcgga
       61 cttcacatca aaatcatgcg gctgcggcgg ctcgccggac ttgagcacca ccaagccgtc
      121 acgcgcttca actgaatagg agagccagtc ttcctggccg aacaacagtg tctgcccgga
      181 gtgattgacg aggcggacct tgatcggaat ggattcattg gccacgaatt ggtcctggcc
      241 catcaacacc tcgaccgtga cctgcgcgga aacacttccc gccaacgcgg ccaacagcgc
      301 gagcgttaaa aataatgtct tcatcctgtc actttaatga gccgcagcca cgacaagcaa
      361 gcagcaaaac catggttttg cttgcagtcg cgattcaaaa attatgccaa caagcgtcga
      421 atggacgcgg tcacgcagaa atttattcag ccgttctgct tgggtggagg gatctgcgcg
      481 cgaaaattgg cgcggttttg cacgagccaa aaatccttaa tggcgcggac catggaaacc
      541 agttcttcaa tttttttggg ctttacaagg taaccattgg cgccttgaag ataggcgcgg
      601 tggatgtccg cttcctggtt ggaagaagtg agcatcacga ccacaagggt cgagagaccg
      661 ggcgtggtgc ggatccattt caacacttcg agtcctgacg ttcccggcat gttcaagtcc
      721 agcaggacga ggcaaggctt gggagattga ccaaccgaaa gatattcaat cgctgcgcgg
      781 ccgctggcga caatcacaag aggattggga atcccgacct tggcgaaagc gtgttggacg
      841 aggaaagcgt cgttttcatc atcttccgcg tatagaatta catttatcgg agccgtcatg
      901 ccggaaagga gatattacaa gtcatctcat aaacagagaa cggagcgcga ctgtgtccgc
      961 agacccagtc gcagcagctt ggaacactca atatcctggc aggttgctgc ggctggcgct
     1021 ttgcgcacag ccgcgctcca ctatttatga gatgtattct aaactgggtt cgcgattcaa
     1081 tggagttgat catggctcaa aatatcatgt tatccgcgct tcgccagaag ggctggacgg
     1141 attgagcgtg gactcggcac tttcggcgcg ggcaagttcg atccagaaag tgctcccttt
     1201 gcccggctcg gactcaagtc ccacggttcc tcccatgcgt tcggcggcct tcttcacaat
     1261 cgccaacccg atgcccgtgc cttcatacgt attatcgatg cgttgaaaaa tctggaatat
     1321 cctctcatgc gccgaggctg gaatgccaat cccgttgtcc gtgaaaaata atcggacgcg
     1381 ctcgccgcgc gcctccgccc agacgcgcac atgaggcgac acgcccggcg cgacgaactt
     1441 caccgcattg ccaagaagat ttgagacgca ctgggtcagc gtcgcttcgt tggccttgac
     1501 caacggaaag gttccggaca attcgatggt cgcgttgggc ggctggagca tcggataagt
     1561 ttccaaaatg ccacgcacca gggcggccgc gtccacaggc acgagcggca attcagcgcg
     1621 catgaggcgg ctgtaattca acccgtcctg gatcagccgg tccatccgca cggcggcagt
     1681 ccggatgcgt tgcatataat tctgcgccgt cgggctgaca tggccgcatt cttcctggag
     1741 tagttgagtg aagctgttca tcgcccgcaa cggcccgcgc atgtcatgga caatgctgta
     1801 ggaaaaaccc tcgagatcgc cgatcatctc attcaaccgg gtggtgcgct gttggacgag
     1861 ccgttcaaga tggagcgcgc ggctggcaag ctgttcatgc gcttcccgca aagcttcttc
     1921 cgcgcgtttt tgttccgtga cctcaagggc catcgcgccg atgccataca ccttgccatt
     1981 cggatggcgc acgggatagt agctggcaat ccaatgccgc tgttcaccgg gcttggccgg
     2041 ggtttcgccc gtcaactcca cgcgaagaac cggctgcccg gtatcacgga cctgccgtag
     2101 atattcttcg acccgttcgc caacgccggg gaacagttcc cccggtcttt taccgaagtg
     2161 tgcatctttt ggaagaccat tgatctccgc aaggcggtcg ttcgcggaaa taaatcgcaa
     2221 ctcagaatcg aatagcgcga tggcaatcgg cgcgtattcg accaggcttt cgaaacgggc
     2281 aagcaggtcc tgcaacttct cctccgcctg cttccgttcg gtgaaatcac ggaacaccag
     2341 caccacgccg tacaacggac cgctcgcttg ctggatgggc gcggcgctgt cgtcaatcgg
     2401 caattccgtc ccgtcctttg cgacaagcac agtatggttc gccaagccga ccacgctgcc
     2461 caatcgcagg actttttcca cgggattttc cacggggtgc cgcgtctttt cgttgatgat
     2521 gcggaagatt tcgggcaacg gtttgtccac agcgtcggat gtggcccatt tcgtgagccg
     2581 ttccgcttcg ccgttgagaa atgcgacgtg cccctgggca tcggtcacga tgaccgcgtc
     2641 gccgatgctc gcaagtgtcg tggtcatcaa ttctttttcc cggtacaagg cttcgcgcgc
     2701 cgcgacaagg tccgtgatgt catgaacgat cttggaagcc ccgatgacgt gtccttccgg
     2761 gtctttgatc ggtgaaatgc tcgccaaaac ggggatatgc cggccatcct tggccacacg
     2821 aacagtttca aaacgctcga ccgggcgacc ttgccggaga ttatcaagga tgtggtcctc
     2881 ctccttgagc cggtcttgcg gaaacaaagt ggtcaccggc ttgccgacga tttcttccgg
     2941 acgatagccg aacaaccgct cggcgcccgc attccatgac cgcacaatgc ctttgaggtc
     3001 ctttgcgata attgcgtcgc cggaataaga cacaatcgcc gcaagacgcg catcggcttc
     3061 ctgcgctttc ttgcgctctg tgaaatctcg gaacacaagc acaatgccga acatcgggcc
     3121 gcccggttcg cggatgggcg cggcgctgtc atcaatcggg atttcgcggc catccttcgt
     3181 cagcaaaatc gtatggttgg caaggccgac caccgagcca aggcgaacca ccttttcaac
     3241 aggattctcg acggtctggc gcgtgtgttc gttggcaatg cggaacactt cgggcaaagg
     3301 ccggcccagc gcctcggcgc ttgtccacgc cgtcaaacgt tccgcctcgg cattcaggaa
     3361 ggtgacgcgt ccctgcgcgt cggtcaaaat caccgcgtca ccgatgcttt ttaagctcgt
     3421 ggtcagcagt tccctttgac gcgcttcggc ctgttgcgcc agct
//
LOCUS       DASZMM010000573         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3808841, whole genome shotgun sequence.
ACCESSION   DASZMM010000573 DASZMM010000000
VERSION     DASZMM010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808841"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VG754_13775"
     CDS             <1..492
                     /locus_tag="VG754_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF20334.1"
                     /translation="LPLIYLFGGLFTVFTMNWIGRWADRAGKLKVFVWMSLSTAVPIL
                     LITNLPRVPVVVAIAVSTLLMICMTGRMVPAMALMTGAVEARYRGGFMSINSSVQQFS
                     MGLTGLFSSMIIGTGPHGEMTHFAINGIVSIACAYACVYLARFLKTPAHGEHVAEPMA
                     MEA"
     gene            complement(505..783)
                     /locus_tag="VG754_13780"
     CDS             complement(505..783)
                     /locus_tag="VG754_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20335.1"
                     /translation="MKNLTSPFLIKFKGIVFLLVGIASGALLILELPTIRVAVLLAIT
                     IWGFCRSIIARFTSSNITSIQATASPAFGPLPVIFGNDGARKKFEHKV"
     gene            complement(998..2284)
                     /locus_tag="VG754_13785"
     CDS             complement(998..2284)
                     /locus_tag="VG754_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20336.1"
                     /translation="MELIVPPTTHHWELFSRIGYLYQSQAMVKGEIFSPYLGLAGLAG
                     LIWIMVEALQAMMRRGPRRVPVQALQCVWVLLYATLGGMNCLLGLAGIMFFRASNRYS
                     VIIATLALLFLAMRLSRLSLRWPRHVSWVAAGVVAMVALTDQLPVKTEAAETTMLREH
                     VNNDERLAKEMEAKLPVGAAVFQMPAMVFPEALPIGQLLGYEMFAPYVYTKHLRFSFG
                     STRGRTREEWQWEVEKMPVPQMIESLEKFGFSAIYINRKAYADHADDLLKKLGEAGCK
                     IVCEDDMHDLICVELKPSSSPELPHTDDRAQIVLKKGWIIHERTPLENQQWSDGDASL
                     TFFTDQKQVTSYSLKCGIGSISLRHVSIEIKGHEVWSGEIPAGKAVPVVLTLDGWPGN
                     NTIQFKTDQPPVKPNDSAPPLAFTVINLQIIRELPH"
     gene            2348..2977
                     /locus_tag="VG754_13790"
     CDS             2348..2977
                     /locus_tag="VG754_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20337.1"
                     /translation="MNPAVSQRIEVDCEKRDQGQPKASETDLEILLSPTKNPLEQNAE
                     KQELKKHPKRVRKAQPHHQRSDDAYPAASIKPFHPPAERNQPRGQQGERHCIDEGPKK
                     LAAHVAKKLVINKSKDERPGERPFPTAPEKTGACIKRYRSRGMAKPNCRRISRNHTNP
                     PGQPSNQPDDDFFIHRVIIPVGLIRRAQALNCAILEWPEVSLLRGLYPT"
BASE COUNT          850 a          920 c          886 g          761 t
ORIGIN      
        1 ttgccgctga tctatctgtt cggcggtttg tttaccgtgt tcacgatgaa ctggatcgga
       61 cgttgggcgg accgcgcggg caaattgaag gtgttcgtct ggatgtcgct ttcgactgcg
      121 gtgccgattt tgttgataac aaacctgccg cgtgtgccgg tggttgttgc cattgcggtt
      181 tcgacgttgc tgatgatttg catgacggga cggatggtgc cggcgatggc attgatgacg
      241 ggcgcggttg aagcgcggta tcgcggcgga ttcatgagca tcaattcctc ggtgcagcaa
      301 ttttcgatgg gcctgaccgg actgttcagc tcgatgatca tcggcaccgg gccgcacggc
      361 gagatgacgc attttgcgat caatggaatt gtttccatcg catgcgccta cgcgtgcgtt
      421 tatcttgcgc gtttcctgaa aacgcccgcg catggcgaac acgtcgccga accgatggca
      481 atggaggcgt gaacaagctc agatttaaac cttatgctca aactttttcc ttgcgccgtc
      541 gttgccaaag ataacgggca aaggaccaaa ggccggagaa gcggtagctt ggatcgacgt
      601 aatgttcgat gacgtaaaac gcgcaataat agagcggcag aaaccccaga tggttatcgc
      661 gagcagaacg gcgaccctga tggtcggcaa ttccagaatc aacagcgcgc cggaggcgat
      721 gccgaccagc aaaaacacaa tgcccttgaa cttgatgaga aaaggactgg tgagattttt
      781 catgcgaaag ctcccgcagt ttgttcgcaa ccatgcgcag ggacaagttg aaagcatcgg
      841 cgatttgaga ttagaaaatg ctctccgccg aaaaactgct tgcactttcc accgcaaccg
      901 ctggctgcgc gcgccacata tagacatgaa ggccggtttt ccaacagtct tgataagacg
      961 gttctgcctc ctggcaaact cttcccggca tcgtttttca atgaggcaac tcgcgtatga
     1021 tttggagatt gatcacggtg aaggcaagcg gcggcgcgga atcgtttggc ttaaccggcg
     1081 gctggtcagt tttaaattgg atcgtgttat tgcccggcca gccatcaagc gtaagaacga
     1141 ccggcacagc tttgccagca gggatttcgc cgctccacac ttcatgccct ttgatctcga
     1201 tgcttacatg acgcagggaa atgctgccga tgccacattt gagcgagtaa ctggtcacct
     1261 gcttttggtc ggtgaaaaaa gtcagcgacg cgtcgccgtc ggaccattgc tgattttcaa
     1321 gtggcgtccg ctcgtggatg atccagcctt ttttcaacac gatttgcgcg cgatcatccg
     1381 tgtgcggcag ttccggagaa gaagacggct tgagctccac gcaaatcaaa tcgtgcatgt
     1441 catcttcaca aacgatttta cagccggctt cgcccagttt cttgaggaga tcatccgcgt
     1501 ggtctgcata ggctttgcgg ttgatgtaaa tcgcggagaa gccaaatttt tccaaggatt
     1561 cgatcatttg cgggacaggc attttctcca cttcccattg ccactcttcc cgcgtgcggc
     1621 cgcgcgttga accgaaagaa aaccgcagat gttttgtata gacgtacgga gcgaacattt
     1681 cgtaacccaa aagctgcccg atgggcaggg cctcgggaaa gaccatggct ggcatttgaa
     1741 ataccgccgc cccaaccggg agcttggctt ccatttcttt tgcaagccgc tcgtcgttgt
     1801 tgacgtgctc gcggagcatt gtggtttccg ccgcttcggt ttttacgggc aattgatctg
     1861 tcaaagccac catggccacc acgcccgccg cgacccaact gacgtggcgc ggccatcgca
     1921 agctcagccg cgacaaccgc atggccagga acagcagcgc gagcgtggca atgatgacgc
     1981 tataacggtt gctggcgcgg aaaaacatta tcccggccag accgagcagg caattcatcc
     2041 cgcccagcgt cgcgtaaagc aaaacccaca cgcattgaag cgcctgcacc ggaactcggc
     2101 gcggcccgcg ccgcatcatc gcttgcaacg cctcgaccat gatccaaatc aacccggcga
     2161 ggcccgccaa tcccaaatag ggactgaata tttcgccctt caccatggct tgcgattgat
     2221 acaggtagcc aattctgctg aacaactccc aatgatgcgt cgtgggcggc acaatcagtt
     2281 ccatcggttt cagcgagtat atttctgtct cgagataatg acgggacatc ccctcggaat
     2341 ttttgccatg aacccagccg taagccagcg tattgaggtg gattgcgaaa aacgcgacca
     2401 gggccagccc aaagcaagcg aaaccgatct ggagattctc ctttcgccga cgaagaatcc
     2461 actggaacaa aatgctgaaa aacaggaact gaagaaacat ccaaagcgtg tacggaaagc
     2521 tcaaccccat caccagcgaa gtgacgatgc atatccagca gcttcgatca aaccatttca
     2581 tcctcctgct gaacgaaacc agccacgtgg tcagcagggc gaacggcact gtatagatga
     2641 aggtccgaaa aagctggccg cccacgttgc aaaaaagctg gtgataaata aaagcaaaga
     2701 tgagcgcccc ggcgaacgcc cattcccgac ggcaccggaa aaaacgggcg catgcataaa
     2761 aagatatcgc agccgtggca tggcaaagcc aaattgccgc cgtataagcc ggaatcacac
     2821 caatccaccg ggccagcctt ccaaccagcc agatgatgat tttttcatac atcgggtaat
     2881 cattccagtt ggcttgataa ggcgcgccca agcgctgaac tgtgcaattt tggaatggcc
     2941 cgaagtctcc ctcctgcgcg gcctttatcc aacctagtat caaaacatca tcacccctat
     3001 aagttggcgg cacgttccag ttgctgaaat gtaaacggtc cctggtatga ccccaaataa
     3061 aaaatgtggt tatgcacaag agcagtatta ataccagatc caagtacgtt ttgcttgatt
     3121 ttgaaccaaa tgacggcatc tgactctctt atcatgtcac caaccgagga agcaattaga
     3181 aaaatattct gagacaacac atttcccgga gggtccggga aggcctgcag cctttccaac
     3241 ggcaatgaga ttatcacagg ctttaatcaa ggcagaaaat atcgcgtttg ttaagtggcc
     3301 tcatttcctg tcgtcaggaa aacgctgcaa attggcgacc gtattggata tcacgtcata
     3361 ttctttaatc ccttccggcg acttgaaaat aatggtctga ttgccactct gcgcctt
//
LOCUS       DASZMM010000574         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4112082, whole genome shotgun sequence.
ACCESSION   DASZMM010000574 DASZMM010000000
VERSION     DASZMM010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4112082"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VG754_13795"
     CDS             complement(<1..885)
                     /locus_tag="VG754_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF20338.1"
                     /translation="MARDFHIAILSDVHYAGAEERMRGNDSEYRDLPNPLLRTLNWAY
                     RHFLWMRHPLDHNTQLDEFIRRAGSPDWVIANGDYNCDTHFVGVSDDASFQSAHECLA
                     KLRDRFPTNLRATFGDHELGKMSTLGGRGGIRLASFRRAREELGLEPLWRLDLGNRVL
                     LGVTSTLVALPMFEVDALPEERAEWRQLREEHLARIRETFKGLKPAQRVMLFCHDPSA
                     LPFLWREEIVRGKIAQLEQTIIGHLHSNLIYFKSRLLAGMPRITFLGHTVKRLSTALS
                     EGRHWRPFKVRLCPSLAGI"
     gene            complement(895..>2147)
                     /locus_tag="VG754_13800"
     CDS             complement(895..>2147)
                     /locus_tag="VG754_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF20339.1"
                     /translation="PLRSSDKLLFPNSLAMPELAVTLATLLGVADSGARPVKPISQQQ
                     MTRCTMPTDVPVPKTEGPNDGLDFVSVLRDCLSCGVIVLDERQKVTGFNPRAEQLTGL
                     KSATMAGHGSELLPAPLESLLKDAFSSSEPVSHRQVMLRNAKGADFAVLASANSVLTA
                     DGKVSGCVLVLNDVSAIRKWEGNMRRLDRLHSVGTLSASMAHEVKNAFVAVRTFVDLL
                     LEKNKEMELASVVRQELNRIDSMVKQMLKFSGPPKPELTSVHVHLVLDKSLLLIRHLL
                     EEKKIRLKRSFTAASDVMNGDQDQLEQAFLNLFFNALDAMDSNGNLEVSTTLLPPGAK
                     IEGLPVAAGQRYLNVIIRDSGIGIPSENMGRLFEPFFTTKPEGTGLGLAITRRIIQEH
                     QGIISVQSELNKGTAFSLVFPARV"
BASE COUNT          500 a          631 c          568 g          448 t
ORIGIN      
        1 aattcccgcc agcgaaggac acaatcgcac tttgaacggc cgccaatgcc gtccttctga
       61 aagcgccgtg ctgagacgtt tgacggtgtg gccgagaaaa gtgattctcg gcattccagc
      121 cagcaaacgg ctcttgaaat aaatcaggtt cgaatgcaga tgcccgatga ttgtctgctc
      181 aagctgggca attttgccgc gcacgatttc ttcacgccat agaaacggca gcgcggatgg
      241 gtcatggcaa aagagcatca cgcgttgcgc cggcttcaga cccttgaacg tctcgcgaat
      301 ccgcgccaga tgttcttcgc gcaattgccg ccattcggcg cgttcttccg gaagcgcgtc
      361 cacctcgaac atcggcaacg ccaccagcgt tgaagtgacg cccagcaaaa cgcgattgcc
      421 caagtccaaa cgccaaagtg gttccaagcc gagttcctca cgcgcgcgac gaaaacttgc
      481 cagacggatg ccgccacgtc cgccgagcgt actcatcttg cccaattcat gatcgccgaa
      541 cgtcgcgcga aggttcgtcg gaaaacggtc gcgcaatttt gccaaacatt catgcgcgct
      601 ttgaaacgaa gcatcgtcgc ttacgccgac gaaatgggtg tcgcaattgt aatcgccgtt
      661 ggcgatcacc caatcaggcg aaccggcgcg gcggatgaac tcgtccaatt gcgtgttgtg
      721 gtcgagcgga tgccgcatcc agaggaaatg acgataggcc caatttaagg tgcgcagcaa
      781 cggattcggc aggtcgcgat attcggaatc gttgcctcgc atccgttctt cggcgccggc
      841 gtaatggaca tcactgagga tggcaatgtg aaaatcgcgg gccatgcttg ggcatcaaac
      901 gcgcgcggga aaaaccaggc tgaatgccgt gcccttgttc aactcgcttt gcaccgaaat
      961 gattccttga tgctcctgga tgatccgccg cgtgatggcc agccccaatc ctgtcccttc
     1021 cggcttggtc gtgaaaaacg gctcaaacaa tcgtcccatg ttttccgaag gaatgccgat
     1081 gccgctgtcg cggattatca cgttgagata acgctgccca gccgccacag gcaaaccctc
     1141 aatttttgcg ccgggcggca gcaacgtggt ggaaacctcc agattgccgt tggaatccat
     1201 cgcatcgagc gcattgaaaa aaagattcag gaaagcctgc tccaactggt cctggtcgcc
     1261 attcatcaca tccgaagcgg cggtaaacga acgcttcagc cggattttct tttcctcgag
     1321 caaatgcctg atcaacagca gcgacttgtc gagaacgaga tgcacgtgga cagaagtcag
     1381 ctccggtttg ggcggccccg aaaatttcag catctgcttc accatggaat caatgcggtt
     1441 caattcctgg cgaacgacgc tcgcaagctc catttccttg ttcttttcca aaagcaggtc
     1501 cacaaacgtg cgcaccgcga caaacgcatt cttcacttca tgcgccatgc ttgccgaaag
     1561 cgtgccgacg ctgtgcagtc gatcaagacg gcgcatgttt ccctcccatt tccggatggc
     1621 ggaaacatca tttaacacca gcacgcaacc ggaaactttt ccatcagccg tcaggacaga
     1681 attggctgag gccagcacgg caaaatccgc gcctttcgca ttgcgaagca tcacctgacg
     1741 atgacttaca ggctcgctgc tggagaaagc gtccttgaga agagattcca atggcgcggg
     1801 aagtaactcc gatccatggc cggccatcgt tgcgcttttg aggccggtga gttgttccgc
     1861 gcgcgggttg aatccggtca ccttctggcg ttcgtccaaa acaatcaccc cgcacgacag
     1921 gcaatcgcgc aaaacggaga caaaatccag cccgtcattt gggccttctg ttttcggaac
     1981 tggaacatct gtcggcatag tacaccgggt catctgctgc tgactgattg gcttcaccgg
     2041 ccgcgcgcct gaatccgcaa caccgagtaa tgtcgccagt gtaactgcaa gttccggcat
     2101 tgccaagctg tttgggaaca gcaacttgtc tgacgaccgg agcggaa
//
LOCUS       DASZMM010000575         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4929640, whole genome shotgun sequence.
ACCESSION   DASZMM010000575 DASZMM010000000
VERSION     DASZMM010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4929640"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VG754_13805"
     CDS             <1..671
                     /locus_tag="VG754_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20340.1"
                     /translation="AFLSSGFFLAAAMHFARPEVSPLEQPLQVRGFHVAREVLFALGF
                     LLVVLLLSEPFLAQESQKAELPLRLHLPTVGSIVSAGKTHNRPFIMNQSILTLLLFFV
                     LQGLLYIGSLVKLAEIRRQNAPTRVKLKLLENEEHLFDAGLYLGFVGTIISFILHSMG
                     ITELSLLGAYSSTSFGIIFVSVFKIFQLRPARRKLLLEAEADAASGEITAAPVVAPAL
                     APMP"
     gene            668..2110
                     /locus_tag="VG754_13810"
     CDS             668..2110
                     /locus_tag="VG754_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20341.1"
                     /translation="MNRSILIVICDFLLVSLLAFSTVDNTGKLSDKAGKLQMQTGVAT
                     NRVEARQDLGSAMQLALAEERKSRDQLLGELTKMRDASVRQQTTLSEREKQVQSYQQQ
                     LQTHQQEEQQLQNQQAALQKQFAAAQTNIQTLNQKLLNASNENVLSKESIAATEAEAR
                     RQAEDATALRRKLEQLNKDSQQMAAEKQKLASELQVAEAEKRSAAELAKRMEEEVKVE
                     REEKAKLAEGVKVLANKSGELVQEIRENRPLAPNTIFQDFISNRVEARFEATRSTLFG
                     IDSDRRRDTQTILVGDGTNLFALCHVQDTPLTLWSPGTEWEGLTGNLNRNATSVSIHS
                     LSFYMLDPRVVLMPVSAADARALGGKIYRLSSDPFKFQDAVVVGTRESYYGEVKFELD
                     VSTPQYVKMDRNSLKGLFGKFNPSRGDLVFSRNGDLLGVMANGTYCMLIHNFDADPTI
                     RFGPDVRAQHTGSTLSMLASTVMQKPFKLQ"
     gene            complement(2142..>2718)
                     /locus_tag="VG754_13815"
     CDS             complement(2142..>2718)
                     /locus_tag="VG754_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20342.1"
                     /translation="PCRRPLVVIQCASASEPFLKALSGTNRVVITATRSGYELNVTRF
                     GSFMARAIADPAADLDKDGQTSLLEAYLMASRQVEQFYKDQGRLLTEHALLDDNGDGL
                     GTPPDWFNGLRAVKKAADGKSVDGVRANQMILVRGENEQKLSPEVRARRDELEQKLSA
                     LRARKSQMKEDDYFREVEAIMVETARLYQNK"
BASE COUNT          597 a          825 c          720 g          576 t
ORIGIN      
        1 tcgcatttct ttccagcgga ttttttcttg ccgccgcgat gcattttgcg cggccggagg
       61 tttcgccgct ggaacaaccg ttgcaagttc gcggcttcca tgtcgcgcga gaagttttgt
      121 ttgccctggg tttcctgctc gtggtacttc tcctgagtga gccgtttctc gcccaggaaa
      181 gccagaaagc ggaattgccg cttcgactgc atctgcccac ggtgggcagc atcgtctccg
      241 ctggaaagac ccacaaccgc ccattcatta tgaaccaaag catattgacc ttgctgctgt
      301 ttttcgtgct gcaaggcctg ctctacatcg gctcgctcgt caagctcgcc gagatccgac
      361 gccaaaacgc gccaacgcgc gtcaagctca agctgctcga aaacgaggaa caccttttcg
      421 atgccggcct ttacctcggc tttgtcggca cgatcatctc cttcatcctg cactcgatgg
      481 ggatcacgga gttgagcctg ctcggcgcgt actcgtccac atcgttcggc atcatctttg
      541 tttccgtctt caagattttc cagttgcgcc ccgcgcgacg gaaattgctg ctggaagcgg
      601 aagctgatgc tgcatccggt gaaataacag ccgcgcccgt cgtcgcgccg gcgctggccc
      661 cgatgccatg aaccgctcca tcctcatcgt catctgcgat ttcctcctgg tgagcctgct
      721 ggctttttcc acggtggaca acaccggcaa actgtccgat aaggccggca agctgcagat
      781 gcagacgggt gtcgcaacga accgcgttga ggcacggcag gatttgggaa gcgcgatgca
      841 actcgccctt gccgaggagc gcaaaagccg cgaccagttg ctcggcgaac ttaccaagat
      901 gcgcgacgcg tccgtgcgcc agcaaaccac gttgagtgaa cgagagaaac aggtccaatc
      961 ctatcaacag caattgcaga cgcaccagca ggaggaacaa cagttgcaga accagcaggc
     1021 ggcgcttcag aaacaattcg ccgccgcgca gaccaacata cagacgctga accagaaatt
     1081 gctcaacgct tccaacgaaa atgtgctgtc aaaagaatcg attgccgcca ctgaagctga
     1141 agcgcggcgt caggccgaag atgctacagc actgcgccgg aagctggagc aactcaacaa
     1201 ggacagccag caaatggccg cggaaaagca aaagctcgcc agtgaattgc aggttgccga
     1261 ggctgagaaa cgttcggcag ccgaactcgc caaacggatg gaagaggaag tgaaggtgga
     1321 gcgtgaggaa aaagccaagc tcgccgaagg cgtcaaggtg cttgccaaca agtcgggcga
     1381 actcgtgcag gaaatccgcg agaaccgtcc gctcgcgccc aacacaattt ttcaagactt
     1441 tatcagcaac cgcgtcgagg cccgttttga agccacgcgc tcgacacttt tcggcattga
     1501 ttcagaccgc cgccgcgaca cgcaaaccat cctcgtcggc gatggcacca acctttttgc
     1561 gctttgccat gtgcaggaca ccccgctcac gctttggagt cccggcacgg aatgggaagg
     1621 cctcactgga aatctgaatc gcaacgccac ctcggtttcc atccattcgc tttcgtttta
     1681 catgctcgac ccgcgggtcg ttttgatgcc cgtcagcgct gccgacgcgc gcgcgctggg
     1741 cggcaaaatt tatcgtttgt ccagcgaccc gttcaaattc caggacgcgg tcgtcgtcgg
     1801 cacgcgtgaa agttattacg gtgaagtcaa atttgaactc gacgtgtcca cgccgcaata
     1861 cgtcaagatg gaccgcaatt cattgaaagg tttgttcggc aaattcaatc cctcgcgcgg
     1921 cgatttggtg ttcagccgca acggcgatct tctcggtgtg atggccaacg gcacttactg
     1981 catgctcatc cacaatttcg atgccgatcc caccatccgt ttcggtccgg acgtccgcgc
     2041 gcaacacacc ggttccacac tctccatgct ggcttcgacg gtgatgcaga aaccgtttaa
     2101 attgcagtaa tcgagacgcg tgaaattttt cccactcgca tttacttgtt ctgataaagc
     2161 cgcgctgttt cgaccatgat ggcttccact tcgcggaaat aatcgtcttc tttcatctgt
     2221 gacttgcggg cgcgaagggc gctcagcttt tgttccaatt catcgcggcg cgcgcggact
     2281 tccggggaca gcttttgctc gttttcaccg cgaaccaaaa tcatttggtt cgcgcgcacg
     2341 ccatcaacgg attttccgtc cgccgccttt ttgacagccc gcaatccgtt gaaccaatcg
     2401 ggcggcgtgc cgagcccgtc gccattgtca tccaacaaag cgtgttcggt caacaagcgg
     2461 ccctggtcct tatagaattg ttccacctgc cgcgacgcca tcaaataagc ttcgagcaaa
     2521 gaagtctgac catccttgtc caaatcagcc gcaggatcgg caatcgcgcg cgccatgaag
     2581 ctgccgaatc gggtcacatt gagttcatac ccgctccgtg tcgcagtgat gacgacccgg
     2641 ttcgtgccgg aaagcgcctt gagaaacggc tcgctcgccg acgcgcattg aatgacaacg
     2701 agcggacgac gacacggt
//
LOCUS       DASZMM010000576         3740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2177353, whole genome shotgun sequence.
ACCESSION   DASZMM010000576 DASZMM010000000
VERSION     DASZMM010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177353"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VG754_13820"
     CDS             complement(<1..735)
                     /locus_tag="VG754_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20343.1"
                     /translation="MENSCSHLTIAENLPMDKINQTVVVTGISNREFLERYARAGRVG
                     LSGGITLIDKAIARAERHLDPAGTWGSWSHSFLFQGTRHDGHHWVIESDLQVNRKHIR
                     LGVQENRISKYYDEDFYTTLAVLDFDLPEATVTNLLGEGLELVAQRARYSMRELVGTL
                     IALRDSKLRAKENVLAQQSSMYCSAFIQHLFHKTGIDLAPGVHRSNTTPEDISRTAVP
                     HVTYLLHRDPPPKKLAELRRRMRERVQ"
     gene            complement(821..2569)
                     /locus_tag="VG754_13825"
     CDS             complement(821..2569)
                     /locus_tag="VG754_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF20344.1"
                     /translation="MAFAFPYRRAVLGILGITLLLAAANAVEPLVLKSIFDNLAGNRI
                     VRGLVTSICILIGIGLFREAATAITNWMTWHTRLGLHHALLESTVERLHRMPLGFHRT
                     EGVGAIMTRLDRSIQGFITAVSQILFNVFPAVLYLAIALVIMLRLNWKLALIVAAFLP
                     LPGIIAALGGPEQTRRERTLLDQWSKIYSRFNEVLSGIVTVRSFSMEDTEKRRFLKHV
                     HEANQVVIRGVGIDSGLGAATNLAVTAARITAIAFGGMLILQGHLTLGTLVAFLGYVG
                     GLFGPVQGLTTIYQTIQRAYVSLDEIFSILDVQEHLGDAPDATEVAGVRGEVVIRGLK
                     FRYDPNSRPLLQGIDLHVKPGETVAIVGPSGSGKTTLMALLMRFYDPDEGSIFLDGRD
                     LRTLKQRSLRHNIGTVLQDPLLFNDTVRNNIAYGRPNASIAEVEAAARAANAHDFILH
                     MPESYETMVGERGGRLSLGERQRVTIARALLKNPPILVLDEATSSLDAESESLVQEAL
                     ERLMKGRTTFVIAHRLATVVNADRIIVLKHGRIIESGTHWELMRADGYYASLVTRQTN
                     GLINNPVAKRREIAAY"
     gene            complement(3033..>3740)
                     /locus_tag="VG754_13830"
     CDS             complement(3033..>3740)
                     /locus_tag="VG754_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HWF20345.1"
                     /translation="VRELGRWFHNLDLDGVQTAPDHFLGDYPRLKWQTFADAIPQDLE
                     GRSVLDIGCNAGFYSLEMKRRGAGRVVGIDSDPAYLAQARFAAEVSGFEDDVELRQLS
                     VYDVAQLGEKFDIVLFMGVLYHLRHPLLALDLIHEHVARDLLVFQTMQRGSNAVENVA
                     ENPSFHEKDIFDQAGFPRMYFIEKKYANDPTNWWIPNRACSEAMLRSAGFEILEHPEE
                     EVYICKRQQVRDFKEGL"
BASE COUNT          846 a         1069 c         1018 g          807 t
ORIGIN      
        1 ctggacgcgc tcgcgcatcc ggcggcgcaa ctcggcaagc tttttcggcg gcggatcgcg
       61 atgcagcaga taggtcacgt gcggcacggc ggtgcgcgaa atgtcctccg gagtggtgtt
      121 gctgcgatgc actcccggcg caaggtcaat gcccgtttta tgaaataaat gctggatgaa
      181 cgccgagcaa tacatggagc tttgttgggc aaggacattt tcttttgcgc gcaatttgga
      241 atcgcgcaac gcgatcaacg tgcccaccaa ttcgcgcatc gagtatcgcg cccgttgcgc
      301 gaccagttcc aatccttcac ccaggagatt tgtcactgtg gcttccggca aatcaaaatc
      361 cagcaccgcg agcgtcgtgt aaaaatcctc gtcgtaatat tttgagatgc ggttttcctg
      421 cacacccaac cggatgtgct tgcggttcac ctgcaagtcg gactcgatca cccaatgatg
      481 tccatcgtgc cgcgtgcctt gaaataaaaa tgaatgcgac cacgaacccc acgtgccggc
      541 aggatcaaga tgccgttcgg cgcgggcgat ggccttgtca atcagcgtga tgccgcccga
      601 aagcccgaca cgtcctgcgc gcgcataccg ctcaagaaac tcgcggttcg agatgcccgt
      661 caccaccacc gtttgattga ttttgtccat cggcaggttc tcggcgatgg taagatgcga
      721 acaagagttt tccaatacaa ttgtgtagga ggcgacgtga ggctctcttg ggatttcaaa
      781 tttgaaattc caaatctcag attaaacgtt tgacaaagga ttaatacgcc gcaatttccc
      841 tgcgcttcgc gacgggatta ttgatcaatc cattcgtctg gcgcgtcacg agcgaagcgt
      901 aatatccatc cgcgcgcatc aattcccagt gcgtgccgct ttcgatgatg cgcccgtgct
      961 tcagcacaat gatgcggtcg gcattcacaa cagtcgccaa acgatgcgca atcacaaatg
     1021 tggtccgtcc tttcatcaag cgttccagtg cctcttggac caacgattcg gattcggcat
     1081 cgagagacga cgtggcttcg tcgaggacga gaatcggcgg atttttgagc agcgcgcggg
     1141 cgatggtgac tcgctggcgt tcgcccaatg aaagccttcc gccgcgttcg ccgaccatcg
     1201 tttcgtaact ttccggcatg tgtagaatga aatcatgcgc atttgcggcg cgcgcggcgg
     1261 cttcgacttc cgcaatcgaa gcattcggac ggccataagc gatgttgttg cgcacggtgt
     1321 cgttgaagag caacgggtcc tgcaaaacgg tcccgatgtt gtgccgtaac gaacgctgct
     1381 tcaacgtcct caaatcgcgt ccgtcgagaa aaatgcttcc ttcgtccgga tcataaaacc
     1441 gcatcaacaa cgccatcagg gtcgtcttgc ccgaaccgct cggcccgaca atggcaaccg
     1501 tttcgcccgg cttcacatgc agatcaattc cctgcaacaa cggccgcgaa ttcgggtcgt
     1561 aacggaattt caacccgcgt atcacaacct cgccgcggac accggccact tcggttgcat
     1621 ccggcgcgtc gccgagatgt tcctgcacgt cgaggatgga aaaaatttcg tcgagcgaaa
     1681 catacgcgcg ctggatggtc tgataaatcg tggtcaaacc ttgcaccggc ccgaataatc
     1741 cccccacata accgagaaac gccaccagcg ttcccagcgt gagatgtcct tgcaagatca
     1801 acatcccgcc aaacgcaatc gccgtgatgc gcgctgccgt gacggccagg ttcgtcgcag
     1861 cgccaagacc ggaatcaatt cctacaccgc gaatgaccac ttggttcgct tcatgcacat
     1921 gcttgagaaa acggcgcttc tcggtgtcct ccatcgaaaa gctgcggacg gtgacgatgc
     1981 cggaaagcac ttcattgaag cgcgaataga ttttcgacca ttgatcaagc agcgtgcgtt
     2041 cgcggcgtgt ttgttctggg ccgccaagcg ccgcgatgat tccgggcaac ggtaaaaatg
     2101 cggcgacgat cagcgccagt ttccaattca gccgcaacat gatgaccagc gcgatggcga
     2161 gataaagcac cgccgggaaa acgttgaaca aaatctggct cactgcggtt atgaatccct
     2221 ggatgctgcg gtccaaccgc gtcatgatcg cccccacccc ttcggtgcga tggaaaccga
     2281 gcggcatccg gtgcagccgt tcgacggtgg attcgagcag cgcatgatgc aagcccagcc
     2341 gcgtgtgcca tgtcatccag ttggtgatgg ctgtggcggc ttcgcggaac agaccgatgc
     2401 ctatcaagat gcagatgctg gtgacaaggc ctcgcacaat gcgattgccc gcgagattgt
     2461 cgaagatgct tttgaggacg agcggttcaa cggcgttggc tgccgcaagc aggagcgtta
     2521 tgccgagaat gccgaggacg gcccggcgat aagggaaagc aaaagccatc gcgcgtcgca
     2581 gcctgcccga gctgcccgcg cgaaccgttt cggttttggg ttcataatct gacaaatgag
     2641 aagattcgac cccacgaccg cgcgagccgt tcgatatccg cgaaattttc ttgcctgcaa
     2701 aaaacgcccg tgatactctc gtttccgttg gaaggaggcg ggcaagttct ttgacatcct
     2761 aaaatggtta atggctgaga gttgaggtgc ttccgcgcgg gccatggctc cgcatccagc
     2821 atcgcgccaa gttgtaagtg gttgtaaacc cgcttaaacc cgcatagctt cgcctaaacc
     2881 cgctaaaaaa cggcacccca ccggtgtgaa gttcaaggtt gaaggtttaa tgttcaaagt
     2941 tggcatcctg ccatcctcta tcatctatcc cccatcctcg ggtttcaagt ttgtcgctta
     3001 atgtcgcttc aggtctcaag ggcaacgtca aactataaac cttctttgaa gtccctgact
     3061 tgctgacgtt tgcagatgta aacttcctct tcggggtgtt cgaggatttc gaagccggcg
     3121 ctgcgcaaca tggcctcgga acaggcgcgg ttgggtatcc accaattggt cgggtcgttc
     3181 gcgtattttt tctcgatgaa atacatgcgc ggaaaacccg cttggtcgaa gatgtccttc
     3241 tcatggaacg aaggattttc tgccacattt tccacggcgt tgctgccccg ttgcatggtc
     3301 tggaagacaa gcaaatcgcg cgcaacgtgc tcatggatga ggtcgagcgc caacaatgga
     3361 tgccgcaaat gataaagcac acccatgaac agcacgatgt cgaacttttc accgagttgc
     3421 gcgacgtcat agaccgaaag ctggcgcagt tcaacgtcgt cctcgaagcc ggaaacttcc
     3481 gcggcaaaac gcgcctgcgc caagtacgcc gggtcggaat caatccccac gacacgtccc
     3541 gcgccgcgtc gcttcatttc gagcgagtaa aatcctgcgt tgcaaccgat gtccaagacc
     3601 gacctgcctt cgaggtcttg gggaatggca tcggcgaacg tttgccattt gaggcgcgga
     3661 taatcgccga ggaaatggtc cggcgcagtt tgcacgccat cgaggtcaag gttgtgaaac
     3721 catcggccaa gttcgcggac
//
LOCUS       DASZMM010000577         3782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3554424, whole genome shotgun sequence.
ACCESSION   DASZMM010000577 DASZMM010000000
VERSION     DASZMM010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554424"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VG754_13835"
     CDS             <1..193
                     /locus_tag="VG754_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20346.1"
                     /translation="FVTWWAHSKWGFPYTPLMIVITSLMSALAIYKHKANIVRLMNGT
                     ENKIGRKKETAPPQTKAAP"
     gene            190..1170
                     /locus_tag="VG754_13840"
     CDS             190..1170
                     /locus_tag="VG754_13840"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWF20347.1"
                     /translation="MNVTVLGAGAWGTALAKLLHKNGNTITLWGHDAQRLEEVRRTRC
                     NERYLPGIELPRDWRIESDLARAIAGSDCVVAAVPSKAFREVSRHLADFSGVAVSVTK
                     GIEDETCLTMCGVLAQTAPKARVAALSGPTFALEVAHGMPTAIVAASNDAAVANQVQN
                     LFHRTEFRVYTSTDLAGVELGGALKNVIAIAAGVCDGLGFGDNSKAALITRAMAEVRR
                     LGVICNAQAETFSGLSGLGDLTATCFSKLSRNRGFGERLGRGEKLADVLGSMITVAEG
                     YPTSRSAYQLARKQNASTPVIDEVYRMLYEGKNVAQGVRDLMSRDSKAED"
     gene            complement(1283..1888)
                     /gene="thrH"
                     /locus_tag="VG754_13845"
     CDS             complement(1283..1888)
                     /gene="thrH"
                     /locus_tag="VG754_13845"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HWF20348.1"
                     /translation="MKQTIVTLDLEGVLVPEIWIAVAEKTGIRELRLTTRDIPDYDIL
                     MKGRLNLLDQHGLKLSDIQQVISTLRPLPGGREFLDELRALTQVIILSDTFEEFAKPL
                     MKQLDWPTLFCHKLEVENDRIVNYRLRQTNQKQKSVAALKNLNYHVIAAGDSFNDTAM
                     LIEANVGFFFRAPVPIQQQFPQFKAVEAYEDLMRLMKEAMR"
     gene            complement(1960..2760)
                     /locus_tag="VG754_13850"
     CDS             complement(1960..2760)
                     /locus_tag="VG754_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HWF20349.1"
                     /translation="MSWPADYHMHTPLCHHANGEPVEYAARAVALGLTEIGFSDHSPM
                     RQDNFDNWRMNIDKLGEYVEKVRRAQKDFPQLTIRLALEVDYLPGQEDWIHELAQMHP
                     WDYFIGSVHYVSDSWAIDNPEKLSEWKKRDAFEVWSVYFDWLTRAAESGLFEIIGHAD
                     LPKKFGIYPKQDCTALFERFLSAAAKKNAAIELNTAGLRKDCKEIYPSAKIVRMAKEK
                     GVAITFGSDAHAPGEVGMNFTEAMQLARKSGYAESCRFVQRKREMVRF"
     gene            complement(2888..>3782)
                     /locus_tag="VG754_13855"
     CDS             complement(2888..>3782)
                     /locus_tag="VG754_13855"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWF20350.1"
                     /translation="MGQKSGQFIQSLMSQTQVHGKGPLATGIGIVVLILSATGVFAEL
                     QSDLNSMWGVEQKSNVGWSRMIVNRALLFVLLVAIGILLVVSLLASTAIAAMQGSVFD
                     SIPGGKQLAHGVEFLVSCGVITVLFTMIFKFLPDARPHWRDVWVGALVTAFLFTVGKV
                     LLALYLGYGSVASPYGVAGSIVLILVWVYYSAQIFLYGAEFTQVYASEHGRAIKPAKT
                     AQWKSEGEGAKEDREEAQTQGKPKKTEWTSNQPATAGSARTRSSENRGEPKHAPAHAG
                     DTVHDLADRVHGWHSLRPKRT"
BASE COUNT          846 a          997 c         1081 g          858 t
ORIGIN      
        1 gttcgtcacg tggtgggcgc actcgaaatg gggctttcct tacacgccat tgatgattgt
       61 catcacgtct ttgatgagcg cgcttgccat ctacaaacac aaggccaata ttgtccgcct
      121 catgaatggt accgaaaata agatcggccg caaaaaagaa acggccccgc cccaaaccaa
      181 agctgctcca tgaacgtcac cgtgctcggc gcgggcgcgt ggggaacggc gctcgcaaaa
      241 ttactgcaca aaaatggcaa caccatcacg ctatggggcc atgacgcgca gcggttggaa
      301 gaggtgcgtc gcacaagatg caacgagcgc tatttgccgg gaattgaact gccgcgcgat
      361 tggcggattg agtctgattt ggcgcgtgcg attgccggta gtgactgcgt ggttgcggcg
      421 gttccctcaa aggcctttcg cgaagtctcg cggcacctgg cggatttcag cggcgtggcg
      481 gtgagcgtta ccaagggaat tgaggacgaa acgtgcctga cgatgtgcgg cgtgcttgcc
      541 caaaccgccc cgaaagcccg cgtggcggcg ttgtcgggac cgacgtttgc gttggaagtc
      601 gcgcacggaa tgccgacggc gattgtcgcg gcaagcaatg atgcggcggt tgccaaccaa
      661 gtccaaaatc tttttcatcg caccgaattt cgcgtttaca cgagcacgga tttggcgggt
      721 gttgaacttg gcggcgcgtt gaagaacgtg atcgcgattg cggcgggcgt ttgtgatggg
      781 ttgggattcg gcgataattc gaaggccgcg ctcatcacgc gcgcaatggc cgaggtgcgg
      841 cggttggggg tgatttgcaa cgcgcaggcg gagacgttca gcgggttaag cgggctggga
      901 gacttgaccg cgacttgttt ttccaagttg agccgcaacc gcggtttcgg cgagcgattg
      961 ggccgcgggg aaaaattggc ggacgttttg ggcagcatga tcactgttgc ggaaggttat
     1021 ccgacgtcgc gttcagctta tcaactcgcg cgcaaacaaa acgcgagcac gccggtaatt
     1081 gatgaagttt accggatgct ttacgaggga aaaaatgtcg ctcaaggcgt gcgcgatttg
     1141 atgtcgcggg attcgaaagc ggaagactga aaataaattc caagcaccag tgaagcatca
     1201 atcattaact cgaaaatgtt tatcggtgat ttgcgcttgg tgattgaagc ttctctggaa
     1261 cttggagctt ggaaccggtg ctttacctca tcgcttcttt catcaaccgc atcaaatcct
     1321 catacgcctc gaccgccttg aactgcggga attgttgctg gatcggaacg ggagcacgaa
     1381 agaaaaatcc gacattcgcc tcaatgagca tggcggtatc gttgaaggaa tcgccggcgg
     1441 caatgacgtg gtaattgaga tttttcagcg ccgcgacgga cttttgtttt tgattggtct
     1501 ggcgcaaacg gtaattgacg atgcggtcgt tttcgacttc gagcttgtgg cagaagagcg
     1561 tgggccaatc gagttgtttc atcagcggct tggcaaactc ctcgaatgtg tccgacaaaa
     1621 tgatgacttg ggtgagcgcg cgcagctcgt ccaaaaattc cctgccgccc ggcagcggac
     1681 gcagcgtcga aatcacctgc tggatgtcgg acaatttcag gccgtgctgg tccagcagat
     1741 taagccggcc tttcatgagg atgtcgtagt ccgggatgtc gcgcgtggtg aggcgcaatt
     1801 cgcggatgcc ggttttttcc gcgacggcga tccagatttc cggcaccagc acgccttcca
     1861 agtccaatgt gacgatggtt tgtttcacag agaaagtttt ccaaaatgca gaagctgtgt
     1921 ccagtcgtac gctttgattc ggcgaattca aagtgccgat taaaatcgca ccatctcccg
     1981 ctttcgttgg acgaacctgc agctttccgc ataacccgac ttccgcgcca gttgcatcgc
     2041 ttccgtgaaa ttcatcccca cttcgcccgg cgcgtgggcg tccgagccaa acgtgatggc
     2101 cacacctttt tcctttgcca tccgcacaat tttcgcgctt gggtaaatct ccttgcaatc
     2161 cttgcgcagc ccggcggtgt tgagttcgat ggcggcgttt tttttcgcgg ctgcggacag
     2221 gaagcgttcg aacaacgcgg tgcaatcttg tttgggatag atgccgaatt ttttcggcaa
     2281 atccgcatgg ccgatgattt cgaaaaggcc cgattccgcc gcgcgcgtga gccagtcgaa
     2341 ataaaccgac cagacttcaa aggcgtcccg ctttttccat tcggaaagtt tttcgggatt
     2401 gtcgatggcc cacgagtccg agacgtaatg cacggagccg atgaaataat cccacggatg
     2461 catctgggcg agttcgtgga tccaatcttc ctgtccggga agataatcca cttcaagcgc
     2521 gagccggatg gtcaattgcg gaaaatcttt ttgcgccctg cgaactttct cgacgtattc
     2581 gccaagcttg tcgatgttca tgcgccaatt gtcgaaattg tcctggcgca tcggcgaatg
     2641 atcggaaaag ccgatttccg tcagccccaa cgccaccgcg cgcgcggcat attcgaccgg
     2701 ttcgccgttt gcgtgatggc agagcggcgt gtgcatgtga tagtcagcag gccaagacat
     2761 gaaaatagtc taacaaaaac ggggcagggg acgagaaaga tttttctgaa tggagaaatg
     2821 gagtggtgga gtattggtaa acaatcagta atccatcatt ccagcatccg aacattgatt
     2881 ccgcgattca cgttctcttg ggacgcaacg aatgccagcc gtggacgcgg tccgcgaggt
     2941 catgcacagt gtctccggca tgagcgggcg cgtgttttgg ttcgccgcga ttttccgatg
     3001 aacgggtgcg cgcgctgccg gcagtcgcag gctgattgga agtccattct gttttcttcg
     3061 gtttgccttg cgtctgtgct tcttcgcgat cttccttggc gccttcgcct tcggatttcc
     3121 attgggcagt ttttgcgggc ttgatggcgc ggccatgctc gctggcgtac acttgggtga
     3181 attcagcgcc atacagaaaa atctgcgcgg aataatagac ccacaccaag atcaagacga
     3241 ttgatcccgc aactccgtac ggcgacgcga ctgagccgta acccagatac aacgccagca
     3301 aaacttttcc caccgtgaac agaaaagccg tcaccaaagc gccgacccac acatcacgcc
     3361 aatgcggccg cgcatcgggc agaaatttga agatcatagt gaacaacacc gtgataactc
     3421 cgcacgaaac caaaaattcc actccgtgcg ccaattgttt gccgcctggg attgagtcaa
     3481 agactgaacc ttgcatggcc gcgatcgcgg tgctcgcaag cagcgaaacg accagcagaa
     3541 tgccgatcgc aaccagaagc acgaaaagca gcgcgcggtt gacgatcatt cgactccagc
     3601 cgacattcga tttttgttca acgccccaca tgctgttgag gtcgctctgc aactcggcaa
     3661 aaacgcccgt ggcactgaga atcaaaacca cgatgccaat ccctgtggca aggggacctt
     3721 tgccatgcac ttgagtttgt gacatgaggc tttggataaa ttggccgctc ttttggccca
     3781 tc
//
LOCUS       DASZMM010000578         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1087710, whole genome shotgun sequence.
ACCESSION   DASZMM010000578 DASZMM010000000
VERSION     DASZMM010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087710"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VG754_13860"
     CDS             <1..1014
                     /locus_tag="VG754_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF20351.1"
                     /translation="EITVKALTLILRSRLRNGDQVFHVAREAASHSNPAVNWPTLRPA
                     SVAADDDSPQVDVQPPALAKGQDSSAAMLPPEIFAPNDGKGPQQLSEKFLAFMPAYVA
                     EVKQHVTALGEMDESAKRKEMAIRLHLRLQCLSRKIDVPTLRPAFELCRSLEGLLKKF
                     VENPKNITETSVRTVSSALDLLKDLCIPGVRPDLVDKPQVRLLVVDDEPLARRALTGA
                     LQMAFTKPLTAETGEEALELAADHEFDIVFMDVCMPGMDGFAACSKIHETSRNNFTPV
                     IFVTTLSDDEFRVRASQCGGADYVVKPFIFMEINLKALTIALRGRLEIDKLAQAPKTP
                     VAA"
     gene            1040..>1611
                     /locus_tag="VG754_13865"
     CDS             1040..>1611
                     /locus_tag="VG754_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF20352.1"
                     /translation="MPAFQYVARDANDGREILNKIMAENEESAIKALLDRNQLVVAIK
                     EEGADARRGTAGGRVGLKDLVGFTRQLATLVDAGMAFVSCLKALGKQQRRKAMKDIIY
                     DLAARVETGDSFSDALTKHPKVFDQLYVCMVKAGEKSGMLAESLSRLALHLEGAARLR
                     RKVKSAVMYPAVVTIVAIAVSIFLMVKVVPV"
BASE COUNT          360 a          455 c          458 g          338 t
ORIGIN      
        1 gagatcacgg tcaaggcgct gactcttatc ttgcgctcgc gccttcgcaa cggcgaccag
       61 gttttccacg tcgcgcgcga ggcggccagc catagcaatc ccgcagtcaa ctggcccacg
      121 cttcgcccgg catcggttgc ggctgacgac gactccccgc aagttgatgt gcaaccaccc
      181 gcccttgcga agggccagga cagttcggca gccatgttgc cgcccgaaat ttttgcgccg
      241 aacgacggca aagggcccca acagctttcc gagaaattcc tcgcgttcat gccggcttat
      301 gtcgccgaag tgaaacagca cgtcactgcg ctcggcgaga tggatgaatc cgcgaagcgg
      361 aaagagatgg caatccggct gcatctgcgt ctccaatgcc tgtcgcgcaa gattgatgtg
      421 ccgacattgc ggccggcttt tgaattgtgc cgttcactgg aaggcttgct gaaaaaattc
      481 gtggaaaatc ccaagaacat cacggagacc tcggtgcgca ctgtgtcgtc cgcattggat
      541 ctgttaaaag atttgtgcat acccggagtg aggcccgacc tcgtcgataa accgcaggta
      601 cgccttctgg ttgtggatga tgaacccctc gcccgccgcg cgttgaccgg cgcgttgcaa
      661 atggcgttca ccaaaccgtt gaccgcggaa acgggagagg aagcgctcga actggccgca
      721 gaccatgaat tcgacatcgt gttcatggat gtttgcatgc cgggcatgga tggcttcgcc
      781 gcctgctcaa agatccatga aacgtcgcgc aataatttta cgccggtcat cttcgtgacg
      841 accctttcgg acgatgaatt ccgggtgcgc gcctcccaat gcggcggcgc tgattacgtg
      901 gtgaagcctt ttattttcat ggaaatcaat ttgaaggcgc tcacgatcgc gctgcgtggc
      961 cgattggaga ttgacaaatt ggcccaagcg ccgaaaacgc ccgtcgcagc ctgacccgaa
     1021 ccgcaaccaa taaactacca tgccagcatt ccaatatgta gccagagacg ccaatgatgg
     1081 ccgggaaatc ctcaacaaga taatggctga gaatgaggaa tccgccatca aggccttgtt
     1141 ggaccgcaat caacttgtgg tcgcgatcaa ggaagaaggc gctgacgccc gccgcggaac
     1201 tgcaggcgga cgggtcggtt tgaaagactt ggttggcttt acacgccagc tcgccacttt
     1261 ggttgacgcc ggcatggcgt tcgtcagttg tttgaaggcc cttgggaaac aacaacgccg
     1321 caaggcgatg aaagacatca tctatgatct tgccgcccgc gttgaaacgg gtgacagctt
     1381 ctcggatgcc ctgacaaaac accccaaggt tttcgaccag ttatatgttt gcatggtgaa
     1441 agccggtgaa aaaagcggca tgttggcgga atcactctcc cgcctcgcgt tgcacctgga
     1501 aggcgcggca agacttcgtc gcaaagtcaa atcggcggtg atgtatcccg ccgtggtcac
     1561 gattgtggcc atcgcggtct ccatcttttt gatggtgaaa gtggtgcctg t
//
LOCUS       DASZMM010000579         6003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_823406, whole genome shotgun sequence.
ACCESSION   DASZMM010000579 DASZMM010000000
VERSION     DASZMM010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823406"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VG754_13870"
     CDS             complement(<1..305)
                     /locus_tag="VG754_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20353.1"
                     /translation="MYFKNLTPEEGTADKLLHDLNVLEENTEQLFFASQGKLTDKSKE
                     KFLRGLERVKATCRTLQEKSHSGQMSTGSGSGAFPYVTMGIVFGLGMLMGALLKRDS"
     gene            454..1614
                     /locus_tag="VG754_13875"
     CDS             454..1614
                     /locus_tag="VG754_13875"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF20354.1"
                     /translation="MLWRPKMREGARGFGLLMIFLFVFLTVTTRAGTLPPIQTVFVIL
                     MENNNWSNIKGNPAAPYINSLLPRASHCEQYTSVYHPSLPNYLWLEAGTNFGISDDND
                     PSSDHQSTTNHLVTLLNNAGITWKAYEEGIPGTNVPLLNTSSGYAVRHDPFAYFDDIT
                     GTNNPNYAYGIAHIRPFTELVGDLASNHVARYNFITPNVCDDMHDTCAPLNNSVLQGD
                     NWLSNNLPIILGSEAYLNGGAVFITWDESEGGTYPIGMIVLSPYARGSGYSNTVSYTH
                     SSTLRTMQEIFGVTPLLRDAANATDLSDLFMPPQTPPAGLEITNFSASANGVKMNVVG
                     VNPGSTNVVQLSTDLAHWNPVSTNVATTSSFAYTNSSPANIGKTFYRVIEMR"
     gene            complement(1634..4978)
                     /locus_tag="VG754_13880"
     CDS             complement(1634..4978)
                     /locus_tag="VG754_13880"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HWF20355.1"
                     /translation="MICFAVRAADSAPTPQQLEFFEKKIRPLLTENCYKCHSHDSEKI
                     KGGLLLDTHDGVLKGGDTGPALVPGNPDKSLIIEAVRYTNKDLQMPPNDRQLENSQIA
                     DLETWVKMGAPDPRTANDAGAHNYAAEAERAKKHWAFQSITHPAVPQPDDSQHWVQTP
                     VDNFILAKLLDQKLTPSPRADKITLLRRATFDLIGLPPTPKEVDDFLADNSPDAFEKV
                     VDRLLASPHYGERWGRYWLDLAHFSDTKGNIGNGQDERYPYAYIYRDYVIRAFNEDLP
                     YNQFLMQQIAADRLPLGDDKRPLAALGFLTLGNRAQAMNDMIDERIDIISKGTMALTV
                     TCARCHDHKFDPIPTKDYYALHGVFNSCIEPKEEPLIETPKDTIAYREFKNEYAKREA
                     AVKTFRAEVNAKLKGEIIGKCATYMLAVHEFHQATNGISRNAFMEKRGLFPQLANGWD
                     DAFKNLSRKHNPVFAPWFAFEQLSDAEFSANAKTLAATFYANEDKKKPINPMIARMFG
                     NTPASLAQVAARYASVFAEVDQHWEEMMFSYEAKSKSATNAIAAPKALSDPGQEQIRQ
                     QLMANQYALLSNERYFGNFLNRDQKTKNKLQGLERDMVELVVTHPGSPARAPVLEDAD
                     KPHDSYVMIKGNPGNRGPVVSRHFLSILSGNTPLTFKDGSGRLDLARDIASKDNPLTA
                     RVIVNRVWLHHFGEGIVRTPDDFGTRGMPPSHPELLDYLASRFMDDGWSIKKLHRVIM
                     LSSAYQQSSDENPRFEQIDPENKWLWRMNRRRLDFEALRDTVLAIGGDLDLSVGGRPV
                     KLDAEPYPLRRTVYGFVDRRNMPNMFQAFDFASPDLVTGKRENTVVPQQALFMMNSPL
                     VVEQARNVVRRVDFKAQANIEARIDLLYKLIYQRTPTDMEMKLAKEYLMSDSATEWQT
                     TPQSAWEYGFSDYVPSLRRLKQFIPMGAFANRAWTPGGKNLDNALRGLSLTPDGGNPG
                     KLYAVVRRWTSPRDGFISIEGTLSHPSKDGDGVQGHIISSRSGELGNWIAYGSQAETK
                     LAHVHVSRGETIDFVTDCRENARGDNFKWAPNIKMEPIPNLPAEAVTEWRAQRDFSGE
                     MRARRLTAWEKFAQVLLETNELTFVN"
     gene            complement(5091..>6003)
                     /locus_tag="VG754_13885"
     CDS             complement(5091..>6003)
                     /locus_tag="VG754_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF20356.1"
                     /translation="SLGSWVTYGLGSENQNLPGFIALSTGGVSPQNWRSAFLPGAYQG
                     TAVNTQNTSIDKLIENIKNKYTSLPEQRKQLDLLHQLNEVHSQNLRKDAQLEARLQAY
                     ELAYQMQMEATDAFDISKEPKEIREMYGDNTPTGRQMLIARRLLEKGVRFVQVWHGGW
                     DHHTNLETAMTKKAQECDKPLAALLTDLKQRDMLKDTLVIWGGEFGRSPTADGNVAGG
                     TPGRTHNNKAFSIWMAGGGVKGGMTYGATDEFGAKAVENRMHIHDLHATILWLLGFDH
                     EKLTYRYNGRDFRLTDVYGSVVKAVMA"
BASE COUNT         1285 a         1730 c         1596 g         1392 t
ORIGIN      
        1 tcggaatcac gcttgagcaa ggcgcccatc aacattccca ggccaaaaac gatgcccatc
       61 gtgacatacg gaaacgcgcc ggagccggag ccggtggaca tctggccgga gtgcgatttt
      121 tcctgaagag tgcggcaagt ggctttcacg cgttccaagc cgcgcaggaa tttctccttc
      181 gatttgtcgg tcagttttcc ttgcgaggcg aagaacaatt gctcggtgtt ttcctcaagg
      241 acattaaggt catgcaacag cttgtccgcc gtgccttctt caggtgtcag atttttgaaa
      301 tacacttcca taacctctaa gtcgctcaac tttcccttat gctaacgccc aaaatccaat
      361 ttttcaagcg ggcattcgcg tcctacgccg ctgtaggata aaaaccgttt tcgacgaatc
      421 acattttagt gcacaataag ccatatggat ccaatgttgt ggcggccaaa aatgcgcgag
      481 ggtgcgcgcg ggttcggcct gctgatgatt ttcttgttcg tttttctgac tgtcaccacc
      541 cgcgccggca cattgccgcc gatacagaca gtgttcgtca ttctgatgga gaataacaat
      601 tggtcgaaca tcaaaggcaa tcctgccgcg ccttacatca attccctgtt gccgagggct
      661 tcacactgcg aacaatacac cagcgtttac catccgagcc tgcccaatta tctctggctg
      721 gaagccggga cgaacttcgg catcagcgat gacaacgatc cctcatcgga ccaccaaagc
      781 accacgaacc atctagtcac cctgctcaac aacgccggga tcacgtggaa ggcgtacgaa
      841 gaaggcatcc cgggaacgaa tgttccgctc ctgaatacca gcagcggata tgccgtccgg
      901 cacgatccat tcgcctattt tgatgatatc acaggcacca ataatccgaa ctatgcttac
      961 ggcatcgcgc atatccgtcc tttcacggaa ctggttggcg acctcgcaag caaccacgtg
     1021 gcccgctaca atttcatcac cccgaatgtc tgcgacgaca tgcatgacac ctgcgcgcca
     1081 ctcaacaatt cagtcctgca aggcgacaac tggctctcca acaatctgcc aatcatcctt
     1141 ggctcggaag cgtatctgaa tggcggcgct gtgttcatca cctgggatga atctgaaggc
     1201 gggacgtatc ccattggcat gattgtcctg tctccttacg cccgcggcag tggttactct
     1261 aatacggtct cctacactca cagttccaca ttgcgcacga tgcaggaaat ttttggggtt
     1321 acgccgctgc tccgcgatgc cgcgaatgcc actgatttga gcgacctgtt tatgccgccg
     1381 caaacgccgc ccgccggttt ggagatcacc aacttttccg cctcagccaa tggagtgaag
     1441 atgaatgtcg tcggtgtgaa tcccggcagc acaaatgtgg tccaactctc aacagacttg
     1501 gcgcactgga accccgtgag cactaatgtg gcgacaacaa gctctttcgc gtacaccaat
     1561 tcttcgccgg caaatatcgg aaaaactttc tatcgcgtga tcgagatgag atagccgcac
     1621 gcttcgaaaa ccgctaattc acgaatgtca attcgttggt ctcaagcaac acctgcgcaa
     1681 atttctccca agcggtcaaa cggcgcgccc gcatctcgcc gctgaaatcg cgctgggcgc
     1741 gccattccgt caccgcctcc gcaggcaggt tcggaatcgg ctccatcttg atgttcggcg
     1801 cccatttgaa attgtcgccg cgcgcgtttt cccggcaatc cgtgacgaaa tcaatcgtct
     1861 cgccgcgaga gacatgcaca tgcgcgagct tcgtttccgc ctggctgccg tatgcaatcc
     1921 aatttcccaa ttcacccgaa cggctcgaaa tgatgtgacc ctgcacacca tcaccgtcct
     1981 tcgatggatg tgaaagtgtt ccctcgatgc tgatgaaacc gtcgcgcggt gaagtccatc
     2041 ggcgcaccac cgcataaagt ttgccgggat tgccaccgtc gggagtgagg ctcagtccgc
     2101 gcaacgcgtt gtcgagattc ttaccgccgg gcgtccatgc acggttggca aatgcgccca
     2161 tcgggatgaa ttgcttgagc cgtctcaacg atggaacata atcgctgaag ccatattccc
     2221 aggcggattg cggcgtggtc tgccattcgg tggcggaatc cgacatgaga tattccttcg
     2281 cgagcttcat ctccatgtcc gtcggagtgc gctgataaat taatttataa agcagatcaa
     2341 tgcgcgcctc gatgttggcc tgcgccttga aatcgacgcg tcgcaccacg ttgcgcgcct
     2401 gctcgaccac aagcgggctg ttcatcatga acaatgcctg ctgcggcacg acggtgtttt
     2461 cacgttttcc ggtgacgaga tcggggctgg cgaaatcaaa ggcctgaaac atgttcggca
     2521 tgttgcggcg atccacaaaa ccgtacaccg tacgtcgcaa tggatacggt tccgcgtcaa
     2581 gtttcaccgg gcgtccgccg acgctcaagt ccaggtcgcc gccaatggcg agaacggtgt
     2641 cgcgcaacgc ttcgaaatca agccggcggc ggttcattcg ccagagccat ttgttctccg
     2701 gatcaatttg ttcgaagcgc gggttctcat cgctgctctg ctgataagcg ctggaaagca
     2761 tgattacgcg gtgcagtttc ttgatggacc agccatcgtc catgaaccgc gacgcgagat
     2821 aatcgagcaa ttccggatgc gacggcggca ttccgcgcgt cccaaaatcg tccggcgtcc
     2881 gcacgatgcc ttcgccgaaa tgatgcagcc aaacgcggtt gacgatgaca cgggccgtca
     2941 gcgggttgtc cttgcttgcg atgtcgcgcg ccaggtccag ccgaccgctt ccgtccttga
     3001 atgtcaacgg cgtgttgccc gaaaggatgc tcaggaaatg acgtgaaaca accggcccgc
     3061 gattgccggg attgcccttg atcatcacgt aggaatcatg cggcttgtct gcgtcctcca
     3121 acaccggcgc gcgcgctggc gaaccgggat gcgtcaccac cagctccacc atgtcgcgct
     3181 caagcccttg aagtttgttt ttcgttttct gatcgcgatt gaggaaattt ccaaagtacc
     3241 gttcgtttga aagcaacgca tattgattgg ccatcaattg ctgccggatc tgttcctgac
     3301 cggggtcaga aagagccttg ggcgcggcaa tcgcattggt ggcggatttc gatttggctt
     3361 cgtaagagaa catcatttcc tcccaatgct ggtccacctc ggcaaagaca gaagcataac
     3421 gcgccgcgac ttgcgccagc gaagccggcg tgttcccgaa catccgggca atcatcgggt
     3481 tgatgggctt cttcttgtcc tcgttcgcat aaaacgtcgc ggcgagtgtt ttggcatttg
     3541 ctgaaaattc cgcatcgctc aattgttcaa acgcaaacca aggagcgaaa acgggattgt
     3601 gtttgcgcga caaattttta aatgcgtcgt cccaaccgtt ggcaagctgc ggaaacagcc
     3661 cgcgtttttc catgaacgca ttgcgcgaaa tgccattcgt cgcctgatga aattcatgca
     3721 ccgccagcat gtaagtcgcg catttgccga tgatctcgcc cttcaatttc gcattgacct
     3781 cggcgcgaaa tgtcttcacc gccgcttcgc gcttcgcgta ttcattcttg aattcgcgat
     3841 aggcaatcgt gtccttcggc gtctcgatca gcggttcttc cttcggttcg atgcaactgt
     3901 tgaacacccc gtgcagcgcg taataatcct tcgtgggaat cgggtcgaac ttgtggtcgt
     3961 gacaacgcgc gcaagtcacc gtcaatgcca tcgtgccctt ggaaatgatg tcgatgcgct
     4021 catcaatcat gtcgttcatg gcctgcgcgc gattgccgag cgtgagaaaa ccgagcgcgg
     4081 caaggggccg cttgtcgtcg cccagcggca ggcggtcggc ggcgatttgc tgcatcaaaa
     4141 attgattgta aggcaggtcc tcattgaacg cgcggatgac gtaatcgcgg taaatataag
     4201 cgtacggata gcgttcatcc tggccgttgc cgatgttgcc tttcgtgtct gaaaaatgcg
     4261 ccaagtcgag ccagtaacgc ccccaacgct cgccgtaatg cggtgacgcc agcaatcggt
     4321 ccacgacttt ttcaaaagcg tcgggcgaat tgtccgccag gaaatcatca acttccttgg
     4381 gcgtcggcgg caagccgatc aaatcgaatg tcgcgcggcg caataaagtg atcttgtcag
     4441 cgcgcggaga aggcgtgagt ttttggtcaa gcaatttggc gaggatgaaa ttgtccacgg
     4501 gcgtttgcac ccaatgttgc gaatcatcgg gctgcgggac ggccggatgc gtaatggatt
     4561 ggaacgccca atgttttttt gcgcgctcgg cttcagcagc gtaattatgc gcgccagcgt
     4621 cgttcgctgt gcgcggatca ggcgcgccca ttttcaccca tgtttccaga tcggcgatct
     4681 ggctgttttc aagctgccgg tcgttgggcg gcatctgcaa atccttgttc gtgtaacgga
     4741 ccgcctcaat gatcaggctt ttgtcggggt tgccgggaac caatgccggg ccggtgtcac
     4801 cgcccttgag cacgccgtcg tgcgtgtcga gaagaagccc gcccttgatt ttctcgctgt
     4861 catggctgtg gcacttgtag caattctcgg tcagcaatgg ccggattttt ttctcgaaaa
     4921 attcgagttg ttggggagtg ggagcggagt cggcagcgcg aacagcaaaa caaatcaaaa
     4981 gccaaatgaa aagagaaaca aacgctgaac gctgaacatc gaacgccgaa catcgaattg
     5041 cagaaactcg gcgttcgacg ttcgatgttg gatgttcgat gtttcccatt tcacgccatg
     5101 acagctttaa ccacacttcc atacacatcc gtgagacgga aatcgcggcc attgtagcga
     5161 taggtgagct tctcgtgatc gaacccgagg agccagagaa tcgtcgcgtg caaatcgtgg
     5221 atatgcatgc ggttttcaac ggccttcgcg ccgaattcat cggtcgcgcc gtaagtcatg
     5281 ccgcctttga cgccgccgcc ggccatccaa atgctaaatg ccttgttgtt gtgcgtgcgg
     5341 cccggtgtgc cgcctgcgac gtttccgtcc gcggtcggcg aacggccaaa ttcaccgccc
     5401 caaatcacaa gcgtgtcctt gagcatgtcg cgctgcttga ggtcggtcag caacgccgcc
     5461 agcggcttgt cgcattcctg ggccttcttg gtcatcgcgg tttccaaatt ggtgtgatgg
     5521 tcccagccgc cgtgccagac ttgcacgaag cgaacgccct tttccagcaa tcgccgcgca
     5581 atcaacatct gccggcccgt cggcgtattg tctccataca tctcgcggat ttccttcggc
     5641 tccttgctga tgtcaaaggc gtcggtcgct tccatctgca tctggtaagc cagttcataa
     5701 gcctgcaaac gcgcctccaa ctgcgcgtcc ttccgcaagt tctggctgtg gacttcgttg
     5761 agctgatgca gcagatccag ttgtttgcgc tgttcaggaa gcgaggtgta tttgtttttg
     5821 atgttctcga tgagcttgtc gatcgaggtg ttttgcgtgt tgaccgcggt accctggtaa
     5881 gcgccgggca aaaaagccga acgccaattc tgcggcgaaa ctccgccggt ggacaacgcg
     5941 atgaaccccg gcaaattctg gttctcgctt cccaaaccgt aagtcaccca cgaaccgagg
     6001 ctc
//
LOCUS       DASZMM010000580         9101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1088375, whole genome shotgun sequence.
ACCESSION   DASZMM010000580 DASZMM010000000
VERSION     DASZMM010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088375"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VG754_13890"
     CDS             complement(<1..466)
                     /locus_tag="VG754_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWF20357.1"
                     /translation="MLRRNRQIKMQIFQVLDAILFGLSFWLAWFLRSNPDIIDLFNLK
                     PVHSFDAYFWLYLLLIIGGPLVLEAQGFYDRPLLSPRRQIIWPLFKGCLVTTVALIMV
                     LFFFRVELARGVAVLLGGISFVLIYIKEELVRSGLRSKFALAQLQRKYILVGT"
     gene            complement(566..1318)
                     /locus_tag="VG754_13895"
     CDS             complement(566..1318)
                     /locus_tag="VG754_13895"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HWF20358.1"
                     /translation="MNKSNRTILIVALLMIGGAALGINAMKAHQRLSPPGIITKPIPG
                     SQRLDVYLPENVLDYQSIGLPTDKNMLIYLPQDTSFRTRQYRRNEDVVTVNVVLMGAD
                     RTSIHKPQFCLRGQGWDIDAENSASDTVRVESPHPYDLPVMKLVNSRDFNDKQGNLIK
                     VRGIYVYWFVADHDLTADHWTRMRKMATHLLRTGELERWAYVSCFSVCLPGNEDATYA
                     RMKKFIAAAVPQFQLTVNPSVASLETPQTASR"
     gene            complement(1315..2349)
                     /locus_tag="VG754_13900"
     CDS             complement(1315..2349)
                     /locus_tag="VG754_13900"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HWF20359.1"
                     /translation="MNSKQNSISLRPSLLDELMGGLRAFPAEFSACWQQLPNKVLFFT
                     LLEAWLLLFQFLGNGTFGYVNTKSIMYWMYNAYNNEQSDGQDAHGNLIPFVVLGLMWW
                     KRKELLGLQIRSWWPGLALLGGALALHVLGYRVQQPRISIVAFFAGIYALMGLAWGPS
                     WMRASFFPFFLFVFCIPIASIGEPVTFPLRHLVAGIVAFICNKILFLDVVREGTQLFN
                     ASHTYAYEVAAACSGLRSMVAIFALSTIYGFLTFEHNWKRVLMMLVSFPLAVIGNVTR
                     MLCIILAAEISGQHAGDFVHENWFFSLVPYVPAFIGVYFMARWLGETGKAPSAATPGN
                     APSTATPKSA"
     gene            complement(2395..3951)
                     /locus_tag="VG754_13905"
     CDS             complement(2395..3951)
                     /locus_tag="VG754_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20360.1"
                     /translation="MNASSAQADAGETKTGPQWRWAFLGVLMCFAAITHQSLWNDEAL
                     TAVKAMQPSLHDWWHEMLGDKASDLQMPLYMIYMWGFAKLSGTSEWALRCANIPWFVT
                     GLWAFMRGLTGPKKMSMAFIVLLSPFAWYYIGEARPYAMQLGASLMIFAALYRLGKDK
                     VKVMKGERGWVLLFLFGVVSLSGSSLLGMIWAGAGCLAAGFVLGLDALTKLVRTNLVL
                     CLISFFLLAGLAGYYLWTLQIGARASDVGRTDARNLVFIIYELLGFGGLGPGRLEIRE
                     EGWRAFLPFAKSLFIYGMLILDLLVLGGWRICREQTRTKLCAIVLIIVLPMVTILGAG
                     YVKEFRVLGRHFTPLLPVILFILWVGLQARWSRPHWASRPLAIGFVILTLASCLSVRF
                     AARHERDDYRDAAGFAKAALETGREVWWNAGREGAVYYELPLAETPNTSNAALWLMNP
                     TVKMLQDSPSPEIVITSKPDVYDGNRIIAEYLAQGNYTKVGALPAFIIWQRKTAFNAS
                     PQQSVHGEFD"
     gene            complement(3967..4935)
                     /locus_tag="VG754_13910"
     CDS             complement(3967..4935)
                     /locus_tag="VG754_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF20361.1"
                     /translation="MSKIVVCGGGGFIGGHLVMDLLRQGHTDIRSVDIKPFNEWYQFS
                     PKVENLQLDLQEKEACEKALAGAHTVYNLAADMGGMGFIENNRALCMLSVLINTHLCM
                     AAKKHGVQRFFYASSACVYAADKQTNTNVTALKESDAYPAMPEDGYGWEKLFSERMCR
                     HFREDFGLVTRVARYHNVYGPFGTYDGGREKAPAAVCRKVITAKLSGKHEIEIWGDGH
                     QTRSFMYIDDCLKGTQAILGSDILEPINLGSSELVSINQLVDIVEGIGEVKLKRNYNL
                     GAPKGVKGRNSDNTLIRKYLNWEPNVKLKDGLEKTYRWIHDQMTKT"
     gene            complement(4984..5763)
                     /locus_tag="VG754_13915"
     CDS             complement(4984..5763)
                     /locus_tag="VG754_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWF20362.1"
                     /translation="MSDATQFQKVNVLGVGLSAINLKLALEAVGRALAQKTKGYVCVT
                     GVHGVMEAQQDSNFRAILNRSFLNTPDGMPMVWIGRLNGFREMDRVYGPDLMLLVCEY
                     ARDHGYTHFLYGGAEGVAQQLERSLETKFPGIKIAGTYTPPFRPLNETEESELIKLIS
                     ERKPDIIWVGLSTPKQEKFMAQYQDKFPATLMFGVGAAFDFHAGRVRQAPRWMQRSGL
                     EWFFRLCCEPRRLWRRYLRNNPLFAFRFFCQWTGIKKYALE"
     gene            complement(6365..7339)
                     /locus_tag="VG754_13920"
     CDS             complement(6365..7339)
                     /locus_tag="VG754_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20363.1"
                     /translation="MFKNIFLAGLLAVSVAKAEGKDTIAYHAAEVPLDGLYFYVDLDQ
                     DGSFDYFLVGLTVISTYSDGPATLTPELTTYSFNGNDYLTDTNGNIQLEPAGYTFGPD
                     TLSGQTWGQIGSSLPLLSSGSGDYWSGFGGEPPECFIGVRFLTGGEQHYGWIRLLMPT
                     RQPPQAGAQATVFPVVADWAYETRPDTAIRAGETGQDDEPELFTVDFENPDGTPAESD
                     IRRSSGSFILQGDTLHYEMVVVGTHTNLDVLDPADSMPVFSMVEAPTVLPGFPTYVIG
                     GIHQLSHPQIAQIRHGSYYVSVDQGVVLGRIVPAKESPLDKIANSSRP"
     gene            complement(7861..>9101)
                     /locus_tag="VG754_13925"
     CDS             complement(7861..>9101)
                     /locus_tag="VG754_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HWF20364.1"
                     /translation="LCDAVLGKDNFRSEIVWKRTSSHNSAKRFAPVHDVILLYSKAGD
                     YIWNQVFQQYSEEYLESFYRFTDAKGRFRIGDLTGAGRRTGESGKPWRKVDPTEVGRH
                     WAVPTKVLVELFGDKSETWSVPDKLDALDKAGLIYWPPKGKVPGFKRYYNPDAGVPAA
                     DIITDIDPIAAQSAERLGYPTQKPVALLERFIKASSNPGDLVLDPFCGCGTTIDAAEK
                     LGRKWIGIDITQLAASLIKNRLRDTYGEKIEIITIGEPTTPNEAAVLAEQDKYQFQWW
                     ALGLVGARPVEQKKGADHGVDGKILFRDDPKSGKPEQIVFQVKGGKTGVKDVRDLRGV
                     LDREKAAIGVLISLQPPTGPMETEAADVGFYEHKTNKQKYPRLQLRTVKELMEGRGIE
                     RPTTAASVDDTFKKAPVSKK"
BASE COUNT         2148 a         2630 c         2316 g         2007 t
ORIGIN      
        1 gcgtgcccac gaggatgtat ttgcgctgca attgggcgag cgcgaatttg cttcgcaagc
       61 ccgaccgcac cagttcctcc ttgatgtaaa tcaacacgaa gctgatgccc cccagcaaca
      121 ccgccacgcc acgggcgagt tcaacgcgaa agaagaacag caccatgatc aacgccaccg
      181 tcgtaaccaa acaacccttg aacaatggcc agatgatctg ccggcgcggg gaaagcagcg
      241 gacggtcata aaaaccttgc gcttcgagca ccagcggccc gccgatgatc aacaggagat
      301 aaagccagaa ataggcgtcg aaggaatgga ccggcttgag gttgaacagg tcgatgatgt
      361 ccggattcga gcgaaggaac cacgccagcc agaagctgag gccgaacaga atggcatcca
      421 gcacctggaa tatctgcatt ttgatctggc gattgcggcg cagcatggtt tcttcaaatc
      481 acacgattgt cggacaaacg aacattgtta tcaaaacgga gctttcagtt caatgacttc
      541 ggcagtcggc gcgccaacaa agcggtcagc gcgaggccgt ctgcggtgtt tccaacgacg
      601 caacggacgg attgactgtc agttgaaact gcggcaccgc ggcagcgata aatttcttca
      661 tgcgcgcgta agtcgcatct tcattcccgg gcagacagac cgagaagcag cttacgtacg
      721 cccagcgctc cagttcgccc gtccgcaaca aatgggtcgc catcttccgc atccgcgtcc
      781 agtggtcggc agtcaaatcg tggtcagcga caaaccaata aacataaatc ccgcgcacct
      841 tgatgaggtt cccctgcttg tcgttgaagt cccgggaatt cacgagtttc atcacgggca
      901 aatcataagg atgcgggctt tctacgcgca ccgtgtcgga agcggaattc tcggcatcaa
      961 tgtcccatcc ctggccgcgc aggcagaatt gcggcttgtg aatgcttgtc cggtccgctc
     1021 ccatcagcac cacattcaca gtgaccacgt cttcgttgcg ccgatattgc cgcgtccgaa
     1081 agctggtgtc ctgcggcagg tagatgagca tgttcttgtc tgtcgggagg ccaatggatt
     1141 ggtaatcgag cacgttctcc ggaagataaa cgtccagtcg ctggctgccg ggtatcggtt
     1201 ttgtgatgat gcccggcggg gaaagcctct ggtgggcctt catcgcgttg atccccaaag
     1261 ccgcgccgcc aatcatcagc aacgccacga tcaagatggt ccggttgctt ttgttcatgc
     1321 ggatttggga gttgcggttg acggtgcatt gccaggggtt gcggcggacg gcgccttgcc
     1381 agtctcgcca agccaccgcg ccatgaaata cacaccgatg aacgcaggca catacgggac
     1441 aaggctgaaa aaccagtttt catgcacgaa atcgcccgcg tgctggccgg aaatctccgc
     1501 ggcaagaatg atgcacaaca tccgggtgac attgccgatg accgccagcg gaaatgacac
     1561 gagcatcatc aaaacccgtt tccaattatg ttcaaatgtc agaaatccat agatcgtcga
     1621 gagagcaaag atggcgacca tgcttcgcag cccgctgcaa gccgccgcca cttcataagc
     1681 ataagtatgg cttgcattga acaactgcgt gccttcccgc accacatcga gaaaaagaat
     1741 cttgttgcag ataaacgcga cgattcccgc caccagatgg cgcaacggaa aagttaccgg
     1801 ttcgccaatg gatgcgatgg gaatgcaaaa gacaaaaagg aaaaatggaa agaaacttgc
     1861 ccgcatccac gaaggccccc atgccagacc catcagcgca tagatgcccg caaaaaaagc
     1921 gacgatcgaa atgcggggtt gttggacgcg gtatcccagc acatgcaacg cgagtgcccc
     1981 cccaagcagc gccaatcccg gccaccacga acggatttgc agccccaaca attccttgcg
     2041 tttccaccac atcaaaccaa gcaccacaaa aggaatcagg ttcccgtgcg catcctgtcc
     2101 gtcgctctgt tcattgttgt acgcattata catccagtac atgatggatt tcgtgttgac
     2161 gtacccgaaa gtgccgttcc cgagaaattg gaacagcaaa agccacgctt ccagcaaggt
     2221 gaaaaaaagc actttgttcg gaagttgctg ccagcaagca gaaaactccg ccggaaaagc
     2281 gcgcaaaccg cccatcaact cgtcgagcaa gcttggcctg aggcttatgc tgttttgttt
     2341 gctattcatt cgcgggcacc cggtgctcat tagaccatga acgcccggat ttgctcaatc
     2401 aaattcgcca tggacgctct gctgaggcga agcgttgaag gccgttttcc gctgccagat
     2461 gatgaatgcc ggcaaggcgc cgaccttggt gtaattgcct tgagcgagat actcggcaat
     2521 aatgcggttc ccgtcgtaaa catccggctt ggacgtgatg acgatttcag gcgaagggga
     2581 atcttgcaac attttaacgg tgggattcat cagccaaagc gccgcattgg aagtgttcgg
     2641 cgtctccgcc aacggaagtt cgtaataaac cgccccttcc cttcccgcgt tccaccaaac
     2701 ctctctgccc gtttccaatg ctgccttcgc gaatcccgcc gcgtcccgat aatcgtcccg
     2761 ctcatgcctc gccgcgaagc gaaccgacaa gcacgatgcc agtgtcaata tcacgaaccc
     2821 gatcgcgagc ggcctggacg cccaatgcgg ccgcgaccag cgtgcctgca atcccaccca
     2881 caaaataaac agaattactg gcaacaatgg tgtgaaatgc ctgcccaata cccggaattc
     2941 cttcacataa cctgctccca aaatcgtcac catcggcaaa acaatgatga gaacgattgc
     3001 gcacaatttc gtcctcgttt gttcccggca gatccgccat ccgcccaaaa ccagcaaatc
     3061 caggatcaac atgccgtaaa tgaaaagcga tttcgcgaat ggcaaaaacg ctctccaacc
     3121 ttcttcgcga atttcaagcc gtcccggccc caaaccgccg aacccgagca attcgtaaat
     3181 gatgaaaacc agattgcgcg catccgtccg accgacatcg gaagctcgcg cgccgatttg
     3241 cagcgtccaa aggtaataac ctgcaagccc tgccaataaa aaaaatgaaa tcaagcaaag
     3301 aaccaggttc gttcgcacaa gcttcgtcaa agcatccaga cccaaaacga agcccgcagc
     3361 cagacaacct gctcccgccc aaatcattcc cagcaaactg ctgccggaaa gtgaaacaac
     3421 gccgaagaga aaaagaagca cccatccacg ttcgcctttc attactttta ccttgtcttt
     3481 gccaaggcga tacaatgccg caaaaatcat caggctcgcg ccaagttgca tcgcatacgg
     3541 acgcgcttct ccgatgtaat accatgcaaa gggagaaaga aggacaatga aagccatgga
     3601 cattttttta ggcccggtca aaccccgcat gaacgcccag aggccggtga caaaccacgg
     3661 gatgtttgcg caacgcaagg cccattcact cgtgccagat aatttggcaa aaccccacat
     3721 gtaaatcatg tagagcggca tctgcaaatc ggacgccttg tcccccaaca tctcatgcca
     3781 ccaatcatgc aagctcggct gcatcgcctt gacggctgtc aacgcctcat cattccacaa
     3841 gctttgatgt gtgatggccg cgaagcacat caagacgccg aggaacgccc atctccactg
     3901 cgggcccgtc ttcgtttcgc cggcgtcagc ttgagcggac gaagcattca ttcggcggcg
     3961 agttgcttac gtcttggtca tctggtcgtg gatccagcgg taagtctttt ccaatccgtc
     4021 cttcagcttc acgttcggct cccagttcag atatttccgg atgagcgtgt tgtcgctgtt
     4081 gcggcctttc actcccttgg gcgcgcccag gttgtaattg cgtttcagtt tcacttctcc
     4141 aatcccttcc acgatgtcca ccaattggtt gatcgaaacc agttcgctgc tgcccaaatt
     4201 gatcggctcc aaaatgtcgc tccccaatat cgcctgcgtc cctttcaagc aatcgtctat
     4261 atacatgaag ctgcgggtct ggtgtccatc accccaaatc tctatctcat gcttcccgct
     4321 caatttggcc gtgatcacct tccggcacac tgccgcgggc gctttctcgc gtccgccgtc
     4381 ataggtgcca aacggcccat atacattgtg ataacgcgcc acgcgagtca ccaatccgaa
     4441 atcttcccgg aaatgccggc acatccgttc gctgaacagt ttttcccaac cgtaaccatc
     4501 ctccggcatc gcgggataag cgtcgctttc cttcaatgca gtcacgttgg tgttggtctg
     4561 cttgtccgcc gcatagacgc aggcggagga ggcgtagaaa aatctctgca cgccatgttt
     4621 tttggccgcc atgcaaaggt gggtgttgat gagcaccgag agcatgcaaa gcgcgcggtt
     4681 gttctcgatg aagcccattc cgcccatgtc ggcggccaga ttgtaaaccg tgtgcgcgcc
     4741 ggcaagggcc ttttcgcagg cttccttttc ttgaaggtcg agctgaaggt tctcgacctt
     4801 gggcgagaat tgataccatt cattgaacgg tttgatgtcc acggaacgga tgtccgtgtg
     4861 gccctgccgc aggaggtcca tcaccaagtg gccgccgatg aatccgccgc cgccacatac
     4921 aacgattttg ctcataatgt tttcgataga aagttgagcg tgaagcctaa ttggggggca
     4981 aatttattca agggcatatt tcttgattcc ggtccattgg cagaagaagc gaaacgcaaa
     5041 caagggattg ttccgaagat accggcgcca aagccggcgc ggttcgcagc aaagccggaa
     5101 aaaccattcc aacccgctcc gctgcatcca acgcggcgcc tggcggacac ggcccgcgtg
     5161 aaaatcaaat gccgccccaa caccaaacat cagcgtggca ggaaacttgt cctggtattg
     5221 cgccatgaat ttttcctgct tgggcgtgct caggccgacc cagatgatgt ccggttttct
     5281 ttccgagatg agcttgatca actcactctc ctccgtttcg ttcaacggcc gaaaaggggg
     5341 cgtgtaagtg ccggcaattt tgatgcccgg aaatttcgtt tccaggctgc gttccaattg
     5401 ttgcgccacg ccctccgcgc caccgtagag aaaatgcgtg tagccatggt cgcgcgcgta
     5461 ttcgcaaacc agcagcatca ggtcggggcc gtaaacgcgg tccatttccc gaaaaccgtt
     5521 aagacggcca atccagacca tcggcatgcc atcgggcgtg ttcaggaacg agcggttcag
     5581 aatcgcgcgg aagttcgaat cctgctgcgc ttccatgacg ccgtgcacac cggtgacgca
     5641 cacatagcct ttggttttct gcgccaaagc gcgaccgaca gcctccagcg caagcttgag
     5701 attgatcgca ctcaggccga cacccaggac attgactttt tggaactgag ttgcatccga
     5761 cataaaaaat tggtgcgggg caacatttgt acgcgccaca gcgaacttcg agcgtaacct
     5821 ccaacatcgc ccattttcaa ggcacaaaag gggctcgcca atatcagcct gcgttgacac
     5881 tgcgccgcaa cttcggaaac ctgtggcgga ctgacgccgg cccttgtagc gcagattttt
     5941 aatctgctgt ttcgccgact tgcagtcggc aggacggtcg atgactcggg aagttttgga
     6001 ttatctaaaa tcgttatgcc ctcgcgcggc ttgcggagtc aaactccgcg atacagcaga
     6061 gtgcaactct gcgctactct ttggctgcgg ggcaacccat tccgggggca gtgtgcggat
     6121 gcgcgccatg tcaggatgca tccgcggagc gcggggtcgg cgacccgcag caatgtggga
     6181 agctcgggcg catatgaacc agtcgggcgt gtcttgattg ccatgcattg ctgcggctca
     6241 tcgagccgcg ccccgcttcg caggtgaacg tgtggcgata gccaaccacc ccatccagac
     6301 atgccggcaa attggaattg ggggcatttc gcagatgcgc ctttcgtgcg gaagcattcc
     6361 cctttcaggg cctggaactg ttcgcgattt tatccagggg tgactctttt gcggggacaa
     6421 tcctgccgag gacgacgccc tggtccacgc ttacgtaata gctgccgtgc cgaatttggg
     6481 cgatttgcgg atgggacaat tggtggattc ccccgatgac atacgtggga aatcccggca
     6541 aaacagtagg tgcctccacc atgctgaaca ccggcattga atcagcagga tcgaggacat
     6601 ccaggttcgt atgagtgccc accaccacca tctcataatg gagcgtgtcg ccctgcaaaa
     6661 tgaaggagcc ggagctgcgc cgtatatcag attcagcagg cgtgccgtcg ggattctcaa
     6721 aatccaccgt gaacaattcc ggctcatcat cctgcccggt ttcgcctgcg cgaatggctg
     6781 tatccggcct tgtttcgtat gcccaatccg caaccaccgg gaagacagtt gcctgggcgc
     6841 ctgcttgagg cggttgacga gtcggcatca acaggcggat ccatccgtaa tgctgctctc
     6901 cgcctgttag aaaccggact ccaatgaaac actccggcgg ttctccgcca aatccactcc
     6961 agtaatcccc gcttccggaa cttagcaacg gcaacgaaga accaatctgt ccccaggttt
     7021 gtccagacag agtgtcaggg ccaaaggtgt atccggctgg ttccagttgg atatttccat
     7081 tcgtgtccgt caaataatca ttgccgttga aagaatacgt ggttagttca ggcgtcaacg
     7141 tcgcgggacc atcggaatac gtgctgatca ccgttaatcc gaccaggaaa taatcaaaac
     7201 tgccatcctg gtccaaatcc acataaaaat acaacccgtc cagcggcact tcggcggcgt
     7261 ggtaggcgat ggtgtccttg ccttcagcct ttgcgactga gacagccagc aatcccgcaa
     7321 gaaagatatt tttgaacacg ttgctctccg agtcgtttga tgttataaac tgccaatcct
     7381 cctgtcaatg gcgagaagca aaggtagtgc cctaatttga tgaaaactaa ccccacagat
     7441 tcggagacga ccgcagatgc tgaatccaat gatgctcaat atctgtccac gaatctgcgg
     7501 ggcaaaacgt ttagatcgga acgggccttc agccctaaat actggtttgt ccgcgtgttc
     7561 ctggggcgct gtcccagtct ggtataattc gcgctttcgg tgatcgccgg gcgaaatttc
     7621 ttgctagtaa aaattaaccg aaaaggctca tcgattgaat aggggaagag tcggagccga
     7681 cttacgtcgg ctgctgcttt aaattcgtgc agcagcggac gtgagtccgc tccattcccc
     7741 ggtgggacgg cgctgcgccg tccggcaaaa acggacatcg cagcgcgatg tccctacctt
     7801 tttggccgcc aatgagtata gctcgcactg tcaaatcccc aaatttgtct gctctctttc
     7861 ctacttcttc gataccggcg cttttttgaa ggtgtcgtcc acgctggcgg cggtggtggg
     7921 gcgttcgatt ccacggcctt ccatcaattc tttcacggtg cgcagttgca ggcgcggata
     7981 tttttgtttg ttggtcttgt gctcgtaaaa accgacgtcc gcagcttcgg tttccatcgg
     8041 gccggtgggc ggttgcaggg aaatcaaaac accgatggcc gctttctcgc ggtcaagcac
     8101 gccgcgcaaa tcgcgaacgt cctttactcc cgtcttgccg ccttttacct gaaaaacaat
     8161 ctgttccggc ttgccggatt ttgggtcatc acgaaaaagg atttttccgt caacgccatg
     8221 gtccgcgcct ttcttctgct cgaccggacg cgcgccgacg agtcccagcg cccaccattg
     8281 gaattgatat ttgtcctgct cggcaaggac tgcggcttca ttcggcgtgg ttggttctcc
     8341 gatggtgatg atttcgattt tttcgccgta cgtgtcacgc aagcggtttt tgatgagcga
     8401 agcggccaat tgcgtgatgt ctataccgat ccatttgcgg ccaagtttct cggcggcatc
     8461 aatcgtcgtg ccgcaaccgc aaaacggatc cagaaccaaa tcaccgggat tcgaactggc
     8521 tttgatgaag cgttccaata aggcaacagg tttttgcgtt gggtagccga ggcgttcggc
     8581 agattgtgca gcaattggat cgatatcggt gattatgtct gccgcaggca caccagcatc
     8641 tggattataa tatcgcttga atccaggaac ctttcccttg ggtggccagt aaatcagccc
     8701 tgctttatca agagcgtcca gtttatctgg taccgaccaa gtctcgctct tatcaccgaa
     8761 caattcgact aataccttgg tcggaactgc ccaatgacgt ccgacttctg ttggatccac
     8821 cttccgccaa ggttttcccg attcacccgt cctccttccc gcgccggtta aatcaccaat
     8881 cctaaatcga ccttttgcat ccgtgaaacg ataaaagctt tcaagatatt cctctgagta
     8941 ctgctgaaat acttggttcc aaatgtaatc gccagctttt gagtaaagca aaatcacgtc
     9001 atggactgga gcaaatcttt tggcactgtt atgggaactg gttcttttcc aaacaatttc
     9061 actgcggaaa ttgtctttgc ctaggactgc atcgcaaagc a
//
LOCUS       DASZMM010000581        13440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4930789, whole genome shotgun sequence.
ACCESSION   DASZMM010000581 DASZMM010000000
VERSION     DASZMM010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4930789"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..480
                     /locus_tag="VG754_13930"
     CDS             160..480
                     /locus_tag="VG754_13930"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWF20365.1"
                     /translation="MQQWLAANQIKTIYIEPGSPWQNGFVESFHGRFRDECLNREQLW
                     TLTEAPVVVGDYRQEYNQLRPHSRLGYETPASFAARTCPSPSPIGLRPPSDKDGQTTQ
                     NTTT"
     gene            complement(553..927)
                     /locus_tag="VG754_13935"
     CDS             complement(553..927)
                     /locus_tag="VG754_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF20366.1"
                     /translation="MPHETTRKRAAISIPESCFSLIPELKTLGRNIRDRRKRSGLSQE
                     NLAEKAGLHRTYVCDIERGVRNVSFHSLFRLAEGLSTSISDLMENIGSVSHRDARATP
                     SHTVSFPHQAVPRVDTSERAAL"
     gene            complement(1052..4423)
                     /locus_tag="VG754_13940"
     CDS             complement(1052..4423)
                     /locus_tag="VG754_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWF20367.1"
                     /translation="MGRPISTDSGLSRSGVIRGRALSPGPPVIPDHKMLRCIGVGSYG
                     EVWLAQHCLGMFRAVKVVSRSSFETNRPFERELLGIRQFEPISRSHDGFVDILHVGID
                     QAANCFYYVMELGDDVELGKAIRPDSYSPRTLRREISRRSRLSVRECADLGLAMCGAL
                     ERIHDEGLVHRDVKPSNIIYAAGRPKLADIGLVTTIGEARSFVGTEGFIPPEGPGGPQ
                     ADLFALGKVLYEASTGNDRNDFPNIPQSLVSSADSNDFAELNEVLLRACQEDRARRYA
                     SAREMGEDLILVLQGRSLRRLRSLEKRWEAAKRAMVVAGAAAAIAGLLGYEGYQVSSR
                     RAAIHQRQIGEDIAYGNSALNGGDLLGSLPYFTDVLRLESGKADAEHSDRLRIGSILS
                     QCPKLIHFWSNSPQLSFAEFSPDGAQLLTASIGGGAKLISVDGRAKTRAVGTSMWTKT
                     ATFSHDGRYLAIASQEKTAEIWDANTLALIARLPHPAQVQSARFNAVGTLLVTACSDG
                     AARLFKIGSTNPVLTLQQQKKLWFADFSHDGKWIITAGEDNTARIWSIDGRLKCCCSH
                     PTWVYYASFSPDDSRIVTACADHVARVFDISGQRVLPDLKHDDHIQSAEYSPDGRFIL
                     TASWDSMAKLWRADTLLPADPNPILRNSERLNHAAFSPDGRCIVTAGENGSLKIWDFA
                     GCEAPPRREHCVLSDDGRQKLTIEDASVEVRNVETGELAGPPFSLDGKLEHVQLTRSG
                     RFAIAVQQAEAAGSPKLTILDIHRARAAKQSIPLPSAEARVTASEDGKTAAVYWNKSI
                     QIYDVATAGMIAPKLFFHERIDDLLFSPDGRTLAMQTAAFVRLWDVPTRRILAPSLRC
                     ETTIFHVDFNPQSSEIVVCCRNDLLTACYAQVFDCRTGAALGPRLNHRDGVMAARFDA
                     SGGRIGTAAEDFTAMIWNSSSGQDLIQPVYHKEKVTDVRFGPWGWFATTSDDKTARVW
                     SAQTGDPLTPFLKDLVNLRQVHFSEGGNFLTTTDAKGAVRTWPLRIETMPPKDAVDMA
                     QLLAKGKQDNPWRKTQTSNESPLQAWYRLRTAFPSRFVVSPDEILRWHEVAIEECETN
                     NNWPAAIFHLQALSLLNPTDRSVAVRLRAAQDREP"
     gene            complement(4509..7037)
                     /locus_tag="VG754_13945"
     CDS             complement(4509..7037)
                     /locus_tag="VG754_13945"
                     /inference="COORDINATES: protein motif:HMM:NF022503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF20368.1"
                     /translation="MTKLILKCGLALGDIVMLTAAVRDLHRCYPKEFVTDVRTPFPDL
                     WKNNPWVQRLDDSDTDVWKIDCLYPLLNRCSTTPYHCLHGFIEFLNDKLRLSIKPTEF
                     KGDIHLSTKEKKRPSPVEEVTGQNTPFWIVAAGGKFDVTIKWWSTERYQQVVDKLQGQ
                     VRFVQVGSRGHHHPKLQGTIDLRGLTGIRDLVRLVHHAQGVLCSVTSLMHLAAAVETR
                     HDRPSLRPCVVVAGGREPAHWEQYPGHQFIDTIGTLPCCASTGCWRSRIQPLRDGDIR
                     DQPQRLCGNVVSQLPRCMNMITADEVVRRINYYFEGGLAKRLSSRQARAARQAARLHT
                     PKEFDNQPLTLSSAGMAFDEAAKFIQEKALPEPWQGRGIVLCGGGARYFVNAWVCINM
                     LRHLGCDLPIEVWHLGKREMSKRMASLLEKLDATAVDAEEVRRAHPARILNGWELKPY
                     AVLHSRFEQALFLDADNVPVKNPEYLFKSDAFSKAGAIFWPDFDRPTGNDPGSVWRSC
                     GIRKSREPEFETGQIVLDKNRCWQALRLTMWMNERSDFYYQYLYGDKETFHIAFRKLR
                     QAYALIRKPIHRLEGTMCQHDLSGQRLFQHRNMAKWDLFANRRINGFLYEKKCFSFVE
                     KLREVWDGNVSLNLSLKKKQTPLSQKKPRLGTILLDDSSRTSARGLEKHLRQTDWPTT
                     TPLWRCPDDDLRESLHSALAQALDAGCTSIVLLHGNLIFNRHIYHNLLSWKPFRQGAF
                     DLGSLYDPGVRADALDLPNRARLLRPRRGFSSPAVLISVSFATIFLGQLKAPRTSATD
                     LSMVARTLRQPVHFHAPSLVQFAAPTASLSETPSAFDFDPVWRA"
     gene            complement(7455..8138)
                     /locus_tag="VG754_13950"
     CDS             complement(7455..8138)
                     /locus_tag="VG754_13950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF20369.1"
                     /translation="MQKDVEKPAIDIPTRETLLNRLRDWNDNDSWKVFFDTYWRLIFN
                     CARRSGLTDSEAQDVVQETLISVLKSISDFRYDRSKGRFKGWLCHIANHRIIEHKRSM
                     RRQERLIQPASFPESGEDHLAIAEKASDRIDTEHQKIWDEEWENNFQAAALERVKQKV
                     ELKHFQVFDLCLKQCDPRQIARTLNISRANVYLIKHRIQRMLRTEMSDLLSNPTLEHG
                     NGLKAPNKI"
     gene            complement(8183..8356)
                     /locus_tag="VG754_13955"
     CDS             complement(8183..8356)
                     /locus_tag="VG754_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20370.1"
                     /translation="MSAPTLIALGGDVYTEVLRHMATEDRAALHAMARDFHLTMRSLH
                     NKSFNEMGKSSFF"
     gene            8453..8638
                     /locus_tag="VG754_13960"
     CDS             8453..8638
                     /locus_tag="VG754_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20371.1"
                     /translation="MKDASTLSATAQGWYLPQGRRLTADLSSNRLVKFRFKPFDSPFD
                     SSCFFQLVNRQVMDCAH"
     gene            8750..10024
                     /locus_tag="VG754_13965"
     CDS             8750..10024
                     /locus_tag="VG754_13965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF20372.1"
                     /translation="MNILTHPVHTGYQFHLSQTGHEFYSLEMPGTNEVFWDHASRPNP
                     RNYHRLIHPDEAPVQFDLILAHTVSGFDCLRHFNLPIIYKEHCIRKTFSPPPDWLERV
                     AYYSFASRTAAARWVLPEELAYRKVIIGMGLDVRDFRSGPTKLGRILCVVQNASSRGE
                     EKGLHNLVPLAQILPITTVGRGNDNIPGAVGPARNYDELRRYYASHSVFFNPSSILGL
                     STLEAMAAGMAVVSFRTINSDIIEHGRNGLVVNSVRDAGMALRKLLRSRELAGHLGRN
                     ARRTIQERFSPKLFCARWNALFRLAAFEYHADFEPKLWRKFEIDEKPEPIRQAARELT
                     RGTFEYHRIGYDKRHMTFLESGWVGEGRRARELFWDIKAGSTGPYLELSSGVSLTCRL
                     RRRRDGTWTGRWLHCEKMPIMVRPLAQSVTHT"
     gene            complement(10011..10709)
                     /locus_tag="VG754_13970"
     CDS             complement(10011..10709)
                     /locus_tag="VG754_13970"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF20373.1"
                     /translation="MIAKIFALIAFRDEEQHLPGLLSHLRDYVDGFVGFDDCSLDHSA
                     KIARGEPKMLEVLQRKTPSADHRFEIENRRDLLKTATKKGADWVLCCDADERFETRLL
                     EDLRKLIAEPPAEVLGLPLVAVWENFHQHRVGKAKKFVLFRSEDPDPYYCPGSLHQQW
                     FPPRYYSRSQQLLDYYIYHLGSITRADRLARYEKFNRIDPDKRHQPLGYENIIDETNV
                     ILNEIKPERSFKYA"
     gene            complement(11191..12567)
                     /locus_tag="VG754_13975"
     CDS             complement(11191..12567)
                     /locus_tag="VG754_13975"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWF20374.1"
                     /translation="MVTAANAFATSIPARLEKEMNPTRSEASPALVCWSADTEADEAT
                     LLAQQVKNLHQQGRPYSDFAVLLRSVKTSAPAIVAAFEAEDIPFNCGGRTGLFLNPEI
                     DALGELFAWISDSNWRDGGRFARTIGTSPYRPATEAGPAQVLGQAFGLSKTETEEFIL
                     YLRDWKKHRLSSAGKGRVSFVGDLYRILDTLGIWKLRPDEKPKDSSRLGAFARFSKIL
                     VDFESIAFRGRKEAVAPAKAGATDFKFAPAVGTAEARNKIVWGSLANFQLGYAKDNYE
                     DFEGETSSDRDEVSIFTVHQAKGLEWPIVFMPSLTSRRFPSSMTGRAQTWLLPDNCFG
                     ADQRARYEGSDADERRLFYVALTRACDAAYLSHARRKTNKMEPSPYLQEVAGSKIIPL
                     LTELPKPAVTDETAPKHEPVELSFSDLADHEHCGHAYRLSRVFGFQREVADELGYGRA
                     VHHVMRTL"
     gene            complement(12507..13010)
                     /locus_tag="VG754_13980"
     CDS             complement(12507..13010)
                     /locus_tag="VG754_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWF20375.1"
                     /translation="MLTEHKIELGNHDVLDEHRHQAFVHRYRKALDLETLARNATGRK
                     VPGFEAAALFISAVDALGNDLIPETALAGSPFAPAYRKYRELLDQHRFLTFGLIISEA
                     VHWLENAPAFRASVRRDLSHLLVDEYQDVNPAQERLISTIGVAALESSQPPTHSPRPS
                     RHGWKRR"
     gene            complement(13004..13333)
                     /locus_tag="VG754_13985"
     CDS             complement(13004..13333)
                     /locus_tag="VG754_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWF20376.1"
                     /translation="MSLSDPQIYEIGFSPTPGQRTIIENHDGDLQVIACAGSGKTESI
                     SRRVAEILRRGAKPEAIVAFTFTEKAAFELKERVTKHVRRVLGEEALGNLGRLYVGTS
                     TATAIAF"
BASE COUNT         3090 a         3648 c         3518 g         3184 t
ORIGIN      
        1 ggatgctctt ctccccgcga tccgcctcgc gcaaaagccg tatcttctct tctgtcgtat
       61 accgtttgcc tttcattctg ggtcctttct tggcccccag actaacacct cgcccggccc
      121 gaaaaagcgc agtcacgtca ggagttcatc gccaagctgg tgcagcaatg gctggcggct
      181 aaccagatca aaaccatcta catcgagccc ggcagtccct ggcagaacgg ctttgtggaa
      241 agcttccacg gccgcttccg cgatgaatgc ctgaatcgcg agcaactctg gaccctgacc
      301 gaggcccccg tggtggtggg cgactaccgc caagaataca accaattgag gccgcatagc
      361 cggctcggct atgaaacccc tgccagcttt gccgcccgaa cctgtccatc cccgtctccg
      421 atcgggcttc gccctccctc cgacaaggat ggacaaacca cccagaacac cacaacatga
      481 atcaagcccc tagactaaca ctccagtggc ccagaaaagc gcgcccggtc aggaacgctt
      541 tacagcccag tattaaagag cggccctttc ggaggtatcg acgcgaggaa cagcttgatg
      601 cggaaatgat actgtatgcg acggggttgc tcttgcatcg cgatgggaaa ccgaaccaat
      661 gttttccatg aggtcggaaa tcgatgtgct aaggccttct gcaagcctaa acaaggaatg
      721 aaagcttaca tttcggacgc ctctctcgat gtcgcagaca taagttcgat gaaggcccgc
      781 cttctcggcg aggttttcct gggaaagtcc tgaacgcttt cttcgatcac ggatattgcg
      841 gcctaaagtc ttaagctcgg ggatcaatga gaaacacgat tctggaatgg aaatcgcggc
      901 tcgctttcga gttgtttcat gaggcataat ttaacgcttc atactttatt gcgtttgtaa
      961 tcgagcagat ctttcagaag atcttacaat tccaaattcc actcaaaagg tgaggtgcca
     1021 gaagactcaa actgacggcc ccttggaact gttagggctc tcgatcttgg gccgcccgca
     1081 agcgaactgc aactgatcgg tctgtgggat tcaagagaga cagcgcctgg aggtgaaaga
     1141 tggctgccgg ccagttgttg tttgtttcgc attcctctat ggcgacctcg tgccatcgaa
     1201 gaatctcatc gggtgatacg acaaaccgcg atggaaatgc agtgcgcaac cgataccatg
     1261 cctgcaatgg cgactcattc gaggtttgag ttttccgcca tgggttatct tgtttccctt
     1321 tcgccagcag ctgtgccatg tcgaccgcat cttttggggg cattgtttcg atacgcaacg
     1381 gccacgtcct gactgcaccc ttggcatctg ttgtcgtcag gaagttgccg ccttccgaga
     1441 aatgcacctg ccgcaggttg actaaatctt tgaggaaggg ggttaaggga tcgccggttt
     1501 gcgcactcca aactcgtgct gttttgtcgt cgctggtcgt tgcaaaccat ccccaaggac
     1561 cgaatcggac gtcagtgact ttttctttgt ggtacaccgg ttggattaga tcttgaccgg
     1621 aagaggaatt ccagatcatg gcagtgaaat cctctgcggc agtgccgatt cgaccgcccg
     1681 aagcatcgaa gcgcgctgcc attacgccat cacggtgatt aagcctcggc ccgagggccg
     1741 caccagtgcg gcagtcgaac acttgagcat agcaggcggt gaggagatcg ttacggcaac
     1801 aaacgacgat ctcggaactc tgcggattga aatcgacgtg gaaaatcgtc gtttcacatc
     1861 gtaaagaagg ggcaaggatg cggcgtgtgg gaacgtccca taagcggacg aaggcggccg
     1921 tttgcatcgc cagggttcgc ccatcggggc taaagagcaa gtcatcaatt cgctcatgaa
     1981 agaacagctt cggagcgatc atcccggcag tggcaacatc gtaaatttgg atagatttgt
     2041 tccaatagac tgctgccgtc ttaccatctt cgctcgcggt caccctggcc tctgcgctgg
     2101 gtaatggtat ggactgtttc gcggcacgag ctctgtgtat gtcgagaatt gttaatttag
     2161 gactgccggc tgcctctgcc tgttgaacgg ctatggcgaa acgaccggat cttgtcagtt
     2221 gcacatgctc tagtttgccg tcgagagaga atggagggcc cgccagctcg ccagtctcaa
     2281 cattcctaac ttcaacgctc gcgtcttcaa tggtgagctt ctgccggcca tcgtcgctca
     2341 aaacgcagtg ttctcgccgg ggaggggcct cgcatccagc aaagtcccag atttttaggc
     2401 taccgttttc cccagcagtc actatacagc gcccatcagg gctgaatgcc gcgtggttga
     2461 gacgttcgga atttcggagg atgggattcg gatctgcggg aagcagcgta tctgctcgcc
     2521 aaagtttggc catgctgtcc cagcttgcag ttaggatgaa gcgtccatca gggctatatt
     2581 ccgcgctctg aatatgatca tcatgtttca gatcaggcaa aacgcgctgc ccggatatgt
     2641 caaacactcg tgctacatga tccgcgcatg cggtcacaat tcgtgaatca tctgggctga
     2701 aggaggcgta gtaaacccat gtgggatggc tgcaacagca cttcagccgt ccgtcgatgc
     2761 tccagatcct tgccgtgttg tcttcaccgg cggttatgat ccacttccca tcgtgactaa
     2821 aatccgcgaa ccaaagtttt ttctgttgtt ggagtgtcaa taccgggttt gtgctcccaa
     2881 tcttaaaaag acgtgccgcg ccatcgctgc aagcagtgac caacagagtg cccactgcat
     2941 tgaatctggc gctttggacc tgggctggat gaggaagtcg agcgatgagc gcaagcgtat
     3001 tggcgtccca aatctccgcc gtcttttcct ggctcgctat tgcaaggtat cggccgtcat
     3061 ggctgaatgt tgctgttttt gtccacatcg aagttccaac agccctcgtt tttgctcgac
     3121 cgtcgacgct aatcagcttt gccccacccc caatagaagc ggtcagaagt tgcgctccgt
     3181 cgggactgaa ttcagcaaaa ctgagctgtg gactgtttga ccagaaatgg atgagtttag
     3241 gacattgact gagaatcgat ccgattcgca agcgatcgga gtgttccgca tcagctttgc
     3301 cggattccaa gcgcaaaacg tccgtgaaat acggcaatga gcccaagaga tctccgccat
     3361 tcaacgcgct gttcccgtag gctatatcct cgccaatttg gcgctggtga atggctgccc
     3421 gacggctcga aacctgatag ccttcgtacc ccagcaagcc cgcaattgcc gccgcggcgc
     3481 ccgcgacgac catggcccgt tttgcggctt cccatcgttt ttctaaggat cgaagccgtc
     3541 gcaacgaccg gccctgaaga acaagaatca agtcctcacc catttctcga gcagacgcgt
     3601 aacggcgggc gcgatcttcc tggcaagctc gcagcaaaac ctcattcagc tccgcaaagt
     3661 catttgaatc agctgaacta accaatgatt gagggatgtt tggaaaatca tttctatcgt
     3721 tgcctgtgct tgcctcatag agaaccttgc ccagcgcgaa taaatctgcc tgcggtcctc
     3781 cgggcccttc cggtggaata aatccttccg tgccgacaaa cgaacgtgct tctcctatgg
     3841 ttgtcactag gccaatgtcc gcgagttttg gccttccagc ggcgtaaatg atattggacg
     3901 gcttgacatc tcggtgcacc aagccctcgt cgtggattct ttccagggct ccgcacattg
     3961 ccaaaccaag atcggcgcac tctcgcacgg acaatcgact gcggcgagag atttcgcgcc
     4021 gcaaagtccg gggcgaataa ctatccggcc gaatcgcttt tcccaattcg acatcatccc
     4081 ccaactccat tacataataa aaacaattgg cggcttgatc gattcccaca tggaggatat
     4141 ccacgaatcc gtcatgggaa cgggatatcg gttcgaattg gcgaatgccg agcagctccc
     4201 gttcaaatgg acggtttgtc tcaaatgaac ttcgactgac aaccttgacg gcgcggaaca
     4261 tcccgaggca gtgctgcgcc agccacacct cgccatagct gcccacccca atgcatcgaa
     4321 gcattttatg atcaggaata acagggggcc ccggcgacag tgctctgccg cggatcaccc
     4381 cggatcgtga aaggccggaa tcggttgata ttgggcggcc catgcttgca agtcgccacc
     4441 agtaaatcgg atgccggaat tttcggcaat ggaattgtca cccaaaggca acaagcacaa
     4501 gaatcgcctc aagcccgcca aacggggtca aaatcgaaag ccgagggtgt ttcagagagc
     4561 gatgcggtgg gcgccgcaaa ctgcaccaac gagggagcat gaaaatgcac tggctggcgc
     4621 aatgtgcggg caaccatcga taaatcggtg gctgaagtgc gcggagcttt caattgtccc
     4681 aggaaaatgg ttgcgaatga caccgaaata aggacggctg gcgaagaaaa tcccctgcga
     4741 gggcgcagca accgcgctcg attaggaaga tcaagtgcat cagcgcggac tccgggatca
     4801 taaagacttc caagatcaaa tgctccctgc cgaaacggtt tccacgaaag caggttatga
     4861 tagatgtgac gattgaagat gaggtttcca tgcagcagga cgattgaagt gcagccggcg
     4921 tccaacgctt gagccagggc ggaatgcaac gattcacgca aatcgtcgtc tggacaccgc
     4981 cataacgggg tggtcgttgg ccagtcagtc tgcctcaaat gtttttctag gccacgagcc
     5041 gatgttctgc tgctgtcatc cagcaggatt gtccccagtc tcggtttttt ttggctcaac
     5101 ggagtctgct ttttcttcaa agacaggttc aaactgacat ttccgtccca aacctctctg
     5161 agcttttcca cgaacgagaa acacttcttt tcataaagaa atccgttgat gcgccggttg
     5221 gcaaataagt cccatttagc catgtttcga tgttgaaaga gcctttggcc ggagaggtca
     5281 tgctggcaca tggttccctc cagccgatga atcggcttcc gaatcagagc ataggcctgg
     5341 cgcaattttc gaaaggcaat atgaaacgtt tccttgtccc catacagata ttgataataa
     5401 aaatccgaac gttcgttcat ccacatggta agtcgcaaag cctgccagca acggtttttg
     5461 tcaagaacta tttggccggt ctcaaattct ggctcgcggg atttcctgat tccgcagctt
     5521 ctccaaaccg atcctggatc gttcccggtt gggcgatcaa aatctggcca aaaaatagct
     5581 ccggccttgc tgaaggcgtc agatttaaac agatattccg gattcttgac cggcacattg
     5641 tctgcatcta ggaaaagcgc ttgctcgaaa cgggaatgga gaaccgcgta aggcttcaat
     5701 tcccaaccat tcaggatgcg ggctggatga gcgcgtcgca cttcttccgc atcaacagcc
     5761 gtggcgtcga gcttttccag aaggctggcc atccgtttgc tcatttccct ttttcccaga
     5821 tgccaaactt caatgggcaa gtcgcatcca aggtggcgca gcatattgat gcacacccaa
     5881 gcgtttacaa aatatcgtgc ccctcctccg caaaggacaa tgccccgccc ctgccatggt
     5941 tcgggcagcg ccttctcttg gatgaatttt gcggcttcat cgaaagccat accggcgctt
     6001 gaaagagtta aaggctggtt atcaaattcc ttcggcgtgt ggagtcgggc tgcttgccgc
     6061 gccgccctgg cttgtcgaga cgacagacgc ttcgccagcc caccctcgaa atagtagttt
     6121 attcgtcgca caacctcatc tgctgtaatc atattcatgc agcgcggaag ttggctaacc
     6181 acattgccgc ataaacgttg cggctggtcc cgtatgtctc catcacgcaa cggctgaatc
     6241 cggctgcgcc agcagccagt tgaagcgcaa cacggcaagg ttccaatggt gtcaataaat
     6301 tgatggcctg gatattgttc ccagtgcgcc ggctcacgcc cgcccgccac cacgacacag
     6361 gggcgcaaag acggccggtc gtgtcttgtc tccacagctg cagccaaatg catgagggaa
     6421 gttacagagc aaaggacgcc ttgagcatga tggaccagcc gaactaaatc tcggatcccc
     6481 gtcagacctc gaagatcaat cgttccctgc agcttgggat gatgatgtcc tcgtgaacca
     6541 acctgaacga atcggacttg gccctggagc ttatcgacaa cctgctgata acgctccgtg
     6601 ctccaccatt taatggtgac gtcgaatttc cctccggcgg ccacaatcca aaatggcgtg
     6661 ttttggccgg tgacttcttc aacaggcgat ggcctctttt tctcttttgt cgaaagatgg
     6721 atgtcgcctt tgaattcggt cggtttgatc gagagacgca acttgtcgtt tagaaattca
     6781 atgaatccat ggaggcaatg gtacggcgtc gtgctgcacc ggttcagaag cgggtacaaa
     6841 caatcgatct tccaaacatc tgtatcggag tcgtcaagcc gttgaaccca cggattattc
     6901 ttccacagat ctggaaacgg cgttctgacg tcggtgacga attccttggg atagcaacgg
     6961 tgcagatccc gcacggccgc cgtcagcatc acgatatccc ctaatgccag accgcatttg
     7021 aggatcagtt tcgtcataag agaccgtcca gccccggagg caggggccaa cggttgactg
     7081 cgctgtgtgg atggcaatca gcgtttctgc gttgccgtag ccacgctttt tctgcgcacg
     7141 ctatcctctg gagggctcac tggttcctga ctaggggagg gcgaattcgc catctgcggc
     7201 attccggttg aattggcgcg accgtttctg acggatttag gtgtgggaat tgtcgtgaac
     7261 atagttttgt taatctggct ctgatttatt tgtattggaa gaacagattt tttgaaattt
     7321 cggttgacat ggcaacatgc cgtccgcgat taaacggtct ctaaagcggc gcaataaccg
     7381 ccggcgaatt ctttgcatca ttcgcgcagc gcccgcctgc ggacaggggg attgatgacg
     7441 cggaaaccaa ggcttcatat tttattcgga gcttttaatc cattcccatg ttcaagcgtc
     7501 ggatttgaaa gcaaatcgct catttccgtc cgcagcattc gctgaattcg atgttttatc
     7561 aaatagacat tggctctcga tatattcagc gttcgcgcaa tctgccttgg atcgcattgt
     7621 ttcaaacaca ggtcgaatac ctggaagtgc ttcaattcga ctttttgttt caccctttca
     7681 agggcggccg cctgaaaatt attttcccat tcctcatccc aaattttctg atgctccgtg
     7741 tcaatccgat ctgacgcttt ctctgctatt gccagatgat cctccccgct ttccgggaag
     7801 ctagccggct gtatgaggcg ctcctggcgt ctcatagatc gtttgtgctc tataatgcgg
     7861 tggtttgcga tatgacaaag ccaccctttg aaacggccct tgctgcgatc atagcggaaa
     7921 tcggatatgc ttttgagaac gctaatgagg gtctcctgca ccacatcctg cgcctctgaa
     7981 tcggtgagcc ctgagcgacg ggcgcaatta aaaatcagcc gccaataagt gtcgaaaaac
     8041 accttccagc tgtcgttgtc attccaatcc cggaggcgat tcagcaacgt ctctcgcgtc
     8101 ggaatgtcga tcgcaggctt ttcaacatct ttctgcacac tggttaatgc gatgcagtcg
     8161 cataaaatct ttcatgccgc cactaaaaaa atgaactttt tcccatttca ttgaaagatt
     8221 tgttgtgcag cgagcgcata gtcagatgga agtcgcgcgc catcgcgtgt aaagccgcac
     8281 ggtcttccgt cgccatgtgg cgaagaactt cagtgtaaac gtccccgccc aaggcgatca
     8341 gggttggtgc gctcatggac gcgagcattg cagagggcaa gggaggccgt cggccatcgg
     8401 cggcctctct tgagggttcc agcgcctttt ggcgagctgt ggatcactgg ggatgaaaga
     8461 tgcctcaact ctttctgcca ccgcccaagg ttggtatctg ccgcaaggac gccggctgac
     8521 tgcggatttg tcttcgaacc gtttggtcaa gtttcgattc aagccgttcg attcgccttt
     8581 tgatagcagc tgcttttttc agctggtcaa ccgtcaggtg atggattgcg ctcattgaac
     8641 catttctcca aatgttggcc gggcaatcaa caaaaatgcg ctacgcctca ttgccagttc
     8701 ggctcgcctt ttgggcggat ttgacatatt catttgtctt tggtgcaaca tgaatatact
     8761 gacccatccg gtgcatactg gttaccagtt tcatctttca caaacgggcc atgaatttta
     8821 ttcgttggaa atgcccggaa ccaacgaagt cttctgggat cacgcttccc ggccaaatcc
     8881 acgcaattat catcgcctaa ttcacccaga cgaagctccc gtccaatttg accttatcct
     8941 ggctcacaca gtgtcgggtt tcgactgcct gaggcatttc aatctgccga ttatctataa
     9001 ggagcactgc atcagaaaaa cattctcacc gccgccagac tggctggagc gggtcgcgta
     9061 ttattcgttt gccagccgta cagcggcggc gcgttgggtt ctacctgagg aactggctta
     9121 tcgaaaggtg atcatcggca tggggctgga tgtgcgcgat ttccgaagtg ggccgaccaa
     9181 actaggccga attctttgcg tcgttcaaaa cgcatcaagt cgcggagagg agaaggggtt
     9241 gcacaacctc gtccccttgg cccagattct cccgattaca actgttggcc gtggaaacga
     9301 caacattcct ggggccgtcg gcccagcccg taattacgat gagcttcgac gttactacgc
     9361 gagccatagc gtttttttca atccatcgag tatcctaggc ttatcaacct tggaggcaat
     9421 ggccgccgga atggcggtcg tttcattccg gacgataaac tcagatatta ttgaacatgg
     9481 acgcaacggg ttggtggtga attcggtaag ggacgctgga atggccctca ggaaacttct
     9541 tcgaagccga gaactggcag gccatcttgg aagaaatgca cggcgtacga ttcaggagcg
     9601 cttctcgccg aagctgtttt gcgcacgttg gaacgccctt tttcggctcg cagcctttga
     9661 ataccatgct gatttcgagc cgaaactttg gcgcaaattt gagattgatg aaaaacctga
     9721 gccaattcgc caggcggcga gggaattgac acgtgggact ttcgaatacc atcgcatcgg
     9781 ctatgacaaa cgccacatga cgttcttgga aagcggttgg gttggggaag gccgccgtgc
     9841 cagggaacta ttctgggaca ttaaagcggg ctcgactggg ccctaccttg aactttcttc
     9901 aggcgtttcg cttacctgcc gcctaagacg ccgacgcgat ggaacatgga ccggacgctg
     9961 gcttcactgc gagaagatgc ccatcatggt gcggcctctg gcccagtccg tcacgcatac
    10021 ttgaaagatc gctccggctt aatttcattc aagatcacgt ttgtttcatc gatgatgttt
    10081 tcgtagccga gcggttgatg acgtttgtct ggatcgatcc ggttgaattt ctcgtatcgg
    10141 gcgagccggt cagctcgcgt gatgcttcca agatgataaa tgtaatagtc aagcaactgt
    10201 tgcgatcgtg aataataccg gggcgggaac cactgttgat gaagcgagcc agggcaataa
    10261 tacgggtcag gatcctccga gcgaaacaaa acgaattttt tcgccttgcc aactcggtgc
    10321 tggtgaaaat tttcccagac agctacaaga ggcaagccca agacttcagc cggcggttcg
    10381 gcgatcagtt ttcgcaggtc ttccagaagc cttgtttcaa accgttcatc ggcatcgcag
    10441 catagcaccc agtccgctcc cttcttagtc gcggtcttta agagatctcg gcgattttcg
    10501 atctcaaacc gatggtctgc actcggcgtt tttcgttgca acacctccag catcttaggc
    10561 tctccgcggg caattttagc cgaatggtct aatgaacagt catcaaaacc gacaaacccg
    10621 tccacataat cgcgcaaatg tgacaagagt ccgggaagat gctgttcctc atcccgaaac
    10681 gctattaacg cgaaaatttt tgcaatcata aggcggcaaa ccgcgtgttt tatctcctgc
    10741 cagcatcatt ttcgatgcga gctccaactc ccataccgag caactgcccg tccatcaagc
    10801 gcaattcgag cgttgagtga cccaaatttt caatcctcac ttatgagcaa acaaatcatt
    10861 cgagtctggc acttcgtgtc cgactccaat tcagacattc aatacgaaac cctgcaattc
    10921 atcgacggct caacctcctg taattgcaag ggctggactc gtcgtgtggc cccagatggc
    10981 agccgatcct gcaaacacac ccgcttcgtc gacatggggc tggcggatca atactgcaca
    11041 gccacacacg attacgaaac tcaccaaaat cacgaacctc aacgaaagga accacaaaat
    11101 gccaaagtct caaaggtctc aagtctcccc aagctcggac atcgcaaatt cgccgtctga
    11161 gcccaacaac atgcgaggca gaattatcaa ctacaaagtt cgcatgacat gatgcacggc
    11221 acggccgtat ccgagttcgt cagcgacttc ccgctggaag ccgaacacac gggacagccg
    11281 ataggcatgt ccacagtgtt catgatcggc aagatcggag aacgacaact caaccggttc
    11341 gtgctttggc gcggtttcgt ccgtgaccgc tggctttggc aattcagtca gtagcggaat
    11401 aattttggag cccgcaactt cctgcaagta gggcgagggt tccatcttgt tggttttccg
    11461 tcgcgcatgg gagaggtagg cagcatcaca ggcgcgtgtc agtgccacat aaaacaaccg
    11521 acgttcgtcc gcgtcactgc cttcgtaacg ggcacgttgg tcggccccaa agcaattgtc
    11581 ggggagcagc caggtttgcg cccgtccggt catgctggaa gggaaacgtc ggcttgtcag
    11641 gctgggcatg aacacgatgg gccattcaag gcccttggct tgatgcacgg tgaaaatgga
    11701 aacttcatcc cggtcactgg atgtttctcc ctcaaaatcc tcgtaattat cttttgcgta
    11761 accgagctgg aaatttgcga gtgaacccca tacaatcttg ttgcgagcct cagcggtgcc
    11821 aaccgcagga gcgaacttga aatctgtcgc ccctgctttg gcgggagcta cggcctcttt
    11881 ccgtccccga aaagcgattg actcaaaatc tacgagaatc ttggaaaagc gggcaaaagc
    11941 cccaagccgg gaagagtcct tcggtttttc atcgggtcgc aatttccaga taccgagcgt
    12001 gtcgagaatg cggtagaggt cgccaacaaa agacacgcgg cctttcccgg cactggagag
    12061 gcgatgtttt ttccaatcac gaagatagag gataaactcc tcggtttcgg ttttggaaag
    12121 gccgaatgct tggccaagaa cctgggctgg cccggcttct gtcgccgggc ggtaagggct
    12181 ggttccgatg gtgcgggcaa accgcccccc gtcgcgccag tttgaatctg agatccaagc
    12241 gaacaattcc cccaaggcat caatttctgg attcagaaaa aggccggtgc gtccgccaca
    12301 gttgaaagga atgtcctcag cctcaaacgc ggcaacaatg gcgggagccg atgttttcac
    12361 cgaacggagc aaaacagcaa aatctgaata gggcctgcct tgctgatgaa ggtttttgac
    12421 ctgctgggca agcaaggtgg cttcgtcagc ttccgtgtcg gcggaccaac aaaccagtgc
    12481 tggcgatgcc tcagacctgg ttggattcat ctccttttcc agccgtgccg ggatggacgt
    12541 ggcgaatgcg ttggcggctg tgacgattcg agggcggcta cgccgattgt cgagattaac
    12601 cgctcctggg ctgggtttac gtcctgatat tcgtccacca gcaaatggct gagatctcgc
    12661 cgaacggatg ctcggaaggc aggtgcgttt tccagccagt ggactgcctc ggaaatgatg
    12721 agaccaaaag tcaggaaccg gtgctggtca aggagttcgc ggtattttcg ataagcaggg
    12781 gcaaaaggac tgccagcaag ggcggtttcg ggaatgaggt cattgcctaa agcatcaaca
    12841 gcggaaatga acagagccgc cgcttcaaag ccgggaactt ttctgcccgt ggcgttgcgg
    12901 gcaagggttt ccaaatctag cgctttccgg taacggtgga caaaggcttg gtgccggtgc
    12961 tcgtccagaa cgtcatggtt gcccagttcg atcttgtgct cggttagaat gcgatagcag
    13021 tagccgtgga ggttcccacg tagagtctgc caagattccc cagcgcctct tctcccagta
    13081 cccggcgaac atgcttagtg acgcgttcct tcagttcgaa cgcggctttt tccgtgaacg
    13141 tgaaggcgac gatggcttcc ggttttgcgc cccggcgaag tatttcagcc acgcgacgcg
    13201 agatggattc cgttttgcct gagcctgcac atgcaatgac ctgcaaatca ccatcgtgat
    13261 tttcaatgat cgtccgctgc cctggcgttg gactgaagcc aatttcgtag atttgaggat
    13321 cagaaaggct catgtatctg tgcagtgcta accatgtacc tcagtgtttt gcagggggtg
    13381 taaaagtctt tctgtaaatt gtctttcttc catccgcctt ggcccctttg gggtgaggcg
//
LOCUS       DASZMM010000582         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2464932, whole genome shotgun sequence.
ACCESSION   DASZMM010000582 DASZMM010000000
VERSION     DASZMM010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464932"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VG754_13990"
     CDS             <1..180
                     /locus_tag="VG754_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWF20377.1"
                     /translation="YKGNIIVTGRKSPVSLYNPQIATMEADPTKAYNQDDATGFIRLN
                     GLRLKVASKVHRIKK"
     gene            182..1051
                     /locus_tag="VG754_13995"
     CDS             182..1051
                     /locus_tag="VG754_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20378.1"
                     /translation="MKKSTIICSLIGLALALHAQDAKQPTAIRATVGDVTDNRTTGTF
                     NSECKIEVKFTGDAAADATDVHEVRVTKAVDELGRDLVPKEKPDHFGGHMSNFHHRQN
                     GLTAEVVLRNPSRSSSVIKLVEGEVEFFNPTASNGGILIIKNILAHPAEAVQDATLKK
                     YGVEVMYLTKASYEAKKKQIQDEEKSGSGGAIGQAFGDLFKGMFGGMMSSDSKDSIKL
                     YIKDTDKRVVDFEFQDANGKPLKSHGSWSMSEMHNQDLDAPPPADTQLLIHLATPEAT
                     QTFPFKLENIPLP"
     gene            1117..1959
                     /locus_tag="VG754_14000"
     CDS             1117..1959
                     /locus_tag="VG754_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20379.1"
                     /translation="MKFYNLENAPMDRKDLVFRKSRVMAAVPAIICLGIALMALLVAL
                     KIINVPKGFPAVIACLIAVFMGLIGLAFFAQFRSTLKPTNWLLRANQGGVLIKYRSFL
                     NRKLPATDLQVVGIEYSEIDWARKAIEKRTSLGMGNANSGKSETLKYLDFGLLVETSA
                     LAQNLQAENRSESAASSHWEDFPVQLMDGGIVRIKWNGVVPHLRCALKEIERHAKIQD
                     AESSSTDYVTARNLAPDELKAKVLKLAASGETMAACELARKALRCSLTEAVQYVEKVK
                     LESV"
     gene            2109..>4159
                     /locus_tag="VG754_14005"
     CDS             2109..>4159
                     /locus_tag="VG754_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWF20380.1"
                     /translation="MEGATAQEIETGGSKSFADTQSVSPLDSFTGIITPQPVSPLYKI
                     GLAVVAFAMVLLPAVYGFFILLAGWTVVLLVKNDFSDGGTGKLTFYLGPACGILIFFM
                     LKPLFAKRPVRVQAPTLDPAKEPLLFAFVQRICEQVRAPMPSRIDVDCQINASASLRR
                     GFWSRDLVLTIGLPLAAGLDMREFAGVLAHEFGHFAQGAGMRLTYIIRNINFWFARVV
                     FERDQWDQHLERWSKRLDIRLAIVLHAARLCVWLTRRILWLLMHAGHGISCFMLRQME
                     YDADSYETKLAGSDAFESTATRLRELNAAMQLAVEEVRQDWASRRLPENLPLLVQHKA
                     ASLPAELQQKYVGAADSKKTGWFDTHPCDSDRVKAARALAQPGVFRIERPATELFADF
                     NETGKAVTRFQYEKHWKLEFSAQNLVASEEMLRESAVNAEADAAIRKFYGEVNITLAP
                     LLNDAQLPPLTDRDTAMTQWREAREESLRLRPQAEKASGECLVQQARLVQMMGAWSLS
                     RAGFKLEAKAFGFPASATSVADQEIAGKFAVEEASGTIRQQIQMLNPFVAALRTRITL
                     ALQLTRPAATNGEPASELDKLIKALAATGKTLPTLHEIGIRLRPFTVLAQNRGNHRNP
                     MEVSSHMNRIADELQKLAAEAQLPLGGLAYPFPHPRGQLSVIEYARYEKPTQNRWEEA
                     Y"
BASE COUNT          993 a         1109 c         1169 g          888 t
ORIGIN      
        1 tacaagggca acatcatcgt gacgggccgg aaatctccgg tcagccttta caatccgcaa
       61 atcgccacga tggaagccga cccgaccaaa gcttataacc aggatgacgc gacgggattc
      121 atccgcttga atgggttgcg gttgaaagtt gcgtcgaaag ttcatcgaat aaagaaataa
      181 catgaaaaaa agtaccatca tttgttcgct aattggactg gcacttgcgc tccatgcaca
      241 agacgcgaaa caaccgactg ccattcgcgc cacggttggt gatgtgaccg acaatcgcac
      301 caccggcaca ttcaattcgg aatgcaagat cgaggtgaaa ttcaccggtg atgccgcggc
      361 ggatgcaacg gatgttcatg aagtgcgcgt caccaaggcc gtggacgaac tcggacgcga
      421 tttggtcccc aaggaaaaac cggaccattt cggcgggcac atgtccaact ttcatcatcg
      481 ccaaaatggg ctcactgccg aggtcgtatt gcgaaaccct tcgcgcagct cgtcggtgat
      541 caaactggtc gaaggcgaag ttgaattttt caatccgacc gcgtcgaacg gcggcattct
      601 catcatcaaa aacattctcg cgcatccggc cgaggcggtc caggacgcga cgctcaaaaa
      661 atatggcgtc gaagtgatgt atctgaccaa ggcaagttac gaggcgaaga aaaagcagat
      721 ccaggatgag gaaaaaagcg gttcgggcgg cgcaataggg caggcattcg gcgatttatt
      781 caaaggcatg ttcggcggca tgatgtcctc tgattccaag gacagcatca aactgtatat
      841 caaggacacg gacaaacgcg tggtggattt tgagtttcag gatgcgaacg gcaagccgct
      901 caagagccat ggcagttgga gcatgagcga gatgcacaat caggacttgg acgcgccgcc
      961 tcccgcagac acgcaattgc tgattcatct ggccacgccg gaagcgacgc aaacttttcc
     1021 gttcaagctg gaaaatattc cgcttccttg agcggagcga aatgcgttat cgaaacgcga
     1081 aaaagaccgg caacgcaagg tcatccgttt ggagccatga aattctacaa tttagagaac
     1141 gcgccgatgg accggaagga tttggttttt agaaaatccc gtgtcatggc ggctgttccg
     1201 gcaatcattt gcctcggcat tgcattgatg gcgttgctgg tcgcgctgaa aatcatcaat
     1261 gtccccaagg ggtttccggc ggttatcgct tgcttgatcg cggtattcat gggattgatc
     1321 ggcctcgcat ttttcgcgca atttcggtcc accctgaagc ccacgaactg gctgctgcgc
     1381 gcaaaccagg gcggcgtgct gatcaaatat cgttcctttt tgaatcgcaa attgccggcg
     1441 acagatctcc aggtggttgg aatcgaatat tcggaaatcg actgggccag aaaggcgatt
     1501 gaaaagcgga cttcattggg catgggtaac gccaattccg gcaaatctga gactttgaaa
     1561 taccttgatt ttggcctgct tgtcgaaaca tccgctcttg cgcaaaattt gcaggcggaa
     1621 aaccgcagcg aatccgccgc gagttcgcat tgggaagatt tccctgtcca gcttatggat
     1681 ggtgggatag tccgcattaa atggaatggc gtcgtgccgc atcttcgctg cgcgcttaaa
     1741 gaaattgagc gtcatgcaaa gatacaagat gcggagagtt ccagcaccga ttacgtaacc
     1801 gcgcgcaacc tggctccgga cgaactgaaa gcgaaggtct tgaaactcgc cgcgtccggc
     1861 gaaacaatgg ccgcctgcga actggccaga aaagcattgc gctgttcttt gaccgaagcg
     1921 gttcaatatg tcgaaaaggt gaagttggaa agcgtgtgaa cggtgtttgg gtcgcagtcc
     1981 gtgatcgtgg attccagggc atgaaatttt atgcgctcgc gttgaatcgg cttgcctgaa
     2041 attttgtttc gggatttact gtttgcgcgt cggaacatcc gaaaactcgc tgttgagaat
     2101 aaaagattat ggagggtgca accgcacaag aaattgaaac cggcggctca aaatcattcg
     2161 ccgacacgca atccgtctct ccgctcgatt cgttcaccgg catcatcact ccgcaaccgg
     2221 tttcgccgct ttacaagatc ggactcgcgg ttgtggcatt tgcgatggtc ctgctgccgg
     2281 cagtttacgg cttcttcatt ctgctggcgg gatggactgt ggtgttgctt gtgaaaaatg
     2341 atttctcgga cggagggacc ggcaaactga cgttttacct cggcccggcg tgcggaatct
     2401 tgattttctt catgttgaaa ccgctgttcg caaagagacc ggtgcgggtt caagcgccaa
     2461 ctctcgaccc cgccaaagag ccgctcctct tcgcatttgt gcagcgcatc tgcgaacaag
     2521 tccgcgcgcc gatgccctcc cgcattgatg tggattgcca aatcaacgcc tctgccagcc
     2581 tgcggcgcgg tttttggagc cgggatttgg tgctcaccat cggcctgcct ttggcggcgg
     2641 gattggacat gcgggaattt gccggtgtgc tcgcgcatga attcggccac ttcgcccaag
     2701 gcgcgggaat gcggcttact tacatcatcc gcaatatcaa tttttggttc gcccgcgtgg
     2761 ttttcgaacg cgaccaatgg gaccagcact tggaaaggtg gtcgaagcgg ctggacatca
     2821 ggcttgccat cgttttgcac gcggcgcgac tttgtgtctg gctgacccgc cgcatccttt
     2881 ggctgctgat gcacgccggt catggcatca gttgcttcat gctgcggcag atggaatacg
     2941 atgccgacag ttacgaaacg aagctcgctg gcagtgacgc tttcgaatcc accgccacgc
     3001 gtctgcgcga attgaatgcc gccatgcaac tggcggtgga agaagtccgg caggattggg
     3061 caagccgccg tttgcccgaa aatcttccgc tgctcgtcca gcacaaggcc gcttcgttgc
     3121 ccgccgaact gcaacaaaaa tacgtcggcg ccgcagattc gaaaaaaacc ggatggttcg
     3181 acacgcatcc ttgcgattcc gaccgcgtca aggctgcccg cgcattggcc caaccgggcg
     3241 ttttccgcat tgagcggccc gcgacagagt tgttcgcgga cttcaacgag acgggcaagg
     3301 cagtcacgcg gtttcaatac gagaagcatt ggaaactgga attttcagcc caaaacctcg
     3361 ttgcgtccga agaaatgttg cgtgaaagcg ccgtcaatgc agaggcggac gcggcgatcc
     3421 gtaaatttta tggcgaggtg aacatcaccc tcgcgccttt gctcaacgat gcgcaactgc
     3481 cgccattgac ggaccgtgac accgcgatga ctcaatggcg tgaagcgcgc gaagaaagct
     3541 tacgattacg gcctcaagcg gaaaaagctt ccggcgaatg tctggtccag caggcgcgct
     3601 tggtccagat gatgggagcg tggagcctca gccgggcggg attcaaactc gaagcgaaag
     3661 ccttcggatt ccccgcttcc gccacttcgg tggccgacca ggagattgcc ggtaaatttg
     3721 cggtggaaga agcctcagga acgattcgcc aacaaattca aatgctcaat ccattcgtcg
     3781 ccgcattgcg gactcggatc acgttggcat tgcaattgac gcggccagcc gcaacgaacg
     3841 gagaaccagc atctgaattg gacaaactga taaaagcgct ggctgcgacc ggtaagacgt
     3901 tgccgacctt gcatgaaatc ggcatccggc tgcggccgtt cacggtgctg gcgcaaaatc
     3961 gcggcaacca tcgcaatccg atggaagtca gctcgcacat gaatcgcatc gcggacgaac
     4021 tgcaaaagct cgctgccgaa gcgcaattgc cgctgggcgg acttgcgtat ccatttccgc
     4081 atccccgcgg gcaattgagc gtcattgaat acgcgcgtta cgaaaagccg acccaaaacc
     4141 gctgggaaga agcgtataa
//
LOCUS       DASZMM010000583         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3813106, whole genome shotgun sequence.
ACCESSION   DASZMM010000583 DASZMM010000000
VERSION     DASZMM010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3813106"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..529)
                     /locus_tag="VG754_14010"
     CDS             complement(233..529)
                     /locus_tag="VG754_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWF20381.1"
                     /translation="MIRVVLPFHLRKLAQVENEVQIELNGPATVGGVLDALETHYPIL
                     RGAVRDHVTLKRRPFVRFFACKEDLSLEPPETKLPDEVIQGTEPFLIVGAMAGG"
     gene            complement(526..1692)
                     /locus_tag="VG754_14015"
     CDS             complement(526..1692)
                     /locus_tag="VG754_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HWF20382.1"
                     /translation="MSKIRVLVGTKKGAFILTSDGKRDKWDVSPMHFAGWEMYHLKGS
                     PADPNRIYASQSSGWFGQIIQRSDDGGKTWSPPGSKPEDLKGPEGMPKGESNKFVYEG
                     QVGTHKWYDGTQHPWEFKRIWHLEPSLTDPDTAYAGAEDAALFKTTDGGKTWKELSGL
                     RKPKSNLWQPGAGGMAVHTIILDKKDPKRIFVAISAAGAFRTDDGGITWKPVNKGLKS
                     QFELPDPDADVGHCVHRIAMHPSRPNVLFMQKHWDIMRSDNAGESWTEVSGNLPSDFG
                     FPIDVHAHEPETIYVVPIKSDSEHYPPDGKLRVYRSKSGGNEWEALTKGLPQKDCYVN
                     VYRDAMAVDSLEPCGVYFGTTGGQVYASADSGNSWNPIVRDLPAVYSVEVQTLP"
     gene            complement(1917..2429)
                     /locus_tag="VG754_14020"
     CDS             complement(1917..2429)
                     /locus_tag="VG754_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWF20383.1"
                     /translation="MKRLAFLYLLVIMGAMGGCKPSSTATSPPSAPPSAYPTAPQPKL
                     QTIKLWIGQQEMITEMALTPVQQETGMMFRTNMAENEGMIFPFSQPIRADFWMKNTIL
                     PLSCAYIGPDGTILEIHDMKPQDTNSIVAETSNIQYVLETRQGWFKRNNVGVGTVITT
                     EHGPLHQVFR"
     gene            2524..>4023
                     /locus_tag="VG754_14025"
     CDS             2524..>4023
                     /locus_tag="VG754_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20384.1"
                     /translation="MAILPETKTGEARRNCAENLPWLLGLFLIGLAAKFQLIHKYSSS
                     LPYMDQWSSEVHELFVPWFNHQFSLAALFEAHNEHRIVFTRLSELGLLSANGRWDNRL
                     EITFNAVLHAAAIAGFAWLMISLLGKRVRPWLWTLLVLDLVLPFAWENTLWGFQSQFY
                     FLQIFSVLTIWLLGLSEPLSFRWWCGVACAVLSLFTMASGFFAAVVVMGIVLVDVWKQ
                     RQNWRRHLPTIVVCIGLMAAGWLLKPDVLEDHQYQAHSAGEFLTALGKNLAWPWATKP
                     FYASFNLLPLVLLAWMHFRFASENAGGERMVLGVGFWVILQALAVAYARGVGGIAPAW
                     RYMDVLSFLMIANGLSIWLLLTRYGVRLKFPVLWQAGFALWAVCCLGGLYYLSERAWA
                     KYIPDFALEQQKQLESARAFLATDDVHVLLNRPPVQLIAGNIDEVVWFLRNPMIRQIL
                     PACARDAIPSTDSKLAGQKEGWLSNCAMQMLRGWKFFLGAGLLSLAYGAI"
BASE COUNT          801 a          998 c         1154 g         1070 t
ORIGIN      
        1 agatgcattt tcgaactgca ttgcgggcgc aaggcttgcc cgagccgccc aagacggagt
       61 ggcgtcgtcc gaattccaag tgaattggca ttcttcagtt tcccgtcttc ttcaaaatta
      121 ggaaatcagg aactcaggaa aaggctgccc tgaccgttta cagcttcatt cctgatttca
      181 tgatttccta ataaacctta atgcgcattt ttgtatggcc ctttcaccga attcaccctc
      241 cggccatcgc gccgacaata agaaacggtt ccgtcccttg gatcacttcg tctggcaact
      301 tggtttccgg tggttcgagg gataaatctt ctttgcaggc gaaaaaccgg acgaatggcc
      361 tgcgcttgag tgtcacatgg tcgcggactg ccccgcgcaa aatgggataa tgcgtttcga
      421 gcgcatcaag aactccgccc acggtggccg gaccgttaag ttcaatctgc acctcgttct
      481 ccacttgggc gagcttgcgc aaatgaaatg ggagaacgac gcggatcatg gcagggtttg
      541 aacttcaacc gaatatacgg caggcaagtc tcgcacgatg gggttccagg aattgccgct
      601 gtcagccgat gcatatactt gtccgccagt ggtgccgaaa tagacgccgc agggttcgag
      661 cgaatccacg gccatcgcgt cgcgatagac gttcacataa caatcttttt gcggcaaacc
      721 tttggtgagc gcttcccact cgttgccgcc tgatttgctg cgatacacgc gcaatttgcc
      781 gtccggcgga taatgttcgg aatcgctttt gatggggacg acataaatcg tctccggttc
      841 gtgcgcgtga acgtcaatgg gaaagccgaa atcggaagga agattgccgc tgacttccgt
      901 ccaagattcg cccgcattgt cgctgcgcat gatatcccaa tgtttttgca tgaacaacac
      961 gttcgggcgc gatggatgca tggcaatgcg atgcacgcaa tggccgacgt cggcatccgg
     1021 atcaggaagt tcgaattgag atttgaggcc cttgttcacc ggcttccaag tgatgccgcc
     1081 gtcatcggtg cggaacgctc ccgcagcgga aatggcaacg aagattcgct ttggatcctt
     1141 cttgtcgaga atgatggtgt gcaccgccat gccgcccgcg ccgggttgcc agagattgct
     1201 cttgggtttg cgcagaccgg aaagttcctt ccatgtcttg ccgccgtcgg tcgtcttgaa
     1261 aagcgcggcg tcctccgcgc cggcgtaagc ggtgtccggg tccgtcagcg aaggttcgag
     1321 atgccagatg cgtttgaatt cccaggggtg ttgcgtaccg tcataccact tgtgcgtgcc
     1381 gacctggcct tcataaacga atttattgct ttcgcctttg ggcatgcctt ccggcccttt
     1441 caaatcttcc ggcttgctgc cgggagggct ccaagttttg ccgccgtcat cggaacgttg
     1501 gatgatttgg ccaaaccagc cgctgctttg cgaggcgtag atgcgattcg gatctgcggg
     1561 cgaacccttg agatgataca tctcccagcc ggcgaaatgc atcgggctga cgtcccattt
     1621 gtcgcgcttg ccatcggaag taaggatgaa agcgcccttt tttgtgccca ccaaaactcg
     1681 tattttgctc atagttattg tctcgttaag aattaacagc ttcggtcact ctctgttacg
     1741 acgaacgggt cggcgtcgtg aggacattta ttctgggttt tagtattttg gcaatggaca
     1801 aatgatgtga tacggaattg ttcgccgatt tgtcaggcta attagtgacc ggcgtgttcg
     1861 cgaagcccat tttacccaaa gaattggggg gccggaatct caaccgttgg tcagtgtcat
     1921 cgaaaaactt gatgcagcgg tccgtgttca gtggtgatga ctgtgccgac gccgacgttg
     1981 ttgcgtttga accagccctg gcgggtttcg agcacgtatt ggatattgga agtctcggcg
     2041 acaatggagt tggtgtcctg cggcttcatg tcgtggattt caaggatggt tccgtcaggg
     2101 ccgatatagg cgcaggagag cggaaggatg gtgtttttca tccaaaaatc cgcgcggatg
     2161 ggttgggaga aaggaaaaat cattccttca ttctcggcca tgttcgtgcg gaacatcatt
     2221 cctgtttcct gctgcacggg agtgagcgcc atttcagtga tcatttcctg ctggccgatc
     2281 cagagtttga ttgtctgcag cttcggttgc ggggcggttg gatatgcaga tggaggggcg
     2341 ctcggcggtg aagtggcagt ggaggacggt ttgcaaccgc ccattgcgcc catgatcacc
     2401 agcaaataca aaaacgccaa ccgtttcatg caaacagatt gcggcaagca cccactcgat
     2461 tcaatcctga tgattgggag tttgtgcttt accggacatt gcgctcaggt agcttgaacc
     2521 ggcatggcaa tcttgccgga aaccaaaacg ggcgaggcac ggcggaattg cgcggaaaac
     2581 ctgccgtggt tgttgggtct tttcctgatc ggccttgcgg cgaagtttca gttgatccac
     2641 aagtacagct cttctttgcc gtatatggac caatggtcgt cggaagtcca cgaacttttc
     2701 gttccctggt tcaatcatca attttctttg gctgctctct tcgaggcgca taacgaacac
     2761 cggattgttt tcacacgtct gtccgaactg ggacttctgt cggcaaatgg acgttgggat
     2821 aaccggctgg aaatcacctt taatgccgtc cttcacgcgg cggccattgc cggtttcgca
     2881 tggctgatga tttcgttgct tggaaaacgc gtccgtccgt ggctttggac attgctggtt
     2941 ctggatttgg ttcttccgtt tgcgtgggaa aacacgcttt ggggatttca atcgcagttt
     3001 tatttccttc aaattttttc ggtgctcacg atatggcttc tcggtttgag cgagccatta
     3061 tcgttccgct ggtggtgcgg cgtggcctgt gccgttttgt cattgttcac catggcttca
     3121 ggatttttcg cggcggtggt ggtcatggga attgtgctcg tggatgtttg gaaacagcgt
     3181 cagaactggc ggcggcatct gccgacaatc gttgtctgca tcgggctgat ggcagcaggg
     3241 tggttattga aaccggacgt tctggaagat catcagtacc aagcgcattc tgcgggcgaa
     3301 tttctaaccg ccttgggcaa aaatcttgcc tggccttggg cgacaaagcc attttacgcc
     3361 tcgttcaact tgttaccgtt ggttttgttg gcatggatgc actttcgttt cgccagtgag
     3421 aacgctggcg gagaacggat ggttcttggg gttggatttt gggtcattct gcaagctttg
     3481 gctgtcgctt acgcgcgggg agtgggagga atcgctcctg cctggcggta tatggatgtc
     3541 ttgagtttcc tgatgatcgc caacggcttg agcatctggc tgcttctgac tcggtatggt
     3601 gtgcgattaa agtttccggt tctgtggcaa gcaggctttg cgctatgggc tgtctgttgc
     3661 ctcggcggtt tatattatct gagcgaacgg gcgtgggcca aatacatccc tgattttgcg
     3721 cttgagcagc agaagcaact tgagtctgcc cgcgcttttc tggccacgga tgacgtccac
     3781 gtgctgttga atcgtccacc cgtgcaactc atcgcgggca acattgacga ggtggtctgg
     3841 tttttgcgga acccaatgat ccggcaaatc ctgcccgcat gcgcgcgcga tgcaatacct
     3901 tccactgatt cgaaattagc cgggcagaaa gaaggctggc tgtcgaattg tgcgatgcag
     3961 atgttgagag gttggaaatt ttttttgggc gcgggacttc tcagtcttgc ttacggggca
     4021 att
//
LOCUS       DASZMM010000584         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1092262, whole genome shotgun sequence.
ACCESSION   DASZMM010000584 DASZMM010000000
VERSION     DASZMM010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092262"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VG754_14030"
     CDS             <1..634
                     /locus_tag="VG754_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF20385.1"
                     /translation="RASYSSPIAATVDGRRYAFFFTRQGLVAINPGDGKIEFQFPWAP
                     RIQASVSSATPLVIDDLVFLSASYGAGAVLLRVKNNGVEKIWSAEDVLSNHYATSVEH
                     NGFLFGIDGRTDPGFSPPPSLRCVELKTGKIRWQTDDIGAATITLAGYQLLILTEKGE
                     LIRAAADADAFKPNARAQILPFQVRAYPALADGCLYARSKDKLVCVNLGK"
     gene            932..2185
                     /locus_tag="VG754_14035"
     CDS             932..2185
                     /locus_tag="VG754_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HWF20386.1"
                     /translation="MAESTTIAPAVSASGHQMTASPAASQRLMSVDALRGFDMFWIVG
                     ADEIFLALNKMFKSQPMPAIMNQLDHCEWEGFHFEDFIFPLFVFLMGASVVFSLTKII
                     AQEGRGAAVKRVIRRFVFMFVLALIYSGGFSKNWPDMRLMGVLNRIALCYLFGSLIFV
                     FFKPKAIIGIAASLLLGYWALLAWVPYPDVRPTPGVGIINRTNAPTLEQINLSSTTMI
                     HGSYIQGVNLTDYLDQKYLPGRKYDGTYDPEGFLSTMPAIVTCLLGIFAGLLLKNAEV
                     CPTRKVVYLISFGIGSVLLGYLWGTQFPVIKKIWTSSYVLVAGGYGAILLGVFYWIVD
                     ILKFQAWCQPFVWMGMNSITIYFIHGFIVSFPRLSTRLVGGDIEDFLNNHWTGLGDLL
                     VALVATALTFWLLHFLHRRKIFLRL"
     gene            2675..>3523
                     /locus_tag="VG754_14040"
     CDS             2675..>3523
                     /locus_tag="VG754_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF20387.1"
                     /translation="MFLIIGSTVLATEHHGSDWPHFLGPHANGISDETRLLDSWPTNG
                     PPLVWEKSIGTGYGAPSVIGNQLVFHHRVGDEEIVECLDAVTGKSLWHFNYPSHFEDP
                     YGYNNGPRSTPVLTSNFCYTFGAEGKLVCLKMANGELVWKRDTSFDFNVPNAFFGVGS
                     TPILEGDLLLVIVGGQPNSGMVAFDARTGKTVWESVGKANWEGVPMTGWPGEQIVRWQ
                     SWEKQASYSTPIAATIHGKRQVLCLMRQGLVSLDPKSGAVNFSFWFRSRANDSVNAMC
                     PVVVEDM"
BASE COUNT          755 a          947 c          957 g          864 t
ORIGIN      
        1 ccgtgccagc tactcgtcac ccatcgcggc cacggtggat ggacgccgtt atgcgttctt
       61 tttcacgcgg cagggattgg tcgcgataaa tcccggcgat ggcaagattg aatttcaatt
      121 tccctgggcc ccgcgcatcc aggcatcggt gagttccgca acgccgctgg tgattgacga
      181 tttggttttt ctttccgcca gctacggcgc gggcgcggtc ttgttgcgcg tgaaaaataa
      241 tggggtggaa aaaatttggt cggcggaaga cgtcctgtcc aatcactacg caaccagtgt
      301 ggagcacaat gggtttttgt tcggcattga tggccggacg gatccgggat tcagtccgcc
      361 gccaagtttg cgttgcgtgg aattgaagac cggcaaaatc cgctggcaga ccgatgacat
      421 cggcgcggcc actatcacgc tcgcgggata tcaattgttg attctcacag aaaaaggcga
      481 attgatccgt gccgctgcgg acgccgatgc tttcaagccc aatgcccgcg cgcaaattct
      541 tccattccag gtgcgcgcct atcctgcatt ggcggacggc tgcctctatg cccgcagcaa
      601 agacaaattg gtctgcgtga atttgggaaa atgagaagga aagtgatcga ataacctatg
      661 aagcaagaat attcggttga aaggggagcg cacgcctccg gcgtgctgtg tttggcgtcc
      721 cgccgaacac ttcgtaattc cgatttgcct tatcggccag tgttgaaaga tcagtggttg
      781 aagtcttcgc cgagacggct cagacagcac gccggaggcg tgcgctccga tccaacagct
      841 ttggctttca tccccgactt tcaccttgaa tcctagcttg gaatatttta aaaacattta
      901 acacgcagcg cgcaatgcgc accaaaacca aatggctgaa tcaaccacca tcgctcccgc
      961 agtctccgct tcggggcatc aaatgaccgc cagtccggcc gcttcgcaac ggctcatgtc
     1021 cgtggacgcc ctgcgcggat ttgacatgtt ctggatcgtc ggcgccgacg aaatttttct
     1081 ggcgttgaac aagatgttca aatcccaacc gatgcccgcc atcatgaacc aactcgatca
     1141 ttgcgagtgg gaaggttttc atttcgaaga ttttatcttt cctctgttcg ttttcctcat
     1201 gggtgcgtcg gtggtttttt cactgacaaa gatcatcgcg caggagggcc gcggcgcagc
     1261 ggtgaagcgc gtcattcggc gtttcgtttt catgtttgtt cttgccctga tttattcagg
     1321 tggtttttca aaaaactggc cggacatgcg tttgatgggc gtgctcaacc gcatcgcgtt
     1381 atgttatctc ttcgggagtt tgatttttgt gtttttcaaa cccaaggcca tcattggcat
     1441 cgcggcgagc ctgcttttgg gatactgggc cttgcttgcg tgggtcccct accctgatgt
     1501 gcggcccacg ccgggagttg ggatcatcaa ccggacaaac gccccaacgc tggaacaaat
     1561 caacctgtcc agcaccacca tgattcacgg cagctacatc cagggcgtga acctgaccga
     1621 ttatctcgac caaaaatatt taccgggtcg gaaatatgac ggcacttacg atcctgaggg
     1681 tttcctgagc actatgccgg caatcgtgac ctgtctcttg ggaattttcg ctggcctgct
     1741 gttgaaaaac gccgaagtct gcccgactcg aaaagtggtt tatcttattt cattcggcat
     1801 cggaagtgta ttgctcggtt atctgtgggg gacgcaattc ccggtgatca aaaaaatctg
     1861 gacctcttct tatgtgctgg tggcgggtgg ttatggcgca attttgctgg gcgtttttta
     1921 ttggatcgtg gacatcctga aattccaggc ttggtgccag ccattcgtct ggatgggaat
     1981 gaactccatc accatttatt tcattcacgg cttcatcgtc agctttccga gattatcaac
     2041 gcgtctcgtc ggcggcgaca tcgaagattt tttgaacaat cattggaccg gcttgggcga
     2101 tttgctggtt gctctcgtcg cgacggcgct caccttctgg ctgctgcatt tcctccatcg
     2161 ccggaagatt tttttgcgtt tgtaacaatt gcaaaatatt tccaaacatt ttcgcaactt
     2221 tcctgtctca ccattgttcg cacatgcaac agtaacgaac gaaaggaagg gtttgcgctc
     2281 gcgcgggcaa atcgacgaat gggcgagagc aataaataat gagacacaac cggccttgcc
     2341 cgcgctgcgc gcatattgcg ccgccgggat tgcctgggag ggaaccgaaa tgaacgcgcg
     2401 ccgcagtggg cgcgcaggga atggtgtggt ggccgctggc ggcatccgca aaccggatgc
     2461 aacaagcgcc tccacacctt ttttcccaag tagcgcaggc ttgcagcctg ctgtatcgca
     2521 gagttgcact ctgcggacgc atctacattg cgatcagcgc gaaagaattc cttcacgctt
     2581 ctcaattcgc ctatcatgcc ggctgcaagt cggcgataca gcagagcgga actctgcgct
     2641 acgtgttccc attcgcacac gtcttttgat aattttgttt ctgataattg ggtccacagt
     2701 cctcgcaacg gaacatcacg gctcggattg gccgcatttt ctcgggccgc acgccaatgg
     2761 catttccgat gaaactcgtt tgctcgattc atggccgacg aatggcccgc cgctggtttg
     2821 ggaaaagagc atcggaacag gttacggcgc gccctcggtg atcgggaacc aactcgtatt
     2881 ccatcatcgt gtcggcgacg aagaaatcgt cgaatgcctg gacgccgtta ccggaaaatc
     2941 gctctggcac ttcaattacc caagccattt cgaagaccct tacggctaca acaacgggcc
     3001 gcgttcaaca cccgtgctca cttccaattt ctgctacacc ttcggcgcgg aagggaaatt
     3061 ggtctgcctt aaaatggcca acggcgaact cgtttggaaa cgcgacacca gttttgattt
     3121 caacgtgccc aacgcattct ttggcgttgg cagcacgccc attttggagg gcgacttgtt
     3181 gctggtgata gtcggcggac agccgaattc ggggatggtc gctttcgacg cgcggaccgg
     3241 caaaaccgtt tgggaaagcg tcgggaaggc aaattgggag ggcgtgccaa tgaccggctg
     3301 gccgggcgaa caaatcgtgc gctggcaatc gtgggaaaaa caggccagct attcgacccc
     3361 gattgcggca accatccacg gcaaacgaca ggtgctttgc ctgatgcgac aaggattggt
     3421 ttcgcttgat ccaaaaagcg gcgcggtgaa tttcagcttc tggtttcgct cgcgcgcgaa
     3481 tgattccgtc aacgcgatgt gccccgtcgt cgttgaagac atg
//
LOCUS       DASZMM010000585         8522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2179943, whole genome shotgun sequence.
ACCESSION   DASZMM010000585 DASZMM010000000
VERSION     DASZMM010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179943"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VG754_14045"
     CDS             <1..636
                     /locus_tag="VG754_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20388.1"
                     /translation="RAGFKLEAKAFGFPASATSVADQEIAGKFAVEEASGTIRQQIQM
                     LNPFVAALRTRITLALQLTRPAATNGEPASELDNLIKALAATGKTLPTLHQIGIRLRP
                     FTVLAQNRGNHRNPMEVSSHMNRIADELQKLAAEAQLPLGGLAYPFPHPRGQLSVVEY
                     ARYEKPTQNRWEEAYNDGNAHLERLFSLHYRLLGRLLALAIASEGELDTTK"
     gene            639..1451
                     /locus_tag="VG754_14050"
     CDS             639..1451
                     /locus_tag="VG754_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20389.1"
                     /translation="MKRKFCATVVTLTGAFAFAENAQACEPILPFMKVVGGPAMFMGS
                     WVALASVVVIKSIIFSFSQKKLNFGRAFAFMIAGNVLTTLIGVIVAVMMGSGGLTLLG
                     ALIVWPLCVPPARRVLATSDQKWLKRLSAGSLAFLMTLAFIASCILFALSYFNEDETH
                     LAVHWVLKLGAVYIALIASLVLTAFWEEWVVWKLSCCAVEYTGYVQPVIRANLIVLLC
                     VMLYAAAVMLPQRLKSPHFLAQLYRQTVPLASPTGDTYGRLDSAWYNCPHDS"
     gene            complement(1980..2477)
                     /locus_tag="VG754_14055"
     CDS             complement(1980..2477)
                     /locus_tag="VG754_14055"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HWF20390.1"
                     /translation="MATLPQLIEEDVQRLDGILQELLAESEASTALIIDKGGFLITHR
                     GESKEFDLTTIAALASGAYLANQTIANLIHEENFDSVYQQGEKFSMFVMNVDEYCLLT
                     IIFRAAVSVGAVKYFALRAGPRIADQLLMAHERDPENGLDLSILNIADTAPVFKKKAK
                     GRKTA"
     gene            complement(2488..3111)
                     /locus_tag="VG754_14060"
     CDS             complement(2488..3111)
                     /locus_tag="VG754_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HWF20391.1"
                     /translation="MAIINQATRELQVKIVYYGPAMGGKTTNLVQVHDHVQTAAGNKG
                     KLVSLATSSDRTLFFDFLPIEAMSIKGFKTKFQLYTVPGQVIYNTTRQLVLRGVDGIV
                     FVADSQYEKMAENVESFNNLEENLRALKLNIADIPYVLQYNKQDLPNVAPVEYMEFLL
                     NNRELHVPSFTATAHKCEGVFESLNMITRMLLNKFISQNQNVSQAAA"
     gene            complement(3124..5352)
                     /locus_tag="VG754_14065"
     CDS             complement(3124..5352)
                     /locus_tag="VG754_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20392.1"
                     /translation="MIDSTVPRERPLAPPLSRPAQQPPRAARTDSAPSVPRAPINATG
                     VLNVPLSSVLARLPAELGQRVRQADVGYAEISIPLQRILSQIALGAVKITFGELRQAS
                     PPGTFSAEPDQDRAQVDIPLIEILSRLDPAMLPRRASQKQVVVPADVTGPFGGQNQVN
                     FTIAPLKPAAPAAPAAPAPVAPMAPIPVTPQRAPTPAMVPFNPIPAKPVAPAQPAPKA
                     QPAVVPFQPVQPIKPVQPVQPVQPAKPVQPAQPARPFQPAIAFTQNAPAARPVAPTPP
                     ATVPFQRTAPVPQPQQPAVVPFQRTAPAPQAQQPVAPASPEFVPFQRATPAPQAQQPV
                     APASAPLRVPMPPSNGFKPDTALFKKAAPVPQAAPPQPAYVPEPPAEEPPAIPVVPET
                     NLFTPISPIAPEPEPEPDPIPFNSEPEPVAASAIPAPSIPMPQHSIAGETRFLTLPIA
                     EIAGGWPAAMQQEIMTHQLMTCALALPFGIIELAIKQGRVAIPWRILRGWIKPPVNIP
                     ASPNDAMMLELPLKVVTPLFLAELRAGVPQKKIAVDENIPDLFSNRADSASPVPFVPG
                     AMHAPAAHAPANVSQVVPLNTVSVAPKHDTNYFAKVNGDQHEEAAPPIKRGASPGTAF
                     LARYATPNDIVSKASTLQGVEGALIALPDGLLVSSSIPANMNGDTIAGFLSQIFNRVT
                     QCTKELRMGELNNLNFTVGNVPWKVFKVGAIYFAAFGRPGQPLPTAQLAEIAAELDRK
                     AK"
     gene            complement(5443..5577)
                     /locus_tag="VG754_14070"
     CDS             complement(5443..5577)
                     /locus_tag="VG754_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20393.1"
                     /translation="MARFLPVSTSLNRQMAGVNRVVMRQNASIAQPERVQQVMTALVP
                     "
     gene            5899..6948
                     /locus_tag="VG754_14075"
     CDS             5899..6948
                     /locus_tag="VG754_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF20394.1"
                     /translation="MQPKILLVDDDKDLLEVYREILAQLPSHPEIFTSNTGARALAML
                     ENEPFTIMVCDLNMPKMDGLQVLSIVRRKYPELRTVVLTSLLDEQFRSRAYGLGVDLY
                     WQKPTSTEETKQFLECLESLVGRENGASGFRGVQSKSLVDLIQLECLSHNSTVLKIIN
                     GPLTGRIWIQAGEVIDAATEELTAEPAFHRILSWKAGNFEALPLEPGRARTILNSYQG
                     LLLETAQAQDEALSQGAKANNDSPLTTLSRMAGVSYALVLKPAGSDPKFEARGLESPQ
                     PVVDWSRKTLEQFRALGERLQAGPLQHFDGLGPQGHVGLASIRDLDICVGWEPALSTT
                     ELRESMKKALALWVS"
     gene            6936..7220
                     /locus_tag="VG754_14080"
     CDS             6936..7220
                     /locus_tag="VG754_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20395.1"
                     /translation="MGFLNLFDKPAKLALLPRGSFTVDKEGRVLVSTLPQSFPVEIAE
                     EIATTVLEAFHSAKTARITLNELIVRFANLKMTARELRGGAIIFLAPQTL"
     gene            7263..7658
                     /locus_tag="VG754_14085"
     CDS             7263..7658
                     /locus_tag="VG754_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20396.1"
                     /translation="MNQLIQQLENYTECWKQFISFVNIAHGKKFGQEDENQFLEVKTV
                     IVQQMEMILAVIESGTPPKEEVLQFIGSAPSLRSVAEMNEPALRSLESQWHKLYIGWH
                     SVLGQLKVQQRSLESRSMLGSFFEKKKKD"
     gene            7808..>8522
                     /locus_tag="VG754_14090"
     CDS             7808..>8522
                     /locus_tag="VG754_14090"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWF20397.1"
                     /translation="MPDWISIVILGIIEGITEFLPISSTGHLLLAEHWLPHQTDLFNV
                     VIQTGAVLAVIPLFHQRFHQFIYRWNDPAVRAYLFKIIVAFVITGIGGFILEKKNFKL
                     PEHLTPVAWALLIGGIVFIAVETMLRGKKLSTEVTWTIALAVGLGQLIAAAFPGASRS
                     GTTIMISMILGMARPEATEFSFLVSIPTMLAAGGYKMFKALHHPALGAPHENWPMVLL
                     ATVVAAVVSFIAVKWLLRYV"
BASE COUNT         1804 a         2295 c         2498 g         1925 t
ORIGIN      
        1 cgggcgggat tcaaactcga agcgaaagcc ttcggattcc ccgcttccgc cacttcggtt
       61 gccgatcagg agattgccgg taaatttgca gtggaagaag cctcaggaac gattcgccaa
      121 caaattcaaa tgctcaatcc attcgtcgcc gcattgcgca cccggatcac gttggcgctg
      181 caattgacgc ggccagccgc aacgaacgga gaaccggcat ctgaattgga caacctgata
      241 aaagcgctgg ctgcgaccgg taagacgttg ccgaccttgc atcaaatcgg catccggctg
      301 cggccgttca cggtgctggc gcaaaatcgc ggcaaccatc gcaatccgat ggaggtcagc
      361 tcgcacatga atcgcatcgc ggacgaactg caaaagctcg ctgccgaagc gcaattgccg
      421 ctgggcggac ttgcgtatcc attcccgcat cctcgcgggc aattgagcgt cgttgaatac
      481 gcgcgctacg aaaagccgac ccaaaaccgc tgggaagaag cgtataacga cggcaatgcg
      541 cacctcgaac gcctcttctc gctgcattat cgactgctcg gacggttgct ggctttggca
      601 attgcttcag aaggggagtt ggacacaacg aagtgataat gaagcgaaaa ttttgcgcga
      661 cggtggtcac cttaactggc gcatttgcat ttgccgaaaa cgcgcaagca tgtgagccga
      721 ttctgccttt catgaaggtc gttggcggac ctgcaatgtt catgggttcg tgggtcgccc
      781 ttgcctccgt ggttgtgatc aaatcaataa tattctcctt ctcgcagaag aaattaaatt
      841 ttggtcgagc ctttgcgttc atgattgcgg gcaatgttct tacaacgctg attggtgtca
      901 tcgtcgccgt aatgatgggc agcgggggac ttacgctgct gggagcgctg atcgtgtggc
      961 ctctttgtgt tccgccagcc agacgagtgc ttgcaacctc agatcaaaaa tggttgaaga
     1021 gactttccgc cgggagcttg gccttcctga tgacactggc attcatcgcc agttgcatat
     1081 tgtttgcgct ctcatatttt aacgaagacg aaacgcacct tgcagtccat tgggttctaa
     1141 agttgggagc ggtttatatc gctctcattg ccagcctcgt cttgaccgcg ttttgggaag
     1201 aatgggtggt ttggaaatta tcgtgttgtg ccgtcgaata taccggatac gtgcaaccgg
     1261 tcatccgcgc caatttgatt gtgttattgt gcgtgatgct ttacgccgcg gcagtgatgc
     1321 tgcctcaacg acttaaatcg cctcattttc tggcgcaact ttaccggcaa acagttcccc
     1381 tagcatcccc gactggcgat acctatggga ggcttgacag cgcgtggtat aattgcccgc
     1441 atgatagttg aacctgaaat atgggaagtt ggatggagaa tgaagatgga ttcacctccc
     1501 gattgaaatt ttattaattc ccgtccctcc atgggggcgg gcttgttctt tgacaatcta
     1561 atgttccggg ttccgagttc cggcttccgg agcgcggctc gatgagccgc agtaatacgc
     1621 gacaaccgat tgcgtaagga ttgacctgtg gtgctgcggg tcaccgaccc gcgctccgaa
     1681 ctccaagttg taagtggttg caatcccgca tcgtcccggc tcgtcccgca tcatcccgtc
     1741 aaaaatctca cccctccccc catgcggact gaaacatcga acgcgcaaca tcgaacatcg
     1801 aacaccgaat tgggagaaca ccgggaacat tggatgttcg gcgttcagcg ttggttgttg
     1861 gatgttttgt cctgccgtgt cgcctggtgt tggatgctta gaagatgctt caggcggggt
     1921 aactacaggc cgccttcttt gtttcctttc gctccctttc gagaatggcg agtggcgttt
     1981 tacgcggtct tgcgtccttt ggccttcttc ttgaagacag gggcggtgtc ggcaatgttc
     2041 aggatggaga ggtccaagcc gttctccggg tcgcgttcgt gggccatcaa aagctggtcg
     2101 gcgatgcggg ggccggcgcg caacgcaaaa tatttcacgg cgccaacact cacggcggcg
     2161 cggaagatga tcgtcaggag gcagtattca tccacgttca tgacgaacat gctgaatttt
     2221 tcgccctgct ggtaaacgct gtcgaaattt tcctcatgga tgaggttggc gatggtctgg
     2281 tttgcaagat aggcaccgga cgccagcgcg gcgatggtcg tgaggtcaaa ttccttcgat
     2341 tcaccgcgat gggtgatcag gaaaccgccc ttgtctatga tcagggctgt ggacgcttca
     2401 ctctcggcta gcagttcctg caaaataccg tccagccgct gaacgtcctc ctcgatgagt
     2461 tggggcaacg tggccatggg atttggttca agcggcggcc tggctgacat tctggttctg
     2521 gctgatgaac ttgttcagca acatgcgggt aatcatgttg agcgattcaa aaacgccctc
     2581 gcacttgtgc gcggtggcgg tgaacgatgg gacgtgcagt tcgcggttgt tcaggagaaa
     2641 ttccatgtat tcaaccggcg cgacgttcgg aaggtcctgc ttgttatatt gcaggacgta
     2701 cgggatgtcg gcgatgttga gcttgagcgc gcggaggttt tcttcaaggt tgttgaagct
     2761 ctccacgttc tcggccattt tttcgtattg cgaatcggcg acgaacacga tgccatccac
     2821 gccgcgcaag acgagctggc gcgtggtgtt gtagatgacc tgtccaggca cggtgtaaag
     2881 ctggaacttc gtcttgaaac ccttgatgga catcgcctcg atgggcagga agtcgaaaaa
     2941 gagcgtgcgg tccgaactcg tcgccaagga aacaagcttg cctttgtttc cggcggcggt
     3001 ctggacgtga tcgtggacct ggaccaagtt ggtcgtcttg ccgcccattg ccgggccgta
     3061 atagacgatc ttgacctgca gttcccgggt tgcctggttg attattgcca taagtttaat
     3121 gaattatttt gctttgcgat caagttcagc ggcgatttca gcgagctgcg ccgtcggcag
     3181 cggctggccg ggacggccaa acgccgcgaa ataaatcgcg ccgaccttga atactttcca
     3241 cgggacattg ccaacggtga aattgaggtt gttgagttcg cccatgcgaa gttccttggt
     3301 gcattgggtc acgcggttga aaatctgtga caaaaagccg gcgatggtgt cgccgttcat
     3361 gtttgccggg atgctgctgg aaaccagcaa accatcgggc aacgcaatca acgcgccctc
     3421 cacaccttgc agggtggagg ctttgctgac gatgtcgttg ggcgtggcat agcgcgcaag
     3481 gaacgccgtc cccggcgaag cgccgcgttt gatgggcggc gcggcttctt cgtgttggtc
     3541 gccgttgacc tttgcaaaat aattggtgtc gtgtttcggc gcgacgctca cggtgttcaa
     3601 tggcacaacc tgtgaaacat tggcgggcgc atgggcggct ggcgcgtgca ttgcaccggg
     3661 aacaaacgga acaggcgacg cggaatctgc gcggttggaa aacaggtcgg ggatgttttc
     3721 gtccaccgcg atttttttct gcggcactcc cgccctcaat tccgccagga aaagcggcgt
     3781 gacaactttg agcggcagtt ccagcatcat cgcatcgttc ggcgaagccg ggatgttcac
     3841 gggcggcttg atccagccac gcagaattct ccatgggatc gccaccctgc cctgcttgat
     3901 agcgagttcg atgatgccga agggcaatgc caaggcgcag gtcatcaact gatgggtcat
     3961 gatttcctgt tgcatcgccg cgggccagcc gcctgcgatt tcggcgatgg gcagagtcaa
     4021 aaatcttgtt tcgccggcga ttgaatgctg cggcatcgga attgacggcg cgggtatggc
     4081 ggatgcggca acaggctcag gctccgaatt aaaaggaatc ggatcgggtt ccggctccgg
     4141 ttcgggcgcg atgggagaaa ttggcgtgaa gagattcgtc tcgggaacga ccggtatggc
     4201 cggcggttct tcagcgggcg gctcgggcac atacgccggc tgcggaggag cggcttgcgg
     4261 gacgggagca gccttcttga aaagcgcggt atctggcttg aatccgttgc tgggaggcat
     4321 cgggacgcgc aaaggagcgg atgctggcgc gaccggctgt tgcgcctgcg gcgcgggagt
     4381 tgcgcgctga aaaggaacga attccggtga cgcgggagca actggctgtt gcgcctgcgg
     4441 tgccggagca gtgcgctgaa aaggaacgac tgcgggctgt tgcggctgcg gtaccggggc
     4501 agtgcgctga aatggaacag tcgcgggcgg cgtcggagca accgggcgcg cggcgggcgc
     4561 attttgggtg aatgcaatgg ccggttggaa cggacgcgca ggctgggctg gttgcactgg
     4621 ctttgccggt tgcacaggtt gcacgggttg caccggcttt attggttgca ccggctggaa
     4681 tggaacgaca gcaggttgag ccttgggcgc gggctgcgcc ggtgcgacag gcttcgcagg
     4741 gatggggttg aacggcacca ttgcaggcgt tggggcgcgt tgcggagtaa caggaatcgg
     4801 cgccatggga gcaaccggag caggcgcggc aggtgctgcc ggcgcggcgg gcttcagcgg
     4861 agcaatcgtg aaattgacct ggttctggcc gccaaaagga ccggtgacgt ctgcaggaac
     4921 cacaacttgt ttttgcgaag cgcgccgggg aagcatcgcg ggatcaagac gggaaaggat
     4981 ttcaatcagc ggaatgtcaa cctgtgcgcg atcctgatca ggttcggctg agaatgtgcc
     5041 gggcggcgaa gcctgacgta attcaccaaa agtaattttc accgcgccca gcgcaatttg
     5101 cgaaagaatc ctttgcaaag gaatggagat ttcggcgtaa ccaacgtctg cctggcggac
     5161 gcgctgcccg agttcggcgg gaagacgggc aagaacggaa cttaacggca cgttcaaaac
     5221 gcctgtggcg ttgatcggcg cgcgtgggac ggacggagcg gaatcggtgc gggccgcacg
     5281 aggcggctgc tgggcgggac gggaaagagg aggcgcgagg ggacgttctc gaggaacggt
     5341 cgagtcaatc acatcggttc catgcctgct gaaaagtttt ctgaacgcgc tgaacattgg
     5401 atgtattttt gacagtcctg tcatgtttcg cccaaacaac catcacggca caagcgctgt
     5461 cattacttgc tggacccgtt ctggttgcgc gatgctggca ttttgccgca tcaccacccg
     5521 gttcactccg gccatttgcc ggttgagcga ggttgaaaca ggcaggaaac gtgccacggt
     5581 ttgagcaaaa aggtctttcc ctcaaacccg ctgaagacgc gcattggaga gcattctgat
     5641 tctgcaagtt gccgccgatt cttgccgctt gtgtcctatg acgagacgac gttgtccagt
     5701 ttagacaatg aaaatgtgca ccaatctgct cccgtccgcc gcagagattt gcccaagtcc
     5761 aaacgaactg aatgaaagtc aaaaaaagat gccgtccctc aaatccggaa tgcgatttgc
     5821 tttgattgat gatttgtgac cggggactga ccgtccgccg ggcgaagcaa tcaagaattt
     5881 gagtgacaag caagtcttat gcaaccgaaa atcttattgg tggatgacga caaagatctc
     5941 ctggaagttt accgggaaat ccttgcgcaa ctgccgagtc atcccgagat tttcacttcc
     6001 aatacagggg cgcgcgcgct cgcgatgctc gagaacgagc ctttcacgat catggtctgc
     6061 gacttgaaca tgccgaagat ggacggcttg caggtgctct ccatcgtgcg ccggaaatat
     6121 cccgaattgc gcacggtggt gttgacctcc ctgctcgacg agcaattccg ctcgcgcgct
     6181 tacggtttgg gagttgattt gtattggcag aagccgacct ccaccgaaga aacaaaacag
     6241 tttttggaat gcctcgaatc tctcgtcgga cgcgaaaacg gcgcgagcgg tttccgcggc
     6301 gtgcaaagca aaagcctcgt ggacctcatc caactggaat gcctttcgca caattcaacc
     6361 gttctcaaga tcatcaacgg cccgctcact ggccgcatct ggatccaggc cggcgaggtg
     6421 atcgatgccg cgaccgagga actcaccgcc gaacccgcgt tccatcgcat tctttcctgg
     6481 aaagccggca attttgaagc tcttcccctt gaacccggtc gtgcccggac gattttaaat
     6541 tcgtatcaag gcttgttgct ggaaaccgcc caggcgcagg acgaagcgtt gagccagggc
     6601 gcaaaagcca acaacgattc tccgctcacc acgttgtccc gcatggcagg cgtctcgtac
     6661 gcgctggtct tgaaaccggc gggctccgac cccaaattcg aagcgcgtgg cctcgaaagt
     6721 ccccagccgg tcgtggactg gagccgcaag acgctggaac aattccgcgc gctcggcgaa
     6781 cgtctgcaag ccgggccttt gcagcatttc gacggactcg gcccgcaagg ccacgtcggt
     6841 cttgcgtcaa tccgggattt ggatatttgt gtcggttggg aacctgctct ctcgaccact
     6901 gaactccgcg aatccatgaa aaaagctctc gcactatggg tttcctaaat ctattcgata
     6961 aaccagccaa gctcgccctg ctgccccgcg gcagcttcac cgtggacaag gaaggccgtg
     7021 tcctcgtctc gacgcttccg caatcgttcc cggttgaaat cgccgaagaa attgcgacca
     7081 ctgtcctgga agcatttcat tccgccaaaa ccgcgcgcat caccctgaac gaactcatcg
     7141 tgcgttttgc gaatttgaag atgaccgccc gcgagttgcg cggcggcgcc ataattttcc
     7201 tggccccgca aaccctctga ccagccaact caaatccacc aatgcctatg caaaacaaac
     7261 aaatgaacca gctcattcag cagttggaaa actacaccga gtgctggaag caattcatca
     7321 gcttcgtcaa catcgcccac ggcaagaagt tcggccagga ggacgaaaac caattcctcg
     7381 aggtcaagac cgtcatcgtc cagcagatgg agatgatcct tgcggtgatt gaatccggca
     7441 cgccgccaaa ggaagaagtg ctccaattca tcggcagtgc gccctcgttg cgttccgtcg
     7501 ccgaaatgaa cgagcccgcg ctgcgcagcc tggagagcca atggcacaag ctgtacatcg
     7561 gctggcactc cgttctcggc cagctcaagg tgcagcagcg cagcctggaa tcccgctcga
     7621 tgctcggcag ctttttcgag aaaaagaaaa aagattaagg cacgaatttc accgattatc
     7681 acgaattgga cttgtatttc acagttcgtg aagaattcgt gcaattcgtg tctcttcgtt
     7741 ttttgttcat cccttgttaa aagatttcct gcttgtcatc agcttcgatt tcgcttctaa
     7801 ccatcggatg cctgactgga ttagcattgt tattttagga atcattgagg gaatcacgga
     7861 atttctcccg atttcctcca ccggccattt gttgctcgcc gaacattggc tcccgcatca
     7921 gaccgatttg ttcaatgtcg tcatccagac cggcgcggtg ctcgcggtca ttcccctctt
     7981 ccatcaacgt tttcatcaat tcatttatcg gtggaacgac ccggcggtgc gcgcttatct
     8041 gttcaaaatc attgtggcat tcgtcatcac cggcattggc ggattcatcc tcgaaaagaa
     8101 aaatttcaag ttgccggagc atctgacgcc ggtcgcgtgg gcgctgctca tcggcggaat
     8161 tgttttcatc gccgttgaaa cgatgctgcg cggaaaaaaa ttaagcacgg aagtcacgtg
     8221 gaccattgcg ttggcggttg gtctcggtca attgatcgcc gccgcgtttc ccggcgcatc
     8281 gcgcagcggc acgaccatca tgatctcgat gatcctgggc atggcgcgtc ccgaagcgac
     8341 ggaattttca ttcctcgtca gcattcccac catgctcgct gccggcggtt acaaaatgtt
     8401 caaggccttg catcatcccg ctctcggcgc gccgcatgaa aattggccca tggtgctgct
     8461 cgctacagtc gtcgcggcgg tggtttcgtt catcgcggtc aaatggctgc tgcggtacgt
     8521 gc
//
LOCUS       DASZMM010000586         6180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4932929, whole genome shotgun sequence.
ACCESSION   DASZMM010000586 DASZMM010000000
VERSION     DASZMM010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4932929"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VG754_14095"
     CDS             complement(<1..819)
                     /locus_tag="VG754_14095"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF20398.1"
                     /translation="MKMPSQNSQRILVRGVNWLGDAVMSTPALQRLREARPGAHITLL
                     THAKLADLWQNHPSVDAIETFSEDESAWSIGRRLREKKFDVGLVLPNSHRSALELWLA
                     KIPERIGYARGLRNIFLTRSVALRSGAVTMHKRSTPEIEDLIRENAPQNPPPTGAHHI
                     HQYLHLTAALGAKPEPLPPKLAIAEQAVVDFIAKFKLAANPSETPLWFGLNAGAEYGP
                     AKRWPEERFIDAAIQIHRRTGCHWLIFGLKSDSGMAARIADQVSRGTGGQTAPES"
     gene            complement(823..1869)
                     /locus_tag="VG754_14100"
     CDS             complement(823..1869)
                     /locus_tag="VG754_14100"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20399.1"
                     /translation="MLMRLLQALPLNGVARVGRFFGGLVYYCDARHRRVAIRNLTLCF
                     GAEKSPAEIRAIARENFKRIGENFSCPVKMAIMGRVTFDERIELAGTEKLTARDVPPS
                     TSVIMAVGHFGNFELLAHLKYGMPTHEFATTYRAVRNPAVNKILGQLRASTGCLFFER
                     RSEGAALRNALRTNNLVLGLLSDQHSGDNGLRLPFLGHDCNTSKAPAIFALRFNAPLY
                     TAICYRTQLAHWRVEVGEEIPTHENGQPRSLAAIMLDVNRAFEAAVRRDPANWFWVHN
                     RWKKSKLIAVAEQKDQSLSSSGGEEMECQAIPINSKLTDNSSSSPPLGERVGVRGRVV
                     STPGEANDLEDLQS"
     gene            complement(1903..3075)
                     /gene="lpxK"
                     /locus_tag="VG754_14105"
     CDS             complement(1903..3075)
                     /gene="lpxK"
                     /locus_tag="VG754_14105"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWF20400.1"
                     /translation="MRERFRSWAENIETFYTEVVLENRPGYKAALLRACLYIFSRLFL
                     VGAKFRRVLYDARIIRDSTLGVQVIAVGNLTYGGTGKTPVVEKFARELQDQGRTVAIL
                     SRGYRSKPPPMRTRLLNKLLFREDSTPPRVVSDGKSLLLDSETAGDEPFMLASNLKNV
                     VVLVDKDRVKAGRYAIEKFGCDTLLLDDGFQYWRLQGRRKDIVLIDRQQPFGNEHLIP
                     RGSLREPPSHLARAHYIFITKSDGKTEELRQRIAQHNSTAGLIECIHHPLYFEDVFTG
                     QRHGLDLVAGKKVASLSGIAQPESFEQSLGTLGAALVYSKRFADHHRFTQQEILNTIN
                     RGKKRQAEIIITTQKDAVRFPKLDRRDLPIYFMRVEIKILSGAKDFQDCVSKICFR"
     gene            3211..3498
                     /locus_tag="VG754_14110"
     CDS             3211..3498
                     /locus_tag="VG754_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20401.1"
                     /translation="MAYGTHKLSPTDVPFFFSGFIGVYTVKLVHAHDLQHLIEVNVLW
                     LVWLVLLYWRAKARAGKILGMQVGVYSGFGIASYFVGLLMACVYYLVYANT"
     gene            3520..4797
                     /locus_tag="VG754_14115"
     CDS             3520..4797
                     /locus_tag="VG754_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20402.1"
                     /translation="MNKTAKPIPPPDPEEEEALREVAEEQEEKYGGHDRWVVSLMFLV
                     AAIFAAYEIHRKQQIGLFFLAMSVLSGVFAVINWRSWLKKSPVQRMAERSTNPFASPE
                     IKHCSPPYSESEFTELRNAFGRNIALAVMAFLFGFLGICTAAMPQQPLALRSIGLSVV
                     IAAFYLGRRPAQRFWRAGKTSLACPKCHDLLASQWKQVMQTGRCSKCGAQILTDAAVE
                     DSPADLPTRRRRRTKATGDFQFDPKRIMDVLVLLSGPAFCIFFFGWNKFELGVLVLIG
                     IMLLAMGTIFCFMPDKLLDDESDPHPRRDLKKTEPSSRSSAPPVPGPLPLLDFTVLLA
                     FPIVVVAGLSCLIWAHYVLNKWELALVALALPGAIVLVSYFVARHESYHVKKTGRPAT
                     ERFVWTLIGFWAMAVFVFVVCVVSWIEKFGLKW"
     gene            4965..5576
                     /locus_tag="VG754_14120"
     CDS             4965..5576
                     /locus_tag="VG754_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid hydroxylase"
                     /protein_id="HWF20403.1"
                     /translation="MHNFVFWMTIGFLAAVVYSSFFEWILHRYIMHRPFGKFDYAFRA
                     HAQVHHQTFKADHTYHLIHEKDKHTIPMAWWNGPVLIAAGMAPFWIASWIFGTWGIAA
                     GGLVACASYYGAYEYMHWCMHLPKKRNIERSGIYFRLNGHHLLHHRYMHKNYNVVLPL
                     ADLLLGTLLLRSKVNFKQATGPAVPNVQPRIRKPRLPKLAVNQ"
     gene            complement(5611..6012)
                     /locus_tag="VG754_14125"
     CDS             complement(5611..6012)
                     /locus_tag="VG754_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20404.1"
                     /translation="MRTHPFDLKNELNGKVFEHIKDLSAHSDIVEQLLTAVKPLGDVQ
                     VFTPDGPESYRYYTVSTKGIIFGFAIGMDTIAFRVNLTFKERALASGASPCPDCGPDW
                     VAFKAFRSDWPRPDWEFWARKAYVDVRETTP"
BASE COUNT         1318 a         1655 c         1702 g         1505 t
ORIGIN      
        1 tgattcgggc gcggtttgtc cgccggtgcc acgcgacact tggtctgcga tgcgtgcggc
       61 catgccagaa tctgatttaa gcccgaagat gagccagtgg catccggtcc ggcggtgaat
      121 ttgaatcgcg gcgtcaatga atcgctcttc cggccagcgc ttggcaggac catattccgc
      181 gccggcattc aagccgaacc aaagcggggt ttcggacgga ttcgcagcga gtttgaattt
      241 cgcgatgaaa tccacgaccg cctgttcggc gatggccagt ttgggcggca gcggttcggg
      301 tttcgcgccg agcgcggcgg tgaggtgaag gtattgatgg atatgatgcg ctccggtcgg
      361 cggcggattt tgcggagcat tttcgcggat gagatcttca atttccggcg tggaacgttt
      421 gtgcatcgtc accgcgccgg atcggagagc gacggagcgg gtaagaaaaa tatttctcaa
      481 tccccgcgca taaccgatgc gttcgggaat ttttgccagc cagagttcca gcgcggaacg
      541 atgcgaattt ggaagaacca atcccacgtc gaattttttc tcgcgcaacc gtcggccgat
      601 gctccaggcg ctttcgtctt cggaaaaagt ttcaatcgca tcaaccgacg gatgattttg
      661 ccagaggtcg gcaagcttgg cgtgggtcag caacgtgata tgagcgccgg gacgcgcctc
      721 gcgcaggcgt tgcaacgcgg gcgtgctcat gacggcatcg ccgagccaat ttacgccgcg
      781 gacaaggatg cgttgggaat tttgcgatgg catcttcatc gcttagcttt gtagatcttc
      841 caaatcgttt gcttcgccgg gcgttgaaac aactcgtccc ctcaccccaa ccctctcccc
      901 cagtgggggc gaggaagaag aattgtcagt aagttttgaa tttatcggta tggcctgaca
      961 ttccatttcc tctcccccac tggaggaaag ggattggtct ttctgttctg caacagcaat
     1021 aagtttcgat tttttccaac gattatggac ccagaaccag ttggcggggt cgcggcggac
     1081 ggcggcttcg aaggcgcggt tcacgtcgag catgatggcg gcaagcgaac gcggttggcc
     1141 gttttcatgt gtgggaattt cttcgccgac ctcaacccgc caatgggcaa gctgtgtccg
     1201 ataacaaatt gccgtatata aaggggcatt gaaacgcagc gcaaaaatcg cgggcgcctt
     1261 gctcgtgttg cagtcatggc cgagaaatgg cagacgcaat ccattgtcgc cggaatgctg
     1321 gtcggagagc aggccgagca cgagattatt cgtgcgcaat gcattgcgaa gggcagcccc
     1381 ttcactgcgc cgctcaaaga aaagacagcc ggtgctggcg cggagttggc cgagaatttt
     1441 gttcacggcc gggtttcgca cggcgcgata ggtggtggca aactcgtgcg tcggcatccc
     1501 atacttcaaa tgcgcgagca attcgaaatt gccgaaatgc ccgacggcca tgatgacact
     1561 ggtggagggt ggcacgtcgc gcgcggttaa tttttctgtc ccggccagtt ctatccgttc
     1621 atcgaatgtc acgcggccca tgatggccat tttgaccggg caactgaaat tctcgccgat
     1681 acgcttgaaa ttctcgcgcg cgatggcgcg gatttccgct ggtgattttt ccgcgccgaa
     1741 acacaacgtc aaattgcgga tcgccacgcg ccggtgccgc gcatcgcaat aataaaccag
     1801 cccgccaaaa aatcggccga cgcgcgcgac gccgttgagc ggcagcgcct ggaggaggcg
     1861 catgagcaca tatataaaat ggtaaactgc ccagttcatc agtcaacgga agcagatctt
     1921 gctcacgcaa tcctgaaaat ctttcgcgcc gctcagtatt ttaatttcca cgcgcatgaa
     1981 ataaatcggc aaatcgcgcc ggtcgagttt tgggaaacgc acagcgtcct tctgcgtggt
     2041 gatgatgatc tcagcctggc gttttttgcc gcgattgatg gtgttgagga tttcctgctg
     2101 cgtgaaacga tgatggtccg cgaaacgttt cgaataaacc aacgcggcgc ccagggtgcc
     2161 gaggctttgc tcgaaacttt ccggctgcgc aattccgctc agcgacgcga ctttcttgcc
     2221 cgcgaccagg tcaagcccat gccgctgccc cgtgaacacg tcctcaaaat aaagcggatg
     2281 atggatgcat tcgatgaggc cggccgtgga attgtgttgc gcgatgcgct ggcgcagttc
     2341 ttccgttttg ccatcactct tcgtgatgaa aatgtaatgg gcgcgggcaa gatgcgacgg
     2401 cggttcgcgc aagctgccgc gcgggatgag atgctcgttg ccgaacggtt gctgacggtc
     2461 gatcagcacg atgtctttcc ggcgtccctg caatctccaa tattgaaagc cgtcgtcgag
     2521 cagcaacgtg tcgcagccga atttctcgat cgcgtaacgt cccgccttga cgcggtcctt
     2581 gtccacgagc acgacgacat tcttcagatt cgacgcgagc atgaacggct cgtcaccggc
     2641 ggtttcggaa tcgagcagga gcgacttgcc gtccgacaca acgcgcggcg gcgtgctgtc
     2701 ttcgcggaac aataatttgt tgagcaggcg cgtgcgcatc ggaggcggct tggaacgata
     2761 cccacgcgaa agaatcgcga ccgtgcggcc ctggtcctga agttcgcgcg cgaatttttc
     2821 aaccaccggc gttttgcccg tgccgccgta ggtgagattg ccgacagcga tgacctgcac
     2881 gccaagggtt gaatcgcgga tgatgcgcgc gtcgtaaagc acgcggcgga attttgcccc
     2941 cacgaggaag agacgtgaaa aaatgtacaa acacgcgcgg aggagcgcgg ctttgtatcc
     3001 cggccggttc tccagaacga cttcagtata aaacgtctct atgttttccg cccaggagcg
     3061 gaagcgttca cgcatggcaa tatcagtctg ccaacaggcg gggcgaaggg caaggcgaac
     3121 ggtgcatttt gcaaaactcc gcgttgacgc ccttgatttt gagcagcaga atgcatctga
     3181 gactttccac gcaatttaat ccgccgatat atggcatatg gaactcacaa attaagcccg
     3241 acggacgttc cgtttttttt ctcggggttc atcggcgtat atacggtcaa gcttgttcat
     3301 gcgcacgatc tccagcattt gattgaggtc aatgtccttt ggcttgtgtg gcttgtcttg
     3361 ctttattggc gcgccaaggc tcgcgccgga aaaatcttgg gcatgcaagt cggcgtctat
     3421 tctggttttg gcatcgcttc ctacttcgtc gggctcctga tggcctgcgt ttattatttg
     3481 gtttatgcga acacctaaat atttggcatc caccttcgca tgaacaagac cgccaaaccc
     3541 atcccgccgc cggatccaga agaggaagag gcgttgcggg aggtcgccga ggagcaggaa
     3601 gaaaaatatg gcggacacga ccgctgggtt gtttcgctga tgtttctcgt tgcagccatt
     3661 ttcgcagcgt atgaaatcca cagaaaacag cagatcgggt tattctttct tgcgatgtcg
     3721 gttttgtcgg gagtttttgc ggtaatcaat tggcgctcgt ggcttaagaa atcgcctgtg
     3781 caacggatgg cggagcgttc gaccaatcct ttcgccagtc ctgagatcaa acattgttcc
     3841 ccgccttaca gtgagtctga atttacggag cttagaaatg cattcggccg caatattgcg
     3901 ctggctgtaa tggcgttcct gttcggcttt ctcggcattt gcaccgcagc catgccgcag
     3961 caacccctgg ccttgcgctc tatcggccta agcgtggtaa tcgccgcgtt ttatcttgga
     4021 cgccgccccg cgcaacgttt ttggcgcgcc ggaaaaactt ccctggcgtg cccgaaatgc
     4081 catgatcttt tggcttcaca atggaaacaa gtgatgcaga ccgggcgttg cagcaaatgc
     4141 ggcgcgcaaa tattgaccga tgccgcagtt gaagattctc ccgcggatct gccgaccaga
     4201 aggcgcagaa ggacaaaagc gacgggagat tttcaattcg atccaaaacg catcatggac
     4261 gtgctcgttc tcctgtccgg tcccgcattt tgtatttttt tcttcggctg gaataaattt
     4321 gaactgggcg tactcgtgct cattggcatc atgcttttgg caatgggcac gattttttgt
     4381 ttcatgcccg ataaattgct cgacgacgag tcagatccgc atcctcgacg tgatttaaag
     4441 aagactgagc cttcttcgag atcgagcgcg cctccggttc ccggtccgct gccattgctg
     4501 gatttcaccg tgttgctcgc gtttcctatc gtggtcgtcg ccggtctatc ctgtctgatt
     4561 tgggcacact acgtgctgaa caaatgggaa ctggcgcttg tcgccttggc gttgccgggt
     4621 gcgatagttc tcgtgtctta ttttgtggca cggcatgaat cgtatcacgt caaaaaaaca
     4681 ggcaggcccg cgacggaacg gttcgtttgg acgctcatcg gattttgggc aatggccgtt
     4741 ttcgtctttg tagtctgtgt cgtgagttgg atcgaaaaat ttggcctgaa gtggtgaaag
     4801 aaacatcgcc gtttcatgcg tcatccaatt cctgacaaaa ttgcagttga ctcattggta
     4861 aatcgcaacg aaggtgtccg ctgtcaagcc gtgtgatgat tcgctttttc cgggtggaat
     4921 cttctggtcc ttctcgcggc ttgccagttt tttagaacct aattatgcac aattttgttt
     4981 tttggatgac tatcggcttt ttggccgcgg tcgtttactc ctcatttttt gaatggattt
     5041 tgcaccggta tatcatgcac cggccattcg gtaaattcga ttacgccttc cgcgcccacg
     5101 ctcaggtgca tcatcaaacc ttcaaggccg accacaccta tcatctcatc cacgagaagg
     5161 acaagcacac cattcccatg gcctggtgga atggcccggt gctcatcgcg gcgggaatgg
     5221 cgccgttttg gatcgcgtca tggattttcg gcacgtgggg catcgcggcg ggcggattgg
     5281 tcgcctgcgc cagttattat ggcgcttatg aatacatgca ttggtgcatg catttgccca
     5341 agaagcggaa catcgaacgc tccgggattt attttcgcct gaacggacat catctcctcc
     5401 atcaccgtta catgcacaag aattacaatg tcgtgcttcc gctcgccgac ctcctgctgg
     5461 gcacgctgct cctgcgttcc aaggtgaatt tcaaacaagc caccggcccg gctgttccga
     5521 acgttcagcc ccgcatccgc aaaccgcgcc tgcccaagct cgcggtgaat cagtagaatg
     5581 ttgggcaggt gccctggaac gcagttgttt ttacggtgtg gtttctctca catccacata
     5641 ggcttttcgc gcccaaaact cccaatcagg tcgcggccag tctgaacgaa atgccttaaa
     5701 ggccacccaa tctggcccgc aatccggaca gggtgaggca ccgctggcaa gagcacgttc
     5761 cttgaaggtt agatttaccc ggaacgcaat cgtgtccatg ccgatggcaa atccaaaaat
     5821 aatccctttg gtagaaactg tgtaatatct ataactctcc ggcccgtccg gtgtgaaaac
     5881 ttggacatcg cccaacggtt tcactgctgt gagcaattgc tcaacaatat ctgaatgcgc
     5941 agacaaatcc ttgatgtgct caaaaacctt tccattgagt tcgttcttga ggtcaaaagg
     6001 gtgagtccgc attgcaattc attttgatga tgcactctca tttgtcaaat caatggaatg
     6061 ctcattaaat caaattagaa gctttgaaat tccgtttgcc tttccgcaaa ttttcgcaac
     6121 actgcgcgca tgagtaaaac gacatctcgc aaatttgccg cgtacccgcg cctcaacccg
//
LOCUS       DASZMM010000587         4621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4119422, whole genome shotgun sequence.
ACCESSION   DASZMM010000587 DASZMM010000000
VERSION     DASZMM010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4119422"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VG754_14130"
     CDS             <1..1543
                     /locus_tag="VG754_14130"
                     /inference="COORDINATES: protein motif:HMM:NF037833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWF20405.1"
                     /translation="PTNRFTIGWRNNSTTNAFLPSLQFHGNIDEVSIYNYALSSNQVQ
                     NHYLSAGIIPRLTTVPTNTSVSEDTTATFKAGAIGSLPLSLQWYTSDSSNPQSALSGQ
                     TNGTLTLNNVTAGMNGNYYQFVASNSYGSITSPAVQLTVISGPPILELDLQSSISNYI
                     GFPLVLSATFGGTAPISYRWYYNNNPLSDGGRISGSQSNVLTVADSQPGDAGTYQLVA
                     TNVQGSTPSTAAAVAIVPVLGFNGSGFGWSQQGTSVGSLYAGNNQLQLTFNTGNEDNS
                     SFFDYPVYIGGFEASFLYQVVNPPGTPADGLCFVVQNDPRGAAALGGGGGALGVGGIT
                     PSAELEFNIYPNNPLGGVGIALATNGAIGNVIHTLPVNLISGDVIAVNILSLNGTVYL
                     TLTDTNANATFSASAALNVPAVVGGNAAYVGFTGSDGGAEANQVINNFQFISLVPLSA
                     TPSNGNLVLTWPLGVGGYVLQQRSSLNSGSWANVPNAVTTVGGMNQVTVTAGAAQQFF
                     QLVLP"
     gene            1811..2110
                     /locus_tag="VG754_14135"
     CDS             1811..2110
                     /locus_tag="VG754_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20406.1"
                     /translation="MLPFSATVTNGNITFSWQTMPGKWELLERQPAASVEWKPIPPSQ
                     YQTNGFIVSTILPLPKQKALYCVRRTLGFRPPKLPNLPPVPPLPTNLPPRFKAHP"
     gene            2217..3176
                     /locus_tag="VG754_14140"
     CDS             2217..3176
                     /locus_tag="VG754_14140"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HWF20407.1"
                     /translation="MDRFRRSWDLFKSSLFVMMRHKTLLLFPILTAVLTAFLIILFLV
                     PVAFRPTGYSYTSAQHWKAVSKGIVNSDSPSTHQDSPESGSSNSRLNTLKKVNPIAAV
                     YFAAMYFATMLLATFLNVAFYREILNALSGQPVSIAEGLGFAARKWKIILMWTLFAGL
                     VGFIIKTMEERFGWFGEIVMRLIGTVWSIACVFVIPVIITEEQTSNPFVVLKKSAETL
                     KNTWGESLIGYAGVSAGSSVLMILSLFWLAAGLGTAMYLHSVLLGVLAVASWLFALVV
                     ISYLMSVASQIFRCALFLYASQGTLPHPYNEEMMALAWKTKKS"
     gene            3305..3562
                     /locus_tag="VG754_14145"
     CDS             3305..3562
                     /locus_tag="VG754_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20408.1"
                     /translation="MSEAGGGSMDSTLAEISSAGLESPIPSKRLEHDAQMLGFLDNVF
                     PPSDIGSQQQMQIMGFNFNQHQLISTAFQEKFIHIISNGCE"
     gene            complement(3605..3967)
                     /gene="queD"
                     /locus_tag="VG754_14150"
     CDS             complement(3605..3967)
                     /gene="queD"
                     /locus_tag="VG754_14150"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HWF20409.1"
                     /translation="MKMDLRKTFQFEAAHLLPHLPKSHKCRRLHGHSFKAEIVVSGEC
                     DPKLGWVMDYADITEAFKPIWEELDHHYLNEIRGLENPTSENVAAWIWRKLKPKLPQL
                     TEVVVAETCTARCVYRGD"
     gene            complement(3964..>4621)
                     /locus_tag="VG754_14155"
     CDS             complement(3964..>4621)
                     /locus_tag="VG754_14155"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HWF20410.1"
                     /translation="YGHRVSFGYITKEAFTWANPKKIAARAAADVVAWNQLGCLSPHV
                     IYVENGGEIGAEQFAELLARELERSEAAEPRGELPAESVAIIASKRAFYEIRAAHAPD
                     STRHWCSENSTAWTVIYEADPRFQLSCLNRFIYVKPASNLTDALQNADSVHGKVSTVG
                     LVATEEESGKIAAQLARWGVTRICPLGQMQNPPLLWRHDGRPPLADLVTWTDWEKENL
                     "
BASE COUNT          993 a         1343 c         1187 g         1098 t
ORIGIN      
        1 tcccacaaat cgtttcacca tcggctggcg taataattcc accaccaatg cattccttcc
       61 gagcttgcaa ttccatggca acattgacga agtctcaatc tacaactatg cgttgagctc
      121 gaatcaggtg caaaaccatt atctctcggc aggcataatt ccccggctaa cgaccgtgcc
      181 aaccaacacg tcagtttccg aagacaccac tgccaccttt aaggcgggag ccattggcag
      241 cctcccactg agcctgcaat ggtacacttc ggatagttcg aatccgcaat cggcactgtc
      301 gggacagacg aacggcacgc ttaccctgaa taatgtgacc gcaggcatga acggcaatta
      361 ttaccagttc gtggcgtcca acagttacgg cagcatcacc agcccggctg tgcagttgac
      421 cgtaatcagc ggaccgccaa tcctcgaatt ggacttgcag agttcgattt cgaactatat
      481 cggcttccct ctggtcctca gcgcgacgtt tggcggcaca gcgccgatca gttacagatg
      541 gtactacaat aacaatccgc tctcggatgg cgggcgcatc tccggttcgc aaagcaatgt
      601 gttgaccgtt gccgattcgc aaccgggcga tgcgggcact tatcaactcg tcgccacgaa
      661 cgttcaaggt tcgacaccga gcactgcggc agcggtagcc attgtccccg tgctcggctt
      721 caacggctcg ggcttcggct ggagccagca aggcacttct gtcggtagtc tgtatgcagg
      781 aaacaaccaa ctccagttga cctttaacac tggcaatgaa gacaatagca gcttcttcga
      841 ttacccggtt tatatcggtg ggttcgaggc aagcttcctc tatcaggtcg tcaatccgcc
      901 aggcactcct gctgacggtt tgtgttttgt tgtccagaac gatccgcgcg gagcggccgc
      961 tttgggcggc ggcggcggcg ccttgggcgt gggcggcatc actcccagcg cggaattgga
     1021 attcaacatc tatccgaata atcctctggg tggtgtcgga attgccttgg ccaccaatgg
     1081 tgcgattggc aatgtcatcc acacactccc ggtgaacctc atcagcggcg atgtcattgc
     1141 cgtgaacatc ctctcgctaa atggcacggt gtacctcacc cttacggaca ccaacgcgaa
     1201 tgccacgttc tcggccagcg ccgcgttgaa cgtgccagcg gttgtgggtg gaaacgccgc
     1261 ctacgtcggg ttcaccggct ccgacggcgg cgctgaggcc aaccaagtca tcaacaactt
     1321 ccaattcatc agcttggtgc cgttgtcggc cacaccctcg aacgggaatc tggtcctcac
     1381 ctggcctctg ggcgtgggtg gttacgtcct ccagcaacgc tccagcctga actccggaag
     1441 ctgggccaat gtgccgaacg cagttaccac agtcggcggc atgaaccaag taaccgtgac
     1501 ggcaggcgcc gcgcagcagt tcttccaact cgtgttgcct taagcggaat acaaaacgga
     1561 tgattcacga ggccgggatt catttcccgg cctctttttt gaccttgctg tcgccttcat
     1621 tctatctcca aataaacttg cctgacggga cggccagctt catcctctcc cctttctaac
     1681 aatgatgcgt aaatcagctt tttttcgatt ctcgggtttc tccatcgtcg cggcctttgc
     1741 aataagtttg gctggccatg cgaccagggc agaccaagtc ccttcatttt tcggagagat
     1801 caaacctagc gtgcttccat tttccgcaac agtcacgaat gggaacatca ccttctcctg
     1861 gcagaccatg ccgggcaaat gggagttgct cgaacgacag cctgcggcct ccgtggaatg
     1921 gaaaccaatt cctcccagcc agtatcaaac caacggcttc atcgtctcca ccatcctgcc
     1981 gctgccgaaa cagaaggcgc tttattgcgt gaggcgaacg ctcggttttc gtccgcccaa
     2041 gctgccgaat cttcctcccg ttccgcctct gccgaccaat ttgccgccga ggttcaaggc
     2101 tcatccttga ccttgcgcgt ctcttttgat agtttcggcc cgcaagttca acaggtcgca
     2161 cccgaaatca gaccaccggg cggaaatcat ccgcacgggc aacaaaagga aatgacatgg
     2221 accggttcag aaggagttgg gatttgttca aaagttcatt gttcgtgatg atgcggcaca
     2281 agacgctgct cttgtttccc atcctcactg ccgtgctgac cgctttcctg ataattctat
     2341 tcctcgtccc ggtcgcattc cggccgacgg gttacagcta cacatcagcg cagcattgga
     2401 aagctgtgag caaaggcatc gtaaattccg attcaccatc gacccatcaa gattccccgg
     2461 aatcgggcag ctcgaattca aggctcaaca cgcttaaaaa ggtcaacccg attgcggcgg
     2521 tttacttcgc tgccatgtat tttgccacca tgctgctggc cacgttcctc aatgtggcgt
     2581 tttatcggga aatcttgaat gccttgagcg gccagccggt ttccatcgcc gaaggtctcg
     2641 gctttgccgc gcgcaaatgg aaaatcattt tgatgtggac gttgttcgcc ggacttgtcg
     2701 gtttcatcat caaaaccatg gaggaacggt tcggctggtt cggtgaaatc gtcatgcgcc
     2761 tcatcggcac cgtctggagc atcgcgtgcg tctttgtgat cccggtgatc atcaccgagg
     2821 agcagacctc caatccgttc gtcgttctca agaaatccgc tgagacactt aaaaatacct
     2881 ggggcgaatc attgattggc tatgccggtg tgagcgcggg cagttcggtg ctgatgattc
     2941 tttcgctctt ctggctcgcg gcagggcttg gcacggccat gtacttgcat tccgtgttgt
     3001 tgggtgtgct ggcggttgcg agctggctgt tcgcgctcgt ggtcatcagt tatttgatga
     3061 gcgttgccag ccagattttc cgctgcgcgc tgttccttta cgcttctcaa ggaacgctgc
     3121 ctcaccccta taacgaagag atgatggcgc tggcgtggaa gacgaagaaa agctgaagct
     3181 gttggcaacc tcggttgcac cgtgctgcgg acgcccggac gagcgccggg taatgaccta
     3241 cgcgtcatcc aatgcgggat gataatactt tgagccgggc acgttttgtt ttacaagcgg
     3301 cgggttgagc gaagccggcg gtggttccat ggattcgaca ctcgcggaaa tttcttcggc
     3361 gggattggaa tcgcccatcc cgtccaaaag actcgaacac gatgcgcaga tgttggggtt
     3421 cctggacaac gtatttcccc cctctgacat cggttcgcaa caacagatgc aaataatggg
     3481 cttcaacttc aaccaacatc aactcatctc aactgcattt caagaaaaat ttattcacat
     3541 tatttctaat ggctgtgaat aagttcaatg aatgattgaa acagaaccac ttccagcgga
     3601 attttcagtc tccgcggtaa acacaccgtg cggtgcacgt ttccgcgaca accacttccg
     3661 tcaattgcgg caacttcggt ttcaatttcc gccaaatcca cgccgcgaca ttttcgctgg
     3721 tcggattttc gagcccgcga atttcgttga gataatggtg atcaagctct tcccaaatcg
     3781 gtttgaatgc ttccgttatg tcggcgtaat ccatcaccca gccgagcttg ggatcgcatt
     3841 cgccggaaac gacgatctcc gccttgaaac tatgaccatg caaccggcga catttgtgcg
     3901 actttggcag atgcggcaac aggtgtgccg cctcgaattg aaacgtcttg cgtaaatcca
     3961 tcttcataaa ttctcttttt cccaatccgt ccacgtcacc aaatcggcaa gcggcggacg
     4021 tccatcgtgc cgccagagca acggcgggtt ctgcatctgg ccgagcggac agatgcgtgt
     4081 cacgccccaa cgggcaagtt gcgcggcgat tttgccggat tcttcctcgg tcgccaccag
     4141 tccgactgtt gaaaccttgc cgtgcacgga atcggcattt tgcaaggcat cggtgagatt
     4201 actcgccggt ttcacataga taaaccggtt caggcaggac agttgaaatc tcggatcagc
     4261 ctcgtaaatc accgtccagg cagtcgaatt ttcgctgcac caatgtcgcg tgctgtcggg
     4321 cgcatgcgcc gcgcggatct cataaaaagc tcgttttgag gcaatgatgg caacggattc
     4381 agcgggcaat tcaccgcgcg gttcggcggc ttcgcttcgc tccaattccc gggcgagcaa
     4441 ttcggcgaat tgttccgcgc cgatctcccc gccgttttca acgtagatga cgtggggtga
     4501 taaacaccca agctggttcc atgcgaccac atccgctgct gcgcgtgcgg cgatcttttt
     4561 cggattggcc cacgtgaacg cttccttcgt gatataacca aagctcacgc gatgaccgta
     4621 t
//
LOCUS       DASZMM010000588         5960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4933150, whole genome shotgun sequence.
ACCESSION   DASZMM010000588 DASZMM010000000
VERSION     DASZMM010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4933150"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VG754_14160"
     CDS             <1..304
                     /locus_tag="VG754_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20411.1"
                     /translation="AVQRVRAGIRAYNAAHKVPEGLTAGYHETITLAWMHLVDLTLCE
                     FGPEESADAFMDKHSQLLSKRALLFFWSRDRLMSADAKREFVPPDLAALPISVRKK"
     gene            304..783
                     /locus_tag="VG754_14165"
     CDS             304..783
                     /locus_tag="VG754_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF20412.1"
                     /translation="MKKVNTTNMEEFLWSSPKEKFVGAGKEISEELGRKPQSTDLLER
                     HPFDVEICRIPPGKILCPYHSHSAQWEFYHVISGNGAVRHKDGTTAIEPGDAFIFKPG
                     EPHQLTNDSAADLILYVVADNPIGESCHYPDSNKWSVPLPKRMLVRSEALDYFDGEE"
     gene            856..1551
                     /locus_tag="VG754_14170"
     CDS             856..1551
                     /locus_tag="VG754_14170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWF20413.1"
                     /translation="MFADFTVRWQRHELLPGRTMPLRIDYRRRHNARRYILRVNERGD
                     GGCVTIPRGGSWAEARRFALRNAAWLEKRLQQEKEKASQPQDCILFRGELLPLTEVFQ
                     RCFNCESARLIADENLRARVCLFLRNLAARELPTRVAELAPAHGLMVRRVMVRNQRSR
                     WGSCSAKGVISLNWRLVQTPEFVRDYIIVHELMHLREMNHSARFWNLVEAAFPRTDEA
                     ERWLKANGGLLRS"
     gene            1722..3575
                     /locus_tag="VG754_14175"
     CDS             1722..3575
                     /locus_tag="VG754_14175"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HWF20414.1"
                     /translation="MNRNHGRAILFAPGLVALVIFSCAFSYTRTIHVETTPPGATVYL
                     TNQPVGVTPLTQKIEFNKVASQIEVRVEKDGYAPESRVLTGQEAKDMDKRALWNINFE
                     LFETRRDIPVSITSPVAGSTVLLNGKPLGTVPLKRTLVFSRVKGGDWSAAALQVEKPP
                     YYHPSSRTISAEDAVEALKTSAEWQLNFPLAEIRRDLPIEIKANIEGASVSIDGQPLG
                     TAPVKHTLVFTRTDASSPWSEATLKVEKESFEHRPPGADGQPAYIRKITVDDAAGGTF
                     AATDLVPVRFVLTPVRMFEVQPEKILIVKTNLLSEVNEAEPGKPPTQITTAKAEAPLA
                     LCRISAVPDHPDQIAFTIPKREERVNPGDQADDEIVGANIWMVSGVAQTQMSEGRQFD
                     IDPFVTADGKWIYFSSNRLRTRAIWRMPANGKGGFTKITGDLSTVDTEPAVSPDGSKL
                     AYTSRSPNAMSLTPSYIWIANNDGSLPTQMRAGHSPAWSPDGNHIAFVSPENKIWVMD
                     TDGGNPTQLTLGDSIEANPVWTPTGKHIVFASDRAQNDLKQKNYDIWISDADGSKQTQ
                     LTSNGSFDASPAISSDGKYLYFFSNRGAQRTGQESLQIFRLELPSEYAPAK"
     gene            complement(3622..5169)
                     /locus_tag="VG754_14180"
     CDS             complement(3622..5169)
                     /locus_tag="VG754_14180"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWF20415.1"
                     /translation="MIALTLISMPCVASAAAPKPNIIVILVDDMGFSDIGCFGSEIPT
                     PNLDKLAATGLRFTQFYNTGRCCPSRAALLTGLYSHQAGIGHMTYDDHVPGYRGRLND
                     NCVTMAEVLGGAGYFTAMTGKWHVGQEHGVVPWKRGFERSLNSPAGGFYFPEGKPHRI
                     YLNGRALPDDSPELPTNWYTTDLWTDYGIRFIDEARAAKKPAFLYLAYNAPHFPLQAP
                     ADEIARFRGKYMIGWDKLREQRNAKQKQLGIVDPAWELSPRPDDVQAWEKLSDKEKDR
                     FDEIMSIYAACVAHIDTDIGKLVGALKERGMFDNTLIFFMSDNGGNAESGPNGRLEGD
                     HPGQAYSTVFCGKSWATLENTPFRRYKHFNHEGGVATPFIVHWPAGFSARGEFRQQPG
                     HLIDVMATCVDVSGAKYPAEFHGNAILPMEGRSLVPAFENKPIQRDALFWEHEGNAAV
                     RVGDWKLVRAGRRGQWELYDMKKDRTEMHDLSAEHPDIAKDLLPKWQAWAERAHVKPY
                     PAAKPAQ"
     gene            5321..5752
                     /locus_tag="VG754_14185"
     CDS             5321..5752
                     /locus_tag="VG754_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20416.1"
                     /translation="MQHSHDSEHILHGFFASGELRQALGYALFANRLKRRMDLDCSRS
                     SDRFAINLSMLGGSSEEFAYPNITELSIYFQLIVHFLSLRTNMMPPINWGLKKNIQPV
                     WMAKPILGESLCSGIRCKISGLIGRRKLACAGHSFLCTTPH"
BASE COUNT         1308 a         1657 c         1677 g         1318 t
ORIGIN      
        1 ggcggtgcag cgggtgcgcg cgggaatcag ggcgtataac gccgcgcata aagtgcccga
       61 aggtttgacg gccggttatc acgagaccat caccctggcg tggatgcatc tggtcgatct
      121 cacgctttgc gaattcgggc cggaagaaag cgcggacgct ttcatggaca aacactcgca
      181 actcctttcc aaacgcgcgc tgttattttt ttggtcacgc gaccgcctga tgtccgccga
      241 tgcgaaacgc gaatttgttc cgcccgattt ggcggcgttg ccgataagcg tcaggaaaaa
      301 ataatgaaaa aggtcaacac aacaaacatg gaagagttcc tctggtcgtc gccgaaagaa
      361 aaattcgtcg gcgcaggcaa ggagatctcg gaggaattgg gccgcaaacc gcagtccacg
      421 gacttgctgg agcggcatcc gtttgacgtg gagatttgcc gcatcccgcc gggaaagatt
      481 ctgtgtccct atcattcaca cagcgcgcaa tgggaatttt atcatgtgat ttcgggcaac
      541 ggcgctgttc gccacaaaga tggaaccacc gcaatcgagc cgggcgatgc ttttattttc
      601 aaacccggcg agccgcatca gttgacgaac gacagcgcgg cagatttgat cctttatgtc
      661 gtcgcggaca atccaatcgg cgaatcctgc cattatcccg acagcaataa atggagcgtg
      721 ccgttgccca agcgcatgct ggtccgctcc gaggcattgg attatttcga cggcgaggag
      781 tgatctgtcg ccttctgctt tcatctccgg ttgaaatttt tccataatcg tgcgcaaaat
      841 ccctttgcgc gatttatgtt cgctgatttc acagtccgtt ggcaaaggca cgaactcctg
      901 ccggggcgca cgatgccgtt gcgtattgat tatcggcggc ggcacaacgc gcggcgttac
      961 atcctgcgcg tgaacgagcg cggcgacggc ggttgcgtga cgattccgcg cggcggttca
     1021 tgggccgaag cgcgccggtt cgcgttgcgc aacgccgcat ggctcgaaaa acggttgcag
     1081 caggaaaagg aaaaggcatc gcaacctcag gactgcattt tgttccgtgg cgaattgctg
     1141 ccgttgacag aagtttttca aagatgcttc aactgcgaat ccgcaaggct gattgccgac
     1201 gaaaatttgc gcgcgcgggt ttgtctgttt ttgcgcaact tggcagcgcg ggaattgccg
     1261 acgcgcgttg ccgaactggc cccggcgcat ggactgatgg tgcggcgcgt catggtgcgc
     1321 aaccagcggt caagatgggg ttcgtgttcg gcgaagggcg tcatttcgct gaactggcgg
     1381 ttggtgcaga cgccggaatt cgtgcgcgat tacatcatcg tgcatgaatt gatgcatctg
     1441 cgggaaatga accattcggc gcgattttgg aatctggtcg aggcggcatt tccgcgaacg
     1501 gacgaagcgg agcgctggct caaggcgaac ggcggcttgc tgcgcagttg aggagatgga
     1561 gtattggggc gatggagaac tggagtgttg gggcgctgga ctcatcccat tactccactg
     1621 ctccatcact ccgtttccgc tttcgccgca caattgcgct tgttattttc ggaagagttg
     1681 tgtcagctaa agcgacacga gttagggctg caccaatctt tatgaaccgg aatcatggcc
     1741 gcgccatttt atttgcaccg ggcctcgtcg ccttggtgat tttcagttgc gccttctcct
     1801 acacccggac gatccatgtc gagaccacgc cgcccggcgc gacggtgtat ctcaccaacc
     1861 aaccggtggg cgtcacgccg ctgacgcaaa aaatcgaatt caacaaggtc gccagccaaa
     1921 tcgaagtccg cgtcgaaaag gacggttacg ctcccgaatc gcgcgtgctc acgggccagg
     1981 aggcgaagga catggacaaa cgcgccttgt ggaacatcaa tttcgaattg ttcgaaacgc
     2041 gccgggacat cccggtgagc atcacctcgc cggttgcggg cagcacggtc ctgctgaatg
     2101 gcaagccgct cggcaccgtg ccgctcaagc gcacgctggt gttctcgcgc gtcaaaggcg
     2161 gggattggag cgcggcagca ttgcaggtcg agaagccgcc gtattatcac ccatcttcgc
     2221 gcaccatcag cgcggaggat gccgtggaag cgttgaagac cagcgcggaa tggcagttga
     2281 attttccctt ggcggaaatc cgccgcgacc tccccatcga gatcaaggcc aacatcgaag
     2341 gcgcgtcggt ttcgattgat ggccagccgc tcggcaccgc gccggtgaag cacaccttgg
     2401 ttttcacacg cacggacgcc agctcgccgt ggtccgaagc gacgttgaag gtcgagaagg
     2461 aaagttttga gcatcgtcct cccggcgcgg acggccaacc ggcttacatc cgcaaaatca
     2521 ccgttgacga tgcagccggg ggaacatttg ccgcaacgga ccttgttccg gtgcggtttg
     2581 tgttgacccc ggtgcgcatg ttcgaagtgc agcctgaaaa aattctcatc gtcaagacga
     2641 acctgctttc cgaagtgaac gaggccgagc cgggcaagcc gcccacgcaa atcaccaccg
     2701 ccaaggctga agctccgctc gcgctttgcc gcatcagcgc ggtgccggac catcccgacc
     2761 agatcgcgtt caccattccc aagcgcgagg aacgcgtcaa tcccggcgac caggccgacg
     2821 acgaaattgt gggcgcgaac atctggatgg tttcgggtgt cgcgcaaacg caaatgagcg
     2881 aaggccgcca gtttgacatt gatcccttcg tgaccgcgga cggcaaatgg atttattttt
     2941 catccaaccg cttgcgcacc cgcgcgattt ggcgcatgcc cgccaacggc aagggcggtt
     3001 tcaccaagat caccggcgac ctttccacgg ttgacaccga accggctgtt tcgccggatg
     3061 gctcgaagct ggcctacact tcgcgctcgc cgaacgccat gtcgctcacg ccttcttata
     3121 tctggatcgc caataatgac ggcagcctgc ccacacagat gcgcgctggc cacagtcccg
     3181 catggtcgcc cgacggcaat cacatcgcgt ttgtatcgcc cgaaaataaa atctgggtca
     3241 tggacaccga tggcggaaac ccgacgcaat tgacccttgg cgattccatt gaagcaaatc
     3301 ctgtctggac gccgaccggc aagcacatcg tcttcgcgtc ggacagggcg caaaacgacc
     3361 tcaagcaaaa gaattacgac atctggattt cagacgctga cggcagcaaa caaacgcagc
     3421 tcacctccaa cggctcattt gacgcttcgc cggccatcag ctccgatgga aagtatctct
     3481 acttcttttc caaccgcggc gcgcaacgca ccggccagga atcgctgcaa atcttccgtc
     3541 tcgaactccc cagcgaatac gcgccggcga agtgaggagt gccgcaagat gccagatgca
     3601 tccaaaattg ccggcagatt tttattgggc gggtttggcg gcgggatacg gtttgacatg
     3661 ggcgcgttcg gcccaagcct gccatttggg gagcaggtcc ttcgcgatgt ccgggtgttc
     3721 ggctgagagg tcgtgcattt ctgtccggtc ctttttcatg tcataaagct cccattggcc
     3781 gcggcgtccc gcgcgaacca atttccaatc gcccacgcga acggcggcgt tgccttcgtg
     3841 ttcccaaaag agcgcgtcgc gttggatggg tttattttcg aaagctggaa cgagactgcg
     3901 gccttccatg ggaagaatgg cattgccgtg aaattccgcc ggatatttcg cgcccgacac
     3961 atccacgcag gtggccatga catcaatcaa atgaccgggc tgttggcgaa attcgccccg
     4021 cgcggaaaat cccgcgggcc aatgcacgat gaaaggcgtg gcgacgccgc cttcatgatt
     4081 gaaatgtttg taacgccgga aaggagtgtt ttcgagcgtc gcccaggact tgccgcaaaa
     4141 gactgtcgag taagcctgtc ccggatggtc accttcaagg cgtccgtttg gtccgctttc
     4201 cgcgttgccg ccgttgtcgg acatgaaaaa gatcaaggtg ttgtcgaaca tcccgcgttc
     4261 cttgagcgcg cccaccaact ttccgatgtc ggtgtcaatg tgcgccacgc aagcggcgta
     4321 gatggacatg atttcatcaa aacggtcctt ctccttgtcc gacaattttt cccaggcttg
     4381 cacgtcatca ggacgcggcg acaattccca tgccgggtca acaatgccca actgtttttg
     4441 tttcgcgttg cgttgctcgc gcagcttgtc ccagccgatc atgtatttgc cgcgaaaacg
     4501 cgcgatctcg tccgcgggcg cttgcagcgg aaaatgcggc gcgttgtaag cgagataaag
     4561 aaaggccggt ttttttgccg cgcgcgcctc gtcaatgaac cgaattccat aatctgtcca
     4621 gaggtcggtg gtgtaccaat ttgtcggaag ttcgggcgaa tcatccggca gcgcgcggcc
     4681 attgagataa atccggtgcg gtttgccttc cggaaaataa aatccgccgg cgggtgaatt
     4741 gaggctgcgc tcaaatccgc gtttccaagg gacaacacca tgttcctggc cgacgtgcca
     4801 tttgccggtc atcgcggtga aataacccgc gccgcccaag acttcggcca tggtgacgca
     4861 gttatcattc aaccgtccgc gataacccgg cacatgatca tcatacgtca tgtggccaat
     4921 accagcttga tgcgaataaa ggccggtcag caatgccgcg cgactgggac aacaccggcc
     4981 cgtgttgtaa aattgcgtga agcgcaaacc ggtggcggca agcttgtcca aattgggcgt
     5041 gggaatttca cttccaaaac aaccgatgtc cgaaaagccc atgtcgtcca ccaagatgac
     5101 gatgatgttg ggtttgggcg cggctgcgga cgccacacat ggcatggaga tcagcgttag
     5161 cgcaatcatc cccaaaaatg ttcgtcgaaa gcttctattg tttgttctat aggcagaggg
     5221 cataaaacta ttgttaatgg tttgaagaaa cgccagcaat aacaaaccag gcatgggagc
     5281 gattgccgtc acgatgccag attttttttg aaggcacgtt ttgcaacatt cccatgattc
     5341 agaacatatc ttgcatggat tttttgcatc cggcgaattg agacaagcct tgggctatgc
     5401 cctttttgct aatcgcttga aacggcgaat ggatttggac tgcagccgca gttcagacag
     5461 gttcgccatc aatttatcga tgctgggcgg ctcttcagag gaatttgcat atccaaatat
     5521 tactgaatta tcaatttatt ttcaattaat agttcatttt ttatcactga gaacgaacat
     5581 gatgcctcct ataaattggg ggttgaaaaa aaacatacaa cctgtgtgga tggccaagcc
     5641 tattcttgga gagagtctct gttcaggaat tcgttgcaaa atttcagggc tgataggccg
     5701 tcggaagctg gcttgtgcag ggcattcttt tctttgcaca accccgcatt gatggcgacc
     5761 actgtttccc atggccagtc cgccagccat cgtctcttca tttcgacttt gttatgcggc
     5821 ggcgaaggtt ggctttgatc ttttgaatgg tcacaatccc ctcgctttta agataaccgt
     5881 ggccggtctt cgcaaagagc ggttcggcaa caaatctgtc ctggatgcgc aatgcttctt
     5941 tcatcgcgtt gcgaaggttc
//
LOCUS       DASZMM010000589         8432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1094402, whole genome shotgun sequence.
ACCESSION   DASZMM010000589 DASZMM010000000
VERSION     DASZMM010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094402"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VG754_14190"
     CDS             complement(<1..489)
                     /locus_tag="VG754_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWF20417.1"
                     /translation="MKILEHTPEDLTATVEAGITLVTLQTELARHRQWLPVDPPHPER
                     LTIAELIDRNLSGPRRFGYGTVRDHLIGIKVALTDGTIIHSGGKVVKNVAGYDLMKLF
                     IGARESLGTVLETTFKLHPLPEMERIVQTRCETLEQADKAIEMVMHSDLAPVVLDLVN
                     AEC"
     gene            complement(498..1952)
                     /locus_tag="VG754_14195"
     CDS             complement(498..1952)
                     /locus_tag="VG754_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWF20418.1"
                     /translation="MQSDPPVLLTAPMLDALRAIVGGENIIHGEQELLVYECDAYTLE
                     KQLPNVVVLPRTTEEVAAVARFCAKNNLPIIPRGAGTSLSGTVLAVTGGVMIALTRMN
                     RILEIDYRNRRALVEAGCVNAWVTQAVKGGGVYYAPDPSSQSACTIGGNVATNSGGPH
                     TLKYGVTTNHVLGFEMVLPDGEIVWLGARPDGGEDVDGYDLRGAVVGCEGMFGIVTRI
                     LLQLIRAPQMFKTMLGVFENVDDASRTVSDIIAAGIVPGALEMMDQLITQAVEAAYHF
                     GFPLDAGAVLIVELDGLAAGLDRQAERVMEICRRNRAREVRLAKTDQERMDLWKSRKR
                     AFGAIGRLSPNFLTQDGVVPRSKLPEMMRFIKACSEKYGLRIPNVFHAGDGNIHPLIL
                     YDEREPAQVKRALAAGHDILEKCVELGGSVTGEHGIGVEKIDFMEKQFTREDLDAMRA
                     LRRVFDPENRCNPHKMFPGSKRCSDFAPKKQIAA"
     gene            2105..2611
                     /locus_tag="VG754_14200"
     CDS             2105..2611
                     /locus_tag="VG754_14200"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HWF20419.1"
                     /translation="MSKLRFRSVIEINNINPYVLVSAQRVARIRKGWRKPLPVRVHVN
                     GKPDKPWRINLMPIGHGSFYLYLHGDIRKASSTKVGDTVTVELEFDDEYRSGPAYPMP
                     LWFNDALNRNRSAKQRWSKLIPSLQKEILRYFSRLKTPEAQTRNLQRAMYVLSGFKGR
                     FLGRSWNE"
     gene            2705..3523
                     /locus_tag="VG754_14205"
     CDS             2705..3523
                     /locus_tag="VG754_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20420.1"
                     /translation="MIDFAKIRQQPTGRTVFKQLIANALDLFLMLYGLKCILFQHGKM
                     LLAVRGRVHTSLHLAPMSGTLAAIVGLQYAAFGLFIFLWDGSPPPENRGWLWRIGRGL
                     LRWGSLALALWCFFEMPDHDPEFLFQYNILWLIVFIAGFFGLQSFLIAMYAREQVKRE
                     LNAMQCRPMHILWRPLPYWLSPFWFLPPTGFRVIYCDPSGSIHKGYCYVYRSFRENPQ
                     WGNRRVRWLTDTVTRELPSTEVYVDSEIIRPKLKKWDASTEADNLLENPDEPSD"
     gene            3649..5205
                     /locus_tag="VG754_14210"
     CDS             3649..5205
                     /locus_tag="VG754_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF20421.1"
                     /translation="MRRCFAFLCGWLLVLTASGATPELGKDALRKLVKLPTIIFDSNW
                     TFDPERGFTLGSSAQDVSDEIARLRQQSNHEPADAAVQLHIGDLLTSLNDIASAQKAY
                     GRAAEFYRQRVDAQPGDAMLLCGFGRALQGAGKTEEAESVLRKAVDIAPNEWRCRVAL
                     GRFLDADARHNIEDAGLSASHVTLARKELAEASECYDHASVIAPNEGEIYFRRGMHRS
                     LRAMLLNEIRMSEGEQRDEVVGSSRQFSDAALADLQRASRLKPSDYALIGNVVLFEIY
                     TGCSHNGQMDWGDFSWSSLPEKSQHSIHDAETRLENLGQVPDPRQASGALEVLGILQG
                     PILHDSRSCILNLRRAVALEPSRDQAWDVLAGTLARMHLYDELLSVCQERIHQKESAR
                     GHLLLAKAYEKLRQWDDSENEIMESLRMAPNSFTANLAEAALVLRRSQDPDVLLDANS
                     WLARSERLLNDLPPMQRNQEAIIELTLTRSIYFALSDQLDEARQWAKAVINQDKNNPV
                     AQEILSAMDY"
     gene            5483..6514
                     /locus_tag="VG754_14215"
     CDS             5483..6514
                     /locus_tag="VG754_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20422.1"
                     /translation="MAEKAFDPIKSWFDAAEALGEYIGIRFGRLRPGSNDPEWMFMPH
                     CDYDGIGGFAEILRQRGAVLSKLPVIKHPSNPSWMPLIKSGPKLLKPRRALEWNIPSD
                     KIKLSTNAQPPSAVAWHVFDEIETSQIRRACRKGGFTVNSFLLKHVSKAVRPCLKDEA
                     SVIPWMVPVNLRGKVFRDRDTANFSAYVGVKIASYETIRDVHRKIYEALGRGEHWANW
                     YAYKSGHVLSHGMKKQMVTSGRCMAEWYLGSFSNLGDWDADKKLNSPGCLGGWLFSPP
                     VLRCQLVGAGCVTFQNRLSLTIQTHPELTTDVAVPRNWMQNWVREIEMDLSSLLSDAV
                     TTPARLAAA"
     gene            complement(6582..7673)
                     /locus_tag="VG754_14220"
     CDS             complement(6582..7673)
                     /locus_tag="VG754_14220"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWF20423.1"
                     /translation="MQSSPHPSVAKRGKYRHIALCFLPQMVCVLVAVGILEKGYWLLL
                     PILFLQVVVPLLDLITGWQDGAHFDKSDFSPVEESLLFWNVRLYALFYMTTVVYFAMS
                     IRQVSGVEGGLVLAASSLMGGVCFAAAHELLHAKTSLDQILQRITTAFLFYPHYKLIH
                     IRSHHVHAGTDEDENTAWFNESFYSYLYRTIPGSAIRSWHLEAKRVFNGKRSAEANLI
                     RNKMISYALGQLALLLVFYLLSGVWGLLFYVAQVIGAHIVLESVNYIQHYGLLRKRQD
                     GEYEKTGPEHAWDTYHLFSSYVTFRVGHHSYHHLAVKPYYLLVAEPQAPKLPVGYFWS
                     IPMVLLPPWWRRVINPRLTASAARVDAPT"
BASE COUNT         1956 a         2292 c         2270 g         1914 t
ORIGIN      
        1 gcactccgca tttaccaagt ccaacaccac gggcgctaaa tccgaatgca tcaccatttc
       61 aatcgccttg tccgcctgtt ccaatgtttc gcaacgcgtc tggacaatcc gctccatttc
      121 gggcaatgga tgcaatttga atgtcgtttc gagaaccgtt ccaaggcttt ctcgcgcccc
      181 gatgaacaat ttcatcaaat cgtaaccggc gacatttttc accaccttgc cgccggaatg
      241 gatgatggtg ccgtcggtca gcgccacctt gatgccgatg agatgatcgc gaactgtccc
      301 ataaccaaat cgtcgcggcc cgctgagatt tcgatcaatc aattcggcga tggtcaggcg
      361 ttcaggatgc ggcgggtcaa ccggcagcca ttggcgatgg cgtgccaatt ccgtttgcag
      421 cgttacgagc gtgatgcccg cctcgacggt cgcggtcagg tcttccggcg tgtgttccag
      481 gattttcatg cctgcgttca tgcggcgatc tgtttcttcg gcgcaaaatc cgaacaacgt
      541 ttcgagccgg gaaacatctt gtgcggattg caacggttct ccgggtcgaa cacacgtcgc
      601 aacgcgcgca tcgcatcgag gtcttctcgc gtgaattgtt tctccataaa atcaatcttc
      661 tccacgccga tgccgtgctc gccggtcacg ctgccgccaa gttcgacgca cttttccaag
      721 atgtcatggc cagcagccaa agcgcgtttc acttgcgcag gctcgcgttc atcataaaga
      781 atgagcggat ggatgttccc atcgcccgcg tgaaaaacat tcgggatgcg caggccgtat
      841 ttttcgctgc acgccttgat gaagcgcatc atctccggca atttcgagcg cggcaccacg
      901 ccgtcttgcg tgaggaaatt cgggctgagc cggccgatgg cgccgaacgc gcgtttgcgg
      961 cttttccaaa ggtccatccg ttcctggtcg gttttggcga ggcggacttc gcgcgcgcga
     1021 ttgcgccggc aaatttccat cacgcgctcg gcctgccggt ccaatcccgc cgccaacccg
     1081 tccagctcca cgatcaacac tgcgcccgca tccagcggaa aaccgaaatg atacgcggct
     1141 tcgaccgctt gcgtgatgag ctggtccatc atctccaacg cgcccggcac aatccccgcc
     1201 gcgatgatgt cgctcaccgt ccggctcgcg tcgtccacat tttcaaacac tcccagcatt
     1261 gtcttgaaca tctgcggcgc gcggatgagt tgcagcaaaa tgcgcgtgac gatgccgaac
     1321 attccctcgc aaccgaccac agcgccgcgc aaatcgtagc catccacatc ttcgccacca
     1381 tcgggccgcg cgccgagcca gacgatttcg ccgtcgggca ggaccatctc aaaaccgagc
     1441 acgtgattgg tggtgacgcc gtatttgagc gtgtgcggcc cgccggaatt ggtcgccacg
     1501 ttgcccccga tggtgcaggc cgattggctc gaaggatcgg gcgcgtaata aaccccgccg
     1561 cccttgaccg cctgtgtcac ccatgcattc acgcaaccgg cttcaaccaa tgcgcggcga
     1621 ttgcgataat caatctccaa aatccggttc atgcgcgtca acgcgatcat cacgccgccg
     1681 gtcacggcca gcactgtgcc gctcaagctc gtgcccgcgc cgcgtgggat gatgggcaga
     1741 ttgttcttcg cgcaaaaccg cgccacggct gcgacttcct ccgtcgtacg cggcaacacc
     1801 accacgttgg gcaactgttt ttccaacgtg taggcatcgc attcatacac cagcaattcc
     1861 tgctcgccgt ggatgatatt ttcgccgccg acaattgcgc gcaacgcgtc cagcatcggc
     1921 gcggtcaaga ggacaggcgg gtcggattgc atggcaaaag cgtaatggca gcggcaaaaa
     1981 ctgtcgaaga aaaagctcca tgagcatggt tggcatttga tcgttgcaaa tggaaagcgg
     2041 gccgacaaat gcttgaagaa attctgcaaa aattgaaatt tatgcaaata cagcgccctg
     2101 atgcatgagc aaattacgct ttcggtcagt gattgagata aacaatatca atccctatgt
     2161 gctcgttagt gcgcagcgag tggcgcgcat ccgtaaaggc tggcgcaagc cgcttcctgt
     2221 gcgcgtccat gtgaatggca agccggacaa gccgtggcgc atcaatctga tgcccattgg
     2281 ccatggcagt ttttatcttt acctgcatgg cgacatccgc aaggcgtcga gcaccaaagt
     2341 gggagatacc gtcacggtcg agctggagtt cgacgacgaa tacagaagcg gccccgcata
     2401 cccaatgccc ttgtggttca atgacgcgct caaccgaaat cgaagcgcca aacaaaggtg
     2461 gagcaagttg attccgagcc ttcagaagga gattttgcgc tatttttccc ggctgaaaac
     2521 acccgaggcc cagactcgaa atcttcagcg ggcgatgtat gttttatccg gattcaaagg
     2581 gcgattcctg ggcaggtctt ggaacgaata agcgagcgca accaagcctg aaaaatgtaa
     2641 agggtctaaa gagctggaat tgcgacaacg cgccgcatga agaattttgg atgaccaatg
     2701 gtccatgatt gactttgcaa aaatcaggca gcaaccaacc gggcgaacag tcttcaagca
     2761 attgatcgcc aatgcgctgg acctttttct catgttgtat ggcttgaaat gcattttgtt
     2821 ccagcacggc aaaatgcttt tggctgtcag aggaagagtc cacacgtccc ttcatcttgc
     2881 gccgatgagc gggacgcttg cggcaatagt cgggttgcaa tatgcggcat ttggtctgtt
     2941 cattttcctc tgggatggaa gtccgccgcc tgaaaaccgc ggctggcttt ggcgaatcgg
     3001 gcgcggcctg ttgcgttggg gaagcctggc gcttgcgttg tggtgctttt ttgaaatgcc
     3061 ggaccacgat ccggagttct tattccaata caacattctg tggcttatag tgtttattgc
     3121 gggttttttt gggctgcaat catttttgat tgccatgtat gcgcgagaac aggtcaaaag
     3181 ggaattgaac gcgatgcagt gcaggccgat gcacatctta tggcgaccgc tgccgtattg
     3241 gttgagtcct ttttggtttc tgcctccgac aggatttcga gtgatttatt gtgatccatc
     3301 cggttccatc cataaaggat actgttacgt ttaccgttca ttccgtgaaa atccacagtg
     3361 ggggaatcgt cgcgtcagat ggttgacaga cacagtcacc cgcgaattgc cttcgacgga
     3421 agtgtacgtg gacagtgaaa ttatccgccc aaaattgaaa aaatgggatg cttcaaccga
     3481 agctgataat ctgttggaaa atcctgatga accttcggat tgatcgcttg agggatctgt
     3541 cgaagaaaaa gctccgtaac gctacggaga aatttttttc gctctaaaag catttttaac
     3601 tcgcgtgcag cggaacgtga tgttatacac tggcgcattg tagttgttat gcgtcgttgt
     3661 ttcgcttttt tatgcgggtg gctgctggtg ctgaccgctt cgggggcgac gccggaactc
     3721 ggcaaggacg ccctgcggaa attggtcaag ctgccgacaa tcatctttga ctccaactgg
     3781 acgttcgatc cggaacgtgg tttcaccctc ggttccagcg cgcaagacgt ctccgacgaa
     3841 atcgcccgcc ttcgccagca gtcgaaccac gaacccgccg acgctgccgt gcaactgcac
     3901 atcggcgacc tgctgacttc tctcaatgac atcgcctccg cgcaaaaggc ttatggtcgc
     3961 gcggctgaat tttaccgtca acgcgtggac gcgcagccgg gtgacgccat gttgctgtgc
     4021 ggtttcggtc gcgcattgca aggcgcgggc aagactgaag aggccgaaag cgtgctgcgc
     4081 aaggctgtgg atatcgcgcc caacgaatgg agatgccggg tcgcgttggg ccgttttctc
     4141 gatgcggatg cgcgtcacaa tattgaagac gcggggcttt ccgcatcgca tgtgacgctt
     4201 gcgcgcaagg aacttgccga ggcgagtgaa tgttatgacc atgcgtcggt catcgcgccc
     4261 aatgaaggag aaatttattt ccggcgtggt atgcaccgtt ccttgcgggc catgctgctg
     4321 aacgaaatcc gcatgtcgga aggcgagcaa cgtgatgaag tggtcgggag tagccgccaa
     4381 ttttccgatg cggcgcttgc cgacctgcaa cgcgcaagcc ggttgaaacc gagcgattac
     4441 gcgctcatcg gcaacgtggt cttgtttgag atttacaccg gttgttcgca taatggccag
     4501 atggattggg gggatttttc ctggagttcg cttcccgaaa aatcgcagca ttcgatccat
     4561 gacgcggaaa cgcggctgga aaatctggga caagttcctg atccgcgaca ggcttccggt
     4621 gcattggaag tgctgggcat cttgcaggga ccgattttgc acgactcgcg cagttgcatt
     4681 ctaaatttgc gtcgcgctgt tgccttggaa ccttcgcgcg atcaagcgtg ggacgttttg
     4741 gcgggcacgc tggcgcggat gcatctctat gacgagctgc tttccgtttg ccaggagcgc
     4801 attcatcaaa aggaatctgc gcgaggccat ctgttgctcg ccaaggcgta tgaaaagttg
     4861 cggcaatggg acgattcgga aaacgaaatc atggaatcct tgcggatggc gccgaacagt
     4921 ttcaccgcca atcttgccga agccgcgctg gtgcttcggc gaagccagga ccccgatgtt
     4981 ttgctggacg cgaacagttg gctggcacgg agcgagcggc tgctgaatga cctgccgccg
     5041 atgcaacgca accaggaggc catcatcgaa ttgacgctga cccgcagcat ctattttgca
     5101 ttgagtgacc agcttgacga agcccggcaa tgggccaagg cagtcatcaa ccaggacaaa
     5161 aataatccgg tcgcgcagga aatcctgagc gcgatggact actgactttc aatttcaaat
     5221 ttcagattgg aaatttcaaa ttggttaaag gacttgctga caaggatgta aggatttagc
     5281 ggacttccca aatctcgggg ttttatgcag aatcaacacc gaatgtagcg ttgaaataag
     5341 cgcgagttat cgagaccagc ttgaaaagcg ttctttttga tgaaaatggc ataaaaaacg
     5401 ctctcaaacg agcttgcgcg gtctttgtag cgactatcag ttttattgac cgtttgcatt
     5461 taaaaccata aaaagcaagt gtatggctga aaaagcgttt gatccaatca agtcatggtt
     5521 cgatgcagcg gaagcgctgg gcgaatacat tggcattcgc tttggccgtt tgcgccccgg
     5581 ttcgaacgac cccgaatgga tgttcatgcc gcattgcgat tacgacggca tcggtggttt
     5641 cgcggaaatc cttcgccaac gcggcgctgt cctgtccaag ttgccggtca tcaagcaccc
     5701 gtccaacccg tcctggatgc cgttgatcaa atccggcccc aaacttttga aaccccgccg
     5761 tgcgttggaa tggaacatcc cttccgacaa aatcaaatta agcaccaatg cccaaccgcc
     5821 cagtgcggtc gcgtggcatg tctttgatga aatagaaacc agccagatcc gccgggcctg
     5881 ccgcaaaggc ggtttcacgg tgaattcatt cctgctcaag catgtttcca aggcagtgcg
     5941 gccctgtctc aaggatgaag cgtccgtcat cccatggatg gtccccgtca atttgcgggg
     6001 caaagttttc cgcgaccggg acacagccaa tttttccgca tacgtggggg tgaaaatcgc
     6061 ctcttatgaa accatccgcg acgtgcatcg caagatttac gaggccttgg gccgcggcga
     6121 acattgggcg aactggtatg cctacaaatc cgggcatgtc ctcagccatg gcatgaaaaa
     6181 gcagatggtc acctccggtc gttgcatggc cgaatggtat ctcggcagct tttcaaacct
     6241 cggcgattgg gacgctgaca aaaagctcaa tagtcccggt tgcctgggtg gctggctatt
     6301 ctcgccgccc gtgctgcgct gccaattggt cggcgcgggt tgcgtcactt tccaaaaccg
     6361 cctcagcctc accattcaaa cgcatcccga actgacgaca gacgttgcgg tcccccgtaa
     6421 ttggatgcag aactgggtcc gcgaaatcga gatggacctt tccagcctgc tctccgatgc
     6481 ggtgacaact cccgcccggc tggcagcggc ttaggacggg tcgaagcttc cgacaggctc
     6541 ttctgatttc acaagatgtt ccaccccgct ggatgacccc cttatgtggg cgcatcaaca
     6601 cgggcagccg aagctgttaa cctcgggttg atcacgcgcc gccaccaagg tggcagaaga
     6661 accatgggga tggaccaaaa atatccaacc ggcagctttg gcgcctgcgg ttcggcaacc
     6721 agcaaataat aaggctttac ggcaaggtga tgataagagt ggtgaccaac ccggaaagtc
     6781 acatagctgg agaacaaatg gtaagtgtcc cacgcatgtt ccggccccgt tttttcatat
     6841 tctccgtctt gccgctttcg gagcaaaccg tagtgctgaa tgtaatttac cgactccagc
     6901 acgatatgtg ccccgatgac ctgggccacg taaaacaaca atccccaaac gcccgaaagc
     6961 aaatagaaga caagcaggag ggccaactgg cccaaggcat aggaaatcat tttgttccga
     7021 ataagattcg cttccgctga ccgttttccg ttgaaaaccc gctttgcctc caaatgccag
     7081 cttctgatcg cgctcccggg aattgtccgg tagagatagg aataaaagct ttcattgaac
     7141 catgcggtat tttcatcttc atctgtcccc gcgtggacat ggtggctccg gatatgaatg
     7201 agcttgtagt gcggatagaa taaaaatgca gtggtgattc tctggaggat ttggtcgagg
     7261 ctggttttcg cgtgcaacaa ttcgtgtgcc gcagcaaaac aaacccctcc catcaaactc
     7321 gaagcggcca gtaccaatcc gccctcgact ccgctaacct ggcgaatgga catcgcgaaa
     7381 tagaccacgg tcgtcatgta aaagagggcg tagagtcgaa cgttccaaaa aagcaaagac
     7441 tcttccacag gggaaaaatc gcttttgtca aaatgtgcgc catcctgcca gcccgtgatc
     7501 aggtccagca gcggcacaac gacctgcagg aaaaggatgg gcagcaacaa ccaatatcct
     7561 ttctccaaaa tgccaaccgc gaccaggacg cacaccatct gcggtagaaa gcacaaggcg
     7621 atgtgccgat attttccccg tttcgcgacg cttggatgag ggctcgattg cataaaggta
     7681 agttcttgtt tttatgacga tgttgccttt gccaagttct tgatgacaat tcatgtattg
     7741 tcccattctc cagtcgccga tcatgccaaa agcaattcac cgaattcccg attgaggtcg
     7801 gctgcacacg aagcaaagtg gcgccaatgc cgaattgagc ggccagtgac gataccatgt
     7861 taagaaatat aaaaggacga gcattttcaa gcactaagtt tattatcggg cgcatctcag
     7921 tttcctgcac gacggggtaa ttgaacgttt cctccccctc cccatgaacc tgattttgca
     7981 gaagtatctg cacgacaata gattgcaaaa tcaggttcat ggtgaggggg acagcgatgt
     8041 gcgtcggccc atgaacacaa ttcgcaagag cccataaacg tgcaaaatag tgagatgcgc
     8101 ccggtgtgag tcattgatgc atttaggatt tgcttcgaat caccgttctg gcaaattgtt
     8161 ttgatgagcg gaatcgcttt catggtggat gagcacggtc gaaaaaccgc cgcagtgatt
     8221 gatctccgac agcatggccg ggcatgggaa gacttttatg atctattgca gtctgaatcc
     8281 cggaaacgcg agcctcgcga aagccttgaa tcagttaaac gccgtttaaa tctcaaacgg
     8341 cggcgcaatg gctgattaca gcatcacctt tgcccgttcg gcgcgcaagg aattggaaaa
     8401 ttgccaaccg ccgtcgctga ccgagttctg gc
//
LOCUS       DASZMM010000590         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1094997, whole genome shotgun sequence.
ACCESSION   DASZMM010000590 DASZMM010000000
VERSION     DASZMM010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094997"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VG754_14225"
     CDS             <1..918
                     /locus_tag="VG754_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF20424.1"
                     /translation="SPTRKRLESTLKNYRHFEADIRDMAAIEGIFKKFGNDIQAVVHT
                     AAQPSHDWAAREPHTDFGVNALGTLNLLEATRLHCPEAAFIFTSTNKVYGDTPNRLPL
                     AANEKRWEIEATHPYWNGIDETMSIDQTKHSLFGASKVAADVLVQEYGRYFGMKTVCF
                     RGGCLTGPGHAGTELHGFLAYLMKCTVTGKPYRVFGYHGKQVRDNIHSHDLVEAFWQF
                     LRAPRSGEVYNMGGSRHSNCSMLEAIDLCEQISGRKLDWTYVEDNRIGDHIWWISDVR
                     KFQSHYPGWKYRYDLNGILNEIHAACAEK"
     gene            976..1206
                     /locus_tag="VG754_14230"
     CDS             976..1206
                     /locus_tag="VG754_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWF20425.1"
                     /translation="MKYANRITFNPNQCGGRPCIRGMRIRVKDVLEMLAANVPESEIL
                     KDFPYLEPDDIRACLEYAAQEVDHPVLVATPQ"
     gene            1214..1543
                     /locus_tag="VG754_14235"
     CDS             1214..1543
                     /locus_tag="VG754_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWF20426.1"
                     /translation="MLFIIDAQLPPALAPWLIAKGHQAVHVADIGMNEAEDNAIWLHA
                     LALGAVILSKDEDFASRAIQGQGAPTIVWLRVGNCSNQALRAWFEPLLPDVLRQIQQG
                     ERLIEVR"
     gene            1540..2610
                     /locus_tag="VG754_14240"
     CDS             1540..2610
                     /locus_tag="VG754_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF20427.1"
                     /translation="MKILISGICGFVGSTLAARLKESLPDAEIFGFDNFIRPGSELNR
                     LELKRLGIRVQHGDVRNASDTASLPAADWVIDAAANPSVLAGVDGQTSSRQLVEHNLY
                     GTINLLEYCKTHRAGFILLSTSRIYAIQPLAALKVEAVNKAFRPVAKVGLPSGVSEAG
                     VDEHFSTQAPISLYGSTKLASEAMALEYGETFNFPVWINRCGVLAGAGQFGRPDQGIF
                     SFWINSWLRKRPLKYLGFGGNGFQVRDCLHPKDIVALLSKQFSASAAKTHPRIVNVSG
                     GTNSAMSLAQLSDWCAARFGPHQIQADGSARPFDIPWMVLNSALAGKIWNWQPETSLE
                     NIMDEIARHAEKHPEWLDISAP"
     gene            2607..3377
                     /locus_tag="VG754_14245"
     CDS             2607..3377
                     /locus_tag="VG754_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF20428.1"
                     /translation="MTEHAGNPALPPLTLLSVVVPARDEEGCIASMVEHLHIELKLHN
                     VPHEIVVVDDGSKDRTWEILQKVSKVIPNLHPVQNNGLHGFGRAIIYGLDHMKGDSVV
                     IMMADESDDCRDVVRYWKKLNEGFDCVFGSRFLRGGGVIDYPRIKLMLNRMANYFLKV
                     LFQIKLNDTTNAFKCYRKTVIDGCRPLISPHFNLTVEIPLKAIVRGYTWTVVPITWRN
                     RRTGEAKLKIKEMGSRYLFICLYVWLEKYFSRGDYKKK"
     gene            3374..>3970
                     /locus_tag="VG754_14250"
     CDS             3374..>3970
                     /locus_tag="VG754_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20429.1"
                     /translation="MTAILAHRANLNGPNPPEENSLAACARALAEGFGLETDLRRDAT
                     GAFYISHDAQPRTPENALEDFTELFRKHPEAELAINVKELGYETELIFLMNSGRLGKR
                     SFYFDFELLEPKVPGVAQRKLKSLSNSAGVRLASRLSDRNEPLAQCLSIPAEIVWGDE
                     FDRLWLTEAEVRAIKQAGRLLYMISPEIHRFDMETTRRR"
BASE COUNT          914 a         1130 c         1084 g          842 t
ORIGIN      
        1 agccccaccc gcaaacgtct tgaaagcacc ctgaaaaatt atcggcattt cgaggcggac
       61 atccgtgaca tggccgccat cgaaggcatc ttcaaaaaat tcggcaatga catccaggct
      121 gtcgtgcata cggccgcgca accttcgcat gattgggccg cgcgcgaacc ccataccgat
      181 ttcggggtga acgcgctcgg caccttgaat cttctcgaag ccacacgcct gcattgcccc
      241 gaagcggcat tcattttcac cagcacgaac aaggtttatg gcgatacgcc gaaccggctg
      301 cctctcgccg caaacgagaa acgttgggaa atcgaagcga cgcatcccta ttggaatggc
      361 attgatgaaa ccatgtccat tgaccagacg aagcattcgc tctttggcgc gagcaaggtc
      421 gcggcggacg tgttggtgca ggaatacggg cgttatttcg ggatgaaaac ggtttgtttt
      481 cgcggtggtt gtcttaccgg tcccggacat gccggaacag agttgcacgg ttttctcgct
      541 tacctgatga aatgcacggt cacgggcaaa ccgtaccggg tttttggcta tcacggcaaa
      601 caggtgcgcg acaacatcca cagccatgat ttggtcgagg cgttctggca attcctccgc
      661 gccccgcgct cgggcgaagt ctataacatg ggcggcagcc gtcattcgaa ttgctcgatg
      721 ctcgaagcga ttgatctgtg cgagcaaatc agcggacgca agctcgattg gacctacgtc
      781 gaggacaacc gcatcggcga ccacatctgg tggatcagcg acgtgcgaaa atttcaatcg
      841 cattatcccg gctggaaata ccgctatgat ttgaacggca tattaaacga aatccatgcg
      901 gcttgcgcgg agaagtgatt ggcaaaggta agcgtttgct tgtaaaacat gcggaaaaca
      961 gatagtttga accccatgaa atacgccaac agaatcactt tcaacccgaa ccaatgcgga
     1021 gggcggccct gcattcgggg aatgcgcatt cgcgtcaagg atgtgttgga aatgctggcc
     1081 gcgaatgttc cggagagcga aatcctcaag gatttcccat atctggaacc cgacgatatt
     1141 cgggcatgtc tagagtatgc ggctcaggaa gtggaccatc ccgtgttggt cgctacacct
     1201 caatgaggct tgattgctct ttatcataga tgcccagttg ccgcccgcgc ttgcgccttg
     1261 gctgatagcc aaagggcatc aagccgttca tgttgcagat attggcatga atgaggccga
     1321 agacaatgcg atatggctgc atgctctggc tctcggcgca gtcattcttt caaaggatga
     1381 agattttgca agccgggcaa tccaaggcca aggcgctccg acaatcgttt ggctgcgcgt
     1441 cggcaattgc tcaaatcaag cgttacgcgc atggtttgag ccgttgctcc cagacgtatt
     1501 gcgccagata caacaaggcg agaggctgat tgaggtaaga tgaaaattct aatctccgga
     1561 atttgcggat ttgtcggcag cacgcttgcc gcgcggctca aggaaagcct gccggacgcg
     1621 gagattttcg ggttcgataa cttcatccgg cccggcagcg aattgaaccg cttggaactg
     1681 aaacgtctcg gcatccgcgt gcagcacgga gatgtccgca acgcatcgga cacggcgtcg
     1741 cttccggcgg cggattgggt gattgatgcc gcggcgaatc cgagcgtgct tgccggagtg
     1801 gacgggcaga ccagcagccg ccaattggtc gagcataatc tctacgggac catcaacctg
     1861 ctggaatact gcaaaaccca tcgcgccggt ttcattcttc tcagcacgag ccgcatttat
     1921 gcaattcaac cgttggccgc attgaaagtc gaagcggtga acaaagcgtt tcggccagtt
     1981 gcgaaagttg gtttgccgtc gggcgtttcc gaggctggcg tggatgaaca tttttcgacg
     2041 caagcgccaa tctcccttta cggcagcacc aaactcgcat ccgaagcgat ggccttggaa
     2101 tatggcgaga cattcaactt cccggtctgg atcaatcgtt gcggcgtgct cgccggcgcg
     2161 ggccaattcg gccgccctga tcagggcatc ttcagctttt ggatcaattc atggctgcgc
     2221 aaacgcccgc tcaaatatct cggcttcggc ggcaacggtt tccaagtccg cgattgcctg
     2281 catcccaagg acatcgtggc gctcttatca aaacaatttt ccgcaagcgc ggcgaaaaca
     2341 catccccgca tcgtcaacgt gagcggcgga acgaacagcg cgatgtccct tgcgcaactc
     2401 agcgattggt gcgcggcgcg atttgggccg catcaaatcc aggccgacgg cagcgcgcgt
     2461 ccgttcgaca ttccctggat ggtcttgaat tccgcgcttg ccggcaaaat ttggaattgg
     2521 cagccggaga cttctttgga aaatatcatg gatgaaatcg cccggcacgc ggagaagcat
     2581 cccgaatggc tggacatttc ggcgccatga ctgaacacgc cggcaatcca gcgctcccgc
     2641 ctctgacgct tctttccgtg gtcgttcccg cccgcgacga ggaaggctgt atcgcctcga
     2701 tggtcgaaca tctgcacatc gaactcaagc tccacaatgt cccgcacgaa atcgtcgtcg
     2761 tggacgacgg cagcaaggac cgtacctggg aaatcctgca aaaggtcagc aaggtcatcc
     2821 ccaatttgca tccggtgcag aacaacggcc ttcacggttt tggccgcgcc atcatttacg
     2881 ggctcgacca catgaaaggc gattccgtcg tcatcatgat ggccgatgaa tccgacgatt
     2941 gccgcgacgt cgtgcgctat tggaaaaaat tgaacgaagg tttcgattgc gttttcggca
     3001 gccgtttttt gcgcggcggc ggggtcatcg attatccgcg aatcaagctc atgctgaacc
     3061 gcatggccaa ttattttttg aaagtgctgt tccagatcaa attgaacgac accaccaacg
     3121 ccttcaaatg ctatcgaaag accgtgattg acggttgccg gccattgatc tcgccgcatt
     3181 ttaatttgac cgttgagatt cccctcaagg ccatcgtgcg cggctacaca tggaccgtcg
     3241 tccccatcac atggcgcaac cgccgcaccg gcgaggccaa attgaagatc aaggagatgg
     3301 gcagccgcta tcttttcatt tgtctctacg tctggctgga aaaatatttc agccgcggcg
     3361 attacaagaa aaaatgactg ccatcctcgc tcaccgcgcg aatttgaacg gcccgaatcc
     3421 gccggaagaa aattcactcg ccgcctgcgc gcgcgctctg gccgaaggct tcgggttgga
     3481 aaccgatttg cggcgcgatg cgaccggcgc attttacatc agccacgacg cgcaaccgcg
     3541 cacaccggag aacgccctcg aagatttcac cgaactgttc cgcaaacatc ccgaagctga
     3601 actcgccatc aacgtcaagg aactcggtta tgaaaccgag ttgattttcc tgatgaactc
     3661 gggccggctc ggcaaacgca gtttctattt cgattttgag ctgctcgaac ccaaggtgcc
     3721 cggcgtggcg caacgaaagc tcaaatcgct ttcgaacagc gcaggcgtcc ggctcgcgtc
     3781 gcgattgagc gaccgcaacg aaccgctcgc ccaatgcctt tccattccgg cggaaattgt
     3841 ctggggcgat gaattcgacc gcctgtggct gaccgaggcg gaagtccgcg ccatcaaaca
     3901 agccggcaga ttgctctaca tgatttcccc cgagatccac cggttcgaca tggaaaccac
     3961 gcgccgccgc
//
LOCUS       DASZMM010000591         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3816314, whole genome shotgun sequence.
ACCESSION   DASZMM010000591 DASZMM010000000
VERSION     DASZMM010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816314"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VG754_14255"
     CDS             complement(<1..378)
                     /locus_tag="VG754_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20430.1"
                     /translation="MKSEIKIVLVFLLASTLNLRTSIASAFEGRITATLTRGGETETF
                     LYTIGTNQLRIERSETNWPYPQNLVDLDTGDLTLLFPQNRSFVRLKPNANDASLQMSP
                     FLGMPTPPGGLPPGVGPQTSLPPS"
     gene            648..2666
                     /locus_tag="VG754_14260"
     CDS             648..2666
                     /locus_tag="VG754_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HWF20431.1"
                     /translation="MQPGFLSRIIHGAFPCLALALLLTVRASAQTNATGMTNSLLPPP
                     STNKVVLPSSVPDPFEPFNRGSWALNRVLLADVVRPTARGYRLIVRKPVRTSIGNFGR
                     NIAYPVRLLNNLLQGKWSGARDETDRFMCNTFVGCVGFMDVATTLKIPKSDADFGQTL
                     GQWGWRPQVFLMLPIYGPSNERDTIGLAADTAANPLTYISPYSFDPTQMYTYFSPYTY
                     WSYSLMYNNLTDTVDDFARFAETEQDPYSEIQYAWTFVRENRVADFRVKGKQDPSSLE
                     TLETVFFKPKDPEFSNRGKTRSVYLPSTHREMKYTVWMQKEKAAVVYIVPGLGSHRLA
                     DTAVALAELVFDQGYSVVNVSSPYNYEFMREASTAAMPAYTPVDVHDLHVGLTTIHHK
                     LERIYPGQFGDKALMGYSMGAFESLFLAGVEQTNKSPLIKFDRYVGINTPVRLLYGVS
                     KLDEFYNAPMMWPASERNSNLENTFLKVAGVTQNSFTPSSTLPFDAVESKFLIGLVFR
                     LNLRDIIFTSQERHNMGILRQPVNLWRREPVYDEILQYSYQDYFQKFVIPYYKKRGID
                     LGTGDTLEKASNLRTYASSLAANPNIRLMVNRNDFLLPPEDLEWLQSILPPEHLHIFE
                     EGGHLGNLANPNVQKTIVGMLDGLQPVAPKTPTPKSSGPAKTGPVSLP"
     gene            complement(2754..>3072)
                     /locus_tag="VG754_14265"
     CDS             complement(2754..>3072)
                     /locus_tag="VG754_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWF20432.1"
                     /translation="IAIHAHCHAKSLMDPAFMARLARRLPGRNVTLLETGCCGMAGAF
                     GALASKYELSLQVARPLVEKIAAQPDDAVVVASGTSCRHQISHLASAHPKHMAELLAD
                     ALA"
BASE COUNT          708 a          855 c          831 g          678 t
ORIGIN      
        1 cgaaggcggc aatgaggttt gagggccgac gccaggagga aggccaccgg gcggcgtggg
       61 cattccaagg aagggggaca tttggaggga ggcgtcgttc gcattcggtt tcagacggac
      121 aaaactccgg ttttgcggga acagcagcgt gaggtcgccg gtgtcgagat ccacaaggtt
      181 ttgtggatat ggccagttgg tttcgctccg ttcaatccgc agttgattgg tcccaatcgt
      241 gtaaaggaag gtctccgttt cgccgccgcg cgtgagtgtc gcggtgatgc ggccttcgaa
      301 ggccgaggcg atggaagttc gcaaattgag agttgaggcc aacagaaaaa ccaggacgat
      361 tttgatctct gatttcataa tttgttcgga agctcaattc agatttgcag gtcaaactcc
      421 gcaattgatg acgacacaac ggcgctggaa acaggccttt acaagcaagg cttccttata
      481 taacgcatta aatttcagtg caatcaaatt caggctcaaa gagtaaaatg ccggttctgc
      541 cggaaagtgc ttgcgtaaac ccacttgcgc ggaaggatgc taactcgtaa tgtttctcgg
      601 ctcctttcaa gattgaaaag gaactggcga taactgccga actcagaatg cagcccggat
      661 ttttgtctcg aatcatccac ggagcctttc catgcctcgc gctggcattg ttgctaaccg
      721 tccgcgcgtc ggcgcaaacc aatgcaaccg gcatgaccaa ttcgcttttg cccccgccat
      781 ccaccaacaa agtcgttctg ccatcctccg ttccggaccc gtttgaacct ttcaatcgcg
      841 gctcgtgggc attgaatcga gtgcttctgg ctgatgtggt gagaccgact gcaaggggtt
      901 accggctaat tgtgcgcaag ccggtccgca ccagcatcgg caattttggc cggaacatcg
      961 cctacccggt gcgcctgctg aataatcttt tgcaaggcaa atggagcggc gcgcgcgacg
     1021 aaaccgaccg tttcatgtgc aataccttcg tcggctgcgt ggggttcatg gacgttgcca
     1081 cgacattgaa aatccccaag tccgacgctg acttcggcca gacactcggc caatggggat
     1141 ggagaccgca agtttttctc atgcttccga tctacgggcc aagcaacgag cgcgacacga
     1201 ttggccttgc cgcagacacc gcggccaatc cgctgaccta catcagcccg tattcctttg
     1261 acccgactca gatgtacacc tatttcagcc cctacacgta ttggtcatac agtttgatgt
     1321 ataacaatct caccgatacg gttgatgact tcgcccgttt cgccgaaacc gagcaggacc
     1381 cctattcgga aatccaatat gcatggacgt tcgtgcggga aaaccgcgtc gccgacttca
     1441 gggtgaaagg caaacaagac ccttcatcgc tggaaaccct ggaaaccgtt ttcttcaagc
     1501 cgaaggaccc ggaattttcc aaccgtggaa agacgcgttc ggtttacctt ccttccacgc
     1561 accgtgaaat gaaatacacc gtctggatgc aaaaggagaa agcggcggtg gtttatatcg
     1621 ttcccggtct cggttcgcat cgccttgcgg acaccgcggt cgcgttggcg gaattggttt
     1681 tcgaccaggg ctactcggtc gtgaacgtca gcagccccta caattatgaa ttcatgcgcg
     1741 aagcctcaac cgccgcgatg cctgcttaca cgccggtgga cgtccatgac ttgcacgtcg
     1801 ggctgacaac gatccatcat aagctggaac gcatctatcc gggccaattc ggcgacaagg
     1861 ctttgatggg ttattcgatg ggagcgttcg aaagtctttt tctcgccggc gtcgaacaaa
     1921 cgaacaaatc gccgctcatc aagtttgacc gttacgttgg catcaatact ccggtgcgat
     1981 tgctctacgg tgtatccaag ctggacgaat tttacaacgc gccaatgatg tggcccgctt
     2041 ccgaacgcaa ttcaaatctc gaaaatactt tcctgaaagt cgccggtgtc actcaaaatt
     2101 cattcacgcc cagttccacg ctgccatttg acgcggttga gtccaaattt ttgatcggcc
     2161 tggtctttcg cctgaacttg cgcgacatca ttttcaccag ccaggagcgg cacaacatgg
     2221 ggattctccg gcaacccgtc aacctgtggc gtcgtgaacc ggtttatgac gaaatcctcc
     2281 agtattcgta tcaagattac ttccagaaat tcgtcatccc ttattataag aaacgcggca
     2341 tcgaccttgg cacgggcgac acattggaga aggccagcaa cctgcgcact tacgcgtcaa
     2401 gcctcgccgc gaacccgaac atccggctca tggtcaatcg caatgatttt ttgctgccgc
     2461 cggaggactt ggaatggttg caaagcattt tgcctcctga acacctccat attttcgaag
     2521 aaggcggtca cttggggaat cttgccaatc cgaatgtgca aaaaaccatc gtgggaatgc
     2581 tggatggatt gcagccggtc gcgccgaaaa cgccaacccc gaagtcgtcc ggcccagcaa
     2641 agaccgggcc tgtttctctt ccgtgaccgc ccgcgtcatc acgaaaacca ccaaactttg
     2701 aagtccaatt gattcctgtc ggaaagatgc gcaccagcaa agccgactca cggctaagcc
     2761 aacgcatcgg caagaagttc ggccatgtgt ttgggatggg cgctggcgag atgggaaatc
     2821 tgatggcggc agcttgtgcc ggaggcgacg acaaccgcat cgtcgggttg cgcggcaatc
     2881 ttttcgacga gcgggcgcgc gacttggagt gagagttcgt atttggaggc aagcgcgccg
     2941 aatgcgccgg ccatgccgca acagccggtc tccagcagcg tgacgttgcg cccgggcaga
     3001 cgccgcgcga gccgcgccat gaacgccggg tccatgagcg acttggcgtg gcaatgcgca
     3061 tggatggcaa tg
//
LOCUS       DASZMM010000592         7173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_1096372, whole genome shotgun sequence.
ACCESSION   DASZMM010000592 DASZMM010000000
VERSION     DASZMM010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096372"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VG754_14270"
     CDS             complement(<1..192)
                     /locus_tag="VG754_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HWF20433.1"
                     /translation="MPANRFNAFTDYGIGIGLRIPHYRHILKEKPVVDWFEIISENFM
                     VEGGRPLEILDSILEQYRVV"
     gene            complement(290..604)
                     /locus_tag="VG754_14275"
     CDS             complement(290..604)
                     /locus_tag="VG754_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20434.1"
                     /translation="MKRTTRTLIATVAITGLLSGTAINQARARDDAGNKDSATAPGKE
                     AKKMPKVQDCAGKNDCKGIGGCKTDSHGCKFKNDCKGKGGCSITKADIKKWEKMQKEK
                     KS"
     gene            complement(969..2129)
                     /gene="eboE"
                     /locus_tag="VG754_14280"
     CDS             complement(969..2129)
                     /gene="eboE"
                     /locus_tag="VG754_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HWF20435.1"
                     /translation="MQLNHGLHLAYCTNIHRGETWRETFNALQTHTLAVRRQICPDKP
                     YAIGLRLSNRAARELNEPSTFNAFQKWLEQNNCYVFTINGFPFGQFHGARVKEQVYLP
                     DWTSPERLAYTNLLFDLLARLVPPGVEGSVSTLPGSFKEFITSAAQQREIRDNLWRCV
                     EHTAKVSDTSGSKLHLGVEPEPLGWFETSAETIRFFEQMRAEHPNDARLDEHLGVNYD
                     TCHLAIEFEEPHSAITALRKNGIKISKLHLSSALKVRPTAEVRRELAAFADDIYLHQV
                     VARSAKGERHVYKDLAPALNDSATNAAEWRVHFHIPLHSPSTAWYENTSDHVEGILDL
                     LKAEPSLCSHLEMETYTWEVLPPALKNRSVVDQLVAEYEWILKRLAERGLVP"
     gene            complement(2143..3144)
                     /locus_tag="VG754_14285"
     CDS             complement(2143..3144)
                     /locus_tag="VG754_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF20436.1"
                     /translation="MNSDKLSELHIPAEDRKRPQRPLWIIFIAVILVTGIALYLAWPR
                     ASDLVRLQNGKKNGSTNAVAAVPAADSNAVAPPTISATNASTPDDVVLTVSGYIINRE
                     RIELSPRFMGTVNWIGVKKGDVVKKGEVVVLLDDTEYKARLHEAEGHLATAKAMVARA
                     KLDYDRIVQLNKTQTESQKAEDDARLQLQADEAAITEAEGSYEVAKTYVDWTVIRSPI
                     DGVVLEKLVQPNELVMPQSFGGAHGPSTALIAVADPKDLQVEIDLSEADLSKVSMGQK
                     CRISPEAYPDKVYQGRVAEIAPEADRQKGTLQVKVQIQEPDHFLTPELSAKVEFLKQ"
     gene            complement(3295..4458)
                     /locus_tag="VG754_14290"
     CDS             complement(3295..4458)
                     /locus_tag="VG754_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF20437.1"
                     /translation="MALPLTYNIRNVLIRWRATMATILGVALVVAVFVLVQSMAAGLE
                     KASANTGDPRNLLVVRKGSTAESSSQVKLDQFKLIQYMPGIAHDAEGRPLVSADVIVL
                     ISLPRRENQGEANIMVRGVSPMGQKLRPQVALVEGRWFVPGKREVVVSRRLARRFANF
                     EIGSKFKTGGKELTVAGIMDAGNSAFDSEIWMDADEARSIFDRENYSSVLVRAENPAA
                     EKALADRIEADKRLPLRGLPESEYYASQTMTAVPIKILGDFLAIAMSIGATFAAMNTM
                     YASVGSRTREIGTLRVLGYRRTSILVSFLIEGAFLALIGGALGCVLALPMHGYSVGTM
                     SFETFSETVFQFRITPLLAFKGMVFSIIVGLVGSLLPAIRAARLPVIAALKSV"
     gene            complement(4621..5766)
                     /locus_tag="VG754_14295"
     CDS             complement(4621..5766)
                     /locus_tag="VG754_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWF20438.1"
                     /translation="MTIGSFILKNALRNKRRATLSVLSVAVSLFLLVTLLVALRELTI
                     PAEGEGAALRVVVRNKISIANPLPARQRPIIERIPGVEAVSPFTWFGGKFKNEEAMTF
                     AQFAMDEKQVGKIFVEAKVAPEEYEKFVSDRQACIVGKITADKNHIKVGDRIVLTSEY
                     YACTLELKVVGIYSGTVDDRNLFFHQKYLDESIGDPGTVGTWWVKVKTADDMPKVISA
                     INHAFANTSAEVRAESERAFQLSFMSMWGNIKLFVNSICTVVVFTLLLVSASTMSMAI
                     RERFRELAILKALGFRRRELFAFILAESFGLAVLGAFLGIGGAYCLYTYGNIAKMTQG
                     IFIMFEVTPKIIGTGALVAVGLGIIASIAPSVAVARMSVVKGLKTLD"
     gene            6160..6849
                     /locus_tag="VG754_14300"
     CDS             6160..6849
                     /locus_tag="VG754_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20439.1"
                     /translation="MGKAIIITKITAIAGAVLIAISASATPKLDLNVGGSGTDVVGEI
                     LPGYIGAGANDSAGQAADESSDANAMIPMALGTSQNITTSKGVSYVYRSLNNFGTLPY
                     AVVTGNAVLASGGGMTQVGHEIEISIGASGFTYLVARWDGPNAGAEVWDISGIANTDI
                     YIPADAAPDSTGTNLIATTGQYGITSYSLFQGSTNPPPPPSVPEVASTLALLGGAVAS
                     LTFLRKKISSK"
     gene            complement(6916..>7173)
                     /locus_tag="VG754_14305"
     CDS             complement(6916..>7173)
                     /locus_tag="VG754_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF20440.1"
                     /translation="HLPRQLSGGQEQRVAIARALVTSPALIVADEPTGNLDSQSAHDA
                     LGLLQTLSQTAGKTVIMVTHDPKAAAFGSRTIHLEKGELSN"
BASE COUNT         1596 a         1922 c         1988 g         1667 t
ORIGIN      
        1 caccacgcga tattgttcga gaatgctgtc gagaatttcc agcggacgcc cgccttcgac
       61 catgaaattt tccgagatga tctcgaacca gtccaccacc ggtttttcct tgaggatatg
      121 ccggtaatgc gggatgcgca ggccgatgcc gatgccataa tcggtgaacg cattgaaacg
      181 attggcgggc atcggaaaaa gttccgggtt caaagttccg agttccgagt tggcgaacgc
      241 ccggttcttt tcccaacccg gaaccctgaa ctcggaaccc ggaacgaaat cagctttttt
      301 tctccttctg catcttttcc cactttttga tgtctgcctt ggtgatgctg caaccgccct
      361 tgcctttgca atcgttcttg aatttgcagc cgtgggagtc ggtcttgcaa ccgccgatgc
      421 ctttgcaatc gttcttgccg gcgcaatcct gcaccttggg catcttcttg gcttccttgc
      481 cgggagcggt tgcgctgtct ttgttcccgg catcatcgcg ggcgcgggcc tggttgatgg
      541 cagtgccgct gagcaagccg gtgatggcga cagtggcgat aagagtgcgt gtggttcgtt
      601 tcatagatgg agtttaaact ggtttaatgg ccggctttaa ttgactgcat cattcccacc
      661 tgttcaagtg gaaaatgctg aaagctctgc tattcggcgc atctcagttt cctgcacgac
      721 ggagtgattg aacgtttgct ccccctcacc ccagccctct cccttgggga gagggagcaa
      781 cgcatcccgt cgtggttgca atctgacggc gtttaatttg tcgagcagcg gccgaatggt
      841 tccccctctc cttggggaga gggctggggt gagggggacc gcgatgtgcg ccggcccatg
      901 aacacaattc gcaagagcca gaaacgtgca aaatagtgag atgcgcccca gacaactcac
      961 gttttcagct acggaaccaa tccgcgttca gcaaggcgct tcaagatcca ctcgtattcc
     1021 gcgacaagct ggtccacgac gctgcggttt ttcaacgcgg gcggcaggac ttcccacgtg
     1081 taagtttcca tttcgagatg ggaacaaagc gacggttcgg ccttcaacaa atcgagaatg
     1141 ccttcgacgt ggtcggaggt gttttcgtac catgcggtgg aaggactgtg caacggaatg
     1201 tgaaaatgca cgcgccattc cgcggcgttt gtggcggaat cattcaacgc aggcgcaagg
     1261 tccttgtaaa catgccgttc gcccttggct gaacgagcaa cgacttgatg cagataaata
     1321 tcgtcggcaa atgcggcgag ttcgcggcga acttcggctg ttggccgcac tttcagcgca
     1381 gaactaagat gaagcttgct gattttgatc ccattcttgc gcaacgctgt gattgcggag
     1441 tgcggctctt caaattcaat cgcgagatgg caggtgtcgt aattgacgcc gagatgttca
     1501 tcgagccgcg cgtcattcgg atgttccgcg cgcatctgct cgaaaaatcg gatggtttcc
     1561 gcgctggttt caaaccagcc gagcggctcc ggttccacgc cgagatgcaa tttacttcct
     1621 gacgtgtcgc tcaccttcgc ggtgtgctcc acgcaacgcc atagattgtc gcggatttcg
     1681 cgttgctgcg cggcgctggt gataaattct ttgaaggagc cgggtaatgt gctgacgctg
     1741 ccttcaacgc ccggcggcac aagccgcgcg agcaaatcga acagcagatt cgtgtaagcc
     1801 aggcgctcgg gcgaagtcca atcgggcaga taaacctgtt ctttcacgcg cgcgccatgg
     1861 aattggccga agggaaatcc gttgatggtg aaaacgtagc agttgttttg ttcgagccat
     1921 ttttgaaacg cgttgaatgt cgaaggctca ttcagttcac gcgcggcgcg gttgctcagg
     1981 cgcaagccga tggcgtaagg cttgtcgggg caaatttgcc ggcgaacggc gagcgtgtgt
     2041 gtttgcaagg cgttaaatgt ctcgcgccaa gtttcgccac gatggatgtt cgtgcaatag
     2101 gcgagatgaa ggccgtggtt cagttgcatc gtggcgaatt ctttattgct tcaaaaattc
     2161 caccttcgcg ctgagctcgg gcgtgagaaa atggtccggc tcctggattt gcaccttcac
     2221 ctgcaacgtt cccttttggc ggtcggcttc gggggcgatc tccgcgacgc ggccttggta
     2281 aaccttgtcc ggataagcct cggggctgat gcgacatttc tggcccatcg aaactttcga
     2341 caaatcggct tcgctcaagt caatctcaac ctgcaaatcc ttcgggtcag ccacggcaat
     2401 caacgcggtg cttggaccat gcgcgccgcc aaaactttgc ggcatgacga gttcattcgg
     2461 ttgcaccaat ttttcgagca ctacgccatc aattggcgaa cggatgaccg tccagtccac
     2521 gtaggtcttg gcgacttcat aactgccttc ggcttccgtg atcgcggctt cgtcggcctg
     2581 taactgcaac cgcgcgtcgt cctcggcctt ttgcgattcg gtttgcgttt tgttgagctg
     2641 aacgatgcgg tcataatcca gcttcgcgcg cgcaaccatg gccttggcag tggcaagatg
     2701 accttccgcc tcgtgcaatc gcgccttata ttcggtgtca tccaacaaca caaccacctc
     2761 gccttttttg acaacgtcgc ctttcttcac gccaatccaa ttcaccgtgc ccatgaaacg
     2821 cgggctcagt tcgatgcgtt cacgattgat gatgtagccg ctcaccgtca gaacaacatc
     2881 gtccggagtg gaagcgttcg tggcggagat tgttggcgga gcgactgcat tagaatctgc
     2941 ggcgggaact gcagcaacgg cattggtcga accatttttt ttgccgtttt ggagacgcac
     3001 caggtcggac gcgcggggcc aggccagata aagcgcgatg ccggtgacaa gaatcaccgc
     3061 gataaaaatg atccaaagcg gccgttgagg acgcttgcgg tcctccgccg gaatatgcag
     3121 ctcgctaagt ttatccgaat tcatttcaac gtggtaagat gggggcaaat gggcttgcgg
     3181 gaaaggcaga aatttggaga cgctgggtat cgaaatattg gagtgctgga gtaatggagt
     3241 gttgcgtaaa gacgcattct acctacaaat ccaatctccc gttgttccga ttcttcacac
     3301 ggacttcaat gccgcaatca ccggcaaacg cgctgcgcga atggccggaa gcaaactgcc
     3361 gaccaagccc acgatgattg agaacaccat gcctttgaac gccaaaaggg gcgtgatgcg
     3421 gaattgaaaa acggtttcgc tgaaagtctc aaaactcatc gtgccgactg aatatccgtg
     3481 catcggcaat gcaaggacgc aaccgagcgc gccgccaatc aacgccagaa acgcgccttc
     3541 aatcaaaaaa cttacgagga tggaagtgcg ccggtaaccg agcacgcgca acgtgccgat
     3601 ttcgcgcgtt cgcgagccga cgcttgcata catggtgttc atcgccgcga acgtcgcgcc
     3661 gatggacatc gcgatggcga gaaaatctcc caggattttg atcggcacgg cggtcatcgt
     3721 ttgcgaagcg taatattcgc tctcaggaag cccgcgcaat ggcaggcgtt tgtcggcttc
     3781 gatgcggtca gccagcgctt tttctgccgc gggattttcc gcgcgcacga gcacgctgga
     3841 ataattttca cggtcaaaga tcgagcgcgc ttcgtcggca tccatccaga tttccgaatc
     3901 gaaagcgcta ttgccggcgt ccatgatgcc tgcgaccgtg agttccttgc cgccggtctt
     3961 gaattttgag ccgatctcaa aattggcgaa tcgtcgcgcc agccggcgac tgacgaccac
     4021 ttcgcgtttg cccggcacga accaacggcc ttccaccagc gcaacttgcg ggcggagctt
     4081 ctgtcccatt ggcgaaacac cgcgaaccat gatgtttgct tcgccctgat tttcacggcg
     4141 cggcagcgag atgagcacga tgacatcggc ggaaacgagc ggacggcctt cggcatcatg
     4201 cgcgatgccg ggcatgtatt ggatgagctt gaattggtcg agcttgacct ggctgctgga
     4261 ttcagcggtc gagcctttgc gtacaaccag aagattgcgc gggtcgccgg tgttggcgct
     4321 ggccttttcg agaccggcag ccatggattg caccaaaaca aaaacggcaa caaccagcgc
     4381 gacgcccagg atggtggcca tcgtggcgcg ccaacggatg aggacgttgc ggatgttgta
     4441 tgtgagcggc aaagccattt tggatttttg attttagatt tacgatttac gagctgtgtg
     4501 cgacatcaat tatcgttgca ggagccgacg tgaggaggtt ttctttggat ttcaaatctg
     4561 aaatttgaga tttcaaatcc aaacggaaac gaccaaaaca gaggccctgc aaattaatag
     4621 ttaatccaat gttttcaatc ctttcaccac gctcatgcgc gcgacggcga cggacggggc
     4681 gatgctggca atgatgccca atcccaccgc caccaatgcg ccggttccga tgattttcgg
     4741 cgtgacttca aacatgatga aaatgccctg cgtcattttt gcgatgttgc catacgtata
     4801 aagacaatac gcgccgccga tgccgagaaa cgcgccaagc accgccaacc caaaactctc
     4861 ggcaaggatg aacgcgaaaa gttcgcgccg ccgaaaaccg agcgccttca aaatggccag
     4921 ttcgcgaaac cgttcgcgga tcgccatgct catggtgctg gcggaaacga gcagcaatgt
     4981 gaacacgaca actgtgcaga tggaattcac aaacaatttg atgttgcccc acatcgacat
     5041 gaagctgagc tggaacgccc gctcgctttc ggcgcggact tcggcggaag tgttggcaaa
     5101 ggcgtggttg atggcggaaa tgaccttcgg catgtcgtct gccgtcttga ccttcaccca
     5161 ccaggtgccg accgtgcccg gatcgccgat ggactcatcc aaatattttt ggtgaaagaa
     5221 aaggtttcgg tcatccacgg tgcctgaata aatcccgacc actttcaatt ccagcgtgca
     5281 ggcataatac tcgctcgtaa ggacgatccg gtcgcccacc ttgatatggt ttttgtccgc
     5341 ggtgattttg ccgacgatgc aggcttggcg gtccgacaca aatttttcat attcttcagg
     5401 cgccactttg gcttcgacaa aaattttgcc gacctgtttc tcgtccatgg cgaattgcgc
     5461 gaaggtcatc gcctcctcgt tcttgaactt gccgccaaac caggtgaagg gcgaaaccgc
     5521 ctcgactccc gggatgcgct cgatgatcgg ccgttgacgc gcaggcaacg gattggcgat
     5581 ggaaatcttg ttgcgcacca caacgcgcaa cgccgcgcct tcgccttccg ccgggatggt
     5641 caattcacgc aacgcgacca gcagcgtcac cagcaaaaac aaactgaccg ccacgctcaa
     5701 caccgagaga gtggcgcgac gtttgttgcg caaagcattt tttaaaatga agctgccgat
     5761 tgtcatttaa agaccggtgc agaatccgtt gtattagaat tcgtaagaac ctactgctaa
     5821 ttgtgttgga gaaaagcctg aaatcgtaat gacggcgcac ggtatttcct tacaaaacaa
     5881 gttatgtttt atatgatgct cataatgagc agtttagatt taaatatgcg ttttgcgcga
     5941 ttcaaaacgt cggaaaagct tacaaaaagc gtcgaaaaca ctttttagat gaaaaaaaca
     6001 aaacatgggc aatagattgc aaatgaggaa tttatacctg ctcaaagctc gcggttcttt
     6061 ccatggcatg gcggctgctt cttcatggca tggcggctgc ttcttagctg gaaatagtaa
     6121 taacgaaatt cagcggatca acctaaataa gaggtaatta tgggcaaagc aattattata
     6181 acaaaaatca ctgcaatagc cggagcggta ttgatcgcga tttcggcgag cgcaacccca
     6241 aaacttgatt tgaatgtggg cgggagcggc accgatgtgg tcggtgaaat cttgccgggt
     6301 tacattggcg cgggtgccaa cgattccgcc ggtcaggcag cggatgaatc gtcggacgcg
     6361 aatgcgatga ttcccatggc cctcggcacc tcgcaaaaca tcaccaccag caagggagtc
     6421 tcctatgttt accgttcgct caacaatttt ggcacccttc cttatgcggt cgtgacgggt
     6481 aacgcagtgc ttgcctccgg cggcggcatg acacaggtag gccatgaaat cgaaatctcg
     6541 atcggcgcca gtggctttac ttacttggtg gcaaggtggg acggcccgaa cgccggcgcg
     6601 gaagtttggg acatttctgg tatcgcgaat actgacattt acatcccggc agatgctgca
     6661 ccggattcga ctggcaccaa cttgatcgcc acgacgggcc agtacggcat tacgtcgtat
     6721 tccctctttc agggcagcac caaccctcct cctcctcctt cggttccgga agtggcttcg
     6781 accttggcgc tattgggcgg tgcagttgcc agcctgactt tccttcgcaa aaagatcagc
     6841 agcaagtaag ctcgcaattc aatttcaggg cgcagagatt cgtctctgcg ccttttttat
     6901 ttggttcctt gccgctcagt tggacagttc gcctttttcg agatggattg tccgtgaacc
     6961 gaacgcggcg gctttggggt cgtgcgtgac catgatgacg gttttgccgg cggtttggct
     7021 gagtgtttgc agcaggccga gggcatcatg ggcggattga gaatccaggt tgccggtcgg
     7081 ttcgtcggca acaatcagcg cgggcgaagt gaccagcgcg cgggcgatgg caacccgctg
     7141 ttcttgtcca ccggaaagct ggcgcggcaa atg
//
LOCUS       DASZMM010000593         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2469841, whole genome shotgun sequence.
ACCESSION   DASZMM010000593 DASZMM010000000
VERSION     DASZMM010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469841"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VG754_14310"
     CDS             complement(<1..384)
                     /locus_tag="VG754_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20441.1"
                     /translation="MDIVFNCSKCGVELEVDASGVGEEINCPRCNTKLRIPEANAPGV
                     SIAEPQTGDGSSRWGGPAVNPIASSAAAKVEKHLKVPVHNKPVEVLIAKAAVPLEVAA
                     KMSDRQLRIKTIRHLDCVEVGHDRFD"
     gene            769..1755
                     /locus_tag="VG754_14315"
     CDS             769..1755
                     /locus_tag="VG754_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWF20442.1"
                     /translation="MQSEPRNSQRVPAEWSGARSLIEKWLVRRCAAESVINLFGGAVF
                     LVFGIAALIFTSCFTAGIVVVVLMEVNVLLFSIGIKITLFRPTLFIILFLFFTMLSVL
                     HAYKTRWNEDSAARVDFGTALIGLRSLLWEFLSAGPILFILSVQDFSRYVRLSRLDAP
                     QVSALLLWLFDKGGRANFAEITITFPGLNAVRVLPQLRDIPGILWWPDDGEISISDTL
                     KRTFAEILGREPKSSPFFHSYRHERHEPKKPVVEADEGVSQWYAALNLPLFAPLKQVK
                     AQYRKLAKIYHPDAKSSGRANGEIADDEQMKRINEAYHNILKHSQSNAGMAV"
     gene            2286..>3232
                     /locus_tag="VG754_14320"
     CDS             2286..>3232
                     /locus_tag="VG754_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20443.1"
                     /translation="MAMFFACIAGPVFARADVDSLIGKPVIGKNAALELEIFKVDADG
                     QLRYRWQRPSNGDWSSWSSLGGSLLPGIAIATNASGEMVVFAVDRTNHSLMSIHQTAP
                     NSHDWSEWNNLGGEIQPPLALGRNADGRLEVFGTGFGGSNVLHLWQTNPNGGWSGLAS
                     LGGTVEPGLAIATNAIGRLELFGIDAGTKNLMHCRQLERNTTNNWSAWDNLGGSILPG
                     FVAALNAHGRLEVFAVSVTNGAVVRVFQTSADTNAAWSSWMEFGTNITADANLAREPG
                     YVAASRRQSGNSIGADPNLAVASSADGRLEVFAVTSREGA"
BASE COUNT          640 a          847 c          950 g          795 t
ORIGIN      
        1 atcaaaacgg tcatgcccca cttccacgca atcgagatgg cggatggtct tgatgcgcaa
       61 ttggcggtcg ctcattttcg ccgcgacttc cagcggcacg gccgctttcg caatgagcac
      121 ttcgaccggt ttgttatgca ccggcacttt gagatgcttc tcgaccttgg ccgccgccga
      181 tgacgcgatg ggattcaccg ccggtccgcc ccaacgtgac gagccgtccc ccgtttgcgg
      241 ttcagcgatt gatacgccgg gggcgttcgc ttccggaatg cgcaacttgg tgttgcacct
      301 ggggcaattt atctcctcac caacgccgga agcatccact tccagctcca cgccgcactt
      361 ggaacaattg aatacaatat ccataagcgt caggtagttt agggtttaaa actggattgt
      421 agtgcgaatg taactccgtt tcgtccgcgc tgaccagcaa aatttgccgg ccattctcac
      481 tcggcgcatc cgcgcattgc cccggagcgc gaggtcggcg acccgcagca atgtgggaag
      541 gacaggcgcg catgaattag tctggcgtgt cctgtttgtc gcacgttgct gcggctcatc
      601 gagccgcgct ccgtttcgcc cggtcaacgc ggcgggcaat ggcccggcca atcctcccgg
      661 catggccaac aaatcgaatg cagtgcgcgg atgcgccctt ttctcagtgc tgttccttcc
      721 agcgattgac aacgacgctg tttgtcagca acgtggctgc aaggattcat gcaaagcgaa
      781 ccccggaatt cccaacgcgt cccggctgag tggtctggcg cgagatcgct tatcgagaaa
      841 tggctggtgc ggcgttgcgc ggcggaatct gtcatcaacc tgtttggagg cgcggtgttc
      901 ctggtgtttg gaatcgccgc attgatcttt acttcgtgtt tcacggccgg gatcgtggtg
      961 gtggttttga tggaggtaaa tgtgttgctt ttctccatcg gcatcaagat caccctgttc
     1021 cgaccaacgc tgttcataat cctttttctg tttttcacga tgctttccgt tttgcacgcc
     1081 tacaaaacga gatggaacga ggattccgcc gcgagggtgg atttcgggac ggcacttatt
     1141 ggtctccgca gtcttctttg ggaatttctt tccgccgggc cgattttgtt catcctgtcc
     1201 gtccaggatt tctcccgtta tgtgcggttg tcgcggttgg acgctccgca ggtgagcgcg
     1261 ctgctgcttt ggctgtttga caagggcggt cgcgcaaatt ttgcggaaat aaccataact
     1321 tttccggggt tgaatgccgt ccgtgtgctc ccgcaattgc gcgatattcc cggcatcctg
     1381 tggtggcccg atgatggaga aatttccatt tccgacacgc tgaaacggac tttcgctgaa
     1441 attcttggcc gggaaccgaa gtcgtcgccg tttttccaca gctaccgcca cgaacggcac
     1501 gagcccaaga agcccgtcgt tgaagcggat gaaggtgttt ctcaatggta tgcggcattg
     1561 aacctgcctt tgttcgcgcc gctgaaacag gtcaaggcgc agtatcggaa gctggcgaag
     1621 atttatcatc ccgatgcaaa gtcttcgggc cgcgccaatg gagaaattgc ggacgacgaa
     1681 cagatgaagc gcatcaacga ggcgtatcac aacatcctga aacactccca aagcaacgcg
     1741 ggcatggcgg tatgatgggg aattggagtg gtggagttgg ggagtgatgg tgggacaatt
     1801 atttcaaggc catggacgtt tgggaccagg tctttgaaat cgtctgtgtt gcgcgtgagc
     1861 aatatatcag acttggtttg gtccttcgat gcagttgagc cacggattga acactgatga
     1921 aacacggatg ggaggggaga acagccattt tcggcagttc aaaaagcact caccaaatgg
     1981 taactcccct ttcctgccct gcaaaatggc tctgcctgta tatctccgtg ttcaatccgt
     2041 gtttcatctg tggctgaatt gaatggttac gatgggggag agggccgggg tgagggtgag
     2101 cgtaacgact gtggctcccg cgctttcatt gcgaatttgt aacttgtatt tgatttgtat
     2161 tggcaacctc ctcagggcat aattcctgac atacccggaa tgatgtctct ggttgtaaat
     2221 aatatttgct gttaatgttg cctcttcaaa aaagcttcat tcaaatcgcc tggttatggg
     2281 cagtgatggc aatgtttttt gcctgcattg ccgggccggt ttttgcgcgc gctgacgtag
     2341 attccctgat cggcaagccg gtcatcggca aaaacgcggc cctcgaattg gaaattttca
     2401 aagtggatgc cgatggccag ttgcgttatc gctggcagag accgtccaac ggcgattggt
     2461 cgtcatggtc gagtcttggg ggttcattgt tgccgggcat cgccatcgcc acgaatgcgt
     2521 ctggcgaaat ggtcgtgttc gcagtggatc gaacgaatca ttcgttgatg tccatccacc
     2581 agacggcgcc gaacagtcat gattggtcgg agtggaacaa tctcggcggc gaaattcaac
     2641 cgccgttggc cttgggccgc aatgcggatg gccgtttgga agtgtttggc acggggtttg
     2701 gcggcagcaa tgtcttgcat ctctggcaaa ccaacccgaa tggcggctgg tcaggattgg
     2761 cgagccttgg cggcaccgtc gagccggggc ttgccattgc gacgaatgca atcggtcgtc
     2821 tcgaactttt cgggattgat gccggcacaa aaaatctcat gcattgccgg cagcttgagc
     2881 gtaacacgac caacaattgg tcggcatggg acaatcttgg cggctccata ttgccgggtt
     2941 tcgtggcggc attgaatgcg cacggtcgcc tggaagtttt tgcggtaagc gtcaccaacg
     3001 gcgcggttgt tcgcgttttt caaacgtccg cggacacgaa cgcggcgtgg tcgtcctgga
     3061 tggagttcgg cacgaacatc actgcggacg cgaacctggc acgggagccg ggctacgtcg
     3121 ccgcttcccg acggcagtct ggcaatagta ttggcgctga cccgaacctc gcggtggcca
     3181 gcagcgcgga tggacgcctt gaagtttttg cggtcacttc acgcgaaggc gc
//
LOCUS       DASZMM010000594         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_5212200, whole genome shotgun sequence.
ACCESSION   DASZMM010000594 DASZMM010000000
VERSION     DASZMM010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5212200"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VG754_14325"
     CDS             <1..1546
                     /locus_tag="VG754_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWF20444.1"
                     /translation="GHESEVAVERLRPGMRLLIKPGAQFPVDGEIAKGKTESDESNLT
                     GESVPVAKAVGDTVLAGTLNLWGAVEVMVTRAATESALQKIIKLIKEAQQRKAPAQQF
                     TDKFSTYYTYIVLGASLVMFFVWWRVLGLQPFTMTGARQSAFYHTMTLLVVASPCALV
                     LSIPSAVLAAIAWGARHGILFRGGAAVEKLAGINTVALDKTGTLTTGELRVERIESFP
                     CGREAEVAQLAFSLERLSTHPLARAITRYGKQQGLAPIEFDHFESVTGQGLQSERNDK
                     TCFLGRREWLLENAVKLQSFCTEDMAPTQSAIAEPGVCEVWVAEGDLVGRLILRDDIR
                     PQARNVLEELQHAGLRTVVLTGDNKAAADYLKTRLGLDDVRAGLKPEQKVAEIRALGA
                     NGQRVAMVGDGVNDAPSLAAADVGVAMGARGADAALEQAEVVLMHDKLENFLSAFRLS
                     QRARRIIHQNLVISLGTVVVLVCFAMLGKIPLTIGVVGHEGSTVIVVMNSLRLLIGAG
                     EKKTPK"
     gene            complement(1622..1885)
                     /locus_tag="VG754_14330"
     CDS             complement(1622..1885)
                     /locus_tag="VG754_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HWF20445.1"
                     /translation="MATKYQLVLQFQGDTLTDYDAMIKLENLLIKKLGHSAKVDGHDC
                     GSGETNIFIFTFDPALTFLQSQARPGERRKIAISQSCLSGYRM"
     gene            complement(1888..2226)
                     /locus_tag="VG754_14335"
     CDS             complement(1888..2226)
                     /locus_tag="VG754_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20446.1"
                     /translation="MILQKGEKIHVIHRRHLEKEPQRHFVGIVSDYENGIARVTGHVF
                     AVDSIKAVFFRRPELRTRIISLQSGDAIVNILPPSVDLEKIVYKQEKKSVRVTDNDGW
                     HMDISDLAWR"
     gene            2304..3710
                     /locus_tag="VG754_14340"
     CDS             2304..3710
                     /locus_tag="VG754_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWF20447.1"
                     /translation="MNNNNGLNLIAPRLTPVLDPAFRPAVLANRAFHEQARASKNAVP
                     VEIALEQADGSVFRFKTEVLPEGHSQAAGNFIHIERMVKFLLWSRGGFRIYFNGPKRL
                     GEGLQKYYCETPTGKFDSEIIGTRIYDKPIEVVMTRELPAERANTAPLGRHLEGCRIG
                     FDLGGSDRKVAAVIDGKVVFSEETVWDPVKQTDPQYHYNGIMQSLKLAAKHLPRVDAI
                     GGSSAGVYVNNRVKVASLFRGIAPDAFNSRVKDMFLNIRKEWGNVPFEVVNDGEVTAL
                     AGSMSLGQNGVLGIALGTSTAGGYVNMEGNITLWLNELAFMPIDYNPAAPVDEWSGDY
                     GCGVQYFSQQGVGRLLGIAGIETDASLGLPEKLKQVQALMQKGDYRAQKIYHTIGTCL
                     GYAIAHYADFYDFKNVLILGRVTSGPGGDIIIANAKEVLKVEFPELDDCIAFHIPDEK
                     DKRHGQAIAAASLPKLSA"
BASE COUNT          859 a          995 c         1147 g          871 t
ORIGIN      
        1 cggtcacgag agcgaagtgg cggtcgaacg gttgcgtccg ggaatgcgcc tgctcatcaa
       61 gcctggcgcg cagtttcctg tggacggcga aatcgcgaag ggcaaaaccg aaagcgacga
      121 atccaatctc accggagaat cagttccagt cgcaaaagcc gtgggcgata ctgtgctcgc
      181 gggcacattg aatctttggg gcgcggtgga agtgatggtg acacgcgccg caaccgagag
      241 cgcgcttcaa aaaatcatca aactcatcaa ggaagcgcag cagcgaaaag cgcccgcgca
      301 acaattcacc gacaagttca gcacttatta tacttacatc gtcctcggcg cgtcgctggt
      361 gatgtttttt gtttggtggc gggtgttggg cttgcaacca ttcaccatga ccggcgccag
      421 gcaaagcgcg ttttatcaca cgatgacgct gttggtggtg gcttcgccct gcgcgctggt
      481 gctttcaatc ccttcggcgg tgctggcggc gattgcctgg ggcgcgcggc atggaatcct
      541 ttttcgcggc ggcgcggcgg tcgagaaact tgccggcatc aacaccgttg cgctcgacaa
      601 gaccggtacg ctcaccaccg gcgaattgcg cgtggaacgg atcgagagtt ttccgtgcgg
      661 acgcgaggcc gaagtggcgc agcttgcgtt ttctctcgaa cggctttcga cgcatccgct
      721 cgcgcgcgcc atcacgcgct acggcaaaca acagggactt gcgcccattg aatttgacca
      781 ttttgaatcg gtcaccggac agggattgca aagtgagcgg aatgacaaaa cctgctttct
      841 cggccgccgc gaatggttgt tggagaacgc ggtgaaattg caatcctttt gtacggagga
      901 tatggctcca acgcaatccg caatcgccga gcctggggtt tgcgaggtgt gggttgcaga
      961 aggcgatttg gtcggtcgtc tcattttgcg cgacgacatt cgtccacagg cgcgaaacgt
     1021 gctggaggaa ttgcaacacg ccgggttgcg cacggtggtt ttgacgggcg acaacaaagc
     1081 cgcggccgat tatctgaaaa cccggctcgg gttggatgac gtgcgggcgg gattgaagcc
     1141 cgagcaaaaa gtcgcggaga tccgggcttt gggcgcgaat gggcagcgcg tggcgatggt
     1201 cggcgatggc gtgaacgatg cgccgagcct cgcggcggcg gatgtcggcg tggcaatggg
     1261 cgcgcgcggc gcggacgcgg cgttggagca ggcggaggtg gtgttgatgc acgacaaact
     1321 ggaaaatttt ttgtcggcat tccgcctcag tcagcgcgca cggcggatca tccatcaaaa
     1381 tctggttatt tccttgggca ccgtggtggt gctggtttgc tttgcgatgc tcggcaaaat
     1441 tccgctcacc atcggcgtgg tcggccacga aggcagcaca gtgattgtgg tgatgaatag
     1501 cctgcggttg ttgatcgggg cgggagagaa gaagaccccg aagtagacgc gaccgcaaaa
     1561 tcgtttttat tgaatgcgaa attcttccaa agaggcctct ggccaaagcc agttataatg
     1621 ctcacattcg atatcccgat aagcagctct gactgattgc aattttcctt ctctctccag
     1681 gacgtgcttg actttgcaaa aaggtgagtg ccgggtcaaa ggtgaaaata aaaatatttg
     1741 tctctcccga accgcaatcg tgtccatcaa ctttcgctga atggcctaat tttttaatca
     1801 gtagattctc cagcttaatc atcgcatcgt aatctgtgag cgtgtcccct tgaaattgca
     1861 gcacgagctg atatttcgtc gccatagcta acgccacgcc aaatcgctga tgtccatatg
     1921 ccagccgtcg ttgtcggtga cgcgcacgga ttttttctcc tgtttataaa cgattttttc
     1981 aaggtccacc gagggcggca ggatgttcac aatcgcatcg ccggattgaa gcgaaatgat
     2041 gcgcgtgcgc aattccgggc ggcgaaagaa gaccgccttg atggaatcta ccgcgaacac
     2101 atgaccggtc acacgtgcga tgccattttc ataatcgctc acaatcccca caaaatgccg
     2161 ttgcggttcc ttctccagat gccgccggtg gatgacgtga attttttcac ctttttgcaa
     2221 aatcatgccg cagtatccgc aaatgccgcc gggatttcaa cacttgaact tgcccggcat
     2281 ctcccttaaa acgccgtggc gaaatgaaca ataataacgg tctcaatttg attgctccgc
     2341 ggctcacacc tgtcctcgat ccggcgttcc gtccggctgt gttggcgaat cgcgcgttcc
     2401 atgaacaggc gcgcgcgagc aagaacgcgg tgccagtgga gatcgccttg gaacaagccg
     2461 acggctccgt gtttcgtttc aaaacggaag tgttgccgga agggcattcg caagcagcgg
     2521 gaaatttcat ccatatcgag cggatggtga aatttctgtt gtggtcgcgc ggcggttttc
     2581 ggatctattt caacggcccc aagcggctcg gcgaagggtt gcaaaaatat tattgcgaaa
     2641 cgcccaccgg caaattcgat tcggaaatca tcgggacccg catttacgac aagcccatcg
     2701 aggtggtgat gacacgcgaa ttgcctgctg aacgcgcgaa caccgcgccg cttgggcgtc
     2761 acctggaggg ttgccgcatc ggttttgatt tgggcggcag cgatcgcaag gttgccgcgg
     2821 tcattgatgg caaggtggtt ttcagcgagg aaacggtttg ggacccggtc aagcaaaccg
     2881 acccgcagta tcattacaac ggcatcatgc aatcgttgaa attggcggcg aaacatctgc
     2941 cgcgcgtgga cgccatcggc ggaagttctg ccggcgttta tgtgaacaat cgcgtgaagg
     3001 tggcttcgtt gtttcgcggt attgcgcctg atgcgttcaa tagtcgcgtg aaggacatgt
     3061 ttctcaatat tcgcaaggaa tggggcaatg ttccattcga agtggtcaat gacggcgaag
     3121 tgacggcgct tgccggttcg atgtcgcttg gacaaaacgg tgtgctcgga atcgcgcttg
     3181 gaaccagcac tgcgggcggt tacgtgaaca tggaaggcaa catcactttg tggttgaacg
     3241 aactggcgtt catgccgatt gattacaatc ccgccgcgcc ggtggacgaa tggtcgggcg
     3301 attacggctg cggcgtgcaa tatttttcac agcaaggcgt gggacggttg ctcgggatag
     3361 cgggaattga aacggacgcc tcgcttgggc tgccggaaaa gctcaagcag gtgcaggcac
     3421 tgatgcaaaa gggagattac cgcgcgcaaa agatttatca caccatcggc acgtgtctcg
     3481 gctacgccat cgctcattac gcggattttt acgatttcaa aaacgttctc atccttggcc
     3541 gcgtgacgtc cgggccgggc ggcgacatta tcatcgccaa cgcgaaggaa gttttgaagg
     3601 ttgaatttcc cgaacttgac gactgtattg cgtttcacat tcccgatgaa aaggacaagc
     3661 gacacggcca ggcaatcgcc gcggcgagtt tgccgaagtt gtctgcttga tcgcagaacc
     3721 tcgcttgtaa aatttttata acgccctatg atcatggtat gagcgataaa gaagtggtta
     3781 ttgaagctgt ccgccagttg cctgaaaccg tttctttcga ggaaatcagc gagcaaattg
     3841 ccatccttgc tgccatccgc aagggagagg aa
//
LOCUS       DASZMM010000595         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_268590, whole genome shotgun sequence.
ACCESSION   DASZMM010000595 DASZMM010000000
VERSION     DASZMM010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268590"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..1082)
                     /locus_tag="VG754_14345"
     CDS             complement(354..1082)
                     /locus_tag="VG754_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWF20448.1"
                     /translation="MFDLEKQIVEWRRQLAASGIKRREVLDELEAHLREDVAQQIQTG
                     VDAQRAFEAAVRRMGEPRQLKTEFAKASGGTLLWRIKNRLAQLRSHADAMPDIANFTA
                     DGQQTLSFARQEAPLLHHDFIGTEHLLLGLTKSETGIVPNLMQRLGVDSGKVSAEIQK
                     WVGLGQPAPRIPSEIPFTPRAKRALSFAAKEAGALNQTQIGAEHIFLWLLLEGHGVAG
                     LVLKSLGVDAEKARREIRRSLAGD"
     gene            complement(1096..1809)
                     /locus_tag="VG754_14350"
     CDS             complement(1096..1809)
                     /locus_tag="VG754_14350"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HWF20449.1"
                     /translation="MFDLEKAVATWRQEMMRAGIQRPEVLDELESHLLEQAKDLIATG
                     VPEAKAFQKAMEQLGNCTLLKQEFSKVYNEGLISPRNPVLLNLLAFWFALRGFETLLN
                     ARHFFAYTFSNILSFDLSSLKHSASATGWSLFLLLLAIGLYRRQNFWRYCVLLRSALV
                     LMFSFVCYVRNPAALHLPHPVTPGYKVLYEVMCFQVPYDFFCAWLYLPYAVLIVAVYY
                     LSRPHVRALFRPAAKLQNA"
     gene            complement(1802..2134)
                     /locus_tag="VG754_14355"
     CDS             complement(1802..2134)
                     /locus_tag="VG754_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF20450.1"
                     /translation="MITKELVAASAEPLILSLLAKGETYGYAIIQEIKKLSGEKIEWT
                     DGMLYPVLHRLEDKGLVQSRWDQAENGRKRKYYSLKTDGKRALKKQREQWLTVHGTLA
                     KLWEEKYV"
     gene            2323..2997
                     /locus_tag="VG754_14360"
     CDS             2323..2997
                     /locus_tag="VG754_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWF20451.1"
                     /translation="MVRLVLFDIDGTLLHTNAVGIRAFDRTLESEFNVSNGTAGLKFA
                     GRTDTGLVRELFGRHGIEVSKKNFQRFFDNYANWLSQMLAETGGEVCPGAWDLIRELQ
                     ALPNPPALGLLTGNIRLGAEIKLRYFKLWDIFVTGAFADDHEDRNQIAAIAFQRGSQF
                     LGESLRSNQVVVIGDTPHDVACGRAIGAKVLAVATGVSPVAELQSLGPDWAVADLTKI
                     TAREIC"
     gene            complement(3002..3508)
                     /gene="uraD"
                     /locus_tag="VG754_14365"
     CDS             complement(3002..3508)
                     /gene="uraD"
                     /locus_tag="VG754_14365"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HWF20452.1"
                     /translation="MTLSELNSMSQGDFVRIVGPVFENSPWIAEAAWAQRPFADLDHL
                     HRSMMQVVEHAGEEKQVKLIQAHPDLVGRAALAGTLTAESNREQASAGLDQLIAEEIA
                     AFQKNNAAYREKFGFPFVICARLNKKEAILRGFETRLKNPREQEIKTALDEIGKIARL
                     RLQDLITE"
     gene            complement(3505..>3896)
                     /locus_tag="VG754_14370"
     CDS             complement(3505..>3896)
                     /locus_tag="VG754_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWF20453.1"
                     /translation="QLDTAALARMLADAKAASERFAAEGNVKIAWERLWQIEPVLFNN
                     DLIQMCDEAIRETYGKVHRMPSGPLHDAAEVARAGVPTVMMFVQSLHGISHNKIEDTK
                     EEHLELCVAAFDKLAEKAMQWVNGKHP"
BASE COUNT          872 a         1047 c         1008 g          969 t
ORIGIN      
        1 acccgtcgtt taggaggacg ggggggatgg ctggctcctg acaaacgcat tcacgccgac
       61 gggtatgtca gcgaaaaatt tttccaaagt agcccggaat ttgttccgct ttgttttaat
      121 cgccttttaa tggtccggac aaatctacga ttgaagcaag gtgataccaa cgtgtgactc
      181 gaatatccca tttttggctt gctggcacgg gtgtgaagca cacgtcttcg cggagatgat
      241 tctccagttc atcgaggaca tcctgctgtt ggactccctc ggtggccatt tgccgcctcc
      301 aatctgcgat tgcaccttca agattgaatt ggttcatgat gccgttccgg tattcaatct
      361 cccgcaagtg atcggcggat ttctcgacgc gctttttcgg cgtcaacgcc aagacttttc
      421 agaacgaggc cggcgacgcc gtggccttcc agcagcaacc agaggaaaat atgctccgcg
      481 ccgatttgag tttggttcaa agctcccgcc tccttggcgg cgaaggataa agctctcttt
      541 gcgcgtggtg taaatggaat ttccgatgga attctcggcg caggctggcc gagaccgacc
      601 catttttgaa tttcggcaga aaccttgccg ctgtccacac ccaaacgttg catcaggttg
      661 ggaacgatgc cggtttcgga tttggtcaat cccagcaata aatgttcggt gccgatgaaa
      721 tcatgatgaa gcagcggcgc ttcctggcgg gcgaaagaga gggtttgctg gccgtcggcg
      781 gtgaagttgg caatatccgg cattgcatcg gcatgactgc ggagttgcgc cagccgattt
      841 tttattctcc aaagcagcgt tcctccggat gcttttgcga attcggtctt gagctgcctt
      901 ggctcgccca tcctcctgac agcagcttca aaggcccgtt gcgcgtccac tcccgtttgg
      961 atttgttggg ccacgtcctc gcgcagatgc gcttccaact catcaagcac ttcgcgccgt
     1021 ttgatgcccg aagcggccag ttgccgccgc cattcaacga tttgtttttc gaggtcgaac
     1081 ataagatcag gatgttcagg cattttgaag cttggctgct gggcggaaaa gagcccggac
     1141 atgaggcctg ctcaaataat aaacggcaac gatcaaaact gcgtagggca gataaagcca
     1201 agcgcaaaaa aagtcatagg ggacctgaaa acacattact tcatataaca ccttataccc
     1261 aggcgtgaca ggatgcggta aatgtaaagc tgctgggttt ctgacgtaac aaacaaatga
     1321 aaacattaga actagcgctg agcgtaataa cacacaatag cgccagaaat tttgtctgcg
     1381 ataaagccca attgccagca acagtaaaaa caggctccaa cccgtcgctg aagcactgtg
     1441 cttcaaactg gataaatcaa aggagagaat atttgaaaaa gtgtatgcga aaaaatgcct
     1501 cgcatttagg agagtttcaa agccgcgcag tgcaaaccaa aaagccagga gattaagcag
     1561 gactggattc ctgggcgaaa taagcccttc attataaact ttcgagaatt cctgcttcaa
     1621 gagagtgcaa ttccccaatt gctccattgc cttctgaaat gccttcgctt cgggaacacc
     1681 agtagcaatc aaatctttgg cttgttcaag taaatggctt tctagctcat ccagcacttc
     1741 gggacgctgg atgccagcgc gcatcatctc ctggcgccat gtggccacgg ctttttcaag
     1801 atcaaacata tttttcctcc caaagcttcg ccaatgtgcc gtggacggtc agccattgtt
     1861 cacgctgttt tttcaatgcc cttttgccgt cagttttcaa tgaatagtat ttgcgcttgc
     1921 gcccgttctc ggcttggtcc cagcgcgatt ggacgaggcc cttgtcttcg agccgatgca
     1981 acaccggata aagcatcccg tccgtccatt cgattttttc accggaaagt tttttaatct
     2041 cctggatgat ggcgtaaccg taggtctcac ccttggcgag gagcgacagg atgagcggtt
     2101 cggcggacgc ggcaacgagt tccttggtta tcataaataa agtaattctt gaccttgcat
     2161 attgctacta tgcatagcgg tgcaatgtat gtcaacaatt atttttttag ctctcagcga
     2221 acagcgaacc agtctggtgt ccatgcttta gcatgtcatt tgcgtgaaat tttgaacacg
     2281 ctaaagcgtg gacaccaaac aaaacacccg gtactttgtc gcatggttcg tctcgttttg
     2341 tttgatattg acggcacgtt gctgcacacc aacgcggtgg gcatcagggc gtttgacagg
     2401 acgctggaat cggagttcaa cgtctccaac ggcaccgccg ggctcaagtt cgcgggtcgc
     2461 accgataccg ggctggtgcg ggaattattt ggcaggcacg gaatcgaggt gtcgaagaaa
     2521 aattttcaac ggttcttcga taattacgcg aactggctgt cgcaaatgct cgcggaaacc
     2581 ggcggtgaag tttgtcccgg cgcgtgggac ttgatccgcg agttgcaagc tttgcctaat
     2641 ccgcccgcgc ttggtttgct gacgggcaac atccggctcg gcgcggaaat caagctgcgc
     2701 tatttcaagc tctgggacat tttcgtgacg ggcgcgttcg cggacgatca tgaagaccgc
     2761 aaccaaatcg ccgcgattgc gtttcaacgc ggcagtcaat ttctcggcga atcgttgcgg
     2821 agcaaccaag tcgtggtgat cggcgacacg ccgcacgatg tcgcctgcgg gcgggccatt
     2881 ggcgcgaagg tgctcgccgt ggctactggc gtttcaccgg ttgcggaatt gcaatcactt
     2941 ggaccagact gggccgtggc tgatctgacg aaaattactg cgcgggagat ttgctgataa
     3001 tctattcggt aattaaatcc tgcaatcgca gccgcgcgat ttttccaatc tcatcaagcg
     3061 ccgtcttaat ctcctgctcg cgcggatttt tgagacgcgt ctcgaatcct cttaaaatgg
     3121 cttccttttt gttcaatcgg gcgcagatga caaacggaaa cccgaatttc tcccggtatg
     3181 ccgcattatt tttttgaaac gcggcgatct cctccgcgat aagttggtcc aaacccgcgc
     3241 tggcctgctc gcggttcgat tccgcagtca acgttcccgc cagcgccgcg cgtccgacca
     3301 gatcgggatg cgcctggatg agcttcacct gcttttcttc acccgcgtgt tccaccacct
     3361 gcatcatgct tcgatgcagg tggtccaagt ccgcaaatgg tcgttgcgcc catgccgcct
     3421 cggcaatcca tggcgaattt tcgaacaccg gcccgacaat gcgaacgaag tcgccctgcg
     3481 acatcgaatt cagttcggat agtgtcatgg atgtttcccg ttcacccact gcatcgcctt
     3541 ctcggccaat ttatcaaacg ccgccacgca aagctccaga tgttcttcct tggtgtcttc
     3601 gattttgttg tggctgatgc cgtgcaggct ttgcacgaac atcatcacag tcggcacgcc
     3661 ggcgcgcgcg acttcggcgg cgtcgtgcaa cgggcctgac ggcatccgat gaactttgcc
     3721 gtatgtttcg cgaatcgctt cgtcgcacat ctggatcaaa tcattgttaa acaacaccgg
     3781 ctcgatttgc cagagacgct cccacgcgat cttcacattt ccctcggcgg caaatctttc
     3841 gctggccgct ttggcatccg caagcattcg tgcaagcgcg gcagtgtcaa gctggc
//
LOCUS       DASZMM010000596         5715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3826479, whole genome shotgun sequence.
ACCESSION   DASZMM010000596 DASZMM010000000
VERSION     DASZMM010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3826479"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VG754_14375"
     CDS             complement(<1..596)
                     /locus_tag="VG754_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HWF20454.1"
                     /translation="MSHLSVPKSSPEIEAARSIYNIQRWGAKYYDINDAGHVVATPLR
                     DAGAKVDLTDVIEEARGRGLRFPLLIRFQDILRNRVNAINEAFNSAITEFNYQNKYRG
                     VFPIKVNQLREVVEEILDAGKPFNFGLEVGSKPELLAALALQSQSNSLIICNGYKDPT
                     FIRMALMGIKLGKKVILVVEKLEELKQIITVSKQFGVEP"
     gene            815..911
                     /gene="ffs"
                     /locus_tag="VG754_14380"
     ncRNA           815..911
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VG754_14380"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            925..1392
                     /locus_tag="VG754_14385"
     CDS             925..1392
                     /locus_tag="VG754_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HWF20455.1"
                     /translation="MQPPKSKRGPWVKYLVCFCLIIAAAFSALAADPVDDAKAVQGTW
                     APAEAILGGKPMSVAILKTISLKMHSGKYEVLVAGNPDKGTYQIDSASKPTGMTIVGT
                     EGPNRGRKFPAIYEIKGDTLRICYDLSGAKRPAEFKSIPGTRLYLVTYNRKKD"
     gene            complement(1461..2543)
                     /locus_tag="VG754_14390"
     CDS             complement(1461..2543)
                     /locus_tag="VG754_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HWF20456.1"
                     /translation="MKSTLNIPKTGALDFLSLGALVHRLDPGIVPFRKATHCDIYVSG
                     GEFNVSANLSDCFQLKTGIASAMVQYPIGDLIAERVRAMGVKPFYKTFKHDGVHGPNM
                     ATVYSDQGHGVRAPVVFYNRCNEAAAQLKPGDFNWKEIFGTGVRWYHSGGIFAALSHT
                     TAELIVEAMQAAKAAGAVTSFDLNYRAKLWNIAGGNERAVAVLDRIIKNVDVLVGNEE
                     DLQLGLGIPGPEVHAKSKLDPSAFFSMMDNVTKKHPHIKAVATTMREVHSTNRHSWSA
                     VAWMEGDTYQSPVCELDVLDRVGGGDGYAAGFFYGLLTGRSPQEAVNLGWAHGALLAT
                     FPGDTTMATVDQVEAFAKGGSARIQR"
     gene            complement(2616..3341)
                     /locus_tag="VG754_14395"
     CDS             complement(2616..3341)
                     /locus_tag="VG754_14395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF20457.1"
                     /translation="MKISIVVPAFNEQKLIGATLQSIQDAAKSFVSRGWETEIIVCDN
                     NSTDCTSEIARAAGATVVFEPVNQIARARNKGASIATGEWLIFVDADSHPTAELFADV
                     AEQFASGNCLGGGCVIRMDERHFFADRLTSVWNWVSRLRKWSAGSFIFCRTSAFCEVG
                     GFNLELFASEEIDLSNRLKKLARARGEKLVILHKHPMLTSARKMHLYSRKEYFRFFRK
                     AIFSPRATIKSREACMPWYDGRR"
     gene            complement(3338..4051)
                     /gene="ubiE"
                     /locus_tag="VG754_14400"
     CDS             complement(3338..4051)
                     /gene="ubiE"
                     /locus_tag="VG754_14400"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HWF20458.1"
                     /translation="MAKTYYEPGEHRAERVEDLFETIAPQYDRINDLQSFWMHRSWKR
                     RLVRMSAVKAGDRALDVCCGTGDIATALARSGAQTTGLDFSKAMLKVAADRNLKSQIS
                     NLKFLTADAQNLPFGDNSFDVVTIGYGLRNLSSWQRGLEEMHRVAKPGGCLLILEFGK
                     PNNAFWRQLYFGYLKFFVPIFGKLFCGDAATHAYIYESLLAYPAQNGVAAHMRELGCK
                     DVSIVNLIGGAMSINYARK"
     gene            complement(4044..5357)
                     /locus_tag="VG754_14405"
     CDS             complement(4044..5357)
                     /locus_tag="VG754_14405"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009605999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF20459.1"
                     /translation="MNTNGKHVVVLGAGFGGLTFCQKFHHANTHVTIVDRNNHHLFQP
                     LLYQVASAGLSGPDIAQPIRAILSKRCDIDVLMEEVVDINLAERQVVLSKDILTYDYL
                     VLALGSVTSYFGHPEWELYAPGLKSLDDAFRIRRKILLAFEHADDETDPQRQQELMTI
                     VVVGGGPTGVELAGTFAELARFVLRRDFRHIDPSRARIILVEGSPRVLSNFPEHLSDK
                     AAEQLKKLGVEVLTGVRVQNIGERCVQLPDREIRAANIIWAAGVSATPLTNKMAVELD
                     RAGRIKVNPDLSIPGYANVFAIGDMALCMQPDGKPVPGVSPSAMQEARHVARIIEDEL
                     DGKNGKRPAFLYWDKGTMATIGRSAAVAQIKKLQLSGWIAWMMWLVVHLLFLVGFRNK
                     LSVLIQWTYSYLTYKRGARIITGVDDSEHQPPKCEPVQPALAPNG"
BASE COUNT         1369 a         1627 c         1388 g         1331 t
ORIGIN      
        1 ggctcgacac caaattgctt cgacaccgtg ataatctgtt taagttcctc gagcttctcg
       61 accaccagaa tgactttttt gccgagctta atccccatca acgccatgcg aatgaacgtc
      121 gggtccttgt agccgttgca aatgatcagg ctattcgact gactttgcag cgcgagcgcc
      181 gcgagtaact ccggtttgct gccgacttcc aaaccgaaat tgaagggctt gcccgcgtcg
      241 aggatttctt caactacctc acgcagctgg ttcaccttaa tcggaaagac cccgcgatat
      301 ttgttttgat aattgaactc ggtgatcgcg ctattgaacg cttcgttaat cgcgttgacg
      361 cgattgcgca gaatgtcctg gaaacgaatc agcaatggga aacgcagacc gcgtccgcgc
      421 gcctcttcga tgacatcggt cagatcaacc ttcgcgccgg catcgcgcag aggagttgcg
      481 acaacgtggc cggcatcatt gatgtcgtag tacttggcgc cccatctttg gatattataa
      541 atagagcgcg cggcctcgat ttccggcgag gatttgggga cagaaagatg actcatttaa
      601 ttttcacgcg ctaagcgaac ggaactataa gaacgcccgc aaaaactttc aataaggctt
      661 tcaaactttt tcagcattgt agccgtcgag gtgaggaggc ggatttttca aacgtttcaa
      721 cgatttgaaa attaaattta tttcaaattt cgaatttcaa tcgttcgagt ttactcacca
      781 cttgccatcg cgcccgtctc tgttaagttc cagccgccag ggcccatgga atgtggactt
      841 acaaaccccg ccagggcagg aatgcagcaa cggtagtctg ctccgcatcc gccgtggtca
      901 ccctggcatt ttctttttaa ctttatgcaa ccaccaaaat caaaacgtgg cccttgggtg
      961 aaatacctag tctgcttctg cttgattatt gccgcagcat tcagcgccct tgcagccgat
     1021 ccagtcgacg acgccaaagc cgttcaaggc acttgggctc ccgccgaagc catcctcggc
     1081 ggcaaaccca tgtccgtcgc catcctcaaa accatcagcc tcaaaatgca ttcaggaaaa
     1141 tacgaagtcc tcgttgccgg caacccagac aaaggaacat atcaaatcga ctctgccagc
     1201 aaaccgacag gtatgacaat cgttggaacc gaaggcccga accgcggacg aaaattccct
     1261 gccatttatg aaattaaagg cgacaccctg cgcatctgtt acgacctttc cggtgcaaaa
     1321 cgccccgctg aattcaaaag catccccggc actcgacttt atctggtcac ctacaatcga
     1381 aaaaaggact aacacgccct cgttctacct gcacgcaaat tgccgctgca ctaggccttc
     1441 tagcggtaaa gtcaatgcga ctatctttga atccgggcag acccgccctt ggcgaatgct
     1501 tccacttgat cgaccgtggc catcgtcgtg tcgccaggga aagtcgccag caaagctcca
     1561 tgcgcccagc caagattcac cgcctcctgc ggtgaccggc cagtcagcag accgtaaaaa
     1621 aatccggcag cataaccatc tccaccgccg acgcgatcca gcacgtcgag ttcgcacact
     1681 ggcgattgat aggtgtcgcc ttccatccac gccaccgcgc tccaactatg gcgatttgtg
     1741 gaatgcactt cgcgcatcgt tgtcgcgacg gctttgatgt gcggatgttt cttggtcaca
     1801 ttgtccatca tgctgaaaaa cgcgctcgga tcgagcttgc tcttcgcgtg aacttccgga
     1861 ccgggaatgc ctaggccaag ctgaaggtct tcttcgttgc cgacgagcac atcgacattt
     1921 ttgatgatcc ggtcaagcac cgcgacagcg cgctcattgc cgcccgcaat attccaaagt
     1981 ttcgcgcgat agtttaggtc gaacgaagtg acggcacctg cggctttggc cgcttgcatc
     2041 gcttcaacaa tcagttcggc agttgtgtgc gacagcgccg caaaaattcc accgctatga
     2101 taccaacgca caccagtgcc gaaaatctct ttccaattga aatcaccggg cttcagttgc
     2161 gccgccgctt cgttgcaccg gttatagaaa acaactggcg cgcgcacccc gtggccctgg
     2221 tcactataga ccgtcgccat gttcggacca tgcacaccgt cgtgcttgaa cgttttgtag
     2281 aacggcttca cgcccatggc ccgcactcgc tcggcaatta aatcaccaat tggatattgt
     2341 accatcgcgc tcgcaatgcc tgtcttgagt tgaaagcaat cggagagatt cgccgagacg
     2401 ttgaattcac cgccgctgac gtaaatatcg caatgcgttg ctttgcgaaa tggcacgatg
     2461 cccggatcga ggcgatggac gagcgcgccg agggaaagaa agtctaaagc cccggttttc
     2521 ggaatattca aagtcgattt catggtcgtt gtggagttag cagtaacata tcgccgaatc
     2581 gctcggcaat actcgcgaat atgctgacac gctgctcagc gccttccatc gtaccacggc
     2641 atgcacgctt cccggctttt gattgtggcg cgcggactga atatggcttt ccgaaagaac
     2701 cgaaagtatt cctttcgcga atacaaatgc attttgcgcg ccgatgtcag catcgggtgt
     2761 ttgtggagga tcacgagttt ttcaccgcgc gctctcgcca gttttttcaa tcgattgctt
     2821 aggtcgattt cttcagacgc aaacaattcc aggttgaaac cgcccacttc gcagaatgcc
     2881 gacgtccggc aaaagataaa cgaacccgcg ctccatttac gcaatcgact gacccaattc
     2941 cacacgctcg tgagtcggtc cgcgaaaaaa tgccgctcgt ccattcgaat cacgcaacca
     3001 ccaccgaggc agttgccgct cgcaaattgt tcggcgacat cagcaaacaa ttcggctgtc
     3061 ggatgcgagt cggcatcaac aaagatcagc cactcaccgg tcgcgatact cgcgccttta
     3121 ttccgcgcgc gggcgatttg gtttaccggc tcgaatacaa ctgtcgcacc cgcggcacgc
     3181 gcgatttcag aagtgcaatc ggttgaatta ttatcgcaga caataatttc ggtttcccag
     3241 ccacggctga cgaagctttt cgcagcatct tgaatgctct gcaacgtggc cccgatcagc
     3301 ttctgctcgt taaaggcggg gacgacgatc gagatcttca tttgcgcgcg taattgatgc
     3361 tcatcgctcc gccgatcagg ttaacgatgc tcacgtcctt gcaaccgagt tcccgcatat
     3421 gcgcagccac gccattttgg gcagggtagg cgaggagcga ttcataaata tacgcgtgcg
     3481 tcgcggcatc accgcaaaac agcttgccga aaatcggcac aaaaaacttc aggtaaccaa
     3541 aatacagttg ccgccagaac gcgttgttcg gtttgccgaa ttcaagaatg agtaaacaac
     3601 cacccggttt tgcaactcgg tgcatttctt ccaatccacg ttgccatgac gataagttcc
     3661 gcagtccgta gccgatggtg acaacatcaa aactattgtc gccgaaaggg agattttgcg
     3721 cgtcggctgt gagaaatttg agatttgaaa tttgagattt gagattccga tcggcagcaa
     3781 ctttcaacat cgctttgctg aaatccaaac cagtggtctg cgctccactg cgcgcgagtg
     3841 ccgtggcgat gtcgccagtt ccgcaacaca cgtccaatgc gcggtcgcca gcttttactg
     3901 cggacatccg gacaagacgc cgcttccatg accgatgcat ccagaaactt tgcagatcat
     3961 tgatgcgatc gtattgcgga gcgatcgttt caaataaatc ctccacgcgc tcagcgcgat
     4021 gttcgcccgg ttcgtaatag gttttagcca tttggggcga gcgcgggttg caccggttca
     4081 catttcggcg gttgatgctc agaatcgtcc acgcccgtga tgatacgcgc gccgcgtttg
     4141 taagttaaat acgaatatgt ccactggatg agcaccgaaa gcttgttgcg aaaaccgacc
     4201 aggaagagca agtgcaccac caaccacatc atccacgcaa tccagccgga tagctgcagc
     4261 tttttgattt gtgcaaccgc cgccgaacga ccaatcgtcg ccatcgtccc tttgtcccag
     4321 taaagaaatg ctggacgctt tccgtttttt ccatccagtt catcttcgat aatgcgtgcg
     4381 acgtgacgcg cctcttgcat cgcacttggt gacacgccgg gaacaggctt gccgtcaggc
     4441 tgcatgcaaa gcgccatatc gccaatcgcg aacacgtttg catatccggg aatcgagagg
     4501 tctggattga ctttgatgcg tccggctctg tccaattcga ctgccatttt gttcgtcaat
     4561 ggagtcgccg atacaccggc cgcccaaata atattggcag cgcgaatctc tcgatccggc
     4621 aactgcacgc aacgctcgcc gatattctgg acgcgcacac ctgtaagcac ttcaaccccg
     4681 agtttcttga gttgctcggc ggctttatcg gacaagtgtt cgggaaaatt actcagcacc
     4741 cgcggtgagc cttccactaa aatgatccgc gctcgcgatg gatcgatatg ccggaaatcc
     4801 cgacgcaaca caaaacgcgc cagttccgca aacgtcccgg ccagttcaac gccagtcggg
     4861 ccacccccaa caaccacaat cgtcatcaat tcctgttgcc gttgcggatc cgtttcatca
     4921 tcggcgtgct cgaatgccag caaaatcttt cgacgaatcc ggaacgcatc gtccaacgac
     4981 ttgagtcccg gcgcgtacag ttcccattcc gggtgcccga aataactggt cacactgccg
     5041 agtgcgagca cgagatagtc gtaggtcaaa atatctttcg aaagcacgac ctgccgttcg
     5101 gcaagattga tatcgacgac ttcttccatg agcacgtcga tgtcgcagcg cttcgataaa
     5161 atcgcgcgaa ttggctgggc tatatccggt ccggataaac cggccgacgc aacttgataa
     5221 agcaggggct gaaacaaatg gtgattattc cggtcaacga tagtgacgtg agtgttcgca
     5281 tgatgaaact tctggcaaaa tgtcagtccg ccgaaaccgg cgcccagcac cactacgtgt
     5341 ttgccatttg tgttcatctc caatcaattt ttagcggatt gaagtaaaaa gcaaacgcat
     5401 actaaacgca tacggctgta agtcgttgat ggacaggcgt tcattggcta gtcgtgcgac
     5461 gaagcccgat gagtagttca gggtttctca gcggctcaaa aacccttgca gaaaatccac
     5521 aagggtgttt aataattgat gttaaccatt tggatgggcc gtgcccattg ctccgaatag
     5581 tttcggagct tgtttgatgt tagaccaatt catgaaactg cttcaaccgg tgagggcctc
     5641 ttgagtcgcc cttattctcc tcgacctgct cctggtcctt ttacacgcgt caacgggaag
     5701 attcgtgctc gcgaa
//
LOCUS       DASZMM010000597         5385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3564867, whole genome shotgun sequence.
ACCESSION   DASZMM010000597 DASZMM010000000
VERSION     DASZMM010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3564867"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VG754_14410"
     CDS             <1..145
                     /locus_tag="VG754_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HWF20460.1"
                     /translation="AVYHGYGAFCLETQAFPDTINHPSFPSCTLRPGETYRKTTLWRF
                     STQ"
     gene            complement(155..1237)
                     /locus_tag="VG754_14415"
     CDS             complement(155..1237)
                     /locus_tag="VG754_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWF20461.1"
                     /translation="MNYGLNIRPDGALDFVSLGALVHRLDTGIIPFRKASHCNIHVSG
                     GEFNVAANLSDCFRLQTAVTSAMVDYPIGDLIAERVRAMGVKPFYKHFKHDGVNGPNM
                     ATVYSDRGQGVRAPVVFYNRCNEAAAQLKPGDFDWKKIFAGGVRWFHSGGIFAALSST
                     TPELIVEAMQAAKAAGAIVSFDLNYRAKLWNISGGHERAVAVLDRIVKNVDVLVGNEE
                     DLQLGLGIAGPEMAAKSKLDPSAFIGMIGNVVKKFPQVKIVATTLREVHSTNRHSWSA
                     VAWVDGKTHQAPTCELDVYDRVGGGDGFAAGFFYGLLKGVSAEDAVRLGWAHGALLTT
                     FPGDTTMATLDQVEAFAKGGSARIQR"
     gene            1931..2194
                     /locus_tag="VG754_14420"
     CDS             1931..2194
                     /locus_tag="VG754_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20462.1"
                     /translation="MSRLTNVYAGFSFFKNAVIFGGVKKLFFLQEKKMILLPEHSFHA
                     TVCPRLAQSDGRDAHRGCQIVFSRSQVDAKKKSRANSFLADFY"
     gene            2450..3730
                     /locus_tag="VG754_14425"
     CDS             2450..3730
                     /locus_tag="VG754_14425"
                     /inference="COORDINATES: protein motif:HMM:NF025642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HWF20463.1"
                     /translation="MVPWEDAMKFNPYSFPVKSKSKSRRKKEQAANKIDAAIKLALVE
                     LTDYRERRDAFLRLLACVRSRTPLLKPTPGQGNPGWVGPVFLINRLKNLAIRQHHWIR
                     PCETWKPKSGNLRRTFRSLANHLLIEYSVPGFMDSVWDLPAGAEAFRQQSWHIRLGRG
                     AAFRALNVPASLTRKMEHHARQAPDHYSVFQALRHGEIKGIGLHDSCARELVKGRLGQ
                     SMEHPEFWRTVLWFFMAHPETKPEHVNPIIEFIHFNKFAIEEVGTENGTELRTAPFPD
                     FSLKGRRLKSLLRLVSAWQSDLLMDKPGESFSWARSAIQGFRFSEKRPGKEENFDWSI
                     VELLNNTALLAEGCAMRHCVYSYASRCRCGETTIWSLRLRMNGHEKRMVTIEVDPRRR
                     AIIQMRAKCNLRPGGRSREIIDKWASSAGLRFDL"
     gene            complement(3775..>5385)
                     /locus_tag="VG754_14430"
     CDS             complement(3775..>5385)
                     /locus_tag="VG754_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF20464.1"
                     /translation="GPDWWRGDALIAGESRGKIWRTKLVKTADGYVAQNSIIACLKML
                     TIDAIPTPDGGLLVTCHGGAPDWGTGPQGKGKLFKISYTNPAAPQLVFAYAVSPTETR
                     VAFDRPIDPAQFKNLAKQSSITMGRYVTAGERFESFRPGYQAVLDQQKIKRYDLPILS
                     AAIASDNRSIIFQTVPRVTAVNYAIKLPEGSQTERAFNPERHELPQFAAMDVMADLTG
                     VEATWTGAHDKTNTVIWLPHPNLNVSRELTAPSGEHHAFFELLKQKGTLTLRGQLDLD
                     SMLHPKVQPGAKLDYEYPPEKVTVILKSRERLKVKSDARIKHVSSHEYRVTLKPEQKH
                     WLPLEINLPTGGGEPDLEISWFTDEDPRPRAFPLRRILMPFARPETAEPPAIGPRIIP
                     EIAGGDWERGKKVFFSEQAGCFKCHQIAGEGGRIGPDLSNLLYRDYASVYKDITQPNA
                     AINPDHIAYNIQLKDGDIVTGVIMEDTSDKIVLGQATGQNLTIEKSKISGMKASSLSL
                     MPEGLLQALTPQQQRDLFTFLLTTKSEK"
BASE COUNT         1189 a         1449 c         1445 g         1302 t
ORIGIN      
        1 cgcggtctat cacggctacg gcgcgttctg tctggaaaca caggcgtttc ccgacaccat
       61 caatcatcca agttttccct cgtgcacatt gcgtcccggc gaaacttatc gcaagacgac
      121 gctttggcgg ttttcgacgc aatgatttaa tggctcacct ctgaattctg gccgaaccgc
      181 ctttcgcaaa cgcctccacc tggtcgagcg ttgccatggt ggtgtcgccg gggaaggtgg
      241 tcagcaacgc cccgtgcgcc cagccgagtc tcaccgcgtc ctcggcggat acgcccttga
      301 gcaagccgta gaaaaagccc gccgcaaaac cgtcgccgcc accgacacgg tcatagacat
      361 ccagttcgca agtcggtgcc tgatgcgttt ttccgtccac ccacgcaacc gcgctccagc
      421 tatgccggtt ggtcgagtgg acctcgcgca gcgtcgtcgc cacgatcttc acttgcggaa
      481 attttttcac gacgttgccg atcatcccga tgaacgcgct cggatcgagt tttgatttcg
      541 cggccatctc cgggcccgca atgccaaggc cgagctgcaa gtcctcctca ttgccgacca
      601 gcacatcgac attcttcacg atgcggtcca gcaccgcgac agcgcgttcg tgcccgccgg
      661 aaatgttcca aagtttcgcc cggtaattca aatcgaacga aacaattgcg cccgccgcct
      721 tcgccgcctg cattgcctcg acgatcaatt cgggcgtcgt gctcgaaagc gccgcgaaaa
      781 tgccgccgct gtggaaccat ctcacgccgc cggcgaaaat ctttttccaa tcgaaatcgc
      841 ccggcttgag ctgcgccgcc gcttcgttgc agcgattgta gaaaacgacc ggcgcgcgca
      901 cgccttgccc gcggtcgcta tagaccgtcg ccatgttcgg cccattgacg ccgtcatgct
      961 tgaaatgttt gtagaacggt ttcacgccca tcgcgcggac gcgttcggca atgaggtcgc
     1021 cgatgggata atccaccatc gcgctcgtga cggcggtttg cagccggaaa caatccgaca
     1081 agttcgccgc cacgttgaac tcgccgccgc tgacatggat gttacaatgc gacgccttgc
     1141 gaaaaggaat gatgccggtg tccaggcgat gcaccaacgc ccccaacgac acaaaatcca
     1201 acgccccatc gggacggatg ttcagaccat agttcatttt gatttccttc tgatttgagg
     1261 gcaactttgc cccatcatcc aatttgcttc attttttgga ttcgcgccca tgatgcgaaa
     1321 accgtcccgc tttgtcgcca aaattttgtg cgtacgaagg tgaagactgc ttttgccgtg
     1381 ccttgactcg ctcccaaata atggcttgac ttgccttgac tccgattcgg tcgaaaatga
     1441 agtaatacat tggcggagag atggttgccg tgattttcgt atggcttgac caagccttga
     1501 ctgccttgac cgtgtttgat gtttggaggg acgacctgct ggtccttcgt tcagacgtga
     1561 tggaatggcg agtttcactt caaaaaaaat taatttttta cccccgtaat ctgaaaatag
     1621 ctgaaaataa gtgaaaataa ctgaaaatag ctgaaacgat ctgaataaat aagtgaaaca
     1681 aggtctcgaa agcggcgtaa agctgaaacg cgctgtgctt ccatgcctta gcatgttctt
     1741 tccaacacct cgcaccctaa agggtggaca ccgaacaatc ggaatccatc cttccaatac
     1801 tttccctcgc caccactcca aattctcaca ttcaactatc atgcgcccac tttaccatgg
     1861 cattccgtga aaaatcaaac ctaccatagg tttgacacca tttttttgag gtaggtgaaa
     1921 agttcgagtt ttgagccgtt tgaccaatgt ttatgcgggt ttctcgtttt tcaaaaacgc
     1981 ggtgattttt ggcggtgtaa aaaaactttt tttcctacaa gaaaagaaga tgattttgct
     2041 gcctgaacat tccttccatg cgacagtgtg cccgcggttg gcgcaaagcg atggccgtga
     2101 tgctcaccgc ggctgccaaa ttgtgttttc cagaagtcag gtggatgcaa agaagaaatc
     2161 gcgagcaaac agctttttag ccgatttcta ttgaaaaatt caaagttctt gatacgataa
     2221 ttgtgtcatg agcttttgcc attcataatt ttttcgcgaa cggccgctgc tgaaacgggc
     2281 cgtttttctc ttccataacc tccggacagg aacgctccag gaattagcag ggctgtgttg
     2341 ccttctgcca tcgtttccca gcgtttctgt tgatagctgc ttttcgggca attggcccgt
     2401 gagtcgtcat cgctcggagg atcatcattc caaaacccaa gagagcggca tggtgccgtg
     2461 ggaggacgcg atgaaattca atccttatag ctttccagtc aaatcgaaga gcaagagcag
     2521 acgaaagaaa gaacaagcag cgaacaaaat agacgccgcc atcaaattag ccttggtgga
     2581 actgactgac tatagagagc ggcgggacgc tttcctgcgg ttgttggcgt gcgtgcgttc
     2641 gcgcactcca ttgctcaagc ccactcctgg acagggaaat ccgggctggg tcggtcccgt
     2701 atttttgatc aatcgcttga agaatctggc gatccgtcaa caccattgga ttcgcccctg
     2761 cgaaacttgg aaaccgaaga gcggcaatct ccggcggaca tttcgttcgc tcgccaatca
     2821 tttgttgatc gaatattccg tcccaggatt catggattca gtgtgggacc tgccggcggg
     2881 agcggaagcg tttcggcaac aatcgtggca cattcgtctt gggcgaggcg ctgcttttcg
     2941 agctttgaat gtgccggctt ctctcacacg caagatggag catcacgcgc gtcaagcgcc
     3001 tgatcactat tcggtattcc aagccctgcg tcatggtgag atcaagggaa tcgggttgca
     3061 cgactcttgc gcccgcgagc tggtcaaggg ccgtttgggg caaagcatgg aacatccgga
     3121 attctggcgc acagtcctct ggttcttcat ggcgcacccg gaaacaaaac cggagcatgt
     3181 gaatccaatc attgaattta tccacttcaa caaatttgcc atcgaggaag tcggcacgga
     3241 aaacggcaca gaattgagaa cggcaccatt cccggatttt tcattgaagg ggcggagatt
     3301 aaaatccctg ctgcgtttgg tttcggcctg gcagtcggat ctcttgatgg acaaaccggg
     3361 tgaatcgttt tcctgggcga gatctgccat tcaaggtttt cgcttttccg agaagcgtcc
     3421 cggcaaggaa gagaatttcg attggagcat cgttgaactt ttaaacaata ctgcgctgct
     3481 ggcggaaggc tgtgcgatgc gtcactgtgt ttacagttat gcgagccgtt gccggtgcgg
     3541 tgaaacgacg atttggtcgt tgcgactgcg catgaacggt cacgaaaagc gaatggtgac
     3601 gatcgaggtg gatccgcggc ggcgggccat cattcagatg cgggcgaaat gcaacctgcg
     3661 ccccggcgga cgatctcgag aaatcatcga caaatgggca tcatcagcgg gcttgcgatt
     3721 tgatttgtaa acttgatgtg gcagaagcaa ttccgactgc cgccacaacc gtttttactt
     3781 ctctgatttg gtagtcagca aaaacgtgaa taaatcgcgc tgttgctgcg gcgtcaaggc
     3841 ttgcaacaga ccttctggca tcaatgacaa actggacgct ttcatgccgg agattttgct
     3901 tttttcgatg gtgaggtttt gtccggttgc ctggccaagc acgattttat cggacgtgtc
     3961 ttccatgatg acgccagtga cgatgtcgcc gtctttcaac tggatgttgt aggcgatgtg
     4021 gtcgggattg atggcggcgt tcggttgcgt gatgtcctta tagaccgacg cgtaatcgcg
     4081 atagagcagg ttggacaaat ccggcccgat gcgtccgcct tcaccggcaa tttgatggca
     4141 tttgaaacaa ccggcctgct cgctgaaaaa gactttcttt ccacgctccc aatcgccgcc
     4201 ggcaatttcg ggaatgatgc gcggcccaat cgcgggcggt tcggcggttt ccggcctggc
     4261 aaacggcatc aaaatccgtc gcaacggaaa tgcgcgcgga cgcgggtctt catccgtgaa
     4321 ccaggaaatt tcgaggtcag gttcgccgcc gccggtcggc agattgattt ccagcggaag
     4381 ccaatgtttc tgttcgggct tgagagtgac gcgatattcg tgactgctga cgtgcttgat
     4441 acgcgcatcg gatttgacct tgagccgctc gcggcttttc agaatcactg tgaccttttc
     4501 aggaggatat tcataatcga gtttcgcgcc cggttgcact ttcggatgaa gcatcgaatc
     4561 caggtccaat tgcccgcgca acgtcagcgt tcccttttgt ttcagcagtt cgaaaaaagc
     4621 gtgatgctcg ccgctcgggg ccgtcaattc acgcgacaca ttcaaattgg gatgcggcag
     4681 ccaaatgacg gtgttcgttt tatcatgcgc gccggtccat gtggcttcca cgcccgtcaa
     4741 atcggccatc acatccattg cggcgaattg aggcagttcg tgacgttcag gattgaaggc
     4801 acgttcggtc tgagaacctt ccggcaattt gatggcgtag ttcaccgccg ttacgcgcgg
     4861 cacggtctgg aaaatgatcg agcgattgtc cgatgcgatg gcagccgaca aaatcggcag
     4921 gtcgtagcgc ttgatttttt gctggtccaa cacggcttga taccctggac gaaacgattc
     4981 gaagcgttcg cccgccgtga cgtagcgtcc catggtgatg ctcgattgtt tggcgagatt
     5041 tttgaattgc gcgggatcaa tcggcctgtc gaaagccacg cgcgtttccg ttgggctgac
     5101 ggcataggcg aacacaagtt gcggcgcggc gggattcgtg tatgagattt tgaaaagttt
     5161 gcctttgcct tgcgggccgg tgccccaatc cggcgcgccg ccatgacaag tcacgagcaa
     5221 gccgccatcg ggcgtcggga tggcgtcaat cgtgagcatc ttgaggcagg cgatgataga
     5281 attttgcgcg acgtagccgt ccgcggtttt gacgagtttc gtgcgccaga ttttgccgcg
     5341 cgattcgcct gcaatcagcg catcaccgcg ccaccaatcg ggacc
//
LOCUS       DASZMM010000598         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_4941678, whole genome shotgun sequence.
ACCESSION   DASZMM010000598 DASZMM010000000
VERSION     DASZMM010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4941678"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2848
                     /locus_tag="VG754_14435"
     CDS             <1..2848
                     /locus_tag="VG754_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF20465.1"
                     /translation="NLIYSAALRQVNGDPQLAEDVTQCVFTDLARKATQLTCHPSLAA
                     WLYTSARFTAANFRRAEQRRSAREQEAHSMNALTSFPNTESDWRQIRPLLDEAMHTLD
                     KEDREAVLLRHFQNQSFAQVGEQLGLGENAARMRVSRALEKLQQALRKRGVASTTALA
                     FAALLTANGVGAAPAYLAPKIARTALAGTTASAGLSALLSKLFSATTLKATVLVLLLT
                     AITALLVHSNRHHDKTALIITSTNRADDAVQVSGVASITPNIPAAIVATAPANATDGL
                     VLHLEIVSTETGKPVPNVPIDYRCWSQDFHRKQIVSDAQGICDVLYPSNTTELELTTR
                     KDGFADTKLLWNPPHGEVIPASYVLRLDAPVPIGGRVVNVDGDPVAGAKVGWNHEADP
                     TTLRLPQNHEFGWIEVTTDEDGRWRINRIAPEMLPRLYGSARENNHVDSEMVFVSRDR
                     NYEQQLREETLVFQLGRGVALKGVVVDESGQGIAGANVFVGGIASSGRRESKSEADGG
                     FVINGCSSGKEMVTANANGYAVTTIETDISPDAEPVRLLLKPGKTLRLRVLSTTGEPV
                     PSAYIFYDNFGYMTAGAHAVMPQVDFSPETDSEGGAVWTNAPDMELSFNVQAKGYERS
                     GAFKFSPDDEEHVITIRRALVVHGSVVDEASGQPIQHFRVVVGWPQQPDDEPAWSTLE
                     RFWLDFDGGNYRHALEEYPIAGVENRGYYLKFIADNHVPFISRIIKPDEGDVQLDVRL
                     QHAVQTTVTVYKPNGQPAPQADVGLVSSNSRLWLVPGGFNRQNLQSGGSLLQTTADGT
                     FKLPADPNVRRIIAAGPDGYAESTPAALSANPVLQMQSYGSLEADCVSGGHPVAGVDY
                     VLAFEGIPESMVSFDFVTGHVKSDAQGHISVPQLPPGQLSLQRLYSQPDPPGAFSWSS
                     EGAIPFQINSGEKTTIKVEVTNAVPASPVGGTTL"
     gene            complement(3332..3661)
                     /locus_tag="VG754_14440"
     CDS             complement(3332..3661)
                     /locus_tag="VG754_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20466.1"
                     /translation="MLRQIASCAVIAQMHAATLLKKHELTLVDCGHKIPSMSITAVVE
                     DGLIKLPKDVPWSSGTVVRIEPAEQQTPTLLDALKEFDGMADDSPADLVFADVSDFAA
                     RQFLYLL"
     gene            3834..>4073
                     /locus_tag="VG754_14445"
     CDS             3834..>4073
                     /locus_tag="VG754_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20467.1"
                     /translation="MKKLKAMLWVAVCVAALVMSAGKVQAQGRGNVDPAQRRQERIDA
                     LRDKLDVKDDAEWKLIEAAIGKVYDAGREVMGVRMR"
BASE COUNT          970 a         1187 c         1066 g          850 t
ORIGIN      
        1 caacctcatc tattccgccg cactccggca ggtcaacggc gacccgcaac ttgccgagga
       61 cgtgacccaa tgcgtcttca cggacctggc ccgcaaggcc actcaactca cctgtcatcc
      121 gtctttggcg gcatggttgt ataccagcgc tcgtttcacc gccgccaact tccgccgcgc
      181 cgaacaacgc cgttccgcac gcgaacaaga agcccactcg atgaatgccc tcacctcatt
      241 tcccaacacg gaatccgact ggaggcaaat ccgcccattg ctggacgaag ccatgcacac
      301 gttggacaag gaggaccgcg aagccgtgtt gttacgacat tttcaaaacc aatccttcgc
      361 acaggtcggc gaacagcttg gccttggcga aaacgctgcc cgcatgcgcg tttcgcgggc
      421 actggaaaaa cttcagcaag cgctccgcaa acgcggggtt gcttccacca cagcccttgc
      481 cttcgccgca cttttgaccg ccaatggagt gggagctgcg ccagcttatt tggcgcccaa
      541 gattgctcgg acagctctgg ccggaaccac cgcatccgcc ggtctgtccg cgctcttgtc
      601 caaactcttc agtgccacca cactcaaagc gactgtactt gtcctactgc tcacggccat
      661 caccgccttg ctggtccact ccaatcgtca tcacgacaag acagctctga tcatcacgtc
      721 aacgaaccgt gcggacgacg ccgtccaagt atcgggcgtt gcctcgatca cccccaatat
      781 tccggctgcc atcgttgcaa ctgcaccggc aaatgcaacc gatggccttg tgttgcacct
      841 cgaaatcgtt tcgaccgaaa ccggcaaacc ggttccgaac gtgcccatag attaccgctg
      901 ctggagccag gattttcacc gcaaacaaat cgtatccgac gcgcagggca tctgtgatgt
      961 cctttacccg agcaatacca ccgaactcga actcaccacg cgcaaggatg gctttgccga
     1021 taccaaactg ctctggaacc cgccccatgg cgaagtaatc cccgcgagct acgtcctgcg
     1081 ccttgatgcg cccgtaccca tcggagggcg cgtggtcaat gtggatggcg atccggtggc
     1141 cggcgcgaaa gtcggttgga accatgaggc ggacccgacc accctgcggc ttccgcagaa
     1201 ccatgaattc ggctggattg aagtcacgac cgacgaggac ggacgttggc gcatcaaccg
     1261 catcgccccc gagatgctcc ctcgacttta cggcagcgcg agagaaaaca accacgttga
     1321 ctcggaaatg gttttcgtat cgagggacag aaattatgag caacaattgc gtgaagaaac
     1381 acttgtcttc cagcttggcc ggggagttgc cttgaaaggc gtcgtggtgg atgaaagcgg
     1441 ccagggcatc gccggggcga acgtctttgt cggcggaata gccagctcgg gtcgccgcga
     1501 atcaaaatct gaagccgacg gtggctttgt catcaacggt tgctcgtcag gcaaagaaat
     1561 ggtcaccgcc aatgccaacg ggtatgccgt aacaacgatt gagactgata tatcacccga
     1621 tgccgagccg gttcgcctgc tgttgaaacc tggcaagact cttcgtctgc gggtgttgag
     1681 cacgactggc gaaccggttc ccagcgcgta tattttttat gacaactttg gatacatgac
     1741 tgctggagcc catgcagtaa tgccccaagt tgatttttcc ccagagaccg attctgaagg
     1801 cggcgctgtc tggaccaatg ctccggacat ggaattgtcc tttaacgtgc aggccaaggg
     1861 atacgaacgt tcgggagctt tcaaatttag tcccgacgat gaggagcacg tcatcacgat
     1921 taggcgagct ctggtggttc atggttctgt ggtggatgaa gcctccggcc agcccattca
     1981 gcacttccgc gtggtggtgg gttggcctca gcagccagat gatgagccag catggagcac
     2041 attagagcgg ttctggctgg actttgacgg tggcaattac cgtcatgcat tggaagaata
     2101 tccaattgct ggcgtggaga atcgcggcta ctacttgaaa ttcatcgccg acaaccatgt
     2161 gcccttcatt tctcgcatca tcaagccaga tgaaggcgac gtgcaattgg atgtaaggct
     2221 gcaacacgcg gtccaaacca ccgtgacggt ttataagccc aacggccagc ccgccccgca
     2281 ggcggacgtc ggtctggtct cctcaaattc acgactctgg ctcgtcccgg gcggcttcaa
     2341 ccggcaaaat cttcaaagcg gcggttcgct gcttcagacg acggccgacg gcactttcaa
     2401 gctgccagca gatccaaacg tccgccgcat cattgccgcc ggcccggatg gttatgccga
     2461 atccacaccc gccgcgctct ctgctaatcc tgtcctgcaa atgcaatctt acggaagcct
     2521 cgaagccgac tgcgtcagcg gcgggcaccc ggttgcgggc gtggattacg tgctggcatt
     2581 cgaaggcatt ccggaatcaa tggtgtcctt cgatttcgta accggccatg tcaaatcgga
     2641 tgctcaaggg cacatctccg tacctcaact tccacccggt caactttctc tgcaacgtct
     2701 gtacagccaa ccagaccctc ctggcgcatt cagttggtcg agcgagggag cgattccctt
     2761 ccagatcaat tctggcgaaa aaacgaccat caaggttgaa gtaaccaatg ccgttccggc
     2821 cagccccgtc ggtggcacaa ctttgtagga accatgggcc tcagaaatga ttcgcccccg
     2881 tatgaggtga tatatcccag atcatgcggc gtctaccgga gctttgagcg tttcaaacct
     2941 atcatctaca aatatgccag tcctgacgga tttttgagaa atggttgatg attgttagtg
     3001 gctgaattga ctcgcgaaga acgcaaagtt tgaatcacca agcatctgca ggccgggtaa
     3061 aagcgccaag gcgaatccct cgatactcaa ccatcacccc tcaatgattt tacaggtccc
     3121 ttcttctgaa accgcccgtc aacgcaatga tgatacagat caccaaaacc agaccgatac
     3181 cactgccccc gtaaacgggt cctccaaaat aaaacccgcc gccaccgaac aacagtaata
     3241 atatgagaag gattagcaat atgttcacgc aaaaacctag cacaggttcc ccgtgcccgt
     3301 tcaatttccg gtatggagac aattcctccg ctcaaagcag gtataagaac tggcgtgcgg
     3361 cgaaatcgga aacatcggcg aagacgagat cggcgggcga gtcatcagcc atgccatcaa
     3421 attctttcag agcatccaaa agagtcggcg tttgctgttc cgcaggttca atgcgcacaa
     3481 ccgtgccaga actccaagga acatcctttg gaagcttgat caatccgtct tcaacgacag
     3541 cagtgatgct catagaaggg attttatgcc cacaatctac cagcgtcaat tcgtgtttct
     3601 ttaataaagt cgcagcatgc atctgcgcaa taactgcgca gcttgcgatc tgcctgagca
     3661 actcctgcgc agcttcggcg ttctaaatga atgaatgtgg cctgttttgg cgtcgaaaca
     3721 gttattttgg ccattggcac ggtagttgcc ctgtatcaac gacatcaagc taccggaagc
     3781 gcaggttgcg cttgggtaat gtatggaagc aaccaaaaac taatggcaaa aatatgaaaa
     3841 aattaaaagc aatgttgtgg gtggcggtct gtgtggctgc cttggtgatg agcgcgggca
     3901 aagtccaggc gcagggacgc ggcaatgttg acccggccca acgccgtcaa gaacggattg
     3961 acgcgctcag ggacaaattg gacgtgaaag acgatgcgga atggaagttg atcgaagcgg
     4021 ccatcggcaa ggtgtatgac gccgggcgcg aagtcatggg tgtccgcatg cgc
//
LOCUS       DASZMM010000599         6002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_272948, whole genome shotgun sequence.
ACCESSION   DASZMM010000599 DASZMM010000000
VERSION     DASZMM010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272948"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1770
                     /gene="guaA"
                     /locus_tag="VG754_14450"
     CDS             229..1770
                     /gene="guaA"
                     /locus_tag="VG754_14450"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWF20468.1"
                     /translation="MNEQIVILDFGSQYTQVIARRIRECNVYSIILRYDTPASEIAAM
                     QPKGLILSGGPSSVYAKDAPLPDREIFKLGVPILGICFGIQLLAQYLGGKVEKGLKRE
                     YGKGILKVKDSFCPLFANLPETLQVWNSHGDKLTKIPSGFKSVAVSENSDYAAIENRE
                     RKMFGLQFHPEVAHTPQGREIISNFVHNICGCGKNWTMHNYIDQAVEAIRAQVGDEKV
                     ILGLSGGVDSSVAAALLHKAIGDQLTCIFVNNGLLRGREAAVVREVFGRHFKIRLQYE
                     DASKLFLRRLKGVTDPERKRKIIGRTFIEVFEAGTKRAGKARFLAQGTLYPDIIESVP
                     IAGNPAAMIKSHHNVGGLPKKMKFQLVEPLKCLFKDEVRELGLELGLPREIVLRQPFP
                     GPGLAVRILGEVTPRRCDILREADSIVVEEMKASGWYYKIWQSFAVLLPVRSVGVMGD
                     ERTYDYTIALRAVESQDGMTADWVKLPYDLLEKLSTRIINEVKGVNRVCFDISSKPPA
                     TIEWE"
     gene            complement(1824..2171)
                     /locus_tag="VG754_14455"
     CDS             complement(1824..2171)
                     /locus_tag="VG754_14455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWF20469.1"
                     /translation="MMIQRGEIYLVNLSPVAGREQSGHRPVLVVSSDAINRLPLVVTV
                     VVGTKGENIARDYPTNVRLPASETGLPMETVFLCFQIRSLDISRFIGPAVGKLSDVSL
                     QRIENAVRYCLNL"
     gene            complement(2168..2434)
                     /locus_tag="VG754_14460"
     CDS             complement(2168..2434)
                     /locus_tag="VG754_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20470.1"
                     /translation="MPLKNGKYNDMATPAFDKIESEFAQLTPDAQLSLLERLVHRARI
                     SMKHDHDDWDASLSQMAADPQIQRELSQINSEFLAAEGDRLGND"
     gene            complement(2467..2823)
                     /locus_tag="VG754_14465"
     CDS             complement(2467..2823)
                     /locus_tag="VG754_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20471.1"
                     /translation="MQVGQKVVCIDDYFPGPLAKYYINLPVKGRTYTIRAVYLGRGIM
                     HTKPGAADGEIGLLLRELLNGPDPRNVHGQELGFKAERFRPLEETDTTTENEHEDELV
                     SAGTALYSPGKAPDEL"
     gene            complement(2838..4148)
                     /locus_tag="VG754_14470"
     CDS             complement(2838..4148)
                     /locus_tag="VG754_14470"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWF20472.1"
                     /translation="MVPQTVRQSINTSNVLSIIMGGGQGKRLFPLTKERAKPAVPLAG
                     KYRLVDIPISNCINSGLRRIYLLTQFNSASLHRHISQSYKFDHFSGGFVEILAAEQTL
                     SDASWYQGTADAVRKNLIHILNNDFEYLLILSGDQLYRMDFRRIVDQHIETGADLTIA
                     TIPMGRREAESLGIMHIDSQKRITRFVEKPKEPALLDSLRLDRASYEELGIEVDSELF
                     LASMGIYVFNREVLIDMLDNTHTDFGKHIIPNAIKTHRVFSYVYQGYWEDIGTIRSFF
                     EANLDVTSELPRFNFFDMSAPVFSRPRFLPGSKINGAQIDHAVVSDGCIINHARIYQS
                     IIGVRSLVGSGSELRRVIGLGCDFYESMSSIEESERVGRPRIGIGDNTRIENAIIDKN
                     ARIGNNVTISPADKPENVDHPLYFIRDGIVIIPKNGVIPHGMVI"
     gene            complement(4322..4975)
                     /locus_tag="VG754_14475"
     CDS             complement(4322..4975)
                     /locus_tag="VG754_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF20473.1"
                     /translation="MAITVSIVEDDGEARKLLAGWIKRADGLRCVGEHGSAESALTSL
                     PVEKPSVVLMDINLPGMNGVECVRRLKPSLPTTQFVMLTVYEDADHIFNALAAGATGY
                     LLKQTPRQELLAALHDVNHGGSPMASNIARRVVQSFQQTTTTLKSSDELSSREQEVLN
                     LLARGFLYKEIAESLNISVATVNTYIRRIYEKLHVRSRSQAVAKIAHFVDGEPSARA"
     gene            5380..>6002
                     /locus_tag="VG754_14480"
     CDS             5380..>6002
                     /locus_tag="VG754_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20474.1"
                     /translation="MKANRNPFTALMVMLFLVLLTCVARAASDIHFGPTGSETGVNNP
                     DGLWQNDWGPASAGITFDAANPPLTGDTSGSIYVQGNWTGNTGGWDNYNIVVDGNNYY
                     HNASPIFNGGDYTNLEFDIKYDTNSTLAPASAAHLDVGIDHSYGNTVLQTLSFDTASS
                     PLADGNWHHVSIPIAASQTGINNGYGVGFYEWNPGGTMGTMNYWVANV"
BASE COUNT         1377 a         1573 c         1691 g         1361 t
ORIGIN      
        1 ggcacggtga aagtggtgaa cgaataacct gtcaccatcg caggagccgg ggtgagaaga
       61 ctctgtatag gtttcaaatt tgatatttga aattgcaaat caaatgagag ccttgtcacg
      121 tcggctctta cagaagcgcg aagcattggt gtcggcccat aagttgattt tcggttgtca
      181 tttcattttt ctctcgccaa agttgcgttt caaaggcaaa tactttcgat gaacgaacaa
      241 attgtcatcc tcgatttcgg ttcccaatac acgcaagtca ttgcgcgccg gattcgcgag
      301 tgcaacgtct attccatcat cctgcgctac gacacgcccg cgtccgaaat cgcagcgatg
      361 cagccgaagg gtttgatcct ttccggcggg ccgtccagcg tttatgccaa ggacgcgccg
      421 ctgcctgacc gtgaaatttt caaactcggc gtgccgatcc ttggaatctg cttcgggatc
      481 cagttgctcg cgcaatatct cggcggcaaa gttgaaaagg gcctcaagcg cgaatatggc
      541 aagggaattt tgaaggtgaa ggacagtttc tgcccgcttt tcgccaacct tcccgagacg
      601 ttgcaggtct ggaattcgca cggcgacaag ctcacaaaaa ttccgagcgg tttcaaatcg
      661 gtcgccgtca gcgaaaattc cgattacgcg gcgattgaaa atcgcgagcg aaaaatgttc
      721 ggcctgcaat tccatcccga ggtcgcgcac acgccgcagg ggcgcgaaat catctccaat
      781 ttcgtccata acatttgcgg ctgcgggaag aactggacga tgcacaacta cattgaccag
      841 gcggtcgaag caattcgcgc ccaggtcggc gatgaaaagg tgatccttgg cttgagcggc
      901 ggggttgatt cgagcgttgc ggcggcgttg ttgcacaagg ccattggcga ccagctcacc
      961 tgcattttcg tcaataacgg cttgttgcgc ggacgcgagg cggccgtggt gcgggaggtt
     1021 tttgggcggc atttcaagat acggttgcaa tacgaggacg cctcgaaatt attcttgcgc
     1081 cgtctcaagg gcgtgaccga tcccgaacgc aaacggaaaa taatcgggcg cacgttcatc
     1141 gaagttttcg aggctggcac aaaacgcgcg ggcaaggcgc ggtttctggc gcaaggcacg
     1201 ctttatcccg atatcattga atccgtgccc attgccggca atcccgcggc gatgatcaag
     1261 agccatcaca acgttggcgg tttgccgaag aaaatgaagt ttcaactcgt cgagccgctc
     1321 aaatgtttgt tcaaggatga agtgcgcgaa ttgggacttg aacttggatt gccgcgcgaa
     1381 atcgtgttgc gccagccgtt cccgggaccg ggcttggcgg tgcgcattct aggcgaagtc
     1441 acgccacgac gttgcgacat cttgcgcgaa gcggacagca ttgttgttga ggagatgaaa
     1501 gcgagcggct ggtattacaa aatctggcag agcttcgccg tgttgttgcc ggtgcgaagt
     1561 gtcggcgtga tgggcgatga acgcacctac gattatacga ttgccttgcg cgctgttgaa
     1621 tcgcaggacg gaatgaccgc cgattgggtg aagctcccct acgatttgct cgaaaaactt
     1681 tccacgcgca tcatcaacga agtcaaaggc gttaatcgcg tttgtttcga catctccagc
     1741 aagccacccg cgacgattga gtgggagtga aaactacagg tatcagactg gctgcgattc
     1801 ccatttggcc tgaatgcaca gctctataag tttaaacagt atctcaccgc attttcaatt
     1861 cgttggagtg agacatccga aagtttgccg accgccgggc cgatgaagcg gctgatgtcc
     1921 aacgaacgga tttggaaaca aagaaaaact gtttccatcg gcaagccggt ttcagatgca
     1981 ggaaggcgca cattcgttgg ataatcgcgc gctatatttt cgccttttgt gccaacgaca
     2041 actgtgacga ccagaggcaa tcggttgatg gcatcactcg aaacaaccaa aacggggcgg
     2101 tgtcctgact gttcgcgtcc ggcaacaggg ctcaaattga ccaggtaaat ttctccgcgt
     2161 tggatcatca atcgtttccc aaccggtccc cttccgctgc cagaaactcc gaattgatct
     2221 ggctgagttc ccgctggatt tgaggatcag ccgccatctg cgatagtgaa gcatcccaat
     2281 cgtcgtgatc gtgcttcatt gatatgcgag cgcgatgtac aagccgttct aacaggctga
     2341 gttgggcatc tggcgtgagt tgcgcgaatt cgctctcaat tttgtcgaac gctggagttg
     2401 ccatatcgtt atattttccg tttttgagtg gcaagtcaac ctgcttcggt ttgcaagaaa
     2461 atgcgcttaa agctcatccg gcgctttgcc gggcgaataa agcgcagttc cggcggacac
     2521 caactcgtct tcatgctcgt tttccgtggt ggtgtcggtt tcttcgagcg gacggaaacg
     2581 ttcggccttg aagccgagtt cctggccgtg gacgtttcgc gggtcgggcc cattcagcaa
     2641 ttcccggagc agcaatccga tttcgccatc tgccgcgccc ggcttggtgt gcatgatgcc
     2701 gcgcccaaga tatacggcgc ggatggtgta agtgcggcct ttcactggaa gattgatgta
     2761 atacttcgcc aatgggccgg gaaaataatc gtcaatacaa acaacttttt gtccaacttg
     2821 cataaaaaaa ttcgcattta aatcaccatt ccatgcggaa tcacgccgtt tttgggaatg
     2881 atgacgatgc cgtcgcggat gaagtagagg ggatggtcca cgttttccgg cttgtcggcg
     2941 ggcgaaatgg tgacgttgtt tccgatgcgg gcattcttgt caatgatggc gttctcgatg
     3001 cgcgtgttgt cgccgatgcc gatgcgaggg cggcccaccc gttcgctttc ctcgatggaa
     3061 ctcatcgatt cataaaaatc gcaacccaaa ccgatgacgc gccgcaattc gctgcccgac
     3121 ccgaccaggc tgcgcacgcc gatgatgctt tgataaattc gcgcgtggtt gatgatgcag
     3181 ccgtcggaaa caaccgcgtg atcaatttgc gcgccgttga tctttgagcc tggcagaaag
     3241 cgcggacgcg aaaacaccgg cgcgctcatg tcaaaaaaat tgaaacgtgg caattccgaa
     3301 gtgacgtcca ggttcgcctc aaaaaacgag cggatggtac cgatgtcctc ccaatagcct
     3361 tggtacacat acgagaaaac gcgatgggtc ttgatggcgt tcgggatgat gtgcttgcca
     3421 aaatcggtgt gcgtgttgtc gagcatgtcg atcaagactt cgcggttgaa aacatagatg
     3481 cccatcgaag ccaggaacaa ttcgctgtcc acctcgatgc ccagttcctc gtaagaagcg
     3541 cggtcaaggc gcaacgagtc caacaacgcg ggctccttcg gtttttccac gaagcgggtg
     3601 atgcgctttt gcgaatcaat gtgcatgatg ccgagcgatt cggcctcgcg gcggcccatc
     3661 ggaatggtgg cgatggtgag gtccgcgccg gtctcgatgt gttggtcaac gatgcggcga
     3721 aaatccatgc gatacaattg atcgccgctc aggatgagca ggtattcaaa atcattgttg
     3781 aggatgtgga tcaaattttt gcggacggca tcggcggtgc cctgatacca ggaagcgtcg
     3841 gaaagggtct gctcggcggc aaggatttcg acgaacccgc cgctgaaatg gtcgaacttg
     3901 tacgattgcg agatgtggcg atgcagcgaa gcggagttga actgggtcaa cagataaatg
     3961 cggcgcaggc cggaattgat gcaattggag atggggatgt ccacgaggcg atatttgccg
     4021 gcaagaggca cggcgggctt ggcgcgttcc ttggtcagcg gaaacagccg tttgccctgg
     4081 ccgccgccca tgatgatgga caatacgttg ctggtgttga tggactgccg cacagtctgt
     4141 ggaaccataa aaagcccttt cgtaccaagt gttctacctt tttataaccc gtaacaggcg
     4201 gcaagggcaa gtgacattga acgatccgca cgcagattac gaggcaaacg tgtcgttcaa
     4261 tgcgaagcga gcggaaaaag cgtgagatgc cttaaaaagc gtgaataaaa gagggccgtg
     4321 ctcacgcccg cgcggacggt tcgccatcga cgaagtgggc gatcttggcg acagcttgcg
     4381 agcgcgaacg gacgtgcagc ttttcgtata tcctgcggat gtacgtgttc acggtcgcca
     4441 cgctgatgtt caacgattcg gcgatctcct tgtataaaaa tccgcgcgcg agcagattga
     4501 ggacttcctg ttcgcgggag gacaattcat cggagctttt gagcgtggtg gtcgtctgtt
     4561 gaaaggactg cacgacacgg cgggcgatgt tgctggccat cggcgaacca ccgtgattga
     4621 cgtcgtgcag ggcggccaaa agttcctgcc gcggcgtttg tttgagaaga taacccgttg
     4681 ccccggcggc aagcgcgttg aagatgtggt cggcatcttc gtaaaccgtg agcatcacga
     4741 actgtgtcgt cggcaatgac ggtttcaatc gccgcacgca ttccacgccg ttcatgccgg
     4801 gaaggttgat gtccatcaac accacgctcg gtttttctac cggcagggaa gtcagcgcgc
     4861 tctcggccga gccgtgttcg ccgacgcatc gcaagccgtc cgcgcgcttg atccaaccag
     4921 ccaggagttt gcgcgcctcg ccgtcgtcct caacaattga aactgtaatg gccacgaatc
     4981 agactatgta cgagtctttc acacattggg cgtcatcaat tttaatgaca agactattct
     5041 cgaattctca ccgaacggaa agtcatctcc tgctcaacac ggcaaacatc tccaaaaagc
     5101 ttgcaaatgc aggtgattct ccgcgtgttt cgcgatgcga accgaagcca ttgcgttcgc
     5161 gcaaggcgga tttgtgatgc cggtttgact tgtcatcagt tttaacgaca tgcgctgaaa
     5221 cggcaatccg gttagtcttg ccacagatac cccggtaaaa gtcctcggat gagggcgcgg
     5281 ccctggcccg ctgtttgcct gccgcccggc gaagatacga agtgcgcgaa cgaaccatca
     5341 accaacaatg ccgcatcgtc aaccaacctg ccaatcaata tgaaagccaa ccgaaatcca
     5401 ttcacagcgc tcatggtaat gcttttcctg gtgctgctaa cctgcgtggc ccgcgccgcg
     5461 tctgacatcc atttcggccc gaccggcagc gaaacgggcg tcaacaaccc cgacggcttg
     5521 tggcaaaacg actggggtcc agcctctgcg ggcatcacct ttgatgccgc caaccctcct
     5581 ttgaccgggg acacatccgg ttccatttat gtgcagggca attggaccgg caacaccggc
     5641 ggttgggaca attacaatat cgtggtggac ggcaacaatt attatcacaa tgccagcccg
     5701 atctttaacg ggggcgatta cacgaacctc gaattcgaca tcaaatatga cacgaattcc
     5761 acccttgccc cggcgtccgc cgcgcatctg gacgtgggca ttgatcattc ttatgggaac
     5821 acggtgttgc agactttgtc ctttgacaca gcttcgtcgc cgcttgcgga tggcaactgg
     5881 catcatgtga gcattccgat cgccgccagt caaaccggca tcaataatgg ctacggggtc
     5941 ggcttctatg aatggaatcc aggcgggaca atgggcacca tgaattattg ggtggcaaac
     6001 gt
//
LOCUS       DASZMM010000600         3860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_274319, whole genome shotgun sequence.
ACCESSION   DASZMM010000600 DASZMM010000000
VERSION     DASZMM010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274319"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VG754_14485"
     CDS             complement(<1..236)
                     /locus_tag="VG754_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20475.1"
                     /translation="MAEIAVEDLKGLRLLKSFNKTLRRVCRRKRHQRVGADAHHRRQR
                     AEYLSLYLFGLLNPVVRSMRALCELGRTQRAKECL"
     gene            458..1600
                     /locus_tag="VG754_14490"
     CDS             458..1600
                     /locus_tag="VG754_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HWF20476.1"
                     /translation="MASNKPVRAWLLAFVALGFMNLSSQAQIQSTNNQSTTQSTNDMG
                     IRWLEKAGPVPEFKIPASRRAWESKRKKVRAELNQLLGKLPPRPKVPAVQTLSREDRG
                     DYIVEKFQFDNGAGSIVPGYLVLPKNHPGKAPAILYCHWHGGLYDSGKEELFHASHTP
                     FEAGPLFAKRGFVVIAVDSYCFGERNGQGPGGPDEKGSPGEMTAAKFNLWVGRSLWGM
                     ILRDDLMALDYLVSRPEVDTSRIGVTGMSMGATRAWWLMALDDRIKTGVAVACLTRYQ
                     NLIQHEELKAHGIYYFVPGLLNHFDTEAVVALIAPRPALFMNGGDDPGSPTDGIHAIE
                     SVVRPAYRLYGKESDFESIIYPGQGHVYTPEMWEKTLTWFDGHLKQ"
     gene            complement(1635..2033)
                     /locus_tag="VG754_14495"
     CDS             complement(1635..2033)
                     /locus_tag="VG754_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20477.1"
                     /translation="MKTKSSNFWIFCIGAILVVICCRLIFLGTILADTTEYYDSKPLK
                     DKSLPDWAGTNAIPLSPEKAVRKAIDYANSQKAHPVNWDVDAVELRRYSSDSAWFYTI
                     TLTDRKSGAYEFKVVRVLLNGEIWKPRNSK"
     gene            complement(2289..3236)
                     /gene="xerA"
                     /locus_tag="VG754_14500"
     CDS             complement(2289..3236)
                     /gene="xerA"
                     /locus_tag="VG754_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HWF20478.1"
                     /translation="MENLVEDFLQFLRHERGQSEHTQKTYSALLHRFVTWARKQNLND
                     WKSVELPHLMAFLTHERQRPLADEKNSARKLSSESVYLEIAALRAFYRFAETEKLLPV
                     NVAEHLSLPRRWKRLPKSLTDAEIKQLLKPADKSMPQTPQSLCDRAILELAYASGLRL
                     AELRGVRLEQLHLEAGFINVIGKGNKERVVPVGKKAVEAIEIYLRQGGRPELVTPRSP
                     ANLFLTRRGTPFAAVTMWKRIRDYAKRAGITRKITPHMLRHSFATHLLEHGADLRVIQ
                     ELLGHASISTTEIYTHIAGNRLREIHQKFHPRSELEKKK"
     gene            complement(3348..3677)
                     /locus_tag="VG754_14505"
     CDS             complement(3348..3677)
                     /locus_tag="VG754_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20479.1"
                     /translation="MSKRACYIASVLFAIGALFVNSTRYQMATEASRQKGKELQSMMQ
                     HGATKEYSFEAKRLSRQTQLLYYAWFILLTCSIVFLIAAVIRGEPGYYLILILLICFA
                     AMAGMIA"
     gene            complement(3678..>3860)
                     /locus_tag="VG754_14510"
     CDS             complement(3678..>3860)
                     /locus_tag="VG754_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20480.1"
                     /translation="ILTNISDVVVHSDQFIQGLKRFWLFRHLFTAHGQPKQPKSKAPP
                     TQQRPGEHLVSPQQRQ"
BASE COUNT          866 a         1047 c         1004 g          943 t
ORIGIN      
        1 aggcactcct tcgccctttg cgtccggccc agttcgcaca gcgcgcgcat gctgcgcacc
       61 accggattga gcaacccaaa aagatagaga cttaaatact cggctcgctg acgccgatgg
      121 tgggcatccg ccccgacgcg ctggtgccgt ttgcgccggc aaacccgccg cagagttttg
      181 ttaaaactct tgagcaggcg caaacccttc agatcttcca ccgcaatctc ggccacgcat
      241 tgattaaagc gcggcttcca gcgttgtaca agttacaatc ccgtgacagg cctgttacat
      301 gactgtgcca tccaccacaa tattaccgag catccattgt gccggacact attggtcacc
      361 gacccgcgct ccgggcaatg cgcggatgcg cgtcgatgtt tcaggcaatc tcgtttctcg
      421 cttgcctaat gcgcctgtct ccgctgatgc tctctgaatg gcgtcaaaca aaccggttcg
      481 cgcgtggttg ctcgctttcg tggcgcttgg ttttatgaat ctttcaagcc aggcccaaat
      541 ccaatccacc aataatcaat ccacgactca atccaccaat gacatgggga tacgctggct
      601 ggaaaaagcc gggccggtgc cggaatttaa aattcccgcg tcgcgtcgcg cctgggaatc
      661 caaacgcaaa aaagtccggg cggaactgaa ccagcttctc ggcaaacttc cgccgcgccc
      721 aaaagtgccc gccgtgcaaa ccttgtcgcg tgaagatcgc ggcgattaca tcgtggaaaa
      781 atttcagttc gacaacggcg cgggctcaat cgtgcccggt tatctcgtgc tgccaaaaaa
      841 tcatccaggc aaagcgcccg caatccttta ttgccactgg catggcggtc tttacgacag
      901 cggcaaggag gaattgtttc acgcgagcca cacgccgttt gaggccggcc ccctatttgc
      961 aaagcgcggt ttcgtggtga ttgcggttga ttcttattgt ttcggcgagc gcaacgggca
     1021 gggtcccggc ggcccggacg aaaagggaag tcccggcgaa atgaccgcgg ccaagtttaa
     1081 tttgtgggtc ggacgatcgc tttggggaat gatcttgcgc gacgatttga tggcgctgga
     1141 ttatctggtg tcgcggccgg aagtcgatac ctcgcgaatc ggcgtcaccg gcatgagcat
     1201 gggcgcgacg cgcgcgtggt ggttgatggc gctcgatgac cgcatcaaga ccggcgtggc
     1261 ggtggcttgc ctgacgcgct atcaaaatct catccaacac gaagaattga aggcgcacgg
     1321 gatttattat ttcgttcccg gtttgctgaa tcattttgat accgaagcgg tggtggcatt
     1381 gattgcgccg cgccccgcgt tgttcatgaa cggcggggat gatccgggtt cgccgaccga
     1441 cggcatccat gcgattgaat ccgtggtgcg tcccgcttac cggctttacg gcaaggaatc
     1501 ggatttcgaa agcatcattt accccgggca gggacatgtt tacacgccgg agatgtggga
     1561 aaagaccctg acgtggttcg acggccactt gaagcagtaa ttacttccca tctgtgcgct
     1621 atatctagct tggatcactt gctgtttctc ggtttccaaa tctcaccatt caacaaaacg
     1681 cgcaccacct tgaattcgta tgctccggat tttcgatctg taagcgttat cgtgtagaac
     1741 catgcagaat cggaagagta tcgtcgcaat tcaactgcat caacgtccca atttaccgga
     1801 tgggcttttt gagaattggc gtaatcaatt gccttacgca ccgccttttc agggctcaac
     1861 ggaatggcgt tagtgccggc ccaatcagga agtgatttat ctttaagcgg cttcgaatcg
     1921 taatattccg tggtatccgc aaggattgtt cccaaaaaaa tgagccgaca acatatcaca
     1981 accaaaatag ccccgatgca gaagatccaa aaattggagg atttagtttt cattggttcc
     2041 atgtttttta tgctttaaaa cccttgaagt tgcaagcggg ctagtactta cccctttgca
     2101 ttgcgctgcg cgcccccact gaccgtaatt gtcagtcgcg ctcccacttc gcttattcta
     2161 ccaacctctt cgaggtttta tgattccaga ctgttcgatg cattttttat ccttcacctt
     2221 gaatctcacg taacacttct ctctgttcgt gtcttggtgg ttaattcctg ctttcctgaa
     2281 ttcctaattt attttttctt ttccaactcc gagcgcgggt gaaatttttg atggatttcc
     2341 cgcaaccggt tgccggcgat atgcgtgtaa atctccgtcg tgctgatgct ggcgtggccg
     2401 aggagttcct ggatgacgcg caagtccgcg ccgtgctcaa ggagatgcgt cgcaaaactg
     2461 tgccgcaaca tgtgcggcgt gatcttgcgg gtgatgcccg cgcgcttggc gtaatcacgt
     2521 atccgtttcc acatggtgac cgccgcgaat ggtgtgcccc gccgcgtcaa aaacagattg
     2581 gcgggcgaac gcggcgtcac taactcgggt cgaccaccct gtcgaaggta aatttcaatg
     2641 gcctcgactg cctttttgcc gaccggcacg acgcgttcct tgttgccctt gccgatgacg
     2701 ttgatgaaac cagcttcgag gtgcagttgt tcgagccgca cgccgcgcag ttcggcgagc
     2761 cgcaggccgg aggcataagc cagttccaaa atcgcgcggt cgcaaaggga ttgcggcgtc
     2821 tgcggcatgg atttgtcggc aggtttcagc aactgtttga tttcggcatc ggtcaacgat
     2881 tttggcaatc gcttccagcg gcgcggcagc gaaagatgtt cggcgacatt gacgggaagc
     2941 aatttctcgg tctcggcgaa gcgatagaac gcgcgcaacg cggcgatttc caggtaaaca
     3001 ctttccgaac tcaatttgcg cgcgctgttt ttttcgtcgg cgagcggacg ctggcgttca
     3061 tgcgtcagga acgccatgag gtgcggcagt tccactgatt tccagtcgtt gagattttgt
     3121 ttgcgcgccc aggtgacgaa ccgatggagc aacgcggagt aagttttttg cgtgtgttcc
     3181 gattgcccgc gttcgtgccg caggaattgc aaaaaatctt cgaccaaatt ttccacgcga
     3241 ggattttaaa gctccaagca ccaacaacca agctccaatg aagctccaat gaagctccaa
     3301 tgaagctcca aacaccaata cccaagatcc agagaacaac ttatccttca agcaatcatt
     3361 cccgccatcg cggcgaaaca aatcaacaga ataaggatta aatagtatcc cggctctcct
     3421 cgaataactg ccgcgattaa aaatacaatg ctgcacgtca acagaataaa ccatgcataa
     3481 tataacaact gtgtttggcg cgacaaacgt ttcgcttcga aagaatattc tttagttgct
     3541 ccatgttgca tcatagattg aagttctttg cccttctgcc gcgatgcttc cgttgccatc
     3601 tgataccgtg tcgaattcac aaacaatgca cctattgcaa acaggactga agcgatgtaa
     3661 caagcccgtt tactcattta ttgcctttgt tgcggtgaaa ccaaatgttc gccggggcgc
     3721 tgttgcgtgg gcggcgcttt ggatttcggc tgtttgggtt ggccgtgcgc ggtgaaaaga
     3781 tggcggaaaa gccagaaccg tttcaatccc tggatgaact ggtcggaatg caccaccacg
     3841 tccgaaatgt tggtcaaaat
//
LOCUS       DASZMM010000601         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2744305, whole genome shotgun sequence.
ACCESSION   DASZMM010000601 DASZMM010000000
VERSION     DASZMM010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744305"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VG754_14515"
     CDS             complement(<1..225)
                     /locus_tag="VG754_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HWF20481.1"
                     /translation="MLIKILIGLAIVVIAFVAIVAMQPSEGRISRSMTMPVPASAVFA
                     QVNDFHNWQAWSPWAKRDPAAKNSFEGASSG"
     gene            complement(245..736)
                     /locus_tag="VG754_14520"
     CDS             complement(245..736)
                     /locus_tag="VG754_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWF20482.1"
                     /translation="MPAQTNQPIEKQIPKELVVVRTFQAPRALVFKAWTDPKQVAQWW
                     GPRGFTNPVCEWDARPGGLIHVEMRGPDGISHPMKGVFREVVKPERLVFTVTAFEDEH
                     GNPRLENINTVTFTEMNGVTEQTLHVVVLKSGPEIAQALAGMNAGWNQSLDKLMEFVA
                     KLK"
     gene            complement(748..1287)
                     /locus_tag="VG754_14525"
     CDS             complement(748..1287)
                     /locus_tag="VG754_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWF20483.1"
                     /translation="MTARKNNPAESTVNTGMEPFIISRTFDAPRELVFKAWTERERLM
                     QWFGPKGFAMTSARLDLRPGGNFHYCLRSPDGKEMWGKFVYREIVAPERIVWVNSFSD
                     KDGNMARHPMMPNWPAEMLSTAKFTENAGKTTVTINWLPLNPTVEERNVFETMRSRMN
                     EGWTGTFEQLEKYLTETKS"
     gene            complement(1259..1633)
                     /locus_tag="VG754_14530"
     CDS             complement(1259..1633)
                     /locus_tag="VG754_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWF20484.1"
                     /translation="MTTDHLSVTFAALADPTRRAILSRLTSGEVSVKELARPFDMSLP
                     AVSKHLRVLERSGLIVRSRAAQWRPCRLKAKPLKDAVDWLEHYRRFWEESLDRLDDYL
                     QELQAKTKQHKTQNDRKKKQSR"
     gene            1892..3598
                     /gene="sulP"
                     /locus_tag="VG754_14535"
     CDS             1892..3598
                     /gene="sulP"
                     /locus_tag="VG754_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HWF20485.1"
                     /translation="MKMAWSFRPRLADTLKNYSREQFMSDAIAGFTVGIVALPLAMAF
                     GIASGVTPQAGIFTAVVAGFIISALGGSRVQIGGPTGAFVSIVLATIVTYGLDNLYIC
                     TVLAGLMLLVMGFCKMGNLIKFIPFPVTMGFTCGIAVLIFSTQIKDFLGLQVEHVPGD
                     FVGKLQMLYENIGAIDWPTVVLAVASIIVIKTWPAKLAKRVPGSIVALLLGTVVVTVC
                     HLKVATIGSVFGGIPTGLPKIHLPPLSWEKIQHLIEPAFTIALLAAIESLLSAVVADG
                     MIEDRHDSNQELIAQGLANIASPFFGGIPATGAIARTATNVRCGGRSPIAGITHAVTL
                     LIIILVAAPLAKYIPLAVLSAVLVVVAYNMGEWHQFRRLRKWPRSDSAVFLVVFALTV
                     FTDLPTAVKIGMVLAAALFVNRISQTMQITAVDQSTSAESAHNSVIGKEVPKGVMVYR
                     VFGAFLFGAADKLETALKRAHQEPEILILKMQSVLAMDATGLNALEDIYEKLHRKGRH
                     LILSGPHTQPLVTMQRDGFIDQLGAENVCPHLDAALERAREILDAKAAKNVNVIPKPA
                     TV"
     gene            complement(3738..>4821)
                     /locus_tag="VG754_14540"
     CDS             complement(3738..>4821)
                     /locus_tag="VG754_14540"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWF20486.1"
                     /translation="QRLAVTALGGSPATPPEGLTAEIVLFKTYDELLAAPAGSLKGKI
                     AVVTQPMVRTQDASGYSEANKIRSLGPAEAAKRGAVGYLLRSLATDSRRLPHTGNTHF
                     PIDGPRIPAAALSVPDAEQLDRLVALGEPVKVKLVLTPRDLGTVASQNVIAELKGREK
                     PDEIVLLGAHLDSWDLGTGAIDDGAGVAIVMAATKLIHDLPQRPRRTIRVVLFGSEEM
                     GLLGGRAYAASHHAELSKYIIVAEPDLGQGPIYRFETGVANPDEPTLKTIRAALAPLG
                     ILPGGNQSRGSSDVEPLAEAHVPAVTLEMDATDYFDLHHTADDTVDKIKPERLNQSTA
                     AFAVFTYLAAELDGNYRAHESNGSGK"
BASE COUNT          972 a         1298 c         1397 g         1154 t
ORIGIN      
        1 gccggacgac gcgccttcaa acgaattttt ggccgcagga tcgcgttttg cccagggcga
       61 ccatgcctgc caattgtgaa aatcgttcac ctgggcgaac accgcgctcg ccggcacagg
      121 catggtcatt gatcgcgaaa tccggccttc tgatggctgc atggcaacga tggcaacgaa
      181 cgcgatcact acgatggcca atccaatcaa tatttttatc agcatagctt tgagtagttg
      241 agttttattt aagtttggcg acaaattcca tcagtttatc gaggctctgg ttccaaccag
      301 cattcattcc ggccaacgcc tgtgcgattt ccggtcctga cttcagcacg accacgtgca
      361 atgtctgctc cgtcacgccg ttcatttcag tgaaggtcac ggtgttgata ttttcgagcc
      421 ggggatttcc gtgttcgtct tcaaaagcgg tgacggtgaa caccagccgc tcgggtttga
      481 cgacttctcg aaaaacgcct ttcatcggat gcgagatgcc atcggggccg cgcatttcga
      541 cgtgaatgag accgccgggc cgggcgtccc attcacagac cgggttggta aaaccgcgcg
      601 ggccccacca ttgcgcgact tgtttcgggt ccgtccacgc cttgaacaca agcgcgcgcg
      661 gcgcttgaaa cgtgcgcacg acgacaagtt ccttgggaat ctgtttttcg attggttgat
      721 ttgtttgagc aggcatattt tttcctttca cgattttgtt tcagtcaggt atttctccaa
      781 ttgttcgaat gttccggtcc agccctcgtt cattctcgag cgcatggtct caaagacgtt
      841 gcgttcctcg acagtgggat tgagcgggag ccaatttatt gtgaccgtgg ttttcccggc
      901 gttctccgtg aacttggctg tggaaagcat ttccgcgggc cagttgggca tcatcgggtg
      961 ccgggccatg ttgccgtcct tgtctgaaaa tgagttgacc cagacgatcc gttccggcgc
     1021 gacgatttcg cgataaacaa atttgcccca catttcttta ccgtctggag agcgcaggca
     1081 gtagtgaaaa tttccacccg ggcgcaaatc caatctggcg ctggtcatgg cgaaaccttt
     1141 cggcccaaac cattgcatca ggcgttcgcg ttccgtccac gccttgaaca caagctcgcg
     1201 cggcgcgtcg aaggtgcgtg agatgataaa gggttccatg ccggtattga ctgtggattc
     1261 agcgggattg ttttttcttg cggtcatttt gagttttgtg ttgttttgtt tttgcttgaa
     1321 gttcctgtag ataatcgtcc agacggtcta agctttcttc ccagaaccgt cggtaatgtt
     1381 ccagccaatc caccgcgtcc ttgagcggtt tggccttgag acggcaggga cgccattggg
     1441 ccgcgcggct gcgcacgatc aatccgctgc gttcgagcac gcgcaaatgt ttggaaacgg
     1501 cgggcaagct catgtcgaag ggccgcgcga gttccttcac cgaaacttca cccgaggtga
     1561 gccgcgaaag gatggcccgc cgggtgggat cggccaatgc cgcgaaagtg acgctgagat
     1621 ggtcggttgt catttgtaat aaaccgtatg gttaattaac ttattagtta aatacaacaa
     1681 gaaataaaca ctgtcaactt tttctttgaa ttttttgcat tcgaggtcct gtgcgcaata
     1741 tcggttcctt tgcagaatta actcgttcgc acggctttcc cggacctctt atctgtcatg
     1801 tcgggaattg gccagtccaa actgattgta tttctcccgc atatccacat actttgcgaa
     1861 gcttggaaaa gagcttgtag gtttttgaaa tatgaaaatg gcatggtcgt tccgcccgcg
     1921 attggcggac acattgaaaa actattcgcg cgagcaattc atgtccgatg cgatcgccgg
     1981 attcacggtg ggcatcgtgg cgttgccgct ggcgatggcg ttcggcatcg cgtcgggcgt
     2041 cacgccgcag gcgggcattt tcacggcggt ggtcgccggt ttcatcatct cggcgttggg
     2101 cggttcgcgc gtgcaaatcg gcgggccgac gggcgcgttt gtgagcatcg tgctcgccac
     2161 gattgtgact tacggactgg acaatcttta catctgcacg gtgttggcgg ggttgatgct
     2221 gctggtgatg ggtttttgca agatgggcaa tctcatcaag ttcattccat ttcccgtcac
     2281 gatgggtttt acctgcggaa tcgcggtgct catcttcagc acgcaaatca aagacttttt
     2341 gggactgcaa gtcgagcatg tgccgggcga tttcgtcggc aagctgcaaa tgctctatga
     2401 aaatatcggc gcgattgact ggcccacggt ggtgctggcg gttgcttcga taattgtcat
     2461 caagacttgg ccggcaaaac tggcaaagcg agtgccgggc tcgattgtgg ctttgttgct
     2521 aggaacagta gtggtgacgg tctgccattt gaaagtagca accattggct cggtcttcgg
     2581 cgggattccg acaggattgc cgaagattca tttgccgccg ttgtcatggg agaaaattca
     2641 acatctgatt gagcccgcat ttacgattgc attgctggcg gcgattgaat cgctgttgtc
     2701 cgccgttgtc gcggacggga tgatcgagga caggcatgat tccaaccagg aattgatcgc
     2761 gcagggtttg gcgaacattg ccagcccgtt tttcggcgga atccccgcga ccggcgccat
     2821 cgcgcgcact gcgaccaacg tgcgttgcgg tgggcgaagt ccgattgcag gcatcacgca
     2881 cgcggtcacg cttttgataa ttattctcgt ggctgcgccg ttggcgaagt acattccgct
     2941 cgcggtgttg agcgcggtgc tggtggtggt ggcttacaac atgggcgaat ggcatcaatt
     3001 ccgccggctg cgaaaatggc ccaggagcga ttcggcggta tttctcgttg tgtttgcgct
     3061 caccgttttc accgacctgc cgaccgctgt gaaaatcggg atggtgcttg cggcggcgct
     3121 gttcgtgaac cgcatttcgc agacgatgca aatcaccgcc gtggaccaaa gcaccagcgc
     3181 cgaaagcgcg cacaattccg tcatcggcaa ggaagtgccg aagggtgtga tggtctatcg
     3241 cgtgttcggc gcgtttttat tcggcgcggc ggacaagctg gagaccgcgc tcaagcgcgc
     3301 ccaccaggag ccggagattt tgattttgaa aatgcagagc gtgctcgcga tggatgccac
     3361 cggtttgaat gcgctcgaag acatttacga aaaacttcat cgcaagggca ggcatctcat
     3421 cctcagcggg ccgcacacac agccgttggt gacgatgcaa cgggacgggt tcattgacca
     3481 attgggcgcg gaaaatgttt gtccgcatct cgacgcggca ttggaacgcg cgcgggaaat
     3541 ccttgatgcg aaggccgcaa aaaacgtgaa cgtcatcccg aagcccgcca ctgtctaggt
     3601 agcgcagact tgcagtctgc tgtatcgcgg attttcaatc cgcaaggtgc gcaatgttca
     3661 gccctgcgag ataaacgcct tgccgactgc aactggcgaa acagcagatt aaaaatctgc
     3721 gctgcctcca agggggttca cttgccagaa ccattcgatt catgcgcgcg ataattgcca
     3781 tccagttcgg ccgcaaggta agtgaacacc gcaaacgcgg cggtgctttg gttgaggcgt
     3841 tcgggcttta tcttgtccac ggtgtcgtcg gctgtgtgat gcaaatcgaa ataatcggtc
     3901 gcgtccattt ccaaggtgac cgccgggaca tgcgcctcgg ccagcggttc gacatccgaa
     3961 ctgccgcggg attggttgcc gcccgggaga atgcccaacg gtgccaaagc cgcgcgaatg
     4021 gtcttgagag tcggttcgtc tggatttgcg acgccagtct caaatctgta aatcggccct
     4081 tgtcccaaat ccggttccgc aacgataatg tatttcgaca attcagcgtg atggctcgcc
     4141 gcgtaagctc gtccgccgag aagtcccatt tcttcggagc cgaacaacac cacgcgaatc
     4201 gtgcgcctcg gccgttgtgg gagatcgtgg atcaattttg tcgccgccat cacgatggcg
     4261 acaccggctc cgtcgtcaat cgcgccagtg cccaaatccc acgagtccag gtgcgcgcca
     4321 agcaacacga tttcgtccgg tttttcgcgg cctttaagtt cggcgatcac attttggctc
     4381 gcgaccgtgc cgaggtcacg cggcgtgagg acgagtttga ccttcaccgg ctcgccaagc
     4441 gcaaccagac ggtccaactg ttcggcgtcc ggaacggaaa gcgccgcggc cggaatccgt
     4501 gggccgtcga tcggaaaatg cgtgttgccc gtgtgaggca atcggcggct atcggtcgcc
     4561 agcgaacgga gcaaatatcc gacagcgccg cgtttcgccg cttccgcagg accgaggctg
     4621 cgaatcttgt tggcttcaga ataaccggag gcgtcctgcg tgcggaccat cggttgcgtg
     4681 accaccgcga tttttccctt gagcgaaccg gcaggcgcgg caagcaactc atcgtaagtc
     4741 ttgaacaaaa caatttccgc cgtgagaccc tccggcggcg tcgcggggct gccgcccaac
     4801 gcggtgacag caagtcgttg t
//
LOCUS       DASZMM010000602         5947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_3571303, whole genome shotgun sequence.
ACCESSION   DASZMM010000602 DASZMM010000000
VERSION     DASZMM010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3571303"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /gene="ychF"
                     /locus_tag="VG754_14545"
     CDS             <1..1014
                     /gene="ychF"
                     /locus_tag="VG754_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015220828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWF20487.1"
                     /translation="NYPFCTIDPNVGIVTVPDTRLEELRKIAKTSVVIPAAIEFVDIA
                     GLVKGAAQGEGLGNKFLTHIREVDAIVQVVRCFEDPDIHHVAGAIDPIRDIETIITEL
                     VLADLETATKRQEKGAKDAKRGDKTAVAEAEVFAKLVPHLNAGKPAITLELSPEEKAI
                     ARGFFLLTDKPTIFAANVKESDLATADSNPYVKKVRDYARTHIACETVVVSAQIESDL
                     VDLSEDEAKEFLKELGVQESGMGALIRSTYHLLGLRTYFTAGEKEVRAWTIHAGDTAP
                     KAAGVIHSDFERGFIKAETVAYDDLVKCGSVAAAREKGLYRMEGKEYVVKDGDVMLFK
                     FNV"
     gene            1193..1885
                     /locus_tag="VG754_14550"
     CDS             1193..1885
                     /locus_tag="VG754_14550"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HWF20488.1"
                     /translation="MAPFVVFVLLTALQGHWGEASRYWLYLIKTAVGIGLIWLMRPVV
                     TEMRWAFSWEAVVVGVAVFAIWVGLDGHYPSFDQIIQNYLCPALKSAGLEKWCPQPAA
                     NKLPWNPNAQFGPGTPLAMLFIITRILGSSLVVPPLEEVFYRSFLYRYIVKPDFLAVS
                     FGTFHAFAFVVTAAIFGFSHYEWLAGILCGFAYQGLVLWKKRLGDAMTAHAITNFLLG
                     VWIVWRGAWHFW"
     gene            1858..3498
                     /locus_tag="VG754_14555"
     CDS             1858..3498
                     /locus_tag="VG754_14555"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWF20489.1"
                     /translation="MAWRLAFLVKALILFEDDHLLVVNKPAGMNTHAPSPFAGEGFHE
                     WLRHREPRWSRLAIIHRLDKETSGVMVFGKTALANRSLTEQFTQRAVHKKYRFLTDRP
                     IDFQKRTMISSLVRVGEKYVSRPLHAGAERAETRFHVLETKSGRTLLEAEPITGRTHQ
                     IRVHAAAESFPILGDTLYGGTAAKRVCLHAVELTLKHPLTGGEMTFSTPEDFDSDARL
                     ELRELIMSARAENSRFQLQDTNAYRLIHGAADSWAGWYVERLGDYLLSQAEKSLNAAQ
                     EKELRQMMKIYNARGAYHKILTRHVRRMNVTQASPQLVLGEAAPGAFTILENGMQFEL
                     SFDEGYSVGLFLDQRDNRRRLLTNHVAADFPLFPEHVAGTEVLNSFAYTCGFSVCAAK
                     AGARVTSLDLSKKYLEWGKRNFVLNKLDPTAHDFIYGDAFDWLRRLEKKGRAFDAVLL
                     DPPTFSQSKSAGVFQAEKNFDRLVKAALPVLKPGGVLFASTNATRLEPEQFLETVNAS
                     ISSAGRKILQQHYAPQPPDFPVHRDEPAYLKTVWMRVG"
     gene            complement(3512..5017)
                     /locus_tag="VG754_14560"
     CDS             complement(3512..5017)
                     /locus_tag="VG754_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF20490.1"
                     /translation="MRLKSNQQPDRSGIREPGIAHRHSAIRILFSALLLLIIGPLAPQ
                     LFAQPKTSVPPPVSNRFLFIVDTSSAMKRHQQDALKIVYDILSNAATGQMHSGDSLGL
                     WTFNQDVYSDLPAQTWRAENHDQIIQRTLTFLQQQRYGKTSRFDKALGDMQDVIRASD
                     VITIFLISTGHNKISGTPFDSEINALYKQTVADMKREPKPVVTVLQAKKGKLIHFTVS
                     APPWPVVIPEVPIPIRVAQSAPQNPPQATAPKPTAPRAPPSATNALIIRPVVHQSRPV
                     PPSPSPNQVAPAAPPAIQNPVPPPVPQVSVPPPLPVNPQSTPAITTAPPVAPTSAPAA
                     SIPTAPPPISQATPVPPNVLPPNLAIQHAPPNFIPPPPAVDNPVRPVIPAAQAPLLSQ
                     SVPARSNTVAVVPVKSAPLPSVAPATNTVAPAESTSSTPIVTAAPGTNSVSAKALAFV
                     TPETTTSPKTFLIAGLALLLVGIVLIIVMVRRSRAASGPSLISQTMGNGKK"
     gene            complement(5313..5783)
                     /locus_tag="VG754_14565"
     CDS             complement(5313..5783)
                     /locus_tag="VG754_14565"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWF20491.1"
                     /translation="MALVRILVDGYSLLHGWPELAPGKPRHSAAARDELIHRLTLYRD
                     AIGTPITIVFDGANNDIRLSTVESTPEMEILYSRAGQTADDIIERVAHRLSAYGEVLA
                     VTDDFAERDTVIAMGGMASSCLNFIQTVEAALAELQDDIKTYNRDQRARFRRPK"
     gene            5866..>5947
                     /locus_tag="VG754_14570"
     CDS             5866..>5947
                     /locus_tag="VG754_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWF20492.1"
                     /translation="MKTKEPIPVTVLTGYLGAGKTTLLNHL"
BASE COUNT         1356 a         1457 c         1780 g         1354 t
ORIGIN      
        1 aactatcctt tttgcacgat tgatcccaac gtgggcatcg tcactgtgcc ggacacgcga
       61 ttggaggaat tgcgaaagat cgcgaagacc agcgtggtca ttcccgccgc gattgaattt
      121 gtggacatcg ccgggctggt caagggcgca gcgcagggtg aaggtctggg caacaaattt
      181 ctcacgcata tccgcgaggt cgatgccatc gtccaggtcg tgcggtgctt cgaggacccg
      241 gacatccatc atgtcgccgg cgcgattgat cccatccgcg acatcgaaac catcatcacc
      301 gagttggtgc tcgccgattt ggaaactgcc accaaacgcc aggaaaaagg cgccaaagac
      361 gccaagcgcg gcgacaagac cgccgttgcc gaagctgaag tttttgcgaa actcgtgccg
      421 catctcaacg cgggcaagcc ggccatcact cttgaattgt cccctgagga aaaagccatc
      481 gcccgcggct tctttttgct caccgacaag ccgaccattt tcgccgcaaa tgtgaaggag
      541 tccgacctcg cgaccgctga cagcaatcct tacgtcaaaa aggttcgcga ttatgccagg
      601 acgcatatcg cctgcgagac ggtggttgtc agcgcgcaga tcgagagcga tttggtggat
      661 ttgtcagagg acgaagcgaa agaatttttg aaggaactgg gagttcagga atcgggcatg
      721 ggcgcgctga tccgcagcac gtatcatttg cttggattgc ggacgtattt caccgcgggt
      781 gaaaaggaag tccgcgcgtg gaccatccat gccggcgata ccgcgcccaa ggccgcgggc
      841 gtgattcata gcgatttcga acgcggcttc atcaaggctg aaactgttgc ttacgatgat
      901 ttggtcaagt gcgggtctgt cgccgccgcg cgcgagaaag gactttaccg catggaaggc
      961 aaggaatacg tggtgaagga cggcgacgtg atgctcttca agttcaacgt gtaagaaagg
     1021 agaacgctga acatggccgg caaatcttca aaaaaggaac cgcaattgaa gccgcggatg
     1081 cgccggacga tccgcacgaa gttcgtaaat aaggtgtggc gcggcgatgt ttagctttcg
     1141 ccgcgtgaat ccagtctcaa aaaaaataca gggatcgccc gctctggcgc gagtggcgcc
     1201 gttcgttgtg tttgtcttgc tcaccgcgtt gcaaggccat tggggcgaag cttcgcgtta
     1261 ctggctctac ctgatcaaaa ccgctgtcgg catcggatta atctggctga tgcggcccgt
     1321 cgtcacagaa atgcgctggg cattcagttg ggaagccgtc gttgtaggcg ttgcagtgtt
     1381 tgcgatatgg gtcggacttg atggccatta tccgagcttt gatcaaatca tccaaaatta
     1441 tttgtgtccg gcattgaaat cagccggctt ggaaaaatgg tgcccgcaac cggcggcgaa
     1501 caaactgcca tggaacccga acgcgcaatt cggaccagga acaccgctgg caatgttgtt
     1561 tatcatcacg cgcattcttg gttcgtcgct tgtcgtccca ccgctcgagg aagtttttta
     1621 ccgttcgttt ttataccggt atattgtgaa gccggatttt ttggcggtgt cgtttgggac
     1681 ttttcatgcg ttcgcgttcg tggtgactgc ggcgattttc ggtttcagcc attatgaatg
     1741 gttggcggga attttgtgcg ggttcgctta tcaagggctg gtgttatgga aaaaacggct
     1801 tggtgacgcc atgacagccc atgcgatcac gaatttcctg ctcggtgttt ggatcgtgtg
     1861 gcgtggcgcc tggcattttt ggtgaaggca ttgatcctgt ttgaagatga ccatttgctg
     1921 gtggtgaaca aaccggcggg gatgaacacg cacgcgccca gcccgtttgc cggcgaaggt
     1981 ttccacgaat ggctgcggca tcgcgagccg cgttggtcaa ggctcgccat catccatcgc
     2041 ctcgacaagg aaacttccgg cgtgatggtt tttggcaaaa cggctttggc caatcgctcg
     2101 cttaccgaac agttcacgca acgagccgtc cataaaaaat accgatttct cacggaccgt
     2161 ccgattgatt ttcaaaagcg cacgatgatt tcgtccttgg ttcgcgtggg agaaaaatat
     2221 gtgagccgtc cattgcacgc gggcgccgaa cgcgcagaga cgcgttttca tgtgttggaa
     2281 accaaaagcg ggcggacact tttggaagcc gagccaatca caggacggac gcatcaaatc
     2341 cgagttcatg ctgctgccga aagttttcca atcttgggag acacactgta cggcggcacg
     2401 gccgctaaac gggtctgttt gcacgcagtg gaattgacgc tgaaacatcc gttgactggc
     2461 ggagagatga cgttcagcac gccggaagat ttcgactcgg atgcgaggct tgagttgcgc
     2521 gaattgatca tgagcgcgag ggctgaaaac tcacgttttc agctacagga tacaaacgca
     2581 taccggttga ttcatggggc ggcggacagt tgggcgggat ggtatgtgga gcgattgggt
     2641 gattatcttt tgtcgcaggc ggagaagtcg ttgaacgcgg cgcaagagaa ggaactgcgg
     2701 caaatgatga aaatttacaa cgcgcgtgga gcatatcaca aaatcctcac gcggcatgtg
     2761 cgccggatga acgtgacgca ggcttcgccg caattggttt tgggcgaggc tgcgcccggc
     2821 gcgttcacaa tcctggaaaa cggcatgcag ttcgagttga gctttgacga aggttattcg
     2881 gttggattgt ttttggatca acgggacaat cggcggcgat tgctgacgaa tcatgtcgcc
     2941 gcggattttc cactctttcc tgaacatgtg gcaggcacgg aagtgttgaa ctcgtttgcg
     3001 tacacatgcg gtttttcggt ttgcgcggcc aaagcgggcg ctcgcgtcac cagcctcgat
     3061 ctttcaaaaa aatatctcga atggggcaaa cggaatttcg tccttaacaa gctcgacccc
     3121 accgcccacg atttcattta cggcgacgct ttcgactggc tgcggcggct cgaaaagaag
     3181 gggcgcgctt tcgatgccgt gctgctcgac ccgcccacat tctcgcaatc caaatcggcg
     3241 ggtgtgtttc aggcggaaaa aaatttcgac cggctggtga aggccgcgct gccggtattg
     3301 aaaccgggtg gtgtattgtt cgcttcgacc aatgccacgc gcctggagcc tgaacaattt
     3361 ttggaaaccg tcaacgcatc catcagctcc gctggacgaa aaatcttgca acaacattac
     3421 gctccgcaac cgcccgattt tccagtgcac cgcgacgaac ctgcttattt gaaaacggtc
     3481 tggatgaggg ttggttaaga ttgtccaaca ttcatttctt gccgtttccc atcgtctgag
     3541 aaatcaggct cgggccggaa gccgcccgtg aacggcgcac cataacaatg atgagcacga
     3601 ttccaaccag cagcagagcc agccctgcga tcaaaaaagt tttcggactg gtcgttgttt
     3661 caggggtgac gaaggccagc gcttttgcag aaaccgagtt tgttccgggt gcggcagtga
     3721 caatcggagt ggaagacgtt gattcggcag gtgcgacagt attcgttgca ggagcaaccg
     3781 atggcagtgg tgcagattta acaggaacga cagccacagt atttgatcga gccggaacag
     3841 actgcgataa aaggggcgct tgtgccgcgg gaatcacggg acgaacggga ttatcaaccg
     3901 cgggcggcgg gggtatgaaa ttcggcggag cgtgttgaat ggcaagattg ggagggagca
     3961 cgttcggagg aaccggcgtc gcctgcgaaa tgggcggtgg agcggttgga attgaagcag
     4021 ccggtgccga tgttggcgca actggaggag cggtggtaat ggcgggcgtg ctttgcgggt
     4081 tgactggcaa cggaggcgga acggacactt gaggaacagg tggtggaacc gggttctgaa
     4141 ttgcgggagg tgccgcgggt gcaacttgat ttggggacgg cgatggcgga acgggccggg
     4201 attggtgaac cacaggacga attatcaagg cgttcgtcgc agacggaggc gctctcggcg
     4261 cagtcggttt cggggcggtt gcctggggtg ggttttgcgg cgcagattgt gcaacccgga
     4321 tgggtattgg cacttccgga atcacgaccg gccaaggtgg ggcgctgacc gtgaaatgaa
     4381 tgagcttgcc ttttttggcc tgcaaaactg tgaccaccgg ctttggctcc cgtttcatat
     4441 cggcaacagt ctgtttataa agcgcattaa tttcgctgtc gaacggtgtt ccagaaattt
     4501 tgttgtggcc agtcgagata aggaaaatgg tgatgacgtc cgaagcgcga atgacatctt
     4561 gcatgtcgcc aagggcctta tcgaaccggc tcgttttgcc gtaacgctgt tgctgcagga
     4621 aagtcagcgt gcgctgaatg atttggtcat gattttcagc tctccaagtc tgcgccggaa
     4681 ggtctgaata gacgtcctgg ttgaaagtcc acagaccgag cgaatcaccg gaatgcatct
     4741 ggccggtggc ggcgtttgaa agaatgtcat acacaatctt aagcgcgtct tgttggtgac
     4801 gcttcatcgc cgacgaagta tcaacgatga acaggaagcg attggaaact ggcggaggga
     4861 cggaagtttt gggttgggca aaaagttgcg gcgccagcgg tccgataatc aacaatagca
     4921 atgcagaaaa taaaatgcgg attgcggaat ggcgatgggc aatcccgggc tcccggatgc
     4981 cactgcggtc aggctgctgg ttcgatttca ggcgcattga taattttttt ggcaacagga
     5041 tgacagcgcg attcattgac agcctggtct ccgcttttgg ggagcaataa aagaatacga
     5101 gatggcgggt tgaaccgcaa ttgcggattt ggaatgggtc cttcccaacc acttccccaa
     5161 ttggcccgcc aaaataattt tttattttat cgcggtttat ccccatcaag cccggttgag
     5221 gtgtcctatg tcgcgctcct tgtcgcaagc ggtttgaatt atttcacaga acaacagttt
     5281 atcaatcttt ttggcaccac tctacaagcg aattattttg gccggcgaaa acgggcgcgc
     5341 tgatctcgat tgtaggtctt gatgtcatcc tgaagttcgg cgagcgcggc ttcgacggtc
     5401 tggatgaaat tcaggcagct tgatgccatg ccgcccattg cgatgaccgt gtcgcgctcc
     5461 gcaaaatcat ccgtcaccgc cagcacttcg ccataagcgc tgagacgatg cgcgacacgt
     5521 tcgatgatgt catcagcggt ctgcccggcg cgggaataaa gtatttccat ttcgggtgtg
     5581 gattcgaccg tggagaggcg aatatcatta ttcgcgccat cgaagacaat ggtgatgggc
     5641 gtaccgatgg cgtcgcgata caaggtgaga cggtggatca attcatcgcg tgcggcggcg
     5701 gaatgtcgcg gcttgcccgg ggcaagttcc ggccagccgt gcagcaggct gtagccatcg
     5761 acaaggatgc gcaccaaagc catccctcaa ccttaattga aaacgcaaaa tgtaaaatca
     5821 aaaaattatt cggttaattc ggtccattct gtcaaaatat cttcgatgaa aacgaaagaa
     5881 ccgattcccg tgacggtgtt gactggttat ctgggcgcgg gcaaaaccac attgctcaat
     5941 catttgc
//
LOCUS       DASZMM010000603         5474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U5891
            k141_2198045, whole genome shotgun sequence.
ACCESSION   DASZMM010000603 DASZMM010000000
VERSION     DASZMM010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956711
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:35:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,918
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198045"
                     /isolate="SMAG_U5891"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VG754_14575"
     CDS             complement(<1..607)
                     /locus_tag="VG754_14575"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF20493.1"
                     /translation="MSDPSALAQEKKLMAADLIIINATVHTMDPAQPTAEAVALLGNR
                     IAAVGSTDEIRSLAGPKTRVIDAHQRLVLPGFNDSHVHFLSGGFQLFNVDLRDAKSPR
                     EFAERIHRFAERIPKGQWILGGEWDHERWLGAPLPTKELVDAATTNNPVFVSRMDGHM
                     ALANGLALKMAGVTRDTQEVDAGKIVRDPKTGEPTGLLKDAA"
     gene            complement(860..1537)
                     /locus_tag="VG754_14580"
     CDS             complement(860..1537)
                     /locus_tag="VG754_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF20494.1"
                     /translation="MQKITVLLADDHTVVRQGLRALLVVEEDIEIVGEAETGRQAVVM
                     AKKLMPDVVVMDIAMPLLNGLEATRQITKQLPDTKVLVLSSYSDDEYVQQLTEAGAAG
                     YLVKHTAANDLIKAIREARRGNAFFSPSIAKRLRDQCREAFVSGQPVRKRTDYLTSRE
                     SEVLQLIAEGQANKQIAAELCISIKTVEKHRQQVMNKLNIHDVAGLTRHAISKGIIES
                     NATAKPI"
     gene            complement(1634..2788)
                     /locus_tag="VG754_14585"
     CDS             complement(1634..2788)
                     /locus_tag="VG754_14585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWF20495.1"
                     /translation="MTNSGGQLARQYMAALRKFLANKNERALHQAYELGRKTIAGGLG
                     VIDMAKIHEDVLQAILPKTTSAKESLRLARNAGTFLAESLSPFEMTHRGFRNANETLR
                     ELNEALEEKNHQLASANHKLKREISVRKQVEKELRESEEHYRELLVQARMMQENLRYL
                     SCQILNVQEEERKRISRELHDEVGQALTAVNVNLAVLKKAASGSASHLLQKINDAQGL
                     LERTMKTVHDFSRELRPALLDDLGLIPALRSFVKDFSERTSVRVNFSSSSEVEKMETD
                     QKTVFYRVAQESLTNISKHAQATESRITLRRDKKGIRMEITDNGKSFRVEQHLSPEVK
                     GKKRLGLLGIQERVRLANGKCEVESTPGKGTRVRIWIPFRIGGFSGGQLE"
     gene            3141..>5474
                     /locus_tag="VG754_14590"
     CDS             3141..>5474
                     /locus_tag="VG754_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HWF20496.1"
                     /translation="MFVQAAETAPAPDGASLALPKAGEMELRCITPSLLEVTLVTTQP
                     KSTGPQWNFVSDDGRLTLPGTNEFVVTVSGSNCPVVAIGFKRRVLYAPLKQHDIRVGN
                     HLYLKLSHAIADGQTVQVKNPDAKLWPASQRLVVTCDPLRWSPAIHVNEVGYLPELPK
                     KAMVGFYLGSLGEMDFQSSNTFQLVDAANGRRVFEGKLAPRPDHGFPFANYQAVLEAD
                     FSAVKTRGEYRVMVPGLGASFPFFISDEIAGAFARTYALGLYHQRCGTSNSLPFTRFT
                     HDACHIAPAQIPVPQSKFQFTWETIASTDADFASEPRHTAPQLKDEASQLYPFVKRGK
                     INVSGGHHDAGDYSKYTINSAGLIHFLVFAADNFPGAGALDNLGLPESGDGRSDLLQE
                     AKWEADFLARMQDDDGGFFFLVYPREREYEIDVLPDHGDPQVVWPKNTAVTAAATAAL
                     AQCASSPLFKKQFPEAAALYLQKARAGWAFLDRAIAAHGRDGAYQKLTHYGNDFMHDD
                     ELAWAACEMFAATGDDSYHQKVLDWLKPSGDTRLWGWWRMYGSWGCAIRDYAFAASSG
                     RIKREKLDPQLLSACEDEIIANAQDQLHRSQDCAYGTSFPEETKRIRSGGWYFSGDAA
                     FDLAVACQLDYPIFNDPRPKYLDAIIANLNFEAGCNPVNICYLTGLGWKRPQEIVHQY
                     SQNQRRALPPDGIPIGNLQAGYSWISQYQNELTALSFPSDGDETAPYAFYDRWADTFN
                     LTTEFVILNQGRSLAYLAWLMARTPLKNQPWRSADARI"
BASE COUNT         1137 a         1538 c         1493 g         1306 t
ORIGIN      
        1 tcgcggcgtc cttcaaaaga ccggttggtt cgccggtctt gggatcgcgg acaatttttc
       61 cggcgtcaac ttcctgggta tcgcgggtga cacccgccat cttcaaggcg aggccgttcg
      121 cgagcgccat gtggccatcc atgcggctga caaaaaccgg attgttcgtc gtcgcggcat
      181 ccaccaattc cttggttggc aacggcgcgc ccagccagcg ttcatggtcc cattcgccgc
      241 caaggatcca ctggcctttg ggaattcttt cggcaaaacg atggatgcgc tcggcaaatt
      301 ctcgcggaga ttttgcatcg cgcaaatcca cattgaaaag ttgaaaaccg ccgctgagga
      361 aatgaacatg ggaatcgttg aatcccggca gcacaaggcg ttggtgggcg tcaatgacgc
      421 gtgttttggg gccggcgagg gaacggattt catcagtgga accgacagcg gcaatgcgat
      481 ttccgaggag ggcgaccgct tcggcagtcg gttgcgccgg gtccatggta tggacggtgg
      541 cgttgatgat gatgagatcg gccgccatca actttttttc ctgcgcgagc gcggatgggt
      601 cagacatggc aaaggacgct atgccgagag cgaaaaggat ggaagtaaaa aaggagtcag
      661 acaacagctt gcaaacagac gccatgctca tgacattccg cccagacatg cgtttcgtta
      721 taacgcaacg gcgcagaggc gcaaagacgc aacggtgata accaataaat ccaggcggtc
      781 gtttgcgctt ggccaaatgt gctttgcttc cctgcgtctt cgcgccttcg cgtctccgcg
      841 ttttgccggg tttaaaatat cagatcggct tggcggtggc gttgctctcg atgattcctt
      901 tggagatggc gtggcgcgtg agaccggcga catcatggat gttcagcttg ttcatcacct
      961 gctggcgatg tttctcaacc gtcttgatgc tgatgcacaa ttctgcggcg atctgcttgt
     1021 tggcctggcc ttcggcgatg agctgcaaca cctcggactc gcgggaagtg agatagtcgg
     1081 tgcgtttccg gacgggttgg ccgctgacga aggcttcgcg gcattggtcg cgcaaacgtt
     1141 tggcgatgga cgggctgaaa aacgcattgc ctctcctggc ttcgcggatg gccttgatca
     1201 aatcgttcgc ggcagtgtgc ttgacgagat aaccggcggc gccggcttcg gtcagttgct
     1261 gcacatattc gtcatcactg taggaagaaa gcacgagcac cttggtatcg ggcagttgct
     1321 tcgtgatctg gcgcgtggct tcgaggccgt tcaagagcgg catcgcgatg tccatcacca
     1381 caacatcggg catcaatttc tttgccatca cgaccgcctg acggccggtc tctgcttctc
     1441 caacgatttc aatgtcctcc tccaccacca gcaacgcccg cagcccctgc cgcacgacgg
     1501 tatggtcgtc cgccaacaaa acagtaattt tttgcatagg ttgataggtt gtttgattgt
     1561 ctgccgttat ctgatttatt tgcaaatacg aacggaacaa atctgtcaaa cgccgctgcg
     1621 gcaccgcttg agtttattcc aattggccgc cggaaaatcc gccgatccgg aagggaatcc
     1681 agatccgcac cctcgtgcct ttgccgggag tggattccac ttcgcattta ccattggcga
     1741 ggcggacccg ctcctgaatc ccaagaagcc cgagccgttt tttgcctttg acctcgggcg
     1801 aaagatgctg ctcgacccgg aacgacttgc cattgtcggt gatctccatg cggatgcctt
     1861 ttttatcacg gcgcagtgtg atgcgcgact ctgtggcttg cgcgtgcttg gaaatattcg
     1921 tcaggctttc ctgcgcgacc cggtaaaaga ccgtcttctg gtcagtctcc atcttttcga
     1981 cttccgaact ggagctgaaa ttcacccgca cactggtgcg ctccgaaaaa tctttgacga
     2041 aggagcgcag tgcgggaatc aatcccaggt cgtccagcaa ggcggggcgc aattcgcgcg
     2101 aaaaatcgtg gacggttttc atcgtccgct ccagcaggcc ctgggcgtcg ttgatttttt
     2161 gaagcaaatg gctggcgctg ccgctggcgg ccttcttgag cacggcaagg ttcacgttca
     2221 ctgcggtcaa tgcctggccg acttcgtcgt ggagttcgcg gctgatgcgt ttgcgttcct
     2281 cttcctgcac gttcaggatt tggcaggaga gatagcgaag gttttcctgc atcatgcgcg
     2341 cctgcaccag caattcgcgg taatgctcct cgctttcccg caattctttc tcgacctgtt
     2401 ttcgcacgga gatttcgcgt ttcaatttat ggtttgcgga ggccaattga tgattttttt
     2461 cttccaaagc ttcgttcaat tcacggaggg tttcgttcgc gttgcggaaa ccgcgatggg
     2521 tcatttcaaa tggcgaaagg ctctcggcca aaaacgtccc ggcattgcgg gccaggcgca
     2581 ggctttcctt tgcgctggtg gttttgggaa gaatggcctg cagcacatct tcgtggatct
     2641 tcgccatgtc gatgactccc aaaccgccgg caatggtctt gcgccccaat tcataggctt
     2701 ggtgcagggc gcgctcgttt ttgttcgcga ggaacttccg caaagccgcc atgtactgcc
     2761 gtgcaagttg gccgccggag ttagtcatta ttgccagtca aagataatgc attgccgtga
     2821 actgtcccgt gtccacgatt tgatcccgaa cgaaattacc gccacgcttc aacccacgct
     2881 cagtttaccg tggttggttg cgtcaagtct caaagtcatt agcatgaatt gtgctcaccc
     2941 gcaaaacccg cgggaaatct cccggatggt cttacgtttg acgatattta aacatggggt
     3001 gaaaccctaa ttatactttt gtggccaacg acttgtgcaa gttcgaaggt tgcgcgaaat
     3061 tagccgccat catgtatcat tcatcccttt ttcgcaaatt gcattcggca ttttgggccg
     3121 ctgttttatt gcaaattgcc atgttcgttc aagccgccga aacagcaccc gcgcctgatg
     3181 gcgcgtcgct ggccctgccc aaagccggtg aaatggaatt acgctgcatc actccatcct
     3241 tgctcgaggt cacgctggtc acgacgcaac cgaaatcgac cgggccgcaa tggaattttg
     3301 tgagcgacga tggccggctg actttgccgg gcacgaatga atttgtggta acggtttccg
     3361 gaagcaattg tccggtcgtc gcaatcggtt tcaagcgacg cgtcctctat gcgccactca
     3421 aacagcacga catccgcgtc ggcaatcatc tttatctcaa gctgtcgcat gcgattgccg
     3481 atggtcaaac ggtgcaggtg aaaaatcctg acgcgaaact ttggcctgcc agccagcggc
     3541 tcgtcgtcac gtgcgatcca ttgcgctgga gtccggcaat ccatgtgaac gaagtcggat
     3601 atctgcccga acttccaaag aaggcgatgg ttggatttta cttgggcagc cttggcgaaa
     3661 tggattttca atcgtcaaac acttttcaac ttgttgacgc cgcgaatggc cggcgggttt
     3721 ttgaaggcaa gcttgctcca agacccgacc acggttttcc attcgccaat tatcaagcag
     3781 tgcttgaagc agatttttcc gcagtcaaga cacgcggcga gtatcgcgtg atggtccccg
     3841 gacttggcgc gtcatttccg tttttcatca gtgacgaaat cgccggtgcg ttcgcgcgga
     3901 cttatgcgtt gggactttat catcagcgtt gcggcacgag caattccctg cccttcaccc
     3961 gtttcactca cgacgcgtgc cacatcgcgc cggcgcaaat tcctgttccg caatccaagt
     4021 ttcagttcac ctgggaaacc attgccagca ccgacgccga ctttgcgtca gaaccacgtc
     4081 acaccgcccc gcaacttaag gatgaagcct cgcaacttta tcctttcgta aagcgaggca
     4141 aaatcaacgt gagcggcggg catcatgatg cgggcgatta cagcaaatac accatcaaca
     4201 gcgccgggct gatccatttt ttggtgttcg ccgccgataa ttttcccggc gcgggcgcgc
     4261 ttgacaatct cggcctgccc gaaagcggcg atggccgtag cgatcttttg caggaagcga
     4321 aatgggaagc cgattttctc gcccggatgc aggatgacga cggcggattt ttctttctcg
     4381 tttatccgcg cgaacgcgaa tacgaaatcg atgttctgcc cgaccacggc gatccccagg
     4441 ttgtctggcc caagaacacc gctgtcaccg ccgccgcaac cgccgctctc gcccaatgcg
     4501 cttcgtcgcc tcttttcaaa aaacaatttc cggaagccgc ggcgctctat ctgcaaaagg
     4561 cccgcgctgg ctgggctttt cttgaccggg caatcgcggc gcacgggcgc gacggcgcgt
     4621 atcaaaaatt gacgcattac gggaacgatt tcatgcatga cgacgaactg gcttgggcag
     4681 cttgcgaaat gttcgccgcg accggcgatg actcgtatca tcaaaaagtg ctcgactggc
     4741 tcaaaccctc cggcgacacg cggctttggg ggtggtggcg catgtatggt tcgtggggtt
     4801 gcgccatccg cgattacgct ttcgctgcga gttcaggtcg aatcaaacgc gaaaagctcg
     4861 acccgcaatt gctctcagct tgcgaagacg aaatcattgc gaatgcgcag gaccagttgc
     4921 atcgctccca ggattgcgct tacggcacga gttttcccga agaaacaaag cgcattcgca
     4981 gtggcggatg gtatttttcc ggagacgccg cttttgattt ggctgtcgcg tgccagctcg
     5041 attatccaat cttcaacgac ccacgaccaa agtatctgga tgccatcata gccaatctaa
     5101 acttcgaggc gggctgcaat ccggtgaata tctgctacct gaccggcctc ggctggaaac
     5161 ggcctcaaga aatcgttcat caatactcgc aaaaccagcg tcgcgcgctt ccgccggacg
     5221 gcattcccat cggtaacctg caggcaggct atagctggat ttcacaatat caaaatgaat
     5281 tgaccgcgct cagctttcca tccgatggcg acgaaactgc gccgtatgcg ttttatgatc
     5341 gctgggcgga cacttttaat ctcaccaccg aatttgtgat cttgaaccag ggccgcagtc
     5401 ttgcgtatct cgcctggctt atggcccgca cgccgcttaa aaatcagcca tggagatccg
     5461 ccgacgctcg catc
//
